LOCUS       DADTFG010000001       131440 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.001, whole genome shotgun sequence.
ACCESSION   DADTFG010000001 DADTFG010000000
VERSION     DADTFG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 131440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 131440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..131440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.001"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(98..298)
                     /locus_tag="J1138_000001"
                     /pseudo
     CDS             complement(98..298)
                     /locus_tag="J1138_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            402..2552
                     /gene="dinG"
                     /locus_tag="J1138_000002"
     CDS             402..2552
                     /gene="dinG"
                     /locus_tag="J1138_000002"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBA8451856.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2580..3542
                     /gene="ybiB"
                     /locus_tag="J1138_000003"
     CDS             2580..3542
                     /gene="ybiB"
                     /locus_tag="J1138_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBA8451857.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNSEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            3683..4768
                     /locus_tag="J1138_000004"
     CDS             3683..4768
                     /locus_tag="J1138_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HBA8451858.1"
                     /translation="MVSGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKARQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDEDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(4997..5257)
                     /locus_tag="J1138_000005"
     CDS             complement(4997..5257)
                     /locus_tag="J1138_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA8451859.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5522..5788)
                     /gene="ybiI"
                     /locus_tag="J1138_000006"
     CDS             complement(5522..5788)
                     /gene="ybiI"
                     /locus_tag="J1138_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBA8451860.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     LIPQARREAIPGVRLCIHCQQEKDLQKLAYTGYNRRGSKDSQLR"
     gene            complement(5862..6539)
                     /gene="ybiX"
                     /locus_tag="J1138_000007"
     CDS             complement(5862..6539)
                     /gene="ybiX"
                     /locus_tag="J1138_000007"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBA8451861.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6755..9037)
                     /locus_tag="J1138_000008"
     CDS             complement(6755..9037)
                     /locus_tag="J1138_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HBA8451862.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRMANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DLSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9302..9562)
                     /locus_tag="J1138_000009"
     CDS             complement(9302..9562)
                     /locus_tag="J1138_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA8451863.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            9838..10764
                     /gene="rlmF"
                     /locus_tag="J1138_000010"
     CDS             9838..10764
                     /gene="rlmF"
                     /locus_tag="J1138_000010"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBA8451864.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEATGSIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10761..12986)
                     /gene="ybiO"
                     /locus_tag="J1138_000011"
     CDS             complement(10761..12986)
                     /gene="ybiO"
                     /locus_tag="J1138_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBA8451865.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13103..13825)
                     /gene="glnQ"
                     /locus_tag="J1138_000012"
     CDS             complement(13103..13825)
                     /gene="glnQ"
                     /locus_tag="J1138_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBA8451866.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13822..14481)
                     /gene="glnP"
                     /locus_tag="J1138_000013"
     CDS             complement(13822..14481)
                     /gene="glnP"
                     /locus_tag="J1138_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBA8451867.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14620..15366)
                     /gene="glnH"
                     /locus_tag="J1138_000014"
     CDS             complement(14620..15366)
                     /gene="glnH"
                     /locus_tag="J1138_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBA8451868.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKINGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15770..16273)
                     /gene="dps"
                     /locus_tag="J1138_000015"
                     /gene_synonym="pexB"
     CDS             complement(15770..16273)
                     /gene="dps"
                     /locus_tag="J1138_000015"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBA8451869.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(16573..17460)
                     /gene="rhtA"
                     /locus_tag="J1138_000016"
     CDS             complement(16573..17460)
                     /gene="rhtA"
                     /locus_tag="J1138_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBA8451870.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGTLQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            17695..17760
                     /locus_tag="J1138_000017"
     CDS             17695..17760
                     /locus_tag="J1138_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8451871.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            17813..18328
                     /gene="ompX"
                     /locus_tag="J1138_000018"
     CDS             17813..18328
                     /gene="ompX"
                     /locus_tag="J1138_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBA8451872.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18377..19960)
                     /locus_tag="J1138_000019"
     CDS             complement(18377..19960)
                     /locus_tag="J1138_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBA8451873.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDNLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDNFDPKP"
     gene            complement(20232..20360)
                     /gene="mntS"
                     /locus_tag="J1138_000020"
     CDS             complement(20232..20360)
                     /gene="mntS"
                     /locus_tag="J1138_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBA8451874.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20546..21013
                     /locus_tag="J1138_000021"
     CDS             20546..21013
                     /locus_tag="J1138_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HBA8451875.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21010..22128
                     /locus_tag="J1138_000022"
     CDS             21010..22128
                     /locus_tag="J1138_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBA8451876.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGGQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(22186..23106)
                     /gene="ldtB"
                     /locus_tag="J1138_000023"
     CDS             complement(22186..23106)
                     /gene="ldtB"
                     /locus_tag="J1138_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA8451877.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23325..24917
                     /locus_tag="J1138_000024"
     CDS             23325..24917
                     /locus_tag="J1138_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBA8451878.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25117..25932)
                     /gene="ybiV"
                     /locus_tag="J1138_000025"
     CDS             complement(25117..25932)
                     /gene="ybiV"
                     /locus_tag="J1138_000025"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBA8451879.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(26078..28510)
                     /locus_tag="J1138_000026"
     CDS             complement(26078..28510)
                     /locus_tag="J1138_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBA8451880.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGSIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(28516..29415)
                     /locus_tag="J1138_000027"
     CDS             complement(28516..29415)
                     /locus_tag="J1138_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBA8451881.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPDVIERALNGLLIHREKLTPVHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYISPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGCNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            29546..30208
                     /gene="fsa"
                     /locus_tag="J1138_000028"
     CDS             29546..30208
                     /gene="fsa"
                     /locus_tag="J1138_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBA8451882.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(30396..31145)
                     /gene="moeB"
                     /locus_tag="J1138_000029"
     CDS             complement(30396..31145)
                     /gene="moeB"
                     /locus_tag="J1138_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBA8451883.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     VTVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(31145..32380)
                     /gene="moeA"
                     /locus_tag="J1138_000030"
     CDS             complement(31145..32380)
                     /gene="moeA"
                     /locus_tag="J1138_000030"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBA8451884.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVSSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADMVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASCLKKTPGRLDFQRGVLQRNADGELEVTTTGQQGSHIFSSFSLGNCFIVLERERGN
                     VEAGEWVEVEPFNALFGGL"
     gene            32584..33549
                     /gene="iaaA"
                     /locus_tag="J1138_000031"
     CDS             32584..33549
                     /gene="iaaA"
                     /locus_tag="J1138_000031"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBA8451885.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            33536..35407
                     /gene="gsiA"
                     /locus_tag="J1138_000032"
     CDS             33536..35407
                     /gene="gsiA"
                     /locus_tag="J1138_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBA8451886.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQVVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAVVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            35427..36965
                     /gene="gsiB"
                     /locus_tag="J1138_000033"
     CDS             35427..36965
                     /gene="gsiB"
                     /locus_tag="J1138_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBA8451887.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKNVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            36983..37903
                     /gene="gsiC"
                     /locus_tag="J1138_000034"
     CDS             36983..37903
                     /gene="gsiC"
                     /locus_tag="J1138_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBA8451888.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            37906..38817
                     /gene="gsiD"
                     /locus_tag="J1138_000035"
     CDS             37906..38817
                     /gene="gsiD"
                     /locus_tag="J1138_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBA8451889.1"
                     /translation="MRLFNWRRQAALNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            38994..41342
                     /locus_tag="J1138_000036"
     CDS             38994..41342
                     /locus_tag="J1138_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA8451890.1"
                     /translation="MLSLYEKIKIRLIILFLLAAMSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDLLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTDLVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERANKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIATVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRNH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRTILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRV
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAVKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            41350..42678
                     /locus_tag="J1138_000037"
     CDS             41350..42678
                     /locus_tag="J1138_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA8451891.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(42725..44050)
                     /gene="rimO"
                     /locus_tag="J1138_000038"
     CDS             complement(42725..44050)
                     /gene="rimO"
                     /locus_tag="J1138_000038"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBA8451892.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            44263..44646
                     /gene="bssR"
                     /locus_tag="J1138_000039"
     CDS             44263..44646
                     /gene="bssR"
                     /locus_tag="J1138_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBA8451893.1"
                     /translation="MFVDRQRIDMLNRLIDARIDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            44757..45872
                     /locus_tag="J1138_000040"
     CDS             44757..45872
                     /locus_tag="J1138_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HBA8451894.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(45869..46495)
                     /locus_tag="J1138_000041"
     CDS             complement(45869..46495)
                     /locus_tag="J1138_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBA8451895.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            46741..47943
                     /gene="dacC"
                     /locus_tag="J1138_000042"
     CDS             46741..47943
                     /gene="dacC"
                     /locus_tag="J1138_000042"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBA8451896.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(47990..48748)
                     /gene="deoR"
                     /locus_tag="J1138_000043"
     CDS             complement(47990..48748)
                     /gene="deoR"
                     /locus_tag="J1138_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBA8451897.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(48806..49402)
                     /gene="ybjG"
                     /locus_tag="J1138_000044"
     CDS             complement(48806..49402)
                     /gene="ybjG"
                     /locus_tag="J1138_000044"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBA8451898.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            49687..50919
                     /gene="mdfA"
                     /locus_tag="J1138_000045"
     CDS             49687..50919
                     /gene="mdfA"
                     /locus_tag="J1138_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBA8451899.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(50960..51244)
                     /locus_tag="J1138_000046"
     CDS             complement(50960..51244)
                     /locus_tag="J1138_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8451900.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(51330..52145)
                     /gene="ybjI"
                     /locus_tag="J1138_000047"
     CDS             complement(51330..52145)
                     /gene="ybjI"
                     /locus_tag="J1138_000047"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBA8451901.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            complement(52145..53353)
                     /locus_tag="J1138_000048"
     CDS             complement(52145..53353)
                     /locus_tag="J1138_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8451902.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            53437..53973
                     /locus_tag="J1138_000049"
     CDS             53437..53973
                     /locus_tag="J1138_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA8451903.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIAALAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            complement(54078..55130)
                     /locus_tag="J1138_000050"
     CDS             complement(54078..55130)
                     /locus_tag="J1138_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8451904.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPNGDKVAAAVGITP"
     gene            complement(55317..55508)
                     /locus_tag="J1138_000051"
     CDS             complement(55317..55508)
                     /locus_tag="J1138_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8451905.1"
                     /translation="MLGKVFFVVLSCSLLLNPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
     gene            complement(55524..56093)
                     /locus_tag="J1138_000052"
     CDS             complement(55524..56093)
                     /locus_tag="J1138_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBA8451906.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEITIRDITRLPGGRIFV
                     EGGNRAFECKIEDVEMIGKIISLTIKYVR"
     gene            56219..56440
                     /locus_tag="J1138_000053"
     CDS             56219..56440
                     /locus_tag="J1138_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBA8451907.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            56473..56982
                     /locus_tag="J1138_000054"
     CDS             56473..56982
                     /locus_tag="J1138_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HBA8451908.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            56990..57190
                     /locus_tag="J1138_000055"
     CDS             56990..57190
                     /locus_tag="J1138_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HBA8451909.1"
                     /translation="MLTNEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELL
                     AELSTKKRGNKWLLKALRRLFH"
     gene            57154..57495
                     /locus_tag="J1138_000056"
     CDS             57154..57495
                     /locus_tag="J1138_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HBA8451910.1"
                     /translation="MAIEGAAATVPLRPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            57563..57796
                     /locus_tag="J1138_000057"
     CDS             57563..57796
                     /locus_tag="J1138_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HBA8451911.1"
                     /translation="MRNIETLTTKTGPDDAGLNLLLTEARLEERRARAEAMAARLDSL
                     ACHITSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            57796..58023
                     /locus_tag="J1138_000058"
     CDS             57796..58023
                     /locus_tag="J1138_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBA8451912.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCVECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            58020..58874
                     /locus_tag="J1138_000059"
     CDS             58020..58874
                     /locus_tag="J1138_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HBA8451913.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCEAFISRARALFEEANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLKVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASILEHRSSEGHPVIV
                     SNSNTSLTRSLYRNFTHHYIRAKRSIGVAAGEGKFATEMIATKSANWFSADFSRGRDS
                     TVIFGVQV"
     gene            58880..59701
                     /locus_tag="J1138_000060"
     CDS             58880..59701
                     /locus_tag="J1138_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8451914.1"
                     /translation="MHHGIHHFHGTPVWGSAGDVHRIAVSGAGAFVSYVRPDQIAASI
                     QHAQVVGIDNGAFSAWVRGLKINWSDFYKWLLNYYHHPKVAFFVIPDVVDGGERDNDA
                     LINEVPKMFYGKATPVWHLHESIDRLIELCREWPRVCFGSSGEYAAIRTAHWHRRMQD
                     AFEAIYCRHNFKTAVHGLRMLDGRVLGNYPLATADSTNLACNVPKFNSKYPELTRAIQ
                     EAEYSRNLTEKELKAVILKNRCAILKGAIEAVRPPSVSDWLSNGLQPSQLELEIA"
     gene            59701..62073
                     /locus_tag="J1138_000061"
     CDS             59701..62073
                     /locus_tag="J1138_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBA8451915.1"
                     /translation="MNYSYSWNAEKKAINPYVETEEQSSVSALSNLIALYAADNEQEQ
                     LRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQRFNPDMVILADVNAQ
                     PSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDCQLSTSFRFMASHE
                     GLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYVTDDVKPEEIRKTWEKVAA
                     ETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQ
                     LRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDGDTLDMEDVVNASSS
                     NPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWTNATVRQ
                     SSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLR
                     KFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGK
                     SLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAARQQGDKKAGAPVLDN
                     PRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIW
                     SPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCPLAENLNQQGKDKSA
                     DGDSRTDITRMNDKELHDYLHSMSKKERRELAARLRQVKPKRRKDYKQRITDHQRQQL
                     VYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDDKWRNLY"
     gene            62226..62414
                     /locus_tag="J1138_000062"
     CDS             62226..62414
                     /locus_tag="J1138_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8451916.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            62425..62658
                     /locus_tag="J1138_000063"
     CDS             62425..62658
                     /locus_tag="J1138_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HBA8451917.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            62961..64394
                     /locus_tag="J1138_000064"
     CDS             62961..64394
                     /locus_tag="J1138_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBA8451918.1"
                     /translation="MIQPKVFISYSWSSKTHQQHIKDIAERLAADGVETVIDIYDLKE
                     GDDKNYYMERMVQDETVTHVLVICDKKYSEKADLRKDGVGVESMIISQEIYSSVSQSK
                     FIPLIFEYKDNGEPYTPIFLKSRIYIDFSTPEKENDNWERLIRLLYGKPEFTKPPLGK
                     PPVYLEQDTSKPTYEIHAKFQTLKSAVLNQKQTLKDCRRQFLEVCRNYCISLQVVTNP
                     TTEDFAAEVLQIHKELIAVRDAITDWVLLEGDTQGEDFSKALLQFMEVMLAIRNRPKN
                     VNSYNEIWFLPHKIFAYETFLYILAALIKIEAFQHVHTLLHTSYLLPDHITSPGMEFA
                     NYSELYLSSDYLQSKLSPENYRLYSPVAELVKQSATRDDVSFDDLKQADLVALMISFI
                     NPSIFWYPQMLLYSGHYEKYPLFTRAIQHRGFKSIAVITGIDDSKLLAQKLTEGEAQR
                     NTSNWYHFGFNRDFLNQMNVSRLDSIE"
     gene            complement(64429..65469)
                     /locus_tag="J1138_000065"
     CDS             complement(64429..65469)
                     /locus_tag="J1138_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA8451919.1"
                     /translation="MSKRKNTKQVTVDHDTKPSNGPTAEAFSFGDPIPVLDRRELLDY
                     VECVQMDRWYEPPVSFNGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFARF
                     VQDYLVFGNAYLEKRTNRVGGVLSLEPALAKYTRRGVDLDTYWFVQYGMTTQPYEFTQ
                     GNIFHLLEPDINQEIYGLPGYLSAIPSTLLNESATLFRRKYYINGSHAGFIMYMTDAA
                     QNQEDVNNIRQAMKSAKGPGNFRNLFMYSPNGKKDGIQIIPLSEVAAKDEFLNIKNVS
                     RDDMMAAHRVPPQMMGIIPNNTGGFGDVEKASRVFVRNELMPLQKRLQELNDWLGEEV
                     IRFEPYTLDIEN"
     gene            complement(65466..66173)
                     /locus_tag="J1138_000066"
     CDS             complement(65466..66173)
                     /locus_tag="J1138_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase-like family protein"
                     /protein_id="HBA8451920.1"
                     /translation="MPPIPTNLMTIAGLPVDSFFFAYQYEWNTGPKSRNRVLTKMRQA
                     GADFFFSYEALNDALHTGRNQIFLCCSTASAQAIKACVSAFLSQAAAYTHTGKIKSSK
                     SHLELPNGAVIYFTELSSLTSALSGNVYVSEYAWSESPRNMIAIAKGISMHAHYHATY
                     YTTPSPNPEARQEYKKLCRNNSVTSMVFTADDAAASGAMLITDNWLNDMKKELSEEDW
                     KMLFMCEWPLASKELAE"
     gene            complement(66173..67939)
                     /locus_tag="J1138_000067"
     CDS             complement(66173..67939)
                     /locus_tag="J1138_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HBA8451921.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPHRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            68082..68915
                     /locus_tag="J1138_000068"
     CDS             68082..68915
                     /locus_tag="J1138_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBA8451922.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNATADTLTN
                     C"
     gene            68932..69990
                     /locus_tag="J1138_000069"
     CDS             68932..69990
                     /locus_tag="J1138_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HBA8451923.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSASFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAEAGA"
     gene            69994..70644
                     /locus_tag="J1138_000070"
     CDS             69994..70644
                     /locus_tag="J1138_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HBA8451924.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGMDM
                     PDQSRARLHKAIGAVLSENNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            70740..71204
                     /locus_tag="J1138_000071"
     CDS             70740..71204
                     /locus_tag="J1138_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBA8451925.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAESSGDLWRDARWAISRVQDAPHCTVELI"
     gene            71204..71407
                     /locus_tag="J1138_000072"
     CDS             71204..71407
                     /locus_tag="J1138_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8451926.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            71411..71626
                     /locus_tag="J1138_000073"
     CDS             71411..71626
                     /locus_tag="J1138_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8451927.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            71607..72119
                     /locus_tag="J1138_000074"
     CDS             71607..72119
                     /locus_tag="J1138_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA8451928.1"
                     /translation="MNPSIVKRCLIGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERAMERCVKQQPPQK
                     VYDATVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            72121..72498
                     /locus_tag="J1138_000075"
     CDS             72121..72498
                     /locus_tag="J1138_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8451929.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALAALIKNGPLWQELRASLLKTITRKENVFTSWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            72495..72923
                     /locus_tag="J1138_000076"
     CDS             72495..72923
                     /locus_tag="J1138_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HBA8451930.1"
                     /translation="MNRLLLVVLTLLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSTLLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRKRPALTGGMAYRQWLSASDAVSAGSGNAAH"
     gene            73019..73450
                     /locus_tag="J1138_000077"
     CDS             73019..73450
                     /locus_tag="J1138_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8451931.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVTTGASSMSW
                     EYRYTLNAVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAIAEPDAPEEMWTVKRG"
     gene            73443..73889
                     /locus_tag="J1138_000078"
     CDS             73443..73889
                     /locus_tag="J1138_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBA8451932.1"
                     /translation="MAELQKVDDWLSALLANLEPASRSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSGYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTAASTDSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            73958..74536
                     /locus_tag="J1138_000079"
     CDS             73958..74536
                     /locus_tag="J1138_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBA8451933.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGDLETNWIN
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAIYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITMKTSEFVLEADHTRINSEVVINGGVT
                     QGGGVMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            74533..74892
                     /locus_tag="J1138_000080"
     CDS             74533..74892
                     /locus_tag="J1138_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBA8451934.1"
                     /translation="MTLYSGMNNTSGKAITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVGLTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            74879..75787
                     /locus_tag="J1138_000081"
     CDS             74879..75787
                     /locus_tag="J1138_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBA8451935.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVTRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAGAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     QADVVLNKTQAASCTQWSVTNGGTDE"
     gene            75780..76385
                     /locus_tag="J1138_000082"
     CDS             75780..76385
                     /locus_tag="J1138_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBA8451936.1"
                     /translation="MNSLLPPGSTSLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            76382..77938
                     /locus_tag="J1138_000083"
     CDS             76382..77938
                     /locus_tag="J1138_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8451937.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGALPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKALELK
                     VYADDQMAKHLAAPDPHSQYAPKASPTFTGTPKAPTPAAGNNTTQVATTAFVQAALTA
                     LINGAPATLDTLKEIAAAINNDPNFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAGMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLLTYLRLGAGAPAIGIPFFWPSSAMPNTVMPEWADMVFLKFNGATFS
                     ATTYPKLALVFPGLTLTEARGEFLRVWDDGRGIDSGRQLLSAQGDAIRNLTGQFFAGT
                     SGNSQYVLSGANGVFSQGGMTFGPNLAGASGTGDQRSAGVDFNASRQVPTASENRPRN
                     IAFNFLVRAK"
     gene            77938..78531
                     /locus_tag="J1138_000084"
     CDS             77938..78531
                     /locus_tag="J1138_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA8451938.1"
                     /translation="MKPVFDENGLATEPGEIRCYYYDAVSYEYQGWSDEYINTGVSMP
                     ACSTGIDPSENIPGKVAVFTGKGWSHEEDHRNETVYSTENGAAVTVDYIGAIKDGYVT
                     LSPLTPYDKWDGEKWVTDIEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQPENTRLNAVLDYIDAVTATDTSTVPDVIWPELPEA"
     gene            complement(78503..78922)
                     /locus_tag="J1138_000085"
     CDS             complement(78503..78922)
                     /locus_tag="J1138_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA8451939.1"
                     /translation="MDRYFYSQKENVFFTDLNKAPSDVVEITTDEWLLLLDGQDNGMK
                     IVSNQEGYPVLTEQPPLSKENLIALADLKKRKLINEAHEHMNSRQWPGKAAIGRLKGE
                     ELAQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(78926..79327)
                     /locus_tag="J1138_000086"
     CDS             complement(78926..79327)
                     /locus_tag="J1138_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8451940.1"
                     /translation="MMPVSCYLCIFLNVGLGEAAKRDVGTGENQIPDMSAWKRNPSSN
                     RWRKLPDGTIIQMGISASGPLGSPVNITLPISFSNTNYCVVASYDNARSGVSTMVSFA
                     ALPVSPSQFSLMSSVTEQGINPFAYWIAFGD"
     gene            79355..79921
                     /locus_tag="J1138_000087"
     CDS             79355..79921
                     /locus_tag="J1138_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA8451941.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLIDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANKINESP"
     gene            80064..81236
                     /locus_tag="J1138_000088"
     CDS             80064..81236
                     /locus_tag="J1138_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HBA8451942.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTIVVRVPQGETEDETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DQLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            81246..81761
                     /locus_tag="J1138_000089"
     CDS             81246..81761
                     /locus_tag="J1138_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBA8451943.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            81816..82118
                     /locus_tag="J1138_000090"
     CDS             81816..82118
                     /locus_tag="J1138_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBA8451944.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            82133..82252
                     /locus_tag="J1138_000091"
     CDS             82133..82252
                     /locus_tag="J1138_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HBA8451945.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            82245..85322
                     /locus_tag="J1138_000092"
     CDS             82245..85322
                     /locus_tag="J1138_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA8451946.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKASAVVAAVT
                     VGLGTLAVAMAAVLGPLAVIRLGFSVLGIKTLPSVTAAVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAAAGTVLRVLASGPLAMLRVALYAISGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPISAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAIVSRAATVPLV
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            85319..85804
                     /locus_tag="J1138_000093"
     CDS             85319..85804
                     /locus_tag="J1138_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8451947.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PENDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     L"
     gene            85801..86901
                     /locus_tag="J1138_000094"
     CDS             85801..86901
                     /locus_tag="J1138_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBA8451948.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMSLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVISITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVKIDDFEME"
     gene            86992..87210
                     /locus_tag="J1138_000095"
     CDS             86992..87210
                     /locus_tag="J1138_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="in E. coli K-12 this is in prophage P2 remnant;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8451949.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(87446..89131)
                     /locus_tag="J1138_000096"
     CDS             complement(87446..89131)
                     /locus_tag="J1138_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBA8451950.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            89401..89778
                     /locus_tag="J1138_000097"
     CDS             89401..89778
                     /locus_tag="J1138_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8451951.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(89808..90065)
                     /gene="grxA"
                     /locus_tag="J1138_000098"
     CDS             complement(89808..90065)
                     /gene="grxA"
                     /locus_tag="J1138_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBA8451952.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            90225..90512
                     /locus_tag="J1138_000099"
     CDS             90225..90512
                     /locus_tag="J1138_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBA8451953.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            90496..91218
                     /gene="nfsA"
                     /locus_tag="J1138_000100"
     CDS             90496..91218
                     /gene="nfsA"
                     /locus_tag="J1138_000100"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBA8451954.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            91279..92181
                     /gene="rimK"
                     /locus_tag="J1138_000101"
     CDS             91279..92181
                     /gene="rimK"
                     /locus_tag="J1138_000101"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBA8451955.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAVLRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            92269..92745
                     /locus_tag="J1138_000102"
     CDS             92269..92745
                     /locus_tag="J1138_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBA8451956.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            93095..94207
                     /gene="potF"
                     /locus_tag="J1138_000103"
     CDS             93095..94207
                     /gene="potF"
                     /locus_tag="J1138_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBA8451957.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            94301..95434
                     /gene="potG"
                     /locus_tag="J1138_000104"
     CDS             94301..95434
                     /gene="potG"
                     /locus_tag="J1138_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBA8451958.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            95444..96388
                     /gene="potH"
                     /locus_tag="J1138_000105"
     CDS             95444..96388
                     /gene="potH"
                     /locus_tag="J1138_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBA8451959.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGVIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIMPIMWFHKHQQKSVGEHG"
     gene            96385..97230
                     /gene="potI"
                     /locus_tag="J1138_000106"
     CDS             96385..97230
                     /gene="potI"
                     /locus_tag="J1138_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBA8451960.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            97290..97778
                     /locus_tag="J1138_000107"
     CDS             97290..97778
                     /locus_tag="J1138_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBA8451961.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            97819..98946
                     /gene="rlmC"
                     /locus_tag="J1138_000108"
     CDS             97819..98946
                     /gene="rlmC"
                     /locus_tag="J1138_000108"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBA8451962.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKAADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(98975..99706)
                     /gene="artJ"
                     /locus_tag="J1138_000109"
     CDS             complement(98975..99706)
                     /gene="artJ"
                     /locus_tag="J1138_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8451963.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(99932..100600)
                     /gene="artM"
                     /locus_tag="J1138_000110"
     CDS             complement(99932..100600)
                     /gene="artM"
                     /locus_tag="J1138_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBA8451964.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(100600..101316)
                     /gene="artQ"
                     /locus_tag="J1138_000111"
     CDS             complement(100600..101316)
                     /gene="artQ"
                     /locus_tag="J1138_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBA8451965.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAVIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(101323..102054)
                     /gene="artJ"
                     /locus_tag="J1138_000112"
     CDS             complement(101323..102054)
                     /gene="artJ"
                     /locus_tag="J1138_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8451966.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(102072..102800)
                     /gene="artP"
                     /locus_tag="J1138_000113"
     CDS             complement(102072..102800)
                     /gene="artP"
                     /locus_tag="J1138_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBA8451967.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(103018..103533)
                     /locus_tag="J1138_000114"
     CDS             complement(103018..103533)
                     /locus_tag="J1138_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA8451968.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRIGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            103659..103982
                     /locus_tag="J1138_000115"
     CDS             103659..103982
                     /locus_tag="J1138_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBA8451969.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            103979..104809
                     /locus_tag="J1138_000116"
     CDS             103979..104809
                     /locus_tag="J1138_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA8451970.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(104806..105819)
                     /locus_tag="J1138_000117"
     CDS             complement(104806..105819)
                     /locus_tag="J1138_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA8451971.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(105918..107348)
                     /locus_tag="J1138_000118"
     CDS             complement(105918..107348)
                     /locus_tag="J1138_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA8451972.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPQKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(107359..108360)
                     /gene="ltaE"
                     /locus_tag="J1138_000119"
     CDS             complement(107359..108360)
                     /gene="ltaE"
                     /locus_tag="J1138_000119"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBA8451973.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSRAQLAEVAAHWRAFLAR"
     gene            complement(108397..110115)
                     /gene="poxB"
                     /locus_tag="J1138_000120"
     CDS             complement(108397..110115)
                     /gene="poxB"
                     /locus_tag="J1138_000120"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBA8451974.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVMQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(110248..111216)
                     /gene="hcr"
                     /locus_tag="J1138_000121"
     CDS             complement(110248..111216)
                     /gene="hcr"
                     /locus_tag="J1138_000121"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBA8451975.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(111228..112880)
                     /gene="hcp"
                     /locus_tag="J1138_000122"
                     /gene_synonym="priS"
     CDS             complement(111228..112880)
                     /gene="hcp"
                     /locus_tag="J1138_000122"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBA8451976.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLMSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(113024..113923)
                     /gene="lysO"
                     /locus_tag="J1138_000123"
     CDS             complement(113024..113923)
                     /gene="lysO"
                     /locus_tag="J1138_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBA8451977.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(114417..115112)
                     /gene="aqpZ"
                     /locus_tag="J1138_000124"
     CDS             complement(114417..115112)
                     /gene="aqpZ"
                     /locus_tag="J1138_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBA8451978.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            115538..117196
                     /locus_tag="J1138_000125"
     CDS             115538..117196
                     /locus_tag="J1138_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBA8451979.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(117193..118149)
                     /locus_tag="J1138_000126"
     CDS             complement(117193..118149)
                     /locus_tag="J1138_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HBA8451980.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            118300..119415
                     /gene="macA"
                     /locus_tag="J1138_000127"
     CDS             118300..119415
                     /gene="macA"
                     /locus_tag="J1138_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBA8451981.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            119412..121358
                     /gene="macB"
                     /locus_tag="J1138_000128"
     CDS             119412..121358
                     /gene="macB"
                     /locus_tag="J1138_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBA8451982.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGKDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(121431..121655)
                     /gene="cspD"
                     /locus_tag="J1138_000129"
     CDS             complement(121431..121655)
                     /gene="cspD"
                     /locus_tag="J1138_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBA8451983.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            121978..122298
                     /gene="clpS"
                     /locus_tag="J1138_000130"
     CDS             121978..122298
                     /gene="clpS"
                     /locus_tag="J1138_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBA8451984.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            122329..124605
                     /gene="clpA"
                     /locus_tag="J1138_000131"
     CDS             122329..124605
                     /gene="clpA"
                     /locus_tag="J1138_000131"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBA8451985.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     repeat_region   124818..126046
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=124818..124845
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            126219..127202
                     /gene="cas1f"
                     /locus_tag="J1138_000132"
     CDS             126219..127202
                     /gene="cas1f"
                     /locus_tag="J1138_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1"
                     /protein_id="HBA8451986.1"
                     /translation="MSSNYLTPSDLKTILHSKRANIYYLEKCRVQVNGGRVEYVTSEG
                     KESYYWNIPIANTTALMLGMGTSVTQAAMREFAHAGVMVGFCGTDGTPLYSANEVDVD
                     VSWLSPQSEYRPTEYLQQWVSFWFVEDKRLAAAKRFQLIRLTHIDKHWSSSKMLREHA
                     FQPDVNALHTLLNRTCEEIDAAENHTQLMLVEAKLTKALYKMVSQTVGYGDFTRAKRG
                     GGIDMANRFLDQGNYLAYGLAAVAAWVTGIPHGLAVMHGKTRRGGLVFDLADLIKDAL
                     VMPQAFIAAMAGEDAQEFRQRCVNIFQQADALDVMITSLQETAQALAKADQ"
     gene            127199..130432
                     /gene="cas3f"
                     /locus_tag="J1138_000133"
     CDS             127199..130432
                     /gene="cas3f"
                     /locus_tag="J1138_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HBA8451987.1"
                     /translation="MNVLIISRCTKNARVESCRIIDQFAERTGDAAWQTVITLEGVNT
                     LRRLLRKTARRNTAVACHWLKNNGQTELMWIVGNIRRFNAQGRVPTNRTTQISLVNKE
                     ENRWQCAESIALLAAIAGLFHDFGKSGRSFQQSLTQKNTRSYQPYRHEWISLRLFQTF
                     VGDRPDDVWLAKLGELTADDESAILERLQKDTPQFSNSPFSSLQPLAKTVGWLILSHH
                     RMPENLSPQSEQSCKGCESWLEKQLNADWNAVNHRRDDWSQQDFAHVWQFPSGTPLQS
                     ASWREKARQIARRASNALGLKTYGQMEQLFPLHMARFCLMLADHYYSSSDAQQKWQDP
                     DYIVWANSDRQSGTLKQRVDEHLVGVAHHAYLLGRQLMHTRASLPAIARHKGFSQRAT
                     DVNYRWQDKAWDVARALRERSREEGFFGVNMASTGCGKTFANARIMYALADEREGCRF
                     TVALGLRTLTLQTGDALRSRLGLGEDELAVLIGSAAVNQLWQQEKIDNDCGSASQESP
                     AEEQQFVKYEGSLHTGALEKWLKDDSKLKELVSAPILVSTIDHLISATEGVRGGKQLP
                     AMLRLLTSDLVLDEPDDFDIADLHAVCRLMNWAGMLGTRVLLSSATLPPGLIQALFAA
                     YLAGRKMWQASCGINGRPVNICCAWFDEKDADATQIYDGPGFRDAHAKFVARRAVMLA
                     EKERLHFGRVASVSSASGAIQDVTESVAQTVHTQMLKLHQAHRQRHESGKTVSLGLVR
                     FANINPLVAVTKALIAIPSPEDVCIHYCVYHSRHPLAVRSDIEKRLDRAFTRHNELDF
                     WNNEDIADALHNRPESHHLFVVLGTSVIEVGRDWDADWGIIEPSSMRSIIQFAGRIQR
                     HRRNVPTSENLVILRSNIKSLQKKAPAFCKPGFETKEHLLNSHDICELIPIADYRTIN
                     ALPRILSANNANKLAELEHSRLSAELMSSKSKHVVAAQWWRAPLSWNGWMQRQTPFRY
                     SPLPEAVFFLHMDDEESEARFYSRTGDNERKAQGNFRREEVRCASGVACWGVMDYQHV
                     LLNLADKQSCEIANVGEKFAEVRVPVSEEDAVDDWRYHPWLGVFRNM"
     gene            130762..>131440
                     /gene="csy1"
                     /locus_tag="J1138_000134"
     CDS             130762..>131440
                     /gene="csy1"
                     /locus_tag="J1138_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HBA8451988.1"
                     /translation="MEKTALIQFITEYIASRRQPKIDAFEKEAAKRVEQGEDASVIAQ
                     ERQELEARYLPRNWLTDAAKRAGQIKLVTHGAKFSHGDSKASSFYLETSANESYLNTA
                     SLANVATDAIGNAAALDVAKLLQTDVNGDSLLASLKRGDYQALSTFAEDKVQLELWVA
                     GFSQAWTTGQPSSHKLAKQIYFPVADGYHLLCPLFSSSLAQAMYEKLTAGRFSEESKA
                     IRDARKAG"
BASE COUNT        32169 a        32261 c        35320 g        31690 t
ORIGIN      
        1 gcgccgcatc cggcatttca caacaagcac ttgtcagcca tttgcgagcg cggcaaaaag
       61 atttctctgt tttaacgccc acaagccacc cgatgcgtta cttctccgca gccaattgtc
      121 cgcgcaacgc cattaaaagg taccccaggc gattcttgcc ctgaccatta ccgctatctc
      181 cccagtaagc atcatttggc gtatgctcaa ccagttttgc ggacgcggtt gccagcaaga
      241 gtgtacgcag ttctgcatgt tgttcgaatt tagcgcgaag cgctttacgc atcatcctgc
      301 tggttattgc gagccgcttt ccagaaacag aaaaaccatt acccctgaaa accgaaaaat
      361 gccacaatat tggctgttta tacagtattt caggttttct catggcatta accgccgcgc
      421 ttaaagcgca aattgccgcc tggtataagg cgcttcagga acagatcccc gactttattc
      481 cccgtgcgcc gcagcggcag atgattgcgg acgtcgccaa aacgctggcc ggagaagaag
      541 ggcggcatct ggcgattgaa gcccctaccg gcgttgggaa aacgctctcc tatttgatcc
      601 ccggcatcgc cattgcccgc gaagagcaaa aaacgctggt ggtaagtacc gccaacgtgg
      661 cattgcagga tcagatctac agcaaagatt taccgctgct gaaaaagatc attcccgatc
      721 ttaaattcac tgccgctttt gggcgtgggc gctacgtttg cccgcgtaat ctgacagcgc
      781 ttgccagcac tgagcccacg caacaggatc tgctggcgtt tcttgacgac gaactgacgc
      841 caaataatca ggaagagcaa aaacgttgtg cgaagctgaa gggcgatctc gacacttata
      901 aatgggatgg gctgcgcgat catactgata tcgctataga tgatgatctc tggcgtcgtt
      961 taagtaccga caaagccagc tgcctcaacc gcaactgtta ctactatcgt gaatgcccgt
     1021 tttttgttgc tcgtcgggag attcaggaag cggaagtggt ggtggcaaac catgcgctgg
     1081 tgatggcggc gatggaaagc gaagccgtat tgcctgaccc gaaaaattta ctgttggtgc
     1141 tggacgaagg tcatcacctg ccggatgtgg cgcgggatgc gctggagatg agcgccgaaa
     1201 tcactgcgcc ctggtaccgg ctccaactgg acctgttcac caaactggtc gccacctgca
     1261 tggagcagtt tcgcccgaag accatcccac cgctggcgat ccctgaacgt ttgaatgcgc
     1321 attgtgaaga gctgtatgag cttatcgcct cgttaaacaa cattctcaat ctctacatgc
     1381 ctgccgggca ggaggcagag caccgttttg cgatgggcga actgcctgat gaagtgctgg
     1441 agatctgcca gcggctggca aaactcaccg agatgctgcg tggtctggcg gagttatttc
     1501 ttaacgattt aagtgagaaa accggcagcc atgacattgt acgcctgcat cggttgattt
     1561 tgcagatgaa ccgcgcgttg gggatgttcg aggcgcaaag caaactctgg cggctggcgt
     1621 ctctggcgca atcttccggt gcgccggtga ccaaatgggc gacgcgggaa gagcgcgaag
     1681 ggcagctaca cctttggttt cactgcgtgg gaatacgtgt cagcgatcag ctggaaaggc
     1741 tgctgtggcg cagtattccg cacattattg ttacctccgc aaccttgcgt tcgctgaaca
     1801 gtttttcgcg tttgcaggag atgagtgggc tgaaagagaa agcgggcgac cgttttgtgg
     1861 cactggattc cccttttaac cactgtgaac agggcaaaat tgttattccc cggatgcgcg
     1921 ttgagccttc catcgacaac gaagagcagc acattgccga aatggcggcc tttttccgtg
     1981 aacaggtgga gagcaaaaaa catctcggta tgttggtgct gtttgccagc gggcgtgcga
     2041 tgcagcgctt tctcgactat gtgacggatt tacgcctgat gttgctggtg cagggcgatc
     2101 agccacgtta tcgcttagtt gaactgcacc gcaaacgcgt tgccaacggt gagcgtagcg
     2161 tgctggtggg cttacagtcc tttgccgaag gtcttgatct gaaaggcgat ctgctcagcc
     2221 aggtgcatat ccacaagatc gcttttccgc ccatcgacag cccggtggtg atcaccgaag
     2281 gtgaatggct gaaaagcctc aaccgctatc cgtttgaggt gcaaagcctg ccgagcgctt
     2341 cgtttaacct gattcagcag gttgggcgac tgattcgaag ccacggttgc tggggggaag
     2401 tggttatcta cgacaaacgc ttgctgacaa aaaattatgg caagcgacta ctggatgcat
     2461 taccggtatt tccgatagag caaccggaag tccctgaagg tatagttaaa aagaaagaaa
     2521 aaacgaaatc cccacgccgt cggcggcgtt aatgatgtga gtcaggtaag gagtcgtaaa
     2581 tggactatcg caaaatcatt aaagagatcg ggcgcgggaa aaaccacgcg cgcgatttag
     2641 accgggatac tgcgcgcggt ctgtatgccc atatgctcaa tagtgaagtc cctgaccttg
     2701 agttgggcgg cgtattgatt gcgctacgta tcaaagggga aggggaagca gagatgcttg
     2761 gcttttacga agccatgcaa aatcacacca tcaagctgac gccgcctgca ggcaagccga
     2821 tgccgattgt catccccagt tacaacggcg cgcgtaaaca ggccaatctg acgccgttgc
     2881 tggcgattct gctgcacaaa cttggttttc ccgtggtggt tcacggggtt agcgaagatc
     2941 caacccgcgt gctgactgaa accattttcg aactgatggg tattacacca acgttgcacg
     3001 gcgggcaggc gcaggcgaag ctcgacgaac atcaacctgt ctttatgcct gtcggcgcgt
     3061 tttgcccgcc gctggaaaaa caactggcga tgcgctggcg gatgggcgtg cgtaacagtg
     3121 cacataccct ggcgaaactg gcgacgccat ttgccgaagg tgaggcactg cgtctttcca
     3181 gcgtttcgca tccggaatac attggacgcg tcgcgaagtt ctttagcgat atcggcgggc
     3241 gggcgctact tatgcatggt actgaaggtg aagtgtatgc taatccgcag cgctgcccgc
     3301 agatcaatct cattgaccgt gaagggatgc gggtactgta tgaaaaacag gacactgctg
     3361 gtagcgagtt actgccacaa gcaaaagatc cggaaaccac ggcgcagtgg attgagcgtt
     3421 gccttgctgg cagcgaaccg attcccgaat cgctgaaaat ccagatggct tgctgcctga
     3481 tggctacggg tgaagcggca actatcagcg acggcctggc gcgcgttaat caggcatttt
     3541 aatttttatc tcagccggat gagccatgtc atccggcttt taccccaccg caatatgaaa
     3601 ttcctgcatc tttattgacc ttcccgcgcc cggcgtgcag cataaaaaaa caacaaacac
     3661 ataacataaa caggagttaa ccatggtaag tggtcatcgc tttgatgctc agacgctgca
     3721 cagttttatt caggctgtat ttcgtcagat gggtagtgag gaacaagaag cgaaattagt
     3781 cgccgatcat ttaatcgcgg caaacctggc agggcatgat tcacatggta ttggcatgat
     3841 cccaagctat gtgcgctcct ggagtcaggg gcatctgcaa attaaccatc atgccaaagt
     3901 cgttaaagaa gcgggggcgg cggtcacgct cgatggcgat cgcgcatttg gtcaggtcgc
     3961 ggcacacgaa gcgatggcgc tggggattga gaaagcgcgt cagcacggca ttgccgccgt
     4021 ggcgctacat aactcgcatc atatcggccg tatcggttac tgggcagaac agtgtgcagc
     4081 ggcggggttt gtctctatcc actttgttag cgtggtcggt attccaatgg tcgcgccgtt
     4141 ccacggtcgc gacagccgct ttggcaccaa tccgttctgt gtggttttcc ctcgtaaaga
     4201 taatttcccg ctgttgctcg attacgccac cagcgccatt gcatttggca aaacccgcgt
     4261 cgcctggcat aaaggcgtcc ccgtgccgcc aggttgcctg attgacgtta acggcgtgcc
     4321 gacgacaaat ccggcggtaa tgcaggagtc gccgttgggt tcgctgttga cctttgccga
     4381 acataaaggc tacgcccttg cagcgatgtg tgaaattctt ggcggggcgc tttctggcgg
     4441 taaaacgacg catcaggaaa cgttacaaac cagtcccgat gccattctta actgcatgac
     4501 cactatcatt atcaacccgg aactgttcgg cgcgccggat tgtagtgcac agaccgaagc
     4561 ctttgccgag tgggtgaaag cctcgccgca tgatgaagat aaaccgattt tgctaccggg
     4621 cgagtgggaa gtgaacacgc gtcgcgaacg gcaggagcag gggattccac tggatgcggg
     4681 aagctggcag gccatttgtg atgcggcgcg gcagattggt atgccggaag agacgttgca
     4741 ggctttctgt cagcagttag ccagctaaaa aaaagcccgt ccagtggcgg acgggcgaac
     4801 aagggtaaca taggatcaat gagggttaga gcatatgcgt ctgtcggcaa acagacaggg
     4861 aaatacttgt gctgaacgta gcgtaaacgc ctgatccagc caacggttcg gattttgtag
     4921 gtctgataag acgcggtagc gtcgcatcag gcatctgttg ccggatgcgg cgtaaacgcc
     4981 ttatctgtcc tacgaattac ttataaatta ccgcagtacc gcttaattta ttgttgttgg
     5041 tggcggaagt aatgctgtat ccactggcac cggccgccgc ggctttctca gccagtttcg
     5101 cttccagggc atcgagggtg gatgcgccat cggcagaaac cacgccgatt ttattcatgt
     5161 tctgtgcctg ggatgccgtt accggttccg ccgcgaacac gccaaatgac agagttgaaa
     5221 gagccatagc agcaacaaca gtattgatag ttttcataat taatctctcg caggtgattt
     5281 ttgtataagt gacgttgttt cgtcgatgtg atgagtatca cgttttttta cgagagataa
     5341 aatcgaagag aattgaccac cttgttcaaa taaattgatt gatatctaac ataatgataa
     5401 tccatttatt tgttgaactt atggacttgt tttatgcagc gtggcagtca cgacagcgtt
     5461 aaaggcacgg aatgccacgc aaaatacaaa ttatactcgc tatacatccc gccagttgta
     5521 gttaacgtaa ctggctgtct ttcgaacctc tgcgattata tcctgtataa gctagttttt
     5581 gtaaatcttt ctcctgctga caatgaatgc ataagcgcac gccaggaatg gcttcccgac
     5641 gggcttgcgg gatgagggct ccgcactctt cacattcatc caggctttcg ccacgcggaa
     5701 tttcaccccg agcgcgggca atcgcatctt caattgtact gttgatctgt tcgttgacgg
     5761 cgtcatcatt agcccaaccg gatgccatat cgtcctcccc atatcaatac ttgtacagtt
     5821 aagtgtagct aatataggga cgaactcggg cagttcaagc gtcagatctc cgaccattcc
     5881 cgcagcagat tatgataaag attgagcagc gacaggatct cttcactttc gccgtagcgg
     5941 cttttcagcg actgaatatt gttgtccagt tcaaacagca tggcgcgctt tttatcatca
     6001 cggatcatcg actggatcca cataaatgat gccactcgta cgccgcgggt tacgggtgtc
     6061 acgcaatgca ggctgctgga gggatacaac accagatcgc ctgccgggag ttttacccga
     6121 tgttgcccga aggtgtcatt aacgaccagt tcgccgccgt cgtagctttc tggatcgctt
     6181 aaaaacagcg tggcagaaag gtcagtgcgc atccagccgt tttgtggatg gctgcgtact
     6241 gcgccatcca catgaaaacc ataggtttca ttgttctgat agcgattaaa cagcggcgtg
     6301 gaaagggtac gcggcaaggc cgcggcaaag aacaaagcat gttggttaac cgcgttcagc
     6361 acctcatttt gcagggcggc gtacagggcg ctgcgggtgt ctacttgttg attgttctta
     6421 acttgcgcac cttgtgcgcc ggtggtgacg cgtccatcca cccattcggc ttgttccagg
     6481 tgttcgcgaa aacgggcgac gtcctgtggc gataacacgc cgggaatgtg gtacatcata
     6541 caagttctcc aaaaagtggg gcctgcgccc cacaccgatt tttaacagca ggcaatggtg
     6601 ctcaggtgcc ggatgcggcg tgaacgcctt atccggccta ccaaatgatg tgaattcagt
     6661 gtattgcaca accttgtagg cctgataagc gcagcgcatc aggcagtcct gctctcgcca
     6721 tcaacattag tgagccttgc gccccacatc tgaatcagaa atgcatatta gctgtgagca
     6781 agaaggttct tggctcgccc gggtgataac ggtagccgct cttattgatt gaggcgacgt
     6841 aatcggtatc aaacaggttg tagacgttta gctggaagtc gagattgcga ttaacccgat
     6901 accccagttt ggcgtcggcg acccaataac cttcggtaaa tgctggcgtc cccaccgcgc
     6961 cgtctgaacc tttatgcata ctgccgatat accgtgcgcc cgcaccaaca gaaatatcat
     7021 cggttgcctg atactggctc cataaggtga aggcgtgttc cggggtatac ggcagcgatg
     7081 aggaaccatc ctgggcaaca tctttgccgt ttttgatagt tgctttttgc tgggtatagc
     7141 cgccaatcat ctgccatgcg ggggtgatat ttccggccac ggatatctcg tagccttcga
     7201 cacgtttctt accatattgc gaataagtcc cgtcatcatt ttgctcaact tcattttcaa
     7261 tatcagtacg gaacagcgcg gcggtaagta gcaggcgttt atccagaacc tgccatttgg
     7321 tgccaatctc gctggtgttg gctttttgcg gtttaaaatc ggtgcggttg gcactgttac
     7381 cgctgccaga ctgcgcaagg gcgaagttgt tgccgcccgg cggctgctgg gaaacggcat
     7441 agttaatata gacattgccg ttttccgtca ggtgatacag cgccccggct ttccagttca
     7501 ccagattgcc cgacttggcg gtgtcgacgg tggtgaccgg agaacctttt gccacgccag
     7561 cagggcaggt gatggcaccg cgcccgctgc cgccgcaggc ggtagcactg tcatattcag
     7621 tatgataatt atccagacgg atcccgccgt tcagctcaaa atcacgggtg atttgcagcg
     7681 tatcgaaggc ataaattgcg aaggtatccg tctgaccatt ggcgtttgcg ccgttgcgcg
     7741 tcaggccgcc gggatgaatg ctgctgtcag gatgataaat atttaccgcc ggtaacgtca
     7801 ccggattaac gccatagtta gtctgtgttt cgcgggtaaa ttccacgccg gtactgagat
     7861 catgaccgat agcgccggta tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt
     7921 tgttactcac atctttggta ttcgccatgc gtgaccacgt ccagctattc acatcgctgg
     7981 tgggctgggt aatattcgac gctccaccca taatcgccgt catcaggtaa tcctgcttta
     8041 cgcgcgacca acgcgtagtg ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac
     8101 gcatggtagc ggtgtcggtg gtcgaatcgt cgtaatcgga atctgtgccg taaaagttat
     8161 gagtatcaac ttttccggaa tgattcaggg ctgctgttcc cgcagatggg gcagaataac
     8221 ccggtaaacc gatggtcgga atgccgccgt ctggcgtgtt gtgctgggtg acatgcagat
     8281 aattaagata caaacgattc gctgtaccaa ggccaaaagc gacagaaggg gcgacgccgt
     8341 aacgctcatt tttgactttg tcgcgtccgg catcgtgcgt tttttcgccc attacattca
     8401 ggcgcaccgc agtggtatca ccaatgacct gattgacgtc cagcgtgccg cggcggaacc
     8461 aggcgctgcc aatactggcg gaggcgtcaa tgccggaatc attgcgcggc tgtttgctga
     8521 tcatattgat cgagcctgtc ggtgcgctgc gcccgtagtc ggtgccggac ggccctttaa
     8581 ttacttcgac ctgctcggta ttgaaggtat cgcgcgagac gctgccgata tcgcgaatgc
     8641 catcgatata aatactgtta gaggtatcgg caccgcgcat ataaatggcg tcgccagtgg
     8701 tggagttacc gttctcaccc gcaaaaaacg cacccacgcc ggggacgttt ttgagcgcgt
     8761 cggtaaggtt ggttgcaccc tgatctttaa tcacttgttc agaaatcacc gtcatcgtgc
     8821 gggtagtatc cgctaccgga cgcgagaatt tcggatcggc agattgttgt ggcgcataaa
     8881 gcgaaggcgt tgatgcttcg acaaccagcg tgtcatccgc gttagcttgc ccttcggcag
     8941 cgagtgcctg tgccacaggc gtaataccaa tacaaagacc ggcaaagaac gtgagcgaat
     9001 gaaattgtct ggcagggaaa ttgcgattgt tttccatttt gcaggtgact ttttcttata
     9061 tggtgaaatt ttcactgtca cgagaaagca tctctgcgga tgcgatgcca ctgttaatcc
     9121 cgtgatagcg tccgtgcaag tgataataat tttgataagc attatcactt ggcgcgattt
     9181 tctatcaccc attgcgcgaa aaagaaatac atttatttac attgcgttca tatttgatgt
     9241 tttttgctta caaatcggac gaaaactgca tccagaggcg aatgcagtta cgttggtggg
     9301 gttatttgta gatgattgca gtaccgaaca tctggtcatt tccgcctgcg gaattaatga
     9361 cataaccttt cgcgccttgt tcgcgcgctt tttctgccag tttatcctcc agatcgctta
     9421 ggtttgatgc gccagttgcc gaaacggtac cggcgggccg tagttgacca ctgtccaggt
     9481 tactcatcgg ttgcgcggca taagccgtga gagagattcc gctcaggacg gtggcaatca
     9541 gtagtgtgag gcactttttc ataatgacat cctcataaat acaacgggta gtgccagata
     9601 agtgtaggtc gcctgcgcga tgaaaatgac gcaaaaaatt gacagagatg tgctcatttt
     9661 ttgaacgata tgtttttaca aaacaagaca aatcaatgat atgcataatg ctgattttta
     9721 tgatgaattg ccataaacca tagccatgtg aaggtttata gaaatgataa acgcagccag
     9781 atatttaccc tgtcgcgtta tgcgggtatc ttacgccgct gcttaaagga gaatgctatg
     9841 tccgcccaga aaccggggtt gcatccgcgc aaccgtcatc acagccgcta cgatctcgcc
     9901 acgctttgtc aggtcaatcc tgaactcagg caattcctca cgcttacacc cgccggggag
     9961 caaagcgtag actttgccaa tccgctggcg gtgaaggcgc tcaataaggc gttgctggcc
    10021 catttttacg ccgtagcgaa ctgggatatc cccgacggtt ttctctgccc accagtaccg
    10081 ggtcgggcgg attatattca tcaccttgcc gatttactgg cagaagcaac aggatcaatt
    10141 ccagctaatg ccagcattct ggatatcggc gttggtgcga actgtattta tccgctgatt
    10201 ggcgtacatg aatatggctg gcgttttact ggtagtgaaa ccagcagcca ggcgttaagc
    10261 agtgcgcagg cgatcatcag tgctaatccg gggcttaacc gcgccattcg tctgcgtcgg
    10321 caaaaagaga gtggggcgat ttttaacggc atcatccata aaaacgagca atacgacgcg
    10381 accttgtgta acccgccatt ccacgattcc gccgctgcgg cacgggcagg tagtgaacgt
    10441 aaacgccgta acctggggct aaacaaagac gatgcactga attttggtgg tcagcaacag
    10501 gagttgtggt gtgaaggcgg tgaagtcgct tttatcaaaa agatgattga agagagcaaa
    10561 ggcttcgcga agcaggtgat gtggtttacc tcactggtat ctcgtggtga aaacttaccg
    10621 ccgttgtatc gtgccctgac ggacgtgggc gcggtgaagg tggttaaaaa agagatggcc
    10681 caggggcaaa agcagagtcg ctttattgcc tggaccttta tgaacgacga gcagcgccgc
    10741 cgttttgtca atcgccagcg ttaaagcgtt ggttcccccg gcggcaacgg ttcagccggg
    10801 gtcgcgcccg gagcaggcag cacctgataa gtctgcactg gcgcgcgaac gcccgccagg
    10861 tcgaaatgtt ttttcacctg gctgtcgagg gcaaagcgta ccgtccactg tttgagtggc
    10921 agcgtggtga acgaaacacg cagtgtaaac gcggtattgc ttaagccgac aatcccggca
    10981 aagttcggtt caccaataac cagcccgcga atttcttcgt tttccatcaa ttctgctacc
    11041 gcatctttca gtgcctgatt ggctttatca gcatcttcat ggcgatcaac atcgtagttt
    11101 gccactaccg aaccaatacc gcggacgaag ttagcaaagg tggttatcga agaccacgga
    11161 atgatgtgat acgccccggt atcctggcgc acgcccacgg agcgaatcga catccgttcc
    11221 acagtgccgg tcaacggccc gatagtcacc aaatctccag tgttcatgcc gttttcaaac
    11281 tgaataaata ctccggtaat aatatctttc accagcgttt gcgaaccaaa cgagatagcc
    11341 agacctaatg ccccggcacc tgccagcaat ggcgcgatat tgacgccgat ttccgacaac
    11401 acaatcatga tggtgatggt actgataatc accgccagcg cgttacggaa cagcgtcagc
    11461 agggttcgcg tacgggcgct gggtagcggg cggccatgaa tatccgaagc cagccggttt
    11521 tctatcaaac tggcgagcac cgtccagcca accgccgaga agaaaagaat gagtgcgata
    11581 cggatcagga tatctacggt tttctgcccc gctccgtttt gcagccagtt ccagaaatcg
    11641 aacaatcccc atgcgctcaa cagcagcatt accgccacgc agactgtcag aatacgcgct
    11701 gttttcagtg ccgccgaaag ccatccattc agccgttttt gcagctcggg atagttacgc
    11761 tgagtatgtg gcgagagggt gatggttttc gccaaccagc gggaaaacat accggaaaca
    11821 aatgctgcga taccaataat cgccaggctg cgcaccgttg cacccatcat aaatttcagg
    11881 ctgttgcccg gatcgaacaa cgaaaagaaa aacagcacga taaaataggc gcttgccagc
    11941 cagtgccaca ccagcgcaaa ggcgcggata aacaggctga aaaaggccag cgaatgctcc
    12001 gcaaagttga gcaaatgctg ggtgatctct tttttattac gaaaaatcag gtacaacgcc
    12061 cagacggtca tgcacagcat aatgatgacg ttcgccagcg cacctatctg tacattcacc
    12121 tgattagaga taatcggcac agccacaatc agaccatagc caatcaggct gcttaaccag
    12181 ctcaggcgac gactccagta acgggcggtc tcgtcatgaa tcgtgaacgg gcgcagttca
    12241 gccacgtttg ggcaaaaaat caggcgtagt acggctttga aaaattcaat cagggcaaag
    12301 gcgttgagaa acaaactttg ttggaaagcg atggtgcgac tgcccgcatt caggttgtcg
    12361 cttaaaacct gaccgacaaa taatgtcagt gccagtaaca gcaggtcgat aataaacgcc
    12421 ccgataatca tcgccggaag ctgcaaccag ttactgcgct cacgattttt ttgccgcgcc
    12481 cactggccca ttttgcgata cagcggaagt gcgcacaggc gaatcagcca gtaaaaacca
    12541 aacactaata ccgctaacat tgaaaaatgg gtcaacgcat tgctgaaggt ttgtggatta
    12601 aacggcttat gcggggagcc ggtgatattg cgataaagtt gtccgaagcg ggcggaaagg
    12661 gcttcaccat aatggcggct gacttcggtg actttttgca gcacggtttg ctcttcaacc
    12721 agcgtcggcg gcacgatctt cggtacgggt tccgcagggg gcgtcgcggc gacggtgcgc
    12781 aactggtcga tcaactcttt acgcgaggtg tcattatcca gcacatccgc cagtgcgcca
    12841 taggccgctt ttttttgttc gatatccggt tccggggccg gttcagtcgt tgagtccgtt
    12901 gtcgttgtgg ttgtaacgcc gggtatggat accgcgtggg caggtgcgcc caacaggcag
    12961 aagaggatga acaggatcca ccgcatgact cctccagtga gaaaacagag caaaaagata
    13021 agtatagatg ctggaggctg ggcgatttta aatgagagga atctggtgtg cctccctttc
    13081 gggggaaagg gaggcagaga agttaagaga cgtgctgcaa aaattcctgc aagcgctggc
    13141 tcggcgggtt cttgatcaac acctgcggat cgccatcttc tgcaatccgg cctttgtcga
    13201 taaagatcag gcgcgaagct actttctcgg caaaaccgat ttcgtgggtc acgatcacca
    13261 tcgtcatccc ttcttcagcc agatcctgca taaccttcag cacttcatgg cgcagttccg
    13321 ggtcaagagc ggaagtcggt tcatcaaaca gcatcatttt cggcttcacc gccagcgcgc
    13381 gggcaatcgc cacacgctgc tgttgaccac cagaaagttc ggaagggtag tgatgtgcac
    13441 gctctgccag accgactttc gccagcagct cacgcgccag tttttccgcc tcttctttat
    13501 tcgcgccacg cacgcgtagc gggccaaaca tgacgttttc cagcgccgtc agatgcggga
    13561 agaggtaaaa ctgctggaac accatacccg cttcctggcg aatcaggcgc tcatcaactt
    13621 tcggatcgtt aaccttcagg ccatcgacaa tcagatcgcc ggaggtgatt tcttccagtt
    13681 tgttgatgca gcgcagcagg gtcgatttac cggaaccgga cggcccgata atcaccacga
    13741 cttcgccctg agcaatgttc aaatcgatat tgtgcagcac ctgggttgga ccaaaatgct
    13801 tggagacgtt tttaaattca atcacaggat tttcatcctt ctttccagac gacgcagaat
    13861 aaagctcagc accagggtaa taatcagata gaacaccgcc acggcgctcc agatctcaag
    13921 ggcgcggaag ttaccggcaa taatttcttg cccctgacgg gtcagttccg ccacgccgat
    13981 aacaataaac agcgaagtgt ctttaatgct gatgatccac tggttaccca gcggcggcag
    14041 catacgacgc agtgccagcg gtaaaatgac gtagcgaatg gtttcccaac gtgaaagacc
    14101 gagcgccagt cctgcttcac gaaaaccttt gtgaatagac agcaccgcac cacgcgtgat
    14161 ttccgcaatg tacgcgccgg agttgatcat gatggtgacc accgccgcag taaacgggtc
    14221 gatgcgtaaa tcgttaaacg ccatcggcag ggcgaaatag ataaacatca cctggacgac
    14281 gataggtgtg ccgcggatca cttcaataaa gaccagcgcg acgtggttgg ctatccaacc
    14341 accgaaggtg cgtgcaaaac ctgccaacaa tccgatcacc agaccgccag ccagaccgag
    14401 gaccgaaatc cacagggtca ttttggcacc ttcaatcaga agcggaatgg caggccagat
    14461 ggcactccag tcaaactgca tatgttgttc ctgttaccgt ggtgtaaata atcaaattca
    14521 gggtaattaa tggccccgga gcgggtttgc gcccttcacc ctaatcctct ccccggcggg
    14581 gagagggaac tgccagtgcg ctttacaggc gcagagttat tatttcggtt cagtaccgaa
    14641 ccattttttg tagatttcgt tgtaagttcc gttctcgcgc agggttttca acgcgccgtt
    14701 gattttgtca cgcagctcgt cgctaccttt cgggaacgca ataccgtatt gctgtgcttc
    14761 cagagagtca cctaccgctt tgaactgacc gttaccggcg gttttgatga agtacagaat
    14821 gtttggcgta tcgtgcagaa cggcgtctgc gcggttggtg cccagttcca tataggcgtt
    14881 atcgatgttc gggaactgac gcagatcttt agttttgatg tttgctttcg cgtaatcaac
    14941 ggagccagtg ccgctcttca cagcaaccac tttcccgtcg agatctttca cgcttttcac
    15001 atcgttatta ttagctttca ccatcactaa caggccgctt ttgtagtagc cgtcagagaa
    15061 atcgatcgct tttttacgct cgtcggtgat ggtaatgccc gccagcgcca gatcgacgtt
    15121 tttggtttgc agtgctggaa tgatcccact gaaatccatc ggcttcagtt cgtaatccag
    15181 cttcagttct ttagcgatgg cagcccacag atcaacgtca aagcccacgt atttatcgcc
    15241 ctgtttaaat tcaaacggaa cgaaggcagt atccgtcgcg acaactaatt ttttatccgc
    15301 ggcatgagaa gaaaccgcaa aagccagggt cagtgcagcc agtgaaactt ttaatacaga
    15361 cttcatagca tttccttttt tctatccacg ggacgatccc ctgcgtgaca ttcacatata
    15421 tgaaaaaatc gtgccagttt tgcaactcat tgatttttcg agatgcggca aaaacgtgat
    15481 gcacgattta tagggcaata ccctgaagat gcaccattct ggggcaccaa actggtgcgc
    15541 taaaattgtg cactcaacac agtgattatt tagcgtaaaa catgatgaaa aaacaatctt
    15601 tatgtaacga ttgtgtgatg atgtggatac aaaaaattta cttaaccagc aggggatagc
    15661 agatggatgc actaaataag tgcgttgagg cgggctacaa aacaaaacgg cctcctgtca
    15721 ggaagccgct tttatcgtgt actaaagttc tgcaccatca gcgatggatt tattcgatgt
    15781 tagactcgat aaaccacagg aatttatcca ggtcgcgaga cgcggcggtc aggatatctg
    15841 cggtgtcgtc atctttcgct tcgccaatcg ctttgcgtac gtcattagcg acgattgcgt
    15901 aacggtcagc cagctctttc aggtgatcct gaacgttgtg gatgtccagc gggtaacttt
    15961 tcagcggagt tttgctgttg ataacttgag tggtccccag agctacaccg cccagctgca
    16021 ctgcacgttc tgccatggta tccagatgat cgatcagtgc agtgcggaag ccatccagca
    16081 tttcatgtac ggcaatgaag ttagcgccgc gcatgttcca gtgcgcttgt ttggtaatca
    16141 aagaaagatc aataaactgg ataacctggc gattcagcaa ctctactgtt gcttttttct
    16201 cgctgtcaga gacatcgttg cgggtataaa gcagattggt cgcttttgat ttaactaatt
    16261 tagcggtact cataatttca tatcctcttg atgttatgtc ccagtaatta acgagactaa
    16321 gtatagcacc agctatgtgt tccgctattc tggctgttcc tatcacacta atagtggtaa
    16381 caagcgtgaa aaaacaaaac caataaaaac tcaatgagtt agatatatta ataagaacaa
    16441 ttcttattta tttgctcgcc aggaattttc ggaataaata tgcttttgca taattcgata
    16501 tataaagacg gcgtagagga aaagtagcga gaaaatctgc atggttatgc ataaccatgc
    16561 agaaatgtaa atttaattta tgtctaattc ttttattttg ctctctttgc gtactgtcag
    16621 cgtagaaccc attgaagcgg cgatgatagc gccgagcgcc agcagctgta tgggtgtcag
    16681 tgtttctccg aggaaaatca tcccggaaac ggcagccagc gccggttcca tgctcattag
    16741 cgtaccaaat gtgcgtgttg gcaaacgggt gagggcaatc atttccagcg aataaggcag
    16801 agcggtcgag agaatagcga cagccagacc caatggaata accgaccagt gccagagtgc
    16861 ttcaccagcc tgaagcgttc caattggcac gaaaattaac gctgcaatca acgaaccaat
    16921 tgccaccgtc gcaggaccat gttccgctcc agcgcgttgc ccacttaaaa tgtaaatagc
    16981 ccaacaagcc ccggccccca gtgccagcgc acagccggtt aaatcgacat gggaaacgtc
    17041 ttgccccagc ggtagcagga accacagacc aagaaccgcc agcacaaccc agacgaaatc
    17101 taccgggcga cgagaagaga acagtgccac cgccagtggt ccggtgaact ccagcgccac
    17161 cgcaataccc agcggtaccg tctgaataga aagataaaaa agataattca ttccacccag
    17221 cgaaacgccg taaaacaaca gcggtaatcg ttgctctttg gcaaagcgca gtcgccatgg
    17281 cttaaagaac gcgatgagga tcagcgtgcc taatgccaga cgcagcgcag tgacccccgg
    17341 tgcgcccacc agaggaaaaa gtgacttagc taacgaggct ccaccctgaa tagacgccat
    17401 ggcaacgagt aatatgacta ttggtaacca gaccggcatt ttacgtaatg aaccaggcat
    17461 cctttctccc acaaatatct aaacctaagt aaagcgtgga gtgtactgga tatacccaat
    17521 gctggttgag catttgttga aaaaattttc ccccgttttg actaaaatgc gccaggattg
    17581 atggaatcat tagtctggtg attaggaata atctggatga atgacaggga aaatatgcgt
    17641 aatacttacg cagttctctg aaaaagttat ttaaatttag atggatagcg gtgtatggaa
    17701 acgttctgtt acatgaaatg gcccgttaga catcataaat cgcgaagagt ttcccattaa
    17761 tttttgatat atttaaaact taggacttat ttgaatcaca tttgaggtgg ttatgaaaaa
    17821 aattgcatgt ctttcagcac tggccgcagt tctggctttc accgcaggta cttccgtagc
    17881 tgcgacttct accgtaactg gcggttacgc acaaagcgac gctcagggcc aaatgaacaa
    17941 aatgggcggt ttcaacctga aataccgcta tgaagaagac aacagcccgc tgggtgtgat
    18001 cggttctttc acttacaccg agaaaagccg tactgcaagc tctggtgact acaacaaaaa
    18061 ccagtactac ggcatcactg ctggtccggc ttaccgcatt aacgactggg caagcatcta
    18121 cggtgtagtg ggtgtgggtt atggtaaatt ccagaccact gaatacccga cctacaaaca
    18181 cgacaccagc gactacggtt tctcctacgg tgctggtctg cagttcaacc cgatggaaaa
    18241 cgttgctctg gacttctctt acgagcagag ccgtattcgt agcgttgacg taggcacctg
    18301 gattgccggt gttggttacc gcttctaatc acttcggtga tataaaaatc cgcctctcgg
    18361 ggcggatttt tttgttttaa ggtttcgggt cgaaattatc ggttccgaga tggttgtaat
    18421 ccaccttctg taactggaag ttggtgatgt atatcggccc ggctttcttc tcagatataa
    18481 acgggtattt gatatttatt tccttcgctt taattcccgt ccactgcgag aaaaagccta
    18541 agaagtcatt ggccgagcgg cgggctttaa tcacccgatg cgctttatcg tcgctggaaa
    18601 tgaccataaa aggcacctgg aaattttgct gatacttatc atcatgggca aggtattgca
    18661 cgtctttgcc gcgctcttta aaggccagac cgtggtcgga aaagtaaacc agcgagaagc
    18721 tgctgccgct gttgcgtaac tgatcgtaca gcttgcgcag taaattgtct gtttgcgtca
    18781 tggtatagag atagcacgac gtttcttttg attgcacaaa ggtttcgtat tttccttgtg
    18841 tccggtcgca ggcctgcgga tgcgagccca tcagatgtag aacaatcagc tgcggttgcg
    18901 agtgctcttg cgccagcact tgagcggtca tatccagtaa cgcttcgtct ttcgtgtttt
    18961 tatctgcttc aaaattacct tctttcagga agtacacttc atctgctcgt ttggcgatgc
    19021 tggcgatggc ggtatcgtat tcgccgattt ggccctggtt ggaaaaccac caggtctgga
    19081 agcccgcgcg atttgccagg gtgacaaagt tatcctgaaa ctgtggtttg ccatcgacaa
    19141 cgcgattgag cgttaagcca agcgatttct gcgtggagcc actggcggca atgtagtcag
    19201 caaatatcaa accgttaacg ctgctggcaa acggggtatt gtcccagtga ccgccaaagg
    19261 caccgagggc atcgcgacgc gcgctttctc cgatcaccac cacataggtc tgatacttcg
    19321 gcttaacggc agtgaccgtc caggtatctt tcattccgga aagttttgcc atccgttctt
    19381 gctcttcaat cacctcgtta ttattgacga tgacatcctt agcaaaacga aataccggat
    19441 agccagtatc tttcagttta aatacgccgc cccaggccag gttttgcacg ggagcaacaa
    19501 aaaatgtcgc cacactgaat accaggcaca ggctgtcgaa ggtattccag cgcgcttttt
    19561 cactctcttt ttttcgcctg atcgctatta cgccgagcgc aaaaataaat atgccgacca
    19621 ggtagctgta ccatggaaaa atcgtcagga tttcggtcga ctcttccata ttggtggagt
    19681 gcaatgccag caatgtattg aagttaggcg cgccgtaggc ctgagcaaaa gggaaataac
    19741 aagccgccac cagcgaactg ataccgacca gaactttttg tacgcgaggc aatgttcgcc
    19801 atagcaaaag caaaatagcc gtaaacgcag cggtgtagag caaactgaag gggtaaccta
    19861 agccgaggtt aattaatagc gactgtaaaa agtagaacgc agtccacggg ctaaataccc
    19921 ggctacgggt aacaagcgat tctttgaggg ttaaattcat atgccactat cgtaacaaac
    19981 gccatgtgct taccctggcg tcaagggtca atacctgcaa gagtgcgtga agaggggaaa
    20041 ggggtccgca tccgcgagcc gcaatatacg cagggctgca agaagataga gcgagccgct
    20101 gttcaggtca actactgacg aacaaatctt ttgcgaaggg gattgcaaat ccgacaaaaa
    20161 gtggggtttt tcagaccaaa acgcgttgtg cctgcataaa agcaggagtg aagcggcgta
    20221 ccgcgacgcc gctatttatc ggaaggttta tcctgctgcg gtttgttgtt gaccatatcg
    20281 cacaacatag agagcagcat taaccgtact ttaaagggag aatgactaaa cacgcgcata
    20341 cacctcttga actcattcat aagacctcct gacttgctaa tcccatcgat ccttgaggga
    20401 tgattgcatt acatacagat atagcacagg ctatattata tggctattgc taaaacgtta
    20461 attttttgta ccttcgcaac tctggtttac aatgtgcgca cgaaatgaga acgctatgta
    20521 tgcgtcacca taatgaggaa gcacaatgag tcgtcgcgca ggtacgccaa cagcaaaaaa
    20581 agtgacgcag ttagtgaacg tggaagagca cgttgaaggg ttccgccagg tcagagaggc
    20641 gcatcggcgc gagctgattg atgattacgt tgagctgatt tctgacttga tcagggaagt
    20701 gggggaagct cgtcaggtag acatggctgc tcgtctgggg gtttcgcaac cgacggtggc
    20761 taaaatgctt aagcgactgg caaccatggg gctgattgaa atgatcccct ggcgtggcgt
    20821 gtttttaacg gcagaaggag agaagctggc gcaggaaagc cgcgagcgac atcagatagt
    20881 cgaaaatttc ttgttggtgt tgggcgtcag tccggaaatc gcccgtcgcg acgcggaagg
    20941 tatggagcac catgttagtg aagagacgct ggatgccttt cgtttgttta cccagaaaca
    21001 cggtgccaaa tgagcctgcc ttttttacgc acgctgcaag gcgatcgttt ttttcagtta
    21061 ttaattcttg ttggcatcgg attaagtttt ttcgttccct ttgcaccgaa atcctggcct
    21121 gctgctatcg actggcacac catcatcacc ttaagcggtc tgatgctgct gaccaaaggt
    21181 gtggagttaa gcggttattt tgatgtgctg gggcgcaaaa tggtgcgccg ctttgctacg
    21241 gagcgacggc tggcgatgtt tatggtgctg gcggcggcgc tgctttctac ctttctgacc
    21301 aacgatgtcg cgctgtttat agttgttccg ctgactatca cgctaaaaag actgtgtgag
    21361 atcccggtta atcggctgat tatttttgag gcgctggcag tcaacgctgg ttcgctactg
    21421 acgccaattg gcaacccgca aaatattctt atctggggac gttctggtct ttcgtttgcc
    21481 ggatttattg cccaaatggc accgctggct ggcgcaatga tgctgacgct cctgctcctg
    21541 tgctggtgtt gtttccctgg aaaggcactg caataccata cgggggggca aactccggag
    21601 tggaaaccgc ggctggtgtg gagttgtctg gggctgtata tcgtctttct gacggcgctg
    21661 gagttaaaac aagagctgtg gggactggtg attgtggcgg cgggctttgc gctgctggca
    21721 cgtcgcgtgg tgttgagtgt ggactggacg ctgctgctgg tgtttatggc gatgtttatc
    21781 gacgtccatt tactgaccca gcttccagcg ttgcaaggtg tgttgggtaa cgtgagtcat
    21841 ttatctgaac ccgggttatg gttaacggca atcggtttat cgcaggtgat cagtaacgtg
    21901 ccgagtacca ttttgctgct caattatgta ccgccgtctt tattactggc atgggcggta
    21961 aacgtggggg gctttggttt attacccgga tcgctggcaa atttgattgc gttacggatg
    22021 gcgaacgatc gccgtatctg gtggcgtttt catctttatt cgataccgat gctgttgtgg
    22081 gcggcgctgg tgggatatgt tttgttggtt atgatcccgg cgggataagg tctggcaaat
    22141 taaaaaggcg gattatgagt ccgcctttgt tttacgtttt gacgattaat tcagacgaac
    22201 cggcatcccg gaacgatttt tgatcgcttc atcaagaaca acctggtcaa catctggctg
    22261 accggtcact gtctgcacgc tcttcgtcag ggtaattggc acaatttcct gaccttcaaa
    22321 ctgggcttcg gtggtagaca gcgggttatg gacttcaatg taacggctgc cgtctggttc
    22381 ggtagtcgct ttcaccggct catcaataaa ctgcacgcga gtaccgacag gcactttctc
    22441 gaacaggaat ttaatgtctt cgttacgcag acgcacacag ccatgactta cacgcaggcc
    22501 gataccgaag ttggcgttgg tgccatggat agcgtacagg cgaccgatgt agagtgcata
    22561 cagacccatc gggttatccg gacctgctgg aacgacagcc ggaagcggtt cgcccgcagc
    22621 gcggtactct gcgtgcattt tggcggtcgg cgtccaggtc gggcctgctt ttttacgctc
    22681 aactttggtg gtccagttga taggcgtgtc tttgcctaac tgaccaatgc cgatcggcag
    22741 cacgataacg gtgttggttc ctttcggata gtaatacaga cgcatctctg cactgttaat
    22801 gacgatgcct tcatgaacgg tatccggcag gatcagctgc tgcggaatgt tcagtacagt
    22861 accgcctttc ggcaggaagg tatccacacc cgggttcgct tccatcatat tggaaagtcc
    22921 catctggtac tcggcggcaa aatactccag cggctgagtg ttaccttcag gaatggtgat
    22981 cacctgattc tgaccaacca ggcgactccc gtcggttggc agaggataag ttaccgcaga
    23041 ggcggtactg caaaagccga caacagcgaa ggccgctgcg aataatgttt tcaatttcat
    23101 attcatgtta agcgagattt tgtgcctggc aggccattgg gttgagaata ttagagtatt
    23161 ggaagcgcat tataagttca ttccagctca cagtgaaatc agatgtgtac gaaatcacat
    23221 tttttgcctt tggcttgagt gtagacctta agcgaggagc aggatcttct ttcagattta
    23281 tggcataatg cgcggtttgt catatctctt ttcaggatac gcctgtgtta gtttccagta
    23341 acgtcaccat gcagttcggc agtaagccgt tgtttgaaaa catttccgtc aaatttggcg
    23401 gcggcaaccg ttacggcctg attggcgcga acggtagtgg taaatccacc tttatgaaga
    23461 tcctcggcgg cgacttagag ccgacgttgg gtaacgtttc cctcgatccc aacgagcgca
    23521 ttggtaagct gcgtcaggat cagtttgcct ttgaagagtt cactgtgctg gatacggtga
    23581 tcatggggca taaagagttg tgggaagtga agcaggagcg tgaccgcatc tatgctttgc
    23641 cggaaatgag tgaagaagac ggctataaag tggccgatct ggaagtgaaa tacggcgaaa
    23701 tggacggtta ctctgcggaa gctcgcgctg gtgaactgtt gcttggcgtg ggaattccag
    23761 tggaacagca ctacggcccg atgagtgaag ttgctcctgg ctggaagctg cgtgtgctgc
    23821 tggcgcaggc gctgtttgct gatccggaca ttctcctgct cgacgaaccg accaacaacc
    23881 tcgacatcga caccattcgc tggctggaac aggtgctgaa cgagcgtgac agcaccatga
    23941 tcatcatctc gcacgaccgt cacttcctta acatggtttg tacccacatg gcggatctgg
    24001 attacggcga gctgcgcgtt tatccgggca actacgatga gtacatgacg gcggcgactc
    24061 aggcgcgtga acgtctgctg gctgataacg ccaagaagaa agcgcagatt gctgagttgc
    24121 aatctttcgt tagccgcttt agcgccaacg cctcgaaatc tcgccaggca acttcgcgcg
    24181 cgcgccagat tgataaaatc aaactggaag aggtgaaagc ctccagccgt cagaacccgt
    24241 tcatccgttt tgaacaggat aagaaactgt tccgtaacgc gctggaagtg gaaggtctga
    24301 ctaaagggtt tgataacggt ccactgttta aaaatctcaa cctgctgctg gaagtgggtg
    24361 aaaaactggc ggtactgggt accaacggcg tcggtaaatc aacgctgctg aaaacgctgg
    24421 tgggcgatct gcaaccggac agcggcaccg taaaatggtc tgagaacgcg cgcattggtt
    24481 actacgccca ggaccatgaa tatgagttcg aaaatgatct gaccgtgttc gaatggatga
    24541 gccagtggaa gcaggaaggc gatgacgagc aggcggtacg cagcattctc ggtcgtttgc
    24601 tgttcagcca ggacgacatc aaaaagcctg cgaaagtgct ttccggtggg gaaaaagggc
    24661 ggatgctgtt tggtaagtta atgatgcaga agccgaacat tctgatcatg gacgaaccga
    24721 ccaaccacct ggatatggaa tccattgagt cgctgaacat ggcgctggaa ctgtatcagg
    24781 gcacgctgat ctttgtttct cacgaccgtg agtttgtaag ctccctggcg acccgtattc
    24841 tggaaatcac cccggaacgc gtgatcgact ttagcggtaa ttacgaagat tacctgcgta
    24901 gtaaagggat cgagtaagtt attatcagta gccctgatat ggtcagggct actgaaactg
    24961 atgacaaacg caagattgcc tggtgcgcta cgcttatcag gcctggcagt gtatcgcaat
    25021 atattgaatt ttcatgattt tgtatgccaa ataaggcggt tacgccgtat ccggcataat
    25081 gacatgcact ttctcaataa actgaattgg cgataatcaa agattaaacg gcggtttatt
    25141 ttccagcacc gcctgaatca cattcagcgc gccttcatga ttattatcat cggtagcgta
    25201 acgggcgatt tgtttaatgt tttccgcagc attgcccatc gcaaaggaat aacgcgccat
    25261 tttcagcatc tccgcatcgt taccgctgtc gccaatcgct accacatttt gcggtgacag
    25321 atcccagcgt ttcagtaacc gcgaaatacc gtttgcttta tgtagaccgg gaataatcag
    25381 gtcgataaag ccaaaaccac tggtgacggg tttcataatg ccatcgagcg ctatgtgcag
    25441 tttgtcgatc actaacggga tttgttcatc cggcaggttg agcgaaaact tgaacagtac
    25501 gtcgtcaatc tcctgataat cttttacagg tttcaggcga tggtagtgtt ttgccatcag
    25561 tgcgacgaat gcttcggggg cgttttcgct gacatatgca ctttgcagac cgcaggcgac
    25621 aaaattgagt tgcttatctt ttagcaactc gccaatcaca atccgcgatt catgtcgggt
    25681 cagttcgccg tggaacagtt gcttgccatg ttcgtaaacc agtgcgccgt tttccgcgac
    25741 aaaagagatc tcatccttta gctcaggaaa gaacgaaata agctggtaat actgattacc
    25801 gctagcgaca acgaacttaa tgccgcgctt tttcagttcc tgatattgcg ccataaaacg
    25861 tggttggttg tacgttttgg cgtcgttaag aaaagtaccg tccatgtctg tgacgataac
    25921 ttttacgctc ataggtgtgc tcctggctcg aaaatgaaac cgtaacagtg taataacaat
    25981 gtgacgcaga gcacaaatta tatttcgaat gaaagcgagg ctgaaattga tgatgtgaat
    26041 gatttagccc ggcgacgccg ccgccgggct gagaagatta cagcatatgt tcagtacggg
    26101 cgatgatatc gtcctgagcg tctggagaga gcgcggtgaa gaaggcggaa tagcccgcga
    26161 cgcgcactac cagatcgcga tactgatcgg gatgtttttt cgcctccagc agcgtctcgc
    26221 gggaaacgat gttgtactga atatgccagc ctttatgcac ttcaaagaag gtgcgcagca
    26281 ggatcatcag tttctgcttg tcagattcgt tctccagcgt tgctggattc agtttctggt
    26341 tgagcagcac gccgccgaga atcgcggccg taggcagttt acccactgag ccaatgaccg
    26401 ccgttgggcc gagatggtca gtaccggagg ccggactagc gccttctgcc agcggggtat
    26461 gcgctttacg cccgtccggt gttgccatcg tctgcgcgcc aaacggtacg ttagcggaga
    26521 tagacgacgt acccgcgtaa tagttgccgc caaccggacc acgaccgtag cgtgggttat
    26581 ggtactgttt tagttcgtcg atataggtct gataagcgcg agccagcagc gtatcgacag
    26641 tatcatcgtc gttgccgtac ttaggcgcac cgttaatcag ccgctgacgc agctgctcgt
    26701 gagtcagacc gtcgaagtca tccgccagtg cggcagccag ctgttgctga ccaatcgagc
    26761 cctgttcaaa taccagtttc ttcactgccg ccaggctgtt gccgaggtta gcaatgccga
    26821 cctgcaggcc agaaacccag tcatatttcg cgccgccttg cttgatactt ttcgctcgct
    26881 caatacagtc atcgaccagc gccgagcaga gaatatcgtg tacgttctct tccagcatgg
    26941 tgtcgacgac atattcgatt tcgattgatt tacgcgtgta gtaacggatt tgcgtatccc
    27001 atgcgtccat tacttcatcg aagttgttga agttacccgc tgacaacgct tttgcttgtg
    27061 gcaggaacac tttgccgctg gtggcatcac gaccaccttc cagcgccgcc agcatcacgc
    27121 gggcgaagtt gataaagctc atgccggtac agcgatagcc ccatttgcca ccgacggcgg
    27181 tttcgataca accaattgct gcgtagtcgt aagcgtcctg cggttcaata ccgagtttaa
    27241 taaattccgg gatgacgatt tcgtcgttgt tgaacgccgg catcccgaag ccgcagcgga
    27301 tcacctgaac acaggcgtcg aggaaatcgt tactcatacc cgcatggtaa cgcacgctga
    27361 ggttaggctg agtcgaacgc aggcgaccgc aagattcgag gatcgcataa gagagtggat
    27421 ttaccgcgtc catcggttga ccatcaacca gattttgccc gccaatggtg acgttctgat
    27481 acagcggact tcccgcagag gcttttgagt gtgagccgga gcggattttg ttcacttcca
    27541 gcagtttcag ccagcagcta tgcagcatct cgatggcgtg ttcgcgatcc agtgtctggt
    27601 tcagttcaac gtcacggcga tagtatgggt agagatactg gtccatacga ccaaacgata
    27661 ccgagtgacc gttagattcg atctgcaaaa tcaactggat gaagtagcac agttgcagcg
    27721 cctgccagaa agtctgcggc ggctggtggg cgataagatc gcagttttct gctatcgtca
    27781 gcagttcatc gcgacggctt tcgcgggttt cggttgcggc catttcacgc gccagggcag
    27841 cgaaacgttc aatgtgttca ctgactgcca ccagcacgat atcaatcgct ttcaggaatt
    27901 gctcgccgtg cagatcttcc agaaccgtca gattgatgcg cgagcgacgt tccgccactt
    27961 tctcacgcag accatcaaga cctttttcca gcagtagcgg gaaattgacc gccaggtgcg
    28021 catcgccgga ggtcatattg ccctccgctt taatgatgcc ggtcgccagc agaccttttt
    28081 gctcatcggt aaacatgccg tagcagcgat cctgtacggt ctgaccgcgc caccacgggc
    28141 acacttcatg cagaacgcgt ttgttctctt cgctcaccgc aaaaccagca ccgggacgat
    28201 ctgccagatc atcaatctct ttttcgatcc aggagacggt atattccggg aagatcggcg
    28261 cggcgcgaac ttcgcttgcc tggttgccaa tgatcaactc atcgtgcttg atccagatgg
    28321 tgcgattcgc caggtgatgc gccagtgcca gcgcgcgacg taccgggatt ggcttatcga
    28381 gatgttgttg atacatctcg gtatagtgtt gtgctcgttc ggtacagacc ggcggtttca
    28441 caatatgcac cagcgcattt ttgtgcgctt taatgcggtc gctgagcgta tccagtttta
    28501 gtgttgtcat gattgttatc ctcgtaaggt cgcggttaac cctttctggc aggcatactg
    28561 ctgggcaaag tcgagcagtt ctggcgcatc aagcggtttt tccggggcgt catagggcag
    28621 attaagtaag tgatatttgt tgatgcccag tgtgtggtag ggcagaaaat gaatttcgct
    28681 aacgtgcagc tcgtcggcgg caaaatcggt aatggctttc acagaagttt cgtcggcatt
    28741 acagccctga atcagtggca cgcggatgat gatttttttc cccgccgcag cgagtttttt
    28801 cagattatcc agcaccctgg cggcgttacc gtcggtccac tgtttaaacg gcgcgtcggc
    28861 gacgtgtttt aaatcggcaa gaaacagatc gatatagggc agagaagggg agatatattt
    28921 ccacggcaca tgcagacagg tttctaccgc agtatgaatg ccggcctcgt ggctggcttg
    28981 cagtagcgcc gtcgccattt ccggctgcat aaacggttca ccgccagaaa gtgttaagcc
    29041 gccgccgctg cgatcgtaaa acggtttatc gcgcaaaacg gtcgccatga tctcctcaac
    29101 gcttttcact tcaccacaca cggttaacgc ctgtgtcggg cagcagtcgg ttagcgccgt
    29161 cagatgcacc ggggttaact tttcccgatg aataagcaaa ccattcaacg cgcgctcaat
    29221 cacgtccggc gcggtcttag cgcacagctc acagccttcc agacagagtc gtgggtcata
    29281 cagcagatcc tgcgtgcggg cgcggctttc cgggttctga caccagcggc agcccagcga
    29341 acagccttta agaaatacga ccgtgcggat accggggcca tcatgggtcg agtagcgttg
    29401 aatattgaaa atcatagttg cctctccgtt tcgttcaaac attaaaatac tttcgaatga
    29461 aagttagatt gatgtacgtc aactgttcag agagttttcc cgtgatagtc tacattcaga
    29521 caaaaagtac attttgagga tggttatgga actgtatctg gatacttcag acgttgttgc
    29581 ggtgaaggcg ctgtcacgta tttttccgct ggcgggtgta accactaacc caagcattat
    29641 cgctgcgggt aaaaaaccgc tggaggttgt gcttccgcaa cttcacgagg cgatgggcgg
    29701 tcaggggcgt ctgtttgccc aggtaatggc taccactgct gaagggatgg tgaatgacgc
    29761 acgtaaattg cgttctatta ttgcggatat cgtggtgaaa gttccggtga ccgctgaggg
    29821 gctggcagct attaagatgt taaaagcgga agggattccg acgctgggaa ccgcggtata
    29881 tggcgcagca caagggctac tgtcggcgct ggcaggtgcg gaatatgttg cgccttacgt
    29941 taatcgtatt gatgctcagg gcggtagcgg tattcagact gtgaccgact tacaccagtt
    30001 attgaaaatg catgcgccgc aggcgaaagt gctggccgcg agtttcaaaa ccccgcgtca
    30061 ggcgctggac tgcttactgg ctgggtgtga gtcaatcacc ctgccgctgg atgtggcaca
    30121 acagatgatt agctatccgg ctgttgacgc cgccgtagcg aagtttgagc aggactggca
    30181 aggggcgttt ggcagaacgt cgatttaacc ggacgttctg catcctcata aattgctgat
    30241 agcgtggcgg agtgcaccga tgcctgccgc gtctatcagg cctggaaatc tgcgtcagaa
    30301 ccgtaggcca gataaggcgt tcacgccgca tctggcgtct ggagcaaatg ccggatgcga
    30361 cgcttgccgc gtctcatccg gcctggaggt ggcagttact gcccgcacac ctcacacccc
    30421 ggattacgca tcagtttcat ttcgcgaaac tgacaggtca tcgcatcgta catcacgatt
    30481 tttccgctgg caggtttgcc ataaccggcc agcagtttaa tcgcttccat tgcctgcaat
    30541 gaaccaatta cgccgatcaa cggcgccatt acacccgctt ccacgcaggt taacgcgttt
    30601 tcgccaaaca aacggctaag acagcgatag caaggctcgc cgtcctgata agtaaagacg
    30661 gttacctggc cctccatacg aattgccgcg ccggaaacca gcggcacctt cgcggcaaaa
    30721 cagcctgcgt tcagttgatt acgtactgca acgttatccg tacagtcgag caccagatcg
    30781 tgttttgcaa tcatcgcagc aagttcagcg tcatcaagca gcgcattgac gggtgtaatc
    30841 gcaatatgtg ggttgatccg cgtcagggcg tcacgggcgg attctacctt cggttgcccg
    30901 accgtggcat cactgtgcag cgtctggcgt tgcagattgg agagcgaaac cgtgtcgaag
    30961 tcgagcagcg tcaggttgcc gacaccggcg cttgccagat actgcgaagc tgcgcagccg
    31021 agtccaccaa ggccgacgac cagcacacga gaatctttta gcgcctcctg accgtcaaaa
    31081 tcaaaaccgc gcagaatgat ttgccggtta tagcgcagca tctcctgatc gctgagttcc
    31141 gccattacag gcctccgaac aacgcgttaa acggttctac ttccacccat tcgcccgctt
    31201 ccacattgcc gcgttcgcgc tccagcacga taaagcagtt gccgagacta aaggagctaa
    31261 atatatgtga gccctgttgt ccggtggtcg tgacttccag ttctccgtcg gcgttgcgtt
    31321 gcagcacacc gcgctggaaa tcaagacgtc ctggcgtttt cttcagacac gatgccgtgc
    31381 gtacgcgctg acgcgcgggc aggccgctgg cagtgttgcc gcttagtttt gccagcaaag
    31441 gctgtaccag ttgatagaag gtcagcgtcg ctgaaaccgg attacccggc aggccgcaga
    31501 accagctatt agaaagttta ccgaacgcga acggtttacc cggtttaatc gccagcttcc
    31561 agaaggcgat ctctcccagc tcttcaagaa tcgttttggt gtaatccgct tcacccactg
    31621 aaacaccgcc ggaactgatc accatatccg cctggctgtc ggcttcaata aatgcggcgc
    31681 gcagggcatg gggatcgtcg cggataatcc ctaagttgat tacctcgcat cccaactgtt
    31741 ctaacatcag gtgtacggcg agacggttgg tatcgtagat ttggccgtcg cccagcggct
    31801 gaccgggcaa ctggagttca tcaccggtag aaaaaagcgc tacacgcact ttacgaatca
    31861 ccggaacttc ggcaatcccc agtgaagcaa ttactggcag ctctgcggta gtcaggcgag
    31921 tccccgccgg gaaaacaacc gcacccgcag agatatcttc accgcgacgg cgaatatttt
    31981 gcccgctact cacttcagca gtaaaacgca cgccattgtc cgtttgttca gtctgttcct
    32041 gcatgaccac cgcttcgcag ccttccggca ccggcgcacc ggtcataata cgaatgcagg
    32101 tacccgcagg ccattcacca tggtacggct gaccggcaaa tgatttaccg gcaacgggca
    32161 gcggttgccc ggaggcaata tcggctaaac gcaccgcgta gccgtccata gcggagttat
    32221 caaaccccgg gacatccagt ggcgaaacga catcgctcgc cagaatacgg ccaaaacact
    32281 gaaccagtgg cagcgtttcc tgggcggtca gtggggtaac gcgagaaagc atctcattaa
    32341 gcgcggtgtc gagcgacatc aatccggtgg taaattccat gaaaacactc ctgcggaggc
    32401 aaaatcgaat ttgcctatta tgtcagaaaa acgccacaga ctgtatgcca cctcgggcgt
    32461 agcgctggct cctgccttta catgccatat ccatctttct atattcaaaa attgaatgag
    32521 tcattcataa aaattctgat atttatagca aaagtggcga gccaccctta atggacgaat
    32581 actatgggca aagcagtcat tgcaattcat ggtggcgcag gtgcaattag ccgcgcgcag
    32641 atgagtctgc aacaggaatt acgctacatc gaggcgttgt ctgccattgt tgaaaccggg
    32701 cagaaaatgc tggtagcggg cgaaagtgcg ctggatgtgg tgacggaagc ggtgcgtctg
    32761 ctggaagagt gtccactgtt taacgccgga attggcgctg tctttacgcg tgatgaaacc
    32821 catgaactgg acgcctgtgt gatggatggt aacaccctga aagccggtgc ggtggcgggc
    32881 gttagtcatc tgcgtaatcc ggttcttgcc gcccggctgg tgatggagca aagcccgcat
    32941 gtgatgatga ttggcgaagg ggcagaaaat tttgcgtttg ctcatggcat ggagcgcgtc
    33001 tcgccggaga ttttctccac acctttgcgt tatgaacaac taatggcagc gcgcgaggaa
    33061 ggggcaacag tcctcgacca tagcggtgcg ccactggatg aaaaacagaa aatgggcacc
    33121 gtaggggccg tggcgttgga tttagacggc aatctggcgg cagccacgtc cacgggcgga
    33181 atgaccaata aattacccgg acgagttggc gatagtccct tagtgggggc cggatgctat
    33241 gccaataacg ccagtgtggc ggtttcctgt accggcacgg gcgaagtctt catccgcgcg
    33301 ctggcggcat atgatatcgc cgcgttaatg gattacggcg gattaagtct cgcggaagcc
    33361 tgcgagcggg tagtaatgga aaaactccct gcacttggcg gtagcggtgg cttaatcgct
    33421 atcgaccatg aagggaatgt cgcgctaccg tttaacaccg aaggaatgta tcgcgcctgg
    33481 ggctacgcag gcgatacgcc aaccaccggt atctaccgtg aaaaagggga caccgttgcc
    33541 acacagtgat gaacttgatg ccggtgatgt gctggcggtt gaaaatctga atattgcctt
    33601 tatgcaggag cagcagaaaa tagctgcggt ccgcaatctc tcttttagcc tgcaacgtgg
    33661 tgagacgctg gcaattgttg gcgaatccgg ctccggtaag tcagtgactg cgctggcatt
    33721 gatgcgcctg ttggaacagg cgggcggttt agtgcagtgc gataaaatgc tgttgcagcg
    33781 gcgcagtcgc gaagtaattg aacttagcga gcagagtgct gcacaaatgc gccatgtgcg
    33841 cggggcggat atggcgatga tatttcagga gccgatgaca tcgctgaacc cggtatttac
    33901 tgtgggtgaa cagattgctg aatctattcg tctgcatcag aacgccagtc gtgaagaagc
    33961 gatggtcgag gcgaagcgga tgctggatca ggtacgcatt ccggaggcac aaaccattct
    34021 ttcacgttat ccgcatcaac tctctggcgg gatgcgccag cgagtgatga ttgcgatggc
    34081 gctgtcatgc cgcccggcgg tgctgattgc cgatgagcca accaccgcgc tggatgtcac
    34141 tattcaggcg cagatcctgc aattaatcaa agtattgcaa aaagagatgt cgatgggcgt
    34201 tatctttatc actcacgata tgggtgtggt ggcagagatt gccgatcggg tactggtgat
    34261 gtatcagggc gaggcggtgg aaacgggtag cgtcgaacag atttttcatg caccgcaaca
    34321 tccttatacc cgtgcgctgt tagctgctgt tccgcaactt ggtgcgatga aagggttaga
    34381 ttatccccga cgtttcccat tgatatcgct tgaacatcca gcgaaacagg agccacccat
    34441 cgagcagaaa acggtggtgg atggcgaacc tgttttacgg gtgcgtaatc tggtcacccg
    34501 tttccctttg cgcagcggtt tgttgaatcg cgtaacgcgg gaagtgcatg ccgttgagaa
    34561 agtcagtttt gatctctggc ctggtgaaac gctatcgctg gtgggcgagt ctggcagcgg
    34621 taaatccacc accgggcggg cgttgctgcg cctggtcgaa tcgcaaggcg gcgaaattat
    34681 ctttaacggt cagcgaatcg ataccttgtc acccggtaaa cttcaggcat tgcgccgcga
    34741 tattcagttt atttttcagg acccttacgc ttcgctggac ccacgtcaga ccatcggtga
    34801 ttcgattctc gaaccgctgc gcgtacacgg tttattgcca ggtaaagaag cggctgcacg
    34861 cgttgcgtgg ttgctggagc gcgtgggtct gttaccggaa catgcctggc gttaccctca
    34921 tgagttttcc ggcggtcagc gccagcgcat ctgcattgct cgcgcgttgg cattgaatcc
    34981 aaaagtgatc attgccgacg aagccgtctc ggcgctggat gtttctattc gcgggcagat
    35041 tatcaacttg ttgctcgatc tccagcgtga tttcggcatt gcgtatctgt ttatctccca
    35101 cgatatggcc gtggttgagc ggattagtca tcgtgtggcg gtgatgtatc tcgggcaagt
    35161 tgttgaaatt ggtccacggc gcgcggtctt tgaaaatccg cagcatcctt atacgcgtaa
    35221 attactggcg gcagttccgg tcgctgaacc gtcccgacaa cgaccgcagc gtgtactgct
    35281 gtcggacgat cttcccagca atattcatct gcgtggcgaa gaagttgcag tcgtctcgtt
    35341 gcaatgcgtc gggccggggc attacgtcgc acaaccacaa tcagaatacg cattcatgcg
    35401 tagataacat tcaggcggag aataaaatgg caagagctgt acaccgtagt gggttagtgg
    35461 cgctgggcat tgcgacagcg ttgatggcat cttgtgcatt cgctgccaaa aatgtggtgg
    35521 tggcggtggg ctcgaacttc accacgctcg atccgtatga cgcaaatgac acgttatctc
    35581 aggccgtagc gaaatcgttt taccaggggc tgttcggtct ggataaagag atgaaactga
    35641 aaaacgtgct ggcggagagt tataccgttt ccgatgacgg ccttacttac accgtgaaat
    35701 tgcgggaagg cattaaattc caggatggca ccgatttcaa cgcagcggcg gtgaaagcga
    35761 atctggaccg ggccagcgat ccggcgaatc atcttaaacg ctataacctg tataagaata
    35821 ttgccaaaac ggaagcgatc gatccgacaa cggtaaagat taccctcaaa cagccattct
    35881 cagcgtttat taatattctt gcccatccgg cgaccgcgat gatttcaccg gcagcgctgg
    35941 aaaaatatgg caaggagatt ggttttcatc cggtgggaac cgggccgtat gaactggata
    36001 cctggaatca gaccgatttt gtgaaggtga aaaaattcgc gggttactgg cagccaggat
    36061 tgcccaaact ggacagcata acctggcgtc cggtggcgga taacaacacc cgcgcggcaa
    36121 tgctgcaaac cggtgaagcg cagtttgctt tccccattcc ctacgagcag gccgcactgc
    36181 tggagaaaaa caaaaatatc gagttgatgg ccagtccgtc aattatgcag cgttatatca
    36241 gtatgaacgt gacgcaaaag ccgttcgata acccgaaggt ccgtgaggcg ctgaattacg
    36301 ccattaaccg cccggcgctg gtgaaagtgg ccttcgcggg ctatgcaacg ccagctactg
    36361 gtgtggtacc gccgagtatc gcctacgcgc aaagttataa accgtggcct tacgatccag
    36421 tgaaagcgcg cgaattactg aaagaggcgg gatatcccaa cggtttcagt accacgctgt
    36481 ggtcgtcaca taaccacagc accgcgcaga aagtgctgca atttacccag cagcagttag
    36541 cgcaggttgg gattaaagcc caggtgactg cgatggatgc cggacagcgg gcggcagaag
    36601 ttgaaggtaa agggcaaaaa gagagcggcg tgcggatgtt ctacactggc tggtcggctt
    36661 caaccggcga agccgactgg gctctatcgc cgctgtttgc ctcgcagaac tggccaccaa
    36721 cgctgtttaa taccgcgttt tacagcaata aacaggtgga tgacttcctg gctcaggcac
    36781 tgaaaactaa tgatccggcg gaaaagaccc gcttgtataa ggcggcgcag gatatcatct
    36841 ggcaagagtc gccgtggatc ccgctggtgg tagaaaaact ggtgtcggca cacagtaaaa
    36901 acctgaccgg tttttggatc atgccagaca ccggcttcag ctttgaagac gcggatttgc
    36961 aataagcaac gcagggagtg gaatgcttaa ttacgttatc aaacgcttac tggggttgat
    37021 tccgacgctg tttatcgtct cggtgctggt gtttttattt gtccacatgc tgcccggcga
    37081 tccggcgcga ttaattgccg ggcccgaagc tgatgcgcag gttatagaac tggtgcgtca
    37141 gcagctgggg ttggatcagc cgctgtatca ccagttctgg cactatatca gcaatgctgt
    37201 gcagggggat tttggcctgt cgatggtgtc gcgtcgtccg gttgccgatg agattgccag
    37261 ccgctttatg ccaacgctgt ggctgaccat aaccagtatg gtctgggcgg tgatatttgg
    37321 tatggcggcg ggaattatcg ccgccgtctg gcgtaaccgt tggccggatc gattgagtat
    37381 gactattgcg gtgtcgggga tctcgtttcc ggcatttgct ctggggatgc ttttaattca
    37441 ggtattctcc gttgaactgg gctggctgcc taccgtggga gcagacagtt ggcagcacta
    37501 cattttaccc tccctgacgc tcggcgcggc agtggccgcc gtgatggcgc gctttacccg
    37561 cgcgtcgttt gtcgacgttt taagcgaaga ttatatgcgt accgcgaggg cgaaaggggt
    37621 gagcgaaacc tgggttgtcc tcaaacacgg gctacgcaac gcgatgatcc cggtagtgac
    37681 catgatgggc ttacagtttg gcttcttgct cggtggttcc atcgtggtgg agaaagtttt
    37741 caactggccc ggacttggac gcttactcgt tgactccgtt gaaatgcgcg attacccggt
    37801 gattcaggcg gaaattctgc ttttctcgct ggaatttatt cttatcaact tagtggtgga
    37861 tgtgctttac gccgccatta acccggctat caggtacaag taaggatgcg actatttaac
    37921 tggcgacgtc aggcggcgtt aaacgccatg ccactggtca aacctgacca ggtacgtaca
    37981 ccgtggcatg aattctggcg acgatttcgc cgtcagcata tggcgatgac cgccgcattg
    38041 ttcgttattt tattgattgt ggtggccatt tttgcacgct ggatcgctcc ctatgacgcc
    38101 gaaaattatt ttgattatga caatctgaat aacggacctt ctttgcagca ctggtttggt
    38161 gtcgattcac tggggcgcga cattttcagc cgtgtcctgg ttggtgcgca aatctcgctg
    38221 gcggcgggcg tgtttgccgt gtttatcggt gcggcgatcg ggacgttgct gggcttgctg
    38281 gctggatatt atgaaggctg gtgggatcgg ctgatcatgc gcatttgcga tgtgttgttt
    38341 gccttcccgg gtattttact ggcgatcgct gttgttgcgg tgttgggaag cggcattgct
    38401 aacgtgatta tcgcagtcgc cattttttcc atccccgcgt ttgcccggct ggtgcgcggc
    38461 aacacgctgg tgttgaaaca gcaaaccttt atcgagtcag cacgcagtat tggtgccagc
    38521 gatatgacca ttttgttgcg tcatatcttg cctgggaccg tctcttctat cgtggtgttt
    38581 ttcaccatgc gcattggcac ctcgattatc tctgccgcca gcctgtcatt tctcggcctc
    38641 ggtgcgcagc cgccgacacc agagtggggg gcaatgctca atgaggcacg agcggatatg
    38701 gttatcgcgc cgcatgtcgc tgtttttccg gccctggcta tttttctgac cgtactggcg
    38761 ttcaatttgt tgggcgatgg tttacgcgat gcgctggatc cgaaaattaa aggatagtta
    38821 cgtttgaata ttgcttgaaa gggtaatcac ctcacaggaa attattgccc taagcaagtg
    38881 ttgtaacttt ctgctgattt tgtagaatcg ggtaatttgg ttaaaaagcc gcagcaaggg
    38941 actattttgc agcggcacag cgttcagaca gttattttgt taaatgtatt aacatgctga
    39001 gtttatacga aaagataaag ataaggctga taattttatt tttattggca gcaatgtcat
    39061 ttattggtct ttttttcatc attaactatc aactggtatc ggaacgcgcg gtaaaacgtg
    39121 ccgatagccg ctttgaactt attcagaaaa acgttggcta tttctttaaa gatattgaac
    39181 gttcggccct gacattaaag gacttactgt atttattaaa aaatacagag gagattcaac
    39241 gcgccgtaat tctgaaaatg gaaatgatgc catttttaga ctcggtggga ctggtacttg
    39301 atgataataa atattatctc ttttcgcgga gggcgaatga taaaatcgtt gtttatcatc
    39361 aggaacaagt aaatggaccg cttgtcgacg agtcagggcg ggttattttt gccgatttta
    39421 acccatcgaa acgaccgtgg tcgatggctt cagatgactc taacaacagc tggaacccgg
    39481 catacaattg ctttgatcgt ccgggtaaaa aatgtatctc ttttacgcta cacatcaacg
    39541 gcaaagatca tgatttgtta gcagtagata aaattcatgt cgatttaaac tggcgatatc
    39601 tgaacgagta tcttgatcac atcagcgcta atgatgaagt tctattttta aagcaaggtc
    39661 atgagatcat tgctaagaat caactcgctc gtgaaaaatt gattatttat aatagcgaag
    39721 gtaattataa tattattgat tctgtcgata cggaatatat tgaaaaaaca tcagcggtgc
    39781 caaacaacgc attattcgaa atctattttt attatcctgg tggtaattta ttgaacgcat
    39841 cagataaact tttttatctg ccgtttgctt tcattattat cgtattgctg gtggtttatt
    39901 taatgaccac tcgagtgttc cgtcggcaat tttctgaaat gacagatttg gtcaatacgc
    39961 tggcgttttt gcccgactct acggatcaaa tcgaggctct gaagattcgt gaaggcgatg
    40021 cgaaagagat tatcagcatc aaaaattcga tcgcggaaat gaaagatgct gaaattgaac
    40081 gggcaaataa attgctctcg ctgatctctt acgatcagga aagcggtttt attaaaaata
    40141 tggcgattat tgagtccaac aataatcagt atctggctgt ggggatcatc aaactgtgtg
    40201 gtctggaagc tgtggaagcg gtgtttggtg ttgatgaacg caataaaatc gtcaggaaat
    40261 tgtgtcagcg aattgccgag aaatatgcgc aatgctgcga tatcgtgaca tttaatgccg
    40321 atctctattt actcctgtgc cgggaaaatg tacagacgtt tacccgtaag atagcgacgg
    40381 taaacgattt tgacagcagt tttggctacc gcaatctgcg catccataag tctgctattt
    40441 gtgaaccttt gcagggggaa aacgcctgga gttacgcaga aaagctgaaa ctggcgattt
    40501 ccagtatccg caaccatatg ttctcagagt ttattttctg tgatgatgcg aaactcaacg
    40561 aaatagaaga gaatatctgg attgcgcgta atattcgcca tgcaatggaa attggcgaac
    40621 tattcctggt ctatcaaccg attgttgata ttaacacccg caccattttg ggcgcggagg
    40681 cgttgtgccg ttgggtgtct gcggagcggg ggattatttc accgctgaag ttcattacca
    40741 ttgctgaaga tatcgggttt atcaatgagc tgggctatca aattattaaa accgcgatgg
    40801 gtgaattcag acattttagt cagcgtgcgt cgctgaagga tgatttctta ctgcatatta
    40861 atgtttcgcc ctggcagtta aacgaaccac actttcatga gcgttttact accatcatga
    40921 aagaaaatgg cctgaaggcg aacagccttt gtgttgagat caccgaaacc gtgatcgagc
    40981 gaattaatga acatttctat ctcaatattg aacaactgcg taaacaaggg gtacgggtat
    41041 cgattgatga ctttggcacc ggtttgtcaa acctgaaacg tttttatgaa attaatccag
    41101 acagtataaa ggtggattcg caatttaccg gcgatatttt cggtactgcg gtaaaaattg
    41161 tgcgcattat tttcgatctg gcacgctata accggatccc ggtgattgcg gaaggcgtag
    41221 agagcgaaga cgttgcgcgt gaattaatca aattaggatg tgttcaggct caggggtatc
    41281 tgtaccagaa acccatgcca ttctccgcct gggataaaag tggaaaatta gtaaaagagt
    41341 agtttacgta tgtccagaat caataaattc gtacttacag tcagtctgct gatttttatc
    41401 atgatttcag cagttgcctg cgggatctac actcaaatgg taaaggaacg ggtgtatggc
    41461 ctgaaacagt ccgttattga tactgctttt gcggtggcaa atattgctga atatcggcgt
    41521 agtgtggcaa ttgatctcat caacacgcta aatcctacgg aggaacagct gttggttggc
    41581 ttacgcacag cttacgccga ctcggtttca ccctcttatt tgtacgatgt cggtccttat
    41641 ctgatttcca gtgacgaatg tattcaggta aaggagttcg agaaaaatta ttgtgcagat
    41701 attatgcagg ttgttaagta tcgacatgtc aaaaatacag ggtttatctc ttttgatggt
    41761 aaaaccttcg tctattacct ctatccggta actcacaatc gtagtctgat atttttgctt
    41821 ggtctggaac gtttttcttt actgtcaaaa tcgctggcga tggatagcga gaacctgatg
    41881 ttctccctgt tcaagaacgg taaatcggtg accggtgatg aatataacgc taaaaacgcc
    41941 atcttcaccg tttcggaagc aatggagcac ttctcctatt tgccgaccgg gttgtacgta
    42001 tttgcttata aaaaagacgt ttattttcag gtatgtacat tgattatttt ctttgccgca
    42061 ttggtggcag tgatatcggg tgccagttgc ctctatctgg tacgcagagt gattaatcgt
    42121 ggtattgtgg agaaagaagc cattattaat aaccattttg aacgcgtact ggatggcggg
    42181 cttttctttt cggctgccga tgtcaaaaaa ctctacagta tgtataactc ggcattcctg
    42241 gacgatctga ccaaagcgat gggcagaaaa tcttttgacg aagatttaaa agcgctgccg
    42301 gaaaaaggcg gttatttgtg cctgtttgac gtcgataaat tcaaaaacat taacgacacc
    42361 ttcggtcatt tgctgggcga tgaagtgttg atgaaagtgg tgaaaatcct taaatcacag
    42421 atcccggtag ataaaggtaa agtttaccgc ttcggaggtg acgaatttgc ggtgatttat
    42481 actggcggca cgctggaaga gttgctatcg attctaaaag aaatcgttca tttccaggtg
    42541 ggaagcatta atttaagcac cagtatcggt gtagcgcatt caaatgaatg tactaccgtt
    42601 gaacgtttga aaatgctggc ggatgagcgg ctgtataaga gtaagaaaaa cggcagggcg
    42661 cagattagct ggcagtaatc attattcgca ggccggacaa atgattttgc ccggcctgaa
    42721 ttaattaaac ccggctaccc cacaaatcgt actcatcggc gtgctcgact ttcactcgca
    42781 ggatatcacc cggcttaacg ttggtttcac cattgagata aaccgcgccg tcgatttccg
    42841 gtgcatctgc catgctgcga ccaatcgcgc cttcttcgtc cacttcgtcg ataatcacca
    42901 gaatttcacg gcccactttc tcttgcaggc gctcggcgga aatttgctgc tgcaactgca
    42961 tgaaacggtt ccagcgttct tctttcactt cttccggaac ctggtcaggc agggcattgg
    43021 cgtctgcacc ttcaaccggg ctgtatttaa agcagccaac gcgatccaga cgtgcttctt
    43081 tcaggaagtc gagtagcatc tggaaatctt cttccgtctc gccagggaag ccaacaataa
    43141 aggttgagcg tagggtcagt tccgggcaga tttcgcgcca ctgtttgatg cgcgccagtt
    43201 ggcgatctac agaacccgga cgcttcatca gtttgagaat gcgcgggctg gcgtgctgca
    43261 acggaatgtc cagatacggc aggattttgc cctctgccat cagtgggatg acgtcgtcca
    43321 catgcggata agggtaaacg tagtgcagac gtgtccagat ccccagtttc gataactgtt
    43381 cgcacaggct gaccatgctg gtttttaccg gctcgccgtt gtggaagcca gtacgatgtt
    43441 taacatcgac gccatacgcg gaagtatcct gcgagatcac cagaatttct ttaacgcccg
    43501 catcaaccag acgtttcgct tcacttaaca cttcgccaat cggacggctc accagatcgc
    43561 cgcgcataga cggaataatg cagaaggtgc agcggtgatt acagccttca gaaattttca
    43621 gataggcata atgacgcggc gtcagtttca caccttgttc tggcaccagg ctcaggaatg
    43681 ggttgtgttt cggttttggc acgtagtgat gaacgtgctc cagaacctgc tcatagctat
    43741 gaggcccggt gatttccagc actttcgggt ggacttcgcg gatctgatct tcttttgccc
    43801 ccagacaacc ggtcacaatt accttgccgt tttcattcaa cgcttcacca atggcttcca
    43861 gtgattcttg taccgcgctg tcaataaagc cgcaggtgtt gacgatcacc atgtccgcat
    43921 catcatagct cggtaccacg tcataacctt cagtgcgtag ttcggtgaga atacgctctg
    43981 aatcgacaag gtttttcgga cagccaaggg aaacaaagcc gattttcggc tggggagtta
    44041 ctttgctcat agcttaaaaa atattcagtt acaggaaagg tcagggcagg gattctacag
    44101 agttctggat aaaatttgta tcgcaatctc attcgctggc ggagttgaag gaaatgtaaa
    44161 ttttgttaat tcggcgtgaa gaattgatcc tggacagcat tttgctcaaa aaacagccat
    44221 actatttagt tgcaacaagg ctggaaagag gaggatcgaa gtatgttcgt tgacagacag
    44281 cgaatcgata tgctgaaccg gttgatcgac gcacgcattg acctcgccgc atacgtgcaa
    44341 ctgaggaagg caaaaggata catgtccgtc agcgaaagca atcatctacg agataacttt
    44401 tttaaactga atcgcgaact gcacgataaa tcgctgcggt tgaatcttca tctggatcag
    44461 gaagaatgga gtgctcttca tcatgctgaa gaagcattag cgacagccgc agtatgtttg
    44521 atgagtgggc accatgattg cccgactgtt attaccgtca acgccgataa gcttgaaaat
    44581 tgtctgatga gcttaacgct gagtatccag agcctgcaga agcacgccat gcttgagaag
    44641 gcctgaaaac taagggggag aaagcgtctc ccccttcatg tttaagtttt tgtaaaaatg
    44701 aatttgttat ctcctccact gactacgctt taagccagag tcaatccgga ggcgttatgc
    44761 atcgacaatc ctttttcctt gtgcccctta tttgtctttc ttccgctctc tgggcggctc
    44821 ctgcaacggt aaatgtcgaa gtactgcaag acaaactcga ccatccctgg gcgctggcct
    44881 ttttacccga taatcacggt atgttaatca ctctgcgcgg cggcgagttg cgtcactggc
    44941 aagcaggaaa aggattatct gcgccgcttt ccggagtgcc ggacgtttgg gcgcacgggc
    45001 agggcggcct gctggacgtg gttttagcgc ccgattttgc tcagtcgcgc cgcatctggt
    45061 taagttattc cgaagttggc gatgatggca aagccggaac tgctgtgggt tatggccgct
    45121 taagtgatga tctctcaaaa gtgaccgact tccgcaccgt ctttcgtcag atgccaaaac
    45181 tgtctaccgg caaccatttt ggcgggcgga tggtattcga cggtaaaggt tatcttttta
    45241 ttgctctggg cgaaaacaat cagcgcccga cggcgcagga tctggataaa ttacagggca
    45301 aactggtgcg tctgaccgac ctgggagaaa tcccggatga taatcctttt ataaaggaat
    45361 ccggtgtgcg cgccgagatc tggtcttatg gcattcgtaa tccgcaagga atggcgatga
    45421 atccgtggag taatgcacta tggctgaatg aacatggccc gcgcggtggt gatgaaatta
    45481 atattccgca aaaaggcaaa aactacggct ggccgctggc aacctgggga atcaactatt
    45541 cgggctttaa gataccggaa gcgaaagggg agatcgtcgc cgggaccgag caacctgttt
    45601 tttactggaa agattcgccc gccgtgagcg gcatggcctt ctataacagc gacaaattcc
    45661 cccagtggca gcaaaaatta tttattggcg cgctgaaaga taaagatgtc attgtgatga
    45721 gcgtcaacgg cgacaaagtg acagaagatg gccgtatttt aacggacaga gggcagcgaa
    45781 ttcgtgatgt tcgcactgga cccgacggtt atttatacgt tctcaccgac gagtccagtg
    45841 gggaattact taaagttagc ccacgcaatt agctaacggg aatcatcacc actttgcgca
    45901 ctgcggggcg ttcagtgagt tgctgatacc agcgttgcag atttggacgc ggtgtccagg
    45961 tcaggccaac gttaaacaaa ttgtagatga acggtgcgat agcgatatcg cccacaccaa
    46021 actcgtcgcc tgagaaccat tttacttttg ccagttccgc atcgagcagg gcaaacaggg
    46081 cgtcgcactc tttgcaactg gcatcaatgg cggcctgatc gcgctcttcc ggtggtgttc
    46141 tgactaatcc catcaggatc ccgcgatgag cattactgag cgtctggttt gcccagtcca
    46201 tccatttttc tgcttccgca cgacgtgccg gtgagtcgat ccacaggcgt ttttgcccgt
    46261 actgtgccgc cagatagcgg acaatggcgt ttgattccca aagaatgaga tcactttcgt
    46321 cgtcacgcaa caacggcacc agcccgttag ggttcatcgc cagaaaatca gcatcgtgat
    46381 ttatcccaaa ctcacggccc gcgagaattt gctcataagg tagttccagt tcttcgagcg
    46441 tcagcaatac tttttttacg ttggttgaat tattccgacc ccacagcgta atcatactca
    46501 ccccttttcc gacaggcagc cacaggctga acaggactcc gatggtgaag taaagttaac
    46561 tttaagcaac agctggcaaa aaaatgacac cagaagcagc gaagcggcag gatattgcat
    46621 aaactttaaa aactttacca acttacggtt tctttaagtt tgtgtgtgcg ttattaatca
    46681 ccgaacttat catacggcga tataacgtat tttttttgaa tggatactcg ggtggcattt
    46741 atgacgcaat actcctctct ccttcgtggt cttgcagcgg gttctgcatt tttattcctt
    46801 tttgccccaa cggcattcgc ggcggaacaa accgttgaag cgccgagcgt ggatgcgcgt
    46861 gcatggattt taatggatta cgccagcggt aaagtgctgg cagaaggcaa tgcggatgag
    46921 aaactggatc ccgcgagcct gactaaaatt atgaccagct atgtggttgg gcaggcgctt
    46981 aaggccgata agattaaact caccgatatg gtgacggtcg gtaaagatgc ctgggcgacg
    47041 ggaaatccgg cactgcgtgg ttcatcggta atgttcctca aaccgggcga tcaggtttcg
    47101 gtggcagact tgaacaaagg tgtgattatc cagtccggta atgacgcctg tattgcgctg
    47161 gctgattacg ttgccgggag ccaggagtca tttattggtt tgatgaatgg ttatgccaaa
    47221 aaactgggtc tgaccaacac taccttccag acggtgcacg gcctggatgc gccggggcag
    47281 ttcagtaccg cgcgcgatat ggcattgctg ggtaaagcat tgatccacga tgtgccggaa
    47341 gagtacgcca ttcataaaga gaaagagttc accttcaaca aaattcgtca gcctaaccgt
    47401 aatcgtctgc tgtggagcag caatctgaat gttgatggca tgaagacagg aaccacggca
    47461 ggcgcgggat ataatctggt tgcttcggct acccagggcg atatgcgttt aatctccgta
    47521 gtgctggggg cgaaaactga ccgtatccgt tttaatgagt ctgagaaatt attgacctgg
    47581 ggcttccgtt tctttgaaac cgtgacgcca attaaacctg atgccacatt tgtgactcag
    47641 cgcgtctggt ttggtgataa gagcgaagtg aatctcgggg caggcgaagc gggctcagtg
    47701 accataccgc gtgggcagct gaaaaacctg aaggcgagtt atacgttaac ggaaccgcag
    47761 cttaccgcac cgctgaaaaa aggtcaggtt gtcggcacca ttgatttcca gcttaacggt
    47821 aaatccattg agcagcgtcc gctgatcgtg atggaaaatg tggaagaggg cggattcttt
    47881 ggtcggatgt gggatttcgt gatgatgaaa ttccatcagt ggttcggcag ctggttctct
    47941 taatcttctg ataaccggat ggcgcgaaac gtcatccggt tatacgtcat taatacatca
    48001 acttaatacg ctgcgtctgc gcgtacttca catactcatc ttccgggcaa caatcgctca
    48061 caacaatatc aaagcgtttc aggtcaccca tgcgcgccgg acgcaccttg ccaaatttac
    48121 tgtggtcgac aaccagcaca tgcttttgcg ccatcgacat ggcccagtgt tttaccggca
    48181 actcttcaag attaaagcag gtagcgcctt tactgacatg cacgcccgca gcggaataaa
    48241 aagcgatatc cgggcaaaaa ttattcagcg tttgctgaaa atcgatgggt ttgaaaatgg
    48301 cgttgctggc gtgaaattca ccaccgcaaa gaaacgcgcg gcaatggggt ttctctttca
    48361 gcgccagaaa ggtatttagc gaataacaaa cggcggtaaa agggatttca ttatcaattg
    48421 cttcaataat ccacggcgtg gtggtgccac agtcaaaaaa gagggtctga tcgggttcta
    48481 ccagcgtcgc agccagtttt gccgcccggc gtttttcttc caccaggcgg gatttttgat
    48541 cgcttaacag gtaatggctg gcgctgcgtg gttccagaac aatatagccg ccgagcaaaa
    48601 cgacgggcgc actgtggttg ttcagatcgc gacgaatcgt catctccgaa accccaagca
    48661 gggcggcggc gtctttaaga tgtaacttat cactgcgttt taattcttgc agcagctgcc
    48721 cgatacgctc ttcgcgacgt gtttccataa tccctctgaa tagttattga agcgagccgc
    48781 tcaatactac actttttagc agagatcagt cacgcaccca gcctttgcgg atcggtaatg
    48841 caaaacagaa gcgataccac gattgcagac gttgatagag tttatgcccc atcgcttgcc
    48901 agataatctg ggcgctaagg cagccaatca tacctgccag caatccaccg agcatatcca
    48961 gcggccagtg gacgccaaga taaacgcgcg accaggcaat gacgacggcc agcaccatta
    49021 aaagtgagcc ggaccacagg cgatgccagc ataaaaatgc cagtgcaaag gtgaaaatca
    49081 ccgtaccgtg atcgctcggg aatgagtcat ccgccgcatg atgcaggaag ttatagccga
    49141 tattttcgac aaagggtcgg tcgtgcggaa aaagatgtcc catcgtccag gacacaaaca
    49201 ggctgaccgc cagcgcgata gcgattttta tcaccagttg ccgttgtgct gtaagccccc
    49261 acaaccaaag tactgcggcc agcaacggca ccacggtaat caaatcttta gcaataaaaa
    49321 tcgccaacga gatcatccac ggcgcagagt ctggcgttgc gttaataaga gagaatagag
    49381 agagattcaa attttccagc ataacttccc gacgcaaagt gattaaaagg gagccaatac
    49441 aggcaagtcg ttgagaataa agtgcaggtt aaactgggta aagcggcatc gtcttatttc
    49501 cctcaagcgg cctgtttacg gtaggtgatt gtaacgggca aaggttaaat aaaacttaaa
    49561 gaaagcgtag ctatactcgc aataatgtaa gaatgtgctt aaccgtggtt tcagctacaa
    49621 aattcgcttt ctcgttagct gcgcttttat taaactttgc gcgattatta ttggcgaaga
    49681 aattgcatgc aaaataaatt agcttccggt gccaggcttg gacgtcaggc gttacttttc
    49741 cctctctgtc tggtgcttta cgaattttca acctatatcg gcaacgatat gattcaaccc
    49801 ggtatgttgg ccgtggtgga acaatatcag gcgggcattg attgggttcc tacttcgatg
    49861 actgcgtatc tggcgggcgg gatgttttta caatggctcc tggggccgtt gtcggatcgt
    49921 attggccgcc gtccggtgat gttggcggga gtggtgtggt ttatcgtcac ctgtctggca
    49981 atattactgg cgcaaaacat tgaacaattc accctgttgc gcttcttgca gggcataagc
    50041 ctctgtttca ttggcgctgt gggatacgcc gcaattcagg aatccttcga agaggccgtt
    50101 tgtatcaaga tcaccgcgct aatggcgaac gtggcgctga ttgctccact acttggtccg
    50161 ctggtgggcg cggcgtggat ccatgtgctg ccctgggaag ggatgtttgt cttgtttgcc
    50221 gcattggcag cgatctcctt tttcggtctg caacgagcta tgcctgaaac cgccacgcgt
    50281 ataggcgaga aactgtcgct gaaagaactc ggtcgtgact ataagctggt gctgaagaac
    50341 ggccgctttg tggcgggggc gctggcgctg ggattcgtta gcctgccgtt gttggcgtgg
    50401 atcgcccagt cgccgattat cattattacc ggtgagcagt tgagcagcta tgaatatggc
    50461 ttgctgcaag tgcctatttt cggggcgtta attgcgggta acttgctgtt agcgcgtctg
    50521 acctcgcgcc gcaccgtacg ttcgctgatt attatgggtg gctggccgat tatgattggt
    50581 ctgttggtcg ctgctgcggc aacggttatc tcatcgcacg cgtatttatg gatgaccgcc
    50641 gggttaagta tttatgcttt cggtattggt ctggcgaatg cgggactggt gcgattaacc
    50701 ctgtttgcca gcgatatgag taaaggtacg gtttctgcgg cgatggggat gctgcaaatg
    50761 ctgatcttta ccgtcggtat tgaaatcagc aaacatgcct ggctgaacgg gggcaacgga
    50821 ctgtttaatc tcttcaacct tgtcaacggc attttgtggc tgtcgctgat ggttatcttt
    50881 ttaaaagata aacagatggg aaattctcac gaagggtaaa aaaatgcctg attgctttgt
    50941 gcgatcaggc attctcgaat taatggtgat ggtcgtcaat ctggtgttcg ataaccatcc
    51001 cttcacctac gctggcaaga tggcgaacat aaggatgcgg gcggtaagcc ggagctggcg
    51061 caggagccac atagacggtt tgcggcacag tcgcgacgct gaccgcttgt ggaacgctga
    51121 ctgaatcagg aaccacaacc actttgtatc cactcggcac atcgacggtg acactttgcg
    51181 ccatcgccac accagtaaag cccattaaaa gcgcgcccaa cagtagacaa tttttcataa
    51241 tcatttcgcc tgaaataatg cagcgggtta atttccgcga attatgcagc cttcattcag
    51301 gcattgtgtt gccgggcgtg ctgccttttt tattgcttaa atggcgcttc gtgtttaagc
    51361 actttatcga tcacatccag tacgccttca cggttattgg agcctgcccg gtattttgcc
    51421 gctgcgacga ccgcgctgcc ggcattttcc attgcaaaac taaagcctgc ctgacgcagc
    51481 atctcaatat cgttaccgcc atcgccaaag accaccactt cgctgtcgtc tattccccat
    51541 aatttctgca gttggcgaag gccattggct ttatgtacgc cggggataat cagatcgatg
    51601 ctgccgttgc cggtgtggac cggcaccata atatcgccga tggcctcatg taatgctttt
    51661 tgtacttgtg gaatcagttc atcggaaaga ttcagaccaa acttaaagaa gatatcttct
    51721 aagttgtcaa agttatcgac gtattccaga cggtgataat acatttccgc caccgttttc
    51781 atggcatcgt catatttttt gagggtatag gcgctatttt ttccgcaggc aataatttcc
    51841 acttccgggc gcgtcagcaa atgttccacg acagtagcaa acgcatcctt cgacagctcg
    51901 ccattaaaaa catctttgcc ttcgctcact acccagccgc cgttttcagc cacaaaggct
    51961 atttcattag caatttcagg gaagaaagag atcaactgat aatattgatt cccgctggcg
    52021 accacaaagc gaatgccttg tgctttcatt tgctgatact gagccataaa ccgctcacgg
    52081 ttataggttt tttgatcgct taagaaagta ccatccatgt ctaccgcaat taatttaatg
    52141 ctcatcaact attctccatc gccgtctgcg ttttggtatc gggtttggcg acggctttcg
    52201 cgacaatagc cgcgagaata accagcgcca gtacaaccag cattgcacta cgtaatccat
    52261 aatgttcgcc gagatagccc agcagcggcg gcccgacgag gaaagccaga taaccggtcg
    52321 tagctaccac gctgacgcgg gttggtgcat cggggccggt atcgctggcg gcagaaatag
    52381 tcagcgggaa gcccaacgag gcacccagtc cccaaagtac aacagacacc ccagcgaccc
    52441 aggcgctatc gacaaaaata atcagcccaa tacccaacgc ccccattagt gcactggccc
    52501 gaaccacggc aacgcgactg taacggtcaa tgaaccaacc gccggtaaag cgtccaacgg
    52561 tcatccccag ggtaaacccg gcataaatca gcgagccgga agtagggcta aaaccgtgac
    52621 catcaaccat taataagggt aaccagtcgt tggcagaacc ttcggcaaag gccatcgcca
    52681 gcaccacaac accaatcagc agcaactgga tatcgcgata aaaaggtacg cctttttcac
    52741 catgctgggt gccatcggca gcatttttgc ccgtaccgtc agggattgcc tgaatggcga
    52801 tataaatagg cgcgatgcct accagcgccg ccaataaaat gtgcaccgtt gccggaacgc
    52861 caaaggccgt cagtgccatc ccgacacctg cgcctgccag cgtgcccagg ctataaaaac
    52921 cgtgcatcat cggtaaaacc gtcttattca tttctctctc aacggcggca ccttcaacgt
    52981 ttatcgccac ttccgcagaa ccaaaactag ccccaaagac gccgagacca acggcaaaga
    53041 gcaagggcga tgtcagccag agtgccagac ttagtatcat catcccaatc aatgcgcagg
    53101 acatcgtgac aaggatgaca ttacgcgtcc caaagcgttt cactaaccac gctgagcaga
    53161 gaataccgct catcgaaccg atcgacagac caaagagaac accgcccatt tcagcgatcg
    53221 agacagagag aatatcgcgg atagcaggcg tacgggttgc ccaggacgcc attaacaggc
    53281 ctggcaaaaa gaagaacata aacagcgccc aggttcggcg tttcaatgca ttacgtgaag
    53341 aatttacggt catagaccac gtcaaaataa gaagagggaa gacaacatta gcaagactgt
    53401 gtacatttgt acacaattgc agagagagga aatgacatgc gtcgcgctaa cgatccgcaa
    53461 cggcgagaaa aaattatcca ggccacgctg gaggcggtga aactttacgg aatacatgct
    53521 gttacacacc gcaaaattgc tgcccttgcc ggggtgccgt tgggatcgat gacctactat
    53581 ttttcaggaa ttgatgagtt gttgctggag gcgttcagcc gttttactga gatcatgtcc
    53641 cggcaatatc aggcattttt tagcgatgtt agtgatgctc caggcgcatg ccaggctatc
    53701 accgatatga tctacagctc acaggtcgca acgccggata atatggagct gatgtaccag
    53761 ctctacgcgc tggctagccg aaaaccgctc ttaaaaacgg taatgcaaaa ctggatgcag
    53821 cgcagtcaac aaacgctcga acaatggttt gaacccggaa ccgcccgcgc gcttgatgcg
    53881 tttattgagg ggatgacgct gcattttgtc accgaccgta agccgctatc acgcgaggag
    53941 attttgagga tggttgagag gattgctggg tagtagataa atttcaggca acaaaaaacc
    54001 catttatgta aacgggttaa tgaaaacaat aagttagagt aaaaacaata agttggattg
    54061 gtggtcagtg gtggttatta cggggtaatg ccaaccgctg ccgccacttt gtcgccattt
    54121 ggcagcgttg ccagaggatt gaaacggagc gccgtttcca gatgatccgg tgccagatgt
    54181 gcgtaacgca tagtcatttt tatatcgtgg tgtccaagaa ttttttgtaa ggccagaatg
    54241 tttccacccg acatcatgaa gtgcgccgca aacgtatggc gcagaacgtg tgtcagttga
    54301 ccgcgaggga gcacgataga cgttttttcc atcacggata aaaattgaaa atagcagtct
    54361 gtgaagaaat tgaacccatc aagcgccatg atctcttcgt aaagctcttt actgataggg
    54421 atgcttctgt ttttcttccc cttcgttctg acaaaggtaa ttcggtattt ggtcacctgt
    54481 gagcgggtaa gatttacggc ttcacgccag cgtgcgcctg tgcttaagca tatcttaact
    54541 accagtgcca gaattgggtc ctgacgtttg caatcagcca gcaattcaac aatctgctca
    54601 tgggtaagcc atgccatctc tttttctgcg atggtgaatt ttcgcatgtt ctccagtggg
    54661 ttcggatacg accattcgcc cagacgggat agttcgctaa aaacactact tagatagctt
    54721 tgctccaggt taatggtgac cgggcttgct cctttcttcc atttctcgct gaagtagatc
    54781 tcgcctgtca ggcgtttatc tcgatagtgg gcaaacattt tagaggtgag atcggttgca
    54841 agaggattgc ccagagcatc aaccatcaac agcaatttgt catagacatg ctgcccagcg
    54901 gttagagatt taccatgtag tttgaaccat agctcaacca cgtctttcaa tgtccgacga
    54961 tccactgatt cgcccagcca gggctttgct tcggtttctt ccatcgtgtg acgctcaaaa
    55021 gccagagctt cgcctttggt ggcgaattgt ttacgcacac ggcgtccact acgtccggcg
    55081 gggtaacatt cgcaaagcca ttttcctgtg gtgagttttc gtactgccat aaaaaatgcc
    55141 ctccaataga gagcattttt actgtatgta taaccagtgt caatgtatga aatcctgcga
    55201 ccatacatct cactgaagcc ataatgaagt tggctatttt ttgctatgtg agtatgtgac
    55261 ttttgcggtt agcctgcggc tcattgttat attaggcgca gatataaaag caaaatttat
    55321 cgcgagtttt tagtacagat tttttttgat ttactaatag ttccatcatt gcaaacgaac
    55381 tttccatcgg aggtacagtg agagacacct ccctttttcc cagaacaagg ataattttta
    55441 gcataggtag ttagtgggtt taataacaaa gagcatgata aaaccacaaa aaatacctta
    55501 ccaagcatag tttcctcccg gtattaccta acgtacttaa ttgttaaact tataattttc
    55561 ccaatcattt caacatcttc tatcttgcac tcgaaggctc tgtttccacc ttcaacgaag
    55621 attcttccac cgggtaaacg agtaatgtcg cggattgtta tttcgccatc aatacttatt
    55681 acccatttac cgtcacgtat atcatcaaat tctttatcac aaataaattc agaattgtta
    55741 tctgtgatga caaaaggttt tttaaacgta gagggtagaa atcccttatc aaaaatataa
    55801 aaaccgtctt cacgcaaggc accatcagat aatacatatt tagcaacttc catagtattt
    55861 gtattacctg aagtttgctt tgaaccatgt ccggttgtga gccaattaag cgaggtgccc
    55921 gtttcaaggg cgcactggat tacccattct gctgggaatg agtcacgcat gtagcgtgtg
    55981 gcgagtgtac ttttagagat tcctaaatga tcgcacaacg cctgtcgagt tttgaatcca
    56041 taagcttcta ccatgcgctc tatagcgcct cgtccgcctt tctccaaatt catggtcact
    56101 ccaagtgaac ttttatcttg acgatttcac tgtgcgatcg tatgtttatg gtgttcacaa
    56161 aatacaaacg atccgtattc gtcctgatta atcatcatta aacgaggaat gttgcatcat
    56221 gagacctaac atttcaatca ctcttaccac gcctcatgtg actattgaac gctatagcga
    56281 gctgacaggg ctgtccatcg ataccatcaa tgacatgttg gctgacggac gccttatccg
    56341 tcaccgtctg cgcaaagata aaaaacgtga aaaagtgatg atcaacatag cagccatgac
    56401 cgttgatgcg ctttcagaat gcaatctaaa ccttaattag ttcgattctg aaatacatca
    56461 gaggcattga ccatgtttga ttaccaagtt tccaaacatc cacattttga tgaagcctgt
    56521 cgtgcattcg cattgcgcca caacctagtg caactggcag aacgtgcagg catgaatgtg
    56581 cagattctgc ggaacaagct gaacccagct caacctcatt tattaaccgc accagaaatc
    56641 tggttgctta ccgatctgac tgaagattca acgctggtag atggctttct ggcacagatt
    56701 cactgcctgc catgtgtacc gattaatgag gtagcaaaag agaaactgcc gcattacgtc
    56761 atgagtgcaa ctgcagagat agggcgtgtc gcggcaggtg cggtttctgg cgatgtaaaa
    56821 accagtgcag gccgtcgtga tgcgatcagc agcattaact ctgttacacg actgatggcg
    56881 ctggctgctg tttcattgca ggcccgttta caggctaatc ctgcgatggc gagtgcagtt
    56941 gataccgtga ctggcctcgg tgcttcattc ggtttgctgt gaggtgctta tgctgacgaa
    57001 cgaaccatca tttgcatcgc tgctggtaaa acaaagtccg gcaatgcact acggtcacgg
    57061 ctggatcatg ggtgaggatg gtaaacgctg gcatccgtgc cgttcacaag atgaattgct
    57121 ggcagaacta tctacgaaaa aacgggggaa caaatggcta ttgaaggcgc tgcggcgact
    57181 gttccattaa gacccggtga acgcctgaat ggacttaatc atattgcgga gttaagggcg
    57241 aaagtttttg gtttgaatat tgagtcagag cttgagcggt ttattaaaga tatgcgtgat
    57301 cctcgggata tcaataatga acaaaataaa cgggcactag ctgccatatt ctttatggca
    57361 aaaattccag ctgaacgtca tagcatcagc attaatgagc tgaccactga cgaaaagcgg
    57421 gagttgatta aagcaatgaa tcattttcgt gcagtggtga gcttatttcc cagacggcta
    57481 accatgccga attaaccaac taatgaaatt aatggcgtaa acccgccggg catcccttta
    57541 tctaaattca ggagaattgc ttatgcgtaa tattgaaacc ctcacgacca aaaccggacc
    57601 ggatgatgca ggtcttaatc ttttactgac agaggctcgt ctggaagaac gccgggcaag
    57661 ggctgaagca atggctgctc gcctcgatag cctggcgtgt catatcacct cccgccagct
    57721 aaaccacgtc gaagcggcag aactgctgcg tgtgactgct gaagcaatcc agaacgaagc
    57781 gcaggagatc cactgatggc tgatgcaatg gatctcgtac agcagcgcgt tgaagaagaa
    57841 cgccaacgcc atatccgtgc tgcccgtgcc aaaacgccgg gcgtgtcccg cgtgctttgc
    57901 gttgagtgtg aagcgccaat tccgccagca cgccgccgtg ccattccggg tgtgcagctt
    57961 tgcattacct gtcaggaaat cgcagagctg aaaggcaaac attacaacgg aggtgctgta
    58021 tgagcaccat cctgaaatgg gcaggaaata aaaccgccat aatgtccgaa ctgaaaaagc
    58081 atcttcctgc tggcccgcga ctggttgaac ctttcgcggg ttcctgtgca gtgatgatgg
    58141 agacggatta ccccagctat cttgttgcgg atattaatcc tgatttaatc aacctctata
    58201 aaaaggttgc tgctgattgt gaggcgttta tatctcgtgc cagagcttta tttgaggaag
    58261 caaacaggga ggtagcttat tacaacataa ggcaggagtt taattactcc actgaaatta
    58321 ctgatttcat gaaagcggta tatttcctgt atctcaatcg tcacggttac cgtggtttat
    58381 gtcgctataa caagagcgga catttcaaca ttccctacgg taattataaa aatccgtatt
    58441 tccctgaaaa agaaattcgc gcatttgcag agaaagccca gcgggcaacg tttatctgcg
    58501 ccagctttga tgaaacgctg gcgatgttga aggtggggga tgtggtgtat tgcgatccgc
    58561 cttatgacgg tacgttttcc ggctatcaca ctgatggttt cactgaagat gatcagtatc
    58621 acctggcatc cattcttgaa catcggtcat cagaaggaca tccggtcatt gtttctaaca
    58681 gtaacacgtc tttgacccgg tccctttatc gtaatttcac tcaccactac atcagggcga
    58741 aacgcagcat cggcgttgca gcgggggagg gaaaatttgc aacagagatg attgccacta
    58801 aatctgctaa ttggtttagt gccgatttta gtaggggacg tgactctact gttattttcg
    58861 gggtgcaagt gtgaaagaaa tgcaccacgg aattcatcat ttccatggga cgcctgtctg
    58921 gggaagtgct ggcgacgttc atcgtattgc ggtgagcgga gctggcgctt tcgtctccta
    58981 tgtacgacca gatcagattg cggcgtccat tcagcacgct caggtcgtcg gcattgataa
    59041 cggcgcattt tctgcatggg tgcgtgggct aaaaattaac tggagtgatt tttataaatg
    59101 gctcctgaac tattaccacc atcctaaggt cgcttttttt gtcattcctg atgttgtgga
    59161 cggaggtgaa cgtgacaatg atgccctgat aaacgaagtt ccgaaaatgt tctacgggaa
    59221 ggcaactccc gtctggcatc tgcacgagtc aatcgatcgg cttatcgagc tatgtcgtga
    59281 atggcctcgt gtctgctttg gatcgtctgg tgaatatgcg gctatcagaa ctgcgcactg
    59341 gcatcgtcgt atgcaggacg cttttgaagc aatttattgc cgacacaatt tcaaaacagc
    59401 tgttcatggt ttgcgcatgc ttgacggtcg tgtgttggga aattacccac tggcgactgc
    59461 cgacagtaca aatcttgcct gcaatgtccc caaatttaat agcaaatatc ctgagctgac
    59521 gcgggctatt caggaggctg agtattcgcg caatctgacg gaaaaggagc tgaaagctgt
    59581 cattctgaaa aaccgttgcg caattttaaa aggtgcaatt gaagctgttc gcccaccttc
    59641 agtttctgat tggctgtcga atggtttgca gccttcacag ctcgaactgg agattgcgta
    59701 atgaactaca gctattcctg gaatgctgag aaaaaagcaa tcaatcctta cgtagagaca
    59761 gaagagcaat cttcagtttc tgcgctttca aacctgatcg ctctgtacgc tgccgataac
    59821 gagcaggaac agctgcgccg cgaggcactg agtgatcagg tctgggagcg ttatttcttt
    59881 aatgaatccc gtgatcctgt ccagcgcgaa atggagcagg ataagctcat tagtcgggca
    59941 aagctggcgc atgagcagca gcgttttaat ccagatatgg tcattctggc ggacgtcaat
    60001 gcccagcctt cccatatcag caagccgctg atgcaacgta ttgaatactt cagcagcctg
    60061 ggcaggccaa aggcttattc ccgctatttg cgtgagacga ttaagccatg tctggaacgg
    60121 ctggagcatg tacgcgactg tcagctatcc acttcttttc gctttatggc aagccatgaa
    60181 gggctggacg gcctgctgat cctgcctgaa atgagtcagg atcaggtgaa acgcctgtcc
    60241 accctggtag ctgcgcatat gagcatgtgc cttgatgcag cttgtggcga tttgtatgtc
    60301 accgatgacg ttaagccaga agaaatccgc aagacatggg aaaaggtggc agcggaaacc
    60361 ctacgtctgg atgtcatccc gcctgcgttt gagcaactcc gccggaaaag aaaccgccgt
    60421 aaacccgtgc cctatgaact cattccgggt tcgctggcgc gtatgttgtg cgctgactgg
    60481 tggtaccgga aattatggaa gatgcgttgc gaatggcggg aagagcagtt gcgtgctgtc
    60541 tgcctggtca gcaaaaaagc atctccctat gtcagctatg aagccgtgat gcataaacgt
    60601 gagcagcgcc gtaagtcgct ggagtttttt cgttctcatg aactggtgaa cgaagacggc
    60661 gacacgctgg acatggagga tgtggtaaac gccagcagca gcaaccctgc gcatcgccgc
    60721 aatgagatga tggcctgtgt taaaggcctg gagcttatcg cggaaatgcg cggtgactgc
    60781 gccgttttct acaccatcac ctgtccgtca cgtttccatt ccacgctaaa taatggcagg
    60841 cccaacccaa cttggacaaa tgcgacggta agacaaagca gtgattatct ggtcggcatg
    60901 tttgctgcat ttcgtaaggc gatgcacaaa gccggattgc ggtggtatgg cgtgcgggtg
    60961 gctgagccgc atcatgacgg tacagttcac tggcacctgt tgtgttttat gcgcaaaaaa
    61021 gaccgccgcg ccatcactgc attactgcgt aagtttgcca tccgtgaaga ccgcgaggaa
    61081 ctgggcaata acactgggcc gcgctttaag tctgagttga ttaacccgcg caaaggtacg
    61141 ccaacaagct acatcgcgaa atacatcagt aagaacattg acgggcgtgg tctggctggc
    61201 gagatcagca aggaaacggg gaaatccctg cgtgataatg ctgaatacgt taatgcctgg
    61261 gcgtctctgc atcgtgttca gcaattccgc ttctttggca ttccggggcg tcaggcttac
    61321 cgtgaactgc gattgctggc tggtcaggcg gcaaggcaac agggggacaa aaaagcaggt
    61381 gcgccggtac tggataaccc gcgccttgat gccatcctgg ctgctgctga tgctggttgt
    61441 tttgccacct acatcatgaa gcagggcggc gtactggttc cccgcaaata tcacctcatc
    61501 agaaccgctt atgaaatcaa cgaagagccg accgcctatg gcgatcacgg cattcgtatt
    61561 tatggcatct ggtcacctat tgcagagggc aagatctgca ctcatgccgt gaagtggaaa
    61621 atggttcgta aggccgttga tgttcaggag gcggcagccg accagggcgc ttgcgcccct
    61681 tggactcgtg gcaataactg tccccttgct gaaaatttga accaacaagg gaaagacaaa
    61741 tcagctgatg gggactccag aacggatatt acccgtatga atgacaagga gttgcacgat
    61801 tacctgcaca gtatgagcaa aaaagagcgc cgggaactgg ctgcaaggtt acgccaggtg
    61861 aaaccgaaac ggcgtaaaga ctacaaacag cgaattacag accatcagcg acagcagctc
    61921 gtctatgaac tgaagtccag gggatttgat ggcagcgaga aagaagtcga tttgctcctt
    61981 cgcggcggca gtattccgtc aggagcaggc ctgcgtatct tctatcgaaa ccagcgtttg
    62041 caggaagatg ataagtggcg gaacctgtat taattacgcg ggttaacaat tcgtgctctt
    62101 aataatacca ggcatatcag gccgatgaac gtaaaaaaac gttttacatc agtaagatta
    62161 ttatatactg taaatataaa tagtggttat atatacagta ttgttttggt gtcataggag
    62221 gaaagatgca ggactatttt ttggagtctt tgaagctcca gcgcattgat ttttttctta
    62281 agcttgtagc ggctagtgag tgtagtgatg aagagaaggg gctggctctg cagtgggttt
    62341 ctgaattgac tgatgaactc atggcaaaaa tcagaaccca cgaatacaac cgctcaatgg
    62401 atgtcatcag ctgaggtgac ttttatgcgc attgaaataa tgatcgataa agagcagaag
    62461 attagccagt ctaccctgga cgcccttgaa tccgagcttt accgcaatct gcgccccctg
    62521 tatcccaaaa cggtaattcg cattcgcaaa ggtagctcta acggtgtgga actaaccgga
    62581 ctgcaactgg acgaagaaag aaaacaagtg atgaaaatta tgcagaaggt gtgggaagac
    62641 gacagctggc tgcattaaga aacgttgctg gcgtctgaac ttgcttctgg cgtcagcaag
    62701 gttgaacaac gagcccttgc gaggcgttag ctctgtagtg catgtctatg ccgcatgaga
    62761 tcgcatgatc gtttgaggat cgtttttgct aaggcccgcc agaactggag ggcttttgcg
    62821 tagatcatgc aggtgcatga aaaccactac ataaagcggg caggcgtggc ggggatacga
    62881 gcgcgcgcaa caggtaaacc cctagacttg ttaaaatatt tgtcatataa atcaaacatc
    62941 aaacctaagg gaggcagagg atgatccagc caaaagtttt tatatcctat agttggtcaa
    63001 gtaaaactca tcagcaacat atcaaagata tagctgaacg attggcggca gatggagttg
    63061 aaactgtcat agatatttat gacttgaaag aaggggatga taaaaattat tacatggaaa
    63121 gaatggttca agatgaaacg gttacgcatg ttttagttat ttgtgataaa aaatactcag
    63181 aaaaagccga tttaagaaaa gatggtgtcg gtgttgagtc gatgataatt tcccaggaaa
    63241 tatattcatc cgtgtcgcag tccaaattta taccattaat atttgagtac aaagataatg
    63301 gagagccgta tactccaatc tttctgaagt ctagaatcta tattgatttt tcaacaccag
    63361 aaaaagaaaa tgataattgg gaacgtttaa tcaggttgct ttatggaaaa ccagaattta
    63421 ctaagccacc attaggtaag cctccggttt atttagagca agacacgtca aaaccgacgt
    63481 atgaaattca tgcaaaattt caaactttaa aatcagctgt cttaaatcaa aagcagacat
    63541 taaaggattg cagaaggcaa tttttagagg tgtgccgaaa ttattgtatc tcactacaag
    63601 tcgtaacaaa cccaactact gaagattttg cagcggaagt tttgcaaatt cataaagagc
    63661 ttattgccgt tagggatgcc attacagact gggttttact tgagggtgat acgcagggcg
    63721 aagacttttc caaagcacta ttacagttta tggaagtaat gttagcgatt agaaatcgtc
    63781 cgaaaaatgt aaattcatat aatgaaatat ggtttttgcc ccataagatt tttgcgtatg
    63841 aaacgttttt atatattttg gcggctttaa taaaaattga agctttccag catgtacata
    63901 cattactgca tacatcttat ttgctacccg atcatataac cagtccggga atggagtttg
    63961 ctaattacag tgagttatat ttaagctctg attaccttca aagtaaattg tcccctgaaa
    64021 actatcgact gtattcccct gttgctgagt tagtaaagca aagtgcaact agagatgatg
    64081 tgtctttcga tgatttgaaa caagctgatc ttgttgctct tatgatatcc ttcattaatc
    64141 ccagtatttt ttggtacccg caaatgttgt tgtattccgg gcactatgag aaatatcctc
    64201 tatttacaag agcaatacag catagaggat tcaagagtat tgcagttatt accggaatcg
    64261 atgacagtaa acttttggct caaaagctga cagagggtga ggcccagcga aatactagca
    64321 actggtatca tttcggtttt aatagagact ttctcaatca aatgaatgtt agtcgcctag
    64381 atagtattga atgaaaatta gagggcgctt tgcaaagcgc tctctgtatt agttttcaat
    64441 atctaaggtg tagggctcaa agcggatcac ttcttcgccc agccagtcgt taagctcctg
    64501 cagtcgcttc tgcagcggca tcagctcgtt gcggacaaag acgcggctgg ccttttccac
    64561 atcaccaaag ccgccggtat tgttgggaat gatgcccatc atctgcggcg gcacgcggtg
    64621 agcagccatc atgtcatcac ggctcacgtt cttgatgttc agaaattcat cctttgctgc
    64681 aacttccgac aacggaatta tctggatgcc gtccttttta ccgttgggcg aatacataaa
    64741 caggttgcgg aagttgcccg gccctttggc gcttttcatt gcctggcgga tattgttcac
    64801 gtcctcctga ttctgtgctg cgtcggtcat gtacatgatg aaacccgcgt ggctgccgtt
    64861 gatgtaatac ttccggcgaa acagcgttgc tgactcgttg agcagggttg acgggatggc
    64921 ggagagatag ccgggcagcc cgtaaatctc ctgattaata tccggttcca gcagatgaaa
    64981 gatgttgccc tgcgtaaatt catagggctg cgtggtcatg ccatactgca caaaccagta
    65041 ggtgtcgaga tccacgccgc gccgtgtgta cttcgccagt gctggctcca gcgagagaac
    65101 gccgccgacc cggttggtgc gcttttccag ataggcgtta ccgaacacca gataatcctg
    65161 gacgaaacgg gcaaaagcct gctggctgag cagcggatga gggatatagg tactggtcag
    65221 aatgttgcac tttactgcaa tcggggaact gtgatgcacg gcggcgcgga aggtgcgcgc
    65281 cagtccatta aagctgacgg gcggctcata ccagcgatcc atctgtacgc attccacata
    65341 gtccagcagt tcgcggcggt ccagcacggg gatgggatca ccaaagctga aagcctcagc
    65401 cgttgggccg ttgcttggtt ttgtgtcgtg atcaactgtt acctgcttgg tgtttttacg
    65461 tttgctcatt ctgccagttc cttgctagcg agaggccatt cgcacataaa cagcattttc
    65521 cagtcctctt ctgataattc ttttttcata tcgttcagcc agttatcagt aatcaacatt
    65581 gctccagatg ctgctgcgtc atctgcggtg aacaccatgc tggttacgct gttgtttctg
    65641 catagctttt tatattcctg ccgtgcctct gggttagggc tgggagtggt gtagtaggtg
    65701 gcgtggtagt gtgcgtgcat agaaatccct ttagcgatgg caatcatatt tctcggtgat
    65761 tcactccagg catactctga cacgtaaaca tttccactga gtgcggaggt gagactgctg
    65821 agttcggtaa aataaattac tgcaccattc ggaagctcca gatgactttt gcttgatttt
    65881 atttttccag tgtgcgtata agctgcggcc tggcttaaga atgctgatac acaggctttg
    65941 attgcctgcg cagatgctgt gctgcagcag aggaaaatct ggttacgccc ggtatgcagt
    66001 gcatcgttca gggcttcata ggaaaaaaag aaatccgcgc ctgcctggcg catttttgtg
    66061 agtacacggt ttctactctt tgggccagtg ttccactcat actggtaagc aaagaaaaag
    66121 ctatccaccg gaagtcccgc gatagtcatg aggttagttg ggattggggg catcaaaaaa
    66181 tctccacaat attgctggta ttggcggatt cgccctgcag cggttcgtta aacagtgcgt
    66241 gcatcgttgc ccaggccaga tcggcgtggc tggcttcttc gctgcggctg gcttcatagg
    66301 ttgggcggtt gccgctcgcg gtggtggcgc gacggattgc catgaatgac tgcgctatgt
    66361 cggtgtgtcc ggcgtcaaac tccagacggc ggtggctgat aatgtcgtag gccttgagta
    66421 ccagggcgtt tttaacattg gggttataga caaactcccg gacggctgga aaaaacgctt
    66481 tcacgttctc gtaaaccccg tggccgacac ctgtcgagtc gatgccgatg taggtcacgt
    66541 tgtactgctc ggtcagtttt ttgatggcgt cagcctgggc gcggaagtcc atcccacgcc
    66601 actggtgacg ctcaagaatg cggaacttac caccaggcac ggctggcggt gccaccacta
    66661 cgcatccggc gctgtcgccg ttttgcgtac cttttgccgg gtcataaccg atccacactt
    66721 cgcgccagcc aaacgggcgc agagccagtg catgaaagtc ggtccagact tcccagctgt
    66781 ccaccatgca cgcctgcagc tcgctgagcg ggaacacgga cgcgagatcg tccacaaact
    66841 cgcacatcag caggttctgg tattcgtccg ggctgtactc catgcgcaac tggtcaaggt
    66901 cgaacaggtt acagccgccg cgcaccgcat cttccacggt gactatctgg cggtattgcc
    66961 cgtctgcgca cagcaggccg ggggccagat tgctgtggga caggtcgatg tccaccttgt
    67021 cggccttgtt gcgcccacgg ttgaacagcg caccggacca gaacggataa gcactatgtg
    67081 tcaggctgga tggcgtggaa aaataggttt gtcgccattt tttgtgaata gccataccgg
    67141 aagccacttt gcgcagctcc tggaatttcg gtatccagaa atattcatcc agatacaggt
    67201 tgccgtgata actctgggcc gtgcgggcat tggtgccgag gaagtaaagc gtggctccgt
    67261 taggaagcac catcggatcg cctttcagct ccacctcgac ttctttggcg aagtctatga
    67321 tgtactgctt aaagacgtga gcctgtgcct tactggcaga aaggaaaatc tggttacgtc
    67381 cggtaagcag ggcgtcaatc agggcttcac gggcaaagta aaaggtcgcg ccgatctggc
    67441 gtgacttcag caggttgcgg atgcggttgg tttttccggc ttcccaccag tggcgctggt
    67501 agttgaacat ggaggaatgg aaaatttctt ccagcttctc aatctgttca tcggtgaaaa
    67561 cattcttttc cggctgacgg tgcgggcctt tgttgcggtt ggcgacgtta gggtttaagt
    67621 cggcttcgtt accgccattg ttaaacttgc cgatccgcgc gtggcgctcc gactggcgcg
    67681 ccagcaggtc aatttctttg aaatctttcc cttctttgtg ctccttcata atgagctggc
    67741 agtagcgtgc ggcggtggtg agctgcatct gatccagcgg cccatagtca ccccacttgt
    67801 cgcgtttttt ccagctgtga acggttgcaa ctttctcgcc cagcatttca gcaatgcggg
    67861 ctacgcggta tccctgaaag tacagcagca tggcctgccg acggggatcg agatctgcgg
    67921 gtgtcagtgt ggtgttcatg gcacaaacct acagccttga atgaaggctt tccccgcctg
    67981 cggtttgtgt ggttgtcggt acaaataccg cgcattgttt cactgccccc atcaccgcaa
    68041 ccataaggct ccagtaagtt ttttctaacg gagcacggct catgacagtg aaagcaaagc
    68101 gttttcgcat cggggtggaa ggtgccacca ccgacggacg cgaaatccag cgagaatggc
    68161 tggaacagat ggcagccagc tacaacccgg cggtgtatac cgcgctgatt aaccttgagc
    68221 acatcaagtc ttatctgccg gacagcacct ttaaccgcta cggcaaggtg acggcgctgt
    68281 ttgctgaaga aatcacggaa ggtccactgg caggcaagat ggcgctgtat gccgacgttg
    68341 agccaacgga gtccctggtg gagctggtga aaaaaggcca gaaattattc acctctatgg
    68401 aagtcagccc gaagttcgct gatacgggca aagcctacct ggttggcctg gctgccactg
    68461 atgaccctgc cagtctgggc actgaaatgc tgacattcag cgccagtgca gcccataacc
    68521 cgctggcaaa ccgcaagcag aaccccgcca atctctttac cgctgcagag gaaacggtga
    68581 tcgaactgga agaagtccag gacgacaaac cgtccctgtt tgcccgtgtc acggcgctgt
    68641 ttaccaaaaa agagcagtcc gatgatgccc ggttctctga tgtgcataag gccgtggaac
    68701 tggtcgccac tgaacagcaa aacctgagtg cgcgcaccga aaaatccctg tctgaacagg
    68761 aagaacgcct gtctgagctg gagactgccc tgcaggcaca gcagaccgct tttaacgaac
    68821 tggtggacaa gctgagccat gaagacagcc gccaggacta ccgccagcgt gcaacaggcg
    68881 gtaacgccac cgctgacact ctgaccaatt gctgatggag cacaaaacct gatgaagaag
    68941 aatacccgct ttgcttttaa tgcttacctg cagcagctgg cgcgtctgaa cggtgtggca
    69001 gttgaagagc tgtccagcaa gttcactgtg gagccgtctg tgcagcagac gctggaagac
    69061 cagatccagc agtccgcctc tttcctgacg ctgattaacg tcacgccagt gactgagcag
    69121 tccggtcagc tgctggggct gggtgttggc agcaccattg ccggaaccac tgataccacc
    69181 gcgaaagagc gtgaacctgt cgatccgacg ctgatggtcg atgtggaata caaatgtgaa
    69241 cagaccaact ttgacacggt gctgacctac gcgaagctgg acctgtgggc gaagtttcag
    69301 gatttccagg tgcgtatccg tgacgccatc gtgaaacgtc aggcactgga ccgcatcatg
    69361 atcggcttta acggcgtgaa gcgtgcgaaa acctccaacc gtagtgaaaa cccgctgctg
    69421 caggatgtga acaaaggctg gctgcagaaa atccgtgagg atgcaccgga tcacgtcatg
    69481 ggcagcacca ccacgggtgg tgaaaccaca ccgggcgcgg tgaaagtcgg caaaggtgga
    69541 gaatatgcca acctagacgc cgtggtgatg gatgctgtca atgagcttat cgacgtggtc
    69601 taccaggacg atgacgatct ggtggtgatt tgcggtcgtg agctgctgtc tgacaagtat
    69661 ttcccgctgg tcaacaaaga gcaggaaaac agtgaaaaac tggctgccga tatgatcatc
    69721 agtcagaaac gcatgggtgg cctgcaggcc gtgcgtgcgc cgttcttccc gccgaatgcg
    69781 ctgctgatca cccgtctgga taacctgtcc atttactggc aggaggacac ccgccgccgt
    69841 tcggttatcg acaacccgaa acgtgaccgg attgaaaact ttgaatccgt taacgaagcc
    69901 tatgtggttg aggactaccg ctgcgccgca ctggtggaaa acatccagat tggcgacttc
    69961 agcgccgccg ccgcagaagc cggagcataa accatgagcc tgagtcccgc acggcagcat
    70021 cgcctgcgcg ttcaggctga acaggccgcc cgcgagggcg gcagtgttcg ccacgcgtcg
    70081 ggctatgacc tgatgctgct gcaactggcg gaagaccgcc gccgtctcaa gggcgttcag
    70141 tccacggtca aaaaagcgga aatcaaggtg gagctgctgc cgaagtacgc cgcctgggca
    70201 gagggtgtcc tggctgccgg aggcgctcaa caggatgacg tgctgatgta cgtgatgctg
    70261 tggcgcattg atgccggaga ttatgccggg gcgctggaga tcgggcgtca tgccctgcgt
    70321 catggctggg tgatgccgct gggtaaccgc aacgtgcaga ccgtgctggc agaggaaatg
    70381 gcagatgcag cccagagcgc aatgcttgcc gccaccggct ttgatgccga tctgttgctg
    70441 cagacgctgg agctgacaga cggtatggat atgccggacc agtcacgggc gcgtctgcat
    70501 aaagcgattg gcgctgtcct gagtgaaaac aatccggcgt ccgcccttaa tcatctcaac
    70561 catgcgttac agctcgatcc ccgctgtggc gtgaaaaaag acaaacagca gctggagcgc
    70621 agactgcgca atgacagccg ctgacagaac gtgcccccgc gcacgggcgg cacggggtgg
    70681 cgaaaggcac tgccacatca aaaccccgtc caccgccctc tatttcagga gaaagcagca
    70741 tgaagtttgt tgcgccagaa caggcaccgg aacaggcgga aatcatcagg aatacgccgt
    70801 tctggcctga tgtggacctg tcggagtttc gcagtgtcat gcgcactgac ggcacagtga
    70861 cacagccgcg tttaaagcag gttgcgctgt cggcaatttc ggaggtcaac gcagagctgt
    70921 atgagtttcg cagacgtcag cagatgctgg ggtatgcgtc gctggctgag gttccggcgg
    70981 aacagctgga cggcaaaagt gagcgcatcc agcactattt caacgcggtt tactgctggg
    71041 cacgcgccat gctcaacgaa cgataccagg actatgacgc cacggcatcc ggtgtgaagc
    71101 gaggcgagga actggcggaa tccagcggtg atttgtggcg tgacgcccgc tgggccatca
    71161 gccgggtgca ggatgcgccg cactgcacag tggagcttat ctgatgaaag tgcgtgcgca
    71221 tcagtatgac acggtggacg cgctttgctg gcgtcattac gggcgcacgc agggtgtcac
    71281 ggagcaggta ctgaaggcaa atccggggct tgccgaatat ggcccctttt tacctcacgg
    71341 gctgcaggtg gagctgccgg acattccgac caccaccacc gtgcagaccg tccagctatg
    71401 ggactgaatt atgacgcttg agcgaatcag cgcctttatc acgtactgca tcgccgttgt
    71461 gctggcctgg ctgggcgatt tgtccatcaa ggatgcctca acgctgggcg gcctgatgat
    71521 tggtgtgctg atgctggcta tcaactggta ctacaaacac aaagcctacc agcttctgcg
    71581 cgacgggcag atctcgcggg aggactatga atccatcaat cgttaaacgc tgccttatcg
    71641 gggccgtgct ggctattgct gccacgctgc cgggttttca gcagcttcac acctccgtgg
    71701 aggggctgaa actgattgca gattacgaag gctgtcgtct gcagccgtat cagtgcagcg
    71761 cgggcgtctg gaccgacggc attggtaaca cgtcaggcgt cattcccggc aaaaccatta
    71821 cggaacgaca ggcagctgaa gggctgatct ccaacgtgct acgagtggag cgggcgatgg
    71881 aaaggtgtgt gaagcaacag ccgccgcaga aggtgtatga cgctacggtg tcgtttgcct
    71941 tcaacgtggg gacgggcaat gcctgtagtt ccacgctggt gaaattgctc aatcagcggc
    72001 gctgggcgga tgcatgccga cagttgccgc gctgggttta tgtgaaaggt gtttttaatc
    72061 aggggctgga taaccgccgt gcgcgggaga tggcctggtg tttacaggga gcaaactgaa
    72121 atgaaaaaga aattaatcag cggactgttt ctgatgttat ggatggcgct gttaatcgca
    72181 gcaatggtgt atccgcaggg gatttttccg gtactggcag cgtccggcgt ttgggtagcc
    72241 tgtttgctga catgggcggt aattccggta gcactggctg cgttaattaa gaatggcccg
    72301 ctctggcagg agttaagggc atctttgctg aagacaatta cccgaaaaga aaacgtattt
    72361 accagttggg tgatgcgatt gctgattgtt gtaagtctcg cctggacggg gtgggctatt
    72421 accctggtct tttatctact gaccgttatt gccttctgga tcacccgtaa tcagatggcg
    72481 caacaggtag cagcatgaac cggttgctgc tggttgtgct gacgttatta ctggcggcgc
    72541 tgggctggca gacgtggcgg ctggctgatg ccagccagac catcagcacg caggcagacg
    72601 agctgcagag caaaagccag gcactggcaa agagcaatag ccagcttatc agcctgtcca
    72661 ttctgactga aaccaataac cgggagcagg cgcggctcta tgccgaagca gaacagacca
    72721 gcacgctgct gagacaacga caacaccgga tcgaggaact gaaacgtgag aacgaggatt
    72781 tacgccgctg ggctgatact cctttgcctg ctgacattat ccggctgcgg aaacgtccgg
    72841 cactcaccgg aggtatggct taccgtcagt ggttgtccgc gagtgacgcc gtgtcggctg
    72901 gatcaggcaa cgccgcgcac taacggtgat ctgaacgcgt tgctggatga aacggaggcc
    72961 gcctgggcgg tctgtgcaga caaagtggac atgattattg cgtgtcagga gcgaaacagt
    73021 gaacaaacca caatccctgc gccacgccct caataaagcg gtgccttatg tccgcaataa
    73081 cccggacaaa ctgcatctgt ttgtggataa cggttcgctg gttaccacgg gggccagctc
    73141 catgtcgtgg gagtaccgtt acacactgaa cgcggtgatt gaggatttca gcggcgacca
    73201 gaatctgctg atggccccgg ttttgctgtg gctgagggat aaccagcccg atgccatcaa
    73261 taacccggcg ttacgggaaa agctattcac ctttgaggtg gatattctgc gcaacgatgt
    73321 ctgtgatatc agcctgaacc tgcaactgac ggaacgtgtg ctggtcagca ctgacggcag
    73381 tgtgtcgagc gttgaagcta tagcggaacc tgatgcacct gaagaaatgt ggacggtgaa
    73441 acgtggctga actgcagaag gtggacgact ggctgagtgc cttgctggcg aatctggaac
    73501 cagcctcgag aagccgcatg atgcgccagc tggcgcagga acttcgccgg acacagcagc
    73561 agaatatcag gatgcagcgc aaccctgacg gcagcggcta tgaaccgcga cgggtaacag
    73621 cacgcagtaa aaaaggccgt atcaaacgtc agatgtttgc aaagctgcgc accacaaaat
    73681 acctgaaaac tgccgccagc accgactctg ccagcgtgca gtttgaaggc aaggtgcagc
    73741 gcattgcccg cgttcaccat tacggcctgc gagatcgcgt cagtcgtaag ggaccggagg
    73801 tgcgttacgc agagcgccgc cttctgggtg taaatgatga tgttgaggca atgacccgcg
    73861 acatgattct gcaatggctg gcggggtgat ctttgtatca gcactgatac aagttgcagc
    73921 actgccgcct ttcttcccct gatggcaacc tttccctatg aacgcacaat taaccgaaat
    73981 catgcgcctt atcaccaacc tgatccgcac aggggtagtc accgaagtgg acagggaaaa
    74041 ctggctttgc cgggtgaaaa cgggcgacct tgaaaccaac tggattaact ggctgacgct
    74101 gcgcgcgggt aatgcccgga catggtggcg accatcggaa ggtgagcagg tggtgctact
    74161 gagtctgggc ggcaatctgg aaacagcctt tgcgctgccc gccatctatt cgaatcagtt
    74221 cgcgccaccg tcgacgtcgg cagacgcctg cgtgacagaa catcctgacg gtggctggtt
    74281 tgaatacgaa cctgccaccg ggcgttggta tgtaaggggc atcaaatcaa tggtcattga
    74341 ggccgctgac aacatcacca tgaaaaccag tgagtttgta ctggaggctg accacacgcg
    74401 cattaacagc gaagtggtga tcaatggtgg cgttacccag ggcggcggag tgatgagttc
    74461 taacgggatt gtggttgatg cacatcagca tactggcgtc ctgaaaggcg gcgacacaac
    74521 cggaggcccg gtatgacgct ttatagcggg atgaacaata ccagcggtaa agccattact
    74581 gatattgacc atctgcgcca gtcggtgcgg gacattctgc tgacaccgca gggtagccgt
    74641 attgcccgtc gtgaatatgg ttccctgctg tcggcactga tagaccagcc acaaaatcca
    74701 gcattacgcc tgcaggtcat gtcggcagtg tatgtggcgc tgagtcgctg ggagccacgg
    74761 ctgacgctgg attccatcac cattaacagc aattttgacg gttcaatggt ggtggggctg
    74821 accgggcggc gtaataacgg tgtgcctgtt tccctttccg tatcaacagg agcagagaat
    74881 ggcagtgatt gacctttcgc agttgcctgc gccgcagatt gtggatgtgc cggactttga
    74941 gacgctgctt gccgaacgca aggccgaatt tgttgcgctt catccgaaag atgagcagga
    75001 agcagtgatc cgcacgctgg aactggaatc tgaacccgtc accaaattgc tgcaggagaa
    75061 cgcttaccgt gagttgcttc tgcgccagcg cattaacgaa gccgcgcagg cggtgatggt
    75121 ggcttacgcg atgggcggcg atcttgacca gctcgctgcc aactacaacg tgacacgcct
    75181 gacggtgacg cctgctgata atgatgctgt gccgcccgtt gcagctgtga tggaaagcga
    75241 tgaagcgttg cgcctgcgtg tgcctgcagc ctttgaaggg ctttctgttg cggggccaac
    75301 tgcagcttat gaatttcatg cccgaagcgc cgacggtcgg gtggcggatg ccagtgcaac
    75361 cagcccggca cctgcagagg tggtgctgac tgtccttagc cgcgaaggcg atggaactgc
    75421 agaaaaagac ctgctggacg tggtggaaaa agctctgaac agtgagaacg tccgcccggt
    75481 ggctgaccgt cttacggttc gcagcgcaga aatcatcccg tatcgcgtgg aagccaccat
    75541 ttttctctat ccgggaccgg aagcagagcc ggtaatggca ggggcaaaag ccagcctgca
    75601 gaagtacatc gccagtcaga cgcgtcttgg tcgggatatt cgccgtagcg ccatctttgc
    75661 cgccctgcat gttgagggtg ttcagcgtgt ggaactggct tccccgcagg cggacgtggt
    75721 cctgaacaaa acgcaggcgg catcatgtac gcagtggagc gtgaccaacg ggggaacgga
    75781 tgaatagtct gctgccaccg ggttcaactt cactggagcg ccgactggcg caaacctgta
    75841 gcgggatttc tgatctgcag gtgccgctgc gtgacttgtg gaatccggca acctgtccgg
    75901 tcagtttcct gccttatctc gcctgggcgt tctctgtgga tcgctgggac gagggctgga
    75961 cagaaagcgt caaacgccag gtagtgaagg atgcttttta tattcatcag cacaaaggaa
    76021 ccaccagtgc cgtacggcgg gtggtggagc cgttcggctt cctgatccgc attattgagt
    76081 ggtggcagac cggagaaaca ccgggcacgt ttcgcctgga cattggtgtg caggaccaag
    76141 gcatcactga agatacctat ctggaacttg agcgactgat aagcgatgcc aaaccatgta
    76201 gccgtcacat gattggcatg tccatcaatc tgcagaccag cggcccgcat tgggtgggag
    76261 ccgccagtta tcttggcgaa gaaatcacga tctatccgta tatcaacgaa acaattattt
    76321 ccggcggcac cgcgcatgaa ggcggggcgg tccatgttat tgacacaatg agagtgaatc
    76381 catgagcaca aaattttata ccctgctgac ggatattggc gcggcgaaac ttgccagcgc
    76441 cgccgcgctc ggtgtgccgc taaaaattac ccatatggcg gtgggcgatg gcggcggagc
    76501 attgccaacg ccggacgcaa agcagacggc actggtaaat gagaaacgcc gggctgcgct
    76561 gaatatgctt tatatcgacc cgcagaacag cagccagatt attgccgaac aggtgatccc
    76621 tgaaaacgag ggcggttggt ggatacgtga agtgggcttg tttgatgagt ccggggcatt
    76681 gattgccgtg ggcaactgcc cggaaagcta taagccgcaa ctggctgaag gtagcgggcg
    76741 cactcagacc gtgcgcatgg tgctgattac cagcagcacg gacaatatca ccctgaaaat
    76801 cgaccctgct gtagtgctgg caacccgcaa gtatgtggat gacaaggcac tggagctgaa
    76861 ggtgtacgcg gatgatcaga tggcaaaaca tcttgccgca ccggacccgc attcacagta
    76921 cgcgccaaaa gccagcccga catttaccgg aacccccaaa gcgccaacgc cagcggcggg
    76981 gaataatacc acgcaggttg cgaccactgc gtttgtacag gcggcactga cggcccttat
    77041 taatggtgcg ccagccacgc tggacacgct gaaagaaata gccgcagcca ttaacaatga
    77101 tccgaatttc agtaccacca ttaacaatgc gctggcacta aaagcaccgt tgtcgagtcc
    77161 ggcactcacc ggaacgccaa cagcccccac ggcggcgcag tcggtcaaca atacacagat
    77221 tgccactacg gcttttgtga aatcggcgat tgcaggaatg gtgggttctg cacctgctgc
    77281 actggataca ctgaacgaac tggcggcggc actggggaat gatccgaact ttgccacgac
    77341 aatgcttaat gcgctggcag gtaaacaacc gctggacaat acgcttacca atttgagtgg
    77401 aaaggatgta gctggtcttc tcacatacct tcgtttgggt gctggcgctc ctgctattgg
    77461 tattccgttc ttctggccgt cctctgcgat gcctaatact gtcatgccag aatgggccga
    77521 tatggtgttt ctaaagttca atggcgcgac attttccgca acaacatatc caaaacttgc
    77581 cctcgtattc cccgggctaa ccctaactga agctcgcggc gaatttttgc gcgtatggga
    77641 tgatggacgt gggattgata gcgggcgtca gcttttgagt gcgcaagggg acgcaataag
    77701 gaatttgacc gggcaatttt ttgcgggaac atcaggtaac tcacaatatg ttttatcagg
    77761 tgcgaacggt gtgttttctc aggggggaat gacgtttggt cctaacctcg ccggtgcatc
    77821 aggaacggga gaccagagat cagctggggt agattttaat gcttcccggc aggttcctac
    77881 tgcgtcagag aaccgacctc gtaatattgc attcaacttt ttagtaaggg caaaataatg
    77941 aaaccagttt ttgatgaaaa tggactggca acagaaccgg gggaaattcg ttgctattat
    78001 tacgatgccg tgtcctatga ataccaggga tggtctgatg aatatattaa tactggcgta
    78061 agtatgcccg cctgttccac tggtattgac ccgagcgaaa acattccggg aaaagtggca
    78121 gtatttacgg gtaagggatg gagccatgaa gaagaccatc gcaatgagac cgtttactca
    78181 actgaaaatg gcgcagctgt tacagtggat tatatcggtg ccatcaaaga cggttatgtc
    78241 acgctttcac cgttaacgcc atacgataaa tgggatggtg agaaatgggt gacggatatc
    78301 gaggcacagc atagcgccgc agtagaagcg gcagaagcac agcgccagtc gctgattgat
    78361 gctgcaatgg cttccatcag tctgattcaa ctgaaattac aggccgggcg gaagctgacg
    78421 cagccagaaa acacccgact taacgctgtg ctggattata ttgacgcggt gacggcaaca
    78481 gataccagca cagtgccgga cgtcatctgg cctgaactgc cggaggcgta ggccattcaa
    78541 tatctggcgc accggaagta tcgaccagtt ccagtgcgtc cagataatcc agccacaaat
    78601 tatattgcgc cagttcctca cctttcaggc gaccaatcgc cgctttacca ggccattgtc
    78661 tgctgttcat gtgctcgtgg gcttcattaa taagttttct ttttttcaaa tccgccaatg
    78721 caataaggtt ttcttttgat aaaggtggtt gctctgtcaa aaccggatat ccctcctgat
    78781 tgctgactat tttcatgcca ttatcctgac catccagtag taacagccat tcatccgtgg
    78841 ttatctcaac aacatctgaa ggtgctttat ttaaatcggt aaaaaaaaca ttttcttttt
    78901 gtgaatagaa gtatctgtcc atttattaat ctccaaaagc aatccagtaa gcaaaaggat
    78961 ttattccttg ctcagtcaca gatgacatca gggaaaattg cgatggtgaa acaggtaacg
    79021 ctgcaaaact aaccattgtt gacacacctg accgtgcatt atcgtacgat gcaacaacac
    79081 aataattggt attgctgaaa gatatcggca gggtgatatt tacaggtgag cctaatggcc
    79141 ctgatgctga tattcccatt tgaatgatgg ttccatcagg caattttctc cagcgattag
    79201 aactcggatt tcttttccag gctgacatat ccggtatctg attttctcct gtgcccacat
    79261 cccttttcgc cgcttctccc aaaccaacgt ttaagaaaat gcagaggtaa cagctaactg
    79321 gcatcatctc cggtttttat tcagggggat gatcatgctt attggctatg tacgcgtgtc
    79381 aacaaatgac cagaacaccg atttgcaacg taatgcactg aactgcgcgg gatgtgagcg
    79441 gatttttgag gacaaaatca gtggcactaa gtccgacaga ccggggctga aaaaactact
    79501 caggacacta tcggcaggag acacgctggt tgtctggaag ctggacaggt tggggcgcag
    79561 tatgcggcat cttgttacgc tgatagaaga gttgcgccag cgtggtgtga atttcagaag
    79621 cctgattgac agtattgata ccagtacccc aatgggccgt ttcttttttc atgtcatggg
    79681 tgccctggct gaaatggaac gcgaactgat agttgaacgt accagggcag ggctggctgc
    79741 agctcgtgcc aaaggcagag taggtggacg ccgtcctaag ttgaccaccg aacagtgggc
    79801 acagattggg cgtttactag aggccggaga atcaagacag cgtattgcac tgatttttga
    79861 tgtaggcgtt tctaccattt atagaaaatt tccggcaaat aagatcaatg aatccccctg
    79921 aatcagcatt atgttgatta ttcctgcaag cagacaaata ccgttatttt gtgtgaataa
    79981 cgacacaact gcgtttagct gtttgtcagg cacaatcact tcaacatagg gcgaagccta
    80041 atccaatcag gaggttcgcc actatggctc aggattacca ccacggggtg cgcgttgttg
    80101 aagtcaacga aggcacccga tctattacca cggtgagcac cgccatcgtg ggtatggtct
    80161 gcacgggcga tgatgccgat gcaaaaatgt ttcctcttaa taaacccgtg ctgatcactg
    80221 atgtgctgac tgccagcggt aaagcgggtg agtccggtac tctggcccgt tcgctggatg
    80281 ccatcgctga ccaggcaaaa cccgtgacca ttgttgtgcg tgtgccgcag ggtgaaacgg
    80341 aagacgaaac cacgaccaat atcatcggcg cagtgactgc tgaaggtaaa aaaacaggta
    80401 tgaaagccct gttatctgcc cagtcacagc tcggcgttaa accgcgcatt ctcggcgtgc
    80461 caggccacga caccaaggcg gtggctactg agttgctgag cgtggcgcaa agcctgcgtg
    80521 gatttgctta cctgtcagcg tatggctgca agacagtgca ggaggcgatc acttaccgcg
    80581 aaaacttcag ccagcgcgaa gggatgctga tctggcctga ctttactggc tgggacacgg
    80641 tgctgaatgc cgaagcaacg gcatatgcca ccgctcgtgc gcttggtctg cgcgccaaaa
    80701 ttgacgagca gaccggatgg cacaaaagcc tgtccaacgt gggcgtgaac ggtgtcaccg
    80761 gaatttctgc agatgtgttc tgggatctgc aggacccggc aaccgatgca ggtctgctga
    80821 accagaacga cgtcaccacg cttgtgcgta aagacggttt ccgcttctgg ggttcccgct
    80881 gcctgagtga tgaccagctc tttgccttcg aaaactacac ccgcacggcg caggtgctga
    80941 tggacacgat ggcagaagca cacatgtggg cggtggataa accgcttaac ccgtcgctgg
    81001 cccgcgacat tatcgaaggt atccgcgcca aaatgcgcag cctggtcagt cagggctatc
    81061 tcattggtgg tgattgctgg ctggatgagt cggtgaacga caaagacacg ctgaaagccg
    81121 gaaaactcac catcgactac gactacacgc cagtgccgcc acttgaaaac ctgatgctgc
    81181 gtcagcgcat caccgatcag tacctggtga atttcgccag ccaggtcagc gcgtaagggg
    81241 acaacatggc tttaccacgc aaattaaaac acctgaacct gtttaacgac gggaacaact
    81301 ggcaggggat cgttgagtcg ctgacgctgc cgaaatttac ccgcaaatat gagaagtatc
    81361 gcggcggcgg aatgccgggt gcggtggatg tggatctggg gcttgatgac agtgcgctgg
    81421 acacagaatt ttccattggt ggtactgaat tgctgctgtt taaacagatg ggtaaagcca
    81481 cggtggatgg catccagctg cgctttaccg gctctattca gcgtgacgat accggggaag
    81541 tgcaggccgt ggagcttgtc gtgcgtggac gtcacaaaga agtggattcc ggcgagtgga
    81601 agacgggcga aagcaacacc accaaagtga ccagtaccaa cagctacgcg aagctgacca
    81661 tcaatggtga ggtgctctat gaagtggacc ttatcaacat ggtggaaatt gtggacggtg
    81721 tggacctgat ggaagcgcac cgcaacgccc tcggcctctg atatatctga acggcgcggg
    81781 ataccgcgcc agaacccaat tgacaggaca gcaaaatgag cgataagcag actgaaaaga
    81841 ccattcaact ggataccccc atcaagcgcg gtaaaacaga aatcaccgaa attgtgctgc
    81901 gtaaaccgca gtccggtgcg ctgcgcggta cacgcctgca ggccattatg gatatggatg
    81961 taaacgcgat gatgaccgtg atcccccgca tctccagtcc ggcactgact gcacaggaaa
    82021 ttgcagagat ggacccggca gatctcactg ccatgtcggt tgaggttgtc acttttttgt
    82081 tgaagaagtc ggtgcttgcc ggtttaccga cagcctgacg gttgacgatc tggtggcaga
    82141 tatcgccacc atttttcact ggccgccatc cgttactgac gttatgccgc tgaccgaagt
    82201 gctggaatgg cggtataaag cgattcagag aagcggggcc aacgatgagt gataacaacc
    82261 tgcgtctgca ggtcattctt aatgcggttg acaagctcac ccgcccattt cgatctgcgc
    82321 aggccagttc aagagaactg gctgctgctg tcaaaaaatc ccgcgatgca ataaagcagc
    82381 ttgatcaggc cgggagcagt ctggacagct tccgaaagct gcaggcagaa aatcagaaat
    82441 taggcgacag gctgaactat gcccgccagc gtgcaaattt gctcagtcag gaactgggag
    82501 cgatggggcc gccttcgcaa cgtcaggttg ttgctctggg ccgtcaacgg ctggctgttc
    82561 agcgcctgga agaacgccag aaaaagctgc agcagcagac ggcgcttgtg cgtgctgaac
    82621 tgtaccgggc gggaatttct gcgaaagacg atgcgggagc aactgcccgt ttagcccgtg
    82681 aaacatcacg ttataaccag gaactttcga aacaggaggc gcggctgaag cgactggggg
    82741 aagctcagcg caggatgaat gcagcgcgtg ccagttatgc ccgttcgctg gaggtgcgtg
    82801 atcgtattgc aggtgccgga gccaccacca cggctgcagg gctggcaatg ggcgcaccag
    82861 tgatggcggc agtaaaaagc tataccagca tggaagatgc catgaaaggt gtggcaaagc
    82921 aggtcaatgg tctgcgtgac gataatggca accgcactgc acgtttttat gaaatgcagg
    82981 atgccatcaa ggctgccagc gaacagttgc cgatggaaaa cggtgcggtg gatttcgctg
    83041 cactggttga aggtggtgcg cgcatgaacg tcgcaaaccc tgacgacagt tgggaagatc
    83101 agaaacgtga cctgctggcc ttcgccagta cggcagcaaa ggcggcaaca gcctttgagc
    83161 tgccagcgga tgaactgtca gaaagtctgg ggaaaatcgc ccagctctac aaaatcccca
    83221 cccgcaatat tgaacagctc ggcgatgcgc tgaactatct ggatgataac gccatgtcga
    83281 aaggggcaga catcattgat gtgatgcaac gtctgggcgg tgtggctgac cgtctggatt
    83341 atcgtaaagc ggcggcgctg ggttccacct tcctgacact gggcgctgcg ccagaggttg
    83401 cagccagtgc agcaaacgcg atggtgcgtg aattgtccat tgccaccatg caaagcaaga
    83461 gtttctttga agggatgaat ctgctgaaac tcaatcctga agtgattgaa aagcagatga
    83521 cgaaggatgc gatgggaacc atccagcgcg tgctggagaa ggtaaacgcg ctgccgcagg
    83581 acaagcgcct gtctgccatg actatgttgt ttggtaaaga gtttggcgat gacgcggcga
    83641 aactggcaaa caacctgccg gaactgcagc gtcagttaaa actgacagcg ggcaatgatg
    83701 cgctcggctc catgcagaaa gaatccgaca ttaacaagga ttcactttct gcgcagtggt
    83761 tgctggtcaa aaccggagcg cagaacacct tcagcagtct gggcgaaacg ctgcgccagc
    83821 cgctgatgga tattctgtac acggtgaaaa gcgtcacggg ggcgttgcgt cgctgggtgg
    83881 aagctaaccc ggaactgaca ggcacactga tgaaagcatc ggctgttgtg gctgcggtta
    83941 ccgtcggcct aggcacctta gcggtggcga tggctgcagt gctggggccg ctggcagtga
    84001 tccgtctggg attctctgtg ctgggtatca aaacgttacc ttccgttacg gcagcagtaa
    84061 ctcgaaccag caacgcgttg tcctggttgg ctggcgctcc actggcactg ctgcgacgcg
    84121 ggcttgcttc atcgggcaac gccgcaggtt tacttactgc gccgttgtcg tctttgcgcc
    84181 gcacggcatc actgacggga aatgtcctga aaactgtagc aggtgcgcca gttgcacttt
    84241 tgcggtctgg attatccggt ttacgtgctg ttgctgtgat gtttatgaat cctctggcgg
    84301 tactgcgcgg tggactggcc gccgcaggca cggtgctgcg agtactggca tctggtccac
    84361 tggcgatgct gcgcgttgcc ctgtatgcca tatctggtct gttaggtgct ctgctcagtc
    84421 cgataggtct tgtggttact gcactggcgg gcgtggcgct ggttgtctgg aaatactggc
    84481 aacccatcac cgcatttctc ggtggcgtgg tggaaggatt caaagcggcg gcaggtccca
    84541 tcagtgcagc gttcgaaccg cttaagcccg tgttccagtg gattggcgac aaagtgcagg
    84601 cgctgtgggg ctggtttact gatctgctga cgcccgttaa gtcgacctct gccgaactgc
    84661 agagcgcagc ggcaatgggg cggcgattcg gggaggcact ggcggaaggg ctgaatatgg
    84721 tcatgcatcc gctggactcc ctgaaatccg gcgtttcctg gttgctggag aagctcggca
    84781 ttgtcagtaa agaggccgca aaggcaaaac tgccggaaag cgtgacgcgt cagcaacctg
    84841 cgacggtgaa tgcagacggt aaagtgatga tgccatcggg tggttttcca tcatggggat
    84901 atggctttgc ggggatgtat gacagcggcg ggtatatccc gcgcgggcag tttggcatcg
    84961 tcggtgaaaa cggaccggaa attgttaacg gcccggcaaa tgtgaccagc cggagaaata
    85021 cagctgcact ggctgccgtt gttgccggaa tgatgggcgt tgctgccgcg ccagcagagc
    85081 ttccaccgtt gcaccccttg gcacttcccg cgaaaggtgg agaagcaatt gtgagtcgcg
    85141 cagccactgt gccgctcgtt caacggattg aggcaccgac gcagatcatc attcagacgc
    85201 agccaggaca aagtgcgcag gatattgcgc gggaggtggc acgccagctt gatgaacgtg
    85261 aacgcaggct gaaggcaaaa gccaggagta actacagcga tcagggggga tacgacgcat
    85321 gatgatggtg ctgggattgt acgtgtttat gctgcgcacc gttccgtatc aggaactgca
    85381 gtatcaacgc agctggcgac atgcggcaaa cagtcgggta aaccgacgtc cgtccacgca
    85441 gtttctggga ccggaaaacg acatgctgac gctttccggt gttcttatgc cggaaataac
    85501 aggcggcagg ctgtcgttgc tggcactgga gcagatggca gaacagggga aagcatggcc
    85561 cctgattgaa ggcagcggca cgatttacgg catgtatgtg attgagggac tgaatcagac
    85621 taaaacggag tttttccgcg acggtatgcc gcgccggatt gagttcaccc tgtcgctcaa
    85681 acgagtggat gaatccctgt ccgatatgtt cggtgatctc agtgcgcaac tgaataatct
    85741 gcaggacacg gcaacgtctg ccttaagcga tatcagtaaa acggtgggag ggctgctgtt
    85801 gtgaatttca gctctgaact gcttaacaaa ggcaacaaaa ctcccgcatt cagcatcagt
    85861 attgagggca gggatatcac cactgtgctg gataaccgcc tgatgagttt gacgctgacg
    85921 gataaccggg gctttgaagc ggaccagctt gatctggagc tggatgacgc tgacggaaaa
    85981 atcgtgctgc cgcgccgtgg tgcggttatt acgctggcgc tgggctggaa gggacagccg
    86041 cttttcccga aaggggcatt cacagtggac gagattgaac acactggcgc accggaccgc
    86101 ctgactatcc gggcgcgaag tgctgatttt agggaaacgc tgaatacccg ccgtgaaaaa
    86161 tcgtggcaca agaccactgt cggggaagtg gtgaaggaaa tagccgcgcg tcataagctg
    86221 aagatggcac tgggtaaaga cctgtcggat aagcccgtgg agcatataga ccagactaat
    86281 gagagtgacg gcagttttct gatgcggctg gcgcgccagt acggtgccat cgcgtcggtg
    86341 aaaaatggca atctgttatt catccggcag ggacagggca aaagcgccag cggtaaacca
    86401 ctgccggtga tctcaatcac acgtaaggac ggcgacagtc accgctttac cctggcagat
    86461 cgcggagcct acacgggcgt aattgccagc tggttgcata cccgcgaacc cgcgaagaaa
    86521 gaaagcacca cggtgaagcg taagcgcagg actaagaagc agaagaaaga gccggaagcg
    86581 aagcagggcg attacctggt gggtacagat gaaaacgtgc tggtacttaa tcgcacctat
    86641 gccaaccgga gcaacgccga acgggcggcg aaaatgcagt gggaacgcct gcaacgcggc
    86701 gttgcgtcat tctcgctaca actggcagaa ggtcgggcag atctctacac ggaaatgcca
    86761 gtgaaagtca gtggctttaa acagccgata gatgatgcgg aatggaccat tacgactctg
    86821 acgcatactg tcagcccgga taacggtttt acgaccagtt tggagcttga agtgaagatt
    86881 gatgatttcg aaatggaatg attcttcgca atggagaact tttaagtttt caaaatggaa
    86941 taatgcggta tcattattgt gaatttagca aaaatgggga gaactcgaaa aatgatgatt
    87001 tgcccactgt gtggaagtgc cgcccatact cgcagcagtt ttcaggtatc ttcattgacc
    87061 aaagagcgtt acaaccagtg ccagaacatt aactgcagcc atacttttgt tacccatgaa
    87121 acttttgttc gttcgattgc aacgccaaaa gagtcaaatc cggttcagcc gcatccaatg
    87181 aaatcaggac aggtggcgct ctctctttga cgctgccgcc attttgtcgc catcgttaaa
    87241 aaacagtgtt tctaacatca tgattttaaa cagcttaaat ttcaggcaac aaaaaaccca
    87301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
    87361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
    87421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
    87481 tccctgccag cgtcagcagg acgttggcga ttgcataggt gcccgcatag cccagcgccg
    87541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
    87601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
    87661 aacagataac cactggcaca agactgacaa tcaatccggc aatcaacatc tgaccgccaa
    87721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
    87781 ccattaagcc gaactctttc accatgctta atgcaccctg cggaatgtaa ccgaaggtcg
    87841 ggtggttagc gcgcataaat cccagcataa ttccggcgaa taacaacccg gcagcgttcc
    87901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
    87961 caaagaaggc gcagaatgcc agcaagtcag tgacctggct gtgaatcgag ataaagccga
    88021 tgcgatcggc gatggttttt acgcgacggg catcgccgct gacttgtaaa acgtcacctt
    88081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
    88141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttgcccacg gcgttatggt
    88201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
    88261 ctttgccgtt acggaagctg ggatcgagtc gtgcgtgggc gtcgggatag cctaccaacg
    88321 ctatttcatc tcccatttgt agcacagcat caccgtctgg attcgccaga atcccgttac
    88381 gtcgaatacg ttcaatgtag cagccagttt gtcgataaat acccagttca cgcagatttt
    88441 tgccgtcggt ccaggccacc agctccgggc cgacgcggta ggcgcggatc accggtaaat
    88501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
    88561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
    88621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
    88681 gcctgctttc catgccggaa tgacgcagtg tatcgccagc accgaccaga accggtgtcg
    88741 acgtcataga gccagctaac ataccggccg tcaggccaat atcccagcca aacagcttac
    88801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
    88861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
    88921 aaataaacag cataaagcca agattaagcg cgtcggtgtt aatgctgaaa tgttgttggc
    88981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
    89041 gtaatttccc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
    89101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
    89161 tgttgaaaga aatggtaatt tacgataatg ttttttacca gaattcaggg cgcagattca
    89221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
    89281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
    89341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
    89401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
    89461 tgtctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
    89521 ggtttgctat ttttcattct tcctggtgca gttgccagct tcttttcaca tcgtagagaa
    89581 gtcctgaaac ctctgtttgg cgcaatgctg gcggcaccct gttcgatgct cattatgcgg
    89641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttatt aagtgcggtg
    89701 ttctggtgtg cgctgggggc attgtgtttc ttatttatca gtagtttgtt taaaccacag
    89761 cacagaaaaa atcaataaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
    89821 ttctctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
    89881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
    89941 ccttccgcac gaatatctac atactgatac tggaaatcat cgcgctcatt gctcaatttc
    90001 tcggccagat cttttgcacg cacacagtaa gggcaacccg gacgaccaaa aataacggtt
    90061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
    90121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaatgca
    90181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
    90241 aattgcctaa aagcgtgttg atactggaat ttatcggaat gatgttgctg gcggtggcgc
    90301 tgctgtcggt aagcgactcc ctgtcgctac ctgagccatt ttctcggcca gaagtgcaga
    90361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
    90421 aggtggcaaa acgtcttgcc ccacagctga tgaaccatcc accgcaatat tcacgttcag
    90481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
    90541 catttcactg atgaacccat ttccgaagcg cagcgtgagg cgattattaa cagcgcccgt
    90601 gcgacgtcca gttccagttt tttgcagtgc agtagcatta ttcgcattac cgacaaagcg
    90661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
    90721 ttttgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
    90781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc acagaatgca
    90841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
    90901 attgaagcgg tgacggaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
    90961 tgccttggct ggcctgcgga taatccggat cttaagccgc gtttaccggc ctccattttg
    91021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    91081 ctggcggaat attacctcac ccgtggcagc aataatcgcc gggatacctg gagcgatcat
    91141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    91201 ggttgggcga cgcgctaaaa ctgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    91261 ctgacagaga ggtgcagagt gaaaattgcc atattgtccc gggatggaac gctctattcg
    91321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    91381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    91441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    91501 gcggtactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    91561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    91621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    91681 ggtgcgccgc tggtggtcaa gctggttgaa ggcacgcagg gaattggtgt cgtgctggcg
    91741 gagacgcgtc aggcggccga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    91801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggc
    91861 gatgaagtag tcgctgcgat tgaacggcga gcgaaagagg gcgattttcg ttccaatttg
    91921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    91981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    92041 gggccgttgg tgatggaagt gaatgcgtcg ccagggctgg aaggaataga aaaaaccacc
    92101 ggtatcgaca tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    92161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    92221 ttgcgtaagc tgtgccggtc tttttatcgg aagaggttgt acaaaattat gacatcgctg
    92281 gtcgttcctg gtctggatac gttgcgtcaa tggctcgatg acctggggat gagttttttt
    92341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    92401 gatgctaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    92461 ccttcagccg tattgccgct ggcggcggat ttatcagcca tcaatgccag ttcgctgacc
    92521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    92581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    92641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    92701 gatgaagggc aaaataacgt taccgaaaac tatttcctcc actgataact cctttcgagc
    92761 acgcagtcgc tgccgcagtg actgcgtgcc gcaaaattat ccgctgtttt taacctttta
    92821 ttaaagatta attcacttct cttgtgccga tttggcttta tcacatagag caaatacgca
    92881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    92941 tattcttgcg atgcttttat atagtgagca gtgcaggccg ggagagagtt ctcttttctt
    93001 acaccgcgcc gataaaaata tgcacgttta ttgcatatct ttcagtgtga caacttttgt
    93061 tcgtttgtta acgaattttc agaaggaaag agatatgacc gccttaaata aaaaatggct
    93121 atcgggtctg gttgcggggg ctctgatggc cgtctctgtc agcacgctcg cggctgaaca
    93181 aaaaacactc cacatttata actggtctga ttatatcgcc ccggacacgg tggccaactt
    93241 tgaaaaagaa accggtatta aagtcgtcta cgatgttttc gattctaacg aagtactgga
    93301 aggcaaatta atggcgggga gcaccggctt tgatctggtg gtgccgtcag caagctttct
    93361 tgagcgccag ctgacagcgg gagttttcca gccgctggat aaaagcaaat tgccggagtg
    93421 gaagaatctc gatccggagc tgctgaagtt ggtcgccaaa cacgatcccg acaataaatt
    93481 tgctatgccc tatatgtggg cgacgaccgg gattggctat aacgtcgata aagttaaagc
    93541 ggtgctgggc gaaaacgcgc ccgtcgatag ctgggacttg atcctcaaac ctgaaaatct
    93601 ggaaaaactg aaaagctgcg gtgtctcttt cctcgatgcg ccagaagaag tgtttgctac
    93661 cgtgctgaat tatctcggca aagatcctaa cagcactaaa gcggatgatt acaccggacc
    93721 ggctaccgat ctgctgttaa agctgcgccc gaacattcgt tatttccatt catcccaata
    93781 catcaatgat ctggcgaatg gtgacatctg tgtcgctatt ggctgggcgg gcgatgtctg
    93841 gcaggcgtca aaccgcgcga aggaagcgaa gaatggcgtg aatgtctcgt tctcgattcc
    93901 aaaagaaggg gcgatggcgt tctttgatgt attcgccatg cctgcggatg ccaaaaacaa
    93961 agacgaagcc tatcagttcc tgaattacct gctgcgtcct gatgtggtgg cacatatctc
    94021 tgaccatgtg ttctatgcta acgccaataa agcagccacg ccgctggtga gtgcggaagt
    94081 ccgtgataac ccgggtattt atccgcctgc ggatgttcgt gcgaagctgt tcactctgaa
    94141 agtgcaggac ccgaaaatcg accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg
    94201 aaaataatcc gcagccgtag atgccggacg ggcgcaccac acccgccggc attcgcacca
    94261 tcatggtgcg cttgcacaca ttcaatgccg gagagcagcc gtgaatgacg ctatccctcg
    94321 cccgcaggcg aaaacccgta aggcgctgac gccgctatta gaaatccgta acctgaccaa
    94381 atcctacgat ggtcaacatg cggtggatga tgtcagcctg accatctaca aaggtgaaat
    94441 cttcgcgctg ctgggcgcat ccggctgtgg caagtccacg ctgctgcgta tgctggcagg
    94501 ttttgaacaa ccttcggccg ggcagattat gctcgatggc gtcgatttgt cacaggtgcc
    94561 gccttacctg cgccccatca atatgatgtt tcagtcttac gcgctgtttc cgcatatgac
    94621 cgtggaacag aacatcgctt ttggcctgaa acaggacaaa ctaccgaaag cggaaattgc
    94681 cagccgggtc aatgagatgc ttgggctggt gcatatgcag gagttcgcca aacgcaaacc
    94741 gcatcagctt tccggtggtc agcgtcagcg tgtggccctg gccagaagcc ttgctaagcg
    94801 cccgaaacta ttactgctcg atgagccgat gggcgcgctg gataaaaagc tgcgtgaccg
    94861 gatgcagctt gaagtggtgg atattctgga gcgcgtcggt gtgacctgcg taatggttac
    94921 tcacgatcag gaagaggcga tgaccatggc ggggcgtatc gccattatga atcgtgggaa
    94981 atttgtccag attggcgagc cggaagagat ctacgagcat ccgaccactc gctacagcgc
    95041 tgaatttatc ggttcggtta atgtctttga aggtgtactc aaagagcgtc aggaagatgg
    95101 tctggtactt tattcgccgg gactggtgca tccactgaaa gtcgatgtgg atgcttcggt
    95161 tgtcgataac gtgccggtgc atgtggcgct tcgcccggaa aaaatcatgc tttgcgagga
    95221 accgcccgcc aatggttgta acttcgcggt gggggaggtg atacacattg cctatctcgg
    95281 cgatctttcg gtgtatcacg ttcgtctgaa aagtgggcag atgattagcg cccagctaca
    95341 aaacgcccat cgtcatcgta aagggttacc gacctggggc gacgaagtgc gtttgtgctg
    95401 ggaagtggac agctgtgtgg tgctgacggt ttaaggagca aatatgagta cacttgaacc
    95461 tgctgcccag ccaaaaccct ttaagctgtg gttgtcgcag ctgcaaatga aacatgggcg
    95521 caaactggtt attgcgttgc catatatctg gttgatcttg ctgtttctgc tgccatttct
    95581 gatcgtcttt aaaataagcc tggcggagat ggcgcgcgct attccacctt ataccgaatt
    95641 gatggagtgg gctgacgggc aactttctat cactcttaat ctcggtaatt ttctgcaact
    95701 gaccgacgat ccgctctatt ttgatgctta tctccagtcg ttacaggtgg cggcgatttc
    95761 gactatttgc tgtttactga tcggctatcc gctggcgtgg gcggtggcgc acagtaagcc
    95821 ttcgacccgt aatatcttat tactactggt gatcctgccg tcgtggacct cgtttctgat
    95881 tcgtgtctac gcctggatgg gaatattaaa aaacaacggt gtgctgaata attttctgct
    95941 gtggctgggg gttatcgatc aaccgctgac cattctgcat accaactttg cggtttatat
    96001 tggcattgtt tacgcctacg tgccgtttat ggtgctgccg atttacaccg cgttgattcg
    96061 tattgattat tcgctggtgg aagcggcact ggatctcggt gcacgaccgc tgaaaacgtt
    96121 ctttactgtg atcgtgccgc tgactaaagg tgggattatt gccggatcga tgctggtgtt
    96181 tatcccggct gtgggcgagt ttgtgatccc ggaactgctc ggtggcccgg acagcatcat
    96241 gatcgggcgc gtgctgtggc aggagttctt taacaaccgc gactggccgg tggcctcagc
    96301 ggtagcgatc atcatgttgc tgctgctaat tatgccgata atgtggttcc acaaacatca
    96361 gcaaaaaagc gtgggagaac acggatgaat aatttaccgg tggttcgttc gccctggcgg
    96421 attgtgattt tgctgttggg cttcaccttt ctctacgcgc caatgctgat gcttgttatc
    96481 tattcgttta acagctcgaa gctagtgacg gtgtgggccg gctggtcaac gcgctggtat
    96541 ggcgaattat tgcgcgatga cgcgatgatg agtgcggttg gtttaagtct gacaattgcg
    96601 gcctgtgcgg cgacggcggc ggcgatcctc gggactattg cggcggtcgt gctggtgcgc
    96661 tttggcaggt ttcgcggatc aaatggtttt gccttcatga tcaccgcacc gctggtaatg
    96721 ccagatgtca tcacgggttt gtcgctgttg ttgttattcg tcgcgcttgc tcatgctatt
    96781 ggctggcctg cggaccgcgg tatgctcacc atctggctgg cgcatgtcac tttctgtacg
    96841 gcttatgtgg cagtcgttat ttcgtcgcgt ctgcgggaac tggatcgctc gatagaagaa
    96901 gccgcgatgg atctcggtgc gacgccgctg aaagtgtttt ttgtcattac gctaccgatg
    96961 atcatgcccg cgatcatttc tggctggtta ctggctttta ctttgtcgct tgatgatctg
    97021 gtgatcgcca gctttgtttc cgggccggga gccaccactt taccgatgct ggtcttttcc
    97081 agcgtgcgga tgggggtgaa tccggaaatc aacgccctgg caacattgat cctcggcgcg
    97141 gtcggaattg tcggatttat cgcctggtat ctgatggcgc gcgcagaaaa acagcggata
    97201 cgcgacatcc agcgtgcaag acgtggctga agacactaaa atttgccaac ctggctacat
    97261 aatgccgcgc atgtcgcggc attgttttca tggaagacga aacgttggga ttttttaaga
    97321 aaacatcttc atctcatgct cgcctgaatg tgcctgcgct ggtgcaggtg gcggcgctcg
    97381 ccattattat gatccgtggc ctcgacgtgc tgatgatttt caatacgctg ggcgtgcgcg
    97441 gtattggcga gttcattcat cgcagcgtac aaacctggag tttaacgctg gtctttttaa
    97501 gcagtctggt gctggttttt attgagatct ggtgtgcgtt ttcactggtg aaagggcgtc
    97561 gctgggcgcg ctggctgtat ctgttgacac aaatcacagc cgcaagttac ttgtgggcgg
    97621 cctcgctggg gtatggttat ccggagctgt tcagcattcc cggtgaatca aaacgtgaaa
    97681 tcttccatag cctgatgctg cagaagctgc cggatatgct catcctgatg ctgctgttcg
    97741 ttccctcgac cagtcggcgg ttcttccagt tgcaataatg tgtataatcg tcgcccttga
    97801 tgatgtgaag gtcaatgtat gcagtgcgca ctttacgacg cgggtcgctg tcgttcctgt
    97861 cagtggataa cgcagccgat tccagagcaa ctctccgcta aagccgccga tcttaaaaat
    97921 ctgctcgccg attttccggt tgaggaatgg tgtgcgccgg tgtcaggccc ggaacaaggg
    97981 tttcgtaata aagccaaaat ggtggtgagt ggtagcgttg aaaaaccact gctcggtatg
    98041 ctgcatcgag atggtacacc ggaagacctt tgtgactgcc cgctttatcc agcctcattt
    98101 gcgcccgttt ttgcggcgct aaaaccgttt atcgcccgtg cggggttaac gccctacaac
    98161 gtggcgcgta aacgtggtga actgaaatac attctgctga ctgaaagcca gagcgatggc
    98221 ggcatgatgc tgcgttttgt actgcgttct gataccaaac tggcgcaact gcgtaaggcg
    98281 ctgccgtggt tacaggaaca attaccgcag ctgaaagtta ttaccgtcaa tattcagccg
    98341 gtacatatgg cgattatgga aggggagacg gagatctacc tgaccgaaca acaggcactg
    98401 gcagagcgtt ttaatgacgt accgctgtgg atccgtccgc aaagtttctt ccagactaat
    98461 ccggcggtcg ccagccagct gtacgccacc gcgcgcgact gggtacgaca gctgccggtt
    98521 aaacatatgt gggatctgtt ctgtggtgtg ggtggctttg gtttacactg cgcgacgcct
    98581 gacatgcagt taaccgggat cgaaattgca ccagaggcca ttgcctgtgc gaagcagtca
    98641 gccgctgaac tgggcttaac gcgtttgcaa tttcaggcgc tggactctac gcagtttgcc
    98701 accgctcagg gggaagtgcc ggagctggtg ctggttaacc cgccgcgccg cggcatcggt
    98761 aaaccgctgt gtgattatct ctcaacgatg gcaccgcgtt ttattatcta ctccagctgt
    98821 aacgcccaaa ctatggcgaa agatatccgc gaactgcctg gttatcgcat cgaacgggta
    98881 cagctttttg atatgttccc gcacaccgcg cactatgaag tgctgacgct actggtgaag
    98941 caataaaaaa gccgcaggtg cggctttctg aatcttactg tgggaaccac tggtcattga
    99001 ttttctgata tgtaccgtca gctttaattg ctgccagcgc gttattcagt ttttccagca
    99061 gagctttgtt atccggacgt acagcgatac ccaggccggt gccgaagtat tgcggatcgg
    99121 tcactttttc agtagcaacg cccagttgcg gattggtttt cagccattcg tttaccactg
    99181 cggtgtcacc aaatacgcca tcaatacggc catttttcag atcgataaag gcattctgat
    99241 aactgtcata ggagacagtt ttcacttccg ggtgctgatc ctgaatatat ttctggtgcg
    99301 tggtgccgtt ttccatccca atacgtttgc ctttcagatc ggcaaacgtt ttgtaggtat
    99361 cttttttggc aatcacgacg gctgagtttt catagtaggg cgtggtaaac gatacctgtt
    99421 tgctacgctc cggcgtgata tccattccgg agattacggc gtcatatttt ctgaatttca
    99481 gtgacgggat caggctgtcg aaagcgtgat tagtaaaagt acattctgcc tgcatttgtt
    99541 tgcacaaggc ttttgccagg tcgatatcaa agccgacaat ctcattatta gcacctatag
    99601 attcaaaggg tggataggtg gctgaaacgc caaaattgat tttctctgcg gcagaagcac
    99661 cgaaagtaaa agaagcaagt aatgcggcaa gaactaactt tttcatgatg gaactcccgt
    99721 ctgtcaatct tatgattttt ggccgtgtct gcggcatggg ataacaatgc catcaagtga
    99781 atttatatgc aataaatatg agtaaataat ttatatgaaa taaaaaaaga cggacagctt
    99841 agtgggttgt ccgtcttcat aataagaatt tatgcactat gtaggccgga taaggcgtcc
    99901 acgccgcatc cggcatcagc atgcagctac attaattccg ccgttcgaat gccagtgctt
    99961 tacgctcgat cagacgcatc atcagcgtca gcaggccgtt gacgaccagg taaataatcc
   100021 ccgccgcgcc aaataccatt acatcgtagg tgcgtccgta caacaactgg ctgtatccca
   100081 tcacttccat gagcgtaatg gtgtaagcca gagaggtact tttgaacacc agcaccactt
   100141 cgttggaata agaagagagc gagcgtttaa acgcgtacgg tagcaggatc gccagcgtat
   100201 cttttttgct cattcccagg gcgctacagg actgccattg accttccggg atcgcgcgaa
   100261 tcgcaccgta aaacagctgc gtggtgtacg ccgcactgtt cagcgacaac gcaatcagcg
   100321 cacataacca cggttctgac aacaaatgcc acagtgccgg atactcctgc aacgttggga
   100381 actggcccgg tccgtaataa atcaggaaga tctgcaccag cagcggcgta ccggtaaaca
   100441 gcgtgatata gccccgcacc agccacacca gcaccggcgt tttcagcgtc aggatgatgg
   100501 taaaaatcaa tgccagaatc agtgccacaa tcagcgaggc aacggttagc gtcaggctgg
   100561 tgtgcagccc tttcatcagt tcgggtaaat actcaaacat tagctgggcc tccgctcaaa
   100621 acgtgtcgcg cgcaggtcaa tgcgtttgag aatgtactga ctgagcaggg tgatcaccag
   100681 gtaaatcacc gccgccacaa tgtaccaggt aaacggttcc tgtgtacggg tagcgatgct
   100741 tttggtttgt agcattaaat cattcacact aatcaaactg accagcgcgg tatctttcag
   100801 cagcaccagc cactggttac cgagacccgg cagcgcatga cgccacatct gcggcatcac
   100861 cagacggaaa aagatagccg atttcgacag ccccagcgcc tgaccggatt cccactgacc
   100921 caccggcacc gcttttaatg caccacgcag cgtttgcgag gcataggcgg catacagcag
   100981 tgacagagcg atgacaccac aaaggaacgg gctaacgtcg aagttctcaa tgtccatctg
   101041 caccgggatc tgcacgaacc caagattgat agtgaagcca tccgaaagcg tcagcagcag
   101101 ctgcgaggag ccaaaataga taaacagcac caccagaatt tctggcaggc cacgcagaat
   101161 ggttaccagc gctgaacctg cccacgcgac aggacgccat ttcgcagact cccataccgc
   101221 aaagaacatc gccagcgcca gcccgacaat caatgcacaa acggcaaggc cgacggtcat
   101281 cccggcggcg cttgctaaag gaaaaaattc attcatcagg aattacttct ggaaccattt
   101341 gttgtagatg gtttcgtaag tgccatcttt cttcactttt tccagcgcag tgttgagttt
   101401 ctgctgcagt tcagtgttgc cctggcgtac tgcgatgccg aggccagtac cgaagtaatc
   101461 tttatcggtc actttatcgc ccactgccgc cagtttcggg ttatctttca gccattctgt
   101521 taccactgcg gtgtcaccaa atacgctgtc gatacgaccg ttttgcagat ccaattttgc
   101581 gttctggtaa ctgtcatatg gcacggtagt gatttccgga tgcttatcca taatgaactt
   101641 ctggtgtgtc gtcccgttct gaacgccgac ttttttgcct ttcagctgat caacactggt
   101701 gtatttgcct tgctgaccca caaacagggc agagttgtca tagtacgggg tggtaaacag
   101761 cacctgcttt tcacgctccg gagtgatatc catgcccgcc atcactgctt ctacgcgacg
   101821 gaatttcagg cttgggatca ggctgtcaaa cgcctggtta gagaaagtgc aggttgcatc
   101881 aatctcttta cacaatgcct gcgccaggtc gacatcaaaa ccaacgatct ggttgtttgc
   101941 atcaatcgat tcaaacggag gataggaggc ttcggtggca aaacgaatgg tttcggctgc
   102001 tgtggcggaa agactaaaac ctgcaattaa cgcggcaatc agaacttttt tcattgttgt
   102061 tatcccgaat cttagtgaga gagatagttt ttaaatgctt cggtttgcgg ctcggtaaag
   102121 cagctcgcgt cgccttgttc tacgatatga ccgttttcca tatacaccac tcggctggcg
   102181 gttttacgcg ccacttcaac ttcgtgggtg acgatcacct gggtaatatt cgtttctgcc
   102241 agctcacgaa tgatgctgac gatttgtgcc gtaatttcgg ggtccagtgc ggcggtcggt
   102301 tcatcgaaca gcagtacctg cggttccatc atcaacgcac gggcaatagc aacacgctgc
   102361 tgctgacctc cagaaagatg cagcgggtaa cgatcgctat aaggtttgag acgcagacgt
   102421 tccagcagtt tttctgcacg ggccagcgcc tgatctttac tcaaccccag tacacgacag
   102481 ggcgcttcaa tcaggttttg ctgcacggtc agatgcggcc acaggttgta ttgctgaaac
   102541 accatgccaa cgttacgacg caaatcgcga atcgctttgt cagagggggt tttggtgaaa
   102601 tcgaaatggt tgcctgcaat gttgagcgta ccggagcgcg gcatctcaag cagattgagt
   102661 acacgcagca gcgagctttt acccgcgccg ctggggccaa gtaacaccag cgtttcgccc
   102721 tgtgggcaat ccagcgtgat atcgaacagc gcctgatgcg cgccgtagaa gcaattaatg
   102781 ccgtttaatt gaatactcat tgacactcgt atactggcag tctgatagct attgaggtcg
   102841 aagatagtac ctttgacaga ataattatgc aatatttctg ctttaaaagt taaaagcaaa
   102901 gcgcattatt caataaacat agcacaaaat aacgggggcg gtggtcggcg agcataaatg
   102961 tcggcattcc tcacgaaatg ccggacaatt tacggggttt attggttgat caagacgtta
   103021 gcgattctcg atggactgac ggagcgtacc cgccgtggca tgaacgctac cgcctaagta
   103081 gcgcacatcg tcgatgaccc aacactggcc ttcctgaatc attaacactt catcctgcca
   103141 gccctggtca ccctgcttga gatccacgcg caatggaatg ttacgggcat cacgattagg
   103201 gatagtagat gcactggcaa cgtgggcgct gtctggcaag gtggttcgac tggagaacgg
   103261 atcgctgctt aacaattcac gatgactatt atcgcggctg gcatcgctaa gtagggttgc
   103321 cagtttgtca ctcaaatagg ggcgcagagc ggtaatgtca ttgccgcgat gttgaatgcg
   103381 gtagtcatag aactgttgag ccacgctatc aggccccccc tggacacagg aaccgatgcg
   103441 ggggccgtta tctttataag ctggcgtgac ggtggtacag gcactgagta gcagtgcgca
   103501 agggataagc atggtcaatt tgctatagcg catgatgatt tccttataag cgaacacctt
   103561 gaaagcgttc tacgataata atgatatcct ttcaataata gcgtatcagt ccgataatgc
   103621 tttgaagatc gaaggcttag caaacaagga gatcgatcat gcaattttct acaaccccaa
   103681 ctctggaagg ccagaccatc gttgaatatt gcggtgtggt gactggcgaa gcgattttag
   103741 gcgcgaatat tttccgtgat ttctttgccg gtatccgcga tatcgttggc ggacgctccg
   103801 gtgcctacga aaaagaactg cgtaaagcgc gggagattgc ctttgaggag ctcggctccc
   103861 aggcgcgagc gctgggggct gatgccgtcg tcggtattga tatcgactac gaaacggtcg
   103921 ggcaaaacgg cagtatgctg atggttagcg tcagcggtac ggcggtgaaa acgcgtcgat
   103981 gagaagagtc ttctggctgg tcgctgccgc tctgttactg gcagggtgca caggcgaaaa
   104041 aggcattgtc gagaaagagg gatatcagct tgatacccga cgccaggcgc aggcggcgta
   104101 tccgcgcatt aaagtgctgg tgatccacta caccgcagat gattttgata gctcgctggc
   104161 gacactgacc gataagcagg tcagctcgca ttatctggtc cctgcggtgc caccgcgata
   104221 caacggtaaa ccgcgcatct ggcaactggt gccggaacaa gaactggcct ggcatgcggg
   104281 gattagcgcc tggcgcgggg caacgcgcct taacgacacc tctattggca ttgagctgga
   104341 aaaccgtggc tggcaaaaat cggcgggagt gaaatatttt gccccgtttg aaccggcaca
   104401 gattcaggcg cttattccgc tggcgaaaga tattattgcc cgttatcaca tcaagccgga
   104461 aaacgtagta gcacatgcgg atattgcgcc gcagcgcaaa gacgatccgg ggccattatt
   104521 cccctggcag caactggcac agcaggggat tggtgcatgg ccggatgcgc agcgggtgaa
   104581 cttttacctt gccgggcgcg cgccacacac accggtagag accgcgtcat tgctggagct
   104641 tttggcgcgt tatggttatg acgtaaagcc tgatatgact ccgcgcgagc agcggcgcgt
   104701 gattatggcg ttccagatgc atttccgccc gacgttatat aacggcgaag cggatgcaga
   104761 aactcaggcg attgccgaag cgttgctgga gaaatacggg caggattagc gcggcagttt
   104821 tccgtggtcg cgcagccagg cggcagtttt ctcgatacct tcatccaggg taaggactgg
   104881 ctgataacct agctcttcct gcgcgcgcgt aatatccagc gtaaagtcaa aattcaactt
   104941 ggagacgccg tagtgggtca gcggcggctc ttttgctgac ttgcggccta aacgctccat
   105001 gctgcgggcg atcatatcca gcatcgggta agggacggaa cgaatacgac agtcaatatt
   105061 caattcgtct atcagcttct gcacgatgct gcgcagtgtg cgatgctcgc cgttggtgat
   105121 gttgtacaca cgcccggaag gtagcttatc gcaggcttcc tggcttgcca gccacatggc
   105181 gtgcacggca ttttcatagt aggtcatatc gaccagcgca ctgccgccat gcggtaacag
   105241 aatactgccg tagtgatgca tcatatgcgc cagacgaggg ataaagactt tatcgtgcgg
   105301 tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt tgtggattcg cctgcgaaag
   105361 catattgatc acttcttcgc tggctgcttt gctgcgggca aactcgttgg cgaagcggtg
   105421 agggcgaaaa tcttctttaa tatcgcgatg gtggtgataa tcgaagtaca gggagggaga
   105481 agagatatga ataaagttac gcacacccca ggcgacagcc cattcaccca ggcggcgagt
   105541 ggcacgaacg ttagccagat cgaaagcctg ttgtgtcccc cagggcgagg taaagctgga
   105601 gcagtgccac agcgtatcaa tgcctgcgag catcacttta gcttgtgatg agaccagctc
   105661 ggtcagatcc gccggaacaa actctgcgcc cattttttcc agcaatttac ccattgcctc
   105721 gttgcgaccg gtcgctcgca cgctgatgcc tttctggcat aaaaactcta ccgcgtttcg
   105781 acctaagccg ctggtggcgc cggtaaccag taccttcata tcaatccact gttgttgaga
   105841 aaataacgtg cgcattcttc cgtgatttcc tccatgatgc aatgggaaac atgaaagaat
   105901 aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca gtctggcgat tcgttttgcc
   105961 attccgcgaa aaataaacag atgcgcgggg atcatcaata accagtaaaa caaccctggc
   106021 ataccgtgcg gatgccagaa tgcgcgaaca tcgatagtac gatagtcacc tttatcttcc
   106081 agggtaaaac acagtcgtcc cagccccggc gctttcatgc caaataacag cgtaagttgt
   106141 ttttgcggtt caacgacaat cactttccag ctatccaccg catctccggt ctgcaaatat
   106201 tcacgctccg ggcggccttt cgccagctta tgaccgatcg cgcggtccat caacgcccgg
   106261 gtttgccaca aaatattgcc aaagaaataa cgctctttac cgccgatttg attcaccacc
   106321 tgccataaag ccgcaaggct ggcggacgtt ttaacggtaa accccgcctg tttggcgaaa
   106381 taaccgtact ccggtcgcca gcgggcaaag gcctgagcgt cgtagcccca gtcgctggag
   106441 ttgaccagct tttcttcctc tttcaacgtg ctacgtaccg cgtcatcgaa agcgatcagc
   106501 cgttgtggga tgagcgcacg tagcgcggtg tcatccgcca gcagatcgtg tttcagcccc
   106561 tgaatcaacg ccctggcggt ggtgggcggt actgaagtaa tcacattaag aaaccacacc
   106621 gaaatccagc gggtggggag ggggatgggg agcaaccagc ggcgcttacc gctcaccgcc
   106681 ataaaatgtt caaactgttg ctgataactg agcacttctg gtccggcggc ttcgaagatg
   106741 cgatgttcgc tggctggatg gtctaacagc gccaccagat agtgcagcaa gttttccagc
   106801 gcgatgggcg tggtgcgtga acgaacccag cgtggcggcg ttaacactgg caggttgtag
   106861 accatatcgc gcatgacttc gaacgccgct gaacctgcac caacgattat tccggcacgc
   106921 aattcggtca ccgggacacc cgcttcacga agaatatccg ccgtagcctg acgggcgcgc
   106981 agatggtccg actgctcatg tggcggggcc tgcaacgaac tgagaaagat taattgcttt
   107041 accggtactt cgcgcagcgc atcgcggacg ttgagagcca cctggcgctc ctgagcgata
   107101 aaatcaccgc cttctcccat gctgtgcacc agaaaataga ccgtatcgat atcctgcaac
   107161 agggccggaa ggttatccgg ccagctgaga tcgactttat ggcaactgac atttgccagt
   107221 tgcagctttg caagcctgtc gacatgacgt gccgccgcca ggatctgatg cccctgctgg
   107281 ctgagtgttc gcaccagatg ctgaccaatg tagccactgg caccgagaac taaaatgcgt
   107341 tgcggcacgt ctccctcctt aacgcgccag gaatgcgcgc cagtgggcgg cgacttccgc
   107401 cagctgagcg cgcgagacgt caagatgcgt caccaggcgg acaatcggcg aggcgttaat
   107461 cagtacgttt ctcgctttca tgtattcgcc taacgcggca gcattttctt ccccgacgcg
   107521 aacaaacagc atattggtgt cctgacgcat cacatccgcg cctgcttcac gcaactgctc
   107581 cgccatccag gcggcgttgt cgtggtcttc ctgcaagcgc gcaacgttat ttttcagggc
   107641 atatattccg gcggcagcca gaatgccgga ctggcgcatc ccgccacctg tcattttccg
   107701 ccagcgaatg gcacgtttaa tgtaatcacg attaccgacg agtaatgaac cgactggcgt
   107761 cccaagacct ttcgacaggc aaatggtgaa cgaatcacaa tattgtgtga tctctttcag
   107821 ttcgcagccg taagccacca cagcattaaa gatgcgcgca ccgtccacat gcagcgccag
   107881 attgcgctcg cgggtaaatt cccatgcttc tttgaggtat tcacgcggca gcactttgcc
   107941 gttgtgagtg ttttccagac tgagtaattt ggtgcgggcg aaatggatat cgtcgggttt
   108001 gattttcatc gccactttat ccagcggtag cgtgccgtcg gcagccgcgt cgatgggttg
   108061 cggttgaata ctgcccagca ccgctgcgcc accggcttca aacagatagt tgtgtgcggc
   108121 ctgaccgaca atatactctt cgccgcgttc gcagtgactg agcagagcga ccaggttagc
   108181 ctgggtgccg gtcggcagaa aaatggcggc ttctttaccg gaaagctctg ccgcatagtc
   108241 ctgcagagca ttaacggtag ggtcgtctcc gtaaacgtcg tccccaaccg gggcggccat
   108301 cattgcttcg agcatggcgc ggctcggtcg ggtaacggta tcactgcgta aatcaatcat
   108361 ggcatgtcct tattatgacg ggaaatgcca ccctttttac cttagccagt tcgttttcgc
   108421 cagttcgatc acttcatcac cgcgtccgct gatgattgcg cgcaacatat acaagctaaa
   108481 tcctttggcc tgttcaagtt tgatctgcgg tggaatggct aactcttctt tggctactac
   108541 cacatccacc aacaccggac catcgatgga aaaggcgcgt tgcagggctt catcgacttc
   108601 agaggctttt tccacgcgaa tacccgtaat tccgcacgct tcggcaatgc gggcaaagtt
   108661 ggtgtcgtgc agctcagtac cgtctgtcag gtagcctccg gctttcatct ccatcgccac
   108721 aaagcccagc acgctgttat taaagacgat aattttcact ggcaatttca tctgcattac
   108781 tgagaggaaa tcgcccatca acatactgaa accgccatcg ccgcacatgg cgaccacctg
   108841 acgttccggc tcggttgcct gcgcacccag cgcctgcggc atggcgttag ccatcgaacc
   108901 gtggttaaac gaacctaaca gacgacgctt gccgttcatt ttcagataac gtgccgccca
   108961 caccgttggc gtaccaacgt cacaggtaaa gatggcgtca tcggcggcaa aatgactaat
   109021 ttgctgcgcc agatattgcg ggtgaatggc tttctcgctc ggtttagcta aatcatccag
   109081 ccctttgcgg gcgtcgcggt aatcttccag cgctttatcc agaaacttgc gatcggtttt
   109141 ttcttccacc agtggcagca atgcccgcag ggttgatttg atatcgccga ccagcgccat
   109201 atctaccttg ctatgcgcgc cgatgctggc tgggttgata tcaatctgaa taattttggc
   109261 atcggtcggg tagaacgcac ggtagggaaa ctgagtaccg agcagcacta acgtatcggc
   109321 gttcatcatg gtatggaaac ctgacgaaaa gccgattaat cccgtcattc caacatcata
   109381 tggattatcg tattcgacat gttctttacc gcgcagggca tgaactatag gcgctttaat
   109441 tttcccggca aactcaacta actctttatg cgcccccgca cagccgctgc cacacatcag
   109501 ggcgatattg ctggaataac gcagcagttg cgccagtttg cgtaactctt cttcttccgg
   109561 tgttactacc ggctgtggcg catgatacca gtgggtagtt gccccttctg gcgcaggttt
   109621 taacgccacg tcgcccggta acacaacaac ggaaacgcca cggttaagca ccgctttgcg
   109681 catagcaatt gccagcactt gtgggatctg ctctgggctg gaaaccagct cgcaatagtg
   109741 actacattcg cggaatagct cttgtgggtg ggtttcctgg aaatagccgc tgccaatttc
   109801 gctggaggga atatgagcgg caatcgccag taccggaacg tgattgcggt ggcaatcgaa
   109861 caggccgttg attaagtgca ggttgccggg accgcacgat ccggcacaga ccgccagttc
   109921 tccgctaagt tgtgcttcag cgccagcggc aaacgccgcc acttcttcat gccgggtgga
   109981 catccactcg atggtgccca tgcgattaag actgtcacta agaccgttca gagagtcgcc
   110041 cgtgactccc cagatgcgtt tcacgcctgc cgattcgagt gttttggcga tataagctgc
   110101 aaccgtttgt ttcatggttc tccatctcct gaatgtgata acggtaacaa gtttagttca
   110161 tctgacggag ggggaaggga tgggagaaaa aggaggcact aacggttaaa tagcccgatg
   110221 aaaggaatat catcgggcat caggcgatta tgcgagaacc aaatccccct gcggatggca
   110281 ggagcaggcc agtacgtaac cttcagcgat ttcggcgtcg gtcagcgtca ttgtgctgct
   110341 caccgtatat tcaccggaaa tcactttcgt cttacagcag ccgcaaacac ccgcacggca
   110401 ggcggcgaca accggaacgt tattgctttc cagcgcctcc agtagcgtgg tgccaaccgg
   110461 agcgtaaaat tctcgtgccg gttgcagttt ggtgaatttc agaccgctgg tcgccgcttc
   110521 cgctactggg gtgaagaatt tttctttaaa gaaacgcgtc acgccgagcg ctttcacttc
   110581 ctgctctacc caatccatat acggagcagg gccacaggtc atcacggtac gtgaggctaa
   110641 atcagggaca cctgccagca gttcgcgagt gagacgacca gcgataaagc cttcggtaac
   110701 gttattttcc gccaccagcg ttaccggata gttacgccac tcatcggcga aaatcacatc
   110761 ctgcggtgta cgcacgttgt agatcacctg cacatcggcc tgtggacggt tcttcgccag
   110821 ccagcgacgc atcgacataa tcggcgtgac gccgcagcct gccgccagca acaggaattt
   110881 atcttctgct ttatcgtcgc aggtaaattc ccccatcgcg tccgaaagcc agagataatc
   110941 accgcgtttt acatcgcgcg tcagccaccg ggagccgaca ccgtcatcaa tccgccgcac
   111001 ggtcagggtg atgtattcac tcacgcctgg cgtggaggaa atggtgtaag cacgcagcgt
   111061 ttccgctgag tttcgcacgc tgaccagcgc atattgcccg gcgcgatatg ggtagtaatc
   111121 gtggcaaatc agggaaatcg tccacacatc cggcgtttct tgcgtaatgt gatgaacctg
   111181 catccgccac gggcattgat tcgttggcat cgtcatcgac aaactcctta cgcgctcaac
   111241 agttgcttca tgtcttcttc aacagtggtg atagaacgca ggccgaattt ctcgttcagc
   111301 accgccagca ggtctggtgt caggaaacca ggtgcagtcg ggccggtgac gatatttttc
   111361 acacccagag aaagcagcgt cagcagaatg acaatcgctt tctgttcaaa ccaggagagc
   111421 accagcgaca gcggcagatc gttgactccg cagcccagtt tctctgccag cgttaccgcc
   111481 agaataattg ctgagtaagc atcgttacat tgaccagcat ctaccagacg cggcagacct
   111541 tcgatatcgc caaactcaag tttattaaag cgatatttac cgcaagcgag ggtcaggatc
   111601 aggcagtcat ccggcacgct ggtggcgaaa tcggtgaaat agtgacgctc gccgcgtgcg
   111661 ccgtcacagc cgccaagcag gaagatatga cgcagttttt cacggctcac cagatcaatc
   111721 agcgtatcgg ctgcgccaag cagtgtctgg cgaccaaagc ccacggtgat caggtgcgga
   111781 atttcgctgt acgggaagcc cgccatctgt tgcgcttggg caatgactgc agagaaatct
   111841 tcgccatcca gatgacgcac acccggccag ccaacgatgc tgcgggtcca gatacgatcg
   111901 tcataagcgc ctacggttgg gtcgatgatg cagttcgagg tcatcacgat ggggcctggg
   111961 aaacgagcga actccacttg ctgattctgc cagccgctgc cgtagttacc gaccagatgc
   112021 ttgaatttac gcagctccgg gtaaccatgc gcaggcagca tttcgccgtg ggtgtagaca
   112081 ttaacgcccg tgccttcggt ctgttccagc aggttgtaga gatctttgag atcgtgaccg
   112141 gaaatcagaa tgcatttacc cgccgtcgct ttgacgttga cctgagttgg cgtcgggtga
   112201 ccgtatttac cggtttcgcc tgcgtccaga atgctcatca ctttgaagtt catctggccg
   112261 atttccattg aacactcaag aagcgcgttc atatcggcag gccaggtccc cagccacgcc
   112321 atgattttat ggtactgggc ataaatatcg ttgtcgtatt gaccgagaac atgcgcgtgt
   112381 tccatatagg ccgccgcacc tttcaggcca tacaggcaca gcagacgcag gccgagaatg
   112441 ttttcgccaa tcgccgcttt atctttgtta ggggtaaatt ctgctgcctg acgttgcagc
   112501 tcgccgagat cgtcgctcat cagttgcagg tcagccatcg ggttatcgac gcgggcgttg
   112561 gcatctacag ccaggcattg tgctttcagc gcctcgcgca gggcaatcgc ttcacgagcg
   112621 tagccgacaa tacgcggaga atcgaagtta acgttggtca gggttgagaa aaaggcacgt
   112681 ggcgcaaagc tgtctacatc gtggttgatg atgccgtatt cacgtgcttt cactgcccag
   112741 gccgaaagcc cttgcagcgc cgcgatgagt aaatcctgaa ggtcagaggt ttccgccgtt
   112801 ttaccacaca tcccctgcgc gtatgagcag ccgtttcctg ccggagtacg gatagtttgt
   112861 tcacattgca cacaaaacat gatcacacct tttaaagtta tatttaatat acatgtttaa
   112921 ggttaagacg cttaacgcgg ggataaaaga gatttttaat gcaactttaa gggagattga
   112981 tttagcgcaa ttttggcggc agagatctac cgccagagag gtattatgca gagaaaaagg
   113041 cgatgagaat cggcactaac agactaagaa taaaaccgtg aacaatcgcc gccgggacca
   113101 tatccagccc gcctgtacgt tgaagaacgg gcagggtgaa atccatcgac gtcgcaccgc
   113161 ataagcccag tgcagtagag cggctacggc gaatcagccc agggattagc ataatagcaa
   113221 tcagctcacg ggccagatca ttaaaaaacg ccgcgctccc gattaccgga ccaaaagatt
   113281 cggtcaataa aataccggaa agagaatacc agccgaaacc ggaggccatt gccagcgcgg
   113341 tattgatggg aagatcaaga ataaaggcgt taattaaacc accaattaat gaactggcaa
   113401 ccaccaccac ggcgacaatc attccccggc gattaaggac aatctgcttt aaggtcatgc
   113461 cattattgcg caactgaata ccgacgagga aaagtagcag aattaaggtg tattcactgg
   113521 cttcggtcgc gtgttgtaag aaagccagtc cactcagacc aatggcaaaa ccaatcacta
   113581 ctacgccgca cagttttagc gactccagcg ccatcgcaat acgcgacggg agtttttctt
   113641 gatgatggtg gttgcgccat ggcaggccgc gctccagcca catcagggcg gcaatattac
   113701 acagtaaaat aacggtaata ctgacggcag aataatgcag aattgccaac aagttactgg
   113761 cgaggttatc gagaaacgcc agactgatac ccataaaaaa gagaataagg taaaccatcc
   113821 aacttaatag ctgattaata actcttaatg cagctttttg gcgaagcgga atgaggtaac
   113881 ccacaatcag gggaaccaga atgattaaca gcccagaaaa catgaaaaac cagtccttgc
   113941 aaagaagatt cgaaatgcgc gatgacacac tactgaaagc ggaaggacga gtaaagttgc
   114001 aattaaaagg aaatgttatg cataaggatc ggtagggtat tcattttcat ttaaagatat
   114061 tcttgcgctt taattacaaa ccgcaccggt gttggtggcg tcaaaatcgc cgaggcgttc
   114121 cctgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgttttac agggatgtta
   114181 cctcgcgccc gacccggtag ccgtaaggga taagtcgagg gcaccgcgca gcggcgattt
   114241 tgttcgccag agcccggggg tgcagggggc ggcggcgatt ggccgccccc tgcacgctcc
   114301 ttgcgccagt ggcaatatgt ggcttagctc atgaaaggag cgcaacaaga tgatgaatca
   114361 acatataaca acatcttaaa aaaaggcctg acattgcgcc aggccttctg cgttaattaa
   114421 tcgcgttttt ccagtagagt ccggtaaatc agaccaccga taataccgcc gacaattggc
   114481 accacccaga agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgca
   114541 gtactgcgcg ccgggttaac ggaagtgtta gtcaccggaa tactgattaa gtgaatcagg
   114601 gttaaggcca gaccaatggc aattggcgca aaaccagcag gagcgaattt gtcagttgcg
   114661 ccgtggatca ccagcaggaa acctgcactc aataccagtt caactaccag cgcggaaagc
   114721 atggaataac cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc
   114781 gcgtcaaaac ccgttttacc actggcaatt aaatacagca gcgccgccgc aacaataccg
   114841 ccgacaacct gggcaattac gtagccaacg acttcttttg ccgggaaacg tccgccagcc
   114901 cataaaccaa tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcaaag
   114961 gccattgtca gaacggtcag accgaacgcc aacgccacgc cggcaaaacc aatgcctaat
   115021 tccgggaagc ctgcggccag tacggcacta ccacagccgc caaaaacaag ccagaaagta
   115081 ccaaaacatt cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt
   115141 gaaaaatatg gtcgttttat agggtcgtcg taaaaaagtg acgacggaaa tgatgcgcgg
   115201 ctattttaaa aacgaaggca agtcattcac cagataaata aatccagtaa atttgattta
   115261 aggcaacagc gggttgcccc atatagtcat ttgtctgatt gacagtgtag tgcaagcgaa
   115321 agatttaatc ctttaggcgt aataaaaaat aatttatcat gctaattatt tgattttgtt
   115381 gtttttgcag acttgtcagc aagagggagt ataacgcgat tattcgctca tttttcagac
   115441 atttgccatg cttaaatgtg atgtcatcac gtattagcat ggcctttccc gttatactgc
   115501 cagcgtaaag gataagtcac atatttctgg aggggatatg attcttgagc gcgttgaaat
   115561 tgtgggtttt cgcggtatca accgtttgtc gttgatgctg gaacaaaaca acgtcctgat
   115621 tggggagaac gcgtggggta aatccagctt gctggacgcc ttaaccctgc tgctatcgcc
   115681 agaatcagat ctctaccatt ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa
   115741 cgggcgagaa catcatctgc atattatttt gaccttccgc gaatcgctgc caggccgaca
   115801 tcgggttcgc cgctatcggc cgctggaagc gtgctggacg ccatgcaccg atggttatca
   115861 ccgtattttt tatcgtctgg aaggggagag tgcggaagac ggcagcgtga tgacactccg
   115921 cagttttctc gataaagacg gacatccgat tgatgttgag gatattaacg atcaggctcg
   115981 tcatctggtg cgtttaatgc cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat
   116041 tcgtaacggc acggtgccaa atgtccccaa tgtggaagtc accgcgcgcc agctcgattt
   116101 cctcgcccgt gaattatcct cacatccgca aaatctctct gatgggcaga ttcgtcaggg
   116161 actttccgca atggtgcaac tgcttgagca ttatttttct gagcagggag ctggacaggc
   116221 gcgatatcgt ttaatgcggc ggcgagccag caatgagcaa cgaagctggc gctatctgga
   116281 tatcatcaac cgaatgattg accgacctgg agggcgctcg tatcgggtta ttttgctcgg
   116341 cctgtttgct actttattgc aggcaaaagg cacattgcga ctggataaag acgcccgtcc
   116401 attgttgctg atcgaagatc cagaaacccg tttacacccc attatgcttt cagttgcctg
   116461 gcatctgttg aaccttctgc cattgcagcg catcgctact accaactcgg gagagttgct
   116521 ttcgttaacg ccggtagagc atgtttgccg actggtacga gagtcctctc gcgttgccgc
   116581 ctggcgtctg ggaccgagtg gcttgagtac cgaagatagc cgacgcatct cctttcacat
   116641 tcgttttaac cgtccgtcat cgctgtttgc ccgctgttgg ctgctggtgg aaggggaaac
   116701 ggaaacctgg gttatcaatg aactggcgcg tcagtgcgga catcattttg atgccgaagg
   116761 gatcaaggtc attgagtttg cccagtccgg actaaagcca ctggttaaat ttgcccggcg
   116821 aatggggatt gaatggcatg tactggtcga tggcgatgaa gcagggaaga aatatgccgc
   116881 tacggtacgc agcctgttga ataacgatcg ggaagccgaa cgagaacatt taacggcgtt
   116941 accggcgctg gatatggaac attttatgta tcgccaggga ttttccgatg tgttccaccg
   117001 cgtggcgcaa atcccggaaa atataccgat gaatctgcgc aaagttatct cgaaagcgat
   117061 ccatcgctct tccaaacccg atcttgccat tgaagtggca atggaggccg gacgtcgtgg
   117121 tgtggactcc gtaccgacgc tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg
   117181 cggtcgggcc gactaactac aaaacatcgt ggccatttgt ggctgaatag cgtcgagcat
   117241 ctcatagcgc cgacggtatt cagcccgttt tttactggcg atttcggcaa tctcttttcg
   117301 tgctatctgt gctggaaggc ggtaatggcg ttcagcatca catacgccgc caaccgattc
   117361 ccagaaagcg ttgtaatcgg catggatctt gccctcttta tcgcgataac gcaggctgcg
   117421 gtaaatatgc gtttcattgc tgacggcaat aatctgctct acctgcaaac gttgggcaaa
   117481 cagacaggcc gcttccatca cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc
   117541 attctggatt tcctggtgtg gaatttccca ttttgcgcct tgcagtccgc caataaacat
   117601 cgtccttttc ccctgatatt cacacagggt aaaagtgagc tctgccagag gaataccttc
   117661 gctgttgcgg aacaggatgg tgctgtcacc ttctttatcc attgagatca tcatggtcag
   117721 ctcaagcgtg aactgctcgc cgtttttgcc ttccagcttc gccagttgca gcccgggggt
   117781 attcaaatat aagctgaatt cttccgccga catacacccg cggagtaacg cataatggta
   117841 acgtaacgcc tccagcaatt gcttacggct aagattcgcc gcaaggtaag ggcgatgtag
   117901 acgtacaggc agtcgcggct ggcgcgttaa caacacattg agattaggcc agtgggaaag
   117961 ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac gagcgcagca aaaatttctg
   118021 gcgaaaacta cggcgatgcc agaatttacc cggccgacac tgtcctcgtg ccagactaag
   118081 aaaaagtgac aggctgctga gagattcaga tggcgtaaag gtccgttcag ttagctgcga
   118141 catattcatg aaatcaatgg ttatacatga cgtcgattta accattgcgt atcttaacca
   118201 aacatcaata gtgtgattat taacgtaaat tttagggttt tgttgatatt tcgttgaagt
   118261 taatgacccg gattggcata tggagtattc agaatattta tgaaaaagcg gaaaaccgtg
   118321 aagaagcgtt acgttattgc gatggtgata gtcatcgccg ggctgattac gttatggaga
   118381 attcttaacg cacccgtgcc gacttatcag acgctgattg tgcgccccgg tgatttacag
   118441 caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta aggttgacgt gggcgcgcag
   118501 gtcagcggtc agttgaaaac gctgtcggtg gcgattggcg ataaagtaaa aaaagaccag
   118561 cttttagggg ttattgatcc tgaacaggct gaaaaccaga tcaaggaggt ggaagcaacg
   118621 ctgatggagc tacgcgcgca gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg
   118681 acgtattccc gtcagcaacg tcttgcacaa acgcaagcgg tttcacagca ggatctcgat
   118741 aacgccgcga cggagatggc tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc
   118801 aagcgcaatc aggcctctct cgatacggct aaaaccaatc tcgattacac ccgcatcgtt
   118861 gccccgatgg ccggggaagt cacgcaaatc accactctgc aaggccagac ggtgattgcc
   118921 gcacaacaag caccgaacat tctgacgctg gcagatatga gcaccatgct ggtaaaagcg
   118981 caggtttctg aagcggatgt aatccacctg aagccggggc aaaaagcctg gtttacggtg
   119041 cttggcgatc cactgacgcg ctacgagggg caaatcaagg atgtgctacc gacgccggaa
   119101 aagattaacg acgctatttt ctattacgcc cgttttgaag tacccaatcc caatggttta
   119161 ctgcggctgg atatgaccgc gcaagtgcat attcagctca ccgatgtgaa aaatgtgctg
   119221 acgatccctc tgtcggcgtt aggcgatccg gttggcgata atcgttataa agtcaaattg
   119281 ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat
   119341 gttgagattg tcaaagggct ggaagcgggc gatgaagtgg tgattggtga ggccaaacca
   119401 ggagctgcgc aatgacaccc ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg
   119461 gtgatgagca ggttgaggtg ctgaagggca tcagcctcga tatttatgct ggtgagatgg
   119521 tggcgattgt cggcgcttcg ggttccggta aatcgaccct gatgaatatt ctcggctgcc
   119581 tggataaggc caccagcggc acctatcgcg tagccggtaa ggatgttgcc acgctggacg
   119641 ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt tattttccag cgttaccatt
   119701 tgctttcgca tttaaccgcc gagcagaacg ttgaagtacc cgccgtctat gccggtcttg
   119761 agcggaaaca gcgactgctt cgcgcccagg agttactgca acgactgggg ctggaagacc
   119821 gtacagagta ttacccggca cagctttcgg gtggccagca acagcgcgtc agtatcgcgc
   119881 gggcattgat gaacggcggt caggtaattc ttgccgatga accgaccggc gcactggaca
   119941 gccattctgg cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg
   120001 tgattatcgt cacccacgat ccgcaggttg ctgctcaggc cgagcgggtg atcgaaattc
   120061 gcgacggcga aattgtgcgc aatcctcccg ccgttgaaaa agtgaatgct actggcggaa
   120121 cggaacctgt tgtcaacacg gcgtctggct ggcggcagtt tgtcagcggt tttaacgagg
   120181 cgctgacgat ggcatggcgg gcgctggcag cgaataaaat gcgtacttta ctgaccatgc
   120241 tggggattat tatcggtatt gcgtcggtgg tttccattgt cgtggtgggc gacgccgcca
   120301 aacaaatggt gctggcggat attcgttcta ttggtacgaa tactattgat gtctatcccg
   120361 ggaaagattt tggcgatgac gatccgcaat atcagcaggc gctgaagtac gacgacttaa
   120421 tcgccatcca aaaacaaccg tgggttgcct ctgccacgcc cgccgtctcg caaaacctgc
   120481 gcctgcgtta taacaatgtt gatgtggctg ccagtgccaa tggtgtgagc ggcgattatt
   120541 ttaatgtcta tggcatgacc ttcagtgaag ggaacacctt taatcaggag cagctgaacg
   120601 gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg ccagcttttc ccccataaag
   120661 cagatgtggt tggcgaggtg attctggtcg gcaatatgcc cgccagagtc attggtgtgg
   120721 cggaagaaaa acagtcgatg tttggtagca gtaaagtgct gcgtgtctgg ctgccataca
   120781 gcacgatgtc cgggcgagtt atggggcagt cgtggctgaa ctccattact gtcagggtga
   120841 aagaaggctt tgacagcgct gaggcggaac agcaactcac gcgtttactt tcactgcgcc
   120901 acggtaagaa ggatttcttt acctggaaca tggatggcgt cttgaaaacc gttgaaaaga
   120961 ccacacgtac tttacaactg tttctgacgc tggttgcggt gatttcgctg gtggtgggcg
   121021 gtattggtgt aatgaatatt atgctggtgt cagtgaccga gcggacgcgg gaaattggca
   121081 ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca acagtttttg atcgaagccg
   121141 tactggtttg cctggttggt ggcgcgttgg gaataacact gtcactgtta attgctttca
   121201 ccttgcagct tttcttaccc ggctgggaga ttggtttttc accgttagcg ctgctgctgg
   121261 cgtttctctg ctcgacggtc accgggattt tatttggctg gttacccgca cgaaatgcgg
   121321 cacgactgga tccagtagat gctctggcac gagagtaatt tttgagataa aaatgccagc
   121381 cgatcgggct ggcattttgc ctttaggatg tacacaatga gacagaagag ctatgcgact
   121441 gccgcttcta cttcgacggg cacaataaca ctggcgtgat tgccttttgg cccctggtgg
   121501 acatcaaact gaacggattg tccagctttt agcgttctgt aaccatccat ctgaatggta
   121561 gaataatgag cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc aaaccctttg
   121621 gcattgttga accacttaac agtacccttt tccatgcttc gacatccttc gcaaatctta
   121681 tacaagtaag atggaataaa ccggggtcag agagggggct gttcaaaacc tcgccaactc
   121741 tagaaataca atttagagaa ttagggcgag ccgtcaagca tttgacaggg gccagggggc
   121801 aggtatgaat caaaaatttg aagcagttaa cgctattgac aggaatgtga cagatgtcgc
   121861 tgatgctaac gatagatgat agttatctat catgtggagt aaattggtca ggcaaataag
   121921 ctcttgtcag cggcagggcg ttctgccgat aaccgtaacc gaagatgata actgacaatg
   121981 ggtaaaacga acgactggct ggactttgat caactggcgg aagaaaaagt tcgcgacgcg
   122041 ctaaaaccgc catctatgta taaagtgata ttagtcaatg atgattatac tccgatggag
   122101 tttgttattg acgtgttaca aaaattcttt tcttatgatg tagaacgtgc aacgcaattg
   122161 atgctcgctg ttcactacca ggggaaggcc atttgcggag tctttaccgc tgaggttgca
   122221 gaaaccaaag tggcgatggt gaacaagtac gcgagggaga atgagcatcc attgctgtgt
   122281 acgctagaaa aagcctgaat gcaggcataa aaattggggg aggtgcctat gctcaatcaa
   122341 gaactggaac tcagtttaaa tatggctttc gccagagcgc gcgagcaccg tcatgagttt
   122401 atgaccgtcg agcacttgtt actggcgctg ctcagtaacc catctgcccg ggaggcgctg
   122461 gaagcgtgtt ctgtggattt ggttgcgctc cgtcaggaac tggaagcctt tattgaacaa
   122521 accacacccg ttctgcctgc cagtgaagag gagcgcgaca cacagccgac gctgagtttt
   122581 cagcgtgtac tgcaacgtgc ggtcttccat gtccagtcct ccggtcgcaa tgaggttacc
   122641 ggtgcaaacg ttctggtcgc tatctttagc gaacaggagt cgcaggcggc atatctgttg
   122701 cgcaaacatg aagtcagccg tctcgatgtg gtgaacttta tctctcatgg cacgcgtaaa
   122761 gacgagccga cacagtcttc tgatcctggc agccagccaa acagcgaaga acaagctggt
   122821 ggggaggaac gtatggagaa tttcacgacg aacctgaatc agcttgcgcg agtgggcgga
   122881 atcgacccac tgattggtcg tgagaaggag ctggagcgtg ctattcaggt tctctgccgt
   122941 cgccgtaaaa acaacccgct gctggtgggg gagtctggtg tcggtaaaac cgcgattgcg
   123001 gaggggcttg catggcggat tgttcagggc gatgtgccgg aagtgatggc tgactgtacg
   123061 atttactctc tcgatatcgg ttctctgtta gcgggcacaa aatatcgcgg cgactttgaa
   123121 aaacgtttta aagcgttgct caagcagctg gagcaagaca ctaacagcat cctgtttatt
   123181 gatgagatcc acaccattat cggtgcgggt gcagcgtctg gtggccaggt cgatgcggct
   123241 aacctgatta aaccgttgct ctccagcggt aaaatccgcg taattggttc gacaacctat
   123301 caggagttca gcaacatttt cgagaaagac cgtgctctgg cgcgtcgctt ccagaaaatt
   123361 gatattactg aaccgtcaat cgaagaaact gttcaaatca tcaatggcct gaaaccgaag
   123421 tatgaagcgc accacgacgt gcgttatacc gcaaaagcgg tgcgtgctgc ggtagagctg
   123481 gcggtgaaat acattaacga tcgtcatctg ccggataaag cgattgatgt tatcgacgaa
   123541 gcgggcgctc gcgcacgtct gatgccggta agcaaacgca agaaaaccgt taatgtggcg
   123601 gatattgaat ccgtggtggc ccgtattgca cgcattccag agaagagtgt ttctcagagt
   123661 gaccgcgata ccctgaaaaa cctcggcgat cgcctgaaaa tgctggtctt cggtcaggat
   123721 aaagccattg aggcgctgac tgaagccatt aagatggcgc gtgcaggttt aggtcacgaa
   123781 cataaaccgg tcggttcgtt cctgtttgcc ggccctactg gggtcgggaa aacagaggtg
   123841 acggtacagc tttcgaaagc gttgggcatt gagcttctgc gctttgatat gtctgagtat
   123901 atggaacgcc ataccgtcag ccgtctgatt ggtgcgcctc cgggatacgt tggttttgat
   123961 cagggcggtt tgctgactga tgcggtcatc aagcatccac atgcggtgct gctgctggac
   124021 gaaatcgaga aagcgcaccc ggacgtgttc aacatcctct tgcaggtgat ggataacggt
   124081 acgctgaccg ataacaacgg acgcaaagcg gacttccgta acgtggtgct ggtgatgacc
   124141 actaacgcgg gggtacgtga aaccgagcgc aaatccattg gtcttatcca ccaggataac
   124201 agcaccgatg cgatggaaga gatcaagaag atctttacac cggaattccg taaccgtctc
   124261 gacaacatta tctggttcga tcatctgtca accgacgtga tccatcaggt ggtggataaa
   124321 ttcatcgtcg agttgcaggt tcagttggat cagaaaggtg tttctctgga agtgagccag
   124381 gaagcgcgta actggctggc cgagaaaggt tacgaccggg caatgggcgc acgtccgatg
   124441 gcgcgtgtca tccaggacaa cctgaaaaaa ccgctcgcca acgaactgct gtttggttcg
   124501 ctggtggacg gcggtcaggt caccgtcgcg ctggataaag agaaaaatga gctgacttac
   124561 ggattccaga gtgcacaaaa gcacaaggcg gaagcagcgc attaatctga ttgtcaggta
   124621 ggttggttaa gtccgtgatc tcgtcagggg ttacggactt tttatttatg gggggaggag
   124681 gttcagaccc tttttttaat gatgatgcta agttattgat aattagtgct gcgggtaggt
   124741 aaggataaaa aagggtggca gcaggagatt gagatggttt tgctttatta acaacgggct
   124801 aaacgtgtag tatttgagtt cactgccgta caggcagctt agaaaggaat atgtcgccat
   124861 cgaacgtgcg aaaccgcgtt cactgccgta caggcagctt agaaatgacg ccatatgcag
   124921 atcattgagg cgaaaccgtt cactgccgta caggcagctt agaaaggaag agacggatgt
   124981 tgaccagcga aatccgagtt cactgccgta caggcagctt agaaaatagt ttgtgcgtct
   125041 tttgagtcag tccacgcgtt cactgccgta caggcagctt agaaaaggga ttgatggcgg
   125101 aagtcagagc acaaaaggtt cactgccgta caggcagctt agaaacgtcg agaccaatcg
   125161 actggtggtg aactgtgcgt tcactgccgt acaggcagct tagaaaagca gcggtggcga
   125221 accgcgcgtt gttgatttgt tcactgccgt acaggcagct tagaaaagaa ctcgaaattt
   125281 atcgcgggac ttttcagcgt tcactgccgt acaggcagct tagaaaaggc tgctggcgct
   125341 ctcgttgtta acggcagcgt tcactgccgt acaggcagct tagaaagttc cacgcggata
   125401 tctgtaattt ccagcaaagt tcactgccgt acaggcagct tagaaagctc gcgtcatgga
   125461 atgggcgaaa ttgcgcaagt tcactgccgt acaggcagct tagaaatgga ggaagacgtc
   125521 tgatgccgaa atatttaagt tcactgccgt acaggcagct tagaaagtgc ggacgtgatg
   125581 aatctccgct gggctttcgt tcactgccgt acaggcagct tagaaaacgt tcgcaccggt
   125641 cagggtactg cgcagcgtgt tcactgccgt acaggcagct tagaaagaaa ccagagcgcc
   125701 cgcataaaac aggcacaagt tcactgccgt acaggcagct tagaaagcca gcataaaacc
   125761 gcctttgata ttttattggt tcactgccgt acaggcagct tagaaatcag ccggaggctc
   125821 tcaatttcag ccgcgcgggt tcactgccgt acaggcagct tagaaaagca cggctgcggg
   125881 gaatggctca atctctgcgt tcactgccgt acaggcagct tagaaatgat ggcgcagcag
   125941 tcctccctcc tgccgccagt tcactgccgt acaggcagct tagaaactga acgttgaaga
   126001 gtgcgaccgt ctctccttgt tcactgccgt acaggcagta ttcacactac gatagtgtta
   126061 gacgtttggt cgtgcaatga cactctcaac ttcaaaccat tagcgttagc acgcaataac
   126121 aatcgtaata attgcgatgg aaatcaattt tcagcacata aatcaatgct gtactaagcc
   126181 caataccttc aaatataaaa taatcacagg atgtgtttat gtcttcgaat taccttacgc
   126241 cttccgatct caaaaccatt ctccactcca aacgcgccaa tatttattat ctggaaaaat
   126301 gccgggtaca ggtgaatggt gggcgggtgg agtatgttac cagcgaaggt aaagagtcgt
   126361 actactggaa tatccccatt gcgaatacca cggcgttgat gctgggaatg ggaacttccg
   126421 ttacccaggc ggcgatgcgt gaatttgctc atgccggggt gatggtaggc ttttgtggta
   126481 cggatggcac gccgctgtat tcagcaaatg aagtggatgt tgatgtctcc tggctcagcc
   126541 cacaaagtga atatcgccct acagagtatc tccagcagtg ggtttctttc tggtttgttg
   126601 aagacaaacg acttgctgcc gcgaaacgtt ttcagttaat ccgcctgaca catatcgata
   126661 agcactggtc cagctcgaaa atgctgcgtg aacacgcatt tcaacctgat gttaatgcgc
   126721 tgcataccct cctgaatcgt acttgtgaag aaatagacgc ggcagaaaac catacgcagc
   126781 ttatgctcgt tgaggccaaa ttgactaaag cgttatacaa aatggtcagt caaaccgtgg
   126841 ggtatggcga tttcactcgc gcaaaacggg gcggtggtat cgatatggcc aaccgcttcc
   126901 tcgatcaagg gaattatctg gcttatggac tggcagcagt ggcggcgtgg gtaacaggta
   126961 tcccgcacgg tttagctgta atgcatggta agacacgtcg cggcgggctt gtttttgacc
   127021 ttgccgatct gattaaagat gctcttgtta tgccgcaagc atttattgcc gcaatggcgg
   127081 gagaagatgc tcaggagttt cgtcaacgct gtgttaatat ctttcaacaa gcggatgcgc
   127141 tggatgtcat gatcacctct ctgcaggaaa cggcccaggc actggctaag gctgaccaat
   127201 gaatgtccta attatctcac gctgtacgaa aaatgctcgc gtggaaagct gccgtattat
   127261 tgatcagttt gccgaacgca ctggtgacgc agcatggcaa acggtgatta ctctggaagg
   127321 cgttaataca ctccgccgtt tactgcgcaa aaccgccagg cgaaataccg cagtggcttg
   127381 tcactggctg aagaataatg ggcaaacgga gctgatgtgg attgtcggca atattcgtcg
   127441 ctttaatgct caaggccgcg ttcccacaaa tcgaacaacg caaatcagcc tggtgaacaa
   127501 agaagaaaat cgctggcagt gcgcggaaag tattgcactg ctggcggcta tcgctgggct
   127561 atttcatgat tttggtaaat caggacgcag ctttcagcaa agcctgactc aaaagaatac
   127621 ccgcagttat caaccttacc gacatgaatg gatctcactg cggctctttc agacgtttgt
   127681 cggtgatcgg cccgacgatg tgtggctggc gaagcttggc gaactcacgg cagatgatga
   127741 atctgcgatt cttgagcgcc tgcaaaaaga cactcctcaa ttttcaaata gcccgttcag
   127801 ttcgctgcaa ccattagcca aaacggtagg ctggctgatc ctttcccatc atcgaatgcc
   127861 ggagaatctt tctccacagt cggaacaatc atgtaaaggg tgtgaaagtt ggctggagaa
   127921 gcaacttaat gctgactgga atgccgttaa ccaccgcaga gatgactggt cacaacagga
   127981 ttttgcgcac gtctggcagt ttccatcagg aacgccgctt caaagcgcga gctggcggga
   128041 aaaagcgcgt cagatagccc gccgggcaag caacgcgctc ggtttaaaga catatgggca
   128101 gatggaacag ttgttcccct tacatatggc gcgtttttgt ctgatgcttg ccgatcatta
   128161 ctattcctca agcgatgcgc agcaaaaatg gcaggacccg gattatatcg tctgggcaaa
   128221 tagcgatcgc caaagcggca cgctcaaaca acgggtagac gaacaccttg tgggcgtcgc
   128281 gcatcatgcg tatctgcttg ggcggcaact gatgcacaca cgggcatcgc ttccggcaat
   128341 tgcccgacat aaaggtttta gccagcgggc tacagatgtg aattatcgct ggcaagataa
   128401 agcctgggat gtggcgcgag cgttacgtga acgaagtcgt gaagagggat ttttcggcgt
   128461 caatatggcc tcgacggggt gtggtaaaac ctttgcgaac gcccgcatta tgtatgcact
   128521 tgccgacgag cgagaagggt gccgctttac cgttgcgctg ggattgcgca cgctgacttt
   128581 acaaaccggc gatgctctgc gcagtcggct tgggctgggt gaggatgagc tggcggtact
   128641 gattggttcg gcggcagtta atcaactgtg gcaacaggaa aaaatagaca atgattgcgg
   128701 aagtgcttcg caagagtcgc cggcagagga gcaacagttc gttaagtacg aaggcagcct
   128761 gcataccggg gcgctggaaa aatggttgaa ggatgacagc aaacttaagg agttagtaag
   128821 cgcgccgatt ctggtctcga cgattgatca tcttatttcc gcgaccgagg gcgtgcgagg
   128881 cgggaaacag cttccggcga tgttgcgcct gttaaccagc gatttagtgc tcgacgaacc
   128941 tgatgacttt gatattgccg atcttcatgc ggtatgtcga ttgatgaact gggccgggat
   129001 gctgggaacg cgggtattac tttcttccgc gacgttaccg ccaggtttga ttcaggcgct
   129061 gttcgcggct tatcttgctg ggcgaaaaat gtggcaggcg tcttgtggta tcaacggcag
   129121 gcctgtcaat atatgctgcg cctggtttga tgaaaaagat gctgatgcca cgcaaattta
   129181 cgacggacct gggtttcgtg atgctcatgc caaatttgtt gcacgacgag cggtaatgct
   129241 ggctgaaaaa gaacgactgc atttcgggcg tgtagcttcg gtttcatcag catcaggcgc
   129301 gatacaagat gttaccgaaa gtgtggccca gacggtgcat acgcaaatgc tgaagttgca
   129361 tcaggcgcat cgccagcgac acgaaagcgg taaaaccgtt tcgctgggcc tggtgcgctt
   129421 cgcgaatatc aatccactgg ttgcggtgac caaagccctg attgccattc cttcaccaga
   129481 agatgtctgc attcattact gtgtttacca ctcccgacat ccgctggcgg tgcgttcaga
   129541 tattgagaag cgactggacc gcgcttttac tcgtcataac gaactcgatt tctggaacaa
   129601 cgaagatatc gcagatgcct tacataaccg tccggaatcg caccatctgt ttgtggtgtt
   129661 ggggacatcg gttatagaag tggggcgtga ctgggatgcg gactggggaa ttatcgaacc
   129721 aagctccatg cgttcgataa ttcaatttgc cggacgtatt cagcgccacc gtagaaatgt
   129781 gccgaccagt gagaatctgg ttattttgcg tagcaatatc aaatcattgc agaaaaaagc
   129841 ccccgcattt tgtaagcctg gtttcgaaac aaaagaacac ttgctgaata gccacgatat
   129901 ttgcgaactg attccgatcg cggactaccg gacgattaac gccttgccgc ggatcctgtc
   129961 tgccaataat gccaacaaac tggcggagct agaacatagt cgtttatccg ccgaattgat
   130021 gagcagtaaa agcaaacatg tggtggcggc gcaatggtgg cgtgcaccgc taagctggaa
   130081 cggctggatg cagcgccaga cgccatttcg ttattcccct ttgccggagg cggtgttttt
   130141 tctgcatatg gatgatgaag aaagcgaagc gcgtttctat tcccgcacgg gcgacaacga
   130201 gcgtaaagcg caagggaatt tcaggcgaga agaggtgaga tgcgccagcg gcgtcgcttg
   130261 ctggggcgtg atggattacc aacacgtttt gttgaattta gctgataagc aaagttgtga
   130321 gatagcaaac gttggggaaa agtttgcgga ggtgagagta cccgtcagtg aagaagatgc
   130381 ggtcgatgac tggcgttatc acccctggtt aggtgtgttt cgcaacatgt gatggggcgc
   130441 gagctgcctg tacggcagtt tacaagtccg tatccggaga tacctctatt tctaagctgc
   130501 tcaaggcagt ttgggtatgt tagtgcttca actattgcgt ggcaaatatt aagttgcttt
   130561 aatatcacct atggtgatta catgagattt aataatatct tttagaagat atctctagga
   130621 gagtttgttt gtgtgatcac cactggtgtt gtcccttttg ctgtgtatgt cagttgtact
   130681 gaagccatgt tgggctacgg agatttttca gtgaaatcag ggagcaactt cgacttgatg
   130741 gattaacgaa aaggagccga tatggaaaaa acggcgctaa tacaatttat tactgaatac
   130801 atcgcttcgc gaaggcaacc caaaatcgat gcctttgaaa aagaagccgc aaagcgagtg
   130861 gaacagggcg aggacgccag cgttatcgct caggagcggc aggaactcga agcacgctac
   130921 ttgccacgta actggttaac ggatgcggca aagcgtgccg ggcaaatcaa attagtcact
   130981 catggcgcta aattcagtca tggtgattct aaagccagta gcttttatct ggaaacctcc
   131041 gctaacgaaa gctatttaaa cacagcatcg ttggcgaatg tcgctactga cgcgattggt
   131101 aacgccgctg cacttgacgt agcaaagttg ctacaaaccg atgtgaacgg agattcactg
   131161 cttgccagcc tgaagcgtgg tgattatcag gcattaagta cgtttgcaga ggataaagtg
   131221 caactggagc tgtgggtagc tgggtttagc caggcatgga ctactggaca gccttccagc
   131281 cataaactgg cgaaacaaat ctatttccct gttgcggatg gttatcacct gttatgccca
   131341 ctgttttcca gttcactggc ccaggcgatg tatgaaaagc taaccgccgg gcgctttagt
   131401 gaggaatcga aagccattcg cgatgcccgc aaagcaggaa
//
LOCUS       DADTFG010000002        79513 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.002, whole genome shotgun sequence.
ACCESSION   DADTFG010000002 DADTFG010000000
VERSION     DADTFG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.002"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(215..1615)
                     /gene="asnS"
                     /locus_tag="J1138_000135"
     CDS             complement(215..1615)
                     /gene="asnS"
                     /locus_tag="J1138_000135"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBA8451989.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1784..2986)
                     /gene="pncB"
                     /locus_tag="J1138_000136"
     CDS             complement(1784..2986)
                     /gene="pncB"
                     /locus_tag="J1138_000136"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBA8451990.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3252..5864
                     /gene="pepN"
                     /locus_tag="J1138_000137"
     CDS             3252..5864
                     /gene="pepN"
                     /locus_tag="J1138_000137"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBA8451991.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAVELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRAFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(5907..6674)
                     /gene="ssuB"
                     /locus_tag="J1138_000138"
     CDS             complement(5907..6674)
                     /gene="ssuB"
                     /locus_tag="J1138_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA8451992.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWLKHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGEFETRLRKQG"
     gene            complement(6671..7462)
                     /gene="ssuC"
                     /locus_tag="J1138_000139"
     CDS             complement(6671..7462)
                     /gene="ssuC"
                     /locus_tag="J1138_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBA8451993.1"
                     /translation="MATPVKKWLLRVAPGFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7474..8619)
                     /gene="ssuD"
                     /locus_tag="J1138_000140"
     CDS             complement(7474..8619)
                     /gene="ssuD"
                     /locus_tag="J1138_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBA8451994.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFALTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8616..9575)
                     /locus_tag="J1138_000141"
     CDS             complement(8616..9575)
                     /locus_tag="J1138_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8451995.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9568..10143)
                     /gene="ssuE"
                     /locus_tag="J1138_000142"
     CDS             complement(9568..10143)
                     /gene="ssuE"
                     /locus_tag="J1138_000142"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBA8451996.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHNPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10380..11390
                     /gene="pyrD"
                     /locus_tag="J1138_000143"
     CDS             10380..11390
                     /gene="pyrD"
                     /locus_tag="J1138_000143"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBA8451997.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11564..12106
                     /gene="zapC"
                     /locus_tag="J1138_000144"
     CDS             11564..12106
                     /gene="zapC"
                     /locus_tag="J1138_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBA8451998.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(12103..13212)
                     /gene="ycbX"
                     /locus_tag="J1138_000145"
     CDS             complement(12103..13212)
                     /gene="ycbX"
                     /locus_tag="J1138_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBA8451999.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANTTQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAIGDDGTILCCSCVPKTALKLAR"
     gene            13310..15418
                     /gene="rlmKL"
                     /locus_tag="J1138_000146"
     CDS             13310..15418
                     /gene="rlmKL"
                     /locus_tag="J1138_000146"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBA8452000.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWTQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVNDVAQLANPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15430..17337
                     /locus_tag="J1138_000147"
     CDS             15430..17337
                     /locus_tag="J1138_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8452001.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17467..18720
                     /gene="pqiA"
                     /locus_tag="J1138_000148"
     CDS             17467..18720
                     /gene="pqiA"
                     /locus_tag="J1138_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBA8452002.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            18725..20365
                     /gene="pqiB"
                     /locus_tag="J1138_000149"
     CDS             18725..20365
                     /gene="pqiB"
                     /locus_tag="J1138_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBA8452003.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20362..20925
                     /gene="pqiC"
                     /locus_tag="J1138_000150"
     CDS             20362..20925
                     /gene="pqiC"
                     /locus_tag="J1138_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBA8452004.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21181..21348
                     /gene="rmf"
                     /locus_tag="J1138_000151"
     CDS             21181..21348
                     /gene="rmf"
                     /locus_tag="J1138_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBA8452005.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21418..21936)
                     /gene="fabA"
                     /locus_tag="J1138_000152"
     CDS             complement(21418..21936)
                     /gene="fabA"
                     /locus_tag="J1138_000152"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBA8452006.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(22005..23765)
                     /locus_tag="J1138_000153"
     CDS             complement(22005..23765)
                     /locus_tag="J1138_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBA8452007.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEDKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            23951..24403
                     /gene="matP"
                     /locus_tag="J1138_000154"
     CDS             23951..24403
                     /gene="matP"
                     /locus_tag="J1138_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBA8452008.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24481..25521)
                     /gene="ompA"
                     /locus_tag="J1138_000155"
     CDS             complement(24481..25521)
                     /gene="ompA"
                     /locus_tag="J1138_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBA8452009.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(25877..26386)
                     /gene="sulA"
                     /locus_tag="J1138_000156"
     CDS             complement(25877..26386)
                     /gene="sulA"
                     /locus_tag="J1138_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBA8452010.1"
                     /translation="MYTAGYAHRDSSFSSTASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            26605..27234
                     /gene="sxy"
                     /locus_tag="J1138_000157"
     CDS             26605..27234
                     /gene="sxy"
                     /locus_tag="J1138_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBA8452011.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(27197..29359)
                     /gene="yccS"
                     /locus_tag="J1138_000158"
     CDS             complement(27197..29359)
                     /gene="yccS"
                     /locus_tag="J1138_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBA8452012.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVLA"
     gene            complement(29369..29815)
                     /locus_tag="J1138_000159"
     CDS             complement(29369..29815)
                     /locus_tag="J1138_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBA8452013.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            29938..31992
                     /gene="helD"
                     /locus_tag="J1138_000160"
     CDS             29938..31992
                     /gene="helD"
                     /locus_tag="J1138_000160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBA8452014.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREVWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMHERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVDILKNLDVPVAR
                     KP"
     gene            complement(32024..32482)
                     /gene="mgsA"
                     /locus_tag="J1138_000161"
     CDS             complement(32024..32482)
                     /gene="mgsA"
                     /locus_tag="J1138_000161"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBA8452015.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(32578..33240)
                     /locus_tag="J1138_000162"
     CDS             complement(32578..33240)
                     /locus_tag="J1138_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBA8452016.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            33413..33826
                     /locus_tag="J1138_000163"
     CDS             33413..33826
                     /locus_tag="J1138_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBA8452017.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(33871..34188)
                     /gene="hspQ"
                     /locus_tag="J1138_000164"
     CDS             complement(33871..34188)
                     /gene="hspQ"
                     /locus_tag="J1138_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBA8452018.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(34246..35436)
                     /gene="rlmI"
                     /locus_tag="J1138_000165"
     CDS             complement(34246..35436)
                     /gene="rlmI"
                     /locus_tag="J1138_000165"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBA8452019.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSHEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            35531..35809
                     /gene="yccX"
                     /locus_tag="J1138_000166"
     CDS             35531..35809
                     /gene="yccX"
                     /locus_tag="J1138_000166"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBA8452020.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGDEGQVEKLIQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(35806..36135)
                     /gene="tusE"
                     /locus_tag="J1138_000167"
     CDS             complement(35806..36135)
                     /gene="tusE"
                     /locus_tag="J1138_000167"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBA8452021.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(36227..36886)
                     /gene="yccA"
                     /locus_tag="J1138_000168"
     CDS             complement(36227..36886)
                     /gene="yccA"
                     /locus_tag="J1138_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBA8452022.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(37093..37180)
                     /locus_tag="J1138_000169"
     tRNA            complement(37093..37180)
                     /locus_tag="J1138_000169"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37144..37146),aa:Ser,seq:tga)
     gene            37607..38725
                     /gene="hyaA"
                     /locus_tag="J1138_000170"
     CDS             37607..38725
                     /gene="hyaA"
                     /locus_tag="J1138_000170"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBA8452023.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            38722..40515
                     /gene="hyaB"
                     /locus_tag="J1138_000171"
     CDS             38722..40515
                     /gene="hyaB"
                     /locus_tag="J1138_000171"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBA8452024.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            40534..41241
                     /gene="hyaC"
                     /locus_tag="J1138_000172"
     CDS             40534..41241
                     /gene="hyaC"
                     /locus_tag="J1138_000172"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBA8452025.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            41238..41825
                     /locus_tag="J1138_000173"
     CDS             41238..41825
                     /locus_tag="J1138_000173"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBA8452026.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            41822..42220
                     /gene="hyaE"
                     /locus_tag="J1138_000174"
     CDS             41822..42220
                     /gene="hyaE"
                     /locus_tag="J1138_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBA8452027.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            42217..43074
                     /locus_tag="J1138_000175"
     CDS             42217..43074
                     /locus_tag="J1138_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBA8452028.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEVQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINMTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            43208..44752
                     /gene="appC"
                     /locus_tag="J1138_000176"
     CDS             43208..44752
                     /gene="appC"
                     /locus_tag="J1138_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBA8452029.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            44764..45900
                     /gene="appB"
                     /locus_tag="J1138_000177"
     CDS             44764..45900
                     /gene="appB"
                     /locus_tag="J1138_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBA8452030.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            45913..46005
                     /gene="cbdX"
                     /locus_tag="J1138_000178"
     CDS             45913..46005
                     /gene="cbdX"
                     /locus_tag="J1138_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBA8452031.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            46085..47395
                     /locus_tag="J1138_000179"
     CDS             46085..47395
                     /locus_tag="J1138_000179"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HBA8452032.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKEGCPQP
                     GQVAVIADVDERTRKTGEAFAAGLAPDCVITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFAGHRQTAFRELERVLNFPQSNLCLNRKKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALAPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(47383..47595)
                     /gene="cspH"
                     /locus_tag="J1138_000180"
     CDS             complement(47383..47595)
                     /gene="cspH"
                     /locus_tag="J1138_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBA8452033.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            47881..48093
                     /gene="cspG"
                     /locus_tag="J1138_000181"
     CDS             47881..48093
                     /gene="cspG"
                     /locus_tag="J1138_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBA8452034.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            48104..48292
                     /locus_tag="J1138_000182"
     CDS             48104..48292
                     /locus_tag="J1138_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA8452035.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            48267..48497
                     /gene="ymcE"
                     /locus_tag="J1138_000183"
     CDS             48267..48497
                     /gene="ymcE"
                     /locus_tag="J1138_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBA8452036.1"
                     /translation="MRRWISQNNIWLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            48487..48660
                     /locus_tag="J1138_000184"
     CDS             48487..48660
                     /locus_tag="J1138_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBA8452037.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNSGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(48709..49782)
                     /locus_tag="J1138_000185"
     CDS             complement(48709..49782)
                     /locus_tag="J1138_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBA8452038.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFITRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHVWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(49865..52597)
                     /gene="torS"
                     /locus_tag="J1138_000186"
     CDS             complement(49865..52597)
                     /gene="torS"
                     /locus_tag="J1138_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBA8452039.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGLVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVKNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLDEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
     gene            52680..53708
                     /gene="torT"
                     /locus_tag="J1138_000187"
     CDS             52680..53708
                     /gene="torT"
                     /locus_tag="J1138_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBA8452040.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(53681..54373)
                     /gene="torR"
                     /locus_tag="J1138_000188"
     CDS             complement(53681..54373)
                     /gene="torR"
                     /locus_tag="J1138_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBA8452041.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            54503..55675
                     /gene="torC"
                     /locus_tag="J1138_000189"
     CDS             54503..55675
                     /gene="torC"
                     /locus_tag="J1138_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBA8452042.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            55675..58221
                     /gene="torA"
                     /locus_tag="J1138_000190"
     CDS             55675..58221
                     /gene="torA"
                     /locus_tag="J1138_000190"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBA8452043.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYIPASQVKS"
     gene            58218..58817
                     /gene="torD"
                     /locus_tag="J1138_000191"
     CDS             58218..58817
                     /gene="torD"
                     /locus_tag="J1138_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBA8452044.1"
                     /translation="MTTLTVQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
     gene            complement(59072..59377)
                     /gene="cbpM"
                     /locus_tag="J1138_000192"
     CDS             complement(59072..59377)
                     /gene="cbpM"
                     /locus_tag="J1138_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBA8452045.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(59377..60297)
                     /gene="cbpA"
                     /locus_tag="J1138_000193"
     CDS             complement(59377..60297)
                     /gene="cbpA"
                     /locus_tag="J1138_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBA8452046.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            60832..62073
                     /gene="agp"
                     /locus_tag="J1138_000194"
     CDS             60832..62073
                     /gene="agp"
                     /locus_tag="J1138_000194"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBA8452047.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIHVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(62111..62338)
                     /locus_tag="J1138_000195"
     CDS             complement(62111..62338)
                     /locus_tag="J1138_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452048.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(62359..62955)
                     /gene="wrbA"
                     /locus_tag="J1138_000196"
     CDS             complement(62359..62955)
                     /gene="wrbA"
                     /locus_tag="J1138_000196"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBA8452049.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            63328..63501
                     /locus_tag="J1138_000197"
     CDS             63328..63501
                     /locus_tag="J1138_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBA8452050.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(63584..64806)
                     /locus_tag="J1138_000198"
                     /pseudo
     CDS             complement(63584..64806)
                     /locus_tag="J1138_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
     gene            complement(64827..65321)
                     /gene="rutF"
                     /locus_tag="J1138_000199"
     CDS             complement(64827..65321)
                     /gene="rutF"
                     /locus_tag="J1138_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBA8452051.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMELRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(65332..65922)
                     /locus_tag="J1138_000200"
     CDS             complement(65332..65922)
                     /locus_tag="J1138_000200"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBA8452052.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDALFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(65932..66732)
                     /gene="rutD"
                     /locus_tag="J1138_000201"
     CDS             complement(65932..66732)
                     /gene="rutD"
                     /locus_tag="J1138_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBA8452053.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(66740..67126)
                     /gene="rutC"
                     /locus_tag="J1138_000202"
     CDS             complement(66740..67126)
                     /gene="rutC"
                     /locus_tag="J1138_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBA8452054.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(67138..67830)
                     /gene="rutB"
                     /locus_tag="J1138_000203"
     CDS             complement(67138..67830)
                     /gene="rutB"
                     /locus_tag="J1138_000203"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBA8452055.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARTAGMLIIWFQNGWDEQYVEAGGPGSPNYHKSNALKTMRNQP
                     LLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(67830..68921)
                     /gene="rutA"
                     /locus_tag="J1138_000204"
                     /pseudo
     CDS             complement(67830..68921)
                     /gene="rutA"
                     /locus_tag="J1138_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
     gene            69209..69847
                     /gene="rutR"
                     /locus_tag="J1138_000205"
     CDS             69209..69847
                     /gene="rutR"
                     /locus_tag="J1138_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBA8452056.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(69887..73849)
                     /gene="putA"
                     /locus_tag="J1138_000206"
     CDS             complement(69887..73849)
                     /gene="putA"
                     /locus_tag="J1138_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBA8452057.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAQVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            73904..74113
                     /locus_tag="J1138_000207"
     CDS             73904..74113
                     /locus_tag="J1138_000207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452058.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            74272..75780
                     /gene="putP"
                     /locus_tag="J1138_000208"
     CDS             74272..75780
                     /gene="putP"
                     /locus_tag="J1138_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBA8452059.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            76000..76830
                     /locus_tag="J1138_000209"
     CDS             76000..76830
                     /locus_tag="J1138_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBA8452060.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            76888..78015
                     /locus_tag="J1138_000210"
     CDS             76888..78015
                     /locus_tag="J1138_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBA8452061.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKELFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            78021..79292
                     /gene="efeB"
                     /locus_tag="J1138_000211"
     CDS             78021..79292
                     /gene="efeB"
                     /locus_tag="J1138_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBA8452062.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
BASE COUNT        19362 a        20190 c        21213 g        18748 t
ORIGIN      
        1 tcagacatcc agaatgccaa aaaattgtat gaaaattgtg ttatcagcta agaaaaaact
       61 aatccgcaat aagaatgcag attatttttt gtgcgtaaat gttgcagcgt aatattccgt
      121 aacaggatga tcgttatggt ggggttgcaa acagataact tgacagaaag gataaaaaaa
      181 gagccagcgg gcgctggctc ttgaagacgc taaattagaa gctggcgtta cgcggagtac
      241 gtgggaacgg aataacatca cgtacgtttt gcacgccagt tacgtaagca atcaggcgtt
      301 caaaaccaag accgaaacct gaatgcggaa cagtaccgta gcgacgcaga tcgcgatacc
      361 accagtaatc ttctttgttc aggcccattt caagcatacg ctcgtccagc acgtccagac
      421 gttcttcacg ctgggagcca ccaatgatct caccgatgcc cggagccaga acgtccatag
      481 ccgcaacggt tttaccgtct tcgttaaggc gcatatagaa cgctttaata tcttttggat
      541 agtttttaac cactaccggt gctttaaagt gttcttccgc cagataacgc tcatgctcag
      601 aagagagatc gacgccccag taaaccgggt tttcaaactt cctgccgcag ttttcgagaa
      661 tggtcactgc gtcggtgtaa tccacctgcg cgaaatcggc ctcaatgaag cgttccagac
      721 gtgaaacggc atctttatct acgcgttcag cgaagaattt catgtcgtca gcgcgttctt
      781 cgagaaccgc tttgaagaca tatttcagca tggcttcagc cagacctgcg atatcgttca
      841 ggttagcaaa cgccacttcc ggctccagca tccagaattc cgccaggtga cggctggtgt
      901 tggagttttc agcacggaaa gtcgggccga aggtataaat tttggacaat gcgcaagcgt
      961 aggtttcgcc gttcaactgg ccagatacgg tcaggaaaga ctctttacca aagaagtctt
     1021 tgtcgaaatc cactttgccc tgatcgttac gcggcaggtt ttccagatcc agcgtagaaa
     1081 cacggaacat ttcgcctgca ccttcggtat cagatgcggt aatcagtggc gttgagaccc
     1141 agaagaatcc ctgctcgtta aagaagcgat gcagcgcctg cgccagcgta tggcgaacgc
     1201 gcgcgacggc accaatcagg tttgtgcgcg gacgcaggtg agcgacttca cgcagatact
     1261 caatgctgtg gcgttttgcc gccatcgggt aagtgtctgg atcttcaacc caaccagcaa
     1321 cttcaacctt gctggcctga atttcaaatt gttgcccctg gcccggcgac gccacgactt
     1381 tacccgtcac aatgaccgag cagccggtgg tcagacgcaa gacgtcttca ttgtaattgg
     1441 gcagagaatt attgatgaca gcctgtacag gatcaaagca ggaaccgtca taaacggcga
     1501 ggaaggagat gccagctttt gaatctcggc gggtacgtac ccatccgcgc acggtgactt
     1561 cgctgtcaac ggctacgcgg ccctggagta cgtcggctac aggcacaacg ctcataatat
     1621 tctctctgtt aatagtcgga aaaaataaac acttgtccac ccgtaaatgg ggggatacct
     1681 atgttacctg gcatctgcaa tcagacaagc agaattcgca aatgcagcga aagatttcgg
     1741 aattaaaggt aaaaaaaggg agccgattag ctccctgatg atattaactg gcttttttaa
     1801 tatgcggaag gtcgaacgct ttgcgcagcg cccgaacaaa cgctttatcg tggcagatag
     1861 tttttccagg gctgtcagaa agtttcgcca ccggtttacc gttacactct accaacttaa
     1921 tgacaatatt cagaggtttt acctggggga tatcgcaggt caggcgagtc ccaataccaa
     1981 aacttaattg cacgcgggaa gagaagtggc ggtatagctc aaccgctttg cgtaaatcca
     2041 gattgtcaga gaaaaccagc gttttactct gtggatcaat tcccagcttt tcataatgtg
     2101 caatggcttt ttcaccccat tcaaccgggt caccagagtc atggcgcagg ccctgatagc
     2161 gactggcgaa ctcgacaccg aaatcacgca ggaaagcatc catagtgatg cagtcggtta
     2221 atgcaatgcc aagttggtcg ggatactctt ccagccaggc agcaagtgca gctcgctggc
     2281 tgttggctag atccgggctg atttgctgat gcgcctggaa ccattcgtgt gcctgtgttc
     2341 ccatcggcgt gagggaaagc cgacgcgcca gatcgtagtt gctggtgccc acgaaccagg
     2401 attcctgttg cagacgtttc acgatggttt cttgtacttc gcgagaaaaa cggcggcggg
     2461 tgccaaaatc catcagatgg aagcgcgaca tatcaagacc ggcggttaat gccgagaagt
     2521 cgactaattt gttttccagc gtgtcgaggg cttgcgcaac gtcggcctgc ggtgagcgat
     2581 agcgatgtac catttcactg ataaccgcca gcaaaggaac ctcccagagg atgacttcac
     2641 gccacgggcc gcttaaacga atatccagct tgccattatc gttggacacg gtgacttgtt
     2701 ccgggttaaa gcggaactcg cgtagccagt taagatagtc ggccttaaag aaaggcaggg
     2761 cagagagcca ctgatattca tcatcctgca ggcgcaggtg ctgcatcgcc tgaacctgtt
     2821 cacgaatagc atcggcataa atacccagca gatcgtcacc tcggcaacga aactccgccg
     2881 cgacatgcac atcgtaatag tgatgaaaca cggcttgctg catatgcaac ttataagcat
     2941 ctgtatccag caacgagtgc agaacaggag aagcgaattg tgtcataggt gcgcagtagc
     3001 gtcctcgtac aggagcgttt agtacaataa acatcttcag gaactgctgg agtatacctt
     3061 gtttcgcaat ttattgaacc ccgatcacac catatgccac ctttctggtc gatggcataa
     3121 cgcggtgtat gttataaaaa tgtagcaata aaggcgtttg tacctgaaaa gatgaagatt
     3181 ctgcatagcg cgatttacgc aacaggaata gactgaacac cagactctat aaaagatgct
     3241 aaaggttatt tatgactcaa cagccacaag ccaaataccg tcacgattat cgtgcgccgg
     3301 attaccagat tactgatatt gacttgacct ttgacctgga cgcgcaaaag acggtcgtta
     3361 ccgcggtcag ccaggctgtc cgtcatggtg catcagatgt tccgcttcgt ctcaacggcg
     3421 aagacctcaa actggtttct gttcatatta atgatgagcc gtggaccgcc tggaaagaag
     3481 aagagggcgc actggtcatc agtaatttgc cggagcgttt tacgcttaag atcgttaatg
     3541 aaattagccc ggcggcgaat actgcgctgg aagggcttta tcagtcaggc gatgcgcttt
     3601 gcacccagtg tgaagccgaa ggtttccgcc atattacgta ttatctcgac cgcccggatg
     3661 tgctggcgcg ttttaccacc aaaattattg ccgataaaac caaatatccc ttcctgcttt
     3721 ccaatggtaa ccgcgttgcg caaggtgaac tggaaaatgg acgtcattgg gtacagtggc
     3781 aggacccgtt cccgaaaccg tgttacctgt ttgcgttggt ggcaggcgac tttgatgtgc
     3841 tgcgcgacac tttcaccacg cgttctggtc gcgaagtagc gctggagctg tacgtcgatc
     3901 gtggcaacct tgatcgtgcg ccgtgggcaa tgacctcgct gaaaaactcc atgaaatggg
     3961 atgaagaacg cttcggcctg gagtatgacc tcgacatcta tatgatcgtc gcggtggatt
     4021 tcttcaatat gggcgcaatg gagaataagg ggctgaatat ctttaactcc aaatatgtgc
     4081 tggcccgcac cgacaccgcc accgacaaag attacctcga tattgaacgc gttatcggcc
     4141 atgaatattt ccataactgg accggcaacc gtgtcacctg ccgcgactgg ttccagctca
     4201 gtctgaaaga aggtttaacc gtcttccgcg atcaggaatt cagctcagac cttggttccc
     4261 gcgcggtaaa ccgtatcaat aatgtgcgca ccatgcgcgg attgcagttt gcagaagacg
     4321 ccagcccgat ggcgcaccca attcgcccgg atatggtcat tgaaatgaac aacttctaca
     4381 ccctgaccgt ttacgagaag ggcgcggaag tgattcgtat gatccacact ctgcttggcg
     4441 aagaaaactt ccagaaaggg atgcagctct atttcgagcg tcatgatggt agcgcagcga
     4501 cctgtgacga ctttgtgcag gcgatggaag atgcgtcgaa tgtcgatctt tcccatttcc
     4561 gccgttggta cagccagtcc ggcacgccgg ttgtgactgt caaagacgac tacaatccgg
     4621 aaactgagca gtacaccctg accatcagcc agcgcacgcc agctacgccg gatcaggcag
     4681 aaaaacaacc gctgcatatt ccgtttgccg tcgaactgta tgacaacgaa ggcaaagtga
     4741 tcccgttgca gaaaggcggt catccggtga attccgtgct gaacgtcacc caggcggaac
     4801 agacctttgt ttttgataat gtctacttcc agccggtgcc tgcgctgctg tgcgaattct
     4861 ctgcgccagt gaaactggaa tataagtgga gcgatcagca actgaccttc ctgatgcgtc
     4921 atgcgcgtaa tgatttctct cgctgggacg cggcgcaaag tttgctggca acctacatca
     4981 agctgaacgt cgcccgtcat cagcaagggc agccgttgtc tctgccggtg catgtggctg
     5041 acgctttccg cgcggtgctg ctcgatgaga agattgatcc ggcgctggcg gcagaaatcc
     5101 tgacgctgcc ttccgtcaat gaaatggcag aactgttcga tatcatcgac ccgattgcta
     5161 ttgccgaagt acgcgaagca ctcactcgta ctctggcgac tgaactggca gatgagctgc
     5221 tggctattta caacgcgaat taccagagcg agtaccgtgt tgagcatgaa gatattgcaa
     5281 aacgcactct gcgtaatgcc tgcctgcgct tcctcgcttt tggtgaaacg catctggctg
     5341 atgtgctggt gagcaaacag ttccacgaag cgaacaatat gaccgatgcg ctggcggcgc
     5401 tttctgcggc ggttgctgca caactgccat gccgtgacgc gctgatgcag gagtacgacg
     5461 acaagtggca tcaggatggt ctggtgatgg ataaatggtt tatcctgcaa gccaccagcc
     5521 cggcggcgaa tgtgctggag acggtacgtg gtctgttgca gcatcgcgca tttaccatga
     5581 gcaacccgaa ccgcattcgt tcattgattg gcgcatttgc gggtagcaac ccggcagcgt
     5641 tccatgccga agatggcagc ggttatcagt tcctggtgga aatgcttacc gacctgaaca
     5701 gccgaaatcc acaagtggct tcgcgcctga ttgaaccgct gattcgcctg aaacgttacg
     5761 atgccaaacg tcaggagaaa atgcgcgcgg cgctggaaca gttgaaaggg ctggaaaatc
     5821 tctctggcga tctgtacgag aagatcacca aagcactggc ttaataaata accgaatggc
     5881 ggcaatagcg cctccattcg gagaatttac ccctgttttc tcaggcgagt ttcaaattca
     5941 ccacgctgca tcacccgctg caatacttcc gcttccagct ctgctaatcg taccgaccct
     6001 aaccggcgcg gacgggggat atccaccgtc aaatccagac caatttttcc ctcttcaatt
     6061 aacaacaccc ggtcagccat cgccaccgct tcgctcacat catgcgtcac cagcaaaacg
     6121 gtaaaaccgt gcttcagcca aagcgacaca atcaaatcct gcatctcgag tcgcgttaaa
     6181 gcgtccagcg caccgagtgg ttcatcaagc aacaataaac ccggtcgatg aatcagcgcc
     6241 cttgccagcg ccacacgttg tttctgcccg ccagaaagtg cggcaggcca ttcccccgcg
     6301 cgattctcca gccctaccgc agccagagcc tgacgcgccg catcgcgcca ctggccttta
     6361 aggcctaacc caacgttatc aatcaccgat ttccacggca gcagacgcgc gtcctgaaac
     6421 atcattcgcg tatcatcctg aatctcagcc agtggtgtgg tgcccgctaa cacgtcgcct
     6481 gcggttggcg tttccagacc tgccagcagg cgcagcagag tacttttgcc accgccgctg
     6541 cggcccacca ccgccacaaa ctgacctgcc ggaatatgta aatccagttg gttcaggacg
     6601 atattttccg cgtaatgttt gcttactgca ttgagcaaca atggtgtgcc ctggttcaga
     6661 cgagcggtat tcataccgtg gcctccttca aatgataagc cgggttccag cgcaaccaga
     6721 gacgctctaa cagctgcgcg ctgacgtcag caagtttgcc gagcagggcg taaagaataa
     6781 tggcgactac caccacgtcc gtttgcagaa actcccgcgc attcatcgcc agataaccaa
     6841 tgccggaatt ggcagaaata gtttcggcaa cgatcagcgt cagccacatc aggcccaaag
     6901 caaaacgcac accgaccata attgagggca gggcaccagg caggatcaca tggataaaca
     6961 gcggtatgcc ggataatcca tagctacgtg ccatctccac cagcccacgg tcgatattac
     7021 ggatgccatg ccaggtgttg atataaatgg ggaatagcgt accgagcgcc accagaaaga
     7081 tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag cgggatcagc gccagatgcg
     7141 gcacgttgcg caacatctga atggaggtat ccagcagtcg ctcaccccag cgtgacagcc
     7201 cgctaatcag ccccagaatt aatcccagcg atccgccaat tgaaaaacca atcagcgcac
     7261 gccaggagct gatcgccaga tgctgccaca gttcgccgct ggcagagagc gtccagaacg
     7321 ccatcaccac tccttccggt gagggcaaaa tacgcgtcga tagccagcca accgaggagg
     7381 ccagttgcca caccgccacg atgcccaccg gtaaaaaccc gggggcaacg cgcaataacc
     7441 acttcttcac tggcgttgcc atcatcgtgt tccttagctt tgcgcgacgt tacgggggat
     7501 aaaatcattc gccaccgctt cgccttgtgg attcagcggt tgtggctggg gaatttccgg
     7561 gatggcgaca tccagatgtg ggaacagtaa ctcgccgacc cgatacgctt cttccagatg
     7621 cgggtagccc gaaagcacaa aactgtcgat gccaagtgca gcgtattcgt tgattcgcgc
     7681 ggcgaccgta ggaccatcgc ccaccagcgc cgtcccggca ccgccgcgca ctaagccaac
     7741 acccgcccat aaattggggc tgatctccag attatcgcgc ttgccgttat gtagcgccgc
     7801 cattcgctgt tgtcctacgg aatccgttag ggcaaacgcc gcctgtgctt tggcgatagt
     7861 ttcatcatca agatgcgaga ttaaccgctc ggcggcctgc cacgcttcgt cgttggtttc
     7921 acgaacaatc acatgcagac gaataccgaa acgaattttg cgtccatgcg cggcagcttt
     7981 cgcccgcacg tgttcgattt tctcttttac cagttccggt ggttcgcccc aggtaaggta
     8041 gagatcaact tgttctgccg ccagctcctg ggcgacatct gacgatccgc caaagtaaag
     8101 cggaggataa ggctgttgaa tcggcgggaa gagcagtttg gctccgcgta catgaatatg
     8161 tttgccgttg aaatccacag tctcgccaag caacaaccgc cgccagacct gggtaaattc
     8221 tgccgaggct tcgtaacgct cgctatgatc aaggaacacg ccgtcgcctg ccagttcttg
     8281 tggatcgctg cctgtgacca ggttaaacaa cgcacgtccg ttagagagac ggtcaagcgt
     8341 ggcggcctga cgggcggcaa cggtaggtga ggttacgctg gggcgcaggg cgacaagaaa
     8401 cttcagccgt tgtgtcaccg ggatcatcga cgccgccacc agccacgcat cttcacacga
     8461 tcgccccgtc gggatcagca caccggtata gcccagacga tccgccgctt gcgcaatttg
     8521 ttgcagataa ccgtgatcaa ccggacgtga accttcttcc gttcccagat aatgcccgtc
     8581 accgtgggtc ggtaaaaacc agaacatatt cagactcata attgttttcc ttccagttga
     8641 gtgggctgcc agatgcgctg gcgaatatcg acttttttcg gcaccagacg gttttcataa
     8701 aacagatctg ccgtttgctg ctgtaaggcg gcaacttcag cgctcaacgg tttgatggtg
     8761 gtgggaggac gatgatccag atatgaggca ataaccggtg ccggtaagcc catcgttttt
     8821 gccagtaaag cgatgctttg ttcgcgctgg ctgcgggtta acgcatcggc ctcactaaag
     8881 gttgtcagca caccctgaat aaaagcgccg tttttttctg catacggacg agctgccaga
     8941 taaaacgatc cagtttgatt gagatcggtg ccgtctttca gcacccgcac gccgccctgt
     9001 aataatgcag cggagtagta gggatcccag atagcccagg cgtcaacgtt accttgctgg
     9061 aacgcggcgc gggcatcggc aggcgtcagg taagtgggct gaatatccgt gaatttaagc
     9121 ccggcctggc gaagggctcg cagtaaaaga ttgtgcgaac tggaaccttt ctgaaaggca
     9181 actttgtgac ctttaagatc ggctacggtt ttgatcgggc tgttttctgc caccagaatc
     9241 acttcggctt tgggcttcgg tggctcgacg cccacgtata ccaaatcagc cccggcagcc
     9301 tgggcaaaga ttggcggaat atccccggta ctgccgagat cgatactgcc aacgtttaac
     9361 gcctccagca tctgtgggcc agcggggaac tccacccagg agatttttgt ttgcggatag
     9421 cgtttttcca gtaactggtg gctttttgcc agcaccatac caatactgcc tttttgatag
     9481 cctatacgta acgcctctgg cgaagactcc gcggcaacag caagtgtaga gacgctaagt
     9541 aaacccgcca gcgccagttt aatgatctta cgcatgggca ttacctcgca gagacagaag
     9601 gtcaggaacc tgaacatcgc ggcggtgcaa cgcctgccag aaagtttcta gcgcggtatc
     9661 aagacgagtt tgcagatttg gcgtgaactg ggggttgtgt tggtaatcaa tgacttgcga
     9721 gtcatcggca aacacgccgt gcaggatctc ctgggctttc agcgtactta aaactggctt
     9781 aagggcataa tcgaccgcca gcagatgggc cacggtgccg cctgtcgcca gcggcagcac
     9841 cactttgcct tgcaacgcac gttctggcag caggtcgagc agggttttca gtgcaccgga
     9901 ataggcggct ttatacacag gcgtggcgac aatcagccca tcggcctgtt gcagctgttc
     9961 ggtgaaggtc ttgagtgccg gactatcgaa acgagcataa agcagatctt ccgggacgaa
    10021 gttttgcaaa ttccagtgat aaacctctac atccaggcca tttagttttt cccgcgcata
    10081 ttccagcaag gagctggagc gagaaggaaa gcgaggacta cccgccaggg tgatgacacg
    10141 catactctct ccttataacc aattgttctt gttttgttaa cattgataac aattcggtca
    10201 gtatgacaga gggcgtccgc gagtcgaaat gatttatctg aggaagaaaa gccaaaaaaa
    10261 gcataaaaaa agcgcaataa gaaaacgttt gctatttatt gccgcaggtc aattcccttt
    10321 tggtccgaac tcgcacataa tacgcccccg gtttgcacac cgggaatcca ggagagttca
    10381 tgtactaccc cttcgttcgt aaagcccttt tccagctcga tccagagcgc gctcatgagt
    10441 ttacttttca gcaattacgc cgtattacag gaacgccgtt tgaagcactg gtgcggcaga
    10501 aagtgcctgc gaaacctgtt aactgcatgg gcctgacgtt taaaaatccg cttggtctgg
    10561 cagccggtct tgataaagac ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat
    10621 ccatcgagat cggtaccgtc acgccacgtc cccagccagg taatgacaag ccgcgtctct
    10681 ttcgtctggt agatgccgaa ggtttgatca accgtatggg ctttaataat cttggcgttg
    10741 ataacctcgt agagaacgta aaaaaagccc attatgacgg cgttctgggt attaacatcg
    10801 gcaaaaataa agatacgccg gtggagcagg gtaaagatga ctatctgatt tgtatggaaa
    10861 aaatctatgc ctatgcggga tatatcgcca tcaatatttc atcgccaaat accccaggat
    10921 tacgcacact gcaatatggt gaagcgctgg atgatctctt aactgcgatt aaaaataaac
    10981 aaaatgattt gcaagtgatg caccataaat atgtgccgat cgcagtgaag atcgcgccgg
    11041 atctttctga agaagaattg atccaggttg ccgatagttt agttcgccat aatattgatg
    11101 gcgttattgc aaccaatacc acactcgatc gttctctggt tcagggaatg aaaaattgtg
    11161 atcaaaccgg tggcttaagt ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc
    11221 gcttgtcaca ggaattaaac ggacgcttgc cgattattgg tgttggcggc attgactcgg
    11281 ttatcgctgc gcgtgaaaag attgctgcgg gtgcctcact ggtgcaaatt tattctggtt
    11341 ttatttttaa aggtccgccg ctgattaaag aaatcgttac ccatatctaa ttatttcttc
    11401 aaattcctct ttataagata atcagggctt tattttcagc cctggttgtt ttatattcat
    11461 cgctgttgct tatttagaca ttttgtactt ttattgttga ggttattaag cgaagcgaca
    11521 atggattgtg tcgctgcggt tttataaggt tggggagtgg cagatgcgaa ttaaaccaga
    11581 cgataactgg cgttggtatt acgatgaaga gcacgatcga atgatgctcg atttagccaa
    11641 tggtatgcta tttcgctcac gttttgcgcg caaaatgttg actccagatg ccttttctcc
    11701 cgcaggtttt tgcgttgatg acgccgcgct ctatttctct tttgaagaaa agtgccgcga
    11761 ttttaattta tctaaagaac aaaaagccga actggtgctg aatgcactgg tagcaattcg
    11821 ttatctcaaa ccgcaaatgc cgaaaagctg gcattttgta tctcatggtg aaatgtggat
    11881 gcccatgccg ggcgatgcgg cctacgtttg gttgagcgac actcatgagc aagtgaattt
    11941 actggtcgtt gaatctggcg agaatgccgc gctatgtcta ctggcacaac cctgcgttgt
    12001 tattgcgggt cgcgcgatgc agttgggcga cgccattaaa atcatgaacg acaggctgaa
    12061 accgcaggtt aatgttgaca gcttcagcct cgaacaggca gtctaacgcg ccagcttaag
    12121 cgcagtcttc ggtacacaac tacagcaaag aatagtgcca tcatcgccta ttgctgattt
    12181 ttttagcggc gtgacttcgc cttctaaaag ctgaacacgg caacttccac aaatgcccgc
    12241 gcggcaagaa taagggatac gaattccctg attttctaat tgctccagca gcacctgttg
    12301 gttatttcca cgaaatgcct gtccctgcca gtcaatatct acgtttgcgt ccggttgttg
    12361 cgtggtgttg gcagtatcat cagcggcacc tgcgccgtaa attttagccg gagccgttgc
    12421 cagaatttcc acctcgtcgc caacgcgaat cacgccgcta ttacgggcaa ttaaattctg
    12481 accaaaatcg acatcgccgt tatcctgggc agtacggaaa gattgcagcg tttttaatgg
    12541 ttcgcctgcc ggatgttttt gccctttttc tgggctgacg gtggtgaaaa tacagcggct
    12601 acaaggctta accacatcaa acacgacatc accaatgcga atcactttcc agctatcttc
    12661 ttcccaggcc gatgcaccgg aaaccaccag attggggcgg aattgctcca tttttacact
    12721 ggccggacaa cgttgttgga gatcacgtaa cgaggcctcg ttagcaagaa ggtaaggata
    12781 gccatcagca aatgacaggg gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg
    12841 ccccacccag cgtaattgca cttcgcggga gaaaaatcca cttagccatt tgttgatcgc
    12901 gtctggcgca attcgcgcgg taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt
    12961 ggcgaaatca gcaaaacgaa catatgcact actgccatct ggtgcggtga gatgcaagcc
    13021 atcatgcacg ggtgaaggag taaaccgtac cattagcgga aactggcgag cggtaataaa
    13081 cgtaccgtca ggttccgtga tcataaagat gcgatcgaag gccagaccac tgacatctgc
    13141 cagggtatgt gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa aaagccgggt
    13201 taatgtcgcc acggtgcggt cctcaaatga aaataagccc tcaactttat gacatgacgc
    13261 gcttattagc tataatgcgc aacaattttc ttagagtaaa agtgacaata tgaattctct
    13321 gtttgccagt acggcccgtg ggctggaaga gctgttaaaa actgaactgg aaaacctggg
    13381 ggccgttgaa tgccaggtgg ttcagggtgg ggtccatttc aagggcgaca cacggcttgt
    13441 ttaccagagc ctgatgtgga gccgcctggc ctcgcgtatt atgttgccgc tgggcgagtg
    13501 taaggtttac agcgatttag acctctatct cggtgttcag gcgatcaact ggacagagat
    13561 gtttaatcct ggcgcgacct tcgctgtcca cttcagtggt ttgaatgaca ccatacgcaa
    13621 cagtcagtac ggtgcgatga aagtgaaaga cgcgatcgtc gatgctttca cgcggaaaaa
    13681 tctgccgcgt ccaaatgttg atcgcgatgc gccggatatc cgcgttaacg tctggctgca
    13741 taaagaaacc gccagtatcg cccttgatct cagtggtgat ggtttacatc tgcgtggcta
    13801 tcgcgatcgt gctggcattg cgccgatcaa agaaaccctg gcagccgcta ttgtgatgcg
    13861 atccggctgg cagccaggaa caccgctgct cgatccaatg tgtggttcag gtacgttgct
    13921 gattgaagcg gctatgttgg cgaccgaccg cgcaccgggc ttgcaccgtg ggcgttgggg
    13981 ctttagcggc tggacgcagc atgatgaagc tatctggcag gaagtgaaag cggaagcgca
    14041 aactcgtgcc cgtaaaggcc tggcggagta tagctcccat ttctacggtt cggacagtga
    14101 cgcacgggtg attcaacgtg cgcgcactaa cgcccgtctt gcggggattg gtgaactgat
    14161 cacctttgag gtgaacgatg tcgcgcaact ggccaatccg ctgccgaaag ggccgtacgg
    14221 tacagtgttg agcaacccgc catacggtga acgtctggac agcgaaccgg cgctgattgc
    14281 gctgcatagt ctgctgggcc gaatcatgaa aaaccagttc ggtggctgga atctctcttt
    14341 gtttagtgcc tcgccggatc tgctaagctg cctgcagctg cgtgcagaca aacagtacaa
    14401 ggcgaaaaac ggcccgctgg actgcgtaca gaaaaactac catgttgccg aaagcacacc
    14461 agacagcaaa ccggtaatgg cagcggaaga ctacgccaac cgtctgcgta agaacctcaa
    14521 aaaattcgag aagtgggctc gccaggaagg aattgaatgt taccgcctgt atgacgccga
    14581 tctgccggaa tataacgttg ccgttgaccg ttatgccgac tgggtggtgg tgcaggagta
    14641 tgcgccgcca aaaactattg atgcgcacaa agcgcgtcag cgtctgttcg atattatcgc
    14701 tgcaaccatt tcggtactgg ggattgcgcc aaacaaactg gtgctgaaaa cccgtgaacg
    14761 ccagaagggc aaaaatcaat accagaaact gggcgagaag ggcgagtttc tggaagttac
    14821 cgaatataac gctcacttgt gggtgaacct gacggattac ctcgataccg gtctgttcct
    14881 cgatcaccgt atcgcccgtc gtatgctcgg tcagatgagc aaaggcaaag atttcctcaa
    14941 cctgttctct tataccggca gcgccaccgt gcatgcggga ttaggcggtg cacgcagcac
    15001 caccaccgtg gatatgtcgc gtacttatct ggagtgggca gaacgcaacc tgcgtctgaa
    15061 tggcctaacc gggcgtgcgc atcgcctgat tcaggccgat tgcctggcgt ggctgcgtga
    15121 ggcaaatgaa cagtttgatc tgatctttat cgatccgcca accttctcta actcaaaacg
    15181 aatggaagat gcgtttgatg ttcagcgcga tcatctggcg ctgatgaaag atttgaaacg
    15241 tctgctgcgt gcaggtggga cgatcatgtt ctcgaacaac aaacgcggct tccgtatgga
    15301 tctcgacggc ctggcgaaac tgggactgaa agcacaagaa attacgcaaa aaacgctctc
    15361 ccaggatttc gcccgtaacc gccagatcca caactgctgg ctgattaccg cagcctgaaa
    15421 ggaatagtaa tgtcattaat cagtatgcat ggcgcatggc tgtcgttcag cgacgcgccg
    15481 cttctcgata acgcagaact gcatatcgaa gataacgaac gtgtttgtct ggtggggcgt
    15541 aacggtgcag gcaaatcaac gttaatgaag atcctcaacc gtgaacaggg gctggatgac
    15601 ggtcgcatta tttatgagca agatttgatt gtagctcgtc tgcaacagga tccgccgcgt
    15661 aacgttgagg gtagcgttta tgatttcgtt gccgaaggca ttgaagaaca agcggaatac
    15721 ctgaaacgct atcacgatat ttcgcgcctg gtgatgaacg acccgagcga gaaaaatctc
    15781 aacgaactgg cgaaggttca ggaacagctg gatcaccaca acctgtggca gctggaaaac
    15841 cgcatcaacg aagtgctggc gcaactggga ttagatccta atgttgcgct gtcgtcgctt
    15901 tccggcggct ggttgcgtaa agcggcatta ggacgcgcgc tggtgagcaa tccgcgcgta
    15961 ttgctgctcg acgaaccgac taaccacctg gatattgaaa ccatcgactg gctggaaggg
    16021 ttcctcaaaa ccttcaatgg gacaattatt ttcatttccc acgaccgttc gtttatccgc
    16081 aatatggcga cgcgcattgt tgatctcgat cgcggcaagc tggtgaccta tccagggaat
    16141 tacgaccagt acctgctgga aaaagaagaa gccctgcgcg tggaagaatt acaaaatgcc
    16201 gagttcgatc gcaaactggc gcaggaagag gtgtggatcc gccaggggat caaagcacgt
    16261 cgtacccgta atgaaggccg cgtacgcgcc ctgaaagcaa tgcgtcgcga gcgtggtgaa
    16321 cgtcgcgaag taatgggtac cgcaaagatg caggtggaag aagccagccg ctccggtaag
    16381 atcgttttcg aaatggagga cgtttgctac caggttgacg gtaagcaact ggtgaaagat
    16441 ttttctgccc aggttctacg cggcgacaaa attgccctga ttggtccgaa tgggtgcggc
    16501 aaaaccacgc tgctgaaact aatgctcggt cagcttcagg cggacagcgg gcgtattcac
    16561 gttggcacca aactggaagt ggcttatttc gatcagcacc gtgcggaact ggatcccgat
    16621 aaaacggtga tggataacct tgctgaaggt aagcaagagg tgatggttaa cggcaagcca
    16681 cgtcatgttt tgggctattt gcaggacttc ctgttccatc caaaacgggc gatgacgccg
    16741 gtacgtgcgc tttctggcgg tgagcgtaac cgcttgttgt tggcgcgttt gttcctcaaa
    16801 ccaagcaact tattgattct tgacgaaccg accaacgatc ttgatgtcga aacgctggaa
    16861 ctgctggaag aactgatcga cagctatcag ggcacggtat tgctggttag ccacgatcgt
    16921 cagtttgtcg ataacaccgt tacagaatgc tggatcttcg aaggcggcgg taaaattggt
    16981 cgttatgtcg gcggttatca tgatgcccgt ggtcagcaag aacagtatgt ggcgctcaaa
    17041 cagcctgcgg tgaaaaaaat cgaagaagcc gccgcgccaa aagcagaaac tgtaaaacgc
    17101 agcagtagca aactaagcta taaattgcag cgcgaactgg agcagctacc gcaattgctc
    17161 gaagatctgg aggcgaagct ggaagcccta cagacgcaag tggcggatgc ttccttcttc
    17221 agtcagcccc atgagcagac acaaaaagtg ctcgccgata tggctgctgc agagcaggag
    17281 ctggagcaag cctttgaacg ctgggagtat cttgaagcgt taaaaaatgg tggctgatcg
    17341 caaaatagtc gatttaaaca gagacagctg acaacggtaa aattgcctga tgaactgggt
    17401 ttatcaggca tgaaaaccaa cactttgttc gtcatctgta aaatagcgca tcattaagga
    17461 gtaccaatgt gcgaacatca tcatgccgcg aagcacatcc tgtgctcgca gtgtgacatg
    17521 ctggtggcgt taccgcgcct tgagcatggt cagaaagcgg catgtccccg gtgtggcaca
    17581 acgttaaccg tggcgtggga tgcccctcgg cagcgtccca ccgcctatgc gttggctgca
    17641 ctgttcatgc tgttgctgtc caacttgttt ccttttgtga atatgaacgt tgcaggagtt
    17701 accagtgaaa ttacattact ggaaattccc ggcgtgcttt tttctgagga ctacgccagc
    17761 ctcggcacct ttttcctgtt gtttgtgcaa ctggttcccg cgttttgtct gataaccatt
    17821 ctgttactgg tgaatcgcgc ggaattaccg gtccgtttaa aagagcaact ggcacgggtg
    17881 ctttttcaac tcaaaacctg gggaatggcg gagattttcc tcgcgggtgt gctggtcagt
    17941 ttcgtgaaac tgatggctta cggcagcatt ggggtaggca gcagctttct cccctggtgt
    18001 ttattttgtg tcctgcaact gcgcgctttt cagtgtgttg atcgtcgctg gttatgggac
    18061 gacatcgccc cgatgccaga actgcgccag ccgctaaaac caggtgtcac ggggatacgt
    18121 caggggctgc gttcgtgctc ctgttgtacg gcaatccttc ctgctgatga acccgtgtgc
    18181 ccgcgttgta gtaccaaagg gtacgttcgg cgtagaaaca gcctgcagtg gacactcgcg
    18241 ctgcttgtaa cgtccatcat gctgtatctt ccggctaata ttttgcccat catggtgacg
    18301 gatttattag gctcgaagat gccgtcgacg attctcgctg gggtcattct gttatggagc
    18361 gaaggctctt atcccgtcgc tgcggtgatt tttctggcca gtattatggt gccaacgtta
    18421 aagatgatcg ccattgcgtg gctgtgttgg gatgccaaag gacacggcaa gcgcgacagt
    18481 gaaagaatgc atttgattta tgaagttgtt gagtttgtag gccgctggtc gatgattgac
    18541 gttttcgtta tcgcggtgct ctcggcgctg gtgcgtatgg gaggtttaat gagtatttat
    18601 ccggcaatgg gtgcattaat gtttgcttta gtcgtcataa tgacaatgtt ttctgctatg
    18661 acgtttgacc cgcgtttgtc gtgggatcgt caacctgaat cagagcatga ggagtcctga
    18721 cagtatggaa tctaataatg gggaagccaa aatccagaaa gtgaagaact ggtctcccgt
    18781 gtggatattt cctatcgtca cggcgctcat tggggcctgg gttctttttt atcattacag
    18841 ccatcaggga ccggaagtga ccctgatcac cgcgaatgcg gaaggaattg aaggtggcaa
    18901 aaccaccatt aaaagccgta gcgttgacgt cggcgtggtt gaaagcgcca cactggctga
    18961 tgatttgacg cacgttgaaa tcaaagcgcg gctgaattcc ggtatggaaa agttgctgca
    19021 taaagatacc gtcttttggg tggtgaaacc gcagattggt cgagaaggga ttagcggcct
    19081 gggaacactg ctgtctgggg tttatatcga actgcagcca ggcgcgaaag gcagcaaaat
    19141 ggataaatac gatttgctgg actcgccacc gttggcccca cctgatgcga aaggtatccg
    19201 tgtgcttctc gatagcaaaa aagccgggca gctctcgcca ggagatccgg tgctgttccg
    19261 tggctatcgg gtaggttcgg ttgaaaccag cactttcgat acgcaaaaac gcaatatcag
    19321 ttatcaactg ttcatcaatg caccttatga ccgactggtg accagcaatg ttcgtttctg
    19381 gaaagatagt ggcattgcgg ttgatctgac gtcagcggga atgcgtgtgg agatgggctc
    19441 attgacaacg ctgcttagtg gcggtgtcag ctttgatgtg ccggaaggtc tggatttagg
    19501 gcagccagtg gcaccgaaaa cagctttcgt tttgtatgat gatcagaaga gcattcagga
    19561 ttcgttgtac accgatcaca ttgattatct gatgttcttt aaagattcgg tacgcggtct
    19621 gcaaccggga gctccggtag aattccgggg tattcgcctg ggtaccgtaa gcaaagtgcc
    19681 attctttgcg ccgaatatgc gtcagacatt taacgatgat taccgtattc cggtactgat
    19741 tcgtatcgag ccagagcggc tgaaaatgca gcttggcgaa aatgcggatg ttgttgagca
    19801 ccttggcgaa ttgttgaaac gtggtttacg cggatcgctg aaaaccggaa acctggtcac
    19861 tggcgcactg tatgttgatc tcgatttcta tccaaatacg cctgcaataa ccggtattcg
    19921 tgaatttaat ggttatcaga ttatcccgac cgttagcggc ggtctggcgc aaatccagca
    19981 acgactgatg gaagcgttgg ataagatcaa caaactgcca ttgaatccga tgattgaaca
    20041 ggcaaccagt acgctttctg aaagtcagcg cacaatgaaa aacctgcaaa cgacgctgga
    20101 tagcatgaac aagatcctcg ccagccagtc gatgcagcag ttaccgacgg atatgcagtc
    20161 aacgttgcgt gaattgaatc gcagtatgca gggcttccag cctggctccg cagcctacaa
    20221 caagatggtg gcggatatgc agcgacttga tcaggtgttg cgagaactgc aaccggtgct
    20281 gaaaacgctc aacgagaaga gtaacgcgct ggtatttgaa gcgaaggaca aaaaagatcc
    20341 agagccgaag agggcgaaac aatgaaaaag tggctagtga cgattgcggc actgtggctg
    20401 gccggatgca gctccggcga aattaataaa aactattacc agttacctgt ggtgcagagc
    20461 ggtacacaaa gcaccgccag ccagggtaat cgcctgttat gggttgagca ggtcgctgtt
    20521 cctgactatc tggcggggaa tggtgtggtt tatcaaacca gtgatgtgaa gtatgtgatt
    20581 gccaacaaca acctgtgggc cagcccgttg gatcaacagt tgcgcaacac cctggttgcc
    20641 aacctgagta cgcaactgcc cggctgggtg gttgcctccc agcctctggg aagcgcccag
    20701 gacacgctca atgttaccgt cacggagttt aacggtcgct atgatggcaa ggtcattgtc
    20761 agtggtgagt ggctgttgaa ccaccaggga caactgatca aacgtccgtt ccgtctggaa
    20821 ggagtgcaaa ctcaggatgg ttacgatgag atggttaaag tgctggccag tgtctggagt
    20881 caggaagccg cttctattgc gcaagagata aagcgtctac cttaatcata aagatttgta
    20941 aatataaccg tctccggtat gttgcctgag gcggtttttt tgtttctaac gtgcggaaaa
    21001 agttgttcct cttcacattt tttgtacaac cgacatgccc gtgtagctca caaatatgac
    21061 agtggcgtga attttgcgca ttgacggcag ttatgattcg cggtattgct taactgtgat
    21121 tacatattta gtaatcactg ttttcttttc caccagaaac cagtatgagg gaaacgaggc
    21181 atgaagagac aaaaacgaga tcgcctggaa cgggcacatc aacgtggtta tcaggccggc
    21241 atcgccggac gctcaaaaga aatgtgtccc tatcagacgc tgaatcaaag gtcacaatgg
    21301 ctgggaggct ggcgagaagc catggcggac agggtagtaa tggcctgatt ctgtctcttt
    21361 aaaaagaaac ctccgcattg cggaggtttc gcctttatat actctgtctg attaaaatca
    21421 gaatgcagac gtatcctgga acagacctac tttcaggtcg ttggcggtat agatcaaacg
    21481 accatcaacc agcacttcgc catcagccag gcccataatc agacgacggt taactatgcg
    21541 tttaaagtga atacgatagg tcactttttt cgccgtcggc agcacctggc ctgtgaattt
    21601 cacttcacct acgcccagcg cgcgaccttt accttcgccg cccagccagc cgaggtagaa
    21661 ccctaccagc tgccacattg cgtccaggcc caggcatccc ggcataaccg gatcgccgat
    21721 aaagtggcat ccgaagaacc acagatccgg attgatatcc agctctgctt caacataccc
    21781 tttgtcgaag ttaccacccg tttcggtcat tttgaccaca cggtccatca tcagcatgtt
    21841 tggtgctggc aattgcgggc ctttagcgcc aaacagttca ccgcgaccag aggcaagaag
    21901 gtcttctttt gtataggatt cgcgtttatc taccatgttc tctgtaagcc ttattttatt
    21961 gaagcacgca ggatagctaa cacgtgtacg ccgaacaagt ccgatcagtt cggaataaac
    22021 cagttcagcc aacgtaatgg ccatggaaaa cggtgacgtc cttcctgttg cgatgcttgt
    22081 gcgatacgtt cctggatggt ttgcatcagc gtcgtttggc cttcgccatc ccacaccaga
    22141 tttaataata acggcagtgc gtcagtcaca tcgtctactg cccagatggt gaatttgtct
    22201 tcttctaccg ctttcaccag ttcactgtga agacttaaat ggcgaacgtt agctgtgggg
    22261 ataatgacac cttgtttccc cgttaactca cgttgctggc aaatagcaaa gaagccttcg
    22321 attttctcat ttaaaccacc gactggctgg gcgcgaccga actgatcgac tgaacctgtg
    22381 atagcgatac tctgattcac cggcacatcg gcgagggcgc ttatcagggc gcagagttca
    22441 gccatcgagg cactatcgcc atccacttca ctgtatgact gctcaaatgt cagcgatgct
    22501 gagaagggga tctgttgctc aagctgtagt tccgacatca ggaacgcttg catgatcatc
    22561 atccctttcg catggatatt gccgccaagc tccgctttgc gttcgatatc ggtgaattca
    22621 ccatcgccaa tatgcacaac gcagctaatg cgagaaggtt cgccaaaagc gcgtggatga
    22681 cccggaaatt caatgaccga aagggcgtta atttgcccga tgcgttcgcc ttcggtttca
    22741 atcaggattt gctcctgaag gatctcatcc tgcatgcgtt cagcgaggaa accttcgcgc
    22801 cattcacgct gctgtagcat taagtttagc tgctcgccgg agaaggtgtc gccatcacac
    22861 agggaggcga cctcttgaca ctggcggagg atccactgcg ggctaagcgg aagtgtttct
    22921 tgttcaccag tgtagcgtgc tgcttcgcgg ataagtaccg gccaggcatc cgctcccggt
    22981 gctggtaagt gattatgtct ggcggtaaat gtcacccagc gacaccactg gcttactgac
    23041 tccgcatcga caatctgcag agtatcttca aattcgctat aaatagcctg ctctgaaagc
    23101 tctggctcca tctcctggaa atcagccaat gattcgcgtt cgcctaccag aatgactttc
    23161 agcttcaatg gcatcgaagg cacagagacg gggagagggc gcgactcatc aaacgcaacc
    23221 cagtcaaaac gctcgcggtt aacgatattt ttcagccgca tccacagcag aggttgcgcc
    23281 agcagtgtac gcaaagagat gatgagaata ccgccatttg cctgatgcac cagaccaggc
    23341 tgcagggtaa tatcgccatt aaactgacgc aggcagccaa agagttgctc tgcttctacc
    23401 cagtcggcag cgacaacttg cgttaaagtc gcaaaattat catctgcact cactgcgtga
    23461 cgtaagcgga tggtgtggcc ggaaacctca tagtgaccgc ccaccagttg gcctgcatcg
    23521 ctttgtagcg tacgcgcggc atcagcaata agattcagat actcagactc ttccggggcc
    23581 ttcgccagca taaaagagga ggatgctcgc gtatgcagca actgctccag cgcaaattgc
    23641 agtcgcggtt gagtatcact gaataaagga tcgttttcgt caatcaaatg tggctgagca
    23701 aatatttcct gatagctatc ggtatcagga accaggtcac gccatgcaag tttcgtaatg
    23761 gtcaaagttg atgtttttta gtctgttgtc aaagccgcga ttataccgta accggcactg
    23821 cagcacacgt agaaagcaac gacaatactc ctggcatggg cgttaaagct cacaggatgg
    23881 agattctttt cttcactggc ctaaaaagct gatattctgt aaagagttac acggtaacat
    23941 tgagatcgct atgaaatatc aacaacttga aaatcttgaa agcggttgga aatggaagta
    24001 tctggtgaag aagcaccgcg aaggggagtt aatcacccgt tacatagaag ccagtgccgc
    24061 ccaggaagcc gtcgatgagt tgctctcgct ggaaaatgaa cccgtgctgg taaatggttg
    24121 gattgacaag catatgaatc cggaactggt caatcgcatg aagcagacca ttcgggccag
    24181 acggaagcgc cattttaatg cagaacacca gcatacgcgc aaaaaatcga tcgatctgga
    24241 atttatcgtc tggcaacgtc tggctggcct tgcgcagcgt cgcggtaaaa cgctttctga
    24301 aacgattgtt cagctgattg aagatgcgga aaacaaagag aaatacgcga ataaaatgtc
    24361 ttctttgaag caggatctgc aggcattgct gggtaaggaa taactgtcaa aagtcagttc
    24421 aatttactaa aggcgaaaaa aaaaccccgc agctgcgggg tttttctacc agacgagaac
    24481 ttaagcctgc ggctgagtta caacgtcttt gatgccttta acttcgatct ctacgcgacg
    24541 atccggagcc aggcaatcga tcagtgcagc acgctgtttc acgttgtcac aggtgttgcc
    24601 agtaaccggg ttggattcgc ccataccacg tgcggagatt ttgtctgccg ggataccttt
    24661 agagatcagg taatcaacaa cggactgagc acgacgctcg gacagagcct ggttataagc
    24721 gtcagaaccg atgcggtcag tgtaacccag aacaactacg gaaccgtctt tcggatccag
    24781 gttgctcagc tggctgtaca gctgatccag agcagcctga ccttccggtt tcagggttgc
    24841 tttgttgaag gtgaacagaa cgtcagactt cagagtgaag tgcttggtct gtacttccgg
    24901 tgccggagct ggagccggag caactactgg agctgcttcg ccctgaccga aacggtagga
    24961 aacacccaag ctcagcatgc cgttgtccgg acgagtgccg atggtgtgtg cgtcaccgat
    25021 gttgttggtc cactggtatt ccagacgggt agcgatttca ggagtgatcg catactcaac
    25081 accgccagcg aagaccggag aaacgccggt gtcgtggttt ttaccataaa cgttggattt
    25141 agtgtctgca cgccatacca taccacccag acgagtgtat acgtccagat cgtcagtgat
    25201 tgggtaaccc agtttagcgg tcagctgaac gccctgagct ttatatgcac cgttttcaac
    25261 gctgcctttg tatggcatac gacctaacca gtcataaccc atttcaaagc caacatacgg
    25321 gttaacctgg taaccaccaa aagcaccagc gcccagttgg ttttcatggg tcggaccatt
    25381 gttgttgatg aaaccagtgt catggtactg ggaccagccc agtttagcac cagtgtacca
    25441 ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa ccagccagtg ccactgcaat
    25501 cgcgatagct gtctttttca ttttttgcgc ctcgttatca tccaaaatac gccatgaata
    25561 tctccaacga gataacacgg ttaaatcctt caccggggat ctgctcaata ttaactctac
    25621 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    25681 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtacgttta
    25741 atcttttttg tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct
    25801 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    25861 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    25921 tcgttgcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    25981 ttgccgcatc aacaagttca gcatgctctt ctgcagtcaa atcatctgcc aaccaaccga
    26041 tcaccacgct gtaattgccc gtgcgtaaag cgcgaaccat tgactccacg gtgtggcaag
    26101 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    26161 cgcgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt
    26221 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    26281 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    26341 tggatgagaa cgacgaatca cgatgtgcat agcctgcagt gtacataatc aatccagccc
    26401 ctgtgagtta ctgtatggat gtacagtaca tccagtgacg acaaagatca actctatttt
    26461 cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta atcttgcccc
    26521 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    26581 ttttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    26641 caagaatacc tggcaacgtt gggcacaatt gaataccgat cattgtttgg tagttacagc
    26701 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    26761 tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag
    26821 tgtggccgat ccgttaccct caattactat cgggttgatg aaagtctatg gcgaaatcaa
    26881 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    26941 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    27001 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    27061 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    27121 ggggccatta tcggtattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    27181 gaatgggctg actctcttac gccaaaacag gagtttcctg cggaacttga gtaatctggc
    27241 gttgcagacg accaatctct ggcagtaatg caatcaataa cccaacttgt tgtacgacca
    27301 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggccagcta
    27361 atgcctgatt gacgcgttct tcatcagcag gttgatgatg tagcgcgtca tcaacatagc
    27421 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    27481 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    27541 cgcgaatttg cggggtaacg ttcggttcgc tggacatatt tgataccacc gacgccagtt
    27601 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    27661 cctgatggta ttgttccagt atggcatcga gataccggca gttggcctct gtggcgcgtt
    27721 cgagcattcg cggcagattg cgaaacttcc agtcaggcca gatgtagctc actgccgccc
    27781 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgccact tcaaaacctt
    27841 cacccagcaa gttgaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgggcgt
    27901 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accaactgcc
    27961 cttccagtga tggcacaaac cacagcacag gaataccaat tgcgatacct accagcgtac
    28021 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    28081 aacttgtcag caagatccaa tacccgtgat gcattccggt tatctgaatg atggcgtagc
    28141 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    28201 agtgacggct aagacgcagc cagatatcac tcaatccgtg cgggctgtca tcagcgagct
    28261 cattttcgtc attattatgg ggcagtgcct gggcctgttc tgattcaatt gttgccagtt
    28321 gggcatcaat ggcacgtaaa ttgttcagta aaaatcccag tgttttgagt aaatcagcag
    28381 gagcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    28441 aatgcggatc atgttgataa ggctgacgca gcaaaataca gcgtgacagt tgctggcacg
    28501 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    28561 gctcacgcaa tgtttgatat tgaatatgag aagagctggc acgctcgtga atatcctgtg
    28621 cgacaaagta ataatgcagt gtgcgacgcg ttcctcgttg accacgatcg ccacgtaagc
    28681 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagctgaccg ttggcgagag
    28741 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    28801 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    28861 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    28921 gaagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    28981 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    29041 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    29101 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    29161 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc ccaagcgtca
    29221 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    29281 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    29341 gacttagcat aaaggccata ttagtggatt attggaaacg acgacgtgca ttggcttcgc
    29401 gcgcagcttg cgctgtttct accgatacca cgcgacgacc gaccggccat agtgcaatag
    29461 cggcaatttt aaagttcgca ataccgacag gaatgccaat gatggaaata cattgtgcga
    29521 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    29581 tcaataccgt accgccagta tttagcagca catttttgcc agccgggttc agttcatcga
    29641 catgaatagc ttcattgcca taaggcacca gagacagttt ggtgatctcc cagcaggatc
    29701 gtgtcagtgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca
    29761 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    29821 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    29881 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    29941 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    30001 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    30061 caattactgg cgattcactg taagcgcggt ctggtatggg gcgagctgga atttgtactg
    30121 ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac
    30181 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggc
    30241 gttttacgtc agcaactgga tttgattgcc acgcgcaccg gagaaaataa atggctgacg
    30301 cgtgagcaaa cctctggcgt gcagcaacaa atccgccagg ctttgtcggc gttaccattg
    30361 ccggttaacc gactggaaga attcgataac tgccgtgagg tgtggcgtaa atgtcaggcc
    30421 tggttgaagg acattgaagg cgctcggttg cagcataatc aggcgtatac cgaagccatg
    30481 cttaccgagt atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag
    30541 gcccgggcag tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga
    30601 aaaacgtcgg tgctggtggc ccgtgctggc tggttactgg cgcgtggtga agcgtcccct
    30661 gagcaaattt tattgctggc gtttggtcgc aaagccgctg aagagatgca cgagcgtatt
    30721 cgagaacgac tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat
    30781 attattcagc agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct
    30841 gcccgtcatg aactttttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag
    30901 gcgaagggct ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaat
    30961 ttctgggatg atgaaaaatt acagcgtcgt cttgcctctc gcctcgatcg ctgggtaagt
    31021 ctgatgcgga tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt
    31081 cgcgatctgt tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt
    31141 gcgctgaagg cagaaaacgc tgtcgatttt tcgggcctta ttcaccaggc gattgtgatt
    31201 ctggagaaag gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat
    31261 atctcgccgc agcgtgcagc gttgttagcg gcattacgca agcaaaatag tcaaacgacg
    31321 ttgttcgctg ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg
    31381 cttaccacgg ctttccatga aaactttggt gaaggtgatc gctgtgattt agacacgacc
    31441 taccgtttta acagtcgtat cggtgaggtg gcaaacaggt ttattcagca gaacccaggc
    31501 cagcttaaaa agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg
    31561 gatgagggtc aacttgacgc tttgctggat aaactctctg gttatgccaa accggaagag
    31621 cgcattctga tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcagca
    31681 acacgctggc cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag
    31741 gcggattacg tcatcatcgt tggcttgcag gaggggagtg atggttttcc ggctgcggcg
    31801 cgggagtcga ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa
    31861 gaacggcggt taatgtacgt ggcgctgacc cgggcacgtc atcgggtatg ggcactgttt
    31921 aacaaagaga atccctctcc ctttgtggat atactgaaaa atctggatgt gccggtggcg
    31981 agaaaaccgt aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtctg
    32041 cgagataacg ctgataatcg gggatcagaa tatcgaccgc gtcgttaaaa tgcggtgact
    32101 ggataatgaa gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgttgcca
    32161 gacgcagcaa ggctttcacg tcaggatcgt gcggcacggc atttagtggg tcccagaaga
    32221 aaatcaatac atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca
    32281 ttgggccact caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg
    32341 tagtgcctgt tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc
    32401 tcatcagcat ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgaaggta
    32461 aagtgcgagt cgtcagttcc ataatgtaca tccgtagtta actttcctac agattactgt
    32521 aagtacttat cgctgcaaga taaagactga aaaagcctgc gcacaggcac aaaaatctca
    32581 ggaggatggt tgtttttccg cccattgcag gaaagtattt cgcgtttgtg ggtcagccag
    32641 tttaaaccaa tacttcagcc gttgttctgt gagcacctga gactgcggcg gaatagcatc
    32701 cagctcggaa acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg
    32761 tagtgaagcg cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt
    32821 ttttgcagtt gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc
    32881 atccaacaat tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag
    32941 gcgaggcagg cgaaaattga cctggttgat gagctgggta ttaaaactaa ccaccagtgg
    33001 aggggagata tagagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa
    33061 cactaactga tgcggtccat tgtccagttc aatgctgtcc gcaccgcgca gcagagagct
    33121 ggaaaccttc ttaccgtcga gtaccagaag atcgacatca gtagaaagcc gcagcgtggt
    33181 ggcaaaaacg gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat
    33241 agatagctcc tgttaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat
    33301 acttactaac atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat
    33361 ggttggcatg tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga
    33421 aaccgatatt gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga
    33481 caaacccgat cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcacgt
    33541 cattcctgtt tcgccaaaag ttgccggcaa aacactgctg ggccagcagg ggtatggcac
    33601 gctggcagac gttccggaaa aagtcgatat ggtcgatgtt ttccgtaatt cagaagcggc
    33661 gtggggcgtg gcgcaggaag ctatcgccat tggcgcgaaa accctctgga tgcaactggg
    33721 ggtgattaat gagcaagcgg cagtgctggc gcgtgatgca gggctaaacg tggtgatgga
    33781 tcgctgcccg gcgattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag
    33841 gcaaaaacct tccggggatt tgttcaggga ttagttacgc agacgcgggg cctggagttg
    33901 tttgcggatg gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa
    33961 ttcgctactt agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcatcctc
    34021 catcaccacg tgataccacg gagcggcgcg aagctcgtca ttcaccgcca gctcatcagg
    34081 cgacggttca gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa
    34141 cagggaatgg cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg
    34201 ggaaatcttt acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca
    34261 ggcaaaccct ttcagatata gcccttccgg gtaggtagcg atcaccggat gatcggctgc
    34321 ctgacggaac tgctctataa attgtacatc acgaccggca tcaattgcgg catccgcgat
    34381 gattttctga aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc
    34441 gccttcattc agcaactgaa tcgccagcat gttgatgtct ttataaccac gacacgcgcc
    34501 catcaactgg cttttattct caacaaactt cggcgggtcc atcacgataa catcaaattt
    34561 ttcaccgcga tcgcgataag tacgcagcaa tttaaagaca tcatcacgga caaactctgc
    34621 cttgctcaga tccagtttgt tcagctcaac gttctgccgt gcaatatcca gcgcttcgtg
    34681 ggaggtatca acgctgacaa cctggctgca accgcccatc aatgccgata cggcgaaacc
    34741 accggtatag gaaaaacagt taagtacgcg tttattttcg acgtagcggc gggtagccag
    34801 gcggctgtca cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag
    34861 cagtttcatg ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgatggg
    34921 gccctgagtc agctccatcc cttctttttt acgtaccgcg acgtcgctgc gatcgtaaat
    34981 agcacattcc gggtacagcg tttgcagggc actaattaat gccgcgcgct gatactctgc
    35041 gccagcactc agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg
    35101 caggccatca gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag
    35161 ccagtcacgc catttttgtg cttgctgtaa acggcgggta aaaaaagcaa tgtcgataga
    35221 ctcagacggg tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc
    35281 gcgtgctaac cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt
    35341 accttccatg cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt
    35401 ttcgcgccct ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa
    35461 gaaatgggcg tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag
    35521 gagagcagca atgtcgaaag tctgcataat tgcctggatt tacgggcgag ttcagggcgt
    35581 aggattccgc tacaccacgc agtacgaagc gaaaaaactg ggactaaccg ggtacgccaa
    35641 aaatcttgac gatggcagcg tggaagtggt tgcctgcggt gacgaagggc aggtggaaaa
    35701 attaatacag tggttaaaaa gtggcggccc gcgttcagcg cgtgtggaac gggtgcttag
    35761 cgagccgcat catccctccg gggagttaac ggattttcgt atccgttaaa tgcattttac
    35821 cggtttaggc aggccagcaa ttttggtggc ttgctttgcc ggacctttcg gaaacagtcg
    35881 gtataaatag cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag
    35941 catacgaatc gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac
    36001 ttcccagtgt tctggcgaca gcgcaatccc ttcgttctct gcaatcacca ctgccagtgg
    36061 ttcactccac tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc
    36121 ttcgaagatc agcataattc tttactacct taatcagact ggcggcagtg taacaaaaaa
    36181 caaagccccg cataaagcga ggctcggtaa gtaaccctgg gcgagattaa tcgcggctgg
    36241 cgaagcccag aatgctcagc aggctgacga agatgttgta cagcgaaaca tacaggctaa
    36301 ccgtggcacg aatatagttc gtttcaccgc catgaatgat gttgctggtt tcaaacaaaa
    36361 tagcgccaga ggagatcaga atgaagaccg cgctgatcgc cagatgcagg gcaggcagct
    36421 gcaggaagat attagcaacc ataccaatca gcaccaccac aatacccgcc atcagcatac
    36481 cgccaaggaa cgacatatct ttgcgggtgg tcagcacata cgcagagcag cagaagaaca
    36541 ccaacgccgt tccgcccagt gccatagcga ttacgtcacc cattccagca gacaggtagg
    36601 tgttcagaat tggtccgagg atatatccca gaaaaccggt aaaggcgaat gcggagataa
    36661 tcccggtcgg cttattcgcc gttttgtagg tcaggaacat caaaccatac atacccacca
    36721 gcgtcagaat cagacccgga gatggcagca tcagcaccgt actggcagtt gcggtaatcg
    36781 ccgaaaaggc cagcgtcagg ctcagcagaa aataggtatt acgcagcacc ttatgggtgc
    36841 taagcagcga tgtacggtca tgtgaagaac taactatacg atccattgag tcactctcta
    36901 tgacagatgt aattaattaa gcagcataat gataatgtgg gaaggtacac agaagtttta
    36961 cccatcttta cgcatttgat ctggaacagg tttaacagcg gattatcagg tcattaagca
    37021 aatataacgc cctgagaatt tcgacaggca aaagaaaaag gggttagcat ttagctaacc
    37081 ccttatctta tttggcggaa gcgcagagat tcgaactctg gaaccctttc gggtcgccgg
    37141 ttttcaagac cggtgccttc aaccgctcgg ccacacttcc ggaatgacgc gcactataaa
    37201 catcccgatg cggcgtgtaa acccctaatt tgtttgtttg cctgaaaaac agccaaaagt
    37261 gcattgttag cgtgaaataa cagcagactg atcatttcat caccatgaat tccttctctt
    37321 ttactcgttt agcaaccggt taaacatcct caccgcccgg caaaaagaaa aataggtcca
    37381 tttttatcgc taaaagataa atccacacag tttgtattgt tttgtgcaaa agtttcacta
    37441 cgctttatta acaatacttt ctggcgacgt gcgccagtgc agaaggatga gctttcgttt
    37501 tcagcatctc acgtgaagcg atggtttgcc ttgctacagg gacgtcgctt gccgaccatg
    37561 tgcgcccggt gtcctgccgg tgtcgcaagg agaagagacg tgcgatatga ataacgagga
    37621 aacattttac caggccatgc ggcgtcaggg cgttacccgg cgcagctttc tcaaatattg
    37681 tagtctggct gccacgtcgc tgggattagg cgcgggaatg gcaccaaaga ttgcctgggc
    37741 gctggagaat aaaccgcgca ttccggtggt atggatccac ggtctggaat gcacctgctg
    37801 taccgaatct tttatccgct ccgctcaccc actggcgaag gacgtcatcc tttccctgat
    37861 ttccctcgat tacgacgata ctttgatggc tgccgccgga acccaggcgg aagaagtctt
    37921 tgaagacatc atcacgcaat acaatggcaa atatatcctc gccgttgaag gtaatccgcc
    37981 gctgggcgag caggggatgt tctgtatcag cagcggtcga ccgtttattg agaaactcaa
    38041 acgtgccgct gccggagcca gtgcgattat cgcctgggga acctgcgcgt cctggggctg
    38101 cgtgcaggcc gcgcgaccca atccgacgca ggcaacgcct atcgacaaag tcatcaccga
    38161 caaacctatt atcaaagtac ctggctgccc gccgatcccg gatgtgatga gcgccatcat
    38221 tacttacatg gtgacctttg atcgcttgcc agatgtcgac aggatgggcc gtccgttgat
    38281 gttctatggt cagcgtatcc acgataaatg ctatcgccgt gctcacttcg acgccggaga
    38341 gttcgtccag agttgggatg atgacgctgc ccgcaaaggt tactgcctgt acaaaatggg
    38401 ctgcaaaggg cctaccacct ataacgcctg ttcctccaca cgctggaacg atggcgtttc
    38461 cttcccgatc cagtcaggtc acggctgcct gggttgtgcg gaaaatggtt tctgggatcg
    38521 cggttcgttc tacagccgcg tggtcgatat tccacaaatg ggtacccatt ccaccgccga
    38581 caccgtcggt ttaaccgcgc ttggcgtggt ggcagcggct gttggtgtgc acgcagtcgc
    38641 cagcgccgtt gaccagcgca gacgtcataa ccagcaacct acagaaaccg aacatcagcc
    38701 aggcaatgag gataaacagg catgagcact cagtacgaaa ctcagggata caccatcaac
    38761 aatgccggac gccgcctggt ggtcgacccg attacgcgca ttgaaggcca catgcgctgc
    38821 gaagtgaata ttaacgatca gaatgtgatc accaatgccg tctcctgcgg caccatgttt
    38881 cgcgggctgg agatcatcct gcaagggcgt gacccgcgcg atgcgtgggc gttcgttgaa
    38941 cgtatctgcg gcgtctgtac tggcgtacac gccctggctt cggtttacgc catcgaagat
    39001 gccatcggta ttaaagtgcc ggacaacgcc aatatcatcc gcaacattat gctggcaacg
    39061 ctctggtgcc acgatcatct ggtgcacttc tatcagcttg ccgggatgga ctggatcgat
    39121 gtgttagatg cgctgaaagc cgacccgcgg aaaacctccg aactggcgca aagcctctcc
    39181 tcatggccga aatcatcccc tggctatttc ttcgacgtac aaaaccgcct gaagaaattc
    39241 gttgaaggcg ggcagttggg gatcttccgc aatggctact gggggcaccc gcagtacaaa
    39301 ctgccgccag aagccaacct gatgggcttt gcccactatc tcgaagctct cgatttccag
    39361 cgtgaaattg tcaaaatcca cgcggtcttt ggtggtaaaa acccgcatcc aaactggatt
    39421 gtcggaggga tgccttgcgc cattaacatt gacgaaagcg gcgcagtcgg ggcggtcaat
    39481 atggaacgcc tgaacctggt gcagtcgatc attacccgta cggcggattt cattaacaac
    39541 gtgatgatcc ccgacgcttt agccatcggc cagttcaaca aaccgtggag cgaaatcggc
    39601 acgggtcttt ctgataaatg tgtcctcagc tacggcgcat tcccggatat tgccaacgac
    39661 tttggcgaga aaagtctgct gatgcctggc ggcgcggtga ttaacggcga cttcaacaat
    39721 gtgctgccag tggatttggt tgatccgcag caggtgcagg agtttgtcga ccacgcctgg
    39781 tatcgttatc ccaacgatca ggtggggcgt catccgttcg atggcattac cgacccgtgg
    39841 tacaaccccg gcgatgtcaa aggcagcgat accaacattc agcagctgaa tgaacaggaa
    39901 cgctactcgt ggatcaaagc gccacgctgg cgcggtaacg cgatggaagt ggggccgctg
    39961 gcacgcacgt taatcgctta tcacaaaggc gatgctgcga ccgttgagtc ggtcgatcgt
    40021 atgatgtcgg cgttgaaact gccgctttcc ggcatccagt caacgttggg ccgcattttg
    40081 tgtcgcgcgc acgaagcgca gtgggccgca ggtaagttgc agtatttctt cgataaactg
    40141 atgaccaacc tgaaaaacgg caatcttgcc accgcttcca cggaaaaatg ggaaccagca
    40201 acctggccga cagagtgccg tggtgtcggt tttaccgaag cgccgcgcgg ggcgttaggc
    40261 cactgggctg ccattcgcga tggcaagatt gatctctacc agtgcgtggt gccgaccacc
    40321 tggaacgcca gcccgcgcga tcccaaaggg cagattggcg cttatgaagc ggcgctgatg
    40381 aacaccaaaa tggcgatccc cgagcaaccg ctggagatcc tgcgtactct gcacagcttt
    40441 gacccgtgcc tcgcctgttc aacacacgtg ctgggcgacg acggtagcga gcttatctcc
    40501 gtgcaggtgc gttaacagcg aaggagaatc atcatgcaac agaaaagcga caacgttgtc
    40561 agtcactatg tctttgaagc acccgtgcgg atatggcact ggctgacagt gttatgcatg
    40621 gcggtgttga tggtcaccgg ttactttatc ggcaagccgc taccttccgt cagcggcgag
    40681 gcgacgtatc tgttctatat gggctacatc aggttaattc acttcagcgc cgggatgatt
    40741 tttaccgtgg tcttgctgat gcggatctac tgggcttttg ttggcaatcg ttactcccgc
    40801 gagctgttta tcgtgccggt atggcgtaaa agctggtggc agggcgtgtg gtatgaaatc
    40861 cgctggtatc tgtttctggc aaaacgtccg agtgccgata taggccataa ccccatcgcc
    40921 caggcggcga tgttcggcta tttcctgatg tcagttttta tgatcatcac cggctttgcg
    40981 ctgtacagcg aacacagcca gtacgctatt tttgcgccgt tccgttatgt ggtggaattt
    41041 ttctactgga cgggtggcaa ctcaatggac attcacagct ggcatcggct ggggatgtgg
    41101 ctgattggcg cgtttgtgat cggtcatgtc tacatggcgc tgcgtgaaga catcatgtcc
    41161 gacgacacgg tgatctccac tatggtcaac ggctaccgta gccacaaatt tggcaaaata
    41221 agtaacaagg agcgttcatg agcgagcaac gcgtggtggt aatggggctg ggcaacctgc
    41281 tgtgggccga tgaaggcttc ggcgtgcggg tggcggaacg gctgtatgcc cattaccact
    41341 ggccagagga tgtggagatt gtcgatggcg gcacccaagg actgaacttg ctggggtatg
    41401 tcgaaagcgc cagtcatctg ttgattctcg atgccattga ttacgggctg gaacccggaa
    41461 cgctacgaac gtatgccgga gaacgcattc cggcttatct cagcgcgaag aaaatgagcc
    41521 tgcaccagaa cagtttctcc gaagtgctgg cgctggcaga tatccgcgga catctgccag
    41581 cacatattgc cctcgtcggt ctgcaacccg cgatgctcga cgactacggc ggcagcctga
    41641 gcgaactggc gcgggagcaa ctgcccgctg cggaacaggc ggcgctggcg cagctggctg
    41701 cgtggggaat tgtgccgcaa ccggctaatg aatcgcgctg tctcaattat gactgtctgt
    41761 cgatggaaaa ttacgaaggc gttcgcttgc gtcagtacag gatgaggctg gaggaacagg
    41821 gatgagcaac gacacaccat ttaatgcgtt gtggcaacga atgctggcac gtggctggac
    41881 gccggtcagt gaatgccgtc ttgacgactg gcttacgcaa gcgccagacg gcgtggtgtt
    41941 attaagcagc gatccgaaac gcacgccaga agtcagcgat aacccggtaa tgattggcga
    42001 attactgcgc gagtttcccg actatacatg gcaggtagcg attgctgatc ttgagcagag
    42061 cgaagccatc ggcgatcgct ttggcgtctt tcgctttccc gccacattag tgtttaccgg
    42121 cggaaactat cgcggcgtgc tgaatggtat tcacccgtgg gcggaactga taaacctgat
    42181 gcgcgggctt gtcgaaccgc aacaggagcg tgcttcatga gcgaaacttt tttccatctg
    42241 ctggggccag gaacgcaacc gaacgatgac agtttcagca tgaatccact gccgatcacc
    42301 tgtcgggtga atggtgaacc gagtatggcg gccctggagc aatgtgctca cagcccgcag
    42361 gtgattgcgc tgttaaacga gttacaacat caactaagcg aacgccagcc gccgctgggt
    42421 gaggtactgg cagtcgatct gttaaatctc aacgccgacg atcgtcactt tatcaatacg
    42481 cttctcgggg aaggggaagt gtcagtgcgc attcagcagg ctgacgacag tgaaagtgaa
    42541 gttcaggagg cgatcttctg cggattatgg cgggtacgca gacgtcacgg cgaacagttg
    42601 ctggaagaca aactggaggc tggctgcgca ccgctggcgt tgtggcaggc ggcaacgcaa
    42661 aacgtcttgc cgacagattc gctgttaccg ccgcccattg atggcctgat gaatggccta
    42721 ccgttggccc atgaattact ggcacatgta cgtaaccccg acgcgcagcc gcacagcatt
    42781 aatatgacgc aattacccat cagcgaagct gaccggcttt ttctctcacg tctctgtggg
    42841 ccgggaaata ttcagattcg taccattggc tatggcgaga gctatatcaa cgccacgggg
    42901 ttacgccatg tctggcattt acgctgtacg gacaccttaa aaggcccgtt actggaaagt
    42961 tatgaaatct gcccaatacc ggaagtggtg ctggcagcgc cagaagattt ggtcgactct
    43021 gcgcagcggc ttagcgaggt atgccagtgg ctggcggagg gggcaccgac gtaaaaagac
    43081 ggtaagtatc gctttcaggc ttatgaatat cgcaatcggc gaatacctct ggtcgtagag
    43141 tttcaggata aagagggaga tctaccatta acgggttatt tttctctctt cgcctacagg
    43201 agtgcgcatg tgggatgtca ttgatttatc gcgctggcag tttgctctga ccgcgctgta
    43261 tcacttttta tttgtacccc ttaccctggg gctgattttt ttgctggcta tcatggaaac
    43321 catttacgtg gtcaccggca aaacaatcta ccgcgatatg acgcgcttct ggggtaagct
    43381 cttcggtatc aactttgctc ttggcgtggc taccggcctg accatggagt ttcagtttgg
    43441 tactaactgg tcattctatt ccaactatgt gggcgatatt tttggcgcac cgctggcgat
    43501 ggaagcatta atggccttct tcctcgaatc cacctttgtc gggctgttct tcttcggctg
    43561 gcagcggctg aataaatacc agcacctgct ggtgacgtgg ctggtggcgt tcggttcaaa
    43621 tctctctgcg ttgtggatat tgaatgccaa cggttggatg caatacccga ctggtgcgca
    43681 ttttgatatc gacaccctgc gtatggagat gaccagtttc agcgagctgg tctttaaccc
    43741 ggtcagccag gtgaaatttg tgcacaccgt aatggcggga tacgtgaccg gggctatgtt
    43801 tattatggcg atcagcgcct ggtatttact gcgcggacgg gagcgcgatg tcgcattacg
    43861 ctcgtttgcc atcggttccg tcttcggtac tctggcgatt atcggtacgc tgcaactcgg
    43921 agacagttct gcgtatgaag tcgcgcaagt acaaccggta aaactggcgg cgatggaagg
    43981 ggagtggcaa acggaacctg cacctgcacc gttccatgtg gttgcctggc cggaacagga
    44041 tcaagagcgt aacgcctttg ccatcaaaat tcccgcgctg ctaggaatcc tcgccactca
    44101 ttcattagat aaacccgtgc cgggtctgaa gaatttgatg gctgaaacct acccacgctt
    44161 gcaacgcgga cgtatggcgt ggctgttaat gcaggaaata tcgcaaggca atcgtgagcc
    44221 gcatgtgttg caggcattcc gggagctgga aggcgacctg ggttacggca tgttgctctc
    44281 ccgctatgcg ccggatatga atcatgtcac agccgcacag taccaggcgg cgatgcgtgg
    44341 cgcgatacct caggttgcgc cggtattctg gagtttccgc atcatggtgg gctgtggttc
    44401 cctgctgtta ctggtgatgc tgattgcgct tgtccagacg ctgcgtggca aaatcgacca
    44461 gcatcgctgg gtgctgaaaa tggcgctctg gagtttgcca ctgccgtgga ttgcgattga
    44521 agccggatgg tttatgaccg agtttggtcg tcagccgtgg gcgatacagg acatcttacc
    44581 gacatactcc gcgcactccg ccttaaccac aggacaactg gctttctcac tgatcatgat
    44641 cgtagggctt tacaccctgt tcttaatcgc cgaagtctac ctgatgcaga aatatgcccg
    44701 tctggggccg agcgcgatgc agagtgaaca accgacgcag caacaggggt aaaggagaaa
    44761 atcatgtttg attatgaaac attgcgcttc atctggtggc tgctgattgg cgtgatcctg
    44821 gtggtcttta tgatctccga cggatttgac atggggatcg gctgtctgct gccgctggtg
    44881 gcgcgtaatg atgatgaacg tcggatagtg ataaacagcg ttggcgcgca ctgggaaggc
    44941 aatcaggtct ggttgatcct cgcaggtggg gcattatttg ccgcctggcc cagagtgtat
    45001 gcagcggcgt tttccggctt ttatgtggcg atgattctgg tgctgtgctc gctgttcttc
    45061 cgcccgctgg cctttgatta tcgcggaaaa atcgccgatg cacgctggcg taaaatgtgg
    45121 gatgccggac tggtcatcgg cagtctggtg ccgccggtag tcttcggtat tgcattcggc
    45181 aacttgctgc tcggcgtgcc gtttgctttc acaccgcaat tacgcgtgga atatctcggc
    45241 agcttctggc aactgctgac gccattccct ttattgtgtg gattgctcag tcttgggatg
    45301 gtgattttgc aaggtggcgt ctggttacaa ctgaaaactg ttggtgtgat tcatctgcgt
    45361 tcacagctgg cgaccaaacg cgccgcacta ctggtgatgc tgtgcttttt gctggcgggt
    45421 tactggctgt gggttggtat tgatggcttt gtactgctcg cccaggatgc taacggtcct
    45481 tccaatccgt taatgaaact ggtggcagtg ctacctggtg cctggatgaa taatttcgtc
    45541 gaatcgcccg ttttgtggat cttcccgctg ctggggttct tctgcccatt gctgacggtg
    45601 atggcgattt atcgtggtcg cccgggttgg ggatttttga tggcgtcatt gatgcaattt
    45661 ggcgtgattt tcacggcagg catcacgctg ttcccctttg tcatgccgtc aagcgtgagt
    45721 ccgatctcca gcctgacgtt atgggacagc acttccagtc agctgacgct gagcattatg
    45781 ttggtaatcg tgctgatatt tttgcccatt gtgttgctct acactctctg gagctactac
    45841 aaaatgtggg ggcgcatgac gacagaaact ctccgccgta acgaaaacga gctgtactaa
    45901 ggagcaggaa caatgtggta tttactttgg ttcgtcggca ttttgttgat gtgttcgctc
    45961 tccacccttg tgttggtatg gctggacccg cgtctgaaaa gttaacgaag gtaagcctga
    46021 tgaggcgcat tagcgtcgca tcaggcaatc aataatgtcg gatatgaaaa gcggaaacat
    46081 atcgatgaaa gcgatcttga tcccattctt atctcttctg attccgttaa caccgaaatc
    46141 tgcattcgct cagagtgagc cggagctgaa gctggaaagc gtggtgattg tcagtcgtca
    46201 cggtgtgcgt gcaccaacca aggccacgca actgatgcag gatgtcaccc cagacgcatg
    46261 gccaacctgg ccggtaaaac tgggttggct gacaccgcgc ggtggtgagt taatcgctta
    46321 tctcggacat taccaacgcc agcgtctggt ggccgacgga ttgctggcaa aagagggctg
    46381 cccgcagcca ggtcaggtcg cggttattgc tgatgtcgac gaacgtaccc gtaaaacagg
    46441 cgaagccttc gccgccgggc tggcacctga ctgtgtaata accgtacata cccaggcaga
    46501 tacgtccagt cccgatccgt tatttaatcc tctaaaaact ggcgtttgcc aactggataa
    46561 cgcgaacgtg actgacgcga tcctcagtag ggcaggaggg gcaattgctg actttgccgg
    46621 gcatcggcaa acggcgtttc gcgaactgga acgggtgctt aatttcccgc aatcaaactt
    46681 gtgccttaac cgtaagaaac aggacgaaag ctgttcatta acgcaggcat taccatcgga
    46741 actcaaggtg agcgccgaca atgtctcatt aaccggtgcg gtaagcctcg catcaatgct
    46801 gacggagata tttctcctgc aacaagcaca gggaatgccg gagccggggt ggggaaggat
    46861 caccgattca caccagtgga acaccttgct aagtctacat aacgcgcaat tttatttact
    46921 acaacgcaca ccagaggttg cccgcagtcg cgccaccccg ttattggatt tgatcatgac
    46981 agcgttggcg ccccatccac cgcaaaaaca ggtgtatggt gtgacattac ccacttcagt
    47041 gctgtttatc gccggacacg atactaatct ggcaaatctc ggcggcgcac tggagctcaa
    47101 ctggacgctt ccaggtcagc cggataacac gccgccaggt ggtgaactag tgtttgaacg
    47161 ctggcgtcgg ctcagcgata acagccagtg gattcaggtt tcgctggtct tccagacttt
    47221 acagcagatg cgtgataaaa cgccgctatc attaaatacg ccgcccggag aggtgaaact
    47281 gaccctggca ggatgtgaag agcgaaatgc gcagggcatg tgttcgttgg ccggttttac
    47341 gcaaatcgtg aatgaagcac gcataccagc gtgcgctctc catcaagaca aataaacatt
    47401 tgccgctgtt ggtccacgca ggccatttac gcggcaaaat tccacgcgta atcctggtat
    47461 aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg acctggactt ctttacgacc
    47521 gtcggagggg ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt
    47581 cattttacgg gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa
    47641 taaaataaag ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct
    47701 caattatcat gcgttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt
    47761 actaaagcag ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac
    47821 taagtagttt ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat
    47881 atgtctaata aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt
    47941 atcactcctg atgatggcag caaagacgtt ttcgtccatt tcactgccat ccagagcaat
    48001 gaattccgca cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt
    48061 ggtcccgcgg cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca
    48121 tttccgctgt ccatgttgcc atggttcaca gtaccgcaca tcggcattcg atgtgacgga
    48181 gcgaaatcct ttaggagcaa agtgcatttt ttgtaaatcg acgatgatca cctttgataa
    48241 cgtcgcgctg caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatctggc
    48301 tccctcgtgg agcctttttt atatctgcct tatttttctt caatgctgta tgtatagtaa
    48361 gcgataacct gttgattatt gaatctttcg gggagatggc ttataacatt tcttacctga
    48421 ccagggtacc gggaaccaac accttactgg cgtgttgctg tcttttaaga ccagaagagg
    48481 ttaacagtga atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc
    48541 gcgcaaaaaa tcgccgagct gaacaagaat tcagggaaag aagtctctga aattcgcttc
    48601 accgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga
    48661 ttttgtgaac atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat
    48721 cattggcgat tgcggaatta agcgttggta caattcctcc ggcaccgggc tttgccatac
    48781 tcccgcatac attgcgtaac caatcgccgc aaacataatc cccataacca gtagcgtcat
    48841 taaccagcca gacaacgcaa aggctttttt atttgccgca ggtttttgca gtgaaaaggt
    48901 caatgttgag gctaccggac atgactctac gcaagtcata cagccggtac attccactgt
    48961 tcgtacctga attaatttat cgaccgggat ccgtgatggg caactttttg cgcatttgcc
    49021 acaatcgata caactttcgg cattgcgacg aatcttaaac ggcgataata gcgaaaccac
    49081 gcccatcagc gcgccatatg ggcaaagata acgacaccag acatggcgaa taaacaggct
    49141 ggcaatcagc aaaacgatca tgctgattaa tgtcgcggtc cccatatgac gaaagaaatc
    49201 gagcatttta acgtccatca ccacgctgta gggcgacaac ataaaatagt gaatcgcctg
    49261 agcgggcatc aataacgcga tatagagaaa aaaactcaac agcaaatact tcacgccgcg
    49321 cagaggaata tccagccagc ggggaaggac aaattgccga ccaaacagtt tgttaccgag
    49381 atcgccgatt aattccgaaa gcgtaccaac cgggcataac catgaacaaa aggccttttt
    49441 gagtaataga ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga
    49501 cggtaactga cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca
    49561 gccttcgata cctcccggtc tggtgataaa tgtcgtacta cctgccgttt cgtaatagcg
    49621 cacccaatac cagaatgtga tggcaatata aatattaatt gccaacagta ataattgcgt
    49681 cgctttacgc caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt
    49741 gcctggccgc cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta
    49801 agttgcatac caaatgccac tattctagtt gttcttaact ggctgatatt gattcaaatc
    49861 gcgttcaggt ttttcttatt aaccatgctt ccagagcggc aacactgcgt gtaatttctt
    49921 cgtgtggaag gggggcagat aatggctgct gctccagttg tgcgcattgc tggctggcgc
    49981 tacgcattcc cagactcgag caactgcttt ttagctgatg tgcggcacgc tttattttct
    50041 cgttatcctg actggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt
    50101 taaataatat cagccattcg tggatcttct ccgcccccat taaatgagca tcttcattga
    50161 gttgcgatac atccagcggt tgatcatttt tgacttgcag ttggagatag tgcgccagta
    50221 attgaccgag cacttcacgc ggcaccggtt tagggataat cccgcgaaat agcgaactgg
    50281 tacgctggcg cagtgtttcg tcgatgacat gagcgctaaa gccaatcaaa accagcgacg
    50341 gatattgctg tgccagttgc cgggcaagcg taatgccgtc gatatccggc agatcaaaat
    50401 ccaccagtgc gacagcaaat ggttcactat tttgcagtgt ctctaaagcc tgcgcggcat
    50461 ttcctacggc aacgacctgc gccccactgc tgttcaacat ctcaacggta atccgctggg
    50521 ttagcgggtt atcttcaatt aacagcaaac gtaaaccgtc cagacgcacc gcctgattga
    50581 ctgtttttgg cacgggtgca gtggcaatac gtaacggcaa gcgtaaacaa aaacagcttc
    50641 caacctccgg cgtgctggtg gcgctcagtt cgccacccat cgcctgggcc agacggctac
    50701 tgatagtcag ccccagcccg gtgccgccgc gtttgccgct aacctgaaca aatggctgga
    50761 agatttcagc cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc
    50821 attgttcgcc atcagtgcga ctacgcagaa taatgtgccc ttcgtcggtg aaacgcaggg
    50881 cgttgctcaa caggttggta atgacctgac gaatacgccg cggatcgccc attaacgcag
    50941 tcggtacatc gtcggcaatt tctgttgcca ggcgaatcgg gcgacctttc acccttccgc
    51001 tcattaattg cagggtactt tccagcagcg ggcgcggttc aaagggttca tcgctgaccg
    51061 aaacattctt gccgcctgct tcgatagccg aataatcgag aatatcgttg aggatggtca
    51121 gcaacgattc accagagtcg gtaatcgccc gcaaatcatc gcgctgggcg ttaagtgcgg
    51181 ggttatctgc cagcagttgc gcggtgccga gaataccgta cagcggtgtg cggatctcat
    51241 ggctcatcgc cgccagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg
    51301 cctgtcggtg ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg
    51361 ccagctgttc gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc
    51421 gcccgatggt atccagttcc cgtacgccag cggtttccgg gaaaggggag tcgatatcac
    51481 catccagcaa gcgttgcagc gcctgcgttt gttctgcaag agggcgcgtg actgagcgat
    51541 aaaccacgcg ccagaggatc agaatcagtg cgcaaagaga aactaatccc agcaataaca
    51601 ggctgtattg cccgcgtgca ctggcttttt ccagatgtgc cagcccgtgc tgattacgca
    51661 gctcaatggt gtcgaccagc tgactgactt cgctactaaa ctgcgcgaat tgggcgatgt
    51721 tattttgggc gagagtttgc aggtgattgc tgatttcact gtcctgctga tacagcgcca
    51781 gcaaatcgct atattggcta acggtagtta acgttgttgc tacctgagta cgaacacccg
    51841 gatcttcaat gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt
    51901 ccagcgttgg cgcatttttc tggatctgtt ccagccccag attcatcacc atttgctgca
    51961 cccgcagggc gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa
    52021 tcagccgatc gagtgcactt tctgcggcct gacgctgatg ttgttcgatc aaatcgtaaa
    52081 tcccggcctg ggtcgctccg gcggaggttg ccgcattatt cgcctgacct tgcgccaggc
    52141 gtgcgatttc atcggcggcg gcgactatct gctgactgag ttgctgttgt tgctggcgaa
    52201 gttgcaaacg ctgccccacc agttcccctt gctgacgtaa cgaacgggag atctcctgct
    52261 cctgttgttc aatagcggtg gtgtcaaaac cttgttcccg taacgcttgc agcaacgcat
    52321 taatcttcag gctttgtgcg gtgagcattc gcccctgcgc ctgccacatc ttttcgttat
    52381 cggcactggt caggttctgc gcggcgaaaa gttcccaggc gctggcttcg ctcaactggc
    52441 gcgccatatt catggtagga atcaatgcct gagtgttgtc tttttccacc tggctgataa
    52501 agcgcaggtt gtaccatccc accagggtac tggtcagggt taacagcgcc atcagggcaa
    52561 agcccatcca gagtcttcgg gtcagggtta aattcacggt cggtgcactt taggtgaaaa
    52621 aggttgagtc gcaaagcaga atgcatctag cataaagcct tattattgat gaggctatca
    52681 tgcgcgtact gctattttta cttctttccc ttttcatgtt atcggcattt tcggctgata
    52741 acctgttgcg ctggcatgat gcgcagcatt acacggtgca agcctctacg ccgcttaaag
    52801 ccaaacgcgc atggaaactg tgcgcgcttt atcccagcct gaaagattca tactggttat
    52861 cgttgaacta tggtatgcag gaggctgctc gccgctacgg tgtggattta aaagtgctgg
    52921 aggcaggcgg ctacagccag ttggctacgc agcaagcaca aatcgaccag tgtaaacagt
    52981 ggggcgcaga ggccattttg ctcggtagta gcacgacgtc atttcccgat ctgcaaaagc
    53041 aggtagcaaa tctgccggtg atcgaactgg tgaatgctat tgatgctccc caggtgaaaa
    53101 gccgcgttgg tgtgccctgg tttcagatgg gctatcaacc agggcgatat ctggtgcaat
    53161 gggcgcacgg taaaccactg aacgtgctgt tgatgcccgg ccccgataac gccgggggca
    53221 gtaaggagat ggtagagggt tttcgcgcag ccattgccgg aagcccggta cgtattgttg
    53281 atattgcgct cggtgataac gatattgaaa tccagcgtaa cctgctgcag gagatgctgg
    53341 aacgccatcc agaaatcgac gtcgttgccg gaacggccat tgcggcagag gcggcaatgg
    53401 gggaagggcg caacctgaaa acgccgctta ccgtggtgtc gttttatctt tcacatcagg
    53461 tgtatcgcgg gttgaagcgg ggaagagtga ttatggcagc cagcgatcaa atggtctggc
    53521 agggggaact ggcggttgag caggctatca ggcaattaca ggggcaatcg gtgtctgata
    53581 atgtcagccc accgatttta gtcatgacgc cgaaaaatgc cgaccgcgaa catatccgcc
    53641 gctcgctgtc accaggggga tttcgtccgg tttattttta tcagcacaca tcagcggcta
    53701 agaaataacc ttcgccatgt tgtgtcacca gtaaatccgc actgagttta tggcgtaaac
    53761 gacgaattaa cacatcgacg gtgcgcaggt cagggttttc cacccgacgc gcagagagca
    53821 tacgcagcag acgttcacgg ctgagaattt cgcccggatt agtcacaaat gccaccagca
    53881 tttcatactc tgcgcgggtc agtttaatcg gctcgccatc ccgctccagc gtatggcgcg
    53941 acacattcag gcaataaccg gcaaagcgat agcagttgtc ctgagtgtgc ggttgcgcct
    54001 gtcgcgcgag gtcgattcgc cagagcaaat ttttcactcg taccaccagt tcgcgcagct
    54061 ccagcggttt ggtgacgtaa tcgtctgcgc ccatttccag cccaacaata cggtcaatcc
    54121 gatcgctgcg tccggtaacc agaataatcc ccaccgttga gcgttctcgc agggcgcggg
    54181 ttaacatcag gccattttca tcgggcaaat tgatatccag cagaatcaaa tccaccggct
    54241 gattctgcat aatttcccgc agcccggcac cgctcgccgt aacggaaacg gtatacccct
    54301 cctgagtgaa gtaagattgt aatcgcgcct gggtaaccgg ctcatcttca acaataacaa
    54361 tgtgatgtgg catcagaggg ttttactcat tctgttcata tctgttcata ttctgccgta
    54421 agccgttcat cctgaccagt gccgctgttc atatttgctc attaagatcg cttcactaaa
    54481 ccataatttt acaggggcta ttatgcggaa actctggaac gcgctacgcc gacccagtgc
    54541 tcgttggtcg gtactggcgc tggtcgctac tgggattgtg attggcattg cgctgattgt
    54601 attgccacac gttgggatca aagtcaccag cacaaccgaa ttttgtgtca gttgccacag
    54661 tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat ttccagaacg cctccggcgt
    54721 gcgagctgaa tgccatgact gtcatatccc gccggatatt ccaggcatgg tgaagcgcaa
    54781 actggaagcg agcaacgaca tttaccagac ctttattgcc cactccattg atacacctga
    54841 gaaatttgaa gccaaacgcg cggaacttgc cgagcgtgaa tgggcgcgaa tgaaagaaaa
    54901 caactcggca acctgccgct cctgccataa ctacgatgcg atggatcatg cgaagcagca
    54961 tcctgaagcg gcgcgtcaga tgaaggtggc agcgaaagat aatcaatcct gcatcgactg
    55021 tcataaaggt attgcccacc agttaccgga tatgagtagt ggcttccgta agcagttcga
    55081 tgagctgcgc gccagtgcta atgatagtgg tgacacgctg tactccattg atattaagcc
    55141 gatttatgcg gcgaaaggcg ataaagaagc gtctggttct ctgctgcctg cttcggaagt
    55201 gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa atcactggct ggacggaaag
    55261 tgcaggacgt cagcgtgtac tcacccaatt cccaggtaaa cgcatctttg ttgcctcgat
    55321 tcgtggtgat gtgcagcagc aggtgaaaac actggagaaa accaccgttg ccgacaccaa
    55381 taccgagtgg agcaagttgc aggccactgc gtggatgaag aaaggcgaca tggtgaacga
    55441 tatcaaaccg atctgggctt atgcggattc gctgtacaac ggcacctgta accagtgcca
    55501 cggcgcaccg gaaatatccc actttgacgc taacggttgg atcggcacgc tcaacggcat
    55561 gattggcttt accagcctcg ataaacgtga agaacgcacc ttgttgaaat atctgcaaat
    55621 gaatgcgtct gacaccgcag gtaaagctca cggcgataag aaggaagaaa aataatgaac
    55681 aataacgatc tctttcaggc atcacgtcgg cgttttctgg cacaactcgg cggcttaacc
    55741 gtcgccggga tgctggggcc gtcattgtta acgccgcgcc gtgcgactgc ggcgcaagcg
    55801 gcgactgagg ctgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
    55861 cgcgcgacgg tgaaggatgg tcgctttgtg gcagcaaaac cgttcgaact ggataaatat
    55921 ccgtcgaaaa tgatcgccgg attgccggat catgtacaca acgcggcgcg tattcgttac
    55981 ccgatggtac gcgtggactg gctgcgtaag cgccatctga gcgacacctc ccagcgcggt
    56041 gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg acatgttcta tgaagaactg
    56101 gaacgcgtac agaaaactca cgggccgagt gccttgctga ccgccagtgg ttggcaatcg
    56161 acggggatgt tccataacgc ttcggggatg ctggcgaaag ctattgcctt gcatggtaat
    56221 agcgttggta cgggcggaga ttactctacc ggtgctgcgc aggtgatctt gccgcgcgtg
    56281 gttggctcaa tggaagtgta tgaacagcaa acctcctggc cgctggtatt gcagaacagc
    56341 aaaaccattg tgctgtgggg ctccgatttg ctgaaaaacc agcaagcgaa ctggtggtgc
    56401 ccggatcacg atgtttatga atattacgag cagctaaaag cgaaagtcgc cgccggtgaa
    56461 attgaggtca tcagcatcga tcctgttgtc acatccaccc atgagtatct ggggcgcgag
    56521 catgtgaagc acattgcggt taacccgcaa actgacgtgc cgctgcaact ggcgctggcg
    56581 tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc tcgctaacta ctgtgtaggt
    56641 tttgagcagt tcctgccgta tctgctgggt gagaaagacg gtcagccgaa agatgccgca
    56701 tgggctgaaa aactgaccgg cattgatgcc gaaaccattc gtgggctggc acggcagatg
    56761 gcggcgaaca gaacgcagat tattgctggc tggtgcgtac agcgtatgca gcacggtgaa
    56821 cagtgggcgt ggatgattgt cgttctggcg gcgatgctgg ggcaaattgg cctgccaggt
    56881 ggtggttttg gttttggctg gcactataac ggcgcaggca cgccggggcg taaaggcgtt
    56941 attttgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgactac
    57001 aaaggctaca gcagcaccat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
    57061 aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt
    57121 gccggaacta acccgttcca tcgccatcag cagatcaacc gcattattga aggctggcgc
    57181 aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat
    57241 atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
    57301 tccaatcgtg gcatcatcgc catgaaacag gtcgtgccgc cgcagttcga ggcgcgcaac
    57361 gacttcgata ttttccgcga gctgtgccgt cgctttaatc gcgaagaagc ctttaccgaa
    57421 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acagggcaaa
    57481 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtatgtcgag
    57541 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
    57601 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc tgatatgaac
    57661 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
    57721 cctggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
    57781 tcgcagttat gtgagtcgga aactctgcgt cagcaatata ccgtagcggg taaagagcca
    57841 gtatttatta gcccgcagga tgccagcgcg cgcggtattc gtcacggtga tgtggtacgc
    57901 gtctttaacg ttcgtggtca ggtgttggct ggagcagtgg tttctgaccg ttatgcaccc
    57961 ggcgtggcgc gaattcacga aggggcatgg tacgatccgg acaaaggcgg cgagccaggg
    58021 gcgctgtgca aatacggtaa ccccaacgtc ttgaccatcg acatcggtac ttcgcagctg
    58081 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa cggaacagtg
    58141 gagcaggtga cggcgtttaa cggccccgtg gagatggtgg cgcagtgcga atatattccc
    58201 gcgtcgcagg tgaaatcatg accacgctga cagtacaaca gattgcctgt gtttacgcct
    58261 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
    58321 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc tccgctcact gcggcggtga
    58381 atgggctgga aaacagtatt gccactctga cagtacgtga cgatgcccgt ctggaactgg
    58441 ccgctgactt ttgcggcctg tttctgatga ccgacaaaca agcggcgctg ccgtatgcat
    58501 cggcctacaa acaggacgag caagagatta aacgcttgtt agttgaggca gggatggaga
    58561 ccagcggcaa tttcaacgaa ccggcagatc atctggcgat ctatctcgag ttgttgagtc
    58621 atctgcattt ttcgctggga gaggggaccg ttcctgcgcg aagaatcgac ggtttgcggc
    58681 aaaaaacact gacggcgctg cgggaatggt taccagagtt tgctgcgcgt tgtcgacagt
    58741 atgacagctt tggtttttac gcggcactaa gccagttatt gctggtgtta gtggagtgtg
    58801 actaccaaaa aagataatgc cgtttgtagg cctgataaga cgcggcaggc gtcgcatcag
    58861 gcattagtgc gcagttgccg gatgcggcga gaacgcctta tccggcctac gggaacagtg
    58921 ctttttgtgg gcctgataag atgcgttaag cgtcgcatca ggcattatgg cgcagttgcc
    58981 ggatgcggcg agaacgcctt atccggccta cgggaacagc gctttttgtg ggcctgataa
    59041 gacgcgttaa gcgtcgcatc aggcaacaaa ctcacgggtg agccacaaac cgggaaagcc
    59101 gctggcgcag caggcggttt tcctgcttca ggtgcgcaat atcatccatt aacgtcagcg
    59161 ccaccgcgat ccccggccag tccagagcca gttcatgacg caggcgtacc gcgcgttgca
    59221 ccacaatggc ggcatggtcg tcaaataccc aggttgtctc ctggatctca ctcggttcaa
    59281 ccacccccaa accgacaatt tcattcaact cctcttcaga gatgccggta tgcaggcaaa
    59341 attcggtaat agtaaaagtc accgtaacat tagccattat gctttccccc aatctttacg
    59401 tggatcaaaa gacgactggg cgtctgccag ttgctgccac agcgcggcag tgttttcatc
    59461 cggtttcggc ggcatcacga ttttcagtac cgcatacaga tcgccggtct gttttttgct
    59521 caccagacct ttgcctttaa cgcgcaatcg ttgcccggcc tggctgccag gcgggatagt
    59581 cagcaaaatg ctttctttca gtgttggaac ggtgacttta gtacccagcg ccgcttccca
    59641 cgggctaacc ggcaccacta tttccagatc ctggccgaca atatcaaaca gcggatgtgg
    59701 cgcaatgtga atcaccagcc acaggtcgcc atttggaccg ccgttttcgc ccggcgtccc
    59761 ctggcctttc aggcggatgc gttgaccatt gccgacgccc gccgggatct tcacattcag
    59821 cgttttcggg atttcctgtt cgatcatgcc aaaggcgtta taaaccggca ggttatagct
    59881 gatggtacgc ttatgctcag taagcgtttc ttcgaggaat accgccactt cgatttcaat
    59941 atcgtggccg cgtgtggcgg ggcgttgacg gctctggcgg gcatgctgac cgaaaattga
    60001 cgagaagata tcgtcaaaat cttcggcgtt aaaactctga ccgtcgccat gatggaactg
    60061 acggttaaat tgcggatcgt tgcgatgttg ccacatctga tcatactcag cgcgacgttg
    60121 ttcatcactt aacacttccc aggcttcagc gacctctttg aagcgggctt cggcatccgg
    60181 ttctttgctg acatcaggat ggtatttgcg ggccagtcga cgataggcgg tcttgattgt
    60241 cttgagatcg tccgtcggtt tcacgcccat gatggcgtaa taatccttta attccatagc
    60301 gttatctcgc gtaaatcaac acaaattgaa ggaacccctg taaggtaact cctataagtg
    60361 tagggtaatc ctcaaaattt catatgccaa cacagaatat gttattgaaa tcatcgcgga
    60421 ggggaggtcg cctcaaggtg ggaggaacat attttattta cctcactcct gaacttttgg
    60481 ctcatgctta caagttaaac aataaggaaa ggaatgcaca actcgactat ttaaatcgtt
    60541 atgcaattta atgataaaga catgtgctaa aaaccattgc tattagtctc acattctttt
    60601 tttattggta atggtaacgc cgctgatttt ctttatagct actgatgtaa tcattagcgg
    60661 cgtaaacgag gccggggcta atcaggcata gtttgcgtca aaacttgcct gtttttaaag
    60721 gggaatatag aaaaacaggc gttcaacgag ccattttgcg aacctgttcc cggaaaaatg
    60781 acatatttct gtcacactct ttaattattg ataacaaaag aggtgtgagg aatgaacaaa
    60841 acgctaatcg ccgcaactgt ggcagggata gttttactcg cttcaaatgc tcaggcacaa
    60901 accgtaccgg aaggctatca gctacagcaa gtccttatga tgagccgtca taacttacgt
    60961 gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcga cgccgaataa atggcctgaa
    61021 tgggacgtcc ccggtgggca gctcaccact aaagggggcg tgctcgaagt gtatatgggc
    61081 cattatatgc gtgaatggct ggcagaacag gggatggtga aatcagggga atgcccgcca
    61141 ccggacaccg tttatgccta tgccaatagt ctgcaacgca ccgtcgcgac cgcgcagttc
    61201 tttattactg gcgcattccc tgggtgtgat attcatgttc atcaccagga aaaaatgggc
    61261 actatggacc ccacctttaa cccagtgatc accgatgatt ccgccgcatt cagtgaacag
    61321 gcggtggcgg caatggagaa agagctcagc aaactccagc ttaccgacag ctacaagcta
    61381 ctggaacaaa tcgttaacta taaagattcc cctgcctgta aagagaagca acaatgttcg
    61441 cttgtagatg gcaaaaatac ctttagcgcg aagtatcaac aagaaccggg cgtttccggg
    61501 ccgctgaaag ttggcaactc gctggtagat gcgtttactt tgcaatacta cgaaggtttt
    61561 ccgatggatc aggtggcctg gggagaaatt aaatctgacc agcagtggaa ggtgttgtcg
    61621 aagctgaaaa acggttacca ggacagcctg tttacctcac cggaagtggc gcgcaatgtt
    61681 gcgaaaccgc tggtcagtta tatcgacaaa gccctggtca ccgatcgcac cagtgcaccg
    61741 aaaatcacgg tattagttgg gcacgactcc aacattgcct ctctgttaac ggcgctggat
    61801 ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc cgattggcgg caaaatcgtt
    61861 ttccagcgtt ggcatgacag caaagccaat cgcgacctga tgaaaattga atatgtgtac
    61921 cagagtgcgg aacagttacg taatgccgat gcgttaaccc tgcaggcacc tgcgcagcgc
    61981 gtgacgctgg aattaagcgg ttgcccgata gatgccaacg gtttttgccc aatggataag
    62041 tttgatagcg tattgaatga agcggtgaaa taacagaaaa ctcccccgcg tgaagcgggg
    62101 gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc gccccaaaaa agcgaatctt
    62161 tatcggtctt agcaaaggct ttgactaaca cttcatcgct accttcttcc caaatctttt
    62221 ccgccatttt ttcgtcgtac ccggcgactt cgaaaatggc ctcggctatt tccggcgacg
    62281 tattgcgcag agatgcccat tcaccgacgt gatgagcttt cgcttcttga gttggcatgc
    62341 gtatcctcct gttgaagatt agccgttaag tttaactgcc agacctgcga catattcccc
    62401 ttgataacga gcaatagaca gttcttcctg gctgggctgg cgtgaaccgt caccgcctgc
    62461 gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt gaaacgtcaa ataattcctg
    62521 cgctgcgtag ccaatgggga caatcaccat gccgtgatgc gcaagggtcg tccaggtgga
    62581 tgtgatggtt tgttcctgac cgccgccagt accggtggaa ctaaagacgc tcgccagttt
    62641 tccgtatagt gcgccggaag cccacaggcc gcccgtctgg tcgaggaagg tacgcatttg
    62701 accggacatg ttaccaaagc gggtaggtgt accaaaaata atggcgtcgt aatcggccag
    62761 ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg cctgcttttt caaataattg
    62821 cggcggcatg gtttccggta cacgcttaac gacaacttct gcgccatcca ctttgcttgc
    62881 accctcagca actgcgcgtg ccatcgtttc aatatgtccg tacatggaat aataaagcac
    62941 cagaacttta gccatttcta accactcctc gtgttatctc tattccgtag cgattcgcta
    63001 ccacttattt aaagataaga cgtccttttc agagtgcaaa tttcacaacc acttatttga
    63061 tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa tgacacattt ttatctcatc
    63121 gcgttttttt aatcataaga gcggcttatg gataattatt ggagatgata tctattctcg
    63181 ctaagaagct gttgcaggat attaccaaac gcgggtctgc ccgcgtcagt tcactaagct
    63241 tagtcccacg tagcgaaaat atggcagccg ccatacgccg cgttaattct atgcaatatg
    63301 atgtctatac ccagacggag gtcagtaatg gcaaaccatc gaggcggttc cggcaatttt
    63361 gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag gtggacagca cagcgggggt
    63421 aatttcaaaa atgacccgca gcgcgcatct gaagcaggta aaaaaggtgg caagagcagt
    63481 cacggtaaaa gcgacaacta gccgggctaa tcaatgacgc atgcattttt gtctgtagct
    63541 cgtcaaaaag ccatcaccgc cggttacccc ggcggttttc tgtttatggc ttctgatgaa
    63601 caacttctgg cggtggaacg tcaaccaacc ttctgcttaa caacgcattg agtaaaatcg
    63661 cgccaaaagt tgctgtaccg atccctccca acgtaaaacc gcccagcgtg agagcaaaat
    63721 cacccgcacc cagcactaag gttactgcga ccataatcaa attaccgttc tggcttaaat
    63781 ccacactgtt ttgtacccag atccgtgcgc ctgcgacggc aatcagcccg aacacaacaa
    63841 ttgatgcacc accaatcacc gcggccggaa tagtatggat cagcgcgcca aatttcggcg
    63901 aaaagcccaa cagcatggcg atgacggcag cggcaacaaa caccagcgtc gagtagactt
    63961 ttgtcacggc catcacacca atattctcgg cgtaggtggt tacgccgcta ccgccgacag
    64021 agccagaaag catcgtcgcc agcccatcgc ctacgaatgc ccgccccata tacgggtcca
    64081 tattgcgtcc ggtcatcccg gcgaccgcct tgagatgacc taagttttcc gccaccagaa
    64141 tcaccgccac gggcgcaatc agcatcattg cctgaccatt aaaagcagga gtggaaaaat
    64201 gtggcagacc gaaccaggca gcatggctga cgagagtaaa atcgacggct tttcccagcc
    64261 ctaaaacgtt ggtcatcacg ccatccagct atcaaaggcc gaagccgata catttttcac
    64321 tgcgataggc gctaagttca ggccaatcgc catcactacc gcacccgtca caaccggcgg
    64381 cattagtcgt tcaatccagc gcgtaccgat tttcatcacc accaggccaa tgacggtata
    64441 aaccagccca caggcgataa tcccgcccag cgcgatgctg atattcggat taattccctg
    64501 accattaaag cccgtcgcgg caatcaccac gccgacaaaa gccgcgctgg aaccgagata
    64561 actgggcacg cgcccgccgg taataaagaa aaacagtaac gtgccgatcc ccgacattaa
    64621 aatggaaaga ttgggatcca gccccatcag aattggcatt aacaccgtcg cgccaaacat
    64681 cgccaccgcg tgttgaacgc ccattactgc cgtttgagca aacggcaatc gttcatccgg
    64741 cgcgaccacg ccgctctctg tagaggtcga ttttaactgc cagtgaggaa aaccgaacat
    64801 tgccatcagc tgtctcctta aggaggttaa caagcagggc gcatcagcgc gtgataactg
    64861 cgatcgaacc acaccagccc gtagggtgtg gcgtgacgat gaatcgcttc gatggcgcaa
    64921 aacaaaatgt cgtgggtgcc gacgctcacc acctggctga tacggcagtc aaacgaaacc
    64981 agtgcctctt ccagttgcgg gcatccggtc acaccagtct gccagcgggc ggcggcaaag
    65041 cggagttcca tgggcgtttt gccgccaaaa aggtttgaaa gcggctcctg cccggcgcta
    65101 agtgtattta cacacagcgt tcgattttca ttgaataccg gccagacgga cgccccacga
    65161 ttcaggcaca ccagtaatgt gggcggcgtg tcagtcacgc tgcagacggc gctggcggtg
    65221 aacccggcgc gcccggctgg accgtcagta gtgatgatat tgaccgccgc gcccatacag
    65281 gacatcgcat cgcgaaaggt ttgttgatcg acaatgttca ttgtttgctc cttacaacag
    65341 cccgcaggct tcttcaaagg acagacgtgg caggcgcgca aaaagtttgc tggaatcgcc
    65401 atagccgata ttaatcagca gattgctctt cagcgtgctg cccgcaaaca aggcgtcgtc
    65461 aacgtattga cggtcaaagc ccgacatcgg gccggtatcc agccccagcg cacggcaggc
    65521 gacgatcaga taggccgcct gcatggaact gttgcgaaac gctgtttctt cggcaagttg
    65581 tgggctggag gtaaaccaac tgcgggcatc gccgtgagga aacagctgtg gtaaccgttc
    65641 ataaaattca ctgtcccagg cgacgatagc ggtgacgggc gcggtcaggg ttttttgcag
    65701 attgccgctg gaaagcgccg ggcgcagacg ttcttttcct tctgcagtgc ggataaacac
    65761 gatccgtgcc ggagaacagt tagcagatgt cggcccccat ttcatcaggg cataaatctc
    65821 ccgtaacgtc tcatcgctga cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt
    65881 gaacagggtg ctaagcgcac ctgggctaac ggcttcgttc atagcaattc cttacagagc
    65941 ggcttcacgg tgatgtaaca ggctggcaag cccgttgagt aacagagcat taaacgtttc
    66001 gggatcggtc acgttgcagg cgtgtccgcc atagcgcatc accattttct ggctatcggg
    66061 cagggcggcg tgaagttcac tggaacatgc tgttggcacc agcagatcat cactggcgca
    66121 gatgatttgc accgggcagc ggatacgatc cgcatggtga ctaaagtcag cgcgtttgag
    66181 ggcgttaagt cgacgcagta aattattttt gccttgaaaa tgcgccagtg ccagcgcatc
    66241 ttctgcctca aggcgaggtg cgcgggccgc catccagtcg gcgggataga ggaacaacgg
    66301 ctgcgcttcc acccatgcct gcgcgccgcc gctatgtagt aactgttcgc gaacctgaaa
    66361 acagcggcgc gtatgggcgt ttattcgtag ccagccgtta acgctgacca gcacagttac
    66421 cgacgcggga taatccagcg ccagctgcat tcccaccagc gcaccgagcg catggccgac
    66481 caccgcgtaa cgctcaatcc ctgcggctac cagcgcctga tgcagttccg ctgccatctg
    66541 ggcgatactg taatcttctg ccagcgtgtc gggattattg ccggtgccgc gctggtcgta
    66601 acagactacc tgatactcct gaaccagcac cgccagttgc ggtaaccagt aactgccgct
    66661 accgccaaga cccgaaatca acaccactac gggcgcatca gcataagggg gaggtgagag
    66721 tgaaagtttc atcgcggcct cacttggcga tatgcgcaat tgtggcgatt tccactagcg
    66781 cgtcaggttt taccagtccg cactgaatgc agaatcgcgc cggtttgtca cccgggaaaa
    66841 attcggcgta gatttcgtta atcgcggcgt aatttttcca gtcggtaata aagatgctgt
    66901 tgaaagtcac atccgccatt gtgccacccg ccgtctcgat gaccttgcgg atggtttcca
    66961 gaacgtggcg ggtttgcgcc tttgggtcat cggcaaacag cacgttgtta tgttgatcaa
    67021 aagccagcgt accggagaca tacaccacgc catcagccag cgtgccggga acgaaggggg
    67081 ccagcggtgc gctgctgcca gcaggaataa ttacggattt tggcatcgtt aaactcctta
    67141 agcgatacga gcaaaggacg tggaagaaag cgcgtcgcag aacgtttcga cgtcgctgac
    67201 ccagccaaaa aaggtttcga tattgaacaa cgcggctttc tgtgcaaatt ccggccccgc
    67261 ctggtgtgtt gcgtcttcca gcaccacgcc gaaatactcc agaaaaaagc cgtcgcgtag
    67321 cgtcgattct acgcagacgt tggtagcgat gccggtgaaa accagatggc gtattccgcg
    67381 gctgcgcaaa atgctgtcca gcggcgtatt gaagaaaccg ctgtagcgcg gcttcggcag
    67441 cactatatcg ccaggctgcg gcaccagctc atccaccagt tgataatccc aggagccttt
    67501 cgccagcaat ttcccctgca gcagcggctg attacgcatg gttttcaggg cgttcgattt
    67561 atgataattc ggtgagccgg ggccgccagc ctcgacatac tgttcatccc agccattttg
    67621 aaaccagatg atcagcatcc ctgccgttcg cgctgcggtc acggcggttt gaatgttggc
    67681 aatgaccggg cgagtggttg agacatcaaa cccggcgaga tctaagtagc cgcctggcgt
    67741 ggcataggca ttttgcatat ccaccacgat cagcgcagtt tgctgcggat cgaaggtaat
    67801 ggcttccggt cgtgcggtta aggtcgtcat catgccacct cctgagtcag cgcagggaga
    67861 tgggcgcggc actgcatcag tggttgaatg cgctcgccga agttttcgat tcccgacaga
    67921 aaatcatcga aggtcaacag cacgccttcg gcaccaggca cgcttgcgac ttcatctaac
    67981 atgcgcgcga cactggcgta agaaccgact aacgtcccca tattgatgtt taccgccgaa
    68041 gtgggatcgg ccatctgacg gacgttggtg tcagtacctg agcgggtatc tttctgactt
    68101 tgttcagtta gccagcttaa cgcctcttca tccgcgcccg ctttgtagtg ttcccatttt
    68161 gcacgagcgg catcgtcggt ttcgtcggca atcaccataa acaacacata agagccaacg
    68221 tcgcgtccgg tttgctctgc ggcctgtttc atccgcgcag cggtcggggc gaaagccgtg
    68281 ggtgtattta cgcctttgcc gaaacagaag ttaaaatcgg cataccgggc ggagaacgcc
    68341 atgccagcgt cgctttgccc ggcgcagatc actttcatgg ggacactcgg ttgcggactg
    68401 acgcgacaat catccatagt gaaaaaatcg cctttaaaat cgcttttgcc cgagcctcac
    68461 aggtcgcgca gcacctgaac atattcggtg agatagtcgt aacgacggga gaaatagtca
    68521 tcgccaggcc agatccccat ctgctcatac tcgggcttct gccagccagt cacgaggttg
    68581 acgccaaaac gcccgccaga gatggagtcg atggttgcgg ccatacgggc gacgattgcc
    68641 ggaggtaacg ttaaggtggc agctgtcgcg tatatctgaa tgcgtgaggt gacggctgcc
    68701 agccccgcca tcaaggtgaa cgactcaagg ttgtgatccc agaactcagt tttgccgcca
    68761 aagccacgca gtttgatcat cgacagggcg aaatcgaaat ggtagtgctc cgctttttgc
    68821 acaatggctt tattcagttc aaaggtcggc atgtactgcg gcgcgtgggt cgaaatgagc
    68881 cagccgttgt tgccaatagg tacgaatacg ccaattttca tcatcaacct ctcttcgtct
    68941 cgtaaagtga aagtcagacg gggcgctgca tcctgcatat ccttttcagc cgcgtaatgg
    69001 cttgtttgca aagcggatgc cagttttaaa aaagtgaatg ttattaatgt gttaatattg
    69061 cgttatctaa gatgtctggt aaaaagtgga ctaaacggtc aaaacagttg cacataaaac
    69121 atgcattcgt gcgcgatgag agtgcagaag gtcgaggccg ggcgggggtt ttgctatcct
    69181 gttgccaatc aacaagaggg gagagcgcat gacgcaaggc gcagtgaaaa caacgggtaa
    69241 gcgttcgcgc gcagtaagcg cgaagaaaaa agcgattctt agcgcagcac tggacacttt
    69301 ttcacaattc ggttttcacg gcacaaggct ggagcagatc gcggagttgg cgggtgtttc
    69361 aaaaaccaat ctgctgtatt actttccgtc aaaagaggcg ctgtatatag ccgtactgcg
    69421 gcagattctc gatatctggc tggcaccgtt aaaagcgttt cgagaagatt tcgccccgct
    69481 ggcggcgatc aaagagtaca tccgtctgaa gctggaagtt tcccgcgatt atccgcaggc
    69541 ttcgcgcctg ttctgtatgg agatgctggc aggtgcaccg ctgttaatgg atgaactgac
    69601 gggcgatttg aaggcattaa ttgatgagaa atcggcgctg attgccggtt gggttaaaag
    69661 cggcaaactc gcgccgattg acccgcagca tttgattttt atgatttggg cttccactca
    69721 acattacgcc gattttgccc ctcaggtgga ggcggtgaca ggcgcgacgt tgcgcgatga
    69781 ggtgtttttc aatcaaacgg ttgaaaacgt gcagcggatt attattgagg ggattcgacc
    69841 acgttaaaga tgccggagga ggttgtaaca tactccggct acctgtttaa ccgatcgtca
    69901 ttaaactggc gttaccgcca gcagcagcgg tattcacact cagcgaacgc tcgatataca
    69961 gccgttccag aaggatattg ctttcgccac gggcaaaacc ctgcaccgaa acaattgcac
    70021 catcccgtgc ggcaacggct tcacacaatg cgcgaagctg atccgaatca ccgtggaaga
    70081 tcaccgcatc aaagggttga gcggttatat tttccgcttt cgccagttga atacgttcgc
    70141 tgactgccga tggcaatgcc ttcactaact gacgatgcag cgcatcatcc ggccacagca
    70201 cctggctacc caccgccagc acggcggcga gctgagtcag cgcatcctgt tcatcatccg
    70261 caatacacaa cacgcgctca cgcggcagca gcgtccaggt gttgcgttca cccgtcggcc
    70321 ctggcaacaa tcgttgtgtt cctgcctgcg ccaactcgcc atattgcgta cataacgcct
    70381 gcaattctgg acggtttgcc gcccattccc gcagtgcatt tagcggctga gtcaatgcgg
    70441 ctttcaactg cgcatcgacc ggatactccg catcctgacg cgcgagcgtc accgccagcg
    70501 cactttccgg gcgattcgcc agcagacggt agagatagag cggaccgcct gctttcggcc
    70561 cggtaccgga caacccttcg ccgccgaacg gttgcacccc aaccaccgcg cccaccatat
    70621 tacggttaac gtacagatta ccgacctggg ccgagccagt gacctgggca atggtttcat
    70681 caatacgcgt atgaacgcca agcgtcagac catagccgga agcgttaatc tgctcgatca
    70741 gctctgggag ctggttacgg ttgtaacgca ccacatgcag caccggacca aagacctctt
    70801 tttgtaattc ggcaaagtca tccagttcga tcagcgtcgg agcgacaaag gtgccgcttt
    70861 gccattcacg ggcatcttcg ctgttttccc gcaccgcctg gaacacctga cggcctttac
    70921 tacgcatggt ctgaatatga cgctcgatat tggctttcgc ttcgctatca atcaccggac
    70981 cgatatcggt ggtcaggcga cccggattac ccatccggca ttcggccatt gcgccgcgca
    71041 gcattttcag cgtgtggtcg gcaatctcat cttgcaggca cagcacgcgc agtgccgaac
    71101 aacgctgacc tgcactgtcg aacgccgagg ccagcacatc tattacgact tgttcggtca
    71161 gtgctgaaga atcgacaatc atcgcgttca tgccgccggt ttcagcgatg agcggaatag
    71221 ggcgaccctg agcgtccagg cggctggcga tattgcgctg cagtaacgta gcgacttcgg
    71281 ttgaaccggt aaacatcacc ccgcgcacgc gatcatcacc cgtcagttgc gcgcccacgg
    71341 tttcaccccg acctggcagc aattgcacca cgcctggcgg tacacccgct tccagcaaaa
    71401 tggcgatccc ttgcgcggca atcagcggcg tttgttctgc cggttttgcc agcacgctgt
    71461 tacctgccgc cagtgcggca gcgatctgtc cggtgaaaat agccagcggg aagttccacg
    71521 gactgataca taccacaggc cctaatggac ggtgggtttc gttagcgaaa tcatcccgca
    71581 cctgtccggc atagtagtgg agaaaatcga ccgcttcgcg cacttcggca atggcgttac
    71641 tgaaggtttt tccggcttca cgcaccagaa taccaatcag ttgctgcatc tggctttcca
    71701 ttagcacggc agcgcgatgc aaaatcgccg cgcgttcagc cggaggagtg gcaaaccaga
    71761 ttggcgcgtt attaaccgca ctttccagcg cctgttctac ttcacgcggc gtggcttcac
    71821 gcacatagcc cacaatatct ttcggttccg cagggttaat aacgggcgac atctcaccag
    71881 ccgcgaccga ttgttccagc attggcaagg actgccattt ttgcagtgca ctattgagca
    71941 gggctgaaga gagcgaggcc agacggtgtt cgttagcgag atccagccct gccgagttgt
    72001 cgcgcccgtg accgtaaaga tcgcgcggca ggggaatttt cggatgcggt aatccagttt
    72061 gcccttcctg ttgcgccagt ttttctacag cagtgactgg atcggcgacc agttcatcca
    72121 gtggcaaaga ggtgtcggca atacggttaa caaacgaggt gttagcaccg ttttccagca
    72181 ggcggcgcac cagatatgcc agcagcgttt catgtgtacc gaccggagca taaatacgac
    72241 acggacggtt aagtttgccg tcggcaactt tcccggtgac ctgctcatac agtggctcgc
    72301 ccataccatg caggcactgg aactcgtact gacccgggta gtagttttga cccgccagtt
    72361 gataaattgc cgccagcgta tgggcgttgt gcgtcgcgaa ctgcgggtag attagatttg
    72421 gcaccgccag cagttttttc gcacaggcaa gataagaaac gtcggtatac accttgcggg
    72481 tataaaccgg ataaccttca aggccgtcca tctgtgcacg cttgatttca ctatcccagt
    72541 acgcgccttt caccaggcga atcatcagac ggcgacggct gcgggtggcg agatcaatca
    72601 ggtaatcgat caccaacggg cagcgttttt gataagcctg aataacaaaa ccgatgccgt
    72661 tccagcctgc cagttcaggc tcgaaacaga gtttttccag cagatcgagg gagatctcca
    72721 ggcgatcggc ctcttcggca tcgatgttaa taccaatatc gtactgacgc gccagcaggg
    72781 tgagtgattt cagacgcggg taaagctctt ccattacccg gtcatactgg gcgcggctat
    72841 aacgcggatg tagcgccgac agcttgattg aaatgcccgg accttcatag atgccacgac
    72901 cgttagacgc tttaccaatg gcgtgaatcg cctgctgata ggaaaccata tacgcttgtg
    72961 catctgcggc agtcagtgcg gcttcgccca gcatatcgta agagtaacgg aaacctttct
    73021 cttccagttt gcgggcattg gctaatgctt ccgcgatggt ttcgcccgtg acgaactgct
    73081 cgcccatcag gcgcatcgcc atatccacac ctttgcggat cagcggttca ccgctcttac
    73141 cgataatgcg gttcagcgag cgggagaggc tggcttcgtt atgggtggaa accagtttgc
    73201 cggtaaacag cagcccccag gtagcggcat taacaaacag ggacgggcta cgaccaatgt
    73261 gtgactgcca gttaccgtta ctgattttgt cgcgaattaa cgcgtcacgg gtggctttgt
    73321 cgggaatacg caacaacgct tccgccagac acatcagcgc cacaccttcc tgcgatgaca
    73381 acgaaaactc ctgcaataac ccctgaacca tacctgcgcg accactggcg tttttttgat
    73441 tacgcagttt atcggccagt tgatacgcca gtttgtgcgc ctgttcagca actggctgcg
    73501 gcaggcgagc ttgttccagc agcatagaaa ccgcttcggt ttccgggcgg cgataggccg
    73561 cggtgatcgc ggcgcgggaa actgactggg gcaatatttg ctcggcaaag tcgaggaaag
    73621 gctggtgtgg ttcctctgcc ggagtcggtg cttcatcgct ctcattggcc gcgccagaaa
    73681 gcagcgcagg tagctccggg agagtatcgc tgttttccag ttgttcaaga taagaaaaaa
    73741 tcgcctgttt aattaaccag tgtggtgtgc gatcgatacg tgtcgcggca gacttaatac
    73801 gctcacgcgt cgcgtcgtcc agcttaaccc ccatggtggt ggttcccatg ccattactcc
    73861 tgttgttctg aaaggtgcaa cttaacgtta tcgtgaaata tccatgatgt tgcaactttg
    73921 tgcaaccgtg ttaaatgtga cctgcgtagc aagcttaaaa atgaatgaaa tgttaataaa
    73981 cgaaatcgat atgacaggga ttaaaaaaat aactcagact ttttctctgc ggcagttaac
    74041 atttttgaaa ggtgcaaccg caaaaaatgt gagagagtgc aacctgatga aaaatagtgt
    74101 cgctgagcac taaaatttaa tgtaaatggt gtgttaaatc gattgtgaat aaccagcgct
    74161 tccggcagga tacggtcgcc ctggtaaaac ataaactctg ttaccccgtt ccggtggcag
    74221 atataacggc aagtttcgac attgccgata ataatttttt ggagacttta gatggctatt
    74281 agcacaccga tgttggtgac attttgtgtc tatatctttg gcatgatatt gattgggttt
    74341 atcgcctggc gatcaacgaa aaacttcgac gactatattc tgggcggtcg tagtcttggg
    74401 ccattcgtga cggcattatc ggcgggtgcg tcggatatga gcggctggct gttaatgggg
    74461 ttgccaggcg ctgtttttct ttccgggatt tccgaaagct ggatcgccat tggtctgaca
    74521 ttaggcgcgt ggatcaactg gaagctggtg gccgggcggt tgcgtgtgca taccgaatac
    74581 aacaataacg ccttaacatt gccggattat ttcaccgggc gctttgaaga taaaagccgc
    74641 attttgcgca ttatctccgc gctggttatt ttgctgttct tcaccattta ttgcgcttcg
    74701 ggcattgtgg caggtgcgcg tctgtttgaa agtacctttg gcatgagcta cgaaacggct
    74761 ctgtgggcgg gggctgcggc gacgatcctt tacaccttta ttggcggttt cctcgcggtg
    74821 agctggactg acactgtaca ggccagtctg atgatttttg ccctgatcct gacgccggtt
    74881 atcgtgatca ttagcgtcgg tgggtttggc gactcgctgg aagtaatcaa acaaaagagc
    74941 atcgaaaacg tggatatgct caaagggttg aattttgtcg ccattatctc gctgatgggg
    75001 tgggggctgg gttacttcgg gcagccgcac atcctggcgc gttttatggc ggcagattct
    75061 caccacagca ttgtccatgc gcgtcgtatc agtatgacct ggatgatcct ctgcctggca
    75121 ggggcggtgg ctgtcggctt ctttggtatc gcttacttta acgagcaccc ggcggtagca
    75181 ggtgcggtaa accagaacgc tgaacgcgtg tttattgaac tggcgcaaat tctgtttaac
    75241 ccgtggattg ccgggatttt gctgtcggcg attctggcgg cggtaatgtc aacgttaagt
    75301 tgccagctgc tggtatgttc cagtgcgatt accgaagatt tatacaaagc gtttctgcgt
    75361 aaacaggcca gccagaaaga gctggtgtgg gtagggcgag tgatggtgct ggtggtggcg
    75421 ctggtggcga ttgcgctggc ggcaaacccg gaaaaccgcg tgctgggctt agtgagctac
    75481 gcgtgggcag gctttggcgc agcgtttggt ccggtggtgc tgttctcggt gatgtggtca
    75541 cgcatgacgc gcaacggtgc gctggcgggg atgatcatcg gtgcgctgac ggttatcgtc
    75601 tggaaacagt tcggctggct gggtctgtac gaaattattc cgggctttat cttcggcagt
    75661 atcgggattg tagtgtttag tttgctgggt aaagcgccgt cagcggcgat gcaaaaacgc
    75721 tttgccgagg ccgatgcgca ctatcattcg gctccgccgt cacggttgca ggaagggtaa
    75781 gggaaggcgt tttaccggaa atttcacgtc ataacaggaa gcaggcagag ggcaatcagt
    75841 gattgccctc tgcgtttttt ctcaaaatat ttttacatct tctcatcatt atcactacat
    75901 tccacttgta tttttcttca tcgtaatgat aatcgctatc actgcgattt acttttcttt
    75961 gcatagattg actcagaaaa acgtttaagg gtgggtggca tgtttgttcc gtttctcatt
    76021 atgttgcgcg aaggacttga agccgcgctg attgtcagtt tgattgccag ctatcttaag
    76081 cgtacccagc gaggccgatg gattggtgta atgtggattg gcgtgttgct tgccgctgcg
    76141 ttgtgcctgg gcttgggtat cttcattaac gaaaccaccg gcgaatttcc gcaaaaagaa
    76201 caggaactgt ttgaaggcat tgtggcggtg atcgccgtgg tgatccttac ctggatggtt
    76261 ttctggatgc gcaaagtgtc gcgcaacgtc aaagtacaac tggagcaggc ggtcgatagc
    76321 gcattgcagc gtggaaatca tcatggctgg gcgctggtga tgatggtctt ttttgccgtt
    76381 gcaagggaag ggctggagtc ggtctttttc ctgctggcgg catttcaaca agatgtcggg
    76441 atctggccgc cgctgggtgc aatgcttggt cttgctacag ccgtggtgct tggcttcctg
    76501 ctctactggg gcggtattcg cctcaatctt ggtgcatttt ttaaatggac cagtctgttt
    76561 attctcttcg tcgccgcagg gctggcagct ggtgccattc gcgcatttca tgaagccgga
    76621 ttgtggaacc actttcagga aatcgccttc gatatgagcg cggtgctctc aactcactcg
    76681 ctgtttggca cactgatgga agggattttt ggctatcagg aagcaccgag cgtcagcgaa
    76741 gtcgccgtct ggtttattta tctcatcccg gcgctggtgg catttgttct gccaccacgc
    76801 gcaggggcga ctgcgtcccg ctccgtgtag caaatacgac gcaaactctt gcttagttac
    76861 aacatacttt aaagggatag tctcgtcatg accattaact tccgccgtaa cgcattgcag
    76921 ttgagcgtgg ctgcgctgtt ctcttctgct tttatggcta acgccgctga tattccgcaa
    76981 gtcaaagtga ccgtgacgga taagcagtgc gagccgatga ccattacggt taacgccggg
    77041 aaaacacagt tcattattca gaaccacagc cagaaggcgc tggagtggga gatcctcaaa
    77101 ggcgtgatgg tggtggaaga gcgggaaaat atcgcccctg gttttagcca gaaaatgacg
    77161 gcgaatttac agcctggcga atacgatatg acctgcggtc tgctgactaa cccgaaaggg
    77221 aagttgatcg tcaaaggtga agcaacggcg gatgcggcgc aaagtgatgc gctgttaagt
    77281 cttggtggtg caattactgc atataaagcg tatgtcatgg cggaaaccac gcaactggtg
    77341 accgacacca aagcctttac tgacgctatt aaagcaggcg atatcgaaaa agcgaaagca
    77401 ctgtatgcgc cgacgcgcca gcactatgag cgcatcgaac cgattgctga actgttctcc
    77461 gatctggatg gcagcattga cgcccgtgaa gatgattacg agcaaaaagc cgccgatccg
    77521 aaattcaccg gtttccaccg tctggaaaaa gaattgtttg gcgataacac caccaaaggg
    77581 atggataagt atgctgacca gctttatacc gatgtggtcg atttgcaaaa acgcatcagt
    77641 gaactggctt tccctccttc aaaagtggtc ggcggtgcag ccggactgat tgaggaagtg
    77701 gcagccagca aaatcagcgg tgaagaagat cgctacagcc acaccgatct gtgggatttc
    77761 caggctaacg ttgaaggctc gcagaaaatt gtcgatctgc tgcgtccaca actacaaaaa
    77821 gcgaatccgg aactgttggc aaaagtcgat gccaacttca aaaaggtcga taccattctg
    77881 gcgaaatacc gtactaaaga cggttttgaa aattacgaca aactgaccga tgctgaccgg
    77941 aatgcactga aaggaccgat tactgcgctg gcggaagatc tggcgcaact tcgcggtgtg
    78001 ctggggctgg actaagcgtt atgcagtatg aagatgaaaa cggcgtgaat gaaccgtcac
    78061 gccgacgttt actgaaaggg attggtgcgc tggcgctggc gggaagttgt ccggtcgctc
    78121 atgcacaaaa aacgcaaagt gcgccgggta cgctttcacc ggatgcacgc aatgagaaac
    78181 agccgtttta tggtgagcat caggcaggga tcctgacacc acaacaggct gcaatgatgc
    78241 tggtggcgtt tgatgtgctt gccagcgata aagccgatct tgaacggttg tttcgcttgt
    78301 tgactcagcg ttttgctttt ctgactcagg gcggagcggc accagaaacg ccaaatccgc
    78361 gcctgccacc actcgattcc ggcattcttg gcggctacat tgcgcccgat aatctcacca
    78421 tcacgttatc ggtgggtcac tcattgtttg atgagcgctt tggccttgcg ccacagatgc
    78481 cgaaaaagtt gcagaagatg acgcgtttcc ccaacgactc gctggatgcg gcgttatgtc
    78541 atggtgatgt gttgctacaa atttgcgcca acacccagga cacggtgatc catgcgctgc
    78601 gcgatatcat caaacacacg ccggatttgc tcagcgtgcg ctggaagcgg gaagggttta
    78661 tttccgacca cgcagcgcgt agtaaaggca aagagacgcc gataaatttg ctgggcttta
    78721 aagacggcac cgccaatccc gatagccaga atgcgaagtt gatgcaaaaa gtggtgtggg
    78781 tgacggcaga tcagcaggag cctgcgtgga caatcggtgg cagctatcag gcagtgcgct
    78841 tgattcagtt tcgagtggaa ttttgggaca gaacgccgct gaaagagcag cagacgatat
    78901 ttggtcgtga taagcaaacc ggtgcgccgc tgggcatgca gcacgagcat gatgttcccg
    78961 attacgctag cgatccggaa gggaaggtga ttgcgctgga cagccatatc cggctggcga
    79021 atccccgcac ggcggagagc gagtccagcc tgatgctgcg tcgtggctac agttattcgt
    79081 tgggcgtcac caattccggg caactcgata tggggctact gtttgtctgc taccaacacg
    79141 atctggaaaa gggcttcctg acagtacaaa aaaggttgaa tggcgaggcg ctggaggaat
    79201 atgtcaaacc tatcggtggc ggctatttct ttgcgctgcc gggtgtgaag gacgcgaacg
    79261 attatctcgg aagcgcatta ttgcgggttt aatgttttta ggcggataag gcgtttacgc
    79321 cgcaccaggc gttcgtacac tgatgcgacg cttgcgcgtc ttatcatgcc tacaatcggt
    79381 gcgggtcggt aggccggata aggcgttcac gccgcatccg gcaagctttt accagtactg
    79441 cgaatcctgc tgaaagcaca cagctttttt catcactgtc atcactctgt catctttcca
    79501 gtagaaacta atg
//
LOCUS       DADTFG010000003        77307 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.003, whole genome shotgun sequence.
ACCESSION   DADTFG010000003 DADTFG010000000
VERSION     DADTFG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.003"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            38..481
                     /gene="cmtB"
                     /locus_tag="J1138_000212"
     CDS             38..481
                     /gene="cmtB"
                     /locus_tag="J1138_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBA8452063.1"
                     /translation="MRLIDYFPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQ
                     AIKDSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            509..1897
                     /locus_tag="J1138_000213"
     CDS             509..1897
                     /locus_tag="J1138_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBA8452064.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEVPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESDDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1912..3195
                     /locus_tag="J1138_000214"
     CDS             1912..3195
                     /locus_tag="J1138_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBA8452065.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLTQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIAIALSATG
                     QLQPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLF
                     KELARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3192..3507
                     /locus_tag="J1138_000215"
                     /pseudo
     CDS             3192..3507
                     /locus_tag="J1138_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3529..4038
                     /gene="fumE"
                     /locus_tag="J1138_000216"
     CDS             3529..4038
                     /gene="fumE"
                     /locus_tag="J1138_000216"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBA8452066.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMAWDFISNVNSIIRNASLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4035..4748
                     /locus_tag="J1138_000217"
     CDS             4035..4748
                     /locus_tag="J1138_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBA8452067.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLSPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(4720..5397)
                     /locus_tag="J1138_000218"
     CDS             complement(4720..5397)
                     /locus_tag="J1138_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA8452068.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(5391..6068)
                     /locus_tag="J1138_000219"
     CDS             complement(5391..6068)
                     /locus_tag="J1138_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBA8452069.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(6056..6763)
                     /locus_tag="J1138_000220"
     CDS             complement(6056..6763)
                     /locus_tag="J1138_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBA8452070.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(6764..7342)
                     /locus_tag="J1138_000221"
     CDS             complement(6764..7342)
                     /locus_tag="J1138_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBA8452071.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7369..7797)
                     /locus_tag="J1138_000222"
     CDS             complement(7369..7797)
                     /locus_tag="J1138_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA8452072.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQSCALSGYDELHISPR"
     gene            8169..9188
                     /gene="epd"
                     /locus_tag="J1138_000223"
     CDS             8169..9188
                     /gene="epd"
                     /locus_tag="J1138_000223"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBA8452073.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDTTVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9238..10401
                     /gene="pgk"
                     /locus_tag="J1138_000224"
     CDS             9238..10401
                     /gene="pgk"
                     /locus_tag="J1138_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBA8452074.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            10616..11695
                     /gene="fbaA"
                     /locus_tag="J1138_000225"
     CDS             10616..11695
                     /gene="fbaA"
                     /locus_tag="J1138_000225"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBA8452075.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            11886..12746
                     /locus_tag="J1138_000226"
     CDS             11886..12746
                     /locus_tag="J1138_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBA8452076.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAV"
     gene            12885..13520
                     /gene="argO"
                     /locus_tag="J1138_000227"
     CDS             12885..13520
                     /gene="argO"
                     /locus_tag="J1138_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBA8452077.1"
                     /translation="MFSYYFQGLVLGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            13613..14353
                     /locus_tag="J1138_000228"
     CDS             13613..14353
                     /locus_tag="J1138_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBA8452078.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDVYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            14521..15417
                     /locus_tag="J1138_000229"
     CDS             14521..15417
                     /locus_tag="J1138_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8452079.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASRFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSRDIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTIQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEK
                     LTISELPPLRLGFHVSRRYEGRQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(15414..16508)
                     /locus_tag="J1138_000230"
     CDS             complement(15414..16508)
                     /locus_tag="J1138_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBA8452080.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTTQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLAAHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISSYHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDGFVPRLSFF
                     FGIGMDLFMNAAMLRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMCRDYLHRYL
                     ENASGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(16711..17604)
                     /locus_tag="J1138_000231"
     CDS             complement(16711..17604)
                     /locus_tag="J1138_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HBA8452081.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            17745..17975
                     /locus_tag="J1138_000232"
     CDS             17745..17975
                     /locus_tag="J1138_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452082.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            18031..18690
                     /gene="rpiA"
                     /locus_tag="J1138_000233"
     CDS             18031..18690
                     /gene="rpiA"
                     /locus_tag="J1138_000233"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBA8452083.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            18946..20178
                     /locus_tag="J1138_000234"
     CDS             18946..20178
                     /locus_tag="J1138_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBA8452084.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            20407..20466
                     /gene="ibsC"
                     /locus_tag="J1138_000235"
     CDS             20407..20466
                     /gene="ibsC"
                     /locus_tag="J1138_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBA8452085.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(20568..21170)
                     /locus_tag="J1138_000236"
     CDS             complement(20568..21170)
                     /locus_tag="J1138_000236"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBA8452086.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHMVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVVTPSKVWEW"
     gene            complement(21191..21374)
                     /gene="ssrS"
                     /locus_tag="J1138_000237"
     ncRNA           complement(21191..21374)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="J1138_000237"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(21416..21745)
                     /gene="zapA"
                     /locus_tag="J1138_000238"
     CDS             complement(21416..21745)
                     /gene="zapA"
                     /locus_tag="J1138_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBA8452087.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            21913..22491
                     /gene="ygfB"
                     /locus_tag="J1138_000239"
     CDS             21913..22491
                     /gene="ygfB"
                     /locus_tag="J1138_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBA8452088.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            22517..23842
                     /gene="pepP"
                     /locus_tag="J1138_000240"
     CDS             22517..23842
                     /gene="pepP"
                     /locus_tag="J1138_000240"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBA8452089.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            23839..25017
                     /gene="ubiH"
                     /locus_tag="J1138_000241"
                     /gene_synonym="visB"
     CDS             23839..25017
                     /gene="ubiH"
                     /locus_tag="J1138_000241"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBA8452090.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVSRTQSHVEVTLESGGTLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLTLTRTARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25040..26242
                     /gene="ubiI"
                     /locus_tag="J1138_000242"
                     /gene_synonym="visC"
     CDS             25040..26242
                     /gene="ubiI"
                     /locus_tag="J1138_000242"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBA8452091.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKARQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(26316..26399)
                     /locus_tag="J1138_000243"
     CDS             complement(26316..26399)
                     /locus_tag="J1138_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452092.1"
                     /translation="MSKNTKSKIRGIRKYNAKSQVKLVYFK"
     gene            26690..27784
                     /gene="gcvT"
                     /locus_tag="J1138_000244"
     CDS             26690..27784
                     /gene="gcvT"
                     /locus_tag="J1138_000244"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBA8452093.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            27808..28197
                     /gene="gcvH"
                     /locus_tag="J1138_000245"
     CDS             27808..28197
                     /gene="gcvH"
                     /locus_tag="J1138_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBA8452094.1"
                     /translation="MSNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            28315..31188
                     /gene="gcvP"
                     /locus_tag="J1138_000246"
     CDS             28315..31188
                     /gene="gcvP"
                     /locus_tag="J1138_000246"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBA8452095.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(31354..32787)
                     /locus_tag="J1138_000247"
     CDS             complement(31354..32787)
                     /locus_tag="J1138_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA8452096.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            32832..33143
                     /locus_tag="J1138_000248"
     CDS             32832..33143
                     /locus_tag="J1138_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBA8452097.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            33307..33966
                     /locus_tag="J1138_000249"
     CDS             33307..33966
                     /locus_tag="J1138_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBA8452098.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(34043..35023)
                     /gene="ygfZ"
                     /locus_tag="J1138_000250"
     CDS             complement(34043..35023)
                     /gene="ygfZ"
                     /locus_tag="J1138_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBA8452099.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            35276..35542
                     /gene="sdhE"
                     /locus_tag="J1138_000251"
     CDS             35276..35542
                     /gene="sdhE"
                     /locus_tag="J1138_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBA8452100.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            35523..35930
                     /locus_tag="J1138_000252"
     CDS             35523..35930
                     /locus_tag="J1138_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452101.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(35970..36491)
                     /gene="fldB"
                     /locus_tag="J1138_000253"
     CDS             complement(35970..36491)
                     /gene="fldB"
                     /locus_tag="J1138_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBA8452102.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            36603..37499
                     /gene="xerD"
                     /locus_tag="J1138_000254"
     CDS             36603..37499
                     /gene="xerD"
                     /locus_tag="J1138_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBA8452103.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            37524..38234
                     /gene="dsbC"
                     /locus_tag="J1138_000255"
     CDS             37524..38234
                     /gene="dsbC"
                     /locus_tag="J1138_000255"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBA8452104.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            38240..39973
                     /gene="recJ"
                     /locus_tag="J1138_000256"
     CDS             38240..39973
                     /gene="recJ"
                     /locus_tag="J1138_000256"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBA8452105.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNCS
                     LQIIIDNIWPI"
     gene            40064..41162
                     /gene="prfB"
                     /locus_tag="J1138_000257"
     CDS             join(40064..40138,40140..41162)
                     /gene="prfB"
                     /locus_tag="J1138_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBA8452106.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            41172..42689
                     /gene="lysS"
                     /locus_tag="J1138_000258"
     CDS             41172..42689
                     /gene="lysS"
                     /locus_tag="J1138_000258"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBA8452107.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(42732..43280)
                     /gene="idi"
                     /locus_tag="J1138_000259"
     CDS             complement(42732..43280)
                     /gene="idi"
                     /locus_tag="J1138_000259"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBA8452108.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            43335..43406
                     /locus_tag="J1138_000260"
     CDS             43335..43406
                     /locus_tag="J1138_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452109.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(43403..43528)
                     /locus_tag="J1138_000261"
     CDS             complement(43403..43528)
                     /locus_tag="J1138_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452110.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(43530..44978)
                     /locus_tag="J1138_000262"
     CDS             complement(43530..44978)
                     /locus_tag="J1138_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBA8452111.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            45414..47333
                     /gene="ygfT"
                     /locus_tag="J1138_000263"
     CDS             45414..47333
                     /gene="ygfT"
                     /locus_tag="J1138_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBA8452112.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            47333..47821
                     /locus_tag="J1138_000264"
     CDS             47333..47821
                     /locus_tag="J1138_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA8452113.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(47857..49224)
                     /locus_tag="J1138_000265"
     CDS             complement(47857..49224)
                     /locus_tag="J1138_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA8452114.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(49260..50576)
                     /gene="guaD"
                     /locus_tag="J1138_000266"
     CDS             complement(49260..50576)
                     /gene="guaD"
                     /locus_tag="J1138_000266"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HBA8452115.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(50594..51994)
                     /gene="xanQ"
                     /locus_tag="J1138_000267"
     CDS             complement(50594..51994)
                     /gene="xanQ"
                     /locus_tag="J1138_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HBA8452116.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(52159..55029)
                     /locus_tag="J1138_000268"
     CDS             complement(52159..55029)
                     /locus_tag="J1138_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBA8452117.1"
                     /translation="MIIHFTLNGAPQELTVNPGKNVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(55026..55805)
                     /locus_tag="J1138_000269"
     CDS             complement(55026..55805)
                     /locus_tag="J1138_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBA8452118.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIATRQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(55856..57184)
                     /gene="ssnA"
                     /locus_tag="J1138_000270"
     CDS             complement(55856..57184)
                     /gene="ssnA"
                     /locus_tag="J1138_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBA8452119.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     SGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHVAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(57187..60285)
                     /gene="ygfK"
                     /locus_tag="J1138_000271"
     CDS             complement(57187..60285)
                     /gene="ygfK"
                     /locus_tag="J1138_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBA8452120.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLSISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(60607..61185)
                     /gene="mocA"
                     /locus_tag="J1138_000272"
     CDS             complement(60607..61185)
                     /gene="mocA"
                     /locus_tag="J1138_000272"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBA8452121.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            61289..62059
                     /gene="yqeC"
                     /locus_tag="J1138_000273"
     CDS             61289..62059
                     /gene="yqeC"
                     /locus_tag="J1138_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBA8452122.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            62107..63732
                     /locus_tag="J1138_000274"
     CDS             62107..63732
                     /locus_tag="J1138_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBA8452123.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(63773..64705)
                     /gene="arcC"
                     /locus_tag="J1138_000275"
     CDS             complement(63773..64705)
                     /gene="arcC"
                     /locus_tag="J1138_000275"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA8452124.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(64753..66138)
                     /locus_tag="J1138_000276"
     CDS             complement(64753..66138)
                     /locus_tag="J1138_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HBA8452125.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRQQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGILAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(66191..67402)
                     /locus_tag="J1138_000277"
     CDS             complement(66191..67402)
                     /locus_tag="J1138_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBA8452126.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(67460..68656)
                     /gene="dpaL"
                     /locus_tag="J1138_000278"
     CDS             complement(67460..68656)
                     /gene="dpaL"
                     /locus_tag="J1138_000278"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBA8452127.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDII
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(68714..69901)
                     /gene="ygeW"
                     /locus_tag="J1138_000279"
     CDS             complement(68714..69901)
                     /gene="ygeW"
                     /locus_tag="J1138_000279"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBA8452128.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            70380..72158
                     /locus_tag="J1138_000280"
     CDS             70380..72158
                     /locus_tag="J1138_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBA8452129.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQERCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANASDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(72198..72677)
                     /gene="xdhC"
                     /locus_tag="J1138_000281"
     CDS             complement(72198..72677)
                     /gene="xdhC"
                     /locus_tag="J1138_000281"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBA8452130.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(72674..73552)
                     /gene="xdhB"
                     /locus_tag="J1138_000282"
     CDS             complement(72674..73552)
                     /gene="xdhB"
                     /locus_tag="J1138_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBA8452131.1"
                     /translation="MFDFASYHRAATLADAITLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELQGITQAEDGALRIGSATTFTQLIEDPVIQRNLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLELHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(73563..75860)
                     /gene="xdhA"
                     /locus_tag="J1138_000283"
     CDS             complement(73563..75860)
                     /gene="xdhA"
                     /locus_tag="J1138_000283"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBA8452132.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITSPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLIERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            76274..77029
                     /locus_tag="J1138_000284"
     CDS             76274..77029
                     /locus_tag="J1138_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBA8452133.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            77108..77181
                     /locus_tag="J1138_000285"
     tRNA            77108..77181
                     /locus_tag="J1138_000285"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77140..77142,aa:Gly,seq:ccc)
BASE COUNT        18787 a        19116 c        20388 g        19016 t
ORIGIN      
        1 cgccggacgg taccccaaaa atgaataagg ataatttatg cggcttattg attattttcc
       61 agaattatca atctcagtta tacattcaac aaaagactgg caggaagcta tcaatttctc
      121 gatggcatca ctgttagcaa aaaactatat cagcgagaat tacattcagg ctattaaaga
      181 ttccaccatc agcaatggtc cttattatat tctcgcacca ggcgtggcaa tgcctcatgc
      241 gcgaccggaa tgtggagcgc ttaaaaccgg gatgtctttg acgttactta aacaaggcgt
      301 ttattttccg gggaatgacg aaccgattaa attactcatc ggactttctg ctgccgatgc
      361 cgattcgcac attggtgcta ttcaggcgtt aagtgaatta ctgtgcgaag aagaaatact
      421 cgaacaactc ttaaccgcat catcagaaaa acaattagcg gacattatca gccgcggata
      481 atgactctct cctctacagg ataatattat ggaaaacaag tctgctcgtg caaaggtcca
      541 ggcttttggg ggatttttga ctgcaatggt catccccaat atcggtgctt ttattgcctg
      601 gggatttata acggcattat ttattcccac cggttggctg ccgaatgaac atttcgccaa
      661 aattgttggt ccgatgatta cctatttatt gcccgtgatg attggttcta ccggtggtca
      721 tctggtcggc ggtaaacgcg gcgcggtcat gggcggaatt ggtacgattg gtgtgatcgt
      781 cggcgctgaa gtcccgatgt tccttggctc gatgattatg gggccgctcg gtggtctggt
      841 gattaaatat atcgacaagt cgctggaaaa acgcatacct gctggttttg agatggttat
      901 caataacttc tcattaggta tcgcggggat gctcctttgt ctgctgggtt ttgaagttat
      961 cggcccggcg gtgttaattg ctaatacttt cgtcaaagag tgtattgagg cgctggtaca
     1021 tgcgggttat ctacctttgc tgtcagtcat caatgaaccg gcgaaagtgc ttttccttaa
     1081 taatgcgatc gatcagggcg tctattatcc gctgggaatg caacaggctt cggttaacgg
     1141 taaatccatc ttcttcatgg tggcctccaa cccaggtcct ggtctggggc tgctgctggc
     1201 gtttaccttg tttggtaaag ggatgagtaa acgttctgcg cccggggcga tgattattca
     1261 cttcctcggt gggatccacg aactgtattt cccgtatgtg ctgatgaagc cgctgaccat
     1321 tattgccatg attgcgggcg gtatgtctgg cacctggatg tttaacttac tggacggtgg
     1381 tctggtggct ggcccaagtc ctgggtctat ctttgcttac ctggcactga cgccgaaagg
     1441 ctcgttcctg gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct ttgctatcac
     1501 ttcgctgata ctgaagatgg aaaaaacggt ggaaacggag agcgacgatg agtttgctca
     1561 gtcagccaat gcggttaagg cgatgaaaca agaaggtgca ttctcgttaa gccgtgttaa
     1621 gcgtattgcc tttgtttgcg atgcagggat gggctccagt gcgatgggcg caaccacctt
     1681 ccgtaaacgc ctggaaaaag cggggctggc aatagaagta aaacattacg ccatagaaaa
     1741 cgtgcctgcg gatgcggata tcgtcgttac tcacgccagt ctggaagggc gcgtgaaacg
     1801 tgtgacggat aaaccactga tattgattaa taactatatt ggcgatccaa aactcgacac
     1861 tttatttaat caattaaccg ctgaacataa acactgatta cagaggtaaa aatgaaaacc
     1921 aaagttgctg ctatttatgg caagcgggat gtccgtctgc gcgaatttga gctgccagaa
     1981 attaccgata atgaactgtt agtgaatgta atttctgaca gcgtttgttt atccacctgg
     2041 aaagcggcgt tactcggtag tgaacataaa cgcgtccccg acgatttaga aaatcatccg
     2101 gtcattaccg ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa tctcactggc
     2161 aaatataaaa aaggccagcg ttttgtattg caaccggcga tggggttacc aggcgggtat
     2221 tcagcgggct acagctacga atattttggc ggtaacgcca cctatatgat tattcccgaa
     2281 atcgccatta acctgggctg cgtattaccg tatcacggct cttattttgc tgcggcctcg
     2341 ctggcggaac cgatgtgctg cattattggc gcttatcatg ccaattatca taccacgcaa
     2401 tatgtttatg agcatcgcat gggcgtcaaa cctggtggca atattgcgct actggcgtgt
     2461 gcaggcccga tgggaattgg cgctattgat tacgccatta acggcggcat acaaccggcg
     2521 cgggtggtgg tagtcgatat cgacgacaaa cgcctgacgc aggtacaaaa gctgttgccg
     2581 gtggaactgg cggccagtaa aggcattgag ctggtgtata tgaataccaa agggatgagc
     2641 gatccggtcc agacgctgcg ggcgctgacg gatgatgccg gatttgatga cgtatttgtc
     2701 tatgcagcgg tgccttcagt cgtcgagatg gcggatgaat tactggctga agatggctgc
     2761 ctgaacttct ttgccggtcc gacggataaa aacttcaaag tgccgtttaa tttctacaac
     2821 gtccattaca acagtacgca cattgtcggc acgtccggcg gttccacgga cgacatgaaa
     2881 gaggcgattg cgattgccct tagcgccacg gggcagttac agccgtcgtt tatggtgacc
     2941 catatcggtg gcctggatgc ggtgccagaa accgtgctca atctgccgga tatccctggc
     3001 ggtaaaaaac tcatttataa cggcgtgacc atgccgctga ccgccattgc cgattttgcc
     3061 gaaaaaggca aaaccgatcc gctgtttaaa gagctggcgc ggctggttga gaaaacgcac
     3121 ggtatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt tgatattggg
     3181 gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa caggcagcgt
     3241 tggctgcctg gccgcaaacc ggctgtggcg acaaaaataa aattgatggc cttgcggtca
     3301 ccgcgatgcg ccaggcatta aacgatgttg cttttcgtgg gcgagtggtt atcggcgagg
     3361 gggaacgata tcctctttag cgctacgggc gtgacgggcg gcgagttggt gaacggtatc
     3421 caacagacgg cgaatggggt gcggacgcag acattactga tcggcggcgc ggaccaaacg
     3481 tgtaatataa tagactccct gcattgatgg tgatctatcg gaagaccaat ggcgacgctg
     3541 acagaagatg atgtgcttga gcaactgaat gcacaggaca atttattttc atttatgaaa
     3601 actgcgcatt ctattttgct ccaggggata cgccagtttc tgccgtcgct gtttgtcgat
     3661 aacgatgaag agatcgtcga atatgcagtg aagccgttac tcgcccaaag cggcccgctt
     3721 gacgatattg atgttgcgct gcgtttgatt tatgcgctgg ggaaaatgga taaatggctg
     3781 tacgccgata tcacgcattt ttcccagttt tggcattacc tgaacgaaca ggatgaaacg
     3841 cccggatttg ccgatgacat ggcctgggat tttatcagca acgtcaatag cataatccgc
     3901 aatgcgagtc tctatgacgc gttgaaagcg atgaaattcg ccgatttcgc cgtctggtca
     3961 gaggcgcgtt ttagcgggat ggtcaaaacg gcgctgacgc tggcagtaac gaccacctta
     4021 aaggaattaa cgccgtgaaa atcgaactga cggtgaatgg actaaaggta caggcgcagt
     4081 actctgatga tgaaattgaa aatgtacata aaccgttgct gcgtatgctg gcggccttac
     4141 agacagtaaa tccgcagcgg cgtacggtgg tttttctttg cgctccgccg gggacgggta
     4201 aatctaccct aaccaccttc tgggaatatc tcgcacaaca agacccggaa ctgcccgcta
     4261 ttcagacact gccgatggat ggttttcacc attacaatag ctggctggat gcgcatcaat
     4321 tgagcccctt caaaggcgca ccagagacat tcaacgttgc gaaactggcg gaaaatctgt
     4381 gccgggttgt ggaaggggat tgtacgtggc cgcagtacga tcgacaaaag catgatcctg
     4441 ttgaagatgc gctgcacgtt accgcaccac tcgtcatcgt cgaaggaaac tggttgttgc
     4501 tggatgatga aaagtggtgc cagcttgcgc aattttgtga tttctcgatc tttatcaaag
     4561 ctcccgcatc tgcgttgcgg gagcgtctgg tggggcgcaa gctggcggga gggttatcgc
     4621 tggcagacgc agaagccttt tatgaccgta ccgacgggcc gaatgttcgc cgggtgctgg
     4681 aagagagtct gcctgcaaat ttgaccttaa tgatgaccgc tacgggtgaa tatcgtctga
     4741 tggattaacg ttcctgatta caccatcttc cagtcgcacc acacgagaga aatgacgccg
     4801 ggtaaatgcg gcgtcatgac tgattgctac gacgctcgtg cctcgctggc ggcacttttc
     4861 cagccagctt tcaaacacgc tcaaccaatt ttcgtcaaaa tcccgactcg gctcgtccag
     4921 tagcaatagt ggcggagaga gtgcttccag gcaggcgacg gcgaccatcc gtcgctgcgc
     4981 actatgcaga tctaacggat gcgcgctggc agtgtctgct aactgacagc attgcagggc
     5041 agcattggta cgttgagtga tttcatccgc cgggcatttt tgcagtatca aaccaaatgc
     5101 gatttcgtca gcaacggtgc tgtgaaaaag ctgattttcc gcttcctgaa acagtacgcc
     5161 aatctttgcc gcgcgctggc ggtttttcag gtttttcatc gcttgttgtt gcagcataac
     5221 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg attttcccgc
     5281 gccattgtcg ccggtcagcg ccagccattc accttgtttg agttgcagag aaatgtcgca
     5341 aagacaatcc gtagccgcgc caggccagcg ataagatatc tgatttaacg ttaacatagt
     5401 ggctggagcg ctccgttttg caattgccac gcttgctggc agtggttcag gaaaggtgtg
     5461 tgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa gcgcccagtg ttgtaagcgc
     5521 tgcaacagca tttcgcgggc ctgcggcgtc aggcggctaa aggcttcatc aagaattaag
     5581 agctttggct gcatcgcgat ggcgcaggcg atgaccacac gctgtgtctc tccaccggag
     5641 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagggccag ggcggcgtca
     5701 atccgcgcca taatctcttt ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     5761 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     5821 atggtggcgg attgttcatt aagcgggaga cgcccaatgg gcgtaccgag aagcgtcccc
     5881 gtgccggtta tctctcctgg tagaaagtcc ggataccagc ccgccatcag ctgcgccagc
     5941 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     6001 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aatagcgaaa ctgttctaac
     6061 gtaaccatat ccacgctccg aattcagcca gcattagcaa tatcatggcg taacgtgcta
     6121 cgcgttgtag ggtgctatcg gctggtgccc ataacgtagt gcgcctattg ttgattcgaa
     6181 atgcccgcat atccagcgcc gcaccacgga cagcaagatc gtttagcgca ttgtgtgtta
     6241 gcggaataat taacgcgggc atcgcacgta aacgctgata ccagccctca tccagcggca
     6301 cgccgcgggc gcgctgcgct tcatgaataa tcgccaattg ccgttttaac tgctcaacga
     6361 ccaataacgg cccggcaaac agataagcga cgcccggcgg caggcgagaa gcaaacaaag
     6421 cgcggataaa acgttgcacg gggacgtact gcatccacaa ctgtgaggtt gaaacaatcg
     6481 ccagaatccg caaccagagc gtaatagcat gcgcccaccg ttctggagag cgtggcgtgc
     6541 cgcttagcca ttctgtcagc cagccaccgt gcaccagcca cagtccagcc cccagcgaga
     6601 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     6661 aaaaggtcgc cgcgctatag actggcaata ttgtctgtgt gggcaggata agcgtggtgc
     6721 aggccgccag cgcccacagc gttaaggatg taaacggatg catttagcgc acggcagcca
     6781 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctgacg cagtaaaagc cagacaatca
     6841 ccgctgtgag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     6901 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg cttcctgtca
     6961 cgccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     7021 tcacgccgct aacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     7081 cggcgaccag gccgatgacc atcgcgacgg gcgcaaatgc ggcggcgatc gggtcggtca
     7141 gcaatcccca gagtaaattg gtcagtaaac cggttagcat cccgatcacc ggaccaagca
     7201 gtaccgcgct aataagtgtg ccgatggagt cgagaaaaat aggtaatttg accatgctgg
     7261 ccagttgccc gccgatcata ttgatggcga tagagataac gatcaacacc aatgcctgac
     7321 tggaaaagtg gcgacgcgcc ataagaaaac ctcactgaaa gtagttattt aacgggggga
     7381 aatatgcaac tcgtcgtaac cagaaagcgc gcaagactgg cggctaaacg ccagttcctc
     7441 aaggcagccg atcaccagtt caagcgtggt ccgcacggta cagccgggca tcatatgacc
     7501 gccaagcatc gtgccgtgtg gatcggaaac gcagagatga agatgttcac cactctgttc
     7561 cagcgtgccg ttcaatgcga taacttcgaa cttaccgctt agcagggcag tgttttcttg
     7621 cccggcatag cgtaaggcga cgtcagtcag actgccggta caacctgcaa tccacgcggc
     7681 gtggagttgt tgttgctgcg cgaaggcgcg taattgggag agaacttcct gcccggggag
     7741 caggcgcagc gcgtaaaaac gtgcggagga agaatatggg tagggcaatg tcatcattgg
     7801 agtactccga aactcggacg tattgcggat aatatcgacg ctgcatcagt catattctga
     7861 tataagtcag ggtttgtgac ggcacaacga aaagattttg ctacgcttat tcgtgattct
     7921 gcaaagtgga ataaattcca caggggcggg gctgaaaacg tgacgtaagt cagataattg
     7981 tcttctttga ctggacaatc attcctttta ttccacgttt cgcttatcct agctgaagcg
     8041 tttcagtcga ttaaatgttc gacaattaac caatcagtcg cagtttgcga caggtaaggt
     8101 ttccccgaac gatttgctgg attactctgt ctggcaaatt tgctcaggga aaaccttgca
     8161 ggagatctat gaccgtacgc gtagcgataa atggcttcgg tcgcatcggg cgtaatgtgg
     8221 ttcgtgcttt gtatgaatcc ggacgccgtg cggaaattac cgtggtggca atcaacgaac
     8281 tggcggatgc tgcgggcatg gcgcatttgt tgaaatatga caccagccat ggccgttttg
     8341 catgggaagt acgacaggaa cgcgatcaac tttttgttgg tgatgacgcc atccgtgtat
     8401 tgcatgaacg ttcactgcaa tcgctcccct ggcgtgaact tggcgttgat gtagtcctcg
     8461 actgcaccgg cgtatatggc tcccgcgagc atggtgaagc acacattgcc gccggggcta
     8521 aaaaagtgct cttttcacat cctggcagta acgatctcga cacgaccgtt gtttatggcg
     8581 tcaatcagga tcaacttcgt gcggaacacc gcatcgtttc taacgcttcc tgtaccacga
     8641 attgcataat tcccgtcatc aaattgttag atgatgcgta cggtattgag tccggcactg
     8701 tgaccacaat tcactccgcc atgcacgatc aacaggttat tgatgcatac catcctgacc
     8761 tgcgtcgcac tcgggcagcc agtcagtcga tcattccggt cgatactaaa ctggccgccg
     8821 gtatcacacg attttttccg caatttaacg atcgctttga agcgattgcg gtacgtgtgc
     8881 caaccataaa tgtgacggca atcgatttaa gcgtgacggt gaagaaacct gtaaaagcca
     8941 atgaagtcaa cctgttgctg caaaaagcag cacaaggtgc atttcatggt atagttgact
     9001 atacggaatt gccgttggtc tctgtagatt ttaaccacga tccgcacagt gccattgtcg
     9061 atggcactca aacccgggtc agtggcgcac acctgatcaa aacgttggtc tggtgcgata
     9121 acgaatgggg ctttgctaac cgaatgctcg acacgacgtt agctatggct actgttgctt
     9181 tcaggtaaga cgcaagcagc gtctgcaaaa cttttagaat caacgagagg attcaccatg
     9241 tctgtaatta agatgaccga tctggatctt gctgggaaac gtgtatttat ccgtgcggat
     9301 ctgaacgtac cagtaaaaga cgggaaagta accagcgacg cgcgtatccg tgcttctctg
     9361 ccgaccatcg aactggccct gaaacaaggc gcaaaagtga tggtaacttc ccacctgggt
     9421 cgtcctaccg aaggcgagta caacgaagaa ttctctctgc tgccggttgt taactacctg
     9481 aaagacaaac tgtctaaccc ggttcgtctg gttaaagatt acctcgacgg cgttgacgtt
     9541 gctgaaggtg aactggttgt tctggaaaac gttcgcttca acaaaggcga gaagaaagac
     9601 gacgaaaccc tgtccaaaaa atacgctgca ctgtgtgacg tgttcgtaat ggacgcattc
     9661 ggtactgctc accgcgcgca ggcttctact cacggtatcg gtaaattcgc tgacgttgcg
     9721 tgcgcaggcc cgctgctggc agctgaactg gacgcgctgg gtaaagcact gaaagaacct
     9781 gctcgcccga tggtggctat cgttggtggt tctaaagtat ctaccaaact gaccgttctg
     9841 gactccctgt ctaaaatcgc tgaccagctg atcgttggtg gtggtattgc taacaccttt
     9901 atcgcggcac aaggccacga tgtgggtaaa tccctgtacg aagctgacct ggttgacgaa
     9961 gctaaacgtc tgctgaccac ctgcaacatc ccggttccgt ctgatgttcg cgtagcaacc
    10021 gagttctctg aaactgcacc ggctaccctg aaatctgtta acgatgtgaa agctgacgag
    10081 cagatcctgg atatcggtga tgcttccgct caggaactgg ctgaaatcct gaagaatgcg
    10141 aaaaccattc tgtggaacgg tccggttggc gtgttcgaat tcccgaactt ccgcaaaggt
    10201 actgaaatcg tggctaacgc tatcgcagac agcgaagcgt tctccatcgc tggcggcggc
    10261 gacactctgg cagcaatcga cctgttcggc attgctgaca aaatctccta catctccact
    10321 ggcggcggcg cattcctcga attcgtagaa ggtaaagtac tgcctgcagt agcgatgctc
    10381 gaagagcgcg ctaagaagta aaaaatcaca gggcagggaa acctgccctt gtttcagcgc
    10441 gcttttagag cacgcacttt ttcatactct aaataattcg agttgcagga aggcgacaag
    10501 agagtgaatc cccaggagct tacataagta agtgattggg gtgagcgaac gcagacgcag
    10561 cacatgcaac ttgaagtatg acgagtataa ggcccgacga tacaggacaa gagacatgtc
    10621 taagattttt gatttcgtaa aacctggcgt aattactggt gatgacgtac agaaagtttt
    10681 ccaggtagca aaagaaaaca acttcgcact gccagcagta aactgcgtcg gtactgactc
    10741 catcaacgcc gtactggaaa ccgctgctaa agttaaagcg ccggttattg ttcagttctc
    10801 caacggtggt gcttctttta tcgctggtaa aggcgtgaaa tctgacgttc cgcagggcgc
    10861 tgctatcctg ggtgcgatct ctggtgcgca tcacgttcac cagatggctg aacattatgg
    10921 tgttccggtt atcctgcaca ccgaccactg cgcgaagaaa ctgctgccgt ggatcgacgg
    10981 tctgctggac gcgggtgaaa aacacttcgc tgctaccggt aagccgctgt tctcttctca
    11041 catgattgac ctgtctgaag aatctctgca agagaacatc gaaatctgct ctaaatacct
    11101 ggagcgcatg tccaaaatcg gcatgactct ggaaatcgaa ctgggttgca ccggtggtga
    11161 agaagacggc gtggacaaca gccacatgga cgcttctgca ctgtacaccc agccggaaga
    11221 cgttgattac gcatacaccg agctgagcaa aatcagcccg cgtttcacca tcgctgcgtc
    11281 cttcggtaac gtacacggtg tttacaagcc gggtaacgtg gttctgactc cgaccatcct
    11341 gcgtgattct caggaatatg tttctaagaa acacaacctg ccgcacaaca gcctgaactt
    11401 cgtattccac ggtggttccg gttctactgc tcaggaaatc aaagactccg taagctacgg
    11461 cgtagtgaaa atgaacatcg ataccgatac ccaatgggca acctgggaag gcgttctgaa
    11521 ctactacaaa gcgaacgaag cttatctgca gggtcagctg ggtaacccga aaggcgaaga
    11581 tcagccgaac aagaaatact acgatccgcg cgtatggctg cgtgccggtc agacttcgat
    11641 gatcgctcgt ctggaaaaag cattccagga actgaacgcg atcgacgttc tgtaagccat
    11701 tcctttctgc ttatctcaag gcccgctctg cgggtctttt tttcgccaaa agcaaggtga
    11761 tacagatgag aaatctgtga tctatttggc aaaattatgc tttattgttt acccttgtca
    11821 gactgcccgt cataaggcgg cggagtgtat ttctccattt tgagtcagtt gaaaaggaat
    11881 attgaatgga agatttgaat gttgtcgata gcataaacgg cgcgggaagc tggctggtgg
    11941 ctaaccaggc gctgctgcta agttatgcag taaacatcgt ggcggcactc gcgatcatca
    12001 tcgttggttt gattatcgcg cggatgattt ccaacgcggt gaatcgcctg atgatctccc
    12061 gtaaaatcga tgccactgtt gctgattttc tctctgcatt agtccgttac ggtattatcg
    12121 cctttacgct aatcgctgca ctgggccgcg tgggcgtaca aacagcgtca gtcattgctg
    12181 tactcggtgc cgcaggctta gcggttggtc tggctttgca ggggtcactg tctaacctgg
    12241 ccgctggcgt gttacttgtc atgttccgcc cgttccgtgc cggagaatat gttgacctcg
    12301 gcggcgtagc cggtactgtg ctgagtgtgc agattttctc caccaccatg cgtactgcag
    12361 acggtaaaat tatcgttatt ccgaacggta aaattattgc cggaaatatt attaacttct
    12421 cccgcgagcc agttcgccgt aacgaattta ttattggcgt ggcgtatgat tccgatatcg
    12481 atcaggttaa gcagatcctg accaatatta tccagtctga agatcgcatt ttgaaagatc
    12541 gcgaaatgac tgtgcgcctg aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg
    12601 tctggagcaa cagcggcgat ctgcaaaacg tgtactggga tgtgctggag cgtattaaac
    12661 gtgaatttga tgccgccggt atcagcttcc cgtacccgca aatggatgtg aactttaagc
    12721 gggtgaaaga agacaaagct gtgtaatcaa cgctgaggcc agataatact ctctggcctc
    12781 tctcttatta gtttttctga ttgccaatta atattatcaa tttccgctaa taacaatccc
    12841 gcgatatagt ctctgcatca gatacttaat tcggaatatc caacgtgttt tcttattact
    12901 ttcaaggtct tgtgcttggc gcagctatga tcctaccgct cggtccacaa aatgcttttg
    12961 tgatgaatca gggcattcgt cgtcagtacc acattatgat tgccttactt tgcgctatca
    13021 gcgatttggt cctgatttgc gcggggattt ttggtggcag cgcgttattg atgcagtcgc
    13081 cgtggttgct ggcgctggtc acctggggcg gcgtagcctt cttgctgtgg tatggttttg
    13141 gcgcttttaa aacagcgatg agcagtaata ttgagttagc cagtgccgaa gtcctgaagc
    13201 aaggcagatg gaaaattatc gccaccatgc tggcagtgac ctggctgaat ccgcatgttt
    13261 acctggatac ttttgttgta ctgggcagcc ttggcgggca acttgatgtg gaaccaaaac
    13321 gttggtttgc gctcgggaca attagcgcct ctttcctgtg gttctttggt ctggctattc
    13381 tcgcagcctg gctggcaccg cgcctgcgca cggcaaaatc acaacgcatt atcaatctgg
    13441 tagtgggatg tgttatgtgg tttattgcct tgcagctggc gagagacggt attgctcatg
    13501 cacaagcctt gttcagttag gcgctatctg atggaaaaat aaaacagagg cgctaagctt
    13561 gcctccagag gtcctgaatt ttgggcaagt caattaacac ggagagacta acgtgaagtt
    13621 caaagttatc gccctggcgg cattaatggg tattagcggg atggcagcgc aggctaacga
    13681 attgccggat ggaccgcata ttgtcacctc cggtacggca agcgtggatg cggtgccaga
    13741 cattgccact cttgcgattg aagtcaacgt ggccgcgaag gatgccgcta ctgccaagaa
    13801 acaggcggat gagcgcgtcg cacaatacat ttccttcctt gaactcaatc agatcgcgaa
    13861 aaaagatatc agctcagcga acttacgcac ccagccagat tatgattatc aggatggtaa
    13921 aagtatcctg aaaggctacc gtgctgtgag aacggtggaa gtcacgctcc gtcagttaga
    13981 caaactgaat tccttgctgg atggcgcgct gaaggcgggt cttaacgaaa ttcgttctgt
    14041 atcgctgggc gtggcgcagc cggatgtcta taaagacaaa gcgcgtaagg cagcgattga
    14101 taacgcgatt catcaggcgc aggaactggc gaacggcttt catcgtaaac tggggccggt
    14161 atatagcgtg cgctaccatg tttccaacta tcagcccagc ccaatggtgc ggatgatgaa
    14221 agccgatgcc gcgccggtgt ccgcccagga aacttacgag caggccgcta ttcagtttga
    14281 tgatcaggtc gatgtggtct tccagttaga acctgtggat caacaacccg ctaaaacacc
    14341 tgcagcacaa taatatttgt agcccgacgt attcgtcggg ctaatgcctt tacccgatat
    14401 tgcctccttt ttctgtaatt ttctgtaaaa ccatgacgta atcacctatc tttgctcgcg
    14461 gtttcatcaa ggataggctg actgaaacgg ttaagcggac aaggatgcgg gggagggggt
    14521 atggacattt ttatttcgaa aaagatgcgc aatttcattt tattagcgca aaccaataat
    14581 attgcccggg cggcagaaaa aatccatatg acggcttcgc gatttggcaa aagcattgcc
    14641 gcgctggaag aacaaattgg ctatacgcta tttacccgca aagataataa tatcagcctc
    14701 aataaagccg gacaggaact gtatcaaaaa ctgttcccgg tttatcaacg actttctgcg
    14761 attgataatg aaatccataa ctcaggacgt cgttcacgag acattgtgat aggcatagat
    14821 aatacttatc cgaccattat ttttgatcag ttgattagtc tcggagataa gtacgaaggc
    14881 gtgaccatcc agccagttga gttcagcgaa aatggcgtta tcgataatct ttttgaccgt
    14941 cagttagatt ttataatctc cccacagcat gtatctgcac gagttcagga actggaaaaa
    15001 ctgactatca gtgaactacc accgctgcga ttgggctttc acgtttctcg ccgttatgaa
    15061 gggcgacagg agcaagagct gcttcaggag ctgccctggc tacaaatgcg ctttcaaaac
    15121 cgggctaact tcgaagcaat gattgatgcg aatatgcgtc cttgtgggat caatccaacc
    15181 attatttatc gtccctatag ttttatggcc aaaatcagcg ccgtggagcg gggacatttt
    15241 ctgacggtga tcccgcattt tgcctggcga ctggtgaatc cggcaacgct gaaatatttt
    15301 gatgcgccgc acaggccgat gtatatgcag gaatatcttt attcaatcag aaatcatcgt
    15361 tataccgcca cgatgcttca gcatattgct gaagatcgtg acgggacaaa ccattaaccc
    15421 agcatcgatc cggttgcaat taaattacgg tgtaagtcga agacgtggct aagatcgtgg
    15481 tgaatatgtc cgccggacgc attttccaga tagcgatgca gataatcccg acacatagga
    15541 tgtgcacaat gatcaatggt ggtgcggata acgttgttat acggatcctg tgcgccaaat
    15601 ccactgaccg cttcgctcca taaataacgt gccgcacgca acatggcggc gttcataaac
    15661 agatccatgc caatgccgaa gaaaaacgac aggcgaggga cgaagccatc aattttcagc
    15721 ccggcggaga ttgctgcttt gatgtactca atcctatcgg cgagcgtaaa tgctacctgc
    15781 tgcacgcagt tggcacccgc ttcacccatg tggtaactgc tgatactgat ggtattaaat
    15841 cgcggcatgt tgccggaaca ccaggcgatg atgtctgcga taatacgcat tgacggtttt
    15901 ggcgggtaaa tataggtatt gcggcagagg tactctttga ggatatcgtt ctgaatggtg
    15961 ccggtcagtt tatcggatgt aacaccttgc tcttctgcgg cgacgatata aaacgccagt
    16021 actggtagca ctgcgccatt catggtcata gaaaccgaca ttttatccag cgggatctgg
    16081 tcgaacagga ctttcatatc ttccacggtg tcgatagcga cgcccgcttt gccgacgtcg
    16141 cccgccacgc gtgggttatc ggagtcgtag cctcggtggg cggcaaggtc aaacgcaacg
    16201 gaaagacctt tttgcccggc ggccaggtta cggcgataaa aagcgttgga ctcttttgct
    16261 gtcgagaaac cagcatactg acggatggtc cacggttggg tggtatacat agtggcacgc
    16321 gggccacgaa cgtagggtgg caaaccagga agagtacccg tcacctccag attatcgaga
    16381 tcggcttcgg tatacagcgg cttgatagcg atcccttccg cggtttgctg aaccagcgag
    16441 tcgacagttt tctcccgacg gctcaattcc ttgttggcaa gctgttgcca ctcctgcacg
    16501 ttagacatta tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc ttacggtgag
    16561 ctttttacgt gggtttgtcg ccagcaaaaa aatgcattca gacgtcgttt tttgccgcct
    16621 gagatttgtc tattttgtga tgtgattcaa ggaatacaaa aagaagtgag gataacgcct
    16681 gatatgtgct atcaggcgtt atttgatgga ttaatcctga cgaagaactt tgtgaccata
    16741 atcgagcaac gcatcagtga ctttacgcat catgcggctt tcaggagcaa agcggtgcca
    16801 gtagagcatc cggcgttgaa atagccctgg cgttaagtca atcagttcgc cgctggccag
    16861 ctctttctcg atttgcaggt gcgggatcat acagcaggtg gtgccctgac gagcaagttg
    16921 cacgaacgct tccgaagaat taacgatatg gcagggtacg ctgccaggag gcagatcgaa
    16981 attttgctgc aaaaaggcct ggtgcatatc gtcaagatgg tcaaacgcga ccactggcgc
    17041 tttcagtaat gccgaacgcg ttacgccgtt agggaaatat ttttcggcaa acggttttga
    17101 gctgacgaac agatagtcga gcgcaccgag tttatcgaca agacaactcg gcagcgcctg
    17161 atgttgaata ctcactgcgc cgaccacttc gccgcgacgc agacgttcct gagtgcgggt
    17221 ttcatcttct acctgcaagt tgaggcggat gggcgaatca gccaacacag gagccagtgc
    17281 cggaagcaac cacgtcgcca gactgtcggc gttgaccgcc agtgaaagca gcagcggagt
    17341 cgaaccggtt tgttcatcgc ccagccactc ttcttccagc agctccacct ggcgtagtag
    17401 cgccaacagt ttttgccctt gttccgtcgg gcgcggcggt acggtacgca ctaacagcgg
    17461 ctgcccgaac atattttcca gttgcttaat gcgctgtgag acggctgatt gtgtaatgca
    17521 cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc accgcatcca gtgcctgtaa
    17581 tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc tggtaaatgc tttttcctgc
    17641 actatgacac aattttgtgt cgggttgcat ataccgaagc cgttggcggg taacatttgt
    17701 atcagtggtt aacgcgcagc ttaaaagatc agggatttgc gctaatgcat ttcgctcagc
    17761 gagttcgcgc cctggttgtg ttgaatggcg tggcgttatt acctcagttt gcctgtaaac
    17821 aggggcttgc gaacggggaa ctggtgcgcc tgtttgcacc gtggagcggc atacccagac
    17881 cgttgtatgc tttatttgcg gggcgaaagg ggatgcccgc cattgcgcga tattttatgg
    17941 atgagttaac cacgcggctt gccaacgggg tctgaatcgc tttttttgta tataatgcgt
    18001 gtgaaatttc ataccacagg cgaaacgatc atgacgcagg atgaattgaa aaaagcagta
    18061 ggatgggcgg cacttcagta tgttcagccc ggcaccattg ttggtgtagg tacaggttcc
    18121 accgccgcac actttattga cgcactcggt acaatgaaag gccagattga aggggccgtt
    18181 tccagttcag atgcttccac tgaaaaactg aaaagcctcg gcattcacgt ttttgatctc
    18241 aacgaagtcg acagccttgg catctacgtt gatggcgcag atgaaatcaa cggccacatg
    18301 caaatgatca aaggcggcgg cgcggcgctg acccgtgaaa aaatcattgc ttcggttgcg
    18361 gaaaaattta tctgtattgc agacgcttcc aagcaggttg atattctggg caaattcccg
    18421 ctgccagtag aagttatccc gatggcacgt agtgcagtgg cgcgccagct ggtgaaactg
    18481 ggcggtcgtc cggaataccg tcagggcgta gtgaccgata acggcaacgt gatcctcgac
    18541 gtccacggca tggaaatcct tgacccgata gcgatggaaa acgccataaa tgcgattcct
    18601 ggtgtggtga ctgttggttt gtttgctaac cgtggcgcgg acgttgcgct gattggcaca
    18661 cctgacggtg tcaaaactat tgtgaaatga tctgacgggg gaacctcccc cgttaaaaaa
    18721 attctcttca ttaaatttgg tgacatgtgt cacgttttta cctggcaatt gtcgattgct
    18781 ctaaataaat cttctaaacc agcatattca tccaagaaat acttttgcgt gatatttcct
    18841 caacatcgcg acgcaaacgt tcatattgcc gcaatattat tttttgatat gttgaaaggc
    18901 ggatgcaaat ccgcacacaa catttcaaaa gacaggattg ggtaaatggc aaaggtatcg
    18961 ctggagaaag acaagattaa gtttctgctg gtagaaggcg tgcaccaaaa ggcgctggaa
    19021 agccttcgtg cagctggtta caccaacatc gaatttcaca aaggcgcgct ggatgatgaa
    19081 caattaaaag aatccatccg cgatgcccac ttcatcggcc tgcgatcccg tacccatctg
    19141 actgaagacg tgatcaacgc cgcagaaaaa ctggtcgcta ttggctgttt ctgtatcgga
    19201 acaaaccagg ttgatctgga tgcggcggca aagcgcggga tcccggtatt taacgcaccg
    19261 ttctcaaata cgcgctctgt tgcggagctg gtgattggcg aactgctgct gctattgcgc
    19321 ggcgtgccgg aagccaatgc taaagcgcat cgtggcgtgt ggaacaaact ggcggcgggt
    19381 tcttttgaag cgcgcggcaa aaagctgggt atcatcggct acggtcatat tggtacgcaa
    19441 ttgggcattc tggctgaatc gctgggaatg tatgtttact tttatgatat tgaaaacaaa
    19501 ctgccgctgg gcaacgccac tcaggtacag catctttctg acctgctgaa tatgagcgat
    19561 gtggtgagtc tgcatgtacc agagaatccg tccaccaaaa atatgatggg cgcgaaagag
    19621 atttcgctaa tgaaacccgg ctcgctgctg attaatgctt cgcgcggtac tgtggtggat
    19681 attccagcgc tgtgtgacgc gctggcgagc aaacatctgg cgggggcggc aatcgacgta
    19741 ttcccgacgg aaccggcgac caatagcgat ccatttacct ctccgctgtg tgaattcgac
    19801 aatgtccttc tgacgccaca cattggcggt tcgactcagg aagcgcagga gaatattggc
    19861 ctggaagttg cgggtaaatt gatcaagtat tctgacaatg gttcaacgct ctctgcggtg
    19921 aacttcccgg aagtctcgct gccactgcac ggtggtcgtc gtctgatgca catccacgaa
    19981 aaccgtccgg gtgtgctgac tgcgctcaac aaaatttttg ccgagcaggg cgtcaacatc
    20041 gccgcgcaat atctgcaaac ttccgcccag atgggttatg tcgttattga tattgaagcc
    20101 gacgaagacg ttgccgaaaa agcgctgcag gcaatgaaag ctattccggg taccattcgc
    20161 gctcgtctgc tgtactaaat ccccttctct gaaaatcaac gggcaggtca ctgacttgcc
    20221 cgttttttta tcccttctcc acaccgttgc cgcattgcgc attctacttt gcgagtctgc
    20281 tccagaaaat aaatcaaagt cagatcaatg cgttgtgttt atttaatggg gatttacgat
    20341 ggcagggcag catggggctg taacggtaaa gccctcaccg aagcgagggc ttgaaggaga
    20401 agggttatga tgcgacttgt catcatactg attgtactgt tactcataag tttcccagct
    20461 tattaacagt cagtctcagg ggaggagtca tcctccctta cccttactca ctaaattagg
    20521 tcaaagaatc aacgatgtca atcagggcga tgcgggatgt atcgccctta ccattcccag
    20581 actttcgacg gtgtaaccac cgcaggcagt ggaatatccc atttctccac tgggagtttt
    20641 tctactaact ggcaatcatg cgcataaccc accggttgca ttttatagtg ctgccagttt
    20701 tgtaaggtcc gatcataaaa accaccgccc attcccaggc gctgaccgta ctcatcaaag
    20761 gcgaccagcg gtgttatcaa cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    20821 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtagttc
    20881 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    20941 gcgcgccaga gttgttctat gagtggctgg gtgtcgagtt cgccatcaaa agagaggaat
    21001 acagcgacca tatgtgccat caccaccgga ggataagtca tcatccgggt agcggcttgt
    21061 tgacccattt cctgctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    21121 cggatttctt gtcgggataa tgtcagtgga agttctggta gttgcgtcat ggctggtgcc
    21181 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    21241 aggtgaatgt gtcgtcgtag ttttaaggct tctcggacgg accgagcatg ctcaccaacc
    21301 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    21361 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    21421 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    21481 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtcttcgctt
    21541 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    21601 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    21661 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    21721 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    21781 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    21841 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    21901 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    21961 ctgaaccaac aagggacggg tctgacccct gctgagatgc atggtttaat cagtgggatg
    22021 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    22081 ggcatggctt tcggtcatga gctggcacag gcactgcgca aaatgcactc tgccaccagc
    22141 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    22201 agcgttttcg atcgggctga tgcgctggct ggttgggtca atcacttcct gcttggtctt
    22261 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    22321 ctgcgtaaca tcgcgcagtt gggttacgac gaagacgaag atcaggaaga gcttgaaatg
    22381 tcgcttgaag agatcatcga gtacgtccgt gttgccgcgc tgttatgcca cgacaccttt
    22441 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    22501 atgtaaggag agtgttatga gtgatatatc ccggcaagag tttcagcgtc gccgtcaggc
    22561 cctggtagag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    22621 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    22681 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    22741 cgttttgttt aaccgcgttc gcgacctgac ggcagagatc tggtttggtc gtcgcttagg
    22801 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    22861 tcagcaactt tatcaactac ttaatggtct ggatgtggtt taccacgccc agggcgaata
    22921 tgcatatgct gatgaaatcg tgaacagcgc gcttgagaaa ctgcgtaaag gttcgcggca
    22981 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    23041 gttcaaatca ccagaagaga ttgccgtact tcgccgcgcg ggtgaaatca ccgcactggc
    23101 gcatacacgg gcgatggaaa aatgccgtcc ggggatgttc gagtaccatc tggaaggcga
    23161 aattcaccac gaatttaacc gccacggtgc gcgttaccct tcctataaca ccattgttgg
    23221 cagcggtgaa aacggctgca ttttgcacta caccgaaaac gagtgtgaac tgcgtgacgg
    23281 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgctggcg acattacccg
    23341 caccttcccg gtcaacggca aattcactca ggctcagcgt gaaatctatg acattgtgct
    23401 ggagtctctt gaaaccagcc tgcgtctgta tcgtccggga acttccattc aggaagtcac
    23461 tggtgaagtg gtgcgcatca tggttagcgg tctggtaaaa ctcggtatcc tgaaaggtga
    23521 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    23581 ctggttagga ctggatgttc atgacgttgg tgtttatggt caggatcgct cgcgtattct
    23641 ggaaccgggt atggttctga ccgtagaacc agggctgtat attgcgccgg atgcagaagt
    23701 gccagaacag tatcgcggta tcggcattcg tattgaagac gatattgtga ttaccgaaac
    23761 cggtaatgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    23821 ggcagctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    23881 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    23941 accgcgccag agtcacatgc tcatccgggc tttgatggac gagcgattgc gctggcagca
    24001 ggcacttgtc agcaactggc gcgcatcggt gtctggcaat ctctggccga ttgcgcaacc
    24061 gccatcacca ccgttcatgt cagcgatcgc ggccatgccg gattcgtcac tcttgccgca
    24121 gaagattacc aactggcggc gctagggcag gttgtcgaat tgcacaatgt tgggcaacgg
    24181 ctgtttgcgc tgttgcgtaa agcaccaggc gtcacgctgc attgccctga tcgcgtggct
    24241 aacgtttccc gtactcagag tcacgttgaa gtgacgctgg agagtggcgg gacgctgacg
    24301 ggccgcgtgc tggtagccgc tgatggcacc cattcagcgt tagccactgc gtgcggtgtt
    24361 gactggcagc aagagcctta cgagcaactg gcagtgattg cgaacgttgc gacttccgtt
    24421 gcgcacgaag gccgcgcttt tgaacgcttt acgaaacatg gcccgctggc gatgttgccg
    24481 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    24541 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt cggcctttgg ctggcgactt
    24601 gggaaaatta ctcacgccgg aaaacgtagt gcttatccgc tgacgttaac ccgtaccgcc
    24661 agacctatta cccatcgtac cgtgctggtg gggaatgcgg cgcaaactct gcaccccatc
    24721 gccgggcaag gatttaacct cggtatgcgt gatgtcatga gccttgcaga aaccctgact
    24781 caggcgcagg agcgcggaga agacatgggt gattacggcg tattgtgccg ttatcagcag
    24841 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagccttgt acatcttttt
    24901 gccaaccgtt ggacgccgct ggttgtcggg cgcaacatcg ggctgatgac gatggaatta
    24961 tttaccccgg cacgcgatgt gctggcgcag cgcactcttg gttgggtggc gcgttgaagg
    25021 cggttctaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    25081 cgggctggcg gttgcctgcg gcttacaggg tagcggctta cgcgttgccg tactggagca
    25141 gcgcgtaccg gaacctctgg cggcggatgc accaccacaa ctgcgcgttt cggctatcaa
    25201 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caagacattc tctctcgtag
    25261 ggcctgttgc tatcacggta tggaagtgtg ggataaagac agttttggtc acatctcgtt
    25321 tgacgatcaa agcatgggct atagccatct tgggcatatc gttgaaaatt cagtgattca
    25381 ctacgcgctg tggaacaaag cccggcagtc gtcagatatc actctgttgg cccccgcaga
    25441 attacagcag gtcgcttggg gagaaaatga aaccttcctg acgttgaaag atggcagcat
    25501 gttaacggcg cgtctggtga ttggcgcaga cggcgctaat tcctggttgc gcaacaaagc
    25561 cgatattccg ctgactttct gggattatca gcatcacgcg ctggtggcga ccattcgcac
    25621 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    25681 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcattgtcgc cagaggaagc
    25741 gcagcggatg cagcaggcta gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    25801 taatcgcctg ggcttatgca aggttgagag cgagcgtcag gtgttcccgc tgacggggcg
    25861 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgctg cgcatactat
    25921 tcacccgctg gcggggcagg gggtaaatct cggctttatg gatgctgcag agctgattgc
    25981 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgtcg
    26041 ctatgagcgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggattccg
    26101 cgatctgttt tccggggcta atccggcgaa aaaattgctg cgtgatattg gtctgaaact
    26161 ggctgatacg cttcctggcg ttaagccaca acttattcgt caggcaatgg gattaaacga
    26221 tttacctgaa tggctgcgtt aaatatttct cttctgctgt ttatttgata cccatcacac
    26281 tttcatctcc cggtcttttc gccgggggat tttcctcatt tgaaataaac taatttcacc
    26341 tgcgatttcg cattatattt tctaatgcca cgaatttttg atttagtgtt ttttgacatt
    26401 tttttagttc ttaatgttgt cttattcaaa ttaactttct catctattta tctttatata
    26461 gagatttgtt cattttttgg ttttttattc tgttacgatt ttggcgtttt ttaaccataa
    26521 gctaatgtga tgatcaattt aaccttatgg ttaacagtct gtttcggtgg taagttcagg
    26581 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt tcgtggtgat
    26641 aatttcgcca tggaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    26701 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    26761 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    26821 cggaatgttt gatgtgtcac atatgaccat cgtcgatctt cgcggcagcc gcacccggga
    26881 gtttctacgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    26941 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    27001 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    27061 gattacccaa cacgcagaac ctttcggcat cgaaattact gttcgtgatg acctttccat
    27121 gatcgccgta caagggccga atgcgcaggc aaaagctgcc acactgttta atgacgccca
    27181 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggcgggcg atctgtttat
    27241 tgccaccacc ggttacaccg gtgaagcggg ctacgaaatt gcgctgccca atgaaaaagc
    27301 ggccgatttc tggcgcgcgc tggtggaagc tggcgttaag ccatgtggcc tgggcgcgcg
    27361 tgacacgctg cgtctggaag cgggcatgaa tttgtatagc caggagatgg acgaaactat
    27421 ttctccttta gccgccaata tgggctggac tatcgcctgg gaaccggcag atcgtgactt
    27481 tatcggtcgt gaagctctgg aagcgcagcg tgaacatggc acagaaaaac tggttggtct
    27541 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgtttta ctgatgcgca
    27601 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccaacgc tgggttatag
    27661 cattgcgctg gcgcgcgtgc cggaaggtat tggtgaaacg gcgattgtgc aaattcgcaa
    27721 ccgtgaaatg ccggttaaag tgacgaaacc tgtttttgtg cgtaacggca aagccgtcgc
    27781 gtgatttact tttttggaga ttgattgatg agcaacatac cagcagaact gaaatacagc
    27841 aaagaacacg aatggttgcg taaagaagcc gacggcactt acaccgttgg catcaccgaa
    27901 cacgctcagg agctgttagg cgatatggtg tttgttgacc taccggaagt gggcgcaacg
    27961 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    28021 gcgccagtaa gcggtgaaat cgtggcggta aacgatgcac tgagcgattc tccggaactg
    28081 gtgaacagcg aaccgtatgc aggtggctgg attttcaaaa tcaaagccag cgatgaaagc
    28141 gaactggaat cactgctgga tgcaaccgca tacgaagcat tgttagaaga cgagtaacgg
    28201 ctttattcct cttctgcggg agaggatcag ggtgaggaaa tttacgcctc accctcactc
    28261 tcttcgtaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc gctcatgaca
    28321 cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat cggaccggac
    28381 gccgcgcaac agcaagaaat gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc
    28441 ggccagattg tgccgaaaga tattcagctt gcgacaccac cgcaggttgg cgcaccggcg
    28501 accgaatacg ccgcactggc agaactcaag gctattgcca gtcgcaataa acgcttcacg
    28561 tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttatcct gcgtaacatg
    28621 ctggaaaatc cgggctggta taccgcgtat actccgtatc aacctgaagt ctcccagggc
    28681 cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttaaccgg gctggatatg
    28741 gcctctgctt ctcttcttga cgaagccacc gctgccgccg aagcaatggc gatggcgaaa
    28801 cgcgtcagca aactgaaaaa tgccaaccgc ttctttgttg cttctgacgt tcatccgcaa
    28861 acgctggatg tggtccgtac tcgtgccgaa acctttggtt ttgaagtgat tgtcgatgac
    28921 gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca ggtcggcact
    28981 actggtgaaa ttcacgacta caccgcgctt atcagcgaac tgaaatcacg caaaatcgtg
    29041 gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa ctgcgccggg taaacagggc
    29101 gcggatattg tttttggttc ggcgcaacgc tttggcgtac cgatgggcta tggtggccca
    29161 cacgcggcat tctttgcggc gaaagacgaa tacaaacgct caatgccggg ccgtattatc
    29221 ggtgtatcga aagatgcagc tggcaacacc gctctgcgca tggcgatgca gactcgcgag
    29281 caacatattc gtcgtgagaa agcgaactcc aacatttgta cttcccaggt actgctggca
    29341 aacattgcca gcctgtatgc cgtttatcac ggtccggttg gcctgaaacg tatcgctaat
    29401 cgcatccacc gtctgaccga tatcctggcg gcgggcctgc aacaaaaagg tctgaagctg
    29461 cgccatgcgc actatttcga tactttgtgt gtggaagtgg ccgacaaagc gggcgtgctg
    29521 gcgcgtgccg aagcggctga aatcaacctg cgtagcgata ttctgaacgc ggttgggatc
    29581 acccttgatg aaacaaccac gcgcgaaaac gtgatgcagc ttttcagcgt gctgttgggc
    29641 gataaccacg ggctggacat cgacacactg gacaaagacg tggctcacga cagtcgctct
    29701 attcaggctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt taatcgctac
    29761 cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga tctggcgctg
    29821 aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc cgccgagatg
    29881 atcccgatca cctggccgga atttgccgaa ctgcacccgt tctgcccgcc ggaacaggcc
    29941 gaaggttatc agcagatgat tgcgcagttg gctgactggc tggtgaaact gaccggttac
    30001 gacgccgttt gtatgcagcc gaactctggc gcacagggcg aatacgcggg cctgctggcg
    30061 attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg atatctgcct gatcccggct
    30121 tctgcgcacg gcactaaccc ggcttccgcg catatggcag gaatgcaggt ggtggttgtg
    30181 gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc ggaacaggcg
    30241 ggcgataacc tctcctgtat catggtgacc tatccttcta cccacggcgt gtatgaagaa
    30301 acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta ccttgatggc
    30361 gcgaacatga acgctcaggt tggcatcacc tcgccgggct ttattggtgc ggacgtttcc
    30421 caccttaacc tgcataaaac tttctgcatt ccgcatggcg gtggtggtcc gggtatggga
    30481 ccgatcggtg tgaaagcgca tctggctccg tttgtaccgg gtcatagcgt ggtgcaaatc
    30541 gaaggcatgt taacccgtca gggcgcggtt tctgcggcac cgttcggtag tgcctctatc
    30601 ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa aaaagcaagc
    30661 caggtggcaa ttcttaacgc caactatatt gccagccgtc tgcaggatgc cttcccggtg
    30721 ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg
    30781 aaagaagaaa ccggcatcag cgagctggat attgccaagc gcctgatcga ctacggtttc
    30841 cacgcgccga ccatgtcgtt cccggtggcg ggtacgctga tggttgaacc gactgaatct
    30901 gaaagcaaag tggaactgga tcgctttatc gatgcgatgc tggctatccg cgcagaaatt
    30961 gatcaggtga aagccggtgt ctggccgctg gaagataacc cgctggtgaa tgcgccgcac
    31021 attcaaagcg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt tgcagtattc
    31081 ccggcaggtg tggcagacaa atactggccg accgtgaaac gtctggatga tgtttacggc
    31141 gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt tactgattcg
    31201 actatcttat aaaggcgctt cggcgccttt ttcgcttgtt aataaagtgc gctttgtgtg
    31261 tttcggctga ggcgtaatat aaaatattgc gagctcaata tattaaaaga gtgccaggcc
    31321 tgatgagcgg agcacatcag gcacatcggc taattacaga ttctcgccgt tgctggcaat
    31381 cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc cagtaccgtc
    31441 gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg tggtgaacga
    31501 cacacagtcg atgcagcccc acggcgtgta gcccatcagg tccacgccat cgtaagtcac
    31561 cgcttttttc atctcttcaa tatgggcacg caggtaatca atgcggtaat cgtcattgat
    31621 gctgccatca tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg
    31681 cttctggtaa cgctcataca gctcgcaaag tgcgtagcgc agaccaaccg gatcaatctg
    31741 ccagccccag tcagacgctt taacatacgg attcggtacg ctgccttcga aaccagaaat
    31801 cgcatcgccg gtgccacctt cggccttcac cgcgttggtc atgtaatagc tgaagccgag
    31861 ataatcgcag gtgccttcgc gcagtacatc cagatcgccg tcttccattt tgatgttaaa
    31921 tccgcggcgt tcccactcgt tcaacacata ggacgggtaa tagccacgca actgcacatc
    31981 ggtaaagacg taacgttcgc gcatcgactc ctgggcaaac atcacatcgt ccgggttaca
    32041 ggagtaagga tagagcggca ccatcgccag catacaaccg actttcatct ccgggttgat
    32101 gcgatgcgcg gctttcaccg ccagggcgct ggcgacaaac tggtgatgca gcacctgata
    32161 catcgtctct tccgggtttt catgctcggt atacaccacg ccggagcagc agtaaccgaa
    32221 cagcggtgca cgccagttac gctggttgtt aatttcgttg aaggtcatcc agtatttgac
    32281 tttatgctta tagcgttcaa ataccacttc cgcgaaacgg acaaagaaat caaccacttt
    32341 acggttggtc cagctaccat attgctgcac cagatgcagc ggcatttcaa agtgggagag
    32401 ggtgatcacc ggttcgatgt tgtatttcag cagttcatcg atcatatcat cgtagaactt
    32461 cagcccttct tcgtttggct gagcttcatc gccttttgga aaaatgcgcg tccaggcgat
    32521 ggaagtacga aaacatttga agcccatttc ggcaaatagc ttgatgtcct ccttgtagtg
    32581 accataaaaa tcaacggctt catggtttgg atagtatttg ccaggcacaa cttctttggt
    32641 gatttcgcgc ggcacgccgt gtgcgccacc ggtcaaaacg tcacaaatgc tcggcccttt
    32701 gccgcctttg ttccagccgc cttcgacctg atgagcggca actgcgccgc cccataagaa
    32761 atctttcggt aaggtgagtt ttttcactat cataggctcc agatgagtca attcttacag
    32821 gaaatattac catgcagcca aacgacatca ctttttttca acgtttccag gatgacattc
    32881 tggctgggcg taaaaccatc accatccgcg acgagtctga atcgcacttc aaaacgggtg
    32941 atgtgcttcg tgtcggacgt tttgaagatg acggttattt ttgcacgatt gaagtcaccg
    33001 caacctcaac cgtaacgctg gatacgctga cggaaaaaca tgcagagcag gaaaatatga
    33061 ccctgactga actgaaaaag gtcattgccg acatctatcc cgaccagaca cagttttatg
    33121 tgattgaatt taaatgtctt taaattgaac tcacgcctgt tagttgaaat ttcgcattta
    33181 atttcatgat ttttcggaga ttaatctcaa atcagaattt attttagcta acaggtgttc
    33241 actggaacta ttctcagtta cgctggagag gtatacatgt gcgtgtacgt ttttccggag
    33301 taagttatgg ttcagaagcc cctcattaag cagggatatt cgctggcaga ggaaattgcc
    33361 aacagcgtca gtcacggcat tgggctggtg tttggtatcg ttgggctggt gttgctactg
    33421 gttcaggcgg tggatctcaa tgccagcgcc acggcgataa ccagttacag cctctatggc
    33481 ggcagtatga tcctgctgtt cctcgcttcg acgctctatc acgccattcc ccatcaacgg
    33541 gcaaaaatgt ggctgaagaa atttgaccac tgtgccattt atctgttgat tgccggaacc
    33601 tatacgccgt ttttgctggt ggggctggat tctccgttag cgcgcgggtt gatgattgtt
    33661 atctggagcc tggcattgct gggtattctg tttaaactga ccatcgcgca ccgattcaaa
    33721 attttatctc tggtgaccta tctggcgatg ggctggctgt cgctggtggt aatttatgaa
    33781 atggcagtta agctcgcggc gggcagcgtt accttactgg cggtaggcgg tgtggtttat
    33841 tcgctcgggg tgattttcta cgtctgcaag cgcattccat acaatcatgc catctggcac
    33901 ggctttgtgc tcggcggtag tgtgtgccac tttctggcga tctatttgta tattgggcag
    33961 gcgtaattgg cgtgcctttg taggcctgaa aagacacacc agtgtcgcat caggcgataa
    34021 atgcctgatg cgatacagag atttactctt ccagcgaata tggcagtggc tcgatacgca
    34081 atgtattcgc atcgtcacgc acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    34141 caacctgcac cacaacctga ccatcttcca gtttgaccgc agccagcacc gtaccggtac
    34201 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    34261 cgctaccctt cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    34321 ccatctcttg tccggtatag cagcctttct taaagctgat accgcccagc gcctggaggt
    34381 tggtcgcctg ggggataaac tgcccgctgt tggcggcatc aatcaccggg aaacccgctt
    34441 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    34501 cggtcagcat attggcagta gcttcatcgg ttacgatcag gaaacgttct gccggatgtt
    34561 caaaccaaag cagagtagtc gcgccttctt taactacctg tttttctttc gaaggcagtt
    34621 cactaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    34681 gctcgtcgtc tggcgcgatt gtcactttag agaacaccgc atattttttc agttcagtca
    34741 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    34801 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    34861 cttctgtcat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    34921 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    34981 cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    35041 cttaacggcg tattaatcac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    35101 tacgcgatct gtgccttctg atatgctttg cgaggcgact tccaaaaaaa tgaagccctt
    35161 gaattgttgt gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa
    35221 gtcgaaaaac acgttacaat agcggggtac tggattttcg tgagacagga agaagatgga
    35281 cattaacaac aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat
    35341 ttcaatcatg ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat
    35401 ctttattcgt ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg
    35461 taaaccggcc gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga
    35521 acgtggtcct gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc
    35581 tgattcatgg gctggttgcc gctgtaattt tactcatgcc ctggccgctc agttacaccc
    35641 cgttatggat ggtgttactt tcgctggtgg tgtttgattg cgttcgcagc cagcggcgca
    35701 ttaatgctcg ccagggggaa attcgcctgt tgatggacgg gcgtttacgt tggcaagggc
    35761 aggagtggag catcgtcaaa gcgccgtgga tgattaagag tggcatgatg ctgcgtttac
    35821 gttctgatag cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg
    35881 aatggcggga tttacggcgg atattgttgc aacaagagac gcaaagataa agatgacggc
    35941 aaagcatatg tgctaagccg tcagtgacat caggcgtaat gctctgccat ttcgttgagg
    36001 atttgctcgc accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc
    36061 agcgccagac ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca
    36121 tatccttccg ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc
    36181 agcataccga gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac
    36241 agcgcaacaa ttttaccttc gaggttcagg tcgtcgagct gatcccagac ggcttcccag
    36301 tcttcctgga tttcgccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc
    36361 tccattaatt tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata
    36421 atatcgcgga ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga
    36481 cccatattca taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga
    36541 atcgctttag tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc
    36601 aagtgaaaca ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa
    36661 atctggctga aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt
    36721 tgcatcaccg tgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg
    36781 cagaacggct ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc
    36841 gccgattgtt ccagtatctt tatcgcgaaa agtttcgtga agatgatccc agtgcgcatc
    36901 tcgcttcacc gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac
    36961 gtttattaca ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg
    37021 aagtgttgta tgctaccgga ctgcgtgtct ctgaactggt cgggctgaca atgagtgata
    37081 tcagcctgcg tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc
    37141 cgttaggtga agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc
    37201 tgttgaatgg tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc
    37261 gacagacctt ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa
    37321 agctgtcacc gcatgtgttg cgtcacgctt ttgccactca cttattaaat catggtgcgg
    37381 atttacgcgt ggtgcagatg ctactgggcc acagcgatct ctccaccacg caaatttata
    37441 cgcatgtcgc taccgagcgt ctgcgacaac ttcatcaaca gcatcacccg cgggcgtgat
    37501 gtctgaaaag aacaggaaaa tttatgaaga aaggttttat gttatttacc ttgttggcag
    37561 cgttttcagg ctttgttcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg
    37621 gcatcaaaag cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta
    37681 acagcggtgt gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg
    37741 acgttagtgg cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg
    37801 cgcttgaaaa agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt
    37861 ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg
    37921 cgctggggat caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag
    37981 agaaagaaat gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga
    38041 tggcaggtaa aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac
    38101 ttggcgtcca gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg
    38161 ttccgggtta ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga
    38221 ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgag aagtcgatga
    38281 aacggccgac ttgcccgctg aattgcctcc cttgctgcgc cgtttatatg ccagccgggg
    38341 tgtgcgtagc gcgcaggaac tggaacgcag tgttaaaggc atgctgccct ggcagcaact
    38401 gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gcacgcggat
    38461 tattgtggtc ggtgatttcg acgccgacgg tgccaccagc acggctctaa gcgtgctggc
    38521 gatgcgctcg cttggttgca gcaatatcga ctacctggtt ccaaaccgtt tcgaagacgg
    38581 ttacggctta agcccggaag tagtcgatca ggctcatgcc cgtggcgcgc agttaattgt
    38641 cacggtggat aacggtattt cctcccatgc gggggttgaa cacgctcgtt cgttgggcat
    38701 tccggttatt gttaccgatc accatttgcc aggcgacaca ttacccgcag cggaagcgat
    38761 cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt
    38821 ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga
    38881 gcgcggcatc gcaattccta atctggcaga actgctggat ctggtagcgt tggggacagt
    38941 ggcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg
    39001 catccgtgcc ggaaagtgcc gtccggggat taaagcgctg ctggaagtgg ccaatcgcga
    39061 tccacaaaaa ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc
    39121 cgggcgactg gacgatatgt ccgttggcgt ggcgctgttg ttgtgtgaca acattggcga
    39181 agcgcgtgtg ctggcgaatg aactcgatgc actaaaccaa acacgtaaag agatcgaaca
    39241 gggaatgcag gttgaagcgc tcactctgtg cgagaagctg gaacgtagcc gcgacacgct
    39301 gccaggtggg ctggcaatgt atcaccccga atggcatcag ggcgttgttg gcattctggc
    39361 atcgcgtatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag ctggtgatgg
    39421 tacgctgaaa ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg
    39481 attagacaca ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg
    39541 tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac
    39601 ggagtggttg gacccttcgc tattgcaagg cgaagtggtg tcggacggtc cgttaagtcc
    39661 ggccgaaatg accatggaag tggcgcagct gttgcgcgat gctggcccgt gggggcagat
    39721 gttcccggag ccgctgtttg acggtcattt tcgtctgctg caacagcggc tggtgggcga
    39781 acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg acgggattgc
    39841 ttttaatgtc gataccgccc tctggccgga taacggtgtg cgcgaagtgc aactggctta
    39901 caagctcgat atcaacgagt ttcgcggcaa ctgcagcctg caaattatca tcgacaatat
    39961 ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct
    40021 cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc
    40081 ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg
    40141 actatgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg
    40201 tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg
    40261 ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac
    40321 tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc
    40381 tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg
    40441 ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga
    40501 gcatgcttga gcgtatgtat ctgcgctggg ctgaatcgcg tggtttcaaa actgaaatca
    40561 tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg
    40621 attacgctta tggctggctg cgtacagaaa ccggcgttca ccgcctggtg cgtaagagcc
    40681 cgtttgactc cggcggtcgt cgccacacgt cgttcagctc cgcgtttgtt tacccggaag
    40741 ttgatgatga tatcgatatc gaaatcaatc cggcggatct gcgcatcgac gtttatcgcg
    40801 catctggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attactcaca
    40861 tcccaaccgg gatcgtgacc caatgccaga acgaccgttc ccagcacaag aacaaagacc
    40921 aggccatgaa gcagatgaag gcgaagcttt atgaactgga gatgcagaag aaaaatgccg
    40981 agaaacaggc gatggaagac aataaatccg acatcggctg gggtagccag attcgttctt
    41041 atgtccttga tgactcccgc attaaagatc tgcgtaccgg ggtagaaacc cgcaacacgc
    41101 aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat
    41161 gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca
    41221 atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg cgagcagggg attgccttcc
    41281 cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag
    41341 agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgccggccgc atgatgaccc
    41401 gtcgtattat gggtaaagcg tctttcgtta ccctgcagga cgttggcggt cgcattcagt
    41461 tgtacgttgc ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg
    41521 acctcggcga tatcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt
    41581 ctatccactg taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat
    41641 tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta
    41701 acgatgaatc ccgcaacacc tttaaagtgc gctcgcagat cctctctggt attcgccagt
    41761 tcatggtgaa ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atccctggcg
    41821 gtgccgctgc gcgtccgttt atcactcacc ataacgcgct ggatctcgac atgtacctgc
    41881 gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg
    41941 aaatcaaccg taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca
    42001 tgatggaact ctatatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc
    42061 tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtaa
    42121 cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc
    42181 gcccggaaac tgacatggcg gatctggaca acttcgactc tgcgaaagcg attgctgaat
    42241 ctatcggcat ccacgttgag aagagttggg gtctgggccg tattgttacc gagatcttcg
    42301 aagaagtggc agaagctcat ctgatccagc cgaccttcat tactgaatat ccggcagaag
    42361 tttctcctct ggcgcgtcgt aacgacgtta acccggaaat cacagaccgc tttgagttct
    42421 tcattggagg gcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg
    42481 cgcagcgctt cctggatcag gttgccgcga aagatgcagg tgacgacgaa gcgatgttct
    42541 acgacgaaga ttatgtcacc gcactggaac atggattacc gccgacagca ggtctgggaa
    42601 ttggtatcga ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc
    42661 tgttcccggc gatgcgtccg gtaaaataag cattacgtta tgctcacaac cccggcaacc
    42721 gtcggggttt tttatttaag ctgggtaaat gcagataatc gttttctggc ttcgcgattt
    42781 gtcgcctgca tcaccatcca cggactgaac gcccacggcg tagcatcaat accgcgtaaa
    42841 accgctgcta aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca
    42901 ctggtcgtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg
    42961 gtggcgcggt agcgaaagtc aggatagata gattcaggag gcgtaatttc tacgccaagc
    43021 tcataacggc aacggcggat caccgcctct tcgttgcttt ctcccagttg tgggtgccca
    43081 caaaccgagt tagtccacac gccaggccat gcttttttgc taagggcgcg gcgggtaact
    43141 aataattgcc ctttggcatt aaacagccaa ctggaaaacg cgagatgtaa gagggtgtct
    43201 gccgtgtgtg cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa
    43261 atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat
    43321 aatagaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg
    43381 gatgtcgaca ggtaaaaacc atttaaaaaa gcagcatcat atatttgaag cgactttcaa
    43441 tggccattgc aacacaatca gaaatgacag gaatagagag agtaaaaagc aacagcagac
    43501 tgaatatagc gcgcattaaa agattcatat tattctccat gctcattttt cttcagcttg
    43561 cgcatcagaa gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc
    43621 ttcaccaggt cagcatgatg ctggtagcca ttaaagaaga cgttcagcac aacggcgcta
    43681 agggttgcga gcataatgcc gctatgcagc aacggttgca gtacggccgg taacttagaa
    43741 aagaaatcgt gagagagcgt tggcgtcatg ccaacaccga gactgatcgc cacaatatag
    43801 aggttgtaac ggttagtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt
    43861 accatgccga acatcaccag ccccgcgccg cccagcacaa attgcggaat ggaggccacc
    43921 agcaccgcca ttttgggcac catgccgaac aggatcaaaa tgataccgga agcaatgcac
    43981 acccagcggc tatgaacgcg cgtcacgcta accaggccaa cgttttgaga aaatgacgtg
    44041 tgggggaagc tgttaaacgt tccgcctatc attgtcccta caccatcgac acgcagcccg
    44101 cgaataatat cgtgtgaaga gagtttgcga ccgactattt cacccagtgc caggaacatc
    44161 cccatcgact cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga
    44221 tcgaaaatcg gcataccaaa tgacatcggc gtgacaatcg caaaccatga agcatcatgt
    44281 agcccggata aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc
    44341 agtaatacgg cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa
    44401 attaagacgg caaaggagat acctaaataa acgggattac catattgcgg attcccttta
    44461 cctccggcgg cccagtcaat acccacctga atgatgctaa gcccgataga agtaataacc
    44521 acaccggtaa ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat
    44581 gtggtgataa aacccgcggc gatagtggca ccgaatatcc ccagcaggcc gatatccggg
    44641 ttcataccaa tagctatcat tggtgttaca gcggcgaagg tcaccgacat aatcaccggc
    44701 aggcggatcc ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag
    44761 caaaagagat ccgagctgat gagcatcgca atagcttctt ttgataggcc gagtcggtca
    44821 ccaatcataa gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg
    44881 agtatgatca gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc
    44941 ccagaagatg agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc
    45001 cagagtaaaa agcaaccgtt tatgttcatg aaactgtcta aacacatgct cgtggagtgc
    45061 cagtttttgc gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg
    45121 gttaaaccac gaaaatgaga agagaacctg acaatggaat tatctgtaat tttctcatat
    45181 tgatatttca ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctggg
    45241 tagcattctt ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata
    45301 atttgaactc agttgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt
    45361 tattgcatcg cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata
    45421 agtttatcgc tgctgaagct gcggaatgta taggctgcca tgcttgtgaa attgcctgtg
    45481 cggtggcaca taatcaagaa aactggccgc tgagtcacag tgactttcga ccgcgtatcc
    45541 acgttgtagg gaaaggccag gctgcgaatc cggtggcctg ccatcactgc aacaatgccc
    45601 cttgcgttac ggcttgtccg gttaatgctc tgactttcca gtccgatagc gtacaactgg
    45661 acgagcaaaa atgtattggt tgtaaaagat gcgcaatcgc ttgccccttt ggcgtcgttg
    45721 agatggtcga tacgattgca cagaaatgcg acctttgtaa ccagcgcagt tccggcacgc
    45781 aagcctgtat tgacgtctgc ccaacacagg cgttacggct gatggacgat aaagggttgc
    45841 agcagataaa ggtggcccgc cagcgcaaaa cggcagcagg aaaagcgtca tcagacgctc
    45901 agccatctcg cagtgcagcg ttgctccccg ttaactcgcg taaaggcgca gataaaattt
    45961 cagcgagtga acggaaaacc cactttggcg aaatctattg cgggctggat ccacaacaag
    46021 cgacttatga gagtgaccgc tgtgtttatt gtgccgaaaa agctaactgc aactggcatt
    46081 gtccgctgca taacgctatt ccggattaca tccgtctggt acaggaagga aagattattg
    46141 aagcggcaga actttgccac cagaccagtt ccttacccga aatctgcggc agggtatgtc
    46201 cacaggaccg tctttgtgaa ggtgcatgta ctttgaaaga tcactctggc gcagtctcta
    46261 tcggtaatct ggaacgctac atcaccgata ctgcgctggc gatgggctgg cgtcccgatg
    46321 tcagcaaagt tgttccccgt agcgaaaaag tggcggtgat tggcgctgga cctgcaggat
    46381 tagggtgtgc tgatattctg gcgcgcgcgg gagttcaggt tgatgtcttt gatcgccatc
    46441 cagaaattgg cggtatgctg acttttggca ttcctccttt caaactcgat aaaacggtat
    46501 taagccagcg gcgagagata ttcaccgcaa tgggaatcga tttccatctc aactgtgaaa
    46561 ttggccgcga tatctctttc aacgaattaa cggcggaata tgatgctgtt ttcctcggcg
    46621 tggggactta cgggatgatg cgagcagatc tgccgcatga agatgcgcct ggtgtcattc
    46681 aggcgttacc gttcctgacc gcccataccc gccagctcat gggattgccg gagtctgcag
    46741 agtatccgct gacggatgtg gaaggtaagc gagttgtggt attgggcggt ggcgatacga
    46801 caatggattg tttgcggact tccatccgcc ttaatgctgc cagcgtgacc tgtgcgtatc
    46861 gtcgtgatga agtcagtatg ccgggttcgc gcaaagaggt ggtcaatgcc cgcgaggaag
    46921 gtgttgagtt tcagttcaat gttcagccgc aatatatcgc ttgtgatgaa gatggacgct
    46981 taacggcggt gggcctgatt cgtaccgcta tgggtgagcc ggggccggat ggccgccgtc
    47041 gtcctcgtcc ggttgcgggt tcagagtttg aattgcccgc cgatgttctc attatggcct
    47101 ttggtttcca ggcacatacc atgccgtggt tgcagggcag cggaattaaa ctcgataaat
    47161 ggggcctgat tcaaaccggc gacgtcgggt atttacccac ccagacgcat ctgaaaaaag
    47221 tctttgctgg tggtgatgca gttcatggcg cagatctggt tgtcactgca atggccgcag
    47281 gaaggcaggc ggcgcgcgat atgttaactc tgtttgatac gaaggcatcg tgatgaaatc
    47341 gttaattatc gttaatccgg ctgactgtat tggctgccgt acttgtgaag tggcctgtgt
    47401 ggtcgctcac ccttcagaac aggagttgaa tgccgatatc tttttacccc ggctgaaggt
    47461 acaacggctg gatagcatca gtgcgccggt gatgtgccat cagtgtgaaa acgccccttg
    47521 tgttggcgct tgtcccgtgg gggcgctgac gatgggcgag caggtggtgc aggcaaattc
    47581 tgcccgttgt attggctgtc agagttgcgt cagtgcgtgc ccgtttggaa tgatcaccat
    47641 tcagtcattg ccgggggata cccggcaaca aatcgtgaaa tgcgatctct gtgaacagcg
    47701 ggaagagggg ccagcctgcg ttgaatcttg cccgacacag gcgctgcagt tgctgactga
    47761 aagagaactc aggcgagtcc gccagcagcg tattgttgcc agcagtgaga atcctctgtg
    47821 aaagaaatct ccgcgctgaa aggcgcggag aatctattag attgcccaac cacccgcgta
    47881 aaacgcgacc agtgcggcag taatgatcac cgtaccaata ttcagctttt gccattcgcg
    47941 cgcaaagacg cgacccacca ccagtgttac aaagcccagc ataataccgg taacgatatt
    48001 acaagtcaga acgatgaaca cggcacacac caggccagcc attgcgtcaa taaaatcgtt
    48061 gaaatccagc ttcgagacgt tgcttaacat cagcaaaccc acgtacatca gtgcgggagc
    48121 tgtggcataa ccggggatca gaaacgataa cggtgataaa aacagaatca tcaggaacaa
    48181 cacaccaacc acggttgcgg ttaaaccggt tttccccccg gcggcggttc ctgccgctga
    48241 ttcgatataa accgccgcgg gcgctgcgcc caccaggccg gagaatattg aacttactga
    48301 gtcactggtc agggctttgc cgccgttgat gatctggttg tctttatcca acaaattcgc
    48361 ctgaccggcg acggcacgga tggtgccagt ggcgtcgaac actgcggtca tcaccaatgc
    48421 cagtacactc ggaagtacag ttggctggag tgcgcccata atatcgaggc tgaaaatcag
    48481 agacttacca tcttcgccag tcaggcttgg catcgccacc agaccgtggt acttcaccgc
    48541 tgggtcaaag attaagccga tgatcgaaat tgcaataatc accaacaaga tcccgccggg
    48601 tacgcggcac ttttccagac cgaagatgac cgccagcccc agtaagctca tcatcaccgg
    48661 gaaggaggta aaggcaccga gcgccactgg caagccttca atcgggtttt ttataaccat
    48721 tcccacaccg ttagcagcaa tcagcagcag aaacaggccg ataccgatac ctgtaccgtg
    48781 agcgatcccc atcggcaaat tacgtaagat ccaggtacgc acaccggtta cggaaatagc
    48841 ggtgaagatg acgcccatca gaaagactgc gcccagtgcg acaggaacgc taatttgttg
    48901 cccgagtacc agactgaatg cggtaaacgc cgtcaaggaa atagcgcaac caatcgccat
    48961 tggcaaattg gcccataacc ccatcagcaa cgagccgaag cccgcgacca gacaggtggc
    49021 aacaaacaca gctgcgggag gaaaacctgc tttgcccagc attcccggaa cgacgataac
    49081 ggaataaacc atggccagaa aggtcgttaa gccagccagt acttcctgac gaacggtact
    49141 gccacgagcg gtaattttaa aataattatc cagcgcgccc tgtggctttg gtgcgtccgg
    49201 tgtttgtagg atgtctccag acataatgat gtcctcacga ggatgttaaa gttttattat
    49261 tagttgcgtt cgtacaccag acgaccatca acgtaggtgc ggtagatcga acggtcatcg
    49321 cccaacgtca tcatcacgaa caatttgtcg actaaagaaa cagagttgtc atagcgcagc
    49381 tgctgtagcg gagtggcagt gggttccatc accacgaaat cagcctcttt gccaggtaaa
    49441 aagttgccaa tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt ggccaggtaa
    49501 aacgcttcat atgccgagag gcgatagcct tgtaattgca ataccttgta ggcttcgttc
    49561 agcgtttgca gcatgttaaa agttgttccg gcaccgatat ccgttcccat gcccactttg
    49621 actttcttct gccatgcttt tttcaagttg aataagccgc tgccgaggta aaggttggag
    49681 gtcggacaga aagcaatgct ggatttggtt tcgctgagac gatcccactc tttttcttcg
    49741 agatggacgc agtgagcaaa gacgcagttt ttaccggtca ggccgtactg atggtaaaca
    49801 tcaagataac catcatggtc aggataaagc gatttcaccc aggcaatttc atctttgttt
    49861 tcacagagat gggtatgtac ccatgtatcc ggatattctt ctttcaggcg ttgcgccatc
    49921 gccatctgtt caggagatga ggtaggggcg aagcgtggcg taatcgcata caacagacga
    49981 ccatttttgt gccagcgttc aatcagttct ttgctttggt gatagctgct ttcggcagtg
    50041 tcgagcagat aatccggtgc gtttcggtcc atcatcactt taccggcaat catacgcata
    50101 ttgatatgac tggcggcttc aaacagcgca tcaacggatt gtggatgaac agtgccaaac
    50161 accagcgccg tggtggttcc gttacgtaaa agctgcttga tgaagaacgc cgacatctcg
    50221 cgggcgtact ctaaatcctc ataacgacgt tcagtaggga aggtgtgttt attcaaccac
    50281 tccagcaatt gttcaccata ggcccccacc atttcacttt gcggataatg gatatgtgta
    50341 tcgataaagc ccggtactat cagtttgccg cgatagtcgc gcacgcgaat ggtgtcagga
    50401 atttgatgct ttccgtcttc ccattcgcca aaccattcca cttttccctg tttaatgagt
    50461 aataaaccat cctcaataaa ccgcagcgca gaggcaattt cttccgggtt atcgaccgta
    50521 cgggtgacat caataaaact gcctcgtacc gcttttaacg tgtgttctcc tgacatcatt
    50581 gactccttta ctgttaatcc atctcttccg ctgaggtaat accaggcaga acgttttcct
    50641 gtcggtagcc accagggagg ataatattta ataaaatcgc agttaacccg ccagcacaaa
    50701 tagggttttc aactaataca taaatagagg ctggcaatat tttaaaaatt tcgggatcgt
    50761 aggagacgcc aagtcctaaa cctaaagaag tggcgacaat aagtgtttct cggcgcttta
    50821 aaccgttggt gatgatgatg cgaatccctg cgatggcaat catggaaaac atcaacgtca
    50881 ttgcgcctcc cagaactgcc gagggaatgg tcgtaaaaaa gccgccaatc atcggaaata
    50941 agccgaggat aaccagcatt accgcgatgg ttcgcccgac ataacgtgaa gcgacgccag
    51001 tcatctgaat aaccccatta ttttgcgcaa acgtggttaa tggtaatgaa ccgacagcgg
    51061 aggcgataac agaaaccaga ccatctgcca gcacgccgcc tttcagtcgg gactgatact
    51121 cttccccctg aatgggacgg cgggaaacca ttgccgtggc ggtgatatcg ccgacagctt
    51181 ccagcacgct aagcagataa atcgtgccaa ccaccaggaa ctgatggaag ctaaaactaa
    51241 agccgtattt gaacggatgc gggatggtga ttaacggcaa attgcgcata ctgctgaaat
    51301 ccaccatgcc caggcataac gatgcgatat agccgacaca tagcccaatg gcgatcccac
    51361 ccatgcgtag caacggactg cggcagcagt taaagccgat caccacaatc aaaaccaata
    51421 agccaacgcc gagatgttcg taattgccga acgtaccgct gcttttggct gcaaatcctc
    51481 caccaaaatc aataatgccg actttaatca ggcttaagcc gatcatcagc accacaatgc
    51541 cgctgacggt aggcgtaata acccgacgta aatagggcag gataaaagaa gagccgacaa
    51601 ccagaaatgc gccaacgaag gagacgccga ggagcgacga catgattaac tcttcgtgaa
    51661 aaccgtcgct tttcatgctg ctgcccagcg caatcatgac cgtaacaaat gaaaaattga
    51721 ctgactgaat tgaaagtaaa ccagaaccga cgatgccgta gcggtttact tgtaaccagg
    51781 taccaatacc agaggcgatc atcgccatag aaacaagata ggcagttgtt tcagcggaaa
    51841 gctgtaaggc cgcacccacg attaacgcgg gggttaccat cggaacgaaa attgccaaca
    51901 gatgggtaat ggcacctacg agagcctgat gaaagggagg gcgatcctcc agttcaaata
    51961 taaggtcaga acctgcatgg tttatatcag acatcccttc actcctttta aagtaaattt
    52021 gatcatctga attagtaaaa taaattccca cgaatttggg gcgcaggacg ccttgagaac
    52081 ggcttatctc attttcgcag tcactattta gtcatcgttc aaacagagaa acaggccgcc
    52141 gaagcggcca tttattactt atattttttc cagcgcggtg agtattttct ccggtgtgaa
    52201 atgccattcg cgtaaccaga tgccgcaggc atcgtgaatt gcggtagcaa tcgccggagc
    52261 ggcgccattt acaccgattt ccgagatcga tttcgccccg aatgggccga ctttatcgtc
    52321 gctcggcacc agcacagcgc ggaaatcacg cggaatgtca ccaattttcg gtgcgccgta
    52381 actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca taaatgatct cttcgctcat
    52441 actgtggccg atagcacgca gggtggcacc gtagatttgt cccagcgcta actctggatt
    52501 gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc aggcggattt cacccgtacg
    52561 cgtgttgacg gcaacttcag cgaagtttgc gccatacggg aaggcgaaat caggcgtgat
    52621 ataactgcca gttcccacca gtgaaccaaa gccggtgccg gtttcgcctt tatgggctat
    52681 ctctccgaaa ctaacttcgc ctttcttgcc gcgaacgacg cccggcgttg ctagttgaac
    52741 atctgccact ggctcaccca acatttgcgc gccgtggaac aggattttct cccgcagatt
    52801 ttccgctgcc aaacgcgcag cgttacccga gaagcaagta ccggacgagg catatgcgcc
    52861 tttatcaaac aacgcatgat cggtatcacc ggagataaca tgcacgtcct gcggggggca
    52921 gtgcagcact tctgctgcca gtttcgtcac cacagtatcc aggccagtac caatatccgc
    52981 accgccagaa tgaacgataa aggtaccgtc tgattccagt ttgatcatgc agttagcctg
    53041 atcgatatct gggatccctg atttctgcat gataatggcg acgccgcgac cgatgtgcca
    53101 gtcaccgttt tgtggttttg gtgaagacca ttggatcatc tcgcgaccct gacgcaggat
    53161 ctcttccagt gcgcagctgg cggcggaagg aacggaggtc ggcgctttac cttcaccgat
    53221 tgcaccgaga attttcagct cttgcccttc gtgtacccgg ttacgttcga taatttccag
    53281 ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc atggtgatag cgaagttacc
    53341 tttcggtgcg ccataaccct gataagcacc atttgggcaa atgttgctgt agtaggtggt
    53401 gacctggaaa tcgacgttat cgcatggata taacggcagc gacagcgccg gtccgttaca
    53461 cggtacggtg agtgagtggt taccgtaagg gccagtgttg gcgcggaaat ccatcttcac
    53521 tgccgtcagg cgaccatctt ttttcgcgcc cagtttgacg gtgactttcg ctacgtgacg
    53581 agaggtgtta gcaataaact cttcttcacg ggtgtagcgg aaaagtaccg gacgcccggt
    53641 cacgcaagtt gcccaggcgc acacttcttc cagtaggatg tcctgtttag agccaaaacc
    53701 gccgcctaca cgttctttaa tgacatgaac tttatgttgt ttcatgccca cgaggcgcgc
    53761 gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg tggatcacca gacgatcgcc
    53821 gtccatacgg gtaaagcaga tatgtgtttc agtcgggcac tgctgcgcct gcgttgagtt
    53881 ataggttcgc tcaatgatca catcggcatc ggcaaagcct ttgtccatat cgccaatatg
    53941 accatgaata ctggcagcga tatttttgcg tgggcgagaa ccgatcggga agttgatgat
    54001 catatgctcg ccgcgctggg ctgcatggct gttatcatct tctagagtat ctggcgcacc
    54061 agcaacatac accaccggtt catcgtgcac gacaggcgca tcttcagcca ttgcctcgtc
    54121 aatcgacatt accggcttaa gcacttcata ttcaacgtcg atgagcttca gtgcttcgag
    54181 cgcaatgtct tcactttcgg ctactaccgc agcaacgcga tcgccgacgt gacgcatttt
    54241 cttgccgaac attcggcggt caagcggtga cggttccggt gcgctctgac cacccggggt
    54301 atagtaaata tccgggcaat tcaggtgagt aataacgtga acgacgcccg gtaaggcttc
    54361 agctttgctg acatccagat gagtaatcag tgcgtgagcg tgtgggctac gtaacatttt
    54421 aatgacgcag gcgtcagccg ttacgcgatc ttcaacatag cagggtttcg cctgcaccat
    54481 tttcgcggca tcagttttag gataatgctt gccaatgact tcaaggtcgt cacggaaagt
    54541 cggagcgata tcaatggtgg cctgcggatt atttttacgt gcaaccgcca gttcaatgac
    54601 ctgatagtat tgctgccagc cagcatcgcg gctaaacaga cctgagagtg cgtcgtcgat
    54661 ctcttcgcgg gtaggtgcgt cgatgcgatc gagaagatcg gtgataatca gagccgcagc
    54721 tggatcgtta taaccagact gcaccacgcc aacatcaacc atggcctgtt gaaccagact
    54781 taactcattc catttgccca gagattctgc ggtacgaata tctgccttct ctaactgtgc
    54841 ggcgataagc aaggacgcgt taacgatatt accgttaaag ataattgcgt cagaaccggc
    54901 aaacccaaaa ccatcatcac tgttgcgtac agagtgcatt cccatgttaa acaacagctt
    54961 ttgcacgttt ttgcctggat taacggttag ctcctgaggc gcgccattta aagtaaagtg
    55021 gatgatcata cggcttcctc cccagcctgt tggcagtcgg catacagatc ggctaccaga
    55081 actcccgtga tatagcgttt ataggccacg ctgccccgca aatcttcctg cgggaaaatg
    55141 gcgttggcga cagcctgttc aagtgcattg ccttccagat tttgagtttc aacatcatgc
    55201 agacgcagtg ctttactggc gacgccatcc agcgcaattc gcattccgtc gtggtctgtt
    55261 attgcaacgg ctgccgtcac gacggttaaa cctgcctgag aacggctaat tttgcgagtc
    55321 gcacaggtgc gatacggatc tttaatgata atttcggtta acaggcgatc gcatgggcag
    55381 gccaggtagt cctcgattga cagcgtttcg ccgttgccaa aaaccagttc agcatccagt
    55441 gccagcagga cgggaagcag caccgactct tcctgccggg tggcgatttc accaccaatg
    55501 gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga gggcttcacg cagtgctgca
    55561 ggaataaatc gtgcatcacg cagtggctgc aagcgagaca ttgcgccaat tcgtagtgca
    55621 ccgttatccc agtcaatcca gtccagctcc agatcctgca aggaaatggc aatcttttta
    55681 tcggtacggg ttggtgtagc gttgagtttg ctgcccccgg cgaaccagac ggcttcatcc
    55741 tggtagcggc gcttcagttc cagcgcctgt tcgacagagt cgggtctgaa aaattgttca
    55801 atcatcgtct ttccttaatt tcccctcttc tctgaaagaa gaggggagct gttatttatg
    55861 ccagcgcatc catccgacgc cacatactgg cagcggcttt tctggcttgc gcataaatgg
    55921 aatcgcaatc gaagttaaac tgacggtctt catagaccat cacaccattg accatcacgc
    55981 tgtgaacgct gcctgagccc ataccgaaag cgatatgccc ggcgatattg tctgccagca
    56041 gcggcgttgg cgagttgtaa tcgcaaatgg ttaaatcggc tttgtaaccg gcttccagaa
    56101 gcccaaattt cgcgccaaag ttgcggctca tcagttcgtt gccgttagcc agtgctttgg
    56161 caaaactgtc aggccacagc gggccacccg catcgcgatg tttaaagaag gcaaatttca
    56221 tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa cgccagattg cgaatgtcgc
    56281 taaggtgatg gttgtagccg acatggttgt tcatgtttga acgggcgtta tgcaccagga
    56341 acgcatcgcg ctgattgagt agggcgatgt catctttcga caagtacagc ccatgagcga
    56401 ccagcgtttt gctgtcgatg agatcgaatt gcgccagtcg tgccagcagg tctttgccgt
    56461 accaatggtg actgtaggaa acgtcgtaaa ggtcttccgc aacgtgaata tgcaaaccac
    56521 gacctgtgct tttcacggct tcacgcagca tctccagacc ggcatccggt acggtaaacg
    56581 gcgcgtgagc gccgatatgt gcttccacca gatacggctc ggttgctgct ttcttcgcct
    56641 catcaatctg acgggcgaag cggatgtttt cttctacacc ttcctgcaac tctttgatgc
    56701 cgctgttacg gtcagtagtt tcaaaacagg tcatcgcgcg caggccaact tttaaaaatg
    56761 cgtcgcgcaa tgtggagagc gacccgccga tataggctgg agaggcatgg tgatcgataa
    56821 ccgatgtaca tccgctctta atcgcttcca gggaacaaat cagtccgctg taatagagcg
    56881 actcttcatc aagggcgcga tcgagccgcc accagagatt tttcagcgtt gagatgaaat
    56941 ccgggcaggg ggcgatgttt gccataattc cgcgggaaag ccccgagtaa aaatggttat
    57001 gcgagcagac aattcccggc atcacaatcc gaccatgcat ctctttgtag ctggcatcgg
    57061 ggtagcgttg cgtcagggca tcgccgatag cgacaatcac atcattttcg atggcgatat
    57121 caacgccttc ctgcactttc gccgggtgta actgcactgc ggtgacattc ttcagaatca
    57181 acatgattac acctccacgc ggcccagcag ataatgatga tgctggtgga catggctgat
    57241 gatacggcac atatcgttca gctccggtgg tacgttgttg aactgacctt cgctgtcgat
    57301 gtttaacacc cagctttggt tattcagacg tacgcgtacc cggcaatctt ccacaaggaa
    57361 gcctgggttg ctgctgttat caaagtcttg cgacaggctg aagacggtga ttttgtcttt
    57421 gtacggttta ccgttccacg ggcagaattg ggcgcagttg ccgcattcgt tgcagtaggc
    57481 gtcgagatgc agcgtctgga aacggttctg gaagcctggg accgcaatgg atacgttggc
    57541 gcggttcggg cagacatcca cacacttgct gcaaacgtag ttacattcaa ggcagcgagc
    57601 ggcttcctgc gcgacaaacg cgtcacgatc gtcactgttc accagagtga cagagatatc
    57661 gcctttacgt tgatagattt ccgccggatt gacgttattc cagtatttat cgttctggtg
    57721 ggaacggata ttttcccgac tcaggatggc atcggtcgcc cgacgcgcgg ttccgacagc
    57781 agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc ataaagacgt cagtcagacg
    57841 tgtttcgcca ttatggtcga cgtctggcca gccgtttttg tccagcggca cgcccattgc
    57901 attcagggct tcagtatcct gctgttcacc aatggcggtg atcaggctgt ctacatgcag
    57961 cgttactgtt tcgttggttt ctaccggacg gcgacgacct ttctcatcag gttcgccaag
    58021 cgacataacg cgcaaggtta aggtgccatc agcatcgaaa cgttccggat tattcagaaa
    58081 acggaactcc acgccgtcgt gcaacgcttc ttcatactct tcgcgccatg ctggcatctc
    58141 ttgcagtgaa cgacggtaaa cgaccgttgc tttttctacg cctggaacgc gtaacgccgc
    58201 acgagcgcag tccattgcgg tgttacccgc cccgacaacg accacatgtt tgcccagctt
    58261 gagcgcggtg cccttgttgt attcacgcag gaaggggagt gatttccaga cattttggtt
    58321 gtcgcccgcc agtttcacac cgctattttt atcggtaccg gtggcaatca gaacatagtg
    58381 gaagccctga ttttttaact gttcgacggt cagatccggt gagcaaccgt actcaaattt
    58441 cacgccgtga tcggcaacaa aatcgatatc gtgctgaatt aactctgcag gaatacggaa
    58501 ctgaggaatg atatttttca ccacgccgcc cgcattggct tctcgttcaa acagcgtaac
    58561 cggatggccc gctctggcaa ggaagtaacc tgccgccaga cctgccggac ctgcaccaat
    58621 cacggctacc ggatggcgtg aaccggaacc ggctggtttg tgccagcgtt gcttatattc
    58681 atcccaacct ttttccaggg cgactttttt cagttcgcgg atattcagcg cactgtcgta
    58741 atccaggcgg gtacagttgt attggcactg gtgatcgcaa atatgaccgg taatggcggg
    58801 cagggcgttg cgttgataga tgagttccag cgcgtcggca tagcggtgtt cgccaagcaa
    58861 acggatgtat tccggaatat cttgcttgat agcgcaggca gtaacacagg gggcaacgta
    58921 gcaatcggtc agcggcaggt cttcagccac ttcaatacgc tcttctggct tccagtgttt
    58981 ctgggtgtat tccatggtta acgcgtctgc tgccagtctg ttcagtcgtt cgacgtcaac
    59041 gtggttaagc tcccaggcgt cggagccttc cagctcgcgc atgcaggcac ttaagcgcag
    59101 atagccgcca ggtttcagca ggtcggttgc catagtaata gggcgaatac cagtatcaaa
    59161 aatatcgcgg atagtcagct gactggcacc accggaataa gaaatggaca gtttgccgtc
    59221 aaaggcgcga gagagaactg ctgcaacgtt gatggagagc gggaacagcg cacggcctga
    59281 catatacatc tcttcaccgg gcaatgcgcc tttattgttg atggtgccga gagtgttagt
    59341 cagttttacg ccaaagccga gtgatttttc ttttgccagt gccatcaggc gttccagcat
    59401 ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac tcttctttta agccgatgta
    59461 accgaaaccg cagacatcga ggatctcacg aacacgcgcg taccccagta aggtcgggtt
    59521 gagtttcaca aaggtgttga gccctttttc ttccagcatg tagcggcaaa tggcttcgat
    59581 ttcatgcgga ggacagccgt gcatggtgga gagggtaacc ccttgtacca tgctggtggg
    59641 gatgcgagcg ggtaaggctt gcaagctttc gcgtttttcc tgcaatccgt ggcgggctaa
    59701 aaatgcctca tcctggagta gcttattaag tgcatcgcgg tattgagcga atttcggatg
    59761 gtcagatgcg tccatcatat tgtcgatgaa ctgctgcatc ggcggttgct taataccttc
    59821 gaggttgtaa ccgacgctca tattaaagat gaacgattta ccggaatcag aaggctggaa
    59881 catcgcttcg agaaggtgca gggcaaacca ggctttgagg tattcatccc aggctttaag
    59941 cagggtaaat tcggtagacc attcggtgtt aaagcactcg tcttcggcat cgatacaggg
    60001 cttttccagc tccaggcggt caagaatttg gacggttttt agttcgatga agcgtccgcc
    60061 agtcagccag gaggtgacga tgttttgcgc aagttgcgtg tgcgggccag cagcagggcc
    60121 gacgggagtg gcacaggttt caccgaagac gctaacagtt ttacctttta cgggtgagta
    60181 aaactgttgc tcgggaatac caaagattga gcgttgttgt tggtattcat caaatatgcg
    60241 cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc cccataaccc tctccttagc
    60301 ttatacagat atcattgagt gaccggtgtt gagaaatccg gtggtttagg gggatagagc
    60361 aacaatcgca ccactcttgc caggtttggc gggaaatata aaaaaatgtg aggcggctcg
    60421 aaacttaatg gctttttgtg aatgaactca caggtgccaa atgagaatta aaatcactcc
    60481 tgaaaattat tattgccagc ccatttatca ctttttctct tttctcaaaa tcagtgattc
    60541 cttatcatta tgataaagag tgctggaggg attggatatt tgaacgcaat cgtttaacta
    60601 cgttttctaa atttcagtat accttttctt cgctgtaata aagtcgtccg gagtatcaat
    60661 atctaaaatt atttctgcat tttctatttc gacggaataa tgttctccca taaggagtgc
    60721 ctgacgcata ttggtgacat tgggtcgctg gattgcctgc atcaggcatg gttttgacac
    60781 taaaatcgga tggccgggga tgccattatg gagtggcagt attgcgccat catttcgtaa
    60841 cgaccagatt tttctaaaaa tatcgatggt gagggttggc atatctccgt gggtgagaaa
    60901 acaatgttct gtttgcaccg cgggtactgc ggcctttact gatgtcagta aaccctgcgc
    60961 ataatctggg ttgtggataa tagtgatatt gctctggttc gcataacgct cgtgcagttc
    61021 attaccacga tagccggtga ctaaaataat tcggctacaa aactgcaacg catttttgat
    61081 acttgtatca agaattgttc cctgttgcca gggtaacatc attttccatt gccccattct
    61141 tgatgataat cctgcggcgg taattataca gtcgatggct gacatagtga ttccattatt
    61201 tacaggagct aataacgcct gataattgcg ttacttcctg atggattacg ttgagggtaa
    61261 tttatcccag agaggtccat aaagacgcgt gaaaagtata attgacccat cggcattatt
    61321 cattgattta ggcgcgcaga aacgtcccac tgtgatttct attgttggtg ctggggggaa
    61381 aaccagcttg cttttttggc tggcagaact gttccaggcg agtggcaggc gtgtattaat
    61441 cactacgacg acacatatgt ttatgccaac atctcactgg cccgtggttt tctgtcgtga
    61501 tcccgccatg cttcctcatg cgtcttttat atcccccatt tcattttgtt ttcactgctg
    61561 gaaagcgaac cagggaaaag tgcagggatt tacgccagaa gcgattgatg cactggtaca
    61621 acgaccagag tgtgacgtaa ttctcattga ggcagatggc tcgcgtggaa tgccgttaaa
    61681 agcgcctgat gagcacgaac cttgcatacc taaaagcagt tgctgcgtga ttgctgtgat
    61741 gggaggacat attttgggtg cgaaagtgag cacagaaaat gtccatcgct ggtcgcagtt
    61801 tgctgatatt actgggttaa cacctgatgc acccttgcaa ctgagcgatc tcgttgcgct
    61861 ggttcgccac cctcaggggg cgtttaaaaa cgtaccgcaa ggttgtcggc gagtctggtt
    61921 cattaaccgt ttttctcaat gtgagaatgc gattgcgcaa agcgagctcc ttcaaccgct
    61981 gcaacaacac aacgtagagg caatctggct gggcgatata caagaacatc ctgcaatcgc
    62041 gcgcagattt gtgaattagc ggcccgggta ttccgggtta ctgaaaggat atccattgag
    62101 gttggtatga atattttcac agaggctgca aaactcgaag agcaaaattg tccgtttgcg
    62161 atggcgcaaa ttgttgatag ccgaggctca accccccgcc attctgcaca aatgttagtg
    62221 cgcgccgatg gttctatcgt cggtacaatt ggtggcggaa tggttgaacg gaaggtgatt
    62281 gaagagtcgc ttcaggcatt gcaggaacgt aagccgcgat tattccatgg acgtatggct
    62341 cgtaacggtg cggatgctgt cgggtcagat tgtggaggtg caatgtcagt atttatcagc
    62401 gtccatggta tgcgtccacg tctggtgttg attggtgcgg ggcatgtcaa ccgggcgata
    62461 gcccagagtg cggcgctatt aggatttgat atcgccgttg ctgatattta tcgcgaaagc
    62521 ctcaatcctg aactattccc accatcaacc acgcttctcc atgctgagtc gtttggtgcg
    62581 gcagtggaag cactggatat tcgccctgat aattttgtcc tgattgccac gaataatcag
    62641 gatcgtgaag ccctcgacaa actcattgaa caacccattg catggctggg gttgctggca
    62701 agtcgccgca aggttcagct tttcctgcgc caattgcgtg agaaaggcgt ggctgaagaa
    62761 catattgccc gtttacatgc gcccgttggt tacaacatag gtgcggaaac accgcaggag
    62821 atcgccatca gcgtgctggc agaaatatta caggtgaaaa ataacgcgcc gggtgggctg
    62881 atgatgaagc cttctcatcc ttccggacac cagctggtgg tgattcgcgg tgcgggggat
    62941 atcgccagtg gtgtagcgct acgtctgtat catgcgggtt ttaaagtgat catgttggaa
    63001 gtggaaaaac cgacagtgat tcgttgtacc gtggcgtttg cccaggccgt gttcgatggc
    63061 gaaatgacgg tcgaaggcgt caccgcccgc ctggcaacca gctctgcgga agcgatgaaa
    63121 cttaccgaac gcggatttat ccctgtgatg gtagatccca cctgttcatt gcttgatgaa
    63181 ctgaaaccgc tttgcgtggt ggacgctatt ctggcgaaac agaatttggg aacgcgggca
    63241 gatatggcac cagtaacaat cgcgcttggg ccgggcttta ctgcagggaa ggattgtcat
    63301 gcggtaattg aaacaaatcg cgggcactgg ctcggtcagg tgatttactc aggttgtgcg
    63361 caggagaata ccggtgttcc cggcaatatt atggggcata ccacccgacg agtgatccgc
    63421 gctcctgctg caggcattat gcgatccaac gtgaaattag gcgatctggt gaaagagggc
    63481 gatgtcattg cctggattgg tgagcatgaa attaaagcac cgttgacggg gatggtgcgt
    63541 ggcttgttga acgacggact ggccgtggtc ggtggtttta aaattggtga tatcgatcct
    63601 cgtggtgaaa cggctgattt caccagcgtt tctgataaag cccgggcgat tggcggcggc
    63661 gtacttgagg cgttaatgat gttgatgcat cagggcgtta aagcgacaaa agaagtgctg
    63721 gaagtggctt aaatgaaaat ggccggatgg tattacccat ccggccttta aattactgac
    63781 aaatcaccgt tccggttttg ccttcaatcc cttctttcgc tttgctcaat acggtaatca
    63841 gtgcttcacg acctgcgcga gagcgagcaa acgaggcggc agcttccact ttaggcagca
    63901 tagaaccttt cgcaaaatga ccttcttcga taaagcgttc agcatcactt aacgacaggc
    63961 gatcgagcca ctgttcattc tcttttccga agttaatggc cactttttct accgcagtca
    64021 gaatgatcag catatcggca tcgatcattt ctgctaaacg ggcgctggcc cagtctttat
    64081 cgataaccgc gctggcacca cgcagatggt taccttcacg aataacaggg ataccaccac
    64141 cgccaacggt gatcaccacc tgtccggcat ctaccagagc tttaaccgtt tctttttcaa
    64201 tgatatcaac cggttttggc gaagcaacga cacgacgata gccgcgaccc gcatcttctt
    64261 tcagagtgta accctgtttt gtcagtagct ctgcttcttg ctcggtaaag aacgaaccaa
    64321 tcggcttggt cgggttgagg aatgccggat cgttagcatc gacttcgacc tgggtaacca
    64381 gcgttgctac aggtttatta atgccacgag aaagcagttc ttctcttaag gcgttttgta
    64441 gatcgtaacc aatataaccc tggcttaagg caacacagac agacatcggc agcattggcg
    64501 agtgcgcttc agtttttgct gcagcttcaa aggcctggtt aatcatgccc acctgtggcc
    64561 cgttgccatg agtgacgatg acctcatgtc cctgggcaat taaatcaaca attgcctgag
    64621 aagtaatttt taccgctttc atttgcccag ccaggtcatc gcccagcgca ttcccgccca
    64681 gggcgagaac aattttctta ctcatttttc ctcactcatc attcttgatt gtgtatagat
    64741 ccctggcagt ggttagagca cgggagggac aaacggtttg cgtcgcagga aacgacctcg
    64801 cccggctttg cctgtaaagg tgccgtcgca gaaaatcgtt tcaccacggg ataatgttct
    64861 gacaatcgcg ccctgacagg taaacccctc ccagggcgag tagtcggcgt tgtcgtggag
    64921 atggcgatgc tgaatttgtt ggctctgacg tgggtcgata atcaccacgt caccatcgga
    64981 accgggcgct aatatccctt tttgcggcca caggccaaac agcctggcgg gcattgcact
    65041 ggttaactca acaaaacgct ccggtgagat acgtcccgtc atcacgccac tggagaacag
    65101 cagttgcatg cggttctcca caccgggtaa gccatttggg cagcggctga aatcgccttt
    65161 agaaatttgc tggcgctggg ccatcgagaa ggtgcagtga tcggttgcca ccacgtcaat
    65221 cgcaccatcg ctgatgccac accacagttt gtcctgctcg cgtacgttac gcagcggtgg
    65281 gctaagaatg aacttcatgc catcttctgt atcgtaactg cgttcgtcca acaggagata
    65341 ttgtgggcag gtttcaaccc agactggctg gtgattcgca cgggcaagac gcagataatc
    65401 cagacctaag ccgttagaca ggtgcacgat atagagcggg gcgttaccgg caatttgtgc
    65461 caggttaatc atgcgggcga tggcttccgc ttcgcattcc agaggtcgac tcaaggcatg
    65521 ataacgcggc gcggttaacc ctgcggcgat aaactccgcc cgcttgctgg cgatagctgc
    65581 gtcattttcc gggtgtacgg tggtcagcgc gccggactca tgcaagcggc gtaatgcctg
    65641 caacacttca tcatcgttga gtttatattg ataggttaaa tagagtttaa aactgctcag
    65701 tccttcctca accatcatcg gaatttcgtc gaggatagcg tgattaatat gttgaatgac
    65761 accgtgaaag ctgtagtcga tgactgcttt gtgggcggca taaccgcgat aaacttccag
    65821 ttgatggcgt aaccggcagc catttgggcc aaatcccata tggtcaataa tggttgttgt
    65881 accgccacac gcagcagcgc gggtaccggt aaaaaaatca tcacaactgc gcgcgatgcc
    65941 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccaga
    66001 ggcatcaatt tcttcgcaag ggagttgcgg cgaaatatcg gtacccaact ggcgaacaat
    66061 cccgctttca atcagcaaat cctgcttcgc ctgtccatct gcattaacaa caatgccgtt
    66121 ttttatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga gggggataag
    66181 tgaagagtaa ttattcggtt gccagccagc ttaatgggat tgcagcgtac atagcggcac
    66241 aggttaccag gtgagatttc caggtttttt cgtttggcgc gtgcgcttcc ggttctttac
    66301 cagggccaaa accgatgacc ggaatgccgt ggcgacccat gatggatacg ccgttagttg
    66361 agaaggtcca cttatcaacg acaggcgctt taccaaacag cccttcataa gcattcacca
    66421 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
    66481 ggtaaaccag gccagtccag gatgggcggt cgtagttgta catagaaaca accgcgttag
    66541 ctttctgtac tgcaggcagg gcgcggatct cgtccagcgc gccttcccag gtttcgcccc
    66601 aggtcagacg acggtcgatt gaaactgcgc agctgtctgc gacagcgcaa cggcttgggg
    66661 aggtgaagaa aatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
    66721 gacgttggga gagttcttgt aattcgccaa gaattggacc cattttgaaa atggcgttat
    66781 caccacgttc tggtgccgaa ccgtggcagc taacaccctg aacatcaatg cgaatttcca
    66841 tacgaccgcg ttgaccacgg tatacctggc agtcggttgg ttcggtactg accacaaatt
    66901 ccgggcgaat gccggattgt tcaataatgt attgccagca cagaccgtcg cagtcttctt
    66961 cctgcacagt accggtgacc agcagggtat attcatcttc cagaccgaga tctttaatga
    67021 tttttccggc atataccata gacgccatgc cgccttcctg gtcggaagta ccacgaccac
    67081 cgatcagctc gtcggtttcc atgccttcat acggatcgaa gtcccagttt ttgatgttgc
    67141 caatgccgac tgtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
    67201 aaccgagaac attgcccatt gggtcgattt caactttatc gaagccgact ttttccatct
    67261 cttctttaat acgatgtact acgcgtttct cgtcgcagct ttcactggga atagcaacca
    67321 tatcgcgcag gaagcgagtc atgtctgcct ggtaatcttt tgctttttca agaatcagtt
    67381 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
    67441 agcagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
    67501 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcaccacg gcatctttgt
    67561 tcagcgccag tttttccatc aggctttgac gttgcgggtg ataatgaacc gctgcgagaa
    67621 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccatacggat
    67681 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
    67741 cgcagttacg taggatttcc cagcccagtg ggttaggttc gccgcaggcc aggcccgcca
    67801 tgatggtggc catatcaccg ccaacgttga tgatgtcgcc tttgacgccg gagcgataaa
    67861 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctatagacgt
    67921 cgaccagata acccagcaca ccaccggcca ttgcaccgac accggcttgc agcagaacgt
    67981 gtgtcggggt gacgcccatt tcacgcattt gctcgacggc ttcatccgcc agtgttgcgt
    68041 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
    68101 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
    68161 tatccgtgac gatgcactcg gcaccgaggt ttagaatggc gtcaacgcgt tcctgagcag
    68221 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc catgccacac
    68281 cgcgcccgtg gttgccgtcg gtggtagtcg cgaaagtcat tttttcgccg atggcatttt
    68341 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
    68401 cgatggcgta cgcaccgccg agcattttga aggcgttcag accgaatcgt tttgattcgt
    68461 ctttgacgag aattttcttt acaccaaaca ggtttgcgag atcgtccagc gcacaaagcg
    68521 gtgttgggtg ataaccagca attttctggt ggaactggcg tgccagtttt gcctggcttt
    68581 gcgaaaaaag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    68641 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagctga
    68701 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    68761 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    68821 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    68881 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    68941 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    69001 ccagacactg tttttccagt gcttttaagc cttcgtgatc gttcgcacgc agcaattcag
    69061 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    69121 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    69181 ttttagcaac ttcaacgaca tccgggatca ggtcgtagcc ttccggatgg gccagggtga
    69241 catccatacc gaagcgggtc atcagaccta tgatgccttg cggtacagag agcggtttgc
    69301 catagcttgg ggagtaggcc caggtcatgg cgattttttt gcctttcagg ttttccagcg
    69361 aaccaaagtg ttcacgcaac catgccaggt cagccataga ctgagtcggg tggtcaatat
    69421 cgcattgcag gttcactaaa gccggacgct gcggcagcac accctgcttg tagccgtcat
    69481 caagtgcagc gccaacttca cgcatataag cgttgcctgc gcccagatac atatcgtcgc
    69541 gaataccgat agcgtcagcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    69601 cgtgagcgat ttgtgattta ccttcgtcaa gatcttgttg tgcaaggccg agcaggttaa
    69661 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    69721 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aacgcggcgg
    69781 caacgtccag tacttgtttc agttcgtctg gcgtctgttc ccacgttaac aaaaaatctt
    69841 tttcatgcag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    69901 tcataaagtc ccgatagtgg aatgacaggc aaagtggttt atttcactgt gacgtgccaa
    69961 agtgattggt gttttcacag ctatacctgg gactaacaat aagtgtgcca gaattatcca
    70021 cgcggctgcg aaaggctaat ttgacggata atgtgtgggg ttgatcacat attgatgcca
    70081 gtttccggtt gtttttaaaa tacgaaaacg tttggctttt tttatcacgt tatttatttg
    70141 ctgctaagta tcagaaataa gataaagagg caggctatct ctcttcagtt tgataaactt
    70201 gccgctgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tttattagaa
    70261 agtgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    70321 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    70381 tggagcttgc gactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    70441 ccagaatgct tgccagcgtt ttgcagcttg aggttgagat cgttgatgaa aacttgtgtc
    70501 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggccg ccagttgagc ggcaactcac
    70561 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    70621 atcccctttg tgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    70681 cgcctgtcat tttacaggaa cgttgtgttg gggtgataag tttgattgcc gttacccacg
    70741 agcaacaaga acatatcagt gataatttac gcgaattttc cgattacgtt cgccatatat
    70801 ccaccatttt tgtttctaaa cttctggagg atcagggacc aggagataac atcagtaaaa
    70861 tattcgcgac catgatcgat aatatggatc agggcgtgtt agtggttgat gctgataatc
    70921 gggttcagtt tgttaatcag acagctttaa aaacactcgg tgttgtacaa aataatatta
    70981 ttggaaaacc tgttcgtttc agaccattaa catttgagag taattttacc catggtcata
    71041 tgcaacatat tgtttcgtgg gatgataaaa gtgaattaat cattggtcag ttgcataaca
    71101 tccagggccg acaactattt ttaatggcgt ttcaccaatc gcataccagt ttttctgtag
    71161 caaatgcatc tgatgaaccg catattgaac aattggttgg cgagtgccgt gttatgcggc
    71221 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    71281 aaagcggcac gggtaaagaa gtcgtcgccc gtgcaatcca taagttgagc ggaagacgga
    71341 ataaaccctt tattgctatc aactgtgccg cgattccgga acagcttctg gagagcgaac
    71401 tgttcggtta tgttaaaggc gcatttactg gcgcttctgc caacggtaaa acagggttga
    71461 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat tggtgatatg ccattaatgt
    71521 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    71581 gcccaataca agtcgacatc cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    71641 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    71701 ttccaccgct gcgtgaacgt caggaagata ttgaattact ggtgcattac tttttacatc
    71761 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcctgatgtc gtcgaaatat
    71821 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    71881 ttaacgttgt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    71941 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    72001 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    72061 cacgtcataa cagcaagaag gaagttgctg atgaactggg catcggcatt gctacgctct
    72121 atcgcaagat taagaaatat gagttgttaa acacataacc tctgagaatc cggcctgaat
    72181 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    72241 tgataccctg tgcagcgaca aagatttccc gccagtccac gacgaatttc cgtaatggtt
    72301 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gcccggcgta
    72361 caaaacccgc attgcactgc accagatttc gcataagcca gttggacatg agaaagttta
    72421 ccgcctttcg cttcaccttc cagcgtgcgg atctcttttc cttcagccca ggccgcaagg
    72481 aataagcaac tgtctatcgc agtgccgtcg accagcaccg tacaggcacc gcattcgcct
    72541 acgcagcaac cttgtttgac actaagaagc ccttgttctc ggagtagttc cgaaagcggc
    72601 attcctggcg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    72661 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    72721 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    72781 gcggggcgac atcttgcagg actgattcgc tgatggcttc cagcgtttgc aggtttaatg
    72841 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    72901 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    72961 caattgttga aatatccatt gcgtcgcgca tggcatattt aaaatgtgcg ctgcccgcgt
    73021 gttctttcgg ctgtggcgga aaatgaaagg cgacaaggat ttcgtcatgc tcaagagaca
    73081 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgtggggagt
    73141 ggagctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    73201 cgttgcaaat atttccgccg taggtggcga cattacggat ctgcggcccg gcgattgatg
    73261 cagccgcagc acataacgcc gggagattgc gttggattac gggatcttca atgagctgag
    73321 taaatgttgt cgcggagccg attcgcagcg cgccatcttc cgcctgtgtt attccctgaa
    73381 gctctgccag attgtggata tcaacaatat ggcgatagcg gtcattgtga tggtgaagct
    73441 gtatcagtac gtcagtgcca ccggcaagca atttggcctg cggattgtca gccagcaggg
    73501 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    73561 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    73621 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    73681 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    73741 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    73801 ttggggttac ggaccacgcc gcttttcgca tcgatgatca tctcttcaaa tagcgcccag
    73861 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagcgg atttagaata
    73921 tgccccgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag tgcaatatcg
    73981 accgtcagat caacaaaggt acagccgaac gccggtgggt tagtggtggt tttgatggag
    74041 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    74101 tttaacgaca ttaacggttc ttccggtcgt tcaatcagca cgatatggcc ttttatcagg
    74161 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    74221 aataacagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    74281 gcgccgggat cgaacggcgt aacatcggta tcttgtgttg aaataacgcg aacgtcgctg
    74341 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    74401 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcaa caggcgcgcg
    74461 cctgctattt ctacgccgac aggccaggtg ttagaggtgt agctaaaaca ggcgacgcca
    74521 acgccacgac gtaaattgcc ttgctggttc tggcactctg cacggcgttt ttcccattca
    74581 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgttttccc
    74641 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    74701 atacctaacg ctgtcgcggc atcatcaagc atagactcaa cggcaaatac gacttgtggc
    74761 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    74821 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tccccccagc agaagcaatg
    74881 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    74941 aatgttccgt cgcggttcac gcccatttgc ccgtcaatag taaaagcgtg gcgggtacgg
    75001 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagtttg
    75061 ctggtcagga atgccgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    75121 ccgataaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    75181 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    75241 tccatccatg ccagcgatgt cacgctttcc atatgacaat gttgaataac gggagtctga
    75301 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    75361 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    75421 gccgcttctg gcgaggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    75481 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc gccatgatga
    75541 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    75601 caggcatgcc ctgccgtggc gaatgggatt tctggcacat cttcccaggt aaaaatcgcc
    75661 aggacgcccg gcaaactcct ggcttgttca tcattaatac ttacagcata accatgtgcg
    75721 atagggctac gtacatattt cgcgtaacac atgcccgcca taacataatc gtcagtatat
    75781 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    75841 gttgcttccc gcgcttccat agaaatcccc tcggtttgaa catttggttc ttatggcaaa
    75901 gatttagtct tagcaggcaa gaattacgcc agaaacgact atatgcgtga ggggaggaag
    75961 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaatagggac
    76021 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattagg attattacgc
    76081 atcaaggtgg gaaatagggt aaaagtcagg aacttcgata ccactacagc aaatattttg
    76141 ctgatgatac ttccctttgc caaatgaaat taacctcttt ctctattaca tcgcttttcc
    76201 agaagcaaga gattgggtat ccagaagccg gctgctatga tatggcgtct tgttttttaa
    76261 gcgaggaaag attttgagtg cgggacgcct gaataaaaaa tctctgggta tcgtgatgtt
    76321 gttatcggtt gggctgcttt tggcgggctg ttcgggtagc aaatcatccg atacaggaac
    76381 gtattccggc tccgtttaca ccgtgaaacg gggggatacg ctatatcgta tttcgcgcac
    76441 cacgggaacc agcgtaaaag aactggcgcg actgaacggc atttcccccc cttacaccat
    76501 tgaagtaggt cagaaactaa aactgggtgg ggcgaaaagt agcagtagta cacgtaaatc
    76561 aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg tcatcagcgg taccgaaatc
    76621 ttcctggccg ccagtagggc aacgttgttg gttatggcca acgacaggga aagttatcat
    76681 gccgtattcg acagcagatg gcggcaataa agggattgat atctcagctc cacggggtac
    76741 acctatttac gccgcgggtg caggaaaggt ggtgtatgtg ggcaaccagc tgcgtggcta
    76801 cggtaatctc atcatgatta aacacagtga agattacatt acggcttacg ctcataatga
    76861 cacgatgctg gtaaataatg ggcaaagcgt gaaagctggg caaaaaatcg ccactatggg
    76921 gagcactgat gcggcatctg ttcgcctgca tttccagatt cgttaccgtg caacggcaat
    76981 tgatccgcta cgttacttgc cgcctcaggg cagcaagcca aaatgctgat ggcgaattaa
    77041 tcagcagtca gcagcgtggt acttgctaag gagagcgtaa ggtttataat gccttacgca
    77101 tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc ccaagctcta tacgagggtt
    77161 cgattccctt cgcccgctcc aatttatctt cacatcatct acctgccatt tttgttgtat
    77221 ctgattttct atattatctt aattcaagcg cttcagactt ttcgcaatcg ccgtaatact
    77281 tccccatttt ttgtaaataa aaagcgt
//
LOCUS       DADTFG010000004        67090 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.004, whole genome shotgun sequence.
ACCESSION   DADTFG010000004 DADTFG010000000
VERSION     DADTFG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.004"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            64..1461
                     /gene="ulaA"
                     /locus_tag="J1138_000286"
     CDS             64..1461
                     /gene="ulaA"
                     /locus_tag="J1138_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBA8452134.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1477..1782
                     /gene="ulaB"
                     /locus_tag="J1138_000287"
     CDS             1477..1782
                     /gene="ulaB"
                     /locus_tag="J1138_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBA8452135.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1792..2256
                     /gene="ulaC"
                     /locus_tag="J1138_000288"
     CDS             1792..2256
                     /gene="ulaC"
                     /locus_tag="J1138_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBA8452136.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEANFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2270..2920
                     /gene="ulaD"
                     /locus_tag="J1138_000289"
     CDS             2270..2920
                     /gene="ulaD"
                     /locus_tag="J1138_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBA8452137.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2930..3784
                     /locus_tag="J1138_000290"
     CDS             2930..3784
                     /locus_tag="J1138_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBA8452138.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3784..4470
                     /locus_tag="J1138_000291"
     CDS             3784..4470
                     /locus_tag="J1138_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBA8452139.1"
                     /translation="MQKLKQQVFEANMDLPHYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGNVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4599..4874)
                     /locus_tag="J1138_000292"
     CDS             complement(4599..4874)
                     /locus_tag="J1138_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA8452140.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5202..5597
                     /gene="rpsF"
                     /locus_tag="J1138_000293"
     CDS             5202..5597
                     /gene="rpsF"
                     /locus_tag="J1138_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBA8452141.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5604..5918
                     /gene="priB"
                     /locus_tag="J1138_000294"
     CDS             5604..5918
                     /gene="priB"
                     /locus_tag="J1138_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBA8452142.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5923..6150
                     /gene="rpsR"
                     /locus_tag="J1138_000295"
     CDS             5923..6150
                     /gene="rpsR"
                     /locus_tag="J1138_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBA8452143.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6192..6641
                     /gene="rplI"
                     /locus_tag="J1138_000296"
     CDS             6192..6641
                     /gene="rplI"
                     /locus_tag="J1138_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBA8452144.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            7043..7534
                     /locus_tag="J1138_000297"
     CDS             7043..7534
                     /locus_tag="J1138_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8452145.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     SSGKNEEGIQTDPFQTRYEKEGLLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            7537..8805
                     /locus_tag="J1138_000298"
     CDS             7537..8805
                     /locus_tag="J1138_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBA8452146.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKTSVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMVVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDTSTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGYILIPGK
                     GWRLSEAYDLNPVARSDGLKLNITENDNALDLELAREVAEYFRLGLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRGAI"
     gene            complement(8948..10225)
                     /locus_tag="J1138_000299"
     CDS             complement(8948..10225)
                     /locus_tag="J1138_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452147.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATS"
     gene            complement(10318..12390)
                     /locus_tag="J1138_000300"
     CDS             complement(10318..12390)
                     /locus_tag="J1138_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBA8452148.1"
                     /translation="MSQLDEAILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRQVFMDFNRNPEPVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQQMNPLSISLYKMHGYDLITQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRIQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            complement(12387..13928)
                     /locus_tag="J1138_000301"
     CDS             complement(12387..13928)
                     /locus_tag="J1138_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBA8452149.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVALVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPFMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(13938..14714)
                     /locus_tag="J1138_000302"
     CDS             complement(13938..14714)
                     /locus_tag="J1138_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBA8452150.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPHAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(14724..15566)
                     /locus_tag="J1138_000303"
     CDS             complement(14724..15566)
                     /locus_tag="J1138_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA8452151.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQIARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(15576..16367)
                     /locus_tag="J1138_000304"
     CDS             complement(15576..16367)
                     /locus_tag="J1138_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBA8452152.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            16580..17227
                     /locus_tag="J1138_000305"
     CDS             16580..17227
                     /locus_tag="J1138_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA8452153.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(17211..17849)
                     /gene="ytfB"
                     /locus_tag="J1138_000306"
     CDS             complement(17211..17849)
                     /gene="ytfB"
                     /locus_tag="J1138_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBA8452154.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            18067..18687
                     /locus_tag="J1138_000307"
     CDS             18067..18687
                     /locus_tag="J1138_000307"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="FKBP-type; rotamase; catalyzes the cis-trans
                     isomerization of proline imidic peptide bonds in
                     oligopeptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA8452155.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDAVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            18996..20408
                     /gene="cycA"
                     /locus_tag="J1138_000308"
                     /gene_synonym="dagA"
     CDS             18996..20408
                     /gene="cycA"
                     /locus_tag="J1138_000308"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBA8452156.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     AGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(20453..21115)
                     /gene="ytfE"
                     /locus_tag="J1138_000309"
     CDS             complement(20453..21115)
                     /gene="ytfE"
                     /locus_tag="J1138_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBA8452157.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(21223..22188)
                     /locus_tag="J1138_000310"
     CDS             complement(21223..22188)
                     /locus_tag="J1138_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA8452158.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWGGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(22297..23157)
                     /gene="qorB"
                     /locus_tag="J1138_000311"
     CDS             complement(22297..23157)
                     /gene="qorB"
                     /locus_tag="J1138_000311"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBA8452159.1"
                     /translation="MIAITGATGQLGHYVINSLLKTVPASQIVAIARNPAKAQALSAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPPGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            23246..23626
                     /locus_tag="J1138_000312"
     CDS             23246..23626
                     /locus_tag="J1138_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBA8452160.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(23755..25698)
                     /gene="cpdB"
                     /locus_tag="J1138_000313"
     CDS             complement(23755..25698)
                     /gene="cpdB"
                     /locus_tag="J1138_000313"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBA8452161.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSANPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            25888..26628
                     /gene="cysQ"
                     /locus_tag="J1138_000314"
     CDS             25888..26628
                     /gene="cysQ"
                     /locus_tag="J1138_000314"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBA8452162.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(26618..27175)
                     /locus_tag="J1138_000315"
     CDS             complement(26618..27175)
                     /locus_tag="J1138_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBA8452163.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLSHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            27500..27706
                     /locus_tag="J1138_000316"
     CDS             27500..27706
                     /locus_tag="J1138_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBA8452164.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(27784..29127)
                     /locus_tag="J1138_000317"
     CDS             complement(27784..29127)
                     /locus_tag="J1138_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBA8452165.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(29450..30088)
                     /gene="msrA"
                     /locus_tag="J1138_000318"
     CDS             complement(29450..30088)
                     /gene="msrA"
                     /locus_tag="J1138_000318"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBA8452166.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            30294..32027
                     /gene="tamA"
                     /locus_tag="J1138_000319"
     CDS             30294..32027
                     /gene="tamA"
                     /locus_tag="J1138_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBA8452167.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32024..35803
                     /gene="tamB"
                     /locus_tag="J1138_000320"
     CDS             32024..35803
                     /gene="tamB"
                     /locus_tag="J1138_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBA8452168.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            35806..36147
                     /locus_tag="J1138_000321"
     CDS             35806..36147
                     /locus_tag="J1138_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBA8452169.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            36297..37769
                     /locus_tag="J1138_000322"
     CDS             36297..37769
                     /locus_tag="J1138_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBA8452170.1"
                     /translation="MKKSLLAVMLTGLFALVSLPALGNVNLEQLKQKAESGEAKAQLE
                     LGYRYFQGNETTKDLTQAMDWFRRAAEQGYTPAEYVLGLRYMNGEGVPQDYAQAVIWY
                     KKAALKGLPQAQQNLGVMYHEGNGVKVDKAESVKWFRLAAEQGRDSGQQSMGDAYFEG
                     DGVTRDYVMAREWYSKAAEQGNVWSCNQLGYMYSRGLGVERNDAISAQWYRKSATSGD
                     ELGQLHLADMYYFGIGVTQDYTQSRVLFSQSAEQGNSIAQFRLGYILEQGLAGAKEPL
                     KALEWYRKSAEQGNSDGQYYLAHLYDKGAEGVAKNLEQAISWYTKSAEQGDATAQANL
                     GAIYFRLGSEEEHKKAVEWFRKAAAKGEKAAQFNLGNALLQGKGVKKDEQQAAIWMRK
                     AAEQGLSAAQVQLGEIYYYGLGVERDYVQAWAWFDTASTNDMNLFGTENRNITEKKLT
                     AKQLQQAELLSQQYIEKYAPEAWARMQKLKAQSAVKTGNK"
     gene            complement(38183..38713)
                     /gene="ppa"
                     /locus_tag="J1138_000323"
     CDS             complement(38183..38713)
                     /gene="ppa"
                     /locus_tag="J1138_000323"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBA8452171.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            39023..39979
                     /gene="ytfQ"
                     /locus_tag="J1138_000324"
     CDS             39023..39979
                     /gene="ytfQ"
                     /locus_tag="J1138_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBA8452172.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEILVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            40119..41621
                     /locus_tag="J1138_000325"
     CDS             40119..41621
                     /locus_tag="J1138_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA8452173.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            41635..42657
                     /locus_tag="J1138_000326"
     CDS             41635..42657
                     /locus_tag="J1138_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8452174.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDIFNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGG
                     RNRDKT"
     gene            42644..43639
                     /gene="yjfF"
                     /locus_tag="J1138_000327"
     CDS             42644..43639
                     /gene="yjfF"
                     /locus_tag="J1138_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBA8452175.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGILLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(43672..44670)
                     /gene="fbp"
                     /locus_tag="J1138_000328"
     CDS             complement(43672..44670)
                     /gene="fbp"
                     /locus_tag="J1138_000328"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBA8452176.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            44846..46219
                     /gene="mpl"
                     /locus_tag="J1138_000329"
     CDS             44846..46219
                     /gene="mpl"
                     /locus_tag="J1138_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBA8452177.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(46377..46928)
                     /locus_tag="J1138_000330"
     CDS             complement(46377..46928)
                     /locus_tag="J1138_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBA8452178.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            47022..48374
                     /gene="pmbA"
                     /locus_tag="J1138_000331"
     CDS             47022..48374
                     /gene="pmbA"
                     /locus_tag="J1138_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBA8452179.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            48429..48815
                     /gene="cybC"
                     /locus_tag="J1138_000332"
     CDS             48429..48815
                     /gene="cybC"
                     /locus_tag="J1138_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBA8452180.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AVQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(48860..49324)
                     /gene="nrdG"
                     /locus_tag="J1138_000333"
     CDS             complement(48860..49324)
                     /gene="nrdG"
                     /locus_tag="J1138_000333"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBA8452181.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(49482..51620)
                     /gene="nrdD"
                     /locus_tag="J1138_000334"
     CDS             complement(49482..51620)
                     /gene="nrdD"
                     /locus_tag="J1138_000334"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBA8452182.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(52014..53669)
                     /gene="treC"
                     /locus_tag="J1138_000335"
     CDS             complement(52014..53669)
                     /gene="treC"
                     /locus_tag="J1138_000335"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBA8452183.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLTILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCTMTLRPFEAVWWLQK"
     gene            complement(53719..55140)
                     /gene="treB"
                     /locus_tag="J1138_000336"
     CDS             complement(53719..55140)
                     /gene="treB"
                     /locus_tag="J1138_000336"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBA8452184.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPVNAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(55259..56206)
                     /gene="treR"
                     /locus_tag="J1138_000337"
     CDS             complement(55259..56206)
                     /gene="treR"
                     /locus_tag="J1138_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBA8452185.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKTHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            56391..56444
                     /gene="mgtL"
                     /locus_tag="J1138_000338"
     CDS             56391..56444
                     /gene="mgtL"
                     /locus_tag="J1138_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBA8452186.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            56585..59281
                     /gene="mgtA"
                     /locus_tag="J1138_000339"
     CDS             56585..59281
                     /gene="mgtA"
                     /locus_tag="J1138_000339"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBA8452187.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNSLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVELDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(59487..59873)
                     /gene="ridA"
                     /locus_tag="J1138_000340"
     CDS             complement(59487..59873)
                     /gene="ridA"
                     /locus_tag="J1138_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBA8452188.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(59946..60407)
                     /gene="pyrI"
                     /locus_tag="J1138_000341"
     CDS             complement(59946..60407)
                     /gene="pyrI"
                     /locus_tag="J1138_000341"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBA8452189.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(60420..61355)
                     /gene="pyrB"
                     /locus_tag="J1138_000342"
     CDS             complement(60420..61355)
                     /gene="pyrB"
                     /locus_tag="J1138_000342"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBA8452190.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(61359..61493)
                     /gene="pyrL"
                     /locus_tag="J1138_000343"
     CDS             complement(61359..61493)
                     /gene="pyrL"
                     /locus_tag="J1138_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBA8452191.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(61583..62071)
                     /locus_tag="J1138_000344"
     CDS             complement(61583..62071)
                     /locus_tag="J1138_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBA8452192.1"
                     /translation="MKEYDDYSAKEKKQLAVCQRLITEKSYLSQEEIRRDLQNHGFDS
                     ISQSTVSRLLKLLGVIKIRNTKGQKIYSVNPQLLPTPDAGRSVAEMVLSVEHNGEFIL
                     IHTVAGYGRAVARILDFHALPEILGVIAGSNIVWVAPRVVKRTALVYKQINYLLKLNI
                     YS"
     gene            complement(62187..63590)
                     /locus_tag="J1138_000345"
     CDS             complement(62187..63590)
                     /locus_tag="J1138_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBA8452193.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAAAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLAVLTMPI
                     MAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
     gene            complement(63647..64651)
                     /gene="argF"
                     /locus_tag="J1138_000346"
     CDS             complement(63647..64651)
                     /gene="argF"
                     /locus_tag="J1138_000346"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBA8452194.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDEALVTQCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            complement(64662..65606)
                     /gene="arcC"
                     /locus_tag="J1138_000347"
     CDS             complement(64662..65606)
                     /gene="arcC"
                     /locus_tag="J1138_000347"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA8452195.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLPQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKGWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPITTLDPFL"
     gene            complement(65617..66837)
                     /gene="arcA"
                     /locus_tag="J1138_000348"
     CDS             complement(65617..66837)
                     /gene="arcA"
                     /locus_tag="J1138_000348"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBA8452196.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGVEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDVNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
BASE COUNT        16299 a        16710 c        17649 g        16432 t
ORIGIN      
        1 gagtgagagc acaacattcc gggtgtgcga aatacccggt tacctcttct tcaggagatc
       61 gttatggaga tcctctacaa catctttacc gtgtttttta accaggtcat gaccaatgcc
      121 ccgttgttgc tgggtattgt gacctgtctg ggttacatcc tactgcgcaa aagtgtcagc
      181 gttattatta aaggtacgat taaaaccata attggtttca tgttgttgca ggcagggtcc
      241 ggcatcctga ccagcacctt caaaccggtg gtggcgaaaa tgtccgaagt ctacggcatt
      301 aacggcgcaa tttccgatac ttacgcgtcg atgatggcaa ccattgaacg catgggcgat
      361 gcctatagct gggtgggtta cgcggtgttg ttagcgctgg cgctgaacat ctgttacgtg
      421 ctgctgcgcc gaattaccgg tattcgcacc atcatgctga ctggtcacat catgttccag
      481 caggcggggc tgattgccgt tacgctgttt atcttcggct actccatgtg gacgaccatt
      541 atctgcaccg cgattctggt ttcgctctac tggggcatta cctccaacat gatgtacaag
      601 ccgacacagg aagtcaccga cggctgtggt ttctccattg gtcaccagca acagtttgca
      661 tcatggattg cctataaagt tgcgccgttc ctcggcaaaa aagaggaaag cgttgaagac
      721 ctcaaactgc caggctggct gaatattttc cacgacaaca tcgtctccac agcgattgtc
      781 atgactatct tctttggtgc cattctgctc tcctttggta tcgacaccgt gcaggcgatg
      841 gcaggcaaag tgaactggac ggtgtacatc ctgcaaaccg gtttctcctt tgcggtggcg
      901 attttcatca tcactcaggg cgtgcgcatg tttgtggcgg agctctctga agcattcaac
      961 ggtatctccc agcgcctgat cccgggtgcg gttctggcga ttgactgtgc ggctatttat
     1021 agcttcgcgc cgaacgccgt ggtctggggt tttatgtggg gcaccatcgg gcaactgatt
     1081 gcggttggca tcctggtcgc ctgtggctct tcaatcctga ttattccagg ctttatcccg
     1141 atgttcttct ccaacgccac tatcggcgtg ttcgctaacc acttcggcgg ctggcgcgcg
     1201 gcgctgaaga tttgcctggt gatggggatg atcgaaatct ttggttgcgt ctgggcggtg
     1261 aaactcaccg gtatgagcgc ctggatgggc atggcggact ggtcgattct ggcaccgccg
     1321 atgatgcagg gcttcttctc catcggtatc gcctttatgg ccgtcatcat tgtcattgca
     1381 ctggcttata tgttcttcgc tggccgcgca ctgcgtgcag aagaagatgc agaaaaacaa
     1441 ctggcagaac aatctgctta ataaggagtt ttgattatga ccgtacgtat tctggctgtg
     1501 tgtggtaacg gacaaggcag ttccatgatc atgaaaatga aagtggacca gtttttaacc
     1561 cagtcgaaca tcgaccacac ggtaaacagc tgcgcggttg gcgaatacaa aagcgagttg
     1621 agcggtgcgg atatcatcat cgcttctacc catattgcgg gcgaaatcac cgtgaccggc
     1681 aacaaatatg tggttggcgt gcgcaacatg ctctctcctg ctgattttgg cccgaaactg
     1741 ctggaagtga tcaaagagca tttcccgcag gacgtgaagt aaggacgcgc catgaaatta
     1801 cgtgattcgc tggctgaaaa taaatctatc cgcctgcaag cggaagcaga gacatggcag
     1861 gacgcagtga aaatcggcgt tgatctgctg gtagcggcag atgtggtaga gccgcgttac
     1921 taccaggcga ttctggatgc ggttgaacag cacggcccgt actttgtgct cgcacccggc
     1981 ctggcaatgc cgcacggacg cccggaagag ggggttaaga aaactggttt tgcgctggtg
     2041 acgctgaaaa agccgctgga gttcaaccac gaagataacg acccggtgga tatcctcatc
     2101 accatggcgg cggtcgatgc caacacgcat caggaagtgg gcatcatgca gatcgtcaac
     2161 ctgtttgaag atgaagccaa tttcgaccgt ttacgcgcct gccgtaccga gcaggaagta
     2221 ctggatttaa ttgaccgcac caacgcggca gcttaagaag gaattgaaga tgtcattacc
     2281 gatgttgcaa gtcgcgctgg acaaccagac tatggatagc gcctacgaaa ccactcgcct
     2341 gattgccgaa gaagtcgaca ttatcgaagt gggcaccatt ctttgcgtag gcgaaggcgt
     2401 gcgtgcggtt cgtgacttga aagcgctgta cccgcacaaa atcgtactgg ctgacgccaa
     2461 aattgccgat gcaggcaaaa tcctttctcg catgtgcttc gaagccaacg ctgactgggt
     2521 gaccgtaatt tgctgtgcgg atatcaacac cgccaaaggc gcgctggacg tggcaaaaga
     2581 gtttaatggc gacgtgcaga tcgaattgac cggctactgg acctgggaac aggcgcagca
     2641 gtggcgcgac gcaggcattg ggcaggtggt ttatcaccgc agccgtgacg cgcaggccgc
     2701 aggcgtggca tggggcgaag cggacattac cgcgatcaaa cgtctttccg atatgggctt
     2761 caaagtcacc gtcaccggtg gtctggcgct ggaagatctg ccgttgttta aaggcattcc
     2821 gattcatgtg tttatcgctg gtcgcagcat tcgtgatgcc gcttctccgg tggaggccgc
     2881 acgtcagttc aaacgttcca tcgctgaact gtggggctaa ggagcggata tgttgtccaa
     2941 acaaatcccg cttggcatct acgaaaaagc gctccccgct ggggagtgct ggctggaacg
     3001 actgcaactg gcaaaaacgt taggcttcga ttttgtcgag atgtcagtag atgaaacgga
     3061 cgatcgcctt tcgcgcctcg actggagccg cgagcagcgt ctggcactgg tgaatgcgat
     3121 tgttgaaacc ggcgtgcgcg taccgtccat gtgcctttct gctcatcgtc gtttcccgct
     3181 gggaagcgaa gatgacgcgg tgcgggcgca ggggctggag attatgcgta aagctatcca
     3241 gttcgcccag gacgtcggca ttcgcgtgat ccaactggcg ggctatgacg tttactatca
     3301 ggaagccaat aacgaaacgc gtcgtcgttt ccgtgacggc ctgaaagaga gcgttgagat
     3361 ggcaagccgt gcgcaggtca cgctggcgat ggagatcatg gattacccat tgatgaactc
     3421 catcagcaag gcgctgggct acgcacacta tctcaacaat ccgtggttcc agctctaccc
     3481 ggatatcggc aacctgtcgg cgtgggataa tgacgtgcag atggagttgc aggctggaat
     3541 cgggcacatc gtcgcggtgc atgtgaaaga caccaaacca ggcgtcttca aaaacgtgcc
     3601 gtttggcgaa ggcgtagtgg atttcgaacg ttgcttcgaa acgctcaaac agagtggcta
     3661 ttgcgggccg tacctgattg agatgtggag cgaaacggcg gaagacccgg cggcagaagt
     3721 agcgaaagcg cgtgattggg tgaaagcgcg catggccaaa gcaggcatgg tggaggcggc
     3781 ataatgcaaa agctgaaaca gcaggtgttt gaagccaaca tggatttacc gcattacggg
     3841 ttggtgacct ttacctgggg caacgtcagc gctattgacc gtgaacgcgg gctggtggtg
     3901 atcaagccca gcggtgtcgc ctacgaaacc atgaaagcgg acgatatggt agtggtcgat
     3961 atgagcggca acgtggtgga aggggagtat cgtccgtctt ccgacactgc gacgcatctc
     4021 gaactctacc gtcgttatcc gtcgcttggc ggcattgtcc atacccactc cactcatgcc
     4081 accgcatggg cgcaggcggg gctggcgatc ccggcgttag gcaccacgca cgccgattac
     4141 ttctttggcg acatcccgtg cacgcgcgga ttaagcgaag aagaggtgca gggtgagtac
     4201 gaactgaata ccggcaaagt gattatcgaa acgctgggtg acgccgagcc gctgcataca
     4261 ccggggattg tggtgtacca gcatggacca tttgcctggg ggaaagatgc ccacgatgcg
     4321 gtgcataacg cggtggtgat ggaagaagtg gcgaaaatgg cgtggattgc gcgcagtatt
     4381 aacccacaac tgaatcacat cgacagcttc ctgatgaata aacacttcat gcgtaagcac
     4441 ggtcccaacg cctattacgg gcagaagtaa aacacgcgct gcggaaattt ccttcttcgg
     4501 gagataactg gtctaattcc gcagccgttt ttcaaaaaaa gccccctgcg aagggggcaa
     4561 agcaaactat ggcaatgttt cgttggttat acctggtgct agcgataaat atccgcgctg
     4621 gcgtgcatat tgccgttact ccccggttcc cgcatcagaa tcacatggta gtacgttgcg
     4681 ccttgcgcat ctgtctcttc atttaatgcc tgacgtgctt cactttcagt ggcgaagtta
     4741 tggttgatat aaatcacgcc taagctttgc acatcatcca tatttctggc ctggtggtta
     4801 ttaatttcaa tggctgccca tgtatttgca cttagcaaaa gcacagccag aagggctaaa
     4861 acacgactga acatagatac ctcctcgacg gctgactttg tgtgctctcc tcttgtggtg
     4921 atcttctgat ttaattttaa tcaatgataa agaagttgat ggtgaccatt tctgatgcag
     4981 ttgttcaaaa aaacaccatg atgaagtgtg atgaactcca aatcagcgat ttagaggtta
     5041 tttgcgaaag gggagattta tttcggctct gcccttgagt ttagcggggt atgtcagtac
     5101 tataacgcgt catttttcag ccgaccttta acacgttcct tgcctccccg ggattcggct
     5161 gacccagaca ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat tacgaaatcg
     5221 tttttatggt ccatcctgac cagagcgaac aggttccggg catgatcgag cgctacactg
     5281 ctgccatcac tggtgcagaa ggcaagatcc accgtctgga agactggggc cgccgtcagc
     5341 tggcttaccc gatcaacaaa ctgcacaaag cacactacgt tctgatgaac gttgaagctc
     5401 cgcaggaagt gatcgatgag ctggaaacta ccttccgctt caacgatgcc gttatccgca
     5461 gcatggttat gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt aaagcgaaag
     5521 acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat gctgaagctg
     5581 gggattctga agagtaattt ctgatgacca accgtctggt gttgtccggc accgtgtgca
     5641 ggactcccct tcgaaaggtc agtccatcag gaattcctca ctgccagttc gtgcttgagc
     5701 atcgttctgt gcaggaggaa gccggctttc accggcaggc gtggtgccaa atgcccgtta
     5761 ttgttagcgg acacgaaaac caggccatta ctcacagtat aacggtcggc agtcgcataa
     5821 ccgttcaggg gttcatttca tgccataagg caaagaacgg actgagcaaa atggttttgc
     5881 atgccgagca gattgaattg atagattctg gagactagcc atatggcacg ttatttccgt
     5941 cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag agatcgacta taaagatatc
     6001 gctacgctga aaaactacat caccgaaagc ggtaagattg tcccaagccg tatcaccggt
     6061 acccgtgcaa aataccagcg tcagctggct cgcgctatca aacgcgctcg ctacctgtcc
     6121 ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg tccattaata cgactttgag
     6181 aggataaggt aatgcaagtt attctgcttg ataaagtagc aaacctgggt agcctgggtg
     6241 atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag ggtaaagctg
     6301 ttccagctac caagaaaaac attgaattct tcgaagcacg tcgcgctgaa ctggaagcta
     6361 aactggctga agttctggca gcagctaatg ctcgcgctga gaaaatcaat gcactggaaa
     6421 ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc atcggtactc
     6481 gcgacatcgc tgacgctgta actgcagctg gcgttgaagt ggctaagagc gaagttcgtc
     6541 tgccgaacgg cgttctgcgt accactggcg aacacgaagt gagcttccag gttcacagcg
     6601 aagtattcgc gaaagtgatc gtaaacgtag tagctgaata attcgttatt caacgagacg
     6661 taaaaacgcc gaccagtggt cggcgttttg cttttaagcg ataaaaaccg cagaaataga
     6721 cgttccctcc ataaatctct tgctattcct tttataaaaa tttcgcagta cccatacctg
     6781 gcccccagcc tcgccgctgg tagttcgtgt catgtttttt acgcgtaaaa cacgacaaaa
     6841 gcatcaatac agcgatcgtc tcccttgtat gataataaaa acaatatgtt atatatgaat
     6901 gcgctgttat tttattgttt ttatatataa aaggataaaa tatcatcact ttcgtaatct
     6961 tgaacgcttt gcaggtgcgt tcaatccaat aaatgctaat attatggcta ttatagccat
     7021 aaaaagataa aatattatca ctatgggtaa aaaaacagca ccgttactcc cttatacgaa
     7081 tcagatgctt agtgaatttg gtgagcggct gaaacttgct cgtttacgac gcaggcttac
     7141 cgcaaagcaa gttgctgaac gtgctgggat gtcgccaata acattgcgct ctctggagtc
     7201 aggaagtgca ggggtcacga taggggcata tttgtctgtg atgcaggttt tgggcctgga
     7261 aacggattta aataagcttg cagctgaaga cgacttaggg cgtcagctcc aggattcccg
     7321 tttgattaaa aaaagagggc tttctcgttc atcaggtaaa aatgaagaag gtattcagac
     7381 agaccctttt caaacgaggt atgagaaaga aggtttactc accacggatt taacagcact
     7441 ggcagaaggc tcaacgccat ctattacagg agaaacatca acctctcttg cagcactatt
     7501 aaaaccgata aaaaaaccgt taaaaggaaa ttaattatgg cattaatcgg agtgtatgcg
     7561 gattgggaag gcctggatgg tccggagcgg attggttatt tacacagtcg ccgcactcgg
     7621 acgcgtgaga tatttgagtt tgagtacgat aaaaaggcgc ttgcagatcc ttcacttaat
     7681 ttcattcagt tagatcctga aataatgttg tatgagggag cacagtaccc aattcctcca
     7741 aaggataaat ttggtgcatt tagtgattcc tgcccggatc gttgggggcg tatgctaatg
     7801 aagcgtcgtt ttgagaggga tattcgagac gggttatgtg ataaagactc ccacctttat
     7861 gagtctgatt atctactagg tgttcatgat ctttatcggg ttggggctct tcgatacaaa
     7921 cgtgaagacg ctggagagtt tcttgataat cgcattgatg ttgcagcccc accatttacc
     7981 gaaatagcga gtctggaaag ggctagccgg gcaattgaag aagatcctga taataaagaa
     8041 ctaatggggc aaaaatggtt aagaatgcta atcgctccgg ggggatcatt agggggggca
     8101 cgtccgaaaa ccagtgttgt tgatgaagct gggcatctct acatcgctaa atttcccagt
     8161 gtaaaagatg agtatgatgt tggtggatgg gaaatggtcg ttaatgcact tgctgttggc
     8221 tgtggactga atgttgcacc tgctcaggcg cataaatttg ccagcaatta ccattgtttt
     8281 atggttcgtc gttttgaccg cactaatgcg ggaaggcgtc tgcattttgc ttcggctatg
     8341 acactaacca ggcatcagga tggtgaagat acctctacag gtgtgagtta tctggaatta
     8401 gcagatgttc ttatcaggca tggcgcgcag actaatactg atcttaagga actctggtcc
     8461 aggattgttt ttaatattct tgtttctaat accgatgacc atttacgtaa tcatggatat
     8521 atcttgatac cgggaaaagg ttggcggctt tctgaggcat atgatttgaa tcctgttgcc
     8581 aggtctgacg gtctaaaact caatattaca gaaaatgata atgcacttga tttggaatta
     8641 gctcgagaag ttgcggagta ttttcgactt ggtttgacag aagcagatga cattattgct
     8701 aattttagag ggattgtcag tcagtggcga atcattgccg agcgtcttcg attacctggt
     8761 cgtgagcaag agttaatggc agaggcgttc aggggggcaa tttagaatag ttatttcgat
     8821 atattcgcta aatcataaat ttgcgaagca atgccagtag atactgaacg ttttggaaga
     8881 gacgtttaat ttatgagaaa aatagaaacg ctacaaagct aaattatagc gtttctatgt
     8941 aaaataatta gcttgttgct aactgtactt gtggttcttc gtctttaact ttgataagcg
     9001 tccacagcgc aatggcgcca ataatgtcaa agaaagccag ggcaataaag aacggcgcga
     9061 agccaataat gtgaacgaat gccccgataa acaggttgaa aatcaactga ccgctccatg
     9121 cacaggctcc tgccataccc acagcggtag ctacttcgtc ttttttgaat aggtcaccgc
     9181 ccagagttgc tgcaacagta gaaagacatt gatgcgagaa tgcgccaatg ctaatcagta
     9241 atacggcgat gtagggattt tcgacaatac tcaccagacc aatcgtcatc ataatgacgg
     9301 ctgcaatagt gaaggtaatt cttcgtgagt taattaaact cacgccgcga tcgtggaaga
     9361 acttcgcgac aaaaccactg gctaaacagc cgagatcgcc cagcaggaaa ggcagccaga
     9421 cgaacatggc aatttctttc aggctaaaat gcagcgtttc gacgaagaaa atcggtaccc
     9481 agaagttaat ggttccccat gccgggtcag cgagaaaacg cgcgatgcca atcccccaga
     9541 aattacgttg gctgaggata tgcttgattg acgttttttc tttcttatct gttttaagat
     9601 gctgttcttg cccagactca atatagtggc gctcttcatc agaaaggcgt ttagcatctt
     9661 ttggatcttt ataacaaaag aaccaaaata aagccgcgag taaagcaagg ctacctgaga
     9721 caataaacgc aaattgccag ctatgaaaca taatgcacca ggcaatcaac ggtggagcaa
     9781 gcatcgcgcc gagtgaggtg cccatattga aaacacctgt cgccacgcca cgctcttttg
     9841 ccgggaacca tgttgatgcg gtttttacac ccgcaggaat tgccgatgct tcgctgaaac
     9901 ccatcagggc gcgcataaat gccagacctg accatgttcc tgtcaaggca tgccccattg
     9961 tcgccaggcc ccagattgcg gcacatatcg caaaacttaa cttcagaccc acggtatcaa
    10021 taaagaagcc cattatcggt tgccccaatg tataggccaa ctggaatgca ctgacaatcc
    10081 atgaatattg ttctttggtt attccggttg tctccataat cgttggagcc gcaataccta
    10141 acgccgttct gtccagataa ttgattacag tgataaagca tacgagggca atcatatgcc
    10201 atcgataatt ctttatttta ttcattttta aaccttttaa ttatattaat aaaccctggt
    10261 tgttggtatt tccatgtaat aggcaataaa aatcaggtac agaggaaata ttggaaatta
    10321 ttgtgtgtaa atgtctccct ttaaaaagtc tggccagttt ttttcaaagt aaataccgtt
    10381 aatacacggt tgaatacgta aagactttac ttcggtaata aaagaacctt gcgaatattg
    10441 aattgttaat ttgtgatttt tgtcttcagc gagttcagag cgaaaacgcc attcttcttt
    10501 tgcgccgcga cgagtttgtg gtaggcattt gccttgtgga tcaataacca tacgcgcccc
    10561 acggctacca ccaccagcat caatataatg cgttaattgt gttaataccg cggcagaagt
    10621 cagcgccata tgccgccaca taaacagctc ggcaacttca cccacatgtt tgattgccaa
    10681 tccatgccgt tgcacaaatt cactcaatag cagggcatca cgagtggcgc gtcggacttt
    10741 atcggcatgg caaataaatc ccgcatggtc agacattcgt gcctgaattt tttccctcac
    10801 aaccgacaac ggcatcccgg ttccgttatc gtgcgcctga gtaatgatct ctcttataga
    10861 agccagtgcc ggagctgcca gctgtgcaat atctccatca atattccgct tttgcgtgca
    10921 accaataaag cgcgccagac gaacagcaaa aacctgcccg gcattcaatg ccgcaccgcc
    10981 aggtcgggtg acgccgtgtg tgccagcaac ttcccccacc gcaaaacaac cgggcagaga
    11041 tgtttgtccc cagatatcga cttcaatgcc gccattcatg tgctgattat tcatggcaaa
    11101 ctgcaatggc tgcgtgatga gatcgtaacc gtgcatctta tacagcgaga tagacagagg
    11161 attcatttgt tgcaggcgtt cgatgggcga tggtgccaga gcgtcgttat tttccagata
    11221 cgcgcgaacg tcgtcgtcca gtcgatctaa tgagaacggc aggtcacccg gcacaggttc
    11281 tggattacga ttgaaatcca taaatacctg acgaccatgt tgctgctctt gcgctactgc
    11341 catatctaac aagctggagc caaaatccat tacccgcgtg gcgtggaacg gccactgata
    11401 gcctttacgg aaaatgtttg aagccagctc ctgggtggtg cgatagtaat ccgcgaggaa
    11461 gttatactcg ttacccccgg catccacgga atagatatat gggatcacct gtacataagt
    11521 cccggacaaa ttccacggaa acgtgctgcg cggtgtgccg ataccaaact ggctttcagt
    11581 cagattggtt agcgtcagac cttcctccag cgccagcccc agcgagccga aacatttgtg
    11641 tggatacaca ctgtcgcgat aaagctcgcc aggcccgcct gttgccagta ccacattggg
    11701 cgcagtcaca attgccagcc cccatgggtt atgggcgcga tgaccggtcg cgaggattgc
    11761 ccccgccacg cggtcttcgc cgttttcgtc acgctgatgt agcagtttaa tcactgttgc
    11821 actggtcaac attgggatag caagacgctg tacttcttcc agcagcactt tcaccattaa
    11881 ccttgaggta cgcggcccac acgaggttgc acgcccggct tcgtcgtggt cggtttgata
    11941 acgaagaatc gcgccatagc gatcttccgg taattccaga ccaagatatt gcaacccgcc
    12001 aagagtgtgc agagaaccta ccgcttcaac ataagcggtg tcgtgatcca tcgccccacc
    12061 gctcgccagt gcctctgcca gtttggcgaa gttgtcgccg ttgcccgcgg tagcggcggt
    12121 aaacagtgtt tgtttatcag agccagaaca tgccgacgtc cccatatata acccggcggt
    12181 ggcgattaac acattttgct ggcggcgttt aagttccaca gctgcacgta gcccggctgc
    12241 accactgccg actacaaggg catctgtttt ccagagtggg taatcaacac cgtctaccgt
    12301 gacaacccat gcgggttctg cctgtgcaaa accctttggg aaagtaacgt gcgtcagtgc
    12361 atcaaggatg gcctcatcaa gctggctcat gcatcctcca tcggcgtaaa caggctgctt
    12421 tccatcagac gtgggtgctt gataattgga cggaaggcca tgaatggcag aatgtcgcgc
    12481 tcaatctcta cgccaggggc gatctcaatt aattcaacgc cttctgccgt taactggaat
    12541 accgcacgtt cggtgatata cagcacttcc tgaccgcttt gctgtgcgta ggcggcgcta
    12601 aaggtgattt tttccaccag ggcaaccagt ttagggattt gacctgactg ggcgatatgc
    12661 aagccatctg gcgttacatc aatcttgctg cctttggcgt cgaacgtacc gcaaaacacc
    12721 actttacggg cgttttgtgc aatttcgaga aatccgccgg ggccaatgag attgccatta
    12781 agatgggata cgttgacgtt accgtaccga tccatctctc ccatgccgag gaaggtgata
    12841 tcgataccgc cgccactgta gaattcgaat tgatcgagtg atggaataat ggcatcggcg
    12901 ttacgggcgc agccgaacag agcatcatcc agcatcatgc cgttatgcac cccctgttct
    12961 acactcagcc acaaactttg ataaggcacg ccttcgcgca gcgcaacccc aggaataccg
    13021 cccggaatac caaagccaaa gttggttgaa gcacctgcca ccagttcccg cgctgcccga
    13081 cgggcaatca ggcgacgcac cggatgacta accagttcaa tagcgtcgtt agaacttaga
    13141 cggcgatgtt gaccgctgat cgtcaggtcg taaccgccaa gataggtttg cggttgttcg
    13201 cggtgctcaa cgatgccatc gaccagaatt cctggtagct tgacccgacg tggttcaatg
    13261 gtacctgctt ccagcacatc gcggacctgt acgaacactt tgccgccgct gttgtgtgct
    13321 gccagcgcca tcgaatagct atccatatca atcgcttctt cttcgaggct aacgttgcct
    13381 cgtggatcgg cataagtgcc acgcaaaatc gcgtaatcca ctttgaaagg gcgataacga
    13441 agtttggtct caccgtcgat ttcaatcagt tctaccaggt cgtcggttgt gcattcattc
    13501 gacttaccgc cgccattgcg tggatcaaca aacgatccca gcccaacgtg ggtaaaaagc
    13561 cccggacgtc cggcaccgat ctcccgcagc agtgcctgaa ttacgccacc aggaaagcag
    13621 taggcttcaa tcgtgttatt ttttaccagc gcctgcattt tgggcgacca ggtaaagtgt
    13681 ccggcaataa tgcgtttgag catttcggcg tgagcaaaac ggttagtgcc tttgctgtcg
    13741 cgatcgccaa tacccagcga gtggattaat gtcagatcgc gcgggtgtcc ggtttgcaga
    13801 aagcgcgctt caatggcggc cagaatatgg tcagcttcca ccatgccgcc gccgttaccg
    13861 ctaatggcaa tggttgcgcc gtcctgaatt tgcgccgcca gtgtctcggc ggttgataac
    13921 ttacgcatac aatttcctta ttgattgtgg aagctcacgc cggctttttc ggcaaatgcg
    13981 tgcgtcgcct gttggcaatc ctgagtggcg gacattaatc cagccagtaa ctggtgggag
    14041 taatcggcgt gtttattttc cagcgccaga atcagttgct taatgtgtgc cagtgccacc
    14101 ggaccacatt tttccagctg cgctaactgg ttcgccatcc agctttccac ctgttctttt
    14161 tctaccactt catcaattaa attcgccgcc tgagcttcct gcgccgtcag gcgtttaccc
    14221 agcatcagca tctggcgacc gaccaccggg ccaacctgat taagcacgcg ttcaatgccc
    14281 atccagccag gcaccatgcc gagcatcact tcaggattag agaattttgc gtgggggcga
    14341 gcgatacgaa tatcacagca caaggcgagt tcaatcccac cgccaatggt atgaccgttg
    14401 agatttgcaa ccgttaattg gggtaaattg cgcaggcgat taaaaacctc gttaccgcgt
    14461 ttaatccatt ttctgcccat atctaatggc gaataggccg accacgattt aatatcgccg
    14521 cccgcacaaa agaatttatc gccggttgcc gttaattcga ctaatcgtaa tgtggtgtcg
    14581 ttttcaattt catttaaata atggtcgaga gattcgatca tttcttcatt aatagcatga
    14641 catttatctt cacggttcaa tgtcaggcgg caagaagccg ccgctctgct gaacagaacg
    14701 ggttggtcac tcatgattat tccttagcaa taatgcgcga tgcgatcgac gctaatatcc
    14761 agcgtgctgt taatatcttt ctgccaccag tacggtgaga atatttccag ttcaatagcg
    14821 ccattaaatc cggcgttttc aaccagccga cgaattgacg gaatattgat gacaccatct
    14881 cccggcattc cacggtcatt gaccagatcg gtggtcggca ctaaccagtc ggaaacatga
    14941 aacgcgagga tgcgttttcc ggcacgcagg atctgactgg cgagatccgg gtcccaccag
    15001 acatggtaga catctaccgc cacgccgagg ccaaattcgc catcgggatc gagttcatca
    15061 caccagtcca gcgcctgacg taacgtgcac agacaagaac gattggcagc agtcatcggg
    15121 tgcagtggct ccagtgcaat tggcacgccg acctctttcg aatgtggcag caactggcga
    15181 atcccttgtt tcacctgttc ccgtgcttcg tacagatctt ttgttcccat tggcagaccg
    15241 ccaaccacct gcatataaca ggcggcgttc agtacagcgg cgtcgtccag cgcccggcgg
    15301 ttatcgtcaa tcgccagttt gcgctccgcc agcgtcggtg cggtgtagta ggtgctacgg
    15361 cacaaaccgg aaacgttcat gttactggca gcaagctggc gggcgatttg ctggagatct
    15421 tcactctgca actccctgcg ccagggggca atcgcgccaa taccgcgtgc ggcgcaggca
    15481 tcaataatgg cgggtagcgg cgttttatag cccagtgtcg cggtattgat actgaataaa
    15541 tccgggcgtt gtaacagatc tctcatgatt cagcctcaga agcgcggcat acttaagccg
    15601 ccgtcgatgg cgaccgcctg accacaggtg taaataagtt taccttccgc catcgcgcgg
    15661 acagtggaag caatatccgc cggataaccc caacgtcccc acggcaccag acctttggca
    15721 atcagttcgt cgtaataggc cgttgccgga atagtcatat cggttttgat gagaccagga
    15781 cgcacttcat atacgccaat ttgttcatta caaaggcgag cagcaaacag acgagcagcg
    15841 gcagagacgg cagttttggc aatggtatat tcgccgcgat tcatcgccag catgatggcg
    15901 ttgatggaac tgacaaaaat aatcgagcgg tgcggcagtt ctgcttccgg tttcggctgt
    15961 gccagtaagc gcttactgaa tgcctgagca aggaagaacg gtgcgcgggt gttaatggca
    16021 atattttggt cgaagctgtc tggttcgagg tcgagaaggt caccgcgttt tttcactgaa
    16081 atgcctgcgt tgttgagcag gcagtccaga cgtccccact ggttttgcgc tgcgtcgagc
    16141 atctcttcat gcagggagag atcgcccaca tcagcaggga agcaaatcac ttccacgcct
    16201 tccgccagac attcttgttg tgtggcatgt aatttttcta cgctatccgc atccggacga
    16261 tcattaatca gcagattaaa tccgccacgc gcaagctcca gcgcacagcc ctttccaata
    16321 ccacgagcgg ctccggtaat cacggcaaca ggacgagtgg atttcataca atagctctct
    16381 ttttttgaat tttgtttcct tagtgatgat tagcttgcga ctggaggata aaaagatcaa
    16441 ccatctagtt ggttgatatg cgatggcgat cacaggacat ttgataacct gatgaaagta
    16501 caagtttttg cccttttttc tgaagctgaa atcaaatcag gttatgatag tcaccacgaa
    16561 gaggtcgtgg ggaagcgtta tgtatcttga taccagcaac gtacagagcc tgaaagaaaa
    16621 gctgctgtta tgcgcggtta atgaatttgc cgaatatggt tacgaagggg ccagagtcga
    16681 taatatcgtc aaggccgcgg ggtgcagtaa acagacggtt tatcaccact ttggtaataa
    16741 agaaaattta tttatcgaag tactggaata tacctggaac gatattcgac aaaaagaaaa
    16801 ggcccttgat ttctcagatc ttccgccaca gaaagccatt gagaaaataa tcgattttac
    16861 atgggattat tatatttcta atccctggtt tttaaaaatt gtgcatagtg aaaatcaaag
    16921 taagggtgtg cattatgcaa aatcacagcg tcttttagaa attaaccatg cgcacttgca
    16981 gttaatggaa tccttattgg atgaagggaa gaaacaaaat atttttaagc cagatatcga
    17041 cccattgcag gtgaatatta atattgctgc gcttggggga tattatttga tcaaccagca
    17101 tacgcttggc ctggtttatc acatcagtat ggtttcaccg caggcactgg aagccagacg
    17161 taaggtcatc aaagagacaa tccttagctg gcttttggtt gacccttcat ctaccgcgcg
    17221 cgaataaaac tgccatccgg ctggcgggta aacaggacct gttgattatt ccccgtatca
    17281 atggttaagc ccgtcaccac gccgctggcg ttctggcgga ttttcaccat ctgcccgttt
    17341 tgcaaattac tcagtggctt atccgcgcct tctacctgcg ccatggcata gacatcggta
    17401 gcgggcagtc cgtggtcgcg gaacagctgc gccatcgttt taccgggttc gacacgatag
    17461 gagcgccatt gattatcaat tccgctatct ggctggaatg gttgcgtatg ggtggtttgc
    17521 ggctgctctt ctggttggcc ttcctgtatt ggttctggag cgaccggagc cacctgatca
    17581 ggatcatttt gtggcgtgac taattgcgcc cgcagctgtt cttctgtcgg tggttgtgat
    17641 tgcgactgaa tatctaactg tgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca
    17701 gatggaagca gaaatccgac caccaataca atggcggcaa taatgatccc ccgacgatgc
    17761 atcggcggca gcgggtccat gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg
    17821 gttggtttta gttcaaagcg cccgggcatg gtttcctcct gctccgcgtc ttgttcctca
    17881 atcatagcct atgaataagc taacgctatg atgtccgtgg taaacccgcc tttattatat
    17941 tcatacggga tattgctatt gtttcttttt ccccgggatt tgtcatcatt cccgcgacaa
    18001 agttttaccc aaagaagtgt ggctgatatg ctgcccgcta ctttataccc taagaaagga
    18061 aatacgatga ccaccccaac ttttgacacc atcgaagcgc aagcaagcta cggcattggt
    18121 ttgcaggtag ggcaacaact gagtgaatct ggcctggaag ggctgctgcc agaagcactg
    18181 gttgcaggca ttgccgatgc gctggaaggc aaacatccgg ctgttccggt tgatgcggtg
    18241 caccgcgcgc tgcgtgaaat ccacgaacgc gccgatgccg ttcgtcgtca gcgtttccag
    18301 gcaatggctg ctgaaggtgt gaaatacctg gaagaaaacg ccaaaaaaga aggtgtaaat
    18361 agcaccgaat ctggcctgca attccgcgtg atcaaccagg gcgaaggcgc aattccggca
    18421 cgtaccgacc gcgttcgtgt tcattacacc ggaaaactga tcgacggcac cgtgtttgac
    18481 agctccgttg ctcgcggcga gccagctgaa ttcccggtta acggcgtgat ccctggctgg
    18541 attgaagcac tgactctgat gccggtaggt tctaaatggg aactgactat cccgcaggaa
    18601 ctggcatatg gcgagcgtgg cgcaggcgcg tccatccctc cgttcagcac cctggtgttt
    18661 gaagtcgaac tgctggaaat cctctaagca gcgcattctg ttcccctcga accagagggg
    18721 agcaggcatt cagcaataaa cccttcagtt tgccaaacgg cgctatttta tgttgcaaag
    18781 accctgtaag cgtgtatttt tgtgagctgt ttcgcgttat caccgtgata tgacactcac
    18841 tttaaacata aaattaacat tagatctaaa tcttagtatt catcccgcgt attgttacct
    18901 aatatcgatg agtcccgata cagattcgtc gtatcataga ctgactaaag gccgtagagc
    18961 ctgaacaaca cagacaggta caggaagaaa aaaacatggt agatcaggta aaagtcgttg
    19021 ccgatgatca ggctccggct gaacagtcgc tacggcgcaa tcttacaaac cgacatattc
    19081 agcttattgc cattggcggt gccattggta cagggttgtt tatgggggcc ggcaaaacga
    19141 ttagccttgc cgggccgtcg atcattttcg tttatatgat catcggtttt atgctctttt
    19201 tcgtgatgcg ggcaatgggg gaattgctgc tttcgaatct ggaatacaaa tcttttagtg
    19261 acttcgcttc cgatttactc ggaccgtggg caggatattt caccggctgg acttactggt
    19321 tctgctgggt tgtaaccggt atggcagacg tggttgccat taccgcctat gcgcaattct
    19381 ggttccctgg gctttctgac tgggttgctt cgttatccgt gatcattctg ttactggttc
    19441 taaacctcgc cacggtaaaa atgttcggtg agatggagtt ctggtttgcg atgatcaaaa
    19501 tcgtcgccat cgtgtcgctg attgttgtcg gcctggtcat ggtggcgatg cactttcagt
    19561 caccgaccgg tgtggaagca tcatttgcac atttgtggaa tgacggcggc tggttcccga
    19621 aaggcttaag cggcttcttt gccggattcc agatagcggt tttcgctttc gtggggattg
    19681 agctggtagg tacaacagct gcggaaacca aagatccgga gaaatcactg ccacgcgcga
    19741 ttaactccat tccgatccgt atcattatgt tctacgtctt ctcgctgatt gtgattatgt
    19801 ccgtgacgcc gtggagttcg gtagtcccgg agaaaagccc gttcgttgaa ctgtttgtgt
    19861 tggtaggttt gcctgcggct gccagcgtga tcaactttgt ggtgctgacc tctgcggcgt
    19921 cttccgctaa cagcggtgtc ttctctacca gccgtatgct gtttggtctg gcccaggaag
    19981 gtgtggcacc gaaagcgttc gctaaactct ctaagcgcgc agtacccgcg aaagggctga
    20041 ccttctcttg tatctgtctg ctcggcggcg tggtgatgtt gtatgtgaat cccagcgtga
    20101 ttggcgcgtt cacgatgatt acaaccgttt ccgcgattct gtttatgttt gtctggacga
    20161 ttatcctttg ctcgtacctg gtgtatcgca aacagcgtcc tcatctgcat gagaagtcga
    20221 tctacaagat gccactcggc aagctgatgt gctgggtatg tatggcgttc tttgtgtttg
    20281 ttctggtgtt gttgacactg gaagatgaca cccgccaggc gctgctggtt accccgctgt
    20341 ggtttatcgc gctggggctg ggctggctgt ttattggtaa gaagcgggct gctgaactgc
    20401 ggaaataatc gcattatcat gctggatggc gcaatgccat ccagctttta gatcactcac
    20461 ccgccagcgc gcgcgggaat agtacattgt tttccagact gatgtgctcc atcaggtcat
    20521 caatcagttc attaatgccg ttatacatcg ctttccaggt ggtgcaggct tccggcggcg
    20581 gtgtgacgtt attggtggtg tgtttgatca cttccagcag ttcgcccgct tcatcgtgct
    20641 cgctttccat tacgctgatt ggccccattg cctggctgcc catgccttgt ttaatcatcg
    20701 ggaagagaat ttgctcttct ttcatcatgt ggctggaaag ctcttcatgc agcatagtca
    20761 ggtattttgt cagccctttt ggcacacttg gtttgtcggc gtgaacgcgc tcgactttag
    20821 tcgcttgcag gatcagctcc ggcagctgct cgcgatggcg atcgtgatag cgcacaataa
    20881 tgtggtcgat gatttccgcc agcggcgcgc tacgccagtc tttctcaatc ggttgttcag
    20941 caagctttgc cagttcggct tcaatgacgt caacatccag ttctttacgt gccgccgcgc
    21001 gcgccagcgt ctgcttaccg ccacagcagt aatccatatc atatttacga aacagagccg
    21061 aagcgcgagg aatagagagc gccagttcac ctaaaggttg gtcgcgataa gccatagctg
    21121 atacctcatt cttaataaga taagatgcat tttaaatgca tctttaaggc aaaagctata
    21181 acccttacgt agcagaggct tatttaactc actgccagcg tgtcaacttt ctgagacaga
    21241 ttcagttaaa ggtttttctg gttttgctct gaccgcaatg accacgccaa tcactaacag
    21301 cgcgataccg ctcagcgtca ttagcggcgg catttgctgg cggagtaaaa aggtgtacag
    21361 caaacctgcc agcgtttcga aaacaatcag cggcccgaga atcactgtcg gtaatcgctg
    21421 gctggcgacg ttccagcaga gtgcgccacc ccatgagcaa agcacggcta tcgcaaccat
    21481 cagactaata aacaccagcg gacgggggcc aaagggtaag gagaagtccg gcgtttgcat
    21541 attcagccag taacaggcga cgagatagcc gatgagagaa accggcagcg tgaccagcgc
    21601 ctgcgccgtc gcccacatca tcggatgttt gtcgggattt tcccgcagcc agcgggcgtt
    21661 gcgcagggca taccatgccc agcagaccac ggaaactaac gccagcacga tgcctgaggt
    21721 ataacgtgcc cagtcaaaat cggggagtcc gtggtttaac tcagcaatat tcacacacgc
    21781 caggccgatg ccaatacaaa ccagtgccgg ggcgagtttt ccccatgcga gtttgccgtc
    21841 gcgctggcta taaagcagat tggcaaaaac gggaatcacc accggcaggg tgccgataat
    21901 catcgtggaa acaggcgcgc cagtacgttg aatggcactg gcaaggcaga agtaatagat
    21961 aaggttgccc atcatggtga gcatcaaggc ggtaagccag tcgcgacgcg ccaactgacg
    22021 cagacgcacg cgtcccagcc aggcaatggg cagcgcaatt aaccctagcg ccaggtaacg
    22081 ccccatcgac tgtaacatcg ccgggtattc cggcacgatc aacggcccga caaaaataag
    22141 cccccacatc aaccctgcta acagggcgta cagcacgccg ctaatcatca ctggcatcca
    22201 ctggtctgtc agaagaaata ttcaggaagc agacatgatt gctcccggca tcccgtcatt
    22261 atcagagagg atgcgggagg atgccacttt aattaactag ttattaacat taaaaagatg
    22321 gcttacgctt tcggctaacg ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct
    22381 gtcatcaaac agaccgcctt tcgatgcgcc aacgtcagaa tccgccagca tatccgccag
    22441 tccatcaggc agtccgacgc ttttcagcgc cgcagcgaaa tcggcttcgc tcagattttg
    22501 ataggtaacc gattttccgc tctgtttggt gagttctgcc gctaactgtg tcagcgtcca
    22561 ggcactatcg cccgccagtt cgtaaacctt gccttcgtga ccggcttcgc taattacgcg
    22621 tgccgcagct accgcataat cagcccgcgt tgctgaggca attttgccat cgcccgcggc
    22681 accgataaat acgccgtgtt ccagtgctgc cggggcgctg gcgaggtagt tttcggtgta
    22741 ccagccgttg cgcagcaggg tgtaaacgat gccagaatca gccagcattt tctccgtctc
    22801 gatgtgctca tcggcgaggc cgggcgggga ggaatcggca tgtagcaggc tggtataagc
    22861 gataaatttc acgccggccg cctttgcggc attaataaca ttacgatgct gcggggcacg
    22921 ttgacccact tcgctggaag agatcagcag tagtttttcc actccctgaa gcgcagatgt
    22981 cagtgcggct tcatcgccgt agtcagcctg acgtacagta atgccttgcg cgctcaatgc
    23041 ctgcgctttt gccgggttac gtgcgatagc cactatttgg ctggcaggaa cggttttcag
    23101 taaggagtta ataacataat gaccaagttg gccagtggca ccagtaatag cgatcatggg
    23161 aagcctccat cgttttgtct tgtgtttaaa tacgctacca cctaaactta cttttagtaa
    23221 gtacgtacaa aaaggtaagt atgaaatgag tcaggttagc ctgtcgcaac aactcaaaga
    23281 gggaaatctc ttcgctgaac agtgtccgtc gcgcgaggtt ttgaaacacg tcaccagccg
    23341 ttggggggtg ttgattctgg tggcgctacg cgaaggtact catcgcttta gcgacctgcg
    23401 gcgcaaaatg ggcggggtga gtgaaaagat gcttgcgcag tcgttacagg cattagaaca
    23461 ggatggtttt cttaaccgta tcgcgtatcc ggtggtgccg ccgcatgtgg aatatagcct
    23521 cacgccgctg ggcgagcagg tgagcgaaaa ggttgctgca ctggcggact ggattgagtt
    23581 gaatttgccc gaggtgttag cggtgcggga tgaacgtgcg gcataactgc cggatgcgct
    23641 gcgcttatcc ggactacggt tcatggcttg tagggcggat aagatgcgtc atcatcgcat
    23701 ccgccaataa atgccggatg cggttgatga tttgtaggcc gaaaaagaag tgttttactt
    23761 actcagatcc acctgataaa tcgcaaaccc gatatcatcg gtcgcgactt tattcatcgg
    23821 atactgccct ttctctttaa taaacgctgc ggctttatct gacggagagg tttcgaaacg
    23881 gatatccagt ttcttatcgc cagctatcgg tgctaaacgc cagttgttat ctgccgccgg
    23941 gtgaatttcc cccgcacgtt tcgactcatc agcaatccac gctgccagca ccgagcggtt
    24001 ctcatccggt gaagcaaaag cgatatggct gtcgcccgta ccggcaaatt tgccgccgta
    24061 agcgcgatag ttattggtgg caacgaggaa catggcgttc ggatcaatcg gcttgccgtt
    24121 aaaggtcagg ttcttaatcc tttccgcatt ggcattaatc atctggcact cgccgtcata
    24181 acgggcgggc tgggtaacat caatctgata attcacacca tcaataacat caaagttata
    24241 ggtgcggaaa ccatcccagt tgatgagtga ctgtggtttc gtgctgttag gatcaatctg
    24301 gttaaactgt ccggcggagc actccagcca ctctttcacc tctttaccgc tggctttcac
    24361 cacaatcagc gtattggggt agagataaag atcggcggca ttacggaagg tcaactggcc
    24421 tttttccacc tccacatagc ttgcggggtc atttttgcga ccaccaactt taaacggtgc
    24481 ggcagctgaa agcactggca gttttgccag atccggatcg ccctgaatgt aatgctcgac
    24541 atacgctttt tgcgcgttgt tcaccacctg cacggtcgga tcgtcctgca caagcgccag
    24601 atagctatac atattgtcgg cagatttacc gattggcttg ctgacgaact ggcgtgtggc
    24661 atcgtgatcg gctttgagtg tttctaccag cttgctgtct tccgccgcga gggatttttt
    24721 attcgcgatg tcgtaaatcg gccgtgcttc cgctttcgcc tgcgtcacct gccatttacc
    24781 gctgtcatta ctgagttgta agtcgaccac accaagatga tcgccccaca tgcctggcat
    24841 taccgccgga acaccattca gcgtgccttt cgcgatatca gccccttcga tatcagcaaa
    24901 atctttacct gggaaaacgg cgtgagcatg gccaaacata atggcgttaa cgcccggaat
    24961 ttcactgagg taataaactg agttttccgc catcacttta tacggattgg cagacagccc
    25021 ggaatgcgcc agaacgacaa cgacatcggc acctttctcg cgcatttcag gcacgtattt
    25081 gcgcacggtt tcggtaatat cattcaccgt cactttcccg gataaattag ctttatccca
    25141 gcccatgatt tgtggcggga cgacgccaat ataaccaatc ttcagcgtct gtttttttcc
    25201 gtctttatcg accacttcgg tgtctttaat taaatacggt gtaaacattg gctgtttggt
    25261 tctggcgtca atgacgttgg catttacata agggaatttc gctcccgcca gcgcattttt
    25321 cagataatcc agaccgtagt taaactcgtg gttgccaagc gtgccgacgg tatagtccag
    25381 cgtatttaat gccttataga ccgggtgaat atcacctgct tttaatcctt tcgccgacat
    25441 gtaatctgcc agcggactcc cctgaatcaa atcgccgtta tcgaccagta cgctgttttt
    25501 cacttcattg cgggcatcgt taatcaggct tgccgtacgt accagtccga atttttccgt
    25561 ggcggtgtct ttgtaataat cgaaatccat catgttgcta tgcagatcag tggtttccat
    25621 gatacgcaga tcgaccgtcg ctgcattcac actggcggca atcagcgtgg ccaggagcgt
    25681 tgcgctaaac ttaatcatca gggacatcct tttatcatcg ggaatacgaa agaaaaggga
    25741 gaataaacgt cttacttata gaacagtgaa gaatgccaca attttacgct ttgaaaatga
    25801 tgacactatc acagttggcg cattcattaa cgatagggta taagtaaaac aataagttaa
    25861 caccgctcac agagacgagg tggagaaatg ttagatcaag tatgccagct tgcacggaat
    25921 gcaggcgatg ccattatgca ggtctacgac gggacgaaac cgatggacgt cgtcagcaaa
    25981 gcggacaatt ctccggtaac ggcagcggat attgccgctc acaccgttat catggacggt
    26041 ttacgtacgc tgacaccgga tattccggtc ctttctgaag aagatcctcc cggttgggaa
    26101 gtccgtcagc actggcagcg ttactggctg gtagacccgc tggatggtac taaagagttt
    26161 attaaacgta atggcgaatt caccgttaac attgcgctca tcgaccacgg caaaccgatt
    26221 ttaggtgtgg tgtatgcgcc ggtaatgaac gtaatgtaca gcgcggcaga aggcaaagcc
    26281 tggaaagaag agtgcggtgt gcgcaagcag attcaggtcc gcgatgcgcg cccgccgctg
    26341 gtggtgatca gccgttccca tgcagatgcg gagctgaaag agtatctgca acagcttggc
    26401 gaacatcaga ccacgtccat cggctcttcg ctgaaattct gcctggtggc ggaaggacag
    26461 gcgcagctgt acccgcgttt cgggccaacg aatatttggg ataccgccgc tggtcatgct
    26521 gtggctgcag ctgccggagc gcacgttcac gactggcagg gtaaaccgct ggattacact
    26581 ccgcgcgagt cgttcctgaa tccggggttc agagtgtcta tttattaatt aatttatgca
    26641 gcaggtccat cacttgctgc acctcttcct gagtaagcgc cccgtctttg gcccattgca
    26701 cgcgaccgtc tttatccagc accactacag cggaactttt ttcatccagc tgccaggcac
    26761 cgcgtgcgac gccattgcta tcaacaataa actgcgacca gggataaagc tttttattac
    26821 tctccagact gctgcgcaca aacatccctg aacccggaat cgcgtcgtcg gtgttaacaa
    26881 tggtggtggt ctggtaacga tcgtgcgata acttcgctga tttaatcgct tcaatcagcg
    26941 tcgcgttttt ctcttttgca gaggtgcgac cagcaatatg ttgcagtact cgcacttttc
    27001 ccactaactg cgcgctgttc caggttttgt agctaaactg atctttatca agcaccaact
    27061 cgccacgatc ggtgatgcca atcggcggca ctcgctgacc ggtttcgaac tgatgtgcgg
    27121 aagccatcat cggcaacagc aggcaggtga gtgccagaat cttgcgtagg gtcatggtgt
    27181 ttccttctta tgatatgcag gtgatccgac cacttgggtc tgagttttaa tcatatgtgc
    27241 tatttatcga atttcccgca agtgtgatgc cagtttgcgg tcaagcgcac aaatcatatg
    27301 aaaaatgaat gcttatactg aagaccgcgc ttcggtaaaa agataattct gaataattgt
    27361 aacctttagg taaaaaaagt tatacgcggt ggaaacattg cccggatagt ctatagtcac
    27421 taggcattaa aatttgcgcc tcataatagt tgggccgatt gtggcaccgc aaaggcgtaa
    27481 tactcagcag gagataacaa tgaaaatttt ccaacggtac aacccacttc aggtggcgaa
    27541 gtacgtaaag atcctgttcc gtggacggtt atacatcaag gacgttggcg cttttgaatt
    27601 tgataagggt aagattctta tcccaaaagt gaaggataag ctgcatttgt cagtgatgtc
    27661 cgaagttaac cgtcaggtta tgcgtctgca aacagagatg gcttaaccaa agtgctatgc
    27721 agtaaaaagt gctatgcagt aataagacgg ctcctgattc aggagccgtt gatgtttctg
    27781 gggttatgcg acgctttctt ctttatcttt agcgtcaggc agttttggcg aaagggcggt
    27841 ggctttgctg tcgattcggg tcaccagcag ctggtcgatg cggtagttat cgatatccac
    27901 cacttcaaat ttgtagccgg cgaatttcac cgaatcggtg cgtttcggga tcttacgcag
    27961 cataaacatc ataaagccgc cgatggtttc gtagttgccc gactgcggga actcgtcaat
    28021 atccagcacg cgcatgacgt cgtcaatagg cgtgccgccg tctatcagcc atgagttctc
    28081 atcacgggcg acaatttgct cttccagccc ctggccgacc agatcgccca tcagtgtggt
    28141 catcacgtcg ttgagggtga tgatccccac taccagcgcg tactcgttca tgatcaccgc
    28201 gaagtcttca cctgcggttt taaaactttc caacgcctct gaaagggtta acgtatccgg
    28261 cacaatcagc gtgttgcgaa tttgtacgcc gctgttcagt gccaggcttt ggttagccag
    28321 cacgcggttc agcaggtctt tagaatcgac atagccgatg atgtggtcaa tatcttcatt
    28381 acagacgaga aacttagagt gcggatgttc cgcaacctta ttcttcagac tttgctcatc
    28441 ttcgtggaga tcaaaccaaa tcacgttttc acgcggtgtc attgaagacg gaacggtacg
    28501 ggattccagc tcaaagacgt tttcaatcag ctcgtgttcc tgtttacgta acacgcccgc
    28561 cagcgcaccg gcttccacta ccgcgtagat atcatcagaa gtgatgtcat ctttacgtac
    28621 cattggcagt ttgaaaatac ggaagattat gttcgccagt ccgttgaaga accacaccag
    28681 cggggtgcaa acgtacaggc agaagcgcat cgggttgatg atacgcaaag ccacagcttc
    28741 tggcgcaatc ataccgatgc gtttcggggt taaatccgca aacaggataa acatgccagt
    28801 cactaacgag aaagagagaa taaagctcag ttgctcagag agttctgccg acatatagcg
    28861 ggagaacagg ctgtgaaaag ctggagaaaa tgccgcatca ccgacgatac cgccgagaat
    28921 cgccactgcg ttcagaccga tctggaccac ggtaaagaac atgccgggat tttcctgcat
    28981 attcaaaacg cgttgggcat ttatattgcc ttcatcagcc agcagtttaa gtttgatttt
    29041 gcgtgaggcg gcaagcgaga tctcggacat cgagaagaac gcacttacag cgatcaaaca
    29101 gagtatgact aaaatactgt ttaacatatc ttatccgacc tttcaggcca gatcctcgga
    29161 aggggaagtt gattaatttg tgtgaaatac acattgaaag ccgattcgaa gaaagtgaac
    29221 cggcattttc agtggctagt atagcgtaag gtactgtaaa gccgccagag ggttaaaatt
    29281 cagacagctg aaaaatgaaa aactgcctga tgcgctatgc cgaacaggcc tacgcagtcc
    29341 ccgcaatata ttgaatttgg acgattttgt aggcctggca ttttgtttac gccgtatccg
    29401 gcataaacaa aatgcacttt atcaacaatc tacatttgta cccgtaacgc tatgcttccg
    29461 gcggcagaca gacgccaatt ccgccaattc cacagtaacc atacgggttt ttatgcagat
    29521 attgctggtg gtcatcttcg gcataataaa acggtgtggc gttagcgatt tccgtggtga
    29581 tgcgacgatc gtcatcggcg gcaagcatcg ccgcctgaaa acgttccaga ctggcgcgag
    29641 cggcggcatc ctgttctggg gtcagcggat aaatcgctga acgatactgc gtgccgtggt
    29701 cattgccctg acgcattccc tgggcgggat cgtgattctc ccaaaatact tgtagcaact
    29761 gctcatagct gatgacggat ggatcgtaaa caatgcgcac cgcttcggca tggccagtat
    29821 cgccggagca cacttcccga taagtcggat ttggcgtata gccgccggta tagcctgcgg
    29881 cggtgctgta aacgccgggt aactgccaga acagacgctc cacaccccag aaacaaccca
    29941 tcgcaaaaat ggcaatctcc attccgtcag gtacattggt cattgagtgg ccgttgaccg
    30001 catgcagtgt ggctacgggc atcggggtgt tacgtccagg cagggcatcg gcgggggaaa
    30061 ccagatgctt tttatcaaat aaactcatgg tgtcgctctc ccgaaaatcg gtcattgggg
    30121 ttaaggttgt aacaagaggc gtatttgcac acaataacca ccgtgaatag gtctaaagta
    30181 aaacataaga aatattttgg gtttagtctg ctttttaatc catattactg gatttttgtt
    30241 aagccgttta acggcgttcc aggggcagga aaaaaggata ttcaggagaa aatgtgcgct
    30301 atatccgaca gttatgctgt gtaagcttac tctgcttaag cggatctgcc gtcgccgcga
    30361 acgtccgtct acaggtcgag gggttatcgg gacagctgga aaagaacgtt cgcgcgcagc
    30421 tttctacgat tgaaagtgat gaagtgacgc cagaccgtcg ctttcgcgca cgcgtcgatg
    30481 atgccatccg cgaaggtctg aaagcgctgg gctattacca gccgaccatt gaatttgatc
    30541 tccgtccacc gccaaagaaa gggcggcagg tattgatcgc caaagtcacg ccaggcgtgc
    30601 cggtgttaat tggcggcacc gatgtggtat tgcgcggcgg cgcgcggacc gataaagact
    30661 atttgaaatt gctcgatact cgcccggcta ttggtacggt actgaaccag ggcgattatg
    30721 aaaatttcaa aaagtcctta accagcattg cgttgcgtaa aggttatttc gatagcgaat
    30781 ttaccaaagc gcagctgggc attgcgctcg gcctgcataa agccttctgg gatattgatt
    30841 ataacagtgg cgaacgttac cgctttgggc atgtgacctt tgaaggatca caaatccgcg
    30901 atgaatacct gcaaaatctg gtgccgttta aagagggcga tgagtacgaa tcgaaagatc
    30961 tggcagaact gaaccgtcga ctttctgcta ccggctggtt taactcggtg gtggtggctc
    31021 cacaatttga taaagcgcgc gaaacgaaag tattaccatt gacgggcgtg gtttcgccgc
    31081 gaacagaaaa caccatcgaa accggcgtcg gttactctac ggacgtggga ccgcgcgtga
    31141 aagcgacgtg gaaaaagccg tggatgaact cttacggtca cagtttgacc accagtacca
    31201 gtatttccgc gccggaacag atcctcgact tcagctataa aatgccgctg ctgaagaatc
    31261 cgctggaaca atattatttg gtgcagggcg gttttaagcg cactgacctg aacgataccg
    31321 agtctgactc cactacgctg gtggcttctc gctactggga tctctccagc ggctggcagc
    31381 gtgccattaa cctgcgctgg agtttcgacc actttaccca gggtgaaatt accaacacca
    31441 cgatgctgtt ttatcctggg gtgatgatta gccgcacgcg ttctcgtggt ggcctgatgc
    31501 caacctgggg cgactcgcaa cgctactcta tcgactactc caacacggcc tggggctcgg
    31561 atgttgattt ctccgttttc caggcgcaga acgtctggat ccgcacactg tacgatcgcc
    31621 atcgttttgt gacacgcggc acgctgggct ggattgaaac cggtgatttc gacaaagtac
    31681 cgccggatct gcgtttcttc gccgggggcg atcgcagtat tcgcggctac aaatacaaat
    31741 ctatcgcccc gaaatacgcc aacggtgacc tgaaaggggc ctcgaagctg ataaccggat
    31801 cgctggaata ccagtacaac gtgaccggaa aatggtgggg cgcggtgttt gtcgatagcg
    31861 gcgaagcggt aagcgatatt cgccgcagcg actttaaaac cggtaccggt gtcggcgtgc
    31921 gctgggaatc gccggtcggg ccaatcaaac tcgattttgc cgtgccggtc gcggataaag
    31981 acgaacacgg gttacagttt tacatcggtc tggggccaga attatgagtt tatggaaaaa
    32041 aatcagcctc ggcgtggtta tcgttatctt actgttgctg ggatcggtgg cgtttctggt
    32101 gggcaccacc agcggcctgc atctggtatt taaagcggcg gatcgctggg tgccaggact
    32161 ggatattggc aaggtcaccg gcggctggcg tgatctcacc ttgtctgacg ttcgttatga
    32221 gcagccaggc gtggcggtaa aagcgggcaa tctgcatctg gcggtcgggc ttgagtgcct
    32281 gtggaacagt agcgtttgta ttaatgatct ggcgctgaaa gacattcagg tcaacatcga
    32341 cagtaaaaaa atgcctcctt ctgaacaggt tgaagaagag gaagatagcg gtccgctgga
    32401 tctctccacg ccgtatccca ttaccctgac acgggtggcg ctggacaacg tcaacatcaa
    32461 gattgatgac accacggtat cggtgatgga cttcacctcc ggcctcaact ggcaggagaa
    32521 aaccctgacc ctgaaaccga catcgctgaa aggcctgttg attgctttgc cgaaagtggc
    32581 ggaagcggcg caggaagaag tggtcgaacc gaaaattgaa aatccacagc cggaggaaaa
    32641 gccgctcggc gaaacgctga aagatctctt ttctcgcccg gtattgccgg aaatgaccga
    32701 cgtgcatttg ccgcttaacc tgaacattga agagtttaag ggcgagcagc tgcgcgtgac
    32761 aggcgacacg gacatcaccg tgagcaccat gctgctgaaa gtgagcagca ttgacggcaa
    32821 tactaaactg gacgccctgg atatcgactc cagtcaaggg atcgtcaacg ccagcggcac
    32881 ggcgcagctg tcagacaact ggccggtgga tatcaccctc aacagcacac tgaacgtgga
    32941 gccgttgaaa ggtgaaaagg tgaagctgaa agtgggcggt gcgctgcgcg aacagctgga
    33001 gataggcgtt aacctttccg gtccggtgga tatggattta cgcgcccaga cgcgactggc
    33061 ggaagccgga ttgccgctca acgtggaagt gaacagcaaa cagctttact ggccgttcac
    33121 tggtgagaag cagtatcagg cggatgatct gaaactgaaa cttaccggca aaatgaccga
    33181 ttacacgctc tctatgcgca cggctgttaa gggccaggag atcccgccag ccaccattac
    33241 ccttgatgcc aaaggtaatg aacagcaggt caatctcgac aaacttaccg tcgcggcgct
    33301 ggaagggaaa acggaactta aggcgttgct cgactggcag caggccatta gttggcgcgg
    33361 tgagctaacg cttaacggca ttaacaccgc caaagagatc ccggagtggc cgtcgaaact
    33421 caatggcttg attaaaaccc gcggtagcct gtacggcggc acctggcaga tggaggtgcc
    33481 agaactgaag ctgaccggta acgtcaaaca gaacaaagtg aacgttgacg gcacgctgaa
    33541 aggcaacagt tatatgcagt ggatgatccc agggctgcat ctggaactgg ggccaaacag
    33601 tgccgaagtg aaaggcgagc tgggggtaaa agatctcaat cttgatgcca ccatcaacgc
    33661 gccggggctg gataacgcgc tgccggggct tggcggtaca gcgaaagggc tggtgaaagt
    33721 acgcggcacg gtggaagcgc cacaactact ggcagatatc accgcgcgtg gcctgcgctg
    33781 gcaggaactt tccgtggcgc aggttcgcgt ggaaggcgac attaaatcca ccgatcagat
    33841 cgccgggaaa ctcgacgtac gcgttgagca aatttcgcaa ccggatgtaa atatcaacct
    33901 cgtcaccctg aatgccaaag gcagtgaaaa gcagcacgag ctacagttgc ggattcaggg
    33961 cgagcctgtc tccgggcagc ttaatctggc aggaagtttt gatcgcaaag aagaacgctg
    34021 gaagggaact cttagcaata cccgcttcca gacgccggtc ggcccgtggt cgctgacccg
    34081 cgatattgcg ctggattacc gcaataagga gcaaaaaatc agcatcgggc cacactgctg
    34141 gcttaacccg aatgcggaac tgtgcgtgcc gcaaactatc gatgcgggtg ccgaagggcg
    34201 tgcggtggtg aatctcaacc gcttcgacct ggccatgctg aaaccattta tgccagaaac
    34261 cactcaggcc agcggtatct tcacgggtaa agcggacgtt gcctgggaca ccacgaaaga
    34321 ggggctgccg cagggcagta tcactctttc ggggcgtaat gtgcaggtga cgcaaaccgt
    34381 caacgatgcg gcgctgcccg tggcgtttca gacgctgaat ctgacggcgg agttgcgtaa
    34441 caaccgtgcc gaattgggct ggaccatccg cctgaccaat aacggccagt ttgatggtca
    34501 ggtgcaggtg accgatccgc aaggccgccg taatcttggt ggcaacgtca atatccgtaa
    34561 cttcaacctt gcgatgataa accccatctt tacccgtggg gaaaaagcag cggggatggt
    34621 gagtgccaac ttgcgtctgg gtggtgatgt gcaaagcccg cagttgtttg gtcagcttca
    34681 ggttacgggg gtggatatcg acggcaactt tatgccgttt gatatgcaac caagccagct
    34741 tgcggtcaac tttaacggta tgcgctcgac gcttgcaggt accgtgcgga cccagcaggg
    34801 tgaaatctac ctgaacggtg atgccgactg gagccagatt gaaaactggc gggcgcgggt
    34861 aacagcgaag ggcagtaaag tgcgtatcac cgtgccgccg atggtacgaa tggatgtatc
    34921 gccagatgtt gtattcgagg ctacaccaaa cctgtttacc ctcgatggtc gcgtggatgt
    34981 tccgtgggcg cgcatcgtgg tgcacgatct gccggaaagc gcagtaggcg tttccagcga
    35041 tgtggtgatg cttaacgata acctgcaacc ggaagagccg aaaacggcgt cgattccgat
    35101 taacagtaac ctgattgtcc acgttggcaa caatgtgcgc attgacgcct ttggcctgaa
    35161 agcgcggctg acgggcgatc tcaacgtcgt tcaggacaaa caagggctgg gcctgaacgg
    35221 gcagatcaac atccctgaag ggcgcttcca tgcctatggt caggatctga ttgtgcgtaa
    35281 gggtgagcta ctgttctctg gtccgccaga tcaaccgtat cttaatatcg aagctattcg
    35341 taacccggat gctacagaag acgacgtcat cgccggagtt cgcgtcactg gtctggcgga
    35401 cgaaccgaaa gcggagatct tctctgaccc ggcgatgtcg caacaagctg cattgtctta
    35461 tttgctacgt ggacaagggc tggagagcga tcagagcgac agtgcggcaa tgacctcgat
    35521 gctgattggc ctgggggttg cgcaaagtgg ccagattgtg ggtaaaatcg gcgagacgtt
    35581 tggcgtaagc aatttagcgc tcgacaccga gggagttggc gactcctccc aggtggtggt
    35641 cagcggctat gtattgccag gcctgcaagt gaaatacggc gtgggtatat ttgactctat
    35701 agcaacactc acgttacgtt atcgcctgat gcctaagcta tatctggaag ccgtgtctgg
    35761 tgtagaccag gcactggatt tgctctatca gttcgagttt tagcaatgcg aatatttgtc
    35821 tacggcagtt tacgccacaa acaaggcaac agtcactgga tgaccaatgc ccagttactg
    35881 ggcgatttca gtattgataa ctaccagttg tatagcctgg gccactatcc aggcgcagtt
    35941 ccggggaacg gaacagtaca tggtgaagtt tatcgtattg acaacgccac gctggccgaa
    36001 cttgatgcct tgcgcaccag gggcggtgaa tacgcgcgcc agttgattca gacgccgtac
    36061 gggagtgcat ggatgtacgt ttatcaacga cccgtcgatg gattaaagct aattgaaagc
    36121 ggcgactggt tagacaggga taagtaataa taaaagcacg ccaccttcgg gtggcgttgt
    36181 tttatccacc agcctgaata tattccgaat ttgacgccat tcttatcttt tcttaaatgc
    36241 tgatttgttg cgcagtgtta taaacacctg gcacgctcaa caaggacgcg accacaatga
    36301 aaaaatctct tctggctgta atgctgacag gactgtttgc tctcgtttct cttcccgctc
    36361 tgggaaatgt caatctcgaa caattaaagc aaaaagctga aagtggagaa gctaaagcac
    36421 agttggagct gggatatcgc tattttcagg gtaatgaaac gacaaaggat ctcacccagg
    36481 cgatggactg gtttcgccgt gccgcagagc agggatacac cccggcagaa tatgtactgg
    36541 ggttacgcta tatgaacggt gaaggtgtgc cgcaagatta tgctcaggca gttatctggt
    36601 acaaaaaagc ggcactgaag gggcttccgc aagcgcagca gaatctgggt gtaatgtacc
    36661 atgaaggtaa tggcgtgaag gttgataaag ccgaatctgt gaaatggttt cgcttggcag
    36721 cagagcaagg tcgtgacagc ggccagcaaa gtatgggaga cgcatatttt gaaggcgatg
    36781 gtgtgacgcg ggattacgtt atggcacgtg agtggtatag caaagcagcg gaacaaggta
    36841 acgtctggtc ctgtaaccag cttggttata tgtattccag aggtttaggc gttgaaagaa
    36901 acgatgccat atcggcacaa tggtatcgaa aatcagcgac atcaggcgac gagttggggc
    36961 agcttcattt agccgatatg tactatttcg gtatcggtgt tactcaggat tacactcagt
    37021 cacgggtatt attttcccag tcggcagagc agggaaattc tattgcgcag tttcgtctgg
    37081 gatatatatt ggagcaaggt ttagcgggag cgaaagagcc gttaaaagca ctggagtggt
    37141 accgtaaatc ggcagaacag ggaaattccg atggtcagta ttatttggca catctgtatg
    37201 ataaaggcgc agaaggtgta gcaaaaaatc tagaacaagc catctcctgg tacacgaaat
    37261 cggcagaaca gggggatgct accgcacagg ctaatctcgg cgctatttac ttcagacttg
    37321 gttcagaaga agaacataaa aaggcggtgg aatggtttcg caaggccgcc gcaaagggtg
    37381 agaaagcggc gcaatttaat ttgggtaatg ctttactcca gggcaaaggt gttaaaaaag
    37441 atgagcaaca ggccgcaatc tggatgcgaa aagccgcaga gcaaggatta agtgcagcgc
    37501 aggtacaatt aggtgaaatc tattattatg gcttgggcgt agaacgtgat tatgtgcagg
    37561 cctgggcgtg gttcgatacc gcatcgacca atgatatgaa tctttttggt acagaaaacc
    37621 gcaacattac agagaaaaaa ctgacagcca aacaactgca acaggctgaa ttattatcgc
    37681 aacaatatat agaaaaatat gccccggaag cctgggcgag aatgcaaaag cttaaagcgc
    37741 aatcagcggt aaagacgggt aataaataac gggtaactgt cagatcaggt ctgcgctaat
    37801 acagctcaac caatgccacc tgcgggtggc gttatttttt tgcgagacga cgcgtattct
    37861 tttttgtaac ttcctcgcct tttgcttttc cttccgagcc gctttccata tccgttaacg
    37921 catataaaac tttgctggcg ttcacaatta ctcaggggta aaacgggtgc acgctttgaa
    37981 cggtgagtat gagtgaggcc ggctaacttc cagcaagtgg ctatgaacag caaaatggtg
    38041 gtcgagttta gcttgttctt accgttcaag cctttaaact gctgaaaata ataatagtgg
    38101 agtagagagt ttataaattg cgggcataaa aaaaccccta ctaaccgaag cccggcgttc
    38161 agggttatta cgctagaaga acttatttat tctttgcgcg ctcgaaggag gcaacgattt
    38221 cagctttagc ggcttctgcg ttttcccaac cttcaacttt aacccacttg cctttttcga
    38281 ggtctttgta gtgctcgaag aagtgagcga tttgcgcttt cagcagctct ggcagatcgt
    38341 taacgtcttt aatgtgatcg tattctttgc tcagcttggt gtgcggaaca gcaatcagtt
    38401 tcgcatcttc accggcttcg tcggtcattt tcagtacgcc aaccggacgg cagcggatca
    38461 cagaacccgg ctgcagcggg tacggagtcg ggaccagtac gtcaaccggg tcaccgtcca
    38521 gagacagggt gtggttgatg taaccgtagt tgcacggata gaacatcgcg gtggacatga
    38581 agcggtcaac gaacagtgcg ccgctctctt tgtcgatttc gtatttgatc ggatctgcgt
    38641 tagccgggat ctcaataaca acgtagatgt cttccggcag atctttaccc gcagggacgt
    38701 tgagtaagct catgtctgtt tcctttaaaa atatgtggca aacaagtgcc gagtattata
    38761 gccaactcgc gccgaatgtc ttcgcttgtt ttcgtcttca ttttcctttt tcgccagttt
    38821 taagacggta tacagagcag gaaaatccat aaccgtagcc gcatttttca tagtgagatg
    38881 aaagcgatta caaacttgtg attaacgttt tatttacttt tttgaagtgt gatgtaacgc
    38941 aatccgttac ataacgaatt gtctatagtt ttttcgcgaa catcttttca ccaataataa
    39001 ttaccccgac gaggacaacc ctatgtggaa acgcttactt gtagtctctg cagtctcggc
    39061 agccatgtcg tctatggcgt tggccgctcc attaaccgta ggattttcgc aggtcggatc
    39121 ggaatccggc tggcgcgccg cagaaaccaa tgtggcgaaa agtgaagccg aaaagcgcgg
    39181 aattacgttg aaaattgccg atggtcagca aaagcaggaa aaccagatta aagcggtacg
    39241 ttccttcgtc gcgcaagggg tggatgcgat ctttatcgct ccagtggtag cgaccggttg
    39301 ggagccggta ttaaaagagg cgaaagatgc cgaaatcctg gtcttcttgc ttgaccgttc
    39361 catcgatgtg aaagacaaat ctctctatat gaccactgtc accgccgaca acatcctcga
    39421 aggcaagttg atcggtgact ggctggtaaa agaagtgaat ggcaaaccat gcaacgtggt
    39481 ggagctgcag ggcaccgttg gggccagcgt cgccattgac cgtaagaaag gctttgccga
    39541 agccataaag aatgcgccaa atatcaaaat tatccgctcg cagtcaggtg acttcacccg
    39601 tagtaaaggc aaagaggtca tggagagctt tatcaaagcg gaaaacaacg gcaaaaacat
    39661 ctgcatggtt tacgcccata acgacgacat ggtgattggt gcaattcagg caattaaaga
    39721 agcgggcctg aagccgggca aagatatcct gacaggctct atcgacggcg tgccggacat
    39781 ctacaaagcg atgattgatg gcgaagcgaa cgccagcgtt gaactgacgc cgaacatggc
    39841 aggccccgct tttgatgcgc tggagaaata taaaaaagac ggcaccatgc ctgaaaagct
    39901 aaccctgacc aaatccaccc tttatctgcc tgataccgca aaagaagagt tagagaagaa
    39961 gaaaaatatg gggtattgag ggttgctatg cctgatgccg attcgtaggc cggataaggc
    40021 gctcgcgccg catccggcgg tggtgcgctg aagcctgatg cgacgcttac cgcgtcttat
    40081 catgcctact gggagcacgc tttacaccgg gggaaaccat gacgaccgac caacaccagg
    40141 aaatcctccg caccgaagga ttaagtaaat ttttccccgg tgtcaaagcg ttagacaacg
    40201 ttgatttcag cctgcgccgt ggcgaaatca tggcgctgct cggtgaaaac ggggcgggaa
    40261 aatcgacgct aatcaaagca ttaaccggtg tataccacgc cgatcgcggc actatctggc
    40321 tggaaggcca ggctatctca ccgaaaaaca ccgcccacgc acaacaactc ggcatcggca
    40381 ccgtctatca ggaagtcaac ctgctaccca atatgtcggt cgctgataat ctattcatag
    40441 gtcgcgaacc caaacgtttc ggccttctac gccgtaaaga gatggaaaag cgcgccaccg
    40501 aactgatggc atcttacggt ttctccctcg acgtgcgcga accgctcaac cgcttttcag
    40561 tcgcgatgca gcaaatcgtc gccatttgcc gggctatcga tctctccgcc aaagtgctga
    40621 tcctcgatga acccaccgcc agtctcgaca ctcaggaagt agagttactg tttggcctga
    40681 tgcgtcagct tcgcgatcgc ggcgtcagcc tgatcttcgt cactcatttt ctcgatcagg
    40741 tttatcaggt tagcgatcgg atcaccgtct tacgtaacgg cagttttgtg ggctgtcggg
    40801 aaacccgcga gctaccgcag atcgaactgg taaaaatgat gctggggcgt gaactggaca
    40861 cccacgcgct tcagcgtgcc gggagaacat tgctgagcga caaacctgtt gccgcgttca
    40921 aaaattacgg caaaaaagga acgatcgcgc cgtttgatct cgaagtacgc cccggcgaga
    40981 tcgtcggtct ggcaggcttg ctgggatcag gacgtaccga aaccgccgaa gtgatcttcg
    41041 gtatcaaacc tgctgatagc ggcacggcgt tgatcaaagg caaactgcaa acgctacgct
    41101 cgccacatca ggcttcggta ctgggcatcg gcttctgccc ggaagacagg aaaaccgatg
    41161 gcattatcgc cgccgcctcg gtgcgggaaa atatcatcct cgctctccag gcccagcgcg
    41221 gctggctgcg accaatttcc cgcaaagagc agcaagagat tgccgaacgc tttattcgcc
    41281 agcttggcat tcgcacacct tccaccgaac aaccgattga atttctctcc ggcggcaatc
    41341 agcaaaaagt gttgctttca cgttggctac tgacccgacc gcaatttctg attctcgacg
    41401 agccaacgcg cggaattgat gttggtgccc acgccgagat catccgcctg attgaaacgc
    41461 tgtgcgccga tgggctggcg ctgctggtga tctcctccga gctggaagag ctggtgggct
    41521 atgccgaccg ggtgattatc atgcgcgatc gcaaacaggt agcggagatc ccactggcag
    41581 agctttccgt tccggcgatc atgaacgcca ttgcggcgta aggagaacag tgtgatgcct
    41641 caatctctcc cggacactac gccgccgaaa aggcgttttc gctggccaac aggaatgccg
    41701 cagctggcgg cactgttgct ggtgctgctg gttgatagcc tggtggcccc gcatttctgg
    41761 caggtggtgc ttcaggatgg gcgtttgttc ggtagcccta tcgacatttt taaccgtgcg
    41821 gcccccgttg cgctactggc gattggcatg acgctggtta tcgccaccgg tgggattgat
    41881 ctctccgtag gggcggtgat ggctatcgcc ggagccacaa cggctgcgat gacggtcgcg
    41941 ggattcagcc tgccaattgt tttgttaagc gccctgggca ctggcatcct ggcgggattg
    42001 tggaacggca tactggtagc gatcctcaaa attcagccgt ttgtcgccac cctgatcctg
    42061 atggtcgccg ggcgcggcgt ggcgcaactg atcacctccg gacagatcgt cacatttaac
    42121 tcaccggatc tctcctggtt cggcagtgga tcgctgttgt tcctgccaac gccagtcatt
    42181 attgcggtgc tgacgcttct cctgttctgg ctgttgaccc gcaaaacggc actggggatg
    42241 tttatcgaag ccgttggtat caacattcgg gcggcaaaaa atgccggggt aaacacgcgg
    42301 atcatcgtca tgctcaccta cgtgttgagc gggctatgtg cggcgattgc gggcattatc
    42361 gtggcggcgg atattcgcgg tgccgatgcc aacaacgccg gcttatggct ggagctggac
    42421 gccattcttg cagtggtgat tggcggcgga tcgctgatgg gcgggcgctt taacctgctg
    42481 ctttcggtgg tgggggcgct gattattcag ggaatgaaca ccggaatttt gctttctggc
    42541 tttccgccgg agatgaacca ggtggtgaaa gcggtggtgg tgctttgcgt gctgattgtt
    42601 cagtcgcaac gctttatcag tctgattaaa gggggacgta accgtgataa aacgtaattt
    42661 gccactgatg atcaccatcg gcgtctttgt gttgggttat atctactgtc tgacccagtt
    42721 tcccggtttt gcttccacaa gagtgatctg caatatcctg accgataatg cctttcttgg
    42781 gataatcgcc gttggcatga cctttgtgat cctctccggt gggatcgatc tctccgtcgg
    42841 ttcggtgatc gcctttaccg gcgtgtttct ggcaaaagtg attggcgatt tcggcctctc
    42901 gccgctgctg gcgtttccgc tggtgttggt gatgggctgt gccttcggcg catttatggg
    42961 gcttctgatt gacgccctga agatcccggc gtttatcatt acccttgcgg gaatgttctt
    43021 tttgcgtggc gtcagctatc tcgtttcgga agagtcgatc ccgataaacc atcctgttta
    43081 tgacacgctc tcaagccttg cgtggaaaat ccctggcggt ggtcgcttaa gtgcgatggg
    43141 actactgatg ctggcggtgg tggttatcgg catattactc gcgcatcgta cccgttttgg
    43201 taatcaggta tacgccattg gcggcaacgc aacgtcggca aacctgatgg ggatttccac
    43261 tcgcagcacc actattcgca tttatatgct ctccaccgga ctggcaacgc tggcggggat
    43321 tgtcttctcg atttataccc aggccggata tgcactggcg ggcgtaggtg tggaactgga
    43381 tgctatcgcc tcagtggtaa ttggcggtac gcttttgagt ggtggcgttg gaacggtatt
    43441 agggacgctt tttggcgtgg cgattcaggg actgattcag acttacataa actttgatgg
    43501 cacgctgagt tcctggtgga cgaaaatcgc catcggtatt ttgttgttta tttttatagc
    43561 attacagcgt ggattaacgg tgctgtggga gaatcgtcag agttcgccag tgacaagagt
    43621 caacattgcg cagcgataaa aacgcctctc cgtgtggaga ggcgcaggag cttacgcgtc
    43681 cgggaactca cggataaagc gttcgacatc ttcaaccata tggtcgttgc cgacaaagaa
    43741 cgaacggcgc tggtgcaggg tttccgggat aatatccaga atacgctctt tgccatcgct
    43801 cgctttaccg cccgcttgtt ccgccaggaa tgccatcggg ttgcactcat acagtaaacg
    43861 tagtttaccg tccgggtggc tggcggtgct tgggtagaga taaataccgc ctttcagcag
    43921 gttacggtgg aaatccgcga ccagtgaacc gatataacgt gaggtataag ggcggttggt
    43981 ggacttatct tcttcctggc agaatttaat gtacttcttc accccgttcg gaaacttaat
    44041 gtagtttcct tcgttgatgg agtaggtttt gcctttctcc gggaagcgca tccgttcctg
    44101 gcacaggcag aaaacaccca gcgaaggatc ataagtaaag gcgtgaacac cgcatccggt
    44161 ggtgtaaacc agcatagtag aggagccgta taccacgtaa cctgccgcaa cctgtttgtt
    44221 accaggctgg aggaaatctt cttccgttac cggcgtgcca acaggcgtaa cgcggcggta
    44281 gatagagaaa atggtaccga cagagacgtt aacatcgatg ttggacgagc catccagtgg
    44341 gtccatcagc accacgtatt ttgcgtgttc acagccttca aagacgacaa tctcatcttc
    44401 ttcttcagag gcaatgcccg caacgatatc gcgtgctttc agtgcggctt tcagtttctc
    44461 attagcgaac aggtcgagtt tctgctgaac ctcgccctgc acgttctcag caccgctggc
    44521 acccaggata tcaaccagtc ctgctttgtt gatatcgcga tggataatct tggcgcccag
    44581 ttttattgcc gacagcaaag cagtgagctc accggtagca tgagaaaact cgtgctgctt
    44641 ttcgacaata aattcaccta acgttttcat aaaactttcc ctgcaatgtt tatggagtaa
    44701 agcgaccgca acaatcttaa caaataatcc caatgttgcg ctcaggtgaa tcgcgccagc
    44761 aaattacgga ttatcctgaa atgcgtttct cacttgcccg acatatgcgt aaaatgagcg
    44821 ccagattaaa aaaggatagt gacgtatgcg cattcatatt ttaggaattt gtggcacgtt
    44881 tatgggcggt ctggcgatgc tggcgcgcca gttaggccat gaagtaacgg gttcggacgc
    44941 caatgtgtat ccgccgatga gcaccttact tgagaagcaa ggcattgaac tgattcaggg
    45001 ttacgatgcc agccagctcg atccacagcc ggatctggtg attattggta acgccatgac
    45061 ccgtggaaat ccgtgtgtgg aagcggtact ggaaaaaaac atcccttata tgtcaggtcc
    45121 gcagtggctg cacgattttg tgctgcgcga ccgctgggtg ctggccgttg ccggtacaca
    45181 cggcaaaacc accacggcgg gaatggcgac ctggattctg gaacaatgcg gttacaaacc
    45241 gggatttgtg atcggcggtg tgccggggaa ctttgaggtt tcggcgcgtc tgggcgaaag
    45301 cgacttcttt gttatcgaag cggatgagta cgactgcgcc ttcttcgaca aacgctctaa
    45361 atttgtccat tactgcccgc gtacgctgat cctcaacaac cttgagttcg atcacgccga
    45421 tatctttgac gacctgaaag cgatccagaa acagttccac catctggtgc gtatcgttcc
    45481 ggggcagggg cgtattatct ggccggaaaa cgatatcaac ctgaaacaga ccatggcgat
    45541 gggctgttgg agcgagcagg agctggtggg cgagcaaggt cactggcagg cgaaaaagct
    45601 gaccactgac gcttctgaat gggaagtctt gctggatggc gaaaaagtgg gcgaagtgaa
    45661 atggtcgctg gtaggcgaac ataatatgca caatggcctg atggcgattg cggcggctcg
    45721 ccatgttggt gtagcgccgg cagatgccgc taacgcgctg ggttcgttta ttaacgctcg
    45781 tcgccgtctg gagttgcgtg gtgaagcgaa tggcgtcacg gtatatgacg attttgccca
    45841 tcacccgacg gcgattctgg caacgcttgc ggcgctgcgt ggcaaagttg gtggtacggc
    45901 gcgcattatt gccgtgctgg aaccgcgctc aaacaccatg aaaatgggga tctgcaaaga
    45961 cgatctggca ccttcattag gtcgtgccga tgaagtcttc ctgctgcaac cggcgcatat
    46021 tccgtggcag gtggcagaag tggcagaagc ctgcgttcag cctgcacact ggagcggcga
    46081 tgtggatacg ctggcagata tggtggtgaa aaccgctcag ccgggcgatc acattctggt
    46141 gatgagtaac ggcggttttg gtgggatcca tcagaaactg ctggatgggt tagcgaagaa
    46201 agcggaagcc gcgcagtaat tcacagcctc agcctgatag agtgtgtgta ggccggataa
    46261 ggcgttcacg ccgcatccgg catttgagca tagtgcctga tgcgacgctt gatgcgtctt
    46321 atcaggccta caggtgcacc gtatccggca aaccatactc acatcaacaa cgaaaattac
    46381 ccttcgttct ccgctaactc gcgcagatac tggaaaatct ggcgtgcgga tttcggcggc
    46441 ttattccctt ctttctcttt cttcgcgttg cggatcagag ttcgcagttg ctgacgatcg
    46501 gcatcgggcc acagattcaa tacttcagcg atggcgtcat caccctggtc gatcagacga
    46561 tcgcgcaggt tttccagttt atggaacagc accacctgct ggttgtgacg gtttttcagc
    46621 ttatccagcg cctgacggat cggctcaaca tcgcgctggc gcagcatctt accgatgagc
    46681 tgcaactggc ggcggcgacc ttccatctta atacgctggg cgagctcaat agccgcgcgc
    46741 agatccgcat ctaacgggat cttatccagc gcgtttttcc ccagatcaac aatttctgcg
    46801 cccaggcgtt ttagctcctc ggcatcgcgt ttaatttcac ttttactgac ccagataatt
    46861 tcatcgtctt cgtcttcgat gtcatcaccg ggaacgtcgt cgagccagtc ttcgggctgc
    46921 ttagtcatct caggctcctt aaaaaaagag gctaatgtta ccagttaaga tgcgcactga
    46981 aaaacggttc tctgttagac ttcagagaaa ctctctacat tatggcactt gcaatgaaag
    47041 taatctctca agttgaagcg cagcgcaaga ttctggaaga agcagtttcg actgcgctgg
    47101 agttggcctc aggcaaatcg gacggtgcag aagttgccgt cagcaagacc accggcatta
    47161 gcgtaagcac gcgttatggt gaagtggaga atgttgaatt caatagcgat ggcgcgctgg
    47221 ggatcaccgt ttatcaccag aaccgtaaag gtagcgcatc gtctaccgat ttaagcccgc
    47281 aggccattgc ccgcaccgta caggcggcgc tggatattgc ccgttacact tcgccagatc
    47341 cgtatgcagg cgtggcagac aaagaactgc tggcctttga cgcaccggat ctcgacttgt
    47401 tccaccctgc ggacgtttct ccggatgaag ccattgaact ggcggcccgt gctgaacagg
    47461 cggcattaca ggcggataaa cgcattacca acaccgaagg cggtagcttt aacagccact
    47521 acggtgtcaa agtttttggc aacagccacg gcatgttgca gggttactgc tcaacgcgtc
    47581 attcgctctc cagttgtgta atcgccgaag aaaatggcga tatggagcgt gattacgcct
    47641 acaccattgg gcgtgcgatg agcgatctgc aaacgccaga gtgggtcggg gccgactgtg
    47701 ctcgccgtac cttatcgcgc ctgtcaccgc gtaaactttc gaccatgaaa gcgccggtga
    47761 tttttgccaa tgaagtggca acagggttgt ttggtcattt ggtgggggcg atagcgggtg
    47821 gtgcggtcta ccgtaaatcg accttcttgc tggattcgct gggtaaacaa attctgccgg
    47881 actggctgac tattgaagaa catccgcatc tgctgaaagg gctggcatct acaccattcg
    47941 acagcgaagg tgtgcgcacc gagcgccgcg atatcgtcaa agatggcatc ctgactcagt
    48001 ggctgctgac cagctattcg gcgcgcaaat tggggctgaa aagcaccgga catgcgggcg
    48061 gtattcacaa ctggcgcatt gccggacaag gtctaagctt cgagcagatg ctcaaagaga
    48121 tgggcactgg gctggtggtg acggaattga tgggccaggg cgtgagtgcc attaccggtg
    48181 attattcccg tggtgcagcg ggcttctggg tagagaatgg cgagattcag tatccggtga
    48241 gcgaaatcac catcgctggt aatttaaaag atatgtggcg caatattgtc accgtcggta
    48301 acgatattga aacacgcagt aatatacagt gtggttctgt gctgttgccg gagatgaaaa
    48361 tcgccgggca gtaaaaaact ggcgcgacct gccgcgccaa atcttcataa taaaaaagga
    48421 agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt ctcctcattg gtattcagtt
    48481 cggcgtcgtt tgccgctgat ctggaagaca atatggaaac cctcaacgac aatttaaaag
    48541 tggtcgaaaa agccgataac gcggtgcaag tcaaagacgc gttaactaaa atgcgcgctg
    48601 cggcgctgga tgcgcaaaaa gcgacgccgc cgaagcttga aggcaaatca ccggacagcc
    48661 cggaaatgaa agacttccgc cacggtttcg acattctggt cggtcagatt gacgacgcgc
    48721 tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc tgccgcagag caactgaaaa
    48781 cgacccgcaa cgcctatcac cagaagtatc gttaattctt tatttccctg ttgcctgcac
    48841 tcaggtaaca gggaacttat catcgcaaat gatgcaccac ctgattgctg ctgccgcgcc
    48901 agatcaggga agggtctttt aaatcctgca caaatttgcc gtcgaccagc acgttaatca
    48961 aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag tttatagcct gtccacaccc
    49021 agatgtcttt acccggacac tcggcgcgga tgcgttgtac cagtttcaga atatccggca
    49081 cgttttgcgg atgtagcgga tcgccgccgg agagggaaat accctggcgt ttgatacgag
    49141 tgtcattcag atcgttaatg atctggtctt ccattgcttt ggtaaatggc tgaccggaat
    49201 ttacccgcca ggtgcttttg ttatagcaac cggggcattc atgaacacac ccggagacaa
    49261 acagggtgca gcgagtgccg gggccgttga cgatgtcgac aggatagtac tgatgatagt
    49321 tcattatttc acctggctta tcagtccgcg tgtaggctgc atccggttat caacgcctga
    49381 tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc aaatgtaggc cggataaggc
    49441 gtttacgccg catccggcat ttgcttaaca cagagtgaag attaacctat ctgcccattt
    49501 cccaaatgtt taacgcggcg cttaacttct tcctgcttac cagcgttaaa cggacgtgca
    49561 tccgggctac ctaaatatcc gcacacgcgg cgagtcaccg acacacggga ggtgtcatgg
    49621 ttaccacatt tcggacaagt gaagcctttg ctggtgcact caaattcgcc ggtaaagcca
    49681 cactcgtagc attcatcgat cggtgtgttg gtgccgtaat acggtacatg ctgatagctg
    49741 taatcccaga catcttccag cgccttcagg ttgtgctgaa tgtttggata ctcgccgtag
    49801 caaatgaaac caccgttcgc cagcggcggg taaggcgctt caaagtcgat cttgtcatac
    49861 gggttcacct ttttctccac atcaaggtgg aaactgttgg tgtagtaacc tttatcggtc
    49921 acgcccggca ccacgccaaa ctcagcggta tcgagacggc agaagcggtc gcacaggttt
    49981 tcacttggcg tgctgtagag actgaaacca taacccgttt cttctttcca ctgatccacc
    50041 gcctgacgca gacgctcaac aatcgcaata cctttcgcgc gcagctgctc gttgtcgtaa
    50101 acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga tgccgatgta acccagcgaa
    50161 atagacgcac gaccgttttt gaagatttca gaaacgtcgt cgtcagcatt cagacgcacg
    50221 ccacaagcac cttccatata gaggatcggg gccacgcgcg ctttcacgcc ttcgagacga
    50281 gcgatacggg tcatcaacgc cttacgtgcc agcaccagac gttcatccag cagcttccag
    50341 aaggtggctt catcgccttt tgcctccaga gcaatacgcg gcaggttcag gctgatcacg
    50401 ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt cattttccca cacgccgagg
    50461 aagctgcggc agcccatcgg ggttttaaac gaaccggtga ctttcactac ctgatcgtag
    50521 ttcaggatat ccggatacat gcgcttgctt gcgcactcca gcgccagctg tttgatgtcg
    50581 tagttcggat cgcctttttt atggttcagg ccatcgcgaa tcgcaaacac cagtttcggg
    50641 aacaccgcag ttttacggtt tttaccgagg cctgcgatac ggttacgcag gatagattcc
    50701 tgaatcaggc gcgactccca gctggtgccc agaccaaaac caaaggtcac aaacggtgtt
    50761 tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca gcgactggaa ggcatcgtag
    50821 cactctttga tggtacgaga gttagcgtag ccttcggcgt ccgggatgtt ccactcttct
    50881 gcggtcttgc gatgtttgtt gtagctggcg gtgacaaacg gtgccagcac ttcatcgata
    50941 cggttaatgg tggtgccgcc ataaatatgg ctggcgacct gagcaataat ctgcgcagtt
    51001 acagcggttg ccgtagagat cgacttcggc ggttcaatct cggcgttccc cattttaaag
    51061 ccctgggtca gcatgccttt caggtcgatc aacatgcagt tgaacatcgg gaagaacggc
    51121 gagtaatcga gatcgtgata gtgaatatca ccacgttcat gtgcctgcac cacgtcacgc
    51181 ggcagcaggt gctggcgtgc atagtgttta gccacgatcc cggccagcag gtcgcgctgg
    51241 gttggaatca ccttgctgtc tttgttggcg ttttcgttga gcaacgaggc gttggtctgc
    51301 tcaaccagac cacggatctc ctgattcagg cgaccgcgtt tttcacgttc aatgtcgcga
    51361 tcgtgacggt actcgatgta agcacgagcc agttgtttgt atggacccga cataagctgg
    51421 ttttcaacgg cggtctggat ctcgttgata tccacctggc tgcggccctg catctgctcg
    51481 ctgacaaccg cggcaacagt ggcgcaataa tctgcatcat cgacttccgc tgctttagct
    51541 gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg gcactttgca gccgtctcgt
    51601 ttcatcacat gcggtgtcat gatcgctcca tatttttaag aacaggttat ccacagaaat
    51661 tgggaaaggc gttttccggt tttttcgttt cgttttccgg tgctttccgc aatcgccatc
    51721 cgcttatcca caacgccgca cctttttcgt ggggagctgt agtagcaatt atagtcgatt
    51781 aatacaacat attgggttgg gacgcatttt aaagtctata tatagtgctt tgcatcaagg
    51841 atgtttgaac tttttttgat gtagctcaaa gtaaaaagca gagcgtacgg atgacgggcg
    51901 ctacagcgat atgtaaattt tttaatgaat tttttgatta taaaatcaac aaaaacaaca
    51961 tactgacagg caaaaccccg gaatgacagg cattaccggg gcttagcgaa gatttacttc
    52021 tgtaaccacc agacagcctc aaaaggccgt aaagtcatgg tacagggttg tggtgaggct
    52081 tcttcgtagt tatgcatcac aagctgccag ttgccgagca ttttccctgg ctgccagggt
    52141 tgggtctcac ggctaaggtt ggcaatgacc agcaaggttt gccccttcca ctcacggcga
    52201 tagcaccaca atacagggct gtttggcagc agatcctggt aatcgcccca tgtcaggacg
    52261 gcttcttgct tacgcagtgc gattaacttt tggtaggtgt aaaacaccga ggactcatcg
    52321 gccagcgctg cttctacgtt gatttcctga tagttatcgc ccaggccaat ccacggttcg
    52381 ccagccgtaa acccggcatt atcgccgttg gtccattgca tgggtgtgcg actgttgtca
    52441 cgggatttac tggcgaggat tgtcaataac tcgtcggcat cacgcccatc gttgcgcagc
    52501 tcggcaaaca tattgaggct ctccacgtcg cgatagtcag taatgcgcgt gaaatgtggg
    52561 ttggtcatgc caatctcttc gccctggtag atatacggcg ttccctgcat gccatgcagc
    52621 accatcgcca gcatttttgc cgcaggcacg cggtattcac cttcatcacc aaagcgagaa
    52681 acaatgcgcg gctgatcgtg gttacaccag aacaaggcat tccatgccac gttgtgcatt
    52741 ccttgttgcc agtggcggaa caatgttttc aacgccacaa agtcaggttt agccagcgtc
    52801 catttttcac caccgggata atcgaccttc aggtgatgaa aattaaaggt catcgacaat
    52861 tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa ggctggtgga ggacatttca
    52921 cctacggtca ttaacccgcg tggcgtaaac acatcgcggt tcatctcgtg caaaaactcg
    52981 tgtgctcgtg gcccgtcggt atagaagcga cgcccatcgc catccaggtc ttcagggtaa
    53041 cgcgggtctt tggagatcag attcaccaca tccaggcgca acccgtcgac cccacgatcg
    53101 gcccagaact cacagacttt tttcagctct gcgcgtaccg ctggattctc ccagttgaga
    53161 tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt cgctttctgc atgccagcgc
    53221 cacgcactac caccgaattt tgaacgccag ttgttcggtg gcgtttctgg ttctccatcg
    53281 cgccagatat aaaactggcg gtaagggctt tctttgttca gcgcctcgcg aaaccaggca
    53341 tgttgggtag aggtatggtt aaacaccata tcgagaatga tacgaatccc acgcgatttt
    53401 gcctgtgtca ccagttcgtc aaaatcgtcc agcgtgccat aggtgggatc aatcgccgta
    53461 taattcgcta cgtcgtaacc gttatcgacc tggggagaga cataaaaggg ggttagccag
    53521 atggcatcaa cgcccagttt atgcagatag tccaggcgtt ggataacgcc acgtaaatcg
    53581 ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat aaatctggta gataacgccg
    53641 ttttgccacc agtggggaag atgagtcatt acgttattcc tgcaaatgcg agggggcgca
    53701 attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc aggcggtatt tccgctgata
    53761 gataaacgag gtgagtacaa tcgggatgat gatggcgata gccattgcca gcgcaaacac
    53821 ctgccagtag ctcggttgaa tcgagagaat tcccggcagg ccgcctacgc cgatgccatt
    53881 cgccataacg ccgttcaggc cgcatagcaa tcctgccaga ccagaaccaa tcatcgcgca
    53941 cagcatcggg aagcgatatt tcaggttgat gccgtacata gcaggctcag tgactccaag
    54001 ccaggcggag atagcggcag gcacggagat ctcgcgttca ttgtgcttgc gactggaaat
    54061 gataatacct atcacggcgg agccctgagc aatattcgac agcgcaatca gcggccacac
    54121 tggcgtgcca cccatgcttt gaatcatctg caaatcaata gcaagcgtgg tctggtgtac
    54181 gccggtgatc accagcgggg cgtacaggaa gccaaacaat gccgcgccaa tcggagcaaa
    54241 gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca tcgccaatca tgcgaccaaa
    54301 cggaccaatc agcgcatggg cgaggaacac cgcgaggatc agcgaacaga cgggcaccac
    54361 caccagatag aggtaatccg gcacgatgcg cttaagacga gtttcaataa cgcccagcgc
    54421 cagtccggct aacagtgccg ggatgacctg cgcctgatag cccactttgg cgatgctgaa
    54481 catgccaaag tcccatactt ccggcagctg ctgcccgagc agataagcgt tcatcagctg
    54541 tggagaaacc agcgttacgc caagcacgat accgaggatc ggtgtaccgc ccattttttt
    54601 caccgctgac cagcaaatcc cgaccggcag gtagaagaag atcgcttcac cgatcaacca
    54661 cagaaaatcg tagatcgttt gcagggaagg gtacatttgc gccagcgtct gaccgttgct
    54721 catgggcaaa tcgccgatca cattgcgaaa accgaggatc aaaccaccac taatcaacgc
    54781 gggcagcaac gggaagaaga tctccgcgaa atgagagatc aactgctcat gccatttcat
    54841 attctgccgg gcggcttttt ttacctgctc tttatcaacc tgcgcctgtc cggttgacgc
    54901 aatcagtgct tgatagtaat cacccacgtt ggtgccaatc accacctgaa attgcccggc
    54961 attggtgaaa cagcctttca ccatggggag ttgctcaatt tctttcggtc tggcattgac
    55021 cggttggttg aggacaaagc gcaggcgagt aatacagtgg ctcaccgtcg caatattgcc
    55081 gcgcccgccg accagttcaa tcaaccgatc gatatccgtt tggtttattt tgctcatcat
    55141 aaagccccat ggcagatgac atttttggtt ggctgcagaa tatattgcgc ggaaaaattt
    55201 aaaaacggga acgttcccga aacgcagcga agatcacaat ttatcgttca ggaaacgatc
    55261 aggacagggt ggcggggatg atgatttgtt gcggttcgct gcgcccggtt acctgcgcga
    55321 tcaactggca ggccgcctgg cgtccagctt cggcataacc gggatctacg gtgacgatct
    55381 ccggatggag gaatttcatt aacggtgtat taccgacgct cgccagttgc aaggtgtcga
    55441 tgcgttgctc ttgcaggtat ttacttgcgc caagtgccag cgtgtcggtt gcgcacagta
    55501 atgcggtagt ttcaggcgta atcacttttg ctacgttctc atagccttgc ttcatagcaa
    55561 gccctggcag agcggcaacg ggatgcaatt tatgcgtttt gcagaacgcc aggtaggctt
    55621 cgtgacgtcg cttaccggtt gtcacgtcac tgtgcggcac gccgagatag ctgatattac
    55681 gatgtccctg gtcatacagc cgttgcatca ggatcttgat tgccccttcg tcgtcataac
    55741 agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac cagcgatgac tgccagtggg
    55801 ctaacatttc ttctgttatg ccggtaaaac cgaacagcac tacgccgtcg atattacgcc
    55861 gtttcagcac ccccaaatgt tcggcaacta attgtgggga aaactgactt tccatcatga
    55921 ttggatcgta accttgttca tagaacgctg gcagcatggt ttgaacggcg agattttctg
    55981 acaacgaatc cagacgggta acaatgatgg cgaccacttt atcgctttgc ccacgcatag
    56041 cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat cactgcttca acacgctcgc
    56101 gcgtgcgctg gctcacgccg ctttcgttat tcagcacccg ggaaaccgta gatttcccca
    56161 cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt ttgcatcctg ttgtcccata
    56221 acgtgttgtt taattatttg agcctaactt tacctgtgca ttcagcaatg ggtaaagtct
    56281 ggtttatcgt tggtttagtt gccagcaggt attatatcgc cataaatgct acgaatatta
    56341 ttggattctc cttattattt gcggcgcttt tttcacttac cggaggttat atggaacctg
    56401 atcccacgcc tctccctcga cggagattaa aacttttccg gtaagcccgt cttttcacgg
    56461 cgttaccgga tgcgtaaggc cgtgacgttt taacgtccct gctcagcttt attaccttca
    56521 ggtaaggctt cgccacgcct gaagacattt ctgtactgtt tcagacagtg cggagggact
    56581 ccttatgttt aaagaaattt ttacccggct cattcgccat ttaccttccc gtctggttca
    56641 tcgtgatcca ttgcctggcg cgcagcagac agtgaatgcg gcggtcccgc cttctttaag
    56701 tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa ttgtggaaaa cgttcgacac
    56761 ccatccggaa gggttaaatc tggcggaagt ggaatctgcc cgcgaacaac atggtgaaaa
    56821 taaattaccc gcacaacaac cgtcgccgtg gtgggtacat ttatgggtct gctatcgcaa
    56881 cccctttaat attttgctca ccattcttgg tgctatttct tacgccacgg aagatttatt
    56941 tgccgcaggt gttatcgcgc taatggtcgc tatttctacg ttgctgaact ttattcagga
    57001 agctcgttcc accaaagcgg cagatgccct gaaagcgatg gtcagcaata ctgcgacggt
    57061 gctgcgcgta attaacgaca aaggcgaaaa tggctggctg gagatcccga tcgaccagct
    57121 ggtgcccggc gatattataa aactggcggc gggggatatg atcccggcag atttacgtat
    57181 cttgcaggcg cgggatctgt ttgtcgctca ggcgtcgtta accggtgagt ctctgcccgt
    57241 agaaaaagcc gctaccactc gccagccgga gcacagcaat tcgctggagt gcgacaccct
    57301 gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa gcaatggtga ttgccacagg
    57361 tgccaacacc tggtttggtc aactggcggg gcgtgttagt gagcaggaaa gcgagccgaa
    57421 tgcctttcag caagggatca gccgcgtcag tatgctgctg attcgcttta tgctggtgat
    57481 ggctccggtg gtgttgctta tcaacggtta caccaaaggc gactggtggg aagcggcact
    57541 gtttgcgctt tcagtcgcgg taggcttaac gccagaaatg ttgccgatga ttgttacctc
    57601 gacgctggcg cgtggcgcag tcaagttgtc gaaacagaaa gtgatcgtca aacatctgga
    57661 tgccattcag aactttggcg caatggatat tctgtgcact gataaaaccg gcaccctgac
    57721 acaggataaa attgtgctgg agaatcatac cgatatctcc ggtaaaacta gcgaacgcgt
    57781 gctgcatagc gcgtggttga acagccatta ccagaccggg cttaaaaacc tgcttgatac
    57841 agcggtgctc gaaggtacgg atgaagagtc agcgcgctcg ctggccagcc gttggcaaaa
    57901 aattgatgag attccgtttg atttcgagcg tcgtcggatg tcggtggttg tggcagaaaa
    57961 taccgaacac catcagttgg tttgcaaagg cgcattgcag gaaatcctca atgtgtgttc
    58021 gcaagtgcgt cacaatggcg agattgtacc gctcgatgac accatgctgc gtaagattaa
    58081 gcgggttact gatacgctga atcgtcaggg gctgcgcgtg gttgcggtgg cgacgaaata
    58141 cctgccagcg cgtgaaggag attaccagcg ggcggatgaa tccgacctga tcctcgaagg
    58201 atatattgct ttccttgatc cgccaaaaga gacaacagct ccggcactga aggcattaaa
    58261 agcgagtgga attaccgtaa aaatccttac tggcgacagt gagttagttg cagcgaaagt
    58321 gtgccatgaa gtggagctgg acgcgggaga ggtggtcatt ggtagtgata ttgaaacgct
    58381 atctgacgac gaactggcaa atctcgcaca gcgcaccacg ctgtttgctc gcctgacgcc
    58441 gatgcataaa gaacgcattg tgaccttact gaagcgcgaa gggcatgtgg ttggctttat
    58501 gggcgatggt attaatgatg cgcccgcctt acgtgctgcg gatatcggca tttctgtgga
    58561 cggcgcggta gatattgccc gtgaagcggc tgatatcatc ctgctggaaa aaagcttgat
    58621 ggtgctggaa gagggggtta ttgagggacg tcgcactttc gccaacatgc tgaaatacat
    58681 caaaatgacg gcgagctcta acttcggtaa tgtgttcagc gtgctggtgg cgagtgcttt
    58741 cttgcccttc ctgccgatgt tgccgctaca cttgcttatt caaaatctgc tgtacgatgt
    58801 gtcacaggtg gcgatcccat ttgataacgt cgacgacgag caaattcaaa agccgcagcg
    58861 ttggaatccg gcggatctgg ggcgctttat ggtcttcttc ggaccgatca gctcgatttt
    58921 cgatattctg acgttttgcc tgatgtggtg ggtattccat gccaacacgc cggaaacgca
    58981 aacgcttttc cagtcgggct ggtttgtggt gggcttactg tcgcaaacgc tgattgtgca
    59041 tatgatccgc acccgtcgtg taccgtttat tcagagctgt gcatcgtggc cgttaatgat
    59101 catgaccgtg atcgtgatga ttgtcgggat cgcattgcct ttttcaccgc tggccagtta
    59161 tctgcaactg caggcgctgc cgttaagcta tttcccgtgg ctggttgcga ttctggcagg
    59221 gtatatgacg ttaacccagt tggtgaaagg gttctatagc cgtcgttacg gttggcagta
    59281 aagaataaac tgggcatggt ggcccggaat cagattgcag acaaagtgag ctttgttcat
    59341 gccggatgcg gcgtaaatgc cttatcaggc ctacaaattc aataaattgc aggaatcacg
    59401 taggcctgat aagcgtagcg catcaggcaa ttttgcgttt gtcattagac ttaatccggg
    59461 catgatagcc cggatttcca tcaagattag cgacgaacag cgatcgcttc gatctcaatc
    59521 ttcacgtctt tcggcagacg ggcaacttca acgcaagaac gtgccgggaa ggtggcgttg
    59581 tgttcggtga agaaggcttc gtaggtagca ttaacggttg cgaagtcgtt cagatctttc
    59641 acaaacacgg tggttttaac gatatcgccc actttcaggc cagcggcttc gacgatcgct
    59701 tttacgttat ccagcgactg acgcgcctgt gcagcgacat ctgccgggac ttcgcccgtt
    59761 ttcgggttta ccgggatctg accggaagtg ataatcatat tgcccagatc aacgccctgt
    59821 acgtaaggac cgatagctgc cggtgcattt tccgtcgcga tagttttgct catgatttct
    59881 cctttattac agcggtaaaa agtctggctc attataggga gccagacttt tattaccaac
    59941 cgcaattaat tggccagcac cacattatga gaaaactctt tttcacagta tttgcatttg
    60001 agcgcgatat cattggcgcg ttttcgcacg gcaaagctgg atgaaaccgg ttcggcatgg
    60061 ctgatacagt tgctgttcgg gcagaccagc acattgtcga tgcgctctgg cagactgggg
    60121 cgcgatttgc caaccacttc atagttgtcg atacggttaa ccgtggcttg cggtgcatat
    60181 aacgccagct gatcaacctg atcctcactt aaaaaggtat tttcgatttt gatcagatcc
    60241 ttgcgcccca tctcgccaga aggcaggttc agaccaatgg tgatgcgctg atctgtttcg
    60301 gtcaacttga acagactcaa cagtttaaaa ccgatctggg cggggatatg gtcaattacc
    60361 gtgccgcgtt taatagcttc aacctgcaat ttattatcgt gtgtcatctc tatttcccct
    60421 tacagtacca gatcgcgatt cagaaccagt gccagtaacg cctggcgagc gaaaatcccg
    60481 ttgcctgctt gctggaagta ccaggcgtga ggcgttttat ccacatccgt cgcaatctca
    60541 tcaacacgcg gcagcggatg cagcactttc atattggctt tggcgttgtg gagatcgctg
    60601 gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg acgggtccag acgctctttt
    60661 tgcacgcggg tcatgtacag gatgtctact tccgccatca cttcttcaat agagctgtgc
    60721 agactccagg cgatcccttt ttcatcgagc atatccagaa tgtattgcgg cattgccagc
    60781 gcatccggcg cgataaagta aaaacggttg ccgtcgaact tcgctagcgc ctgggtcagg
    60841 gagtgaacgg tgcggccata tttcaggtca ccaaccattg cgacgtggag attgtccaga
    60901 cgcccctggg tttcctgaat ggtgaataag tccagcaagg tttgcgtcgg atgttggttg
    60961 gagccatcac cggcattcag taccggtaca ttgccggaaa actcggtggc caggcgcgct
    61021 gcaccttcct gcggatgacg catcactatc gcatcgacgt aagtgctgat aaccgaaatg
    61081 gtatcggcca gcgtttcgcc ctttttaccc agtgatgtat tggcgctgtc ggagaagccc
    61141 accacgctgg cacccaggcg gtgcatggaa gtttcgaaag agaggcgggt gcgggtagag
    61201 gcttcgaaga aacagctggc aataactttg tgcttcaaca gctctggttg cgggttcgct
    61261 ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat cgcgactaag gtcgtttatg
    61321 gaaatgatat gtttctgata tagcggatta gccatctttt atctcctgac gcctgggcaa
    61381 aaaaaagccc ctcgattgag gggctgggaa tgggtgatca acgggaagaa aaacggcagg
    61441 ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa cacactgaac catacatcct
    61501 cccggcaaat tgtccggcat tatactcagc tctgagagag gatcaagaag atgatgcagt
    61561 tataaagcaa ggcaaacggt tatcatgaat atatattcaa ttttaataag taattaattt
    61621 gcttgtacac cagggctgtg cgctttacca cacgtggtgc gacccaaacg atattgctgc
    61681 cagcaatcac gccaagaatt tctggcaaag catggaaatc aaggattcgt gctactgccc
    61741 gaccatatcc ggcgacggta tggataagga taaattcacc attatgctcg acactcagga
    61801 ccatttcagc gacggatcga cctgcgtcag gtgtgggaag taattgagga ttcaccgaat
    61861 aaattttttg tccttttgta tttctaattt ttatgactcc cagcaatttc agtagtcgtg
    61921 aaacggttga ctgactgata ctgtcaaagc catggttttg caaatcgcgg cggatttcct
    61981 cctgtgaaag ataacttttt tcggtaatca ggcgctgaca gaccgccagt tgcttctttt
    62041 ctttggcgga gtaatcatcg tattccttca taatgcacct tttatttatt attacctgat
    62101 gatatttttc acattttata atgtgagagt ggttcattta tcactacacc agatttgaag
    62161 gccgctttga taagcggcct gtcgcgttaa atcatcgcgg cgatactcag cacaatcata
    62221 ttcaacatcg tgagaatcag cagtagcggt gctacccatc tcagataacg gacccagggt
    62281 acacgggcaa tcgccagacc gcccatcact accgcagagg ttggcgtaat caaattaacc
    62341 accccggaag cagactgata agcggtcacc accagatcgc gttgtacatg agcaaaatca
    62401 gcgagtggag ccattattgg catcgttaac actgcaaggc cggaagatga gggaaccaga
    62461 aaagagagca atacttccag ccaataggtc acgttgatga aaacagtggt ggaaagccct
    62521 gaaaccagat tctcagcact gtgcagaatt gtgtgcgtaa tcatgccgtt atccatcacg
    62581 accacaatac cgcgggcgat gccgataatc agtgccacac caaggagatc ccgcgcgccg
    62641 tcaataaagg tactggtgaa ggtttcttcg cccatgcggg caatgacacc cacgatgacc
    62701 gcagctgcca gaaagacagc agagatttcc gccatccacc aacccagtac cgctacgccg
    62761 tagatcataa aggcaaagga tgcagcaaaa agtatcagga tcgctttgcg cgtgggggta
    62821 aattccagca tcgtttctga acgatttccc agaaaatgcg cctggttttc agccatctta
    62881 tccgccacga tagagctttc cggactgttg cgaactttgc gggcataacg cataacccag
    62941 taaacacaaa tcacgtagcc tactaacagc aaaataacac gcagcaaaat gccctgagta
    63001 aaggggatcc ctgcggcgtt ggcagcaatg acggtagcaa atgggttaat ggttgagccc
    63061 agcgtgccaa tgcccgatcc caataacact gtggcggcag cgaccagagg atcaaagcgg
    63121 gcagccatca tgacgggcac cagcaacgtg tagaagggga gtgattcttc cgccatccca
    63181 taaatagtac cgccagcagc gaagagcgcc atcaggatcg gaatcatcca ctcttcccga
    63241 ccattaaggc gagttgtcac tcgttcgata ccggcatcta ttgccccggt tttatttact
    63301 actcccaaaa atccaccgat gatcaggaca aacagcgcga catcaatcgc tcccgcagtg
    63361 taggtttgat ggttgtacaa gccgtcgatg ggcgctaaca ggacagccgt aataccctgt
    63421 ggttgtgcct caacaggtgc gtaagtgcct gcaacaggaa cttcctttcc cagtgcggca
    63481 tttgtcacca tctgatattt tcccgcaggt acaatccagg tcattgccgc aactaacgca
    63541 atcagcacga acaagatggt ataagcggtg ggaaatttga atttacccat aatgtgatct
    63601 ccataatcac gggcatacgc cactcgtcga cgcatgcccg tcagggttag tcgccgagag
    63661 ttgctaccat caccgcttta atggtgtgca tccggttttc tgcttcatcg aaaacaatag
    63721 agtgggccga ttcaaaaacc tcttcagtaa cttccagtcc tttgaggccg taggccatct
    63781 cgatctcgcg tcctactgtg gtgtgctcat tatggaaggc gggcaggcag tgcataaatt
    63841 taacttcagg gttacgcgtg gcagtaatca cctgttgatt gatctgataa ggtgccatca
    63901 gactcactcg ttctgcccag gcttcttttg gctctcccat cgaaacccaa acatccgtat
    63961 agagaaaatc aacgtcgtat acgccttctt ccacatcctc tgtcagggtg attctcgccc
    64021 cagtcacgct ggcgatctca cggcattgtg ttaccagcgc ttcatctggc cagaaagatt
    64081 ttggtgccac cagacggata tccatcccca tttttgcagc accaaccatc agcgagttcc
    64141 ccatgttatt tcgggcatca ccaagatacg caaagctcag ctctggcagc gttttgcctg
    64201 gcgcatgttc cagcatggtc attaaatccg cgaggatctg tgtcgggtga aattcattgg
    64261 tcagtccgtt ccataccgga acaccagcga actcacctaa ttcttcaacg atattctgac
    64321 cataaccgcg atactcaata ccgtcataca tgcgtccgag tacgcgagcg gtatctttca
    64381 ttgattcttt atggccgatt tgtgagccgc tggggccgat gtaagtcacc tgcgcacctt
    64441 gatcaaaggc cgcgacttca aaggcacagc gggtacgggt agaggatttt tcaaaaatca
    64501 acgcaatgtt tttaccgacc agcgtttgtt tttcgtttcc agatttcttg gcggctttaa
    64561 gattgatagc caaatcaatc aggtattgaa tctctgctgg ggtgtaatca agcagcttaa
    64621 gaaaattacg gtttttcagt gaagtagcca tggggatgtc cttataaaaa tggatcaagc
    64681 gtggttatgg gggtatcaag gcgaataaga gtgcctttgt ctccggcgag aatttccgga
    64741 ccatctgcaa gagagccgat ccctgcaatg ccccggcact gactaacaaa ctttgcacag
    64801 gcggtgactt tcggccccat agacccggca tcaaactgca tttcgttgag taattccggc
    64861 gtgacctggg ccagcgggcg ttgggttggc ttgccccagt cgagatatac tgcgtccgca
    64921 tcagtgagga tcaacaaggc gtcagcatgg atctgacttg ccagtagcgc ggctgaaaga
    64981 tctttgtcga taaccgcttc aatcccatgg tatccatcgg ctttttcaac caccggaaca
    65041 ccaccgccac cattacagat caccagatga tcgtgggcga tcagtgtctg gatggcatcc
    65101 cgctcgacga tgcgtttagg ttgtggagaa ggtacgaccc ggcggaaaga gtgaccatct
    65161 gctttgaaaa cccagccttt ttctgcctgt agtacctgag tctgagcgtg gtcataaatt
    65221 gggccgatgt acttagttgg gttactgaag gcgggatcgt tggcatccac ttcaacctgc
    65281 gtcagcagca cgctgatttc acgttgtggt agttgatttt tcagcgcctg ttgtagcatg
    65341 tagccgatca tcccctggct ttcggccccc agaatgtcga gtggataggg agcgacatgg
    65401 gcataggcgc tattttgaag tgccagaagt ccaacttgtg gaccattccc gtgaaccagt
    65461 acaacccgcc agtgttgagt cagttgggcg atagttttag ccgcaagatc gatattttta
    65521 cgttggattt ctgcttcgag tggttcgcct cgtttaagca gggcattacc gccgagagcg
    65581 ataaccagtg ttggtttgtt ttccatgatc tcttccttag ataccgtcac gatgcaaagg
    65641 gcaactcatg caacgtgcgc cgccacgtcc gcggcctaat tcatcaccgg ggatgggtaa
    65701 aacggtgatg cctgctttgt catatttttc gttagtccag atgttgcgct cgtagcccac
    65761 taccactccc ggccgaagcg tgagcacatt gttagcgtca ttccactgtt cgcgttctgc
    65821 ctcaaaggca tcaccccccg tggtgattaa acgtacctgg tcaataccga gggctttttc
    65881 gatagcatgg aggagtgtgc tttcttgagt acgtttcagg ccgccgtgac cgtcaggtgt
    65941 tagcgtccag caattaacat ccgggcgcac aacttcggga taaaccgaga aggtgtcgat
    66001 atcgatatga gtcataacgg tgtcaagatg catacaggag cggtgtttag gcagctcaac
    66061 agcgattacc cgttcagcct gacgatgttt aaacaatgcc tgagcaagaa actcgactcc
    66121 ttgtggcgtg gtgcgttcag acatgccgat caacacggcc ccccgaccaa tcaccagcac
    66181 atcaccacct tctaacgtgg cgtggtcata attaatattc tcgtcgccga aatatttaat
    66241 aaattcgccg ccagcaaatt gtggatgcca gcgataaata gcgcgtagat tattggtttc
    66301 acgttggcga gcaggttttg ccattggatt aatcgataca ccgttataaa tccagcatga
    66361 ggtatcacgc gtaaataaat gattcggtaa cggcttcata atgaaatcat taatatcgtg
    66421 ggtatcaacc accatatttt taattgaggc gggaatttca ctataagtca gcccaccact
    66481 taaatgacga gcaaggtccc ggtgtgacat ctctgcaagc caggtgcgga cgtcagtagc
    66541 aaacgtaggg ccgagtcggt aatctgatat ttgtgtttcc agtaaccagc ttttggcttc
    66601 cgggatatcc aatgtttgtg ttagtaaatc agtgagtaac agaacttcta tcccttgctg
    66661 gcgcagcgta ttggcgaaaa tatcatgctc ttcacctgcc cgctcaacgg acaatacatc
    66721 gtcaaataac aactcctggc aattggatgg tgttagacgt ttcagactga gatttgggcg
    66781 atgcaacata acgctgcgta gttgtccaat ttcagaacca acataatgct tttccatcat
    66841 tattccttta acactttttc aaaaataaag aggagatggc tgcaaacaaa tgaaatgctt
    66901 gtagtcaggt ctgttcagta attttcagat gcaatgattc agtttatttc ttccagaaaa
    66961 tgtgacgact ttcacaccgg cggtgatatt aataatcgaa gttgataata tttgatgcgg
    67021 ttctgataaa tgataaagaa acaaaatacg ataatgcatt aatacgcaaa tatacgcaga
    67081 gttattcttc
//
LOCUS       DADTFG010000005        64997 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.005, whole genome shotgun sequence.
ACCESSION   DADTFG010000005 DADTFG010000000
VERSION     DADTFG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.005"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(64..543)
                     /gene="folK"
                     /locus_tag="J1138_000349"
     CDS             complement(64..543)
                     /gene="folK"
                     /locus_tag="J1138_000349"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBA8452197.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW"
     gene            complement(540..1958)
                     /gene="pcnB"
                     /locus_tag="J1138_000350"
     CDS             complement(540..1958)
                     /gene="pcnB"
                     /locus_tag="J1138_000350"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBA8452198.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1997..2923)
                     /gene="gluQRS"
                     /locus_tag="J1138_000351"
     CDS             complement(1997..2923)
                     /gene="gluQRS"
                     /locus_tag="J1138_000351"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBA8452199.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLYWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2960..3415)
                     /locus_tag="J1138_000352"
     CDS             complement(2960..3415)
                     /locus_tag="J1138_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HBA8452200.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3593..4297)
                     /gene="sfsA"
                     /locus_tag="J1138_000353"
     CDS             complement(3593..4297)
                     /gene="sfsA"
                     /locus_tag="J1138_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBA8452201.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4312..4842)
                     /gene="thpR"
                     /locus_tag="J1138_000354"
     CDS             complement(4312..4842)
                     /gene="thpR"
                     /locus_tag="J1138_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBA8452202.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRGASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4916..7345
                     /gene="hrpB"
                     /locus_tag="J1138_000355"
     CDS             4916..7345
                     /gene="hrpB"
                     /locus_tag="J1138_000355"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBA8452203.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWMDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMATLGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7439..9967
                     /gene="mrcB"
                     /locus_tag="J1138_000356"
     CDS             7439..9967
                     /gene="mrcB"
                     /locus_tag="J1138_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBA8452204.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVMIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10187..12445
                     /gene="fhuA"
                     /locus_tag="J1138_000357"
     CDS             10187..12445
                     /gene="fhuA"
                     /locus_tag="J1138_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBA8452205.1"
                     /translation="MAPSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDQEALKASPCLSIPQSQWGHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSE
                     RDDKEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNLVG
                     SYTYTDAEYTTDTNYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            12496..13293
                     /gene="fhuC"
                     /locus_tag="J1138_000358"
     CDS             12496..13293
                     /gene="fhuC"
                     /locus_tag="J1138_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBA8452206.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13293..14183
                     /gene="fhuD"
                     /locus_tag="J1138_000359"
     CDS             13293..14183
                     /gene="fhuD"
                     /locus_tag="J1138_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBA8452207.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAVYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14180..16162
                     /gene="fhuB"
                     /locus_tag="J1138_000360"
     CDS             14180..16162
                     /gene="fhuB"
                     /locus_tag="J1138_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBA8452208.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16197..17477)
                     /gene="hemL"
                     /locus_tag="J1138_000361"
     CDS             complement(16197..17477)
                     /gene="hemL"
                     /locus_tag="J1138_000361"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBA8452209.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17702..19123
                     /gene="clcA"
                     /locus_tag="J1138_000362"
     CDS             17702..19123
                     /gene="clcA"
                     /locus_tag="J1138_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBA8452210.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASARENT"
     gene            19147..19212
                     /locus_tag="J1138_000363"
     CDS             19147..19212
                     /locus_tag="J1138_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452211.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19205..19549
                     /gene="erpA"
                     /locus_tag="J1138_000364"
     CDS             19205..19549
                     /gene="erpA"
                     /locus_tag="J1138_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBA8452212.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19596..20219)
                     /locus_tag="J1138_000365"
     CDS             complement(19596..20219)
                     /locus_tag="J1138_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBA8452213.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20257..21057)
                     /gene="btuF"
                     /locus_tag="J1138_000366"
     CDS             complement(20257..21057)
                     /gene="btuF"
                     /locus_tag="J1138_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBA8452214.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21050..21748)
                     /gene="mtnN"
                     /locus_tag="J1138_000367"
     CDS             complement(21050..21748)
                     /gene="mtnN"
                     /locus_tag="J1138_000367"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBA8452215.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21832..23349
                     /gene="dgt"
                     /locus_tag="J1138_000368"
     CDS             21832..23349
                     /gene="dgt"
                     /locus_tag="J1138_000368"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBA8452216.1"
                     /translation="MTQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23479..24903
                     /gene="degP"
                     /locus_tag="J1138_000369"
     CDS             23479..24903
                     /gene="degP"
                     /locus_tag="J1138_000369"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBA8452217.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGLGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25081..26211
                     /locus_tag="J1138_000370"
     CDS             25081..26211
                     /locus_tag="J1138_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HBA8452218.1"
                     /translation="MAQDIVARTMRIIDTNINVMDARGRIIGSGDRERIGELHEGALL
                     VLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLAGEPENLRKYGELVCMT
                     AEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDLNQPRVVAIV
                     EVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNSFGRWDAEDH
                     RKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQRMPESRCYF
                     YQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHNVQPLATSKA
                     LFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26264..26650)
                     /locus_tag="J1138_000371"
     CDS             complement(26264..26650)
                     /locus_tag="J1138_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBA8452219.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26969..27793)
                     /gene="dapD"
                     /locus_tag="J1138_000372"
     CDS             complement(26969..27793)
                     /gene="dapD"
                     /locus_tag="J1138_000372"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBA8452220.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27824..30496)
                     /gene="glnD"
                     /locus_tag="J1138_000373"
     CDS             complement(27824..30496)
                     /gene="glnD"
                     /locus_tag="J1138_000373"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBA8452221.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30558..31352)
                     /gene="map"
                     /locus_tag="J1138_000374"
     CDS             complement(30558..31352)
                     /gene="map"
                     /locus_tag="J1138_000374"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBA8452222.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31720..32445
                     /gene="rpsB"
                     /locus_tag="J1138_000375"
     CDS             31720..32445
                     /gene="rpsB"
                     /locus_tag="J1138_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBA8452223.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32580..33431
                     /gene="tsf"
                     /locus_tag="J1138_000376"
     CDS             32580..33431
                     /gene="tsf"
                     /locus_tag="J1138_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBA8452224.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33578..34303
                     /gene="pyrH"
                     /locus_tag="J1138_000377"
     CDS             33578..34303
                     /gene="pyrH"
                     /locus_tag="J1138_000377"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBA8452225.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34453..35010
                     /gene="frr"
                     /locus_tag="J1138_000378"
     CDS             34453..35010
                     /gene="frr"
                     /locus_tag="J1138_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBA8452226.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35102..36298
                     /gene="ispC"
                     /locus_tag="J1138_000379"
     CDS             35102..36298
                     /gene="ispC"
                     /locus_tag="J1138_000379"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBA8452227.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVARMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36487..37245
                     /gene="ispU"
                     /locus_tag="J1138_000380"
                     /gene_synonym="uppS"
     CDS             36487..37245
                     /gene="ispU"
                     /locus_tag="J1138_000380"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBA8452228.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37258..38115
                     /gene="cdsA"
                     /locus_tag="J1138_000381"
     CDS             37258..38115
                     /gene="cdsA"
                     /locus_tag="J1138_000381"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBA8452229.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38127..39479
                     /gene="rseP"
                     /locus_tag="J1138_000382"
     CDS             38127..39479
                     /gene="rseP"
                     /locus_tag="J1138_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBA8452230.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39509..41941
                     /gene="bamA"
                     /locus_tag="J1138_000383"
     CDS             39509..41941
                     /gene="bamA"
                     /locus_tag="J1138_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBA8452231.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42063..42548
                     /gene="skp"
                     /locus_tag="J1138_000384"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42063..42548
                     /gene="skp"
                     /locus_tag="J1138_000384"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBA8452232.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42552..43577
                     /gene="lpxD"
                     /locus_tag="J1138_000385"
     CDS             42552..43577
                     /gene="lpxD"
                     /locus_tag="J1138_000385"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBA8452233.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPKPAQNIAP
                     SAVIDETAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43682..44137
                     /gene="fabZ"
                     /locus_tag="J1138_000386"
     CDS             43682..44137
                     /gene="fabZ"
                     /locus_tag="J1138_000386"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBA8452234.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44141..44929
                     /gene="lpxA"
                     /locus_tag="J1138_000387"
     CDS             44141..44929
                     /gene="lpxA"
                     /locus_tag="J1138_000387"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBA8452235.1"
                     /translation="MIDKSAFVHPTAIVEEGATIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTVHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44929..46077
                     /gene="lpxB"
                     /locus_tag="J1138_000388"
     CDS             44929..46077
                     /gene="lpxB"
                     /locus_tag="J1138_000388"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBA8452236.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46074..46670
                     /gene="rnhB"
                     /locus_tag="J1138_000389"
     CDS             46074..46670
                     /gene="rnhB"
                     /locus_tag="J1138_000389"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBA8452237.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46707..50189
                     /gene="dnaE"
                     /locus_tag="J1138_000390"
     CDS             46707..50189
                     /gene="dnaE"
                     /locus_tag="J1138_000390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBA8452238.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGTAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50202..51161
                     /gene="accA"
                     /locus_tag="J1138_000391"
     CDS             50202..51161
                     /gene="accA"
                     /locus_tag="J1138_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBA8452239.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51259..53400
                     /gene="ldcC"
                     /locus_tag="J1138_000392"
     CDS             51259..53400
                     /gene="ldcC"
                     /locus_tag="J1138_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBA8452240.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGSVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53457..53846
                     /locus_tag="J1138_000393"
     CDS             53457..53846
                     /locus_tag="J1138_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA8452241.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53911..55224
                     /gene="tilS"
                     /locus_tag="J1138_000394"
     CDS             53911..55224
                     /gene="tilS"
                     /locus_tag="J1138_000394"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBA8452242.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMVAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETILPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55258..55518)
                     /gene="rof"
                     /locus_tag="J1138_000395"
     CDS             complement(55258..55518)
                     /gene="rof"
                     /locus_tag="J1138_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBA8452243.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55505..55705)
                     /locus_tag="J1138_000396"
     CDS             complement(55505..55705)
                     /locus_tag="J1138_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBA8452244.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55871..56416
                     /locus_tag="J1138_000397"
     CDS             55871..56416
                     /locus_tag="J1138_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBA8452245.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56413..56835
                     /gene="arfB"
                     /locus_tag="J1138_000398"
     CDS             56413..56835
                     /gene="arfB"
                     /locus_tag="J1138_000398"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBA8452246.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56849..57559
                     /gene="nlpE"
                     /locus_tag="J1138_000399"
                     /gene_synonym="cutF"
     CDS             56849..57559
                     /gene="nlpE"
                     /locus_tag="J1138_000399"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBA8452247.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(57715..58539)
                     /locus_tag="J1138_000400"
     CDS             complement(57715..58539)
                     /locus_tag="J1138_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBA8452248.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAIKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(58592..60310)
                     /gene="proS"
                     /locus_tag="J1138_000401"
     CDS             complement(58592..60310)
                     /gene="proS"
                     /locus_tag="J1138_000401"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBA8452249.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60421..61128)
                     /gene="tsaA"
                     /locus_tag="J1138_000402"
     CDS             complement(60421..61128)
                     /gene="tsaA"
                     /locus_tag="J1138_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBA8452250.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61125..61529)
                     /gene="rcsF"
                     /locus_tag="J1138_000403"
     CDS             complement(61125..61529)
                     /gene="rcsF"
                     /locus_tag="J1138_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBA8452251.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATSVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61647..62462)
                     /gene="metQ"
                     /locus_tag="J1138_000404"
     CDS             complement(61647..62462)
                     /gene="metQ"
                     /locus_tag="J1138_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBA8452252.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62502..63155)
                     /gene="metI"
                     /locus_tag="J1138_000405"
     CDS             complement(62502..63155)
                     /gene="metI"
                     /locus_tag="J1138_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBA8452253.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63148..64179)
                     /gene="metN"
                     /locus_tag="J1138_000406"
     CDS             complement(63148..64179)
                     /gene="metN"
                     /locus_tag="J1138_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBA8452254.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFADCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64367..64939
                     /gene="gmhB"
                     /locus_tag="J1138_000407"
     CDS             64367..64939
                     /gene="gmhB"
                     /locus_tag="J1138_000407"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBA8452255.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15321 a        16122 c        17908 g        15646 t
ORIGIN      
        1 attattattt gcaatagctt aaggttattt ttcggaatat atttatattc acacgaaaaa
       61 taattaccac ttatctaatt cattaaagcc ttttgcctgt agagcttcaa ctaatgataa
      121 gccatcagga aataccaatt ccggcgcaat ttcaaacagt ggccacagca taaagccgcg
      181 atttttcata tcgtagtgcg gaacggtcag acgttcggta tttattactt cattaccaaa
      241 cagcatgatg tcgagatcca gcgtgcgcgg tccccagcgt tcagctttac ggacgcgacc
      301 ttgctgcaat tcgatacgtt gtgtgtgatt gagtagctct tcaggggcaa gagtggtttt
      361 cagcgcgacg gcggcattta agtaatcggg ttgatcttgc ggccccagcg gtggggtgcg
      421 gtaaaacgaa gaaacagcaa gaatgcggct ttctggaata tcgcctaatg ctttcagggc
      481 agcattgacc tgctccagcg gagaggccag attgctgcct atggcaatat acgccactgt
      541 catgcggtac cctcacgacg cggtgcgcgt ttgcgtggac gacgagtacg acgacgcggt
      601 gacggttctt catccagttc gttaagcatc cctttttggt ctggtggcgc ggaaacctgg
      661 aactcacccc accatttcac cagacgttgc agttcagcgt tacgctcaac ttcggctcgt
      721 aaggccaaca ggtcgtatgc tgcacggaac ttaggatgct ccagcagttt ccatgcgcgt
      781 tttccctgac gacgggacat acgcaactgc aactgccaga tatcgcgggt taacgtagtc
      841 agacgtttcg ggatcgccag tgaacggcag gcttcgtcca gcacgtcgtt catcgccagt
      901 gcgaaagcgt cgtgataggt caggccgctc tcctgggcga tcttctgggc cgtctccagc
      961 agtggatacc agaacatggc ggcaaacagg aacgccgggt tcacgcgcat atcgttatgg
     1021 atacgcgtat cggtattctt cagtacctgc tcaataatgc gttccatcgg gctgtcgcca
     1081 ttttccgtga agtagcgggt aatggtcggg aacagcggct ggaacagatg atattcacac
     1141 agcagcttat aggtttcgta accgtagccc gcttgcaaca gtttcagcga ttcttcaaac
     1201 agacgtgccg gtgggatatc gttcagtaag gttgccaggc gtgggatcgg ctcggcggtt
     1261 tccgggctga tgcgcatgcc caatttggcg gcaaaacgaa ccgcgcggag catacgtacc
     1321 ggatcttcac ggtagcgcgt ttccgggtta ccaatcagac ggataacgcc gtctttcaga
     1381 tccttcatgc cgccaacgta atcacggacg gtaaaatctg ccacgctgta atacaggctg
     1441 ttgatagtga aatcgcggcg ctgggcgtct tcttcgatgg agccgaaaat gttgtcgcgc
     1501 agcaacatgc cgttttgccc gcgttgggag gtcgtgcggt cgctgacgtt accttcgtga
     1561 tgtccacgga aggtcgcaac ttcgataatc tccgggccaa acatcacatg agccagacgg
     1621 aaacggcgac ccaccaggcg gcagttacgg aacagtttgc gcacctgctc tggcgtggcg
     1681 ttggtggtca cgtcaaaatc tttcggcttt ttgccaagta acaggtcgcg cacgccgccg
     1741 ccaaccagcc aggcttcgta tcccgcttta ttgagcctgt acattacctt cagggcattt
     1801 tcactgatat ctttgcggga aatagcatgc tgctcacgcg ggatcaccgt cacctgtgga
     1861 cgggcgactg cctgttcagc ctcgctttcc tcgcggctta gcaccttgcg gcaaaaatta
     1921 gcgactcggg taaaaatagt acacctcggt agtgtcaaac atcattcagg acaaaaaaat
     1981 agcggctaat catagctcag catgacgcat ttgagaatgt tgaatttaca attgccgact
     2041 caggcacggc ggtcagcgtc cagtttttga cggctgactg aaggatttgc tcgacgctga
     2101 aatcctgcca gtgtgtttct acctgctgac ccagaaaatg aagtgccgcg attaacaccg
     2161 ggcgcggatc gcctttcggc aacgcaggcg catgattctg cttggaaagt ttagcgcctt
     2221 gtggattaag cgccagcggt aaatggatgt aatctggcac tttccagcca aaaagctggt
     2281 acagcgagat ttgccgcacc gtcggttcga tcaaatctgc cccgcggaca atttcgctca
     2341 cgccctggaa gtgatcatcc accaccaccg ccaggttata ggcaaacaaa ccatcacggc
     2401 gatgaataat aaaatcttcc cgtgccagtt tttcgtcggc gtgaataatg cctcgcagca
     2461 gatcagtaaa ctgggtgacc ggatgctgct ggcggatacg tactgcggcg ttgtctggtc
     2521 catgatgcaa atcccggcaa tgaccgtcgt aaataccgcc aatgctttga atacgcgcac
     2581 gcgtgcaggt gcagtaataa cttagtcctt gctcatgtaa ccaggcgagt gcttcgcgat
     2641 aggcgtggtg acgttgcgat tgccagagaa cgtcgccgtc ccagtacagg ccgtaatgtt
     2701 ccagctggcg caggatagtt tctgcggcac cgggaacttc acgaggcggg tcgatatctt
     2761 caatgcgtac cagccagcga ccttgccggg cgcgagcctg caaatagctg ccaagcgcag
     2821 caatcagaga accaaaatga agctcgccgg aaggagaggg ggcgaagcgg ccaatatagt
     2881 gtgtgtctgt catctctttg aacaaaaaat aaggcgggag catttcccgc ctgtggtaaa
     2941 cgtgatggaa cggctgtaat tagccagcca tctgtttttc gcgaatttca gccagcgttt
     3001 tgcagtcgat gcacagatcg gctgtcgggc gcgcttccag acggcgaata ccaatttcaa
     3061 caccgcagga ttcgcagtag ccgaaatctt cgtcttccac ttttttcagc gtcttctcga
     3121 tctttttgat cagcttacgc tcgcgatcgc ggttacgcag ttcgaggctg aactcttctt
     3181 cctgggctgc acggtctacc gggtccggga agttggctgc ttcatcctgc atatgtgtaa
     3241 cggtgcgatc gacttcatcc ctgagttgat tacgccatgc ttccagaata cgacggaagt
     3301 gcgccagctg ggcttcattc atatactctt cgcccggctt ctcctgatat ggttccaccc
     3361 cagcgatggc gagaatactc agggacgatg ttttacggtt ttgcccttct tgcatgttgc
     3421 ttctccttaa cacgcactat cgatccccat gttcggggga aaaatgaggc cgctataaat
     3481 agcagatgct tttccggata gcaattatct aaacgtaaca cttgacaact gtgtgaggaa
     3541 aagcgtattt gcgcacgcga ccagaatgta aattaaccag ttacttactc tactacaatg
     3601 taaccggtag tgatttttta agagccatgc cttcggcaga aagttccgct ttgtaagcca
     3661 gaatttctac ccccctctgt tgagcttctg acaatagttg cgcgtatttc tcatcgatgt
     3721 ggcgcgcggg tgaaaaccgt gtaatggctg aatgcagcac ggcgaaaaag ataaccgcac
     3781 gctggccttc agccgctacg ctcatcaact cccgaaggtg tttctgacct cgttcagtga
     3841 ccgcatcggg aaaatatccc tgttcgttct ccgctaacgt aaccgatttc acttcaatat
     3901 agcagtctgg acgcgaatcc gcctgcaaca taaagtcaat acggctgcgt tcggcgccgt
     3961 attttacttc gcttttcagc gagctatagc ctgccagctc tgaaattgat tcattaagga
     4021 tagcctcttt cgtcaacctg ttagcccaaa gcgtattgac gcaaataatt gcgccgctct
     4081 ggctttgagt taattcccag gtgtgtgggt atttccgttt ggtgttgtct gaagtcgaat
     4141 accagacggt atcgccaggc gttgcacaac cggtcatcgc acccgtattc gggcagtgta
     4201 gcgttaattc gcgaccatcg ggtgtgatca catcggctaa aaaacgtttg taacgctgaa
     4261 ttagcgtcgc gcgctgtaga gggggagaaa attccattgc gacaatcctt gttattgcgt
     4321 tagcgcccag cgttttagcg gcgtgtagcg tgtgcgtcca cgggcaaacg aggaggcgta
     4381 aagggtgaac tccgtcaccg tatacgacca gttaaaacct ggcggcggga ttgtcaccgc
     4441 ctcgctggcg ccgcgcaata aggtaatatg tggatgaaac ggacgattgc tttgaaaaca
     4501 accgctgcgg gcagcctgtg agcggagcat attcgccagc tggattaagc cgcgaggcgg
     4561 ctgtcgcatc cctaaccaca ccacacgcga acgcagccat tgaccggcgt catcaagcgt
     4621 gagtgtgaaa ccaggttgac gaatccgtcc ggctaaaaga gaaagcgcct tctctttctc
     4681 tgcgctcact tcgcctaaaa atgccagagt cagatgcaaa ttatcggcgg cgaccggacg
     4741 tcccgcctca ggtgggaagt gtgtggcgcg ccagtggata atctgttcgc ggatttctgc
     4801 aggtaagtcg atagcaaaga acagccgttg cggttcagac atgtgaggca ctcggttatg
     4861 aattaccgcg atgctacaat gtggcgcgaa gaatgttaac cctctggagc gttttgtgtc
     4921 gtcgttgccc gttgctgccg tcttacctga gttacttgct gccctcgatg gtgcatcgca
     4981 ggtgttatta agtgcgccga ccggggccgg gaaatcaacc tggctgccgc tgcaactgct
     5041 ggcgcatccc ggcattaacg ggaaaattat cctgctggag ccgcgtcgtc tggcggcgcg
     5101 taacgttgcg caacgactgg cggagctgct taacgaaaag ccaggcgata ccgttggcta
     5161 ccggatgcgt gcgcaaaact gcgtcgggcc gaatacccgc ctggaagtgg ttaccgaagg
     5221 cgtgctgacg cggatgatcc agcgtgaccc ggaactgagc ggtgttggat tggtgatcct
     5281 tgatgaattt catgagcgca gcttgcaggc ggatttggcg ttggcgctgt tactcgatgt
     5341 gcaacaaggt ctgcgtgatg accttaaact gctgattatg tcggctacgc tggacaacga
     5401 ccgcttgcag caaatgctgc cagaagcgcc cgtcgtcatc tcagaagggc gctcgtttcc
     5461 ggttgaacgc cgttatttgc cgctgcccgc tcatcagcgt tttgacgaag ccgtcgcggt
     5521 tgccaccgcc gaaatgctgc gtcaggaaag tggatcatta ctgttatttt tacctggcgt
     5581 cggagaaatt cagcgtgtgc aggaacaact gacttcacgc atcggcagtg atgtgttgct
     5641 ctgcccgctg tatggcgcgt tgtcgctgaa cgatcagcga aaagcgatcc tcccggcacc
     5701 gcaagggatg cgtaaagtgg tgctggcgac caatattgct gaaacaagtt taaccatcga
     5761 aggtattcgt ctggtggtgg attgtgccca ggaaagggtg gcgcgttttg atccgcgtac
     5821 agggcttacg cggttgatta ctcaacgcgt tagtcaggcg tcgatgacgc aacgtgccgg
     5881 gcgtgccggg cgtctggagc cgggtatctg cctgcattta atcgccaaag aacaagcaga
     5941 acgcgctgcc gcacaaagtg aaccggagat cttacaaagc gatctttccg gtttgctgat
     6001 ggaattactg caatggggat gcagcgatcc ggcgcagatg agctggatgg atcaaccgcc
     6061 aacggtgaat ctactggccg cgaaacgcct gttacggatg ctgggggcgc tggacggtga
     6121 acggcttagt gcgcaagggc aaaaaatggc aacgctgggt aacgatccgc gtttagcggc
     6181 aatgctggtt agcgcgaaga acgacgacga agctgctacc gcggcaaaaa ttgccgccat
     6241 tctcgaagag ccgccacgga tgggcaatag tgacctgggc gtggcgtttt cgcgcaatca
     6301 acccgcctgg cagcaacgta gtcagcaact gttaaaacgt ttaaacgtac gcggcggtga
     6361 ggcagacagt tcgcttatcg cgccgctact tgccggagcg tatgccgatc gtattgctcg
     6421 ccgccgtggg caagagggac gctatcaact ggcgaacggc atgggggcga tgctcgatgc
     6481 cgacgatgcg ctaagccgcc acgaatggtt gatcgcaccg ttattattgc agggcagcgc
     6541 ctcgccggat gcgcggattt tactggcgct gccggtcgat attgatgagt tagtacaacg
     6601 ctgcccgcag ctggtacagc agtctgacac tgtggagtgg gatgacgcgc aaggtacgct
     6661 gaaagcctgg cgtcggctgc aaattggtca gttgatggta aaagtgcaac cgctggcgaa
     6721 accctcggaa gacgagttgc atcaggcgat gcttaacggc attcgtgata aaggtttaag
     6781 cgtgctcaac tggacggcgg aagcggaaca gctacgcttg cgtttgttat gcgccgcaaa
     6841 gtggttgccg gaatatgact ggccagcggt tgatgatgaa agtttgttgg cgacgctgga
     6901 aacgtggcta ctgccgcata tgacaggcgt acattcgcta cgcggcctga aatcactcga
     6961 tatttatcag gcactgcgtg gattacttga ttgggtaatg cagcaacgtc tggatagtga
     7021 attgcctgcg cattacactg tgccgacggg aagccggatc gccattcgtt accatgaaga
     7081 taacccgccc gcgctggcgg tgagaatgca ggagatgttt ggtgaggcca ctaatccgac
     7141 tatcgcccag gggcgcgtac cgttggtgct ggagttgctt tcccctgccc aaaggccgct
     7201 gcaaatcacg cgtgatttga gcgccttctg gaaaggagcg taccgtgagg tgcaaaaaga
     7261 gatgaaaggg cgttatccca aacatgtctg gccggacgac ccggcaaaca ccgcaccgac
     7321 gcgacgaacg aaaaagtatt cgtgaagacc aaagcaaatt tgagagatat cttcttctgt
     7381 cttgcaacag aagaacagaa aatcgggctt ttgcgcctga atattgcgga gaaaaagcat
     7441 ggccgggaat gaccgcgagc caattggacg caaagggaaa ccgacgcgtc cggtcaaaca
     7501 aaaggtaagc cgtcgtcgtt acgaagatga cgatgattac gacgattatg atgactatga
     7561 ggatgaagaa ccgatgccgc gcaaaggtaa gggcaaaggg cgtaagcctc gtggcaaacg
     7621 cggctggcta tggctgctgc taaaactggc tatcgttttt gccgtgatga tcgccattta
     7681 cggcgtttat ctcgatcaaa aaattcgcag ccgtattgat ggcaaggtct ggcaattgcc
     7741 cgcggcagtt tatggtcgaa tggtcaacct tgagccagac atgaccatca gcaagaacga
     7801 gatggtgaag ctgctggagg cgacccagta tcgccaggtg tcgaaaatga cccgtcctgg
     7861 tgagtttacc gtacaggcca acagcattga gatgatccgc cgtccgtttg atttcccgga
     7921 cagcaaagaa gggcaggtgc gcgcgcgtct gacctttgat ggcgatcatc tggcgacgat
     7981 cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt cttgatccgc gcctgatcac
     8041 catgatttct tcgccaaacg gtgagcagcg tctgtttgtg ccgcgcagtg gtttcccgga
     8101 tttgctggtg gatactttgc tggcgacaga agaccgtcat ttctacgagc atgatgggat
     8161 cagtctctac tcaatcggac gtgcggtgct ggcaaacctg actgccggac gcacggtgca
     8221 gggggcgagt acgctgacgc aacagttggt taaaaacctg ttcctctcca gcgagcgttc
     8281 ttactggcgt aaagcgaacg aagcctacat ggcgctgatc atggacgcgc gttacagcaa
     8341 agaccgtatt cttgagctgt atatgaacga ggtgtatctc ggtcagagcg gcgacaacga
     8401 aattcgcggc ttcccgctgg cgagcttgta ttactttggt cgcccggtag aagaattgag
     8461 cctcgatcag caggcgctgt tggtgggtat ggtgaaaggg gcgtccatct acaacccgtg
     8521 gcgtaacccg aaactggcgc tggagcgacg taacctggta ctacgtttgc tgcaacagca
     8581 acaaattatt gatcaagaac tctacgacat gctgagtgcg cgtccgctgg gtgttcagcc
     8641 gcgcggtggg gtgatctctc ctcagccagc ctttatgcag ctggtgcgtc aggagctgca
     8701 ggcgaaatta ggcgataagg taaaagatct ctccggcgtg aagatcttca ctacctttga
     8761 ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa
     8821 acagcgtaag ttgagcgatc ttgaaaccgc gattgtggtt gttgaccgct ttagcggtga
     8881 agttcgtgcg atggtcggtg gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca
     8941 ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact tatctaacgg ccttaagcca
     9001 gccgaaaatc tatcgtttga atacgtggat tgccgatgcg ccaatcgcgc tgcgtcagcc
     9061 gaatggccag gtctggtcac cgcagaacga tgatcgtcgt tatagcgaaa gtggcagggt
     9121 gatgctggta gatgcgttga cccgttcgat gaacgtgccg acggtgaatc tgggcatggc
     9181 gttggggctg cctgcggtta cggagacctg gattaaactg ggtgtgccga aagatcaact
     9241 gaatccagtt ccagcaatgt tgttgggggc gttgaactta acgccaatcg aagtggcgca
     9301 ggccttccag actatcgcca gcggtggtaa tcgtgctccg ctttccgcgc tacgttcagt
     9361 catcgcagaa gacggcaagg tgttgtatca gagcttcccg caggcggaac gcgctgttcc
     9421 ggcgcaggcg gcgtatctga cactatggac catgcagcag gttgtacaac gcggtacggg
     9481 tcgtcagctt ggggcgaaat acccgaacct gcatctggca gggaaaacag ggactaccaa
     9541 caataacgta gatacctggt ttgcgggcat tgatgggagc acggtgacca tcacctgggt
     9601 cggccgtgat aacaaccagc cgaccaaact gtatggtgcc agcggggcaa tgtcgattta
     9661 tcagcgttat ctggctaacc agacgccaac gccgctgaat cttgttccgc cagaagatat
     9721 tgcagatatg ggcgtggact acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt
     9781 gccggtctgg accagcgatc cgcaatcgct gtgccagcag agtgagatgc agcagcaacc
     9841 gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa cagccgcaac agcaacctgc
     9901 tcagcaagag cagaaagaca gcgacggtgt agccggttgg attaaagata tgtttgggag
     9961 taattaacgt ctaagcgtga tataccggat ggcgagctgc catccggtaa aataacatcc
    10021 cctccaaagt attaaccctt tcttttcatc tgattgttta ttgacccttc aggaatgcgc
    10081 agattgcgta ccgcttgcga acccgccagc gtttcgaata ttatctcatc tttataataa
    10141 tcattctcgt ttacgttatc attcacttta caccagagaa ataccaatgg caccttccaa
    10201 aactgctcag ccaaaacact cactgcgtaa aatcgcagtt gtagtagcca cagcggttag
    10261 cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa gaagacacta tcaccgttac
    10321 cgctgcacct gcgccgcaag aaagcgcatg ggggccggct gcaactattg cggcgcgaca
    10381 gtcagctacc ggcactaaaa ccgatacgcc gattcaaaaa gtacctcaat ctatttctgt
    10441 cgtcaccgct gaagagatgg cgctgcatca gcctaagtcg gtgaaagaag cgcttagcta
    10501 tacgccgggt gtctctgttg gtacgcgtgg cgcatccaac acctatgacc acctgatcat
    10561 tcgcggtttt gcggcagaag gccaaagcca gaataactat ctgaatggcc tgaagttgca
    10621 gggcaacttc tataacgatg cggtcatcga cccgtatatg ctggaacgtg ctgaaattat
    10681 gcgtggcccg gtttccgtgc tttacggtaa aagcagtcct ggcggtctgt tgaatatggt
    10741 cagcaagcgt ccgaccaccg aaccgctgaa agaagttcag tttaaagccg gtactgacag
    10801 cctgttccag accggttttg acttcagcga tgcactggat gatgacggcg tttattctta
    10861 tcgcctgacc ggtcttgcgc gttctgccaa tgcccagcag aaagggtcag aagagcagcg
    10921 ttatgctatt gcaccggcgt tcacctggcg tccggacgat aaaaccaatt tcaccttcct
    10981 ttcttacttc cagaacgagc cggaaactgg ttattacggc tggctgccga aagaggggac
    11041 cgttgagccg ctgccaaacg gtaagcgtct gccgacagac tttaacgaag gggcgaagaa
    11101 caacacctat tctcgtaacg agaagatgat tggttatagc ttcgaccacg aatttaacga
    11161 cacctttact gtgcgtcaga acctgcgctt tgcgcaaaat aaagtttcgc aaaagagcgt
    11221 atatggctac ggtatgtgtt cagatccgct gtataccaaa gaccaggagg cactcaaggc
    11281 gagtccatgt ttaagtatcc cgcagtctca atggggccat acactgactc gtcagtacgt
    11341 tatcgataat gagaagttag aaaacttctc tgttgatact cagttgcaaa gcaaattcgc
    11401 aaccggttct gtggatcaca ctctgctgac tggcgttgac tttatgcgta tgcgtaatga
    11461 catcgactcc tggttcggat atgccggttc agttgcacca tctgatatct ataatttaga
    11521 ccgtagtgac tttgattttg gcgctcaccc gaatccgtct ggtccatacc gcgttctgct
    11581 gaaacagaaa caaaccggcc tgtatgttca ggatcaggcg cagtgggata aagtgctggt
    11641 tacgcttggc ggtcgctatg actgggccga tcagtcatct ttcaaccgtg attacggtaa
    11701 taaatctgaa cgtgacgaca aagagttcac ctggcgtggt ggtgtaaact acctgttcga
    11761 caacggcgta acgccttact tcagctacag tgaatcattt gaacccgctt cacagactga
    11821 tgcgaacggt gatctgtttg caccttcgaa aggcaaacag tatgaagtcg gcgtgaaata
    11881 tgtgccagaa gatcgcccaa ttgtggtgac tggcgcactg tatcagctta ccaaaactaa
    11941 taacctgatg gcggatccga acggttcact gttctcggtt gaaggcggtg agattcgtgc
    12001 gcgtggcgta gagctggaag cgaaagcggc actgtcggcg agtgttaact tggttggttc
    12061 ttacacctat actgacgcgg aatacaccac tgacacaaac tacaaaggca atacgcctgc
    12121 acaggtgcca aaacacatgg cttcgctgtg ggctgactat accttctttg acggtccgct
    12181 ttcaggtctg acgctgggca ccggtggtcg ttatactggc tccagctatg gtgatccggc
    12241 taactccttt aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt atgatctggc
    12301 gcgagtcggc atggcgggct ccaacgtggc gctgcatgtt aacaacctgt tcgatcgtga
    12361 atacgtcgcc agctgcttta acacttatgg ttgcttctgg ggcgcagaac gtcaggtcgt
    12421 tgcaaccgca accttccgtt tctaatttct cttttggggc acggatttcc gtgcccgttt
    12481 cacaagttgg ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt
    12541 aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc cgttgtcgtt aacctttcct
    12601 gccgggaaag tgactggtct gattggtcac aacggttctg gtaaatccac tctgctcaaa
    12661 atgcttggtc gtcaccagcc accgtcggaa ggggagattc ttcttgatgc ccaaccgctg
    12721 gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt atttaccgca gcagcttcct
    12781 ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc
    12841 gcactagggc gctttggtgc ggcagatcgc gaaaaggtcg aggaagctat ctcgctggtt
    12901 ggcttaaaac cgctggcgca tcggctggtc gatagcctct ctggcggcga acgtcaacgg
    12961 gcgtggatcg ctatgctggt ggcgcaggat agccgctgtc tgttgctcga cgaaccgacc
    13021 tcggcgctgg atatcgccca ccaggttgat gtactggcgc tggtgcatcg tttaagtcag
    13081 gagcgaggcc tgacggtcat tgccgtgctg cacgatatca atatggcggc gcgctactgt
    13141 gattatctgg tcgccctgcg cggcggtgaa atgattgctc agggaacgcc tgcggaaatt
    13201 atgcgcggcg aaaccctcga aatgatttat ggcatcccga tgggtattct gccgcatccg
    13261 gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc
    13321 gccgactgtt aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg
    13381 cggcagttat tgatcccaat cgtattgtgg cactggagtg gttgccggtg gagttactgc
    13441 tggcgctcgg catcgtgcct tacggcgtgg cggacaccat caactatcgc ctgtgggtca
    13501 gcgaaccgcc attgccggac tcagtgattg acgttggttt gcgtacagaa cccaaccttg
    13561 aactgctgac cgaaatgaaa ccgtcgttta tggtctggtc tgcaggatat ggcccttcac
    13621 cagaaatgct ggcacgtatt gcgccggggc gcggatttaa cttcagcgac ggcaaacagc
    13681 cactggcgat ggcgcgtaaa tcactgacgg aaatggcaga tttacttaac ctgcaaagcg
    13741 cagcggaaac gcatttagcg cactatgaag actttatccg cagcatgaaa ccccgctttg
    13801 tgaagcgtgg tgcacgcccg ttattgctga cgacgcttat cgatccgcgc catatgttag
    13861 tcttcggtcc aaacagcttg ttccaggaaa ttctcgatga gtacggcatc ccaaatgcct
    13921 ggcaagggga aaccaacttc tggggcagta ccgccgtcag tatcgatcgt ctggcagtgt
    13981 ataaagacgt tgatgtgctc tgttttgatc acgacaacag caaagacatg gatgcgctaa
    14041 tggcaacccc gctgtggcag gccatgccgt ttgtccgcgc cggacgcttt cagcgcgttc
    14101 cggcggtctg gttctatggt gcgacgctct cggcaatgca ctttgtgcgt attctggata
    14161 acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt tttccggtgt tattgctggc
    14221 gctgctggtg gttgctgctg cggcgttgac ctggatgaac ttctcgcagg cgctgccgcg
    14281 cagccagtgg gcgcaggccg cctggtcgcc ggatattgac gtcattgagc agatgatttt
    14341 tcactacagc ttgttgccgc gtctggcgat ttcgctgctg gtgggcgcgg gcctggggct
    14401 ggtgggcgtg ctgtttcagc aagtgctgcg taacccgctg gcggaaccga cgacgttggg
    14461 tgttgcaaca ggcgcgcaac tggggattac cgtcaccacg ctctgggcga tccccggcgc
    14521 gatggcgagc cagtttgctg cgctggcagg ggcttgtgtt gttggcttaa tcgtctttgg
    14581 cgtcgcgtgg gggaaacggc tttcgccggt aacgctgatc ctcgcggggc tggtagtgag
    14641 cctttattgc ggcgcaatca atcagttact ggttatcttc catcatgacc aactgcaaag
    14701 catgttcctg tggagcaccg gaacgctgac gcaaaccgac tggggcggcg ttgagcgttt
    14761 atggccgcag ctgctgggcg gcgtgatgct gacgttattg ctacttcgcc cgttaactct
    14821 gatggggctt gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc tcgctcgtct
    14881 ggcggcgttg tcgctggcga ttgtcatcag tgcgctgctg gtgaacgcgg tggggattat
    14941 cggctttatc gggttgttcg cgccactgct ggcgaaaatg ctgggggcgc ggcgtttgtt
    15001 gccacggttg atgctggcat cgctgattgg tgcgttaatt ctgtggcttt ctgatcaaat
    15061 catcctctgg ctgactcgcg tgtggatgga agtgtccacc ggttcggtca ctgcgttgat
    15121 cggtgcgccg ctgctactgt ggttgctgcc gcgtttacgc agcattagcg cgccggatat
    15181 gaaggtgaac gatcgtgtcg cgactgaacg ccaacacgtg ctggcgtttg ccctcgcggg
    15241 cggcgtgctg ctgttgatgg ctgtggtagt ggcgctgtcg tttggtcgtg atgcgcacgg
    15301 ctggacgtgg gcgagcgggg cgttgctcga ggatttgatg ccctggcgct ggccgcgaat
    15361 tatggctgcg ctgtttgcgg gcgtcatgct ggcggtggcg ggctgtatta ttcagcggct
    15421 gaccggaaac ccgatggcaa gcccggaagt gctggggatt agctccggcg cggcgtttgg
    15481 cgtggtgtta atgctgtttc tggtgccggg taatgccttt ggctggctgt tacccgcagg
    15541 cagtctcggg gcggcggtga cgctgttgat cattatgatc gccgccggac gcggtggatt
    15601 ttccccacac cgcatgttac tggcggggat ggcgttaagt accgcgttca ccatgctttt
    15661 gatgatgttg caggcaagtg gtgacccgcg aatggcgcaa gttctgacct ggatttccgg
    15721 ttcgacctac aacgcgaccg atgcgcaggt ctggcgtacc ggaattgtga tggtgatttt
    15781 gctggcgatt accccgctgt gccgccgctg gctgaccatt ttaccgctgg gtggtgatac
    15841 cgcacgagcc gtggggatgg cgctgacgcc gacgcgaatt gcgctgctgc tgttagcggc
    15901 ttgcctgacg gcgaccgcga caatgaccat tggaccgttg agttttgttg gtttaatggc
    15961 accgcatatt gcgcggatga tgggctttcg acggacgatg ccacacatcg taatttcggc
    16021 gctggtgggt ggtttactgc tggtgttcgc tgactggtgt gggcggatgg tattgtttcc
    16081 attccagatc ccggcggggc tgctgtcgac ctttatcggc gcgccatatt ttatctattt
    16141 gttgagaaag cagagccgtt aatagaatgc ctcgcccggt gggcgaggca aaatcattac
    16201 aacttcgcaa acacccgacg tgcagcatcg atggtgttat tgatatcttc catgctatgc
    16261 gccacggaca taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc ttcgtccagc
    16321 atcatatgga agaaacgctt aaagcgttcc acgtcacagg ccatcacatc ctgatagcac
    16381 gtcacggact cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg gtttaccacc
    16441 agcggaattc cggcttcttc tgccgcttcc cgcagacctt ctgccagacg tgttgtcagc
    16501 tcatccagcg tctcgtgaac gcccggctgt gcgacttcat tcagacaggc gaaaccagca
    16561 gccatcgcaa tcgggttacc ggaaagcgta cccgcctgat agaccggacc ggtcggggcc
    16621 agcgcatcca ttacatcacg acgaccaccg aatgcgccta ccggcattcc accgccgatg
    16681 attttgccga ggcaggtgag atccggttcc acaccgtaat aatcctgtgc gccagctagc
    16741 gctacgcgga aaccggtcat cacttcatcg atgatcagca acgcgccaaa ttcgtcgcac
    16801 agcgcgcgca gacctggcag gaactctggc agcggtggaa cgcagttcat attgcctgcc
    16861 accggctcga cgataataca ggcaatctct tgcgggtatt gctcaaacgc ggcgcgtaca
    16921 gaggccagat cgttataagt acaggttaag gtatgtttgg cgaaatctgc cggaacgccc
    16981 ggcgaatttg gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag caggcagtca
    17041 gcgtgaccgt ggtaacaacc ttcaaattta ataattttgt cgcgaccggt aaaaccacgg
    17101 gccaggcgga tggcgctcat cgtcgcttca gtgccggagt tcaccatgcg caccatatcc
    17161 atggtcggga ccagttcggt caccagttgc gccattttca cttccatttc ggttggtgca
    17221 ccaaagctta aaccacgctc ggcggcttca atcacggcat tgcggatcgc cggatggtta
    17281 tggcccagca ccatcggccc ccaggaaccg acataatcga tataggcttt gccatcaaca
    17341 tcgtacagat aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc cacgccagta
    17401 aaggcgcgaa caggggagtt cacaccgcca gggatcagct cgcgcgctgc gctgtaaaga
    17461 ttttcagact tactcatgga gggttcctga ttcgtagaaa aagtgaatgg ctgctattct
    17521 atgttattca taacaagtta aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc
    17581 ctgatttctt agagtataaa agttttgtgc atttgaaaca ttacgctttg caaaggattt
    17641 tcatggaacg tgcgagtaaa atgccgtcat cttatttgta tgaccaataa gtgatcattg
    17701 gatgaaaact gatactccct ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca
    17761 actgattcgc caacttcttg agcgcgataa aaccccgtta gccattttgt ttatggcggc
    17821 agtcgtcggc acgcttgtcg ggctggctgc ggttgctttt gacaaaggtg tcgcctggtt
    17881 gcagaaccaa cgtatggggg cactggtaca tactgctgat aattatccgc ttctgttaac
    17941 cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc tactttttgg tgcgcaaata
    18001 cgcgccggaa gcaggtggtt cggggatccc ggaaattgaa ggggcgctgg aagatcaacg
    18061 tcccgttcgc tggtggcgtg tgttgccggt gaaattcttt ggcgggctgg gaacactcgg
    18121 cggaggcatg gtgttggggc gcgaagggcc aaccgtgcag ataggcggta atatcgggcg
    18181 gatggtactc gatgttttcc gcctgaaagg tgacgaagct cgtcatacgc tactggcaac
    18241 cggtgctgct gcggggctgg ctgcggcctt taacgcgccg ctggcgggta ttttgtttat
    18301 tatcgaagag atgcgtccgc agtttcgcta tacgttaatt tcgattaaag cggtatttat
    18361 tggcgtcatt atgtcgacca ttatgtaccg gatttttaat catgaagttg cgttgattga
    18421 cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg ctttatctga tcctcggtat
    18481 tatttttggc attttcggcc ctatttttaa taaatgggtg ctggggatgc aggatttgct
    18541 gcaccgtgtg cacggtggca atattaccaa atgggtactg atgggcggtg cgattggcgg
    18601 tctgtgtgga ttgctggggt ttgtggcacc agcaacttcg ggcggcggtt ttaacctgat
    18661 tcctatcgct accgcgggga atttcagcat gggaatgctg gtgtttatct tcgtcgcgcg
    18721 ggtcattacc actttactct gcttctcttc cggcgcgccg ggcggtattt ttgccccgat
    18781 gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg agttgtttcc
    18841 gcaatatcac cttgaggcgg ggacgtttgc tattgccgga atgggggcgt tactggcggc
    18901 atctattcgc gcgccgttaa cggggatcat tctggttctg gaaatgaccg ataactacca
    18961 gctcattttg ccaatgatta ttaccggtct tggcgcaaca ctattagcac aatttaccgg
    19021 tgggaaaccg ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg aagctgagca
    19081 actggcgcga agcaaggccg catcagcccg cgagaatact tgaacgaaat accagggtat
    19141 tagataatgg cgattattat tgggttagaa tttgcccaat tgccgatgtc gtttggagca
    19201 aaatatgagt gatgacgtag cactgccgct ggagtttacc gacgcagcag ccaacaaagt
    19261 taaaagcctg atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg
    19321 tggcggttgc agcggcttcc agtatggttt cacctttgat gatcaggtga acgaaggcga
    19381 tatgaccatc gaaaaacagg gcgttggcct ggtggttgat ccgatgagcc tgcaatatct
    19441 ggtcggcggt tccgttgact ataccgaagg tctggaaggt tctcgtttca tcgtgaccaa
    19501 cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt agtatctaat ctgctgttca
    19561 tgtgccggat aaactatccg gcacatcttc tctcttcaac gaccattctc atccagcgca
    19621 aacgtcggta gcttaagatg ccaacgaata gccgccagcc gaatcaatag cgtcacgacc
    19681 atgcccatca tactggctgt ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg
    19741 acaataccgc cgataataca ggcagttgcg tagatttctg tacgtaaaat catggggatt
    19801 tcgcgggcca gtacatcacg aatgatcccg ccgccaacgc cagtaatgac gcccatacaa
    19861 accgcgatta acggaccggc ttccgcatta aaggctttat tcacgccaat gccgacaaac
    19921 accgccagac caacggcgtc cagcaccggc aacatccatt ttggtaagcg tcttggctgg
    19981 cgcaccagca cgatggtcag catgctggtg accattgcaa cgaccagatc ggtgggatct
    20041 ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc gaattgttcc gccgcctact
    20101 gcggtaacca cgcccaatac cagaacacca aaagggtcca tacgcaattt tccggctaac
    20161 aaaacgccag agatagcaaa taccgctgtg ccgactatat ccagccaata gacgagcatt
    20221 gttcaatccc cactgagcac ctgaaaaggt caggcactaa tctacctgtg aaagcgcatt
    20281 acagagctgt tgtgcagcga ggataatacg tgggcttgca cgttcaaacc agtcactcgt
    20341 gagaggaata acgggaattt tgagctgttc accccagtat tgtttgattt taggaatttg
    20401 gtccggtccg cctgtaatga caatcgcctg tggcgagcgt gctaacacct gttcgcggct
    20461 aacttgcggc cagggaaccc ggctgtcttt aaagatgttt tctccgccac aaacttcgag
    20521 tacctggttc tgaatcgact cttttccact ggtaaatggc ggattaatgc cgaattgcag
    20581 aaaaacacgt tttttaggtt tatcagcata ttgcgctttc aattgcgcgt actgatccag
    20641 cagggattgc gcggcttgtt cggccttgtc tggttgcgga ctccaggggg ccagttgacg
    20701 taacgcattg gcaatttgtt caatgcttgt cgcatcgacc cacatcactt ttattcccag
    20761 cgaagccagc tggtcaacct gccgctcggc attacctcca cgccaggcaa tcaccagatc
    20821 gggtttcagc gcgacaatgc gttccagatt catcccctgc caggtggaaa cctgttcaat
    20881 cttttgcgct tgtggaggat agtcggaata gctgctgacc ccaaccggcg tgatcccggc
    20941 ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg cggcgttgag
    21001 ccacagcggc gcgagaaaag acagggcgac tagcgccctg aacagtgact tagccatgtg
    21061 ccagtttctg caccagtgac tcaaccatca ggctggactg tttagcggca acagccagga
    21121 actcatcgaa gctaagatga gactgttgat cggccacgtc ggagatggcg cgtactacga
    21181 caaacgggac gttgaaattg tggcagacat gggcgattgc cgtcgcttcc atctctacag
    21241 caatggcctg tgggaaattg tggcggattt tcgccagacc aacagaacca ttgatgaaag
    21301 cgtcgccgct aacaatcagg ccacgtacag cgtttagatt cagttcggca atgcaggcct
    21361 cagcggcagc gatcagttta tcgtcagctt taaagcctgc cggacagcct ggtaactgac
    21421 cgtattcata accaaatgcc gtgacatccg cgtcgtgata acgcgcttcg tccgagacaa
    21481 cgatatcgcc cactttcagc gttggtgcca ggccaccggc agaaccggtg ttaataatca
    21541 catctggctt gcagtgttcc aacagcaaag tggcacccag cgccgcagcg actttaccga
    21601 tgcccgattt cagaagcgca acctcggttc cattcagttg gccggtatag atttcgcaac
    21661 cgcccagact gatagtttga cggttttcga ttttgtcacg cagcagcgta acttcttctt
    21721 ccattgcacc aatgatgccg attttcatag atttactcgc gataagcccg atttgaaggc
    21781 atagtttacc atgcgcttac ggggaagcgt atttctcacg cgggagagga catgacacag
    21841 attgatttcc gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca gggcgttaaa
    21901 accgaacatg agattctgcg gatcttcgag agcgatcgcg ggcgtatcat caactctccg
    21961 gcaattcgtc gtctgcaaca aaagacccag gtttttccac tggagcgcaa tgccgccgtg
    22021 cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg ggcgctacat cgccaaagaa
    22081 attttaagcc gtctgaaaga gcttaaatta ctggaggcgt atggtctcga tgaactgacc
    22141 gggccctttg aaagcattgt tgagatgtca tgcctgatgc acgatatcgg caatccgccg
    22201 tttggtcatt ttggcgaagc ggcgataaat gactggtttc gccagcgttt gtacccggaa
    22261 gatgccgaaa gccagcctct gactgacgat cgctgcagcg tggcggcact acgtttacgg
    22321 gacggggaag aaccgcttaa cgcgctgcgg cgcaagattc gtcaggactt atgtcatttt
    22381 gaaggtaacg cacagggtat tcgtttggtg catacattga tgcggatgaa tctcacctgg
    22441 gcacaggttg gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg tgaaacgcct
    22501 gagacacatc actatttaat gaaaaagccg ggctattatc tttccgaaga agcctatatt
    22561 gcccggttgc gtaaagaact taatttggcg ctttacagtc gttttccgtt aacgtggatt
    22621 atggaagctg ccgacgacat ctcctattgt gtggcagacc ttgaagatgc ggtagagaaa
    22681 aggatattta ccgttgagca gctttatcat catttgcacg aggcgtgggg ccagcatgag
    22741 aaaggttcgc tcttttcgct ggtggttgaa aatgcctggg aaaaatcacg ctcaaatagt
    22801 ttaagccgca gtacggaaga tcagtttttt atgtatttac gggtaaacac cctaaataaa
    22861 ctggtaccct acgcggcaca acgatttatt gataatctgc ctgcgatttt cgccggaacg
    22921 tttaatcatg cattattgga agatgccagc gaatgcagcg atcttcttaa gctatataaa
    22981 aatgtcgctg taaaacatgt gtttagccat ccagatgtcg agcagcttga attgcagggc
    23041 tatcgggtca ttagcggatt attagagatt tatcgtcctt tattaaacct gccgttatca
    23101 gactttactg aactggtaga aaaagaacgg gtgaaacgtt tccctattga aacgcgctta
    23161 ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag taaattaccg
    23221 tcagattctc ctgagtttcc gctatgggaa tattattacc gttgccgcct gctgcaggat
    23281 tatatcagcg gtatgaccga cctctatgcg tgggatgaat accgacgtct gatggccgta
    23341 gaacaataac caggcttttg taaagacgaa caataaattt ttaccttttg cagaaacttt
    23401 agttcggaac ttcgcgctat aaaacgaatc tgaagaacac agcaattttg cgttatctgt
    23461 taatcgagac tgaaatacat gaaaaaaacc acattagcac tgagtgcact ggctctgagt
    23521 ttaggtttgg cgttatctcc gctctctgca acggcggctg agacttcttc agcaacgaca
    23581 gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc agtggtcagc
    23641 attaacgtag aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa tttccagcag
    23701 ttcttcggtg atgattctcc gttctgtcag gagggttctc cgttccagag ctctccgttc
    23761 tgccaggggg gcctgggcgg taatggcggc ggccagcaac agaaattcat ggcgctgggt
    23821 tccggcgtta tcattgatgc cgataaaggc tatgtcgtca ccaacaacca cgttgttgat
    23881 aacgcgacgg tgattaaagt ccaactgagc gatggtcgta agttcgacgc gaagatggtt
    23941 ggcaaagatc cgcgctctga tatcgcgctg atccagatcc agaacccgaa aaacctgacc
    24001 gcaattaaga tggcggattc tgatgcgctg cgcgtgggtg attacaccgt agcaattggt
    24061 aacccgttcg gcctgggcga gacagtaact tccgggattg tctctgcgct gggacgtagc
    24121 ggcctgaacg ctgaaaacta cgaaaacttc atccagactg atgcagcgat caaccggggt
    24181 aactcaggtg gtgcgttggt taacctgaac ggcgaactga ttggtatcaa caccgcgatt
    24241 ctcgcaccgg acggcggaaa catcggtatc ggttttgcta tcccgagcaa catggtgaaa
    24301 aacctgacct cgcagatggt ggaatacggc caggtgaaac gtggtgagtt gggtattatg
    24361 ggcactgagc tgaactccga tctggcgaaa gcgatgaaag ttgacgctca gcgcggtgct
    24421 ttcgtaagcc aggttctgcc gaattcttcc gccgcgaaag cgggcattaa agcgggtgat
    24481 gtgatcacct cactgaacgg taagccaatc agcagctttg ccgcactgcg tgctcaggtg
    24541 ggcactatgc cggtaggtag caaactgacc ctgggcttac tgcgcgacgg gaagcaggtt
    24601 aacgtgaacc tggaacttca gcagagcagc cagaatcagg ttgattccag caccatcttc
    24661 aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt ggtggtgaac
    24721 aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga tgtgattatt
    24781 ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagttct cgacagcaaa
    24841 ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca ccatctacct gttaatgcag
    24901 taatctccct caaccccttc ctgaaaacgg gaaggggttc tccttataat ctgtgaactt
    24961 caccacaact ccatacatct tcatcatcct ttgggcattt gcacaatgcc gtacgttacg
    25021 tacttcctta tgctaagccg tgcataatgg aggacttatg gctggcatct tgataccaaa
    25081 atggcgcagg atatcgtggc acgtaccatg cgcatcatcg ataccaatat caacgtaatg
    25141 gatgcccgcg ggcgaattat cggcagcggc gatcgtgagc gtattggtga attgcacgaa
    25201 ggagcattgc tggtactttc acaaggacga gtcgtcgata tcgatgacgc ggtggcacgt
    25261 catctgcacg gtgtgcggca ggggattaat ctaccgttac ggctggaagg tgaaattgtc
    25321 ggcgtaattg gcctggcagg tgaaccagag aatctgcgta aatatggcga actggtctgc
    25381 atgacggctg aaatgatgct ggaacagtcg cggttgatgc acctgttggc gcaggatagc
    25441 cgtttgcggg aagaactggt gatgaacctc attcaggctg aggagaatac tcccgcactt
    25501 actgaatggg cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt
    25561 gaggtcgaca gcggtcagct tggtgtggac agcgcaatgg cggagttaca gcaactgcaa
    25621 aatgcgctga ctacgcccga gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg
    25681 gtggtgttga aaccggcgtt gaactctttt gggcgctggg acgcagaaga tcatcgtaag
    25741 cgagttgaac aactgattac ccgcatgaaa gagtatggcc agttgcgttt tcgcgtttcg
    25801 ctgggcaact attttaccgg tcctggcagt attgcccgat cctatcgtac ggcgaaaacg
    25861 acgatggtgg tggggaaaca gcggatgcca gaaagtcgct gctattttta tcaggatctg
    25921 atgttacctg tgttactcga cagtttgcgt ggcgactggc aggccaacga actggcgcga
    25981 ccgctggcgc ggctgaaagc gatggacaat aacggcttgc tgcggcgaac gctggcggcg
    26041 tggtttcgcc acaatgtgca accgctggca acgtcaaagg cgttgtttat tcatcgtaat
    26101 actctggaat atcggcttaa tcgtatatcg gaactgaccg ggcttgattt gggcaatttt
    26161 gatgacaggt tgctgctgta tgtggcgtta caactggatg aagagcggta ggtcgattac
    26221 aaaattacag ccgggtgaga ctttacccgg ccacagaatg gcattattta cgcgtcagtt
    26281 tttccagatc ggcttcaatc tcgctgatct tattggttac tactgactcc aggtgacgta
    26341 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
    26401 caagctcatc aatgatatac cgtagattcg ggctaatttc ctggacttct ttataaccct
    26461 gacccacacc atcagcgacg accgttttac gctgacgcgg gtatttaaac ttcacgctct
    26521 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
    26581 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
    26641 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
    26701 ctggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
    26761 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata ttctatggga tggattagca
    26821 catttatctt cattttgcga attacatgac agtccgaaac aagatgaaaa agtatttaaa
    26881 aaaaaggggg aggaggaaga atgagtagga cgaattattc tgagccagaa agcaatcggg
    26941 cagcgtcgtg ctgcccgtgt acatactttt agtcgatggt acgcagcagt tcgttaatac
    27001 caactttgcc gcgagttttc gcgtcaactt tcttaacgat aactgcacag tagaggctgt
    27061 atttgccatc tttcgacggc agattacctg aaacaaccac agaccctgcc ggaacgcgac
    27121 cgtagtggat ttcgccggtt tcacggtcat aaatacgggt gctctgacca atgtatacgc
    27181 ccatagagat gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc
    27241 cgatgaagca gttatcttca ataatggtcg ggttagcctg cagcggctcc agcacgccgc
    27301 cgatacccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacag gaaccgacgg
    27361 tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg
    27421 gcatcagcac ggtgttacgg gcaatgaacg cgccctgacg cactgccgct ggtggcacga
    27481 cgcggaagcc ttctttctgg aaacgtgctt catcgtagtt agcgaatttc atcggcactt
    27541 tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga
    27601 gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta
    27661 cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg
    27721 tgtctgcatt ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct
    27781 gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg
    27841 gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagcgcac
    27901 gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc
    27961 gggcgccatg aagcgaaatt cccagatcgg caaaaatttt cccgactcgc gccagcagtc
    28021 caggttggtc gagggcgatc agttcgagga acgatttgcg gtcggtatgg gtcggcaaaa
    28081 acgtcacttc ggtttcaaca gtaaaatggc gcaatttggc aggttggcga cgcggctgcg
    28141 gtggctgcca gctactttgt gtcagcacct gctccagacc aaaccgaata acctcatgac
    28201 gatcggcgga cagcgggctg ccatcgggtt ccagtacgat gaaggtatcc atcgccatac
    28261 cgtcacgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg
    28321 cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc
    28381 cacgcgtagc ctgcgggcta agcagtacca gcggtttgct taaatcatgc tgtaataaat
    28441 ggcgtgcgtg ccaggccagt tgatttgggc tatggcggac aaaatagtta gcgcgacagc
    28501 gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagcg
    28561 cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc
    28621 gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc
    28681 acagtgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga tagcgcagac
    28741 gattttccgt ttgaacttct tcggcaaact gcttgatgac ttccgggtcc tgaatatcgc
    28801 ggcgctgagc tgtcaccgac atcagcaagt gctggcgaac cagccaggcg accagctgtg
    28861 tttcgcggga gttcagcccg tggagttcgg caaaatgcac tacatcctga gcaccgagaa
    28921 tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca
    28981 gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt
    29041 cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt
    29101 aggcatggaa caggtcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg
    29161 cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac
    29221 gcagaatgct caaaaacagt tttcgcgctt ccggaatatt acacagaggt tgttgcaggt
    29281 ggcgacgggc gtggcgtaac tggcgtagtg tggtggagta aatgccggtg atcgcactgt
    29341 tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt
    29401 catcacgcag gtcgatcagc gttccgcgta gctgaaactc atcgtcgatt ggacgtggtt
    29461 tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga
    29521 gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt
    29581 taccttcacc actataattc aaacgctggg cgacgctaag ctggcgatcg aataacaggc
    29641 gattatcgta acgactgacg accagatgca gggcaaagcg aatgcgccac aatatatgca
    29701 ggcattcgtt taattccgct ctctccgcag aggttaagaa gccaaaccct accatttcat
    29761 ccagcgatgt tgctccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca
    29821 agccgccggg gctacttttg atgtctggtt caaggttgta gctggtgccg tggtaacgct
    29881 gatggcgctg gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc
    29941 cttcgctaaa aatatgtttt tgcagttcga ggaacagcgc aacatcgcca attaataagc
    30001 gggattcgat taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt
    30061 caagcgtgcg cacgctatga cctacttcca gctttacatc ccagagcagc gttaacagct
    30121 cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga
    30181 tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt
    30241 cggcaatctg gctgaatccc gcttcaatcc ataatcgttg aagaagctgg tcgataaact
    30301 cggtacgcgc ctcaatcaac tgttctgcgg agataccatt gtcaaaggca tcacccagcc
    30361 aacgctggaa agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc
    30421 aggcgcatgg attttgcggt tgaccgtgca gggtggggag agcggtgttt gcgtactgtt
    30481 ctggaagggt attcattgtg cgccacccat aagattaaat tattgcatta aaaaagccgg
    30541 cattcgccgg cttcatttta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg
    30601 cgtagcgtca gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca
    30661 gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt
    30721 ttacccgcgt tgaccattgg ctcgatggtg aacgtcatcc ccggtttcag cacgacgttg
    30781 gtttcacggg agtcatagtg cagcacctgc ggttcttcgt ggaagccgcg accaataccg
    30841 tgtccacaat attcacgaac gacggagaag ccttctgctt cgacaaattt ctgaattgcc
    30901 gcaccgattt cgcgcagatt aatgccaggt tttaccatgc gtagcgccag gtacaggctt
    30961 tcttgcgtga tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt
    31021 ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg
    31081 ccatctttca gcagcttggc gtcgtccggg ataccgtggc acaccacttc attaatagag
    31141 atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca
    31201 ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca
    31261 tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg
    31321 atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac
    31381 cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc
    31441 tgaatttcgc caaacgtgcc actgaaggtt ttctataata gaaaattcaa cgtctgatgc
    31501 tgtacacagc gccaacaatt attggtgtcc acgacgtatt tgtggtataa agcgcgccgg
    31561 acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac
    31621 aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg
    31681 tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc
    31741 gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg
    31801 aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta
    31861 ccgatgttca acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc
    31921 cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc
    31981 gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt
    32041 cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgac
    32101 aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc
    32161 ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac
    32221 cacgaacaca ttgctatcaa agaagcaaac aacctgggta ttccagtatt tgctatcgtt
    32281 gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc
    32341 cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag
    32401 gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg cacgctcgta
    32461 cgagccctta tagaccaggt agtacacgtt tggttagggg gcctgcatat ggcccccttt
    32521 ttcactttta tatctgtgcg gtttaatgcc gggcagatca catctccgag gattttagaa
    32581 tggctgaaat taccgcatcc ctggtaaaag agctgcgtga gcgtactggc gcaggcatga
    32641 tggattgcaa aaaagcactg actgaagcta acggcgacat cgagctggca atcgaaaaca
    32701 tgcgtaagtc cggtgctatt aaagcagcga aaaaagcagg caacgttgct gctgacggcg
    32761 tgatcaaaac caaaatcgac ggcaactacg gcatcattct ggaagttaac tgccagactg
    32821 acttcgttgc aaaagacgct ggtttccagg cgttcgcaga caaagttctg gacgcagctg
    32881 ttgctggcaa aatcactgac gttgaagttc tgaaagcaca gttcgaagaa gaacgtgttg
    32941 cgctggtagc gaaaattggt gaaaacatca acattcgccg cgttgctgcg ctggaaggcg
    33001 acgttctggg ttcttatcag cacggtgcgc gtatcggtgt tctggttgct gctaaaggcg
    33061 ctgacgaaga gctggttaaa cacatcgcta tgcacgttgc tgcaagcaag ccagaattca
    33121 tcaaaccaga agacgtatcc gctgaagtgg tagaaaaaga ataccaggta cagctggata
    33181 tcgcgatgca gtctggtaag ccgaaagaaa tcgcagagaa aatggttgaa ggccgcatga
    33241 agaaattcac cggcgaagtt tctctgaccg gtcagccgtt cgttatggaa ccaagcaaaa
    33301 ctgttggtca gctgctgaaa gagcataacg ctgaagtgac tggcttcatc cgcttcgaag
    33361 tgggtgaagg catcgagaaa gttgagactg actttgcagc agaagttgct gcgatgtcca
    33421 agcagtctta attatcgaaa aggagccgcc tgagggcggc ttctttttgt gcccatcttg
    33481 taaattcagc taacccttgt ggggctgcgc tgaaaagcga cgtacaatgt cgctagtatt
    33541 aattcatttc aatcgttgac agtctcagga aagaaacatg gctaccaatg caaaacccgt
    33601 ctataaacgc attctgctta agttgagtgg cgaagctctg cagggcactg aaggcttcgg
    33661 tattgatgca agcatactgg atcgcatggc tcaggaaatc aaagaactgg ttgaactggg
    33721 tattcaggtt ggtgtggtga ttggtggggg taacctgttc cgtggcgctg gtctggcgaa
    33781 agcgggtatg aaccgcgttg tgggcgacca catggggatg ctggcgaccg taatgaacgg
    33841 cctggcaatg cgtgatgcac tgcaccgcgc ctatgtgaac gctcgtctga tgtccgctat
    33901 tccattgaat ggcgtgtgcg acagctacag ctgggcagaa gctatcagcc tgttgcgcaa
    33961 caaccgtgtg gtgatcctct ccgccggtac aggtaacccg ttctttacca ccgactcagc
    34021 agcttgcctg cgtggtatcg aaattgaagc cgatgtggtg ctgaaagcaa ccaaagttga
    34081 cggcgtgttt actgctgatc cggcgaaaga tccaaccgca accatgtacg agcaactgac
    34141 ttacagcgaa gtgctggaaa aagagctgaa agtcatggac ctggcggcct tcacgcttgc
    34201 tcgtgaccat aaattaccga ttcgtgtttt caacatgaac aaaccgggtg cgctgcgccg
    34261 tgtggtaatg ggtgaaaaag aagggacttt aatcacggaa taattcccgt gatggataaa
    34321 taagggtaag attccgcgta agtatcgcgg gggatcttac ccgtaatatg tttaatcagg
    34381 gctatactta gcacacttcc actgtgtgtg actgtctggt ctgactgaga caagttttca
    34441 aggattcgta acgtgattag cgatatcaga aaagatgctg aagtacgcat ggataaatgc
    34501 gtagaagcgt tcaaaaccca aatcagcaaa atacgcacgg gtcgtgcttc tcccagcctg
    34561 ctggatggca ttgtcgtgga atattacggc acgccgacgc cgctgcgtca gctggcaagc
    34621 gtaacggtag aagattcccg tactctgaaa atcaacgtgt ttgatcgttc aatgtctccg
    34681 gccgttgaaa aagcgattat ggcgtccgat cttggcctga acccgaactc tgcgggtagc
    34741 gacatccgtg ttccgctgcc gccgctgacg gaagaacgtc gtaaagatct gaccaaaatc
    34801 gttcgtggtg aagcagaaca agcgcgtgtt gcagtacgta atgtgcgtcg tgacgcgaac
    34861 gacaaagtga aagcactgtt gaaagataaa gagatcagcg aagacgacga tcgccgttct
    34921 caggacgatg tacagaaact gactgatgct gcaatcaaga aaattgaagc ggcgctggca
    34981 gacaaagaag cagaactgat gcagttctga tttcttgaac gacaaaaacg ccgctcagta
    35041 gatccttgcg gatcggctgg cggcgttttg ctttttattc tgtctcaact ctggatgttt
    35101 catgaagcaa ctcaccattc tgggctcgac cggctcgatt ggttgcagca cgctggacgt
    35161 ggtgcgccat aatcccgaac acttccgcgt agttgcgctg gtggcaggca aaaatgtcgc
    35221 tcgcatggta gaacagtgcc tggaattctc tccccgctat gctgtaatgg acgatgaagc
    35281 gagtgcgaaa cttcttaaaa cgatgctaca gcaacagggt agccgcaccg aagtcttaag
    35341 tgggcaacaa gccgcttgcg atatggcagc gcttgaggat gttgatcagg tgatggcagc
    35401 cattgttggc gctgctgggc tgttacctac gcttgctgcg atccgcgcgg gtaaaaccat
    35461 tttgctggcc aataaagaat cactggttac ctgcggacgt ctgtttatgg acgccgtaaa
    35521 gcagagcaaa gcgcaattgt taccggtcga tagcgaacat aacgctattt ttcagagttt
    35581 accgcaacct atccagcata atctgggata cgctgacctt gagcaaaatg gcgtggtgtc
    35641 cattttactt accgggtctg gtggcccttt ccgtgagacg ccattgcgcg atttggcaac
    35701 aatgacgccc gatcaagcct gccgtcatcc gaactggtcg atgggacgta aaatttccgt
    35761 cgattcggct accatgatga acaaaggtct ggaatacatt gaagcgcgtt ggctgtttaa
    35821 cgccagcgcc agccagatgg aagtgctgat tcacccgcag tcagtgattc actcaatggt
    35881 gcgctaccag gacggcagtg ttctggcgca gctgggtgaa ccggatatgc gtacgccaat
    35941 tgctcacacc atggcatggc cgaatcgcgt gaactctggc gtgaagccgc tcgatttttg
    36001 caaactaagt gcgttgacat ttgccgcacc ggattatgat cgttatccat gtctgaaact
    36061 ggcgatggag gcgtttgaac aaggccaggc agcgacgaca gcattgaatg ccgcaaacga
    36121 aatcaccgtg gctgcttttc ttgcgcaaca aatccgcttt acggatatcg ctgcgttgaa
    36181 tttatccgta ctggaaaaaa tggatatgcg cgaaccacaa tgtgtggatg atgtgttatc
    36241 tgttgatgcg aacgcgcgtg aagtcgccag aaaagaggtg atgcgtctcg caagctgagg
    36301 ataatccggc tacagagagt cgcgctattt gttagcgtag ggcttcagtg atatagtctg
    36361 cgccatctga tcgtaagtag ttggctttat aaggtcaggt atgccgtggt tttacacggc
    36421 ttttttttgt ataggcttca gtattcctga gtaccgtaaa ccctgtcagg gaataagaaa
    36481 cgcgtgatgt tgtctgctac tcaaccactt agcgaaaaat tgccagcgca tggctgtcgt
    36541 catgttgcga tcattatgga cggcaatggt cgctgggcaa aaaagcaagg gaagattcgt
    36601 gcctttgggc ataaagccgg ggcaaaatcc gtccgccggg ctgtctcttt tgcggccaac
    36661 aacggtattg aggcgttaac gctgtatgcc tttagtagtg aaaactggaa ccgaccagcg
    36721 caggaagtca gtgcgttaat ggaactgttt gtgtgggcgc tagatagcga agtaaaaagt
    36781 ctgcaccgac ataacgtgcg tctgcgtatt attggcgata ccagtcgctt taactcgcgt
    36841 ttgcaagaac gtattcgtaa atctgaagcg ctaacagccg ggaataccgg tctgacgctg
    36901 aatattgcgg cgaactacgg tggacgttgg gatatagtcc agggagtcag gcaactggct
    36961 gaaaaggtgc agcaaggaaa cctgcaacca gatcagatag atgaagagat gctaaaccag
    37021 catgtctgta tgcatgaact ggcccctgta gatttagtaa ttaggactgg gggggagcat
    37081 cgcattagta actttttgct ttggcaaatt gcctatgccg aactttactt tacagatgtt
    37141 ctctggcccg atttcgatga acaagacttt gaaggggcgt taaatgcctt tgctaatcga
    37201 gagcgtcgtt tcggcggcac cgagcccggt gatgaaacag cctgatgggg gtcgcttttg
    37261 ctgaagtatc gcctgatatc tgcttttgtg ttaatacccg tcgtcatcgc ggcgttgttt
    37321 ctgttgccgc cggtggggtt cgccattgta acgctggtgg tctgtatgct ggcagcgtgg
    37381 gaatggggac agcttagcgg ttttaccact cgttcgcagc gagtatggtt ggcggtgtta
    37441 tgtgggttat tgttggcgct gatgcttttt ctgttgccgg aatatcaccg aaatattcat
    37501 caaccgctgg ttgaaatctc actttgggct tcgctgggtt ggtggattgt cgcgctattg
    37561 ctggtgctgt tttacccagg ttccgcagca atctggcgta actctaaaac attgcgcatt
    37621 atttttggcg tgctaaccat tgttcccttc ttctggggca tgctggcgtt acgggcctgg
    37681 cactacgacg agaatcatta cagtggcgca atatggctgc tctatgtcat gatcctggta
    37741 tggggcgctg actctggcgc atatatgttt ggcaaattgt ttggtaaaca taaactggca
    37801 ccgaaggttt ctccgggtaa aacctggcaa ggctttatcg gtggactagt gaccgcagcg
    37861 gtaatctcat ggggttatgg catgtgggtg aatctcgacg tcgctcccgt caccttactc
    37921 atttgctcta ttgtcgcagc gttagcctca gtgctcggcg atctgaccga aagtatgttt
    37981 aagcgtgaag caggaattaa ggacagcggt catttaattc caggacacgg tggtatttta
    38041 gatcgtattg atagcctgac ggctgcggta ccggtctttg cttgcttgtt gttactggta
    38101 ttcaggacgc tttaacggaa ggtaatatgc tgagttttct ctgggatttg gcttcgttca
    38161 tcgttgcact gggtgtactt atcaccgtgc atgaatttgg tcatttctgg gttgcccggc
    38221 gttgtggtgt tcgcgttgag cgtttctcaa tagggtttgg taaggcgctc tggcggcgaa
    38281 ctgataagct cggcaccgaa tatgttatcg ccctgatccc gttgggcggt tatgtcaaaa
    38341 tgctggatga gcgcgcagaa ccggtcgttc cggaactccg ccaccatgcc ttcaataata
    38401 aatctgtcgg ccaacgagcg gcgattattg ccgcaggtcc ggttgcaaac ttcatttttg
    38461 ctatctttgc ctactggctg gtttttatta ttggtgtgcc tggcgtacgt ccggtggttg
    38521 gtgaaatagc agccaattcg atagctgcgg aagcacaaat tgcaccaggt acggaactaa
    38581 aagccgtaga tggtatcgaa acgcctgatt gggatgccgt gcgtttgcag ttggtcgata
    38641 aaattggcga tgaaagcacc accattacgg tagcgccatt tggcagcgac caacggcggg
    38701 atgtaaagct cgatttacgt cactgggcgt ttgagcctga taaagaagat ccggtatctt
    38761 cgttggggat tcgtcctcgt gggccgcaaa ttgaacctgt actggaaaat gtgcagccaa
    38821 actcggcggc aagcaaggca ggtttgcaag caggcgacag gatcgttaaa gtcgatggtc
    38881 agcccttaac gcagtgggtg acctttgtga tgcttgtccg ggataacccg ggtaaatcct
    38941 tagcgttaga aatcgaaagg caggggagtc ccttgtcttt gacattaatc ccggagagta
    39001 aaccgggtaa gggtaaagcg attggttttg tcggtattga gccgaaagtc attcctttgc
    39061 cagatgagta taaagttgta cgccagtatg ggccgttcaa cgctatcgtc gaagccacgg
    39121 acaaaacgtg gcagttgatg aagctgacgg tcagtatgct gggaaaattg atcaccggtg
    39181 atgtgaaact gaacaacctc agtgggccga tctctatcgc caagggggct gggatgacag
    39241 cggaactcgg ggtagtttat tacctgccgt ttcttgcgct tattagcgtg aacttaggga
    39301 taattaacct gtttccgttg cccgtacttg acggggggca tctgctgttc cttgcgatcg
    39361 aaaagatcaa gggcggaccg gtatccgagc gggttcaaga cttttgttat cgcattggct
    39421 cgattctgct ggtgctgtta atggggcttg cacttttcaa tgatttctct cggttatgag
    39481 agttagttag gaagaacgca taataacgat ggcgatgaaa aagttgctca tagcgtcgct
    39541 gctgtttagc agcgccaccg tatacggtgc tgaagggttc gtagtgaaag atattcattt
    39601 cgaaggcctt cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg tgcgcacagg
    39661 cgacacggtt aatgatgaag atatcagtaa taccattcgc gctctgtttg ctaccggcaa
    39721 ctttgaggat gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg taaaagaacg
    39781 tccgaccatt gccagcatta ctttctccgg taacaaatcg gtgaaagatg acatgctgaa
    39841 gcaaaacctc gaggcttctg gtgtgcgtgt gggcgaatcc ctcgatcgca ccaccattgc
    39901 cgatatcgag aaaggtctgg aagacttcta ctacagcgtc ggtaaatata gcgccagcgt
    39961 taaagctgtc gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg tgttccagga
    40021 aggtgtgtca gctgaaatcc agcaaattaa cattgttggt aaccatgctt tcaccaccga
    40081 tgaactgatc tctcatttcc aactgcgtga cgaagtgccg tggtggaacg tggtaggcga
    40141 tcgtaaatac cagaaacaga aactggcagg cgaccttgaa accctgcgca gttactatct
    40201 ggatcgcggt tatgcccgtt tcaacatcga ttctacccag gtcagtctga cgccagataa
    40261 aaaaggtatt tacgtcacgg tgaacatcac cgaaggcgat cagtacaagc tttctggcgt
    40321 tgaagttagc ggcaatcttg ccgggcactc cgctgaaatt gagcaactga ctaagatcga
    40381 gccgggcgag ctgtataacg gcaccaaagt gaccaaaatg gaagatgaca ttaaaaagct
    40441 tcttggtcgc tatggttatg cctatccgcg cgtacagtcg atgcctgaaa ttaacgatgc
    40501 cgacaaaacc gttaaattac gcgtgaacgt tgatgcgggt aaccgtttct atgtgcgtaa
    40561 gatccgtttt gaaggtaacg atacctcgaa agatgctgtc ctgcgtcgcg aaatgcgtca
    40621 gatggaaggt gcatggctgg ggagcgatct ggtcgatcag ggtaaggagc gtctgaaccg
    40681 tctgggcttc tttgaaactg tcgataccga tacccaacgt gttccgggga gcccggatca
    40741 ggttgatgta gtttacaagg tgaaagaacg taacactggt agcttcaact tcgggattgg
    40801 ttacggtact gaaagtggcg tgagcttcca ggctggcgtg caacaggata actggttagg
    40861 tacaggttat gctgttggta tcaacgggac caaaaacgat taccagacct atgctgaact
    40921 gtcggtaacc aacccgtact tcaccgtaga tggtgtaagc ctcggtggtc gtctcttcta
    40981 taatgacttc caggcagatg acgccgacct gtccgactat accaacaaga gttatggtac
    41041 agacgtgacg ttgggcttcc cgatcaacga atataactcg ctgcgtgcag gtctgggtta
    41101 tgtacataac tccctgtcca acatgcagcc tcaggttgcg atgtggcgtt atctgtactc
    41161 tatgggtgaa catccgagca cctctgatca ggataacagc ttcaaaacgg acgacttcac
    41221 gttcaactat ggttggacct ataacaagct tgaccgtggt tacttcccga cagatggttc
    41281 acgtgtcaac ctgaccggta aagtgaccat tccgggatcg gataacgaat actacaaagt
    41341 gacgttagac acggcgactt atgtgccgat tgatgacgat cacaaatggg ttgttctggg
    41401 tcgtacccgc tggggttatg gtgatggttt aggcggcaaa gagatgccgt tctatgagaa
    41461 cttctatgcc ggtggttcca gcaccgtgcg tggcttccag tccaatacca ttggtccgaa
    41521 agcagtttac ttcccgcatc aggccagtaa ttatgatccg gactatgatt acgaatgtgc
    41581 gactcaggac ggcgcgaaag atctgtgtaa atcggatgat gctgtaggcg gtaacgccat
    41641 ggcggttgcc agcctcgagt tcatcacccc gacgccgttt attagcgata agtatgctaa
    41701 ctcggttcgt acttccttct tctgggatat gggtaccgtt tgggatacaa actgggattc
    41761 cagccaatat tctggatatc cggactatag tgatccaagc aatatccgca tgtctgcggg
    41821 tatcgcatta caatggatgt ccccattggg gccgttggtg ttctcctacg cccagccgtt
    41881 caaaaagtac gatggagaca aggcagaaca gttccagttt aacatcggta aaacatggta
    41941 agtgttctcc acaaaggaat gtagtggtag tgtagcgatg actttaggcg atcaatataa
    42001 gatcgccggg ccacgcaaag aactgcaccc tccggtgcaa atgggatggt aaggagttta
    42061 ttgtgaaaaa gtggttatta gctgcaggtc tcggtttagc actggcaact tctgctcagg
    42121 cggctgacaa aattgcaatc gtcaatatgg gcagcctgtt ccagcaggta gcgcagaaaa
    42181 ccggtgtttc taacacgctg gaaaacgagt tcaaaggccg tgccagcgaa ctgcagcgta
    42241 tggaaaccga tctgcaggct aaaatgaaaa agctgcagtc catgaaagcg ggcagcgatc
    42301 gcactaagct ggaaaaagac gtgatggctc agcgccagac ttttgctcag aaagcgcagg
    42361 cttttgagca ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg gttactcgta
    42421 tccagactgc tgtgaaatca gttgccaaca gccaggatat cgatctggtt gttgatgcaa
    42481 acgccgttgc ttacaacagc agcgatgtaa aagacatcac tgccgacgta ctgaaacagg
    42541 ttaaataagt aatgccttca attcgactgg ctgatttagc gcagcagttg gatgcagaac
    42601 tacacggtga tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct gcacaaacag
    42661 gtcacattac gttcatggtt aacccaaaat accgtgagca tttaggcttg tgccaggcgt
    42721 ccgcggttgt catgacccag gacgatcttc ctttcgcgaa aagtgccgcg ctggtagtga
    42781 agaatcccta cctgacttac gcgcgcatgg cgcaaatttt agataccacg ccgaagcccg
    42841 cgcagaacat tgcacccagt gcggtgattg acgagacagc gaagctgggt aacaacgtat
    42901 cgattggcgc taacgcggtg attgagtctg gcgttgaact gggcgataac gtgattatcg
    42961 gtgccggttg cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt ctctgggcga
    43021 acgtaaccat ttaccatgag atccagatcg gtcagaattg cctgatccag tccggaacag
    43081 tggtaggcgc agacggcttt ggttatgcca acgatcgtgg taactgggtg aagatcccac
    43141 agattggtcg ggtaattatt ggcgatcgcg tggagatcgg tgcctgcact actattgatc
    43201 gtggcgcgct ggatgacact gttattggca atggtgtgat cattgataac cagtgccaga
    43261 ttgcacataa cgtcgtgatt ggcgacaata cggcagttgc cggtggcgtc attatggcgg
    43321 gcagcctgaa aattggtcgt tactgcatga tcggcggagc cagcgtaatc aacgggcata
    43381 tggaaatatg cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt cccatcactg
    43441 aaccaggcgt ctattcctca ggcattccgc tgcaacccaa caaagtctgg cgcaaaaccg
    43501 ctgcactggt gatgaacatt gatgacatga gcaagcgtct gaaatcgctt gagcgcaagg
    43561 ttaatcaaca agactaacgt tccatctttt gttcgccaaa ctttacggcc tgtctcattc
    43621 ttacgattgc ggcaggccgt gttattattg tcgtttctta tattttgaca ggaagagtat
    43681 cttgactact aacactcata ctctgcagat tgaagagatt ttagaacttc tgccgcaccg
    43741 tttcccgttc ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt ttctgcgcgc
    43801 agtaaaaaat gtctctgtca atgagccatt cttccagggc catttccctg gaaaaccgat
    43861 tttcccgggt gtgctgattc tggaagcaat ggcacaggca acaggtattc tggcgtttaa
    43921 aagcgtagga aaactggaac cgggtgagct gtactacttc gccggtattg acgaagcgcg
    43981 ctttaagcgc ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt tcgaaaaaac
    44041 gcgccgcggc ctgacccgtt ttaaaggggt tgctctggtc gacggtaaag tagtttgcga
    44101 agcaacgatg atgtgtgctc gtagccggga ggcctgatac gtgattgata aatccgcctt
    44161 tgtgcatcca accgccattg tggaagaggg cgcgacgatt ggcgcgaacg ctcacattgg
    44221 tcctttttgt atcgttggac cccatgtcga aattggtgag ggtaccgtac tgaaatctca
    44281 cgttgtcgtg aatggtcata ctaaaattgg ccgcgataat gagatttatc agttcgcctc
    44341 catcggcgaa gttaaccagg atctgaaata tgctggcgaa ccgacccgcg tggaaatcgg
    44401 cgatcgtaac cgcattcgcg aaagcgtcac cgttcatcgt ggcacagtcc agggcggtgg
    44461 attgacgaag gtgggcagcg acaacttact gatgatcaat gcgcacattg cgcacgattg
    44521 tacggtaggt aaccgctgca tcctcgccaa caacgcaacg ctggcgggtc acgtatcggt
    44581 tgacgacttc gcgatcatcg gcggcatgac cgcagtccat cagttctgca tcattggtgc
    44641 gcatgtgatg gttggcggct gctccggtgt ggcgcaggac gtccctcctt atgtcattgc
    44701 gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc gccgcggatt
    44761 cagccgtgag gcgattaccg ctatccgcaa tgcgtataag ctgatttatc gtagcggtaa
    44821 aacgctcgat gaagtgaaac cggaaattgc tgaactggcg gaaacatatc cggaagtgaa
    44881 agcctttacc gatttctttg cacgctcaac gcgcggtctg attcgttaat gactgaacag
    44941 cgtccattaa cgattgccct ggtcgccgga gaaacctccg gcgatatcct gggggccggt
    45001 ttaatccgcg ctctgaaaga acgtgtgccc aacgcccgct ttgttggtgt tgccgggcca
    45061 cgaatgcagg ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc agtgatgggc
    45121 attgttgaag tgctcggtcg tctgcgtcgc ttactacata ttcgtgccga tctgacaaag
    45181 cgttttggcg aactgaagcc agatgttttt gttggtattg atgcgcctga cttcaatatt
    45241 actcttgaag gtaacctcaa aaagcagggt atcaaaacca ttcattacgt cagtccgtcc
    45301 gtctgggcgt ggcgacagaa acgcgttttc aaaataggca gagccaccga tctggtgctc
    45361 gcatttctgc ctttcgaaaa ggcgttttat gacaaataca acgtaccgtg ccgctttatc
    45421 ggtcatacca tggctgatgc catgccatta gatccagata aaaatggtgc ccgtgatgtg
    45481 ctggggatcc cttacgatgc ccactgtctg gcattgttgc cgggcagccg tggtgcagaa
    45541 gtcgaaatgc ttagtgccga tttcttgaaa acggcccagc ttttgcgcca gacatatccg
    45601 gatctcgaaa tcgtggtgcc gctggtgaat gccaaacgcc gcgagcagtt tgaacgcatc
    45661 aaagctgcag tcgcgccaga cctgtcggtt catttgctgg atggaatggg ccgtgaggcg
    45721 atggtcgcca gtgatgcggc gctactggcg tcgggtacgg cagcactgga gtgtatgctg
    45781 gctaaatgcc cgatggtagt gggatatcgc atgaagcctt tcaccttctg gctggcgaag
    45841 cgactggtga aaactgatta tgtctcgctg ccaaatctgc tggcgggcag agagttagtc
    45901 aaagagctat tgcaggaaga gtgtgagccg caaaaattgg ctgcggcgct gttaccgctg
    45961 ttggcgaacg ggaaaaccag ccatgcgatg cacgacacct tccgcgagct gcatcagcag
    46021 atccgctgca atgccgatga gcaggcggca caagccgttc tggagttagc acaatgatcg
    46081 aatttgtcta tccgcacacg cagctggttg cgggtgtgga tgaagtcgga cgcgggccgt
    46141 tagttggcgc ggtcgtcacc gctgcggtga tccttgaccc ggcgcgccca attgccgggc
    46201 tgaatgattc caaaaagctg agcgaaaaac gccgtctggc gctctgtgaa gagatcaaag
    46261 agaaagcgtt gagctggagt ctgggccgcg cggaacctca cgaaatcgac gaactgaata
    46321 ttttacatgc gaccatgctg gcgatgcagc gtgccgtcgc tggactgcat attgcgccgg
    46381 aatatgtgtt gattgatggt aaccgctgcc cgaaattacc gatgccttcg atggctgtgg
    46441 tgaaaggcga tagccgcgta ccggaaatca gtgccgcgtc tatcctggcg aaagtgacgc
    46501 gtgacgccga aatggcagcg ctggatattg ttttcccgca atatggtttt gcccaacaca
    46561 aagggtatcc aaccgctttt catctggaaa aactggctga acacggcgcg accgaacacc
    46621 atcggcgcag ctttgggcct gtcaaacgcg cactgggact tgcgtcctga ttcttgtgtc
    46681 gagattaagt aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac ctgcgggtgc
    46741 acagcgacta ctcgatgatc gatggcctgg ccaaaaccgc accgctggta aaaaaggcgg
    46801 cagcgttggg tatgccagca ctggcgatca ccgatttcac caacctttgc ggtctggtga
    46861 agttctacgg agcgggacat ggcgcaggga ttaagcccat cgtcggggcg gattttaacg
    46921 tccagtgcga cctgctgggt gatgagttaa ctcacctgac ggtgctggcg gcgaacaata
    46981 ccggctatca gaatctgacg ttgctgatct caaaagcgta tcagcgcggg tacggtaccg
    47041 ccgggccgat catcgatcgc gactggctta tcgaattaaa tgaagggttg atccttcttt
    47101 ccggcgggcg catgggcgac gttggacgca gtcttttgcg tggtaacagc gcgctggtag
    47161 atgagtgtgt cgcgttttat gaagaacact tcccggatcg ctattttctc gagctgatcc
    47221 gcaccggcag gccggatgaa gaaagctatc tgcacgcggc ggtggaactg gcggaagcgc
    47281 gcggtttgcc cgtcgtggcg actaacgatg tgcgctttat cgacagcagc gactttgacg
    47341 cacacgaaat ccgcgtcgcg atccacgacg gctttaccct cgacgatcct aaacgccctc
    47401 gtaactattc gccgcagcaa tatatgcgta gcgaagagga gatgtgtgag ctgtttgccg
    47461 acatccccga agcccttgcc aacaccgttg agatcgccaa acgctgtaac gtgaccgtgc
    47521 gccttggtga atacttcctg ccgcagttcc cgaccgggga catgagcacc gaagattatc
    47581 tggtcaagcg tgcaaaagag ggcctggaag agcgtctggc ctttttattc cctgacgagg
    47641 aagaacgtgt taagcgccgc ccggaatatg acgaacgtct ggatactgaa cttcaggtta
    47701 tcaaccagat gggcttcccg ggttacttcc tcatcgttat ggaatttatc cagtggtcga
    47761 aagataacgg tgtaccggta gggccaggcc gtggttctgg tgcagggtca ctggtggcct
    47821 acgcgttaaa aatcaccgat ctcgatccgc tggaatttga cctgctgttc gaacgtttcc
    47881 ttaacccgga acgtgtctcc atgcctgact tcgacgttga cttctgtatg gagaaacgcg
    47941 accaggttat cgagcacgta gcggacatgt acggtcgtga tgcggtatcg cagattatca
    48001 ccttcggtac gatggcggcg aaagcggtga tccgcgacgt aggccgcgtg ctggggcatc
    48061 cgtacggctt tgtcgaccgt atctcgaaac tgatcccgcc cgatccgggg atgacgctgg
    48121 cgaaagcgtt tgaagccgag ccgcagctgc cggaaatcta cgaagcggat gaagaagtta
    48181 aggcgctgat cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc ggtaagcacg
    48241 ccggtggggt ggttatcgca ccgaccaaaa ttaccgattt tgcgccgctt tactgcgatg
    48301 aagagggcaa acatccggtc acccagtttg ataaaagcga cgttgaatac gccggactgg
    48361 tgaagttcga cttccttggt ttgcgtacgc tcaccatcat caactgggcg ctggagatga
    48421 tcaacaagcg gcgggcgaag aatggcgagc cgcctctgga tatcgccgcg atcccgctgg
    48481 atgacaagaa aagcttcgac atgctgcaac gctcggaaac cacggcggta ttccagcttg
    48541 aatcgcgcgg catgaaagac ctgatcaagc gtctgcaacc tgactgcttc gaagatatga
    48601 tcgcactggt ggcacttttc cgccccggtc cgttgcaatc agggatggtg gataacttta
    48661 tcgaccgtaa acatggtcgc gaagagatct cctatccgga cgtgcagtgg cagcatgaaa
    48721 gcctgaaacc ggtactggag ccaacctacg gcatcatcct gtatcaggaa caggtcatgc
    48781 agattgccca ggtgctttct ggttataccc tcggtggcgc ggatatgctg cgtcgtgcga
    48841 tgggtaagaa aaagccggaa gagatggcta agcagcgttc tgtatttgct gaaggtgcag
    48901 aaaagaacgg aatcaacgcc gaactggcga tgaaaatctt cgacctggtg gagaaattcg
    48961 cgggttacgg atttaacaaa tcgcactctg cggcctatgc tttggtgtca tatcaaacgt
    49021 tatggctgaa agcgcactat ccggcggagt ttatggcggc ggtaatgacc gccgatatgg
    49081 acaacaccga gaaggtggtg ggcctggtgg atgagtgctg gcggatgggg ctgaaaatcc
    49141 tgccaccaga tataaactcc ggtctttacc atttccacgt caacgacgac ggcgaaattg
    49201 tgtatggtat tggcgcgatc aaaggggtag gtgaaggccc gattgaggcc atcatcgaag
    49261 cccgtaataa aggcggctac ttccgcgaac tgtttgatct ctgcgcccgt accgacacca
    49321 aaaagttaaa ccggcgggtg ctggaaaaac tgatcatgtc cggggcgttt gaccgtcttg
    49381 ggccacaccg cgcggcgctg atgaactcgc tgggcgatgc gttaaaagcg gcagatcaac
    49441 acgcgaaagc ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc gaagagccgg
    49501 aacaaattga acaatcctac gccagctgcc aaccgtggcc ggagcaggtg gtgttagatg
    49561 gggaacgtga aacgttaggt ctgtacctca cgggacaccc tatcaaccag tatttaaaag
    49621 agattgagcg ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca gaacgtggta
    49681 aagtcatcac ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc aagcgcggca
    49741 atcgtatcgg tatctgcacg ctggatgacc gttccgggcg gctggaagtg atgttgttta
    49801 ctgacgccct ggataaatac cagcaattgc tggaaaaaga ccgcatactt atcgtcagcg
    49861 gacaggtcag ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc gaagtgatgg
    49921 atattgacga agcccgggaa aaatatgctc gcgggcttgc tatctcgctg acggacaggc
    49981 aaattgatga ccagctttta aaccgactcc gtcagtctct ggaaccccac cgctctggga
    50041 caattccagt acatctctac tatcagaggg cggatgcacg cgcgcgattg cgttttggcg
    50101 cgacgtggcg tgtctctccg agcgatcgtt tattaaacga tctccgtggc ctcattggtt
    50161 cggagcaggt ggaactggag tttgactaat acaggaatac tatgagtctg aatttccttg
    50221 attttgaaca gccgattgca gagctggaag cgaaaatcga ttctctgacc gcggttagcc
    50281 gtcaggatga gaaactggat attaacatcg atgaagaagt gcatcgtttg cgtgaaaaaa
    50341 gcgtagaact gacgcgtaaa atcttcgccg atctcggtgc atggcagatt gcgcaactgg
    50401 cacgccatcc acagcgtcct tataccctgg attacgttcg cctggcattt gatgaatttg
    50461 acgaactggc tggcgaccgc gcgtatgcag acgataaagc tatcgtcggt ggtatcgccc
    50521 gtctcgatgg tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa accaaagaaa
    50581 aaattcgccg taactttggt atgccagcgc cagaaggtta ccgcaaagca ctgcgtctga
    50641 tgcaaatggc tgaacgtttt aagatgccta tcatcacctt tatcgacacc ccgggggctt
    50701 atccgggcgt gggcgcagaa gaacgtggtc agtctgaagc cattgcacgc aacctgcgtg
    50761 aaatgtctcg cctcggcgta ccggtagttt gtacggttat cggtgaaggt ggttctggtg
    50821 gtgcgctggc gattggcgtg ggcgataaag tgaatatgct gcaatacagc acctattccg
    50881 ttatctcgcc ggaaggttgt gcgtccattc tgtggaagag cgctgacaaa gcgccgctgg
    50941 cggctgaagc gatgggtatc atcgctccgc gtctgaaaga gctgaaactg atcgactcca
    51001 tcatcccgga accgctgggc ggtgctcacc gtaacccgga agcgatggcg gcgtctctga
    51061 aagcgcagct gctggcggat ctggccgatc tcgacgtgtt aagcactgaa gatttaaaaa
    51121 atcgtcgtta tcagcgcctg atgagctacg gctacgcgta atcggcaaat gttctgaaaa
    51181 gggtcacttc ggtggccctt ttttatcgcc acggtttgag caggctatga ttaaggaagg
    51241 attttccagg aggaacacat gaacatcatt gccattatgg gaccgcatgg cgtcttttat
    51301 aaagatgagc ccatcaaaga actggagtcg gcgctggtgg cgcaaggctt tcagattatc
    51361 tggccacaaa acagcgttga tttgctgaag tttatcgaac ataacccacg aatttgcggc
    51421 gtgatttttg actgggatga gtacagtctc gatttatgta gcgatatcaa tcaacttaat
    51481 gaatatctcc cgctttatgc cttcatcaac acccactcga cgatggatgt cagcgtgcag
    51541 gatatgcgga tggcgctctg gttttttgaa tatgcgctgg ggcaggcgga agatatcgcc
    51601 attcgtatgc gtcagtacac caacgaatat cttgataaca ttacgccgcc gttcacgaaa
    51661 gccttgttta cctacgtcaa agagcggaag tacacctttt gtacgccggg gcatatgggc
    51721 ggcaccgcat atcaaaaaag cccggttggc tgtctgtttt atgatttttt cggcgggaat
    51781 accctcaagg cggacgtctc tatttcggtc accgagcttg gttcgttgct cgaccacacc
    51841 gggccacacc tggaagcgga agagtacatc gcgcggacgt ttggcgcgga acagagttat
    51901 atcgttacca acggaacatc gacgtcgaac aaaattgtgg gtatgtacgc cgcgccatcc
    51961 ggcagtacgc tgttgatcga ccgcaattgt cataaatcgc tggcgcatct gttgatgatg
    52021 aacgatgtag tgccagtctg gctgaaaccg acgcgtaatg cgttggggat tctgggtggg
    52081 atcccgcgcg gtgaatttac tcgcgacagc atcgaagaga aagtcgctgc caccacgcag
    52141 gcacaatggc cggttcatgc ggtgatcacc aactccacct atgatggctt gctctacaac
    52201 accgactgga tcaaacagac gctggatgtc ccgtcgattc acttcgattc tgcctgggtg
    52261 ccgtacaccc attttcatcc gatctaccag ggtaaaagtg gtatgagcgg cgagcgtgtt
    52321 gcgggaaaag tgatcttcga aacgcaatct acccacaaaa tgctggcggc gttatcgcag
    52381 gcttcgctga tccacattaa aggcgagtat gacgaggagg cgtttaacga agcctttatg
    52441 atgcatacca ccacctcgcc cagttatccc attgttgctt cggttgagac ggcggcggcg
    52501 atgctgcgtg gtaatcctgg caaacggctg attaaccgtt cagtagaacg agctctgcat
    52561 tttcgcaaag aggtccagcg gctgcgggaa gagtctgacg gctggttttt tgatatctgg
    52621 caaccgccgc aggtggatga agccgaatgc tggcccgttg cgcctggcga acagtggcac
    52681 ggctttagcg atgcggatgc caatcacatg tttctcgatc cggttaaagt cactattttg
    52741 acaccgggga tggacgagca gggcaatatg agcgaggagg ggatcccggc ggcgctggtg
    52801 gcaaaattcc tcgacgaacg tgggagcgta gtagagaaaa ccggccctta taacctgctg
    52861 tttctcttta gtattggcat cgataaaacc aaagcaatgg gattattgcg tgggttgacg
    52921 gaatttaagc gctcttacga tctcaacctg aggatcaaaa atatgctacc cgacctctat
    52981 gcagaagatc ccgatttcta ccgcaatatg cgtattcagg atctggcgca agggatccat
    53041 aagctgattc gtaaacacga tcttcccggt ttgatgttgc gggcatttga taccttgccg
    53101 gagatgatca tgacgccaca tcaggcatgg cagagacaga ttaaaggcga agtagaaacc
    53161 attgcgctgg aacaactggt cggtagggtg tcggcaaata tgatcctgcc ttatccaccc
    53221 ggcgtaccgc tgctgatgcc gggagaaatg cttaccgaag agagccgcac ggtactcgat
    53281 tttctactga tgctttgttc cgtagggcaa cattaccccg gttttgaaac ggatattcac
    53341 ggcgcgaaac aggacgaaga cggtgtttac cgcgtacgag tcctaaaaat ggcaggataa
    53401 cttgccagag cggcttccgg gcgagtaacg tgctgttaac aaataaagga gacgttatgc
    53461 tgggtttaaa acaggttcac catattgcga ttattgcgac ggattatgcg atgagcaaag
    53521 ctttctactg cgatattctt ggtttcacgc tgcaaagcga agtctatcgc gaagcgcgcg
    53581 actcgtggaa aggggatttg gcgcttaatg ggcaatatgt gattgagctt ttctcatttc
    53641 cgttcccgcc ggagcgcccc agccgaccgg aagcttgcgg cctgcgtcat ctggctttta
    53701 gcgttgatga tatcgatgcg gcagtggcgc accttgaaag ccataacgtg aagtgtgaag
    53761 ccatccgtgt cgatccctac acgcaaaaac gcttcacgtt ctttaacgat ccggacgggc
    53821 tgccgttgga actgtatgag cagtaaggct tgtcatcgcc gcatttgccc ggtaacgtgc
    53881 ccggcattgc tactgtaaaa tcgcaccatc atgacactca cgctcaatag acaacttctc
    53941 tcctcacgcc agattctggt ggcctttagc ggcggccttg attctactgt cttactgcat
    54001 caactggtgc agtggcggac ggaaaatccg ggcgtcactc tgcgcgctat ccatgtgcat
    54061 cacggtttaa gcgccaatgc tgatgcctgg gtgaaacatt gtgaaaacat ctgccaacag
    54121 tggcaggtgc cgctggtggt cgaacgtgtg cagcttgcgc aagaggggct aggtattgag
    54181 gcccaggcgc ggcaggcacg ttatcaggca tttgcccgca ccttgttgcc cggtgaagtg
    54241 ctggttacag cgcaacatct cgacgatcaa tgtgaaacct ttctgctggc gctaaaacgc
    54301 ggtagcggcc ctgccgggct ttcggctatg gcggaagttt cggagtttgc cggaacacgg
    54361 cttatccgcc cgttgcttgc ccgcacgcgg ggggaactgg agcagtgggc gttagcgcat
    54421 ggtttacgct ggattgaaga cgaaagtaat caggatgact catacgatcg taactttctg
    54481 cgcctgcgcg tagtgccatt attgcagcag cgttggccgc attttgccga agcaacagcc
    54541 cgcagcgccg cactttgcgc tgaacaagag agcctgttag atgaactgct ggcggatgat
    54601 ttagcgcact gccaaacgca acagggaacg ttaaagattg cgccaatggt ggcaatgagc
    54661 gatgcccgtc gcgcggcgat tatccgccgc tggctggcag ggcagaatgc accgatgcct
    54721 tcccgcgacg cgttggtgag gatctggcag gaagtggcgc tggcgcgaga agatgcttcc
    54781 ccctgtttac gtttgggtgc gtttgaaatc cgccgctatc agtcgcaact gtggtggatt
    54841 aaatccgtca ctgggcaaag cgaaaccatt ctgccgtggc agacgtggct acaaccgctg
    54901 gaattaccgg cggggctggg aagtgtacag cttactgcgg gaggcgatat tcgccctccg
    54961 cgtgcagacg aagcggtcag cgtgcgtttc aaagcgccag gattgctgca tattgtcggg
    55021 cgcaacggcg gacgtaagct gaagaaaatc tggcaagagt taggcgttcc gccgtggcta
    55081 cgtgacacca cgccactgct gttttatggc gaaacgctga ttgcggcggc aggggtattt
    55141 gtgacgcaag aaggtgtggc tgtaggtgag aatggcgtca gttttgtctg gcagaaaacg
    55201 cttagtcaag tgaaagccgg ataagacgca tcaaacgtcg catccggcga aagtcaatca
    55261 ggactcgctc accaccaccg taccgatttc cgggtggcta aagctggtaa ttttatccag
    55321 acgcagctcg cgggttgcac cagcggcctc gacgaccaga tactccacat ttttgcggga
    55381 gactaaatcg ctggccttcg cctgcaattt ttcgccatct ttcagctcaa gtgtcagcat
    55441 taaatgatgc tggcaggcga gctcaagatt atcgtaatca tcacaattga ttggttgata
    55501 cgtatcattc attgacataa tcgctcacca gtaagtttgc cgcagcgtat gctgcttttt
    55561 ccctgacagc ctcagaaagg gcgtcgtcgg cagccatttc attcagcact ttcaaaacgc
    55621 agcccagcgc gtccggaacg tatcctaagt ctccgctggc gatttccgcg taccgcttgc
    55681 gtattaactc acaatatttt tccacatgcc ctcctgtcaa cactctgact taaccgtgga
    55741 tgcaagtcta agcctacgaa gataaactct gtttcgcaag gtgactatac cacactcatt
    55801 tctgcaatat cagcgccgca actgcacgta ttccgttaca atggcctcct gattcgaaag
    55861 gagttttctt atggcgctta aagcgacaat ttataaagcg acggttaatg tggccgatct
    55921 cgaccgcaac cagtttctcg atgcctctct gacgctggcg cgccatcctt cagaaaccca
    55981 ggagcgcatg atgctgcgcc tgctggcgtg gctaaaatat gccgatgaac gtctgcaatt
    56041 tacccgtggt ttgtgtgccg atgatgagcc ggaagcgtgg ctgcgtaacg atcacctggg
    56101 cattgatttg tggattgaac tggggctgcc ggatgagcgg cggattaaga aagcctgcac
    56161 ccaggccgca gaagtggcgc tgtttgccta taatagtcgg gcggcgcaaa tctggtggca
    56221 gcaaaatcag agcaaatgtg cgcagtttgc caatctttcc atctggtatc tggacgatga
    56281 acaactggcg aaagtaagcg cctttgccga tcgtaccatg acgctgcagg caacgattca
    56341 ggatggcgtg atctggttat cggatgataa gaataatctg gaagtgaact taaccgcctg
    56401 gcaacaacct tcatgattgt gatttcccga catgttgcta tccccgatgg tgagcttgag
    56461 atcaccgcca ttcgtgcgca gggcgcgggc gggcagcatg ttaataagac ctcaacggct
    56521 attcatctgc gttttgacat tcgggcgtcc agcctgccag agtattacaa agaacgtctg
    56581 ctcgccgcca gccatcactt gatcagcagt gatggggtga ttgtcattaa ggcacaggaa
    56641 taccgcaatc aggagctcaa ccgcgaagcg gcgctggccc ggctggtggc agtgattaaa
    56701 gatttaacaa cagaacaaaa agcccgacga cccacgcggc ccacccgcgc atcaaaagag
    56761 cgcaggctgg catcgaaagc acaaaaatca agcgtgaagg cgatgcgcgg caaagtgcgc
    56821 agcggtcggg aataaaaaga aggaatggat ggtgaaaaaa gcgatagtga cagcgatggc
    56881 tgtaatcagc ctctttactc tgatgggatg taataatcgg gccgaagtcg atacgctttc
    56941 tccggcacag gctgccgaac tgaaaccgat gccgcaaagt tggcgcggcg tgctgccgtg
    57001 tgctgattgc gaaggaatcg aaacctctct gttcctcgaa aaagacggaa cctgggtgat
    57061 gaatgagcgt tatctggggg ctcgtgaaga accttcctcc ttcgcttcct acggtacatg
    57121 ggcgcgaacc gctgacaagc tggtattaac cgatagcaaa ggtgaaaagt catattatcg
    57181 cgcgaaagga gatgcgctgg agatgctcga tcgtgaaggt aatccgattg aatcgcagtt
    57241 caactatacg ctggaaccgg cacaatccag cttacccatg acaccgatga ccctgcgggg
    57301 catgtatttt tatatggctg atgcggcgac cttcactgat tgcgcgaccg gaaaacgttt
    57361 catggtggcg aataacgcag agctggagcg tggctacctg gctgcgcgcg gtaacagtga
    57421 aaaaccggta ttactgtcag tagaaggtca ctttacgctt gaggctaatc cggataccgg
    57481 tgcgccgacc aaagtattag cgcccgatac ggcaggtaaa ttttacccaa accaggattg
    57541 cagtagtttg gggctgtaac ccgtcttgag acagaaacaa atgaaatatt gcctcatgcg
    57601 ctgcgcttat cagtcctaaa gcatacaatg caatattttg aattagcaca attttgtagg
    57661 ccggataagg cgttcacgcc gcatccggca ttttgcgcac gaggctggct tttatcactg
    57721 agtcagccca acaaagcggc ctgccttgat ataaatccca ggcttcatgt gcccaacata
    57781 ctgtaactgc gtttctggct taaacgcaga gatatcgcca gtacgcatat gcatcagatc
    57841 ggcagggcta atccagcctg attgcgccag cgtaagcgga tgcccggctt tggcaaatgc
    57901 atcagtgaca aattccgaac aaaaccacga ctttttgtct ccttcgccca cactgcttaa
    57961 ctgcgctttc gccaggccgc tgacgcactg ttgacgaaaa tcctcggaga acggatttag
    58021 tgagcacatc tggcgagtca ccataaatgg aataaattcg acaatgccgc gatagttgta
    58081 accgctatct ttaattttgt tggcgaacgc ggtgatttct gtggcttgtt gcggggtaag
    58141 atccgggact cgtaagacga aaagcttatc actatgcttt atggcttttt taagggaaac
    58201 aatctggacg ccagcgcccg tcgcttcggc aacgttatta tctcccaaat agattgcaac
    58261 gtgactcaca gaggaagtgc tgaatacgcg gataccaaat gaggttaccc caaggcttga
    58321 ggagaaaagc aaatcgccag gtttgagatc tggttccgtt atttctttta ttgattgttc
    58381 ggtgaaagag ctttgatgct ggaatttaac agcccatgtt ttcgcctcgg catttaccac
    58441 tgtggctgat gagtctggct ggctgatatc aacggtacag gccgaaagta aaagaaagca
    58501 ggggagaagc aggcggcagt acgcctttgg tttatccatt ttatacaatc catgtaaaaa
    58561 agggccccga aattcaggac cctttctggc atcagccttt aatctgtttc accagataat
    58621 cgacgatgtc accagtctta attaactgtt tctcgccgtt acgacgatat ttatattcga
    58681 tatcatcgtt gtcgaggtta cggtcgccca gcacaatagt gtgcggaata ccgatcagtt
    58741 ccatatcagc aaacatcacg cccggacgct ctttgcggtc atccagcagc acttcgatac
    58801 cttgtgcgcg cagttcgctg tacagtttct cagcaagttc ctgtacgcgg aaggatttgt
    58861 gcatgttcat tggcagaatc gccacctgga acggcgcgat agcgtcaggc catacgatgc
    58921 cgcgttcgtc gtagttctgc tcaatcgccg ctgctaccac acgcgttacg ccgataccgt
    58981 agcaacccat cgtcaggatt tggttacggc catcttcacc ctgtacggag gctttcagtg
    59041 cttcggagta cttggtaccc agctggaaga tgtgaccgac ttcgatacca cgtttgatca
    59101 gcagcgtacc ctgaccatcc gggcttggat cgccagccac cacgttgcgg atatcagcaa
    59161 tttccggggt agcgacatcg cgatcccagt tgataccgaa gtagtgttta ccatcgatgt
    59221 tagcaccagc agcgaaatca ctcatcgccg caacggtacg gtcaatcacc accggaatcg
    59281 gcatgtttac cggacccagt gaacccggac cggctttaac cacggcacga atttcttctt
    59341 cggtcgcaaa agtcagcggg ctggcaacct gcggcagttt ttctgcttta acttcgttca
    59401 gctcgtggtc accgcgcacc agcagcgcaa ccagcgggaa gctactgcct tcaacggctt
    59461 taaccagcag agtcttaacc gttttctcaa tcggcagatt gaactgttca accagttccg
    59521 cgatggtttt cgcgttcggc gtatcaacca gcgtcatttc ctgggtagca gcagcgcgcg
    59581 gttctttcgg cgcgatagct tctgccagtt caatgttcgc tgcatagtca gaagtatcgg
    59641 agaagaccac atcgtcttca ccgctctgcg ccagcacctg aaattcgtga gaggcgctac
    59701 cgccgataga accggtatcg gcctgtacgg cgcggaaatc cagccccatg cggctgaaga
    59761 ttttgctgta ggccgcatac attgcatcgt aggtttcctg cagggattcc tgagaagtat
    59821 ggaaagagta agcatctttc atcaggaatt cgcgggaacg catgacgcca aaacgcggac
    59881 gcacttcgtc gcggaacttg gtctggatct gatagaagtt cagcggcagc tgtttgtaag
    59941 agctcagctc gttacgaatc aggtctgtaa tcacttcttc atgagttggg ccgagtacga
    60001 acggacggtc accacggtca gcaatacgca gcaattccgg gccatactgt tcccaacgac
    60061 cactctcttg ccacagttca gatggctgaa ccaccggcat taacacctcg atcgcaccgg
    60121 cgttgttcat ctcttcacgc acgatgtttt cgactttttt cagaacgcgc acgccggtcg
    60181 gcagccaggt atataacccg gaggccagct tgcggatcat cccggcgcgc agcatcagct
    60241 gatggctgat cacctcggcg tcggcaggtg tctccttgag agtggagagc aggtattggc
    60301 tagtacgcat gttgttacgg ttccagttgg aaggtagaac aggctcaagg cgagcctggg
    60361 acaaaaaaag tgatttagtt taccagtgca aaagaaatgt caaaagagaa gggcgtgaat
    60421 ttaacgcggt tccagcgcaa agacttcaaa acctgcgtcg gtgacgcgcc agcgaacgtt
    60481 aaaatcatgc agccagacgg cataggtttt gcccgtttcc tcacctttac gataggccgg
    60541 gcgcgggtcc tgcgccagta cttcgcggat aaacagcgtt aactgcggat aacgcttctt
    60601 cagcgtcaaa agctgctttt cgacctctgc ggtaaaacac accgccattt ctgccgctgg
    60661 cgcactttgc gcatagctgg cactggcatc gggaagcgat tcagcaaagg ggagatacgg
    60721 tttgatatcc actaccggcg taccatcgac cagatccaga ctgccgagct tcagaatcac
    60781 gctatcttta tggcaaacaa cctctttcag ctctaccagc gacataccaa tcgggttagg
    60841 gcgaaaagtg gagcgcgtag cgaaaacacc cattctggca ttaccgccga gacgaggcgg
    60901 acgcacagtc ggacgccagc cgccttccat cgtttgatga aagacgaaaa ggatccataa
    60961 atggctgaat gcttccaggc cgcgaacggc gtcggcctgg ttgtagggag caatgagatg
    61021 tagttcgccg ttggcgcttt ttaccagacc tggctggcgc ggaacggcga atttttcttt
    61081 atagggcgag cgaataacgc ctatttgctc gaactggaaa ctgctcattt cgccgtgatg
    61141 ttaagcgcag aaccgataca tacagcctga cgatagcaac ctggcgtacc gctggtgact
    61201 tcgcagctat gcagtaatac cgcattggct ttcattttcg aggcgttgat ttgcatccgc
    61261 ttacgtgcgg ttggaatgct cggcggagag tcctgattag aggcctggca agagtcgcca
    61321 ctgacttcac cgagatcgcg gaacggtttg ccgactaatt cttctgcatt ggtataaatt
    61381 cggaccgacg tggcgcgcgg cgctttcggt tttgcaggct ccgctttcgg ctggggtgca
    61441 gtgctttgaa cgggttcgac aggggatctg cttaacatgg aacagccgct tagcatgagt
    61501 gctactaaac agatcggtaa agcacgcata gtatttcctc aatgtatgat caaaacgtca
    61561 atattgaatc aggcgcttgt aaaaatgaca agacgggcaa gcgcccgtcc tgaatgatat
    61621 tacaaattgt ggaaacagcc taaatattac cagcctttaa cagcgccgcc gttaaacact
    61681 ttgtttgctg cttcgtaaac ttcgtcagac tgataagcct gaacgaattt cttcacgttt
    61741 tcggcgtctt tgttgtcttc acgcgttacg atcaggttta cgtacgggga ctctttatct
    61801 tcgacaaaga taccgtcttt cgctggagtc aggccaatct ggctggcata ggtggtattg
    61861 ataactgcca gagcgatttg cgcgtcgtcc agagagcgcg gcagctgcgg tgcttccagt
    61921 tcaacaattt tcagattttt tgggttctca acaacatcaa gaacggtcgg cagcaggcca
    61981 acgccatctt tcagtttgat caagcccact ttttgcagca gcagcagtga acgaccaagg
    62041 ttagttgggt cgtttggcac ggcaacctgc gaaccatcct gcagttcatc cagtgatttg
    62101 attttcttgg agtaaccagc aatcggataa acaaaagtgt tgcctactgc gaccagtttg
    62161 tagccacgat ctttcagttg ctgatcaagg tacggtttat gctggaaggc gttggcgtcg
    62221 atatcgcctt tgctcaatgc ttcgtttggc agaacatagt cgttgaaggt gaccagctca
    62281 acgtccaggc catatttgtc tttcgcgact ttctgcgcga cttctgcaac ctgctgttcg
    62341 gcaccaacaa tcacgccgac tttaatgtgg tttggatctt tttcatcctg accgcagcct
    62401 accagtgcca gtgatccaat cagggctccc actgccgcaa aggttttgaa tttgaacgcc
    62461 ataccttatt ccttcttcaa ttatttatgt tgtgttgaac gttacttgcg agtgacagcc
    62521 cggacgatgc ggtcgcctgc gaactgaatt aaataaacca gaatgaccag caataccagt
    62581 accgtattca tcaccgtcgc gttgtagccg atgtagccat actgatagcc aatctgacct
    62641 aaaccaccgg caccgactgc accacccatc gcggaataac caaccagggt aatcagggta
    62701 atagttgccg cattcaccag acccggcaac gcttccggta acagcacttt acggacgatc
    62761 tgcattggcg tggcacccat tgcgcgggaa gcttcaatta acccggttgg gatctccaga
    62821 agagcgttct cgaccatacg ggcaataaac ggtgctgcac caacggttaa cggaacaatc
    62881 gctgcctgca atccaatcga tgtaccgaca ataacgcggg taaacggaat catccatacc
    62941 agcaagataa tgaacgggat ggaacggaaa atgttcacaa tcgcagaaat ggtacgatac
    63001 aacttcgcgt tagcaataat ttgccccgga cgcgtgacat aaagcagaac gccaaccggc
    63061 agaccaatca caaagccaaa aaaaccggat acgaaggtca ttgccagcgt ttcccatacg
    63121 ccacgaacca gcagccacat catcggctca gacataaccc agtacctcta cttttacatg
    63181 gtgttcctgc agccaggcaa tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt
    63241 cagcataatg ccgaacttaa cgccaccggc gtaatccatc tgcgcgctaa taatgttgtt
    63301 gttgacgttg aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg attgaccggt
    63361 aaactccaga cgcagcatcg gcacgcagtc agcgaatggc tccgcttgca gacgttcctg
    63421 gtaatcttcc gggatatcca gatgcagggt cgactgaata aacttctgcg ccagcggcgt
    63481 tttcggatgc gagaacactt cacttaccgt gtcctgctcg atcagttcgc cattgctgat
    63541 gaccgccacg caatcacaaa tgcgcttcac aacgtccatt tcgtgagtga tcaacagaat
    63601 cgtcaaaccc agacggcggt tgatgtcttt cagcagttcg agaatagaac gtgtcgttgc
    63661 cgggtccagc gcgctggtgg cttcatcaca cagcaatact ttgggattgc tggctaatgc
    63721 acgggcaatc gccacacgtt gtttctgccc accggaaaga ttcgacgggt agctatcatg
    63781 cttatcgcca agaccaacca atgacagcaa ttccgtcacg cgacgtttga tctcgtcttt
    63841 cggtgtgttg tccagctcca gcggcagagc cacgttgcca aaaacagtac gcgaagagag
    63901 caggttaaaa tgctggaaaa tcataccaat ctggcgacga gctttggtca actcggattc
    63961 tgacagcgtg gtcagttcct ggccatcgac cagcacgcta ccctcggttg ggcgctccag
    64021 caggtttaca caacgtataa gcgtactctt acccgcgcct gaggcaccga taacgccata
    64081 aatttgtcca gctggcacat gcaggctgac gttgttcaac gcctggatgg tgcgggtgcc
    64141 ctggtggaac actttggtga tattcgaaag ttttatcatt gattatttat tatcgtcatt
    64201 aagttagtcg tggcatttcg aatgcctgga acgggcaacg ccgtcaatga aatggatgtt
    64261 aaggcatcca gacgtctaaa tcaatcaggt ttatgcgaag agcactttct tgcaggtcga
    64321 aacatgcgat actagcgtca catgccttat taaggagcta taaaaggtgg cgaagagcgt
    64381 acccgcaatt tttcttgacc gtgatggcac cattaatgtc gatcacggct atgtccatga
    64441 gatcgacaac tttgaattta tcgacggtgt tattgacgcc atgcgcgagc taaaaaaaat
    64501 gggctttgcg ctggtggtag taaccaacca gtctggcatt gctcgcggta aatttactga
    64561 agcacagttt gaaacgctga ccgagtggat ggactggtcg ctggcggacc gcgatgtcga
    64621 tctggatggt atctattatt gcccgcatca tccgcagggt agtgttgaag agtttcgcca
    64681 ggtctgcgac tgccgcaaac cacatccggg gatgtttttg tcagcacgcg attatttgca
    64741 tattgatatg gccgcttctt atatggtggg cgataaatta gaagatatgc aggcagcggc
    64801 tgcggcgagc gtgggaacaa aagtgctggt gcgtacgggt aaacctatta cgcctgaagc
    64861 agaaaacgcg gcggattggg tgttaaatag cctggcagac ctgccgcaag cgataaaaaa
    64921 gcagcaaaaa ccggcgtaat gattaaaaga tgagctgttg aaataaaaat gcatttttcc
    64981 gcttgtcttc ctgagac
//
LOCUS       DADTFG010000006        62724 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.006, whole genome shotgun sequence.
ACCESSION   DADTFG010000006 DADTFG010000000
VERSION     DADTFG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.006"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(106..927)
                     /gene="bacA"
                     /locus_tag="J1138_000408"
     CDS             complement(106..927)
                     /gene="bacA"
                     /locus_tag="J1138_000408"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBA8452256.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(1018..1386)
                     /gene="folB"
                     /locus_tag="J1138_000409"
     CDS             complement(1018..1386)
                     /gene="folB"
                     /locus_tag="J1138_000409"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBA8452257.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1491..2108
                     /gene="plsY"
                     /locus_tag="J1138_000410"
     CDS             1491..2108
                     /gene="plsY"
                     /locus_tag="J1138_000410"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBA8452258.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2121..3053)
                     /gene="ttdR"
                     /locus_tag="J1138_000411"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(2121..3053)
                     /gene="ttdR"
                     /locus_tag="J1138_000411"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            3260..4171
                     /gene="ttdA"
                     /locus_tag="J1138_000412"
     CDS             3260..4171
                     /gene="ttdA"
                     /locus_tag="J1138_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBA8452259.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLRVHADLTFENLSHTRSAL"
     gene            4168..4773
                     /gene="ttdB"
                     /locus_tag="J1138_000413"
     CDS             4168..4773
                     /gene="ttdB"
                     /locus_tag="J1138_000413"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBA8452260.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4821..6284
                     /locus_tag="J1138_000414"
     CDS             4821..6284
                     /locus_tag="J1138_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBA8452261.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASNATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPILKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIICWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6327..7340)
                     /gene="tsaD"
                     /locus_tag="J1138_000415"
     CDS             complement(6327..7340)
                     /gene="tsaD"
                     /locus_tag="J1138_000415"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBA8452262.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     GVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7578..7793
                     /gene="rpsU"
                     /locus_tag="J1138_000416"
     CDS             7578..7793
                     /gene="rpsU"
                     /locus_tag="J1138_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBA8452263.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7904..9649
                     /gene="dnaG"
                     /locus_tag="J1138_000417"
     CDS             7904..9649
                     /gene="dnaG"
                     /locus_tag="J1138_000417"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBA8452264.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMERAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            9844..11685
                     /gene="rpoD"
                     /locus_tag="J1138_000418"
     CDS             9844..11685
                     /gene="rpoD"
                     /locus_tag="J1138_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBA8452265.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(11763..12269)
                     /gene="mug"
                     /locus_tag="J1138_000419"
     CDS             complement(11763..12269)
                     /gene="mug"
                     /locus_tag="J1138_000419"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBA8452266.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            12394..12469
                     /locus_tag="J1138_000420"
     tRNA            12394..12469
                     /locus_tag="J1138_000420"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12427..12429,aa:Ile,seq:cat)
     gene            complement(12523..13287)
                     /gene="nfeF"
                     /locus_tag="J1138_000421"
     CDS             complement(12523..13287)
                     /gene="nfeF"
                     /locus_tag="J1138_000421"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBA8452267.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPGTHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRHRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            13575..14198
                     /locus_tag="J1138_000422"
     CDS             13575..14198
                     /locus_tag="J1138_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBA8452268.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(14305..15603)
                     /locus_tag="J1138_000423"
     CDS             complement(14305..15603)
                     /locus_tag="J1138_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBA8452269.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAMTTVIKTMDDIADSTQR
                     IGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGEVRHLASRSANAANDIR
                     KLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQISHSTLEQADGLSSLTRA
                     VDELNLITQKNAELVEESAQVSAMVKHRANRLEDAVTVLH"
     gene            15910..17400
                     /gene="ygjG"
                     /locus_tag="J1138_000424"
     CDS             15910..17400
                     /gene="ygjG"
                     /locus_tag="J1138_000424"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBA8452270.1"
                     /translation="MITTFVFIPIFTIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALAAMRVSVEEA"
     gene            complement(17442..17774)
                     /locus_tag="J1138_000425"
     CDS             complement(17442..17774)
                     /locus_tag="J1138_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBA8452271.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            17993..18976
                     /gene="ebgR"
                     /locus_tag="J1138_000426"
     CDS             17993..18976
                     /gene="ebgR"
                     /locus_tag="J1138_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBA8452272.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            19160..22252
                     /gene="ebgA"
                     /locus_tag="J1138_000427"
     CDS             19160..22252
                     /gene="ebgA"
                     /locus_tag="J1138_000427"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBA8452273.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSAIDHLAIEKLTSARF
                     AFTVEQPQQWSAESPYLYHLVMMLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFVIEQ
                     SDDEVLLVSRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            22249..22698
                     /locus_tag="J1138_000428"
     CDS             22249..22698
                     /locus_tag="J1138_000428"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBA8452274.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            22761..24194
                     /locus_tag="J1138_000429"
     CDS             22761..24194
                     /locus_tag="J1138_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA8452275.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            24328..25398
                     /gene="ygjJ"
                     /locus_tag="J1138_000430"
     CDS             24328..25398
                     /gene="ygjJ"
                     /locus_tag="J1138_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HBA8452276.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAADEARPAEHDDSKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQLFLMQYFYW"
     gene            25415..27766
                     /gene="ygjK"
                     /locus_tag="J1138_000431"
     CDS             25415..27766
                     /gene="ygjK"
                     /locus_tag="J1138_000431"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBA8452277.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSTERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTDNGEMLFTVKKGNKEETQSGLNNYACVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            27992..30010
                     /locus_tag="J1138_000432"
     CDS             27992..30010
                     /locus_tag="J1138_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBA8452278.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDEGLHVVINGEPQILAVDHVVIC
                     AGQEPNRTLAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(30055..30471)
                     /gene="higA"
                     /locus_tag="J1138_000433"
     CDS             complement(30055..30471)
                     /gene="higA"
                     /locus_tag="J1138_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBA8452279.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(30468..30551)
                     /locus_tag="J1138_000434"
                     /pseudo
     CDS             complement(30468..30551)
                     /locus_tag="J1138_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            complement(30742..31878)
                     /gene="rlmG"
                     /locus_tag="J1138_000435"
     CDS             complement(30742..31878)
                     /gene="rlmG"
                     /locus_tag="J1138_000435"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBA8452280.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLCLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            31963..32466
                     /locus_tag="J1138_000436"
     CDS             31963..32466
                     /locus_tag="J1138_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBA8452281.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            32543..33235
                     /locus_tag="J1138_000437"
     CDS             32543..33235
                     /locus_tag="J1138_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBA8452282.1"
                     /translation="MLRVFAHLLRRVCFSRRTLKIGCLLLLIAGATLFIADRVMVNAS
                     QQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAELYRAGKVKWLLVSSDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPTKE"
     gene            33314..34300
                     /locus_tag="J1138_000438"
     CDS             33314..34300
                     /locus_tag="J1138_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA8452283.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPTFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            34583..35548
                     /gene="alx"
                     /locus_tag="J1138_000439"
     CDS             34583..35548
                     /gene="alx"
                     /locus_tag="J1138_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBA8452284.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKLRVG"
     gene            35872..37116
                     /gene="sstT"
                     /locus_tag="J1138_000440"
     CDS             35872..37116
                     /gene="sstT"
                     /locus_tag="J1138_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBA8452285.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILAGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIHFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(37120..37671)
                     /locus_tag="J1138_000441"
     CDS             complement(37120..37671)
                     /locus_tag="J1138_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBA8452286.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(37754..39241)
                     /locus_tag="J1138_000442"
     CDS             complement(37754..39241)
                     /locus_tag="J1138_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBA8452287.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(39256..40668)
                     /gene="uxaC"
                     /locus_tag="J1138_000443"
     CDS             complement(39256..40668)
                     /gene="uxaC"
                     /locus_tag="J1138_000443"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBA8452288.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            41151..42449
                     /locus_tag="J1138_000444"
     CDS             41151..42449
                     /locus_tag="J1138_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452289.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            42579..43355
                     /gene="exuR"
                     /locus_tag="J1138_000445"
     CDS             42579..43355
                     /gene="exuR"
                     /locus_tag="J1138_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBA8452290.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            43700..44362
                     /locus_tag="J1138_000446"
     CDS             43700..44362
                     /locus_tag="J1138_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA8452291.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGILYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            44366..44749
                     /gene="mzrA"
                     /locus_tag="J1138_000447"
     CDS             44366..44749
                     /gene="mzrA"
                     /locus_tag="J1138_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBA8452292.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            44896..45264
                     /locus_tag="J1138_000448"
     CDS             44896..45264
                     /locus_tag="J1138_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBA8452293.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            45302..45607
                     /locus_tag="J1138_000449"
     CDS             45302..45607
                     /locus_tag="J1138_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBA8452294.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRNKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            45610..46014
                     /locus_tag="J1138_000450"
     CDS             45610..46014
                     /locus_tag="J1138_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452295.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLVIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            46004..46303
                     /locus_tag="J1138_000451"
     CDS             46004..46303
                     /locus_tag="J1138_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452296.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            46400..46882
                     /locus_tag="J1138_000452"
     CDS             46400..46882
                     /locus_tag="J1138_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBA8452297.1"
                     /translation="MILSIDSNDANSAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITGYAGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGMNSLMFMKNLTISGGFLLLAITGPGAFSIDRLLNKKW
                     "
     gene            46952..47938
                     /locus_tag="J1138_000453"
     CDS             46952..47938
                     /locus_tag="J1138_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBA8452298.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            48221..48589
                     /locus_tag="J1138_000454"
     CDS             48221..48589
                     /locus_tag="J1138_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA8452299.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(48623..49519)
                     /locus_tag="J1138_000455"
     CDS             complement(48623..49519)
                     /locus_tag="J1138_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8452300.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            49624..50325
                     /locus_tag="J1138_000456"
     CDS             49624..50325
                     /locus_tag="J1138_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBA8452301.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            50348..50512
                     /locus_tag="J1138_000457"
     CDS             50348..50512
                     /locus_tag="J1138_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452302.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(50646..51956)
                     /locus_tag="J1138_000458"
     CDS             complement(50646..51956)
                     /locus_tag="J1138_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBA8452303.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGHYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(51984..53315)
                     /locus_tag="J1138_000459"
     CDS             complement(51984..53315)
                     /locus_tag="J1138_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA8452304.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(53591..54955)
                     /gene="tdcG"
                     /locus_tag="J1138_000460"
     CDS             complement(53591..54955)
                     /gene="tdcG"
                     /locus_tag="J1138_000460"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBA8452305.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(55027..55416)
                     /locus_tag="J1138_000461"
     CDS             complement(55027..55416)
                     /locus_tag="J1138_000461"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBA8452306.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(55430..57724)
                     /gene="tdcE"
                     /locus_tag="J1138_000462"
     CDS             complement(55430..57724)
                     /gene="tdcE"
                     /locus_tag="J1138_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBA8452307.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPIRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(57758..58966)
                     /gene="tdcD"
                     /locus_tag="J1138_000463"
     CDS             complement(57758..58966)
                     /gene="tdcD"
                     /locus_tag="J1138_000463"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBA8452308.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDNVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(58992..60323)
                     /gene="tdcC"
                     /locus_tag="J1138_000464"
     CDS             complement(58992..60323)
                     /gene="tdcC"
                     /locus_tag="J1138_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBA8452309.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(60345..61334)
                     /gene="tdcB"
                     /locus_tag="J1138_000465"
     CDS             complement(60345..61334)
                     /gene="tdcB"
                     /locus_tag="J1138_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBA8452310.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(61433..62371)
                     /gene="tdcA"
                     /locus_tag="J1138_000466"
     CDS             complement(61433..62371)
                     /gene="tdcA"
                     /locus_tag="J1138_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBA8452311.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
     gene            62561..>62724
                     /locus_tag="J1138_000467"
     CDS             62561..>62724
                     /locus_tag="J1138_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312026.1"
                     /note="participates in controlling the expression of
                     several structural genes for the enzyme threonine
                     dehydratase; TdcR activates the tdcABCDEFG operon; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8452312.1"
                     /translation="MTGITIFYGDNIILYVVNTKKGLRLYFKQLPDNYQAKFELNLMS
                     KFSNFIINKP"
BASE COUNT        15282 a        16548 c        15880 g        15014 t
ORIGIN      
        1 gagcgtcgca tcaggcgttg attgccggat gcggcgtgaa cgccttatcc ggcctacgca
       61 atctgataag acgcgtcagc atcatatccg acactgttct tcacatcaaa agaacacaac
      121 gtacaccgca gccgccacaa taaagcgata aatggcgaac gggataaacg aaatgcgctt
      181 aatcaattgc aggaaggttt taatcgctat cagcgccacc acaaaagcgg tgataaaccc
      241 aacggcaaac atcgggatat cgccggtagt caggaagccc cagcttttgt agagatcgag
      301 cgccgttgcg cccatcatca tcggcaccgc cagcaggaac gagaactcgg aagcagcgta
      361 acggctcacc cccatcagca tcccacctga aatggtcgcc ccggaacggg agaaacccgg
      421 ccacagcgcc agacactgga aacagccaat cataaacgcc tgacgatagg tcatatcatc
      481 aagacccggc gcacgcggct ctttcggctt caggcattcg gcggcaatca gcaacagacc
      541 gccaacgacc agtgcataca tgacatttat cgggttaaac aatgatttaa tcgtgtcgtg
      601 gaacaacagc cccagtacca ccgccggaat catccccagc aagatgtgga tcagcgttaa
      661 acgacctttg ctttctcctt cgtgctgcaa cgggcgacca aagtggatgc caatcaggcc
      721 aaacagacgc cgccagaaca tcactactac cgccagaatt gatcctaact ggatcacgac
      781 ttcaaaggtt ttcgccgtgt cgccctcaaa ccccaacaag tgaccgacaa taatcatatg
      841 gcccgtgctg gataccggca gaaattctgt caatccttcg accacaccca atattgccgc
      901 tatcagcagc gagtgcatat cgctcatcaa taaaccccta aattataaaa atgtaacgct
      961 tgtccgaact actgcagtat gaccaggtta taatcgtttg gtttaacagc tgtaaaatta
     1021 attattttct ttcagattat tgccacgctc aatgattacg ccaacattcg ccgcccgcgc
     1081 cactgcgcct ggcttgctga gtttgatacg cacccacggc gagttgaagc gtgctaacag
     1141 cagctccgcc acctcttcag ccacgcgttc caccagcgca aaacgcgctc cctcgacgtg
     1201 gctgaccacc gtttctgcaa tgtcagcgta actgaggcaa tccgccacat catcactttt
     1261 cgccgcttta cggttatccc acgccatttc gatatcgaac actaacttct gttcgatggt
     1321 ctgttcccag tcgtaaacac caatagtggt gattaccgaa agttgctcta taaatacaat
     1381 atccatcacg tcctgcctgc tttttggcta accggatacc acttccggcg aaatgtgcgt
     1441 attatccaca gattcatcgt tgaacacgaa ttttcaaaac ggaacagctt atgagtgcaa
     1501 tcgcgcctgg aatgatcctc atcgcgtacc tctgcggctc catttccagt gccattctgg
     1561 tttgccgctt gtgtgggttg cccgatccgc gaaccagcgg ctccggcaat ccaggcgcaa
     1621 ccaatgtgtt acgtatcggt ggcaagggag cagccgtagc agtactgatt ttcgacgttc
     1681 tgaaaggaat gttgcccgtc tggggcgcgt atgaattagg tgtcagcccc ttctggctag
     1741 gcttaattgc catcgccgcc tgtcttggac acatctggcc cgttttcttc ggttttaaag
     1801 gaggaaaagg cgttgctacc gcttttggtg ccatcgcacc aattggctgg gatctcactg
     1861 gagtaatggc gggaacctgg ttactgaccg tgctattgag tggttactcg tcgctgggag
     1921 cgattgtcag tgcactgatt gctccgtttt atgtctggtg gtttaagcca caattcacct
     1981 tcccggtttc gatgctctct tgcctgatcc tgctgcgtca tcatgacaac atccagcgtc
     2041 tgtggcgtcg tcaggagaca aaaatctgga cgaaattcaa aagaaagcgc gaaaaggatc
     2101 ccgagtgatt tctggtggat ctacatgacc tgatagcctt cgtcgggctt gcccagccgt
     2161 tgctggcacc atgccgccag aaattccaca cagacacgta atttcatgct gcgatagagc
     2221 ggctcccggt aaacagccca gatattggcg ctttgtgcat actccggcaa tactcgcacc
     2281 agtttgccac tctccagaaa cggcaacaca tcccactcgg aacgcagcat aatccctttg
     2341 ccctccagcg cccattgcag cacaatctcg ccgctattgg aggaaagatg tccgcttacc
     2401 ttcaccgatt ttttctcctg accgttcccc aactcccata ttccgtgggt catatcgcgc
     2461 tctttggtca ccaggcagtc atgacgactt aattcctgta aggattgtgg ctgcggatat
     2521 ttttgcagat actcaggtgc tgcgcataat attcttttat tttttgtcaa aagatgcgca
     2581 atataataat caggaattgc gtcattaata cgaatatcca gatcaatatt atcctacact
     2641 aaatcaattt gtcgatcgaa caattcaaaa tgcacctgta actcaggata attgcgcatc
     2701 agttcggtaa tagctggcgc aatatggctg cgcccaaaac caaaactgca accaatgcgg
     2761 atcatcccct ccgggcgcgt tttgatttgc gtgacgtcat cgaccagtcg ctgatattgg
     2821 gtgaggattt ccagcgcatg ttcgtaacaa cgctggccac tttcagtcag cgccacgccg
     2881 cgagcggagc ggttcagcag agttgttgcc agcgtatttt caaggatttg gatccgcttg
     2941 gtgacaaatg caggcgtttg cccaagcgtc gccgctgcag cactaaaact cccgctatgg
     3001 acgatttcca ccagtacctg caagtctttg gctaaaggcc agctatttag cattgttaaa
     3061 tatccgcagt tttgataaac gcagtgtatt tcgcaaaaca ttaccattcc ctgctctggc
     3121 gcaatattcg ttcttatttt cgcgttgatt cacgaactgt gaattacata ttcacggtgg
     3181 caaaaaatat aaaaccacat ttttagtggt agtttgtggc ggtgaatttt tccagacaaa
     3241 tacataaact ggagttgcca tgatgagcga aagtaataag caacaggcag tgaataagtt
     3301 gacggagatt gtcgctaact ttaccgccat gatttctacc cgaatgcctg atgacgtggt
     3361 ggataaacta aaacagctaa aggatgccga aacatcgtcg atgggaaaaa tcatctacca
     3421 cacgatgttc gataacatgc aaaaagcgat cgacctgaat cgtcctgcct gtcaggacac
     3481 cggcgaaatc atgttttttg ttaaggtcgg ttcccgtttc ccactgcttg gcgagctgca
     3541 aagcatactc aaacaagccg tggaagaggc aaccgtcaaa gcgccactgc gtcacaatgc
     3601 ggtagaaatt tttgacgaag taaacaccgg caaaaatacc ggcagtggcg taccgtgggt
     3661 cacctgggat atcgtccccg acggtgacga tgcggaaatc gaagtttaca tggcaggcgg
     3721 cggctgcacg ctacctggcc gatcgaaagt gttaatgccg tcagaaggct acgaaggcgt
     3781 agtgaaattc gtcttcgaaa atatctccac cctcgccgta aacgcctgtc cgccggtact
     3841 tgtgggcgtt ggcatcgcta cctcggtgga aaccgccgca gtgctctcgc gtaaagccat
     3901 tttgcgcccg attggcagcc gccatcccaa tccaaaagcg gcagagctgg agctacgcct
     3961 ggaagaagga ctcaaccgtc tggggattgg tccacaaggg ctgactggca acagttcagt
     4021 gatgggcgtg catatcgaat ctgccgctcg ccatccgtca accatcggcg ttgctgtttc
     4081 taccggctgc tgggcgcatc gtcgcggcac actgcgagtt catgccgatc tcacctttga
     4141 aaatctgtct catacccgga gcgcgttatg aaaaagatcc tgacaacccc gatcaaagct
     4201 gaagatctgc aagatattcg cgtcggcgat gtgatctacc tgaccggtac gctggtgacc
     4261 tgccgtgacg tttgtcaccg ccgattgatc gaactgaaac gtccgatccc ttacgatctc
     4321 aacggcaaag cgattttcca cgctggcccc atcgtgcgca aaaacggcga caaatgggag
     4381 atggtgtccg tcggcccgac aaccagtatg cgtatggaaa gttttgaacg tgaatttatc
     4441 gagcagaccg gcgtgaaact ggtggtcggc aaaggcggaa tggggccgct gaccgaagaa
     4501 ggctgccaga aattcaaggc gctgcatgtg attttcccgg ctggctgtgc ggtactggcg
     4561 gcaactcaag tggaagagat tgaagaggta cactggacag aactcgggat gccagagtcg
     4621 ctgtgggtct gccgggtcaa agagtttggc ccgctgattg tctctataga tacccacggc
     4681 aacaacctga tagccgaaaa caaaaagctg ttcgccgaac gccgcgatcc catcgtagaa
     4741 gagatctgcg agcacgtcca ctacatcaaa taaccctccc ggagaggctc gcccctctcc
     4801 tttttctcag gcataacacg atgaaacctt ccactgaatg gtggcgatac cttgcgccgc
     4861 tggcggtcat cgccattatt gctctacttc cgcttcccgc agggctggag agtcatacct
     4921 ggctctactt tgccgttttt actggcgtga tcgttggact gatcctcgaa cccgtgccgg
     4981 gtgccgtggt ggcgatggtg ggtatatcca tcatcgccat tctttcgccg tggctgctgt
     5041 ttagcccgga gcagctcgcc cagccaggtt ttaagttcac cgccaaatcc ctctcctggg
     5101 cggtttctgg tttttctaat tcagttatct ggcttatttt cgccgccttt atgtttggta
     5161 cgggctatga aaaaaccggg cttggacgtc gcatcgctct gattctggtg aaaaaaatgg
     5221 ggcatcgcac gctgtttctc ggctatgcag tgatgttctc cgagctgata ctcgcgcctg
     5281 taacaccgtc caactcggcg cgtggtgcgg ggattatcta tcccatcatc cgtaacctgc
     5341 caccgctcta tcaatcacaa ccaaacgaca gcagttcgcg cagcattggc tcgtacatca
     5401 tgtggatggg gattgttgcc gattgcgtga ccagcgccat tttcctgacg gcgatggctc
     5461 caaatttgct gttaattgga ctgatgaaaa gcgcatctaa cgccacgctg agctggggcg
     5521 actggttcct cgggatgttg ccgctcagta ttttactggt tctgctggtt ccctggctgg
     5581 cttacgtgct gtacccgccg atactgaagt ctggcgatca ggtgccgcgc tgggcagaga
     5641 cggaactgca ggcaatgggc ccgctctgtt cgcgtgaaaa acggatgctg gggctgatgg
     5701 taggcgcact ggtgctgtgg atttttggcg gtgattatat cgatgccgcg atggtcggtt
     5761 acagcgtggt ggcgctgatg ctgcttctgc gcattatctg ctgggacgac attgtcagta
     5821 ataaagcggc gtggaacgtt ttcttctggc tggcctcgct tatcaccctc gctaccggac
     5881 tcaacaacac cggttttatt agctggtttg gcaaactgtt agcaggcagc ttaagcggtt
     5941 attcgccaac gatagtgatg gtggcgttga ttgtggtctt ttatctgctg cgctactttt
     6001 ttgccagcgc cacagcgtat acctccgctc tcgcaccgat gatgattgcc gccgcgctgg
     6061 cgatgccgga aatcccgctg ccggtgttct gcctgatggt tggtgcggca attggtctgg
     6121 ggagcattct tacaccatac gccaccggac ccagtccgat ttactacggc agtggttatc
     6181 tgccaacggc ggattactgg cgactggggg cgatttttgg gctgatattc ctcgtattgc
     6241 tggtgattac cggcttactg tggatgcccg tggtgttgct ttaagtgagg acggtgccgg
     6301 aaattgctct ccggcaccca atagttttac gcagccggta actccgccag cggccagcgc
     6361 ggacgcacac tcacgccgag atccgccgtc gcgcctgctt taaaccgcac cattccggca
     6421 taggcgatca tcgcaccgtt atcagtgcaa aattccggac gcgcgtagaa cacttcgcca
     6481 cgacgctttt tcatcatttc agccagcttc gcccgcagtg tgcggttagc actcacgccg
     6541 cctgccatga ccagtcgctt aaagcccgtc tgatccagtg ctcgcttaca cttaatcatc
     6601 aacgtatcga ccactgcatc ttcaaaggcg cgggcgatat cggcacgcgt ctgatcgtca
     6661 gtgccgttgt cacgtatggt atttgccgcg aaggttttca gaccagaaaa gctgaaatcc
     6721 agccccggac ggtcggtcat cgggcgcggg aagacaaagc gcccggcagt accctgagcc
     6781 gccattttcg acagtaacgg accgccagga taatccagcc ccagcagctt cgcggtttta
     6841 tcaaacgctt ccccggcggc atcatcgata gactctccga gcagctcgta ctgaccaatg
     6901 ccagtcacgc tgattaactg cgtatgaccg ccggaaacca gcagcgcgac aaacggaaat
     6961 tccggtgggt tatcttccag catcggcgct aacagatgcc cttccatatg gtgtaccggg
     7021 atcgccggaa cgccccaggc aaacgccaga gaacgcccca cggtcgcgcc aaccagtagc
     7081 gcgccgacta atccagggcc tgcggtatag gccacagcat caatgtcttt tgccgttaaa
     7141 ccagactcct ttagcgccgc ctggatcaac ggtacggttt tacgcacatg atcgcgggag
     7201 gccagctcag gcacgacgcc gccgtagtca gcgtgcaatt tcacctgact atacaattgg
     7261 ttggctaaca aacctttttc atcgtcgtaa atggcgatgc cggtttcatc gcaggaagtt
     7321 tcaataccca gtacacgcat gacttgtttt acctcgcttt attaccgcgc agtgtaggac
     7381 caatgcgggt tgatgtaaaa ctttgttcgc ccctggagaa agcctcgtgt atactcctca
     7441 cccttataaa agtccctttc aaaaaaggcc gcggtgcttt acaaagcagc agcaattgca
     7501 gtaaaattcc gcaccatttt gaaataagct ggcgttgatg ccagcggcaa accgaattaa
     7561 tcaaaggtga gaggcacatg ccggtaatta aagtacgtga aaacgagccg ttcgacgtag
     7621 ctctgcgtcg cttcaagcgt tcctgcgaaa aagcaggtgt tctggcggaa gttcgtcgtc
     7681 gtgagttcta tgaaaaaccg actaccgaac gtaagcgcgc taaagcttct gcagtgaaac
     7741 gtcacgcgaa gaaactggct cgcgaaaacg cacgccgcac tcgtctgtac taattccccg
     7801 agagcgttgc tctccgatca gaccgagttg tagttgtaag gccgtgcttc cgaaaggaat
     7861 gcgcggctta ttttcgttta tgaattgcta aaaatcgggg cctatggctg gacgaatccc
     7921 acgcgtattc attaatgatc tgctggcacg cactgacatc gtcgatctga tcgatgcccg
     7981 tgtgaagctg aaaaagcagg gcaagaattt ccacgcgtgt tgtccattcc acaacgagaa
     8041 aaccccgtca ttcaccgtta acggtgagaa acagttttac cactgctttg gatgtggcgc
     8101 gcatggcaac gcgatcgact tcctgatgaa ctacgacaag ctcgagttcg tcgaaacggt
     8161 cgaagagctg gcagcaatgc acaatcttga agtgccattt gaagcaggca gcggccccag
     8221 ccagatcgaa cgccatcagc ggcaaacgct ttatcagttg atggacggtc tgaatacgtt
     8281 ttaccaacaa tctttacaac aacctgttgc cacgtctgcg cgccagtatc tggaaaaacg
     8341 cggattaagc cacgaggtta tcgcccgctt tgcgattggt tttgcgcccc ccggctggga
     8401 caacgtcctg aagcggtttg gcggcaatcc agaaaatcgc cagtcattga ttgatgcggg
     8461 catgttggtc actaacgatc aggggcgcag ttacgatcgt ttccgcgagc gggtgatgtt
     8521 ccccattcgc gataaacgcg gtcgggtgat tggttttggc gggcgcgtgc tgggcaacga
     8581 tactcccaaa tacctgaact cgccggaaac ggacattttc cataaaggcc gccagcttta
     8641 cggtctttat gaagcgcagc aggataacgc tgaacccaat cgtctgcttg tggtcgaagg
     8701 ctatatggac gtggtggcgc tggcgcaata cggcattaat tacgccgttg cgtcgttagg
     8761 tacgtcaacc accgccgatc acatacaact gttgttccgc gcgaccaaca atgtcatttg
     8821 ctgttatgac ggcgaccgtg caggccgcga tgccgcctgg cgagcgctgg aaacggcgct
     8881 gccttacatg acagacggtc gtcagctacg ctttatgttt ttgcctgatg gcgaagatcc
     8941 tgacacgctg gtacgaaaag aaggtaaaga agcgtttgaa gcgcggatgg agcgggcgat
     9001 gccactctcc gcatttctgt ttaacagtct gatgccgcaa gttgatctga gtacccctga
     9061 cgggcgcgca cgtttaagta cgctggcact gccactgata tcgcaagtgc cgggcgaaac
     9121 gctgcgaata tatctacgtc aggaattagg caacaaatta ggcatacttg atgacagcca
     9181 gcttgaacga ttaatgccaa aagcggcaga gagcggcgtt tctcgccctg ttccgcagct
     9241 aaaacgcacg accatgcgta tacttatagg gttgctggtg caaaatccag aattagcgac
     9301 gttggtcccg ccgcttgaga atctggatga aaataagctc cctggacttg gcttattcag
     9361 agaactagtc aacacttgtc tctcccagcc aggtctgacc accgggcaac ttttagagca
     9421 ctatcgtggt acaaataatg ctgccaccct tgaaaaactg tcgatgtggg acgatatagc
     9481 agataagaat attgctgagc aaaccttcac cgactcactc aaccatatgt ttgattcgct
     9541 gcttgaactg cgccaggaag agttaatcgc tcgtgagcgc acgcatggtt taagcaacga
     9601 agaacgcctg gagctctgga cattaaacca ggagctggcg aaaaagtgat ttaacggctt
     9661 aagtgccgaa gagcgatcgg gaagcccccg acagccgcac tgagaggcag cggcaaatat
     9721 ataagtacgc cctcgtaatt atcgttggcg gtaaacaacc gttggatttc agcgttaacg
     9781 gctgaaggac atcggatcaa tcgcccaaca ccaacctcat gaaataagtg tggataccgt
     9841 cttatggagc aaaacccgca gtcacagctg aaacttcttg tcacccgtgg taaggagcaa
     9901 ggctatctga cctatgccga ggtcaatgac catctgccgg aagatatcgt cgattcagat
     9961 cagatcgaag acatcatcca aatgatcaac gacatgggca ttcaggtgat ggaagaagca
    10021 ccggatgccg atgatctgat gctggctgaa aacaccgcgg acgaagatgc tgccgaagcc
    10081 gccgcgcagg tgctttccag cgtggaatct gaaatcgggc gcacgactga cccggtacgc
    10141 atgtacatgc gtgaaatggg caccgttgaa ctgttgaccc gcgaaggcga aattgacatc
    10201 gctaagcgta ttgaagacgg gatcaaccag gttcaatgct ccgttgctga atatccggaa
    10261 gcgatcacct atctgctgga acagtacgat cgtgttgaag cagaagaagc gcgtctgtcc
    10321 gatctgatca ccggctttgt tgacccgaac gcagaagaag atttggcacc taccgccact
    10381 cacgtcggtt ctgagctttc ccaggaagat ctggacgatg acgaagatga agacgaagaa
    10441 gatggtgacg acgataccgc cgatgatgac aacagcatcg acccggaact ggctcgcgaa
    10501 aaatttgcgg aactgcgcgc tcagtacatt gtaacgcgtg acaccatcaa agcaaaaggc
    10561 cgcagtcacg ctgccgctca ggaagagatc ctgaaactgt ctgaagtatt caaacagttc
    10621 cgcctggtgc cgaagcagtt tgactacctg gtcaacagca tgcgcgtcat gatggaccgc
    10681 gttcgtacgc aagaacgtct gatcatgaag ctctgcgttg agcagtgcaa aatgccgaag
    10741 aaaaacttca ttaccctgtt taccggcaac gaaaccagcg atacctggtt caacgcggca
    10801 attgcgatga acaagccgtg gtcggaaaaa ctgcacgatg tctctgaaga agtgcatcgc
    10861 gccctgcaga aactgcagca gattgaagaa gaaaccggcc tgaccatcga gcaggttaaa
    10921 gatatcaacc gtcgtatgtc catcggtgaa gcgaaagccc gccgtgcgaa gaaagagatg
    10981 gttgaagcga acttacgtct ggttatttct atcgccaaga aatacaccaa ccgtggcttg
    11041 cagttccttg acctgattca ggaaggtaac atcggcctga tgaaagcggt tgataaattc
    11101 gaataccgcc gtggttacaa gttctccacc tacgctacct ggtggatccg tcaggcgatc
    11161 acccgctcta tcgcggatca ggcgcgcacc atccgtattc cggtgcatat gattgagact
    11221 atcaacaagc tcaaccgtat ttctcgccag atgctgcaag agatgggccg tgaaccaacg
    11281 ccggaagaac tggctgaacg tatgctgatg ccggaagaca agatccgtaa agtgctgaag
    11341 atcgccaaag agccaatctc catggaaacg ccgatcggtg atgatgaaga ttcgcatctg
    11401 ggggatttca tcgaggatac caccctcgag ctgccgctgg attctgcgac caccgaaagc
    11461 ctgcgtgcgg caacgcacga cgtgctggct ggcctgaccg cgcgtgaagc gaaagttctg
    11521 cgtatgcgtt tcggtatcga tatgaacacc gaccacacgc tggaagaagt gggtaaacag
    11581 ttcgacgtta cccgcgaacg tatccgtcag atcgaagcga aggcgctgcg caaactgcgt
    11641 cacccgagcc gttctgaagt gctgcgtagc ttcctggacg attaatcggt aggccggatc
    11701 aggtgttacg ccgcacccgg cactagtccc tccgcacaaa cgccaccttt tcggtggcgt
    11761 ttttatcgcc cacgcaccac cagcgcctgg tccagctcgc gatacgcttc aaccagtttc
    11821 tccagtgaaa cgcgacttaa accgctggga tttggcagca cccaaatctg cgtcgaacca
    11881 atggtgagag tttgtttccc ccactgtgca ccgcgctggc tgaatccctg ttcatatgct
    11941 tgtttgccca gaatcgccaa cgcctgcggc tgataatctt ctattttttc aattagctta
    12001 cgcccgcctg cgtgcagctc ctgctttgac acttcattgg cttgcaccgt tggacggtct
    12061 accagtttgg tgacgccaca acgataatcc agcagatgct gtgcctcctg cggcttcaac
    12121 tgacggtcgg taaacccggc ctgatatatc accttccaga agcggtttgc cggatgagca
    12181 aagggaaaac ccgttccggc ggatgaaaga ccagggttga taccgcaaaa cacgacccgt
    12241 aaccctggag ccaaaatatc ctcaaccata tttactcctt tctaacatca ccaggaaaag
    12301 tataacggat tgaaaataca ttgtttataa aaacagcagg cgcgcggtaa tggctggatt
    12361 gcgacacgga gttactttat aatccgctac catggcccct tagctcagtg gttagagcag
    12421 gcgactcata atcgcttggt cgctggttca agtccagcag gggccaccag acatagcaaa
    12481 ggctgacgag aaatcgtcag cctttttctt tttatatatc agttactttg cgtgccagta
    12541 agccgctgca cgtacccgct gtgggtcata ctgttccgct tcaaagcggc ggcttaaatt
    12601 cttaacgact ttgccttcgc cggttatcca gatgaagtaa tcatcggcag ggatttgcat
    12661 ctgcgccaga cgcgcatcta ccgcctgctc atcatgtgcc agccattcga tattaaaacc
    12721 atcaaggtgc gcgagataat cctgacaggc gttatcccgc acgctaacca gcgcactaac
    12781 ttgcggttta acggcaagtt tgctcaacgt ttccaggcgg tggcgcagtg cgggcattcc
    12841 ggattcatcg cagacataca gctgatacgc gtaatcctct ggcaccacca gcgaaccgcg
    12901 cggacctgcc accgtaagtt tatcgcccgg tttcgcctgc atcgcccagc cgctggcgac
    12961 cccaccgtcg tgaataaaga aatcgatcgc cagttcatgg cgtagttcgt catacagcgg
    13021 cgtatagtca cgcgacggtg ggcgtggtcc ttccggccag acgatgccct cttccgttac
    13081 cgttggcggc acaaagtgag tgcctggttg aggaaagaag agcttgctgt gatcgtcaaa
    13141 gccgcgcgat gtaaaaccgt ccagcgcctc gccgccaagg acaatgcgct gaaaaccggc
    13201 gctgatacgc tcaacgcgta gcacagtcag ttcacggaag cgcagatcat tgcgaacgcg
    13261 ctgcgggtag cggggggagt tattcattgt tatcgccttc atgatggtaa tcagatatat
    13321 ctaaataaat tccacaaatg ataatgattg ttaatcatga taaatgcaag tgatttgtag
    13381 cactgatata tctgtagtct gataagacgc gcaacctctt atcaagcatc attgctcaat
    13441 gccggatgcg gcgtgaacgc cttatccggc ctacatgttg agtaaacctg cgtttaaaaa
    13501 tcatttttgt acttgcagta atgcttatat cagatataaa ttagatatat ctaattaagc
    13561 aaaaggaggc tgatatgagc catcatcacg aagggtgttg taaacatgaa ggccagccac
    13621 gccatgaggg ctgctgcaaa ggtgagaagt cagaacacga gcactgcgga cacggtcacc
    13681 agcatgaaca cggtcaatgc tgcggtggtc gccacggtcg cggcggcggt cgtcgacaac
    13741 gtttctttgg tcacggtgaa ttgcgtctgg tgattctgga tattctctcg cgcgatgaca
    13801 gccacggtta cgagttaatt aaagcgattg aaaatctgac ccaggggaat tacaccccaa
    13861 gcccgggcgt catctaccca acgctggatt ttctgcagga gcagtcgctg attaccattc
    13921 gcgaagagga aggtggtaag aagcagattg cgctgaccga acaaggcgcg cagtggctgg
    13981 aagaaaaccg cgaacaggta gagatgattg aagaacgcat caaagcgcgt tgcgttggcg
    14041 ctgcgctgcg ccagaacccg caaatgaagc gggcgctgga taattttaaa gcggtgctgg
    14101 atttacgcgt caaccagagc gatatcactg atgcacaaat aaaaaagatc attgcggtga
    14161 tcgaccgcgc cgcttttgat attacccaac tggattaatc accgattgcc ggatgcagcg
    14221 taccctacac accccgtagg cctgataaga tgcgccagca tcgcatcagg cattgtgctc
    14281 caaccgccgg atccggcata ccgattaatg cagcaccgtc accgcgtctt ccagtcggtt
    14341 ggcgcggtgt ttcaccatcg ccgacacctg cgcactctct tccactaact cggcgttttt
    14401 ctgggtgatc aggttaagct catccactgc acgggtcagg ctggaaagcc catcggcctg
    14461 ttccagcgtt gaatggctaa tctgagctat caactgggtg acgtttttta cctgcgccac
    14521 aatatcttcc atcgtccgtc cggcggcgtg tacctgctgc gaaccagatt gcaccttatc
    14581 agcactggca tcaatcagct tgcgaatatc gttggcagca ttggcgctgc ggctggctaa
    14641 atgacgcact tcccccgcca ccactgcaaa acctttgccc tgttcgccgg cacgcgccgc
    14701 ttccaccgcc gcattcaggg ccagaatatt ggtctgaaac gcaatatcgt taatcagcga
    14761 agtaatggtg ccaatgcgct gggtactgtc ggcaatatcg tccattgtct tgatcaccgt
    14821 agtcatcgcc tccccgccct gcactgccgc attactggcg gtgatagaaa gtttatcggc
    14881 agccgacgcc gtggcagagt tctgtttcac cgacgccgcc atttggttca tggtcgccac
    14941 cttcagtgcc tgacgggcga cattttctat cgggcggaca atctgccatt caaaacaggc
    15001 gcttgccaac aataccacta acgcacagag gatatacgtc accaccgggg cagcaacaaa
    15061 ccaaagcatg accgccagca agataaacat cagggtcatc acgccacgcg cccgccagcg
    15121 aagcggtaat gaaggcagtt tacccagcca gcctttacgc accaccaggc ctttatgaat
    15181 acgcttactg gtacgtccgg cgttcaacgc tttgtacagc ggctccaccg ccgcgatctc
    15241 ttcatccgtc gcccgggtac gaatcgacat atagccactg atttttccct cgcgcaccat
    15301 cggtaccgca ttggcccgca cccaataatg gtcgccattt ttgcggcgat ttttcacgat
    15361 gccgctccag ggctcccctt ttttcagggt gaaccacata tccgcaaacg ccgcttttgg
    15421 catatccggg tgacgcacca tgttgtgcgg ctgcccctgt aactcttgca aggtatagcc
    15481 gctcacctgc acaaaagtgt cattagcatg agtgatatag ctttgcagat cggtagtgga
    15541 catcagagtg gtatcgtccg ccagcggggt attttgctgg gtgacatacg gatgagaaga
    15601 catgttcgcg tcctgtgcag gttatatggt tgttaacttc ttgtcagagt ttatgtcggc
    15661 cccgctgcag ttatctttaa ctgattaatt tgatttagat cgcaatttgc gattaaagca
    15721 taaatctaat cccttgattt aaaatacttt cactctgtta ctatacgaaa acgttaatta
    15781 tcttgcccaa aaatcaggca attattgccc tgaaagcgtg catttgcgca gcaatcatca
    15841 aatccatacc cgacaaaaac cgtgcaaaat aacagcaaat gttagcaaat agcattaaat
    15901 attgcgcaaa tgataaccac atttgtgttt atcccgattt tcacgatcgc agccggagtg
    15961 gcgcaatccc tgcaatactt aaatcggtat catgtgatac gcgagcctcc ggagcatatt
    16021 ttgaacaggt taccttcgag cgcatcggct ttagcgtgca gcgcccacgc cctgaatctc
    16081 attgaaaagc gaacgttgga tcatgaggag atgaaagcac ttaaccggga ggtgattgaa
    16141 tacttcaaag agcatgtcaa tccggggttt ttagagtatc gcaaatctgt taccgctggc
    16201 ggggattacg gagccgtaga gtggcaagcg gggggtttaa atacgcttgt cgacacccag
    16261 ggacaggagt ttatcgactg cctgggaggt tttggaattt tcaacgtggg gcaccgtaat
    16321 ccagttgtgg tttccgccgt acagaatcaa cttgctaaac aaccgcttca cagccaggag
    16381 ttgctcgatc cgttacgggc gatgctggcg aaaacccttg cggcgctaac gcccggtaaa
    16441 ctgaaataca gcttcttctg taatagcggc accgaatccg ttgaagcggc gctgaagctg
    16501 gcgaaggctt atcagtcacc gcgcggcaag tttactttta ttgccaccag cggcgcgttc
    16561 cacggtaaat cacttggcgc gctgtcggcc acggcgaaat cgaccttccg caaacctttt
    16621 atgccactgc tgccagggtt tcgtcatgta ccgtttggca atatcgaagc catgcgcacg
    16681 gctcttaacg agtgcaaaaa aaccggtgat gatgttgctg cggtgatcct cgaaccgatt
    16741 cagggcgaag gtggcgtcat tctgccgccg ccgggatatc tcaccgccgt gcgtaagctg
    16801 tgcgatgagt tcggcgcact gatgatcctc gatgaagtgc aaaccggtat ggggcgcacg
    16861 ggcaagatgt tcgcctgcga gcatgagaac gtacagccgg acatcctgtg tctggcaaaa
    16921 gccctcggcg gcggcgtgat gccgattggc gcaaccatcg ccactgaaga ggtgttttcc
    16981 gttctgttcg acaacccgtt cctgcatacc accacctttg gcggcaaccc gctggcctgt
    17041 gcggcggcgc tggcgaccat caatgtgttg ctggagcaga acttaccggc tcaggctgaa
    17101 caaaaaggcg atatgttgct ggacggtttt cgtcaactgg cgcgggaata tcccgatctg
    17161 gtacaggaag cgcgtggtaa agggatgttg atggcgattg agtttgttga taacgaaatc
    17221 ggctataact ttgccagcga gatgttccgc cagcgcgtcc tggtggccgg aacactcaac
    17281 aacgctaaaa cgatccgcat tgaaccgcca ctgacactga ccattgaaca gtgtgaacag
    17341 gtgatcaaag cggcgcgtaa ggcgctggcg gccatgcgag tcagtgtcga agaagcgtag
    17401 tatcaaatcg gatggcgata cgacgtcgcc atccgatctt tttatacgat acgtacgccc
    17461 gcaggcatca tccgctccgg cgttaacagc acgctttcac tgccgtcatc ggtttccgca
    17521 cacaacagca tgcattccga cgtttcaccg cgcattttcg ctttttgcaa attgcataag
    17581 accaccactg tttttcccat cagctcttct tcgctgtagt acggcaccag gctggtcacg
    17641 gtttgcagcg ttttttcccc cacatcaacc tgtacgatgt acagcttgtc ggcgttttca
    17701 tggcgtttca cttccacaat ctttccgacg cgcatttcca gacgtgcaaa atcagcgtaa
    17761 gccacggttt ccattgtttc ctcccttcga gtaaaatttt actaaactat agcaaaagtt
    17821 tttctcaatc ctgtaggcta aaaatggaga atgcaggcgt gatcacattc gcaagccgct
    17881 gtgttaccgt tacagcgtca aagaaacgcg ctttatttac tgaaaacagg tgacccgata
    17941 agcacttcct ctacaatggg ggcgcacatc agggaaagta aaaaaggtaa acatggcaac
    18001 actaaaagac atcgcaatcg aagctggcgt atccctggcg acagtatcca gggtcttgaa
    18061 tgacgatccg acattgaatg tgaaagaaga gacgaaacat cgcattctcg agatcgccga
    18121 aaagctggag tacaagacca gtagcgcccg taaactccag acaggcgcag tcaaccaaca
    18181 ccatattctg gctatctaca gctaccaaca ggagctggag atcaacgatc cttactatct
    18241 ggcgatccgc cacggcattg aaactcagtg cgaaaagctg gcgatcgaac tgaccaactg
    18301 ttatgaacac aacggcttac cagacattaa aaacgtcacc ggtattttaa ttgtcggcaa
    18361 acccacgtcc gccctgcgcg ccgccgcctg tgcgttgacc gacaatatct gttttatcga
    18421 ctttcacgaa cccggcagcg gttacgatgc ggtggatatc gatctggcac gtatcagtaa
    18481 agaaatcatc gatttctata tcaatcaggg cgttaatcgt attggtttta ttggcggtga
    18541 agacttgcct ggcaaggcgg atattcgtga ggtcgccttt gcggaatatg gtcgtctgaa
    18601 acacgtggta cgcgaagagg atatctggcg cggcggtttt tccagttcgt cgggctatga
    18661 actggcaaaa cagatgctgg cgcgggaaga ctatccgaag gcactgtttg ttgcttccga
    18721 ttccattgct atcggcgtac tgcgggcaat tcatgaacgt ggcctgaaca tcccacagga
    18781 tatttcgctt atcagcgtta acgatatccc caccgcgcga tttacctttc cgccgctctc
    18841 caccgtgcgc atccattccg agatgatggg aagtcagggc gttaacctgg tgtatgaaaa
    18901 agcccgcgat ggtcgcgcgc tgccgctgtt ggtcttcgtt cccagcaaat taaaactgcg
    18961 cggcacgacc cgttaaatcc ccttgcacgc tgtccggcaa tcgtttttgc cggacagtgc
    19021 tgccgtttat tttcgtgatc cagttaaagt aaatgcattt atccgctact ttttagtaaa
    19081 atttttacta aactccccag caattacaca aactaccatc accatgaatg gttccgattt
    19141 ctctctaccg ggaggcccta tgaatcgctg ggaaaacatt cagctcaccc acgaaaaccg
    19201 acttgcgccg cgtgcgtact ttttttcata tgattctgtt gcgcaggctc gtacttttgc
    19261 ccgcgaaacc agcagcctgt ttctgtcctt aagcggtcag tggaatttcc acttttttga
    19321 ccatccgctg caagtgccag aagccttcac ctctgagtta atggctgact gggggcatat
    19381 taccgtcccc gccatgtggc aaatggaagg tcacggcaaa ctgcaatata ccgacgaagg
    19441 ttttccgttc cccatcgatg tgccgtttgt ccccagcgat aacccaaccg gtgcctatca
    19501 acgtattttc accctcagcg acggctggca gggtaaacag acgctgatta aatttgacgg
    19561 cgtcgaaacc tattttgaag tctacgttaa cggtcagtat gtggggttca gcaagggcag
    19621 tcgcctgacc gcagagtttg acatcagcgc gatggttaaa accggcgaca acctgttgtg
    19681 tgtgcgcgtg atgcaatggg cggactctac ctacgtggaa gaccaggata tgtggtggtc
    19741 ggcggggatt ttccgcgatg tttatctgat cggaaaacaa ctcacgcata ttaacgattt
    19801 caccgttcgt accgactttg acgaagccta ttgcgatgcc acactttcct gcgaagtggt
    19861 gctggaaaat ctcgccgcct cccctgtcgt cacgacgctg gaatataccc tgtttgatgg
    19921 cgaacacgtg gtgcacagca gcgccattga tcatctggca attgaaaaac tgaccagcgc
    19981 ccgctttgct tttactgtcg aacagccgca gcaatggtca gcagaatccc cttatcttta
    20041 ccatctggtc atgatgctga aagacgccga cggcaacgtt ctggaagtgg tgccacaacg
    20101 cgttggcttc cgtgatatca aagtgcgcga cggtctgttc tggatcaata accgttatgt
    20161 gatgctgcac ggcgtcaacc gccacgacaa cgatcatcgc aaaggccgcg ccgttgggat
    20221 ggatcgcgtc gagaaagatc tccagttgat gaagcagcac aacatcaact ccgtgcgtac
    20281 cgctcactac ccgaacgatc cacgttttta cgaactgtgt gatatctatg gcctgtttgt
    20341 gatggcggaa accgacgtcg aatcgcacgg ctttgctaac gtcggcgata tcagccgcat
    20401 taccgacgat ccgcagtggg aaaaagttta cgtcgagcgc attgttcgcc atattcacgc
    20461 gcagaaaaac catccgtcga tcatcatctg gtcgctgggc aatgaatccg gctatggctg
    20521 taacatccgc gcgatgtacc atgcggcgaa ggcgctggat gacacgcgac tggtgcatta
    20581 cgaagaagat cgcgatgctg aagtggtcga tattatttcc accatgtaca cccgcgtgcc
    20641 tctgatgaat gagtttggtg aatacccgca tccgaagccg cgcatcatct gtgaatatgc
    20701 tcatgcgatg gggaacggac cgggcgggct gacggagtac cagaacgtct tctataagca
    20761 cgattgcatt cagggacatt atgtctggga atggtgcgac cacgggatcc aggcgcagga
    20821 tgacaacggc aacgtctggt acaaattcgg cggcgactac ggcgactatc ccaacaacta
    20881 taacttctgt cttgatggtt tgatctattc cgatcagacg ccgggaccag gcctgaaaga
    20941 gtacaaacag gttattgcgc cggtaaaaat ccacgcgctg gatctgactc gcggcgagtt
    21001 gaaagtcgaa aataaactat ggtttaccac gcttgatgac tacaccctgc acgcagaggt
    21061 gcgcgccgaa ggtgaaacgc tcgcgacgca acagattaaa ctgcgcgacg ttgcgccgaa
    21121 cagcgaagcc cccttacaga tcacgctgcc gcagctggac gcccgcgaag cgttcctcaa
    21181 cattacggtg accaaagatt cccgcacccg ctacagcgaa gccgggcatt ctatcgccac
    21241 ttatcagttc ccgctgaagg aaaacaccgc gcagcctgta cctttcgcac cgaataacgc
    21301 ccgtccgctg acgctggaag acgatcgttt gagctgtact gttcgcggct acaactttgc
    21361 gatcaccttc tcaaaaacga gtggtaaacc gacatcctgg caagtgaatg gcgagtcgct
    21421 gctgacccgc gagccaaaga tcaacttctt caagccgatg atcgacaacc ataagcagga
    21481 gtacgaaggg ctgtggcagc caaatcattt gcagatcatg caggagcatc tgcgcgattt
    21541 tgtcattgag caaagtgatg atgaagtgtt gctagtcagc cgcacggtta ttgccccacc
    21601 ggtgtttgac ttcgggatgc gctgcaccta catctggcgc atcgctgccg atggccaggt
    21661 taatgtggcg ctttccggcg aacgttatgg cgactatccg cacatcattc cgtgcatcgg
    21721 tttcaccatg ggaattaacg gcgaatacga ccaggtggcg tattacggtc gtggaccggg
    21781 cgaaaactac gccgacagcc agcaggctaa catcatcgat atctggcgca gcaccgtcga
    21841 tgccatgttc gagaactatc ccttcccgca gaacaacggt aaccgtcagc atgtccgctg
    21901 gacggcactg actaaccgcc acggcaacgg tctgctggtg gttccgcagc gaccaattaa
    21961 cttcagcgcc tggcactata cccaggaaaa catccacgct gcacagcact gtaacgagct
    22021 gcagcgcagt gatgacatca ccctgaatct cgaccaccag ctgcttggcc tcggctccaa
    22081 ctcctggggc agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact tcagctacgg
    22141 ctttacgttg ctgccggttt ctggcggaga agctaccgcg caaagcctgg cgtcgtatga
    22201 gttcggcgca gggttctttt ccacgaattt gcacagcgag aataagcaat gaggatcatc
    22261 gataacttag aacagttccg ccagatttac gcctctggca agaagtggca acgctgcgtt
    22321 gaagcgattg aaaatatcga caacattcag cctggcgtcg cccactccat cggtgactca
    22381 ttgacttacc gcgtggagac ggactccgcg accgatgcgc tatttaccgg gcatcgacgc
    22441 tattttgaag tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc gccgaaagag
    22501 acattacagg tagtggaata ttatcgtgat gaaactgacc gtgaatattt aaaaggctgc
    22561 ggagaaaccg tagaggtcca cgaagggcaa atcgttattt gcgatatcca tgaagcgtat
    22621 cggtttatct gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat cgaagatggt
    22681 tatttccata acaaataaca actacggcgg caaaaggagt ttgccgccac cgctacccta
    22741 ctcattttcg gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc aaattcggct
    22801 tgctctcgct gacttttgcc gccgtttaca gctttaacaa cgttatcaat aataatattg
    22861 agcttggact ggcctcggca ccgatgtttt tcctcgcgac gattttttat tttattccct
    22921 tctgtctgat catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc ggtgtctacg
    22981 cgtgggtaaa aagttcgctg ggcggacgtt gggcatttat tactgcctat acctactggt
    23041 tcgtaaacct gttctttttc acctcgctgt taccgcgcgt tattgcttat gcttcgtatg
    23101 ccttcctcgg ctacgaatat attatgacgc cggttgccac caccattatc agtatggtgc
    23161 tgttcgcctt ctccacctgg gtttctacca atggggcgaa aatgctgggg ccaattacct
    23221 ccgtcacttc aacgctgatg ctgctgttaa cgctctccta cattttactg gcaggtacgg
    23281 cactggttgg cggcgtacag cctgccgatc cgatcaccgt tgacgcgatg atcccgaact
    23341 tcaactgggc gttcctcggc gtgaccacct ggatctttat ggccgcaggt ggcgcggagt
    23401 ccgtcgcggt gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt aaagtgatca
    23461 tcctcgccgg gatttttatc ggcgtactgt attccgtctc ctcggtgctg attaacgtct
    23521 tcgtcagcag caaagagttg aaatttactg gcggatcggt gcaggtattc cacggcatgg
    23581 cggcgtattt tggtctaccg gaagcgttga tgaatcgctt tgtcggtctg gtgtccttta
    23641 ccgcgatgtt cggttccctg ctgatgtgga cggcaacgcc ggtgaaaatt ttcttctccg
    23701 aaatcccgga aggcatcttt ggtaagaaaa ccgtcgaact taacgaaaac ggcgttccgg
    23761 cgcgcgcagc gtggatccag ttcctgatcg tcatcccgct gatgattatc ccgatgctcg
    23821 gttccaatac cgtgcaggat ctgatgaata ccattattaa tatgaccgcc gcagcgtcca
    23881 tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa ttagatcacc
    23941 tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt tcaatgctga
    24001 ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg aatattctga
    24061 ccatcatttt ttataacgtc ggcggtattg ttatcttcct cggctttgcg tggtggaaat
    24121 acagtaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg acgccagcca
    24181 gcaatgttga ttaagcagac taataaaaaa tgagcaggca ttttccctct tcctggatga
    24241 ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa aaggctgtac
    24301 tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg cagagcatta
    24361 cttgctctgc cgttttgcta cgccttttct gcggccgctg atgaagcacg tccggcagaa
    24421 catgacgact caaaaacccc cgcaatgacc tcgacatctt ctccttcatt tcgtttttac
    24481 ggcgaattag gggttggtgg gtatatggat ttagagggtg agaataaaca taaatacagc
    24541 gacggcacct atattgaagg tggcctggag atgaagtacg gctcctggtt cggcctgatt
    24601 tacggcgaag gctggaccgt gcaggccgac cacgacggta atgcctgggt gcctgaccat
    24661 agctggggtg gtttcgaagg tgggattaac cgtttctatg gcggttatcg taccaatgat
    24721 ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc tggatgacct gcaatggtgg
    24781 ggcgatttca cgcccgatct gggctacgtc attcccaata cccgcgacat tatgactgcg
    24841 ctgaaggtac agaacttaag cggcaacttt cgttatagcg ttaccgccac acctgccgga
    24901 catcatgacg aaagcaaagc ctggctacat tttggcaagt acgatcgcta cgacgacaaa
    24961 tacacctatc cggcaatgat gaacggttac atccagtatg accttgccga aggcatcacc
    25021 tggatgaacg gtctggaaat caccgacggc acaggacaac tctatctcac gggcctgctt
    25081 acccctaact ttgccgctcg cgcctggcac catacaggac gcgccgacgg gctggacgta
    25141 ccgggaagtg aaagtgggat gatggtgagc gccatgtatg aagcgttaaa gggcgtttat
    25201 ctctccaccg cttacaccta cgccaaacat cgccctgacc acgctgacga tgaaaccacc
    25261 tctttcatgc agtttggtat ctggtacgaa tacggcggcg gacgtttcgc aacggctttt
    25321 gatagccgct tctacatgaa aaatgcctct aacgatccca gcgaccaact cttcctgatg
    25381 caatatttct actggtaata aggactgtaa tttcatgaaa atcaaaacta ttttaacgcc
    25441 agtaacctgc gctctgctga taagtttttc cgcccatgcc gctaacgccg acaattataa
    25501 aaacgtgatt aaccgtactg gcgcgccgca gtacatgaag gattacgatt acgacgatca
    25561 ccagcgtttt aatccgtttt tcgatctcgg agcctggcat ggtcatctgt tgccagacgg
    25621 cccgaacacc atgggcggct ttccgggcgt tgcgctgctg acggaagagt acatcaactt
    25681 tatggccagc aatttcgacc gcctgaccgt ctggcaggac ggcaagaaag tcgacttcac
    25741 gctggaggca tacagtattc ccggcgcgct ggtgcaaaag ttgatatcga aagatgtaca
    25801 ggtcgaaatg attctgcgct tcgccacgcc gcgcacatca ctactggaaa ccaaaatcac
    25861 cagcgataaa ccgctggatc tggtgtggga tggcgaactg ctggaaaaac tggaagcgaa
    25921 agaagggaaa ccgctttccg ataaaaccat tgctggcgag taccctgact atcagcgcaa
    25981 aatcagcgcc acccgtgatg gcctgaaagt cacctttggc aaagtgcgcg ccacctggga
    26041 tctgctgacc tccggcgaat cggaatatca ggtgcataaa tccctgccgg tgcagactga
    26101 aatcaacggt aatcgcttta ccagtaaggc gcatatcaac ggttcgacga cgctctatac
    26161 cacctactcc catctgctga ccgctcagga agttagcaaa gagcaaatgc agatccgcga
    26221 tattctggcg cgtccggcgt tttatctcac cgcctcgcag caacgctggg aagagtatct
    26281 gaagaaaggg ttaaccaatc cggatgcgac gccggaacag acgcgcgtcg cggtgaaagc
    26341 catcgaaacg ctcaacggta actggcgatc gccgggcggt gcggtgaaat ataacaccgt
    26401 cacgccgtcg gtgaccgggc gctggttctc cggcaatcag acctggccgt gggatacctg
    26461 gaagcaggcg tttgcgatgg cgcatttcaa tccggatatc gccaaagaga atatccgcgc
    26521 ggtcttctcc tggcagatcc agccaggcga cagcgtgcgt ccgcaggatg tgggctttgt
    26581 ccccgacctg atagcgtgga accttagcac cgagcgtggc ggcgatggcg gcaactggaa
    26641 cgaacgtaac accaaaccca gccttgccgc ctggtcggtg atggaagtgt acaacgtcac
    26701 ccaggataaa acctggctgg cagagatgta cccgaaactg gtggcctatc acgactggtg
    26761 gctacgtaac cgcgatcaca acggcaacgg cgtaccggaa tatggcgcaa cccgcgataa
    26821 agcccacaat actgataatg gcgagatgct gtttacagtg aaaaaaggca ataaagaaga
    26881 gacgcagtct gggctgaaca actacgcatg cgtggtggag aaaggccagt acgacagtct
    26941 ggaaattccg gcacaggttg cggcgtcgtg ggaatcgggg cgtgatgatg ccgccgtctt
    27001 tgggtttatc gacaaagaac agttggataa atatgtcgct aacggcggca aacgtagcga
    27061 ctggacggtg aaattcgccg agaaccgcag tcaggacgga acgttgctgg gctactcgct
    27121 attgcaggag tcggtggatc aggccagcta tatgtacagc gataaccatt atctggcgga
    27181 gatggcgacg attctcggta agccggaaga ggccaaacgc tatcgtcagt tagcacaaca
    27241 gctcgcggac tacatcaaca cttgtatgtt cgacccgact acacagttct actatgacgt
    27301 gcgtattgaa gataaaccgc tggcgaacgg ctgcgcgggc aaaccgattg tcgagcgcgg
    27361 taaagggccg gaaggctggt caccgctgtt taacggtgcg gcaacgcagg ccaacgccga
    27421 cgcggtggtg aaggtgatgc tggatccgaa agaattcaac acctttgtcc cgctggggac
    27481 ggcagcgtta accaacccag cctttggcgc agatatctac tggcgcgggc gcgtatgggt
    27541 ggatcagttc tggtttggtc tgaaagggat ggagcgttac ggttatcgcg atgacgccct
    27601 gaaactggct gatacgttct tccggcacgc caaagggtta accgccgatg gcccgattca
    27661 ggagaattac aacccgctca ctggcgcgca gcaaggcgca ccaaatttct cctggagtgc
    27721 cgcgcatttg tatatgttgt ataacgattt tttccgtaag cagtaatttg caacggctgg
    27781 cggatgcggc gtgaacgcct tatccgccct acatgtgcaa tcccccaggc cggataagac
    27841 gcgtcaagcg tcgcatccgg cgttaccacc gggcgtattc tttttgaatc ccatcacaaa
    27901 ccccgcactc cccttttccc ttttctccgg cgacggctaa attagaactc atccgaccac
    27961 ataacaatta ttttacatac tggacaattt tatgagctac ccgtcgctgt tcgccccgct
    28021 ggatttaggt tttaccacgt taaaaaaccg cgtgttgatg ggttcaatgc acaccgggct
    28081 ggaggaatac ccggacggcg ctgaacggct ggcagcgttt tatgccgaac gcgcccgtca
    28141 cggcgtggcg ttgattgtca gcggcggtat cgcaccagat ttaacaggcg ttggcatgga
    28201 aggcggtgca atgctcaatg acgccagcca gatcccacac catcgcacca ttaccgaagc
    28261 cgtacatcag gaaggcggca aaatcgccct gcaaattttg cataccgggc gctacagcta
    28321 ccaaccgcat ctggtcgctc cctccgcatt gcaggccccc atcaaccgtt tcgttcctca
    28381 cgaactcacc catgaagaga tcctgcaact gatcgacgat ttcgcccact gcgcgcaatt
    28441 ggcacgggag gcaggatacg acggtgtaga ggtgatgggg tccgaagggt atttgatcaa
    28501 cgaatttctg acgctgcgca ccaatcagcg tagtgaccag tggggcggcg attaccgcaa
    28561 ccggatgcgg tttgccgtag aagtagtgcg tgcggtgcgc gaacgtgtcg gcaacgactt
    28621 cattattatc taccgactgt cgatgctcga cctggtcgaa ggcggcggta cttttgccga
    28681 aatggtagag ctggcgcagg ccattgaagc ggcgggcgcg accattatca acaccggcat
    28741 tggctggcat gaagcacgta ttccgaccat tgccacgccc gtgccgcgcg gtgcctttag
    28801 ctgggtcacg cgcaaactga aaggccacgt ttcgctgccg ctggtgacca ccaaccggat
    28861 taacgatccg caggttgccg acgatattct ctcgcgcggc gatgccgata tggtatcgat
    28921 ggcgcgaccg tttcttgctg atgcggaact gctgtcaaaa gcgcaatcgg gacgagccga
    28981 tgagatcaac acttgtattg gctgcaacca ggcctgtctc gatcagatct tcgttggcaa
    29041 agtcacctcg tgcctggtga atcctcgcgc ctgccatgaa accaaaatgc caatccttcc
    29101 tgccgtgcag aaaaaaaatc tggcggtggt cggtgcggga cctgctgggc tggcgtttgc
    29161 cattaacgcg gcggcgcgtg ggcatcaggt aacattgttt gatgcgcaca gcgagatcgg
    29221 cgggcagttt aatatcgcca aacagatccc cggcaaagag gagttttacg aaacgctgcg
    29281 ctattaccgc cggatgatcg aagtgacggg tgtgacgcta aaactcaatc acaccgtgac
    29341 agcggatcag ttacaggcgt tcgatgaaac gatcctcgcc agtgggatcg tgccgcgcgt
    29401 tccgcctatc gacgggatcg atcatccgaa ggtattgagt tatctcgatg tactgcgcga
    29461 caaagcgccg gtgggcaaca aagttgccat catcggttgt ggcggaattg gttttgatac
    29521 ggcgatgtat ttaagtcagc cgggcgaatc caccagccag aatatcgccg ggttctgtaa
    29581 tgagtgggga atcgacagta gcctgcaaca ggccggaggt ttgagtccgc aaggcatgca
    29641 aatcccccgt agcccacggc agattgtgat gctccagcgc aaagccagca aaccaggaca
    29701 agggttaggc aaaaccaccg gctggatcca tcgcaccacc ctgctctcac gcggcgtgaa
    29761 aatgatccca ggcgtaagtt accagaagat cgacgatgaa gggctgcatg tggtgatcaa
    29821 cggcgaaccg caaattttgg cggtagatca tgtggtgatc tgcgcggggc aggagccaaa
    29881 ccgcacgctg gcgcaaccgc tcattgatag cgggaaaacc gtgcatttaa ttggcggctg
    29941 cgatgtggct atggagctgg acgcacgacg ggcaattgcc cagggaacac ggctggcgct
    30001 ggagatttaa atcgttgttt gaagcgccgg atgcgacgca tccggcgcgt cgcgttaatc
    30061 aataaacaag gcgggagaaa tgccgaatcg cgttgccagt tttttagcgt gttccagcgt
    30121 taatttcctc ttcccgctca aaacgcgtga caccatcgat ttactgccaa tttccggaag
    30181 atcggaaagg gttaaaccat attgatccat aagggtacga atcacagcta taccgccagg
    30241 catggcttgc gccatggcat taaattcagc aaattcgggc gctgattctt cccacgcggt
    30301 tattttggca cacaccagat ccagcaaggg gttttcagga tcgttgagca gcagatgatc
    30361 taccagttcc agcgcctggg tgtattgttc ctcgttctga atacccgcca gaaaaggtgc
    30421 cacagcagtt aacttttctc ctgcttgcaa gatgtcggca atcgcaatca ttttttcccc
    30481 ttagtacgat gaacggcggt aaagaaatcg tattctttat gcgtcataac ttcacgtatg
    30541 tagcataaac gttgaacaac gctaaacaag agaatgtaga ggatggtgac agggattttt
    30601 tgttttatgg aagcgcgatt ccagaactgg gcagacattg ccggatgcga cgtttgccgc
    30661 gtcttatccg acctgggggt aatgtgctga acgtatgccg gataaggcgt ttacgccgca
    30721 tccggcaggt aacccccaat cttaccgacg acgccccagc ttcactgctt tcagcaccac
    30781 aaatttatta ttcgtggcga tggtggtgca gttgccgaaa atcttcttca gtttatggaa
    30841 gtaatccagg tgacggttgg caacgatata cagctcgccg ttgattttca ggcagcggcg
    30901 ggcatggtgg aacatctccc aggcgacgtt atcggtcagc gcatgttgct gatgaaacgg
    30961 cgggttgcag agcacagcat taaaacggaa aggctccacg ccggagagcg cgttgttgat
    31021 cataaactcg cagcgatcca acgcctcggg catgttggtt tctacgttta accggctgga
    31081 agcaaccgcc atcggcgatt catcgacaaa caccactttc gcctgcgggt ttttatcgag
    31141 caatgtcagg ccaataacgc cattaccgca accgagatcg acaatctcac cttcgagatt
    31201 ctctggcaga tgttgcataa agaagcgcgc gccaatatcc agcccagtgc gggagaaaac
    31261 attcgcatgg ttgtggatag tccagtcagt gccttccagc ttccagctaa cggtctgcgg
    31321 cgcatcggcc aacggcggtt cgttgaaggt gcagttaatc aggcgcgctt tcttccatgc
    31381 cagcgtggtg gtggtcggac cgagcacttt ttcgaacaat tccagcgtgg aagtgtgaat
    31441 gtcacgggct ttggcaccgg caataatgcg cgtatctggc gtgaccactt tgcgcagcgc
    31501 gcgcagttgt tgttccagca atgccagtgt tttcggcact ttgatcagca ccacacccgg
    31561 ctgttgcggg tagtcagcgg tgctgtcgag aaacttcacg ctcgattcat caatcccgtt
    31621 gaggcataaa ttctcgcgcg tggccagttc actgatgtat gagtcgccaa tgctgtacgg
    31681 cttatgttcc gccagcgcac aacttaacgc accaaaggca tcattcagga tcaacaccgg
    31741 gccgcggatt tctgtgtcgt ccaactgttg cagcaaatat tcatccgccg cttcccacgc
    31801 ctgtagcggg ttaacgtcat ccgtcgccgg aaaacgttgt agtgtcagtg aacggaaacc
    31861 gttgtctaag tggctcatcg gccctcctga gtgataaaat ttcgacgttg cccctgaaaa
    31921 gggtgcgtga gtataccctt tttctctttt tcgtgggcgg ttatgagcaa tcttacttat
    31981 cttcagggtt atcccgagca gctactttcc caggtgcgaa cgctgattaa cgaacagcgt
    32041 ctgggcgatg tgctggcaaa acgttatccg ggaacacacg actacgccac cgataaagcc
    32101 ctctggcaat atacccagga tcttaaaaat cagtttctgc gcaatgcccc gccgatcaat
    32161 aaagtgatgt atgacaataa gatccacgtg ctgaaaaacg cgctcggatt acatactgcc
    32221 gtttcgcgtg tgcagggcgg caagctgaaa gcaaaagcag agatccgcgt cgctaccgtg
    32281 tttcgcaacg cgccggaacc gtttttgcgc atgatcgtgg tgcacgagct ggcgcacctg
    32341 aaggagaaag agcataacaa agcgttttac cagttgtgct gtcatatgga accgcagtac
    32401 caccagcttg agttcgacac ccggctgtgg ctaacgcagt tgtcgcttgg gcaggacaaa
    32461 atctgaaaat gatgtcaggc gttatttttt cctcctttag cgcctaaaat cgacctcccc
    32521 cctttcgtta aggataacga ccatgctgcg cgtatttgcc caccttcttc gccgcgtttg
    32581 tttctctcgc cggactctta agattggctg tttattgttg cttatagcag gagcaaccct
    32641 ttttattgcc gatcgcgtta tggtcaacgc cagccaacaa ctgacctgga gcgatgtcaa
    32701 cgccgttccg gcgcggaacg tgggtttatt gctgggggcg aggccgggta atcgctactt
    32761 tacccgacgt attgataccg cagcagagtt gtaccgcgcc ggaaaagtga aatggctatt
    32821 ggtgagcagc gataatggac gtaaaaatta cgatgaagcg tcaggaatgc agcaagcctt
    32881 gatcgctaaa ggcgtaccag caaaagtgat cttctgcgac tatgccggat tctcaacgct
    32941 tgattcggtg gtgcgtgcca aaaaggtgtt tggtgaaaac catatcacca ttatctcgca
    33001 agaatttcat aatcaacgcg ccatctggct ggcaaagcaa tacggcatcg acgctatcgg
    33061 ttttaacgcg ccagacctca atatgaagca cggtttttat actcaactgc gggagaaact
    33121 cgctcgtgtc agcgcggtga ttgacgcaaa aatcctccat cgccagccga aatatctcgg
    33181 gccgttggtt atgattgggc cgtttagtga gcatggctgt ccgacgaaag aataatgcgt
    33241 atctgcgcac gttgaagatg aaaaaggcgt gctacattga cgagagaatc cctttatgga
    33301 gtatccacgc gttatgatac gtttcgctgt gattggtacg aactggatca ctcgccagtt
    33361 cgtcgaggcc gcccatgaga gcggtaaata caagttaacc gccgtttatt cccgcagtct
    33421 tgaacaggcc cagcacttcg ccaatgattt ttctgtcgag catctgttta cgtcgctgga
    33481 agcgatggca gaaagcgatg ccattgacgc ggtgtatatt gccagcccga attccctgca
    33541 cttttcccag acacaacttt tccttagtca caaaattcat gtgatttgcg agaaaccgct
    33601 ggcgtcgaat ctggcggaag tggatgccgc cattgcctgt gcgcgggaaa atcaggtggt
    33661 gctgtttgag gcatttaaaa cggcctacct gccgaacttt catctgttac gccaggcgct
    33721 gccgaaagtc ggcaaactgc gtaaagtctt tttcaactat tgccagtatt cctcgcggta
    33781 tcagcgttac ctggacggag agaatcccaa cacctttaat ccgacattct ctaacggttc
    33841 aattatggat atcggctttt actgcctggc gtcggcggtg gcattattcg gtgaaccgaa
    33901 aagcgtgcag gcaaccgcca gtttgctggc aagcggtgtg gatgcccacg gcgtggtggt
    33961 gatggattac ggtgatttca gcgttacgtt gcagcactca aaagtgagcg attctgtcct
    34021 ggcaagcgag attcagggcg aagcgggatc gctggtgatt gaaaaactgt ctgaatgcca
    34081 gaaagtttgc tttgtaccgc gtggcagcca gatgcaggat ctcacccagc cacagcatat
    34141 taataccatg ctctacgaag cagagctgtt cgctaccctg gtggatgagc atctggtgaa
    34201 tcatccaggg ctggcggtca gtcgtatcac cgccaaactg ctgactgaga tccgccgcca
    34261 gactggggtg atttttccgg cagataacgt aaaactataa ttgccaaagt aaaacagtgt
    34321 aaaaggtatg taacagacca ttgactgact gaatggtctg tcatactttg ttacctgcaa
    34381 aggggagtaa cttcattgtc ggtcgatcgt cattacgatg tgtgaaaaac cacatccggt
    34441 caccgggcaa cccgaaagga atacgcagac gtattccttt tttgttgtaa gtgagacctt
    34501 gccggaaggc gaggtctatg cataaaaagc agcggctgat gtcttccgac gttggccgtt
    34561 tttttatgtg taaggaactt ctatgaatac tgtcggcacg ccgttgctat ggggcggatt
    34621 cgctgttgtt gtcaccatta tgctggctat cgacctgttg ttgcaggggc gtcgtggggc
    34681 acatgccatg accatgaaac aggctgcggc ctggtcgctg gtctgggtga cgctgtcgtt
    34741 actgtttaac gccgctttct ggtggtatct ggtgcaaacc gaaggtcgcg ccgttgccga
    34801 tccacaggca ctggcctttc tcacgggtta tctgattgag aaatcgctgg cggtcgataa
    34861 cgtctttgtc tggctgatgt tgttcagcta tttctctgtt ccggcggcat tacaacgccg
    34921 cgtgctggtg tatggcgtac tcggggcgat tgttctgcgt accatcatga tcttcactgg
    34981 tagctggctg atttcacagt tcgactggat cttgtatatc ttcggtgcct tcctgctgtt
    35041 taccggcgtg aagatggcgc tcgcccatga agatgaatca ggcattggcg acaagccgct
    35101 ggtgcgctgg ctacgcggtc atttgcgcat gaccgacacc atcgacaacg agcatttctt
    35161 tgtgcgtaag aatggtttgt tgtatgccac gccgctgatg ctggtgctga ttctggtgga
    35221 gttgagcgac gtgattttcg ccgtggatag tattccggct atcttcgccg tgaccaccga
    35281 tccgtttatt gtgctgacct caaacctgtt tgcgatcctc ggcctgcgtg cgatgtattt
    35341 cctgctggca ggtgtggcag agcgtttctc gatgctcaaa tatggtctgg cagtgattct
    35401 ggtgtttatc ggtatcaaga tgctgattgt cgacttctac catattccga tcgccgtctc
    35461 gctgggcgtg gtgtttggca ttctggtgat gacgtttatt atcaacgcct gggtgaatta
    35521 tcggcatgat aagctgcggg ttggatgatt tttaatctgc ctaagccgtg taccctgtca
    35581 tcaacatgag caccgttttc tccctctccc caaaaggggc gaggggactg accgagtact
    35641 tttttgacgt tgtcatcagt ctggaagccg cacgttgggc tttattttta tgtcaaataa
    35701 atgtaaccat taagttgcaa aatatgacct ctctttaaaa tccagcattt ttcgcttccc
    35761 gaagctgtaa ctttccttat actcgacctt gcaaacactt tgttacatcc tgaaagatgc
    35821 gtcgacagaa cgcaccaggg atgtgcgaca acacaatgaa aggatcgaaa aatgactacg
    35881 caacgttcac cggggctatt ccggcgtctg gctcatggca gcctggtaaa acaaatcctg
    35941 gccggcctta ttctggggat tcttctggca tggatctcaa aacccgcggc ggaagctgtt
    36001 ggtctgttag gtactttgtt cgtcggcgca ctgaaagccg ttgcccccat cctggtgttg
    36061 atgctggtaa tggcatctat tgctaaccac cagcacgggc agaaaaccaa tatccgccct
    36121 attttgttcc tctatctgct gggcaccttc tctgctgctc tggccgcagt aatcttcagt
    36181 tttgccttcc cttctaccct gcacttgtcc agtagcgcgg gtgatatttc gccgccgtca
    36241 ggcattgtag aagtgatgcg cgggctggta atgagcatgg tttccaaccc cattgacgcg
    36301 ctgctgaaag gtaactatat cgggatcctg gtgtgggcaa ttggcctcgg tttcgcactg
    36361 cgtcacggta acgaaaccac caaaaacctg gtcaacgata tgtcgaatgc cgttaccttt
    36421 atggtgaaac tggtgattca cttcgcaccg atcggtattt ttggtctggt ttcttctacc
    36481 ctggcaacca ccggtttctc cacgctgtgg ggctacgcgc aactgctggt ggtactggtt
    36541 ggctgtatgc tgctggtggc gctggtggtt aacccactgc tggtatggtg gaaaattcgt
    36601 cgtaacccgt tcccgctggt gctgctgtgc ctgcgcgaaa gcggcgtgta tgccttcttc
    36661 acccgcagct ctgcggcgaa cattccggtg aatatggcgc tgtgtgaaaa gctgaatctg
    36721 gatcgcgata cctattccgt ttctattccg ctgggagcca ccatcaatat ggcgggcgca
    36781 gcaatcacca ttaccgtgtt gacgctggct gcggttaata cgctgggtat tccggttgat
    36841 ctgcccacag cgctgctgtt gagcgtagtg gcttctctgt gtgcctgtgg cgcatccggc
    36901 gtggcggggg ggtctctgtt gctgatccca ctggcctgta atatgttcgg tatttcgaac
    36961 gatatcgcca tgcaggtggt tgctgtcggc tttattatcg gcgtattgca ggactcctgt
    37021 gaaaccgcgc tgaactcgtc aactgacgtg ctgttcactg cggcagcttg ccaggcggaa
    37081 gacgatcgtc tggcaaatag cgccctgcgt aactaatact tagccccttt cgtctacggc
    37141 ggaaggggtt ttctccactt taaacggatc aattcccctt ctctgcatac gccagaaacg
    37201 aatgatattc aggccattca tcagcagaaa actgccttca atcatcgtgc cgcctatcga
    37261 ccccgcccag aagttgtgaa tcacccagca acacgttgaa aaccacatta cgcagcgcat
    37321 ggtcagccct ttacagcgga acagcgccca ggtactgaca atcgtgccga ttaccggcaa
    37381 tagttcgaca ggatgatgga acttcgcgag gccaattccg ccagtcagca caataaaaat
    37441 cgccattacc cataagctgc gcgtgcgtaa ggtaatcaat gtacgaatgg cattaaggat
    37501 ggcactggca ccggcaggat aggtgcccag aagaaaaaaa tgtacgccaa taacggcgct
    37561 atagaccgaa agttgctttt tgaagcgacg ttcgtcacga ttgaaaaatg ttgtgatacc
    37621 aatcagaaag gcgatgacac ccacgccctg ggccagccaa tacgcggtca tgataaatcc
    37681 ttagcaggta tggaaaagca aacggcgctt cacattatga aacgccgttt tttattaaca
    37741 actcatttcg actttatagc gttacgccgc ttttgaagat cgccagttcg cggaagtcgt
    37801 tacgctcgtt acaggtttgc ttaccgttgg caaactcaac gatggtgtcg ataaattctt
    37861 ccagcaactg cggcatcgct ttaccgtgga tcagctgacc cgcgtcaaag tcgatccagt
    37921 gttttttctt cgccgccagt tcgctgttgg tggcgatttt caccgtcggt acaaagccgc
    37981 cgtacggcgt gccgcgacca gtactgaaca gcaccatatg gcagcccgca cccgccaggg
    38041 cgctggtcgc tacggcatcg ttacccggcg cacttaacaa gttcagcccc ggcgttttca
    38101 gacgctcgcc gtaacgcagc acgtcaacca cgacactgga acccgctttc tgggtacagc
    38161 caagcgattt atcttccagc gtggtgatac cgcctgcttt gttccccggt gatgggtttt
    38221 cgtagatcgg ctggtcatgg gcaataaagt actgcttgaa gtcattgacc atggtgacca
    38281 gtttttcaaa cgttgcttcg tcgcgacaat ggtccatcag caactgctct gcgccaaaca
    38341 tctccggcac ttcggtcagt acggtagtac cgccgttagc aatcacgtag tcagagaaac
    38401 gccccagcat cgggttagca gtaataccag aaagaccgtc agaaccaccg cactccagac
    38461 caaacttcag ttcgctgagt ttgcccggct cgcgtttatc gttgcgcatc acgctataca
    38521 gctgatgcaa atgcgcgatc ccggcctcga tttcatcatc ctgctgttgg cagatcatga
    38581 aatgaacgcg ttcaggatcg atatcgccca acgtttcacg gaatgcggca acctggttat
    38641 tttcgcaacc cagaccaatc accagcactg cgcccgcgtt cgggtggcgc accatgtttt
    38701 gcagcatggt gcgagtattg atgtgatcgt cgcccagttg tgagcagccg taggtgtggc
    38761 tgaagaggaa cacgccgtcg gtaccttcag cgttgttggt ctctttcagg aaacgatttt
    38821 ggatctgccg cgcgatgccg ttgacacagc ccacggttgg caggatccac agctcattac
    38881 gcaccccgac atcgccgtta gcgcgacgat agatctgcac ttcacgatct gccgcttgcg
    38941 caggcagatc ctgaaaatca ggttgatagc gatactgatc cagatcgctc agattcgtgc
    39001 gcgtattgtg ggcgtgaacg tgttcccccg ccgcaatatc cgccaatgca taaccaatcg
    39061 gcaggccata tttaatgacg ttggcccctt ttgcgatatc cgttaacgca aatttatgtc
    39121 cacgagcaac atcctggcgc agcgtaacag tctggttatc gacactgact tctgtgcctt
    39181 cagccaaatc cgctaaagca accgcgacgt tatccagcgc atggatcttg atgtattgca
    39241 tatcaacccc agaccttagt tcagttcaat ggcgaagtag tcacgcgcat tgttaaagca
    39301 aatatttttc accatctcgc ccagcaggtt gatgtccgcc ggtgcttcgc ccgcttccac
    39361 ccagcgaccg atcatctggc acagaatgcg gcggaagtat tcgtgacggg tgtatgacag
    39421 gaagctacgg ctgtcagtca gcatgccgac aaagcggctc agcagaccga gctgcgccag
    39481 ttgggtcatc tgacgttcca taccgtcttt ctggtcgtta aaccaccagc cggaaccgaa
    39541 ctgcattttg cccggcatac cttcgccctg gaagttaccg atcatggtgc ccagcacttc
    39601 gttatcgcgc gggttcaggc agtagagaat ggttttcggc agcaggtttt cttcattctg
    39661 cttgctcagc agcttagaca gctcttccgc catcggacgg tcgttgatgg agtcaaagcc
    39721 gacatccggc cccagcagtt taaactgacg caggttgtta ttacgcagtg cgccaatgtg
    39781 gtactgctgt acccagccgc gacgtgcata ttcggcacca aggaacacca gcaccgcagt
    39841 tttgaactgt gccacttcgt gctcgctcag ggtttcgcct gccagacggc gcgcgaggat
    39901 actgtccagt tccgcttcgt tcgcttcagc aaacatcacc acatccagcg cgtggtcaga
    39961 cactttacag ccgtgagcgg cgaagtgatc caaacgttta gtcagggcag tttgcaggtc
    40021 agcaaagcgg cgaatgtcgg tatcggaaac ttcgcccagc ttcgccatgt agtcgttaaa
    40081 ggtcgcctgt tcaatgttaa aggctttgtc cggacgccag ctcggcagca ctttaatggt
    40141 gaaagagccg tctttggcga tctctgcgtg atgctccaga gaatcgatcg ggtcatcggt
    40201 ggtgccgacc attttcacgt tcatctgctg catgatgccg cgcgcggaga agttatcctg
    40261 cgccagcagt tcgttacatt cgttccagat ttcatcggca gttgacggag aaagcaattt
    40321 gccagtgata ccaaacggac gacgcagctc gaggtgcgtc cagtggtata acgggttgcc
    40381 aatagtgtgc ggaacagtcg ccgcccaggc gtcaaatttt tcacggtcag acgcatcacc
    40441 ggtgcacaga cgctcggcca caccgttggt acgcatagcg cgccatttgt agtgatcgcc
    40501 cttcaaccag atgtcataca ggtttttaaa acgatagtct tccgcaatct gctgcggcgg
    40561 caaatggcaa tggtaatcga aaatcggctg gtcttttgcg tagtcgtgat acagacggcg
    40621 ggcaaattca gtatctaaca ggaaatcttc agtcataaac ggagtcattt tcgtcttcct
    40681 ctcaacgagt gagctagctt gcttatggtg cgatgctgac aaagttatca caccaatttc
    40741 cagagtccga agatattttc gtgagttaga tcaataaacg tagttaaaaa aattactctc
    40801 aaagtggtaa atctcgctgc aggccgcgcc agcactggcc tcgccgtcgt caggtaatgt
    40861 ccctacaaat attcgcacat ttgtgatggc tctcaccttt taaagttgta tgacaagtta
    40921 tctttctgcc gtcgcaaatc ataagtcgac ggaatgcaaa ttgccgattc attcatttgt
    40981 tagatgaatc gggttaaccg gtacggaagc cgaattagca cgaaactttc atggcaacgt
    41041 tcggggcgtg ccggtttttt ttcggttacc cggtcgtaac taacatcttc agcctctggc
    41101 gggatgatgg ccgcgcttcc tgcggatata acaaaacgat gaggttttac atgcgtaaaa
    41161 ttaaagggtt acgttggtat atgatcgcac tggtgacgct cggcaccgtg cttggttacc
    41221 tgacgcgtaa cactgtggcg gcagctgcgc caactctgat ggaagagtta aacatctcca
    41281 cccaacagta ttcctatatc atcgcagcgt attctgctgc ttatacggtc atgcaaccgg
    41341 tagcgggtta tgtactggat gtgctgggta cgaaaatcgg ttatgcaatg tttgctgtac
    41401 tgtgggccgt gttctgtggt gcaactgcgc tggcaggtag ttggggtggc ctggcggttg
    41461 ctcgtggtgc ggtcggtgcc gcggaagccg cgatgattcc ggcgggtctg aaagccagct
    41521 ccgaatggtt cccggcgaaa gagcgttcca tcgcggtagg ttactttaac gtaggttctt
    41581 cgattggtgc gatgattgcg ccgccgctgg tggtatgggc aatcgtgatg cacagctggc
    41641 agatggcatt tatcatctcc ggtgcgttga gctttatctg ggcgatggca tggctgattt
    41701 tctacaagca tccgcgcgac cagaaacatc tgaccgatga agaacgcgac tacattatta
    41761 atggtcagga agcccagcac caggttgaca cagcgaagaa aatgtccgtt ggtcagatcc
    41821 tgcgtaaccg tcagttctgg ggtatcgcgc tgccgcgatt cctggcagaa ccggcatggg
    41881 gtacttttaa cgcgtggatc ccgctgttca tgtttaaagt ttacggcttt aacctgaaag
    41941 agatcgcgat gtttgcctgg atgccgatgc tgtttgctga cctcggttgt atcctcggtg
    42001 gttacctgcc gccgctgttc cagcgttggt ttggtgtgaa cctgatcgtt tcccgtaaga
    42061 tggtcgtaac gctgggcgct gtgctgatga ttggcccggg tatgatcggt ctgttcacca
    42121 acccgtatgt cgcgattatg ctgctgtgta tcggtggttt tgctcaccag gcactgtctg
    42181 gcgcactgat tacgctctct tctgacgtgt tcggtcgtaa cgaagtggcg acggcgaacg
    42241 gtttaaccgg gatgtccgca tggctggcaa gtacgctgtt tgcactggta gtcggtgcac
    42301 tggctgacac catcggcttc agcccgctgt tcgcagttct ggcagtgttc gacctgttag
    42361 gtgcgctggt tatctggacc gtgttgcaga acaaaccggc gattgaggtg gcgcaggaat
    42421 cacataacga tcccgcaccg caacattaag gcttcgtgta agttttagtt tcccttcaaa
    42481 gccgccttct caggcggctt tttcatcact gcgagtagag ctaaactcgc aaaagtggta
    42541 taacaaatat agtctgccgt atcatgcctg gagcgcatat ggaaatcact gaaccacgcc
    42601 gtttgtatca acaacttgcc gctgatctga aagagcgcat cgaacagggc gtctatctgg
    42661 tgggtgataa actgcctgcc gaacgcttta ttgccgatga aaaaaacgtc agccgcaccg
    42721 tggttcgtga agccatcatc atgctggaag ttgaaggcta tgtcgaagtg cgtaaaggtt
    42781 caggtattca tgtggtttcc aaccagccgc gccatcagca ggcggctgac aataatatgg
    42841 aattcgccaa ttacggtccg tttgagttgc tccaggctcg ccagctcatc gaaagtaata
    42901 ttgccgagtt cgcggcaact caggtcacaa aacaggacat catgaaattg atggccattc
    42961 aggagcaggc acgcggcgaa caatgcttcc gtgattccga gtgggatttg cagttccata
    43021 ttcaggtagc cctggcgacg cagaactccg ccctggcggc tatcgttgaa aaaatgtgga
    43081 cccagcgtag tcataacccg tactggaaaa aactgcacga acacattgat gcccgtaccg
    43141 tcgataactg gtgtgatgac cacgatcaaa tcctcaaggc gctgattcgc aaagatcctc
    43201 atgctgctaa gctggcaatg tggcagcacc tggaaaacac caagatcatg ttatttaacg
    43261 aaaccagcga cgatttcgag ttcaatgccg accgctatct gttcgccgaa aacccggtgg
    43321 tgcatctcga tacagccacc agcggcagta aatgaaattc ctgtccgaca ggcgctacag
    43381 tgcgcctgtt tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc
    43441 tcgcctccct cccctgcatt cagcaaaatc agactccgcg gacatgtaat tttgataacg
    43501 aacaacgttg acctttgtta caattagatt caatttgaat ttatgctttt gaatgctttc
    43561 ttatctcacg atttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac
    43621 ataccaagac agcaataaca ttgcggtagt gcatcattta aaaccagcgt ggcgttaacc
    43681 gattcaccag gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca
    43741 ggattttgaa accctggcca atccgtcgat gattggcata ttgtattttg tcttgtttgt
    43801 cattttgttc cttgaaaacg gcttgcttcc ggcggccttt ttaccgggcg acagtttact
    43861 ggtactggtc ggcgtattga ttgcgaaagg cgcgatgggc tatccgcaaa cgattctgct
    43921 gctgaccgtt gccgccagcc tcggttgctg ggtcagctat attcaggggc gatggctggg
    43981 caatacccgc accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc
    44041 acaccatctt tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcgtt
    44101 tgtcagaaca ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca
    44161 gttcttcaac tggatgagtg gtctgctgtg ggtgttgatc ctgacaactc tgggttacat
    44221 gctcggcaaa acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct
    44281 gctcccggtg gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa
    44341 gaaatatgga aatcgggggt aagggatgca aatacctcgc atgtcacttc gccagctagc
    44401 ctggtccggc gctgttttac ttctggtcag cacactattg ctggcctggt ccgcggttcg
    44461 ccagcaagag tctacgctgg cgattcgtgc cgttcatcaa gggacaacga tgccagacgg
    44521 tttttcaatc tggcatcacc ttgacgctca cggcattcct ttcaaaagta tcacccccaa
    44581 aaacgacact ctgttaatta cctttgactc cagcgaccag agcgccgccg caaaagcggt
    44641 cctcgacaga acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc
    44701 tatgcagtgg ctgacccggt tgcgggataa ctctcatcgc ttcggataac ttcctgttat
    44761 ccgaaacaga tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt
    44821 ggttaacacg accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca
    44881 atggaaggtt caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt
    44941 gccggtagtt atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc
    45001 agctatgccg aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt
    45061 gaagtccggg ccaactgttc agatagccag ctgcgtgccg atcatcagaa gaaaatcgca
    45121 aagcagaaag atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc
    45181 gatgcagata agattgccaa acgcgaacgg aaactggcag aagcgcagga agagctgaaa
    45241 aagctgaaag cgcgcgacta ctaactcaca atagtcacta cttactcacc tggagaaaac
    45301 tatgtcgaaa gaacacacta cggaacatct gcgtgctgag ttgaaatccc tttccgatac
    45361 gctggaagag gtgcttagct catctggcga gaagtcaaaa gaagagttga gtaagattcg
    45421 taacaaagcg gagcaggcac tgaaacagag ccgttatcgc ctgggtgaaa ccggtgatgc
    45481 cattgccaaa caaacccgtg tcgcggcggc gcgtgccgat gagtatgtgc gtgaaaatcc
    45541 gtggacgggc gtgggcattg gcgcagcaat cggtgtagtg ctcggcgttc tgctgtcgcg
    45601 tcgttaatta tggcggacac tcatcacgcg caagggcccg gtaaaagcgt tctgggcatc
    45661 gggcagcgaa ttgtttccat catggttgaa atggtggaga cacgtctgcg gctggcggtg
    45721 gtggagctgg aagaggaaaa agcgaatctc tttcaacttt tactgatgct gggcctgacg
    45781 atgcttttcg ctgcatttgg tcttatgagc ctgatggtgc tagttatctg ggcggttgat
    45841 ccgcaatatc gcctgaatgc gatgattgcc accaccgtgg tgttgctgct actggcactg
    45901 attggcggca tctggacgtt acgtaaatcg cgtaagtcta cgttgctgcg ccacacgcgc
    45961 catgagctgg caaacgatcg gcagctgctc gaggaggagt cccgtgagca gtaaagtcga
    46021 acgtgaacga cgtaaggcgc aactgcttag ccagatccag caacaacggc tggatctttc
    46081 cgccagtcgt cgtgaatggc tggaggcaac aggcgcttac gatcgtcgct ggaatatgct
    46141 gttgagtctg cgctcctggg cgctggttgg cagtagcgtg atggctatct ggacgattcg
    46201 ccatcctaat atgctggtcc gctgggccag acgcggtttt ggtgtatgga gcgcctggcg
    46261 tctggtgaaa acaaccctca agcaacaaca gcttcgcggt taacttcctc tctggccgga
    46321 gaaattccgg ccttatccct caaatttttt gaaaatattt gacagttttc cttgctaaca
    46381 atcacgcttc gtcacgttta tgattctctc catcgacagc aacgacgcta atagcgcgcc
    46441 cttgcacaaa aaaacaatca gcagcctgag cggcgcagtg gagagtatga tgaaaaaatt
    46501 agaagatgtt ggtgtactgg tagcgcgtat tttaatgccg attctgttta ttacggcagg
    46561 ctggggaaaa attactggct acgcgggtac ccaacaatat atggaagcaa tgggcgtccc
    46621 cggctttatg ctgccgctgg tgattctgct tgagtttggt ggtggtctgg caatcctgtt
    46681 cggtttcctg acacgtacca cagccctgtt tactgcgggc tttacgctgc tgacggcatt
    46741 tttatttcac agcaactttg cagaaggcat gaactcgctg atgtttatga aaaacctgac
    46801 tatttctggc ggattcctgc tgctggcaat taccggtcca ggcgcattca gcatcgaccg
    46861 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gagggggaag
    46921 ctcctcgttt ttgctattgg aggatagaaa aatgggtcaa ctgattgacg gcgtctggca
    46981 tgacacctgg tacgatacca aatccaccgg cggtaaattt caacgttcag cttccgcatt
    47041 tcgtaactgg ctcactgccg atggcgcgcc aggccccact ggcaaaggcg gttttgccgc
    47101 agagaaagat cgttatcatc tctatgtttc actcgcctgc ccgtgggcac accgcacgct
    47161 gatcatgcgt aagctcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
    47221 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gcgacacgct
    47281 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
    47341 agtgactgtt cccgtgctgt gggacaaaaa aaaccacacc atcgtcagca acgaatcagc
    47401 agaaatcata cgcatgttca ataccgcgtt tgatgcgctg ggtgcgaaag ctggagatta
    47461 ctacccacca gccctgcaac cgaaaattga cgaacttaac ggctggattt acgacaccgt
    47521 taacaatggc gtgtataaag ccggttttgc caccagccag caagcctacg acgaggcggt
    47581 ggcgaaagtg tttgaatcgc tggcgcgact ggaacagatt ttaggtcagc accgttacct
    47641 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg accacgctgg tgcgttttga
    47701 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
    47761 gtatggcttc ctgcgcgata tctaccagat gccgggaatt gccgaaacag tcaatttcga
    47821 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
    47881 aattggtccg tggcaggatc tcgatgaacc gcatggacga gatgttcgct tcggttaaaa
    47941 aaaggcacct tacgaggtgc ctctgataat tagctaagta cgtgttgttc atgtcggatg
    48001 cggcgtaaac gccttatcaa acctacaaaa catttatatt caatatgttg caatgaccgc
    48061 gcaggccaga taagcgtagc gcatctggca attttgcgct tgcccttatc cttcacagcg
    48121 ccttattaat tcatcgcgat aatatttact gccatttcat tcacgtctat tcttaatttg
    48181 ctgctttaaa aacaagtgat tgagcaaaat tgaggcaaaa atggactggt atctgaaagt
    48241 actaaaaaat tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact ggatgtttat
    48301 tctggtcaac atcatcttta cgttcgtgct ggggctactg gataaaatgt taggctggca
    48361 acgtgccggt ggcgaaggca tcctgacgac aatctacggt attctggtgt ttttaccatg
    48421 gtgggcagtt cagttccgcc gcctgcacga caccgaccgc tcggcgtggt gggcactgct
    48481 attcttaatc ccgttcatcg gctggcttat tatcatcgtc tttaactgcc aggcgggtac
    48541 gccaggcgaa aaccgctttg gaccagatcc gaaactggaa caagaataat aattaagcac
    48601 cttgtaaaag gtgcttaatc gattattttc cgctaaaaag tttgggaatt tcccgcagac
    48661 accaggattt cgcctccccc atactgtcac gacgccaggc cataataata tcgatctcgc
    48721 tggtcgattc cgggctgacg acacgcaacc gcccttccgc aatatctttt tcgaccatcg
    48781 gatacggcat cgtcgccacg ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg
    48841 tgctcaccgt taagcgcggc tgtttgtcca gcaactgtac ggtcaacacc gggcgctcac
    48901 gagcggtatc cgccaccgca attccacgat atttcacgcg cgtcacttca gataacggtt
    48961 ccggctcctg atgaatcggg tgatccggcg cggcaacata gacgttcatt aaggtataga
    49021 gtttgcgcga gttgatctcc gacgaggaac gaaaatgcat atccggcgcg ataacgatat
    49081 ccgcccgccc ctgctccagc cgttcccacg cccccgccag cacttcggtg atgattgcca
    49141 gttgggtatt ggcttttgcc gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta
    49201 ccagcgcctc ggtcacaatg gtgagatgcg tttcccaacc acgcgcgagg gcttccgcat
    49261 cggtagtcag tttatctgcg gcttccagca aaacgcgccc tcgttccagc aacatcctcc
    49321 cgacattggt gaatttggtg cgatggcccg agcggtcaaa cagcaccaca tccagctctt
    49381 cttccagttt ttgcatggtg tagctcagtg cggaaggcac gcgtcccagc tcatccgccg
    49441 ccgccgcaaa actgccccgg cgatcgatcg catccataac ccgtagtgct tccagcgtta
    49501 atgccctttc tttggccatt tcgttctcat tcaggaaatt tgaacatacc gggcagaata
    49561 tctggctaac aatgtagcgt ccagcccctt accataaaag gaagtaaaga gaggtcaaga
    49621 attatgatta ctacccgaac tgccaggcag tgtggacaag cagactacgg atggttgcag
    49681 gcccggtata ctttttcctt tggacactac ttcgacccga aattgttagg ctatgcctcc
    49741 ctgcgcgtgc ttaaccagga agtgctggcc ccaggtgccg cctttcagcc gcgaacctat
    49801 cccaaagtcg atattttaaa tgtgattctg gatggagaag ctgagtatcg cgacagcgaa
    49861 ggcaatcatg ttcaggccag cgccggtgag gtgttgctgc tctctaccca gccgggtgtc
    49921 agctatagcg aacacaatct cagcaaagac aaaccgttaa cgcgaatgca gctttggctg
    49981 gatgcctgcc cgcagcgaga gaatccgctg attcaaaagc tggcgcttaa tatgagcaag
    50041 cagcaattaa tcgcctcgcc agaaggtggg atgggaagcc tgcaattgcg ccagcaagtg
    50101 tggctgcacc atatcgtgct cgacaaaggc gaaagtgcga atttccagtt gcatgggcca
    50161 cgcgcgtatt tgcaatcgat tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa
    50221 gcggcgctaa cctgcggtga tggggcgttt attcgtgacg aggctaacat tacgctggtt
    50281 gccgattccc cactgcgcgc tttgctgata gatttgcctg tctagttgtt tttacaggga
    50341 gatgatgatg agtaaaaaat cggccaaaaa gcgccagcca gtgaagcccg tggtggcgaa
    50401 agaacctgct cgtaccgcca aaaattttgg ctatgaagag atgttgagcg aactggaagc
    50461 catcgtcgcg gatgctgaaa cgcgtttagc cgaggatgaa gccaccgcgt aaagacaggt
    50521 tgccggatgc ggcgtaaacg ccttatccgg cctacaaaac ccaacaacat caacaaattg
    50581 tgcaattcgt aggccagata agacgcgcca gcatcgcatc cggcacaata cccaaaacct
    50641 ggcgtttatc tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc
    50701 gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg cgccacaatc
    50761 ccttcattac cggttacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc
    50821 gaagcactgg tcgaaacctt catcgcgcag ctgttcgatg caccatcgca aatcatgcca
    50881 ctgacatcgc cgatcatact gctgatcgcc atcgagatgg tttcataatg cccatccacc
    50941 agccatgcca tcccggcagc ggcccccatt gctgcggtcg ttgcggcaca cagcgcagac
    51001 aaacgcggta actggttatg gatgtaaatc gcgctcaaat gcgaaagcat cagcgcacgc
    51061 gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacggg cattgttgcg
    51121 gtgatcccct ggttacccga gccggagtta ctcattgccg gaagcgtagc gccgcccata
    51181 cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc
    51241 aaaccgcgcg cgcactgttt ttccagcgtc gcaccaatat gcagccccca gttaccgctc
    51301 aaaccttcct gcgataacgc acaatttaac ttcgcagaat cgagaataaa gcggatcgcc
    51361 gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgtcct ggaaagcacc
    51421 gtcagcggcg actcttgctc gccctctgtc acacacgcct gctgggtaaa caccacacca
    51481 ttgtgcgtct caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac
    51541 ttctcaccgt tccagacttt ggcgcgtgag aagaggattt catcgcaagg ttcctggatc
    51601 ttaactgaga ctttccccgc cgccagcagt gccttggcat cggaaattgc ctgcgcagtt
    51661 gcgtctttca gcacttccag cccggcgtta gcatttccac ctaacgctcc cagcgccgcc
    51721 gcaattggca gccccaccat tcctgtaccg ggaacggtga cacccagacc gttcttcatc
    51781 agatttggag aaacccaggc ttctacacgt tcaaccggac cttccagttc agccgcagca
    51841 accgccgccg ccagcgccag tgaaatcggt tcagtacacc ccagcgccgg ttttacttcc
    51901 tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta
    51961 aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac acaaagcaac
    52021 aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact
    52081 ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata
    52141 gaggtgaagc tcaacaccgg ggcgttcagt acgatggcgc tccaggccag caaaatggcg
    52201 aaaatcatga tgccgcgctg aacgagattt tcgttaatct tctcagcagg tatcttgcga
    52261 cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag
    52321 aatgccgtca ttacagcaaa gatattgaga atgacgctga ctactttcac ccacgctgcg
    52381 ccatcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct
    52441 tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggta
    52501 acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgccacttca
    52561 attgattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg gataaacaga
    52621 atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc
    52681 ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc cagcgccgcg
    52741 accaccagca gcttggtcag caccatcccc gtcgaaattt tgaataacaa tttctcgccg
    52801 cgtgaggaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac
    52861 agcaaccctt ccgtcacgcc gaaggtatgc aggtaggaag cactatcgtt ggtgatggcg
    52921 gtggaataga cgaacatcca aatcaccagc attacgaaat agagcgcacc taacaggatg
    52981 ccccagtttt tacctaaata accgctaatg acactcgggt aatctttaca ttctggtgat
    53041 tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc
    53101 gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc
    53161 cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat
    53221 ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg
    53281 tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaat attttgtagg
    53341 gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga
    53401 gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt
    53461 tgaaataacg gagatggata agaattttct actaaattaa ccacaagaga atattcattc
    53521 tctatttgcg gcagatcaca aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct
    53581 gacagcaatg tcagccacag accactttaa tggccagtcc tccgcgtgat gtttcgcggt
    53641 atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa
    53701 cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat
    53761 taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca
    53821 gcccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc gggctgccgc
    53881 ccagtagttc agttaaccct gccgccgcca ttgaacaggc cacgccaatc tccccctgac
    53941 agccgacttc cgcgccagag atggaggcgt tcattttata cagcgcgcca atagccccgg
    54001 ctgcaagaaa atagcgggca attgaccgct catttaccgg acgacggaac ttatcgtaat
    54061 aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc
    54121 cgccagctgc gttttcttca ctaaccgcca gcgcgtacat gttgatccag tcgatgacat
    54181 tcatcggatc gttagagatg ttatcgctgg aaaccagttg acgacgcagc gctacggcac
    54241 ggcgcggcac attgagcgga ccaggcagca cgccttcagt attcattcca cgttcaatac
    54301 cgtcatgcat cacttgccag atacgggcaa accctgcgtc aatttccgct ttactgcgca
    54361 gcgccagctc gttgtgcatc atcaggccag atatagacag gccgttgtaa tcacacattt
    54421 tcagcagttc acctgctgag tggaaatcgt aaggtacggg tgtttcgaca tcgtgcgaca
    54481 ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttgctta
    54541 atagttcttc ctgtcccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca
    54601 tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc gcgccagaag
    54661 ccaccggcaa ccgcccgctg cgcgtcacta actctataaa tgcagggatc tcatcaatga
    54721 caacatcctg cggactgttt cctgccagtc ccatgatgat ggcgacatcc gtggcatggc
    54781 ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta
    54841 ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacag
    54901 tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca
    54961 caccctcgga ttgccgttca gtaaagtgga gcggaacgac cttgcgaccg tcccgctcac
    55021 gaggccttac gcactacgta ctgcgatggc ttcaatttcc aacttcacat ctttcggcaa
    55081 acgcgcgacc tgcacacagc tccgggtcgg gtaggtcgcc tgatgttcat cgaagaactg
    55141 cttatagact tcattgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt
    55201 gatgatatcg cccacactca gcccggcagc aaccacgatc gctttgacgt tttcgaggct
    55261 taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtctgtgggc aaaccggaat
    55321 ttgcccggag gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc
    55381 gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct
    55441 gggtaaaggt acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg ttaaagcgca
    55501 cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat
    55561 ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg
    55621 cttcgtggtg gaaataccca tccagcaggc cgacaaggtt cgttttacgt atcggatctt
    55681 ctttgcccag ggccgcaggg acgatggaga aggtgtacga gatcccatct ttggcgtagg
    55741 tgaacggcag tttcgccacc gacgtcaatg aggccacggc gcctttgcga tcacgaccgt
    55801 gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg gcgaccgtcc ggcgtgttcc
    55861 cggttttctg accgtacacc acgttagagg tgatagtcag aatcgattgg gtcggcacag
    55921 cattgcgata ggttggcaaa gctttaattt tcttcataaa gcgttcaacc agatcgcagg
    55981 caatgctgtc tacgcgctcg tcgttgttgc catactgcgg atattcgccg tcaatctcga
    56041 aatccaccgc caggccgttt tcatcacgga ttggtttcac acgagcatat ttgatggcag
    56101 acagggagtc cgttgccacc gacaggcccg cgatgccgca cgccatggtg cgatagacat
    56161 cacgatcatg tagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa
    56221 tgatattcag cgcgctgatg tactgtaccg ccagccagtc catgaagtgg tcgaggctat
    56281 ccatcacttt gtcgtagtcc agcacgtcat ccatcaacgg agccgttttc gggccaacct
    56341 gaatcttcag cttctcgtcc attccgccgt tgattgcgta cagtagcgtt ttcgccaggt
    56401 tagcgcgtgc tccaaagaac tgcatttgct taccaatcac cattgggctg acgcaacagg
    56461 caatcgcgta atcgtcgcta ttgaagtcag tacgcatcag atcgtcgttt tcatactgca
    56521 aagaggatgt gacgatagac acctgcgcgg catatttttt gaaggcaatc ggcaaggctt
    56581 ccgaccacag aatagtcagg ttaggttccg gtgcaggccc catagtgtgc agggtgtgca
    56641 aatagcggaa ggagtttttg gtcaccaggg ttcgaccgtc cagccccatc ccgccgatca
    56701 cttccgtcgc ccagatcggg tcgccggaga acagcgaatc aaattccggc gtacgcaaga
    56761 agcgcaccat acggatcttc atgatgaagt ggtcgatcag ttcctgtgcc tgctgctcat
    56821 tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc gaggaacgat gccgtgcgcc
    56881 ccagcgacat cgcgccgcca ttttgcgatt tcactgccgc caggtaagcg aagtagagcc
    56941 actgcaccgc ttcctgtgca ttctgcgccg gacgggagat gtcaaaaccg tatttcgccg
    57001 ccatttcttg aatctgcaac agcgcacgac gatgttccgc cagctcctca cgcagacgga
    57061 tcgtagcttc gagatcttcg cctttttcca gacgggactg gagatcggca aactgcagtt
    57121 cgcgttcacg taccagataa ctgatgccgt aaagcgccac gcggcggtag tcaccgatga
    57181 tacgcccacg accataacca tctggtaaac cggtcagtac accagattta cggcagcgca
    57241 gcatatccgg tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa
    57301 agatgtattc aaattcgcta tccatttcac gaccatacgc gtggaatgaa cttctaatca
    57361 tattaatgcc gccgaacgga tgcagcgcac gctttaatgg cgcatccgtt tgcagaccca
    57421 ctattttttc cagcggctgg tcaatatatc ctgcatcatg agcggtaatt gtggtggcaa
    57481 tattggtatc gaaatccaat ggcgcgtgag tcgcattttc gatacggatg ccttccatga
    57541 ctttttccca caattccgtg gtggcaggcg tcgcttcggc gagaaaagat tcatcgcctt
    57601 cataaggtgt atagttatgt tgaataaaat cgcggacatt aatttcgttt ttccagtccg
    57661 tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca atatctacct
    57721 tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct gcgggcgcgt
    57781 tcactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgtta gtcggaatta
    57841 cggcgcaaat gacacgcgca ttttcactgg aaacaattcg ctctccaaaa gagttagagc
    57901 gattattcat ttctgtatca atttctacgc ctaatacagc taaatgttcc atgaccagac
    57961 gacgaattaa acttgaattc tctcctattc cgccggtgaa tataattcca tccaggcgat
    58021 gtaatgaagc agcgtgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg
    58081 ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag
    58141 aaaggccgga aatacctaat aatcccgact ctttattcac tacgcgttcc aggtccccca
    58201 ggctctggtt ggtttggctg gcgacccagg acatcgcccc gaagtcgaca tcaccactgc
    58261 gggtacccat catcaagcct tccagcggcg tcattcccat ggaagtgtca acactctgac
    58321 cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt
    58381 catcttccgc cagattcagc agagaatgcg cgcgctggga aacatagcgg tgcgacgtgc
    58441 cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc aggccgtata
    58501 aataagcttc cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta
    58561 cgcccgggaa taattgctgc gccgattcaa taccacttaa attggcgtaa ttatgcaggg
    58621 gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata atggcggact
    58681 cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaggccaca ttgtcattta
    58741 aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag
    58801 ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt
    58861 cggcaatacc tgacattaat acttcacagt cgctggcatt gagtacggaa aacttaatcg
    58921 aagacgaacc acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc
    58981 tgagttacgg attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg
    59041 gtaacaaaca cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggatg
    59101 gcatacatcg gcaacaggca cagcagtgat gcgataatcg gtgcgcccat ggcttcaatc
    59161 aggtcgagga tgttcgggtt ggcgtaggca acaacccagg tggagcccat aatgaaaatc
    59221 atgctgatag tattcagttt acccagcgac actttggttt tgtcaccttt ataaccgaac
    59281 ttcagaatca aaccattcag cccttccagc gtccccagat agtgaccgaa gaaagatttg
    59341 aaaatagcca cgagtgcgat gatggaagcg gcatattcca gtgtaatcgc aaacgttgtt
    59401 ttggtaccgg tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc
    59461 gctttcgctt cagccatatt cgccggagac agcgtaaaca ggcagctaaa ggcaaagaac
    59521 atcaccactg caaccatcag catactggcg cgagagatga tttgtgaaca tttacgctcg
    59581 gtgaagtcgc gaccaaaatc tttctcgtac tcttcacgtt tagaaaccac gaaggaagag
    59641 acgattggcg agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacggtgatc
    59701 aggataccgt catgaccagt taacgacagc gaaccgaggt caacctggtc gataactgca
    59761 gagttccagt aagggatcag cgaaagagaa atcagcacca ggctggcgat aaacggccat
    59821 accagatagc tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc
    59881 agcagcaaca ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc gagctggttt
    59941 tcccagaacg tcataaaggt attggtaata gtaacgccat aaatccacaa cagtgggcaa
    60001 atcgcgaaga agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc
    60061 accgtttccg taatgttgcc ggaagggtta gagccagaaa gacacagacg cgccagtgcc
    60121 cggtggcaat aaaacgcgat ggggtatgct aataccaaca tcagaagaat cgggatcagt
    60181 ccgccaaaac ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac
    60241 aagcctaacg tccatgtggt atctgattta cgccaggacg attgttttgt ctggctggat
    60301 acaatgctat ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa
    60361 ccggtgattt gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt
    60421 tttctgtttt gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg
    60481 cctgcgcctt cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt
    60541 tcgtcttcgc tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa
    60601 ttacccgggc gggagacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtagtt
    60661 atttctccgg agtggaaaga agccgccatg ccgtgaacgt tttcagactg tacgccaata
    60721 acacggatgg tcgggttgat agatttaatt gccaccgcaa tacctgcaat taaaccgccg
    60781 ccgccaattg gcacaatcac gttatcgaca tcatagagat cttccataat ttccagacca
    60841 atcgttccct ggccagcaat cactttcgga tcatcgtaag gtgggataaa aatacggcct
    60901 tccatttcga caatttcgct cactttagca atagtatcgt tgaagttatc accatgcaaa
    60961 acgacttctg cggagtagtc gcacgttgcc gcgactttgg atttcggcgc accttttggc
    61021 atcaccactt taccgtcaat acccagcatc gcgcaggaga gcgaaacccc ttgcgcatgg
    61081 ttgcccgcag agcaggccac tacaccttta cgtttttccg catcagtcag tgaacttaat
    61141 ttattaaatg cgccacgaat tttaaatgaa ccggtacgct gcatgttttc gaatttgagg
    61201 aatatttcac ctttgcaacg ttcactaaaa tagttggagc gaggcatacc tgttttataa
    61261 attcgcccag ccagtcgttg ttttgcttca atgatgtcat caatagcaac cgggagatcg
    61321 tatgtaatat gcattataaa acctcttagc ctgaattaaa tatgtaggta accgtaaccg
    61381 acacctgcaa aacagacagg tggattattt atgtttaaat taaaacaaat aactaatcaa
    61441 cttcaattaa ttgccttcgt ctacacccat tataagatga atactctttg gctaattcca
    61501 ccaaaaccga tgctgctttt ttaatacgat aatttttcga ccataccgcg gcatattgtg
    61561 ccacaggtaa tgtttcttca accggaatag taataaattg attagaacca aaaggtgatg
    61621 tcatatcaca aggaattaca gttaagaaat cagcattgag aacaagatta taaattgtca
    61681 cgactgagtc ggttttaacg atgttttcaa tactgatgcc atttctttgt aacgtagtaa
    61741 gtagttcgct gtagtacccc atattggttt gtggcaacac ccactgttcg ttcttcaacg
    61801 actccagcgt ggtggtgccg gtgcatgttc gggacttact ggctaccagc acaaactcgg
    61861 actcgaacag cggctcaaca tgtaaatcct gaagcttcat ttctgcgctt aacgtaccaa
    61921 tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa cgaagacagt tgcgcttcat
    61981 acatagaaac ctgcgctttc gggaacacct ctttgaactt gttgatcatc cctgacataa
    62041 aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc caccaccgcc tcagaagaca
    62101 taccgcttat ctcattaacc atatttttca tttcacgggt aatggattcg gaacgggaga
    62161 gtaacaattg accggcaggt gttaatgtta caccggtatt tttccgcacc actaactcca
    62221 caccaaaata atcctcaata tcgttaatga ttttactgac ggccggttga gttaacccta
    62281 actcttttgc agccgagccg atagaaccac ttctaataac ttcctgaaag actaccaggt
    62341 gctgcgtttt cggaagaaga atagtgctca taacgaccta cgttaattac ctcattgacg
    62401 gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg accactcaca aattaacttt
    62461 caatactttc catggcatcg ttaccccaac caaatacctt taaatatcaa caagttaaaa
    62521 atatgaaaat cagcataaac cctgattttt gtcaaaaaaa atgacgggga taaccatatt
    62581 ttatggcgat aacatcattc tttatgtggt taatacaaaa aaggggctga gactatattt
    62641 caagcaatta ccggataatt accaggcaaa atttgagtta aatttaatga gtaagttctc
    62701 aaattttatt ataaacaaac catt
//
LOCUS       DADTFG010000007        61146 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.007, whole genome shotgun sequence.
ACCESSION   DADTFG010000007 DADTFG010000000
VERSION     DADTFG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.007"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(41..1009)
                     /gene="astE"
                     /locus_tag="J1138_000468"
     CDS             complement(41..1009)
                     /gene="astE"
                     /locus_tag="J1138_000468"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBA8452313.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQDPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1002..2345)
                     /gene="astB"
                     /locus_tag="J1138_000469"
     CDS             complement(1002..2345)
                     /gene="astB"
                     /locus_tag="J1138_000469"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBA8452314.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHCFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGIQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRSRVNGFMAIEVPAAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWRY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(2342..3820)
                     /gene="astD"
                     /locus_tag="J1138_000470"
     CDS             complement(2342..3820)
                     /gene="astD"
                     /locus_tag="J1138_000470"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBA8452315.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGDAVMRLWQQAGLPPGVLNLVQGGRATGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLFLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGLPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(3817..4851)
                     /gene="astA"
                     /locus_tag="J1138_000471"
     CDS             complement(3817..4851)
                     /gene="astA"
                     /locus_tag="J1138_000471"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBA8452316.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(4848..6068)
                     /gene="astC"
                     /locus_tag="J1138_000472"
     CDS             complement(4848..6068)
                     /gene="astC"
                     /locus_tag="J1138_000472"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBA8452317.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYACMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAVKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            6514..7320
                     /gene="xthA"
                     /locus_tag="J1138_000473"
     CDS             6514..7320
                     /gene="xthA"
                     /locus_tag="J1138_000473"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBA8452318.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7487..8197
                     /locus_tag="J1138_000474"
     CDS             7487..8197
                     /locus_tag="J1138_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA8452319.1"
                     /translation="MNAERKFLLACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            8202..8879
                     /gene="ydjY"
                     /locus_tag="J1138_000475"
     CDS             8202..8879
                     /gene="ydjY"
                     /locus_tag="J1138_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBA8452320.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            8893..9600
                     /locus_tag="J1138_000476"
     CDS             8893..9600
                     /locus_tag="J1138_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA8452321.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            9600..10148
                     /locus_tag="J1138_000477"
     CDS             9600..10148
                     /locus_tag="J1138_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBA8452322.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10158..11324
                     /locus_tag="J1138_000478"
     CDS             10158..11324
                     /locus_tag="J1138_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA8452323.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQTLLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            11297..12832
                     /locus_tag="J1138_000479"
     CDS             11297..12832
                     /locus_tag="J1138_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8452324.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLIFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMVLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWQEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            12832..13485
                     /locus_tag="J1138_000480"
     CDS             12832..13485
                     /locus_tag="J1138_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA8452325.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            13552..14859
                     /locus_tag="J1138_000481"
     CDS             13552..14859
                     /locus_tag="J1138_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBA8452326.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(14868..15488)
                     /locus_tag="J1138_000482"
     CDS             complement(14868..15488)
                     /locus_tag="J1138_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBA8452327.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALVGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            15575..15982
                     /gene="nudG"
                     /locus_tag="J1138_000483"
     CDS             15575..15982
                     /gene="nudG"
                     /locus_tag="J1138_000483"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBA8452328.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            complement(15948..16220)
                     /locus_tag="J1138_000484"
     CDS             complement(15948..16220)
                     /locus_tag="J1138_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBA8452329.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            16456..17799
                     /gene="gdhA"
                     /locus_tag="J1138_000485"
     CDS             16456..17799
                     /gene="gdhA"
                     /locus_tag="J1138_000485"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBA8452330.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(17876..19831)
                     /locus_tag="J1138_000486"
     CDS             complement(17876..19831)
                     /locus_tag="J1138_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBA8452331.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGRE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(19836..20879)
                     /gene="selD"
                     /locus_tag="J1138_000487"
     CDS             complement(19836..20879)
                     /gene="selD"
                     /locus_tag="J1138_000487"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBA8452332.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(20996..21547)
                     /locus_tag="J1138_000488"
     CDS             complement(20996..21547)
                     /locus_tag="J1138_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBA8452333.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAVGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            21708..23564
                     /gene="sppA"
                     /locus_tag="J1138_000489"
     CDS             21708..23564
                     /gene="sppA"
                     /locus_tag="J1138_000489"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBA8452334.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SIRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23731..24747
                     /gene="ansA"
                     /locus_tag="J1138_000490"
     CDS             23731..24747
                     /gene="ansA"
                     /locus_tag="J1138_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBA8452335.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPVPHGEGALIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            24758..25399
                     /gene="pncA"
                     /locus_tag="J1138_000491"
     CDS             24758..25399
                     /gene="pncA"
                     /locus_tag="J1138_000491"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBA8452336.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDIAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(25492..26850)
                     /locus_tag="J1138_000492"
     CDS             complement(25492..26850)
                     /locus_tag="J1138_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452337.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(26968..27726)
                     /locus_tag="J1138_000493"
     CDS             complement(26968..27726)
                     /locus_tag="J1138_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8452338.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(27863..28843)
                     /gene="ydjG"
                     /locus_tag="J1138_000494"
     CDS             complement(27863..28843)
                     /gene="ydjG"
                     /locus_tag="J1138_000494"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBA8452339.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(28853..29800)
                     /locus_tag="J1138_000495"
     CDS             complement(28853..29800)
                     /locus_tag="J1138_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBA8452340.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KARQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            complement(29805..30641)
                     /locus_tag="J1138_000496"
     CDS             complement(29805..30641)
                     /locus_tag="J1138_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBA8452341.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFGSDGK
                     AE"
     gene            complement(30662..31705)
                     /locus_tag="J1138_000497"
     CDS             complement(30662..31705)
                     /locus_tag="J1138_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HBA8452342.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGGKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(31722..33101)
                     /locus_tag="J1138_000498"
     CDS             complement(31722..33101)
                     /locus_tag="J1138_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452343.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(33128..34204)
                     /locus_tag="J1138_000499"
     CDS             complement(33128..34204)
                     /locus_tag="J1138_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA8452344.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(34574..34846)
                     /locus_tag="J1138_000500"
     CDS             complement(34574..34846)
                     /locus_tag="J1138_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBA8452345.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(34888..35301)
                     /gene="msrB"
                     /locus_tag="J1138_000501"
     CDS             complement(34888..35301)
                     /gene="msrB"
                     /locus_tag="J1138_000501"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBA8452346.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            35643..36638
                     /gene="gapA"
                     /locus_tag="J1138_000502"
     CDS             35643..36638
                     /gene="gapA"
                     /locus_tag="J1138_000502"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBA8452347.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            36722..37606
                     /locus_tag="J1138_000503"
     CDS             36722..37606
                     /locus_tag="J1138_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBA8452348.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEILWLSNNTPFKNGVAIRGGIPLCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVVLTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(37657..38511)
                     /gene="yeaE"
                     /locus_tag="J1138_000504"
     CDS             complement(37657..38511)
                     /gene="yeaE"
                     /locus_tag="J1138_000504"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBA8452349.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLITQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(38601..39347)
                     /locus_tag="J1138_000505"
     CDS             complement(38601..39347)
                     /locus_tag="J1138_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HBA8452350.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            39783..41717
                     /gene="yeaG"
                     /locus_tag="J1138_000506"
     CDS             39783..41717
                     /gene="yeaG"
                     /locus_tag="J1138_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBA8452351.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            41830..43113
                     /locus_tag="J1138_000507"
     CDS             41830..43113
                     /locus_tag="J1138_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBA8452352.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            43260..44735
                     /locus_tag="J1138_000508"
     CDS             43260..44735
                     /locus_tag="J1138_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBA8452353.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            44916..46406
                     /locus_tag="J1138_000509"
     CDS             44916..46406
                     /locus_tag="J1138_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBA8452354.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            46449..46952
                     /gene="yeaK"
                     /locus_tag="J1138_000510"
     CDS             46449..46952
                     /gene="yeaK"
                     /locus_tag="J1138_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBA8452355.1"
                     /translation="MTEMAKESVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(46953..47057)
                     /locus_tag="J1138_000511"
     CDS             complement(46953..47057)
                     /locus_tag="J1138_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452356.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            47227..47673
                     /locus_tag="J1138_000512"
     CDS             47227..47673
                     /locus_tag="J1138_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBA8452357.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(47630..48451)
                     /locus_tag="J1138_000513"
     CDS             complement(47630..48451)
                     /locus_tag="J1138_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8452358.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            48548..49729
                     /gene="nimT"
                     /locus_tag="J1138_000514"
     CDS             48548..49729
                     /gene="nimT"
                     /locus_tag="J1138_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBA8452359.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGILRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLLGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            49784..50131
                     /locus_tag="J1138_000515"
     CDS             49784..50131
                     /locus_tag="J1138_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBA8452360.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(50153..50407)
                     /gene="yoaF"
                     /locus_tag="J1138_000516"
     CDS             complement(50153..50407)
                     /gene="yoaF"
                     /locus_tag="J1138_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBA8452361.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            50590..51615
                     /gene="dgcP"
                     /locus_tag="J1138_000517"
     CDS             50590..51615
                     /gene="dgcP"
                     /locus_tag="J1138_000517"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBA8452362.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCVAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            51648..51746
                     /gene="yoaK"
                     /locus_tag="J1138_000518"
     CDS             51648..51746
                     /gene="yoaK"
                     /locus_tag="J1138_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBA8452363.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            51749..51823
                     /gene="yoaJ"
                     /locus_tag="J1138_000519"
     CDS             51749..51823
                     /gene="yoaJ"
                     /locus_tag="J1138_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBA8452364.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(51883..52131)
                     /locus_tag="J1138_000520"
     CDS             complement(51883..52131)
                     /locus_tag="J1138_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA8452365.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(52279..52461)
                     /locus_tag="J1138_000521"
     CDS             complement(52279..52461)
                     /locus_tag="J1138_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBA8452366.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(52465..52824)
                     /gene="yeaR"
                     /locus_tag="J1138_000522"
     CDS             complement(52465..52824)
                     /gene="yeaR"
                     /locus_tag="J1138_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBA8452367.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(52997..53635)
                     /gene="leuE"
                     /locus_tag="J1138_000523"
     CDS             complement(52997..53635)
                     /gene="leuE"
                     /locus_tag="J1138_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBA8452368.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(53762..53929)
                     /locus_tag="J1138_000524"
                     /pseudo
     CDS             complement(53762..53929)
                     /locus_tag="J1138_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            53918..54088
                     /locus_tag="J1138_000525"
     CDS             53918..54088
                     /locus_tag="J1138_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBA8452369.1"
                     /translation="MTTGGKQPGDIDFYVVRKNTEGEYSSPGGRVNEGTENEVVIQES
                     VFTRRGVERILR"
     gene            complement(54178..55293)
                     /gene="rnd"
                     /locus_tag="J1138_000526"
     CDS             complement(54178..55293)
                     /gene="rnd"
                     /locus_tag="J1138_000526"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBA8452370.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(55375..57060)
                     /gene="fadD"
                     /locus_tag="J1138_000527"
     CDS             complement(55375..57060)
                     /gene="fadD"
                     /locus_tag="J1138_000527"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBA8452371.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(57265..57846)
                     /gene="yeaY"
                     /locus_tag="J1138_000528"
     CDS             complement(57265..57846)
                     /gene="yeaY"
                     /locus_tag="J1138_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBA8452372.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(57886..58581)
                     /gene="tsaB"
                     /locus_tag="J1138_000529"
     CDS             complement(57886..58581)
                     /gene="tsaB"
                     /locus_tag="J1138_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBA8452373.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLLDGEVLLPAAEDMLPIACQMFAEGKMVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(58639..60549)
                     /locus_tag="J1138_000530"
     CDS             complement(58639..60549)
                     /locus_tag="J1138_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBA8452374.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIELAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            60681..61025
                     /locus_tag="J1138_000531"
     CDS             60681..61025
                     /locus_tag="J1138_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBA8452375.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFDQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT        15194 a        15892 c        14808 g        15252 t
ORIGIN      
        1 agcaacttaa tacccgcaga atgatttctg cgggtaagta ttagcttatt ttttcgagca
       61 ttaatcccgc acgtaatccc aacgctacca acggattagg gaataacaca tactctacat
      121 catgggttac ggtaaaacgt tcctccccgt cctgcgccag caatgttcct ttctcaaacg
      181 gcataaaatt cagcgtgtca tttgccatat gcatttcgaa ggacggcgag tggcgagtaa
      241 tttgcgaaac cacccgataa cggagcggcg gtgttctcac gataccgaca ctctcaccag
      301 atagcagcgc agcaattgcg ctggcagtta ctgcaaactg gcgaagatcg ttttgcccaa
      361 agggcaacgc tttgccaagt tccagcgtac aggccagcgc gccaaaatgt ctggcgctga
      421 aatgggtaaa cgtaccacca gggtcctgat ggaacaccag cgcctccagc cccgccgcac
      481 ccagccacgt cagaaatttc tcgtcccagg gaatgtcgcg ttgcggtaat acaccgaact
      541 gcggatgcaa ggagccacga attgcggtat gtagatcaag gtgccagcgc acagattctt
      601 tgccctggtc ataaaaatct tccaggcact gctccagttc gcgcgcccga caggtttccc
      661 cgctttcagc aaacagttgc caacgaccgc caaacattcg attcatatcg ctatggcaat
      721 aacgtttccc ttgcttcagc gcaggaggat tcccgaggat caccagtaac cgccaacgta
      781 acgggatctc accgtgagat atcgcgccaa gcaacgcgtc cagcatttcc acaggagccg
      841 tctcattacc gtggatcccc gctgaaatca ccagcgcgcc ttgcggtggc gttaatggcg
      901 tcagttccag cacaccatcg cccagccagc gccagcgaac gccgttgatt tctcgctcgg
      961 tgataaccgg ttttttaccc gttaaggtta gagcaagaaa attatccatt gccccctccc
     1021 tcgcgctgga atggataaac cgaaccaaga tccaataatt gagtcagtgt atccagcgct
     1081 tcccttcctt cgcgcagcaa ttgtggatcg gcaagatccg ccgcagtaag acgatcgcgg
     1141 tagtaacgat ccacccagtc attgagcgca ttaaacagcg tatcgttcat catcaccgcc
     1201 ggattcaccg cccggcgttc ttcttctgtc aacaccacgc gcaaccgcag gcacgccggg
     1261 ccaccgccat tcgccatgct ttcacgtaaa tcaaaaactt ttagtgagct tattgggtta
     1321 tcggcagcaa gcaattcatt gagataacgc catactccag catgttcccg acattcctga
     1381 ggcagcacca acatcatgga accatcatcg cggctcagta actggctgtt aaacagatac
     1441 gtagacaccg catccgacac ggaaacctgt gccgccggaa cttctatcgc cataaaacca
     1501 ttgacccgcg aacgcaggtt tgccagtaac tgtgactggc gagcgaacgc ctgttgatgg
     1561 caaaacagca cctggcggtt actcacggca atcacgtcat tatgaaaaac gccctggtcg
     1621 ataacgtccg ggttttgctg ggcgaaaatc acctgttggg gattcacctg attcaacctt
     1681 gccaccgcct cgctggcttc ccgagtctgc cgcgccggat aacgggaagg ccgggtatca
     1741 ttgccctctt ctcgcccgta gacaaaaagt tgaatacccg gttcaccgta atgaccgccg
     1801 agacgattgt ggtttgccgc tccctcatca ccgagcaagg ctacctgcgg caacgccgaa
     1861 tggacactaa atttctcttc gtcgttaaaa atcgctttta acaacgattc ggtgacgggg
     1921 gcttccagcg aacggtgaaa tttattgttc aggttggcaa cggtgagatg cactttgcca
     1981 tccagcgtat cggcagatgg cgcgatcgtt gccgcattgg ctacccacat tggcgaagcg
     2041 gagctgacgc tggaaagcca gtgcggtgcc tggcgggcaa ccttttccag cacttgttca
     2101 tcgctaccac tgaatcccaa ctgacgtaaa accggtataa acggacgctc gtgcggcggg
     2161 atcacggcct gggggaatcc cgcatcggca agggttttca ttttcagtaa gccctgtttc
     2221 gccgccagtc gcgggttaga gacctgaaaa caatgacggg tagaggcttc attaccaaac
     2281 gacaggcccg cgtaatgatg cgtcagccct accagcccgt cgaaattgac ttcccaggcg
     2341 ttcatcgcac cacctcatcg gaaaaatcca gcccagggtt aagcgtggcg ggcaatgtta
     2401 acgagtccga ctccaggctc gccatcggcc aggcacaata atctgcggca taccaggcgc
     2461 tggggcgatg gttaccggaa gcgccaatgc cgccgaatgg cgcggtactg gcagcgccgg
     2521 taagcggttt gttccagtta acaatccccg cccgcgcctc cagcaacagt tgatcgaatt
     2581 tttcccgctc gggggaaact agaccgcaag agaggccaaa gcgagtgtta ttcgccattc
     2641 gaatcgcttc atcgaaagta tcataacgcc agacgcgcaa taacggtccg aacacttctt
     2701 catccggcaa gccagtaacg cctgtcattt caatgatccc cggcgtcagc aacgatgtcc
     2761 ctgcttgtaa taagcgcggc gcaagcaggg tccgtccgcc catcgcctcc agttcctgcc
     2821 aggcagtaac cacctgctgt gcggcctgct cagaaatcag cccaccaata aatggctgcg
     2881 gttcgtcatc ccaagtgccc ggcgttaacc gctggctgac ggcaaccagg cgagcaagaa
     2941 atgcatcgcc ctgcgttcca cttttcagaa ataaacggcg ggcgcaggtg cagcgttgcc
     3001 cggcagtgac aaacgccgac tgaatggtca gatggacagc ggcatcgata tccgccacct
     3061 catcgataat tagcgggtta ttaccgccca tctcaagggc gagaattttc tccggctgac
     3121 cggaaagctg gcgatgcagc tggtagccgg tattggcgct gccggtaaac agcaagccgt
     3181 cgagatcctc cagcgcactc agcgcctgac ccgttgcacg cccgccctgc accaggttca
     3241 gcacgcccgg cggcaagcca gcctgctgcc ataaacgcat taccgcgtcg ccactccacg
     3301 gtgtcagttc gctgggttta aagataatgg tgttacctgc cagcaatgcc ggaacgatat
     3361 gtccgttcgg caaatgacca gggaaattat acggcccaaa caccgccagc acgccgtgcg
     3421 ggcgatgtcg caggctcgcc gcgccgtccg gcatttcgct acgctgctca ccggtacgaa
     3481 cgtgatacgc cttaattgat atcgcgattt tattgatcat cgccgtcact tcggttgccg
     3541 cttcccagcg cggcttgccg gtttctctgg caataatcgc ggttaattcg gctttattgc
     3601 tttccagcaa tccggcaaag cgttcaactc ttacctgacg atcgccaaat gagagccgag
     3661 cccagcgcgg aaacgccgca cgggcagccc gacaagcctg cccgacctga gcggcatcgg
     3721 catcattgcc ctgccataac acctcgcccg ataccggatt acgcttcaca cgcaatgcgc
     3781 cctggcccgt tacccagtca ccgttgatcc ataaagtcat gctgttttct cctctgcgca
     3841 caggcgcacc agacgaacgc gatcccctgc gtggcatttg agggcatcca gttgtgcggc
     3901 ggttaaaatc aaacgctcgg tcgctggatc ggtacgcacc agcaccacgc ggaaatggtg
     3961 ataattttca ttggcgacca ggcaggccgg aaaatcgccc tgcgcaggct gcccttcggc
     4021 cacttccacc agccgacttt tacggatggc gcgcacgcgg tcgatatcac attcaagtgt
     4081 cggcccgccg tcaaagatgt cgatatagtt acggtagcga aaaccttctt tctccagcac
     4141 cgcacgggca ggcgcggttt gcggatgtac ctgaccgatg acgtcctggg cttcctggga
     4201 taaaaagtgg gtatagatcg gatgtttcgg catcagttct gcaataaatg ccttttgccc
     4261 ggtgccgcag agaaaatcag cgcggctaaa atccatcgaa aagaagcgtt taccgaggct
     4321 ttgccagaac ggtgaatagc cgtgttcgtc aatcaccccg cgcatttcgg cgaccacttt
     4381 gtcattaaac ttgtcgcgaa aagccgccat aaacataaag cgcgatttcg acagcaaata
     4441 gccgttgccc tctttgcgcc actccgggtc gagaaacagc gtgcacagct cgctgctgcc
     4501 ggtgtgatcg ttactgagaa acagcgtcgg caatgcgtta tagacattca gctctttcga
     4561 agcgtgaacc agcgtgccga cacgatagtt gtaccaggga tcgttcagcc caaccgccac
     4621 ctcaatggcg caaatccccg ccacagtgcc tgtctcacta tcttccagta caaacacata
     4681 accctgctca cttttgggca gttcgccttg ccaggttttg attgcccttt caatacgcac
     4741 cgaaagcgtg gcttcattgg cgggaagcga cgtcaggccg ccgcccgttt tgctggcaag
     4801 ctgcatcagc gccgagacat ctgagcgctc aacgggacga atgaccatca tgatgaacct
     4861 cggctaacaa agtgttcgca agcagctgca aagcgatcca gtccggtcgt cacctcttct
     4921 tcgctgacat tgagcgcagg cgcaaagcgc accacgttgc cacccgcaat cagcaccatc
     4981 acgcctgctt tcaccgcttc ctgagagatc tgtttcgctt gcccggcgta atcggcattc
     5041 agtacacagc caatcaaaag acctaagccg cgaacttcac tgaacaaact gtagtggtga
     5101 ttaatggaat ttagacgctc aacaaaccag tcgtgacgct gtttaacccc gttaagcatc
     5161 tctggcgtgt tgatgagatc cagcactttg cctgccaccg ccgaggccag cggattgccg
     5221 ccataggtgg tgccgtgagt gccaacggtc atcacgctgg cgcactcttc ggttgccaac
     5281 aacgcaccaa ccgggaaacc gccgcccagc gctttggcgg tagttaacag atcaggcgtc
     5341 acgccgtagt gcatacaggc atacagttcc ccggtgcgcc cgacaccggt ttgtacttca
     5401 tcaaagatca gcaacgcatt gtggcggtca cacaattcac gcagaccttg taaaaatgcg
     5461 ttgctggctg gcaccacacc gccttccccc tggatgggtt cgacaatcac cgcacaggtg
     5521 gcgtcgtcaa tcagcgcact ggcagagtta atatcgttat atgcagcatg acgaatatcc
     5581 ggcggcagtg gcgcaaaatc ctgtgaatag gctggctgcc cacccgcact gacagtaaac
     5641 agcgtgcgac catgaaacgc atttttgaac gccacgatgc cgctcttatg gctgccgtag
     5701 cggtcgtgag cgaatttacg cgccagtttt agtgccgctt cgttggcttc cgcaccggag
     5761 ttacaaaaga agacgcgatc ggcaaacgtg gcgtcgatca attttttcgc cagtcgcagt
     5821 accggctcgt tggtgtaacc gttgccggta tgccagaact tactcgcctg ttcgttcagc
     5881 gcctcacgca gttccggatg cgcatggccc agcgcgttca ccgcaatgcc acccgcgaag
     5941 tcgatatact ctttcccctg ctgatcccac aagcgtgaac cttcgccacg taccggtata
     6001 aacggtgccg gagcgtaaac aggtatcatc cattcatcaa agttttcacg cgtaattggc
     6061 tgagacatag cgacctctac agtaaataat tcgttattta tatgttaata ataagtaatg
     6121 tttgcgctgt aaatgtagat tgcagggttc gtgccagcca gtgataaaag tgcataaacg
     6181 gcggaggcta actggaaatc aagcagttat aaccacatca tatgcattta aagtgcatat
     6241 aaagtgaata cgtttgcgat gtgggtgaat aaaaagaata aaaaacgcaa tgttatgcag
     6301 aagtaaaata taattctgga attgtgatca ttgacgaaat ttactggaaa ttactgcgcc
     6361 attctgacgc aacgcgcacc aaaagcgggc attttttgcg ccatcgttga catcattaac
     6421 aaccatcgat caaatcactt aacaacaggc ggtaagcaaa gcgaaattct gctaccatcc
     6481 acgcactctt tatctgaata aatggcagcg actatgaaat ttgtctcttt taatatcaac
     6541 ggcctgcgcg ccagacctca ccagcttgaa gccatcgtcg aaaagcacca accggatgtg
     6601 attggcctgc aggagacaaa agttcatgac gatatgtttc cgctcgaaga ggtggcgaag
     6661 ctcggctaca acgtgtttta tcacgggcag aaaggccatt atggcgtggc gctgctgacc
     6721 aaagagacgc cgattgccgt gcgtcgcggc tttcctggtg acgacgaaga agcgcaacgg
     6781 cggattatta tggcggaaat cccctcgccg ctgggcaatg tcaccgtgat caacggttac
     6841 ttcccgcagg gtgaaagccg cgaccatccg ataaaattcc cggcaaaggc gcagttttat
     6901 cagaatctgc aaaactacct ggaaaccgaa ctcaagcgtg aaaatccggt gctgattatg
     6961 ggcgatatga atatcagtcc tggcgacctg gatattggca ttggcgaaga gaaccgtaag
     7021 cgctggctgc gtaccggaaa atgttctttc cttccggagg aacgcgaatg gatggagagg
     7081 ctgatgagct gggggttggt cgataccttc cgccatgcga atccgcaaac agcagatcgt
     7141 ttctcatggt ttgattaccg ctcaaaaggt tttgacgata atcgcggtct gcgcatcgac
     7201 ctgctgctcg ccagccaacc gctggcagaa tgttgcgtag aaaccggcat cgactatgaa
     7261 attcgcagca tggaaaaacc gtccgatcac gcccccgtct gggcgacctt ccgccgctaa
     7321 tttagctgat ctcctggctc aaactgggtc aggagaatta accttgagaa aaatcaacaa
     7381 actgtcagta atgatttgtt gcctgccgtc ctttgttata ccgtcactgc gtttttagtt
     7441 gtctgaccac ttctctatta tcaagtttga tataggaaac tccacgatga acgctgagcg
     7501 taaatttctt ttagcctgtc ttatttttgc gctggtcatt tacgctatcc acgctttcgg
     7561 tttattcgat ctgctcaccg atttacccca cttacagaca ctcattcgcc agagcggatt
     7621 gttcggctat agcctctata ttctgttatt catcattgcc accctctttc tgttaccagg
     7681 aagcatactg gtgatcgcgg gaggaatagt ttttggcccg ctcttaggga cactactatc
     7741 attaattgcc gccacgctgg cctcctcgtg ctcattcctg ctggcgcgct ggctgggacg
     7801 tgatttactg ctgaaatacg ttggtcatag ccataccttt caggccattg aaaaaggcat
     7861 tgcgcgtaac ggtattgatt ttcttattct gacccgctta ataccgttgt ttccttacaa
     7921 tattcaaaat tacgcttacg gattaaccac aatcgccttc tggccttata cccttatttc
     7981 ggcactcacg accctacccg gtattgttat ttataccgtg atggcaagcg atctcgccaa
     8041 tgaaggcatt acgctgcgct ttattttaca actctgtctg gcgggcctgg cgctgtttat
     8101 tctcgtccag ctcgcaaaac tctacgcccg acacaaacat gtggatctgt ctgcttctcg
     8161 ccgcagccca ctttctcacc caaaaaatga aggatagaac gatgttgcaa cattattcag
     8221 tgtcatggaa aaaaggactg gctgcactct gcttactggc tgttgctggg cttagcggct
     8281 gcgatcaaaa agagaatgcg gctgcaaaag tggaatacga cggactttcg aacagccaac
     8341 cgctgcgtgt cgatgccaat aaccatacgg taaccatgct ggtgcaaatt aatggtcgtt
     8401 tcctcaccga cgacactcgt cacggtattg tgtttaaaga tggctccaac ggacataaat
     8461 cgctgtttat gggttatgcg accccgaaag cattttatga agccctgaaa gaggcaggtg
     8521 gtacgccggg cgaaaacatg acgatggata ataaagaaac gactcatgtc acaggcagca
     8581 aactggatat ttcggtcaac tggcaagggg cggcaaaagc gtattccttc gatgaagtga
     8641 ttgttgacag taatggcaag aaactggaca tgcgctttgg cggtaattta acggcagcag
     8701 aagagaagaa aacgggttgc ctggtgtgtc tggatagctg cccggtcggc atcgtcagca
     8761 atgcaacata cacttatggt gcggtggaaa aacgcggcga agtgaaattc aaaggcaatg
     8821 cctcagttct cccggcggat aacacgctgg caacggttac ctttaaaatc accgaataaa
     8881 gcaggataac ggatgatgaa gatgcaatcg cgtaaaatct ggtactaccg tataaccctc
     8941 atcatcctgt tgttcgctgt gctgctggca tgggcgctgc ttcccggcgt ccatgagttt
     9001 atcaatcgca gcgttgcggc gtttgccgcc gtggaccaac agggtataga acgctttatt
     9061 cagtcttacg gcgcactagc ggcagttgtc tcgttcttgt tgatgatttt acaggccatt
     9121 gctgcaccgc tacctgcgtt tttgatcacc tttgccaatg cgtcgctgtt tggtgcgttc
     9181 tggggcggct tactgtcgtg gaccagttcg atggccggcg cggcactgtg cttttttatc
     9241 gccagagtga tgggccgcga agtggtggaa aaattaaccg gcaaaaccgt acttgacagt
     9301 atggacggct ttttcactcg ttacggcaaa cacaccatcc tggtatgtcg gttattgcct
     9361 tttgtccctt tcgatccaat cagctatgct gccggtttga cttcaatacg ttttcgctcg
     9421 ttttttatcg ccaccgggct tggtcaatta ccggcgacta ttgtttattc ctgggcgggc
     9481 agcatgttaa caggcggtac tttctggttt gtcaccggac tgtttattct gtttgccctg
     9541 accgtggtga tttttatggc gaagaaaata tggcttgagc gccagaagag gaatgcctga
     9601 tgggtttacc gccgcttagc aaaattcctt tcattttacg tccgcaggcg tggctgcatc
     9661 gtcgccatta cggcgaggtg ctaagcccca ttcgctggtg ggggcggatc ccgtttatct
     9721 tttatctggt gtcgatgttt gtcggctggc tggagcgcaa acgctcaccg ctcgatccgg
     9781 tagtacgatc gcttgtcagc gcgcgcattg cgcaaatgtg cctgtgtgag ttttgcgtgg
     9841 atatcaccag tatgaaagtc gccgagcgta ccggcagcac cgataaactg ctggcagtgg
     9901 ctgactggcg gcaaagcccg ctctttagcg atgaagaacg gctggcgctg gagtatgccg
     9961 aagccgcaag cgtaacgccg ccaacggtcg atgatgccct gcgcgcccga ctggctacgc
    10021 attttgacgc tcaggcgctc accgaactga cggcattgat aggcctgcaa aatctgtcag
    10081 cccgttttaa ttctgccatg gacattcccg ctcaggggct gtgccgtatt cctgaaaaac
    10141 gttcttaagg agagaagatg cgccattgtg ggtggttgct gggattgtta tcgctgtttt
    10201 ctttggcaac acatgccagt gactggcagg aaataaaaaa tgaggccaaa ggacaaaccg
    10261 tctggtttaa cgcctggggc ggcgataccg caattaaccg ctatctcgac tgggttagcg
    10321 gcgagatgaa aacccattac gctataaacc tgaagattgt tcgtctcgcg gatgccgcag
    10381 acgcggtgaa gcgcattcag accgaagctg ctgccggacg caaaacgggc ggttcggtgg
    10441 atctgctctg ggtgaacggc gaaaacttcc gcaccttaaa agaggccaat ttattacaaa
    10501 cgggctgggc ggagactctg cccaactggc gctatgtcga cacacagctt ccggtgcggg
    10561 aagatttttc agtgcctaca gaaggggctg aatcgccctg gggcggcgca caactgacat
    10621 ttatcgcccg ccgcgatgtt acgccacagc caccacaaac gccgcaaacc ttactggagt
    10681 ttgctaaagc caatcccggc acggttacct atccgcgccc accggacttt accggcacgg
    10741 cgtttcttga acagttgctg attatgctaa cgccagatcc cacagcatta aaagaagcgc
    10801 cggacgatgc gactttcgcc cgcgtcactg cgcccttgtg gcaatatctt gatgcactgc
    10861 atccgtattt atggcgcgaa ggaaaggatt tcccgccttc gcccgcgcgg atggatgctc
    10921 tgctgaaagc taacacattg cgcctgtcgc tgacctttaa ccctgcacat gcgcagcaaa
    10981 aaatcgccag cggcgatttg cctgcaagca gttacagttt tggttttcgc gaggggatga
    11041 ttggcaacgt ccatttcgtc accattcctg ccaacgcgaa tgccagtgct gcggcgaagg
    11101 tagttgccaa tttcctgctc tcacccgatg cgcaactgcg taaagcagat cccgctgtct
    11161 ggggcgatcc ttctgttctc gatccgcaaa aactgcctga cgggcagcgc gaaacattgc
    11221 aatcgagaat gccgcaggat ctgccgccgg tactggctga accgcacgca ggttgggtaa
    11281 atgcgctgga acaagaatgg ctacaccgtt acggtacgca ttaatctttt tgctgtgggc
    11341 gatgatagcg gtgatttatg caccgctgat cccggcagct ctgacgctga tatcgcctgc
    11401 tttgtcgttg acgcactggc aggcgttatt tgccgatcca cagttaccgc aggcattact
    11461 ggcaacgctg gtgtcgacaa ccattgcggc ggtcggggcg ttgttgattg ccctgctggt
    11521 gattgtggcg ctgtggccag ggccgaaatg gcagcgtatg tgcgcccgtc tgccgtggct
    11581 gctcgccatt ccccatgtgg cttttgccac cagcgccctt ctgatctttg ctgacggagg
    11641 gctgctttat gactatttcc cgtatttcac tccgccaatg gacaaattgg gtattgggct
    11701 gggcctcacc ctggcggtaa aagaaagcgc atttctgctg tggatcttag cggcagtatt
    11761 gagcgaaaaa cggctgttgc agcaactcat tgtgctggat tcgctgggct acagccgctg
    11821 gcaatgcctg aactggttgc tgttgccctc cgtcgcgtct gcgctggcaa tggcgatgct
    11881 ggcgattgtc gcctggtcgc tgtcggtcgt ggatgtggcg attattcttg ggccaggtaa
    11941 tcccccgacg ctggcggtaa ttagctggca gtggttaact cagggcgacg ccgatcaaca
    12001 aacgaaaggt gcactcgcca gcctgctgct gatggtgtta ctcgccgcct acgttttgct
    12061 gggctatctg ctatggcgtg gctggcgacg tactattccc cgcgtagatg gcgttcgtaa
    12121 gcctgccacg cctttattgc caggcaatac gctggcgagt tttttaccct taaccggtgt
    12181 gctgtgtgtg gttctgctgg cgatcctcgc ggatcagtcg acgatcaata gtgaagcgct
    12241 catcaacagc ctgacaatgg ggctggtggc ggcattcatc gctttgctcc tgttactgct
    12301 gtggcaggaa tgggggccac agcgtcgcca gttgtggcta tggttgccca ttttattacc
    12361 cgctctgcct ctggtggcgg gccagtacac gctggcgcta tggctgaacc tggatggaag
    12421 ctggacggcg gtggtctggg ggcatctgct gtgggtaatg ccgtggatgc tgtttatcct
    12481 gcaacccgcc tggcagcgca ttgattcacg gttaattttg attgcgcaaa cactgggctg
    12541 gtcgcgggcc aaaatcttct tttacgtaaa atgtccactt atgttgcgcc ctgcgctgat
    12601 tgccttcgcg gtgggatttt cagtcagtat tgcgcagtat atgccgacgc tgtggctggg
    12661 cgcggggcgt tttccgacgc tcaccactga agcggtggca ttaagcagcg gcggcagcaa
    12721 cggtattctc gccgcccagg ctttatggca actgctgtta ccgcttatta tttttgccct
    12781 gaccgcgtta gtcgcaaaat gggtaggtta tgtcagacaa ggactccgct aatgctctgc
    12841 gtgaaaaatg tttcgctacg tttaccagaa agccgcttgc tgacaaacgt taactttaca
    12901 gtagataaag gtgacattgt cacgttaatg ggaccatccg gctgcgggaa atccactctg
    12961 ttttcgtgga tgattggtgc gctggccgga cagttttctt gtacagggga gctatggctt
    13021 aatgagcaac ggattgacat gctacccacc gcacagcgtc agattggcat tcttttccag
    13081 gatgcactgt tatttgacca gttcagcgtc gggcaaaatt tactgctggc gctaccggcg
    13141 acacttaagg ggaatgcccg acgtaatgcc gtgaatgatg cacttgagcg ttcaggcctt
    13201 gacggagcgt tccatcagga tcctgccact ttgtctggcg gtcagcgagc gcgcgttgct
    13261 ctgctgcgcg cccttctcgc tcagccaaaa gcgttgctac tggatgagcc cttcagccgt
    13321 cttgatgtgg ccctgcgcga taattttcgc cattgggtgt tcagcgaaat tcgcgccatg
    13381 gcgatccctg ttgtgcaggt gacgcacgat cttcaggatg ttcctcctga tagccccgtt
    13441 ctggatatgg cgcagtggtc agaaaattac aacaaactgc gataacgcaa agtttttccc
    13501 gatgcgtcag ttcagaatgg cgctctcaaa actacaatgt cgggattttc gatgaaacgt
    13561 gtttctcaaa tgaccgcgct ggcaatggct ttagggctgg cttgcgcttc ttcgtgggcc
    13621 gctgaactgg cgaagcctct tacacttgac cagcttcaac aacaaaatgg caaagcgatt
    13681 gatactcgcc ccagcgcgtt ttataacggc tggccacaaa ccttaaatgg cccttccggt
    13741 catgaacctg ccgccttaaa cctctctgct agctggctcg acaaaatgag caccgaacag
    13801 ctcaacgagt ggatcaagca acataacctg aaagccgatg ccccggtggc gctgtacggt
    13861 aatgacaaag atgtcgacgc cgtcaaaacg cgactgcaaa aagcaggttt tacgcatatc
    13921 tccatcctga gtgacgcgct aagcgaacct tcccgtctgc aaaaactgcc gcactttgaa
    13981 cagctggtat atccgcagtg gctgcatgac ctgcaacaag gtaaagaggt tacggcgaaa
    14041 cctgccggtg actggaaagt cattgaagcg gcctggggcg ctccaaaatt ttaccttatc
    14101 agccatattc ccggcgctga ctacatcgac accaacgaag tggaaagcga accgctgtgg
    14161 aacaaagttt ctgatgaaca actgaaagcg atgctggcaa aacacggcat tcgccatgac
    14221 accacggtca ttctgtacgg gcgtgacgta tacgctgcag cgcgtgtggc gcaaattatg
    14281 ctttatgccg gcgtgaaaga tgtgcgcctg ctggacggag gctggcaaac ctggtccgac
    14341 gcgggtctgc ccgttgagcg cggaacgcca ccgaaagtga aagcggaacc ggatttcggc
    14401 gtgaagatcc cggcacaacc gcaactgatg cttgatatgg aacaagcgcg tggactgctg
    14461 catcgccagg atgcatcgct ggtgagcatt cgttcgtggc cagaatttat cggtacgacc
    14521 agcggttaca gctatattaa accaaaaggt gaaatagccg gagcacgttg gggacacgca
    14581 ggtagcgact cgacgcatat ggaagatttc cataacccgg atggcaccat gcgcagcgcc
    14641 gatgatataa ccgctatgtg gaaagcatgg aatatcaaac cagatcagca agtttcattc
    14701 tactgcggca ctggctggcg cgcgtcagaa acctttatgt acgcacgcgc aatgggctgg
    14761 aataacgtct ccgtttacga cggtggctgg tacgaatgga gcagcgatcc aaaaaatccg
    14821 gtagcaaccg gtgaacgcgg cccggacagc agtaaataac attgcaatta ctgacgctgg
    14881 agcgacttca gcgtcagata cccactccac acccgcgtaa aggtcgtcat ccagcacaac
    14941 gcaccaaata cccacgcaaa ccacggaaaa caggccggaa agaggcaact cagaacaaac
    15001 agcaaaatcg tctcgctgcc ttcggttaac ccgcccagat aataaaacga tttatgcgca
    15061 taaccaggat tatcaatctg atgtttcgcc gccagcgcag caaaagcgag aaaactgctg
    15121 cccgtgccga taaacgcaaa cagcagccag ccgcctacca gggcgttttg ctccggtgcg
    15181 gcgaggataa acccaaaagg caccagcgcg taaaaaagaa aatcgagaga aatatcgaga
    15241 aagccgcccg cgtcggtaag ccctctgcgc cgcgccagcg caccgtccag accatcaagc
    15301 agcctgttca acaaaatggc gactagtgcc gccagatacc agcccagcgc cagaaaaggc
    15361 agtgccagca cgccgatggc aaatcccact aatgttaaac catctggcgt aatgcccggt
    15421 ttatcaagaa cccgcacgca ctgatgcaac aacggtttaa tccggggatg aagatggcgg
    15481 tctagcacag gcactcctta aatataaagc ctttctgatt gagcaacagt gcggatatta
    15541 tggcattttt cgcttatctg cccgtgtgta atttatgaaa atgattgaag ttgttgccgc
    15601 catcatcgaa cgtgatggca aaattttact cgcgcaacgc cctgcccata gcgatcaggc
    15661 gggattatgg gagtttgccg gtggtaaagt cgagccggat gaaagccagc ggcaggcgct
    15721 ggtgcgtgag ttaaacgaag aactgggcat cgaagcaact gttggtgact atgttgccag
    15781 ccatcagcga gaagtttcgg ggcggattat ccatcttcat gcctggcacg tacccgactt
    15841 ccacgggacg ttacaggcac atgaacatca ggcgctggtc tggtgctcac ctgaagaggc
    15901 gctgcgatat ccgctggccc ctgctgacat tccattatta gaggcgttta tggcttcacg
    15961 cgccgccaga ccagcggatt agtgcttagg gttttgtcat cccgttggca ttgcagcagt
    16021 attccttcgg ctttaattac tgccccttca gaataatttt gatcctgata aacgcagcac
    16081 tgagtacagg gctgcgctga ctgcccgcct gaactaaaca cttctggcgg tacgtttacc
    16141 tccacgtccg gatgataatg cgggttagcc agtgtaatta atggaaatgc taatactacg
    16201 gcgatcaatg ctcgactcac agggaactcc ttaacgttat tgtctcttct acagataacg
    16261 gtagccgggc ggcaaaactt tagcgtctga gttatcgcat ttggttatga gattactctc
    16321 gttattaatt tgctttcctg ggtcattttt ttcttgctta ccgtcacatt cttgatggta
    16381 tagtcgaaaa ctgcaaaagc acatgacata aacaacataa gcataatcgt attaatatat
    16441 aagggtttta tatctatgga tcagacatat tctctggagt cattcctcaa ccatgtccaa
    16501 aagcgcgacc cgaatcaaac cgagttcgcg caagccgttc gtgaagtaat gaccacactc
    16561 tggccttttc ttgaacaaaa tcccaaatat cgtcagatgt cattactgga gcgtctggtt
    16621 gaaccggagc gcgtgatcca gtttcgcgtg gtatgggttg acgatcgcaa ccaggtgcag
    16681 gtcaaccgtg catggcgtgt gcagttcagc tctgccatcg gcccgtacaa aggcggtatg
    16741 cgcttccatc cgtcagtaaa cctttccatt ctcaaattcc tcggcttcga gcaaaccttt
    16801 aaaaatgccc tgacaactct gccgatgggt ggtggtaaag gcggcagcga ctttgatccg
    16861 aaagggaaaa gcgaaggcga agttatgcgt ttctgccagg cgctgatgac tgaactgtat
    16921 cgccacctgg gcgcggatac tgacgttccg gcaggtgata taggcgttgg tggtcgtgaa
    16981 gtcggcttta tggcggggat gatgaaaaag ctctccaaca ataccgcctg cgtcttcacc
    17041 ggtaagggcc tttcatttgg cggcagtctt attcgcccgg aagctaccgg ctacggtctg
    17101 gtttatttca cagaagcaat gctaaaacgc cacggtatgg gttttgaagg aatgcgcgtt
    17161 tccgtttctg gctccggcaa cgtcgcccag tacgctatcg aaaaagcgat ggaatttggt
    17221 gctcgagtga tcactgcgtc agactccagc ggcactgtag ttgatgaaag cggattcacg
    17281 aaagaaaaac tggcgcgtct tatcgaaatc aaagccagcc gcgatggtcg agtggcagat
    17341 tacgccaaag aatttggtct ggtctatctc gaaggccaac aaccgtggtc tgtaccggtt
    17401 gatatcgccc tgccttgcgc aacccagaac gaactggatg ttgacgccgc gcatcagctt
    17461 atcgccaatg gcgttaaagc cgtcgccgaa ggggcaaata tgccgaccac catcgaagcg
    17521 actgaactgt tccagcaggc aggcgtactg tttgcaccgg gtaaagcggc taatgctggc
    17581 ggtgtagcaa catcgggcct ggaaatggcg caaaacgctg cgcgcctggg ctggaaagcc
    17641 gagaaagttg acgcacgttt gcatcacatc atgctggata tccaccatgc ctgtgttgag
    17701 catggtggtg aaggtgagca aaccaactac gtgcagggcg cgaacattgc cggttttgtg
    17761 aaggttgcgg atgcgatgtt aagccagggc gtgatttaag acaattgtgc cggatacagc
    17821 gcaaacgcct tatccggcca taaaaatctt cagtatcaga tggttgaaag aacctttatg
    17881 ttatcactcc attgcccgcc tcttctgatg gcgggctttt tttcggcgct ctcttgcgtg
    17941 gcgaatcctt tttcttccct tctctaccgc cgggagccat tattccccga aactgcctta
    18001 ccgacgtgcg ctttgcctga tcaatcaact ggtacaatgt ccccaccagc ggctgcataa
    18061 agtcctgata gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg
    18121 tcatgtccgg tcgcgtcgcc atttctggca gcgaatggaa tagcgctttt ccggcgtcgg
    18181 tggagtggat atagcgccct tttttggtca ggaaaccccg cttgaacaac aattcaataa
    18241 tcccggcacg cgttgcttct gtccccaaac catcggtcgc acgaaggatc tttttcagat
    18301 ctttgtcctg cacaaagcgc gcgatcccgg tcatcgccga aagcagtgtt gcatcggtaa
    18361 aatggcgcgg cggctgggtt tgccgctcta ccacttcgcc tttttcgcac aacaattcat
    18421 cgcctttcgc caccacaggc agcggcgtgc cgtcgttttc ttcatcgcgc tctttgctgc
    18481 ctaacagcgt gcgccagcct gcttcagcaa gaaaacgggc tttagcgaca aatttgcctt
    18541 tggcaatgtc cagttcgata acacacttgc ggaacaccgc atccgggcag aactgcatca
    18601 agtactgacg ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag
    18661 aactccgtgc ggtcggaatg atggcgtggt gcgcatcgac ctttttgtca tcccaacagc
    18721 ggttgcgtat atctggatct accactggct gcggcaacag atccggtgca tgaacgctga
    18781 tagcattcat caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cggcaatcgg
    18841 aacgcggata agtgattagc ttgtgcgttt cgtacagctt ctggcagata tcaagcacgt
    18901 tctgcgcgct cagaccaaaa cgtttagccg cttcaatctg caacgccgaa agcgaaaaag
    18961 gcagcggcgc ggattctgat tcccgtttat cgttatagct ggtgacaata gctggttgac
    19021 cactaatgcg gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat
    19081 cctggtacgg ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag
    19141 gtgtcacgat atgtgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt
    19201 cgtcgcggcg caccaccagt ccgagtacgg gcgtctgcac gcgtcccacg gaaagcacgc
    19261 cctgataacc ggcattgcga ccgagaatgg tatacgcacg ggtcatatta atgccgtaca
    19321 gccagtcagc ccgcgcccgc gccagtgcag aaacgcacag cggtacaaac tcgctattgg
    19381 agcgaagacg atcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaaacaac
    19441 gctgtacctg ctggcgcttt tccggtgcca gttgcaggta gtccagcact tcatccacca
    19501 gcagttgccc ttcgcgatcc gggtctccgg cgtgaacgat ttcgctggct tcatgcagga
    19561 agcgtttgat gacattaagt tgtttagtca cggagggtcg gggctgtaat tgccactttt
    19621 ccgggacaat cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg
    19681 gctgcgcctg ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact
    19741 cgataaagcc atcgcctttc cggtgcggtt taggcaggac atcagcaatg gcgcgcgcca
    19801 gactcggttt ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga
    19861 ccgccgcgcg ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct
    19921 gtagctttga cctcattttc tgcttccggc atgaccgcca gcagcaaacc gccagaagtt
    19981 tgcggatcgc acagcagatc gcgcacttca cgcggcattt cacccatcag atgaccgtag
    20041 ctggcaaagt tacgttcggt gccgccaggc actgcgccca acttaatgta ctcttcaacc
    20101 cccggcaatt tcgggatcgc gtcatagtcg acgcgcgcct gcacacctgc gccctgacac
    20161 atttcgctta aatgccccag caaaccaaaa cccgtaacgt cggtcatcgc ttttacgcct
    20221 tcgatgttgg caaaggacgc gcctgcgatg ttcatccggc acatcacttc cgtcgccagt
    20281 ccctgatgtt ctggtttcag cagtgatttt ttctcagccg tggtaagaac gccaatcccc
    20341 agcggtttcg tcaggaacag tttgcatccg gcttgtgcgg tactgttttt cttcacccgc
    20401 tcggtcggta cgatccccgt taccgccaga ccaaaaatcg gctccggcgc atcgatggag
    20461 tgaccgccag ccagcgcaat acccgcctga cggcatgcat agcgtccacc ttcggtcact
    20521 tcgcgggcaa tttccgggga aagtttgtta atcggccagc cgaggatcgc aatcgccata
    20581 atcggtttgc cgcccatagc aaagatatcg ctgatggcgt tagtcgccgc aatgcggcca
    20641 aaatcgaaag gattatcaac gatcggcata aagaagtcgg tggtactgat aacgctggtg
    20701 ccattgccca gatcgtatac cgccgcatcg tcgcgggttt cattacccac aagcaaattc
    20761 ggatcaacaa acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt
    20821 ttacagccgc aaccagcgcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg
    20881 gacatctcct gtcaatgcaa tccgggtatg gtaaccctca ttccatgaag tgataagtga
    20941 gagtgtctga attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga
    21001 aataagtcac aaacggcgtc gggtccggga cgtttatcga cgtagatgct ttcaactgcg
    21061 gcgtaccgag gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatgcttcac
    21121 gcactaccgg actttcagtt aatgcgccac tgcgccagat gccgccaaac ccctgggcaa
    21181 ctgctgccat ttgcatcgcc atgaccgcgc atcccgcaga catttcctgt tcccagcgcg
    21241 ggactttatg attctcttcg cattttgcca ccaccgttat gatgagcggt gcgcggaacg
    21301 gcgcattacg cgctttgtcg attgctttgt tatcactacc gacagcaatc gccccctgct
    21361 ccagtacagc gctgaaacgc tcgcgccctt caccttcaat cacaaagaaa tgccacggtt
    21421 gcatggactt atggtccggc gcacgcatac ccgcacgcag gatgttttgc agttgttcac
    21481 ccgttggcgc gggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg
    21541 catccatttg attaactcct gtcatgatat ttattcacaa aattaacacg agagtggatt
    21601 ttgttacagc ccagtccgca attcctgctg acaagtgccg gttgggtcat tacgataacc
    21661 acatctattg cgcctgaaac aggtgtgacc ttaagttggg agaatacatg cgaacccttt
    21721 ggcgatttat tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa
    21781 tggtacttaa cctgttcttt atttttctcg tgctggttgg tgtggggatc tggatgcagg
    21841 tcagtggtgg tgattcgaaa gaaacggcca gtcgtggcgc actactgctg gacatttctg
    21901 gtgtgatcgt cgataaaccc gacagttctc agcggtttag taaattaagc cgccagctgc
    21961 ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aacaccattc
    22021 gccaggcgaa ggacgaccgc aatatcaccg gtattgtgat ggatctgaaa aacttcgcag
    22081 gcggcgacca gccgtctatg cagtacatcg gcaaagctct gaaagagttt cgtgatagcg
    22141 ggaaaccggt ttatgccgtt ggcgagaact acagccaagg gcaatattat ctcgccagtt
    22201 tcgccaataa aatttggctg tctccgcaag gcgtggttga tctgcacggc tttgccacca
    22261 acggtctgta ctacaaatcg ttactggata agctgaaagt ttccacccat gtgttccgcg
    22321 tgggtacgta taaatctgcc gttgaaccgt ttattcgtga tgatatgtcg ccggcagccc
    22381 gcgaagctga cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg
    22441 ctaaccgcca gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgagggtt
    22501 taaccaaaac cggtggcgat accgcgaaat atgcgctgga aaacaagctg gtcgatgcac
    22561 tggcatcgag tgccgaaatc gaaaaaactc tgaccaaaga gttcggctgg agtaaaactg
    22621 ataaaaatta tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg
    22681 gtgacagtat cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag gaaactcagg
    22741 ggaatgttgg cggtgatact actgcggcac aaatccgcga cgctcgcctt gacccgaaag
    22801 tgaaagcgat tgttctgcgt gttaatagtc ccggcggcag cgttaccgcg tctgaagtga
    22861 ttcgcgctga actggcagca gcccgggcag cgggtaagcc tgtggttgta tcgatgggcg
    22921 gcatggcggc atctggtggt tactggattt ccacaccagc taattacatt gtggctaacc
    22981 ccagcaccct gaccggttct atcggtatct tcggcgtgat caccaccgta gaaaatagtc
    23041 tggattcgat tggtgttcat actgatggtg tctcaacttc accgctggca gatgtttcta
    23101 tcaccagggc actgccgccg gaagcgcagc agatgatgca gttaagcatt gagaatggct
    23161 ataaacgctt tatcacgctg gttgctgatg cgcgtcattc aacgccggag cagattgata
    23221 aaatcgccca gggtcacgtc tggaccggtc aggatgcaaa agctaacggg ctggtcgata
    23281 gtctcgggga tttcgatgat gcggtcgcca aagcagcaga gctggcaaaa gtgaaacagt
    23341 ggcatctgga atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaacatgt
    23401 ctggttctat ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgctgg
    23461 cctcggtagc ctctaccgtt aaaagtgaaa gcgacaagct ggccgcgttt aacgacccac
    23521 aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg tactaagtgg
    23581 ccgacagatc gtcggccaca ttacttttta cgtcgacgaa tcctcttccc gctgtctcgc
    23641 cacatatttc cttatcctca tagtatcagg tgcgctcccc ctgcctcacg tatatacttt
    23701 tgctctttcg atatcattca tatcaatatc atgcaaaaga agtcaattta cgtcgcctac
    23761 acgggcggga ccatcgggat gcagcgttcc gagcagggtt atattccggt gtcaggtcat
    23821 ctacagcgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc agatttcacc
    23881 attcatgaat atacgccatt gatggattct tctgatatga cgccagaaga ctggcagcat
    23941 attgctgaag atattaaagc gcactatgac gattatgatg gttttgtcat tctgcacggt
    24001 acagacacga tggcgtatac cgcctctgca ctgtcgttca tgctcgagaa tctcggtaaa
    24061 ccggtcattg tgacagggtc acaaatcccg ctggctgagt tacgctctga cggacaaatt
    24121 aatctgctga atgcgttgta cgttgcggcg aattatccta tcaacgaagt aactctcttt
    24181 tttaataatc gactgtatcg tggcaaccga accaccaaag cacatgccga tggttttgat
    24241 gcgtttgcct ctccaaacct tcctccgtta ctggaagcag gtatccatat tcgtcgtttg
    24301 aatacgccac ccgtcccgca cggtgaaggg gcattgatcg ttcatccaat cacgccgcaa
    24361 ccaattggcg tagtgacgat ttatccgggg atttctgctg acgtcgtgcg caattttctg
    24421 cgccaacctg tgaaagcatt gattctgcgc tcatatggcg tgggtaatgc gccacaaaac
    24481 aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt ggtcaacctg
    24541 acacaatgta tgtccggtaa agtgaacatg ggtggttatg ccaccggtaa cgccctcgcc
    24601 catgccggcg ttattggcgg tgcagatatg actgtagaag ccacgctaac caaactgcat
    24661 tacctgctta gccaggaact tgatactgaa accattcgca aggccatgag ccaaaacctg
    24721 cgtggcgaac tgacgccgga tgattaagga gactgcaatg ccccctcgcg ccctgttact
    24781 ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg aaggtgacag
    24841 tacggtggat gtcgctaacc gcctgattga ctggtgccag tcgcgcggtg aagcggttat
    24901 cgccagtcag gactggcacc cggcgaatca cggcagtttt gccagtcagc acggtgtaga
    24961 gccttatacg ccgggccaac tcgacggttt gccacaaacc ttctggcccg atcactgtgt
    25021 gcagaacagt gaaggcgcac aattacatcc gttactgaag caaaaagcga tcgcagcggt
    25081 gttccataaa ggcgaaaatc ctttagttga cagttacagt gccttttttg ataacggccg
    25141 tcggcagaaa actgcgctcg atgactggtt gcgcgctcat gtaatcaatg aattgatcgt
    25201 tatgggtctg gctactgact attgcgttaa gtttaccgtg ctggacgcgt tacagttagg
    25261 ttataaggtg aacgtgatta ccgatggttg tcgtggcgtg aatatccagc cacaggacat
    25321 tgcgcacgcg tttatggaga tgtcagcagc tggggcaacg ctatatacgc tggcagactg
    25381 ggaagaaacg caggagtaat tttacgctgg cctacaattc tgtactggca ttgtaggcca
    25441 gataaaacac atcagtgata catctggtaa ttgacgccat caacgaaaga tttaattcac
    25501 ctcagaaatc tcttccagcg acaccttccg cgtttcgatg ccaaaaatgg agagaaccag
    25561 cgcacagagc actaacatga caccaagcac cataaacacc gtaatcgacc cataatgtgt
    25621 taataatgcc gcaacgccat agggcgtgaa gactgcgacg atccgtccga cggcattaac
    25681 gaaacccgaa ccacgcaggc gtaaatgcgt tggccaaagt tccgggatat aaaccgccga
    25741 cgcgaagcac acatacatgt ataaaaagaa gatcatcacc aggccataga ttaaaatcgc
    25801 ccactctgta gtttgaattg aatagatata gcctaacaca gcaataataa taagtaaggc
    25861 agagccaaat aatcgacgag gaaaatgatc aataattaat gccgcaataa atatccctac
    25921 cggagcgcca atcataataa cagcggtcat taatattgat ttatcaacat caatgccgga
    25981 gttaacaaat atggtcggta tccatacggt gatggtataa agcgaaatgt tcattgcaat
    26041 taaaacagta atcgcgacta atgtacgtcg taacatttcc cctttaaaca gcagccagaa
    26101 ggtacccttg attaccttaa ccttgctgtt gctctggttc aaagtaagcg ggggtaaacg
    26161 aatactcttc tctctttcaa tttgctgctc tacttcacga agttggcttt ctgcacctgc
    26221 gatttgccct ttccctgcca gccatcgtgg cgactcaata aagtatttac ctgagagaaa
    26281 ccaggctaac agtatgccaa taccgcccag cagaaacatt atccgccaac taaaaaaagc
    26341 gataaccacc acgcctatcg ccgcagacag catgggcgac cagttaccaa caaatgagag
    26401 ccgcgcggac cattttccac gcaccgtcgc ggggataaac tccgtaaatg aggcatagcc
    26461 aaccataatc agcgccccca ttcctgttcc catcaggaag cgaaagaaga tgagccagta
    26521 catatcaggg acaaaagcgg cccctgttgc cgcaataccg acgatgagaa gatttatgcg
    26581 aaacgccctg cgccgcccaa agtagtcacc aataaaccca ccggtaagag agccgatgaa
    26641 ataaccaaac attaatgccg aggtaaaggc ggcattgagg aaattatttg accatccatt
    26701 gcttaccagc tttgctaaga cgacattacc ggagtaactc aaaaaccccg ttaataacag
    26761 actaaagctt attataccaa atatacgata atgaaaccgg gccaaaggca agcggtccag
    26821 tcttgcgcca atttgatcat attgttccat tgggtgcctc ggatttgcta aaaaaggtag
    26881 caattgcggc aaattcactt gccgcatctt tatcatcatt ttatttccag gcgcaggaac
    26941 aaataatatt gacaatgtgt gcttaaatta ccacaccagc tggatattat tgtctttgca
    27001 aaatgctatc cactctgcac ccggtgattt atcagttatt atgtaattaa tatgactaaa
    27061 gtcagctaac tggacaaaag ctttgcgatc aaacttagag tgatcaacca ataacgcaac
    27121 ttctgtcgcc tgacggatca ttgtcttttt gatttcagct tccgcttcat tagagtccag
    27181 cgcgccgctg ttaatatcaa gacctttaca actcattacc atgatatcga catggtagcg
    27241 gctgatgatc tctttagtaa ttcttccttg cagtgaaagt gtatttttgt ttagttcccc
    27301 acccgttgaa acgactttaa tttctgactg agccagaaca tgaatcgctt ccgccgagtt
    27361 ggttagcaac gtcaggtcac tacggtcctg taacaatttg agcaattcca ttacggtact
    27421 actggaatcg gctgccatgg tggttttatt gtcgataaag ggtagtgcct tgcgtgcaat
    27481 aagctgcttc tcttcataaa acgatgaagc gcgcttataa aaatggatat tctccgtcaa
    27541 catcgctgta tttaaaacag caccaccata ggttctggtc aaaaagcctt catcttccaa
    27601 cttctcaaga tcgcggcgaa tggtttcttc ggttacctga aaaatcccac tcaaatttga
    27661 gactgtcacc tttttatcgt tggcaaccat ttgcttaatt gcctgaatcc tgtcttttgc
    27721 cgccacgatt acacccctgt atctttttac atcacattag cgcaattatc gcataaccga
    27781 tgtttacttt caaaataacc tgtttgaatc acagattttc atcacagttt tcacagaaac
    27841 agaggtgaat cgtgttgagt atttaacgct ccagggcctc tgccatttcc ctcatcaatg
    27901 ttgcgtctgc atccgataag ttgatattca gtgccgcgac attttcacgt acctgttccg
    27961 gcgcagtagc cccactaaga atggagatta aatcactctg ttttaatatc cacgccagtg
    28021 ccagagtggg aattgtgcac tgataacgag cacagagtgg ctgccactgt tcaagcatat
    28081 caatcacttt cagcatgttt tcacgctgga accagacttt atttgcccga gcgccgcccg
    28141 gaacgtaatc acgcgtgatg gtcccggtca acaatccctg ctctagcgga gagtagacct
    28201 gtaccacaat gccattatca cgacatagcg gcagcagttc gttttccatt gcccggtcga
    28261 ggatactgta tttcgcctga ataatatcca gttcaccatg ttgcagatac tcgcggatat
    28321 ggtcagcatc gacgttagca gcgcctatag cgcgaatttt cccttcggcc tttaacgcat
    28381 taagcactgc gacagtttca gctatcggcg taaaaaatgg cggcaccgac tgccagtgcg
    28441 tcatgtagat atcgatgtaa tcaataccca gacgttgcaa gctggcttct acctcttcgc
    28501 ggatagattc cggagaaagg tttttataca actgccgatc gccaactttg ttgaataaac
    28561 tcccttttcg ttcccagaca atgccgcatt tggtttctac tacaacctgt tcacggggca
    28621 gtttttttaa cgcctgacca acgataactt cactattgcc aaagttatat cctggcgcag
    28681 tatcaatcag gttaatgccg caacgatggg cttcaagaat cgtatcaatg catatttgtc
    28741 gatcgagatc gccattccat gcaggaccgc cgccaatggc ccatgtcccc aaccccattc
    28801 gcgaaagcgt tatatccgtt gtgcctaaag gtatcttttt catttgcctt cattatcctt
    28861 cgtattcttc cagcaattgt tcgaccagct ttctgttttt tacaccggtg gtggcgccga
    28921 tgcttagaac cgagatagcc gccgttgcat tggcaaagcg tgcgcattca cgcagatttt
    28981 taccttctaa cagtgctgca ataaaacctg aagcaaagtt atcgcccgca ccaatggtgt
    29041 cgatggcggt tattcctgcg accgccggca ccttcatcgt catgtcacca cgcttgataa
    29101 agcagccgtc tttacccgtt ttaatcacca ccgtttttac gccgcacgca agaaagcagt
    29161 cagcaatttc atccagtgtc tctttcccgg tgagtaattt tgcctcggca aaattaggaa
    29221 acagataatc gacataactc aatgcttcgc aaatatcatt cagcgtttca ttcaaccgcg
    29281 gtttgatcat atcggcacag ataatcatct gccgggcttt agcttgcgta aaaatttctg
    29341 ttagcgctct accatccaat agaggactgt tgaaaatact ggccagtgat aataatttcg
    29401 cctgagaaaa ccgcgcaaaa tcaacatcgt caatattcaa tttccacaga ctgccgttac
    29461 ggtttgtgac aaaggtccgc tcgccatcct ccgtcaccag tccaacgtta atagaggtat
    29521 ctatgctgac gtcctgcttc aggctttgaa tatcaatatt ctctttgcgg caatggtcga
    29581 gaataaattg ccctgcggca tctttaccaa tacgactcat taatgctgtg cgatggccca
    29641 gacgagaaat aattgttgct tcatttattg catccccccc ggtggtcatt gcgattcttt
    29701 caagaggata agaatccaca tcaaagatat ttttactgac cggttgcaat ggaatatcaa
    29761 caatagcggc acctatacaa ataacgtcga gattatccat attattattc cgctttgcca
    29821 tcagaaccga acagtttaat tttatccaat gcccgttctt ttacagcttt acgcacttca
    29881 cgttctaaat gcaggaaagg ctgatcctgg ttttctttca ctgctaccat tgcggcctga
    29941 cagagctccg tatgaatgtt gattttcgca atacccaacg aaattgcagt tttaatgtcg
    30001 gcatcactga tccctgatgc gccatgcaaa accagcggaa cagaaacagc atcgcgtacg
    30061 cgtttgacga cctcaaagtt caattgtggc tctgacgtat aaaccccatg ctggttgccg
    30121 atggcgacag ccaatgagtc acagcccgta cgttcaacaa attcagccgc ctggtcagga
    30181 tcggtatagt gatagcccgc cagcgcttcc tcataaacgg tttcattacc aacatgtcct
    30241 aattccgcct ctaccggaat acccagcgga tggaagaaat caacagcctc tttggttaaa
    30301 cgaatatttt cttcgaaatc aaatgcagaa gcatcacgca ttaatgaatt cataccatga
    30361 gtccaggcgt tatgaataat ctccatactc cgaccatgat cccaatgagt tattaccggc
    30421 accgttgctt tttgtgccat tgataccatc atgtgagaga aatcttcaaa tgaggtgttg
    30481 ccgacaaaac ctgtgccaaa agaaataata accggtgatt tcgcttcttc ggctgcgtcg
    30541 ataacgccca tcaacatttc tgcattccat acgttaaaat gggcaattgc ataatgttta
    30601 tttgtggcat cgttttccca atatctgata tctgcgagca tgttctgttc tcccgtatta
    30661 attaatcgct aattttaata acgcctttaa taatgtcgcg tttgttgtta actgactctt
    30721 caaatgcctg ttgtacatcc cgataatcgt aaatatgcgt caccatcgat ttcacatcga
    30781 atcgccctga agaaatagct tcaatcgtga ccggataacg attggcatag cggaataccg
    30841 tctggatagt gacttcgcga ttgattttga ggaaattgat tgccgaatcg ccgggtacag
    30901 taccaacaat cataatttta ccgccgcgca tgaccagata aggtgcctgt ttaacggtga
    30961 ccgcagaacc cgctgtttca aaaacaatat ctgcgcccat gtcttcggta aattgctgac
    31021 agcgtgcaat agtgtcttct tttccgccgt taataaccac tgtcgcgccg agctgttccg
    31081 ccattgtcag acgtttttcc agcacatcaa cgacggcaat ttccgttgct cccaggcatt
    31141 tgcacgcttg caacgtcatc aaaccaatac aacccgctcc cagaataatt atcttcttac
    31201 caggtttaac atctgccagc atcgcggcat gcatcccgac tgcggcaggc tccaccagcg
    31261 ccccttccat cgtgtccata ttgtctggca gtttgtaagt aaagctctcc ggatgacaca
    31321 gatagtgcgt taatgcgccg cggtagttgg gttgtgtcgc cataaaatca acgtccgggc
    31381 aaatgttata tttgccttcc agacagtaac gacagtgacc gcaaggaacg ccaggttcga
    31441 tatttacccg atcccccggt ttaaatttgc gcacgcggct tcccacagcc acaaccgtcc
    31501 cggcgcattc atgacctagg ccaatttctt gatttgggtc tttaggcgga ataaacgggc
    31561 ctgattcaaa accatgtaca tctgaaccac aaataccgac atattccact ttaatcaaaa
    31621 cttcatcttc tttaggcaca ggtatttctg ctgaaataat tttcattgtg cccggcacct
    31681 gcaatattgc ttttgaattt ttcattttga atccaccagt attatttatt ggctactgca
    31741 tcaatactta aactttcaac ggagacacct ttggtttcaa ttccaatggt ggcgatagca
    31801 attgcgacaa taattgaaac agcccccaga agaataaaga ctcccgttac gccataacta
    31861 ctgagcagca ctgcaacggc ataaggcgcg gcaataccac tgattcgccc taccgcattc
    31921 gccagaccgg agccacggag tttggcctct gtcggccaga tctcagggac atacactgcc
    31981 gaggcatagc aaacgtacat atagacgaaa gtaatcagga agaaaccaat cagcgttatg
    32041 agcaacatac tggtttgcag tgaatagata tatccgagca ccgcaatcag gattaatagc
    32101 cccacaccca ttgttttacg cggaatttta tccatcacca gcatggcaat aaaaatacca
    32161 aatggcgcgc caaacatact catggtattt aaaacaatcg agtcttttaa attaattccc
    32221 tgggtcatga atatcgttgg caaccagtta attagtgtgt actgcacaac gttcatggca
    32281 atcagcacac aagagcctaa tatcacgcgt ttcagtaata ctcctgtcag taaggctgaa
    32341 tacggcaccg cttgtggtgc tttaccgtca tcagcaataa ccacaggcgg caaaggttta
    32401 cccgtctggc gtatgacgcc ttcttctata ctccgcatga ctttctcggc ttcctgataa
    32461 cgtccgcgcg attccagcca gcgcggggat tcaggaaagt agcgccaggc cagcgccgta
    32521 gcgataagtg acaatattgc agggataagc agttgtaccc gccagttcca ctctgcacta
    32581 atcagcggcg tgagtcccat cgcaatcaat gaacagagcg gatatgacca gttaccaata
    32641 aaggaaaccc gactggacca cgttccgcga tttcttccgg gcatatattc ggtgaaacca
    32701 gcaaacagtg taaccagtaa agctcccagc ccaacgccca tcacaaaacg gcaggcaata
    32761 aggaaatcca tattcggtga gaaagcaccg acaaccatcg aagcaatatg aatggcctca
    32821 tagaggatga acgcatttct gcgaccggtt ttgtcaccga tgatgccgcc caccagcgca
    32881 ccgagaaaca ttccggcggt cgtgattgct gagaatgtgg cagtggtgga attatctgtc
    32941 cagcccaacg ctttcagctg cgcgaggatc aagccaccaa cggcattact ccagcagaca
    33001 agcaagccaa acgcgacgat ggcaaacatt gacgaatgcc agcggcaatc cggtaagcga
    33061 tccagccgtg caccacaatg cggttttgtt atctgttcca tgcgataacg ttccttcagg
    33121 agagtagtta ttcatcaaag tcgtaagtca tgatcacttt gattgcagtt ttatcgacca
    33181 tcgcatcaaa cccttcgcgc cattgcgaca ggccgatacg atgcgtgatc atcggtttga
    33241 ctttgatcgc gccgctggct aatagcctga tagcgttacg ccatgaggtg gagtcatagg
    33301 ccatatgccc aatgatgctt ttgttccagg cggtaatgtc attaatcgag aaatcaagag
    33361 gtttgaagcc cattccaacg cgtaccactt ccccgttcgg acggagcatt tcgatggctt
    33421 gtttcagtgc gatattggca ccggagcatt caatcaccag tcccagattg tctttgccac
    33481 aaatttgctg gcagcgcgcc accacatctt cggtagaacc atttactact gccgtcgcac
    33541 ccagttcttt tgcaaccggg aagcggaccg ccacatcttc ttgtagacca acgacgacga
    33601 tatttaccgc ccccataatc cgcgccattt gtacggagaa cagcccgagt gggccagtgc
    33661 cgatgacgac cacatcctga ccagggagga atttcgattg ctgcgcgatg gatttgtagg
    33721 cattacagat agggtcaagt acggctgcgt cctcataatc aacaccatca gggatttccc
    33781 acaacgcatg acgatgaatt ttgagaattt caccaggaac aagacaatat ttggaaaaac
    33841 caccgcccca ggtattgtta tccagaccaa ggtttacttt ttctgtacaa cacagaaaat
    33901 caccttgttc acaggccggg caaacaccgc aaacgtgacc gctgttatcc gacacgacgc
    33961 gttgccccac tttccagtct ttgacttttt caccaacctg cgcaatacaa cctgcgaact
    34021 catggccgcg gatagagtta aactcatcag aaccgctatc gacattgtag tgcttcatgt
    34081 ctgcgccgca gattgccgcg gctttaattt caatcacgac atcttccggg ccacaaatgg
    34141 gttgtgggac atcaatcatc ttgtagccgc caaaggcctt gccaaaccga gccagtgctt
    34201 tcattgcgtc atcctctgtg ttttgttttg cttagatggt catcatactt acccacatga
    34261 tttttgaggt caacatcaaa tctgtaaaac acagataatg atctgcgttt tacaacttgg
    34321 atcacaatta cgcagaaaat ggctggggct gttacaggtt gatggaaggc gggacgcaaa
    34381 aagagcaaat tcgagaggga ttcccggcca aataggcctg agacttaagt tcaggagaag
    34441 tcattcaggt gtgaacgcga ctgcaagaga tcggactggg acttagccag gtcggggcga
    34501 gataattgag atgagtcgtg gggtcattgt tggctatccc ctttataacc gcggcaaaaa
    34561 ggggataaac cacttactta aacatggcaa taggctttgc tgagatacca aaatcttctt
    34621 ttaactgctg tttgctcttc atgaccatct gaccatttgt gtcaattgtc atatgctgtg
    34681 cttcggtatt atggcgggct tgccacagca tcaccagttg caggcagttc tctttttgtt
    34741 cctcggtcaa cgtaacgcca tcaggccatt tccccagttc aacggcggtc gacaaacgct
    34801 ggtatacttc aggcgtcatg ctgttgataa tgtcatcaag attcataatt tttccgctcc
    34861 tgtggaataa gttgctgaat cgttttttca accgttgatt tcttcgccgt tttcgccatc
    34921 agtgaagcgt aaagaagcag agttaacaca ataacgttcg cccgttggct gcggcccgtc
    34981 agggaagaca tggcccagat gggcatcgca gttaccgcaa cgaatttcta tgcgctgcat
    35041 tccatgtgac aagtctttga tataacgaat agattcttca ctcaccggtt cgtagaaact
    35101 gggccagcca cagccggaat catacttggt ttgggaatga aacagcgggg catcgcagat
    35161 caaacagtga tatacgccgt cacgcttgtt atgcagtaaa cgacccgtaa atggcggttc
    35221 tgtcccatga ttctgcgtca cgtagaactg catctcggac aaattttttt tcagttcttc
    35281 tgccgaaggt ttattagcca tttgctcaca tctcacttta atcgtgctca cattacgtga
    35341 ctgattctaa caaaacatta acaccaactg gcaaaatttt gtcctaaact tgatctcgac
    35401 gaaatggctg cacctaaatc gtgatgaaaa tcacattttt atcgtaattg ccctttaaaa
    35461 ttcggggcgc cgaccccatg tggtctcaag cccaaaggaa gagtgaggcg aatcagtcgc
    35521 gtaatgctta ggcacaggat tgatttgtcg caatgattga cacgattccg cttgacgctg
    35581 cgtaaggttt ttgtaatttt acaggcaacc ttttattcac taacaaatag ctggtggaat
    35641 atatgactat caaagtaggt atcaacggtt ttggccgtat cggtcgcatt gttttccgtg
    35701 ctgctcagaa acgttctgac atcgagatcg ttgcaatcaa cgacctgtta gacgctgatt
    35761 acatggcata catgctgaaa tatgactcca ctcacggccg tttcgacggt accgttgaag
    35821 tgaaagacgg tcatctgatc gttaacggta aaaaaatccg tgttaccgct gaacgtgatc
    35881 cggctaacct gaaatgggac gaagttggtg ttgacgttgt cgctgaagca actggtctgt
    35941 tcctgactga cgaaactgct cgtaaacaca tcaccgctgg tgcgaagaaa gtggttatga
    36001 ctggtccgtc taaagacaac actccgatgt tcgttaaagg cgctaacttt gacaaatatg
    36061 ctggccagga catcgtttcc aacgcttcct gcaccaccaa ctgcctggct ccgctggcta
    36121 aagttatcaa cgataacttc ggcatcatcg aaggtctgat gaccaccgtt cacgctacta
    36181 ccgctactca gaaaaccgtt gatggcccgt ctcacaaaga ctggcgcggc ggccgcggcg
    36241 cttcccagaa catcatcccg tcttctaccg gtgctgctaa agctgtaggt aaagtactgc
    36301 cagaactgaa tggcaaactg actggtatgg cgttccgcgt tccgaccccg aacgtatccg
    36361 tagttgacct gaccgttcgt ctggaaaaag ctgcaactta cgagcagatc aaagctgccg
    36421 ttaaagctgc tgctgaaggc gaaatgaaag gcgttctggg ctacaccgaa gatgacgtag
    36481 tatctaccga tttcaacggc gaagtttgca cttccgtgtt cgatgctaaa gcgggtatcg
    36541 ctctgaacga caacttcgtg aaactggtat cctggtacga caacgaaacc ggttactcca
    36601 acaaagttct ggacctgatc gctcacatct ccaaataagt tgagatgact ctgtgatcta
    36661 aaaagagcga cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt
    36721 catgattaag aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg
    36781 tcgtaaactg gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt
    36841 tgcattacag ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aaattctgtg
    36901 gttaagtaac aacacaccgt tcaaaaatgg cgtcgccatt cgcggtggca taccgctttg
    36961 ctggccgtgg tttggtccgg ctgcacaaca aggtctgcct gcgcacggtt ttgcccgcaa
    37021 cctgccgtgg acgctgaaat cacatcgtga agatgctgat ggtgtagtgc taacttttga
    37081 attgacacaa agcgaagaga cgaaaaaatt ctggccgcac gactttacgc tgttagcgca
    37141 tttccacgtg ggtaaaacct gtgaaatcga tcttgaagca cacggcgaat ttgaaactac
    37201 ctctgctctg catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtggact
    37261 aggcgatcgc ttcattgata aagtaaatga tgcgaaagaa gatgtactga ccgacggtat
    37321 tcagaccttc cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtaat
    37381 taatgacgaa gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt
    37441 aggctggaac ccaggaccgg cgctttcagt tagcatgggc gatatgccgg atgatggcta
    37501 caagaccttt gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga
    37561 gaaacctgca catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatatt
    37621 gtgtgccggg tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt
    37681 tccttttggt gccggatacg ctttatccag cataactaat tccgctgacg aaagttcaac
    37741 ctcaagcaca gccgcatttt gttggacatg tgcgacagtt gccgcttttg gaatcgccat
    37801 cacaccctga tggctgatta cccacgccaa caatacttgt gccgcactga tattgtgagc
    37861 atgtgcaatt tcgttgacta ccgcattttt taacagtcca ttgcgcaacc gcccggcctg
    37921 ggctaatgga ctgtaagcca tcaccggcat ctgctgtttc tggcaccagg gaagtagatc
    37981 gtactcaatc cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc
    38041 ccccggcagc tgccagagtt cctgcatatc ggcataatca aggttagaaa cgccccagcg
    38101 gcggattttt ccctgggtga tcaatttttc catcgctgcg acagtctctt cgagagcgaa
    38161 actgccagac cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact
    38221 ggcttcgcat gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac
    38281 gagaaagacg ttatcacgca gaccgattaa tgcttcccca accacttttt cggcaccgcc
    38341 atcggcatac atttcggcgg tatcaatgag ggttaaaccg agttcaatgc ccgcgcgtag
    38401 tgcagcaact tctgttttac gctgactggc atcttcgccc atataccatg ttccctgccc
    38461 tatggctggc agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgcttcctc
    38521 ccaggtattg caccaccgta atgcaaaaca gggcatgacg ccctgttttt atgcacaaaa
    38581 tgccctggaa aaatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc
    38641 caggatttat ccaccattgg gctgtcagta acttcatcag acagacgggt gtagcgcgcg
    38701 gtaccgtaaa cactccagtc accgaggaag ttgtagctgg cgctcagctc caggtaaggg
    38761 ctccagctgt cgttcgggtt atagccacgc agcccgctgc gagcggactc tttgcgcgat
    38821 acgccataat agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc
    38881 acggtcaggc caccgttggt gtaacgatac aaccaggcca tatcccagac gatgccgttg
    38941 ctgttatcca gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga
    39001 gcataagaca gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga
    39061 tcgccactgt ctttagcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagtttg
    39121 tccgttgcgt cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg
    39181 ccttcatagt tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt
    39241 tcaacgacac ctacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca
    39301 gacgttgcga taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc
    39361 ttaaacaaat gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt
    39421 ttacgttttc attttttata gtaactgttt aattttcctg acgcagatga caccacgctt
    39481 acagccaaat gaatttagcg ttaccggcga ccctattatt tacattaatt atgcaaaatt
    39541 tatggatgag ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat
    39601 ttgccatcta cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata
    39661 aaatggcatg agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg
    39721 agcctctact attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata
    39781 ccatgaatat attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca
    39841 cactgcagga gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg
    39901 agcggctatt gatggctatt ggtgagcctg tcatggtcga tacagcccag gaacccagac
    39961 tttctcgact cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg
    40021 gcatggaaga cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg
    40081 aagagaagaa acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg
    40141 ctgagcgact gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg
    40201 agcgtagccc ggtcaacgat catcctttct gtcttttcaa tccgcaggaa gatgcgcaga
    40261 ttctggaaaa agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg
    40321 cggcaaagcg cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct
    40381 ggccgtcaat tctgcaacaa attgccatcg ccaaaacgga acccggcgat gagaacaacc
    40441 aggacatctc cgccctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga
    40501 atgacccgga cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg
    40561 aatttgtcga gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccaccc
    40621 aggaaggtaa ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc
    40681 tcgcccactc gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct
    40741 tcctcgaccg tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca
    40801 aaatctacga gaaattgctt aatcacagtg aattgactca cgccccctgc gcccctggca
    40861 cgctggaaac gctgtcacgt ttttctattc tttcgcgtct gaaagagcca gaaaactcca
    40921 gcatttactc aaagatgcgg gtttatgatg gtgaaagttt gaaagacact gatcccaaag
    40981 ccaagtcgta tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt
    41041 cgacgcgttt tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg
    41101 cggcaaaccc ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc
    41161 cgcaagagca ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg
    41221 ccgagtttat cggcaaagag atccagacgg cctaccttga atcctattca gaatatgggc
    41281 agaacatttt tgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc
    41341 gcgatccgga taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa
    41401 tcgagaaacc ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg
    41461 tactgcgagc cagagccaat aacagcggac gtaatccgaa ctggaccagc tatgaaaaac
    41521 tgcgcacggt tattgagaag aaaatgttct ctaataccga ggagctgttg ccggttattt
    41581 cgtttaacgc caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta
    41641 tgatggaaaa aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg
    41701 tacgtaaatc gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag
    41761 cgaaccgtgg gccagagaag cggcaacaca ggcgtagcat accgttggca aatgtagtac
    41821 ggggggcata tgacctggtt tattgaccgg cgtctgaacg gcaaaaataa aagcatggtg
    41881 aatcgccagc gtttcttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc
    41941 ataaataagc gttcggtgac tgacgtcgac agcggcgagt ccgtatccat tcccacggaa
    42001 gatattagcg aaccgatgtt tcatcagggg cgtggtggcc tgcgtcaccg cgtacatccg
    42061 ggcaatgacc atttcgtcca gaacgaccga attgaacgtc cccagggtgg tggcggaggt
    42121 tccggcagtg gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt
    42181 cagatttcga aagatgagta tcttgatctg ctctttgaag atttggcctt accaaatctg
    42241 aaacaaaacc aacagcgcca gttaaccgaa tataaaacgc atcgggcagg ttataccgct
    42301 aacggcgttc cagccaatat cagcgttgta cgttcattgc agaactcact ggcgcggcgc
    42361 acagccatga cggcgggcaa gcggcgggaa cttcatgcac tggaagagaa tttggccatc
    42421 atcagcaaca gtgaacctgc gcaactgctg gaagaggaac ggctgcgcaa agagattgct
    42481 gaattgcgcg ccaaaattga acgcgttcct ttcattgaca ctttcgattt acgttacaag
    42541 aactacgaaa aacggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt
    42601 tccggttcaa tggatcagtc cactaaagat atggctaaac gtttttatat tctgctgtat
    42661 ctgttcctca gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag
    42721 gcaaaagaag tcgatgaaca tgagtttttc tactcgcagg aaactggcgg caccattgtt
    42781 tccagcgccc taaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg
    42841 aatatttacg cagcccaagc atcagacggc gataactggg ccgatgactc tccgctttgc
    42901 catgaaattc tggcgaaaaa aatattaccc gttgttcgtt attacagcta tatcgaaatt
    42961 acacgtcgcg cgcatcagac actgtggcga gaatatgagc atctgcaatc tactttcgac
    43021 aactttgcga tgcagcatat ccgcgaccag gatgatattt atccggtatt ccgtgaactg
    43081 tttcataaac aaaatgcaac agctaaagac taaaactgtc agccgggtca ttatcgcctg
    43141 gctgattttt agctcactgt aaattatctc atttattaca tacattagct tacaatcgct
    43201 ttaaatatga cagcataacc tttacacaat ttagttccag aaaacaatca ttcggaaaaa
    43261 tgattcggtc aacacgtatt tccctggggt tattctttaa ttatttttta tcgttaacga
    43321 aaattgatcc tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg
    43381 ctctcctttt tatggtttct atgggtacac aaaaattaaa agctcaaggc ttttttattt
    43441 tcagcttatt gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa
    43501 atgtaaaact catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta
    43561 acgccgtcat tttccttttc atgttaatga agtatttcac taacaaacaa attttaccaa
    43621 cactcatttt aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa
    43681 ttatccataa accaatcaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta
    43741 ttttctatat tttccgccaa ctcagtttca tttgtttaac ctcgctggcg ctcttttgtt
    43801 atgggaaaga caacatcctt gacaacaata agaagaaaac tggcattctg ttgctggcgc
    43861 tgattccttt tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact
    43921 attctttgta tgtcgtcgat tactgtccgg acaaccatac tgcgacctgg ggaatcaact
    43981 atacaaaaat attggtttgt ttgtgggcat ttttactgtt ctttattatt atgcgtacac
    44041 gattagccag cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca
    44101 acttacttct actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga
    44161 ttgaagtttc cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggaac
    44221 tgcaactctc cagcaaactg gcagttcatg atgtactgac caatatttat aatcggcgct
    44281 actttttcaa cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttttgtgtca
    44341 tgctggttga tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg
    44401 ataaagtgct ggtatcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt
    44461 tagcgcgact ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg
    44521 caaaaatcat tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca
    44581 gatatgatgt tcctgaacaa atgacaatca gtattggcac ggttttttca acgggtgaca
    44641 cgcataatat ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg
    44701 aggggggcaa caaagtgatt attcatcata tttaagtgca aaaatattca cagccatgct
    44761 ttttgcgtgg cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg
    44821 gcgcagagat gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt
    44881 ttctcgtttc actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc
    44941 ggcattttat cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg
    45001 tcgccagcga cagagcaatg agtgcctatc tgcgctatat cgtgcagaga gcagactctt
    45061 cctttcttta tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg
    45121 ctgctgagca gtcggaagtg ccgccagaac agcggcgcgc catctgcgag gcttttgagt
    45181 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
    45241 cattacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
    45301 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
    45361 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
    45421 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
    45481 ttggtatcga tcgcaaagat attgaaaaag tatttaccgg gcgtaccgta ttatcgagca
    45541 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
    45601 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatctg cggaatatct
    45661 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgatacag
    45721 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
    45781 acgtccatga cttaccgggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
    45841 tcacgtcttc gtggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
    45901 tggtgcggat gcacttccgc ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
    45961 tgactggact ctataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
    46021 tggtgcaatc cggttcttcg gtgatgttta ttgctattga catggacaag ttaaagcaaa
    46081 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttatta gctcaggcga
    46141 ttaaacaatc gattcgtaaa agtgattatg ccatccgact cggtggcgat gaattctgca
    46201 tcattcttgt cgattcgacg ccgcaaattg cagcacaact gcctgaacgt atcgaaaaac
    46261 gtctgcaaca tatcgcgccg cagaaagaaa tcggcttctc ttccggtatt tacgcgatga
    46321 aagaaaacga tacgttacat gatgcgtata aagcttccga tgagcgttta tatgtcaata
    46381 agcagaacaa aaacagccgt tcatgataac cttctgtagt tgtttgcttg taatctcagg
    46441 agcatgaaat gactgaaatg gctaaagaaa gcgtgacgca tcagcggtta atcgctttat
    46501 tatcacaaga aggtgctaac ttccgcgtcg ttacccatga agcggtaggt aaatgtgaag
    46561 cggtgtcaga aattcgtggc accgctttag gccagggggc taaggcactg gtatgtaaag
    46621 tcaaaggcaa tggcgtaaat caacatgttc tggcgatcct cgctgccgat caacaggccg
    46681 atctgagcca actcgccagc catataggcg gattacgcgc ttcgttggcc agcccggcgg
    46741 aagtcgatga actcacaggc tgtgtcttcg gcgcgatccc ccctttcagc ttccatccaa
    46801 aactcaaact ggttgccgac cccctactct tcgaacgatt tgatgaaatc gccttcaatg
    46861 caggcatgct ggataaatcc gttattttga aaaccgcgga ttatttgcgt attgcgcaac
    46921 cagaactggt caatttccgc cgcactgcgt aactagcctg tccgttcaat aagcagaacg
    46981 gacaaaacca gcacagcagc aatggcaaaa aacgatgacg tgataatcag tgtctcgacg
    47041 aacatttgat cgttcatagt atgcccccgg gagatagctg ttttcacgtt attgttagcg
    47101 tgcacaaatg gcagtttgat gacagttcgc ctgatttttt atgcagaaaa cgtaaagatt
    47161 ttctacttcc tgcctgcagc aagcgtaaag taagcaggct tattattttt tggcaaggaa
    47221 accacgatgt ttgatgtcac tttgctgatc ctgctcgggt tagctgcgct gggctttatc
    47281 agtcataaca ccactgtcgc cgtttcaatt ctggtgttaa tcattgtccg cgtcacaccg
    47341 ttaagcacct ttttcccgtg gattgaaaaa caggggctaa gtatcgggat aatcattctg
    47401 acgattggcg ttatggcccc catcgccagc gggacgctac cgccctcaac cctgattcac
    47461 tcgtttctga actggaaatc gctggtggca attgcagtcg gcgttattgt ctcctggctg
    47521 ggtgggcgcg gcgtgacgtt aatgggcagc cagccgcaac tggtcgccgg gctgctggta
    47581 ggtactgttt taggcgtggc gctgtttcgc ggcgtaccgg tcggcccgct tattgccgcg
    47641 ggtctggttt cgctgattgt ggggaaacag tagttaatcc cgcaatatat cggcctggcg
    47701 tttgtcccag gcctttctta aacattgtaa taaaggccgt tgttgaatca taccccagcg
    47761 tatgagcaac tttctgtaca gtatcgcctt tcactaatcc ctgaagcgcc ataatcagct
    47821 gtaactgctg acgccactgg cgaaaactca accccgtctc tttcactatc agccgggcaa
    47881 gattgcgttc actcatcgca aaaaagccag cccattgccc caatgagccc cactcgacgg
    47941 gctctttcgc catcatctcc accattgtgc ggattttagg atgagaagaa acgggcaggt
    48001 gcagctgttg ttgtggctgc tgagggagtt catcaaacag gacctgaatt aatctgcggg
    48061 tcatcggctc agcacgctga gtcgtcgtcc tgtttgccag ggttaaaatt aattcccggc
    48121 ataagggcga tattttcaat gtgcaacatg ttgtcggcat tgtcactgcg ctgggctcga
    48181 taaacagaaa acagagttca gcgttagctg tcacttgatt gctatgtttc accccgcccg
    48241 gtatccaaac cgcatattgt ggcggcacca tccacaaagc attttccacc gtacaggtaa
    48301 ttgcaccatg tagtgccagt atcaactgcc ctttgcggtg ctgatgtaca ggactggtta
    48361 actcatccgt tccggcgtga atacaaaatg cgaccgcggc ttcatggtgt cggtcgggtt
    48421 catagccatt gagattcaac ctgtgcatca ttttgtccga atttagcgat attttgtcat
    48481 tttagcttga ttcaacataa caataaaaac ggtaaggtac agcctcgttt gtaacaatga
    48541 gaagcatatg acctgttcaa cttcattaag cggcaaaaac aggattgtcc ttatcgctgg
    48601 cattctgatg attgccacaa cattacgcgt cacctttacc ggcgcagcac cgttactgga
    48661 tacgattcgt tccgcttact cgctaacgac ggcgcaaacc ggcttattga ccaccctgcc
    48721 attattagcc tttgcgctga tctcaccgct ggccgccccg gtggcgcgac gttttggtat
    48781 ggaacgtagc ctgtttgccg cgttactttt gatctgtgca ggtatcgcaa ttcgctctct
    48841 cccttcgcct tacttattat ttggcggtac agcggtcatt ggcggtggga ttgcattagg
    48901 caatgtctta ctgccaggat taattaaacg cgatttccct cattccgtcg ccagacttac
    48961 cggcgcatat tccctgacaa tgggagctgc ggcggcactg ggatcggcga tggtagtgcc
    49021 gctggctttg aacggttttg gctggcaagg cgcgttgctc atgctgatgt gttttcctct
    49081 gctggctctt tttttatggc tgccacagtg gcgaagtcaa caacatgcaa atttgagtac
    49141 ctcacgcgcc ttacatactc ggggtatctt gcgttcgccg cttgcctggc aggtaacatt
    49201 gtttcttggg atcaactcac tggtctatta cgtgattatt ggctggcttc cggcgattct
    49261 catcagtcac ggctatagcg aagcacaggc gggttcactg catggtttgc tgcaactagc
    49321 cacagcagct cccggtttgc tgatcccact tttcttacat catgtgaaag atcagcgtgg
    49381 tattgcagcg ttcgttgcct tgatgtgcgc agtgggcgcg gttgggctct gctttatgcc
    49441 agcacacgcg atcacctgga ctctgctttt cggttttggt tccggcgcaa caatgatact
    49501 ggggttgacg ttcattggtc tgcgggcaag ttctgcgcat caggcggcgg cactctcggg
    49561 gatggcacaa tccgtcgggt atttgctggc agcctgtggg ccgccgctga tgggtaaaat
    49621 acacgatgct aacggtgact ggtccgtacc actgctgggt gttgcaatac tttcactact
    49681 gatggcgatt ttcggacttt gcgccgggag agacaaagaa attcgctaat atccggtgct
    49741 atagtgacgt gacaaatcat gcgtgaaagg gagaacaaat acgatgaata ttcagtgcaa
    49801 acgcgtttat gatccggctg aacagagcga tggttatcgc gtactggtcg accgcctctg
    49861 gccacgcggt atcaaaaaaa ccgatttagc ccttgatgaa tgggataaag aaatcacacc
    49921 gtcaacggag ctgcgcaaag cctttcacgg cgaagtcgtc gattttgcaa cctttcgcga
    49981 gcaatatctt gcagaactgg cgcaacacga gcaagaagga aagcggctgg cagacatcgc
    50041 caaaaaacag ccgctgaccc tgctctactc agcaaaaaac accacgcaga accatgcgct
    50101 ggtgctggca gactggctac gtagcttgtg attttagtac agcatccggc ggttattttt
    50161 caccagccgg atggtcccgt cgccacaatg cccattcatc aatcgtttca ccgcccggta
    50221 atttgcaatt gttgctgacc ccttgcgctg tctgcacagg aatgagcgtc ccgcccttct
    50281 gctggcaata gaccgacgcc ggatttgcca taccaatctg cggcggttta ggtgcttctg
    50341 gctgagaagg ggttgaacaa ccagccagga ccagcaagca aggcagaaca aaactgataa
    50401 ttttcattta ttgatctcac atatttatcc aggcttagag tatcgcggta tcgttttgtt
    50461 ttgcagcact atttttatta cattcactca aaacatatta cgccttgttt catctttgtt
    50521 gatgatgttt tatcatgcct gcaaagatta aataatcagc atttacccgc cgtatcctgg
    50581 agttgtttcg tgtcagatca gattatcgcc cgcgtctcgc aatcccttgc caaagaacag
    50641 tcactggaaa gtctggttcg acagcttctg gagatgctgg aaatggtcac tgatatggag
    50701 tcaacctacc tgaccaaagt ggatgtcgaa gcgcgcctgc agcatataat gtttgcccgt
    50761 aatagccaga aaatgcacat cccggagaat tttaccgtct cgtgggatta ctcgttatgc
    50821 aaacgcgcca ttgatgaaaa ctgttttttc agcgatgaag tccccgaccg ttggggcgac
    50881 tgtgttgcgg cacgcaatct tggcatcacc acatttctga gcacgccaat tcacttaccg
    50941 gatggatcat tctatggcac gctttgcgcc gccagcagtg agaagcgcca gtggagtgaa
    51001 cgcgcggaac aggtcttaca gttattcgcc ggactgattg cacaatatat tcaaaaagag
    51061 gcactggttg aacagctgcg cgaagctaat gctgcgctga ttgcgcaatc gtataccgac
    51121 tcgttaaccg ggctaccgaa tcggcgggca atttttgaaa atctgacgac gctgttttct
    51181 ctcgcccggc atcttaacca taagataatg atcgcgttta tcgatctgga taacttcaaa
    51241 ttaatcaatg atcgttttgg tcataatagt ggcgatctgt ttctcattca ggttggcgag
    51301 cgccttaata cgctccagca aaatggtgaa gttattggtc gtctcggcgg tgatgagttt
    51361 ttggttgttt cactgaacaa cgagaatgcg gatatttcgt cgctgcgaga acgtattcaa
    51421 caacaaatac gcggagaata tcacttaggt gatgttgatt tgtattatcc tggtgccagt
    51481 cttggcatag tagaagtcga tcctgaaacg accgatgcag acagtgccct gcatgctgcc
    51541 gatatcgcga tgtatcagga gaaaaaacat aaacagaaaa caccttttgt cacgcattca
    51601 gcgctacatt cctgaggcgt attcacatcc ttttgattgg tgataacatg cgaatcggta
    51661 ttatttttcc ggttgtaatc tttattacag cggtcgtatt tttagcatgg ttttttattg
    51721 gcggctatgc tgccccggga gcataaagat gaaaaaaaca acgattatta tgatgggtgt
    51781 ggcgattatt gtcgtactcg gcactgagct gggatggtgg taacgtcaca tctaaaaaaa
    51841 tagcaaaggc tgcctgtgtg cagcctttgt gcaatttacg cgttaacttt taatcttcct
    51901 gtagataaat agcacaacaa tcgcaccaat aacggcaacc acgaagctgc caaaattgaa
    51961 gccatcgact ttaccaaagc caaacagcgt gctgatccat ccgccgacta cggcaccgac
    52021 tatccccagc aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt
    52081 cgccagaata ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc
    52141 acttatctgt tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcggga
    52201 gggtaagatt tgtgacgagc atcacggagg aattttttgc gatggcgcag aaattgcgcc
    52261 atcaacgatc agtgataatt accaaccaca aacatcatgt tcgttttccg tgtcataaga
    52321 acgcacggta ttcaccagat cttttatcac ttcagccgcc acttctggca ccagcaaagt
    52381 catcggcgtc tctgtttcat aatcgacaga aacgccattg ctgttattgg tgacggtcac
    52441 ggtatacgtt gctttgccca tgattcattt cccgttatga atgactttcc gttgttgcgc
    52501 accttccatc aggacttcag gagcaacgaa gaagtcaatg ttgaaataag tatcgtcagt
    52561 catggcttca atgttgtgcc acttttctgg agggaacacc gcaaactgcc ccgcttcgat
    52621 aaggatcacc tgatcaggct ctgcactgtg ttcatcagcg tagccgagat atttgaccgc
    52681 cccatgcata acggaaaggc gtgggtaaac ccctgggcgc gttcctttat caagatgacg
    52741 ttcgaatatt ccggcaggcg cagtttgttt attccagaaa ggcgttgagc gcgtatgaat
    52801 ataattctgt gggatttgca gcatcctttt ccctccttcg gtgaatgcgc tgaaaacggt
    52861 ttattccagc cgtttcaggg tacgcctgat aatttgcatt ttaaatacca tttattggtt
    52921 actttttagc accgtatcag agaagaatca gggagaatta caggtgggaa gcccgcggca
    52981 acgcgggctg aaagcatcag gattgcagcg tcgccagtcg ggcggcgaaa cccacgaaca
    53041 tcaaaccaat cagtgagttg cccactttag ccagtttctt tttggtacgt atgtactgcg
    53101 tgacaaaagc cccagaaata atcagaaagc tcaaatagca gaaactcacc agttccagcg
    53161 tcgtcgccag aataaagaat gaaattcccg tatgtggggc attaacatcg ataaactgta
    53221 cgaaaaacga cacatagaac aaaatggctt tcggattagt caggctcaaa attaacgcgc
    53281 gtttaaaaat ggcaccgtat tggggctcat cggatttggt ctcgctattt ttacctttca
    53341 gggtcgcgta gagaatttta ctccccagat agagcaaata aaacgcacca agataacgta
    53401 caatgttgaa taatatcggg gtggtcttaa ttaatgtcgc cactccagcc catgccagaa
    53461 acatcaatac cgcatcgcca ataaatacac cacaggccgc aagataaccg cctttcatac
    53521 cgctactgac gctatttttg agtacaaaca gggtatttgg ccctggcacc aacacaataa
    53581 aaatggcccc aaccagatag gtccagtaat tcagaacccc gtattcagcg aacacattaa
    53641 cctctttaat tatctttcga tcatgcgcga ttaaaagtga atatgctaac caatctgtag
    53701 cggcttagaa aggagaaaat cagattttaa cccgatatca acccgataat tgaatcatta
    53761 atcaggcata ctccagtgaa aaattcgggt agtgctcggc aaaatactgg cgtaaaaatt
    53821 ctaccgttat ccgcactttc gctgacatcg ccagccttga aacataaacg gcccagacat
    53881 tcgctggctg gtaatattcg ggtagcactt gtactaaatg accactggcg ggaaacagcc
    53941 tggcgacatc gatttttacg tggtccggaa aaacaccgaa ggcgaatatt cctcgccagg
    54001 cggtagagta aatgaaggaa cagagaatga agtcgtcatt caggaatcgg tatttactcg
    54061 tcgtggtgtc gagcgcattt tgcgttaagc tcaaataagt tcatttgaac cgcctgctgt
    54121 tggatttgta gtttgccgaa gccggactga gcgcgcccag tccggcttcg gaagatttta
    54181 ctgtggatat tcctgcaata aattgtgtaa tgcttccgcc atcagctcac cacgccagcc
    54241 ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc cagtgccagt tcagcagttg
    54301 gttgatttgc cgacgcgatg ccagcaattc ggcgctgatc ttgtgcgttt cgctgacgtc
    54361 agtaatcagc gacttaatcg ctttaaacgc tttacgataa cccggcatgt ccatcaggtt
    54421 aagcatcggc tgcggtaagg cctcttccgg caatgcctgc gctttttcca ccagcgccag
    54481 caacgtttta ccgtggaagc ggatttcgct accggataag cccaggctgt ccagttcgcc
    54541 cagacttccc ggcatataac gcgctaccga ccacaaatgc tcttcacgca cgacaaagtt
    54601 caccgccaga tcgcgctctc gcgccttgcg caatcgccag tcggctaaca gttgcagaca
    54661 ggccagttgg cgtgtgcgta attgccaggc attggtgata tcacgccagg cgtcttccgg
    54721 cgcaacgact tcctgacgac gcatttgcat caggcggcat tcatccagcg ccgcaggtag
    54781 ccagccggag gcctccgttt ctaccataag ctttgcggtg atcggtaaca gataccagac
    54841 atccgccgct gcgtattcac actgacgttc ggtcagcggt ctggccagcc agtcggtgcg
    54901 cgattcactc ttgtccagcg taacgccgga atactcttcc accatggaag cgaaacccca
    54961 tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg tcaatcaagg gttgtggtaa
    55021 ttcgccaaag acattgagga acacttccag atcttcactg cctgcatgaa gaaattttgt
    55081 gatggacgga tcgcgcagga tcgctttcag cggtgaccag tcggtgatcc cgagaggatc
    55141 aattagcgcc agatgctcac catcaaaaag ttgaattaaa cccagctgcg ggtaataagt
    55201 gcgcgtacga acaaattcag tatccagggc tatcgccgga aaggcacgga cggcttcaca
    55261 caaagaagcc agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt attctcttta
    55321 gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac gactgactta acgctcaggc
    55381 tttattgtcc actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa tttttccgac
    55441 gttagatttc ggtaactcat cacgaaactc caccagcttc ggtactttgt agcccgtgag
    55501 ctgacggcgg caaaaggtca ccagtgactc ttcggtaagc gatggatctt ttttcactac
    55561 gaagattttc accgcttcac cactggagcc ggaaggtacg ccaacagccg cgacttcctg
    55621 tacgccagga tgctgcatga cgacatcttc aatctcgttg ggataaacgt taaaaccgga
    55681 aaccagaatc atgtcttttt tacgatcgac aatgcgcagg aatccttctt catccatcac
    55741 cgcgatgtcg ccggtgtgta accagccatt tttgatgatt tcatcggtag catccggacg
    55801 ctgccagtaa cccagcatca cctgcggtcc tttgacacaa agctcacctg gttgaccagg
    55861 tggtacttca ttatcatcat catccaccag tttggcttcc gtcgacggca ccggtaaacc
    55921 gatgctacca ctatgataat caatatcata cgggttaacg ctgaccagcg gcgcgcactc
    55981 ggtcaggcca taaccttcca gcagatactg tccagtcagt ttaacccaac gttctgccac
    56041 cacttgctga acaggcatcc caccgcctgc ggaaagatgc agactggaga aatccagctg
    56101 ctggaactct ttattgttca gcaacgcatt gaacaaggtg ttaacgccag tgatagcggt
    56161 aaacggatat ttcgctaact cttttaccag ccctggaata tcgcgcgggt tagtgataag
    56221 caggttctgc ccccccagtt cgataaacag caggcagtta atggtcaggg caaaaatgtg
    56281 atacagcggc agcgccgtca ccaccagctc tttgcccgga tgcaacagcg gaccataggt
    56341 cgcgttaacc tgttccaggt tcgccagcat attgcggtga gtcagcatcg cgcctttcgc
    56401 cacaccagtg gtgccgccgg tgtattgcag gaaagctaaa tcttccggca ccagttctgg
    56461 tttgacgtac tgcatccggt agccgttgtg cagggcgcta cgaaatgaaa tggcatctgg
    56521 cagatggtat ttcggcacca gacgcttgat gtatttaaca acgaaattga ctaccgtgcc
    56581 ttttgccgta gatagctgat cgcccatacg ggtcagaata acgtgctgaa cggcggtttt
    56641 atcaaccact ttttccagag tgtgggcaaa gttagacacg ataacaatcg ccgatgcgcc
    56701 gctatcgtta agctgatgct caagctcacg cggggtatac aacgggttaa cgtttacgac
    56761 gatcatcccg gcacgcaaaa tgccaaacag cgccaccgga tattgcaata agttaggcat
    56821 catcaacgca acgcgatcgc ctttcttcag ccccaaccct tgttgcaaat aagcggcaaa
    56881 cgcgcgactg cgttcttcca gtttgcggaa ggtcatcacc tcccccatat tcacaaacgc
    56941 gggttgatcg gcgtagcgcg cgaccgactg ctcaaacata tctaccagag attgataacg
    57001 gtcagggttg atctccgtcg gaacgtccgc gggataacgg ttaagccaaa ccttcttcaa
    57061 ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc ccaagtgata aacatactgt
    57121 taacataata ttaactcatc ttaccagctt gataattacc caaggaaaag gttgcgaagc
    57181 gcgtcactat ttatttttat ctttaccgta agaatgcaga aacagcggac cggccgctgt
    57241 ttctttttct ttaaaaacaa gcaattattc agttacaact gtttgtactc tcgcggggcc
    57301 gggattatac cagccccatc cgccgtagcc atagggccag ccacgaccgc cataaaacca
    57361 tggatcaatc ggctgaggcg gcataatcac ctgctgggtt aaatgccaac gtttgtaacc
    57421 cgttacttgc atcaccataa atttataggg cgtattaccg attttgccgt caaccgcacc
    57481 ggtgattggc ccgactaccg tcaccagttg tccgcggaaa tccaccgggt ccagaaaacc
    57541 gttcacatcg gcataaatgc gaccgcgaga aggttctccc agcgtcggtc tggctccgct
    57601 gtccagtggt acggtggcaa tttccaggcg tgttttccct tgctggtttt gtaccgcaac
    57661 cactttgccg ccaaagcgag cctcctgacc aacgtacagc tgcggcgcac tcatcacccg
    57721 aactaaatct tgttgcggcg tgggactgct gcctttaatg gcgtctggca cagtaacaca
    57781 accgctcagc attagcgcaa acgtacctgc cagtatgcct ttgataacat tcttttgaac
    57841 cgccatgatg cgactccttt ttttctcagg gcatactctt aagattcatt ctttgcccgg
    57901 aagtttcttc catgcgacgt tgttacgtaa ataaactggt tcagcatgtt ccaccgccac
    57961 cattttaccc tcggcaaaca tctggcacgc aatcggcagc atatcttctg cagcgggcag
    58021 taacacttcg ccatcgagca aaaccagccc gctctcttta ccgagatccg gccaggcttg
    58081 ccagcccgtc cctactgtca cccattcgcc ggaaagctgt tgcattcgtt catgaacgag
    58141 ttcgggtttg agtacggctt cggtttcttc accgtgccag ataccgtttt catcacgctg
    58201 atattcggcc cagtaaactt cgcccattcg cgcgtcaata gctgccagca cgcgggttgc
    58261 gccgtttttg cgccacgccc cttgcgccat cgtcattagc gtggagacgc caatcattgg
    58321 taactccgcg ccaagtgcca gtccttgcgc aatgccaata ccaatgcgca cgccggtgaa
    58381 gctaccgggg ccgcgcccgt aagccagagc gtttatgtca gtcagggaag ttccgctggt
    58441 ggtcaggata tcctgcacca tcggtaagat tcgttgagta tgttcacgag ggcaaagctc
    58501 aaaatgagcg ttgacagtac cgtcgttcca cagggcgaca gagcaggcct ctgtcgcggt
    58561 atcgatagcc agaattcgca tgggtcttcg tgcttagatc aataaaaagg cgcgcatcat
    58621 accatactcc gtaacaaatt acctggagga tggtatcgca aggaaacgaa ccgcacgggc
    58681 aatgtcacgg gtgcgcggcg ctggcggcag actggcgaga aacgtcgcgc cgtaagggcg
    58741 catcaccagc cgattgtcgc aaatcactaa cacgccgcga tcgtcggcgt cgcgaatcaa
    58801 tcgccctacc ccctgcttga gagtaatgac cgcatccggc agttgtactt catcgaacgg
    58861 atcaccaccg cgcaaacggc aatcttccat gcgtgctttt aatagtggat catccggcga
    58921 ggtaaacggc aatttgtcga taatcaccaa tgacaatgta tcgccacgca cgtccacccc
    58981 ttcccagaaa ctgctggttg ccacaagaag cgcgttaccg gcgctgacaa attgctgcaa
    59041 cagttgccct ttgctggttt ccccctgcaa caaaacggga agcgtcatgg tagcgcggaa
    59101 ctgctcggcc agatcgcgca tcatggcgtg cgaggtacaa agcataaaac aacgaccgtt
    59161 gttagcttcg atgatcggtc gcagcattgc cgccagttgc cgtgcggaac ccggttgatt
    59221 agtttgcggc aggttgcgcg gcacacagag taacgcctgg cggctgtaat caaaggggct
    59281 gggtaacagc aacgactctg ccagttcaat gccaagccgc gaggtgaaat gatgcagatc
    59341 gtcgttcacc gacagcgttg ctgaggtaaa gatccagcta ccgggttttt gcgccattaa
    59401 ctctttgaat ttatccgcca cgctgagcgg cgtgagagcc agagtaaaat ggcgcgaagt
    59461 gcattcgtac cagtagctgt agcccggctg attgatctct tttagccgtt tcagccgtgt
    59521 gcgatataac gtggcgcgtt caaatgccgc atccagcaag gcggaacgcc ccagcgacag
    59581 tttcgccacg tcataacaaa gttccagagt gtcatcgagc agtaaaaatg cccgctgaat
    59641 ttgcggatta gctaacagct cacgcaggtt accgcgataa cctggctcac cgagttgcag
    59701 gcgaaaatcc tgcgcactct gggcaagacg gtcagcgcac ttttgtaact gctgggtgtc
    59761 ttttaattcg gtgcggtagg cgatggtgat gtcttttgcc aggtcgagca gttgtcgact
    59821 ggagagtgac tgaccaaaat actggctggc aatgtcgggc agctggtggg cttcgtcgaa
    59881 gatcatgacg tcagcttccg ggatcagttc gccaaatcca ctctctttca ccaccatatc
    59941 cgccagaaag agatgatggt ttaccaccac cacatcggcg tccatcgctt ttttgcgtgc
    60001 tttgactaca aagcaatctt tatacatcgg gcagtcgctg ccaaggcagt tatcgttggt
    60061 gctggtgacc agcggccacg cctgtgaatc ttccgccacg ctgacgcagg tgctgatatc
    60121 accatcgact gtttgattag accaggagcg cagcaggatc acatcgctta atatttgtac
    60181 cggcagatcg ccgcccgcta gcgcctgttg ttcgagacgt tcgaggcaga ggtagtttga
    60241 gcgccctttc agcaacgcca cgttgcccgt atatttcaac gcctttgaaa ccgttggcaa
    60301 atcgcggctg tagagctgat cctgcaacgc ttttgaaccg gtcgagataa tgactttctt
    60361 tttcgcccgc agcgcagggg ccaggtaagc gtaggttttg cccgtaccgg ttccggcttc
    60421 caccaccagc ggctggcctt tttctatcgc ctgggtgacg gctaccgcca tctgtcgctg
    60481 tggttctcgc ggcttaaagc ctggtatcgc tttcgccaac tgaccgtctg gtgcaaaatc
    60541 gtccgtcaca ctaccccctg ttgatttgaa cagggattat gtcaggatga gggcgctttc
    60601 gccagttgaa gtggtgacgg cgacctcaca ttgtggcagt ctttgcagca cgaaaatgga
    60661 aggataatga ggacaaaatg atgactatcg ttcgtatcga tgctgaagcc cgctggtctg
    60721 atgtggtgat ccacaacaac acgctctact acactggtgt accggaaaac ctcgacgccg
    60781 atgcttttga ccaaaccgcc aacacgctgg cgcagattga cgccgtgctg gaaaaacagg
    60841 gcagcaataa atcgagcatt ctggatgcca ccattttcct ggctgataaa aacgacttcg
    60901 cggcgatgaa taaagcgtgg gatgcctggg ttgtcgcggg tcatgcgccg gtgcgctgca
    60961 cggtacaagc gggtttgatg aacccgaagt ataaagttga aattaagatt gtggctgcgg
    61021 tgtaagcttt atcgaagcaa aataagtcag acgataattt atcgataata ctggtcggtt
    61081 ttacataaat cgaccagaga ttaagatatt actgttgatg ctcctgctaa ttcattattt
    61141 attttt
//
LOCUS       DADTFG010000008        58129 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.008, whole genome shotgun sequence.
ACCESSION   DADTFG010000008 DADTFG010000000
VERSION     DADTFG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.008"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            360..1703
                     /gene="argG"
                     /locus_tag="J1138_000532"
     CDS             360..1703
                     /gene="argG"
                     /locus_tag="J1138_000532"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBA8452376.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(1711..3336)
                     /locus_tag="J1138_000533"
     CDS             complement(1711..3336)
                     /locus_tag="J1138_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBA8452377.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLIAVPFVLLIAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFFAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(3793..3879)
                     /locus_tag="J1138_000534"
     tRNA            complement(3793..3879)
                     /locus_tag="J1138_000534"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3843..3845),aa:Leu,seq:gag)
     gene            complement(3894..4226)
                     /gene="secG"
                     /locus_tag="J1138_000535"
     CDS             complement(3894..4226)
                     /gene="secG"
                     /locus_tag="J1138_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBA8452378.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4454..5791)
                     /gene="glmM"
                     /locus_tag="J1138_000536"
     CDS             complement(4454..5791)
                     /gene="glmM"
                     /locus_tag="J1138_000536"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBA8452379.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5784..6632)
                     /gene="folP"
                     /locus_tag="J1138_000537"
     CDS             complement(5784..6632)
                     /gene="folP"
                     /locus_tag="J1138_000537"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBA8452380.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6722..8656)
                     /gene="ftsH"
                     /locus_tag="J1138_000538"
     CDS             complement(6722..8656)
                     /gene="ftsH"
                     /locus_tag="J1138_000538"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBA8452381.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8756..9385)
                     /gene="rlmE"
                     /locus_tag="J1138_000539"
     CDS             complement(8756..9385)
                     /gene="rlmE"
                     /locus_tag="J1138_000539"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBA8452382.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9511..9804
                     /gene="yhbY"
                     /locus_tag="J1138_000540"
     CDS             9511..9804
                     /gene="yhbY"
                     /locus_tag="J1138_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBA8452383.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9960..10436)
                     /gene="greA"
                     /locus_tag="J1138_000541"
     CDS             complement(9960..10436)
                     /gene="greA"
                     /locus_tag="J1138_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBA8452384.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10684..12117
                     /gene="dacB"
                     /locus_tag="J1138_000542"
     CDS             10684..12117
                     /gene="dacB"
                     /locus_tag="J1138_000542"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBA8452385.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKRGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12158..13330)
                     /gene="cgtA"
                     /locus_tag="J1138_000543"
     CDS             complement(12158..13330)
                     /gene="cgtA"
                     /locus_tag="J1138_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBA8452386.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTEPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13346..14311)
                     /locus_tag="J1138_000544"
     CDS             complement(13346..14311)
                     /locus_tag="J1138_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA8452387.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14438..14695)
                     /gene="rpmA"
                     /locus_tag="J1138_000545"
     CDS             complement(14438..14695)
                     /gene="rpmA"
                     /locus_tag="J1138_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBA8452388.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14716..15027)
                     /gene="rplU"
                     /locus_tag="J1138_000546"
     CDS             complement(14716..15027)
                     /gene="rplU"
                     /locus_tag="J1138_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBA8452389.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15286..16257
                     /gene="ispB"
                     /locus_tag="J1138_000547"
     CDS             15286..16257
                     /gene="ispB"
                     /locus_tag="J1138_000547"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBA8452390.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16485..16763
                     /gene="sfsB"
                     /locus_tag="J1138_000548"
     CDS             16485..16763
                     /gene="sfsB"
                     /locus_tag="J1138_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBA8452391.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16811..18070)
                     /gene="murA"
                     /locus_tag="J1138_000549"
     CDS             complement(16811..18070)
                     /gene="murA"
                     /locus_tag="J1138_000549"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBA8452392.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18125..18379)
                     /gene="ibaG"
                     /locus_tag="J1138_000550"
     CDS             complement(18125..18379)
                     /gene="ibaG"
                     /locus_tag="J1138_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBA8452393.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18539..18832)
                     /gene="mlaB"
                     /locus_tag="J1138_000551"
     CDS             complement(18539..18832)
                     /gene="mlaB"
                     /locus_tag="J1138_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBA8452394.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18832..19467)
                     /gene="mlaC"
                     /locus_tag="J1138_000552"
     CDS             complement(18832..19467)
                     /gene="mlaC"
                     /locus_tag="J1138_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBA8452395.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19486..20037)
                     /gene="mlaD"
                     /locus_tag="J1138_000553"
     CDS             complement(19486..20037)
                     /gene="mlaD"
                     /locus_tag="J1138_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBA8452396.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(20042..20824)
                     /gene="mlaE"
                     /locus_tag="J1138_000554"
     CDS             complement(20042..20824)
                     /gene="mlaE"
                     /locus_tag="J1138_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBA8452397.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20832..21641)
                     /gene="mlaF"
                     /locus_tag="J1138_000555"
     CDS             complement(20832..21641)
                     /gene="mlaF"
                     /locus_tag="J1138_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBA8452398.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21851..22828
                     /locus_tag="J1138_000556"
     CDS             21851..22828
                     /locus_tag="J1138_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBA8452399.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGIALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22842..23828
                     /gene="kdsD"
                     /locus_tag="J1138_000557"
     CDS             22842..23828
                     /gene="kdsD"
                     /locus_tag="J1138_000557"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBA8452400.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23849..24415
                     /gene="kdsC"
                     /locus_tag="J1138_000558"
     CDS             23849..24415
                     /gene="kdsC"
                     /locus_tag="J1138_000558"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBA8452401.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTHIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24412..24987
                     /gene="lptC"
                     /locus_tag="J1138_000559"
     CDS             24412..24987
                     /gene="lptC"
                     /locus_tag="J1138_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBA8452402.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24956..25513
                     /gene="lptA"
                     /locus_tag="J1138_000560"
     CDS             24956..25513
                     /gene="lptA"
                     /locus_tag="J1138_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBA8452403.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25520..26245
                     /gene="lptB"
                     /locus_tag="J1138_000561"
     CDS             25520..26245
                     /gene="lptB"
                     /locus_tag="J1138_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBA8452404.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26293..27726
                     /gene="rpoN"
                     /locus_tag="J1138_000562"
     CDS             26293..27726
                     /gene="rpoN"
                     /locus_tag="J1138_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBA8452405.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27749..28036
                     /gene="hpf"
                     /locus_tag="J1138_000563"
     CDS             27749..28036
                     /gene="hpf"
                     /locus_tag="J1138_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBA8452406.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28154..28645
                     /gene="ptsN"
                     /locus_tag="J1138_000564"
     CDS             28154..28645
                     /gene="ptsN"
                     /locus_tag="J1138_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBA8452407.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28691..29545
                     /gene="rapZ"
                     /locus_tag="J1138_000565"
     CDS             28691..29545
                     /gene="rapZ"
                     /locus_tag="J1138_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBA8452408.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29542..29814
                     /gene="npr"
                     /locus_tag="J1138_000566"
     CDS             29542..29814
                     /gene="npr"
                     /locus_tag="J1138_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBA8452409.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            30027..30659
                     /gene="yrbL"
                     /locus_tag="J1138_000567"
     CDS             30027..30659
                     /gene="yrbL"
                     /locus_tag="J1138_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBA8452410.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVTPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30656..31384)
                     /gene="mtgA"
                     /locus_tag="J1138_000568"
     CDS             complement(30656..31384)
                     /gene="mtgA"
                     /locus_tag="J1138_000568"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBA8452411.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     MAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31381..32034)
                     /gene="elbB"
                     /locus_tag="J1138_000569"
     CDS             complement(31381..32034)
                     /gene="elbB"
                     /locus_tag="J1138_000569"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBA8452412.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32264..34600)
                     /gene="arcB"
                     /locus_tag="J1138_000570"
     CDS             complement(32264..34600)
                     /gene="arcB"
                     /locus_tag="J1138_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBA8452413.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKAEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34696..35625)
                     /locus_tag="J1138_000571"
     CDS             complement(34696..35625)
                     /locus_tag="J1138_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBA8452414.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            36300..40760
                     /gene="gltB"
                     /locus_tag="J1138_000572"
     CDS             36300..40760
                     /gene="gltB"
                     /locus_tag="J1138_000572"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBA8452415.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40773..42191
                     /locus_tag="J1138_000573"
     CDS             40773..42191
                     /locus_tag="J1138_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA8452416.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42374..43501
                     /locus_tag="J1138_000574"
     CDS             42374..43501
                     /locus_tag="J1138_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HBA8452417.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(43561..44025)
                     /locus_tag="J1138_000575"
     CDS             complement(43561..44025)
                     /locus_tag="J1138_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA8452418.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARECEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPDEIKKVVVKVKADLMA"
     gene            complement(44022..44897)
                     /locus_tag="J1138_000576"
     CDS             complement(44022..44897)
                     /locus_tag="J1138_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBA8452419.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIATAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44894..45583)
                     /locus_tag="J1138_000577"
     CDS             complement(44894..45583)
                     /locus_tag="J1138_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBA8452420.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45631..47121)
                     /gene="nanT"
                     /locus_tag="J1138_000578"
     CDS             complement(45631..47121)
                     /gene="nanT"
                     /locus_tag="J1138_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBA8452421.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNNLVKTKS"
     gene            complement(47230..48123)
                     /gene="nanA"
                     /locus_tag="J1138_000579"
     CDS             complement(47230..48123)
                     /gene="nanA"
                     /locus_tag="J1138_000579"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBA8452422.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(48245..49036)
                     /gene="nanR"
                     /locus_tag="J1138_000580"
     CDS             complement(48245..49036)
                     /gene="nanR"
                     /locus_tag="J1138_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBA8452423.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(49144..49641)
                     /gene="sspB"
                     /locus_tag="J1138_000581"
     CDS             complement(49144..49641)
                     /gene="sspB"
                     /locus_tag="J1138_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBA8452424.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(49647..50285)
                     /gene="sspA"
                     /locus_tag="J1138_000582"
     CDS             complement(49647..50285)
                     /gene="sspA"
                     /locus_tag="J1138_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBA8452425.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(50680..51072)
                     /gene="rpsI"
                     /locus_tag="J1138_000583"
     CDS             complement(50680..51072)
                     /gene="rpsI"
                     /locus_tag="J1138_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBA8452426.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(51088..51516)
                     /gene="rplM"
                     /locus_tag="J1138_000584"
     CDS             complement(51088..51516)
                     /gene="rplM"
                     /locus_tag="J1138_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBA8452427.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(51736..52863)
                     /locus_tag="J1138_000585"
     CDS             complement(51736..52863)
                     /locus_tag="J1138_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBA8452428.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDENRTQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            53057..53455
                     /locus_tag="J1138_000586"
     CDS             53057..53455
                     /locus_tag="J1138_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBA8452429.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            53609..54976
                     /gene="degQ"
                     /locus_tag="J1138_000587"
     CDS             53609..54976
                     /gene="degQ"
                     /locus_tag="J1138_000587"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBA8452430.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESL
                     YLLMR"
     gene            55066..56133
                     /gene="degS"
                     /locus_tag="J1138_000588"
     CDS             55066..56133
                     /gene="degS"
                     /locus_tag="J1138_000588"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBA8452431.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(56196..57134)
                     /gene="mdh"
                     /locus_tag="J1138_000589"
     CDS             complement(56196..57134)
                     /gene="mdh"
                     /locus_tag="J1138_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBA8452432.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            57569..58039
                     /gene="argR"
                     /locus_tag="J1138_000590"
     CDS             57569..58039
                     /gene="argR"
                     /locus_tag="J1138_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBA8452433.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        14452 a        15592 c        14406 g        13679 t
ORIGIN      
        1 ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac
       61 tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccacg
      121 ccacattttg tcaacgttta ttgctaatca cgtgaatgaa tatccagttc actttcattt
      181 gttgaatact tttaccttct cctgctttcc cttaagcgca ttattttaca aaaaacacac
      241 taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa
      301 aattcagtga aagcagaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta
      361 tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg
      421 gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat
      481 atactgcaaa cctgggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca
      541 tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag
      601 gtattgccgc tattcagtgt ggcgcatttc ataacaccac tggtggactg acctatttca
      661 acacgacgcc gctgggccgc gccgtgaccg gcaccatgct ggttgctgct atgaaagaag
      721 atggcgtgaa tatctggggt gacggcagca cctataaagg aaacgatatc gaacgtttct
      781 accgttacgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg
      841 actttattga tgaactgggt ggccgtcatg agatgtctga atttatgatt gcctgcggtt
      901 tcgactacaa aatgtctgtc gaaaaagctt actccacgga ctccaacatg cttggtgcaa
      961 cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccaatta
     1021 tgggcgtgaa gttttgggat gagagcgtga aaatcccggc agaagaagtc acagtacgct
     1081 ttgagcaagg tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga
     1141 tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaaa
     1201 accgtatcat cgaagcaaaa agccgcggca tttatgaagc ccctgggatg gcactgctgc
     1261 acattgcgta tgaacgtttg ttgaccggta ttcacaacga agacaccatt gagcagtatc
     1321 acgcgcatgg tcgtcagttg ggccgtctgc tgtaccaggg acgttggttt gattcccagg
     1381 cgctgatgct gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta
     1441 ctctggagct gcgccgcggg aacgattatt caatcctgaa taccgtctca gagaacctga
     1501 cctacaaacc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc
     1561 gtatcggtca actgaccatg cgtaacctgg atatcaccga tacccgcgag aaacttttcg
     1621 gttatgccaa aactggcctg ctctcctcct ctgccgcttc aggggtgccg caggtggaga
     1681 atctggaaaa taaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc
     1741 ttgattatcc tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc
     1801 atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg tttgcggctg
     1861 ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa
     1921 gatgttgttt tccgttgtgc gatccacgcc atcccctaga acggggctat accagataaa
     1981 catcgggaca tgatacgcct gctggctagc ctccctgcct ccatgaaaat agacgttctt
     2041 cttcgtaggg tcgcgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc
     2101 ttttaataat tcaaaaacct gacctagcaa actatctgtg taatgaatgg agttgtcata
     2161 gcaggcatcc tgatcgtcct gcggttgaaa cacagcgctg gattgcggat aggcgctaca
     2221 agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct gcgtcttttg
     2281 ctgtaacgct tgcgataaat gcggcaacaa caattcatca aatcctctga catagactgt
     2341 ttccatggcg cgcatggcga tactggtaac tgctgtacca ttctgccgaa aagcggattg
     2401 cgagcttagc cagaaagtct gaaatcctgc ttgattagcc atattaataa tgttgtccgg
     2461 gtaattatga atgtcatgac tcaaaacaga atcagcagtt aaagaaaggg gaaccgacag
     2521 cgcagtgtaa ggtgcaccgc ttattgcttg attaaacagt ttgatctgct ttctttgtgc
     2581 ttcgacttgc ggtgtcgtag agcgtgtata tccatacaaa gacatattgt cgacacgtac
     2641 agactccccc acaatcaaca cgtaggtatc aatacctgta tccctgactg atagttgaaa
     2701 atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc tctttcgctg ctaaagcaaa
     2761 ataattgaga ttgaaaaacg gcgtataggt agcaaatcgc gacgctagta tatatggact
     2821 gaacgcctct ttattttttg catctttata agcaaattga caagcggaaa ataaactgcc
     2881 cgaaatgaca atcagcaata atattcctgt cacttttttt gtcggcaagg aaacatcata
     2941 ttttattatt actgcaaaaa aaagaaggga taaaaaggca aaagcacata gataagggat
     3001 atacattccc agcattttga caacttcatc cggatcactc tgcagcacac taatcgcaaa
     3061 tccatcattg aaagttgtac caaagataca ccatgaatag aggctaatac tcatatctgc
     3121 cgcaatcagt aacacaaatg gtacggcaat aagcaggcga agcggaaggg atgatatacg
     3181 tttaacaacc aggatggtca gaacgaagaa cagcaatcgc tgaaccatat gcgctccgga
     3241 agaagcgact aagttcgtaa taccaaaaac aataacacaa agatacaaca accaatgaga
     3301 tttaaaactt ttaacaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
     3361 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgatcca
     3421 tatcagaccg aaacactgtt agattttttc tttttaaatg atgcggccat tgcgttctca
     3481 gtaattaaat aacattatca atgcgtctct aaacattacg cagaacgtag ccatcagtag
     3541 ttatactgac aggggcagga tggtttatcg aggagtaaac atatccgtat tcgtaccgga
     3601 aatggagttg acacaataaa gtgccaatta tgtcagcaga agggaaaagc ttataactaa
     3661 ggcataccgg caccatcgtt tcaaggtacc agctacgagt aaaacaactg gacgagatac
     3721 agatacctga caaccattcc tcagaccagg accaaaacga aaaaagacgc ttttcagcgt
     3781 ctttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
     3841 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
     3901 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggc
     3961 gcactcaggt tttcccattc gctaccttta ttggttttgt tgctattgat gttacccagc
     4021 accagactga tgatgaagaa taacgttgcc agcagcgctg tcatgcgggt catgaagtta
     4081 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
     4141 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
     4201 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
     4261 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
     4321 taaccaaagc gaatgacctt cgcaagggca attttattgc attgtatcaa ctgcggaaaa
     4381 aaacagcaaa accgatgtgt tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca
     4441 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
     4501 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
     4561 gcaacagcac acgtccacgg ctacccagcg ccgcttcaac ttctgcggtc acagctttga
     4621 ctgattcatg ctccagcgga tcgccgctac ctgcggtgta acgcacgttt accaggattt
     4681 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatgatta cgcgccatcg
     4741 ccgccagcac ctgcaaacca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
     4801 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
     4861 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
     4921 caagccccat gttgctcatc aatgtaccca cagcgccacc acgcagctgg ccctgacgaa
     4981 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
     5041 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
     5101 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
     5161 caccgttcgg ctcacaaccg atagcgataa cgttcgctcc cagttcacgc agcacgttcg
     5221 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
     5281 taagttcgtt tgggaacgtg gctttgcaaa actcgatata gcgacccgca gcatcaacga
     5341 tacggctggc tttacccagt tctgccgaat caacacagct gatctctttt tccatttccg
     5401 cttcgatggc ctcttctact gcatccggta gtttggtgcc gtcgatagag aaaaatttaa
     5461 tgccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
     5521 aggtacgcgt cagataagcc acggccggtg ttggcatcgg accagtgaag agtgcggaaa
     5581 ggcccgcagc cgccagaccc gcttccagtg ctgactccag catgtagcca gaaatacgcg
     5641 tgtctttacc aatgatgatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
     5701 aacccagctt aagcacaaaa tcaggggtga tcggcgcatc ccctacacga ccacgaatcc
     5761 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
     5821 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatatgc
     5881 gcgccttgca tagcggcaat gaccgcacaa gccagactac cgctcagacg ctcggacggc
     5941 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
     6001 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
     6061 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
     6121 cgtgctattt gctcaataaa atagcgattc acttctgcaa agacatcgtc atacttcggg
     6181 gcttcctgca tggtctttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
     6241 tctgcagctg cctccagagc ccctggttcg gaaagggagc ggatatcgtt aataatgtga
     6301 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
     6361 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
     6421 acgctaactt ccgccgctcc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
     6481 ccagcgttga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtaccg
     6541 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
     6601 tccagtgaag tgccctgggc aaagagtttc atggtgttat ccctggtatg aattgataag
     6661 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
     6721 cttatttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
     6781 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
     6841 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
     6901 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
     6961 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
     7021 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
     7081 acacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
     7141 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
     7201 aaacatgttc cggcccgtag atgatctctt ccgccagacg accaccgtac agcgtagaaa
     7261 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
     7321 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga tcgtgttccg
     7381 gcaccaggcg accgataatt gcgtgacccg cttcgtggta agccgtcgat tctttctgcg
     7441 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
     7501 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
     7561 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
     7621 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac cttcaggatc tgctcacgac
     7681 cgcgaacatc tggcaagcca accacaacct gacggtcgaa acggccagga cgcagcagcg
     7741 ctgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
     7801 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
     7861 cgcccagacc tgcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
     7921 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
     7981 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
     8041 caatcgcttt cgccagcagc gttttaccgg tacccggagg accgaccatc aggacgcctt
     8101 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tcaaccagtt
     8161 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
     8221 gatcttcagt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
     8281 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacaac atcgggaacc
     8341 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
     8401 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
     8461 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
     8521 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
     8581 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
     8641 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
     8701 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
     8761 gtttacgccc ggtcgctaca atatacactt cccgcgaacg tgcacgagaa gagtccggct
     8821 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
     8881 cgccttggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
     8941 ctagcgccag ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca
     9001 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
     9061 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
     9121 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
     9181 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
     9241 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaacccct
     9301 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
     9361 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
     9421 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
     9481 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
     9541 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
     9601 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
     9661 atcgccaccg aagatcgcga aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
     9721 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
     9781 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacattcg ctgtgtaaaa
     9841 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
     9901 gcatagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
     9961 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    10021 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    10081 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    10141 gaatcgagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    10201 ttggtgacat caatcacctg cgcgttcgac agcttggctt cgatgtcttt gatacggcct
    10261 tcgcaaaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    10321 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    10381 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    10441 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    10501 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    10561 ggtcagctac aattcacatt ttgatagtca ttttaccctg aagttcccga agggtcatcg
    10621 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    10681 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    10741 gccgcaaatg ttgatgagta cattactcaa ctccccgctg gtgccaacct tgccctgatg
    10801 gtgcaaaaag tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    10861 cccgccagta cgcagaaagt gattactgca ctggcggcgt tgattcaact cggccccgat
    10921 tttcgtttta ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    10981 gacttagtgg cgcgatttgg tgccgatccg acgttaagac gtcaggatat tcgcaatatg
    11041 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    11101 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    11161 tttagcgctc cgcctgccgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    11221 agtgccccaa aacgtggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    11281 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgagctg
    11341 gatgtggtgc caggcgacct gaaccgcttt acgctgaccg gatgcctgcc acaacgttct
    11401 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    11461 aaagatgagt taaaacaggc aggtatcacc tggagcggaa cactgctgcg ccagactcag
    11521 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    11581 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgataggc
    11641 catgcgcgct tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt gcgtcagatc
    11701 ctgcgccagc aagccggtgt cgatattgga aacaccatta ttgccgatgg ttcagggctt
    11761 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    11821 cacgacaatg aacttaactt tatctccatg ctgccgctgg cgggctatga cggctctttg
    11881 cagtaccgtg caggtctgca tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt
    11941 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    12001 gcgtttgtgc aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt
    12061 attccgttgg tacgttttga aagccgtttg tataaagata tttaccagaa caattagtct
    12121 aaagagaaac cccggcacat ggccggggtt ttgctgatta acgcttgtaa atgaactcaa
    12181 cgccttcttc gtcgtcttcg tcccagtcgt catcccagtc ttcatcatcc tcttcagcaa
    12241 tctcttcaag ctgctggcga tgataatcat cccacatgaa ttcgactttc tctggctgtt
    12301 tcgcttcttc agcctgcacg accgggtttt caatgataaa ggtcatcaca tcccagcaga
    12361 gatctttcac gcccagtccg ctcgccgcag agatcagata atatttatct tcccaaccca
    12421 gcgcctcagc gatcgctttc gctttctctt cggcttctgc cttatccagc aggtcgatct
    12481 tgttgaagac taaccaacgc ggtttcgccg ccagatcctg gctgtatttt tccagctcgc
    12541 tgataataat acgcgcgttt tcaaccggtt cggtgccgtc aatcggatcg atatcgatga
    12601 ggtgcaacag aacgcggcaa cgttccaggt gcttcaggaa gcgaatgccc agacctgcgc
    12661 cttctgcagc gccttcaatc agtcctggaa tatcggcaac aacgaagctc ttttcgttgt
    12721 ccattcgtac cacaccaaga cttggcacca gagtggtaaa cggataatcc gccactttcg
    12781 gtttagccgc cgataccgca cgaataaagg tcgatttacc cgcgtttggc atccccaaca
    12841 taccgacgtc agccagcagc atcagctcca gcagcagctc acgcttatcg cccggcgtac
    12901 cgttagtttt ctgccgtggt gtacggttaa ctgacgattt gaaacgggta ttgcccagac
    12961 cgtgccagcc gcccttagca accagcagac gctgaccgtg tttggtcata tcacccatgg
    13021 tttcaccagt accctggtcg attacacgcg tacctaccgg cactttaatc gtgacgtctt
    13081 taccgcgctt accggtacag tcgcggcttg caccgttttg accgcgctct gcacggaaag
    13141 atttttcaaa acgataatcg ataagcgtgt tcaggttctc gtcggcttcc atccatacgt
    13201 caccaccatc accgccgtcg ccgccatccg ggccgccttt cggaatatac ttttcgcggc
    13261 ggaagctcac gcaaccatta ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa
    13321 caaacttcat tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg
    13381 tatgcttacg taatccgccc caaattcgat gaccaatggc ggaatacatc gcgcccgcaa
    13441 ccacgacaaa cgcaccgaga taacctaaaa ggtttaacat cggtctggcg aagaaatcgg
    13501 gccaggccag tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg agcgtgatga
    13561 tcgcgctcac ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg ccatatccta
    13621 ccaatgtatt cagtccgcaa aaaattaaac atgcgagctg ccagtggcta agctgcgcta
    13681 tcactccagg atttgccaga gggaagagcg caattgtaca taaagtgtac agcaaaaaca
    13741 ggatctgcgg tgaggccagc cgacgcaata aaaccttttg cgccacgcca taactcaccc
    13801 aaaccgtcgc cgcaccgacc ccaaagataa ctccccaggt gtaatcggtg agctttgtaa
    13861 atatctcgac cagactggtg ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc
    13921 ctacaacctg agtactgcgc attttctctt tcaggataaa tacgctggca accatcatgc
    13981 caactggtga gagttgccca atcacctgcg aagcggtcgg actcaggtat tgcaaggatg
    14041 agctgaacag gatgaagttc ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc
    14101 agcgtggctt acgaaacacg cgtaatggcg gcaaccgctt cttcaccgca agaatggcac
    14161 caaggccaat actcgccatc aagaaacggt aaaacacgat tgtcggaggt tccatcacct
    14221 ccagcacctg cttcattgcg attggcaacg ccccccagca aattgctgtg gtgagcgcca
    14281 aaagaatgcc aatgcctgcc tgctgcttca taccgtattc cctgcaaagc gcatttgcgg
    14341 attaacgtcg caccatgcgc atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg
    14401 ttgcggggct ttcatccgtt accgggacgc gaaaaactta ttcagcttcg atgctgataa
    14461 atttacggtt tttcgggcct ttaacttcga atttcacttt accgtctgct ttagcaaaca
    14521 gagtgtggtc acgaccgcaa cctacgttag cgccagcgtg gaatttggta ccacgttgac
    14581 gaacgatgat gctacccgcc agaacggatt cgccaccgaa acgcttaacg cccaggcgtt
    14641 tagcttctga atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt gccatttgaa
    14701 atctctcctc aggtcttagg cgctgatgcc agtaattttc acatcagtga accactgacg
    14761 atggccctgc tgcttacgat agtgtttacg acgacgaaac ttaacgattt taactttctc
    14821 gccacgaccg tgagcaacaa cttcagcttt gattacgccg ccatcaacga aaggaacgcc
    14881 gattttgact tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc
    14941 agttgcgatg tccagctttt ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt
    15001 accaccactt tggaaaaccg cgtacatata aaactccgct tccgcgcaca ccttttcaat
    15061 gattcagagt gcgctataaa tattcacaat agggcgcgaa tattacgcaa aacgcacgcc
    15121 tttgacaagt gctacagtca atacacgaag aaaaaaaaca caacttgtac ggtaacgttt
    15181 atctgtgcca ttttttcagt acaatcaccc tatattccta accataaacc ctaagttgcc
    15241 tttgttcaca gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa
    15301 atcaatgagt taaccgcgca agatatggcg ggtgttaatg cggcaatcct tgagcagctt
    15361 aattccgacg tccaactgat caatcagtta ggctattaca tcgtcagcgg cggcggtaaa
    15421 cgtattcgtc cgatgattgc tgtactggct gcacgagctg ttggctatga gggaaatgcg
    15481 catgtcacca ttgctgccct gatcgagttt atccacacgg cgactctgct gcacgacgac
    15541 gttgtggatg aatcagatat gcgcaggggt aaagctaccg ccaacgccgc atttggcaat
    15601 gccgccagcg tgctggtagg cgattttatt tatacccgcg cgttccagat gatgaccagc
    15661 ctcggctcgc tcaaagtgct ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt
    15721 gaagttctgc aactgatgaa cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc
    15781 gttatctata gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc cgggattctg
    15841 gctggctgta cgccggagga ggagaaaggc ctgcaggatt atgggcgcta tctcggcact
    15901 gctttccagt tgatcgacga tttactcgat tacaatgccg atggcgaaca gttaggtaaa
    15961 aacgtcggcg acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca tgcaatgcat
    16021 catggcacac cagaacaggc acagatgatt cgtaccgcca tcgaacaggg taacggtcgc
    16081 catcttctgg aaccggttct ggaagcaatg aacgcttgtg gatctcttga atggacgcgt
    16141 cagcgtgccg aggaagaagc agacaaagcc atcgcagcgt tacaggtgct cccggacacc
    16201 ccttggcgag aagcactcat cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc
    16261 cctcccctca tcccgcgcag cgcgcgggat gattccagta aattccataa aaacacttat
    16321 tcagctctat aacacctgaa aaatagggct gtacatcttt atatattctg aatattcaca
    16381 ctctttacag gaacttttta gagcaatggg ccatcaggag tatagtgatg ctcgacagaa
    16441 gaagtgttct gaatgaaagc gactaactta aggagtgagg aaagatggaa agtaatttca
    16501 ttgactggca tcccgctgac atcattgcgg gtttgcgcaa gaagggaacg tcaatggcgg
    16561 cggaatctcg cagaaatggt ttgagttcct caacgctggc gaacgcatta tcgcgcccct
    16621 ggccgaaagg agagatgatt attgcgaaag ccctgggaac tgacccctgg attatctggc
    16681 catctcgcta ccatgatccg cagacccatg agtttatcga cagaacgcag ttgatgcgta
    16741 gctacactaa accgaaaaaa tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc
    16801 tctcagacga ttactcgcct ttcacacgct caatatttgc acctaaagcg cgcagtttgt
    16861 cttcaatgcg ttcgtagcca cgatcgatgt gataaatacg atcaaccacg gtcgtccctt
    16921 ccgcaataca gccagccaac accaggcttg ctgatgcacg cagatcggtt gccataacct
    16981 gtgcgccaga aagtttttca acaccgtgac aaataacggt attgctttcg atttcggcgt
    17041 gcgcgcccat acggctcagc tctggcacat gcataaagcg gttttcaaag accgtttcgg
    17101 tgatgaaccc ggtcccttct gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg
    17161 tcgggaatgc cggatgcggc gcggtacgta cgttaacagc cttcggacgt ttgccatgca
    17221 tatccaggct aatccagtct tcgccgactt cgatgtccgc tccagcgtca cgcagtttcg
    17281 ccagcacggc gtccagagta tctggctgcg cgttacggca gataattttg ccgcgagaga
    17341 tcgccgccgc caccaggaaa gtaccggttt cgatacgatc cggcagcacg cgatagacac
    17401 cgccgcctaa acgttccaca ccttcaatga cgatacgatc ggtgccctga ccgctaattt
    17461 tcgcacccag cgtaatcagg aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt
    17521 tttcaataat cgtggtgcct tcggccaggg ttgcagcaca catgatggtc accgttgcgc
    17581 caacgctgac tttatccatc acgatatgtg cgcctttcaa acgaccatcg acggaagctt
    17641 taacgtaacc ttcttccagt ttgatggtcg cgcctaattg ttcgagacca gaaatgtgta
    17701 gatcaaccgg acgtgcacca atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa
    17761 agcgcgctac cagcggcccc agcgcccaga tagaagcacg catggtttta accagatcgt
    17821 aaggtgcgca gaatacatta acgtcgcggg catcaatgtg cacagaacca ttacgttcta
    17881 ctttcgcacc cagctggctt agcagcttca ttgatgtatc gacgtctttt agtttcggga
    17941 cgttctggat ctctaccggt tcttccgcca gtagtgcggc aaaaaggata ggcagagcag
    18001 catttttagc gccggaaatt gtgacttcgc cctggagctt cgttggcccc tgaacacgaa
    18061 atttatccat ttagtttgtt ctcagttaac aattcatatc cgctaccggc gaatcgccca
    18121 tagctcaaaa gccgttcagt ttgcgatcgc gcgcccactc cgcaggggta tacgctttga
    18181 tcgacacagc atgaatgcgg ttatccgcaa tatattccat cagcgggcca tagaccgtct
    18241 gctgtttttt aacccgactc atgccgtcaa acaactcacc cacggcaata acctgaaagt
    18301 ggctgccatc gccggaaacg tggacttcct ggagggagag agcgttcatc aacacgctct
    18361 gaatttcatt attttccatg ggatcttcaa tcatcagtta ataaatcagc gaaacatctt
    18421 agagcaaagt tgcgctggca taaataagca aaaagcctcg ctgataaatc agacaaggct
    18481 cgacttgcag gcaggtttgc cggacaggcg gttaacgcca tatccggcct gaaaaaattt
    18541 aacgaggcag aacatcagca ggcaaattat acaatttcgc cagggtatac actttgtcgt
    18601 ttaccccctg aagcgtcaca ttgttgccct gctttttcgc cagatcgata agatggagca
    18661 gcagtgccag tccacccgta tccacgcgag acacacggct aagatcgatg ctggtaattc
    18721 ccttcaccgc ttcctcacgt atttcccaaa gcggtagcag aacgtcctga tccagctctc
    18781 cggataacgc cagcgtgtca cccgtctgca tccagctcag tgactcgctc attatttttt
    18841 ctcttccaga gtgattttct gttgagaaat cgatttcagt tgcgcagtca ggccgtcgat
    18901 acctttggta cgcagcagcg ttccccactc gttttgtttg gtggtgatca tactgacgcc
    18961 ttcagcaatc atgtcgtaag cctgccagtt gcccgtctgg gagtttttac gccactggaa
    19021 gtccagacgc accggcgggc ggccattcgg gtcaataatg gtaacgcgaa tcggcacaat
    19081 ggttttatca cccagcggct gttctggcgc aatctgatag gtttgaccgt gatacatcgc
    19141 cagcgcctga ccgtaagcct gcttcaggta ctcacggaaa gcggcaaagt aagcatcacg
    19201 ttgagctggg gtcgcactct tgtaatactg gcccagcacc agcgcaccgg cgtatttcac
    19261 ctgtacgtat ggcaacagtt cctgatcaac aatggtacgc agataatccg ggttggcccg
    19321 aatttgcggt tgctcattct tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag
    19381 cttatacgga ttggtctggt ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa
    19441 agcgaccatc attaaacgtt taaacatgcg tcggttctcc tgaaattatt tcgttgtacc
    19501 cacaggttca gtggtttcat tattacctgg cgcagcagct ggcgcatcgc cactattctt
    19561 attgtcatcg cctttactac cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc
    19621 agacttggtg tcctgaattg tatcgccatc cttcaggata gcagtcccca gttccgggtc
    19681 ttcaaaaccg acgtttaatg ccagatattg ttcccccagc aggccggaag tacgaatgct
    19741 cagcgaactg gtatcaggaa tgtggttata acgttgttca atttccagcg ttacgcgcgg
    19801 cagataggtt ttcgggtcca gcgtaatatc cgccacccgg cccacaacaa cgccaccaat
    19861 gctgaccgga gagcgggctt tcaggccgcc aatgttatcg aacgtcgcat aaagcgtata
    19921 ggtcggttca gtacgtatag acgtcacgtt cgctgccttc aggcaaacaa acagcgccgc
    19981 aagcagtgct gctaataaaa agatacccac ccaaatttca ttttttttcg tttgcatgaa
    20041 ctcaattccc aaacatcaat gcggtcagca caaaatccag ccccagaaca gccagagacg
    20101 agtggacaac ggtgcgagtg gttgcccggc taatcccggc agacgtcggg atggcgtcgt
    20161 agccgttaaa caacgaaatc cacgtcaccg tgatggcgaa caccacgctc ttaatcagac
    20221 agttgacgag atccatacgc cagtcgacgg cattttgcat tgccgaccag aagaacccac
    20281 tatcaatgcc tttccaactg acgccgacca gcgatccgcc ccagatcccc acggcgacga
    20341 agataaccgt caacagtggt aatgaaataa ctccagccca gaaacgggga gaaataaccc
    20401 gacgcagcgg atccacagcc atcatctcca tactggagag ttgctctgta gcgcgcatca
    20461 ggccgatttc tgcggttagc gccgaaccag cacgcccggc aaacaacaac gcggcaacca
    20521 ccggccccag ttcacgcagt agcgataacg ccaccagcat acccagactg gtttccgcac
    20581 tataagtggt cagaaccaga taaccttgca gccccaacac cattccgatg aacacgccag
    20641 aaaccacaat aatcagcatc gacaggacgc cgacattata gagctggcgc accagcagcg
    20701 gtgcatgttt gcgaaattcc ggtttgccga ccagcgcatt gaataacatt aacccggccc
    20761 gcccgaacgt tctcagggtt ttaatccctt tatgtccgag cgacgccagc gcatttaaca
    20821 gcatgagtgg cttaactccc tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg
    20881 aacggaacag gcccgtcagc tatcccgtcc agaaactgac gtacgcgcgg atcaggattc
    20941 gcctgcaacg cctgggcact gccatgagcg acaatttttt tgtccgccag gatccaagcg
    21001 tgatccgcaa tacttaacac ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc
    21061 agcgcgctgt tcagctcaga aatcagcttc accagcacgc ccatggtaat gggatcttgc
    21121 ccaacaaagg gctcatcaaa catgatgaga tccggctcca gcgcaatcgc acgtgccagc
    21181 gctgcacgcc gcgccatccc accggaaagt tcagaaggca taagtttagc cgctccacgc
    21241 agccccacgg cctccagctt catcatcacc gtactatgca acaatggcgc gggaagttgg
    21301 gtatgttccc gcagtggata ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc
    21361 ccggactgaa ataacatgct catccgtttg cgcactgtat acaggcgcga acgagacatc
    21421 gccggaatat tctcaccatc gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca
    21481 atcagacgga gtagcgtcgt tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc
    21541 cctcgcggca cggtcaggga aatattatcg aagatgcagc gattgccacg cgtaaaactg
    21601 acatcgcgca tatcgactaa attcgccaca gactgctcca taattcaccc ttcgtcttgc
    21661 gttgattttc taagcatggc gctcaattta accttgaacc caacatattt acagaatatt
    21721 acctgccgtg gttagcgaaa gctggcattt gttttacttt ttagccgcat aaagtcaaaa
    21781 ttaagaatcc gttacggctt tctgaaaatc ttcagcggac cggcgagtat acctgaagaa
    21841 aggacgttag atgcttttag ctacggcact gttaattgtt ggtttacttt tggtcgttta
    21901 cagtgccgac cgcctggttt ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc
    21961 gctgatcatc ggcatgacgg tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc
    22021 gcttgccgcg tcactgcacg aacaacgtga tttagccgtt ggtatagccc tcggctcaaa
    22081 cattatcaat atattgctga tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca
    22141 ttctgatgtt ctacgccgtg aattaccctt aatgttgttg gtcagcgtgg tggccggttc
    22201 cgtactctat gacggacaac ttagtcgcag cgatggtatc tttctcctct ttctggctgt
    22261 gctatggctg ctgttcattg ttaaacttgc acgtcaggct gaacgtcagg ggactgacag
    22321 cctgaccaga gagcagcttg cagagctgcc gcgtgagggc ggattgcccg tcgcattttt
    22381 atggctcggc atcgcactga tcattatgcc agtggccacg cggatggtag ttgataacgc
    22441 cacggtgctg gcgaattact ttgccattag cgagttgacg atgggtctga cggcaattgc
    22501 tatcggaacc agcctgccgg aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa
    22561 cgacattgcg gtcggaaata tcattggcgc aaacattttt aatattgtca tcgtgttggg
    22621 tttacccgcg ctgataacgc caggagagat tgatccactg gcgtacagtc gtgactacag
    22681 cgtgatgttg ctggtgagca ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc
    22741 gggccgtggt gtaggggtat tattaactgg cggatttatc gtatggctgg cgatgttgta
    22801 ctggttatcg ccaatactcg ttgaataact ggaaacgcat tatgtcgcac gtagagttac
    22861 aaccgggttt tgactttcag caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc
    22921 tggcggagct tgatcaatac atcaatcaga atttcacgct tgcctgtgaa aagatgttct
    22981 ggtgtaaagg gaaagttgtc gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa
    23041 tggcagcaac atttgccagc accggtacac cttcattttt cgtccatcct ggcgaagccg
    23101 cgcatggtga tttaggcatg gtcaccccac aggatgtggt gattgctatc tctaactcag
    23161 gtgaatccag cgaaatcacg gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa
    23221 tctgcatcac cggtcgcccg gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg
    23281 ttaaagtggc gaaagaagcc tgtccgttag ggctggcacc gaccagcagc acaaccgcca
    23341 cgctggttat gggcgatgcc ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg
    23401 aagattttgc gctctcacac ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa
    23461 acgatattat gcatacgggc gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg
    23521 acgcattgct ggaagttacc cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata
    23581 tgatgattga aggcatcttt accgacggtg atttacgccg tgtcttcgat atgggcgtgg
    23641 atgttcgtca gttaagtatt gccgatgtga tgacgccggg gggaatacgc gtgcgcccag
    23701 gcattctggc cgttgaggca ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg
    23761 ttgccgatgg cgaccattta ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg
    23821 tagtgtaaag attcaaggat aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg
    23881 ttacggccct gtcagcgccg acgttatggc aaaagcagag aacattcgtc tgctgatcct
    23941 cgacgttgat ggcgtactgt cagatggcct gatttatatg ggcaataacg gcgaagagct
    24001 gaaagcgttc aatgttcgtg acggttatgg cattcgttgt gcgctcacct ctgatattga
    24061 agtcgctatc attaccgggc gaaaggctaa actggtagaa gatcgttgtg ccacattggg
    24121 gatcactcac ttgtatcagg ggcagtcaaa caaactgatc gcctttagcg atctgctgga
    24181 aaaactggcg attgccccgg aaaatgtggc ttatgtcggc gatgatctca tcgactggcc
    24241 ggtaatggaa aaagtgggtt taagcgtcgc cgtggccgat gcgcatccac tgttgatccc
    24301 gcgcgccgat tacgtaacgc acattgctgg cggtcgtggc gcagtgcgag aagtttgcga
    24361 cttattactc ctggcgcagg gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa
    24421 gccagacgtt gggttatcat tgtgctatca ctggcggttc tggtgatgat cggcattaat
    24481 atggccgaaa aagacgatac cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa
    24541 agcgagcata cggacacgct cgtctataac ccagaagggg cactaagcta tcgattgatt
    24601 gctcaacacg ttgaatatta ttccgatcag gccgtttcgt ggtttacgca gccggtactt
    24661 accacgtttg ataaggataa aatcccgaca tggtccgtaa aagcagataa agccaagctg
    24721 accaatgacc ggatgctcta tttatatgga cacgttgaag tcaatgcact cgtgccggac
    24781 tctcaacttc gcagaatcac gacggataac gcgcagatca atctggtgac gcaggatgtt
    24841 acctctgaag acctcgtcac gttatacgga acaacattta actccagcgg tctgaaaatg
    24901 cgcggcaact tacgcagcaa gaacgccgag ctgattgaaa aggttagaac atcctatgaa
    24961 attcaaaaca aacaaactca gccttaatct tgtgcttgcc agctcacttc tggccgccag
    25021 cattccggca tttgccgtaa ccggagacac tgatcagccg atccacattg aatcggacca
    25081 gcaatctctt gatatgcaag gcaacgtggt tacctttacc ggcaatgtca tcgtcaccca
    25141 gggcaccatc aaaattaatg ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg
    25201 taaagaagtg attgacggct acggtaaacc ggcaacgttc taccagatgc aggacaacgg
    25261 taaacccgtt gaaggtcacg cttcacagat gcactacgaa ctggcaaaag attttgtcgt
    25321 tctgacgggt aatgcttatc tgcagcaggt cgatagcaac attaagggcg ataagatcac
    25381 ttacctggtg aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac
    25441 cgttctggtg ccgtcgcagc tgcaggacaa aaacaataaa ggccagaccc cggcacagaa
    25501 gaagggtaat taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa
    25561 aggccgtcgc gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct
    25621 gctggggcca aacggtgccg gtaagaccac cactttctac atggttgttg gcattgtgcc
    25681 gcgcgatgcg ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc
    25741 acgcgcgcgc cgcggtatcg gctatctgcc gcaggaagcc tccattttcc gtcgcctcag
    25801 cgtttacgat aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg
    25861 tgaagaccgc gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat
    25921 ggggcagtca ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc
    25981 gaatccgaaa tttattctgc tcgacgaacc gtttgccggg gttgacccga tctcggttat
    26041 cgacattaaa cgcatcattg agcacctgcg cgacagcggc ctgggcgtac tgatcactga
    26101 ccacaacgtg cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca
    26161 tttgatcgcc cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata
    26221 ccttggggaa gacttcagac tctgataggg tagaagtttg cgacgtttta gcaggagagt
    26281 acgattctga acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg
    26341 ccacagctcc aacaggcaat tcgtctgttg cagttgtcga cgctggaact tcagcaggag
    26401 ctacagcagg cgctggagag caatccgctg cttgagcaaa tcgacactca tgaagaaatc
    26461 gacacccgcg aaacgcaaga cagtgaaaca ctggacaccg ccgacgcgct cgaacaaaaa
    26521 gagatgccgg aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca
    26581 ccatccggca ccagcggtga ctacattgac gacgagctgc cggtctatca gggcgaaacg
    26641 acacagacct tacaggatta cctgatgtgg caggtcgagt tgacaccgtt ttccgacact
    26701 gaccgcgcta ttgctacctc tatcgtcgat gccgttgatg acaccggtta tctgactgtc
    26761 ccgctggaag atattctcga aagtatgggc gatgaagaga ttgacatcga cgaagttgaa
    26821 gccgtcctta agcggatcca aaggtttgat ccggtcggtg tggcggcaaa agatctgcgt
    26881 gactgcctgc tgatccaact ctcccaattc gataagacca cgccatggct ggaagaggcc
    26941 agactgatca ttagcgatca tctcgatcta ttagccaatc acgacttccg cactttaatg
    27001 cgcgtcacgc gtctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc
    27061 gatccgcgcc ccgggcagtc gatccagact ggcgaacctg agtatgtcat tccagatgtg
    27121 ctggtgcgta agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt
    27181 ttgcaaatca accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgatagc
    27241 cagtttatcc gcagcaatct gcaggatgcc aaatggttga tcaagagtct ggaaagccgt
    27301 aacgatacgc tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag
    27361 caaggcgaag aatatatgaa accgatggta ctggctgata tcgcccaggc cgtcgagatg
    27421 catgaatcga cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt
    27481 tttgaactga agtatttctt ttccagtcac gtcaatactg aaggcggggg tgaagcctct
    27541 tccacagcga ttcgcgcact ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg
    27601 ttgagcgaca gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggcacgccgc
    27661 actgttgcga agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc
    27721 gtttgaccca accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga
    27781 gatcaccgag gcactgcgcg aatttgttac agccaaattt gccaaacttg agcaatattt
    27841 tgaccgaatc aaccaggtct atgttgttct gaaagtggag aaggtcaccc acacctcaga
    27901 tgcaacactg catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta
    27961 cgctgccatt gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa
    28021 actgaaacaa cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca
    28081 caacgggcca tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt
    28141 cttaggtgaa attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag
    28201 ggaatgtacg cgaagccgcg tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag
    28261 cgagctggca gcgaaacaac ttagcctggc gccccaggtg gtttttgaag ctatcctgac
    28321 gcgggaaaaa atgggcagta ccggtatcgg caatggtatt gccattccgc atggcaaact
    28381 ggaagaagat actctgcgtg ccgttggcgt tttcgttcag ctcgaaacac ctatagcttt
    28441 cgatgccatc gacaaccaac cggtggactt actttttgcc ctgctggtgc cggcagacca
    28501 aactaaaacg cacctgcata ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat
    28561 ctgccgccgt ttacgcgcag ctcagagcga tgaagagctg tatcaaatca ttacggatac
    28621 cgaaggtact ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga
    28681 gaaacagtac atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc
    28741 cctgcgtgcg ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt
    28801 acccgatctg gctcgaactc tggccgatcg agagatttct gccgccgtca gcattgatgt
    28861 tcgtaatatg ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc
    28921 tttctcaccg caactactgt tcctggatgc cgaccgtaat accttaattc gtcgttacag
    28981 tgacacgcgc cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga
    29041 caaagaaagc gatttgctgg agcctctgcg ttcgcgagcg gatctgattg tcgatacctc
    29101 agaaatgtcc gttcacgagc tggcagaaat gctgcgtacc cgtctgctgg gtaaacgtga
    29161 acgtgaactg accatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc
    29221 agattacgtc tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgccc
    29281 aatgacaggt cttgataaac ctgtcgccgc gttcctcgac cgccacacag aagtacacaa
    29341 ttttatctac cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa
    29401 ccgtagctac ctgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat
    29461 tgcagagcaa ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg
    29521 gacgctggaa aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg
    29581 ggcatgcatg cccggcctgc aatgaagctg tttgaattaa tgcagggttt tgacgctgaa
    29641 gtgctgttac gtaatgacga aggcaccgag gctgaagcca acagcgttat tgcgctgctg
    29701 atgttggatt ctgccaaagg acggcagatt gaagttgaag cgaccggtcc acaggaagag
    29761 gaagcactgg ccgccgttat cgccctcttt aactctggtt ttgatgaaga ttaatcttca
    29821 tcactttgac attcaaatcc cttcaagctc ccccggcaat aagaaaagcg cgtactgaaa
    29881 tcgttatcaa tcaacgtcga ttgtacattt cgtgcgcttt ttactttcag aagaacctta
    29941 agaaaacctt aagaagcatt gtttagattt cgtttaagtt aatcgaccat actggagatc
    30001 gtcagaaaat atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca
    30061 ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt atcaagattg
    30121 tctaccatcg tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgc
    30181 atcttggtcg tcggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga
    30241 cggattgcgg aaccggttat gtctacgatg tgattgccga tttcgacggc aagccttcca
    30301 ttacgctgac cgaatttgca gaacaatgcc gttacgaaga agacattgca caactgcgcc
    30361 agttactgaa acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga
    30421 agccgcagaa tatcctctgt caccgcatca gtgagtcaga ggttaccccc gtcgtctgcg
    30481 acaacattgg tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc
    30541 gtaagcaaga gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc
    30601 agaaagatct gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat
    30661 caagctggtg ttgttgcata aacggctcac cgcctagctg atacatctgc cgtaaaatcc
    30721 acgcctgacg gctacgcacg tagcccgatg gcgcggagac tttgaaacga agtggattag
    30781 gtaatacagc tgccagcaat gcagcttccg accgggtaag tttgctcgcg ggtttgtgga
    30841 aataacgttg tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca
    30901 ggtaaaccgt caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccgg
    30961 cttccagccc ttttcgaacc cagctacgcc catcccataa aaagagattt ttggctgtct
    31021 gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg tgcgccatgg
    31081 cttgctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga tcttctgcgg
    31141 caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg
    31201 ccacgtaacg aaaattgcca tgcagccagg cgctgacttg ccgctcgacc attactgctg
    31261 agaaaggaac aggcgcaacg ctaaacaacg cgatgccccc gccccagaaa atggcgagga
    31321 caaccattaa ccgcagaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac
    31381 tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc
    31441 aatgttctgc gccagcatat atgccggggt ggtgacaatc ttattgtctt catcaaccac
    31501 gatatcatcg acaggacacg gcacatgctc cgcgcccatc tcttccagca cttctgcggt
    31561 atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg gcagtatcgc
    31621 cggtgctata cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc
    31681 ctttaattca cggtcaacgg tgcattcgct accaagactg gcaaaattgc ttaaattctt
    31741 cgccgcgcca aaaccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc
    31801 cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacat tgcgcgtttc
    31861 cgtcatcgct tcaccagtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa
    31921 gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat
    31981 ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat
    32041 cttcctttcg caattgactg aaacacatta atttttttaa taaaaatgct acgcatcaca
    32101 catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct
    32161 gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc
    32221 cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt
    32281 ttgccaccca cgctttcagt acttctacgt cgtgacgcca ctcttctttc atctcttcaa
    32341 tccattcacc gacgttatct tcccaggcgg gaaggtcagg agactgaatt tgctgaccca
    32401 gctgttgcag atggcgcaac cccactgacc ccgccgcacc tttaatttta tgcccttcct
    32461 caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg
    32521 gcatcatcct ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagttcga
    32581 gatactgttc cagcatggga atatcgagca atgcttctgc tttactgttc tcttctgtcg
    32641 tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg
    32701 ccggaacaga aagcggctta ctcagcacat catccattcc agcattgagg tactcttgtt
    32761 tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac
    32821 gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca
    32881 acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga
    32941 catcaacgct gttacctaac ttttccagca cagagcgcgc gacaatcacg ttcagttcaa
    33001 tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat
    33061 catcaacctc ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc
    33121 cctgttcgct ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg
    33181 ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat
    33241 acatggcgaa gattttatcc agctcatcct gcggaatgcc gataccagag tcttccactt
    33301 caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctgag
    33361 tgaatttgac ggcgttactg atgaggttcc acaagatctg ccgtaaacgc gtcccgtcgg
    33421 taatgacctg atgcggtaat ggcagcgtcg gctccagatt aaagcgcaat cctttttgtt
    33481 gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggct
    33541 gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata tcgttgaaga
    33601 tattccccaa cgtgacagcc gaaacatgga tggttttgag atatttttcc tgctcggcgg
    33661 tgagttcagt atccagcaga atgcggctca ggccgacgat accgttcagc ggtgtacgca
    33721 attcgtgact gatggtggag ataaacgtcg ttttgtcgcg gctggcgcgc tcaagcgcat
    33781 cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac
    33841 ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgt ccgtcgggat
    33901 aatccagcca ctgctcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt
    33961 caatgacttt cgcagcggct tccggtgagt aaacatcagc aggtttcagg tgaaccagtt
    34021 gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat
    34081 cttcgttacg ataaaaaacc aggtcgggtg aagcgtcaag gaaggaacgt aagaatgagg
    34141 attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc
    34201 cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcgatttcc tgatttagct
    34261 gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca
    34321 gtttttgcac cagtcgtgac agacgttgtc gtgactcctc cagttgctcg acgaccaccg
    34381 atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat
    34441 caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc acaatggcaa
    34501 gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac ttcatcatca
    34561 ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc cttcacgaca
    34621 acctgcccca ataataccca attctgacag ttacgttata aattgtgtga gaaatgcgga
    34681 attcccctca atggcctacg ccgttggagg tagccacgga cgtcccagcg ccgatccctg
    34741 tacgccatgt tcattcagat aacgatccag ctcgaccatt cccgtccatc gattttcgca
    34801 ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac
    34861 ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtgtaatcct ccagttcaat
    34921 tccgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca ctatatgcag
    34981 cggatgcagc tttatgccgt ccacgccggt ttcaaccact cgctccagcg tttgcaaaca
    35041 ttcagcctgc ccttcgccag gcaggccaac aattaagtga gaacacacct tcagcccacg
    35101 ctcacgcgcc agctgcgtcg tgcgctgata gcaggcaaaa tcatgaccgc gattgatgcg
    35161 atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt cgtagccctg
    35221 gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg ggcgggtacc
    35281 aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatcg aacgcagcac
    35341 ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc gtttagcgcg
    35401 gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc
    35461 atcggcaaac gaggcaacat tacagaatgt gcagccgcca cgcccgatgg taccgtcacg
    35521 gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc cataacgacg
    35581 ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct gatagaccgc
    35641 gccttgaaaa gaggccaaag cctgccattt ttagcccaat tcggcgatga cctggatcaa
    35701 tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa tttcatgcac
    35761 aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct tatggatttt
    35821 aggcaaaaac agtagcatga aacgttatta ccaattaagg cagtataaaa tgctggtttt
    35881 ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag gtattagctg
    35941 tttctctaac gacgcccctt ttagccttaa atagaaaatc caatttaatt tcagataatt
    36001 aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat ttgacctgta
    36061 tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca gcctgctcat
    36121 catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa
    36181 caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac gctcttactg
    36241 tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag gttcacgata
    36301 tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc gcccacatag
    36361 aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc
    36421 accgtggcgc gattctcgcc gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa
    36481 aaccggatcg tttttttcgc atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact
    36541 acgccgtcgg gatgctcttc ctgaataaag atcctgaact cgccgctgcc gcacgccgca
    36601 tcgttgaaga agagctgcaa cgcgaaacgt tgtcgattgt gggctggcgt gatgtcccca
    36661 ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt gagcaaattt
    36721 ttgtgaacgc cccggcaggc tggcgtccgc gcgatatgga gcgccgtctg tttatcgccc
    36781 gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt agcctgtcga
    36841 atctggtcaa tatttataaa ggtctgtgta tgccggcgga tctgccgcgc ttctacctgg
    36901 acctcgctga cctgcgtctg gaatcggcca tctgcctgtt tcaccagcgc ttttccacta
    36961 acaccgtacc gcgctggccg ctggcgcagc cgttccgcta cctcgcgcat aacggtgaaa
    37021 tcaacaccat cactggtaac cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc
    37081 cgctgatccc tgacctgcac gacgccgcgc cgttcgtcaa cgaaaccggc tctgactcca
    37141 gttccatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc atccgcgcca
    37201 tgcgtctgtt agtaccaccc gcctggcaga acaacccgga tatggacccg gaactgcgtg
    37261 ccttctttga ctttaactcc atgcatatgg agccgtggga tggcccggca gggatcgtga
    37321 tgtccgatgg ccgtttcgcc gcctgtaacc tcgaccgtaa cggtctgcgt ccggcacgct
    37381 acgtcatcac caaagataaa ctgatcacct gcgcctctga agtcggtatc tgggattatc
    37441 agcctgacga agtggtcgaa aaaggccgcg tagggccagg cgaactgatg gtgatcgaca
    37501 ctcgcagtgg gcgcattcta cactcggcag aaaccgatga tgacctgaaa agccgccatc
    37561 cctataaaga gtggatggag aaaaacgtcc gccgtctggt accgtttgaa gatctgcccg
    37621 atgaagaagt gggtagccgc gaactggacg acgacacgct tgccagctac cagaaacaat
    37681 ttaactacag cgcagaagag ctggattcgg tgatccgcgt actgggtgaa aacggtcagg
    37741 aagcggtcgg ttcgatgggc gacgacaccc cattcgccgt gctctccagc cagccgcgca
    37801 ttatttacga ctacttccgc cagcagtttg ctcaggtaac caacccgcca atcgacccgc
    37861 tgcgcgaagc gcatgttatg tcgctcgcca ccagcattgg ccgcgaaatg aacgtcttct
    37921 gcgaagcaga gggacaggcg caccgtttaa gctttaaatc gccgattctg ctctactccg
    37981 atttcaaaca gctcacgacg atgaaagagg atcactaccg cgcagatacg ctggatatca
    38041 cctttgacgt cactaaaacc acgctcgaag cgacggtgaa agaactgtgt gacaaagccg
    38101 aaaaaatggt acgtagcggc accgtgctgc tggtgctctc cgaccggaat atcgctaaag
    38161 atcgcctgcc ggttccggct ccgatggcgg ttggcgcgat ccagacccgt cttgtcgagc
    38221 aaagcctgcg ttgcgatgcc aacatcatcg tagaaaccgc cagcgcccgt gatccgcacc
    38281 acttcgccgt gttgctgggc ttcggtgcga cggctattta tccgtacctt gcctatgaaa
    38341 cgctgggccg cctggtagac acccacgcga ttgccaaaga ttatcgtacc gtgatgctca
    38401 actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca
    38461 ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctgcac gatgatgtcg
    38521 taggcctgtg cttccagggc gcggttagcc gcattggcgg ggcgagcttt gaagacttcc
    38581 agcaggatct gctgaatctg tcgaaacgtg cctggctggc gcgtaagccc atcagccagg
    38641 gcggcttgct gaaatacgtg cacggtggcg aataccacgc ctacaacccg gacgttgtgc
    38701 gcacactgca acaggcggta caaagcggcg agtacagcga ctatcaggaa tacgcgaagc
    38761 tggttaatga gcgtccggca accacgctac gcgatctgct ggcaattacg ccgggtgaaa
    38821 acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc ttcgataccg
    38881 ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg gaagcgatga
    38941 acagcatcgg cggtaactca aactccggcg aaggcggtga agacccggcg cgctatggca
    39001 ctaacaaagt gtcgcgcatc aagcaggtgg cgtccggtcg cttcggcgtt actccggcgt
    39061 acctggtcaa tgctgacgtg attcagatta aagtcgccca gggcgcgaag ccgggcgaag
    39121 gcggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc tattcggtgc
    39181 ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc gaggacttag
    39241 cgcagctcat tttcgacctc aagcaggtta acccgaaagc gatgatctcc gtgaagctgg
    39301 tgtccgaacc gggcgtcggc accatcgcga ccggcgtggc gaaagcttat gcggacttga
    39361 tcaccatcgc aggctatgac ggcggcaccg gcgcaagtcc gctttcatcg gtgaaatacg
    39421 caggctgtcc gtgggaactc ggcctggtgg aaacccaaca ggcgctggtt gccaacggtt
    39481 tacgtcataa aattcgtttg caggttgatg gcggcctgaa aacgggcgtt gatatcatca
    39541 aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg gtcgcgctcg
    39601 gttgtaaata tctgcgaatt tgccacctga acaactgcgc cacgggtgta gcaactcagg
    39661 atgacaaact gcgtaagaat cactatcacg gcctgccatt caaggtgacg aattactttg
    39721 aatttatcgc ccgtgaaacc cgcgagctga tggcacagct gggggtaacg cgtctggtgg
    39781 atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc aaacagcaga
    39841 aactggcgct gtcgaagctg ctggaaactg ccgagccaca tgcaggtaag gcactctact
    39901 gcaccgaaaa caacccgccg tttgataacg gcctactgaa cgcgcagttg ctgcaacagg
    39961 caaaaccgtt tgttgatgaa cgccagagca aaaccttctg gttcgatatc cgcaacaccg
    40021 accgttctgt cggcgcatcg ctttcaggct atatcgccca gacgcacggc gatcaggggc
    40081 tggcagccga tccgatcaaa gcgtacttca acggcaccgc aggccagagc ttcggcgtgt
    40141 ggaacgcggg cggcgtggaa ctgtacctga ccggtgatgc caacgactat gtcggtaaag
    40201 gcatggcggg tggcttaatc gccattcgtc ctccggttgg ctcagccttc cgcagccatg
    40261 aagcaagcat tatcggcaac acctgcctgt atggcgcgac cggaggtcgt ctgtatgccg
    40321 caggccgcgc gggtgaacgt ttcggcgtgc gtaactccgg tgctatcacc gtggtagaag
    40381 gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt ctgggtaaaa
    40441 ccggcgttaa cttcggcgcg ggcatgaccg gtggcttcgc ttacgttctc gatgaaagcg
    40501 gcgatttccg caaacgcgtt aacccggaac tggtcgaggt cttaagcgtt gacgatttgg
    40561 cgatccatga agagcatctg cgtggtctta tcaccgagca tgtgcagcat accggttccc
    40621 agcgcggtga agagattctg gcgaactggt caaccttcgc cacgaaattc gcgctggtta
    40681 aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc gcagcagagt
    40741 tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat caatttatcg
    40801 acctgcagcg cgttgatccg ccaaagaaac cgctgaagat ccgcaaaatt gagtttgttg
    40861 aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt
    40921 gcggcaaccc atactgcgag tggaaatgcc cggtacacaa ctacatccca aactggctga
    40981 agctcgccaa tgaggggcgt atttttgaag cggcggaact gtcgcaccag acaaacaccc
    41041 tgccggaagt ttgcggacgt gtctgcccgc aagaccgtct gtgcgaaggt tcctgcaccc
    41101 tgaacgatga gttcggtgcg gtgaccatcg gcaacattga gcgttatatc aatgataaag
    41161 cgttcgagat gggctggcgt ccggatatgt ccggcgtgaa acagaccggt aaaaaagtgg
    41221 cgattatcgg cgcaggtccg gcgggtctgg cgtgcgcgga tgtcctgacg cgcaacggcg
    41281 taaaagcgat cgtcttcgac cgtcacccgg aaattggcgg cttgctgacc ttcggtattc
    41341 cggctttcaa gctggaaaaa gaggtaatga cgcgccgccg tgaaatcttc accggcatgg
    41401 gtattgaatt caaactcaat accgaagtgg gccgcgacgt gcagctggac gatctgctga
    41461 gtgattacga tgccgtgttc cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg
    41521 aaaatgaaga cgccgatggt gtgtacgcgg cgctaccgtt cctcatcgcc aacaccaaac
    41581 agttaatggg ctttggcgaa acccgcgacg aaccgttcgt cagcatggaa ggtaaacgcg
    41641 tggtggtcct tggcggtggc gacactgcga tggactgcgt gcgcacgtct gtacgccagg
    41701 gggcgaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc
    41761 gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc cagccgctgg
    41821 gtatagaagt gaatggcaac ggcaaagtca gcggcgtaaa aatggtgcgc acagaaatgg
    41881 gcgaaccgga tgccaaaggc cgtcgccgcg cggagattgt ggcaggttcc gaacatatcg
    41941 ttccggcaga tgcggtgatc atggcgtttg gtttccgtcc acacaacatg gaatggctgg
    42001 caaaacacag cgtcgagctg gattcgcagg ggcgcattat cgccccggaa gacaacgaca
    42061 acgctttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg
    42121 atctggtggt gaccgctatt gccgaaggtc gtaaggcggc agacggtatt atgaactggc
    42181 tggaagttta agcctgtccc agcgccgcct cctcagaaaa gagtcggcgc tgaaattctc
    42241 ctttctttct ggaaagcgcc tcgcataatc tgaagaaaac caatgtaata cgctcatctg
    42301 gattgttacc ggtagatgcc cggcgtatag ctgattccat tattcaacgc agcgccatat
    42361 aaagggacag ataatggaat cgctctcaga gagaacctca acaggctacc agcaaatcca
    42421 cgacggtatt attcacctgg tcgatagcgc ccggacggaa acggtacgta gcgttaacgc
    42481 gctgatgacc gcgacatact gggaaattgg ccgacgaatt gtcgaatttg aacaaggtgg
    42541 cgaggccagg gctgcgtatg gtgcgcagct aatcaagcga ctatcaaagg atttaagtct
    42601 aaggtataag cgtgggttct ctgcaaaaaa cttacgccaa atgaggcttt tttacctctt
    42661 ttttcaacat gttgaaattc gccagacagt gtctggcgaa ttaacaccat tgggaattcc
    42721 gcagacacca tctgcagaat tcccatcggc aaagatttgg cagacgctgt ctgccaaatc
    42781 tttccccctg ccctggtcca cctacgtccg tttactttct gttaaaaacc ctgatgcccg
    42841 cagcttttat gaaaaagaga cgctccgctg tggttggtct gtccgccagc tagagcggca
    42901 aattacgacc caattttatg agcgaacact actgtcacat gacaaatcag ccatgctgca
    42961 acaacacgct cctgccgaga tgcatattct tccgcaacag gcgatacgcg atccctttgt
    43021 gctcgaattt ctggagttga aagatgaata ctcagaatct gattttgagg aggcgctgat
    43081 caaccacctg atggatttca tgctggaact tggggatgat tttgcctttg ttggtcggca
    43141 gcgaaggtta cgcattgatg acaactggtt tcgggtcgat ctgctgtttt tccaccgccg
    43201 tttacgctgc ctgctaatcg tcgatctaaa agtgggcaaa ttcagctata gcgatgccgg
    43261 acagatgaat atgtatctca actacgccaa agagcactgg acactaccgg atgaaaatcc
    43321 gcccatcggt ctggttctct gtgcagagaa aggagccggc gaagcgcatt atgcactggc
    43381 tggtttgcct aacaccgttc tggcgagcga atataagatg caactacctg atgaaaaacg
    43441 actcgcggat gaactcgttc gaacacaggc ggtgctagag gaaggctata gacgccgtta
    43501 atttcagcgt tttcgaattc tgcagacagt gtctgcagaa tctttaaaac gaactaagtt
    43561 ttaagccatt aaatcagcct taaccttcac cacaaccttt ttaatctcat caggttcgcc
    43621 gacaacgcat cctggtttat gcggttctcc cggcataaac acggcgaaca ttcccggttt
    43681 taagatgatg gcttgctcgt tctcaatggc gctgcaaagc tggtaatcat cctcatggtg
    43741 gaactcttca cactcacgcg cagtgcctgc cgtgccaaac agaatccgtt cctcaccgtt
    43801 taataacagc tggatatcaa tgtattgctc gtgcagctcc gcttttttct caacgggcga
    43861 ttgagtgtta aacgtcatga cattcataaa gatattgtcg ccctgtaatt cgtaacgacc
    43921 cggtgctttt tcttgcggcc tggcggctaa ggctagtgtt aacgcgtcct gtaacgcagg
    43981 atgcaatccg gcagacggta acgactgtac ttcacccatt atcataattt ttctccctgg
    44041 gccaacaacg cagccccaag taaacctgca tcgtggcggt agtgcgccgc cagtaaatca
    44101 acatgaaatg ccgctggctc ctgcgccaga tacgtttcca ccagcgccag atacccttct
    44161 gccagaccaa cgctgccacc gaccaccacg cactggcaat cagttgtggc tttaatatca
    44221 gcgatcagcc ttgcaagcgt acgtgcggag cggtgaatca gttgctgcgc ctgctcatca
    44281 ccctgcccgg cgcgcgtgaa aatagttttc gcattcgcac cagccaactc cccctgcgct
    44341 gccgttgcaa tgccgcgacc agaagcaatc gcttccacgc aacctgtgcg tccacagccg
    44401 cagactggac catgcggatc ggcaagcgta tgcccgatat gccccgccag accgccaggg
    44461 ccggtacgca gtttaccgcc gctcactaca ccgccgccaa cgccggtgga aacagtgata
    44521 aacaccatat cggttatatc gccatccagc gcctgatact ccgcccatgc tgcggcctgc
    44581 gcatcattaa tggcaatggt cggcaaattg gtaagttgct ccagcgtttt gactaacgga
    44641 aagtgtaaca atccaccgag attatgcgga ttcagcgcca acaagctgcc gtcgcgaata
    44701 attccggttg aagcgatggc aacccgctgc gcatgtgctt gcaacggaga gactaatgcg
    44761 gataaggcat cacgcagcgc ctgcggagtc tggctggcag gcgtaggaag ttctcggcga
    44821 tcgcggatct gcccgtcagc gccaatcagc gcggcggcaa gtttagtacc gccgatatca
    44881 atcgccagtg tggtcatagc accgcctttt tcatcgctgt gttgtaccac tgacaaatgt
    44941 gttcaagacg cgtaatggca gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat
    45001 ccgccgcctg agcaggcgtg ttgtaacgcc cttcggcaat cacccggcat ccggcgtcgc
    45061 tcaacgtttt caccagcgcc agatccggct cttctggcgt ttcaggcgtg gtatagccag
    45121 aaagcgtagt gccaataatt tcggctccca gcttttggca tgccaggccg tcttccggcg
    45181 ttgagcagtc ggtcatcgcc agtaaaccgt gatggtgaat acgtgccagc agcgtttcaa
    45241 ctggcaccgg acgcggtcgg tcggtgccgt caatcgcgat aatgtccgcg cccgcctgcg
    45301 ccagcgcatc aacatcttca atataggccg tgatgcgtac cggagaatcc tccagatcgc
    45361 gcttcacaat cccaataatc ggcacgctca ccaccgcacg cgtggcttgc agatttgcca
    45421 caccttcaat acgaatggcg accgcgcccg cctgctctgc cgctaatgcc atggcagcga
    45481 cgatttcggg tttatcgagc gggctgtccg gaaccggctg gcaggagaca atcaggccac
    45541 cgttagcagc gattttttga tccagttgtg caagtaacga catacatctt cccttagcga
    45601 aaggcccggt acagagaccg ggcaacagga ttaacttttg gttttgacta aattgttttt
    45661 ggcgctgcca aatggcacgg caccgctaaa tggtttgccg tcgatagcgt catgagtacg
    45721 caatgcttcc gggcgcagcc aacgctgaac acgagaaggc atatccagac caatcagcag
    45781 gatcaccaca aatgtcagac tgaacgagag cgatgccagc gcagtaccca gatccagacg
    45841 ttgagcgatc aacgcgccga tgattggggc cagtgcaccg cccaatgcgc caacgttgta
    45901 ggtaaagccc aggcccgctg cacgctggtc ggtatcgaaa taaccgccaa tcagtttcgg
    45961 taagatcccg gcgatccctt gtccaagcat ttgctggaag aacagtaaca gaccgagcac
    46021 ccagacgttt gcgccgccaa tcgcaaatac cggaataatc agcagctgcg aggccagcag
    46081 actacaaacg tacgctttgc gggttcccag ccagtcaccg aggaagcccc cgacgcagca
    46141 ccccaccgcc gcgccaaagc cactaaagaa cagcacattg gcgacagtat gcgggtcata
    46201 agccagatcg gttttcagat acgttggcag cagcgcctga atcggccatg agtagaggaa
    46261 agcaaacaac acgaccacca tcagcattac gcccgttggc cagcgtttgc ccgtactctg
    46321 caccataaag ctgataaaaa ttgcggcgca taacagccca agaacagcga cgatcgcggc
    46381 attttgcagg ttaccggcga agcagaacca cagcgcagta gccgccgcca gtgtcattac
    46441 gatattggca atgcgatgtt caccacggta gagaatgtct accatcgtgc gtactggtgc
    46501 tttacccccg tgtttctctt tccagtcttc cgcttccggg atgtttttac gcagccagag
    46561 agcaaagatg attggcaaaa tgccgataaa gaacagcgca cgccagcccc agaccggaac
    46621 caccagacta tagacctgag cggcaacgac ggcccccact gagaagcctg aaatcagaaa
    46681 accactggct ttgttacgca gatgctttgg ccagctttca atgacatagg tggcgctgga
    46741 accgtattca cccgccatcc ccatgccgat gaccagacga gcgataaaca tggtgatgta
    46801 gcctggcgca aagccgcagg ccagcgtccc ggccgagaag agaacgatgc tggtgaccat
    46861 tgccagacga cgcccgtagc ggtcacccat agcgccgagc atcaggccgc cgaaccagcg
    46921 agagataaag gctgcggaga tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc
    46981 ctgtacttcg gtgagtacca gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc
    47041 caaccaggca gcggaaaatg cccgccattg tgcgcggtta agatggcgat accacgggat
    47101 attctgggtt gtagtactca ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg
    47161 tatgaaaatt gtagggtaca gatgcgttta tttcccctca cccggtaggg gcgagcgagg
    47221 ggaaacaact cacccgcgct cttgcatcaa ctgctgggcc agcgccttca gttctggcag
    47281 atatttttca tctaccggac caaacggttt gcggcacagc ggcacagaaa cgacatccat
    47341 ataatgcagg acagttttca ggccgcggaa tacgcccgtt ttgatcagta aatcaatgac
    47401 tttgttgcac tcagtttgca gtttctgcgc ggtctggata tcgccttctt tcagcgcctt
    47461 cacgatcccc tgataacgcc agcccatgat gttgtaagtg ctgccgatac cgccgtctgc
    47521 gcccgccaga agaccagagg cgaagatttc gtcgtaaccg ttatagagca ccagatcagg
    47581 atgttcacga cggatctgct ccatctgata gagatcgcca gaggtctgtt tcagcgcacc
    47641 tacaccaggc agcgtaacca gggtgttgat ctgatccagg gtcagtttta ccccgctcag
    47701 ggctggaatg ttgtacacca ccatcggcaa cccatccgcc gaatcaataa ttgcccgata
    47761 gtgatcgcag tgttcttcaa agctgaaagg atagtagaac ggcgtgacgg cggagacggc
    47821 atcgaagcca taacgtttag ccgatgccgc aagttgttgg ctttcggcgg tgctgacgca
    47881 accgacgtgg gcgatgagtt taatcttacc tttcgcctct tcagcgacga tttccagtac
    47941 ctgttcacgc tctgaaaggc tctgtacaaa ggcttcgcct gtcgaacccc ccacgtacaa
    48001 accgtcgatg ccctgctgaa tattgaactg aaccaagcgg cgcagactcg ctttatccag
    48061 tgcttgttgt tggtcaaaag gagtcaggag tgcagccatt acgccacgta aattcgttgc
    48121 cataaatacc tctgaagtga tgcttgtctg ataaacgata tacctttata cctgttatac
    48181 cagatcaatt aagcaacacc ccatacagaa agcttataat gcgatctgct tcactaaagt
    48241 ggcattattt ctttttgttg gtggtctgac cgaaggcgtg ccaggtagca gagacgctgt
    48301 tgagatgcga ttgcaacgcg cgatcggctt cgtcaggatc atgacggcgg atcgcgtcaa
    48361 cgatcgcaat atgctgttga tagctaacgt tgttatgctc atgcagagcc tgatcggcca
    48421 cggttggacg tgcggcgatt agccagtcga gcagggcaac gtggatcgcc atgaagattg
    48481 ggttaccagg gatctccgcc agaacgcggt ggaaatcaac gtctgagcga atgaatgccg
    48541 cgttgttatc cagcgactga ctgttgattt ccagtgcttt tgccagcaaa tcgatttgct
    48601 catcggtggc gtgttcagcc gcatagcgca ccaggctgga ttcaaagaac agacgtaatt
    48661 gttcgaaatg ggcaatccca ccaggatgag aaaggaaatc tttcgccatg ccggaaagct
    48721 cgccgatgat ggtgtccgca gaaggacgtg agacgcgggc acgttcgccg ttgtttattt
    48781 gcaccagacc tttgcgtttt aacgctgcca gcgcttcgcg caccgaagga cgcccgacgt
    48841 taaagaacgc catcagttcg cgttcagacg gtaattgttc accttcgcca aattcacgac
    48901 ggcggatcat ctgttccagc tcttcttcca ccatttcgga gagtttttta cgcgccagcg
    48961 ggcggctgcg caagttgcgc ccgatagtgg tagtagaatc ttcagcttgc ggatcaaatg
    49021 cgttcatagg actcattcag gaaaatgcgt tagcggataa taacgacgct atcgtagcac
    49081 agtatcaaag tcaggcgaaa agatgacaga tagcaaaaca ggccacatgg gcctgttttc
    49141 attttacttc acaacgcgta atgccggtcg accaccgcgt ggtggctgcg gaggttcatc
    49201 gtcaggatga gtttcatcat cgtgatctgg cttgtcgcca tcgataaccg acataacggt
    49261 ttcgttgtct gccgatgcct cttcatcatt catgatgctg gcatcttcat cgtaggcagc
    49321 ttcaggctca aacatcgtgc ctgcaccatt ttcacgggcg tagatagcca gcacggcagc
    49381 cagcggcaca gaaacctgac gcggaatgcc accaaagcgc gcgttaaagc gcacctcatc
    49441 attcgccagt tccagattgc cgacagcacg cggcgcaatg ttgagtacga tttgcccgtc
    49501 acgcgcatat tccataggaa cctgcacgcc tgggagcgtc acatccacca ccaggtgcgg
    49561 cgtgagctgg ttatccagca accactcata gaatgcacgc agcagatagg gacgacgtgg
    49621 tgttagctgt gacaaatcca tacagattaa ctccggccca gacgcatttc acgttctgct
    49681 tcagttaaag aagcaaggaa agagtcacgt tcaaagacgc gggtcatata gcctttcagc
    49741 tctttcgcac ccgggccgct gaactcgatg cccagttgcg gcagacgcca cagcagagga
    49801 gcaaggtagc aatcgaccag gctgaactca tcgctcagga agtacggctt ctgaccgaag
    49861 accggcgcaa tcgccagcag ttcttcgcgc agttgcttac gtgcggcatc ggcttcagaa
    49921 gctgaaccgt tgatgatggt gttcatcagc gtgtaccagt ctttttcgat gcgatgcatg
    49981 tacagacggc tttcaccgcg agctaccggg taaacaggca tcagtggcgg atgcgggaaa
    50041 cgctcatcca gatattccat aatgatgcga gattcccaca gggtcagctc acgatccacc
    50101 agggtcggaa cgctctgatt cgggttgagg tcaatcagat cctgaggcgg attgtccttt
    50161 tccacgtgtt cgatctcgaa acttacacct ttctcagcca gcacaatgcg gacctgatgg
    50221 ctatagatgt cagtaggacc ggaaaacagc gtcattaccg aacgtttgtt ggcagcgaca
    50281 gccatgaaaa cctccaggta tagtcagaat ttttactgct accagccacc aggtggccag
    50341 tcagaagttg tgttacccag taaggaacga ctctctttgt tcgaaaatca aacaaaaaat
    50401 gagcaatacc cgacatttgg gcagaaaatt ggatgatagt ttaccagatt ttgcgacctt
    50461 tgtggtgagt cgatgccgga aatggggaaa aagagatgcg ctttagtctg aaatagttga
    50521 cttagtccct tattggcgat gtggtttttg ttttacctgt ctgtcaggtg gcagcaaaaa
    50581 gcaactttcc attttttacg ctgatgcaga ttttagccat aaaaaaaccc gccgaagcgg
    50641 gttttttcgc aaattgtttt ctgccggagc agaagccaat taacgtttgg agaactgcgg
    50701 acgacgacgt gctttacgca gaccgacttt cttacgttca acctgacgag cgtcacgagt
    50761 aacgaagcca gctttacgca gttcagaacg cagggactcg tcgtattcca tcagagcgcg
    50821 ggtgataccg tgacggatcg caccagcctg accagagata ccaccacctt taacggtgat
    50881 gtacaggtcc agtttctcaa ccatgtcgac cagttccagc ggctgacgaa ctaccatgcg
    50941 agcagtttca cgaccgaagt actgttccag agaacgttgg ttgattacga ttttaccgtt
    51001 gcccggtttg atgaaaacgc gagctgcgga acttttgcgg cgaccagtgc cgtagtattg
    51061 attttcagcc attgcctata atcccgatta gatgtcaaga acttgcggtt gctgtgccgc
    51121 gtggttgtgc tcgttacccg cgtaaacttt cagtttacgg aacatagcac gacccagcgg
    51181 gccttttggc aacatgcctt taaccgcgat ttcaatcaca cgctcaggac ggcgagcaat
    51241 catctcttca aaggtcgctt gtttgatacc accgatgtgg ccggtgtggt gatagtacac
    51301 tttgtcagta cgcttgttgc cggttacagc aactttgtca gcgttcagaa cgatgatgta
    51361 atcaccggta tctacgtgcg gagtgtattc cgctttgtgc ttaccgcgca ggcgacgagc
    51421 cagttcagta gccagacggc ccagagtttt accggtcgcg tcaacaacat accagtcgcg
    51481 ttttacggtt tctggtttag ctgtaaaagt tttcattaaa agcttaccca ataaatagtt
    51541 acacgttggt gaacacccaa acgtcttcaa ttgttgaggt tcacacgaca aagtccggca
    51601 aacctacccc ttcgaatagc ctatgccagc acacaaaaag tttcgggaaa aaaaactttc
    51661 ttgtaacgtg gggtcgcagg attatagaga agtcggggtc aaagatcgac ccctttttgt
    51721 gatttgtggc aggttttacc ccgccaaatg ctcgcgcttc aaatactctt cgctttgcat
    51781 ctcttgcaga cgtgacaggc aacgctggaa ctcaaacttc agccgctcgc cctgataaat
    51841 ttcatacagc ggcacttctg cactcaccac taatttgaca tggcgctcgt aaaactcatc
    51901 caccagcgca ataaagcgtc gcgcttcgct ctccatcaac cgcgtcataa ctggtacatc
    51961 aaacaacatg accgtgtgaa agaggcgtga gagcgcaata tagtcatgct gactgcgggc
    52021 gtcgacgcac agcgtagtaa aagaaaccgc cagcgtctgg ttctcgacgc ccattgtcgc
    52081 caatggccga tggttgattt ctaacgtcgg tgaattttct cgtttcgccc ccgccagcgc
    52141 caaccatagt ttatccattt gcgtccggtt ttcatcgttg agtggcgaaa gccacagatg
    52201 cgcctgagtg agtgtacgca gtcgataatc aacaccagcg tccacgttca ttacatcaca
    52261 atgctgttta atggcatcga ttgcaggcaa aaaacgcgca cgttgcaggc catttcgata
    52321 aagttcgtcc ggcggaatat ttgacgtcgc taccagggta ataccgcggg cgaacagggc
    52381 tttcatcaga ccgccaagta gcatggcatc ggtaatatca gaaacaaaaa attcgtcaaa
    52441 acagagcacg tcagtttctg ctttaaagcg atcggcaata atttccagcg gatcggtctg
    52501 cccctgcaaa gcagttagct cttcatgtac ccgcagcata aaacggtgaa agtgcagacg
    52561 ctgtttccgc tctcccggca ggctttgata gaaaaggtcc atcagccagg ttttcccgcg
    52621 tcctacaccg ccccacatat ataagccacg cactggcgta tgctttgtgt cttcgcgttt
    52681 accccacagc ttaccgaccc gcgccattag cccactcgtc ctgggggctg gaggcgtgct
    52741 attgatgagt tcctgataaa taatttccaa gcggctgacg gcctcttttt gaacgtcgtc
    52801 gggttgatgg ctgccttcat tgagcgcctt caggtattgc gatgttgggg taatgctttg
    52861 catgatctta ttgttattcc ttgaataatc ggtgcgccgt tgttcacggt tgacgaaaaa
    52921 aaggccgttc tacactacgc gatatgcagt cgggattcca cttctgtgga attaacggtt
    52981 atagtggcat aatcagccgc aggcatggag cctgaagcca acaccctacg gaaacaaaag
    53041 acaacgggag atgttcatga cctgggaata tgcgctaatt gggttagtcg tcggcatcat
    53101 tattggtgct gtggccatgc gttttggtaa tcgtaaacta cgccagcaac aggcgttgca
    53161 gtacgaactg gaaaagaata aagctgaact ggacgagtat cgcgaagagc tggttagtca
    53221 ctttgcccgc agcgcggaat tactggatac catggcgcac gactatcgcc agctgtatca
    53281 gcacatggcg aaaagctcca gtagcctgct gccggaactg tctgcggaag caaacccgtt
    53341 ccgtaatcgt ctggcagagt ctgaagccag caacgatcag gcaccggtgc agatgcctcg
    53401 cgactattct gaaggcgcat ccggcctgct gcgtactggc gcgaagcgcg actaatttat
    53461 ttttcgggcg cagccattgc gccctcctct tctctccctc cccgactatc atttaatctg
    53521 gtgtctcatt gttagccgtc tgaaaattca ataacatcaa actgttttga atctcttttc
    53581 ttatcattca ggtacgagag caggaataat gaaaaaacaa acccagctgt tgagtgcatt
    53641 agcgttaagt gtcgggttaa ctctctcggc gtcatttcag gccgtcgcgt cgattccagg
    53701 ccaggttgcc gatcaggccc ccctccccag tctggctcca atgctggaaa aagtgcttcc
    53761 ggcagtggtg agcgtacggg tggaaggaac ggccagtcag ggacagaaaa tcccggaaga
    53821 attcaaaaag ttttttggtg atgatttacc ggatcaacct gcacaaccct tcgaaggttt
    53881 aggctccggt gtcatcatca acgccaataa aggctatgtg ctgaccaaca accatgtgat
    53941 taatcaggca cagaaaatca gtattcagct caatgatggg cgcgagtttg atgcaaaact
    54001 gattggtagc gatgaccaga gcgatatcgc cctgttgcaa attcaaaacc cgagcaaatt
    54061 aacgcaaatc gctattgccg actccgataa attgcgcgtc ggtgattttg ccgtggcggt
    54121 cggtaaccca tttggccttg ggcaaaccgc cacctctggc attatatccg cattaggccg
    54181 cagcgggttg aatcttgaag gtctggaaaa ctttatccag acagatgctt ccattaaccg
    54241 cggtaactcc ggcggtgcac tgttaaacct taacggtgag ttaattggca tcaacactgc
    54301 aatccttgcg cctggcggcg gtagcgtcgg gattggattt gccattccca gtaatatggc
    54361 gcgaacactg gcgcagcagc ttatcgactt tggagaaatc aaacgcggcc tgttaggcat
    54421 caaaggcacc gagatgagtg ccgatatcgc caaggccttc aaccttgacg tgcagcgtgg
    54481 cgcgtttgtc agcgaagtgt tgccaggttc tggctcggcg aaagcgggcg tcaaagcggg
    54541 cgatattatt accagcctca acggcaaacc gctgaatagc tttgctgagt tgcgctctcg
    54601 tatcgcgacc accgagccgg gcacgaaagt gaagcttggc ctgctgcgta acggcaaacc
    54661 actggaagta gaagtgacgc tcgataccag cacctcttcg tcggccagcg ctgaaatgat
    54721 cacgccagcg ctggaaggtg caacgttgag cgatggtcag ctaaaagatg gtggcaaagg
    54781 tattaagatc gatgaggttg tcaaaggaag cccagctgct caggctggct tgcaaaaaga
    54841 cgatgtgatc attggcgtca accgcgatcg ggtgaactcg attgctgaaa tgcgtaaagt
    54901 gctggcggca aaaccggcca tcattgccct gcaaattgta cgcggcaatg aaagcctcta
    54961 cctactaatg cgttaatgtc gtaaaccggg catcaggctg acgtgtgatg tccggttaac
    55021 tcgtggtatg ctgctgccgt tccctttttt aacgatgcct ccatcatgtt tgtgaagctc
    55081 ttacgttccg ttgcgattgg attaattgtc ggcgctattc tgctggttgc catgccttcg
    55141 ctgcgcagcc ttaacccgct ttccactccg caatttgaca gtaccgatga gacgcctgcc
    55201 agctataatc tggcggttcg ccgcgccgcg ccagcggtgg ttaacgttta taaccgcggt
    55261 ttgaacacca actctcacaa ccagcttgag atccgcaccc tgggatctgg tgtaatcatg
    55321 gatcaacgcg ggtatatcat caccaataaa cacgtgatca acgacgccga tcagatcatc
    55381 gtcgccttac aggatggacg tgtttttgaa gcgttactgg ttggctctga ctctctaacc
    55441 gatctggcgg tgctgaaaat taacgccaca ggcggtttac caacgattcc gatcaatcca
    55501 cgccgcgtac cgcacattgg cgatgtggtt ctggcgatcg gtaacccgta caacctcggg
    55561 cagaccatta cccaagggat tatcagtgcc acgggtagaa tcggtctgaa cccgaccggg
    55621 cggcaaaact tcctgcaaac cgatgcttcc attaaccacg gtaactctgg cggcgcgctg
    55681 gtgaactcgc tgggcgaact gatgggcatt aacacgctgt cgtttgataa gagtaacgat
    55741 ggcgaaacgc cggaaggtat cggctttgcg attcctttcc agttagcgac caaaattatg
    55801 gataaactga tccgtgatgg ccgtgtgatc cgtggctaca ttggcattgg tggacgtgag
    55861 atcgccccat tacatgcgca gagtggcggc atggatcaac tgcaagggat cgtagttaac
    55921 gaagtgtcgc ctgatggccc agcggcaaat gcaggcatcc aggtcaacga tctgattatc
    55981 tctgtgaata acaaaccggc catctctgct ctggagacga tggatcaggt ggcagaaatt
    56041 cgccctggtt cggtgatccc ggttgtcgtg atgcgtgacg ataaacagtt aacattgcag
    56101 gtcaccattc aggaatatcc ggcgaccaac taaggtcagc gtaaaaaaac aaaaaccgga
    56161 gtctgtgctc cggtttttta ttatccgcta atcaattact tattaacgaa ctcttcgccc
    56221 agggcgatat ctttcttcag cgtatccagc ataccttcca gcgcgttctg ttcaaatgcg
    56281 ctcagagtac cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga
    56341 gagaagaaac gagcgtactg accgtcgcct tcaacgtagg cacattcgac aacgccttgt
    56401 tcgccctgca gtgcgcgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac
    56461 agggttgcag acccgccacc ggctttcgct tcaaccacct cagtacccgc gttctggata
    56521 cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc
    56581 agcggcagaa tggtaacacc agagtgacca ccaataaccg gcacttcaac ttcgcctggc
    56641 tgtttgcctt tcagttccgc aacaaaggtg ttggaacgaa tgatatccag cgtggtaacg
    56701 ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca
    56761 actgtagtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca
    56821 acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc
    56881 atacccggtt tacgcgctac gcctgcagag ataagaacga catctgcacc ttccagcgcc
    56941 ggagtcgcat cttcacctga aaaacctttg attttcacag cagtagggat atggctcaga
    57001 tcgacagcca caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa
    57061 ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg
    57121 actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctgcgct
    57181 gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt
    57241 cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt
    57301 aacagtcact tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgct
    57361 cagccaaaac aatatcaatt tgataacaat taatttactt ttaagcagaa tttgcaggcc
    57421 gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt
    57481 atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg
    57541 ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt
    57601 taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc
    57661 cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac
    57721 caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc
    57781 tgaactgggt gtaccaacca cctccagtcc attgaagaat ctggtactgg atatcgacta
    57841 caacgatgca gttgtcgtga ttcataccag ccctggtgcg gcgcagttaa ttgctcgcct
    57901 gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat
    57961 ctttactacc cctgctaacg gtttcaccgt caaagagctg tacgaagcga ttttagagct
    58021 gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt
    58081 ttgcctatca accccctgct ttcccctgcg attcatttaa cgaatagtg
//
LOCUS       DADTFG010000009        57755 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.009, whole genome shotgun sequence.
ACCESSION   DADTFG010000009 DADTFG010000000
VERSION     DADTFG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.009"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            46..1812
                     /gene="adeD"
                     /locus_tag="J1138_000591"
     CDS             46..1812
                     /gene="adeD"
                     /locus_tag="J1138_000591"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBA8452434.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1859..3250)
                     /gene="uhpT"
                     /locus_tag="J1138_000592"
     CDS             complement(1859..3250)
                     /gene="uhpT"
                     /locus_tag="J1138_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBA8452435.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3388..4707)
                     /gene="uhpC"
                     /locus_tag="J1138_000593"
     CDS             complement(3388..4707)
                     /gene="uhpC"
                     /locus_tag="J1138_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBA8452436.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4717..6219)
                     /gene="uhpB"
                     /locus_tag="J1138_000594"
     CDS             complement(4717..6219)
                     /gene="uhpB"
                     /locus_tag="J1138_000594"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBA8452437.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAFTLQGWQQDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6219..6809)
                     /gene="uhpA"
                     /locus_tag="J1138_000595"
     CDS             complement(6219..6809)
                     /gene="uhpA"
                     /locus_tag="J1138_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBA8452438.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6882..7172)
                     /gene="ilvN"
                     /locus_tag="J1138_000596"
     CDS             complement(6882..7172)
                     /gene="ilvN"
                     /locus_tag="J1138_000596"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBA8452439.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7176..8864)
                     /gene="ilvB"
                     /locus_tag="J1138_000597"
     CDS             complement(7176..8864)
                     /gene="ilvB"
                     /locus_tag="J1138_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBA8452440.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8970..9068)
                     /gene="ivbL"
                     /locus_tag="J1138_000598"
     CDS             complement(8970..9068)
                     /gene="ivbL"
                     /locus_tag="J1138_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBA8452441.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9469..10653
                     /gene="emrD"
                     /locus_tag="J1138_000599"
     CDS             9469..10653
                     /gene="emrD"
                     /locus_tag="J1138_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBA8452442.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(10661..11158)
                     /locus_tag="J1138_000600"
     CDS             complement(10661..11158)
                     /locus_tag="J1138_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBA8452443.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11155..11517)
                     /locus_tag="J1138_000601"
     CDS             complement(11155..11517)
                     /locus_tag="J1138_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBA8452444.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11507..11854)
                     /locus_tag="J1138_000602"
     CDS             complement(11507..11854)
                     /locus_tag="J1138_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBA8452445.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(11914..13407)
                     /locus_tag="J1138_000603"
     CDS             complement(11914..13407)
                     /locus_tag="J1138_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBA8452446.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPISHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDRFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDLNEMHNLIDDIRFADVRRKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(13404..15119)
                     /locus_tag="J1138_000604"
     CDS             complement(13404..15119)
                     /locus_tag="J1138_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBA8452447.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPLFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAVVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLMIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            15286..16152
                     /locus_tag="J1138_000605"
     CDS             15286..16152
                     /locus_tag="J1138_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA8452448.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHANLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(16242..17903)
                     /locus_tag="J1138_000606"
     CDS             complement(16242..17903)
                     /locus_tag="J1138_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBA8452449.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(18100..18528)
                     /gene="ibpB"
                     /locus_tag="J1138_000607"
     CDS             complement(18100..18528)
                     /gene="ibpB"
                     /locus_tag="J1138_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBA8452450.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(18640..19053)
                     /gene="ibpA"
                     /locus_tag="J1138_000608"
     CDS             complement(18640..19053)
                     /gene="ibpA"
                     /locus_tag="J1138_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBA8452451.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            19359..19691
                     /locus_tag="J1138_000609"
     CDS             19359..19691
                     /locus_tag="J1138_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBA8452452.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     gene            complement(19695..20942)
                     /locus_tag="J1138_000610"
     CDS             complement(19695..20942)
                     /locus_tag="J1138_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBA8452453.1"
                     /translation="MAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPS
                     GASFTGETIERVNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYY
                     DFHHRRGVIVESGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMH
                     ELDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYE
                     EGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPR
                     GVVQRRLTFTHHRIYPGLVNIPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEP
                     RQLTHNKTDIQSAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVV
                     FSPDGQWLAWMEDGQLWITETDR"
     gene            21010..22110
                     /gene="cbrA"
                     /locus_tag="J1138_000611"
     CDS             21010..22110
                     /gene="cbrA"
                     /locus_tag="J1138_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBA8452454.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTALKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKLPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(22071..23408)
                     /locus_tag="J1138_000612"
     CDS             complement(22071..23408)
                     /locus_tag="J1138_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452455.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(23483..24631)
                     /gene="dgoD"
                     /locus_tag="J1138_000613"
     CDS             complement(23483..24631)
                     /gene="dgoD"
                     /locus_tag="J1138_000613"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBA8452456.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(24628..25245)
                     /locus_tag="J1138_000614"
     CDS             complement(24628..25245)
                     /locus_tag="J1138_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBA8452457.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(25229..26107)
                     /locus_tag="J1138_000615"
     CDS             complement(25229..26107)
                     /locus_tag="J1138_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBA8452458.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSAGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(26104..26793)
                     /gene="dgoR"
                     /locus_tag="J1138_000616"
     CDS             complement(26104..26793)
                     /gene="dgoR"
                     /locus_tag="J1138_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBA8452459.1"
                     /translation="MTLNKTDRIVITLGRQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            27072..27728
                     /locus_tag="J1138_000617"
     CDS             27072..27728
                     /locus_tag="J1138_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452460.1"
                     /translation="MKLNLKGFFKAASLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPHGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(27772..28584)
                     /gene="yidA"
                     /locus_tag="J1138_000618"
     CDS             complement(27772..28584)
                     /gene="yidA"
                     /locus_tag="J1138_000618"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBA8452461.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(28699..29106)
                     /locus_tag="J1138_000619"
     CDS             complement(28699..29106)
                     /locus_tag="J1138_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBA8452462.1"
                     /translation="MIIMGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(29337..31751)
                     /gene="gyrB"
                     /locus_tag="J1138_000620"
     CDS             complement(29337..31751)
                     /gene="gyrB"
                     /locus_tag="J1138_000620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBA8452463.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(31780..32853)
                     /gene="recF"
                     /locus_tag="J1138_000621"
     CDS             complement(31780..32853)
                     /gene="recF"
                     /locus_tag="J1138_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBA8452464.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(32853..33953)
                     /gene="dnaN"
                     /locus_tag="J1138_000622"
     CDS             complement(32853..33953)
                     /gene="dnaN"
                     /locus_tag="J1138_000622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBA8452465.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(33958..35361)
                     /gene="dnaA"
                     /locus_tag="J1138_000623"
     CDS             complement(33958..35361)
                     /gene="dnaA"
                     /locus_tag="J1138_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBA8452466.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            35658..35738
                     /locus_tag="J1138_000624"
     CDS             35658..35738
                     /locus_tag="J1138_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452467.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            35968..36108
                     /gene="rpmH"
                     /locus_tag="J1138_000625"
     CDS             35968..36108
                     /gene="rpmH"
                     /locus_tag="J1138_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBA8452468.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            36125..36484
                     /gene="rnpA"
                     /locus_tag="J1138_000626"
     CDS             36125..36484
                     /gene="rnpA"
                     /locus_tag="J1138_000626"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBA8452469.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            36448..36705
                     /gene="yidD"
                     /locus_tag="J1138_000627"
     CDS             36448..36705
                     /gene="yidD"
                     /locus_tag="J1138_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBA8452470.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            36708..38354
                     /gene="yidC"
                     /locus_tag="J1138_000628"
     CDS             36708..38354
                     /gene="yidC"
                     /locus_tag="J1138_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBA8452471.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            38460..39824
                     /gene="mnmE"
                     /locus_tag="J1138_000629"
     CDS             38460..39824
                     /gene="mnmE"
                     /locus_tag="J1138_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBA8452472.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDSDGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNNVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            40066..40140
                     /gene="tnaC"
                     /locus_tag="J1138_000630"
     CDS             40066..40140
                     /gene="tnaC"
                     /locus_tag="J1138_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBA8452473.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            40361..41776
                     /gene="tnaA"
                     /locus_tag="J1138_000631"
     CDS             40361..41776
                     /gene="tnaA"
                     /locus_tag="J1138_000631"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBA8452474.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            41868..43115
                     /gene="tnaB"
                     /locus_tag="J1138_000632"
     CDS             41868..43115
                     /gene="tnaB"
                     /locus_tag="J1138_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBA8452475.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            43247..44422
                     /gene="mdtL"
                     /locus_tag="J1138_000633"
     CDS             43247..44422
                     /gene="mdtL"
                     /locus_tag="J1138_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBA8452476.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            44397..45356
                     /gene="yidZ"
                     /locus_tag="J1138_000634"
     CDS             44397..45356
                     /gene="yidZ"
                     /locus_tag="J1138_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBA8452477.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMSVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            45513..46262
                     /locus_tag="J1138_000635"
     CDS             45513..46262
                     /locus_tag="J1138_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452478.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            46284..46850
                     /gene="yieF"
                     /locus_tag="J1138_000636"
     CDS             46284..46850
                     /gene="yieF"
                     /locus_tag="J1138_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HBA8452479.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQSTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(46904..48241)
                     /locus_tag="J1138_000637"
     CDS             complement(46904..48241)
                     /locus_tag="J1138_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA8452480.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            48408..49073
                     /gene="yieH"
                     /locus_tag="J1138_000638"
     CDS             48408..49073
                     /gene="yieH"
                     /locus_tag="J1138_000638"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBA8452481.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            49140..49613
                     /locus_tag="J1138_000639"
     CDS             49140..49613
                     /locus_tag="J1138_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBA8452482.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMIACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(49695..50417)
                     /locus_tag="J1138_000640"
     CDS             complement(49695..50417)
                     /locus_tag="J1138_000640"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBA8452483.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(50432..51601)
                     /locus_tag="J1138_000641"
     CDS             complement(50432..51601)
                     /locus_tag="J1138_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBA8452484.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNELAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(51628..53244)
                     /locus_tag="J1138_000642"
     CDS             complement(51628..53244)
                     /locus_tag="J1138_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBA8452485.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(53330..54724)
                     /locus_tag="J1138_000643"
     CDS             complement(53330..54724)
                     /locus_tag="J1138_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA8452486.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYSLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(54743..56617)
                     /gene="bglF"
                     /locus_tag="J1138_000644"
     CDS             complement(54743..56617)
                     /gene="bglF"
                     /locus_tag="J1138_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBA8452487.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKVQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGQIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(56751..57587)
                     /gene="bglG"
                     /locus_tag="J1138_000645"
     CDS             complement(56751..57587)
                     /gene="bglG"
                     /locus_tag="J1138_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBA8452488.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT        14242 a        15344 c        14341 g        13828 t
ORIGIN      
        1 tatgataaat aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattctatt
       61 aaccataaat ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt
      121 ggagacgctg ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatggc
      181 ggagaaattt ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa
      241 tacgctgatg ctccggcttt gcagcggatt gatgcccgcg gcgcaacggc ggtgccaggg
      301 tttattgatg ctcacctgca tattgaatcc agcatgatga cgccggtcac ctttgaaact
      361 gctaccctgc cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg
      421 atgggagaag ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag
      481 tacttacagg tcagctcttg cgtacccgcc ctggaaggct gcgatgttaa tggtgccagt
      541 tttacccttg aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa
      601 atgatggact accctggcgt aattagcggg cagaatgcgc tgctcgataa actggacgca
      661 tttcgccacc tgacactgga cggtcactgc ccgggtttgg gtggtaaaga acttaacgcc
      721 tatattgctg cgggtattga aaactgccac gaaagttatc agttggaaga aggacgccgg
      781 aaattgcaac tcggcatgtc attgatgatc cgtgaagggt ccgcagcccg caatctcaac
      841 gcgctggcac cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac
      901 cgtaacccgt gggagatcgc ccatgaagga cacatcgatg ccttaattcg ccgcctgatc
      961 gaacaacaca atgtaccgct gcatgtggca tatcgcgtag ccagctggtc gacggcgcgc
     1021 cattttggtc tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg
     1081 ttgagcgatg cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga gccgatcgat
     1141 gcacaaacct tacaggcgga agagtcggcg agactggcac aatccgcccc gccatatggc
     1201 aataccattg atcgccagcc agtttccgcc agcgactttg ccctgcaatt tacccccgga
     1261 aaacgctatc gcgttattga ggtaatccat aacgaactga tcacgcactc ccgctccagc
     1321 gtctacagcg aaaacggttt tgatcgcgat gatgtgtgct ttattgccgt tcttgagcgt
     1381 tacggacaac gactggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggt
     1441 gcgctggctg cgacggtcag ccatgacagc cataatattg tagtgatcgg tcgtagcgca
     1501 gaagagatgg cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggtc
     1561 cgtaacggcc aggtacaaag tcatctaccg ttgcccattg ccgggctaat gagcaccgac
     1621 acggcgcagt cactggcgga gcaaattgac gccctgaaag ccgccgcccg tgaatgcggt
     1681 ccgttaccgg atgagccgtt tattcagatg gcgtttcttt ccctgccagt gatccccgcg
     1741 ctgaaactaa ccagtcaggg gctgtttgat ggtgagaagt ttgccttcac tacgctggaa
     1801 gtcacggaat aataaaaaaa agcccggcgt catgccgggc aaaagtcacc agttacgttt
     1861 atgccactgt caactgctga atttttttct cgcggcggat tttgcgttct tccatcaccg
     1921 ccactatcgc catcaggcag atacaaccaa tcgcggcgat atccagcgcg gcgaaggtgc
     1981 ctgcccagcc ggtaaggcca aataccggcg tcccatcggc aatcattccc agacctaact
     2041 tggcaaagct gtcaccaatc aggtaagcga aggtgccttt aataccatcg gcagcgccaa
     2101 tcgctttttt aggtacaaag ccaacagccg ccacaccaat caacaattgc gggccaaaga
     2161 ccaggaaacc caacgcaaag agagaagcca ggtagatata ttcgttgctg gcgtgttgat
     2221 acacaccaag cgtggcgata atcagcgcca gcgcgataca ggccaccagg ccacggcgac
     2281 cgttcgccag gtcagagagc cagccccaca gcagcgtacc gaccagcgca ccagcttcaa
     2341 acagcgtaaa gccctgaatt gccaccgctt tagagagttt cagttcctgg aacgcataca
     2401 cggttgacca ctggtcgata ccaatacgca ccacatagag gaaaatgttg gcaaagcaca
     2461 gcagccagat cactttgttt ttcagcacat actcaacaaa gatctgccac ttggtcatat
     2521 cggtagattc tgtctcttta tcctcttcac tgatctcctc gccaaacagc tcttcagctt
     2581 tgccgaggcc ataagattcc ggggagtcgc tgccgtaacg cagaccgata aaaccaacaa
     2641 tcagcgcgat aatcgacggg aaaataaaca tgccgatgac atgaccatcg aacaggtaat
     2701 ttgcgccgaa cagcgccaca cctgctgcgc ctgcaccacc aaggttgtga gaaatgttcc
     2761 agaaaccgag gaatgtcccg cgtttacgac gcggcgtcca tttggtgatg gtggagtaac
     2821 tgcacgaacc gccggtactc tggaaaaagc cgctcaaggc gtagaaggca atcatcagga
     2881 acaggctaac cgagccgctg cccatactgg cactgaagcc cagcatacaa atggcagaca
     2941 ggatcagcat gaacggcagg aattgtttgg tgtttttgcc gtcggcgtag taagaaacca
     3001 gcgttttacc cacgccataa gtgatggaga agcccaggcc gatcatcccc agctgcgtca
     3061 tgctcaaccc gtaggtcgag atcatgtcgt tctgcgcgat gttaaagttc ttgcgaatca
     3121 ggtacatcgt aaggtagccg ataaagacca ccaggtagga ttgcatgaac ggtttgaacc
     3181 acattttgcg ccgcacttcg agcggaaggt ccagggtcgg cttgcgaacc tggtttaaga
     3241 aagccagcat gggttactcc tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca
     3301 gcagcaaatc aggcctgaga tagcgtccag gcaaaacctg gaaaatttct tagttttgcc
     3361 ggatatgaag tgaaaaggtg agatgcatca cgcttctcgc ggtgtctggg cgttcaaaaa
     3421 gggcagtaat agcagtgcgg aaatcccggc ggcgatggcg ataaccacaa aaaatccgct
     3481 ccagtgccag gtatcgagta ctttcgccag cggccaacca gcaagcgacg caccaagata
     3541 agcaaacaag ccgacaaatc ccgtcgccgc ccctgccgcc tctttgtggg aacactctgc
     3601 cgccgccatg ccgattaaca tctgcgggcc aaagacaaaa aaaccaatgg tgaagaagca
     3661 ggttgcctgc atcacgtagc tggcaaatgg catcagccac agcgagccga ctgaaagcaa
     3721 aattccggcg gcgaaaatca aattcatcgg cccgcggttg ccgttaaaca gtttgtccga
     3781 accccaaccc gctaccagcg caccaataaa tccgcccagt tcaaacattg tcactgccgt
     3841 attcgccgtg accagatcga cgcccagagt ctcggacata tacaaattgc cccagtcgtt
     3901 gatcgccgcc cgaaccacat agaccagcac atagcaaaac gaaagcagcc agatatacgg
     3961 attcagcaac acatatttgg tgaggatctc tttacgcgtc agccctgccc cttcttgttg
     4021 ttgagcaatc tccagtgcgt cgtgccgcca ttcaccaacc gccggtaaac ctaacgcctg
     4081 cgggcgatcg cgcagccgcc agcagagaaa aatccccacg actatcgcca tacaacccgc
     4141 aatcatcatc ccggcacgcc agccgtaatg cagcgcgctc gctgccatca caatgggaat
     4201 gagtgcgccg ccgacgttat gcgccgtgtt ccataatgcc caccaaccgc cgcgctcggt
     4261 acgagaatac caggcggtta acagacgcgc acacaccggt gaaccccagc cctggaaaaa
     4321 ggcgttcaac acccaaagca cggcaaacgc ccatagcgac gtcgagaagc caaacagaat
     4381 gttgataatg cccgtggcaa taagccctat ccccataaaa taacgggcat ttgagcgatc
     4441 gctgacaatg ccggagacaa acttcgacac accgtaggta atgtaaaaca gggtcgctaa
     4501 caggccaata tcgctacggc tgagcacgcc gttagcaagg atttctggta cggcggcgtt
     4561 aaaactttta cgcgtgaagt aaaacagcgc gtaacccagc cagatggtca gcagaatatg
     4621 ccgacgccag tagcgatagc gggcatcaat ttcatgttta tcagtcatta atggcgcatc
     4681 ggcaggcgct ttcagaaacg gcaacatcat cgaaccttag acataacgtt gaggtagaga
     4741 aacgctgaca cgcgtgccgt gcagacagga aatggtcaat gtgccaccca gcgccgttac
     4801 gcgctcgcgc attccggtaa ggccaaaacc gtgttgcccg gaatctggcg gtaaaccgct
     4861 gccatcgtct tcaatcacca gcatcaaccg ttcatcctgc tgccagcctt gcagggtgaa
     4921 cgcgctggca tcagcatgtt tcacaatgtt gttcagccct tcctggcaga cacgaaacag
     4981 cgtcactcgc tggttttcgc ttaacgctga ttcatcgatt cgccattcga gatggctgac
     5041 aatgccgcgc ccttccagct ccatttcccg catcagtgag cggatggcct gctccagggt
     5101 gagatcatcc aactggcgcg gacgtaaccg acctaacaaa cggcgcaccg cgtcgtaaac
     5161 gcccagcgat agttgttcga tgagctgccc gctctgtttc acgctggcgt tatctgccgc
     5221 cagccgctga acaatgcccg cttgagttcg aatagcagta atggtctgac cgatatcatc
     5281 gtgcagctca cgtgccacat cacggcgcac gctctcttcg gtttccagca accgttcagc
     5341 gagatgctga ttgcgcgcca gttccttttg cagcgactgg ttaagttcac gcaaccgctg
     5401 gatgcctgcg cccagtaaca accctgtcag actttgcacc aacagcgaga gcaataaatc
     5461 caccggatga tcgcgccagg tttgactggc gatcagcgcg atggcgttca tcaatgtcgc
     5521 aatcagcgct ccttgccaac catagtgcca ggccagcgcg ataatcggca gcgccagaca
     5581 gaatggcgta aagcgcgaca gttcgtctgg caatcccaac tggagccaga gactgataac
     5641 aaacagcagc aagtaccaga ccagatgtcg cccgcgccag ttgattggct gagaaaccag
     5701 tgacgggccg agcggcagcc aggtgttatt cgccagatag tgccagaaca ccagacatat
     5761 cggggccagc gtcaggccgc cagttaaagt cagcagcagc gcattccacg actctttgcc
     5821 gtgccaaagc cagggcagcg actgcaacaa cgccgccgca gttaacgccg caccctgtaa
     5881 cagcaaggtg cgccagtcac gttgatggcg atagcgcgag atcagcgcca cgggcaataa
     5941 cgtcagtaaa ctaccgatca tcaataagga gaaatgggtt aaaccgaccg cctgcattag
     6001 ccagtaaatc agcagccact ccgcacccag tagcaccggc cagtatccgc gcgggcattg
     6061 cagcattagc cctagccgca ggccaaacgg aaataacagc accgccatat cagggcgctc
     6121 aaccaggtgc aggctgatac tccacaggca aaaccatgcg gcagagaaga taaaaaagca
     6181 ggcaataacg gtaattaagc gggagaacaa cgtcttcatc accagccatc aaacatgcgg
     6241 cgcgccagtt caacgtcgtt actgacgccc agtttttcca tcagattggc gcgatggacg
     6301 tgtaccgttt tcggcgacaa gcccagttcg gcggcaatct ctttcaccgc cattccttgc
     6361 gccagttttt ctgccacctg gcgttcgcgt ttggttagcg ggtcctggcg accggatgcc
     6421 agtttaatgg caatatccgg cgtcagataa cagccgccag tggcaaccgt atgcaccgca
     6481 gcaatgagtt catccgggct acagcgtttg gagagaaagc cgcgcgcccc ggcgttaagc
     6541 gcctgctcaa ccagcgccgg actgtcgtga acggagagca taattgtcgc catgcctttc
     6601 ggcagctggc ttagaagctc cagaccggag atatcaggca tggagatatc gcaaatacac
     6661 acctgcacac cgcgccccgg cagccccgcc agcgcctcgc gccccgaacc aaactcggca
     6721 actacctgca aatcaggttc cagccccagc agctgcgcaa agccggagcg gacgataagg
     6781 tgatcgtcta taagggcaac ggtgatcatg atcttgtcct ggagggtaat aaaacgcgct
     6841 taccttaacg ataagcgcga tgttgttcaa gccttgagcg gttactgaaa aaacaccgca
     6901 atcttgttaa acatggtcgg atcggactga ttacgctgca ctttcaccac atcttccagc
     6961 ttgtcgattt ggcttatcat ctgctccaga cgctggtcgt cattgaccag tagccagatg
     7021 tggcttttgt cgctgtcctg aatcggcagg cagagaatgc cttcaacgtt aaaagcgcga
     7081 cgggcaaaaa ggccacaaac gtgggtcatt acacccggat ggttgcgaac ggtgagttcc
     7141 agaatcacgt tatcatgagt tgtgttttgc atggcttatt cccccaccat ttcagtattc
     7201 gccgcacctg gcggcaccat cgggtacact ttttcttcgg catcaatgcg cacatggatc
     7261 agcgccgggc cagggcgatt gatgatttcc tgcaatgcag cctgcggatc ggcttcgtta
     7321 ttcaaatcac aggtttcgag gccgaatccg gcggcaatct gcataaagtt gattttgccc
     7381 ggataggtag cggcaaaaac gccctgcttg tagaacagac tctgttgctg atgcaccagc
     7441 cccagcgctt cgttgttcat caaaatgatt ttaacatcca gctgattttc actggcggtc
     7501 gccatctcct gaatattcat catcaggctg ccgtcgccgg agaagcacaa cactttgcgg
     7561 tccgggttcg ccagcgccgc gccaatcgcc gcaggcaggc caaaacccat cgtgcccagc
     7621 ccgccggagg tcagccactg gcgtgggcga ttgagcggat aggcttgcgc ggtccacatc
     7681 tgatgctgac ccacgtcggt ggtgataatc gcattgtcat cgacacaggc ggcaacggcg
     7741 ttgatcaggc cgtaatggct taatggatcg cacgctttcg ggattggaca cgggaattca
     7801 cgctgcaaat ccgccaccaa ctggtgccac tctgcacgcg gttgcgcttc caccagcggg
     7861 atcaactgcg ccaacacgtc atcaacatcc gcctgaattg ccacatgcgg ttgcttgatt
     7921 ttgcccagct ctgcgcggtc gatatcgaca tgaataattt tggcattcgg acagaactgc
     7981 tcggttttgc caatcgcccg gtcatcaaaa cgcgcaccga gtacaatcag cagatccgcc
     8041 tcctgcaaaa tatagttggt gctgcgcaca ccgtgcatcc ccagcatacc cagcgacagc
     8101 ggatgcgctt ttggcaacat acccagcgcc attaaagtca tggtggtagg cagttgcgct
     8161 ttctccgcca gttcacgcac tcgcgcaggc gcgttgatca caccaccacc cagataaagc
     8221 accgggcgtt tggcggcgtt aatcatcgcc gccgcgtcac gaatactttc ttcgctaaag
     8281 gcgggtgcgg cggctttttc tgccatagcg ggctgtgttt caatctcaaa aactgccgtt
     8341 tgcacatcct taggaatgtc tatccacacc gggccagggc ggcctgattg cgcaatacgg
     8401 aaggcatcgc tcatgacctg cgggagttct tcgatatgtc tgaccagata gttgtgtttg
     8461 gtgatgggga tagagatgcc gtaggtgtcg acttcctgga aggcgtcggt gccgatcatc
     8521 gaggcgggaa cctgaccagt gatgcaaatc agcgggatgg agtccagcct cgcatcggca
     8581 atggcggtca ccaggttagt cgcacccggt ccgctacagg ccatacagac cgccggttta
     8641 ccgtcggtgc gcgccattcc ctgagcgata aaccccgcgc cctgttcatg gcgagccaga
     8701 atatggcgga tttgcgtgct ttggcttaag gcatcgtaaa caggcaggat agaaccgccc
     8761 ggaatacccg tcacaatctt aatgccctgc tgttccagga aatgaacgat aaattctgcg
     8821 ccggtaaagc gcttacgcgt cgatgttgtg cccgaacttg ccatgctcca gtccttttct
     8881 tctgggccga ctttccggga ggagcttaaa cgaaaaaccc cgcccggttt gcgccggcgg
     8941 ggttttggaa tcgtgtgttg ttccagtccc tacggcgcat tgccgacgac caccaccaca
     9001 cgcacgacga ccactgcggc ggatggcgca gttggtagta gttttgcgtt gaacatggaa
     9061 gtagtcatta gggaccttgt tggttttgtg tttaacaata tttatacaag cacagcttta
     9121 caggggagac aatggaaaat ttttcttccg gataaaaata cgtggcagat cacattttga
     9181 actgaaactc aggcaataaa gcatcatcac ctgaaagacg gtcagattgt tgacagagtg
     9241 cgccttattc atgtcgaata cagcgtaaac accttaccct accaaccgca ccatcactgt
     9301 tcccggcaac atttattgcc gcttttgttt acatattctg ccgctaaaca attccccatt
     9361 cctggcgtat atctggctaa cattcatcaa tgtgatagat tcctctcccg catttatggg
     9421 aatgcgtagt gacttattct aattattttt ataaaagcat ccgtgataat gaaaaggcat
     9481 agaaacgtca atttgttatt gatgttggta ttactcgtgg ccgtcggtca gatggcgcaa
     9541 accatttata ttccagctat tgccgatatg gcgcgcgatc tcaatgtccg tgaaggggcg
     9601 gtgcagagcg taatgggcgc ttatctgctg acttacggtg tctcacagct gttttatggc
     9661 ccgatttccg accgcgtggg tcgccgaccg gtgatcctcg tcggaatgtc catttttatg
     9721 ctggcaacgc tggtcgcggt cacgacctcc agtttgacgg tattgattgc cgccagcgcg
     9781 atgcagggga tgggcaccgg cgttggcggc gtaatggcgc gtactttgcc gcgcgattta
     9841 tatgaacgga cacagttgcg ccatgctaac agcctgttaa acatggggat tcttgtcagt
     9901 ccgttgctcg caccgctaat cggcggtctg ctggatacga tgtggaactg gcgtgcctgt
     9961 tatctctttt tgttggttct ttgtgccggt gtgactttca gtatggcccg ctggatgccg
    10021 gaaacgcgtc cggtcgacgc accgcgcacg cgcctgctta ccagttataa aacgcttttc
    10081 ggtaacagcg gttttaactg ttatttgctg atgctgattg gcggtctggc cggaattgcc
    10141 gcctttgaag cctgctccgg cgtgctgatg ggcgcggtgt tagggctgag cagtatgacg
    10201 gtcagtattt tgtttattct gccgattccg gcggcatttt ttggcgcatg gtttgccgga
    10261 cgtcccaata aacgcttctc aacgttgatg tggcagtcgg ttatctgttg cctgctggct
    10321 ggcttgctga tgtggatccc cgactggttt ggcgtgatga atgtctggac gctgctcatt
    10381 cccgccgcgc tgttcttttt cggtgccggg atgctgtttc cactggctac cagcggcgcg
    10441 atggagccgt tccccttcct ggcgggcacc gctggcgcgc tggtcggcgg tctgcaaaac
    10501 attggttccg gcgtgctggc gtcgctctct gcgatgttgc cgcaaaccgg tcagggcagc
    10561 ctggggttgt tgatgacctt aatgggattg ttgatcgtgc tgtgctggct accgctggcg
    10621 acgcggatgt cgcatcaggg gcagcctgtt taagctcacg tcaccgcagc atcgtcatca
    10681 gctccatggg agaacgatgc tgctttatca gatcacgcat cacccgcata tgcggcgcgg
    10741 agtaagaata aaacgcctga tagcccgcac aaagcacgtt gtctggcgtc tcctcccgag
    10801 cgtgcgggca caggcgatgc cagtcgcacg cctggcagcc acgacgcggt tgcgggtttt
    10861 cgctgccaca ccatttttgt agggtggcgt caaaaagctc aatcgaaata cgactgatat
    10921 ctgcccgtac ccaacactcg aacagggcaa ttaaaaattt cccccacagc tctgctgtca
    10981 ccgaagcggc cgtcagatcg ccccggtcat caaattcaag atggggaata agctgtacgg
    11041 agcggatacc ccgttgtttg aaattgtgat aaaaatcggc aggttgcaga gcatccttgt
    11101 actctacaac cagtttatgc ctgtcctctt cagcatcaat gacctgactt cctgtcatgc
    11161 gactctctca ataaatacga taagttgatg tatatgcgtt acagcaaaca gtattgcgag
    11221 agataacacc gcgagggaga tcaaaaattt gtcacggact acgtgaaatt gggaaaaatc
    11281 gctattcgtg acatccatta aattgcgatt acgggtgtag tgccagagga tcagcgccac
    11341 aatggcgaga atgccaatcg aaatccagaa taacatgccc gcctggtgcc agttgtgttt
    11401 gatagccaac gccatcaacg cgccgtagcc cagcatggta cgaaaccacg ccagtgatgt
    11461 acgctccggc tgcagccctg ggtcggcaat gcgtctggct ttgcggctat ccggcataca
    11521 acaccagtcc cataacgatc accgctacga ccatcaatat taagctgatg attaataagc
    11581 tgttggtgta gggcaggtcc tctttcagac gcatcgcttt ttcattgcgt aaccagcgca
    11641 ggtagccata catcgctaac ccgccggaaa acagacacaa caacaacgcc agcagttcgc
    11701 gaatgacggg cgtggcgaaa tctggcgcaa gttgatcaag cccaaccccc gccgccagaa
    11761 agcctagtgc ggtacggatc cacgccagaa aagtacgctc attagccagc gagaagcgat
    11821 aatcaggtgc ttctccgagg cgggaaattt tcattgttgc tccttgtaaa ctgttcctcg
    11881 ccgaaaaagt aggccaggaa cagcaataat gcatcagaac ttctgttttt tctcttccac
    11941 cttcacccct tgtgtcggta ggccggtgtc atagtcgcgt accactggcg aatagccatc
    12001 ttgtgggcgt ggacgaaacg cccccatcca gcgcggtcgt gcatctttac gccacggacg
    12061 cagactccac tggtaactgc ggaacggatc gcgaatttta tccatgtaat ccaataaggc
    12121 gtcatgcatt ttgcggcgaa cgtcggcaaa acggatatca tcgatcagat tatgcatttc
    12181 atttaggtca ttacggcgat cgtaaagttc atcactggtg aagaggttga gtaccagttt
    12241 aaagtcatcc gtcacccagc aacgcaccgg aataaaaccg ccaaatctgt catgctcaat
    12301 ctcgtagcgg ttaaattcca ccattacgcc gcgtggctct ttcacggcaa ggatattttc
    12361 ccccggcaga atctctggtt tttcaatatc tgccagcgcc atcattgtcg gcagtaaatc
    12421 gatatgactg attggcgtat cgacctgccg ccgctcccct tgcggcgaac ggatgatcag
    12481 cggaatacgg gtgatgtcgt catacatcgc cgctccttta ctgatcagct tatgtgcgcc
    12541 catcatttca ccatgatcgg aggtataaat gacccacgta ttttcacgtt gctctggcgt
    12601 taaggcattg atgacccgtc cgatttggtc atcaacaaag tcattgcagg caaaatagag
    12661 cggatggtga taaagcccgt catcaccgac tggcgatggc atcgcctgcg cccataagcg
    12721 gtgatgttcc ggtttgttcg ccaggtcatc ctctgctttc tcacccagtt cgtagtaaaa
    12781 atcagtgtat ttctctaaat actccaccgg acaggtgaac gggtgatgcg gctcatcata
    12841 cgaaatcacc atcaggaaag gttcgtcggc gcgcgcgggc tgttgcagaa aatccaccgc
    12901 ccgattgctg atgcggtgcg cccaggtgaa ggtttcgtca atatggttcg cctgtaaatc
    12961 ctcaacgctg tttaggccat tgcgccacag gctaatctct ttctccgtca gttcactaag
    13021 atagttcgcg ccatcgaacc agtaatcagc gtcccactcc ggcggacact cgccagtgcc
    13081 gaaatagtca tgtccatcga gatgccattt gccgatgtag caggtgtgat agcccgcatc
    13141 cttaaagtag cgccccatgg tggaaatgtt tttgcccggc gcgacgttgt tggtccacgg
    13201 gccggactgg ttagcgtaga taccggtgaa tagtccggcg cgtgcaggtg tacaaaccgg
    13261 tgaacaggtg taggcagaat taaagcgaat accttctgcc gccagactat caatattttg
    13321 cgtattcagc ggcttaccgc tatagcaacc gaccatattg gtggcctggg tatcggtcat
    13381 gatgaacaga aaattggggc gtttcatagg ctatccttcg catcgggagt aaaggtgacg
    13441 gctgggtcgt gacgatgccg ccagctgtca aaaatcaggt agacaatcac tacggcggcg
    13501 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc caccgaattc agccagcccg
    13561 gcatagacgc caatcatcgc gaacaggata ccaattgacg caatcttgac gtttttccac
    13621 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    13681 aaaccgatca tcagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    13741 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    13801 atcgccactt ttgccgccag cgccggaatg cgcgggaaga aaaagcccat aatgatgatg
    13861 gtaaccagcg gcacgttgta aataccgttg agctgtttca tccagctata cagcccctgc
    13921 ggcgcgttgg cgatccacgg cgcgaccatc accgaaacga cggcgataaa gaaaccaaat
    13981 ttgcgcccga cggtgaccag ctgttgcggc tcggcattct ggttaatgat gcgccggtaa
    14041 atacccatac tgaataacgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    14101 gcaccaaata acaccgcacc gaagaagccc accagtggca ccggcagaac gttattgacc
    14161 agcgtcgggt aggccatgtc ggctttcggt aaatcctgat acagatgaaa cgcaatcaac
    14221 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    14281 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    14341 caccagtaaa aggtgttcac cagaatcaaa ccggtaaatg ccgcgccaat cggcaaggga
    14401 tcggtcgggc cgccgactga gtttaatttc tcggcgtgaa cggtggtgag ttgctcaatg
    14461 ccctgcataa agctgccctt gcccatcgcg atcaggccaa ataacggcac catcaacccg
    14521 ccaataacca gcccaatacc gttaatggag tccgccactg ccattgcgcg cagtccgccg
    14581 atcaccgcat acaaaattcc cgccagacca agcaaaatca ccagtaacca gatagccgca
    14641 ccatcggaaa gttgtagcga ctcacccaca tgaaacaggc tgttgagcgc caacgcgccg
    14701 gaatagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    14761 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    14821 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac tgcagaggtg
    14881 acttcccagc ccatcaccga catgccactt ttgtaggcct gcccggaaag gccgaccagt
    14941 tgttccgtgg aaaggttggt tagcattaac gaagcggcaa taaccggcgc ttttagtgaa
    15001 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttacggac cttccaccag
    15061 gtgatgaccg ctaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    15121 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    15181 tcgggtcagg cagtcagatt cttaaaatac aaacgtcata tccctgaacg gattgttttc
    15241 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    15301 caaagttcgg atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    15361 gtgatctcca gcggaatttc tctgatatcg ctctggcata cctacgccga cgagcattat
    15421 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    15481 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg acgaacaaat aactctgcat
    15541 ggcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggtttg
    15601 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgtcggt
    15661 cagcaaagcg ttatttataa tggcgaagtt tataatcagg aactcaccga agttgctgag
    15721 ttaattactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    15781 atttatcagt ggatttgtct tatgcacgca gtcggtaaaa aagatccaca acggcggcaa
    15841 attgaaaaat taattgccac tttacatgcc aatctacaac aacgctggag cgtagctgat
    15901 atggctgcca cgatcccctg tagcgaggcc tggttgcgtc gtctgttttt acgctatacc
    15961 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    16021 aagcaacaag ggaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    16081 catttcagca aagcctttaa gcataaattt ggttatgcgc cgtcagctgt gctgaagaat
    16141 acggacaggt aataaccaga atggcgagtg tgtaaatgac tcgatgatgt tcagaaatac
    16201 caggcggagt accatttacg gcgactccac cgggaaaaga gttaaccgat actccagaag
    16261 agcaccgcca gtaattgggg tgtgataatg cgcaggaaca tcactaacgg atagacagtg
    16321 gcgtaagaga gcgccgccgc accgctggtt ggatgaagat tattagcaaa cgccagcgcc
    16381 ggaggatcgg tcatggaacc tgccagcatc ccgcacatgg tcaggtaatt cattttggct
    16441 aacatccgcg ccagaatgcc aacagtaatc agcggaacgg cggtgatcag ggcaccataa
    16501 ccaatccagc ttagcccttc gccatccacc agtgtatgga taaaatcgcc gccggatttc
    16561 aggccaacca ccgagagaaa gagcacaatg cctaactcgc gtagcgccag gttagcgctt
    16621 ggtggcataa accagtacag cttgccgata ctgccgatac gcccgaggat caacgccata
    16681 atcagcggac cgcccgccag cccgagtttc aacgccgccg ggaacccagg cacaaagacg
    16741 ggaatagaac ctaacaatac gccaagcccg ataccaataa acactggcag catctgaacc
    16801 tgttgcagtt tttgctgcgc attccccagc acattggcaa cggcatcaat tgcggacgga
    16861 cgccccacca ggttgaggat atcgccgaac tgcaggctga tatcgctact tgcaactaat
    16921 tcaacaccag cacggttcag gcgcgagata acaacgtcat agcgttcttt aaagtgcagg
    16981 tcgcgaatac gtttaccgag cacgttttca ttggtgacca ccacacgcgc gacgcgcaaa
    17041 tcagtgcctt tcgtagacag cgaggtatcg acctcctgac caatcaccag ttgcgcatta
    17101 tgtaaatccg caggctgccc caccagatgc agcaaatcgc ccagttgaat aacggtttct
    17161 ggcgaaggca cttttagggt ttcttctcgt ttcaatcgtg agcaaataac tttatcgcca
    17221 ttgagaatcg gcacatcttt aatggctaaa ttatgcaaat taggattctc aactcggatg
    17281 ttaatcgtcc tgattagcgc accgccgttg gttcgcgtgg actcgtgctg ctgggcctca
    17341 gtctcaacat tgacgcggaa aataacccgc agcatccaca tagtgaacaa aatgccgcaa
    17401 atcccaaatg gatacgccat cgcatagctc atccccattt gatcgaccat cgccatcggt
    17461 gtccccaggt cgcgcaagat ctgctgccct gcccccagcg ctggcgtatt ggtgaccgcg
    17521 ccggaaaaaa tccccagcac taccggcagt ggaatatcaa acagtttatg caggatggcg
    17581 gtaaccagac caccgatgat gacgatcaga acagcaaaca ggttgaggcg taatccggag
    17641 acgcgcaatg aggcaaagaa gcccggccct acctgaatcc cgatggtata aacgaacagg
    17701 atcaggccaa actcctgaat aacatgcagc atatcgctac ttaatgtcat ccccgcctga
    17761 gaaacaaaat ggccgacgat gatcccaccg aacagcacgc cgccaatgcc caatccaaca
    17821 ccgcgaaact tgacgttgcc gataaacaac ccgacgaccg ccaccaaagc cagaatactg
    17881 accgttaatg ctatatcact catcgtccat ttctcttgaa ataacatttt aattgctacg
    17941 gattatggct gaatcggtga agcagatttg ccttatagcg cacattatca cgggaaagaa
    18001 cgtgccgaaa tatcttaaac aggcgaaatt taacgcattg ctgaagaaaa aagacaaaaa
    18061 aaagccccac cagaatggcg gggcaaagag aatagctagt tagctattta acgcgggacg
    18121 ttcgctgata gcgatacgct gcgctgcgat gggttcaggc tcattacgaa ttaaatcaat
    18181 atgcagtaaa ccgtttacga aggttgcgcc agagacttcc atattttcag ccagcgtaaa
    18241 gctcaggcta aaaggctgat tcataagccc ttgatgcagc cattttttct cttcttttgg
    18301 ctgctctggc gtgcctttta cgctcaggcg cgtaccttcc agttgaatct ctaaatcttc
    18361 ctgacggaaa cctgccagcg caagggtaat gcggtagtgg ttatcgtcgc ttttctcaat
    18421 gttgtacggc gggaagctct ggctttcacc ggcgttttgc agtgcgttgg ccagtttgtc
    18481 aaaaccgatc cattgacgca tcagtgggga taaatcgaag ttacgcatag tcatttctcc
    18541 ttctaagaag cgagtaatta cctgcaaatc cgaagattcg catatgctcc ctgacggcga
    18601 gcatggaaat gtcaggccgc gccaggcggc cttagggaat tagttgattt cgatacggcg
    18661 cggttttttc gcttccggaa tcacgcgttc gagatcgata tacagcagac cattcaccag
    18721 gttagcacca cgaacatgaa tgttctcagc taactggaat ttacgttcaa agttgcgttc
    18781 agcgatgccc tggtacagat aggtgcgctc tttttgttcg tcggcgtgag cacctttcac
    18841 caccagcaga ttatcctggg cggtaatttc cagttcgctc tcagcaaaac cagccacagc
    18901 gatagcaatg cggtaatggt tttcgtctac cagttcaacg ttatacggag ggtagccgcc
    18961 attactctgg ctctggttgt tttctaagtg gttaaacaaa cggtcaaatc caatagcaga
    19021 acggtaaagc ggggataaat caaagttacg cataatcaat agctcctgaa atcagcgaga
    19081 atctaagacc ttccacaatg gacaggtcag gtagccagaa cacccatcag gcgcgtcctc
    19141 atcggctacg atgtaaaaat gggtctggaa atgaactttt caagccttat cagcggactt
    19201 ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc
    19261 gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact
    19321 catcaggcca acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag
    19381 cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg atgtcccata
    19441 ccggggggaa agaaggaacg tatcctggca cccgcgccag cgcaaccatg attggcgacg
    19501 atgaaaccaa ctggggcaca aaatcgctgg cgatactcga catgccattt accgcagtac
    19561 tggacacaat attgctaccg tgggatgtgt tccgcaaaga cagctcagtg cgctcgcgcg
    19621 ttgaaaaaag cgaagccgac gcgcaagaaa ccaataacgt cattcccccc gccaaaatgc
    19681 ctgcaaactg agagctagcg gtcagtttcg gtgatccaca actgaccgtc ctccatccac
    19741 gccaaccatt gaccatccgg cgagaaaacc acggcgtccg cagaaggtgg attggcgtgg
    19801 ttttcggtta aatactcaac ctcgccgctt tgcgcatggg cacaagcaat ccgattatcc
    19861 agcacaaatc ccagccattc gcctgatgga tgccagttaa atgcagactg aatatccgtt
    19921 ttattatggg ttaactggcg tggttcaccg ccctgtggcg agataagcca cagttgcaca
    19981 atgccgttat catcacgcat taaaaacgca atttgtgttc cttgaggatt acaacgcacc
    20041 cagtggcgcg gaatgttaac caggcccggg taaatacgat ggtgcgtaaa ggttaaacgt
    20101 cgctgcacga caccacgcgg tggcgcaggc agggttgttt ccgttccgct taacggcgca
    20161 tcacctgcca ctttccagcc agcttcatct tgcggcaact cgacaataaa cagctccggc
    20221 actttctcgc cctttggcga aagtgtgtcg ccaataaacg ccagtgcgtg atttcctacc
    20281 catccttctt cataagcacg attgatttca tcactgccag gctgtggcgt gggcgtggtt
    20341 ttacttacca gcacgcacca gtggctaccg ctgtattcac gcggatgctg cttttgtacg
    20401 ttgaccgggc caaacggtgc agcaacaccg acgtttcgca aatccagcgc cggatcgagt
    20461 tcatgcatta catggtcgtt gtaggtaaag ctcacccttt caccgttcgg gctaaagaca
    20521 tgtacatggc tgccgccacg tagcgctcct ggcgtatatg gcgcggtaat atccattgca
    20581 tcgagattgc tcaccttgcc gctttcaaca atcactccgc gccgatgatg aaagtcgtaa
    20641 taccatgttt catcaggatt ttccgggccg tggatgaaga catacttctc tgacttcgga
    20701 tgaacggtca ccacgccgac gtatgcgccc tgtgacgcgc gatagataac ctcgacctcg
    20761 ccggtatgga tattcacacg ctcaatggtc tcgccggtaa acgacgcccc agaaggccgc
    20821 acgtcaaaca ccagccactg gctgtcgggc gtccaggtat tggtattggt gagcaggtga
    20881 ttacggggag caaaggttat ctgtttcatg gcgcggtcct gatacaaaac ggttcccgcc
    20941 atcatacttc acaaggactt cactttcaga caatagtctt ttgccatcgt gatgaatgtg
    21001 agtgaaaaga tggaacattt cgacgtggcg attattggcc tcggcccggc agggtcggcg
    21061 ttggcgcgaa agttagcagg caaaatgcag gtgatcgcgc tggataaaaa gcaccagcat
    21121 ggtactgaag gtttcagcaa accttgtggc ggtctgctgg caccggacgc gcagcgatct
    21181 tttattcgcg atggactgac gcttcctgtc gatgtcatcg ccaacccgca gattttcagc
    21241 gtcaaaaccg tcgacgtcgc cgcatcgctc acgcgtaact accagcgaag ctatatcaat
    21301 attaatcgcc atgctttcga cttgtggatg aaatcgctga tccccgccag cgttgaggtt
    21361 taccacgaca gcctgtgtcg aaaaatctgg cgtgaggatg ataaatggca tgtcattttt
    21421 cgcgcagacg gctgggagca gcatattacc gcccgctatc tggtcggtgc cgatggtgcc
    21481 aactcgatgg tgcggcgaca tctctacccg gatcatcaga ttcgtaaata tgtcgccatc
    21541 cagcagtggt tcgcagagaa acatccggtg ccgttctact cctgcatctt tgataatgcg
    21601 ataactgact gttactcatg gagtatcagc aaagacggtt attttatctt tggcggtgcc
    21661 tatccgatga aagacggtca gacgcgtttc acggcgctga aagagaaaat gagtgccttt
    21721 cagttccagt ttggtaaggc ggtgaaaagc gaaaaatgca cggtgctgtt tccctcacgc
    21781 tggcaggatt ttgtctgcgg caaggacaac gcctttctga ttggcgaagc ggcgggattt
    21841 atcagcgcca gctcgctgga ggggattagc tatgcgctgg atagcgcaga gattctgcgt
    21901 tcggtattac tgaagctgcc agagaagctc aataccgctt actggcgcgc cactcgcaaa
    21961 ctgcgtttaa aactcttcgg caagatagta aaaagccgat gcctgaccgc accggcttta
    22021 agaaagtgga ttatgcgcag tggagtggcg catattccac agttgaaaga ttatccaacg
    22081 cgcttcacat cgcccaccag caggatgtaa gagagcgcgc caatcaacgc gacggcggag
    22141 atataaacca gtgcaggcgc gaaaccgtaa ccctgcgcaa ggtaccccac caccagcgga
    22201 acggtgatgc cgcccagacc accggcaaag ttaaacacac cgccggttaa accaatcagg
    22261 cgcatcggtg ccagagaaga gaccagcgac caggttatcg aagcaaaacc gttgccgaag
    22321 aatgccagcg ccatcaggca cataatcatc atcggatcgt tggtgtagtt cgcacccata
    22381 atgcaggtgg agatcagcaa gccgcagata atcggcgttt tacgcgcaaa gcccagtgaa
    22441 aagcccttac gtaccagcag atccgcgacc cagccagaga gcaggacgcc aataaacgcc
    22501 gcgaggaatg gcaccgtggt cataaaacct gctttcagcg ccgtgattcc tttttcctgg
    22561 gttaaatagt tcgggaacca ggttaagaaa aaccacagtg tagaagccac cgcaaattgc
    22621 ccaagataga caccgatcag tttacgatgg aacaccagtt tccagtcttt ggctgttaac
    22681 ggctgacgcg cctctttttt caccggcgca tcgccatcca ccagaccgcc gccatcacga
    22741 atgtaatcca gttcagcttt gctgatgcct ttggtcagac gcggcggctg ataaacctta
    22801 aaccaaatca gcgaccagat aatgccgata ccaccggtga caatgaacac ccagtgccag
    22861 ctcaacatct cctgaatcca gatcagcagc ggagtcagaa acgccagacc gacaaactga
    22921 ccagacgtat aaaaaccaac ggcagaagca cgttcatgtt ccgggaacca actggttacc
    22981 atccggttat tggtcgggaa cgcgggcgct tcgaaaatac cggttatcgc gcgcagacca
    23041 attaatgaca ttaagcccgt ggcaaagccc tggaataacg tcgccactga ccagccaaat
    23101 atcgcaataa aataagtcac gcgtgaacct acgcgatcta aaaaccaacc accggggatc
    23161 tgacataacg tataaagcca ggcgaaggcc gaaaatacat agcccatttg cgctttagta
    23221 ataccaaact cttcctgaat atgggcagaa gccacggcca ggttagcgcg gtcaacataa
    23281 caaatgacca ccgtaataaa gatcatcacc agcgtcagat aacgccgacg ccccggcttt
    23341 gctgcattaa cgggaatatc catagcgagc tttctccaga ttttgggcat agcgaagccg
    23401 ctcaccatgc cctgtaattt acagagggtt atatttttgt attgctgttt tagtgcccga
    23461 tgaggggctt acgtggcagg aattaccact ctgctacgct gttatcttca tgacgccaga
    23521 gcggattacg ccagtccggg gcatttttac tgaactcaat aactttagct tcgtcgattt
    23581 ccacgcctaa gcccggtttc gttaatggtt taaagaaacc gccgaccatg ctgaagtctt
    23641 ctttgttttt cacaaagtcg agtaactccg cgcctttgtt gtaatgaatg cccatacttt
    23701 gttcctgaag gacggcgtta taggaaacaa agtcgatatg caggcaagcc gccagtgcaa
    23761 tcggtccgag cggacagtgc ggcgcaaggg tcacgtcata ggcttctgcc attccggcaa
    23821 ttttgtagca ttcggtaata ccgcctgcgt gggagagatc cggttgcaga atcgaaatac
    23881 cgcctgcctc cagcacacgt ttaaaatcga agcgtgagaa catgcgctca cccgccgcca
    23941 gtggaatatg cgtttgtgcc gccagtttcg ggtagtattc ggcctgttcc gccagcaccg
    24001 gctcttcaat aaacagcggg cgatacggct ccagttcttt aatcagcact ttcgccatcg
    24061 gcgcgctgac gcgaccatgg aaatcaagac caaactcaat ctgattgcca aaagcttcac
    24121 gaatttgtgc cacggtgttg actgccgcat ctaccgcgcg ggagttatca attagcccca
    24181 gttcttcaca accgttcagt ttgaaggtat cgaagccgat ttcgcgtagc gttttaatgc
    24241 cgtcgataac atccgccgga cgatcgccgc cgacccaact gtaggcttta attttgtcgc
    24301 gaaccaggcc gcccatcagt tgccagaccg gcgcattcag cactttgcct ttgatatccc
    24361 ataacgcctg gtcaatcccg gcgatggcgc tcatcagaat cggaccgcca cgataaaatc
    24421 cggcgcgata catcacttgc cataagtcgt tgatgcgtga cgggtcctga ccaatcaaat
    24481 agtcactcag ctcgtggact gccgcttcca ccgtgcgagc gcggccttca atcacgggct
    24541 cgccccaacc gaccacgcct tcatcggttt caattttcag gaacatccag cggggaggta
    24601 aacgatacgt ggtaattttg gtgattttca ttgcactgcc tctcgatacg ccttaacaaa
    24661 tgctgctgcc tgctgcgcag tccgttctac ggactgcccg gcgcgataga gatcgctacc
    24721 taagcccgcc cctgcacaac ctgcgtctat ccactgcgcc aggttttctg gcgtcacacc
    24781 gccaacggca aagaccgcga tgtccggtgg taataccgct tttaatgctt tgatgtattg
    24841 cggaccaaaa gccgatgacg gaaatatttt cagcgcctgc gcgcccgctt cgagcgcggt
    24901 aaaggcttcg gtcgccgtcg cgcagccggg gcagacggtc atgccgtagc ccaccgcacg
    24961 gcggatcact tcactatgga tattgggcgt aacgatgagc tgacagccca ttctggcgag
    25021 cgcatcgacc tgttcaggtt tcagtaccgt acctgcgcca atcaacgcct tatcgccgta
    25081 cgcatcaacg atggcgggaa tgctctgctc ccattgtgga gaattcagcg ggatttcaac
    25141 cgcgtcgaac ccggcgtcaa tcaccgcgcc aacatgtgcc agtgcctcgt cgggcgtaat
    25201 gccgcgcaaa atggcgatca gcgggagttt agtttgccac tgcatgagcg atgctcctta
    25261 taccagcctg aaatgccgtg tcgcctgcca ccgccgtcac atcgcaaccc atcgcctgaa
    25321 aggcttgctg gtagcgcgcg gtcagcgatg ttccggcgac aagggtgatg acttgttgat
    25381 gggccacata gtcgcgcata ctggcgacct ctgcgccaat caacaaacca gagagaaatt
    25441 cgctgacctg ttcgcgggga agtgttccca gcacatgcga ggcgcgaact tcaaaaagct
    25501 gtggcaatac ggcgggcgca ttaaggccac gctcaagacc agccgcgaaa gcatcggcag
    25561 agttttcctg cggcggcaaa cctgcgccaa tcaatgagtg atttaacagt aaatgatgta
    25621 attcaccggt catcacggtg cgaaaatcgt taatttgctg gctatcggcc tgcacccatt
    25681 tgcaatgcgt accgggcatg acataaagag aggaaggagc gagagtgcgc gcgccgatca
    25741 attgtgtttc ttcgccgcgc atcacattgt ggttatcgtc atgagagacg cataatccgg
    25801 gaataatcca gatattgtcg ccagcagatg ttaattgttc gccaatagac gagaaacggg
    25861 ctggaacgga taaatacggc gcaactttcc agccgacatt gctgccgacc attcctgcca
    25921 ttaccactgg cgtattctct tcacgccagt cggtcgtcac ttctgctaac accgcagccg
    25981 gagattttcc gttcaggcgc gtgacgcctg cttctgattg cctgctgtcc aggcagtggt
    26041 cgccctgata aagccaggcg cgcagattgg tcgatcccca gtcaattgcg atgtagcgag
    26101 ctgtcatgtg atttccttta accttcgtgt cgagctggcg atcatggtaa gcgccgcctg
    26161 ctctgccgca tcgccgtcct gatgccttat cgcatcgaac agcgccttat gttcctggag
    26221 cgtttgcggc atgttggcct catcgcccat ccaggttcgt tcaaaaaccg cccgctgcag
    26281 cgaactgatc gcaatgctca gttgctgtaa caccgggtta tgcaccgact gcagcaccgc
    26341 ctcgtggtag cgaatatccg cttcgttaaa cgcttcgcgg tcctgattgt tagcaatcat
    26401 ctcgttcagc gccgattcaa tctgcgccag atcgctggag gtcgcgcgtt ctgctgccca
    26461 acgggcaatc gccggttcca ccagatttcg cacttcgctc attgcactga taagccgtgg
    26521 gtcgtagtcg ttttccagca cccattgcag tacgtcagtg tcgaggtaat tccactggtt
    26581 acgcggagcc acaaacgccc cgcgataacg tttcatttca atcagccgct tcgccatcag
    26641 cgaacgaaat acctcacgga tgatgttgcg cgaggttgca aactcctcac agagttctgc
    26701 ctcagccgga agcggcgagc ctggcacgta tttgccgtga acgatctgtc tacccagcgt
    26761 aataacgatg cgatcggttt tattgagagt catggagagt ccttgtgctc tgtatgttct
    26821 tctctacttt accccgatcg atgcataacg cggcaatttt gtagtaccag cgtgatgacg
    26881 ttcgcgtttg ccgcgcgcct aatgtagtac aaagttatat tgttgtacta caatttagat
    26941 cacaaaaaga acaatccata agaaaatgtt ctacgtcggg caaaaatctg aaaagggaga
    27001 tcaggcgcta aactgggggg aaagaagagt atgctttcaa cggcttagta ctcttgttta
    27061 aaggattaat catgaagttg aatcttaagg gatttttcaa agctgccagt ttgtgttctt
    27121 tagcatttgc gttgaccgga tgtatcacct ggggattggt ttccaatact gcaagccgtc
    27181 cacatgccga gcaatggcgg tcagacacga ttaagggttt atcgctggca gaagatagta
    27241 acggtacgaa aggctatgtt tttgttggtg aatcgttgga ctatctgttg accaccggcg
    27301 gcgatgaggt ggttaacatg ctgaacgatc cagcaatcca tggtgaaaga atcaccgttt
    27361 ctgataacgc gaaatttatt ttaagcagca gtaataagaa tttctctggt gctattacgc
    27421 tctattacga ctggaacaac gaagaagata aggcactggc aacacaatat ggttttattt
    27481 gtgatacccg gcgttgtaca tggatgctgg acggtttgaa aggctcaatc caccagaaaa
    27541 ataaaaaagc ggattactca aacgtgatgg ttttccatca accctttacg gtgggatttt
    27601 atgagtacaa agcaacagat ggcgtcccgc acggcctcgt taatgcgctg ctgccagtca
    27661 ctcttacgct ggatatagta acgtcaccac tccagttcct gattttatgc acgaccagaa
    27721 attgttaatc gctgcgggca aacgcattca tgccgtttgc ccgttcactg attaattcag
    27781 cacatacttc tcaatagcaa atgccacgcc atcttcaagg ttagatttgg tgacaaagtt
    27841 cgccacttct ttcaccgaag gaatagcgtt atccatcgcc acaccgacgc ctgcatattc
    27901 aatcattgcg atgtcgtttt cctgatcgcc aatcgccatg atttcttccg gtttaatacc
    27961 taacacgtcg gccagtgatt tcacacccgt acctttgtta acgcgtttat cgaggatttc
    28021 gaggaagtac ggcgcacttt tcagcacggt gtatttttct ttcacttcct gcggaatacg
    28081 cgcaatggcc tggtcgagga tggcgggttc atcaatcatc atcactttca ggaactgggt
    28141 attggggtcc attttctccg cttcgcagaa caccagcgga atggtggcaa caaaagattc
    28201 atgcaccgtg tagtagctga tatctcggtt ggcggtgtac agcgtggtgc ggtccagggc
    28261 gtggaaatga gaaccgactt cgcgagagag tttttccagg aaacggtagt cgtcatagct
    28321 gagagcggtt tgcgccacgg tgctaccatc agcggccttc tgtaccagcg cgccgttata
    28381 agtaatgcag tagtcgcccg gctgttccat atgcagctct ttcaggtaat tgtgaacacc
    28441 tgcataaggg cgacccgtcg ttagcacgac attcacgcca cgggcgcgag ctgcggcaat
    28501 cgcattttta acggcgggtg aaatggtgtg atcgggcagc agaagggtgc catccatatc
    28561 gatagcaatg agtttgatag ccatgagttc cccaggtaga ttggttcctg acccatgcta
    28621 acgcgattcc gctcaaaaat cagtacaaca cccgagggaa aagggggatg caacgcgcat
    28681 tgcgtgctcc ccttttgctt agcggaaaag tttccctttc agcagttcca tgcctgcgga
    28741 aagcagatcg ttattggctt gtggtgacac ttcaccttgc ggtgagagcg catcaataat
    28801 cttcggcaat tgttctgcca gtaaactgga agctgtactg gtatccacgc caagtttttg
    28861 cccgagatcg gacaccgcat ttgtgccgag cgccgattcc acttgctcgc cgctaactga
    28921 ttgattgctc tgttgattac tcagccaggt tgagagaatg gcccctaagc cgccactttg
    28981 cagtttttcc aacagcactt gaatgccgcc ctgttcctca acccagctca aaatggcctg
    29041 atatttcccc gcatcgcctt tcagaaaggc accgacaacg tcatcaaaaa ggcccatgat
    29101 aatcacctgt aaagcgttac gtgttgactc aaaaagtata gttctgtggg atgataattg
    29161 cggattgcag gaataaaaag ggcggagatg atctccgccc ttttcttata gcttcttgcc
    29221 ggatgcggcg tgaacgcctt atccggccta caaaaacatg caaattcaat atattgcagg
    29281 agtctcgtag gcctgataag cgtagcgcat caggcacgct cgcattgtta acgccattaa
    29341 atatcgatat tcgccgcttt cagagcgttc tcttcaataa acgcacggcg cggttcaacg
    29401 gcgtcgccca taagcgtagt gaacaactgg tcggcagcaa tcgcatcttt aacggtaacg
    29461 cgcagcatac gacggctttc cgggtccatg gtggtttccc acagctgttc cgggttcatc
    29521 tcgcccagac ctttataacg ctggatggag aggccgcgac gggactcttt caccagccag
    29581 tccagcgcct gctcgaagct ggctaccggc tgacgacgtt cgccacgttc gataaatgca
    29641 tcttcttcca gcaagccacg cagtttctca cccagcgtgc agatacgacg atattcgccg
    29701 ccagtgataa attcgtgatc cagcggatag tcagtatcca caccgtgggt acgcacgcga
    29761 acaatcggct cgaacaagtt ttgctcagca ttggtgtgga catcaaactt ccactggctg
    29821 ccgtgctgtt ctttgtcgtt cagttcgctg accagcgcgt tcacccagcg ggtaacggtc
    29881 tgctcatcag agagatcggc ttccgtcagc gtcggctgat agataagctc tttcagcatt
    29941 gctttcggat aacgacgctc catgcggttg atcattttct gcgtcgcgtt gtactcagac
    30001 accagtttct ctaacgcttc gccagccagc gccggtgcac tggcgttggt gtgcagcgtt
    30061 gcgccatcca gcgcgataga gatctggtac tgatccatcg cttcgtcgtc tttaatgtac
    30121 tgttcctgct tacctttctt cactttgtac agcggcggct gagcgatgta gacgtgacca
    30181 cgttcaacga tttccggcat ctgacgatag aagaaggtca acagcagcgt acgaatgtgc
    30241 gagccgtcga cgtccgcatc ggtcatgatg atgatgctgt gataacgcag tttgtccggg
    30301 ttgtactcgt cacgaccgat accacaacca agcgcggtga taagcgtcgc cacttcctga
    30361 gaagagagca tcttatcgaa gcgcgctttc tcgacgttga ggattttacc cttcagcggc
    30421 agaatcgcct ggttcttgcg gttacgcccc tgcttcgcag agccgcccgc ggagtcccct
    30481 tccacaaggt acagttcgga aagcgccgga tcgcgttcct ggcagtctgc cagtttgccc
    30541 ggcaggccag ctaaatccag cgcaccttta cggcgggtca tttcacgcgc gcgacgcgca
    30601 gcttcacggg cacgggcagc atcgataatt ttgccgacca cgattttcgc gtcggttggg
    30661 ttttccagca ggtattccgc cagcagttcg ttcatctgct gttcaaccgc cgatttcacc
    30721 tcagaagaaa ccagtttgtc tttggtctgt gaggagaatt tcgggtccgg cactttcacg
    30781 gaaacgaccg caatcaggcc ttcacgcgca tcgtcaccgg tggcgctgac tttggctttt
    30841 ttgctgtagc cttctttgtc catgtaggcg ttcagggtgc gggtcatcgc cgcacggaag
    30901 cctgccaggt gagtaccgcc gtcacgctgc ggaatgttgt tggtaaagca gtagatgttt
    30961 tcctggaagc catcgttcca ctgcaacgcc acttcgacgc caataccgtc tttttcggtg
    31021 gagaagtaga agatattcgg gtggatcggc gttttgttct tgttcagata ttcaacgaac
    31081 gccttgatgc cgccttcata gtggaagtgg tcttctttgc cgtcgcgctt gtcgcgcaga
    31141 cgaatggaaa cgccagagtt gaggaacgac aactcacgca gacgtttcgc cagaatgtca
    31201 tattcgaact cggtcacatt ggtgaaggtt tcaaggcttg gccagaaacg caccatagtg
    31261 ccggtttttt cagtctcgcc ggtaaccgcc agcggggcct gcggtacacc gtgttcgtag
    31321 atctgacggt gaattttacc ctcgcgctgg ataaccagct ccagtttttg cgacagggcg
    31381 tttactaccg aaacaccaac gccgtgcaga ccgccggaca ctttatagga gttatcgtca
    31441 aatttaccgc ctgcgtgcag aacggtcatg atcacttccg ccgccgatac gccctcttcc
    31501 gggtgaatac cggtcggaat gccgcgcccg tcatcctgta cagagacaga gttatcggcg
    31561 tgaatggtga cgataatttc tttacagtga cccgcgagcg cttcgtcgat agcgttatct
    31621 accacctcga ataccatgtg gtgcagaccg gtgccgtcat ccgtgtcgcc gatatacata
    31681 cccgggcgct tacgcaccgc atccagccct ttcaggactt tgatactgga ggagtcataa
    31741 gaattcgaca tcaacgtttc tcgctcattt atacttgggt taatccgtta ttttaccctt
    31801 ttccacggta aacatcttcg aattttcgtc cgacatgtct ataacgtgtt cagcactgat
    31861 cgcgctgaca aagacctgtg attgcgtcgc ttttaagcgg ctggcaagca gcccgcgacg
    31921 ctcatcatca agctcagagg caaaatcatc tatcaggtag agacaccgcc gcccgctttc
    31981 acgggtgagg aactctcctt gcgccagacg taaggcgcac atcaacagct taagctgccc
    32041 acgcgataag gtatcttcca ccggcgcacc gtcggcgcga atgcgtaagt ccgctttatg
    32101 cgggccgtgc gcggtgtagg ttagctggcg atcgcgttca aaattacgtt ccagcacctc
    32161 agcgtactct gtctctttct cccagccgcg ctggaaagag aaagtcagag aaaactcagg
    32221 gagaaattgc ttacaggtat cagccatatc ggctgcgata ccggcgctat actccgcgcg
    32281 ccaggtgctg atttgctccg ccagcgggat cagttcttta tcccacgggc gtagctgttc
    32341 gtaacgagtc acctggcgca gcgccgcatt gcgctgcttg agcaatcgct tgagattgct
    32401 ccaggcggtg aaaaatccgg gttcgttgtg aaagcatccc cagtcgagga atgctcttct
    32461 gtatttgggg ccgccgttga gtaaagtaaa cccttctggc gttatcagct gcattggcat
    32521 caggtgcgcc agctccgcga ctttatgccc gtctgtaccg tcaatacgga ctttgctatc
    32581 gccttgtttg tctttggtta aaccaatcgc cgtctcgcgc tcttcgccct gtaatcgccc
    32641 gtggagaaca aacgcctcct gctcgtggcg aatgacgcga ccaatctgca aactgcgaaa
    32701 cgcccgaccg tggccgagcg tatagatggc ttccagcacg ctggttttgc cactgccgtt
    32761 ggcacctacc agaaagttaa agccgggaga taaggcgaga tccgcggttt caatgttgcg
    32821 gaaatcgcgg atcaacaagc gggtgaggga cattacagtc tcattggcat gacaacataa
    32881 gccgcgctct ggctggccgc atcttcaatc tgcacgctgg aaaccgaatc ggtcagcatc
    32941 atgcggacgt tttcgcattt cagcgcgttg agaacatcca gcacatagct gacgttgaag
    33001 ccgatttcca tctccgcacc gctataggta acgtcgagga tctcttccgc ttcttcctgt
    33061 tctgggttgt tggcggtgat tttcagctgg ttttcgctga catacagacg cacgccgcgg
    33121 aatttctcgt tagagagaat tgccgcacga gcaaacgcct gcttgagcag atcacagcca
    33181 gcttccagat gtttgtccgg gttcttcggc agaacgcgac ggtaatccgg gaagcgacca
    33241 tccaccagtt tcgaggtgaa gataaagtcg ccaacgtggg cgcgaatgtt gttgctgcca
    33301 atctgcacgc gcagcggatt gtcgccgccg tcgagcatac gcatcagttc aatcacgcct
    33361 ttacgcggca cgatcaccga atggcttggc aaagattgac caattggcat tgaacagact
    33421 gccagacggt ggccgtcggt cgccacggtg cgcagttctt caccttcggt ttcaaacagc
    33481 ataccgttta agtaatagcg aacgtcctga tgcgccatag aaaactgggt cgcttcaatc
    33541 agacgcttca tcgtcgcctg cggcagggta aattcgactt cgctctgcca gtcatcgagg
    33601 ttcgggaaat ctgccgctgg cagggtagac agcgaaaaac ggctacgccc ggagcgcacc
    33661 agcatccgct caccttccag ctgcacggca atttccgcgc cttcaggcag accacggcag
    33721 atatcaaaga atttgcgcgc cggaacggtc gtcgcccctg gttcgtgcgg ctgaaccagc
    33781 gcaacgcgtg ccaccatttc catctcgaga tcagtaccgg tcagcgacaa cgtaccgtca
    33841 gcaacctgta acagcagatt accgagaatc ggtagcgtag gacgaccacc taacggaccg
    33901 ctcacctgtt gtagcggttt taataaatgc tcacgttcta cggtaaattt cataggttta
    33961 cgatgacaat gttctgatta aattagaaaa atcttctttg atatcgtggc tctcttcacg
    34021 caactgctcg atcttccggc aggcatgaag caccgtcgtg tggtcacgac caccaaaggc
    34081 atcgccaatc tccggcagac tgtggttagt cagctctttc gccagcgcca tcgccatctg
    34141 gcgcggacgg gccaccgagc gggatcgacg cttggaaagg agatccgcga ctttgatctt
    34201 gtagtactcc gccaccgtct tctgaatatt gtcgatggtg accagttttt cctgcaatgc
    34261 cagcaagtcg cgcagcgcct cacgcacgaa gtcgatggtg atcgcccgtc cggtaaagtt
    34321 ggcattggca atgacgcggt tcagcgcgcc ttccaactca cgtacgttag atcgtagacg
    34381 cttggcgata aagaacgcca cttcgcccgg caaacgaatg tcgttttcgt cggccttttt
    34441 catcaggatc gccacgcggg tttccagctc tggcggttcg atcgccacag tcagtcccca
    34501 gccgaagcgg gatttcaaac gatcctcaac gccgttgatc tctttcggat agcgatcgga
    34561 ggtgagaatg atctgttgat taccttccag cagggcgttg aaggtgtgga aaaactcttc
    34621 ctgagatcgt tctttattag caaaaaactg aatatcgtcg atcagcagtg catctacgga
    34681 acggtagtag cgtttaaact cttcgatcgc gttgttttgc agggctttaa ccatgtcctg
    34741 aacaaagcgc tcggagtgca tataaaccac tttggcattc ggcttgcgcg ccataatgcc
    34801 gttacccacc gcatgcagca ggtgagtttt acccaaaccc gtgccgccat aaaggaacaa
    34861 cgggttataa gcaccgccag ggttatctgc cacctggcga gccgccgcgc gcgccagttg
    34921 gttagattta ccttcaacga agttatcaaa cgtgtgtttg acgtttacgt tagaacgata
    34981 ggtcggttct gccggagccg ggacgttatc ccaacccgag cgcgtagaag gcgcagcacg
    35041 ttgcggctgc gtttgcgcca cctgtgcagg ggccgcgacg ttgctcgtca ctgccgcttg
    35101 tggcgtttgc gtcaccggtt tggtgccgac ttcaaaacgc agctgtgggg catccgctcc
    35161 gcagaaactg gttagcagtc cattgatatt attaaggtac ttgtcccgta cccaatcgag
    35221 gacaaaacgg tttggcgcgt acagggccag cgtgttatcg ctcagttccg cctgcaatgg
    35281 gcgtatccac atactgaatt ctgtggctgg taactcatcc tgcaatcggg caagacactg
    35341 ctgccaaagc gaaagtgaca cggcggactc cactcgaaca aaagtcgata atgactaagg
    35401 ctgaaacatt catgattgtt gacgtacgtc gaaaagaccc tgcatgaggg tgacgcacga
    35461 accgctgtct gcggttatat gcccgatcaa gatcctgcaa aacgatcggg accgcggatc
    35521 atagcctaaa ctgcgcaaga gatcttctgt ttctcacaga ttttcccgat ttatccacag
    35581 gatgatccag aactcgctaa gtgtaaacga tcctgccgcg ggccgggcac gatttacgcc
    35641 gcatattgga aaatttaatg accatagaca aaaattggct taatcgatct aataaagatc
    35701 caggacgatc cttgcgcttt acccgtcagc ccgtataatc ctccacccgg cgcgccatgc
    35761 tggttttcac tggtgtgagg ccgtacgttt tccccgcgaa aaggtgcgga aaagcgcggt
    35821 aaataaggaa agagaattga ctccggagtg tacaattatt acaatccggc ctctttaatc
    35881 acccatggct tcggtgtcca tcgtttcatt tttcggcgga tatccaataa agccattgaa
    35941 tttattcaag tttaggtaga aatcgccatg aaacgcactt ttcaaccgtc tgtactgaag
    36001 cgcaaccgtt ctcacggctt ccgtgctcgt atggctacta aaaatggtcg tcaggttctg
    36061 gcacgtcgtc gtgctaaagg ccgcgctcgt ctgaccgttt ctaagtaata aagctaaccc
    36121 ctgagtggtt aagctcgcat ttcccaggga gttacgcttg ttaactccca gtcaattcac
    36181 attcgtcttc cagcagccac aacgggctgg cacgccgcaa attaccattc tcggccgcct
    36241 gaattcgctg gggcatcccc gtatcggtct tacagtcgcc aagaaaaacg ttcgacgcgc
    36301 ccatgaacgc aatcggatta aacgtctgac gcgtgaaagc ttccgtctgc gccaacatga
    36361 actcccggct atggatttcg tggtggtggc gaaaaaaggg gttgccgacc tcgataaccg
    36421 tgctctctcg gaagcgttgg aaaaattatg gcgccgccac tgtcgcctgg ctcgcgggtc
    36481 ctgatagccc tcattcgggt ctatcaacgc ctgattagtc cgctactcgg gccgcattgt
    36541 cgtttcactc caacctgttc aagctacgga attgaggcat tgcgcaggtt tggagtgata
    36601 aaaggcagtt ggttgacggt gaaacgcgta ttaaaatgcc accctttaca ccctggtggt
    36661 gacgatcccg tcccgcccgg accatttgat accagagaac actaacgatg gattcgcaac
    36721 gcaatctttt agtcatcgct ttgctgttcg tgtctttcat gatctggcaa gcctgggagc
    36781 aggataaaaa cccgcaacct caggcccaac agaccacgca gacaacgacc accgcagcgg
    36841 gtagcgccgc cgaccagggc gtaccggcca gtggccaggg gaaactgatc tcggttaaga
    36901 ccgacgtgct tgatctgacc atcaacaccc gtggtggtga tgttgagcaa gctctgctgc
    36961 ctgcttaccc gaaagagctg aactctaccc agccgttcca gctgctggaa acttcaccgc
    37021 agtttattta tcaggcacag agcggtctga ccggtcgtga tggcccggat aacccagcta
    37081 acggcccgcg tccgctgtat aacgttgaaa aagacgctta tgtgctggct gaaggtcaaa
    37141 acgaactgca ggtgccgatg acgtataccg acgcggcagg caacacgttt accaaaacgt
    37201 ttgtcctgaa acgtggtgat tacgctgtca acgtcaacta caacgtgcag aacgctggcg
    37261 agaaaccgct ggaaatctcc acctttggtc agttgaagca atccatcact ctgccaccgc
    37321 atctcgatac cggaagcagc aacttcgcac tgcacacctt ccgtggtgcg gcgtactcca
    37381 cgcctgacga gaagtacgag aaatacaagt tcgataccat tgccgataac gaaaacctga
    37441 acatctcttc gaaaggtggt tgggtggcaa tgctgcaaca gtatttcgcg acggcgtgga
    37501 tcccgcataa cgacggtacc aacaacttct ataccgctaa tctgggtaac ggcatcgccg
    37561 ctatcggcta taaatctcag ccggtactgg ttcagcctgg tcagactggc gcgatgaaca
    37621 gcaccctgtg ggttggcccg gaaattcagg acaaaatggc agctgttgct ccgcacctgg
    37681 atctgaccgt tgattacggt tggttgtggt tcatctctca gccactgttc aaactgctga
    37741 aatggatcca tagctttgtg ggtaactggg gcttctccat tatcatcatc acctttatcg
    37801 ttcgtggcat catgtacccg ctgaccaaag cgcagtacac ctccatggcg aagatgcgta
    37861 tgctgcagcc gaagattcag gcaatgcgtg agcgtctggg cgatgacaaa cagcgcataa
    37921 gccaggaaat gatggcgctg tacaaagctg agaaggttaa cccgctgggc ggctgcttcc
    37981 cgctgctgat ccagatgcca atcttcctgg cgttgtacta catgctgatg ggttccgttg
    38041 aactacgtca ggcaccgttt gcactgtgga tccacgacct gtctgcacag gacccgtact
    38101 acatcctgcc gatcctgatg ggcgtaacga tgttcttcat tcagaagatg tcgccgacca
    38161 ctgtgaccga cccgatgcag cagaagatca tgacctttat gccggtcatc ttcactgtgt
    38221 tcttcctgtg gttcccgtca ggtctggtgc tgtactatat cgtcagcaac ctggtaacca
    38281 ttattcagca gcagctgatt taccgtggtc tggaaaaacg tggcctgcat agccgcgaga
    38341 agaaaaaatc ctgattcggt gagttttcgc taaaataagg gcggtcagtt gaccgccttt
    38401 tttcttttcg tagggcggat aagcaccgca catccgccac acaaagcaac aggaacatca
    38461 tgagcgataa tgacactatc gtagcccagg ccacgcctcc gggacgtggc ggcgttggca
    38521 tcctgcgcat ctccggcctc aaagcccgtg aagttgccga aaccgtgctg ggtaaactgc
    38581 ctaagccacg ctacgccgat tatcttccgt ttaaagactc cgatggcagc gtgctcgatc
    38641 aggggattgc gctatggttc cctggcccga actcgttcac cggcgaagat gtgctggaac
    38701 tgcaaggtca tggcggtccg gtgatcctcg acctgctgtt aaaacgcatt ctgaccattc
    38761 ccggcctgcg gattgcccgc cctggtgagt tttccgaacg cgcatttctg aacgataaac
    38821 tcgacttagc ccaggccgag gcgattgcgg atcttatcga cgccagctcg gaacaggcgg
    38881 cccgttcggc gctaaactcg ctgcaaggcg cgttctccgc acgggtcaac catctggtgg
    38941 aagccctcac ccacttgcgc atttacgtcg aagcggcaat tgatttcccg gatgaagaga
    39001 tcgatttcct ctccgacggc aaaattgaag cccagctcaa taacgtaatt gccgatctcg
    39061 acgcggtacg cgccgaagct cgtcagggta gtttgttgcg cgaagggatg aaagtggtga
    39121 ttgccggacg tcctaacgcc ggtaaatcga gcctgctgaa cgcgctggcg ggccgtgaag
    39181 cagcaatcgt aaccgatatc gccggaacca cgcgtgacgt gctgcgtgag catatccaca
    39241 ttgacggaat gccgttgcat atcatcgata ccgccgggct acgtgaagcc agtgacgaag
    39301 ttgaacgtat tggtatcgag cgcgcatggc aggaaattga acaggcagac cgcgtgctgt
    39361 ttatggtcga tggcaccacg acagacgccg ttgatccggc agagatctgg ccggaattta
    39421 tcgcccgtct gccagcgaaa ctgccgatca ccgtggtgcg caataaagcc gatatcaccg
    39481 gcgaaacgtt ggggatgagc gaagtgaacg gtcacgcgtt aattcgtctt tcggcgcgga
    39541 ctggcgaagg tgtggatgta ctgcgtaacc atctcaaaca gagcatgggc tttgacacca
    39601 atatggaagg cggcttcctg gcgcgtcgtc gtcacctaca ggcgctggaa caggcggcag
    39661 agcatttgca gcaaggtaaa gcgcaactgt taggtgcctg ggcgggtgaa ctgctggcgg
    39721 aagagttgcg cctggcgcag cagaacttaa gcgagatcac cggggaattt acttcagacg
    39781 acctactggg gcggattttc tccagcttct gtatcggtaa gtaaccgcgc ttgcgaagcc
    39841 gcattctgac tgtcagatgc ggcttcgctt cattgttagc actcctgtta ttcctcaacc
    39901 cttttttaaa cattaaaatt cttacgtaat ttataatctt taaaaaagat atttaatatt
    39961 gcgtcccgaa cgattgtgat tcgattcaca tttaaacaat ttcagaatag acaaattcct
    40021 tgagtattat actgtagcct cgtgtctttc gaggataagt gcattatgaa tatcttacat
    40081 atatgtgtga cctcaaaatg gtttaatatt gacaacaaga ttgtcgatca ccgcccttga
    40141 tttgcccttc tgtagccatc accagagcca aaccgattag attcaatgtg atcgattagt
    40201 ttgctatatc ttaattttgc cttttacaaa gaccatctct cgtttattta cttgttttag
    40261 taaatgatgg tacttgcata tatatctggc gaattaatga atattgcaaa tgtaatattc
    40321 acagggatca ctgtaattaa aataaatgaa ggattatgta atggaaaact ttaaacatct
    40381 ccctgaaccg ttccgcattc gtgttattga gccagtaaaa cgtaccactc gcgcttatcg
    40441 tgaagaggca attattaaat ccggtatgaa cccgttcttg ctggatagcg aagatgtgtt
    40501 tatcgattta ctgaccgaca gcggcaccgg ggcggtgacg cagagtatgc aggccgcgat
    40561 gatgcgcggc gacgaagctt acagcggcag ccgcagctac tatgcgttag ccgagtcagt
    40621 gaaaaatatc tttggttatc aatacactat tccgactcac cagggccgtg gtgcagaaca
    40681 aatctatatt ccggtactga ttaaaaaacg cgagcaggaa aaaggcctgg atcgcagcaa
    40741 aatggtggcg ttctctaact atttctttga taccacgcag ggccatagcc agattaacgg
    40801 ctgtaccgtg cgtaacgtct atatcaaaga agccttcgat actggcgtgc gttacgactt
    40861 taaaggcaac tttgaccttg agggattaga acgcggtatt gaagaagttg gcccgaataa
    40921 cgtgccgtat atcgttgcaa ccatcaccag taactccgca ggtggtcagc cggtttcact
    40981 ggcaaactta aaagcgatgt acagcatcgc gaagaaatac gatattccgg tggtaatgga
    41041 ctccgcacgc tttgccgaaa acgcctattt catcaagcag cgtgaagcag aatacaaaga
    41101 ctggaccatc gagcagatca cccgcgaaac ctacaaatat gccgatatgc tggcgatgtc
    41161 cgccaagaaa gatgcgatgg tgccgatggg cggcctgctg tgcatgaaag acgacagctt
    41221 ctttgatgtg tataccgaat gcagaaccct ttgcgtggtg caggaaggct tcccgacata
    41281 tggcggcctg gaaggcggcg ctatggagcg tctggcggta ggtctgtatg acggcatgaa
    41341 tctggactgg ctggcttatc gtatcgcaca ggtgcagtat ctggtcgatg gtctggaaga
    41401 gattggcgtt gtctgtcagc aggcgggcgg tcacgcggca ttcgttgatg caggtaaact
    41461 gttgccgcat atcccggcag atcagttccc ggcacaggcg ctggcctgcg agctgtataa
    41521 agtcgccggt atccgtgcgg tagaaattgg ctctttcctg ttaggccgcg atccgaaaac
    41581 cggtaaacaa ctgccatgcc cggctgaact gctgcgttta accattccgc gcgcaacata
    41641 tactcaaaca catatggact tcattattga agcctttaaa catgtgaaag agaacgcggc
    41701 gaatattaaa gggttaacct ttacctacga accgaaagta ttgcgtcact tcaccgcaaa
    41761 actgaaagaa gtttaattaa aacactcaga gtggctataa ggatgttagc cactctatta
    41821 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaat
    41881 cagaaaaaaa acactctgca ttttggggtg ttatggttat agccggtaca gtaattggcg
    41941 gtgggatgtt tgctttacct gttgaccttg ccggagcctg gtttttttgg ggcgctttta
    42001 ttcttattat cgcctggttt tcaatgttac attcagggct attattatta gaagcaaact
    42061 taaattatcc cgtcggatcc agttttaaca ccatcaccaa agatttaatc ggtaacacct
    42121 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
    42181 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
    42241 atccacgtat tgtcgggatc tgcactgcca ttttcgttgc cagcgtattg tggataagtt
    42301 cgttagccgc cagtcgtatt acctcattgt tccttgggct gaagattatc tcctttgtaa
    42361 ttgtttttgg ttctttcttc ttccaggtcg attactccat tctgcgcgat tccaccagca
    42421 ccactgcggg gacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
    42481 catttggttt ccacggcaat attcccagcc tgattatttg ctatggaaaa cgcaaagata
    42541 agctcatcaa aagcgtggtc ttcggttcgc tgctggcgct ggtgatttat ctcttctggc
    42601 tctattgcac gatggggaat atcccgcgag aaagctttaa ggcgataatc tcctcaggcg
    42661 gcaacgttga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
    42721 tttgcctgct ggtgttctct aacttagctg ttgccagctc gttttttggt gtcacgctgg
    42781 gattgttcga ttatctggcg gatctgttta agattgataa ctcccacgcc gggcgtttca
    42841 aaaccgtgct gttaaccttc ctgccacccg cgttgttgta tctgatcttc ccgaacggct
    42901 ttatttacgg gatcggcggt gccggactgt gcgccaccat ctgggcggta ataattcctg
    42961 ctgtactggc aatcaaagcc cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
    43021 gcaatcttat tccggcgatt gtcattctct ttggcataac cgtaatttta tgctggttcg
    43081 gtaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaggaagcc agccattcgc
    43141 tggcttcttg cctgtcagga aatcacttat gtccaaatgg caactcgcct gattctcctt
    43201 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
    43261 gatttgtagt tttgccctgg ttttacttta tcccgcaggg attgatatgt acctcgttgg
    43321 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
    43381 cgtttatctg gcggggatgg cggctgcgat gttatttgcc ggtaaagtgg ccgatcgttc
    43441 agggagaaag cctgtcgcca tacccggcgc ggcgctattt attatcgcct cggtgttctg
    43501 ttcactggct gaaaccagcg cgttatttct tgcagggcga tttctacagg gactgggcgc
    43561 gggctgttgt tacgtagtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
    43621 ggctaaagtg ctgtcgttac tcaacggcat tacctgcatc attccggtgt tagcgccggt
    43681 gctcggacac ctgattatgc ttaaattccc ttggcagagt ctgttctgga cgatggcaac
    43741 gatgggcatc gcggtactga tgctgtcttt gtttatttta aaagaaacgc gccctgcggc
    43801 ccccgcagct tcggacaaac cacgagaaaa tagcgagtca ctgcttaatc ggtttttcct
    43861 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcaccttcg tcaacacgtc
    43921 gccggtattg ctgatggaaa tcatgggttt tgagcgcgga gaatacgcca ccattatggc
    43981 gttgaccgct ggcgtcagca tgaccgtttc attctccacg ccgtttgcgc tgggaatttt
    44041 taagccacgt acgttgatga tcacctcaca ggtgttattc ctggcggcgg ggatcaccct
    44101 tgctgtttca ccttcccatg cggtttcgct gtttggtatc acgctgattt gcgccggttt
    44161 ctcggtaggt tttggcgtag cgatgagtca ggcgttaggg ccgttttcat tacgcgcggg
    44221 cgtagccagc tcgaccttag gtattgcgca agtttgcggt tcgtcactgt ggatttggct
    44281 ggcggcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
    44341 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
    44401 agaaatccat caccacgctt gatctcaatt tgctgctctg tctgcaactg ctgatgcagg
    44461 agcgcagcgt gaccaaagcg gcgaagcgga tgagcgtgac gccttcggcg gtgagtaagt
    44521 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
    44581 tgtcgcccac accgctaatg gtcagcatgg agcaaaatct ggcggagtgg atgcaaatga
    44641 gcaacctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgaactgg
    44701 cagcagaatc accgctaatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
    44761 gttacccgca ggcgaccatc aaattacgta actgggatta cgattcctta gatgccatta
    44821 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag tcatccccgt tcgcgggagc
    44881 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtgccctgcg
    44941 tctggttacg caaagatcat ccggcactgc atgaagcatg gaatctggac acctttttac
    45001 gttatccgca tatcagcatt tgctgggaac agagcgatac ctgggcgctg gacaatgtgt
    45061 tacaggagct ggggcgcgaa cgcacgattg ctatgagcct gccggaattc gagcagtcac
    45121 tgtttatggc agcacagccc gacaatctgc tactggcgac cgcgccgcgc tactgccagt
    45181 actacaatca actccaccaa ctgccgttgg ttgctcttcc tctcccgttt gatgaaagcc
    45241 agcaaaaaaa gctggaagtc ccttttaccc ttctgtggca taaacggaac agccgtaatc
    45301 cgaagatcgt ctggttacgg gaaaccatta aaaatcttta cgcgtcgatg gcataaccga
    45361 atcgtatgaa acgcgaccca atttcacaat gaaatgtaaa aaagttgtaa taaacttatc
    45421 tgaatcgaac ttttagccgc tttagtctgt ccatcattcc agtaaatgat tactcttgta
    45481 ttcataatgg accattaagc atggagcgaa aaatggcgac tcactttgcc cgagggattt
    45541 taacggaagg acatctgatt tctgttcgtc tcccctccca gtgtcatcaa gaagcccgaa
    45601 acattccccc tcatcgtcaa agccgttttc tggcgtccag aggtttactc gcagaactga
    45661 tgttcatgct gtatggcatt ggcgaattgc cggaaatcgt caccctgccg aaaggaaaac
    45721 cggttttcag cgataaaaat ttgccttcgt tttctatttc ctatgccggg aatatggtcg
    45781 gcgtggcgtt aacaaccgaa ggtgaatgtg gcctcgatat ggaactacag cgtgcgacac
    45841 gcgggtttca tagcccacac gcgcccgata accacacctt ttccagcaat gaatcgctat
    45901 ggatcagtaa acaaaacgat cctaacgaag cgcgggcgca gctcatcacg ctgcgccgaa
    45961 gcgtgctaaa actaaccggt gatgttttga atgacgatcc gcgcgatctg caactactgc
    46021 ccattgccgg acgcctgaaa tgtgctcatg taaatcatgt agaagcgtta tgcgacgcgg
    46081 aggacgtgct ggtgtggtcc gtggcggtca cgcccacgat tgaaaagctc agtgtctggg
    46141 agttagatgg caaacacggc tggaaaagcc tgcccgatat tcacagccgc gccaacaatc
    46201 ctaccagccg gatgatgcgt tttgcccagc tctctaccgt gaaggctttt tcgccaaatt
    46261 gatagacaac cacaggagtc atcatgtctg aaaaattgca ggtggttacg ttactgggga
    46321 gcctgcgcaa aggctcattt aatggcatgg ttgcacgtac cctgccgaaa attgctccgg
    46381 cgagcatgga aatcaatgcg ttaccttcca ttgccgacat tcccctgtat gacgctgacg
    46441 tacagcagga agacggtttt ccagcaacag ttgaagctct ggcggaacag atccgtcagg
    46501 ctgacggtgt ggtaatcgtc acgccggaat ataactactc ggtaccgggt gggctgaaaa
    46561 atgccatcga ctggctttcc cgcctgccgg atcaaccgct ggcaggtaaa ccggtattga
    46621 ttcagaccag ctcaatgggc gtgattggcg gcgcgcgctg tcagtatcac ctgcgccaga
    46681 ttctggtttt cctcgatgca atggtgatga acaagccaga atttatgggc ggcgtgattc
    46741 agaacaaagt tgatccgcaa accggggaag tgattgatca gagtacgctg gaccacctga
    46801 ccgggcaatt gaccgcattt ggtgagttta ttcagcgagt taagatctaa ataaaaaacc
    46861 cgccagcaat catgcatggc gggtttttaa cgcgctatcg attttagtga gcgtcgataa
    46921 agacaatctt caggataaac agcagcgcaa cgatgattac gcacgggcta aggtcacgca
    46981 gacgtccggt accaatcttc atcacgcagt aggagataaa gcccagcgcg ataccttcag
    47041 taatcgagaa gctgaacggc atcatcacag cggtaataaa cgccggaaca gattcagtaa
    47101 gatcctgcca gttcacgcga gcaagacttg aggtcatcaa cacgccaacg taaatcagcg
    47161 caccagctgc agcgtagcct ggcaccatcc ccgccagcgg cgacaggaag ataaccagca
    47221 ggaacagcag accaacaacc actgccgtca gaccggtacg accgccaacg gaaacgccgg
    47281 aagaagactc gatatatgcc gtaacagaag aagtcccgat aaacgaaccg gtcacggaag
    47341 agatgctgtc gacatacagt gcctgcttca tgcgcgggaa tttacctttt tcatccgcca
    47401 gtcctgcttt atcggtcacg ccaatcagtg taccggagga gtcaaacagg ttgaccagca
    47461 tgaaagagaa aatcacccct gccagcccga ggttaaacga cccggctaaa tctacatgac
    47521 ccacgacagt cattacgctc ggcggcgcag aaacgatgcc attgtagtgg acatcaccca
    47581 gcatccagcc cagcagcgtc gtcaccacga tggaaaccag caccgccgcg tgaatgttgc
    47641 gtgaggccag aatagcaatg atgaagaagc cgaggatccc cagcagcacg ctgtgagaag
    47701 tcagattacc gatgctcacc agcgtttccg ggttagcgac aatcacacct gcgtttttca
    47761 gccccatcat gccaataaac agaccgatac cactggtgat gcccacacgt agacttaccg
    47821 ggatgttggc tatcatccag tagcgaacgc ggaaaatcgt cagtaacagc agaccaatcg
    47881 cgccccagaa gattgcgccc atcccgactt gccacggcaa gcccatcgcc tgtacaacca
    47941 caaaagcgaa gaacgcattc aggcccattg ccggtgccag tgcaactggc aggttagcaa
    48001 acagccccat cataatactg ccgaatgcag caatcagaca ggtcgtgacg aagacggcgc
    48061 tggtatccat gccagcaacg ccaagaattt gcgggttaac aaaaacgatg taaaccatcg
    48121 tcaggaaggt ggtaaaaccg gcgatcactt cggtccgtgc cgtcgtgcca tgttcgcgca
    48181 gtttaaacac gcgttccagc atcccctggc cagaagcctg ggttgtgtgt tgttgactca
    48241 ttatctattt ccgaacaagg agggaaaatc cgtcgctatc gtataccaaa atgagacaat
    48301 atggacggct gaatataact ttttttcatt gtatttgctt atacggcaac gattgcgtca
    48361 tgcgaattgg catgacgaaa acgttaaact aacgataaag gaaagacatg tcccagatag
    48421 aagcggtatt tttcgactgc gacggtacgc tggtcgatag cgaggtcatt tgctctcgtg
    48481 cctatgtcac gatgttcagg gaatttggca ttcacgttga cctggaagag atttttaccc
    48541 gttttaaagg cgtaaaactc tacgaaatca tcgacatcat cagtaaagaa cagggcgtga
    48601 cgatggtcca ggccgatgcc gagcacgttt accgcgcaga agtagcacgt ctgtttgata
    48661 ccgaacttga ggacatcgca ggagcgaacg aattgctggc aagcattaac gtgccgatgt
    48721 gcgtcgtctc taacggtccg gtcagcaaaa tgcagcattc gcttggtaag ctcaacatgc
    48781 tgcactactt cccggaaaaa ctgttcagcg gctatgatat ccagcgttgg aagcccgatc
    48841 cggcattaat gttccatgcg gcaaaagcga tgaatgtgaa cgttgaaaac tgcattctgg
    48901 tcgatgactc cagcgccggt gcgcaatcag ggatagatgc cggaatggaa gtgttttact
    48961 tctgcgctga cccgcacaat aaaccgatcg ttcacccgaa agtcaccacc tttacccatc
    49021 tttcgcaatt accggaactg tggaaggcgc gtggttggga tattacggca tagttattca
    49081 cactcccttc acttactcca cttaaattgg cacgcaaagg taagtaaagg gagtttgata
    49141 tgtctgtttc acgtcgggta attcatcacg gactgtattt tgcggtttta gggccgttaa
    49201 ttggtgttct gtttcttgtc ctctacatat tcttcgcaaa agaaccgctg attcttttgg
    49261 taataataca ggtacttcca ttgtttctct taatgtcgat aactacggga gctattcctg
    49321 caatgttaac tggcgtaatg atcgcctgtc tgccggaaaa gatcgggtca cagaaacgtt
    49381 atcgttgtct ggttggcggc ataggtggag tcgttatcac cgagatctat tgcgctgtta
    49441 tcgtacatat taaggacatg gcttcctcag cgttatttga aaacattctt tctggtgaaa
    49501 atctcgttgt ccgcatcatc ccagcattgc tggcaggcgt ggtgatgagc agaatcatta
    49561 cacgtctacc cggattggat atttcatgtc ctgaaacaga ctctttaagc taagcggaat
    49621 gttttatcta tttgtcatta aatgttggag aacaggccgg agcgtaattc acacatccgg
    49681 ccttattttt aagcttaacc gagcgctaat tctgcggcag cggctttatc agcgattacc
    49741 atcagcgaag ggtgcagctg caacacggaa gctggaacat cttcggtaac cggaccttgc
    49801 agaacatttt tcagtgcctg cgctttacct gcgccactga caattatcag caagtttttc
    49861 gccgccatga tgcttttcgg ccccatcgtc acgtagctgt caggcactaa tgaaaagtcg
    49921 ccgcccaact cgccatgcgc cacaatatca accatctctc cctggatcgg gaactccacg
    49981 gtttgctcat ggaaatgggt cgtatttggc agattgccac aaaaatgacc atctacacct
    50041 aatcccaaca ccaccaaatc caggccgcct tcacgcgcca gtttctgatc atgctcgcgg
    50101 tagttatcaa tagtgagctt ctggatattc tcttctttga tccccgcagg ggtgaaaaac
    50161 agattacgca gattggtaat cgttacgcct tccccttctt taccgcgaaa tggaatttca
    50221 tcgaaattgt agaaatagca gttatcgtac cagggcttac ctttaaccag agtgatgaga
    50281 tattcgtaca tgcctttggg cgtgctaccg gcggtaattg ccaggttaac gcgacgcatc
    50341 tttgacatat aaccaagcag atgatgcgcc gcgacacggc tcatttcctg gtaatcttcg
    50401 gtaatgatta atttcatctt aatgccttat attatttgaa taatttctgt acgaaggctg
    50461 cataggcagg ccgccagaca tccatttcat ggttcagacc cggatattcc tggtaatcaa
    50521 acttaatttt tttctgctca agctcagttt tcagcccggc gatatccttg ccggttacaa
    50581 catctttatc cccaacaacc acagtaaaat tacgtagttg ctggttaata gctgccagct
    50641 cgttcagccg ggcagcgacc ccttcatctg gtacggtcgt cgtggtaaca ccactgaatg
    50701 tggccagcca gccaaagctt tccagatgat tcattcccga taccagtgcc tgatacccac
    50761 cttgtgaaag ccctgccagt gcgcgaccat cggcatcttt acggacatta aaacgcttgc
    50821 taatcagcgg gataatatcg ttcatcagtt cgcgatctgc cgctttagca tttaacggat
    50881 aaaagacttt acgtctctcc tgaggaacga aatcttcggg aataatgccc ttcgcatcgg
    50941 tttcggtatc cgggatcacc accaacatcg gtttaatttt cccttcagcc agcaggttat
    51001 ccatgatttg cgggatacgc ccctgatcaa tagcggaacg tccggtatca ccaaagccgt
    51061 gatagaaata gagcactggc aaaggctcgc ctatgccggt gtatcctggc ggggtccaga
    51121 catacatctg acgttcggat tgcaatgcgt tggagtggta agttatggcg atcaaatcac
    51181 catgcacaac agaacgtgta tccagataac tgcccggtac cagaatcata ctggagttaa
    51241 cctgacgctg aggcttagtc attgctgtac ctgtatcaat gctgcgtaca ccatcaacat
    51301 tgaaaaaata ctcgtacaga ttgcctttca ggacaggcgt gcgccacgac cagactcccg
    51361 cttcatcttt ggtcatcgga tgaatattgt ccggaaccgg aacacccacc actacggaga
    51421 cattttttgc cccaggggca aagtagcgaa aggtcacgct gttatcggta ttgacctgag
    51481 tcacatactg tttcaccgga atagtgggtg ccgggctggc tggcatatcc gctgctatgg
    51541 cccattgagc agaaataccg ctggcaatag ccagcgttaa agcagcaatt tttatattca
    51601 tcgtaatatt ccttttatta agttccatta ccaccagatt tcagcctggg caccgacagc
    51661 aaactggtcg tctttattgt cttcaaaggt gaagccatcc agttcgtttt ccagagcttt
    51721 gatatatgta gcgtagaaac gaatttccgg gcgcgaggtc aacatactgg tattgacttt
    51781 aaaggtatgg aagagcgtgg ttttataacc agactcatta tatttattgc tattcgcatc
    51841 tttgttttgc tgggtgaaat agcccagttc aacacctgtc tggttatatt ggtcccaaat
    51901 ataggccggg cgaacaaccg cacgaataga ttcgaaatca gaatgtgcgc ctgtttcgta
    51961 gctataaata tcgttaccga aggagtaaac aatcgcgtta gccacaatga aatgatcgcc
    52021 gatataagct tcgccctgcg aagtcagacg aactgcagtt ccgcctgtgt gatcaccata
    52081 ataacgacca ttaaaggtag taaatggact tgcaccagca taacgaccaa agttactggc
    52141 gatagagtta ttcgcaacca ggaaggagaa ttcgttgaag ccacccttat caaatttctg
    52201 cgttaaagat gtaccaaaca tccaggtatc tttccagtca taatacccgt tattatcctg
    52261 attatctttt tccgatgcgc tttcatttgc tgagacataa cgaccgctca ccattaaggt
    52321 ggctttatcc cataacggga tatctttata gcgtaaatca atggtattgg tattaatctg
    52381 ctgtttgttt tgcaggctgc gatcgtaatc atcgacatct tcacgaacca gcgcgatatc
    52441 aattttaccc ggaccgactt tccagttttc cagacctaca cctgcggcag catcggtacg
    52501 ctgcgttttc cagtcaagca tctggatttc aattttcggc gcaccgtgtt tacccaccca
    52561 gaaatcagcc tctggcgcaa agggcaggaa acctttggtg gtaacataca tatcagagaa
    52621 ctgcataaag ttctcgccac cggcgttatc gccaaaccag ccagtagagt actgctgacc
    52681 cacgttacca tccatcatca cgatggcatc aacccgtttg ccgttttcgt tgtagacacg
    52741 ttgttttaac tgcaaatcaa accagccgga gtattcgtta ccaaagcggc ccagagaacc
    52801 aattgcccat gatttaggtg aaccatgaga ggctgttccc caaccagaac ggtagtagcc
    52861 gttgtaacta aagccgattt catctttcac aaatttactg aaatctttca gagtcatgga
    52921 agaataaatc gagcctgtct cactggcatt tttctcttca tttttaacca gtacagcatc
    52981 aggctttgcg gcacgactgg tcgggaatgg attggcggca tacccttgat cattcgtact
    53041 tacgctacga ttcaccgtcg ctggcgtata ctttttcttc tcttcatctt tatatttttt
    53101 cagttcgctt tgcgtttctc ttaacgcctt ttctaatagc tcaaggcgtt gttccactgt
    53161 taatgattca gccaatgatt gtgcggaaaa ggctaacggt gccattaata agatggcaga
    53221 ggtaataata tttcgtctaa acataatatc cctttatggt gcaaagaaag aattaacgca
    53281 tcgcatccag actgttctga atgcgacgat atttaaggtg ctttattggt tattttttta
    53341 atgatagccc ccgagttttg atcacttctg cataccatcc gaagcttttc ttgcgcgttc
    53401 ttgtgaggct tccttcacca ttatcatcac gatccacata aataaagccg tagcgcttag
    53461 acatttgtga atgagatgca ctgactaaat caattggccc ccaactggtg taccccataa
    53521 tatccacacc atcggcaatc gcttcattta cctgtaccag gtgatcgttt aaataggcaa
    53581 ttcgataatc gtcctgtatc gaaccatcca cttcaacgct gtcttttgcg cctaatccgt
    53641 tctcgacaat aaataacggt ttttgataac gatcccaaag cgtatttaac agaatccgta
    53701 atccaaccgg atcaatttgc cacccccact ctgaactttt cagatgcgga tttgggatca
    53761 tattcagtat gttgccctgc gcatttttat taatgctttc gtcgtgggaa acacaaccgg
    53821 tcatgtaata actaaaagag atgaaatcta cggtatgttt taaatcttct gcgtcacttt
    53881 cagtcatctc aatggtgata ttgtggtcgc ggaagaaacg ctgcatatag ccgggatact
    53941 gaccacgcgc ctgaacatca ccaaagaaca tccagcgccg gttctcttcc atggcctgca
    54001 acatatcctg tggctggcag gtgagggagt aaaccagccc accgagaagc atattgccaa
    54061 ttttcgcttc ggggatcagg ctatgacagg ctttaactgc ccgcgcactg gcaaccagtt
    54121 gatgatggat cgcctgataa acttccgcct cgccactctc ttctgccagc cccacgcccg
    54181 tgaatggcgc gtgtaatgac atgttgattt cattaaatgt cagccataac gcaactttat
    54241 gttggtagcg ggtaaagacc gtgcgtgcgt aatgctcgaa gtgatcgatg accgcccgat
    54301 tagcccaacc gccgtagttt ttcaccagcc catatggcat ttcgtaatgg gataacgtta
    54361 ccagcggctt gatccccgcc tgcgccattt catcaaacag ccgctcgtaa aacgctaacc
    54421 ccgcttcatt cggttcggct tcatcgccct gaggaaaaat tcgcgcccag gcaatggaaa
    54481 tacgcagaca ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtgat
    54541 aaaaatcgat ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc
    54601 ccattacgcc atgaggctgt aaatctgagg tcgtgatccc tttgccatct tcctgccagg
    54661 cgccttccac ctgattggca gctgttgcac caccccaaag aaatgtttct ggaaatgctt
    54721 tcataattaa ctccttttat cgttagcgaa tgatggataa cagcggctca ccagcgttta
    54781 tctgcgccgt gccgtggggt aatacgtccg taaaatcatc gctattactg attaataccg
    54841 gcgtcgtcag atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat
    54901 cgcctgtatt gaccttgtca cccacgttga cgtgagcgga aaagaatttg ccgtccagtt
    54961 ttacggtgtc gataccgaca tgaatcagga tctccacgcc atcatctgac tcaatgccaa
    55021 tggcgtgtaa tgtggcgaac aacgaagcaa tttgccccgc caccggagaa cgcacttcac
    55081 caacagaggg cagaatggca atacctttac ccaacaggcc gctggcaaac gtggtatcag
    55141 cgacgtgaat gagcggcaca atatctcccg tcatcggtga acagataccg ccctgctcag
    55201 gtgtaataac ctctggtgtt ttctcttgct gggcaacttg cgctgactga ctcttagcgg
    55261 tgatgaaatg aaacatcacc gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa
    55321 taacgctggc ccatacggtg aaatcaatcc ccgttgacgg gatggtttgc atgaaggtga
    55381 aaatacctgg caaaccaaag gagtaaactt tcgtttgcgc gtagccaatg atggtggccc
    55441 ccaatgcccc acttatgcag gcgataacga aggggtactt acgcggcaga ttgacgccat
    55501 ataccgctgg ttcggtgata ccaaacaaac ctgtcaacgc cgctgatccc gccaccactt
    55561 ttttctgcgc atcgcgttcg cagaggaaga cgccgagcgc cgccccgact tgcgccataa
    55621 tggcgggcat taacagcggg atcatggtgt cgtagcccag cacggtgaag ttattgataa
    55681 ttaaaggtat caaaccccaa tgcagaccga acatcacaaa gatttgccag aaaccgccca
    55741 ttaccgcgcc tgcaaatgca ggaaccgcct gataaagcca gagataaccg gcagcaatca
    55801 gttcacttat ccaggttgat agcggcccca ccagcagaaa ggtgacgggt gtgataacca
    55861 tcagacatag caatggtgtg aagaaatttt tgattgccga aggtaaccac gcattaagtc
    55921 ggcgttccag aatgctgcac aaccaggcag aaaaaataat gggaataacc gatgacgagt
    55981 aattcaacaa tgtgaccgga atacccagga aatccaggcc cagcgcatcc actttttgcc
    56041 cgttctcgaa ggcagtcaga attaatggat gcactaacgc tccgccaatc accatggcag
    56101 taaatggatt gccgctgaag cgtttccctg cggtgtagcc caggattatc gggaagaacc
    56161 aaaacaaggc atcactggcg ctaaataaaa ttaaataagt accactttgt tcggtcgtcc
    56221 actgaaaagt gagtgccaga gccaacatac ctttcaggat cccggttgcc gccatcaaac
    56281 cgatcagagg cgtaaaaata cctgaaataa cataaacaaa gcggtttaac agattacctt
    56341 tatcatcatt ttccggtgcc tgttgcactt tttcgccaag gcctgccacg ctgttaaccg
    56401 ccaggaagac atcggccaca tggttaccta tgacaacctg aaactggcca ccgctttcca
    56461 ccaccataat aataccgggg gtctttttca gtacctcagc ttgcgctttg ctttcatcct
    56521 ttaacttaaa acgtaatcgc gttgcacaat gcatcagact cacaatgtta tctgcgcccc
    56581 cgactcctgc gactattgtt ctggctaact ccgtcataac ttgccctcta ccgctttgcg
    56641 gcaaaactcc aaaaaaaaac ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc
    56701 aggttttgcc cgcttagtgc ggtaacaatc ctttattcag taataatatt tcagtgttct
    56761 ttgcgcacgc gctctatatt tatggctaaa aacataatct ctgcaggtga gattttacgt
    56821 tgatactgca aaccaataaa aatggcgatc cgctccgcac attgccatgc ttgcgggtaa
    56881 ttttgcttta ctgcttgttg taatgattca tcactatcgt taatcgatgc atgttcaaga
    56941 atacgccagg ataaaaattt cagatgcgta accagtcgct gataactcaa gctttcttcc
    57001 tggtaattaa ggctgaactg aaattttatt aattgcagca tttctcgcat taattgcgtg
    57061 acacctgcaa catcctccat atttccgctc atttgggcac tgaccagatg catggcaata
    57121 aagcccactt catctttcgg taactgcacg cccaaccgtt tatcaataat ggttaacgct
    57181 tcttccccta gctggaactc tttcgggtaa agccgctgga tatcccacag cagcgggttg
    57241 ggcagtagca cgttttgctg aaagcgttta atcgcaaact ggcaatggtc agttagcgag
    57301 atataaatac tgtcctgcaa ctttcccagg cgctcctgcg ccagagagat aatacgatca
    57361 caggttgcca tcacctcaag aggcatatga cttaagagtt cgcttaatcg cccgttcagt
    57421 tcatgactgc tcaaggcata ctctttttct attccacttg agttaattct ttcgcctggg
    57481 cgtttttgaa agccaattcc gcgccccatg acgacttttt cccgctgttg atcatcaata
    57541 accaccacaa cattattgtt gagaattttg gtgatttgca tgttcatagc aaggaccttt
    57601 tttataaaca aaaaaacccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc
    57661 gaatgcagta acaatccagt cattaattaa tggtttttat aacgaacatc caggttcgaa
    57721 aattaattta gttgcgtgca gagaaaatag caatg
//
LOCUS       DADTFG010000010        50411 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.010, whole genome shotgun sequence.
ACCESSION   DADTFG010000010 DADTFG010000000
VERSION     DADTFG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.010"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            83..2509
                     /locus_tag="J1138_000646"
                     /pseudo
     CDS             83..2509
                     /locus_tag="J1138_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
     gene            2570..4993
                     /locus_tag="J1138_000647"
     CDS             2570..4993
                     /locus_tag="J1138_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBA8452489.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAQAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWLPMLENPVKTQISVFTWTDAIDHGTEMTAP
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FRVDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            5004..5621
                     /gene="dmsB"
                     /locus_tag="J1138_000648"
     CDS             5004..5621
                     /gene="dmsB"
                     /locus_tag="J1138_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBA8452490.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTNPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5623..6477
                     /locus_tag="J1138_000649"
     CDS             5623..6477
                     /locus_tag="J1138_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBA8452491.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNNADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGIIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6520..7134
                     /gene="dmsD"
                     /locus_tag="J1138_000650"
     CDS             6520..7134
                     /gene="dmsD"
                     /locus_tag="J1138_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBA8452492.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDSWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFAGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7294..8586
                     /gene="clcB"
                     /locus_tag="J1138_000651"
     CDS             7294..8586
                     /gene="clcB"
                     /locus_tag="J1138_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBA8452493.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRLTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(8539..9234)
                     /gene="bioD"
                     /locus_tag="J1138_000652"
     CDS             complement(8539..9234)
                     /gene="bioD"
                     /locus_tag="J1138_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBA8452494.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9359..10579)
                     /gene="mlc"
                     /locus_tag="J1138_000653"
                     /gene_synonym="dgsA"
     CDS             complement(9359..10579)
                     /gene="mlc"
                     /locus_tag="J1138_000653"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBA8452495.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYDDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMTGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10714..11607)
                     /locus_tag="J1138_000654"
     CDS             complement(10714..11607)
                     /locus_tag="J1138_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8452496.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11714..12967
                     /locus_tag="J1138_000655"
     CDS             11714..12967
                     /locus_tag="J1138_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452497.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            13365..13700
                     /locus_tag="J1138_000656"
     CDS             13365..13700
                     /locus_tag="J1138_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBA8452498.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            13793..13876
                     /locus_tag="J1138_000657"
     CDS             13793..13876
                     /locus_tag="J1138_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452499.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13976..14797
                     /locus_tag="J1138_000658"
     CDS             13976..14797
                     /locus_tag="J1138_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBA8452500.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14836..15165)
                     /gene="mdtI"
                     /locus_tag="J1138_000659"
     CDS             complement(14836..15165)
                     /gene="mdtI"
                     /locus_tag="J1138_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBA8452501.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRCKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15152..15517)
                     /gene="mdtJ"
                     /locus_tag="J1138_000660"
     CDS             complement(15152..15517)
                     /gene="mdtJ"
                     /locus_tag="J1138_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBA8452502.1"
                     /translation="MYIYWTLLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15929..16963
                     /gene="tqsA"
                     /locus_tag="J1138_000661"
     CDS             15929..16963
                     /gene="tqsA"
                     /locus_tag="J1138_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBA8452503.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16988..18376)
                     /gene="pntB"
                     /locus_tag="J1138_000662"
     CDS             complement(16988..18376)
                     /gene="pntB"
                     /locus_tag="J1138_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBA8452504.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18387..19919)
                     /gene="pntA"
                     /locus_tag="J1138_000663"
     CDS             complement(18387..19919)
                     /gene="pntA"
                     /locus_tag="J1138_000663"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBA8452505.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            20443..21387
                     /locus_tag="J1138_000664"
     CDS             20443..21387
                     /locus_tag="J1138_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA8452506.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVITG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKDAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21573..22955
                     /locus_tag="J1138_000665"
     CDS             21573..22955
                     /locus_tag="J1138_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA8452507.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAIMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARVRNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22992..23714
                     /gene="folM"
                     /locus_tag="J1138_000666"
     CDS             22992..23714
                     /gene="folM"
                     /locus_tag="J1138_000666"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBA8452508.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(23711..24046)
                     /locus_tag="J1138_000667"
     CDS             complement(23711..24046)
                     /locus_tag="J1138_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBA8452509.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24175..24894
                     /gene="rstA"
                     /locus_tag="J1138_000668"
     CDS             24175..24894
                     /gene="rstA"
                     /locus_tag="J1138_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBA8452510.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            24898..26199
                     /gene="rstB"
                     /locus_tag="J1138_000669"
     CDS             24898..26199
                     /gene="rstB"
                     /locus_tag="J1138_000669"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBA8452511.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26275..27204
                     /gene="tus"
                     /locus_tag="J1138_000670"
     CDS             26275..27204
                     /gene="tus"
                     /locus_tag="J1138_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBA8452512.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRENGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27201..28604)
                     /gene="fumC"
                     /locus_tag="J1138_000671"
     CDS             complement(27201..28604)
                     /gene="fumC"
                     /locus_tag="J1138_000671"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBA8452513.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(28747..30393)
                     /locus_tag="J1138_000672"
     CDS             complement(28747..30393)
                     /locus_tag="J1138_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBA8452514.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            30592..31767
                     /gene="manA"
                     /locus_tag="J1138_000673"
     CDS             30592..31767
                     /gene="manA"
                     /locus_tag="J1138_000673"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBA8452515.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            31868..33376
                     /locus_tag="J1138_000674"
     CDS             31868..33376
                     /locus_tag="J1138_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBA8452516.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTVNSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(33421..34707)
                     /gene="uidC"
                     /locus_tag="J1138_000675"
     CDS             complement(33421..34707)
                     /gene="uidC"
                     /locus_tag="J1138_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBA8452517.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMIGARADMST
                     RWYLDGDKSFGFALGAVKIKPSESSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPT
                     VSEGALGYWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLA
                     ASWHDDTSRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNT
                     SQPNETALVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQ
                     SWQLGVNYRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNF
                     FLAADKGREKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(34746..36119)
                     /gene="uidB"
                     /locus_tag="J1138_000676"
     CDS             complement(34746..36119)
                     /gene="uidB"
                     /locus_tag="J1138_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBA8452518.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDITN"
     gene            complement(36116..37927)
                     /gene="uidA"
                     /locus_tag="J1138_000677"
     CDS             complement(36116..37927)
                     /gene="uidA"
                     /locus_tag="J1138_000677"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBA8452519.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(38317..38907)
                     /locus_tag="J1138_000678"
     CDS             complement(38317..38907)
                     /locus_tag="J1138_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA8452520.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEVFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(39136..39903)
                     /gene="hdhA"
                     /locus_tag="J1138_000679"
     CDS             complement(39136..39903)
                     /gene="hdhA"
                     /locus_tag="J1138_000679"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBA8452521.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVNEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(40015..41043)
                     /locus_tag="J1138_000680"
     CDS             complement(40015..41043)
                     /locus_tag="J1138_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HBA8452522.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWVSTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAEK"
     gene            41140..42732
                     /locus_tag="J1138_000681"
     CDS             41140..42732
                     /locus_tag="J1138_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBA8452523.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            42742..43914
                     /gene="malY"
                     /locus_tag="J1138_000682"
     CDS             42742..43914
                     /gene="malY"
                     /locus_tag="J1138_000682"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBA8452524.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQPDGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            44018..45019
                     /gene="add"
                     /locus_tag="J1138_000683"
     CDS             44018..45019
                     /gene="add"
                     /locus_tag="J1138_000683"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBA8452525.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(45055..46095)
                     /locus_tag="J1138_000684"
     CDS             complement(45055..46095)
                     /locus_tag="J1138_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA8452526.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEIAYVESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            46338..46463
                     /gene="blr"
                     /locus_tag="J1138_000685"
     CDS             46338..46463
                     /gene="blr"
                     /locus_tag="J1138_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBA8452527.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            46736..46951
                     /gene="ydgT"
                     /locus_tag="J1138_000686"
     CDS             46736..46951
                     /gene="ydgT"
                     /locus_tag="J1138_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBA8452528.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            47037..47477
                     /locus_tag="J1138_000687"
     CDS             47037..47477
                     /locus_tag="J1138_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBA8452529.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            47554..48135
                     /gene="rsxA"
                     /locus_tag="J1138_000688"
     CDS             47554..48135
                     /gene="rsxA"
                     /locus_tag="J1138_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBA8452530.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            48135..48713
                     /gene="rsxB"
                     /locus_tag="J1138_000689"
     CDS             48135..48713
                     /gene="rsxB"
                     /locus_tag="J1138_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBA8452531.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            48706..>50411
                     /gene="rsxC"
                     /locus_tag="J1138_000690"
     CDS             48706..>50411
                     /gene="rsxC"
                     /locus_tag="J1138_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBA8452532.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KAR"
BASE COUNT        12293 a        13035 c        12417 g        12666 t
ORIGIN      
        1 tgggtagact ctctctattg ttagcgcgct aaatattcaa tatataaaca tttatataac
       61 gataaagaac agggagtgag ttatgtccaa aaatgaacaa atggtgggca tcagccgcag
      121 aacactcgtt aaatctaccg cgataggttc tctggcgctg gctgcaggcg gtttttcttt
      181 gccgtttacc ctgcgcagtg cagaagcagc ggtacaacag gccaacgaaa aagtggtctg
      241 gggtgcctgt tccgtcaact gtggtagccg ctgtgcactt cgtctgcatg ttaaagataa
      301 tgaagtgacc taggtggaaa ctgacaacac cggcagcgac gagtatggca accatcaggt
      361 acgcgcctgt ttgcgcggtc gctccatccg tcggcgtatt aatcatcccg atcgcctgaa
      421 ttacccaatg aaacgcgtgg gcaaacgcgg cgaaggcaaa ttcgaacgga ttagctggga
      481 tgaagccctg gatactatcg ccagtagcct gaaaaaaacc gtcaaacaat atggcaatga
      541 ggctgtatat attcagtact cttcggggat tgttggcggc aatatgaccc gctcttctcc
      601 atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc ggttcactaa atcagtatgg
      661 ctcctacagc actgcgcaaa tttcctgtgc catgccctac acctacggca gtaatgatgg
      721 caacagcacc acggatattg aaaacagcaa gctggtcgtg atgtttggta acaacccggc
      781 tgaaacccgc atgagcggcg gtggcatcac ttatcttctt gaaaaagcac gcgagaaatc
      841 gaacgcaaaa atgattgtta tcgatccgcg atataccgat acggctgctg gtcgtgaaga
      901 tgaatggctt cctattcgcc cgggcaccga tgccgcgctg gttgcgggta ttgcctgggt
      961 attgattaac gaaaatctcg ttgatcaacc ttttctcgat aaatactgcg tcggctatga
     1021 cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac tataaagcct atattcttgg
     1081 tgaaggcgat gataaaacag cgaaaacgcc gcagtgggct tcgcaaatta caggtatccc
     1141 ggtagaccgt atcatcaaac tggcgcgtga aattggcaca gcaaaacccg cttatatctg
     1201 ccagggctgg gggccgcaac gccaggcaaa cggcgagctg acggcacgcg ctattgctat
     1261 gttgcctatt ttgacgggca atgtcggcat cagcggcgga aatagtggtg cgcgtgaatc
     1321 gacctacacc attaccatcg aacgcttgcc ggtgctggat aatccggtca aaacgtcaat
     1381 ctcctgcttc agctggacag atgctatcga tcatggcccg caaatgacgg caatccgcga
     1441 cggcgtccgg ggcaaagata aactagatgt gcccattaag ttcatctgga actacgcggg
     1501 aaataccctg gttaatcagc attctgacat caacaaaacg catgaaattc tgcaggacga
     1561 atcgaaatgc gaaatgattg tggtcatcga aaactttatg acctcatcgg caaaatatgc
     1621 cgacattctg ctgccagacc tgatgaccgt tgagcaggaa gatattattc ctaacgacta
     1681 cgccggtaac atgggatatc tcattttcct ccagcctgtc accagcgaaa aattcgaacg
     1741 caaaccaatt tactggatcc tgagtgaagt cgcgaaacgt cttggacctg acgtctatca
     1801 aaagttcact gaaggtcgca cgcaggaaca atggttacaa catctgtacg ccaaaatgct
     1861 tgccaaagat ccggcgttac cgtcttacga cgaattaaaa aaaatgggta tctataagcg
     1921 taaagatccc aatggtcatt ttgtcgccta caaagcattt cgtgacgacc ccgaggcaaa
     1981 tccacttaaa acgccttccg gtaagattga aatttattcc agcaagctgg cggaaattgc
     2041 ccgtacctgg gaactggaaa aagatgaagt gataagccca ctgccggttt atgcctcaac
     2101 ctttgaaggc tgggactccc ctgaacgtag tgccttccca ctgcaactgt ttggtttcca
     2161 ttacaaatcc cgtacgcact cgacctacgg caacattgat gtcctgaagg cagcctgccg
     2221 tcaggaagtg tggatcaacc ctattgacgc acagaaacgt gggattgcca acggcgatat
     2281 ggtgcgggtg tttaaccatc gtggcgaagt tcggctacca gccaaagtga caccacgcat
     2341 tctccctggg gttagcgcta tgggccaggg agcctggcac gaggccaata tgtctggcga
     2401 taaaatcgac cacggcggct gtgtgaatac gctaaccact ctgcgccctt caccactggc
     2461 gaagggaaac ccgcagcaca ctaatctggt cgagatcgaa aaaatataac ccactacaac
     2521 cataaattct ggcatgacat tttgtttgaa aagcaataag tgagtaatga tgaaaatcca
     2581 taccacagag gcgctcatga aggctgaaat cagccgtaga agtctgatga aaacctccgc
     2641 acttggcagt ctcgcgctgg caagcagtgc tttcactttg ccgttttccc aaatggtccg
     2701 agcggcacag gcaccggtag aagagaaagc ggtctggagt tcctgcaccg ttaactgcgg
     2761 gagccgctgt ctgttacgtt tgcatgtgaa agatgacacc gtgtactggg tggagtctga
     2821 tacgacaggt gacgacgtct acggtaatca tcaggttcga gcgtgtttac gcgggcgctc
     2881 tatccgtcga cggatgaatc atcctgatag gttgaaatat cccatgaagc gcgtcggcaa
     2941 gcgcggtgaa ggtaaatttg aacggataag ttgggatgaa gccctggata ccatcagtga
     3001 taatcttcgg cggatcctaa aagattacgg caatgaggct gtacatgtcc tgtacggaac
     3061 aggcgtagat ggcggaaaca tcaccaactc gaacgtcccg taccgtctga tgaactcttg
     3121 cggtggtttt ctcagtcgct atggtagcta cagtaccgca cagatcagtg ccgcgatgag
     3181 ttatatgttc ggtgccaatg atggcaacag cccggatgat atcgccaata cgaaactggt
     3241 cgttatgttc ggaaataacc cggcagaaac gcggatgagc ggcggtggtg tcacttacta
     3301 cgtcgagcaa gcccgcgaac gttcaaatgc acgcatgatc gtcatcgatc cacgttataa
     3361 cgacacagct gccggacgtg aagatgaatg gctgcccatt cgccctggca ccgatggcgc
     3421 actggcctgt gcgattgcct gggtactgat tactgaaaac atggtcgatc agccatttct
     3481 cgacaaatat tgtgttggtt acgatgagaa aacgctgccc gccaacgcac cacgtaacgc
     3541 gcattataaa gcctatattc tgggcgaagg gcctgacggc atagctaaaa cgccggaatg
     3601 ggcagcaaaa atcaccagca taccggcaga aaaaattatc cagttggcac gagagatcgg
     3661 ttcagcaaaa cctgcttata tttgtcaggg ttgggggcca caacgacact ccaacggcga
     3721 acaaacgtcc cgcgctattg ccatgctttc cgttctcacc ggtaacgtcg gcataaacgg
     3781 cggcaactca ggtgtacgcg aaggtagctg ggatctgggg gtagaatggt taccgatgct
     3841 cgagaatcct gttaaaacgc agatttccgt ctttacatgg acagatgcta tcgaccatgg
     3901 tacggaaatg accgcgcccc gtgatggtgt tcgtggaaaa gaaaaactgg atgtccccat
     3961 caagttttta tggtgctacg ccagtaacac attgatcaat caacatggcg acatcaatca
     4021 cacccatgag gtgcttcagg atgacagcaa gtgcgagatg attgttggca ttgaccactt
     4081 catgactgcc tcggctaagt attgcgatat cctgttaccc gacctgatgc caacagagca
     4141 agaagacctc atctctcatg agtctgccgg gaatatgggc tatgtgatcc tcgcccaacc
     4201 cgcaacctca gcaaaatttg aacgcaaacc catctactgg atgctgagtg aagtcgccaa
     4261 acgcttagga ccagacgttt atcaaacctt tactgaaggt cgcagtcagc atgaatggat
     4321 caaatatctc catgcgaaaa cgaaggaacg taaccctgag atgcccgact acgaggagat
     4381 gaaaacgacc gggatcttta agaaaaaatg cccggaagaa cactacgtcg ctttccgcgc
     4441 attccgtgta gatccacagg caaacccatt gaaaacacct tcgggaaaaa tcgaaattta
     4501 ttctgaacga ctggcgaaga ttgcggatac ctgggaattg aaaaaagatg aaattattca
     4561 tcctcttcca gcttatacgc caggttttga tggctgggac gatcccctgc ggaaaactta
     4621 tccactgcag ttaacgggct tccactacaa agcgcgtacc cactccagtt acggcaatat
     4681 tgatgtatta cagcaggcct gcccacaaga ggtgtggatc aaccccattg atgctcaggc
     4741 acgcggtatc cgtcatggcg ataccgtgcg ggtatttaac aataatggag aaatgctgat
     4801 tgccgcaaaa gtgactccgc gtattctgcc tggcgttacc gccatcgggc aaggtgcttg
     4861 gcttaaagcg gatatgtttg gtgaccgggt cgatcacggc ggcagtatca atattctgac
     4921 ctctcaccgc ccttcaccgc tggcaaaggg aaacccgtcg cacagcaatc ttgtccagat
     4981 cgaaaaggtt taaggagtag cccatgacca cacaatatgg attttttatc gattccagcc
     5041 gctgtactgg ctgtaaaacc tgcgaactgg cgtgcaaaga ttttaaagat cttggcccgg
     5101 aagtcagttt ccgccgtatt tatgaatatg ctggcggcga ctggcaggaa gataatggcg
     5161 tctggcacca gaacgtgttc gcttattatc tctccatctc ctgtaaccat tgcgatgacc
     5221 ccgcatgtac aaaagtctgc cccagcggtg caatgcataa gcgtgaagat ggtttcgtgg
     5281 tggttgatga agatgtttgt atcggctgcc gctactgcca catggcttgt ccgtacggcg
     5341 cgccacagta caatgctgaa aaagggcaca tgacgaagtg cgatggttgt tattcgcgcg
     5401 tcgccgaggg gaaacaaccc atatgtgtcg aatcctgccc actgcgtgca cttgagttcg
     5461 gtccaattga agaattgcgc cagaagcacg gcactctggc tgccgtcgcg ccactgccgc
     5521 gcgcgcattt cacaaacccc aatatcgtta tcaaacctaa cgccaacagc cgcccgaccg
     5581 gcgataccac tggttatctg gctaatccgg aggaagtgta aaatgggaaa tggatggcat
     5641 gaatggccac tggttatctt taccgtattg ggtcaatgcg tagtaggcgc gctgattgtt
     5701 agcggaattg gctggttcgc cgccaaaaat aacgcagacc gtcagcacat tattcgtggc
     5761 atgtttttcc tctggctgtt gatgggcatc gggtttattg cctctgtcat gcacctggga
     5821 tcgccactgc gggcgtttaa ctcgctgaat agaatcggag cttctggtct gagtaacgag
     5881 attgctgccg gttccatatt ttttgccgtt ggcggattat ggtggctggt ggcggtcatc
     5941 ggtaaaatgc cgcaagcgtt ggggaaactc tggttacttg tcagcatggc gctcggcatc
     6001 attttcgtct ggatgatgac ctgcgtgtat caaatcgaca ctgtgccaac ctggcataac
     6061 ggttacacta cgctggcttt cttcctgacg gtattgttga gcggtccaat acttgctgcc
     6121 acaattctac gtgcagcccg cgttacattt aataccacac catttgccat cattagcatt
     6181 ctggcgctga tcgcttgtgc cgggttgatt gtgctgcaag gtttgtctct ggcgtctatt
     6241 cactcatccg tacaacaagc cagcgcactg gtaccagatt acgcctcctt acaggtctgg
     6301 cgtgtggtat tgttatgcgc aggtctagga tgctggcttt gtccgctcat tcgtcgccgt
     6361 gaacctcacg ttgccggact tgttctgggg ttaatcctca ttcttggcgg tgagatgatc
     6421 gggcgtgtgc tcttttatgg cctgcacatg acggtaggaa tggctattgc aggttaacaa
     6481 attggtgcgg ggcaacccgc acatttcagg atgttaggaa tgacccattt ttcacagcaa
     6541 gataattttt ctgtcgcggc gcgcgtgttg ggcgcgctgt tttattacgc tccagagagt
     6601 gcggaagccg cacctctggt tgcggtactc accaatgaca gctgggaaac tcagtggcct
     6661 ctaccagaag cgtcattagc gccgctggtg acggcttttc agacacagag tgaagagact
     6721 cacgcccagg cctggcagcg tttgtttgcc ggcccgtggg cactgccgtc tccgccgtgg
     6781 ggatcggtct ggctggatcg cgaatctgtg ctgtttggcg attcaacatt ggcacttcgt
     6841 cagtggatgc gcgagaaagg cattcagttc gaaatgaagc aaaacgaacc ggaagatcat
     6901 tttggttcac tgctgctgat ggctgcgtgg ttggcggaga atggtcgcca gacagaatgc
     6961 gaagaattgc tggcatggca cctttttccg tggtcaacac gttttcttga cgtttttatc
     7021 gaaaaagcag aacacccttt ctaccgtgca ctgggtgaac tcgctcgctt aacgctggcg
     7081 caatggcaat cacaactgtt gattcctgtc gcggttaaac cgctgtttcg ataggatcac
     7141 cgtattattg ccggatggtg acgattacac gcaattcggc aattaatagg ttacagtgtc
     7201 acgttttttt atctcttaaa gcacgcactg cttttgcggc tggcctcttt tgccgcaaaa
     7261 tagtcgcccg tgtttcattg cccatttctg ctcatgcatc atctacacat ctatccggat
     7321 ctgcgcacta tgttccgccg tctgcttatc gcaacaatcg tcggtattct tgcggccttt
     7381 gccgttgccg ggtttcgtca tgcgatgctg ctactggagt ggctgttcct caataatgac
     7441 tccggcagtc tggtcaatgc agcgacaaat ctttccccgt ggcgacggct gctaaccccg
     7501 gcgctcggcg gactggcggc gggtttgttg ctgatgggct ggcagaaatt tacccagcaa
     7561 cgccctcatg cgccgaccga ttacatggaa gcgttgcaaa ccgatggtca gtttgattac
     7621 gcagcaagcc tggttaaatc gcttgcctct ctactggtag taaccagcgg cagtgcaatt
     7681 ggtcgcgaag gtgcgatgat tcttttagct gcccttgcag cctcgtgttt tgcccaacgt
     7741 cttacgccac gccaggagtg gaaattatgg atcgcctgtg gagctgcggc gggaatggct
     7801 gcggcctatc gcgccccgct tgctggcagt ttatttatag ccgaagtgct gtttggcact
     7861 atgatgttgg cctctctcgg cccggtgatt atttccgccg tcgtggcgtt gctggttagc
     7921 aatcttatta atcatagcga cgcattactc tacagcgtac aactctcagt gacggttcag
     7981 gctcgtgact atgcgctgat tatcagtaca ggtgtgctgg caggtctgtg cggaccactg
     8041 ttgttaacgt taatgaacgc ctgtcatcgt ggatttgtga gtctcaagct tgcgccgccc
     8101 tggcaactgg cactgggcgg gttgatcgtg ggtctgcttt ccctgttcac acctgcagtg
     8161 tggggcaacg gctatagcac cgtacaatcc tttttaaccg ctccgccact gttaatgatc
     8221 attgccggga tcttcctctg taaactgtgt gccgtgctgg cgagtagtgg ttctggcgcg
     8281 cccggtggtg tctttacacc gacgctattt atcggtcttg ccattggcat gttgtatggt
     8341 cgtagcctgg gattatggtt acctgatggc gaagaaatta cgcttttact cggattgaca
     8401 gggatggcga cactgttggc ggcgaccacg cacgcgccga ttatgtcgac gttgatgata
     8461 tgtgaaatga ccggggagta tcagctactc cccggtttat tgattgcctg cgtaattgcg
     8521 tcggtaattt cgcggacgtt acaccgtgac tctatctacc gccagcacac tgcgaagcat
     8581 agctaagcgg atgtattgcc ccagttcacg ctgttcggcg cgcggcagat aaggcagttc
     8641 accaatgagc ggtgccggaa gttttttacc gagcacatca atgatttccg cataatgcgc
     8701 cagtcctggg ttgattcggt tagccaccca gccaatgagc ggcagcccgt cgttggcaat
     8761 cgcctgagct gttagcagtg catggttaat gcaaccttcc tgaataccga caaccatcaa
     8821 caccggcagt tgttcctgca ccacccattc agagagcgga cgcaaatcat tcatcagact
     8881 gcgccagccg ccagtccctt ctaccacgac atgatcgact ttatcggtca ggtttgccag
     8941 gccgtttgaa atgagggtgt aattgattgg gcaactgtgc gccacgctac tttcttcttc
     9001 gcttaacgcg ataggattaa ctgcttcata aggcagttcg atggttgaaa cactctgcaa
     9061 caccagggca tctttattac gcagcccttc gggtgtctct ttgctcccct tcgctacggg
     9121 tttatatccc gcaaccgttt ttccctggga ggctaacgct tgtagcaatg cgcgggaaac
     9181 caccgttttc cctacagaag tgtctgtacc ggtaataaag aaacgcttca gcatcactaa
     9241 ctccaccgtt atgcttcaca aatataaacc aggaaaataa ttaaccttga aagtctaagt
     9301 tatgctttcc tggcccaaat tgagatagcg caaattttgg tagaacagtt aaaaaatgtt
     9361 aaccctgcaa cagacgaatc aacaaagaac cgttatacat cgcgtctttt accagcgcag
     9421 cgcctgtcat cgtgccctgg ttagaaaact gagtactctc aacgctaatg tgctgactat
     9481 acgcaggaag ggcctgctga cggatgctgt ccgagataac tgggaagagg atatctgccg
     9541 ctttacttaa cggtgagcca atcagtattt tttgtgggtt aaataaattc accatgatgg
     9601 caagaatgcg accgacatgc gcgcctaccc cggtaatgat gtcttttgcc agtagatcgc
     9661 cgcgcaatgc cgcctgacac aatgagtcca cggttaacgg ttgtccatgt aacatcgagc
     9721 tcatggattg attgagacgc agctgtgcca gctcaagaat actgtccaca ctggcgatgg
     9781 tttcgaggca accgtgattc ccgcaataac agcgtttccc atacgggtcg acctgtgtgt
     9841 ggcctatttc cacgaggcta ctgctgcctg cgtgtagcag atgaccatcg gtaatgacgc
     9901 cagcccccac gttgtgatcg ataaccacct gaatcacatc gcgcgccccg cgtgaggcac
     9961 caaacaaggc ctcagccatc gtccatgcgc tgatatcatg ctgaatataa accggtacgc
    10021 cggtatgctg ctccagcgcc tcgccgagcg gcatctcttt tacatcatcg tagaacggca
    10081 tgcgatgtac aataccattt tccgtatcaa taattcccgg caaggttatg gcaatcgaag
    10141 tcagacgctc aagttttttc tggtggcgga taaaaaactg atcgatatgg gaaatgatac
    10201 gatccagcaa tggcgagtca tcttttaacg ccagttcctg cgactcctcc actaccagtt
    10261 tgctgctcag atcgcgcaaa gcaaggaaaa tctccccgcg actaatgcgc agagaaagat
    10321 agtgccaggc ttcagtttca accaccagcc ccactgccgg acggccacgg ttccccgctt
    10381 ctttgatttc cagctcttgc accaggtgtg cttcgagcat ctcacggaca attttagtga
    10441 tactggcagg agccagttgc gccagacggg aaagatcgat acgcgagact ggaccaagct
    10501 gatcaatcag gcgataaacc gcgcccgcgt tggtctgctt tatttgatca atgtgcccag
    10561 gctgattttc agcaaccacc gcatactccc tatattttcg cgctccgaaa taatctgtag
    10621 gctatggtga agcacttcaa tacgtgtcgt caaattttta cttaggcatg tgattaacag
    10681 cacatttttc gggctttttc gctgaaattt ccctcacctg agagcattca gcagatgaat
    10741 acgaaagtta cgcgcagccg gactttgttc atgatgtttc ggccagacca accacatttc
    10801 agaaaccgca tcctcttcag caatcggcac ccagcgcatt tcgttgagct gaacacgttt
    10861 aaatgacgca ggcaaaattg aaacacccag accagcggaa accagaccga tgatggtcat
    10921 tgcctcaccc acctcctgag tgatgacggg cgtcaaattg taacgtctca tcagcccgag
    10981 aatatcgtca tacagccctg ttccgacgtg cggatcaaaa aagacaaagg gttctttcgc
    11041 cagttcagcc agcgttacac tcgggttatt tgccagggga tgatcgtgcg ggatcatcgc
    11101 cataagcggt tcatggacga tgactgcgtg ttcaagcgtc tccggtaacg ctgtgttacg
    11161 cagcaatccc atatccagcg ttccttcaat gagcggagcg atttgctcgc gagtgttcat
    11221 ttcgcgggtt tgtaaatgga catcgggata atcacggcga aacagcgata acgtatcgga
    11281 caccgcccga ataaaaggag cggacgaagt aaaaccaatg cgcaactccc ccgcttcgcc
    11341 ctgatgcagc ctttcagcgc gagcggcagc atcatccacc atagacagga tttgccgact
    11401 atctacaaga aactgttttc ctgcttccgt cagcaataca ctgcgattgg tccgtgccag
    11461 cagtcgggca ccaatttgtt gctccagcgc ctgaatctgc tgacttagcg gtggttgcga
    11521 aatattcagg cgggcagcgg cacgcccgaa atgcagctct tctgcaacag cgacaaagta
    11581 acgcagatga cgaagttcaa tattcatatt taaaacatct tatttgagat tattaatata
    11641 ttagacagaa caattcgatt ttcctaatct attcataagc ctgatccaca ggcatattta
    11701 gcaaggattt caagtgagcc gtactacaac tgttgatggc gctccggcaa gcgacactga
    11761 caagcaaagc atttctcagc caaatcaatt tattaaacgc ggtacgccgc aatttatgcg
    11821 cgtcaccctg gcgcttttct ctgccggact ggcaactttc gcacttctct attgtgtgca
    11881 acctatcctt ccggtgcttt cacaagagtt tggcttaacg cccgcgaaca gtagtatttc
    11941 actgtccatt tcaacggcga tgttggctat cggtttgctg tttactggcc cgctatccga
    12001 tgccattggt cgcaaaccag tgatggtcac ggcgctactg ttggcctcca tttgtacgtt
    12061 actttcgaca atgatgacca gctggcacgg cattttgatt atgcgcgcct tgattgggct
    12121 ttcgttaagt ggcgtggcag ccgttggcat gacttatctt agcgaggaaa tccaccccag
    12181 tttcgtggcc ttttcaatgg ggttgtatat cagcggcaac tcaattggcg gcatgagcgg
    12241 acgcttaatt agcggtgtct tcacggactt tttcaactgg cgaattgctc tggcggcaat
    12301 cggttgtttc gcgttggcct cggcgttgat gttctggaaa atcctccctg aatcacgcca
    12361 ttttcgcccg acttcgctgc gccctaagac gttattcatc aactttcgtc tgcactggcg
    12421 tgaccgggga ttaccgttat tgttcgcaga aggctttttg ctgatggggt cgttcgtcac
    12481 gctgtttaat tacatcggct ataggttgat gctttccccc tggcatgtca gtcaggccgt
    12541 ggttggctta ttatcgctgg cttatttgac cggtacatgg agctcaccca aagccggaac
    12601 catgaccacc cgctatggac gcggtccggt gatgttgttt tcgacggggg ttatgctgtt
    12661 tggtttactg atgaccttat tcagctcgct gtggctgatc tttgccggaa tgttactctt
    12721 ctcagccgga ttcttcgcag cccactccgt tgccagcagc tggatcggcc cccgcgcaaa
    12781 acgcgctaaa ggccaggcct cctcgctgta tctgttcagt tactatctgg ggtcgagtat
    12841 tgccgggacg ctgggtggtg ttttctggca taactatggc tggaacggcg tcggcgcatt
    12901 tattgctctg atgctggtca ttgctctgct ggtcgggacg cgtttgcatc atcgtctgca
    12961 cgcctgaaaa aataagtccg gactgaggta aatacccgtc cggacttatt gcctgctcaa
    13021 accaacgtta atagccatcc taaaatagac gaagcgccag ccaattcccg cagcgcatct
    13081 agcgtcatca ggattataag tacccaaata aacggattca ttttgctgtg tgtcatttat
    13141 tactgatgcg cagttattct accgctttgt aagtagtaaa atagttaaca cgatcaagac
    13201 tactattatt ggtagctaaa ttttccttaa gtcacaatac gttattatca acgctgtaat
    13261 ttattcagcg tttgtacata tcgttacacg ctgaaaccaa ccactcacgg aagtctgcca
    13321 ttcccaggga tatagttatt tcaacggccc cgcagtgggg ttaaatgaaa aaacaaattg
    13381 agggtatgac aatgaaaaaa gtattagctc tggttgttgc cgctgctatg ggtctgtctt
    13441 ctgctgcctt tgctgcagag actgcgacca cacctgctcc gactgcgacg accaccaaag
    13501 cagcgcctgc gaaaaccaca catcataaaa aacagcataa agcagcacct gcccagaaag
    13561 cgcaggcggc taaaaagcat cataaaaatg ccaaagctga acagaaagcc cctgaacaaa
    13621 aagcgcaggc ggcgaagaaa cacgccaaga aacacagcca tcagcaaccg gcaaaacctg
    13681 ctgcacaacc cgcagcgtaa gttttcaaca gtaatgctgg cgcgccccct cgcgcctgaa
    13741 aattacggtg ctaagcgggt aacgtttagc accgccttta gcggagggta gtatggtggg
    13801 ccgttatcgc tttgagttca ttcttatcat ccttatttta tgcgcactga ttgccgcccg
    13861 tttttatctt tcctgatcgt agttatctga ttttactccc actttcatcc cgtcccgtct
    13921 atagtattta tgagggtttg cttttaataa tcataattac ccaccagagc gtgatatgcg
    13981 tacaaccatt gctgtagtgt tgggtgcaat tagtttgacg tctgcttttg tgtttgcaga
    14041 taaaccagac gttgccaaat cggcaaacga tgaggtcagc accctgtttt ttggtcatga
    14101 tgatcgtgtg ccagtgaatg acacgaccca atcaccgtgg gatgcggttg gccaactgga
    14161 aacggccagc ggcaatttat gtacggcgac actgattgca cccaatctgg cattaacggc
    14221 aggacactgt ttattgacgc cgccaaaggg taaagccgat aaagcggtag cgctgcgttt
    14281 cgtgtcaaat aaaggtcttt ggcgctatga tatccacgac atagaaggcc gcgttgatcc
    14341 gacactggga aagcggttaa aagcagatgg ggatggctgg attgtacctc ccgcagctgc
    14401 gccctgggac ttcggtttga ttgtgctacg taatccccct tctggcatta caccgttgcc
    14461 gttatttgag ggagataaag ccgcgcttac cgccgcatta aaagcggctg gtcgtaaagt
    14521 gactcaggcg ggctaccctg aagatcatct cgatacgttg tacagtcatc aaaactgtga
    14581 agtgactggc tgggcgcaaa cgtcggtgat gtcgcatcag tgcgatacct tgccgggtga
    14641 cagcggttcg cctctgatgt tgcataccga tgacggctgg caattaattg gggtgcaaag
    14701 ctcggctcct gccgcgaaag atcgctggcg cgccgataac cgggccattt ctgttaccgg
    14761 ttttcgcgac aagctggatc aactgtcgca aaaataatgt tcaaacgctg cccgacagcg
    14821 cgggcagcgt gttcatcagg caagtttcac catgatcatt ccagccaaca gcagcaccag
    14881 gccaatccag cctttacgat ttaaccgttg accaaacaag atccaacctg cggctaacgt
    14941 ggcggcaata ccaaacccgc cccacaatgc ataagcgaca gacaagtcga tccctttaac
    15001 ggcctgagaa agcgcactaa aggcagccag caccgccgcc agggaaagca agccaaatat
    15061 tttgcaacga aagccgtcag aaaatttcaa aaagacgtta gcaacgattt ccagcacgat
    15121 tgccaatgcc agccaggcgg cgtgaaccca ttcaaactgc gccatggttc acctccagtt
    15181 caggtttacg cgctttacgg gtacctgatt tgatcaatac aatcccggcg accagggtgg
    15241 ttaacccggc aattttcatc agcgataaac tttcgtcgaa taacaaaacg ctaaacaagg
    15301 taataaataa aataccgata ccttcccaca gcgcataagc cacgcctaag gcgatttttt
    15361 taacggcgaa agagagaaat atatacgaca gagaaatcat caccagcatt aaaataaagc
    15421 cgccatttcc ctcactgacg ctcgcccatt tcatggacag cgtaccggta atttctgtag
    15481 caatagccag acctaataaa gtccaataaa tatacattgt ccttctcctg caagagaatt
    15541 attttaattt tcgcttaatt cagcgaaacc aaagtaaagt ggtcaagccc cgcgcttagc
    15601 gcaagctttg gcagaagaaa ggactaaagc gcgttgcgcc agtgctgctc acctacgagc
    15661 aagatagtgg atgaggtacg aagatgggta aatgtagaaa ataacgtcct gaacaaattg
    15721 tccataatat tacaattatc cgcagtgttg cttctcgtcc tcgcggatga taattgtcct
    15781 cggtagttga acacgcctga tttgtatcat agcttaagaa ttaactcaaa atattttcac
    15841 ttctttacct gagcggtttg attttcgtta tgatgacgga gcgaaaaaga cattattatt
    15901 agcaaaggaa gaaaaaacgg ggacaagcat ggcaaagccg atcatcacgc tcaatggcct
    15961 aaaaatcgtc attatgttgg gaatgctggt cattattctc tgcggtatcc gttttgccgc
    16021 cgagatcatc gtgccgttta ttctcgcatt atttattgct gttattctta acccgctggt
    16081 gcaacacatg gtccgctggc gtgtgccgcg tgtactggca gtgtcaattt tgatgaccat
    16141 catcgtgatg gcgatggtgt tgctgttagc ttatctgggt tccgcgctca atgagttgac
    16201 gcggacgtta ccgcaatatc gcaactctat tatgacgccg ctgcaagcga ttgaaccgtt
    16261 gttgcaacgc gtagggattg atgtctcagt tgaccagctg gcgcattaca ttgatccgaa
    16321 cgcggcgatg acgttgctca ccaacttatt gacgcagtta tctaatgcca tgtcatcaat
    16381 ctttttattg ctgctgacgg tgctgtttat gttgctcgaa gtgccacaat tgcccggaaa
    16441 atttcagcaa atgatggcgc gtccggttga agggatggcg gcgattcagc gtgcgattga
    16501 cagtgtgtct cattatctgg tgctgaaaac agccatcagc atcatcaccg gcctggtcgc
    16561 ctgggcgatg ctcgccgcac tcgatgttcg cttcgctttt gtctggggat tgctggcctt
    16621 tgcgcttaat tacatcccga atattggttc agttctcgcg gcaatccccc ctatcgctca
    16681 ggtactggtg tttaatggct tctacgaagc gttgctggtg ctggcgggat atctgctgat
    16741 taatctggtc ttcggcaata ttctggagcc gcgcatcatg gggcgtgggc tggggctttc
    16801 cacattggtg gtatttttgt cgttgatttt ttggggatgg ttgttaggac cggtgggtat
    16861 gctgctttcc gtgccgttga caattattgt caaaattgcg cttgaacaaa cagcgggagg
    16921 tcaaagcatc gccgttctgt taagcgatct caataaagag tgacggcctc cgcagaggcc
    16981 gtcagggtta cagagccttc aggattgcat ccacgctggc tttggcgtca ccaaacagca
    17041 tgtgggtgtt ttccttgaag aacagcgggt tttgcacacc agcatagcca gtgttcatcg
    17101 aacgtttaaa gacaatcacg ttctgcgctt tccacacttc cagcacaggc ataccagcaa
    17161 tcggactctt cggatcatcc tgcgccgccg gattaaccgt gtcgttagca ccaatcacca
    17221 gtacggtatc ggtatcagca aagtcatcgt tgatctcgtc catttccagc acgatgtcat
    17281 acggtacttt tgcttcagcc agcaatacgt tcatatgtcc aggcaaacgc cccgcaaccg
    17341 ggtggatacc gaaacgtaca ttaataccac gagcgcgcaa tttctcagta atttcagcga
    17401 caggatattg cgcctgcgca actgccatgc cgtaccccgg agtaatgatc actgaatggg
    17461 agtttttcag cagttccgct gtctcttctg cggtgatttc gcggtgctcg cccacttcct
    17521 gatcatcacc agtagaagag ccgtcagtgc cgaaaccacc cgcaataacg ctgataaagg
    17581 aacggttcat cgccttacac ataatgtaag aaaggatagc ccccgaagaa ccgaccagcg
    17641 caccggtcac aatcagcagg tcgttgctaa gcataaagcc cgcagccgca gccgcccagc
    17701 cggagtacga gttcagcatc gacaccacca ctggcatatc tgcaccaccg atggaggcga
    17761 ctaaatgcca gccgaatacc agcgcaatgg cggtcattat cagcaatgcc agcacttgca
    17821 ggccgacgct gtccgtgcga acaaatacaa tcagcagcag gaaggaaacg accagagccg
    17881 ccaggttcat tttgtgacgg tttggcagca tcaacggttt agacgaaatc ttgccacaca
    17941 gtttgccgaa cgccaccacc gaacccgtga acgttaccgc cccgatgaag ataccgagga
    18001 acacttccgt caggtgaata ttgaccagaa tcggtgccat tcccgcgtca tgatgcagat
    18061 agctgttaaa gccaaccagc actgccgcca gacccacgaa gctatgcagg atcgccacca
    18121 gttctggcat ttcggtcatt tcaacttttt tcgccagccg gataccaatt gccccaccaa
    18181 tgaccatagc cagcaagatc cagccaacat tacccgtatc cggtccaaaa atggttgcga
    18241 ttaacgcaat cgccatcccg gcgataccga agttgttacc ctggcgagac gtttcatgtt
    18301 tcgaaagacc ggccagactg aagataaaca ggatcgcggc aacaatgtat gcagctgtaa
    18361 ctaatcctcc agacatatgt taccccttaa tttttgcgga acattttcag catgcgctga
    18421 gtcacggtga agccaccgaa aatattaatg ctggctataa gcaccgcgat aaaactaagg
    18481 aagctaaccc agccgccctg gccaatctgc aacagtgctc cgacaacaat aatccctgaa
    18541 atcgcgttgg tgaccgacat caacggtgta tgcagcgcgt gcgatacatt ccacaccacg
    18601 taataaccga caacgcaggc cagcgcgaac acagtgaagt gtccaaggaa ctcttttggc
    18661 gcaacgcttg ccatccagcc gaaaaggatg attgccaacg ccatcaacgc gtatttacgc
    18721 cacggtgagc aggcacattt ttcctcagtt ttcacttccg gtgccgcttt ttgtgccgcc
    18781 tgcggctgag ctgatacctg aatcggcggt gcaggccagg taatttcgcc cgcacggatc
    18841 acggtcacgc cacgaatcac cacatcatca aaatccacag tgatgttgcc gtctttctct
    18901 ttgcacaaca gtttcagcag attaacgagg ttagtgccgt aaagctgtga ggattgcgtc
    18961 ggcagacggc ccggaagatc ggtgtaacca attactttga caccattttc cgtagtgaag
    19021 atttcacccg gcacggtgta ttcacagttg ccgccgtttt gggctgccag gtcgacaatc
    19081 acactgcccg ccttcatgga gtcaaccatt tcacgggtaa ttagcttcgg cgctggtttg
    19141 cctggaataa gcgcggtggt gacaatgata tcgacctctt ttgcctgggc ggcaaagagt
    19201 tccatttccg ctttgatgaa cgcgtccgac atcactttgg catagccatc gccgctgccc
    19261 gcttcctctt taaaatccag ctcgaggaat tctgcgccca tactttgaac ttgttctttc
    19321 acttccgggc gggtgtcgaa tgcacgcaca atcgcgccga gactgtttgc tgcgccaatg
    19381 gcggccagac ctgcgacacc ggcaccaatc accatcactt ttgccggtgg cactttcccg
    19441 gccgcagtga tttgtccggt aaagaagcgc ccaaattcat gtgccgcttc aacaatggcg
    19501 cgataaccgg cgatattcgc catcgagctt agtgcgtcca gcgattgtgc gcgtgagata
    19561 cgcggcacag agtccatcgc catcacggtc acgttacgtt ccgcaagttt ttgcattaat
    19621 tccggattct gcgcaggcca gataaaactc accagcgttg tcccaggatt cagtaacgca
    19681 atttcatcat ctaacggcgc attgaccttc agaatgatct ctgactgcca gacgctattc
    19741 ccttctacaa tttcagcgcc cgcttgcaca aacgctttat cgtcaaaact tgccagttga
    19801 cccgcgccgc tctctaccgc gacggtaaaa cccagtttca gcagctgttc cactgttttt
    19861 ggcgttgctg caacacgggt ttcattggtt aaccgttctc ttggtattcc aattcgcatg
    19921 atattccctt ccatcggttt tattgatgat ggtttgcttg tgtcaggagc cacacaagcc
    19981 gctcatgtac gagctaaatg ttactccgtt aaaataaatt agtaacaaac gccctataac
    20041 gtactgaaaa ttatgcctgt gatctagcgc caaaaatcag tatttcggcg tgaactcgca
    20101 aaatattaac gattcagccg tgatagtggg ataaacacct tagaacgccg gataaagact
    20161 gataattgtc ttcggcggcc gggtaaaacg agacatcgcc ccggcacaaa tcactactta
    20221 acattaaatt aacttataca attcagttgc ttcagtagta ttgatggtga tacggctgtt
    20281 ttttaagcat agacggtcat ttgagcagaa ttaaaattgg tttaaggaat gtgatatgaa
    20341 aaatgacgca gacagttaca ccgtttaaat gcaataatca gccacgtttc tcgttaataa
    20401 caataccagt acctggtttg cgcaaggcga aggattattt ttatgaagct taagaacacc
    20461 ctcctggcgt cggcactgct ttctgctacg gcattctccg ttaacgcagc aacagaactg
    20521 acaccggagc aagcggcagc ggttaaacct tttgaccgtg tagtgattac cggtcgtttt
    20581 aatgctattg gcgaagcggt gaaagccgtt tctcgtcgcg cagataaaga aggtgccgcc
    20641 tctttttatg ttgtcgacac ttctgatttt ggtaacagcg gtaactggcg tgtggtcgct
    20701 gacctctata aagccgatgc tgaaaaagca gaagaaacaa gtaatcgcgt aattaacggt
    20761 gttgtcgaac tgccgaaaga tcaggctgtt ctgattgaac cgtttgacac ggtcaccgtc
    20821 cagggcttct atcgtagcca gccagaagtc aatgatgcca tcaccaaagc ggcaaaagcg
    20881 aaagatgcct actctttcta cattgttcgt caaatcgatg ccaaccaggg tggtaaccag
    20941 cgtattactg cattcatcta taaaaaagat gctaagaaac gtatcgtcca gagcccggat
    21001 gtaatcccgg cagattccga agcaggacgt gcagctctgg ctgccggtgg cgaagccgcg
    21061 aagaaagttg agatcccggg tgttgcgact accgcatcac caagttctga agtcggtcgc
    21121 ttctttgaaa cccagtcatc aaaaggcggg cgttacaccg tcacgctccc ggatggcact
    21181 aaagtcgaag aactgaacaa agcgacagca gcgatgatgg tcccgttcga cagcatcaaa
    21241 ttctctggca actacggcaa catgaccgaa gtctcctatc aggttgcgaa acgtgccgcg
    21301 aagaaaggtg ctaagtatta ccacatcacc cgccagtggc aggaacgtgg taataacctg
    21361 accgtcagcg cagatctgta taaataacta ttcagttact cctataaggc ggcttgatgc
    21421 cgcctttcgc atttttagtc ataaatttcc ctgcgaccat tgcatgcaga cacatctctt
    21481 cgtaaaatcc cgcgcttcag tggcaaactc catttttatg cgttttgccc taatatttat
    21541 tcttcttgtc acgttttaat cactggatat cgatggaaaa gaaactggga ctgagcgcac
    21601 tcaccgcgct ggtattaagc tcaatgctgg gcgcgggtgt tttcagtctg ccgcaaaata
    21661 tggcggcagt tgccagcccg gcagcactgc tcatcggctg gggtattact ggcgctggca
    21721 ttttattgct ggcctttgcc atgctgatcc tcacgcgcat tcgccccgaa cttgacggcg
    21781 gtatctttac ctatgcccgc gaagggtttg gcgagctaat cggcttttgt tccgcatggg
    21841 gatactggct gtgcgcggtc atcgccaacg tctcctatct ggtgatcgtt ttttccgcgt
    21901 taagcttttt tacggacacg ccggaattgc gcctgtttgg cgatggtaat acctggcaat
    21961 cgatcgtcgg tgcatcggct ttattgtgga ttgttcactt tttgatcctg cgcggggtgc
    22021 aaaccgctgc cagcattaac cttgtggcga cactggcaaa attgttgccg ttgggtctgt
    22081 ttgttgtgct ggcgataatg atgttcaaac tggatacctt caagctcgac ttcaccggac
    22141 ttgcacttgg cgtacccgtg tgggaacagg tgaaaaacac catgctgatc accctgtggg
    22201 ttttcattgg tgtggaaggt gccgtggttg tttctgcgcg tgtgcgtaat aagcgtgatg
    22261 ttggcaaagc gacactgctg gcggttctct ccgctctggg cgtttatttg ctggtaacgc
    22321 tgctttcgct gggcgtggta gcacgtcctg aactggctga aattcgtaac ccgtcaatgg
    22381 ccggtctgat ggtggaaatg atgggaccgt ggggcgaaat catcatcgct gccggtttga
    22441 tcgtttccgt ttgcggcgcg tacttaagct ggaccatcat ggcagcagaa gttccgttcc
    22501 ttgcagccac tcataaagca ttcccacgca tttttgcgcg ccagaacgca caagctgcgc
    22561 catccgcttc actgtggctg accaacatct gcgtgcaaat ttgtctggtg ctcatctggc
    22621 tgaccggttc cgattacaac acattgctga ccatcgcctc agaaatgatt ctggtgccct
    22681 atttcctggt cggcgcgttc ttgctgaaaa tcgcaacccg tccactgcat aaggcggtag
    22741 gtgtcggtgc ctgcatttat ggcttatggt tactgtacgc ctctggtcca atgcacttgc
    22801 tgttatccgt tgttctgtat gcgccggggc tgctggtttt cctgtacgcg cgtaaaacgc
    22861 atacacatga taatgtactg aaccgtcagg aaatggtgct gattggcatg ctgttaattg
    22921 cctcagttcc ggcgacctgg atgctggtgg gataacgttc atcccatcgt tggatagcaa
    22981 aggagatcac gatgggtaaa acccagccct tgccaatatt aattactggc ggaggtcgcc
    23041 gcatcggcct cgccctcgca tggcatttca ttaatcaaaa gcaaccggtg attgtcagct
    23101 atcggacgca ctatcccgcc attgatggac tgattaaagc aggtgcgcag tgtattcagg
    23161 ctgatttttc gaccaacgac ggtgtgatgg cgtttgccga tgaagtacta aaaagcaccc
    23221 atggtctgcg tgctattttg cataacgcca gcgcgtggat ggcggaaaaa ccgggtgcgc
    23281 cactgaccga cgtactggct tgcatgatgc agatccacgt taatacccca tacctgctca
    23341 accatgctct ggaaagatta ctgcgtgggc acggacacgc cgccagcgat atcattcact
    23401 ttaccgatta tgtggtggag cgcggtagcg acaaacatat tgcctatgct gcaagcaaag
    23461 cggcactgga taatatgacc cgctcgtttg cccgcaagct ggcaccggaa gtgaaagtga
    23521 attctatcgc gccatcgctg atcctgttta atgaacatga tgatgccgaa tatcgacaac
    23581 aggcgctgaa taaatcactg atgaaaaccg cgcctggcga gaaagaagtg atcgacctgg
    23641 tcgattactt acttaccagc tgctttgtca ccggacgcag tctcccactt gatggcggtc
    23701 gtcatctgcg ttaatgcagc ttaatccagc atataatcaa cacccatgcg cttattcccc
    23761 agcacgcaac agagccaaca aaagcagcgg gcaagcgcat cattccggtg aaataccata
    23821 gggacgccag gtaaacaaaa tagggaataa tcgaccacat actaaaaatg atggttgcgc
    23881 gtaaggcttc aatgccgcgt tcgctggcaa caatataatg cgcgataagc gcaaaggtcg
    23941 ggaaaagtgg aatcagcccg gcgatataat aatttttcgt ttttgctaaa acaccaatca
    24001 acagcactac cagcgcaccg agcgcggctt tgattaccag ccccatcttt ttaccttaac
    24061 acttccataa caagtcatca gtagaatacc tgatgaaacc ttgtttagaa acgattgata
    24121 gtaagtaaaa acagtgcggt gtattgtgac gtttttatat ctaccgtgaa tgttatgaac
    24181 actatcgtat ttgtggaaga tgatgcggaa gtcggtgcac tgattgccgc atacctggca
    24241 aaacatgata tgcaggttac cgtagagccg cgcggcgacc gggccgaaga aactattctg
    24301 cgagaaaatc cggatttggt gttactcgac atcatgctac caggcaagga cggcatgacc
    24361 atttgtcgtg atttacgcgc aaagtggtcc ggaccgattg ttcttctaac ctctctcgat
    24421 agcgatatga accacatcct ggcactggaa atgggcgcct gcgactatat tctcaaaacg
    24481 acgccccctg ctgttttgct agcgcgttta cgcttgcatt tgcgtcagaa tgagcaagcc
    24541 acagtgacca aaggtattca ggaaacgtct ctgactccct acaaagccct gcatttcggc
    24601 acgttgacca tcgatcccat caaccgcgta gtctccctgg ctaacactga aatctcgctc
    24661 tcgacagctg atttcgaatt attgtgggaa ttagctaccc atgccgggca aatcatggac
    24721 cgcgatgcat tgctgaaaaa tttacgcggc gtcagttatg acggactgga tcgtagcgtg
    24781 gacgtggcta tttcgcggtt aagaaaaaaa ctgctcgata acgccgcaga accttatcgt
    24841 attaaaaccg tgcgtaacaa aggctatctt tttgcgcctc atgcgtggga ataagcgatg
    24901 aaaaaactgt ttatccagtt ttacctgtta ttgtttgtct gcttccttgt gatgtctctg
    24961 ctggtcgggc tggtgtacaa atttaccgcc gaacgcgcag gcaaacagtc gctggatgat
    25021 ttgatgaaca gttcgctgta tctgatgcgc agcgaattgc gtgagatccc cccacacgac
    25081 tggggtaaga cgctgaaaga gatggattta aatctctctt tcgatctgcg tgtcgagcca
    25141 ctgagtaaat accatcttga tgatatttcc atgcaccggc tgcgtggcgg cgaaattgtc
    25201 gctctggacg atcagtacac ttttttgcaa cgtatcccgc gcagccacta cgtgctggca
    25261 gttggtcctg ttccttatct ttattacctc catcagatgc gattgctgga tatcgccctg
    25321 atcgctttta ttgccatttc cctcgccttt ccggtgttta tctggatgcg tccgcactgg
    25381 caggatatgt taaaactgga agcagcggcg caacgatttg gcgatgggca tctcagtgag
    25441 cgtatccact ttgatgaagg ttcgagcttt gaacgacttg gcgtcgcatt taaccagatg
    25501 gcggacaata tcaacgcctt gattgccagc aaaaaacagc ttattgacgg tatcgctcac
    25561 gaactgcgaa caccgttagt gcgcctgcgt tatcgactgg agatgagcga taacctgagc
    25621 gccgccgaat cccaggcgtt gaatcgcgat atcagtcaac ttgaagcttt aattgaagag
    25681 ctgctgactt atgcccgact cgatcgccca caaaatgagc ttcatcttag cgagccagac
    25741 ctgccgttgt ggctgtcaac gcatctggca gatattcagg cagtaacgcc cgataaaacg
    25801 gtacggataa aaacgctcat gcaaggccat tatgcggcgt tggatatgcg cttaatggag
    25861 cgcgtgctgg ataatttgct caataacgcc ctgcgctact gtcactcaac ggttgaaacc
    25921 agcctgctac tgtcggggaa tagagcgaca ttaattgttg aggatgatgg cccagggatt
    25981 gccccagaaa accgcgaaca tatctttgaa ccttttgttc gcctcgatcc cagccgggat
    26041 cgctcaaccg gcggctgcgg gctggggctg gccattgtcc actctatagc actggcaatg
    26101 ggcggtacgg ttaattgtga caccagcgaa ctgggtggtg cccgcttctc gttcagctgg
    26161 ccgctatggc ataacatccc gcaatttacc tctgcctgac actatgcgca cggtggtcaa
    26221 gtcaccacca ttgtgctata aaataaggat gttgtaacta aagtggttaa tattatggcg
    26281 cgttacgatc tcgtagaccg actcaacact acttttcgcc agatggaaca agagctggct
    26341 gcatttgccg ctcatcttga gcaacacaag ctattggttg cccgcgtgtt ctctttgccg
    26401 gaggtaaaaa aagaggatga gcataatccg cttaatcgta ttgaggtaaa acaacatctc
    26461 ggcaacgacg cgcaatcgct ggcgttgcgt cattttcgcc atttatttat tcaacaacag
    26521 tccgaaaatc gcagcagcaa ggccgctgtc cgtctgcctg gcgtgttgtg ttaccaggtc
    26581 gataactttt cgcaagcagc gttagtcagt catattcagc acatcaataa actcaagacc
    26641 acgttcgagc atatcgtcac ggttgaatca gaactcccct ccgcggcacg ttttgaatgg
    26701 gtgcatcgtc atttgccggg gctgattaca cttaatgctt accgcacgct caccgttctg
    26761 cacgaccccg ccactttacg ttttggctgg gctaataaac atatcattaa aaatttgcat
    26821 cgtgatgaag tcctggcaca gctggaaaaa agcctgaaat caccacgcag tgtcgcaccg
    26881 tggacgcgcg aggagtggca aagaaaactg gagcgagaat atcaggatat cgctgccctg
    26941 ccacagaacg cgaagttaaa aatcaaacgt ccggtgaagg tgcagccgat tgcccgcgtc
    27001 tggtacaaag gagatcaaaa acaagtacaa cacgcctgcc ctacgccgct gattgcactg
    27061 attaataggg aaaatggcgc gggcgtgccg gacgttggtg agttgttaaa ttacgatgcc
    27121 gacaatgtgc agcaccgtta taaaccacag gcgcagccgc ttcgtttaat cattccacgg
    27181 ctgcacctgt atgttgcaga ttaacccccg gctttcatac tgccgaccat ctgttctggc
    27241 cgtacccagc tgtcaaactc ggcttcgcta agatacccca gcgcaagggc cgcagctttt
    27301 aaggtcagcc cttctttatg cgcttttttg gcgatctcgg cggctttgtc ataaccaatg
    27361 tgggtgttaa gcgcagtcac cagcatcagc gattcattga gtaattgatt gattcgctca
    27421 cgattcggtt caatgcccac tgcgcagtgt ttgttgaaac tttccatgcc atctgccagc
    27481 aagcgcaccg attgcaggaa attatggatc accatcggac ggaagacgtt cagttcaaag
    27541 ttaccggaag cgccacccat gttgatcgcc acatcgttcc ccatcacctg acagcagagc
    27601 atggttaatg cttcacactg cgtcgggttc actttccccg gcatgattga gctacccggc
    27661 tcattttccg ggattgagat ttcaccaatt ccgcagcgcg ggccagaggc cagccagcgg
    27721 acatcattgg caattttcat cagtgacgca gccaaccctt tcaatgcacc gtgcgcctga
    27781 accagggcat cacaggtcgc cagcgcttca aatttgttcg gcgcggtaac aaacggtgca
    27841 caggtaatga ctgccagttc atctgctacg cgacgcgcat attccggatg ggtatttagt
    27901 ccagtaccca ccgctgtacc gcccagagcc agttccgcta cgtgaggcag gctgtattcg
    27961 atatgtttga gattatgctc cagcatcgct acccagccgg aaatctcctg ccccagcgtt
    28021 aacggcgtcg cgtcctgcaa gtgggtacga ccgattttga cgatatcggc aaatgcgcgg
    28081 gatttttcac tcagcgtctg ggtcagggtt ttaagttgtg gaatgagttg cttgcgcagc
    28141 gccagcagcg ccgcaacgtg catcgcagtc ggaaagacat cgttggaact ttggcttttg
    28201 ttcacgtcgt cgttagggtg aactttacgt tccatcccgc gcacgccacc gagtaattca
    28261 ctggcccggt tagccagcac ttcgttcatg ttcatgttac tttgcgtgcc ggagccggtc
    28321 tgccagatag ccagcgggaa ttcgtcgtca tgctgtcctg ccagtacttc atccgccgcc
    28381 tgccgaatgg cgctcgcttt ctcttcagac aacaagccta aatcttcatt aacttttgcc
    28441 gctgcgcgct tggttagcgc cagcgcatga atcagtgagg tgggcatttt ctccgtcgaa
    28501 atgcggaaat gctccagcga gcgctgagtt tgtgcgcccc acagcttatc tgccgggaca
    28561 tcaatcgctc ccatcgaatc tttttcgctg cgtactgtat tcatggcctg ctcctcacct
    28621 gattaatttt ttctttctgt tttgctttcg ttaagcaact tttatcttac tcattattta
    28681 ccatttgata acaaatgttt ggtctttcgt gccatgtaaa aaaaccgccc cgaagggcgg
    28741 ctctgtttat ttcacacagc gggtgcattg tgtgagttgt atctgctgga agaagtcatt
    28801 tcctttatca tccacaagga taaacgccgg gaaatcttcc acttcaattt tccagatggc
    28861 ttccattccc agttccggat attcaacaca ttccaggctc ttaatacttc cctgcgccaa
    28921 tacagctgcc ggaccaccga tactgccgag gtagaagccg ccgtgttttt tacaggcatc
    28981 cgtcacctgc tggctgcggt tgcctttcgc cagcatgatc atacttccgc cctgcgcttg
    29041 cagttgatcg acataagaat ccatccgtcc ggcggtcgtt gggccaagag aaccggaggc
    29101 ataaccttcc ggcgttttgg ccggacccgc gtagtaaatc ggatgatctt taatgtactg
    29161 cggcagccct tcaccgttat ccatccgctc tttcagtttg gcgtgagcaa tatcacgacc
    29221 gacgataatc gtgccgttaa gcgataagcg tgtagaaacg ggatactgcg acaactgtgc
    29281 gaggatctct ttcatcggac ggttaaggtc aacgcgcacc gcttcgcctt ctcctgcttt
    29341 gcgcagctct tccgggatat atttgcctgg attatgttcc agtttttcga tccagatccc
    29401 ctgacggttg atcttcgctt tgatattacg gtcagcagaa caggagacgc ccataccgac
    29461 cgggcaggat gcgccgtgac gtggcaggcg aatcacgcgg atgtcatgag cgaagtattt
    29521 accaccaaac tgcgcaccca gaccaagatt ttgcgcttcg atcagcaatt ctttttccag
    29581 ttccacatcg cggaacgcct gaccgtgctc attcccttcc gttggcagtt catcatagta
    29641 tttagcggaa gcgagtttca ccgttttaag attcgtttct gcagaagttc caccaataac
    29701 gaacgcaata tgatacggag gacaggccgc cgtacccagc gtgcgcatct tctcaaccag
    29761 gtaatttttc agttttcccg gcgtcagtaa cgctttggtt tcctgataga gatacgtctt
    29821 gtttgccgaa ccgccacctt tggcgataca gaggaatttg tactcgtcgc catcaacggc
    29881 ataaagatcg atctgcgctg gcaagttggt gccggtattc acctctttat acatatccag
    29941 cggcgcgttt tgcgagtagc gcagattatc ctcgatataa gtgttataaa caccgcgcgc
    30001 cagcgctgct tcatcaccgc caccggtcca tacacgctgc ccttttttac caacaataat
    30061 cgcggtgccg gtatcctgac aggttggcag aacgcctttt gccgcgatgt cggagttacg
    30121 caggaattgc agcgccacat atttatcatt ttcgctggcc tccgggtcac gcagaatgtc
    30181 ggccacctgt tgttggtgag ccggacgcag cataaatgac gcatcatgaa acgcctgacg
    30241 cgccaacaga gttaacgctt cgggtgcgac tttcaaaatc tcctgccctt caaattcaga
    30301 tacgctaacg tgttcgctgg ttagcaggta atactcagta tcatcttttt tgagtggaaa
    30361 aggagcctga taatgaaagg gtttgtttga cattgttctc tcacttactg cctggtttgg
    30421 ttatgctctg ggcgggtgtt ccgttgccct gttaaaagcg agtaacaata tcctacacac
    30481 ttttttaaca aaaactgaga ctagtacgac tttttgcggc tccaggttac ttcccgtagg
    30541 attcttgctt taatagcggg attaatttcc acattaaaac agggattgat catgcaaaaa
    30601 ctcattaact cagtgcaaaa ctatgcctgg ggcagcaaaa cggcgttgac tgaactttat
    30661 ggtatggaaa atccgtccag ccagccgatg gccgagttgt ggatgggcgc gcatccaaag
    30721 agcagttcac gtgtgcacga tgccgcagga gacatcgttt cactgcgtga tgtgattgag
    30781 agtgataaag cgactctttt gggagatgcc gttgccaaac gctttggcga gttgcctttc
    30841 ctgttcaaag tattatgcgc agcccagccg ctctccattc aggttcatcc aaacaaacgc
    30901 aattctgaaa tcggttttgc caaagaaaat gctgcaggta ttccgatgga tgccgctgag
    30961 cgtaactata aagatcctaa tcacaagcct gagctggttt tcgccctgac ccctttcctc
    31021 gcgatgaacg cgtttcgtga gttttccgag attgtctccc tgctccagcc agtcgcaggt
    31081 gcacatccgg cgattgctca ctttttacaa cagcctgatg ccgaacgttt aagcgaactg
    31141 ttcgccagcc tgttgaatat gcagggtgaa gaaaaatccc gcgcactggc gattttaaaa
    31201 tcggccctcg acagccagca gggtgaacca tggcaaacga ttcgtttaat ttctgaattt
    31261 tacccggaag acagcggcct gttctccccg ctgttgctga atgtggtgaa attgaatcct
    31321 ggcgaagcga tgttcctgtt cgctgaaaca ccgcacgctt acctgcaagg cgtggcgctg
    31381 gaagtgatgg cgaactccga taacgtgctg cgtgcaggtc taacgcctaa atacattgat
    31441 attccggaac tggttgccaa cgtgaagttc gaagccaaac cggctaacca gttgttgacc
    31501 cagccggtga aacaaggtgc agaactggac ttcccgattc ctgtggatga ttttgccttc
    31561 tcgttgcatg accttagtga taaagaaacc accatcagcc agcagagtgc cgccattttg
    31621 ttctgcgtcg aaggcgatgc aacgctgtgt aaaggttctc agcagttaca gcttaaaccg
    31681 ggtgaatcag cgtttattgc cgccaacgaa tcaccggtga ctgtcaaagg ccacggccgt
    31741 ttagcgcgtg tttacaacaa gctgtaagag cttactgaaa aaattaacat ctcttgctaa
    31801 gcttattaaa ggcttataac accttcaggc ggccagtccg cctgatttca ttttatggat
    31861 aatcattatg aataaatcgc tggtagcggt aggcgtcatt gttgcgctag gcgtagtctg
    31921 gacaggcggc gcatggtata caggcaagaa gattgaaacc catctcgaag acatggtcgc
    31981 gcaggcgaac gcgcaactca aactgacagc tcctgaatcc aacctggaag tcagttatca
    32041 aaactatcat cgcggcgtat tcagcagcca gttgcaactg ttggtgaaac ccattgccgg
    32101 aaaagtaaat ccgtggatta aaagcggtca gagcgtcatc ttcaacgaat cggttgatca
    32161 tggtcccttc ccgcttgccc agcttaaaaa actgaacctg atcccgtcga tggcatcaat
    32221 tcaaaccacg ctggttaata acgaagtaag caaaccactg tttgatatgg caaaaggtga
    32281 aacgcctttt gagattaact cccgcattgg ttacagcgga gattccagtt ccgacatttc
    32341 gctcaagcca ctgaattacg agcaaaagga tgaaaaagtc gcatttagcg gcggcgagtt
    32401 ccagttaaat gcagacagag acggcaaagc tatctccctt tccggggagg cgcaaagtgg
    32461 tcggatagac gcggttaacg aatacaacca gaaagtgcag ttaaccttta ataatctgaa
    32521 aaccgacggt tccagcacgc tggcaagttt tggtgagcgc gtaggaaacc aaaaactgtc
    32581 gctggaaaaa atgaccattt cagtggaggg caaagaactg gcactgctgg aaggcatgga
    32641 gatcagcggt aaatcggatc tggtcaatga cggtaaaacg gtcaatagcc aactggatta
    32701 ctcgctaaac agcctgaagg tacagaatca ggatctgggc agtggcaagc tgactttaaa
    32761 agtcggccaa attgatggcg aagcctggca tcagtttagc cagcaatata acgcacaaac
    32821 tcaggcgctg ctggcacagc cagaaattgc caacaatccc gaactttatc aggagaaagt
    32881 gacggaagcc ttctttagcg ctctgccgct gatgctgaaa ggcgatccgg tgattactat
    32941 cgcgccgcta agctggaaaa acagtcaggg tgaaagtgcg ctgaatctgt cgctgttcct
    33001 gaaagatccg gcaacgacta aagaagcgcc gcaaacgctg gcgcaggaag tggaccgctc
    33061 ggttaaatct ctggatgcga aactgaccat tccggtggat atggcaactg agttgatgac
    33121 tcaggtagcg aagctggaag gttatcagga agatcaagcg aaaaaactgg cgaaacagca
    33181 agttgaaggt gcatcagcaa tggggcagat gttccgtctg accaccttgc aggacaatac
    33241 catcaccacc agcctgcaat atgctaacgg tcagataacg ttaaacgggc agaaaatgcc
    33301 actggaagat ttcgtgggta tgtttgcgat gccggcatta aacgttccgg ctgtacccgc
    33361 tattccgcag cagtaattca cagccaaaac gccggagatt tttctctccg gcgttttttc
    33421 ttacttcagc ataaagtcat actgaatact cattttcaca tcccagtaat tcaggcgatc
    33481 gcccagcgta ctgccatcgc gcttttcccg ccctttatcg gcagcaagaa agaaattcat
    33541 cccttgtaac ggcccttcgc tgacccgata gttcataccc cctaagatac ccgcgccttc
    33601 aatccgcaca tctcgtttac tggattcata gccgcgggtc acaatcggtg caaatgtcag
    33661 cgtaaattgc ggagttaaac gatagttgac gcccaattgc caagactgca ttccttcacg
    33721 gttacgatca agacttaatg aaaagggata gccaatgtcg gtatccacca cggcaccatt
    33781 cattgcatgg cgcaaataac caccactgcc aaatacgctt ccccacggcg cattccaggt
    33841 caattgtcca ctaaccaacg ccgtttcatt gggctggctg gtattacgac ttaaaccttc
    33901 aagttgcgcg taaaagccga gcaactcccc tttcaacgta taattcgggt caagcggtat
    33961 gcttttctcg agaatacttt gtatctgatt ggaaacatct ttctgtaccg atgcccccag
    34021 agaatagcga ctggtatcat catgccaact ggcggctaaa aaagaacgtg cgcgatgttt
    34081 atcgtatttt cccgtctggc tgttatacac cccttcatca cgaataccgg ttgttgaatc
    34141 ggtccataaa aatactcgtg aacgccacat tccccacagg tcaatatttg gcgttaaagc
    34201 ccaataacct aatgctcctt cagaaactgt cggtaatgta cgttgcgaac tgccagccat
    34261 taacggaata cgataaggta agctaccata actataatcc gtcccgaagc gacctaattt
    34321 aagcagacta ctttcactgg gttttatttt tactgccccc agggcaaagc caaaactttt
    34381 atcaccatca agataccacc gggtactcat atcagcacga gcgcctatca tatcagcacg
    34441 agcgcctaat ttataaacat aatacgcccc gccttcaacg ccaataatat tggaataata
    34501 accactattg aaatcgagca atcctccctg tacccatgcg taaatatctc ttcccgtgcc
    34561 agcactgggc ttatcagccc tgcgtaattc atttttcagt ctgatgcgta gtcccgcttc
    34621 atcgtcagcc ggttgcgccg cgtaagcaga ggtaaggcca gaggcagccg tcaggcaaat
    34681 aacggccatg gccactattt ttctcatagt tgcacctttg atgttctgat tatttttatt
    34741 gaatattaat tagtgatatc gctgattaat tgctgctgca tttttttacg attatcaatt
    34801 tcaaccacga tttctttgaa ttttttatcc gtgagcggat aaaaccagat aatgacgaat
    34861 gccagcagca taaatccgca aggtactaag gcaattgatg tgcgaatgcc cataataact
    34921 tccggcgttt gcacctgatt ggcaatatat ccgcttaatc ccaaaataaa ggcaggaatt
    34981 gaacctccga ttgcctgacc gcatttacgg gtaaatgaga atagtgaata ggtgagccct
    35041 tctattcgca cgccggtcag gtattcaccg tattctacag tatcagcttc cagtgcccac
    35101 atcacggtca tggtaacgcc ctgaccaatt gaagcgatgg ccaacgcaac caacgccacc
    35161 ggcagtgacc agaccgaaac ccaaaagaac agcagataac cgcaggttcc cagcaaagcg
    35221 ccaatcagga aggtattctt tttaccgatc ctcgcgacca tccccggcac cagcggtgcc
    35281 gatgccacag taccgaccag gttttgtacc agtaccagca cagtgaacag cccggtatca
    35341 tttaacacat agcgcacgta gaacaacgac gaggcgctga ccgcgaaggt cgaaatcagc
    35401 acacacagcg caccgatgca caacataaac agcgggcgat tccgtttcag ggtttgcaga
    35461 ctgatcttca atgacggctg cgccacgata cgtaccacat tctcacgcgt cgatttgaag
    35521 cagatgaagt aaagcaccat tccggcaatc gccagcacaa ttgtccagaa atgatatacc
    35581 gacaccatct cttccgggct ggagttctta atgctcggtc ctatcagaaa tgccaggcag
    35641 acaaaagtca atgaagcggc aatcccgcga gccgcgccca gacgggcgcg ggattgtggt
    35701 tgttgggtca tcgcggtagc aagtgaacca taaggaatat tcaccaggct gtagcaaagc
    35761 ccgagtccca tgtaggtcaa atatgcatac acaactttgc tgctatggct ccagtcggtc
    35821 ggcacccaga ataccagcac gctaaagatc attaacggcg cagtaccgaa gagtaaaaac
    35881 gggcggaatt ttccccatcg ggtattcaca ctgtccacca ctcgtccggc aaagacatcg
    35941 gcgaaggcat cgaatacccg caccagtaac agcatggtgc ccgccgcagc ggcaccgacg
    36001 ccagcgacgt cggtgtagta actcaacagg aagagcgccc ccattgcgaa ggcgaagtta
    36061 ttggcgacgt caccgaggct gtagccgacg atggtgcgcc aggagagttg ttgattcatt
    36121 gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc ggtccagcgt ttttgcagca
    36181 gaaaagccgc cgacttcggt ttgcggtcgc gggtgaagat ccctttcttg ttaccgccaa
    36241 cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt ccatacctgc tcaccgacga
    36301 cagcgctgac gcgatcaaag acgcggtgat acatatccag ccaggcgcac tgatactctt
    36361 cactccacat gtcggtgtac attgagtgca ggccggctaa cgtatccacg ccgtattcgg
    36421 tgatgataat cggctgatgc agtttctcct gccaggccag aagttctttt tccagtacct
    36481 tctctgccgt ttccaaatcg ccgctttgga cataccaacc gtaataacgg ttcaggcaca
    36541 gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc gcagaacatt acattgacac
    36601 aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc cgccagtggc gcaaaatatt
    36661 cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat actccacatc accacgcttg
    36721 ggtggttttt gtcacgcgca atcagctctt taatcgcctg taagtgcgcc tgctgggttt
    36781 ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa
    36841 tgcctaaaga gaggttaaag ccgacagctg cagtttcatc aatcaccacg attccatgtt
    36901 catctgccca gtcgagcatc tcttcagcgt aagggtaatg cgaggtacgg taggagttgg
    36961 ccccaatcca gtccattaat gcgtgatcgt gcaccatcag cacgttatcg aatcctttgc
    37021 cgcgcaaatc cgcatcttca tgacgaccaa agccagtaaa gtagaacggt ttgtggttga
    37081 tcaggaactg ttcgcccttc actgccactg accggatacc gacgcgcagc aggtagatat
    37141 cacactctgt ctggcttttg gctgtgacgc acagttcata gagataacct tcacccggtt
    37201 gccagaggtg cggattcact acttgcaaag tcccgctggt gccttgtcca gttgcaacca
    37261 cctgttgatc cgcatcacgc agttcaacgc tgacatcacc atttgccacc acctgccagt
    37321 caacagatgc gtggttacag tcttgcgcga catgcgtcac cacggtgata tcgtccaccc
    37381 aggtattcgg cgtggtgtag agcattacgc tgcgatggat cccggcgtag ttaaagaaat
    37441 catgaaagta agactgcttt ttcttgccgt tttcgtcggt aatcaccatt cccggcggga
    37501 tagtctgcca gttcagttcg ttgttcacac aaacagtgat acgtacactt ttcccggcaa
    37561 taacatacgg agtgacatcg gcttcaaatg gcgtatagcc gccctgatgc tccatcactt
    37621 cctgattatt tacccacact ttgccgtaat gagtgaccgc atcgaaacgc agtacaatac
    37681 gctggcccgc ccaacctttc ggtataaaga cttcgcgctg ataccagacg ttgcccacat
    37741 aattacgaat atctgcatcg gcgaactgat cgttaaaact gcccggcaca gcaattgccc
    37801 ggctttcttg taacgcgctt tcccaccaac gctgatcaat tccacagttt tcgcgatcca
    37861 gactgaatgc ccacaggccg tcgagttttt tgatttcacg ggttggggtt tctacaggac
    37921 gtaacataag ggactcctca ttaagataaa aatactggtc aacctttaat ctgattagtt
    37981 aaccaattct gtgttgcaca cagttatctg tgagaaaccc atggaagaga aatggaaata
    38041 aaaagaactg acaccgcgat caaaaaacca aaaactgcaa tacccacagc gtgatattga
    38101 tcgcattaat ctttaaaaca gcgttgacag aagccacaaa ataagcaatc gaacgttcgt
    38161 tcgaacatat tgggagcgat aaaccattat gatatattgc tacgatgaat atttaggtgg
    38221 gattctccga tccctgggag gatgattttt gcacagagaa attacgatat gaaacgcggg
    38281 cacagttgtc accagcccgc ggaaagagag agaggatcag gatgcggtta agataccgcc
    38341 aatcatcgcg cgtaaaccct gagcaaacga cgcttcacgc gccaggtctt ccgcttcgat
    38401 atcggccagt acaccgtagg tcagatccag cagtaaacgt gaagtcattg ccgtgttgat
    38461 gtctgggctt atctcgcctt tttgctgggc atcacgcatc cgctgggcaa caaattccgt
    38521 gatcgtcata tgcttatttt taagcatggc ggcgacctgt gggttacgca tcccttccgc
    38581 cataatttcg accaccagcg ctcgttgccc aaagacttca tgggtgagag agacaataga
    38641 ctcgaccata tagtcaacga aatgaatccc ttcaatcggt tcccggaaac gggccagcgc
    38701 cctctcctgg tcctgtaaga taatcgcctg aatcaaggct tctttggaga tgaaatggtg
    38761 atagagcgtc ccgggactaa tggcgcagga tttacagatg gctttcatcg aggcactgtg
    38821 aaatccattt tctgaaaaaa tctctctggc agcattgagg atccgggtcc gtgtcggttg
    38881 tgcttcagtc tgcatgttat ccatcattaa accattatag ttagcatgaa taactagttt
    38941 accgtgttgt caggtgagcg cctatcattg ctgttgaata gtgcgaaggc acactcaatt
    39001 cgtaagcttt ttatccagat attggttaac caatagccgt gatttggctg ccagttgttt
    39061 agcagagacg ttgatgagtt gcacacgcta tcccggtaaa caacacatac ggctgtttac
    39121 cggttcgtta gtgtattaat tgagctcctg taccccacca ccggagacgg tgagaatttg
    39181 tccgcttacc cagctggctg caggcgagca aaggaacagc gccgcgttag ctatatcttg
    39241 cggttggccc agacgtctga ttggtgtgtg ttgcaacatt ttctgttcaa tttctggtgt
    39301 aataacggat ttcagggcat cggttaatat tgcccccggc gcaatgccat tcacccgaat
    39361 atttttttca cccaggtcaa acgccatatt tctgaccaga tgactggccg cagctttaga
    39421 tgatgcatag gaggtcatgt ttatattttt attttctgcc gccatagaag taatggtcag
    39481 aataacgcca ccgccatttt tttccatttc tggcgcaaca agttgtgaca gatggaaaaa
    39541 agaaaataca ttcagttcat aagcgcggcg aaaatctgcc attggcatat caaacggttt
    39601 aggcccaccg ccaccggcgt tgttaaccag gatatcaact ttacccaact tactgacggc
    39661 aaagtctgcc agtgcagaga gttcctgttc ggaagtaata tcgcagcggc aggcaaatgc
    39721 ctgaccaccc agttgttgaa tttcatttac aacatgatta gctgcatcgg cgttaatatc
    39781 actgaccacc acagatgcgc cagctgtcgc gaatgtaatg gcgatttctt taccaatacc
    39841 tgcacccgca cctgtgatga tggcgcattt tccgtcgagt ctcaggttgt cagaattaaa
    39901 cacataaacc tcctgtgaac ttcagtaaaa ataaaaggca ccgcctgtgt gccacatttt
    39961 ttatcattaa ccttgaggaa aggacactac caggtatagc agataaagag aggattattt
    40021 ctccgcaatg agccgggcag gaataataag attgcgagaa tgcgtctctt catgggtgat
    40081 tttttgcatc atgcgatccg caagtgtggt accaagttcc cgcgctggcg tgctgaccca
    40141 ggtaacgggt atatcatcaa gtgtggttgg cgttgcatcg gtaaatgccg ccagcgaaac
    40201 ctgttgctca aagtaacgat cgactccgct ttccccgctt tgccttcctg ctttcagcaa
    40261 accaaaccat gcccccatcg caatagtttc gttatagcaa accacggcgc tgatggtcgg
    40321 gttatgacgt aaaagcgccg tgatagcttc cgcggcttgc ttctggctgg aagtgcactc
    40381 cagcacccaa tcgctgtgaa acggcaggcc aaattttagt agagtcgcac aatagccccc
    40441 cacccgttct gcccgggtta atgaggaact ttgtcctccc agccaggcga tacgctgatg
    40501 cccattgcga atgagatgct ccgtcaacaa ctgtgcagcc tgcatgttgt ccgggcgaac
    40561 cgtatcaacg tcatcaagat aactggcacg ggaagcgaaa atcaccggga tagctttttc
    40621 ttctgctatc cgtcgcaggt catcgctgct tcctgcagcc ccggcaatga ccacaccatc
    40681 gacaccctga ttcagtaata gtgaaaaccg ctgtgccagc tgctcgccgt ctttaccgcc
    40741 gtgaagcaaa aaaaccatcc gtccctgcgc ttccagagct tccgtcaatc cggccgtcaa
    40801 ttcggcgtaa aacggcgcag ataaatcacg gacgatcaaa ccaatgacgc cgctttgccc
    40861 gccgcgcagc gccgacgcct ggcgattgcg cacaaatccc agctcttcaa tggcggcgtt
    40921 aacgcgttct cctgtggcgg tagagattcg ccctttgcca ctaagcacca gcgaaacggt
    40981 acttaccgac acgcccgcag ccagcgcaac atcatgaatg gttatttttt tggcggtagc
    41041 catgcagatg acctactccc tgattatgtg acagataaaa cgttttatct tctcgcgcaa
    41101 tttattgaat ccagattgtt ctctacgagg agtcgtttta tgacggcgaa aacagcaccg
    41161 aaagtcacgc tgtgggagtt cttccagcag ttaggcaaaa ccttcatgtt acccgtggca
    41221 ttattgtcgt tctgcggcat tatgctcggc attggtagtt ctcttagcag ccatgatgtc
    41281 atcaccctga tcccggtcct gggcaacccc gtgttgcagg ctatctttac ctggatgagt
    41341 aagattggct cgtttgcttt tagtttcctg ccagtcatgt tctgtatcgc catcccgctg
    41401 ggtctggcac gcgaaaacaa aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta
    41461 atgaacctcg cggtaaactt ctggttgacc aataaaggca ttctgccaac cacggacgcc
    41521 gcggttctga aagccaataa catccagagc attcttggga tccagtcgat cgacaccggg
    41581 atcctcggtg cggtgatcgc cggtattatc gtctggatgc tgcatgagcg attccataat
    41641 atccgcctgc cggatgcgct ggcattcttc ggcggtacgc gcttcgtacc aatcatctcc
    41701 tcgctagtga tgggtcttgt cggcctggtg attccattag tctggccgat tttcgccatg
    41761 ggtattagcg gcttaggcca tatgatcaac agcgcgggag atttcggacc gatgctgttt
    41821 ggtaccggtg aacgtctgct gttgccgttt ggtctgcatc acattctggt ggcattaatt
    41881 cgctttaccg acgcaggcgg cacgcaggaa gtctgcggtc aaaccgtcag cggtgcactg
    41941 accatcttcc aggcgcaatt gagttgccca accactcacg gtttttctga aagcgccacg
    42001 cgtttccttt cgcaaggtaa aatgcctgcg tttctcggcg gtctgccagg tgcagcgtta
    42061 gctatgtatc actgtgcgcg cccggaaaat cgccataaaa ttaaaggtct gctgatttct
    42121 ggcctgatcg cctgtgtcgt tggcggcacg accgaaccgc tggaattcct gttcctgttc
    42181 gtagcgccag ttctgtatgt catccacgcg ctgttaaccg gcctcggctt cactgtcatg
    42241 tctgtgctcg gcgtcaccat cggtaatacc gacggcaata tcatcgactt tgtggtgttc
    42301 ggtattttgc atggtctgtc aaccaagtgg tacatggtgc cagtagtggc ggcaatctgg
    42361 tttgtcgttt actacatcat cttccgtttc gctatcaccc gcttcaatct caaaaccccg
    42421 gggcgcgata gtgaagttgc cagttcaatc gaaaaagccg ttgccggtgc gccgggtaaa
    42481 tcaggttaca acgttccggc aatcctcgaa gccttaggcg gtgccgacaa tattgtcagc
    42541 ctcgataact gcattacgcg tctgcgtttg tctgtaaaag atatgtcgct tgttaatgtg
    42601 caggcactga aggacaatcg ggcaattggc gtagtacaac ttaatcaaca taacctgcag
    42661 gttgttatcg ggccacaagt tcagtcagta aaagatgaaa tggccggtct gatgcatact
    42721 gtccaggcat aaggataaga tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca
    42781 tggtgtacgc agtgggatta tgtcgctgac cgtttcggca ctgctgacct gttaccgttc
    42841 acgatttcag acatggattt tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc
    42901 ctgatacacg gcgtgtttgg ctacagccgc tggaaaaacg atgagtttct cgcggctatt
    42961 gcccactggt tttccaccca gcattacacc gccatcgatc ctcagtcggt ggtgtatggc
    43021 ccttctgtta tctatatggt ttcagaactg attcgtcagt ggtctgaaac aggtgaaggc
    43081 gtggtgatcc acacacccgc ctatgacgca ttttacaagg ccattgaagg taaccagcgc
    43141 acagtaatgc ccgttgcttt agagaagcag cctgacggct ggttttgcga tatgggcaag
    43201 ctggaagccg tgctggcgaa accagaatgt aaaattatgc tcctgtgtag cccacagaat
    43261 cctaccggga aagtgtggac gtgcgatgag ctggagatca tggctgacct gtgcgagcgt
    43321 catggtgtgc gggttatttc cgatgaaatc catatggata tggtttgggg cgagcagccg
    43381 catattccct ggagtaatgt ggcgcgcgga gactgggcgt tgctaacgtc gggatcaaaa
    43441 agtttcaata ttcccgccct gaccggtgct tacgggatta tagaaaatag cagtagccgc
    43501 gatgcctatt tatcggcact gaaaggacgt gatgggcttt cttccccttc ggtactggcg
    43561 ttaactgctc atatcgccgc ctatcagcaa ggcgcaccat ggctggatgc cttacgcgtc
    43621 tatctgaaag ataacctgac gtatatcgca gataaaatga acgctgcgtt tcctgaactc
    43681 aactggcaga tcccgcaatc cacttatctg gcctggcttg atctacgtcc gttgaatatt
    43741 gacgacaacg cgttgcaaaa agcgcttatc gagcaagaaa agatcgcgat catgccggga
    43801 tatacctacg gtgaagaagg tcgtggtttt gtccgactca atgccggctg cccacgttcg
    43861 aaactggaaa aaggtgtggc tggattaatt aacgccatcc gcgctgttgg ttaaccccaa
    43921 ttgcgcaacg taaaaaatcg ttgcgcaatc gtggattttt accctgcttt gtttttataa
    43981 tggtgcgcac ttttatatcc agaaaaagag tgcgaccatg attgatacca ccctgccatt
    44041 aactgatatc catcgccacc ttgatggcaa cattcgtccc cagaccattc ttgaacttgg
    44101 ccgccagtat aatatctcgc ttcctgcaca atccctggaa acactgattc cccacgttca
    44161 ggtcattgcc aacgaacccg atctggtgag ctttctgact aaacttgact ggggcgttaa
    44221 agttctcgcc tctcttgatg cctgccgccg cgtggcattt gaaaacattg aagatgcagc
    44281 ccgtaacggc ctgcactatg tcgagctgcg tttttcacca ggctacatgg caatggcaca
    44341 tcagctgcct gtagcgggtg ttgtcgaagc ggtgatcgat ggcgtacgtg aaggttgccg
    44401 cacctttggt gtgcaggcga agcttatcgg tattatgagc cggaccttcg gcgaagccgc
    44461 ctgtcagcaa gagctggagg cctttttagc ccaccgtgac cagattaccg cacttgattt
    44521 agccggtgat gaacttggtt tcccgggaag tctgttcctt tctcatttca accgcgcgcg
    44581 tgatgcgggc tggcatatta ccgtccatgc aggcgaagct gccggaccgg aaagcatctg
    44641 gcaggcgatt cgtgaactgg gggcggagcg tattggacat ggcgtaaaag ccattgaaga
    44701 tcgggcgctg atggattttc tcgccgagca acaaattggt attgaatcct gtctgacctc
    44761 caatattcag accagcaccg tggcggatct ggctgcacat ccgctgaaaa cgttccttga
    44821 gcatggcatt cgtgccagca ttaacactga cgatccaggc gtgcagggag tggatatcat
    44881 tcacgaatat accgttgccg cgccagctgc tgggttatcc cgcgagcaaa tccgccaggc
    44941 acagattaat ggtctggaaa tggctttcct cagcgcagag gaaaaacgcg cactgcgaga
    45001 aaaagtcgcc gcgaagtaac aaaaatggat ggtgctaatt gcaccatccc cttttcacgc
    45061 aaggcacaaa gtcgcgcgat gtttggcgga ttcgatgccc agctcaatca actccattac
    45121 ctggattgcc tgacttgccg gaaccggatt ttcaccatcg ccatttaacg catcacgaat
    45181 agccgcatag taagccggat aattccccgg cactgtcaac agcgtttctt cgacacgttc
    45241 ctcaccttcc acgcgggtaa gtacgccatc gcgcatatcg tagccccagt cttcctgcgg
    45301 cagacgctcg ccatttttca gacgttcttc ctgtggatcg aggccatatt tcacataact
    45361 gcctcgggat ccatgcacga tataccgtgc tgactcggct gctgccagca tggtgccgtg
    45421 taaaatcact cgccgctgcg gataggacaa gatagcgtgg aaataatcgg tcgactgcgc
    45481 tccgggccgt aactgtgcca aatctaccgt catgctaacc ggtaaaccaa ataacgtaat
    45541 ggcctgatca agaaggtgcg gtgctaaatc gtaccagata ccgctgcctg ggccgccctg
    45601 ttcacgccaa cgatcgcgca cctgcgggcg gaagcggtca aaatgggact caacgtaagc
    45661 aatttcaccc agcacgcctt ccgcgagcaa acctttaagc gtcaggaaat cgctatccca
    45721 gcgacggtta tggaatacag acagcactcg ccccaggctt tttgccagcg catccagctc
    45781 tcgcgcttgt gacagtgtca cggtaaaggg tttatcaacg accacatgtt tacccgcctc
    45841 aagcgccgct ttggctaacg gaaaatgggt atcgttgggt gtaggaatga caatcaggtc
    45901 tatgttggga tcgttaaaca gatgcttcgg ctcagagaca accgtaaccg ttggccagtc
    45961 agcttttact tttgtttcat cgctgctgga gattaccgcc agctccagcc cgggcgtgcc
    46021 cgcaatcagg ggcgcatgga aggttttgct cgcataacca tacccaatca acccaacacg
    46081 gatgttgtcg ctcatgtcat tgcctctcat tgaagtatga tggctatttg acactatcct
    46141 ttaccctcgc tcaacagttt aataacctgc cagcaataag ggatgttgtt taacttaagt
    46201 caaaaaaata gcgatttttc caacgacaaa agctaaatat tgcaaaaacc tcagtaaaaa
    46261 tcttgctgga gctattattg ctaagtaaca tttaccccct gaagttaatg gatcaatcaa
    46321 cagagatgtg ggctgtaatg aatcgtctta ttgaattaac aggttggatc gttcttgtcg
    46381 tttcagtcat tcttcttggc gtggcgagtc acattgacaa ctatcagcca cctgaacaga
    46441 gtactacagt acaacacaag taagctctgc acttgttgag cgacatgctg cccgtccggg
    46501 tgcatgtttt cacttgtcgg atattaaacc aggaatttat tatcttgttc gatgttgttg
    46561 atgattgtca gggatagtaa agcgcgttac tctttccaga gcagtttttc atccctgaat
    46621 atttgccatc agcacattct tattattgta ataacacaag cgttcacttt tggtgaaatt
    46681 ttatcatcac taataatacc tacccctttg gtattgatta ttttatggac cttttatgac
    46741 tgttcaggac tacttattaa aatttcgcaa aatcagttca ctcgaaagtc tggaaaaact
    46801 ctacgatcat cttaattaca ccctgacgga cgatcaggaa ctgatcaata tgtatcgtgc
    46861 tgccgatcac cgtcgcgcag agctggtttc tggcgggcgt ttgtttgacc tcggccaggt
    46921 accgaagtcc gtctggcact atgtccagta aagaaagtag cgatcggtta taaaagcctt
    46981 ataataacgc ccctaaaatt agatttgtct gtttgcgtcg ctattctgga gaacctatga
    47041 ccaccacaac gccacaacgt attggaggct ggttgcttgg cccgttggcc tggcttttag
    47101 tcgctctgtt gagtacgacg ctggcgttgt tgctgtatac cgctgcgtta tcttctccgc
    47161 aaacatttca aacgctcggc ggacaagcac tgacgacaca aatcttatgg ggcgtttctt
    47221 ttattaccgc tatcgccatg tggtattaca cactgtggtt gaccatcgct ttctttaaac
    47281 gtcggcgttg tgttcctaaa cactatatta tctggctgct tatttccgta ttgctggcag
    47341 taaaagcgtt cgctttttca cccgtagaag atggcatagc cgttcgccag ttgctgttta
    47401 ctttgttggc aaccgcattg atcgtcccct atttcaaacg ctcgtcgcgg gtgaaagcga
    47461 cgtttgtgaa tccgtaataa ccttacagtt aacctgttgt cgcctgctct ggattaacgg
    47521 ataataggcg gcttttttat ttcaggccga aaaatgactg actacctgtt actgtttgtc
    47581 ggaactgtac tggtcaataa ctttgtactg gtcaagtttc tcggtctctg tccgtttatg
    47641 ggggtttcca aaaagctgga aaccgcgatg ggcatggggc tggcaacaac gtttgtgatg
    47701 acgctggcgt ctatttgcgc ctggcttatc gatacgtgga ttttgatccc acttaatctg
    47761 atatacctgc gcaccctggc gtttattctg gtgattgctg tggttgtgca gttcaccgaa
    47821 atggtggtgc gcaaaaccag cccggtactt taccgtctgc tggggatttt tttgccgctt
    47881 atcactacca actgtgccgt gctcggcgtg gcgttgctga atatcaatct cgggcacaat
    47941 ttcttgcagt cagcgctgta cggtttttcc gccgctgtcg gtttctcgct ggtgatggtg
    48001 ctcttcgccg ccatccgcga acgccttgct gtggctgatg tcccggcacc ttttcgcggt
    48061 aatgccattg cgttaattac cgcaggtctt atgtctctgg cctttatggg ctttagtggt
    48121 ttggtgaagt tgtaatgaat gctatctgga ttgccgttgc cgccgtgagc ctgctgggcc
    48181 tggcgtttgg cgccattctg ggttatgcct cccgccgttt tgcggtggaa gacgatccgg
    48241 tcgttgagaa aattgacgaa atcttaccgc agagccagtg tggtcagtgc ggttatcccg
    48301 gctgtcgccc ctacgcggaa gccatcagct gtaacggtga aaaaatcaac cgttgcgccc
    48361 caggtggcga agctgtgatg ctaaaaattg ccgagttgct taatgtcgag ccgcagccgc
    48421 tggatggcga agcgcaagag ctaacgccag cgcggatggt ggcggtgatt gatgaaaata
    48481 actgtattgg ctgcactaaa tgtattcagg cgtgtccggt agacgccatc gttggcgcta
    48541 cccgcgccat gcatacggta atgagtgatc tctgtacggg ctgcaattta tgtgttgatc
    48601 cgtgcccgac gcactgcatc tcgttgcaac cggtcgcaga aactcctgac tcctggaaat
    48661 gggatctgaa caccattccc gtgcgtatca ttcccgtgga acaccatgct taagttattc
    48721 tctgcattca gaaaaaataa aatctgggat ttcaacggcg gcatccatcc accggagatg
    48781 aaaacccagt ccaacggtac gcccctgcgc caggtaccac tggcgcagcg ttttgtcatt
    48841 ccactgaaac aacatattgg cgctgaaggt gagttgtgcg ttagcgtcgg cgataaagta
    48901 ttgcgcggcc agccgcttac ccgtggtcgc ggcaaaatgc tgccggttca cgcgcccacc
    48961 tcgggtaccg ttacggctat tgcgccccac tctacggctc atccttcagc tttagctgaa
    49021 ttaagtgtga ttattgatgc cgatggcgaa gactgctgga tcccgcgcga cggctgggcc
    49081 gattatcgct ctcgcagtcg cgaagagtta atcgagcgca ttcatcagtt tggtgttgcc
    49141 gggctgggtg gtgcgggatt tccgacaggc gttaaattgc agggcggcgg agataagatt
    49201 gaaacgttga ttatcaacgc tgctgagtgc gagccgtaca ttaccgccga tgaccgtttg
    49261 atgcaggatt gtgcggctca ggtcgtagag ggtatccgca tccttgcgca tattctgcag
    49321 ccacgcgaaa ttcttatcgg tattgaagat aacaaaccgc aggcgatttc catgctgcgc
    49381 gcggtgctgg cagactctca cgatatttct ctgcgggtga ttcccaccaa atatccttct
    49441 ggcggtgcta aacaattaac ctacattctg accgggaagc aggttccaca tggcgggcgt
    49501 tcatccgata tcggcgtatt aatgcaaaac gtcggcaccg cttatgcagt gaaacgtgcc
    49561 gttattgatg gcgagccgat taccgagcgt gtcgtgaccc tgactggcga agcaatcgct
    49621 cgcccgggca acgtctgggc acggctgggt acgccagtgc gtcatttatt gaatgatgcc
    49681 ggattctgcc cctctgccga tcaaatggtg attatgggcg gcccgctaat gggctttacc
    49741 ttgccgtggc tggatgtccc ggtcgtaaag attaccaact gtcttttggc tccctctgcc
    49801 aatgaacttg gcgaaccaca ggaagaacaa agttgcatcc gttgtagcgc ctgtgctgac
    49861 gcctgccctg cggatctttt gccgcaacag ctgtactggt tcagcaaagg tcagcaacac
    49921 gataaagcca ccacgcataa cattgccgac tgcattgaat gtggagcttg cgcgtgggtt
    49981 tgcccgagca atatccccct ggtgcaatat ttccgtcagg aaaaagctga aattgcggct
    50041 attcgtcagg aagaaaagcg cgccgcagaa gccaaagcgc gtttcgaagc gcgccaggct
    50101 cgtctggagc gcgaaaaagc ggctcgcctt gaacgacata agagcgcagc cgttcaacct
    50161 gcagccaaag ataaagatgc gattgctgcc gctctggcgc gggtgaaaga gaaacaggcc
    50221 caggctacac agcctattgt gattaaagcg ggcgaacgcc cggataacag tgcaattatt
    50281 gcagcacggg aagcccgtaa agcgcaagcc agagcgaaac aggcagaact gcagcaaact
    50341 aacgacgctg caaccgttgc tgatccacgt aaaactgcag ttgaagcggc tattgcccgc
    50401 gccaaagcac g
//
LOCUS       DADTFG010000011        49874 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.011, whole genome shotgun sequence.
ACCESSION   DADTFG010000011 DADTFG010000000
VERSION     DADTFG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.011"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            44..1093
                     /locus_tag="J1138_000691"
     CDS             44..1093
                     /locus_tag="J1138_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBA8452533.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQQSGTNSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1167..2024
                     /gene="nfo"
                     /locus_tag="J1138_000692"
     CDS             1167..2024
                     /gene="nfo"
                     /locus_tag="J1138_000692"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBA8452534.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLHGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2028..3116
                     /locus_tag="J1138_000693"
     CDS             2028..3116
                     /locus_tag="J1138_000693"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA8452535.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3384..4325)
                     /gene="rihB"
                     /locus_tag="J1138_000694"
     CDS             complement(3384..4325)
                     /gene="rihB"
                     /locus_tag="J1138_000694"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBA8452536.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            4455..5153
                     /gene="yeiL"
                     /locus_tag="J1138_000695"
     CDS             4455..5153
                     /gene="yeiL"
                     /locus_tag="J1138_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBA8452537.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LKMYPENKFSGMMQ"
     gene            complement(5220..6470)
                     /locus_tag="J1138_000696"
     CDS             complement(5220..6470)
                     /locus_tag="J1138_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBA8452538.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPSGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6564..7502)
                     /locus_tag="J1138_000697"
     CDS             complement(6564..7502)
                     /locus_tag="J1138_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBA8452539.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(7490..8431)
                     /locus_tag="J1138_000698"
     CDS             complement(7490..8431)
                     /locus_tag="J1138_000698"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBA8452540.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(8887..10578)
                     /gene="fruA"
                     /locus_tag="J1138_000699"
     CDS             complement(8887..10578)
                     /gene="fruA"
                     /locus_tag="J1138_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBA8452541.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(10595..11533)
                     /gene="fruK"
                     /locus_tag="J1138_000700"
     CDS             complement(10595..11533)
                     /gene="fruK"
                     /locus_tag="J1138_000700"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBA8452542.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(11533..12663)
                     /locus_tag="J1138_000701"
     CDS             complement(11533..12663)
                     /locus_tag="J1138_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBA8452543.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            13030..14211
                     /gene="setB"
                     /locus_tag="J1138_000702"
     CDS             13030..14211
                     /gene="setB"
                     /locus_tag="J1138_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBA8452544.1"
                     /translation="MHNSPAVTSAKSFDLTSMAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(14208..14462)
                     /locus_tag="J1138_000703"
     CDS             complement(14208..14462)
                     /locus_tag="J1138_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBA8452545.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            14617..15189
                     /gene="yeiP"
                     /locus_tag="J1138_000704"
     CDS             14617..15189
                     /gene="yeiP"
                     /locus_tag="J1138_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBA8452546.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            15412..16878
                     /locus_tag="J1138_000705"
     CDS             15412..16878
                     /locus_tag="J1138_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA8452547.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNLRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEIA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTRQIAMDGSQKLPQRMLAGIRIHLRRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            16996..17982
                     /locus_tag="J1138_000706"
     CDS             16996..17982
                     /locus_tag="J1138_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBA8452548.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            18021..18734
                     /gene="lpxT"
                     /locus_tag="J1138_000707"
     CDS             18021..18734
                     /gene="lpxT"
                     /locus_tag="J1138_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBA8452549.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            19146..19712
                     /gene="mepS"
                     /locus_tag="J1138_000708"
     CDS             19146..19712
                     /gene="mepS"
                     /locus_tag="J1138_000708"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBA8452550.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            19893..21449
                     /locus_tag="J1138_000709"
     CDS             19893..21449
                     /locus_tag="J1138_000709"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBA8452551.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            21531..23345
                     /locus_tag="J1138_000710"
     CDS             21531..23345
                     /locus_tag="J1138_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA8452552.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYAEDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            23346..24440
                     /locus_tag="J1138_000711"
     CDS             23346..24440
                     /locus_tag="J1138_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBA8452553.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            24440..25465
                     /locus_tag="J1138_000712"
     CDS             24440..25465
                     /locus_tag="J1138_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8452554.1"
                     /translation="MPRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDS
                     FDPNKAV"
     gene            25467..27056
                     /gene="yejF"
                     /locus_tag="J1138_000713"
     CDS             25467..27056
                     /gene="yejF"
                     /locus_tag="J1138_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBA8452555.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIAAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(27060..27404)
                     /locus_tag="J1138_000714"
     CDS             complement(27060..27404)
                     /locus_tag="J1138_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452556.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(27737..28927)
                     /gene="bcr"
                     /locus_tag="J1138_000715"
     CDS             complement(27737..28927)
                     /gene="bcr"
                     /locus_tag="J1138_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBA8452557.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTVSSLAGTFRFGIGAIVGALLSLATFNSAWSMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(28955..29650)
                     /gene="rsuA"
                     /locus_tag="J1138_000716"
     CDS             complement(28955..29650)
                     /gene="rsuA"
                     /locus_tag="J1138_000716"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBA8452558.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            29800..31560
                     /locus_tag="J1138_000717"
     CDS             29800..31560
                     /locus_tag="J1138_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA8452559.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            31685..31969
                     /gene="rplY"
                     /locus_tag="J1138_000718"
     CDS             31685..31969
                     /gene="rplY"
                     /locus_tag="J1138_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBA8452560.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(32108..33115)
                     /gene="yejK"
                     /locus_tag="J1138_000719"
     CDS             complement(32108..33115)
                     /gene="yejK"
                     /locus_tag="J1138_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBA8452561.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            33297..33524
                     /locus_tag="J1138_000720"
     CDS             33297..33524
                     /locus_tag="J1138_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBA8452562.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSITEDKAH"
     gene            33544..35304
                     /gene="pbgA"
                     /locus_tag="J1138_000721"
     CDS             33544..35304
                     /gene="pbgA"
                     /locus_tag="J1138_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBA8452563.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            35379..35455
                     /locus_tag="J1138_000722"
     tRNA            35379..35455
                     /locus_tag="J1138_000722"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35413..35415,aa:Pro,seq:ggg)
     gene            complement(35566..36509)
                     /locus_tag="J1138_000723"
                     /pseudo
     CDS             complement(35566..36509)
                     /locus_tag="J1138_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247409.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            36829..37476
                     /gene="narP"
                     /locus_tag="J1138_000724"
     CDS             36829..37476
                     /gene="narP"
                     /locus_tag="J1138_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBA8452564.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(37511..38563)
                     /locus_tag="J1138_000725"
     CDS             complement(37511..38563)
                     /locus_tag="J1138_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HBA8452565.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSLQESK"
     gene            complement(38560..39117)
                     /gene="dsbE"
                     /locus_tag="J1138_000726"
     CDS             complement(38560..39117)
                     /gene="dsbE"
                     /locus_tag="J1138_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBA8452566.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(39114..41057)
                     /locus_tag="J1138_000727"
     CDS             complement(39114..41057)
                     /locus_tag="J1138_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBA8452567.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(41054..41533)
                     /gene="ccmE"
                     /locus_tag="J1138_000728"
     CDS             complement(41054..41533)
                     /gene="ccmE"
                     /locus_tag="J1138_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBA8452568.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(41530..41739)
                     /gene="ccmD"
                     /locus_tag="J1138_000729"
     CDS             complement(41530..41739)
                     /gene="ccmD"
                     /locus_tag="J1138_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBA8452569.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(41736..42473)
                     /gene="ccmC"
                     /locus_tag="J1138_000730"
     CDS             complement(41736..42473)
                     /gene="ccmC"
                     /locus_tag="J1138_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBA8452570.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(42515..43177)
                     /gene="ccmB"
                     /locus_tag="J1138_000731"
     CDS             complement(42515..43177)
                     /gene="ccmB"
                     /locus_tag="J1138_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBA8452571.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAAVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(43174..43791)
                     /gene="ccmA"
                     /locus_tag="J1138_000732"
     CDS             complement(43174..43791)
                     /gene="ccmA"
                     /locus_tag="J1138_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBA8452572.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(43810..44412)
                     /gene="napC"
                     /locus_tag="J1138_000733"
     CDS             complement(43810..44412)
                     /gene="napC"
                     /locus_tag="J1138_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBA8452573.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKILGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(44422..44871)
                     /gene="napB"
                     /locus_tag="J1138_000734"
     CDS             complement(44422..44871)
                     /gene="napB"
                     /locus_tag="J1138_000734"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBA8452574.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADIAPIVGNTFTPSKGYGK"
     gene            complement(44868..45731)
                     /gene="napH"
                     /locus_tag="J1138_000735"
     CDS             complement(44868..45731)
                     /gene="napH"
                     /locus_tag="J1138_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBA8452575.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(45718..46413)
                     /gene="napG"
                     /locus_tag="J1138_000736"
     CDS             complement(45718..46413)
                     /gene="napG"
                     /locus_tag="J1138_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBA8452576.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(46420..48906)
                     /gene="napA"
                     /locus_tag="J1138_000737"
     CDS             complement(46420..48906)
                     /gene="napA"
                     /locus_tag="J1138_000737"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBA8452577.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYDVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(48903..49166)
                     /gene="napD"
                     /locus_tag="J1138_000738"
     CDS             complement(48903..49166)
                     /gene="napD"
                     /locus_tag="J1138_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBA8452578.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(49156..49650)
                     /gene="napF"
                     /locus_tag="J1138_000739"
     CDS             complement(49156..49650)
                     /gene="napF"
                     /locus_tag="J1138_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBA8452579.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT        12197 a        13391 c        12277 g        12009 t
ORIGIN      
        1 aaccagttag tcgtaacctt atgcccggtt aaaggagagg gttatgacga atatcacctt
       61 acagaaacaa catcgtacac tgtggcattt tattccgggg ttagccctga gtgcagttat
      121 caccggggtc gccctgtggg gtggttccat tcctgcggtt gcgggtgccg ggtttagtgc
      181 cctcaccctc gcaatcttgt tggggatggt tttaggcaac accatttacc cgcacatctg
      241 gaaaagctgt gacggtggtg tgctgtttgc caagcaatat ttattacgtc tgggtatcat
      301 tctttatggc ttccgtctga cgttctcgca aattgccgat gtcggtatca gtgggatcat
      361 cattgacgtg ttgacgctgt ccagtacctt cctgctggct tgcttcctgg ggcagaaagt
      421 gtttggtctg gataagcaca ccagctggtt gatcggtgcc ggtagcagta tctgtggtgc
      481 tgccgcggta ctggcgactg agccggtagt gaaagcagaa gccagtaaag taaccgtggc
      541 tgttgcaacc gttgttatct tcgggaccgt cgcgattttc ctctacccgg cgatatatcc
      601 gctgatgtct caatggttta gtccggaaac tttcggtatc tacattggtt ctactgtgca
      661 cgaagtggcg caggtggtgg cggcaggtca tgccatcagc ccggatgcag aaaacgcagc
      721 agttatttcc aaaatgctgc gcgtgatgat gctggctccg ttcctcatcc tgctggcggc
      781 gcgtgttaaa cagcagtctg ggacgaacag cggcgagaaa agcaaaatca ctattccgtg
      841 gtttgccatc ttgttcatcg tcgttgccat ctttaactcg ttccacctgt taccgcagag
      901 cgtggtgaac atgctggtaa cgctggatac cttcctgctg gcaatggcga tggcggcgct
      961 ggggctgacc actcacgtca gcgcgctgaa aaaagccggg gcgaaacctc tgctgatggc
     1021 actggttctg tttgcctggc tgattgttgg tggtggtgcg attaactatg tgattcaaag
     1081 cgtcatcgca taaaccacta catcttgctc ctgttaaccc gctatcatta ccgttttcct
     1141 ccagcgggtt taacaggagt cctcgcatga aatacattgg agcgcacgtt agtgctgctg
     1201 gcggtctggc aaatgccgca attcgcgccg ccgaaatcga cgcaaccgcg tttgccttgt
     1261 tcaccaaaaa ccaacgtcag tggcgtgccg caccgctcac gacgcaaacc atcgatgaat
     1321 tcaaagccgc ctgtgaaaaa tatcactaca catcggcgca aattcttccc cacgacagtt
     1381 atctgattaa cctcggacat ccggttgctg aagctctgga aaaatcgcgc gatgccttta
     1441 tagatgaaat gcagcgttgc gaacagctgg ggctttcttt gctcaacttc caccctggca
     1501 gccatctgat gcagatttca gaagaggatt gccttgcgcg tattgccgaa tccatcaaca
     1561 ttgcgctgga taaaactcaa ggtgtcaccg cggtgattga aaacaccgcc ggtcagggca
     1621 gtaacttagg gtttaaattc gaacatctcg cggcgattat cgacggcgtg gaagataaat
     1681 cccgcgtcgg cgtctgcatt gatacctgcc atgctttcgc tgccgggtat gatttgcgta
     1741 ctcctgccga atgcgagaaa acgttcgctg attttgcccg tattgtaggc tttaagtatc
     1801 tgcacgggat gcacctcaac gatgcgaaaa gcacctttgg cagccgcgtt gaccgccatc
     1861 atagcctcgg cgaaggcaat atcggtcatg atgcgttccg ctggatcatg caagacgacc
     1921 gtttcgacgg cattccgctg atccttgaaa ccatcaaccc ggatatctgg gcagaagaga
     1981 tcgcctggct gaaagcgcaa caaactgaaa aagcggtggc ctgatcgatg aacgacaggg
     2041 aaaagcagat tcttaaaatt ttacggcgta acccgctgat tcaacagaat gaaatcgccg
     2101 atattctgca aatcagccgc tcacgtgttg ccgctcatat tatggatttg atgcgcaagg
     2161 gtttgattaa aggcaaagga tatatcctta ccgagcagga ttactgtgtg gttgtgggtg
     2221 caatcaatat ggatattcgc ggaatggcgg atatccgtta cccgcaagca gcctctcatc
     2281 caggttctgt tcactgttcc gcaggcggcg tcggacgtaa tatcgcgcat aacctggctc
     2341 tgcttggtcg ggatgtgcat ttaatatcag ctattggcaa cgatttttac ggcgagacat
     2401 tactggaaga aacgcgccgc gcgggcgtga acgtttcaaa ctgcattcgt ctgcatggac
     2461 acagcactgc aacttatctc gctatcgcta ataagcagga agaaactatc ctggcaatca
     2521 atgacacaca tattctgcaa cagctaacgc ctcaactgct caatacttca cgggacttga
     2581 ttcgccatgc tggtgtggtg ctggctgatt gtaatctcac acctgaagcc ctggagtggg
     2641 tctttaccat tgctgatgaa attccgatgt ttgtcgatac cgtttccgaa tttaaagcga
     2701 acaaagtaaa aaactggtac agccggatcc acactctgaa accaacacaa aatgaactgg
     2761 aaattttgtg ggggcaaccg attaaagacg acaacgatcg catcagagct gtaaattcgc
     2821 tgcatcaaca aggtgttaaa cgcatttttg tttatctgaa agatgaatct gtattttgca
     2881 gcgataagga tggagaacaa tttttgctga ccgcgccagc gcatacgaca gtagacagtt
     2941 ttggtgctga cgatggtttt atggcgggcc tggtatatag ctttcttgaa ggatacagtt
     3001 tccgcgacag cgcccgtttt gcgatggcct gcgcggcaat ttcgcgcgcc agcggcagct
     3061 taaacaaccc taccctgtct gccgataacg cgctttcatt agtgccaatg gtgtaacaat
     3121 gttgccggat tcgacgcttg accgcatctt atccggctca taaattgcgc actacgctaa
     3181 accaataaag aatcctgcaa tagtagcact catcaggtta ctgacagcaa aaacgcaata
     3241 gccagtaata ccaccattcc cagaacactt ctcatgacat ccatagttat tatctcttca
     3301 tgtcattttg ggcgacggct ctctgcccat cataaatagc cgggcctgca tcttgcgata
     3361 ctgacccggc ttgttatcaa cacttaatgg gtttttatgt agccgcgtac gcactcttcg
     3421 actaatcccc agaaccagtc ggtatcaata gtgatgccga cttttgtatt ggcgggcttg
     3481 ccaagaacgc ccagctcgtc gcagacggta cgcccgtaac aagggccact gttgacgtcc
     3541 acttcgacgt acatctcctg ggttttaatg ccatcggggt taatcagata accgatacag
     3601 gtggcgtcat gtaccgggcc gccagccagg ccgtagtttt cgaactgcgt tttgagagtg
     3661 aagttcatga tgtcgctgaa cagctctccg gcagggccgc cagccctttc catccgagca
     3721 atcacatccg gggtgcaaac ggtctggttg gtaagatcga ggcccatcat cactaatgga
     3781 acgccggagg tgaacaccac gcgtgcggcc tccgggtcag caaagatgtt gaactcagca
     3841 gatggggtga agttgccagt accataagca ccgcccatca gaacaatttc acggattttc
     3901 ggcaggatcg cgggttgcat acgcattgcc accgcgatgt ttgacaacgg accaaccggc
     3961 accagagtga tatcgccatc gcttgccata agggtatcga tgatatattt caccgcatga
     4021 gtgttttctg cctggcgggt cagcggctcg aataccgggc catccagtcc ggtttcaccg
     4081 tgaatattat cggcaacaat ttgttgacgc ataatgggct gcggcatccc cgcataaacc
     4141 ggaacattaa tctccagttt ctggcaaaca ttcaggccat taattaatgt tttatcgagg
     4201 gtttgattac ccgctacaat ggtgatacct aataaatcaa tcgccggatg tttcgccgcc
     4261 atcattatag caatagcatc atcatgaccc ggatcacaat ccagaataat ttttctcttt
     4321 tccattgttt atttcctctg tttccagttg cgttattgtt tctacagcaa agaaaattcg
     4381 acctggccga tgattgaatc ttaacaacag cgtacgtatg ctaaatatga gaaatctcat
     4441 agcggataaa catcgtgaaa gaaatccaca ataatgatct taagcagcaa ttgatgagtg
     4501 aatctgcgtt taaggattgc ttttcaacgg atgtttcagc cgatacgcgg ctgtttcatt
     4561 ttttagcgcg tgactacatt gtgcaggaag ggcaacagcc gtcctggctg ttttacctga
     4621 cgcgaggccg cgccaggctt tacgtcacgc ttgctaatgg tcgcgtgtcg ctaatcgatt
     4681 tctttgccgc gccctgtttt attggcgaga ttgagttaat cgacaaagac catgaaccgc
     4741 gtgcagtgca ggctattgaa gagtgttggt gccttgcgct ccctatgaag cattaccgcc
     4801 cgttgttatt aaacgacacg ctatttttac gaaaactctg cgtcacctta agtcataaaa
     4861 attatcgtaa tattgtttct ttaactcaga atcaatcatt tccgttagtg aatcgcctgg
     4921 cagcttttat attactctcg caggaaggtg atctttatca cgaaaagcat acgcaagcgg
     4981 cagagtattt aggcgtttct tatcgacatc ttttatatgt tcttgcgcaa ttcattaatg
     5041 acggtttatt aattaaaagc aagaaagggt atctcattaa aaacagaaag cagttgtcag
     5101 gactggcgct gaagatgtac ccggagaata aattctccgg gatgatgcag taaaaattat
     5161 tggcaatagt gcgattgccg gatgcaacgc ttaataaggc gtacatccgg caatgaaact
     5221 caagctaaac caataaagaa cccggcaatg gtcgcgctca tcaggttgga aagcgtcgcc
     5281 gccgccagcg cccgtaaacc aagctgggcg atttccggcg cgcgttttgg cgaaatagcc
     5341 gaaaacgctc caacgacaac accgatagaa ccaaagtttg caaacccaca gagggcgaac
     5401 gagataatgg caatggtctt gacatccagc gtgccgctcg tttgcagata gggcgagaaa
     5461 ttgagataag caacaaactc attaatcgcc agtttctgcc caatcaaact tccggcaaga
     5521 tttgcatcac tccagtccac ccccataatc catgccaacg gggccagcac atagccgaaa
     5581 atgctttcca gagagacatt ggcaaaacca aaccagccgc caactccacc aataatgccg
     5641 ttgatcagcg caatgatcgc cacaaatgcc attaccaccg tcgccacacc ggcggcgatt
     5701 ttcagcccag tcattgcacc gttcgccgca gcttcaataa tgctttttgg cggtgtttcg
     5761 ctaaacgaca gattttcaaa cgtaacttgc gaaggttccg tcgccggact aagaatacgt
     5821 gcaaacagga taccgctagg aatagccatc agcgaagcgg ctaacaaata gtcaatcggc
     5881 acgcccattc cggcatagcc aatcatcatc gaacccgcaa tagacgccat gccgctacaa
     5941 atagccgtaa atagctcgtt gcgattcatg cgatcaataa aaggtttcac gatcgccggg
     6001 atctcatttt gcccgaggaa aatagtggtg actgcgacaa aagattcgat tttgctgatg
     6061 ttgagggctt tctgaaaaat actgccaaga atacgaatca gcagccccat tacgccaatg
     6121 tagtacagca agctgataag cgcggtaacg aaaataatcg ccgggagtac gcgaaaggcg
     6181 aagataaagc ccgcaccgtc aaacagaaca tccattttgg gaccgaccag cgaaccaaaa
     6241 ataaaggcac ttcctgcgtc actgtaggac atcactttat ggacgcctaa tgccgcctgc
     6301 tctactgccc attttcccgg gggaaaatag agcatgatgc caccaatggc aatttgcagc
     6361 agcagcgcag ccccaaccgt gcgcaagctg atgctctttt tatttaccga caacagaaat
     6421 gctattgcca gtaacaccac catccccaca acacttctca ttatatccat aatgattttc
     6481 ccttcatgcc agtaaacccg gcgtcagcgc caggttttgg tatgtttgat gagtacgggc
     6541 gacggctttc tgcccaccag aaattaaccc acgagacgct gatattcttt ggcaatttcg
     6601 ctcgccagaa tggcgttatt aaagaccagt tgaatattgg atttcaggct atcaccgccg
     6661 gttagctccg caacacgcgc cagcaggaac ggtgtactct ctttcccaat aacaccctgt
     6721 tcttcggctt cagcaacggc ttgatcaatt gctgcattga tggtttcttc tggcatcgca
     6781 aactgttccg ggatcgggtt cgctaccacc aggccgccgt tcaaaccggt ttgccacttc
     6841 actgccatcg cgcgagcaat ttcgcgggca ctgtcgagac gaatgctgac atcaaatggg
     6901 ctggttcggc agaaaaacgc aggcagcgct tttgtctgat aaccaattaa cggtacaccg
     6961 aaggtttcta aatattctgt tgttaatcct aaatcgagaa tagattttgc ccccgcacaa
     7021 acaacagtga cattggtatt tgccagttcc tgtaaatcgg cagaaatatc gaaggtatgt
     7081 tccgctccgc gatgcacacc accaatacct ccggttgcaa aaactttaat cccagccagt
     7141 gctgcaataa tcatggttga tgcaacggta gtcgcgccat ttttccctgc agcaacgaca
     7201 aaaggtaaat cacgacgact aactttcgtc acgttatgtc cttcacggcc cagcaattcg
     7261 atttcttctt tacttaagcc cactttcatc acaccgccaa taatggcgat agtggctggt
     7321 actgcgccct gtttacgaat tgtttcttct acttcaattg cggtttgggc attttgtggg
     7381 aatggcattc cgtgagaaat tatggtcgat tccagcgcaa caaccggttt tttatttttt
     7441 aaagcatcct gtacttcagc ggatatttgt aataattcag gggacagagt taattcagac
     7501 attctgtgtt ctccactaac gatgtaacat tggcaattga taattcaggg ttgttggtat
     7561 attcacaggc aagtgccatt gacgaacatc cctgcgcaaa tcgaacagaa tcgataaacg
     7621 gcataccgtc tacccaacag gaggccagcc ccgccatcat ggcgtcgcct gccccagtaa
     7681 cattgatgac attcgtttta ataggggccg accacccact ttcaccgttg atatcactgt
     7741 agtaaacgcc atcccctccc atgctaagca ccaggcggtt caggccgtgt tgatgaaacc
     7801 aggcggcgac ctttgcgaca tcttcacgtc cggaaagtgc aattccgctc aacgtttcgg
     7861 cttcaagacg gttaggtttt aacgtatgga ttttgctcag atgatcgcgg attttgacgc
     7921 atttccatgc cgataccgga tcaataaata caggcgtttc cccgctattt tccagaaccc
     7981 agaccagcgc ctcttcacta aggttgcaat ccgcgacaat aacctttgca ccgcgaataa
     8041 actcctgatg ctgggcaaga aattctgcag aaatacagtc actaatactc atatcattta
     8101 tcgcgacaag catttctcct gtattatcga gcagtgataa atagctggat gtattctcac
     8161 ccggaacaat taagcattta tcgacataaa caccggattg attagtttgc gccagtaatg
     8221 attgcccata aaaatcacca cctactgcac ttaaaagcca ggcatttttc cccagtaacg
     8281 ccagattatg agcgatattc cttcccacgc cacctggggt aaatttaatt tttccgggat
     8341 tcgaatccgc ataattcaat ggatgatgtg aatacccagc cacatcaata ttcgccgagc
     8401 caataattac tatgtagtcc ttttcgcgca tagcacgccc cctggcaata aattgccaac
     8461 gaagaaattg ggtaaaatag tcattacagt caataagact attagagcac gtgttcagat
     8521 ttaaacacat gttcataata agcatgttta acgcaatgta aatcgatcag aaaaaacgaa
     8581 atcggccaga tatgaaggcg gtcacaaaat ttgtctattt atgcagcaac aaaagagaaa
     8641 gttgtgatgt gtaacacaca atatggcagg caagaaaaag ggcagagatg tttctgccct
     8701 cagattgttg acaatgcgcg ctttgttcat gctggatgtg gcgtaaacgc cttatccgac
     8761 ctacaaaagc gcaaaaattc aatatattgc agggttcatg taggtctgat aagcatagcg
     8821 catcaggcaa ttttgcgttt gtcatcatcg gggcagagat gtctcagccc cgatgatgaa
     8881 gagaaattat gcgttttttt ctactatttg tgtttccggg cgtttgagga aggcataggc
     8941 caaacccgcc accagcgtac cagcaataat tgctaccagg taacccaata ccggcgtaat
     9001 agcgccaggg atcagcagaa caaacagacc accgtgtggt gccatcagtt tcgcaccaat
     9061 cgccattgag attgcgccag tcagcgcccc accaacgata cagcacggta gcacacgcat
     9121 cggatcacga gcagcaaacg gaattgcacc ttccgaaata aagcacagtc ccagaaccag
     9181 agcggctttg ccaccttcct gctgcgcttt gtcgaatttg cgacgcgcca ccattgttgc
     9241 cagacccatt gccagcggtg gcaccatacc tgccgccata atcgccgcca tcgggccata
     9301 ggtttgagta ctcagcagac ccacaccgaa tgcgtacgct gctttgttta ccggaccgcc
     9361 catgtcagta cacatcatgc caccgaggat cgcccccagc agaacagcat tcgcagtccc
     9421 catggtctgc agccagtgag tcaacccatc gagaatgcca gcaaccggtt taccgatcag
     9481 gtaaatcatc gccagaccga ccaccagact ggaaattagc ggaatgatca ggatcggttt
     9541 cagtgcctcc atactctgtg gcagtttcag ttgcgtactt attaacttcg caatgtaacc
     9601 cgccaggaag cccgcaataa taccgccaat gaagccagaa ccggtgctaa ccgccagcat
     9661 accgccaatc aggcccggcg tgagacccgg acgatcggca atggagaagg caatataacc
     9721 tgccagtacc ggcaccatca gcgcaaaggc tgaaccacca ccaatctgca tcagcgccgc
     9781 tgccaacgta cccggctctt taaacgcttc gataccaaaa gcaaaagaaa gcgcgataca
     9841 cagaccacct gcaacaacca tcggcagcat gtaagagacg cccgtcagca agtgacggta
     9901 agcgcctgca ctctctttct taccttcagt ggtcgctgtt tgagctttgc ccgctggttc
     9961 atacggcgtt gcttcagcaa ccgctttatc cagttcctgc gcagttttct tcagcgccag
    10021 accggtagag gtgcgataca tcggtttacc agcaaatttc gccagatcca cttcgatatc
    10081 tgccgccaca atcaccagat ccgctgctgc gacttcttca ggggtgattg cattacccgc
    10141 gccaacagaa ccacgggttt caactttcac ccaccagcca cgttttttcg cttcggtttc
    10201 aatggcttca gccgccataa aggtgtgtgc tacgccagtc gggcaagcag tcaccgcaac
    10261 tacgcgtttc ggaccgctgg cagcaaccgg tgctgtcgca gcgaccggcg cagtgtaagg
    10321 tttcgcatgg cctttggctt cactcaggaa cagctcaggg tgcgcaactg cccgggaaat
    10381 atcacccagc cagacatttt taccgttcag cgcgctgtcg ttcgggatgg aatcaccgag
    10441 aacaatcgcc atttcagcgt cgttcggatt gtcgatgatt tccagttttg cttttcgcgc
    10501 cgccgcgccc agcagggtct tcgccatata ggcgcgtgcc tgaccgagat tagcgtcaat
    10561 aatcagcagc gttttcatta tgcctctcct gctgtcagtt aaaaggttgt aagtcgacgc
    10621 gcgccatcat tgcggccaac tgcggacgat cggtaatacc cacattgctt tgacttaccg
    10681 ccagggctgc aacagctgtc gccagacgca gtgtgtgttc actggattca cgcatcagca
    10741 agccataaat caggccacca accatagaat cccctgcgcc aacggtgctt acgacatcga
    10801 ctgacggtgg tttggcgatc cattcgccgg aggcattaac ccaaagcgcg ccttcggcac
    10861 ccagtgaaat aacaacatgc gcaatacctt gttcacgtag cgcatgtgca gcttcaatca
    10921 catctttcat ttcaggcagt ttacggcctg cccagatttc caactcacgg cggttaggtt
    10981 tcaccagcca cggtgccgct ttcaaacctg ctactaacgc ttcacggcta ctatcaaaga
    11041 taatgcaagg acactgacta cgcaggcgag tcatccagtc ggtgaacgct tccgggctga
    11101 cgcctgacgg taagcttccg ctgacacaga ccatatcgaa ctgaccgagc cagctcagag
    11161 aatcagtcac aaagcgttcc cagtcggcgg gggtgacttc aaaacccgag aagttgaagt
    11221 cggtcacttc gccgtctttt tccgtcagct taacgttaat tcgggtgcgc ccctgtacaa
    11281 cctggaaacg gttggcaatg cccagctcgc tgaacagttg ctgaaaacca tcctgattgt
    11341 ctttacccag gaagccgcca acggtgacat caattcccag gtcttttaat actttggcta
    11401 cgttgatgcc tttacccgcc gcatgcagac cagtggtttt caccaggttc acttcgccgc
    11461 gttcaatttc cgggcagaaa ccaacaaggt cataagccgg attaagggtg atcgtagcaa
    11521 cacgtctgct cattatgcgc cctccccaag accagcagcg atagcgtcgc caatggcttt
    11581 cagcgcctgt tcagcatctg caccctgggc ggtaaagcgt aggcgatgac ctttcttaac
    11641 gccaagtgcc acaactttca tcagactacg tccgtttgcc ggtttgccgg tgccatcaag
    11701 gtttgtcacg gtaatatcac tgttaaattg tttaatggta ttgaccagca tggtacctgg
    11761 acgagcatgc aggccgtgtt cattgcgcac cacaaactcc gcgcttaaca cgtcgtcggt
    11821 cggcgcatca tcgctggtca gcagcgccag caacgttgcc gcatccgctt tcagcaagcg
    11881 gtcagcttta ttatcgagca acaaatcagc gagacgctta agaaccgcga tgggctgatc
    11941 gtcattcatc gccacactca ccagcatggc tgccgtttcg ccgtccacat caaaagcatt
    12001 tgccgcacgg cttaccgcaa tcgcgctacg cagattgcct tcggcgctat cgctcagcca
    12061 gataccctgc ccgaggttca gcggttgttc attgatggct ttggtgacga aagtggcgtc
    12121 aactgccccc gcttctttca gacgcgcagc gttcagcgcc tgaagagtca gcagatcgct
    12181 ggcgacgata tccagcgtca gcatttcgtt gtcgagcttc agctgctcac tctgcttttc
    12241 gcccatcagc aatgcgcgaa gttcttctgc tgttgttgct gacttcagtt gttcagcaac
    12301 ggaatcatcg ctcagtacgt gggtcagctg gcgtagcagg cccagatgtt catccgagct
    12361 ggcagcaata ccgatcgcca cgtacgcgac ctggccgtca ccccaggtga cgccttccgg
    12421 gaactgaaat acctgaacgc cggttttcag cacctgatcg cgggtgtcgg tagtgccgtg
    12481 tggaatagca ataccattgc cgaggaacgt tgaggtttgc tgttcgcgcg ccagcatgcc
    12541 attgacgtag ccttcagcta cattaccggc ctgcaccagc gccgcagcga cctggcgaat
    12601 cgcctcttct ttgtctccgg ccttttcgcc cggatggatg tcctgtacgg ataactggaa
    12661 catagttctc ctctcttgct gaattgaaac gattcagctt ctatgagaaa aaagcgccaa
    12721 cctggcttag ggttaaagac aagatcgcgc tgaaacgttt caagaaagca taatactttt
    12781 gtttcagcac gcaaggaaag ccgtaaagtt atgagcaaaa gtttgatgtg ctgcacaatt
    12841 ttttgtcatt tttcctaatt gctgatggga aaatccgtta tcagcaattt catttcagca
    12901 gcgtcagcag ccaatcagca acagtgaaat ggcattttga ttattcgtgg cgcttttgat
    12961 ttcgctgtct gtttattttc tgacaagcag cgtaaactcc gcgtcttcct cttccagtga
    13021 tcgaccagca tgcataactc ccccgcagtc accagcgcga aatcgtttga cctgacctcg
    13081 atggcgtttt taatcgttgc ctttctcacc ggtattgcgg gcgctctgca aaccccgaca
    13141 ctcagtattt ttcttactga tgaagtacat gcccgtccgg cgatggtggg attcttcttt
    13201 accggcagcg cagtcattgg gattctggtg agtcagtttc tcgccgggcg ctctgataag
    13261 cgcggcgatc gcaaatcgct gattgtcttt tgctgcctgt taggcgtgct ggcctgcacc
    13321 ctttttgcct ggaatcgcaa ctactttgtt ttgctattcg ttggcgtctt tcttagcagc
    13381 tttggctcaa ccgctaaccc gcaaatgttt gcccttgccc gtgaacatgc tgacaaaacc
    13441 ggacgtgagg cggtgatgtt cagctctttt ttacgcgctc aggtttcact ggcatgggtc
    13501 attggcccac cgctggctta tgccttagcg atgggtttca gctttacggt aatgtatctg
    13561 agcgcagcgg tagcgtttat tgtttgcggc gtgatggtgt ggctgttttt gccgtcgatg
    13621 caaaaagagc ttccgctggc gaccggcacg gttgaagcgc cgcgccgtaa ccgtcgcgat
    13681 acgttgctgc tgtttgtcat ttgtacattg atgtggggct cgaacagcct gtacatcatc
    13741 aacatgccgc tatttattat caacgaactg catcttcccg agaaactggc cggtgtgatg
    13801 atggggaccg ccgcagggct ggaaatcccg accatgttga tcgccggata ttttgccaaa
    13861 cgtctgggta agcgcttctt aatgcgcgtt gctgccgtgg gtggcgtctg tttttacgca
    13921 ggaatgctga tggcgcattc acctgtcatt ctgttgggct tgcagctgct aaatgctatt
    13981 tttattggca ttctgggcgg catcgggatg ctctattttc aggatttgat gcccggtcag
    14041 gcaggttcag ccaccacgct ctataccaac acgtcgcgcg tgggctggat catcgcagga
    14101 tcagtggcgg gcatcgtcgc cgagatctgg aattatcacg ctgtgttctg gtttgcgatg
    14161 gtgatgatta tcgccactct gttttgctta ctgcgaatta aagatgttta aggcgcggtc
    14221 aacatttcca gatcgataag ccaggtcatt gcctgctgac gcgagttacc gcacatttca
    14281 gcgctggctt gtaagcctgc acagactttc ggtcgcagtg gcgaggtgaa aattttacag
    14341 cgttgctgtt catcaagctg aatgcagggc gtattggcgg gcttgccatc tggcataccg
    14401 ggaatagggc tggaaattga aggggcagtg caacacgccc cgcaacccgg acggcattcc
    14461 atacgttgtt ctcttaaggt taaccaccgg attgatgagc gcgcacagta ccaccttttt
    14521 tgcaccagca aaagtgcgaa taccacttgc ccgaaaggcc cgtcgcgagt actttgtcgc
    14581 gatatttttg acatttttga ctacaggaat ttttcgatgc caagagcgaa cgaaattaaa
    14641 aaaggtatgg tactgaatta caacggcaaa ctgctgctgg ttaaggacat tgatattcag
    14701 tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc gtttttctga tgtccgtacc
    14761 gggctgaaag tagaagagcg tttcaaaggt gatgatatcg ttgacaccgt gacgctgacc
    14821 cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat atgtctttat ggataaagaa
    14881 gactataccc cgtatacctt caccaaagat cagattgaag aagagttgct gtttatgcct
    14941 gagggcggca tgccggacat gcaggtgctg acctgggacg gtcaattgct ggcgcttgag
    15001 ctgccgcaga ccgttgatct ggaaatcgtt gaaactgcac caggcatcaa aggggcatct
    15061 gccagcgccc gtaacaaacc ggcgacattg agcactggtc tggtgattca ggtaccagaa
    15121 tacttaagcc cgggcgaaaa aattcgtatc catatcgaag aacgccgtta tatgggtcgt
    15181 gctgactaac ttcagccgta tgcagaaaag ggatagctca ggctgtccct tttttaattt
    15241 attataccaa tcttctattt tgcgcttcct gaacacctta cgcccgcaat tttcactcgt
    15301 tgattgatcg ccctcacact tcatcgcatt aacaatccag accaatttca attgttgtca
    15361 tataacttta cactgttatt gttaattaat cgttactaag acgtgactcc tatgaataca
    15421 atcgcctccg ttacgctccc gcatcatgta cacgctccac gctacgatcg acagcagttg
    15481 caatcacgta tcgttcattt tggctttgga gcctttcacc gcgcacatca ggcattactg
    15541 accgatcgtg tgctgaatgc ccagggcggc gactggggga tctgtgaaat cagcttgttc
    15601 agcggcgatc aactgatgag ccagctccgc gcacagaacc atttatatac cgtgctggag
    15661 aaaggtgcgg acggcaatca ggcgataatt gtcggtgccg ttcacgaatg tctgaatgca
    15721 aaactggatt ccttagcggc aattattgag aaattttgcg agccacaggt ggcaattgtt
    15781 tccctgacaa ttaccgaaaa aggctattgt attgacccgg ccaccggtgc actcgacacc
    15841 agcaatctgc gtattattca cgatctacaa aaccctgaag aacctcactc cgcgccaggt
    15901 attctcgtcg aagcactgaa acgccgccgt gagcgcggcc ttacaccgtt taccgtgctc
    15961 tcctgcgaca atattcccga caatggccat gtggtgaaaa acgcggtgct gggaatggca
    16021 gaaaaacgtt cgccagaact cgccgggtgg ataaaagaac acgtcagttt tccgggaact
    16081 atggtcgacc gcattgttcc ggctgcaaca aacgaatcac tggcggaaat cagccagcat
    16141 ctgggggtga atgatccctg cgcgattagc tgcgaaccgt ttatccagtg ggtggttgaa
    16201 gataacttcg tcgctgggcg tcctgcctgg gaaatcgcag gtgtacaaat ggtgaatgat
    16261 gtcctgccat gggaagagat gaaactgcgt atgcttaatg gcagccactc ttttctcgct
    16321 tatctgggtt acctctcagg attcgcccat atcagtgatt gcatgcagga tcgcgcattt
    16381 cgccatgccg ccagaacatt aatgctggat gagcaagcgc cgacactgcg gattaaagat
    16441 gtcgatttaa cacaatatgc ggataagtta attgcacgtt ttgctaatcc ggcactgaaa
    16501 cataagacca ggcaaatcgc gatggatggc agccaaaaat taccgcaacg catgctggca
    16561 ggtattcgca tacatctgag gcgcgaaacg gactggtcgt tgctggcatt aggcgttgca
    16621 ggctggatgc gttacgtcag cggcgttgat gatgccggaa atgccattga tgttcgcgat
    16681 ccacttagcg ataaaattcg tgaacttgtt gcgggcagca gcagtgaaca acgcgtaacc
    16741 gccctgcttt ccctgcgtga aattttcggt gatgatctgc cagataaccc gcattttgtg
    16801 caggccatcg aacaagcctg gcaacaaatc gcacaattcg gcgcgcatca ggcgctatta
    16861 aacaccctca aaatttaacg atttctgcgg ttaaagcgga tgaagctcgc cttcgtccgc
    16921 tctccccttc tcttttctgc cttttttagc caggattaac gctcagttaa cttaccagag
    16981 tattgcggag ccgttgtgac cagaaccaac ctcatcaccg gttttctcgg cagcgggaaa
    17041 actacgtcga ttcttcatct gttagcccat aaagatccca acgaaaaatg ggcggtactg
    17101 gttaatgaat ttggggaagt cggaattgat ggtgctttgc tcgccgatag cggcgcactg
    17161 ctgaaagaga tccccggcgg ttgcatgtgc tgcgttaatg gtttacccat gcaggtaggg
    17221 ttgaatacct tactgcgtca gggaaaacca gaccgcttgt tgatagagcc gaccgggctg
    17281 ggccatccga aacagatcct cgatctctta accgcacccg tctatgaacc gtggatagat
    17341 ctgcgcgcca cgttgtgcat tctcgatcca cgcctgttgc tggacgaaaa aagcgccagc
    17401 aatgaaaact tccgtgacca actggctgcc gcagacatta ttgtcgccaa taaatccgac
    17461 cgtgcgacgc ccgaaagtga gcaagcgcta gagcgctggt ggcagcaaaa tggtggcgat
    17521 cggcaattaa ttcacagcga gcatggaaaa attgacggtc atcttctgga tttgccgcgt
    17581 cgcaatttag ccgagttgcc cgccagcgcc gcgcattctc atcagcatag cgtgaaaaaa
    17641 gggttagcag cgttaagcct gccagagcat caacgctggc gtcgcagtct gaacagcgga
    17701 caagggcatc aggcctgtgg ctggatattc gacgctgata cggtattcga caccattggt
    17761 attctggaat gggcgcgact tgcaccggtg gaacgcgtca aaggcgtgct gcgtattccc
    17821 gaagggctgg tgcgaatcaa ccgtcagggc gatgacctgc acattgaaac gcaaaacgtt
    17881 gcgccaccgg acagtcgtat tgagctgatt tccagcagcg aagctgactg gaatgccttg
    17941 cagagcgcgc tgttgaagct tcgtttagcg actaccgcgt aaggttgcct gcgtttttca
    18001 gtaagataat tagagaaaat atgattaaaa atttgccgca aatagtgttg ttgaatattg
    18061 tcggcctcgc gctgtttctt tcctggtata ttcccattaa tcatggattc tggttgccga
    18121 ttgatgcgga tattttttat ttctttaatc agaaactggt cgaaagtaag gcctttttgt
    18181 ggctggttgc attgaccaac aatcgcgctt tcgacggttg ttcactgctg gcgatgggaa
    18241 tgttgatgct gagtttctgg ctgaaagaaa acgccccagg cagacgacgt atcgtgatta
    18301 ttggtctggt catgctatta actgcagtgg tattaaacca gctgggtcag gcattaattc
    18361 ctgtaaaacg ggccagccca acgttgactt ttaccaatat taaccgcgtc agcgaactgc
    18421 tctctgttcc cacgaaagat gcctcacgag atagctttcc cggcgatcac ggtatgatgc
    18481 tgcttatttt ttcggcattc atgtggcgtt atttcggcaa agttgcaggc cttatcgccc
    18541 ttattatttt tgtggttttt gcgtttccca gagtaatgat tggcgcacac tggtttactg
    18601 acatcatcgt cggttccatg accgtgatat tgatcggttt gccctgggtg ttgctgacgc
    18661 cattaagtga tcgattaatc accttttttg acaaatcact tcctggaaaa aacaaacatt
    18721 tccaaaacaa ataactcaca gtaattaaca tcatcaggtt tatttttata gtgaggataa
    18781 tcctgatgat gcgcctcctg ctttcatcca tcgagcgcaa aaatcattct ctaagtaaat
    18841 gaatggattg catgcgtttc actcaatcgt actttaattg accaatcccg ctgattaact
    18901 ttctgtatca ctttttctta taaaaaatca tgtaaaaccg ctcgccaaga ccgcaccaat
    18961 cgggtaatct cgaactcgtt ttgcctcggc ggtagattat cctcacagca tataattttg
    19021 tgcgttagtc cacagatttg gccttaagga attgtttcaa catgcccagg taattagtct
    19081 cgtgtcgctt ggcatttttt tataacgata tttgtcgtta aggacttcaa gggaaaacaa
    19141 acaacatggt caaatctcaa ccgattttga gatatatctt gcgcgggatc cccgcgattg
    19201 cagtagcggt tctgctttct gcatgtagtg caaataacac cgcaaagaat atgcatcctg
    19261 agacacgtgc agtgggtagt gaaacatcat cactgcaagc ttctcaggat gaatttgaaa
    19321 acctggttcg taatgtcgac gtaaaatcgc gaattatgga tcagtatgct gactggaaag
    19381 gcgtacgcta tcgtctgggc ggcagcacta aaaaaggtat cgattgttct ggtttcgtac
    19441 agcgtacatt ccgtgagcaa tttggcttag aacttccgcg ttcgacttat gaacagcagg
    19501 aaatgggtaa atctgtttcc cgcagtaatt tgcgcacggg tgatttagtt ctgttccgtg
    19561 ccggttcaac ggggcgccat gtcggtattt atatcggcaa caaccagttt gtccatgctt
    19621 ccaccagcag tggtgttatt atttccagca tgaatgaacc atactggaag aagcgttaca
    19681 acgaagcacg ccgggttctc agccgcagct aataaaccgt ttggatgcaa tcccttggct
    19741 atcctgacga gttaactgaa agcactgctt aggcagtgct tttttgtttt cattcatcag
    19801 agaaaatgat gtttccgcgt cttgatccag gctatagtcc ggtcattgtt atcttttaaa
    19861 tgttgtcgta atttcaggaa attaacggaa tcatgtttat acgcgctccc aattctggac
    19921 gtaagctcct gcttacctgc attgttgcag gcgtgatgat tgcgatactg gtgagctgcc
    19981 ttcagttttt agtggcctgg cataagcacg aagtcaaata cgacacactg attaccgacg
    20041 tacaaaagta tctcgatacc tattttgccg accttaaatc cactactgac cggctccagc
    20101 cgctgacctt agatacctgc cagcaggcta accccgaact gaccgcccgc gcggcgttta
    20161 gcatgaatgt tcgtacgttt gtgctggtaa aagataaaaa aacattctgt tcatctgcaa
    20221 ctggtgagat ggacattcca ctcaatgaat tgattccggc gctcgacatt aataaaaacg
    20281 tcgatatggc gatcttaccc ggcacgccga tggtgccgaa caaacccgca atcgtcatct
    20341 ggtatcgcaa ccctttgctg aaaaatagcg gcgtgtttgc cgctctgaat ctcaacctga
    20401 cgccttcact cttttatagt tcacggcagg aagattacga tggcctcgcc ctcattattg
    20461 gtaatactgc gctatctacc ttttcttcac gtttgatgaa cgttaatgaa ttaaccgaca
    20521 tgccggtccg tgaaacgaaa attgcgggca ttcctctgac cgttcggctt tatgcagatg
    20581 actggacatg gaacgacgtg tggtacgcat ttttactggg tggcatgagt ggaactttcg
    20641 ttggacttct ctgctattac ctgatgagtg tgcgtatgcg cccaggcaga gaaatcatga
    20701 ccgccatcaa gcgcgaacaa ttttacgtgg tatatcaacc ggtggttgat acacaagctt
    20761 tgcgggtaac gggcctggaa gtactgctac gctggcggca tccagtagca ggagaaatac
    20821 ccccggatgc cttcattaac tttgccgaag cgcaaaagat gattgtgcca ctgactcagc
    20881 acctgtttga gttgattgcc cgcgatgccg cagaattaga aaaagtactg ccggtaggcg
    20941 tcaaatttgg cattaacatt gcgccggccc acttgcacag cgaaagcttt aaagcggata
    21001 tccagaaact gctcacttcc ctgcccgcac accatttcca gattgtgctg gaaattaccg
    21061 agcgcgatat gctgaaagag cgagaagcca cacaactctt cgcctggctg cactcggtcg
    21121 gcgtagaaat tgctattgat gacttcggta ccgggcacag cgcgcttatc tatcttgagc
    21181 gttttacgct cgattatctg aaaattgatc gtggatttat caacgccatc ggtacggaaa
    21241 cgatcacttc acccgtactt gacgcggtgc tgacgctggc gaaacgtctc aatatgctga
    21301 cagttgctga aggggtcgaa acgccagaac aggcacgatg gctaagcgaa cgcggcgtta
    21361 atttcatgca aggctactgg attagtcgcc cgttaccgct ggacgatttt gttcgctggc
    21421 tgaagaaacc gtatacgccg cagtggtaag gtgtgcttac gtcccttatt attcatagtg
    21481 aaagcatgcc ggattgcggc taatgatgag taaaaggaaa tccgttgcag atgattgtgc
    21541 gcatactgct gctgtttatc gctctgttca cctttggtgc gcaggcgcag gctatcaagg
    21601 aaagctatgc ctttgccgta ctgggcgaac cccggtacgc atttaatttc aaccattttg
    21661 attatgtgaa ccccgccgcg ccaaaaggtg ggcaaataac gttgtctgcc ctcggcacct
    21721 tcgataattt caaccgctat gcactgcgcg gcaatccggg cgcacgcacc gagcagctgt
    21781 acgacacgct atttacgact tccgatgacg aaccaggcag ttattacccg ctgattgctg
    21841 aaagcgcacg ctatgctgaa gattattcct gggtggaggt cgctattaat ccacgcgccc
    21901 gttttcatga tggttcgccc attactgccc gcgatgtaga gtttactttt caaaaattta
    21961 tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg caccaccgtc aaagccattg
    22021 cgccgttaac cgtgcgcatt gagttagcta aacccggcaa agaagatatg ctgagtctgt
    22081 tttcgctgcc ggtatttcca gaaaagtact ggaaagatca caaacttagc gacccgctcg
    22141 ccacgcctcc gcttgccagt ggtccgtacc gcattacgtc ctggaaaatg gggcaaaata
    22201 ttgtctattc ccgcgtgaaa gattactggg cagcaaactt accggtaaac cgtggacgct
    22261 ggaatttcga caccattcgc tacgattatt acctcgatga taatgtcgcc tttgaagcgt
    22321 ttaaagcagg agcctttgat ttgcgtatgg aaaacgacgc caaaaactgg gccacgcgct
    22381 ataccggtaa aaatttcgat aaaaaataca tcatcaaaga tgagcaaaag aacgaatcag
    22441 cccaggatac gcgctggctg gcgtttaata tccaacgtcc ggtattcagc gatcgccggg
    22501 tccgggaagc gatcactctc gcctttgact ttgaatggat gaacaaagcg ttgttttaca
    22561 atgcctggag tcgcacgaac agttattttc agaataccga atatgcggcc agaaattacc
    22621 ccgacgccgc ggagctggtg cttctggcac caatgaaaaa agatctaccg ccagaagtct
    22681 tcacgcaaat ctaccagccg ccggtatcca aaggcgatgg ctacgatcgt gacaatctgt
    22741 taaaagccga caaacttctc aacgaagcag gctgggtgtt gaagggtcag caacgcgtta
    22801 atgtcacaac gggtcagcca ctcagctttg aattattgct tcccgcaagc agcaatagtc
    22861 agtgggtatt gccgttccag cacagcctgc aacggctggg tatcaacatg gacattcgca
    22921 aggtggataa ctcgcaaatc accaaccgca tgcgcagtcg cgactatgac atgatgccgc
    22981 gcgtatggcg ggcgatgccg tggcccagtt ccgatttaca gatttcctgg tcatcggaat
    23041 atatcaattc cacttataat gcccccggcg tgcaaagccc ggttatcgac tcgctgatca
    23101 accaaattat tgccgcgcag ggaaataaag aaaaattact accgttggga cgagcactgg
    23161 atcgcgtatt aacgtggaat tattacatgc tgccaatgtg gtacatggcg gaagaccgtc
    23221 tcgcctggtg ggataaattc tcccaacccg ctgtacgccc tgtttacagc ctgggtatcg
    23281 atacctggtg gtatgacgtt aacaaagcga cgaaactgcc gtcagccaga caacagggag
    23341 agtagatggg cgcttatctg attcgccgtc tgttgctggt gatcccaacg ctatgggcga
    23401 ttatcactat caactttttc atcgtgcaaa ttgcgcctgg cggtccggtc gaccaggcca
    23461 tcgccgccat tgagtttggc aatgccggag tattacccgg cgcaggcggt gaaggtgttc
    23521 gcgccagcca tgcgcaaacg ggcgtcggca atatcagcga cagtaattac cgtggcggac
    23581 gcggattaga tccagaagtg atcgccgaga tcactcatcg ctacggtttc gataagccga
    23641 tccacgaacg ttacttcaaa atgctctggg actacatccg ttttgatttt ggcgacagcc
    23701 tttttcgcag cgcctcggtg ctgacgctga ttaaagacag tctgccggtc tccatcaccc
    23761 tcggattgtg gagcacgctg attatctatc tggtgtcgat tccgttaggc attcgcaaag
    23821 ctgtttataa tgggagccgt tttgacgtct ggagtagtgc atttatcatc atcggctacg
    23881 ccattccggc ctttttgttt gccatcctgc tgattgtctt cttcgcgggc ggcagctatt
    23941 tcgacctgtt ccctctgcgc ggcctggttt ccgctaactt tgattcgctg ccgtggtatc
    24001 agaaaatcac cgattatctg tggcatatca cgctgccggt gctggcgaca gtgattggcg
    24061 gctttgcggc tctgaccatg ctgacaaaaa actcattcct tgatgaagtg cgtaagcaat
    24121 acgtggtgac ggcacgtgcg aaaggggtaa gtgaaaaaaa tattctctgg aaacatgtgt
    24181 tccgcaacgc catgctgctg gtgattgccg gttttccggc gacgtttatc agcatgtttt
    24241 ttaccggctc gctgctgatt gaggtgatgt tttcactcaa tggtcttggc ttactgggct
    24301 acgaagcgac cgtctcgcgc gattatcctg taatgtttgg taccttgtat attttcaccc
    24361 tgattggcct gctgctgaat attgtcagtg atatcagcta tacgctggtc gatccgcgta
    24421 tagattttga gggacgctaa tgccgcgact cagccccgtc aatcaggccc ggtgggcgcg
    24481 ttttcgccat aaccgtcgcg gctactggtc gttatggatt ttcctcgtct tgtttggttt
    24541 gagtttgtgt tctgaactta tcgccaacga taaaccgttg ctggtgcgtt atgacggcag
    24601 ttggtatttc ccgttgttga aaaactacag cgaaagcgat tttggcggcc cgctggcaag
    24661 tcaggctgat tatcaggacc cgtggctgaa acaacggctg gaaaataacg gctgggtact
    24721 gtgggcaccg attcgctttg gtgctaccag tatcaacttt gctaccgata agcccttccc
    24781 ttctccaccc tcccggcaaa actggctggg aacggatgcc aatggcggcg atgtgctggc
    24841 gcgcattctc tatggcacgc ggatctcagt tctgtttggc ctgatgctga ccctctgctc
    24901 cagcgtgatg ggcgtgctgg cgggggcgct acaaggctat tacggtggta aagtcgatct
    24961 ctgggggcaa cgttttattg aagtatggtc ggggatgccg acgctgtttt tgattatttt
    25021 actttccagc gtcgtacagc ctaacttctg gtggctgttg gcaattaccg tcttgtttgg
    25081 ctggatgagt ctggtcggtg tggtgcgggc ggagttttta cgtacccgta atttcgacta
    25141 catccgcgcg gcgcaggcgc ttggcgtcag cgatcgcagt atcatcctgc gtcatatgtt
    25201 gccaaatgcc atggtcgcta ccctcacttt tttaccgttt attttatgta gttcgataac
    25261 caccctgacc tcactcgatt tcctcggctt cggtctgccg ctcggttcac cgtcactcgg
    25321 tgaactgctg ttacaaggga aaaataacct tcaggccccg tggcttggga tcaccgcctt
    25381 cttatcggtg gcgatattgt tgtctttgct gatctttatt ggtgaagccg tccgcgactc
    25441 atttgatcct aataaggcgg tgtagcatga cgcaaactct gttagcgatt gaaaatttgt
    25501 cggtgggttt tcgccatcag caaaccgtac gtacagtagt aaatgatgtt tcactacaga
    25561 ttgcggctgg cgaaacgctg gcgctggtag gtgagtctgg ttcaggtaag agcgttaccg
    25621 cgctgtcaat tttacgcctg ctcccttgcc cgccggttga atatctctcc ggcgatattc
    25681 gttttcatgg cgaatcgctg cttcatgcca gcgatcaaac gttgcgcggt gtacgcggta
    25741 ataagatcgc catgattttt caggagccga tggtgtcgtt aaatccatta cataccctgg
    25801 aaaaacagct ttatgaagtg ctttcactcc accgcgggat gcgtcgggaa gcggctcgtg
    25861 gcgaaattct taactgcctt gatcgcgtcg gtatccgcca ggcggcaaaa cggctgacag
    25921 attatccgca tcagctctcc ggcggcgaac gccagcgggt gatgattgcg atggcgctgt
    25981 taacgcgacc ggaattatta attgccgatg aaccaaccac cgcgctggac gtctctgtcc
    26041 aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga gctgaatatg ggtatgctgt
    26101 ttattactca taacctcagc attgtcagaa aactggccca ccgcgtggcg gtaatgcaaa
    26161 acggtcgctg tgtcgagaaa aataacgccg ctacgctatt tgcctcaccc actcatcctt
    26221 acacacaaaa gctactcaac agtgaaccat ctggcgaccc ggtgccattg ccagaacctg
    26281 cctcaacgtt gctagatgtt gaacagcttc aggttgcctt ccccatccgc aaagggattt
    26341 tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat cagttttacg ctacgggcgg
    26401 gtgaaacact gggtttagtg ggcgagtccg gttccgggaa aagcacgacg ggacttgcgc
    26461 tgctgcgact gattaactct cagggcagca tcgtctttga cggtcagcca ctgcaaaatt
    26521 taaatcgccg ccagctgtta cctattcgtc atcgcattca ggtggtattt caggatccaa
    26581 actcctcact caacccacga ctcaacgttt tgcaaattat tgaggaaggc ttacgggttc
    26641 accagccgac gctttctgcc gcacaacgcg aacaacaagt gatagccgtg atgcatgaag
    26701 tgggattaga tcctgaaaca cgccaccgtt atccggcgga gttctctggt ggtcagcggc
    26761 aacgtattgc gattgccagg gcgttaattc ttaagccctc gctgatcatt cttgatgaac
    26821 caacatcatc actcgacaaa acggtgcagg cgcaaatatt gacgctattg aaatcattgc
    26881 aacaaaagca tcaactggcc tatttgttta tcagccacga tttgcacgtt gtccgcgcgt
    26941 tatgtcatca ggttatcgta ctgcgacaag gggaagtagt ggaacaagga ccgtgcgcgc
    27001 gcgtgtttgc cgcaccgcag caggagtata cgcgtcagct actggcgttg agctgacgct
    27061 taaaaaggat tgtagtttga aaagggttcg gcaattgcca caccaaaatt tttcaatcgg
    27121 catgttgcag caaattcgtc ctggcgattt acaaacaggc acggctcacc ttcacactcc
    27181 agcaccgaac atggaacttc gatgtcgctt aacgcctggc ttagtttgta catcaccgac
    27241 catgcattat caccatccgg gctaagcaat ttaagcgcca ccaggctgtt gtcaccgcac
    27301 ggtccatttt gcggaatcgg ttcaaccttc gaacctgcga aaccagcaga ccagcgatag
    27361 ttctgcggca gtcgatggac gattgagagt tgtaatgaag tcacttattt tccccggaag
    27421 tacatttact tcacaatttg tttacatcaa ttttaacaca tcatcaacaa tccgtcttta
    27481 tacagatcgt aaagattgat gcctcgcctg cgtggcctct atggctctga tttaaataat
    27541 atctgcgggc gtggtcacgt ttgcggggct atatgtaccc gtttctgcga tctaactcaa
    27601 cctttttaac tacaatgatg tgacttttta cataaattga ttttacataa aataaacata
    27661 tatcggggga atgatagatt tgtggttgac atgcatcaac aaaggaagcc ttttagcttc
    27721 ctcgttgtgc aatagatcac cgttttttcg gccgactggc gtacaaacag aagagaatgg
    27781 agctggttgc gcagaatgca attgaccaaa tcatcgacca tgcagagtta aaggtcgcaa
    27841 gagaaagtaa tgcgccaaca attgccccta tgccgaaacg gaaggttcct gccagcgaag
    27901 ataccgttcc cgccatatgg ggaaactcat caagaatgac cgccatcgca ttggatgaca
    27961 ccatcgacac gcagcccaca aacgccgcaa cgccaaccac cagcgaccag aatcccagcc
    28021 ccagcagtgc actgatgacc atccacgctg ccataataaa ctgtatccac aaccccgagc
    28081 ggaacatatt taacgcgcca atacggcgga cgaagcggct gttaaagatg gtcatcacga
    28141 acagaaaaag aatgtttagc gcaaagtaat aaccaaagtt ttccggcgcg acgtggttga
    28201 tttcaatgta gacaaacggt ccggcgctta agaatgagaa catcccggca aagctgaaac
    28261 cgctggccag catataactc aggacacgct tatgacggaa cagcgccgca aagttaccaa
    28321 tagtggtacg aatgtgaaaa ggctggcgac gctccggtgg taaggtttct ttgatcagaa
    28381 agaagatcat tgccgaagcc agaatcgccg ctaatgccag gatccagaag atgtaatgcc
    28441 agctcagcca caccagcacc cagccgccaa ctatcggtgc catcagcggt gcaatggtcg
    28501 tcaccagcat gacaaacgac atcattcgcg agaactcttc tttcgggtag atatcacgca
    28561 tcaaagcgtt gataaccacg ctggccgcag ccgcagccag cccgtggaag aaacgcatca
    28621 caatcagctg atcgatggtt tgcgccaacg cacacgccac cgcggcggcg gcaaacacca
    28681 gcgtaccgcc aagaaccacc ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta
    28741 actgccccaa cgcaaagccc agaatataag tactcagggt catctgcgta ctgcccgccg
    28801 gtacaccaaa ctgcgctgaa attaccggta gcgcaggcag atacatatca atcgacagcg
    28861 gcatcaacat ggccagcagg ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg
    28921 tggtcacaac gggctcctga aagtcattga aaagttagac gacgctggca atttcttctt
    28981 cagttaacgg acgatattca ccgggggcta aatcagcatc cagcgtaatg ccgccaatac
    29041 gttcacgatg cagctcaacc acatggttac ccacggcggc gaacatgcgt ttcacctgat
    29101 gataacgccc ttcgctgatg gtcagacgaa cctgcgttgg ggtaatcact tccagcaccg
    29161 ccggcttagt gagatctttt tcgttatgca gctgcacgcc tttagcaaat tgctctgccg
    29221 tatcgtcagc tacgggtgat tccagcgtta ccagataggt cttctcgcaa tgatggcgcg
    29281 gggaagtaat gcggtgcgac cactgaccat catctgtcat tagtaccaga ccggtggtat
    29341 cgatatccag acgccctgca gcgtgcagtt tccacgctac cggttcatca agaaaataga
    29401 gcaccgttgg gtgatccggg tcgtccgtgg agcaaacata gccctgaggc ttattgagca
    29461 tgaagtaacg tggaccgtgt tgctgcgcca gcgggttgcc atcgtaagcg acatcatgtt
    29521 cagggagcag tttgaacgct gcattacgga cgatttcgcc atcgacggtg acacgattgc
    29581 cgcggatttc acgcccggca atagcacggc taacgccgag ttgctgtgcg ataaatttat
    29641 caagtcgcat gtgtgtgatt ttgcctgtaa aaaacggagg tcgggtaatg acccgaaaat
    29701 ctgaactgtt gtctgcccag tatagcggtc taataacttc cctcaaggga aaaagatccg
    29761 tggcatacta ttagcagaat aatctaccta cgcgagacca tgatttttac actccgccca
    29821 tatcagcaag aagccgtgga tgccacgctc aaccattttc gtcgtcataa aacccctgcc
    29881 gtaatcgtgc tgcccaccgg tgcaggtaaa agcctggtga tagcggaact ggcgcggctg
    29941 gcgcgtggtc gcgtgctggt tctggcacac gttaaagaac tggtggcgca aaaccatgca
    30001 aaatatcagg cgctggggct ggaagccgat atttttgccg ccgggctaaa gcgcaaagag
    30061 agccacggta aagtggtatt tggcagcgtg cagtcggtcg cccgtaatct tgatgccttt
    30121 cagggtgaat tttcgctgtt gattgtcgat gaatgccatc gtattggtga cgatgaagag
    30181 agccagtatc agcaaatcct cactcacctg acaaaagtga atccccactt acgcctgctg
    30241 gggctgactg ccacgccttt tcgactgggc aaaggctgga tttatcagtt ccattatcac
    30301 ggcatggtac gcggcgatga gaaagccctt ttccgtgact gcatttatga gctgccgctg
    30361 cgttatatga ttaaacacgg ctatctgacg ccgccagaac ggctggatat gccagtagtg
    30421 caatacgatt tcagccgctt gcaggcacag agtaacggac tgttcagcga agccgatctc
    30481 aaccgcgagc tgaaaaaaca gcaacgcatt acgccacaca tcatcagcca gattatggag
    30541 tttgctgcaa cgcgcaaagg ggtgatgatt ttcgccgcga cggtcgaaca cgcaaaagag
    30601 attgtgggat tactgcctgc cgaagatgca gcactgatta ctggcgacac acccggctct
    30661 gagcgcgatg tgttaattga tgattttaaa gcccagcgtt ttcgctatct ggtcaacgtc
    30721 gcggtactga ccaccggatt tgacgccccg cacgtcgatc ttatcgccat tctgcgccct
    30781 accgaatcag tgagtcttta ccaacaaatt gtcgggcgcg gtctgcgcct ggcccccggc
    30841 aagactgatt gcttaattct tgattatgcg ggtaaccctc acgatctcta cgcgccggaa
    30901 gttggtacac cgaaaggcaa aagtgacaac gttccggtac aggttttctg ccctgcctgc
    30961 ggttttgcca acaccttttg ggggaaaacg actgccgacg ggacattgat tgaacacttt
    31021 ggtcgtcgct gtcagggatg gtttgaagat gacgacggac atcgcgaaca gtgtgacttc
    31081 cgtttccgtt ttaaaaattg cccgcaatgt aacgcagaaa atgatattgc cgctcgccgc
    31141 tgccgcgaat gtgacacggt actggttgat ccggatgata tgttaaaagc ggcgctacga
    31201 ctgaaagatg cgctggtatt acgctgtagc ggcatgtctt tgcagcatgg acacgacgag
    31261 aaaggcgaat ggttgaaaat cacctattac gatgaagacg gcgcggatgt gagtgagcgt
    31321 ttccgtctgc aaacgcccgc ccagcgtacc gccttcgagc agctttttat ccgcccgcat
    31381 acgcgcacac cgggcatccc tctgcgctgg atcaccgccg ccgatatcct cgcccagcaa
    31441 gcattactgc gacacccgga ttttgtcgtc gcccgcatga aaggtcagta ctggcaagtg
    31501 cgtgaaaaag tgttcgatta cgaaggtcgt tttcgtcggg cgcacgaatt acgcggttaa
    31561 tggtagtttt gattgatgta taaggcgatt gagtatagaa tctcgcccgc ttttgcatac
    31621 gcaaagcaga tcacttacct gttgctgggt cgcctgtagc aggattaatt taagagagaa
    31681 agaaatgttt actatcaacg cagaagtacg taaagagcag ggtaagggtg cgagccgccg
    31741 cctgcgtgcc gctaacaagt tcccggcaat catctacggt ggcaaagaag cgccgctggc
    31801 tgtcgaactg gatcacgaca aagtcatgaa catgcaagtt aaagctgaat tctacagcga
    31861 agttctgacc atcgttgttg acggtaaaga aatcaaagtt aaagctcagg acgtacagcg
    31921 tcacccgtac aaaccgaagc tgctgcacat cgacttcgtt cgcgcttaat tgctgaataa
    31981 gttgtaaaaa accccgctcc ggcggggttt tttgtctctg caaattatgc ctgattacgg
    32041 tattgctatt ttttgcaggc cagataaggc gttcacgccg catctggcat tcagaacaca
    32101 aaaccgatta attgccgcca gacgtccggc gctgcaattg atcgcgcaag ttcggcggcg
    32161 tgcctttaat ggtcagggta tcggttgctg gatcccagaa gatgcgttca cctaacagca
    32221 tcgcatcaaa gttaatagtc aagccaccgc cgctaccggc aaacttcgtc aactgacgca
    32281 gtgtgctacg gtctgccggg aagctctctt ccagttcgta gcctttttca gccgcaaact
    32341 ccgtgaagct cacttcgctg acgcctgcca gctctttcga cagcgattcc agttcaatct
    32401 cttcacctgc ctgcagctgc tcattacagt agctataaac ctgctggcgc acgttctgtc
    32461 gctctgcttt atccagttgt gcttcggcag tgaaatcatc gaccgcttgc aacagcccac
    32521 ggttttgcgc tttcgcgttt aacccttcgc tggcaccgag gaaatccata aagaaatccg
    32581 ccactttgcg ccctactcgc cctttcagga aagtgagata gcgggtcgac tctggattgg
    32641 tttcccactc ggttaaatcg atacgcgcaa caatatccgc atgattgata tcaagataat
    32701 gggttgggtt gatgtcgaga ttttcgttaa cacgcatact gctcaggttg ctcagaactg
    32761 ccaccagcag atactccacc gccagatagc gatagtggca aaacagcacg aatccaccgt
    32821 cagcaaaagg atatttcgct aattcgtcac gcaggcgacc ggttgccgcg cggctaaatg
    32881 ccagaaagtc ctcttcgccc tgacgctgta agcgcagcgt ctgcgccagt tcactctctt
    32941 cgctaaacaa gccgtaagct ttatttttgg cgctatagac ccgatgcagt tctgccacca
    33001 tctcaacgac ggtttccgtc ggttccagca atgaatcgcg caacaccagc tcaaggttct
    33061 gctcatcacg cttgataagc tggtgcagag caatctggtt gatatccaga ctcatgataa
    33121 actctccttt aagaccgggc ggtattcaac caccgccggt taagcgacgc aataaaagat
    33181 ggcggtcatt ttacagaagg cggcgtaacg agaatgattt aacgcataaa aaagcagaaa
    33241 aaaaaccgtt gctacggtaa tatgttgccc tttcatcaac taactgattt cgatttatgc
    33301 cacaaatttc ccgctacagt gatgaacagg ttgaacaatt gctcgcagag ctgctcaacg
    33361 tactggaaaa acataaggct ccgaccgacc tttccctgat ggtgttaggc aacatggtca
    33421 ctaaccttat caacaccagc attgccccgg cccaacgcca ggcaattgcc aactcttttg
    33481 cccgcgcctt acagtcctct atcaccgaag acaaagcgca ctaagggaaa cagataacag
    33541 gttatggtaa ctcatcgtca gcgctaccgt gaaaaagtct cccagatggt cagttggggg
    33601 cactggtttg cactgttcaa tattctgctt tcgctcgtca ttggcagccg ttacctgttt
    33661 atcgccgact ggccgacaac gcttgctggt cgcatttatt cctacgtaag cattatcggt
    33721 catttcagct ttctggtgtt cgccacctac ttgctgatcc tcttcccgct aacctttatc
    33781 gtcggctccc agaggctgat gaggtttttg tccgtcattc tggcaacggc gggaatgacg
    33841 ctattactga tcgacagcga agtctttact cgtttccatc tccatcttaa tcccatcgtc
    33901 tggcagctgg ttatcaaccc agacgaaaat gagatggcgc gcgactggca gctaatgttc
    33961 atcagtgtgc cggttatttt attgcttgaa ctggtgtttg cgacgtggag ctggcaaaag
    34021 ctgcgcagcc tgacgcgtcg tcggcgcttc gcgcgcccgc tggccgcatt cttatttatc
    34081 gcctttatcg cctcgcatgt ggtttatatc tgggccgatg ccaacttcta tcgcccgatc
    34141 accatgcagc gcgctaacct gccgctttcg tacccgatga cggcacgacg ttttcttgag
    34201 aagcatggcc tgcttgatgc gcaggagtat caacgccgtc ttattgagca aggcaatcca
    34261 gacgccgttt ccgttcagta cccgttaagc gaactgcgct atcgcgatat gggcaccggg
    34321 cagaatgtgc tgttgattac tgtcgatggc ctgaactact cacgcttcga gaagcagatg
    34381 cctgcgctgg caggttttgc tgagcaaaat atttcgttca cgcgtcatat gagctccggc
    34441 aacactacag acaacggcat ctttggcctg ttttatggca tctcgccaag ctatatggac
    34501 ggcattttgt cgacccgtac gcctgcggcg ttaattactg cgcttaatca gcaaggctat
    34561 cagctggggt tattctcgtc agatggcttt accagcccgc tgtatcggca ggcattgttg
    34621 tcagatttct cgatgccgag tgtacgcacc caatccgacg aacagaccgc cacgcagtgg
    34681 atcaactggc tgggccgcta cgcacaagaa gataaccgct ggttctcgtg ggtctctttc
    34741 aatggcacaa acattgacga cagcaatcag caggcatttg cacggaaata tagccgggca
    34801 gcaggcaatg tcgatgacca gatcaaccgc gtgctcaatg cactgcgtga ttctggcaaa
    34861 ctggacaata cggtggtgat tatcactgcc gggcggggta ttccgctgag cgaagaggaa
    34921 gaaacctttg actggtccca cggtcatctg caggtgccat tagtgattca ctggccaggc
    34981 acgccggcgc agcgtattaa tgcgctgact gatcataccg atctgatgac gacgctgatg
    35041 caacgcctgc tacatgtcag cacacctgcc agcgaatatt cgcaaggtca ggatttgttc
    35101 aaccctcaac gccgccatta ctgggttacc gccgcggata acgatacgct ggcaattacc
    35161 accccgaaaa agacgctggt gctgaacaat aacggtaaat accgcactta caacttacgt
    35221 ggtgaaagag tgaaagatga aaaaccacag ttaagtttgt tattgcaagt actgacagac
    35281 gagaagcgtt ttatcgctaa ctgattaatt ataaatcagt tagcaaaata tcttacttgc
    35341 aatcggtgtg gaaaacggta gtattagcag ccacgagtcg gcacgtagcg cagcctggta
    35401 gcgcaccgtc atggggtgtc gggggtcgga ggttcaaatc ctctcgtgcc gaccaaaaat
    35461 cccaagaaaa ccaaccaatt gcggttggtt tttttatatc tgcatttaat tcgataaaca
    35521 gacagcgaca catcacggcc tgtttatttt ctgttatcag aacgtccaga ctacacccgc
    35581 ctgagttgtg ctgctttctt cacgggagaa acggctgtcg ataccaagcg ttagccccgt
    35641 caatgttttc tcaaagccag cgcctgcatc agtggtcacg ttgttgcggc tgttaattgc
    35701 cgaactccac atcgccgtat cgtaattaac gccttttacg ctaccaagac gcccacgcac
    35761 ggtgtccagt tctgcatcaa ataccagcgg ttgtgctgcc gccatattca gcacatccgt
    35821 ggttgaaggg ggcatttgta cgcgattctc tgtgagactc cagctatggt tgccattatc
    35881 cagcaaggta tattcatacg tactgatatc aacaacgctt ccggcattgc ccaacgtaaa
    35941 tgccgcatca ccgcgcccgt tgttaccagt gtaaggttat ctcctgctgc cgggctggca
    36001 ccggtgtccg tcacgaatat tttgaaatca cctgttgctt taacctccag tagccctccc
    36061 ttttctataa cggcaaagac ggcaatccca tctttttcaa caattaagcg ccctccttta
    36121 atcgtgggat cagtcgccgc gccataaacc tgtagcactc cattattgat cgtagtctgg
    36181 tttaccgcgc cacccatttg aacaatttga taaccatcat ttaatactga gtattctgcg
    36241 gtgccattta tttcaacgta ctgatatccc ccgttaattt cagtattact acttacacca
    36301 aattcttata tatgctgttc tccgccagga ttgattttcg tattattggc ggccccataa
    36361 accagctgga tatctttttc caggacaaca ccatcaactg tctcaccata ctcaacagtt
    36421 gatgctaatg ctatgggtga atataacgct gtagccacca gaacagatat tgcggaacga
    36481 caaagagaaa cagaaccaga ttggtgcatt aagctttcat cctatgaaat taattgctgt
    36541 taaaagcatt ggggacagaa aatacccata gctccatacc cggagtcagt tttaaaaaac
    36601 tgtttaaaga aatgcacaag tattgtgatt gattttttag ttgtttttcc tgatgagaag
    36661 ctgatgcaaa attccgtctt tataatgaag atgatgccaa agcgaacgac aaagttgtag
    36721 ttttcactac atgtccatac ataaaatggg gtaacattca cgcgcctggt agcgttacca
    36781 acgctacgct caaacataat gattctaata aaacctcagg agactactat gcctgaagcc
    36841 acgccttttc aggtgatgat tgtggatgat catccactta tgcgacgcgg tgttcgtcag
    36901 ttactggagc ttgattctgg ctttgaagtg gtcgccgaag cgggcgacgg cgcaagcgcg
    36961 atcgatctgg cgaatagact ggatatcgac gtgatcttgc tggatctcaa tatgaaaggc
    37021 atgagtggcc tggataccct caatgccttg cgcagggatg gcgttaccgc gcaaattatt
    37081 atcctgaccg tatcagatgc ctccagcgat gtctttgcac tgatagacgc aggcgcagac
    37141 ggttatctgt tgaaagacag cgacccggaa gtgttactgg aagcaattcg tgccggagcg
    37201 aaaggcagca aagtctttag cgaacgcgtc aatcagtact tacgtgaacg tgaaatgttt
    37261 ggcgcggaag aagacccctt cagcgtgctg acagaacgcg agctggatgt tctgcacgag
    37321 ctggcacagg ggctgtcaaa taaacagatt gcctcggtgt tgaatatttc cgagcagaca
    37381 gtaaaagtcc atattcgcaa tctgctgcgt aaactcaacg tccgctcacg cgtggcggcc
    37441 accattctgt tcctgcaaca acgcggggca caataaaaat agcccgatgg catcaccatc
    37501 gggccacttt ttatttactc tcctgcagcg acaaatgttg catcgcctgc gcgatactac
    37561 gttcgatcac cgcacggcga gtatcgttgg caggtaagag tttcaacatc atctcccacg
    37621 cggcaacggc ttcgccaaat cgttgctgct caaaggcatt aaatgcatac atgcttaaca
    37681 cacggatatt gctatggtcc gttctcacca actggcgcag cagttcgccg ccgaggcggt
    37741 tgtcgttggg atcagatgaa cgtgtcaacg cttcagcgta tcccagtgca gcatcgctgt
    37801 ttttcggatc gaggcgatac gcagtggcgt atgcatcggt ggcgatactg gcgttaccca
    37861 gcgccatgcc aacgcggccc aacataatcc agccttctat atctcccgga tttttttgca
    37921 gttgagtacg catccccagc gcaaggcgcg acatctcttc ttcgttgagc ggatcggctt
    37981 tcggatccag cgccctgtcc agtaacgccg gagcctgtgc cgtggcctgc tgccagattt
    38041 tcacctgctg ataattgcca gtctggtagt agctgacgcc agccacaatt aacgccacca
    38101 caatacccgg cagataaaca acatatccgg cacgcttacc ttccggcacg ctttgttctg
    38161 gaaacgcttc cggcaccacg cgtacccgac gccgcgaacg ggcgtatatg acccaaccgc
    38221 caatgccaat agccactact ggcagcaccc acagcagcac ggtcagcggc gttaacggcg
    38281 gatcgtaagt gacgaagttg ccgtaacgcg ccaccatata atcgacaatc tctttcttac
    38341 ttttgccttc ctgcatcagt tcatacactt tctggcgcag gtcggtggca atcatcgagt
    38401 tggaatcggc aatgctgttg ttctggcatt tcgggcagcg cagttcttca gtgagctgac
    38461 ggaactgttg ttcctgtgct tcatctttaa actgcaacac gtcgatggtc gccagcgctg
    38521 agccggagat catcagcatc agcacgccca ataaaaacct cattgtgcgg cctccttact
    38581 gtatttctcc cacagtggct tgatctcttc ttcccagacg cgaggattga gatcgcccgc
    38641 atggcgatag cgaatgatgc cgttgccgtc aataaggaac gtttctggcg cgccatagac
    38701 accgagatcc agccctaaca tgccgtcgcc atcaaacagg cttagcgcgt aaggattgcc
    38761 cagctctttc agccagctga ttgccttctg gcgatcgtct ttatagttca tgccgaccac
    38821 gcggatgccc tgcgcagaaa gctgattcag atattgatgt tccgcacggc aggtcggaca
    38881 ccaggtcgcc cagacgttaa gcagcaccgg tttgccctga gtcagcacat ccgcctgata
    38941 aaactgcccc ggattgtcca gtgattcgag gcgaaacttc ggcactggct tgccaatgag
    39001 cgccgattcc agattggtcg gatcatcccc ttcggcatta cgcgccagct gccacagcag
    39061 cgccgcggca atcgccagga agataatcaa cggaattaac aataccttgc gcttcatgca
    39121 gcctccggcg cagttttctg cgggttcacg cgcttacgat agcgaggatc aaacagacac
    39181 agcagtccgc ccaacgccat catcagcccg cccgcccaaa tccagcgaac aaatggtttg
    39241 tagtaaagac gcacggccca cgcgccgttt tccagctctt caccgagtgc cgcgtacagg
    39301 tcacgcgtga tgccaccgtc aatcgccgct tcggtcatca tcgacccggc agtgttgtaa
    39361 taacgttttt ctgcatacag cactgtttcc ggcttgccat ctcgcgttac gccgatagtc
    39421 gccacaccgc cacgccagtt cggaccggtc acctctttga catcacggaa ggtgaagcga
    39481 tattcatgaa tatcgacgct atcgccggac ttcatgcgca catcacgctc aacgctatag
    39541 ttctgactaa aagcaatgcc aacaattgtc actgccagcc ccaggtgagc cgccaccatc
    39601 ccccaataac tgaaggtggt tttcgtgccg cgtgaaatac gcagcgcagc ttccgcaatt
    39661 gccagcaccg caatccagca ggccattgcc aggccgagca ctgtcatcgc cacaactttg
    39721 ctttcgaaca gccacggcaa cagcagcgac agcaccagcg tagagatgaa ggcgataatc
    39781 aacaaattgc ggattttacg tgggcgatcg cgtccccagc gcaccagagg gccgacacca
    39841 agcagtagcg caaacggcac catcaaccag gtaaacatgg tgttgaagaa cggttcgcca
    39901 atcgaaatac tgcccagtcc cagttgcttg tgcaccagcg gcagcaacgt ccccagcaac
    39961 accaccagca tcgcggcgac cagcaaaacg ttgttcgcta acagcaaaga ttcccgcgac
    40021 cacagcgcat tgtttacgcg tgagcgaact ttgtgtccac gcgcggcaaa cagcagcagc
    40081 gaaccgccaa tcaccagcac cataaaggcg aggataaaca taccgcgcgc cggatcagac
    40141 gcaaacgcgt gtaccgatac cagcacgccg gaacgcacca ggaaagtccc cagtagacac
    40201 aacgagaagg cactgattgc cagcagtaat gtccacgctt tgaagctggc gcgttgttca
    40261 gtgaccgcca gtgagtgcat cagcgcagtc cccaccagcc acggcataaa cgaggcgttt
    40321 tctaccggat cccagaacca ccagccgccc cagccgagtt cgtaataggc ccatgcggaa
    40381 ccgagcacaa tgccgagcgt caggaagatc cacgcagcca gcgtccacgg acgagtaaaa
    40441 cgcgcataag tgctgtccag acgcccactc aacaaagatg caatggcaaa agcaaacgcc
    40501 accgagaagc cgacgtaccc catataaagc agaggcggat ggaagatcag ccccggatcc
    40561 tgtaacagcg ggttaagatc gcgaccttca atcgggaagt tcggcaacgt gcgtgagaac
    40621 gggttagagg taaagagaat gaacagcaaa aagccgacgc tgaccatccc cattatcgcc
    40681 agtacgcggg ccacaatatc cagcggaata cgctgactaa aaatcgctac cgcaaaggtc
    40741 cagccgctca tcagcagcac ccacagcagg agcgagcctt catgcgcgcc ccaggtagcc
    40801 gccacgcgat accacaccgg aagctgggta ttggagttgc tggcaacata ggtgacggta
    40861 aagtcgttga ccacgaaagc attgaccagc accagaaatg cgccagccac agacataaac
    40921 agcagccagg caaacaagcg ggaagacgcc atcatgcgcg cgtccccgcg cgccacgccc
    40981 catagcggat acacggacag cagcagcgca attcctagcg ccagacacag cagcccgtta
    41041 ccaatttctg gcatcatgat gctgggtcct tataaacact cgccgggcga cggtggttag
    41101 cttccatcgc tttctcaact tctggcggcg tgtagttttc atcgtgtttc gccagcactt
    41161 ctttcgcgag gatatgattg cctttttcca gttcgccctg caccacaacg ccctgccctt
    41221 cacggaacag atccggcaaa atgccttcgt aagagacatc tactgaacct tcagcgtcgt
    41281 aaatggtgaa agttactttt agcgaattgg gatcgcgctg cacactcccc ggcatcacca
    41341 tcccgccaac gcgcagacgc tgaccgactt ccggcatctg ctgagtttca cgcttgccgt
    41401 agagaatctc ccccggcgta taaaagagat cgatattcga gcgcagcgca tatagcacca
    41461 gaccgatagt cagcgccagc cccgccagca cggcacaggc aatccacaag cggtttttac
    41521 ggcgaatatt catgcagcct cctgctgttg cgcagcacgt aaacgcgcct cacgcgcccg
    41581 ctgttgcgcc acgccacgca gaattgcgcg atgttgcatc accgagtgca cgaccaaaac
    41641 caccagcgga ataacggtca tcaccaccgc caaccagaca aaaaaggcgt aaccgcccat
    41701 tgcgaaaaat tcattccagg aagcaaatgc aggggtcatt tacggcctct tttcagtatc
    41761 agttcactca cccacggacg gcgtttttcc atcagcaaaa tcaaattacg catccgcatc
    41821 agcgtcagcg tggcagacag gagcaggaag ccaaaaatcg accagcgcag cggcgaacgc
    41881 atcgccggat cgatactttg ctgcatccgc gttgatccct gatgcagggt gttccaccac
    41941 tcaacggagt aatgaataat cggcagattc accacgccaa tcagcaccag gatacctgcc
    42001 gcacggccag ccagacgacg gtcgtcgaag gcgtgccaca gggcaatgac accaacatac
    42061 agaaacagca gcaccagttc agaagtcaga cgtgcatccc atacccacca ggtgccccac
    42121 atcggttttc cccatgcaga gccggtaacc agggcaataa aggtaaacac ggcaccaatg
    42181 ggggccatcg ccgccaccgc caggttggcc attttcatct gccagaccag gccaataaac
    42241 gctgccaccg ccattgatgc ataaatgccc atcgaccaga tcgccgcagg cacatgcagg
    42301 tagataatgc ggtagctatt tccctgctga taatcagccg gagcaaagcc gaacccccag
    42361 atccagccga cggtaagcac gaccacactg gcaattgcca gccacggtat aaaccagcca
    42421 cagatttgat acagccgtgg tgggatcgcc agttgatgca gtgttttcca catagtttcg
    42481 ataccagact cgaacaaaaa tcagtaatcc agcgttattg aatgctgatt cgtaacgctg
    42541 ccgccgtcgc aaaaggactt aatgtcgcgg tgcctgccag caacgcgcct aaaattgcca
    42601 gatacccatc aacgggcaaa tgcatagaag ccgcgtccat cgcggcggtg gcaaagatga
    42661 gtaatgggat agtcagcggt aacaccagta tgctgagcag cacaccaccg cgcttaagtc
    42721 ccactgtcag cgccacgccc ggcgcaccaa gaaagccaag cgtaggggtt cccagcagta
    42781 gcgttaacgc catcacttgc cagccataaa catccattcc cagtagcatt gctaccaggg
    42841 gcgaaaggat gagtaacggc agaccggtta ccatccagtg cgccatcacc ttcgccagca
    42901 cagcggcggg caagggtaac ggcaacaaca tcaattgttc aagactgccg tcctgcaaat
    42961 cgtcacggaa cagtcgctcg agtgccagca aggatgaaag cagcgcggca acccagataa
    43021 tgcccggcgc aatacgcgcc agcagttgcg gctccggacc gatactgagc ggaaaaaggg
    43081 taattacaat caggaagaac cacagcgggt tggcgatttc ggcgctatgg cgaaacgcta
    43141 cgcgcagctc aagacggaaa atgcgccaga acatcatgcg gcccccgttt gcgtcagtga
    43201 aatgcggcga attttacttt cagcaacgtt gagcggctgg tgggtagtca gaatcacaat
    43261 ccccccctgc tccgtatgct gcgccatacg ctgggtcaga cgatcgacgc cgttaacgtc
    43321 aatcgcggta aaaggctcgt cgagtatcca taacgtggca cgggtcaacc acagacgcgc
    43381 taaagcgacg cggcgttgtt gcccggcaga aagttgattt accggaatat cttcgaatcc
    43441 ggcaagcccg gcctgcgcca gggcttccag acattgtgcg gtatcgccat cgcgatgata
    43501 aaagtgcaga ttttctaacg ccgtcagccg ggttttgatc cccggctgat ggcctatcca
    43561 taacaggttt tgatggtagc tgtcgcgtac ctgatgcaag ggctgccctt gccagagaac
    43621 ctcgcctgca tcagggcgag acaaccccgt cagcaaacgg agaagcgttg tcttccccgc
    43681 gccgttgcta ccggtgattt gtacccactc tcctgcgttc agcgtaaatg ataaaccact
    43741 aaataaggtt cgttcatccc gctcgcaaag taactctctg gcttcaagca tacccacgca
    43801 ataaccctgt taaaaacctg gctcgacttc acgcatgtcg ggcagcttgt gcgctatccc
    43861 tttatggcaa tcaatacagg tttgcccatc tttcaccgcc tggtcatgca tcttcgcggc
    43921 aaccgatttc tgggcggttg tatccatata ctcgaagttg tgacagttac ggcactcctg
    43981 cgagttgttg tccttcatgc gccgccactc attctgtgcc atcgtcaggc gatgagcttc
    44041 aaatttctgc ggcgtgtcaa taacgcccaa aattttacca tacagctctt tacttgcttt
    44101 gagcttgcgt atcatctttg gcacaaactc gtgcggaacg tgacaatccg gacaggtcgc
    44161 acggacgccg ctacggttgt tgtagtgcac ggaatccatg tattcctgat acaccgtgtt
    44221 acgcatttcg tggcagctaa tgcagaactc ttcggtattg gctttttcca tcccggtgtt
    44281 aaagccgccc cagaagacga tgccgccgac aaaaccgatc aacagcagcg tccctagcgc
    44341 cagacggctg ggggtacgcc accatttcca caggcgctta atcagaccag gcttacggtc
    44401 agaatttccc ataataacct cttatttccc gtaacctttt gatggggtaa aggtattccc
    44461 cacgattggc gcgatatcgg cctgcggtac gtgacattgc agacagaaat aacgacgcgg
    44521 agccacttcc gcgcccactt tgccgtcgct gtccataaag tgagtaggac taatgcgcgg
    44581 cgcgccagtg gtgcgataac tttcgacacc gtggcactgc aagcagcggt tggtattggt
    44641 cgttacctga taaccttcaa cgctatgcgg gatcattggc ggctgattca catagttcag
    44701 cggcatccga tcctgctctt tcggcatccg aatggcccct tcctgcgtcc cggatacttc
    44761 cggcgactgg ctaaaatcga cgccattagc cgcccaaacc gcgccgctta ccaccagggc
    44821 cagcatcgcc gtccattgac acagcgcttt cttcaggtca tggcttttca tgatttcgct
    44881 cccgaactcc atcgtgtagt tattgtaaat acatcctcag aacagacatc cacgcagcga
    44941 ccgcacgtca tgcaatcgcg gctggtgacc tgcaccgggc tttgttcatc cagcaccggg
    45001 gcacgtagca catgcggttc cgggcaaaca tgaaaacaat ccatacagcg gttacatttc
    45061 tgccgatcgg tggccgcaac ggtaatcaca cctttgctac ccagcacgcc atacagtgca
    45121 ccaaccggac aaatgtgccc gcaccagccg tgttcaacga ccagtagatc aaataaaaac
    45181 agcgcgagaa taagcagcgc gccgctgccg aagcccatta ccaggctacg ccccatcaga
    45241 gaaacggggt tgatccattc ccagatgagc gtgccagtta aggccgatcc caccagaatg
    45301 accaccaaca gcacgtagcg aatatggcga gggatcgtcg cagactgatt gaggtcaaat
    45361 cgtctgcgta accaatttgc taagtcggta atcgggttca gcggacagac ccagctgcaa
    45421 aataatcgct ttcctgccag ggcgtagagc acggtgataa tcaccgcacc ggtcaacgcc
    45481 accgtggcag gcagatgacc gctggcaaga ctttgcagtg tcatcagcgg atcggtcagt
    45541 ggaacggtgt cgaataacag gctactgctg tagttgccgt gcaagatcca cacaccaaac
    45601 cacggaccgc tcaaaaacat ccccagcacg aagaactgac aaaggcgacg taacactaac
    45661 caacggtgac tgcgccacca gccttttttc tccagcgcct cgcgcccggc gtcacgttta
    45721 cgatttgcca ttgttcccct ccagccagcc gaagcggtaa tggtgaccta actccccttt
    45781 cgccagtgac agcggtaaca ccttgattgc cggttgttcc agcacgcaca ctttttcgca
    45841 cttaccgcag ccggtacagg cgtcgctgtg aaccgtcggc agaaagcggg cgtgcttacc
    45901 ggtacgcgtg ttgcgctcca gctccagggt gatggcctca tcaattttcg ggcattcgcg
    45961 ataacaaaca tcgcagcgca gcccctgaaa gttgagacag ttttcctggt ccaccagtac
    46021 cgccagcccc atccgcgcgt catcgatcga ttcgatctca cgatccagcg caccgctcgg
    46081 gcacactttg gcgcacggaa tgtcctcaca catttcgcaa ggaatatccc gtgcaacaaa
    46141 atacggcgta cccgccgaca ggccagaagc cagcgtcgcc agttttaagg tgtcgtaagg
    46201 gcaagcctga acacactgac cgcaacgcac acaggcactg gcaaaggcgt tctcgtttat
    46261 ggctccgggc gggcgcaacc gcacaccaga tgcgcgtgcg gtttgctgtt gtaaccccag
    46321 cgccacaccc acggcagcca gcccgcctgc tgtgcgaacg acatcgcgca gaaagcggcg
    46381 gcgaccattt tgaggtttcg ctgaccggga cataatggct tacaccttct ccagtttgac
    46441 cgcgcacttc ttgaagtccg tctctttcga gagcggatcg gtcgcatcca gcgtcagttt
    46501 gttaaccagc tgtgcggcgt cgaagaacgg catgtacacc aggccctgcg gcggacggtt
    46561 acgaccgcgc gtttcgacaa tcgagatcac ttcgccacgg cgagaaacca ctttcacttt
    46621 gtcgccacgg cgcagatcgc gcgctttcgc atccagcggg tgaataaaca gaaccgcttc
    46681 cgggaaggcg cggtgcagtt ccggtacacg gcgagtcata ctgccggtat gccagtgctc
    46741 caggacgcgt ccggtagaga gccacaggtc gtactcttca tccggtgctt ccgccgcagg
    46801 ttcgaacggc agcgcgaaga tcaccgcttt gccatccggt ttaccgtaga acttgtagcc
    46861 ttcgcccgct ttcacgtacg ggtcgttacc ttcgctgtaa cgccactgcg tttctttacc
    46921 gttcaccacc ggccagcgca gaccgcgtgc tttgtggtag tcatcgaacg gcgcgagatc
    46981 gtgaccgtga ccgcgaccaa accatgcgta ctcttcgaac agcccttttt gcagatagaa
    47041 gcccagctcg cgggattcat cgttcagctg atcttccgcc agttcggata ccgggaattt
    47101 gctcacttcc ggagtggcat acagaacgtc gtacagcgtt ttgccacgca gttctggttt
    47161 cttcgccagc agctcttccg gccacacttc ttcagttttg aagcgacggg agaactgaac
    47221 taactgccag agatccgatt ttgcttcacc cggtgcctgt acctgctgac gccagaactg
    47281 agtacggcgt tcggcattac cgtaagcgcc ctctttctct acccacattg cggtcggcag
    47341 gatcaagtcc gctgccagcg cactgactgt cggatacgga tcggaaacga tgataaagtt
    47401 gcgcggatcg cgccagcctg gcatacgctc ttcattaatg ttcggcccgg cctgcatgtt
    47461 gttggtacac atggtccagt aaacattcag tttgccgtct ttcagcgcac ggtcttgtgc
    47521 caccgcgtgc agaccgattt tcgccggaat ggtgccactc gggatattcc acttcttctc
    47581 gcaaatatca cgatgtttct cgttagtcac caccatgtcc gcaggcagac ggtgagcaaa
    47641 ggtgcccact tcacgcgcag taccacacgc ggaaggctgc ccggtcaggg agaacggacc
    47701 gcaacccggc tgggaaattt tgccggtcag caggtgcagg ttgtagacca ggttgttggc
    47761 ccacacacca cgggtatgct ggttgaagcc catcgtccag taggagatga ctttcttgtt
    47821 cggatcggca tacagctgcg ccagttgttc taactggtct ttcggcacgc cggtcatttc
    47881 ggcggttttt tccagcgtat attcagcaac gaaggctttg taatcttcaa agctcatcgg
    47941 ttcggaggcg tcagaacccg gattcttcgc tgctttttcc agcggatggg tcggacgtaa
    48001 accgtagccg atgtccgtcg cccctttgcg caggttaacg tgtttgctga agaagtcctg
    48061 atttatcgca ttgttttgaa tgatatagtt ggcgatatag ttcaggatca ccaggtcaga
    48121 ttgcggcgta aagatgatgc cgttatccgc cagctcgaag ctacgatgct ggtaggtaga
    48181 aagcaccgcc acggtaacgt tctggttaga gagacgacgg ttagtgatgc gcgaccagag
    48241 gatcgggtgc atctccgcca tgtttgcgcc ccacagcaca aacgcgtcag cctgctcgat
    48301 gtcgtcgtag cagcccatcg gctcatccat accaaaggta cgcataaagc caactactgc
    48361 cgacgccata cagtgacgcg cgttcgggtc gatgttgttc gaacggaagc ccgctttgaa
    48421 caacttggac gcggcataac cttcccagat agtccactga ccagaaccga acataccgat
    48481 cgattccggc cctttttctt tcagggcagt cttaaatttc tcttccatca catcgaaggc
    48541 ctgatcccag gtgattgggg taaattcgcc ttctttgtca tatttaccgt ttttcatacg
    48601 cagcagcggc tgcgtcaaac ggtctttacc gtacatgatt ttgggcagga aatagccctt
    48661 aatgcagttc aggccacggt taaccggtgc gtccgggtcg ccctgacagg ccaccacacg
    48721 cccctgctgc gttccgacca gaacgccgca acccgtaccg cagaaacggc acggcgcttt
    48781 atcccattta atggcttcct gctgaccaac aacggcgcgg gcaacgcccg gcacgctgag
    48841 accggcagcc gccgcagcgg ccgcaacggc gttagctttc ataaagctac gacgactgag
    48901 tttcatggtg tttcctcacc ttgctcttcc tgctggtgat aaaccagcga caccgccagc
    48961 acgccctcta cgttgcgtac tgactcaatg gtttggatca gcgtttcgct gtcttctgct
    49021 tccaccacca caatcaactg accgctcggc gcgtcgctga cagcaacttc acagccggta
    49081 aaggcgttca gttgggtgct gatgtctgaa attcgttcgc ttttggcctg cacgaccagg
    49141 ctgcaaactt gccagttagt gtgcatggag atactccgca gttatggctg ataccggaca
    49201 gctggctgca catgctccac atccgttgca aagttggcta ttaagttgcg gctggtagat
    49261 cccggagagc gtcgggcgaa agataattgc cattggctca cagctatcct gacagcggcg
    49321 gcattcaact gactgatacg ccaggcaggc ttccccgata gtgaactgca aatcccaggc
    49381 cctggtgtgg cgcggagaaa atagtgattc ggggcaagcc tgcgcgcagg cgtagcagaa
    49441 gctgcactcg ttatttttga aattaacgct cggataacca cccgcgccgc gttgcagaat
    49501 gttgttttca caagcattaa tacaggcgtc gcagcgggtg caatgggtca gaaaatgaga
    49561 ttcatcaccg gaccagggcg gacggatacc gttactggct ttgcgccagc gaccagtgag
    49621 tatgccccga cgggatgcat caatcttcac attgaccttc catcattaac gcgctctgaa
    49681 aattgagagc gaccaaataa accgcataat taataagcca tttttatagc cgctaagata
    49741 ttaaaggatg tgtcaaagat gcataccccg atcggggtaa aacctgtagc aggatcaaaa
    49801 gagtggcggg aagcgtggca aaaacgggct tttgctcaca tttcaaatgg ttataaacat
    49861 atttatatag cgat
//
LOCUS       DADTFG010000012        49817 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.012, whole genome shotgun sequence.
ACCESSION   DADTFG010000012 DADTFG010000000
VERSION     DADTFG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.012"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            125..757
                     /gene="comR"
                     /locus_tag="J1138_000740"
     CDS             125..757
                     /gene="comR"
                     /locus_tag="J1138_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBA8452580.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(819..1358)
                     /locus_tag="J1138_000741"
     CDS             complement(819..1358)
                     /locus_tag="J1138_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBA8452581.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKA"
     gene            complement(1585..2889)
                     /locus_tag="J1138_000742"
     CDS             complement(1585..2889)
                     /locus_tag="J1138_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA8452582.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3288..3830)
                     /locus_tag="J1138_000743"
     CDS             complement(3288..3830)
                     /locus_tag="J1138_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA8452583.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3853..4878)
                     /gene="nagZ"
                     /locus_tag="J1138_000744"
     CDS             complement(3853..4878)
                     /gene="nagZ"
                     /locus_tag="J1138_000744"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBA8452584.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4889..5713)
                     /gene="thiK"
                     /locus_tag="J1138_000745"
     CDS             complement(4889..5713)
                     /gene="thiK"
                     /locus_tag="J1138_000745"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBA8452585.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENIDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5694..6335)
                     /gene="lpoB"
                     /locus_tag="J1138_000746"
     CDS             complement(5694..6335)
                     /gene="lpoB"
                     /locus_tag="J1138_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBA8452586.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6349..6726)
                     /locus_tag="J1138_000747"
     CDS             complement(6349..6726)
                     /locus_tag="J1138_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBA8452587.1"
                     /translation="MRKGCFGLMSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6729..7088)
                     /gene="hinT"
                     /locus_tag="J1138_000748"
     CDS             complement(6729..7088)
                     /gene="hinT"
                     /locus_tag="J1138_000748"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBA8452588.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLTHKGL"
     gene            7423..9612
                     /gene="fhuE"
                     /locus_tag="J1138_000749"
     CDS             7423..9612
                     /gene="fhuE"
                     /locus_tag="J1138_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBA8452589.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQNDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLTPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTFKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9672..11105)
                     /gene="ptsG"
                     /locus_tag="J1138_000750"
     CDS             complement(9672..11105)
                     /gene="ptsG"
                     /locus_tag="J1138_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBA8452590.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11400..12197)
                     /locus_tag="J1138_000751"
     CDS             complement(11400..12197)
                     /locus_tag="J1138_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA8452591.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12208..13212)
                     /gene="holB"
                     /locus_tag="J1138_000752"
     CDS             complement(12208..13212)
                     /gene="holB"
                     /locus_tag="J1138_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBA8452592.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQAALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13209..13850)
                     /locus_tag="J1138_000753"
     CDS             complement(13209..13850)
                     /locus_tag="J1138_000753"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBA8452593.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13840..14862)
                     /gene="yceG"
                     /locus_tag="J1138_000754"
     CDS             complement(13840..14862)
                     /gene="yceG"
                     /locus_tag="J1138_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBA8452594.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14865..15674)
                     /gene="pabC"
                     /locus_tag="J1138_000755"
     CDS             complement(14865..15674)
                     /gene="pabC"
                     /locus_tag="J1138_000755"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBA8452595.1"
                     /translation="MFLINGHKQESLTVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVIPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15799..17040)
                     /gene="fabF"
                     /locus_tag="J1138_000756"
     CDS             complement(15799..17040)
                     /gene="fabF"
                     /locus_tag="J1138_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBA8452596.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17127..17363)
                     /gene="acpP"
                     /locus_tag="J1138_000757"
     CDS             complement(17127..17363)
                     /gene="acpP"
                     /locus_tag="J1138_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA8452597.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17574..18308)
                     /gene="fabG"
                     /locus_tag="J1138_000758"
     CDS             complement(17574..18308)
                     /gene="fabG"
                     /locus_tag="J1138_000758"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBA8452598.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18321..19250)
                     /gene="fabD"
                     /locus_tag="J1138_000759"
     CDS             complement(18321..19250)
                     /gene="fabD"
                     /locus_tag="J1138_000759"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBA8452599.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19266..20219)
                     /locus_tag="J1138_000760"
     CDS             complement(19266..20219)
                     /locus_tag="J1138_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBA8452600.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20287..21357)
                     /gene="plsX"
                     /locus_tag="J1138_000761"
     CDS             complement(20287..21357)
                     /gene="plsX"
                     /locus_tag="J1138_000761"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBA8452601.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21438..21611)
                     /gene="rpmF"
                     /locus_tag="J1138_000762"
     CDS             complement(21438..21611)
                     /gene="rpmF"
                     /locus_tag="J1138_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBA8452602.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21663..22184)
                     /gene="yceD"
                     /locus_tag="J1138_000763"
     CDS             complement(21663..22184)
                     /gene="yceD"
                     /locus_tag="J1138_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBA8452603.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22383..22967
                     /gene="yceF"
                     /locus_tag="J1138_000764"
     CDS             22383..22967
                     /gene="yceF"
                     /locus_tag="J1138_000764"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBA8452604.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23079..24038)
                     /gene="rluC"
                     /locus_tag="J1138_000765"
     CDS             complement(23079..24038)
                     /gene="rluC"
                     /locus_tag="J1138_000765"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBA8452605.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24611..27796
                     /gene="rne"
                     /locus_tag="J1138_000766"
     CDS             24611..27796
                     /gene="rne"
                     /locus_tag="J1138_000766"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBA8452606.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTVETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPKVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27991..28944)
                     /gene="flgL"
                     /locus_tag="J1138_000767"
     CDS             complement(27991..28944)
                     /gene="flgL"
                     /locus_tag="J1138_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HBA8452607.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSLAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28956..30599)
                     /gene="flgK"
                     /locus_tag="J1138_000768"
     CDS             complement(28956..30599)
                     /gene="flgK"
                     /locus_tag="J1138_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBA8452608.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSTADPSRTTVAYIDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAGAFNTQHKAGFDANGDAGKDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30665..31606)
                     /gene="flgJ"
                     /locus_tag="J1138_000769"
     CDS             complement(30665..31606)
                     /gene="flgJ"
                     /locus_tag="J1138_000769"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBA8452609.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPHNYDDSLPGNSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAELGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31606..32703)
                     /locus_tag="J1138_000770"
     CDS             complement(31606..32703)
                     /locus_tag="J1138_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBA8452610.1"
                     /translation="MIKFLSALILLLVITAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32715..33413)
                     /gene="flgH"
                     /locus_tag="J1138_000771"
     CDS             complement(32715..33413)
                     /gene="flgH"
                     /locus_tag="J1138_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBA8452611.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33466..34248)
                     /gene="flgG"
                     /locus_tag="J1138_000772"
     CDS             complement(33466..34248)
                     /gene="flgG"
                     /locus_tag="J1138_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBA8452612.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34420..35175)
                     /locus_tag="J1138_000773"
     CDS             complement(34420..35175)
                     /locus_tag="J1138_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HBA8452613.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLLADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35195..37120)
                     /gene="flgD"
                     /locus_tag="J1138_000774"
                     /pseudo
     CDS             complement(35195..37120)
                     /gene="flgD"
                     /locus_tag="J1138_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(37132..37536)
                     /gene="flgC"
                     /locus_tag="J1138_000775"
     CDS             complement(37132..37536)
                     /gene="flgC"
                     /locus_tag="J1138_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBA8452614.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37540..37956)
                     /gene="flgB"
                     /locus_tag="J1138_000776"
     CDS             complement(37540..37956)
                     /gene="flgB"
                     /locus_tag="J1138_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBA8452615.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38111..38770
                     /gene="flgA"
                     /locus_tag="J1138_000777"
     CDS             38111..38770
                     /gene="flgA"
                     /locus_tag="J1138_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBA8452616.1"
                     /translation="MLAIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            38846..39139
                     /gene="flgM"
                     /locus_tag="J1138_000778"
     CDS             38846..39139
                     /gene="flgM"
                     /locus_tag="J1138_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBA8452617.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39144..39560
                     /gene="flgN"
                     /locus_tag="J1138_000779"
     CDS             39144..39560
                     /gene="flgN"
                     /locus_tag="J1138_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBA8452618.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITGKTQQLRQLNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39600..41135)
                     /gene="murJ"
                     /locus_tag="J1138_000780"
     CDS             complement(39600..41135)
                     /gene="murJ"
                     /locus_tag="J1138_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBA8452619.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWTAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41245..42168)
                     /locus_tag="J1138_000781"
     CDS             complement(41245..42168)
                     /locus_tag="J1138_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA8452620.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGQASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42170..42817)
                     /locus_tag="J1138_000782"
     CDS             complement(42170..42817)
                     /locus_tag="J1138_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HBA8452621.1"
                     /translation="MKYQLTAMEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLASREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAADGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42828..43412)
                     /gene="rimJ"
                     /locus_tag="J1138_000783"
     CDS             complement(42828..43412)
                     /gene="rimJ"
                     /locus_tag="J1138_000783"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBA8452622.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43648..44856
                     /gene="mdtH"
                     /locus_tag="J1138_000784"
     CDS             43648..44856
                     /gene="mdtH"
                     /locus_tag="J1138_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBA8452623.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44920..45567
                     /gene="grxB"
                     /locus_tag="J1138_000785"
     CDS             44920..45567
                     /gene="grxB"
                     /locus_tag="J1138_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBA8452624.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45701..46261
                     /locus_tag="J1138_000786"
     CDS             45701..46261
                     /locus_tag="J1138_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA8452625.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46367..47413
                     /gene="pyrC"
                     /locus_tag="J1138_000787"
     CDS             46367..47413
                     /gene="pyrC"
                     /locus_tag="J1138_000787"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBA8452626.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPILERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47487..47732
                     /gene="dinI"
                     /locus_tag="J1138_000788"
     CDS             47487..47732
                     /gene="dinI"
                     /locus_tag="J1138_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBA8452627.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            48022..48276
                     /gene="bssS"
                     /locus_tag="J1138_000789"
     CDS             48022..48276
                     /gene="bssS"
                     /locus_tag="J1138_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBA8452628.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            48391..49509
                     /gene="solA"
                     /locus_tag="J1138_000790"
     CDS             48391..49509
                     /gene="solA"
                     /locus_tag="J1138_000790"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBA8452629.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPAHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49557..49670
                     /locus_tag="J1138_000791"
     CDS             49557..49670
                     /locus_tag="J1138_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HBA8452630.1"
                     /translation="MRRLVNYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        12117 a        13386 c        12318 g        11996 t
ORIGIN      
        1 atttaaaaac ctttaaattt cagttaaata taaagcgatt atttttatgg tttctggact
       61 ggtgaacgga ggtgattatt tttataataa cctttcattc acagtcacca ggtacaacgg
      121 catcatggca actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt
      181 cgacagggat gccgcgcttg ataaggccat gaaattgttc tggcagcacg gttatgaagc
      241 gacttctctt gcggacctcg ttgaagcgac cggagctaaa gcgcccacgc tatacgcgga
      301 atttaccaac aaagaggggt tattccgcgc cgtactcgac cgctatatcg atcgttttgc
      361 cgccaagcat gaagcacagc tgttttgtga agagaaaagc gtggagtctg cgctggctga
      421 ctattttgct gccatcgcca actgctttac cagcaaagac actccggctg gctgcttcat
      481 gatcaacaac tgcaccaccc tctccccgga ttcaggagat atcgccaata cgctgaaatc
      541 acgccatgcg atgcaagagc gcactttgca gcagttttta tgtcaacgac aagcgcgcgg
      601 ggaaatcccg acccactgtg acgtgacgca tctggcagaa ttccttaatt gtattattca
      661 ggggatgtcg atcagcgcac gcgaaggtgc atcgctggaa aaactgatgc agattgcccg
      721 aacgactttg cgtttatggc cggaactgct gaaataggtc gcaaaaaaag cccctcagct
      781 tgagcgaatg aggggctaaa ttgcagagta caaccttgtt atgctttgtt attcaaaatc
      841 agctgcccgt tgctatctag cgggatctgc gtacccggat cgcggtccat tcggattttg
      901 ccctgctgat cgccaatctt ataggtcaca tcataaccga gcattttttc tgacttgtca
      961 tacaccgttt tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc
     1021 tggatctggt tacctgcata tccacccccc agcgccccca caacagtggc gacatcttta
     1081 ccgcgaccac caccaaactg atgcccaatc acgccgccag caacagcgcc gagcaccgac
     1141 ccggtaatgc gattttcatc ctgcaccggt cgacgatggg tcactgtaac gttgcgacac
     1201 tcctgacgcg gtgttttaac cgtttccttg attggggttg cagaaacaac ctgagcgtat
     1261 tgcgggccac gttcaaacac gttcagactg gccactgccg ctacgcccag cgcagctgcg
     1321 acaccaatcc cgatacccgc caacattgac ttattcacgg gaatacctcc ttctttgctg
     1381 ttatgccaat catgcaagcg aaggagaaaa gcagctatca gacagaagat gaaaagcgcg
     1441 ggaatggctg gaaatactta gggattcgga ggagtctgaa ggaatgcctg atgcgacgct
     1501 taacgcgtct tatcaggcct accgctcgta ccatttgtcc agggcggata aggcgtttac
     1561 gccgcatccg ccagtacaag ccgattaatg caacttcaaa cgtggacgga taacgcggtt
     1621 aatactcccc accagcatca ttaatccggt tttaaagtaa ccatgcagcg caatctggtg
     1681 cattcggtac agcgagatgt atacaaagcg cgcaattcgt ccttcaatca tcattgagcc
     1741 gcgtgtcagg ttacccatca ggctaccgac ggtggaaaag ttcgacagcg ataccagcga
     1801 accgtgatct ttatactgat aatttttcag cggcttaccg ttcatctgcg ccagaatgtt
     1861 gttcattgcg caggttgcca tctgatgtgc ggcctgagcg cgcggcggaa caaagccccc
     1921 ttccggacgc gggcatgacg cgcagtcgcc aatagcgtat atgtctggat cgcgagtggt
     1981 ttgcagcgtc ggttccacca ccagctggtt gatacggttg gtttcaagac caccgatatc
     2041 tttcaggaag tctggcgctt tgatcccggc tgcccacacc atcagatcag cctcaatata
     2101 ttcgccatct ttagtgtgca ggccgccttc atcagcactg gtgaccatgg tttgcgtcag
     2161 cacgcgaacg cctagtttcg ttaactcact atgggccgca gcagagatac gtggcggcaa
     2221 tgcaggcaaa atacgttcgc ccgcttctac cagcgttacg ttcagggctt cgttggtcag
     2281 acctttataa ccgtaactgt gcagttgctt gaccgcgttg tgcaattcag cggagagttc
     2341 tacacccgtc gcgccgccgc cgacaatcgc aatgtttact ttgccattcg cgcccaggtt
     2401 ggcggagtat ttcaggaaca gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg
     2461 gttatcgagg aaaatgcagt tctctttgac acctggtgta ttgaaatcgt tagaggtgct
     2521 acccagcgcc attaccaggg tgtcataggc gattttacgt tccggaacca gcagttcacc
     2581 tttctcgtcg cgcagttctg caatagtgat tgttttcgct tcacgatcaa tatcaattac
     2641 ggaacccagc tggaactgga aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc
     2701 gacgccttca tcaagcgagc cagtcgccac ttcgtgcagc agcggtttcc acaggtggct
     2761 gtggttacga tcgaccagcg taattttggc ttttttcttg cgtcccagct tatgccccag
     2821 ctgtgttgcc atttccagcc caccagcacc gccgccgaca atcacaattt ttttcaatgg
     2881 cgtagtcaac gtgaccccct taaatttatt aaccaattgt taattaatgg ttattaacat
     2941 agctcttaat taacaacagg ttacagcaat gaaaatgttc ttcgctattg agaataacat
     3001 gaatggtgca ttggtcatac caaaattgat gttaatcaag ttttgttgct gaaaagatag
     3061 gcgactttac cgggtgagat caaaaaacag agtttgttgt ttattatttg agctgatttg
     3121 tgagaatgat atagagtgag gtgtgtatgg agggcgttcg gtaggcctga taagaggcgc
     3181 atcaggcatt gatgctcgac tgtcggatgc ggcggaacgc cttatccaac ccacagaatc
     3241 cggagcgcat caggtaccgg caaggttacc tgatgcgacg agggcattta cccgagggtt
     3301 ttgaacgctt taatgcgctg taaatgcggg gagatattct tgaatttgtg cgtctgctct
     3361 tcgtcccaga caatctcgta ataatgatgc aactcttcag atgtccgctg gctgttaagc
     3421 gcttcatcat tacgcgacaa aatcaccagg caacgatcgc gattcttctc acggaagttg
     3481 gtcacacact tagtggcaat atcggcatac tcttccgggc gatcaatctt cccttccatg
     3541 ttctcataag ggaacaaatt ggggttgaag atcacctggc ggatatcaca gagaaaacca
     3601 atccgctcag cccaataacc gcccagacca acaccacaaa tgagcgggcg ctcgtcgacg
     3661 ttcagttgca acattttgtc cacttctttg agcaaatgct gcatatcatg tttcggatgc
     3721 cgcgtactgt agcttattag ccgtacatcc gggtcaataa actgcaattg taagactttc
     3781 tcgtggttac ccggactgtt agagtcaaaa ccgtgcaaat agataatcat cgcttcctca
     3841 cataagccag ggttaatgac ctgccttctc ttcctgccag cgttcatgta actgattcag
     3901 acgggtgctg atcgctttcc aacgagccga gtccatcagt tcctgtcgcg aaaatgaacc
     3961 tttatgatac aaacgtgtaa cacgttctgc cttgatcggt gacagattat ctaacacgct
     4021 gacggcccct ttacgattat tgcagaccag gatcatatcg caacctgcat ccagtgatgc
     4081 ctgtccacgc tcggcataac tgcccataat cgctgcgcct tccatcgata aatcgtcaga
     4141 gaaaatcacg ccgtcaaaac ccaattcctg acgcaaaacg gttttcagcc agtagggaga
     4201 accgctcgcc ggacgcggat caacatcact gtagatcaca tgcgcaggca taatggcgtc
     4261 gagtttattt tcgcggatta aggaactgaa gaccgacata tctttagcgc gaatctccgc
     4321 ttgcggacgt gggtcgcacg gtgtttcttt gtgtgagtct gccgttactg caccgtgacc
     4381 tgggaagtgt ttcccggtag ttttcattcc cgcttcatgc ataccatcaa taaaacggct
     4441 ggcaattgcc agggcttttt gtggatcggc atgataagaa cgctcgccaa tcgccgcgct
     4501 gatatgcccg acgtccagca ctggcgcaaa gctgatatca atatccatag caatcatttc
     4561 actggccatc aaccaacctg cctcttgcgc cagttttccg ccttcttcca ttcccaacag
     4621 cgcagcgaat gattgtgccg ctggcaagcg ggtaaaacct tcacgaaaac gctgcacgcg
     4681 tccaccttcc tgatctaccg ccaccaccag atgattgcgc gatgctgcgc ggatctggcg
     4741 caccagttca cgtaactggg caggatcatg atagtttcgc gtaaagagaa tcagccctcc
     4801 caccagcgga tgcgccagta tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc
     4861 caacattact ggacccacac tgctctcctt attgttttat taatagctgc cgccacgtgt
     4921 catcggccag cctgataaat tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc
     4981 ctgctttgag catcagcagc cagggaaacc atcgcctgac ctgacgccat aattgcgccg
     5041 gataaatctt cgcgcgagtg gcatagtcat tgaccaattg ccgatgctgg tcaatatttt
     5101 ccacccacac cgccgccagt tccagcgcga tatcaccatc tccggcatac tcccagtcga
     5161 tgagttttaa ccctgacgcg ctatgcacta aatttccggc gtggacatcc atatgcaatg
     5221 gacttaagcg taaaggccgt ggttcccgcg ctttgcgcag acgttttaac attcgcagcc
     5281 aacccactgt ccgccgcgcc ggatcgcttt gctgccagta cagttccagt aacggcaaca
     5341 gcgttattcg ccagccaaaa cgcggttgtt gatgtagata atacagcaag cctgccagtt
     5401 cgttggtatc cggcaaatac gtttttacct cgccgggcag atagtcgacc accatccagt
     5461 cacggagata taaatgcggc ttcggtgcaa tgcatgcggg tagttgtgat aaagcccgat
     5521 actggcgcaa gaacgcggac tgcggcgcat caggatcgtg cggctgacgc acaacaaaac
     5581 gctggccctg atgttcaatg agaaaactcc cgccactaag cccactatta aacgtcgtga
     5641 cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc gcgcgtgagg ggattattgc
     5701 tgcgaaacgg cacctttacc tgaccagata atttcgcccg tctgcaccag catcagctgc
     5761 atttgtaggg tcggagcgtt aacgttgcca gaggcgctgg agtacagcac gtaatgagcg
     5821 ccaacattgc gggcaatgcc tatggcttta ctacgggtgc ctaaactgtc ctgcggcgat
     5881 aaacctaatt gttgcttagc cattgacagc tgctgggcgg aaaccagggt aaatttaccg
     5941 ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg cggcattcaa cgaaccgtta
     6001 gtacggttgt taacgctatc aaccagcagg acgctacccg cagtcacgcc gtcagcccca
     6061 agcatcttac tgaccatcgg ctgcattgcg ccattccagt cataatgacg aatatgcggc
     6121 gcaggcggtg cagtttgatc ttcgtgctca attgggcctg gctgctgcgg tatcgtcggc
     6181 accgatggca ctgtggggac aggctgttgt ggctcagcag gttgttccgg cgctggtttc
     6241 acttcttcta ccggtgcagg ttcacgttgc cccacacacc cggcaagaaa catcgccagc
     6301 gcggtaatca aggcgtagcg actcattttt gtcatcaaga ttcacccctt acaaatatag
     6361 ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg tgaccgccga
     6421 atgtgcggga atggtaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc
     6481 gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaagcgt
     6541 tgaggatgct gaaggttgaa tatcagatgt cgaaaggacg ggcttttctg cactgatgcc
     6601 cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg
     6661 atgtgaacga cagcccacca gcactaacaa cgccagagac atcagcccaa agcatccttt
     6721 tctcatcgtt acagaccttt atgcgtcagc attggtccca gcggacggcc acccaacaaa
     6781 tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtgttcat gatcagacga
     6841 tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt
     6901 cccagcgcct gctcatgctc agctgacacg tcgttcacag tcggaatgag gatattcgga
     6961 atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca
     7021 tcctggtaga cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct
     7081 tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca
     7141 cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaaccttaa ctcacgattt
     7201 cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt tctgcctgcg
     7261 aatgcgctga aaagacattc tctgtttggt ttacactcca aatatgaatg cgtatatttc
     7321 tcatttgcat ttacacacaa aaattattcg cacactaaat ataaccgctc atcaccctgc
     7381 ttcggcatga gcctgacgcc aacataaacc aagagatttc aaatgctttc gacacaattt
     7441 aacagggata atcaacatca agccatcatc aaaccgtcac tccttgccgg ttgcatagca
     7501 ctggcactat taccttccgc cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt
     7561 gagggttcag ccacagcccc agatgatggc gaaaatgatt acaacgtaac gtctacctct
     7621 gcgggtacaa aaatgcagat gactcaacgt gatattcctc agtcggtcac tattgttagc
     7681 cagcagcgga tggaagatca gcagttacaa acgctgggcg aagtgatgga aaacacgctg
     7741 gggatcagca aaagtcaggc ggattccgat cgtgctcttt attattcccg cggattccag
     7801 atcgataact atatggttga tggtatcccc acctattttg aatcgcgctg gaatctgggc
     7861 gacgcacttt ctgatatggc actgtttgaa cgcgtagaag tagtgcgtgg cgcgacaggc
     7921 ctcatgaccg ggacgggtaa tccatctgcg gcaattaata tggttcgaaa acacgcgacc
     7981 agtcgtgaat ttaaaggcga tgtctcggcg gaatacggta gctggaacaa agaacggtat
     8041 gtggcggatt tacaaagccc actcaccgaa gacggtaaaa tccgcgcacg aattgtcggc
     8101 ggctaccaga ataacgactc atggctggac cgctacaaca gtgaaaagac cttcttctcc
     8161 ggcattgtcg atgctgattt aggcgatctt acgatgcttt cagccggtta cgaatatcag
     8221 cgtattgatg ttaatagccc aacctggggt ggtttaccgc gctggaatac tgatggcagc
     8281 agcaacagtt acgatcgcgc acgcagtacc gcacctgact gggcgtacaa cgataaagag
     8341 atcaacaagg tctttatgac cctgaagcag cggtttgctg atacctggca agcgacgctg
     8401 aatgccaccc actctgaagt cgaatttgac agcaaaatga tgtatgtcga tgcctacgta
     8461 aacaaagcgg atggtatgct ggttgggcca tacagcaatt atggacctgg ctttgattat
     8521 gtcggcggca ccggttggaa cagcggcaaa cgtaaagttg atgcgctgga tctgttcgct
     8581 gacggtagtt atgaattgtt tggtcgtcag cacaatttaa tgtttggtgg cagttacagc
     8641 aaacaaaaca atcgttactt cagttcatgg gccaacattt tcccggatga aattggcagt
     8701 ttctacaact ttaatggcaa tttcccacaa accgactggt caccacagag cctggcgcag
     8761 aacgatacca cacatatgaa atcgttatat gctgccactc gtgtcaccct tgccgatccg
     8821 ctgcatctga tcctcggcgc acgttatacc aactggcgag ttgatacgct gacttacagc
     8881 atggagaaaa accacaccac gccttatgct ggactggtgt ttgacatcaa tgacaactgg
     8941 tcgacctacg ccagctatac ctctattttc cagccgcaaa atgatcgtga cagttcaggc
     9001 aaatatctga ctccaatcac cggtaacaac tacgagctag gtctgaaatc ggactggatg
     9061 aatagccgtc tgaccaccac gttagccatc ttccgtattg agcaggataa tgtcgctcag
     9121 tccaccggta cacctatccc cggcagcaac ggcgaaaccg cctataaagc ggtggatggg
     9181 acagtcagta aaggtgtgga atttgaactc aacggcgcaa ttaccgacaa ctggcagttg
     9241 acgtttggcg caacgcgcta tattgccgag gataacgaag gaaacgccgt taatcctaat
     9301 ctgccacgca ccacgtttaa aatgttcacc agctatcggt tgcctgtcat gccagagttg
     9361 acggtcggcg gtggtgttaa ctggcaaaat cgcgtgtata ccgacaccgt gacgccgtat
     9421 ggcaccttcc gcgccgagca aggtagctac gcgctggtgg atctcttcac ccgctaccag
     9481 gtgacgaaaa acttctcgtt acaggggaat gtcaataacc tgttcgacaa aacctacgat
     9541 accaacgtgg aaggttctat cgtctacggc gcaccgcgta atttcagcat taccggcacg
     9601 tatcaattct gaataacacc tgtaaaaaag gcagccatct ggctgcctta gtctccccaa
     9661 cgtcttacgg attagtggtt acggatgtac tcatccatct cggttttcag gttatcggat
     9721 ttagtaccga aaatcgcctg aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt
     9781 ttcttcaggc cagcctgatc cactttagac acatcagcaa cgctgacgcg cagacgggta
     9841 atacatgcgt cgaggttagt aatgttttct ttaccaccaa atgcagcaac cagagccggt
     9901 gccatttcgc tggtacctgt cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt
     9961 ttcagatcca gtgctttaat cagcacgcgg aagatggtgt agtaaacaat ggcataaccg
    10021 ataccgacga tcgggaacag ccacagtttg ctgctgttac cagacagaac gatgaagtcg
    10081 atcagaccgt gcgagaacga cgtaccgtca cgcatcccca gaagaataca gattgggaat
    10141 gccaggcctg ccagaatcgc gtggatgatg tacaggatcg gcgcaacgaa catgaaggag
    10201 aactcgatcg gctcggtgat accggtcagg aacgaggtca gcgccgcgga gatcataata
    10261 ccgcccactt tcgcgcggtt ttctggttta gcagagtgcc agatagcaat tgcggcagct
    10321 ggcagaccgt acattttgaa caggaagcca ccagacagtt tacccgcagt cgggtcaccc
    10381 gccatataac gcggaatgtc gccgtggaaa acctgacctg ctgcgttggt gtattcacca
    10441 atctgcatct ggaaaggtac gttccagatg tggtgcagac caaacggtac caggcaacgt
    10501 tcgatgaaac cgtaaatgcc aaacgcaact accgggttct ggtaagcagc ccactgagag
    10561 aaggtctgga ttgcagaacc aatcggcggc caaatgaagg acagcacaac gccagtaaag
    10621 atggcagcca ggcctgaaat gatcggcaca aagcgtttac cggcaaagaa gccaagatac
    10681 tcaggcagct taatacggta gaaacggtta aacatgtacg ctgcgatcgc accggagata
    10741 atccccccga gtacgccagt atccgccagg tgtttagagg cgatttcttc agcaggtaaa
    10801 tgcagtacca gtggcgcaac cacggccatg gttttaacca tgatgccata ggcaacaact
    10861 gcggccagcg cggatacgcc atcgttattg gtaaagccga gggcgacacc gatcgcaaaa
    10921 atcagtggca tgtttgcaaa gacggaaccg cctgcttctg ccataacatg cgatacaacg
    10981 gcgggcagcc agctgaaatt cgcggaaccg acgcccagca gaatacctgc gataggcagt
    11041 acggataccg gcagcatcag cgatttaccg accttttgca ggttagcaaa tgcattctta
    11101 aacataattg agagtgctcc tgagtatggg tgcttttttt acgttctcac gcgtggcaag
    11161 gggggagtgc ctcgccgtgt acagggcatc taagcgccct ttatttatta cacagagtaa
    11221 aataattcag tgccgatttg tttgacggct atcacgtttc aacttcagat cattggagaa
    11281 gttgcacatt ttgacaaaac atatgtcaca atatttcaaa cgtggttatt caccgcccga
    11341 ttttcggcgg tgaactttac tcgttttagc cattaattac gagctttaaa aaaactcatt
    11401 caacggatgg attgaaggcg ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg
    11461 gttacctgcg ccagttcttc aacggcaaca cctttcaaca cagccatgta ttctgcaacg
    11521 tcacgaacca tcgcaggttg attctctttt cctcgatgcg gtactggcgc aaggtaaggt
    11581 gagtcagttt ccaccagtaa ccgatccagg gggacataac gcgcagcatc gcgcagttgc
    11641 tccgcattac ggaaggtcac aatgccggaa aaggagatgt aaaatccgag atccagtaat
    11701 ttgcccgccg tttctctgtc ctctgtaaaa cagtgtagta cgccaccgca atccgtcact
    11761 ttttcttcgc gcagaattgc cagcgtatcg gcgcgggcgt cacgggtgtg gacgataacc
    11821 ggcttgttca gttcacgacc aatctggata tgatggatga aggactcttg ctgacgtact
    11881 ttagtttccg gcgtgtaata ataatccagc ccggtttcac ccagcgctac aacgccctct
    11941 tctgccgcca gacgacgtaa atcttctaca tcgtagggat cattctggtt gagtggatgc
    12001 acgccacaag aaaatacgac gttgtcgcgt tcgcctacca gatcccgcat gtgtaaataa
    12061 cccggtaatg ttgtggcgac tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc
    12121 agaacgtcat ccacgtcctt atgcaaagat tcataatcca gaccatcgag atggcagtgt
    12181 gagtcgacta aaaacatgat gtctctctta aagatgagga accggtagca caacgcccgg
    12241 ttgcaggtaa tgctcaatac gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc
    12301 tgtgacagac attaactgtt cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg
    12361 cgagggagaa agatggtttg ccagttcgac gaccaggccc ggcacatcaa cattggtcac
    12421 ctgcgcagcg ccatgatggc gttttagcgc atccatcagc aacgttgcca gccagtgtaa
    12481 acgtgccgga gcttgttcat gattaagggc cgctagcagc gaataccagt cgcccgattg
    12541 cacgctatat gccagcgcct gacacaatgt ttcacgagcc tgccagttat ctccctgaaa
    12601 caacgccagt gccgccccag gcgaaccggc gcttaagcgc aatgcggcaa gtagtgcagc
    12661 ctgtgacatt gtcacttcgc gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc
    12721 aaggtaatgt aaccgacaac gactacgtaa tgttgccagt aaacgttctg gctcgcgggt
    12781 agcaaggaaa aaccacgtct ctgctggtgg ctcttcaagc gttttcagca atgcgttagc
    12841 cgcggcgtcg gttagtaagg cagcatcggt tacccagaca actttcgcac cacctaagcg
    12901 tgcgtgctca ttcagctttt cggtgacctc acgtaccgca tcaatgccca gcgcattttt
    12961 ccctttttcg ggagccaggg tgtagtaatc gggatgtgtg ccagcctgca tcaactgaca
    13021 tccacgacag tgaccgcaac ttttgtggcc ctgcggttgt tggcagagta aataacggct
    13081 cagggcgtag attaaagcat catcgcccat gcccggtaac gcctgaatga gtagcgcatg
    13141 gtgacctctt ccggcctgat agctggctac cagtttttcg aaatcaggtc gtaaccatgg
    13201 ataccatctc atgcgtccaa ctccttcacc cagttggtca cggtagtgcg gattgcatcc
    13261 atcacggcct ccagcggctg ggtggcatca atggtatgaa tgcttttatc ttgtgctgcc
    13321 agttccagat agcgggcgcg ggtgcgatta aagaaatcga atgactcttg ctcaatacga
    13381 tccagctcac cacgggcgcg agcacgtttt aagccgacct ctggggtgac atcaagataa
    13441 agcgttaagt cagggcgaaa atccccgaga acagcatcac gcagtgttgc cagcatatgt
    13501 tggtcaatac cacgtcctcc accctgatag gcttgagtgg agagatcgtg gcgatcgcca
    13561 atcacccagg tgccgttagc cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc
    13621 gcggcataaa acatcagaac ttcggcttta tcggtaatga cttcatcgcc taccgatttg
    13681 atatccagca ccaggcttct taacttttcg gcaagttgcg taccgccagg ttcccgagtg
    13741 aaaaccatgt cgcggatacc cagttgctca agcgtctcaa ccaccacgtt acgcgcggta
    13801 gttttgcctg cgccttccag cccctcaatg acgatatact tactgcgcat ttttttcctt
    13861 aagcactttc agataatcct gcacagactt gttatgactg gcaagattgg tattaaacgt
    13921 gtgaccacct ttaccatcgg ccacaaaata gagatacggc gtttttgccg gatgcgcagc
    13981 agccttcagc gaatccgccc ccggcgtcgc tatcgcacct ggcggcagac cggtaatggt
    14041 ataggtgtta tacgctgtcg gcgtttccag gtctgcacga gaaagtttgc cattataacg
    14101 ctctcccatc ccgtaaatca cggtcgggtc ggtctgcagg cgcataccaa tgcgtaaacg
    14161 gttgataaat actgaggcaa cctgatcgcg ttcactggca acggcggttt ctttttcgat
    14221 aattgatgcc atcgtcacca gctggttttt atctttatag ggcagaccgt ccgcacgccc
    14281 ttcccaggcg ctatcgaccg ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac
    14341 atcggtggta ttggcggtat acatccaggt gtctggccag aaccaacctt caatccactc
    14401 tgggttttcc agttcaagtg cctgcgctac ggtggcgtac ttatcgtcgc tcagcgtatg
    14461 cttgatatac ggggcctcac gcaattgctt gaggtaatcg ctcagacgca tcccttctac
    14521 cagtcgcaga gggaactgtg cttctttacc gctttccagc aatttcagca tctcgcgcac
    14581 ggtcatctgc ggtgtaaagc ggtaagtccc ggctttaaag tgagaaagat ccggttcgat
    14641 acgcagcagc cattggaaaa cccgtgggcg attgatgatc ttatcggcat aaagctgttc
    14701 accgagcgcc agacgtccgg tccctggctt cagggtaaat atcgtctctt ctttgataag
    14761 caatttgctg tcggcaagat ggcgaacctt ccagacgccc acaccagcgg cgatacccag
    14821 taccaccagc aataacaaga taatcaataa cacttttttc atgactaatt cgggcgctca
    14881 caaagtgggg ctaaatattc atataacgtt gctgacgaaa aggagacatc gccacaggca
    14941 cgtacgggta tcactggcat taacgcatta caaataacca tctcatctgc ctgcaacgcc
    15001 tcctccagcg aggcttgcac ttcgacaagc tgataagagg attgtgccag caaacggata
    15061 cagaattgtc gcataatgcc gttaacacct gcctgatcca gtcgcggcgt ataaactacg
    15121 ttgcccttcc gccagaacaa attagccgca cagcattccg taacccaccc ttcgctgtca
    15181 aggaccagcg cctcatcagc gtttgtctgc tcaagatgag agcggatcaa tacttgctca
    15241 agccgattaa gatgtttaat acctgcaaga tgaggattgc gccccagccg caccgggctt
    15301 agcgccaacg tcatcccttc gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg
    15361 gagagaatcc gcgttgccgg tccactgttc aatgtgctgt accctcgccc gccactaccg
    15421 cgactgatca cgactttcag cacaccattc tgctgttctg ctgccagcgt tttcatctct
    15481 tgttcaagct gaggccagaa gtcacaggaa atcatcaacc gctgacaagc atcctgtagt
    15541 cgctggatat gcgccgataa caaactgact ttaccgtcga taactctggc ggtggtaaaa
    15601 caaccatcac caaactgcgt tgcccgatcg cttactgtca gcgattcctg cttatgaccg
    15661 ttaattaaga acataccggc tcctctcctt atcagagaat tgttagtgtg gcagcatgtt
    15721 cactacggaa caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg accttttata
    15781 agggtggaaa atgacaactt agatcttttt aaagatcaaa gaaccattag tgccaccgaa
    15841 gccgaaggag ttacacagag tgtattccat tccgctaacc tgacgcgctt cgtgcggtac
    15901 gaaatccaga tcgcaacctt cgtccgggtt atccaggttg atggttggcg gaacagcctg
    15961 atcgcgcagc gccaggatgg agtagataga ttctactgca cccgccgcac ctaacaggtg
    16021 accggtcata gatttcgtgg agcttaccaa cacgcggctt gcagcttcac cgaagatggt
    16081 tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac
    16141 gtagccaatc tgacttgctt caatgcctgc atcacgcaga gcatttgcca tcgccagagc
    16201 tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc tcataccaaa
    16261 gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag
    16321 taccagcata ccggcaccat cgcccagtac gaaaccatca cgctctttat cccacgggcg
    16381 gctcgccgct tgcgggttat cattgcgggt agataatgca cgtgccgcgc caaaaccacc
    16441 aacgcccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt cagcatcgcc
    16501 atacgcgata atacgcgcag catggccaat gttgtgcacg ccggaagtac aggcggtcgc
    16561 gatagagatg cttgggccac gcaggccata catgatagtc agatgacctg ccaccatgtt
    16621 cacaatcgtt gacggaacga agaatgggct gatcttacgt ggaccaccgt tcatcagaga
    16681 tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg gagccaattg cggcaccaat
    16741 gcgggttgcg ttctcttccg ttatttcaag gccagaatcc tgcatggcct gaacgccagc
    16801 gacaattcca tattgaatga aggcatccat cttgcgctgt tctttgcgcg agataatgtc
    16861 ctcacagtta aaatccttta ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa
    16921 atggtcgatt aggctgatgc cactctgacc ggcaagcaga gctttccagg tagactctac
    16981 ggtattgccg acaggagaca acatgcccag tccggtcaca actacacgac gcttagacac
    17041 gtttgtcctc cagggaggga aaaaatgatt ctagtgggac aaaagataaa actcaggcgg
    17101 tcgaacgacc gcctggagat gttcacttac gcctggtggc cgttgatgta atcaatggca
    17161 gcctgaacgg tggtgatttt ctcagcttct tcgtccggaa tctcagtatc aaactcttct
    17221 tccagagcca ttaccagctc aacggtgtca agagaatccg cgcccaggtc ttcaacgaaa
    17281 gaagcattgt tggtaacttc ttcctgctta acgcccagct gttcgccgat aattttctta
    17341 acgcgttctt cgatagtgct catactctta aatttcctat caaaactcgc tttcgcgatg
    17401 gttttcgtag tgtataaaat gttgaaaaat ttgcaactaa atcccggcag gtcttaccac
    17461 gattttacgt tattttgagg ccttaccccc caataacgca aatcattttg cactaattat
    17521 tactcataac cacgcagact gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc
    17581 atgtacatcc cgccgttcac atgcaaagtt tcacccgtga tgtaagctgc ttcgtcggat
    17641 gccaggaatg caaccgcgtt ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc
    17701 tgcgccagga tacccgcacg ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata
    17761 aagcccggag caacaacgtt tacagtaata ccgcgtgacg caacttcgcg cgccagtgat
    17821 ttactgaagc cgatcaagcc cgctttcgcc gcagcgtagt tggcctgacc gccatttccc
    17881 atggtaccaa ccacagaacc gatagtgata atacgaccat gacgcttttt catcatagcg
    17941 cgcattaccg cttttgacag acggaaaaca gatgaaaggt tggtttcgat aatatcgttc
    18001 cactcttcat ctttcattcg cattaacagg ttatcacgag tgataccggc attattgacc
    18061 aggatatcca cttcaccaaa ttctgcgcga attttttcca gaacagattc gatagatgcc
    18121 gggtcggtca cattcaacat cagacctttg ccgttggcac ctaaataatc actgatcgcc
    18181 tgagcgccat tttcactggt cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt
    18241 tcagcaattg cgcggccaat tccgcggctt gcaccggtta ccagtgcgat ttttccttca
    18301 aaattcatga ttttcctctt ttaaagctcg agcgccgctg ccatcgctga aggttcgttc
    18361 agcgccgagg cggtcagagt gtcgacaatg cgtttcgtca ggccagtaag cactttgccc
    18421 gggccgactt catagagatg ttctacgcct tgcgctgcca tgtactcaac agacttcgtc
    18481 cactgaaccg ggttatacaa ctgacgtacc agtgcgtcac ggatggcatc accattggtt
    18541 tcgcatttca catcaacgtt attcacaaca ggaactgttg gtgcgttaaa ggtgattttc
    18601 gctaattcta ctgccagttt gtcggctgct ggtttcatca gcgcacagtg agacggtacg
    18661 ctcactggta acggcagagc gcgttttgcg cccgcagctt tacaggcagc gccagcacgc
    18721 tcaactgctt ctttatgacc ggcaataacc acctgtcccg gagagttaaa gtttaccgga
    18781 gaaacgacct gaccttctgc agcttcttca cacgctttcg caatagacgc atcatccaga
    18841 ccgatgattg ccgccatagc gcccgtgcct tccggtacgg cttcttgcat gaacttgccg
    18901 cgcatctcaa ccaaacgcac cgcatcagcg aaatcaatta caccagcgca aaccagcgcg
    18961 gagtattccc ccaggctgtg accggccatc attgccggtg ctttaccgcc ctgctgctgc
    19021 catacgcgat acagcgcaac agatgcagtc aacagcgctg gctgagtttg ccaggtttta
    19081 ttcagttctt cagctggccc ctgctgggtc agcgcccaca gatcgtagcc cagcgccgca
    19141 gaagcttctg caaacgtttc ttcgacaatt ggatagctcg ccgccatatc agccagcatt
    19201 ccaacggttt gagaaccctg tccagggaac acaaatgcaa attgcgtcat gttttaatcc
    19261 ttatcctaga aacgaaccag cgcggagccc caggtgaatc caccgccaaa ggcttcaagc
    19321 agaaccaact gtcccggctt aatgcgcccg tcacgtacag cttcatccag cgcgcacggg
    19381 acagaggccg cagaggtatt accgtggcga tccagcgtca ccacgacatt gtccatcgac
    19441 atgccgagtt ttttcgccgt tgcactgata atacgcaggt tagcctgatg cggaaccagc
    19501 cagtccagtt gagaacggtc aagattattc gccgccagcg tctcatcaac gatgtgcgcc
    19561 agttccgtta ccgcaacctt gaagacttcg ttgcccgcca tcgtcagatg aattgaattc
    19621 tctggattca cacggtcagc attaggcagc gtcagcaatt caccatagct accgtcggca
    19681 tgcagatggg tggagatgat tcccggctct tcagaggcag ccagcaccgc agcgcccgcg
    19741 ccatcgccaa aaataataat agtcccacga tctgttggat cgcaggtgcg cgccagtaca
    19801 tcggaaccga cgaccagagc atacttcacc gccccagatt tcacgtattg atcggctacg
    19861 cttaatgcat aggtgaaacc tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta
    19921 atgcccaaca tgctttgaat ctgacaagct gcgctcggga aagcgtgcgt cgcagaagtc
    19981 gttgccacaa cgatcaagcc aatctggtct ttctcaatac ctgccatctc aattgcgcgt
    20041 gtcgccgctt caaagcccat ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg
    20101 cggataccgg tacgagtgac aatccactcg tcagaggtat ccaccatttt ttccaaatcg
    20161 gcgtttgtcc gcacttgttc gggcagatag ctgccagtac caataatctt cgtatacatg
    20221 tacgctcagt cacttttcgg ttatataccg tcacttgcaa actgcgagtt cgctggcagc
    20281 gtcctgctac cgcagagttc cgcttttgcc accgtccagc agctcaaaac cagctgggta
    20341 tacagattcc aggcgagcgg caattcgctg aggaacttgt cgctgcaccg cctgcactgc
    20401 ctgttcaatc gcgaccgcaa aagctcgctg attggctgca ccatgacttt ttatcaccgt
    20461 gccgcgcaat cctaacagac aggcgccgtt atactggtcg gggttgaggt gactgaatcg
    20521 cctcgtcagg ctcttttgta gccaacgctt taataacagt agccaccacg accgtttttt
    20581 cccttcaccc tgagatttca gcagagaaag gaacatcctg acaacacctt ccatcgtctt
    20641 taatgtgaca tttcctgtaa agccgtcaca aaccagcaca tctgtcttgc cagttaacaa
    20701 ctcattggct tcaagatagc cgatataatt gatagaaggg attgttttaa gcaccgctga
    20761 ggcatcccga atactgtcga gaccctttac ttcttcttca ccaatattga gcaacgccac
    20821 gcgaggatta ggaatttcca ccacctcttc agccagaact gagcccataa tggcaaattg
    20881 caccaacatt gtactgtcac aatcgacgtt ggcccctaaa tcgaggacca ccgttttgcc
    20941 cttttgctga tgcggtaata ccgtcaccag cgccggacgc tcaatcccct ccaggggctt
    21001 gagtaataat tttgccagcc ccattagcgc cccggtatta ccggcactga cacaggcttg
    21061 cgctcgacct tctttcacca gctccagggc cacgcgcatt gaactcccac gactggcgcg
    21121 gatagcttgc gaaggccggg catcactggc gataactgac tgcgcaggaa taatctgcag
    21181 acgcgaacgt tgttcaaagt cagctttagc aagtaatggc gtgatggcgt cgggattgcc
    21241 gactaaaaga agagtgagtt gcgaattaga attcagtgcc tgcaatgctg caggcactgt
    21301 cacggaaggg ccaaaatccc ctcccatgac atctaacgcc agggttagac gtgtcaaggt
    21361 atcgtcgccg cccggtttgg tctttcccca gttgcctggg gaaaatcctc actaagcttc
    21421 atcacgcaga cgcgtgatta cttagcgatg accttgcggc cgcggtagta accgtcggca
    21481 gtgatgtggt gacgcaggtg tttttcacca gaagttttgt ctacagacag gctggtgact
    21541 gcggtcagcg cgtcatggga acgacgcatg ccacgtttgg aacgggttgg tttattctgt
    21601 tgtacggcca tggaccttac tcctcaatta cggtttatcc ccgatccaac ggggagcacc
    21661 aattacttac gctttaagct ggctaatacg gcaaatgggt ttggcttttg cgcttcttca
    21721 ggcagttcac caaagaccat gtccgcttcg gacacttcac agtgttcaga atcatgcacc
    21781 ggaactaccg gcaaggcgag gatgatttca tcttcaacca ttgcaagcag atcgatttca
    21841 ccgaattcgt taacctcaat cggttcatac gcttccggca gtgcttcagc ctgttcgtct
    21901 gaacgcacag gactaaaaca atacgttgtg tagacctgat gagtaaacgg cttcccgcaa
    21961 cgctgacact cgagcgttac cgtcaccttc gcatcgccgt ttaacactgc gagacgttgg
    22021 ttatcgatag cgaacgacat ggagcattcc acatcactgt ccacactgac tacggattcg
    22081 gcgacgcgct caacctgatc aggagtatag ataccctggt aatcaaggcg tttttgagcc
    22141 gtacgaaccg gatcgagagt caggggtaat tttacctttt gcatagggcg cgcatattaa
    22201 ctttgtaacg tcatagagtc aaagaaaaag gcagcctgtg gttgcctttt gccaataatt
    22261 cgcacacatt gcgggttact gctttatttt cgttcagtgc tgataacgaa catcaacctg
    22321 caaaacgaag cgcatagttt aaaatggcta tcatcaatac gctatatctg atggaaaaaa
    22381 atatgcctaa acttatttta gcctccacct cgccctggcg tcgcgctctt ctggaaaaac
    22441 tgcaaatctc ttttgaatgt gcagcacccg aggtcgacga aactccacgc agcgacgaat
    22501 caccgcgaca gttggtgctt cgactggcac aagaaaaagc gcaatctctg gcgtcacgtt
    22561 atccggatca tttaattatt ggttcagacc aggtatgtgt tcttgatggc gaaatcaccg
    22621 gtaaaccgtt aacggaagaa aatgcccgtc tgcaattacg caaagccagc ggcaatatcg
    22681 tcaccttcta tactggactg gcgttgttta attcggcgaa tgggcatcta caaacagaag
    22741 tggagccttt tgacgtccat ttccgtcacc tgagcgaggc ggagattgat aattacgtgc
    22801 gtaaagagca tcccctgcac tgcgcgggta gctttaagag tgaaggattt ggcattacgc
    22861 tgtttgagcg tttagagggg cgtgatccta acacgctggt tggtttaccg ctaatcgcgc
    22921 tgtgtcagat gttacgtcgg gaagggaaaa acccgctgat gggatagttt tttgcgccgg
    22981 atgcggttac gtcgcatccg gcaatcggtg ctggcggcct gatgcggcgt aaacgcctta
    23041 tcaggccaac cgcccattat caggtttata tgcttgtatt agcgcgcgtt acgcagcttt
    23101 tgcaaacaac gcttcaaacc ttcatccatc ggcgcttcaa tacgcatcac ctcaccggtc
    23161 cccggatgag taaacttcaa cgctgcagcg tgcaggaaca ggcgatttaa tcccgtgcct
    23221 gcttcagtga gctgtctgtc aaattcacgg tcaccgtagc gatcgtcaaa ggcaatcgga
    23281 tgacccgcat attgtgtatg cacacggatc tgatgagtac gccctgttac cgggctacaa
    23341 cgcaccaggg tggcaaatgc atagcgttct tccactttaa agcgtgtttc cgacggtttg
    23401 ccttcctgac tcacacgcac aatacgttcg ccgctttgca gaatattttt cagtaacggc
    23461 gcttgaacgc tcttcacatg cgactgccac tgaccgcgca ccagcgccag gtaatctttt
    23521 tgcatccctt tttcacgtaa ttgctcatgc agagaacgca acgccgagcg ttttttcgct
    23581 accagcaaca cacctgaggt gtcccggtca agacgatgaa ccagttcaag gaaccgcgct
    23641 tccgggcgca acgcccgcaa accttcaata acgccgaagc ttaaaccact gccgccatgt
    23701 accgccgtac cggaaggttt attcagcacc aggatatgat catcttcata taagatgacg
    23761 tccgccagcg ccgccacctt ttgcagatgt ggcgaaaccg cctcttcttc ccgctcagca
    23821 acgcgaaccg gtgggatacg cacctcatca cccgcttcga gtttatattc aggcttaata
    23881 cgttttttgt tcacccgcac ttcgccttta cgcaaaatac ggtaaatcat actttttggt
    23941 acgcctttca attgggtacg caaaaagttg tcgatacgtt gccccgcttc gtcagcggtg
    24001 atagcaacaa tttttacgga tggagtctct gttttcatgg tgggcgattc taattagcca
    24061 acaggattcg cgccactcat ttttctatgc ttatatttag ttttgcacct tattacttca
    24121 ctaggtgatc actttattga tggttattaa accaatcacc agcaagaagt gaaaaaactg
    24181 tgagtaagcg ggtgataaat ggtaaaagtc atcttgctat aacaaggctt gcagtggaat
    24241 aatgaggccg tttccgtgtc catccttgtt aaaacaagaa attttacgga ataacccatt
    24301 ttgcccgacc gatcatccac gcagcaatgg cgtaagacgt attgatcttt caggcagtta
    24361 gcgggctgcg ggttgcagtc cttaccggta gatggaaata tttctggaga gtaataccca
    24421 gtctgtttct ctgataattg cgctgttttt ccgcatgaaa aacgggcaac cgacactctg
    24481 cgcctctttg agctgacgat aaccgtgagg ttggcgacgc gactagacac gaggccatcg
    24541 gttcacaccc ggaaaggcgt tactttgccc gcagcttagt cgtcaatgta agaataatga
    24601 gtaagttacg atgaaaagaa tgttaatcaa cgcaactcag caggaagagt tgcgcgttgc
    24661 ccttgtagat gggcagcgtc tgtatgacct ggatatcgaa agtccagggc acgagcagaa
    24721 aaaggcaaac atctacaaag gtaaaatcac ccgcattgaa ccgagtctgg aagctgcttt
    24781 tgttgattac ggcgctgaac gtcacggttt cctcccacta aaagaaattg cccgcgaata
    24841 tttccctgct aactacagtg ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg
    24901 tcaggaagtc attgttcaga tcgataaaga agagcgcggc aacaaaggcg cggcattaac
    24961 cacctttatc agtctggcgg gtagctatct ggttctgatg ccgaacaacc cgcgcgcggg
    25021 cggtatttct cgtcgtatcg aaggcgacga ccgtaccgaa ttaaaagaag cactggcaag
    25081 ccttgaactg ccggaaggca tggggcttat cgtgcgcacc gctggcgtcg gcaaatctgc
    25141 tgaggcgctg caatgggatt taagcttccg tctgaaacac tgggaagcca tcaaaaaagc
    25201 cgctgaaagc cgcccggctc cgttcctgat tcatcaggag agcaacgtaa tcgttcgcgc
    25261 attccgcgat tacttacgcc aggacatcgg tgaaatcctt atcgataacc cgaaagtgct
    25321 cgaactggca cgtcagcata tcgctgcatt aggccgcccg gatttcagca gcaaaatcaa
    25381 actgtacacc ggcgagatcc cgctgttcag ccactaccag atcgagtcgc agatcgagtc
    25441 cgccttccag cgtgaagttc gtctgccatc cggtggttcg attgttatcg acagcaccga
    25501 agcgttaacg gccatcgaca tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga
    25561 aaccgcgttt aacactaacc tcgaagctgc cgatgagatt gctcgtcagc tgcgcctgcg
    25621 tgacctcggc ggcctgattg ttatcgactt catcgacatg acgcctgtac gccaccagcg
    25681 tgcggtagaa aatcgtctgc gtgaagcggt gcgtcaggac cgtgcgcgta ttcaaatcag
    25741 ccatatttct cgctttggcc tgctggaaat gtcccgtcag cgtctgagcc catcactggg
    25801 tgaatccagc catcacgtct gcccgcgctg ctccggtact ggtaccgtgc gtgacaacga
    25861 atcgttgtcg ctctctattc tgcgtctgat cgaagaagaa gcgctgaaag agaacactca
    25921 ggaagttcac gccattgttc ctgtgccaat cgcgtcttac ctgctgaatg aaaaacgttc
    25981 tgcagtgaat gcgattgaaa ctcgtcagga cggcgtgcgc tgtgtgattg tgccaaacga
    26041 tcagatggaa accccgcact accatgtgct gcgcgtgcgt aaaggggaag aaactccaac
    26101 cttaagctac atgctgccga agctgcatga agaagcgatg gcgctgccgt ctgaagaaga
    26161 gttcgctgaa cgtaagcgtc cggaacaacc tgcgctggca acctttgcca tgccggatgt
    26221 gccgccagct ccaaccccag ctgaacctgc cgcgcctgtt gtagccccag cacctaaagc
    26281 tgcaacggca acaccagcat ctcctgcaca accagggttg ttgagccgct tcttcggcgc
    26341 actgaaagcg ctgttcagcg gtggtgaaga aaccaaaccg gccgagcaac cagcaccgaa
    26401 agcagaagcg aaaccggaac gtcaacagga tcgtcgcaag cctcgtcaga acaaccgccg
    26461 tgaccgtaat gagcgccgcg atacccgtag tgaacgtaca gaaggcagcg ataatcgcga
    26521 agaaaaccgt cgtaatcgtc gccaggcaca gcagcagact gtcgagacgc gtgagagccg
    26581 tcagcaggct gaggtaacgg aaaaagcgcg taccaccgac gagcagcaag cgccacgtcg
    26641 tgaacgtagc cgccgccgta acgatgataa acgtcaggcg caacaagaag cgaaggcgct
    26701 gaatgttgaa gagcaatctg ttcaggaaac cgaacaggaa gaacgtgtac gtccggttca
    26761 gccgcgtcgt aaacagcgtc agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga
    26821 agaagcggta gtcgcaccgg tggttgaaga aactgtcgca gccgaaccaa ttgttcagga
    26881 agcggcagcc ccacgcacag aattggtgaa agttccgctg ccagtcgtag cgcaaactgc
    26941 accagaacag caagaagaga acaatgctga taaccgtgac aacggtggca tgccgcgtcg
    27001 ttctcgccgc tcgcctcgtc acctgcgcgt aagtggtcag cgtcgtcgtc gctatcgtga
    27061 cgagcgttat ccaacccagt cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact
    27121 ggcctctggc aaagtctgga tccgctatcc aattgtacgt ccgcaagatg tacaggttga
    27181 agagcagcgc gaacaggaag aagtacaagt gcagccgatg gtgactgagg tccctgtcgc
    27241 cgccgctgcc gaacctgttg ttagcgcgcc agttgttgaa gaagtggctg aagtcgtaga
    27301 agcccccgtt caggttgccg aaccgcaacc gaaagtggtt gaaacgacgc atcctgaagt
    27361 gattgccgcc gcggtaactg aacagccgca ggtgattacc gagtctgatg ttgccgtagc
    27421 ccaggaagtt gcagaacacg cagaaccggt ggttgaaccg caggaagaga cggcagatat
    27481 tgaagaagtt gccgaaactg ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc
    27541 tgccgcgcca gtcgtcgctg aagtcgcagc agaagttgaa acagtagccg cggttgaacc
    27601 agagatcacc gttgagcata accacgctac cgcgccaatg acgcgcgctc cggcaccgga
    27661 atatgttccg gaggcaccgc gtcacagtga ctggcagcgc cctactttcg ccttcgaagg
    27721 taaaggtgcc gcaggtggtc atacggcaac gcatcatgcc tctgccgctc ctgcgcgtcc
    27781 gcaacctgtt gagtaataat tagctcaagc aatcaagccc tggtaactgc cagggctttt
    27841 ttatttcatc tttgaatcaa catattaaaa ttcatcaaat ggcatccttg ctaaccaaca
    27901 atacaaaata ggcaaaaaaa tgcccgcccc ggcgatccca gagcaggcag acaaaacata
    27961 cccagtttca tgatatgttt taaagcgaaa ttatttgttg agctggaaga gcgacaatcc
    28021 ctgcatatcg gtaaatgctt tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga
    28081 tgaaatagtt gcattccagt ccacatcaac cagatcgctc atctgctgcg tttgccctaa
    28141 agcgcgatcg ctacctaatg aatcaagcga ctccagttcg ttcagctgcg tgcctaattc
    28201 cgcgcgaaca gtcagcacat tgttcagcga gtttttcagt ccgcggttgg ttttatccag
    28261 tgccgcagcg gcggtttctt tatccgcttc gctatccgcg accggcgttt tcagggctgc
    28321 gatggcgcta tccagcatgg caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc
    28381 taccgcgttg ctggtaatac tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga
    28441 acgcgaagca tcgacctgtt gtttaatact ttctgttccg ccgacataat caccgtcagc
    28501 ctcactaaac ggcgcagtct ctgttttata accggcaaaa atgtagcgcc cgttaccgtc
    28561 agtggtgttc gccagattca gtaactgatc acgaagcccc tgaatatccg tagccagtga
    28621 ggcccggtca tcgtcactca aggtgccatt gctggcgtag acaatttttt cctgagcatt
    28681 ctggatagca gtggtgacct ggctaagcac actctcttcc agtgacactt tttgagtggc
    28741 gaaagtacgc gccagcgtgt actggctgtt ttgtgcctgt gccagggaga gaacaacagc
    28801 ttgtgatgca gcaatgggat catctgaagg gttaacgact cgcttacccg tcgacatctg
    28861 ttcgccgtac ttcatccatt ctgcctgaga attggtgatg ccacgcatgt tttgctggta
    28921 catcatctgg gtactgaaac gcattttatc tccccttagc gaatgttaat cagcgcatca
    28981 aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt
    29041 tgcagatttc cgtactcctc atcgagattg acaccggaaa tcgactgctg ctgattggaa
    29101 agctgcgtca ccacattacc ttgcgtggcg ctactggttt tcaacgtcgc ggttttattc
    29161 ccgatatcac tcactaatga ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt
    29221 ttactgttgc tttgcagatc cagcagggcc tgaccgttgc ggttgtcgct atcacccgca
    29281 tcttcttcgc tcgccatggc gattttcgct tcgtcagtga ttaatacatc catgttgacg
    29341 atggcgtcac ttaccggttt cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac
    29401 gtcaactcca gaccatcaaa tgctacttta ccgttggcat ccggcgtcac cgtaaaagtg
    29461 gtattgctgg caaggcgggt gacctgccac tgattattat cgaacgagat tttgtaatct
    29521 gtcgccagta ccgcggaggc atcagttacc gtggcaccga tcgcaacgtc accgttgttt
    29581 ttcgtgtttt gcagaaccgc gggcttaccg atagcaaaga aatctttacc ggcatcgccg
    29641 ttggcatcaa atccggcttt gtgttgggtg ttgaaagccc cggcaaatgc cagcgccagt
    29701 tgtccgagcg tattacgcgt ctggtccaga tcctgagaac ggaatgtcag aatgccgccc
    29761 agcgacccgg tattcagtaa tttctccggg atctcaatat tgccagccgt cccatcaata
    29821 taagcgacag tcgtacgcga agggtcggcg gtggaaggaa ctgccgccag ctgccgcgcc
    29881 gtacttccct gaaccagtga gtagccattg gccatcgtga tgttataagt gccaccatcc
    29941 tgaacgctaa cttctacgcc aacaatctgg tttaattcac tcaccagttg atcgcgttga
    30001 tccagcagat tgttaggtga cgcccctgcc cccacgcctg tcagacgcga gatttgatcg
    30061 ttcaggctgg cgatttgttt agcgtaattg ttgatctgat caacactggc acctatcgcg
    30121 atattgacct gtttgtcctg gtcgcgcaga tattgatcgg tggttttaaa ctgattcacc
    30181 aacccttctg atttcccaat cagcgcctgg cgcgctgccg ggtcttccgc gttacttacc
    30241 agcgtttgca ggcttgtgaa gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg
    30301 gagagcatat tgtcgatttt cgacatctgc tcatagcggg cagtcaggcc gctactttgc
    30361 gtctgcgccg cacgtaactg gttggtaata aatgcatcat actcacgctg cacaccagaa
    30421 acgtagacgc cattgccaac ccagccgcca gcgcccaacg tgctattggc ctgcgccata
    30481 atagtggttt ggcgggtata tccggcaacg ttatagctgg agatattatt acttgccgta
    30541 tttaacgccg cctgggccgc gttcagtccg ctcatggcgt tattgatcaa gctggacatg
    30601 gaggttcctt ataagccttc aattacagag tattatcggc agcgacccgc cggacttgag
    30661 ttattcagaa cagattatca atgttcatac tgtaggtttt gctcactttg tcgcttatcg
    30721 atttcatctg ctgaatcatg ttggtgagtt tgcgggcata gtgaggatcg gtggcatagc
    30781 ccgcgtcctg tagggcctgc gcccccagct ccgcactcgc ggcggtcgtc acggcagcgt
    30841 agcgcggatt acgtgttaac agcccaacgt aatccgacaa tgcttccaga tacgagctgt
    30901 agacccgaaa tttcgctttt actttcttcg cttcgccgtt ttcatattca gtcgtggtga
    30961 tttcagtgac tggccctttc cagttgccag aggctttgac gccaaacagg ttataactag
    31021 gctcgccgtt ttcgcggcgg atttgccgtt gcccccagcc agattcaagc gccgcctgag
    31081 cgaggatcaa atgatgtggc acaccgcttt gctggcttgc cagttgggcg ggcagtgaga
    31141 gttgcgcgag gaatgcttta ctgttacccg gcaacgaatc atcgtagtta tgtggtacgg
    31201 ccttttgcac cagctgagaa agcgcctgat tttgataacg caccacagtt tcgagcggga
    31261 atttcatcgg tgctgctggc gtgggctcct ctggcaatgg ttgttctggc gtcatctgtt
    31321 taaccatcat ctctgcaagc cccagacctt tgcccgccgt catctgttgg gcaatctgct
    31381 ggtcatacat actggtatac aggcgagtgt gctcgctgct gaacaggcca tcttttggta
    31441 aagcgtcgcg catgcttttc aacatcatct gcacgaacat cccttccacc tggcgggcca
    31501 ccggacggat atttgccgct ggatcttcgc cagctttcgc ctttagttcg ttgagtgatt
    31561 gcgcatccca ggccgcactt gccagtagtt tgctgtcgct gatcatcaga tgatttccag
    31621 ttttgcccgc agacatcccg cactttgcat tgattgcaaa atagacatca gatccatcgg
    31681 cgtagcgccc agcgcattga gcgcgcgcac cacgttattg aggctggcgc tggaacgtac
    31741 gctttgcagc gaaccgccgc tctggcgtaa atcgatctgc gtttgtggag taaccacagt
    31801 ctgtccacca ccaaacggtg tatctggctg gctgacattg gcctgacgat tgaccgtaac
    31861 cgagagattc ccctgcgcta ctgcgcagct gtcgagggtc acttcgcgat tcatcaccac
    31921 cgagccagtg cgcgagttaa tcactacttt agcgtcctgc ggggtgacat taacctgcat
    31981 attctggata tcggcaagga aacggacctg ggaactgttg ccacttggta cgcgcacctg
    32041 aatagtccgc gcatctaacg cggtggcgct gccatatcca cgcacgcggt tgatggtgtc
    32101 agcgatttgc tgcgccatgc tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc
    32161 gacgccaaac tggctgggca attcacgttc aataaccgca ccattggtga tccgcccacc
    32221 gttcagttgg ttcacctgaa cactgctacc gccagcggag gctccagcgc caccaaccag
    32281 aatattgccc tgcgccagcg catacacctg actgtcaacg cccttaagcg gtgtcatcaa
    32341 caacgtaccg ccacgcaagc ttttggcatt acccatggaa gaaaccacca catcgatggt
    32401 ttgcccctgg cgtccaaacg gcggaagtga cgccgtcacc attaccgcag cgacgttttt
    32461 tagctgcata ttggtgcccg tcggaacggt aattcccagc tgtgagagca tgttattaag
    32521 cgtttgtgtg gtaaacggcg tctgggttgt ctggtcaccg gttccatcca gccccaccac
    32581 caggccatag ccaatcagtg agttttgcct taccccctga acactggtga gatcgcgaat
    32641 acgctcagcc tgagccgccg tgataaccag tagaagaatt aatgcagaga gaaatttaat
    32701 caccacagcc tcacttacat tggcgacagg ttaaggaaga aacgctgcaa ccagcccata
    32761 ttttgcgctt cgttaatgta gccattgcct acgtattcaa tgcgcgcatc cgccacctga
    32821 gtagatggta cggtattgct gccgctgata gtgcgtggat taaccacgcc agagaagcga
    32881 ataaattcgg taccctgatt aatggcgatc tgtttttcac ccaccacatg caggttgccg
    32941 ttgaccagta cctggtcaac cgtcaccgtc aacgtaccgc taaaggtatt gctggcattg
    33001 gccccgcctt ttccgttgaa cgtgttacca ccggaggctt cgacatcggc acgagcgtta
    33061 ccaaacaacc cctgcaaata gcgcggcaca gtatcaaagc caaaattagt tttaccgtca
    33121 cggctggcat tcgcagagga gcttttgctg gcgctgacgt tctcctgcaa cacgatagtc
    33181 agcgtatcgc caatattgcg tggtcgacga tcttcaaaca gcggttgata gccatagtta
    33241 atcggctgag cagactggaa aatagacccg ttggcgacgg gcgtcggacc gggaaccggt
    33301 tgtgcactgg tcgccccctg taccagcggc gtggagggta tccaggcaca gccggttagt
    33361 gaaagcacca acaagctgga aatggcataa gtatgcgcag cgtttttttg catggctatc
    33421 atcttcaaaa atcagtaaac cggtgaacct gccaccggtt aagccttaga gttgcgtcag
    33481 tttttgcagc atctgatcgg tggtggacac cgctttactg ttgatttcgt aagcgcgttg
    33541 cacctgaatc atattgacca gttcttccgc cacgttgacg ttagacgttt caacataccc
    33601 ttgatacagc agtcccgcgc cgttcaggcc cggcgtgctt tcgttcggtg caccggagga
    33661 ttgcgtttcg gtgtagaggt tttcgccaat gctctccagt ccggtgtcat tcataaaggt
    33721 ggtgagattg agctgcccaa cctgaaccgg agctgcctgg ccttgttggg ttacgctgac
    33781 cacgccatca cgaccgatgg tgatacttaa cgcattcgcc ggaatggtga tcgctggctg
    33841 cacctgaaaa ccaccggccg tcaccagctg cccgttctga tccacctgga aagagccgtc
    33901 acgggtatag gctgatgagc catctggcaa catcacctgg aaaaagccct gccctttaat
    33961 cgcgacgtct ttactgttat tggtttgcga caggttgccc tggctgtgta aacgttccgt
    34021 ggcgaccggg cgtacccccg taccgatttg taatccggat ggtaaggtgg tttgttcgga
    34081 agactgtgcc cccggctggc gaatagtttg ataaagcaga tcttcaaaca ccgcgcgctg
    34141 acgcttaaaa ccgttggtac taacgtttgc caggttgttg gcaataacgt ccatattggt
    34201 ttgctgggcg tcaaggcccg ttttggcgat ccataatgaa ctgatcataa aatgtcctgt
    34261 agtggattag tgacaaagtg catatgtctt cttgccggat gcggcgtaaa cgccttatcc
    34321 ggcctacaag agctcgctaa atcgataaat tgcgattgcc cccatagacc cgataaactt
    34381 gcgcataggg caacttatac ttgtcatgta tcctttcaat taactcatcg acagcagttg
    34441 gttggcacgg cctgcgttat catcgacgct gctgatcacc ttcatctgca tttcaaaacg
    34501 ccgtgcgctg gcaatcatgt cgctcattgc cgcaacggca ttgacgttac tgccttccag
    34561 aacccccgac atcacacgca aggttggatc tgccagcagt accggcccac gcgtagcctg
    34621 gctttctgcg cttaaacgaa aaatgccatc gtcaccgcgc tggacttcgc tgcccgtggc
    34681 tttcaccagc ttcagacgcc ccactggcgc aaccgtattt gccggatcgc ccggattcag
    34741 cgccgagatt gtgccatcgg cagcaatagt gatttccgcc ccttccggca cagcaattgg
    34801 ccccgcctcg cctaccaccg gatgtccctg aattgtcagt tgcccggtgg gatcaacctg
    34861 aatgctgcca ttacgcgtat acccttcact gccgtcagcg gtctgcacgg ccagccaacc
    34921 atcctgctgc aacgcaacgt ccagcgggcg cgaggtgtaa tccatcttgc cgggtgtcat
    34981 gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac
    35041 cggcactgcg cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag
    35101 attactggcg gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcggtata
    35161 aattgcgtga tccattgagc tatcccgtca gcgattagcg taagttaacc agcgtattga
    35221 ggatctggtc ctgggttttg atggtctggg cgttagactg atagttacgc tgggcaacga
    35281 tcatattgac cagttcttta ctgagatcga cgttggacgc ttccagcgca ccgttggtca
    35341 gtgtgccaaa gttgcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtag
    35401 cggaccagac gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta
    35461 caatctgccc cagcagttgg gtttgttcgt tggaatagtt gccgacaacc gtaccgtcat
    35521 cattgatttg ataactcacc agatcgcccg gtttgtagcc gttctgggtg gttgccacaa
    35581 tgttgttagc gccggtattt tgctgcatgg agttgaggaa gctcaggcta aacgtggcgg
    35641 gatctgcgcc gttaattgcg ccagtggaaa tattcgctgt tggatttgag gtcagaacgc
    35701 cattggcatt aaacaccagt gttgtcgctg tcttcgcaat gctgtttgga tcactactat
    35761 cctgggtata gacctgccag ttattatccc cggtcttcac aaagtagacg ctcatgtcat
    35821 gagcattacc ctgactatcg aaaacagtca ccgaaccttt tttgttatag ctatccgcat
    35881 tgctggcatc aaatgtgtta acagagggaa gcggatcact ggaattcagg ttgatctgca
    35941 tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt cgggatcgaa atattggtcg
    36001 gattcgcccc ttgctgaata gtcggcggcg taccggttgc cgggtacccc gtcagctgta
    36061 aaccttgcat attcaccagg ttacggtttt catccagctt aaattgtccg ttacggctgt
    36121 agaacaccga accattgctg tctaccagac ggaaaaaacc gttctggctg atagcaacgt
    36181 ccagaccacg cccggtgttg gtggtcgtgc catcggtaaa gtcctgagtg ataccagcaa
    36241 cttttacccc cagtcccact ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg
    36301 atttaaagcc gtaggtggcg gagttggcga tattgttgcc aataacatcg aggttggtgg
    36361 cggcaacgtt taatccgcta accgcttgag aaaaggccat gactgactcc tgaaatgtga
    36421 aggcttaaat tatctgccgt acttcgtcga gggtagtggt gccgtaagtg ccaagatcca
    36481 gcgtattacc gttgttgccg cggatcaccc cctgcaccag agcaaactgc agcggttggg
    36541 caaccagttg tgtaccaccg ttactggcgc taatcgctac attgtaagaa ccgttcggcg
    36601 cagtgctgcc gtcagtcaac gtaccgtccc aggtgaaact gtggactccg gcagtcagtt
    36661 caccgatatc aatggtacgg acgaccgcgc cgtttttatc ggtgatggtg gccgtcactt
    36721 tgtctgctgc ctgttgcaac tcaacaccaa acggcgtggt cgtggtcaca gccccttctt
    36781 cactgccggt tcccgcaaga acagtggtac cggggatcat cacgccgtga ccaatcaggt
    36841 tactggcctg taacgactgg ctgttatcaa tctgtccgga aatagatcca agcgtggtat
    36901 tgagtttttc aatcccactg accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt
    36961 tttccattgg attggtcggg tcctggtttt tcagctgcgc caccaacaaa gtcagaaaac
    37021 tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact actgctggtg gtactgacgc
    37081 cggtattcgt cggatcggtg gtggttaccg caatggacat agctttctcc tttattgacc
    37141 gagcgtaagg gttttcagca tcatactttt caccgtgttg agcacttcaa cattggcctg
    37201 ataactgcgt gacgccgaca tggtgttaac catctctccg acgacatcaa cgtttggcat
    37261 tttcacgtag ccctttgcat ccgccagcgg attacccggt tcataaacca gtttgtccgg
    37321 ggcctgactt tctataacat cggcaacctt tacgccgcct gtcgcagcac ctggcgcagc
    37381 gttaacctgg aataccacct gttttgcccg atatggctgt ccatcgggac cggtcacgct
    37441 atcagcattc gccagattac tggccgccac gttcaggcgc tgggactggg cagttaacgc
    37501 cgacccggcg atatcaaaaa tattcagcag tgccatccgt taatttccgc tctgtaaaac
    37561 gttcatcatg cctttgattt gcccgctcaa cgcactaagg ctcatctggt attgcaggct
    37621 gttatcggca aactgggtgc gttcgcgatc catatcgacg gtattaccgt caagcgaagg
    37681 ttggtccgga atacggtatt gcagttctgc ggtaggaggc gtcagcgcct gcgccggaat
    37741 gtgttgcgtt gaggtcatcg tcagtgcaac cacactggtt gcatcccgtc cacgttgcat
    37801 gactttttta agttcactgg caaaatcgat atcgcgcgcc tgataaccag gggtatcggc
    37861 attggcgatg tttgctgcca gcacttcctg acgctgggcg cgcagattga gcgcctcttg
    37921 ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct cctccgcagg tatcaaaatt
    37981 ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc ggaataaacg caaaatgggt
    38041 cgctatttat gccgttgatg gtcattgcgg acaggtacaa ttcacgttgt agaaatggct
    38101 gggggcgaaa atgctggcaa taaaacgtag cgtggcgatc atcgcgatac tcttcagtcc
    38161 gttaagtacg gcgagcaatc tcacatcgca attgcacaac ttttttagcg cccaactcgc
    38221 gggggtaagt gatgaggttc gtgtttctat tcgtacagcg cccaatctgc taccgccgtg
    38281 cgagcagcca ttgctttcga tgagcaataa ttcccgcctg tggggcaatg tgaatgtgtt
    38341 ggcacgctgc ggtaacgaca aacgatattt acaggttaat gtacaggcca caggaaatta
    38401 tgtggttgcc gcgatgccca ttgtgcgtgg aggaaagctg gaagctggca atgtcaaact
    38461 gaaacgcggc cggctggata ccttgccacc gcgtacggtg ctggatatca atcaacttgt
    38521 tgatgccgtt agcctgcgcg atctatcacc cgatcaacct atccagttaa cccactttcg
    38581 ccaggcatgg cgggtaaaag cgggacaacg tgtcaacgtg atcgccagcg gtgatgggtt
    38641 tagcgccaac gcagaaggtc aggcgctgaa caatgctgcc gtcgcacaga atgcgcgggt
    38701 gcgcatgata tcgggacaag tggtcagcgg cgttgttgat gcagatggga atattctcat
    38761 aaacctgtaa gctgttaaag attacccgtc ccttgccgat aaataagcaa cacatgataa
    38821 aagcgccctc aatgaggaat aaaccatgag tattgatcgc acttcgcctc tgaagccagt
    38881 aagcaccgtt caaccgcgcg aaaccactga cgcgccggta acaaacaccc gagcggcaaa
    38941 aacaacagcc tccaccagca ccagtgtgac gttaagcgac gcgcaagcaa aactgatgca
    39001 acccggcagc agcgatatca atcttgaacg tgtcgaagcg ttaaaactgg cgattcgtaa
    39061 cggtgaacta aaaatggaca ccggcaaaat tgccgatgcg ctgatcaacg aagcgcagca
    39121 agacttgcag agtaattgat cgtatgacgc gtcttgcaga gatcctcgac cagatgtccg
    39181 ctgtgcttaa cgatctcaaa acggtaatgg atcaagagca gcaacatctc tctatggggc
    39241 agatcaacgg cagccagttg caatggatta cagaacaaaa aagctcactg ctggcgacgc
    39301 tggattacct cgaacagtta cgcaggaaag aacccaatac agcaaatagc gttgatatta
    39361 gtcaacgctg gcaggaaatt actggcaaaa cgcagcaact acgccaactg aatcaacata
    39421 acggctggtt actggaagga cagattgaac gcaatcaaca ggcgctggaa atgttgaaac
    39481 cgcatcagga accgacgctg tatggggcga acggtcagac ctcaacaacc catcgcggca
    39541 gtaaaaagat ttcgatctga agattaactc cggcctgcac tgcaggccgg aatgtatcgt
    39601 tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca gtactgccag tgcagcgaag
    39661 tacgcggcaa tccccgccag cacgaccgcc attaaacgca gtaaacgcca gggcatggta
    39721 cccaatgacc actccggcat gatatgtaac atacctaaaa gcacgccaga catcaccagt
    39781 accgccacca ccagacgcaa cagaaacgcc gtccagccgg gttgcggggt aaagattttc
    39841 tgcttacgca actgccagta aagcagcgaa gcattcagac acgccgccag accaatagaa
    39901 agtgacagcc cggcatgttt caacggacca ataaacgcca ggttcatcaa ttgcgttaaa
    39961 atcagcgtaa cgatggcaat tttcactggc gttttaatgt cctggcggga ataaaagcca
    40021 ggagccaaca ctttcactac aatcaggcca atcaaaccca ccgagtaggc aattaacgcc
    40081 cgctgggtca tcagcgcatc aaaggcggta aatttaccgt actggaacag cgaaacggtt
    40141 aacggaccgg aaagaatgcc caacgcaacc gcacttggca gcgccaacag gaaacaaaga
    40201 cgcagccccc agtccatcaa acggttgtat tcatcatgat tgccactggc aaaacttttc
    40261 gacagcgacg gtagcaaaat ggtgccaagc gccacgccca gcacaccgga cggaaactcc
    40321 attaagcggt cggcgtaata catccacgac accgaaccgg aagcaagaaa cgaggcaaaa
    40381 atggtgttga tgattaagga gatctggctc acagagacgc caaggattgc cggtcccatc
    40441 tgtttcacca cacgcattgc tccggcatcg tggaagttaa tgcgtggcaa aaccagcatg
    40501 ccgatcttct tcagatgagg tagctgatac accagctgca ggatgccgcc gaccgtaaca
    40561 gcccaggcca gcgccagcac cggtggatta aagtacggcg cggcaaacag cgcaaaacca
    40621 atcatgctga tattgagcaa tgtcggggca aacgccggaa ttgagaagcg gttccaggta
    40681 ttcagaatcg ctcccaccag cgacgccagg gagatcagca agatataggg aaaggttatc
    40741 tttagtagct ggctggtcag ggcaaatttg tcggcagtgt cagcgaagcc aggcgcggtc
    40801 accatgatca cccacggtgc agcgagcatg ccagcgaccg tcacaaccgc cagcgcaagt
    40861 gtcagcaggc cagaaacata agagacaaag acccgcgtgg cgtcttcacc ctgcttgctt
    40921 ttatattctg ccagaatcgg tacaaatgcc tgggaaaatg ccccttcggc aaagatacgg
    40981 cgcaacaagt taggaagttt aaaagcgacg aaaaaggcgt cggttgccat ccctgcgcca
    41041 aagattctgg cgacaattgc gtcacgtgcg aagccaagca cacgcgaaaa catggtcatc
    41101 gagctgacgg cggccagcga ttttaataaa ttcatcggtg ttctaatcca gacccacggg
    41161 caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt ctacctggat tatggtgaat
    41221 tgctaccgcc agatgttaca gggtttattc actcatcgca tcgcgccaga tcttgtcaac
    41281 gatacgttgc gccagcacgg cctgttcgcc ggcggtttgc ggaactgtct gattttgcac
    41341 gcattcaatg aagtgccgcg cacagccgac aaatccacgt tgctcaagcg tactctgcca
    41401 accaggaatc ggtttatgca ctacgccctg cccgcgctcc tcacgccatt cgcgcatatc
    41461 cgtaatgtcg atgagcgcac catcagtcac ggcctgcacg gtttcacgct gacttccggc
    41521 acggcgatgc atacaggtgg tgatctgcaa aggaccagcc gaaaaatggt gctcggcaaa
    41581 cagcatttcg ccagcgtcgt tagtcagtaa cgtaccgcca tccagagagg cttggccacc
    41641 tgacaaccac agcgcggtat caaccacatg caaataatca tccagcaatg tgaaataaag
    41701 atcgtgtggc ccgacgctat tgctacgatg tttatccatt cttagcgagg acgcggtggc
    41761 gagttgcgtt tttaactcac cgtagagtgg tgcgaaacga cggttaaaac cgaccatcaa
    41821 cgtcagtttt ttccgcgccg ccagttccac cagccgttca gcatcgcgca gattttctgc
    41881 cagcggttta tcgacacaga catgtacccc cgcattgagt aacgtactga ccacatcaaa
    41941 gtggctggcg gtgctggaat gcacaaaaac cgcatcgcaa ctggcggcaa ggctggataa
    42001 cgaatcagca taaggaatgc gccagctgtc acaaattggc agggctttgg cgcgcgtagg
    42061 cgaccacgca ccttgtaacg tccagtcaga cgctgccgcc agcaccggta accacgcttt
    42121 ttgcgcgatg ccgcctaacc ccactacgcc gatacgtaat tttttcactt taatctccca
    42181 gatgggccag caacgaatca agacgctgtt tcagttctgc cacttcgatt tccagggctt
    42241 cgacgcgggc ctgtaaatca ccgtcagctg cgttcgacat atccgttacc gccggcggat
    42301 tctcaacctc accactgaaa agatgcatgt agcggctttc gcgtttaccc ggttcgcggg
    42361 ccagacgcac cacaaaagga ccatcttcgc gactcgccag ttgttccagc gtcgactcca
    42421 cttccgccat atcgctgaat tcatacattc gcgcggcgcg gctgcgcagt tcccccggcg
    42481 tctgggcacc acgcaataac aacgtggtga ttaacgccac ttctgcggcg ctcagtttca
    42541 gatcgccaaa ttctgaatta caaaaacgtt gctcatattt ggtgacccga ttaccaaaac
    42601 cgctcaccgt gcgtagataa tgacgtttga ccagattatc cagctgttcc tgcacttcag
    42661 attcactcag attcataacc ggttcacggt tcgttttctg attacaggcc gtgactacac
    42721 catttacgga gagcgggtat tgctccggcg tggtcacctg tttttccagc aagcaaccaa
    42781 tcacgcgagc ttccatggca gtaagttgat atttcatgag attctcctta gcggccgggc
    42841 gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc gccattgtcc atcaatcaac
    42901 agatagtctt tcgcatagcc ttctttttca aaacccagtc gcgccagtaa atcaccgctg
    42961 cgtttattgt gcggcatata attagccata atgcgatgaa tatgttgggt gcgctgcata
    43021 taacgaatgg ctgcgctcag agcttcaaac atgagtcctt tgccctgcca tttttgccca
    43081 atcgaataac cgagatagca ggcatgaaaa gagccgcgaa caacattgga aaaattggca
    43141 acgccaataa tctctttttc gtccgggtcg aataagccaa agtagaaagc tgaaccttgt
    43201 ttatgaaatt cgttaatcat ccctagcctg gcctgccagc ctgatggata acagtggctt
    43261 tcgtcgcgca ctggctccca gggcttgagg aaatggcgat tctctgcgta ataatccgcc
    43321 agacgccagg catcacgatc atgcaccaga cgcacgacca gtcggtctgt ggttaagcgc
    43381 acttttggca cgttactgcg atagccaaac attctatacc tactccttcc cgtaacgtct
    43441 ttaatacgct gtaagtatta ctatacctgc gcaaccagac cctgtgaaaa gtccagaatg
    43501 ccattttctg cattattcac atatttgata aaaactctct atcaaagcga cgttttgata
    43561 aaaaaatatt gtccccgaag tgatgggaaa agtcttagcg gcaatgcaga atagaaagtg
    43621 ctcatctttt atttcccggg aggggaaatg tcccgcgtgt cgcaggcgag gaacctgggt
    43681 aaatatttcc tgctcatcga taatatgctg gtcgtgctgg ggttctttgt tgtcttcccg
    43741 ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg ccgtcatggt cggtattgcc
    43801 ctcggtctac gccagtttat tcagcaaggt ctgggtattt tcggcggcgc aattgccgac
    43861 cgctttggtg ccaaaccgat gattgtcacc ggtatgctga tgcgtgccgc cggattcgcc
    43921 acaatgggta tcgcccacga accgtggcta ctgtggtttt catgcctgct ttcgggactc
    43981 ggcggcacgt tgtttgatcc tccgcgttcg gcgctggtgg tgaaattaat ccgtccacag
    44041 cagcgtggtc gttttttctc gctgttgatg atgcaggaca gtgccggtgc ggtcattggc
    44101 gcattgttgg ggagctggct gttgcaatac gactttcgcc tggtctgcgc cacaggggca
    44161 gttttgtttg tgctgtgtgc agcgttcaat gcgtggttgt taccggcatg gaaactctcc
    44221 accgtacgca cgcccgttcg cgaaggcatg acccgcgtga tgcgtgacaa gcgttttgtc
    44281 acctatgtcc tgacgctggc gggttactac atgctggctg tacaagtgat gctgatgctg
    44341 ccaattatgg tcaacgacgt ggcgggcgcg ccctctgccg ttaaatggat gtatgccatt
    44401 gaagcgtgtc tgtcgttaac gttgctctac cctatcgccc gctggagtga aaagcatttt
    44461 cgtctggaac accggttaat ggctgggctg ttgataatgt cattaagcat gatgccagta
    44521 ggcatggtca gcggcctgca acaacttttc accctgattt gtctgtttta tatcgggtcg
    44581 atcattgccg agcctgcgcg tgaaacctta agtgcttcgc tggcagacgc aagagctcgc
    44641 ggcagctata tggggtttag ccgtctgggt ctggcgattg gcggcgctat tggttatatc
    44701 ggtggcggct ggctgtttga cctgggcaaa tcggcgcacc agccagagct tccgtggatg
    44761 atgctgggca ttattggcat cttcactttc cttgcgctgg gttggcagtt tagccagaaa
    44821 cgcgccgcgc gtcgtttgct tgaacgcgac gcctgatttg atactccccc ctgactcctc
    44881 catactgaaa gtgaactgac agccaaatgg aggagtcacg tgaagctata catttacgat
    44941 cactgccctt actgcctcaa agcccgcatg attttcggcc tgaaaaatat ccccgtcgaa
    45001 ttacatgttc tgctcaacga cgacgcagaa acacccaccc ggatggtcgg tcaaaaacag
    45061 gttcccattc tgcaaaaaga tgacagccgc tatatgccag aaagcatgga catcgttcac
    45121 tatgtcgata aactcgacgg caaaccgtta ctgaccggca aacgttcccc tgccattgaa
    45181 gagtggctgc gcaaggtcaa tggctacgcc aacaaactgc tgttgccgcg ttttgccaaa
    45241 tcggcatttg atgagttttc tactcctgcc gcgcgcaaat atttcgtcga caagaaagag
    45301 gccagcgcgg gtaattttgc cgacctgctg gcccactctg acggtctgat taagaatatc
    45361 agcgatgatt tacgtgcgct ggacaaactg atcgtcaaac cgaacgccgt gaatggcgaa
    45421 ctttcggaag atgatattca gctatttccg ctcctgcgta atctgacgct agtagccgga
    45481 atcaactggc caagccgcgt tgctgattac cgcgataata tggcgaaaca gacacaaatc
    45541 aatttgttat catcaatggc gatttaatcc tgacccgccg cggcagcctg tattcgcggg
    45601 ttgccgctgt tttcagtttt ttctgctggc gcgtttatcg ctggtgacac atgctgcgtt
    45661 gtgtcaggat attgacgcag atttcatgtc gaggactacc atgaacaagt ttttatttgc
    45721 cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat caactcaccc aatacaccat
    45781 caccgaacaa gaaattaacc agtcgcttgc gaaacataat aatttctcaa aagatatcgg
    45841 tttacccggc gtggctgacg cccatattgt tctgacaaac ctgaccagcc aaattggtcg
    45901 cgaagagccg aataaggtta ccctaaccgg agacgccaat ctggacatga actccctgtt
    45961 cggtagccag aaagcgacca tgaaactgaa gctgaaagcg ctgcccgtgt ttgataaaga
    46021 aaaaggcgcg atcttcctga aagagatgga agtggtcgat gcgacggtac aaccggaaaa
    46081 aatgcaaacg gtgatgcaaa cgttgcttcc ctatttaaac caggcattac gcaattactt
    46141 taaccagcaa cctgcttacg tcctgcgcga agatggcagc cagggcgaag caatggcgaa
    46201 gaaactggcg aaaggcattg aagtgaagcc aggcgaaatt gtcattccgt ttactgatta
    46261 atcacgaggg cgcattcgcg ccctttattt ttcgtgcaaa ggaaaacgtt tccgcttatc
    46321 ctttgtgtcc ggcaaaaaca tcccttcagc cggagcatag agattaatga ctgcaccatc
    46381 ccaggtatta aagatccgcc gcccagacga ctggcacctt cacctccgcg atggcgacat
    46441 gttaaaaact gtcgtgccgt ataccagcga aatttatgga cgggctatcg taatgcccaa
    46501 tctggctccg cccgtgacca ccgttgaggc tgccgtggcg tatcgccagc gcattcttga
    46561 cgccgtacct gccgggcacg atttcacccc gctgatgacc tgttatttaa cagattcgct
    46621 ggatcctaat gagctggagc gcggatttaa cgaaggcgtg ttcaccgctg caaaacttta
    46681 tccggcaaac gcaaccacta actccagcca tggcgttacg tcagttgacg caatcatgcc
    46741 gatacttgag cgcatggaaa aaatcggtat gccgctactg gtgcatggtg aagtaacaca
    46801 tgcagatatc gacatttttg atcgtgaagc gcgctttata gaaagcgtga tggaaccgct
    46861 gcgccagcgc ctgaccgcgc tgaaagtcgt ttttgagcat atcaccacca aagacgctgc
    46921 cgactatgtc cgtgacggaa atgaacggct ggctgccacc atcactccgc agcatctgat
    46981 gtttaaccgc aaccatatgc tggttggtgg cgtgcgtccg cacctgtatt gtctacccat
    47041 cctcaaacgc aatatccacc aacaggcatt gcgtgaactg gtcgccagcg gttttaatcg
    47101 tgtattcctc ggaacggatt ctgcgccaca tgcacgtcat cgcaaagaga gcagctgcgg
    47161 ctgcgcgggc tgtttcaacg ccccaactgc gctgggcagt tacgctaccg tctttgaaga
    47221 gatgaatgct ttgcagcact ttgaagcatt ctgttctgta aacggcccgc agttctatgg
    47281 cttgccggtc aacgacacat tcatcgaact ggtacgtgaa gagcaacagg ttgctgaaag
    47341 catcgcactg actgatgaca cgctggtgcc attcctcgcc ggagaaacgg tacgctggtc
    47401 cgttaaacaa taaaaatcgt agcttcctgt tgtcattagg ttattttacc tgtataaata
    47461 accagtatat tcaacagggg gctattatgc gaattgaagt caccatagcg aaaacttctc
    47521 cattgccagc tggggctatt gacgccctgg ctggcgaact ttcccgccgt attcagtatg
    47581 cgtttcctga taatgaaggc cacgtatcgg tacgttatgc cgcagcaaat aatttatcgg
    47641 ttattggcgc gacaaaagaa gataaacagc gcattagcga aattctccag gaaacgtggg
    47701 aaagcgccga tgactggttt gtcagcgaat aatatgcagt gatttttgtt tttgccgggt
    47761 cgccccggct tttttatttt acttgcgaat tgtgagtaca aaatttctac acttcctcaa
    47821 aataattgca caatatgcta aaaaaatatg aatgacatgc tgttttattg ttcatataaa
    47881 tcttaaaagc agaaatttat ctgctccccc ctttaaaaat tcggtgaata cccttactta
    47941 ttggtatact gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt
    48001 aacacgaatt agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc
    48061 gtagggtggg acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac
    48121 cagaccccaa ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc
    48181 actgatgttg ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc
    48241 ggtgatttcc aggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa
    48301 ggcacctcac cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc
    48361 tgcttaaacc atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc
    48421 ggttccgtag gtgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg
    48481 accgacgccc atatgccacc gcatcaacac ggcagccacc acggcgatac gcgattaatt
    48541 cgccatgctt atggtgaagg tgaaaagtat gtcccgctgg tcctccgcgc gcaaatgctg
    48601 tgggatgaac tttcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt
    48661 aacctcggtc cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg
    48721 caactcaacg ttgaaaagct cgacgcgcaa gggattatgg cccgctggcc agaaatacgc
    48781 gtcccggaca actacatcgg tttatttgaa actgattccg gttttttgcg cagcgaactg
    48841 gcgattaaaa cctggatcca actggcgaag gaagcgggct gtgcgcagct gttcaactgt
    48901 ccggtcaccg caattcgtca tgacgatgat ggcgtaacta ttgaaacggc tgacggtgag
    48961 tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct actcccggag
    49021 ctgcctgtcc agcccgtacg caaagtattt gcctggtatc aggccgatgg ccgctatagc
    49081 gtgaagaata aattcccggc atttaccggt gagctgccca atggcgatca atattatggt
    49141 tttccggcag aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat
    49201 tcagcagatg aacgtgttcc gtttgcggaa gtggtcagcg atggttcgga agccttcccg
    49261 ttcttgcgca atgtgttgcc gggtatcggc tgctgcctgt acggcgctgc ctgcacctat
    49321 gataattcgc ctgacgaaga ttttattatc gatactctac ccgcccacga taatacactg
    49381 ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct
    49441 gccgattttg cgcaagacaa aaaaagcgat tttgatttga caccattcag gctttcccgc
    49501 ttccaataat catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc
    49561 gtcggttggt gaactatctc attaataata ttcgcgagca tctgatgctt tatctttttc
    49621 tctggggact attagcaatt atggacctaa tctacgtgtt ttatttttag ttttccgcac
    49681 tgacataaac tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat
    49741 tcttcctgcc tctacagcac tcactagacc ttccagatgc ttaacccact aaacttatga
    49801 agattctttc attatta
//
LOCUS       DADTFG010000013        48867 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.013, whole genome shotgun sequence.
ACCESSION   DADTFG010000013 DADTFG010000000
VERSION     DADTFG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.013"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..602)
                     /locus_tag="J1138_000792"
     CDS             complement(<1..602)
                     /locus_tag="J1138_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBA8452631.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
     gene            complement(813..2210)
                     /gene="ccp"
                     /locus_tag="J1138_000793"
     CDS             complement(813..2210)
                     /gene="ccp"
                     /locus_tag="J1138_000793"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBA8452632.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2615..4264
                     /gene="treF"
                     /locus_tag="J1138_000794"
     CDS             2615..4264
                     /gene="treF"
                     /locus_tag="J1138_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HBA8452633.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSEKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4315..4917)
                     /locus_tag="J1138_000795"
     CDS             complement(4315..4917)
                     /locus_tag="J1138_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA8452634.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYRLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5365..6336
                     /locus_tag="J1138_000796"
     CDS             5365..6336
                     /locus_tag="J1138_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8452635.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKSLLLPRLSEFLYLHPGIELELSSHDRPVDILH
                     DGFDCVIRTGALPEDGVIARSLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGIAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            6385..7398
                     /gene="yhjD"
                     /locus_tag="J1138_000797"
     CDS             6385..7398
                     /gene="yhjD"
                     /locus_tag="J1138_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBA8452636.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7791..9113
                     /locus_tag="J1138_000798"
     CDS             7791..9113
                     /locus_tag="J1138_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA8452637.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMSVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9292..11352)
                     /locus_tag="J1138_000799"
     CDS             complement(9292..11352)
                     /locus_tag="J1138_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBA8452638.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQVFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11422..12189)
                     /gene="pdeH"
                     /locus_tag="J1138_000800"
     CDS             complement(11422..12189)
                     /gene="pdeH"
                     /locus_tag="J1138_000800"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBA8452639.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12421..13350
                     /locus_tag="J1138_000801"
     CDS             12421..13350
                     /locus_tag="J1138_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA8452640.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGIAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13446..14942)
                     /locus_tag="J1138_000802"
     CDS             complement(13446..14942)
                     /locus_tag="J1138_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBA8452641.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15162..16448)
                     /locus_tag="J1138_000803"
     CDS             complement(15162..16448)
                     /locus_tag="J1138_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBA8452642.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16631..18619)
                     /gene="hmsP"
                     /locus_tag="J1138_000804"
     CDS             complement(16631..18619)
                     /gene="hmsP"
                     /locus_tag="J1138_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBA8452643.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18701..22174)
                     /gene="bcsC"
                     /locus_tag="J1138_000805"
     CDS             complement(18701..22174)
                     /gene="bcsC"
                     /locus_tag="J1138_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HBA8452644.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAILELQQAVRANPKDSEALGALGQAYSQKGDRANAVVNLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRGQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPAIDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEALAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDTADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(22156..23262)
                     /gene="bcsZ"
                     /locus_tag="J1138_000806"
     CDS             complement(22156..23262)
                     /gene="bcsZ"
                     /locus_tag="J1138_000806"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBA8452645.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLTEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23269..25608)
                     /gene="bcsB"
                     /locus_tag="J1138_000807"
     CDS             complement(23269..25608)
                     /gene="bcsB"
                     /locus_tag="J1138_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBA8452646.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(25619..28237)
                     /gene="bcsA"
                     /locus_tag="J1138_000808"
     CDS             complement(25619..28237)
                     /gene="bcsA"
                     /locus_tag="J1138_000808"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBA8452647.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(28234..28986)
                     /gene="bcsQ"
                     /locus_tag="J1138_000809"
     CDS             complement(28234..28986)
                     /gene="bcsQ"
                     /locus_tag="J1138_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBA8452648.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWCDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEF
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28998..29186)
                     /locus_tag="J1138_000810"
     CDS             complement(28998..29186)
                     /locus_tag="J1138_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452649.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            29459..31030
                     /gene="bcsE"
                     /locus_tag="J1138_000811"
     CDS             29459..31030
                     /gene="bcsE"
                     /locus_tag="J1138_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBA8452650.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            31027..31218
                     /gene="bcsF"
                     /locus_tag="J1138_000812"
     CDS             31027..31218
                     /gene="bcsF"
                     /locus_tag="J1138_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBA8452651.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            31215..32894
                     /gene="bcsG"
                     /locus_tag="J1138_000813"
     CDS             31215..32894
                     /gene="bcsG"
                     /locus_tag="J1138_000813"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBA8452652.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDTFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQISGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32981..33088)
                     /locus_tag="J1138_000814"
     CDS             complement(32981..33088)
                     /locus_tag="J1138_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBA8452653.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            33564..34835
                     /locus_tag="J1138_000815"
     CDS             33564..34835
                     /locus_tag="J1138_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA8452654.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
     gene            complement(34865..35869)
                     /gene="dppF"
                     /locus_tag="J1138_000816"
     CDS             complement(34865..35869)
                     /gene="dppF"
                     /locus_tag="J1138_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBA8452655.1"
                     /translation="MSTHEATPQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRQEKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(35866..36849)
                     /gene="dppD"
                     /locus_tag="J1138_000817"
     CDS             complement(35866..36849)
                     /gene="dppD"
                     /locus_tag="J1138_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBA8452656.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(36860..37762)
                     /gene="dppC"
                     /locus_tag="J1138_000818"
     CDS             complement(36860..37762)
                     /gene="dppC"
                     /locus_tag="J1138_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBA8452657.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(37772..38791)
                     /gene="dppB"
                     /locus_tag="J1138_000819"
     CDS             complement(37772..38791)
                     /gene="dppB"
                     /locus_tag="J1138_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBA8452658.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(38942..40549)
                     /gene="dppA"
                     /locus_tag="J1138_000820"
     CDS             complement(38942..40549)
                     /gene="dppA"
                     /locus_tag="J1138_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBA8452659.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(41293..41369)
                     /locus_tag="J1138_000821"
     tRNA            complement(41293..41369)
                     /locus_tag="J1138_000821"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41333..41335),aa:Pro,seq:cgg)
     gene            complement(41461..43152)
                     /gene="eptB"
                     /locus_tag="J1138_000822"
     CDS             complement(41461..43152)
                     /gene="eptB"
                     /locus_tag="J1138_000822"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBA8452660.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEASAN"
     gene            complement(43405..43929)
                     /gene="lpfE"
                     /locus_tag="J1138_000823"
     CDS             complement(43405..43929)
                     /gene="lpfE"
                     /locus_tag="J1138_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HBA8452661.1"
                     /translation="MKRTILALCLLPFSFSSLSATTDLGAKGTLKFSLKISQGACELV
                     KDSVEVDMGLAVLKKPVRIGTELNPTTFSIGLKNCSEVVRAYVTMDGTPNADDPNLFA
                     LDAGGATGVGLKIKTSAGVQQFPKNTTPTPIEFAINGDGAHQLNYIASYVPLRADATI
                     GRADATVDFTVQYE"
     gene            complement(43935..44987)
                     /gene="lpfD"
                     /locus_tag="J1138_000824"
     CDS             complement(43935..44987)
                     /gene="lpfD"
                     /locus_tag="J1138_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HBA8452662.1"
                     /translation="MKIKNLLAGMVLLGTSTFTGNIWAADWGPCRTASGDPFIFVTSF
                     TKNIQNPTDNVTGQTYPDFYQWALGDKYSGVCECPSPNPTEARPTLYKTESTLAAGHN
                     STYFKITNNLEVSTRVYIANVGNVQVPFINKSNSQPGRECDQPTFGWTTGSKGQLSLY
                     IAKPFVGEQNIPQTIIVSVFGTKKENVYSSVPISQVLLSGKVTVTQGCELAAGTSLDI
                     DFGEYQAHDFKGRTGQSPQNVQKIQKELTFNCTNISDGVHIYLSLEGTPNAAYPSAIS
                     LGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEYVKRKVTTTITAYPVSTTGKLP
                     AAGDYSGVATMHVELD"
     gene            complement(44998..47529)
                     /gene="lpfC"
                     /locus_tag="J1138_000825"
     CDS             complement(44998..47529)
                     /gene="lpfC"
                     /locus_tag="J1138_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HBA8452663.1"
                     /translation="MHWMHLPLDHYRAHFSFSLLALTIASALPAYGGKFNPKFLENVQ
                     GIDQHVDLSVYDSPVGQQIPGKYRVFVFVNEEKMASRTLDFSTASEAQRKASGESLMP
                     CLSRVQLEEMGVRIDSFPALKILPPEACVAFDEIIPQATSRFDFNTQTLHLTFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGSGSSPDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGTL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKESDGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSGKPRFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKDEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSIAYTWTRSPEWDED
                     DRLWSFSLSIPLGGAWGSYRMTTDQNGKTSQQASVSGTLLEDRNLNYNVQQGYTSNGV
                     GNSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAIVRSRFDTRVGYRVLMSLKRANGNAVPFGATAALSDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGHEQAQRCRVPFRLPEKKDNSGIVMVNAVCDK"
     gene            complement(47552..48238)
                     /gene="lpfB"
                     /locus_tag="J1138_000826"
     CDS             complement(47552..48238)
                     /gene="lpfB"
                     /locus_tag="J1138_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone LpfB"
                     /protein_id="HBA8452664.1"
                     /translation="MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQMSINGQKIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGV
                     AGEMLTKSIN"
     gene            complement(48322..48846)
                     /gene="lpfA"
                     /locus_tag="J1138_000827"
     CDS             complement(48322..48846)
                     /gene="lpfA"
                     /locus_tag="J1138_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HBA8452665.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGSVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTTTGDKSAATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     ISVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVATKNAVTI
                     GYGNAEVDFNLTYN"
BASE COUNT        11734 a        13140 c        12030 g        11963 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata
       61 gggatctcac gcaactccac atcccagtag cgggcgaatt tatgccagca gatttgtacc
      121 ggaccgcaca ccaggtttgg tttatccgtt ggtttgcctg cggcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggtgca ggcgcatgcc acagatcggc aaccatgttt
      301 acgcaacgca ggtcgattgc agcggattgc ggatattctt ctttgtcgat ccagttttta
      361 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctgaaatgca
      481 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      541 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      601 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      661 agcaaggtat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      721 taatataaac atttaatatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      781 cccaaacact cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      841 taaaccccgt tcagactgtg caggaaagct acgatatcat ccacatcttc ctgcggcagc
      901 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      961 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
     1021 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttca
     1081 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
     1141 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
     1201 gccgtcagag cattctcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
     1261 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
     1321 tagacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1381 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1441 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1501 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct accatcgacg
     1561 cctcccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacga
     1621 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1681 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1741 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1801 cccgcccagt gtagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1861 tcaatcttat tcagatcgct ttgcgaaacg ggtttattag ccagcagagc cgcacgcacg
     1921 gcctcgaggt taaaagattt atatccaagc tttatgtcgt aatccatcaa ctgtttcgcg
     1981 ccgggaatat aataataggc aggtaattct gccgaaggcg tatggcaata atcgcatcct
     2041 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     2101 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     2161 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     2221 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     2281 agacaaagcc atcataaaaa acagggagat atgctttttg ataggaatca atcaattaca
     2341 tgaatataaa ctatcaataa gatagccagt gcctattacc cagatcagga atggcatatc
     2401 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2461 tttttataaa ttccattgat attagtgcgt tatatttttt ggcaggttaa taaacgtgat
     2521 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2581 ataattagcg acgaatttcg gaggtcggat ccttatgctc aatcagaaaa ttcaaaaccc
     2641 taatccagac gaactgatga tcgaggtcga tctctgctat gagctggacc cgtatgaatt
     2701 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2761 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcgga
     2821 gaaaattttc cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga
     2881 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2941 tgaaaaccac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     3001 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgtgaac cacaggatca
     3061 cattccgtgg tcttctctgc tggcgctgcc gcagtcatat attgtcccgg gcggccgttt
     3121 tagcgaaacc tactattggg attcctattt caccatgctg gggctggcgg aaagtggtcg
     3181 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actacggcca
     3241 catccccaac ggcaaccgca cctattattt gagccgctcg caaccaccgg tttttgcgct
     3301 gatggtggag ttgtttgaag aagatggtgt acgcggcgcg cgccgctatc tcgaccacct
     3361 gaaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3421 tcgccatgtt gtgcggatgc cggacggctc gctgctcaac cgttactggg acgatcgcga
     3481 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3541 gcctaacgag gtgtaccgcg atttacgtgc gggggcggcc tccggttggg attactcttc
     3601 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3661 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3721 aggcgagaaa gagacagaag cactgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3781 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3841 gcaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggta tggcgaacca
     3901 tgaacaggcc gatcgtctgg caaacgccgt gcgcagccgg ttactgacac ctggcgggat
     3961 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     4021 acaatggatg gcaattcagg gatttaagat gtacggcgat gaccttctgg gtgatgaaat
     4081 cgcgcgcaac tggctgaaga cggtgaatca gttctatctg gaacagcaca aactgatcga
     4141 gaaataccat attgccgatg gtgttcctcg cgaaggcggc ggtggcgagt atccgttgca
     4201 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     4261 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     4321 ggagatattt aacatcattg ccgcctgggt gcgatttttg acttccagac ggcgatacag
     4381 tgattccaga tgcgctttta ccgttccggt actgatattc agcgccctgc cgatctcttt
     4441 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     4501 gagatcttta atgtcctttt ccggcgtcgt tcgccagtct ccaggcagaa acatcatccc
     4561 catcgccgca ctattgaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     4621 ggccagcaca ttaaaatgaa taacttcctg taaccaccgt ttgttgcaat ccgtcgccgt
     4681 aattaacacc ttaacctcag gaaattgcac cacggttttt tgcagcaacc tgtagcaaaa
     4741 ctcaccatcc tgatcgccat cgagcatcac taaggcttca gggtaacttt ccagcttttg
     4801 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gatgctgtaa
     4861 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4921 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4981 aaaaataaga attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag
     5041 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     5101 aacatttttg aggatttcct tatatcggta gttagtacgc atgcaattaa aaatgaaatt
     5161 ccgcgaccat aaacaaaaat aacaaacggc aatgagataa aaataagcac aaatagccaa
     5221 tacatcttct attcacttta aagggaatcg ctgaaaaata cgctctgttt aagggaattc
     5281 acctttctca taaagctatt cctccctttt cctgctgaga aatcgccaca ttcatcatga
     5341 caacattgtg aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     5401 ctactgtttc gttgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     5461 ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg
     5521 ccaaagggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     5581 ctccagcgca ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacgg
     5641 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     5701 agtatcagcg gtaaattacg catcgacatc ccgtcaggaa tcgcgaaaag cctgttactg
     5761 ccgcgcttgt cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat
     5821 gaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcatta
     5881 ccggaagatg gcgttatcgc ccgttctctc ggcaaactga ccatggtcaa ctgtgccagt
     5941 ccacactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     6001 atagtgcgtt atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     6061 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat
     6121 ctcgccgccg gtattgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     6181 gccctgcgtg ccgggcggct tattgaagtg ttgcctggct accgtgccga gccgctgtcc
     6241 ctttcactgg tttatccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag
     6301 tggctggctg gcgtaatgaa agagcacctg gactgaccga ctatactttt taagaatgac
     6361 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     6421 gatctggagc acgagccaat taagcagttg gataatagcg agaaaggcgg caaagttagc
     6481 caggcgctgg aaaccgtcac caccactgcc gagaaagtcc aacgccaacc ggttattgcg
     6541 cacctgattc gcgcgacaga acgctttaac gatcggctgg gtaaccagtt tggtgcggct
     6601 atcacctatt tctcattttt gtcgatgatc cccattttga tggtgtcgtt tgccgcaggg
     6661 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6721 aacattagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6781 cagcgtacga ctgtagggct tgtcggtctg gcagtggcgc tttattccgg catcaactgg
     6841 atgggtaacc tgcgtgaagc gattcgcgcc cagtcacgcg atgtctggga acgctcgccg
     6901 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcgct gattggtttg
     6961 ttgattgcgc tgattgtgac gctttctatc acctcggttg ccggttctgc gcagcaaatg
     7021 attattagcg cccttcacct gaacagcatt gagtggctga aaccgacatg gcggttgatt
     7081 ggcctggcga tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggttg
     7141 ccgcgccacc gtccgcgcaa aaaagccctg atccgcggaa catttctggc tgctattggt
     7201 tttgaagtga ttaaaatcgt gatgacctac accctgccat cgttgatgaa atcaccctct
     7261 ggcgcagcat ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg
     7321 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgccg
     7381 gggaaaacgc agccttaaaa taggccggat gcggcgtacg aatttattcg gcctggctcc
     7441 ccgtaggccg gataagatgc gccagcatcg catccggcta taatgcgcac ataacctctt
     7501 gaaactcttt cccagagcct ctttagccat ctattcagga gcaaacaatt tcattccaac
     7561 tcataacccc agcatataaa tccagttggt aacttttatt taacctaaaa ccagtttaat
     7621 ccaccattta taaaattatg tgaagcattt catagaagaa aaatcactgg cctaaacatt
     7681 atcccctttt tgcatgattt ttgacctttt ccgcgatttg ttacacattg aaatatcact
     7741 tttgctgtgc gtaatatggc tattcgttag ccaaaaaata agaaaagatt atgcaagcaa
     7801 cagccacaac actcgaccac gagcaagaat acacgccgat caactcgcgt aataaagtcc
     7861 ttgtcgcctc tctcattggc acggccattg agttcttcga cttttacatt tacgccactg
     7921 cggccgttat tgtgtttccg catatcttct tcccgcaggg cgatccaacg gcagcaacgc
     7981 tacagtcgct cgccaccttc gccatcgcct tcgtcgcgcg ccccattggc tctgccgttt
     8041 ttggtcattt tggcgatcgc gttgggcgta aagcgacgct ggtcgcctcg ttgctaacga
     8101 tggggatttc gaccgtggtg attggtctgc tgccaggcta tgccacgatt ggtattttcg
     8161 ccccgctgct gctggcgctg gctcgatttg gtcagggtct gggcttaggt ggtgaatggg
     8221 gcggcgcggc gctgctggcg actgaaaacg ccccaccgcg caaacgtgca ttgtacggct
     8281 ccttcccgca gctgggcgca ccgattggct tcttctttgc caacggcact ttcttgctgc
     8341 tttcctggct actgaccgac gagcagttta tgagctgggg ctggcgtgtg ccatttatct
     8401 tctcggcggt gctggtcatt atcggcctgt atgttcgcgt gtcgctgcat gagtcgccgg
     8461 tgtttgagaa agtcgctaaa gcgaaaaaac aggtgaagat cccgctgggt acgctgctga
     8521 ccaaacatgt tcgcgtaacc gtactgggta cgttcattat gctggcaacc tatacgctgt
     8581 tttacatcat gacggtctac tctatgacct ttagtaccgc cgccgcgcca gttgggcttg
     8641 gcctgccgcg taacgaagtg ttgtggatgc tgatgatgtc agttattggt tttggcgtga
     8701 tggtgcccgt cgccggatta ctggctgatg cctttggtcg ccgtaaaagc atggtaatca
     8761 tcaccacgct gatcatcctg ttcgcgctat tcgcctttaa ccctttgctc ggttctggca
     8821 acccgattct ggtttttgcc ttcctgctgc tggggttaag tctgatgggg ctgacctttg
     8881 gtccgatggg ggcgctgctg ccggagctgt tcccgacgga agtccgttat accggagcat
     8941 cgttctccta taacgtatcg tcgattctcg gggcttccgt tgcgccatat atcgcagcct
     9001 ggttgcagac taactacggg ttaggtgcgg tggggctata tctggcggcg atggctggct
     9061 tgacgttaat cgccctgttg ctgacccatg agacgcgaca tcagtcgttg taatcagagt
     9121 gaaaagcctg atgcgctacg cttatcaggc cagaatttcc atacaatata ttgaattcgc
     9181 aaggatttgt aagccggagg cgtttacgcc gcatccggca tgaacaaagc acactttgcc
     9241 aacaatctaa atctgtttaa ttgccggatg tcagacatcc ggcaattatc atcacttctt
     9301 catctgcgac aaaattgtcc tgcactgatt agcctcccct tcggaaggtg agatcagcgc
     9361 cagtaacgcc gccgccggtg ttaccagcgt tgccagtgcc gccgcaacgg ctccgcgagc
     9421 aatcaacggc ccggctttca ccccagcctg cggattttta aacgtcccac gcacatacag
     9481 cggcgaacgc agtgtgataa tccgaatccc tttactctcc ggatcaatag tcaaatccag
     9541 ctgttccgaa gcaaaacttg ccgtgccggt aacgttaatc aacgcgttct cagtatcgaa
     9601 agcaaaaacc tgcggacgcg ccacaccgtt ggcaatatcc agattcgctg ccgcgcagtt
     9661 cacccgcacc tcatcgtcac caaatatcgc accgacaatg tagttgccga cattcagccc
     9721 aacaatctcc atcaggttgc ggctcaccag cccgtcattc atcaacagtt tcaggttgcc
     9781 gttactgttg cctaaaagcg ccgccaccga gttaccgcta ccgcgcagtt ccgcgtcacc
     9841 gttcatttcc cccagcgtct tctgcatcag ttccacatcc ggcatcagtt ctttcagttt
     9901 cagccgacgg gcctgaatat ctgcccgccc ttgcatcggc tttttatcgc cttccagatg
     9961 aatattcgcc gcaatgctgc cgcccgccat gccaaatttc agcggttgca ggcgcaggtc
    10021 agcatttttg aggatgatat gagtagaaag atcgctaatc ggcaggctgc tgccatgctc
    10081 aatgcgccgc cctttgaagc gaacatcggc atccataacg tcccatttat cggtttcgaa
    10141 gcggtcataa ggcagcactt tgcccgcagg ctgaacgctt ttttcgccct tcttctgttc
    10201 agaccgtttc gacttttctg cccctttccc ggaatcaacg ccaatcaacg gtcccaggtc
    10261 cgccagccgc aattgccgcg actcgacatc accttccagt tttggtcgtg gcttgccggt
    10321 ggtatagatc agagaaccgt ggatatcgct atcgccaatg cgcccattaa aaccgcgata
    10381 atcaaagacc gacgattttt cagtgtcgat tttcgctacc agccgaccat ccgtttcaaa
    10441 cggcggtgta tcgggcagca gaacgcccgt cagttcatag agatcaccca gcgaatcgcc
    10501 agaaaattta agccgtaaat cgacaccccc catcttcatt gggtcattca caacgccatc
    10561 aaaagcaaca cgggtgttac cagagcggaa atcagcctgt accggaaacg gcgttccttc
    10621 gccacgcagc gccagcatac cgcctatttt tcccgtacca gtgagcggtt caccgttata
    10681 acgtccctgc gccttcaggc caaaaacgta atcgcccacc ttttctttat ccgctttacc
    10741 tttcgatccg gtaacttcgc tgaacggcag cggcttgcct aacggatcga caaaaatttc
    10801 cagatccgct ttgcttactt tgtcatcaat ggcgatccgc ccttgatcga aaagaatatt
    10861 atccagctga aacgaccacg ccgacggctt tgcattcgcg tctttgttgt catcgttggc
    10921 gagattaaac gtccagttat tgttcttttc ggagaggcga atcaggcgcg cgtcgggctt
    10981 ttcgagcttg atccacggca accagaccgt tttggtcagc agcgccagcg gggccagcgt
    11041 cgcttctact cgcggcaaat gcaccattgt gacttcggga atatccggtg gattgccaag
    11101 aatgatgtct tccgcgtgta catggggcca cggcacccag ctgcgccagc cagtttcttg
    11161 tttttgccgc tcccacacca cgcccagatc gccacggata gcgaacggac gattcaactc
    11221 cgcagagact ttctggttga tggtcggttt gagtcgattc cagtcaaaag ttgcaatcaa
    11281 aatgatcgcc acgacaatca acaacaagaa agcccctgaa atcgcagcgg ttattttgcc
    11341 tgccttgctc atcgttcgct tcctcatatt cttcctgtgc cagtcctaaa gatagtccag
    11401 ccaggcggaa aatgaggcag cttatagcgc cagaaccgcc gtattcagcg tttctatcgg
    11461 tgccgggcgt gaaagaaacc agccttgtgc ggcgaatgcg ggcgagttct gaacatcacg
    11521 ccactcttct ggtgtttcta cgccctcgac aatcaccccg cgacaatagc gattcattag
    11581 atgtaaaagc tgagaaaaga gtgtgcgtcc ttccggtgac tgacgcagca tcacaaacag
    11641 ttctcgcgcg attttgatgt agtcataacg cacttcactt agtgcagaga aatttgccat
    11701 cccggtacca aaatcatcca gccacagcgg gccaaattca cacatcgagg caaaggttga
    11761 atctttcggc aggcggatgt gttccaccag ttcgaaacgc agccagggaa gacgctcaat
    11821 ctggcgcagg atttttggtt gctgacgcag ggcgatgagc gtagggccat caatattgac
    11881 cgatgccagc aggccgtgct ctacaaagaa gtcggctttt tgcgccagca aatcaatctg
    11941 ctctttcaca acctccatcc gatggctaac ggtgatttca gtaaaatagc gatccggcgg
    12001 caggcgttgc gaagggttca agggatgcgt gaccaccgtt aatagctcca cggccattaa
    12061 ccgcccgcat gtttgataga tcggctgcca ggtgtaagca cgctcacact gcaaccaaaa
    12121 acgccgttcc tgcaagctct cgatgcttgc ttcagggttg cttattcgct ggataacctg
    12181 ccttatcatc aaagatgtcc tgtgtttaaa agtgatgctg cccggactcg acaaagatta
    12241 tcggcgcgca aaggcagaac tttaccgcct ggccggggta aatttcccca ctccacaatg
    12301 gaatctttca ggaatacgtt ctggctcaaa aaaataatgg aacactgttt taatatggtt
    12361 gaccagcaaa ccaccacagc gcaaactaac gctaattttt tacagatcag gttcacgact
    12421 atgtccaaaa agattgccgt gattggcgaa tgcatgattg agctttccga gaaaggcgcg
    12481 gacgttaagc gcggtttcgg cggcgatacc ctgaacactt ccgtctatat cgcccgtcag
    12541 gtcgatcctg cggcattaac cgttcattac gtaacggcgc tgggaacgga cagttttagc
    12601 cagcagatgc tggacgcctg gcgcggcgag aacgttgata cttccctgac ccaacggatg
    12661 gaaaaccgtc tgccgggcct ttactacatt gaaaccgaca gcaccggcga acgtacgttc
    12721 tactactggc gtaacgaagc cgccgccaaa ttctggctgg agagtgagca atccgcggcg
    12781 atttgcgaag agctggcgaa tttcgattat ctctacctga gcgggattag cctggcgatc
    12841 ttaagcccga ccagccgcga aaagctgctt tccctgctgc gcgaatgccg cgccaacggc
    12901 ggaaaagtga ttttcgacaa taactatcgt ccgcgcctgt gggccagcaa agaagagaca
    12961 cagcaggtgt accaacaaat gctggaatgc acggatatcg ccttcctgac gctggacgat
    13021 gaagacgcgc tgtggggtca acagccggtg gaagacgtca ttgcgcgcac ccataacgcg
    13081 ggcgtgaaag aagtggtggt gaaacgcggg gcggattctt gcctggtgtc cattgctggc
    13141 gaagggttag tggatgttcc ggcggtaaaa ctgccgaaag aaaaagtgat cgataccacc
    13201 gcagctggcg actctttcag tgccggttat ctggcggtac gtttgacggg cggaattgcg
    13261 gaagacgcag cgaaacgcgg gcacctgacc gcaagtaccg ttattcagta tcgcggcgcg
    13321 attatcccgc gtgaagcgat gccagcgtaa aagcagcata tgcaaaaggc tggatagagt
    13381 gttacccccc tatccagctc atccataaat acctgatggt tgtgctaact catcaccatc
    13441 aggcattact gtacaggtgg aatatccgtc acttcaggat gtacgtcatc cgtggcgaca
    13501 gatgtggcgg cagccgcagt cgatggggcc atgatttgat cccaggccgc ctgcaacgct
    13561 ttcatgttaa attccggctc gcctttcggc tgcagcagta ttaacgccat atcattcgac
    13621 aactgctgac gcagatcctg atttaacatc tccaccgtca ggctgttaag gaaatcctga
    13681 cgcagtttct gatattgctc cggcgcgata tcgacgacct gattttgcaa cgaacgcatc
    13741 cgctgcccca tcagaatatc ggtatcagct cgcgcatagg cggcaaacag tttctgcagc
    13801 tccagtttct tttgcgccac taacgcattg aactcttctt ccggcagacc tttatcgcga
    13861 accttcgcca gttcacgcgc caccagatta aggttgctgt tcagcttgtc gtttggtgat
    13921 tcgatgttga tggcacactg cgcacgcaga tacagcacac gacagtcaaa tccaagaccg
    13981 atgtctttac tgttactggc gcttaacgct tgctgaacat gccagaacag cgcctcacgg
    14041 gccaggtccg cacgccagta gcgcagcagt gcggctgatt cacgaatcgg ctgccacggc
    14101 gtatcccaca tgatagatag ccggtcctga cgcaccgcgt cggtcataat actcaccgct
    14161 tccgcacgca gcggagaaag cgtcggcacc ggagccggtg tttcacgttt gcctttcagt
    14221 tcgccaaacg ttttattgat ttggtcgacg accgagcgcg catccacgtt tcccaccacc
    14281 agcagcgtca ttgcatccgg ggtgtaccat ttctgataga aatctttaat cttttccgct
    14341 tcaacgggtt gtttcagcgg atcggcagga tcgtgaccta acaaggttga tcctttcaga
    14401 cgataacgcc accagccctc tttagtatcg gcaggccagg ttgccaccat gtcctgactt
    14461 tgcaacgcat ggttgatggt ttctggcgtg atagtcagtt tgccagtggc atttgccaga
    14521 taagagagcg cctctttcag caagtcgtta cggttattgg gcaaactcag attaaacagc
    14581 gtggtgtcat aagagacaat taccggcggc atcgggcgtt tagggtcgat cccctgctgc
    14641 cacaatgaac gcgcctgcgc tgcgtcaagg ccaccgcttt gcgttagcgc aatacgaggg
    14701 atggcgtgac tgtaaccgct ctgttgtgta ctttcggcga gcgaaccggt attaaccagc
    14761 aggcgaattt caacacgatc gctgggacgt tggggcgtag tcagcacttg ccactgtaaa
    14821 ccgttggaaa gcgtcccctg ttgccatgct ggatcaggct ggagcgcatc tgcctgcaca
    14881 tagccagcag tggccatcat cagcaaaccg cccgctaaaa gtcgaatttt tgtgccctgc
    14941 atgtgaaccc ctgatcaact atcctggtaa taaaaaactg cccgttgtcg gtcagtctaa
    15001 atgacgttaa aaacacttcg tgttagaccg caagaacatg aaaatgtcac ggaagaagtg
    15061 aaataaacct gaactcacct aggtggtaag ttcaggcaca ggggtcaatt atgcgcaaac
    15121 acccgcactc ggggaaggga gtgcgggcat aagtggtgag attaagagga taattcgtgc
    15181 gttttgccat ccggcgcacg attattcagc acatcgtcca gttttttgtg gtccagttct
    15241 ttcacccact tagcaacgac aatggtcgct acgccgttac cgactaggtt agtcagcgca
    15301 cgagcttctg acataaagcg gtcgataccg aggatcagcg ccagacccgc taccggcaaa
    15361 tggcccaccg cagagagcgt cgccgccagc acgataaaac cactacccgt tacccctgcc
    15421 gcccctttag aagaaagcag caacacgatt aacagcgtga tttggtggac gatatccatc
    15481 tgactgttag tggcctgggc gataaacacc gccgccattg tcaggtatat cgatgtgcca
    15541 tcaagattaa acgagtagcc tgtcgggatg accagcccca ccaccgattt gcggcagccg
    15601 agtttctcca tcttgtcgag catacgcggc agcgccgact cggaagatga agtccccagt
    15661 acaatcagca gttcttcacg gatgtaacgg ataaatttga agatactgaa accagtcgct
    15721 ttagcgattg aacccagtac cagcaccaca aacaggatac aggtgatgta gaagcagata
    15781 atcagctgcc ccagttgcac cagtgtgccg acgccgtatt taccgatggt aaacgccatt
    15841 gccccgaacg caccaatagg tgccagacgc atgatcatat tgatgatgcc gaaaatgacc
    15901 tgcgagaaac tttcgatcac gttaaaaatc agttggcctt tgctgcccag acggtggagc
    15961 gcaaaaccaa acagtacggc aaacagcagt acctgcagaa tgttaccgct ggcaaatgcg
    16021 ccaatgacgc tcgccgggat gacatccata atgaaggcga caatgccctg gtctttcgcc
    16081 tgatcggcgt aaaccgctac cgctttcgca tcaagcgttg ccggatcgac gttcattccg
    16141 gcaccaggct gcacgacgtt aacgatgata agaccaataa tcagcgcgat ggtactgaca
    16201 atttcaaagt aaagcagtgc gactgcgccg gtacgaccga ccgccttcat gctttccatg
    16261 cccgcaatgc ccgttacgac ggtacaaaag atgacaggag cgatgatcat cttaatgagc
    16321 ttaacgaagc cgtcgccaag cggtttcatt tgctcgccta tttcaggata gaaatggcca
    16381 aggagaatac caatggctat cgctgtcagg acctgaaagt aaaggctttt aaacagagag
    16441 gttttcatag ggtgtccttt agtaaaacca caggtcttgt aaggttatgg ggtacctgcg
    16501 gccttaaaat aacacccaga caacatcaca gaaatgtacc tggatcataa ttgaaacaaa
    16561 aaggttaaaa agtttgagct ggctcgcaca aaccagcact ttttaaagtt ttgtaatcag
    16621 tttggggtag ctacttttct tccaggtaac tctcttcgaa gatttcaata gggagtgggc
    16681 gagcaaaaag gaagccctgg gcaataccaa cgcccgcttt tgccagccag tcgcgttgtg
    16741 cttcagtctc cacgccttcg gcaatcattt gtaggttcag gctctgcgcc agcatgataa
    16801 ttgcagcaat catgctgcta tcttccggca aaccttcaac aaacattttg tcgattttca
    16861 gtacgtcgat tggcaacgat ttcatatgct gcagctgacg cagccctgcg tagcccatgc
    16921 cgaaatcatc cagcgccacc cgaactccgg cattgcgcag cggacggagg attgccaccg
    16981 cagcatgagg atcgtcaata cgtcggcttt ctgtcacttc cagaatcagt gttcccggct
    17041 gaatacgata gcgggttaac aattccagca tatccgccac catatttgga tgcatcagtt
    17101 gcagcgctga gaggtttacc gacaagggca gcataatgcc gcgctcttgc caggcagcaa
    17161 gcaatcgaca ggactcttcc agcacccagt gaccgacggt aaccatcagc ccacagcact
    17221 caatgcgatc gattaagcca tcgggcaggt cccaactgcc atccggttgc tggatacgca
    17281 gtaacacttc cgcactgacc agtttaccgc tggtcatctc gacctgtggc tgtaaccaaa
    17341 tcgcaaactg atgattttcc agtgcattaa ggatatcgct ctcttccgtc aaccgcttct
    17401 gggcggcttc catctgctgc ggatcaaaga actgaatctg atttttgcct ttatggcgag
    17461 cggtaaatgc cgcagaaata gcgcgactgt aaagctgttc ggcggtgaga tcgccgtaga
    17521 acatcgccac gccaatgcta cagtgcggac ggagttgaat acgttcaatc ggcaggcgct
    17581 cgctcatgat agtgagcact tgctgaccta atgtgatagc gtgccacggt tcctgtacac
    17641 cgttggcaat gacagcaaag tcataaccgc taatctgcgc gaggatcatg cgtggcgaca
    17701 gtaccgattt gagtttttcc accagcgtca gcagcagaat ttctcgttgc gcctctttca
    17761 gcacacccgc agtatcacgc agggtttcac aggtgatgat catcagcgcg gtagtttgtt
    17821 tacgcgccac cacctgctcc agcatctcca tcagcaaggc tttgttcggc aaatccgaca
    17881 ccgggaagcg catcgcattt tcgttctgtt cttcataatg gcgctgcagc aattgctggt
    17941 tgaggttgta actgcgcacc aacataccga tttcatcgtc ctgatgcaga cgcggtaatg
    18001 ccagttggtg accaacaagc tcctggggtg ggatggcgtt aagttcgcga gcaatattgc
    18061 gtaacggatg caaaatcagg cggttaatgc accagctaat cgccaccgtc aatattaacg
    18121 acaaaagtaa gtaaatggtc actaacgttg agagggtgct catcacgaac ttatacatgc
    18181 ggaaggaatc cgcctgtaac accagatagg caattggctg cgggtttgcc ggacgttcca
    18241 gcgagtaaac gcccagcgag atttgcaccg gtagctcaaa caggcgagta accattaccg
    18301 gcactggacg ctctggaata aaacttttac gcagcgcctg gaactggttg ggcagcacta
    18361 catcggcacg gctgaccacg ccggccggtt taatgctggc aagaatagct tccgcttcgg
    18421 gaatatcgcc tttcaaaatg gcagaagata agggttcgcg gacagagcga gcaatgcttt
    18481 ccagttgcgt agccgtgtta tagcgattct gctggaccag atggaacagc aaaacggtgc
    18541 aaaaaataaa aacgaacacc aggacaacgg ctgccaccat tgccatctgc ttgattgtta
    18601 acgagcgact tacgcgcaaa ttgtctcccc ccggaaatcc cagattgccg cccgagtata
    18661 cccgatcgca gcggcattaa gagaggcgct atctgaaaac ttaccagtcg gcgtaaggta
    18721 tcagcggctg cggcggtaaa tccatgtcac cctgccatcc ggcggcggaa taacgtacgt
    18781 agagcaggaa atggctgggt gcgtaatctt ttgcctgctg gatatcaatt gccgtgccga
    18841 caaaccagtt ggaagtaaca cgtcgttcaa gtaatgcccg cgccgtgtag ccaaagccct
    18901 gactgctgcc gccatcgttg gattgccgcg cagcttcttc ctgccagtcg gtcgggatca
    18961 gattcatcag cggataacgc ggcatggttt tggtgcgtga atgcgaccac gagccagacg
    19021 cccccagctc ccacgaccag ttttccgtgc gctcccgcca catcaccggt atggcaaacg
    19081 acaggtattc ctgcggactg tagtaaccgc cctgaccgag tgagtagcca ctcaggtctt
    19141 tgtcataatg ccagatcatg ttgttcaggc cgattgtgac gcggcgattg ttctggttga
    19201 tgaccttata gtaatagccc gtcatccagc gcacacgcca gttatcttcg acatttttac
    19261 cggttaactg atcaccgcta agcgatgccc agacgccgtt tgcttcacct ttatcgtagc
    19321 tcagacttag ccccacgccg tcggcacgca cgccgcccca ttttttcccg gtattgctcg
    19381 gagagtcttt ttgcccacca aaggccagca aagaactgga gataggccga cggtgggcgt
    19441 taacggtgta acccagcggc ccgatatcat cgctgtaact gatgccgccg accacatcca
    19501 ccacgttgaa gcccatcggc gtggtaccga tatcccagct ccagacgtca tttcgccagc
    19561 cgaccgccac gctggcaccg gaatccgact ggctgcggtt gccgctacag tcctgtaatg
    19621 tacaggtgcc ccagttgtca tcccatttgc catcggcatt agtggagaaa ctgccgacgt
    19681 tcatattgac gaaatcactg cgaaagaaca tccgcccgtc agaatagggc gcgtccacct
    19741 gcaacatagt ggtgtgcgct ttcagatcgg agtaaccacc ggtaccgctc gaaccccagt
    19801 aatcgtgctc gagagtgacg ttaagatcct gctggcgata gaggtccgcc gtatcgctgc
    19861 gcacgccgcg tttcagccag tcatctttct cgtcgttacg ggtcagccgg gtaaaggtgt
    19921 cgttatcctg cggacgcgtc gtggtcacac cggatgcaac catagcgtct ttgtaggttt
    19981 ccagcgcctg cgttggatcg cccgccagcg cttcaaactt cgcaccatca cgcagtacca
    20041 tcgcgctttc catcgacggt ggctgagatt ttgcctgcgg gatcaactta ttaaatgtct
    20101 gctgcgccgc tgcggtatcg ccaagctgcg cctgtgccag cgccacgcgc cgctgtgtgt
    20161 tcagcgaggc gttatcaatg gcaggcagtt tcgccagctg gctacgtgcc gccgctttgt
    20221 caccggcagc aatatccact tccgtcagac caagaatggc atcggcgtta gttggctccc
    20281 gcgtcagaac attctgatat gcagcgcggg cggcggtgta atcacgtcgc tgctgcgccc
    20341 agtcagccag cgtgaggtca atacgcgagg aaggcggttg ctggcgcagc atcgcttccg
    20401 cttctgcctc tttaccgttt tctcgcaggc gattagcggt ttccagcacc tgatcgtttt
    20461 gcagtcgatt aaccagctcc tgaatattgc tgttccactg cccgcgcggc aggctgttga
    20521 tatgcgccaa cgccgctctg tcctggtcgt gaccggagag gtacagcccg taagcgtaaa
    20581 cctgctccgg gtcgttcggc ttctgctgcg ccagattgcg cattaacgta tcggcctggc
    20641 tgcgttgtcc ggcctgccag agatcctgcg aaagtcggta agtaatccat acactgccgg
    20701 ggtccagcgc cagtcgttgc cgctgaagtg ctgccgcctg cgcccatttc ccctggtttt
    20761 ccagtgcctc tgcctgctgt gccagacggt cgttttgcag gctgcgttcg atatcatcaa
    20821 tgctgcgccg ctggctggca gagagcgagg cgatatacgc ttcggctttt tctggcgact
    20881 gctggcggta aatattcgcc agcccgcgca cggcgttagt gttgccgctg tccatacgca
    20941 aggtctgctg gtaatagcgt tcggcggcgg gataatcttt gcgcgccatc gccacatcgc
    21001 ccagcccaag cactgcatag ctgtcggtgt tatcgacatt acgtgcctgc tggaacagcc
    21061 gttctgcccg gtcaggatta ttggctttca gcgcggcatc gccctgctgg atcgccagcc
    21121 agtagcggtt tactttcagc agactgttcc atttgtcgtt attgctgttg tgcggatcca
    21181 gcgcgagggc tttttccaga ttcaccactg cattggcgcg atcgcctttc tgggaatacg
    21241 cctggcccag cgcccccaga gcctcactgt ctttcgggtt cgcccgcacc gcctgttgta
    21301 gttcgagaat ggctttaccc gccatgccag agtccaccgc cgctaaacct tgcgcacgag
    21361 cgcggaaagc agggtcggcc agctgttttt gctgttctgc cagttgcgat tgtgcagcag
    21421 ccacgctatc gccatcgcta aagatcgaga gatatttttt cagtgcctga acgctggcat
    21481 cgctgacggg catgtcttta atctgcccgt accagatttt agaggcccct tcgcgcccgg
    21541 cgttcgattt cgccatctgt tccaggacgg caaaaccttc gtcacggcga tcgctactaa
    21601 acagtaatag cgccagattg ttttgcaggc ccgtgttgcc cggcgtatcc gcattaatgc
    21661 gttttagctg attaatcgct tcgccacggc gggccggaat tttcgccacc gtactccagt
    21721 actcgacagc aatgtcacct tccggcggcg caccattgaa cagtttgttg taactcgcca
    21781 cagcttcttc cgcatgacca gtcgtcgcct gcaatcttgc ctgttgcagt gcctgacgac
    21841 catccgacgt ggaaagcagc atcgtagtcc gtgacgattt atacgcattt gaactcggcg
    21901 ctaactgcga cagccgatcg agctgttttt gtgcgccatc aatatcgccc tgacgtaaca
    21961 gagaacgaaa acgggcagca atcacatccg ggttattcgg atcaataagt tccagccgat
    22021 acaacgactg ttgcaccagg tcttcacgat gggtcgcttc gcctaaccga acttgctcca
    22081 gcaactgttg ctgagcggtt ggtgctgcct cgaccatcgg catgacggcc agaccgaggg
    22141 aaagcgtgaa tatgtttagt gtgaatttgc gcattcctgg ccccagtcag gtaataactc
    22201 accttttgtc gagaagcgga aacggtgttg atcccagcct tgtccaaaca gggtcagcac
    22261 atagttgtaa taggcatcgc tgccgggaaa gttatcggcc acgcgctggc gctgaacggc
    22321 ctgcgcatcg cggttttgta aaaagggcag catggcggca gaaaaaccga ccggtccttt
    22381 accctgcgct ttccccgtag ccacatccac tttttccggc ggataaccgt ttttctcagt
    22441 gaatgtcgcc atcggtttaa accggttgag catccgcgct ttttgcggat cgctgtcagg
    22501 catcatgcct acccacatgt aaacgcggat agcgtcgtag ctgctgatca atgttttttc
    22561 ggcttttagc tgccagcctt tgtctttctc atagcgcacc cagtctggcg aaaagccttt
    22621 cggggcggtt tccagcaata aacgttgatt ggtttcgcgc agcgtggtcc acggcgcgcc
    22681 aaagcgggtg aaatactgcg ccagcgtcgg cggcaggtag ctggggttaa aacgccagct
    22741 gttatcctca gcaaaaccca ctttgcccgg taacaacatg gagcccagcc caggcaccgt
    22801 caccacttcc tcccgcgcga tacgttttag caatgcgctg ccgatgtcgg tataacgctg
    22861 ctctttccac aaacgccccg cctccagcaa cgcccaggcc atccagacat caccatcgga
    22921 ggccgaattg ctgtccagca cttcccactt actgttctct ttcttgcccc acagccaggc
    22981 gggcaaatgt tctgttaaag aaccctgagc gagattgttc tgcgtccagt cgagaagatt
    23041 atcgaaagct gcacggtcgt tagccgccag ggcaaagaac atgccgtaac tttgcccttc
    23101 ggaggtggtg attttgcgcg cgtcgctggg gtcgatgacg cgcccttcct gactgatgta
    23161 atccttttta aactgctccc aggcaggcca ggtacaggct gcctgaacac taaaggcagc
    23221 cagcagcagc atcgtcacga gtccactacg caacacattc atcttcaatt actcgttatc
    23281 cgggttaaga cgacgacgac taataatacg cagcagacgc cacagtaccc acgccagcaa
    23341 tatcacactg atagccgcca gcaccgccag cagaatcgga tggtttgcca gcgcatacca
    23401 caagcgctcg aaccacggca gatgacctac gtaataaaca tcgccaacac gtaggctgtt
    23461 gataccggac tcgcggatca ccgcgaccga accgaacatg gtggcgcgtt tgccgctatc
    23521 gttcaccgca tcgttaagca tttcataacc gcgtgggcta tctgccagca gcgcaatcac
    23581 gctgcgctgg tcgttatacg gcgactggaa gccaatcacc gccgccatcg caccggaaga
    23641 ggtcagcgtt gactgagttt ctgccgcgcg atcgctctcg tccggcacaa taccggggaa
    23701 cggggtctgg cgcatcggcg ttttcaccca gctttcggta gcctgcacca acaggtcgat
    23761 ctgcttatcg tctttcagtt tgtccgggat accaccgacg atcatgatgt cggcatcttt
    23821 gccctgaatg gtgctgccat catcggtcac cgtcaggtta atcgccggga agcccgtctg
    23881 cgcaccgata aagccaacgg tattcagcaa ggtttccatc tgtgcttcgt ttggcgtttt
    23941 cggcatcacg gtgatggttt gcgacaaatc cgccatccgg ctgaacggga agcccgcgtt
    24001 agcaaaggcg cgtagatccg gcatcgggat gaagtggtaa tacttcgaga agtcgatggt
    24061 ggagtcgtca ccaatcacca catgattctg caccggctgg aaggtaatac agttatccac
    24121 cgaaccgccc ggcatcgggt tcatatactc aaagtcgaac cgcaactggt tggtcgcgcc
    24181 cagtttcagc gccggaatag agacatccgt tttgccatcc agcaaacctt gtaataccgg
    24241 aatccgcagc agcaggcggt tcgcctcctg tttgctgctc aggttgaagg attgcaggaa
    24301 ctggttattc aggctgatat ccatccgcga actgtctttc accggcggca tggtgtagcg
    24361 gtaattaata tccatatcaa tgccggtact gcgcatcagg tagagatccg gcggcaggtt
    24421 tagcgaaacg ttaatcgctg ctggctcaag accgctggat tgtaattgtt cttcataggt
    24481 tttcagttca ccaaaggtga ccggacgatc ggtacgcacc cagttcggcg catcgtacgg
    24541 cttacgtggt agcagcggtt tcacttcatt cactaccaca ctttcaccac ggaacagaat
    24601 gttaccctga gcgatacctt tcgctgcctg caacaagtct ttgtcgtcac gaccaaacac
    24661 caccagcagt ttgacgtaag gattctgcgg atggttaatc atctcaatca ccggggcttt
    24721 taccgccgga tgatcgcgca ggaagtccgg gcgtttgtca ttggtggcaa agacaatggc
    24781 attgcgatcc ggcagttggt tatagagcac cgggaagttc tgcccacgcc agccagaacg
    24841 cgaaccaaac cacgaggcga caatggcaga tgcttgttgc agctcaacat ccggcgcacc
    24901 cgcaaagacc atcggcaagg tgttggtgcg gttatcacgc gggtcaaaga atggcaccgg
    24961 gaagtgtgac aggtcattct tcacattcag ggtctgatag gtcagatcca gtccactgct
    25021 ccgcccaaca tccagccaaa gcgtggtgct ggccgggttt tcgcacacgt cctgataatg
    25081 gccgacaaac tccagccgca cacggttgaa atcagtaata aacagcgggt taatgggcat
    25141 ttgtgccagc gtttttttac ccaactgttc tttggtcact ggcagcacgc ccatcagttc
    25201 atcattgaga taaaccttta actgcgactg gacaggcagt aatgatggcg atggcgtgta
    25261 ttcgaggttg agcatcgcct tcgtcaccac ttcatcgctg cgcataccaa actcaatgct
    25321 gccgttcggg ttaatgccac gtagcaccat gctgcccggc ggcggcgcaa tttgcgcaaa
    25381 ggtcagcttc acatcacggg aaggaccgtt ctgcgccacg actggcgcat ctgcgccctg
    25441 cacgcccggc atcacttgcc ccacctgcgg attttgctcc gcctgggcgg ctacagctgg
    25501 ctcagcattg atcagtggtt gcgttgctgg cgtcgcctgc gtcatgaaag aggggaacgc
    25561 actcatcccc agagccactg cacaaatcca gaatattttt cttttcatcg cgttatcatc
    25621 attgttgagc caaagcctga tccgatggtt gtgccgtttc gctccgctcc gggcggcgcg
    25681 gaataaacga tacaacccag gaaaccagag aagtcagcac acggaatatg cccttcaccg
    25741 aagaaggcgc aaattccgcc agatggcggt agccacggaa gccgagcttc agaatatcca
    25801 gcagactttc cagcggctta tcttccgggt agctgtcctg ccagagcgcc catgtatccg
    25861 cacgggcaaa cgtacactgc acaaaatcga tatgttgctg ggtagtgagc ggcattaatt
    25921 tcagcccaac ttcattaccc atcacgcgcg ccacctgagc cgggaagacg tattcctgct
    25981 gaccgcgttt aagcaacaga ttcactttct gcccttccag aatctgcgcc tgaccgttga
    26041 tcttgatccc caaaccaccg tcggagaaat cctgaacggt acacgagaag aggtgaccat
    26101 cttcgcgggc aattgctgcg ggcatcgtca tctccacgcg gtgcgatcgg cgtacctgtt
    26161 tgctttctac cgatactgcg actgcgccgc cgagaataat caggttgtag aatacccaca
    26221 ccatactgac gaccacggtg agcatctcgg ttggcggacc atagaagtag cgccagatcc
    26281 caaccgctac gcccaccagg ttgagcagaa caaggaagat gtagggccgc gagatcaccc
    26341 agtcgacgta ctcttcttcc actagtccac ctttggcggt gacgttaaat ttgcctttgt
    26401 gcgggttaat cagcgccacc agcgtcggtg gtgcgatata ccacgccagc accgtttcgt
    26461 agatttcact ccagaaagag tggcgatatt tgccctggat cttcgagtta gtcaggctgg
    26521 catggatcat atgcggtagc acaaacaggg cgatcatcaa cgctggcgcg tagatgatgt
    26581 aggcatgaaa cagcaggaac gccagcggcg cagtcaggaa gatcagccgt ggaatgcccg
    26641 acaagaagtg gaacatggcg ttgacatagc acagtcgctg agcaaacttc agccctttac
    26701 cggtgagcgg gttatcgaga cggaagattt gcaccatccc gcgcgcccag cgaatacgct
    26761 gaccgatatg cgccgagaga ctttcggtcg ccagccccgc tgcctgcgga atacgcatat
    26821 acgcggaggt gtagccacga cggtgcagac gcagagaagt gtgcgcatct tcggttaccg
    26881 tttcgacagc aataccgcca atctcatcca gcggcttacg gcgaatcacc gcacatgagc
    26941 cgcagaagaa agtggcatcc cacatatcat tgccgtcctg caccagacca tagaacagcg
    27001 tgccttcgtt cggtgtttta cggaaacgcc ccaggttgcg ttcaaacgga tccggcgaga
    27061 agaagtggtg cggcgtctgc atcatcgcca gctgtttctc tttcaggaac cagcccatgg
    27121 tcatttgcag gaacgaacgt gtgggaacgt ggtcgcagtc gaatatcgac acgaactcgc
    27181 ctttggcata tttcagcgca ttgttgatgt tgcccgcttt cgcatgttca tgagtggtac
    27241 gggcgatata tttcaccccc acgttttgcg caaactggcg aaactcttcc ctgccgccgt
    27301 catcgaggat ccagatgttc agcttgtctt tcggccagtc gatacccagc gaagcgtaaa
    27361 tggtattttt caccacgttg agatcttcgt tgtaagtcgg gacaaagata tccaccgacg
    27421 gccacagcga catatctttc ggcaatggca ccggctgacg attcagaggc catacgacct
    27481 ggaaatagcc gagcaccagc acaatccacg cgtacgtttc ggcgaacagc agaataagcc
    27541 cgcacaccag gctgaccgga tcgtcccagt ttaacgtcga ggtgtaacgc caccagatat
    27601 aacggcaaga aacggtcagc gacagcacaa tcagcatcag cgccgagaag cgccccggca
    27661 tccgccgtac gatcagcgct accccccaca gcagcatcag gaagataaac tgcgctagcg
    27721 ggttaaacgg ctgagtaacg cagattaacg ccagaatcag cgagaaggtg acgatgatac
    27781 caaggatcaa ccgccgcgcc ccggcactca aatgaccgag ctcttttttc tcatcaagat
    27841 gctgtgtttt atggctaacg cgctcaggca gctcgttcat ccattgatgg taacgtccac
    27901 gaatattttg tagacctgaa aatgcccgcc tgcgcggttt cggcgtttct ttgcgcgatg
    27961 caccgatcag taaccagcat gtttgaataa gataacggac cgggtccagc ggacgcggac
    28021 gcgaggcgtt gatatgcgga tacaggtttt tatgttctgc gcgaatacgc tgccagcgcg
    28081 ggtgctccag cggaataaaa atccaggcca ggatcatcca gaaacagccg agcgtcgcgc
    28141 tgaaagccga cgcgccgtga cgacgataat cgcgataacg cccgataagc cgtgcgttga
    28201 ccggcgggat aagcaaccac cgggtcagga tactcatgat gcactcccga ctggcgtttt
    28261 cagcccggaa tagtttaaca ggcaccagtt agccagcgtc agtatttctt cagccgccag
    28321 cgcatcactg cgaaattcac cgactggttg cttagctgcc agacattcag ccatcgcttc
    28381 atcacgatga atgagcatcg gcagcaatcg gcgctggctt tgcaaccaaa gctggtaaat
    28441 atcgtcctga acctggctgc caatacggaa gtcattaatc aaaatatgtg caccatccgg
    28501 cagcgcttgc tgatgcagtc ggatatggca gttggcatcc acattgacga ttgccagcga
    28561 gtgatcgcac aaactcagca gctggtgggt tatctgcgag gcatcacgcg gtaagtcaat
    28621 taaaatccac tggtaacgcc cgctggcttt tagttgctgt aagccggagc aaatatcgct
    28681 cagccgggtt tgccagtgct gtggattttc ttgttcttca atggataact gaccaaaagg
    28741 cagcaaatcg agctgcgagg tatagcgtaa cccggcgtca caccagtcct ggccatccag
    28801 catagctctg gcccaaccct gacggtgggt aaaatcaacg ttaaatgaca ggcgcaacaa
    28861 gttgtccggg caggcatcga ccaccaggac attttctccc aacatttgta atgaccaggc
    28921 taatgcggcg gtgatggttg ttgtccccac gcctccccga accccctgca atcccagtac
    28981 ggccattctc caatccccta cttttgttgc gcaaactctg ccagcaacgg ccagcgtttt
    29041 aatgccgcgg ccaactgttc gcgttgggaa atatcggcat aatcaatatc aggcagtgaa
    29101 aatgcctgct ttaacgccaa aatatcattc tggaagatat agcctatcgc gggatcaggc
    29161 agagtatctg gttcattgtt attcattttt cttgatccct atgaaataac cgtaccgttc
    29221 atgagacaat tctaactgtg agcatttcgt cggtttattg tttacggcga gacgggaatg
    29281 tgctcacttt ttgactttcc gttacgcaac agaaaaagtt aaaaacgaaa agactaaaat
    29341 cacatgtctt gtgacctgct aaattgattc acatgctaaa tctgataagt tttaatttca
    29401 atggtaggtt tatttcttag ctttcgctag taaactgata atcagacaaa atgttgacat
    29461 gagggacatt gtggaccctg tattctctat cggtatctca tcattatggg atgagctgcg
    29521 acatatgcca gcaggcggcg tctggtggtt taacgtcgat cgccatgaag atgctatcag
    29581 tctggcgaat caaacaattg catcccaggc tgaaaccgca cacgtcgcgg tcattagcat
    29641 ggacagcgat ccagcgaaaa tctttcaatt agatgattct caagggccgg gaaaaataac
    29701 attattttca atgctaaatc atgaaaaagg tctatactat ttggcccgtg atttgcagtg
    29761 ttctattgat ccccataatt acctttttat tcttgtttgc gcaaataacg catggcaaaa
    29821 cattcctgcc gagcggcttc gctcatggtt ggataaaatg aataaatgga gccggctaaa
    29881 ccattgttcg cttttggtaa ttaattccgg aaataataac gataaacaat tttcattgtt
    29941 acttgaggaa taccgttcac tttttggtct tgccagtttg cgttttcagg gcgaccaaca
    30001 tttgctggat attgctttct ggtgcaacga aaaaggggtc agcgcccgtc agcagcttag
    30061 cgttcagcaa caaaatggtt gctggacatt agttcaacac caggaggcgg aaatccaacc
    30121 acgcagcgac gaaaaacgca ttctgagtaa tgttgctgta ctggaaggtg cgccgccgct
    30181 atcggaacac tggcaactgt tcaacaataa cgaagtcctg ttcaatgaag cccgtaccgc
    30241 tcaggcggcg acggtggtct tttctttaca gcaaaatgcg caaatcgagc cactggcccg
    30301 cagcattcat actctgcgtc gccagcgcgg tagtgcgatg aaaatcctcg tacgggaaaa
    30361 taccgccagc ctgcgcgcca ccgatgaacg tttattattg gcctgcggtg caaatatggt
    30421 tatcccgtgg aatgcgccac tctcccgctg tctgacgatg atcgaaagcg tgcaagggca
    30481 gaagtttagt cgctatgtgc cggaagatat cactaccttg ctgtcaatga cccagccgct
    30541 caaactgcgt ggtttccaga agtgggatgt gttctgtaat gccgtcaaca acatgatgaa
    30601 taaccctcta ttacctgccc acggtaaagg cgttctggtt gccctacgtc cggtaccggg
    30661 tatccgcgtt gagcaagccc tgacgctatg tcgccctaac cgtaccggcg atatcatgac
    30721 cattggcggt aatcggctgg tgctgtttct ctcattctgt cggattaacg atctggatac
    30781 cgcgttgaat catattttcc cattgcctac tggcgacatt ttctcaaacc gaatggtctg
    30841 gtttgaagat gatcaaatca gtgccgagct ggtgcagatg cgcctgcttg ccccagaaca
    30901 atggggcatg ccgctgcctt tagcgcaaag ttctaaaccg gtcatcaatg ccgagcacga
    30961 tggtcgccac tggcgacgaa taccagaacc aatgcgactg ttagatgatg ctgtggagcg
    31021 ctcatcatga tgaccatcag cgatatcatt gaaattattg tcgtttgcgc actgatattt
    31081 ttcccgctgg gctatctggc gcggcactct ttgcgacgca ttcgcgacac cttacgtttg
    31141 ttctttgcta aacctcgtta tgttaaaccg gccgggacgt tacgccgcac ggaaaaagcc
    31201 agggcaacca aaaaatgact caatttacgc aaaataccgc catgccttct tccctctggc
    31261 aatactggcg cggcctttcc ggctggaact tctattttct ggttaagttc ggcctgttgt
    31321 gggcgggata tcttaacttc catccgctcc tcaatctggt gtttgccgcg tttctgctga
    31381 tgcccattcc gcgctacagc ctgcatcgct tgcgccactg gattgccctg ccgatcggct
    31441 ttgctttgtt ctggcatgac acctggttgc ctggcccgga aagcataatg agccagggtt
    31501 cgcaggtggc ggggttcagt accgattatt taatcgatct tgtcacacgc tttattaact
    31561 ggcagatgat tggggccatt tttgttttat tcgtggcctg gttattcctg tcacaatgga
    31621 ttcgcattac cgtttttgtg gttgccattc tgctatggct gaacgtactt accctggcgg
    31681 gaccaagttt ctccttgtgg ccagccggtc aaccgacgac cactgtaaca acgacgggtg
    31741 gtaacgcagc ggcaaccgtt gcggcgacgg gtggcgcacc ggtagtgggt gatatacccg
    31801 cacaaactgc accgccaaca acagcaaacc ttaacgcctg gctgaataat ttctataacg
    31861 cggaggcgaa acgtaaatcg atcttcccgt cttcgctgcc cgctgatgct cagccatttg
    31921 aactactggt aattaatatc tgttcgcttt cctggtcgga tattgaagcc gccggattga
    31981 tgtcgcatcc actgtggtcg catttcgata ttgagttcaa gaactttaac tccgccacct
    32041 cctacagtgg cccggcggcg atccgtttac tgcgcgccag ctgcgggcag acttcgcaca
    32101 ctaatctgta tcaaccggca aataacgact gctatctgtt tgataacctg tcgaaactgg
    32161 gctttaccca gcacctgatg atgggacata acggccagtt cggtggtttt ttgaaggaag
    32221 ttcgcgaaaa tggcggcatg cagagcgaat tgatggatca aacaaatctg ccggttattt
    32281 tgctgggctt tgatggttcg ccggtttatg acgataccgc cgtgcttaac cgttggctgg
    32341 acgttaccga aaaagataaa aacagccgta gtgccacgtt ctacaacacg cttccactgc
    32401 atgacggcaa ccattatccg ggcgtcagca aaacagcgga ttacaaagcg cgggcgcaaa
    32461 aattctttga tgaactggac accttcttta ctgaactgga gaaatcgggt cgtaaagtga
    32521 tggtggtagt ggtgccggaa cacggcggcg cgctgaaggg cgacagaatg cagatatctg
    32581 gtctacgtga tatccctagc ccgtctatca cagacgtccc cgttggggtg aaattcttcg
    32641 gcatgaaggc accacatcag ggggctccga ttgtcatcga ccaaccgagc agcttcctgg
    32701 ctatctccga tctggtggtt cgcgttcttg atggcaagat tttcaccgaa gacaatgttg
    32761 actggaaaaa actcaccagt gggttgccac aaacagcacc ggtctccgag aactcaaatg
    32821 cagtagttat tcaataccag gataaaccgt acgttcgcct gaacggcggc gactgggtgc
    32881 cttacccgca ataagaacga aaaaggccgc agagtttcac ccctgcggcc tgatccgggc
    32941 gcaaattgcc attacggcag cctgacgccc gcatgacacg ttacttccgc ttattcagcc
    33001 agttcacgat catactggca agaatgccag caatgaccgg agccgctaaa tcatgccaga
    33061 aggccatgcc cagttctgcg agcgtcatat agccgcctgt gttgtaatga caacgtttca
    33121 cggctattct tgagttgttc aggttcaaga ttagcccccg tgatgttgtc aggtgcatac
    33181 ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat taagcccccg
    33241 caacattgcg cctcaccgga acctttccgg cttgccgctg attttacgat tgctttttgc
    33301 cgtcagcacc cagcaatctt cctcattgcg tgatgccttc cagcttaatt tctttatttg
    33361 caatagttgc agctcatatc cgggctgact ccagactagt agtgaaatgc gcaattttgt
    33421 agaattgcgc ctcctcgcga cctccccagt gaatctgttg cctcattttt tacatactgc
    33481 gtattcgact tctccacctg ttgcgcaaga gaaactgggt ttattcattt ttgcgaggcc
    33541 gacttctttc tggacaggac tttatgcagc acaacacact accgaaacac gatcagaaat
    33601 tgccgtttac acgctacgac ttcggctggg ttttattatg cataggcatg gcgattggtg
    33661 ccggaaccgt gctgatgcca gtacaaattg gcttgaaggg aatttgggta tttattaccg
    33721 cagcgatcat tgcttatcct gccacctggg tagtacagga catttattta aaaacccttt
    33781 ctgaaagcga ttcctgtaat gactacaccg atattatcag tcattacctg gggaagaact
    33841 ggggaatttt cctcggggtt atctactttt tgatgattat ccacgggatt tttatctact
    33901 ctctctccgt ggttttcgac agcgcctcgt acctgaaaac cttcggttta accgatgccg
    33961 atctttctca atctctattt tataaagtcg ccattttcgc cgtactggtg gcgattgcgt
    34021 ctggtggtga acgattactg tttaagattt ccggaccaat ggtggtggtc aaagtgggga
    34081 ttattgtcgt gttcggtttt gcgatgatcc cgcactggaa tttcgccaat ataaccgcct
    34141 tcccgcaagc ctccgacttt ttccgcgatg tcctgcttac tattccattt tgcttctttt
    34201 ccgcggtatt tattcaggta cttaacccaa tgaatattgc ctatcgtaaa cgggaagcgg
    34261 ataaagtact ggcaacccgg ctcgcgctgc gtacccaccg gattagctat atcacgctca
    34321 ttgcggtgat cttgtttttt gccttttcgt ttaccttctc aattagccac gaagaagccg
    34381 tttctgcctt tgaacaaaat atctcggcac tggcactggc cgcgcaggtg atccctgggc
    34441 atatcattca tatcacctct acggtgctga atatctttgc cgtactgact gcattctttg
    34501 gcatttatct cggtttccac gaggccatta aaggcattat tctcaatctg ttaagccgaa
    34561 ttattgatac caggaaaatt aactcacgca tgctgactct ggcgatctgc gcttttatcg
    34621 tcattacgtt gacgatttgg gtttcgtttc gtgtatcggt actggtgttc tttcagttgg
    34681 gaagcccgtt atatggcatt gtgtcgtgcc tcattccgtt tttcctgatc tataaagtct
    34741 cacaactgga aaaacttcgc gggtttaaag cctggatgat tctgctgtac ggcattttgt
    34801 tatgcttgtc gccactgttg aagttgattg agtaaaccgg agcgcatggc cccggttttg
    34861 tgagttaacg ctgcggattt tcatcctgat caacggcaaa acaggctacc agttgaccgc
    34921 cgtagtcttt tagctgcggc tgcaactgga tgcaggggcc gaagcgccga cgacagcggg
    34981 cgttgaaggc gcagcctggc ggtggattca gcgggcttgg cagttcaccg gtaagtttga
    35041 tgcgttcgcg acgatcgtcc gggttcaggc gtggtgtcgc ggagagcagc gcctgagtgt
    35101 acgggtggcg cgggttattg aagatttggt ccttcgtccc cttctccacg cagcggccca
    35161 ggtacatcac catcacttca tcagcaatgt gctccaccac cgacagatcg tgcgagataa
    35221 agacataaga tagccccagt tcctgctgca aatccatcat cagattcagc acctgcgcac
    35281 gcactgagac gtccagcgcg gaaaccggct catcggcaat caccacatcc gggtcgagca
    35341 tcagacctcg ggcaatggcg atacgctgac gctgaccgcc ggagaacata tgcggatagc
    35401 ggtcgtaatg ctcggttttc aggccgactt tcgccatcat cgacagggct ttttcctgac
    35461 gctgttcttt gcttaagctg gtgttgatca gcagcggctc ttcgagaatc tgcccaactt
    35521 ttttacgcgg attcagcgaa ccgtaagggt tctggaagac aatctggatt ttctgccgac
    35581 gtagcttctg cgcctgcgga tcgtgcttga gcagatcctg cccctggtaa tacagttcgc
    35641 cgccggtggg cgtttcaatc atcgtcagca accgacctag ggtcgattta ccgcagccag
    35701 attcacctac tactgccagc gttttgccac gttcaaggtt aaacgaaacg ccatccagcg
    35761 ccttcaccag acgttccggc gcgaacatgc ctttcttcac cggataatgt tttttcaggt
    35821 cgatagcctg caacagcggt tgttgcgggg tggcctcatg cgtactcata gtgtcggcct
    35881 cccggcatca tcaagtgggt aatggcattt ggactgacgc ccgtcagcga gcatattcag
    35941 cgccggttct tcagcacggc atttgtcagt ggcgtagggg cagcgcgggt taagcaggca
    36001 gccgttcggg cggtcgtact tgccgggaac gacacctggc aacgacgcca gacgttcttt
    36061 gtcctgagca aattccggca gcgcacgcag caatgcctga gtatacgggt gacgcggcgc
    36121 atggaagata gcgtgcgcat cgcccgtttc tactacctgg cctgcataca tcacgataat
    36181 tttatgtgcc gcttccgcca ccagcgccag gtcatgggta attaacacca gcgccatgtt
    36241 ctctttctgc tgtagctcca gcagcagttc gataatttgc gcctgaatgg tcacgtccag
    36301 cgcagtggtt ggttcatcgg caatcagcag ttttggccga caggcaatcg ccatggcgat
    36361 catcacgcgc tggctcatgc cgccggaaag ctgatgcggg taaacatcca gacgcgatgc
    36421 cggatcggga ataccgacca gattcagcag gtcaatcgct cgctgacggc gggtactttt
    36481 gttgccgcct tgatgcacct taatcgcttc cataatctgg aaacccacgg tgtagcacgg
    36541 gttaaggctg gtcatcgggt cctggaagat cattgccact tcggcaccca ccaggttgcg
    36601 gcgctctttt tctgagatac gctgcaaatc ctgaccgtta aactccagct tttccgccat
    36661 tacgcggccc ggataatcaa tcagccccat aattgccagc gaactgaccg atttaccaga
    36721 gccggactca cccacaatcc cgaccacttc accctgtttt acgctgtagc tgatgcggtc
    36781 tacggcgcgg aacggtgcgc tttcgtcgcc gaaatgcacc gataatttat ctacatttaa
    36841 taacgccatc tcgaacctct tactgcttca gtttgggatc gagcgcgtca cgcagaccgt
    36901 cacccatcag gttaaatgcc agcaccgtca gcaggatcgc cagacccggg aaggtcacga
    36961 cccaccaggc gctttgcgcg aactgcaaca catcagagag catggtgccc cactccggcg
    37021 ttggcggctg cgcgcccatg ccgaggaaac caagagcagc catatcgaga atggcgttag
    37081 agaaaccgag cgacgcctga acaatcagcg gcgcaaggca gttcgggaag atgttaataa
    37141 acatctggcg cattgccccg gcacccgcca cgcgagacgc ggtgacgtaa tcgcggttaa
    37201 cttccaccag cacggcggcg cgggttaagc gcacataatg cggcagcgca acgaaggtta
    37261 gcgccagcgc ggcgttacca atcgacgggc cgaaaatcgc caccagcacc agtgccagca
    37321 gtagacttgg cagcgccagc atgatatcga ccacgcgcat gatgatgtta tcgaccagac
    37381 cgccaaagta accggcgatc aggccgagaa taacgcccat aatcagcgat aacaccacta
    37441 ccagacagcc aaccagcagc gacaagcgcg cgccgtacat cagccgagac agcacatcac
    37501 gccccacgtc atcagtgccc agcaagtgcg ccatgctgcc gccttcctgc caggctggcg
    37561 gggcgagcag tgcatcgcgg aactgttccg ctgggttata gggtgcaatc cagttggcaa
    37621 agatcgcgat aaacagcacg atgacgacgt aaaccagccc gacaaccgcg cctttgttgc
    37681 gtttaaaata gtgccagaac tcctgtaacg gggtcatcgg caccggtgcg ctaatcactt
    37741 tattttcagt aacctgtgac atgatgtccc cttacttctt atgacgaata cgcgggttca
    37801 ccacgccgta cagcagatcg accagcaggt tgacgaggat aatcatcgtc gccaccagca
    37861 atacgccgcc ctgcactacc ggataatcgc ggcgttgcag tgcgtcaatc aaccagcgcc
    37921 ccagaccggg ccacgagaag atggtttcag tcagaatcgc ccccgccagc aatgttccca
    37981 cctgcaggcc gataacggtc accaccggca gcatcgcgtt acgcagcgca tggacgataa
    38041 tcacccgcat ccgggtaaga cctttggcac gcgcggtgcg gatataatct tcgcccagca
    38101 cttccagcat cgaggagcgc gtcatacgca caatgaccgc cagcggaata gtacccagca
    38161 caatggcggg caggatcata tgggcgacgg catcgataaa gttaccgtct tcaccccaga
    38221 tggcggtgtc gatcagcata aaaccggtta acggattgga atcatcgagg aacaccatgt
    38281 cgctcacgcg accggagaca ggcgtcaggt tccagtgcac cgaaaccagc atgatcagca
    38341 tcatgcccca ccagaaaatt ggcatcgagt agccggtcag cgccaggcca accgctgtgt
    38401 gatcgaaaat ggaaccacgt ttaaccgcag ccagcacacc aaccggaatc ccgactgcgg
    38461 tcgcaaaaat catcgcgcag acgccaagtt ccagcgtggc ctgaaagcgc ggcacgaact
    38521 cttcccatac cggaattcgg cttttcatcg aaatgcctaa atcaccgtgc attacgcccc
    38581 aaatgtaatg gagatactgc tgccacatcg gtttatctaa gccgagttca gccagcaatt
    38641 gcgcgtgacg ctctggggag atcccacgtt cgcccgccat gatcatcacc ggatcgcccg
    38701 ggatcatgtg gacaaaggca aatgtgagaa gggtaatacc gataaacgtg gggatgacga
    38761 gtcccaaacg tcggagaata aactgcaaca taacccggat tctctatagt gacccgcagc
    38821 ctggaactgc gtgtctgtat tgctcacaaa tcaattcccg cccttgtggg acagggaatg
    38881 aagtattgct gccggatggc gctgcacgca gaggcacagc accaccgggt atggctttta
    38941 attattcgat agagacgttt tcgaagtgat gtttgcctaa tggatcaacc acatagcctt
    39001 taacttcttt gcgtaccggt tcaaacacgg tggagtgagc gatgatcagt gccggagcct
    39061 gatcgtgcat cacgacctgt gcttgtttgt acagttcaac acgtttattg tggtcgtcgg
    39121 tagcacgcgc cggttgaatc agatcttcaa acggtttgta gcaccatttt gagtagttgg
    39181 agccttgttc agaggcggcg cagctgaaca gggtggcgaa gaagttatcc ggatccccgt
    39241 tatcgccagt ccagcccatc attaccgtct gatgctcgcc atctttcgca cgcttgaggt
    39301 attcacccca ttcgtaggtg acgattttgg cctgcacgcc cactttcgcc cagtctgcct
    39361 gaatcatctc cgccatgcgg cgagcattcg ggttatacgg acgttgtacc ggcattgccc
    39421 acaggtcgat ggagaaacct ttttccagac ccgcttcttt cagcaaggct ttcgcttttt
    39481 caggatcgta agtgtagtcc tgaacgtcgt cgttatagcc ccacatggtt ggcgggatca
    39541 ggtttttcgc tgatacgccc gcgccctgat aaaccgcttt gatgatagcg tctttgttca
    39601 ccgcgtaggt cagagcctgg cgaactttca cgtcatccag tggttttttc tgcacgttat
    39661 acgagagata accgacgttc agccccggca tttccatcag gttgatggat ttatcctgct
    39721 tcatgcgggc gatatctgcc gggttcgggt acggcatcac ctggcattcg ttcttctgca
    39781 atttcgcgta acgcacggaa gcgtcaggag taatagagaa gaccagcgta tcgatcttcg
    39841 gtttggtgcc ccagtagcca tcaaacgctt tatagcgaat acgggaatct ttctggtact
    39901 gctgtaactg gaacgggccg gtaccgattg ggttgaggtc cagtttttcc ggtgtaccgg
    39961 ctttcatcat cgcgtcagca tattcttttg acagaataga ggcgaagtcc attgccaggt
    40021 cagcgaggaa cggcgcttcc gggcgagtca gcacaaactg aacggtgtta tcgtccacct
    40081 ttttcacttc gctgatcagc tctggtaagc ccatgccttc gaagtattcg tagctgccgc
    40141 cagagacttt atggtacggg ttttgcgcgt ttttctgacg atcgaacgag aacactacgt
    40201 catcggcatt cagttcgcgc gtcggtttga attctttatt gtcgtgccac ttcacacctt
    40261 tacgcagatg gaaggtatag gttttaccgt cttcgctgac ttcccacttt tcagcgaggc
    40321 ccgggatcac ttcagtggtg ccgattttaa attcaaccag acggttataa agcggtacgg
    40381 aagaggcgtc ataggtggtg ccggaggtaa acagctgcgg gttaaaccct tccggagatc
    40441 cttctgagca ataaaccaga gttttagcct gaacacttgc tgcgacggtc atagccacca
    40501 ggctgagacc aagcttcagc atccctgact ttttcaagga aatacgcatt attctgctcc
    40561 aattgtgatg tttgttgttt taaccctttg cagtgggttg tcgctgcctg accttttttg
    40621 ttttttgccc ggtcgggtca acgttatgag gtggggatgc cgtactaatt aacatcagtg
    40681 taacagtgcg gatcctccat aaaatgcccc tcgtgaccta caatctgtca acagaatgtg
    40741 aaaacgtcaa tacagccaac cgggatttac accaacggtg agaatccaca aacaaagatt
    40801 aaaaaaactt caaacagcta tttgcagcag agaaatttgt gctacgccaa cacaaccaga
    40861 acaatcagct taatatttag caacatttgg tgattaagtt ttatgcattt aatgaaaaaa
    40921 atctgaggaa aaggtggata tctgagcgat taataccatg aacgctaaaa cgcacagcgg
    40981 aaaatgccag cgccagccat aagtaacgca aaaaaagatt taagcaaata taaaaaaaga
    41041 caatggtttc tgtcacaaat ccgttaatgg atagtgagat atggggcgca aatttggcag
    41101 ggaaatgtgt ggtgtgtaga tcggataagg cggtcgcgcc gaatccgaca atgggccggt
    41161 acatttgcct gatgcgacgc tggcgcgtct tatcaggcct acgtcgagtc gaacctcccc
    41221 gcccccggag gttctcttcc tcatgagtta cagatgcaaa aaagcctgct cgttgagcag
    41281 gcttttcgaa tttggtcggt gatagaggat tcgaacctcc gaccccttcg tcccgaacga
    41341 agtgcgctac caggctgcgc caatcaccga atgcggggcg catcttactg cgcagatacg
    41401 ccctcgtcaa tcccttaata gcaaaatgcc ttttgatcgg cgagaaagtc agcaggcctc
    41461 ttagttagcc gacgcctctt ttgcctgcgg gatgtgacac cagttgttgt tttcgttaat
    41521 tccaccatcc ggtgaagtat aaccaagaca acccatgatg gtgtcgtaca gctctacatg
    41581 acggcgtggc actttcatgt cggcttcttt tttcagctgc gcaaatgcct gcgcattgac
    41641 cggattttcc agatatttat ccgacatcca gaccatcatc ggcacacgga actgttccgg
    41701 cggtgccagt tcacgcggcg tgccgtgcag gtgctcgcgt tcattaattg actcaccgtg
    41761 gtcagctgca tagaacacaa tcgctttctt atcgcgaacc tgatcaatca cgctggaaat
    41821 aaagtgatcc acataggtca ccgagttgtc gtatgagttg atcatctgcg ctttggtaca
    41881 gccgctatca acaccaatac attccggctt ccactgcgca aagctgcgcg ggtaacgctg
    41941 ggtgtagtta aagtgtgagc ctttggtatg cagaatgatc agatgcttac catccgggtt
    42001 gcgccccagc gattgctgca tttcgtctac cagcaacata tcatctaccg gcttgccacg
    42061 gttacgtggc tccgcaccaa tctgctcacg ataagcaatg ttgtccgcca tcgtattgct
    42121 gtagaaccac atttcgcttt gcatagcgta gagatcagaa ctgaatccta actgcttcaa
    42181 taccgcgaaa atgttctgtt cttttaatgt gcgctgcgga ttatcttctg ctccaccctg
    42241 acgtacaaac atgcaacgca gtgagagttt ggttgcagta tcacaggagt aaccacggaa
    42301 cgccgccaga tttttctcct gagccagctt cggtgtggta ttgcgttcat agccgaaaat
    42361 gcccatatga tcccagcgcg tggtttcacc aataatgaaa acgacatatg tatcatcaac
    42421 gttttgtgga gcctggtacg taaacttctt cgcaggatta aggagagaat tgttatcgga
    42481 agattcatcg acccgcgccc aggcataaag acctaatgcc gaaagccagt ttgatggcag
    42541 ataagagttc gcgacgacgc cgccataact cggcaaatca acacctgtcg ccctctccac
    42601 tttcttctgc tggatatcca gcaaacgaat cggtgcccaa accataatac ctgccagtac
    42661 gacgaccgcc aggctgcgaa tacgttgccc tggggttcgc agttgtcgga gcaaggtgta
    42721 acgacagcgg ttattccaga taaggataag aggcaacgcg cttacggcga ttaaccagag
    42781 aataaagttc agaccaacaa cttcttttga caggtcgata tcggtggtca tgacggatgc
    42841 gatgatgcca taaccaatga ctacattaag gaaggtcatg taatagctgg cacctgcgga
    42901 aaagagcacc accagcgatg ccagaatacg ccagctgcgg cggccaaaca gcgaaagaag
    42961 acgcagtaaa aagaaggtca ccagtacggt ggcggccagt tcaacaacag cagaaatacc
    43021 tttccagacg gtaaaatcgt gcgcatagct gccgaagcgg cggtaaaaaa ccgcgccatt
    43081 cataaaaagg ccaatataga ttgcaagcaa gaagctcagc ttctgctgtg taatcgattt
    43141 gatgtatctc atgcaaacaa accctggaca aacaggtgat aacaccctgc ttagtggctt
    43201 ttcaggccag gcaggggaaa agtgcggagg tagtcagata agtaaaatct acacgcgcga
    43261 ataagtagac cacagcaaag ggaaaaagtg tggggaaaga gtgtgcacga agcacaaatt
    43321 tacaaaaatg caagtttgaa aagagacttt agcgttacaa aaagcagtaa aaagggccag
    43381 tgagctagcc ccggtgaagc gaaattattc gtactgaacc gtaaaatcga ccgtcgcatc
    43441 tgcgcggcca atggttgcat cagcacgcag cggcacgtag ctggcgatat agttcagttg
    43501 atgcgcgcca tcaccgttaa tcgcgaactc aatcggcgtc ggtgtcgtat ttttcgggaa
    43561 ctgttgtact cccgcagagg ttttaatttt caaccccacg cccgtagcac cacccgcatc
    43621 cagcgcaaaa agatttggat cgtccgcatt gggtgttcca tccatggtga cgtaagccct
    43681 gacaacttcg ctacagtttt tcagaccgat ggaaaaagta gtgggattaa gttctgttcc
    43741 gattcttact ggctttttga gaaccgctaa tcccatgtca acttcaacac tgtcttttac
    43801 cagttcacag gctccctggc tgattttaag agagaatttc aacgttcctt tcgcccctaa
    43861 gtctgtggtt gccgacagtg acgaaaagga aaaaggtagc aggcataatg caagtattgt
    43921 tcgtttcatc agtgctaatc caattcaaca tgcattgttg ccacaccgct ataatcaccg
    43981 gctgcgggta atttcccggt tgtactaacc ggataagcag taatggtggt tgttacttta
    44041 cgtttgacat attcgtacag tgagccggga ttcatttcta ataagctatt actatcattc
    44101 ggcttaagaa tattaccttt accatcctca attaccgcac caacatccgc gttgcccaaa
    44161 ctgattgcgc tggggtaagc cgcgtttggc gtaccttcaa gactcagata gatatggaca
    44221 ccatcagaaa tattagtaca gttaaacgtt aattcttttt gtattttctg gacattttgt
    44281 ggcgactgcc cggttcgacc tttgaaatca tgggcctgat actcaccaaa gtcgatatcc
    44341 aatgatgtcc ctgcggcaag ttcacagcct tgagttacgg tgacctttcc tgaaagaagc
    44401 acctgtgata ttggaactga actataaaca ttctcctttt ttgtaccaaa cacagaaact
    44461 ataatagttt gcggaatgtt ttgttcccca acaaaaggtt tagcgatata aagtgagagc
    44521 tgtcctttac tccctgtagt ccagccaaaa gttggttgat cgcactctct tcctggctgg
    44581 ctatttgatt tattgataaa tggaacctgt acattcccca cgtttgcaat atatacccgg
    44641 gttgaaacct caagattatt ggtaatttta aaataggttg agttatgccc cgccgccaat
    44701 gttgactcag ttttatatag cgttggtcgt gcttctgtcg ggttcggtga tggacattca
    44761 caaacaccag aatatttatc acccagtgcc cattggtaaa aatcagggta ggtctgcccg
    44821 gtaacattat ccgtcggatt ttgaatattt ttggtgaatg atgtaacaaa aataaagggg
    44881 tcaccacttg ccgtcctgca cggcccccaa tccgcagccc atatattccc ggtaaaggtg
    44941 ctcgtcccta aaagcaccat acctgccaac aagtttttaa ttttcatctg catatcctta
    45001 tttatcgcat accgcattaa ccatcacgat acccgaatta tcctttttct ctggcaagcg
    45061 gaatggcacg cggcaacgtt gcgcctgctc gtgcccccag ctcacctgca attcgccctc
    45121 ttctggcatc ccgctgatat aaagctgtcc ttcttcaccc acaatgctgc tggcaggttt
    45181 gctctcatca cttaacgctg cggtagcacc aaacggtact gcgttgccat tcgcccgttt
    45241 caaactcatc agcacacgat aaccgacacg cgtatcaaag cgggaacgga ctatcgcccc
    45301 acgcgttgga acgactttct gaaacgcttc ctgtaattca acgttttgcc ctaacgtgtc
    45361 gctgcgcaat tccacaatgg tttcacgata tggcgtgaga tacggcacca ctgcgttgcc
    45421 catccagtca acttcaacgc cgctgctatt tactacatgt ccaccacgcg cacccggcgc
    45481 agaaacaatt gccagacttt cacccaaggg ttgcgagaga gtgatacctt cactgtgaac
    45541 aatgactcca ccacgcacac catagttaac ttgttggtta tctttggaat agttataacc
    45601 cacgtcaatg ttgccagaac ctcccatata acccatgttg acactaccac tgttgccaac
    45661 cccattactg gtataaccct gctgaacgtt atagttcaag ttacgatctt ccagtaatgt
    45721 gccgctgaca gaggcctgtt gtgacgtctt gccgttctgg tcggtggtca tacgataact
    45781 gccccatgcg ccacccagcg gtattgagag gctaaatgac cacagtcgat cgtcttcatc
    45841 ccattcaggg cttctcgtcc aggtataggc gatactgtaa ttcacacgac caatatgacc
    45901 gtgataacca gcgcttaagg agttctcctt accatcaacg ttccagtaat cctgctgcgt
    45961 catattgaag taaacagaac cgtagtctcc cagttgctga gtgatgttac cttgtatctg
    46021 gctgcgctta tgataacgcc gatagtcact gtcggcgtca ctacggacat cggtcgcttc
    46081 ctggaaggtg taataaccgc tggttgagta cttgtagccc aacagacgca ggtcggtacc
    46141 gccctcaaaa cttttcgaat agaggaaacg ataagactgc ccttcatctt tttcattgtt
    46201 gcgtaactga cttttcgcct gggtgacatc caccgatacc gcaccaatgg tgccgaagtt
    46261 tttccccagc cccagagcta aagcgttgta gtctgccgat agcaacgcac caccataggc
    46321 ggtcattccc cacggcaagc cgtacatggc agtaaattgc ccgaatcgcg gtttaccgct
    46381 gtcgtaatta ccggcacgat actcaccggc agcaaggctg tatttcagat acccttcacg
    46441 ctggaaaatc gccactgccg aaaatggttg aataaaacgt tgttcacttc cgtcagattc
    46501 tttaatgatc accgtcaagt caccgctgcc ggaggttgcg tagagatcgt taatctcaaa
    46561 tgcgcccggc tgaacaaagg tacgataaat aacatatccg ttctgttcga tactcacttc
    46621 agcgttactt ttggcgatgc cacgcaccac cggagcaaag ccacgctggc tatccggcaa
    46681 catttcatcg tcggatgcca ataatgtccc gcgcatttgt acgctatcaa aaatctcgcc
    46741 aggcgtggcg gtatcaccca atgttatctg tgacttgagt ggaataatcg cccggtttaa
    46801 cgatgtacca acgttatccc attgattttt gccatcggta cggttccaga tactgttatt
    46861 acgtaaacgc cacggcccca cgttcaaacc gctgcgcaag ttcaggtaat aactgtcact
    46921 ggtcgaatct ggagatgaac cggaaccttc gttgcgacca ttgctgccac taaagctgta
    46981 gtccagtaac agcgcaggta ttccttcgtc ccagcgagaa ggatcaaccg tgccacgagc
    47041 ggtcatcatc attgctgcct gtggaaaggt aagatgcaac gtctgagtat taaaatcaaa
    47101 acgactggtg gcctggggaa tgatttcatc aaacgctacg caggcttccg gtggcaatat
    47161 cttcagtgcc ggaaagctgt cgatgcgcac gcccatttct tccagttgaa cacggctaag
    47221 gcaaggcata agcgactcgc cgctggcttt gcgttgtgct tcgctggcag tactaaaatc
    47281 cagcgtccgg gaagccattt tttcttcgtt tacaaacaca aatacccgat atttaccggg
    47341 tatttgctgg ccgacaggag agtcgtaaac tgacaaatcg acgtgttgat caataccctg
    47401 aacattttca agaaatttag ggttgaattt tccgccgtac gcaggtaacg cgctggcaat
    47461 agttaatgcc aacagtgaga aagaaaaatg cgctctgtag tgatcaagag gtaaatgcat
    47521 ccaatgcata gtaaagcgcc ttgtaaaaag atcagttaat cgacttcgtc agcatttcac
    47581 cggcgacacc gaaatcatta ataatgctgt atttcacttc gttcgcattg cctgtaccgg
    47641 ctggaaggtt aatgtttaac gttcctttag cgaggaccat tcccgcccgt tcaatctttt
    47701 gtccattaat ggacatctga ttaaaggtca ggttataagc tgagtcgtta ttaaccacaa
    47761 tttggttatt ttgacgctga aatcgtaact gtttcattgc ttcaaggttg cttcccggca
    47821 agcctttcgg acggtagaac aacttcaaac gggtgcgaac ggctatctgt aagacgtttt
    47881 tattttcatt ttcagcactt ttcgcaggaa tcgcttttac attaatccag taaacggatt
    47941 cacgatcttt gggtaaggta tcttcaatat taacaatacg taagacgtta tttttatcag
    48001 gatcaagttt gaataatggc ggtgtaataa taaacggcgt ttttttattt ccattcacat
    48061 catcaatcca tgactggatc aaaaactctt catttttatt atggttttca acggtcagcg
    48121 acgcttcttt cttgccacca tcataaataa cgcgcgtcgt gcttagggct acgccgccag
    48181 caaaacactg ttgtgcggtc attaaaagcg ccagaacggc aaggctaatt cgtttcatga
    48241 ttcggtccct gagtcattgg tgtgaataat ggcaacagat aatctgttgc cattgagaga
    48301 taatcgttcc gtctggatcg attagttgta agtcaggttg aagtcgactt cggcattacc
    48361 gtaaccgatg gttactgcat ttttggtagc gacatagtta gcggtaaaat acagaacagt
    48421 ctggccttct ttcaggacgg tagcagattt accggtattc agatcaacca gcgtattggt
    48481 attatcataa ataccaatac ccacgccagt tgcggcacct ggttcagtgc ttacagaaat
    48541 cagtttgctg ttgtctgcgt cgccaacacc gttaaagctg atctccacat ttttgaaagt
    48601 ggagatatcg cagttttcca gtttaatttt gaacggagtt gctgcagatt tatcaccagt
    48661 agtggtaaac acagacttgt tcacctggcc cagaacaact tcttgttttt gagtgtctgg
    48721 agacacaaca caagcggcgt caacaatctc tccggtgaat ttaacagaac cgtcgcctgc
    48781 atcagctgcg aatacggaag tagaggtcag cgcaagcgca gataaggcaa acagaacctt
    48841 tttcatttat aactccattt atataga
//
LOCUS       DADTFG010000014        45345 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.014, whole genome shotgun sequence.
ACCESSION   DADTFG010000014 DADTFG010000000
VERSION     DADTFG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.014"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            248..1195
                     /gene="cyoA"
                     /locus_tag="J1138_000828"
     CDS             248..1195
                     /gene="cyoA"
                     /locus_tag="J1138_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBA8452666.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMATFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1217..3208
                     /gene="cyoB"
                     /locus_tag="J1138_000829"
     CDS             1217..3208
                     /gene="cyoB"
                     /locus_tag="J1138_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBA8452667.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3198..3812
                     /locus_tag="J1138_000830"
     CDS             3198..3812
                     /locus_tag="J1138_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBA8452668.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3812..4141
                     /locus_tag="J1138_000831"
     CDS             3812..4141
                     /locus_tag="J1138_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBA8452669.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4153..5043
                     /gene="cyoE"
                     /locus_tag="J1138_000832"
     CDS             4153..5043
                     /gene="cyoE"
                     /locus_tag="J1138_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBA8452670.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5192..6556
                     /locus_tag="J1138_000833"
     CDS             5192..6556
                     /locus_tag="J1138_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452671.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGLPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6684..7175)
                     /gene="yajQ"
                     /locus_tag="J1138_000834"
     CDS             complement(6684..7175)
                     /gene="yajQ"
                     /locus_tag="J1138_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBA8452672.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7343..8254
                     /gene="panE"
                     /locus_tag="J1138_000835"
     CDS             7343..8254
                     /gene="panE"
                     /locus_tag="J1138_000835"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBA8452673.1"
                     /translation="MKITVLGCGALGQLWLTTLCKQDHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8217..8807
                     /gene="yajL"
                     /locus_tag="J1138_000836"
     CDS             8217..8807
                     /gene="yajL"
                     /locus_tag="J1138_000836"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBA8452674.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8861..10309)
                     /gene="thiI"
                     /locus_tag="J1138_000837"
     CDS             complement(8861..10309)
                     /gene="thiI"
                     /locus_tag="J1138_000837"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBA8452675.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGNSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10515..10757
                     /gene="xseB"
                     /locus_tag="J1138_000838"
     CDS             10515..10757
                     /gene="xseB"
                     /locus_tag="J1138_000838"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBA8452676.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10757..11656
                     /gene="ispA"
                     /locus_tag="J1138_000839"
     CDS             10757..11656
                     /gene="ispA"
                     /locus_tag="J1138_000839"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBA8452677.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            11681..13543
                     /gene="dxs"
                     /locus_tag="J1138_000840"
     CDS             11681..13543
                     /gene="dxs"
                     /locus_tag="J1138_000840"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBA8452678.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13598..14572
                     /gene="yajO"
                     /locus_tag="J1138_000841"
     CDS             13598..14572
                     /gene="yajO"
                     /locus_tag="J1138_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBA8452679.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSWEEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14626..15144)
                     /gene="pgpA"
                     /locus_tag="J1138_000842"
     CDS             complement(14626..15144)
                     /gene="pgpA"
                     /locus_tag="J1138_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBA8452680.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15122..16099)
                     /gene="thiL"
                     /locus_tag="J1138_000843"
     CDS             complement(15122..16099)
                     /gene="thiL"
                     /locus_tag="J1138_000843"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBA8452681.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16177..16596)
                     /gene="nusB"
                     /locus_tag="J1138_000844"
     CDS             complement(16177..16596)
                     /gene="nusB"
                     /locus_tag="J1138_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBA8452682.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16616..17086)
                     /gene="ribE"
                     /locus_tag="J1138_000845"
     CDS             complement(16616..17086)
                     /gene="ribE"
                     /locus_tag="J1138_000845"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBA8452683.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17175..18278)
                     /gene="ribD"
                     /locus_tag="J1138_000846"
     CDS             complement(17175..18278)
                     /gene="ribD"
                     /locus_tag="J1138_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBA8452684.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSVTVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18282..18731)
                     /gene="nrdR"
                     /locus_tag="J1138_000847"
     CDS             complement(18282..18731)
                     /gene="nrdR"
                     /locus_tag="J1138_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBA8452685.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18882..19421
                     /locus_tag="J1138_000848"
     CDS             18882..19421
                     /locus_tag="J1138_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBA8452686.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            19720..20604
                     /locus_tag="J1138_000849"
     CDS             19720..20604
                     /locus_tag="J1138_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBA8452687.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20642..20989)
                     /locus_tag="J1138_000850"
     CDS             complement(20642..20989)
                     /locus_tag="J1138_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBA8452688.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21119..22090)
                     /gene="secF"
                     /locus_tag="J1138_000851"
     CDS             complement(21119..22090)
                     /gene="secF"
                     /locus_tag="J1138_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBA8452689.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22101..23948)
                     /gene="secD"
                     /locus_tag="J1138_000852"
     CDS             complement(22101..23948)
                     /gene="secD"
                     /locus_tag="J1138_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBA8452690.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(23976..24308)
                     /gene="yajC"
                     /locus_tag="J1138_000853"
     CDS             complement(23976..24308)
                     /gene="yajC"
                     /locus_tag="J1138_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBA8452691.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24331..25458)
                     /gene="tgt"
                     /locus_tag="J1138_000854"
     CDS             complement(24331..25458)
                     /gene="tgt"
                     /locus_tag="J1138_000854"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBA8452692.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKLSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25513..26583)
                     /gene="queA"
                     /locus_tag="J1138_000855"
     CDS             complement(25513..26583)
                     /gene="queA"
                     /locus_tag="J1138_000855"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBA8452693.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26676..27257
                     /gene="acpH"
                     /locus_tag="J1138_000856"
     CDS             26676..27257
                     /gene="acpH"
                     /locus_tag="J1138_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBA8452694.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSERWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(27262..29079)
                     /gene="malZ"
                     /locus_tag="J1138_000857"
     CDS             complement(27262..29079)
                     /gene="malZ"
                     /locus_tag="J1138_000857"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBA8452695.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNAPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29235..30608)
                     /gene="proY"
                     /locus_tag="J1138_000858"
     CDS             complement(29235..30608)
                     /gene="proY"
                     /locus_tag="J1138_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBA8452696.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(30684..32003)
                     /gene="brnQ"
                     /locus_tag="J1138_000859"
     CDS             complement(30684..32003)
                     /gene="brnQ"
                     /locus_tag="J1138_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBA8452697.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32410..33705)
                     /gene="phoR"
                     /locus_tag="J1138_000860"
     CDS             complement(32410..33705)
                     /gene="phoR"
                     /locus_tag="J1138_000860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBA8452698.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTSD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVHNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(33763..34452)
                     /gene="phoB"
                     /locus_tag="J1138_000861"
     CDS             complement(33763..34452)
                     /gene="phoB"
                     /locus_tag="J1138_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBA8452699.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34642..35844
                     /gene="sbcD"
                     /locus_tag="J1138_000862"
     CDS             34642..35844
                     /gene="sbcD"
                     /locus_tag="J1138_000862"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBA8452700.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAYQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            35841..38984
                     /gene="sbcC"
                     /locus_tag="J1138_000863"
     CDS             35841..38984
                     /gene="sbcC"
                     /locus_tag="J1138_000863"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBA8452701.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWGQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSSSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKALAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAMK"
     gene            39110..40294
                     /gene="araJ"
                     /locus_tag="J1138_000864"
     CDS             39110..40294
                     /gene="araJ"
                     /locus_tag="J1138_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBA8452702.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(40563..41471)
                     /gene="mak"
                     /locus_tag="J1138_000865"
     CDS             complement(40563..41471)
                     /gene="mak"
                     /locus_tag="J1138_000865"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBA8452703.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYQQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            41596..42507
                     /gene="rdgC"
                     /locus_tag="J1138_000866"
     CDS             41596..42507
                     /gene="rdgC"
                     /locus_tag="J1138_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBA8452704.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLAGEAQR"
     gene            complement(42588..42668)
                     /locus_tag="J1138_000867"
     CDS             complement(42588..42668)
                     /locus_tag="J1138_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452705.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(42665..43003)
                     /locus_tag="J1138_000868"
                     /pseudo
     CDS             complement(42665..43003)
                     /locus_tag="J1138_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43151..43435)
                     /gene="ppnP"
                     /locus_tag="J1138_000869"
     CDS             complement(43151..43435)
                     /gene="ppnP"
                     /locus_tag="J1138_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBA8452706.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43507..44184)
                     /locus_tag="J1138_000870"
     CDS             complement(43507..44184)
                     /locus_tag="J1138_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBA8452707.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPLRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(44442..44633)
                     /gene="yaiA"
                     /locus_tag="J1138_000871"
     CDS             complement(44442..44633)
                     /gene="yaiA"
                     /locus_tag="J1138_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBA8452708.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(44683..45207)
                     /gene="aroL"
                     /locus_tag="J1138_000872"
     CDS             complement(44683..45207)
                     /gene="aroL"
                     /locus_tag="J1138_000872"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBA8452709.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VADIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT        11067 a        12073 c        11497 g        10708 t
ORIGIN      
        1 aaataattgt tttattttat attgattata ccaattgccc gcccaaactc cttctgcact
       61 cccctttcgt tataacgccc ttttgcaaca gcttcttaaa atcaacttga tatgttttgc
      121 aacatatgtg acctgacagc caaatccaag taacaggaat ttaatcatgt ttacagtaat
      181 gtaaccttcc cgtaaaatgc ccacacactt taaacgccac cagatcccgt ggaattgagg
      241 tcgttaaatg agactcagga aatacaataa aagtttggga tggttgtcat tatttgcagg
      301 cactgtattg ctcagtggct gtaattctgc gctgttagat cccaaaggac agattggtct
      361 ggagcaacgt tcactgatac tgacggcatt tggcctgatg ttgattgtcg ttattcccgc
      421 aatcttgatg gctgttggtt tcgcctggaa gtaccgtgcg agcaataaag atgctaagta
      481 cagcccgaac tggtcacact ccaataaagt ggaagctgtg gtctggacgg tacctatctt
      541 aatcatcatc ttccttgcgg tactgacctg gaaaaccact cacgctcttg agcctagcaa
      601 gccgctggca cacgacgaga agcccattac catcgaagtg gtttccatgg actggaaatg
      661 gttcttcatc tacccggaac agggcattgc taccgtgaat gaaatcgctt tcccggcgaa
      721 cactccggtg tacttcaaag tgacctccaa ctccgtgatg aactccttct tcattccgcg
      781 tctgggtagc cagatttatg ccatggctgg tatgcagact cgcctgcatc tgatcgccaa
      841 cgaacccggt acttatgacg gtatctccgc cagctacagc ggcccgggct tctcaggcat
      901 gaagttcaaa gctattgcaa caccggatcg cgccgaattc gaccagtggg tcgcaaaagc
      961 gaaacagtcg ccgaacacca tgtctgacat ggctacgttc gaaaaactgg ccgcgcctag
     1021 cgaatacaac caggtggaat atttctccaa cgtgaaacca gacttgtttg ccgatgtgat
     1081 caacaagttt atggctcacg gtaagagcat ggacatgacc cagccagaag gtgagcacag
     1141 cgcacacgaa ggtatggaag gcatggacat gagccacgcg gaatccgccc attaaagggg
     1201 ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt tccatgaacc
     1261 tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg ttggcctgat
     1321 cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg ttgaccataa
     1381 acgtctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg gttttgctga
     1441 cgccattatg atgcgtagtc agcaggccct tgcctctgcg ggcgaagcgg gcttcctgcc
     1501 acctcaccac tacgatcaga tcttcaccgc gcatggcgtg attatgatct tcttcgtggc
     1561 gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg gcgcgcgtga
     1621 cgttgcgttc ccgttcctca acaacttaag cttctggttt accgttgttg gtgtgattct
     1681 ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg cctatccacc
     1741 gctatcggga atagagtaca gtccgggagt cggtgtcgat tactggatat ggagtctcca
     1801 gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca ttctgaagat
     1861 gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctgggcat cactgtgcgc
     1921 gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt tgttgaccct
     1981 ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca tgatgatgta
     2041 tatcaacctg atttgggcct ggggccaccc ggaagtttac atcttgatcc tgcctgtatt
     2101 cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg gttatacctc
     2161 gctggtatgg gcaaccgtct gtatcaccgt gctgtcgttc atcgtctggc tgcaccactt
     2221 ctttacgatg ggtgcgggcg cgaacgtaaa cgccttcttt ggtatcacca cgatgattat
     2281 cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc agggccgcat
     2341 cgtgttccat tctgcgatgc tgtggaccat cggttttatc gtcaccttct cggtgggcgg
     2401 gatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata acagcctgtt
     2461 cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct gcttcgcagg
     2521 gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct ggggtaaacg
     2581 cgcgttctgg ttctggatca tcggcttctt cgttgccttt atgccactgt atgcgctggg
     2641 cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc acaccatgct
     2701 gatgattgca gccagcggtg cggtactgat tgcgctgggt attctctgcc tcgttattca
     2761 gatgtacgtt tctattcgtg accgcgacca gaaccgtgac ctgactggcg acccgtgggg
     2821 tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact ttgccgttgt
     2881 gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa gagaaaggcg aagcgtacaa
     2941 aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgccg gtatcgtcat
     3001 tgcggctttc tccaccatct tcggtttcgc catgatctgg catatctggt ggctggcgat
     3061 tgttggcttc gcaggcatga tcatcacctg gatcgtgaaa agcttcgacg aggacgtgga
     3121 ttactacgtg ccggtggcag aaatcgaaaa actggaaaac cagcatttcg atgagattac
     3181 taaggcaggg ctgaaaaatg gcaactgata ctttgacgca cgcgactgcc cacgcgcacg
     3241 aacacgggca ccacgatgca ggcggaacca aaatcttcgg attttggatc tacctgatga
     3301 gcgactgcat tctgttctct atcttgtttg ctacctatgc cgttctggtg aacggcaccg
     3361 caggtgggcc gacaggtaaa gacattttcg aactgccgtt cgttctggtt gaaactttct
     3421 tgctgttgtt cagctccatc acctacggca tggcggctat cgccatgtac aaaaacaaca
     3481 aaagccaggt gatctcctgg ctggcgttga catggttgtt tggtgccgga tttatcggga
     3541 tggaaatcta tgaattccat cacctgattg ttaacggcat gggtccggat cgcagcggct
     3601 tcctgtcagc attctttgcg ctggtcggca cgcacggtct gcacgtcact tccggtctta
     3661 tctggatggc ggtgctgatg gtgcaaatcg cccgtcgcgg cctgaccagc actaaccgta
     3721 cccgcatcat gtgcctgagc ctgttctggc acttcctgga tgtggtttgg atctgtgtgt
     3781 tcactgttgt ttatctgatg ggggcgatgt aatgagtcat tctaacgcaa gcagcggcgc
     3841 atcccatggc agcgtaaaga cctacatgac aggctttatc ctgtcgataa tcctgacggt
     3901 aattccgttc tggatggtga tgacaggggc tgcctctccg gccgtaattc tgggaacaat
     3961 cctggcaatg gcagtggtac agattctggt gcatctggtg tgcttcctgc acatgaatac
     4021 caaatcagat gaaggctgga atatgacggc atttgtcttc accgtgctaa tcatcgccat
     4081 cctggttgtg ggctccattt ggattatgtg gaacctcaac tacaacatga tgatgcacta
     4141 agagcggcgg ttatgatatt taagcaatac ctgcaagtaa cgaaaccagg catcatcttt
     4201 ggcaacctga tctcggtgat tgggggattc ttgctggcct caaagggcag cattgattat
     4261 cccctgttta tctacacgct ggttggggtg tcactggttg tggcgtcggg ttgtgtgttt
     4321 aacaactaca tcgacaggga tatcgaccga aagatggaaa ggacgaagaa tcgggtgctg
     4381 gtgaaaggcc tgatctctcc tgctgtctcg ctggtgtacg ccaccttgct gggtattgct
     4441 ggctttatgc tgctgtggtt tggcgcgaat ccgctggcct gctggctggg ggtgatgggc
     4501 tttgtggttt atgtcggcgt ttatagcctg tacatgaaac gtcattctgt ctacggcaca
     4561 ttgattggtt cgctctccgg cgctgcgcct ccggtgatcg gttactgtgc ggtaaccggc
     4621 gagttcgata gcggcgcagc gatcctgctg gctatcttca gcctgtggca gatgcctcac
     4681 tcttatgcca tagccatttt ccgctttaag gattatcagg cggcaaacat tccggtactg
     4741 ccggtggtga aaggcatttc ggtggcgaag aatcacatca cgctgtatat catcgccttt
     4801 gccgttgcca cgctgatgct ctctcttggc ggctacgctg ggtataaata tctggtggtc
     4861 gccgctgcgg tgagcgtctg gtggttaggc atggctctgc gcggctataa agttgctgat
     4921 gacagaatct gggcgcgcaa gctgttcggt ttctctatca tcgccatcac tgccctctcg
     4981 gtgatgatgt ccgttgattt tatggtgccg gactcgcata cgctgctggc tgctgtgtgg
     5041 taacacaacc tctcagttaa aaaggtgcta cggcaccttt tttcttagca ttagaatcat
     5101 atccctctcg aaatatttac taaaaaatcc gcatgtttac cccattcgtt tgccgcttta
     5161 cactagtcgc gaatttaaaa cagaggtggt aatgaacgat tataaaatga cgccaggtga
     5221 gcggcgcgcg acctggggtt tagggaccgt attttcatta cgcatgctgg gcatgttcat
     5281 ggttctgccg gttctgacca cgtacggcat ggctctgcaa ggtgccagcg aagcattaat
     5341 cggtattgcc attggtattt atggtctgac tcaggccgtt tttcagattc cgtttggcct
     5401 gctttcagac cgcattggtc gcaaaccatt aattgtcggt gggctggcgg tgtttgccgc
     5461 cggtagcgtt atcgctgcgc tctctgactc catctgggga attattctgg gccgggcgct
     5521 acaaggctcc ggtgcgattg ccgctgccgt tatggcgctg ctttccgatc tcacgcgcga
     5581 acaaaaccgc accaaagcga tggcgtttat cggcgtgagc tttggcatta cctttgccat
     5641 tgcgatggtg cttggcccga tcatcactca caaacttggg ctgcacgcgc tgttctggat
     5701 gatcgctatt ctggcaacaa ccggcattgc gttgaccatt tgggttgtgc ccaacagtag
     5761 cactcacgta cttaatcgtg agtccggaat ggtgaaaggc agtttcagta aagtgctggc
     5821 ggaaccgcgg ctgctgaaac tcaactttgg cattatgtgt ctgcatattt tgctgatgtc
     5881 gacctttgtt gccctgcccg gacagatggc tgatgcgggg ttaccggcgg ctgaacactg
     5941 gaaggtctac ctggcaacga tgttaatcgc ctttggctcg gtcgtgcctt tcattatcta
     6001 cgctgaagtt aagcgcaaaa tgaagcaagt ctttgtcttc tgcgtcgggt tgatcgtggt
     6061 tgcggaaatt gtgttgtgga acgcacaaac gcagttctgg caactggtgg tcggcgtgca
     6121 gcttttcttt gtggcgttta atttgatgga agcccttctg ccttcactta tcagtaaaga
     6181 atcgccagca ggttacaaag gtacggcgat gggtgtttac tccaccagcc agtttcttgg
     6241 cgtggcgatt ggcggttcgc tgggcggctg gattgacggc atgtttgacg gtcagggcgt
     6301 atttctcgct ggcgcaatgc tggccgcagt gtggctggca gtcgccagta ccatgaaaga
     6361 accgccgtat gtcagcagct tgcgcattga aatcccggcg aacattgccg caaacgaggc
     6421 gttaaaagtg cgtttgctgg aaactgaagg catcaaagaa gtgttgattg cagaagaaga
     6481 acattcagct tatgtgaaaa tcgacagcaa agtgacgaat cgctttgagg ttgaacaggc
     6541 aattcgccag gcataaaagc gaggcgaaat tgcctgctgc gctacgctta tcaggcctac
     6601 taagttcctg agttacaaac ctttgtaggc cgaatcaggc gttcatgccg catctgacat
     6661 gaacaaagcg caggttgtcg cgattaatcg cggaagtttt tgaactggaa cggctgaccg
     6721 agatcgccac cacgtaccat cgccatgaca gcctgtaaat catcacgaga tttacccgtt
     6781 acgcggatct catcgccctg aatttgcgcc tgcactttca gtttgctgtc tttgatcatt
     6841 ttgacgattt tcttctgagt cgcgctctca atgccctgtt tcagtttcgc ttccacaaac
     6901 caggttttac cgctatgaac gatattttcc ggtacatcca gtgaactgcc ttcaatgccg
     6961 cgcttcagca acttggcacg cagaatatcc agcaactgat tgacctggaa gtcggactcg
     7021 ctcaacactt tgatggtttt gctggcgtca ttcagctcaa atgaggcttc aacgttacgg
     7081 aagtcaaaac gggactccac ttcgcggctc gcgttatcga ccgcgttacg tgcttcctga
     7141 agatcaactt cagagacaat atcgaaagat ggcatctttt cctctccctt catttttgat
     7201 gcgaagcata atacctgcaa agttaaataa ccaacagtcc tgcaccagga cgtatccctt
     7261 aattgtgcat agtcgcaaca gcatgcacaa ttaagggata gccttattga acacccggag
     7321 tggttgcggg tgaggaggaa caatgaaaat taccgtattg ggatgcggtg ccttagggca
     7381 attatggctt acaacacttt gtaaacagga tcacgacgtt cagggctggc tgcgcgtacc
     7441 gcaaccttat tgtagtgtga atctggttga gacagatggt tcgatattta acgaatcgct
     7501 gaccgccaac gatcccgatt ttctcgccac cagcgatctg ctcctggtga cgctgaaagc
     7561 atggcaggtt tccgatgccg tcaaaagcct cgcgtccaca ctgcctgtaa ccacgccaat
     7621 actgttaatt cacaacggca tgggcaccat cgaagagttg caaaacattc agcagccatt
     7681 actgatgggc accaccaccc atgccgcccg ccgcgacggc aatgtcatta ttcatgtggc
     7741 aaacggtatc acgcatattg gtccggcacg gcaacaggac ggcgattaca gttatctggc
     7801 ggatattttg caaaccgtat tgcctgacgt cgcgtggcat aacaatattc gcgctgagtt
     7861 gtggcgcaag ctggcagtca actgtgtgat taatccactg accgccatct ggaattgccc
     7921 gaatggtgaa ttacgtcatc atccgcaaga aattatgcag atatgcgaag aagtcgcggc
     7981 agtgatcgaa cgcgaagggc atcatacttc agcagaagat ttgcgtgatt acgtgatgca
     8041 ggtgattgat gcaacagcgg aaaatatctc gtcgatgttg caggatatcc gcgcgctgcg
     8101 ccacaccgaa atcgactata tcaatggttt tctcttacgc cgcgcccgcg cgcatgggat
     8161 tgccgtaccg gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga gtgaatatga
     8221 gcgcatcggc actggtttgc ctcgcccctg gtagtgaaga gactgaagcc gtcaccacta
     8281 tcgacctctt ggttcgtggc ggtatcaaag tcaccactgc cagcgtcgcc agcgatggca
     8341 acctggcaat tacctgctcg cgcggcgtga agctgctggc ggatgcgccg ctggtcgaag
     8401 tggctgacgg tgaatatgac gtgatcgtgc tgcctggtgg cattaaaggc gcagagtgtt
     8461 ttcgcgatag cactctgctg gttgaaaccg ttaaacagtt ccaccgttcc gggcgtatcg
     8521 tcgcggctat ttgcgccgcg ccagccaccg tgctggtccc gcacgatatc ttcccgattg
     8581 gcaatatgac cggcttcccg acgctgaaag acaaaatacc cgccgaacaa tggcaggaca
     8641 agcgcgtcgt ctgggatgca cgggtaaaat tgctgaccag ccaggggcca ggtacagcta
     8701 tcgactttgg tctgaaaatt atcgacctgc tggttgggcg cgagaaagcc catgaagtgg
     8761 catcacaact ggtgatggcg gcagggattt ataattatta cgagtagtgt cggatgcggt
     8821 aaacgtcgca tctgaccaga tgcgacgtta caaccacata ttacgggcga tacaccttca
     8881 cattgttaaa gccctgctcg cgcagataga gcgcctgcag gcggctcatc accccgcgct
     8941 cacaccacaa cagccaggtt ttgctctggt cgagatcgcc aaatttggtg ctcagtttat
     9001 agaacggcag agaaaccaca tcaatccctt cgactttcag tggcttatct tcctgttcat
     9061 cgatagaacg gatatcgagg atcacgtcgt tcgggccgaa gccattgacg gtttccactt
     9121 ccaccacttc ctgctcggtc tgctgggcga tttcgcggat atcaacgtta ttcgcttcct
     9181 ccaccacttt atcgagaatg ctgaagtcga acttctcttc ttccgcttca atcttcgatt
     9241 taaccgcttt caccgtcggg cttttggaaa tcacgccgca atattccggc atcgtgcggg
     9301 caaagtcttc ggtgccaatc tggcgagcca ggttgatgat gtgctctttg tcgtaagaga
     9361 tcagcggacg caggatcagc gtatcggaga cgttatcaat caggcgtagg ttggtcagcg
     9421 tctggctgga cacctggccg agcgcttcac cggtaaccag cgcctgtacg ccgtaacgtt
     9481 ctgccacttt ggacgcggca cgcaccatca tacgtttgag gataacgccc atctgaccgt
     9541 cgtcgatttt ctcgagaatt tccccgacga ccggttcgaa attaatagcg acaaaacgca
     9601 cgcggtggga gttgccaaag cggttccaca gataatgcgc cacctgacgt acgccaattt
     9661 catgcgccgc gccgccgagg ttaaagaagc agtaatgcac gcggcagccg cgacgcatca
     9721 acatataact ggaaacaccg gagtcgaaac caccggaaat gagcgacaac acatcttcct
     9781 gggtgccgat cgggaaaccg ccaatacctt cgtagcggcc tttaatcagc agcagacgat
     9841 cgtcttccac ttccagatgg acagtcactt ccggattagt cagcttcacg cgcgcggatt
     9901 caatatgctg atttaaaccg ccgccgacgt aacgttccac atcaatcgag ctaaaatcat
     9961 gtttgccgcg gcgcttcacg cgtacgcaaa aggttttgcc ttccagctga tcgcgatatt
    10021 gaaccaacgc tttctcgaaa atatcgtgca tgtcggtaaa cggcacgtct tcgacttcga
    10081 gaatatggtg gatacccgga atacgggtca gggcgtcgcg aatagccaga cgctggtttt
    10141 catcttttgc gcgaacttcg atgttatccc agtggcggac gacagcgagc gtctcatcat
    10201 agtgctttaa aacgttacga atgttcccgg taaggatttt tataaagcgc aagcgcacag
    10261 attggctttt gatggtgatt tccgggaaca atttaatgat aaacttcatg gcggcaatgg
    10321 ttcgttggca agccttaagc gacttgtata gggaaaaata cagcagccca cacctgcggc
    10381 tgcatccagg cgcggaagta taccactaac atcgctttgc tgcgcacatc accttaccat
    10441 tgcgcgttat ttgctatttg ccctgagtcc gttaccatga cggggcgaaa aatattgaga
    10501 gtcagacatt cattatgccg aagaaaaatg aggctcccgc cagctttgaa aaggcgctga
    10561 gcgagctgga acagattgta acccgtctgg aaagtggcga cctgccgctg gaagaggcgc
    10621 tgaacgaatt cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa ttacaacaag
    10681 ccgaacagcg cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta acccctttta
    10741 caccggacaa tgagtaatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa
    10801 ccaggcgctg agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac
    10861 catgcagtat ggcgcattat taggtggtaa gcgcctgcga cctttcctgg tttatgccac
    10921 tggtcatatg ttcggcgtta gcacaaacac actggacgca cccgctgccg ccgtagagtg
    10981 tatccacgct tactcattaa ttcatgatga tttaccggcg atggatgatg acgatctgcg
    11041 tcgcggtttg ccgacctgcc atgtgaagtt tggcgaagca aacgcgattc tcgctggtga
    11101 cgctttacaa acgctggcgt tctcgattct aagcgatgcc gatatgccgg aagtgtcaga
    11161 tcgcgacaga atttcgatga tttctgagct ggcgagcgcc agcggtattg ccggaatgtg
    11221 cggtggtcag gcattagatt tagacgctga aggcaaacac gtacctctgg acgcgcttga
    11281 gcgtattcat cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt
    11341 aagcgccgga gataaaggac gtcgtgctct gccagtactc gacaagtacg cagagagcat
    11401 cggccttgcc ttccaggttc aggatgacat cctggatgtg gtgggagata ctgcaacgtt
    11461 gggaaaacgc cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg
    11521 tcttgagcaa gcccggaaga aagcccggga tctgatcgac gatgcccgtc agtcgctgaa
    11581 acaactgact gaacagtcac tcgatacctc ggcactggaa gcgctagcgg actacatcat
    11641 ccagcgtaat aaataaacaa taagtattaa taggcccctg atgagttttg atattgccaa
    11701 atacccgacc ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag
    11761 tttaccgaaa ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag
    11821 cgggcacttc gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta
    11881 caataccccg tttgaccagt taatctggga tgtggggcat caggcttatc cgcataaaat
    11941 tttgaccggg cgccgcgaca aaatcggcac catccgtcag aaaggtggcc tgcacccgtt
    12001 cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc gggcattcat caacctccat
    12061 cagtgccgga attgggatcg cagttgctgc cgagaaagaa ggcaaaaatc gccgcaccgt
    12121 ctgtgtcatt ggcgatggcg cgattactgc tggcatggcg tttgaagcga tgaatcacgc
    12181 gggcgatatc cgtcctgata tgctggtggt tctcaacgac aatgaaatgt cgatttccga
    12241 aaatgttggc gcgctcaaca accatctggc gcagctgctt tccggtaagc tttactcttc
    12301 actgcgcgaa ggcggaaaaa aagttttctc tggcgtgcca cccattaaag agctgctcaa
    12361 acgtaccgaa gaacatatta aaggcatggt agtgcctggc acgttgtttg aagagctggg
    12421 ctttaactac atcggcccgg tggacggtca cgatgtgctg gggcttatca ccacgcttaa
    12481 gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg
    12541 ttatgaaccg gcagaaaaag acccaatcac cttccacgcc gtgcctaaat ttgatccctc
    12601 cagcggttgt ctgccgaaaa gtagcggcgg tttaccaagc tattcaaaaa tctttggcga
    12661 ctggttgtgc gaaaccgcag cgaaagataa caagctgatg gcgattactc cggcgatgcg
    12721 tgaaggttcc ggcatggtcg agttttcacg taaattcccg gatcgctact tcgacgtggc
    12781 aattgccgag caacacgcag tgacctttgc tgcgggtctg gcgattgggg gctacaaacc
    12841 cattgttgcg atctactcca ccttcctgca acgcgcctat gatcaggtgc tgcatgacgt
    12901 ggcgattcaa aagcttccgg tcctgttcgc catcgaccgt gcgggcattg ttggtgctga
    12961 cggtcaaacc caccagggcg cttttgatct ctcttacctg cgctgcatac cggaaatggt
    13021 cattatgacc ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta
    13081 taacgatggc ccgtccgcgg tgcgctaccc gcgcggcaac gcagttggcg tggaactgac
    13141 gccactggaa aaactgccaa ttggcaaagg cattgtgaag cgtcgtggtg agaaactggc
    13201 gatccttaac tttggtacgc tgatgccaga agcggcgaaa gtcgctgaat cgctgaacgc
    13261 tacgctggtc gatatgcgtt tcgtgaaacc gcttgatgaa gcgttaattc tggaaatggc
    13321 cgccagccat gaagcgctgg ttaccgtaga agaaaacgcc attatgggtg gtgcaggcag
    13381 cggcgtgaac gaagtgctga tggcccatcg taaaccagta cccgtgctga acattggtct
    13441 gccggacttc tttattccac aaggaactca ggaagaaatg cgcgccgaac tcggcctcga
    13501 tgccaccggt atggaagcca aaatcaaagc ctggctggca taatccctac tctactcctg
    13561 ctatgcttaa gaaattattc atggcaggag tggcagcatg caatacaacc ccttaggaaa
    13621 aaccgacctt cgtgtttccc gactttgcct cggctgtatg acctttggcg agccagatcg
    13681 cggtaatcac gcatggacac tgccggaaga gagcagccgt cccatcatta aacgtgcgct
    13741 ggaaggcggc ataaatttct ttgacaccgc caatagctat tccgatggca gcagcgaaga
    13801 gatcgtcggt cgcgcactgc gggatttcgc ccgtcgtgaa gacgtggtcg ttgcgaccaa
    13861 agtgttccat cgcgttggtg atttaccgga aggattatcc cgtgcacaaa ttttgcgctc
    13921 tatcgacgac agcctgctcc gtctcggcat ggattatgtc gatatcctgc aaattcatcg
    13981 ctgggattac aacacgccga tcgaagagac gctggaagcc ctgaacgacg tggtaaaagc
    14041 cgggaaagcg cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg ctcaggcact
    14101 ggaactacaa aaacagcacg gctgggcgca gtttgtcagt atgcaggatc actacaatct
    14161 gatttatcgt gaagaagagc gcgagatgct gccgctgtgt tatcaggaag gcgtggcggt
    14221 gattccatgg agcccgctgg cgcggggccg tctgacgcgt ccttggggag aaaccaccgc
    14281 acggctggtg tcggatgaag tagggaaaaa tctctaccaa gaaagcgatg aaaatgacgc
    14341 acaaattgca gaacgcttaa cgggcgtcag tgaagaactt ggtgcaacgc gagcacaagt
    14401 tgcgctggcc tggttgttga gtaaaccggg cattgccgca ccgattatcg gaacttcttg
    14461 ggaggaacag cttgatgaac tgctaaacgc ggtggatatc accttgaagc cggaacagat
    14521 cgccgagctg gaaacgccgt ataaaccgca tccggtcgta ggatttaaat aggtatacag
    14581 gcctgatgag acgtgacaag cgtcacatca ggcatcggtg cacaactacg acagaatacc
    14641 cagcggccag tgatgtccaa taaagtacag gatgcctgcg gaaatcaccc cggcgacaat
    14701 atcgtcaatc atgatcccca tgccgccatg tacattgcga tcaaaccagc ggatcggcca
    14761 cggcttccac atatccagaa tacggaaaat cacaaatccg gcggtaaccc actgccagtc
    14821 attggtcggc agcgccatga gcgtaatcca cataccaata aattcgtccc agacaatact
    14881 gccgtgatcg tgcacgccca tgtctttcgc cgtttgatga caaagataga cgccgataca
    14941 gatccccagc attaccacca gcgaatagag ctgccagggc aaaaaagtca tcagatacca
    15001 gaagggaatc gccgccagcg accccatcgt gccaggaacg atcgggctta atccacttcc
    15061 gaatccgaca gcaagcagat gccacggatt actcattttc aggcgacttt tcgcgacatc
    15121 tttatggcgt ggcaaaatgg tcatatcctt tccagtctaa cgtgacaggt tcgccgtcac
    15181 gaataaaaca aagcccttcg atatcggcgg tcatttgccc gatacaggta aacggtacgc
    15241 ccaggtgtcc gagagccaca tccagcgcgc cacggttcag ttccggcacc gtgaaacaca
    15301 actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc ctgttccggt tcaacatggc
    15361 gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg cagtcgctgg
    15421 ctttcacgat atgcccgaga tcggaaatca gaccgtcaga gagatcgata gctgaatttg
    15481 ccagatcgcg cagtgcctgc ccctgtaaaa tacgcggcga cggacggaga tgacgtttga
    15541 tcaagtagtc cgcatcttta gcatcggcaa cctgcaaacg gttttgcaaa atcgccagcc
    15601 cggcagcgct atcgcccggt gtaccggtca catagatcca gtcacccggt ttcgccccag
    15661 agcgcgttaa ggctcgtccc atcggaacaa agccgtggat acccaacgtc attgataatg
    15721 gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta ataattgaga agatcaaaca
    15781 agctgtcgct gaaggactca agccacgctt cgtctacgtc cggtaaggtt aatgccagcg
    15841 tcagccaggc cggatcggcc cccatcgctg ccagatcgct taggttcacc gccagtgctt
    15901 tataagccag atcagcagga tcgatatcag ggaggaagtg gttgcccgcc accagcgtat
    15961 cagtgctgat cgccagggtc tgtttctcag ggatattgag aagagcgcaa tcgtcgccga
    16021 tgcccagttc gacatcaaga cgagaacttc ttacacggtc aaaataacgg gcaatcaggg
    16081 agaactcgcc acatgccata cgttatgcct cagcaggtaa aaaagaaaag gccggcgatc
    16141 atggaacggt cttccgtgaa tctaccggcc tggatattac tttttgttag ggcgaatcac
    16201 aggtgctgct ttatcgagta cgccgttgac gaacttatgg ctgtcttctg cgccgaacga
    16261 tttcgccagt tcgatcgctt cgttaatggc cactttgtat ggcacatcgc tacgtttaga
    16321 cagctcgtac agcgcaatgc gcagtactgc tttttctacc tggcccagtt cttccagcag
    16381 acgggacagg tacggcttca tcagtccgtc gaggtatgcg gtgttagtcg ccaccccggc
    16441 cagcagctca cggaagtaca ggacgtcaac gtcttttaca tcctgttcag ccaggaactg
    16501 gtattcaaca tcagcgatgt cgttctggga caactgccag gagtagagcg cctggacggc
    16561 acactcacga gcgcggcgac gagcagcagg tttcacggat ttccccttac taatttcagg
    16621 ccttgatggc tttcaataca ttaatcattt caagcgcggt cagtgcagct tctgcacctt
    16681 tgttgccagc tttggtgcca gcacgttcga tcgcttgttc aatgctttca gtggtcagaa
    16741 caccaaaagc aaccggaatt tcgctgtcct gggcaacatg cgccaggccg ttgcttgcac
    16801 caccagcgac atattcaaag tgggcagtgc caccacgaat aaccgtaccc agcgcaatca
    16861 ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc cagcggcagc tcataggcac
    16921 ctggcaccca aacaacggta atgttttcat cttttacctg accgatacgt ttcagtgcgt
    16981 caattgcacc ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg atggtgatgg
    17041 cgacgcgagc gtccggggta gcaacgttag cttcaataat gttcattttc ttccttcggg
    17101 ttcgagtatg gccccgcagg ggggcggatt ttagcataat atttcgtgcg ctgcttccct
    17161 ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa catccgggcc tacatgacgt
    17221 atctctttga atttaaattg gggagcgtcg gctaatttct caagccctgg cagcgtgcat
    17281 aatccacggg cgtcgttgcc taatagttta ggtgcgatat agacaatcag ctcatcgact
    17341 aaacccgcct gtagcaatgc gccagcgagc gttggccccg cttccaccca gatgctgtta
    17401 atctgctgtt tacccagttg catcattagt acaaccagat ccaggtgacc tttatgctct
    17461 ggaatcagca aggtacgcac cgtttccggc cactcacgag aatcttcctg cgtacgcgcg
    17521 aaccaagttt cgccgggctg ctgcacaatg cgatgttccg gcgtcacgcg attttggcta
    17581 tcaatcacaa tacgtaccgg ctggcggaga ttttgttgcg ggtagagccc ctgagtttgt
    17641 tcatccagtt cagaccaacg cactgttaag gctggatcat ccgccagcac cgtgacgctg
    17701 ctggttaaaa tggcatgact ttgcgcgcgc agtcgttgta cgtcgcgcct cgcctgaggt
    17761 gaagtgatcc actggctttc gccactcgcc atcgccgtgc gaccatcaag cgatgcgcca
    17821 agttttaact gaatataagg aaagccggtg cgcatccgtt tgagaaagcc tttattcaat
    17881 tgctcagctt cactcatcat cagcccgtgg ctgacgtcaa tgccagcctg ttgcagacgg
    17941 taaagtccac gtccagcgac ctgcggattg gggtcttgca ttgcagcaac cacgcgcgcc
    18001 acgcccgcag caattaatgc atcacaacac ggtggcgtac gaccatgatg gctacagggt
    18061 tcgagtgtga catacgccgt tgcgcctttg gctttttcgc cggccatgcg caacgcgtgt
    18121 acctcggcat gcggctcacc cgctcgttga tggtaacctt caccgacaat ttcgccatct
    18181 ttgacaatga cgcacccgac attcgggttg ggatgcgtgg taaaacgtcc tcgttgcgcc
    18241 agctttagcg cccgcgccat gtaatactcg tcctgcacgg cttagtcctc caggcgcgcg
    18301 atctcttcgc caaattcttt gatatcttcg aaactgcggt agacagaggc aaaacggata
    18361 taggcgactt tatcgagctt tttcaattgc tccatcacca gattgccaat catcttgctc
    18421 ggcacttcgc gctcaccggt ggcgcgcagc tgcgatttta tatgattgat tgccatttcg
    18481 acgtcatcgg aactcaccgg acgtttttcc agcgcccgca gcattccgct acgtaatttc
    18541 tcttcattaa acggttcacg cacgtcgttg ctttttacaa cacgcggcat aaccagctcc
    18601 gccacttcaa aggtggtgaa acgttcatta cacaccagac actgccgacg gcggcgtacg
    18661 gatgaaccct cgcccacgag acgagagtca attaccttag tgtccacggc gaaacagaat
    18721 gggcaatgca tacggcgtcc tgaccaggtg gttaacagaa atctatttta acctgaactg
    18781 acgatacaac aaaggcgcaa cgcttttgag ataaaggatc gatgctttca ctgcttttac
    18841 cctctaccat taggctaatt gcgacgagaa agggacgacc tatgaatact aacgtttttc
    18901 gactgctcct gctgggaagc ctgttcagcc ttagcgcctg tgtgcagcaa agtgaagtgc
    18961 gacagatgaa acacagcgtc agcacgctga accaggagat gacgcagctc aataaagaaa
    19021 cggtcaaaat cactcagcaa aacaggctga atgcaaaatc cagcagtggg gtttaccttc
    19081 tgcctggagc aaaaacacca gcaagactgg aaagccagat cggtacttta cgtatgtcgc
    19141 tggtgaatat tacgcctgat acagatggca ccaccctgac gctacgtatt cagggcgaat
    19201 ccaatgaccc tttacccgca ttcagcggaa ccgttgaata tgggcaaatt cagggaacaa
    19261 tagacaactt tcaggaaatc aatgtgcaga atcaattgat taacgcacct gccagcgtcc
    19321 tcgcccccag cgatgttgat attccgttac agttaaaggg tatgtctgtg gatcagttag
    19381 gctttgtacg tatccacgac attcaacctg ttatgcagta aacgtattgc ggggcgatat
    19441 tgtgcgtccc gcaacatctt tctccgtcat tttgttactc cgcttacatc acctggattg
    19501 atagtaaaag tttgcaacaa gggtgaaagt cagtacaatc cccgcccgaa tgtgtgtaaa
    19561 cgtgaacgca atcgattacg taaatgatag aactgtgaaa cgaaacatat ttttgtgagc
    19621 aatgattttt ataataggct cctctgtata cgaaatattt agaaacgcaa tttgcgcctt
    19681 tttcactccc gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac attactggca
    19741 gccggtgcgg tactggcgct ctcttcgtct tttactgtca acgcagctga aaacgacaaa
    19801 ccgcagtatc tttccgactg gtggcaccag agcgttaacg ttgtcggaag ctatcacacc
    19861 cgtttcggac cgcagctccg caacgatacc taccttgagt acgaagcatt cgctaaaaaa
    19921 gactggttcg acttctatgg ttatgcggat gcgccggtat tcttcggcgg taactccgat
    19981 gcaaaaggta tctggaacca cggttctccg ctgtttatgg aaatcgaacc gcgtttctcc
    20041 atcgacaagc tgaccaatac tgaccttagc tttggtccgt tcaaagagtg gtacttcgcg
    20101 aacaactaca tttacgacat gggtcgtaat aaagatggcc gccagagcac ctggtacatg
    20161 ggtctgggta ccgacatcga tactggtctg ccgatgagcc tgtccatgaa cgtctatgcg
    20221 aaataccagt ggcagaacta cggcgcagcg aacgaaaacg agtgggacgg ttaccgtttc
    20281 aaagtgaagt actttgtgcc gattaccgat ctgtggggcg gccagctgag ctacatcggc
    20341 ttcaccaact tcgactgggg ttccgacctg ggcgatgaca gcggttatgc gaacaacggt
    20401 attaaaacac gtaccaataa ctctattgcg tccagccata ttctggcgct gaattacgat
    20461 cactggcact actctgtcgt tgcccgttac tggcataacg gtggtcagtg gaatgacgat
    20521 gcagaactga acttcggcaa cggcaacttc aacgttcgct ctaccggctg gggtggttat
    20581 ctggtagtag gttacaactt ctaattgtga gaatgccgga tttcatatcc ggcatttttt
    20641 atcacttctt cttgttcatc atcgctttca aatcagcgaa tgggttgtac ttcgcttcac
    20701 cgacatcttt ctgcgcatct tcccctgcga taacggtcgt accatactga tccgcttcgg
    20761 tgtatttcga atgctcatga tcgtggcaat agagacaaag taattcccag ttactgccat
    20821 cttccgggtt attagtatgg tcgtgatcaa tgtggtgaac ggtaagttca cgcaggttgg
    20881 aataaacaaa ctcgcgggaa cagcgaccgc agacccacgg atagattttt aatgcttttt
    20941 cgcgatagcc gctttctaac cgcgcatagt tttttgggat gatagccatg aaaaagttac
    21001 ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc gcaatcacct
    21061 tacatcaaaa aaaatcccga tcttctgacc gggattttca acatcagctg aaacttgatt
    21121 aaggcagaat tgacggctga tccgcccctt ctttttccac tttctgctgc aacatgtgtt
    21181 cgcgcttcat acccagtttc agagccagcg cagatgccac atagatggaa gatgcagtac
    21241 cgatggaaac accgataagc atggtcagcg agaagccttc cagtaccgga ccaccgaaga
    21301 ggtacagcat caggataacc atcaaggtag taccggatgt gatcaaggta cggtgcagcg
    21361 tctgggtcaa ggacacgtta aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt
    21421 tttcacgaat acggtccgat accacgatac tgtcgttaag cgagtaaccg ataaccgaca
    21481 tcaacgatgc cacaatggtc aggtcaatct cgatatggaa taacgacaaa atacccagcg
    21541 taatgataac gtcgtgcgcc agcgcaataa caacccctgc cgccagtcgc cactcaaagc
    21601 ggaaacctac gtacacgagg atagacagca gcgccgccat caacgccatc gcaccggttt
    21661 gcgcaaggtc tgcccccacg ctcggaccga cgaactcaat acgcttcact gccgcattct
    21721 gattggtgga ttcgttaatc accttcagaa cctggctgcc cagcacctga ccgccggttt
    21781 cgccttcagc aggcggcata cggaccatga tgtcgtggct gctaccaaag ttttgcagca
    21841 tcggctcttc aaaaccggct ttttgcaatg catcacgcat tacgtcaatt tcagccggtt
    21901 tttcgagcgt aatttcaata accgtaccac cggtaaaatc cagcccccag ttaaagccgc
    21961 gcacgcccat aataacgata gcggcgatta acagcagacc agagatgccg aaagcccagt
    22021 agtcccagcg cataaagtca tagactttac ggccgtggtt tagttgttca acagtatatt
    22081 cctgtgccac atcgcactcc tcagattgac agcttcttga cgcgcttgcc gccatatagc
    22141 aggtttacga tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc cacaccgata
    22201 ccggtagtaa tcgcgaaccc tttaattgcc ccggtaccca ctgcatacag gatgatgact
    22261 ttaatcagcg tggtgatgtt cgcatcgaag atggaactga atgcgccacg ataaccttca
    22321 tcaattgcct gttgaacagt acgtccgttg ctcaactctt ctttaatacg ttcgttgatc
    22381 agtacgttcg catcgaccgc taccgcaagg gttaagacga tacccgcgat acctggcata
    22441 ctcagcgttg cgcctggcaa cagcgacata ataccgacaa tcaagatcaa gttggcaatc
    22501 agagcactgg tcgcaatcag accaaacttc ttatagaaga tgatcatgaa cagaatagaa
    22561 accagcagac cggcaaggca agcctccagc ccctgctcaa tgttctgcat acccagggtt
    22621 gggccaatgg tacgttcttc aacaatctga atcggcgcga tcaacgcacc ggcacgcagc
    22681 agcagtgaaa gctgacgcgc ttcgcttggg ttattgatgc cagtaatacg gaagctgtta
    22741 cccagacgag actggatgtt ggcgatgtta atcacctctt cctgtttcac cagaaccgca
    22801 cgaccgtttg catctttctt accgctgtct ttgtactcca caaacagagt tgccatcggt
    22861 ttgccgatgt tgtccttggt gaagttagac atgatgttac cacccgcgct atcgagcgag
    22921 atgttaacct gcggctggtt gtattcgtcc tggctggaag tggagtcggt gatatggtca
    22981 ccggtcagaa ttacgcgttt gtacagcaca accggctgac cttcgcgggt ctgtttcact
    23041 tcagagtcgc ccggtacgcg accggatgcc gcagcggcct gatcaacgtt ggtgtttacc
    23101 agacggaatt ccagcgttgc cgtcgcaccc agaatctctt tcgcacgcgc agtgtcctga
    23161 atacctggca gttcaacaac gatacggtca gcaccctgac gctgaaccac cggctccgcc
    23221 acgccaagtt ggtttacacg gttacgcagg atattgatgt tctgctgcac cgcatattca
    23281 cgcgcttcac tcagacgagc atcgctcatt accgcacgca gctggttgct gccctggctg
    23341 ctaatcacca ggtccggatg gcgcttgctc agatacgcaa tggcttcatc acgagcttta
    23401 gcatcgcgga aagtgatgct caggccgtag ttgttttctt tacgaacggt ggtatacggg
    23461 atgccctttt cacgcaggtc gctgcgcagg ctatcgatat tttgttcctg gagtttgcca
    23521 agcgcggtgt ccatatccac ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg
    23581 agcttcatcg gctcagcatg aatggctgcc agccagcgcg gcgttgccgg ggcaagatta
    23641 agcgccacga cgtatttgtc acccataacg cccattaatg cttcacgagc gcgcagctgg
    23701 gtgtcagtgg agtcgaagcg cgcaagaata gcgccctctt ccagtgccac agacttagca
    23761 gttatttttt cttcttgtaa cgttttctgg acctggatca gcgtttgctc actggcggcg
    23821 actccgcgcg caccagtgat ctgaacagcc ggatcctcac caaacaggtt gggaagcgca
    23881 tacagcagac cgatgacaat caccacgatc agcatgacgt acttccacaa aggataacgg
    23941 tttaacacgg caattccctt agggaaaaat tttaattaca gcgccttcat ggtgcctttc
    24001 ggcagaacgg cagctacgaa gtcacgttta ataactactt cagtggtgtc attcagtgcg
    24061 atagcaatgt agccgttttc cgctactttg gttacgcgac cgaccaggcc accgttcgtc
    24121 agcacttcat cacctttagc gatggagtcc atcagctttt tgtgttcttt ggtgcgcttc
    24181 tgctgtggac gcaggatcat gaaatagaaa atcagaccga ataccaccag catcaaaatc
    24241 aaagacatcg ggctaccttg cgccggtgca cccgttgccg ctaccgcatc agaaataaaa
    24301 aagctcatta aatttccctc attattaata ttaatcaacg ttcaaaggtg gtacttctcg
    24361 cccctgacgc tggtaaaaat cagttacgaa gctctctaat ttaccctctt caatagcctt
    24421 gcgtaaaccc gccatcaaac gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc
    24481 gcctaatatt tcgttgcaac ggtcgagatg atgcaagtaa gcgcgtgaat aattgcgaca
    24541 ggtgtagcaa tcacactcag gatcgagtgg gccagtatcg ctcttatact tcgcattgcg
    24601 gattttcacc acgccatcgg tcacgaacaa atgaccattt cgggcgttgc gggttggcat
    24661 tacgcagtca aacatatcga taccgcgacg tacgccctca accaggtctt ctggtttacc
    24721 aacgcccatc aggtaacgcg gtttgtctgc cggaatttgc gggcatacat gctccagaat
    24781 gcggtgcata tctgctttcg gctcacccac agccagaccg ccgacagcgt agccatcaaa
    24841 accgatatct accagacctt taacagaaat atcacgtaaa tcttcgtaaa cactgccctg
    24901 aatgatacca aacagcgcat ttttgtttcc gagactgtca aaacgctcac ggctacgctt
    24961 cgcccaacgc agagacatct ccatggagag ttttgcgtaa tcccagtcag caggatacgg
    25021 cgtacactca tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa tctccatcga
    25081 cttttccggg tcgaggaaaa tcgggtcgcc gttgatcggg ttacggaagt gaacgccctg
    25141 ttcggtgatt ttacgaatat caccaaggct gaagacctgg aagccgccgg agtcggtaag
    25201 gattggtcct ttccactgca taaaatcgtg cagatcgccg tgcagtttca tgatttcctg
    25261 gcccgggcgc agccacaggt ggaaggtgtt gccgaggata atttgcgcgc cagtggcttc
    25321 aacttcttcc ggcgtcatcc cttttacggt gccgtaagtg ccaacaggca taaaacaagg
    25381 cgtttccact acgccacgat caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt
    25441 gtccagttca aatttcattt tttctccact acgtcagaaa aacagtccaa cgtttaaacc
    25501 agcgccgcgg aattactccc caacgcgctc attaattgcc tgcggattgt acgtgataaa
    25561 catcgcatca ccgtaactaa aaaagcgata tttctcttct accgccgctt tataggcatt
    25621 catggtgtgt tgataaccag caaatgccga aaccagcata atcagcgtcg attccggaag
    25681 gtggaagttc gtcaccagcg catcgaccac tttgtactgg aagcccggat agataaagat
    25741 ttgggtatcg tcaaagaacg gttcaatgag atcgtttttc gctgcctgag ccgcactttc
    25801 cagcgaacgt actgaggtgg taccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc
    25861 cagtaccgcg tctaccacat cctgcggcac ttcagcgtat tccgagtgca tgatgtgatc
    25921 ttcaatggtg tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa
    25981 cgccatctcc acgcctttgg cgcgtaattt ttccagcaaa ggttcgtcaa agtgcagtcc
    26041 ggcggtcggt gctgctaccg cgccgggttt ttcgctataa accgtttgat aaagttcgcg
    26101 gtctgcatct tcgtccggac ggtcgatgta cggcggcagc ggcatatggc cgatgctgtt
    26161 gagaatatcc agcaccgaac gttgatcatt aaattcgact tcaaacagtg cgccgtggcg
    26221 cgcggtcatt gttgcgttaa tactttcgtc atcgcccagc agcagttctg cgccaggttt
    26281 tggcgctttc gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca tccgctcaac
    26341 cagcacttca atcttgccgc cgctggcttt acgcccaaac agacgcgccg ggatcacgcg
    26401 ggtattatta aaaaccagaa gatcgccggg gttgagctta tcgagtaaat cggtgaaagt
    26461 accgtgcgtc agcgcgcccg tcggcccgtc cagcgacagt aaacgacagc tactgcgttc
    26521 aggcatggga tagtgggcaa tcagggattc gggcaattca aaagagaaat cggtaacgcg
    26581 catgacactg actcagacta aaataagagg cgggtagtct agtgccgggg cgctctccct
    26641 gcaaccttta cccccctttg gatagaatat aatcgatgaa ttttttagct cacctgcatt
    26701 tagcccatct cgcggaaagc tcgctttccg gcaatttact ggctgatttc gtacgcggaa
    26761 atcctgaaga aagttttccg cccgacgtcg tggctggcat tcatatgcat cggcgaatcg
    26821 acgtattaac cgacaatctg ccagaagtcc gcgaagcgcg ggagtggttt cgtaatgaaa
    26881 cgcgccgtgt cgcgcctatt acgctggatg tgatgtggga tcactttctg tcccgccact
    26941 ggtcgcagct gtcgcccgat tttccgctac aggaatttac ctgttatgcc cgggagcaag
    27001 tgatgactat tcttccagac tcaccgccac gctttatcaa tctgaacaac tacttatggt
    27061 cagagcggtg gttggtgcgc tatcgcgata tggatttcat ccagagcgtg ttaaacggca
    27121 tggcaagccg tcgcccacgt ctggatgccc tgcgtgactc ctggtacgat ttagacgccc
    27181 attacgatgc tctcgaaacc cgcttctggc agttttatcc gcggatgatg gagcaggcgt
    27241 cacgcaaggc gttataagac gttaattcat ccacaccgta gccgaaatgg caggcaaagc
    27301 cagaatcccg tccgtcagtt gcccgtgacc ttctttgcgt tgccattgcg cgacattaag
    27361 caacggcgac gcgggtagca ccacttcaca ggcttcgcca cggttgattg ccaccagtac
    27421 acgctgctga ttcagcacgc ggacaaatac caccacgtta tcttccgcat acagcacctg
    27481 acagccgcca cgacgcaacg cctgactttt cttacgcagc gcaatcattc gctggtacag
    27541 cgcgaataac gccgaatcct gcttttccac ctgccagggg aacggcttac ggcaaaacgg
    27601 agcgtttttg ccatccagtc ccacttcatc accgtaataa atgcacggta caccaggcca
    27661 ggtgaacagc cagaccaccg ccaggggcag gcgcgcaaca tcccgaccga gcagcgtttt
    27721 aaatcgcgca gtatcgtggc tgtcgagctg attaaacata cgtaattgtt gttgatgaga
    27781 aagccctgcg cggtaattat ccatccaggc catacaggtt tgggcgtcaa tttgctgcgg
    27841 atcgtaagag atatcggtgt tggcaagaaa tccccacaac gggaatgtga agccgcgata
    27901 gttcatggcg gcatcttcca catcggcctg taaccattgc cgtgcatcgc caaaatgttc
    27961 gccgacaata tacgcatccg gctgggtttc tttcgccgct tcggtgatcc cggcaacgtg
    28021 ctgcaaatta ttgcgcgccc cacccgcttc ccccagcata tgcaccacat ccagccgcca
    28081 gccatccata ttccacggcg ctttcagcca gtggcggaca atactgtctt ccccgcgata
    28141 aatttcattc accagacttt ccgactgata atccagcttc ggcaagctgg catagcccag
    28201 ccagtcgagc gccgtgccat catcactaaa cgagtaccag tcacgccagg gtgattcggg
    28261 gttgtgacaa gctccccccg tgccacgatt gtgcctgtca aaccaggcat gggaatcgcc
    28321 actgtggtta aacacgccgt ccagcaccag tcgaattccc agctgctgcg tattgtgtcg
    28381 caaacgcagc aacgccccat caccgccaaa ctgcggatcg acatggcgat aatcctcggt
    28441 atcgtattta tgcacgctgg gagctttaaa caccggattg agatacagcg ccgtcacgcc
    28501 aagttttttc agatacggca gtttttcgct tatcccgtcc agatcgccgc catagaacgt
    28561 tgatccgcca gcctgcgccg tgaccggttc atcccagtca cgcaatatga tctcttgtcc
    28621 ggctgcgtga tggtaataga catgatcctg ttcagcttca cgaggaagat tacgggcgaa
    28681 acgatcaggg aagatctgat aaaaaatctg atccgcagcc cattgtgggc cgatatccgg
    28741 tacatcgatg gcaaactgct ccagtcgtgc cggcggcatt cggctgaagc cttgcggtgt
    28801 aaaccaacgc tggcgatcgt gccacagcaa tttgaaacta taacgccgcc gaggttgtcc
    28861 gctggagaga tcgatcgccg cccgccatgc ggtgacgcca ggctgcggct gactgcgctg
    28921 cttatgcatc ggtactgaca tttcttcgtt atcgtgttct gtacgcagca taatgcgctg
    28981 cggtgggtct tcgcccgtca gccacagtgt aatgagcagt tgatctttgc tttgtttaac
    29041 aaatgggggc accggcagat gccatgcatt taacatcata attcccctgt gataaaaatc
    29101 tgatcacctt gccatagcct aatgcaggat cactcatcat ctgaaggata tttgggggag
    29161 gaggacgggg gcggataact ttcggaatag ccggatcagg atttcctcct gaaccggctt
    29221 acagaacgca aggattactg gttttcagcc agctgacgat cgtggcggcg cttaaacatc
    29281 cagccaatca acagcacaac aatccacgcg aaaccaacat acagcgagat acgcgtatca
    29341 gggtgataac caatcaaccc gataataaag agcaggaaaa tcaaaccgcc gatggtggtt
    29401 gcgaccccac ccggcacttt aaatttcagc gccttaactt cttctggcgg caaacggcga
    29461 cggaaggcga tttgcgacag caggatcata atccacaccc acaccgtggc gaaggttgcc
    29521 agcgaagcga tcaccaggaa gacgttttcc ggcatgatgt agttcagata caccgcaaac
    29581 agcagcgcgg tagtcatcac cagcaccgta acccacggaa taccacgacg cgaggttttg
    29641 ctgaaaattt ttggcgcgct gccctgctct gccataccgt ggagcatacg gcctacacca
    29701 aatacgtcac tgttaattgc cgacagcgaa gcggtcagca caacaaagtt aagaatgctg
    29761 gcggcaaagg taatgcccat atgctggaac gtcagcacaa acgggctacc ggcagtgcca
    29821 acctgattcc acgggtagat agacattatg acgaacagcg taccgacgta gaacaccaga
    29881 atacgcatcg gcacggagtt aatcgcacgc ggaatcgatt tctcaggatc tttcgcttca
    29941 ccggcggtaa tcccgataat ttcgatccca ccgtaagcaa acatcaccat ttgcaacgac
    30001 atcaccatac caagccagcc gttactgaag aagccgccgt tgctccacag gttatgaata
    30061 ccggtcggtt gcccgccgtt gccaatcccc cagatgatga tgccgaaacc ggcgacaatc
    30121 atgatgatga tagtggcgac tttaaagaac gagaaccaga actccagctc accgaatacc
    30181 ttcacgctca tcaggtttac ggcgcaaatg atcagcacca cactcagtac ccaaatccag
    30241 tgcggcaccg tcgggaacca gacacccata tagataccaa aagcggtcac atcggcgata
    30301 gcgacaataa ggatttcaaa gcagtaggtc cagccggtaa tgtaacctgc cagcgggccg
    30361 aggttttcct gcgcataacg cgagaaagag ctggctgccg ggttatgtac cgacatttcc
    30421 cccagcgcac gcataatgat atacgccgcg ataccaccga taatataggc caacaacacg
    30481 cttggaccgg ccattttgat ggcatctgcc gaaccgtaaa acagcccggt gccaattgct
    30541 gaacccagtg ccataaagcg tatgtgtcgg gtacttagcc cacgctttag cttgttctta
    30601 ctttccatca attcctaacc catcaacaca ataaaaaacc acggggcatc gcagccccgt
    30661 ggttaaaaca aatgttcagt gatttagtga gcgctggagg tcacctgacg acctgccgca
    30721 cgatcccaga taatggccag aaccaccatc accactgttg gcattaacca cgccagacct
    30781 tgttcggcca gcggtaaacg ctgcgcccag gacggtaaga tatcgctgaa tgcagatgcc
    30841 ttgatcccgt cgagaatacc aaaaagcagg ctgataaaca tcggcggagc aatcacgcgg
    30901 gacgaattat gccaccatga gcgtgtaaaa cttaatacaa ccagtgcgat acacggcgga
    30961 taaatggctg tcagcaccgg tacagagatc tgaatcagct ggctcaagcc gaggttagac
    31021 accaccatcg agaagccgcc gaggataaac accagcgtac gataagagag cggtacgtac
    31081 tgggcgaaga attctgcaca agcacaggtc aggccaaccg ccgtgaccag gcaggcgatg
    31141 aagattaacg ccgccagcag gaagctaccg ccgccgccaa aggtatgctg aacgtaagca
    31201 tgcaggatcg ccgcaccgtt tgcagactga tcgaccagcg acgcgctgtc tgaacccaga
    31261 cggaacagcg ccaggtacag cagagtcaga ccaacacccg ccatcaggcc agcccagacg
    31321 gtataacggg tcagcagacg cgcttcggta acgccacgag aacgcgccgc gttaacaata
    31381 acgataccaa acaccattgc gcccagcgta tccatggtca gatagccgtt gacgaagccg
    31441 ttagaaaacg cagcgttttg ataagcctca gtcgccgtac tgatagaacc cgccggccag
    31501 ataattgcgg caacagacag gatgaccagc gcgataattt tcagcggcgc aaggaagttg
    31561 cccacggtat ccagcagctt gcccggatag agcgaaacca gaataacgat agcgaaatag
    31621 accaggctgt aaataaacag cggcagcgcg gaatcacccg tcagcggcgc aatgcccact
    31681 tcaaaagaaa cggtagctgt acgcggcgta gcaaaaagcg gccccaccgc caggtaacaa
    31741 actgttgcca gcagtacgcc agcgacttta ccaattggcg tgctgagact gtcaacaccg
    31801 ccgccaactt ttgccagcgc cactaccgtt aataccggta ggccaacggc agtaatgagg
    31861 aagccgaatg ccgcagtcca gacgtgttcg cctgcctgca agccgaccat tggagggaaa
    31921 ataatgttac ctgcgccgac gaacaacgca aatgtcataa agcccagagc gatgatatcg
    31981 cgcgatctta attgatgggt cataaatcat actgcctgtg gatgtggtgt tgtaacgtta
    32041 aatttttgtg ccaatccctg gggatgatac agcggtatat ttaaacaatt ttagcgggaa
    32101 atactcccaa ccaggcgcgg cgaagaatag tttttcgatt taccgcgtaa atgcagtcag
    32161 tatgacagca cctgaagcgc aatttaaacg cttataacgt tttaaagcaa gaagggatct
    32221 aaaaaccaga ggtatatagc gataagaaat caccaaccag tataaaacac tatttcaatg
    32281 aaaaagacat ggctaatcat gcgaacaaaa aatcaccaac cgttgtaaaa tctaacgccc
    32341 gtatttcgac tgcaaagaaa ataagccagc atcggctggc ttatggcaat aaaagatgac
    32401 aaaggcgaat taatcgctgt ttttggcaat taaacgttcc gggataacaa aactgaaacg
    32461 tgtacctttt cctactgtac tctcaatatt caggcgactt tcgtgatgat tcaccgcatg
    32521 tttcacgatc gctaacccta atccgctacc accggtttgc cgggaacgcg ctttatcaac
    32581 gcgataaaaa cgctcggtca ggcgcggaat atgctccggt gcaatgcccg gtccgttatc
    32641 ttcaacacta aattcggcac cgtgcggcac tcgctgccag cgtacggtga tatgcgtgcc
    32701 ttccggcgta tgattcacgg cgttatggac caggttcgaa atcgcactgc gtagctgatc
    32761 ttcgttgcct gacaccttga ggccgttatc tatctcaaag gtaaatgtct gttttttctg
    32821 actcagagtc tgagcctcgc gctcaacgac gcgcagcatc atcggcacat caaccttttc
    32881 attgagcaaa tgcgtcggtg cggcttctat tttcgacagc gtcagcaatt gcttcaccaa
    32941 cccttccatc cgctgggtct gctcgcgcat ggtgtgcaac gctttttcac gtaccgcgcc
    33001 ttccagcggt tgctcatcca tcatctccag gtaaccctgt aacacggtca atggcgtacg
    33061 taactcatgg ctcacgttgg caaaaaagtt acgccgcgcc ccttccagtt gatgcatttg
    33121 cgtgacatct cgcgccacca tcagcaactg tttgtgggta taaggcatga cgcgaatctc
    33181 cagatgccgc ccggtgttga gcaccagatt gagcgggcga gagaaatcac tcgttttcag
    33241 atattgcgta aactccgggt aacgcagcaa gttaaggatg ttctgcccgt tatcttccgg
    33301 ccagcgcaag ccaagaatct gttgcgccag accgttgcac cagaaaatac cgccctcttc
    33361 cgtagtcagc accaccgcgt cgggcagcga ctccgcgccg ctacgaaagc gtttaatcag
    33421 attgcccagt tcacggcggc gttttttatt tcgtagctgc atctggtgta agccgtatag
    33481 tagcggttcc cagctaccac gccccggtgg cggggtcata ctacgatcca cccacagcca
    33541 ccatgaaagg cgcaataaat tccagaaatg ccagataagc agtcctgtta ccgatgccag
    33601 caaaaaccag ggcaggtaac caaaaaatgc acccaggatg aaagccggga ggcagcaaag
    33661 tagcagctcc agcaccagcc ttttccacga cagccgttcc agcacgtaag atactccagt
    33721 taagaaatca taagccctgc tctacgtccg atgagcaagg ccttaaaagc gggttgaaaa
    33781 acgataccct gtaccgcgca cggtctgcac catgcggtca tgcccgccgg gctccagtgc
    33841 tttacgcagg cgacgaatgt ggacatcgac cgtacgatct tccacataca cgttggttcc
    33901 ccagacgtgg tttaacagct gctcgcggct gtacacgcgc tcaggatgcg tcataaaaaa
    33961 gtgcagcagt ttaaattctg tcggccccat ctccagcggt tcttcgcccg ccatcacccg
    34021 gtgagatgtc gggtccagac ttagcccctg catctcgatc acctcttcca ccgccattgg
    34081 cgaaatacgg cgcattaccg ctttgattcg cgccaccagc tccttcggag aaaacggctt
    34141 ggtgatatag tcatccgcac cggtttcaag gccgcgcacg cgatcttctt cttcccctct
    34201 ggcggtcaac atcaccactg gaatatcccg ggtcatcgac tcgcgcttga ggtgtttgat
    34261 gaactggata ccggagccgc caggtaacat ccagtcgagg agaattaaat ccggccaggg
    34321 ttcattcagt tgattcacag cactgtcata atcttccgct tcgaccggct gaaagccatt
    34381 ttgttcgagc acgaagcaga ccatttcgcg aattggagct tcatcttcta cgaccagaat
    34441 acgtctcgcc atgatttgcc ctgttgtaat aaataggttg cgatcattaa tgcgacgtca
    34501 ttatgcgtca gatttatgac agatttatga aaagctcgtc gcacatcctt tcaggttatt
    34561 gatttccgtg gcacagaaaa aagcaaatgg cacatctgtt tgggtataat cgcgcccatg
    34621 ctttttcgcc agggaaccgt tatgcgcatc cttcacacct cagactggca tctcggccag
    34681 aacttctaca gtaaaagccg cgaagctgaa catcaggctt ttcttgactg gctgctggag
    34741 acagcacaag cctatcaggt ggatgcgatt attgttgccg gtgatgtttt cgataccggc
    34801 tcgccgccca gttacgcccg cacgttatac aaccgttttg ttgtcaattt acagcaaacg
    34861 ggctgtcatc tggtggtact ggcaggaaac catgactcgg tcgccacgct gaatgaatcg
    34921 cgcgatatca tggcgttcct caatactacc gtggtcgcca gcgccggaca tgcgccgcaa
    34981 atattgcccc gtcgcgacgg gacgccaggc gcagtgctgt gtcccattcc ttttttacgc
    35041 ccgcgtgaca ttattaccag ccaggcgggg cttaacggta ttgaaaaaca gcagcatttg
    35101 ctggcggcta ttactgatta ttatcaacaa cattatgccg atgcctgcaa actgcgcggc
    35161 gatcagcctc tgcccatcat cgccactgga catttaacga ccgtgggtgc cagtaaaagt
    35221 gacgccgtgc gtgacattta tattggcacg ctggacgcgt ttccggcaca aaactttcca
    35281 ccagccgact acatcgcgct cgggcatatt caccgcgcgc agattattgg cggcatggaa
    35341 catgttcgct attgcggttc ccccatacca ctgagttttg atgaatgcgg taagagtaaa
    35401 tatgtccatc tggtgacatt ttcaaacggc aaattagaga gcgtggaaaa cctgaacgta
    35461 ccggtaacgc aacccatggc ggtgctgaaa ggcgatctgg cgtcgattac cgcacagctg
    35521 gaacagtggc gcgatgtatc acaggagccg cctgtctggc tggatatcga aatcactact
    35581 gatgagtatc tgcatgatat tcagcgcaaa atccaggcat taaccgaatc attgcctgtc
    35641 gaagtattgc tggtacgccg gagtcgtgaa cagcgcgagc gtgtgttagc cagccaacag
    35701 cgtgaaaccc tcagcgaact cagcgtcgaa gaggtgttca atcgccgtct ggcactggaa
    35761 gaactggatg aatcacagca gcaacgtctg cagcatcttt tcgccacgac gttgcatagc
    35821 ctcgccggag aacacgaagc atgaaaattc tcagcctgcg cctgaaaaac ctgaactcat
    35881 taaaaggcga atggaagatt gatttcaccc gcgagccgtt cgccagcaac gggctgtttg
    35941 ctattaccgg cccaaccggt gcggggaaaa ccaccctgct ggacgccatt tgtctggcgc
    36001 tgtatcacga aaccccgcgc ctctctaacg tttcacaatc gcaaaatgat ctcatgaccc
    36061 gcgataccgc cgaatgtctg gcggaagtgg agtttgaagt caaaggtgaa gcgtatcgcg
    36121 cgttctgggg ccagaatcgg gcgcgtaacc agccggacgg taatttgcag gtgccacgcg
    36181 tagagctggc gcgctgtgca gacggcaaaa ttctcgccga caaagtgaaa gataagctgg
    36241 aactgacggc gacgttaacc ggactggatt acgggcgctt cacccgttcg atgctgcttt
    36301 cgcaggggca atttgctgcc ttcctgaatg ccaaacccaa agaacgcgca gaattgctcg
    36361 aggagttaac cggcactgaa atctacgggc aaatctcggc gatggttttt gagcagcaca
    36421 aatcggcccg cacagagctg gagaagctgc aagcgcaggc cagcggcgtc gcgttgctca
    36481 cgccggaaca agtgcaatcg ctgacagcga gtttgcaggt acttactgac gaagaaaaac
    36541 agttaattac cgcgcagcag caagaacaac aatcgctaaa ctggttaacg cgtctggacg
    36601 aattgcagca agaagccagc cgccgtcaac aggccttgca acaggcatta gccgaagaag
    36661 aaaaagcgca acctcaactg gcggcgctca gtctggcaca accggcacga aatcttcgtc
    36721 cgcactggga acgcatcgca gaacacagca cggcgctggc gcatactcgc cagcagattg
    36781 aagaagtaaa tactcgctta cagagcacaa tggcgcttcg tgcgagcatt cgccaccacg
    36841 cggcgaagca gtcagcagaa ttacagcagc agcaacaaag cctgaatgcc tggttacagg
    36901 aacacgaccg cttccgtcag tggaacaacg aactggcggg ttggcgtgca cagttctccc
    36961 aacaaaccag cgatcgcgag catctgcggc aatggcagca acagttaact catgctgagc
    37021 aaaaacttaa tgcgcttgcg gcgatcacgt tgacgttaac cgccgatgaa gttgctagcg
    37081 ccctggcgca acatgctgag caacgcccac tgcgtcagcg tctggtcgca ctgcatgggc
    37141 agattgttcc ccaacaaaaa cgtctggcgc agttacaggt cgctatccag aatgtcactc
    37201 tggaacagac gcaacgtaat gccgcactta acgaaatgcg ccatcgttat aaagaaaaaa
    37261 cgcaacaact tgccgatgtg aaaaccattt gcgagcagga agcgcgcatt aaaacgctgg
    37321 aagcccagcg cgcacagtta caggcgggtc agccttgtcc actttgtggt tccagcagcc
    37381 acccggcggt cgaggcgtat caggcgctgg aaccgggcgt taatcaggcc cggctattaa
    37441 cgctggaaaa agaagtgaaa aagctcggcg aagaaggtgc ggcgctacgt gggcaactgg
    37501 atgcattaac gaagcagctt cagcgcgatg aaaacgaagc gcaaagcctc cgacaagatg
    37561 agcaagcact tactcaacaa tggcaagccg tcacggccag cctcaatatc accctgcagc
    37621 cacaggacga tattcaaccg tggctagatg cacaagatga gcacgaacgc cagctgcggt
    37681 tactcagcca acggcatgaa ttacaagggc agattgccgc gcataatcag caaattatcc
    37741 agtatcaaca gcaaattgaa caacgccagc agcaactctt aacggcattg gcgggttatg
    37801 cgctgacatt gccacaggaa gatgaagaag agagctggtt ggcgacgcgt cagcaagaag
    37861 cgcagagctg gcagcaacgc cagaacgaat taaccgcgct gcaaaaccgt atgcagcagc
    37921 tgacgccgat tctggaaacg ttgccgcaaa gtgatgaact cccacacagc gaagaaactg
    37981 tggcgctgga aaactggcgg caggttcatg aacaatgtct cgcattacac agccagcagc
    38041 agacgttaca gcaacaggat gttctggcgg cgcaaagtct gcaaaaagcc ctggcgcagt
    38101 ttgacaccgc gctacaggcg agcgtctttg acgatcagca ggcgttcctt gcggcgctaa
    38161 tggatgaaca aacactaacg cagctggaac agctcaagca gaatctggaa aaccagcgcc
    38221 gtcaggcgca aacgctggtc actcagacag cagaaacgct gacacagcat cagcaacacc
    38281 gacctggcgg gttatctctc actgtgacgg tggagcagat tcagcaagag ttagcgcaaa
    38341 ctcaccaaaa gttgcgtgaa aacaccacga gtcaaggcga gattcgccag cagctgaagc
    38401 aggatgcaga taaccgtcag caacaacaaa ccttaatgca gcaaattgct caaatgacgc
    38461 agcaggttga ggactgggga tatctgaatt cgctaatagg ttccaaagag ggcgataaat
    38521 tccgcaagtt tgcccaaggg ctgacgctgg ataatttagt ccatctcgct aatcagcaac
    38581 ttacccggct gcacgggcgc tatctgttac agcgcaaagc cagcgaagcg ctggaagttg
    38641 aggttgttga tacctggcag gctgatgcgg tacgcgatac ccgtaccctt tccggcggcg
    38701 aaagtttcct cgtcagtctg gcgctggcac tggcgctttc agatctggtc agccataaaa
    38761 cgcgtattga ctcgctgttc cttgatgaag gttttggcac actggatagc gaaacgctgg
    38821 ataccgccct tgatgcgctg gatgccctga atgccagtgg caaaaccatc ggggtgatta
    38881 gccacgttga ggcgatgaaa gagcgtattc cggtgcagat caaagtgaag aagatcaatg
    38941 gactgggcta cagcaaactg gaaagtgcgt ttgcaatgaa ataactattc agcaggataa
    39001 tgaatacaga ggggcgaatt atctcttggc ctcgctggtc gttatcctgc aatctatcac
    39061 tttattggct acggtgattg gtagccgttc tggtggttgt gatggtggta tgaaaaaagt
    39121 tattttatct ttggctctgg gcacgtttgg tttggggatg gccgaatttg gcattatggg
    39181 cgtgctcacg gagctggcgc ataacgtagg aatttcgatt cctgccgccg ggcatatgat
    39241 ctcgtattat gcgctggggg tggtggtcgg tgcgcctata atcgcgcttt tttccagccg
    39301 ctactcactc aaacatatct tgttgtttct ggtggcgttg tgcgtcattg gcaacgccat
    39361 gttcacgctc tcttcgtctt acctgatgct cgccattggt cggctggttt ccggctttcc
    39421 gcatggtgca ttttttggcg tcggcgcgat cgtgttatca aaaattatca aacccggcaa
    39481 agtcaccgcc gccgtggcag ggatggtttc cgggatgaca gtcgccaatt tgctgggcat
    39541 tccactggga acgtatttaa gtcaggaatt tagctggcgc tacacctttt tgttgatcgc
    39601 tgtttttaat attgcggtga tggcatcggt ctatttttgg gtgccggata ttcgcgacga
    39661 ggcgaaagga aaactacgcg aacaatttca ctttttacgc agccctgccc cgtggttaat
    39721 tttcgccgct acgatgtttg gcaacgcagg tgtgtttgcc tggttcagct acgtaaagcc
    39781 atacatgatg tttatttccg gtttttcgga aacggcgatg acctttatta tgatgttagt
    39841 tgggctaggg atggtgctgg ggaatatgct aagtggcagg atttcaggac gttattcacc
    39901 actgcgcatt gcagcagtga ctgactttat cattgtactg gcactgctga tgctcttttt
    39961 cttcggcggt atgaaaacaa cgtcgcttat ttttgctttt atttgttgcg cgggattatt
    40021 tgccctatca gcacctctac aaatattatt actgcaaaat gccaaaggcg gagagttatt
    40081 aggtgccgca ggtgggcaaa tagcgtttaa cctcggtagc gccgtcggcg catattgcgg
    40141 tggtatgatg ctgacgctgg ggctggcata taattacgtg gcgctgcccg ctgccctgct
    40201 ttcgtttgct gcgatgtcgt cgttgctact gtatggtcgc tataagcgcc agcaagcggc
    40261 ggatagttcg gtgctggcga aaccactggg gtagatagca gtcgcagtgc gcatcgcttg
    40321 ttgccggatg cggcgtgaac gccttatccg gcctacggga ctgttcattt tgtaggcctg
    40381 ataagacacg ccagtgtcgc atcaggcatc ggagcactgg ggggttgccg ccactgtgcg
    40441 cgttatttgt tgtcggatgc ggcgcgagcg ccttatccgg cctaccggac ggctcacttt
    40501 gtaggcctga taagacgcgg caagcgtcgc atcaggcatg atacgccaat tgcctggttt
    40561 ttttactctt gtggccataa ccatgcagcg ccgcgtacgc cgctcgaatc accatgtttc
    40621 gccttgcgta ccggcgtttc acattctccg ccgaagacaa attgtttaat caactgtgga
    40681 accgtttgat ataaacggtc tacattgctc attccgccgc ccaggacaat cacatccgga
    40741 tcgagaatat tcacgacatg tgccagcgat tttgccagcc gcagctcgta gcgacgcaat
    40801 gccagttccg ctaccggatc gctttcttca accaggcgga taatttcact gcctttcagc
    40861 gcatgtccgc tcaaacgacg ataatccgtc gcgaatcccg tgcctgaaat aaaggtttca
    40921 atacaacctt gtttaccgca ataacaaggg acttcctcgc gataacgcag ttcgtcttcg
    40981 tccatccacg gtagcggatt gtgtccccac tcgcctgccg tgccattgcc gccgatatgc
    41041 gcccgcccat tgaatgccac gcccgcgccg catcccgtgc cgataatcac ggcaaatacc
    41101 gtctgcgctc ccgctgccgc gccatctacc gcttctgaaa ccgccagaca gttggcgtca
    41161 tttgccagcc gcacttcccg ctgcaaccgc gcgcttaagt ctttatcgaa tggctgaccg
    41221 ttgagccagg ttgaattggc attcttcacc acgccggtgt agggcgaaat cgaaccagga
    41281 atgcccatac ctaccgttcc gcgctgccct gttgcctgct ccgccatatc aaccaacgtg
    41341 gcgatcgttt caatagtctg ctggtaatca tcacgcggcg tgggcagacg atggcggtac
    41401 aactgctccc ctgcatcgcc caatgcaatc acttcagttt tggtgccgcc taaatcgata
    41461 cctatacgca cggtactctc cttatttttt tcaatatcaa tagcgtagag acggacaacc
    41521 ggattggcaa tgcaaggccg ccgacaattc gttatcatgc ccgctaaatt taacgacaag
    41581 gccgtggaaa ttatcatgct gtggttcaaa aatttaatgg tttaccgtct tagccgcgag
    41641 atttcgctgc gtgcagaaga gatggaaaaa cagctagcct cgatggcatt taccccatgc
    41701 ggcagccagg acatggcgaa gatgggctgg gtacctccga tgggatcgca cagcgatgcg
    41761 ttaacgcacg ttgccaatgg tcaaattgtt atctgtgcgc gcaaagaaga aaaaatcctc
    41821 ccgtccccgg tgattaaaca ggcgctggaa gcgaaaatcg ccaaactgga agcagaacag
    41881 gcgcgtaagc tgaagaaaac cgaaaaagat tcgctgaaag acgaagtgct gcactctctg
    41941 ctgccgcgtg ctttcagccg ttttagccag acaatgatgt ggatcgacac ggttaacggt
    42001 ttgattatgg tggactgcgc cagcgccaaa aaagcggaag atacgctggc actgctgcgt
    42061 aaaagcctgg ggtcgttacc ggttgtacca ttgagcatgg aaaacccgat tgaactgacg
    42121 ctgaccgaat gggtacgttc cggcagcgcg gcacagggct tccagctgct tgatgaagcc
    42181 gagctgaaat cgttgctgga agatggcggc gtgatccgcg cgaagaaaca agatctgacc
    42241 agcgaagaga tcaccaacca cattgaagcc ggaaaagtgg tgactaaact ggcgcttgac
    42301 tggcagcagc gcattcagtt tgtgatgtgc gacgatggtt cgctcaagcg tctgaagttc
    42361 tgcgacgagc tgcgcgatca aaacgaagat atcgaccgtg aagatttcgc ccagcgcttt
    42421 gatgccgatt tcatcctgat gactggtgaa ctggcagcgt taattcaaaa cctgattgaa
    42481 ggattagctg gcgaagcaca acgttaattg ctgatttacc tttaatgccg gatgcgacgc
    42541 ctgccgcgtc ttatccggcg tacaaagcca caccaggaat ataatcgtta gctacagcgg
    42601 gcaataattc ttagcgcagc aatattatgc gttttatgct gtaacttgct ccaggggcgt
    42661 tgtgtcattg tttttcctca agccggtgac gcgcttcact gcgaattttc cgcgttgccc
    42721 cgtttttcac taaatcacgt aacagcgaaa aatcgggttg ttcgacaaat aagagtaatg
    42781 atggctcctc ttttaaccac gtcgttttca catccgcagc cagctgtgaa ttattaatag
    42841 ccagcgaccg ctcctgaggc tcagtcaatg tcgttaacaa caccggagta tgtgcgttgt
    42901 gcaatgccgt ttggcgcaaa tgatcattgc gactttgcag caatgcacac caccattcat
    42961 catctgattt atcttttaac gtcgccagtt ccgcaacaat atctggagat cttcgctgat
    43021 atttatatca accttgatga attatgaata tatcggaaat cgaaaaaatc ccagcaaagg
    43081 aaatattatt atgggttaaa gagaggacat ttcagaaaaa tgccctcccg ttcaagggga
    43141 aggcgaggaa ttacagatag cggcacagat aagaggtggg ttcggcaact tgcagatgaa
    43201 actcgctgtg accgggaaca ttaaacaccg aaccggcttc atacacttgc cagtcggtcg
    43261 cgtcaggcag taacacattc agcgcgccac tgattaccgt catctcttca ggctcagcgg
    43321 tgctgaaggt gtattcacct tcaaccataa cacccacgct ggcgcgacca gtgctgctgc
    43381 tggaaaagcc gatcgatttc actttgccgg aaaagtactc attactttga agcataaact
    43441 ggccctgtct ggtagttgaa catctacttc actatagggg ccagaggcgc tgactgtcac
    43501 gcaaaattac atcagtaatt ccgcagccag ccgtgcaatc aatacgttag agagcaagac
    43561 aggaacatcg agctgttttt gcagtaaatc gcgatgacgc tggttaaatc ccaaacaatc
    43621 cagcatgatg acatctgcac cttttgccag taattctttc ccggcatcaa tgattttttg
    43681 ttccgaatca tgaatggggt tacccaatga aaatactggc ggtttctgca aaatttgcca
    43741 tttttgcgcc tgaacggtca gcaactcctc aaccggtacg ataaccccca cctgatgatc
    43801 ttcaacaata gaggaaacca gcggtggtaa tatccgcaac ggctcaagaa agatcgtatt
    43861 acgcgcggtc atactactaa tgtttgccgt actcattaat ataatgacgt cgtaaccttg
    43921 attatcgagc acttcaacca caccttgcag gtctcgctcc actttgcgac gcgaaacatg
    43981 ggccagctgg ttgtcattta ataatgtgag aattgtgtct tcgcctgctt ctggcgcgta
    44041 ctccgccatc acttcttcgc ggcttaactt ccccagcagg ctatgatggg aaatattatc
    44101 ttcgtcaatg tattctgtca ggagcggcaa aacttcctgc ataggtacaa tgccgatagt
    44161 caggatcgcc aacgacgcac tcattgttct tccaccttac ttttatttcg tcgttaatta
    44221 ccgggcaagt gttaagcacc actgctgaca cttacttgtg ctacaaatga caaaaagcgt
    44281 agcagcagac ctgcactgca taatgtaaag attccaaatg attccagtaa tggattgtta
    44341 ttgtttaata ttctaattat ttgaaaaaca tgaattgtga aaaatgtgac gcagatccct
    44401 tgtggcggca ctcagaatcc cttccgagca gtctgatgcg gtcactcttt gtcagggtcc
    44461 tcatagcgtt ttttcgcatc catcgcttcc tgagcggtcg gatgttcact gatcaacgag
    44521 tctggtttag gatgatcggc tctgagttgg taccaggtta ccgtctgtcc tggctttcct
    44581 ttttcaatcg tcactatata tgcttcacga ggataaggcg gtttcgttgg catcgttctt
    44641 ccttatttca cgggatgaac gttaagtata ggcgctcgaa aatcaacaat tgatcgtctg
    44701 tgccagagcg ctgcgaattt cagaaatcac ctggctgggt tcgtttgttg cgtcgatgat
    44761 aatatgcgca acttcgcgat atagcgcatc gcgttcttcc agcacttcct gaacttcttc
    44821 gctcagtggt tttcccgtta aggttggccg taaatcttct tccggtgcag cttgcagtcg
    44881 gttaaccagg actgatactg gcgcacacaa ataaaccacg atcccgttat tttgcatgaa
    44941 gtgacgatta aattccgtca gaataatgcc gccgcccgtg gcgataacgg tggatggcgc
    45001 agttaccgct tccagcgccg ccgtttctct ggcgcggaat cccgcccact cttccctttc
    45061 gacgatgtcc gcgaccgtca tattgagctg tgattgcaac cactggtcgg tatcgacaaa
    45121 ccgacggtta agcgaatcgg caagggccat tccgaccgtt gttttaccac agccccgagg
    45181 cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc caataggtcg caatgcggcg
    45241 aaagccggtg tcatgagaat agcgatcata ccatcaaact agtacaattt cgattgtaaa
    45301 gaaaaaattc cacttaaggt ggaaatctca atacatccct tacta
//
LOCUS       DADTFG010000015        44882 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.015, whole genome shotgun sequence.
ACCESSION   DADTFG010000015 DADTFG010000000
VERSION     DADTFG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.015"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(111..187)
                     /locus_tag="J1138_000873"
     tRNA            complement(111..187)
                     /locus_tag="J1138_000873"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Met,seq:cat)
     gene            396..1493
                     /gene="mltA"
                     /locus_tag="J1138_000874"
     CDS             396..1493
                     /gene="mltA"
                     /locus_tag="J1138_000874"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBA8452710.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1570..2376
                     /gene="tcdA"
                     /locus_tag="J1138_000875"
     CDS             1570..2376
                     /gene="tcdA"
                     /locus_tag="J1138_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBA8452711.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2427..2870)
                     /gene="csdE"
                     /locus_tag="J1138_000876"
     CDS             complement(2427..2870)
                     /gene="csdE"
                     /locus_tag="J1138_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBA8452712.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2870..4075)
                     /gene="csdA"
                     /locus_tag="J1138_000877"
     CDS             complement(2870..4075)
                     /gene="csdA"
                     /locus_tag="J1138_000877"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBA8452713.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4267..4494
                     /locus_tag="J1138_000878"
     CDS             4267..4494
                     /locus_tag="J1138_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBA8452714.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4846..5763
                     /locus_tag="J1138_000879"
     CDS             4846..5763
                     /locus_tag="J1138_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HBA8452715.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKIPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5782..6177
                     /locus_tag="J1138_000880"
     CDS             5782..6177
                     /locus_tag="J1138_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBA8452716.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6170..7270
                     /gene="rlmM"
                     /locus_tag="J1138_000881"
     CDS             6170..7270
                     /gene="rlmM"
                     /locus_tag="J1138_000881"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBA8452717.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7314..8045)
                     /gene="fucR"
                     /locus_tag="J1138_000882"
     CDS             complement(7314..8045)
                     /gene="fucR"
                     /locus_tag="J1138_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBA8452718.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(8103..8525)
                     /gene="fucU"
                     /locus_tag="J1138_000883"
     CDS             complement(8103..8525)
                     /gene="fucU"
                     /locus_tag="J1138_000883"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBA8452719.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8527..9945)
                     /gene="fucK"
                     /locus_tag="J1138_000884"
     CDS             complement(8527..9945)
                     /gene="fucK"
                     /locus_tag="J1138_000884"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBA8452720.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10054..11829)
                     /gene="fucI"
                     /locus_tag="J1138_000885"
     CDS             complement(10054..11829)
                     /gene="fucI"
                     /locus_tag="J1138_000885"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBA8452721.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11862..13178)
                     /gene="fucP"
                     /locus_tag="J1138_000886"
     CDS             complement(11862..13178)
                     /gene="fucP"
                     /locus_tag="J1138_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBA8452722.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13725..14372
                     /gene="fucA"
                     /locus_tag="J1138_000887"
     CDS             13725..14372
                     /gene="fucA"
                     /locus_tag="J1138_000887"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBA8452723.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14400..15548
                     /gene="fucO"
                     /locus_tag="J1138_000888"
     CDS             14400..15548
                     /gene="fucO"
                     /locus_tag="J1138_000888"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBA8452724.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVIDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGTVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15653..16498)
                     /gene="xni"
                     /locus_tag="J1138_000889"
     CDS             complement(15653..16498)
                     /gene="xni"
                     /locus_tag="J1138_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBA8452725.1"
                     /translation="MRGLFPIPHPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHNEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16520..17887)
                     /gene="sdaB"
                     /locus_tag="J1138_000890"
     CDS             complement(16520..17887)
                     /gene="sdaB"
                     /locus_tag="J1138_000890"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBA8452726.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17945..19234)
                     /locus_tag="J1138_000891"
     CDS             complement(17945..19234)
                     /locus_tag="J1138_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA8452727.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19791..21155)
                     /gene="ppnN"
                     /locus_tag="J1138_000892"
     CDS             complement(19791..21155)
                     /gene="ppnN"
                     /locus_tag="J1138_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBA8452728.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21267..22115)
                     /gene="queF"
                     /locus_tag="J1138_000893"
     CDS             complement(21267..22115)
                     /gene="queF"
                     /locus_tag="J1138_000893"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBA8452729.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22183..22728
                     /gene="syd"
                     /locus_tag="J1138_000894"
     CDS             22183..22728
                     /gene="syd"
                     /locus_tag="J1138_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBA8452730.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAIYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23350..23679
                     /locus_tag="J1138_000895"
     CDS             23350..23679
                     /locus_tag="J1138_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBA8452731.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23679..24461
                     /gene="truC"
                     /locus_tag="J1138_000896"
     CDS             23679..24461
                     /gene="truC"
                     /locus_tag="J1138_000896"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBA8452732.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24479..24928
                     /locus_tag="J1138_000897"
     CDS             24479..24928
                     /locus_tag="J1138_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBA8452733.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            25362..26714
                     /locus_tag="J1138_000898"
     CDS             25362..26714
                     /locus_tag="J1138_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452734.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            26716..28056
                     /locus_tag="J1138_000899"
     CDS             26716..28056
                     /locus_tag="J1138_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase-related protein"
                     /protein_id="HBA8452735.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            28077..29417
                     /gene="gudD"
                     /locus_tag="J1138_000900"
     CDS             28077..29417
                     /gene="gudD"
                     /locus_tag="J1138_000900"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBA8452736.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29648..32404)
                     /gene="barA"
                     /locus_tag="J1138_000901"
     CDS             complement(29648..32404)
                     /gene="barA"
                     /locus_tag="J1138_000901"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBA8452737.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32461..33762
                     /gene="rlmD"
                     /locus_tag="J1138_000902"
     CDS             32461..33762
                     /gene="rlmD"
                     /locus_tag="J1138_000902"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBA8452738.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDCVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33810..36044
                     /gene="relA"
                     /locus_tag="J1138_000903"
     CDS             33810..36044
                     /gene="relA"
                     /locus_tag="J1138_000903"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HBA8452739.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRDSVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            36140..36931
                     /gene="mazG"
                     /locus_tag="J1138_000904"
     CDS             36140..36931
                     /gene="mazG"
                     /locus_tag="J1138_000904"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBA8452740.1"
                     /translation="MNQIDRLLTVMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37159..38796
                     /gene="pyrG"
                     /locus_tag="J1138_000905"
     CDS             37159..38796
                     /gene="pyrG"
                     /locus_tag="J1138_000905"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBA8452741.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            38884..40182
                     /gene="eno"
                     /locus_tag="J1138_000906"
     CDS             38884..40182
                     /gene="eno"
                     /locus_tag="J1138_000906"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBA8452742.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40242..41144)
                     /locus_tag="J1138_000907"
     CDS             complement(40242..41144)
                     /locus_tag="J1138_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBA8452743.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVVFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     GGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(41141..42289)
                     /locus_tag="J1138_000908"
     CDS             complement(41141..42289)
                     /locus_tag="J1138_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBA8452744.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQSALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQLSLPCLAWTVLMIVAIIVLWRFTSRPGPGIWMISMITPTVWFFCFQ
                     DDVIIRRVSVVCFSLFFAAICWQRLAVVFNMCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            complement(42304..43197)
                     /locus_tag="J1138_000909"
     CDS             complement(42304..43197)
                     /locus_tag="J1138_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBA8452745.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPRYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNHAQLVVLVVDTTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGINDIESRLMGNNSA
                     TITPTDHPLPFSGWWALLVWAIILTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLTTLLCFATPFLAVAIAFTPFGKKVRDSMRDANQPSRHTRSNSSSHYSSSSSSSF
                     DNDNFSGGGGSSGGGGASGRW"
     gene            complement(43211..43813)
                     /locus_tag="J1138_000910"
     CDS             complement(43211..43813)
                     /locus_tag="J1138_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HBA8452746.1"
                     /translation="MFRIFIAFIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRT
                     DLIPNLVASIKGYSSHEQEVLEAVTLARSQANRASSDLQKTPGDEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPQLKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVTAVSKAPTIDFSQNANAH"
     gene            43986..44657
                     /gene="queE"
                     /locus_tag="J1138_000911"
     CDS             43986..44657
                     /gene="queE"
                     /locus_tag="J1138_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBA8452747.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT        11172 a        10856 c        11757 g        11097 t
ORIGIN      
        1 ttaacggttt actcattgtc tcaggcttca ctgaaacggg taaggaagcg taaaaacgaa
       61 aaaaccgcaa gccaatagta gctttgcggt ttgtcagcat tctaataaat tggttgcggg
      121 ggccggattt gaaccgacga ccttcgggtt atgagcccga cgagctacca ggctgctcca
      181 ccccgcgtcc gtggatgcgc actatactct gctcgcgttt tcatgcaacc ttttttttac
      241 cccgatcacg gatttggcat gattttgaac aaaaacaaat cactaaagcc tattttttgt
      301 gcaaaatttg cgtcagggag aggctgctca tttcattaaa cgcggtggtt tgttatcttc
      361 gttgcgcctt attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc
      421 ttcttatggg cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg
      481 gacagcaata taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg
      541 ccgttggcgc gccgattaat gccggtgatt ttgccgagca aattaaccat atccgtaatt
      601 cgtcaccgcg tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc
      661 gcgcaggcgg tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag
      721 gtgtcgacaa ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc
      781 gccatacccg tcagggcgag ttccagtatc cgatttatcg tatgccgcca aaacgtggtc
      841 gtctgccgtc tcgtgcggag atctacgcag gagcgctgag tgataaatat attctcgctt
      901 acagtaactc cctgatggat aacttcatta tggatgtgca gggtagcggg tatatcgact
      961 ttggtgatgg cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc
     1021 gcagcattgg taaggtgttg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc
     1081 aggcgattcg tcactggggc gaaacacaca gtgaagcgga ggttcgcgag ctgctggaac
     1141 agaacccgtc tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg
     1201 cggtgccgct ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta
     1261 ctaccttgct ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg
     1321 aattgcgtct gatggtggcg ctggatgtcg gtggcgcaat caaaggccaa cacttcgata
     1381 tctatcaagg gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac
     1441 gtgtctgggt gctgaaaacc gccccgggcg caggtaacgt ctttagcggc tgatgtgata
     1501 ttctgtatgc cgtttttacg gatatgacag ggtgaggggt aacctcaccc tttttaattc
     1561 tgaggtgcta tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg
     1621 cgtctgtatg gtgaaaaagc attgcaactg tttgctgacg cgcatatttg tgtggttggt
     1681 atcggtggtg tcggttcctg ggcggcggaa gcgctagcgc gcacggggat tggcgcaatc
     1741 acgcttatcg atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg
     1801 cgcgataacg tcgggctggc aaaagcggaa gtaatggcgg agcgtattcg ccagattaac
     1861 ccggagtgcc gtgtaacagt agtggatgac ttcgtgacgc cggataacgt agcgcaatat
     1921 atgaatgccg gttattcgta cgtgattgat gctattgata gtgtacggcc caaagcggcg
     1981 ctgattgctt attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgctggtggg
     2041 cagattgacc cgacgcagat tcaggttacc gatctggcga aaacgattca ggacccgttg
     2101 gcggcgaagt tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt
     2161 aagctcggcg tggattgcgt gttctctacc gaagcgctgg tgtacccgca gtcagacggc
     2221 accgtctgtg cgatgaaagc cacggcggaa gggccaaagc ggatggattg tgcctcagga
     2281 tttggcgcgg cgacgatggt gaccgccact tttggctttg ttgcggtttc tcatgcgctg
     2341 aagaagatga tggcgaaagc ggcgcgtcag gggtaaacaa tcaggtcgga tgcgacgcga
     2401 acgtcttatc cgacctggtt ctgtgcttaa acctgcttcg ccgcagcgat aatcgcctcg
     2461 cttaacgcat tcaacccctg gctgcgtgag gcgctaagtt gcgcacgtaa tcccagctca
     2521 tcaaacaatg ccagcggcga ctgtgcctgc aattcggcgg cggttttccc ctcaacggca
     2581 gtcaacaaca ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaaatgc
     2641 atcttgccgt tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc
     2701 tctttagcct gcgcttttaa ctcctctggc aaagccggaa gctgtttccc cagcatgatc
     2761 aactggcgat atttatcttc ccattgcgtc aacggtgcga aggtattgcg taacgtttct
     2821 gcggttacgg ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca
     2881 ataattccag cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat
     2941 atggcgcaaa tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag
     3001 cgcaatgctg cccggcgcgc agggcaatac cgtactccgc cagcagtgtc accatatcgc
     3061 tgtgatgaac tccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac
     3121 gaaagcctgg acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc
     3181 tttcggcctg gttgatatcg taatctgtca gccattccag cgccgcgctt aatcctatga
     3241 caccagcgac atttggcgtt cctgcttcca gtttccacgg cgcagattga gtcgtgaagc
     3301 cgtcaaaact cacttcgtga accattttgc cgccgcccag ccagggcgac atcgcttcca
     3361 gcagttctgg tttgccatac agcacgccga tgcctgtcgg gccatacagt ttgtgacctg
     3421 aaaaagcata gaaatcaata tccagttgct gaacatccgc ggggaaatgc accgccccct
     3481 gagcaccatc aaccatcacc actatcccgg ctgaatgagc aaaagtaatc gctctcgcca
     3541 gatccgggca accgccagtg acgttcgaca tctggcccaa cgccagaatc cgactacggg
     3601 gagtaatcag ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt
     3661 tcaccacttt ggcccccgtt tgttgggcga ccatcagcca ggggacgagg ttggcgtggt
     3721 gttctgccac actgacaata atttcatcgc ccggttgcag acgcggacgg gcatagcatt
     3781 gcgccaccat gttgatggat tcagtggtgc cgcgcgtcca gacgatattt ttgtcatccg
     3841 atgcattcag taattgtgcc actttttccc gcgcagcttc gtaacgcgcg gtcaggcgtt
     3901 gggcttcggc aaactggctg cgatggacgt ttccggcgct caggctataa aactgtcggg
     3961 tggcttcaac cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg
     4021 catcctgtag cgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac
     4081 tcctcggctt gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa
     4141 ccatcagaat tatctggata tcctggcgaa gccgcgtcag taacatatgc tgaatagtgt
     4201 taggcgttaa ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag
     4261 aagatgatga aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc
     4321 gcctgttccg gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac
     4381 ggcaaaccac agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac
     4441 aaacagcaga tcaaccgtac tgacgtgaaa gagatggtcg aactggatca gtaattcgcg
     4501 atcgcaaggt aaaaaaaaag caccgcaatt aggcggtgct acattaatca ctatggacag
     4561 acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc taccatggtc tgaatcgcag
     4621 accaattgca aacacaacaa cacaacatca caaccgtaag ccaaaagttc accagaacac
     4681 gcattccgat aaaacttttc gttccggctc aggaagtgcc gccactatag gtatttgctg
     4741 gtagaagctc aacggacaat ttataatggc tcagattaaa aaaactaata ggttacacag
     4801 tgtaatctaa ttgttaaatt catttaacat caaagtttaa aagccatgtc taaacgatta
     4861 ccaccgctaa atgccttacg agtttttgat gccgcagcac gccatttaag tttcactcgc
     4921 gcagcagaag agctttttgt gacccaggcc gcagtaagtc atcaaatcaa gtctcttgag
     4981 gattttctgg ggctaaaact gttccgccgc cgtaatcgtt cactcctgct gaccgaggaa
     5041 ggtcaaagct atttcctcga tatcaaagag atattttcgc aattaaccga agcgacgcgt
     5101 aaactccagg cccgtagcgc taagggggcg ttgacggtca gtttactccc cagtttcgcg
     5161 attcattggt tggttccgcg actttccagc tttaattcag cttatccggg aattgacgtt
     5221 cgaatccagg cggttgatcg tcaggaagat aagctggcgg atgatgttga tgtggcgata
     5281 ttttatggtc ggggcaactg gccggggcta cgggtggaaa aactgtacgc cgaatattta
     5341 ttgccggtgt gctcgccgct actgctgact ggcgaaaaac ccttgaagat cccggaagat
     5401 ctggctaaac atacgttatt acatgatgcg tcacgccgtg actggcagac atatacccga
     5461 cagttggggt taaatcatat caacgttcag caagggccaa tttttagcca tagcgccatg
     5521 gtgctgcaag cggctatcca cgggcaggga gtggcgctgg caaataacgt gatggcgcaa
     5581 tctgaaatcg aggccggacg tcttgtttgc ccgtttaatg atgttctggt cagtaaaaac
     5641 gctttttatc tggtttgtca tgacagccag gcagaactgg gtaaaatagc cgcctttcgc
     5701 caatggatcc tggcgaaagc cgctgctgaa caagaaaaat tccgctttcg ttatgaacaa
     5761 taatttacgt agggtacgac catgaccagc cgttttatgc tgattttcgc cgccattagc
     5821 ggcttcattt ttgtagctct gggcgctttt ggcgcgcatg tgttaagtaa aaccatgggg
     5881 gccgttgaga tgggctggat ccagaccggc ctcgaatacc aggcgtttca tacgctggcg
     5941 atcttaggtc tggcggtggc aatgcagcgt cgcatcagta tctggtttta ctggagtagc
     6001 gttttcctcg cgttaggcac ggtgctgttc agcggcagcc tttattgcct ggcgctgtcc
     6061 catctgcgtt tgtgggcgtt tgtcacaccg gttggcggcg tgagcttcct tgcgggctgg
     6121 gcgttaatgt tagttggtgc tatccgttta aagcgcaagg gcgtaagtca tgaataaggt
     6181 tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc gcagaaatta ccgataaagc
     6241 cggccagcgg gaaattttcg gttttgcccg cgtgaaagag aatgcgggtt atgtcattta
     6301 tgaatgttat caacctgatg atggcgataa gttaatccgt gagctgccgt tcagttcatt
     6361 aatttttgcc cgccagtggt ttgtagtggg ggaactcctg cagcatttgc cgccagaaga
     6421 tcgtattacc cccattgtcg gcatgttaca gggcgtagta gagaagggcg gtgaactgcg
     6481 tgttgaagtt gccgatacca acgaaagcaa agagttgctg aaattctgcc gtaaattcac
     6541 cgtgccgcta cgcgctgcct tgcgtgatgc gggggtgctg gcgaactatg aaacgccgaa
     6601 gcgtccggtt gtgcatgtat tctttattgc accaggctgt tgctatactg gttactcata
     6661 cagcagcaat aattcgccgt tctatatggg cattccgcgc ctgaaatttc cggcagatgc
     6721 gccgagtcgt tccacgctca aactggaaga ggcatttcat gtgtttattc ccgcggatga
     6781 gtgggatgaa cgcctggcga acgggatgtg ggcggtggat ttgggcgctt gcccaggcgg
     6841 ctggacctac caactggtga agcgcaacat gtgggtttat tccgtcgaca acggcccgat
     6901 ggcgcaaagt ctgatggata ccgggcaggt aacgtggctg cgggaagacg gtttcaaatt
     6961 ccgtccgaca cgcagcaata tctcctggat ggtatgcgat atggttgaaa aaccggcgaa
     7021 agttgcggca ttaatggcgc agtggctggt taatggctgg tgccgtgaaa ctatcttcaa
     7081 tctcaaactg ccgatgaaaa aacgctacga agaagtgtca cacaatctgg cgtatattca
     7141 ggcacagctt gatgaacatg gcataaatgc tcagattcag gcgcggcagt tgtatcacga
     7201 tcgcgaagaa gtgacggtgc atgtccgccg tatctgggct gcggtgggtg gtcgtcgcga
     7261 cgagcgataa caggcaaggc cggggcgtcg tctccggcct gctacccttt ccgtcaggct
     7321 gttaccaaag aagttgtaac ctggcgctca tcagaaataa tatgcgttac ctcatccaga
     7381 tgcccgatgc gggcttcccc tgaacgatta aatttacttt tatcaatcaa taacaacgat
     7441 tgcgcggcac gttttaataa cattgatttg taatcagcgt tgatggcatt ggagtcccac
     7501 agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa acagatcgat ttccagcgat
     7561 ttaagttggg aaatcagcga gggattgacg taacagccat atttgcgctc aagtgtgccg
     7621 ccggaactga tcagttgaat gcgttcgcgt ttaccgagtt catggcaaat cggatggcta
     7681 ttggtgaaga cctggatgtt gatgtcaggc aactggcgtg ccagatacca gcaggttgaa
     7741 ctggcgtcta aggctatcac catcccttct tcaatccacg cgagcgcctc gcgcgcgata
     7801 tctgctttat gcgcataatg gcttttcagc cggatgtgaa agggatcgcc actgtcttga
     7861 ttttgacggt ggatatattt agcgcgccca tgattgcgca gaattttacc ctgcgtctgt
     7921 aattcattga gatcgcgacg aatggtttct ttactgacct ttagctgttc agagagagct
     7981 tccgtggtca ggctggtatg gttcagcagc aggtcgacta tcgcttgctg gcgtgccgct
     8041 ttcatatttt tcaccccatc attcgataac cgcccgcgtc atgcgggcgt accggtatga
     8101 gattacggtg ttaccccttt ttttaaaaga atattcccgt acttcgctcg ttcgcctgtg
     8161 ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg caaaacgatt gatgcggatg
     8221 atgtcaggac agggggcttg tagtgaaagc gcattacgat aacgtcgttc tacttcagga
     8281 tcgagagtgt caccttctac cgccgccatc atcaccagcg gtggtgcata actgtccagc
     8341 tcaaataacg ggataatcgc ctggagcaag tcgctcacca acaggccatc agcgcggatc
     8401 acctgcggtc ccatcgaatg ggcgggaaag tgagcatcgg aaaaaataat ttcatctcca
     8461 tgccccatct ctgccagcac tttcaatagt tcgggagaaa ttaacggcga aattgttttc
     8521 agcatttcac acctcctcta taaattcagg ttcagtttgc gggtagaaat aacggtactg
     8581 ataatgaatc tgtgcacggg cttcttccgg gctgttaaat tcccctacgc cataccaacc
     8641 aaacagcgca gctcctgcga cggtcgtttc ggcatcgtcg agaactttta ccggaatatc
     8701 aagcatattg gctttaatct gattccacaa tgtgttgcga cttcctccgc cgactaacaa
     8761 taattcagag gctttaaagt gcccgatttt ttccagcatc tgtagattgc gctgtaattg
     8821 cgtggttaac ccttccagcg ccgcgcgata gaaatgcccc cgcgtggtat taagcgttac
     8881 gccttgccag ccagcgttct ggcacgacaa taaatcacac tgcattttca ctccatctgc
     8941 gccaggcgcg atcaggcgag cttcttcaat caatatttgc cagggagttt cagccgtcca
     9001 gaacagtttt ctcacccatt ccagcacgcc ggatgccagc cattgcatac ctgggttata
     9061 caaccctgcc tggctatcca gttcgcaggt agaaccggcg tactgactta acagcgaagt
     9121 atcaacctgg gcgctgcgga ccattaaaat ttcccatgta ccggaagaga gcacgggttc
     9181 attttgttcg gcaccagcgc caaaaagggc gaactgggta tcgtgacctg cggaaatcac
     9241 cggtatgcca acgggtaagc cgagcattgc tgcggcgctg ttctgtagcg taccaatctg
     9301 ttcacccgct tccaccagac gagggaagag tcggcgtgga ataccggtgg cttgtaaaat
     9361 ttgcggactg aaatcgcgtt gctggatatc cagcatctgg ctggttccgg ccatcgtaat
     9421 atcagtagtg aattcgccgg ttaaacggtg gttaatcagc gacgaaataa agagccaggc
     9481 gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac cacaccaact tatataacgt
     9541 attgaaacta aaggctccga ctccagaaat agcctgcaac tgctgtgcgg agatcaaccg
     9601 ttcaatattg tccattaccg cggctgttcg cggacatttc cagctaatga tcggatagag
     9661 cagattgcct tgcttatcta ccagagcgcc atccacacca aaggtggtga cggcgatacc
     9721 gcggatgtgg caatcagtca gttcactatt gatttgccga cagcaatcag caaagcgttg
     9781 caaaatggcg tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc
     9841 attaggcgtt gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac
     9901 attggtcgcg ccacagtcga gtaccaggat aacttcttgt ttcataatcg ctcctgcaat
     9961 atagccggat aacattgctt atccggctaa ccactcttgc ttgttcaggc ccggtagccg
    10021 gagcgaccgg gcatcacatc agggagtcat gtattaacgc ttgtacaacg ggccgtagtt
    10081 ctggcaagcg cggtaatcct ggccttcaat atccatgccg tgcgcggccc aggcagaagg
    10141 acgatacact ttggtctctt caacgttgtg catacatacc gggatacgca gcatggaggc
    10201 aagagtgata aagtctgcgc caacgtggcc gatggtcaga accccgtggt tagcgcccca
    10261 gttcgccatt accgagtaca catccgtaaa cggcccttta ccggtgaggc gcggtgcaaa
    10321 ccaggtggtt ggccaggttg agttggtgcg tttgttgagg atgtcatgca catccttcgg
    10381 caattccacg ctccagcctt ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt
    10441 gacacgagtc atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc
    10501 gccacggaag tattcgtgga tcgccgggca ccattcggta gcggcgaggc aagcgtcagc
    10561 ctcttgctga gagatttccc agtgcggctt catcgtcggt ttaccttcgc tgtcgcgttg
    10621 tttacaggag ccgtccagcg cagcagaacc ggagttgatc aaatggatga tgccgtgttc
    10681 tgccagcccg tccagtttat gccccgttac acgctcaatt gcttctggtg accagtaggt
    10741 acggacatcg gcaaatacct gagcggtgcc ggtgagctgg tggcccatta acattgccac
    10801 gccgttaaga ctgtcgtttt cggtcgccac gacaaacggt ttgcgcacgc cattccagtc
    10861 aaatgaactg ttgaggatcg cttcagcggt atcgccattg ggatattgat cggtccagtg
    10921 acgttgcccc tggaagcctg cagcgatggc gttgtagcca agtgattctt ccacgcgacc
    10981 aatatcggcc agtttgctgt tgccttgcat catgtcgcgg atacacatcg ccatcagtaa
    11041 actttcgcgc agaactgcgc ggctttgctc ggcattgcgt tgatactgtt tgttattttc
    11101 atcttcgcca tagcggaagt ttttatcagc ccaggccagc gccatttcca gttcggcttc
    11161 gtcgtaaatc ttctgatcga tacggcgacg cagttcggtc atatccaccg cctggacttt
    11221 cattcccagc caggattcaa agaagttgtg atcaacgatg gaaccggcga tacccatcga
    11281 aacgccgccc agcgacagat agcttttacc tttcatgctg gcgactgcta aaccggcgcg
    11341 ggcaaagcgc agcagttttt cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg
    11401 aacgtcatga ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag ctgccagagc
    11461 cgctgccagg taaacagcgc cgggacgttc agtgccgtta aagccccaaa tggctttcgg
    11521 gcgggttgga tccatatcaa tggtttcact gccatagcac cagcaagggg ttaccgtaat
    11581 ggtgaggcct acattctgac tgctgaattt ttcttcgcaa gcagcggctt cagccatacc
    11641 cgcgatacag gtatcggaaa tgacacattc gacggcagct ccacaggcat ggcgcagttt
    11701 ctcggtcagc agtgcagccg tggctttcgc catattcatt gtttgttctt caagcgactc
    11761 acgaacaccc atgcgacgac cgtcaataac cgggcggata ccaattttcg gtaagctgat
    11821 ttttttcatt actgattcct cactttattc ggaaaatatg ttcagttagt tgccgtttga
    11881 gaacggaaac gggcaaagat aaagatgact gcgaagcaga gtgccgggat cagttcggca
    11941 gtggggatgt tgcccgctgc gtcactgaca aaccccatga ccggagtgac aataccgccg
    12001 ccaataatgg tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc gagattctta
    12061 atgcccagcg agaagattgt tgggtactga atcgacataa aggcactgca taaagtcagg
    12121 gctattaaac ccacatgacc gccagcgaaa gctgagatca ggcacagtgc catagcgatt
    12181 aatgcgtagg ctgccaggac tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg
    12241 aaacgaccaa taaagaagca caccatggtt ccggttaaat agttagcggc aaagcctgct
    12301 gtcatacctg gaatttcttc tacagcgtag cgaattaaat agctccagca ggccgtttgt
    12361 gcgccaacgt agcagaattg cgctaatact gcccagcgcc agtggcgaat acgcgccagg
    12421 cgagaaagcg atgcggagaa cgatccttgt ttggcgtcac tgtgattatc actctgcaat
    12481 gccgggaatt tcgtcagcat gatcagcagg gcgaccagta acacgatagc cacgatgatc
    12541 atataaggtg tctgtaccga taataccagg ctgtgtttat acgcactcaa ttgctctgga
    12601 gacattttat cgagaacgtc ttgcgattga tgtggcacgt tagacaaaat aagactttgc
    12661 ccaaagacaa ccgcgataat tgcgccaaat gagttaaatg tttgcgcaag atttaagcgg
    12721 aagtgaccac tgctttccgg ccctaatacc gtaacaaaag ggtttgcggc agtttccaga
    12781 caacctaatc cggctgcaat aataaatagg ccaactaaaa acaaggtgta gttcattatt
    12841 tctgcggcgg gccagaataa tgcagcgccg aaggcatata aaaacaatcc ggtaataatc
    12901 cctgctttat aactgagttt tttcatcaat atcccggcag ggattgggat aatgaaataa
    12961 ccaaagtaaa aggccgattg gatcaggcca gcctggaaat ttgtcagcgt aaaagcctgc
    13021 tggaattgag gcaataaaat gtcgttaagg ttattggcta ccgcccaaag aaaaaacagt
    13081 gagcacagca gcgcgaatgg aataatgtaa cttctgcttt gccctgcatc tttatctacc
    13141 gcacggtaac tctgcgtttg tattgatgtg tttcccatag catcctctta ggttcagaag
    13201 cttaagcacg taatcggtta tgttttcaaa agtgcaaaac cagcgagttc gggcttcata
    13261 tcagtgaata tttgcctgtc tgaaatgaac tcatggattc attttcaaat aaaaaacggt
    13321 ctgtaatatg acggcggtca cacttattag ctggaaggtg actttatgtg actaccatca
    13381 ctttaataat cgaaatttca tgcccgtttc agaaaataaa tcataaaaaa cacaaaccag
    13441 atgaccgtat taattgtttg tgccgaaaac gggcattcct ggcagggagg agtagaaaaa
    13501 tgaccgtttt gataaatatt cttaagagcg gtcattttta atgtggttat aaaaagagaa
    13561 attgcagtta tttactgtga ttagttgaac caggtcacaa aatataagct tgtgaatttc
    13621 tttctggtta atcaggaaga atatggtgag taattaaacg gctaattcaa tagtgtgaaa
    13681 ggaacaacat tattgccctg ttttgaatca gagagaggta gctaatggaa cgaaataaac
    13741 ttgctcgtca gattattgac acttgcctgg aaatgactcg cctggggctg aaccagggga
    13801 cagcgggtaa cgtcagtgta cgttatcagg atgggatgct gattacgcct acaggtattc
    13861 catatgaaaa actgacggag tcgcatattg tctttattga tggcaacggc aaacatgagg
    13921 aaggaaagct cccctcaagc gagtggcgtt tccatatggc ggcctatcaa agcagaccgg
    13981 atgccaacgc ggttgttcac aatcatgccg ttcattgtac ggcagtttcc attcttaacc
    14041 gaccgatccc cgcgattcac tacatgattg cggcggctgg cggtaattct attccttgcg
    14101 cgccttatgc gacctttgga acacgcgaac tttctgaaca tgttgcgctg gctctcaaaa
    14161 atcgtaaggc aactttgtta caacatcatg ggcttatcgc ttgtgaggcg aatctggaga
    14221 aagcgttatg gctggcgcat gaagttgaag tgctggcaca actttacctg acgaccctgg
    14281 cgattacgga cccggtgcca gtgctgagcg atgaagagat tgccgtagtg ctggagaaat
    14341 tcaaaaccta cgggttacgg attgaagagt aatttcgtaa agcaacaagg agaaggatga
    14401 tggctaacag aatgattctg aacgaaacgg catggtttgg tcgaggtgct gttggagctt
    14461 taaccgatga agtgaaacgc cgtggttatc agaaggcgct gatcgtcacc gataaaacgc
    14521 tggtgcaatg cggcgtggtg gcgaaagtga tcgataagat ggatgcggca ggactggcat
    14581 gggcgattta cgacggcgta gtacctaacc caacaattac tgtcgtcaaa gaagggctcg
    14641 atgtattcca gaatagcggc gcggattacc tgatcgctat tggtggtggt tctccacagg
    14701 atacgtgtaa agcgattggc attatcagca acaacccgga gtttgccgat gtgcgtagcc
    14761 tggaagggct ttccccgacc aataaaccca gtgtaccgat tctggcaatc cccaccacag
    14821 caggcactgc ggcagaagtg accattaact acgtgatcac tgacgaagag aaacggcgca
    14881 agtttgtttg cgttgatccg catgatatcc cgcaggtggc gtttattgac gctgacatga
    14941 tggatggtat gcctccagcg ctgaaagccg cgacgggtgt cgatgcgctc actcatgcta
    15001 ttgaggggta tattacccgt ggcgcgtggg cgctaaccga tgcgctgcac attaaagcga
    15061 ttgaaatcat tgctggggcg ctgcgaggaa cggttgctgg tgataaggat gccggagaag
    15121 aaatggcgct cgggcagtat gttgcgggta tgggattctc gaatgttggg ttagggttgg
    15181 tgcatggtat ggcgcatcca ctgggcgcgt tttacaacac gccacacggt gttgcaaatg
    15241 ccatcctgtt accgcatgtc atgcgctata acgctgactt taccggtgag aagtaccgcg
    15301 atatcgcgcg cgttatgggc gtgaaagtgg aaggtatgag cctggaagag gcgcgtaatg
    15361 ccgctgttga agcggtgttt gctctcaacc gtgatgtcgg tattccgcca catttacgtg
    15421 atgttggtgt acgcaaggaa gacattccgg cactggcgca ggcggcactg gatgatgttt
    15481 gtaccggtgg caacccgcgt gaagcaacgc ttgaggatat tgtagagctt taccataccg
    15541 cctggtaaat acgctgatgt gataatgccg gatgcgacgc tagccgcgtc ttatccggca
    15601 tataagtgca tgaaattcaa tgtgttgata atcgtttgca tccggcacgc cgttacctta
    15661 ccaaccgcaa ttgctgtaaa ttgccgtcga tatgcaaatc ggtttgtaag cgggcaatat
    15721 cgcggcacag aaacgccatc tctttatggg tttctaattt tttgcgccac ttttcggcaa
    15781 ccgcatccag attctcatat atcccttcca gactctgaaa ctcgaccagt agctgtgtgg
    15841 cgctttttgg tccgatccct gcaacacccg gtacctttga actgctgatc cccgcaagcc
    15901 cccagtaatc gggcaactgc tgcggttgaa cgccaaattc tttatcgata aatggcgcat
    15961 ccagccaacg tttctggaag taatcacgaa tacgtaatgt cggtgaaagt aactgacagt
    16021 agcctttatc tgtcgaaaca atcgttgcct gatgcccggc ctgtgtcact ttgaccgcca
    16081 gcgtggcggc taagtcatcg gcttcgttgc cgctggcgga ccagcacggg acgccgcgtt
    16141 gctcaaaggc ggcgcgtaat gcaggcatct cgttgtgcaa ctcttccggc attggcggtc
    16201 gacccgcttt gtaatctggt aaacgctgat gacgccagcc gctactgcgg ttttcatcat
    16261 caaaaacggc gaccgcgtgg gttggctggc tgtgcataat gagctgatcg agcgcgtgct
    16321 ggcaggtttc gacacagggc gaaccctgaa cggcatgaat gcgacgaata agattcagtg
    16381 catcgacaat aagcaaatga acagccacga taaccccctt aaagatcagg cgataagggt
    16441 aacattcgat gccagaacag gctatggctg gatgaggaat cgggaagagg cctcgcaaaa
    16501 agaggcctct ggagagcgat taatcgcagg caacgatttt cattgccagg ccgccgcgag
    16561 acgtttcacg gtacttggcg ttcatatctt tacctgtttc gtacatggtt tcgataactt
    16621 tgtcgaggca gacgcgtggc tcgctggtac ggcgcagcgc catacgtgca gcgtttactg
    16681 ctttcactgc cgcaatggcg ttacgctcga tgcacggcac ctgtacctgc ccggcgaccg
    16741 ggtcacacgt cagaccgagg ttgtgctcca tggcgatttc cgccgcgatg catacctgcg
    16801 ctgggcttgc gcctaacagt tctgccagac cagctgccgc cattgagcac gccacgccga
    16861 cttcaccctg gcagccgact tcggcaccag aaatcgaggc gttcatctta taaagagaac
    16921 caatggcgct ggctaccagc aggtaacgag ccagtgagtt agcgttcact tcgcggataa
    16981 acttgtcgta gtacgccagc acggccggaa taatcccgca cgcaccgtta gtcggcgcag
    17041 tcaccacgcg accgccagca gcattctctt cgttcactgc cagtgcaaac atgttgatcc
    17101 agtcaacaac cgccatcggg tcagtggtgg ttttatcctg gctgaccagc atccggcgta
    17161 gtgccgcagc acggcgtgga acgcgcagtt tgccaggtaa cacgccttcg gtagaaatac
    17221 cgcgctcaat accgccacgc atgacttccc agacgttcgc caggtgctgt tccagctctt
    17281 ctttgctgtg cagcgccagc tcgtttttca tcatcaggcc agagagtgac agcccggttt
    17341 cctgacaatg tttttgcaga tcggctgctg aactgtacgg ataaggaact tcaaccggtg
    17401 cgctatcctg ctgaccaaaa tgttcttcat caacaataaa gccaccgccg atagagtaat
    17461 aagtctggct gtaaacgact ttatcgcccg ccagcgcggt aatgcgcata ccgttttcat
    17521 gcagagaaag gttgtcggca tggaagttca tgcactgatc aaccgggaac tccacttcat
    17581 gctgaccgtt tgccagcatc aggcgaccat gagtattcac atcctgaata aaaccaggga
    17641 tggaatcgat atccacggta tccggcaggt tacccgccag acccataatt atggcgatat
    17701 ctgtgtggtg gcctttaccg gtcagagaga gcgagccgta cacgtcaacc accacgcggg
    17761 tcacgtcttt aagcaggtta cgggcaatca gatcgtcggt aaattgttta cccgctttca
    17821 ttggtccaac ggtatgagaa ctggaagggc caatgccgat tttgaaaata tcgaatacgc
    17881 taatcatagg aaatacatcg cgttaaaacg gaggaagcgc cgcccgaaag cggcgcgaaa
    17941 ggacttagct gaacagagag tagaagattg cggagattgc aatcagaccc atcacgacaa
    18001 cgaatacgtt gctgatgtga ccgctgtatt tacgcattgc cggtactttc tgaattgcgt
    18061 acatcggcat caggaacagg atcatcgcga tgattgggcc gcccagggtt tcaatcatac
    18121 ccaggatgct cgggttcagg gtggcaacaa tccaggtcgt taccagcatg aacagcgcag
    18181 tgatacggtt cagcttgttg atttcgatag acttaccttt accacgcaga gatttaatca
    18241 ccataccgtt gaagccttca cgtgcgccca ggtagtggcc gaggaaggat ttggtgatag
    18301 cgataatcgc gataatcgga gccatccacg cgatgatcgg cgcgttaaag tggttagcca
    18361 ggtaagacag aatcgagatg ttctgctctt tagccgcagc caggtctgcc ggagtcaggc
    18421 tcagtacgca gctgaagacg aagaacatta cggtcagcac catcatgatg tgtgcgaatg
    18481 ccaggatctt cgagcatttc tgttctgcca tatcgccgta ctcttcacgc ttcgcaacgg
    18541 cgaaagaaga gatgatcgga gagtggttga acgagaacac cattaccgga attgccagcc
    18601 acagtgtcat ccacagaccg tttccggttg cagatgcagt gtccagagac agcgtttcca
    18661 gtgctgcgcc gttccactgc gggatcaggt acagagccag cagcatcagt acgccaacaa
    18721 acgggaatac cagaatactc atcgctttaa cgatcatctg ctcaccgaag cgaacgatgg
    18781 tcatcatacc cacgatcagg atcagcgaca gaatcgcacg cggcggtggc gtcataccca
    18841 gctggtgaga catgaagctt tcaactgtat tggtgattgc cacgctataa accagcagga
    18901 tcgggtagat agcgaagaag tagagcagag taatcagttt acctgcgcca ataccaaagt
    18961 gttcttctac aacctcggtg atgtcttcgc ccgggttttt accagacagt acgaagcgag
    19021 tcaggccgcg gtgagcaaaa aacgtcatcg ggaacgcaag gatagccatg atgatcagcg
    19081 ggatcatacc gccaacacca gcgttgattg gcaggaacag cacgcccgcg ccgattgccg
    19141 tgccgtaaag gcccagcatc cacatggtgt ctgtcttgcg ccaggcacta cgagagtctt
    19201 tcgacgcaat cgtgctggtt tgagtcgttt ccatctattt ctcctggagg aatgcaaata
    19261 attcagcttt tagccagttc agcccgggaa ggtgcctgac cgtaaaaatg aaatctgtct
    19321 atcggcgatg gcttaatcat tttccaccgt aataatttga gggcggaaag atacacacaa
    19381 gttatgtatc tatcagtgat cttgatccca aatacaataa tctacttgga agatagataa
    19441 tcacaacgct ttactctgta aatcaaaagt aaaagagaat ttaagggcaa tatttaccca
    19501 ttaaacgaca tctgacttct tacggggcgc gcaatatatg taggttgaac tgaaaaaatc
    19561 aatactttta gttaatcatg gcgataattt ctttttcatg gcgataaaat gtggtttatg
    19621 aaaataggga aacgtttgcg tttcaacata aattgtatga catattaact gtggtgatat
    19681 ttgtcgcgtg agggtggtaa gaaacattca tataacaaat gaaatgagtt ggtgagtaag
    19741 tgaccaaaaa cgcgaaaacc cggcaacaaa cgtcaccggg ggaggggaga ttatgtgcag
    19801 atttcgtagc aagggatgta ggctgagcct ggcaacttca tacgatgctg ggcaacaaaa
    19861 ccctgtagca ggtcgtccat acgacgcata atctctttat cgccgttgat tttgtaagga
    19921 ccaaactctt caatggcgcg aataccgact tcttttacat tacccgccac aataccggag
    19981 aacgcacggc gcaggtcggc agccagcact tcaacgggtt gatccgggta aagcttcaga
    20041 ttagccatat tctcgtgaga cggctcaaac ggcatttgca aatctggcgc aatgcgcatt
    20101 gaccagttaa agctgtaagc gtcaccagtg tcgcggcgat tttctttcac cagcggcatc
    20161 gattttttca tctgacgagc gacttcagcg gcgtcatcaa tgatgatgcg gtaatggcgg
    20221 cgcgcgtttt cacccagcgt atgcacgaca aactcgtcca gtacgcggaa gtagtcggcg
    20281 ctctctttcg ggccggtgag gatcaatggt aaaacctgat ctttgttggc cgggttcatt
    20341 aaaattccca gcaaatacag caactcttct gctgtaccca caccgccagg gaagataatg
    20401 ataccgtgag cgatacggac aaacgcttcc agacgttttt cgatatctgg catgatgatc
    20461 aattcgttga ccagcgggtt aggcggttca gcggcgataa tcgacggctc tgtcatacca
    20521 ataaagcgac tgtctttgta acgctgctgc gcgtgtccga ccgcagcacc tttcatcggc
    20581 gcttccatcg ctcccggacc acagccggtg cagatattca gctcacgcag gcccagctga
    20641 ttcccgacgc gacgggcata caaatactcg ttttcgttaa ttgagtgacc gccccagcag
    20701 accaccatat ttggcgcttc accgacatgc agcgcgcgag cgttacgcaa gatggaaaag
    20761 accaggttag tgatgtggac ggagttgtcg agattcaggt tgggaaaacg aacggtgtta
    20821 tgaatttgcc cataaacgaa gagaatgtca cgcagaaccg cgaacaagtt ggcctgcaaa
    20881 gcgcgaataa ttcgcccatc gacaaaagcc tcttccgggg gatttatcag ttccagcttt
    20941 acgccgcgtt cacggcgcag gacgttaata tcgaaatttt caaaacgaga cagcaattct
    21001 ttgctgttat cggtcaaact accggagttc agtacggcaa gtgaacagtt gcgaaacagt
    21061 tgatagaggt cgctgctggc ggtgcgttta agcatatcca cttccagctg cgacaacata
    21121 tccatggagc caagcgggct aatatgtgta atcaagaaaa actccttatg ggacgaatat
    21181 cgtcattccc tttgattaca atagccctgc ctgctgacct ttcacaacct tctgcgtgaa
    21241 tccagcacgc aaaattgaga aaaaatttat tgccgaacca gtcttgtggt cgatgggaca
    21301 aaatcgctat tactgcgcca cgggttaatg tccagaccgc cacgacgggt ataacgtgcg
    21361 taaacgctca atttctctgg ctggcagaag cgtaacaggt cattaaagat gcgttccacg
    21421 cactgttcgt ggaactcgtt gtgatgacgg aaagaaacca ggtaacgcag cagtttttct
    21481 ctgtcaattt ggcgtccacg atactgaatt tggatcgatc cccaatctgg ttgatgggtg
    21541 atcaggcagt ttgatttcag caggtggctg acaagcgtct cttccactac tttttcaccg
    21601 ctggtggcat tctccagata gtcagtagtg aattcatagt tatcgatagt gatatcctgg
    21661 tcatcaatgc aggtgccatt gaaatgacct atcggctggc cttccagttc atcaagacga
    21721 tataacgcca cgctaacctt accctgggcg caagtgctta agtcgcgctc cagcgtctgg
    21781 cgcacctcat cccagttatt aaaacgcgtc tggttaaaac tgttgagata gagcttaaaa
    21841 ctcttcgact caatcagatt tacgctggtg taatcaagtt caacatgacc gacagcgacc
    21901 tgcggcaaac ctttcgcatt cagccaggaa agttcataca gcgtccagat atccgtaccg
    21961 tgaaaaggca ggttatccgc tttcagaccc agcgggtcgc gattcaggct gcgtggaacg
    22021 ccttgcagta ggctggcgtc ataggtatcc cggtaatcgg ttgattttcc aagtgtcagg
    22081 cccgcaagtg cctgatggtt tgcataagaa gacatgtttc accgtcataa accagataaa
    22141 ctaaacgaca agtgtatctg gtttaagtga agagagaaat tagtggacga tttgaccgca
    22201 caagccctga aagattttac tgcacgctac tgcgatgcgt ggcatgaaga gcataaaagc
    22261 tggccgttaa gtgaggaact gtacggcgtt ccttcaccgt gcattatttc aactaccgaa
    22321 gatgccatat actggcaacc ccagccgttt accggggaac aaaatgtaaa cgcggttgaa
    22381 cgcgcttttg atattgtgat acaacccacg attcatacct tttatacgac tcagtttgcc
    22441 ggggatatgc acgcgcagtt tggcgatatc aaactgacat tgctgcaaac ctggagtgaa
    22501 gacgacttcc gtcgggtgca ggaaaatctg attggtcatc tggtgactca gaaaaggcta
    22561 aaactgccgc ccacgctatt tatcgcgacg ctggaagaag agttagaggt catctcggtg
    22621 tgtaatctct ccggtgaagt gtgcaaagag acgctgggaa cacgtaaacg gacgcattta
    22681 gcctcaaatc ttgcggaatt ccttaatcaa cttaagcctc ttctgtaatc caaatacccc
    22741 atctggttgt gagagatctc ttacagactc tgtaggagat cgccaggaaa taagcgaata
    22801 cttaaaaaga taagaatcgt tattctcatt taaaaacaac atgttgcttg ttaattctta
    22861 actttcatat gaaattttcc ttaaggcatt gtctgtaagc gccttgtaag acttcgagaa
    22921 aaagacgatt ctatcttcgt cgacagggag tcagacaacg aagtgaacat caggatgatg
    22981 acacttctga aggacacacc aggatggtgt ttcagggaaa ggcttctgga tgaagcgaag
    23041 aggatgacgc aggacgcgtt aaaggacacc tccaggatgg agaatgagaa ccggtcagga
    23101 tgattcggtg ggtcaggaag gccaggaaca cttcaggatg aagtatcaca tcggggtggt
    23161 gtgagcagga agcaatagtt caggatgaac gattggccgc aaggccagag gaaaagttgt
    23221 caaggatgag cagggagcaa caaaagtagc cggaatgctg cgaaacgaac cgggagcact
    23281 gtgaatacag tgctcccttt ttttattcct gctattcttc gcggcagttt ttcttattga
    23341 ggttgcttta tgaccactca tgaccgcgtt cgtctccagt tgcaggcgct tgaagcgtta
    23401 ctgcgtgaac atcagcactg gcgaaacgat gaacccctgc cgcatcaatt taacagtact
    23461 caaccgttct ttatggacac catggaacca ctggagtggt tgcagtgggt gctgatcccg
    23521 cgaatgcacg atttgttaga taacaaccag ccgttaccgg gcgcttttgc tgttgcgccc
    23581 tattatgaaa tggcgctggc gacggaccac ccgcaacgcg cgcttattct ggcagagctg
    23641 gaaaaactgg atgctctttt tgcggatgat gcgagctaat gctggaaata ctctatcagg
    23701 atgaatggct ggttgcggta aataaaccct ccggctggtt ggttcaccgc agctggctgg
    23761 atcgcgacga gaaagtagtg gtcatgcaaa ccgtgcgtga ccagataggc cagcatgttt
    23821 ttactgctca tcgtctggac cgaccaactt ctggtgtgtt gttgatggga ttatccagcg
    23881 aagccggacg gctgctggca caacagtttg aacagcacca aatccagaaa cgttaccatg
    23941 cgattgtgcg tggctggttg atggaagaag cggtgctgga ttatccactg gtggaagaac
    24001 tggacaaaat cgctgataaa tttgcccgcg aagataaagg cccgcagcca gcagtgacgc
    24061 attatcgcgg tctggcgacc gtagaaatgc cggtagcgac cggacgttac ccgaccacgc
    24121 gctacggcct ggtggaactg gagccgaaaa ctggacgcaa gcaccagctt cgtcgccatc
    24181 ttgcccattt gcggcacccg attattggcg acagcaaaca tggtgattta cgccagaatc
    24241 gcagcggtgc tgagcatttt ggccttcagc ggttaatgct gcatgccagt cagttgtcat
    24301 tgacgcatcc ttttactggc gagccgctga ctattcacgc gggcctggac gacacctgga
    24361 tgcaggcgtt atcacaattt ggctggcgcg gtctgcttcc tgaaaatgaa agggttgagt
    24421 tcagtgcgcc atcgggtcag gatggagaga taagttccta atccagggag aaaacggtat
    24481 ggcggaaatt ggtatttttg tcggcaccat gtacgggaat tcactgttag tggccgaaga
    24541 agcggaagcg attctgaccg cgcagggcca caaagcaacg gtatttgaag atcctgaatt
    24601 aagcgactgg ctgccctatc aggataagta tgttctggtg gttacgtcca cgaccgggca
    24661 gggcgacctt cctgatagca ttgtgccact ctttcaggga atcaaagata gtctgggctt
    24721 ccagccgaat ctgcgttatg gcgtgattgc gctcggcgac agtagttatg tgaatttctg
    24781 caatggcggc aaacagttcg atgccttgtt gcaggaacag agcgctcagc gggttggcga
    24841 aatgctgttg attgacgcca gtgagaaccc ggaaccggaa acggaatcaa atccgtgggt
    24901 tgaacattgg ggcacgatgt tgtcctgata tgttcagcga gcggtaaatg ccgttttagc
    24961 ggtgctgaat cgaatctttt caggcaaatg ccagtaaaaa ctgcttcata gcgcggattt
    25021 ttactggcgt ttgcctggag tcaagcgatc catttcatac tcttctttat ttcttcgttt
    25081 taacccttcc tttcttgttc ttgttttcat ttccgtgaag tggattccac cgtccagggc
    25141 taatgccaaa atcgggcctc attgaacgca ttaatgttgt gttgttgcac ggtgagccgc
    25201 tatggcgcgc tttttatact gctattgcca gatataaaca cgcgccgtat tcggcgaacg
    25261 acctataaaa acggcaaaaa caccctacgt cacctctgat ttcctggcga tgtcgcagtc
    25321 cagagtgagc gtggctaacg cgaattttca ggagtgcaac aatgagttct ttaagtcagg
    25381 ctgcgagcag tgtggaaaaa cgcacgaatg ctcgttactg gatagtggtg atgttgttta
    25441 tcgtcacatc cttcaactac ggcgaccgcg ctacgctttc tattgccggt tcggaaatgg
    25501 ccaaagatat cggccttgat cccgtgggaa tgggctatgt gttctctgcc ttctcatggg
    25561 cgtatgttat cgggcagatc cctggtggct ggttgctgga tcgttttggt tcaaaacgcg
    25621 tctacttctg gtcgatcttt atctggtcga tgtttacctt gctgcaaggc ttcgtcgata
    25681 tctttagtgg attcggcatt atcgttgccc tgtttacact gcgcttcctg gtcgggcttg
    25741 ctgaagcgcc ctctttccct ggcaacagcc gcattgttgc ggcctggttt ccggcgcagg
    25801 aaaggggaac ggcggtgtcg atttttaact ccgcccaata cttcgcaacg gttatcttcg
    25861 cgccgattat gggctggttg actcatgaag tgggctggtc acacgtcttc ttctttatgg
    25921 gcggtctggg cattgtcatc agctttatct ggttgaaagt catccacgag ccaaatcaac
    25981 atccgggcgt aaatcagaaa gagctggagt acatcgccgc gggtggcgcg ctgatcaata
    26041 tggatcagca aaacaccaaa gttaaagtgc cgttcagcgt gaagtggggg cagatcaaac
    26101 agctgctcgg gtcacggatg atgatcggcg tttatatcgg tcagtactgt atcaacgccc
    26161 tgacttactt ttttattacc tggttcccgg tttatctggt gcaggcgcgc gggatgtcga
    26221 ttctgaaagc aggctttgtg gcttctgttc cggcggtttg cggttttatc ggcggtgtgt
    26281 tgggggggat tatttccgac tggctgatgc gtcgcacagg ttcactgaac attgcgcgta
    26341 aaacaccgat cgtgatgggc atgttgttgt cgatggtgat ggtgttctgc aactacgtca
    26401 acgttgagtg gatgatcatc ggctttatgg cgctggcctt cttcggtaag gggatcgggg
    26461 cgctgggttg ggctgtaatg gctgatactg cgccaaaaga gatcagcggt ctttccggtg
    26521 gcctgttcaa catgtttggt aacatttctg gcatcgtcac gccaatcgca attggttata
    26581 tcgttggcac gactggctcc tttaatgggg cgctgattta tgttggtgtt catgccttaa
    26641 tcgcggtact cagctacctg gtgctggtgg gcgatatcaa gcgtattgag ctgaaacctg
    26701 tcgtggggca ataagatgac gacacaatcc agtcctgtta ttactgatat gaaagtcatt
    26761 ccggtcgccg gacatgacag catgttgctt aatattggtg gtgcacataa cgcatatttc
    26821 acccgcaata ttgtggtact caccgataac gccgggcata ccggcattgg tgaagcgccg
    26881 ggcggagagg tgatttatca aacactggtc gaggctattc cgatggtgct gggccaggaa
    26941 gttgcgcgcc tgaataaagt ggttcagcag gtgcataaag gtaatcaggc agccgatttt
    27001 gataccttcg gcaaaggtgc ctggactttt gaattgcgcg ttaatgccgt ggcggcgctg
    27061 gaagccgctt tgcttgacct gttaggtcag gcgctgaatg ttccggtctg cgaactgtta
    27121 gggccaggca agcaacgtga tgctattacc gttctcggtt atctgtttta tatcggtgat
    27181 cggaccaaaa ccgatcttcc ttatctggaa aatacgccgg gcaaccatga gtggtatcag
    27241 ttgcggcatc agaaagcgat gaacagcgaa gccgttgtgc gtctggcgga agcctcacag
    27301 gatcgctatg gctttaaaga tttcaaactt aagggtggcg tgttacctgg cgagcaagaa
    27361 atcgacactg ttcgtgcatt gaagaaacgc ttccctgatg cgcggattac cgttgatccc
    27421 aatggtgcct ggctgcttga tgaagccatt tctctgtgca aagggctgaa tgatgttctt
    27481 acctatgcgg aagacccgtg cggcgctgag cagggtttct ccggtcgtga agtgatggcg
    27541 gagtttcgac gggcgaccgg cttgcccgtc gcgaccaaca tgatcgccac caactggcgc
    27601 gaaatgggtc atgcggtgat gctcaatgcg gtagatattc cacttgccga tccgcacttc
    27661 tggacgcttt ccggtgcagt ccgcgtggcg caactttgcg acgactgggg gctgacctgg
    27721 ggctgccatt ctaataacca ctttgatatc tctctggcga tgtttaccca tgtgggcgcg
    27781 gcggcaccgg gtaatcctac cgctatcgat acccactgga tttggcagga gggcgattgt
    27841 cgtctgacca aaaatccgct ggagattaaa aacggaaaaa ttgccgttcc tgatgcgccc
    27901 ggtctgggcg tggaactgga ctgggaacag gtacaaaagg cacatgaggc ctataaacgt
    27961 ctgcctggcg gtgcgcgtaa cgacgcaggc ccgatgcagt acctgatccc cggctggacc
    28021 tttgaccgta aacgtcccgt tttcggccgt cattgattct gaaaaaggac ataaatatga
    28081 gttctcaatt tacgacgcct gttgttactg aaatgcaggt catcccggtg gcgggtcatg
    28141 acagtatgct gatgaatctg agtggtgcac atgcaccgtt cttcacgcgt aatattgtga
    28201 ttatcaaaga taattccggt cacactggcg taggggaaat tcccggcggc gagaaaatcc
    28261 gtaaaacgct ggaagatgcg attccgctgg tggtgggtaa aacgctgggt gaatacaaaa
    28321 acgttctgac gctggtgcgt aatacttttg ccgatcgtga tgccggtggg cgcggtttgc
    28381 agacatttga tttgcgtacc actattcatg tagttaccgg gatagaagcg gcaatgctgg
    28441 atctgctggg gcagcatctg ggggtaaacg tggcatcgct gctgggcgat ggtcaacagc
    28501 gtagcgaagt cgaaatgctc ggttatctgt tcttcgtcgg taatcgcaaa gctacaccgc
    28561 tgccgtatca aagccagccg gatgactcat gcgactggta tcgcctgcgt catgaagaag
    28621 cgatgacgcc ggatgcggtg gtgcgcctgg cggaagcggc gtatgaaaaa tatggcttca
    28681 acgatttcaa actgaaaggc ggtgtactgg ccggggaaga agaagccgaa tctattgtgg
    28741 cactggcgca acgtttcccg caggcgcgta ttacgctcga tcctaacggt gcctggtcgc
    28801 tgaacgaagc gattaaaatc ggtacatacc tgaaaggttc gctggcttat gcagaagatc
    28861 cgtgtggtgc ggagcaaggt ttctccgggc gtgaagtgat ggcagagttc cgtcgcgcaa
    28921 ctggcctgcc gaccgcaacc aatatgatcg ccaccgactg gcggcagatg ggccatacgc
    28981 tctcgttgca atccgttgat atcccgcttg cggatccgca tttctggacg atgcaaggtt
    29041 cggtacgtgt ggcgcaaatg tgtcatgaat ttggcctgac ctggggttca cactccaaca
    29101 accacttcga tatttccctg gcgatgttta cccatgttgc cgctgccgca ccgggtaaaa
    29161 tcaccgcgat tgatacgcac tggatctggc aggaaggtaa tcagcgcctg accaaagaac
    29221 cgttagagat caaaggcggt ctggttcagg tgccagaaaa accggggctg ggtgtagaaa
    29281 tcgatatgga tcaagtgatg aaagcgcacg agctgtatca gaaacacggg cttggcgcgc
    29341 gtgacgatgc gatgggaatg cagtacctga tccccggctg gacgttcgat aacaagcgtc
    29401 cgtgcatggt gcgttaagac agaaaaccaa aaccggctct atctggagcc ggtttgagac
    29461 tgatgccaaa cgcaaaattg cctgttgcgc tacgtatcag ggctacatca ttttttaatt
    29521 cattgaattt aattggtttt gcaggccgga taaggcgtta acgccgcatc cggcataaac
    29581 acaggcactt gtcaccaatc tgaaaccagc gtcataaaaa tccggttgct actcgacaag
    29641 acgtccatta cccgagaatt ttgctggctt cgcgcgctac attatccatc tcgtccaaca
    29701 gttccagcag ctccggctct aaatcttctt ctttagtacc gctacgtagc tgttgttcga
    29761 tcagttggca gagattcttc atacgcggta caccgctata gccgcaactg ccatgcagtt
    29821 tatgaatcaa atcaaccagg ccttccgggt tttctccaac cagctgttcc tcaactttgt
    29881 tgcgaacttc aggcaggaaa tcgagtaaca tttgcagcat atcgcgcgct aaatcggttt
    29941 ttcctgctgc ctggcgtagt gccagttgcc agtcgagggt cgcattcggg ttgaccacaa
    30001 tttcgttgac ttcgggcgtc acgacgcgag aggaaatacc gctgccaggc ttgtagcgca
    30061 acagcaaatt atgcaatcgc tcttcttcga tcggtttggc cagataatca ctcatccccg
    30121 cgccaagcag tttctctttt tgcccggcca tcgcatgcgc cgttaccgcg ataaccggcg
    30181 tttgctgctg atgcgggagc tggtggatga gctcacatgc ccgaatgccg tccatgtccg
    30241 gcatttgaat atccattaag attaaatcga acggcatctg tttcgcccgt tcaaccgcct
    30301 gatgcccgct atcgcaaagt tccacatgtt gcaccatatc ttccagcaat gcgccgataa
    30361 gtttcaggtt agcggggttg tcatcaaccg ccatgactgt cattgccagc ttactttcat
    30421 cggttacagg caaaagcgtg ttttgtttgt gatgacaaaa ttccgtcagg gcaggcaaca
    30481 ggcgcgtagg tgttaatggt ttcagcagac acgcgccgat accatcttgc ttaagttttt
    30541 cagcattgac ttgtgcatgg caaggaagtg ccagcatcag gaaatcggtc atcgatactg
    30601 ctttcgctaa tcgctcatgt tgcattgtta gcggctcgcg gaaggtcacc gctatgccta
    30661 ataacatcat gtcgtaatgc gcgggaggca gcgcggagaa cgttgggcta taaaccactt
    30721 ccagcggcgt ttcacttaaa atatccagcg tgcattgtgc tgctgcggag tttggttcga
    30781 cataggccag gcgcttacca gcgaggcact gggtggatgg cccttcgata ataatgttcg
    30841 ggttcagatc gagattaatg tggaaccaga aagttgaacc acgattcggc aggctatgga
    30901 acgaaatatc gccgcccatt tcattaacca gtttttgtgt aatcaccagc cccagcccgg
    30961 tgccaccatg acggcgggaa atactggcat cagcctgtct gaaggcctgg aataagcgcg
    31021 attgatcgcg ttcaggaata ccaatgccgg tatcccgaat ctgcacttca atctgcactt
    31081 tggtattact cagcgcacgt ttttctacca gaatatcgat gttgccattc tcggtgaatt
    31141 taattgcatt ccctaccagg ttagtgatga tttgctgtaa tcgtagtggg tcgccgatca
    31201 cgttatcagg cacgtcgctt ttaatattga gcgtcagttc cagcccttta tcgtgagaag
    31261 aatgtgccag cagggtaacg acttcatcca gcgtgctgcg tagtgggaat ggaatacttt
    31321 ccagaatcag cttaccggct tccagtttcg agaagtcgag aacatcatta ataattgcca
    31381 gcaaattatt tgccgaacgt tcaatcgtat tcaggtgatc gcgctgcgtt ggtgttaatt
    31441 ctgttttcag cgtcaggcgg gtaaagccaa taacaccatt cagtggtgta cgcagctcgt
    31501 gtgacatatt tgccagaaac tcggatttaa tacgcgccgc ttcctgggcg cgctttttcg
    31561 ccagatccaa ctcaacgttc tgaatctcca tttgctccag cgtctcacgc agatcggacg
    31621 tcgcctggtc gatattgtgt tgcatctctt cgtgataagc cgccagcgac attgccatcg
    31681 agttgatacc gtttttcagc atatccagct cgccgagcat aaatccttcc acccggctgt
    31741 cgagttgccc gcgacggatg cggtcgacgg tattcaccat gttgcgaatc ggaccggtta
    31801 catcgcgcat taagcgccag ccaaaaataa gcgcaatacc gatacaaaac agcatcatca
    31861 cgctggaaat aaagatctct ttatattgct gcaagcgaac cgatttaaga tccagctcca
    31921 gcgcaatata tcccagcata ttttgactat ttttggcatc gctactgggc gattcgtcgg
    31981 gggagtaact ttcagaaata atcggcgtgc ggaggatcat tatatcgccg tcacgagtga
    32041 cagtgagctg gcgaggaaac ggcacgttgc tgccgagctg cattgatgag ggatcaagat
    32101 gaaaattgga ggtgacaaag agtcggttat tttcatcata aaccgaaatc gcgcgaacaa
    32161 tatcagaatg gcgacgatgc agtacgctta ttaactgacc gatagattcg cgattttgca
    32221 gactcatgcc atattcagta gaaactgcaa gcggctcaat aatgctggca ccggcatctt
    32281 ccagttgacg ctgcaagtcg ttatagcgat gcacgacgaa aaagatactc agcaataaac
    32341 caataaggac ggtcggtgcc aggatcagaa tcatcatgcg tgcgcgcagg ctgtagttgg
    32401 tcatggagtt ccgttatggg acaattaagg tcacactgtt aaattgagaa agtctcgcca
    32461 atggcgcaat tctactctgc aaaacgacgc acgacgacgc gtcagatcat aaccgtttca
    32521 gtcaacgacc tcgactcttt tggtcagggc gtggcgcgac ataacggcaa aacgttgttt
    32581 atccccggat tattgccgca ggaaaacgcg gaagttactg ttactgaaga taaaaaacag
    32641 tatgcccgcg ctaaagtcgt acgtcggtta agcgatagcc cggaacgcga aacgccacgc
    32701 tgtcctcatt ttggcgtatg cggcggctgt cagcaacaac acgccagtgt ggatttacag
    32761 cagcgaagca aaagtgcggc actcgcccga ttaatgaaac acgaagtctc tgaagtgatc
    32821 gccgatgttc cctggggtta tcgccgtcgc gcgcgtttaa gtctgaacta cttaccgaaa
    32881 acacagcaac ttcagatggg gtttcgcaaa gcgggctcca gtgacattgt cgacgtgaaa
    32941 caatgcccca ttttagtgcc ccaacttgaa gcattgctgc ccaaagtcag ggcatgcctg
    33001 ggcagcttac aagctatgcg ccatcttggt catgttgaac tggtacaggc aaccagcggc
    33061 acgctaatga ttttgcgcca taccgcaccg ctaagttcgg cagatcgcga aaaactggaa
    33121 tgcttttcgc attctgaagg cctggatctg tatctcgccc ccgatagtga gatactcgaa
    33181 accgtctctg gtgagatgcc ctggtatgac tcaaatgggt tgcgcttaac ttttagcccg
    33241 cgcgatttta ttcaggtcaa tgcgggtgtg aaccagaaaa tggtagcgcg tgcgttggaa
    33301 tggctggatg tggaacctga agattgcgta ctggatctgt tctgcggtat gggcaacttt
    33361 acactgccat tggcgacaca agctgccagt gtggtgggtg tagaaggtgt tccggcgctg
    33421 gtggaaaaag gccagcagaa tgcgcgtctt aatggcttac agaatgtgac gttttatcac
    33481 gaaaatcttg aagaagatgt cacaaagcag ccgtgggcga aaaacggctt cgataaagtg
    33541 ttgttggacc cggcgcgagc aggtgccgca ggtgttatgc agcaaattat aaaactggaa
    33601 cctattcgta tagtttatgt atcctgtaac cctgcaacgc tggctcggga tagcgaagcg
    33661 ttattaaaag caggatatac cattgcgcga ctggcgatgc tggatatgtt cccacacacg
    33721 ggacatctgg aatcgatggt acttttctcg cgcgttaaat agttacgatt tgccgatttc
    33781 ggcaggtctg gtccctaaag gagaggacga tggttgcggt aagaagtgca catatcaata
    33841 aggctggtga atttgatccg gaaaaatgga tcgcaagtct gggtattacc agccagaagt
    33901 cgtgtgagtg cttagccgaa acctgggcgt attgtctgca acagacgcag gggcatccgg
    33961 atgccagtct gttattgtgg cgtggtgttg agatggtgga gatcctctcg acattaagta
    34021 tggacattga cacgctgcgg gcggcgctgc tgttccctct ggccgatgcc aacgtagtca
    34081 gcgaagatgt gctgcgtgat agcgtcggta agtcggtcgt taaccttatt cacggcgtgc
    34141 gtgatatggc ggcgatccgc cagctgaaag cgacgcacac tgattctgtt tcctccgaac
    34201 aggtcgataa cgttcgccgg atgttattgg cgatggtcga tgattttcgc tgcgtagtca
    34261 tcaaactggc ggagcgtatt gctcatctgc gcgaagtaaa agatgcgccg gaagatgaac
    34321 gtgtactggc ggcaaaagag tgcaccaata tctacgcgcc gttggcgaac cgtcttggga
    34381 ttgggcaact gaaatgggag ctggaagatt actgcttccg ttatctccac ccgaccgaat
    34441 acaaacgcat cgcaaaactg ctgcatgaac gccgtctcga ccgcgaacac tatatcgaag
    34501 agtttgtcgg ccatctgcgc gctgagatga aagctgaagg cgttaaagct gaagtgtatg
    34561 gtcgaccgaa acacatctac agcatctggc gcaaaatgca gaaaaagaac ctcgccttcg
    34621 atgagctgtt tgatgtgcgt gcggtacgta ttgtcgccga gcgtttacag gattgttatg
    34681 ccgcactggg gatagtgcac actcactatc gccacctgcc ggatgagttt gacgattacg
    34741 tcgctaatcc gaaaccaaac ggttatcagt ctattcatac cgtggttctg gggccgggcg
    34801 gcaaaaccgt tgagatccaa atccgcacca aacagatgca tgaagatgca gagttgggtg
    34861 ttgctgcgca ctggaaatat aaagagggcg cggctgctgg cggcgcacgt tcgggacatg
    34921 aagaccggat tgcctggctg cgtaaactga ttgcgtggca ggaagagatg gctgattccg
    34981 gcgaaatgct cgacgaagta cgtagtcagg tctttgacga ccgagtgtac gtctttacgc
    35041 cgaaaggtga tgtcgttgat ttgcctgcgg gatcaacgcc gctggacttt gcttaccaca
    35101 tccacagtga tgtcggacac cgctgcatcg gggcaaaaat tggcgggcgc attgtgccgt
    35161 tcacctacca gctgcagatg ggcgaccaga ttgaaattat cacccagaaa cagccgaacc
    35221 ccagccgtga ctggttaaac ccaaacctcg gttacgtcac aaccagccgt gggcgttcga
    35281 aaattcacgc ctggttccgt aaacaggacc gtgacaaaaa cattctggct gggcggcaaa
    35341 tccttgacga cgagctggaa catctgggga tcagcctgaa agaagcagaa aaacatctgc
    35401 tgccgcgtta caacttcaat gatgtcgacg agttgctggc ggcgattggt ggcggggata
    35461 tccgtcttaa tcagatggtg aacttcctgc aatcgcaatt taataagccg agtgccgaag
    35521 agcaggacgc cgccgcgctg aaacagcttc agcaaaaaag ctacacgccg caaaaccgca
    35581 gtaaagataa cggtcgtgtg gtggttgaag gtgtcggtaa cctgatgcac cacatcgcgc
    35641 gctgctgcca gccgattcct ggagatgaga ttgtcggctt cattacccag gggcgcggta
    35701 tttcagtaca ccgcgccgat tgcgaacaat tggcggaact gcgctcccat gcgccagaac
    35761 gcattgttga cgcggtatgg ggcgagagct actccgccgg atattcgctg gtggtccgcg
    35821 tggtggctaa tgatcgtagt gggttgttac gtgatatcac gaccattctc gccaacgaga
    35881 aggtgaacgt acttggcgtt gccagccgta gcgacaccaa acagcaactg gcgaccatcg
    35941 acatgaccat tgagatttac aacctgcaag tgctgggtcg cgtgctgggt aaactcaacc
    36001 aggtaccgga tgttatcgac gcgcgtcggt tgcacgggag ttaatcttac atagctggat
    36061 atggcgtcag ccgtatccag cgatatcttt tactcgaagg tattacacct gacggacccg
    36121 caattttcag gataattcaa tgaatcaaat cgaccgtttg ctcaccgtca tgcagcgcct
    36181 gcgcgacccg gaaaacggct gcccgtggga taaagagcag acatttgcca ccattgcgcc
    36241 ttacaccctt gaagaaacct acgaggtgct ggacgccatc gcccgtgaag attttgacga
    36301 tctgcgcggt gaactgggcg atctgctgtt ccaggtggtg ttttacgcac aaatggctca
    36361 ggaagaaggg cgctttgact ttaatgatat ttgcgctgct attagcgata aattagagcg
    36421 tcgccatccg catgtttttg ctgacagttc tgccgaaaat agtagtgaag tgcttgcccg
    36481 ttgggagcaa atcaaaaccg aagagcgcgc gcagaaagcg cagcattcgg cgctggacga
    36541 tattcctcgt agtttaccgg ctttaatgcg tgcgcaaaaa atccagaaac gttgcgccaa
    36601 cgttggtttc gactggacga cgcttggtcc ggtagtcgat aaagtctacg aagagatcga
    36661 cgaggtgatg tacgaggcgc ggcaggctgt tgtcgaccaa gctaaactgg aggaggaaat
    36721 gggtgacctg ctgtttgcca cggtcaatct ggctcgtcat ttagggacaa aagcggaaat
    36781 cgcattgcaa aaagcgaacg aaaaattcga gcgtcgtttt cgcgaagtgg agcgtattgt
    36841 tgccgcgcgt ggactggaaa tgacaggtgt tgacctcgaa acaatggaag aagtctggca
    36901 acaggtaaaa cggcaggaaa ttgatctcta agggaattac gtggtcaagc gcgatttgtg
    36961 tcatttttta aatgacaagc gcttgatttg cgtcaaaaac atttacccca aaggggctat
    37021 tttctcactc ctgatttcaa tagtgcgctg gcgaagagga gggataatga aagtttgtgg
    37081 cacaggtcat gttcgggtat actgctttcc cgtcctggtt attccatcgt cttttcaacc
    37141 taacttctca ggttcagcat gacaacgaac tatatttttg tgaccggcgg ggtcgtatcc
    37201 tctctgggta aaggcattgc cgcagcctcc ctcgcagcca ttcttgaagc ccgtggcctc
    37261 aatgtgacca tcatgaaact ggatccgtac atcaacgtcg atccaggtac tatgagccca
    37321 atccaacacg gggaagtgtt cgttactgaa gacggcgctg aaaccgacct ggacctgggg
    37381 cactacgagc gtttcattcg taccaaaatg agccgccgca acaacttcac cacgggtcgt
    37441 atctactctg acgttctgcg taaagaacgc cgcggtgact atctcggcgc aaccgtacag
    37501 gttattccgc acatcactaa cgctatcaaa gagcgcgtgc tggaaggcgg cgaaggtcat
    37561 gacgtagtac tggtcgaaat cggcggtact gtaggtgata tcgaatccct gccgttcctc
    37621 gaagcgattc gccagatggc tgttgaaatt ggccgtgagc acaccctgtt tatgcacctg
    37681 acgctggtgc cgtacatggc agcgtctggt gaagtcaaaa ccaaaccgac tcagcactct
    37741 gtaaaagagc tgctttcaat cggtatccag cctgatatcc tgatttgtcg ttcagatcgc
    37801 gccgttccgg cgaacgaacg tgcgaagatt gcattgttct gtaatgttcc ggaaaaagcg
    37861 gttatttctc tgaaagacgt cgattccatc tataaaattc cgggcctgtt gaaatctcag
    37921 gggctggacg attatatttg taaacgattc agcttaaact gcccggaagc gaatctgtcc
    37981 gaatgggaac aggttatctt cgaagaagcg aatccggtaa gtgaagtcac cattggtatg
    38041 gtcggcaaat acattgagct gccggatgca tacaaatccg tgatcgaagc actgaaacac
    38101 ggtgggctga agaatcgtgt cagcgtcaac atcaaactga tcgattcaca agatgttgaa
    38161 acgcgcggcg ttgaaatcct caaaggtctg gacgcaatcc tcgtacctgg cggtttcggc
    38221 tatcgtggcg tagaaggcat gattacgacc gcgcgttttg cgcgtgagaa caatattcct
    38281 tatctgggca tttgcctggg tatgcaggtg gcgttaattg attacgctcg ccatgttgcc
    38341 aacatggaga acgccaactc tacggaattt gtgccagact gtaagtaccc ggttgtggcg
    38401 ctgattaccg agtggcgcga tgaaaacggc aacgttgaag ttcgtagcga gaagagcgat
    38461 ctcggcggta ccatgcgtct cggcgcacag cagtgccagt tggttgacga tagcctggtt
    38521 cgccagctgt acaatgcgcc gacaattgtt gagcgtcatc gtcaccgtta cgaagtcaac
    38581 aacatgctgt tgaaacagat tgaagatgca ggtctgcgcg ttgcgggccg ttccggggat
    38641 gatcagttgg tcgagatcat cgaagttccg aatcatccgt ggttcgtggc ttgccagttc
    38701 catccggagt ttacttctac tccacgtgat ggtcacccgc tgtttgcagg ctttgtgaaa
    38761 gccgccagcg agttccagaa acgtcaggcg aagtaagtaa aaaagttaga acggcaacgc
    38821 gtaccctggg tacgcgttgt ttgtctggag tttcagttta actagtgact tgaggaaaac
    38881 ctaatgtcca aaatcgtaaa aatcatcggt cgtgaaatca tcgactcccg tggtaacccg
    38941 actgttgaag ccgaagtaca tctggagggt ggtttcgtcg gtatggcagc tgctccgtca
    39001 ggtgcttcta ctggttcccg tgaagctctg gaactgcgcg atggcgacaa atcccgtttc
    39061 ctgggtaaag gcgtaaccaa agctgttgct gcggtaaacg gcccgatcgc tcaggcgctg
    39121 attggcaaag atgctaaaga tcaggctggc attgacaaga tcatgatcga cctggacggc
    39181 accgaaaaca aatccaaatt cggcgcgaac gcaatcctgg ctgtatctct ggctaacgcc
    39241 aaagctgctg cagctgctaa aggtatgccg ctgtacgagc acatcgctga actgaacggc
    39301 actccgggca aatactctat gccggttccg atgatgaaca tcatcaacgg tggtgagcac
    39361 gctgacaaca acgttgatat ccaggaattc atgattcagc cggttggcgc gaaaactgtg
    39421 aaagaagcca tccgcatggg ttctgaagtt ttccatcacc tggcaaaagt tctgaaagcg
    39481 aaaggcatga acactgctgt tggtgacgaa ggtggctatg cgccgaacct gggttccaac
    39541 gctgaagctc tggctgttat cgctgaagct gttaaagctg ctggctatga actgggcaaa
    39601 gacatcactc tggcgatgga ctgcgcagct tctgaattct acaaagacgg taaatacgtt
    39661 ctggctggcg aaggcaacaa agcattcacc tctgaagaat tcactcactt cctggaagaa
    39721 ctgaccaaac agtacccgat cgtttccatc gaagacggtc tggacgaatc tgactgggac
    39781 ggtttcgcat accagaccaa agttctgggc gacaaaatcc agctggttgg tgacgacctg
    39841 ttcgtaacca acaccaagat cctgaaagaa ggtatcgaaa aaggcatcgc taactccatc
    39901 ctgatcaaat tcaaccagat cggttctctg accgaaaccc tggctgctat taagatggcg
    39961 aaagacgctg gctacactgc agttatctct caccgttctg gtgaaactga agatgctacc
    40021 atcgctgacc tggctgttgg tactgctgca ggccagatca aaacgggttc catgagccgt
    40081 tctgaccgtg ttgctaaata caaccagctg attcgtatcg aagaagctct gggcgaaaaa
    40141 gcaccgtaca acggtcgtaa agagatcaaa ggccaggcat aagtctgact ttatctgatt
    40201 taaaaatgcc agccttcggg ctggcatttt ttatggctga gttaccagcg gccagacgcg
    40261 ccgccaccgc cagaagaacc gccacctccg ctaaatccgc cgccacttga gccgccacca
    40321 gaagaggatg aagaagaacc ttttcttgaa gctctttctt tactgagttt tttgtatttg
    40381 gcgcgtagct ttttattgac gataagccaa aaaacaacca gtggtacgat cgcaaaaggc
    40441 gttaacatac cgcaggcaac gattaaattg gtccatggtc ctggctcggc aacatagagg
    40501 aataccatcc cgtaaatggc gccagtgaga gtgatccagg gtaaattcga cggatcggta
    40561 aaaacaataa aatatgaaag taccgccagc aatataatta cagcaagcga taatggaata
    40621 gggggattga tactgtaata atcccccctg tcttccggta attcaccatt caacagaagc
    40681 gcgccgatat tttcgcttgc cagtgttaat ccacccatca ggtcatcact tttgaaggca
    40741 gggatcaata tatccctgat aatcttcgcc gcttgcaggt cggtaaccac cccttccagc
    40801 ccgtagccaa cctcaatacg aacagcatga tcctcccagg caaccaaaag taaaatacca
    40861 tcgtttctct gcttatctcc cagtttccag ctatcaaaaa cccttgtagc atattgttca
    40921 atggaatcgt ctccggtgga cggcactact aatacagcga cctgcgcccg tgttttatgt
    40981 gtaatatcct gcagttgctg tgtcagtgat tgttgttcag aagtgcttaa cgtaccagta
    41041 atatcggtca cctgttggcg cagttccggc acagcaattt gctgggcaag cccaggaagg
    41101 caccacaaag tgaatagaat aaagattaag cgcattacga tcaccaacgg ccggatgaac
    41161 cgccgcctcc tgaagaaccg ccaccaccgc ggaaagaatt tctcgacgaa gagcgagaat
    41221 gagcattacg attccccgtt ttcctgttgc ttttttctcc tgaccccagc ttaagattgc
    41281 cagtaaattt ggccacaatg atcagcagaa cgatgccagc aatggttatt gggccgataa
    41341 aatgatcaat taacccaata attgttgaga tgaatgcaat atctttatta ctggcgaaca
    41401 ccaggcacca tcccacaata attatcagca tcacgacaaa caatgaattt cgtcctgatt
    41461 cacgaacttg cggagtcttc ttttgttttt tcactttagc gtttttattt tttggggcca
    41521 gcatcatagg aaatacgcgg cacatattaa aaactactgc gagacgctgc cagcagattg
    41581 ctgcgaaaaa taaactaaaa caaacgaccg acacccgccg aatgatgaca tcgtcctgaa
    41641 agcagaaaaa ccagaccgta ggtgtaatca ttgatatcat ccatatgcca ggtcctggac
    41701 ggctggtaaa tcgccagagg acaatgatgg caacgatcat cagcactgtc caggcaaggc
    41761 atggcagcga aagttgcgga ttagcaaaca gatgattggc taaagcatcc aaagggctaa
    41821 ggttcgcttt gatatgggta aactgtgcga tgctttcaat tccgcccgtt aatccagcaa
    41881 agagattgtt tttttcgaat tctggctgca tatggtaacg aacaatttgc gcagcttgtt
    41941 ccggcggcaa catctgttcc agtccatgtc caatcttcat tgacgccagc ccttccggcc
    42001 aaacgacgag aattaagata ccttcgccat atcgcttctc tccaacgggt ttccagtgat
    42061 ataccccgta tctctgcgag gctgcaagtt taatgttttt gccccctgtt gtcggcgtaa
    42121 ccaacacggc cacctggaag tggttttgct gctcgagagt ctgtaattgt tgatttaaag
    42181 cactttgttc ttctgtcgtt aatgtcccgg ttaagtcagt cactgaaggc aatcgttcag
    42241 cgaatacctg aaaaccgcat agcatcagaa gtaagagaaa aatacgcata tcaattcctt
    42301 ttgttaccag cgcccggaag cgccaccgcc gccagatgaa ccgccaccac cgctaaaatt
    42361 gtcattgtcg aatgaagagg atgacgatga tgaatagtgg gaggaagaat tgcttcttgt
    42421 atggcgtgat ggctggttgg catcacgcat cgaatcccga acctttttgc cgaagggggt
    42481 aaaagcgatg gcaactgcca gaaacggtgt ggcgaagcag agtaatgttg tgagtactcc
    42541 ccagcttccg ctgaaacctg ctataggtaa aacaaaggcc agtacaatgg ctgcgaagca
    42601 aattactccc aacgttttga tatatcctct ggcagaaata aacgtcagta ttatcgccca
    42661 gactaataaa gcccaccaac cgctaaaggg caaagggtga tcggtcggtg ttatcgttgc
    42721 tgaattattc cccatcaggc gactttcaat atcgttgatc cccttttgta aaccaccagc
    42781 caggtcgcct tttttaaatg ccggaataat actgttgcga ataattttcc cggactgtgc
    42841 atccgtaata ataccttcca gcccgtaacc gatttcaata cgcacggtat gatcctgcca
    42901 ggcgactaac agtagaacgc catcatcacg gtctttatca cccggtttcc agctatcgaa
    42961 tacccgcgtt gcgtactgct cgatggtttc atcgcctgtt gtatcaacca ctaacacgac
    43021 caactgtgcg tggttttttt gcgtcagggc tttaatctgg ttggtcaggg tgtttacttc
    43081 gctgtttgtt aatgtgccgg ttaagtcatt cacatacctg ggtgattcag gtactggaat
    43141 ttgtgtagca aacacagatg aacagaagaa agccataagg aagataaaat ttcgcataga
    43201 acgtccccgc ttaatgagca ttagcattct ggctaaaatc gattgttggg gctttactta
    43261 ctgcggtgac atcatccggc aaataatttt tctttggtgt gtaatccatg acctttgcgg
    43321 ttaatacagc ggggaatttg cggatagtga cattatattg ttcaatggct ttaatatatc
    43381 ttcctcgcgc aacggcaatt ctattttcac ttccttcgag ctgtaccatt aagttttgat
    43441 agagctcttg agcctttaac tgagggtaac gctcactaat aatcgttaac tgcccgagag
    43501 tgcgagtcac ctgcgcctgc gcctgttgcc aggcttgtaa tttttgttca tctcccggag
    43561 ttttctgaag atcgctactg gcgcgattag cctgactacg tgccaatgtt acggcttcta
    43621 gtacctcttg ttcatggctg gagtaacctt taatactagc gacaagatta ggaataagat
    43681 cggtgcgacg ttgatattga tttaatactt ctgaccagga ggcattaacc tgttcatcat
    43741 aggtttgaat atcgttataa ccgcaacctg ataaattgaa aataaatata aacgcgatga
    43801 atattctaaa catgtttaaa tccctttcat ttaatccttg aaaacataat acgccgcttc
    43861 attttattct taatgatctg agccaatttt tgcatcaaac ctgcctgtaa cagaatgtta
    43921 ataagacatg attgttgtgg caggatctgc aataattaac aattatccac ttcacagaga
    43981 atgctatgca gtacccgatt aacgagatgt tccagaccct gcaaggtgag ggttacttta
    44041 ccggcgtccc cgccattttt attcgtttac agggatgtcc ggttggctgt gcctggtgcg
    44101 acaccaaaca cacctgggaa aagcttgagg atcgggaagt ctcccttttc agcattctgg
    44161 cgaagaccaa agagagtgat aagtgggggg cggcgagcag tgaagatttg ctggctgtca
    44221 ttggccgtca gggatacacc gcgcgtcatg tggtgattac gggtggtgag ccttgcattc
    44281 atgatttgct gccactgact gacctgctcg aaaagaacgg ttttagctgc cagatcgaaa
    44341 ccagtggtac tcatgaagta cgctgcacac cgaatacctg ggttaccgta tcaccaaagc
    44401 taaatatgcg cggtggctat gaagtgttgt cacaagccct ggagcgagcc aacgaaatca
    44461 aacacccagt aggacgcgta cgtgatattg aagcactgga tgaattgctg gcgacgctga
    44521 ccgatgataa accgcgagtc attgcacttc agccaattag ccagaaggaa gatgctacgc
    44581 gtttatgtat cgatacctgc attgcgcgca actggcgttt gtcgatgcag acacataaat
    44641 atttgaatat tgcctgatta aacatttata agcgttataa atgggtggaa tctgtaaaga
    44701 cttctactca tttatattct ttgtcgtctc tgaaaaacct ccattttgcc cattctggac
    44761 taatcattat cattctctac aaattctgtg gcgttaattt ttcgctggag tgaaaattat
    44821 tgcggtaaag tttggtagat tttagtttga atagagtcat tgtaaatatt tatcttttta
    44881 at
//
LOCUS       DADTFG010000016        44301 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.016, whole genome shotgun sequence.
ACCESSION   DADTFG010000016 DADTFG010000000
VERSION     DADTFG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.016"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(107..991)
                     /gene="xapR"
                     /locus_tag="J1138_000912"
     CDS             complement(107..991)
                     /gene="xapR"
                     /locus_tag="J1138_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HBA8452748.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNILARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(1032..1190)
                     /locus_tag="J1138_000913"
     CDS             complement(1032..1190)
                     /locus_tag="J1138_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HBA8452749.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1243..2499)
                     /gene="xapB"
                     /locus_tag="J1138_000914"
     CDS             complement(1243..2499)
                     /gene="xapB"
                     /locus_tag="J1138_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HBA8452750.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVIAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(2560..3393)
                     /gene="xapA"
                     /locus_tag="J1138_000915"
     CDS             complement(2560..3393)
                     /gene="xapA"
                     /locus_tag="J1138_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HBA8452751.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            3641..4405
                     /locus_tag="J1138_000916"
     CDS             3641..4405
                     /locus_tag="J1138_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452752.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTSRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(4444..5370)
                     /locus_tag="J1138_000917"
     CDS             complement(4444..5370)
                     /locus_tag="J1138_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8452753.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAVEDYVPWQALQGEKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            5460..6458
                     /locus_tag="J1138_000918"
     CDS             5460..6458
                     /locus_tag="J1138_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBA8452754.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPINVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCSSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(6455..6673)
                     /locus_tag="J1138_000919"
     CDS             complement(6455..6673)
                     /locus_tag="J1138_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452755.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGHRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKNEGLTQLVQPLKRPL"
     gene            complement(6675..8690)
                     /gene="ligA"
                     /locus_tag="J1138_000920"
     CDS             complement(6675..8690)
                     /gene="ligA"
                     /locus_tag="J1138_000920"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBA8452756.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(8761..9759)
                     /gene="zipA"
                     /locus_tag="J1138_000921"
     CDS             complement(8761..9759)
                     /gene="zipA"
                     /locus_tag="J1138_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBA8452757.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9989..10750
                     /gene="cysZ"
                     /locus_tag="J1138_000922"
     CDS             9989..10750
                     /gene="cysZ"
                     /locus_tag="J1138_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBA8452758.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10935..11906
                     /gene="cysK"
                     /locus_tag="J1138_000923"
     CDS             10935..11906
                     /gene="cysK"
                     /locus_tag="J1138_000923"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBA8452759.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            12290..12547
                     /gene="ptsH"
                     /locus_tag="J1138_000924"
     CDS             12290..12547
                     /gene="ptsH"
                     /locus_tag="J1138_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBA8452760.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            12592..14319
                     /gene="ptsI"
                     /locus_tag="J1138_000925"
     CDS             12592..14319
                     /gene="ptsI"
                     /locus_tag="J1138_000925"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBA8452761.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            14360..14869
                     /gene="crr"
                     /locus_tag="J1138_000926"
     CDS             14360..14869
                     /gene="crr"
                     /locus_tag="J1138_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBA8452762.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(14912..15763)
                     /gene="pdxK"
                     /locus_tag="J1138_000927"
                     /gene_synonym="thiJ"
     CDS             complement(14912..15763)
                     /gene="pdxK"
                     /locus_tag="J1138_000927"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBA8452763.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            15868..16236
                     /locus_tag="J1138_000928"
     CDS             15868..16236
                     /locus_tag="J1138_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBA8452764.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(16239..17150)
                     /gene="cysM"
                     /locus_tag="J1138_000929"
     CDS             complement(16239..17150)
                     /gene="cysM"
                     /locus_tag="J1138_000929"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBA8452765.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17285..18382)
                     /gene="cysA"
                     /locus_tag="J1138_000930"
     CDS             complement(17285..18382)
                     /gene="cysA"
                     /locus_tag="J1138_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBA8452766.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(18372..19247)
                     /gene="cysW"
                     /locus_tag="J1138_000931"
     CDS             complement(18372..19247)
                     /gene="cysW"
                     /locus_tag="J1138_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBA8452767.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(19247..20080)
                     /gene="cysT"
                     /locus_tag="J1138_000932"
     CDS             complement(19247..20080)
                     /gene="cysT"
                     /locus_tag="J1138_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBA8452768.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20080..21096)
                     /locus_tag="J1138_000933"
     CDS             complement(20080..21096)
                     /locus_tag="J1138_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8452769.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(21254..22045)
                     /gene="ucpA"
                     /locus_tag="J1138_000934"
     CDS             complement(21254..22045)
                     /gene="ucpA"
                     /locus_tag="J1138_000934"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBA8452770.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            22325..23221
                     /gene="murQ"
                     /locus_tag="J1138_000935"
     CDS             22325..23221
                     /gene="murQ"
                     /locus_tag="J1138_000935"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBA8452771.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            23225..24649
                     /gene="murP"
                     /locus_tag="J1138_000936"
     CDS             23225..24649
                     /gene="murP"
                     /locus_tag="J1138_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBA8452772.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVTADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSEQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(24827..25726)
                     /gene="yfeX"
                     /locus_tag="J1138_000937"
     CDS             complement(24827..25726)
                     /gene="yfeX"
                     /locus_tag="J1138_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBA8452773.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRTLSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(25822..26397)
                     /locus_tag="J1138_000938"
     CDS             complement(25822..26397)
                     /locus_tag="J1138_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBA8452774.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDFYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(26458..26907)
                     /locus_tag="J1138_000939"
     CDS             complement(26458..26907)
                     /locus_tag="J1138_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBA8452775.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(26894..27319)
                     /locus_tag="J1138_000940"
     CDS             complement(26894..27319)
                     /locus_tag="J1138_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HBA8452776.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            27533..28402
                     /gene="amiA"
                     /locus_tag="J1138_000941"
     CDS             27533..28402
                     /gene="amiA"
                     /locus_tag="J1138_000941"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBA8452777.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            28406..29305
                     /gene="hemF"
                     /locus_tag="J1138_000942"
     CDS             28406..29305
                     /gene="hemF"
                     /locus_tag="J1138_000942"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBA8452778.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(29311..30363)
                     /gene="eutR"
                     /locus_tag="J1138_000943"
     CDS             complement(29311..30363)
                     /gene="eutR"
                     /locus_tag="J1138_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBA8452779.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(30409..30909)
                     /gene="eutK"
                     /locus_tag="J1138_000944"
     CDS             complement(30409..30909)
                     /gene="eutK"
                     /locus_tag="J1138_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBA8452780.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(30922..31581)
                     /gene="eutL"
                     /locus_tag="J1138_000945"
     CDS             complement(30922..31581)
                     /gene="eutL"
                     /locus_tag="J1138_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBA8452781.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(31591..32478)
                     /locus_tag="J1138_000946"
     CDS             complement(31591..32478)
                     /locus_tag="J1138_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBA8452782.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(32499..33860)
                     /locus_tag="J1138_000947"
     CDS             complement(32499..33860)
                     /locus_tag="J1138_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBA8452783.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(33872..35275)
                     /gene="eutA"
                     /locus_tag="J1138_000948"
     CDS             complement(33872..35275)
                     /gene="eutA"
                     /locus_tag="J1138_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBA8452784.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILKQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGPGTDARSLTGGQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(35272..36357)
                     /gene="eutH"
                     /locus_tag="J1138_000949"
     CDS             complement(35272..36357)
                     /gene="eutH"
                     /locus_tag="J1138_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBA8452785.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAV
                     LVALGLKFIPEKMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGD
                     KPGEVMRAIEVIGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATL
                     ANNIPMFGMMKQMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGG
                     VTAIGVAMMLVPKEDASAAKTEAEAQS"
     gene            complement(36574..37761)
                     /gene="eutG"
                     /locus_tag="J1138_000950"
     CDS             complement(36574..37761)
                     /gene="eutG"
                     /locus_tag="J1138_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBA8452786.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSIPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(37751..38587)
                     /gene="eutJ"
                     /locus_tag="J1138_000951"
     CDS             complement(37751..38587)
                     /gene="eutJ"
                     /locus_tag="J1138_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBA8452787.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            complement(38598..40001)
                     /locus_tag="J1138_000952"
     CDS             complement(38598..40001)
                     /locus_tag="J1138_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBA8452788.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTQLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(40013..40300)
                     /gene="eutN"
                     /locus_tag="J1138_000953"
     CDS             complement(40013..40300)
                     /gene="eutN"
                     /locus_tag="J1138_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBA8452789.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(40407..40700)
                     /gene="eutM"
                     /locus_tag="J1138_000954"
     CDS             complement(40407..40700)
                     /gene="eutM"
                     /locus_tag="J1138_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBA8452790.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(40739..41755)
                     /gene="pta"
                     /locus_tag="J1138_000955"
     CDS             complement(40739..41755)
                     /gene="pta"
                     /locus_tag="J1138_000955"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBA8452791.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(41752..42555)
                     /gene="eutT"
                     /locus_tag="J1138_000956"
     CDS             complement(41752..42555)
                     /gene="eutT"
                     /locus_tag="J1138_000956"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBA8452792.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLAC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(42552..43253)
                     /gene="eutQ"
                     /locus_tag="J1138_000957"
     CDS             complement(42552..43253)
                     /gene="eutQ"
                     /locus_tag="J1138_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBA8452793.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(43228..43707)
                     /gene="eutP"
                     /locus_tag="J1138_000958"
     CDS             complement(43228..43707)
                     /gene="eutP"
                     /locus_tag="J1138_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBA8452794.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQTVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(43720..44055)
                     /gene="eutS"
                     /locus_tag="J1138_000959"
     CDS             complement(43720..44055)
                     /gene="eutS"
                     /locus_tag="J1138_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBA8452795.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        10426 a        12356 c        11173 g        10346 t
ORIGIN      
        1 aaaaaagcgc cctaaaggcg cttttttgct atctgcaatt tgcgaaattg cctgatgcgc
       61 ttcgcttatc agaccaacta tgtccggcag ttcccgtgcc tctcatctac tgcgccaacg
      121 ccgccagcag tttaaccagc gcgggcgtca cctgctgcgt ttcataaaca atatataagt
      181 ctgccgggat gcgttctttg agcggacgga atatgacacc gggccagttc atctgcgcgt
      241 agctgtccgc tatcaatgtg ataccaatac ccatactgac catggcgagt accgtttgcg
      301 gttcattaac ttcgcgaata acaaccggtg aaaatcccac ctgctggcaa actcgctgca
      361 aaaaatccca gtcagtgtac acgggcggca ttgtaacgaa atactcgtca cgtagcgctt
      421 ccagcgggac ggtggaaaat gatgagagat gatgctcttc aggcatcgcc accagaaacg
      481 ccgattcatg caaccgtaag ctggtaaaac cagtcggtgg ttctgtcgcc attcgccaga
      541 tcccggcatc aagttcgcgg cgttccagca aggccatttg catcgctggc atcttttcgc
      601 gaaacagaac ttcaacgtta ggattttctc taaggaatcg ccgcataacc gggcgcatcc
      661 gtccccacat tgccgttccc acaaccccga gttcaatccg ccctgcttct ccccgaccta
      721 tttgttcaac ccgagccaat atattatttg cattcaccag caatcgacgc gattcttcca
      781 tcaagatttt gcccgcgtgt gtcagtacga cgctgcgcga atggcgaata aaaagctgcg
      841 tgccgagttg attttccagc tctttaatat gaatactgag cggaggctga gacatattta
      901 aacgcgctgc tgcgcgacca aaatgcaact cttccgctac ggcaagaaaa taacggagca
      961 acttaagatc tgttctatat acgcgttcca taattaagtg ctccatataa aaaatatatc
     1021 gtattaaatt atcatggcat aaagtatttc accaccagat atccgacata cataaacacg
     1081 atggataata tacccggcaa tacatttgga acaggaatag gtaatcgcac gattgtaaac
     1141 aaagcaccaa tgccaaaacc aacgccagaa gaaaataaaa tatctttcac taattaacct
     1201 ttatcataaa agcagcccca aggaacagag ctgcgaatcc ttttaatgtg taactgaacg
     1261 atgcgttatc ttttcagggt catggttata tttaaaccca aagaaaaata tcactgcgag
     1321 gaaaagagca taccctgcaa acatcagcca aatggtatgc caatctttta cgccatccac
     1381 cgagaagtaa tccactgcca taccgctcag aatcgagcca acccatgcgc cgacgccatt
     1441 taccatcgtc ataaaaaggc cctgggcgct ggcacgaatg ctggaatcaa cttcctgttc
     1501 gacaaagacc gaaccagaaa tattgaagaa atcgaatgca caaccgtaaa caatcatcga
     1561 cagcaacagc aaaataaacc cggttggtga tggatcgcca taggcaaaga agccaaagcg
     1621 cagcgtccac gccaccatac tcatcagcat gacggtttta ataccgaaac gcttcaaaaa
     1681 gaacggtatc gtcagaatga aaccgacttc agccatctgt gaaactgaca gtaaaatgga
     1741 cgggtatttc accacgaagc tatcagcaaa ctcaggatta cgggcgaaat catgcaggaa
     1801 tgggttgcca aaaacgttgg taatttgcag caccgcgccc agcatcatgg cgaagaggaa
     1861 aaagatagcc atgcgtggat ttttaaacag cacgaaggca tccagaccca gcttgctggc
     1921 aagcgatgta gtcgcttttt tctccgcaac cggaatcttc ggcaaagtca acgcataagc
     1981 cgacagcagc aatgacgcac cggacgcgat atacagttgc agactactca attccagatg
     2041 cagcaggctt actgcccaca tggcgacaat gaaccccacc gtaccaaaaa cgcgaatggg
     2101 cgggaaagcg atcaccgggt caagccctgc ctgggcaaga caggaataag agacgctgtt
     2161 cgataacgca atagtcggca taaacgccat cgcattgact aacatcaccc aaaacatcat
     2221 atccggatca gttacggatg ccgcataaaa aagtacgccc gcacacacca ggtggcacag
     2281 catgtatgca cgttctgcac gcagccattt gtctgcgatg atccccatta tacccggcat
     2341 aataatcgcg gcgatccctt tggaactgta aaccatgcca acattagcgc cggtgaaatg
     2401 aagggtatta atcatgtaag aaccgagggt aaccagccag ctcccccaga taaaatattg
     2461 caaaaaggac attaccttta agcgcaaagc gatgctcata atcgtttcct tgtcgcatca
     2521 tatggcctca ctcagtgagg ccgtttattg ttgttttact caggcaattt tgcgcagaaa
     2581 gccgcaaata agattaataa agttttgctt tgagagttcc gctgctgcca gcgtttgggc
     2641 atgagaaagc ttcacgtcac tcagaccttc cgccatgttg gtaatcgcag agaccgcaac
     2701 gactttaagt tcgcaatggc gagctgaaat aacctcaggc accacagaca taccaacaac
     2761 atccccacca ataatttgca tcatgcgaat ttccgccgca gtctcgaaat tcggccccgg
     2821 atacgagacg aacacgccct ccgtcagagg gaacccctct tctttcgcca ctttttgtaa
     2881 cagtgcgcgg tattccgcat cgtaggcatt agccagcgag aagaagcgct caccaaaacg
     2941 ttcatcatta agacccacca tcggcgttcc cggcatggtg ttgatgtgat ctttcaatgc
     3001 gaccagactg ccggccccca cttcaggacg cagtgagcca gccgcattgg tgcagaacag
     3061 caactcgcag cccagcaact taaaggtacg gattgcatcc gtcatgatgg tcatcccacg
     3121 accttcgtag aaatgtccgc gacctttcat gcacgccact ggcaccccct ggagataccc
     3181 cagcaccagc tcacccgcat gaccgtgtac ggtacttacc gggaacccag gcagcttttc
     3241 gtaggaaatt gcgaccgcgt tctcaatctg atcggccagc gcgcccagcc cggaacctaa
     3301 aataaaggcc actcgtggcg tgaaatcagg tttataagtc ttgataatat ctacgcaata
     3361 cagtgggtta tgagaaaact gagcctgata catatatatc ctttttttgt agggtggaat
     3421 ctaacgcctt tttataacga gattgcccta cctcatacca atacactttt ttcatgtatc
     3481 cgatagttat tcgatatcga tatatttata ttttttcact attaataggc tgaaaaaact
     3541 gtattggtac tctcctttcc cttgcattaa caatatgccg cttttaatca accttttatt
     3601 aagcaatcaa ctaataacga tcggcatagg gaattatgct atgaaaaaac atctttttac
     3661 tctgacactc tcttctgtat tggcgatacc agcagtatcc catgcagaat ttaaaggcgg
     3721 ttttgctgat ataggtattc attatcttga ctggactagc agaaccactg agaaatcgtc
     3781 gaccaaatca cacaaagatg attttggata tctggaactg gaaggcggcg ctaactttag
     3841 ttggggagaa atgtacgggt tcttcgactg ggaaaacttt tataatgatc gtcatgataa
     3901 accgggtagc gagcagcgtt atacatttaa aaataccagc cgtatttacc tgggtgacac
     3961 cggatttaat ctctacttgc atgcgtatgg cacctacggt tctgcgaatc gtgtgaattt
     4021 ccatgacgat atgttccttt acgggatcgg ctacaatttc accggcagcg gttggtggtt
     4081 caaaccgttc tttgcaaaac gctatacaga ccaaacctat tataccggcg ataacggtta
     4141 tgtcgccggt tgggttgcag gttacagctt tatgctgggc agtgagaaat tcaccctcac
     4201 caactggaac gagtacgagt ttgatcgtga tgccacctac gcggcaggta atggcggcaa
     4261 agaaggtctg aacggtgccg tcgcactttg gtggaatgca acttcacaca tcaccacagg
     4321 gattcagtat cgttatgcgg atgacaaact gggcgaagat ttttaccagg atgcgataat
     4381 ctactccatc aaatttaatt tctgattgat tactgccgga gctcccggca gtatgaattc
     4441 actctatatc tgatacaacg gatccccttc ccgcccagcc attagcgcat tgccctggtc
     4501 tcgtactacg tcccataacg cttcagcggc ggtggaaagt gaacggttct tcctgcgcac
     4561 cagcatcaac tggcgttcaa ccactggcgt aatgcgcttt accaccagtg ggctaccttc
     4621 aggcaatggt agcgccagtg cgggaagaat actgatgccg atgcctgcgg ctaccatcgg
     4681 gaacagcgtc gccggatgac caatctcctg cacaatattc gcctgaatac cgttgcgcgc
     4741 cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttctc
     4801 tccctgtaat gcctgccagg ggacataatc ttccacggcc aaagcgctat cgcgatggca
     4861 aagcagaaag aaaggctcgg aaagaatcac ttcacattgc agatcgccca ctgggccagg
     4921 atcgatgacg atgccaaaat cgacatctcc ctggcgaata ctttccatca cccactgctg
     4981 cggacgatcg tgcaggacaa actgaatatc tggatagcgg cgatggcttt cagcaatgca
     5041 ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt ttccgctcag
     5101 ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca gcagtcgctc
     5161 aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg tggttctgtc
     5221 cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactca ctgccgactg
     5281 gctcaggccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa
     5341 aacctttaat tgctttaaag aataattcat ctgttttttt catgaatgga tgcaataaat
     5401 caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct ggagtcttta
     5461 tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg gtgttgctgg
     5521 catctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg accactgcag
     5581 cgattgctct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg attattgctg
     5641 gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg ctgtttccga
     5701 ttctgggcgt gctgtttgcc tggtggaaac cgataaatgt cgacccgatg ctctactccg
     5761 gttttctcta cttgtgcatt ctccccgcga ccgtgcagtc tgcaatcgcc ttcacgtcaa
     5821 tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc ctgctgggga
     5881 ttttcctttc accattgctg gttggtctgg tgatgaatgt tcacggtgca gggggcagcc
     5941 ttgagcaggt cggtaaaatt atgctgcaac tgttgctgcc atttgtgttg gggcatcttt
     6001 cccggccttg gattggtgac tgggtgtcgc gtaataaaaa atggattgcg aaaactgacc
     6061 agacgtccat tctgttggtg gtttatacgg cattcagcga agccgtcgtt aacggtatct
     6121 ggcacaaagt tggctgggga tcattgctgt ttatcgtggt ggtcagttgc gttcttctgg
     6181 ctatcgtgat tgtagttaac gtctttatgg cacgccgact gggcttcaat aaggcagatg
     6241 aaattactat cgtcttttgt agttcgaaaa agagtctggc aaatggcatc ccgatggcaa
     6301 acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg attttccatc
     6361 agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag accgaacagt
     6421 tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc aggggctgga
     6481 ccagttgcgt cagcccctcg tttttaatca gcagcgtgat ttgcattagc tcaccgagct
     6541 tccctgcagg gaactcatct ttgcgggcaa accacagcag atactcctcc ggcaggtcga
     6601 ttaagcgatg ccctttgtat ttgccaaacg gcattatcgt attggctatt tcaatcagct
     6661 gctctttttc catctcagct acccagcaaa cgcagcattt ccgtttcgtc gatgacttca
     6721 atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc tatcaccaga
     6781 tcggttttct tcgacacgct gcccgcgact ttcgccccca gttcgaccag tcgagcttta
     6841 gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt accagcaaac
     6901 ggactgtcaa tctcttccgc attgataacg atcggctcag gccagtgaac accttccgcc
     6961 aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg aacgtgggag
     7021 gcaacgacaa ttccaacatc aggcaccttt tgcagctctt caatcgacgc ggcttccagc
     7081 gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tagcctcgcc gacttcacgg
     7141 atgccaagtg catagaggaa gcgggcaaag gtggtttctt tcgctttttc cagcgcgtta
     7201 accacgtttt gtgccgattt cggccccata cgctccagtc cggtcagttt gcctgcggtg
     7261 agtttgaaca gatccgccgg agtgtggaca tattcttttt caaccagttg atcgatgatt
     7321 ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt cagagactct
     7381 ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc accttccaca
     7441 cgctcgacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc acgggtatct
     7501 tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc agcgcggcga
     7561 ataaccactt tatcgccaat gcgtaaacca agacgttcga tttcgtcagc attatgcagt
     7621 gtagcgttac tcaccaacac gccagcaaca tggacaggtt ccagacgcgc aacaggcgta
     7681 atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat ctgttcctgc
     7741 gccgggaatt taaacgctac cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc
     7801 tgctgtgcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc cagcgtcgga
     7861 cggtcttctt ctactttgtg gtagaacgcc agcacttctt ccgccgattc acaaagcgtt
     7921 acccgatcgc tgaccggcaa accccacttt ttaaattgca gtaaacggcc aagatgagta
     7981 tccggcagct cgccaccttc cagaacaccg acgccatagc agaagaaagt gagcggtcgc
     8041 ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt acgtgggtta
     8101 gcaaacactt tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc
     8161 ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctcacc gtgtagcttc
     8221 agcggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt ggtgccatcg
     8281 ccacgagtcg cggcactgac taaaacgcca ttttcataca gaatactgac ggcaagacca
     8341 tccagcttca gctcacagca ccaggtgact ttctcgttgc ttttcagacg gtcctgcaca
     8401 cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt tatccagcga cagcattggc
     8461 acttcatggc ggatctggct gaaagccgcc agcggcgctg cacctacacg ttgggtaggc
     8521 gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc gcgcatcaac
     8581 ctgtcgtatt ccgcgtcggg aatttccggc gcatccatca catgataaag atattcatga
     8641 tggcgaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat atcgcaccat
     8701 caatgctaaa aacccccgac aagcgggggt tcgaaaagga gttaatttgc cttaagtgta
     8761 tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca atttctgcgg
     8821 agtcatcata cggcgctgat cgtcaagcac tacaccgccc acttcatcgg caatatgctg
     8881 cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg gtacctgcat
     8941 aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg ttcccggttt
     9001 caccatattc gccaggctaa ataacgccgg gccgctgcca tccgggctaa gatgacgatg
     9061 gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa gaagcagttc
     9121 accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg cttctttgcg
     9181 cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg gtgccgctac
     9241 aggctctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca ctggctgcgg
     9301 cgctgatgcg acctgtggcg aaactggctg ctgcaacggc tgttcaggct gcggctgata
     9361 ggcaggctgc tgcacgggtt gctgcaccgg ctgcgctgga cgcggagcat gttgcggcgg
     9421 tacctgagct tcaggcggct gctggaccgg ttgacgcggc tgcgcagacg cataaggcgg
     9481 ttggtactgg tgttgcggcg acggacgagc agcctcatgc tcctgggcgt tagccggggc
     9541 atgattcacg cggtgaactc gaacctcacc aacgccctca tcatcttcga catcctcgtc
     9601 ataagaatcg tcgtcacgtt ttgacttcat tcgttttaat ggccgatcgc ggaacataga
     9661 agatcgttct ttacggctgg tccagaaacc atgtaccagt aaagcgatta tggcgatcgc
     9721 gccaacaatg attaatatca gacgcaaatc ctgcatcatt atattctctg ttgttctaac
     9781 accttgccac cacggcaaac atttactcac taagagtatt tgccgattac ctcaagtgca
     9841 agtgcactat taactttcac agcacaaaga tagatgaaat cgtgcttttt gctgtttttt
     9901 cgaacatatc ctaactgtcc attgcgcaat tacccggtaa aatacgcaga attttcctgg
     9961 gattggtcaa aaggagctca tcctgattat ggtttcatca ttcacatctg ccccacgcag
    10021 cggtttttac tattttgcgc aaggctggaa gctcgtctcg caacctggga ttcggcgttt
    10081 cgttatttta ccgctgctgg tcaatatttt gttgatgggg ggcgcattct ggtggctctt
    10141 tacgcaactc gatgtctgga tcccgacgct catgagttat gttccggact ggttacaatg
    10201 gctgagctat ctgttgtggc ccctggctgt catctctgtg ctgttagtgt ttggctattt
    10261 cttctcaacg attgctaact ggattgctgc tccgttcaac ggtttattgg ctgaacaact
    10321 ggaagcaaga ctgactggcg ctacaccgcc agataccgga attttcggta tcatgaaaga
    10381 tgtgccgcga atcatgaaac gcgaatggca aaaatttgcc tggtatctgc cgcgcgcaat
    10441 tgtattgcta attctttact tcatccctgg cattgggcaa accgtcgcgc cggtactgtg
    10501 gttcctgttt agcgcctgga tgttagccat ccagtattgt gattacccct tcgataacca
    10561 caaagtgccg tttaaagaga tgcgcaccgc cctgcgcaca cgcaaaatca ccaatatgca
    10621 gtttggtgct ttaaccagcc tgtttacgat gatcccgctg cttaatctgt tcattatgcc
    10681 cgttgccgtt tgtggcgcga cggcgatgtg ggtcgattgc tatcgcgata aacacgccat
    10741 gtggcggtaa caatctaccg gttattttgt aaaccgtttg tgtgaaacag gggtggctta
    10801 tgccgcccct tattccatct tgcatgtcat tatttccctt ctgcatatag atatgctaaa
    10861 tccttacttc cgcatattct ctgagcgggt atgctacctg ttgtatccca atttcataca
    10921 gttaaggaca ggccatgagt aagatttttg aagataactc gctgactatc ggtcacacgc
    10981 cgctggttcg cctgaatcgc atcggtaacg gacgcattct ggcgaaggtg gaatctcgta
    11041 accccagctt cagcgttaag tgccgtatcg gtgccaacat gatttgggat gccgaaaagc
    11101 gcggcgtgct gaaaccaggc gttgaactgg ttgaaccaac cagcggtaat accgggattg
    11161 cactggccta tgtagctgcc gctcgcggtt acaaacttac cctgaccatg ccagaaacca
    11221 tgagtattga acgccgcaaa ctgctgaaag cgttaggtgc aaacctggtg ctgaccgaag
    11281 gtgctaaagg catgaaaggt gcaatccaaa aagcagaaga aattgtcgcc agcaatccag
    11341 agaaatacct gctgctgcaa caattcagca atccggcaaa ccctgaaatt catgaaaaaa
    11401 ccaccggtcc ggagatctgg gaagataccg acggtcaggt tgatgtattt attgctggcg
    11461 ttgggactgg cggtacgctg actggcgtca gccgctacat taaaggcacc aaaggcaaga
    11521 ccgatcttat ctctgtcgcc gttgagccaa ccgattctcc agttatcgcc caggcgctgg
    11581 caggtgaaga gattaaacct ggtccgcata aaattcaggg tattggcgct ggttttatcc
    11641 cggctaacct tgatctcaag ctggtcgaca aagtcattgg catcaccaat gaagaagcca
    11701 tttctaccgc acgtcgtctg atggaagaag aaggcattct tgctggtatc tcttctggag
    11761 cagctgttgc tgcggcgttg aaactacaag aagatgaaag ctttaccaat aagaatattg
    11821 tggttattct gccgtcatcg ggtgagcgtt atttaagcac tgcattgttt gccgatcttt
    11881 tcactgagaa agaactgcaa cagtaatgcc agcttgtaaa aaatgcgtaa aaaagcacct
    11941 ttttaggtgc ttttttgtgg cctgcttcaa actttcgccc ctcctggcat tgattcagcc
    12001 tgtcggaact ggtatttaac cagactaatt attttgatgc gcgaaattaa tcgttacagg
    12061 aaaagccaaa gctgaatcga ttttatgatt tggttcaatt cttcctttag cggcataatg
    12121 tttaatgacg tacgaaacgt cagcggtcaa cacccgccag caatggactg tattacgctc
    12181 ttcgtgcgtc gcgtctgtta aaaactggcg ctaacaatac aggctaaagt cgaaccgcca
    12241 ggctagactt tagttccaca acactaaacc tataagttgg ggaaatacaa tgttccagca
    12301 agaagttacc attaccgctc cgaacggtct gcacacccgc cctgctgccc agtttgtaaa
    12361 agaagctaag ggcttcactt ctgaaattac tgtgacttcc aacggcaaaa gcgccagcgc
    12421 gaaaagcctg tttaaactgc agactctggg cctgactcaa ggtaccgttg tgactatctc
    12481 cgcagaaggc gaagacgagc agaaagcggt tgaacatctg gttaaactga tggcggaact
    12541 cgagtaattt cccgggttct tttaaaaatc agtcacaagt aaggtagggt tatgatttca
    12601 ggcattttag catccccggg tatcgctttc ggtaaagctc tgcttctgaa agaagacgaa
    12661 attgtcattg accggaaaaa aatttctgcc gaccaggttg atcaggaagt tgaacgtttt
    12721 ctgagcggtc gtgccaaggc atcagcccag ctggaaacga tcaaaacgaa agctggtgaa
    12781 acgttcggtg aagaaaaaga agccatcttt gaagggcata ttatgctgct cgaagatgag
    12841 gagctggagc aggaaatcat agccctgatt aaagataagc acatgacagc tgacgcagct
    12901 gctcatgaag ttatcgaagg tcaggcttct gccctggaag agctggatga tgaatacctg
    12961 aaagaacgtg cggctgacgt acgtgatatc ggtaagcgcc tgctgcgcaa catcctgggc
    13021 ctgaagatta tcgacctgag cgccattcag gatgaagtca ttctggttgc cgctgacctg
    13081 acgccgtccg aaaccgcaca gctgaacctg aagaaggtgc tgggtttcat caccgacgcg
    13141 ggtggccgta cttcccacac ctctatcatg gcgcgttctc tggaactgcc tgctatcgtg
    13201 ggtaccggta gcgtcacctc tcaggtgaaa aatgacgact atctgattct ggatgccgta
    13261 aataatcagg tttacgtcaa tccaaccaac gaagttattg ataaaatgcg cgctgttcag
    13321 gagcaagtgg cttctgaaaa agcagagctt gctaaactga aggatctacc ggctattacg
    13381 ctggacggtc accaggtaga agtgtgcgct aacattggta cggttcgtga cgttgaaggt
    13441 gcagagcgta acggcgctga aggcgttggt ctgtatcgta ctgagttcct gttcatggac
    13501 cgcgacgcgc tgcccactga agaagaacag tttgctgctt acaaagcagt ggctgaagcg
    13561 tgtggctctc aggcggttat cgttcgtacc atggacatcg gcggcgacaa agagctgcca
    13621 tacatgaact tcccgaaaga agagaacccg ttcctcggct ggcgcgctat ccgtatcgcg
    13681 atggatcgta aagagatcct gcgcgatcag ctccgcgcta tcctgcgtgc ctctgctttc
    13741 ggtaaattgc gcatcatgtt cccgatgatc atctctgttg aagaagtgcg tgcactgcgc
    13801 aaagagatcg aaatctacaa acaggaactg cgcgacgaag gtaaagcgtt tgacgagtca
    13861 attgaaatcg gcgtaatggt ggaaacaccg gctgccgcaa caattgcacg tcatttagcc
    13921 aaagaagttg atttctttag tatcggcacc aatgatttaa cgcagtacac tctggcagtt
    13981 gaccgtggta atgatatgat ttcacacctt taccagccaa tgtcaccgtc cgtgctgaac
    14041 ttgatcaagc aagttattga tgcttctcat gctgaaggca aatggactgg catgtgtggt
    14101 gagcttgctg gcgatgaacg tgctacactt ctgttgctgg ggatgggtct ggacgaattc
    14161 tctatgagcg ccatttctat cccgcgcatt aagaagatta tccgtaacac gaacttcgaa
    14221 gatgcgaagg tgttagcaga gcaggctctt gctcaaccga caacggacga gttaatgacg
    14281 ctggttaaca agttcattga agaaaaaaca atctgctaat ccacgagatg cggcccaatt
    14341 tactgcttag gagaagatca tgggtttgtt cgataaactg aaatctctgg tttccgacga
    14401 caagaaggat accggaacta ttgagatcat tgctccgctc tctggcgaga tcgtcaatat
    14461 cgaagacgtg ccggatgtcg tttttgcgga aaaaatcgtt ggtgatggca ttgctatcaa
    14521 accaacgggt aacaaaatgg tcgcgcctgt agacggcacc attggtaaaa tctttgaaac
    14581 caaccacgcg ttctctatcg aatctgatag cggcgttgag ctgttcgtcc acttcggtat
    14641 cgacaccgtt gaactgaaag gcgaaggctt caagcgtatt gctgaagaag gtcagcgcgt
    14701 gaaagttggt gatactgtca ttgaatttga tctgccgctg ctggaagaga aagccaagtc
    14761 aaccctgact ccggttgtta tctccaacat ggacgaaatc aaagaactga tcaaactgtc
    14821 cggtagcgta accgtgggtg aaaccccggt tatccgcatc aagaagtaat tcttgccgca
    14881 gtgaaaaatg gcgcccatcg gcgccatttt tttatgcttc agccagaggc ggcaaaatca
    14941 attcatcgct ctcatgctgc tgggtatagc gcatcacttc cagtacgcgc aaccccgcgc
    15001 ggtgcaccgc atcggttaac gccttccctt tcagcaatcc actgataagc tgagcacaaa
    15061 acaggtcgcc agtccctttc aggtcagttt taacccggga atgggaaatg acattcacgc
    15121 tgtcggcact gaccaccacc acctgcatct cctgattttc ttcattgccg gaggcgctgg
    15181 taatcaccac ccactttaat gtgtctgaaa gcagactttt tgcggcagca atggcactgt
    15241 cgagatcgcg gcaatcttta ccggtcagga tttccagctc aaagatattg ggggtaattc
    15301 cctgtgccag cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt ttgacataaa
    15361 ttccgctatc aatatcgcca atcaccggat cgaccatgat caataggtca ggatggtctt
    15421 tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt cccatatagc
    15481 ccgtggttac agcacgaagt tggcgcagcg catcacgctc ctgaagcgca cgcaaataac
    15541 cgctaaacca ttcatccgga atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc
    15601 tcagcaatac cgtcggcacg gcaaagacat tcaggccgtt ctgctttata gcaggcaccg
    15661 caatgctgtt gcctacgctg ccgtacacta cctgcgactg cacggcgacg atatccgcct
    15721 gcagtgccct gctcttatcg ttaaacaaca acaaactact catttaattt tttctccttg
    15781 ccgatgatcc tcatcgtatt ccagccgaaa ctttacctga ttctggcagt caaatcggct
    15841 attacaaaac aaggataagg taattcagtg aagaaaatca tttgtctggt cgttacgctg
    15901 ctaatgacac tccccgctta tgcgaagtta acggcccatg aagaagcccg tatcaacgcc
    15961 atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat
    16021 acctgcgatg aagcggtttc tcatctgcgc ctgaagctgg gcaatacccg taatcgcatt
    16081 gatacagccg agcagtttat tgacaaggtg gcctcgtcgt catcgattac cgggaagccg
    16141 tatatcgtga agatgcccgg taagagcgat gagaacgcac agcctttttt acatgcgtta
    16201 attgcgcaga cggataaaac ggtgcctgcg caataatctt aaatccccgc cccctggcta
    16261 aaatgctctt ccccaaacac cccggtagaa aggtagcgat cgccacgatc gcagatgatc
    16321 gccaccacca ccgcgccagg gttagctttt gccacccgca gtgctccggc aaccgcgccg
    16381 ccggagctta cgccgcagaa aatcccttcc cgcactgcca gttcgcgcat ggtgttttcc
    16441 gcatcgcgct gatgaatatc cagcacctca tccaccagag aagcattgaa aatccccggc
    16501 agatattccg caggccagcg gcgaatgccg ggaatactgc tgccttcttc cggctgcagg
    16561 ccaacaatgg tcaccggttt ggattgttcg cgcataaagc gtgacacgcc ggtgatagtg
    16621 ccggttgttc ccatgctgga gacaaaatgg gtaatacgcc cgccagtttg ctgccagatt
    16681 tccggcccgg tagtggtgta atgggcgtaa gggttatcgg gattattgaa ctgatcgagc
    16741 aactttcctt cgccgcgatt cgccatttca agcgccagat cgcgtgcgcc ttccatgccc
    16801 tgttctttgg tgaccagaat cagttctgca ccgtaggcac gcatcgccgc acggcgttcc
    16861 tggctcatgt tgtcgggcat cagcaatttc atgcgatagc ctttaagcgc cgcaatcatt
    16921 gccagcgcaa tgccggtgtt accactggtg gcttcgatca gcacatcacc cggtttaatc
    16981 tcgccgcgct tttccgcctc gacaatcatc gaaagcgccg cacggtcttt taccgatccc
    17041 gccgggttat tgccttccag ttttaaccac acttcactgc cgttatccgg cgccattcgc
    17101 tgcaacttca ccagaggcgt attgcctatt gtttgttcta atgtactcac gatctctgtc
    17161 catacgtggt gttgcctgat gcgacgctta cgcgtcttat cagacctaca ggttacaaaa
    17221 ccttgccatt aaaaagcccg gatcgcggga agcgcctccg ggcgtttaac attcactcaa
    17281 cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg ggtttcaata cgctcatcgc
    17341 cgttatacag ccgcgcatgt tgcagaccaa cgtataaacg atcgccacgc tgcggggcat
    17401 cgtcgccatg catcacgacc gtcagcggtt cgttgtacca ccccagcggc tgcaccacta
    17461 attgggtgta gtgacctttc gggctggctt ccagtacctg tactggtagc ggcgaatcga
    17521 ggctggtacg gcggctgata tccacttccc acgggcgcag gaagagatcc accggcccct
    17581 gatacgcggg ggtgtatccc agcggccagc gatgtgcgcc aacgtggaac tgcccgccgc
    17641 gaatggttcc ctgcaggcgg tttacctcgc ccataaactc cagcacaaaa cgagtcgccg
    17701 gttcacgcca tacctgatcc ggtgcgtcag cttgttcaat attgccctga ctcatcacca
    17761 ctacacgatc agctacttcg gtcgcctctt cctggtcgtg ggtcacgaac acgctggtga
    17821 atttcagttc ttcatgcagt tgacgcagcc agcgacgcag ctctttacgc acctgcgcat
    17881 ccagcgcgcc aaacggttca tcaagcagca aaatttgcgg ttccacagcc agcgcgcgtg
    17941 caagcgccac acgctgtttc tggccgccgg aaagctgcgc cggataacga tccgccagat
    18001 gcgcgagctg caccatctcc agcaattttg tcactttcgc tttgatcgcc gccgcattcg
    18061 ggcgttcacg ccgcggcaac accgtcaggc caaaagcgat attgtcgaac accgtcatat
    18121 gacggaacag cgcgtaatgc tggaacacga aaccgacttt acggtcccgc gcgtgcagac
    18181 ggctcacgtc ggtaccgtgg aagcgaatat gcccgctggt ttgatgctcc agcccggcaa
    18241 taatgcgcag cagcgtggtt tttccggaac cggacggccc cagcaacgcg accatctgac
    18301 cagaaggaat atccagtgag atatcgttca gcacctgggt gcgaccaaac gacttcttaa
    18361 tattggcaat ctcaatgctc atgatgttcc tcctgctgcg cgcgtttttc ctggttctcc
    18421 aggcgccact gcaacatact ctttaaaaac agggtgataa tcgccatcag cgtcaacaac
    18481 gcggcggcgg taaaggagcc gacggtgttg tagtcctgct ccagcaattc aatctgtaac
    18541 ggcagcgaca gggtttcgcc acgaatcgag ccggaaacca ccgataccgc gccaaactcg
    18601 ccaatcgcgc gggcgttggt cagcaccacg ccgtaaagca gcgcccagcg gatgttcggc
    18661 agcgtgacgc gacggaacat ctgccagccg gacgcgccaa gtaaaatcgc cgcttcgtct
    18721 tcctggctgc cctgacttaa cattaccggt accagttcgc gcaccacaaa cggacacgtc
    18781 acgaagatgg tgaccagcac catacccggc caggagaaca taatttgcag attatgttca
    18841 tccagccagc cgcccagcgg gccgttagag ccgtagaaca gcagatacac cagaccagca
    18901 accaccggcg acacggcaaa cggaatgtcc agcagcgtca gcaatagctg gcgtccgggg
    18961 aagttaaagc gcgtcaccag ccaggccagc agaatgccga acaccaggtt taccggtacg
    19021 gcaatcagcg cgatcatcac cgtcagccag atggcgtgca gcatgtccgg atcggccaga
    19081 ttttgtaaca ccggcatcag tcctttgctg aacgcctgta cgaagatata aatcatcggc
    19141 accagcagga tgaacgccga taccagcatc ccgatgccaa tcagaaacca tttgccccag
    19201 ttaatcgggc gcgcgtcata acgcttcaat tgtgtcactt ccgccattaa tgacctacca
    19261 cacgccgacc aaagcgactt tgcagagtgt taattgagaa cagcaacagc agcgatgccg
    19321 cgaggatcac cgaggcaatc gcgctcgccg ccgggtaatc aaactcctgt aagcgaacaa
    19381 agatcatcag cgacgtcact tctgttttcc aggcgatatt cccggcgata aaaatcaccg
    19441 cgccaaactc accgaggctg cgggtaaacg acagcgccac acccgccacc agcgccggag
    19501 atagttccgg cagcaccact ttgcgaaaac tctgccagcg tgttgcgccg agcgtttctg
    19561 ccgcctcttc atattccggg cctaactctt ccagcaccgg ctgcacggtg cgcaccacaa
    19621 acggaatgct ggtaaaggcc atcgccaccg caatccccag ccaggtatag gtgactttga
    19681 tatcaaactt cgccagccat tcaccgtaaa aaccgttcac ggaaaagagc gaggccagcg
    19741 ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg
    19801 tgcgacccgg aaagcgatag cgggttaaga tccacgccat cagcagaccg aaaacgccgt
    19861 taaaaatcga tgccacaaac gccgacagca gcgtcacttt ataggctgcg accacctgcg
    19921 ggttggtgat cacctcccag tactgcgccc agctcatctg ggccagttgc atcaccagcg
    19981 cggagagcgg cagcagcaaa atcaggcaca caaacagcag actggtgccg aggcttaagg
    20041 taaagcccgg cagcacgcgt cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa
    20101 cagcttgtct aactcgccgc cgctggtgaa gtgggttttc atcacttccg gccaggagcc
    20161 aaatttatct tccacgcgga acagctcggt ctgcgggaat ttatctttca gtttgcccat
    20221 gacttccggg ttatttacgc ggtagtaata gtcggtgatg atggtttgcg cctgcgggct
    20281 gtagagccag ttcaggtagg cttttgccgc tttttccgtg ccgttggcct gtacgttttt
    20341 atccacccac gccaccggga attccgccag aatgttggtt ttcggaatca ccacttcaaa
    20401 gccctgcgct tcatactgtt tacggatgtt gttcacttcc gactcgaagc tgatcagcac
    20461 atcgcccagg ccgcgctcgg caaaagtggt ggtcgcgcca cgaccgccag tatcgaacac
    20521 ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt ttggctttat caccaccgtc
    20581 ggctttgtcc gctgcgcccc atgccgcaag gtaggtataa cgcgcgttac ccgacgtttt
    20641 cgggttcggg aaaatcagct tcacgtcaga gcgtaccagg tcgttccagt cgtggatatt
    20701 cttcgggttg cccttacgca ccaggaagcc catggtggag tagaacggcg agctattatt
    20761 cggcaggcgc gactgccagt ccgctgggat cagcttgcct ttatcgtgca ggatttgtac
    20821 gtcggtcacc tggttataag tgacaacgtc ggcttttaaa ccctgcaaaa ttgccagcgc
    20881 ctgttttgat gatccggcat gagattgttt aatcgtcagt ttgtcgccac cgttatcttt
    20941 tgcccattgt tgctcaaacg gcgggttcag ggcggcaaac agctcgcggg agacgtcata
    21001 agaactgttc agcagttccg ttgcctgtac atggcccgcc agtagcagag aagcaaccag
    21061 cacgagtgag ttctttttca gtaagttaac ggccattgcg cacccttata aatttaatga
    21121 ctttctaata gccatcatat ttataacgga cgtgaaagga gtaacggttt tatataccgt
    21181 ttggtgattt ggaagttgaa aagggaataa gacgaaatcc tccccacaaa tgcagggagg
    21241 aaacagaggt gaatcagatg ccgacgctaa ccgtctccgg cagtgtgctg ccgccatcaa
    21301 tcacattctg tgtaccggtt aaatagctgg attcatccga tgcgaggaag gccgccagtt
    21361 cgccgacttc cagcggatcg gcgaggcgac gcatcgggat tgctttcgcc atttcagtca
    21421 gcaccgattc tggatcttcc gggttcgact ggcgggcaat gctttccgcc attggcgtgc
    21481 gaacgtatcc agggcagatg gcgttaacgc gaataccaga ctgcgcgtac tccaccgcca
    21541 gcgatttagt caggccaaca atcgccgctt tcgttaaggc gtacgccgtt tcgcccgggt
    21601 ctgccaccat atcgccagtg actgaagaca tcatcacaat ccggccatct ttgcgggcaa
    21661 tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc ttttatattg atatcaatat
    21721 ggaaatcgcg atcttcatcg ctcatatcaa ggaaactgcc cagacgacaa acgcctgcgt
    21781 tattcaccag aatatcaatg cgcccttctt tttccttagc gcgcttaata gctgcggcta
    21841 ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca gcgatgacca cggccacaca
    21901 gttcgtccgc cagcttttcg atctcagggg agatatccag caagattagg tttgcgccat
    21961 gacgcgcaaa agttctggca attccttcgc caattccctg caatgcgccc gtaatcagtg
    22021 ctgtcttgcc cgtgagttta cccattttaa tgctctcctt gttaatacag agcgttacac
    22081 tccattccct ctgaaaatac aggataaaca aaatattgct gcggatcagc tcactaattc
    22141 aggaaaatct caatttagtc gctgctttat gcagcacctc aagcgttacg tcagcgcgca
    22201 atcattttca gtgacatcac atgagtaata ttcggactta atgattcctt tgtgtctatc
    22261 aaatcgggat atatgatccc atttatctgc aattatttga tcgcaaatat taaggtcacc
    22321 accgatgcaa cttgaaaaga tgattaccga aggctcgaac gccgcctcgg ctgaaattga
    22381 ccgcgtatca acgctggaaa tgtgccggat tatcaacgat gaagataaaa ccgtaccact
    22441 cgccgttgag cgcgtactgc cggatatcgc cgcggcgatc gatgttatcc acacccaggt
    22501 aagcggcggc ggccgtctga tttatctcgg tgcgggaaca tccggtcgtc tggggattct
    22561 ggatgccagc gaatgtccgc cgacctacgg cgtgaaaccg ggcctggtag ttggtttgat
    22621 tgctggcggc gaatatgcca ttcagcacgc ggtggaaggc gcagaagata gccgggaagg
    22681 tggtgttaat gatctgaaaa atattaattt aaccgcgcag gatgtggtgg ttggtattgc
    22741 ggccagcgga cgcacgcctt atgtgattgc cgggctggaa tacgcgcgcc aactcggctg
    22801 ccgtacagtg ggcatttcct gtaatccggg gagtgccgtt tcatccaccg cagagtttgc
    22861 tattacgccg gtagttggtg ccgaagttgt taccggttct tcgcgaatga aagcaggcac
    22921 ggcgcagaaa ctggtgctca acatgctctc caccgggctg atgattaaat ccggcaaagt
    22981 gttcggcaac ctgatggtcg atgtggtcgc caccaacgaa aaactgcatg tgcgccaggt
    23041 caatatcgtc aaaaacgcta ccggatgtaa tgccgaacaa gcggaagcgg cgttaattgc
    23101 ttgcgagcgc aactgtaaaa cggccattgt gatggtactg aaaaatctcg atgccgatga
    23161 agctaaaaaa tgcctggatc aacacggcgg ttttattcgc aaggctctgg aaaaggaata
    23221 acccatggct aaagagatca gcagtgaact tctgaacacc attcttaccc gggtcggcgg
    23281 accgggaaat atcgccagtt gtggtaactg tatgacgcgc ctgcgtctgg gtgtacatga
    23341 cagttcattg gttgatcccg atatcaaaac gctggaaggc gtgaagggcg tcattttgac
    23401 cagcgatcag gtgcaggtgg tttttgggcc aggcaaagcg catcgtgccg caaaagccat
    23461 gagcgagctg ctgggcgagg caccggtaca ggatgccgca gaaatcgccg cccagaataa
    23521 acgccagtta aaagccagac aaacttccgg cgtgcaacaa tttctcgcca aattcgccac
    23581 catcttcacg ccgctgatcc ccggttttat tgccgccggt ctgctgctgg ggatagcgac
    23641 gttaattgcc acggtgatgc acgttacggc agatgctcag ggcacgctac ccgatgcgct
    23701 gaattttatg aaggtgttca gcaaaggcct gttcaccttc ctggtaattc tggtgggcta
    23761 taacgcggcc caggcatttg gtggcacggg cgtaaatggc gcaattatcg ctgcgctctt
    23821 tttgcttggt tataacccaa ctgcaaccac gggttactac gccggatttc acgatttctt
    23881 tggtctgccc atcgatccgc gcggcaatat tatcggcgtg ttgatcgccg cgtgggcctg
    23941 cgcccgcatc gaaggcatgg tgcgccgctt tatgcccgac gatctcgaca tgctgctgac
    24001 ctcgttaatc accctgctga tcaccgctac gcttgcgtac ctgatcatta tgccgctggg
    24061 cggctggctg tttgaaggca tgtcgtggct gtttatgcac ctgaacagta atccgctcgg
    24121 ttgtgcggtt ttagccgggc tgttcctgat cgccgtggta tttggcgtgc atcagggctt
    24181 tattcctgtt tatctggcgt taatggacag ccagggattc aacagcttat ttcccatcct
    24241 ttcaatggca ggcgcaggcc aggtgggcgc ggcgctggcg ctctactggc gggcgcaacc
    24301 gcacagtgca ttacgcagtc aggtccgcgg ggcgattatt cccggtctgc ttggcgttgg
    24361 cgaaccgctg atttacggtg tcaccctgcc ccgcatgaaa ccgtttgtta ccgcctgttt
    24421 aggcggcgcg gcgggcggtt tgtttatcgg cctgatagcc tggtggggtc tgccgatggg
    24481 cttaaacagc gccttcgggc cgtctggtct ggtggcgctg ccgctgatga cttccgagca
    24541 aggcatcctt ccggcaatgg cggtttacgc tggcggtatt ctggtggcat gggttagtgg
    24601 gtttattttc accacactct ttggctgccg taacgtcaat ctggactgat tatgaaacgg
    24661 acaatgctct atctttctct gctggctatt agctgtagcg tcagcgccgc aaaataccct
    24721 gttctgacag aaagtagttg atcaagtgta tgaggcgtta aagcagtaat aaaaaggcca
    24781 gtcagttgtg ttactggctg gcctttaatc aatgaatcag aaacgcttac agcgccatca
    24841 gcttgtccag cgacggtgcg aagtaatagc cgccagtgac cggtttggtg aaacgcaaca
    24901 tcgcatcacg cttaccatca gtatcgccaa acatgctcag caattgctgc tcaatgttat
    24961 gcagacgcgc gcagtaggcg cagaagtaca gaccgtgagt gccactggcg gtgccgtacg
    25021 gcaggctctg acgaacaatc ttcagccctt tgccatcttc tttcagatca acgcgggtca
    25081 ggtgagaggt ttccggacgt tcgtcgccgt caatctcttc gttggcctct ttggtgcgcc
    25141 cgatcatcat ctcttgatcg tgaacgctca tccgattgag ctgcttcagg ttgtgctccc
    25201 aacgctgcac aaacacatag ctgccgcccg catccacgcc gtctttgata acagccactt
    25261 cgcggcgcgt ctcttcaccc gccgggtttt ctgtaccgtc aacaaagccg ctcaggtcac
    25321 gctcttcaac ccaacggaag ccgtggatct cttctttcac ttcaatgcag tcaccaaagg
    25381 cttccatcgc cgcctgagcg acagagaagt tcacgtcgtg acgcagagaa agaatgtgga
    25441 tcaacacatc gaactgggtc gtcggtgcaa gacctttacc gtagcccgga aaatctttca
    25501 gctcttcggc cccaacgccg ccgctcagag tgcgccaggt attgttacca aaggcaacca
    25561 ccgcaccaag atgcgcgtcc gggaatttcg cttcaaaagt tgccagtttg tcggcaaatg
    25621 ttttactggc cgcacgcagg gcgtcaactt cccctttcac gttggcttcg atccaaatcg
    25681 ccgcgcggca atgttctggc aaaatgccac tctgaacctg agacattgtt cctcctgaaa
    25741 ataataatgc cacgcaatcc gtggcattgt tgggcgctat tttacgtgtt ttttcgcggc
    25801 ggcggtttgt tcagacgcaa attaacgccg ccagataatt ttactgactt tccagttttt
    25861 cagggtatcg tccgaaggca ttaacccttc aggaccactc cattccccgc tgaactggta
    25921 gctaatatgt tgactgcctt cggctttaca ttcgacggca cgattatcat cactgtcggc
    25981 cttttggcaa ttgccaaatg ctttgctgta aaagtcacta aacggtgtac cgattttaac
    26041 accggtgtca gcaggaatat cgctatccag cacatcaatg cggctgatcg tgccttgatc
    26101 gccgttaatc accatcgcca cgttgtcgcc tttcatcact tcaaaaaagc gcaccacgtt
    26161 gccgttcgcg gttttcattc cgctgcgcag gcgataatcg ccatcaagcg catcagcaat
    26221 ggcttgttct tgcagtggtg tggacgccgt taattcaccc acgccctgct cgctcacttt
    26281 ggtggatgat ccaaaccagt tccacgggtt agcggccgac cagttaactg acgacatcgt
    26341 ggaacagccg gtcagcatca acggcatagc gcataacatt aaacgcagcg atttcatgtc
    26401 acttcctttg gttattcaat aacgttgatt ggagtgcaaa ttcaccaaaa agtgccgtta
    26461 ttctttttct tcattaaaac aagcgcgtaa acgtcgattg gtcagcagcc agattaacgc
    26521 cacgatatcc gccactacca gcgccagacc gataccgcta acggattcac cgttcagcca
    26581 cagccacggt tgccaacaaa gtaaaaccac ttgcgccaac agcaacagaa aatagagcac
    26641 atgccagata cggggaaatg aagcccgccg accactcagc aaaaatgcca gcaccgccgg
    26701 aataccggga atcaacccca gccagaaatt atcgtgatcg ggataaaaca gatttagcag
    26761 cgcagtaccc tgctcgcgcg acgctccggc aatgacaaac agcacccagg ttcgcgcctg
    26821 aagcaatagc acaagccaga agagcaaggg taaacgcagg cgaccgtgcg catcataatg
    26881 gacaggatga aactcagtac tcttcatctt caatcaaacg cttacccaga ctcagcacgt
    26941 cggcgtgttc atatcccagg cgttcataca ttccaagcac catgtcgtta tcttccggca
    27001 cgttgatctg aattttcggg cagccgcgag caatcagctt tttctccaac cgattaagca
    27061 acgcattagc aatcccacgc ccacgaaact ctggatgcac gccaagataa taagcagacc
    27121 cgcgatgccc gtcataaccg cccatcaccg ttccgaccac ttcaccgttt acctcagcga
    27181 ccagaaacaa actgacgtca tggttcatct tacgctcgat gtccatttcc ggatcgttcc
    27241 acggacgcag caagtcgcaa cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc
    27301 gaaatacgcg tatctccatg gtattcgtta cctttttgcg ggttaaaagg ctgattatgg
    27361 cgtgaacggt cgaattagcc aatatctgac gaaaatcggt tgaaaaagtg gcataatggg
    27421 gagttgtcaa ctattgaaat gaaaagtaaa acaattctca acagcaaacc gtcgtaacgg
    27481 attacgcgat acgatataac atctggaact ttattattac aactcaggcc gtatgagcac
    27541 ttttaaacca ctaaaaacac tcacttcgcg ccgccaggtg ctgaaagccg gtttggctgc
    27601 cctgacgttg tcaggaatgt cgcaagccat cgccaaagaa gaaccgttaa aaaccagtaa
    27661 cggacacagc aagccgaaag caaaaaaaag cggcggcaga cgtgtcgttg ttctcgatcc
    27721 aggccacggc gggattgata ccggagcgat cggacgcaac ggttcgaaag aaaaacatgt
    27781 ggtgctggca attgccaaga atgtccgttc catattgcgt aatcatggca ttgatgcgcg
    27841 tttaacgcgt tctggcgata cgtttatccc actgtacgat cgcgttgaaa tcgcccataa
    27901 acacggcgca gacctgttta tgtcaattca cgccgacggt tttaccaacc cgaaagctgc
    27961 cggtgcttcg gtatttgccc tctctaaccg tggggcaagt agcgcaatgg cgaaatacct
    28021 gtctgaacgc gaaaaccgcg ccgatgaagt tgccggtaaa aaagcgactg acaaggatca
    28081 cctattgcag caagtgctgt ttgatctggt gcaaaccgat actattaaaa acagcctgac
    28141 gctcggctcg catattctga aaaagattaa gccggtgcat aaactgcaca gccgcaatac
    28201 cgaacaagcc gcgtttgtgg tgttgaaatc gccgtcggtt ccgtcggtgc tggtggaaac
    28261 ctcgtttatc accaacccgg aagaagagcg gctgttaggc actgcagcgt ttcgccagaa
    28321 aatcgccaca gcgattgccg aaggcgtgat cagttatttc cactggtttg acaaccagaa
    28381 agcacactcg agaaagcggt aagtgatgaa acccgacgca caccaggtta aacagtttct
    28441 gctcaacctt caggatacga tttgtcagca gttgagcgcc gtcgatggcg cagaatttgt
    28501 cgaagatagc tggcagcgcg aagctggcgg cggcgggcgt agtcgggttt tgcgtaatgg
    28561 tggtgttttc gaacaggcag gcgtcaactt ttcacatgtc cacggtgagg cgatgcctgc
    28621 ttccgccacc gctcatcgtc cggaacttgc cgggcgcagt ttcgaggcga tgggcgtttc
    28681 actggtagtg catccgcata atccgtatgt ccccaccagc cacgcgaatg tgcgcttttt
    28741 tattgccgaa aagccgggtg cagagcccgt ctggtggttt ggcggcggct tcgatttaac
    28801 ccctttctat ggttttgaag aagacgccat tcactggcac cgcaccgccc gtgacctgtg
    28861 cctgccattt ggcgaagacg tttatccgcg ttacaaaaag tggtgcgacg aatacttcta
    28921 cctcaaacat cgtaacgaac agcgcggcat tggcgggctg ttctttgatg atctgaacac
    28981 gccagatttc gaccactgtt ttgcctttat gcaggcggta ggcaaaggct acaccgacgc
    29041 ttatttacca attgtagagc gacgaaaagc gatggcctac ggcgagcgcg agcgcaattt
    29101 tcagctctac cgtcgcggtc gttatgtcga gttcaatctg gtctgggatc gcggcacgct
    29161 gtttggcctg caaactggcg ggcgcaccga gtcgatcctg atgtcaatgc cgccactggt
    29221 acgctgggag tatgattatc agccaaaaga tggcagcccg gaagcggctt taagtgagtt
    29281 tattaaggtc agggattggg tgtaactccc tcacccccac tctcgcatcc gctgatgcag
    29341 cgtcagtgac ggcttctcgg caaacagttg ctgataatcc gtggcaaatt gcccaagatg
    29401 ccagaatccc cactgcatgg cggcgtcttt taccgttgtg ctttgtgacc acggacttat
    29461 gagttcgcgg cgcacggcgt tcaggcgaat acgtttcagc cacgcgttcg gaccaatgcc
    29521 caaaatggcg tgaaacgcgt tttgtagcgt gcggcggctg acatgcagtt gattacacaa
    29581 gtccagcacc gtcaccggtt cggacatgtt ttccagcaca tattcacggg cgcgggaaag
    29641 caatcgacgg taactctgat gactgatgct ttccgccgtc accattggct gcgcttcttc
    29701 cagcatcgcc cccatcgcca ttagcaaatt atcccccagc acttttcgca ctgcaggctg
    29761 atggagattt tccgggtttt cgcaaaacgt cgccagcgcc tgttggacaa agccccacag
    29821 agcggcttta tgctgctctt tcacttccag cgccgactgg ctacgcagca tatgtaatac
    29881 ccgatccgga ttatgcaaaa agttagcctg acgagtgatg acatcttcag aaagcactac
    29941 gccaaggatc gtgtaatcgt ccggcgtact taattcaaac tcggttccac cagggcgcgt
    30001 cgcaatttct gcacttccca gacattgcga accgataaat ccctgctcac cgcgcgtcgc
    30061 cggaatgcca aaccagaacg agttcggcca gaccaggcac gactgacgca gcgccagacc
    30121 agtgtattca cgaaaaacct gaatatcatc gagtagaatt tccgtaaatt caccatgaaa
    30181 cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg gtaattgtca aagcatgttc
    30241 atagacgtcc gttgtctgtc gttgatgaac attatccact tcgaccttcg gcgtgagctt
    30301 caggttttcg ggtaagggtt catgataaag atggtgcaaa ttggctgtac gggtcttttt
    30361 catgatgtta atgccgggtg ttgtaggaca cccggcacct ccgacaggtt aatggggctt
    30421 gagacggtaa cgactactgc gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc
    30481 atttctggct ttttccagcg gccagccaaa gtgggcagcc acttctcctg ccgtcattcc
    30541 ctgacgtact gatgttaaca gcgccaacaa ctcgtcagca gattccgcga cgataactgg
    30601 cgcgtcgggt tcctttacgg gttgcttcgg ctggcggtta aagccagtaa ccagccactg
    30661 agtgtcatcg tctggccgac cgatctcctt acggctgatg acacgcccgg tacgcatcgc
    30721 ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt ccaccaccag
    30781 cgttaggcga ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag
    30841 catggcatca gctgcatcta tcgcggcgac catgccgtcc acttccagca atcccagtgc
    30901 attgatcatc ggcaacctcc gttacgcacg ctggattgga ttacgcgcga tttccagcac
    30961 tgcatcggta aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc
    31021 tgccgagtag ttggtttcag acggtggcgg gacataggtc accagctgca cgtcggcaga
    31081 tttcaacgct gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat
    31141 cggatcgcca agcgtgatcc cggcggttga tgagagataa gaaccggtac gcgaaactac
    31201 atgtgctagg aaggctgtgt tttctgcgtc attagcccac tggaaagcag cgccattttc
    31261 aatatttgca accatcgcat ccagaccagc acgcacttcc gccgggtttg gtccaccaag
    31321 cataatcagc acctcaccgg cagtcggtga cgggccgtgt gcagcgcccg catacagcga
    31381 gcgaccatat accacttcga ccatcgcctg cttggtcgct tcgtcagccg caatgtaggt
    31441 gacgtcatct gaatcagcag aaatcagccc gaggctacga atatgcggcg gcaatttcag
    31501 ttcacgtgca aattcggcgt taacggaggc aatcacccgc atggcggtta ccgacggtcg
    31561 aatcaaatct aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt
    31621 ctgctccagc atacgtttcg ccaaatccac aatgacggct gcggcttcaa ccggcggcgt
    31681 gccgccctgg tgaatattag agatacaggt gcgatcggcc tcaacggtgg tcgccatacg
    31741 cggcgagtaa acggcgtagc aggagaggct ttctgactgc cccaaccccg gacgttcgcc
    31801 caccagcagg atcactactt tcgcgcctag gatctcaccg atctgatctt caatcttcac
    31861 gcgaccataa cgtacaaaga atggcgtacc gactttcagc ccggcttgct tcaggcccgc
    31921 catcagcggc ggcaggatct cttcgtagtt cacggtgatc gcatcagttg acaggccatc
    31981 agaaataacg acctgcacgt ccggattagc aacacactgt gctttcagcg cttcaacagc
    32041 ttctgcacac aggcggcggc ccatatccgg acgagtcagg tagagatttt tgtcgctgat
    32101 ttcggagcgg acttccagca agccctgcgc tttcacccac tcttccggta cttctttcag
    32161 aacggtatct ttcgaacgag agtgatcggc caggaaacgc agcaacgcct gggtgcgcgg
    32221 acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg ctgcgacgca gttctgttaa
    32281 tacgtccgcg cgatgtgggt tttccacacc aatccacgct tttgcttccg cagaacctaa
    32341 atccagcgcg cagctttccg aggtcaccgg tgccgcacag ttggtggtgg cgcactttgc
    32401 ttctgacggc gcgggggccg tttgtcccat tgacgccatc acactgcgta caatttcttc
    32461 aatctgtttt tgatccatga tgtgttatct ccgcgtcatc agaagaacag tgacggatcg
    32521 cccgcccgtt tggtcaggcg accgtttgcc ataatgccca tgctttccag ccagcgttca
    32581 aactccggtg acggacgcag attcagtaac tgacgcacag tggcagtgtc gtggaatgcg
    32641 gtggtctgat agttgagcat gatgtcatcg cccagcggca tccccatgat gtagttacag
    32701 cctgcggtgg cgagcaggat catcaggttt tcgttgaggt tctggtcagc gtcagcgtgg
    32761 ttggtgtagc agcagtcaca gcccatagag atgccgctca gcttgcccat aaagtgatct
    32821 tctaagcctg cgcggataat ctggcggtcg ttgtagagat actccggccc aataaaaccg
    32881 accacggtgt tgacgataaa cggatcgtaa tgacgcgcca gcccatagtt acgcgcttcc
    32941 atcgtcacct ggtctgcgcc gaagttagcg ccagcggaca gcgccgagcc ttgtccggtt
    33001 tcgaagtaga gacagttttc cccggcgata cggttgaact ccgcgcccac tgcgcgtgct
    33061 tcatcgagca tcgccagctc tacgccaaac tctttcagcc ctttttcgct gccgcagata
    33121 ctctggaaaa tcagcccgcc tggtgcgcca cgacggatcg cttcgatctg ggtggtgacg
    33181 tgcgccagta cgcagccctg agttgggatg ttgaatttgt cgatcacgcc ataaatggta
    33241 tccaacacgc ggcttaagtt ttccacgtcg tccgtcaccg ggttgacgcc gatcaccgca
    33301 tcgcccaccc cgaaggaaag cccttcgtag atttgcgcgg cgatactttg cacgtcgtca
    33361 cgggtatcgt tcggctgcaa acgggcgcta aaggtgcccg gaataccgat ggtggtattg
    33421 gcctttttga tcaccggcat tttcttcgcg ccgtagatca ggtccgcgtt ggagcaaatc
    33481 ttcgctaccg ccgccaccac ttccgaggtc agccctttgc gggtaaaggc aatgtcgtcc
    33541 acgctggttt catcgctcag cacatactca cgcagttcgc tgatgctcca gtttttaatc
    33601 tggttgtagg ccgtttcgtt aacgtcgtcc tgaatcagcc gcgtcacgca gtcatcttca
    33661 taggcaatca ccggattatt gcggatgtct gctacggtca tttccgacaa cacctgcttt
    33721 gccgccacgc gctcctgtga gcttgccgca gcaacgcccg ccagcacatc ccccgaacgc
    33781 agttcgttgg ctttagccag cacctctttt acatccttaa actgatatac attgccgaac
    33841 aatgtggtct ttagtttcat aagtcgttcc ctcaggaagg aaatgcgagt gatttcaccg
    33901 tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc
    33961 ggacaatcac ttcgtcaatg actgccaacg ggagttgttg tagctgtggg cgcaacaaca
    34021 tgcccagagc tttaccaaag tcctgcccgg ccaccaccag caggggatgt ggattcggaa
    34081 aacgcgcgac gaaatcgacc agcgcgttga tgaccgtcag taccgcggcg taacgcacag
    34141 gcagcgaggc gggaagcgcc agcacgtacg cgtcagtttt ggggcaaaga tccagttgaa
    34201 tcagcgcctg ttgccaggca ctgaccagat ccgtttcatc aatcgggatc gccaccggca
    34261 aattgcgcag cggcagttgt acgccctcca gccagattgt gctgccagag agcgaaaggg
    34321 tatgtgcacc cgcgccaatc accgtggcgc gcacggtttg cgccggaaac tgcacgttca
    34381 tctcacgcag acgcggatgg tcatgcagcg ccgttgccag aagcgggccg atatcggcaa
    34441 aacagaacgg gtcggcgggc tggtggcgat aacattcgcc cacgccgcca gaaagcgtaa
    34501 tgatttcggg cgtaacacct gcgggcagca aaccggtttg catcaatgcc tgcgcgagcg
    34561 gcgagagcgt tccgtcaatc acttcgacaa tcagctctgc catccggcgg gtaacctgca
    34621 ccagctgccc gccagtcagc gaacgggcgt cagtgcctgg gccgaagcac tcatccacaa
    34681 tcatctgccc cggtttatga gcgtaaacca cgcgcccctg gctgtcggtt tccagcaggc
    34741 gaccgccgac gttgaggcag gcagtcccgc tgatttttcc ggcatcgaac agggcgtagt
    34801 tcgcggtgcc accgccgata tcgatattca ggacccgaca cagccgttgt tcagaaaggg
    34861 tttgcgcccc ggcaccgtga ccggcgatca cggattcgag atgcggtcca gcgctggcga
    34921 cgacaaaatc gcccagcgac cgggagaggg ccatcaccgc cgggcgagcg ttgcgggttt
    34981 ttgcgctttc accggtgata atgatggcac cagaatcaac gctttccggc gcaatgcccg
    35041 cagcctgata ttgcttgagg attaaggttt tcagttccgc ttcttttaaa ccaccctgtt
    35101 tatcgacagg ggtaaagaac accgggcttt gccagctaat ttcgcgtttg atgaattcgt
    35161 agcgcggcac ctgcgacacc gccgcacggt taaccagctc caggtgggag aaaattacct
    35221 gggtggtggt ggtgccgata tcgataccga cgctcagtag ctggcgagtg ttcacgattg
    35281 cgcctccgct tcggttttag ccgcgctcgc gtcttcttta ggtaccagca tcatcgccac
    35341 gccaatcgcc gtgacgccgc cgatcaattt gccgacaatc atcgggaaga tcatggcgtt
    35401 catgttggca gccgcgaagc ctaaatggtc gcccagcgcg aaagcagcgg aaacggcgaa
    35461 ggcacagttg atgactttgc cgcgggtatc catctgcttc atcatgccaa acatcgggat
    35521 gttgttggca agcgttgcca ccatgccggc tgccgcgatg ttgttcatat tcagcacctt
    35581 accgacgctc atcagcggtt tttcaaacca gcgagtcagc agcagcacca tcggatacgc
    35641 ccctaacaga acgcaggaga tcgagccgat aacttcaatg gcgcgcatca cttcaccggg
    35701 tttatcgcca ggggccataa agataggatc aaggcccggg atcagttccc agccaaggag
    35761 gaatttcact accgcagcgg caagaccgag agtgatcaat gcaacgagga atttggcgaa
    35821 gatctggaag ccgttgatca ttttttccgg gatgaatttc agccccagcg ccaccagcac
    35881 cgcaacgata agtaccggga tcatgttcat caggatcagc gcaaaggtga attcaacagg
    35941 ctggccgttg atctgcacac cggagtacat cgctaccaga ccaccagcga tacagccaat
    36001 cggaatggtc actatgcccg ccagctcttt ggcgaggaag aagcccccca tatcgcacgc
    36061 cagcagtgtt ccggcgaaca ttgacgggtt tgcgccgagc atttcgtaaa ccgggataat
    36121 caccggtccg agaacgtggg ccagtaccgg tgccagtgcg gtcataccga ccatagccag
    36181 gcccagagcg cccattgcca taaagccttc ttcgaactga ccgccggatc cttcgatact
    36241 tttaccgaac ttaccgagga aacgagcaga accgccgaac tgcgacagga tcctgtctac
    36301 ggcagctatc agcataaaga acatcatgat gtacatgatg atttcgttaa ttcccatcgc
    36361 ctttactccc tgttagttgt tatttattgg cggatgcggc gtgaacgcct tatccgccct
    36421 acatgtgcaa tcccgaaggc cagataagac gcggcaagcg tcgcatccgg caattgcacc
    36481 gcgccactgg cggatgcggc gtgaacgcct tatccgccct acatgtgcaa tcccgtaggc
    36541 cggataagac gcggcaagcg tcgcatccgg catttattgc gccgctgcgt acaggccgac
    36601 aatctgctcc aggctggcgg tacgcgggtt gctgcgcaga caaatatctt cctgcgcggc
    36661 ctgcgcccat gcaccgtaat gcgcagatgt cgcaccaaca tcgcccagtc gtttaccaat
    36721 cccaacttca gcaatcagct cacttaccgc gttaatagcg tcacgatcgt cggatttttt
    36781 agttcgcagc gcccgaccaa tctgactaaa ccgatcacga caaaccatcc ggttaaattc
    36841 catcaccgtt ggcaacaaca tggcgttcgc cagaccgtgc ggaatatgca gcgccgcccc
    36901 tggctgatgc gccatcgcgt ggcataaccc aagcccggcg ctggaaaacg ccattcccgc
    36961 catacatgaa gccagcaaca tgctctcgcg cgcggcaagg tcatggccgt agcccaccgc
    37021 tttcggcagt gatttgccaa tcatcgcaat ggcaccaatc gccaggctgt cggtaaacgg
    37081 tgtagcgttc agcgcgctgt acgcttcaat ggcatgggtt aacgcatcaa tgccagtcat
    37141 cgccgtgaca tgcgacggca cgccttcggt cagcacagcg tcgagaatcg ccacatccgg
    37201 catcagcgag gcgtgggcta acacctgttt gcgcccgctt accgcatcga taatcaccgt
    37261 cacgttagtc gtttcagagc cggttccggc ggtcgttgga atggcaatca acggcaggcg
    37321 cggttgcaga acgctggttt ctgacatttc tgccagcgtg ctatccgggt tcgtcaccag
    37381 caacgccacg gctttcgccg catccagcac cgagccgccg ccaaacgcga tcaccccgtc
    37441 acaacctgat tcacgcaact gcgccacggc tgcacacacg tcggtgatgc acggttcgcc
    37501 caccggacat ggccagagcg tcatggcgat acctttaacg gccaggctac gtgtcagccc
    37561 ggcggtcatc cctgcctgat gcaaaaagct gtctgccatc acgaacagat gtttcaaccc
    37621 acgcgtttgc gcttgctgcc cgcaactgct caccgcgccc ggaccgcaaa gcgtcaccgg
    37681 tggaatgcta aatgttttta cccgttgcag attcagggta tcgaacgcct gaaaaagcgc
    37741 cgtttgcaat tcactttgca tagatcccct ccgctttctc tcgcccacta ctggcgattg
    37801 ccagcggggt cataaacaaa ctgtgctgcg gtaaatgcac ctgtaacgcc ggaaattgtt
    37861 tgcaaaacag cgcagctacg cccggttgca tacaggaacc gcccgccagc cataaatccg
    37921 taatgccctg cccttcaata tgccgggcca caatgtccgc cattttttcg tacaccggtt
    37981 tcaccgcggg ccaaatctcg tcaccgtgac cgcgtttgta ctgctctgcc tcttccagcg
    38041 aaatacggcg atttccggcg agagtcagag agatgtgatg tccgccggtg gcttcatccg
    38101 ccgagtacgt cactttgccc tttttcacga tggcaatgcc ggtagtgccg ccgccgatat
    38161 ccactacacc ggcgttgtcc agttgcagca gatccgcaac tgcggttggc tcatccagca
    38221 catggctcac ttccagtccg gcagactcca gcacgttgat ggaaatacgc gggtccgtac
    38281 ctggaggaaa agaggtcgcc gcatggctaa aacggcgacc gaattgctgc tcgagggtgt
    38341 cgagatggcg tcgaacaatg gtgaccgcac cgaagaaatc ccagacgatg ccgtcgcgga
    38401 ccacatcagc ccagtcgagg cataccgcca ccggctgacc gtcgcggtcg acaaccatcg
    38461 acaccacatc gcaagtgccc agatccaccc ccagccacag cggagattcc gtcgcggcag
    38521 gcgtctggtt acacagcgtt gccgccgttt gcagacgtgg ggtgagccat tgttcatcgt
    38581 gcgccatcgg ttactcctta aacaatgcga aacgcatcga ctaatacaca gcgacgcaga
    38641 cggacaaacg tccgcgcgct ggttacccct tcaccggttg gcgtggtgat ggtcatcgtg
    38701 gtccagcctt ccccgcccag ccccagcccg gcaatacacg gtccgttctt aacgaaaatg
    38761 ctggtatcaa tggcgttcgc catctggttc atgttttcga tattgcgcga gtgcattgcc
    38821 gccgtgtggt ggcaaccgcc ttccagtttc accgccagcg caatggcatc cgccacgttg
    38881 gcgacgcgca cgacgggcag caccggcatc atcagttcgg tcacggcaaa cggatgttct
    38941 gcggtggttt ctacaaacag cagttgcgtt tgttccggaa ctttaaggcc gattgccgcc
    39001 gcgattttcg ccgcatcgcg accaacccag tcacggctga cggtgccttt tccgcgctcg
    39061 tcgatatttt tcagcaacac cggttgcagt tgctgcgcct gctctgcggt cagtttcacc
    39121 gcatgctggc cttccatcag acgcatcagt tcatcggcta cgctatcaac aacaatcagt
    39181 accttttcgt cggcacaaat gatgttgtta tcgaaagaag cgcctttgac gatggactga
    39241 gcagcacggg cgaggtcggc ggtttcatcc accactaccg gcgggttgcc agcgcctgcg
    39301 gcaatcaggc gtttattggt gtgtttgcgc gccgcttcca ctaccgcttc gccgccggtc
    39361 accaccagca ggccgatacc cggaaacttg aacaagcgtt gcgcggtttc gatatccgga
    39421 tttgctacgg taaccagtaa gttttccggc ccgcctgcgg caacaatcgc ctggttgagc
    39481 agcgtgatcg cccgctggga gacttttttc gccgccggat gcggggcaaa aatgacgctg
    39541 ttgcccgcgg caatcaggct gatggcgttg ttaattacgg ttgccgccgg gttagtggaa
    39601 ggcgtcaccg aagccaccac gccccagggt gcgttttcaa ttagggtcag gccgttgtcg
    39661 ccagtcagca cttgcggaga gaggcactca acgcctggtg tgccgcgcgc ctgagcgacg
    39721 tttttggcaa atttatcttc aacgcgcccc atgccggttt cactgacggc aagttccgct
    39781 aaatctctgg cgtgtttttc gcctgcttca cgaatggcag caatggctaa ctggcgcatt
    39841 gccacgcttt ttaacccttg ctgggcgact ttggctgccg caacggcgtc atccagggac
    39901 gcgaaaacgc ccatctcatg aacggcggcg ggcggcgtgt cactgctttg cattttcagc
    39961 agtaccgctt tcaccacctg ttcaatatcc tgttgattca tgatgttctg ccttatttgt
    40021 ggaaaatgac ctgaccgcca gacaccacct catcgacgat gccaatcacg cacagatcga
    40081 ccggtgacgt ttcgctttta tgcgcctggc gggcggaact gccactcacc agtaacaccc
    40141 actccccggt tcccgcgcca atattgtcga tggcgacggc gcattgcccg tcaggattac
    40201 cttgtggatc aatcatttcc accatcagca atttgtcatg cgccagtccg tgatggcgta
    40261 cggtacaaac aatttgtcca gtgacgactg ccagtttcat acccgcctcc gtggcgtatt
    40321 tcaggtaaaa gctcccccta ccctccgcgg aaggtaaaat gaaaaaggag agagcgtgac
    40381 gcccgaatcg acgtcacaca gggtgattac aggttgctgc tatcgccttt caggccgatc
    40441 gggaagactt cttccaggtc gccgtgcgga cgcgggataa cgtgtacaga taccagctcg
    40501 ccgatacgct gtgccgctgc ggcacctgca tcggtcgcgg ctttacaggc tgcaacatca
    40561 ccacgaacca tggcagtaca caggccgccg ccaatctgct taacaccaac cagcttgacg
    40621 cgcgcagctt taaccatcgc gtcagaagcc tcaatcagtg caaccaggcc ccgagtttcg
    40681 atcattccta atgcttccat tgtgctttcc tctttatcag ggtccagaac gggaccgttc
    40741 attcaaccag tgtttgtaaa ctgctttcgc ggttcacttc tgtctgacgc ggcacagctg
    40801 ccaccagcgc cagttcgata atttcctgca cgctacaacc acgagagaga tcgtgcatcg
    40861 gcgcggcaag tccttgtatc aatggcccga cggcacgata tccgccgagt cgttgtgcga
    40921 ttttgtagcc aatatttcct gcctccagcg acggaaaaac catcacatta gctttgccct
    40981 gtaacgggct ggcaggcgct ttttgcgccg ccacttccgg cacgaaggcg gcgtcaaact
    41041 gcaactcgcc atcgacaacc agctttggtg cgcgctcacg gacgatttct gtcgcctgct
    41101 ggacgttggc aacgcagggg tgacgggcac taccgttact ggaaaacgac agcatcgcca
    41161 cgcgcggttc ttctccggtg atggcgcgcc aggtttcggc actggcaagc gcgatatccg
    41221 ccagttgtgc cgccgtcggc tgcggcacca cgctgcaatc ggcaaagccc aacgccggac
    41281 cgctgtactg tggcagcatc aggaaaatgg atgagagcgt tttacagcct ggctgcaagc
    41341 cgataatgcg taatccggca cgcagcacat tcgccgtgga agagaggttg cccgcgatac
    41401 agacatccgc tttaccggcg ctgaccattg cggcggcgaa catcagcgga tcggtaagtt
    41461 tttccagcgc atccggcggc gttttttcgc ccgcgcgggc cagccagcga tgagcaaatt
    41521 cttcccgcat tgcgagattg ccatgcggat ctatcacctg tagcccgtcc atcgccacgc
    41581 cgtgactgag cgcaaactga cgaagttcaa acggattggc gaccagaatg ggcgttgcca
    41641 gaccttgttg atgtaaatat tgcgcagctt tcagcacacg ttgatctaac gcatccggaa
    41701 aaactactct ggcgggcgct cgcaacgcca gttcacgaca acgttcaata atcattgcgt
    41761 ttcccccagt cgttgttgga tctgcttcac cgttagcggc tgcttcgtta cgctcaggat
    41821 catcatcacg tagaccgtgc tggaaaggcg gttcagtgcc tgcaaaatgt ccgggcgtaa
    41881 tacttcaaag ctgcgggtga taaagacctg cgccgctacg gtttcggttt cccgcacttt
    41941 ggtgcgtagc agattcagca acgccgcatc gcgcccgtgg ctggcttccg gcaccagatg
    42001 atcgtggtcg agataacgca gcggctggtg agaaagccga tgcagatctt cgtcagaaag
    42061 cccgacaatc gcctggcagg ccagcggttc ccccagcgca tcggcacgca taatgttgcc
    42121 aagacgcgaa cggatatccg ccagccacgg ctgccacggt tccgccagtt caatttgcag
    42181 ccacactgcc agcgcaatgg tgctgtcgag aacagcgcga aaagccaggc gcggatcgct
    42241 tttggcaacc attttttccg ccgacaggtg agtcagcgta tccggctttt ttgccaccgg
    42301 ctggcggcac agttcgcagc acgcctgcgg atgttcatcg ctactggtca gcccatgaac
    42361 gggctgcggc tgctgctgtt catcgtcaac aaacaggcgg ccctgctcgt caataaactt
    42421 gatgcgcaga tggcggcttt ccagtaactc ccgggcagag ggcgtcaggc gactgtccgc
    42481 agggagatgg atctctgcgc cttcgctgag cgtatggttc gctcttagcc atgcttcggt
    42541 gatgaaatct ttcataggga ttgccagtta gccggccagg cgacatacag gaatttcacg
    42601 ctggatgtcg taccaaattc gatactggag cctttcggga taaacatcac atcgcccgct
    42661 ttggcgatca tagtttcgcc ttcgtggcga acatgcagct ccccttccag caccatgtcg
    42721 atttcgtcgt agttcagggt ccacgggaag aatgcgttct cccactgcat aaaaccagcg
    42781 gccatactgc tgccgtcgtc tccggtcacg agatcagtca agcctacgca gtgtggctgt
    42841 gcgccgtcga aacgaccaaa cttgacgctg ctgccatcaa taactttgat gccgcctttg
    42901 ccggtcaccg atttgaaact cggctgcatt gcaccctgct ccagtgactg tttttccttc
    42961 atcacttttt ccatcagctg cgcgaccagg ctttcggtaa actgaccttc cggcagttgg
    43021 gcgataatgg tttcacggat gcgctggctt tcggttttat cggctgatgc gctggcggga
    43081 acagacgccg ttaccgggat cgattcatca cattcggtga tggtaaaacc caacagatca
    43141 gccacttcac gagcttccgg ggtaataatg ctggcgcgca gaaccacgga cattgccagt
    43201 tcgccgcgcg cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt cacctgcttc
    43261 ctcctgtttg gtgagagatg ccagataatc caccagttgc tgcacgcttt gcgggtcgtg
    43321 gctgtttagc tcgaaaatcg gctcttcgaa gcccgtctca agcaataact ttcgcgttgc
    43381 ggcgacgtca gcatctggca tgtccgtttt actgatgacg gcgatttgcc gtttgctgac
    43441 gccaatatcc agcaacccgg caggcaaccg actttccggg tcattcgcgc cgtggacata
    43501 gatcagcatg tccacatcct gtagcgtggt aattaaggcg tgataccagc ggggatggct
    43561 aaaatattca cccggtgtat caatatcgcc attatcatta aattccacgg tctgtgtttt
    43621 tcttgcgagg gtataattcc cctgcaacgc attaaataac gttgtttttc ctgccccgac
    43681 ggtacccaca aacgcaattc gtttcatgaa tgcctcgtat taacttttgg tcatttcaca
    43741 aagcgtataa tttaataagc gtcctaaacc actgacggtt tgtgataacg cttcttctac
    43801 tgcgcccacc gagccataaa tcaccagcgc gccgctaaaa cggtcgagaa aaccgatatg
    43861 cacatcggcg gctttcaacg ctaaatcacc ggcgatcatc gccgtttctc cgggagttag
    43921 cgtcataatg ccgattgcgc ccgcatccgg aacgccgatt ttcttcgcca gttcctcgcc
    43981 gggatgcgcg atgagatgcg ccagtgtgac ctgtttaccg ggcacaaatt cctgaatgat
    44041 gcgttcttta tccatgagtc gccgcctgtg aaaaactttc gtgtatcgtg acaatttttc
    44101 gtcagagtaa ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga tcactaagaa
    44161 tggagagata tttgctgtgg tgcataaact ttcatatgca acgcatgaat ataaataaaa
    44221 taatatcgag atattttata tactgatttt tttattattc gcaaactcag taataaactt
    44281 tactaatata ttgcagctaa a
//
LOCUS       DADTFG010000017        43284 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.017, whole genome shotgun sequence.
ACCESSION   DADTFG010000017 DADTFG010000000
VERSION     DADTFG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.017"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            97..9717
                     /gene="clbB"
                     /locus_tag="J1138_000960"
     CDS             97..9717
                     /gene="clbB"
                     /locus_tag="J1138_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin hybrid non-ribosomal peptide
                     synthetase/type I polyketide synthase ClbB"
                     /protein_id="HBA8452796.1"
                     /translation="MDNTSGDFPCNKMDTRKQLPLTPSQQGFLFHSLKDKKRSNYHEH
                     FTCIFSQHVDSAHFKWALETLFRKHECFRTDYNWEIDERPCQVVKTDVLPDIYVLDCE
                     QEEIRFLLANDDIIIPVPQDDGIDAIIPQLLQADLKYPFSLKTIPVRAYLIQSTKESA
                     FILSYHHIVMDGWSLSLFIKQLLQLYGAAVVSGVRDDSAIIPSSLKPLVDTLSARRHT
                     FQHDYWAAYLREGTPTCIVPLSQYHTDTEAENNSYVNQTNHVEINLSPDVCQKIQTLC
                     SDYRITPAVIFYVAWGILLQRWCYADDVLFGATISGRNIPIDGIEETLGLFINTLPLR
                     LRDDGATLLQHLQRMHQTLIAHYSNEHDALASIQRLVHKEGHAGDLFNTLVVLENYPV
                     DMTLLSCASPVAIRHLSVHEQTHYPLTLTITQQKGFRFSIAYALNYLTNNMAQALLMH
                     LSYLLEQLVDNPQRPIAALVNLSPCQQAQVLQPYLERMACRDWDSQSNVIEQFHQVAA
                     TSPAQVAVVDELCALTYSELAAQAEQLAAYLVQQGVMVGDTVGIISERRVNTVVAIIA
                     IMLIGAAYVPISPDYPVGRMQEIIDDSGLALLLVHGKPLDALNVAQSDLCAFPVAPSV
                     VFPVITPDSRAYVIYSSGSTGKPKGIAVAHRGLLRLIQGDSPLKVESGETTLLTCPFE
                     FDVSVFEMWSTLLNHGKLVLLSKQALLDINHIRRTIADEQVARAWFTSSLFNSYVAEG
                     ADFFGMLQHITVGGEAVSAWHVNDVMQKYPHLVVTNGYGPTENTIFTTAYRFNGLQPA
                     RVPIGYAVPGTSLYITDLHGHLLPIGATGELVAGGVGVAIGYQNNPALSATVFVPDPF
                     IPGGMMYKTGDYARLLDDGCVDCFGRKDGQIKINGQRIETGEIEQRLLECSGIIEAVV
                     VPYRVRETLHIAAVVCVNDSYDEVEVRGQLADRLPPFAIPESLVVVTEIAKSHSGKAD
                     LAQLRYLLPATQCNAVSTTISEVHSDMEHALHAIWQRVLDRQDIDSNASFFALGGTSL
                     DTIRVKGDIKRQLGLEIDITDLFKYPTLTALAHFLDTAVSPEDAIPTRAVVYSDMPVA
                     IVGMAGRFPGAANIAALWTLVVGGESGLTLFSDEELRAHGVTPDTLKQANYIKTKGIV
                     DDHEWFDADFFGYTPNEAECMDPQIRLLHQCCWQTLEHAGCDPATFTGAIGIYAGLLT
                     SPHWLNAVMQDTTDSAALYKASILNIHSVTALIAHALNLTGPAVTLDTACSTSAVAIH
                     QACIALRNRDCDAALAGGVSIEMPAYRGYEYHEGMINARDGVCRPFDSQASGTVTGDG
                     LGMLLLKRLDDALADRDCIYGVIKGSAVNNDGNNKIGYTAPSVIGQSTVIRTSLRRAG
                     FDSDSIGLVEAHGTGTVLGDPIELRALNEVFGPTPVPFCVVSALKSNIGHLNSAAGVA
                     GVIKTTLALHHQVLPPTAHFRQLNPAIDLSRSALYVNQQVQPWPSTRPRRALVSSFGI
                     GGTNASIALEAHQHEDDPSATGVRDSYLLLFSAKTPAALELRVASTLEYVKHGVGVRL
                     PDVAYTLQTGRTAFDHRRAYLVSRGSKIDLSCATILQAEIFNGQRTTAEICFMFPGQG
                     SQYHGMASALYAHQPMFRQHMDRCFAAFQRYSTVDLKALLFDDEDTRDIDQTQFTQPA
                     LFCVEYSLARTLIDLGITPDSMIGHSLGEYVAACIAGVFTLEDALHVIEARGRLMQSM
                     RPGSMMAVYLSREQLTPWLAAERGIELAANNSAHFCVVAGEQAAISRLSTRLVEGGIQ
                     HRRLKTSHAFHSAMMTPMLHDFAQLLGQIPMHAPHKRFISNVSGTWITEEQATSPDYW
                     VQQVRNAVLFSEGAAQLLVQPTLFIECGPGNTLSTFIQGHNQYSDQPTLLTLRKANAA
                     IDDEHMLHRTLAALWVRGENIDWRRFNQTALGKHIPLPDYPFEQTYYYRYGAALSGYR
                     QYPNPLRRPQDEWLQRVLWRMHDTSLREAFYAPGELIIIISADGDKLQQTLMSSGVDS
                     ITMPLPISSEDDVWDNDRILTHFHDICALLAHKTYRQLHCLYAPGAEAGSSLTQSLSG
                     LYRVARWCMHSTTPLASLTVLTHGAFRVQEEDNPEPTLAALSGAVNVFAQELHPTEVR
                     LIDIDAQSSDENLNLLTQRLAPKQETVMALRQGMLYLRRFIPTRLLAHLPPQTGCIPG
                     NVLWIIGGEKGIGRMIGEALAQREGVRVVLSSRTGYHHEAVQQDALDVIHCDVTQAEA
                     VRACLATLLERYGRLDGVIFAADATTTLTLHQLSESALRDTLTVKERGTANVLHALAQ
                     RNLLDERLLLLFCNSLAAVNAEIGQTGYATASAYLDALAQQLRTRYKVNALSIGLDAL
                     REQGMLLDAINGSEYDVLRGLRPLMTGTLLQAYKQQGADTSYYARLSPESDWLLDEHR
                     ISGIATLPGTGYLALAYEALRHYFVQDQICIDELVFLAPLTVMDNCSVDVFVDISPNG
                     QGVSVEVKSMTERFSGTLTTHARGRATRLMVDDNVVCDLTGLMREMHTITPPTKELSS
                     THFHYGPRWHSVQQLYGNTAQTQVFATLALPTVAANDTIALHPALLDIASSVAEQLPG
                     FHTDSVPFLYQDLRLYRPLPNTLHVALTVNRHDEEGDSYAFTLYDMAGEMVARCAAMV
                     KRKVQLHIQDVDDDTRLRVPSADNYQLRLAAEGEGAGKLALCPTPRLALGDSQVEIEV
                     LATGLNFKDVLFTTGLLRQQPGEAPLQLGLECAGRITRVGKNVTEFAPGEDVMAVLNG
                     GFVQYARVESDCVVRKPAHCRIEQAAALPIAYLTAYYALVVRANLQPGERVLIHSAAG
                     GVGLAALHIAKRCGAQIFATAGSEQKRDYLLSLGVHAVADSHDEQFAATLLTASDGQG
                     MDVILNSLTGRLLDASLALLAPLGRFLELGSKDIVEDKALPMRFFAQGGTFIPINFHA
                     AHGAFSRYLQQIVAWIDDNTLPLLPCKSVPLPEVARAFATLTTPQHIGKVVVTHRTAA
                     GMDRLNAMIAERRLGGYALSMSNAEVMRQLWPILNTRSPWAQLLLSPRAIDRLARGNR
                     VDRGVPSAANDTITQQTVKKRPRPEIGVPYSPATREVERVLCQILEEYLGLDRVGIDD
                     NYAELGATSLDMVQLSGQMARHYPQVSVVSLYNHATVRQLATFCQPPEGESNAPSPQP
                     AVQTNTRANQIAKRALQIAKNTARSHTSLH"
     gene            9758..12358
                     /gene="clbC"
                     /locus_tag="J1138_000961"
     CDS             9758..12358
                     /gene="clbC"
                     /locus_tag="J1138_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbC"
                     /protein_id="HBA8452797.1"
                     /translation="MEYASEMNGMEIAIIGMAVRFPQSRTLHEFWHNIVQGKECVTFF
                     SEEELLAEGVEQSTLDNPAYVRAKPYIEGICDFDAAFFGYSHKEAQTLDPKSRVLHEV
                     AYHALEDAGYAQRTSDLITGVFVGASEDVDWLRRSLSQIGGDALNRFESGIYGHKDLL
                     AHLIAYSLNLNGPVYSLYTSCSTSLSATHIACRSLLFGECDLALAGGITIDLPQKSGY
                     FCQQGMIHSTDGHCRPFDSQASGTLFGDGAGVVVLRRLEDALAAGDRIYAVIRGSAVN
                     NDGKQKIGFVAPGHEGQKAVICAACHLAEVSPESIGYVETHGTGTRIGDPIEFAALTE
                     AFDTSHRQYCALGAVKANIGHTHAAAGVAGLIKTALVLHHRTIPPLANYQMPNSKLDL
                     AHSPFYIPIQPQEWPASRMPPRAGVSSFGIGGTNVHMILEGLNPAVRDDHDQVRAPVF
                     IPLSAPSFEQLDELTQQLTPLLATLDASTLAYTQQVARPVFDCRRVIQVENDGTQAML
                     ASLDNLMPDAPWGLHCPDLRTTNDCTYAQWLAHSAHYQREATALTALLDGMNIPPAYC
                     HAETWAAQANSSLLIRGCQTIAALKTWMNLLPTLTLLSGAGTGLLPAAAASGMIATQD
                     VLHLLWEMEQKALHLWLPERHEPIPGYVLAWQGNPITDAQRNDRGFWSEALLADTREL
                     GEGVHSINWVRLPPEIREDVDVLRYVAQLWCAGINVDWAVWYGTPLPQRGSASAYPFA
                     HNHYPLPGRVMGSVETQPEAGPETHHPYQARPVLSVPFVAAHSRGMQYITGLMELLLE
                     ISPVGVDDDFFELGGHSLLVTQLTSRLERDFNVHIDLLTLMENPNPRNIYAHIAAQLG
                     GEDNLEIACQ"
     gene            12368..13237
                     /gene="clbD"
                     /locus_tag="J1138_000962"
     CDS             12368..13237
                     /gene="clbD"
                     /locus_tag="J1138_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis dehydrogenase ClbD"
                     /protein_id="HBA8452798.1"
                     /translation="MMNVAVIGAGVMGTGVAHNMAQYGISTNVVDISQSQLDKCRQMI
                     EANLRLYNFHPQHKKKTHSTAEIMENIRFTTELDDIVECDLVIENITEDIEKKNALYT
                     RMNTICGASTVFGVNTSAISITALSKLMRHPENVVGVHFMNPVPLMHTVELIRGVHTA
                     ERTLNIFHHLFAQLNKTGIVVNDSPGFVTNRAMMIFVNEAIFMVQEQIARAEDIDTLF
                     KTCFGHKMGPLQTADLIGLDTILQSLQVLYESFNDDKYRPSFLLKKMVDAGYLGVKSG
                     QGFYRYQQTYAEQ"
     gene            13267..13515
                     /gene="clbE"
                     /locus_tag="J1138_000963"
     CDS             13267..13515
                     /gene="clbE"
                     /locus_tag="J1138_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis aminomalonyl-acyl
                     carrier protein ClbE"
                     /protein_id="HBA8452799.1"
                     /translation="MKKQDMKAAIREFLSRSLRGHTLNDDDDIFSLGLVHSLFTVQII
                     LFIEKNFQVELEVSELKTEQIATVNKIVELIQRQTGLE"
     gene            13519..14649
                     /gene="clbF"
                     /locus_tag="J1138_000964"
     CDS             13519..14649
                     /gene="clbF"
                     /locus_tag="J1138_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis dehydrogenase ClbF"
                     /protein_id="HBA8452800.1"
                     /translation="MCTENYELAQQEAVLFAKQHLALAAQNIERQQFIVPDIISCVAQ
                     AGYLGASIPQKYGGRGYDSYQLCALHEVMAGVHGSLENLITVTGMVSTLLQRVGSAAQ
                     KAHYLPKLATGELIGAIALTEPNIGSDLVNVETELQQDGDGWRLNGKKKWITLGQIAD
                     FFIVLIHCGNQLATVLIDRNTDGFTITPLNDMLGLRGNMLAELHFNDCRLKEDALLGP
                     LTPGVPLAVNFALNEGRFTTACGSLGLCRAAVDVAARYIRQRKQFKRRLFSHGIVQHL
                     FATMLTQTRSAQLMCFSAAEYRETLHPAMINQILMAKYVASKAAVDVAGKAVQLLGAN
                     GCHADYAVERYYRDAKIMEIIEGTSQIHEIQIAMNYMMGSEA"
     gene            14646..15914
                     /gene="clbG"
                     /locus_tag="J1138_000965"
     CDS             14646..15914
                     /gene="clbG"
                     /locus_tag="J1138_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis acyltransferase ClbG"
                     /protein_id="HBA8452801.1"
                     /translation="MTKDVALMFPGSGSQYVGMARWLYERYPQVRTLFDEASQITERD
                     MAALCLSGTLVQLAEPTAMALAIYTTSVAHFVAWQQFLAQTGVHVNLRYMLGHSLGEY
                     AALTCSGALSFSQALALVAMRSRLASEIAREMDASTTIIKQGNQALVAAACEVAERET
                     RQQVGIACFNSPQQFMLSGQNSAIIAAEQYLLDHDRQVEVVPLIGGVPYHSPLLKPCG
                     QQLRKALDRCEWRRPCCPVISNVNAQPYPDTVTPVQWLEQQLSQPVQWQRSLTYLTGH
                     LSPIAIEIGPQSVLKNLLLENRYPAPVYAFDNRHDRAQLALVLGDNMAVKTDPEAVRR
                     QRITLLTNALTATRHHRAADVAASAQLKELLSRFFERIQQIEQRGTSSEEDIAFLHEL
                     LEQGFQLKGSSQAEIDACHARLASDNGGQA"
     gene            15962..20758
                     /gene="clbH"
                     /locus_tag="J1138_000966"
     CDS             15962..20758
                     /gene="clbH"
                     /locus_tag="J1138_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbH"
                     /protein_id="HBA8452802.1"
                     /translation="MEQQGIMRQLPTDDQTIVDYLYRIAGEYGEKAAVLMGDAALSYH
                     DLNARSNQLAHYLRGLGIGEDRVVAIRLPRGMAMLIAIFAIVKAGGAYLPLAYNAPRS
                     RIENILSNSGAVCLIGTDDGDRWPIPRVEIDSAAVSAMPTTDLRYRPHARQLAYIIYT
                     SGSTGVPKGVATEHAALLNRIVWMQNAYPISSQDVLFQKTVYTFDVSVWEMFWWAMYG
                     ASVVLLPSGLESDPRTLARLIQRHRVSVVHFVPSMLNLFVEYLEMKQDPRLTASLRLV
                     FSSGEKLTVHSVARFYQSVAQGDLINLYGPTEAAIDVSHHRCLRGYDYDDIPIGQAID
                     GCRLYVLDDHGNPVADGEEGELYLAGIGLARGYLNNVALTDRCFTIHPTLRHLGKPER
                     LYKTGDLVWRDGESQQIHYIGRNDFQIKIRGLRVELGEIEAHAMRFPGVQQAVVVADQ
                     DDPDNQLIYAFVVSSVPLNLAALMDALSKNLPAYMLPNRLLAMSELPLSDNGKCCRKT
                     LLDLARAYSASRVDLRETPAVRYLPLSSAQSSMWFMQQLAPHTALYNNPTALLLEGEL
                     DRTRMDGAIRQLMSRHTLLRAMAETHNGQPVLAVPQCVSSQALLTIVPLPSVSDDNAL
                     QAMINQRAAHPMPLTSGTPLCRFELLTIDDDRSVLLIHLHHIISDGWSKGVLLRELQA
                     AYNGESLTPEPLLEYADYMEYQEEWRQSDAYQDAMRYWQNTLAGTLPILDIPTDQPRQ
                     KVARYQGAFVAFALSANTCERVLAAARAQRVSLYNYLLTAFVLLLHRNARQQEYIVGM
                     PIAARLTKEQEHMIAPLVNVLPLRLPLDEAASFSELVQTIRGILFAAFRHQRLEFTDI
                     VRAVNVDRSAGHFPIYQCMFQLDNMPLASPTLNGVNVTPLLLDTSASQVDISLSMQHI
                     DGRITGTFEYDAGLYSADRIQHLVAQWRELLDEASSQPTQLVRDLIRFTPREHAWLAR
                     HNATEVALPPVDNLLALVLPHCQQRPTQVALRHADDAMTYGELQQATMQMCTWLRAQG
                     VKRGESVALQLPFCFELIIAQLAILSLGASYVPLDGNAPAARNALILAQATPCMLLVA
                     QPLESPHGLTIPWVLVPDWRSLLTEIPNLPVSVAPDALDCDAVVIFTSGTTGQPKGVR
                     LSQRNLVNLTASFISSYQVTHQDVLLPITSVASASFVGEVLPLLAAGGTLVLAQKAQS
                     LDSDALIALLASQRVTILSTTPSLSASLSVLAQSMGSLRLFLCGGEALEYEQIAPLLP
                     HMAVVNGYGLTESGICSTYFPVAKRREQETGALPIGRPIQNTQAYVVDAYNRLVPPGA
                     CGELCFSGLGISPGYLDARQDPERFVELPEYPGVRVLKTGDRARWATDGMLFYLGRQD
                     RQVQIRGYRVELGDIESLLKQHPDIADAWVDVRRNAAATPLLVAFYCSVNGVALDAQQ
                     LRVWLSLRLPLHMLPLLYVPLSAMPLGVNGKIDPQCLPLVDLRQLEGPGEYVPPATEL
                     EQRLAEIWQQLLGLERVGTTTNFFDLGGHSLLLVQMQQYIGQQCGQHVALVDLLRFTT
                     IKRLAEFLLAPDAAQGTTGDQTQLRAAKQRLAFGHTRWAATTDSHH"
     gene            20808..23840
                     /gene="clbI"
                     /locus_tag="J1138_000967"
     CDS             20808..23840
                     /gene="clbI"
                     /locus_tag="J1138_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbI"
                     /protein_id="HBA8452803.1"
                     /translation="MAENDFGIAIIGMAGRFPQADTVQAFWENLLASRECISFYSDEE
                     LLAMGISPEFVQHPDYVKAKGEVADIDKFDAAFFGIAPREAELMDPQHRVLLETAWAA
                     FEDAGYVAADYPGDVGIFAGKSMDSYLMLNLMPHFKRVFSSGSLQAAIGNDKDSITTT
                     IAYHLNLRGPAITVQTSSSTSLVAVCVACQSLLTWQCDMAIAGGVTLGPPAKTGYLSQ
                     EGGITAADGHCRAFSDNSSGFVPGTGAGLVVLKRVDEALRDGDNIYAVIKGFAVNNDG
                     SEKISYTAPSVDAQARAIAQAQRLAGLTPQDITYVEAHGTGTRLGDPVEFSALSQAFA
                     GASQKQYCALGSVKTNIGHLDTAAGVAGLIKTALAVQQGIIPATLHFERPNAQIDLTN
                     SPFYINTTCQPWQPESGIRRAGVTSLGMGGTNAHVVLEQAPAVDLQARAPVPAYSILP
                     FSAKTDSALSSGLARFADFLQHESLPDRRDLAWTLSQGRKAFAHRAALVTRDLHAAGT
                     LLQQAATAPFARGVAQTQLGLGLLFSGQGSQYQRMGHQLYQVWPAYADAFDRCATLLE
                     REYQLDIRHELFRAEVSLAQGERLAQTCLTQPLLFSVEYALAQLWLSWGITPTVMIGH
                     SLGEWVAATLAGVFSLEDALRLVARRAELMHQAPSGAMLMVALPEAQIRALITAPLAI
                     AAVNAPDYSVIAGPTSEILAVSQRLTEQNIINKRLHTSHAFHSSMMQDAAQALRQAFE
                     NVRLNPPTLTIISTVTGAHVSADTLTTPDYWIEQMLMPVQFSAALQEAQATFDVDFLE
                     IGPGATLTQLTNGHALGDRLAFSSLPAGARSSDEHKHILDTVAALWVRGHNIDLSAFA
                     GEQPRRVSLPTYAFDKIRYWVDSPEEQRSAVTPVADAGSVIPSEPSVRRQPRPAFSVP
                     YAAPESKTQRGLVAICEALLGIDGLGIDDNFFEAGGHSLMLGMLLAQVQERFAVTLSF
                     FDVMEDASVRALAQLVEQEQQDDGGSALAVLVNDMINE"
     gene            23884..31206
                     /gene="clbJ"
                     /locus_tag="J1138_000968"
     CDS             23884..31206
                     /gene="clbJ"
                     /locus_tag="J1138_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbJ"
                     /protein_id="HBA8452804.1"
                     /translation="MTIHHAALARMLPAEKKEKLLRQLAQSGVSPSRIPIIKADPAQA
                     IPLSFNQERLWFLQKYDSTATNYNLYVVYRLHGVVDMPMLTEALRHVQARHAILRTRI
                     IVRNDRPCQVIDDASSLVLDTVTLAAQAPTSALDAVIQQVINTRFDLARGPLWGVTQI
                     IQPDQGCHLVFCAHHIIIDGISLRLLFDELQQQYARLHAGNETSLPPPPLQYADYAFW
                     QREWFQDTLLANELAYWRARLQDAPLLSTFPSLHPRPAQPSTHGSRFSITLDETLSLA
                     LKHVARTQETTPFVLMLTAFQLVLMRYAQQQRLVIGMPVSGRIRPELQSSIGYYASTA
                     VIYTDFNGVEVGREALQRVKASVKETQGRQQLPFENLVNMLDLPRSLSHSPLFQILYI
                     YHNHVTPRAFTLAGAYWEQVTYHNQTVKYDMTVEVFQNDATFDVSFEYDLGLYDADVV
                     KQIAEALRQHCLSLTSSLETPIGAIPLHAPETATPRRDPLNATNVPWLGPQDVLRIIE
                     QRCVQHPKQLAIQQHDGTLTYAELWARVQFIAMRFRAHGIQPGDRIGVLLPRHRDVIA
                     TMLATWFVGACYVPFDIHQPAARLQRLMQRARLVCLVVRQPGEWGEIVQLSLPELMQD
                     MSNAIRYSTPCALLPDMQAYLLFTSGSTGEPKGVCVVHRGLLNLLLDMQRTFAVGSQD
                     RLLSVTTPTFDISFLEYLLPLISGASLYLTEAERAADSFRMIPLIADYRPTLMQATPS
                     FWHGLLMAGWRGDPELCVLAGGEALPTKVAEELLRCCGSLWNLYGPTETTIWSLKSQI
                     TQAENITLGAPIANTRIYILDNEGHPVPQGVDGELYIAGDGVAQGYDGQPELNAQFFL
                     SEPGVPGGRMFRTGDLVRSDAQGQLFFVGRKDSQIKLRGYRIELGEIERTLARHPHVD
                     AAVVACIERAPLHKALAAFIITSEPPSLFEQLKNELRQQLPDYMVPTLWQRVADFPNT
                     DNGKIDRKRLAENFVADSSLVSPQTQALSDTEQMLLALWMRYLPIKNVDPECDFFRLG
                     GHSLLAVTLVAEINRTFHCALTLKDIFHYSTLRALSARIAQQSITDAAASQDDWVIVH
                     DPEHRHQPFPLTDVQRAYWLGRQTGATSIATHIYHEFDVEHFNVTRFTHAVNALIARH
                     EMLRARVLPDGTQQILAQVPAYQLEQRDLSALSPNARNDALMAIRDRLSHHVHPADRW
                     PLFDFSYSACTAQHGRLHFSLDLLIADALSMRTLQQELMMLYREPHVSLPLLPFSFRD
                     YVQALLVEQASEAYARDQAYWQRALPQLYGPPTLPVQGDLAQLSAIRFVRRRHRLSAH
                     NWGVLSALAQRTRITKTALLLTVFSQVLARWSLSPTFTLNLTLFNRPQGYPNAEAVIG
                     DFTAVSLLNVCYDSQHSYAHNAQRIQVQLWEDLEHRRFSGIRASEALIHSGRFHAPMP
                     VVFTSMLDIDGETTAQDPRDTTRFTLCPDANITQTPQVWLDHQVIELAGELHFNWDAV
                     EQLFDTTLLDQMFGAYCHALQALVAMPQSWWGVNSSLALPTVSAPVTQAPAPTALLHH
                     GLLRQAALTPQETALISPIRELTYRQLSTAADHVARALLALGVQHGDRVAVVMEKGWQ
                     QIAAVHGILRLGAVYLPVDPVLPPQRRQLLLTVGEVRVQVTQPGLTQLEPSLPVLIID
                     DGMLDTPAAPLPEVAGDVTDLAYIIFTSGSTGTPKGVMIDHRAAMNTLEDINERFGLN
                     AQDRVFGLSSLSFDLSVYDAFAPFMVGAALVLPEAGREKDPRHWQTVMAHGHVSVWNA
                     VPALMQMLCEYHSGDRMSYPTLRLALLSGDWIPLTLPEQMRERLNETMDIISLGGATE
                     CAIWSVYYPIGEVESTWTSIPYGRGLRNQPVYVLNAQLEECPVGVEGEICIGGMGLAQ
                     GYLNDAEKTAASFVWREASGERIYRTGDRGRYFADGQVAFLGRNDTQVKVNGYRIELG
                     EIERCIARHPDVEQSVVVAVGNSQHRRLVAFAKLHDRHQAQALQAKEAEAAALAQGII
                     VNPAQRLAFKLKEPHIRALDGLGIALTAPADSTRYIKRRSYRHFSAQKTTLAQLGQLL
                     SGLGQMRLPGLPFAKYAYASAGGLYPVQTYVYLHPDKIEEGVSGIYYFDPRQSCLMPV
                     APEVELNSGFHAGPNQSIADRAAFTLFMVADMAVISPFYGQEAAWHFSVMEAGTLCHL
                     LEEDAPRYGLGLCQLGMADFSAVASHFQLSPHHRYVHCTVGGAIGQEAASAAALLRDF
                     STYEKPKETAAPLDMQSYKDAMLRGLRQQLPDYMVPSDLMLATDFPLTANGKLDRQKL
                     QLQGEQIAHQRDGVGPIQVDSALQQRLVALWQEVLGVSHVSAEDDFFSLGGSSIELVR
                     IQQALEAIIGQEIPIVDLFRLPTIADVARYLDEQLHNLPAAHDIVLAQAEVSQVSAAR
                     ENLALRRKRAQQGEKGDE"
     gene            31199..32662
                     /gene="clbL"
                     /locus_tag="J1138_000969"
     CDS             31199..32662
                     /gene="clbL"
                     /locus_tag="J1138_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis amidase ClbL"
                     /protein_id="HBA8452805.1"
                     /translation="MSEQSYRSAGTLLAQLASGETTSVALVNHYFSRMAQFNKPLNAV
                     VQQHYALALEAAARADRERLEGRARGVLHGLPCTVKESFDVQGWLTTSGAHYLKDNRA
                     TQDAPSIARLRAAGAILMGKTNVPMMTADWQTYNDLYGTTHNLWDRQRSPGGSSGGAA
                     VAVAADFTPVEFGSDLFGSLRIPAHYTGVYAHRCSLGLMSVRGHVPGGGPQATDEPDL
                     STAGPMARSAADLRLMMRALSTFWVEPPRIPDFSRYQAKANYRVCTWFSAPHHEIDQQ
                     IAQRFQSFIDKLRAQPGVEVDDAMPADIDPDALFDIAVKLSGRLVSTALNGRQRLTAG
                     LAALGFRLVGKLADVPEGITSYYQGMLKDSGEQRNTDKLRHEYSRVIETLFARYDVLL
                     TPVSPVLAFAHMQQPVRKRKLIVNGEPQDYNEHLFWNMLATVFGLPATVYPLAKTMDE
                     LPCGIQIISGHFHDDVTINFAEFCESISGGFTVPEGY"
     gene            32724..34163
                     /gene="clbM"
                     /locus_tag="J1138_000970"
     CDS             32724..34163
                     /gene="clbM"
                     /locus_tag="J1138_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precolibactin export MATE transporter ClbM"
                     /protein_id="HBA8452806.1"
                     /translation="MTVNKLEPDNGTPNDELFTVAGMFDGSLYKLLLRMALPMFVGML
                     TQVTYAIADIFWLSHIDVTNSGIIAGVGLVFPVGMGLFAIANGIQIGMGSLLSRAIGM
                     QRLDRAQRILSVGIIIALFFAIVITVLGYVYAQPLLRSLGATKSIIGYATEFYYYSLL
                     TVFSIMLIGVMMGLFQGAGKIMVIMKASLLGALVNIMLDPIMIFVFDFGVKGVALASF
                     LAQLSMVAYFIYTLMGLHIGLSIRIALRPFSWKIYREFLSVGMAQMLMQLIIAVGIVI
                     YNFFIVRLDVNAMAAFTLTGRIDYFIITPMLAIATALLTVVGQNWGHGNVTRTLNAYW
                     AAVALAFSIVLVLAVMHIVLAPWMYPLFTRVVAVSDYAVLQTRIMALALPFVAISLLA
                     SEYYQAIGKPWYSVLLTLMRHVFISVPVVYLLAIVLEMRITGVYFGAMSGTFVAALLA
                     WRLLRLSPRLLRWNQEAVRSQHLDMEVAP"
     gene            34160..38527
                     /gene="clbN"
                     /locus_tag="J1138_000971"
     CDS             34160..38527
                     /gene="clbN"
                     /locus_tag="J1138_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbN"
                     /protein_id="HBA8452807.1"
                     /translation="MMSGNPLSWPQEQCHIIDQLYPYSAVNIIGGVVTIEGIVDLPRL
                     HAAIQSAIRQFDALRMWFVMGEESEVVSQVQPYHWRDIRHLTFSPDYDKENLRPAAIE
                     TFVDEWFRQPFTLLAHDLFEFVTFTCGEQYSGYLFKAHHGIADGWSMALLSNHVKRAY
                     EQQDVPDDASPAYSAFLAQQQSYQASTRFAVDRGWWRDYIDEYRDCFPDSSPIVTTEG
                     ISCSTWLEPAMINRLYRLCNRYGCTLNTLFIALFALYRARVWGEEKGVIGVPLANRHT
                     REARRCFGMFTNQLPLAYRLVRTERFCERVAFFQRELKRGFKHSKYPITLFNQDLAEQ
                     GGGKLRAFDYCVNYYNFTYERHIAGAAQRVESYYSGEQSYKLQIVLQTVNNHKESLRL
                     SLEALRSAFTPHQLTAMKNGLLDLVTALDRQPDARLGDLEVYPAPHVALACGSLKPSF
                     TSRFAAQVVEHGDRTALIDNEQSLTYRQLDDAVERVARYLRQQGIGRGQVVGIIAEHS
                     AQTVMVIYGILRCGAAFLPLNPALPTTRLYAMCRKAQVAHILYDPAMHELTQALAFPA
                     SSLLQALATSALAREPWPAIEPQDLAYVLFTSGSTGEPKGVQVSHGNLANYLHFAAER
                     YFTAQDRAALYSSLSFDLTITTLFAPLCVGASISVCRHAESETLLRMAVVDQPNTVIK
                     LTPAHLRLLCAAGISSEQIRTLVVGGEDFKRDLARKAAALFPQAVIYNEYGPTEATVG
                     CMIYRYTGQETLPSLPIGMAIDGCQVAICSPWGCPVPEGETGELVIYGASVTQGYIDA
                     PQQTAAAYLKDTNGVMIGYRSGDIGYAIAPNTLVYQGRKDDQVKINGYRIELCEIEQA
                     LLSAPQVESAAVAVIDDVQGQHSGLLACVTPSSVDVATVMQHLRQQLPTYMQPKQCCA
                     IAQLPLSHNGKVDVRQMVATVRNTAPASGSERLGDAAIRHSVRVCVEGALEQTEFDDN
                     ENLYVLGLDSIKSIQIAAQLRHHGWTMSAVQVMECGTVNAICEFLASHTTVSQLAQYA
                     HNTRIDLPALRWFTQLALPVPNVYNHVIVLKVLPGCPLEQLHNRLHTLIQQQPALHSA
                     LDAEGRLLVCDPNVCYPNEVLTEYSTAQWTLAEVIAQCNSMLDVTNGRVFTAALLHAP
                     QPASSTLVLCAHHLCVDMHSWYLILSTLDAVSTVNGTSNSGLHRWNDYLASKTVDSAT
                     HESWRTVCQTLPLHFPPVSLPDDSLPRTRAWREDFRHPCVRRLFESSGNTAYSAETYV
                     LTALALVLRYYSEEPWCRIEMEGMGRGCWPDEPDVADTVGWFTLFYPWAIPLHGDMAT
                     LLSAIASDLAKRTHGGGDYGLLQMRHAPEDSLAQGIRMNYIGVQAQPSLRYFHIDHFN
                     SDIYTAPENALGCVLEFNIARSAADGLSFHCRFDPTRIALNDVQLLLARYKNSLTDLD
                     AWLCQHSATLTGAPTLWTL"
     gene            38558..41017
                     /gene="clbO"
                     /locus_tag="J1138_000972"
     CDS             38558..41017
                     /gene="clbO"
                     /locus_tag="J1138_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbO"
                     /protein_id="HBA8452808.1"
                     /translation="MAKDDFTCGSLDIAIIGMSGRFSGAESVPEWWDKLLAGEEFTQP
                     TCTEDDNGNPWIRLRNIITAPYDFDAAFFNIPPGEALLMDPQQRIFLECCYNALEHAG
                     YIPTQLKRVGVYGATYANNYFIDRVYPYLKMSGDHHYLQAQIGNEKDYLCAQVAYKLG
                     FTGPAVSVQTACSSSLVAAYLACEGLLTFQADVALAGGVTLGFLQAHGYSPQGDKLVS
                     QDGHCAPFSAEATGTVYSSGAGVVVLKRLEDALRDQDRVYAVIKGGAVNNDGGRRLGF
                     VAPSVEGQVEAINTALAAAEVVPTDIALIETHGTGTPLGDEIELEALHRVFAPACAPH
                     SIQLGAVKANLGHLGVASGIVSLMKTALTLYTGLVPPQINLVNKHKKLLQPASPFYLS
                     DVVTSVPQTKRIHATVSSFGLGGTNAHLVLQNWCETPAQAVQENERRLFFFSAKTPLA
                     LRQQLDAHYHALATYAEADKDRIAYTLAQRRAHFPYRCALAADSVVALRASLAKLRDA
                     DMSFTPINMETTLVFLYPDRDDKLESALTHLLACQPNLRQRHQRLSQDVAQICEPADW
                     TPALRQFIQQVSLSEWLIEQSISPVQHIGYLTGAAAAQYVARIISLENAVQQVIVAET
                     TPEQTLAGNSELSEILANLAVTEGTLMLEIGRAGTFSILYHQHAQWVGQTVFSPMLNT
                     DTPEDILPLLGTLWQRGVTICLPEMPAVQTIGLPGYSFDRVRYEIQSSDARENAMLPV
                     SYLSVSDFVEKTWRSLLCIDHYDEHAVIFEYGATSMHVISFVDSCNHIYKIGLTAADI
                     YARPAIREHSEFISECVDGIL"
     gene            41030..42544
                     /gene="clbP"
                     /locus_tag="J1138_000973"
     CDS             41030..42544
                     /gene="clbP"
                     /locus_tag="J1138_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precolibactin peptidase ClbP"
                     /protein_id="HBA8452809.1"
                     /translation="MTIMEHVSIKTLYHLLCCMLLFISAMCALAQEHEPIGAQDERLS
                     TLIHQRMQEAKVPALSVSVTIKGVRQRFVYGVADVASQKANTLDTVYELGSMSKAFTG
                     LVVQILIQEGRLRQGDDIITYLPEMRLNYQGKPASLTVADFLYHTSGLPFSTLARLEN
                     PMPGSAVAQQLRNENLLFAPGAKFSYASANYDVLGAVIENVTGKTFTEVIAERLTQPL
                     GMSATVAVKGDEIIVNKASGYKLGFGKPVLFHAPLARNHVPAAYIHSTLPDMEIWIDA
                     WLHRKALPATLREAMSNSWRGNSDVPLAADNRILYASGWFIDQNQGPYISHGGQNPNF
                     SSCIALRPDQQIGIVALANMNSNLILQLCADIDNYLRIGKYADGAGDAITATDTLFVY
                     LTLLLCFWGAVVVVRGAFRVYRATAHGPGKQQRLRLRVRDYIIALAVPGLVAAMLYVA
                     PGILSPGLDWRFILVWGPSSVLAIPFGIILLAFVLTLNHQIKRILLHNKEWDDE"
     gene            42537..43259
                     /gene="clbQ"
                     /locus_tag="J1138_000974"
     CDS             42537..43259
                     /gene="clbQ"
                     /locus_tag="J1138_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
                     /protein_id="HBA8452810.1"
                     /translation="MSNISLYCLPYSGGSAAMYYKWRSVLSDNITLRPLEPAGRGTRI
                     RQPLCLTMVDAVADLYQQFVKHYTGGDYAIFGHSLGGIMAFELVHYILDHGHDMPCAL
                     FFSGCRPPDRASHEVILHTLPDQAFMEEIVKLGGTPVDVFRNKELMTIFTPIIKNDYR
                     LYEQYVFQAKARTLTCPIVLFHGDADNLVMQDELLAWEKFTTRKTRTIIFPAADHFFV
                     DKHFEQVVGYVNQTIESLEIVG"
BASE COUNT         9584 a        11068 c        12270 g        10362 t
ORIGIN      
        1 tatgaatacg caataaaaaa ctatacttgc ggataggtgt gccatttaga ataatcatgt
       61 taaataatct ataaatccga taataaggtg atggttatgg ataatacctc tggagatttt
      121 ccatgtaata agatggacac gcgtaagcag ttaccgctaa caccaagtca acaggggttt
      181 ttattccatt ccttaaagga taagaaaagg agtaactacc atgagcattt tacatgcatt
      241 ttttctcagc atgtagatag cgcccacttc aagtgggcgc tggaaacgtt atttcgaaag
      301 catgagtgtt ttcgcactga ttataactgg gagattgatg agcgcccttg tcaggtggtg
      361 aagaccgatg tgttgccgga tatatatgtg ttagactgtg agcaagagga aatacgtttt
      421 ctactagcta atgatgacat tatcattcct gtcccgcagg atgacggtat tgatgctata
      481 attcctcaac tgctacaggc tgatttaaaa tacccatttt ccttgaaaac gatcccagtc
      541 cgggcctacc ttattcagtc aacgaaagaa agtgctttta tactatcata ccatcatatt
      601 gtgatggatg gctggagctt atcccttttc attaaacagt tgctccaact ctatggagcg
      661 gctgtggtca gtggtgtgag ggatgatagc gccattatcc cctcatctct gaaacccctt
      721 gtagacacac tgtcggcccg acgtcacacc tttcagcacg actattgggc tgcatatctt
      781 cgggagggaa caccaacttg tatcgtgccg ctgtcacaat atcacacaga tactgaagcc
      841 gagaacaatt cttacgttaa tcaaacaaat catgtggaga tcaatttgtc tccggatgtg
      901 tgtcagaaaa tacagacgct atgcagcgat tatcgtatca cccccgcagt aatcttctat
      961 gtggcctggg gcatcctgct acaacgttgg tgctatgctg acgatgtgtt attcggcgcg
     1021 acaatatcag ggcgaaatat accaattgat ggtatagaag aaacactagg gctatttatt
     1081 aacacgttgc cgctgcgtct gcgtgatgat ggggcgacac tgttgcaaca cttacaacgt
     1141 atgcaccaaa cactaatagc tcactacagc aatgaacatg atgccttagc cagcatacaa
     1201 cggttggtac ataaagaagg tcatgctggg gatcttttta ataccttagt ggtgttggag
     1261 aattatcccg ttgatatgac attattgtca tgcgcgtcgc ccgtggcaat tcgccatctc
     1321 agtgtacatg aacaaacgca ctatccgctg accttgacca tcactcaaca gaagggattc
     1381 cgtttcagta ttgcatatgc ccttaactac ctgaccaaca acatggcgca agcgttgctg
     1441 atgcacctga gttatctgct tgaacaactg gtggacaatc cgcagcgccc cattgctgcg
     1501 ttggtaaact tgtccccatg ccagcaggcg caggtacttc aaccctatct ggaacgcatg
     1561 gcatgccggg attgggatag tcaatccaac gtcatcgaac aatttcatca agttgctgcg
     1621 acttcgccag cacaggtcgc agtggttgat gaattgtgcg cgttgaccta ttcggagttg
     1681 gcagcacaag ctgagcagct cgcggcttat ttggtacagc agggtgttat ggttggcgat
     1741 actgtaggca taattagcga acgccgggta aacaccgtgg ttgccatcat cgccatcatg
     1801 ttgatcgggg ctgcttatgt gcccatcagc cccgactacc cagtgggtag gatgcaggaa
     1861 attattgatg acagcggctt ggcgctgttg ctggtacatg gcaaaccgct agatgcatta
     1921 aacgttgcgc agagtgacct ctgtgcattt cccgtcgcgc cctcggtggt atttccggtt
     1981 atcacaccag attctcgcgc ttatgtgatt tattcatcgg ggtcgacggg taagccaaaa
     2041 ggtatcgcgg tggcgcaccg cggtttattg cgcctgatac aaggcgacag cccgctgaag
     2101 gtggagagcg gtgagacaac gctgctgacc tgcccatttg agtttgatgt ttcggtgttt
     2161 gagatgtggt ctaccttgct caaccacggc aaactagtat tactcagcaa acaggcatta
     2221 cttgatatca atcacattcg ccgcacgatc gctgatgaac aggtggcgcg cgcctggttt
     2281 acctcatcct tgtttaactc ctatgtggca gaaggtgccg atttcttcgg tatgttacaa
     2341 cacattacgg tgggcggcga agcagtatct gcgtggcatg tcaacgacgt gatgcaaaaa
     2401 tacccgcatc tggtggtgac aaacggttat gggccgacgg aaaacactat ttttaccacc
     2461 gcatatcgtt tcaacgggtt gcaacccgcc cgagtcccga taggatacgc ggtaccgggc
     2521 acctcgctct acattaccga tctccatggg catttgttgc ctatcggtgc caccggtgaa
     2581 ctagtggcgg gtggagtggg ggtcgccatc ggctatcaga acaacccggc gctaagtgcg
     2641 acggtttttg tccctgatcc ttttattccc ggcggtatga tgtacaaaac tggcgattat
     2701 gcccggttgc tggatgatgg ctgtgttgac tgttttgggc gtaaagacgg tcaaattaag
     2761 atcaatggac aacgaattga aaccggagag atcgagcagc gcctgctgga gtgctccggc
     2821 attatcgagg cggtagtggt tccttaccgc gtacgtgaaa cgctgcatat tgcggcagtg
     2881 gtctgcgtca atgatagcta tgatgaggtg gaagttcgag ggcaattggc tgacagattg
     2941 ccgccattcg ctattccgga atccttggtg gtggtgacgg agattgcaaa aagccatagt
     3001 ggcaaggccg acttggcgca gttgcggtat ctcctgcccg caactcagtg caacgccgtg
     3061 tccaccacga tatcagaggt gcatagtgac atggaacatg cgctgcatgc tatctggcaa
     3121 cgcgtacttg atagacaaga cattgatagc aatgcctcct ttttcgccct cggtggcacc
     3181 tcattggata ccatcagggt taaaggggat attaagcggc aacttggctt ggagattgat
     3241 attaccgatc tctttaagta cccaacgctc acggcgttag cacattttct cgatactgcc
     3301 gtatcgccgg aggatgcaat tccaacgcgt gctgttgtct acagcgacat gccggtggcg
     3361 attgtcggta tggcgggacg tttccccggt gcggcgaata ttgcagcgtt gtggacgctg
     3421 gttgtaggag gggaatcggg attaacactg ttcagtgatg aagagttgcg cgcgcatggt
     3481 gtgacacctg acacgcttaa acaagcgaat tatataaaaa ccaaagggat tgttgatgat
     3541 cacgaatggt ttgatgcgga tttctttggt tatactccca acgaggcgga atgtatggat
     3601 ccgcaaattc gcttattgca tcagtgctgc tggcaaactc tggaacacgc tgggtgcgat
     3661 cctgccacct ttactggtgc gattggcatt tatgccggac tgctgacatc cccccactgg
     3721 cttaatgcgg taatgcaaga cactaccgac tctgccgccc tgtacaaggc cagtatcctg
     3781 aatatccatt ccgtcacagc attgattgcc catgcgctta acctcaccgg ccctgccgtg
     3841 acgcttgaca ccgcctgctc cacttcggca gtggctatcc accaggcctg catcgcacta
     3901 cgtaaccggg attgcgatgc ggcattggcg ggcggcgttt ctatcgagat gcctgcgtac
     3961 cggggctatg aatatcatga aggcatgatc aatgcgcgag atggtgtctg tcgtcctttc
     4021 gatagccaag cctctggcac agtcaccggt gatggcttag gaatgctgct gctaaaacgt
     4081 ctggacgacg cgctggcgga tcgggactgc atctatggtg tgatcaaagg ttcggcggtc
     4141 aataatgatg gcaataacaa aatcggctat accgcgccga gtgtgatcgg gcaatcaacg
     4201 gtaatccgca ccagtttgcg ccgtgccggt tttgatagcg acagtatcgg attggtggaa
     4261 gcgcacggca ccggtacggt gctgggtgat cctattgagt tacgtgcgct taatgaggtg
     4321 tttggcccaa cacctgttcc gttttgtgtg gtttcagcgc tgaaaagtaa tattggccac
     4381 ctcaactccg ctgcgggagt ggcgggcgtc atcaaaacaa cgctcgccct gcatcatcaa
     4441 gtgttgccac caacagcgca ctttcgccaa ctcaatcctg ctatagatct atcgcgttct
     4501 gcattgtacg ttaaccagca agtccaaccg tggccgtcga cgcgtcctcg tcgtgcactg
     4561 gtgagctcct tcggcatcgg cggcaccaat gccagcatcg cactagaagc acatcaacat
     4621 gaggacgacc cttcagcgac gggggtacgc gacagctatc tgttgttgtt ctccgctaaa
     4681 acacccgctg cgttagagtt gcgcgtggcc tccacactgg aatatgtcaa gcatggagta
     4741 ggggtgcgcc tgccggatgt ggcttataca ttgcaaactg gacgcacagc ctttgaccat
     4801 cggcgggctt atttggtgag tcgtgggtcg aaaatcgatc tctcctgtgc cacgatattg
     4861 caagcggaaa tcttcaatgg tcagcgcacg acagcggaga tctgcttcat gtttcctggt
     4921 caaggtagcc aatatcacgg catggccagc gcgctctatg ctcatcaacc catgtttcgc
     4981 cagcacatgg atcgctgctt tgctgcattc caacgctatt cgacggtcga tctcaaggcg
     5041 ttgttgtttg acgatgagga tacgcgggat attgatcaaa cgcagttcac acaaccggcg
     5101 ttgttctgtg tcgaatacag cctagcgcgc accttgattg atctggggat tacgccggac
     5161 agtatgatcg ggcacagtct gggcgagtat gttgcggcct gtattgctgg cgtatttact
     5221 cttgaggatg cgctgcacgt cattgaggcg cgcggacgtt tgatgcagtc catgcgtccc
     5281 ggtagcatga tggcggtcta ccttagtcgc gaacagttga ccccatggct agctgcagaa
     5341 cggggtattg aactggcagc taataatagc gcgcattttt gcgtagtcgc gggcgagcag
     5401 gcggccattt cccgtttgag cacacgctta gtcgagggcg ggatacagca caggcgcctg
     5461 aaaacctctc acgcattcca ctcggccatg atgacgccga tgctgcacga ttttgcacag
     5521 ttgctggggc aaatcccgat gcacgcgccg cacaagcgct ttatatctaa tgtaagcggt
     5581 acatggatta ctgaggagca agctacctcg ccggattact gggtgcagca agtgcgcaac
     5641 gcggtgctgt ttagcgaagg tgcggcgcaa ctgttggtac aacccacgct gtttatcgaa
     5701 tgtgggccgg gtaatacgct ctctaccttt attcaaggac ataaccaata cagcgatcag
     5761 ccgaccctgt tgacgctacg caaagccaac gcggcgatcg atgatgagca catgttgcat
     5821 cgtacgctgg cggcgctgtg ggtcaggggg gagaatattg attggagacg ctttaaccag
     5881 acggcactcg gcaagcacat tccattgccg gattacccct tcgaacagac ttattactat
     5941 cgctatggtg ctgcactttc cggttatcgc cagtatccaa atcctctgcg ccgtccgcaa
     6001 gatgagtggc tccagcgtgt gctgtggcgg atgcacgaca catccttgcg ggaggcgttc
     6061 tatgcgccgg gcgaattgat catcattatt agtgctgacg gcgacaagtt acagcagacg
     6121 ctgatgagta gtggtgtcga cagcatcaca atgccgctgc cgatatcgtc agaggacgac
     6181 gtgtgggata atgaccgtat tctgacgcat ttccacgaca tctgcgcact tttagcacac
     6241 aaaacctacc gacagttaca ttgcttgtat gctcccggtg cggaggcagg atcatcgttg
     6301 acacagtcgc tctcgggact ttatcgtgtt gctcgctggt gtatgcacag caccacgccg
     6361 ctggcgtcct taacagtatt gacccatggt gcgtttcgcg tacaggaaga ggataatccc
     6421 gaaccgacgc tggcagcgtt gtcgggcgca gtaaacgtgt ttgcccaaga gctgcacccg
     6481 accgaagtac gattgatcga tatcgatgcg cagagcagtg atgagaatct gaacttgcta
     6541 acccagcgtc tggccccgaa acaagaaacg gtaatggcgc tcaggcaggg gatgctttac
     6601 ctgcgacgct ttatcccgac acggctgttg gcgcacttac cccctcaaac agggtgtata
     6661 ccgggcaacg tactgtggat tatcggcggt gaaaagggga ttggccgcat gatcggcgaa
     6721 gcgcttgctc agcgtgaggg agtccgtgtg gtactgagca gccgcacagg ttatcaccat
     6781 gaagcggtgc agcaggacgc attagacgtt atccactgcg acgtgacgca ggcggaggcg
     6841 gttagagctt gtctggcaac tctgctcgaa cgctatggac ggttggatgg cgtgattttt
     6901 gctgccgacg ctaccaccac attgacactg catcaattga gcgaatctgc gctacgcgac
     6961 acgctaacgg tgaaagaacg gggtactgct aatgtgctgc atgcgttagc gcaacggaat
     7021 ctgctggatg agcgtctgct actgctgttc tgtaactcgt tggctgccgt gaatgcggag
     7081 attggccaga caggctatgc taccgccagc gcctatttgg atgcactggc acagcaactg
     7141 cgtacccgct acaaggtgaa tgcgctcagt atcgggttgg atgcattgcg tgagcagggc
     7201 atgttgttgg atgctataaa cggcagtgaa tacgatgtct tgcgtggact gcgcccattg
     7261 atgacgggaa cgttgctaca ggcttataaa caacaagggg ctgacaccag ctactacgcg
     7321 cgattatccc ccgagtccga ttggctgctg gatgagcacc ggatatccgg tatcgccacg
     7381 ctgccgggaa ccggctatct ggcgctggcg tatgaagctc tgcgccatta ctttgtgcaa
     7441 gaccaaatct gcattgatga attggtcttt ttggcaccgt tgactgtgat ggacaactgc
     7501 agtgttgacg tttttgttga catttcacct aacgggcaag gagttagtgt cgaggtgaaa
     7561 tcaatgacgg agcgctttag tggcacgtta acaacccatg ccagaggcag ggcgacgcgt
     7621 ctgatggtag acgataatgt tgtgtgcgat ctcacggggc tgatgcgcga gatgcacact
     7681 atcactcctc caacaaagga attatcgagc acgcacttcc actatgggcc gcgctggcac
     7741 agcgtacaac aactgtatgg caataccgcc cagactcagg ttttcgcaac gctggccctg
     7801 cccaccgttg ccgctaatga tacgatcgca ctgcaccctg cgctgttgga catcgccagc
     7861 agtgttgccg aacaactgcc tggttttcat actgattcgg tacctttcct ttatcaggat
     7921 ttacgcctgt accgcccgtt gccgaacacc ttacatgtgg cgctgactgt caatcggcac
     7981 gatgaggagg gtgacagcta cgctttcacg ctctacgaca tggcaggcga gatggttgcc
     8041 cgctgtgcgg caatggtgaa gcgcaaggta cagctccaca tacaagatgt cgatgacgac
     8101 acgcgactgc gcgtgcccag tgccgataac taccaactgc ggctggccgc tgagggggag
     8161 ggggcaggaa agctagcgtt gtgccctacg ccgcgcttag cgctggggga ttcacaagta
     8221 gagattgagg tactggccac cggactgaac tttaaggatg tgctgttcac cacgggattg
     8281 ctccggcagc agccgggtga ggctccgctg caattgggac tggagtgcgc cggacgcatt
     8341 actcgcgtgg gtaaaaatgt cactgaattt gccccgggag aggatgttat ggcggtgcta
     8401 aacggtggtt ttgtccagta cgcacgggta gaaagcgatt gcgtagtgag aaaaccagcc
     8461 cattgtcgca tcgaacaggc ggctgcgctg cctatcgcat acctcaccgc ctattacgca
     8521 ctggtggtgc gcgctaattt gcaacccgga gaacgagtat tgatccacag tgcggcgggg
     8581 ggcgttggct tggctgcgct acatattgcc aaacgctgcg gagcacagat tttcgccaca
     8641 gcaggtagcg agcagaaacg cgattacttg ctttcgctag gcgtacatgc cgtagctgat
     8701 tcacacgacg aacagttcgc tgccactctg ctgaccgcat cggacggaca ggggatggat
     8761 gtgatcctta actccctcac aggccgtctg cttgacgcta gcctcgcgct gctggcaccg
     8821 ctgggccgtt ttcttgagct gggcagcaag gacattgtgg aagacaaagc gctaccgatg
     8881 cgtttcttcg cccaaggcgg cacctttatt ccgattaatt ttcacgcggc gcatggtgcg
     8941 tttagtcgct acctgcaaca gattgtcgct tggatagatg ataacacgct accgctcctt
     9001 ccatgcaaat ccgtaccatt gcccgaggtt gcacgcgcct tcgccaccct gaccacgccg
     9061 cagcatattg gcaaggtggt ggtaacgcat cgcactgcgg caggcatgga ccggctgaac
     9121 gccatgatag cagaaaggcg cctcggcggc tatgcgctca gcatgagcaa tgccgaggtg
     9181 atgcgccaat tgtggccgat actgaacact cgcagtccgt gggcgcaact gctgctctca
     9241 cctcgggcga tcgatagatt agcgcggggc aaccgggtgg atcgcggtgt accgtctgcc
     9301 gctaacgata cgattactca gcagacagtg aaaaagcggc cccgtccgga aattggcgtg
     9361 ccttacagcc ccgcgacacg tgaagtggaa cgcgtgctct gccaaatcct ggaagagtat
     9421 ctgggtctgg atagagtagg cattgacgac aactatgccg aattaggggc aacctcactc
     9481 gatatggtgc aactgagtgg gcaaatggcg cgtcactatc cgcaagtgag cgtggtgtcg
     9541 ctgtacaacc acgccaccgt gcgccagctg gcgacgtttt gccagccccc ggagggcgag
     9601 tcaaatgcgc catcaccaca gcctgcagta cagacgaata cgcgcgcgaa tcagatagca
     9661 aaacgtgctt tgcaaattgc gaaaaatact gcgcgcagtc acacgtcttt gcattaatcc
     9721 gggcgcctcc tcgccctgaa gaaaggtaat gaagtttatg gaatacgcaa gcgaaatgaa
     9781 cggcatggaa atcgccatta ttggtatggc ggtccgtttc ccgcagtccc ggacgttaca
     9841 cgaattctgg cataacatcg ttcagggtaa agagtgcgtc accttcttta gcgaggagga
     9901 gttgctggcc gaaggcgtcg aacagagtac tctggacaac ccggcctatg tacgggccaa
     9961 gccctatatc gagggcatat gcgactttga tgctgcattt ttcggttaca gtcacaaaga
    10021 ggcgcagact ctggatccga aatcccgcgt attacatgaa gttgcctacc atgcgctgga
    10081 agatgcaggc tatgcccaac gtaccagcga tctgatcacc ggggtattcg tgggggcgtc
    10141 agaagatgtg gattggctac ggcgttcgct gtcacagatt ggcggcgatg cgctgaatcg
    10201 ttttgagtct ggcatctatg gtcataagga tctgctcgca catctcattg cctacagtct
    10261 caatctcaac ggtccggtgt atagtctcta caccagttgt tcgacttctc tgagtgcaac
    10321 gcatatcgct tgccgcagct tgttgtttgg cgaatgtgat ctggcgttgg cgggtggaat
    10381 tactatcgat ttaccgcaga agtcaggcta cttctgtcaa cagggcatga tccactccac
    10441 cgatggccac tgccgtcctt ttgacagtca ggcttctggc accctgtttg gcgatggcgc
    10501 gggtgtggtt gtgcttcggc gtttggaaga tgctctggca gcgggcgatc gcatctatgc
    10561 ggtgatccgg ggtagcgcgg tcaacaatga cggtaaacaa aaaatcggtt ttgtcgcgcc
    10621 tggtcatgag ggacagaagg cggtcatttg tgcggcctgt catctggcag aagtaagccc
    10681 agaaagcatc ggctacgttg aaacccacgg taccggcacc cgtattggcg atcctatcga
    10741 gttcgctgcg ttgacggagg cgttcgatac ttcacaccgc caatactgtg cactaggggc
    10801 tgtaaaggcg aatattggcc acacccacgc ggcggcgggc gtggctgggt taatcaagac
    10861 ggcgctggta cttcatcacc ggaccattcc gccgctcgcc aactatcaaa tgcctaactc
    10921 gaagctggat ctggcgcatt caccgtttta tatcccgata cagccgcagg agtggcccgc
    10981 atcgcggatg ccgccccgcg ctggcgtcag ttcttttggc attggcggta ctaatgtgca
    11041 tatgattttg gaagggctga atcctgcggt gcgcgatgac catgaccaag tgcgagcacc
    11101 ggtgtttatc cctctctccg cgccgtcttt cgagcagttg gatgagctga cgcaacagct
    11161 taccccgttg ctggctaccc ttgatgcgtc aacgctagcc tacacacaac aagtggcgcg
    11221 ccccgtgttt gattgccgcc gagtgataca agtggaaaac gatggtacgc aagcgatgct
    11281 ggcatcgctg gataacctaa tgcccgacgc tccttggggc ctacactgtc cagatctacg
    11341 tactacgaac gattgtactt acgcacagtg gctggcgcat tcggcacatt atcaacgcga
    11401 agcgactgct ctgacggcat tactcgacgg catgaatatt ccacccgctt attgccacgc
    11461 tgaaacgtgg gcggcacaag cgaacagcag cctgctaatc agaggctgcc agactatcgc
    11521 cgcgctaaaa acctggatga acctgctacc gacattaaca ctgctgtcgg gtgctggaac
    11581 aggccttttg cctgccgctg cagccagtgg catgattgcg acgcaagacg tgttgcattt
    11641 gctgtgggaa atggagcaaa aggcgcttca tctctggctt cctgagcgcc atgaacctat
    11701 ccccggctac gtgctggctt ggcaaggaaa tcccatcacc gatgcacagc gtaacgacag
    11761 agggttttgg agtgaagcgc tgttggcaga tacccgagaa ctgggggagg gcgttcacag
    11821 tatcaactgg gttaggctgc cgccggaaat aagagaagac gttgatgtat tgcgctacgt
    11881 ggcgcaactg tggtgtgcag gtatcaatgt ggattgggcc gtgtggtacg gcactccgct
    11941 gccgcaacgc gggagcgcat cagcatatcc cttcgcacac aaccactacc cattgcctgg
    12001 acgggtaatg ggtagtgtgg agacccaacc cgaagcagga cccgaaacgc accaccctta
    12061 tcaggcacgt cccgtgttaa gcgtaccttt cgtagccgca cactcgcggg gtatgcagta
    12121 tatcacaggt ctgatggaac tgttgctgga aatatcgccg gtcggggtgg acgacgactt
    12181 ttttgaatta ggcgggcatt cgctgctggt gacgcaattg acctcccgtt tagaacgcga
    12241 tttcaacgta catatcgatc ttttgaccct gatggaaaac cccaatccgc gcaatatcta
    12301 cgcgcatatc gcggcgcaac tggggggcga agacaacctc gaaatagcct gtcagtaaag
    12361 gagctcaatg atgaacgtgg cggtaatagg tgcaggagta atgggaactg gcgtcgctca
    12421 taacatggcg caatacggca tatcaactaa cgttgtggat atttctcaat ctcagttgga
    12481 taaatgccga cagatgatcg aagcgaactt acgcttatat aattttcacc cgcagcataa
    12541 aaaaaagacg catagcacag cggagataat ggaaaatatc cgtttcacaa ctgagttgga
    12601 cgacattgta gaatgtgatt tggtgattga gaatattact gaggatatag agaaaaaaaa
    12661 tgcactatac acacggatga atacgatctg cggcgcgtcg acagtgtttg gtgttaatac
    12721 gtccgccata tctatcactg cgttgtcaaa attaatgcga catccggaga atgtagtcgg
    12781 cgtccatttt atgaacccgg taccgctgat gcacaccgtc gaattaatcc gtggtgtaca
    12841 tactgcagag cgtacactga acatatttca ccacttattt gctcagttga ataaaaccgg
    12901 catcgtggtc aatgattctc cgggctttgt cactaatcgt gccatgatga tttttgttaa
    12961 cgaagccata tttatggtgc aggagcagat cgcccgtgct gaggatatcg atacgttgtt
    13021 taaaacctgc ttcgggcaca agatggggcc actacaaact gccgatttga ttggcttgga
    13081 tacgattttg caatcgttgc aggtgctata tgaaagtttt aatgatgata agtatcgccc
    13141 cagcttttta ttaaagaaaa tggtcgatgc agggtatttg ggcgtgaaat cagggcaggg
    13201 gttttatcgc tatcaacaga cgtacgccga gcagtgattc tgggttaacc agatagggat
    13261 atgaatatga aaaagcaaga tatgaaagcc gccattcggg aatttctttc acgctcatta
    13321 cgtgggcata cgttgaacga tgatgacgat attttttctc tcgggcttgt ccattcgtta
    13381 tttactgtgc aaatcatact gtttatagaa aaaaattttc aggttgagct ggaagtgagt
    13441 gagttgaaaa cagaacagat tgctaccgtc aataaaatag tggagctcat tcagcgacaa
    13501 acaggcctgg agtaaactat gtgcactgaa aattatgagc tggctcagca agaggcagtc
    13561 ctgtttgcca aacaacatct cgccctggct gcacagaata ttgaacgtca gcagtttatt
    13621 gtgccagaca ttatttcttg cgtggcgcag gccgggtact taggtgcgtc aatcccccaa
    13681 aaatatggcg gacgaggtta cgattcttat caactgtgcg ctttgcatga agtgatggct
    13741 ggggttcacg gttcgttaga aaatctcata acagtgactg gcatggtcag cacgctgctg
    13801 caacgcgtgg gcagcgcagc gcaaaaagcc cattatttgc caaagctggc aacgggggaa
    13861 ttgatcggcg cgattgccct cacagagccg aatatcggca gtgacttagt gaacgtggaa
    13921 acagaattac agcaggatgg cgacggttgg cggctgaacg ggaaaaagaa atggatcacg
    13981 ctagggcaga ttgctgattt ttttattgtt ttaatccact gtggtaatca attagcgaca
    14041 gtgcttatcg atcgcaatac cgacggcttt actatcactc cactcaacga catgttgggg
    14101 ttacgcggca atatgttggc agagctgcat tttaatgact gccgcctgaa ggaggatgca
    14161 ttgctcggtc cgctcacccc tggggtaccg ctggcggtga attttgcgct caatgaaggg
    14221 cggttcacca ccgcttgtgg cagcctagga ttatgtcggg ccgctgtgga tgtggcagcg
    14281 cgctacatcc gccagcgtaa acagttcaag cggcggttat tttctcatgg cattgttcag
    14341 cacttgtttg ccacaatgct gacccaaacg cgcagtgcgc aactaatgtg cttcagcgcg
    14401 gcggaatacc gcgaaacgct gcacccggcc atgataaacc aaatcctgat ggcgaagtat
    14461 gtcgcttcca aagctgcggt ggacgtggcc gggaaggcgg tgcaactgct cggtgcgaat
    14521 ggttgccatg ccgattatgc cgtcgaacgt tactaccgcg acgccaaaat catggaaatc
    14581 atcgaaggga catcgcaaat tcatgaaatc caaattgcga tgaattacat gatggggagt
    14641 gaagcatgac gaaggatgtc gcactgatgt tccctggctc cggttcgcaa tatgtaggca
    14701 tggcacggtg gctgtatgag cgttatccgc aggtgcgtac tctgtttgat gaagctagcc
    14761 agatcacgga acgggacatg gcagcgctgt gcctgtcggg tactttggta caacttgctg
    14821 agccgacggc aatggcgctg gctatctata ccaccagcgt ggcccacttt gtcgcgtggc
    14881 agcagttttt ggcgcaaacc ggtgtccatg tcaacctacg ttatatgttg ggtcatagtt
    14941 tgggcgaata tgctgcgctg acctgtagtg gtgcgctgag cttttcccaa gcactggcgc
    15001 tggtggcgat gcgtagccgt ttagccagtg agatcgcccg cgaaatggac gcgagtacca
    15061 ctatcatcaa gcaagggaat caggcactgg tggcagcggc gtgcgaggtg gcggagcgtg
    15121 aaacccgaca gcaggtcggt attgcctgct tcaattcgcc gcaacagttt atgttatccg
    15181 gacaaaactc ggccattatc gccgccgagc aatacctgct ggatcatgat cgccaagtcg
    15241 aagtagtgcc gctgattggt ggtgttcctt accacagccc actattgaaa ccttgcggac
    15301 agcaattgcg caaggcgctg gatcgctgcg aatggcgacg tccgtgctgc ccagtgatct
    15361 ccaatgtcaa cgctcaaccc tatcccgata cggtcacgcc agtccagtgg ctagaacaac
    15421 agctctctca gccagtgcag tggcagcgct ctctcaccta cctgacagga cacttgtcac
    15481 cgatcgcgat agagatcggt ccgcaaagtg tgctgaaaaa cctgctgctg gagaatcgct
    15541 atccagcacc agtttacgca tttgacaatc gtcatgatcg tgcgcaacta gcattggtgc
    15601 tgggggataa catggcggtg aagaccgatc cggaagccgt gcgccgccaa cgcatcacgc
    15661 tgctcactaa cgccctgaca gccacacgcc atcaccgcgc cgccgatgtg gctgccagtg
    15721 cgcaattgaa agaattgctc agtcgttttt ttgagcgcat ccagcagatt gagcagcgcg
    15781 gcacatccag cgaagaggac attgcttttt tgcatgagct tcttgaacag ggatttcaac
    15841 tcaaaggcag tagtcaggca gaaatcgacg cctgccacgc gcggttggct tccgacaacg
    15901 gcggacaggc gtaacccgtc acgccatttt acgtacttat ctcgctttga aggccagagt
    15961 tatggaacag caagggatta tgagacagtt gcctaccgac gaccaaacga tagtcgacta
    16021 tttgtatcgt atcgccggag aatatgggga aaaagccgct gtattgatgg gggacgcggc
    16081 gctgagctat cacgatctta atgcacgctc taaccaactg gcgcattatc tgcgtgggct
    16141 ggggatcggc gaggatcgtg tggtagctat ccgcctgccg cgcggcatgg caatgctgat
    16201 tgccattttc gctattgtaa aagctggtgg tgcctattta ccgctggcgt acaatgcacc
    16261 gcgcagccgc attgaaaata tactgagcaa cagcggcgct gtttgtctga tcggtactga
    16321 cgatggtgat cgctggccga ttcctcgcgt cgaaatcgac agcgcggcgg tcagtgccat
    16381 gccaacgacg gatctgcgtt accgaccaca tgcgcggcaa ctggcgtata ttatttacac
    16441 ctccggttcc accggtgtcc ctaaaggggt ggcgacggaa catgcagcgc tgctaaaccg
    16501 aattgtctgg atgcagaacg cttatcctat cagttctcag gacgtgctgt ttcagaagac
    16561 ggtgtacacc tttgacgtct ctgtctggga gatgttctgg tgggcgatgt acggcgcatc
    16621 tgtagtgctg ttaccgtccg gactggagag cgatccgcga accttggctc gactgattca
    16681 gcgtcaccgc gtgtcggttg tgcactttgt cccttcgatg ctgaacctgt ttgtcgagta
    16741 tctggagatg aaacaggatc ctcgtttgac cgcctcattg cgattagtgt tttccagcgg
    16801 tgagaaactc acggtccaca gtgtggctcg cttttatcaa tcggtggcgc agggtgatct
    16861 tataaatctc tatggcccga ccgaagcagc aatcgatgtc agtcatcacc gctgcctgcg
    16921 cgggtacgac tacgacgata tccctatcgg tcaagcgatt gacggttgcc gactctatgt
    16981 gctggatgac catggtaatc cggtagcaga cggcgaagag ggcgaactgt atctcgctgg
    17041 tatcgggctg gcacgtggct atctcaacaa cgtggcgtta actgatcgct gttttactat
    17101 acatccaact ttgcgccatt tagggaaacc ggagcggctg tataaaactg gagatctggt
    17161 gtggcgcgac ggggaaagcc aacaaattca ttacattggc cgtaatgatt ttcagattaa
    17221 aattagaggg ttgcgcgttg aattgggaga aatcgaagcc catgcgatgc gtttcccggg
    17281 ggtacagcag gcagtcgtgg tggcggatca ggatgatccc gacaatcagt tgatttacgc
    17341 ttttgtcgtc agtagcgtgc cgctcaattt ggcggcctta atggacgcac tgtccaaaaa
    17401 cttgcctgcc tacatgctgc cgaaccgttt gttggcaatg tcagagttac cactctccga
    17461 caatggcaag tgctgtcgta aaacgttgct cgacttggcg cgggcgtact cagccagtcg
    17521 cgtcgattta cgtgaaactc ccgccgtgcg ctacctaccg ttgtcatcgg ctcaatcgtc
    17581 gatgtggttt atgcaacaat tggcgccgca tactgcacta tacaataacc ccaccgcctt
    17641 gctgcttgaa ggagaactgg atcgcacgcg gatggacggc gcgattcgtc aattgatgag
    17701 tcggcatact ctgttacgtg ctatggcgga aacccacaat ggacaaccag tattggcggt
    17761 gcctcagtgc gtatcgtcgc aggcgttgct gaccatagtg ccactcccct cggtgagtga
    17821 tgataacgcg ctacaggcga tgatcaacca gcgtgcggca caccccatgc ccttaacgtc
    17881 aggcacaccg ctgtgccggt ttgaactgtt gacgatcgat gacgatcgta gcgtattgtt
    17941 gattcatctg caccacatca tcagtgatgg ctggtcgaaa ggcgttttgt tacgcgaatt
    18001 gcaggccgca tataacggtg aatcgctgac gccagaaccg ttgttggagt atgccgacta
    18061 catggagtac caagaagagt ggcgtcagag tgacgcctat caggacgcga tgcgttactg
    18121 gcaaaatacc ctggcgggga cgttgccgat tcttgatatc cccaccgatc aaccgcgtca
    18181 gaaggtggca cgttaccaag gcgcgtttgt tgccttcgca ttgtctgcca acacatgcga
    18241 gcgagtgttg gcggcggcgc gtgcgcagcg cgtgtcgttg tataactatc tgctgaccgc
    18301 cttcgtcctc ctgctgcatc gtaatgcgcg tcaacaggaa tacatcgtcg gtatgccgat
    18361 tgctgcgcgg ctgacgaaag aacaggagca tatgatcgcg ccgctggtca acgtactacc
    18421 gctgcgctta cctcttgacg aagcggcatc gttttccgag ttagtacaga cgatcagagg
    18481 tattctgttt gccgctttca ggcaccagcg tctcgaattt accgacatag tgcgcgcagt
    18541 gaatgtggat cgcagcgccg gacatttccc aatatatcag tgcatgttcc aactcgacaa
    18601 tatgcctctg gcgagcccga cactaaacgg tgtcaacgtt acaccattat tgttggatac
    18661 cagtgcatct caggtggata tttcgctaag tatgcagcat atcgatgggc gcatcaccgg
    18721 cactttcgaa tacgacgctg ggttatacag tgcggatcgt attcagcacc tggtggcgca
    18781 gtggagagaa ctgctagatg aggccagcag ccaacccacg cagttagtcc gcgatttgat
    18841 tcgtttcacc cctcgagagc acgcttggct ggcgcggcac aacgccactg aagttgctct
    18901 gccgcccgta gataacctgc tggcgctagt gttaccgcac tgccagcagc gccctacaca
    18961 ggtggctttg cgccacgctg acgacgccat gacctatggc gaattgcaac aggccacgat
    19021 gcagatgtgt acttggctgc gtgcgcaagg cgtaaaacgc ggagaatctg tcgcactgca
    19081 actgcctttt tgtttcgaat tgattatcgc acaattggcg atcctctctt tgggggcgag
    19141 ctatgtgccg ctggatggta acgcgccagc ggcccgcaat gccctgatct tggcgcaggc
    19201 aacgccgtgc atgctgctgg tggcgcaacc gctggaatcc cctcatgggc tgacgatacc
    19261 ttgggtgcta gtgcctgact ggcgtagcct gctgacagaa ataccgaacc tgccagttag
    19321 tgttgcgcca gatgctttag attgcgatgc ggtggtgatc tttacctccg gtactaccgg
    19381 gcagcccaaa ggcgtccgac tcagccaacg taatctggtc aacttaaccg catcctttat
    19441 atccagctat caagtgacgc atcaggatgt tctgctaccc attacctcag tcgcgtctgc
    19501 tagctttgtc ggggaggtac tgccgctgct cgccgctggc ggtaccttag tattggcgca
    19561 aaaagcgcag agtttggata gtgatgcgct cattgcgctg ctggcatctc agcgggtgac
    19621 tatcctgagc accaccccat cgctttctgc cagcctctct gtgctggctc agtcgatggg
    19681 atcgctgcgc ttgtttctgt gcggtggtga agcgttagag tatgagcaga tagcgccgct
    19741 tctgccgcac atggcagtgg ttaacggtta tggcctgacc gaaagcggta tctgctcgac
    19801 ctactttcct gtcgcaaagc gtagggagca agaaacggga gcattgccca ttggccgccc
    19861 gattcagaac acccaagctt atgtggtgga tgcatataat cgtttggtac cgccaggtgc
    19921 ctgtggagaa ctctgtttct ctggtctggg catttcgccc ggctaccttg atgcacgaca
    19981 ggatcccgag cgctttgtcg agttgccgga ataccctggc gttcgggtgc tgaaaactgg
    20041 cgatcgtgct cgttgggcaa ccgacgggat gttgttctat ctcggcagac aagatcgcca
    20101 agtgcagatc cgtggatacc gagttgaact gggcgacatc gaaagcctac tgaaacagca
    20161 tccggatatt gctgatgcgt gggtcgatgt gcgacgcaat gcggcggcaa cgccgctact
    20221 agtggccttc tattgcagcg tcaacggcgt tgcgctggat gctcagcaat tgcgcgtatg
    20281 gctcagcctg cggcttccgt tgcatatgct gccgttgctc tacgtgccgc tgagcgccat
    20341 gccgctaggt gtaaacggca aaatcgatcc ccagtgcctg ccgctggtcg atctgcggca
    20401 gttggaaggg ccgggcgagt atgtccctcc ggcaaccgaa ctggaacagc gtctggcgga
    20461 gatctggcag cagttgcttg gcctggagcg tgtcggcacc accacaaatt tcttcgatct
    20521 cggtggacac tcacttctgc tagtacagat gcagcaatac atcgggcagc agtgcggtca
    20581 gcacgtggcg ttggttgacc tgctgcgctt cactaccatc aaacgcttgg cggaatttct
    20641 gctggccccg gacgcagcgc aagggaccac aggagatcag acacaactgc gtgcggcgaa
    20701 gcagcgtttg gcctttggtc acacgcgctg ggcagccaca accgacagtc atcactgatt
    20761 ccactgaatg aaacgcgtga taaacctgaa tataggggga aagcaacatg gcagagaatg
    20821 attttggtat agctatcatt gggatggcgg ggcgtttccc tcaagccgat acggtacagg
    20881 cgttttggga aaatcttctg gcaagccgag agtgcatttc cttttacagc gatgaggaac
    20941 tgctggcgat gggaatcagt cctgaatttg ttcagcaccc agattacgtc aaggccaaag
    21001 gggaggtagc tgacatcgat aaatttgatg ctgctttctt cggtatcgca ccccgagagg
    21061 cggagctgat ggatcctcag caccgtgtcc tgttggaaac cgcgtgggca gccttcgaag
    21121 atgcgggcta tgtggctgcc gattatccgg gggatgtggg tatttttgct ggcaaaagca
    21181 tggattccta tttgatgctg aacttgatgc cgcactttaa acgcgttttc tcttctggca
    21241 gtttgcaggc ggccattggt aacgacaaag acagtatcac tactaccatc gcctatcacc
    21301 tcaacctgcg cggcccggcg atcacggtgc agacttcctc atcgacatcc ttggtggcgg
    21361 tgtgcgtggc atgccaaagt ctgttgactt ggcagtgtga catggcgatt gctggcggag
    21421 taacgctggg gccaccagca aaaaccggct acttgtcgca ggaaggtggg atcaccgccg
    21481 ccgatggcca ctgtcgggcg ttttccgata acagcagcgg atttgtgccc ggcaccggcg
    21541 cggggctggt ggtgttaaag cgtgttgatg aagcgctgcg tgatggcgat aacatctatg
    21601 cggtcatcaa aggatttgcc gtcaacaacg acggttcgga gaagatcagt tataccgcgc
    21661 cgagcgtgga tgcccaagcg cgcgccattg ctcaggcaca gcggttggca ggtttgacac
    21721 cgcaggatat cacttatgtg gaagcccacg gtaccggtac gcgtttgggc gatccggttg
    21781 agttttctgc gctaagccaa gcctttgcgg gcgcgtcaca aaagcagtac tgtgcgctgg
    21841 ggtcggtaaa aaccaatatc ggccatttgg atacggcagc gggcgtcgca gggttgataa
    21901 aaaccgcgct ggcagtgcag cagggaataa ttcccgcaac attgcacttt gaacggccca
    21961 atgcacagat cgatctgacc aatagtccgt tttacatcaa cactacctgt caaccgtggc
    22021 aaccggaaag cggcatccgt cgcgcgggcg tcacctcgct tggcatgggc ggcaccaatg
    22081 cccatgttgt gctggaacaa gcgccagccg ttgacttgca agcgcgagcg cctgtacctg
    22141 cctacagtat tttgccgttt tctgccaaga ccgacagtgc cttatcctcg gggctggcgc
    22201 gttttgccga ttttctgcaa catgaatcgc tgccggatag gcgggatctg gcgtggacac
    22261 tctcacaggg acgtaaagcc ttcgcacatc gcgccgcgct cgtaaccaga gatctacatg
    22321 ctgccgggac gctgttgcag caggccgcga cagcgccgtt tgctcgtggt gtagcgcaga
    22381 cacagctcgg gctggggctg ttgttttccg ggcagggtag ccaatatcag cgcatgggcc
    22441 atcagctcta tcaggtttgg ccggcctacg ccgatgcctt cgaccgttgc gcgacgttac
    22501 tcgagcgtga ataccaactg gatatccgcc atgagttgtt cagagcagaa gtctcgttag
    22561 cccaaggcga acgccttgcg caaacctgcc tcacacaacc gctgctgttc agcgtcgagt
    22621 atgccttagc ccaattgtgg ctcagttggg gaatcacgcc aacggtaatg attggtcatt
    22681 cgctgggcga atgggtggca gcgacgttgg caggcgtgtt ctctttagag gatgcgttgc
    22741 gcttggtggc gcgtcgagca gagctgatgc atcaggcacc aagcggtgcc atgttgatgg
    22801 tggcgctgcc cgaagcacag attcgcgcac tgattaccgc cccgctggcg attgccgctg
    22861 ttaacgcccc tgactattcg gttatcgccg ggcctacgtc ggagatcctc gccgtcagcc
    22921 agcgtctgac ggagcagaac atcatcaaca aacgattgca tacctctcat gccttccact
    22981 ctagcatgat gcaagatgcg gcgcaggcgc tacgtcaggc atttgagaat gtacgactga
    23041 acccgccgac gctcaccatc atctctaccg taaccggcgc gcacgtcagt gccgacactc
    23101 tcaccacacc ggactactgg attgaacaga tgctgatgcc tgtgcagttc tccgctgcac
    23161 tgcaagaggc gcaagctaca tttgatgtcg attttctgga aatcgggcca ggtgccaccc
    23221 tgacccagct gaccaacggt catgcattag gtgatcgtct cgcgttcagc tcgctgccag
    23281 caggtgcccg cagcagcgat gagcacaaac atatcttaga taccgtggca gcgctttggg
    23341 tgcgagggca taacatcgat ttgtctgcgt ttgcaggtga gcaaccgcgc cgtgtctctc
    23401 taccgaccta cgcctttgac aaaatccgtt actgggtcga cagtccagaa gaacagagga
    23461 gcgccgtaac gccggtggcg gacgcgggaa gtgtcatccc tagcgaaccg tcggtgcgcc
    23521 gtcagccgcg tccggcgttt tcggtgcctt acgcggctcc agagagcaaa acgcagcgcg
    23581 gcttagtggc gatttgtgaa gcattgctgg gaattgacgg cttgggcatt gacgacaact
    23641 tcttcgaagc tggcggacat tcattgatgc tgggcatgtt gctggcgcag gtacaggaac
    23701 ggttcgccgt cactctttcc tttttcgacg tgatggagga tgccagcgtg cgcgcgttag
    23761 cgcagttggt cgagcaggag cagcaggatg atggagggtc cgcacttgcc gtgctagtta
    23821 acgacatgat taatgagtga cgttggcaaa agtatttaac acagcgaaaa ggaaaggacg
    23881 gtcatgacga tacatcatgc cgcattggcg cgaatgttac cggcggaaaa aaaggaaaag
    23941 ctattacgac agttggcgca atcaggcgtg tcgcccagcc gcattcccat catcaaagcg
    24001 gatccggcgc aggcaatccc gctgtcgttt aatcaggagc ggttgtggtt tttgcaaaaa
    24061 tacgacagta ccgccactaa ctacaacctt tatgtggtgt atcgtttgca tggcgtggtg
    24121 gatatgccga tgctcacgga ggcattgcgc catgtgcaag cacgccacgc catcttgcgt
    24181 acccgtatca tagtacgtaa cgatcggcct tgccaggtga tagatgatgc gtcttcgtta
    24241 gttctcgata cggtcacgct ggcagcgcag gccccaactt cagcgctaga tgcggtaatt
    24301 cagcaggtga taaacacccg gttcgatctg gcacgcggtc ctctgtgggg ggtaactcag
    24361 attattcaac ccgatcaagg ttgtcatttg gtgttttgcg cgcaccatat catcatcgac
    24421 ggtatctcgt tacggctatt gttcgatgag ttacagcagc agtatgctcg attgcatgct
    24481 ggtaatgaaa cgtcgctgcc tccgccgccg ctgcaatatg ccgattatgc cttctggcag
    24541 cgcgaatggt tccaggatac cttgctggcg aacgaacttg cctactggcg tgcgcgtttg
    24601 caagacgcgc ctctgctgtc gacatttcca tcgctgcacc cgcgcccggc acaaccctct
    24661 acacatggtt cccggttcag catcaccctg gatgaaacat taagcctcgc gttgaaacac
    24721 gtagcccgca cgcaggaaac aacgccgttc gtgctgatgc tgactgcctt ccaactggtg
    24781 ctgatgcgtt acgctcagca acagcgattg gtgataggca tgccagtatc ggggcgcatt
    24841 cgcccggaat tgcagagtag cattggttat tatgccagca ctgcggttat ctacaccgat
    24901 tttaatgggg ttgaggtagg gcgcgaggcg ctccagcgtg tgaaggccag cgtgaaagag
    24961 acgcaggggc gccagcaact gccgtttgaa aacttggtga atatgctgga tctccctcgc
    25021 agcctttccc attcaccgtt gttccagatc ctctatatct accataacca cgtgacccca
    25081 cgcgctttta ccttggctgg tgcgtattgg gagcaggtga cgtatcacaa tcagaccgtc
    25141 aaatacgaca tgacagtcga ggtgttccaa aacgacgcca cgttcgacgt ctcctttgag
    25201 tacgatttgg ggttatacga tgctgatgtg gtgaagcaga ttgccgaggc gctgcgtcag
    25261 cactgcttat cgttgacatc atcactggag accccgatag gggcgatccc tctgcatgca
    25321 ccggagaccg caacgccgcg gcgtgatccg ctcaacgcca ctaacgtccc gtggctcggg
    25381 ccgcaggatg tgctgcgtat cattgaacag cgctgtgtgc agcacccaaa gcaactggca
    25441 atacagcagc atgacggcac actgacctac gctgagctct gggcgcgtgt gcagttcatc
    25501 gcgatgcgtt ttcgagcgca tggcatacaa ccgggcgatc gtattggcgt gctgttgcca
    25561 cgtcacaggg atgtgattgc aaccatgttg gcgacgtggt ttgtgggggc gtgctacgtg
    25621 ccgttcgata ttcatcagcc tgccgcgcgt ttgcaacgcc ttatgcaacg cgcgcgtttg
    25681 gtctgtctgg tggtccgtca gccgggagag tggggtgaaa ttgtacaact gtcgttgccg
    25741 gaattgatgc aggacatgtc gaatgcgatc cggtattcta caccttgcgc gctgttgccg
    25801 gatatgcagg cctacctgtt gtttacctcc ggcagtaccg gtgaacctaa aggcgtgtgc
    25861 gttgttcacc gcgggttgct gaacttgttg ttggatatgc agcgtacctt tgcggttggc
    25921 tcgcaagacc ggctgctctc ggtgacgacg ccaacatttg atatctcatt cttggagtat
    25981 ctgttgccgc tgatctccgg tgcgagtctc tatctgacag aggcggaacg cgccgcagac
    26041 agcttccgta tgattccgct gattgccgac tatcgaccaa cgctgatgca ggcgacgccc
    26101 tcgttctggc acgggctgtt gatggcgggt tggcgtggcg acccggaact atgtgtgctg
    26161 gcaggcggtg aagcgctgcc aacgaaagtg gcggaagaac tgttgcgctg ttgtggttca
    26221 ttgtggaacc tctacggtcc caccgaaaca accatttggt cgctgaaatc gcagataacc
    26281 caagcggaaa acatcaccct cggcgctccc attgccaata cccgtatata cattctggac
    26341 aatgagggcc atccagtgcc gcaaggcgtt gacggcgagc tttacatcgc cggggatggt
    26401 gtggcgcaag ggtatgatgg gcagcctgag ttgaacgcac agttcttctt gtcagaacca
    26461 ggggtccccg gtggcaggat gttccgcact ggcgatctgg tcaggagtga tgcgcagggg
    26521 cagttgtttt ttgttgggcg caaagatagc cagatcaaac tgcgcggtta tcgtattgag
    26581 ttaggcgaaa ttgaacggac gttggcacgg catccgcatg tggacgccgc ggtggtggcc
    26641 tgtattgaaa gagcaccgtt acacaaagca ctggcagcat tcatcatcac cagtgagcct
    26701 ccctcgctat tcgagcaact gaaaaacgag cttcggcagc aactgccaga ctatatggtg
    26761 ccaacgctgt ggcagagggt ggccgacttc ccgaacactg acaacggtaa aatcgatcgt
    26821 aagcggttag cggaaaattt cgttgccgat agctcccttg tgtcgccgca gacgcaagcg
    26881 ctgagcgaca cggaacagat gttgctggcg ctgtggatgc gctatttgcc gataaaaaac
    26941 gtcgatcctg agtgcgattt ctttcgcttg gggggacatt cgctgttggc ggtaacgttg
    27001 gtagcagaga tcaaccgcac ttttcactgc gccttgaccc tgaaggacat tttccactac
    27061 tccacactac gggcactgag tgcgcgtatt gcacagcaat ctatcacgga cgctgccgcg
    27121 tctcaggatg actgggtgat agtgcacgac cctgagcatc gtcatcaacc gttcccattg
    27181 acggatgttc agcgcgccta ctggcttgga cgacagacgg gtgctacctc gatcgcgacc
    27241 cacatctacc atgaatttga cgtagaacac tttaatgtta cgcgttttac ccatgcggtg
    27301 aatgcgctga tcgctcgcca tgaaatgcta cgtgcgcggg tactccccga cggtactcag
    27361 cagattctgg cgcaagtgcc ggcgtatcag ttagagcagc gcgatctgag tgctttgtcc
    27421 cctaacgcac gaaacgatgc cttgatggcg atccgcgatc ggctgtcgca tcatgtgcat
    27481 cccgcagatc gttggccgct gtttgatttc agttattcgg cttgcacggc gcaacatggc
    27541 cgcttgcatt tcagtctcga tctgctgatt gccgatgctc tgagtatgcg cacgctacag
    27601 caggagttga tgatgctgta ccgtgagccc catgtgtcac tgccgttgct accgttctct
    27661 tttcgtgact acgtgcaggc gctgttggta gagcaggcga gtgaagccta tgcacgcgat
    27721 caggcctatt ggcaacgggc gctgccgcag ctgtatggcc caccaacgct gcccgtacag
    27781 ggcgatttgg cgcaactgtc tgcgatcagg ttcgtacgtc gccgtcatcg gctgtcagcc
    27841 cacaactggg gagtgctgag cgcgctggcc caacgcacac gtatcaccaa gacggcattg
    27901 ttgttgacag tctttagcca agtgctggca cgttggagcc ttagcccgac gtttacgctc
    27961 aatctgacgt tgttcaaccg cccgcagggt taccccaacg cagaggcagt tattggtgat
    28021 tttaccgctg tcagcttgct gaatgtttgt tacgacagcc agcactctta tgcccacaac
    28081 gctcagcgta ttcaggtgca actgtgggaa gatctcgaac atcgtcgttt cagtgggatc
    28141 cgcgccagcg aggcgctgat ccatagcggt cgtttccatg cgccgatgcc ggtggtattc
    28201 actagtatgt tggatatcga cggggagacg actgcgcaag accctcggga cacaacccgt
    28261 tttactctgt gtccggacgc caatattacc caaacaccgc aggtgtggct cgatcaccag
    28321 gtgatcgagt tggctgggga gttgcatttc aactgggacg cggtcgagca actgtttgat
    28381 accacgctgc tggatcagat gtttggtgct tattgtcatg cgctgcaggc gctggttgcc
    28441 atgccgcaaa gttggtgggg ggtaaatagt tctctggcgc tgcccaccgt tagtgcaccg
    28501 gtcacgcagg ctcctgcacc tacggccttg ttgcaccatg ggttactgcg tcaggcagca
    28561 ctgacgccac aggaaactgc gctgatcagt cctatccgtg aattgaccta tcgccaactg
    28621 tcgacggcgg cggatcatgt ggcccgcgcc ctgttagcgc tgggcgtgca gcatggcgac
    28681 cgcgtggcgg tggtgatgga aaaaggctgg cagcagattg ccgccgtaca cggcatttta
    28741 cgactgggtg cggtctatct gccagtggat ccggtgctac cgccacagcg tcgccagctt
    28801 ttgctgacgg tgggcgaggt gcgggtacaa gtaacgcagc cgggtctcac gcaattggag
    28861 ccgtcgctgc ccgtgctgat catcgacgac ggaatgctgg acacgcctgc tgcgccgttg
    28921 cctgaagtgg ctggggatgt cacggatctg gcctatatca ttttcacttc cggctccacc
    28981 ggtaccccga aaggagtgat gatcgaccac cgtgcggcca tgaacacgct ggaagacatc
    29041 aacgaacgct ttggcctcaa tgcgcaggat agggtgttcg ggctgtcatc attgagcttt
    29101 gacctgtcgg tttacgatgc ctttgcgcct tttatggtgg gtgcagcgct ggtactgccg
    29161 gaagcaggac gggaaaaaga tccgcgtcat tggcagacag ttatggcaca cggtcatgta
    29221 agcgtctgga atgcagtgcc cgcactgatg cagatgctgt gcgaatacca cagcggcgat
    29281 cggatgagtt atccgacgtt gcgtctggca ctgttgagcg gcgactggat cccgctaacg
    29341 ttaccggagc agatgcgcga gcggctcaat gaaacgatgg acatcatcag tctgggtgga
    29401 gcgaccgagt gcgccatctg gtcggtctac tacccgatag gtgaggtgga atcgacgtgg
    29461 accagtattc cctacggtcg gggtctgcgc aaccagccag tatacgtgct aaatgcgcaa
    29521 ctggaggaat gtccggtcgg ggtggaagga gagatttgca ttggcgggat ggggctggca
    29581 caaggctacc tgaacgatgc agagaaaacg gcggcgagct ttgtctggcg cgaagcgagt
    29641 ggtgagcgaa tttaccgcac tggggatcgc gggcgctact ttgctgacgg gcaagtcgcc
    29701 tttttggggc gcaacgatac ccaagtgaag gtgaatggtt accgtatcga actgggggaa
    29761 atcgagcgct gcattgcgcg acatcccgat gtggagcagt cagtggtggt ggcagtgggt
    29821 aattctcaac atcgtcggct ggtcgctttt gccaaactgc acgatcgcca ccaggcgcag
    29881 gcattgcaag ctaaggaagc ggaggcggcg gcactggcgc agggtattat tgtgaatccg
    29941 gcacagcgtc tagcgttcaa actcaaggag ccacatattc gcgcgctgga tggtctgggc
    30001 attgcactga cggcaccggc ggatagcaca cgttacatca aacgccgcag ctatcgtcat
    30061 ttcagcgcgc aaaaaaccac gctggcacag ttggggcaat tgctgtcggg cttggggcag
    30121 atgcgtctac ccggtctacc ttttgccaag tatgcctatg cgtccgccgg ggggctatac
    30181 ccggtgcaaa cctacgtgta cctgcatcca gacaagatcg aagagggagt atccggtatt
    30241 tactacttcg acccgcgaca gagctgtctt atgccggtag caccagaagt cgagctgaac
    30301 agtggttttc atgccggacc taatcagtct attgccgatc gggcggcatt cacgctgttt
    30361 atggtggctg atatggcggt gatctcgcca ttctatgggc aagaggcagc ttggcacttc
    30421 tcggtgatgg aagcaggtac tctctgccat ttactggaag aagatgcgcc gcgctacgga
    30481 ttggggctgt gccaacttgg gatggcagac ttttccgctg tggcatcgca ttttcaattg
    30541 tcgccacatc atcgctatgt ccattgcacc gtggggggcg cgatagggca agaggcggca
    30601 agtgctgcag cattgctgcg cgatttctcc acctatgaga aaccgaagga aaccgctgcg
    30661 ccgctggaca tgcagagcta caaagatgcc atgctgcgcg gcctgcgtca gcaactgcct
    30721 gactatatgg tgccgagtga tctgatgtta gcgaccgatt ttccgttaac cgctaacggc
    30781 aagctagatc ggcaaaaatt acagctgcag ggcgaacaaa ttgcccacca gcgtgacggc
    30841 gtgggtccaa tccaggtgga cagtgcgtta caacagcggc tggtggcgct ctggcaggag
    30901 gtactcggcg tgagccacgt gtcggccgaa gacgatttct tctcgctggg gggcagttct
    30961 atagaattgg tgcgtattca gcaggcactt gaggcgatta tcgggcagga gattcccatt
    31021 gtcgatctgt tccgtctgcc aaccatcgcg gatgtggcgc gttaccttga tgagcaactg
    31081 cacaatctac ccgccgccca cgatatcgta ttagcgcagg ctgaggtttc gcaggtcagc
    31141 gctgcgcgcg agaatctggc gttgcggcgt aaacgtgccc aacagggaga aaagggcgat
    31201 gagtgagcag agctatcgta gcgcagggac actgttggca cagttggcgt ccggagaaac
    31261 cacctcagtg gcgttggtga atcactattt ttcacgtatg gcgcagttta acaagccgtt
    31321 gaatgcggtg gtgcagcagc actatgcgtt ggcgttggag gccgccgcgc gcgccgatcg
    31381 tgagcgactg gaggggcgcg caaggggcgt gttgcacgga ttaccttgta ctgttaagga
    31441 gtcgtttgac gtgcaagggt ggttaaccac gtccggtgcc cattatctga aagataaccg
    31501 ggcgacgcag gatgctcctt ccatcgcacg cttacgcgct gccggggcga tcctgatggg
    31561 aaaaaccaat gtgccgatga tgactgccga ttggcaaacc tataacgatc tgtatggcac
    31621 cacccataac ctgtgggata ggcaacgttc acccggcgga tcgtccggag gagcggcggt
    31681 ggcggtggcg gcggatttca cccccgtgga atttggcagc gatttgtttg gctcactgcg
    31741 cattcccgca cactacacag gtgtctatgc ccatcgttgt agcttggggt tgatgtcggt
    31801 cagaggacat gtgcccggtg gtggtccgca agcgactgat gagccggatc tctcgacggc
    31861 gggccccatg gcgcgcagcg cggcggatct gcgtctgatg atgcgcgcat tgagcacatt
    31921 ctgggtggaa ccgccgcgta tacccgattt tagccgttat caggccaaag caaactaccg
    31981 cgtgtgcacg tggtttagtg cgccccacca tgagatagat caacagatcg cacagcgttt
    32041 tcaatcgttt atcgacaagt tacgcgcaca gcctggcgtg gaggttgatg atgctatgcc
    32101 cgccgatatc gatcccgacg cactgtttga tatagcagtc aaactcagtg gtcggctggt
    32161 aagtacggcg ctcaatggac gccagcgtct tacagccggg ctggctgcgc tgggcttcag
    32221 gcttgtgggc aagctggcgg atgtgcctga ggggataacg agctattacc agggcatgct
    32281 gaaagacagt ggcgaacagc ggaatacaga caaactccgc cacgaataca gccgggtgat
    32341 agaaaccctt ttcgcgcgtt atgacgtgtt gttgacacct gtcagcccgg tgctggcgtt
    32401 tgcgcacatg cagcagccgg tgcgtaagcg caagcttatc gtcaatggcg aaccgcaaga
    32461 ttacaacgaa catttgttct ggaacatgtt agcaacggtt tttggtttgc ctgccaccgt
    32521 ttacccattg gcgaaaacga tggatgagct tccgtgcggc atacagatca tttccgggca
    32581 ttttcacgat gatgtgacga ttaactttgc tgagttctgc gaaagcatca gtggcggatt
    32641 cacggtaccg gaagggtact agaggcggat atcggtgaca gcgccaataa aggtgcggcg
    32701 catttaatga ataaggagca ggtatgacgg tgaataagct tgagccagac aacggcacgc
    32761 cgaacgatga gctgttcacg gtggcgggta tgtttgacgg ctcgctgtat aaattactcc
    32821 tgcgcatggc gttgccgatg tttgtcggta tgttaacgca ggtgacctat gccatcgccg
    32881 atattttttg gctgagccac attgatgtca ccaacagcgg catcattgcc ggtgtggggc
    32941 tggttttccc cgttggaatg gggttatttg ccatcgccaa cggcattcaa atcggtatgg
    33001 gatctttgct gtcccgcgcc atcggcatgc agcggttgga tcgtgcacag cggattttgt
    33061 cggtcggtat catcatcgcg ctattctttg cgatcgtgat caccgtactt ggctatgttt
    33121 atgcccagcc gctattgcgc tcactggggg ccacgaagag catcatcggc tacgcaacgg
    33181 agttctatta ttattcgctg ctgaccgtgt tcagcatcat gttgattggc gtcatgatgg
    33241 ggctgtttca aggcgcgggt aagatcatgg tcatcatgaa ggcctcattg ttgggggcgt
    33301 tggtaaatat tatgctcgat ccaatcatga tctttgtatt cgattttggt gtgaaagggg
    33361 tggcattggc gtcgtttctg gcgcaactga gtatggtcgc ctatttcatc tacacattga
    33421 tgggattaca tatcgggttg agtatacgca tcgcgttacg cccattctcc tggaaaatct
    33481 accgagagtt tctctccgtc ggcatggcgc agatgctgat gcaactgatc attgcggtcg
    33541 gcatagtgat ttataatttt tttatcgtca ggctggacgt aaacgcgatg gcagcgttta
    33601 cgctcactgg tcgcatcgac tatttcatca tcacgccgat gctggctatc gcgaccgcgt
    33661 tgctgactgt ggtggggcaa aactggggac acggaaatgt cacccgcacg ctgaatgcct
    33721 attgggccgc cgtcgcgctg gccttctcta ttgtgctggt gttagccgtt atgcatattg
    33781 tactggcacc ctggatgtat cccctgttta ctcgtgttgt tgccgtgagt gactatgcgg
    33841 tactgcaaac ccgtatcatg gcactggcac taccctttgt cgctatcagc ctgttagcaa
    33901 gtgaatatta tcaggccatt ggtaagcctt ggtattcggt gttgttgacg ctgatgcgtc
    33961 acgtgtttat atctgtccct gtcgtatacc tgctggcgat tgtgttagag atgcgtatta
    34021 ccggcgtcta ttttggggcg atgagcggca cttttgtggc tgcgttgttg gcgtggcgtc
    34081 tgctgcgtct ttcacctcgt ctgctgcgat ggaatcagga ggcggtgcgc tcccaacact
    34141 tggatatgga ggtggcacca tgatgtcggg caatccgttg tcgtggccac aggaacagtg
    34201 ccacatcatt gatcaactgt atccttacag tgccgtaaac atcattggtg gtgtggtcac
    34261 cattgaggga attgtcgatc tgccccgact acacgcggcc atccagtccg ccattcggca
    34321 atttgatgcg ttgcgtatgt ggtttgtgat gggagaggag agtgaagtgg tcagccaagt
    34381 gcagccttat cactggcgcg atatccgcca tttgactttt tccccagatt atgacaagga
    34441 gaacctacgc ccagcggcga tcgaaacatt tgttgatgag tggttccggc agccctttac
    34501 cctgctggcg cacgatttgt tcgagtttgt cacctttacg tgcggtgaac aatacagcgg
    34561 ttatttattt aaagcccatc acggcattgc tgatggttgg tcaatggcgt tgttgtcaaa
    34621 ccatgtcaag cgagcatacg aacagcagga cgtaccagat gatgcgtcac cagcctatag
    34681 cgcatttctg gcgcagcagc aatcttatca ggcgtcaacg cgctttgccg ttgatcgtgg
    34741 ctggtggcgt gactatatcg acgaataccg cgactgcttt ccagacagtt cccccatcgt
    34801 tactactgag ggcatcagtt gttccacttg gcttgagcca gccatgatta accgtctcta
    34861 caggttgtgt aatcgttatg gctgtacgct taatacgctg tttattgcac tcttcgctct
    34921 ctatcgcgcc agagtatggg gggaggaaaa aggcgtaata ggcgtgccgt tagccaatcg
    34981 gcatactcgc gaggcgcggc gttgctttgg catgtttact aatcaattgc cgctggccta
    35041 tcgactggtg cgcacggaac gattctgtga acgggtggcc tttttccagc gcgaactgaa
    35101 acgcggtttt aaacacagca aatatcccat taccttattc aaccaggatt tggcggaaca
    35161 ggggggaggg aagttgcggg cgttcgatta ttgcgttaat tattataact ttacctatga
    35221 acgccacatt gcaggtgccg cgcaacgcgt ggagagttac tacagcggcg agcagtccta
    35281 taagctacag attgttttgc aaactgttaa taatcataaa gaaagcctta ggttgagtct
    35341 ggaggcgctg cgtagcgctt tcactccgca ccaactgaca gcgatgaaaa atgggctgtt
    35401 ggatttagtc actgcattgg atcggcaacc tgacgctcgt ctaggcgatc tcgaagtcta
    35461 tcctgcgccg cacgtcgcat tggcatgtgg ctcactcaaa ccctcattta cttcccgatt
    35521 tgcagcgcaa gttgtggagc atggtgatcg cacagcacta attgataatg aacagtcttt
    35581 gacttatcgg cagttggatg acgctgttga gcgggttgcg cgttatctgc gccaacaggg
    35641 gatcggacgc ggtcaagtgg ttgggattat cgctgagcat agcgctcaaa cggtgatggt
    35701 tatttatggt attttgcgct gcggcgcggc atttctgccg ctaaatcccg cgttgccaac
    35761 gacccgcctt tacgccatgt gtcgtaaagc gcaggtagcg catatccttt acgatccggc
    35821 gatgcatgaa ttgacgcagg cgctggcgtt tcccgcgtcc agcttgctcc aggccttagc
    35881 cacttcggcg cttgctagag aaccttggcc agctattgaa ccgcaggatc tggcatatgt
    35941 gctgtttacc tccggatcga ccggcgagcc taagggcgtg caagtctctc atggcaatct
    36001 ggctaactat ctccatttcg ccgccgaacg ttactttaca gcgcaggacc gggctgcgct
    36061 ctactcttcg ctgtccttcg atttgaccat cactacgctg ttcgccccct tatgtgtggg
    36121 cgctagcatc agcgtttgtc ggcacgccga gagcgaaaca ttgctgcgca tggccgtggt
    36181 tgatcagccc aacacagtga tcaaactgac gcctgcacac ctgcggttgt tgtgtgcggc
    36241 tggcataagc agtgagcaga ttcgtacctt ggtggttggc ggggaggatt tcaagcggga
    36301 tctggcgcgt aaggcggctg ctctctttcc tcaggccgtg atctataacg aatatgggcc
    36361 gacagaggcc accgttggtt gcatgatcta tcgctacacc gggcaagaaa cgctgccttc
    36421 attgcccatc ggtatggcga tcgacggttg ccaggtagca atttgctccc cttggggctg
    36481 tccggtaccg gaaggcgaaa ctggtgaatt ggtgatttat ggcgcgtcag tgacgcaggg
    36541 ctatatcgat gcgccgcaac aaacggccgc cgcctacctt aaagatacca atggggtgat
    36601 gattggctat cgcagcggtg atattggcta cgccatcgct cctaatacgc tggtttatca
    36661 gggacgtaaa gacgatcagg tcaaaattaa tggctatcgt attgagctgt gcgaaatcga
    36721 acaagcgttg ttgagcgcac ctcaggtaga aagtgcggcg gtggcggtga ttgatgatgt
    36781 gcaggggcag cacagcgggt tgctagcctg cgtgacaccg tcatctgttg atgtagctac
    36841 cgtaatgcaa catctgcgtc agcaattacc cacctacatg caacccaagc agtgctgtgc
    36901 tatcgcccaa ctgccgctgt cgcacaatgg caaggtggac gtgcgtcaga tggtcgcaac
    36961 ggtccgaaac accgcgcctg catcgggtag cgagcggctg ggagatgcgg cgataaggca
    37021 ttcagttcgg gtatgtgtgg aaggggcgct ggagcaaact gagtttgatg ataacgaaaa
    37081 tctttatgtc ttggggttgg actccatcaa gagtatccag attgcagcgc agctcaggca
    37141 ccacggctgg acgatgtcag cggtgcaggt gatggagtgt ggaacagtga acgccatctg
    37201 tgagtttctc gctagtcata caacggtgtc acagctcgcg cagtatgctc ataacacccg
    37261 tatcgatcta cccgcattgc gttggtttac gcagttggcg ttgccagtgc ccaatgttta
    37321 taaccatgtc attgtgctga aagtgttgcc gggctgcccg ctcgagcagt tacacaacag
    37381 gctgcataca ttaatccagc agcagccagc tttgcacagt gcgctggacg ctgaggggcg
    37441 gttgctagta tgcgacccta atgtgtgtta tcccaacgag gtgctcacgg aatattcgac
    37501 ggcgcagtgg acgttggcag aggtgatcgc tcagtgcaat agcatgcttg acgtgacaaa
    37561 tggtcgagtc tttacggcag ctctgttaca cgccccgcag cctgcgtcta gcacgctggt
    37621 actctgtgcc catcacttgt gcgtcgatat gcactcttgg tatttaatcc tcagcacgct
    37681 ggacgccgtc agcactgtca atggcaccag caacagcgga ctgcatcgct ggaatgacta
    37741 tctggcgagc aaaacggtag attccgccac acacgaaagc tggcgcacag tatgtcaaac
    37801 gctaccgctg catttcccac ccgtgtcatt gccagatgac tcattacctc gtacgcgcgc
    37861 ttggcgtgaa gactttcgcc atccttgcgt caggaggttg tttgaatcca gtggtaatac
    37921 cgcgtacagc gccgaaacct atgtgctaac ggcattggcg ctggtgctgc gttactacag
    37981 cgaagagcct tggtgtcgca tcgagatgga aggcatggga cgcggctgct ggcccgatga
    38041 gcctgacgtt gcggatacgg tcgggtggtt tactctattt tatccttggg cgatccctct
    38101 gcatggcgat atggcgacac tgttatctgc gatcgcttcc gatttggcaa aacgcacgca
    38161 tggtggggga gattacggtc tgctgcaaat gcggcatgca ccggaagact ctcttgcgca
    38221 agggatccga atgaattaca tcggtgtgca ggcgcaacct tcactccgct attttcatat
    38281 tgatcacttt aatagtgata tctataccgc gcctgaaaac gcgctcggct gcgtgctgga
    38341 gtttaatatt gcgcgctcgg cagctgatgg tctctccttc cactgccgtt tcgatcccac
    38401 gcgcattgcg ctgaatgatg tgcaactcct gctggcgcgc tacaagaaca gcctcactga
    38461 tctggatgcc tggctgtgcc aacacagcgc cacgctgacg ggcgcgccga ctttgtggac
    38521 actataaaca cgatgccaac ccttaaggaa tacaacaatg gcaaaggatg attttacctg
    38581 tggctcactg gatattgcca ttattggcat gagcgggcgt ttttccggtg cggaatcggt
    38641 gcccgagtgg tgggataagt tgctggcggg tgaggagttc actcaaccga catgcacaga
    38701 agacgataat gggaaccctt ggattaggct gcgcaatata atcaccgctc cctatgactt
    38761 cgatgctgca tttttcaata ttccgcctgg cgaagccctg ttgatggatc cgcaacaacg
    38821 aatatttctt gaatgctgct acaacgctct tgagcatgcg ggctatattc ccacgcaact
    38881 taagcgagtc ggcgtctatg gggcgaccta cgccaataac tattttatcg atcgcgtcta
    38941 tccatacctg aagatgagcg gcgatcacca ttatcttcag gcgcagattg gtaacgaaaa
    39001 ggattacttg tgtgctcagg ttgcctacaa gttggggttt accggacccg ctgtgagtgt
    39061 acagaccgcc tgttccagtt cgttggtggc agcatattta gcatgcgagg ggttattgac
    39121 ttttcaagcc gatgttgcgt tggcaggtgg ggtcaccttg ggttttttac aggcgcacgg
    39181 ctacagtccg caaggtgata agctggtatc gcaagacgga cactgcgccc cgtttagcgc
    39241 tgaggcgacc ggcacggtat acagcagcgg tgcgggagtt gtggtgctaa aacgtcttga
    39301 ggatgcacta cgcgaccaag acagagtgta tgcggtgatt aaaggaggtg cggtaaataa
    39361 cgacggcggg cgtcggctgg ggtttgtcgc ccccagcgta gaaggacagg tagaagcgat
    39421 caatacggcg cttgccgccg cagaagtcgt accgactgat atcgcactga tcgaaacgca
    39481 cggtaccggt accccgttgg gtgatgagat tgagcttgaa gctctgcacc gggttttcgc
    39541 accggcatgt gcgccgcaca gcattcaatt gggtgcggta aaagccaact tgggtcacct
    39601 gggagtcgct tccggtatcg tcagcttaat gaaaaccgca ctgacgctat atactggttt
    39661 agttcccccg cagatcaacc tagtaaataa acataaaaaa ctgttgcagc cagcctcgcc
    39721 attttatctc agtgatgtag tgacttctgt cccacaaacg aaaaggatcc atgccaccgt
    39781 tagttcgttt gggctgggag ggaccaatgc tcatttggtt ctgcaaaact ggtgcgagac
    39841 gcctgcgcaa gcggtgcaag aaaatgagcg ccgcctgttt ttcttcagcg ccaaaacgcc
    39901 attagccctt agacaacagt tggatgctca ttatcacgct ctggcgacct atgcggaagc
    39961 agataaagat cgtatcgcct atactctcgc gcaacgccgg gcacatttcc cgtatcgctg
    40021 tgcgctggcg gcagacagtg tggtagcgtt gcgtgcaagt ttggcgaaac tgcgggatgc
    40081 tgacatgtct tttaccccta tcaatatgga aactaccttg gtgttcctat atccggaccg
    40141 ggacgataag ctggagagtg ccctgacgca tttgctggct tgtcaaccta acttgcgcca
    40201 gcggcaccag cgcctgtctc aggatgttgc acaaatctgc gaaccagccg attggacgcc
    40261 agcattgcgt cagtttatcc agcaggtgag tcttagtgaa tggctgatcg aacaaagtat
    40321 ctcgcctgtg cagcacatcg gttacctgac gggggctgca gcagcgcagt atgttgcgcg
    40381 catcatctca ctggaaaacg ctgttcagca ggtgattgtg gctgaaacga caccggagca
    40441 gacgctggcc ggcaacagtg aactgagcga aatattggct aatctggcgg tgacggaggg
    40501 aacgctgatg ctggaaatcg gtcgcgcggg gacgttttcc atattgtatc accagcatgc
    40561 gcagtgggtt gggcaaacag tattttcccc catgctgaat acggatacgc ctgaggacat
    40621 cctgccgctg ttgggcactc tttggcaacg aggagtgact atttgtttac cggaaatgcc
    40681 agccgtgcag actataggct tacccggtta ttcgtttgat cgcgtccggt atgaaattca
    40741 gtccagtgat gcgagagaga acgctatgtt accagtgagt tatttgtcgg tcagcgactt
    40801 tgtggagaaa acctggcgtt cattgttatg catcgatcat tatgacgaac acgcggttat
    40861 tttcgaatac ggagcgacct cgatgcacgt tatctctttc gtcgacagct gtaaccacat
    40921 ttataaaatc ggactgactg ccgccgatat ttatgccaga cccgctatcc gtgagcacag
    40981 tgaatttatc tccgaatgtg ttgatggtat tttatgaggt gttcacagga tgacaataat
    41041 ggaacacgtt agcattaaaa cattatatca tctcctgtgc tgtatgctgc tctttatttc
    41101 cgctatgtgc gctttggcgc aagaacatga gcctatcggg gcgcaagatg agcgcctgtc
    41161 gacattaatt caccaacgga tgcaggaggc caaggtccca gccctttccg taagtgtgac
    41221 cattaagggg gtacgtcagc gatttgtcta cggtgttgcc gatgtggcta gtcagaaagc
    41281 gaatactcta gacacagttt acgagctggg atcgatgagt aaggcgttta ccggacttgt
    41341 ggtgcaaata ctgattcagg aaggcagact ccggcaaggg gatgatatca ttacctatct
    41401 gccggaaatg cgcttgaatt atcagggaaa acctgcttcc ctgaccgtgg ctgatttcct
    41461 ttatcataca tcaggattgc ctttttcaac actggctcgg ctggaaaacc ctatgcctgg
    41521 gagcgctgtg gcacagcaac tgcgcaacga gaatctgctg tttgcgccgg gtgcgaagtt
    41581 tagctatgcc tccgccaatt atgatgtgtt gggcgcggtg attgaaaatg tgacgggaaa
    41641 aacctttaca gaggtcattg cggaacgact cacgcagccg ctgggcatgt cggcgactgt
    41701 ggcagttaag ggggatgaga ttattgtcaa caaggcaagc ggctataaac tgggattcgg
    41761 caaacccgtt ctgtttcatg cgcctctggc ccggaaccat gttcctgccg cctatatcca
    41821 tagcactctg cctgatatgg aaatatggat agacgcctgg ttgcacagaa aggctttgcc
    41881 ggcaacgctg cgtgaggcga tgagtaacag ttggcgtggt aatagtgatg ttccgcttgc
    41941 cgcagacaat cgtatcctct atgccagcgg ttggtttatc gaccagaatc aaggccctta
    42001 catcagtcac ggtgggcaga atccaaactt ttcttcttgc attgcgttgc gaccggatca
    42061 gcagattggc attgttgcgc tggcaaatat gaattcgaat ctgatactac agctttgcgc
    42121 ggatatcgat aattatctgc gcattggcaa atatgctgac ggcgctggtg atgcaattac
    42181 agccaccgat acccttttcg tctacctcac gttgttgctg tgtttttggg gggcggtggt
    42241 tgtagtgcgc ggtgctttcc gtgtttatcg cgcaacggcg catggccctg gaaaacagca
    42301 gaggttacgt ttacgcgtac gtgactatat catcgccttg gcggttcctg ggctcgtggc
    42361 cgccatgctc tatgtcgcac cgggtatact atctccagga cttgactggc gttttatctt
    42421 ggtatggggt ccatcgagcg tgttggcgat accgttcgga attatcctgt tagctttcgt
    42481 tctgacatta aatcatcaaa ttaaacgaat tctattacac aacaaggagt gggacgatga
    42541 gtaatatcag tttgtattgt ttgccatatt caggtggttc tgccgccatg tattataaat
    42601 ggcgtagcgt gctgtcggac aatattactt tgcggccttt agaacctgcg gggaggggaa
    42661 ctagaatacg ccagccgctg tgtcttacga tggtggatgc cgtcgctgac ctttatcaac
    42721 aatttgtgaa acactacaca ggtggagact acgccatttt tgggcatagt ctcggaggga
    42781 tcatggcctt cgaactggtg cattatattc tcgatcatgg acatgacatg ccatgcgcgc
    42841 tgtttttttc cggctgtcgc ccacccgatc gggcctctca tgaagtaata ctgcatacct
    42901 tgcccgatca ggcgtttatg gaagagatcg tcaagctggg cggaactccg gttgatgtct
    42961 ttcgtaataa agagttaatg acaattttca cccccatcat taaaaacgat tatcggctct
    43021 atgagcagta tgtatttcag gccaaggcgc gcacattaac ctgtccgatc gtgctatttc
    43081 atggcgatgc tgacaatctg gtaatgcagg atgaattact tgcatgggaa aaattcacca
    43141 cacgaaagac gcggactatt atatttcctg ctgccgatca tttttttgtc gataagcatt
    43201 ttgaacaggt ggtaggttat gtgaaccaga cgattgaatc actcgaaata gtagggtaga
    43261 aatagttgtg taactataca agga
//
LOCUS       DADTFG010000018        43270 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.018, whole genome shotgun sequence.
ACCESSION   DADTFG010000018 DADTFG010000000
VERSION     DADTFG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.018"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            83..649
                     /locus_tag="J1138_000975"
     CDS             83..649
                     /locus_tag="J1138_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA8452811.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            798..1919
                     /locus_tag="J1138_000976"
     CDS             798..1919
                     /locus_tag="J1138_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBA8452812.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSFPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYTRLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            1919..5007
                     /locus_tag="J1138_000977"
                     /pseudo
     CDS             1919..5007
                     /locus_tag="J1138_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            5030..6403
                     /locus_tag="J1138_000978"
     CDS             5030..6403
                     /locus_tag="J1138_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBA8452813.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            6412..7575
                     /locus_tag="J1138_000979"
     CDS             6412..7575
                     /locus_tag="J1138_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HBA8452814.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWVQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(7623..8429)
                     /gene="sapF"
                     /locus_tag="J1138_000980"
     CDS             complement(7623..8429)
                     /gene="sapF"
                     /locus_tag="J1138_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBA8452815.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(8431..9423)
                     /gene="sapD"
                     /locus_tag="J1138_000981"
     CDS             complement(8431..9423)
                     /gene="sapD"
                     /locus_tag="J1138_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBA8452816.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(9423..10313)
                     /gene="sapC"
                     /locus_tag="J1138_000982"
     CDS             complement(9423..10313)
                     /gene="sapC"
                     /locus_tag="J1138_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBA8452817.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(10300..11265)
                     /gene="sapB"
                     /locus_tag="J1138_000983"
     CDS             complement(10300..11265)
                     /gene="sapB"
                     /locus_tag="J1138_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBA8452818.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(11262..12905)
                     /gene="sapA"
                     /locus_tag="J1138_000984"
     CDS             complement(11262..12905)
                     /gene="sapA"
                     /locus_tag="J1138_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBA8452819.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(13218..13463)
                     /locus_tag="J1138_000985"
     CDS             complement(13218..13463)
                     /locus_tag="J1138_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBA8452820.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(13597..13704)
                     /locus_tag="J1138_000986"
                     /pseudo
     CDS             complement(13597..13704)
                     /locus_tag="J1138_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine importer"
     gene            complement(13890..14867)
                     /gene="pspF"
                     /locus_tag="J1138_000987"
     CDS             complement(13890..14867)
                     /gene="pspF"
                     /locus_tag="J1138_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBA8452821.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            15033..15701
                     /gene="pspA"
                     /locus_tag="J1138_000988"
     CDS             15033..15701
                     /gene="pspA"
                     /locus_tag="J1138_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBA8452822.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            15755..15979
                     /gene="pspB"
                     /locus_tag="J1138_000989"
     CDS             15755..15979
                     /gene="pspB"
                     /locus_tag="J1138_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBA8452823.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            15979..16338
                     /gene="pspC"
                     /locus_tag="J1138_000990"
     CDS             15979..16338
                     /gene="pspC"
                     /locus_tag="J1138_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBA8452824.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSRELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            16347..16568
                     /gene="pspD"
                     /locus_tag="J1138_000991"
     CDS             16347..16568
                     /gene="pspD"
                     /locus_tag="J1138_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBA8452825.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            16643..16957
                     /gene="pspE"
                     /locus_tag="J1138_000992"
     CDS             16643..16957
                     /gene="pspE"
                     /locus_tag="J1138_000992"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBA8452826.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            17167..18846
                     /locus_tag="J1138_000993"
     CDS             17167..18846
                     /locus_tag="J1138_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBA8452827.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKFVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSMRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTNCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLSYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            18860..20152
                     /locus_tag="J1138_000994"
     CDS             18860..20152
                     /locus_tag="J1138_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8452828.1"
                     /translation="MIKSKIVLLSALVSCTLISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     RLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            20173..21054
                     /locus_tag="J1138_000995"
     CDS             20173..21054
                     /locus_tag="J1138_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA8452829.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIEATFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            21041..21883
                     /locus_tag="J1138_000996"
     CDS             21041..21883
                     /locus_tag="J1138_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA8452830.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSEHFIKSGLTAG
                     GVKG"
     gene            21914..22966
                     /locus_tag="J1138_000997"
     CDS             21914..22966
                     /locus_tag="J1138_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBA8452831.1"
                     /translation="MKKLVATAPRVAALVEYEDRAISANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTAYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPITRRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            22985..23773
                     /locus_tag="J1138_000998"
     CDS             22985..23773
                     /locus_tag="J1138_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA8452832.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAENPAQAYRDSLAWLRTC"
     gene            23783..24838
                     /locus_tag="J1138_000999"
     CDS             23783..24838
                     /locus_tag="J1138_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA8452833.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            24835..27102
                     /gene="ycjT"
                     /locus_tag="J1138_001000"
     CDS             24835..27102
                     /gene="ycjT"
                     /locus_tag="J1138_001000"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBA8452834.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLTGLYHRAGKGEINKLVNLPDVVGMEIAINGEIISLSREAWQRELNFASGELR
                     RNVVWRTSNGTGYTIASRRFVSADQLPLIALEISITPLDANASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELLLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            27099..27758
                     /gene="pgmB"
                     /locus_tag="J1138_001001"
     CDS             27099..27758
                     /gene="pgmB"
                     /locus_tag="J1138_001001"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBA8452835.1"
                     /translation="MKLQGVIFDLDGVITDSAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            27772..28854
                     /locus_tag="J1138_001002"
     CDS             27772..28854
                     /locus_tag="J1138_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8452836.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAREVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIGIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            28899..29804
                     /gene="ompG"
                     /locus_tag="J1138_001003"
     CDS             28899..29804
                     /gene="ompG"
                     /locus_tag="J1138_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBA8452837.1"
                     /translation="MKKLLLCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERAFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(29855..30853)
                     /locus_tag="J1138_001004"
     CDS             complement(29855..30853)
                     /locus_tag="J1138_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA8452838.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            31009..32406
                     /locus_tag="J1138_001005"
     CDS             31009..32406
                     /locus_tag="J1138_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBA8452839.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            32403..33464
                     /locus_tag="J1138_001006"
     CDS             32403..33464
                     /locus_tag="J1138_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBA8452840.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            33612..35153
                     /gene="tyrR"
                     /locus_tag="J1138_001007"
     CDS             33612..35153
                     /gene="tyrR"
                     /locus_tag="J1138_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBA8452841.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(35197..35703)
                     /gene="tpx"
                     /locus_tag="J1138_001008"
     CDS             complement(35197..35703)
                     /gene="tpx"
                     /locus_tag="J1138_001008"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBA8452842.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            35822..36787
                     /gene="ycjG"
                     /locus_tag="J1138_001009"
     CDS             35822..36787
                     /gene="ycjG"
                     /locus_tag="J1138_001009"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBA8452843.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(36762..37490)
                     /gene="mpaA"
                     /locus_tag="J1138_001010"
     CDS             complement(36762..37490)
                     /gene="mpaA"
                     /locus_tag="J1138_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBA8452844.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(37781..38419)
                     /locus_tag="J1138_001011"
     CDS             complement(37781..38419)
                     /locus_tag="J1138_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA8452845.1"
                     /translation="MKNILILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(38416..38490)
                     /locus_tag="J1138_001012"
     CDS             complement(38416..38490)
                     /locus_tag="J1138_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452846.1"
                     /translation="MKHIQIRNSDMAWHIAANNLGKVS"
     gene            complement(38490..39410)
                     /locus_tag="J1138_001013"
     CDS             complement(38490..39410)
                     /locus_tag="J1138_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA8452847.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSDARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSKDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            39548..40447
                     /locus_tag="J1138_001014"
     CDS             39548..40447
                     /locus_tag="J1138_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8452848.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMSAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            40784..42397
                     /locus_tag="J1138_001015"
     CDS             40784..42397
                     /locus_tag="J1138_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8452849.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRFLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(42448..43194)
                     /locus_tag="J1138_001016"
     CDS             complement(42448..43194)
                     /locus_tag="J1138_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBA8452850.1"
                     /translation="MLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFG
                     MSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVA
                     EIGWRITKIKTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIV
                     EAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKI
                     IDIVQSHGADFAFPSQTLYMDNITPPDQGR"
BASE COUNT        10698 a        10435 c        11490 g        10647 t
ORIGIN      
        1 actttccatt actttggaga aacgtataat tagcaaaaat gagaacgatc attctcacca
       61 agtgataggc caggagacag aaatgaccag caagctggag atacgccaca aacagcgtca
      121 ggatgaaatc attaacgccg cccgtcggtg ttttcgtcgc tgcggatttc atgccgccag
      181 tatgtcgcaa attgccagcg aagctcagct tagcgtgggt cagatatatc gttacttcgc
      241 caacaaagac gccattattg aagagatggt tcgtcgtatc atcgactttc gtattgccca
      301 aatggacatt gacgcgcgta ccgatcactt cccggaagtc cttgccttgc gtaaatcctt
      361 aaatgaggac gacgacgcgc tgatgctgga agtggctgca gaagccacac gcaacccgcg
      421 ggttatggca atgcttgaag aagctgacgc cagaatgttc gctaacggtt gtgcacatat
      481 gaaaagaatg catccccatc ttagcgacga gcacattcgt tgctgcgttg aagtgtttgc
      541 taccatgatg gaaggtacgg tttatcgtcg tttaacaccg caaaaaagcg atccacaaca
      601 tctgcaggaa atatatcagg atattgttag tatgttgata aataaataag aacagaatta
      661 accaatagcg ggggatatta atccccctta tttcattgcg aaattcattt cgcgatgcgg
      721 gttcgttata actgaatatc cctgacatat cctcattaat ttcaatgagg agggtatttc
      781 tcaccctgga gaaaataatg aagtatatag caacatctgt agtggcaatg ctgctcttat
      841 cgggttgtga taatacgcaa agtaacaatt cattcccgtc agaaacagaa gtcggcgttg
      901 ttacgctcaa atctcaaccg gtttcggtag tcagtgaatt aaccggacgt accagtgctg
      961 cgctcagtgc tgaagtacgt ccgcaggttg ggggaattat ccagaaacgc ttatttaagg
     1021 aaggtgatct ggtcaaggct ggacagcctc tctaccagat tgatgcggcc agttatcagg
     1081 ctgcatggaa tgaagcccgg gcagcattac aacaagcaca ggcactggta aaagccgatt
     1141 gccagaaagc gcagcgttat acccgactgg tgaaagagaa cggtgtttca caacaggatg
     1201 ctgatgatgc tcagtctacc tgtgcacaag ataaagccag tgtagaggcg aaaaaagccg
     1261 cactggaaac tgcgcgcatt aatcttgact ggaccacggt aaccgcaccg atttcagggc
     1321 gtattggcat ttcgtcggta acccctggcg cactggtgac cgcgtcgcag gatacagcgt
     1381 taacgactat tcgtggtctg gatacaatgt atgtcgacct cactcgctcc agtgtcgatt
     1441 tattacgtct gcgtaaacag tcactggcga ccaacagtga caccatgagc gtctcactta
     1501 ttctggaaga tggcacaacc tacagcgaaa aagggcgtct ggaactcacc gaagtcgcgg
     1561 tggatgagtc taccggttcg gtgacattac gggcaatttt ccccaatcca caacagcagt
     1621 tattaccggg aatgtttgtt cgcgctcgtg tcgatgaagg cgtgatggaa gacgctattc
     1681 tcgcgcctca acagggcgtt acgcgcgatg ctaaaggcaa tgcaactgcg ctggtggtga
     1741 ataaagacaa taaagtagag cagcgaacgc tcgaaacggg agaaacgtat ggtgataaat
     1801 ggctggtgct gaacggcctg cacaacggcg accgactgat tgttgaaggt tctgccaaag
     1861 tcacttcagg gcagaccgtc aaggctgttg aagttcaggc taatggaggc aacgcctgat
     1921 gttttcgcgc ttttttgttc gccgcccggt ctttgcctgg gttatcgcca ttttgattat
     1981 gttggcgggg attctggcta ttcgcacatt gcccgtcgcg caatatcctg acgttgcacc
     2041 gccgaccatt aaaatttcag ccacttatac tggtgcttct gccgaaacgc tggagaacag
     2101 cgtgactcag gttatcgagc aacaactcac tgggcttgat aatttactct atttcagctc
     2161 aaccagtagc tctgatggtt cggtcagtat taatgtgacc tttgaacaag gtaccgatcc
     2221 agatactgca caggttcagg tgcagaataa aattcagcag gcggagtcgc gcctacccag
     2281 cgaagtgcag caaacgggtg ttacggtgga gaaatcacaa agcaactttt tgctgattgc
     2341 tgccgtgtat gacaccactg acaaagcctc cagttcggat atcgccgact ggctggtcag
     2401 taacgttcag gaccccctgg cgcgtgttga aggtgtgggg agtctgcaag tctttggcgc
     2461 ggaatacgct atgcgcatct ggctcgaccc ggccaaactg gcgtcttact cgctgatgcc
     2521 ttcagacgtg caaagtgcta ttgaagcgca aaacgtgcag gttactgccg ggaaaatagg
     2581 ggcattgcct tcaccggata ctcagcaact gaccgcaacg gtacgtgcgc agtctcgttt
     2641 gcagacggtg gatcagttca aaaatattat cgtgaaaagc cagtcagacg gcgcagttgt
     2701 tcgtataaaa gatgtggctc gcgttgagat gggcagtgaa gattataccg ctatcggcaa
     2761 acttaacggt cacccgtctg ccggggttgc tgtaatgctt tcgcccggtg cgaatgcgct
     2821 gaatacggcg acgctggtca aggataagat tgccgaattc cagcggaaca tgccgcaggg
     2881 atacgacatc gcatacccga aagacagcac tgaatttatt aaaatctccg tagaggacgt
     2941 aattcagacg ctgtttgaag ccatcgtatt ggtggtttgc gtgatgtatt tattcctgca
     3001 aaacctgcgt gccacactga ttccggcgtt agctgtcccc gtcgttttgc tgggcacatt
     3061 cggcgttctt gcgttgtttg gctattcgat taataccctg acgttgtttg caatggtgct
     3121 ggcgatcggc ttgctggtgg acgatgccat tgtggtggta gagaacgtcg aacgcattat
     3181 gcgtgacgaa gggttgcccg cgcgtgaagc cacggaaaaa tcaatgggcg agatttctgg
     3241 cgcactggtt gccattgcgc tggtgttgtc agccgtattc ctgccgatgg ccttctttgg
     3301 gggatctacg ggggtaattt atcgtcagtt ctccatcacc atcatctccg caatgctgct
     3361 ttccgtggtg gtggcattga ccttgactcc cgccctgtgc ggttccgtcc tccagcatgt
     3421 tccaccacat aaaaaaggct ttttcggcgc atttaaccgc ttttaccgcc gtactgaaga
     3481 taaatatcag cgaggcgtaa tttatgtcct gcgccgtgca gcccgaacga tggggcttta
     3541 tgtcgtgctg ggtggtggaa tggccctgat gatgtggaaa ctgccgggca gtttcttacc
     3601 cactgaagac cagggcgaaa tcatggtgca gtacacgctg ccggccggtg caaccgctgc
     3661 ccgtacagca gaagtgaatc gccagattgt tgactggttc ctgattaacg agaaagcaaa
     3721 taccgatgtc atctttaccg ttgatggttt cagttttagc ggcagcggac agaacaccgg
     3781 gatggcgttt gtttcgttga aaaactggtc tcaacgtaaa ggggcagaaa acaccgccca
     3841 ggctatcgcc ctacgggcaa ccaaagagct gggcacaatt cgtgatgcca cggtatttgc
     3901 gatgacgccg ccagccgttg atgggctggg gcaaagcaat ggttttacgt ttgaattgtt
     3961 agctaacggt ggaaccgatc gtgaaacact gctgcaaatg cgtaatcagt tgatagaaaa
     4021 agcgaatcaa agtccggagt tacattctgt acgcgccaat gatttgccac aaatgccgca
     4081 attgcaggta gatattgata gcaataaagc ggtgtcatta gggttgagtt tgaatgatgt
     4141 caccgacacc ctgtccagtg cgtggggcgg tacttatgtg aatgacttta ttgaccgtgg
     4201 tcgtgtgaaa aaagtctaca ttcagggcga cagcgaattt cgttccgcgc cgtcagactt
     4261 aggtaaatgg tttgtgcgcg gcagcgataa cgccatgaca ccattctctg cttttgcgac
     4321 cacccgctgg ctgtatggac cggaaagact ggtgcgctac aacggctcgg cagcctatga
     4381 aattcagggc gaaaacgcga ctggctttag ttccggcgat gcaatgacaa aaatggagga
     4441 actggcaaac agtcttcctg cgggaacaac atgggcctgg agtggtttgt cattgcagga
     4501 gaaactggcc agcggtcagg cattaagtct gtatgcagtt tctattctgg tggttttcct
     4561 ctgccttgca gcactgtacg aaagctggtc agtcccgttc tcggtcattc tggtgatccc
     4621 tctggggctt cttggcgcgg cgctggcggc ctggatgcgt gatttaaaca acgacgttta
     4681 cttccaggta gcgctattaa ctactatcgg cttgtcatcg aaaaacgcca ttctgattgt
     4741 ggaatttgct gaagcggcgg ttgcagaggg ctattccttg agtcgtgcgg cattacgcgc
     4801 ggcgcagact cgtttacgcc caataatcat gacctcgctg gcgtttattg cgggggtaat
     4861 gccgctggcg atagcaaccg gcgcaggggc gaacagccgc atcgccattg gtacgggcat
     4921 tattggcggc acgctgaccg ctacattact ggccattttc ttcgttcctc tgttttttgt
     4981 actggtgaaa cgtttgtttg ccggtaaacc gcgccgtcag gagtaagtta tgttgcgtcg
     5041 ttctcttatt tttctggtgt tgttgagtgc gggatgtgtc tcgctcgatc ctcactacag
     5101 tacgcctgaa tccccaatcc cggcgacctt gcctggagca cagggccagg ggaaggcaat
     5161 cagccatgac tggcagcagg tgatccacga tccccgattg cagcaggtag taaccattgc
     5221 actgaacagt aaccgcgatg tgcaaaaagc gattgctgat attgactcag cacgggcgct
     5281 ctatgggcaa actaatgcgt cgttatttcc gacggtgaat gcggccttaa gttcgactcg
     5341 cagtcgttcg ctggcgaatg gtacgggaac aactgccgag gctgacggca cggtgtccag
     5401 ttatacgcta gatctgtttg gtcgtaatca gagtttatct cgggcggcgc gggaaacctg
     5461 gcttgccagt gaatttacag cccagaacac tcgtttaacg ctgattgctg aaatcagcac
     5521 cgcctggctg acactggcgg ccgataacag caacctggcc ctggcaaaag aaacgatggc
     5581 cagtgcagaa aactcattga aaattatcca gcgccagcaa caggttggca cggcggcagc
     5641 gacagatgtc agtgaggcga tgagtgttta tcagcaagcg cgcgccagcg ttgccagcta
     5701 tcagacacaa gtgatgcagg ataaaaatgc gctgaactta ctggcaggta caacgcttgc
     5761 ggaaaatctg ctaccgggaa cactggaaag cctgccggaa caaatgataa gtctggttcc
     5821 tgcgggagta tcatctgatg ttctgctgcg tcgcccggat atccaggaag ctgaacataa
     5881 tttgaagagc gccaatgcag atattggcgc ggcgcgggct aactttttcc cgacgatttc
     5941 gctgactgcc agtgccggtg ttggcagtga cgcattgtcg tcgttgttca gccacggaat
     6001 gcagatctgg tcatttgcgc catcggtcac attaccgctg ttcactggcg gaagtaatct
     6061 ggcgcagctt cgttacgcag aagcacaaaa gcgtgggctc attgccacct atgaaaaaac
     6121 cgttcagagc gcatttaaag atgtcgctaa cgcactggcg cggcggacca cgcttgaaga
     6181 gcaactggat gcacagcgcc agtatgttaa agctgagcaa caaacggtcg atgttggctt
     6241 gcgtcgctat caggcgggtg ttggtgatta cctgaccgtg ttgacggcgc agcgcagctt
     6301 gtggagcgca cagcaggagc tgctggcact gcaactgact gattttacaa accgaatcac
     6361 gctctggcag tcgctgggcg gcggtatgtc atcactcaag taaggaataa tatggctaga
     6421 gtctctcttt catgggcatt gattcttggt cttttagccg gtatcggccc gatgtgtacc
     6481 gatctttatt tgccggcttt gccggagatg tctgagcaac tggcggcaac cacgaccata
     6541 acgcaattaa ccctgaccgc atcactgatt ggtcttggcg tcggacaact gttatttggc
     6601 cctctgagcg acaaaatagg gcgtaaacgt ccgttaatct tgtcgttgct attgtttatt
     6661 gtttcttcca ttttatgcgc gacaacgaac aatatttact ggctggtggt ctggcgtttt
     6721 attcaaggga tcgcgggggc gggtggttcg gtgctctctc gttctattgc tcgtgacaaa
     6781 tatcagggag taacgttgac ccagtttttt gcgctgttaa tgacggtgaa tggcctggca
     6841 ccggtgttgt cgccagtgct gggcgggtac attgttagca cttttgactg gcgcacttta
     6901 ttctgggtaa tggctgaaat tagcaccgta ctgttgctgg gctgcctgtt atttattaat
     6961 gagactttgc cagaaaataa aaggggctca tcattgctat taaccggacg aagcgtggtg
     7021 cagaaccgcc gttttatgcg cttttgcctg attcaaagct ttatgctggc tggtttgttt
     7081 gcatatatcg gctcttcgtc gttcgtgttg cagaaggaat ttggctttag tccaatgcaa
     7141 tttagcctgg tgtttggcct taacggcatc ggacttatca ttgcttcatg gatcttctcg
     7201 cgcctggcgc gacggattaa cgcgatgaca ttgttgcgag gtggcctgat agcggcaatt
     7261 ttgtgtgcat tgctcacggt cttatgcgca tgggtacaat tgcccattcc ggcactggtg
     7321 gcattatttt tcaccatcgc attttgtagc ggcatcggca ctgttggcgg ggcagaggct
     7381 atgagtgcag tagggacgca ggaatctgga acggcgtctg cgttgatggg gatgagcatg
     7441 tttgtcttcg gcggtatagc cgcgccattg tcgggaattg gcggagaaac actgttaaaa
     7501 atgagtctgg caattacggt gtgttatacg ctggcattgc tggttgctct caccagaatc
     7561 gacaaccaaa agtaatgaac acctcatcct gaggggggcc tgtcaggatg aggtgcaaaa
     7621 cactaacggt ccttacgcca cgcatccgcc gtcaatgctt cgccaaaatg accggcgatc
     7681 agccgtttgg tgagttcatg cagcggcgat gccagcacat ctgcggtgct gcctcgctcg
     7741 acaacctcgc cctgatgcat caccagcacc tggtcgctaa tgtgcttcat cattccgata
     7801 tgctgggtaa cataaatata cgaaatgccc tgtttttcct gtaactccag catcagatta
     7861 atcaactgcg aacgcatcga catatccagt gaggcgaggg cttcatcggc aataatgact
     7921 tttgggcgca atatcagcgc gcgcgccaga cccagacgct gtttttgccc gggtgctaac
     7981 atatgcggat agtaactgac gtgatccggt agcagcccaa ccatacgcat cgtttcaata
     8041 atctgtttgc gacgctgttc cggttccagg tcggtgttca ggcgcagtgg aaaatccaga
     8101 atttgcgaga tacgttgacg gggattcaac gaggtcgaag gatcctgaaa aatcatgcga
     8161 atacgctgac tgcggaagga ataatcgcca aaatgcagcg gatgatcgtc aatcaataac
     8221 tcgccgctgg taggttctat cattcccgcc agcatttttg ccagcgtgga tttacccgaa
     8281 ccattctcgc cgataattgc cagtgtctgg ccttcacgta gcgtaaagct caagggtttt
     8341 accgcttcta cggtctgacg acgaaaccag ccggtccggt agcggaacgt tttacttaaa
     8401 ttgcgcactt caagtagcgt ttcgatcatc tcactctttc tccatgttca gcgggaaatg
     8461 acaggcatag agatgatttt tcgcccccgt caaacgtggc gtcacaatgc attctcgttg
     8521 tgcatacggg caacgtggcc ccagacgaca accaatcggt aactgttcca gcagcgggat
     8581 agcgccgggc agcgtattga ggcgactttt atgcggcatc gcgctgccga agtctggtat
     8641 cgcgcggatc agcgcctggg tataaggatg gtgtggcatc gtcaccaact ccttactcgg
     8701 cgcggtttcc accgtttgac cgcagtaaag cacgttaatt ttatccgccc attggctaag
     8761 catttgtaag tcatggctga taagcaaaat agtggtattg ctgttttggt tgagacgcgt
     8821 cagcaggcga aagatttgcg cctgggttgt tggctccatt gagttggtcg gttcgtcagc
     8881 aattagcaga cgcggttgat tcgccagtgc aatggctatc atcactttct gacattcacc
     8941 ttcggtcaac tcatagggaa aactgcgcat cgcatctttg tgatctttaa tccccacgcg
     9001 gtgcagcagt tcaatggcac ggcgtttgcg ccagccaaaa cgctgccacc aacggccttt
     9061 ataggtccag gcagggatgt tttgcatcaa ctggcgaccc acacgttctg aagggtcaag
     9121 acacgactgt ggttcctgga aaatcatcga cacgttgtga ccaaccagct tgcgccgttc
     9181 gcgtgcggag agacgcagca aatcgatatc atcaaaacgc atacggtcag cagtaacacg
     9241 ccagttatct ttattcaccc cacaaatcgc tttagcaatc aaacttttgc cggaaccgga
     9301 ttcgccaaca agaccgcgga tttcaccttc ggttaaggtc atacttacgc ggtcgacggc
     9361 tttaacccac tcatcaccgg ttttaaattc aatggtcagg ttacgaatat cgagtaatgg
     9421 cattattcca cccccgcaat aattgcacga cggacaccgt cgccaaggag gttaaccaac
     9481 agcacgctaa tcataattgc cgcacctggc agcatgacag tccatggggc gacataaatc
     9541 agttccagcg catcaccgag catcgcgccc cattcaggcg aggggagttg tgcgccgagg
     9601 tcaagaaagc ccagcgcggc gatatcgaga attgccatcg acagtgcgcg ggtgatctcg
     9661 gtgaccagcc cggcggtgat gttaggcatc acagcaaacc agagaatatt cagcgttgat
     9721 gcgccatcca gacgggcggc gataacgtac tctttttcca gttcgtcatg caccatgctg
     9781 taaatcgaac gtaccatacg cggcagcagc gccagccaga cagcaaacat ggcgtgagac
     9841 aaactcggtc cggcaaacgc tacaacgata attgccagca gcagcgaagg gatcgccagt
     9901 aaggtatcca gaatatggtt aagcaccgct gagcgtaggc cgtgggtcgc cccggcgaag
     9961 gtgcccagta ccaggccaca gatcgtcgcg gcgagcgtca ccacaaatgc gccacctacg
    10021 gttggcgcag ctccgctcag taaacggctt aaaacatcgc gtccgaggtc gtcagtcccc
    10081 aggaagaaag aaacttcgcc atagcgcgac catgacggcg gcaataattg ataaccgaga
    10141 aattgctggt cgatgccgta aggcgcaaac cagccgccaa aaatacacag tacagccagc
    10201 cccgcgcagc cgtacaggcc gaccatcgca gaggcgtcac tataaaattt gcgccaggcg
    10261 gtacgcagcg tgcccggcgg gcgcttttcg ctgtatacgc tatcgtaagg cataccattc
    10321 cttatgtttc agagggttag ccatggcacc caaaatatca gaaatcacgt taacaataat
    10381 gaccagtgag ccacacacca tcactccggc ggaaatggct gcgtaatcct gctggcggat
    10441 ggcgttaatt aaccagcgcc ctaaacccgg ccagctaaaa accatttcgg tgatcatcgc
    10501 cagcgtcagc atggtagaaa actgtaagcc caggcgagga ataaccggag gtaacgcgtt
    10561 atgcagaacg tggcgacgca aaatagtaaa gcgcgacaaa ccgcgggtcg ccgctgcttt
    10621 aacatagttc tggtcataca cttcgatagt gctgatacgc atcagtcgga tcacttcagt
    10681 tgttggcgca accgacaggg tgatcacggg caataccata tgacggattg cgctaatgat
    10741 catttcatcc cgccacggtg agtccgaaag ccaggcatca atcaacgcaa aaccggtaat
    10801 cggtttcact tcgtagagca aatcgaaacg cccagaaacg ggcaaccaac ctagtgtgag
    10861 tgaacaaaac agcgtcagca gaagcgccag ccagaacacc gggattgaaa agcccagtaa
    10921 ggcgatggcg ttgatcaagt tatcctgcca cttatggcgc gtaatcccgg caatcattcc
    10981 tacggggatg ccaacaatca gcgcaaaacc gaaggcgagg atgcacagtt ccatcgtcgc
    11041 cgggaagacc tcttttaact gttcggcaat cggttgaccg ttaatgctgg aaacaccaaa
    11101 atcccagtgg atcaggccgt taaaccagaa cacccaggca ttccacagtg acgcgccttg
    11161 taacggcgca tgtggggtga aatagctcaa gctgaagcca acgaaggtca gcaggaacaa
    11221 ggtcacaatc aataacaaaa tacggcgtaa ggtgaagata atcatggctt tttcacctca
    11281 tcctgtttct cgcgatacac cccagcaaag gaggcgttac caaacgggct aagtaccaga
    11341 cctttgatat cgtaccgata ggcctgcaaa cgcaatgacg acgccagcgg cagaataggt
    11401 aattcctgcg ccagaatact ttgcgcttcg tcataggctt caatacgcgc cgccagctgc
    11461 tgcgaagaga gcgccttacg caatacgctg tcgaatttcg gatcgcacca gtgggcgagg
    11521 ttggtctgcg aatgaattgc tgcgcagctc agtaacggac ggaagaaact gtccgggtcg
    11581 ttactgtccg tcgcccaacc ggataacgtc agatcatgac tcatatccat caaccgtgcc
    11641 tcctgaaaac gaccttctac cggcacaatc accactttta cgccaacctg tgccatatcc
    11701 gcctgaatca gttcggcggt tttcagtgga ctggggttcc acgcctgcga acgtgtgggc
    11761 acccacagtt tcagcgttaa gttttccagc cccaacgcct tcaactgttc gcgcgatttt
    11821 gccggattgt attcagtaat tttagcctcg ttgtcatagg cccacgaggc tcgcggtaaa
    11881 atagaggccg ccgtttcagc cgtaccataa tagatggatt gcatcaggcg ctggttatta
    11941 attgccagtg ccagcgcatg gcggacagcg ggattattta gcggcggttt agcggtgtta
    12001 aacgccagat aggcgacgtt catccccggg cgcagcgtta aacgcaagcg tgggtcgtca
    12061 cgcaaaatgg atagctgact ggcagcaggc caggccagaa cgtcgcactc cccggtcagg
    12121 agtttcgaca atcgtcctgt accgccagag cctaaatcca ccaccacttg tggcatcaac
    12181 ggcttaccac gccagaaatc atcatgacgt tgtaagcgaa tatattgccc ggcgcggtat
    12241 tccgacaact gatacggacc ggtgccgacc ggttgacggt cgagttgctc ctggcgatct
    12301 tctttctcta acttccctgc atattctgcc gacatgaccg aagcataatg ggtcgccagg
    12361 tgccacaaaa aagaagcatc cggctgggcg agacggaact caacggtatg attatccagc
    12421 ttgcggacgc ttttcacgtt atcggcaaat tgcagactgt cgaaataggg gaagttactg
    12481 ccgttgacgt tatgccacgg gttgttgcgg tcaaaaatcc gttggaaggt aaacaccaca
    12541 tcatcggcat tcattttacg agtgggagta aaccagtcgg ttttttgaaa cggaacatcg
    12601 cgacgcaggt ggaagcgata ggtcgcgccg ttgtcgagca cttcccagct ttcggcaagt
    12661 tccggcatca ggcgataggt atagggatcg acatccagca gtcgatcata aaactgggcg
    12721 gcaagggtat cgacaattaa cccactgctc gctttggatg ggttaaaggt gttgacttgc
    12781 ccgctgacgc aatagacaaa accgctgtca cggatatcag catgcggggg ggattcaggc
    12841 gcggcgattg cctgaccact cacaagtcca gcaatcacca aaagagacga taatacctgg
    12901 cgcataatat taagggattt tatgtaaaga ggctatctta ctaatattta atgacatttg
    12961 ccattaccgt ttgttttcag aggtcgtaat gagggccgtg tggttggtca ttgaacaggt
    13021 ggcgtcgcca tcagttcaac attcgtaccc gtgatttctt tataactata actcacagaa
    13081 caacttagcg aggagcagag cggtacgatc gtgagaaacc cattaaagca cgctgctgtg
    13141 cgtgtcattt ttagccggat gcggcgcgaa cgctttatcc gggcaacgat gctgaccgat
    13201 agtctgcaat taaataatta ctcattaccc cattgattca aaaactctgc gatctcatca
    13261 atgcgtacgg gattaatccc cgcttcagca gccatttcat gttgggcttc ttcactgatc
    13321 tcttcattat tcatcaaacg ggtgagcagt aactggaagt agtgggccag cggtgtgcgt
    13381 tcagcgtctg cgacaggctc tgcgcattcg gtcgcgtact catcggcaat atcaaaatat
    13441 tttagcggga tatcgtggtt cattatttgc ccctggggtt aatgcgctaa gcataagggc
    13501 ggatgatagc actcgtgatc cgggtcatac atctttttat agttaatggc gaggtgcatt
    13561 accctcaggc aggataatgc gccgcgcatt cgactattac gtttcgttca ccggcgttct
    13621 gtcgctatca tacaacggca ctttgcgata gcggcggatc aaataccaca aatatgcgcc
    13681 gcctaacgaa gcccatacca tcccggttgc gcaattcgat gcggcaatga aaattttgca
    13741 ggatgcgctg agagattaat acgcagaagc agggttaacg caataatgcc tgatgcgcag
    13801 cgcttatcag gcctgcaaac gacgtattga ttatgtatgc cggataaagc gttcacgctg
    13861 catccggcaa gttgtattgc ccaacttcgc taaatctggt gctttttcaa caacgcgcgg
    13921 aactgatgat aggttaaccc cagcaattca gcggcgcgct tctggttata cttcccttgt
    13981 tccaagctga cctgaagcag ttctttttcc tgctgcatct gaaactcgcg taaatccagt
    14041 ggcagcgtcg gaagcgaagt gttttctgaa acggcgatgg cttcttcaga tggacggcgt
    14101 ttaaagggat caataatgat gtcatcaagc ggataatcgc tggtgccgtg acgatacact
    14161 gaacgttcca ccacgttttt caattcacgt atatttcccg gccagcgata attcagtaat
    14221 gtttctctgg cgtgctcggt aaaacccggg aacagaggca gcttgatttc ccgacacatc
    14281 tggatggcaa agtgttctgc catcaacata atgtcgcttt cacgctcgcg cagtggtggc
    14341 agttgcacaa catcgaacgc tagtcggtcg agcaggtcag cgcgaaaagt accttcattg
    14401 accatcgccg ggagatcggc attcgtcgcg cataccaacc gcacattcac ctgcaatggt
    14461 tggctgccgc cgacgcgctc cagttcaccg tactcaatca cacgcaataa tttttcctgc
    14521 accatcatcg gtgccgtagc gagttcatca agaaatagcg taccgccgtc ggcacgttca
    14581 aatctccctg gatgacgttt ttgcgcaccg gtaaacgccc ccgcttcatg accaaacagt
    14641 tcggaatcca gcaaattttc atttaacgcc gcgcagttaa gggaaataaa cggcccttgc
    14701 caacgggagg agagataatg caggcggctg gcgatcagct ctttaccggt gccgcgctcg
    14761 ccgatgatga gcaccggttt gtccagcggt gcgagatgcg aaacctgttc cagcacttcg
    14821 agaaagctgt tcgcctcacc gagtaaatta tctttgtatt ctgccatgat gaaattcgcc
    14881 acttgttagt gtaattcgct aattcatcct ggcatgttgc tgttgactct tcaatcagat
    14941 ctttataaat caaaaagata aaaattggca cgcaaattgt attaacagtt cagcaggaca
    15001 atcctgaacg cagaaatcaa gaggacaaca ttatgggtat tttttctcgc tttgccgaca
    15061 tcgtgaatgc caacatcaac gctctgttag agaaagcgga agatccacag aaactggtgc
    15121 gtctgatgat ccaggagatg gaagatacat tggttgaagt acgttctact tcggcgcgtg
    15181 cgctggcaga aaagaaacag ctgactcgtc gtattgaaca agcgtcggcg cgtgaaattg
    15241 aatggcagga aaaagcggaa ctggcactgc tgaaagatag agaagatctg gcgcgtgcgg
    15301 cgttaattga aaaacagaaa ctgaccgatc tgattaagag cctggaacat gaagtgacgc
    15361 tggtggacga tacgctagtg cgtatgaaga aagagatcgg cgagctggaa aacaaattga
    15421 gtgaaacacg cgctcgccag caggcattga tgttacgcca tcaggcggca aactcgtcgc
    15481 gcgatgtgcg tcgtcagctg gacagtggca aactggatga agcaatggct cgtttcgaat
    15541 ctttcgaacg tcgaattgac cagatggaag cggaagcaga aagccacagc ttcggtaaac
    15601 aaaaatcgct ggacgatcag tttgccgaac tgaaagccga tgatgcaatc agcgagcaac
    15661 tggcacaatt aaaagccaaa atgaagcaag acaatcaata ataacatcca ggcggcgtcc
    15721 gaacgcgccg ccgctcatcg tctaaggagt ccttatgagc gcgctatttc tggctattcc
    15781 gttaaccatt tttgtgctgt ttgttttacc gatctggtta tggctgcatt acagcaatcg
    15841 ttctggtcgc agtgaattgt cgcaaagtga gcagcagcga ttagcgcaac tggctgatga
    15901 agcaaaacgg atgcgcgaac gtattcaggc gctggaatct attcttgatg cagaacatcc
    15961 gaactggagg gatcgctaat ggcgggcatt aatctcaata aaaaattatg gcgtattccg
    16021 cagcagggca tggtccgtgg cgtctgcgcc gggattgcca actattttga tgtaccagta
    16081 aaactggtgc gtatcctggt ggtgctgtcg attttcttcg gtctggcgct gtttaccctg
    16141 gttgcttaca tcattttgtc atttgcgctt gatccaatgc cggacaacat ggcctttggt
    16201 gagcagctac cttccagccg cgaattgctg gatgaagtcg accgtgaact ggcggcaagt
    16261 gaaacgcgtt tacgcgagat ggaacgttat gtcacttccg atactttcac gttacgtagc
    16321 cgtttccgtc aactgtgagg aaagttatga atactcgctg gcaacaggcc gggcaaaagg
    16381 tgaagcctgg gttcaaatta gcaggcaagc tggtacttct taccgcactg cgctatggcc
    16441 cggcgggtgt ggcgggctgg gcgataaaat cagttgctcg ccgaccgctg aaaatgttgc
    16501 tggctgtggc gctggaaccg ctgttaagtc gggctgccaa taaactggcg cagcgttata
    16561 aaaggtgagg ggagtttcgc aaaaatggtt aaatctcagg cgtataatgg atagcaattt
    16621 tcatccatag aaggacgctt acatgtttaa aaaaggctta cttgctctga cactggtgtt
    16681 ttcactgccc gttttcgccg ctgaacactg gatcgatgtt cgtgttccag agcagtatca
    16741 gcaagaacac gttcaggggg ccatcaatat tcccttgaag gaagtgaaag agcgcattgc
    16801 caccgccgtt ccggataaaa acgacaccgt taaagtgtat tgcaatgccg gacgccagtc
    16861 agggcaggca aaagagatcc ttagcgagat gggatatacc cacgttgaga acgccggtgg
    16921 cctgaaagac atcgcaatgc cgaaggtcaa aggttaaaag aattcaccat gagcggcgct
    16981 tatgcgccgt tttttctgtc acaccctatt tacatcccca tagattattt gcgttagctc
    17041 acaaatacgc tttttcccag gtaaaaaaag atttcctgcg tgactaaacc ccttgagctc
    17101 aattgacagt ttattttatg cggagtagtc tctcgtttca tgggaccgct accacggaaa
    17161 ggcaacatga aacagaaaat tacggattac ctggacgaaa tctacggtgg aacatttacc
    17221 gcgactcatt tacagaaatt tgtaacgcgt cttgagagtg cgaaacgatt aattacacag
    17281 cgacgtaaaa aacactggga tgaaagtgat gtcgtgttaa ttacctatgc cgatcaattt
    17341 cacagtaatg atttaaagcc actacccaca tttaatcagt tttaccctca atggctgcaa
    17401 agaatttttt cacatgttca tttattacct ttttatccat ggtcatctga tgatggattt
    17461 tcggtaattg attatcatca ggtcgccagt gaagcggggg agtggcagga tattcagcaa
    17521 ctcggtgaat gcagccattt aatgtttgat tttgtctgca accatatgtc ggcaaaaagt
    17581 gaatggttta aaaactattt acaacagcag ccaggttttg aagatttttt tattgccgtt
    17641 gatccgaaaa ccgatctcag cgccgtcact cgcccgcgtg cgttaccgtt attaacgcca
    17701 ttccagatgc gcgatcattc aatgcgccat ttatggacca cctttagcga cgatcaaatt
    17761 gacctcaatt accgtagccc tgaagtgttg ctggcgatgg tggatgtatt gctgtgctac
    17821 ctggaaaagg gcgcagagta tgtccgtctg gatgccgttg gctttatgtg gaaagagccg
    17881 ggaacaaact gcatccatct ggaaaaaaca catctgatta tcaaactgtt acggtcgatt
    17941 attgataacg tcgcgccagg cacagtgatc attaccgaga ccaatgttcc gcataaagac
    18001 aacattgctt actttggcga aggcgatgac gaagcgcata tggtgtacca gttctcgctg
    18061 ccgccgctgg tgctgcatgc ggtgcaaaaa cagaacgttg aggcactttg tgcgtgggcg
    18121 caaaacctga cactaccttc cagcaacacc acctggttta acttccttgc ctctcacgat
    18181 ggcatcgggc ttaacccact gcgggggttg ttgcccgaaa acgaaatatt agcgctggtc
    18241 gaggtgttac agcaggaagg agcattagta aactggaaaa ataatcccga cggtacgcgt
    18301 agcccctatg aaatgaatgt cacctatatg gatgcgttga gccgccgtga gtgtagcgac
    18361 gaagaacggt gtgccaggtt tattctcgcc catgcgattt tgttaagttt ccccggtgtg
    18421 ccagcgatat atattcaaag cattctgggg tcgcgtaatg attacgcagg tgtcgaaaaa
    18481 cttggatata atcgtgcgat taaccgtaaa aaatactcta gtaaagagat aacgacagaa
    18541 ctgaacaata aagcgacatt aagttattcg gtatattata agttgtcgca tttaattaca
    18601 cttcgtcgca gtcataaaga gtttcatccg gataataatt tcaccattga tgcgattaat
    18661 tcatttgtaa tgcgaattca aagaagcaat gcggatggcg attgcctgac gggattgttt
    18721 aatgtcagtg aaaatattca gcatataaat attactgacc tgcacggtcg ggatctgatt
    18781 agtgaagttg atatattggg taatgaaata acactgcatc cctggcaggt tatgtggatt
    18841 aaataaaaag gaacatttca tgattaaatc aaaaatcgtg ctgttatcag cactggtttc
    18901 ctgcaccctg atttcaggct gtaaagaaga aaataaaaat aatgtatcca tcgaatttat
    18961 gcattcttcg gtggagcagg agcgccaggc cgtcatcagc aaattgattg cccgttttga
    19021 aaaagaaaac ccaggtatca ccgtaaaaca agtgcccgtg gaagaagacg cctataacac
    19081 caaagtcatt accctttcac gtagcggttc gctgccggaa gtgatcgaaa ccagccatga
    19141 ctacgccaaa gtgatggaca aagagcagct tatcgatcgc caggcggttg ccacggtcat
    19201 cagcaacgtc ggtgaaggcg cgttctacga tggcgtactg cggattgtgc gcaccgaaga
    19261 tggcagcgcc tggaccggtg ttcctgtcag cgcatggatt ggcgggatct ggtatcgcaa
    19321 agatgtgctg gcaaaagcta ggctggagga gccaaaaaac tggcagcaat tgctggacgt
    19381 tgcacagaaa ctgaatgacc cggcgaataa aaaatacggt attgcgctgc ctacagcaga
    19441 aagcgtgttg acggaacagt ctttctccca gtttgcgtta tccaatcagg ctaacgtctt
    19501 taacgccgaa ggcaaaatca cccttgatac accagagatg atgcaggcgc tgacctatta
    19561 ccgcaacctt gctgccaaca ccatgccagg ttctaacgac atcatggaag tgaaagacgc
    19621 ttttatgaac ggcaccgcgc cgatggcgat ttactccacc tatatccttc cggctgtgat
    19681 taaagaaggc gatccgaaaa acgtcggttt cgtggtgcca accgagaaaa attctgcggt
    19741 ctacggcatg ttgacctcgc tgaccattac cgccgggcaa aaggccgaag agacggaagc
    19801 agcagaaaaa tttgtcacct ttatggagca ggcggacaac attgccgact gggtgatgat
    19861 gtcgccaggc gcggcgctgc cggtgaacaa agcggtggtg actaccgcca cctggaaaga
    19921 caacgacgtt attaaggcgc tgggagaatt gccgaatcag ctaatcagcg aactgccaaa
    19981 tattcaggtc tttggcgcag taggggataa aaactttacc cgcatgggcg atgtgacggg
    20041 gtctggcgtg gtgagttcaa tggtgcataa cgtcaccgtg gggaaagccg atctccctgg
    20101 cacgctgcaa gcgagccaga aaaagctgga tgaactgatc gaacagcact aaacccagga
    20161 caggaatccg caatgaacag gcttttttca ggtcgttccg atatgccttt tgcactgctg
    20221 cttctcgcgc ccagcttatt actgctgggc ggtctggtgg cgtggccgat ggtgtcgaat
    20281 atcgaaatca gttttttacg tctgccgctc aatcccaaca tcgaggcaac atttgttggg
    20341 gtgagcaact atgtgcgtat tctctccgat ccgggcttct ggcattcgct gtggatgacg
    20401 gtctggtata ccgcgcttgt ggtggcgggc agcaccgtgc tcgggctggc cgtggcgatg
    20461 tttttcaacc gcgagttccg cctgcgcaaa accgcgcgtt cgctggtgat cctttcctac
    20521 gtaacgccgt ctatttcgct ggtgttcgcc tggaaataca tgttcaacaa cggttacggc
    20581 attgtgaact acctcggcgt cgatcttctg catctctatg agcaggcacc gctgtggttc
    20641 gataacccgg gcagtagttt tgtgctggtg gtgctgttcg ctatctggcg ctacttcccg
    20701 tatgccttta tctcgtttct ggcgattttg cagaccatcg acaaatcgct gtacgaagcg
    20761 gcagagatgg atggcgcgaa tgcctggcag cggtttcgca tcgtcacgct gcccgcgatt
    20821 atgccggtcc tggcgacggt ggtgaccctg cgcactatct ggatgttcta catgttcgcg
    20881 gatgtttatc tgctgacgac caaggtcgat attctcggtg tctatctcta caaaaccgcc
    20941 tttgccttta atgacttagg gaaagcggcg gcgatctcgg tggtgctttt catcattatt
    21001 ttcgctgtca ttctgctgac caggaaacgg gtgaacctca atggcaacaa ataaacgcac
    21061 actcagtcga atcggttttt actgcgggct ggcgctgttt ctcatcatca cgctgtttcc
    21121 attttttgtg atgctgatga cctcgttcaa gagcgcaaaa gaggcgatct cgctgcatcc
    21181 tacgctgctg ccacagcagt ggacgctgga gcattacgtc gacattttta atccggtgat
    21241 tttcccgttt gtcgactact tccgtaacag catggtggtg tcggtggttt catccgtggt
    21301 ggcggtattt ctcggcattc tcggggctta cgcgctttcc cgcctgcgct ttaaagggcg
    21361 gatgaccatc aacgccagct tttacacggt gtacatgttc tccggcattt tgctggtggt
    21421 gccgctgttc aaaatcatca ccgcacttgg catttacgac accgagatgg cgctgatcat
    21481 taccatggtg acgcaaaccc tgcccaccgc cgtgtttatg ctgaaaagct acttcgacac
    21541 catccctgat gagatcgaag aagcggcgat gatggatggc ctcaatcgtc tgcaaattat
    21601 cttccgtatc accgtgcccc tggcgatgtc cgggctgata tcggtgtttg tctactgctt
    21661 tatggtggcg tggaacgact atctgtttgc gtcgattttc ctctccagcg ccagcaattt
    21721 caccttacca gtgggcctga acgcgctgtt cagtacgcct gattacatct ggggacggat
    21781 gatggcggcc tcgctggtga ccgcattgcc agtggtgatt atgtatgcgc tttcggaaca
    21841 ttttattaag agtggtttga ccgccggtgg cgtgaagggc taaagcggcc agtttcttga
    21901 caaggagttt tacatgaaaa agttagtagc cacagcaccg cgcgttgctg cgctggttga
    21961 gtatgaagat cgggcgattt cagccaatga agtgaagatc cgcgtgcgat tcggcgcacc
    22021 gaagcatggg acggaagtgg tcgatttccg cgctgccagt ccgtttatcg atgaagactt
    22081 taacggcgaa tggcagatgt tcaccccgcg tccagcagat gcgccgcgcg gcattgagtt
    22141 tggcaaattc cagcttggca acatggtggt tggcgacatt atcgagtgcg gcagcgacgt
    22201 taccgcatac gcggtgggcg acagcgtatg cggctacggc ccgctttccg agacggtcat
    22261 cattaacgca gtgaataact acaagctgcg caaaatgccg caaggcagct cctggaaaaa
    22321 cgccgtctgc tacgacccgg cgcagtttgc catgagcggc gtgcgcgatg ccaatgtacg
    22381 cgtaggggat tttgtcgtgg tggtcgggct tggcgcgatc ggtcaaattg ccatccaact
    22441 ggcaaaacgc gctggagcgt cggtggtaat tggagtcgat cctattaccc gtcgttgtga
    22501 tattgcccgt cgccacggtg cggatttctg tcttaacccc attggcgctg acgtaggtaa
    22561 agagatcaaa acgctgaccg gcaagcaggg tgccgatgtg attatcgaaa ccagtggcta
    22621 tgccgacgcg ctgcaatcgg cactccgcgg tctggcctat ggcggcacca tctcctatgt
    22681 cgcgtttgcc aaaccgtttg ccgaaggttt taacctcgga cgcgaagccc atttcaataa
    22741 cgccaaaatt gttttctccc gtgcgtgcag cgagccgaac ccggattatc cgcgctggag
    22801 ccgtaagcgt attgaagaaa cctgctggga actgctgatg aacggttatc tcaattgcga
    22861 agatttaatc gatccggtgg tgacctttgc caacagcccg gaaagctaca tgcagtatgt
    22921 cgaccagcat ccggaacaga gcatcaaaat gggcgtcacg ttttaattca aaggaatgat
    22981 aaaaatgaaa atcggcacac agaatcaggc gttctttccg gaaaacattc tggagaaatt
    23041 tcgttatatc aaagagatgg gtttcgatgg ttttgagatc gacggcaaac tgctggtcaa
    23101 caatctcgaa gaagtcaaag cagcaatcaa agaaaccggt ttaccggtga ccaccgcctg
    23161 cggtggctat gacggttgga ttggcgactt tatcgaagag cgtcgactta atggcttaaa
    23221 gcagatcgaa cgcattcttg aagctctggc agaagtgggc ggtaaaggta tcgtcgttcc
    23281 ggctgcgtgg ggcatgttta ccttccgctt accgccgatg acctcgccgc gtagcctgga
    23341 cggcgaccgc aaaatggtga gtgattccct gcgcgtactg gagcaggtgg cagcgcgtac
    23401 cggaaccgtg gtgtacctcg aaccgttaaa ccgctatcag gatcacatga tcaacaccct
    23461 cgccgatgct cgtcgttaca tcgtcgaaaa cgatcttaaa catgtacaga ttatcggcga
    23521 tttctatcac atgaatatcg aagaagataa cctggcgcag gcgttgcacg acaaccgcga
    23581 cttgctcggt catgtgcata ttgccgataa ccatcgctac cagccgggca gcggcaccct
    23641 cgatttccac gcgctgtttg aacagctgcg cgccgataac tatcagggct atgtggtgta
    23701 tgaagggcgt atccgggcag aaaatcctgc ccaggcgtac cgtgattctt tggcttggtt
    23761 gcgtacctgc taagaggtct ttgtgaaaag tgcaatgaca agctctccac tgcgggtcgc
    23821 gataatcggc gcaggccagg tggcggataa ggttcatgct tcgtactact gcacccgcaa
    23881 tgatctggaa ctggtggctg tctgtgacag ccgcctttcc caggcgcagg cgctggcaga
    23941 aaaatacggg aatgcatccg tgtgggacga tccgcaggcc atgctgctgg cggtgaaacc
    24001 tgatgtggtt agcgtctgct cacctaaccg ttttcattac gaacataccc tgatggcgct
    24061 ggaagcgggc tgccatgtga tgtgcgaaaa accgcccgcc atgacggcag aacaggcgcg
    24121 ggaaatgtgc gataccgcgc gcaaacaggg caaggtgctg gcctacgact ttcaccatcg
    24181 ttttgcgctc gatacgcaac agctgcgtga acaggtgacc aacggcgttt tgggagagat
    24241 ttacgttacc accgcccgcg ccctgcgtcg ctgcggcgtt cccggctggg gtgtctttac
    24301 caataaagaa ctgcagggtg gtggcccgct gatcgacatc ggcattcata tgctggatgc
    24361 ggcgatgtat gtgctgggct ttccggcggt gaaaagcgtg aatgcgcata gttttcaaaa
    24421 gatcggcacg caaaagagct gtggtcaatt tggcgagtgg gatccggcaa cttatagcgt
    24481 cgaagattcg ctgtttggca ccattgaatt tcataacggc ggcattctgt ggctggaaac
    24541 gtcatttgca ctcaacatcc gcgaacagtc gattatgaac gtcagctttt gtggtgataa
    24601 agccggtgcg acgctgtttc cggcacatat ctacaccgat aacaacggtg aattaatgac
    24661 gctgatgcaa cgggaaatgg cagacgacaa ccgccatttg cgcagcatgg aagcctttat
    24721 taatcacgta cagggcaagc ccgtgatgat agccgacgcc gagcaggggt acatcatcca
    24781 gcaactggtg gcggcgttgt atcaatccgc agaaacaggg acgcgtgtgg aattatgacc
    24841 aggccagtaa cgttatcaga accccatttc agccagcata cactgaacaa atatgcttca
    24901 ctgatggcgc aggggaacgg ctatcttggg cttcgcgcca gccatgaaga agattacacg
    24961 cgccagacgc gcggcatgta tctgacgggg ctgtatcatc gggcgggtaa gggtgaaatc
    25021 aacaaactgg tgaacctgcc tgatgtcgtg gggatggaga ttgccataaa tggtgagatt
    25081 atctcgttat cccgcgaagc atggcagcgt gagcttaact ttgccagtgg cgaattacgt
    25141 cgcaacgttg tctggcgtac cagcaacggc actggttaca ccatcgccag ccgtcgcttt
    25201 gtttcggcag accaactgcc gctgatcgcg ctggaaatca gtattacccc gctggacgcc
    25261 aacgcgtcag tgctgatttc aacaggcatt gacgccacgc aaaccaatca cggtcgccag
    25321 catctcgacg aaacccaggt gcgggtgttt ggtcagcatc tgatgcaggg gatctacacc
    25381 acccaggatg ggcgcagtga tgtggccatc agctgttgct gtaaggtgag cggtgatgtg
    25441 cagcaatgct ataccgccaa agagcgccgt ttactgcaac atacctgcgc gcagcttcac
    25501 gcaggtgaga cgttgacgct gcaaaaactg gtgtggatcg actggcggga cgacagacaa
    25561 gccgctttag acgaatgggg cagtgcgtcg cttcgtcagc ttgaaatgtg cgcgcagcag
    25621 agttacgacc aacttcttgc agcatcaaca gaaaactggc gtcaatggtg gcagaaacgt
    25681 cgtatcacgg taaacggcgg cgatgcgcac gatcagcaag cgttagatta tgcgctttat
    25741 cacctgcgca tcatgacgcc tgcccacgat gagcgcagca gcattgcggc gaaaggttta
    25801 accggcgaag gctacaaagg ccacgttttc tgggatacag aagtattttt gctaccgttc
    25861 catctgttta gcgatccgac ggttgcccga agtttactgc gttatcgctg gcacaacttg
    25921 cctggcgcgc aggagaaagc gcgacgcaac ggctggcagg gcgctctgtt tccgtgggaa
    25981 agcgcgcgta gcggcgaaga agagacgccg gaatttgctg ccattaacat tcgtaccggg
    26041 ttgcggcaaa aagtggcctc tgcgcaggcg gaacatcatc tggtggccga tatcgcctgg
    26101 gcggttattc aatactggcg gaccacgggg gatgaaagtt tcattgctca tgaaggcatg
    26161 gcgctacttc tggagacggc aaagttctgg attagccgcg cggtgagggt taacgaccgt
    26221 ctggaaattc atgatgttat tgggccagat gaatataccg aacatgtcaa taataacgcc
    26281 ttcaccagct atatggcgta ttacaacgtc cagcaggcgc tgaatatcgc tcgtcagttt
    26341 ggctgtagcg acgacgcgtt tattcatcgt gcagaaatgt acctcaaaga actgctgctg
    26401 ccagaaattc agcccgacgg cgttttgccg caggatgatt cgtttatggc taagccggtg
    26461 attaatctgg cgaaatacaa agcggcggcg gggaagcaaa ccattctgct ggattattca
    26521 cgcgcagaag tgaacgagat gcagatcctc aaacaagctg atgtggtgat gctcaattac
    26581 atgctgccgg agcagttctc agcggcatcg tgtcttgcca atctgcaatt ttatgaaccg
    26641 cgcactattc acgactcgtc attaagtaaa gcaatccacg gcattgttgc cgcacgctgt
    26701 ggcctgctga cccaaagtta tcagttctgg cgcgagggga ctgaaatcga tcttggtgct
    26761 gatccacata gctgtgatga cggtatccac gctgccgcaa ctggcgctat ctggctgggg
    26821 gcgattcagg gttttgccgg ggtgagcgtg cgtgacggtg aattacatct caatccggcg
    26881 ttacctgagc agtggcaaca gttgtctttc cctctgttct ggcagggctg cgaattacag
    26941 gtcactctcg acgcgcagcg tattgcgatt cgaacttctg cgcccgtttc actgcgcttg
    27001 aacggtcagc ttatttacgt cgctgaagaa tctgttttct gtttggggga ttttattttg
    27061 cccttcaatg ggaccgctac cacgcatcag gagggtgaat gaaactgcaa ggggtaattt
    27121 tcgatctgga tggagtaatc acagatagcg cacatctgca tttccaggcg tggcagcaga
    27181 ttgccgctga aattggcatc agcattgatg cgcagtttaa cgaattccta aaagggatca
    27241 gccgcgatga gtccctgcgg cgaattctgc agcacggggg taaagagggc gattttaacc
    27301 cacaggaacg ggcgcaactg gcgtatcgca aaaatctgct ttacgtccac tcactgcgcg
    27361 aactgacggt taatgctgtg ctgcctggca ttcacaattt gctggtagat ctccgagcac
    27421 agcagattcc ggttgggctg gcttctgtct ccctgaatgc gccgacgatt ttagcagcgc
    27481 tggagctgcg tgagtttttc accttctgcg cggacgcttc ccaacttaaa aactcgaaac
    27541 cggacccgga aatctttctc gccgcctgtg ccggactggg cgtgccgccg caggcatgta
    27601 tcggcattga agatgcgcag gcgggcattg acgccattaa cgccagcggt atgcgctcgg
    27661 tggggatcgg cgcaggctta accggggcgc aattattgtt gccctcaacg gaatcactca
    27721 cctggccgcg gttatcggcc ttctggcaaa acgtatagca aaggaatcaa catggctcag
    27781 ctttcgttac aacatattca aaagatctac gataaccagg tgcatgtggt gaaggacttc
    27841 aacctggaaa ttgccgataa agagttcatc gtatttgtcg gcccgtcggg ctgcggtaaa
    27901 tcgaccaccc tgcgtatgat tgccgggctt gaggagatca gcggcggtga tctgttgatc
    27961 gacggcaaac gaatgaatga cgttccagcc aaagcacgca atatcgcgat ggtgttccag
    28021 aactacgcgc tgtatccgca tatgacggtc tacgacaaca tggcgtttgg cctgaagatg
    28081 caaaaaatcg ccagagaggt gattgatgag cgggtgaact gggcggcgca aattctcggc
    28141 ctgcgtgagt acctgaaacg caagccgggc gcgctttccg gcgggcaacg tcagcgagtg
    28201 gcgcttgggc gggcgattgt gcgcgaagcg ggcgtgtttt taatggatga gccgctctct
    28261 aacctggatg ccaagctgcg cgtgcaaatg cgcgcagaga tcagcaagct gcatcagaaa
    28321 ctgaacacca ccatgatcta cgtgacccac gatcagaccg aagcgatgac catggcgacg
    28381 cggattggca ttatgaagga tgggattgtt cagcaggtcg gtgcgccgaa aacggtttat
    28441 aaccaacctg cgaatatgtt tgttgccgga tttattggat caccagcgat gaattttatt
    28501 cgcggcacga tcgatggcga taaattcgtt acggaaacgc ttaaattaac catacccgaa
    28561 gagaaattag cggttctgaa aacacaggaa agtttgcata agcccatcgt aatgggaata
    28621 cgcccggaag atattcatcc ggacgcgcaa gaggaaaata acatttccgc caaaattagc
    28681 gtggcggaat taaccggtgc ggaatttatg ctctacacca cggttggggg gcacgagtta
    28741 gtggtccgtg ctggtgcgtt aaatgattat catgcaggag aaaatatcac tattcatttt
    28801 gatatgacta aatgtcattt ctttgatgca gaaacggaaa tagcaatttg ctaaatacag
    28861 ggggaaggca ttctcccagg ataatacaag gaacaataat gaaaaagtta ttactctgta
    28921 ccgcactggt gatgtgtgcg ggaatggcct gcgcacaggc cgaggaaaag aacgactggc
    28981 actttaatat cggcgcgatg tacgaaatag aaaacgtcga gggttatggc gaagatatgg
    29041 atgggctggc ggagccttct gtctatttta atgccgccaa cgggccgtgg agaatttctc
    29101 tggcctatta tcaggaaggg ccggtagatt atagcgcggg taaacgtgga acgtggtttg
    29161 atcgcccgga gctggaggta cattatcagt tcctcgaaag cgatgatttc agtttcggtc
    29221 tgactggtgg tttccgtaat tatggttatc actacgttga tgaaccgggt aaagacacgg
    29281 cgaatatgca gcgctggaaa attgcgccag actgggatgt gaaactgact gacgatttac
    29341 gtttcaacgg ttggctgtcg atgtataaat ttgccaacga tctgaatacg accggttatg
    29401 ctgatacccg tgtcgaaacg gaaacgggtc tgcaatatac cttcaacgaa acggttgcct
    29461 tgcgagtgaa ctattatctc gagcgcgctt tcaatatgga tgatagccgc aataacggtg
    29521 agttttccac gcaagaaatt cgcgcctatt tgccgctgac gctcggcaac cactcggtga
    29581 cgccgtatac gcgcattggg ctggatcgct ggagtaactg ggactggcag gatgatattg
    29641 aacgtgaagg ccatgatttt aaccgtgtgg gtttatttta cggttatgat ttccagaatg
    29701 gtctttccgt ttcgctggaa tacgcgtttg agtggcagga tcacgacgaa ggcgacagcg
    29761 ataaattcca ttatgcgggt gttggggtga attactcgtt ctgataatgt gctaaattgc
    29821 cggatgcggg gcgagcgcca aatccggcat atatttattt caacgacata tgcgtagcaa
    29881 cggtaatatt ttgtggtgac ggttttccgg caattagctg gaataataac tcgcagcttt
    29941 gttgacctaa ctcctgcgtc ggaacatcga tgccgcctgg tgcaggcgtt aatataaacg
    30001 acagcgtttc attactataa cccaccaccg ctaactgctg cggaatagca atgtttttct
    30061 ctgctgctgc acgataaatg ctcattaatt tcaggctgtc agtggcaaat acggcttctg
    30121 gcaacggcga ctggcttaat aattgccgtg cggcttgcaa ggctgtttca tgggtataac
    30181 cgccgtcaac aatccattca tcacgcactg caatattatg cgcagccagg ctttgcttat
    30241 aaccatttac ccgatccact gaaacatgta catcaagcgg cgcatgcagg caggctatat
    30301 tcttatgccc gctttcaatt aacgcattgg tcagcgcaat actgtcgcca taattatcgg
    30361 tatcgacgga ataaacatgg ctgtattgac cttcaacttt accgatcacc accaccggga
    30421 tctcgtattt gtcgagttgt gcaaagaagg attcatcggc aggcgaactt agcataataa
    30481 tgcctttaat cattttctgc ttaattttgc tttcgcattt ttgcagatct tctgccggat
    30541 tgtgcgacgt ttgtaatatc acgtcgaaac cttcttcttc tgctttggcg gtgatggcat
    30601 gcaaaacttc cgagaaaaat ggattacccg ccgtagtttt ggtcgaacgg gtagaaatca
    30661 ccataatggc atcaaaaccc gaagaggtca gcgcgcgggc cagcttgttt ggttgatact
    30721 gtaattcttc aatggcccgt aacacttttt cgcgcgcttc cggggagata ttggtttgct
    30781 tattcagcac gcgtgatacg gtggattttg atacgcctgc aaccctggca atatcataaa
    30841 tagtagggga cataggtcag gaactccgtc cgaacgtgaa tgctgcacat cttatggagt
    30901 tcgtccgtcg atgacaacac gggtagttga taatcaatgg cctggccccc acattcatat
    30961 tcttacgaac gatttttttt ctggtcttca gagcagcaca ggacagcaat gaagcgactt
    31021 aaaaatgaac tcaatgcgct ggtgaatcgg ggtgtcgaca gacatctgcg cctcgctgta
    31081 accggactta gccgcagcgg caaaacagcg tttatcaccg cgatggttaa tcagttgctc
    31141 aatattcatg ccggagcacg tttgccgctt ttaagtgcgg tgcgtgaaga gcgcctgctg
    31201 ggcgtaaaac gcattcctca gcgtgacttt ggcattccgc gcttcaccta tgatgaagga
    31261 ctggcgcagt tatatggcga tcctcccgcc tggccgacgc caacgcgcgg cgtcagcgaa
    31321 attcgcctgg cgctacgcta taaatcgaac gattcgctgc tgcgccactt taaggatacc
    31381 tccacgctgt atctggagat tgtggattac cccggcgaat ggttgctcga cctgccgatg
    31441 ctggcgcagg actatttaag ctggtcgcgc cagatgacgg gcttactcaa tggtcagcgc
    31501 ggcgaatggt cggcgaaatg gcgaatgatg tgcgaagggc tggacccgct agcgcctgcc
    31561 gacgaaaacc gactggcaga cattgccgcc gcgtggaccg attatctcca ccactgcaaa
    31621 cagcaggggc tgcactttat tcagccagga cgctttgttt tgcctggcga tatggcagga
    31681 gcgcccgcgc tgcaattctt cccatggcca gatgtcgatg cctggggcga gtccaaactg
    31741 gcgcaggccg ataagcacac caatgccgga atgttgcgcg agcgttttaa ttattactgc
    31801 gagaaagtgg tgaaggggtt ctataagaat cacttcctgc gttttgaccg ccagattgtg
    31861 ctggtggact gcctgcaacc gctcaacagt ggaccacagg catttaatga tatgcgtctg
    31921 gcgctgacgc agctgatgca aagtttccac tacgggcagc gtaccctgtt ccggcgtttg
    31981 ttttcgccgg ttattgataa gctattgttt gctgccacta aagcggacca tgtgaccatc
    32041 gatcagcacg ccaatatggt ttcattgctg caacaactga ttcaggatgc ctggcaaaat
    32101 gcggcgttcg aagggatcag tatggactgc ctggggctgg cgtctgttca ggcgaccacc
    32161 agcggaatta ttgatgttaa cggtgagaaa atcccggcgc tgcgcggtaa tcgactcagc
    32221 gatggcgcac cgctcactgt ttatcctggc gaagttcccg cacgtttgcc tggtcaggcg
    32281 ttctgggata agcaaggctt ccagtttgag gcgtttcgtc cgcaggtgat ggatgtcgac
    32341 aaaccgctac cgcatattcg tcttgatgct gcgctggaat ttttaatagg agataaattg
    32401 cgatgaccga accgttaaaa ccacgtattg atttcgacgg tccactggag gtcgatcaga
    32461 atccaaaatt cagggcgcag cagacctttg acgaaaatca ggcgcaaaat tttgccccag
    32521 ccacgctcga tgaagcgcag gaagaagagg ggcaagttga agcggtaatg gatgcagcgt
    32581 tacgtccgaa acgcagcctg tggcgcaaaa tggtgatggg cgggctggct ctgtttggcg
    32641 caagcgttgt cgggcagggc gtgcagtgga caatgaacgc ctggcaaacc caggactggg
    32701 tggcgctggg tggatgtgcc gctggggcat tgattatcgg cgctggcgta ggttctgtgg
    32761 taacagagtg gcggcgctta tggcgcttgc gacagcgcgc ccatgaacgc gacgaagcgc
    32821 gcgatttgtt gcacagtcac ggcacgggta aaggccgtgc attctgcgaa aaactggcgc
    32881 agcaggcggg catcgatcag tcacatccgg cgctgcagcg ctggtatgcc tcaatccatg
    32941 aaacgcagaa cgatcgtgaa gtggtcagcc tgtatgctca cctggtccag ccagttttag
    33001 atgcccaggc gcggcgcgaa atcagccgtt cggcagcgga atcaacgtta atgattgccg
    33061 ttagcccact ggcgctggtg gatatggcgt ttatcgcctg gcgcaatctg cgtttgatta
    33121 atcgcatcgc cacgctgtat ggcattgaac tgggatatta cagccgtttg cgcctgttta
    33181 agctggtatt gctgaatatt gcttttgccg gagccagcga actggtgcgc gaagtgggga
    33241 tggactggat gtcgcaagat ctcgctgcgc gtttgtctac ccgggcagct caggggattg
    33301 gtgctggact tctgacggca cgactgggaa ttaaagctat ggagctttgc cgcccgctgc
    33361 cgtggattga cgatgacaaa cctcgcctcg gggatttccg tcgtcagctt atcggtcagg
    33421 tgaaagagac gctgcaaaaa ggcaaaacgc ccagcgaaaa ataatgcaat atcgggtgct
    33481 gaccggatat ctttacgccg aagtgcccgt ttttccgtct ttgtgtcaat gattgttgac
    33541 agaaaccttc ctgctatcca aatagtgtca tatcatcata ttaattgttc ttttttcagg
    33601 tgaaggttcc catgcgtctg gaagtctttt gtgaagaccg actcggtctg acccgcgaat
    33661 tactcgatct actcgtgcta agaggcattg atttacgcgg tattgagatt gaccccattg
    33721 ggcgaatcta cctcaatttt gctgaactgg agtttgagag tttcagcagt ctgatggccg
    33781 aaatacgccg tattgcaggt gttaccgatg tgcgtactgt cccgtggatg ccttccgaac
    33841 gtgagcatct ggcgttgagc gcgttactgg aggcgttgcc tgaacctgtg ctctctgtcg
    33901 atatgaaaag caaagtggat atggcgaacc cggcgagctg tcagcttttt gggcaaaaat
    33961 tggatcgcct gcgcaaccat accgccgcgc aattgattaa cggctttaat tttttacgct
    34021 ggctggaaag cgaaccgcaa gattcgcata acgagcatgt cgttattaat gggcagaatt
    34081 tcctgatgga gattacgcct gtttatcttc aggatgaaaa tgatcaacac gtcctgaccg
    34141 gtgcggtggt gatgctgcga tcaacgattc gtatgggccg tcagttgcaa aatgtcgccg
    34201 cccaggatgt cagcgccttc agtcaaattg tcgctgtcag cccgaaaatg aagcatgttg
    34261 tcgaacaggc gcagaaactg gcgatgctaa gcgcgccact gctgattacg ggtgataccg
    34321 gcaccggaaa agatctcttt gcgtatgcct gtcatcaggc aagccccaga gcgagcaaac
    34381 cttacctggc actgaactgt gcgtctatac cggaagatgc ggtcgagagt gaactgtttg
    34441 gtcatgctcc ggaagggaag aaagggttct ttgagcaggc gaacggtggt tcggtgctgt
    34501 tggatgaaat aggtgagatg tcaccacgga tgcaggcgaa attactgcgt ttccttaatg
    34561 atggcacctt ccgtcgggtt ggcgaagacc atgaggtgca tgtcgatgtg cgggtgattt
    34621 gtgctacgca gaagaatctg gtcgaactgg tgcaaaaagg cgtgttccgt gaagatctct
    34681 attatcgtct gaacgtgttg acgctcaatc tgccgccgct acgtgactgt ccgcaggaca
    34741 tcatgccgtt aaccgagctg ttcgtcgccc gctttgccga cgagcagggc gtgccgcgtc
    34801 cgaaactggc cgctgatctg aatactgtac ttacgcgtta tgcgtggccg ggaaatgtgc
    34861 ggcagttaaa gaacgctatc tatcgcgcac tgacacaact ggacggttat gagctgcgtc
    34921 cacaggatat tttgttgccg gattatgacg ccgcaacggt agccgtgggc gaagatgcga
    34981 tggaaggttc gctggacgaa atcaccagcc gttttgaacg ctcggtatta acccagcttt
    35041 atcgcaatta tcccagcacg cgcaaactgg caaaacgtct cggcgtttca cataccgcga
    35101 ttgccaataa gttgcgggaa tatggtctga gtcagaagaa gaacgaagag taagcgtgaa
    35161 tatgcctgat ggtgcaacac catcaggcat attaacttat gctttcagta cagccagagc
    35221 tgcttcgtaa tccggctcgg tggtgatttc atccaccagc tggctgaaaa tcacattgtc
    35281 attttcatca ataaccacaa cggcacgcgc tgccagacct ttcagtgggc catcagcaat
    35341 tgccacaccg taggcttgca ggaattcagc gttacggaaa gtggagaggg tgataacgtt
    35401 gttcagacct tctgcgccgc agaaacgaga ctgggcgaac ggcagatcgg cagagataca
    35461 cagcacaacg gtgttgtcga tctcggttgc cagttggtta aacttacgta ctgatgcggc
    35521 gcaaacaccg gtatcaatac tcgggaaaat gttcagcact ttgcgtttac ccgcaaactg
    35581 accgagggtg acgtcagaca gatcttttgc cacgagagta aaagtctgcg ctttgctacc
    35641 tgcctgcggg atggaattgg cgactgtaac cgggttgccc tggaaatgaa cggtttgtga
    35701 catgattatc tttcctgttt acatatagtt aacgtcacac ctagtttatg ctaactgtca
    35761 ataacacagc aaacgctatt tgcgcttaat ccgcagacca ccgcgacaat aaggagtaaa
    35821 gatgagaacc gttaaggtat ttgaggaggc ctggccctta cataccccgt ttgtgattgc
    35881 tcggggaagt cgcagtgaag cgcgcgtggt ggtggttgaa ctggaagaag aaggtattaa
    35941 aggcaccggt gaatgcacgc cgtatccgcg ttatggagaa agtgatgcct cggtaatggc
    36001 gcaaattatg agcgtcgtgc cgcaattaga gaaagggctg acacgggagg agttgcaaaa
    36061 aatcctccct gccggcgcag cacgtaatgc gctggattgt gcattgtggg atctggcggc
    36121 gcgaaaacag cagcaatcgc tggctgattt gatcggcata acgcttcccg agacagtcac
    36181 cacggcacag acgatagtca tcggtacgcc tgatcagatg gccaatagtg catcaacact
    36241 ctggcaggca ggcgcgaaat tactgaaagt gaagctggat aaccatctta tcagtgagcg
    36301 gatggtggca attcgcacag ctgtgcccga tgcgacgctg atcgttgatg caaatgaatc
    36361 ctggcgtgca gaagggttgg cggcgcgttg ccagctattg gcggatttag gcgttgcgat
    36421 gcttgaacaa ccgcttcctg cgcaggacga tgcggcgctg gagaatttta ttcatccgtt
    36481 accgatttgt gctgatgaaa gttgtcatac tcgtagcaat ctgaaggcgc tgaaagggcg
    36541 ctatgagatg gttaacatta agcttgataa aaccggtggt ctgacggaag cgctggcgct
    36601 ggcgactgaa gcgcgtgcac aaggttttcg tctgatgctg ggctgcatgt tgtgtacctc
    36661 tcgtgccatt agcgccgctt taccgctggt gccgcaggtc agtttcgccg atcttgacgg
    36721 accgacctgg ctggcggtag atgtggaacc ggcgcttcag tttacgacgg gcgaattgca
    36781 tctttaggat gccagcgcag caagtttgcc atcgcaaaga gatatttttc gctggcttcg
    36841 tcggaggaga tgggtggaaa ctctgcggtg atgcaatgca agttgagatc tgcacaccaa
    36901 ctgccgaaag agccaggcgt ttcataaccg acgctggtta ccagaggcaa ttcaaacgcc
    36961 tgggccagcc attcacctaa ttcgctgtgt ctgggatctt caatacaggc cagcggatcg
    37021 tggaatgaga ccacccaggc aggttgaatg cggtgtataa gctggcacag cgcctgggtt
    37081 tcaggttcgg agccaggttt gtcacctgtc agtaaaacga catcacgttc ttcagcggcg
    37141 ctgttccagc gataaactgt ttcaccttcc ttccagttcg ctgccggaaa gtttcggttt
    37201 aaatccacac cattagcatt ggcccgtaac cctaactggc agccgtcagg attcacacac
    37261 agcaccacat gatggcggcg caaagaaggt gtcaaagtcc gcagagcaca ggagagcgtg
    37321 acgatcgaag agttttcatc accgtgagtg ccagccagaa tcaaaccact ttcgtgactg
    37381 gctgcagggg ccggaaacca gattaacggc gcgcccaata atgaacgtcc gtaatgttct
    37441 gttccgggcg gaaatgcgcc gcgttcggcg cgtgggcgag ttacggtcat gatcgtctct
    37501 ggttcaggaa gtattacagg cagtgttgtg caaattttgc ggattatcaa atccttttct
    37561 tcaacgctat gtcatcgatc ttgtatcact tttttgccgg aagttgtttg tattttcatt
    37621 gatgtgcaga tattagaatt gtcgtaatgt tatatacatg gcgtatttta ttcttactgg
    37681 ttctacacat gttaatcgca tctgattgat cagttcacct taataagttt aataaatcgt
    37741 actgcacata gtacagtacg atggtagcta tataattgat ttacatgaag aagggcttat
    37801 caccatcagt cccgggttga ttgatgccta tattctcacc aatgtgtttc tcaggtttat
    37861 cgatgatatc tgtgattaat gctgcgacac tcttacgtga tacgatcgta cctttgaatg
    37921 gttcattgcg tgaggtaagc tcataatcaa taatatcttc atcagtcagc caggcagggc
    37981 gaagaattgt atattccagt ccagaagcct caattgcatc cgcagccctg cggaatggtt
    38041 ttaggggttc accaataact gcattattcc attcgccaaa ttttcctgga acttcatcat
    38101 aaataccgag ggaaagaaca aagatcagac gtttaacatc acaggctttc atcgcagcga
    38161 tcactgaatt tgcctgaata tctaaatctt cccccgtaag gttggcatac acaacatcct
    38221 gtccctgcat ggcctgcttc agtgctgcat gattcagtac atcccccata ataatcttgc
    38281 tattagttgg atagggtttg tggatttttg ctggctgacg ggcaaataaa gtttgtttaa
    38341 tggtttgttt atctgccagt tgattaatca catggcgggc tatctgaccg ccagcaccaa
    38401 gaatcagtat atttttcatg aaacttttcc cagattattg gcagcaatat gccaggccat
    38461 atcactatta cggatttgaa tatgtttcat tacagagttt cctcaaagaa cggagctaat
    38521 actgaaattg cttcagccac atagactttg ccgtcataaa gatccatatg gtttgcgcct
    38581 tcaacaatgt gatagcgttt atccttgctt gatgctcgat cgtacaggtc gtcactcatc
    38641 catttgctcc cagcctggct gcccgccaca atctgtatcg gctgagtcag gtacacttcc
    38701 gccatatggt aagcatcata ggtaataatc tggttaaggc tgcgcaaagt agcgtaaccc
    38761 ggtgctgttg gatactgcgc gcgaggggtg tggtaatact cccaggcctg acgcagttct
    38821 tcgttcggcg catcggactc cttcattggt gccagtggca tagtggcgta ttctccgctg
    38881 ctaatatcgc tggttcgggc gtctgaaccc gcgtcaacgt atggtagggc atcaatagat
    38941 ttcacattgt tttcccaacc attacggaac atcgaaccaa tattgaccgc actaacagta
    39001 ccgatggcct tgatgcggcg atcctgaatt gcagcattgg ctgtatatcc tgctccggca
    39061 caaattccca tcgcaccaat tcgggtattg tcgacataag aaagcgttgt caggtaatca
    39121 atcacggcac tgacgtcttc agtacgaatg tatgggtttt ctaactgacg cggctcgccg
    39181 ccgctttcac cttgataaga tgcgtcataa gcaatagtga caaatccctt ttccgccagt
    39241 ttttcggcat aggttccggc cgtttgctct ttaacgcccc cacctgggtg agataccaca
    39301 attgccggat actgacgggt ttcatcaaat tttggaggga aatagatcac tgcagacaaa
    39361 gagatggttg gattattgct gttagtgaag ctgactttat tattcatcat tccgttcctc
    39421 atggagttgt cggttcgttt taacggttgg tgatatcact atagatattg atcattaagt
    39481 tgattagacc caaaatcatg attagaccta tcatttaaat gattaataga ttgtatggag
    39541 atactgaatg aagcgtgaag aaatcgctga tctgatggcg tttgtcgttg ttgcagagga
    39601 gcgtagcttc actcgcgcag cagcccgcct gagcatggcg cagtcagctt taagccagat
    39661 agtgcgccgt atagaagaac gattgggatt gcggcttctg acgcgaacca cgcgcagcgt
    39721 tgtacccact gaagcgggcg agcatctttt gtctgttctt ggccctatgt tgcatgacat
    39781 agattctgcc ctgacatccc tgagcgatct gcagaaccgc ccatccggga caatacgtat
    39841 tactactgta gaacatgcag caaaaacgat attgttacca gctatgcgca cattcctgaa
    39901 atcgcatcct gaaattgata ttcagcttac cattgattat ggtttgaccg atgtcgtttc
    39961 tgaacgtttt gatgcaggcg tccgtctggg tggggagatg gataaagata tgagcgccat
    40021 tcgaatcggg ccagacatac caatggctat tgttggctca ccggattatt tttctcgtcg
    40081 aagtgctcca acgtcagtgt cacaattaat agatcatcag gcaattaatt tgtatcttcc
    40141 cacatcgggt acagcaaatc gctggagatt aatacgcggt ggacgtgaag ttcgtgttcg
    40201 catggaaggt cagcttttac tgaatacgat agacctgatc attgatgctg caattgatgg
    40261 gcatggattg gcgtatctac cttatgatca ggttgagcgg gctattaaag aaaaaaaact
    40321 gatacgtgtt ttggataaat tcacaccaga tttacccggt tatcacctgt actatccaca
    40381 ccgtcgacat gctggctcgg cattctcatt atttatagat aggctgaagt ataaaggtgc
    40441 tgtttagcac tacttgctga tacattaatt taattcttct cttaacgtat tctcagttcc
    40501 tttcaacgtt ttggttattt tttattcttc gcacaatggc gacagatgct gattatgata
    40561 ccgaaaacgg gtttgaacgt gcgaagcccg aacgtagtgt tcggagttct atgtgcttta
    40621 ccgcattttg gagactatta tttacactaa atctgatttg atatattgat acttaaaaca
    40681 tttgatgctt cctttgtcac ttttttgatg gaagttgttt gcatttcttt aaggcgaaac
    40741 aaataattac gcatcaattt taatgtcggt tagagggaaa cttatgaagc actctgtttc
    40801 agtcacgtgt tgtgcgctgt tggtcagcag catttctctt tcgtatgctg ctgaagttcc
    40861 gagcggcaca gtactggcag agaagcagga gctggtgcgc cacattaaag atgagccagc
    40921 gtcgctggat cccgctaaag ccgtgggcct gccggagatt caggtcattc gcgatctgtt
    40981 tgaaggactg gtgaatcaga acgagaaagg ggagattgtc cccggcgttg cgactcagtg
    41041 gaaaagtaat gacaaccgta tctggacttt taccctgcgc gataacgcaa aatgggcgga
    41101 tggcacaccg gtaacggcgc aagattttgt ctacagctgg caacgtctgg tggacccgaa
    41161 aacgctatcg ccatttgcat ggtttgccgc gctggcggga atcaacaacg cacaggcgat
    41221 tattgatggt aaagccgcgc ctgaccagct tggcgtcacc gcagttgatg cccatacttt
    41281 gaaaatccag cttgataaac cattgccgtg gtttgtgaat ttaaccgcta actttgcctt
    41341 cttcccggtg caaaaagcca acgtagaaag cagtaaagag tggacgaaac ccggaaatct
    41401 gatcggcaat ggcgcttatg ttcttaaaga gcgcgtagtc aatgaaaaac tggtcgtggt
    41461 gccgaatacc cattattggg ataacgccaa aacggtactg caaaaagtga ccttcctgcc
    41521 aattaatcag gaatccgcag ccactaagcg ttttctcgcg ggggatattg atatcaccga
    41581 atccttcccg aaaaatatgt atcagaagct gttgaaggat attccggggc aggtttatac
    41641 gccgccgcag ctcgggacct attattatgc gtttaacacg caaaaagggc cgacggcaga
    41701 tcagcgcgtt cgcctggcat taagtatgac gatagatcgc cgcctgatga ccgaaaaagt
    41761 gttggggacg ggcgaaaagc cagcgtggca ttttacacca gatgttaccg cgggatttac
    41821 gccggaacct tcgccgtttg aacaaatgag tcaggaagaa ctgaatgcgc aggcaaaaac
    41881 tttgttgagc gccgctggtt atggtccgca aaaaccgctg aagctgacgc ttttgtataa
    41941 cacttcagaa aaccatcaaa aaattgcgat tgctgtagca tcgatgtgga aaaagaacct
    42001 gggcgtagat gttaaattgc aaaaccagga atggaaaacc tatatcgata gccgtaatac
    42061 cggcaatttt gatgttatcc gcgcctcgtg ggtaggggat tataatgaac cctccacttt
    42121 cctgacatta ttaacgtcaa cgcattccgg gaatatttca cgctttaaca atccggcata
    42181 tgacaaagtt ctggcccagg catcgacgga aaataccgtt aaagcgcgta atgccgatta
    42241 caacgcggca gaaaaaatcc tcatggagca agcaccgatt gcaccaattt atcaatatac
    42301 caatggacga ttaatcaagc cgtggctgaa aggttatccc attaataacc ctgaagatgt
    42361 ggcgtacagt cggactatgt atattgtgaa gcattaatgt gatgggaact ggcgttaccc
    42421 ttgtggataa cgccagtgat gtctgattta gcgaccctgg tccggcggtg taatgttatc
    42481 catatacagc gtctggctgg ggaaggcaaa gtccgcgccg tgtgactgta caatatcgat
    42541 aatcttcaaa taaacgtctt gctgtgcagc aagccactca gcccataccg tggttttggt
    42601 aaagcaataa accataatat ttaatgacga atcagcaaac tggttgaaat aaaccagtaa
    42661 ggtttgtcgc tggtcgatgg ccgggtgatt tttcagcatc tcacgtacgg cttcgacaat
    42721 aacgcccact tttgccgcat cctcataacg taaaccaatg gtcgtggtaa tgcgccggtt
    42781 ggtcattcgt ccaggatttt ctacgctgat cgacgaaaac agcgagttcg gtacgtacaa
    42841 tggacgatta tcaaaggtct taattttggt aattcgccag ccaatttccg ctactgtacc
    42901 ttcgatattt ctgtccggtg aacggatcca gtcgccaata ctgaaaggac ggtcgaaata
    42961 gagcataatc ccggaaaaga agttactcag aatatcttta ccggccatac cgacagccag
    43021 accaccaata ccaccaaagg tcaacaagcc agaaaggctc atgccgaaat gttcgccata
    43081 aagcagaaca agcaccacaa taatggtgat tttgataatg cgcgacatga tccgcgcact
    43141 ggtgatatcg cgaccttttt taatctgctg tttttcaaac tgattaatca gcagaaatag
    43201 cttaatcgtc agaataaccg caatcaggga cgtacagata aaatcgataa cgcctggggt
    43261 gataaatttg
//
LOCUS       DADTFG010000019        42477 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.019, whole genome shotgun sequence.
ACCESSION   DADTFG010000019 DADTFG010000000
VERSION     DADTFG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.019"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            33..596
                     /gene="srlA"
                     /locus_tag="J1138_001017"
                     /gene_synonym="gutA"
     CDS             33..596
                     /gene="srlA"
                     /locus_tag="J1138_001017"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBA8452851.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            593..1552
                     /gene="srlE"
                     /locus_tag="J1138_001018"
                     /gene_synonym="gutE"
     CDS             593..1552
                     /gene="srlE"
                     /locus_tag="J1138_001018"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBA8452852.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1563..1934
                     /gene="srlB"
                     /locus_tag="J1138_001019"
                     /gene_synonym="gutB"
     CDS             1563..1934
                     /gene="srlB"
                     /locus_tag="J1138_001019"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBA8452853.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1938..2717
                     /gene="srlD"
                     /locus_tag="J1138_001020"
     CDS             1938..2717
                     /gene="srlD"
                     /locus_tag="J1138_001020"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBA8452854.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2823..3182
                     /gene="gutM"
                     /locus_tag="J1138_001021"
     CDS             2823..3182
                     /gene="gutM"
                     /locus_tag="J1138_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBA8452855.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHVGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3249..4022
                     /gene="srlR"
                     /locus_tag="J1138_001022"
                     /gene_synonym="gutR"
     CDS             3249..4022
                     /gene="srlR"
                     /locus_tag="J1138_001022"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBA8452856.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4015..4980
                     /gene="gutQ"
                     /locus_tag="J1138_001023"
     CDS             4015..4980
                     /gene="gutQ"
                     /locus_tag="J1138_001023"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBA8452857.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4977..6491)
                     /gene="norR"
                     /locus_tag="J1138_001024"
     CDS             complement(4977..6491)
                     /gene="norR"
                     /locus_tag="J1138_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBA8452858.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGEAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6678..8117
                     /gene="norV"
                     /locus_tag="J1138_001025"
     CDS             6678..8117
                     /gene="norV"
                     /locus_tag="J1138_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBA8452859.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIIINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8114..9247
                     /gene="norW"
                     /locus_tag="J1138_001026"
     CDS             8114..9247
                     /gene="norW"
                     /locus_tag="J1138_001026"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBA8452860.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIIGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLGAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WHIYTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(9471..11723)
                     /gene="hypF"
                     /locus_tag="J1138_001027"
     CDS             complement(9471..11723)
                     /gene="hypF"
                     /locus_tag="J1138_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBA8452861.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLHGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LEASCHGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11876..12403)
                     /gene="hydN"
                     /locus_tag="J1138_001028"
     CDS             complement(11876..12403)
                     /gene="hydN"
                     /locus_tag="J1138_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBA8452862.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12552..13562)
                     /locus_tag="J1138_001029"
     CDS             complement(12552..13562)
                     /locus_tag="J1138_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA8452863.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELITAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIAFNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SR"
     gene            13822..15279
                     /gene="ascF"
                     /locus_tag="J1138_001030"
     CDS             13822..15279
                     /gene="ascF"
                     /locus_tag="J1138_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBA8452864.1"
                     /translation="MAKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDLQPAPPVGKPKLTLRRI
                     GAGVLDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLIILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAALGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNSELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            15288..16712
                     /locus_tag="J1138_001031"
     CDS             15288..16712
                     /locus_tag="J1138_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA8452865.1"
                     /translation="MSVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPSDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSYWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16836..17306)
                     /gene="hycI"
                     /locus_tag="J1138_001032"
     CDS             complement(16836..17306)
                     /gene="hycI"
                     /locus_tag="J1138_001032"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBA8452866.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE"
     gene            complement(17299..17709)
                     /locus_tag="J1138_001033"
     CDS             complement(17299..17709)
                     /locus_tag="J1138_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HBA8452867.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(17706..18473)
                     /gene="hycG"
                     /locus_tag="J1138_001034"
     CDS             complement(17706..18473)
                     /gene="hycG"
                     /locus_tag="J1138_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBA8452868.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(18473..19015)
                     /gene="hycF"
                     /locus_tag="J1138_001035"
     CDS             complement(18473..19015)
                     /gene="hycF"
                     /locus_tag="J1138_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBA8452869.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFDLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(19025..20734)
                     /locus_tag="J1138_001036"
     CDS             complement(19025..20734)
                     /locus_tag="J1138_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HBA8452870.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(20752..21675)
                     /gene="hycD"
                     /locus_tag="J1138_001037"
     CDS             complement(20752..21675)
                     /gene="hycD"
                     /locus_tag="J1138_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBA8452871.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPPLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(21678..23504)
                     /gene="hycC"
                     /locus_tag="J1138_001038"
     CDS             complement(21678..23504)
                     /gene="hycC"
                     /locus_tag="J1138_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBA8452872.1"
                     /translation="MSAISLINSGVAWFVAVAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGTVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(23501..24112)
                     /locus_tag="J1138_001039"
     CDS             complement(23501..24112)
                     /locus_tag="J1138_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA8452873.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24237..24698)
                     /gene="hycA"
                     /locus_tag="J1138_001040"
     CDS             complement(24237..24698)
                     /gene="hycA"
                     /locus_tag="J1138_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBA8452874.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVTGQSPAA"
     gene            24910..25260
                     /gene="hypA"
                     /locus_tag="J1138_001041"
     CDS             24910..25260
                     /gene="hypA"
                     /locus_tag="J1138_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBA8452875.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            25264..26136
                     /gene="hypB"
                     /locus_tag="J1138_001042"
     CDS             25264..26136
                     /gene="hypB"
                     /locus_tag="J1138_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBA8452876.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRAHFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26127..26399
                     /gene="hypC"
                     /locus_tag="J1138_001043"
     CDS             26127..26399
                     /gene="hypC"
                     /locus_tag="J1138_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBA8452877.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            26399..27520
                     /gene="hypD"
                     /locus_tag="J1138_001044"
     CDS             26399..27520
                     /gene="hypD"
                     /locus_tag="J1138_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBA8452878.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            27517..28527
                     /gene="hypE"
                     /locus_tag="J1138_001045"
     CDS             27517..28527
                     /gene="hypE"
                     /locus_tag="J1138_001045"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBA8452879.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            28601..30679
                     /gene="flhA"
                     /locus_tag="J1138_001046"
     CDS             28601..30679
                     /gene="flhA"
                     /locus_tag="J1138_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBA8452880.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            30724..31152
                     /locus_tag="J1138_001047"
     CDS             30724..31152
                     /locus_tag="J1138_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBA8452881.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(31191..31535)
                     /locus_tag="J1138_001048"
     CDS             complement(31191..31535)
                     /locus_tag="J1138_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBA8452882.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWVGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            31821..34382
                     /gene="mutS"
                     /locus_tag="J1138_001049"
     CDS             31821..34382
                     /gene="mutS"
                     /locus_tag="J1138_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBA8452883.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVSQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            34488..35144
                     /gene="pphB"
                     /locus_tag="J1138_001050"
     CDS             34488..35144
                     /gene="pphB"
                     /locus_tag="J1138_001050"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBA8452884.1"
                     /translation="MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDHGPESLNVLRLLNQPWFTSAKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(35195..35992)
                     /locus_tag="J1138_001051"
     CDS             complement(35195..35992)
                     /locus_tag="J1138_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8452885.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GRCIYLDAGTTTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            36158..37066
                     /locus_tag="J1138_001052"
     CDS             36158..37066
                     /locus_tag="J1138_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA8452886.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGSEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            37063..38325
                     /locus_tag="J1138_001053"
     CDS             37063..38325
                     /locus_tag="J1138_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBA8452887.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVES
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            38322..38960
                     /locus_tag="J1138_001054"
     CDS             38322..38960
                     /locus_tag="J1138_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBA8452888.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            38965..39741
                     /locus_tag="J1138_001055"
     CDS             38965..39741
                     /locus_tag="J1138_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBA8452889.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMSGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            39830..41194
                     /locus_tag="J1138_001056"
     CDS             39830..41194
                     /locus_tag="J1138_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBA8452890.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(41337..42380)
                     /locus_tag="J1138_001057"
     CDS             complement(41337..42380)
                     /locus_tag="J1138_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBA8452891.1"
                     /translation="MQGIKIYTILASSLISGSVFAQTEISTANSSTVNASYVEPSAEK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
BASE COUNT        10108 a        11404 c        11193 g         9772 t
ORIGIN      
        1 taataaaaca ataaatactg aaggagagaa caatgataga aaccattact catggcgcag
       61 agtggttcat cgggctgttc cagaaaggcg gagaagtgtt taccgggatg gtaaccggca
      121 ttcttccgct gttaattagc ctgctggtta tcatgaacgc attgattaat tttatcggtc
      181 agcatcgtat tgaacgattt gctcaacgtt gcgccggtaa ccctgtttcc cgttacctgc
      241 tgctaccgtg cattggtacg tttgtctttt gtaacccgat gaccttaagt cttggtcgct
      301 ttatgccgga aaaatacaaa cccagctact acgcagcggc ctcttatagc tgccactcaa
      361 tgaatggcct cttcccccat atcaaccctg gcgaactgtt tgtttatctt ggcattgcca
      421 gcggtctgac gacgctgaat ctgccacttg gcccactggc agtgagttat ctgctggttg
      481 gtttggtcac caacttcttc cgcggctggg taaccgatct gaccaccgct attttcgaga
      541 aaaagatggg cattcaactt gaacaaaaag ttcacctggc aggagcaaca tcatgacacg
      601 cattcggatc gaaaaaggaa cgggtggctg gggcggcccg cttgagctgg aagccacgcc
      661 gggcaaaaaa atcgtctata tcaccgccgg tacccggcct gcgattgttg acaaactggc
      721 acagcttact ggctggcaag ctatcgacgg atttaaagaa ggtgaacccg cggaggcgga
      781 aattggtgtc gcggtaatcg actgtggcgg cacattacgc tgcggcatct atccgaaacg
      841 gcgtattccc accattaata tccactcgac gggcaagtcc ggcccgctgg cgcagtacat
      901 tgtggaagat atttatgtct ctggtgtaaa agaagaaaac atcactgtag tgggtgaggc
      961 gacaccacaa ccctcttccg tgggccgtga ctatgacacc agcaagaaaa tcaccgaaca
     1021 aagcgatggt ttactggcga aggtgggaat gggtatgggt tctgccgttg ccgtgttgtt
     1081 tcaatctggt cgtgacacca tcgacactgt attaaaaacc attctgccgt ttatggcgtt
     1141 cgtctcggcg cttatcggca tcattatggc ttccggtctt ggcgactgga ttgcccacgg
     1201 tcttgctccg ctggcgagcc atcctctggg gctggtcatg ctggcgctca tctgctcctt
     1261 cccgctgctt tcacctttcc tcggcccagg cgcagttatc gcacaggtta tcggcgtatt
     1321 gattggcgtg cagattggtc tcggcaatat tccgccgcat ctggctttac ctgcactgtt
     1381 tgccatcaac gcgcaggcgg cctgcgactt catcccggtc ggtttgtcgc tggcggaagc
     1441 tcgtcaggac acggttcgcg tcggtgtccc ttctgttctg gtgagccgct ttttaaccgg
     1501 cgcgccaact gtactgatcg cctggtttgt ctccggtttt atctatcaat agaggctgaa
     1561 acatgaccgt tatttatcag accaccatca cccgtatcgg cgcgagtgcc actgacgccc
     1621 tcagcgacca gatgctcatc acctttcgtg aaggcgcgcc tgcggatctc gaagagtatt
     1681 gcttcattca ttgccacggc gagttgaaag gtgcactcca tcccggtttg caattttctc
     1741 tcgggcggca tcgctatccg gtgaccgccg ttggcagcgt ggcggaagac aaccttcgcg
     1801 aactgggtca tgtcaccctg cgcttcgatg gtttaagcga agcggaattt ccgggcactg
     1861 tccatgtggc aggccctgta cccgacgata tcgcgccggg atcggttttg aagtttgaat
     1921 ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat cggtggtggg caaaccttag
     1981 gcgcgttcct gtgccacggt ctggctgccg aggggtatcg cgtggcggtt gtcgatattc
     2041 agagcgacaa agccgcaaat gtggcacaag aaattaacgc cgaatatggt gaaggaacag
     2101 cgtacggttt tggtgctgac gccaccagcg agcaaagcgt tctggcgctt tctcgtgggg
     2161 tagatgaaat ctttggtcgc gtggatttgc tggtctacag cgccggaata gcaaaagcag
     2221 cctttatcag cgacttccag ctcggcgatt ttgaccgttc gctacaggtg aatctggtgg
     2281 gttatttcct gtgtgcccgt gaattttcgc gtttgatgat ccgcgacggg attcagggtc
     2341 gcattattca gatcaactcg aaatccggca aagtgggcag caaacacaac tctggctaca
     2401 gcgcagcgaa atttggtggc gtcgggctga ctcaatcact ggcgctggat ctggcggagt
     2461 acggcattac ggtgcattcg ctgatgctcg gtaacctgct gaaatcgcct atgttccagt
     2521 cactgttgcc acaatacgcg accaagctgg gtatcaaacc ggagcaagtc gagcagtatt
     2581 acatcgataa agtaccgctc aaacgcggct gcgattatca ggatgtgctg aatatgctgc
     2641 tgttctacgc cagtcctaag gcgtcgtact gcactggtca gtcgatcaat gtcaccggcg
     2701 gtcaggtgat gttctgatca acagcggaga tcttttaagg atctccgcgc gactaataga
     2761 atgcctgatg cgctacgctt atcaggccta caggacttcc gccactacat tacggagaag
     2821 ttatggtatc cgcactcatc accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag
     2881 gcggttggca aatttctcgt tttaaccgtg ccttcgacac gctatgccag caagggaggg
     2941 ttggcgtggg ccgttccagc gggcgcttta aaccgcgggt cgtggtcgct attgcgctgg
     3001 acgatcagca gcgcatcgtc gacaccttgt ttatgaaagg actgaccgtc ttcgcccgac
     3061 cgcaaaaaat tcccgcaatt accggcatgc atgtgggtga tttacagccc gatgtgatct
     3121 ttccccatga tccactatca cagaatgctc tatcattggc gcttaaactg aaacgtggat
     3181 aatttcgttg tgaatgttac ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa
     3241 tcacgcccat gaaacctcgt cagcgtcagg ccgccattct ggagtatcta caaaagcagg
     3301 gtaaatgctc ggttgaagaa ttggcgcaat actttgacac cacaggcaca accattcgca
     3361 aagatctggt cattctggaa catgccggaa ccgtcattcg tacttatggc ggcgtggtgt
     3421 tgaacaaaga ggaatccgat ccgcctatcg atcataaaac gcttatcaac acccacaaaa
     3481 aagagctgat tgcagaagct gccgttggtt ttattcatga tggcgactca ataattcttg
     3541 atgctggcag taccgtgttg cagatggtgc ctctgctatc gcgctttaat aacatcacgg
     3601 tgatgaccaa cagcctgcac atcgtcaatg cgctatccga actggataac gaacaaacta
     3661 tcctgatgcc aggcggaaca tttcgcaaaa aatcggcctc atttcacggg caactggcag
     3721 agaatgcctt cgagcatttc accttcgata aattgtttat gggcaccgac ggcatcgatc
     3781 tcaatgcggg cgtaaccacc tttaacgagg tctataccgt cagtaaggcg atgtgtaatg
     3841 ccgcgcgcga agtgattttg atggctgact catcgaagtt tggtcgtaaa agccccaacg
     3901 tggtttgtag tcttgaaagc gtcgataagc tgattaccga cgcaggtatc gatccagcgt
     3961 ttcgtcaggc gctggaagaa aaagggatcg acgtgatcat aaccggagag agcaatgagt
     4021 gaagcactac tgaacgcggg acgtcagacg ttaatgctgg agttgcagga agcaagccgt
     4081 ttaccagaac gtctgggcga tgattttgtt cgcgccgcca atatcatcct gcactgcgaa
     4141 ggcaaagtgg tggtttcggg aattggcaaa tcgggccaca ttggtaagaa aatcgcggca
     4201 acgcttgcca gcaccggcac accggctttt tttgtccatc cggcagaagc actgcacggc
     4261 gatctgggga tgattgaaag ccgcgatgtg atgctgttta tctcttattc cggtggcgcg
     4321 aaggaactgg atctcattat tccgcgtctg gaagacaaat ctatcgcgct gctggcaatg
     4381 accggaaaac cgacgtcacc gctgggcctg gcggcaaaag cggtgctgga tatctccgta
     4441 gaacgcgaag cctgcccgat gcaccttgcg ccgacttcca gcaccgtgaa taccctgatg
     4501 atgggcgacg cgctggcgat ggcggtcatg caggcgcgtg gttttaatga agaagatttt
     4561 gcccgctccc acccagccgg ggcactgggc gctcgcttgc tgaacaaagt gcatcatctt
     4621 atgcgtcgtg acgatgccat cccgcaggtg gcgttaaccg ccagcgtgat ggacgcgatg
     4681 ctggaactta gccgcaccgg tttaggtctg gtggcagtgt gcgatgatca acgactggtg
     4741 aaaggcgtct ttaccgacgg tgatttacgt cgctggctgg ttggcggcgg cgcactcacc
     4801 acgccagtca atgaagcgat gacggtcggc ggcaccacgt tgcaatcgca aagccgtgcc
     4861 atcgacgcca aagaaatcct gatgaaacgc aaaatcaccg ccgcaccggt agtggatgaa
     4921 aacggcaaac tcaccggcgc aattaacctg caggatttct atcaggccgg gattatttaa
     4981 tccttcagcc ccagacgttt cgccagccga tgcaggttgg cgacgtcggt ttccagcatc
     5041 cgcgcacagg cggcccagtt gtgatgattt tgcgccagtg cctgacgaat ggtttcacgc
     5101 tggaacgctt ctgtcgcttc acgcagattt tgcttaacaa cgggcaccgc cgccgcttct
     5161 ggcggcggca acgtcacctc aggaaaagca aaatgttgcg cctcaagaat cacttcatcg
     5221 ccgctgcggg tggctctcga caatactacc gcccgatgaa tagcatgttc cagttcgcgc
     5281 acgtttcccg gaaaattgta gtgttgcagt aaatttcgcg ctccggcact taataccacg
     5341 cgggagagcc ccagccgcaa acgacactgc tcgcagaaat accccgccag cagaatgaca
     5401 tcatcgcccc gctcacgcag cggcggcact gaaagtggaa acacgctcag gcggtggaac
     5461 aagtcagcgc gaaatcgccc tgccagtacc tcttcgcgta agtcacggtt agtcgccgcc
     5521 agcacgcgca catcgacccg caaactgcgg tcatcgccaa cgcgttgaat atcgccatac
     5581 tgcaacaccc tcagcagctt ggcctgcaat gccaacgaca actcgccgat ctcatcgaga
     5641 aacagcgtgc cgttatccgc catttcaaac ttcccgctgc ggttactgat agcaccagta
     5701 aacgcccctt tcacatgccc gaacaactca ctttccgcca cactttccgg cagtgcggca
     5761 cagttgagat agaccagcgg attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc
     5821 accagctcct taccggttcc cgtctcaccg ctaattaaaa cgttgagatc ggatgccgcc
     5881 acaatctcaa tctctttttt caattgcgtc atgcctggcg acagaccgat catctgcgtc
     5941 tgtttcaccg cttcaaacgg cgcggcttcg cctggcagca tattctggct ttccagttgt
     6001 tcaatcagta acgcattgct taacgctccc gccgccagcg cagcaatcag gcgtaactct
     6061 tcgtcgctga aaacatcgaa ctgatcgggc tgcatcccgt cgagcgtcaa ggcaccgatc
     6121 aggttttgcc cggcaaacaa cggcaaacca acgcaggcgt gaaccttcag actctcttgc
     6181 cctggaatca gaccgtcata aggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca
     6241 tcacccgcgc gggcaatcgc ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg
     6301 agtacatcct tcgccagccc gtcgatggca agcggaataa actgccgcga gtcgtaacgt
     6361 agcaacgcag acgcatcgca ctccagcacc tgacgcagcg tggtgatcag gcgctgaaaa
     6421 cgatcctgat gaccaatccc acgctgcaat tcgatggcga tattcgccag cacatcaacg
     6481 gaaaaactca tctttgcctc actgtcaatt tgactataga tattgtcata tcgaccattt
     6541 tattgatagt cattttgact actcattaat gagcacaatt ttatttatag agtaaaaaca
     6601 attagataaa aaactggcac gcaatctgca atcagcaaga catcttttta gaacacgctg
     6661 aataaattga ggttgctatg tctattgtgg tgaaaaataa cattcattgg gttggtcaac
     6721 gtgactggga agtgcgtgat tttcacggta cagaatataa aacgctgcgc ggcagcagct
     6781 acaacagcta cctcatccgc gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat
     6841 tcagccgcga atttgtgcag aacctgcgta atgaaatcga tctggcggat atcgattaca
     6901 tcataattaa ccatgctgaa gaggaccacg ccggggcgct gaccgaactg atggcacaaa
     6961 ttcccgatac gccgatctac tgtactgcca acgctatcga ctcgataaat ggtcatcacc
     7021 atcatccgga gtggaatttt aatgtagtga aaactggcga cacgctggat atcggcaacg
     7081 gcaaacaact catttttgtc gaaacgccaa tgctgcactg gccggacagc atgatgactt
     7141 acctgacagg cgacgcggtg ctgttcagta acgatgcttt cggtcagcac tactgcgacg
     7201 agcatctgtt caacgatgaa gtggatcaga ctgagctttt cgagcagtgc cagcgttact
     7261 acgccaatat cctgacgccg ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg
     7321 gctttaactt gccagtcgat atgatagcga cctctcacgg cgtggtatgg cgcgataacc
     7381 cgacgcaaat tgtcgagctg tacctgaaat gggcggcgga ttatcaggaa gacagaatca
     7441 ccattttcta cgacaccatg tcgaataata cccgcatgat ggccgatgct atcgcccagg
     7501 gtattgcgga aaccgaccca cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata
     7561 aaaacgaaat cctgaccaat gtcttccgct caaaaggcgt gctggtcggc acttctacga
     7621 tgaataatgt gatgatgccg aaaatcgccg ggctggtgga ggagatgacc ggattacgct
     7681 tccgtaacaa acgcgccagc gctttcggct ctcacggctg gagcggcggt gcggtggatc
     7741 gtctttccac gcgcctgcag gatgcgggtt tcgaaatgtc gcttagcctg aaagccaaat
     7801 ggcgaccaga ccaggacgct ctggagttat gtcgtgaaca cggtcgcgaa atcgcccgtc
     7861 agtgggcgct cgcgccgctg ccgcagagca cggtgaatac ggtggttaaa gaagaaacct
     7921 ctgccgccac gacggctgac ctcggcccac ggatgcaatg cagcgtctgc cagtggattt
     7981 acgatccggc aaaaggcgag ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag
     8041 tcccggataa cttcctgtgc ccggaatgct ctctcggcaa agacgtcttt gacgaactgg
     8101 catcggaggc aaaatgagca acggcattgt gatcattggt tcgggcttcg ccgcccgcca
     8161 actggtgaaa aatattcgca aacaggacgc cagtattcca ttaaccctga ttgccgccga
     8221 cagcatggat gagtacaaca aacctgacct cagccatgtt atcagtcagg ggcaacgtgc
     8281 cgacgacctt acccgccaga cggcaggtga atttgccgag cagtttaatc tgcgcctgtt
     8341 tccgcacacc tgggtaacgg atatcgatgc cgaagcccat gtggtgaaaa gtcagaataa
     8401 tcagtggcaa tacgacaagt tagtgctggc aaccggtgcc agcgcctttg tcccgccagt
     8461 gcccgggcgt gagttaatgc tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac
     8521 gcaactgcgg gatgcccgac gcgtgttgat tatcggcggt ggtttgattg gtagcgaact
     8581 ggcgatggat ttttgtcggg caggcaaaat ggtcacgcta atcgacaacg ctgccagtat
     8641 tctggcgtcg ttaatgccac cggaagtcag cagccgcttg cagcatcggt tgacggagat
     8701 gggcgttcat ctgctgttga aatctcagtt gcagggacta gaaaaaacag attctggcat
     8761 tctggcaacg ctggaccgcc agcgttgcat cgaagtggat gcggtaattg ccgccaccgg
     8821 actgcgcccg gaaaccgccc tggcacgacg cgccgggctg acgattaatc gcggcgtttg
     8881 cgtcgatagt tatctgcaaa ccagtaatgc cgatatttat gcgctgggcg attgcgcgga
     8941 aattaacggt caggtattgc cgttcctcca gccgattcaa cttggcgcaa tggtgctagc
     9001 aaaaaatctt ctcggcaata acacgccgct gaaactcccg gcgatgctgg tgaaaatcaa
     9061 aacgccggaa ttaccgctgc atctggcagg cgaaacccag cgtcaggatt tacgctggca
     9121 catttatacc gaacgccagg gaatggtggc gcgcggcgtt gacgatgctg accagcttcg
     9181 cgcctttgtt gtcagtgagg atcggatgaa agaggcattt ggattgttga aaacgttgtc
     9241 gatgtaggcg agctacagtg ccccaaatgt cggatgcggc gctgacgcgt cttatccgac
     9301 ctacagggaa cgcatgtgtt gatcacataa tacgtttatg tcgcatcctg cactgtccca
     9361 aaatgtcgga tgcgacgctg gcgcgtctta tccgacctac ggggacgcac gtgtaggccg
     9421 gataaggcgt ttacgccgca tccggcaatg gtgtccaaat gcaacacgct ttatccgttc
     9481 tggacttcac ccgctaacca gcgcgccgca gcaataaccc cctgccccag agacaatcca
     9541 ccatcgcccg ccggtaaact ctgcgggaag agcaacgtaa aatcagcaag ataatgcgcc
     9601 agacgtgcac gcagcaaacg gttatgaata actccgccgc taaataccag cgtagtgata
     9661 ccccgcatcg tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga
     9721 aacgcccacg cgcgttgatt aaccggagcc tgccagctca gccactgctg ccagaaagtg
     9781 gcgagatcca gttgattgtc caccagcggc atcgtcaccg gatgcgtcac gccgtggcac
     9841 gaagcctcca gcgcctccag cgcacaagcc gcttcacctt cataacttaa cgtggctggc
     9901 gcacagccca gcgccgccgc tacggcatcg aacaaacgtc cacacgacga tgccagcggc
     9961 gcgttaattc cacgctcaat agcccgcgcc agcacgctcc agttttgctg ttgcacactt
    10021 gccgtttcag agtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt
    10081 cgccacggct gcttcgcagc caaatcgccc cccggaagcg ccactgcggg caagccgccc
    10141 aggtgctggc attcgcgata gttcacccgc aagcactcgc cgccccacaa agcgccgttc
    10201 tcccccatac caataccgtc gagcgtcaac gcaatgacat ctccgccatc cagaggccag
    10261 aggtgttctg ccagacacgc cgctgcgtgg gcatgatgat gcagcaccgt ttgcgtcggc
    10321 agattcatct cacgcgccca ctggctggag acatagcccg gatgcgcgtc atgcacaacg
    10381 tattgcggag tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc
    10441 tggatgccat cgtcacttaa atcgcccaga tgctgactca acaccgcttg ttcgccgcgc
    10501 accaggcaga aggtattttt cagatccgcg ccgagacaca gcacaggcgg aacattttta
    10561 aagcccggag gcaaagccag cgcatccggc acataccccc gcgaacggcg cagcatttcg
    10621 ccgctttcgc gcaccaccga atcatccatc cgctgcacga tgtcgcggtt atgtatcaag
    10681 aatccgtcgg caatgccatg caaatccgcc agcgcctgtt cgttgctgat agccggtggt
    10741 ttaccgctca ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa
    10801 tgctggagcg ggttcgcagg caacattacc ccgacttcgt taaggtcagg ggcgatatca
    10861 tcacaaagct caggtacata ttttttatcc accagcacaa tcggcgcggc gggcgtggta
    10921 agcaactggc gcgcagcgtc tggtaaaccg tcagccactg gcaacatgac cgccagcggt
    10981 ttcgccgggc gatgtttgcg cgcccgaagt gtcgccaccg cgttactgtt acgtgcatcg
    11041 caggcaagat gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt
    11101 gcgatagctg cctgtaatgc cgcctcttgt tctgcatgtt cgccatgact tacccattca
    11161 agatgggggc cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg
    11221 tcacagtact ctttgtcaca agccggacac agcggaaacg ccgccatcac ggtaaacggg
    11281 cggtcgtaag gcatggcgcg aataatggtg aaacgcggac cacagtgagt acagttgata
    11341 aacggataac gccagcgtcg ttcgcctggg gtattcattt cggcaagaca agcagggcaa
    11401 gtcgcggcat cgggaacaat ttgcgtattc atgacgccac ccgcgctctg gcggatggtg
    11461 aactcagtgg gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc
    11521 agtggcgggc agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg
    11581 acttctacgc catcgccgtc attacagaca tcgccgtgaa gatttaattg ctgtgccagt
    11641 tgccagacaa acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca
    11701 ccgcaagatg tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc
    11761 cagccggaca cgacagcgtc gcatccggca atcacaggtc ggcgatactg cccgcttcgt
    11821 attctacgaa tatttccggg aattcctttg ataccagaac agttctgtaa gatttttaga
    11881 acatcagcgc cgtgcggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga
    11941 cacaaatcag cgcatgggtc gggcaagccg ccatacacgc cgggccgtct tcacgatggt
    12001 tgcacaggtc gcatttattg gcttcggctt tgtcagcccg tacattcagc cctgcgccgc
    12061 tgttgcgaat caccggacgc accaccactt ccatcgcacc atacgggcaa gccacaacgc
    12121 aggttttgca accaatacaa cgttcctgca tgacatgaac aaacccttta tcacggctga
    12181 tagcaccatt cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg
    12241 ccgtggaaat gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca
    12301 gcgatgcaca gtcctgattt tcctgatgag aaaccacgca cgctacttca caggtacggc
    12361 aaccaataca tttactcgcg tcagcaatga tgaaacggtt catcaaattc tccagcaata
    12421 acagttaatg cgccgataca ttcacaaatc atgccagttt ttaatttact gttatttaag
    12481 gaaattaaat tctgtactac aggaaaaacg atgtcatcga cactagtgac gatgacatgt
    12541 gatgacaatg tttatcgcga aagagcaatg agtgagccgc ggcggattag ttttccgctg
    12601 aaggttttcg gcggtgagaa atccccgcca tcgagcataa aaatcagccg tccaataatt
    12661 tcctgaatca tctcagtcac cggaattttt acgctggaga gcgccggaac gatgtagggg
    12721 gcaatagcga tatcatcgaa tccgataact gacacctgct ctggcaccgc tacgccgcgc
    12781 tcgtgtaacg ctttgatcgc acctatcgcc atatcgtcgt tactggcaac taacgcgcta
    12841 aatttagccc cacgttcgag caacgtttct accccttcgg caccgctggc gggcgtccat
    12901 ttaccgttag cgataagttt ttcattgaac gcaataccat gctgcgccag cgcgtcttta
    12961 tatccggcaa gacgttcaat gctggtgggg gaatccatcg agccggtaag gaaagcaatc
    13021 tcctgatgtc cggcggttat caactctgcc acggcgttaa agctggtctg tttatgatcg
    13081 caccagacgc tatggctgct gtttttgcgc aggcggcgat taagcaccat tatcggctga
    13141 ctgtgtgcgt caatgatgtc atcaatctca tccacgctta aaaagcgcgg gtaaatcatg
    13201 atggcgtcgc agcgcaaatc cagcagatac tgaatcgcct ggcgctcttc ttcggcgcta
    13261 tgtttaccat ctgccaatag caactgtcgc cctttctctt ccgccattcg cgcggcatga
    13321 aacagtaatt cactaaaata aatgccatgg taaagcgtgt tggtcacgac cagccccagc
    13381 gtctgagtac tcttcgccga cagattgcgc gccagcaagt ttggacggta accgctctct
    13441 tctaccgcct gaaacacgcg atctttagtc tcctggctga cgtagccatt acctgaaagc
    13501 acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag catcgtcgtc
    13561 atcattttca tccctttaca cgcaatcaac gcagtgtact gcaccgtttg ccgattgtcc
    13621 ttgcgcaatc ggcgggaaaa atattcaggt gaccggtttc acaaatataa aaaatgaaca
    13681 attaactctc ttgcttattg catgttaact attcatgatt ttgtgaaacc ggtttcccaa
    13741 ttccgtttca gcatcggcat ttttccgtca cgtcgactga taacaactac atctacccta
    13801 ctgataacag gataaaatcc gatggccaaa aattatgcgg cgctggcaag ctcggtgata
    13861 acggcactgg gcggcgttga taacatctcg gcggtcacgc actgtatgac gcgtttgcgc
    13921 tttgttatca aagatgacca gcttatcgat agtccgacgt tgaaaaccat ctccggcgtg
    13981 ctcggcgtgg tacgtagtga caaccagtgt caggtgatta taggtaatac ggtttcgcag
    14041 gcctttcagg aagtcgtcag cctgctgccg ggagacttgc aacccgcgcc tcccgtgggt
    14101 aaaccaaagc tcacgctacg tcgcattggt gcgggggtcc tcgatgcgct gatcggcacc
    14161 atgtcaccgc tgatcccggc gattatcggc ggatcgatgg tcaaactgct ggcgatgatc
    14221 ctcgagatga gcggcgtgct gacaaaagga tcgccgacct taatcattct gaatgtgatt
    14281 ggtgacggtg ctttcttctt cctgccgctg atggtcgcgg cttctgccgc catcaaattt
    14341 aaaaccaata tgtcgctggc gattgccatt gcgggtgtgc tggtacatcc gagttttatt
    14401 gagctgatgg cgaaagcggc cctgggtgaa catgttgaat ttgccctgat tccggtcacc
    14461 gcggtgaaat acacctacac agtgatcccg gcgctggtga tgacctggtg cctgtcatat
    14521 atcgaacgct gggtggacag cattacgcca gcggtgacga aaaacttcct caagccgatg
    14581 ctgattgtgt tgattgccgc accgctggca atcctgctga ttggcccgat tggtatctgg
    14641 atcggtagcg ccatttcggc gctggtttac accattcatg gttatctggg ctggctttca
    14701 gtcgccatta tgggcgcgct gtggcctctg ctggtaatga cagggatgca ccgcgtcttt
    14761 acgccaacca tcattcagac cattgccgaa accggcaaag aagggatggt catgccgtca
    14821 gagatcggcg ctaacctgtc gctgggcggt tcatcactgg cggtggcatg gaaaacgaaa
    14881 aactcggaac tgcgccagac ggcgctggct gcggcagcat cagccattat ggcggggatt
    14941 tccgaacccg cgttatacgg cgtggcgatc cgcctgaaac gtccgcttat cgccagtctt
    15001 atcagcggtt ttatttgcgg cgcggttgcc ggtatggcgg ggcttgccag ccactcaatg
    15061 gcagcgccgg ggctatttac cagcgtgcag ttcttcgatc cggcgaatcc aatgagcatc
    15121 gtctgggtgt tcgccgtcat ggcgctggcg gtcgtgctgt cgttcatcct cacactgttg
    15181 ctcggctttg aggatattcc tgttgaggaa gcgactgccg aggcgcgaaa gcatcagagc
    15241 gtacaaccga ccgtcgccaa agaagtaagt cttaattgag gatgaaaatg tcagtatttc
    15301 cgcaaggttt tttatggggc ggcgcgcttg ccgccaacca gtctgaaggc gcgtaccgtg
    15361 aaggcggcaa agggctgacc accgtcgata tgatcccgca cggcgaacat cgaatggcgg
    15421 tgaaactggg gctggaaaaa cgttttcagt tgcgcgatga cgagttttat cccagccatg
    15481 aggcgacgga tttttatcat cgttataaag aagatatcgc cctgatggca gagatgggat
    15541 tcaaggtttt tcgcacctcg attgcctgga gccgcctatt tccgcagggc gatgaactga
    15601 cgcccaatca gcagggcatt gctttttacc gcgcggtatt tgaagagtgt aaaaagtacg
    15661 gtatcgaacc gctggtcacg ttgtgtcact tcgacgtgcc gatgcatctg gttactgaat
    15721 atggctcctg gcgtaaccgc aagctggtag agcttttcag ccgctacgcc cggacctgct
    15781 ttgaagcatt tgatggtctg gtgaaatact ggcttacctt caatgaaatc aacattatgt
    15841 tgcatagccc gttctccggc gcgggtctgg tgtttgaaga aggggaaaat caggatcagg
    15901 tgaaatatca ggctgcgcat caccagctgg ttgccagtgc gctagccacc aaaatcgccc
    15961 atgaggttaa cccgcaaaat caggtgggtt gtatgctggc gggcggtaac ttctaccctt
    16021 acagctgcaa gccggaagat gtctgggcgg cgctggagaa agatcgggaa aacctgtttt
    16081 ttatcgatgt gcaggcgcgg ggcgcgtatc cggcttactc tgcccgcgta ttccgcgaaa
    16141 aaggggtaac catcgacaaa gcaccgagcg atgatgagat cctgaaaaac accgtcgatt
    16201 ttgtctcttt cagctattac gcctcgcgct gcgcctcggc ggagatgaac gccaacaaca
    16261 gcagtgcggc gaacgtggtg aaatcgctgc gtaaccccta tctgcaggtt agctactggg
    16321 gctgggggat tgatccactc ggtctgcgta tcaccatgaa catgatgtat gaccgttatc
    16381 agaagccgct gtttctggtg gaaaacggcc tgggcgcaaa agatgaattt gctgccaatg
    16441 gcgaaattaa cgacgactat cgcatcagct atttacgcga acatatccgc gcaatgggcg
    16501 aagcgattgc agacggcatt ccgctgatgg gctacaccac gtggggctgt attgatttag
    16561 tttccgcctc tacgggtgaa atgagcaaac gctacggttt tgtctacgta gaccgtgacg
    16621 acgcaggcaa cggcacgctg acgcgcacgc gtaagaaatc gttctggtgg tataaaaaag
    16681 tgattgccag taatggtgaa gatttagagt aagtaactac agtcgcacga accaggtagg
    16741 cccgaaaagc gttgcgcatc gggcaatctg gcgtttgcta tcagttctga ttccctcatc
    16801 aagaacatcc ctgtcctgaa ttccttactg aaaaactact cttcttccgc cgctaactgc
    16861 gcgaagccgc catttccttc ccagccttcc agccgctgat acaccgtttc caccgcatct
    16921 ttaatctgct gagtcatcgg gtagtaaaag ccgacgatat ccggctggat gccaaggaaa
    16981 atcacttcgc caatatcttc tttcaactgg tcgataagat agttcagcgg catgttatgg
    17041 gtagtcatca taaacatctc ggcgatgtca gccgggtcga tgatgcggat ctcgccaggg
    17101 tttagcccca tatccgtggc gtcgacaata agcagccgtg tcgggcgcag ttcgcggata
    17161 gcgacaatgt cgttttccgg tgcgctaccg ccgtcaatca ctacccagtt acctttcggc
    17221 gcggcagcgc acttttccgc cagtagcggg cctgcgccat catcacccat catgctattg
    17281 ccaacacaga gtaaaacgtc agtcacgcag tctcctcacc atcaaataga tggcgttttc
    17341 ctgatgaata tcatgcagca tacttaacag cattgtactc cattccttct gctgcgaagt
    17401 ttgcgctgcc cgggctttat cgaatgcgtt ggccagcatc ggcacatggt taatgtcgat
    17461 gacgatctca ccatatttcg gcacgccttc cattttgcgg cgggcttcgc tgcccgcctc
    17521 cagagtggca atccatgcca gatagtcatc ccacgggcag gtaagcgccg cttccaggca
    17581 atcgataacc ccaaggtggt gaccaatcgc caggctgtaa tagaccacct gctgcgcctc
    17641 ggcgggcgta gcatcgttct catcaatgaa tttacggctc agttgactga acaccacctt
    17701 ttcactcatc ggatacgcgc ctcttcaaca acatgattca gatggctgac aatctcgttc
    17761 agacgcggat cgttttccgc ttccagccag cgtgcaacct gttcttcgcc ctgccctaat
    17821 tgcgtcagga aatcatcggc aatctggcga ccgtaacgat aacccgccag gcggcgtgct
    17881 tcgcgatcca ctttcacgcg cagcggctgc accatatcgc catgcagaat ctccgccggt
    17941 tgttcatcct gctcacccgg cccacgggcg tggattttct gctccagcag gccgagcgcc
    18001 atcgcaaagc cgtacagcgt ggcggcaggc gttggcgggc agccagggat ataaacatcc
    18061 actggaacga ttttatccgt accgccccac acgcagtaga gatcgtggaa gatcccgcca
    18121 ctgttaccgc aggcaccgta ggagatacag attttcgggt ccggcgcgga ctgccacgca
    18181 cgcagcgcag gggatcgcat tgcacgggtg accgcgccgg taaacagtaa aatatccgca
    18241 tgacgcggtg acggaacgac tttaatgccg aagcgttctg catcgaacag cggcgaaagc
    18301 gtggcgaaaa tttcgatttc gcaaccgtta cagccgccgc agtccacgcg ataaacgtag
    18361 gcagaacgtt tgattttttt cagtaacgac gccttcatgc tggcgatgga ttcatccacc
    18421 gtcatcggga ccggaatgcc gttggcgtca cgggggccta ataaattgct catcagatgg
    18481 cctctttcat atggcgagtc agttcaatac ggtcggacgg caccaggcat ttctggcgct
    18541 tacattccgg gcaagtctca aagctttcgc ggtggttttc cgcgcggctg tcaccgttgt
    18601 gcttaagcag cgcaatggcg tagtcgatct ctttctggac ggcgaaagga cgattgcaga
    18661 cgcggcagtt gcacagcgcg aagcgggact gttgcaggaa atcttctttc ttccacaccg
    18721 ccagttcgta ctcttgcgac agtttgatcg ccgctgtcgg gcagacttct tcgcagcgcc
    18781 cacagaagat gcagcgccca aggtcaaact gccacgccag ctcaccagtg gcgaggtcgg
    18841 tttcaaccgt taaggcgttt gacgggcagg cattgacgca agccgcacag ccaatgcact
    18901 gctgcgggtt ctgctctggc ttaccacgga agtttttatc aaccgcaatc ggctccagcg
    18961 gataagacga agtcgccgtg ccggttttga tgactttttt gataaaggta aacatggcga
    19021 ttccttattt cagcggcgag tttttacgct caatgctgta gcgctcaagt tctttgtacg
    19081 gcaccacttt gctcttcttc ttacgtacat cgaccacggt catgcggtcg gtacaggagt
    19141 agcaagggtc caggctaccg ataatcagcg gcgcatcgga aacggtgttg ccgcgcagca
    19201 tataacgcag ggtcggccag ttcgcgtagg tcgccgcacg gcagcgccag cggtacagct
    19261 tctggttgtc gccggtcatg ctccagtgga tatcgtcgcc gcgcggcgct tcggcaaagc
    19321 ccagcgcgaa acggtgcgga atgtaggtaa agccttccac catcagcggg ccgcccggca
    19381 ggttatccag accgtaatcg atcatattca gcgcggtata gacttcgttg atacgcactt
    19441 tcagacggga aataacgtcg cagccctgct cgctgtggac ttccattggc agcaggccat
    19501 aaccaacaaa cgggtgatcg gcacgggtat cacgggcgtg accgctggca cgaaccatcg
    19561 ggccaacgtt actgaagtcg cgagcgattt ccgggtccag acgaccaatg ccgacggtgc
    19621 gttgttccat gttcggtgtg ctcagcagca catccaccag ctcctgcact tcacgacgca
    19681 tctgttgtgc cagctggcgg gtctggatca tgtcgtcttt cagcagatcg cgacgaatcc
    19741 cgccgatcag gttcaggccg taggttttac gtgcaccggt aaggatctct gccattttca
    19801 tggaggtttc acgcacgcgg aagaactgca taaagccgga gtcgaagccg gtaaagtgac
    19861 aggccaggcc gagattgagc agatgcgagt gcaggcgttc tacctccagc agaatggcgc
    19921 ggatcatctg cgcgcgttct ggcacctgaa tacccatcgc gttttccacc gacgtggtat
    19981 aggcggtgct gtgagcaaag ccgcagatcc cgcacacacg gtcagagagg aaggtcactt
    20041 cgttgtaacc catacgggtt tccgccagtt tttccatgcc gcgatggacg tagaacaggc
    20101 ggtagtcggc gtcgataatg ttttcaccat cgacgaacag acggaagtga cccggttcgt
    20161 cagaagtgac gtgcagtgga ccaatcggca cgacgttgtt tttcttgtcg cccagttcgt
    20221 tgatgaactc gtaggtttca gcatcggtag tcggtgccgg acgctgacga taatccatgc
    20281 tgtctttacg cagaggataa agttcatccg gccagtcatc cggcagcacc agacgacgtt
    20341 catccggcag accaaccgga atcaaaccgt acatatcgcg cacttcacgc tcgccccaca
    20401 ccgccgccgg aacgcgcggc gtcacggaag gatactccgg tttgttggcg tcaacttcga
    20461 cgcgaaccgt tacccaacac ttggtgccct tctccatcga cagcacgtaa taaacggcgt
    20521 aatgaccatt cagtttgcgt tcgtcgttac caaacagcac cgacagccaa cccccttgtt
    20581 tgtagtagag aaactccacc acttccggca ggtagttcac cttcacggtg acggttagct
    20641 gatctttggt ctgccaggcg tggtccagca cgacgcccgg aaatgcctca ttcagcgcgg
    20701 cgagataatg ttgacctaat ttttcttcag acatgctcaa actctcttta atcacgccgc
    20761 cagcaaggag acgaacgcta aaaatgcaaa gccaaaacca gcccaggtaa tgcgtggagt
    20821 aatatcaaga cgcaaacggg ccatgctgtt ttcgaacagc gcgataacca gtacgccgac
    20881 caccagtttg acgatggcaa tcaccagcgc cagcagcagc ccaccgacgg tgaaggtttc
    20941 catttgtccc cacggaataa acaccccgac gaacatctgc aacaccacca gctgtttcag
    21001 gctgatgccc catttcatga cgccaaagcc gctgccgcta tactcagaga gcggaccttc
    21061 ctgcaactcc tgctcggctt ccgccaggtc gaacggcagt ttgcccattt cgataaaagt
    21121 ggcgaacgca caggcacaaa gcgccagcac cagcgggatg ctctggctca gtggccagtg
    21181 ataaacggtg tcggtgatgt tgctgatgtt ggtggaaccg gcaacctgtg cggcgaccca
    21241 cagaccaagc agcagcatcg gttcaaccaa cacgccaagc atcgcttcac ggctcgcacc
    21301 gatagcggta aacgggctac cagtatccag accagaaatg gcaaagaaga aacgcgcgat
    21361 ggcgaagaga tacagtaagg tgatcaaatc gcccagtggc ggcagcggag agccgacggt
    21421 caccaccggc agcgcagtgg cgatagtcag catgacgccc accatcacat acggcgtcag
    21481 gcggaacacc cagccggagg catccgggcc aacgctctgg cggcccagca gtttgataat
    21541 gtcgcgatac tcctgcaaca cacccggccc acgacggtta tgcagacggg cgcgcgctac
    21601 gcgggtaata ccggagagca gcggcgcaac ggcaaatagc accagcgcct gaattaacgg
    21661 atataaaaca ctcattctca ggctcctcgt gaaacaataa tcaccaccag caccgccagt
    21721 tcaaccagcg ccatccggcg gaacagcaac gcactcccct cgcactgcca gcccggcacc
    21781 agagacaccg gattcagcca tttgcgtagt ttcagcaccg gcgcaaacgc ctgtttcacc
    21841 ggcatggcaa aaccgtgagc ggtaatcacc attgattttt catgatcgta gccgcacacc
    21901 caggccgcac cgcgggaacg cgacggcaaa cgatcgcctt tgcaaatcgc cataatgatg
    21961 aatggcagca gcgggcaggc aatcagcagc aacgtgatca ttggttgaga aacggtggtg
    22021 ttagcaggct ccagcggcag aggtacagca gcagagagca tcggcagtag ccacggcgca
    22081 gcaacaccgc caattacgca gcaaatcgcc agtgccacta cgcttacgct catcaggagc
    22141 ggcgcacagg tggcgttttc ggcctctttg gtacgcggcg cgccgaggaa agtaacgcca
    22201 taaactttcg ccatacacat caccgccagc gcaccggtaa ttgccagccc cacggcgagc
    22261 agcggcccga gaagacgagc aacaaacgcg ccactattgc tcagtttgaa gaaggattga
    22321 tagataaccc attccccggc aaaaccgttc agcggcggca gcgcagccat tgccatcagc
    22381 ccgactaaca tggcgatgga gataaccggc attttcttgc caataccacc gagtttttcg
    22441 atatcgcgat gaccggtacg gaaccagacg ctccccgcac caaggaacag tacgctcttg
    22501 aacaggctat ggttaagcag atggtacaga ccaccaacca gaccaagggc aatcagcgcc
    22561 ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc ccagcaggat aatgccgata
    22621 ttctccaggg tgtggtaagc cagcagacgt tggatattat gttccatcaa cgcatacaga
    22681 ccgccgacga atgcggtgat cataccgagc accagcagcg cgatccccca ccacagcggt
    22741 gcattaccgc ccagtagtga cagagttaaa atgcccagca ggccaatttt catgactacc
    22801 gtggaaaaca gcgcggcagc tggcgcagag gcgttggcat gtgcctgtgg cacccagccg
    22861 tgcagcggaa taatcccggc cagcaggcca aagccaatca caccgagcag ccagatatcg
    22921 gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccgtaa
    22981 cgctgccaca gcagccagca ggcaatcgcc agcagcagag tgccaagacg ccccagcgca
    23041 aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacag ggccatgatt
    23101 tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcacagac ggcggcagcc
    23161 atcaacatat tgatctgcaa gccgttgcat ttcacctgcg cgtggcgatg ccagtcaatg
    23221 ttgtagaggc tgacaaacag accgcatagg ccgagtgtaa tcagccaaat cgcgttaagc
    23281 ggagagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc aaccgtgcca
    23341 gtcagcacag tgaagcccgc ggctgccgta tacaggctac ccaccgcgcc gccaattccg
    23401 gctatccagc cacttagcgc tttttgaaaa gaaaagagaa atgccagaac agcgacggcg
    23461 acaaaccacg ccacgccgct attgatcagg gaaattgcgc tcatttagcc tctccacttt
    23521 gagcctgctg aaacaaggtg agatcgccaa agtccgtgtt aaaagtcagc tcacgcttac
    23581 gtttgctgac gcgggcgata tcggtgttat ccaccagatg cagggcttta gtcgggcaca
    23641 tccgcacgca ggccggacct tgttcatcaa agctacaaag gtcacatttg acggcgatcg
    23701 cgcgaatacc tggcacccag tcaagcaatg tgctgacacg cgccggagca ggcggtgccg
    23761 gtggcgcttt cggggtattg gcgtttgccg gaatatccag cggacggctg ccggaaaatt
    23821 caattgcgcc aaacgggcag gcgatgccac acagtttgca gcttacgcac aagctttcat
    23881 tcagctgcac ggcaccatca acacgagtga tggcgttaac cgggcagacc gttgcgcagg
    23941 gtgcatcttc acagtggtga cagagctgcg gcgcagattc tttttcattt agcatcactc
    24001 tcaggcgcgg cattgattgc aggccgtgct ggcgatgcgt ctctgaacag gcagcctcac
    24061 aagtgtggca gccgatacag agcgtggagt cagcaattac aaaacgattc accaggcatt
    24121 cctcaggtga ttgtcatttt tgacgaaaac atgccgatga aatgtcattt tcgacactca
    24181 tcgacacgcc catccccaaa caggcgtaac gcctgcaaaa cgggcaaagc ctcagctcat
    24241 gctgccgggc tttgtcccgt taccagttgg cttaaattca ccggcacatt gttttcaatg
    24301 gcggtgtata agctgtcgta aacgccagcg attttttcga gatagtgttc cagaacctgt
    24361 tcgcgatcgc ggatgttgac cttgccatca atcatgccgt caatgctcag ttgcgcctgc
    24421 gcaatccgct gtttgtcttc accctctttc gtttcgacgt aatactcgat ggtgtggctg
    24481 ttaaagccat ccgccagggt gacgtaaatg cgaaaatgtt caaaaaggac gaaacagagt
    24541 tctttgtccg gcgcgcagct catggcgtga tgcaggcgcg ctttcgataa cgtgatcccc
    24601 tgaaccagcg aattgcagta gatgtgccac tggtcctgta ggcgacgatg ccgctgtgcg
    24661 atgtaatcgg ctttctcgct tatttcccaa atagtcattg tcaggttacc cgtttaacag
    24721 agatgccagc tttaagcatt ttctgtgcca acttttaatt tattgttatt aaagcgattt
    24781 ttaagctaaa gatgaatttc gtcgccgtgt cgacgtgtca tttcgacatc atcgacatta
    24841 tttaccgcag ggataatcaa cactggcaca attattgctt gtagctggca atagttaatg
    24901 ggaggcgata tgcacgaaat aaccctctgc caacgggcac tggaattgat cgaacagcag
    24961 gccgcaaaac acggcgcaaa acgcgtaact ggggtctggc tcaaaattgg cgcattttct
    25021 tgtgtcgaaa ccagctctct tgccttttgt tttgatctgg tttgccgcgg cagcgtggcg
    25081 gaaggttgta aactgcacct cgaagaacaa gaggccgaat gctggtgtga aacatgccaa
    25141 cagtatgtga cgctactgac ccagcgcgtc cgccgctgtc cacagtgtca tggtgacatg
    25201 ctacagattg tggcagacga cggtttacag attcggcgga tagaaataga ccaggagtga
    25261 gcgatgtgta caacatgcgg ttgcggtgaa ggcaacctgt atatcgaggg tgatgaacat
    25321 aaccctcatt ccgcgtttcg tagcgcgcca tttgccccgg cggcacgccc gaagatgaaa
    25381 atcaccggca ttaaagcgcc tgaatttacc cccagccaga ctgaagaagg cgacctgcat
    25441 tacggtcatg gcgaagcggg cactcacgcg ccgggcatga gccagcgtcg gatgctggaa
    25501 gtcgaaattg acgtgctgga caaaaataac cgtctggctg aacgcaaccg cgcgcacttt
    25561 gctgcccgca agcaactggt gctcaacctg gtttccagcc ctggttccgg taaaaccacc
    25621 ctgctgacgg aaaccttaat gcgcctgaaa gacagcgttc cgtgcgcagt tattgaaggc
    25681 gaccagcaaa ccgtgaacga tgccgcgcgc attcgtgcta ccggcacgcc agcgattcag
    25741 gtgaacaccg gtaaaggctg ccatcttgac gcacagatga ttgccgacgc cgcgccgcgc
    25801 ctgccactgg acgataacgg tattctgttt atcgaaaacg tcggcaacct cgtgtgcccg
    25861 gccagcttcg atctcggtga aaaacacaaa gtggcggtgc tttccgttac cgaaggtgaa
    25921 gacaaaccgc tgaaatatcc gcatatgttt gccgccgcct cgctgatgct gctcaacaaa
    25981 gttgacctgc tgccgtatct caactttgac gttgagaagt gcatcgcctg cgcccgtgaa
    26041 gtcaatccag aaattgaaat catccttatt tccgccacca gcggcgaagg gatggaccag
    26101 tggctgaact ggctggagac acagcgatgt gcataggcgt tcccggccag atacgcacca
    26161 ttgacggcaa ccaggcgaaa gtcgacgtct gcggcattca gcgcgatgtc gatttaacgt
    26221 tagtcggcag ctgcgatgaa aacggtcagc cacgcgtggg ccagtgggta ctggttcacg
    26281 ttggctttgc catgagcgta attaatgaag ccgaagcacg cgacactctc gacgccttac
    26341 aaaacatgtt tgacgttgag ccagatgtcg gcgcgctgtt gtatggcgag gaaaaataat
    26401 gcgttttgtt gatgaatatc gcgcgccgga acaggtgatg cagttaattg agcatctgcg
    26461 cgaacgtgct tcacatctct cttacaccgc cgaacgccct ctgcggatta tggaagtgtg
    26521 tggcggtcat acccacgcca tttttaaatt cggcctcgac cagttgctac cggaaaacgt
    26581 tgagtttatc cacggtccgg gttgccctgt gtgcgtactg ccaatgggta gaatcgacac
    26641 ctgcgtggag attgccagcc atccggaagt catcttctgt acctttggcg atgccatgcg
    26701 cgtaccaggg aaacaggggt cgctgttgca ggcaaaagca cgcggtgccg atgtgcgcat
    26761 cgtttactcg ccaatggatg cgttgaaact ggcgcaggag aatccaaccc gtaaagtggt
    26821 attcttcggc ttaggttttg aaaccaccat gccgaccacc gccatcactc tgcaacaggc
    26881 gaaagcccgc gatgtgcaga atttttactt cttctgccag cacattacgc ttatcccgac
    26941 gctgcgcagt ttgctggaac agccagataa cggtatcgac gcgttcctcg cgccgggcca
    27001 cgtcagtatg gttatcggca ctgatgccta taattttatc gccagcgatt ttcatcgtcc
    27061 gctggtggtc gctggtttcg aaccccttga tctacttcaa ggcgtggtga tgctggtgga
    27121 gcagaaaata gcggcccaca gcaaggtaga gaatcagtat cgtcgggtgg taccggatgc
    27181 cggtaacctg ctggcgcaac aggcaattgc tgatgtgttc tgtgtcaatg gcgacagtga
    27241 atggcgcggc ttaggcgtga ttgaatcttc tggcgtgcac ctgacgccgg attatcaacg
    27301 attcgatgcc gaagcacatt tccgcccggc accgcagcag gtctgcgatg acccgcgtgc
    27361 gcgttgtggc gaagtcttga cgggcaaatg taagccgcat caatgcccgc tgtttggtaa
    27421 cacctgtaat cctcaaaccg cgtttggtgc gctgatggtt tcctccgaag gagcgtgcgc
    27481 cgcgtggtat cagtatcgtc agcaggagag tgaagcgtga ataatatcca actcgcccac
    27541 ggtagcggcg gccaggcgat gcagcaatta atcaacagcc tgtttatgga agcctttgcc
    27601 aacccgtggc tggcagagca ggaagatcag gcccgtcttg atttggcaca gctggtagcg
    27661 gaaggcgacc gtctggcgtt ctcaaccgac agttacgtca ttgacccgct gttctttcct
    27721 ggcggtaata tcggcaagct ggcgatttgc ggcaccgcca atgacgttgc ggtcagtggc
    27781 gcaattccgc gctatctctc ctgtggcttt atcctcgaag aaggattgcc gatggagaca
    27841 ctgaaagccg tagtgaccag catggcagaa accgcccgcg cggcaggcat tgccatcgtt
    27901 actggcgata ccaaagtggt acagcgcggc gcggcagata aactgtttat caacaccgct
    27961 ggcatgggcg caattccggc gaatattcac tggggcgcac agacgctaac cgcaggcgat
    28021 gtattgctgg ttagcggtac actcggcgac catggggcga ctatccttaa cctgcgtgag
    28081 caactagggc tggatggaga actggtcagc gactgcgcgg tgctgacgcc gcttattcag
    28141 acgctgcgtg acattcccgg cgtgaaagcg ctgcgtgatg ccacccgtgg tggtgtaaac
    28201 gcggtggttc atgagttcgc ggcagcctgc ggttgtggta ttgaactttc agaagcggca
    28261 ctgccggtta aacctgctgt gcgcggcgtt tgcgaactgc tgggactgga cgcccttaac
    28321 tttgccaacg aaggcaaact ggtgatcgcc gttgaacgca acgcagcaga gcaagtgctg
    28381 gcggcgttac attcccatcc attgggaaaa gatgcggcgc tgattggcga agtagtagaa
    28441 cgtaaaggtg ttcgtcttgc cggtctgtat ggcgtgaaac gaaccctcga tctaccgcac
    28501 gccgaaccgc ttccgcgtat atgctaataa aattctaaat ctcctatagt tagtcaatga
    28561 ccttttgcac cgctttgcgg tgctttcctg gaagaacaaa atgtcatata caccgatgag
    28621 tgatctcgga cagcaagggt tgttcgacat cactcggaca ctattgcagc agcccgatct
    28681 ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt tctgcgctcg ccgacaacgc
    28741 ggcgattgtg ttgtggcaag cgcagactca acgtgcgtct tattacgcat cgcgtgaaaa
    28801 agacaccccc attaaatatg aagacgaaac tgttctggca cacggtccgg tacgcagcat
    28861 tttgtcgcgc cctgatacgc tgcattgcag ttacgaagaa ttttgtgaaa cctggccgca
    28921 gctggccaca ggtgggctat accccaaatt tggtcactat tgcctgatgc cactggcggc
    28981 ggaagggcat atttttggtg gctgtgaatt tattcgttat gacgatcgcc cctggagcga
    29041 aaaagagttc aatcgtctgc aaacatttac gcagatcgtt tctgtcgtca ccgaacaaat
    29101 ccagagccgc gtcgttaaca atgtcgacta tgagttgtta tgccgggaac gcgataactt
    29161 ccgcatcctg gtcgccatca ctaacgcggt gctttcccgc ctggatatgg acgaactggt
    29221 cagcgaagtc gccaaagaaa tccattacta tttcgacatt gacgatatca gcatcgtctt
    29281 acgcagccac cgtaaaaaca aactcaacat ctactccact cactatcttg ataaacagca
    29341 tcccgctcac gaacagagcg aagtcgatga agccggaacc ctcaccgaac gcgtcttcaa
    29401 aagtaaagag atgctgttga ttaatctcca cgagcgggac gatttagccc cctatgaacg
    29461 catgttgttc aacacctggg gcaaccagat tcaaaccttg tgcctgttac cgctgatgtc
    29521 tggcgacact atgctgggcg tgctgaaact ggcgcaatgc gaagagaaag tctttaccac
    29581 taccaatctg aatttactgc gccagattgc cgaacgtgtg gcaatcgctg tcgataacgc
    29641 cctcgcctat caggaaattc atcgtctgaa agaacggctg gttgatgaaa acctcgccct
    29701 gaccgagcag ctcaacaatg ttgatagtga atttggcgag attattggcc gcagcgaagc
    29761 catgtacagc gtacttaaac aagtggaaat ggtggcgcaa agtgacagta ccgttctgat
    29821 cctcggtgaa actggtacag gtaaagagct gattgcccgc gcgatccata atctcagtgg
    29881 gcgtaataat cgccgcatgg tcaaaatgaa ctgcgcggcg atgcctgccg gattgctgga
    29941 aagcgatcta tttggtcatg agcgtggggc ttttaccggt gccagcgctc agcgcattgg
    30001 tcgttttgaa ctggcggata aaagctcact attcctcgat gaagtgggcg atatgccgct
    30061 ggagttacag ccgaagttgc tgcgtgtatt acaggaacag gagtttgaac gtctcggcag
    30121 caacaaaatc attcagacgg acgtacgttt aattgccgcg actaaccgcg atctgaaaaa
    30181 aatggtcgcc gaccgagagt tccgtagcga tctctattac cgcctgaacg tattcccgat
    30241 ccacctgccg ccactacgcg agcgtccgga agatattccg ctgctggcga aagcctttac
    30301 cttcaaaatt gcccgtcgtc tggggcgcaa tatcgacagc attcctgccg agacgctgcg
    30361 catcctgagc aacatggaat ggccgggcaa cgtgcgtgag ctggaaaacg tcatcgagcg
    30421 cgcggtattg ctaacacgcg gcaacgtgct gcagctgtca ttgccagata ttgctttacc
    30481 agaacctgaa acgccgcctg ccgcaacggt tgtcgcccag gagggcgaag atgaatatca
    30541 gttgattgtt cgcgtgctga aagaaacaaa cggcgtggtt gccgggccta aaggcgctgc
    30601 gcaacgtctg gggctgaaac gcacgactct gctgtcacga atgaagcgac tgggaattga
    30661 taaatcggca ttgatttaac agatgtggtt atactcgatc aacattcaat gaaaggaggc
    30721 agtatggcag tttcagcaag aaatcaatta accggaacag ttagcgcagt agcaatgggc
    30781 gcggtgaatg atgaagtaga actgacgctg gcaggtggtg caaagctggt ggcaattgtg
    30841 actcacagca gccagcaagc gctgggtctg gcgaaaggga aagaagcgat tgccctgatt
    30901 aaagccccgt gggtcacgct ggcaacggaa gactgtggac tgaaattctc tgctcgcaac
    30961 cagtttgctg gcagcgtgtc gacaatcacc gaaggcgcag tgaacgccac tgtccatatc
    31021 aaaacggatg cgggttttga aattgtggca gtggtaacaa atgaaagtca ggatgaaatg
    31081 aaactcacca ctggctcgcg cgttattgca ttgatcaaag catcagccat cctgattgcc
    31141 accaaagcgt aagcgccgta tttgccggag agtatggctc tccggcagat ttatttcttt
    31201 ggacgatatt tttctggcaa ctccggcacc ggacgcttgt catcgatgag atgacgcacg
    31261 gttaagatcg gatgacgcca cagcattctc ggcccaaccc aacgcataat ctgtttcatc
    31321 tcttcacgct ttgcaggctg gtaacagtgc accggacact gcttacaggc tggtttctct
    31381 tcgccgaaca cacatttatc cagccgcttt tgcgcgtaag caaacaacgc ctcgtaatgc
    31441 tccggctctg ctgacgcctg cgggcatttc gcttgataaa gatcgatcat ttttttaatc
    31501 gtcagttttt cacgagagat acgcttgccg gacatgctgc ctccatactc ttaaggtgca
    31561 tttatattac aacttaattt taaaggggac tatgtctctg aaggaggagg tttcttcaac
    31621 cgacgaattt ggcgatcttg tttcatcgtc atcacgcaaa agctgcaaag cagcattttt
    31681 cgcggaaccg acatcaagaa ctcacctttc gcgtcttccc ctgaaatgat taactccggt
    31741 atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacactac atttaacatc
    31801 agggagccgg acttaacccc atgagtacaa tagaaaattt cgacgcccat acgcccatga
    31861 tgcagcagta tctcaagctg aaagcccagc atcccgagat cctgctgttt taccggatgg
    31921 gcgactttta tgaactgttt tatgacgacg caaaacgggc atcgcaactg ctggatattt
    31981 cactgaccaa acgcggtgct tcagcgggag agccaatccc gatggcgggg attccctacc
    32041 atgcggtgga aaactacctc gccaaactgg tgagtcaggg cgaatccgtc gctatttgtg
    32101 aacagattgg cgatccggcg accagcaaag ggccggttga gcgcaaagtt gtccgtatcg
    32161 ttacgccggg caccatcagc gatgaagcgc tgttgcagga acgtcaggac aacctgctgg
    32221 cggctatctg gcaggacagt aaaggtttcg gctacgcaac gctggatatc agttccggtc
    32281 gttttcgcct gagcgaaccg gcagaccgcg aaacgatggc ggcagaactg caacgtacga
    32341 atccagcgga attactgtat gcggaagatt tcgccgagat gtcgctgatt gaaggtcgcc
    32401 gcggcctgcg tcgtcgcccg ctgtgggagt ttgaaatcga caccgctcgc cagcaattga
    32461 acctgcaatt tggcacccga gatctggtcg gttttggtgt ggagaacgca ccacgcggac
    32521 tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg accctgccgc
    32581 atattcgttc tatcactatg gaacgtcagc aggacagcat cattatggat gccgcgacgc
    32641 gtcgtaacct ggaaattact cagaacctgg ccggcggtgc ggaaaatacg ctggcttcag
    32701 tgctcgactg cactgtaacg ccgatgggca gccgtatgct gaaacgctgg ctgcatatgc
    32761 cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca ttgcaggatt
    32821 tcaccgctga gctacagccg gtactgcgtc aggtcggcga cctggaacgt attctggcgc
    32881 gtctggcgtt gcgtaccgct cgcccgcgcg atctggcccg tatgcgtcac gctttccagc
    32941 aactgccgga gttgcgtgcg cagttagaaa atgtcgatag tgcaccggta caagcgctgc
    33001 gtgagaagat gggcgagttt gccgagctgc gcgatctgct ggagcgagca atcatcgaca
    33061 caccaccggt gctggtacgc gacggtggtg ttatcgcatc gggctataac gaagagctgg
    33121 atgagtggcg cgcgctggct gacggcgcga ccgattatct ggagcgtctg gaagtccgcg
    33181 agcgtgaacg taccggcctg gacacgctga aagttggctt taatgcggtg cacggctact
    33241 acattcaaat cagccgtggg caaagccatc tggcaccgat taactacatg cgccgccaga
    33301 cgctgaaaaa cgccgagcgt tacattattc cggagctgaa agagtacgaa gataaagttc
    33361 tcacctcaaa aggcaaagca ctggctctgg aaaaacaact ttatgaagag ctgttcgacc
    33421 tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg gaactcgacg
    33481 tgctggttaa cctggcggag cgggcctata ccctgaacta cacctgcccg acctttattg
    33541 ataaacctgg cattcgtatt accgaaggtc gccatccagt ggttgaacag gtactgaacg
    33601 aaccgtttat cgctaacccg ctaaacctgt cgccacagcg ccgcatgttg atcattaccg
    33661 gtccgaacat gggcggtaaa agtacctata tgcgccagac cgcgttgatt gcgctgatgg
    33721 cgtatatcgg cagctacgta ccggcgcaaa aagtcgagat tggcccgatt gaccgtatct
    33781 ttacccgcgt aggtgccgcg gatgatctgg cttccgggcg ttcaaccttt atggtggaga
    33841 tgaccgaaac tgccaatatt ttacataacg ccaccgaata tagtctggtg ttgatggatg
    33901 agattgggcg tggaacgtcc acttacgatg gtctgtcgct ggcgtgggcg tgtgcggaaa
    33961 atctggcaaa taagatcaaa gcgttgacgc tgtttgctac ccactatttc gagctgaccc
    34021 agttaccgga gaaaatggaa ggcgtcgcca acgtgcatct cgatgcactg gagcacggcg
    34081 acaccattgc ctttatgcat agcgtgcagg atggcgcagc gagcaaaagc tacggcctgg
    34141 cggttgcagc gctggcaggc gtgccaaaag aggttattaa gcgcgcacgg caaaaactgc
    34201 gtgagctgga aagcatttcg ccgaacgccg ctgctacgca agtggatggt acacaaatgt
    34261 ctttgctgtc cgtaccggaa gaaacttcgc ctgcggtcga ggcactggaa aacctcgacc
    34321 cggattcact gactccgcgt caggcgctgg agtggattta tcgcttgaag agtctggtgt
    34381 aataataatt cccgatagcc ttttgctatc gggaatatta acgacaactg acgaatcaaa
    34441 taaaaatacc ctgtataata ggaaaactta ttttacaggg taaaaccatg ccatctacgc
    34501 gctatcaaaa aatcaatgcc catcactatc gccatatatg ggccgttggt gatattcatg
    34561 gtgactatca attattacaa tcccgcttac atcaactctc tttttgtcct gaaaccgact
    34621 tacttatttc tgtcggcgat aacattgatc atgggccgga gagtcttaac gtgctgcgcc
    34681 tgttaaacca gccctggttt acatcggcta aaggcaacca cgaagcgatg gcgcttgagg
    34741 cattcgaaac tggcgatgga aatatgtggc ttgccagcgg tggtgactgg tttttcgatt
    34801 taaatgattc agagcaacaa gaggcaatag atctgttgct gaaattccat caccttccac
    34861 atattattga aatcactaac gacaccataa aatatgtcat cgcacatgca gattatccgg
    34921 ggaaggagta tcagtttggc aaagaaatag cggagagcga attactctgg cctgttgatc
    34981 gtgtgcagaa atcacttaat agcgaattac aaaaaataaa cggcgccgac ttctttatat
    35041 ttggtcatat gatgtttgat aacattcaga cgttcgctaa ccagatttat atagataccg
    35101 gatcgccgaa aagcgggcgg ctgtcatttt ataaaataaa gtagtctcat gcttcttctg
    35161 tgaagcatga gtaacccggc gttattgcag gtcattattc atttttcgct accagcaaag
    35221 agagatccag cttcgccagc gcgcggctgg cactctccgg cagaccatcg tcagtaatga
    35281 tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc cacctgtcca tatttcgtcg
    35341 catcacagac caaaactcgc tggcggctgg cactggcaat cgcccgtttc accgtgactt
    35401 tatcttctgc tggcgtagag atcccccgca cactccatga cgatgcagaa ataaaagcct
    35461 gatcaatcat caggctacgc agcatggtcg cagcggcttc cccaacacag gaacggtttt
    35521 cccgacacac tgcgccgcca gtgtgaataa ttgtgcaatt actgttgtcg agcaagtagt
    35581 ccgcaataac gaagtcgtta gtaaccactg tgagtggctc catgtgagtc agatgctgtg
    35641 caatcgctaa cgtggtcgtt cccgcatcca gatagataca acgtcccggc tgaacaagac
    35701 ttgccgccag cttgccaata gccgcttttt gcgtcattgc cagcgcagtt tttacctgat
    35761 gagaaggttc atgtgccacg cgtcccggag actggacgcc cccggacacc aacacaacgg
    35821 ctccctgctg ctccagtttt tgtaaatccc gacgaatggt catatgtgac acattcattc
    35881 tgtccgttag ttcagcaata ctaacaatgc ctttttcagc taccatctca aggatgattt
    35941 ggcgacgctc tacgggtatc aacttttgct ccttcctttg tcctgctgac attctacgct
    36001 atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt tcacataaaa taacacgcaa
    36061 tgttaatttt tgtgaatcaa ctcaccatat cgttatcttt ccgcgcttat attcacaaca
    36121 tcaaacaaaa tatcacttaa attaacaagg agagcagatg aaaacgggat cggagtttca
    36181 tgtcggtatc gttggcttag ggtcaatggg aatgggagca gcactgtcat gtgtccgcgc
    36241 aggtctttct acctggggcg cagacctgaa cagcaatgcc tgcgctacgt tgaaagaggc
    36301 tggtgcttgc ggagtttctg ataacgccgc gacgtttgcc gaaaaactgg acgctctgct
    36361 ggtgctggtg gtcaatgcga cccaggttaa acaggtgctg tttggtgaaa aaggcgttgc
    36421 acaacatctg aaacccggta cggcagtaat ggtttcttcc actatcgcca gtgctgatgc
    36481 gcaagaaatt gctaccgctc tggctggatt cggtctggaa atgctggatg cgccggtttc
    36541 tggtggtgcg gtaaaagccg ctaacggtga aatgactgtc atggcctccg gtagcgatat
    36601 tgcctttgaa cgactggcac ccgtgctgga agccgttgcc ggaaaagttt atcgcatcgg
    36661 ttcagaaccg ggactgggtt cgaccgtaaa aattatccac cagttgttag cgggcgtaca
    36721 tattgctgcc ggagccgaag cgatggcgct tgcagcccgt gcggggatcc cgctggatgt
    36781 gatgtatgac gtcgtgacca atgccgccgg aaattcatgg atgttcgaaa accggatgcg
    36841 tcatgtggtg gatggcgatt acaccccgca ttcagccgtc gatatttttg ttaaggatct
    36901 tggtctggtt gccgataccg ccaaagccct gcacttcccg ctgccattgg cctcaacagc
    36961 attgaatatg ttcaccagcg ccagtaacgc gggttacggg aaagaagacg atagcgcagt
    37021 tatcaagatt ttctctggca tcactctacc gggagcgaaa tcatgatcaa gattggcgtt
    37081 atcgccgatg attttaccgg cgcgacggat atcgccagtt ttctggtgga aaacggtcta
    37141 ccaacggtac aaattaacgg tgttccaaca ggtaaaatgc cggaagcaat cgacgcactg
    37201 gtgatcagcc tgaaaacgcg ctcctgtccg gtggttgaag ccacacagca atcgctggcg
    37261 gctctgagct ggttgcaaca gcaaggttgc aaacagatct atttcaaata ctgctctact
    37321 ttcgacagta cggcgaaagg gaatatcggc ccggttaccg atgcattaat ggatgctctc
    37381 gacacgccgt ttacggtctt ctctccggcc ctgccggtca acggacgtac ggtttatcag
    37441 gggtatttgt tcgtaatgaa tcaactgctg gccgaatccg ggatgcgcca tcacccggta
    37501 aatcccatga ccgacagcta tcttccccgt ctggttgaat cgcaatccac agggcgctgc
    37561 ggcgtcgttt cagcacatgt tttcgaacaa ggtgtgaaag ccgttcgtca ggagctggct
    37621 cgcttacagc aagagggcta tcgctacgcg gtgcttgatg cgctgaccga acaccatctg
    37681 gaaattcagg gggaagcctt gcgcgatgcc ccactggtaa cgggcggttc tggtctggcc
    37741 attggcctgg cccggcagtg ggcgcaagaa aacggtaacc aggctcgcga agcagggcgt
    37801 ccgctcgctg ggcgcggcgt agtgctctcc ggttcatgct ctcaaatgac caaccgccag
    37861 gtggcacatt accgtcaaat tgcaccagcc cgtgaggttg atgtggcacg ctgcctctca
    37921 accgaaactc tagccgctta tgcacacgag ctggcagagt gggttctggg ccaggaaagt
    37981 gtacttgctc cactggtttt tgccaccgcc agcactgacg cattggcagc aattcaacag
    38041 caatacggtg cacaaaaagc cagtcaggca gtggaaacac tgttttctca actagcggcg
    38101 cggttagcag cggaaggcgt gacacgcttt attgtcgcag gcggtgagac ctccggcgta
    38161 gtcacacaga gcctgggcat aaaaggtttt catattggcc caaccatttc ccccggcgtg
    38221 ccgtgggtaa acgcactgga taagcctgtc tcactcgccc ttaaatctgg caacttcggt
    38281 gatgaagcct ttttttcacg agcccaaaga gagtttttat catgagcgat ttcgcaaaag
    38341 tagagcagtc tttgcgagag gagatgacgc ggattgccag ttcattcttt cagcgcggat
    38401 atgcaactgg ttcggctggc aatctgtcgc tgcttttacc tgacgggaat ttactggcga
    38461 caccgacagg ttcatgcctg ggcaatctcg atccacagcg gctttccaaa gtcaccgcgg
    38521 atggcgaatg gttaagtggt gacaaaccct cgaaagaggt gctctttcat ctggcgctgt
    38581 atcgcaacaa tccgcgctgt aaagcggtag tgcatttgca cagtacgtgg tcgacggcgc
    38641 tttcctgcct ggaagggctg gacagcaata acgttattcg gccgttcaca ccatacgtgg
    38701 tgatgcggat gggtaatgtc ccgctggtgc cctattaccg accgggcgat aaacgcatcg
    38761 cacaggatct ggcggaactg gcagcagaca atcaggcttt tttactggca aatcatggcc
    38821 cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa tatggaagag ctggaggaaa
    38881 cggcaaagct gatttttatt ctcggtgacc gcccgatccg ttatctgacc gcaggtgaaa
    38941 ttgcggaatt aaggagttaa tgcaatgcct cgttttgcag ctaatttatc catgatgttc
    39001 accgaagtgc cttttattga acgcttcgcc gcagcgcgaa aagccgggtt cgatgctgtg
    39061 gagtttctgt ttccctatga ctactccccc cagcaaatcc agcagcaact ggtgcaaaac
    39121 cacctgacgc tggcgctgtt caataccgct cccggagata ttaatgcggg agaatgggga
    39181 ttatccgccc ttcccggacg tgagcacgaa gcacgcgcag acatcgacct ggcactggaa
    39241 tatgcattgg cgctcaattg tgaacaagtc catgtgatgt caggcgtcgt gcccgctggc
    39301 gaagatgctg aacggtaccg ggcagtattt attgataatc tccgctacgc tgccgaccgc
    39361 tttgcgccac acggcaagcg aatattggtt gaagcactca gccccggcgt taagcctcat
    39421 tatctcttct ccagccagta tcaggcactg gctattgttg aagaggttgc gcgagataac
    39481 gtgtttattc aactagatac tttccatgca caaaaagtag atggcaacct gacacattta
    39541 atccgcgact acgccgggaa atatgcgcac gtacaaattg ccggactacc tgatcgacat
    39601 gaaccggacg atggagaaat caactatccg tggctgttcc gcctgttcga tgaagtggga
    39661 tatcagggct ggatcggttg tgaatacaaa cctcgtggcc tcaccgaaga agggcttggc
    39721 tggtttgacg cctggcgcta attcgctcaa tccctaatta caacgtaccc atacatcccc
    39781 ccatctacgg atggggtagg ttaactataa ctttcagaca gggtttccca tgtccacaat
    39841 tacattgtta tgcattgcgt tagctggcgt aatcatgctg ttgctactgg tcatcaaggc
    39901 aaaggtacaa ccattcgttg ctctgctcct cgtcagcctg ttagtcgcac ttgcggcggg
    39961 tataccggcg ggcgaagtgg gtaaagtgat gatcgccggg atgggcggtg tgcttggttc
    40021 cgtcactatt attattggtc tgggcgctat gctggggcgt atgatcgaac actctggtgg
    40081 tgcagagtca ctggctaatt atttcagtcg caagttaggt gacaaacgaa ctatcgctgc
    40141 gctgactctg gcagcgttct tcctcggtat tcccgtcttc tttgatgtcg gctttattat
    40201 tcttgcgcca atcatttacg gttttgccaa ggttgccaaa atatcaccac tcaaatttgg
    40261 cctgcctgtc gctgggatca tgctcactgt tcacgtggcg gtaccgccgc atccaggccc
    40321 tgtcgccgca gcggggttac tccacgcaga catcggctgg ctaaccatca tcggtattgc
    40381 gatttctatt cccgtaggga ttgttggcta ctttgcagcg aaaataatca ataagcgcca
    40441 atatgcgatg tcggtcgagg tactggaaca gatgcaactg gctccggcca gtgaggaagg
    40501 cgcgacaaaa ttaagcgata aaataaatcc accgggcgtc gcgctggtca cctcgctaat
    40561 tgttattcct atcgcgatta tcatggcggg tacggtttct gcaacactga tgccgccttc
    40621 gcatcccctg cttggtacgc tacagttgat cggctcacca atggtagctc taatgattgc
    40681 gctggtgctg gcattctggt tattggcttt acgtcgcggc tggagcttac aacacaccag
    40741 cgacattatg ggctctgccc ttcccactgc ggcggtagtg attttggtta ctggtgctgg
    40801 aggggtattt ggcaaagtgc tggtggaatc gggcgtcggt aaagcccttg ccaacatgtt
    40861 acaaatgatt gacctgcctc tgttaccagc cgcatttatt atttcactgg cgctgcgtgc
    40921 atcgcagggg tcagcaaccg tagcaatcct gacaaccggc gggttactct cagaagcggt
    40981 gatgggatta aatccgattc agtgcgtatt ggtgacgctg gcagcctgct ttggtgggct
    41041 tggcgcgtca catattaatg actcagggtt ctggattgtg accaaatatc tggggttgtc
    41101 ggtagcagac ggtctgaaaa cctggacagt gttaacgacc attctcggtt ttaccggatt
    41161 cttaattacc tggtgcgtat ggctcgtcat ctaataatcc agataagata tgatgtatcg
    41221 tttaagtgcc tgataagggt gtaatgtatg ccaatattaa cctcggtaat atctcgctac
    41281 cgaggtcaat tttcactttg caatataatt acagtctctt gcaagttgat tttatcttag
    41341 taataagaac cgaccaatct tattgccatt tcgatattag caggcataga tgcgtcgtag
    41401 ccatacatat ggccatattt cggacgtaat acttcagtta ctttcttaat aattgcttcg
    41461 cgctgggctc gcgcaaaatc agcatgatta tttgccgttt tattcataaa tttaccaaaa
    41521 ctttcttctt ttctgacttt aattgtcgta tcccgaagat cattaagaaa ctggcgttga
    41581 aatttaaaat catcgtgtga accaatatta ccatgcccgc caataaaata tttccagtca
    41641 agcatttcaa cctgacgcaa attactgtca tggtaaacca acgtatccga tacggcgaag
    41701 cccatcatcg gcagttggtc aggattcagg agatccgggc tgtgagctac tttttcctgt
    41761 tttagcaacc atacagaatg gtcgtcggta tgtcctgctt taacaaatcg gtgtaattca
    41821 atggtctgct tttcaaattt aaactggtca tcttttttac ttaaaacctg agtggcgacg
    41881 ggtaagcgac tgttcataaa ttgcattttt tcagcagtgg ctttactggc aataatacgc
    41941 agattcacgc cttctttctt cgcctcatcg ttccaaaatg gactatcgcc aatgtgatcc
    42001 acatggtaat gagaataaac cagcgctgtt acgggtaatg gagtcacatc acgaattgcc
    42061 tgtaataaat atttaccgcg accctccgga gcatcaaaca gcaaaacccc tttgtctccg
    42121 acataaaacg tagtggaata aaaatagcgt tgaacataat aagttctctc gccaattttt
    42181 tgtaaaatat aaggctgaga catatttctt tcaaaaaggt tattgagttt atcgcttggc
    42241 gatattattt tttccgctga aggttcaaca taacttgcat taactgtaga cgaatttgca
    42301 gtcgaaattt ccgtttgagc aaaaacacta ccagatataa gagaagaggc tagaattgta
    42361 tatattttaa tgccttgcat acgtagtcct ttattaatgt gttgttgatt tatagcatct
    42421 ctgaacagac tgttatttta gatattacta cgttacgaga ttcatttttc ttcgaat
//
LOCUS       DADTFG010000020        42075 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.020, whole genome shotgun sequence.
ACCESSION   DADTFG010000020 DADTFG010000000
VERSION     DADTFG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.020"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(13..1872)
                     /gene="gss"
                     /locus_tag="J1138_001058"
     CDS             complement(13..1872)
                     /gene="gss"
                     /locus_tag="J1138_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBA8452892.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2077..2984
                     /locus_tag="J1138_001059"
                     /pseudo
     CDS             2077..2984
                     /locus_tag="J1138_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide oxidoreductase"
     gene            complement(3117..3365)
                     /gene="hybG"
                     /locus_tag="J1138_001060"
     CDS             complement(3117..3365)
                     /gene="hybG"
                     /locus_tag="J1138_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBA8452893.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3378..3719)
                     /gene="hypA"
                     /locus_tag="J1138_001061"
     CDS             complement(3378..3719)
                     /gene="hypA"
                     /locus_tag="J1138_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBA8452894.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3712..4200)
                     /gene="hybE"
                     /locus_tag="J1138_001062"
     CDS             complement(3712..4200)
                     /gene="hybE"
                     /locus_tag="J1138_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBA8452895.1"
                     /translation="MTEEIAGFQTSPKAQVQVAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4193..4687)
                     /locus_tag="J1138_001063"
     CDS             complement(4193..4687)
                     /locus_tag="J1138_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBA8452896.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4687..6390)
                     /gene="hybC"
                     /locus_tag="J1138_001064"
     CDS             complement(4687..6390)
                     /gene="hybC"
                     /locus_tag="J1138_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBA8452897.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6387..7565)
                     /gene="hybB"
                     /locus_tag="J1138_001065"
     CDS             complement(6387..7565)
                     /gene="hybB"
                     /locus_tag="J1138_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBA8452898.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7555..8541)
                     /gene="hybA"
                     /locus_tag="J1138_001066"
     CDS             complement(7555..8541)
                     /gene="hybA"
                     /locus_tag="J1138_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBA8452899.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8544..9662)
                     /gene="hybO"
                     /locus_tag="J1138_001067"
     CDS             complement(8544..9662)
                     /gene="hybO"
                     /locus_tag="J1138_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBA8452900.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPRLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9851..10138)
                     /locus_tag="J1138_001068"
     CDS             complement(9851..10138)
                     /locus_tag="J1138_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBA8452901.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10257..11144)
                     /locus_tag="J1138_001069"
     CDS             complement(10257..11144)
                     /locus_tag="J1138_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBA8452902.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNFAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11301..12341
                     /gene="gpr"
                     /locus_tag="J1138_001070"
     CDS             11301..12341
                     /gene="gpr"
                     /locus_tag="J1138_001070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBA8452903.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEDNVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12381..12875)
                     /locus_tag="J1138_001071"
     CDS             complement(12381..12875)
                     /locus_tag="J1138_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBA8452904.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13066..13950
                     /gene="yghA"
                     /locus_tag="J1138_001072"
     CDS             13066..13950
                     /gene="yghA"
                     /locus_tag="J1138_001072"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBA8452905.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13989..14414)
                     /gene="exbD"
                     /locus_tag="J1138_001073"
     CDS             complement(13989..14414)
                     /gene="exbD"
                     /locus_tag="J1138_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBA8452906.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMIAALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14421..15155)
                     /locus_tag="J1138_001074"
     CDS             complement(14421..15155)
                     /locus_tag="J1138_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HBA8452907.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15407..16594
                     /gene="metC"
                     /locus_tag="J1138_001075"
     CDS             15407..16594
                     /gene="metC"
                     /locus_tag="J1138_001075"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBA8452908.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQEAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKTLDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGYEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16734..17393
                     /locus_tag="J1138_001076"
     CDS             16734..17393
                     /locus_tag="J1138_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA8452909.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17433..18332)
                     /locus_tag="J1138_001077"
     CDS             complement(17433..18332)
                     /locus_tag="J1138_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA8452910.1"
                     /translation="MKREEICRLLADKVNNLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18526..19689
                     /gene="yqhD"
                     /locus_tag="J1138_001078"
     CDS             18526..19689
                     /gene="yqhD"
                     /locus_tag="J1138_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBA8452911.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19794..20621
                     /gene="dkgA"
                     /locus_tag="J1138_001079"
     CDS             19794..20621
                     /gene="dkgA"
                     /locus_tag="J1138_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBA8452912.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGNALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20821..21747
                     /locus_tag="J1138_001080"
     CDS             20821..21747
                     /locus_tag="J1138_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBA8452913.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAIVRFRIFQDDKEKTSQTLKMVAESGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21798..22055
                     /gene="yqhH"
                     /locus_tag="J1138_001081"
     CDS             21798..22055
                     /gene="yqhH"
                     /locus_tag="J1138_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBA8452914.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKINQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22097..24316)
                     /locus_tag="J1138_001082"
     CDS             complement(22097..24316)
                     /locus_tag="J1138_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBA8452915.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKYTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24568..25317
                     /locus_tag="J1138_001083"
     CDS             24568..25317
                     /locus_tag="J1138_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HBA8452916.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            25640..27112
                     /locus_tag="J1138_001084"
     CDS             25640..27112
                     /locus_tag="J1138_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA8452917.1"
                     /translation="MSGNESIDAAHYDQVTTWPRGGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTHWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVTGGIH
                     TPVDGIAAAIQKLTEPQVKIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQS
                     AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVHGRPQWDKA
                     GAMFVSNVQPYEEMKLRMLNGGHSFLAYNGSLAGYEFIWQCMEDADFRSITRQLMINE
                     QARTLNPDLNIDAQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIEAVEKSQSVADPMNEQFQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKSIKATISQLLSDEQK"
     gene            27109..28125
                     /locus_tag="J1138_001085"
     CDS             27109..28125
                     /locus_tag="J1138_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA8452918.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINAFNPTQEDFIA
                     ALREAFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFNFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            28136..29146
                     /locus_tag="J1138_001086"
     CDS             28136..29146
                     /locus_tag="J1138_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HBA8452919.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            29219..30202
                     /locus_tag="J1138_001087"
     CDS             29219..30202
                     /locus_tag="J1138_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBA8452920.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFRLPFLFRDRDHYYRVLQSDLGKKILNSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYADM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            30244..30725
                     /locus_tag="J1138_001088"
                     /pseudo
     CDS             30244..30725
                     /locus_tag="J1138_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
     gene            30736..32040
                     /locus_tag="J1138_001089"
     CDS             30736..32040
                     /locus_tag="J1138_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBA8452921.1"
                     /translation="MEFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(32111..33523)
                     /gene="ftsP"
                     /locus_tag="J1138_001090"
     CDS             complement(32111..33523)
                     /gene="ftsP"
                     /locus_tag="J1138_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBA8452922.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(33598..34335)
                     /gene="plsC"
                     /locus_tag="J1138_001091"
                     /gene_synonym="parF"
     CDS             complement(33598..34335)
                     /gene="plsC"
                     /locus_tag="J1138_001091"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBA8452923.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(34570..36828)
                     /gene="parC"
                     /locus_tag="J1138_001092"
     CDS             complement(34570..36828)
                     /gene="parC"
                     /locus_tag="J1138_001092"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBA8452924.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(36966..38573)
                     /locus_tag="J1138_001093"
     CDS             complement(36966..38573)
                     /locus_tag="J1138_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA8452925.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDDGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(38682..39164)
                     /locus_tag="J1138_001094"
     CDS             complement(38682..39164)
                     /locus_tag="J1138_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8452926.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(39217..39609)
                     /gene="ygiW"
                     /locus_tag="J1138_001095"
     CDS             complement(39217..39609)
                     /gene="ygiW"
                     /locus_tag="J1138_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBA8452927.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            39761..40420
                     /gene="qseB"
                     /locus_tag="J1138_001096"
     CDS             39761..40420
                     /gene="qseB"
                     /locus_tag="J1138_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBA8452928.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            40417..41766
                     /gene="qseC"
                     /locus_tag="J1138_001097"
     CDS             40417..41766
                     /gene="qseC"
                     /locus_tag="J1138_001097"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBA8452929.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLKANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(41812..42042)
                     /locus_tag="J1138_001098"
     CDS             complement(41812..42042)
                     /locus_tag="J1138_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBA8452930.1"
                     /translation="MLESGDNICSVPSVSGEDRILQAMIAAFFLLTPLIILILRKLFM
                     REMFEFWLYVFSLVICLGCGWWLFWGRFIFCY"
BASE COUNT        10558 a        10898 c        10407 g        10212 t
ORIGIN      
        1 ctacaacatg tattactctt tcaccacaat taacggttca atatcactct cttttttgat
       61 gaccagtgat tcatcaccgc gcaaacacgt cccaccgtag ttgccgccaa cggtgaaagt
      121 acatacctga atgtatttac cgtccacttt cggcaaacac cacagttgct gatagatgtt
      181 tttctgctcg gcaaatttac cgctggtttt gtccagcacc tcttcatgat ggctgacgag
      241 gtcgatgtta ctgccacagc gaccggcgat cggtttaacg gcataaccgg ttttcaccag
      301 ctcatcattc acggtgaagt cagtatccaa cagataacga tggtgcggga acagagacca
      361 gaggatcggc agaatcgctt tgttaccggg gatcaccgtc catagcggct caaaaaccaa
      421 cacttccggg cgtaacagaa cgtctatcag acgaacttca ttttgtggat gcccggtacg
      481 gatcggcact gcggcaaatt cacggtcgct cacttcgcgg atttgatcaa atgccgtttc
      541 ccacgcccag gttttccata cgcagttaac cagtcgccct tccccatcaa tcagttgccc
      601 ggcagcatcc cagcccagtt catccaaccc acgcaagata cgcgtttcaa agcccgcctg
      661 gtgcagcgcc tgctccataa actgcgcgtg atagttttcc tcgatatctt tgtcctgcat
      721 gatatggaca aacggacgtg cacgactgtg tttccacgcg ccagccagtt cgttaatcag
      781 tccttccgca ggattgaagc cgttgccttt atagccctgc tccgcccaac gttcgaggat
      841 caagcccgct tcggtatgac aggaggcaga atcggcgttg tattcgtaga ctttcaggcc
      901 acgttcatcc atgcagaaat ccatacgacc agtgatcata tgatgacggc gacgctgcca
      961 ggagagacgc aaacgtggcc agaggatttt cggaatgtca aacagcgcca gcaggttgtc
     1021 atctttcagc accttgtcgg tggcgtgaag atacatcagg tgcagctcgt tggtggcttt
     1081 aatcagctcc tgctcggcac tctcggtaat ggtgtagtaa tgatacggat cctgattgat
     1141 cacctggccg ttggcctgta cataggcgtt ttgcagcgga tctttttcat ccagccattt
     1201 accgtcaaac tggcctttgt tttccagacg cgccccgctg attttcagca gttcgcctgc
     1261 aatttccggc tgtggcaagc tgtattcggt atcttccgtc tggatcatcc agcccagaat
     1321 agtggtgtca tcgaaggtgt ctttcagggt atagccgccg ttttcgacca ccatctccag
     1381 ctcgcgtgtc cactgttgcc cttgcggcaa cggggtatga atcacgttct gttccgcaat
     1441 acggactttg ttgccatgca attgggtaat gatggcgaca tggccagtgt ctttaaattc
     1501 accgccttta tcccagataa gaagcgcccc cgctaccggt gcacgcggcg aaccgttagg
     1561 aaatgcctgc aatggcagga tgttgtcatt gaccacttca cgcaggaagc gcagtgagaa
     1621 aatctcccat gccataccca cgtcagtgaa gactacaccg tagttcagaa agagaaaacg
     1681 gcgagcaaat tcaacgcatt gccatttgtg gcccatatat tcatcgtcga tatagctacg
     1741 gaatacggcg tcatcttcgt attcctgcgg atcgagagaa ctgtaatctg aagagtagat
     1801 tgctacccca cctggggcgt agcccaataa tgtcccgaac ggggcatcct ggctggtcgt
     1861 tcctttgctc atcactttac ctttaacaat acaacctaag cagttggcgt aattgttgtg
     1921 ctctgattac ctgctcagcg acattaaccg gacagaggtc agactaatca tacacctcat
     1981 cgcagcggct ggcgcaaagg gttaaaaaat tcacattctg tacagcaagt gacctgctac
     2041 actgcttcaa cactaccact cagaaggcaa ctcactatga cagacaatac ttatcagccc
     2101 gcgaaagtct ggacgtggga taaatccgct ggcggcgcgt tcgccagtat caatcgcccg
     2161 gtttctggtc cgacgcatga aaaaacgctg ccggtgggca aacatccatt gcaactttat
     2221 tcgctgggaa cgccgaacgg tcagaaagta acgattatgc ttgaggagct gctggcgctg
     2281 ggcgttactg gtgcagagta tgacgcctgg ctgatacgta ttggcgatgg cgatcaattc
     2341 tccagcggct ttgtcgaagt gaacccaaat tcgaagatcc cggcattgcg cgatcatacc
     2401 cataatccgc cgatccgcgt gtttgagtct ggatcgattc tgctttatct ggcagagaaa
     2461 tttggctact tcttgccgca ggatctggcg aaacgtactg aagcaatgag ttcttgccgc
     2521 aggatctggc gaaacgtact gaagcaatga gctggctgtt ctggttacag ggcgcggcac
     2581 cgttcctcgg cggtggtttt ggtcactttt accattacgc accggtgaaa attgagtacg
     2641 ccatcaaccg ctttactatg gaagccaagc gccttctcga cgtgctggat aagcaactgg
     2701 cgcagcataa gtttgttgcg ggcgatgagt acaccattgc ggatatggcg atttggccat
     2761 ggtttggcaa tgtggtgtta ggcggcgtat atgatgccgc agagtttctt gatgcgggca
     2821 gttataagca tgtacaacgc tgggcgaaag aagtgggcga acgtccggca gtgaagcgtg
     2881 ggcgtattgt taaccgcatc aacggaccgc tgaacgagca gttgcatgaa cgccatgacg
     2941 ccagtgattt cgagacgaat acggaagata agcgtcaggg gtaatggtta gcgttcgtcg
     3001 cagcaagcca tccagaccaa tgtaggccgg ataaggcgtt cacgccgcat ccggcaatcg
     3061 agcacaatgc ctgatgcgac gctgccgcgt cttatcaggc ctacaaaagc taatcatcac
     3121 gcgctggtaa tgtcgtaatc catttggcgc agtgcgtcta atgtggcttt ggcttcgtct
     3181 tcatcgataa tgctcatggc aaatccgacg tgtaccagta cccactggcc cagcagatcg
     3241 gcagggttac cttcacaaat cagggcgata ttcacgtcgc gcttgatacc acatacttca
     3301 acctgcgcaa gctggtgaat atcttcaccg acagccagca cctggcctgg aacgccaata
     3361 cacattgtta actccggtta ttcaacttca atacttttga cgattagcga atcgccggta
     3421 tcgacacgca accgctcgcc atgacagatc ggacactgcg catcgtgctg atgaatctcc
     3481 accacctggc tgcaatccca acaccaggcc tgggcgggtt tatagacgat atgtaaatcg
     3541 cacccttgcg ctaccgttcc gtggcagaca atttcaaaac taaaacggac ggcgctctcc
     3601 tcaacgcagg agagtgcacc aatttccagc cacacggcgg tgacgcgctt aacatcgtgc
     3661 tgctccgcct gccgttggat aatttcaacg gcgctctggc aaagcgacaa ctcatgcatt
     3721 ttcgccactc ctgcggccaa atagcagggc acgacgccct gcgtgtggta catccggatt
     3781 ggtgactggc agcgaaagga tcattcgggc gcagtcatcc gtcagccgtt ggccctcttc
     3841 aatcgacatg ctgtgcgaaa gcggcgacat cagcgagcag gagaggtatt gcgaaacacc
     3901 gtccagttca ccaacagtaa aggtcatagt gccatacggc agttgcagac caattttttc
     3961 actcactttg cgcagcggcc agagttgatc cgggccgggg aagataactg cactcagcat
     4021 ccacggggtg atcacacacc ccgtccactg accttcgaac agcgtaaagt cagaaacata
     4081 caccggcatt gaaggatgca gaaaagagag atcatgcatc gaacgccggg caatttcttc
     4141 aaaagctacc tgtacttgcg ccttcgggga ggtctggaaa cctgctatct cttcagtcat
     4201 gagtcgcctc ccgtgggata gcttccacgc ctgattcgcg cagcgcagcc agaacctgct
     4261 caagcgcagg ttcaatcatc gcttcgaccg tcggtgtcaa accgatgtgt ggctccagtg
     4321 attccgggat cacgccgacc agggtcagct ttttcggaaa ctcgccggtg aagcgcaggg
     4381 ccgacaatac gtcggccagg ccaagctgat gcggagagat tttgttggta aacaacgccg
     4441 gaacttcttc atcccgcagg atcatcatcg ttcccggtgc gttctttttt gacacaatgg
     4501 catccgcaat aatcagatga tcgcgatttg ccatgtcacc aagcagctcc attcccgccg
     4561 tgccgccatc gaggatctca acataatccg gcagaatgta tcgttgctct aacgcttcaa
     4621 caatccgcac accgatggct tcatcggtca gcaaaatatt gccgacccct aagactaaaa
     4681 tacgcattac agaaccttca ctgaaaccac ttcgttgccg tcggcatcca ctacgtgtac
     4741 cgcacaggcc atgcacgggt cgaaggagtg aatggtacgc accacttcca gcggtttatt
     4801 cggatcggca accggtgtac ccaccagcga ctgctcgtaa ggaccgacgt catcattgaa
     4861 gttacgcggg ccagagttcc aggttgatgg aacaaccgcc tggtagttac tgataatacc
     4921 gtctttgatc accatccagt gagagagcat accgcgcggt gcttcgagga agcctacgcc
     4981 tttgaactca cccgttgccg gaatgttcgg cttcacaaag gtggtgtgat cgcctttgcc
     5041 gatattggtg atcagtgcac tgtattgatt ttgcaggata tcctgcaatt cacagcagtg
     5101 aacggtacga ccaataatac ggcccagcgt ggagtgcagc tgcgccactt ccagcgtgtt
     5161 gccagtcagt ttctgataaa tcgcaacgat ttcattcagt ttgttctggg tagactcgcg
     5221 tcctgccgcc agtttcacca gcatattagc cagcggaccc acttctaccg ttttgccgta
     5281 gaaagtcggt gatttcaccc aggaatattt accgtcgtca gaccaaccat cataagccgg
     5341 aatggtggta ccttcccacg gtgcctgcgg cgcttcgtct ttataccagg agtgcttcgc
     5401 gctttcctga atccctttga tcaggtattc atcggaatga gaagtgatcg gacgatacga
     5461 ggacagatcc gcattctcaa tgtagccgcc cgggaacagg aagctgccat ttttactgtc
     5521 ggtcgggaat tccggcacgc tcaggtagtt caccgcacct ttaccgcgcg tcagccattc
     5581 cgggtagaac gcagcgataa ctgcagtatc aaccttgtaa acctgctcaa caaagtcgct
     5641 cagcttgtcg atgaaggact tgatgtacat caggcgctca aggttcagca cacccagacc
     5701 gtcgaggttg atcgggttcg cgacaccacc taccgccagg ttctgaatat gcggcgtttt
     5761 accgcccagc agcgccacaa cgcggttagc gtcacgctgg cactccagcg cctgcaggta
     5821 gtgcgctacc gcaatcaggt tcacttccgg cggcagtttc attgccgggt gaccccagta
     5881 gccattggcg aaaataccca actgaccgct ggcaaccaga tctttgatct tgttctgaac
     5941 tttggtgaac tcttccgggc tgttcagatg ccaggtcgaa acgcctttca gcatttcgga
     6001 ggctttggct ggatcagctt gcagtgcaga agtgatgtcc acccagtcca gcgccgaaag
     6061 ctgatagaaa tgaacaatat ggtcatgcgt ggtgtgcgca gccagaatga tgttacggat
     6121 gtattgcgcg ttaaccggaa cgtcgatatt cagcgcactt tctgccgcac gaacggaaga
     6181 cagcgcgtga gtggtagtac atacgccaca gatacgttgc acaatcatcc atgcatcgcg
     6241 cggatcgcgg tttttcacga tctcttccat gccgcgccac atggtaccgg aagcccatgc
     6301 tttcgaaacg acgccatttt cgatttcgca atcgatgcgt aaatgccctt caatacgggt
     6361 taccggatca atagtaattc tctggctcat gctttgctcg cctcatgacg attatgatcg
     6421 ttttgtttta aaggaggaag tatcggcagt agacgaatga gtacgatata agcgcaaatc
     6481 tcaatagcca caaaaccaat agaaatcaac agttcttccc aggtcgggaa gtaggcgtaa
     6541 ccgccacccg ggttgaatgc caccagcgaa taggtcagac gccaggttgc acaacctaac
     6601 agtgcactca gtgctgacag gaacagcatg cgggaatcat tacgcaactt cgccacacgc
     6661 agaacgacca gcgggaagag catcagcagg acttcaatcc agaacatcac ggagtagaag
     6721 tcaccggcaa acgctaacga cagcttgtcg cgatagataa gctcgccaaa gcgcagcacg
     6781 atgaaaatcg ccagcaacac actgatggtg ttggtcagct taacgaacag actcttttca
     6841 tccggaccgt tgccacgcag acccgcctgc accaacgaac cttcaaagat gacaatcgag
     6901 aagcccatga tgaacgccgt cagcagcgag aacagcggca acatttcata gctttgccac
     6961 agcggatgca ccttgtagcc cgccgagatc atcagcgacc ccattgaaga ctggtgcata
     7021 gttggcagca gcgcaccaag cgcgatgatg aagaacatta ccttgttcag acgtttgagt
     7081 gacaccttcc agcccagacg ttcaaacaat gccggagcaa actccagtgc catcacaccg
     7141 atgtagatgg tcatacagac cgccgtctcg aacagtaccg agttcacgtt gaagtgaccc
     7201 ggaatgtaga agtacggcag gttccagtag cgacccacgt cgatagtgat cgacaagcca
     7261 cccagtgaat aaccaaacag actcgccaac agcgccggac gcaccagcgg atggtattgc
     7321 ccacggttaa agacgtatac tgcccacgcc agcgcccagc caccacaggc aaagccggta
     7381 ccgatcagca ggtcaaacgc gatccacacg ccccacggga agccgccgtt caggtcggag
     7441 acagagccca gaccgaacac cagacgcttc acaatcagga gcatacagat gacgattaac
     7501 ggtccaaaaa tcatgaccgg tttactgatg attttgccgc ccagcggttg tggatcatga
     7561 ctcatgatcg tctcctccgt cgtgatggtc gtttttagtg ttgcgacgaa ccagcacggt
     7621 taagcccgcc agcacagcca gtggtagcat catgccttta tacagggtgt gttggacatg
     7681 ttcggaacgc gcaccagtag aaagatcgtc cagtttcggc aggtcgagat tttcataagg
     7741 cacacccgtc agtaccagaa cctgagtacc gccgccctct ttctcgccgt acagatgcgg
     7801 ataatatttc ggcaccgtat gcaggtaagt gtcgccagat ttcagcgtct gacgcggata
     7861 gtggtattcg ctgcccggct tcagcgccag acgttttttc gcctccgcca tcagctcttc
     7921 acgcgtacca aaaatcaccg cgcccgccgg gcacacttct acacagccag gcagaccgcc
     7981 tttatcgaga cgttccacac ctttctggtt gcacaactcg cacttatgca gcgcaccaaa
     8041 cgggttgttg tagtcgtact tcggcacgtt gtatgggcag gcgaccatgc agtaacggca
     8101 accggtgcac acatctttgt cgtaatggac aataccggtt ttcggatctt ttttcagtgc
     8161 agagaccgga cacacagaga cacagttcgg atcgacgcag tgcatacact gtttcttaat
     8221 gtacgcgtag ccattctcct cctggtcttt gttgacccct gtgccgctgg tccacacctg
     8281 aatgatgtta ttggtatacg gcgacagttt gtcgttgttc gaccaggtct gttccccttg
     8341 cgggttacgt tcagggaagt tgatatcctg acacttggtg acgcaagcct gacagcctac
     8401 gcacaaggtc gagtcgtaca acatccccag cgatcccgga attggcgggc ggttttcagc
     8461 agccgcatga ctgacagacg gcagcgcgcc cgtcagcaat gccccgcagg aggctgcttt
     8521 aataaaatta cgtctgttca cggttattct ccccgtgagt cagcgttatc tttcttttgc
     8581 tgacgaccca gttcacgcac cgccatcacg ctgacaccgg caaccagccc aaccacaccg
     8641 ccgagcaaac caatagcgcc tgccgagacg ttgccgcctt ctttagcgtt aacgtccggt
     8701 ttctgtgaac gcggagtctg gttttcgacg ttggcaagct gatggatgcc tttatggaag
     8761 ccgatacctt cttcgttaca gccatagcac gggtgaccaa tcgctaccgg ccacacgccg
     8821 ccaacatcgc agaattgcag cgttgagcag ttgccgtaag tttctggccc tttacagcca
     8881 aggtggtaca ggcaccagcc ttcgcggtgg ccttcatcac cgaactcttt ggcaaaacga
     8941 ccagcatcga agtgcgggcg acgttcacag tgttcgtgaa tcagacgacc ataggcgaaa
     9001 gtcggacggt ttttgtcatc cagtctcggc ggtttgccat aagtgatgat gtgcgcaacg
     9061 gtcgccagga agttgtgcgg gttcggcggg cagccaggaa tattgataac ggttttgccc
     9121 ggtagaactt cttgcaggct gactgcgcca gttgggttaa ctccagctgc ggcaacaccg
     9181 ccccacgcag agcaggaacc gatagcgata atggctgctg cgccttccgc cgctttgcgg
     9241 atgtgatcca caatcggctc accggcaacc atgcaataaa taccgttatc ttttaatggg
     9301 atggaaccat ccaccactaa cacatactgc cctttgtact tctcgagagc gttatgtttg
     9361 ttctcttcga cctgatgacc gaaggcggcg gaaagcactt cgtgatactc cagagagata
     9421 gtctccagca cgaggttttc taccgttgga tgcgttgcac gaagcagaga ttccgtacaa
     9481 ccggtgcact cctgcgcgcc aatccagata actggcggac gctgcgggtt agtaaccgat
     9541 tcggccatct ctgcagcggc tttgctactt aaccccatgg tggcggctaa tgctgcacaa
     9601 agcttcatga aatcacgacg gttaatgccg tgagaatgga tgagggtgtt atctccagtc
     9661 atttatagtt attccgttgc gaagacctgg catatatttt gcctcaatcg caaaatcaat
     9721 aatgcgatcg atgcgccatt taccacacat ttattatggt tatcgatatc atgatactgc
     9781 ggcggggcac taaaacgaag ggaagaagca ttagtgtaat taattaaagc aagataatac
     9841 gtatgtttga tcaattttct aggcgataac cggccataaa aaagcgcaac gtagaacagg
     9901 aattattctc acgaaagaaa tccattacca tctctttatc cagtccataa cggcgcgtca
     9961 gaatacccgc ttcccaggcg ctaagctggc gatctccggt cttttcgtac atccggtgtg
    10021 agtagcctaa tacaaaaccg cgtttataat cggcacagaa ttttgtcaca ttaaccgcac
    10081 tgtcggcatg cgttgctttt aatcccgcca ttaagccttt accaaaatgg ttattcatcg
    10141 ttcacctcat tcgctatata ttgtcatata tagcgatatt ttaggcaaag aacgacattt
    10201 gcgcacgcta ttttttcatg cttgtttaag acgtaatcct gtaagagata aacccatcag
    10261 gagagatatt tatcgaacca tttcagcgtc ctttgccagg caagatcggc ggcagatttg
    10321 tcataacggg gcgtggaatc attatggaat ccgtgattaa cccccggata tatatacgcc
    10381 tcatagacct tattattggc tttcaacgcc gcctcgtaag cgggccagcc ctcgttgatt
    10441 cggctatcca gttcagcgta gtggagcagt aaaggcgctt caatcttcgc cacatcggca
    10501 gtgggtgcct gacgcccata aaacggcacc gcgcaggcca gttccggata cgcgactgct
    10561 gccgcgttcg atacgccccc gccatagcaa aatccgctaa tacccacttt gcctgtcgct
    10621 tgcggatagc gttgcataaa ctcaattgcg gcaaagaaat cattcatcag tttggttgga
    10681 tcaacctgct gttgcagctc acgaccttta tcatcatttc ccggataacc tccaacggaa
    10741 cttaagccgt caggtgccag ggcgatatac cccgccttcg ccactcgccg cgtcacatct
    10801 tcgatatacg gattcagccc tcgattctca tgcaccacta ccacggctgg cgttttgccg
    10861 ctcatttttg cgggcttcac cagataaccc cgtacctcgc cgtgaccatt tggcgaggga
    10921 tacgtgatgt actctgcaac aatttccggg tcggtaaact ctacctgagt cgccagcgca
    10981 taatttggct tgagcaaatc aaacagcgct aatgccgtca tcccgcccac tgcatacttc
    11041 gccgcaaagt tgaggaactc acgtttcgag attttcccgt gagcgtaata gtcgtagtaa
    11101 tccagcaact cttgtgggaa atctttggcg gttaaacgcg gcatcgttgc actcctcaac
    11161 tggtgttttt ttaagcaaag cataagcgcg tgtttttgcc cagtttttcg tcactctgtg
    11221 agccagacta cgggatacgc gctggcgaat cgctaaacta gaaacattgt ttcgaaattg
    11281 aacgatggaa aggagaggtc atggtctggt tagcgaatcc cgaacgttac gggcagatgc
    11341 agtaccgcta ttgcggaaaa agtggtttac gcctgcccgc gttatcgctc ggtttatggc
    11401 acaatttcgg tcacgttaac gcgctggaat cacagcgtgc gatcctgcgt aaagcgtttg
    11461 atttaggcat tacgcacttt gatttagcca acaattacgg gccgcctcca ggaagcgcag
    11521 aagagaactt tggtcgcctg ctgcgcgagg attttgccgc ttatcgcgat gaactgatta
    11581 tctctaccaa agccggatac gatatgtggc ccggcccata tggctctggc ggctcacgta
    11641 aatacctgct cgccagcctc gaccaaagcc tgaagcgtat ggggcttgag tatgtcgata
    11701 tcttttactc tcatcgcgtc gatgaaaata cgccgatgga agaaaccgcc tctgcgctgg
    11761 ctcatgctgt acaaagcggt aaggcgctgt atgtcgggat ctcctcttac tcgccagagc
    11821 ggacgcaaaa aatggtcgag ttgctgcgcg agtggaaaat tccgctgtta attcatcaac
    11881 cttcgtacaa tttactgaac cgctgggtgg ataaaagcgg cctgctggat accctgcaaa
    11941 ataacggcgt gggctgcatt gcctttactc cactggctca gggattgctg accggaaaat
    12001 atctcaacgg tattccgcaa gattcacgga tgcatcgtga agggaataaa gttcgtggtc
    12061 tgacgccgaa aatgctcacc gaagccaacc tcaacagcct gcgcttattg catgaaatgg
    12121 cacagcagcg tggacaatca atggcgcaaa tggcgttaag ctggttgctg aaagatgagc
    12181 gagtgacgtc ggtattgatt ggtgccagcc gcgcggagca acttgaggat aacgtgcagg
    12241 cgctgaataa tctgacattt agcaccgagg agctggcgca gattgatcag catatcgccg
    12301 atggagagct gaatctgtgg caggcgtctt ccgataaatg acctgttaat aacgggcgcg
    12361 agaaccgcgc ccgaatcaga tcagtgatta tgacgagtca gtcggtcaag atagcccatc
    12421 acaaatgcag agagcacaaa cgtcagatgg ataatgacgt accacatcag tttgttatca
    12481 gggacatttt tcgcatccat aaagacgcgc agtaagtgaa tggaagaaat tgccacaatc
    12541 gacgctgcta ctttgttttt cagcgacgtt gcgtccattt tccccagcca gttcagcttc
    12601 tctttgttct cagagatatc gagttgtgag acgaaattct cgtaaccgga aaacatcacc
    12661 atcaccagca aaccgccaac cagcgtcata tccaccagcg acagcaacac gaggatcaaa
    12721 tctgattccg ccatcgagaa gatattcggc agtacgtgaa taatctcctg gaagaacttc
    12781 agcgccaggg caactaacgc cagcgaaagg ccaaagtaca cgggggcaag cagccagcga
    12841 gaagcataca ttgcattttc aagaaaacgt tccatacatt cctgtcgtgt agttaaaacg
    12901 ggcaacagta tatcgcaatt aagtaaacag cacgcaacag caaatcaaca tatacgttaa
    12961 acctttgtcg cataattctg agggttaggg atcttcctgg ttcccttatc aacgcatcga
    13021 gagcgtctac ccttaaagga cgttgaacct gaagggagaa aaatgatgtc tcatttaaaa
    13081 gacccgacca cgcagtatta cactggtgaa tatcccaaac agaaacaacc gacgccaggc
    13141 atccaggcga agatgacacc ggtaccggat tgcggcgaga aaacctatgt tggtagcggt
    13201 cgcctgaaag atcgtaaagc actggtgaca gggggcgatt ccggaatcgg tcgcgctgcc
    13261 gccattgctt acgcgcgtga aggggctgac gtggcgatca gttatcttcc cgtggaagaa
    13321 gaagacgctc aggatgtgaa aaagatcatt gaagaatgcg gacgcaaagc cgttctgctg
    13381 ccaggcgatt taagcgatga gaaattcgca cgttcgctgg ttcacgaagc acacaaggcg
    13441 ttaggcgggc tggatattat ggcgctggta gccgggaaac aggttgccat tcctgatatt
    13501 gcagacctca ccagcgaaca gtttcaaaag acctttgcca ttaacgtttt cgcgttgttc
    13561 tggctaaccc aggaagcgat ccccctgcta ccgaaaggtg caagtatcat caccacttcc
    13621 tcaatccagg cataccagcc aagcccgcat ttactggact atgcggctac gaaggcggca
    13681 attctgaact acagccgtgg cctggcaaaa caggtcgcgg agaaaggtat tcgggtgaat
    13741 attgtcgcgc caggcccgat ctggacagca ctgcaaattt ctggcggaca aacgcaggat
    13801 aagatcccgc agtttggtca gaaaacgccg atgaagcgtg cggggcaacc ggcggagctg
    13861 gcccctgtat atgtttacct ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac
    13921 ggtgtctgcg gcggcgagca tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg
    13981 catcattttt atttcgcttt cgcagtttct tcgccgacca gacctatttt caggtaaccc
    14041 gcctgatgca gcgtatccat taccttcatc aacgtctcgt aatcgacagt tttatccgca
    14101 cggaagaaga tggtggtgtc tttcttgcct tcggttaacg cattcaacgc cgcaatcatt
    14161 gtttcatcgg tgaccggatc gttaccaata aacatcgagt tgtctgcttt cactgacaga
    14221 taaaccggtt tttccgggcg cggctgcggc gtgctggtag aagcaggcaa attcaccttc
    14281 acatctaccg tcgctaacgg tgccgccacc ataaagataa tcagcaaaac caacatcacg
    14341 tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag gttttcgtta
    14401 agatgcatcg ccatcggaca ttatcctgcg cgtaattttt gtgcgacacg aaccggatgt
    14461 gcagcggcgc tggcttccag atccaggtca cggctttgca acaacaatac ctgcgctgca
    14521 acatcaccca gcatcgcttt aaagccgcca atctggcgtg caaatacgtt atagataacg
    14581 accgcaggaa tcgctgccac gaggccgatt gccgttgcta acagagcttc tgcaatacct
    14641 ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga taccaataaa gctgttcatg
    14701 atgccccaga ccgtaccaaa cagaccaacg aacggcgaaa tcgcgccgat ggttgccaga
    14761 tagccgttac cacgccccat ttgacgacct actgcggcga cccgacgctc cagacggaag
    14821 ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc cagctcattc
    14881 tgcgcttcat tgagcaaatg caggctcaag cttttgctac caaaatcagc ggcgatatcg
    14941 ttggcctggt ttaaggaacg cgcttcagcc agcaattgct gctcgcgctt aagacgacgc
    15001 ttctgattga agaactccac gctcttactg aagaagattg cccaggtgac tacggaggcc
    15061 aaaataagcc caatcatcac gcacttaacg acaatatcgg cgtgctgata cataccccag
    15121 acggagagat ccgtctgcat taaattatta cccactgtgt atctccagga cgcaagtcac
    15181 aaaatctgtg cataataata tcaaaacgac gtcgaattga tagtcgttct cattactatt
    15241 tgcatactgc cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa aagtcaccag
    15301 cacgccattt gcgaaaattt tctgctttat gccaattctt caggatgcgc ccgcgaatat
    15361 tcatgctagt ttagacatcc agacgtataa aaacaggaat cccgacatgg cggacaaaaa
    15421 gcttgatact caactggtga atgcaggacg cagcaaaaaa tacacgctcg gtgcggtaaa
    15481 tagcgtgatt cagcgcgcgt cttcgctggt ctttgacagt gtagaagcca aaaaacacgc
    15541 gacacgtaat cgcgccaatg gcgagttgtt ctatggacgg cgtggaacgt taacccattt
    15601 ctccttacaa gaagcgatgt gcgaactgga aggtggcgca ggttgcgcgc tgtttccttg
    15661 cggggcggca gcggttgcca attccattct tgcttttgtc gaacagggcg atcatgtgtt
    15721 gatgaccaac accgcctatg aaccgagtca ggatttctgt agcaaaatcc tcagcaaact
    15781 gggcgtaacg acatcgtggt ttgatccgct gattggtgcc gatatcgtta agcatctgca
    15841 gccaaacact aaaatcgtgt ttctggaatc gccaggttcc atcaccatgg aagtccacga
    15901 tgttccggcg attgttgccg ccgtacgcag tgtggtcccg gatgccatca ttatgatcga
    15961 caacacctgg gcagccggtg tgctgtttaa gacactggat tttggcatcg atgtttctat
    16021 tcaagccgcc accaaatatc tggttgggca ttcagatgcg atgattggca ctgcggtgtg
    16081 caatgcccgt tgctgggagc agctgcgaga gaatgcctat ctgatgggcc agatggtcga
    16141 tgccgatacc gcctatataa ccagccgtgg cctgcgcaca ttaggtgtgc gtttgcgcca
    16201 acatcatgaa agcagtctga aagtggctga atggctggca gaacatcccc aagtagcgcg
    16261 agttaaccac cctgctctgc ctggcagtaa aggatacgaa ttctggaaac gagactttac
    16321 aggcagcagc gggctatttt cctttgtgct taagaaaaaa ctcaatgatg aagagctggc
    16381 gaactatctg gataacttca gtttattcag catggcctac tcgtggggcg ggtatgaatc
    16441 gttgatcctg gcaaatcaac cagaacatat cgccgccatt cgcccacaag gcgaaatcga
    16501 ttttagcggg accttgattc gcctgcatat tggtctggaa gatgtcgacg atctgattgc
    16561 cgatctggac gccggttttg cgcgaattgt ataacattgc cacttttgga caattttgca
    16621 gacattttat tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt cccggacgat
    16681 tcaggagtac aataggcaga taaaggctta aacgctgttc cacaggaaag tccatggctg
    16741 ttattcaaga tatcatcgct gcgctctggc aacacgactt tgccgcgctg gcggaccctc
    16801 atattgttag cgttgtttac tttgttatgt ttgccacgct gtttttagaa aacggcttgc
    16861 tgcccgcctc atttttgcca ggcgacagct tgttgatact ggctggcgca ttgattgccc
    16921 agggggttat ggattttctg cctacgattg cgattctgac cgccgcagca agtctgggct
    16981 gctggctaag ttatattcag gggcgctggt tagggaatac caaaacggtg aaaggctggc
    17041 tggcacagct tcctgctaaa tatcaccagc gcgccacctg catgtttgac cgccacggtc
    17101 tgctggcgct gctggctgga cgttttcttg catttgtccg tacgctgctg ccaaccatgg
    17161 cgggaatttc cggtctgcca aaccgccgct tccagttttt caactggtta agcggattgc
    17221 tgtgggtcag cgtggtaacc agtttcggct atgccttaag tatgattccg ttcgttaaac
    17281 gccatgaaga tcaggtaatg acgttcctga tgatcctgcc aattgccttg ttaaccgctg
    17341 gcttgttggg tacgctgttt gtggtgatta aaaaaaaata ctgtaacgcc tgacgatttt
    17401 ccccgttccc ggttgctgta ccgggaacgt atttaattcc cctgcatcgc ccgcattctt
    17461 gccgcatctt ctcccggcgt cacaccgaag taacgtttaa actcacggct aaattgcgat
    17521 gcgctttcat agccgacgcg catcgccgcc gcgctggcct tcatgccgtc gtggatgatc
    17581 atcatccgcg ccttatgcag acggtaattc ttcaaatact gcaacggcga ggtgctggtg
    17641 acagacttaa aattatggtg gaacgccgat acgctcatgt tggcttctgc cgccagttgc
    17701 tcgacgctca ggttttcggt gtatttattc tcaatccgtt tcagcacgcg actaatcagg
    17761 ctgaagtgag tctggcggct gaccagtgcc agtaacgcgc cgccgcaagg tccggtcagc
    17821 acgtagtaca gaatttcgcg gataatctgt ttgccgagaa tgcgcgcatc cagcggtcgc
    17881 tccatcacat ccagtaaccg ctctgccgcg cataaaatct cttctgataa cgtggcggag
    17941 ttaataccgc tggctgccat tgacggctga aaatgttcat cttcgccaat gtccatcaac
    18001 agttcctgta actgcaaaat atcgacattg agacgcaacc ctgccagcgg cacctctgac
    18061 gtggcatagg tttcgcactc aaacggcaac ggcaccgtca gcaggagata ttcattagcg
    18121 tcataacgaa acacgcgttc attgatataa ccgattttat gtccggaaaa gagaattatg
    18181 atgccaggct cgtacatcac cggtgtgcgt gcgaaaggcg tctcgccata caacaaacgc
    18241 acatcgggca acagctctga caaactattt tctttatttt tcaggttatt aactttatcc
    18301 gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta atttcttagg aataatgcgg
    18361 caatttgatt gtgcgcaatt ttgtagcatt tctccagcac tctggagaaa taggcaagac
    18421 attggcagaa atgagcattg agaggtacgg cgctggcgac cacaatgaaa aacatcaggc
    18481 agaccgatct ctgccctcat attgacccag caaagggagc aagtaatgaa caactttaat
    18541 ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa
    18601 attcctcacg atgctcgcgt attgattacc tacggcggcg gcagcgtgaa aaaaaccggc
    18661 gttctcgatc aagttctgga tgccctgaaa ggcatggacg tactggaatt tggcggtatt
    18721 gagccaaacc cggcttatga aacgctgatg aacgccgtga aactggttcg cgaacagaaa
    18781 gtgacgttcc tgctggcggt tggcggcggt tctgtactgg acggcaccaa atttatcgcc
    18841 gcagcggcta actatccgga aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa
    18901 gagattaaaa gcgccatccc gatgggctgt gtgctgacgc tgccagcaac cggttcagaa
    18961 tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg acaagcaggc gttccattct
    19021 gcccatgttc agcccgtatt tgccgtgctc gatccggttt atacctacac cctgccgccg
    19081 cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca ccgtggaaca gtatgttacc
    19141 aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag gcattttgct gacgctgatc
    19201 gaagatggtc cgaaagccct gaaagagcca gaaaattacg atgtgcgcgc caacgtcatg
    19261 tgggcggcga ctcaggcgct gaacggtttg atcggcgctg gcgtaccgca ggactgggca
    19321 acgcatatgc tgggccacga actgactgcg atgcacggtc tggatcacgc gcaaacactg
    19381 gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata ccaagcgcgc taagctgctg
    19441 caatatgctg aacgcgtctg gaatatcact gaaggttcag acgatgagcg tattgacgcc
    19501 gcgattgccg caacccgcaa tttctttgag caattaggcg taccgaccca cctctccgac
    19561 tacggtctgg acggcagctc catcccggct ttgctgaaaa aactggaaga gcacggcatg
    19621 acccaactgg gcgaaaatca tgacattacg ctggatgtca gccgccgtat atacgaagcc
    19681 gcccgctaag ctttttacgc ctcaaacttt cgttttcggg catttcgtcc agacttaagt
    19741 tcacaacacc tcaccggagc ctgctccggt gagttcatat aaaggaggaa cgtatggcta
    19801 atccaaccgt tattaagcta caggatggca atgtcatgcc ccagctggga ctgggcgtct
    19861 ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc
    19921 gctcgattga taccgccgcg gcctacaaga acgaagaagg tgtcggcaat gccctgaaaa
    19981 atgcctcagt caacagagaa gaactgttca tcaccactaa gctgtggaac gacgaccaca
    20041 agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc
    20101 tctacttaat gcactggccc gttcccgcta tcgaccatta tgtcgaagca tggaaaggca
    20161 tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc
    20221 atcacctgca acgcctgatt gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac
    20281 ttcacccgct gatgcaacaa cgccagctac atgcctggaa cgcgacacac aaaatccaga
    20341 ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt tttcgatcag aaagtcattc
    20401 gcgatctggc agataaatac ggcaaaacac ctgcgcagat tgttatccgc tggcatctgg
    20461 atagcggcct ggtggtgatc ccaaaatcgg tcactccttc acgcattgtc gaaaactttg
    20521 atgtctggga tttccgtctc gacaaagacg aacttggcga aattgcaaaa ctcgatcagg
    20581 gcaagcgtct cggtcccgat cctgaccagt tcggcggcta acgtgcaaat tatcccggtg
    20641 gcggtaatgt tccgctaccg gacttttcag aaatcattta ttcccctcgc gtcccgcccg
    20701 ttgctactct tccttgttca ggaatgccaa atataaggac atcatcatgc agagccggaa
    20761 gctcttaaaa gaacaaccca tctatatccg ggataaatgc aacggagagg tgaaaaacag
    20821 atgaaaataa tacttctgtt tttagcagcc ctggcaagtt ttaccgtaca cgcacagccc
    20881 ccctcacaga ccgtagaaca aacagtccgg catatttatc agaactataa atcagatgcc
    20941 actgcccctt attttggtga aaccggagag cgggcgataa catctgcgcg tattcaacag
    21001 gcgcttaccc tgaacgacaa tcttacgctg ccgggcaata ttggctggct ggattatgat
    21061 ccggtttgtg attgtcagga ttttggcgat ctggtgttag aaagcgtagc gataacccaa
    21121 actgacgccg atcatgccga tgccattgtg cgctttcgta tctttcaaga cgataaagaa
    21181 aagacctcgc agacactgaa aatggtggcg gaaagtggtc gttgggtcat tgacgatatt
    21241 gtcagcaatc atggcagcgt cttacaagcg gttaatagcg agaatgaaaa aacgctggcc
    21301 gctatagctt cgttgcaaaa agaacagccg gaagcctttg ttgccgaact ctttgaacat
    21361 attgctgatt atagctggcc gtggacgtgg gtggtttccg actcttaccg ccaggcggtc
    21421 aatgccttct ataaaaccac cttcaagacg gccaacaatc ccgatgaaga tatgcaaata
    21481 gaacggcaat ttatttacga caatccgatc tgttttggcg aagagtcgtt attttcacgc
    21541 gttgatgaaa ttcgagtcct ggagaaaacc accgattccg cccgcattca tgttcgtttt
    21601 acgctgacca atggcaacaa cgaagagcaa gaactgattt tacagcggcg cgaaggtaag
    21661 tgggaaatcg ctgattttat ccgcccgaac agcggcagcc tacttaagca gattgaggca
    21721 aaaaccgccg ccagattaaa acaatgagct gaattaaata acaattagcc ggaacaataa
    21781 ataaaaggga acactatatg aaaacgattt tcaccgtggg agctgttgtt ctggcaacct
    21841 gcttgctcag tggctgcgtc aatgagcaaa agatcaatca gctggcgagc aatgtgcaaa
    21901 cattaaatgc caaaatcgcc cggcttgagc aggatatgaa agcattacgc ccacaaatct
    21961 atgctgccaa atccgaagct aacagagcca atacgcgtct tgatgcacag gactatttcg
    22021 actgcctgcg ctgcttgcgt atgtacgcag aatgataaaa aaatccccgg cagcattctg
    22081 ttaccgggga tattttttaa cgtccaaccg ccgctttagg gcgtttcttc gcaccagcat
    22141 tctgcaggcg agattgcgta gacgacgctt tttttgccgt agcaggcgtc tgacgctggg
    22201 tcgccatcgg cgtatattta gtcaacgccg gacgggtatt gcggttctga cggcgagctt
    22261 cacgcatctc ttcaatggtt ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat
    22321 gctttttgcc catcgcttcc agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt
    22381 aacgcaacaa cgctttatgc aggcgacgct gcttgtcgcc cttcggtacg aagacgtctt
    22441 cactcttata accaatcttc gccagcgggt ttttgccggt gtaatacatg gtggtcgagt
    22501 tagccagcgg cgacggatag aagttctgca cctggtcgag gcggaagcga tgctttttca
    22561 gccatagcgc cagattcacc atatcttcat cacgcgtacc cgggtgcgcg gagatgaaat
    22621 acgggatcag atactgttct ttacctgcct gtttcgagta agtatcgaac agctctttaa
    22681 agcggtcata gctgcccatg cccggcttca tcatcttcga taacggccct tcttcggtat
    22741 gttccggggc aatcttcaga taaccgccaa cgtgatgggt cgccagctct ttgatatagc
    22801 gcggatcttc tacggcgatg tcataacgca caccagaggc aatcaggatc tttttaatgc
    22861 ctttcagatc gcgcgcacgg cgatagaggt taatcgtcgg ttcgtggtta gtgtccatat
    22921 gcggacaaat atccggataa acgcacgaca aacgacggca ggtttgctca gcgcgtggcg
    22981 atttgcaacg caacatatac atgttggcag taggcccacc gagatcggaa atcacgcccg
    23041 taaaacctgg aacagtgtcg cggatcgctt cgatctcatt aataatcgaa tcttcggaac
    23101 ggctctgaat aatgcgccct tcgtgctcgg taatagaaca gaaagagcag ccgccaaagc
    23161 agccacgcat aatgttgacc gagaaacgaa tcatttcgta agccggaatt cgggcattac
    23221 cgtaagccgg atgcggcacg cgcttgtacg gcagcgcaaa gacgctatcc atctcttcgg
    23281 tagaaagcgg aatagcaggc gggttgatcc atacatagcg gtcgccatgt ttttgcatca
    23341 atgcgcgggc gcagcctggg ttagtttcgt ggtgcagaat gcgcgaagca tgggcgtaca
    23401 gcactttatc gcccttcact ttctcgaaag aaggcagcaa cacgtaggtt ttttcccacg
    23461 gtttcgggcg tggtggctgc acggttacag ctttggcttc ctgctttttc ggagctaccg
    23521 gtttgttatc cgcgcacggc aaatcttcgc catacggatg cgggattggg tcgattttcc
    23581 ctggggtatc aagacgggtg gaatccacac cgctccagcc aggcaacgcc tcttttacga
    23641 taatcgcggt attacgcaca tcgcggattt cactaatcgg ctcgcccatc gccagacggt
    23701 gcgccacttc caccagcgga cgctcgccgt taccgaacat cagcatgtca gctttggaat
    23761 ccaccaacac cgaacggcgc acggtatccg accagtaatc ataatgcgcg gtacggcgca
    23821 ggctggcctc aataccgccg aggatcaccg gcacatcttt ccacgcctct ttacaacgct
    23881 gggtataaac cagtgtggcg cgatccggac gcttacccgc gacgttatcc ggtgtgtagg
    23941 catcgtcatg acgtaaacgg cgatcggcgg tatagcggtt gatcatcgag tccatgttgc
    24001 cagcagtaac accgaaaaac agattcggtt tacccagacg cataaagtcg tctttactgc
    24061 tccagtctgg ctgggcgatg atcccgacgc gaaagccctg cgcttccagc atacgaccgc
    24121 aaatcgccat cccgaagctt gggtgatcga catacgcgtc gccagtaacc aaaatgatgt
    24181 cgcagctatc ccagcctagt tgatccatct cttcacgtga catcggcaaa aacggtgccg
    24241 gtccaaaaca ggcggcccag tactgcggcc aggagaacag gtcgcgatcc ggttggatca
    24301 gggagataga gctcattttg cttccagaaa tggtaaaaaa ataatcaaag gccggggatt
    24361 atacgctgta tcacgcgcgg aaatgaagtg atgttgatga acggtggcga aataccctgg
    24421 tgaggttttt cactgcccga cttgggcaag cacatttaag gctggcggta tcgtggcaac
    24481 tgccgctact atttatgcca ctcagcaacg ttcaaatcat attgcacgtc gatcggctat
    24541 ccgaggacac ccacagtaag gcaaggtatg gaacaggtta ttacaaagcg ccgctattac
    24601 gatattggtc tacagattga agagctgctg tattcaggcg tatttaaggc cggagaacgc
    24661 ttaccttcag aacgtgaact gagcgagcgg tttaatacca gccgaactac cattcgtgaa
    24721 gccatcatta tgctggaact gaaaggcgta ttgaatgtga agcaagggtc agggattttc
    24781 tttgtcgact ccaccgataa gctcaaccag aaatcattaa tgccctattc agaaattggc
    24841 ccgtttgaac tgttacaggc acgtcaggta attgagagca atattaccgg ttttgctgcc
    24901 agccagatca gctttaacga gttacaagag ttgaagaaaa tcattggatt acaggaaaaa
    24961 gccatcgctg cggaaagtga taaatttgaa gacctggatc accgatttca tagcattatt
    25021 gccgaagcga cgcaaaatcg ggtactcatc aaacaagctg ctgaattatg gcgggcagtt
    25081 cgtacagaaa acccccgctg gaagaaactt aactacaagt acctgcatga aaagcatttg
    25141 cgtctgcaat ggctggaaga tcaccgggct attttcctgg ctctgcaaca gaaagattca
    25201 gagctcgcca gggaagcgtc ctggagacat ctggaaaaca gcaaaaatga actgatcaaa
    25261 atcttcaaac aagacgcttc catcagcgat tttgatgact ttttcttcgc tcgttaacct
    25321 tcattagtcc agagctggcg gaaaaaataa cgccggctct gccttccgcc ctccactggt
    25381 acacgctctc tttttaattg gtctaataaa tcgactatca tcagatatct tagattagcg
    25441 gcaattatcc taaaaactga cctctaaaaa actttccttt cattttattc atataactca
    25501 ttgatacaga tataaataac gccttgttgt tctgtttcgt acttttacct ttctctgcaa
    25561 gcgtgatgca tctctctgtt tttggtctaa tgaattatgt aaattggtat acacacaagc
    25621 caccaggagt aagagcataa tgtcagggaa cgaaagtatc gatgccgccc actatgatca
    25681 ggtaaccacc tggcctcggg gcgggttaca ggcagatatt gtccatattg gttttggcgc
    25741 ttttcatcgc ggacaccagg ctgtctacac ggatctcact aatcagcttt ctgacaccca
    25801 ctgggggatc tttgagatca acctgtttgg tgatgctcaa ctgatcgaaa acttaaatgc
    25861 gcaaaatggg ctgttttcgg ttgtggaaac atctgcatcg caatctacct cacgccttgt
    25921 gcgttcggta actggcggta ttcatacccc cgtggatggc attgccgcag caatccagaa
    25981 attgactgaa cctcaggtaa aaatcgtttc attaaccatc accgagaaag gctattgtct
    26041 cgattcgcaa acgcgatcgc ttgatctcac caatggatta atcaaacacg atctacaaaa
    26101 cccggatgcg cctcagtcgg ctattggctt gatcgtctgt gctttgcaac aacgtaaagc
    26161 ggcagggctc gccgctttta gcgtgctctc ctgcgacaac ttgccagaca atgggcatct
    26221 gacgcgaaat gccgttctcg gttttgcccg acaactggac cagcccctgg ctcagtggat
    26281 cgaagaaaat gtctcatttc caggcacgat ggtcgatcgc attgttccgg caatgaccga
    26341 atcgcaattc gccttactgg aaactaaaac cggttatgcc gatccctgcg ggatcgtctg
    26401 cgaatcattt cgtcagtggg tgattgaaga taattttgtg catgggcgac cacaatggga
    26461 taaagcaggc gcgatgtttg tcagcaatgt tcagccttat gaagagatga agttacgcat
    26521 gttaaacggt ggccattcat ttctggccta taacggctca ctggctggct atgaatttat
    26581 ctggcaatgc atggaagacg ctgattttcg ttccattacc cgccaactga tgattaatga
    26641 acaagcccga acacttaatc cagacttaaa tatcgacgcc caggaatacg ccgacctgtt
    26701 aattgaacgc tttagcaacc gtaatgttgc acaccgtacc gggcaaatcg ctatggacgg
    26761 ttcacaaaag cttccccagc gtgcgctgac gccctggctg aaattgcatc agcaaaaaca
    26821 aaacaatgct gttctgtcac tgctcgttgc tggttggttg cattatgtca ttgaagctgt
    26881 tgagaaaagc cagtctgtcg cggatccaat gaatgaacaa tttcaggcgc taataaagga
    26941 acaacaagac gcatggcaac aggcgctcgc attactgcac cttagcgcca tatttggtga
    27001 tttaagcaac catcagccat ttataaatga aataaaaatc gcctttgcaa atataaaaaa
    27061 caaaagcatc aaggccacca tcagccaatt attatcggat gagcagaaat gaaaacatta
    27121 atttgtcagc agcctggcgt tatggaatat gtggaaaagg atattcccac accagcagat
    27181 aatgaagtgc tgttaaaaat aaaagctgtg ggtatttgtg gtactgatat tcacgctttt
    27241 gccggcagac agcctttttt tagctaccca cgtgtattag gtcatgaaat atgcgccgaa
    27301 gcggtttcgc gaggcagcca gtgccaaaca gcacaatcag gccagcgcta ttccgtcatc
    27361 ccatgcattc cgtgtggcga gtgcgcagcc tgtcgggaag agaaaacgaa ctgctgcgaa
    27421 cgtgtttcgc tgtatggcgt gcatcaggat gggggtttta gtgagtacct tgcggtacgt
    27481 gaagacaacc ttgtgcctct ccctgacgag gtcagcgaca gtgccggagc attggttgaa
    27541 tgtttcgcca ttggtgcaca tgccgttcgt cgggcagaga tcaaggctga acaaaacgta
    27601 ctggtgattg gcgctgggcc aatcggtctg gctaccgcag ccatcgcccg ggctaaaggg
    27661 gcacatgttg ttgttgctga tattgactgt caacgtcgcc agcacgttgt ggatcatctg
    27721 gcgattaatg ccttcaatcc cacacaggaa gattttattg ccgcgcttcg tgaggccttt
    27781 ggaggcgaac tggcttgcgt agtactggat gcgacgggaa ataaagcttc aatgagtcat
    27841 gatgtcaatc ttattcgtca tggcggcaaa attgttttca tcggtttgta cattggtgaa
    27901 cttgttattg acgatccgac cttccataaa aaagagacaa cgttactcag cagccgcaat
    27961 gccacacggg aagattttgc gttggtgatt gaactgatgc gcagcaataa aattcacgaa
    28021 aatttaatga aaaaccaggc gttcaatttc tttagtgttg gcgaagatta ccagcgtaac
    28081 gttgtagaaa ataaaaatat ggtcaagggt gtgatcactt tttaagggaa caaatatgac
    28141 aactgtttat gtcagtgaag aaaatttaaa atcactggtg caccacaaat tacataccgc
    28201 cgggctggat accgacacca cacaacaggt gacagatgtt ttagtacatg ccgatattac
    28261 cggtgtgcat tcacatggtg ttatgcgtgt cgaacattat tgcacccgcc ttgctgccgg
    28321 agggttaaat aaagccccgc agtttagcat tgagcaaatt tcaccgtcag tggccattct
    28381 cgactctgac gacggaatgg ggcattccgc attaattagc gccactgagc acgctattaa
    28441 actggcccag caagagggtc tcggttttgt tagcgttaaa aacacgtctc actgtggtgc
    28501 gttatcttat tttgcagaga tgatcaccaa caaagggttg gttgctatcg taatgacgca
    28561 aaccgatacc tgtgtggctc cccatggcgg cgccgagcgc tttttgggaa ctaaccccat
    28621 cgcctttggt ttcccggtgg aaaacagcca tccgatgatt gttgatatgg cgaccagtgc
    28681 cacggctttc ggcaaaatac ttcatgcaaa ggaaaccgga aaacatattg gtgaagggct
    28741 ggcgatagat aaaaacggtt atggcaccac tgatccgtat aagattgaaa acctgctacc
    28801 tttcggccaa cacaaaggtt caggcattgc actggctatt gatgcactga ctggcgtgct
    28861 gatgaatgcg aattttggca accatattgt tcgcatgtat ggtgattatg acaagatgcg
    28921 taagctggca agtttggtta ttgccatcga tccgaaaaag ctcggcaatc ctgtttttgc
    28981 aaaaactatg gcgaaaatgg tcactgagct gcatgccgtt aaaccggctc ctggtgtcga
    29041 aaaagtgtta gcgccgaacg atccgcaaat gcactacaaa gaaaaatgcc aacaggaagg
    29101 tattccggtt ccggcaggaa tattccatta tctggcagag aattaatcac tattttttat
    29161 tataacgata gaaaagcgct gtatttatcg tttctgttat attaaccagg agcaggatat
    29221 gaaagcactt cgtcctctga cggcatcgct tatgctgtta accagttgtt tattaatatc
    29281 taataccaca ctggcaaaaa caacattaaa actgagtcat aatcaggata aaagccatgc
    29341 tgtccataaa gcattaagct atttagcgga taaaaccaaa gagtattcta atggtgaatt
    29401 agttattcgc atttacccaa atgcgacatt aggtaatgag cgtgaatcac tggaattaat
    29461 gaactccggt gcattacaga tggttaaggt taatgccgca tcactagaat catttgctcc
    29521 ggattacagc cttttcaggc tgccgttctt attccgcgac cgtgatcact attatcgtgt
    29581 tctgcaaagt gatttaggta aaaaaatact taattcatca gaaagcaaag gttttgttgg
    29641 tattacgtac tatgacggcg gtgcgagaag tttttattcc aataaaccga ttacaaaacc
    29701 tgaagattta gcgggaatga aaatcagggt tcaacaaagc cccagcgcca ttgcaatgat
    29761 gaaagcactc ggtggtgtcg ctaccccgat ggcgcaaggc gaactctaca ccgcacttca
    29821 gcaaggagtg gttgatggcg gagagaacaa taccgttgtc tatgccgata tgcgtcacgc
    29881 cgaagtcgca aaagtctatt cacgtgatga acacaccatg gtacctgatg tgttaattat
    29941 cagcaccgac gtgttgaata aacttggtga taaagagcgc acggcattat taaaagcagc
    30001 cgatgagtca atgatgcaga tgaaggacgt catttggcct gcggctgaaa aagaagccta
    30061 cgacaaaatg aaagggatga acgcaacagt tgttgatgtt gataaatccg ctttcaaaga
    30121 acgcgtcaaa ccgttatacg atgaattcaa ggcgaaagat gcacaatcag ccaaaaacct
    30181 tgagctagta gaaagtatgt aaccctaatg ggcaggtaat tcctgccttt tcatggaaga
    30241 cttatgcgaa cactcactca tattcttaat aagatccttg ccggatgttg ctgcattatt
    30301 ctcgccatta tggtgtctgc gtaacctggc aagttattgc acgttttatt tttaacgctc
    30361 ccagcactgt gcttgatgag ttcacccaga ttttatttat gtggattatt ttgcttggcg
    30421 gtgtatatac tgcgggttta aaaaaacatc tggccattga tttgttggct caaaaacttc
    30481 cggctgcatc tgttctgacg cttgacagtt ttatccaaat tattattacg gtctttgcgg
    30541 ttattttcat gatctatggc gggaatattg ttgttgaaaa agcacaacat gttggacaaa
    30601 tctctccagt attaaaatgg ccaatggata aagtatattg ggttatgcct gtcagtgggc
    30661 ttattctggt ctggtattca gtaatgaata ttattgacaa ctatcgtaaa cggaattctc
    30721 attaacgagg gaaatatgga atttgaatat atctaccccg tcttaatttt atttggcagt
    30781 tttgccgtca tgctggcaat aggtgtgcca attacttttg cgattggtct ttcttcgctg
    30841 ttatctatta ttactgcctt gccacccgat gccgccattt ctgttatttc acaaaaaatg
    30901 actgtagggc tggatggctt tacgctatta gccattccct tcttcgtgtt agccggaaac
    30961 attatgaata ccggtggtat agccagacga ctggttaacc tggcgcaagc attagttggg
    31021 cgtcttcctg gctcactggc tcattgtaat atcctcgcga atacactgtt tggtgcgatt
    31081 tcaggttcag ccgttgcgtc agccgcagcg gtaggtggga ttatgtcacc actgcaagaa
    31141 aaagagggat atgatccggc gttttcagca gcggttaata ttgcctctgc ccccattggc
    31201 ctgatgatcc caccgagcaa tgtgttgatt gtttattccc tcgccagtgg cgggacttct
    31261 gtcgctgcgt tgttcttagc tggatatctg ccgggcatcc tcaccgctgt tgccttaatg
    31321 tttgtagctg cactttatgc acgacgtcac cattacccag tggccgaacg catcaattac
    31381 cgtcagtttt tacaggtatt ccgcgaatca attcctagcc tgatgcttat ttttatcatt
    31441 attggcggta ttatcgcagg ggtatttacg cccacagaag catcagcaat agcggtaatt
    31501 tatagtttag ccctggcgat gatttaccgg gaaatcactc ttaagaagct caatgatatt
    31561 ctgttagatt cggtagtaac cagttcaatt gttctgttac tggtaggctg ttcaatggga
    31621 atgtcatggg ccatgacgaa tgctgatgtt cctgagttga tcaacgaact gattacccgc
    31681 gtttcggata acaaatgggt cattctgttt attatcaata tcattctgtt gatcgtcggt
    31741 acctttatgg atatcacgcc ggcgatcctg atatttacac ctattttcct gccgatcgcc
    31801 cagcatctgg ggattgatcc gattcacttc ggcataatta tggttttcaa cttaaccatt
    31861 ggcctttgca caccacctgt cggcaccatt ctgtttgtgg ggtgcagcat tggtaaggtc
    31921 agtatcgaca gggcaataaa accattactg ccgatgtttc tggcattgtt tgtagtaatg
    31981 gcaattattt gttatttccc gcagcttagt ctgatgctgc caggattatt ttcgacctga
    32041 tacacaatga agactgcata ctgagtaatt ttcaatgccc caccactctg tggggcattg
    32101 aattggtcca ttacggtacc ggattgacca acagttgccc aatcgagcca cggtccgcca
    32161 tttccagcgt ctgactgttg aagtagaacg ggaagtgcgc ccaggaaggc tgaccgaaat
    32221 aaacaagcag ctccacttgt ccgtcaaccc aaacggtatc tttccagcct ctgtcttccg
    32281 ggaacggcat cgcgccattc acgttacgga tctggaacat cacgccttca atatggaacg
    32341 cttgcggctc gtccgcgcgc accgtccagc gttcccacgt tccttgctgc gcagtaacat
    32401 caatacggtt gacgtcccac aactgcccat taatccccgg gtcatcaccc agactaatat
    32461 cgcgactgcg aatcggcgaa ccggccatga tttcagttgg cagcaagcgc atcggaagac
    32521 tgtcagtgac cagcggcaga aggccggttg ggcgcagcgt tagcaccagc gtagaaacca
    32581 gaatgctgga tggctcaaag aagccacgaa tacgatcaac aatgctcgcc gcttcgccac
    32641 aggtaatcga cacttcatca ccgttgctca tatccaccag aatctcacga cgctcgcccg
    32701 gtgccagcga aagttgcttc accgatacag gagcgggcag gaatccctga tcgccagaaa
    32761 tcacatgtaa cgggcgacca tcgctcatct gtagttgata gcgacgagag ttcgacgcgt
    32821 tcaacagacg caagcgcacc cagccacgcg agacttcaac gtacgggctt tgtacaccgt
    32881 taaccagtag cgtatcgcca acaaagccgc cgcttcccgg ttcgttatat tctggcgtac
    32941 caaagttatc cagccgttta tcctgaataa tgacaggaaa atcatccaca ccataatggt
    33001 tggggatagg cagcgattta ctgacttcat cttccaccag ccacattccg gcaaggccgt
    33061 tataaacctg ctgagccgtg cggttgggag tattggcgtg ataccacaga gtagctgcgt
    33121 tctggcgaat gggcagtacg ggtgcccagt cagcatttgg cgacatcatc cgtgccggac
    33181 cgcccatcag cgggccaggt acctgtagcc cggccaccgt cattgagaca ttttctgtca
    33241 ggcggttgct gtaaataagc ttaacatcgt cgcccttcca gacgcggata gtcggcccca
    33301 ggtaacgacc attgattccc caaaccgacg cgcgtgtccc tggcgtaaat gaccagtgcg
    33361 cacgttgtac agtcataaac agcggttgcc cacggcgaga ttcaagcagc ggcggaacgg
    33421 gtagcggttg ctgttgcccg gctgcgctgg ccttcagggg aacagcgcct gcacaaagtg
    33481 caatccccga tgcctgaatg aactgacgcc gactgagtga catatttgct ccatgtaaaa
    33541 ctggctaaag taataccgtg caggaaagtg ttccccgcaa aaatacagat tgccgactta
    33601 aacttttccg gcggcttcgc gttctgcgac ttctttatcg agctcggcga ttttttgttc
    33661 cattatcgaa cgacaatggg cagccaactc gcgaacctga tctttgccat actgactgac
    33721 gtcaattggc ggcagcattt cgacaatcac cagaccgttg tgtagccgat taagattaat
    33781 cttattcgaa gttgtagaga cgcacacggg tataatcggg acgcccgccg caattgccgc
    33841 gtgaaatgcc ccggtcttga acggcagcag gccgcgacca cggctgcggg ttccttccgg
    33901 gaacatccag atggaaatac ggcgtttttt gaagtgattc actacttccg caatagtgcc
    33961 gtgagcttta gtgcgattgt ttctgtcgat caataagttg ccggttaacc agtacaactg
    34021 cccgaagaag gggatccaca gcaagctctt tttacctacc gtcactgtcg gcggctgcac
    34081 aatgttcgat gccgtcacca tgtcataatt gttctggtgg ttagcaatat agatagcatt
    34141 gccgtagctt tcagcgtctg caggtttgcg gcactccact ttcaggccga aaagcggcgc
    34201 aagacggcca aacatatgcc caaaggtggc cacatgtttc gggttacgcg ggctgaaaag
    34261 gcagtaaatg gagccgaata cacagactaa gatgctgtaa atcacggtaa taataagacg
    34321 aaagatatat agcataacac cctctgaagg tctgagagac tggcattgta cgtcacctga
    34381 aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt taatcgcaac
    34441 gcatgaataa acaacggttt caagggaatt tttatcgttt ccggggattg aatacgctgc
    34501 cggatgacga cttaacgttt catccggtgt tccttgcaag cgggaggaaa cagcgccctc
    34561 cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt cgatctcaac
    34621 acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt cgccagtgac
    34681 tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa tggtcagcgt
    34741 gctttgcggc ggcagaacgt acagttgcgc caggccatcc tcgccacgcg cggcttctgc
    34801 cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca gatcgcttac
    34861 cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg aagcatcttc
    34921 aatcaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag ccttacctgc
    34981 acggttacgc gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg catcggaagc
    35041 catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg ctccaggcgg
    35101 caacgttaat ttgccggtga gcggctcgcc ctgaccacgc gccgacggca gcgtaatcgg
    35161 gtcgatggca tagctacgac cggtggaatc aacaaacact accggttggt tgctcttacc
    35221 tttcaccgcc gctttgaagc tatcgcccgc tttataattc aggcccggcg cgtcgatatc
    35281 atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga caggttcaga
    35341 cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca acggcgaacg
    35401 acgttcgtca ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca ggttattcat
    35461 tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca gctcactctg
    35521 ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca gttcgaggat
    35581 cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt tcggttcatc
    35641 ttcattacga atgatctcaa tcacttcgtc gatattgaga aacgccacca gcaaaccttc
    35701 gaggatatgc aggcgcttga ggactttctc cagacgatag ttcagtcggc ggcgcacggt
    35761 atcgcggcgg aacaccagcc attcggagag gatttccagc aggtttttca ccgccggacg
    35821 accatccaga ccgatcatat tgaggttaat gcgatagctc ttttccagat cggtggtagc
    35881 gaagaggtgg ttcatcacct gatccatatc cacgcggttg gagcgcggca caatcaccag
    35941 acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg gcagcttttt
    36001 gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct gatgcggcaa
    36061 tgcgctgata accaccgcgc catcttcttt cttccacacc gcgcgcatac gcactgaacc
    36121 acgtccgttc tcgtagattt tacgaatctc ggcgcgcgaa gtgataattt ccgcttcagt
    36181 cggataatcc ggcccctgca cgatatccag cagctgatcg agagtggttt tcggctggtc
    36241 gattaatgcg attgccgcct gagccacttc acgcaggtta tgcggtggaa tatcggtcgc
    36301 catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac gggcaggcag
    36361 cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg tcccctgccc
    36421 cagctcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt aacgcattgc
    36481 cgcgaacgat ttcggatcgt ccggcgcacc ccagttcccc tgaccatcaa ccagcggata
    36541 acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcac tatcgccgtg
    36601 cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa atttggcgct
    36661 ggcattcagg cccagctcag acatcgcata cacaatgcgg cgctgaacag gtttcagacc
    36721 atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt ttaagtaggc
    36781 gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta attctgattc
    36841 ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac attatctgcc
    36901 gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg acggcgataa
    36961 aggcatcaat gtgccttgat atacaactct ttgctgtaga cataatcctg gggattatgc
    37021 agcggaaaac cgccaacgta gggtttaagc agtttgatta acggctgata gtagatggga
    37081 atcagcggtg cctgctggtt gatgatcact tctgcctgct gatacaacgc attacgcttt
    37141 gtcgcatcag tgatctgcgt ggcctggttt agtaaggcgt catactgcgc atttttccag
    37201 tgaccgacgt tttcttcact atcgcttttg agcgtgttca ggaagctgga ggcatcattg
    37261 tacgtcgcat cccacgactg ccgagacagc ataaaatcac cggctcgtcg ggcatcaaga
    37321 taggttttcc actccattgt gcgcagcgtc acctgtgcac ccagccattt tttccattcg
    37381 gaagacaacg ctatcgcggt cttttcatgc agatcgtact tgttgtagaa cagctcaaag
    37441 cgtagcggat gagaggcgtc gtatcccgcc tgtttcagca agacttttgc catcgcgacg
    37501 cgctcactca ttggcttttg cagttcatcg aacgtcgtcg cgctaaagcc ttttacctct
    37561 ggcggcgtca gcgtggttgc gggcgttctc aaccccagta ccttttgcgc aataagctgt
    37621 cgatcaaccg taagatatag cgcccgacgc acccgcacat cgttaaatgg cggtttctca
    37681 aggttaaagt tgtaatattc gctgttcaga cgcgggataa ttcgtagctc gccaggcagt
    37741 gatttttcaa tggcgggaat ttgctgcgcc ggaacccagg tgagatcgac ctctcccgca
    37801 cgatagcggt tatagccggt gaccgaatta tccagcgcca gatactcaac ctgttgcaat
    37861 actgtatgtt gcgcatcgcg gtactttgga tttttgcgtg cagtaatctt ttcgttaact
    37921 acccatttat caagcacaaa ggcaccgttg taaaccatgt tctctggctt actccagcta
    37981 tcgccatgtt tagcgatgac atgatgagga accgggaaca gcgtcggcca ggcgagcatc
    38041 gtcgtgaacc aaggaactgg ctgctcaagc gtaacttcaa gagtacgatc atccgtcgct
    38101 ttgacaccca gcgatgtaac atctgcttta cccgcaacaa tagccgcggc attgttaatg
    38161 tgcgcctgtg ccagatagcc agcaaaaggg cttgccgttt tcgggtcaac cgcgcgctgc
    38221 cagccgagga caaaatcctc tgccgtcaga ggctgaccgt ctgaccactg caaaccgcta
    38281 cgcagatgga aaatatagcg cttgccgtcg tcaagtatct cccagcgttc agcctgagcg
    38341 ggctgcacct ggccttcacc gtccatccat accagacctt caaacagatc cagcacaatc
    38401 tgcgcggcag tattctcctc taccttttgc gggtcgagcg tgcctgggtc gctatgattg
    38461 ttgtaacgaa agacttgttg cggggcgagc ggtgtgttgg cgggaacgtc agcagcgtag
    38521 agaggagccg cacttaccag gctgaccagc cataataaat ttcgcgtata catggtattc
    38581 cttaccggag aaattataag cccgacaaca atacgcctgt ggcattgttt gctcaaactt
    38641 atcgcgagtg atttggctca cactccggta aagattcccc atcacgccaa cggcacataa
    38701 atatccgttt ccagtcgccg ctctgtcatc ccttcggcaa gattggtgta gtgaaacagg
    38761 atcggggctt tgcgcatttt ttcgccgctt gcaggtagcc agtggcgaat gatgccccat
    38821 atcgtatgtg aaatatcgtc cagctcgcca acatggcggg caacagcata acgcccaccg
    38881 gtaagttcgc cattactcac gccataacga ttatcgggga tcggttcgct aacgctgccg
    38941 cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag ggtcgtccca ggcgacgcca
    39001 aaagtctggc tttggttaac aggagaaagc cccgtctctt tacgccacat gataaatttc
    39061 gccacgctgt agttgagcaa ctcagggcta cagcggtgcg gcaacatcgc cacagggatt
    39121 gatgggaaat cgataatatt tacatccagt gtcaggtttg tcattttgcc tccctgcgaa
    39181 attattcccg ggaacgcgtc ccgggagcgg taacaattac ggatttactt tgcggatctg
    39241 tttgacgtca atttcaacag agttccagtc tttatcgact tccccctgaa tctcaaccgt
    39301 atctttcggc gtcaccgtca cgccgttcca gcgtttgtgg tcgatatcaa cattgatagt
    39361 accgctggca tctttgaaca cgtagagatc gtcagagatg cgttcaacga tattgccgcg
    39421 tagggttacc caggtgtcgt cacgcaggga ttttgcgctt tctacggtcg ttacgctgcc
    39481 gttcggcccc tggaatcctc cggcctgact ttgcgttgcc gatgggccag aaaaacctcc
    39541 ctgctctgct gccataaccg gtgcactgca cagggccatt actgcgatta ctgctgcgaa
    39601 ttttttcatg tttattactc cctttaatgt ctgtttccga gcatttaaca agatagtcct
    39661 taacaacttc ttaagggaaa aaaataaaat ttaatgctgt acagagcgcg ttacaacacg
    39721 gtttactggc agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt tactgataga
    39781 agatgacatg ctgattggcg acggcatcaa aacgggcctt agtaaaatgg gttttagcgt
    39841 cgactggttt acacaaggtc gtcagggaaa agaagcgcta tatagcgcgc cttatgatgc
    39901 ggtgatcctg gatttaacct taccaggcat ggatggtcgc gatattttgc gcgaatggcg
    39961 agaaaaaggt cagcgtgagc ctgtattgat cctgactgcg cgcgatgcgc tggcggaacg
    40021 tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg cgttgataga
    40081 agtcgccgcc aggctggaag ctctgatgcg ccgaaccaac ggccaggcca gcaacgagct
    40141 gcgccacggc aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg ctggcgaacc
    40201 cttaacgctg aaaccaaaag aatttgccct gctggaatta ctgatgcgta acgctggtcg
    40261 ggtactgccg cgcaaactga ttgaagagaa actgtatacc tgggacgaag aggtcaccag
    40321 taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg attttattcg
    40381 taccgttcat ggtattggct acacattagg tgagaaatga aatttaccca acgtcttagt
    40441 ctgcgagtca ggctgacgct aatcttttta attctggcct cggtgacctg gctgctttcc
    40501 agctttgtcg cctggaaaca aacaacggat aacgtcgatg agttgttcga cacccaactg
    40561 atgctgtttg ccaaacggtt aagtacgctc gatctcaacg aaatcaacgc ggcggatcgc
    40621 atggcacaga cgccaaataa attaaaacac ggtcatgttg atgacgatgc gctgaccttt
    40681 gccatcttta cccacgacgg cagaatggtc cttaacgatg gcgataacgg agaagatatt
    40741 ccctatagct atcagcggga aggttttgct gacgggcaac tggtcggtga agacgatccc
    40801 tggcgttttg tctggatgac ctcacctgat ggcaaatatc gcatcgttgt gggtcaggaa
    40861 tgggaatacc gtgaagacat ggcgctggcg attgttgccg gacaattgat cccgtggctg
    40921 gtcgcactgc cgattatgtt aatcatcatg atggtactac tgggtcgtga actcgcgccg
    40981 ctgaacaaac tggcgctggc actacgtatg cgtgacccag actcggaaaa accactaaac
    41041 gcgacgggcg tacccagcga agtgcgccca ctggttgagt cgctaaatca actgttcgcc
    41101 cgcacacata cgatgatggt tcgtgaacga cgctttacct ccgacgcagc tcacgaactt
    41161 cgtagcccgt taacggcgct gaaagtgcaa accgaagttg ctcagctctc tgacgatgat
    41221 ccgcaggcgc ggaaaaaagc gctgctccaa ttacattccg ggatcgatcg cgctactcgt
    41281 ctggttgatc aactgctcac gctatcgcgg ctggactcac tggataacct tcaggacgtc
    41341 gcggagatcc cgcttgaaga tctcctgcaa tcgtcggtga tggatattta ccacacggcg
    41401 cagcaggcga aaattgacgt gcgactgaca ctcaaagcca acggcatcaa acgcaccgga
    41461 caaccgctat tgctaagttt gttggtgcga aatttgctgg ataacgccgt gcgttacagt
    41521 ccacaaggta gcgtggtaga cgtcacgctg aatgccgata atttcatcgt gagggataac
    41581 ggccccggtg tgacaccaga ggcattggcg cgaattggcg aacgcttcta tcgcccaccc
    41641 ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat cgccaagctg
    41701 catgacatga atgttgaatt tgggaatgcg gaacaaggtg gatttgaggc gaaggtaagc
    41761 tggtaacttc tctgtctatg agagccgtta aaacgactct catagacttt actaatagca
    41821 aaatataaac cgtccccaaa aaagccacca accacaaccc agacaaataa cgagcgagaa
    41881 tacatatagc caaaactcga acatttcccg cataaagagt tttcttaaga taagaataat
    41941 aagtggtgtc agaagaaaaa atgctgcaat cattgcttgc aatatacgat cttcgcctga
    42001 aacagaaggt acactgcaaa tattgtctcc cgactcaagc atccagtcat acttattgaa
    42061 agcaaaaaaa gaatc
//
LOCUS       DADTFG010000021        41361 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.021, whole genome shotgun sequence.
ACCESSION   DADTFG010000021 DADTFG010000000
VERSION     DADTFG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.021"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            249..875
                     /locus_tag="J1138_001099"
     CDS             249..875
                     /locus_tag="J1138_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBA8452931.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            896..2998
                     /locus_tag="J1138_001100"
     CDS             896..2998
                     /locus_tag="J1138_001100"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBA8452932.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFIYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            3011..3649
                     /locus_tag="J1138_001101"
     CDS             3011..3649
                     /locus_tag="J1138_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBA8452933.1"
                     /translation="MNHQDELPLAEVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADILQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3713..4381
                     /locus_tag="J1138_001102"
     CDS             3713..4381
                     /locus_tag="J1138_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA8452934.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4378..5163
                     /locus_tag="J1138_001103"
     CDS             4378..5163
                     /locus_tag="J1138_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBA8452935.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5167..5979
                     /locus_tag="J1138_001104"
     CDS             5167..5979
                     /locus_tag="J1138_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452936.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5985..7595)
                     /locus_tag="J1138_001105"
     CDS             complement(5985..7595)
                     /locus_tag="J1138_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBA8452937.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKRENHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFNQM"
     gene            complement(7721..8026)
                     /locus_tag="J1138_001106"
     CDS             complement(7721..8026)
                     /locus_tag="J1138_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBA8452938.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8135..8211)
                     /locus_tag="J1138_001107"
     tRNA            complement(8135..8211)
                     /locus_tag="J1138_001107"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8175..8177),aa:Val,seq:gac)
     gene            complement(8216..8292)
                     /locus_tag="J1138_001108"
     tRNA            complement(8216..8292)
                     /locus_tag="J1138_001108"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8256..8258),aa:Val,seq:gac)
     gene            8600..9856
                     /locus_tag="J1138_001109"
     CDS             8600..9856
                     /locus_tag="J1138_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8452939.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGIQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVVLLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATQVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNSLKLQPDQDGIGATLQPA"
     gene            complement(9897..11270)
                     /gene="mdtK"
                     /locus_tag="J1138_001110"
     CDS             complement(9897..11270)
                     /gene="mdtK"
                     /locus_tag="J1138_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBA8452940.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11485..12126
                     /gene="ribE"
                     /locus_tag="J1138_001111"
     CDS             11485..12126
                     /gene="ribE"
                     /locus_tag="J1138_001111"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBA8452941.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12166..13314)
                     /gene="cfa"
                     /locus_tag="J1138_001112"
     CDS             complement(12166..13314)
                     /gene="cfa"
                     /locus_tag="J1138_001112"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBA8452942.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQEHCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13605..14816)
                     /locus_tag="J1138_001113"
     CDS             complement(13605..14816)
                     /locus_tag="J1138_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBA8452943.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14929..15861
                     /locus_tag="J1138_001114"
     CDS             14929..15861
                     /locus_tag="J1138_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8452944.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYTF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15858..16883)
                     /gene="purR"
                     /locus_tag="J1138_001115"
     CDS             complement(15858..16883)
                     /gene="purR"
                     /locus_tag="J1138_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBA8452945.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17182..17271
                     /gene="ynhF"
                     /locus_tag="J1138_001116"
     CDS             17182..17271
                     /gene="ynhF"
                     /locus_tag="J1138_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBA8452946.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17436..18605
                     /locus_tag="J1138_001117"
     CDS             17436..18605
                     /locus_tag="J1138_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8452947.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLVIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSVRKQPETVCVANS"
     gene            complement(18751..19332)
                     /gene="sodB"
                     /locus_tag="J1138_001118"
     CDS             complement(18751..19332)
                     /gene="sodB"
                     /locus_tag="J1138_001118"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBA8452948.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19460..20287)
                     /locus_tag="J1138_001119"
     CDS             complement(19460..20287)
                     /locus_tag="J1138_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HBA8452949.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNATSGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20622..20969
                     /gene="grxD"
                     /locus_tag="J1138_001120"
     CDS             20622..20969
                     /gene="grxD"
                     /locus_tag="J1138_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBA8452950.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21341..21988)
                     /gene="rnt"
                     /locus_tag="J1138_001121"
     CDS             complement(21341..21988)
                     /gene="rnt"
                     /locus_tag="J1138_001121"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBA8452951.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(22091..22498)
                     /gene="gloA"
                     /locus_tag="J1138_001122"
     CDS             complement(22091..22498)
                     /gene="gloA"
                     /locus_tag="J1138_001122"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBA8452952.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(22579..23676)
                     /gene="nemA"
                     /locus_tag="J1138_001123"
     CDS             complement(22579..23676)
                     /gene="nemA"
                     /locus_tag="J1138_001123"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBA8452953.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(23713..24312)
                     /locus_tag="J1138_001124"
     CDS             complement(23713..24312)
                     /locus_tag="J1138_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA8452954.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            24415..24654
                     /locus_tag="J1138_001125"
     CDS             24415..24654
                     /locus_tag="J1138_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBA8452955.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKSAPSDEPEQPSLF"
     gene            24703..25599
                     /locus_tag="J1138_001126"
     CDS             24703..25599
                     /locus_tag="J1138_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBA8452956.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            25680..26201
                     /gene="sodC"
                     /locus_tag="J1138_001127"
     CDS             25680..26201
                     /gene="sodC"
                     /locus_tag="J1138_001127"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBA8452957.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQLIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26202..28214)
                     /locus_tag="J1138_001128"
     CDS             complement(26202..28214)
                     /locus_tag="J1138_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA8452958.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTIARIISPLRPTNVADYRHIAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(28214..29071)
                     /locus_tag="J1138_001129"
     CDS             complement(28214..29071)
                     /locus_tag="J1138_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA8452959.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(29074..29310)
                     /locus_tag="J1138_001130"
     CDS             complement(29074..29310)
                     /locus_tag="J1138_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBA8452960.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            29511..29945
                     /gene="slyA"
                     /locus_tag="J1138_001131"
     CDS             29511..29945
                     /gene="slyA"
                     /locus_tag="J1138_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBA8452961.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(29992..30459)
                     /gene="slyB"
                     /locus_tag="J1138_001132"
     CDS             complement(29992..30459)
                     /gene="slyB"
                     /locus_tag="J1138_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBA8452962.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            30733..31842
                     /gene="anmK"
                     /locus_tag="J1138_001133"
     CDS             30733..31842
                     /gene="anmK"
                     /locus_tag="J1138_001133"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBA8452963.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            31940..32269
                     /gene="mliC"
                     /locus_tag="J1138_001134"
     CDS             31940..32269
                     /gene="mliC"
                     /locus_tag="J1138_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBA8452964.1"
                     /translation="MSMKKLLLICLPVLLTGCSSFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            32328..32984
                     /gene="pdxH"
                     /locus_tag="J1138_001135"
     CDS             32328..32984
                     /gene="pdxH"
                     /locus_tag="J1138_001135"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBA8452965.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            33113..34387
                     /gene="tyrS"
                     /locus_tag="J1138_001136"
     CDS             33113..34387
                     /gene="tyrS"
                     /locus_tag="J1138_001136"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBA8452966.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFMKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEDGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            34449..35309
                     /gene="pdxY"
                     /locus_tag="J1138_001137"
     CDS             34449..35309
                     /gene="pdxY"
                     /locus_tag="J1138_001137"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HBA8452967.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVKNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(35353..35958)
                     /gene="gstA"
                     /locus_tag="J1138_001138"
     CDS             complement(35353..35958)
                     /gene="gstA"
                     /locus_tag="J1138_001138"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBA8452968.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(36064..37566)
                     /gene="dtpA"
                     /locus_tag="J1138_001139"
     CDS             complement(36064..37566)
                     /gene="dtpA"
                     /locus_tag="J1138_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBA8452969.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(38177..38812)
                     /gene="nth"
                     /locus_tag="J1138_001140"
     CDS             complement(38177..38812)
                     /gene="nth"
                     /locus_tag="J1138_001140"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBA8452970.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(38812..39507)
                     /locus_tag="J1138_001141"
     CDS             complement(38812..39507)
                     /locus_tag="J1138_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBA8452971.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAV
                     AERALPNGETGNV"
     gene            complement(39511..40131)
                     /gene="rsxG"
                     /locus_tag="J1138_001142"
     CDS             complement(39511..40131)
                     /gene="rsxG"
                     /locus_tag="J1138_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBA8452972.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(40135..41193)
                     /gene="rsxD"
                     /locus_tag="J1138_001143"
     CDS             complement(40135..41193)
                     /gene="rsxD"
                     /locus_tag="J1138_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBA8452973.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHVPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWMIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT        10590 a         9985 c        10678 g        10108 t
ORIGIN      
        1 gttcacttat aatttcttgc tttgtgattt taattgataa cgatcaatgt taagagccca
       61 gaataatcac attatttttt ctcctaacca taaaggatta gtttatcagc gttaaatttt
      121 ttctcataca ctatcgggtt agggaaaata tctggtgttg aggacagagt cagcagcgta
      181 ttagttgagt gcctaatatc aggctgctga attgtcagca ctgtgtttgc gaagaggatg
      241 cgaaataaat gaacccggtt gatcgtccac tattaaatat tggcttaacg cgattagaat
      301 tcttgcgtat atcaggaaaa ggccttgctg gtttaacaat tgcccctgcg ttactgtcac
      361 ttttgggttg caaacaggaa gatattgata gcggcacagt agggttgata aatacaccca
      421 aaggggtgct ggtgacccaa cgtgcgcgat gcacgggttg ccaccgctgt gaaatctctt
      481 gtaccaactt caacgatggc tcagtaggga cattcttctc ccgtatcaaa atccatcgca
      541 attatttctt tggcgacaac ggggttggct ctggcggcgg cctgtatggc gatctcaact
      601 ataccgcgga tacctgccgt caatgcaaag agccgcaatg catgaacgtc tgcccgattg
      661 gtgcgattac ctggcagcag aaagaaggct gtattaccgt cgatcataaa cgttgtattg
      721 gctgtagcgc ctgtactacg gcatgcccgt ggatgatggc caccgtaaat accgaaagta
      781 aaaaatcctc gaaatgtgtg ttatgcggtg aatgcgcaaa cgcctgccca acaggggcgt
      841 taaaaattat cgagtggaaa gatattactg tgtgaatctt gcaaaggaaa aaatcatggc
      901 taacggttgg acaggtaata tattaagagt caatctcacg acaggaaata ttaccctcga
      961 agattccagt aagtttaaaa gttttgtcgg tggcatgggc ttcggctaca aaattatgta
     1021 tgacgaagta ccgccaggca cgaaaccttt cgatgaagcg aataaattag tttttgctac
     1081 cggcccatta actggatctg gtgccccctg tagttctcgc gtaaatatca cctcactttc
     1141 tacttttacc aaaggaaatt tagtcgtcga tgcccatatg gggggctttt ttgcagcgca
     1201 aatgaaattc gctggatacg acgtcattat tatcgaaggg aaagcgaaat caccggtatg
     1261 gctgaagatt aaagatgaca aagttagcct tgaaaaagcc gatttcttat ggggaaaagg
     1321 gacgcgcgca acgactgaag aaatttgtcg cttgaccagt ccggaaacct gtgtggcggc
     1381 tattggtcag gcaggggaaa accttgttcc tctctctggc atgttgaata gccgtaacca
     1441 cagcggcggt gcgggaactg gcgcaataat gggttcgaaa aacctgaaag cgattgcggt
     1501 tgaagggacg aaaggggtca acattgccga tcgtcaggag atgaagcgtc tcaacgatta
     1561 catgatgact gaacttatcg gtgcgaataa caaccatgtc gtgccaagta cgccgcaatc
     1621 gtgggcagag tattcagatc ccaagtcacg ttggacagca cgtaaagggc tgttttgggg
     1681 cgcggctgaa ggtggtccga ttgaaacggg ggaaattccg ccaggcaatc agaacacggt
     1741 cggctttcgt acctataaat ccgtttttga cttaggtccg gcggcagaga aatacacagt
     1801 aaaaatgagc ggctgccact cttgcccgat ccgttgtatg acccaaatga atattcctcg
     1861 ggtgaaagag tttggcgtgc ccagcacagg tggtaacact tgtgtagcaa actttgtcca
     1921 taccaccatc ttcccgaacg ggccgaaaga ttttgaagat aaagacgatg gtcgtgtgat
     1981 tggtaacctg gttggtctga atttgttcga tgactacggc ctatggtgta actacgggca
     2041 gttgcatcgc gactttattt attgttacag caaaggtgtg ttcaagcgtg ttctgcctgc
     2101 tgaagagtat gcggaaattc gctgggatca actggaagcg ggtgacgtta actttattaa
     2161 agatttttac taccgtctgg cgcatcgcgt gggtgagttg agtcacctgg ctgatggttc
     2221 atatgccatc gcagaacgct ggaatttggg tgaagagtac tggggctacg cgaaaaataa
     2281 actctggtcg ccatttggct atccggttca ccatgccaat gaagcgtcag cgcaagttgg
     2341 ttccattgtt aactgtatgt tcaaccgtga ctgcatgacg catacccata tcaattttat
     2401 tggttccggc ttgccattga aattgcagcg tgaagtggcg aaagagcttt ttggttctga
     2461 agatgcttac gatgaaacca aaaactacac accgatcaat gacgcaaaaa ttaaatatgc
     2521 taagtggtcg ctgttgcggg tctgtttgca taacgccgtc accctgtgca actgggtctg
     2581 gccaatgacc gtttcgccgc tgaaaagccg taattaccgg ggcgatctgg cacttgaagc
     2641 caaattcttc aaagcgatca ctggcgaaga tatgactcag gaaaaattag atttagctgc
     2701 agagcgtatc tttacgttgc atcgcgccta cacggtaaaa ctgatgcaaa ccaaagatat
     2761 gcgtaatgaa cacgatctta tctgttcctg ggtattcgac aaggatccgc agatcccggt
     2821 ctttactgaa ggtacagaca aaatggatcg tgacgatatg catgcttcgc tcacgatgtt
     2881 ctacaaagaa atgggctggg acccacagct tggttgtccg acccgcgaaa cattgcagcg
     2941 tctggggctg gaagatattg ccgccgatct ggcagcacac aatctactgc ctgcgtaagg
     3001 aatgggagag atgaaccatc aggatgaatt gccgttagcg gaagtcagcg aaattgatga
     3061 ggcgaagcgg caatggttgc aggggatgcg tcaccccgtt gatacggtaa ccgaaccaga
     3121 accggctgaa atcctggcag agttcattcg ccagcattct gcggctgggc agctggtggc
     3181 gcgcgctgtg tttctctcgc cgccctattc ggttgcagaa gaggaactgt cagttttgct
     3241 ggaaaacatt aagcagaacg gagactacgc ggatatcgct tgtatgacgg gaagccagga
     3301 tgattactat tactcaacgc aggccatgag tgaaaactac gcggcgatgt ctttgcaagt
     3361 tgtggagcag gacatttgtc gcgccattgc acacgccgtt cgttttgagt gccagacgta
     3421 tcctcgtcct tacaaagtcg ccatgctgat gcaagcgcca tattacttcc aggaagcgca
     3481 gattgaagcc gcgatcgccg cgatggatgt tgcacctgaa tatgccgata tactgcaggt
     3541 ggagtcatcg acggcagtgt tgtacttgtt tagcgagcga tttatgacct atggcaaggc
     3601 gtatggctta tgcgaatggt tcgaggtaga gcagtttcag aacccctgaa atttcagggg
     3661 gatgtcatgg atagggtgga cagttgcagc cactgcatta ggggataatc agatgtcctt
     3721 cactcgacgc aaatttgttc tggggatggg aacagtaatc ttttttactg gttctgcgtc
     3781 ttctctgtta gcgaatacga ggcaagaaaa ggaagttcgg tatgccatga ttcatgacga
     3841 gtcacggtgc aatggctgta atatctgcgc ccgcgcatgc cgtaaaacta accacgtccc
     3901 cgctcaggga agccgtttat cgatagcaca cattccagtc atcgataacg acaatggaac
     3961 gcagtatcac ttttttcgcc agtcatgtca gcactgcgaa gatgctcctt gcatcgacgt
     4021 ttgcccgaca ggggcgtcgt ggcgtgatga gcaggggatc gtgcgggtag aaaaatcgca
     4081 gtgcatcggc tgcagttatt gcattggcgc ttgcccgtac caggttcgct accttaaccc
     4141 tgtcactaaa gtggcagata aatgtgattt ctgcgctgag acccgactgg caaaaggctt
     4201 tccgcccatt tgcgtcagtg cctgcccgga acacgcattg atctttggtc acgaagatag
     4261 cccagaaatt caggcgtggt tgcaggaaaa taaatactat caatatcagc tacctggcgc
     4321 cgggaaaccg catctgtatc gccggttcgg tcaacatttg attaaaaagg aaaatgtatg
     4381 aacccgtcgc aacatgctga acagtttcag agccagttgg cgaactatgt gccacagttc
     4441 accccccaat tttggccggt gtggttgatc attgccggac tattgctggt tgggatgtgg
     4501 ttggtgctgg ggctacatgc cttgcttcgt gctcgtggcg tgaagaaatc agccaccgat
     4561 catggtgaga ggatttatct ttacagcaaa gcggtcagat tatggcactg gtcgaatgcg
     4621 ttactctttg tattgttgct ggcaagtggg ctgataaatc actttgcgct ggtgggcgca
     4681 actgcggtta aaagtctggt tgcggtgcat gaagtttgcg gatttttgtt actggcatgc
     4741 tggcttggct ttgttctgat caatgccgtt gggggtaatg gtcaccacta tcgcattcgt
     4801 cgtcagggtt ggctggatcg agcggcaaaa caaacgcgat tttatttgtt tggcattatg
     4861 cagggggaag aacatccttt cccggcaaca acccagtcta aatttaatcc cttacagcag
     4921 gtcgcctatg ttggtgtcat gtatggattg ctgccattgt tactattgac ggggctgctg
     4981 tgtctctatc cgcaagccgt gggagatatg tttcctggcg taagatactg gttattgcag
     5041 gcgcattttg ctctggcatt tataagcctc ttttttatct tcggtcatct ttatctttgc
     5101 accacggggc gtacgccaca cgaaaccttt aaaagcatgg tcgatggcta tcaccggcac
     5161 taacgaatga tcattacacg agcagacctg cgagaatggc gtatcggagc ggtgatgtat
     5221 cgctggtttc tgcgccattt cccacgcggc ggtagttatg ccgatattca tcatgcactg
     5281 attgaggaag gatatacgga ctgggcggag agtctggttg agtatgcgtg gaaaaaatgg
     5341 ctggcggatg aaaacttcgc gcatcaggaa gtttcttcta tgcaaaaact ggccacagat
     5401 cccggagaga gagcattttg cagtcagttt gcccgcagcg acgatcatgc tcgcatcggc
     5461 tgttgtgagg ataatgccag aatcgcaact gctgggtatg ccgcgcaaat tgccagtatg
     5521 ggttatagcg tgcggattgg aagtgtcggc tttaacagtc acattggcag ctccggtgaa
     5581 cgcgcacggg ttgctgtaac cggtaattct tcgcggataa gcagcgctgg agatagtagt
     5641 cggattgcga ataccggaat gcgagtacgc gtttgtacat tgggtgaacg gtgtcatgtc
     5701 gccagtaatg gtgacctggt gcaaatcgcc agctttggcg caaacgccag aattgccaac
     5761 agcggcgaca atgtacacat cattgccagc ggtgaaaatt caacggtagt gagcacgggc
     5821 gtggtcgact caattattct cgggccagga ggaagcgccg cactggcata tcatgacggc
     5881 gaaagagttc gcttcgccgt cgccattgaa ggtgaaaaca atattcgagc cggagtccgc
     5941 tatcgcctta atgaacagca tcagtttgtt gagtgctgat agctttacat ttgattaaac
     6001 gaaagacgcc gacgagcacg ggatgcaggt tttacgaccg cccgagccat cgcctctcca
     6061 atttctgcac atgccgtaag tccctgaacg aaaggctggt cgtgcatcaa ccagggcaac
     6121 gcgccgaacg ctacgcgccc acgaatatct tcgggttgct gtaacgtata atcttcgcca
     6181 acatcaggga tttcatcccc tgttttctgt aattgttcgc gtagtccagg gaaaggaata
     6241 tctttcactt taagcggacg ctgcccacgg gcatcaataa aaacgtcaaa cgagtagcta
     6301 ttgtcttccg ttttcaggac ggtgcgcgac tcattaattt ccatttcgta gtcttcaccg
     6361 agggcgagaa tatgaatgat tcccgcttca cgtaaggcca gtagtcgacg aatagactct
     6421 gaagggattg ccgcataatt atcgatgaaa acccgggcaa ggcctttact gaaccgttta
     6481 tggtcgtgtt cattcagatg aggaacaatt tcctgtacgg cttcatgcag gcgcagaatg
     6541 acataacgcc agggaacagt atgattttct cgtttattgc gttcgacctc ctggagattt
     6601 ttttctgccc agtcgaatgg gtcgcgttgt ttgcgctcgg caaaccaggc ttgagcaaag
     6661 gaatcgacat tcaggctttc taaggctatg cgttggctcc agtctggatc agcaaatttg
     6721 atttcctcta ctatcaatct aaatacccga tccaaaaggc cctcttcgcc tttttgaatc
     6781 tcagcattta atgcttgatc agtgacgatg tgtaagggct cgtagggaat agggcaatag
     6841 aaatcggctt cgggtaaaat acctgtgcgc gacattaacg taatatttag cttttcactt
     6901 gcgttatcgc ggtgaaaaat gacgtgttgt ttatcatctt caatgaacga accatgctga
     6961 atagccactg ccattgccgc atccagtccg ctcaaggatg ttcccataat acccacgtta
     7021 cacgcatcga cctttgcttc catcaggcct gaccacgggc tgggaaaata cgttctggtt
     7081 gcttcttctt catcaggcca gacgtgcccc gttgcgatca ccactaaatc aaacgtctcg
     7141 ctgggtaaat ccagatttgt agcgagcatg acgccagcat ttgtaatttg cagatcggta
     7201 acctggcatg attcataaac agccactgca aatttttgct gtcgtgcctg gtctactaat
     7261 cgtaaaaatt gatcgcggaa atattcgccc agcagaattc gcggtaaaaa ctgacgatcg
     7321 tgcaaagttt cttttttaac gccataacgt tggagatggc tggcttcttg cttttgtagc
     7381 cattcgagat acgtacaata aatcggcggt atttcaatac tggcaatatt tgccagcatc
     7441 agttttgagt tttcctcatc actgtatggc atcccgacac cggcttcatc agcctgctcg
     7501 aagatagaaa tagaaagtgg cgtttgttgc tgtagaagcg agaataaggt gtagatcccc
     7561 gtaggcccgg cacccacaat agcaattttt ttcattgtta cttacctgta cgcattcttt
     7621 cgaatttagt gttatcagat aagtttggtt gagattttta gaatttgaga aatttcagac
     7681 agggatgaaa agggcgctaa ccgcccttga ttaaattctg tcaggcgagt tttgcctgat
     7741 tgatttgact cagtggctca ttgacatcga aaactttggc gacaacttcc tcaacgccga
     7801 gatttttcag tcgggcagta tgtttttcca gataggcaag ggcgcttttt tcatcagtaa
     7861 aaaggtagat cccaccggct tcgtggttct tttcactttc tgtccatacc ttccacagaa
     7921 aaccaggttc ctgattaatc gactcagcaa gaggtttaag ctgctcagcc attgcgtcgc
     7981 caaatgggcc attgaaagaa aaatgaagtt gtaacaaagt tgccataacg attctccttc
     8041 tgataacgca tacgaagaca gtgtaaatct tttaacgtat tggatacaga gaagaaacat
     8101 agggaaaaaa ggagaagacc agattaagaa aatctggtgc gtccgagtgg actcgaacca
     8161 ccgaccccca ccatgtcaag gtggtgctct aaccaactga gctacggacg caggatggtg
     8221 cgttcaattg gactcgaacc aacgaccccc accatgtcaa ggtggtgctc taaccaactg
     8281 agctatgaac gcaacgttgt aggtgacaac ggggacgaat attagcggca gagtgggaag
     8341 gtggcaagag gcaaaacgta attttctgcg ctatttcgac cgtttgcaga gcttttaagc
     8401 aaattggcta tattttgttg atttgcaagg gtgattttta ttcaggatcg catttacatc
     8461 tgacacaacc caataatccg tgtatcagag tttaaacaga gttgtttgcc accatctcaa
     8521 atctgtcgtg gataaatcct tcgtagcctt tacacttagg tagcaataaa ggcacacaga
     8581 tgcagcaaag gaataaatca tgggatctga tgcgaaaaac ttgatgagcg acgggaacgt
     8641 gcaaattgtt aagaccggcg aggttattgg cgcgacgcaa cttactgaag gcgagttgat
     8701 tgttgaagct ggcggaagag ccgaaaatac cgtggtcacg ggggctggct ggttgaaagt
     8761 ggcaaccggt gggatagcca aatgcacaca gtacggtaac aatggcacac tatcggtcag
     8821 cgacggtgcc attgccacag atattgttca gtccgaggga ggcgcaatta gtctctctac
     8881 tctcgctacg gttaatggcc gccatcccga aggtgaattc agcgttgata aaggttatgc
     8941 ctgcggtttg ttgctggaaa acggcggtaa cctgcgtgta ctggaaggcc atcgcgcgga
     9001 aaaaatcatt ctcgatcaag agggcggcct gttggtcaat gggacaacct cagcggtcgt
     9061 ggtagatgaa ggtggtgaat tgttggtgta tccaggtggg gaagcctgca attgtgagat
     9121 taatcagggc ggcgttttta tgctggcggg gaaagccagt gatacgttgc ttgctggtgg
     9181 caccatgaat aatctcggtg gtgaagactc tgacactatt gttgagaatg gatccattta
     9241 tcgtctgggg acggatggta ttcagctcta cagttccggt aagacgcaaa acctgtccgt
     9301 taatgtgggg ggtcgggctg aagtgcatgc tggtacgctg gaaaacgcgg taatacaagg
     9361 tgggacagtg attttgttgt cacccaccag cgcggacgaa aattttgtcg tagaggaaga
     9421 tcgtgcaccg gttgaactga ccgggagtgt tgtattactg gacggcgctt caatgattat
     9481 tggctatggc gcagagctgc aacaatcaac gattactgta cagcagggcg gtgtgttgat
     9541 tctcgacggc agtacggtaa aaggtgacag tgtcactttt ggtattggta acatcaatct
     9601 gaatggcgga aaactgtggc tgatcactga tgcggcaacg caggtgcaac tgaaagtgaa
     9661 acgcctgcgt ggagagggag ccatttgcct gcaaaccagt gcgaaagaaa tttcacctga
     9721 cttcatcaat gtgaaagggg aagttaccgg tgatatacac gttgagataa cagatgccag
     9781 tcggcaaact ctgtgtaact ctctgaaatt acagccagac caagatggca tcggcgcaac
     9841 gctccagcct gcgtaaaaac acaggcgcaa cccgtgaagg ttgcgccttg tctttattag
     9901 cgggatgctc gttgcagaat gatggctgac ggcagacgtt gcaggaaccg catacgcagc
     9961 atcatcataa tggctgccga cgtcaggcca ataataaagc ctatccagaa gcctgctggc
    10021 cccataggtt caacgaccag atcggtcagt gccagaatat agccgcttgg caagcccagc
    10081 acccagtaag ccgtaaaggt gatatagaaa atggaacgcg tatctttata accacgcaaa
    10141 attccactgc caatcacctg gatagagtca gaaatctgat ataccgccgc cagcaacatc
    10201 aaatgtgcag ccagcgttac aacctcggga ttgtcgttgt acaacagggc gatttgctcc
    10261 cgcagtgaaa ccgtgaatat ggccgtcagg gttgccatac agacacccac cataagcccg
    10321 gtccgcgcag cggtttgcgc atccagcgtt gagccttgac ccagacgata acctacgcgg
    10381 atggttaccg ctgccgccag cgacatggga agcacgaaca tcagtgaact aaagttcagg
    10441 gcaatctgat gtcctgcgac atcaacaata ccgagcggag acactaacag agccacgacg
    10501 gcaaacagcg ttacttcaaa gaacagtgcc agcgcaattg gcaaaccgag ttgaatcagt
    10561 cgtttcataa ccgcaggatc gggcttcgcg gtgccttttt cgttacgaat atcgcgcatc
    10621 gagcgggcgc gtttaatgta agaaaccatg gcaaggaaca tgacccaata caccgccgca
    10681 gtagccacgc cacaaccaac tccaccgagc tcaggcatac cgaaatgacc ataaataaag
    10741 atatagttaa ccggaatgtt caccagcagg ccgataaagc ccatcaccat acccggcttg
    10801 gtttttgcca gaccttcaca ctggttacgg gcaacctgga agaaaagata gcccggcgcg
    10861 ccccatagca acgcacgcag ataacccacg gctttgtccg ccagagccgg atcgatgttt
    10921 tccatggagc ggataatgta gcctgcattc cacagcacca gcataatgag aacggaaaca
    10981 aaacccgcca gccagaaacc ttgtcgcacc tgatgcgcaa tgcgctcacg tcgaccggaa
    11041 ccatttaatt gcgcgataac cggcgttaat gccagcagca gtccgtgacc aaagaggatc
    11101 gccggaagcc agatagaagt accgatagcg accgccgcca tgtcggtggc actatagccg
    11161 cccgccatca cggtatcgac aaaacccatc gcagtttggg cgatttgcgc gagaatcacc
    11221 gggattgcta atgctaataa cagacgcgct tcactgatat acttctgcac gtgaacacct
    11281 tttatttgta gttatatgag agactaaaaa gccgcctgaa tgggcagcaa aaagaagaag
    11341 caggggaaat tcagtctatt gtagcgagga tttattaatt ctccagtgaa aaaatagcca
    11401 ttcttacccc ttcactggta agggcgtttt ccacctgcta ttgtgctgaa cagaatgtct
    11461 taactgattt caggagttgt aagtatgttt acggggattg tacagggcac cgcaaaactg
    11521 gtgtcgattg acgagaaacc aaattttcgc acgcatgtgg tggagttacc cgaccacatg
    11581 ctggacggtc tggaaaccgg tgcttccgtg gcgcataacg gttgctgcct gaccgtgacg
    11641 gaaattaacg gcaaccatgt cagttttgac ctgatgaaag aaacgttacg cattactaat
    11701 cttggcgatt taaaagtggg ggattgggta aacgttgagc gtgcggcgaa attcagcgat
    11761 gaaattggcg gacacttaat gtcaggtcat attatgacca ccgctgaagt agcgaaaata
    11821 ttaacctcgg aaaataatcg tcagatctgg tttaaagtcc aggatagtca gttgatgaaa
    11881 tatatcctgt acaaaggatt tattggcatc gacggtatta gcctgaccgt cggcgaagtc
    11941 acgccaacgc gtttttgcgt ccatttaatt ccggaaacac tcgaacgcac gactcttggg
    12001 aagaaaaaac tcggcgcacg cgtcaacatt gaaatcgatc cacaaactca ggcagtggta
    12061 gatacggtag aacgtgtgct ggcggcacga gaaaatgcca tgaatcaacc aggtacagaa
    12121 gcctgatctg aaatcgcccc cggagagggg gcgatagaat agcctttagc gagcgactcg
    12181 aaggccgttt tcaacaccgc gtgagaacac gacctgccag agctgaatat cacgggcgcg
    12241 gaaagcacct gcacaggcat tcagataata ggtaaacatc cgtttaaagc gttcactgta
    12301 gttatccgca atttctggcc atgcggcgag gaatcgttca taccacgcca tcaatgtagt
    12361 atcgtaatca gcaccgaagt tatgccagtc ttccatcaca aagtggggtt cgctggactg
    12421 agcaatctgg cgtacagagg gcaggcaacc gttcggaaaa atatatttat taatccaggg
    12481 atcaacattc agatcggttt ttttcgaacc gatggtatgg agcaggaata taccttccgg
    12541 tttcaaatta cgatccacca ccgcaaaata ggtatcgtaa tttttcggtc cgacgtgttc
    12601 gaacatcccc acagaaacaa tacgatcaaa ctggtcgttc aggtcacgat aatcttgcag
    12661 caaaatggtg acatccaggc cttcacagtg ttcctgagcc attttttgct gttcggcaga
    12721 aatggtgacg ccgaccacgc ttacgtcata attagacgcc atgtagtgcg ccagtccacc
    12781 ccagccacag ccaatatcca gtacgcgcat ccctggtttt aactgcaatt tttcacaaat
    12841 cattttgagc ttcgcctgct gggctgattc cagattatcg gcatctttcc agtaagcaca
    12901 ggaatattgc atgaagggat caagcatgcg gctgaacaag tcattaccca aatcgtaatg
    12961 ctctttgccg actatccagg cacgtttttt actctgcaga ttgaagagac gagcgccggc
    13021 aatacgtagc gtgtctttga aatgatgggg aagttggttc tcgagacctg cgcgtaagac
    13081 tttgctaaaa aacatatcca gtcggtcaca ttcccaccag ccatccatat aactttcgcc
    13141 taaccccaaa gaaccttcct gcagaacgcg tttaaaaaaa tcggggtttt tgacacgaat
    13201 atccgccggg gcagaaccgt taatggctat accggcacgg ctaagtaatt cgttggcgat
    13261 acggtaccag ttgtcatccg gtacactgac ttcttctata cacgatgaac tcatagtttc
    13321 tccatcactg gtcggtgatc agaaccttaa aacagcgtag acgctttttg ggctttgtga
    13381 gaaatctcgc agaaaaaacc gcgagtctat aatccgacgt agaacagagg gaaaggagga
    13441 acccttgccg aaatgaccat ccatagtgag tcgggaacga tcctgtcccc gtaataacat
    13501 aatatttatc gtagttttaa caactcgaaa ttagtatagg ccgctaattt caatcataca
    13561 acaaaatttt gttagcagcc taagtataag tatatcgata tagatcagtg tgattcgcta
    13621 tgagcgactt cggcattgcc atgattctgg cagccaactt cttcacaacg ttgcatcatg
    13681 taacccagag cgaccagcac tactgttgat aacatcacgc tggtggtggt gagcaatggc
    13741 gtgctgatac tgataagcca ggaaactacc agacttgcga ggaagcacag acccagttgc
    13801 agagtgttct gcaacgctgc ggcgcgaccg gttgcatgtg ggaagggacg cagcgcctgg
    13861 gcgacaacaa tcgggtagat cgcaccgttg gcaatcgcca tcacacagaa tgggatcagg
    13921 atttcgacca gcgacacatg gctaatgaag cccgcagccc aggtcgcaat gacgctgaca
    13981 gcaaacagta ccagcaacca cggtaataac tgcttgcctt gccatttctg cagcgcggca
    14041 cgacagccat aaccaccaat cagaaacgcg atagtttgcg ggacataact taaaccaata
    14101 actgccggac tgtaacccat ttcactaagg atgaacggtg aaccggtcag ccatgcaaaa
    14161 aaactggctg aacaggccgc gtatatcagc acgttgccgc gataggtttt agaacgtagc
    14221 aggtcggtaa aggtcagacc atcctgacta ttgttacggg ccttcgtcgt gggtttgagc
    14281 cagaaaatag gcagaatcag caccacggta atggcaaaca gggtggcgaa aatcgcctgc
    14341 caggaaaaat ggaccagcag ccagcttcct aacagaggag ccaatgccgg agacagaccc
    14401 accagcggca tgatggtcgc aaaaatacgg ttaactttct gtgaaggata ataatctgtc
    14461 actaatgctt gccagataac cgccgcggcg cagacaccca cagcctgtac aaaacgcaat
    14521 atcagcagcg tggcggcgtt ttctacccac agcatcccca gactacctaa cgcaaaaatt
    14581 gtcaggccga tgaataacac cggtttacga ccataacggt cggagagtgg cccccacaga
    14641 agctgggctg cggcaaaacc ggcaaggaac agactaaggc tggcactgac agcagacgca
    14701 ggcgtttgca ggtcggcctg tatggcggcg aaagcaggca gatacatatc ggttgccaga
    14761 aaaccgagta cgcttaaacc tgccagccag actaaaaatc ttttcccagg ttgcatgtat
    14821 attctctaaa tctctcaatt aaacaccgcc gcaaagtgta gggagtgaaa ttagtgttgt
    14881 gaaacggtaa tatttgatga ttggtttcaa aaattttgca ggcagactat gtggtcagaa
    14941 tattctctgg aagttgttga tgcggtagcg cgtaatggta gttttagcgc cgcggcccag
    15001 gagttgcatc gcgttccttc tgcggttagc tataccgtgc gtcagctgga agagtggctg
    15061 gcggtgccgc tttttgaacg acgtcaccgt gatgtggaac tgaccgctgc cggggcgtgg
    15121 tttctcaaag aaggacgctc tgttgtcaaa aaaatgcaga tcacccgcca gcaatgtcag
    15181 cagatagcga acggctggcg cggtcagtta gctatcgcag tggataatat tgtcaggcca
    15241 gagcgaacac ggcagatgat cgttgatttt tatcgccatt ttgatgatgt cgaacttctt
    15301 gtctttcagg aagtgttcaa cggtgtctgg gatgcacttt ccgacgggcg cgtggaactg
    15361 gcgattggcg caacacgcgc gattccggta ggcggtcgtt ataccttccg ggatatgggg
    15421 atgttaagct ggagttgcgt tgttgccagc caccacccgc tggcgttgat ggatggcccg
    15481 ttcagcgatg atacgttgcg caactggccg tcattggtgc gcgaagacac ctcgcgaacg
    15541 ctacctaaac gtattacctg gttgctggat aaccaaaaaa gacttgtcgt gcctgactgg
    15601 gaatcatcgg caacctgtat ctcggcagga ttatgcatag ggatggtgcc aacacatttc
    15661 gccaaaccgt ggcttaatga ggggaagtgg gtagcactag aactggaaaa ccccttcccg
    15721 gattcggcat gttgtctgac atggcagcaa aatgatatgt cgcctgcgtt aacctggttg
    15781 cttgagtatc tcggcgatag tgagacgctg aataaggagt ggctgcggga gccggaagag
    15841 actcccgcaa cgggtgatta acgacgatag tcgcggaacg ggccgtcagc cacggagcgg
    15901 cgttcaatca accgcggatg cacttcaatg gactgcggtt cttcacgttt gttgacgata
    15961 cgatccaaca gcatgttgaa cgctgtttcg cccagcgaat cttttggctg atggatcgtg
    16021 gtcagcgccg gcgtaaaata gcgggcgttg cgcacgttat cataaccgat cagcgaaaca
    16081 tcctgcggca cgcgcagacc catttcatca gcagcacaaa gtgcgcccat tgccatgata
    16141 tcgccaccac agaagacggc agtagggcga tgcgactgcg acaggatttg ctgcatggcg
    16201 cgataaccgg attcaggttc aaagtcaccc tgcacaatcc agctttccgg cactttgatc
    16261 atcgcttctt ccatcgcctt cataaaaccg gcaaggcggc ctgcgccggt gttacgttcc
    16321 agcgggccag ggataacgcc gatttcgcga tgaccgcgtt caatcagata acgcccggcc
    16381 atgtagccgc cttcgaatgc gttatcaatg accgcatcgg tgaagtcagc ttttgcttca
    16441 ccccagtcca tcaccaccat tgggatatgg cgatactctt ccagcatcgc cagcaacggc
    16501 tctgggtact cagaacacat caccagcaga ccatcgacgc gtttttgcgc catcatcgac
    16561 agataagccc gctgtttctc aagattgttc cacgcattgc ccagaatcag ggtgtaacct
    16621 ttctggaagc aatttttttc gaccgcttca atgatctcgg caaaataggc cgcttcgctg
    16681 ctggtcgcca gcaaaccgat agacttggtg tggttaacct tcaggctacg cgccaccgcg
    16741 ctaggggagt agtgtaattc tttgattgct gcccacacgg cattgcgcgt ttcttcagcg
    16801 acgaaacgtg ttttgttgat cacgtgtgac acagttgtag tggaaacgtt tgctcgtttc
    16861 gctacatctt ttattgttgc cattccattt cactccagac cctaacttca gctcctgcaa
    16921 attcgcaagg taaacgtttg cctttacaca ccttacgcac aacaaaaatg ttgcggtacg
    16981 ccggaaaaac gacacggagc gtcaggaagg ggtcaatggc cagtacgcaa ataatgtaag
    17041 cgtggaattt tggccgatct tgacgaaaag gggaagtagt aaaaacactt atcattataa
    17101 atgacgggag attttttcat cacagtgtgt aaaaatgcgt aatatcactt tttgtgtggt
    17161 aataaaaaag gagaaaactt gatgagcacc gaccttaaat tttcactggt aacaacgatt
    17221 atcgtcctcg gtttgatcgt ggccgtgggt ttgactgccg cgctgcactg atttcctctc
    17281 gagggagcgc gttctccctc gcatctcctt cctgtttgtt agcaaaatgg caaaagtgtt
    17341 ttctcttttt gttattttca ttttttgtga ttactgtcat gctttcgcca ctttaatgaa
    17401 tatcgcgtaa tgacgctaag aggtggagat aaggcatgaa aattaactat ccgttgctgg
    17461 cgctggcgat tggcgcgttt ggtatcggga caacggagtt ctcgccaatg ggcttgttgc
    17521 ccgtcattgc gcgcggtgtg gatgtctcga ttcccgctgc cggaatgtta atcagtgctt
    17581 atgcagttgg cgtaatggtt ggcgcgccgc tgatgacgct tctactttct catcgtgccc
    17641 gccgcagtgc gttgattttc ctgatggcga ttttcacgct tggcaacgta ctttccgcca
    17701 tcgcgccgga ttatatgacc ctgatgcttt cacgcatttt gaccagcctg aatcacgggg
    17761 cattttttgg tttgggttca gtcgtggccg caagcgtagt gccaaaacat aaacaggcta
    17821 gcgcagttgc cactatgttt atggggttaa ccctggcaaa tatcggtggc gtgccggcgg
    17881 cgacctggtt gggcgaaacc atcggctggc ggatgtcatt tctggcaaca gcggggctgg
    17941 gcgtgatttc aatggtaagt ctgttcttct cattacctaa aggtggcgca ggggcgcgac
    18001 ctgaagtgaa aaaagagctg gcggtattaa tgcgtccgca ggtgctgtct gcattgctga
    18061 cgacggtact gggcgctggt gcaatgttta ccctctacac ctatatctct ccggtactgc
    18121 aaagtattac ccacgtaacg ccggtgttcg tcacggcaat gctggtgctg attggtgtcg
    18181 gattttctat cggtaactat ctcggcggca aactggcaga tcgttcagtt aacggcacgt
    18241 tgaaaggctt tttgttgttg ctgatggtga ttatgctggt aatcccgttc ctggcccgca
    18301 atgagttcgg cgcagctatt agcatggtag tgtggggagc tgcaaccttt gcggtcgtac
    18361 cgccgttaca gatgcgcgtg atgcgtgtcg ccagtgaagc gccaggtctg tcttcatcag
    18421 tcaatattgg tgcctttaat cttggaaatg cgctgggagc agctgctggt ggtgcggtaa
    18481 tttccgctgg gctgggatac agttttgtgc cggtgatggg ggcgattgtc gcgggactgg
    18541 cattattgct ggtgtttatg tcagtcagaa aacaacctga aacagtttgc gttgccaaca
    18601 gctaattaaa caaaaacgca gaaggataac cttctgcgtt acagttgctg gcaacgtgcg
    18661 atttatcttg ccgaatgcgc tgttaacgcc tcatccgcgt tggcatggta agcgaagcgc
    18721 atcagataat gttgcatttg ccatcagtta ttatgcagcg agatttttcg ctacgaattc
    18781 ccagttcacc agcgcccaga agtgctccag atagccagga cgtgcattgc gatagtcgat
    18841 gtaataagcg tgttcccaga catcaacggt cagcagcgga gtcgcatcgg tggtcagtgg
    18901 agtacccgcg ttagaggttg aaacgatagc cagtttgcca tcgctgtttt tcaccagcca
    18961 ggtccagcca gaaccaaagt ttttgatcgc tgcatcagta aactgagctt tgaaatcggc
    19021 aaagctgcca aaagatgcgg cgatagcttc agcgactttt ccagtcggtt cgccacccgc
    19081 gttcggtgcc aggcagttcc agtagaaagt atggttccag acctgagctg cgttgttgaa
    19141 tacgccacct tcagagctgc gaataatctc ttccagtgat ttaccttcaa acgcggtacc
    19201 tttgatcagg ttgttcaggt tagtgacata agtctgatga tgcttgccgt agtgatactc
    19261 gatggtttcc gcagaaatgt gcggtgccag agcatcttta gcatatggta gtgcaggtaa
    19321 ttcgaatgac attgctactc tcctttatta ttaatttgca tacgtacaat agccttattg
    19381 tgcgtatgga tagggtagca aattaaaagg tgagacaaaa gatgaactta ccctgttgcc
    19441 gtaacaacag ggtaaagttt tagcgaagtg tttttggggt cattacccgg cgagcgccaa
    19501 catagtggcg ctgccagtag tcttcactga gagaagtgat ttgaatttct tggcccgtgc
    19561 gcggtgactg aataaatttg ccgttgccga catacacgcc gacatgatcg gctgtaccgc
    19621 gtccctgagt acggaagaag accaggtcgc cgtttttcag ttcactacgt tcgattggcg
    19681 ctgcatcgcg caggtgatac atttcattcg ccgtacgcgg aatacgaatt ttcaccaaat
    19741 ctttataagc gtaataaacc agaccgctgc aatcaaaacc ggtacgcggt gaactgccac
    19801 cccaacgata tggcttacca atttgctgca tcagtttatt cattgccact tttgtggctt
    19861 tttgcacctt cgctttgtgc gcgtcagcaa gggttcctga cgtggcattt ttgactttta
    19921 cacaatgcga tttatagccc ttacgtttgg tacacttttc ggtaaagctg gctgaggccg
    19981 ttttattgac ggcgtgtttg ctgcgagttt tggcgttttt agaagcggta atggaagatt
    20041 ttttaacgga agaagcggtt ttgcttttgg tcgtactttt actggcggct tttttcgctg
    20101 ttttctggct ggctttctgg gtcttttggg tggttgccgt gcttttcttt ttatctgctt
    20161 ttttggtgat atgagtggta gcagaactct ccctggcttg ctttgaagca tgggccatag
    20221 gcgttaaagg cagggtggta aaaagtaaag cacagagcgt gatcgaaata cggtttatcc
    20281 gcgccactga gcgtccctct gttaaggcag gtctatcatg cctgcgtctg atttacaaaa
    20341 cttgatgatt ctaatgcata gaagaagaga aagttaatag cttttattgt taaaaaatac
    20401 gtttcgtcgg cgagcgcaaa aaaatgcagc agtgaaagct aaaaatggtt ataaatacgc
    20461 cagtttgccg tgcatcaggg aagaaacgaa tatttgaaat gcaacaaata gagcaaaggg
    20521 ataaaatagc aaaagcgtga tgaaaatgtt attttccgat gccgcactga cccgctacaa
    20581 tgtcagatta ctgccgtaaa gaagtttgag gaagcaagac aatgagcacc actatcgaaa
    20641 aaatccaacg ccagattgct gaaaacccga tcctgctgta catgaaaggg tcaccgaaac
    20701 tgccgagctg cggtttctct gcccaggcag tccaggcgct tgccgcatgt ggcgaacgtt
    20761 ttgcctatgt tgatattctg cagaatccgg acattcgtgc ggaactgccg aaatatgcga
    20821 actggccgac cttcccgcaa ctgtgggttg acggcgagct ggtgggcggt tgtgatatcg
    20881 tgatcgaaat gtatcagcgt ggtgaactgc agcagctgat caaagaaact gccgctaaat
    20941 acaagtctga agagccggac gcggaataat ccgtcctgca atactgacgg atgcgaacgg
    21001 gcatccgtca gtcctttctc tatccccaat ccagcccttg tggcgaactt gaaaatcccg
    21061 cctcacgtaa ccgcgtaaac atcggcgttt gccggaccgg taggtcattc acttctgtta
    21121 gcgtaaagcg cagccgtggt tcgcgactgg acagctgtcg gctgtttaaa ggcgcgaaga
    21181 aaccagtcgc gggtcgccgg tgaaaacacg tccggcaggg gcgatgaagg gtttggatta
    21241 tttgccatta acgctattcc ccgtgaataa catgcatgat gcgtagaata gcctcactat
    21301 agatgcctga ttcattgcat gtcatcaggc atcgactcga ttacacctct tcggcggcag
    21361 gcagcggcca gcctcccaga cgtttccagc ggttgacgat ttcacaaaac agcacagcag
    21421 tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct gtcgaagtcc atgccagcgg
    21481 tctggcaagc ctttgacaat acggtttgtc cgagtgccag cccggccagt gcagcagtgt
    21541 caaaagtggc gaaagggtgg aacgggttac gtttcagtga ggcgcgttct gcggcggcca
    21601 tcataaagct gtgatcgaaa ttggcgttgt gcgccaccat aatggcgcgg ttacagccgc
    21661 tcgctttaat acctttacgt acaactttaa aaatttcgtg cagcgcctcg tactcgctga
    21721 ccgcgccgcg atcgggatcg ttcggatcaa tgccattgaa ggcaagggcc tctggttgca
    21781 aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt ggtgtccggc atcagccagc
    21841 cttgttcatc cattttcagg gtgatggcgg caatctcaag cagcgcatcg gttttggcgt
    21901 taaatccggc tgtttcaaca tcgatcacaa caggataaaa accacgaaaa cggtcgcaca
    21961 gaccggtaag ttgagcgtta tcggacatct gattctctta atacggataa attgcagcgc
    22021 gcattatgac aaatattact tgtcgatgca gtaaagatgc gggcgcgatg agttcacgcc
    22081 cggcaggaga ttagttgccc agaccgcgac cggcgtcttt ctcttcgatt aattcaattt
    22141 tgtaaccgtc cggatcttcc acaaatgcga taaccgtagt accgcctttt accggacccg
    22201 cttcacgggt tacgttaccc ccgttttgac ggattttttc gcacgcttca gcggcgttat
    22261 ctacgctaag cgcgatgtga ccataagcag tgccgagttc gtatttatcc acgccccagt
    22321 tgtaggtcag ttcaatcact gcttcttcgg tttccgggcc gtaaccaaca aacgccagtg
    22381 agtatttgta ttccgggttt tcgctggtac gcagcagttt catgcccagc acgttggtat
    22441 aaaaatcgat ggagcgttgc agatcgccaa cgcgcagcat ggtatgaaga agacgcatct
    22501 ttatatcctc aaaatggcta acagattcaa aaagttgttt tagtatagcg gcggctttgc
    22561 gccgctcgca atgttggatt acagcgtcgg gtaatcagtg taaccttcag caccgccacc
    22621 ataaaaactt tcggcacgct gtgggttaag ctcagctttg cgctgcaagc gggcgaccag
    22681 atccgggttc gcaatccagt cacgaccaaa tgctaccgca tcaattaacc ctttgccgat
    22741 cagcgtttca gctttttcta ctgtgtatgc acctgcgccg ataatcggac cgtggaaacg
    22801 ggcgcgtact ttttcgcgaa acgcatcagt atatggttca cctcccgccc aatctggttc
    22861 tgacatatga agataagcaa tgccgcgttt acccagttgt tcaatcagat acagtgcatc
    22921 ggcttcttca ttcgggccgt tatccgtgtt ctggaaagta ccgattggcg aaatgcgaat
    22981 gccaatgcga tcggcacccc attcttcaat cccggcatcg accacttcca gcaccagacg
    23041 cgcgcgattt tccacgctgc cgccgtactg atcggtacga tggtttgaag aaggggaaag
    23101 gaactgatgc agcaaataac cgtgagcaga gtggagctct accagatcaa aaccggcttc
    23161 acgcgcgtta gcaatggcct gacggaaatc attgacgata cccggaatct ctcccagttc
    23221 aagcgcacgc ggcatggatg tttcaacacg gatcgcctga ccattttcat cgcgcagaga
    23281 agtacgtgtt cccgcgctaa gtgctgaagg cgctaccggt gcctgaccgc caggttgcag
    23341 gctggcgtga gaaatgcgtc cggtgtgcca cagctgtacg gccatatgac cattttcagc
    23401 atgaacgcca gcggtaattt ttttccatgc ggcaatttgt tccgggctat ggatgccagg
    23461 tgcacctgcg tatccttttg cctgggcaga aatttgcgtg gcttcgctaa taatcaaacc
    23521 ggcactggca cgttggcgat agtattccgc catcaacggg gtagggatgt cacccggttc
    23581 gatactgcgc agacgcgtca gcggtgccat aaaaatacga tttgccgccg tgatcgcgcc
    23641 cactttcagt ggggaataca gtttttcaga tgacataaag gctcccggtc gtctggtgat
    23701 aaaaaatgcc tgctaaacgg caggcgtcgc aataatgttt tttacatggg ccagcgcgtt
    23761 ttccagtggc tcgaaactgc gcgaaatttt ggcctgcaga ttcgcgccaa gccacagtgc
    23821 gtaaagcact tgtgcctgtt gcagcggttc gccacaaaag gttaaacaat ggttctcacg
    23881 gccattttcc agcgcctgag agagcagggc gatcacgccg cgagcccctt tatccattgc
    23941 gctgcgcata tcttctgaca gatcgcacac ttcggcagag agttttactg tcaggcaacc
    24001 actgatggtt ccatgttggc aaaactggtt cagtgtttgc tggtaataag ccagtatgcg
    24061 gtcgcggtag ttaccttcgc cggattgcag caactcagtc agtcgctggt gatatgcggc
    24121 gtaatggcgc tcaagcatag caacgccaaa cgcttcttta gaacgaaagt agtgatagaa
    24181 ggaccctttc ggcacttcag cggtttttag gagttcgctt agccccatcc cggtgaatcc
    24241 acgttgcagg caaagttgct cgcccgtcgc caggagatgt tcgcgagtat catgttcggt
    24301 gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc tagtgcaagc aaagttgcta
    24361 ctttggacat taacagcttc aataatggta ataatcttat ctgaggaggg cgaggtggcg
    24421 gagcaattag agttctttcc cgtccagagc ccgtgccggg gaatttgcca gtctgacgaa
    24481 cgcggatttt gtcgcggctg ttttcgtagc cgggatgagc gttttaactg gaataagatg
    24541 agcgatggcg aaaaacaaga agtgttgcgc ctttgtcggc agcgattaat gcgcaaatta
    24601 cgtgtaaata agtcagcgcc atcagacgaa cctgagcaac catcactctt ttgagcctaa
    24661 tcttgcgtat actcctctca tatctgataa gaggaagcaa ttatggttca gcgtattact
    24721 attgcgccgc aaggcccgga gttttcccgt tttgtgatgg gctactggcg attgatggac
    24781 tggaatatgt ccgcccgcca gttggtcagt tttattgaag agcatctgga tctaggcgtg
    24841 accaccgtgg accatgctga tatttatggt ggctatcagt gcgaagcggc gtttggtgag
    24901 gcgctgaaac tggcacctca cctgcgtgaa cggatggaaa tcgtcagtaa atgcggtatc
    24961 gcgacgaccg cgtgtgaaga aaacgtcatt ggccattaca tcactgaccg cgatcacatc
    25021 attaagagcg ccgaacagtc gctaattaat ctcgcgaccg atcatctgga tctgctgtta
    25081 atccaccgac cagacccgtt aatggatgcc gatgaagtgg cggacgcctt taaacatctg
    25141 catcagagcg gcaaagtgcg tcattttggt gtatcgaact ttacacctgc gcaatttgcg
    25201 ctgttgcaat cacgcctgcc atttaccctt gccactaatc aggtggaaat atccccggtg
    25261 catcagccgt tactgctgga tggcacgctc gaccaactgc aacaactgcg tgttcgtccg
    25321 atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg atgattattt ccagccgcta
    25381 cgtgatgaac tggctgtggt ggcagaggag ttaaacgcgg gctcgattga acaggtagtt
    25441 tacgcctggg tattacgttt accgtcgcag ccgctgccaa ttatcggctc aggtaaaatt
    25501 gagcgcgtac gggcagcagt cgaagcagaa acactgaaaa tgacccgtca acaatggttc
    25561 cgtatccgta aagcggcact aggttacgac gtaccgtaag cacttttagg aatagccgcc
    25621 gttcaaaaat gtgtcactgg tttacactta ttcaggaatg cacaatgaac ggaggtccta
    25681 tgaaacgttt tagtctggcc attctggcgc tggttgttgc gaccggcgca caagctgcca
    25741 gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt tgggcagtta attggtagcg
    25801 tcaccattac tgaaaccgat aaaggtctgg agttttcgcc cgatctgaaa gcattacctc
    25861 ccggtgaaca tggcttccat attcatgcca aaggaagctg ccagccagcc accaaagatg
    25921 gcaaagccag cgccgcagaa tccgcaggcg ggcatcttga tccacaaaat accggtaaac
    25981 atgaagggcc agaaggtgcc gggcatttag gcgatctgcc tgcactggta gtcaataatg
    26041 acggcaaggc gaccgatgcc gtcatcgcgc ctcgtctgaa atcactggat gaagtcaaag
    26101 acaaagcgct gatggtccac gttggcggcg ataatatgtc cgatcaaccc aaaccgctgg
    26161 gcggtggcgg tgaacgctat gcctgtggtg taattaagta attaagaagc cagcgtacct
    26221 tgcggcggtg cttgctcaag ttgcgaaagc gagcagtaca gccgccagac aattgccgcc
    26281 agttctcggg cggcaggttg atgatgacgg gcaaggctgt cgcaaattcg ctgtaattct
    26341 tcaagtgtgg ccgccagtga tttttgctga acgccacgct cactcatcac tccccgcaac
    26401 agtgaaatac agttatcccg tactcgcgat aacggatcgg aacgcgattc ccagtcgcgc
    26461 aattgccaga caacatgaga acagttcagc aacactacac cccagcgtaa taaccaacgg
    26521 cgagccagcg catcctggct gttactcaac tgactgacgt gatgataagt gagcgattca
    26581 aattcgcttt cactcagtgt tggatggcgg cttagttgat cgacaaaatc ccggcgtagc
    26641 gcgcgaatat ggcggcggct tttacgagca tccgatcctg gacgcagaat ggcgaacgct
    26701 aaccacgcca acgcgacgcc aacaattttt gccagattat cgttaagaaa atcagcaaaa
    26761 tcatacaccg ggggattagt gacagcgata aacgaaccca taaaaacaat cagttgcccc
    26821 cacagtgcgg caaatttggg catctgcaat ttaagaagct gcattgtcgc cagcagtgga
    26881 aagagaaaca gtaaaaattg ccacagatcg ctaatctgca ccatcaggcc aaatttgacc
    26941 acaaagctga atagcgaaag taacaccagc gtgcgcatca gcagtgacaa cgacttaaac
    27001 ggtgccgcga cggcggagta gagtacgcag ctaattgctg ccagcgttaa agcattggca
    27061 ccggcatccc attgcgaagc aatactccat gcgccaatca tcatcaacgt acaaaatgta
    27121 cgcagcccgc tccacatagc ttcggcgtta tcggtatgac gagccaggcc ggatgtccgt
    27181 gggagtctgg catgatcatc tacaccgctt tgcagacgat gtaattcctg actactttgc
    27241 agataaaggc ggcaaaaata acgtagtcgc tgccagaagg cgatgtgccg gtagtcggcg
    27301 acgttggtcg ggcgtagtgg ggagataata cgtgcgatgg tgtaaacatc tgtttgcgaa
    27361 ctggcgagcg ccgtcagcag ctgttcgaga atttctcgcg tggcatctgg cggtgagggc
    27421 cagttgagca acatacgtcg caggctggag atgacactgg tcatacgtaa ttgctggtgg
    27481 agtagcgcat taaggcgtgc gttttgctgg cgaaaacgat agtggctcca gaaagcctgg
    27541 atacgcagca aattcatggt cagtatctgc ccaatcaccc cttcatgtgc agcacgaatg
    27601 gcatcggttg tttcaggccg ccagagtaaa ctggcatgtt ccagtaatcg ggcgtgcatg
    27661 tttttcaatg cggttaaaag ggctgtagca tcggaactgc tcggcaggat catcatcatc
    27721 atgccgccgc acaaaatacc gactatcacc tcgcaaacac gcgcctgagc gatatcccac
    27781 agctggctgg cctcagtaat attaaccatc ggaaaggcga tgatggcagc cgtgtagccc
    27841 gccagttgaa atgcatacgc gacgttattc gtgaagtgcg cacaggccca ggtacaaaag
    27901 ccaagccacg ccgacatgct caatagaaaa aaccacggct cattgagcgt atgtccggca
    27961 agaagcagtg ccgcaatggc tccgagcaaa ctgccagcga tgcgtccgag gcttttgctg
    28021 atgacaccgc caacggtggg aaagctaacc accgcagccg aggtcatcgc ccaatagggc
    28081 tcatcaagat tcaaataata ggcaaccgtc agcgccagac acatggcaat ggtattgcgt
    28141 aacgcataac gccattgcgc cagcgtggcc ctgaaccagg gcaaattgcg caaggaccat
    28201 gacgatgcgt tcattattga cctacggcaa tgctacaggt ggtgcccgac accagcgtga
    28261 catcgcctgg gactttatcc agcgtaaagc ggacgggaac gcgttgggcg aggcgaaccc
    28321 aggggacgtt aggtttcaca tccggaataa ggcttgagtc actttcaacg ctctgatcat
    28381 aaatcgcccg accgatactg gacacgtgac cctgtaacgt tttgttatca ctatagaggg
    28441 taatttgtgc tggtgcacct tcgcggatat ggcgcaactt ggtttcttca aaataaccaa
    28501 tgacgtaaaa cgagtggctg tcaacaaggg caaacagcgg tttcccggtg tcagcgtagt
    28561 cgccgatgcg ggtagtgaga ttagtcaccc atccactcac cggggcgcga atttccgttt
    28621 gcgccagttg ccattgtgcc tgtttcagcg tcgcctgtgc ggcatcaacg ctggcctgca
    28681 tcgctttaac attgaggtta gcggtatcga gttcttcggc agaaataaaa ttttgtgaga
    28741 gatgacgacg acgattcgct tcgttattgg cttttgcgag gtcagattgc gctttcgcca
    28801 gctgagcctg tgcgttcagc tcggcgatct gaaagggcgt tttatcgatc gtgagcagaa
    28861 gatcccccgc gttaaccagc tggttgtctt ttatattcag ctcaacaata cgtcccgaca
    28921 cctgtggggt gatagaaacc tgttctgcgc gtattttccc gtcgcgtgtc cacggagatt
    28981 gcatgtaata attccacagc caccatccgg caagaaccgc aactaccgct acaatgattg
    29041 ttgagaaata cttaattgtt ttaattgaca tagttaccac gcaatcagta ttgccagagc
    29101 aaggcaaacg caaatcgcaa acagcgataa atccattaac aagggatgcc agatgtcacc
    29161 ggcgtagatc cagcctcgaa gcaggcggtg tatgacaagc cagatgacga atccaaatgc
    29221 gaaggctttg aaaaacggag gaaagtagac ggacgcaccg aacaccagat cttgtaaggg
    29281 caatcctgtt gcattgagca taaacttcac aaagtagatt cctttacgac agaatatatt
    29341 gcgttagatt aataaatatt ctttaagtgc gaaaaattta cgcgcaattt ctgaaaaata
    29401 tgtctgacgg taaccaaatg cagcaataca tttgttttag caatacaatt gctgcacact
    29461 attctaaaag ccgcataata tcttagcaag ctaattataa ggagatgaaa ttggaatcgc
    29521 cactaggttc tgatctggca cggttggtgc gcatatggcg tgctctgata gaccatcgcc
    29581 tgaaaccgct ggagttaaca caaacccatt gggttacgtt acacaatatc catcagttac
    29641 ctccagacca gtcgcaaatt caactggcaa aagcgattgg cattgagcag ccatcactgg
    29701 tccgtacgct ggaccaactg gaagaaaaag ggttaatttc gcgtcaaact tgtgccagcg
    29761 atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga gccactgatc agcgaaatgg
    29821 aagccgttat taacaaaacc cgcgcggaaa ttttacatgg catctccgca gaggaactgg
    29881 aacaactgat taagctcatc gcaaaacttg agcataatat cattgagtta caggccaaag
    29941 ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca cgcaaactta tttagcgcgg
    30001 ggaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcctg gagagaaatg
    30061 agtgttccct tgtttctgta ccaccatgat ggtgttgcca tcatctttac gaatttccag
    30121 ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc tgaccggcta caccacctgc
    30181 aacagcccct gctgcagtag ccagagaacg cccggttccg ccgccaatag tatttcccag
    30241 gaaaccacca agtacagcac caccaattgc gccgataacg ttggaatcat caccgccctg
    30301 aatctgtacc tgacgtacat taacgatggt gccatagctg acattctgta cttgtttcgc
    30361 ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac cgacaagaga
    30421 cagacctacc attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc ctgttcacca
    30481 tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga atccatggtt
    30541 acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa ttataaacaa
    30601 aatattcatg atgattattg atttgaagat aatgttttac taccaacggt aaaaattggt
    30661 cattccgcat ggtattcacg aaaagtttat gtttgagtgg tgcgcaaacg tcagattgaa
    30721 aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac cagccttgat
    30781 ggtgttgatg ttgtgttggc gacaattgat gaacaccggg tcgcacagct ggcaagtttg
    30841 agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca gggccagcag
    30901 cttacacttt cgcagtttgg acagcttgat actcaacttg ggcgactttt tgctgatgca
    30961 gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatagttgc gatcggctgt
    31021 cacggtcaaa ccgtctggca cgaaccgacg ggcgtggcac cacacacttt acagattggc
    31081 gataacaatc aaattgtggc gcgcaccgga attacggttg tcggtgattt tcgccgtcgc
    31141 gatattgcct tgggaggaca aggtgcaccg ctggtacctg cgttccatca tgcgctgctg
    31201 gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgccaa tctgtcactg
    31261 ctcattcctg gccagccggt tgggggctac gataccggtc ctggtaacat gctgatggat
    31321 gcctggatct ggcgtcaggc cggtaaacct tacgataaag atgccgagtg ggcacgggcg
    31381 ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccatattt ctcgcaaccc
    31441 gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg ccatttgcgc
    31501 cattttccgg gggttgatcc ccgtgatgtg caggccacag tggcagaact caccgccgtg
    31561 accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt atgtggtgga
    31621 ggtagtcgta atccgctact catggcgcgt ctggcggcat tactgccagg cacagaagtc
    31681 accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc gttcgcctgg
    31741 cttgcctggc gaacgctggc gggattacca ggaaatctgc cttccgtcac tggcgcaagc
    31801 caggagacgg tactgggggc tattttcccc gctaacccgt gatatcagag ttaactgaat
    31861 ttttcttacc gcgtctgccg ttacacttgg aacgttaggg agggcgtatt gccctccaga
    31921 ccaggaaagt cttcgggata tgtctatgaa aaaactgtta ctgatctgtt tgccggtgct
    31981 tctcaccggc tgtagctcgt tcaatcaact ggttgagcgc atgcaaaccg atacgctgga
    32041 ataccagtgt gatgaaaaac cgttgacggt caaactgaat aatccgcgcc aggaagtcag
    32101 ttttgtttac gataatcaac tactgcatct caaacagggc atttcagcgt ctggtgcacg
    32161 ttacaccgac ggaatctatg ttttctggtc gaaaggcgag gaagcgactg tctataaacg
    32221 cgaccgcatc gtcttgaata actgtcagtt acaaaatccg cagcgttgag atttttccag
    32281 gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg tctgataacg
    32341 acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcggg ttacgccgcc
    32401 gcgatctacc cgccgatcca ttaacccttt ttgaacgttg gctctctcag gcttgtgaag
    32461 ccaaactggc tgaccctacc gcgatggtgg tcgctaccgt ggatgaacat ggtcagcctt
    32521 atcagcgcat cgttttactc aaacattacg acgaaaaagg catggtgttt tacaccaacc
    32581 tcggcagccg taaagcacat caaatcgaaa ataatccgcg cgttagcctg ctgttcccgt
    32641 ggcataccct tgaacgccag gtgatggtga tcggtaaagc ggaacgactt tcgactctcg
    32701 aagtgatgaa atattttcat agccgcccgc gtgatagcca gattggtgca tgggtttcga
    32761 agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg gagctgaagc
    32821 agaagtttca acagggcgaa gtgccattgc caagtttttg gggcggtttt cgcgtcagcc
    32881 ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc tttttgtacc
    32941 agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat gcaaaaatct
    33001 tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt tcgcatctgg
    33061 cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt tgatggcaag
    33121 cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga cggacgagga
    33181 agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct tcgatcctac
    33241 cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac gcttccagca
    33301 ggcgggccac aagccggttg cgctggtagg cggcgcgacg ggtctgattg gcgacccgag
    33361 cttcaaagct gccgagcgta agctgaacac tgaagaaact gttcaggagt gggtggacaa
    33421 aatccgcaag caggttgccc cgttcctcga tttcgactgt ggagaaaact ctgctatcgc
    33481 ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc gcgatattgg
    33541 caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc gtctcaaccg
    33601 tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg gttatgactt
    33661 cgcctgtctg aacaaacagt acggtgtggt gctgcaaatc ggtggttctg accagtgggg
    33721 taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg tgtttggctt
    33781 gaccgttccg ctgatcacca aagcagacgg caccaaattt ggtaaaactg aaggcggcgc
    33841 agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct ggatcaacac
    33901 tgcggatgcc gacgtttacc gcttcatgaa gttcttcacc ttcatgagca ttgaagagat
    33961 caacgccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc agtatgtgct
    34021 tgcagaacag gtgacacgtc tggtgcatgg tgaagacggt ttgcaggcgg caaaacgtat
    34081 taccgaatgc ctgttcagcg gttccttgag tgcgctgagt gaagcggact tcgaacagct
    34141 ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc gcagacctga tgcaggcgct
    34201 ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg cctccaatgc
    34261 catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag aagaagatcg
    34321 tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc tgatttgctg
    34381 gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt ttttatacag
    34441 ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt catgcgggta
    34501 acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg ctgaacaccg
    34561 ttcaattttc taatcacacc caatacggca aatggaccgg ttgcgtgatg ccgcccagcc
    34621 atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc tgtgatgccg
    34681 tattaagtgg ctatctggga tcggcggagc agggtgaaca tatcctcggt atcgttcgtc
    34741 aggtgaaagc agcgaatcca caggcgaaat atttttgcga tccggtaatg ggtcatccgg
    34801 aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac ggtttgcctg
    34861 ccagcgatat cattgcgccg aatctggttg agctggaaat actctgtgag catccggtaa
    34921 agaacgtcga agaagcggtt ctggcagcgc gcgaactcat tgcgcaaggg ccacaaattg
    34981 tgttggttaa acacctggcg cgagctggct acagccgtga ccgttttgaa atgctgctag
    35041 tcaccgccga tgaagcctgg catatcagcc gtccgctggt ggattttggt atgcgccagc
    35101 cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt cagggggcaa
    35161 cgctgcagga ggccctggaa catgtgaccg ctgcagtata cgaaatcatg gtgaccacaa
    35221 aagcaatgca ggaatatgag ctgcaagtgg ttgctgctca ggatcgtatt gccaaaccag
    35281 aacattattt cagcgcaaca aagctctgaa atatctcatt tcggcccaca acgacctgtg
    35341 ggccgatggc tattacttta agccttccgc tgacagcgcg tcttgtactt ccggacgttc
    35401 agccatacgt tgcataaatg ccgcaatgtg ctctaaccct tccagattca gtttcaccgc
    35461 gtatgcccag cgcagaaccg taaacagata ggcatcagca attgtaaatc gttgcccgca
    35521 gatccagtgc tcatccttca gtgcctcgtt cacatattgc agcttcttct ccagctgcgc
    35581 gcgaactgtt ggtttgtact cttccggtgt atcagggcga aacagaggtg tgaagccttt
    35641 atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg aaatactgtt
    35701 taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca taatcgctac
    35761 gccttccgtc agcaaagtac catcgtccag cagcaatgca ggtacctgtc ccttagggtt
    35821 aacggcaaag taatcgtcac cgttttcgag acgttttttc attaaatcca cactgacgag
    35881 cgtaaaatcc tttccgctct cacgcagggt tatatgggaa gcgagagagc aggcgcccgg
    35941 tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat ggtagtgcgt
    36001 tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga gtttccctga
    36061 attttacgct acggctgctt tcgccgcttt gtctgcagcg tcatcctgcg tcatgcggtg
    36121 cagtttcggc gcggtaaaca gcatcagtac tgcaataacg gcagtagcga caccaatctg
    36181 caagaatacg cgaccataga cttccagtga catcagcgga tcggtaacgt tatccggcac
    36241 agccatcata cctgcaacat aaccaccaat caggtttgca ccggcagtgg tcaggaacca
    36301 gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca ttgccagacc
    36361 caggccagag atcatcagtt ccccgatact ctgcaggcca tagctcgcga ccagccagct
    36421 tacagacacg ataccagcgt cagacgcgaa tttcgcaccc agcggcagaa tcaggaacgc
    36481 accagaacac atcaccatgc cgatagcaaa cttggttggc attggcaggg tatcgcccat
    36541 cttgttatag atagcggcca gaatcgggct accaatgatg atccagaacg ggttcagtgc
    36601 ctgatactgc tcaggttcta cggccaggcc cagaatggtg tgttcaacgt tacgaatcgc
    36661 aaagaagttc agtgacgttg gcatctggct ataaagcacg aagaagataa tggcttcgag
    36721 catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg cgaaggcttc
    36781 tttaccgaag ataaccacga taccgaaggc aacaacgccc agcgccatac gcgcaacttc
    36841 ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa taatggtcag
    36901 cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact gtttaaccca
    36961 acgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc tcaacgcaaa
    37021 cgcaacactc cagccgtatt tcgcggccag ccacggcgta gcaatcatag agaagaaaga
    37081 gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgcg ggtcgttttt
    37141 ctcatagcat gtagaaagca gagaagacgg gttagcttta aacaggccgt taccgaccgc
    37201 aatagccgcc atacccatat aaacgatacc ggcgtcgtga ccagaccagg caaccagagc
    37261 ataaccaatc gccagcacaa tagcgccgag cataattacg cgtttagtac ccagtacctt
    37321 gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa aggaagagaa
    37381 aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa cagccataat
    37441 tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga tgagatagaa
    37501 cgccttcggt tgtttaaaag cgttcaaact gacgctttca gttggttttt ggtttgcagt
    37561 ggacactttt acctcttttt ttacatccca tattaacggg ggtgctttct cgcgttatgt
    37621 ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa tgttcactat
    37681 cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag gcggggaatt
    37741 caaccattcc gggaaatgtt atccagcgtt aaatttttca aaaaacgtaa cggcagattt
    37801 tttcactacc ctgagagggt gatgatggct taatggcgat atgttctact atttggcgtt
    37861 tttcctagtg agatagtcca gtttctgaaa aatagccagt gtaatgtttt gtaggtcaat
    37921 atttgttgcg tgcgtaaatt taaatgattt taacctgatt cttgcaatgt ttttacattg
    37981 ccacagcatc gtgatcttga tcacgaatga atagaaaatt tcgctattaa aaaaacaatt
    38041 aacctgcatt tttggttatc tgcaaacgga ttacctgatg ataacgctaa gcaatggcat
    38101 cattgacgtt aaaaagatgt tttaaaagag gataaagaaa ggttatgaat ggggtaatcg
    38161 gtgttacccc tgatctttag atgtcaactt tctctttgta ttcacaaaga tcttcaataa
    38221 tacaagagcc acagcgaggc ttgcgggcaa tgcaggtata acgcccgtgc agaatcaacc
    38281 aatggtggca gtcgacttta aactctgccg gaaccacttt cagtagcttt tcttctacct
    38341 gttcgacgtt tttccccggc gcaaattgag tacgattgca aacgcggaaa atgtgcgtgt
    38401 cgacagcaat agtcggccag ccgaatgcag tgtttaatac gacgttggct gttttacgac
    38461 ctacgccagg cagggcttca agcgcagcac gatcttccgg aacctcgcca ttatgttgtt
    38521 ccagtaatat acggcaggtt ttgatgatat tttctgcttt gctgttataa aggccaatcg
    38581 ttttgatata ggttttcacc ccttcaacgc ccagttcaag catcgctgca ggcgtattag
    38641 ccaccgggta gagtttcgcc gtcgccttat taacactgac atcggtcgcc tgagcggaaa
    38701 gcagtacggc aatcagcaat tcaaaaggcg aactgaaatt aagctcggtg gtgggatgag
    38761 gattgttctc acgcaggcga gtgaggatct ctaggcgttt tgctttgttc atcagacatt
    38821 ccctgtttca ccgtttggca atgcacgttc ggcaactgct tcagtacggc gctttttcat
    38881 tttttcatca atcaggtatt ttcctgccag catcagtccc aggccaataa atgcacctgg
    38941 tggcagcatc gccagcagga aaggggagtc ggtgtggaaa atctccacgc gtaatacttt
    39001 cgcccagcta cctaacagcg catctgcacc gtcaaacaac gtgccattgc cgataatttc
    39061 gcgtagtgaa cccagcacga acatggcgca ggttgcgccc ataccaattg aaaagccgtc
    39121 cagtgccgaa agcgccggac cttttttggc ggcgaaggct tcagcgcggc ccacaacgat
    39181 acagttagtg acaatcagcg gaataaaaat ccctaatgat tgatacaggc caaaggcgta
    39241 ggcgttgatc agcatctgta cagcgctgac caccgaggcg atgatcatca cgtaaatggg
    39301 aatgcggatc tcggcgggcg tccagtgacg cagcgtcgaa atggtcaggt tggttagcgt
    39361 cagtaccagc gtagtcgcaa gtcctaaacc cagagcgtta gtggcagtgg acgtgaccgc
    39421 caacagcgga caaaggccga gcaactggac cagcgcagag ttgtttttcc acaacccctg
    39481 aacaataacg tctttaattt cgctcacgtt ttattctcca caggcaggaa gttgagaaag
    39541 ttgtgccggt aacgtctgag cgtacaatcc ggcgcgtttt accgcattaa ccaccgcgcg
    39601 gggagtaatc gtcgcgccgg tgaactggtc gaaatcacca ccatctttct tcaccgccca
    39661 gttcgcatca tccgcaccac tgattttttt acccgcaaaa tgggtgatcc agtcagaaag
    39721 gcgcagttcg attttatcgc caagccctgg cgtttcgtgg tgctcagtca cgcgcgtgcc
    39781 aagtaccgtg ccgttaaaat cggctcccac cagcagctga atcgcaccgg aatagccatc
    39841 tggcgcggtt gcttccagaa cggcagctac cggtttgtca tcctgtttgg cgatgtaaac
    39901 ccgatgctca cctttaccta actctggcgc agttaccaga tagcaactct gtgccagcgc
    39961 attgttatag cgttcagctg gcagcacctg atcaaataac gccttttgtt gcagactggc
    40021 ctgttcagca attgtcgttt tggtcatctg gttgatggcc gcagttaacc ctgttgaacc
    40081 cgctgcaaag agcgccaacg taatgccgtg ttttcggata gttttcagca tggtttaccc
    40141 tttgcgatgg ccgtagacgc gcggacgcgt gtagtaatcg atcagaggaa ccgtgatgtt
    40201 cgccagcagg acggcaaaag caacgccgtc aggatagccg ccgaaactgc ggatcatcca
    40261 gactaataag cccgccagcg cgccgaaaat aagacgacca cgattggtcg tagaagcggt
    40321 aaccgggtca gtcaaaataa agaatgcgcc gagcatggtc gcaccagaca gcagatgaat
    40381 ttgcggtgct gccagagtat ctggtgagaa caaccagccc aacgttgcgc ataacgccag
    40441 cgttactaag aagctgaggg gaacatgcca gcgaatcgct ttctgccata gcaaccacac
    40501 gccgccagcc agccaggcga gatttaccca ttgccaacca gcgcccgcca gaataccgct
    40561 gtagatcgga tattgcataa tctcttcaac cgaatgaccg gcacggacag aggttttaaa
    40621 tgtatccagc ggtgtcgcct gactaatgcc atcaatacct aagcgtagcg tgttcatatc
    40681 accaccactg gtggtatgac cactgaaaat aacctgtatg gcgtcgataa aaccagggat
    40741 gttgaccgca atttcatgtg gcggtaacca actggtcatc tgcaccggga aggagatcag
    40801 taagaccaca taaccaatca ttgccggatt aaacgggttt tgccccagac cgccatacaa
    40861 ctgtttagcg atgatcaccg caaacaccgt acccagcacg accatccacc atggcgcgag
    40921 agggggaata cttaccgcca gcaataagcc tgtcagcaat gctgagttat ctttcaacgt
    40981 tgcagctacc gactgcttgc gtagtttgag gacgagagct tcggccagaa gagcactgac
    41041 cgacgccaac aggatctgaa cgagagtacc ccaaccaaaa aaccacagtt gcgctgcgat
    41101 tcctggcacg gctgcgagca ataccaacag cataatgcgc gatgtctggc gctggttatg
    41161 ggtataaggg gagctagcta ttctgaatac catttagtcc tcgtttacaa ccttttgctg
    41221 ggcggctttt ttggcctgaa cgcgggcaat agccgcagca actgccgctt tgcgcggatc
    41281 aatctgttct tctggctctg cattagcctg ttgctgttcc agcttgcgtg ctttggcacg
    41341 ggcaatagcc gcttcgacgg c
//
LOCUS       DADTFG010000022        41103 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.022, whole genome shotgun sequence.
ACCESSION   DADTFG010000022 DADTFG010000000
VERSION     DADTFG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.022"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(97..657)
                     /gene="nudE"
                     /locus_tag="J1138_001144"
     CDS             complement(97..657)
                     /gene="nudE"
                     /locus_tag="J1138_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBA8452974.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            977..3112
                     /gene="igaA"
                     /locus_tag="J1138_001145"
     CDS             977..3112
                     /gene="igaA"
                     /locus_tag="J1138_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBA8452975.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFMMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            3177..3845
                     /gene="yrfG"
                     /locus_tag="J1138_001146"
     CDS             3177..3845
                     /gene="yrfG"
                     /locus_tag="J1138_001146"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBA8452976.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            3856..4257
                     /gene="hslR"
                     /locus_tag="J1138_001147"
     CDS             3856..4257
                     /gene="hslR"
                     /locus_tag="J1138_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBA8452977.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            4282..5160
                     /gene="hslO"
                     /locus_tag="J1138_001148"
     CDS             4282..5160
                     /gene="hslO"
                     /locus_tag="J1138_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBA8452978.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(5223..6947)
                     /locus_tag="J1138_001149"
     CDS             complement(5223..6947)
                     /locus_tag="J1138_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBA8452979.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNHWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNLLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDGFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTADRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLVVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIGKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSTRKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLVVDGE
                     RLDVNLNE"
     gene            7326..8948
                     /gene="pckA"
                     /locus_tag="J1138_001150"
     CDS             7326..8948
                     /gene="pckA"
                     /locus_tag="J1138_001150"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBA8452980.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(9025..10377)
                     /gene="envZ"
                     /locus_tag="J1138_001151"
     CDS             complement(9025..10377)
                     /gene="envZ"
                     /locus_tag="J1138_001151"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBA8452981.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGMTKEG
                     "
     gene            complement(10374..11093)
                     /gene="ompR"
                     /locus_tag="J1138_001152"
     CDS             complement(10374..11093)
                     /gene="ompR"
                     /locus_tag="J1138_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBA8452982.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            11285..11797
                     /gene="greB"
                     /locus_tag="J1138_001153"
     CDS             11285..11797
                     /gene="greB"
                     /locus_tag="J1138_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBA8452983.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            11894..14215
                     /locus_tag="J1138_001154"
     CDS             11894..14215
                     /locus_tag="J1138_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBA8452984.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            14653..14880
                     /gene="feoA"
                     /locus_tag="J1138_001155"
     CDS             14653..14880
                     /gene="feoA"
                     /locus_tag="J1138_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBA8452985.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            14897..17218
                     /gene="feoB"
                     /locus_tag="J1138_001156"
     CDS             14897..17218
                     /gene="feoB"
                     /locus_tag="J1138_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBA8452986.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDVELLATRKSVSSCCAASTTGDCH"
     gene            17218..17454
                     /gene="feoC"
                     /locus_tag="J1138_001157"
     CDS             17218..17454
                     /gene="feoC"
                     /locus_tag="J1138_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBA8452987.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGGCKSCPEGKACLREWWALR"
     gene            17687..18280
                     /locus_tag="J1138_001158"
                     /pseudo
     CDS             17687..18280
                     /locus_tag="J1138_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(18309..19079)
                     /gene="bioH"
                     /locus_tag="J1138_001159"
     CDS             complement(18309..19079)
                     /gene="bioH"
                     /locus_tag="J1138_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBA8452988.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            19117..19800
                     /gene="gntX"
                     /locus_tag="J1138_001160"
     CDS             19117..19800
                     /gene="gntX"
                     /locus_tag="J1138_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBA8452989.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            19859..20434
                     /gene="nfuA"
                     /locus_tag="J1138_001161"
     CDS             19859..20434
                     /gene="nfuA"
                     /locus_tag="J1138_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBA8452990.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            20798..22114
                     /gene="gntT"
                     /locus_tag="J1138_001162"
     CDS             20798..22114
                     /gene="gntT"
                     /locus_tag="J1138_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBA8452991.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(22225..24309)
                     /gene="malQ"
                     /locus_tag="J1138_001163"
     CDS             complement(22225..24309)
                     /gene="malQ"
                     /locus_tag="J1138_001163"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBA8452992.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVMGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESSSPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(24319..26712)
                     /gene="malP"
                     /locus_tag="J1138_001164"
     CDS             complement(24319..26712)
                     /gene="malP"
                     /locus_tag="J1138_001164"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBA8452993.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            27335..30040
                     /gene="malT"
                     /locus_tag="J1138_001165"
     CDS             27335..30040
                     /gene="malT"
                     /locus_tag="J1138_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBA8452994.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICDTMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVMYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(30083..31099)
                     /gene="rtcA"
                     /locus_tag="J1138_001166"
     CDS             complement(30083..31099)
                     /gene="rtcA"
                     /locus_tag="J1138_001166"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBA8452995.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLQTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(31103..32329)
                     /locus_tag="J1138_001167"
     CDS             complement(31103..32329)
                     /locus_tag="J1138_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HBA8452996.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVMQKTVKQPQTLAMEE
                     INCHHNYVQKELHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            32518..34116
                     /locus_tag="J1138_001168"
                     /pseudo
     CDS             32518..34116
                     /locus_tag="J1138_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
     gene            complement(34098..34856)
                     /locus_tag="J1138_001169"
     CDS             complement(34098..34856)
                     /locus_tag="J1138_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBA8452997.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(34873..35703)
                     /gene="glpG"
                     /locus_tag="J1138_001170"
     CDS             complement(34873..35703)
                     /gene="glpG"
                     /locus_tag="J1138_001170"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBA8452998.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(35748..36074)
                     /gene="glpE"
                     /locus_tag="J1138_001171"
     CDS             complement(35748..36074)
                     /gene="glpE"
                     /locus_tag="J1138_001171"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBA8452999.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            36264..37769
                     /gene="glpD"
                     /locus_tag="J1138_001172"
     CDS             36264..37769
                     /gene="glpD"
                     /locus_tag="J1138_001172"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBA8453000.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLNRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDEDGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS"
     gene            complement(37823..38428)
                     /locus_tag="J1138_001173"
     CDS             complement(37823..38428)
                     /locus_tag="J1138_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453001.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(38431..39198)
                     /locus_tag="J1138_001174"
     CDS             complement(38431..39198)
                     /locus_tag="J1138_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453002.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPKQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITAQINAWAFNNQSILKERARQAIEN
                     PLKQPEQGTQYQIWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(39201..39911)
                     /locus_tag="J1138_001175"
     CDS             complement(39201..39911)
                     /locus_tag="J1138_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453003.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIVQQLINQV
                     LDEGEKFAGAAGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDLY
                     ISDVPDVQCDLNTTNDFTVQFTAMLNPDERVIIAKRPLKKCDAWIEDVYIFQVFKEAW
                     QFQNDNSLRGFRDKQAELKLYARHYSVENCTEESCWDCNYCIRPSFSLSRSAIIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(40085..41035)
                     /locus_tag="J1138_001176"
     CDS             complement(40085..41035)
                     /locus_tag="J1138_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8453004.1"
                     /translation="MVSDVNKNAPGTVLPSQIWKVGTYKAYCNSLDDYEIYFSAVSGI
                     DPSGASGDHQGSDVFIPLTHEISVSTHIKLYNQNGTMTDKIVPFENYNTNYPGDRSKP
                     SNWASGTEGYIKIRIDKKIISDVSLSNVLLVSLYVSQIPTEHGPIPVFNAYIGNLNIQ
                     VPQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMT
                     LVFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVEN
                     QAASAQTADVTFLTLPVSTTGKVPAAGRYNALAILRVEYQ"
BASE COUNT        10047 a        11018 c        10278 g         9760 t
ORIGIN      
        1 aacgcgttat ccggccaaca aaaaattgaa aattcaataa attgcaggaa cttcgtaagc
       61 ctgataagca ttatgcatca ggcaaacttc acgcatttac actcgcccct gccctttcaa
      121 ccattcgcgc acgaggaaca gcgcgctgac attacgcgct tcgttgaagt caggggcttc
      181 cagcaaatcc atcatatgcg ccagcggcca gcgcacctgt ggtaacggct ctggctcatc
      241 gccttccagt gattccgggt agagatcttg cgctaccacg atattcattt tgctggaaaa
      301 gtaagacggt gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac
      361 ctcttctttt agctcgcggt tagcggcttc aaagacgctt tcacccggat caattaatcc
      421 tttcgaaaaa cctaattcgt aggattcagt tcccactgcg tattcgcgga tcaggatcag
      481 gtgatcgtcc acaatcggca caatcatcac tgcttcccgg ttggttggac gcattcgttc
      541 ataaacacgc cgcacgccat tgctgaactc cagatccacg ctttcgacgg taaacagtcg
      601 ggaacgggct acagtttcaa cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt
      661 gggatctatg ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt tcggcaatgt
      721 gaattgcgtg ttatttacat ttatgtaact taataaataa tcgtcctcaa atcaaattaa
      781 aagtcaatag gttgaaataa ctccaggaat ttgctgatat tccgccttca gagggtttgc
      841 tatgatcagc ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg
      901 atagccaacc gcagaatcat gtattgtgtc cggtgcgact gaccacgcct gacagactaa
      961 gtaagatggg gaaagcatga gcaccattgt gattttttta gctgctttgc tggcctgctc
     1021 actacttgcg ggatggctga taaaagtgcg atccagacgg cgtcagctgc cctggaccaa
     1081 cgccttcgcg gatgcgcaaa cgcgtaaact cacatctgaa gaacgtagcg ccgttgaaaa
     1141 ttatcttgag agcctgacgc aggtattgca ggtgcctggc ccaacgggag ccagcgcggc
     1201 accgatctct ctggcgctga atgccgaaag caacaacgtc atgatgctga cacacgctat
     1261 cacgcgttat ggcatctcta ccgacgatcc gaataaatgg cgttactacc tcgattcggt
     1321 agaagtccac ctgccccctt tctgggaaca gtacatcaac gatgagaata ccgttgaact
     1381 gattcatacc gattcgctgc cgctggttat ttcactcaac ggtcatacgc tgcaggagta
     1441 catgcaggaa actcgcggct atgccttgca acctgttccg tcaacgcagg cgtcgattcg
     1501 cggggaagag agcgagcaaa tagagctgct caatattcgc aaagaaacgc acgaagagta
     1561 tgcgctgagt cgtccgcgcg ggctgcgcga agcgttgctg attgtcgcct ccttcatgat
     1621 gttctttttc tgcctgatta ccccggatgt ctttgttccg tggctggcag gcggcgcgtt
     1681 actgctgctg ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat cctccctgcg
     1741 ggaaattcat tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg aaaacgatca
     1801 ggaacagatc aacaatattt cactcggtat tatcgacctg gtctatcccg cacactggca
     1861 gccatacatt gctcaggatc ttggtcaaca aaccgatatc gatatctatc tcgaccgcca
     1921 cgtagtgcgt caagggcgat atctttcgct gcatgatgaa gtaaaaaact ttccgttaca
     1981 gcactggctg cgcagtacga ttattgctgc gggctcgcta ttggtgctgt ttatgctgtt
     2041 attctggatc ccgctggata tgccgctgaa attcaccctc tcgtggatga aaggcgcgca
     2101 gaccattgaa gccaccagcg tgaaacaact ggctgacgct ggcgtgcggg ttggcgatac
     2161 attgcgtatt agcggtacgg gaatgtgtaa tattcgcact tccggtacct ggagcgcgaa
     2221 aaccaactca ccttttttac cgtttgactg ctcgcagatc atctggaatg acgcccgctc
     2281 attgccatta ccagaatctg aattggtcaa caaagcgacg gcattgaccg aagcagttaa
     2341 tcgccagctg caccctaaac cggaagatga atctcgcgtc agtgcctcat tacgttcagc
     2401 aattcaaaaa tccggtatgg tattgcttga tgattttggc gacattgttc tgaagacagc
     2461 agatctgtgt tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg
     2521 caacagtaaa gactgggacg cgctggtaaa acgcgccaac gccgggaaac tcgatggcgt
     2581 gaatgtgtta ttacgcccgg tgagtgcgga atcgctggat aacctggtgg ccacctctac
     2641 cgcgccgttt atcacgcatg aaacggcgcg agcggcacaa tcactaaaca gtccggcccc
     2701 cggcggattc ctgattgtca gcgacgaagg cagcgatttt gttgatcagc cctggccttc
     2761 ggcatcactt tacgactatc cgccgcaaga acagtggaac gctttccaga aactggcaca
     2821 aatgctgatg catacgccgt ttaacgccga aggtatcgtc acaaaaatct tcactgacgc
     2881 caatggtacg cagcatattg gccttcatcc gatcccggat cgttccggcc tgtggcgcta
     2941 tctcagcacc acgctgctgc tactgacgat gctgggtagc gccatttata atggcgtaca
     3001 ggcctggcgt cgttaccagc gccatcgtac tcgcatgatg aagattcagg cctattatga
     3061 aagctgcctg aacccgcaac tgatcacccc ttcagaaagc cttatcgaat aacacgtttg
     3121 cgcggcaggt tatgctaccc tgtcgcgcaa attgcttcac tctggagatt tccctcatgc
     3181 atatcaacat tgcctggcag gacgtagata ccgttctgct ggatatggac ggcacgttgc
     3241 tcgacctcgc cttcgataac tatttctggc aaaagctggt gcctgaaaca tggggcgcga
     3301 aaaacggagt tacgccacag gaagcgatgg aatatatgcg ccagcaatat cacgacgtac
     3361 agcatacgct aaactggtac tgtcttgatt actggagtga gcaactgggt ctggatatct
     3421 gtgcgatgac caccgagatg ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc
     3481 ttgaggcact gaaagccagc ggtaaacagc gaattttgct caccaatgcg catccgcaca
     3541 acctggcggt aaaacttgag cataccggtc tggacgcaca ccttgattta ttactttcca
     3601 cccacacatt tggttatccg aaagaggatc agcggttatg gcatgcggtg gccgaagcta
     3661 cgggtcttaa agctgaaaga acgctgttta ttgatgacag cgaagcgatt ctcgatgctg
     3721 ccgcgcaatt tggtattcgt tactgcctcg gtgtgactaa tcctgactcc gggattgccg
     3781 agaaacagta tcaacgccat ccgtcactga atgactaccg ccgcctgatc ccctcgctaa
     3841 tgtgaaggag atgccatgaa agaaaaacct gctgttgagg ttcgactgga taaatggcta
     3901 tgggctgccc gtttttataa aacccgcgcg ctggcccgtg aaatgattga aggcggtaag
     3961 gtgcattaca acgggcagcg cagcaagcca agcaaaatcg tcgagctgaa tgccacgctc
     4021 actctgcgcc agggaaatga cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt
     4081 cgccccgcca gcgaggcagc cttgctgtat gaagagactg cggaaagtgt agagaaacgc
     4141 gaaaaaatgg cgctggcacg taaacttaat gccttaacca tgccgcatcc ggaccgacgc
     4201 ccggacaaaa aagagcgccg cgacctgtta cgatttaaac acggcgacag tgaataactg
     4261 tcacctgcaa gagagatgat tatgccgcaa catgaccaat tacatcgcta tctgtttgaa
     4321 aactttgccg ttcgcggcga actggtaacc gtttcggaaa ccctgcaaca gatccttgag
     4381 aatcacgatt atccgcagcc cgttaagaac gtgctggcag aactgctggt tgcgaccagc
     4441 ctgttaaccg ctacgttgaa gtttgatggt gatatcaccg tacagctgca aggcgacggt
     4501 ccgatgaatc tggcggttat taacggtaac aacaaccagc agatgcgcgg tgtggcgcgc
     4561 gtgcagggcg aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg
     4621 gtgatcacca tcaccccgag cgaaggcgaa cgctatcagg gcgtggttgg tctggaaggt
     4681 gataccctgg cggcctgcct ggaagattac tttatgcgtt ctgaacagct gccgacgcgc
     4741 ctgtttattc gcaccggcga cgtagacggc aaaccggctg caggcggaat gttgttgcag
     4801 gtaatgcctg cgcaaaatgc ccagcaggac gactttgacc acctggcgac gctaaccgaa
     4861 accatcaaaa ccgaagaact gctgacttta ccggcaaacg aagtgttgtg gcgtttgtat
     4921 cacgaagaag aggtgacggt ttacgatccg caggatgtgg agttcaaatg cacctgctcg
     4981 cgtgaacgtt gcgccgatgc gctgaaaacg ctgcctgatg aagaagtcga tagcatcctg
     5041 gcggaagatg gcgaaattga catgcattgt gattactgcg gtaaccacta tctgttcaat
     5101 gcgatggata ttgccgagat ccgcaacaac gcgtctccgg cagatccgca agttcattaa
     5161 tgagtatgtc cggcagagag gactctgccg gattttacga ctaatacacc tgatgcgctt
     5221 tgtcactcat tcagattcac atccagcctt tcaccatcca ctacaagatc gcgccacgct
     5281 ttaggcctcg tccccagctt tccatccttc gcagctgtga gtaatttttc tttcgttatt
     5341 ttgcgcggaa gtaaactcat atcaagcgcg tcccattctt ttctggttga gtcaaagcca
     5401 tagacaatga ctctgtcatc attaaaagca aacaggatcc gctccgcctg accatcacta
     5461 ttcagatctt gctcgaccag tacgcaggcg tcttttccaa tacaggtcat cacgttatag
     5521 cgatcctgga ttaaggccga ccagaatgtc gcatccggtt taccagaacc tggggcaatt
     5581 aacacattat cggctaatac tttttccgat acctgttcct gaagatgttg ctctccatct
     5641 aacgccatca gcagatcccg cgcgcgtttc gggtctttca tatactcggc atcgcttttc
     5701 agcgactcaa gcgccgcacg tccatagcga ccgctctgtt cgagcatgta gatagttacc
     5761 tggtctggcg tgtttttgcc gctctgataa cgcgccatat gactgttcac gctaatgcgc
     5821 atactgtcca gcaccggcga attaagaagc accagtatta ccaacaccaa taacgaaacc
     5881 acaaggttca ctttgccctg aagatcaagg ggattttgcc ctttacgcca gacgatactg
     5941 acaaaatacc ccagcgacca taccagtaac accagcactg ccagcgcgcc ctgcaagcgg
     6001 tcggcagtcc agccgtactg agcgacccgc agccataacg cccaggcggc gacgaacaca
     6061 tacagcggag caaccagcaa agcggtttta atcaggcaac gcaatggccc cgtccaggga
     6121 agtgacgctt tttgcggatc gcggacaata gccattaaga tcaattgcaa aaaggccagc
     6181 gttaacagca acccagcggc ggaaatgtgg cgagaaatcg cgctcaggcc cgtaaacggc
     6241 agggtgataa taaacatcag ggttagcaat gatactaacg gcagcaaccc cgtggcgatt
     6301 aacgtgaaca acttttgaat agagtcgatt aaacgtgact gtgtacgcgc gaggatcacc
     6361 gccagtgcgg taaccagacc taacgtgaga taaataaacc cgtcggttgc aaaaaagagc
     6421 gttttgaaaa acgtgatgcc aacaagttta aacaactctc cccacagtaa aagtaccagc
     6481 cacgtcaggc cattggcgag aaaaatcacc agtaatataa gtaagttatg ccatactgac
     6541 tggtaaaaat agcgatagcg gctactgtca ttgcggatgc gaaggctttg ttgtatccac
     6601 ggtagcaaca acattgccat cagcagcaga tagcagccaa aatcccaaag cgccttttct
     6661 gctctccagt ggttcatgcc atcaatttgc cactttagcc aaccgctcat tcccagcgtg
     6721 gcaataaaca ccagcgccag ccatccccaa aggcgcttct gtttaaagga gatcacggag
     6781 aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat ccaactgtga
     6841 ttttttccgg caagccaggc gatcagcagg tagcaaacca gcccctgaag taatccggta
     6901 gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt tatccatgtt agttatccag
     6961 aatcaaaagg tgggttaatt atcgcatccg ggcagtagta ttttgctttt ttcagaaaat
     7021 aatcaaaaaa agttagcgtg gtgaatcgat actttaccga ttgaatttgc atcaatttca
     7081 ttcatgaatg cgattccact cacaatattc ccgccatata aaccaagttt taaccttttg
     7141 agaacatttt ccacacctaa aatgctattt ctgcgataat agcaaccgtt tcgtgacagg
     7201 aatcacggag ttttttgtca aatatgaatt tctccagata cgtaaatcta tgagccttgt
     7261 cgcggttaac acccccaaaa agactttact attcaggcaa tacatattgg ctaaggagca
     7321 gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact cgaggcttat ggtatcagtg
     7381 acgtacatga tatcgtttac aacccaagct acgacctgct gtatcaggaa gagctcgatc
     7441 cgagcctgac aggttatgag cgcggggtgt taactaatct gggtgccgtt gccgtcgata
     7501 ccgggatctt caccggtcgt tcaccaaaag ataagtatat cgtccgtgac gataccactc
     7561 gcgatacttt ctggtgggca gacaaaggca aaggtaagaa cgataacaaa cctctctctc
     7621 cggaaacctg gcagcatctg aaaggcctgg tgaccaaaca gctttccggc aaacgtctgt
     7681 tcgttgtcga cgccttctgt ggtgcgaacc cggatactcg tctttccgtc cgtttcatca
     7741 ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt tattcgcccg agcgatgaag
     7801 agctggcagg tttcaaacca gactttatcg ttatgaacgg cgcgaagtgc actaacccgc
     7861 agtggaaaga acagggtctg aactccgaaa acttcgtggc gtttaacctg accgagcgca
     7921 tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa gaaagggatg ttctcaatga
     7981 tgaactacct gctgccgctg aaaggtatcg cttctatgca ctgctccgcc aacgttggcg
     8041 agaaaggcga tgttgcggtg ttcttcggcc tttccggcac cggtaaaacc accctttcca
     8101 ccgacccgaa acgtcgcctg attggcgatg acgagcacgg ctgggacgat gacggcgtgt
     8161 ttaactttga aggcggctgc tacgcaaaaa ctatcaagct gtcgaaagaa gcggaacctg
     8221 aaatctacaa cgctatccgt cgtgatgcgc tgctggaaaa cgtcaccgtg cgtgaagatg
     8281 gcactatcga ctttgatgat ggttcaaaaa ccgagaacac ccgcgtttct tatccgatct
     8341 atcacatcga aaacatcgtt aagccggtgt ccaaagcggg ccacgcaact aaagttatct
     8401 tcctgaccgc agacgccttt ggtgtgctgc cgccggtttc tcgtctgact gccgatcaaa
     8461 cccagtacca cttcctctct ggcttcaccg ccaaactggc cgggactgag cgtggcatca
     8521 ccgaaccgac gccaaccttc tccgcctgct tcggcgcggc gttcctgtcg ctgcacccaa
     8581 ctcagtacgc agaagtgctg gtaaaacgta tgcaggcggc gggtgcgcag gcgtatctgg
     8641 ttaacaccgg ctggaacggc actggcaaac gtatctccat taaagatacc cgcgctatta
     8701 tcgacgccat cctcaacggt tcgctggata acgcagaaac cttcactctg ccgatgttta
     8761 acctggcgat cccaaccgaa ctgccgggcg tagacacgaa gatcctcgat ccgcgtaaca
     8821 cctacgcttc tccggaacag tggcaggaaa aagccgaaac gctggcgaaa ctgtttatcg
     8881 acaacttcga taaatacacc gacacccctg cgggtgccgc gctggtagcg gctggtccga
     8941 agctgtaatg atttgaagct ggagaatatc tatccagtat cttatagaaa gcaaaacggg
     9001 aggcaccttc gcctcccgtt ttatttaccc ttcttttgtc atgccctgcg cccgcgttac
     9061 cggcactggc agccaggcgc gaatggaaag cccgccccgt tcgctggtac caagctccag
     9121 catgccgtta tggttatcca cgatacgctg cacaattgcc agccctaatc ccgtgccgct
     9181 aatggtgcgc gcactgtcgc cgcgaacaaa cggctggaac aggtgcttac gttgttccgg
     9241 cgcaattccc ggaccatcat cttccacctg aaaccaggcg cgattcggct ccgttccgct
     9301 gctgactttg atccagccat tgccatagcg ggcagcgttc accaccatat tcgccaccgc
     9361 acgtttgatc gacagcgggt gcattttcac ctcaatgctg ccggggtaaa gcgcggtttc
     9421 aatttcccgc tcatagccac tttcggcagc aatcacctca ccgagcactg cgttgagatc
     9481 cgccatttcc atcggcatct cctgcccggt gcgcaggtag tcgataaact gctcaatgat
     9541 ggcgttgcac tcttcgatat ctttattgat cgattcggcc aagtagccat cctgctcgct
     9601 catcatctcg gtcgccaggc gaatgcgcgt cagcggtgta cgcagatcat gacttacccc
     9661 cgccatcagc agcgtgcgat catccgccag ttgcttaaca ccagccgcca tatggttaaa
     9721 ggcgcgggta acggaacgca cctccgaagc gccatattca cgcagcggcg gcggaataat
     9781 ccctttaccg acctgcagag cagcatgttc gagatcgacc aacggtcggt tctggatacg
     9841 gataaacagc cacgccccgc ctatcgccaa tagcataatc gccagcgtat agcggaacag
     9901 cggagaaaaa tcaccctgat gaatttcggt cagcggcacg cgcacccaga tattgggcga
     9961 cagccaggtt ttcagccaga caacaggcga acttttgttg acctcaacgc gcacttccgt
    10021 cgggccgccc agttgctgcg ccatctgatg gcttaagaat tcatagtgtt gtgcccaacg
    10081 cagacctgcc tcttcggcag cttcgttgga atagagagag atccccagct cacggtagat
    10141 ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc
    10201 ggtcatcaac atacgcactt cgtacgcgag gactttatta aactgctgga ggctcggcaa
    10261 aatcgcgaag ttcagcacca ccagataagt cgtcaccagg ctgacgaaca gcaaggtgac
    10321 gatgagcaat aacgtacggg caaatgaact tcgtggcgag aagcgcaatc gcctcatgct
    10381 ttagaaccgt ccggtacaaa gacgtagccc agaccccaga cggtctgaat gtaacgcgga
    10441 tgcgccggat cttcttccac catgcggcgt agacgcgaaa tctgcacgtc gatggagcgt
    10501 tccattgcgg agtattcacg gccacgggca aggttcatca gcttatcgcg ggagagcggc
    10561 tcacgcggat ggctgaccag tgccttcagt accgcaaact caccgctggt gagcggcatc
    10621 ggctcatctt cgcggaacat ttcacgcgta ccgaggttaa gtttgaactt accgaaagca
    10681 attaccgcct cttcctgtga cggcgcgccc ggcagttcgt tggcctgacg acgcagcacc
    10741 gcacgaatgc gggccagcaa ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg
    10801 ccaatctcca ggccaacgat acggtccact tcttcccctt tcgccgtcac cataatgatc
    10861 ggcatcgggt tgctctggct acgaagacgt cggcaaatcg acaagccatc ttcaccaggt
    10921 aacatcaaat ccagtaccat aagatggaaa gattcacgag tcagcaggcg atccatctgt
    10981 tctgcattag cgacgcttcg aacctggaag ccttgttcgg tgagataacg ttccagcagc
    11041 gcgcgcaggc gcatatcgtc atcgaccacc agaatcttgt agttctcttg cattgtttgt
    11101 actcccaaag gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc acgttcgtca
    11161 ccagctaaat ctggtatgaa tttcagccta aattgttaca aagcatatta aacagcagct
    11221 taagtataca atttattcgg cgaaacatta ttgattctgt tgatatgatc acgttatacc
    11281 caatgtgcgc attatcaaac agacaaaggg aatcaacgag atgaaaacgc ccctggttac
    11341 ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg aagaacgccc
    11401 ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg aaaatgctga
    11461 ctatcagtat aataaaaagc gcctgcgtga aatcgaccgt cgcgtgcgct atctcactaa
    11521 atgcatggaa aatctcaaaa tcgtcgatta ctccccgcag caggaaggta aagtcttttt
    11581 tggcgcgtgg gtggagattg aaaacgacga tggcgtgact caccgtttcc gtattgtcgg
    11641 ctacgatgaa atttttggcc gtaaagatta catctctatc gattccccga tggcccgcgc
    11701 attgctgaaa aaagaagtcg gcgatctggc ggtggtgaat acccctgccg gggaagcgag
    11761 ctggtatgtt aatgctatcg agtacgtgaa accgtaagga agagtcttaa cctcctgccg
    11821 atggctggca tttttgccag ccagtccgta taactatccc ctgatttttg atccgaaaag
    11881 atgaactcaa accatgatga atgattcgtt ctgccgcatt attgcgggtg aaattcaggc
    11941 gcgcccggaa caggttgacg ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt
    12001 tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat gacacgcagc tgcgtaatct
    12061 ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc
    12121 catttccgag caaggcaaac tcactgatga tctggcgaat gccatcaacg ccaccttaag
    12181 caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct aaacgccgca cccgcgggca
    12241 aatcgccatt gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca
    12301 cacgccagaa gtcgcggctg cacaatatgt tgatgccgat aaaggcgtgg cagataccaa
    12361 agccgcgctg gacggcgcgc gctatatcct gatggaacgg tttgccgaag atgccgcgct
    12421 gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat ttggtttcga cggtggtgag
    12481 cggtaaagaa gaggaagggg cgaaattccg cgactatttc gatcatcacg aaccgctgtc
    12541 cacggtgcct tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag gcgtacttca
    12601 gctttcgctg aatgccgatc cgcagttcga tgagccgcca aaagagagct attgcgagca
    12661 aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa
    12721 aggtgtggtg agctggacgt ggcgcatcaa ggtgttgatg catctggaaa ctgaactgat
    12781 gggcaccgtg cgcgaacgcg cggaagatga agcaatcaac gtctttgccc gtaacctgca
    12841 cgatctgctg atggcggccc ctgccggact acgtgcaacg atgggcctcg atccgggtct
    12901 gcgtaccggg gtaaaagtag cggtagtcga tgcgacaggt aaactggtgg cgaccgatac
    12961 catttatccg cataccgggc aagccgcaaa agcagcgatg accgttgctg cgctgtgtga
    13021 aaagcataac gttgaactgg tggcgatcgg caacggtaca gcttctcgcg aaaccgaacg
    13081 tttctatctc gacgtgcaga agcagttccc gaaagtgacc gcgcagaaag tgatcgtcag
    13141 cgaagctggc gcgtcggttt actcggcttc cgagctggca gcacaggaat tcccggatct
    13201 cgacgtttcg ctgcgtggcg cggtgtctat cgcccgtcgt ttgcaggatc cgctggcgga
    13261 gctggtgaaa atcgatccga aatctatcgg cgtaggtcag tatcagcatg acgtcagcca
    13321 gacgcagctg gcacgcaaac tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt
    13381 cgatctcaac accgcttctg ttcctctatt aacccgcgtg gcgggcctga cgcgcatgat
    13441 ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag ttccagaacc gtcagcaact
    13501 gttaaaagtg agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct tcttgcgcat
    13561 taaccacggt gataacccgc tggacgcttc taccgttcac ccggaagcct atccagtggt
    13621 ggaacgcatt ctggcagcaa cacagcaggc actgaaagat ctgatgggta acagcagcga
    13681 actgcgtaac ctgaaagcat ctgactttac tgatgagaaa ttcggtgtgc cgacggtaac
    13741 cgacatcatc aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc
    13801 tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg
    13861 tgcggtgacc aacgtcacca actttggcgc gtttgtcgat attggtgtgc atcaggacgg
    13921 cctggttcac atctcttcat tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa
    13981 agcgggcgac attgtgaagg tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc
    14041 cctgaccatg cgtctggacg agcagcctgg cgaaaccaac gcccgtcgcg gtggcggtaa
    14101 cgatcgcccg caaaacaacc gcccggcagc caaaccgcgt gggcgtgaag cgcagcctgc
    14161 cggtaatagc gcgatgatgg atgcgctggc ggcggcaatg ggcaaaaaac gttaaacgcc
    14221 cggactggcc cctaaacctg cgcgattagg cgttcacgcc gcatctggca atgaacgcct
    14281 tgcctgacat aaaagtgccg gagaatatct ccggtctttt tattccacaa gcaaactcat
    14341 aatagattcc gttaatattt atcacttcat taacaactga aaccttaatt aaacattaac
    14401 cagccctaat aattcgtcat gcgaattata ttttcgctgc gatataacct tgagccatat
    14461 caacattgag tcagattatt attcaaacca acattcacac acattttaag tattgctgat
    14521 agaaaccatt ctcattatca ttgtactgtt gattatttaa tctctccttc gttggcaaat
    14581 catctggtct catgtcgctg tcaaacgccc catgaggtag ttatccagtt aatgagaaac
    14641 aagtaggcac ctatgcaata cactccagat actgcgtgga aaattactgg cttttcccgt
    14701 gaaatcagcc cggcatatcg ccaaaaactg ctttctcttg gcatgttacc tggctcctct
    14761 tttaatgtgg tgcgcgtcgc tccactcggc gaccccattc atatcgaaac ccgtcgtgtg
    14821 agcctggtat tacgcaaaaa agatctggcc ttattagaag tggaagcggt ttcctgttaa
    14881 tacggtgata acaacaatga aaaaattaac cattggctta attggtaatc caaattctgg
    14941 caagacaacg ttatttaacc agctcactgg ctcacgtcag cgtgtaggta actgggctgg
    15001 cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc gatcatcagg tcacgctggt
    15061 ggacctgccc ggcacctatt ctctgaccac catctcatcg cagacctcgc tcgatgagca
    15121 aatcgcctgt cactacattt tgagtggcga cgcagacctg ctgattaacg tggtggatgc
    15181 gtctaacctt gagcgtaacc tgtacctgac gctgcaactg ttggaactcg gcattccctg
    15241 catcgtggcg ctgaacatgc tcgacattgc cgagaagcaa aatattcgca ttgaaattga
    15301 tgctctgtcg gcgcgtcttg gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg
    15361 tattgaagcg ctcaagctgg cgattgatcg ctataaagct aacgaaaatg tggaactggt
    15421 gcattacgct cagccgctgc tcaacgaagc agattcactg gcaaaagtga tgccttccga
    15481 catcccgctg aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg atatctacag
    15541 ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc gccctcgccc gtctgcgtaa
    15601 tgagatggac gatccggcgc tgcacattgc cgatgcgcgt taccagtgca ttgctgccat
    15661 ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc cgtttcacca ctgcggtaga
    15721 taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc ctctttgtga tgtacctgat
    15781 gttcttgctg gctatcaaca tcggcggggc gttacagccg ctgtttgacg tcggctccgt
    15841 agcactcttt gtgcatggta ttcagtggat tggctacacg ctccacttcc cggactggct
    15901 gactatcttc ctcgcccaag gcctgggcgg tggtattaac accgtgctgc cactggtgcc
    15961 gcagatcggc atgatgtacc tgttcctctc cttccttgag gactccggct atatggcgcg
    16021 tgcggcgttt gtgatggacc gtctgatgca ggcgctgggc ttgccgggga aatcctttgt
    16081 accgctgatc gttggtttcg gttgtaacgt accgtcggta atgggcgcac gtacgcttga
    16141 tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg
    16201 tctggctatc ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg cgctggcggt
    16261 cttctcgctg tatatgctgg gtatcgtaat ggcggtgctg acgggcctga tgctcaagta
    16321 caccatcatg cgcggtgaag cgacgccgtt tgtcatggag ctgccggtct accatgtacc
    16381 acacgttaaa agcctgatta tccagacctg gcagcgtctg aaaggcttcg ttctgcgtgc
    16441 tggtaaagtg attatcatcg tcagcatttt cctgagcgcc ttcaacagct tctcgctgag
    16501 cgggaaaatc gtcgataaca tcaacgactc ggcgctggct tccgtcagcc gggtgatcac
    16561 cccggtcttc aagccgattg gcgtgcatga agataactgg caggcaacgg ttggcctgtt
    16621 tacaggtgcc atggcgaaag aagtggtagt gggtacgctc aacaccctct acaccgcaga
    16681 aaatattcag gacgaagagt tcaatccggc ggaatttaac ctcggtgaag agctgttcag
    16741 tgcggtagat gaaacctggc agagcctgaa agacaccttc agccttagcg tactgatgaa
    16801 ccccattgaa gccagcaaag gcgacggcga aatgggtacc ggggcgatgg gcgtgatgga
    16861 tcagaaattc ggtagcgcag ctgccgcata cagctacctg attttcgtcc tgctgtacgt
    16921 accgtgcatc tcggtgatgg gggctatcgc gcgtgaatca agccgtggct ggatgggctt
    16981 ctccatcctg tgggggctga atattgctta ctcactggca acattgttct atcaggtcgc
    17041 cagctacagc cagcatccaa cttacagcct ggtgtgcatt ctggcagtta tcctgtttaa
    17101 catcgtggtt atcggtctgc tgcgccgcgc gcgtagccgg gtagatgtcg aactgctggc
    17161 aacgcgcaaa tcggtaagca gttgctgtgc ggccagcacc accggtgatt gccattaatg
    17221 gcttcactta ttcaggtgcg tgatttgctg gcgttacggg gccgtatgga agcggcccag
    17281 attagccaga cactgaacac tccgcagcca atgattaacg ccatgctgaa acagctagaa
    17341 agtatgggaa aagccgtgcg gattcaggaa gaacctgacg gctgtctttc cggcggttgt
    17401 aaaagctgcc cggaaggaaa agcctgtctg cgcgaatggt gggcgttgcg ttaaccttgt
    17461 ttcactgcct gttttgaaag cccggtatgc gtttgcatcc gggctttttt gcgtgcggct
    17521 ttccataaaa atgcaaccct tgcagcgcga cgtaagttcc tctgaaagcg tctcgcaggg
    17581 atgaaaactc gctaatgcac aggtgtggag tggcgcgtag agtcgcagca ttcaaataac
    17641 aggtgaagga acgccatgag caaaaagcag agttccaccc cacacgatgc gttacgccat
    17701 tgccgcaatg caaaatcacc ttgatgccgg atacaaaaca ttgccgatgg tggtgccatt
    17761 gttgttttat cacggtattg aaagccccta tccctattcg ctgtgttggc tggattgttt
    17821 cgccgatccc aacctggcaa ggcagcttta tgcctctgca tttccgctga ttgatgtcac
    17881 cctcatgcct gatgatgaaa tcatgctgca ccgacgtatg gcgctactgg agttaattca
    17941 aaaacatatt cgtcaacgcg acctgatggg gctggtagag caaatggcct gcttattaag
    18001 tagtggatac gctaatggca gacaaatcaa agggctgttt aattacatac tgcaaaccgg
    18061 cgacgcggta cgttttaacg attttatcga tggtgtggcc gaacgttcac caaaacacaa
    18121 ggagagttta atgactattg cggaaagatt gcggcaggaa ggggaacaat ccaaagcctt
    18181 gcatatagca aaaataatgc ttgaatccgg agtccctctt gcagacatca tgcgctttac
    18241 cggggtgtca gaagaagagt tggctgcggc gagccagtaa agttctgtcc cgccatttcg
    18301 aaagccacct acaccctctg cttcaacgcc accagcagat gacaaaactc gtccggatgt
    18361 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
    18421 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
    18481 aaaaacggca tgggcacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
    18541 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
    18601 gcccgcgcat cctggcgcgc cgtttcagta cccatggttt gtaacgccag gaaccgctcc
    18661 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
    18721 atccccggcc actcgtcgcg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
    18781 tgaacccgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
    18841 cctaaccaaa tggctttatc tggtgcctgt cgcagcacga cttcggccat atcagcaagt
    18901 gatagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
    18961 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
    19021 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
    19081 cgctattgtt ctctttttac ttacaaggat gaacatatgc taacagtacc gggattatgc
    19141 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgctctcgc
    19201 gccgcccgta cagataaaac gttatgccca caatgtggat tacccgccac acactctcat
    19261 cttccctgcg gtcgctgcct gcaaaaaccg ctgccctggc aaagactggt cacggtcgcc
    19321 gactatgcgc cgccattaag tccgctcatt caccagctta agttttcccg gcgcagtgaa
    19381 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
    19441 ggcttgcaac tgcctgatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
    19501 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggct acactgccaa
    19561 tgggatagcg aagccgtcac acgaacacgg gccaccgcga cccagcattt tcttaacgcc
    19621 cggctacgca aacacaacct gaaaaatgcc tttcgccttg aattgcctgt gcaaggtcgc
    19681 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
    19741 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
    19801 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
    19861 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
    19921 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
    19981 ttcttattgc ccgccggacg ctgtggaagc caccgacacc gccctgaaat ttgacctgct
    20041 gaccgcgtat gttgatgagt taagcgctcc ttacctggaa gatgcagaga tcgattttgt
    20101 taccgaccag ttgggttcgc agctgacgct gaaagcgcct aacgccaaaa tgcgtaaagt
    20161 ggctgacgat gcgccgctga tggagcgcgt ggagtatatg ctgcaatcac agatcaaccc
    20221 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
    20281 cattttgcaa tttggcggtg gctgtaacgg ttgttccatg gtcgacgtca ccctgaaaga
    20341 agggatcgaa aagcagttgc tgaacgaatt cccggagctg aaaggtgtac gcgatctcac
    20401 cgaacaccaa cgcggcgagc actcttacta ctaagttctc tccccttttg tcgattgccc
    20461 gatgtgacgc taaagcgtct tatcgggcct gtacgttgca cgagagtctg acgttagcgc
    20521 agaattacct cagcaaatcc aatctcttcg tttccccacg ataatatgac caacctctca
    20581 taatttaaat ttaccccgct ctggtgattc tcaagcacgt aatgttaccc gtatcattct
    20641 catgggcacc aaacacactc ataacatctg actacactaa ctcgttttag gggtcatcag
    20701 ccgtggtgcc gtaacatctc tgttcccagt tgggacaacc aggattttcc gtcagaaacc
    20761 tgacgttacc cataacaaat taaaggccag gtaaatcatg ccattagtca ttgttgctat
    20821 cggtgtaatc ttgttattac tcctgatgat ccgcttcaaa atgaacggct tcatcgctct
    20881 cgtcctcgtg gcgcttgctg ttggattaat gcaaggaatg ccgctggata aagttattgg
    20941 ctccatcaaa gctggtgtcg gcggaacgct cggtagcctt gccctgatca tgggttttgg
    21001 tgcgatgctg ggcaaaatgc tggcagactg cggtggtgca caacgtatcg ctaccacact
    21061 gattgccaaa tttggtaaaa aacacatcca gtgggcggtg gtactgaccg gttttaccgt
    21121 tggttttgcc ctgttctatg aagtgggttt cgtgctgatg ctgccgctgg tgtttaccat
    21181 cgcggcttcc gcgaatattc cactgctgta tgttggtgta ccaatggcgg ctgcactgtc
    21241 tgtgacccac ggcttcctgc caccgcatcc gggtccgact gcaattgcca ccattttcaa
    21301 tgccgatatg ggtaaaactc tgctgtacgg tactattctg gcaatcccga ccgtgattct
    21361 cgccggtccg gtttacgctc gcgtgctgaa aggtatcgat aagccgatcc cggaaggtct
    21421 gtacagcgcg aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac
    21481 ctctctggtg ccggtggttc tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa
    21541 aggtcacgct ttcctgccgg tggcggagtt cctcggtgac ccggtaatgg ccacgctgat
    21601 tgccgtgctg attgcgatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat
    21661 taacgacacg ctggtttctt ccatcaaaat catagcgatg atgctgttga ttatcggtgg
    21721 tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat
    21781 gatgcacgaa accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg
    21841 tatcgcgctg ggttccgcaa ccgtcgcggc aatcactgcg ggtggtatcg cggcaccgct
    21901 gattgcaacg acgggtgtta gcccggagct gatggttatt gcggttggtt ccggtagtgt
    21961 gattttctct cacgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac
    22021 tatcggcgag accatcaaat cctggtcgat gctggaaacc attatctcgg tgtgcggtct
    22081 ggtaggctgt ctgctgctga atatggtgat ttgataatac tgatggccgg atgcggcgtg
    22141 aacgccttat ccggcctacg attcggcatt aattagtagg ccggataaga cgcgtcaagc
    22201 gtcgcatccg gcatcaaccg cactctactt cttcttcgct gccgctctgc gccgtctgtc
    22261 caaatccttc agcaacttgt tcacaccatc atcggcaaac atcgactcaa gcgttgcgga
    22321 aagtttacgt cgccagtttt tatactggta actggtgccg ggaatattta ccggatctgc
    22381 catatccagc cagtcttccg gctgtagccc taacagggcg ctgttgctat cggcgatgta
    22441 gcgctgtaag ccacgattca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc
    22501 acgttttggc agacaaccat atttatgcag agcatccagt agtccttgct tcgccagttc
    22561 gcgatcctga tacagaccgc gcagtaccac ttcatccgga tacaacccca gcgttttgcc
    22621 cagcgttaga tccccgctct cccagtagcc gcgtagcgta ggcaggtcgt gtgtcgccgc
    22681 aaccgccatc gactgctctg ggtacgcttt cggcgcgcgg aatgtcttct cgtggtcgtt
    22741 ttcgaaatag agcactttgt aggagtacac accgctgctg cgcagcttac cgacaatctc
    22801 taccggtacg gtaccgagat cttcaccaat caccatacag cgatgacgct tactttcgag
    22861 tgccagaatc gagagcagat catccaccgg atagtgaaca tatgccccct gatctgccgt
    22921 ctcgccatac ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc
    22981 gccgcagttt tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt
    23041 gataatatgc gggtccattg gcggcaatcc ccagttctgc cccaacgggc cgaggatatc
    23101 cggcggtgct ccaaccgacg ctttcaggca atacaactcc cggtcgcacc aggtttctgc
    23161 tccaccctct gctacaccaa cggccaggtc acgatacaag ccaatcggca tttcatagcc
    23221 ctggcttatc tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata
    23281 aaaatcaacg tcatcgcgat gttcttcgca gaactggcgc acttctggtg aatccacgtt
    23341 ctgatacatc tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg
    23401 ggcatgtagt gcgtcaaacg ccgcctgcca gaacaggctg tcgccttgct ctgcaacaaa
    23461 ctggcgaaac gcggccatct gttcatcatc acgttgcgcg aaacctttcc acgccattcg
    23521 taatgctgtc atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcccgcgc
    23581 ctgttgcagc gtctgttgcg tggtcggcaa cttccaccag gcctgagcct cttcgctaag
    23641 atggaaatct tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga
    23701 cgggctatac gggctggaac tttccggatt tgccggatag agcgcatgaa tcgggttcag
    23761 accgatgaac gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa
    23821 atccccaata ccccagtttt tttccgatcg cagtgtataa agctgaacgc aggcacccca
    23881 cagcttttgt ttgttcagca acgcctgcgg ttcgtaacag cgtttcgggg cgacaatcac
    23941 ccggcaatgc gcgcgctggt cgtcctgggt aagcgtcagc gtgtgataac cttccggcag
    24001 cttcgtcggt agattgaacg ctttgccccc catcacatgg cctttgtact gctttccttc
    24061 ttcggtggtc agcagccagc tatattcgcc gctgccctcc accaccatcg gcattttttt
    24121 gccgctggta taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg
    24181 ttgatgcatc gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc
    24241 gtgggcattg atgtaattgg ggctgatccc cgccgccagc gcggcattat ccagacgttt
    24301 gctttccatc gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat
    24361 agagcgatcc gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca
    24421 ggcctcctgg tcgcggtaca gcacatccac ctgcttttgt gcctctacat aagcggcgaa
    24481 atctgccatc accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc
    24541 gaaggcatgc ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc
    24601 cagcacctta tctttcttcc gccatttcac cgggtcgtag cctttggcca gaattgcctt
    24661 cacttgttcc acggtatgac caaaaataaa gatattttct tcaccgactt tctcagcgat
    24721 ttcaacgttc gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat
    24781 attgccggta ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc
    24841 cgggatcagt ttttccgccg ccgaaacaca ataatccggc aagaaaacca ccttcaactt
    24901 gtcgccaacc agcggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat
    24961 atttttcgcc aggtagtagc ccggtgctgc tttcgcgccg aagaggaaga cgcgcggtac
    25021 gcggtcagac tgcgggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt
    25081 cagatgctgg cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg
    25141 attgatctca ataccggtac gaactttcac aaactctgcc agacggactt tattcgcctg
    25201 cttgatctcg cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat
    25261 cagttgatcg agatcgttag cccactcttt tttcagtgat ttatccaaca gagccgccag
    25321 tgccgggttg cactgtttga tccagcgacg tggggtaata ccgttggtga cattatggaa
    25381 tttgttcggc catagctggt gatattccgg gaacagatct ttcaccacca gatctgagtg
    25441 cagcgccgca acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac
    25501 ttgtttgtcg tgtaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt
    25561 ctccaccagc gttttaaagc gagtattaat ttcgttaata atctgcatgt ggcgcggcag
    25621 taagcctttc accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt
    25681 ggtgtaagcg aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc
    25741 atcgatcagc acgcgcagca gttctggaat cgcgatagtt gggtgggtat cgttcagctg
    25801 aataacttcg taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa
    25861 aatatccgct accgagcagg cacactggaa gtattgctgc atcaggcgca gctttttacc
    25921 ggcagtatgg ttgtcgtttg gatagagaac tttggtcagt ttttccgcgt tgatgccctg
    25981 ctgttcggca cgcaggaaat caccgtcgtt aaatttagtc agatcaaacg gatgcgcatg
    26041 cgtcgcctgc cacagacgca gtggctgcgc cacgccatta cgatagccga caacggggag
    26101 atcccacgct tgaccggtaa tggtaaactc cggctcccag cgtccatctt tcgtcacttt
    26161 accgccaatc cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttacc
    26221 gcgatgccag tcatccggcg cttcaacctg tttgccatcg acaaatgact ggcggaacaa
    26281 gccatattga taattaaggc cgtagccagt agctgactgc ccgacagttg ccattgagtc
    26341 gaggaagcac gccgccagac gtcccagacc accgttcccc agcgccgggt cgatctcttc
    26401 ttccaacagg tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata
    26461 ccagccgaga ttcaacaggt tgttgcccgt caggcgacca atcaaaaact ccattgagat
    26521 gtagttaaca tgtcgctgat tcgccactgg cttggcgaat ggctgagcac gcagcatttc
    26581 ggccagtgct tcgctcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt
    26641 taagccataa cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat
    26701 aggttgtgac ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt
    26761 gccaggctcg atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg
    26821 gatgagcaag gaaatgtgat ctcgaccact taaagctagt gcaatccaca ggattagcag
    26881 caaatcaatg ccataccgcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt
    26941 attttcaaaa agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa
    27001 gtaaaatatc aagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag
    27061 atggcacttt catcagaaat gaagaagaaa cccttgctta aatgaaactg atgaacataa
    27121 ggaaaatacc gtacaaccca gtattcacgc tggatcagcg tcgttttagg tgagttgtta
    27181 ataaacattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac gtcatcgctt
    27241 gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca aaaaaaataa
    27301 tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa aattaagtcg
    27361 tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc
    27421 gaacaacttc cggctggcgc tgatcacaag tcctgcgggc tacggaaaga ccacgctcat
    27481 ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggatg aaggtgataa
    27541 ccagcaagag cgtttcgcca gctatctcat tgccgccgtg caacaggcaa ccaacggtca
    27601 ctgcgcgata tgtgacacga tggcgcaaaa acggcaatat gccagcctga cgtcactctt
    27661 cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg tcatcgatga
    27721 ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta ttcgccatca
    27781 accagaaaat ctcacccttg tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa
    27841 tctgcgtgtt cgtgaccagc tgctggaaat tggcagtcag caactggcat ttacccatca
    27901 ggaagcgaaa cagttttttg attgccgtct gtcatcgcca attgaagccg cagaaagcag
    27961 tcggatttgt gatgatgttt ccggttgggc gacggcactg cagctaatcg ccctctccgc
    28021 ccggcagaat actcactcag cccataagtc ggcacgccgc ctggcgggaa tcaatgccag
    28081 ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg caacgcgcca
    28141 ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gcactcatca cccgtgtgac
    28201 cggcgaagaa aacgggcaaa tgcgcctcga agagattgag cgtcaggggc tgtttttaca
    28261 gcggatggat gataccggcg agtggttttg ctatcaccca ctgtttggta acttcctgcg
    28321 ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg ccgccgcaga
    28381 aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg cggcaggcga
    28441 tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg agtttgttca accatagcga
    28501 actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc
    28561 gcagttggtg ttattgcagg cgtggctgat gcaaagccag catcgctacg gcgaagttaa
    28621 caccctactg gcccgcgctg aacatgaaat caaggacatc agagaaggca ccatgcacgc
    28681 agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg atgaagcgga
    28741 acggctggca aaactggcac tggaagagct gccgccgggc tggttctata gccgcattgt
    28801 ggcaacctcg gtgctgggtg aagtgttgca ctgcaaaggc gaattgaccc gctcactggc
    28861 gttaatgcag caaaccgaac agatggcacg ccagcacgat gtctggcact acgctttgtg
    28921 gagtttaatc cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga
    28981 aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat ctggaacagc tgccaatgca
    29041 tgagtttctg gtgcgcattc gtgcgcagct gttatgggcc tgggcacggc tggatgaagc
    29101 cgaagcgtcg gcgcgcagcg ggattgaagt cttgtcgtct tatcaaccac agcaacagct
    29161 tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag ataacgcccg
    29221 tagccagctg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca gcgactggat
    29281 ctctaacgcc aacaaagtcc gggtgatgta ctggcaaatg accggcgata aagccgccgc
    29341 tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact tcctgcaagg
    29401 tcaatggcgc aacattgccc gtgcacaaat cttgctgggc gagtttgaat ctgcggaaat
    29461 tgtcctcgaa gaactcaatg aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg
    29521 taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg acgcccagcg
    29581 tgtgttgctg gacgcattaa aactggcgaa tcgtaccgga tttatcagtc attttgtcat
    29641 cgaaggcgaa gcgatggcgc aacaactgcg ccagcttatt caactcaata cgctgccgga
    29701 gctggaacag catcgcgcac agcgtattct gcgagaaatc aatcaacatc atcggcataa
    29761 attcgcccat ttcgatgaga atttcgttga acgtctgcta aatcatccgg aagtgccaga
    29821 gctgatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc tgatttactc
    29881 cggttacagc aatgagcaaa ttgcaggcga gctggaagtg gcggcaacca ccatcaagac
    29941 gcatatccgc aatctgtacc aaaaactcgg tgtcgcccac cgtcaggccg cggtacaaca
    30001 cgcgcagaaa ctgctgaaga tgatgggata tggggtatga gtttagccgg ataatgcgcc
    30061 agatccggct tacatcttta catcattcaa tgctcacccg cgttacgcca tctgtttcta
    30121 tcagactaaa ccgcactggc aagaaacgct ccaccaccgc gatattggtc tgcagatggc
    30181 atgagggatg ggcgaccgta aattctcccg cccccgccag cgccatcggt agcaccagtt
    30241 ggtcggcgag atattccccc accgccgccg ggcttgccag gtagcgtttc acctctttca
    30301 ccaactgtgc cgcgaccacc tcggcactga cgcgcttttc accgacgaca aaaaagcgtt
    30361 cggtgatatt ttcactttcg acttcaagcg agacggtatt acccggcccc tggtcgcgcg
    30421 gcaggttatg aatattctgt tcatgcaagg aaaaactacc cgccagtgta gcgatttcac
    30481 gctcagcaac atggcgcggc acaccagcca gtagcacctc tccacgcatc tgcacaatgt
    30541 tcccgcgctc gccaagttgc aagctattaa acgatgccac cggtgagact tccgttgcta
    30601 ccacgccgcc tccggcagga taaaaaccgt gacgtaacag cgtggtttgc tgatgaactc
    30661 ctattttcgc cagcagcggc tccagcaccc gacggataaa atccgcaggc ggggccgacg
    30721 ggttatcggt gccgccgctc acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg
    30781 gcagcaccgt ttgcagcacc agcgtacaac ttccggcgct accgatagca aagcggtaat
    30841 cgccgccgcg cacggtgcca ggtcggaaga ccagacgctg cgaccccagc tcagcgcctt
    30901 ccaccgttgc cctacaaatt tccgcagccg cttttaccgc ggtcagatgt tggcgcaaca
    30961 gccccggttt cgcccgcccg gcacgaatgc cggtgatggt aaatggctgg ccggttatca
    31021 tcgacaggct cagcgccgag cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca
    31081 gcgcaatcat ccttttcatc atttatcctt ttacgcacac cacctgacgc agggtataga
    31141 taacttccac cagatcgctt tgtgccgcca tcaccgcatc aatatcttta tacgccatcg
    31201 ggatttcgtc gatcacctcg gcatctttac ggcactccac atgcgcggtg gcacggattt
    31261 gatcttccac actgaacagt tttttcgctt tagtacggct cattacccgc ccggcaccgt
    31321 ggctgcacga acagaacgac tcttcatttc ccagcccacg gacgataaag cttttcgccc
    31381 ccatcgaacc gggaataatt ccatattgac cagcacgcgc agacaccgcg cctttacgcg
    31441 tcacatagat ctcttcgcca aagtgcagtt ctttttgcac atagttgtgg tgacagttga
    31501 tctcttccat cgccagcgtt tgtggctgtt ttaccgtttt ctgcataacg ctctgcaacg
    31561 ccgttaccac gttttccatc atcgcatcgc ggttaaggct ggcaaaaagc tgcgcccagg
    31621 ccacggcttt caggtaatca tcaaagtatt ccgtaccttc cataaagtac gccaggtcac
    31681 gcgatggcaa cgtttcaagc gtttcctgca tctctttttg cgccagatcg ataaaataag
    31741 ttccgatggc gttaccaatt ccgcgtgaac cggagtgcag cataatccac acctgatccg
    31801 actcatcaag gcaaatttca ataaagtggt tacccgtacc cagcgttccc aggtgtttat
    31861 agttattagt gttaaggaaa cgcggatatt tttgcgttaa ccactgataa ccggcctcca
    31921 gctcagccca tttcgcatcg acgttaacag gcggattttc ccatgcgcct ttatcacgtt
    31981 tacaacgccc ggtagtacgt ccgtgcggta cggccgtttc aatcgcctga cgcagctctg
    32041 ccaggttttc aggcaggtcc gccgccgtta acgcggtacg cagtgcgttc attccacagc
    32101 caatatccac gcccaccgcc gccggaataa tcgccccttt ggtcgggatc acgctaccaa
    32161 tggtggaacc tttacccagg tgtacatcag gcattaccgc aatatgtttg aaaataaatg
    32221 gcatcttcgc ggtattaata agttgctgac gcgcatcggc ttctaccggc acgcctttgg
    32281 tccacatttt taccggagca ttttcagtgg tcagtaattc gtaattcatt ttgtttttct
    32341 cttttcgttg tttgctgtcc tgataattgc aaccgtcgtg ccagaaaatc aaataagcat
    32401 cttaattttt taattcattg tttttaaaaa tattatattt ttacgtccgt aaacggagat
    32461 ttcccgcaaa gccgatttac cgataatgaa atctcgtctt ttataaggat atctaagatg
    32521 cgtaaaacag tggcttttgg ctttgtcgga acggtgctgg attatgccgg gcgcggcagt
    32581 cagcgctggt caaaatggcg tccgacactc tgtctatgcc agcaagaatc gttagttatc
    32641 gatcggctgg aattattgca cgacgcccgc tcgcgctcgc tattcgaaac gcttaaacgc
    32701 gatatcgcca gcgtttcgcc agaaacagaa gtggtgggcg ttgagataga actgcataac
    32761 ccttaggatt tcgaagaggt ttacgcctgc ctgcatgatt tcgcccgtgg ttacgcgttc
    32821 cagccagaaa aagaagacta tttaattcac atcaccaccg gtacccacgt cgcgcagatt
    32881 tgctggtttc tgctggcaga agcgcgttac ctgcccgccc ggttgataca atcttcacca
    32941 ccgcgcaaaa aagagcggcc cgatagccct ggcgcggtga cgattatcga tctcgattta
    33001 agccgttata acgccatcgc cagccgcttt gccgaggaac gccagcaaac gcttgatttt
    33061 cttaagtccg gcattgccac gcgtaactcc cacttcaacc gcatgattga gcagatcgaa
    33121 aaagtggcga tcaaatcccg cgcaccgatc ctgcttaacg gcccgaccgg tgcgggtaag
    33181 tcatttctgg cgcgacgcat ctttgaatta aaacaggcac ggcatcagtt tagcggcgcg
    33241 tttgtggaag tgaactgcgc caccctgcgc ggcgataccg ccatgtcgac gctgtttggt
    33301 catgtgaaag gcgcgtttac cggggcgcgg gaatcgcgtg aaggattatt acgcagcgcc
    33361 aacggcggaa tgttgtttct tgatgagatt ggcgaactgg gcgcagacga acaggcgatg
    33421 ttgctaaaag ccattgaaga gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc
    33481 agtgattttc agcttatcgc cggaacggtg cgcgatttgc gccagttggt tgccgaaggc
    33541 aaatttcgtg aagacctgta cgcgcggatc aatctctgga ccttcacctt gccaggatta
    33601 tgccaacgcc aggaagatat tgaaccgaac ctggattatg aagtggggcg ccacgcctca
    33661 ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc gtgcctggct ggcctttgcg
    33721 acctctcccc aggcaacatg gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg
    33781 atggccacct ttgccactag cggacgcatc acactggata cggtggaaga tgagatcaac
    33841 cgtctgcgct ataactggca ggagagtcgc ccctccacgc ttacggcgtt gctgggcgca
    33901 gaggcagaaa acatcgatct cttcgaccgt atgcaactgg aacacgttat cgctatctgc
    33961 cgccaggcaa agtcgctttc tgctgccgga cgtgaactgt ttgacatttc gcgccagggt
    34021 aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt tggtctgaca
    34081 tgggaagcaa tgcaggatca gcacagctcc agttgaatat gatggtcctt cagcacctgc
    34141 atcacgctcg caggcggcat cgtgtcggtg tagacagcat cgaccatgct gatgctaccc
    34201 atattgacca ttgcgttacg gccaaatttt gagtggtcga caaccagcat aacgtggcgc
    34261 gagttctcaa tgatggcgcg tttggtgcga acttcgtggt aatcgaactc cagtagcgag
    34321 ccgtcgctat cgatgccgct tatccccaga atgccgaaat caaggcggaa ctgggagata
    34381 aaatcgagcg ttgcttcgcc aatgatcccg ccatcgcggc tgcgtaattc gccaccggca
    34441 agaatgatac ggaaatcctc ttttaccatc agcgtgttag cgacgttgag attgttggtc
    34501 acaatgcgta aattgctgtg attaagcagc gcgtgcgcca ccgcttccgg cgtggtgccg
    34561 atatcgataa acagcgtcga gccattggga atttgctccg ccactttgcg ggcgatgcgc
    34621 tctttttctt cggtctgggt ggccttgcga tcgtgccacg gcgtattaac cgaactggaa
    34681 ggcagcgccg caccgccatg atggcgcagg atcaggtttt gctctgccag ctcgttgaga
    34741 tcgcggcgaa tagtctgcgg gctaacggag aaatgctcta ccagctcctc ggtgctgaca
    34801 taaccctgct gtttaaccag ttcgataata ccgttgtgac gttgtgtttg tttcattgat
    34861 aaatccctgg aattattttc gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa
    34921 acccacggct aacccggcga tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa
    34981 ccatccggcg acaatccaga tcaacgcaaa gataattaac ccacgttgca ggtaaatgcc
    35041 actttgcgga tcgcgttcgc cacgtagcca gacgtagccc atcagcgcat acaccacgcc
    35101 agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc acatagccgc ttaacagggc
    35161 gctaatgaga gtaatgacaa ttagcttacc gctaccgagg cgtttttcca ccgcaccgcc
    35221 gagataccac caccagagca ggttaaagag gatatgcatc agcgagaagt gcattaacgc
    35281 gtgggtgaag taacgccaga actcaaactt cagcgtcgga tcgaatggcc aggccagcca
    35341 taacatcact tcctgatcgc cgagaatttg catggcgata aacaccacca cgcaggctat
    35401 catcatcacc caggttaccg gacctgcgcg ttcacgcaag gcggcaaaga aaggataacg
    35461 gcgataatgc aggccactgc cggtatggcc cgcctgccag ctggccgcca gataacgcgg
    35521 atctgccggg ttttcgagaa aacgcgccag ctccgcccgc acgcgctcgg cctgggactc
    35581 atccgccagc cagacatcgc tttggttatg ttgttgaatc gtgaggataa caccctgcgt
    35641 cgccatgtaa tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg taatcatcaa
    35701 catcgttgct gtcgcttatt ccacacaaaa ggggacagta taaagcgtta cgcgccgtac
    35761 gccacctctg cgggaaactg gcgctgccag gcttcaaaac cgccatcaat gctgtagacc
    35821 acatcgtagc cctgttgcag cagatactgc gccgcgcctt tgctgctatt gccgtgataa
    35881 cacatcacca tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg
    35941 ttggttaaat ggaaagcctg caccgcatgc cccattgcga aactctgtgg atcgcgaata
    36001 tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat
    36061 tcgaactgat ccatgcgtct ctctttcttt acaaacaagt gggcaaattt accgcacagt
    36121 ttacgtcgaa gcggcagata aacgccataa tgttatacat atcactctaa aatgtttttt
    36181 caatgttacc caaagcgcga ttctttgcta atatgttcga taacgaacat ttatgaactt
    36241 taacgaaagt gaatgagggc agcatggaaa ccaaagatct gattgtgata ggtggcggca
    36301 tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg
    36361 aggcgcagga tctcgcttgc gcgacctctt ccgccagttc aaaactcatt cacggtggcc
    36421 tgcgctacct tgagcactat gaattccgcc tggtcagcga ggcgctggct gaacgtgaag
    36481 tgctactgaa aatggccccg catatcgctt tcccgatgcg ttttcgcctg ccgcatcgtc
    36541 cgcatctacg cccggcgtgg atgattcgca ttggtctgtt tatgtacgat catctgggta
    36601 aacgcaccag cctgccggga tcaaccggtt tgcgttttgg cgcaaactca gtgttaaaac
    36661 cggaaattaa gcgcggattc gaatattctg actgttgggt agacgacgcc cgtctggtac
    36721 tcgccaacgc ccagatggtg gtgcgtaaag gcggtgaagt gcttactcgt actcgtgcca
    36781 cgtctgctcg ccgcgaaaac ggcctgtgga ttgtggaggc ggaagatatc gataccggca
    36841 aaaaatatac atggcaggcg cgcggcctgg ttaatgctac cggcccgtgg gtgaaacaat
    36901 ttttcgacga cgggatgcat ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc
    36961 atattgtggt gccgcgtgtg catacccaga agcaagccta cattctgcaa aacgaagata
    37021 aacgtattgt cttcgtgatc ccgtggatgg acgagttttc catcatcggc accaccgatg
    37081 tggaatacaa aggcgatcca aaagcagtga agattgaaga gagtgaaatc aattacctgt
    37141 tgaaagtgta taacacgcac tttaaaaagc agttaaaccg ggacgatatc gtctggacct
    37201 actccggcgt gcgtccgcta tgtgatgatg agtccgactc gccacaggcc attacccgtg
    37261 attacaccct tgatatccat gatgaagacg gcaaagcccc gctgctgtcg gtattcggcg
    37321 gtaagctgac cacctaccgc aaactggcgg agcacgcgct ggaaaaactg acgccatatt
    37381 atcagggcat tggtccggcg tggacgaaag agagcgtgct gccgggtggt gccattgaag
    37441 gcgaccgtga cgattacgcc gcccgcctgc gccgccgtta cccgttcctg actgaatcgc
    37501 tggcacgtca ctacgcgcgc acttacggca gcaacagcga actgctgctc ggcaatgcag
    37561 gggcgataag cgacctcggt gaagatttcg gtcatgagtt ctacgaagcg gagctgaaat
    37621 atctggtcga ccacgaatgg gtccgccgcg ccgacgatgc cctgtggcgt cgcacgaaac
    37681 aaggcatgtg gctaaatgcg gatcaacaat ctcgtgtgag tcagtggctg gtggaatata
    37741 cgcagcagag gttgtcgctg gcgtcgtaag gatgatgccc ggttgtgaag tgcaaccggg
    37801 ctatttgaat tccttagact attcattgta aataattgaa taacggcccc agagctaaaa
    37861 tcgccaaact taagcacgtt acccaatcgc cccacttctt cctatggctt ctatttttgc
    37921 tacgccactg gcaataatat cccaacgcaa aaccaagctg ggcactaatc gggccgacaa
    37981 aatcagtcca cggctgcggc caaaaagggg aaaaattcag gggaagccag acaaatgtca
    38041 gaaagaaatt tcggatattt tccagcaacg ctgatttctc tggagaaata taaagaatga
    38101 aattcgagag aattaaccag aaatagaccg gagaaaagac cagccagcaa tgtagcctgt
    38161 tttttaattc aggaggcgtt ctgcgggaga attgccaacc gatgccaccc attacactga
    38221 aaaaaagtgc atattcaacc aataactcaa gttgtaattc ctgaagagaa atacgcacaa
    38281 ccaggagcgg aaaggagaat attatttccg ccagaaaagt taacgtgaat ataagtacgg
    38341 ctaaggcaat aaccgtcggc cataccaata atggtacgat aacaaaaccg agaattacag
    38401 ggggaatata aagccggaat ttaatcattt ttattttcct ggtgattcgg atgatgcgtc
    38461 atacatcgcg cataaacact catcaatcat ccagtcgtct tcccagatct gatactgtgt
    38521 gccctgttcg ggctgcttaa gcgggttctc aatcgcctgc cgcgcgcgtt ctttcaggat
    38581 ggattgattg ttgaaggccc aggcattgat ttgggcggta atggctttat cgatatcgtc
    38641 ctggttatct ctgatataac cgtcacgata cgtattcaat aaacaggcaa gagacgctgc
    38701 cgaccaaaat ccgggaactg aaataatggc atcaagcaat ggcgaaagag cgatatcggc
    38761 acggaaaaac atttccagct tgtactgtaa ttcgacagcc tcatccccgg atacccaatc
    38821 agtcatatac tggcaagcaa attgctgcaa tatttcccgt tcatcctcac gccagttttc
    38881 tttactggca aggtttatac gccgtagaga aaatacttcg tgaatattgt caaattgccc
    38941 ctgcgcaaca aactccagta aacgaggcag aaaataacga acctcatcgc tgttttgcgg
    39001 atcaggtcct ggactactat tccaggcatt gattaaggaa taaggaatcg ctcgcaacga
    39061 tgtattccgt aatgcttttt gttcctgttg actcagacaa tattcacaac aaaccacaaa
    39121 ttgcttcggc gccggataac gacgaaaaat atgatatatc tgctcaatta atgcctgcat
    39181 ggcaggagtg gggtgcataa ttaccccctc gcccgctgat cgcgtgtaaa gggttgatag
    39241 acaaaacgag cattcgccgc attcaggcga ataatggcac tacgagagag ggaaaagctg
    39301 ggccgaatgc aataattaca gtcccaacag ctttcctctg tgcaattttc cactgagtaa
    39361 tgacgcgcgt agagtttcag ttcagcctgc ttatcgcgaa atccgcgtaa actgttatcg
    39421 ttttgaaatt gccaggcttc tttaaacact tgaaagatat aaacatcctc aatccaggcg
    39481 tcacattttt taagcggacg ctttgctatg atcacccgct catccgggtt gagcatcgcc
    39541 gtaaattgta cggtgaagtc atttgtcgtg ttcagatcac attgaacatc tggtacatca
    39601 ctgatataga ggtcaaatcc aaagtcagca ccacaggcac aaagacggct aatggtggcg
    39661 gtatcgaggg aaaatcccgg ccatacattg ctggacgtta aaccaatatt taatgtgaca
    39721 tcatatcgag ataatccctc aagaccggca gcaccggcga acttttcccc ttcatccagg
    39781 acctggttaa tgagttgctg aacaatgcct tcatcccagt atgttgattt gcagttggca
    39841 atcgtaatat ccacgccagt gaatggatca gtaccggaga ggcttgcaaa taacgtatca
    39901 actaattcca tttttattat ccggcttaat gcaacatttt attcatcggc acataaccta
    39961 cattttccac caggtttttc ccctgtagaa ttaaaaatat ttttttgttt tacattctgt
    40021 tcatccctat tattgcattt tattttgcgg tgatagctaa accgactctg tactggctca
    40081 aaaattactg atattccact cgcaatatag ccagtgcatt ataacgacct gccgcaggca
    40141 cttttccagt tgtactaaca ggtaaagtca gaaaagtaac atcagcagtt tgcgcagaag
    40201 cagcctggtt ttccaccaat ctgaacggaa cgcctactga tgttgccagg gcatttaaat
    40261 ctacggagtt ttgctgacta tccataatca taataccaac atcaggatta tcttgtgcct
    40321 gaataatact ttgtttccca ttggtatcac gtgtggcgga aatgttaccg tcgaatacca
    40381 acgtcattac cgcatctgta tctttattcg tacaattaat cggaatagtt gttgctacag
    40441 gagtaacacc agcgggcttc gctccggcac cagcccggct caattcactg gcccacacat
    40501 ccggcatatt aacagtaaaa ctcgtaccct cattaatagt gcaaccttgc ggaacctgaa
    40561 tatttaagtt tcctatgtag gcattaaaga cagggatagg accatgttcg gtagggatct
    40621 ggctgacata taatgacacc aataatacgt tacttaatga aacatcagat ataatttttt
    40681 tatcaatcct gattttaata tatccttcag tacctgatgc ccaattagat ggtttgcttc
    40741 tgtcccccgg ataattggta ttataatttt cgaatggcac aattttatct gtcattgtgc
    40801 cattttgatt ataaagtttt atatgagtag agacagatat ttcatgggtg agtggaataa
    40861 atacatcact cccttgatga tcaccactgg caccagacgg atctattcca gagacagcac
    40921 tgaagtaaat ttcataatca tcaagagaat tacaataagc cttataggtt ccaaccttcc
    40981 agatttgtga tggcaataca gtgccaggcg catttttgtt gacatcagaa accactatag
    41041 taccaaaatt ataagaaaag gatgcaggcg tagtattatt actcggtgaa cccggataac
    41101 aat
//
LOCUS       DADTFG010000023        39340 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.023, whole genome shotgun sequence.
ACCESSION   DADTFG010000023 DADTFG010000000
VERSION     DADTFG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.023"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..440)
                     /locus_tag="J1138_001177"
     CDS             complement(<1..440)
                     /locus_tag="J1138_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453005.1"
                     /translation="MGHGTCKAAKSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLLQNNNAIYLFENDNKIELRMGYDSFFSIKTIDKINKENIKEIKLKN
                     DTTYVSLPNPTILITTTSTPERQFSISVLDDDNKENLPTDTAPC"
     gene            complement(1010..2047)
                     /locus_tag="J1138_001178"
     CDS             complement(1010..2047)
                     /locus_tag="J1138_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HBA8453006.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEKVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            complement(2100..2194)
                     /locus_tag="J1138_001179"
     tRNA            complement(2100..2194)
                     /locus_tag="J1138_001179"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2158..2160),aa:Sec,seq:tca)
     gene            2487..3869
                     /locus_tag="J1138_001180"
     CDS             2487..3869
                     /locus_tag="J1138_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBA8453007.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSVTISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3879..6197
                     /gene="yicI"
                     /locus_tag="J1138_001181"
     CDS             3879..6197
                     /gene="yicI"
                     /locus_tag="J1138_001181"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBA8453008.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQMFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGAEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPDADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            6492..8045
                     /locus_tag="J1138_001182"
     CDS             6492..8045
                     /locus_tag="J1138_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA8453009.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEEQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            8072..8542
                     /locus_tag="J1138_001183"
     CDS             8072..8542
                     /locus_tag="J1138_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017801221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HBA8453010.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            8560..8874
                     /locus_tag="J1138_001184"
     CDS             8560..8874
                     /locus_tag="J1138_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBA8453011.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            8899..9990
                     /locus_tag="J1138_001185"
     CDS             8899..9990
                     /locus_tag="J1138_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBA8453012.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            10002..10853
                     /locus_tag="J1138_001186"
     CDS             10002..10853
                     /locus_tag="J1138_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBA8453013.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLVEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            10934..11794
                     /locus_tag="J1138_001187"
     CDS             10934..11794
                     /locus_tag="J1138_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBA8453014.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVSLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMNILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSFGKASLY"
     gene            11842..12774
                     /locus_tag="J1138_001188"
     CDS             11842..12774
                     /locus_tag="J1138_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA8453015.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTSAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            12755..13435
                     /locus_tag="J1138_001189"
     CDS             12755..13435
                     /locus_tag="J1138_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HBA8453016.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(13479..15188)
                     /locus_tag="J1138_001190"
     CDS             complement(13479..15188)
                     /locus_tag="J1138_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBA8453017.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPSWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNHVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(15309..16700)
                     /gene="xanP"
                     /locus_tag="J1138_001191"
     CDS             complement(15309..16700)
                     /gene="xanP"
                     /locus_tag="J1138_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBA8453018.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            16980..18185
                     /gene="gltS"
                     /locus_tag="J1138_001192"
     CDS             16980..18185
                     /gene="gltS"
                     /locus_tag="J1138_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBA8453019.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(18219..20300)
                     /gene="recG"
                     /locus_tag="J1138_001193"
     CDS             complement(18219..20300)
                     /gene="recG"
                     /locus_tag="J1138_001193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBA8453020.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRIDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(20306..20995)
                     /gene="trmH"
                     /locus_tag="J1138_001194"
     CDS             complement(20306..20995)
                     /gene="trmH"
                     /locus_tag="J1138_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBA8453021.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(21002..23110)
                     /gene="spoT"
                     /locus_tag="J1138_001195"
     CDS             complement(21002..23110)
                     /gene="spoT"
                     /locus_tag="J1138_001195"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBA8453022.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(23129..23404)
                     /gene="rpoZ"
                     /locus_tag="J1138_001196"
     CDS             complement(23129..23404)
                     /gene="rpoZ"
                     /locus_tag="J1138_001196"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBA8453023.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(23459..24082)
                     /gene="gmk"
                     /locus_tag="J1138_001197"
     CDS             complement(23459..24082)
                     /gene="gmk"
                     /locus_tag="J1138_001197"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBA8453024.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            24340..26022
                     /gene="ligB"
                     /locus_tag="J1138_001198"
     CDS             24340..26022
                     /gene="ligB"
                     /locus_tag="J1138_001198"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBA8453025.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(26019..26636)
                     /locus_tag="J1138_001199"
     CDS             complement(26019..26636)
                     /locus_tag="J1138_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBA8453026.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(26888..27751)
                     /locus_tag="J1138_001200"
     CDS             complement(26888..27751)
                     /locus_tag="J1138_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBA8453027.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            27878..28594
                     /gene="rph"
                     /locus_tag="J1138_001201"
     CDS             27878..28594
                     /gene="rph"
                     /locus_tag="J1138_001201"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBA8453028.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALNKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            28660..29301
                     /gene="pyrE"
                     /locus_tag="J1138_001202"
     CDS             28660..29301
                     /gene="pyrE"
                     /locus_tag="J1138_001202"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBA8453029.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(29338..29934)
                     /gene="slmA"
                     /locus_tag="J1138_001203"
     CDS             complement(29338..29934)
                     /gene="slmA"
                     /locus_tag="J1138_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBA8453030.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(30041..30499)
                     /gene="dut"
                     /locus_tag="J1138_001204"
     CDS             complement(30041..30499)
                     /gene="dut"
                     /locus_tag="J1138_001204"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBA8453031.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(30477..31697)
                     /gene="coaBC"
                     /locus_tag="J1138_001205"
     CDS             complement(30477..31697)
                     /gene="coaBC"
                     /locus_tag="J1138_001205"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBA8453032.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRIAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVASEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            31869..32537
                     /locus_tag="J1138_001206"
     CDS             31869..32537
                     /locus_tag="J1138_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBA8453033.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            32754..32990
                     /gene="rpmB"
                     /locus_tag="J1138_001207"
     CDS             32754..32990
                     /gene="rpmB"
                     /locus_tag="J1138_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBA8453034.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            33011..33178
                     /gene="rpmG"
                     /locus_tag="J1138_001208"
     CDS             33011..33178
                     /gene="rpmG"
                     /locus_tag="J1138_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBA8453035.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            33276..34085
                     /gene="mutM"
                     /locus_tag="J1138_001209"
     CDS             33276..34085
                     /gene="mutM"
                     /locus_tag="J1138_001209"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBA8453036.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(34124..34603)
                     /gene="coaD"
                     /locus_tag="J1138_001210"
     CDS             complement(34124..34603)
                     /gene="coaD"
                     /locus_tag="J1138_001210"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBA8453037.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRASQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLENVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(34611..35888)
                     /gene="waaA"
                     /locus_tag="J1138_001211"
     CDS             complement(34611..35888)
                     /gene="waaA"
                     /locus_tag="J1138_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HBA8453038.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            36301..37359
                     /gene="rfaQ"
                     /locus_tag="J1138_001212"
     CDS             36301..37359
                     /gene="rfaQ"
                     /locus_tag="J1138_001212"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBA8453039.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            37356..38480
                     /locus_tag="J1138_001213"
     CDS             37356..38480
                     /locus_tag="J1138_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA8453040.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            38473..39270
                     /gene="rfaP"
                     /locus_tag="J1138_001214"
     CDS             38473..39270
                     /gene="rfaP"
                     /locus_tag="J1138_001214"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBA8453041.1"
                     /translation="MVELKEPFATLWRGKDPFGEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEVLKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEEKATKIRERTIRKSL"
BASE COUNT         9457 a         9516 c        10444 g         9923 t
ORIGIN      
        1 aaacaaggtg cggtatcggt aggtagattt tctttgttat catcatccag aacagaaata
       61 gaaaactgtc gttcaggtgt tgatgtcgtg gttattaaaa tggttggatt aggaagagaa
      121 acatatgttg tatcattttt tagttttatt tctttgatgt tttctttgtt aattttatca
      181 atcgttttaa tagagaaaaa agaatcatac cccattcgca gttcaatttt attatcgttt
      241 tcgaataaat aaatggcatt attgttttga agcagattga atatattgtt agcgcaagtt
      301 tgagttgcta gccaggatgc cgctggatga ctcaaattaa tatgcgtgct aaagactttg
      361 acgcgactac ttctgttttt ctctttactt tcaattaaat gcctagcgct tttggctgct
      421 ttacaagttc catgccccac acggcaaaaa taatctcctg aaaggatact tacaatcagt
      481 attacaccaa tgacgacaga aaaaatagtt ccgatgagtt tgatgatgac aatccttgtg
      541 cagtgttatt ttcaccatgg attataatgt tatagtgaaa ttattgcaca tgtaattttt
      601 ctttgattgt tttaacatca catttatgca cttcttcatc tttgtagcag gtaaaagcca
      661 tatatggatg gctggtgtcc ggtgcagata aaatgcaact tattcttgat agcttgaaaa
      721 aaagagaggt ttttttgagt gacggttgat agtcggtggc aaggaagacg ttttcgtaca
      781 ctcggcaaga aaaacgaatc actctgtcat actaccagat acatcagcag ggtcaggttg
      841 ctcagaaata agcaggatgg ggatcactga agaggcattt tggcgatcac aggagtcgca
      901 tttgatggtt aagttattga tttaatgttt atattttgaa tttgataaat gagtaacccc
      961 ataagtaccc ccaaatggtt ttgcgtatgg cgtttggggg gattccgggc tactttttcg
     1021 actctttatt ccactgtagc aaaccagcga tatccttctc gccttcggct tcttttaagc
     1081 gccgttgttg ttcggcaaac tgaacatact ccgcctgcgc tttttcatcg gccattttct
     1141 tgctgatttt acccgcgccc tgtagaacct ctcggtcgtt aaattgcagg aactgatcca
     1201 gcttatcctg ccagtcgcgc aaaaagactt gctgacgacg acgggcctga tcctcggcaa
     1261 agtccagcca catattaacc acgcgattaa gttcgctgac ctcatcctgg gtgagataat
     1321 tttttgccac cgtcacgtcg cccttacgta ccttttcacc tttataactg gtcaagccca
     1381 tgtgcggctg gctggcatct gctcgtttgt ggatgagttc cgcagcggta tgcccggtac
     1441 aggcaaaatg cagtttattc tggatggttt gaaaaaattg cgtggtttct ttaagcgatg
     1501 gctgatagtc ggcagctaaa gcaaagatct cccgcacccg caaatatacc cgacgctcgc
     1561 tggcgcgaat atcgcggatg cgttcaagca tctcatcaaa atagtcgggc acaaccgatg
     1621 aaccgacagg cggattcttc agccgctcat cgtccatcac aaaacctttg atcaggtatt
     1681 cctgaagcgt ctgggtggcc cactggcgga attgcgtgcc tcgaggcgag cgaacgcgat
     1741 agccgacagc gagaatggct tcgaggttgt aatggtctat ctctctggaa acctgacgtt
     1801 ttccttcctg gcgaactatc cggaatttcc ggatggttga gttttgaaca agttcacctt
     1861 cggaaaatat attgattaaa tgttcgttaa tagtgcgaac atctttatcg tacagttcag
     1921 ccattgccgc ctgggataac cagattgtgt ctgactcaaa gcgacattca acacgcactc
     1981 ttccgtcacc gctggcaaac ataataaact cgcctgctgg gggctgggtt aagtctttgt
     2041 ctgacatgtc gcctccgaag gagtgttgtt gacgtcggga gagttcagga gaggcatttt
     2101 ggcggaagat cacaggagtc gaacctgccc gggaccgctg gcggccccaa ctggatttga
     2161 agtccagccg cctcaccgga gacgacgatc ttccgcgcct cgattgctac atggaggcgg
     2221 ggcgcattat agctacttcc ttgagtttct acatccccca gatcagtttt ttccgcctaa
     2281 tacacgcagc gtattatcac gaaagcatta tagttcatat aaaaatcata ttttacaatg
     2341 atatatgagc gaaattaacg ctaccacgat cacataaaac aagaatcata aattaataac
     2401 cagatatcgg aatattcgct ctcgcaggga tggtttacaa aatgcgttat cggtctcagc
     2461 acccctatag catcaaggaa aagcagatga agagtgaagt gttgtccgtt aaagagaaaa
     2521 ttggttatgg catgggagac gccgccagcc acattatttt cgataacgta atgttatata
     2581 tgatgttctt ttataccgat atttttggca ttcctgccgg atttgtcgga accatgtttt
     2641 tggtcgctcg tgcactggat gcgatttccg atccttgcat ggggttgttg gccgatcgaa
     2701 cgcgctctcg ctggggtaaa tttcggccgt gggtgctgtt tggcgcactg ccattcggga
     2761 tcgtctgtgt actggcctat agcacgccag atctcagtat gaacggaaaa atgatttatg
     2821 cagcaattac ttacacccta cttaccttac tttataccat cgtcaatatc ccttactgcg
     2881 cattgggtgg tgtaatcacc aatgacccga ctcagcgtat ctcgctgcaa tcctggcgtt
     2941 ttgtgctggc gaccgcggga ggcatgctct ctactgttct gatgatgcca ctggttaatt
     3001 taattggtgg tgataataaa ccactcggtt tccagggcgg tatcgcggtc ctttccgtgg
     3061 tggcatttat gatgctggca ttctgtttct tcaccactaa agaacgcgtt gaagcaccac
     3121 ctacaacgac gtctatgcgg gatgatttac gtgatatctg gcaaaacgac cagtggcgta
     3181 ttgtcggttt cctgacaatt ttcaatatcc tggcggtgtg cgtacgcggt ggggcgatga
     3241 tgtattacgt cacatggatt ttgggtacgc cggaagtgtt tgtcgctttt ctcaccactt
     3301 attgcgtggg taacctgatt ggctccgcac tggcaaaacc gctgaccgac tggaaatgta
     3361 aagtcactat cttctggtgg acgaacgccc tgctggcagt gattagcctc gcgatgttct
     3421 ttgttcctat gcaggccagc atcaccatgt ttgtcttcat cttcgtgatt ggtgtgttgc
     3481 atcaactggt gacacctatc cagtgggtaa tgatgtccga taccgtcgac tacggcgagt
     3541 ggtgtaatgg taaacgcctg accgggatca gttttgctgg cacgctgttt gtgctcaaac
     3601 tggggttggc cttcggtggt gcccttatcg gctggatgct ggcttatggc ggatatgatg
     3661 cggcagaaaa agcgcagaac agcgtcacaa ttagcatcat tatcgcgcta ttcacgattg
     3721 ttccggcgat ctgttacttg ctgagcgcga ttatcgctaa acgctactac tcgctcacga
     3781 cgcacaatct gaaaaccgtt atggaacagc tggcgcaggg caaacgccgt tgccagcaac
     3841 aattcacctc tcaagaagtg cagaactaag gaacggcaat gaaaattagc gacggaaact
     3901 ggttgattca acctggcctc aatttgattc acccgcttca gatgttcgag gttgaacagc
     3961 agggtaatga aatggtggtc tatgctgccc ctcgtgatgt gcgtgaacgt acctggcagc
     4021 ttgatacgcc tttatttacg ctgcgctttt tctccccaca ggaaggtatt gtcggtgtac
     4081 ggattgagca ttttcagggg gcgctgaata acggtcctca ttacccgctc aatattttgc
     4141 aggacgtgaa ggtcactatc gaaaacacag aacgttacgc tgagtttaaa agtggcaact
     4201 taagtgcgcg tgtcagcaaa ggtgagttct ggtcactgga ttttctgcgc aacggcgaac
     4261 gtattaccgg tagtcaggtg aaaaataacg gctacgtgca ggacacgaat aatcaacgaa
     4321 attatatgtt tgagcggctg gatcttggcg ttggcgaaac agtttacggt ctgggagagc
     4381 gctttactgc cctggtgcgc aatggccaga cggtagagac ctggaaccgg gacggcggca
     4441 caagtactga acaggcgtat aaaaatatcc cgttttatat gactaaccgt ggttatgggg
     4501 tactggtcaa tcatcctcaa tgcgtctctt ttgaagtggg agcagagaaa gtctccaaag
     4561 tacagttcag cgttgagagt gaatatctcg aatactttgt tatcgacggc ccgacgccga
     4621 aagcggtact tgatcgttat acccgtttta ctggccgtcc ggcgctgccg cccgcgtggt
     4681 ccttcggtct gtggctaacc acttcattta ccaccaacta cgacgaagcg acggtaaaca
     4741 gctttatcga tggtatggcg gaacgcaatc tgccgctgca tgttttccac tttgactgtt
     4801 tctggatgaa agccttccag tggtgcgatt ttgagtggga cccgctgact tttccggacc
     4861 cggaggggat gatccgccgt ctgaaagcga aagggctgaa aatctgcgtc tggattaacc
     4921 cctatattgg gcaaaaatcc cccgtcttta aagagttaca agagaaaggt tatttactca
     4981 aacgcccgga cggttcgctg tggcagtggg ataaatggca gccaggtctg gcgatttatg
     5041 actttaccaa tccggatgcc tgcaaatggt acgccgacaa actgaaaggt ctggtcgcga
     5101 tgggcgttga ttgctttaag accgactttg gcgaacgtat tccaaccgat gttcagtggt
     5161 ttgatggttc cgatccgcag aaaatgcata accattatgc gtacatctac aacgaactgg
     5221 tgtggaacgt gctcaaggac accgttggtg aggaagaagc cgtcttgttt gcccgctcgg
     5281 cctccgttgg tgcgcagaaa tttccggtac actggggtgg cgactgttac gctaactacg
     5341 aatcaatggc ggaaagcctg cgcggtggtt tgtctattgg cctttcaggt tttggcttct
     5401 ggagccacga tatcggcggc tttgaaaata ccgctccggc gcacgtttac aaacgctggt
     5461 gcgcgtttgg tttgctttcc agccatagcc gtttacatgg cagcaaatct tatcgtgtgc
     5521 cgtgggccta cgatgatgag tcctgtgatg tggtgcgctt cttcacgcaa ctgaaatgcc
     5581 gcatgatgcc gtatctgtat cgtgaagctg cacgtgcaaa cgcccggggt acgccgatga
     5641 tgcgggccat gatgatggag ttcccggacg atccggcttg tgattacctt gaccgtcaat
     5701 atatgttagg cgacaacgtg atggttgcgc ctgtgttcac agaagcgggc gatgtgcagt
     5761 tctacctgcc ggaaggtcgc tggacacacc tgtggcacaa cgatgaactc gacggtagtc
     5821 gctggcataa acagcagcac agcttcctga gtctgcccgt ttatgtgcgt gatagcaccc
     5881 tactggcgct gggcaacaac gatcaacgtc ccgattacgc gtggcacgaa ggtacggcat
     5941 tccacctctt taatctgcaa gacgggcatg aagccgtctg tgaagtgccc gatgctgacg
     6001 gttcggtgat ctttacttta aaagcagcac gtactggcaa cacgattact gtgactggta
     6061 cgggcgaggc gaagaactgg acactgtgct tgcgcaatat tgtgaaagta aatggtctgc
     6121 aaggcggttc gcaggctgaa agtgagcagg gactggtggt gactcctcaa gggaatgcgc
     6181 tgacaattac gttgtaatct attgaagaat gccggatacg aatggttttg tgtcgtatcc
     6241 ggtaaataat ctcacatctg gtaataaata tttcttccat cgtatttgcg agcatgatca
     6301 cgattattca tcacgaaaat gtgatctgtg ctgaaaaata tcccttaacc gccttgcttt
     6361 gttttatcac tgtccggatt acggacagat tttagtattg ttgtgaaatt attctgcttt
     6421 atatttaccc tcagagatgg caactgaagc tcttccatat ggggcttctt aatctgtgat
     6481 aagggtaaaa aatgcaaatg attacctcgc gacaaaacag attattacga ttccttttac
     6541 ctcgaaggga atatacgact attgttacga ttgccggtta tttaaatgtt tcggaaaaaa
     6601 ccattcaacg tgatttacgt ttacttgagc aatggctggg acaatggaga ataaatgttg
     6661 agaagcgtgc tggcgcgggt gtgatgttaa gtgcggagaa tattgctgat ttgctgcatc
     6721 ttgatcattt gttggtggca gaatgtgaag agattgatgg tgtaatgaat aatgccaggc
     6781 gtgttaaaat agcgtcgcag ttattaagtg aaacaccgaa tgaaacgtcg atcagtaaat
     6841 tgtcagaacg ctactttatc agcggagcct ctattgttaa tgacctgaga gtaattgagt
     6901 cctggcttgc gccgttgggg ttatcgttga tccgcagtcc aagtggtacg catattgaag
     6961 gtagtgaagg gcaagtccga caggcaatgg cattactgat taacggcatt attaaccata
     7021 atgagccgca aggtgtcgtg tattcacgtc tggatcccgg aagctataaa gcattagtcc
     7081 attattttgg agaagaagag gtgttatttg tccagtcatt gttactggat atggaaaatg
     7141 aattaagttg gtctttggga gaaccttatt acgttaacat ttttactcat atccttatta
     7201 tgatgtatcg caacacgcac gggaatgcgt tatcaagaga agaagaacaa accaggcaat
     7261 atgatgaaaa tatctttaat gttgccagtc agatgattca taagatagaa caacgaattg
     7321 cacatacatt gcccgatgat gaagtctggt ttatttatca atatatcatt tcatcaggtg
     7381 tggcgattga tggacaaaaa gatgttagca ttatttcaca tatgcaggcc agcaatgaag
     7441 cgcgtctgat tacctggcgt ttaattacgg tattcagtga catcgtggac tgcgatttta
     7501 gtgaagacag cgcattatat gatggcttat tggtgcatat taagccgctg attaaccgtc
     7561 taaattatcg cattcatatc cgtaatccat tgctggaaga tattaaagca gaattagcgg
     7621 atgtctggcg gttgacgcaa tatgtggtga atcaggtatt taaaacctgg ggtgagaatg
     7681 cagtgagcga ggatgaagtg ggttacctga ccgttcattt tcaggctgcg atggagcggc
     7741 aaattgcccg taaacgtgta ttactggtct gttcaaccgg aatcggaact tcgcatctac
     7801 tgaaaagccg tattctgcga gcatttcctg aatggacgat tgttgatgtt atttcagcag
     7861 cgaatttatc acaggttttg cctgacaata tcgaactgat tatttcgacc attaatttgc
     7921 ctacagtcac tatgccggtc gcttatgtta ccgctttttt taatgatgcc gatattaagc
     7981 gggtcactga aatggtgatt acggaaaaat tacatcatgc gacgtctcgg gtcgttgaaa
     8041 tttaatgtca gaattttatg aggtatgacg aatggacata acaaaaatcc tgaatacgaa
     8101 tcgcgttatt ttagatatgc aagcaacgaa taaagcggag gcgattgaag aattaactaa
     8161 tcttctcaaa aaagatggtg cgatcgattg tcgggaaacc tttattaaag atgtctggca
     8221 gcgcgaagcg gaaggctcga ctggttttga aaatcatatt gcgatcccgc atggtaaatc
     8281 ttctgcggta attaatacta cgctggcaat tggccgtacc cgccaggata ttccctggga
     8341 aacgctggat ggcagtagcg tgcgctgtat tattttattt gcggtacgtc ttgaagatca
     8401 aaataccacc catatccgtt tactttccca ggtcgcgggc gcgctggcgg atgatgacat
     8461 catagaacaa ttgttagttg agacgagtcc gcaaaaaatc attgatctct ttagtcagta
     8521 tgcacaatcc gatgtctgct aaataacagg aagtatgtca tgaatattgt ctgtgttgcc
     8581 gcctgtacgg cgggtattgc tcatacctat attgctcgtg aaaagttaat caaaggagca
     8641 aaagctctgg gccataccat taaagttgaa acccagggaa ccattggtac agaaaatgaa
     8701 ttacaggcgg cagatatctc tgctgctgac gtcgtgattc ttgctgtaga tgtgaaaata
     8761 aaaggagaag aacgcttcac caataaacgt attgtgcgag taaagacaga gatcgtcatt
     8821 aaatcgcccg ttcaatttct tgaaaaggtc gaaaaatcac tgggaaacaa atgatcctta
     8881 tactggaggg atcttaatat gaaagaaaat aaaattccgg tttcacagga aattaaaaaa
     8941 catttattaa ccggcatctc gtggatgata ccccttattg tggccgccgg gatatgtatt
     9001 gcgttggggc aagttcttgg cggtacagat gttggtgaaa aaacaggaac aatcccgtgg
     9061 atgcttaatc agattggcgg ctggggaatg gggctgattg tgccgctgat tagcgcggca
     9121 attgcctatt ctattgccga tcgtcccgga tttgccccgg gcctgattgt cggctttctc
     9181 tgcgggcaaa tccataccgg atttattggc ggtatgttgg gtggtttcct ggtgggctac
     9241 accattttgc tacttaaacg ctatatccgc ctgccgcagt cgatgcaagg actcatgcca
     9301 atcatggtgt tgccggtatt aagcaccatt atcggtgggc tgttaatgat gacgctgatt
     9361 ggtaaaccca tcgcctggtt acaagaagca ctgattcatt tgctcgagtc gatgcagggc
     9421 ggatcgcgct tcctgatggg ggccatactt ggcgcgatgg caaccttcga tttcggtggt
     9481 ccggtcaaca aaaccatgtc gctgttttcc gatggcttgc tggtcagtgg cgtctacgga
     9541 ccagaagccg tgaaatttgt cggctcaatc atccctccgt ttggcatcac actttcgttc
     9601 ttactgacac gccataaata cacccgtgcg gagcgcgagg cgctgaaagc cgcgtttccg
     9661 atggggatct gcatgatcac tgaaggagtc attccgatcg ccgcgcgtga cctgctgcga
     9721 gtagtgggat cttgtgtggt ggcatcggcg gtcgcgggcg gcttaatcat ggtgtggggc
     9781 gtggagagtc cggttccgca tggcggcatg tttgttgtgc ctctgttcac ccatcctctg
     9841 ctgttttgcc tggcgctggg tataggcacg gtgatttgtg gcgtgatgct ttcactctgg
     9901 aagaaaccgg taacggaacg cgacgaagag tttgacgaac tgagcgatca aaagctgaaa
     9961 gatgaagaga tcactttcac cctcgaataa ccggaggccc tatgtttgcc gatatgaaaa
    10021 gtatggtgat gaaagcctgg catgaacatt acgccttgct ggcgatcaac tgtatgaatc
    10081 tggaaagtgc tcacgcagct atccgcgtgg cggagaagaa tcgcgctccg atcattctca
    10141 atctctatca ggggcatctg gcgcattttc ctgcacccgt cgcggcggca gtggttaaaa
    10201 cgctcgcgga atctgcctca gtaccggtca ctctggcgct tgatcatggc aaaaatccgg
    10261 actgtattcg tcaggcgttt cgtgctggtt tcagcggatt aatgattgat gcttctgctt
    10321 ttcccctgga ggaaaacgtg cgccagacca gggcggtggt ggaactcgcc gccagcgcgc
    10381 agctctgcgt ggaaggcgaa ctgggccatc tggcagatgc gccgcgttac gaccaggccg
    10441 ccaatgcaga cctgatgacg caacccactg atgtggaacc gtttatcgcg cagacaggca
    10501 ttgatctact ggccgtctcg gtaggcacgg cgcacggcat gtacgcgcct ggtgttgtac
    10561 ctgctattga ttttcagcgt ctggtggaga ttttccactg ctcgagcgtg cctctggcat
    10621 tacatggcgg tagcggtacg ccgttcgatc agctccagct ctgtacgacg ctgggggtag
    10681 caaaaatcaa cgtgggggcg gccatttttg aacgggggaa atccgcgctc ctgcataccc
    10741 tctgtcacga tatatccatt gaactggtgg acgcgctgaa agctatggaa ctggccttcg
    10801 aagaagcgat tacaccgtat ttacaggcga gcggctctat cggcaaagcc tgattcattt
    10861 tctgtcatgg cctttatgcc agtgcaataa cgcaccggca gggcggacta cacgctgaaa
    10921 ctggagattt aacatgttag tttcgatgaa agaattactg caacctaccc gccagcacgg
    10981 ctttgccatt ggcgcattta atgtggcgga taactgtttc cttcgcgcag tcgtggatga
    11041 agcagaagcg acaaatactc cggcgattat tgccatccat cccagcgaac atgattttgt
    11101 gggcgatgcc tttttcgctc acgttcgcga aattacccag cgtagtccgg tcccgtttgt
    11161 actgcacctc gatcatggtg cctctgtaga gcatgttttg cgtgcgattc gctgcggatt
    11221 tacctcagta atgatcgacg gttcgctgct gccgtatgaa gaaaacgtgg cgctgaccgc
    11281 agaagtggtt agcctcgctc acgcggttgg cgtgtcggtt gagggtgaac tgggaaccat
    11341 cggacaaaca ggaacgtcgg tagaaggtgg tgtttcacaa gtgacctaca cagatccggc
    11401 gcaggccgcc gattttattg ctcgtacagg tgccgacaca ctggctgtgg cgattggtac
    11461 agcgcatggc atctatccaa aagggatgca gccgaagctg caaatgaaca ttttgcgcga
    11521 cattgccgga cgtattgata ttccgctggt gctacacggt ggttcggcaa acccggacgc
    11581 ggagattgct gaatcagtta cgctcggcgt cggcaaaatc aatatctcca gcgatatgaa
    11641 atacgcctat ttccagaaag tgcgtgaaat tctcgctaaa gagagctggt gggacccgaa
    11701 cgtcatctat ccggatgcga ttaacgcggc gcgggaagtc attcgccaca aaatgaaact
    11761 gttcggttcg ttcggtaaag catcactgta ctaagccaga agggcggggt gacccgcctt
    11821 ttacgcaaca aggaacgaga tatgcactat ctggggttgg atattggtgg gaccaaaatc
    11881 gccgccgttg tcatggatgc gcatggctgg gagattcgcc gttaccgctg cccgacgcaa
    11941 aagtcaacat atcaacaatt tgtctcatgc gttgtggcgc ttatcgagca gattaggcgg
    12001 gacgttcaac gaccgatgct gacagggatc gccttacccg gcagtatctc gccactcact
    12061 ggcctgatta aaaacgcgaa tattcaggtg attaacggtc atgcgttaca ggctgatttg
    12121 cagcaattgc ttgggcaacc ggtggtgata gccaatgatg gtaactgttt cgcgctatca
    12181 gaagcttgcg acggtgccgg gcaagattat gacgtggtat ttggtattac gcttggtacg
    12241 ggctgcggcg gtggcattgc catcaagcaa cgaccgttta taggggcctg gggaaatgct
    12301 gccgaatgcg gtcatatcac gttgccaggc tatacggagc aggaagatgg tccatcagtc
    12361 agttgctatt gcggcaaaca caactgcgtg gagtcgtttg tttccggcag cggttttagt
    12421 gaacgctatc aacagatgac tggtaacttg ctcacttctg cggcgattgt caccctggca
    12481 caacgtggtg atgcttgtgc catgcagcag gtggcacgtt ttcgccaaca gcttgcccgc
    12541 acgctggcaa ccatcgttaa tgttgttgac cctggcgtga ttgtgatcgg cggcgggctt
    12601 tcgaatgtgg aactgcttat caccgatctg aacgcagaag tcgctccact ggttttcacc
    12661 gatcaattca ccactcccat tgtaaaagca cagcacggcg acagtagcgg aatgcgtggc
    12721 gctgcctggc ttgctatgcg caacggagaa gccaatgaaa cgttcacaaa ttaattatgt
    12781 tattgataaa gcacatgcca ttgcgcagac cttccgcgtt tgtttgccag agttcgccta
    12841 ttttacggtg gatgactggc tgcagcggga acgggataac tggcaagaag tggtcgactt
    12901 acagctcggt tgggatatca ccgattttgg tcgtggtgat ttcaaccaaa cgggactaac
    12961 actgctaacg atgcgcaacg gcgcgctggg gtcggcggca tatccaaaac cgtatgctga
    13021 gaaaatgttg cagatccaac aagatcagca gactccctgg cattttcaca cccacaaaat
    13081 ggaagatatc ctcaatcggg gcggtggaga cttatgcatg cagttggcat gggccaacga
    13141 agccaatctc tgtgatgatc aacgagtcat taccgtgagt gttgatggtc agcggcgtac
    13201 catgaaacct ggtgaaacgc tggtgctaaa gccggggcag ggcatttgcc tgccaccgcg
    13261 tctgtatcat cgcttctggg cggaaaaagc gtttgttctc ggctgggaaa tctcgatggt
    13321 aaatgatgac caacacgata attattttct tgagccggga ggacgttttc cggcgataga
    13381 agaagatgaa ccggtgaaat ggttattgtg cagtgagtat ggaattttgc gctaataacg
    13441 gggaacgcta gcgctgatat ccgctagcgt ccttattttt actgcaccgg ctccgtcgac
    13501 gtaacttcac cactggaaac tacgcccgca ttcatggttt gagtcacttg ctgctttgcg
    13561 gcattcacgg catgcaattg cccgctaacc gtaggtttca gcgggacatt gacctgaata
    13621 tcgccactgg cggtaagctg aatattgccg tcgccagtca acggtaattc aggccatccc
    13681 caatgttgca aaatattcac cggcacaccg cgtccattca ggctgatatg ggtctgacgt
    13741 tgtggcgttt gtgaaacact ggcggtggct tccagaatgc ctttttcagt aaatgcactc
    13801 agttcgctga tattcaccgt gctgctgttg gcagttagcg ccagcgacgg acgacggaca
    13861 tcaacatgat taaacgttgc ggcggcggca ttcagattcg ccgagccact ccagacgccc
    13921 catttatgat cggtaaccag cgtcagattg gcaccgtaac catcgagcgc ggtgagctgc
    13981 cacgggaagt cagggtcgat atcaatgatc agattgcggc tggcgctaaa tctcttcagt
    14041 tgcaggctgt ttaaccaact gggtgtcgtt tccatccaca actgttgcca gttttttggc
    14101 aaggtatatt ccagtccggc aattgccgca tcatcgagga tcagcgtttt cccgtcgcgt
    14161 tgccagtttc ctgacgttct gaccataccc ccttcccagc ggctggtgaa ctggcgcagt
    14221 gctacgccct gcggggaaaa ttccgcgttt ataatcggat caaataaatg cagcgagcca
    14281 taaataaact cgctggcgtt catcgacagt ttgccttcct gtgtctgcca gtcatcttta
    14341 ctgaaggtca tgttgcgcaa gctgagatcg aggtcggtca ccgcccagtc cggaccttgc
    14401 aaacgagcgt cgatgacttc caggcgacca atctgcaacg acgggacaga gcgtaatggc
    14461 gcaaagaagt cggttagcga tttttcgctt tgtagacgga tatccgccat gcgcaggttt
    14521 tccacttgcc agctgccgtc agcgttacgc tgcgcgtttc cggttaatgt cccgcgggcg
    14581 atgtcggcac ccaggttagt cagcgtaacg cgatcgttat caatactgcc ttcaatcagt
    14641 acattggtgg caggaacatc attgagcgaa agcgatccgg cactaaactg aatctgcgcc
    14701 ttcgtaccca gcactttacc ggcttctggt gaccacggaa ccacgccgcc atttacccgc
    14761 tgcgcgctca gtttccattc gctattcggg ctattaaacg ccatatcacg cagttgcaga
    14821 cgatcggctt tgaacggtag cggcgcggtc tggtcggtga gattcagcgt cccgttttcc
    14881 agcaggatgg tatcgacatg gcgtggttcg gtcagttgcc gactgcttag cgcaatgtcg
    14941 acactttttg ctaccagggt cgcgggctgg ccatcacggc caaacgtgac gttctccagc
    15001 acgatatgag atggcgcgga aaaacggtga tccatcgccc cgaaggccag atgatagtcg
    15061 ctattctcgg aaacccatgc gctgatatgt tctgctcccc agcgagtttg cagaagaaaa
    15121 tagaggccag cgatcaccac taacagagcg atgagaatgt agagaagcag cttcccaata
    15181 aatttcatgg tcttccatcc cgtgaaatgc acataaggga gttatgcacg atttacgcgc
    15241 aatcctcaag gcgggaatgg tgaaagaggt gagactgcgg cggtaattat cttaccgccg
    15301 ccagtgaatt actgtttttc tggtgggaaa atcagattca gcacgatggc agtaataccg
    15361 cccgcggcaa tcccggagga gagcaggttt ttcagccatt caggagcaaa ctgcaaaatc
    15421 agcggctgct gagacacgcc cagaccgacc gccagcgaca gcgcgataat cagaatcgcc
    15481 cggcggttca gcggctcacg agaaacgata cgcacaccgg aggcggcgat ggtgccaaac
    15541 attacaagcg ttgcgccgcc cagaaccggt tctggaatgt gttgtacaaa accgctcact
    15601 gccgggaaca gacccagcac gatcaacatc agcgcgacga caaaaccgac atagcggctg
    15661 gcaacaccgg ttaactggat caccccgttg ttctgcccga agcaggagtt cgggaaggta
    15721 ttaaataccg ccgagacaaa cgagttcagg ccgtttgcca gtacgccgcc tttcaggcgt
    15781 ttcatgtaca gcggaccgga aaccggctgt tcggaaacgt cagaggtcgc cgtgatatcg
    15841 ccaatggttt ccagcgaagt gatcataaag accagcatca gcggcagcag cagactccat
    15901 tcaatgccaa gaccgtaata gagcggcgtt ggcaccataa tcagttcttg tgtcatcggt
    15961 tcgttgcttt ctggcaacat gcccataaac cacgccagcg catatccggc cgccatcgca
    16021 atcaccagtg aggccacgcg taagtaaggg ttacgttgac ggttaagcag gataattaag
    16081 gctaagacca cgcctgccag cagcagattt ttcggtgcgc cgaaggtgtt atcgctcatg
    16141 gctgcgtaac cgccgccaat ggaggtcagc ccaacctgaa ttagcgacag gccgataatc
    16201 atcaccacaa cgccagaaac cagcggcgta ataatgcggc gcgccagatg cagaacgcgg
    16261 gagatcacca tctcggtaca acttgccagc atcaacgtgc cgaacaaagc tgccatcatg
    16321 gtaggaacat cagcaccacc ggtcttcagc gctgtgccgc ccataatcag cggagcaaca
    16381 aagttgaagc tggtgccctg aatagacaac aacccggagc caaccggacc ccaggcctta
    16441 atttgaataa tcgatgccac accggaggca aacagcgaca tactaataat gtgttgcgtg
    16501 tcttgtgccg gtaaacccag cgcctggcag attaatagcg ctggcgtgat caccgcaacg
    16561 aacatcgcca gcaaatgctg acaggcggca aacagggttt gaggaagcgg cggacgatct
    16621 tcaagacggt aaatcagttc gctgttttga gtctgcgcaa ccggttgcgc attttctgac
    16681 tcgagggtgg aaacagacat cgaaaacaat cccgtggtag caaagcgggc attttagctg
    16741 accgaaagag aaaagcaaac gtttgccaca tcctcatcta aatatttaaa tgagataaat
    16801 gtactttttt atataacttt tagttgtttt ctgcttaaaa tccatgccat ttttctagtt
    16861 tcagctaaga attcgaacca ggaaagcatg actcgatact cgtcatattt cgccttactg
    16921 caaatgcgcg atgaagtatg acgagtatga aagagtgatg cggacacaaa ggagtaacta
    16981 tgtttcatct cgatacttta gcaacgcttg ttgccgcaac gctgacgttg ctgctcgggc
    17041 gcaagttggt ccattccgtc tcctttttga aaaaatacac cataccggaa cctgttgcgg
    17101 gtggtttatt agtggcgctg gcgctgctga tactgaaaaa aagcatgggc tgggaagtca
    17161 actttgatat gtccctgcgc gatccgttga tgctggcttt cttcgccacc attggcctga
    17221 acgccaacct tgccagtctg cgagctggtg ggcgtgtggt gggggttttc ctgattgtgg
    17281 ttgttggcct gttggtgatg caaaatgcca ttggcattgg tatggctagc ctgttagggc
    17341 ttgatccgct gatggggctg ctggccggtt ctattacgct ttccggcggt cacggtacgg
    17401 gtgctgcatg gagtaaattg ttcattgaac gttatggctt caccaacgcg acagaagtgg
    17461 cgatggcctg tgcaacgttc ggtttggtgc tgggcggctt gattggcggt ccggtggcgc
    17521 gctatctggt gaaacactcc accacaccga acggtattcc ggatgaccag gaagttccga
    17581 ctgcgttcga aaagccggat gtgggccgca tgatcacctc gctggtgctg attgaaacta
    17641 tcgcgctgat tgctatctgc ctgacagtgg ggaaaattgt tgcgcaactt ttggctggta
    17701 ctgcttttga actaccgacc ttcgtctgtg tactatttgt tggcgtgatt ctgagcaacg
    17761 gtctgtcaat gatgggcttt taccgcgtct ttgagcgagc ggtatccgtg ctgggtaacg
    17821 taagcctgtc gttgttcctg gcgatggcgc tgatggggct gaaactgtgg gagctggctt
    17881 cgctggcgtt gccgatgctg gcgattctgg tggtacagac catcttcatg gcgttgtatg
    17941 ccatcttcgt tacctggcgc atgatgggca aaaactacga tgcggcagtg ctggctgcgg
    18001 gtcactgtgg ctttggcctt ggtgcgacac caacggcaat cgctaacatg caggcgatca
    18061 ctgaacgctt tggcccttcg cacatggcgt tcctggtcgt gccgatggtc ggtgcgttct
    18121 ttatcgatat cgtcaatgcg ctggtgatta agctgtattt gatgttgccg atttttgccg
    18181 gttaaccgat gaagcggcgg tcttctcacc gccgcttttt acgcattcga gtaacgttct
    18241 gtttctggca tccagcgttc tatcagggct tttgcctgtt gtgggtaacg ttcgtgaata
    18301 tgtcgcgcca ggcgctgaac ttccgggatc atcgcctgat cgcgcagtaa atccgccact
    18361 ttaaattcag cattccccgt ctgacgtgta cctaacaatt cgccagggcc gcgaatctcc
    18421 agatctttct gtgcaatcac aaaaccatcg ttgctgtcgc gtagtacctg caggcggatt
    18481 tgcgcggttt tggagagcgg cgttttgtag agcagcacgc agtgagaggc caccgcgcca
    18541 cgacccacgc ggccacgtaa ctggtgtaac tgcgccagac ccagacgctc cgggttttcg
    18601 ataatcatca gactggcatt gggtacatca acaccgactt caatgacagt agtggcaacc
    18661 agcaggtgta gctcgccttg tttaaacgat gtcatcaccg cctgtttctc ggcgggtttc
    18721 atccgtccgt gtaccaggcc aacattcaac tctggtagcg ccagtttcaa ctcttcccag
    18781 gtcgcttccg ccgcctgcgc ttccagtaat tccgactctt caatcaacgt acaaacccag
    18841 tacgcctggc gcccttcagt catacaggcg tggcgcacgc ggtcaatgat atcgatacgg
    18901 cgggtatcag gaatagcgac cgtagtcact ggcgtgcggc ctggtggcag ctcatctatc
    18961 accgaggtat cgagatcggc atacgcagtc atcgccagcg tgcgggggat gggcgtggcg
    19021 gtcatgatca actgatgcgg atggaagccc tgctgctggc ctttctccca taacgccaga
    19081 cgctgatgta cgccaaaacg atgctgttcg tcgataatca ccagcgccag gccgttaaac
    19141 tgcacctgtt cctggaaaat ggcgtgcgta ccaacaatca tctgcacctg accgctggcg
    19201 atggcttctt gctgagccag tcgtgcttta cctttctgtt ttccggcgag ccagcctact
    19261 tcgataccga gcggtgcaaa ccagttacgg aagttattgg cgtgctgctc ggcgagtaat
    19321 tcggttggtg ccatcagtgc cacctgtttg ccgtgggcaa ccgcacgcaa cgcggcgagg
    19381 gcggcgacca gcgttttacc ggaacctaca tcgccctgca ccagacgcat catcggcaca
    19441 tccagcgcca tatcgcgctc gatctccgcc actacgcgtg cctgtgcgcc cgttggcttg
    19501 aacggtaagg cggcgaggag tttatttttc agcgcgtcat tggcgctcag cggctgagca
    19561 tgaaaacgtt gtgcaccggc acgtaacgcc aacatgctga ggttgtgcgc cagcaactct
    19621 tccagaatca gccggcgttg tgccggatgc tgcccggttt cgagatcgct aagctgtagc
    19681 gtcggcggcg ggcggtgcaa agtgcgcaac gcttccggta gcgtcatcat tccttgtgac
    19741 agttccggcg gcaggagttc ttcaatggcg catgtgtcga gcagatccag cgcctggtcg
    19801 gttaatttac gcagcgtagc ctgctttacg ccttccgtag ttggataaac cggtgtgagc
    19861 gtttcctgta actctggcgt gctgagatcg ccctgtacgc ggtattccgg gtggatcatc
    19921 tccgcaccat atttgccgcg ctttgcttcg ccataggcca gcacgcggcg gcctgtcgcc
    19981 aggctatttt tcattgccgc attgaagttg aaaaagcgca tagtgagaat gccggaaccg
    20041 tcgctgatct ggcaggtcat catccgccga ccgccgaagg agatattgca gttcagcact
    20101 tcgccttcca ccgtggcata aacacccggc agtagttcac cgatggggta gagatgtgtg
    20161 cgatcttcgt agcgcagagg aaggtgtaag agcaaatcct gtacggtatg caggttgatt
    20221 ttcgccagct tgttactaag cgctgcgcca acgcccgtta gggaactgag cgggacagca
    20281 tctaacaggc gacctttcat cgcgcttacc ctgcggcctg catggtggac caccagtcag
    20341 catcggcttc aatctcgcct tgctgattaa cgtgggggta gggcagacct ttgcgttttg
    20401 cgactttcgc cagcaccgga tagccgcctt caaacagcag gcgttgttgc tcggcttccg
    20461 gcaacatgct gttttcacgc aggtacatgc ccgcattttg ccgctggcgc tgggcttcgt
    20521 aaagaatgag ggctgaggca acggaaacat tcagcgactg caccatgccg atcatcggaa
    20581 tgatgatatc ctgatccgcc agggccaacg cttcctgcgt gatgcccgtt ttctcctgtc
    20641 ccatcaaaat acaggtcggg cgagtgtaat caatttcgcg gaaatcaaca gcgttatcag
    20701 aaagatgggt tgccagaatc tgcatgccct ggcctttcag atgagcgacg gcatcgccaa
    20761 tggtgcggtg cgttttcacc tgtacccagc tgttactacc cgcagctgcc gaagccatgg
    20821 tgcgcatccg gctgccaggc cagacagcat gaacttcatg tacgccaacg gcatctgcgg
    20881 tacgaataat cgcagaaacg ttatgaggtt tgtggacctg ctccatgcag acggtcagat
    20941 caggctgccg cctggcgagc atttcgcaga tgcgtgcata acgtgttggg ttcataaaac
    21001 attaatttcg gtttcgggtg actttaatca cgtctggcat cacgcggatt ttgcgcatga
    21061 tattcgccag atgcacacgg tcacgggcgg tcagacgaat aaaggcgcta tagacgcgac
    21121 catctttctc ttccgtattc aaactttgaa tattcgaggt cgtggtgtta attgccgccg
    21181 tcaggtttgc cagcgcgccc tgatgattga acatctccac cttgatttcg gtgatgaatt
    21241 cctgcgccgt ctctttatcc cattcaacag ccataaactt ctctggctct ttctggtagc
    21301 cacggatgtt acggcaggat tcatggtgga tcaccagacc tttaccgggg ctgacgtggg
    21361 cgataatcgg gtcgccagga attgggcggc agcatttcgc aaaggtgatc agcacgccat
    21421 cggcaccttt aatgggcaga tgtccgtgac tttgggttgc cggtggaatg gaggcgtccc
    21481 catgttgcag atttttcgcg accaccacgc tcattgcgtt accaaggccg atttctgcca
    21541 gcagatcgtc aagcgttgcc agcttcatgc ggtccagctc gcgctgaata ttttcctgcg
    21601 ggatttcatt gagcttacgg ctaccaccca acgcatggtt gagcagacga cggcccaggc
    21661 ttacagaatc atcacgcttg aggtttttca gcaactgacg aattttggcg cgcgctttcg
    21721 agctgacgac aaagttcagc cacgcggcat tcgggcgagc acccggtgcg gtaatgattt
    21781 caacggtttg accgctagta agcggctgcg acagcgggta aggctggcgg tcgacgcgtg
    21841 cgcccacgca ggcatggccg atatcggtat gcaccgcata agcgaagtcg acgggcgttg
    21901 caccggcagg cagttcgaca atgcgccctt ctggtgtgaa aacgtaaatc tcatccggga
    21961 agagatcgga tttaacgctc tcgataaatt caaacgagct accggcactc tgttgcagct
    22021 ccagcaggct ttgcatccag cgctgggcgc gtatttgcgc ggtagtgctg gtttcgccgt
    22081 gctctttata agcccagtgc gcggcaacac ccatctccgc catctgatcc atatcttcgg
    22141 tacggatctg gacctcaacc ggcacgccgt gcgggccaat catcgaggtg tgcaaagact
    22201 gatagccgtt cgcttttgga atggcgatat agtctttcac gcggcccgga cgcggcttgt
    22261 acaggctgtg catctggccc agcacgcgat aacaggtgtc agaatcattg acgatcacgc
    22321 ggaaagcgta gatgtccatg attgagtgaa aacgctgctc tttgagcacc attttgcagt
    22381 aaatcgaata aagatgcttt tcgcgaccac tgacgcggca cggtattccc gcttcctgca
    22441 aacgcccttc gatttcagaa agaatcttct ggatcatctc tttacggttg ccgcgcgcgg
    22501 ctttcaccac ttctttgatt acgcgataac ggttgggata cagcgcctca aaacccagct
    22561 cttcgagttc ggttttaatg tggtggatac ctaaacggtg cgccagcgga ctgtaaattt
    22621 cgagagtttc acgggcgatg cggcgacgtt tgtccgggcg aagtgagccc agcgtgcgca
    22681 tgttgtgggt acggtcggca agtttgatga gaatgacgcg gatatcctgc accatcgcca
    22741 taatcatctt gcgaaagttt tcggcctgcg cctctttctt atcgcggaac ttgagtttat
    22801 caagtttcga caccccctct accagctcgg cgacgctttt accaaaaagc tgctccatgt
    22861 cctggtaggt ggcgggtgta tcttcaatca catcatgcag cagcgccgcc atcagcgttt
    22921 catagtcgag tttcatctcg gccagaatgc aggcaaccgc caccgggtgc gtgatatagg
    22981 gttcaccgct tgaacgtgtt tgcccctcgt gagcatcacg tgcaacgaga tacgcctgcc
    23041 gcagacgctt gatttggtct tccggcaggt agttttgaat cagctgattc aggctttcaa
    23101 acagatacaa gggcgacccg ctttgtgatt aacgacgacc ttcagcaata gcggtaacgg
    23161 cttgtaattc agcggcttcc tgctcttgct gttcctggcg ttcgcgaacg tcgaggatct
    23221 ggttgttgat cagaccttct tcgatttcgc gcagcgcgat tacagtggtt ttatcgtttt
    23281 cttccggtac cagcggatcc tttccgccta cctgcatctg acgagcgcga cgcgcggcga
    23341 ccagtaccag gtcaaaacgg ttaccaattt tctctacagc gtcctgaaca gttacgcgtg
    23401 ccatacttaa aaagctccac aggtgaagaa atgactgggc atgatactga aatcaggttc
    23461 agtctgccaa caatttgctg attaaagcgt catgacgctg cttttggcgg ctcatgcgca
    23521 gacgttcggc gcgaataatg gtcttcaaat cggtcaacgc ggtatcgaag tcatcattca
    23581 caatcaggta atcatattcg gcgtaatggc tcatttctgc aacagcttgc gccatacgct
    23641 ttgcaatgac ctcttcgctg tcctgaccgc gaccgcgtag acggcggtcc agttcgattt
    23701 tagacggcgg taaaataaag atactccgcg cgtgcggcat cttctggcga atttgctgcg
    23761 caccctgcca gtcgatatcg agaaaaacat cgacaccggt cgccagtact tgctcaatgg
    23821 cctcacgcga agtgccatag taattaccaa aaacttctgc gtgttcgagg aacgcatctc
    23881 tactaatcat ttctttaaat tcatcatgat taacaaagaa ataatgttca ccgtggactt
    23941 caccaggacg cggttggcgt gtggtgtgtg aaacagaaac ctgggtgtca tacaacggtt
    24001 gggtttttaa taaagcctga atcaggctgg atttacccgc gccactgggg gcagaaacaa
    24061 tataaagcgt gccttgagcc atgagtatct ttcgtatgtg attagcgaaa taaagcctac
    24121 atacgagctt attatacacg gcgcggccat gtgacgtagc ccttgctcac actttttgcg
    24181 caaagttgtt gcattttgcg tggctgcttc cgcatttgcc tgtgattcac tgcaataaag
    24241 acgattctca ggaagcgtcc tcgcagactc cgtttttgcc actcggcttt gctttcacca
    24301 cgttgtatat ctgcctgact atctacagcg gaggtgatga tgaaagtatg gatggcgata
    24361 ttaataagta tcttgtgctg gcaatcatcg gcgtgggcgg tctgtccggc ctggtcgcca
    24421 gccagagcac aggaagaaat ttcccgcctg caacagcaaa taaaacagtg ggacgatgac
    24481 tactggaagg aagggaaaag tgaggtggaa gacggtgttt acgatcagtt aagcgcccgt
    24541 ctcacgcagt ggcaacgctg ttttgggaac gagactcgcg atgtgatgat gccgccttta
    24601 aacggtgcgg ttatgcatcc ggttgcccat acgggtgtac gcaaaatggc ggataaaaac
    24661 gcattaagtc tgtggatgcg cgaacgtagc gatctttggg tgcagccaaa agttgatggc
    24721 gttgcggtaa ccctggttta tcgggacggg aaactgaaca aagcaatcag tcgcggtaat
    24781 ggcctgaaag gcgaggactg gacgcagaaa gttcgcttaa tttccgctgt gccgcaaacc
    24841 gttagcgggc ctttagccaa cagcacgctt cagggggaaa tatttctcaa gcgtaagggg
    24901 catatccaac aacaaatggg gggaataaat gcccgcgcaa aagttgctgg cctgatgatg
    24961 cgccagggga atagcgatac gctaaactca ctggctgttt ttgtctgggc atggccggat
    25021 ggaccgcatt taatgaccga tcgtttaaag gatctggcta ccgcaggttt tactttaacg
    25081 cagacgtata cccgcgcggt taaaaatgct gatgaggttg cgcacgtacg caatgagtgg
    25141 tggaaagcaa aattaccctt cgtcaccgat ggcgtggttg tgcgagcggc gaaagaaccc
    25201 gaatcccgtc attggttacc gggccaggcg gagtggctgg tggcctggaa atatcaacct
    25261 gtagctcagg ttgccgaagt gaaggcgatt cagtttgcgg tgggtaagag cggtaaaata
    25321 tcggtggtgg cgtcactcgc acctgtcatg ctggatgata aaaaagtcca gcgggtgaat
    25381 attggttccg tcaggcgctg gcaggagtgg gatattgcgc ctggtgatca gatcctcgtc
    25441 agccttgccg gtcaggggat tcctcgcatt gatgatgtgg tctggcgcgg agcagaacgt
    25501 acaaaaccga caccaccaga aaaccgcttt aactcgttga cctgctactt tgcttctgat
    25561 gtttgtcagg aacagttcat ttcacgctta gtctggctgg ggtcaaaaca ggttctgggg
    25621 cttgatggca ttggtgaggc cggctggcgc gccctgcatc agactcatcg ctttgagcat
    25681 atcttttcct ggcttttatt aacgccagag caattacaga acacgccggg gatcgcgaaa
    25741 agtaaaagtg cgcagttatg gcatcagttt aatctggctc gtcagcagcc ttttactcgc
    25801 tgggtgatgg caatgggaat accgctaacc cgggcggcgc ttaatgccag tgatgaacgg
    25861 tcctggtcgc aacttttatt tagcacggaa cagttctggc agcagctgcc ggggactgga
    25921 tcggggcgcg ccagacaggt tattgaatgg aaggaaaatg cgcaaatcaa gaagctgggc
    25981 agttggctgg ctgcccagca gatcacaggt tttgaacctt agtgaccttc gtggctgtag
    26041 taaaagaccg gtaatcccag cttaagacgt agcgccagta aacgggcaaa aaagccgaat
    26101 accagggtgg agataatcac cacatcatgg ttagaaacat agtgttgcag cgcgatgtac
    26161 agcacggcgg aggcaaatga gacaccggca tacaattctt tctggaatac cagcgggatg
    26221 cgtttacaga acatatcgcg caaaacgccg ccaaatacgc cggtcgtcac agctgcgaca
    26281 acggcaatga tcgggccgtg tcccatatcc agcgcaacct gtgcgccaat gatagaaaag
    26341 actaccagtc cgagcgcatc gagcaccaga aacactttgc gcaggtaagg cattacagga
    26401 gcaacaatag tggtgagtac tgcggcggta gcgacgataa tcacatattc cgggtgtttg
    26461 acccagccga gcggatagtg gcccagcaga atatcgcgca ctgaccctcc gccaattgcg
    26521 gtggcggtcg caataataat tacgccaaat gtatccatgc gccgtcgtcc ggccgccagc
    26581 gcccccgtca tggcttcggc agtaatgcca accaaataca aaatgtgtaa cagcatggtt
    26641 ctctccctaa aaatctgtct ggaagggtag cgatttgtgc gcattgtcac gattgagatt
    26701 ttttaaggag agaatagatt gataatttgt agttatgtga aattgctatt ttctgtagta
    26761 agaatgcatt tttctgattt tgtgataagt aaaatgcatt agaaaaagta tggagtaaat
    26821 aaatatgagg acgaaactat atgcgtagcg ccgctgtaat taacaacggc ctacggagat
    26881 atgtcgctta ctcgatattc tgaatctgct cgcgcatctg ctcaatcaac actttcagct
    26941 cgatggcgga gtttgtcact tcggcgttga tagatttcga cgcaagagtg ttcgactcgc
    27001 ggttgaactc ctgcatcata aagtccagac gacgaccaac ggcttctttt ttcttcagaa
    27061 tgttgtaggt ctctttgaca tgcgcttcga ggcggtccag ttcttcggca acgtcaattc
    27121 gttgtgccag cagaaccaac tcctgctcca gacggttgtt ttccagttgc acctgagcat
    27181 cttccagctt cgctaccaga cgctcacgct gccattgcag gatttccggc atatgggcgc
    27241 ggactttgac cacttcggcg gtgactcctt ccagacgctg ctcaatcaac actttcagcg
    27301 cctgaccttc ggtttcgcgc gcgatgataa agtcatccag ggtgccatcc aacgccgcga
    27361 gaatttcagc tgcaatcgcg tcgagatctt gctcctgggc tgccatcacg cccggccagc
    27421 gtagaatatc aaccgggttg atttctcctt cgtcgctctg catttttacc cagttcgcgg
    27481 cagttaccag ctgtttagcc agtttttcgt tgaggatcag ctcaccttgc gcgctaacat
    27541 ctggctcata gcgcagggta cattccactt taccgcgcgt caggcgagaa cgaatacgct
    27601 cgcgaacgac aggttcaagg ctacggaact gctccggcag acgaaagtaa gtttccagat
    27661 aacgctggtt taccgagcgc atttcccagg ttgcgctccc ccattcaccc ttgatttcac
    27721 gccgggcgta ggcggtcata ctgcggatca tagacgttcc tgtttataaa aggagaggtg
    27781 gaaggattat agccatcgat gccttgtcag gataggaata accgccggaa gtccgtataa
    27841 tgcgcagcca catttgtttc aagccggaga tttcaatatg cgtccagcag gccgtagcaa
    27901 taatcaggtg cgtcccgtta ccctgactcg taactataca aaacatgcag aaggctcggt
    27961 gctggtcgaa tttggcgata ccaaagtgtt gtgtaccgcc tctattgaag aaggcgtgcc
    28021 gcgcttcctg aaaggtcagg gccagggttg gatcaccgca gaatacggta tgctgccgcg
    28081 ttctactcat acccgtaatg cccgtgaagc ggcgaagggt aagcagggcg gccgcacgat
    28141 ggaaatccag cgtctgatcg ctcgtgcatt gcgcgccgcg gtagatttga aagccctcgg
    28201 tgagtttacc attacgctgg attgtgacgt acttcaggcc gatggcggca cccgcactgc
    28261 ttctatcacc ggtgcttgtg ttgcactagc cgatgccctg aataaactgg tggaaaacgg
    28321 caagctgaaa actaatccaa tgaaagggat ggtagccgcg gtctctgtcg ggattgtgaa
    28381 cggcgaagcg gtttgcgatc tggaatacgt tgaagactct gccgcagaga ccgacatgaa
    28441 cgtagtgatg accgaagacg ggcgtatcat tgaagtgcag gggacggcag aaggcgaacc
    28501 gttcacccac gaagagttac tcaccttgtt ggcgctggcc cgagggggaa tcgaatccat
    28561 tgtagcgacg cagaaggcgg cgctggcaaa ctgattttta aggcgactga tgagtcgcct
    28621 tttttttgtc tgtagaaaag taagatgagg agcgaaggca tgaaaccata tcagcgccag
    28681 tttattgaat ttgcgcttag caagcaggtg ttaaagtttg gcgagttcac gctgaaatcc
    28741 gggcgcaaaa gcccctattt cttcaacgcc gggctgttta ataccgggcg cgatctggca
    28801 ctgttaggcc gtttttacgc tgaagcattg gtggattccg gcattgagtt cgatctgctg
    28861 tttggccctg cttacaaagg gatcccgatt gccaccacta ccgccgtggc gctggcggag
    28921 catcatgacc ttgacctgcc gtactgcttt aaccgcaaag aggcaaaaga ccacggtgaa
    28981 ggcggtaatc tggttggtag cgcgttacaa ggacgcgtaa tgctggtaga tgatgtgatc
    29041 accgccggaa cggcgattcg cgagtcgatg gaaattattc aggccaatgg cgcgacgctt
    29101 gcaggcgtgt tgatttcgct tgatcgtcag gaacgcgggc gcggcgaaat ttcggcgatt
    29161 caggaagttg agcgtgatta caactgcaag gtgatctcta tcatcaccct gaaagacctg
    29221 attgcttacc tggaagagaa gccagaaatg gcggaacatc tggcggcggt taaggcctat
    29281 cgcgaagagt ttggcgttta acgaaactcg ccggatgaaa agtcatccgg cgtcatatta
    29341 ctgcaactgt gcggcaatta gcggccagcg ggcgtcaaaa tcatccgttg ggcggtattt
    29401 aaactcgctg cggacaaagc gtgatagcat accttcacag aaggccagca actggcttgc
    29461 cagcagggtt tcatcggtgg tgtaaccttc accctcacgc attctctttt cacgcaatac
    29521 ctgacgcaac tgcgcttcaa tacgctcgaa cagctggttg atgcgccctt gcagacgatc
    29581 ctgttcgaac attagcgcat gaccagtgag gatgcgagtc aggccaggat tacgctcacc
    29641 aaaaccgagg agcaacaaca cgatcagacg caggcgcgct gtggtgtctt tctcatcttt
    29701 cagaatcaga ttgatgcgag taatcaggct atcttcgata aactcaatca ggctatcgaa
    29761 catgcgggtc ttactgggga agtggcgata cagtgccgct tcggaaacgc cgacagaggc
    29821 ggccagtttt gccgtcgtga tacgttggct tccatcgctg gattccagca tcagcgccag
    29881 agactgaagt atttcctcgc gacggttcct tttcgcagtt tgtttttctg ccatgttaca
    29941 aaatacccct gaaaataagc acttgccagg cggcacccac gctatgaccg caaacgaaat
    30001 gtttgcggct atgttatgac gttattcgga tgcgtatgtg ttactgacga ccagagtgac
    30061 caaagccgcc ttcaccgcgg tcggtggcgt cgaaatcttc caccagatta aattcagcct
    30121 gtactaccgg aacaaaaatc atctgggcga tgcgttcgcc aggttgaatg gtgaagctgt
    30181 cctgaccacg gttccacacg gaaatcatca actggccctg atagtcagaa tcgatcaatc
    30241 ctaccaggtt accaagcacg ataccgtgct tatgtcccaa tccggagcgc ggcagcatca
    30301 ttgccgccag tgaaggatcg gcaatatgaa tcgccagccc ggtcggaacc agcgtagtgt
    30361 cacccggagc cagttctacg gcgtcgtcga gacaggcacg caggtcaagt ccggcagagc
    30421 cagaggtggc ataagtcggg agcggaaatt ccttcccaac gcgcgggtcc agaatcttaa
    30481 cgtcgatttt tttcatcata acgggtcacg atctcgtcga gtaataattg gccaaggagc
    30541 tctttgcgct caagcggtaa gactttatct ccgtcctgcc agaaaaggtg taatgcgttg
    30601 ttgtcgctgt taaatccttg agttggctgg gaaacatcgt tcgcgcagat cagatcaagg
    30661 tttttacgga tacgtttttg ccgggcgtat tcttccacat tatttgtttc ggcggcaaat
    30721 ccaacgacgt agggtcgatg gtcttttagt gcggcaacgc ctgcgacgat atcggggttt
    30781 ttaaccattt ttattgttaa ttcatcaccc tgcgtggcct gctttttgat tttttctgag
    30841 gccacggtag ctgcgcgata atccgccacg gcggcgcaac cgataaaaat attttgctgc
    30901 tgtacagaag cattcacggc ggcttccatt tccagcgcgg tcatcacatc aacacgttta
    30961 acaaacggtg gcgtcggtag cgaaaccggg cctgatacca gcgtgacgtt cgcgccacga
    31021 cgggcagcag cggcggcgat agcaaaaccc atcttgccgg agctgtgatt agagatataa
    31081 cgcaccggat cgagcggttc acgcgtcggg ccggcggtaa tcataatgtt cagatgtttc
    31141 aggtcgttga cgggcgaaaa atgcgctacc gccatatcaa caatggttaa cggatcgagc
    31201 attcgccctg ggccgatatc accacaagcc tgactgccac tgtctggccc ccagatgagc
    31261 aaaccacggg aggcaagcac ctctaaatta tgctgcgtgg cagcggcacg gtacatctgc
    31321 tggttcatgg cggggagcac ggctacaggc gcaggtgtag ccagacaaat cgtcgatacc
    31381 aggtcattcg ccattccggc agcaacacgg gcaatcaaat ctgccgtggc aggggcgaga
    31441 atcactaaat cagcccattt acccagctca atatggccca tagcggcttc ggctgccggg
    31501 tcaagcagac tgtcggaaac gggataacca gaaaccgcct gcaagctaag tggggtgata
    31561 aaggcttttg ccgcttcggt catggctatg cggacgtcgg ccccgcgatc gcgcaaacga
    31621 cgcaccagtt caggggtttt ataggcagca ataccgccgc tgacgccgag aacgattttt
    31681 ttaccggcca ggctcatgat gatttttcct gttgggtgac accagaagtt ggcgatttta
    31741 tcacaatact tttgttgtcg tgccttcacc tgagaatcac tttgcgaggc gctttccagg
    31801 attgaaaact ggccgtcgat ttaacggaac ggctatgaca ggatgcgagc accacaaagg
    31861 aggtgaaggt gaaaaacaat gcacagctgt tgatgccgcg cgaaaaaatg ctgaagtttg
    31921 gtattagcgc cttaacggat gtcgagctgc tggcgttatt tctgcgtacc ggaacgcgcg
    31981 gtaaagatgt attaaccctg gcaaaagaga tgctggagaa tttcggctct ctttatggct
    32041 tgttaacctc tgaatatgaa caatttagtg gtgttcatgg aattggcgtg gcgaaatttg
    32101 cccagttaaa ggggattgct gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag
    32161 aaagcccttt actcagcccg gagatgacgc gggaattttt acaaagccag ctcacgggtg
    32221 aggagcggga gatctttatg gtgatctttc tcgactcaca acaccgggtt ataacgcata
    32281 gccgtctttt ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa attatccgcg
    32341 aagcgataaa aataaacgcc tcggcgctga tccttgcaca taatcaccct tcgggttgtg
    32401 ctgaacccag taaagcggat aaactcatta ctgaacggat aataaagagt tgtcagttca
    32461 tggatttacg cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt tcttttgccg
    32521 aacgcggctg gatttaaccc gctatgcgcg atccttcggg atctttgtct gttcgggact
    32581 tgagcacatc gctgagtcag cgtatactac gccacctttg agaatctcgg gtttggcatt
    32641 tgggcctggc aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa agcctgacga
    32701 ggcgccaata ccccatacga agctcgagct aatttgattt ttggagaata gacatgtccc
    32761 gagtctgcca agttactggc aagcgtccgg tgaccggtaa caaccgttcc cacgcactga
    32821 acgcgactaa acgccgtttc ctgccgaacc tgcactctca ccgtttctgg gttgagagcg
    32881 agaagcgttt tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag
    32941 gcatcgatac agttctggct gaactgcgtg cccgtggcga aaagtactaa gtacttagag
    33001 gaaataaatc atggctaaag gtattcgtga gaaaatcaag ctggtttctt ctgctggtac
    33061 tggtcacttc tataccacta cgaagaacaa acgtactaag ccggaaaaac tggaactgaa
    33121 aaaattcgat ccagttgttc gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt
    33181 ctcgctttga tgtaacgaaa aacctcgctt cggcggggtt ttttgttatc tgcttgcccc
    33241 catattgatt gcatctgttc attcctggag atgctatgcc tgaattaccc gaagttgaaa
    33301 ccagccgtcg cggcatagaa ccgcatctcg ttggtgcaac cattcttcat gcggtggtgc
    33361 gcaacggacg cttgcgctgg ccggtttcag aagagatcta ccgtttaagc gaccaaccag
    33421 tgcttagcgt gcagcgccgg gctaaatatc tgctgctgga gctgcctgag ggctggatta
    33481 tcattcattt ggggatgtct ggcagcctgc gcatccttcc agaagaactt ccccctgaaa
    33541 agcatgacca tgtggatttg gtgatgagca acggcaaagt gctgcgctac accgatccgc
    33601 gccgctttgg tgcctggcta tggaccaaag agctggaagg gcacaacgtg ctggcccatc
    33661 ttggaccgga gccgcttagt gacgatttca acggtgagta cctgcatcag aagtgcgcga
    33721 agaaaaaaac ggcgattaaa ccgtggctga tggataacaa gctggtggta ggcgtaggga
    33781 atatctatgc cagcgaatcg ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat
    33841 cactgtcgct ggcggagtgt gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt
    33901 cgattgagca gggcggtaca acgctgaaag attttctgca aagtgatggt aaaccgggct
    33961 atttcgctca ggaattgcag gtttacgggc gaaaaggtga gccgtgtcgg gtgtgcggta
    34021 cgccgattgt ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga
    34081 agtaattgat gcgcgccgga tggcatacca tccggcataa actctacgct aacttcgcca
    34141 tcagcgcctg atggacattc tccggcagga aatgggtgac atcgccctga tggcgcgcaa
    34201 cctctttcac caacgatgaa gagataaacg accactcttt cgacggcatc agaaacacac
    34261 tttccagttc tggcattaag tggcgattca tatgcgccag ctgcatttca tattcaaaat
    34321 ccgccaccgc acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg
    34381 ccatcaaatc actaaacccg accacttcca cgttctccag atgcgcggtt gcctgctgtg
    34441 ccagctccac acgctcttcc agggtaaaca tcggtttttt actgggacta gcggcaatcg
    34501 ccagaataac gtgatcgaac atttgcgagg cgcgcgtcac gatatcgata tgaccattgg
    34561 taatgggatc gaaagtaccc ggataaatcg cccgtttttg cataacaacc tcaatgcgtt
    34621 ttcggtggca ggtaaggttc cagcagttga agcagacgct gtagtgcgcc ctggttttga
    34681 tacagcactt caacggcatg acggccatag aaactacggt aatcggcgtc ggtgagtaaa
    34741 gaggaaacct cttttgcaag cgtagtggca tcggtaacgg taatcagccc gcttgcctgc
    34801 tccagccgcg cgcaaatgtc tttaaagtta aacgtatgcg gccccatcaa taccggaata
    34861 gcgtgtgcgg ccgcttccag cggattatgc ccaccacgtt caaccagtga accgccaaca
    34921 aaggcgagat cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc gccaaccaca
    34981 acctgcgtgc tggtggaggg gacttcccct gaagatcgtg tgatatagct tagtccagcc
    35041 tggcggacaa ggttaatcgc atccgggaag cgttctggat gacggggtac caggatgagc
    35101 aataaattcg ggaattgctg taacaatgcc tgatgtgcgg cgatcaccac actctcttcg
    35161 ccttcatgag tgctggtggc aatccatacc gggcggtgtg gtgcccattg gcggcgcagc
    35221 gtcacggctt tagcagccaa ctgcggcgtt acagaaatat cgaatttcag gctaccggta
    35281 acggtcacct gattattttt tgcgcccaac gccacaaaac gagcaccatc ttcttcattt
    35341 tgggcggcaa tcagcgtaat acgacgcagc aagcgacgga cgaatttacc cagtttggca
    35401 taacctgcgg ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg aattttacgt
    35461 ttatgtagcg ccgcaatcag gttaggccat agttcggttt ccataatcaa caccagttta
    35521 gggtcgactt tattcaggaa acggttgagt gcatcgggca ggtcatacgg cagataaacg
    35581 tgctgaacat ccttcccgaa agccgattgt acgcgctccg aaccggttgg agtcatggtt
    35641 gttacggtaa tcggtaaatc aggataacga tgacgcagtg cgcgcaccaa cgggatcgcc
    35701 gccagagttt caccgacgga gacggagtgc agcataatgc cgcctggttt tagcggatgg
    35761 cggtaaaaac cgtaacgttc accccagcgt tttcgatagg ccggagcctt acgtccgcgc
    35821 acccagagcc gtatccagat cagcggctga ataaggtaga gaagggcggt gtaaagcaat
    35881 tcgagcatag taaatagctg acttatggat gtgctgggga ttctatgtat ttagctgtgg
    35941 ctttaccatt acttttccca tttttgactt aaatagcaat ttacccgcca attcttgctg
    36001 aacatctata ctttttttgt atttttatgc gattcattga tttctgcagt acgtgcagga
    36061 aggaatcgca gagcaacagt aaaagaatat ggttgctaat gattttattt gattgatttc
    36121 tttattgaaa atcaacaatg tgcttaaaaa tcattcgagt cggattgccg cgaaagaagt
    36181 gatacactag cgcgtcaata tcagtgcggt atcagtattc aggtagctgt tgagcctggg
    36241 gcggtagcgt gctttttctg cttcacctta atcggataat ctcaacaaaa agagttactt
    36301 gtggataagc catttcgaag aattttgctc attaagatgc gttttcatgg ggatatgtta
    36361 ttaactactc ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg
    36421 ctgctttatc aggacaccat cccgatcctg tctgaaaatc cagagattaa cgcgctctac
    36481 ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt tcatctcatc
    36541 aaggtattac gtgccaataa gtatgacctt atcgttaatc ttaccgatca atggatggtt
    36601 gctatactgg ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg
    36661 cagtctgctt tttggcgtaa tagtttcact catttggtgc cgttgcaggg tggaaatgtg
    36721 gtggaaagta acttatccgt gctgacgcca ttgggacttg aatcgttggt gaagcagaca
    36781 accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt
    36841 gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa atgctgggac
    36901 aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga agtcgttctg
    36961 acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca gggatgccag
    37021 acgccaccag taacggcgct ggccggaaag gtgaccttcc cggaacttgg tgcgttaatc
    37081 gatcatgcgc agctgtttat tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt
    37141 aatacgccgc tgatatcgct gtttggtgcg acagaccata ttttctggcg tccctggtca
    37201 aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt
    37261 gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc agctgtcgat
    37321 aaattacttc cctcctccac gacaggtacg tcgttatgat cgttgctttt tgtttatata
    37381 aatattttcc ctttggcggt ttgcagcgcg attttatgcg tattgctcag acagtcgctg
    37441 cccgaggtca tcatgttcgg gtttataccc agtcgtggga aggcgaatgc cctgatgtat
    37501 ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat gcggaatatt
    37561 ttgcctgggt gcaaaaacat ttacgtgaac accccgtcga tagagtcgtt ggatttaata
    37621 aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag aaagtagcgc
    37681 aggagaaagg ctttttctat cgcctgacgt cacgttatcg ccattatgct gcctttgagc
    37741 gggcaacctt cgaacagggc aagccgacac agctgctgat gctgacagat aagcaaatcg
    37801 ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg cctccgggga
    37861 tttatcctga tcgtaaatat agccagcagc cagccaatag ccgtgaaatc ttccgtaaga
    37921 agaatggaat aaccgaacaa caatatttat tgttgcaggt cggttcagac ttcacgcgta
    37981 aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg cgccacaaca
    38041 cattactata tgttgttggg caggataaac cgcgaaaatt tgaggcgctg gcagaaaaac
    38101 gcggcgtgcg cagtaatgtt cacttcttct cggggcgcaa cgatgtctcg gaattaatgg
    38161 cggcggcgga tttattactt catcctgcct accaggaagc ggcgggaatt gtgctgctgg
    38221 aagcgataac tgcaggatta ccggtactaa caacagccgt ttgtggctat gcgcattata
    38281 ttgtcgacgc caattgcggc gaggctattg ctgagccatt ccgccaggaa acattgaatg
    38341 agattttacg caaagcgtta acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc
    38401 gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg gacatcataa
    38461 cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg cggtaaagat
    38521 ccttttgggg aagttaaaac cttgcagggt gaggtatttc gtgaactgga aactcgccgc
    38581 actctgcgct ttgaaatggc gggcaaaagc tattttctca aatggcatcg cggcacgacc
    38641 ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg cgcagaccgc
    38701 gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta tggggtggca
    38761 ttcggcgaaa aaggcattaa tccgctgacc agaacctcgt ttattattac cgaagatctg
    38821 acaccaacca tcagtctgga agattattgt gctgactggg cgaccaaccc tccagatgtt
    38881 cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgcg
    38941 ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc tttttccggt
    39001 aaggaagagg tgttaaaaat ttcagtaatt gacctgcacc gggcgcagat acgtgcaaaa
    39061 gtaccgcgtc gttggcggga taaagatctt attggacttt atttttcttc gatgaatatt
    39121 ggcctgactc agcgggatat ctggcggttt atgaaagtgt attttgtcgc cccgcttaaa
    39181 gacattatca agcaggaaca agggctgctg tcgcaagcag aagaaaaagc cacaaaaatc
    39241 agggaaagaa cgattcgaaa atcgttgtaa cccgtaactt atttttgcca aaattttgga
    39301 tacagaataa atatgtctca actcaatgat agtgacatca
//
LOCUS       DADTFG010000024        39081 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.024, whole genome shotgun sequence.
ACCESSION   DADTFG010000024 DADTFG010000000
VERSION     DADTFG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.024"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            170..1741
                     /gene="garD"
                     /locus_tag="J1138_001215"
     CDS             170..1741
                     /gene="garD"
                     /locus_tag="J1138_001215"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBA8453042.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAECHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1793..2617)
                     /gene="agaR"
                     /locus_tag="J1138_001216"
     CDS             complement(1793..2617)
                     /gene="agaR"
                     /locus_tag="J1138_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBA8453043.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            2866..4146
                     /gene="kbaZ"
                     /locus_tag="J1138_001217"
     CDS             2866..4146
                     /gene="kbaZ"
                     /locus_tag="J1138_001217"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBA8453044.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARVTLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKAAALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4169..4642
                     /gene="agaV"
                     /locus_tag="J1138_001218"
     CDS             4169..4642
                     /gene="agaV"
                     /locus_tag="J1138_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBA8453045.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDLVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4653..5432
                     /locus_tag="J1138_001219"
     CDS             4653..5432
                     /locus_tag="J1138_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBA8453046.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5422..6300
                     /locus_tag="J1138_001220"
     CDS             5422..6300
                     /locus_tag="J1138_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBA8453047.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            6318..6752
                     /locus_tag="J1138_001221"
     CDS             6318..6752
                     /locus_tag="J1138_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA8453048.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6749..7882
                     /gene="nagA"
                     /locus_tag="J1138_001222"
     CDS             6749..7882
                     /gene="nagA"
                     /locus_tag="J1138_001222"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBA8453049.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDV
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMAGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            8232..9386
                     /locus_tag="J1138_001223"
     CDS             8232..9386
                     /locus_tag="J1138_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HBA8453050.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVKLANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPEMINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            9399..10259
                     /gene="kbaY"
                     /locus_tag="J1138_001224"
     CDS             9399..10259
                     /gene="kbaY"
                     /locus_tag="J1138_001224"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HBA8453051.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVELVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            10426..10902
                     /gene="agaB"
                     /locus_tag="J1138_001225"
     CDS             10426..10902
                     /gene="agaB"
                     /locus_tag="J1138_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBA8453052.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            10941..11744
                     /gene="agaC"
                     /locus_tag="J1138_001226"
     CDS             10941..11744
                     /gene="agaC"
                     /locus_tag="J1138_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBA8453053.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            11734..12525
                     /gene="agaD"
                     /locus_tag="J1138_001227"
     CDS             11734..12525
                     /gene="agaD"
                     /locus_tag="J1138_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBA8453054.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQADGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            12526..13293
                     /locus_tag="J1138_001228"
     CDS             12526..13293
                     /locus_tag="J1138_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBA8453055.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSHLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVANLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(13343..14206)
                     /gene="rsmI"
                     /locus_tag="J1138_001229"
     CDS             complement(13343..14206)
                     /gene="rsmI"
                     /locus_tag="J1138_001229"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBA8453056.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQENDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            14271..16307
                     /locus_tag="J1138_001230"
     CDS             14271..16307
                     /locus_tag="J1138_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HBA8453057.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRETT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            16265..16660
                     /locus_tag="J1138_001231"
     CDS             16265..16660
                     /locus_tag="J1138_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBA8453058.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            16680..17270
                     /gene="diaA"
                     /locus_tag="J1138_001232"
     CDS             16680..17270
                     /gene="diaA"
                     /locus_tag="J1138_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBA8453059.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            17280..17855
                     /gene="yraP"
                     /locus_tag="J1138_001233"
     CDS             17280..17855
                     /gene="yraP"
                     /locus_tag="J1138_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBA8453060.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(17968..19008)
                     /locus_tag="J1138_001234"
     CDS             complement(17968..19008)
                     /locus_tag="J1138_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA8453061.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALNYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWDFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPTKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(19081..19716)
                     /locus_tag="J1138_001235"
     CDS             complement(19081..19716)
                     /locus_tag="J1138_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBA8453062.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            19844..20362
                     /gene="yhbO"
                     /locus_tag="J1138_001236"
     CDS             19844..20362
                     /gene="yhbO"
                     /locus_tag="J1138_001236"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBA8453063.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVILGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(20342..20785)
                     /locus_tag="J1138_001237"
     CDS             complement(20342..20785)
                     /locus_tag="J1138_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBA8453064.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            20836..21138
                     /gene="yhbQ"
                     /locus_tag="J1138_001238"
     CDS             20836..21138
                     /gene="yhbQ"
                     /locus_tag="J1138_001238"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBA8453065.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAVFAELLSSLQTPEIKSD"
     gene            complement(21125..21628)
                     /locus_tag="J1138_001239"
     CDS             complement(21125..21628)
                     /locus_tag="J1138_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA8453066.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(21622..22146)
                     /locus_tag="J1138_001240"
     CDS             complement(21622..22146)
                     /locus_tag="J1138_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBA8453067.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            22355..23350
                     /locus_tag="J1138_001241"
     CDS             22355..23350
                     /locus_tag="J1138_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBA8453068.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVNGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            23359..24237
                     /locus_tag="J1138_001242"
     CDS             23359..24237
                     /locus_tag="J1138_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBA8453069.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            24318..25325
                     /locus_tag="J1138_001243"
     CDS             24318..25325
                     /locus_tag="J1138_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBA8453070.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(25443..26687)
                     /gene="mtr"
                     /locus_tag="J1138_001244"
     CDS             complement(25443..26687)
                     /gene="mtr"
                     /locus_tag="J1138_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBA8453071.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(26841..28730)
                     /locus_tag="J1138_001245"
     CDS             complement(26841..28730)
                     /locus_tag="J1138_001245"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HBA8453072.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(28723..28803)
                     /gene="yrbN"
                     /locus_tag="J1138_001246"
     CDS             complement(28723..28803)
                     /gene="yrbN"
                     /locus_tag="J1138_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBA8453073.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(28910..29794)
                     /gene="nlpI"
                     /locus_tag="J1138_001247"
     CDS             complement(28910..29794)
                     /gene="nlpI"
                     /locus_tag="J1138_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBA8453074.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(29903..32038)
                     /gene="pnp"
                     /locus_tag="J1138_001248"
     CDS             complement(29903..32038)
                     /gene="pnp"
                     /locus_tag="J1138_001248"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBA8453075.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLDEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(32285..32554)
                     /gene="rpsO"
                     /locus_tag="J1138_001249"
     CDS             complement(32285..32554)
                     /gene="rpsO"
                     /locus_tag="J1138_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBA8453076.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(32703..33647)
                     /gene="truB"
                     /locus_tag="J1138_001250"
     CDS             complement(32703..33647)
                     /gene="truB"
                     /locus_tag="J1138_001250"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBA8453077.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAKLLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(33647..34048)
                     /gene="rbfA"
                     /locus_tag="J1138_001251"
     CDS             complement(33647..34048)
                     /gene="rbfA"
                     /locus_tag="J1138_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBA8453078.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(34212..36884)
                     /gene="infB"
                     /locus_tag="J1138_001252"
     CDS             complement(34212..36884)
                     /gene="infB"
                     /locus_tag="J1138_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBA8453079.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(36909..38396)
                     /gene="nusA"
                     /locus_tag="J1138_001253"
     CDS             complement(36909..38396)
                     /gene="nusA"
                     /locus_tag="J1138_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBA8453080.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(38424..38876)
                     /gene="rimP"
                     /locus_tag="J1138_001254"
     CDS             complement(38424..38876)
                     /gene="rimP"
                     /locus_tag="J1138_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBA8453081.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         9272 a        10569 c         9990 g         9250 t
ORIGIN      
        1 gatttgcgct ttaccttgcc gggaggtact gcaatccctg aaatgattga cattgatcac
       61 atttctgcat ttaaactcct gacattctta tttcacccaa tgaagttatt tatttttaaa
      121 tgagaccagg tcctcatttt aatagtccct ggctggagaa tattgcacaa tggccaacat
      181 cgaaatcaga caagaaacgc caactgcgtt ttatataaaa gttcacgaca cagataatgt
      241 ggcaattatt gttaatgata atggcctgaa agcaggaacg cgttttccgg atgggctgga
      301 attaattgaa catattcccc aggggcataa agtcgcattg ctggacattc cggctaatgg
      361 tgaaattatt cgttatggcg aggtgattgg ttacgccgtg cgcgcaatcc cacgcggaag
      421 ctggatcgac gaatcaatgg ttgtactacc ggaagcgccg ccgttacata cgctgccact
      481 ggcaaccaaa gtcccggaac ccttaccgcc gctggaagga tacacctttg agggctatcg
      541 caatgccgat ggcagcgtgg gcaccaaaaa cctgctcggc atcaccacca gcgtccactg
      601 tgtggcaggc gtggtggact acgtagtaaa aatcattgaa cgcgatctgc taccgaaata
      661 cccgaacgtc gatggcgtgg tggggctgaa tcatttgtac ggttgtggcg tggcgattaa
      721 cgcacccgcg gcagttgtac ctattcgtac cattcacaat atttcgctga accctaactt
      781 tggcggcgaa gtaatggtga ttggtctggg ttgtgaaaag ttgcagcctg aacgcctact
      841 gactggaacg gatgatgtgc aagctattcc agtagaaagc gccagcattg tcagtttgca
      901 ggatgaaaag catgtcggtt ttcagtccat ggtcgaggat attttgcagg tcgccgaatg
      961 ccatctacaa aaactgaatc aacgtcagcg agaaacctgt ccggcttcag aactggttgt
     1021 cggcatgcag tgcggtggca gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg
     1081 gtatgcgtct gatctattgg tgcgctgcgg cgcaacggtg atgttttcag aagtcacaga
     1141 agtgcgtgac gcgatacatc tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg
     1201 gctgctggag gagatggagt ggtacgataa ctatctcaat atgggaaaaa ccgatcgcag
     1261 cgccaaccct tcgccgggga acaagaaagg cggtctggca aatgtggtgg agaaggcact
     1321 cggctccatt gctaaatcgg gtaaaagtgc aattgttgaa gtgctgtcgc ccggccaacg
     1381 cccgactaaa cgcggattaa tttacgccgc gacgccagcc agcgattttg tctgtggcac
     1441 gcaacaggtg gcttcgggta tcaccgtgca agtgtttacg accggtcgtg gtacgccgta
     1501 cggcctgatg gcggtacccg tcattaaaat ggcgacccgc accgagctgg cgaaccgctg
     1561 gtttgattta atggatatta atgcgggcac catcgctacc ggcgaagaaa ctattgaaga
     1621 ggtgggctgg aagttgttcc actttattct cgacgtcgcc agcgggaaga agaaaacctt
     1681 ctcagatcaa tggggactgc ataaccagct ggcggtgttt aacccggcac cggtgacctg
     1741 ataattcaat tcgtattatg cccgatagca cacgtttatc gggcatattg agtcacagcg
     1801 aagacgcgtg atccccgacc agaatcactt caaccccagc ctttctcagt ccttccagac
     1861 tatccgcagg aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct
     1921 tatgtaaact agaacgattg aacttactgg aatcggtgac cacgatgatc cgttccgcaa
     1981 cttcgcacat ccgacggttt aaacgggctt catcttcatt atgcgtgctg acgccgcgct
     2041 ccagatcgat cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt
     2101 gctcagcctg atcaccgtaa aaagattgcg actgacggcg caaatgcccc ccggtcatca
     2161 gcagctcaac gccttccgct tccagcaacg cattagccac gttcataccg ttggtcatcg
     2221 caattacgtc ggtgtgcttg cgcatcagac gagcaatctc aaaagtggtg gtcccggagt
     2281 cgaggatcac ccgatgacca ggctgaatca actcaacggc agctttcgca acgctgcgtt
     2341 tcatcgcggt gttcagcgcg cttttatctt ccactgatgg ctcgactgac ggcgttgtgc
     2401 tatcgcagat caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg
     2461 ccagatcgtt gcggatcgtc acagtagata caccatacaa cgccgataga tcattaactt
     2521 gcacacttcc ttgctggcgc aggcgttgaa tgatctgctc tcgtcgctca ctggtgcctg
     2581 tcactcgctt ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt
     2641 cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttac
     2701 ccattggcgc aagctactct cgtttcagtg gctttcatta tgtttctttt gtgaatcaga
     2761 tcagaaaacc attatctttc gttttatttt tatctcacca tgacgcagta tcaactgaaa
     2821 caaaacgaaa gattaatgtc gcagtaatct gaactggaga ggaaagtgaa acatctgaca
     2881 gaaatggtga gacagcacaa agcgggcaaa acaaatggga tttacgccgt ttgttccgca
     2941 catccgctgg tgctggaagc tgcaatccgc tacgccagtg caaaccaaac gccgttactg
     3001 attgaagcaa cctccaatca ggtggaccag ttcggcggtt ataccggaat gacgcccgcc
     3061 gattttcgcg gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgctg
     3121 attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgcctgc cgctcaggca
     3181 atggccaatg ccgatgattt gattaaaagc tacgtggcgg caggattcaa aaaaattcac
     3241 cttgattgca gcatgtcctg tcaggacgat ccgattccct taactgatga catcgtggct
     3301 gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcgtgaaca ctttggcgaa
     3361 gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc
     3421 ttaagcgagc tggcggtcac cacgccggat gccgcccgcg tcacgctgga agcccatcgt
     3481 cacgcctttg aaaagcaagg tctgaatgcc atctggccac gcatcattgc cctggtggtt
     3541 caacccggcg tcgaattcga tcacaccaac gttattgatt atcagccagc caaagccgct
     3601 gccttaagcc agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat
     3661 tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc actttgccat tctgaaagtt
     3721 ggcccggcgc tgaccttcgc cctgcgtgaa gctctgttct ctctggcggc gattgaagaa
     3781 gaactggtgc cagcgaaagc ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc
     3841 gaccgcccgg aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg
     3901 cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac
     3961 gctttcgctc atctggttcg taatctggcg gattcaccaa ttccgctgcc gctgatcagt
     4021 cagtatctgc cgctgcagta cgtgaaagtt cgctccggcg agctgcagcc aacgccacgg
     4081 gaactcatta tcaaccatat tcaggacatc ctggcgcagt accacacagc ctgtgaaggc
     4141 caataagcaa aacaaagagg aacacgctat gccaaatatt gttttaagtc ggattgatga
     4201 acgcttgatt cacggtcagg tcggcgttca gtgggtcgga tttgcggggg caaatctggt
     4261 gctggtagcc aacgatgagg ttgccgaaga tctggtacaa caaaacctga tggaaatggt
     4321 actggcagaa gggatcgccg tacgtttctg gacgctgcaa aaagtcatcg acaacattca
     4381 tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acaccggccg atttcctgac
     4441 gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa
     4501 tggcaagcaa caaatcgcca aaacggtttc tgtggatgcg ggtgatatcg cagcatttaa
     4561 cgacctgaaa gccgctgggg tggaatgctt cgttcagggc gtcccaacag agcctgctgt
     4621 ggacctcttt aaattacttt gagggattca tcatggaaat cagtctgttg caggcatttg
     4681 cgttgggcat tatcgccttt atcgctggcc tggatatgtt taacggctta acccatatgc
     4741 accgcccggt ggttctcggc ccgttggtcg ggctggtact tggcgatctg cataccggta
     4801 ttttaaccgg cggtacgctg gaactggtgt ggatggggct ggcccctctg gcgggcgcac
     4861 agccgcctaa cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg
     4921 tgaaacccga tgtcgcggtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga
     4981 ttaccttcct gttctcggtg atgtctggcg tgatgtcccg ctgcgaccgg atggcggaga
     5041 acgccgatac tcgcggcatt gaacgcgtga actatctggc actgctggca ctcggcactt
     5101 tctattttct ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca
     5161 ttgatgtcct gccgcaacga ttaattgatg gcctcggcgt cgcgggcggc atcatgccag
     5221 caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca
     5281 tcctgggctt tgttgccgcg gcctggctca agttaccggt gttggctatt gctgccgctg
     5341 cgctggcgat ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact caacctgcgg
     5401 cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa
     5461 aatgaagaaa cactgctgac tggcgtcaat gaaaacgtgt atgaagatca gagcattggc
     5521 gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgtt gttacaggct
     5581 tctttcaact acgaacgtat gcaggcttcc ggctggctgt acggcctgct gccagcactg
     5641 aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc
     5701 ttcaataccc atccgtttct ggtgacgttt gttatcggca ttatccttgc gatggagcgt
     5761 tctaagcagg acgttaacag tattcagagc accaaaattg ccgtcggtgc gccgctcggc
     5821 gggattggcg atgcgatgtt ctggctaacg ctactgccga tttgtggcgg gataggtgca
     5881 agcctcgcgc tacaaggctc tattcttggc gctgtcgtct ttattgtgct gttcaacgtg
     5941 gtacacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg
     6001 attccactca ttaaagcaaa taccaaaaaa gtcggccatg cggcatctat cgttgggatg
     6061 acggtaatcg gcgcgctggt ggcaacctat gttcgtttaa gcaccacgct ggaaatcaca
     6121 gcgggcgacg cagtggttaa gttacaggct gatgttatcg acaaactgat gcctgccttc
     6181 ttaccgctgg tctacaccct gaccatgttc tggctggtac gccgcggctg gagtccgctg
     6241 cgcctgattg cagtgacagt ggttctcggc atcgtcggta aattctgcca tttcctttaa
     6301 atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcggg tttgccagcg
     6361 gtatggaaaa agcaatgaag caaattctcg gtgagcaatc gcagtttatc gccatcgatt
     6421 ttccggaaac ctcaagcacc gcgcagctta catcgcagct tgaagaggcc atcgcccaac
     6481 tggattgtga agacggcatt gtttttctga cggatttgct gggcggcacg ccgtttcgcg
     6541 tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc acaaatttgc
     6601 aactattact ggaaatggtg ctggaacgcg aagggttaag cggtgaagag ttccgtgtgc
     6661 aggcgctgga gtgtggacac cgtgggctaa ccagtctggt ggacgaactt ggtcgctgcc
     6721 atgaggaatg tccggtcgag gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct
     6781 gactgaagag ggatggctcg atgaccatca gttgcgtatt gctgacggtg taatcgcagc
     6841 aatcgaaccg attccagtgg gcgtgactga acgcgatgtg gaactgctct gccccgctta
     6901 catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg cgccggatgc
     6961 gctcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt taccgaccac
     7021 cgtaaccgcg ccgcttaata ccattcatgc ggcgctgaaa cgtattgctc tacgctgcca
     7081 gcgtggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc
     7141 gcagaataaa ggcgcgcatc caccggagct gtttcgcgag cttgaaattg ccgagctgga
     7201 tcaattgatt gccgtttctc agcatacctt gcgcgtggta gcgctggcac cagaaaaaga
     7261 aggggcattg caggccatcc gccatcttaa acagcaaaac gtacgggtga tgctggggca
     7321 tagcgcggcg acctggcaac aaacccgtgc cgcgtttgat gctggtgccg acggcctggt
     7381 gcattgctat aacgggatga caggtttaca tcaccgcgaa ccgggaatgg ctggcgcggg
     7441 attaacggac aagcgcgcct ggctggaact gatagccgat ggtcatcatg tacatccggc
     7501 ggcaatgtcg ctctgttgtt gctgtgcgaa agagaggatc gtactgatca ccgacgcgat
     7561 gcaggcagcc gggatgccgg atggtcacta tacgttatgt ggtgaagaag tgcagatgcg
     7621 cggtggcgtt gtccgtaccg cgtccggtgg gctggcgggc agtacgctgt ctgtcgatgc
     7681 ggcagtgcgc aacatggtcg agttgacggg cgtaacgcct gcggaagcca tccatatggc
     7741 gtcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg
     7801 caaacgcgcc agcgtcgttg cgctggatag cgggctgcat gtgcaacaaa tctggattca
     7861 gggtcaatta gcttcgtttt gatggtttgc tcctttatta ggccttcact tcccccgtaa
     7921 ggcctttctt tttctttcgt tttgatctgt gcagcggtgt cggatgcgac gctaatgcgt
     7981 cttatccgac ctacggttgg tgaccgtaag gccggataaa gtatttacgc cgcatccagc
     8041 aatccctttt acttcccttg ccttttcttt caacgatcac agatttcgtt ttatttcctt
     8101 tttctccatt gaactttcag tttcttttct atagatttta accaacgaaa gaaatcacca
     8161 agtgaaatga aacgaaaggc aagcgaaagc gacaacgccg acgtcaagtt catcagacta
     8221 aggattgagt tatgccagaa aattacaccc ctgctgctgc cgcaaccggt acatggactg
     8281 aagaagagat ccgccatcag cctcgcgcat ggatccgttc actcaccaac attgacgcgc
     8341 tacgttccgc gctcaataac ttccttgaac cgttactgcg caaagagaat ctgcaaatca
     8401 tcctgaccgg agccggaaca tcggcattta tcggtgacat catcgcgccg tggctcgcca
     8461 gccataccgg taaaaacttc agcgccgtac cgaccaccga tctggtcacc aatccaatgg
     8521 actacctgaa cccagctcat ccgctactat tgatctcctt cggtcgatcc ggcaacagcc
     8581 cggaaagcgt cgccgccgtg aaactggcaa atcaatttgt accagaatgc tatcacctgc
     8641 cgatcacctg caacgaagcg ggcgctcttt accaaaacgc gatcaacagc gacaacgcgt
     8701 ttgccctgct gatgcccgca gaaacgcacg atcgcggctt cgcgatgacc agcagcatta
     8761 ccaccatgat ggccagctgc ctcgcggttt tcgcacctga gatgatcaac agccaaacct
     8821 tccgcgacgt ggcggatcgt tgccaggcga tcctgacctc actgggcgat ttcagcgaag
     8881 gtgtgtttgg ttacgcaccg tggaaacgga tcgtttatct cggcagcggt ggcttacagg
     8941 gcgcagcacg cgagtcggcg ctgaaagtgc tggaactgac agcgggtaaa ctggcggcct
     9001 tctatgattc cccgaccgga ttccgtcatg gcccgaaatc gctggtcgat aacgaaacgc
     9061 tggtggtggt gtttgtctcc agccaccctt acacccgtca gtatgatctt gatctgctgg
     9121 cagaactccg ccgcgataac caggcaatgc gcgtaatcgc catcgccgcg gaaagcaacg
     9181 acgttattgc cgccggtcca catatcatcc tgccgccgtc ccgtcacttt atcgacgttg
     9241 agcaggcatt ttgcttcctg atgtacgccc agacatttgc actgatgcag tcgctgcaca
     9301 tgggtaatac gccggatacc ccatcagcca gtggcaccgt taaccgcgtg gtgcaaggcg
     9361 taatcattca tccgtggcag gcataagagg atcgcattat gagcattatc tccaccaaat
     9421 atctgttaca ggacgcccag gccaatggct acgcggtgcc tgcctttaac attcataacg
     9481 ccgagacgat ccaggcgatc ctcgaagtgt gcagtgaaat gcgatcgccg gtgatcctcg
     9541 ccggaacgcc ggggaccttt aaacatatcg cgctggaaga gatctacgcc ctgtgtagtg
     9601 cctattccac aacctacaac atgccactgg cgctgcatct cgaccaccac gaatcgctgg
     9661 atgatattcg ccgtaaagtc cacgcaggcg tgcgcagcgc gatgatcgac ggcagtcatt
     9721 tcccgtttgc cgagaacgtg gagctggtga aatcggttgt cgacttctgc cactcccagg
     9781 attgcagcgt ggaagcagaa ctgggccgcc tcggcggtgt tgaagatgac atgagcgttg
     9841 acgccgaaag tgcattcctg actgatccac aagaagctaa acgctttgtc gaactgactg
     9901 gcgtcgacag cctggcggta gcgattggta cggcgcacgg cttatacagc aaaacgccga
     9961 agattgattt ccagcggctg gcggaaattc gtgaagtggt ggatgttcct ctggtgctgc
    10021 atggtgccag cgatgtcccg gatgaatttg tccgtcgcac tattgaactt ggcgtaacaa
    10081 aagtgaacgt tgccacagaa ttaaaaatag ccttcgctgg cgcggttaaa gcctggtttg
    10141 cggaaaatcc gcagggtaat gatcctcgtt attatatgcg cgtcggaatg gatgcgatga
    10201 aagaagttgt cagaaataaa attaatgtct gtggttcagc gaatcgaatt tcagcataat
    10261 cattagtttt ttatgattta tcccaatgta cttcccgatt aatatcgggg agtgccttaa
    10321 tggtaaagga gataactaaa ccttaataaa tacatcagta caatatcgca acaataatat
    10381 atttaaaaag attatattat tcaactttat ggtgaggatt acacaatgag cagtccaaat
    10441 attctcttaa cccgtattga taaccgtctg gttcacggtc aggttggcgt gacctggaca
    10501 tccaccatcg gtgcgaatct gttagtagtc gtggatgatg ttgtcgctaa cgatgatatt
    10561 caacagaaat taatgggtat taccgcagaa acctacggct ttggcattcg tttctttact
    10621 atcgaaaaaa ccattaacgt catcggcaaa gctgcaccac atcagaagat cttcctgatt
    10681 tgccgtacgc cacaaacggt acgtaaattg gtagaaggtg gtattgacct gaaagatgtc
    10741 aacgtcggca atatgcattt ctcggaaggg aaaaagcaaa tcagcagtaa agtttatgtc
    10801 gatgaccagg atctcacgga tttacgtttt attaaacaac gtggcgtaaa tgttttcatt
    10861 caggacgtcc ctggcgatca aaaagaacaa atccctgact aaatctaaaa tagccttaat
    10921 attggtttga ggtaataaaa atgcatgaaa taaccctact ccagggatta tccctggcgg
    10981 cgttagtttt ctttctgggg attgattttt ggctggaagc cttattttta ttccgcccga
    11041 taatcgtttg tacccttaca ggtgctattc tcggtgatat tcagactggc ttaattaccg
    11101 gtggcctgac agagttggct ttcgccggat taacccctgc aggtggtgtt cagccgccca
    11161 acccgattat ggcgggtctg atgaccactg tcattgcatg gtctacgggc gttgatgcca
    11221 aaacggcaat tggtcttggc ctgccgttta gtttgttaat gcagtacgtc attctgttct
    11281 tctattccgc tttctcatta tttatgacca aagccgataa atgcgcaaaa gaggcggata
    11341 cggcagcgtt ttcccgactt aactggacaa cgatgctcat cgtcgcttca gcgtatgcgg
    11401 tgattgcttt cctctgtact tacctggcac aaggggcgat gcaggcgctg gtgaaagcga
    11461 tgcccgcctg gctgacccac ggctttgaag tggctggcgg tattctgcct gctgttggtt
    11521 ttggcttgct gctgcgcgtg atgttcaaag cacaatatat cccttacctg atcgccggtt
    11581 tcctgtttgt ttgctacatc caggtcagca acctgttgcc ggttgccgta ctgggcgcag
    11641 gctttgcggt gtatgagttt ttcaatgcga aatcccggca gcaagcgcaa ccgcagcccg
    11701 ttgccagtaa aaatgaagaa gaggactaca gcaatgggat ctgaaatcag taaaaaagat
    11761 atcacccgtc tgggctttcg ttcgtcactg ctacaagcaa gctttaacta cgaaaggatg
    11821 caggcggacg gttttacctg ggcgatgttg ccgatcctga aaaagattta taaggacgac
    11881 aaaccgggcc taagcgcggc gatgaaagat aacctcgaat ttattaatac ccaccctaat
    11941 ctggtcggat tcctgatggg gttattaatt tcgatggaag aaaaaggaga aaaccgcgac
    12001 accattaaag gcctcaaagt ggcgctgttt ggcccaatcg ccgggattgg cgatgcgatt
    12061 ttctggttta ccttgttgcc aattatggcg gggatttgct catcgtttgc cagtcagggg
    12121 aacctgttgg gaccgatttt gtttttcgcc gtttacctgc ttatcttttt cctgcgcgtc
    12181 ggctggaccc acgtcggtta ttcagtcggc gtgaaggcta ttgataaagt gcgagagaac
    12241 tcgcagatga ttgctcgttc ggcaaccatc ctcgggatca cagtaatcgg cgggctgatc
    12301 gcttcgtatg tgcatattaa cgtggtgacg tcgtttgcca tcgacagtac ccacagcgtc
    12361 gcactgcagc aggatttctt cgataaagtc ttcccgaaca ttttaccgat ggcctacacc
    12421 ctgttgatgt attacttcct gcgggtgaaa aaagcgcatc cggtgctgtt aatcggcgtg
    12481 acttttgtgc tctctattgt ttgttctgca ttcggcattt tgtaaatgga acgaggcact
    12541 gcgtctggtg gtgcctcttt actaaaggaa tttcatcctg tgcaaaccct tcaacaagtt
    12601 gaaaactata cggcgttaag tgaacgtgcc agcgaatatt tattggccgt gatccgtagc
    12661 aaaccggatg ccgtgatttg cctggcgacc ggagccacgc cattactgac gtatcattat
    12721 ctggtagaaa aaatccacca gcagcaggtt gatgtcagcc atctcacctt cgtgaagctc
    12781 gacgaatggg tggatctgcc attaacgatg cctggcacct gcgaaacttt cctacaacag
    12841 catatcgtgc agccgctggg gctgcgtgaa gaccaactca tcagctttcg ctccgaagag
    12901 ataaatgaga cagagtgcga acgggtagcg aacctgattg cgcgcaaagg cggtctggat
    12961 ttatgcgttc tcggattggg gaaaaacggt catcttgggc tgaacgaacc gggagaaagc
    13021 ctgcaaccgg cctgccatat cagtcaactt gatgccagaa cacagcaaca tgagatgtta
    13081 aaaaccgcgg accgtcccgt gactcgtggg atcaccttag gcctgaagga tattctcaat
    13141 gcccgcgaag ttttgttact ggtgactggc gaaggaaaac aggatgcgac agaacgtttt
    13201 ctcacggcta aagtctccac cgctatcccg gcttcatttt tatggctgca taaccatttc
    13261 acatgcgtga ttgatgaaat gtgtcgtcgt taactcgcca ggtaaaggcg ttgtagcgct
    13321 attcctgctg cgacgcctgt aatcactctc cctgctgctc cagcgcatac ttatacagcg
    13381 catttttctt cacgccgtga atttctgcgg ccagcgccgc cgcttttttc agcggcagtt
    13441 ctgcctgtag cagcgccagc gtgcgcaggg catcagcagg caagtcattt tcctgcgctt
    13501 tatggccttc gacaatcagc accatttcgc ctttgcgacg gttttcatct tcttttaccc
    13561 acgccagcag ctcgccaacg ggcgcgccgt gaatggtttc ccaggttttg gttagctcac
    13621 gcgccagaac cacgtaacgg gattcgccta ataccgcaac aatatcttcc agactatcta
    13681 acagacggtg ggtagattca taaaaaatca gcgtgcgcgg ctccgcttca atagctttta
    13741 gcgcatcacg gcggcctttt gatttggcag gtaaaaagcc ttcataacaa aaacgatcgg
    13801 atggtaaacc cgcggcgctt aacgcagtga tagcagcaca cggccctggt aacggcacca
    13861 cgcgaatccc cgcttcacga caggtacgca ccagatggta gccaggatcg ttaattagcg
    13921 gcgttccggc atcggaaacc agcgcaatat tttgcccctc ttgcagcttc gccagcagcg
    13981 tttcggcttt ttgttgttcg ttatggtcgt gcagcgcaaa caaccgggca ttaatcccaa
    14041 aatgttgcag caataaaccg gtgtgacgcg tatcctcggc ggcaatcaga tcaacggcct
    14101 gtaatacctc taatgcacgc tgggtgatat ccgccagatt gccgattggc gtcggtacaa
    14161 tgtaaagctg cccctgagaa ttatccgccg attggtgttg tttcattgtg tcgtccgtat
    14221 tgccgattta atattgagca ttgcgtaaaa aaaatatcac tggatacatt atggtaccct
    14281 caacattttc tcgtttgaaa gccgcgcgtt gtctgcctgt tgttttggca gccctgattt
    14341 tcgccggttg tggcacccat actcccgatc agtccactgc ttatatgcag ggcacggcgc
    14401 aggctgattc tgccttttac ctgcaacaga tgcagcaaag ttctgatgat accaggatca
    14461 actggcaatt actcgccatt cgtgcactgg tgaaagaagg taaaaccgga caggcggtgg
    14521 agttgtttaa ccaactaccg caagaactga acgattctca gcgtcgcgag aaaacactgc
    14581 tggcggcaga gattaaactg gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga
    14641 aaatcacacc tgccgattta gaacaaaacc agcaagcccg ttactggcag gcaaaaatcg
    14701 atgccagcca ggggcgtcct tccattgatt tactgcgcgc gttaattgct caggaaccgc
    14761 tgctcggtgc gaaagaaaag cagcagaata ttgacgccac atggcaggcg ctctcctcca
    14821 tgactcagga gcaggcgaat acgctggtga tcaacgccga cgaaaatatt ctgcaaggct
    14881 ggctggattt gcagcgcgtc tggtttgata accgtaacga tcctgacatg atgaaagctg
    14941 ggatcgccga ctggcagaaa cgttatccga acaacccggg cgcgaaaatg ctgccaacgc
    15001 agttggttaa cgtaaaagcg tttaaaccag catcgaccaa caaaatcgcc ctgctgttgc
    15061 cgctgaatgg ccaggcagcg gtatttggtc gcactattca gcaaggcttt gaagcggcga
    15121 aaaatatcgg cactcagcca gtggcggccc aggtagcagc cgcacctgcc gcagacgtag
    15181 cagaacaacc tcagccgcaa accgtggatg gcgttgccag cccggcacaa gcttcggtta
    15241 gcgagctgac cggtgaacag cctgcggccc agccggtacc tgtaagcgcc ccggcgacaa
    15301 gcaccgcagc ggtaagcgca cccgcaaatc catccgcaga gctgaaaatc tacgatacct
    15361 catcacaacc acttagccag atcttaagcc aggttcagca ggatggtgcg agtattgtgg
    15421 tcggtccgtt gctgaaaaat aacgttgaag agttgctgaa gagcaacact ccgctgaacg
    15481 tgctggcact gaaccagccg gagaacatcg aaaaccgcgt caatatttgt tacttcgcgc
    15541 tttcaccgga agacgaagcg cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag
    15601 cgccgctggt gctgatccca cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc
    15661 aagagtggca gaaactgggc ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg
    15721 agttacgcgc gggtgttaac ggcggttcgg gtatcgcttt aacgggtagc ccgattactc
    15781 ccagagaaac aaccgactcc ggcatgacga ccaacaatcc aacgctgcaa accacaccaa
    15841 ccgatgatca gttcaccaat aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc
    15901 cgggtgaaat cgcttttatc aaaccaatga tcgccatgcg taacggtagc cagagcggtg
    15961 caacgctgta cgccagctcc cgcagtgcgc aagggaccgc tggcccggat ttccgcctgg
    16021 agatggaagg cttgcagtac agcgaaatcc cgatgctggc aggcggtaat ctgccgttaa
    16081 tgcagcaggc gctcagcgcg gtgaacaacg attattcgct ggctcgcatg tatgcgatgg
    16141 gcgtcgatgc ctggtcgttg gcaaatcatt tctcacaaat gcgccaggtt cagggttttg
    16201 aaatcaacgg taataccgga agcctgacgg caaacccgga ttgcgtgatt aacaggaagt
    16261 tatcatggct acagtaccaa caaggtcagg tagtccccgc cagttaacca ccaaacagac
    16321 cggcgatgcg tgggaagcac aagcgcgtcg ctggctggaa ggcaaaggac tacggtttat
    16381 cgccgctaac gtgaacgagc gtggcggcga gatcgatctg ataatgcgtg aaggccggac
    16441 caccgttttt atcgaggtgc gttaccgccg ctctgcgctt tatggcggcg cggcagccag
    16501 tgtgacccgc agcaaacaac acaaattatt acagactgcc cgcttgtggc tcgcgcgtca
    16561 taatgggagt tttgatactg tggattgccg gttcgatgtg gtagccttca ccgggaatga
    16621 ggttgagtgg attaaggatg cctttaatga ccactcataa ttaaggttta aggattagcg
    16681 tgcaagaaag aattaaagct tgcttcactg aaagcattca aactcaaatt gcggcggcag
    16741 aggcgcttcc ggatgccatc tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg
    16801 gcaacaaaat cctctgttgt ggtaatggaa cttccgctgc caatgcacag cattttgctg
    16861 ccagcatgat caaccgtttc gaaacggagc ggcccagctt acctgccatt gcactaaata
    16921 ctgataatgt tgtcttaacg gcaattgcca acgatcgctt acatgatgaa gtgtatgcaa
    16981 aacaggtgcg ggcgctgggc catgcgggag atgtattgtt agccatttcc acccgtggca
    17041 acagccgcga tattgtaaaa gcggtagaag ctgccgttac gcgtgatatg accattgtgg
    17101 cattaaccgg ctatgacggc ggtgaacttg caggtttgtt agggccacag gatgtggaga
    17161 tccgcattcc ttcgcatcgt agtgctcgca ttcaggaaat gcatatgctg acggtaaatt
    17221 gcctgtgcga tctgatcgat aacacgcttt tccctcacca ggatgtttaa ggagaataca
    17281 tgaaggcatt atcgccaatc gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg
    17341 ccgctgccgt agtgggtact gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg
    17401 tcggcaccca ggtagacgat ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag
    17461 acgaacaaat taagaaagaa gcgcgcatta atgtaacggc ctatcagggc aaagtgctgc
    17521 tggttgggca gtcaccaaac gctgaacttt cggctcgcgc caaacagatt gctatgggcg
    17581 tagacggtgc caacgaagtg tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg
    17641 aagcgtctaa cgatacgtgg atcaccacca aagtacgttc gcagctgtta accagcgacc
    17701 tggtgaaatc gtccaacgtg aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc
    17761 tggtgactga acgtgaagcg aaagcggcgg cagatattgc cagccgggta agcggcgtga
    17821 agcgggtaac tacggcgttt acgtttatta aatagcagcc cttgtaatgc ctgatgcgac
    17881 gcttgcagcg tcttatcagg cctacaacga tgcaaaccgt aggtcggata aggcgtttac
    17941 gccgcatccg acatgttttc cctcaaatca aaccaacagc gccagcccgc cgacaatcac
    18001 accagacact gccaccatcg cccccgtcag ccataaggct ttcgtcggga acgctttgcg
    18061 cagcataatc agtgacggca aactcaccgc cgggagcgtc atcaacaatg ccagcgccgg
    18121 agcagttccc atacctgcca gcatcatcgt ttgtacaatc ggaatttctg ctgccgtggg
    18181 aatgacaaac aagcatcctg ccaccgccat cgccaccacc cacatcaggc tgttatcgac
    18241 agcaccatcg gcatggggga ataaccagac gcgagcggca cccaacacca gtactgcaag
    18301 gatgtaaacc gggatcgtac tccagaaaag cgtccatagc gccctgcccc agcggctaaa
    18361 aaatcctccc tgtgcttccg gtatgtcaat ttcgaccggt gcctgcgttt gcggtgtttc
    18421 acgcacccat ttttgcacca gcgtcgcaat cagcaacacc atcaccagcc ccgccaccag
    18481 acgaatcgcc gcaaaatccc agccgaggac gaaccccata aacaccagcg tcgccgggtt
    18541 taacaacgga ttccccatcc agaatgccag cgcaccgccc atcgacacct gttggcgacg
    18601 cattcccgcc gcgaccggag ccgcacagca ggtacacatc atacccggca gcgaaaacag
    18661 cgttcccagc agcgtgccgc gaaagcgcga ttgcccaagc gtacgcaaca accagtcacg
    18721 cgggatcagc acctgaatca acgaaccgag gatcaccccc agcaccgccg ctttccatac
    18781 cgcgagaaaa tagatcatcg cgtaattcaa cgccgcctgc cacggattag catccgcctg
    18841 cgcaaggata gatttaccga tactgtgggt ttcggcagca gtaaacgctt tgccgtagta
    18901 aggttgccat ttcacatacc agagaccggc aatgacaacg agaaagaaaa gcgcgggttt
    18961 ccaccactga atgggcgttg ccgcctgaga tgaagactga ccagtcatag cattccccag
    19021 gaataagtta tgataattga gcgcgtgaat attacgctca ctatcaattc ttgggaataa
    19081 ttattcagct cttttgcgta attctgaaga gcttaaaatc gtcacacctt catgctcagg
    19141 ccgcatcgcc tctgccagca tgacccgcgc cacgtctctc gcatcaatgg atttccagtt
    19201 accgggtaac aaacggaaca gcggcgcaaa aagcgtttcg ttcatccgct gtttgctacg
    19261 atcgcccagt aacatcgacg ggcgagcaat ggtcaatttc ggccagttct gggcgattaa
    19321 tgcttcttcc atctcccctt tgacgcggtt atagaaaaac ggcgagtggg cattagcacc
    19381 catagcactg accaccagca tatgctgcgc accgagccgc cgcccggtta atgcggtatc
    19441 caccaccaac gtgtaatcgg catgaataaa cgcctctttg ctccccgctt ctcgccgcgt
    19501 ggtgccgaga caacaaaaca caatgtcgat gggatcggtg acctgcgcca gcgcgtcagt
    19561 cagttgcgga tcatggggat taaacacccc gggcatatcg cccaacggac gtcgcgtcgg
    19621 cgcagcaatg gcgttaactt tcggttcatt aatcaacatc cgcagcaggt gaccgcccac
    19681 cagccccgtt gcgcctgtaa tcagtacctg actcatcttc gctcctttac agaattgtct
    19741 gccttgcgct ccccggctca tgcaccaggc ttaatagatc atgaggtaat tatccccgat
    19801 tgtggaaaat tcgcctcatc caatgcaaca acgcggagga agcatgagta agaaaatcgc
    19861 cgttttaatc actgatgaat ttgaggattc agaatttact tcacccgcag acgagttccg
    19921 taaagccgga cacgaagtga ttaccattga aaaacaagcg ggtaaaacgg tgaaaggcaa
    19981 aaaaggagaa gccagcgtgg ccatcgataa atccatcgat gaagtgacgc ccgcagagtt
    20041 cgatgccctg ctgctaccgg gcggtcattc accagattat ctgcgtggtg acaaccgttt
    20101 tgtcaccttc acacgcgatt ttgtgaacag cggcaaacct gttttcgcca tctgtcacgg
    20161 tccccagttg ctgatcagcg ccgatgtgat ccttggtcgt aagctgaccg ccgttaaacc
    20221 gatcgtgatt gatgttaaaa acgcgggcgc ggaattttac gatcaggaag tggtggtcga
    20281 taaagatcag ctggttacca gccggacacc ggacgatctg ccagcgttta accgcgaggc
    20341 gttacgcctg ctcggtgcct gagttacgta gccaaatcat ttttttacca aagcccagcg
    20401 tgttgtcggt gaatttgatt tcatcgagcc ggatttccca taccggggcc gacagcattc
    20461 tggcaaccgg aaagcgacga ttgtacgcct tgcgtgcgag gtcgctttcc tcgccttcca
    20521 gtctgcggat ctcaccttta aactgcacgc cgcgaattaa cgctaccgtt ttcggctgac
    20581 cgtttaccgt tccggcaatt gccgcctgcg gcccgctcat ctgcgcatga cgcgtttttt
    20641 cttccgtcaa aatgtagaag gcgactttct gcgcatcaaa aagataaaag gcattggcgc
    20701 accacagttc cccttcctgc tgcacacacc aggtgacaac atgttgtttt gccagccaac
    20761 ggctgatggc ggtgagtgtt tccattgctg ttctctttta tactgtgggc ctgaccttaa
    20821 catgcgacgc agacgatgac accctggttt ctttacttga tccgtaccgc cgacaataag
    20881 ctttataccg ggatcaccac ggatgtcgaa cgccgctatc agcagcacca aagcggcaaa
    20941 ggggcgaaag cactgcgcgg gaagggagaa ctaacgctgg cgttttccgc accagtgggc
    21001 gatcgttcgc tggcgttacg ggcagaatat cgcgttaagc aactgaccaa acggcaaaaa
    21061 gagcgtctgg tagcggaagg tgcagtgttt gcagaactgt tgagcagcct gcaaaccccg
    21121 gagattaaaa gcgattgaag tgctcgtgat actcaaccag accagttaca ccgttcagcg
    21181 cgtcatccgc taaacgatgt acctggaagg cactttcagt gcccggccag cggcaacgca
    21241 gatcgtgatg tgccgccagt tcaaagccga aacggctgta cagcgccgga tcgcccagcg
    21301 tcaccactgc ggcatagccg aactcattaa gcgaatcgag tccttcatag accagttggc
    21361 gtgccagccc ctgtccacgg tatttttcat ctaccgccag cggtgccatg ccgacccatt
    21421 gcaggtcttc gccctgcaca tcaaccggac taaatgccac atagccaatg acctgacctt
    21481 cgtcatctgt cgccaccagc cccagcgtca gaaagccatc ttcacgcaga tcgtgaacca
    21541 gcttcgcttc cgcatcactt tcgaatgaac gacgcagcag ggcatcaata cccggcgcat
    21601 caatgggaat ttctactcga attagcatgg ttcacctacc gatgtctgtt tggtttcagg
    21661 cgcggttttc attcccgcct caacaaaatc cgccagttgc agcagcatca tgcgtaaggc
    21721 tttcggcatt tgctccagtt caatggcgtc catcaggttt ttcacataca gccccagctc
    21781 cgtatcgcct tcaatcacca gccgacgctg gaagaagagc gtatccggat cttgtttacg
    21841 cgccgcaatc atcagcagat cgctggcgtc ggcactaaaa ctcacatcag cttgcgcgtt
    21901 ctggctaacg accagtttgc cattcaccac cgaggtaaac cattgcaggt caatatcacg
    21961 cacatgaata cttaaccagc gaccttcaag aaactccagc tcgccatcat ccagcgcctg
    22021 acggaattgc cagcttaaga cctgctcaag aacctggcgt tttagcgcaa atggcgtcag
    22081 ttttaccggt acactcaaca gagatggccc caaatgcaca atacgggaac gcagtttatc
    22141 caacacgagt tttactccct gtttcaacaa tcatcctatt ttgccatatc agaaaaataa
    22201 catagcggta taaatcaaca attccatatg aaattgctgc taccaccaat acaactttaa
    22261 ctgccttaaa tcaaaaattg tcgcagcaag gttaactaaa atcccagttc gttaacattt
    22321 ttgcgttttg atagcgcaac cttcaggaaa aattatggag ctgctctgcc ctgccggaaa
    22381 tctcccggcg cttaaggcgg ccatcgaaaa cggcgcagat gctgtttata tcgggctaaa
    22441 agatgatacc aatgcccgtc acttcgccgg ccttaacttt accgagaaaa aattgcagga
    22501 agcggtgagt tttgtccatc aacatcgccg caaacttcac atcgcgatta acacttttgc
    22561 gcatccggac ggttacgccc gttggcagcg cgccgtggat atggcggcgc aactgggtgc
    22621 cgacgcgctg atcctcgccg acctcgccat gctggagtac gccgccgagc gttatccgca
    22681 tattgagcgc cacgtatcgg tgcaggcttc ggcgaccaat gaagaggcga ttaactttta
    22741 tcatcgccat tttgacgttg cccgcgtggt gctgccgcgc gtgttgtcga ttcatcaggt
    22801 gaaacaactg gcacgggtca cacctgtacc actggaagtc tttgctttcg gcagcctgtg
    22861 cattatgtcg gaaggtcgtt gctatctgtc gtcgtatttg acgggtgagt cgcccaacac
    22921 cgtgggcgcg tgttctccgg cccgtttcgt gcgctggcaa caaacgccgc aggggctgga
    22981 atcccgcctg aacgaagtgc tgatcgaccg ttatcaggac ggcgaaaacg caggttatcc
    23041 gacgctatgt aaagggcgtt atctggtgaa cggcgagcgc tatcacgcgc tggaagaacc
    23101 aaccagtctc aatacccttg agctgctgcc ggagttaatg gcggcgaata tcgcttcggt
    23161 gaaaattgaa ggccgccagc gtagcccggc gtatgtcagc caggtggcga aagtctggcg
    23221 tcaggctatc gaccgttgta aggccgatcc gcaaaacttc gtgccgcaaa gcgcgtggat
    23281 ggagacgctc gggtcgatgt ccgaaggcac gcaaactacc cttggcgcgt atcaccgtaa
    23341 atggcagtga gaaaagcaat gaaatattcc ttagggccgg tgctatggta ctggccaaaa
    23401 gagacgctgg aagaatttta tcagcaggcc gccgccagca gcgccgacgt gatttatctt
    23461 ggtgaagcgg tctgtagcaa acgccgggca accaaagttg gcgactggct ggagatggca
    23521 aaatcgctcg ccggaagtgg caagcagatt gtgctctcca cgctggcgct ggtgcaggca
    23581 tcctccgaac tgggcgaact gaaacgctat gttgagaatg gtgagtttct gattgaagcc
    23641 agcgatctcg gcgtggtgaa tatgtgcgct gagcgcaaac tgccgttcgt cgccggacac
    23701 gcgctgaact gctacaacgc ggtgacgctg aaaatattgc tcaaacaggg catgatgcgc
    23761 tggtgtatgc cggtggagct ttcccgcgac tggctggtga atctgcttaa tcagtgcgat
    23821 gagctgggca ttcgcaacca gtttgaagtg gaagtcctga gctacggtca tctgccgctg
    23881 gcctactccg cccgctgctt taccgcgcgt tcggaagacc gcccgaaaga tgagtgtgaa
    23941 acctgctgca ttaagtatcc gaacgggcgc aacgtgctgt cgcaggaaaa ccaacaagtg
    24001 tttgtgctca atggcattca gaccatgagc ggctacgtgt acaacctcgg taacgagctg
    24061 gcatccatgc aggggctggt tgatgtggtc cgcctgtcac cgcagggtac tgacactttc
    24121 gcgatgctcg acgccttccg cgctaatgaa aatggcgctg caccactccc gttgacggca
    24181 aacagcgact gtaacggcta ctggcgacgg ctggctgggc tggaactgca agcctaagca
    24241 aatagctcac tttgttaaca actttaacta ccctttaatg cagtattaaa gattaatcgg
    24301 taacaaagtg agctgttatg actgataaaa ccattgcgtt ttcactactc gatctggccc
    24361 ccatccccga aggttcttca gcgcgggaag cgttctccca ctctctcgat ctcgcccgtc
    24421 tggctgaaaa gcgcggctat catcgctact ggctggcgga acaccacaat atgaccggca
    24481 ttgccagtgc tgcaacatca gtattgattg gctatctggc ggcaaatacc accacgctgc
    24541 atctggggtc tggcggcgtg atgttaccaa accactcacc gttggtgatt gccgaacagt
    24601 tcggcacgct caatacgctc tatccggggc gaatcgattt ggggctgggt cgtgcgccag
    24661 gtagtgacca acggacaatg atggcactac gtcgccatat gagcggcgat attgataatt
    24721 tcccccgcga tgtcgcggag ctggtggact ggtttgacgc ccgtgatccc aatccgcatg
    24781 tgcgccctgt accgggctat ggcgagaaaa tccccgtgtg gttattaggc tccagccttt
    24841 acagtgcaca actggcggcg cagcttggtc tgccgtttgc gtttgcctca cacttcgcgc
    24901 cggatatgtt gttccaggcg ctgcatcttt atcgcagcaa cttcaaaccg tcggcacggt
    24961 tggagaaacc gtatgcgatg gtgtgcatca atattatcgc cgccgacagc aaccgcgatg
    25021 ccgaattcct gtttacctca atgcagcaag cctttgtgaa gctgcgccgc ggcgaaaccg
    25081 ggcaactgcc gccaccgatt caaaatatgg atcagttctg gtcgccgtcc gaacagtatg
    25141 gtgtgcagca ggcgctgagt atgtcgctgg tgggcgataa agcgaaagtg cgtcatggct
    25201 tgcagtcgat cctgcgcgaa accgacgccg atgagattat ggttaacggg cagattttcg
    25261 accaccaggc gcgcctgcat tcgtttgaac tggcgatgga tgttaaggaa gagttgttgg
    25321 gatagtatgt cttaacgcgg gaagccttat ccgagctggc aacgctgtcc tacgtaggcc
    25381 tgataagcga agcgcatcaa gcattgtgta ggcagcagaa atgtcggata aggcaccgct
    25441 gattactgat acaccggcag caaattaaag ctcgataaga tatgcaccag tgcgttgcca
    25501 acgccaaata ccagaatcag cataatcatc ggcttgccgc cccagacacg gaatttcggg
    25561 ctaccaaagc gtttacgaga tgcgcgggcc aacagcgccg gaacaattgc cgcccagatg
    25621 gtagccgcta agccagcata accaatggcg tacaggaatc cgttcgggaa cagaagcccc
    25681 cccacaacag gcggggcaaa ggtcagcaac gctgttttca agcggcccat agccgagtca
    25741 tcgaaaccaa acagatctgc cagatagtca aacaaaccca gcgtaacgcc gaggaacgaa
    25801 ctcgctaccg caaaatttga gaacacaacc agcagcagat ccaggctacg gctgttcagc
    25861 acgccgctta acgcctgtac cagcacatca atattaccgc ccttctcggc gatgccgata
    25921 aactccggac gagggatgtt gcccatcgtc gccagcaacc agatggtata cagcgccagc
    25981 gccatcagcg taccgtacac caggcatttc acgatggttt tcggatcttt gccgtaatac
    26041 ttcatcaggc tcggcacgtt accgtgataa ccaaaagaag ccagacagaa tggcagtgtc
    26101 atcagcagat acggcgcata agacgcattg ctttcggcga cgttgaacaa ggtcgttggc
    26161 tgtacatgcc ccagcaggct gccgaaggtg aggaagaaag taatgacttt cgcccccagc
    26221 acaattgctg tcatccggct gacagctttc gtactcaacc acaccacgaa cgctaccagc
    26281 agtgcaaaac caaatccagc tgcccgtgca gggacgttca gcgacatctc cgcgaaggtg
    26341 tgatgcagaa tcgaaccgct ggcagaaata taggcgtaag tcaggatata gagcacaaag
    26401 gcaatggaaa tgccgttgac tacgttccag cctttgccca gcaaatcttt ggtgatggtg
    26461 tcaaagctag aaccgattcg ataattcagg ttagcttcca gaatcatcaa gccggaatgc
    26521 agcatacaga accaggtaaa gatcagcgcc gccattgacc agaagaacca cgccccggac
    26581 atgaccacag gcagagaaaa catccctgcg ccaataatgg tgccgccgat aatcaccacg
    26641 ccgccaagca gcgacggtga cgtttgggtg gtggttagtg ttgccatgag ggcttctctc
    26701 cagtgaaaaa tagtgcgact gcgttgttat gtattgcact gtaccagtac atgagtacaa
    26761 aagacagaaa aaaagccccg atggttaaaa tcggggctgt atatattatt ttacagactg
    26821 tgttcgctgt tcagcgatga ttacgcatca ccaccgaaac gacgacgacc ggtagagtca
    26881 tcacgacgcg gtgcgcggcc ttcacgacgt tcgccgctaa aacgacgccc atcaccacga
    26941 cccccttcac ggcgttcacc gctgaagtta cgaccgcctt cacgacgttc gccaccgaaa
    27001 ccacgaccac cgccacgacg ctcaccgcca gtatgcggct gtgcatcgcc cagtaactgc
    27061 atgttcatcg gcttgttgag aatgcgagta cgcgtaaagt gttgcagcac ttcacccggc
    27121 atacctttcg gcagttcgat ggtggagtga gaagcaaaca gcttgatgtt accgatgtaa
    27181 cggctgctga tgtcgccttc gttagcaatc gcgccaacga tatgacgaac ttcaacacca
    27241 tcatcgcggc ccacttcaat acggtacaac tgcatatcgc caacatcacg acgttcgcga
    27301 cgcggacgat cttcacggtc gccacgcggg ccacggtcgt tacgatcgcg cggaccacgg
    27361 tcatcacggt cacggaattc acgtttcgga cgcatcggcg catctggcgg aacaatcaga
    27421 gtacgttcac cctgtgccat tttcagtagt gcagctgcca gagtttcgag atccagctct
    27481 tcaccttcgg cagtcggctg aattttgctc agcagtgcac ggtattgatc cagatcgctg
    27541 ctttccagct gctgctgtac tttagcggcg aatttttcca gacgacgttt gcccagcagt
    27601 tctgcgttcg gcagttcaac ttccggaatg gtcagcttca tggtgcgttc aatgttacgc
    27661 agcagacgac gctcacggtt ctcaacgaac agcagcgcac ggccagcacg gcccgcacga
    27721 ccagtacgac cgatacggtg aacgtaagac tcagaatcca tcgggatatc gtagttaact
    27781 accaggctga tacgctcaac gtccaggcca cgggccgcaa cgtcggtcgc aatcaggatg
    27841 tccagacgac catctttcag tcgttccagt gtctgctcac gcagcgcctg gttcatgtca
    27901 ccgttcagcg cggcgctgtt gtagccgtta cgctcaaggg cttctgccac ttccagagtc
    27961 gcgtttttgg tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc caggaaacgt
    28021 accagtgctt cgtttttacg cataccccag acagtccagt agctctggct gatgtcagga
    28081 cgggtagtca cgctggactg aatgcgcact tcctgcggct ctttcataaa gcggcgggta
    28141 atgcgacgaa tcgcttccgg catggttgca gagaacagag cggtctgatg accttccggg
    28201 atctgcgcca taatggtttc aacgtcttcg atgaagccca tacgcagcat ttcgtcagct
    28261 tcatccagaa ccagaccgct cagtttagag aggtccagag tgccacgttt caggtggtcc
    28321 agcagacggc ccggagtacc gacaacgatc tgcggcccct gacgcagggc gcgtaattgc
    28381 acgtcataac gttggccgcc gtacagggca accacgttta cgccgcgcat gtgtttagag
    28441 aaatccgtca ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc cagcaccaga
    28501 atctgtggtg ctttcagctc aggatcaaga ttctgcagca gaggtaaaga gaatgctgca
    28561 gtttttccgc tccccgtctg ggccataccc aggacgtcgc ggccattcag cagatgtgga
    28621 atacactctg cctgaattgg agatggtttt tcgtaaccca gatcgttaag ggcttcaagg
    28681 ataggagcct tcaggcccag atctgcaaaa gtggtttcga attcagccat gtagtacgtg
    28741 tgcctcaaaa ttaatggcgg ccagtctaca taactcatca tgaaattgat cagcaatttt
    28801 cattgaaaag tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac gccctcaccc
    28861 gttaaggtga tggcaatcaa aaaagattac gggctgatgt gtacgtcagc tattgctggt
    28921 ccgattctgc caggtcatct tggtcctggc ccaggagcga taattccaac aatgcgtatc
    28981 ggtgctcaac aaagttatga acgttgttgg caaccgccag tttgaacagt gccgtggcgc
    29041 tgtccaaatc ccccagactt aggtagtact tacctaaata gaagttggtt tcactgagat
    29101 gctcagcgag cgaggtgtta tccgttgcgt ccgccttgag cctttccatt aacgtttgtt
    29161 cgctaatgtt gcccaggtag aactcgacaa tgttccatcc ccactgttcc ttatccgatt
    29221 tctcgaagtg ctgtttcaac acttctttag cctgcttctc atcgagcttc tgctcggcga
    29281 gataaagcca cagactacgg aaaggatcat tgggatcgtc ttgataaaac gccagcagat
    29341 catcttgcgc taacttgtca cgaccgccgt aatataatgc gatcccgcga ttcaagtgcg
    29401 cgtagttgta agttggatca agctcaagta cagaatcaaa cgcttcatag gcagcatcaa
    29461 aattgcctgc ctgcgttaaa tatatgccta agtaattgaa tacttcaggc atatccggtc
    29521 ggattgccag cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg agactatcat
    29581 acaacactcc gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa gcccgactgg
    29641 caaggatttg ttccatacgt gccagaatca cttcctgctg taaagtcggt tgcaatggta
    29701 ccgcgaggac ttcactttta cgccaggaag tattactgca tcctgcaagc gtaagtgctg
    29761 tcgcaacgaa acaccagcgc aaaaaaggct tcatttccca ctcccgaaga ccacggttga
    29821 atgaacgtcc tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag ccccccgccg
    29881 cagcggaggg caaatggcaa ccttactcgc cctgttcagc agccggagct tccggtgctg
    29941 cagcaggttg agactgctca gtcgcttctt taatgctcag acggatacgg ccctggcgat
    30001 caacttccag aactttcacc ggtacttcct gacccatctg caggtaatcg gtcactttct
    30061 caacgcgttt atcagcgatt tgagagatgt ggaccagacc ttctttaccg ccaccaatgg
    30121 caacaaatgc gccaaagtca acgatacggg tcactttacc attgtagacg cggcccactt
    30181 cgatttctgc agtgatctct tcgatacgac gaatcgcgtg tttcgctttc tcaccgtcgg
    30241 tcgctgcgat cttcacagta ccgtcatctt caatttcgat ggtggtgccg gtttcttcgg
    30301 tcagagcacg gattacagaa ccgcctttac cgataacatc tttgatcttg tccgggttga
    30361 tcttgatggt atggatacgc ggtgcgaact cagagatatc gccacgcggc gcgttgatcg
    30421 cctgttccat tacccccagg atatgcagac gcgcaccttt agcctggttc agtgcaacct
    30481 gcatgatctc tttggtgata ccttcaattt tgatatccat ctgcaatgca gagataccgt
    30541 cacgggaacc cgcaactttg aagtccatat cgcccaggtg atcttcgtcg cccaaaatgt
    30601 cagacagtac aacgtagttg tcgccttctt tcaccagacc cattgcgata cccgcaacgg
    30661 cagctttgat cggcacacct gcgtccatca gcgccagaga cgcgccgcac acggaagcca
    30721 tagaagagga accgttggat tcggtgattt cagacacaac acgtacggtg tacgggaatt
    30781 tgtccatatc cggcattact gccagcacgc cgcgcttcgc cagacgaccg tgaccaattt
    30841 cacgacgctt cggagaaccg accatgccgg tttcgcctac ggagtacgga gggaagttgt
    30901 agtggaacag gaaggtatcg gtacgttcgc ccatcagttc atcaagaacc tgcgcgtcac
    30961 gagcggtacc cagcgttgcg gtaaccagcg cctgagtttc accacgggtg aacagcgcag
    31021 aaccgtgagt acgcggcagc acgccagtac gcacatccag accacgaatc atatcttttt
    31081 cacgaccgtc gatacgcggt tcgcctgcca gtacgcggct acgaacaacg tttttctcga
    31141 tagcgtgcag aatttcaccc agttcgtttt cgtccagggt ttcatcttca tcaagcagcg
    31201 tcgcgatggt ttcagatttg atgacatcaa cctgcgcata acgctcttgt ttgtcggtga
    31261 tgcggtaagc atcgctcaga cgagcttcag ccagtgcagc aacgcgcgcg ttcagcgctt
    31321 cgtttaccgg ctccggctgc cagtcccaac gcggtttacc ggcttctttc accagttcat
    31381 tgatgttctg aataacaacc tgctgttgtt catgaccgaa gactactgcg cccagcatct
    31441 ggtcttcgct cagcagttca gcttcagatt caaccatcag tacagcggct tcagtacccg
    31501 caacaaccag atccagtttg ctctctttca actcgtcctg agtcgggttc agtacgtact
    31561 ggtcattgat gtaacctacg cgggcagcac caatcggacc attgaacgga ataccagaca
    31621 gagacagtgc tgcggaagca ccaatcatcg cgacgatatc cgggttaact tgcgggttaa
    31681 cagaaaccac ggtggcgata acctgaactt cgttgacgaa gccttccggg aacagcgggc
    31741 gaatcgggcg gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttggg cggccttcac
    31801 gacggaagaa gctacccggg atacgaccag cagcgtaggt acgctcctga tagttaacgg
    31861 tcagtgggaa gaagtcctga cctggtttgg cttttttctg gccaacaacg gtaacgaata
    31921 ccgcagtgtc atccatgcta accataacag cggcagtagc ctgacgagcc atcatgccgg
    31981 tttccagagt cacggtgtgt tggccgtact ggaatttacg aacgatcgga ttaagcaatg
    32041 taatatcctt tctctttctt agacagtacc ttacggcact ggtgttaata cccgatcttc
    32101 tgcgcatcct cgcgactaat gacaacccta acccagctct atgtgggtaa agcctctcat
    32161 tagccgcgcg aacctctgca gcggaagatc attcatagca acaatacatt agtttccagt
    32221 gaattgctgc cgtcagcttg aaaaaagggg ccactcaggc ccccttttct gaaactcgca
    32281 agaattagcg acgcagaccc aggcgctcga tgagctgggt gtaacgtgct acgtctttac
    32341 gtttcaggta gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga ccacgacggc
    32401 tgtggtgatc ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc tgtgcagtca
    32461 gcagtgctac ctgaacttcg gtagaaccgg tgtcgtttgc gtcacgacca aactcagaaa
    32521 cgattttagc tgttgcttca gtacttagag acattttaaa actccaaagt atatagaatg
    32581 aaaggacgcc gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt gttaaacaat
    32641 ttacgcgacg ttaagcggca gtattctact cgtagcgacc tgttatcgca agacggttaa
    32701 cattacgccg ggtattcaac caccaggcga cgaggcgcga cgcggccttc atcgtcaatt
    32761 tcgcccatac cgataaactt gccgttctca ccttctgtaa cgcgaaccag tccttccagc
    32821 ggcgcaccgg atgtacgaac cgggttacca tttttgaagt aaacagaaga cgttaacgga
    32881 agattcacca ccgggtagtc cgaagctgga ctgtccattg gcatcagtaa tggatcaagt
    32941 aacttcgcgg ctggaatatc ctgctgttcg gcttgttcaa ccagttcacg cagatgttcc
    33001 agggtcacca tccgttcaac cggatattta cttaccgcca gacgacgcaa gtaaataaca
    33061 tgcgcgccac aaccgagttt ttcgccgaga tcgtcaatga tagtgcggat ataagtgcct
    33121 tttgagcagt gaatttccag ctccagctca ttgccttcat ggcgaataaa cagcaattca
    33181 taaacggtaa tcgggcgcgc ttcacgcgga acttcaatgc cctgacgcgc gtattcgtac
    33241 agttttttgc cctgatattt cagtgctgaa tacatcgaag ggatctgttc gatatcgcca
    33301 cggaaggtat ccagcgccgc cgccagttgc tctgcactaa aggttaccgg acgttcttca
    33361 acgatttgtc cgtcggcatc agaagtatcg gtacgctgtc caagacgcgc aatgacccga
    33421 taacgtttgt cggagtccag cagatactgg gaaaacttcg tcgcttcccc gaggcaaatt
    33481 ggcaacatgc cggtcgccag cgggtccagc gcaccggtat gcccggcacg gttggcgtta
    33541 taaatccgtt tcactttttg cagcgcatcg ttgctggaca tcccctgcgg tttatccagc
    33601 aacaaaacgc cgttaatgtc gcgaccgcga cgacgaggac gactcattag tcctccttgc
    33661 tgtcgtccgg gttaacacga cgttcttcgt catgtttgac cacgctggtc accaggtttg
    33721 acatgcgcat cccttcaacc agagagttgt cgtagaagaa ggtcagttcc ggcacgatac
    33781 gcaggcgcat cgctttcccc agcaggctgc ggatgaaacc agaagcttcc tgcaacgctt
    33841 tgatgcccgc tttaaccgcg tcttcatctt tgtcgttgag gaacgtcaca tatactttgg
    33901 catacgccag gtcgcgagac atttcgacac cggaaacggt ggtcatcatg cccagacgag
    33961 gatctttaat ttcacgctgc aggatgagag cgatctcttt ttgcatttcc tgcgctacgc
    34021 gctgcgggcg accaaattct ttcgccataa taaattctcc tgacaaaaaa ggggctgtta
    34081 gccccttttt aaaattaatt tcaggtggaa gggctgttca cgttggcctg ataagacgcg
    34141 ccagcgtcgc atcaggcaat ccatgcagga tgcggcataa acgccttatc ccgcatggaa
    34201 ccctaaaaac cttaagcgat ggtacgttgg atctcgatga tttcgaatac ttcgatcaca
    34261 tcgccagtgc ggacatcgtt gtagttctta acgccgatac cacattccat accgttacgg
    34321 acttcgttaa cgtcatcttt gaagcggcgc agggactcca gctcgccttc gtagataacc
    34381 acgttgtcac gcagaacgcg gatcgggttg tgacgtttaa ccacaccttc ggtaaccata
    34441 caacctgcga tggcaccaaa tttcggtgat ttgaatacgt cacgaacttc cgccagaccg
    34501 ataatctgtt gtttcagttc cggagacagc ataccgctca ttgccgcttt cacttcgtcg
    34561 atcaggttat agatgacgga gtagtaacgc agatccaggc tttccgcttc aatcacttta
    34621 cgtgcagagg catcagcacg tacgttgaag ccaaccagga tggcgttgga cgccgcagcc
    34681 agggtagcgt cggtttcggt gataccacct acgccagaac cgatgatctt cactttaact
    34741 tcgtcagtag acagtttcag caaggagtcg gagatagctt ccacagaacc ctgtacgtct
    34801 gccttcagga cgatgttcac ttcgtgaact tcgccttcgg tcatattggc gaacatgttc
    34861 tcgagtttag atttctgctg acgtgccagt ttaacttcgc ggaatttacc ctgacgatag
    34921 agtgcaactt cacgcgcttt cttctcgtca cgtacaacgg taacttcatc acccgcagcc
    34981 ggaacgccgg acaggccgag gatttccacc ggaatggacg gacccgcttc cagcacttcc
    35041 tgacccagtt cgttacgcat cgcacgaaca cgaccgtatt cgaagccaca cagaacgata
    35101 tcgcccttgt gcagagtacc ttcacgtacc agaacggtag caaccggacc acgaccttta
    35161 tcgaggaagg attcgataac cgcaccgctc gccatacctt tacgtaccgc tttcagctcc
    35221 agaacttccg cctgcagcag gatagcatcc agcagttcat caataccggt acccgctttt
    35281 gcagatacgt gtacgaactg gctttcgccg ccccactctt ctggcaggat gccgtactgg
    35341 gagagttcgt tcttaacgcg atccggatcg gcttctggtt tatcgatttt gttcacagca
    35401 accaccaccg gaacacccgc cgctttcgcg tgctggatag cttcgatggt ctgcggcatt
    35461 acaccgtcgt ctgcagcaac aaccaggact acgatgtccg ttgcctgcgc accacgtgca
    35521 cgcattgaag taaacgcggc gtgccccggg gtgtccagga aggtgatcat gccgttttca
    35581 gtttcaacgt ggtatgcacc aatgtgctgg gtaatgccgc ccgcttcgcc agaggccact
    35641 ttcgttgaac gaatgtagtc cagcagagag gttttaccgt ggtcaacgtg acccatgatg
    35701 gtcacaaccg gagcgcgcgg ttcagccgca gcacccgtgt cacggtcgct cattaccgcc
    35761 tcttccagct cgttttcacg acgcaggata actttgtggc ccatctcttc agcaaccagc
    35821 tgtgcggttt cctgatcgat aacctggttg atggttgcca ttgcgcccag tttcatcatc
    35881 gctttgatga cctgagagcc tttaaccgcc atcttgttcg ccagttcgcc aacggtgatg
    35941 gtttcaccga tcacaacgtc acggttaacg gcctgagcag gcttctggaa gccttgctgc
    36001 agcgaagaac ctttacgttt tccgccttta ccgccgcgta ctgctgcgcg tgcttcttca
    36061 cgatcagctt ttgattcagc gtgtttgttg cctttcttcg gacgcgctgc tttcgcgtta
    36121 cgaccacggc cacggccgcc ttcgacttca cgatcgcttt cgtcttctgc ctggcgagca
    36181 tgttgagaag tagtgacgtg ataatcgctg gaatcttcag tcggttccgc gttatcagtc
    36241 catttgtttt cttccgccat acgacgtgct tcttcagcaa cgcgacgtgc ttcttcttcg
    36301 agtttacgac gcgcttcttc ttcggcttta cgcttgagct ctgcagcttc ctgctcacgg
    36361 cgggcttttt cagcctgggc gtttttagtc atatcgtctt gttgattgct cactttgtct
    36421 ttttccgcag cttcacgttt cgcttgttca gcagcttcac gcttagcttg ttctgcggcc
    36481 tcacgttcag ctttttgttg cgcctcgcgt ttagccgatt cttctgcctc acgacgggct
    36541 tgctcttccg cttcacgctg cgcttgctct tccgctgcaa ggcgctcagc ctcttgcgga
    36601 tcgcgtttca caaaggtgcg tttcttgcgg acttcgattt gtaccgattt actttttcca
    36661 ccggtaccag gaatgttaag ggtactgcgt gttttacgtt gcagcgtcaa tttgtccggg
    36721 cctgaatttt tctgattcag gtggtcaatc aaagtctgtt tctcttgtgc agacacagag
    36781 tcgtcagcag acttccggat acctgcatca gcaaattgct gtaccaggcg ttccacggag
    36841 gtctgtcgct ctgcggccag cgttttaatc gttacatctg tcatgctgtt ccttcctgct
    36901 acagtttatt acgcttcgtc accgaaccag caaatattac gggcagccat aatcagtgct
    36961 ccggcttttt cgtcggtcaa cccttcgata tcagccagat catcaatgcc ctgttcggcg
    37021 agatcttcca gcgtacaaac gccacgggcg gccagtttga atgccaaatc acgatctacc
    37081 ccttcaaggt tcagcagatc gtcagccggt ttgttatcac cgaggctttc ttcctgggcc
    37141 tgtgcaatgg tggccagtgc atttttagca cgctcgcgca gtgcttcaac ggtcggctca
    37201 tcaaggcctt cgatttccaa cagctctttc atcggcacat aggccagttc ttccagcgtc
    37261 gagaagcctt cttctaccag aacagtcgcg aagtcttcat cgatatcgag atatttggtg
    37321 aaggtgtcga tcgctgcgtg cgcttccgcc tgatgcttag cctgcaggtc gtcaacggtc
    37381 atcacgttga gttcccaacc gctcagctgc gaagccagac gcacgttctg accgttacgg
    37441 ccaatcgcct gcgccaggtt accggcttca acggcgatat ccatggtgtg tttatcttca
    37501 tccaccacga tagaagcaac gtctgccggt gccattgcgt taatcacaaa ctgcgccggg
    37561 ttatcatccc acaggacaat atcgatacgc tcgccgccca gttcagtaga aaccgcctga
    37621 acacgcgcgc cacgcatacc tacgcaagca cctaccggat cgatacgttt atcgttggtt
    37681 ttcaccgcaa ttttcgcacg agatcccggg tcgcgagccg ctgctttaat ttcaatcact
    37741 tcttcgccga tttccggcac ttcaatacgg aacagttcga tcagcatttc cggcttggag
    37801 cgggtgacga acagctgcgc gccacgcgct tccgggcgaa cggaatagag cacgccacga
    37861 acgcggtcgc cagggcggaa gttttcacgc ggcagcatat cttcgcgcag gatcacggct
    37921 tcagcgttgt tgcccagatc cagagagatg ttgtcgcggt ttactttttt caccacgccg
    37981 gtgatgattt caccttcgtg ttcacggaac tgatcaacca ccattgcacg ttcggcttca
    38041 cgcactttct gcacgataac ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca
    38101 gactcaatct gatcttcaac gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg
    38161 gcttcaaggg tgatttcctt ggtcggctgg gtgacttcat caacaactaa ccagcgacgg
    38221 aaggtgtcaa aatcaccgct tttgcgatcg atctgtacgc gaacgtcgat ttcttgttca
    38281 tattttttct ttgttgctgt cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga
    38341 ggtagcgcct tttcattgga tacggcttca actacagcca aaatttcttt gttcatcgcg
    38401 ggcttttcac ctcatccaga ctattaaaag tggggaacca ggttcgcctt ctggatatta
    38461 ctcagcgcga acacttcatc tttaccttcg acggtaactg tgatcatttc accgtctacc
    38521 gctttgataa cgccctgcca tttacgacgg ttttgtaccg ccatacggag aaccagagtc
    38581 acctcttctc cgacaaaacg ggcgtagtgt tcagccgtga acagtgggcg atcgagaccc
    38641 ggtgaggaga cttccaggtt ataagcaacg gtgatggggt cttcaacatc cagcacagca
    38701 cttacctggt ggctcacatc agcacaatca tcaacattga tgccatcttc actatcaata
    38761 tagatgcgca gtgtggatgt gcgaccgcga ataaattcga tgccaaccag ttcaaaaccc
    38821 agggcctcaa ctggcgcagt aatcatctct gttaattttt gctctaatgt ggacaagccc
    38881 acccccaaga cataaaaaaa gggcctaaag cccagttatt ctgtagtcag ataacaaaaa
    38941 accccgataa atcggggctt tatataactg aaccctataa ccgcaactgc ggtctggagc
    39001 actttccaga aggatttttt caaatcccac tacgaaggcc gaagtcttca cagtatattt
    39061 gaaaaaggac tctaagggaa a
//
LOCUS       DADTFG010000025        38402 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.025, whole genome shotgun sequence.
ACCESSION   DADTFG010000025 DADTFG010000000
VERSION     DADTFG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.025"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(28..441)
                     /gene="hns"
                     /locus_tag="J1138_001255"
     CDS             complement(28..441)
                     /gene="hns"
                     /locus_tag="J1138_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBA8453082.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1045..1662
                     /gene="tdk"
                     /locus_tag="J1138_001256"
     CDS             1045..1662
                     /gene="tdk"
                     /locus_tag="J1138_001256"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBA8453083.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(1947..2994)
                     /locus_tag="J1138_001257"
                     /pseudo
     CDS             complement(1947..2994)
                     /locus_tag="J1138_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(3120..5795)
                     /gene="adhE"
                     /locus_tag="J1138_001258"
                     /gene_synonym="adhC"
     CDS             complement(3120..5795)
                     /gene="adhE"
                     /locus_tag="J1138_001258"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBA8453084.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            6272..6919
                     /gene="ychE"
                     /locus_tag="J1138_001259"
     CDS             6272..6919
                     /gene="ychE"
                     /locus_tag="J1138_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBA8453085.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(7077..7373)
                     /locus_tag="J1138_001260"
     CDS             complement(7077..7373)
                     /locus_tag="J1138_001260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453086.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRGWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            7657..9221
                     /locus_tag="J1138_001261"
                     /pseudo
     CDS             7657..9221
                     /locus_tag="J1138_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
     gene            9307..10227
                     /gene="oppB"
                     /locus_tag="J1138_001262"
     CDS             9307..10227
                     /gene="oppB"
                     /locus_tag="J1138_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBA8453087.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            10242..11150
                     /gene="oppC"
                     /locus_tag="J1138_001263"
     CDS             10242..11150
                     /gene="oppC"
                     /locus_tag="J1138_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBA8453088.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            11162..12175
                     /gene="oppD"
                     /locus_tag="J1138_001264"
     CDS             11162..12175
                     /gene="oppD"
                     /locus_tag="J1138_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBA8453089.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEAHK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            12172..13176
                     /gene="oppF"
                     /locus_tag="J1138_001265"
     CDS             12172..13176
                     /gene="oppF"
                     /locus_tag="J1138_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBA8453090.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(13229..13558)
                     /locus_tag="J1138_001266"
     CDS             complement(13229..13558)
                     /locus_tag="J1138_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBA8453091.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPAGVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(13593..15053)
                     /gene="cls"
                     /locus_tag="J1138_001267"
     CDS             complement(13593..15053)
                     /gene="cls"
                     /locus_tag="J1138_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBA8453092.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            15196..15369
                     /locus_tag="J1138_001268"
     CDS             15196..15369
                     /locus_tag="J1138_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBA8453093.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(15424..16677)
                     /gene="kch"
                     /locus_tag="J1138_001269"
     CDS             complement(15424..16677)
                     /gene="kch"
                     /locus_tag="J1138_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBA8453094.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(16977..17273)
                     /locus_tag="J1138_001270"
     CDS             complement(16977..17273)
                     /locus_tag="J1138_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBA8453095.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            17497..18213
                     /gene="tonB"
                     /locus_tag="J1138_001271"
     CDS             17497..18213
                     /gene="tonB"
                     /locus_tag="J1138_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBA8453096.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(18253..18651)
                     /gene="yciA"
                     /locus_tag="J1138_001272"
     CDS             complement(18253..18651)
                     /gene="yciA"
                     /locus_tag="J1138_001272"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBA8453097.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(18756..19295)
                     /locus_tag="J1138_001273"
     CDS             complement(18756..19295)
                     /locus_tag="J1138_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBA8453098.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(19325..20068)
                     /locus_tag="J1138_001274"
     CDS             complement(19325..20068)
                     /locus_tag="J1138_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBA8453099.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(20096..20185)
                     /locus_tag="J1138_001275"
                     /pseudo
     CDS             complement(20096..20185)
                     /locus_tag="J1138_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            20425..21078
                     /gene="ompW"
                     /locus_tag="J1138_001276"
     CDS             20425..21078
                     /gene="ompW"
                     /locus_tag="J1138_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBA8453100.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTEVKYKSNGAVLPAGRYSDNVRLDPWVFMFSAGYRF"
     gene            complement(21129..21935)
                     /gene="trpA"
                     /locus_tag="J1138_001277"
     CDS             complement(21129..21935)
                     /gene="trpA"
                     /locus_tag="J1138_001277"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBA8453101.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNAAD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(21935..23128)
                     /gene="trpB"
                     /locus_tag="J1138_001278"
     CDS             complement(21935..23128)
                     /gene="trpB"
                     /locus_tag="J1138_001278"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBA8453102.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLDLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(23140..24498)
                     /gene="trpCF"
                     /locus_tag="J1138_001279"
     CDS             complement(23140..24498)
                     /gene="trpCF"
                     /locus_tag="J1138_001279"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBA8453103.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLATVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFTNGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTREQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(24502..26097)
                     /gene="trpD"
                     /locus_tag="J1138_001280"
     CDS             complement(24502..26097)
                     /gene="trpD"
                     /locus_tag="J1138_001280"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBA8453104.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLKPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQRLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(26097..27659)
                     /gene="trpE"
                     /locus_tag="J1138_001281"
     CDS             complement(26097..27659)
                     /gene="trpE"
                     /locus_tag="J1138_001281"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBA8453105.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEN
                     LPQLSAENSCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            complement(27751..27795)
                     /gene="trpL"
                     /locus_tag="J1138_001282"
     CDS             complement(27751..27795)
                     /gene="trpL"
                     /locus_tag="J1138_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBA8453106.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            27933..28814
                     /gene="yciV"
                     /locus_tag="J1138_001283"
     CDS             27933..28814
                     /gene="yciV"
                     /locus_tag="J1138_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBA8453107.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            28811..29431
                     /locus_tag="J1138_001284"
     CDS             28811..29431
                     /locus_tag="J1138_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBA8453108.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            29459..31348
                     /locus_tag="J1138_001285"
     CDS             29459..31348
                     /locus_tag="J1138_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HBA8453109.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGHILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSTLLICFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGVLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            31561..32436
                     /gene="rluB"
                     /locus_tag="J1138_001286"
     CDS             31561..32436
                     /gene="rluB"
                     /locus_tag="J1138_001286"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBA8453110.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(32476..33066)
                     /gene="cobO"
                     /locus_tag="J1138_001287"
     CDS             complement(32476..33066)
                     /gene="cobO"
                     /locus_tag="J1138_001287"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBA8453111.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(33063..33821)
                     /locus_tag="J1138_001288"
     CDS             complement(33063..33821)
                     /locus_tag="J1138_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBA8453112.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEEIGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            34041..35090
                     /gene="sohB"
                     /locus_tag="J1138_001289"
     CDS             34041..35090
                     /gene="sohB"
                     /locus_tag="J1138_001289"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBA8453113.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(35126..35377)
                     /locus_tag="J1138_001290"
     CDS             complement(35126..35377)
                     /locus_tag="J1138_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBA8453114.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            35757..38354
                     /gene="topA"
                     /locus_tag="J1138_001291"
     CDS             35757..38354
                     /gene="topA"
                     /locus_tag="J1138_001291"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBA8453115.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         9661 a         9227 c         9812 g         9702 t
ORIGIN      
        1 tttttagcat gtgcaatcta caaaagatta ttgcttgatc aggaaatcgt cgagggattt
       61 accttgctca tccattgctt ttttgattac agctggagta cggccctggc cagtccaggt
      121 tttagtttcg ccgttttcgt caacgtagct atattttgcc ggacgctgag cacgcttagc
      181 tttggtgcca gatttaacgg cagcaaggct attcagcagt tcgttcgggt caataccgtc
      241 agcgatcagc atttcgcgat attgctgcag tttacgagtg cgctcttcaa cttcagcagc
      301 agccgcgctt tcttcttcgc gacgttcgtt aacaacaact tctaattttt ccagcatttc
      361 ttccagcgtt tcaagtgtac attctcttgc ctgcgcacga agagtacgga tgttgttcag
      421 aattttaagt gcttcgctca ttgtagtaat ctcaaactta tattggggtg gtttgttgag
      481 gtaataatag agccttaaat tcagttgtgc aatagccagg aatgtaagga attcaaaatt
      541 gttctttatt ttgtgccgcc aataaatatc ttttcataaa attagccaga aaagacgcgg
      601 catatagccc tatttgcacc gatgatttcg cagcacgtga ggttaaaact tcctgattca
      661 tgtcacattt tatggggaga taatcgtagg ctgacgacct ttcagtcttc tgtattagtc
      721 gtgtttacga gaattcccta ttaagcgaat gatgaaaagt agaacagtcg caataagagc
      781 agggacttag tattgcacta tctcctggag gtcaacagag ggctattaca tgcgcaacag
      841 gttaaagatt gtgaatagtt accggcagtc atttacccgc ttataacaag cgaggcagtt
      901 gtaatgatag ctcagaagga ttatgcaagg cttcgtaagg gagaacgcat ataccacttc
      961 tgtgcatact gttgagctga aaaactgacg aattatgata aactccagcc aactttattt
     1021 catatcattg agggcctgtg gctgatggca cagctatatt tctactattc cgcaatgaat
     1081 gcgggtaagt ctacagcatt gttgcaatct tcatacaatt accaggaacg cggcatgcgc
     1141 actgtcgtat atacggcaga aattgatgat cgctttggtg ccgggaaagt cagttcgcgt
     1201 ataggtttgt catcgcctgc aaaattattt aaccaaaatt catcattatt tgatgagatt
     1261 cgtgcggaac atgaacagca ggcaattcat tgcgtactgg ttgatgaatg ccagttttta
     1321 accagacaac aagtatatga attatcggag gttgtcgatc aactcgatat acccgtactt
     1381 tgttatggtt tacgtaccga ttttcgaggt gaattattta ttggcagtca atacttactg
     1441 gcgtggtccg acaaactggt tgaattaaaa accatctgtt tttgtggccg taaagcaagc
     1501 atggtgctgc gtcttgatca agcaggcaga ccttataacg aaggtgagca ggtggttatt
     1561 ggtggtaatg aacgctacgt ttctgtatgc cgtaaacact ataaagaagc gttagaagtc
     1621 ggctcattaa cggctattca ggaaaggcat cgccacgatt aataagaatt tctttactga
     1681 cagggtgagc agggcgcttt tatcctgtca gttcgtttta cgcacttctt ccgggctata
     1741 cacccttctc ggcagttttt taacgccgct atacgcctca cagagcgctt atacaccgac
     1801 attgacttgt aacctgtaaa gtacaatatc gctgtgtgta ggcgttatac atcgtcgcaa
     1861 atatgatgaa ggacaatgct gccggtttat ggaaaagttg ttttgggaaa acaaaaaacg
     1921 gccccagaag gggtaatgcc agtcagttaa gcaactgact ggctcttttt cggggctgtg
     1981 gggtatttcc agggcctctc ctttaccact ctcgggaagg ccctttccct tcttgtcggt
     2041 aatttcactg gttgtcccat acttgcaaga tcgcgcatca gctccggtat acgtcccggg
     2101 gaagcgtatg gcgtccgtca tcgacgtcag cagatggcag acttttcctt tgcgcgtcac
     2161 ggtcagcagg cgggctgtca cctcatttcc cagtcccggc cacttttttc gtgcctgcgg
     2221 gctgatgttc agcttcacca gatgatcgcc tttacccagt tttctgatct cttcatattg
     2281 cgctcccttt ctgagaggga tcatccagtg gcggtgttct cccgccaggc tccaggtatt
     2341 taacagtcct ggtgagtaat aacctttatc cattaacgtc agagtgttat cgccggtttg
     2401 ttctataagt tgctcagcaa gctcattttc gctgttcttc atcgtgccga aggctgcagt
     2461 cgtcagcaga tggctggtca gttccatctg gcagaccatt ttgacctgcg ggtagagcgc
     2521 cgagttcccg gcatgtgtct ggcgggggaa ggctgcatcg ttctctggtg tatccggtgt
     2581 gcgccagaac acaccatcga tggccagcag ggtcaggccg caccagtgcg gatgcggcgt
     2641 ggcgttatgc cagagctgcg ctgttttcgt gaacacgcgg cggacagcct caattcccag
     2701 acgctggcgg gtctgaataa cggcactggg ggcaacgaag gggcgattgc ccggcagcat
     2761 gatgtccagg cgattcgcaa tctggtgaag aggttcttta cgctcaagcg ccatgccaac
     2821 aatacaccag accatcattt cgaggggaag acggcgcttg tgtagcgtta cagtacctga
     2881 ttcggcaagg caacgagaga tgagttcggg ggcgaggtaa tcccccagag aagtcagtgg
     2941 gttacgcaga gaatcgtaac gggataccaa atcaagggcc tgtccaatga gcataaaaaa
     3001 atccggaaac gagtgagcgt ttccggattc ttacacagcc actggatcgg tcaactgatc
     3061 cttaactgac tggcattacc ccttctgggg ccgtttatgt tgccagacag cgctactgat
     3121 taagcggatt ttttcgcttt tttctcagct ttagccggag cagcttcttt cttcgctgca
     3181 gtttcgcctt ctacataatc acgaccgtag taggtatcca gcagaatctg tttcagctcg
     3241 gagatcagcg ggtaacgcgg gttagcgccg gtgcactggt catcgaatgc atcttcagac
     3301 agtttatcca cgttcgccag gaagtctgct tcctgaacgc cagcttcacg gatagatttc
     3361 ggaataccca gttcagcttt cagcgtttcc agccatgcca gcagtttctc gatcttagct
     3421 gcagtacggt cgcccggtgc gctcagaccc aggtggtcag caatttcagc ataacgacgg
     3481 cgagcctgcg gacggtcata ctggctgaat gcagtctgct tggtcgggtt gtcattcgcg
     3541 ttgtagcgaa taacgttaca aatcagcagg gcgtttgcca gaccgtgcgg aatatggaac
     3601 tgggaaccca gtttgtgcgc cattgagtga catacaccca ggaaggcgtt cgcaaacgcg
     3661 atacccgcga tagtcgctgc actgtgaaca cgttcacgcg ctaccggatt tttagaccct
     3721 tcgtggtagg acgctggcag atattctttc agcagtttca gtgcctgcag agcctgacca
     3781 tcagagaact cagatgccag tacagaaaca taagcttcca tggcgtgagt tactgcgtcc
     3841 agaccaccga aagcacacag ggacttcggc atgtccataa ccaggttggc gtcgacaatc
     3901 gccatatccg gggtcagtgc atagtctgcc agcggatatt tctgaccagt agtgtcgtca
     3961 gttacaaccg caaacggagt gacttcagaa cctgtaccag aagtggtggt gacagcgatc
     4021 attttcgctt tcacacccat tttcgggaac ttgtagatac gtttacggat atccataaag
     4081 cgcagcgcca gttcttcgaa gtgagtttcc ggatgttcgt acataaccca catgatcttc
     4141 gcagcgtcca tcggggaacc accacccagc gcgataatca cgtctggttt gaaggagttt
     4201 gccagttctg cacctttacg aacgatgctc agggtcgggt cagcttctac ttcgaagaag
     4261 acttcagttt caacgcctgc tgctttcaat acggaagtga tctgatcagc atagccattg
     4321 ttgaacagga agcggtcagt cacgatgagc gcacgtttgt ggccatcagt aatcacttca
     4381 tccagcgcga ttggcaggga gccacggcgg aagtagatag atttcggaag tttgtgccac
     4441 aacatgtttt cagctcgctt agcaacggtt ttcttgttga tcaggtgttt cggaccaacg
     4501 ttttcagaga tggagttacc accccaggaa ccacaaccca gagtcaggga aggtgcgagt
     4561 ttgaagttat acaggtcacc gataccaccc tgagaagccg gggtgttaat caggatacga
     4621 gccgttttca ttttctgacc gaagtaagaa acgcgagccg gttggttatc ctggtcagtg
     4681 tacaggcaag aggtatgacc gataccgccc atagcaacca gtttctctgc tttttctacc
     4741 gcgtcttcga aatctttagc acggtacatt gccagagtcg gggacagttt ttcatgtgcg
     4801 aacggttcgc tttcatcaac aacggtcact tcaccgatca gaatcttggt gttttctggt
     4861 acagagaagc ctgccagttc agcaatttta taggctggct gaccaacgat agccgcgttc
     4921 agcgcaccgt ttttcaggat aacgtcctga acagctttca gctctttacc ctgcaacaga
     4981 tagccgccgt gggttgcgaa acgttcacgt actgcgtcat aaacagagtc aacaacaaca
     5041 acagactgtt cagaagcaca gattacaccg ttgtcgaagg ttttggacat cagtacagat
     5101 gcaactgcac gtttgatatc agcagtttca tcgataacaa ccggagtgtt gcccgcgcct
     5161 acgccgatag ctggtttacc ggagctgtat gcggctttaa ccatgcccgg accaccagtc
     5221 gcgaggatca ggttgatgtc tgggtggtgc atcagtgcgt tagacagctc aacagaaggt
     5281 tgatcgatcc agccgatcag atctttcgga gcaccggcag cgatagcagc ctgtagaacg
     5341 atatcagccg ctttgttggt tgcatctttt gcacgcgggt gcggggagaa gataatggcg
     5401 ttacgggtct tcaggctgat cagcgatttg aagatagcag ttgaagtcgg gttagtggtc
     5461 ggaacgatac cgcaaataat accgatgggt tcagcgatag tgatggtacc aaaagtgtcg
     5521 tcttcagaca gaacaccaca ggttttttca tctttatagg cgttgtaaat atattcagaa
     5581 gcaaagtggt ttttgatcac tttatcttcg acgataccca tgccggattc ggcaacggcc
     5641 attttcgcga gtgggattcg agcatctgca gcagccagag cggcggcgcg gaagattttg
     5701 tctacttgct cttgagtgaa actggcatat tcacgctggg ctttttttac acgctctacg
     5761 agtgcgttaa gttcagcgac attagtaaca gccataatgc tctcctgata atgttaaact
     5821 tttttagtaa atcatctgct cgaatacgag agtatagtca gtgcggtgat gatttgctta
     5881 accgatggaa atcaaaagct tactcgcgct cacactcact gtgatttact aaaagagttt
     5941 aaacattaga gttattatct ctaatgcgtc acttccaggt ggcgtaagca agattactca
     6001 cttctgggta ctgattacgt gatccaaatc aaaattttgc aaagctgaca cctttcagca
     6061 tggcttttcg ccattatagc taacagttaa taaattgtag tatgatttgt tggctacatt
     6121 agcatgtttt gcacaactag gtaacaataa cgaatgatag caattttaag tagttaggag
     6181 gtgaaaaatg ctgtcaaaag gcgtattgtc agcgcgtctt ttcaacctta tttatggcta
     6241 acattatccg gcttttgctt cggagctaac cgtgattcag accctttttg attttcccgt
     6301 ttacttcaaa tttttcatcg ggttatttgc gctggtcaac ccggtaggga ttattcccgt
     6361 ctttatcagc atgaccagtt atcagacagc ggcagcgcga aacaaaacta accttacagc
     6421 caacctgtct gtggccatta tcttgtggat ctcgcttttt ctcggcgaca cgattctaca
     6481 actttttggt atatcaattg attcattccg tatcgccggt ggcatcctgg tggtgacaat
     6541 agcgatgtcg atgatcagcg gcaagcttgg tgaggataaa cagaacaagc aagaaaaatc
     6601 agaaaccgcg gtacgtgaaa gcattggtgt ggtgccattg gcgttgccgt tgatggcggg
     6661 gccaggggcg atcagttcta ccatcgtctg gggtacgcgt tatcacagca ttagctatct
     6721 gtttggtttc tttgtggcta ttgcattgtt cgctttatgt tgctggggat tgttccgcat
     6781 ggcaccgtgg ctggtacgag ttttacgcca gaccggcatc aacgtgatta cgcgtattat
     6841 ggggctattg ctgatggcgt tggggattga atttatcgtt actggtatta aggggatttt
     6901 ccccggcctg cttaattaat tcctttcaaa tgaaacggag ctgccatgct ccgtttactt
     6961 cgtcattatt tttactttgt tcctgcgcag ttatcaaaag caaaaggaat aggtaaaaat
     7021 attcttctca aattacagtt agttataggg atttccttaa ctgcttctcc tcaccatcat
     7081 gttattttcg ccacatcata atcctgggct tgctgaagaa taattgaaat gatattagta
     7141 attccactgc ctttggtaga ggaaagtgct aaataataat caattgttaa attattgtgc
     7201 atttcactac tggaactgta atcagaaaag agagacatgc ttaaccaacc tctatttgat
     7261 tgaattgaaa gatgtttgtt aaggcatgga tgcaagctat agattctgat acggtcaata
     7321 aaagagaatt gcttaacaat tttgcaaaat gtattggcga gtaagaaccg catttggtac
     7381 tttccgggca accgccaggc gattctttat tggtaatgag aataattaac aattaaagat
     7441 cgtcgcgaaa gaataatgtg tctcgacagg ggagacacag tacgaatcga cataaggtga
     7501 tcgtctgaat caccagaata aataaagtcg gtgatagtaa tacgtaacga taaagtaacc
     7561 tgacagcaga aagtctccga gcctgtgcag ggtcccaatc cgggattaca catgctggtt
     7621 aataccagta attataatga gggagtccaa aaaacaatga ccaacatcac caagagaagt
     7681 ttagtagcag ctggcgtttt ggctgcgctt atggcaggga atgtcgcgct ggcagctgat
     7741 gtacccgcag gtgtcacact ggcggaaaaa caaacgctgg tacgtaacaa tggttcagaa
     7801 gttcagtcat tagatccgca caaaattgaa ggtgttccgg agtctaatat cagccgagac
     7861 ctgtttgaag gcttactggt cagcgatctt gacggtcatc cggcacctgg cgtcgctgaa
     7921 tcatgggata ataaagacgc gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg
     7981 tctgatggca cgccagttac agcacaagac tttgtgtata gctggcaacg ttctgttgat
     8041 ccaaacactg cttctccgta tgccagttat ctgcaatatg ggcatatcgc cggtattgat
     8101 gaaattcttg aagggaaaaa accgattacc gatctcggcg tgaaagctat tgatgatcac
     8161 acattagaag tcaccttaag tgaacctgtt ccgtacttct ataaattact tgttcaccca
     8221 tcaacttcac cggtgcctat accttaaaag attgggtcgt aaacgaacga atcgttcttg
     8281 aacgcagccc gacctactgg aacaacgcga aaaccgttat taaccaggta acctatttgc
     8341 ctattgcttc tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc gacatgactt
     8401 ataacaacat gccgatcgaa ttgttccaga agctgaaaaa agagatcccg gacgaagttc
     8461 acgttgatcc atacctgtgc acttactatt acgaaattaa caaccagaaa ccgccattta
     8521 atgacgtgcg tgtgcgtacc gcactgaaat tgggtatgga ccgcgatatc attgttaata
     8581 aagtgaaagc gcagggcgac atgcccgcct atggttacac tccaccatat actgatggcg
     8641 caaaattgac tcagccggaa tggtttggct ggagccagga aaaacgtaac gaagaagcga
     8701 aaaaactgct ggctgaagcg ggttataccg cagacaaacc gttgaccatc aacctgttgt
     8761 ataacacctc cgatctgcat aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa
     8821 acattggtgt aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc gacacccgtc
     8881 accagggcac ttttgatgtg gcccgtgcag gctggtgtgc agactacaac gaaccaactt
     8941 ccttcctgaa caccatgctg tcgaacagct caatgaatac cgcgcattat aagagcccgg
     9001 cctttgacag cattatggcg gaaacgctga aagtgactga cgaggcgcag cgtacagctc
     9061 tgtacactaa agcagaacaa cagctggata aggattcggc cattgttcct gtttattact
     9121 acgtgaatgc gcgtttggtg aaaccgtggg ttggtggcta taccggcaaa gatccgctgg
     9181 ataataccta cacccggaat atgtacattg tgaagcacta atggcaatac gtggggcagg
     9241 agtgtcctgc tccacggtgt ctgattttta tagcattaca gaaggcacag gccagaaggt
     9301 agggcaatgt taaaatttat tctacgtcgc tgtctggaag cgattccgac gctatttatt
     9361 cttattacta tttcgttctt tatgatgcgc ctcgctccgg gcagtccttt taccggcgaa
     9421 cgtactttac caccggaagt gatggccaat atcgaagcga aatatcatct taatgatccg
     9481 atcatgacac agtatttcag ctacctgaaa caactggcgc atggcgattt cggtccatcg
     9541 tttaaatata aagattattc ggtcaatgac ctggttgcat ccagttttcc cgtttctgcc
     9601 aaactgggag ccgcagcatt ttttcttgcg gtaatactgg gtgttagtgc tggcgttatt
     9661 gccgcattaa agcaaaacac caaatgggac tataccgtga tggggctggc aatgaccggg
     9721 gttgttatcc ccagttttgt ggttgcgcca ttattagtca tgatatttgc gatcattttg
     9781 cattggctgc cgggcggtgg ctggaatggt ggagcgctta aattcatgat attgccaatg
     9841 gtggcgttat cactcgccta tatcgccagt attgcgcgta ttacccgtgg ctctatgatt
     9901 gaggttttac actccaactt tattcgtact gcccgggcga aagggttacc tatgcggcgg
     9961 atcattttac gccacgcatt aaaacctgct ctgttacccg tgctctccta tatggggccc
    10021 gcatttgtcg gcattattac cggttcgatg gtcatcgaaa ccatttatgg tttaccgggg
    10081 attgggcaat tgttcgttaa tggcgcattg aaccgtgact attccttagt gttaagtctg
    10141 accattctgg ttggcgcgtt aaccattttg tttaatgcca ttgtcgatgt gctatatgcg
    10201 gttatcgacc cgaaaatccg ttactgatac tggagctcgc gatgatgtta agtaagaaaa
    10261 acagcgagac gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc
    10321 aggacgcacg tcgacgtttt atgcataacc gtgcggcggt tgccagtctg atagtgctgg
    10381 tgctgatcgc gttatttgta atcctggcac cgatgctttc gcagtttgcc tatgacgata
    10441 ctgactgggc gatgatgtcc agcgccccgg atatggagtc cggtcactac tttggtactg
    10501 actcatccgg tcgcgacctg cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg
    10561 tcggtgttgc tgcggcactg gtggcagtgg tcgtggggac actttatggt tcgctttccg
    10621 gttatctggg cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc ctcaactcct
    10681 tcccattcat gttcttcgtc attttgctgg tgaccttttt cggtcaaaac atcctgctga
    10741 ttttcgtggc gattggcatg gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa
    10801 ccctgagtct gaagcgcaaa gagtttattg aggcggcaca agttggcggt gtatcgacgc
    10861 cgggcattgt tattcgccac attgtgccga acgtactcgg tgtggtggtg gtctatgcgt
    10921 cgcttctggt gcccagcatg atcctctttg aatctttcct tagcttcctg ggattaggta
    10981 cgcaagagcc gttaagcagc tggggtgcat tgctgagtga tggcgcgaac tcgatggaag
    11041 tctctccatg gttactgttg ttccctgcgg gattcctcgt ggtaacgctg ttttgtttca
    11101 actttatcgg cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc
    11161 catgagcgta attgaaactg caactgtgcc gctcgcacaa caacaggctg acgcactgct
    11221 gaacgtgaaa gatttgcgtg tcacctttag taccccggat ggcgacgtca cggcggtcaa
    11281 tgatttgaat ttttcgctac gtgccggaga aacgctgggc atcgtaggtg agtctggttc
    11341 gggcaaatcg caaaccgcat ttgcgttgat gggcctgttg gctgccaacg gacgtattgg
    11401 cggatcggca accttcaatg ggcgtgaaat cctcaatttg ccagagcgtg aactcaataa
    11461 actgcgagct gaacaaatct caatgatttt tcaggaccca atgacttcgt tgaatcccta
    11521 tatgcgcgtc ggtgagcagt tgatggaagt gctgatgctg cataagaata tgagcaaagc
    11581 tgaggcgttt gaagaatcgg tgcggatgct cgatgcggta aaaatgccgg aagcgcataa
    11641 acgcatgaaa atgtacccgc acgaattttc tggcggcatg cgtcagcgag tcatgattgc
    11701 gatggcattg ctatgtcgac ctaagctgct gattgcggat gaacccacta ctgcgctgga
    11761 cgtcaccgta caggcgcaga tcatgacgct attgaatgaa ctgaagcggg aatttaatac
    11821 cgccatcatt atgattaccc acgatcttgg cgtggtggcg gggatctgcg acaaagtgct
    11881 ggtaatgtac gccgggcgca cgatggaata tggcaacgcg cgcgatgtct tttatcaacc
    11941 cgtacatcct tattctatcg gtttgctcaa cgcggtgccg cgtctcgatg cggaaggtga
    12001 aacaatgttg actattccgg gtaatccgcc aaacctgctg cgattaccga aaggttgccc
    12061 gttccagcca cgttgtccgc atgcgatgga aatttgtagt agcgcaccgc cgctggaaga
    12121 gtttacgcct ggccgtctgc gtgcttgctt taaaccagtg gaggaactgt tatgaatgct
    12181 gtaaccgaag gaagaaaagt cctcctcgaa atcgccgatc tgaaagtgca ctttgaaatc
    12241 aaagatggca aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgta
    12301 actcttcgcc tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg atgcgggaag
    12361 tccacctttg ctcgcgccat cattggcttg gttaaagcga ccgacggtca tgtggcctgg
    12421 ttaggtaaag agttgctggg catgaagccc gatgaatggc gtgccgtacg cagtgatatt
    12481 cagatgattt tccaggatcc gttggcatca ctgaacccgc gtatgaccat cggcgagatc
    12541 atcgctgaac cactgcgtac ttatcatccg aaaatgtcac gccaggaagt tcgtgagcgc
    12601 gtgaaggcga tgatgctgaa agtcggtttg ttgcctaatc tgattaaccg ctatccgcat
    12661 gagttctccg gtgggcagtg ccagcgtatc gggattgctc gtgctcttat tcttgaaccg
    12721 aagctgatta tctgtgatga gccggtgtcg gcgctggacg tgtcaattca ggcgcaggtg
    12781 gtcaacctgc tccagcagtt gcaacgtgag atgggattgt cattaatttt tatcgctcac
    12841 gacctggccg tggtaaaaca catttccgat cgtgtgctgg tgatgtatct cggccatgcg
    12901 gtagaactgg ggacctatga tgaggtctac cacaatccgc tacatcctta caccagggca
    12961 ttgatgtcgg cagtccccat acctgatccg gatctggaga agaacaaaac catccagtta
    13021 ctggaagggg aattaccgtc gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt
    13081 tgtccgattg ccggtccgga gtgcgccaaa acacgtcctg tgctggaagg gagtttcaga
    13141 cacgccgttt cttgcctgaa agtcgatccg ctttaaaagc aataagggct gacagttgtc
    13201 agcccttatt cacgccaaaa gcgattattt attcccgcca gatgatatgg caaagtttgt
    13261 gatctttttc gcggcataac agaatgcgcg cgaaaacatc gttgatttca ccatcttcac
    13321 tgtccgccag accaatcacc acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat
    13381 gttcctgcca gtcttccgcc ggatcgaata actccgcgcc gccgcgctct tcaaactgaa
    13441 gattgaacag cagaacacca gctggatcga gattgtcggc agccagttcg agaaaaatat
    13501 cgtaggcctg ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc atatccatag
    13561 tcactacctg tttaacctct gttggcgacg ttttacagca acgggctgaa gaagtaaaac
    13621 agtcgctcgg cgacacgttg ccataatgga cgttttagcc ataaacgggc atcgagcaga
    13681 cgagaacgcg aaatataatc gtcctgaacg gcggcgaggt ctgcaccaaa acctttatcg
    13741 tcgattgcca gggtaatctc gaaatttagc cacagactac gcatatcaag gttaacggtg
    13801 ccaaccagac ttagttcacc atcgaccagc acgctcttgg tatgcagtaa cccgccttca
    13861 aactgataaa ttttaacccc agcagccagc agttctgtaa agaatgcgcg actggcccag
    13921 ccgaccagca tcgagtcatt ttttcgcgga aggataatac tgacatccac cccgcgctgc
    13981 gccgccgtgc aaatcgcatg aagtaaatca tcgcttggca caaaataggg cgttgtcatg
    14041 atcaaatatt cacgagccga ataagcagca gtcaataatg cctggtgaat gagatcttcc
    14101 ggaaagccgg ggccagaagc aattgtgtga atagtgtgac cgctggcctg ctcaaacggc
    14161 ataatattga catctggtgg cggcggcaga atacgtttcc cggtttcaat ctcccagtcg
    14221 caggaataaa taatccccat cgcggtggcg atggggcctt ccatacgcgc catcagatca
    14281 atccattgcc ctacgcccgc atcttgtttg aagtagcgag ggtcgaccat attcatgctg
    14341 ccggtgtacg cgatgtaatt atcgatcatg atcatcttgc gatgttggcg caggtccata
    14401 cggcgtaaaa acacacgcat cagattgacc tttaaggctt cgacaacttc aataccggca
    14461 ttacgcatta gctcgggcca cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc
    14521 atcaatcggc aatgaatacc gcgccgtgca gcggccatta atgattcagc cacctggtcc
    14581 gccatgccgc cgggctgcca gatataaaac accatctcaa tattatggcg cgcgagctgg
    14641 atgtcgcgga ttaacgcctg catcacatca tctgactcgg tcatcagttg tagctgattc
    14701 cctttgaccc cagcgatccc ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca
    14761 ctgctatttt cttcggcgaa gatatgctta caggctttaa ggtcgttaag ccattttgcg
    14821 gtggaaggcc acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca
    14881 acggcaagat aggcaataat tccgactaac ggcagaatgt aaataatcaa cagccaggcc
    14941 atcgcggagg gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc
    15001 aaccagtatc ccagaatggc caaccaactc accaacgtat aaacggttgt catagattag
    15061 aaatcctttt gaaagcgtat tgttatgagt ttacgcatat ggattcatct ggcaaataaa
    15121 aacgcgggaa aagcactggt cagcgtcagg ggcgggggta taatgaccat tctgttgttg
    15181 catagagtag ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg catgcagcgt
    15241 caggctagcc gacgtaaatc gcgttggctt gaggggcagt cgcgccgaaa tatgcgtatc
    15301 cacagcatca ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt tgcgatctac
    15361 aatatctaaa tgtactgggc accattgcgg tgcccaataa gtttactctt ccgtctttaa
    15421 actctatttt tgcgccgatt ctttactgtc agctttcgtt tcttgttcat cgttatcgct
    15481 gaaaatgcca tgaccggagt tcaacaacat tgaaacgagc atatcattat taatctcttc
    15541 accatttaaa actcgcgcca gaatttcgct gccaaacagt tgtggtgaga ggatgatatc
    15601 cggatgtacc atcttaatct tattcaggtt tttgctatca ctgacggcga gaactgtttt
    15661 gacatcactg ctcatatctt ttgccgagag tacaacaaac gcgttatctg catcgttatc
    15721 actcagtgcc agaatggccc ggcaacgatc gattcccgct ttctttaaca ctgaactgtc
    15781 attactgtca ccggggataa catcggcgtt atcgcctaag cgttgctcaa gttgcttgat
    15841 atcatcttca ggcaagttgc tgataaccgt tacgttttgt ccgcgttgat tcagttgcag
    15901 aatcgtattg atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat
    15961 tgtatgattg tttcctttta caagtttgtt gaatcccccg cggataagcg gaccaaaaat
    16021 tgaggtcata gatgtggcaa aaacggtaat gccggaaata atgaccgaaa tagtgaacaa
    16081 tcgtgctgat tcagaaacag ggacaatatc gccatagccg acggttgaca tggtttctat
    16141 cgaaaaatag aacgcggtca tcaaactttc tattcgcgga ttaaaacctt cgcttaaata
    16201 aagcgcaccg taagtggagt aaaacagtaa cgtcgtgaaa ctaataaatg cgaaaattgt
    16261 cccggctgct gcgctactgt gggagaagtc tttgcgcagt atcagcaaaa acaccagcgt
    16321 aaaaatgcaa aatccaatac taaatttcag ccagggataa aaatgcagag tgtaaattag
    16381 cgctatcaac aacaaaatga tactgattgc ccatgcgagc tttgctcgga acagtaagcc
    16441 aatggagttc aggacaagaa atacgccgag cataaatagt ggggcgtttg ccagcaagga
    16501 gagatccagc tctgaaaatg ctttgatatg gaaaatatca agcaaattga ccgacatacc
    16561 atagattgtt ttaaaaataa gcaatccatt taaaaagacg gccaacgcga gaatgtcgtg
    16621 ccgtagggtc acccataact ttgttgctgt ttgtttgaat gtagtccagt gactcactga
    16681 tacctctgta accacaattt tgtttaatta tctcaacgtg cacattttct gaacatacat
    16741 gcggcgcgtg aatgtgttaa acaatagtag atgactttat ccgcagtaat caaaggtttt
    16801 gagtgagcaa ctctgcgtca ggaaaaggca ttttcctgaa tatgttcaca tcatataaag
    16861 aaatacagat aaagttatta tctgcttgtg gtggtgaatg cactgaccga cctataagga
    16921 aaggccaaac aagaacacgg ttgcaaaaac cgtgccctta aatattgaat ctctattcag
    16981 aacactttct taaatggttt cactgaaacg tgttcataga ctcctgccgc tacgtacggg
    17041 tcagcatcgg cccaagcctg agctgcttcc agcgactcaa attcagcaat aacggttgag
    17101 ccagtaaatc ccgcagcccc tggatcgtta ctgtctaccg ctggcattgg accggcggtc
    17161 aacaaacgac cttcatcatg cagtaactgt aaacgtgcta aatgtgccgg acgaacggaa
    17221 aggcgttttt cgaggctatc agctttgtct tgagcgtaaa taacatacaa cacaggcaca
    17281 actccttgtt cgggaaagtt gtaaagtacg ttatttgaaa gggcgaagat ctgcaacgga
    17341 aagatgatgt ctttgttaag accatgcata aagtaaggtc aattacgcca aaaatgacat
    17401 tttcactgat cctgatcgtc ttgccttatt gaatatgatt gctatttgca tttaaaatcg
    17461 gggcctggtt tttctactga aatgattatg acttcaatga cccttgattt acctcgccgc
    17521 ttcccctggc cgacgttact ttcggtctgc attcatggtg ctgttgtggc gggtttgctc
    17581 tatacctcgg tacatcaggt tattgaacta cctgcgcctg cgcagccgat ttctgtcacg
    17641 atggttgcgc cagcggatct cgaacctcca caagccgttc agccaccacc ggagccggtg
    17701 gtagagccag aaccggaacc tgagccgatc cctgaaccgc caaaagaagc tccggtggtc
    17761 attgaaaagc cgaagccgaa acctaagcca aaaccgaagc cggtgaaaaa ggtggagcag
    17821 ccaaaacgcg atgtcaaacc cgtagagtcg cgtccggcat caccgtttga aaatacggca
    17881 cccgcgcgcc cgacatcaag tacggcaacg gctgcaacca gcaagccggt taccagtgtg
    17941 gcttcaggac cacgcgcatt aagccgtaat cagccgcagt atccggcacg agcacaggca
    18001 ttgcgcattg aagggcaggt taaagttaaa tttgatgtca cgccagatgg tcgcgtggat
    18061 aacgtacaaa tcctctcagc caagcctgcg aacatgtttg agcgtgaggt gaaaaatgcg
    18121 atgcgcagat ggcgttatga gccgggtaag ccaggcagtg ggattgtggt gaatatcctg
    18181 tttaaaatta acggcactac cgaaattcag taagcataaa gtcaaaagcc tccgaccgga
    18241 ggcttttgat tattactcaa caggtaaggc gcgaggtttt ccttcgggat caaccgcgac
    18301 atacttaaat aatgcttctg ttgctttata gcgttgcccg attggttcag acgccacttt
    18361 tttcacccac acttcaatat taatgctgac tgatgtcgtc cctttctgga cacagcgtgc
    18421 atagcagcac accacatcgc cgaccgcaac cggccgtaag aaagtcattc cttcaacccg
    18481 cacggtcact acacgaccgt gggcaatttc tttcgccaga atagcgccgc caatatccat
    18541 ttgtgacatt aaccaaccac caaagatgtc accattggca ttggtatcgg cgggcatggc
    18601 taaagtacgt aaaacaagat cgccctgagg gacgttatgt gttgtagaca tggtaaaacc
    18661 gacttaaaag gaaaatcaca ggcgcgatgc tactatgatt ttcctgagga gaacagagga
    18721 gatacagcgc cagccccgag ggactggcag tctggttagg atttatcttc ctgcggcatg
    18781 tggcggtaga tatagatacc gcttaacaat gtgaagatta aggtaagggc ggtcaggcca
    18841 aagactttaa agttgaccca aatattttgc ggcagccaga atgcgatgta gatgtttgcc
    18901 aggccgcaaa ggataaagaa aacagcccag gccagattca gcttcgacca taccgattgc
    18961 ggcagggtga gttctttacc cagcatccgc tgaattagcg gctttttcat cacccattgg
    19021 ctgactaaca gggcacccgc aaacagggca taaatgacag taaccttcca tttaataaac
    19081 tcatcattgt ggaagaacag cgttaagcca ccgaagacga ccaccagaac aaaagtgatc
    19141 agggccatct tctcaacctt acgaaagcga acccagctat atataagcac aatcgccgtg
    19201 gcgacgatga gcgccgcagt agccgcatag atgtcataaa tcttgtaaaa cgcgaaaaag
    19261 acaaccagcg gtaaaaaatc aagaaactgc ttcattttac gattccgtaa tcaaatgctt
    19321 aaggttattg gcgaatcaac atatacaggc ggaacagata gatgagcaat acggctgaaa
    19381 tcaggttgct caaggtgttc gccagtacgg caccaatttc cggggttaat gcggcaaaag
    19441 aagaggcaaa aagcagcaac agtgtttttg ccagcaacca gctcagtact gcgggtgcca
    19501 ccagacgcat attcgcccat gtcagccgca tactgctacg catcgaggca aaaacgccca
    19561 ttttgtcctg taccagcatc accggtgcca gagccagtaa aatggccatg ataattcccg
    19621 gaacgaccac cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa atcagaataa
    19681 ataactttgg caatatcggc gcactggcac caatagctcg cagcgcactg actctctgac
    19741 ccgcagacac cagctggata attaatatta cgcccccggc gagaatggcg ttaccgatta
    19801 atcctgaaaa agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt tccggtgaca
    19861 tattctgaac caggtcgaac aacccactac tgccgctaac gggtacgccg tcattgagct
    19921 gcgcaagctg tgcatcactg ggtgagaaaa catgccctaa caccactgtg ataaacgcac
    19981 atagcaacga taccaacaga atggtcataa attgattacg gaaaaaattt ccggtgtcac
    20041 ggtatacgga ctgcgccgtg atagacatgc actctccttg agttttatgc aggtgttaat
    20101 tagcgggcaa ttgtaccctg gttaagcccc gggtggcagc atcaggtctt gtatggaaaa
    20161 gcatatcttt gtaaagcgga ggtaattgct ggccttcagg cgtgggtaaa ccgcttcaag
    20221 tggcgagcga gttaatattt gcgtagcgaa aatatttaaa aattgattta aatcacatta
    20281 accaggattt tcaatgcaac ttctaaatta atccagatca ataaatggtg aattaccata
    20341 tgtaatgtga tctgtgtagg atcatttgtt accccaatgt gggtatattc gtcacgtttt
    20401 tataaccata acgacggagc ggatatgaaa aagttaacag tggcggcttt ggcagtagca
    20461 actcttctct ctggcagtgc ctttgcgcat gaagccggcg aattttttat gcgtgcaggt
    20521 tctgcaaccg tacgtccaac agaaggtgct ggtggtacat taggaagtct gggtggattc
    20581 agcgtgacca ataacactca actgggcctg acgtttactt atatggcgac cgacaacatt
    20641 ggtgtggaat tactggcagc atcgccgttt cgtcataaag ttggcacagc cactaccggg
    20701 gatattgcaa ctgtccacca tttaccgcca acactgatgg cacaatggta ttttggcgat
    20761 tcaggcagta aattccgtcc ttacattggt gcgggtatta actacaccac cttttttaat
    20821 aatgacttca atgacaccgg tgaaggtctt ggtttatctg accttagtct gaaggattca
    20881 tggggcgtgg cggggcaagt aggtctggat tatctcatca accgtgactg gttgctgaat
    20941 atgtcggtct ggtacatgga tatcgatacc gaagtgaaat ataaatccaa cggcgcagtg
    21001 ctgcctgcag ggcgttattc tgacaacgta cgccttgacc cgtgggtatt tatgttctct
    21061 gcgggttacc gcttctaatt ttttaccatc aataaaccgc cagatgtttt ctggcggttt
    21121 gtctcaaatt aactgcgcgt cgccgctttc atcggttgta caaaagcttt cagtgccgcc
    21181 agcattttct ctggctcatt aatatgttgc tcgatgattt taacgatggc cgaaccagaa
    21241 atcgcgcccg cagctcctgc atcaatcgct gcttttacct gatccggggc ggaaatacca
    21301 aatccctgca atggaggcgc agcgttgtac tctttcagct tcgtgaccag atgattgagg
    21361 ggtaacgcgg cgcggttttc tgcgccggtc acgcccgcac gtgacaataa ataggtgtat
    21421 ccacgaccat aggaggctat ctggcgcagc aaatcatcgg cggcattcgg cgggcaaata
    21481 aaaataggtg cgacattatg acgcaacgcg gcctggcgga agggcgcgga ctcttccacg
    21541 ggcacatcgg caaccagcac cgaatcgacg ccgactttct cgcactcggc ataaaactca
    21601 tcaatgcctt tgttaaacac caggttggca tacatcaaaa ggccgatggg aatggtcggg
    21661 tgcttctggc gaatgagtgc cagcatctca aagcactgcg ccggggttac tcccgccgca
    21721 aaagcacgca gtgtggcgtt ttgaatcgtc gggccatccg ccagtgggtc ggagaagggg
    21781 atgcctaact ccagcgcgtc agcaccggct tcaattagcg tatcgataat tttcaacgac
    21841 tgctcaatgc ccggatcacc gagggtgacg aaaggaacga atgcgccttc tttgcgctcc
    21901 ttcaactggg caaacagaga ttcgtagcgt tccatcagat ttcccctcgt gctttcaaaa
    21961 tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga aaggttaacc accagtagct
    22021 gctctttttc cggattttcg cgcatcattt tcagcgcatg ggccagggcg tgggaggatt
    22081 ccagcgccgg gatgatccct tcatgcaggc aaagcgtttt aaaggcttcc agggcttcat
    22141 cgtcggtaat agacacgtaa tcagcgcgtc cagtgctgtt gagatacgca tgttgcgggc
    22201 cgacggacgg gaaatccagc ccggcagaaa tggagtaaga ctcttcaatt tgcccgtctt
    22261 cggtttgcat catcggcgct ttcataccga aatagatgcc cacgcgacca tgttttaacg
    22321 gtgcgccgtg ctcgccagtt tcgataccgt ggccgccagg ctccacacca atcaggccga
    22381 cgtcggtttc gttgatgaaa tctgcaaaca taccgatggc attcgaacca ccgccaacac
    22441 aggcgataac ggcatccggc aggcgacctt ctctttccag aatctgcgct ttcgtttctt
    22501 cgccaatcat ccgctgaaac tcacgcacaa tggtcgggta aggatgcggg ccagctgcgg
    22561 tacccagcat atagtgcgcg gtttcataac tgccggacca gtcgcgtagc gcctcattac
    22621 aggcatcttt cagggtcgcg gaaccgctat gtaccgggat cacttccgca cccattaagc
    22681 gcatccggaa aacgttaggc gactggcgtt caacgtcttt ggcacccata taaatacggc
    22741 atttcaggtc gagcagggcg ctggcaaggg ccgacgccac gccatgctga ccggcaccgg
    22801 tttcggcaat aatttcagtt ttacccatcc gcttcgccag taaagcctga ccgagtacct
    22861 ggttagtttt atgcgcgccg ccgtgcagca aatcttcgcg cttcagatac agcgtggtgt
    22921 tcgtcccggc tgtaatgttc tggcatttgg tcagcgcggt tggacgcccg gcatagtttt
    22981 tcagcaggtc gttgaactga gcctgaaatt caggatcttt ttgcgcgctg acaaaagctt
    23041 cttccagctg gcgcagagca ggcatcagga tttgtggcac gtacatgccg ccaaactcac
    23101 caaaataggg gttaagtaat gttgtcattg ttcctttcct taatatgcgc gcagcgtctg
    23161 gaaaaccgag gccaaaagac gtgcgtcttt gatacccggt tgcgactcta cagcagaatt
    23221 aaaatcaagc ccggcgcagc cggtttgtgc cgcttccacg cagttatctg cgcctaagcc
    23281 ccccgccagc agaacgttgc caagcgattg accatttaat agtgaccagt cgaaacgttg
    23341 tccgctcccg ccctgaccgt tgtcgaatac atatttatcg atgtgctgaa aatcgcgcgc
    23401 gggaagagtt tcaccgacac ttaaagcctt ccagatggcg acgtgtgctg gcagagcctc
    23461 acgcagagtg tcgatataca gctgatcttc attaccatgc agttgcactg ccgccagcga
    23521 taacacctta gctttgtcca ccacatcggc aatatcgtga ttgcggaaca cgccaacata
    23581 ctgcaacggt gccgcagcca tcacttcctg cgcctgttca acgttgacgc aacgcggtga
    23641 tgtcgcaaca aaaatcagcc caccgtaaat cgcgcccgcg tcataagctg ctttagcatc
    23701 ttgttcacgc gtcaggccac atactttatt ctcacccagc aacacccgac gcacggcggc
    23761 gttcaaatcg tcatgggcca tcaacgccga accaatcaga aagccgttag tgaagtggct
    23821 taactcgcgc acctgagcgt aagtattgat gccggattcg ctgattaccg tcacgttgtg
    23881 ccccagtttc ggcgcaagct cgcgggtacg gttgagatca atcgacaaat cgcgcagatc
    23941 gcggttgttg atgccaacga cctttgctcc caatgcaatg gcgcgctcca gttcctcttc
    24001 attactgact tcggtcagca cacccatctc cagactgtga gcgacggtgg caagctggcg
    24061 atattgttcg tcatccagta ctgaaagcat taataagcag gcatcggcct ggtaatagcg
    24121 cgccagatag atctggtaag gatcgataat gaagtcttta cataaaatcg gctgcggggc
    24181 gatttggctg acgatgggga ggaaatcaaa gctcccctga aaatatttct catcagtcag
    24241 cactgaaatt gccgaagcgt aatgtttata aatggcggca atgcgtgccg gatcgaaatc
    24301 atcacggatc acgccttttg acggcgaggc tttcttgcac tcaagaataa acgccgtgcg
    24361 tgcaccctgt agcgcatcat aaaaatgtcg cgtgctcggc tgaatctcat tctgaaaact
    24421 ggccagcggt tgctgctgtt tgcgggtttc tacccaaatc gccttgtctg cgacgatttt
    24481 cgctaaaacg gtttgcatca tttaccctcg tgccgccagt gcggtgactc tgtcgtaagc
    24541 ggaaccactg cgcagtacct caagaacggt ttgcgcattg gcttgcagat cttcatggcc
    24601 atgcaggcgc attaacatgg cgacattcgc cgcgacggct gcttcgtgag cggcgtcgcc
    24661 tttaccttgt aacaaacgtg ttaaaatgtc acggttttct tccggtgttc cgcctgccag
    24721 ttgctcctgg tggtagggtg tcaggccaaa gtcttctgcg gtgagctgat agcttttaat
    24781 ttcgccgtca tgcagttcgg caacgattgt cggcgcgtgt aatgaaactt catccatccc
    24841 gccgctgtgc accaccgccg cgcgttgata ccccagcacg cgcaaagttt cggcaatcgg
    24901 cagcaccagt tccggactat aaacaccaat tagcgccagc ggaggatgcg ccgggttaat
    24961 caatggcccc agcacattga acagtgtgcg ggtttttagt cgctggcgaa ccggcattgc
    25021 atggcggaat ccggtgtgat atttcggtgc aaagaggaaa cagacgccta actcatccag
    25081 cgcctggcgc gatttatcgg cgttcatatc aagattaata ccgaacgccg ccagcagatc
    25141 cgacgagccg gatttactgg agacgctacg gttgccgtgt ttcgccactt tcagcccgca
    25201 ggccgcggcg acaaacgcac tggcggtaga aatattgatg ctgttgctgc cgtcaccgcc
    25261 agtaccgacg atatcggcaa acagataatc cgggcgcggg aacggcgcgg cgttttccag
    25321 tagcgcggtt gccgccccgg cgatctcatt cgggtgctca ccgcgaattt tcatgctcac
    25381 cagcgccgcc gccagttgtt ccggcttcag ctcgccacgt accaccgctg aaaacagctg
    25441 gtggctttct tgttggctaa gcgtctgggc ctgatacagt ttttccagaa tcggttgcag
    25501 cgtgttggtt ggctttagtt tctgctgcgc ccaggccagc gtttgttcca gcaggcgagc
    25561 gccctgggta gtaagaatgg attccggatg gaactggaat ccacaaacgc gatccgcatc
    25621 atgacgcacc gccatcacca tgccattaaa atgggcgttg atggttaaac cggccggaat
    25681 attactgcca accagcgagt gataacgcgc cactggcagc gggtttgtta atccggcaaa
    25741 catcgcctga ccgtcatgtt caatgctcga cgctttaccg tgaagaattt cgcccgcctg
    25801 accgacatag cccccgtaag cttcgacaat cgcctgatgt ccgaggcaaa tgccaataat
    25861 tggcagcttg ccacgcaagc gggtgaggag ttccggcata caaccggctt cgctcggcac
    25921 accggggcca ggagaaagca tcagcaccgg attgctcatc gtcgccaggc gttcaattaa
    25981 ggtctgcgcc ggaatatggt tgcggtaaat caccacgtta tgaccattgc tgcgcaactg
    26041 atctgccagg ttgtacgtaa aagagtcgat attatcgagc agcagaatgt cagccattag
    26101 aacgtctcct gtgcatgatg cgcggtggca atagcgcgca gtacagcgcg ggctttatta
    26161 cgagtttcgt cggcttccga ctgcggaaca gaatcaagga ctacgccagc accggcttgc
    26221 acggtggcga taccgttttc caccagcgcc gagcggatca caatgcaggt gtcgagatcg
    26281 ccatgcgcgg taaaataacc taccgcgccg ccgtagctgc cgcgtcgacg accttctgct
    26341 tcggcaatta actgcatagc gcgtactttc ggtgcaccgc ttaacgtccc catattcata
    26401 caggcgcggt aagcgtgcag ggcgtcgaga tcgtggcgca gctcaccaac aacgcgggag
    26461 accaggtgca tcacgtaaga gtaacggtca actttggtga gatcggcgac gtagcggctg
    26521 ccgggtgtgc aaatgcgagc caggtcatta cgggcgagat ccaccagcat cagatgttca
    26581 gaaagctctt tatgatcggt acgcatctcc agttcgatgc ggctgtcgag gtctctgtcc
    26641 agcgaaccat cggcacgacg accgcgtgga cgtgttccgg caatcgggta aatctcaatc
    26701 tggcggctgg tggcgtcata cttgagcgaa ctttccggcg acgcgccaaa cagggtgaaa
    26761 tcattatcct gcataaaaaa catgtacggg ctgggattac tctttttcag cacgtaatag
    26821 gccgccagcg gtgacgggca gggcagagag aaacggcgag atggcaccac ctggaaaatt
    26881 tctccggcgc gaatcgcttt ttgcaacaaa cgcactacgc caccgaactc ttcatcgctc
    26941 tggttacatt cacaacgcat atgcggcacg gaaaccaccg gcagcggcgg cgcggcttcg
    27001 gtcagttgct gacgtagttc gttaaggcga gcagtgagac gttgtttttc ttcttcattc
    27061 ggagcaaaca ggctggcctg aatacgggtg ctttttttct gatggtcaat caccatcagc
    27121 gtttcagcga gataaaaaca gaaatcaggg cagctatttt ccgctgacag ttgcggtaaa
    27181 ttttcaaatc ccgccacaag gtcataagag aacaggccgc cgaagaacat tgcttctcgt
    27241 tcttccttcg gtacattcaa cagattctgt aataagcgga aagcgtcaaa aaccgaaagg
    27301 gagcataagc gggcgtcttc atccagcagt ggactgacag gcgggaagcg cagtacgcgg
    27361 cagtttggtg attgttcatt ttccacaccc gcaggcaagg cgttatccag tagtgtcaac
    27421 agggcttctc cattgccgga aagtgcctgg attgtgacag tgtcacttaa tgctgtaatg
    27481 cgcagcgcac tgtctaccag cagcaggctt tttaaatcat ctttgctgtc gatatctgcg
    27541 gattccagca gcagcgttgc cggacgatcc ccacacaact ggtgaaaaag cgcagtcggg
    27601 ttgtcgcgat aagcgccttt gcaggttagc agttcgagag tcggtttttg tgtttgcatt
    27661 gttattctct aattttgttc aaaaaaaagc ccgctcatta ggcgggctgg gtatctgatt
    27721 gctttacgca tggtgaatac actgcccgtt tcaggaagtg cgccaccaac ctttcagtac
    27781 gaaaattgct ttcattgtcg ataccctttt tacgtgaact tgcgtactag ttaactagtt
    27841 cgatgattaa ttgtcaacag cttatttcag aatatttgcc agaaccgtta tgatgtcggc
    27901 gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga cacgaattac gcagtgattt
    27961 acgacctgca cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc
    28021 accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg
    28081 caattgcacc agcaagagag gaaatttcac gttcgggact ggcgctgaat cttattcctg
    28141 gtgtggaaat ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg
    28201 atattactca tccgctaatg tgcgagttcc tcgcgcagca gacagaacgg cgcaatcagc
    28261 gggcgcagct gattgccgaa agacttgaaa aagcgcaaat acctggggca ctggaaggcg
    28321 cgcaacgact ggcgcagggc ggtgcggtga cgcgcggtca ttttgcgcgt ttccttgtgg
    28381 agtgcggaaa agccagttca atggcggatg tctttaaaaa gtatctggcg cgcgggaaaa
    28441 ccggatacgt tccgccacag tggtgtacaa tagagcaatc tattgatgtc attcatcatt
    28501 ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga
    28561 aaagattggt tgcgcatttt gccgaacacc acggcgacgc gatggaagtc gcgcagtgcc
    28621 agcaatcgcc caatgaacgc acccagctgg cgacacttgc gcgtcagcat catttatggg
    28681 catcacaagg atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct
    28741 ggctgcccgc aggcgttgaa ggcgtctggc agctatggga acagccgcag aacaccacag
    28801 agagggaatt atgagccagt ttttttatat tcatcctgat aacccgcagc aacgcctgat
    28861 caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt gtttatccaa ctgattccgg
    28921 ctatgcgctc ggctgtaaaa ttgaagataa aaacgcgatg gagcgtattt gtcgtattcg
    28981 ccagctgccg gacggtcaca actttaccct gatgtgtcgc gatctttctg aactctcgac
    29041 ctattcattt gtcgataacg ttgcgtttcg tttgatgaaa aacaacacgc caggcaacta
    29101 taccttcatc ctgaagggga cgaaggaggt gccacgccgt ctgttgcagg aaaaacgcaa
    29161 aaccatcggt atgcgcgtgc cgtctaaccc tattgcgcag gcgctgcttg aagcattggg
    29221 cgaaccgatg ctttccactt cgctaatgct gccaggcagt gaatttaccg aatccgatcc
    29281 ggaagaaatt aaagaccgtc tggaaaaaca ggtggatttg attattcacg gcggctatct
    29341 cgggcagaaa ccaacaacgg ttattgatct caccgatgat acgccggtcg tggtgcgtga
    29401 aggcgtaggt gatgtgaagc ctttcttata acaaattgcg ttccacggat ggaagatgat
    29461 ggcagggata tatcgctgca ttttgctgtt gatagttggg ctttttttct cttcgttgag
    29521 ttatgcgaaa aacacggaga tcccttctta tgaagaaggg atctcgctct ttgatgttga
    29581 agccactctg caaccgaatg gggtgctcga catcaaagag aacattcatt ttcaggcgcg
    29641 aaatcagcag attaagcacg gattttatcg tgatttaccg cgactctgga tgcagcctga
    29701 tggggacgct gcactgctga actatcatat tgttggcgtc acccgtgatg gtattcctga
    29761 accctggcat cttgactggc atatcgggtt aatgagtatt gtcgtgggcg ataaacaacg
    29821 tttcttgcct caaggcgact atcattatca aattcattat caggttaaaa atgctttcct
    29881 gcgtgagggg gattctgatc tgctaatctg gaacgtgacc ggtaaccact ggccgtttga
    29941 aatttataag accctatttt cactcaagtt gcctgatatt gcgggtaatc catttagcga
    30001 aatcgatctc tttactggag aagagggcga cacatatcga aatggccaca tccttgagga
    30061 cggaagaatt gaatcccgcg atccgtttta tcgtgaagat ttcacggtcc tctaccgctg
    30121 gcctcacgca ttacttggta atgccccggc tccacaaacg acgaatattt tcagtcatct
    30181 tcttttaccc tccacgtcaa cgttgttaat ttgctttccg tgcctgttcc tggcgtgtgg
    30241 atggttatat ctctggaagc gcaggccgca atttacgccg gtagatgtaa ttgaaaccga
    30301 tgtcattccg ccagattaca cgcccggcgt gttacgtctc gatgcgaagc tggtttacga
    30361 cgataaaggt ttttgtgccg atatcgtaaa tctgattgtg aaaggaaaaa ttcatctgga
    30421 agatcagtat gataagaacc agcaaatcct gattcgtgtt aatgaaggcg cgaccagaaa
    30481 taatgcggta ttactgcccg cagagcagtt attactggaa gcgttatttc gtaaaggcga
    30541 taaggtcgtt cttacgggga gacgcaacag agtcttacgc agggcatttt tacggatgca
    30601 gaaattttat ctgccgcgta aaaagtcttc gttttaccgg cctgatacgt ttttgcaatg
    30661 gggcggaatg gcgatattgg cggtcattct ctacggtaac ctgagtcccg taggttgggc
    30721 aggaatgagt ctggttggcg atatgtttat tatgatctgc tggcttcttc cttttttatt
    30781 ttgttccctt gagcttttgt ttgcccgcga tgatgacaag ccttgcgtta atcgtgtaat
    30841 catcactttg tttttaccgc tgatttgttc aggcgtggcc ttttattctc tctatatcaa
    30901 tgtcggagat gtattctttt actggtatat gccagcgggt tattttagcg ctgttttcct
    30961 gaccggttat ctcactggca tggggtatat ttttctgcca aagtttaccc aaactgggca
    31021 gcaacgttat gcccacggtg aagctatcgt taactatctt gcgcgtaaag aggcagcaac
    31081 acacagtggg cgtcggcgga aaggggaaac acggaaactg gattacgcgt tgttaggttg
    31141 ggctatatcg gcaaatttgg ggagggaatg ggcgttacgc atcgcccctt cgctttcttc
    31201 ggcaatttgc gctccagaga ttgcccgtaa cggcgtttta ttctcattac agacgcacct
    31261 gagttgcggg gcctatacca gtttgttggg gcggagctat tctggtggtg gttctggcgc
    31321 tggtggcgga ggcggtggtg gttggtaacc aaatcgacgt taatcgaaac aagctttaac
    31381 cataaagata gtgtagtatg ttgcgcctca aagccaggcc agttaaaacg tcgagtcgtt
    31441 tacttaaggc ctgaagagtt caaacagact ttactatcag tgagtcagat gtaagttctg
    31501 actcattgtt gcacaaaagc atatcagacg cctgggaagg cgacaccaaa aggaagctct
    31561 atgagcgaaa agctacagaa agtgctggcg cgtgccggcc acggttctcg ccgtgaaatc
    31621 gaatctatta ttgaagccgg tcgtgtgagt gttgatggca aaattgctaa actcggcgat
    31681 cgcgttgaaa ttacccctgg actgaaaatc cgtatcgatg gtcatctgat ttcggtacgt
    31741 gagtccgctg aacaaatttg tcgcgttctg gcctattaca aaccggaagg tgagttgtgt
    31801 acccgtaacg atccggaagg gcgcccaacg gtgtttgacc gtctgccaaa actgcgtggc
    31861 gcacgctgga ttgccgtagg tcgtctggac gttaacacct gtggtctgtt gctgttcacc
    31921 accgatggtg aactggcaaa ccgtttaatg cacccaagcc gtgaagttga acgtgaatat
    31981 gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc gtgatttgag tcgtggcgtg
    32041 cagttggaag atggtccggc agcctttaaa accatcaagt tcagcggcgg cgaagggatc
    32101 aaccagtggt acaacgttac tctgaccgaa ggccgtaacc gtgaagttcg tcgtctttgg
    32161 gaagcggttg gtgtgcaggt gagccgcctg attcgtgttc gttacggtga tatcccactg
    32221 ccaaaaggtc tgccacgcgg tggctggacg gagctggacc tcgcccagac taactatctg
    32281 cgcgaactgg tggaactacc gccagaaacc agctctaaag tcgctgtaga aaaagaccgt
    32341 cgtcgtatga aggcgaatca gattcgtcgt gcggtgaaac gtcacagtca ggtgagcggc
    32401 ggtcgtcgtt ctggcggacg taataataac ggttaatcaa agtatgccgg acgtcatatc
    32461 cggcattttt acagattagt aatcaatccc tatctgcgct tttacaccgg catcaaacgc
    32521 atgtttcacg gggcgtaatt cactcaccgt atccgccagt tcaagaatat cccgatgaca
    32581 gcctcgaccc gtgataatca ccgtctgttg atgtggacgt tcatttaacg cctgcaccac
    32641 ttcttccagt ggcaaatagt catacgccac catatacgtc agttcatcga gcaaaaccat
    32701 atccagtgaa ggatcggcca gcatccgctt tgcatgttgc cagacttcgc ggcaggcggc
    32761 ggtatcagac tctcggtttt gtgtatccca ggtaaagccc gttgccatta cctgaaactc
    32821 aacgccgtgt ggctccagca gatttcgttc accattaggc caggtgcctt taataaactg
    32881 cacgacgccc actttttttc cgtgaccaac tgcgcgtgtt gccgtaccaa atgccgcggt
    32941 ggttttgcct tttccattgc cggtaaagac gatgataata ccgcgttcat cctgggcctg
    33001 ggccacacga gcatctactt tttctttcac tcgctgctga cgctgttggt agcgttcatc
    33061 actcattggg aaattcctgg tttacggccc ggttgggcgt caaaggtcat gccggtttta
    33121 cggcggctgt catcgcccat cagccagagg tagagcggca tgatatcagc gggggtttta
    33181 agtttctgtg gatcttcggt cgggaaggcg ctggcccgca ttgcggtgcg cgtaccgcct
    33241 gggttaatgc agttgacacg caggcgctgc tgatattcat cggccagtac ctgcatcatc
    33301 ccttcggtgg caaatttcga cgctgcatat gcgccccagt ttgcacgccc ctgacgtcca
    33361 acgcttgatg aggtaaagac cagtgaaccg gcatccgatt tgagtaataa aggaagcagt
    33421 gcctgtgtga gcataaaggt ggcattaacg ttgacctgca tgacgtcctg ccagacctgc
    33481 ggatcttgtt cgctcattgg gcaaacatcg ccgagcaatc cggcattatg caaaacacca
    33541 tccagacgcg gataattaac ggcaatgcgc tgtgccagtt gttggcaatc ttcggacgtg
    33601 caggttagca aatcgagaat aaaccactgt ggctgacgtc caatttcttc gtttatgtgg
    33661 ctggctacct gacgtaattt ttcttcatta cggcccaaaa gaatcactgt cgcaccatag
    33721 cgtgcatacg tcatcgctgc ttcacgacca attccatcgc tggctcccgt caccaggata
    33781 atgcgatcat tgagtaaatc ttgttttggc tggtaatgca tggctactcc tcaacgacgt
    33841 tgtctgtcgc gctgccagtg tactttatga ctttggggct ttatgcctga aacagaaggg
    33901 catttcaatc agcctggggc gataacaccg caaaatggat actttgtcat actttcgctg
    33961 caataacatc tctgcgagac ggctaaacat gcctgttgta aactgtgagc caaagcgttg
    34021 tttaaccaag gtggggactc gtggaattgt tgtctgaata tggtttgttt ttggcgaaaa
    34081 tcgttaccgt tgtgctagcg attgcggcga ttgccgccat tattgtcaat gttgctcaac
    34141 gtaataaacg ccagcgtggc gagttacgag tcaacaatct cagcgaacag tataaggaga
    34201 tgaaagaaga actggccgcg gcgctgatgg acactcatca gcaaaaacag tggcacaaag
    34261 cgcagaagaa aaagcacaag caagaagcga aagccgcaaa agcgaaagct aaactgggcg
    34321 aggtggtaac tgacagtaaa ccccgcgtct gggtgctgga ttttaaaggc agcatggacg
    34381 cccatgaagt gaactcgcta cgtgaagaga taacggcggt actcgcagca ttcaaaccgc
    34441 aggatcaggt tgtgctccgt ctggaaagcc caggtggcat ggtgcatggt tacgggttgg
    34501 cggcttcgca gttgcagcgt ctgcgtgata aaaacattcc tttaactgtt acggtagaca
    34561 aagtcgctgc cagcggcggt tacatgatgg cctgtgtggc ggacaaaatt gtttccgcac
    34621 cgtttgctat tgtgggttcc attggggtgg tggcgcaaat gcccaacttt aaccgcttcc
    34681 tgaaaagcaa agatattgat atcgaactgc acaccgccgg gcagtataag cgcacgctga
    34741 cgttgctggg tgaaaatacc gaagaagggc gggagaaatt ccgcgaagag ttgaacgaaa
    34801 cgcatcagtt atttaaagat tttgtgaagc gtatgcgtcc gtctctggat attgaacagg
    34861 tggcaacggg tgaacactgg tacggacaac aggcagtaga gaaaggcctg gttgatgaaa
    34921 tcaacaccag tgatgaagtt attcttagcc tgatggaagg ccgggaagtg gtcaatgtac
    34981 gctatatgca gcgtaaaaga ctcattgacc gattcaccgg cagcgcggca gagagcgccg
    35041 atcgattgtt gttacgctgg tggcagcgtg ggcaaaagcc gttgatgtaa aagacaaacg
    35101 cgaggataag acctcgcgtt ttgctttaat caaccaggtg atatttttct gaaagcacat
    35161 gggccaggtg tttgaacata ttaaacaccg cggtgctttt ggcagttggt aatccttgtt
    35221 catctaaaaa gtagtcgccg gtaaatacca gcacgccatt acgctgcgtg acgccggtgg
    35281 cttcaatccc tgcgagcgta tcctcatgct cacgaatgat tttgttggcc tctttcaaca
    35341 gcgtttcgcg gtcgatgggt tgagtctctt tgttcattat ttactcctta aacaaggaca
    35401 ttagtctacg ccaggcatgg cttgcagaca aatataccac gctggtggca agagcgcctt
    35461 actggcaact ttggattttg catgctaata aagttgcgta tcggatttta tcaggtacag
    35521 tgtgacgctt tcgtcaatct ggcaatagat ttgcttgaca ttcgaccaaa attccgtcgt
    35581 gctatagcgc ctgtaggcca agacctgtta actcagtcac ctgaattttc gtgagcagag
    35641 tcacgacaag gggttgatat ccgcagagag cgagtccata tcggtaactc gttgccagtg
    35701 gaaggtttat caacgtgcga cgcattcctg gaagaatcaa attaggtaag gtgaatatgg
    35761 gtaaagctct tgtcatcgtt gagtccccgg caaaagccaa aacgatcaac aagtatctgg
    35821 gtagtgacta cgtggtgaaa tccagcgtcg gtcacatccg cgatttgccg accagtggct
    35881 cagctgccaa aaagagtgcc gactctacct ccaccaagac ggctaaaaag cctaaaaagg
    35941 atgaacgtgg cgctctcgtc aaccgtatgg gggttgaccc gtggcacaat tgggaggcgc
    36001 actatgaagt gttgcctggt aaagagaagg tcgtctctga actgaaacaa ctggctgaaa
    36061 aagccgacca catctatctc gcaaccgacc ttgaccgcga aggggaagcc attgcatggc
    36121 acctgcggga agtgattggt ggtgatgatg cgcgctatag ccgagtggtg tttaacgaaa
    36181 ttactaaaaa cgcgatccgc caggcattta acaaaccggg tgaactgaat attgatcgtg
    36241 ttaatgccca gcaggcgcgt cgctttatgg accgcgtggt ggggtatatg gtttcgccgc
    36301 ttctatggaa aaagatcgct cgtggtctgt ctgccggtcg tgtgcagtcg gtggcggtcc
    36361 gcctggtggt cgagcgtgag cgtgaaatta aagcgttcgt accggaagag ttctgggaag
    36421 tcgatgccag cacgaccacg ccatctggtg aagcgttggc gttgcaggtg actcatcaga
    36481 acgacaaacc gttccgtccg gtcaacaaag aacaaactca ggctgcggta agtctgctgg
    36541 aaaaagcgcg ctacagcgtg ctggaacgtg aagacaaacc gacaaccagt aaacctggcg
    36601 ctccttttat tacctctacg ctgcaacaag ctgccagcac ccgtcttggt tttggcgtga
    36661 aaaaaaccat gatgatggcg cagcgtttgt atgaagcagg ctatatcact tacatgcgta
    36721 ccgactctac caacctgagt caggacgcgg taaatatggt tcgcggttat atcagcgata
    36781 attttggtaa gaaatatctg ccggaaagtc cgaatcagta cgccagcaaa gaaaactcac
    36841 aggaagcgca cgaagcgatt cgtccttctg acgtcaatgt gatggcggaa tcgttgaagg
    36901 atatggaagc agatgcgcag aaactgtacc agttaatctg gcgtcagttc gtagcctgcc
    36961 agatgacccc agcgaaatat gactccacga cgctgaccgt tggtgcgggc gatttccgcc
    37021 tgaaagcacg cggtcgtatt ttgcgctttg atggctggac gaaagtgatg cctgcactgc
    37081 gtaaaggcga tgaagatcgt atcttaccgg cagtcgataa aggcgatgct ctgacgctcg
    37141 ttgaactgac accagcccag cactttacca agcctccagc ccgtttcagt gaagcatcgc
    37201 tggtcaaaga gctggaaaaa cgcggtatcg gtcgtccttc tacctatgcg tcgatcattt
    37261 cgactattca ggatcgtggc tatgtgcgag tagaaaatcg tcgtttctat gcggaaaaaa
    37321 tgggcgaaat cgtcaccgat cgcctggaag agaatttccg cgagttaatg aactacgatt
    37381 tcaccgcgca gatggaaaac agcctcgacc aggtggcaaa tcacgaagca gagtggaaag
    37441 ctgtactgga tcacttcttc tcggatttca cccagcagtt agataaagct gaaaaagatc
    37501 cggaagaggg gggtatgcgc ccgaatcaga tggttctgac cagcattgac tgtccgactt
    37561 gtggtcgcaa aatggggatt cgcacagcga gcaccggggt attccttggc tgttctggct
    37621 atgcgctgcc gccgaaagag cgttgcaaaa ccaccattaa cctggtgccg gaaaacgaag
    37681 tgctgaacgt gctggaaggt gaagacgctg aaaccaatgc gttgcgcgca aaacgtcgtt
    37741 gcccgaaatg cggcacggcg atggacagct atctcatcga tccgaaacgt aagttgcatg
    37801 tctgtggtaa taacccaacc tgcgacggtt acgagatcga agagggtgaa ttccgcatta
    37861 aaggttatga cggcccgatc gttgagtgtg aaaaatgtgg ctctgaaatg cacctgaaaa
    37921 tggggcgttt cggtaaatac atggcctgca ccaacgaaga gtgtaaaaac acacgtaaga
    37981 ttttacgtaa cggcgaagtg gcaccaccga aagaagatcc ggtgccatta ccggagctgc
    38041 cgtgcgaaaa atcagatgcc tatttcgtgt tgcgtgacgg tgctgccggt gtgttcctgg
    38101 cggccaatac cttcccgaaa tcgcgtgaaa cgcgtgcgcc gctggtggag gagctttatc
    38161 gcttccgcga ccgtctgccg gaaaaactgc gttatctggc cgatgcgcca cagcaggatc
    38221 cggaaggtaa taagaccatg gttcgcttta gccgtaaaac caaacagcaa tatgtctctt
    38281 cggaaaaaga cggaaaggcg actggctggt cagcatttta tgttgatggc aaatgggttg
    38341 aagggaaaaa ataaccttta attctgtcgg gtttttataa acaaagggcc gcgaaagcgg
    38401 cc
//
LOCUS       DADTFG010000026        37261 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.026, whole genome shotgun sequence.
ACCESSION   DADTFG010000026 DADTFG010000000
VERSION     DADTFG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.026"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            103..3861
                     /gene="yfaL"
                     /locus_tag="J1138_001292"
     CDS             103..3861
                     /gene="yfaL"
                     /locus_tag="J1138_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBA8453116.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAANLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGNTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATFGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLAGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAVSLQNGVAGNTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPAQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAVGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQ
                     YVWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHSTGIEEDGSTISDD
                     AAKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4001..4723)
                     /locus_tag="J1138_001293"
     CDS             complement(4001..4723)
                     /locus_tag="J1138_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBA8453117.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4870..7497
                     /gene="gyrA"
                     /locus_tag="J1138_001294"
     CDS             4870..7497
                     /gene="gyrA"
                     /locus_tag="J1138_001294"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBA8453118.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7646..9333
                     /locus_tag="J1138_001295"
                     /pseudo
     CDS             7646..9333
                     /locus_tag="J1138_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            9330..9953
                     /locus_tag="J1138_001296"
     CDS             9330..9953
                     /locus_tag="J1138_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBA8453119.1"
                     /translation="MRHGLLVLICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10097..14491
                     /locus_tag="J1138_001297"
                     /pseudo
     CDS             10097..14491
                     /locus_tag="J1138_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            14492..16141
                     /locus_tag="J1138_001298"
     CDS             14492..16141
                     /locus_tag="J1138_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HBA8453120.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQMSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQQIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKVTEEKST
                     TAVKPSVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTYLQQNADRDGDCLSIPDSSATQRVSASPATVGARTMTAWT
                     QDLIYAGDPVHYHGSRVTEGTLSWRHATAQAGQGERYDQILAFAYPDNNLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLISGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16146..16922
                     /locus_tag="J1138_001299"
     CDS             16146..16922
                     /locus_tag="J1138_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBA8453121.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16996..18180)
                     /locus_tag="J1138_001300"
     CDS             complement(16996..18180)
                     /locus_tag="J1138_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBA8453122.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALATQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFE
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18211..19533)
                     /locus_tag="J1138_001301"
     CDS             complement(18211..19533)
                     /locus_tag="J1138_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBA8453123.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19530..20180)
                     /locus_tag="J1138_001302"
     CDS             complement(19530..20180)
                     /locus_tag="J1138_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HBA8453124.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20180..20842)
                     /gene="atoD"
                     /locus_tag="J1138_001303"
     CDS             complement(20180..20842)
                     /gene="atoD"
                     /locus_tag="J1138_001303"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HBA8453125.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21038..22423)
                     /gene="atoC"
                     /locus_tag="J1138_001304"
     CDS             complement(21038..22423)
                     /gene="atoC"
                     /locus_tag="J1138_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HBA8453126.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22420..24246)
                     /gene="atoS"
                     /locus_tag="J1138_001305"
     CDS             complement(22420..24246)
                     /gene="atoS"
                     /locus_tag="J1138_001305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBA8453127.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24413..27262
                     /gene="rcsC"
                     /locus_tag="J1138_001306"
     CDS             24413..27262
                     /gene="rcsC"
                     /locus_tag="J1138_001306"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBA8453128.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27348..27998)
                     /gene="rcsB"
                     /locus_tag="J1138_001307"
     CDS             complement(27348..27998)
                     /gene="rcsB"
                     /locus_tag="J1138_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBA8453129.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28015..30687)
                     /gene="rcsD"
                     /locus_tag="J1138_001308"
     CDS             complement(28015..30687)
                     /gene="rcsD"
                     /locus_tag="J1138_001308"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBA8453130.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            31426..32553
                     /gene="ompC"
                     /locus_tag="J1138_001309"
     CDS             31426..32553
                     /gene="ompC"
                     /locus_tag="J1138_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBA8453131.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVVDNVTHNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            32665..33720
                     /gene="apbE"
                     /locus_tag="J1138_001310"
     CDS             32665..33720
                     /gene="apbE"
                     /locus_tag="J1138_001310"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBA8453132.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITIGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            33794..34858
                     /gene="ada"
                     /locus_tag="J1138_001311"
     CDS             33794..34858
                     /gene="ada"
                     /locus_tag="J1138_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBA8453133.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            34858..35508
                     /gene="alkB"
                     /locus_tag="J1138_001312"
     CDS             34858..35508
                     /gene="alkB"
                     /locus_tag="J1138_001312"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBA8453134.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGVHPLTTDCRYNLTFRQAGKKE"
     gene            35584..37227
                     /gene="yojI"
                     /locus_tag="J1138_001313"
     CDS             35584..37227
                     /gene="yojI"
                     /locus_tag="J1138_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBA8453135.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         9137 a         9138 c         9974 g         9012 t
ORIGIN      
        1 aatagttatt acatcagggg cagaacttac aaaagaaaaa taccctatat cgcataatgc
       61 gattaaatac gccatcttat agaaaatgat ggttagatga atatgcggat tatctctcta
      121 cgcaaggagt atttatcttt actcccgtca atgattgcat ctcttttctc tgctaacggt
      181 gccgcagcgg tcattgattc atgccaggga tatgatatca aagcgagctg tcaggccagc
      241 aggcaaagcc tttcgggtaa tatacaggac tggagcatcg cggatggaca atggttgatt
      301 ttttcgggta tggtcaataa tgccagcggt ggcgcagtat ttttgcagca cagtgctgaa
      361 tttacgatat caccacaaaa tgaaacaggg atgaccctgt ttgctgataa ctcggttagt
      421 ggcgaatata ataatggtgg ggctatcttt gctaaagaaa actcaacgat aaatcttgcg
      481 aatgttattt tcgacagtaa tgttgcagga ggctatggcg gagcaatcta ttctgccggt
      541 accaatgata ccggtgccgc caatttacgt gtcactaacg ccgtatttca caataacatc
      601 gctaatgacg gcaaaggcgg tgcaatttat accatcaata atgacgtcta tttaagtgat
      661 gatgctttta ataataacca ggcatataca tcaacaagtt atagtgatgg ggatggcggc
      721 gctattgatg ttacggataa tagtacggat aacacacatc tttcaggaaa aacgataatt
      781 aataatacct cctttaccaa taactatgca gaaggttatg gcggagcaat ttataccagc
      841 agcaccacat ccccgtatct tattgatatt tctgttgatg ataattatga ccaaaacaat
      901 ggtgtgatga ttgatgaaaa taacagcgcc tcgggatatg accattcggc gacggcagcg
      961 gcggggggct ttatgtatat cggtcatagt gtggctgaat ttaacattgc cgccgataaa
     1021 acgctggtaa taggcaacac cagcaacgat ggtgcgattg attctcttgc cggaacggga
     1081 gtcatcgtga aagagggagc gggagagtta gtcctcaatg ccgacaataa tgcgtttacc
     1141 ggcgagatga gtatccagaa tggcgaggtg acgctggggc gcagcgatga gttaatgaat
     1201 gtcggcgaca cgcactgtca gagcgatccg caggattgct ttggtctgat ggtcggcagc
     1261 actgttcatt ctgagtatca ggcagaactg aatgttggca atacacaaca aacgtttgtg
     1321 cactcattaa ccggttttgc taatggcatt ttgaatatcg acgcaggcgg caatgttacc
     1381 gtaaaccagg gcggtttttc cggctcgatt cagggtgaag gacagttgac ggtagcgcaa
     1441 gatggcagct atctcctgac aggggcgcag tcgatggcat taaccggcga tattgtggtg
     1501 gaagataacg ccgtattgtc gctagcgggg aatcaggccg atttacgggc aatgcagagc
     1561 gatccgcaat cgatcgtgct aaatggcgga gtattggatc tctctgattt caccacctgg
     1621 gatggcgata gctcatacaa tgacggtctg caaatcagcg gcagcggggg gacagtgatt
     1681 ggcagtaatg atgtcgttga tatcagcagt ggcgatgatt tacatattgg cggcagtgat
     1741 gccagccaga atggcgttta tgtcgtcatt aatgcggggg atcagcgcgt aacgctggcg
     1801 aataacaacg gttatctcgg taatacgcaa atcgcctccg gtacgcttga agtcagcgat
     1861 aattcgcaat tggggaacac aagttacaac cgttcagtga tcttcacaga tccccaacaa
     1921 cacagtgaaa tggatgtgac gactgatgtt gacactcgct cggctacaac aggtcagggc
     1981 aggaatattg aaatgcgcgc tgatggcgaa atacacgttg aggatggcgt agatacgcaa
     2041 tggggcgggt tgatggcgga tagtaccggg caacaactgg atagcgtaag cacgttgact
     2101 aaaagtggcg gcggtacgct ggagttgacc gccagcggca ctgcgacgtc tgcggtacgt
     2161 gtggaggacg gaacgctaaa aggtgaagcg gagaatatta ttccttatgt ttcttcactg
     2221 tgggtggggg aagacggtgt ttttgaaacc gggcaaaatc aggatattcg ttcaatcgat
     2281 gccacctttg gcggcgatat cgacataacc gatggtactg tgttgcgatt gacgcagcag
     2341 gatacgaacc aggcgctgga tgcctcgctg tttagcggcg acggtacgct ggtgaatgcc
     2401 accgatggcg tgacgctggc aggtgagctt aataccaacc ttgaaactga cagcctgact
     2461 tatctttccg acgtgacggt taatggcaat ctgaccaata cgtccggtgc tgtcagtctg
     2521 caaaatggcg tcgctggcaa cacgctgacg gtaaatggtg attataccgg cggcggtacg
     2581 ctactgctcg atagcgaatt aaacggcgat gactcagcaa gtgaccaact ggtattgaac
     2641 ggtaatactg ctggcaacac ggccgtggta attaacccca ttacgggtat tggtgagccg
     2701 acatctacag gtattaaagt agttgatttc gcagctgatc ccgcgcagtt tcaaaacaat
     2761 gcgcagttca gtctggcagg aagcggctac gtcaatatgg gggcgtatga ctacacgctg
     2821 gtggaagata acaacgactg gtatctgcga tcacaagaag tcaatccgac gccgccgcct
     2881 gatccagacc cgactccaga tcctgatccc acgccggatc ccgacccaac ccccgacccg
     2941 gaacctacgc ctgcctacca gccggtgttg aatgccaaag ttggtggata ttttaataac
     3001 ctgcgggcgg caaatcaggc atttgtgatg gagcgacgcg atcacgctgg tggcgatggt
     3061 cagacgctga atttacgcgt tatcggcggg cgttatcatt acacggcggt agggcagttg
     3121 gctcagcatg aagacacttc tacggtgcag cttagcggca acctgtttag cgggcactgg
     3181 ggtgatgatg gcgagtggat gcttgggatt gttggtggct acagcgataa ccagggcgac
     3241 agccgctcga atatgaccgg aactcgcgcc gataaccaga accacggtta tgccgttggg
     3301 ctgacctcaa gctggtatca gcacggtaat cagaagcagg gggcctggtt ggatagctgg
     3361 ctgcaatatg tgtggtttaa caatgatgtt tccgaacaag acgatggcgt ggatcattac
     3421 cactcgtcgg ggattatcgc ctcgctggaa gccggttatc agtggttacc ggggcatggt
     3481 gtggtgattg aaccgcagac gcaggtgatt tatcagggcg tgcagcagga tgattttacc
     3541 gccgctaacc atgcgcgcgt gtcacaatcg cagggtgatg atattcagac gcggctgggt
     3601 ttacacagcg aatggcgtac tgctgttggt gtcacgccaa cgttagatct gaattattat
     3661 cacgatcccc attcgacggg aattgaagaa gatggcagca ctatcagtga cgatgcggcg
     3721 aagcaacggg gtgaaataaa agtgggaata acgggcaata tcagtcagcg agtttcgctg
     3781 cgtggtagcg tggcgtggca gaaagggagt gatgattttg cccagacggc agggtttttg
     3841 tcgatgacag tgaaatggta atcgctgtct gaccggcact gttgccggat gcggcgtaaa
     3901 cgccttatct ggcctacggg gagcatttgt aggcctgata agacgcggca agcgccgcat
     3961 caggcttggc tcggttgccg gatgaatcca aaccacaacc tcacttattc tgcgtgtgca
     4021 gcatatagtt cacatccacg ccggggccga gcttaaagga attagtgata gggttgtaat
     4081 gcagcccggt catatgccgt tctttaagac tggtctgatc cacccagccc agcaattctg
     4141 ccggtttaat aaacttcttc acatcatgtg tacctttagg caccatgcgc aaaatatatt
     4201 ccgcaccaac caccgccatc agccatgact tgccgttgcg gttaagcgtc gagaaaaaga
     4261 catcgccgcc cggtttcacc agttgcgcac aggctctgac cactgactga ggatcgggga
     4321 cgtgctccag catctccata caggtcacca cgtcatactg tccggcatgt tttgccgcgt
     4381 gctcttccac ggtttcctgc acgtaatcca cctgaatgcc gctttccagt gcgtgcagtt
     4441 ttgccacctg caatggctca aagcccatat ccagaccggt caccgtcgcg ccctcccgcg
     4501 ccatactctc ggccagaatg ccgccgccac aaccgacatc gagcaccttt ttgccaaata
     4561 aaccgccagc acgctcggca atatagccca gacgcagcgg gttaatgcgg tgcagcggtt
     4621 tgaactcacc ttcaagatcc caccagcggg aggcgacggc ttcaaattta gcgatctctt
     4681 cgtggtctac gttgtggttt accggcgatt tttcggcatt cattggcact tctactccgt
     4741 aattggcaag acaaacgagt atatcaggca ttggatgtga ataaagcgta taggtttacc
     4801 tcaaactgcg cggctgtgtt ataatttgcg acctttgaat ccgggataca gtagagggat
     4861 agcggttaga tgagcgacct tgcgagagaa attacaccgg tcaacattga ggaagagctg
     4921 aagagctcct atctggatta tgcgatgtcg gtcattgttg gccgtgcgct gccagatgtc
     4981 cgagatggcc tgaagccggt acaccgtcgc gtactttacg ccatgaacgt actaggcaat
     5041 gactggaaca aagcctataa aaaatctgcc cgtgtcgttg gtgacgtaat cggtaaatac
     5101 catccccatg gtgactcggc ggtttatgac acgatcgtcc gtatggcgca gccattctcg
     5161 ctgcgttaca tgctggtaga cggtcagggt aacttcggtt ccatcgacgg cgactctgcg
     5221 gcggcaatgc gttatacgga aatccgtctg gcgaaaattg cccatgaact gatggccgat
     5281 ctcgaaaaag agacggtcga tttcgttgat aactatgacg gcacggaaaa aattccggac
     5341 gtcatgccaa ccaaaattcc taacctgctg gtgaacggtt cttccggtat cgccgtaggt
     5401 atggcaacca acatcccgcc gcacaacctg acggaagtca tcaacggttg tctggcgtat
     5461 atcgatgatg aagacatcag cattgaaggg ctgatggaac acatcccggg gccggacttc
     5521 ccgacggcgg caatcattaa cggtcgtcgc ggtattgaag aagcttaccg taccggtcgc
     5581 ggcaaggtat atatccgcgc ccgtgccgaa gtagaagttg acgccaaaac cggacgtgaa
     5641 accattatcg tccacgaaat tccgtatcag gtgaacaaag cgcgcctgat cgaaaaaatt
     5701 gcggaactgg taaaagaaaa acgcgtggaa ggcatcagcg cgctgcgtga cgagtctgac
     5761 aaagacggta tgcgtatcgt gattgaagtg aaacgcgatg cggtcggtga agttgtgctc
     5821 aacaacctct actcccagac ccagctacag gtttctttcg gtatcaacat ggtggcattg
     5881 caccatggtc agccgaagat catgaacctg aaagatatca tcgcggcgtt tgttcgtcac
     5941 cgccgtgaag tagtgacccg tcgtactatt ttcgaactgc gtaaagctcg cgatcgtgct
     6001 catatccttg aagcattagc cgtggcgctg gcgaacatcg acccgatcat cgaactgatc
     6061 cgtcatgcgc cgacgcctgc agaagcgaaa accgcgctgg ttgcaaatcc gtggcagctg
     6121 ggcaacgttg ctgcgatgct ggaacgtgcg ggcgacgatg ctgcgcgtcc ggaatggctg
     6181 gagccagagt tcggcgtgcg tgatggtctg tactacctga ccgaacagca agctcaggcg
     6241 attctggatc tgcgtctgca gaaactgacc ggccttgagc acgaaaaact gctcgacgaa
     6301 tacaaagagc tgctggatca gattgcggaa ctgttgcgta ttcttggtag cgccgatcgt
     6361 ctgatggaag tgatccgtga agagctggag ctggttcgtg aacagttcgg tgacaaacgt
     6421 cgtactgaaa tcactgccaa cagcgcagac atcaacctgg aagatctgat cacccaggaa
     6481 gatgtggtcg ttacgctctc tcaccagggc tacgtgaagt atcagccgct ttctgaatat
     6541 gaagcgcagc gtcgtggcgg gaaaggtaaa tctgccgcac gtattaaaga agaagacttt
     6601 atcgaccgac tgctggtggc gaacactcac gaccatattc tgtgcttctc cagccgtggc
     6661 cgcgtctatt cgatgaaggt ctaccagtta ccagaagcta ctcgtggcgc gcgtggtcgt
     6721 ccgatcgtca atctgctgcc gctggaacag gacgaacgta tcaccgcgat cctgccggtg
     6781 accgagtttg aagaaggcgt gaaagtcttc atggcgaccg ctaacggtac tgtgaagaaa
     6841 accgtcctca ccgagttcaa ccgtctgcgt accgccggta aagtggcgat caaactggtt
     6901 gaaggcgatg agctgatcgg cgttgacctg accagcggcg aagacgaagt aatgctgttc
     6961 tccgctgaag gtaaagtggt gcgctttaaa gagtcttctg tccgtgcgat gggctgcaac
     7021 accactggtg ttcgtggtat tcgtttaggc gaaggcgata aagtcgtctc tctgatcgtg
     7081 cctcgtggcg atggcgcaat cctcaccgca acgcagaacg gttatggtaa acgtaccgca
     7141 gtggcggaat acccaaccaa gtcgcgtgcg acgaaagggg ttatctccat caaggttacc
     7201 gaacgtaacg gtttagtggt tggcgcggtg caggtagatg actgcgacca gatcatgatg
     7261 atcaccgatg ccggtacgct ggtacgtact cgcgtttcgg aaatcagcat cgtaggccgt
     7321 aacacccagg gcgtaatcct tatccgtact tcggaagatg aaaacgtggt gggtctgcaa
     7381 cgtgttgctg aaccggttga cgaggaagat ctggatacca tcgacggcag tgccgcggaa
     7441 ggggacgatg aaatcgctcc ggaagtggac gttgacgacg agccagaaga agaataattt
     7501 catcgcttca tgccaaaagg gagccatctc ccttttttga attgaaaagt ccaggctgta
     7561 aagtctgggc ttttgtcgta ttagggcgcg gtaaagtttg gctgtgcccg taaaaaatgg
     7621 ctggctatac acaaggaatg tggcaatgag tggtgaaaaa aaggcgaaag gctggcggtt
     7681 ttatggcctt gttggttttg gggcaatagc gctgctatcc gctggcgtat gggcgttgca
     7741 atatgctggc agtgggccaa aaaaaacgtt gtcgccactg gtggtgcaca acaatctgca
     7801 aatcgatctc aatgagccgg acctctttct cgacagcgac tctctgagcc agcttcccaa
     7861 agatctcctt accattccgt ttctccacga tgttctgagc gaagatttcg ttttctatta
     7921 tcagaatcat gccgatcgtc tgggcattga aggcagcatt cgccgcattg tctatgaaca
     7981 cgatctcacg ctgaaagata agctcttttc gtcactctta gaccagcccg cgcaggcggc
     8041 actgtggcat gataaacaag gtcatctttc acattatatg gtgcttatcc agcgcagtgg
     8101 tttaagcaaa ctgctggagc cattgttgtt tgccgctacc agcgacagcc agttaagcaa
     8161 gacggaaatc agtagcatca agataaatag tgaaactatt cctgtttatc agttgcgcta
     8221 taacggcaat aacgtcctga tgtttgcgac ttatcaggac aaaatattgg cgttttccag
     8281 ttcggatatg ctgtttaaag atgaccaaca ggataccgaa gctacggcga tcgcaagtga
     8341 tttgttgagt ggtaaaaacg ctgggaaacc cgctttggcc tggaagcgcg tactgccgaa
     8401 aaaacgccag tacgccagcg catcgtagtc agcgccaggt tgctggggtt tggctaccag
     8461 cgattaatgc cttcttttgc tggcgtgcgc ttcgaaatgg gtaacgacgg ctggcatagc
     8521 tttgtggcgt taaatgatga atccgccagc gtagatgcca gtttcgattt tacgccggta
     8581 tggaacagta tgcctgccgg agccagcttc tgtgtggcgg tgccgtattc acacggtatt
     8641 gccgaagaga tgctttcgca catcagccag gaaaacgaca agctgaatgg ggcgttagac
     8701 ggtggcgccg ggctgtgctg gtatgaagac tcaaaattac aaaccccgct atttgttggt
     8761 cagtttgatg gcactgccga acaggcgcaa ttgccaggga aactgtttac gcaaaatatt
     8821 ggtgcgcacg aaagcaaagc gccagaaggt gttttgccgg taagccagac tcagcagggc
     8881 gaagcgcaaa tctggcgtcg cgaagtgagt tcccgatacg gtcagtatcc gaaagcgcag
     8941 gcggcgcaac ccgatcaatt aatgtcggat tattttttcc gcgtgtcgct ggcgatgcaa
     9001 aacaaaacgc tgcttttctc cctcgatgac acgctggtta ataacgccct gcaagcactg
     9061 aataaaaccc gcccggcaat gatggatgta atacccactg atggcatcgt tccgctctat
     9121 atcaatccac aaggaatggc gaaactgctg cgtaacgaaa cgctgaccag tctgccgaag
     9181 aatctcgaac cggtttttta taacgccgca caaactttat taatgccgaa gctggacgct
     9241 ttatctcaac aaccgcgtta tgtcatgaag ctggcccaga tggaacccgg tgccgcctgg
     9301 cagtggctac ccataacctg gcaaccgcta tgagacacgg gctgctggtg ctgatttgct
     9361 ggctgtattg tgttgttgcc catagtgaaa tgctaaatgt cgaacaatcc gggctgtttc
     9421 gcgcgtggtt tgtgcgcatt gcacaggaac agctccgcca ggggccaagc ccacgctggt
     9481 atcaacagga ttgcgcgggc ctggtgcgat ttgcggcgaa cgaggcgctg aaagttcacg
     9541 acagtaaatg gctaaaaagt aacggtttat ctaaccagta tttgccgcca gagatgacgc
     9601 taacacctga acagcgtcaa ctggcgcaaa actggaatca ggggaatggg aaaaccggcc
     9661 cctatgtgac cgcgattaat ttgattcagt acaacagcca gtttattggc caggacataa
     9721 accaggcact gcctggcgat atgatttttt tcgatcaggg cgatgcccag cacttaatgg
     9781 tctggatggg gcgttacatc atctaccaca ccggaagcgc cacgaaaact gacaacggaa
     9841 tgcgcgcagt cagtctgcaa caacttatga catggaagga cacccgatgg atacccaacg
     9901 attccaatcc caatttcatt ggcatttatc gtttaaattt tctggcgcga tagcagcgtg
     9961 cttgtccctg tctcttgtgg gaacagggct tgctaatgct gatgattcgc ttccttccag
    10021 taactatgcg ccgcccgccg ggggaacatt ctttttgctt gctgacagca gttttagccg
    10081 cagtgaagag gcgaaagtgc gactggaagc gccggggcgt gattatcggc gctatcagat
    10141 ggaagagtac ggcggcgtgg acgttcgcct gtatcgtatt cctgacccga tggcattttt
    10201 gcgccagcag aaaaacctgc atcgcattgt ggtgcaaccg caatatctgg gcgacggact
    10261 gaacaatacg ctgacctggc tgtgggataa ctggtacggc aaatctcgcc gcgtgatgca
    10321 gcgtactttc tcttctcagt cacggcagaa tgtgactcag gcgttacccg aattacagct
    10381 cggcaatgcc attattaagc cttcccgtta tgtacagaac aaccagtttt caccgctgaa
    10441 aaaatatcca ctagtggaac agttccgtta cccactatgg caggctaaac cggtcgagcc
    10501 gcaacaaggg gtaaaactgg aaggcgcatc cagcaatttc atttcgccgc agccgggcaa
    10561 catctatatt cctctcggca aacaagagcc gggactgtac ctcgtcgagg cgatggtggg
    10621 tgggtatcgg gcgacgacgg tggtgtttgt ttccgatacc gtggcgctta gcaaagtgtc
    10681 aggtaacgag cttctggtgt ggaccgcggg gaaaaaacag ggtgaagcga agaccggctc
    10741 agagatcttg tggactgacg gtcttggcgt gatgacccgc ggtgtgaccg atggcagcgg
    10801 taccttgcag ttacaacata tatcgccaga acgttcatat attctgggta aggatgctga
    10861 aggcggcgtt tttgtctccg agaacttttt ctacgaaagc gaaatctaca acacccgctt
    10921 gtatatcttt accgatcgcc cgctatatcg cgcaggcgat cgtgtcgatg ttaaagtgat
    10981 gggccgcgag ttccacgatc cgttgcattc atcccccatc gtcagcgcac cggcgaagct
    11041 ttcggtgctg gacgccaacg gcagtctgtt gcaaaccgtc aatgtcacgc tggatgcgcg
    11101 taatggcggg caggggagtt tccgcctgcc agaaaatgcc gtcgccggag gttatgagtt
    11161 acgtcttgct taccgcaaac aggtctatag cagcagtttt cgcgtggcaa actacatcaa
    11221 accccatttt gagattggtt tagctctcga taaaaaagag ttcaaaactg gcgaagcggt
    11281 cagcggcaaa ctgcaactgc tctacccgga tggcgagccg gtaaaaaatg cccgcgtgca
    11341 gttaagtttg cgcgctcagc aattatcaat ggttggtaac gatttgcgtt atgccggacg
    11401 tttcccggtg tcgctggaag gcagtgaaac ggtttccgac gataatggtc atgtggcgtt
    11461 gaatctcccc gccgccgata aaccgagccg ttatttgtta accgtctccg ccagtgacgg
    11521 cgcggcgtat cgagttacca ccaccaaaga gatcctcatt gaacgtggct tggcgcatta
    11581 ctctttaagt accgccgcac aatacagtaa tagcggcgag tcggttgtgt tccgttatgc
    11641 cgcgctggaa tcttcaaaac aggttcctgt tacttatgaa tggttgcgtc tcgaagaccg
    11701 cacgagccac agcggagagc tacagtcagg cggcaaatcc tttaccgtca atttcgctaa
    11761 acctggcaac tacaatctga cattacgcga taaagacggc ttaattctcg ccgggttaag
    11821 ccatgcagtc agcggtaagg gcagcacgtc gcatactggt acggtagata tcgtggcaga
    11881 taaaacactg tatcagcctg gcgaaaccgc gaagatgctg attaccttcc cggagccaat
    11941 tgacgaagca ttattgacgc tggaacgcga tcgcgtggaa cagcagtcgc tgctatcgca
    12001 tccggcaaac tggctaacgc tacaacgttt aaacgatacc cagtatgaag cccgcgtacc
    12061 ggtgagcaat tcctttgcgc ctaacatcac tttttcggtg ctgtataccc gtaacggtca
    12121 gtacagtttt cagaacgccg ggattaaagt tgccgttccc cagctggata tccgggtgaa
    12181 aacggacaaa acccattacc agcctggtga actggtcaat gtcgaattaa cctcgtcgct
    12241 gaaaggtaaa cctgtttctg cgcagttaac ggtaggcgtg gtcgatgaaa tgatctacgc
    12301 gctgcaaccg gaaatcgcgc cgaatatcgg caaatttttc tatccgctgg ggcgtaacaa
    12361 tgtgcgtacc agctccagcc tgtcatttat cagctacgat caggcgctct ccagcgagcc
    12421 ggttgcgcct ggcgcgacta accgcagcga gcggcgagta aaaatgctgg aacgtccacg
    12481 gcgtgaagag gtggataccg cggcctagat gccgtcactc acaaccgata aacaaggcaa
    12541 agcgtatttc acgttcctga tgcctgattc gttaacccgc tggcgcatca ccgcgcgtgg
    12601 gatgaacggc gacgggctgg tcgggcaggg gcgtgcttat ctgcgttcgg aaaaaagtct
    12661 ctacatgaaa tggagtatgc caacggtgta tcgcatgggc gacaaaccgg cggcaggact
    12721 gtttatcttc agtcagcagg ataacgaacc ggtggcgctg gtgactaaat ttgcaggtgc
    12781 tgagatgcgc cagacgctga cgctgcacaa aggggcgaat tatatttcgc tgacgcagaa
    12841 tattcagcaa tctggcttgt taagtgcaga actgcaacaa aatgggcaag tacaggacag
    12901 cattagcaca aaactgtctt ttgtggataa cagctggccc gttgaacaac agaaaaacgt
    12961 catgttcggc ggtggcgata atgcgctgac gttgcccgag caggcgagca atatccggct
    13021 acaaagtagc gaaacgccgc aggagatttt ccgtaacaat cttgatgcgt tagtcgatga
    13081 accgtggggg ggcgtgatca acaccggtag ccgtctgatc ccgctcagtc tcgcctggcg
    13141 ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt cgtcagatga ttcaggataa
    13201 ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc tttacctggt ggggtgaaga
    13261 tggcaatggc gacgccttcc tcacggcatg ggcatggtac gccgactggc aggccagcca
    13321 ggcgctcggc gtaacgcagc aaccggaata ctggcaacat atgctcgaca gttatgccga
    13381 gcaggcggac aacatgccgt tattgcatcg ggcgctggtg ctggcgtggg cacaggagat
    13441 gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa gctatcgccc ggcgcggaac
    13501 taaaactaaa gatttctctg aggcagacac cagcgatatc aatgacagca tgatcctcga
    13561 tacacctgaa tctccactgg cagatgcggt ggcaaacgtc ttaaccatga cgttgctgaa
    13621 aaaagcgcag ttgaagtcca ctgtgatgcc acaggttcag caatatgcgt gggataaagc
    13681 ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg ctcaatagcg ggggcgacgc
    13741 tacccaggcg gctgctattt taagtggttt gaccgctgag caatctacta ttgagcgcgc
    13801 gctggccatg aactggctgg cgaaatatat ggcgacaatg cctccagttg ttttgcctgc
    13861 gcctgcgggc gcatgggcca aacataagtt aactggaggg ggcgaatact ggcgttgggt
    13921 tggtcagggc gtgccggaca ttctctcttt tggtgacgaa ttatcgccgc aaaatgtgca
    13981 ggtccgctgg cgtgaaccgg caaaaacggc tcaacaaagt aacattccgg tgaccgttga
    14041 acgccagttg tatcggctta tccccggtga agaagagatg agctttactc tgcagccggt
    14101 gaccagcaat gagattgaca gcgatgcgct gtatctcgat gaaattacgc ttaccagcga
    14161 gcaggatgca gttctgcgct atggtcaggt ggaagtaccg ctcccgccgg gagccgacgt
    14221 tgagcgcaca acatggggca tttcggtcaa taaacctaac gctggaaaac agcaggggca
    14281 attgctggaa aaagcgcgta atgaaatggg cgaactggcc tatatggtgc cggtgaaaga
    14341 actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag ggcaattcgt
    14401 tctgcctcct gctcgttatg tgcgttccta tgcacctgca cagcaaagtg ttgcggcagg
    14461 gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg tctggctatt
    14521 ggcgttggtt actctgccaa cgcttgcgga ggagacgccg ttacaactgg tgctgcgcgg
    14581 tgcgcagcac gatcaactgt atcagatgtc gtcatcaggc gtgaccaaag tctcagcatt
    14641 acctgacacg ctcaccacgc cgttagggag tttatggaaa ctgtatgttt acgcctggct
    14701 ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc cggaagaggt
    14761 ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac gctcctgtgg
    14821 actctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc aatactggca
    14881 acagcgtcag gcacctgcgt ggttagcgtc tcttactaca ctgaagccag aaacctcggt
    14941 aacggttaaa tcgctgcttg attcattagc gacgttaccc gcgcaaaaca aagctcagga
    15001 agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca tgctcggtag
    15061 tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacagcaaa tccgtcaggg
    15121 tggttttgcc ggctggttaa ccgatggtac accgctgtgg gttactggca gtggcaccag
    15181 caaaacggta ctgacccgtt atgcgacggt gcttaatcgg gtattgccag tgccgacgca
    15241 agttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgttatccgc tgaagaaagt
    15301 tactgaggaa aagagtacga cggcagtcaa accaagcgtg ctcaatgggc gctatcgtgt
    15361 gacgtttgcc aatggtaacc acatgacatt tgtcagccac ggtgagacaa cgttactgac
    15421 ggtaaagggc aaactgaaat tacagtcgca tctcgatcgt gaagagtacg ttgccagagt
    15481 tctggatcgc gaggcgaaaa gtacgcctcc tgaggcagcg aaagccatga ccgtggcgat
    15541 tcgcacgtat ttacagcaaa acgccgatcg cgatggtgat tgcctgagca tcccggacag
    15601 tagcgctacc cagcgcgttt cagcttcgcc agccactgta ggcgcgcgga cgatgaccgc
    15661 ctggacgcag gatctcatct atgcgggcga tccggttcat tatcatggca gccgggttac
    15721 cgaagggacg ctttcctggc ggcacgctac tgcccaggcg gggcagggag agcgttacga
    15781 tcagatactt gcgtttgctt atcctgacaa caaccttagc cgctggggag cgccacgctc
    15841 aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaatgc cgcagtggcg
    15901 gcgtatatta caaggtgaga cgggatacaa cgaaccagac gtgtttgcgg tctgtcgttt
    15961 aatctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc gtaacttctt
    16021 tacgcttcag gatcggctcg atttaaccca tgagtatctg cacctggcct ttgatggtta
    16081 tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat taatggacta
    16141 atgttatgcg aaagattttt cttccgttgt tactggtggc gctttcgcct gtcgctcaca
    16201 gtgaaggtgt gcaggaagtc gagattgatg caccgctctc tggctggcat ccggcagagg
    16261 gtgaagatgc cagtttttca caaacgatca attacccggc gtcgtccgtc aacatggcag
    16321 atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct gcggcgggta
    16381 aagttcagca gggccggctg gtggtcaatg gtgccagtat gccgcagaga attgaatcag
    16441 atggttcttt tgcacgccct tatatcttca ctgaaggcag caacagcgtg caggtcatca
    16501 gcccagatgg gcaaagccga caaaaaatgc agttttactc aacgccgggt gctggggcga
    16561 ttcgcgcacg tttacggctg gttctctcgt gggatacgga caataccgac ctcgatcttc
    16621 atgtcgttac gcctgacggt gaacatgcct ggtacggtaa caccgtgctg aaaaacagtg
    16681 gtgcactgga tatggatgtc acgacagggt acggccccga gattttcgcc atgccagcgc
    16741 cagttcacgg ccgttatcag gtgtatatca actactatgg cggacgcagc gaaacggaat
    16801 taaccaccgc ccaactgacg ctgatcaccg atgaagggtc ggtcaatgag aaacaggaaa
    16861 cttttattgt accgatgcgt aatgctggcg aactgacgct ggtgaaaagt tttgactggt
    16921 gattaatgag gttatcttta ctgtcaatgt tcaagaaaac gcccgatgac tttcgctatc
    16981 gggcgttttt acatgttaat tcaatcgttc aatcaccatc gcaattccct ggccgccacc
    17041 aatgcacagt gttgccagcc ccagcgtttt atcgcgtgcc tgcattgcat gtaatagtgt
    17101 gaccagaata cgagcaccac tggcaccgat aggatgcccc agcgcgatgg ccccgccgtt
    17161 gacattcact ttctcaggct caaagcccag ggttttccca acggcaagga actgtgcagc
    17221 aaatgcttca ttagcctcaa tgagatcaat atccgccagt tgcagccccg ccagttgtaa
    17281 cgctttttgc gtggcaggta ctggccccat acccatcaat gcggggggca cgccaccgct
    17341 ggcataactt ttaatgcgtg ccaggggatt caggcctgct gccagcgccg cagattcttc
    17401 cataatcacc agagcggcag caccgtcgtt aatacctgac gcgttcccag cggtgactgt
    17461 tcctgcttta tcgaaggccg gacgcaatgc gcctaacgct tcagtcgtag aatccgcttt
    17521 cgggaattcg tcttgactga agacgaaggt tttcttccgg gtgacaacat ttaccgggac
    17581 gatttcggct gtaaaagcac cggactcaat tgcggctgcc gctttacgct gtgaatgtag
    17641 cgccagttca tcctgcattt ctcgggtaat tccgtactct ttggccacgt tctcggcggt
    17701 aatccccata tgataaccat gggtggcgca catcagacca tcgcgcagga ttacgtcata
    17761 aacctgtccg tctccaagac gataaccaga gcgtgctttt gcatcgagta agtagggcgc
    17821 taaactcata ttttccatac cccccgccac aatgctttgc gcctgacctg cctgaattgc
    17881 ctgggtggca agcgccacac ttttaagacc cgaaccacac actttattga ccgtgaatcc
    17941 gcacaccgtt tctgccagcc cgctttttaa cagtgcctga cgtgccggat tttgccccaa
    18001 cccggcttgc aacacgttac ccataatcac ttcatcaatg tgtagtgaat cgatttttgc
    18061 acgttcaatg gcggctttaa ttactgtcgc ccccaggtcg atggcgctgg tggaagcgag
    18121 tgaaccgtta aaactaccga tagcagtacg taccgcactg acgatgacac aatttttcat
    18181 tttttattcc ttctgtctgt agggttgccg tcagaacagc gttaaaccaa tgacgaaaat
    18241 gacgccggag aagagcaggg cagtgatgca atagcccatg atgtcgcgga caccgagtcc
    18301 ggcgattgcc agtgctggca gcgcccagaa tggttgtgcc atgttcatcc attgctcgcc
    18361 gtaggcgatc gccattaccg atttaccgag atcagcgccc agcgcctggg ctgcgggtat
    18421 cacgaaaggt ccctgaataa cccagtgacc gccgccagac ggaacggcga agttaatcag
    18481 tgcagaacta aaaaaggtca ttaccgggaa ggtgtctttg ttcgcaacat tgatgaagaa
    18541 ttcggtaatg agtccgccca gaccggaatg ctccatcatc agttggatcc cagcgtagaa
    18601 ggggaattgc accagaatac cggcagtact gcgtgctgcc gcgctgatag cacgcatata
    18661 agccattggc gttttatgta gcagcagacc cgcaatcata aacatcaggt tgacggtatt
    18721 gatggtgatg ttgaagccat gttcgctgaa gtacatcgcc aggtaagcga taccgagtgc
    18781 gccgatgatc aacgtcagaa tacggctttc ttccagtcgc tcggacggtg gagcatcttt
    18841 cggtagttgc ttttgaaaat cagcctcttc catgagtagt ttcggatcga tactcaccac
    18901 gtcagacggt tttggcatca tcatgcgggt gataaatggc atcaccacaa tcaacgccac
    18961 agtgatgaaa atattaaaac cactgaacag agtatcgccc accgggatca gcccggcgat
    19021 atgctcaacc gggttgcccg gtgttgcagc caacagaggc attgagccag agaaaccgcc
    19081 accccaggtg agaaaaccaa tgtaggcgca ggcaatgagc aacggataat cagaaccggg
    19141 tactcgccgg gcgacttcac gggcgaacat tgcaccgaca accaaaccaa atccccagtt
    19201 gatgacacaa gcaactgaac cgaagaaagt aaccagcatg acgccctgta cgggcgtctt
    19261 tgcggcggag gcggcagtac gcagcaaact tttcaccggc gcggagctgg caagggcatg
    19321 accggtaacg atgataagcg ccatctgcat accaaacgcc agcaagttcc agaaaccgtc
    19381 accccacatt ttcaccatgc tgatcggcgt ttgtggtgtt aaccaaagcg cgatcacgaa
    19441 tgttagcaat gtcagcaaca tggcaaagat cagtggatcg ggaagccacc ggctgacaaa
    19501 acgcgtcata aaacgcgata tgcgaccaat cataaatcac cccgttgcgt attcagatcg
    19561 gcagcgactt caaaccgagc ttctgttttg gcacgcacgg tggctaaatc acacccgtcg
    19621 gcaatttcgg tgagccacat tttgccgtca ataaaacgaa agacagccag ttcagtaacc
    19681 agcatatgca ccgcatgttg cgcagtgagt ggcatggtgc agcggcgcaa aatttttgct
    19741 gaaccatctt tggcgcaatg ttccatggcg atgatcactt tgcgcgaccc ggtcaccaga
    19801 tccatcgcgc cccccatacc gggcaccatt ttcccaggca ctacccagtt cgcgaggttt
    19861 gcttcttcgt ctacttgcaa accaccgagc acgcaggcat caatatgacc gccacggatt
    19921 agcgcaaatg acatggcgct atcaaacatg gctgcgccgg gtaaaatgcc gcacggttgt
    19981 ccgccagcgt tcaccagatc tggatgcgct gttgtgaccg ggcctaaacc gaggaagccg
    20041 ttttctgatt gcagagtgat atgaataccc tccggtaaat aattggcgac cattgtgggt
    20101 aaaccgatcc ctaagttaac gatgtcacca tcacgaagct cttgcgccac acggcgcgca
    20161 atacgttgtt tcgcatccat tatttgctct cctgtgaaac gatgatgtgg tcgataacgg
    20221 caccaggggt gacaatatgg tcaggttgca gctcgccggt ttcgaccagt tcatccggct
    20281 ctaccagcgt gatatcagcc gcaagggcta tcagggggtt aaagttgcgg gcgctaaggt
    20341 gataggtcag gttgccaagt gtgtcgcaac gatgagcgcg aattagcgcc aggtcggcgc
    20401 gcagcgggcg ttcgagcagc caggttttac cgtcgagtgt cagtgtctgt ttgccttcct
    20461 ctacgacggt gccgacacca gttggggtga gaaaaccacc aagtccagct ccgccacagc
    20521 gaatttgctc gattagcgta ccttgcggca ccagaacgac gtccatctca ccagatatca
    20581 tgcgccgacc tgtttccggg ttggtgccga tatgtgaagc aatcactttg cggactcgac
    20641 cattgacgat gagcggaccg atgcctgtat caacaaacgc ggtatcattg gctatcaatg
    20701 tcaggtcgcg aacaccagat tccagtaatg cttcaaccag gcgggatgga gtgccaatcc
    20761 ccataaatcc gcccaccatg atggtcatgc cgtcacgaaa gaagccggtg gcgtcttgta
    20821 atgtcatcaa ttttgttttc attttttatc cctcttgcat acaccgttgt gggtacagtc
    20881 agggaatagc aaggggagtg ccagaatgag attattgaat gttgttattt aactaattga
    20941 atagaaaagg ataaattaga tggggattca taaggagaat gagcggctat gcagaaaatt
    21001 gcgcactgtg caaatttctg catagcaagt tttggcgtta tacatccgcc ggatcgatac
    21061 cgtattcctg gagtttatac atcaatgcac ggcgactgat gcccagcatt aaagcagtgc
    21121 gggttcggtt tccttcttgt tgttccagca cttccataat gatgcgtttt tcgacgcgtt
    21181 taatttcctc ttttaaatta cgctcaccga caggggctgt ttttgcttca ccagcattac
    21241 agactggctg acgaatctgc ggcggaagat cctcagaaaa aatgatcggg cctgaattca
    21301 tcacgacggc gcgttcaata acgttggaaa gctctcgaat atttcccggc catgaccagg
    21361 cggtaagcag tgacattgcc atcggatcga tgtcgataat atcgcgctga ttctcactac
    21421 tgaatttttg caaaaagtga ttagctaaca gggaaatatc ttcccggcga tcgcgcagag
    21481 gcggcagtat taaatgaata acgttaaggc gataaaagag atcttcacgg aaggtgcctt
    21541 ctttcaccat tgcctgcaag tcgcggttgg tggcagcaat gatgcggata tcaactttta
    21601 tggtctgatg gccgccaatc cgttcaaatt cccgttcctg cagaatgcgt agtaatttgg
    21661 cttgcagtac cagcggcatt tcgccaattt cgtcgaggag cagagtacct tcgttggcac
    21721 gttcaaataa tccctgacgc aaggtttgtg caccagtaaa tgcacctttt tcatgaccaa
    21781 acagttcact ttcgagcaac gattccggca gcgccgcgca gttgacttta atgaacggcc
    21841 cctttgcccg ccgcgaattg tagtgaatcg ctctggcaat caactctttc ccagtgccgc
    21901 tttcaccgct aatcaagacg ctggcctgag aaagggcaat tttggcggtg tctttgcaga
    21961 tgtccatcat cgccgggctg ttggtgagaa tgtgccccca ttgccagctg gtgctcagtg
    22021 cctggtgcag atgacggatc tcttttttca ttgactggag ttgtaaagcg cgctgaacga
    22081 ttaaattcaa ctcatcgaga tcaaacggtt tgataacata gtcgaaggct ccgcagcgta
    22141 gtgcttcgac ggcggtttcc acttcagcat aggccgtcat cagaataacg ggtgtccggg
    22201 tctcatggct gcgcatctcc tttagtgcct tgatgccgtc catctctggc atgcggatat
    22261 ccatcaacac cacgtcaggg tgaatatcgg caaacaggtg taatgctgtg cgcccgttgt
    22321 tcgcacaatg tgtttcgaat ccttgtagtg caaaagcggt gctcagcata cggcgaacat
    22381 tatcttcatc atccacaata aggatgcgat taatagcagt catacagtct gatttccctg
    22441 cgggttgatc ggtaaaataa gcgtgaaggt tgcgccgtag cccggcaaac tggcgacgcg
    22501 aatatcaccc tgatgggcat taatgatgcg ttgacttaac gccagaccaa gcccggttcc
    22561 tgaggctttg gtagtgaaaa agggatcgaa gatctttttt tgcagcgaga gatcaatgcc
    22621 actgccgttg tcctctatcg aaatggcctg ttgcgagtcg ctatactgcc aggttcgaat
    22681 gcgaattttc cctcgtgcac tgatagcctg gacggcattg atcaggatat tcagtagtac
    22741 ctgtttgagc agttcacgat cggcgttaat cgggcttaat tcattatcca gttcgcttat
    22801 gaagtcgacc cgcgcttgta cgccggcggt ttgtaccagt accagagttt cttcaaccaa
    22861 tgcattgagg ctgacttgtt gccattgact gtggcgtgga cgtgaaaatt cgagcaattg
    22921 ctgaataact ttgttaattg aatcgatttc tttgagtact acggacagat attcctgatg
    22981 tattgggtca cgggtttgtt ggcgcaagat ctgtacataa ccacgaatag ccgttaacgg
    23041 attacgtact tcatgcgcca cgccagccat cagctcaccc agtgtggcga ggcgttctgc
    23101 ttgcgccatg cggcgctggg tttctttgcg ggcagttaaa tcagagaaaa tcaccaaagc
    23161 acctatcatt tcaccgtgcg tgttatgaat acgactggtc gtgacactga gttcaatggt
    23221 gcggtcacgg cctggaaaac tgatctccag tgctacatgt tcggtgccat gttccagcgt
    23281 atccagtact ggactgtaga actgagtatt gtcgaacaac atggagtaag gctgccctac
    23341 cagttcatga cgttgatagc cagtgatgac ttctgctgct gggttcatgg tggttacatc
    23401 accctggcgg tcaatggcaa tgacgccatc ggcagcgttt tcaataatca gatcgttaag
    23461 tgttcgcgtt tcacgcagtg cctgggcgag gttgttaaca ctctgactga tttgccccat
    23521 ttcaccgggc aattgtggta atcgagtggg aatattttga gccagagtcg agaggccatc
    23581 ggtgatgata tctatattgg cgctcaggcg acgggagaaa aggacaatca acagcaggct
    23641 tatcagcaaa ccggcggtga gcacaatgat aatcctcaca tccattttcc aggcctggcg
    23701 gcgaatatct tcggttaatt cattggccca gatatagccg aggatttcac cattacgctc
    23761 aatgggaatc attgaattca aaatatcgcc gcgcacctgc ctgcctgaat aaaccaaagg
    23821 ggtatttgta cgcatgactt cgcgaccagg gtgatctgcg gcaatggtaa cgcccacatt
    23881 attctgatat agcgctgaag gcgcgtaggt gattatcgca tccagcgttt tgttgtaata
    23941 accagcgccg atgccaggga aggcgtgagt gatattttcg gtaatggggg caagttctgc
    24001 atttaatgcg cggatacgct cctcacgcgg taagtcgatg tagagatcgt agcgattgcc
    24061 tagcgcctga ttaagcaggt tgaccacggc agaaagtttt ttctcttttt cagataagac
    24121 tgctgaacgt ccttccgttt ctacgatata accaatagta agcgttggga caatgaccat
    24181 caggattgcc atcaggatca tttgattgcg taagcggcgt ggataaatcc acttcatata
    24241 atgcatgctg aaatccacta gtcttgtccg gtatatgacg attatcagag gttaaggtga
    24301 tgatttctcg gcggtgtatc atattccaga gaagagagaa cattgcggta acacgctttt
    24361 accgctacct taaccacact ccatcggtca cctgaggcgg agcttcgccc ctttgaaata
    24421 ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag cattggcgtt
    24481 agtgctctgg ctgttgattg ctttttcatc cgttttttac atcgttaatg cgttacatca
    24541 gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc gatcaggctc agcgctttat
    24601 tcaacgcacc tctgatgtga tgaaagagct gaagtacata gctgaaaatc gcttatcggc
    24661 agaaaacggt gtgctttccc cgcgtggacg agaaacgcag acggatgtgc ctgcgtttga
    24721 accgctattt gctgactctg attgttccgc aatgagtaac acctggcgag gttctctgga
    24781 gtcattggcg tggtttatgc gctactggcg cgataatttt tctgcggctt acgatctaaa
    24841 ccgggtattt ttaatcggca gcgataacct ctgcatggcc aatttcggtc tgcgtgatat
    24901 gccagtggaa cgcgataccg cgttaaaagc tttgcatgaa cgcattaata aatatcgaaa
    24961 tgcaccacaa gatgatagtg gcagtaacct ctactggatc agcgaaggtc cgcgccctgg
    25021 cgtcgggtat ttttacgcct tgacgccagt ttatctggcg aaccggttgc aggcgctttt
    25081 gggtgtcgag cagacaatcc ggatggagaa ctttttctta cctggtacgt tgccgatggg
    25141 ggttaccatt cttgatgaaa atggtcatac cctgatttcg cttaccggac cagaaagcaa
    25201 aattaagggc gatcctcgct ggatgcagga acgctcctgg tttggctata cggaagggtt
    25261 ccgggaactg gtgctgaaga aaaatctgcc accctcatcg ctaagcatcg tttattcagt
    25321 gccagttgat aaggtgctgg aacgcattcg catgttgatc cttaacgcaa ttttgctgaa
    25381 tgtgcttgcc ggagctgcat tgtttactct cgcgcggatg tacgagcggc gtattttcat
    25441 tccggcggaa agcgacgccc tgcgactgga agaacatgag cagtttaatc gcaagattgt
    25501 cgcctccgcg ccagtgggta tctgcatttt gcgtaccgct gatggcgtca atattttaag
    25561 taacgaactg gcgcatacct atctcaatat gcttacgcat gaggaccgcc aacggctgac
    25621 acaaattatc tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca ataataccaa
    25681 tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct
    25741 ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg caggagatgg cacaagcagc
    25801 ggaacaggcg agccagtcaa aatcgatgtt cctggccaca gtcagtcatg agctgcgaac
    25861 gccgctgtat ggcattatcg gtaacctgga tctgttgcaa accaaagagt taccgaaagg
    25921 cgtcgatcgt ctggtgacgg caatgaacaa ctcttccagc ctgttgttga aaattatcag
    25981 cgatattctc gatttctcga aaattgaatc agaacagttg aagatcgaac cgcgtgagtt
    26041 ttcaccgcgt gaagtgatga accacatcac cgccaactat ttaccgctgg tggtacgcaa
    26101 gcagttaggc ttgtactgct ttattgaacc ggatgtgcca gtggccttaa atggcgaccc
    26161 gatgcgttta caacaggtca tctctaacct gctgagtaac gccataaaat tcaccgatac
    26221 cggctgtata gttttgcatg ttcgcgctga tggcgattat ctctctatcc gtgttcgcga
    26281 taccggcgtg gggattccgg caaaagaagt ggtgcgtttg tttgatccct tcttccaggt
    26341 cggaacgggc gtacagcgta acttccaggg gactggtctg ggtctggcga tttgtgaaaa
    26401 actgatcagc atgatggacg gcgacatctc ggtagattca gaaccgggaa tgggcagcca
    26461 gtttaccgtg cgtattccgt tgtatggcgc tcagtacccg cagaaaaaag gtgtggaagg
    26521 gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga
    26581 aaccagtttg cagcgcagcg gcatcgtcgt tacaacatac gaagggcagg aaccgactcc
    26641 cgaagatgtg ttaattactg acgaggtagt gaacaaaaaa tggcagggca gagcggtagt
    26701 gaccttctgt cgtcgccata ttggtattcc gctggagaaa gcgccagggg agtgggtaca
    26761 cagtgtggct gctccgcatg agctaccggc attgttggcg cgtatttatt tgatcgagat
    26821 ggagagcgac gatcctgcta acgctctgcc gtcgacggac aaagcggtca gcgataatga
    26881 cgatatgatg attctggtcg tggatgatca tccgattaac cggcgtctgc tggcagatca
    26941 gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat ggcgtcgatg cgcttaatgt
    27001 acttaacaag aatcatattg atatcgtgct tagcgacgtc aatatgccaa atatggatgg
    27061 ttaccgcttg acgcaacgca ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac
    27121 tgctaatgcg ttggctgaag agaagcagcg gtgtctggag tccggtatgg acagttgcct
    27181 gtcgaagccg gtaacgctgg atgtgataaa acagacgctg acggtatatg ccgagagggt
    27241 caggaaatcg cgggaatcgt aggacggata aggcgtttac gccgcatctg gcattcagtg
    27301 caaatgccag atgcgacgct gacgcgtctt atctggccta caggtgatta gtctttatct
    27361 gccggactta acgtcacaga agagagataa ttcagcagag caatatcgtt ctcgacaccc
    27421 agcttcatca tcgcagattt cttctggcta ctgatggttt taatactgcg gttcagcttt
    27481 ttagcgatct cggtcaccag gaagccttcc gcaaacaggc gcagaacttc actctctttt
    27541 ggcgagagac gcttgtcacc ataaccacca gcactgattt tttccaacag gcgagaaacg
    27601 ctttccgggg taaatttctt ccctttctgc agcgcggcga gagctttcgg cagatcggtc
    27661 ggtgcacctt gtttcagcac gatcccttcg atatccagat ccaataccgc actaagaatc
    27721 gccgggttgt tgttcatggt cagaacaatg atcgacaggc ttgggaaatg gcgcttgatg
    27781 tacttgatta aggtaatgcc atcgccgtac ttatcgccag gcatggagag atcggtaatc
    27841 aacacatgtg catccagttt cggcaagttg ttgatcagtg ctgtagagtc ttcaaattcg
    27901 ccgacaacat tcacccactc aatttgctca agtgatttgc gaataccgaa caagactatc
    27961 ggatggtcat cggcaataat tacgttcata ttgttcatgt aataggctac cttgctacag
    28021 caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg gaacatcctt
    28081 ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca gatttagcat
    28141 ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt cactggttgc
    28201 tgcttcagta tatagcctct taacatcatc cggtactgtg tctacaaaga gcgcataata
    28261 gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg tcacctcttc
    28321 ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt
    28381 gaaattgacg cacaattgac caggcccaat ttcccgtacg ccagactcat catcgcttaa
    28441 aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat cataatcttg
    28501 actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta actggcgagt
    28561 gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat catccagtaa
    28621 tcgctcttct tcctcttcaa cttccgggtc agctgcgagc attttgatat gcacagagta
    28681 gcgtgtacca agcccatccc gcgttttgat gtttaaatga ccgcccagtt tacgcgccag
    28741 ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt tttgggtctg
    28801 gttgataaac gggaagtgca aattatccat ttcatgaatg cttacgcctt ctccagtgtc
    28861 cagaatgcgg aacgtcaggc ggtcttcgga ggactcatcc tgatcaacct caagggtgat
    28921 ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa
    28981 ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa tcagcagttg
    29041 cagaccttta cgcttgatgg caggcaacac tgaagggaca acttcatcaa ttaaatcctg
    29101 cacggagaac agctccgtct cacttttcca gctatcgtcc gcaagcatgt tcgctaactg
    29161 tatttcatcg accagccgca ccagcacatc tgcctgattc gccagttgtt tgccttccgg
    29221 ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg ggttctttca gcgcatcgcc
    29281 aatgtttttc ataaaggtca tccgcccctg ctggtttttc tcatacagac gctgcgcctg
    29341 cttgagtttc ttgtttacca gcacttcgcg atcctgatcg cgaataatga aaatttgtgt
    29401 gcgcggcgca acctggctgc gaaacatgcg gatctcatac agctcgttat tgatcgtcgc
    29461 ctgaataatc ccctgatgct gctccgccat ggtagtgatg ttttgcagat tcaaatgcgg
    29521 cagcaaatga tcggcaattt tgttacttat gaccgtgcgg tttgattcct gatcgtgaac
    29581 cagcaggccg agcggcagca gtgagactat ctcttcatta attgcccgta aaatgcgcaa
    29641 ttcgttattg accgccgtgc tggggacgct ttctgtactg cggctggaga aatggcggaa
    29701 tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca gtggcagcag
    29761 aatgttttgc aacgtatcca gcagtaaggt gccgtaagga acctgccaga ccaggcgcat
    29821 atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt ggatactgac
    29881 gctatccgtc ccttctttct cattttcatt gtttcccgtt gccgtcgcgt ctggctcaag
    29941 gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggca gatcaaaagc
    30001 cacgaccgtt gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa agtaatgacc
    30061 gttctgccag gccaggcggc gcatgttaga aaaactttcg cgttcatcga gggcgttggc
    30121 ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg ctgacggtcg attctttaaa
    30181 tccggaggtg agatctttga ggggcagggt tgagatcagc accagactgt tatcctgacc
    30241 attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtgt ccagataggt
    30301 ggacatccgt tgagtcatct caagcgttga gttgtcgtga gagccaaaga ttaaggcttc
    30361 cgttttgcgg cgcggttttt ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa
    30421 accttcgccg gaggaggggg tggtcgtcgc ggcaatgttg tcgtagatct gccaggtcac
    30481 gtaacgccag gtatcgacgc gcttttggat agcgtgggta atgtcgacaa tctggtaact
    30541 tttatctttc agccaggcgt taacggcgct ttgtaccatt acacccatcg tcaccagtaa
    30601 cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt
    30661 cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt
    30721 gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat tgatgattct
    30781 ctattctcca gtgatattta tcatcctgca ttgggggaaa tagatgtaca agcgccattt
    30841 tgctgtacaa ataacctaca aaaagcctaa cgaaattaat tgcaaataaa cgaagattgc
    30901 tggaaattat gctgatgtta attatttgtg aaatagttaa caagcgttat agtttttctg
    30961 tggtagcaca gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccgg atgcgaggca
    31021 tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta
    31081 atgatgatag cggaagatat tctagttgcg agtgaaggtt ttgttttgac attcagtgct
    31141 gtcaaataat taagaataag ttattgattt taaccttgaa ttaatattgc ttgatgttag
    31201 gtgcttattt ggccattctg caataatctt aaaaagttcc cttacattta cattctgaaa
    31261 catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat
    31321 tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag
    31381 tggcataaaa aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta
    31441 ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac
    31501 aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct
    31561 gacgacaagt ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact
    31621 caggttactg accaactgac cggttacggc cagtgggaat atcagatcca gggcaacagc
    31681 gctgaaaacg aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat
    31741 gtgggttctt tcgactacgg tcgtaactac ggcgttgttt atgacgtaac ttcctggacc
    31801 gacgtcctgc cagaattcgg tggcgacact tacggttctg acaacttcat gcagcagcgt
    31861 ggtaacggct tcgcgaccta ccgtaacacc gacttcttcg gtctggttga cggcctgaac
    31921 tttgctgttc agtaccaggg taaaaacggt agcgtagatg gcgaaggcat gaccaacaac
    31981 ggtcgtggtg ctctgcgtca gaacggcgac ggcgttggcg gttctatcac ttatgattac
    32041 gaaggcttcg gtatcggtgc tgcagtgtcc agctccaaac gtactgacgc tcagaatggt
    32101 acttacgtag ttgataatgt aactcataac tacatcggta ctggcgaccg tgctgaaacc
    32161 tacactggtg gtctgaaata cgacgctaac aacatctacc tggctgctca gtacacccag
    32221 acctacaacg caactcgcgt aggttccctg ggttgggcga acaaagcaca gaacttcgaa
    32281 gctgttgctc agtaccagtt cgacttcggt ctgcgtccgt ccctggctta cctgcagtct
    32341 aaaggtaaaa acctgggtgt catcaatggc cgtaactacg acgacgaaga tatcctgaaa
    32401 tatgttgatg ttggtgcgac ctactacttc aacaaaaaca tgtccaccta cgttgactac
    32461 aaaatcaacc tgctggacga caaccagttc actcgtgacg ctggcatcaa cactgataac
    32521 atcgtagctc tgggcctggt ttaccagttc taatctcgat tgatatcgaa caaagggcct
    32581 gcgggccctt ttttcattgt tttcagcgta caaacacagt tttttggtgt actcttgcga
    32641 ccgttcgcat gaggataatc acgtatggaa ataagcttta cccgcgtggc actgctggct
    32701 gccgcgctct tctttgttgg ttgcgatcaa aaaccacaac ccgccaaaac ccacgctact
    32761 gaagttaccg ttcttgaagg caaaaccatg ggtaccttct ggcgtgccag catcccgggc
    32821 attgacgcca aacgcagtgc cgaacttaaa gaaaagattc agacccaact ggacgctgac
    32881 gatcagctgc tttcgaccta taaaaaagat tccgcgctga tgcgctttaa cgactcgcaa
    32941 agtttgtcgc cgtggccggt aagtgaagcg atggccgata tcgtcaccac ctcgctgcgt
    33001 attggcgcaa ggaccgatgg tgcgatggat ataaccatcg ggccgctggt gaatctgtgg
    33061 ggctttggtc cggaacaaca accggttcaa attccgagcc aggaacagat cgatgcgatg
    33121 aaagccaaaa ccggcttaca gcacctgacg gtcattaatc agtcgcatca gcaatatctg
    33181 caaaaagacc tgccggattt atatgtcgat ctctccaccg tcggcgaagg ttatgcggcg
    33241 gatcacctgg cacgcttgat ggagcaggaa gggatttccc gctatctggt gtcggtgggc
    33301 ggcgcgctga acagccgtgg tatgaacggt gctggcaagc cgtggcgggt ggcgattcaa
    33361 aaaccaaccg ataaagaaaa cgcggttcag gccgtggtgg atatcaacgg tcatgggatt
    33421 agtacctctg gcagctaccg taactattac gaactggacg gcaaacgtct ttctcatgtt
    33481 atcgatccgc aaaccgggcg tcctatcgaa cacaatctcg tatccgtgac ggtgattgcc
    33541 ccgacggcgc tggaagccga tgcctgggat actggcttaa tggtactcgg accggagaaa
    33601 gccaaagagg ttgttcgccg ggaaggactg gcggtctaca tgatcaccaa agagggcgat
    33661 aattttaaaa cctggatgtc gccgcagttt aaaagcttcc ttatcagcga aaaaaattaa
    33721 agcgcaagat tgttggtttt tgcgcgatgg tgaccgggca gactaacggc tatccttaac
    33781 cagggagctg attatgaaaa acgccacatg tttaactgac gatcaacgct ggcaatctgt
    33841 cttagcccgc gacccgaacg ccgacggcga attcgttttc gccgtgcgca ccacgggtat
    33901 cttttgccgt ccgtcttgcc gagccagaca tgccttgcgg gaaaatgtct ccttctacgc
    33961 aaatgccagc gaggcgcttg ccgctggctt tcgtccctgc aaacgttgtc agccagacaa
    34021 agccaatccc cggcaacatc gccttgataa aattacccac gcctgccgac ttctggaaca
    34081 ggaaacgcct gtaacgctgg aagccttagc cgaccaggtg gcgatgagtc catttcattt
    34141 gcatcggttg tttaaagcga ctaccggaat gacgcctaaa gcctggcaac aggcctggcg
    34201 cgctcgccgt ttgcgcgaat tgctggcgaa aggggagagc gtgactacat ctattcttaa
    34261 cgccggattc cccgacagca gcagttacta ccgcaaagcc gacgaaacgc tgggcatgac
    34321 ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg
    34381 tgagctgggc cgttgcctgg tggcagaaag tgagcggggg atttgcgcga tattactggg
    34441 cgatgatgac gcgacactaa tcagcgagtt acagcagatg tttcccgctg ccgacagcgc
    34501 gcctgccgat cttacgtttc agcaacatgt gcgtgaagtg atcgccagcc tcaatcaacg
    34561 cgatacgccg ctgacgttac cactggacat tcgcggcact gcttttcagc aacaagtctg
    34621 gcaggcactg cgcacgatac cttgcggtga aaccgtcagt tatcagcaac tggctaacgc
    34681 cattggcaaa ccgaaagcgg tacgggccgt tgccagcgcc tgtgccgcca acaagctagc
    34741 tatcgttata ccttgtcatc gggtggtccg tggtgatggc acactttccg gttaccgctg
    34801 gggcgtgtcg cgcaaagcgc aactgctgcg ccgcgaagct gaaaatgagg aaaggtaatg
    34861 ttggatctgt ttgccgatgc tgaaccgtgg caagaaccac tggcggctgg agcggtgatt
    34921 ttgcaccgtt ttgcttttaa cgctgcggag caactgatcc gcgatattaa tgacgttgcc
    34981 agccagtcgc cgtttcgcca gatggtcact ccaggcggat ataccatgtc ggtggcgatg
    35041 accaactgtg ggcatctggg ctggacgacc catcggcaag gttatctcta ttcgcccatt
    35101 gatccgcaaa caaataaacc gtggcccgcc atgccgcaga gttttcatca tttatgtcaa
    35161 cgtgcggcta cggcggcggg ctatccagat ttccagccag atgcctgttt gatcaaccgc
    35221 tacgttcctg gcgcgaaact gtcgctgcat caggataaag acgaaccgga tctgcgcgcg
    35281 ccaattgttt ctgtttctct gggcttaccc gcgatttttc aatttggcgg cctgaaacga
    35341 aatgatccgc tcaaacgttt gttgttggaa catggcgatg tggtggtatg ggggggtgag
    35401 tcgcggctgt tttatcatgg cattcaaccg ttgaaagtgg gggttcatcc actcaccacc
    35461 gactgccgct acaacctgac attccgtcag gcaggtaaaa aagaataaaa ataagaatta
    35521 ttattgctgt gcgcacgaag atgtttaaac tgcgggctgt aattcattgt ccgggttttc
    35581 tgcatggaac ttcttgtact tgtctggcgg cagtatcgct ggccatttat cagtgtgatg
    35641 gcgctaagcc tcgccagtgc ggcattaggc attggcttaa ttgcttttat caatcagcgc
    35701 cttatcgaaa cggcggatac cagtctgctg gtattgccgg agtttctggg gttattgctg
    35761 ctgttgatgg ccgtcactct cggatcgcaa ctggcgctca ccactttggg gcatcacttc
    35821 gtttaccgac tgcgtagtga atttatcaag cggattctcg atactcacgt cgagcgcatt
    35881 gaacaactcg gtagcgcctc gttgctggcg ggattaacca gcgatgtgcg caatatcacc
    35941 attgcttttg tgcgtctgcc ggaactggtg caggggatca ttctcactat cggttcagcg
    36001 gcgtatttgt ggatgctgtc gggcaaaatg ttgctggtaa cggctatctg gatggcagtc
    36061 accatctggg gcggttttgt gctggtggcg cgggtgtaca aacatatggc gaccctgcgt
    36121 gaaaccgaag ataagctgta cacggatttt caaactgtac tcgaagggcg caaagagctg
    36181 actctgaacc gtgaacgcgc cgagtatgtg tttaacaacc tctacattcc tgatgcgcaa
    36241 gagtatcgcc atcatattat tcgcgcagac accttccatc ttagtgccgt gaactggtca
    36301 aacatcatga tgctgggcgc tatcggcctg gtgttctgga tggcgaacag tctcggttgg
    36361 gctgatacca acgttgctgc gacctattcg ttgacgctgt tattcctgcg tacgccgctg
    36421 ctttcggcgg ttggcgcatt gccgacgctg ctgacggcgc aggtggcgtt taacaagctg
    36481 aacaaattcg cgctcgcgcc tttcaaagca gagtttccgc gcccgcaggc ttttcccaac
    36541 tggcaaacgc tggagctgcg taacgtgacg tttgcttatc aggataacgc gttttccgtt
    36601 gggccgatta acctcaccat caaacgtggt gaactgctgt ttctgattgg tggcaacggt
    36661 agtggaaaat cgacgctggc gatgttgctg acgggcttgt accaaccaca aagcggcgaa
    36721 atcttgctgg atggcaaacc tgtcagcggc gaacaaccgg aagattatcg caaactgttt
    36781 tcggcagtgt ttaccgatgt ctggctgttt gatcaactgc tggggccgga ggggcaaccc
    36841 gctaacccgc aactggttga gaagtggctg gcgcagctga aaatggctca taagcttgag
    36901 ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg
    36961 ctgttgctgg cgctggcaga agaacgcgat attatcctgc tggatgaatg ggcggcggat
    37021 caggatccac acttccgtcg tgagttttat caggtgctgc tgccgctgat gcaggagatg
    37081 ggtaaaacta ttttcgccat cagtcatgat gatcattact ttatccacgc cgaccgcctg
    37141 ctggaaatgc gcaacgggca acttagcgag ctgacaggcg aagagcgcga tgctgcttcg
    37201 cgtgatgccg ttgcccggac ggcataatat tacgacgctg acctggcgga ctgctgccgc
    37261 c
//
LOCUS       DADTFG010000027        36447 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.027, whole genome shotgun sequence.
ACCESSION   DADTFG010000027 DADTFG010000000
VERSION     DADTFG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.027"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            352..1722
                     /gene="aroP"
                     /locus_tag="J1138_001314"
     CDS             352..1722
                     /gene="aroP"
                     /locus_tag="J1138_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBA8453136.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHDFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTSFPALLYPLGNWVCLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1765..2619)
                     /gene="ampE"
                     /locus_tag="J1138_001315"
     CDS             complement(1765..2619)
                     /gene="ampE"
                     /locus_tag="J1138_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBA8453137.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     CMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDKREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2616..3167)
                     /gene="ampD"
                     /locus_tag="J1138_001316"
     CDS             complement(2616..3167)
                     /gene="ampD"
                     /locus_tag="J1138_001316"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBA8453138.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3255..4148
                     /gene="nadC"
                     /locus_tag="J1138_001317"
     CDS             3255..4148
                     /gene="nadC"
                     /locus_tag="J1138_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBA8453139.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4352..4792
                     /gene="ppdD"
                     /locus_tag="J1138_001318"
     CDS             4352..4792
                     /gene="ppdD"
                     /locus_tag="J1138_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBA8453140.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4802..6187
                     /gene="gspE"
                     /locus_tag="J1138_001319"
     CDS             4802..6187
                     /gene="gspE"
                     /locus_tag="J1138_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBA8453141.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQMLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6177..7379
                     /gene="hofC"
                     /locus_tag="J1138_001320"
     CDS             6177..7379
                     /gene="hofC"
                     /locus_tag="J1138_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBA8453142.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQYPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLIDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7414..8457)
                     /gene="guaC"
                     /locus_tag="J1138_001321"
     CDS             complement(7414..8457)
                     /gene="guaC"
                     /locus_tag="J1138_001321"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBA8453143.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            8682..9302
                     /gene="coaE"
                     /locus_tag="J1138_001322"
     CDS             8682..9302
                     /gene="coaE"
                     /locus_tag="J1138_001322"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBA8453144.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQR
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9302..10045
                     /gene="zapD"
                     /locus_tag="J1138_001323"
     CDS             9302..10045
                     /gene="zapD"
                     /locus_tag="J1138_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBA8453145.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQLETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10055..10252
                     /gene="yacG"
                     /locus_tag="J1138_001324"
     CDS             10055..10252
                     /gene="yacG"
                     /locus_tag="J1138_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBA8453146.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10456..10854)
                     /gene="mutT"
                     /locus_tag="J1138_001325"
     CDS             complement(10456..10854)
                     /gene="mutT"
                     /locus_tag="J1138_001325"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBA8453147.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKVEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10914..13619)
                     /gene="secA"
                     /locus_tag="J1138_001326"
     CDS             complement(10914..13619)
                     /gene="secA"
                     /locus_tag="J1138_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBA8453148.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13681..14193)
                     /gene="secM"
                     /locus_tag="J1138_001327"
     CDS             complement(13681..14193)
                     /gene="secM"
                     /locus_tag="J1138_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBA8453149.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14424..15341)
                     /gene="lpxC"
                     /locus_tag="J1138_001328"
     CDS             complement(14424..15341)
                     /gene="lpxC"
                     /locus_tag="J1138_001328"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBA8453150.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15442..16593)
                     /gene="ftsZ"
                     /locus_tag="J1138_001329"
     CDS             complement(15442..16593)
                     /gene="ftsZ"
                     /locus_tag="J1138_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBA8453151.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16654..17916)
                     /gene="ftsA"
                     /locus_tag="J1138_001330"
     CDS             complement(16654..17916)
                     /gene="ftsA"
                     /locus_tag="J1138_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBA8453152.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17913..18743)
                     /gene="ftsQ"
                     /locus_tag="J1138_001331"
     CDS             complement(17913..18743)
                     /gene="ftsQ"
                     /locus_tag="J1138_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBA8453153.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18745..19665)
                     /locus_tag="J1138_001332"
     CDS             complement(18745..19665)
                     /locus_tag="J1138_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBA8453154.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19658..21133)
                     /gene="murC"
                     /locus_tag="J1138_001333"
     CDS             complement(19658..21133)
                     /gene="murC"
                     /locus_tag="J1138_001333"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBA8453155.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21187..22254)
                     /gene="murG"
                     /locus_tag="J1138_001334"
     CDS             complement(21187..22254)
                     /gene="murG"
                     /locus_tag="J1138_001334"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBA8453156.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSREILLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22251..23495)
                     /gene="ftsW"
                     /locus_tag="J1138_001335"
     CDS             complement(22251..23495)
                     /gene="ftsW"
                     /locus_tag="J1138_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBA8453157.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23495..24811)
                     /gene="murD"
                     /locus_tag="J1138_001336"
     CDS             complement(23495..24811)
                     /gene="murD"
                     /locus_tag="J1138_001336"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBA8453158.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24814..25896)
                     /gene="mraY"
                     /locus_tag="J1138_001337"
     CDS             complement(24814..25896)
                     /gene="mraY"
                     /locus_tag="J1138_001337"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBA8453159.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25890..27248)
                     /gene="murF"
                     /locus_tag="J1138_001338"
     CDS             complement(25890..27248)
                     /gene="murF"
                     /locus_tag="J1138_001338"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBA8453160.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27245..28732)
                     /gene="murE"
                     /locus_tag="J1138_001339"
     CDS             complement(27245..28732)
                     /gene="murE"
                     /locus_tag="J1138_001339"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBA8453161.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28719..30485)
                     /locus_tag="J1138_001340"
     CDS             complement(28719..30485)
                     /locus_tag="J1138_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBA8453162.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30501..30866)
                     /gene="ftsL"
                     /locus_tag="J1138_001341"
     CDS             complement(30501..30866)
                     /gene="ftsL"
                     /locus_tag="J1138_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBA8453163.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30863..31804)
                     /gene="rsmH"
                     /locus_tag="J1138_001342"
     CDS             complement(30863..31804)
                     /gene="rsmH"
                     /locus_tag="J1138_001342"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBA8453164.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31806..32264)
                     /gene="mraZ"
                     /locus_tag="J1138_001343"
     CDS             complement(31806..32264)
                     /gene="mraZ"
                     /locus_tag="J1138_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBA8453165.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32866..33870)
                     /gene="cra"
                     /locus_tag="J1138_001344"
     CDS             complement(32866..33870)
                     /gene="cra"
                     /locus_tag="J1138_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBA8453166.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33952..34038)
                     /locus_tag="J1138_001345"
     CDS             complement(33952..34038)
                     /locus_tag="J1138_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBA8453167.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34050..34541)
                     /gene="ilvN"
                     /locus_tag="J1138_001346"
     CDS             complement(34050..34541)
                     /gene="ilvN"
                     /locus_tag="J1138_001346"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBA8453168.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34544..36268)
                     /gene="ilvI"
                     /locus_tag="J1138_001347"
     CDS             complement(34544..36268)
                     /gene="ilvI"
                     /locus_tag="J1138_001347"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBA8453169.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEVLNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARLVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTHGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
BASE COUNT         8778 a        10191 c         8918 g         8560 t
ORIGIN      
        1 tattcttata tgaccttttg taagattatt gtgctggtgg ctaaggcatg gtgttcagca
       61 ggcagacaaa tccgacatat agctagttaa aacagtatgt tatcagactt aaagtatcta
      121 ttaaatgtga tgaaaaactt acaattgctt tttttttatc cagaaaatca acacatctac
      181 ataaagcagg tgcattctcg cgcatatacc attatcttgt gatagttgaa agtactatca
      241 tcataattca gttacataaa atgtaaagaa accaatacaa caaacggaat tacaaaatta
      301 cacacgcatt gctgcgtagt tcaaaaaaac aacacaacac gaggtttcat aatggaaggt
      361 caacagcacg gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg
      421 ctgggtggcg cgatagggac agggctattc ctgggtagcg cctccgtaat acagtccgca
      481 gggccaggga ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt
      541 cagctaggtg aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat
      601 aaatactggg gcagttttgc cggtttcgcc tctggctgga actactgggt actgtacgtt
      661 ttagttgcca tggcagagct gactgccgtg ggtaaataca ttcagttctg gtatccggaa
      721 atcccaacct gggtttctgc cgccgtgttc tttgtggtga tcaacgccat caacctgaca
      781 aacgtaaaag tgtttggcga gatggagttc tggtttgcca ttatcaaagt tatcgcggtg
      841 gtagcgatga tcatcttcgg cgcctggctg ctattcagcg gcaacggcgg cccgcaggcg
      901 agcgttagca acctgtggga tcagggtggt ttcctgccac acgacttcac cggtctggtg
      961 atgatgatgg cgattatcat gttctctttc ggtggtctgg aactggtggg gatcaccgca
     1021 gcagaagctg ataacccgga gcaaagtatc ccgaaagcaa ctaaccaggt tatctaccgc
     1081 atcctgattt tctatattgg ctcgttagcc gttctgctct cactaatgcc gtggacccgc
     1141 gttaccgccg ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgta
     1201 gcgaatgcgc tgaatatcgt ggtactgact gctgcgctct ccgtgtacaa cagctgcgta
     1261 tattgcaaca gccgtatgct gttcggtctg gcacaacaag ggaacgcgcc aaaagcgctg
     1321 gcgtctgtcg ataaacgcgg cgtaccggtt aacactattc tggtgtctgc gctggttacg
     1381 gcattgtgcg tactgattaa ctaccttgcc ccagagtccg ctttcggact gttaatggcg
     1441 ctggtggtat ctgcactggt catcaactgg gcgatgatca gtctggcgca tatgaaattc
     1501 cgtcgagcca aacaagaaca aggcgtggta actagcttcc ctgccctgct ttatccgctg
     1561 ggtaactggg tctgcctgct gtttatggcg gcggtactgg tgattatgct gatgacccca
     1621 ggaatggcga tttcggtata cctgatcccg gtatggctga tcgtgttagg tatcggctat
     1681 ctgtttaaag agaaaaccgc caaagccgta aaagcgcatt aatctctcta cgccctcacc
     1741 cgtgcagggt gagggcaata atctttacac caacgccccg taaatcgtca gtagcgcaat
     1801 aaccaccacg accacgaacg aggttttctt cgccattgaa accgctgcct ttggcgtctc
     1861 caccttatcg acatgcggtt cacgcgccag agagaactgt gccagacgcg ttaatacctg
     1921 atattgcgaa gtatggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc
     1981 atgaccgatc aaggcatata ccacacccgc aagacgaacc ggcacccaat ccagtacatg
     2041 aagcacggca tcaatgccgg actgtaaacg atgatgcggc gtctggtatc gcgccagcca
     2101 gtattgccat gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc
     2161 cacaatcagc cagaacagcg gagcaagata aaaacgaaag ttaatccaca gcaatgcatt
     2221 ttgcagctca cgcaaatact cacgtttatc gcagcctgcc gggacgccgt gaatcatggt
     2281 gagttcgcca gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata
     2341 agcatgataa tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca
     2401 caccagcagc gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt
     2461 cacgcccatc gcaataatgg tcatgcataa cgtgcgcccg agagaaaaat gtttcacccg
     2521 ccgaaagaac gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg
     2581 ctcgaaaatt aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc
     2641 gcacgaaacc gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata
     2701 tcacaatggc cagtcatgtt attagcgata tccggatagc gatcaatcag tgcccgcgta
     2761 accgccgcaa gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttccagc
     2821 tcaatcccaa tagaaaaatc attgcagcgt tcacgcccct gatactgaga gactcccgca
     2881 tgccaggcac gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa
     2941 cagtgagcgg agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca
     3001 atagttccag tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta
     3061 atattgtgca ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt
     3121 gagggaacgc ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt
     3181 atatgtggtg ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta
     3241 tctggagttt taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc
     3301 tggaacgcat taatctcgat atccccggcg cggtggccca ggcgctacgg gaagatttag
     3361 gcggaacagt cgatgccaac aatgacatta cggcaaaact tttaccggaa aactctcgct
     3421 ctcatgccac agtgatcacc cgcgagaatg gcgtattttg cggcaaacgc tgggttgaag
     3481 aggtgtttat tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg
     3541 atgtcatcaa cgccaatcaa cccttgttcg aacttgaagg cccatctcgc gtgctgttaa
     3601 cgggcgaacg cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac
     3661 gccactatgt cgaattactg gaaggcacca acacgcagct gttggatacg cgcaaaacct
     3721 tacccggcct gcgttcagct ctgaaatacg cggtactttg cggcggcgga gcgaatcacc
     3781 gtctggggct ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag
     3841 tgcgccaggc ggtcgaaaaa gcgtcctggt tgcacccgga tgcgccagta gaagtcgaag
     3901 tagagaatct ggaagaactt gatgaagccc tgaaagcagg tgccgatatc atcatgctgg
     3961 ataacttcga aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac
     4021 tggaagtgtc tggcaacgtc actgacaaaa cactgcgcga atttgccgaa acgggcgtgg
     4081 actttatctc cgttggtgcg ctaactaaac acgtacaggc actcgacctt tcaatgcgtt
     4141 ttcgctaatt gctaacagat ttatccgctc catcgcgggc ggataaatct ccccttcccc
     4201 aactttcttc gtaacacctc gcaaatttct tgtctttcgc tgcaacgaag taaaaaacct
     4261 cggaagctgc tttcccgttt cgttttttgc ccctcgcttg tgctgatggc gtttgccaaa
     4321 gtagcgccaa ccaaatcaag gagcgaaaca gatggacaag caacgcggtt ttacacttat
     4381 cgaactgatg gtggttattg gcatcattgc cattttaagc gccattggta ttcccgctta
     4441 tcaaaactac ctgcgcaaag ccgcactcac cgacatgcta caaacctttg tgccttaccg
     4501 taccgccgta gagttgtgcg cgctggaaca tggtggatta aatacctgcg acggtggcag
     4561 caatggcatt ccctcgccta ccaccacccg ctatgtttca gccatgagtg tggcaaaggg
     4621 cgtggtgtcg ctgaccgggc aagaaagtct caatggacta agcgtcgtca tgacgccggg
     4681 ttgggataac gcaaacggcg tcaccggctg gacgcgtaac tgcaatattc aaagtgacag
     4741 cgcattgcag caagcctgcg aagatgtctt ccgctttgat gacgccaact aaggagcggc
     4801 aatgaatatt ccacagctca ctgccctgtg tctgcgttat cagggagtct tgctggatgc
     4861 cagcgaagag gtggttcatg ttgcggtagt cgatgcacct tcgcatgagc tactggacgc
     4921 attgcatttc gctaccacca aacgtattga gatcacctgc tggacacgcc aacaaatgga
     4981 aggtcacgcc agtcgcacac aacagacatt gcccgtagct gttcaggaga agcatcagcc
     5041 caaagcagag ttgctgactc gaacgttaca atctgcgcta gaacaacgcg cgtctgatat
     5101 tcatatcgaa ccagcggaca atgcctaccg catccgcttg cgtatcgacg gcgtattgca
     5161 tcctttaccg gacgtttcac cggatgccgg agtcgcatta accgccagat taaaagtgct
     5221 gggaaacctg gatattgcgg aacatcgcct gccgcaggac gggcaattca ctgtcgaact
     5281 ggcaggaaac gccgtctcat ttcgtattgc gaccttagca tgtcggggtg gtgaaaaggt
     5341 ggtattaagg ttgttacagc aggtgaacca ggcactggat gttaacacgc ttggaatgca
     5401 gccgtcacaa ctggtggact ttgctcatgc cttgcaacaa ccacagggac tggtgctggt
     5461 aactggccct acaggcagcg gcaaaacggt cacgctttat agtgccctgc aaatgctgaa
     5521 taccgctgac attaatattt gtagcgtcga agatccggtt gagatcccca tagccggact
     5581 aaaccagacg caaatccatt cgcgtgccgg actcaccttt cagggcgttt tgcgtgcgtt
     5641 attgcgccag gatcctgacg tcataatgat cggagagatc cgcgatggcg aaacggcaga
     5701 aattgccatt aaagccgcgc aaaccggtca cctggtgttg tctaccctac acactaattc
     5761 cacctgcgaa acgctggtac gtttacagca aatgggagtc gcccgctgga tgctatcatc
     5821 ggcgcttacg ctggtaatag cccagcgtct ggtacgcaaa ctttgcccgc attgtcgccg
     5881 gcagcaaggg gagcccatcc atattccaga caatgtatgg ccgtcgccgc tgccccactg
     5941 gcaggcaccc ggttgtgtac attgctacca cggtttttat ggtcgcacgg ccttatttga
     6001 agttctgccc ataacaccgg tcatacgtca gcttatttcc gctaataccg acgttgaatc
     6061 gctggaaacg cacgcccgac aggcgggtat gcgaacgctt tttgaaaacg gctgcctggc
     6121 cgtggagcaa ggcttaacca cctttgaaga gttaatccgc gtattgggga tgccgcatgg
     6181 cgagtaagca actctggcgc tggcatggca taaccggcga cggcaatgcg caagatggga
     6241 tgctatgggc agagagccgt gctttgctgc tcatggcact acagcaacag atggttaccc
     6301 cactaagcct gaagcgaatc gccatcaatt ctgcgcagtg gcgaggagat aaaagcgcgg
     6361 aagtcattca tcaactggcg acgctactca aagccgggtt aacgctttct gaagggctgg
     6421 ctctgctggc ggaacagtat cccagtaagc agtggcaagc gttgctgcaa tcactggcgc
     6481 acgatcttga acagggcgtt gccttttcca atgccttatt accctggtca gaggtatttc
     6541 cgccgctcta tcaggcgatg atccgcacgg gtgaactgac cggtaagctg gatgaatgct
     6601 gctttgaact ggcgcgtcag caaaaagccc agcgtcagtt gatcgacaaa gtgaaatcag
     6661 cgttacgtta tcccattatc attttagcga tggcaatcat ggtggttgtg gcaatgctgc
     6721 attttgtact gccggagttt gccgctatct ataagacctt taacactccg ctaccggcgc
     6781 taacgcaggg gatcatgacg ctggcaagtt ttagtggcga atggggttgg ttactggtgc
     6841 tattcggctt tctgctggcg atagccaata agctgctgat gcgccaccca accaggctta
     6901 tcgcacggca gaaattgctg ttacgcatcc cgattatggg ttcactgatg cggggacaaa
     6961 aactcacgca gatctttacg attctgacac tgacacaaag tgcaggcatt tcttttttac
     7021 agggcgttga gagcgtcaga gaaacaatgc gctgcccgta ctgggtgcaa cttctgacac
     7081 aaatccagca cgatatcagt aacggtcacc ccatctggct ggcgctaaaa aatgccggtg
     7141 agtttagccc gctctgtttg caattagtga gaacaggaga ggcatccggc tcgctggatc
     7201 tcatgttaga caacctcgcc catcatcatc gggataacac aatggcgctg gcggataacc
     7261 tcgcagcctt actggaaccg gcgttgctga tcataacggg aggaattatc ggtacgctgg
     7321 tggtggcaat gtatctgcca attttccatt taggcgatgc gatgagtgga atgggataac
     7381 gctggcgtgt tgctccacgc cagcgttggt atattacagg ttgttgaaga tgcggttttc
     7441 ttgttcctgc acacggataa acgtggtgcg cttggtcagt tctttcaggc gtgaagcccc
     7501 aacgtatgta caagctgaac gcaggccgcc caaaatatca cgcgcggtat tttcaaccgg
     7561 gcctcgcagc ggcagcttaa cggttttacc ttctgctgcg cgatattccg caacgccgcc
     7621 aacgtgacgt ttcatcgcag actctgagct catgccgtag aacaacataa atttctcgcc
     7681 gttctcctca acgatgcgac cgccgctctc ttcgtggccc gccagcatgc cgccaagcat
     7741 tacgaaatcg gcaccgccgc caaaggcttt cgcgacatcg cccggcgtgg tgcagccgcc
     7801 atcgctgacg atcattccgc ccagaccgtg cgcagcatcg gcgcattcga tcaccgcaga
     7861 aagttgcgga taaccgacgc ctgttttgac gcgagttgta caaacagaac ctgggccaat
     7921 gccaacttta acgatatcgg caccggagag gataagctcc tcacacattt caccagtcac
     7981 tacgttgcct gcacaaatgg ttttggtcgg ccacgcttca cgcgctttcg caacgaactg
     8041 cacgaagtgt tcggaataac cattcgccac gtcaatacaa acgaaattta atgccgggtt
     8101 caggtcgaga atctgtttag ttttttcgaa atccgcatca gacgtgccgg tagaaaccat
     8161 cacatgtttc agcacatcag cggaagaatt gttgataaac gcttgccact cttcgacaga
     8221 atagtgttta tgcacagcag tcagaatatc aaaagaagcc agcgcagagg ccatagaaaa
     8281 tgtgcctacg gtgtccatat tggcggcgat aatcggcacg ccggaccagc tctgacctga
     8341 atgtttgaag gtgaattgac gttccagttc aacatcggaa cggcttttaa gagtggagcg
     8401 tttagggcgg atgagaacgt ctttaaaacc taacttcaga tcttcttcaa tacgcatgtg
     8461 cggattcctg gggttaatgg ctataagggt aagagcgcaa ctccagtgac gttatcatac
     8521 gcactaatca atgcggcgca agactgcgaa attgtctttt tttacgctac aatcccataa
     8581 atttacctgg taaatagtga gcgaacgttt catcaatttc gctttcgtgc gctcattttt
     8641 aacgttttga taatcatgat gaattcccgg gaatataaat tatgaggtat atagttgcct
     8701 taacgggagg cattggcagt ggcaagagta ccgttgccaa tgcgtttgct gatctcggaa
     8761 ttaacgtcat tgatgccgat attattgcgc gtcaggtggt tgaaccaggt gcacctgcgc
     8821 tacatgccat tgctgatcac tttggcgcta acatgattgc tgctgatgga acattgcagc
     8881 gccgggcctt gcgcgagcgg atcttcgcca acccggaaga gaaaaactgg cttaacgccc
     8941 tgctgcatcc gctgattcag caagagacgc aacaccagat ccagcgagct acttccccct
     9001 atgtactatg ggttgtgcca ttgctggtag aaaactcact gtataaaaaa gcgaatcgag
     9061 tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg caccatgcag cgcgacgatg
     9121 taactcgcga gcatgtcgaa caaatccttg ctgctcaggc aacgcgcgaa gctcgccttg
     9181 ccgtggcaga tgacgtcatt gataataacg gcgcaccgga tgctatcgca tcggatgttg
     9241 cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt tgtctcacag gaaaaaccgt
     9301 aatgcagacc caggtccttt ttgaacatcc actaaatgaa aaaatgcgta catggctgcg
     9361 cattgagttt ttgattcagc aactcaccgt taatttaccc atcgttgacc acgctggcgc
     9421 gctgcatttc ttccgtaatg tcagcgaatt actggatgtt ttcgagcgcg gcgaagtccg
     9481 cactgagctg ttgaaagaac ttgaccggca gcaacgtaaa ctccagacct ggattggcgt
     9541 gcccggcgtg gaccagagcc gtattgaggc attaattcag cagttaaaag cggcggggag
     9601 cgtattaatc tccgcgccgc gtatcgggca atttctacgt gaagatcgtt tgattgctct
     9661 ggtgcgtcag cgactgagca tcccgggcgg ctgttgcagc tttgatttac ctacattgca
     9721 catttggctg catctacccc aggcgcagcg cgacagccag ctagaaacct ggattgccag
     9781 cctgaacccg cttacccagg cacttaccat ggtgctggat ttaattcgcc agtcggcccc
     9841 cttccgtaaa caaaccagcc tgaatggttt ttatcaggat aacggtggcg atgccgactt
     9901 gctgcgcctg aatctgtcgc tcgattcaca gctttatccg caaatttccg gtcataagag
     9961 ccgttttgcc attcgtttta tgccgctgga cagtgaaaac ggacaggtac cagaacgtct
    10021 ggatttcgaa ctggcctgtt gctaaggagt aaaaatgtca gaaactatta cggtgaattg
    10081 cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc ccgtttcggc cattttgctc
    10141 caaacgttgt cagctgattg acctcggaga atgggctgct gaagaaaaac gaatccccag
    10201 cagtggcgat ctttccgaaa gcgatgactg gagcgaagag ccaaagcagt gacattttgt
    10261 ctgatgtcgc acgctggcct gataagacgc gccagcgtcg catcaggcat cgtgtgcaaa
    10321 tgtcggatgc gatgtgaacg ccttatccga cctacaagac cttacatatc aacgtgttaa
    10381 aattatagat actgataaga cgcgccagcg tcgcatcagg catcgtgtgc aaatgtcgga
    10441 tgcggcgtga acgccttatc cgacgtacac gcgcttaagc ttcgcaatta ccggttcatt
    10501 ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccacttac cgggttgccc
    10561 ttctttaccc cacggctccc cttcccagct ttcgaccagc cagaaccaca gagttatatg
    10621 cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc
    10681 gacttcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca tttcaacttt
    10741 accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac gcgttataaa
    10801 gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt tcattgtctt
    10861 aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag cttttattgc
    10921 aggcggccat ggcactgctt gtatttttta ccagaaccac acgggcaagg atcgttacgt
    10981 cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc
    11041 tgatggctaa gctgctgcat ttgcgctaaa cgctcggctt ccatacgacg ctgttgttcc
    11101 agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact gataacttca
    11161 tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt gtattcctgc
    11221 ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata gtccatcgct
    11281 gccaggtgct ctttccacag agagtcgaga gtttgcagca tgacgccttt ctcgaagtga
    11341 cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat ggactgcgcc
    11401 agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag ccactcggca
    11461 attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg aatatcccac
    11521 atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tcgctttgaa cacatcttca
    11581 cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt acgctgggag
    11641 taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg cttacgaatg
    11701 tcgaagttac ggctttcaac tttacgctgg gcgttggcaa tcgctttggt cacccacggg
    11761 tgctcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga tactcggtcg
    11821 gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga agaaccagca
    11881 tccccctgac gaccagaacg accgcgcagc tggttatcga tacgacggga ttcgtgacgt
    11941 tcagtaccga tgatatgcag gccacctgct gccagtaccg catcgtgacg tacctgccag
    12001 tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc
    12061 tgccagctac caccgagcac aatatcggta ccacgacccg ccatgttggt cgcgatagtc
    12121 accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg
    12181 ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag ctccgatttt
    12241 tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc tttgatatct
    12301 tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg cagatcttta
    12361 cgaatcattg gacggttggt cggaacaacg acagtatcca gcttatagat ggagctgaat
    12421 tcgaaagctt cggtatcagc agtaccagtc atccccgcca gtttttcata cagacggaag
    12481 tagttctgga aggtgatcga agccagcgtc tggttttcgt tctggatctg cacaccttct
    12541 ttcgcttcca cagcctggtg cagaccatcg gaccagcgac ggccctgcat ggtacgaccg
    12601 gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac gtcacgggta
    12661 aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat gttggccgga
    12721 gagtacagag actctccttc atccatgatg ccttctttaa ccagcagttc ttcaatcagg
    12781 accagaccac gttcggtcag gttcacctgg cgagattttt catccaccga gaagtggcct
    12841 tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg aataatttta
    12901 ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat cagcggtgta
    12961 cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg cagtttacgc
    13021 tgtacacgtt cttcaggact gaacgccatg ttgtcacgca ggtagtcaaa gccgtattcg
    13081 ttgttcgtac cgtaagtgat gtcagcagcg taggcttcac gctttgccgg tgctggcatg
    13141 cccggcaggt tgataccgac agtcaggcca aggaattcaa acagcggacg gttgttttcg
    13201 gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt accggttagt
    13261 gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt acgcatttcg
    13321 gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa gtgacgcatg
    13381 ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat cagattttcc
    13441 agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt cagttcttcg
    13501 tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac tttgcgcatc
    13561 cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaataattt gattagcata
    13621 ataaaatctc aaacgccccg cgttgcggag ttaataaaat gaagtaaagg tttattgttg
    13681 ttagctgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc agacgggcgt
    13741 gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct tttcagacgg
    13801 cgtgccttcc tgggtcagaa gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg
    13861 aagaggcaaa gattcttcag caacgggcag cgtttgcggt gccattgcga aggaaagatg
    13921 gcggattacc gtgcgaatgg catgttgatg ccagtaatca acggaatagt tcgaattcgg
    13981 gcggcgcgtg ttggcttcca gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc
    14041 gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcattgc tgagtgcagg
    14101 caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt agcgtttacc
    14161 aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat cgtattcaaa
    14221 cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga atgaagataa
    14281 gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag ctcttataaa
    14341 attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg ccagtcgaat
    14401 tttatacgac agtataaatg tcgttatgcc agtacggctg aaggcgcttt gaaggccaac
    14461 ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt tgcaaggaca
    14521 gcctgcagca gtttgttatt cagtgcatga ccagatttat aagcggtaaa tgcaccaata
    14581 atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg acgcacaaat
    14641 tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac aacgatggca
    14701 caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat atcacgcatg
    14761 aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga gaagttcatc
    14821 gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt gaaatccaga
    14881 gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac acgaacagtc
    14941 tctttaatgc gaacaaattt cttggcgcag ttcaactcgt cgataccggc gtcaagcagc
    15001 aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc gttaacttcg
    15061 ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc tacggttgaa
    15121 atccgcacat catgctcgtt gaccagacac gtacagagca tggtatcacg cacagatttg
    15181 gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat gaccccggtg
    15241 ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa accgacaccc
    15301 gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt
    15361 acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa agagcctcga
    15421 aacccaaatt ccagtcaatt cttaatcagc ttgcttacgc aggaatgctg ggatatccag
    15481 ataatccggc tctttcgcag tttgcggcgc attgtcattc acgactttag caaccggctt
    15541 ctgctcctgg gtcagcggag ccataccgtg ctgctggtag cgatccatca ctggctgctg
    15601 aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga tacctgtcgc
    15661 aacaacagtt acgcgcagct cgtcattcat atccgggtca agagaagtac cgataaccac
    15721 ggtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga actcatccag
    15781 acgcaggtcg aagcccgctg tgatgttaac cagcacgccg cgtgcgccag acaggtcgat
    15841 atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac gatcttcacc
    15901 gctcgccaca ccagaaccca tcattgcgta gcccatttca gacattacgg tgcgtacgtc
    15961 tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac cttgcacagc
    16021 gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac ctcggcccag
    16081 aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct tggacagttc
    16141 agtgatcccc tgctccgcga atgccatacg cttcttgcct tcaaagttga aaggcttagt
    16201 gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga ctggtgctgc
    16261 acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg caccttccag
    16321 cgccgcacgc agtgcatcgc gatcctcatc agccgcattg cggccaactt ctggattagc
    16381 gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc caaccgctgt
    16441 tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt caatgcgctc
    16501 acgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga ctttaatcac
    16561 cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg tgcctgtcgc
    16621 ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca gccaactatt
    16681 gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt cagcttcacc
    16741 gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat aatacggctc
    16801 ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg
    16861 aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac cggttaatac
    16921 aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct gcaactgcaa
    16981 tatctcttcg ttgaccagat tgagcagctc ggtatagcgc ggctcgatca cctctgccag
    17041 ggtctgacgt tgtagactcc gcggcggacg accgcctacg ctcggcactt ccacgctctc
    17101 atcttttcca acgatggagc ccagcgcaca accgtggcga actttaatcg cttcggcgtc
    17161 acttggcggt gtgccaaagg cgtaagcgat atcactggtc acgacattgc cagcataagg
    17221 aattaccttc gtgtggcgca atgccccacc ggtataaacg gcgatatcca ttgtaccacc
    17281 accgatatca acgacgcaga cacccagttc acgttcatct tccgtcaata ccgaataact
    17341 tgatgccaga ccggcaaata tcagttggtc aactttcagc ccacaacgtt caaccgcttt
    17401 gacgatgttt ttcgccatat cattgtgaca tgtgatcagg tgcacttttg cctgcatccg
    17461 cacgcccgaa agccctaccg gattcttgat cccttcctga tagtcaatcg catactcttg
    17521 cgggatcaca tgcaacacac gatgctcatc gcgcacacgc accgatttcg cggtatggac
    17581 gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca taccaatttc
    17641 attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga tctgacaatc
    17701 tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc
    17761 gttcacccca cctttatcca taccacgcga cgggcaacta cccacgccaa tgatattgac
    17821 cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac caatctccag
    17881 tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt
    17941 tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc tccagagtca
    18001 taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc
    18061 ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat cgccccgacc aagattgagc
    18121 ttaatatcgt tattcagcgt caactgccag gaacgtcgcg cggtcatcgc cgcttccttc
    18181 agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg caacacttca
    18241 ttggcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt gcgatctggc
    18301 ggcacgctga aggtgtttcc ttccgcgtct accatatgtt gatcattcca ccgcgcaatc
    18361 ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct gacgctcacc
    18421 tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac atcctgggtc
    18481 ataaaggtac ctggctcacc caatgccagg atcgactgcc ggatatcgtc attacgcgtg
    18541 taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc atcttccatc
    18601 cagcccaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag caggaaaagg
    18661 atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc gctatttcgc
    18721 gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta ctaccaactg
    18781 cgagaagctc atacctgcct gacgtgccgc catcggcacc agactgtggc tggtcatacc
    18841 cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca taacgtcaat
    18901 acgtccccat cctttgcaac ctaacgtcgt ccatgctttt agcactaatg cctgcaaatt
    18961 ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat cagagagata
    19021 cttcgcctca taatcataga aggttccggc cggttgaata cgtattgacg gtaaaatttc
    19081 ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa tcaatacttc
    19141 ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg ctactacttt
    19201 tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca aacccagaga
    19261 agaaatttct gctaactgct tatcgttaag gcctttttca aactctgcgc gggttaacgc
    19321 tacccacggc gcgaccggta aaccggcacc ctgccacaga agtttgctgc gtagtttatc
    19381 cattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca tcagctcgag
    19441 catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa acactttctg
    19501 aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag gatacgcgtc
    19561 aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag aaacttcccg
    19621 ctcagcggag gtcccgccca acaggaccgc gattttatca gtcatgttgt tcttcctccg
    19681 gagtttgcgg cttcagtttg atttcagcta aagaacgagc aatttttccg atattaccag
    19741 ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc tcggctacct
    19801 gcgccgggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt gtgcgacaca
    19861 gcgaacggct gtccgctccc ggaattggcg cttcaccagc cggatacact tccagcatca
    19921 acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg cgcgtacggg
    19981 taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct gcgcgcgccg
    20041 ctttaatagt ggcgtccact tccgtcgggt ggtggccgta gtcatcgacc agcattgccg
    20101 taccgctttt tccattcact ggctctagcg ggaattcacc gaggaaatcg aaacggcgcc
    20161 ccgtcccctg gaagctttcc agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg
    20221 tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acgacctggc gcattcagag
    20281 tgacgcgcat cggctctttg tcctggcgca gcagcgtaaa gtgcccctgc gggccaatct
    20341 gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtc gtggtctgac
    20401 gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc accgcacgac
    20461 cgtaaaacgg caggttgtgc agaaaattaa taaaagtctg ttttaaattc tcaaagtcgc
    20521 cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc atcggttgca
    20581 gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga ccatgcccca
    20641 aacgcgcatg aacccccgcc gcttttacca gcccaccgtt aacgaaggtt gggtcgagtc
    20701 ccgcttctgc gtagatgcta gaaaccatcg cggtggttgt cgttttgccg tgtgttccgg
    20761 caatggcgat gccatgacga aaacgcatta actcagccag catttcagca cgacggatca
    20821 ccggaatacg cgcttcatga gctgcaacaa tttccgggtt atcggcagaa atcgcgctgg
    20881 aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg
    20941 ctcccagatt cattaactgc tgcgtgaccg gatttggcgc taaatcggaa ccactgatct
    21001 gataaccttc attggccaga acttcggcaa taccgcccat accggcacca ccgatgccga
    21061 caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt tttgccaatt
    21121 gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca aaaggcatcg
    21181 ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg tggcatccgg
    21241 aatggatgca gcgcgggcgc gttctgccat ggttaataag atttctcgcg accacccggc
    21301 cagggtgttg gcgacagcat ccacgctaag ctgtgactgc tcgataattt tggctgcgcc
    21361 cgctttttcc agcggtagcg cattccagta ttgctgacgg tctttatgtt gaaacggcac
    21421 aaacaacgcc ggtagtcctg ccgcggcgat ttcactcacc gttaacgcac cggagcggca
    21481 aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt ccgtcacttt
    21541 atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg aacctttgcc
    21601 gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg
    21661 attaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg gaccttcacg
    21721 tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca ccgggttacc
    21781 caccacttcc gcattaggaa aagcacctgg aaacgcctgc atcactttgg tagcaatctt
    21841 cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta caaccggaat
    21901 gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca taccgagcac
    21961 cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg
    22021 cagcggcgca gctatcagtg cttttatacc ttttccacgc agaccagaga tacgaatgaa
    22081 atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg cagtccccag
    22141 ccagcgaact tgccaaccct gagccattag atggtgcgca accgccagcc ccgggaatac
    22201 atgtccaccg gttccgcctg ccatcaccat taatcgcttc ccttgaccac tcatcgtgaa
    22261 cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg caacagcatc
    22321 atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgatcag cggcaatgtc
    22381 agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc ctggaaacta
    22441 aaccagatac caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat ttctaatgct
    22501 ttgcggccaa tcgacatcgc gcgaaaagcg acgaagaata ccattaaaag tgccagcacc
    22561 acaccgacat accccagttc ttcgccgata atggcgaaaa taaagtcagt atgcgcttcc
    22621 ggcagatact ccagtttttg taccgagtta cctaaacctt gtccccacag ttcaccgcga
    22681 ccaaacgcca tcaacgattg cgttaactga tagccgctgc caaagggatc ttcccacggg
    22741 ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag caacacaacc
    22801 gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc tgccaggaac
    22861 aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg ctgtgccagc
    22921 agtaacactg ccaacaccag aattacgccc atcggtttca ggaagccgcg caggttatta
    22981 cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag cgacagtttt
    23041 gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga tgccccttta
    23101 accgagctac ccactaccag aacgatcatc agcaggatga tagatccgag cagcatcgtg
    23161 gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc cagaatgaat
    23221 gccaggatca gatagacgcc atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt
    23281 tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc gaggccgaag
    23341 gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt ttcccgcgag
    23401 cccatcaccc agccctttag cgccgtggag atccagacca gaatactgaa tcctggcagg
    23461 cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc gccagacggg
    23521 caaactcatt gcctcgttgt tcaaagttct tgaattgatc aaggctggca caggccgggg
    23581 agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcatc gcctgttcca
    23641 tggtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg ccgtcccgac
    23701 cgaaacaata cagacgtaca ttatcgccat tcaggtaacg cgccagtggg ctaaagtccg
    23761 ccgatttacc atcgcccccc agcaacaaat gcagcgtgcc gtctacgtgc aggccattca
    23821 gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaacccag cgcacaccgt
    23881 tatgctccag cacaacttca aagcgatgcg gcagaccagt gaatgtggtt aacgctttca
    23941 ggctgctggc acgcggtaac cctgcggcat ctgccagcgc cagcgccgcc agcgcattgg
    24001 tgtagttatg ctgcccggaa agtttcattt ctttcacgtt cagcactttc tcgcctttca
    24061 cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcgcccatg ttgacgccaa
    24121 agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca tcagcattaa
    24181 ccacgcaaac ttttgcgttt tcgtaaatgc gcagttttgc tgcacgatac tgttgtaaac
    24241 caaacggata gcgatccata tgatcttcag tcacgttcag aatggtcgct gctaccgcct
    24301 gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac agttcgcact
    24361 catcatccaa cagcatcaac gcaggcaggc caatattgcc acccacacca acgttaaccc
    24421 ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg ttagaaccgg
    24481 taatcgccac aatcggtgct tgtgcttcac gacagaacag ctcgatatcg ccaacgattt
    24541 cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata ccgggactgg
    24601 cgacaatcag atctgccgcc atcagccatt catcattcag accgcccgtg tggcgttcta
    24661 cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata acgcgcggcg
    24721 tcacaccgcg agcgaggaaa aagtccacgc aagaaagccc ggtgaggccc aggccgataa
    24781 tgacgacatt tttaccctgg taatcagcca tgattaacgt accttcagcg ttgccagacc
    24841 aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg gttccggcca
    24901 gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag
    24961 tttaaaggag ccgacctgca ggatgacgga aagcgtttct accacgaaca cgccccccat
    25021 aatcaccagc aggaattcct gacgcagcag tacggcgata atacctaacg caccacctaa
    25081 cgccagcgaa cctacatcgc ccataaagac ctgcgccgga taggtgttaa accacaggaa
    25141 gcccagccct gccccgacta tcgcggtaca gacaatgacc agttccccgg cgtgtcgtag
    25201 atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg ccaccagcgc
    25261 aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcgagac catcggtcag
    25321 gtttaccgcg ttgccagtac ccacaatgac aaagtaagcc agcagaatgt agaacagccc
    25381 cagttgcggc atcacatctt taaagaacgg aaccaccagc tgcgttgcgg gcgtgtcttt
    25441 gccggcaagg tacagcgcga aggcaacacc cagtgcaatg accgacatcc agaaatactt
    25501 ccaacgggca atcaggccct tggtgtcttt acgcaccact ttgcgataat catcaacaaa
    25561 gccaataaca ccataaccta ccagcaccac cagtacgcac cagacgtacg gattggacgg
    25621 gtaagcccac agcagcacgg agatcacaat cgccgtcagg atcattatcc cgcccatggt
    25681 cggtgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacca cctgaccaaa
    25741 ggaaagtttt tgcaaatgag caatcatacg cgggcccatc cacaatgaga tgaacagcgc
    25801 ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa agccggaata
    25861 atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg taaagcgcgt
    25921 actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat tacctgttgc
    25981 tcagcaatca gtgatttaag acgcgcgata agcgcagttt tatcggaaaa atgttcgcca
    26041 acgccgctgg cggtgctgat agcatggctt tgtttaccca cacttaacac gcagtcaata
    26101 ccagcagctt ttgccgcttc gcccacctgt acatggcagg cttcgctttc agcgcccagt
    26161 tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag aacctgaaca
    26221 gcggcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa ctggttttct
    26281 gccagtttta tggggaacag acggcctgga acagctttca gatttgccag ccccgctttg
    26341 atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag cgcattcgca
    26401 atattgtgac gccccggcaa cggcagcaga acatcgacgc tacctgtagg ggtttgcagg
    26461 gtaaattccg taccgtgcga ggttacatgg atattggtgg cggtgaaatc gctgttggcg
    26521 gcattaggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca gttcagccag
    26581 tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct aaagatttca
    26641 cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccaaatgcgc cgctgccagg
    26701 ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat ttcaccctga
    26761 tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg caacagcgtc
    26821 atcggtacac cgatgtcgtt attgagattg cctgccgtat aaagtgtgtt gccgcactgg
    26881 cttaaaattg ccgccgtcat ctctttaacg gaggttttgc cggaggaacc tgtcagagca
    26941 accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa cgccagacgc
    27001 gtatccttga cgattaactg cggcagatca atatccagcg gacggctaac cagtagtgcg
    27061 cctgcgccgc cagctttcgc ctggtcggca aaatcatgag catcgaaacg ttcgcctttc
    27121 agggcaacaa acaggcagcc cggcgtcagt tttcgcgtgt cagtggttac agcatcaagg
    27181 gtaatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct aagggttacg
    27241 ctaatcatgc aatcaccccc agcagacgcg ccaccgtgac gcgatcggag tagtccagac
    27301 gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt accacatcat
    27361 tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc atcactttgg
    27421 catatccggc atctaacatt cccgccagaa tatcgttgat gatggcacga ggctcttcgg
    27481 tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcagca attgcgccca
    27541 tcagtggacg tttgccttta tcacgatcgc caccgcagcc aaagacgcac cacagcttgc
    27601 ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt
    27661 aatccaccac caccgtcggt ttgcctggtg cagtgaacac ttccatacgt ccgcaaaccg
    27721 gttgcagacg cgcggcggtt ttcagcaaat cagccagcgg atagccgagt gccagcagtg
    27781 tcgccagcgc cagcagcagg ttgctgacgt tgaatgcgcc catcaggcga ctttcaattt
    27841 ccccatcgcc ccaactcgag ctaaagcgaa tggtcgcacc gctgtcgtga tagttcacgt
    27901 cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc attgataccg
    27961 caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg gcgttaataa
    28021 tcgcctgacc gcaatgatgc tcagaataaa gcagccattt cgcagcttcg tagtgttcca
    28081 tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac gccgcaaatt
    28141 tcaatgccgc cacacggtgt tgtaccagcc cgtgggatga aacttccatt gcgcaaaaag
    28201 tcgcgccctg atccaccagc cccgccagct catgctgaac atcgactgcc gaaccggtgg
    28261 tattttctgt cgggatcact ttccccagca ggccgttacc aacggtgccc attaccgcgc
    28321 tggtttcgcc aagcagttgg ctccactgtg ccagcagctg ggtcgtcgtg gttttgccgt
    28381 tggtgcccgt tacgcctaca agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg
    28441 ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg ccgtgcattt
    28501 cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca gccacacctt
    28561 gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact acagctacaa
    28621 agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt gctcgcgaag
    28681 gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc acctgtaccc
    28741 tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc cggctcgatg
    28801 ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga aacggcgccg
    28861 ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa gcgcggctga
    28921 ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg accgtccggc
    28981 ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat cgccgccttc
    29041 acgccgccgc cgcccggtag cgccacgctt tccatcatat gcaccacggt gcgaacaatg
    29101 gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat cgacagtggg
    29161 cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa cggtgttacc
    29221 attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg ttgtttttga
    29281 ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc cagtccaaaa
    29341 cgtgagtaag tatctactaa cgctgaggac ggcatcgcta acgccagttt ggaaacaccg
    29401 acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg cgccacgtct
    29461 ttgatttcgt gaccgttaat tcgataagga acggtattga gtaccgagtt ttcccgcacc
    29521 acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc cggttcaaac
    29581 acgtcggtga tggtacggtt acgcatcgct tctttcggcg tgccgctcag attatttggg
    29641 ttgtatgacg ggctgttagc catcgccagc acttcaccgg tgttgacatc caccagcacg
    29701 gcgctaccag attcagcctt gttaaaggcc actgcgttgt tcagttcgcg ataaaccagc
    29761 gcctgcaggc gttcatcaat actcagcgtc aggttgtgcg ctgcctggct gtcagtagaa
    29821 gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc acccggctgc
    29881 ccggtaagcc atttatcgaa actcttctcg acgccctcaa tcccttggct atcgacgtta
    29941 gtaaagccga tgaggtgagc agtcacttcg ccggatggat aataacggcg agattcttca
    30001 cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc agggtttacc
    30061 tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc tgagagctga
    30121 tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac gctgataccg
    30181 ccagcgtcat gcacttcttt tgggtcagcc caaatcgctt ttactggcac actcaccgct
    30241 aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg ctgaacgcga
    30301 agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg taaccacgct
    30361 acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa cgcaaaacgc
    30421 caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt cgccgctgct
    30481 ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga cggatcaaca
    30541 tgctgcattt gcagcttttc cgtggctatt ctttccaccc ggctatggtc gccgagcgca
    30601 ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc cagcaccagt
    30661 tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac ggtcaccgcc
    30721 gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa aagatcgtca
    30781 ccgataacgc caggcaaggc atggcgctcg tggcttccca tcgatccttt aactttgctt
    30841 agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc agaactgaac
    30901 tacgggcacg agggttctca gccacctctt cttcgcccgg catcaactta cctagtgctc
    30961 gcaactgacg gccccccagt tttttgagct gctcttctgt catcggtaac cctgccggaa
    31021 cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatgcggtct tccagcgagt
    31081 ggaagctgat gatcgaaagc cgcccacccg gggccagcac attgagcgag ctttttagcg
    31141 cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg aaggtacggg
    31201 tcgcgggatg tttaaactta tctttcaccg gcgttgcggc agccacgact tccgccagtt
    31261 ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg gcaatgcgtt
    31321 tggcaaaacg ctcttcacca taggttttca atacccaggc gatatcggct tcttctgcgg
    31381 tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg tccagcggac
    31441 catcgcgcat aaaggaaaag ccgcgctcag catcatcaag ttgcggtgaa gagacgccaa
    31501 gatcgaggag aatgccgtcg atcttgccga taagatcgcg ctcggcaacg tattcgccca
    31561 gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc ttcgcaacgg
    31621 cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca agctgcgaga
    31681 ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg ccatcaggac
    31741 ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta tagttttcca
    31801 tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc caactgctct
    31861 gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag ctcaaacttg
    31921 ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg cagtactggc
    31981 gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc taacagtagg
    32041 cgctgcacac ggcgctcaac cgggttcatg ctcgacagac gcgataattt ttgctcgata
    32101 atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc aatggtgcaa
    32161 accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg cactgataag
    32221 cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc ctcacccctt
    32281 attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga ggaaaagctt
    32341 gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc taatatcagc
    32401 gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc tgtaagaaaa
    32461 aatccctcat cgtcatcacc gttttaaaat gactcactat tttccacagc aaatataaag
    32521 tgtcagtttg cgacgcgagc ggcattttag gacatatctt ctctggttaa cagtccctgt
    32581 tgcgtctgtg tggcgcttga tgccagcagg tttgcgcaaa aacaaagaaa agtgtttgtt
    32641 aaatcggcaa ttcatctctg catgtaacaa aataatgcaa aaacgcgctg tcaatatcat
    32701 ttaattattg tgaatttcag caggtgagtt aattaacggg cgggtttaaa agcatttatc
    32761 aataatagcc aggaatagtc ttatttactt taaggatatt gatgtccatt cccgtactca
    32821 acgcgccaga gagaaattca cctggcgcgt acttttgttc gcggcttagc tacggctgag
    32881 cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa ccaggttttg gcttacgcgg
    32941 ttcatcgagg ctcgccagga caatctccag cacacgttct gcgacatcgc ggtgacgttg
    33001 agccactgcc agcaccgggc actgtaagaa gtcgagcagt tcgttatcgc caaaggtggc
    33061 aattgccagg tcagaaggca gtttgccgtc gcgacgtagc gtgacatcca tcaccccttg
    33121 caacagcgca aacgacgttg tgaacagcgc ctgcggcatc ggatgcgttt ccagccattt
    33181 ttcgaataac tgggcagctg cctcccgctc atagctgttg gcataaagga aatgcacttc
    33241 gcgcggatca tctttccagg cagtacggaa gccttgttca cgcaggaagc tgacagaaag
    33301 ctccggcagc gcgccaagat aaagcaccgt ctcggcggga aacttacgta actcttccgc
    33361 cagcatttcg gcatcatcct gatcggcacc aaccacgctg gtgaagtgtt cacgatcgag
    33421 ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc cagcgttgat aaaaaggatg
    33481 ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc tgacgctgta aaaggtgctc
    33541 aatgcagcgc atttcgttgt cgggctgatc ttccgagcag gcaatcagca gttgataacc
    33601 ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg gtatagctgg tgttctccag
    33661 atcggggatc acaagaccaa tagaacgtgt gcgtccagca cgaagcccag ctgccacggc
    33721 gttcgggtgg taattgtgct cacgcaccac agccatgact ttttcaacgg ttttgtcgct
    33781 cacacggtat tgcttcgctt tgccgttaat aacatagctt gcagtggtcc gcgacactcc
    33841 cgccagccga gcgatttcat ccagtttcac aattgcccct tgcgtaaaat gtaaaaacca
    33901 tagccattgt actggtatgg attaaattca ttaacatcta agcgcagaaa attaactgcg
    33961 gcaactgctt ttattccagg ttcccactga tttcgcaaaa aaagcccaac gtgacatgtt
    34021 gggctgcaaa ttgcgcattg agatcattct caacgcatta ttttatcgcc gcgcgaaagt
    34081 ccgaccacac cagagcgagc aacttccaca attttcgcca catcgcgaat cgatgctaaa
    34141 aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg tataaagcga gggtgtgaca
    34201 tcgataattt gcccacggaa tatttccgta ttacgtttca cttcgtcacg cccgtaaccg
    34261 ctggcctgaa ttttcaccag catgatttcc cgctcaacgt gcgcgccctg ccccaactcg
    34321 ctcacgcgca agacatcgac cagcttgtgt aattgctttt cgatttgctc aagcactttt
    34381 tcatcgccca cggtctggat ggtcatacgc gataatgtcg gatcgtcggt tggcgcaacg
    34441 gtcaggcttt caatgttgta gccacgctgg gaaaaaaggc caatcacgcg ggataacgcg
    34501 cctgattcat tttcgagtaa gactgataat atccggcgca taatcaggtt ctctccgttt
    34561 tgcttaacca catttcatcc attccgcccc cgcgaatctg catcgggtag acgtgctcac
    34621 tgccatcgac ggtaacatca acaaacacca ggcgattatt gcgcacctgt tccagcgcct
    34681 cgctaagttt gctttccagt tcttgcggat gagagatctg gatcccgacg tgaccatagg
    34741 cttccgccag acggacgaag tctggtaagg actgcatata agattgtgaa tgacggccgg
    34801 aatagatcat gtcctgccac tgcttcacca tccccagata gcggttattg agattcacca
    34861 ccagtacggg caactcatat tgcaacgcgg tagacagttc ctggatgttc atctgaatac
    34921 tgccgtcacc agtgacgcaa accacggttt cttctggcaa cgccattttg acgcccagtg
    34981 ccgcaggtaa accaaaaccc atcgtgccga ggccgccaga gttgatccag cgacgcggtt
    35041 tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg cccgacatcg gacgtcacgt
    35101 aagcgtcccc atgagtcaac cgccaaagag tctcgatcac cgcctgcggt ttaatctttt
    35161 cactgtgagt gtcatatttc aggcactgac gagcgcgcca ctgttcaatc tgctgccacc
    35221 agtcgcggat ctcatccagc ggttgatggg cggattcttg cgacaggagt tcaagcattt
    35281 gttctaggac caggcgagca tcccccacaa tcgggatatc agcagtcacg gttttagaaa
    35341 ttgaggttgg atcaatatcg atatgcagaa cagtggcatt tgggcagtac tttgccagat
    35401 tgttcgtcgt tcggtcatca aaacgtaccc cgacggcgaa aatcacatcc gcgttatgca
    35461 tcgtcatatt ggcttcgtag gtaccgtgca ttcccaacat gcccagtgcc tggcgatgcg
    35521 ttgccggaaa cgcccccagc cccatcaaag aggaaacaac gggcagattc aacacctcca
    35581 ccgcttcttt caactgcaga tggcagcccg ccgtgattgc cccaccgcct acgtagacaa
    35641 ccggtttttt tgccgccacc agcgtttgca gagcgcgctt aatttgccct ttatgcccgg
    35701 aggtcgtggg attgtaagaa cgcatactga ccgactccgg ccagacatag ggtaatttgt
    35761 tcgccggatt tagaatatct ttcggtaaat caacgaccac gggtccagga cgaccacttg
    35821 ccgctagcca gaaagccttt ttcagcacct gcggaatgtc ttccgtttgc ttaaccagaa
    35881 aactgtgttt aaccaccggg cgagaaatcc ccaccatgtc gcactcctga aaggcatcat
    35941 aacctatcaa cgaggtcgct acctgcccgg aaaggacaac taatggaatg gaatccatat
    36001 aagcggtggc aatgccagta atcgcattgg tcgcccctgg acccgacgtt accagcacga
    36061 cgccgacttc cccggtcgcg cgcgccagac catcggccat atgcaccgcc gcctgctcat
    36121 ggcgaactaa aacatgatcg ataccaccca cggtatgcaa tgcatcgtaa atatcaagga
    36181 ccgcgcctcc aggataaccg aatacttgtt taacgccctg atcgataagc gatcggacga
    36241 ccatctcggc tccagacaac atctccatgg cctgcctcac tgtttgacgg aaaaaatgtg
    36301 taaaacacat taaccgtccg atcttgctta agcaattggc agacagagac ataaacacgg
    36361 ggtaataaga ataaacagga ggaaaggtga aaagaaacga taaaatcctc cattacagag
    36421 gatgaaattg ataataaatc ggctgaa
//
LOCUS       DADTFG010000028        36348 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.028, whole genome shotgun sequence.
ACCESSION   DADTFG010000028 DADTFG010000000
VERSION     DADTFG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.028"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            144..443
                     /locus_tag="J1138_001348"
     CDS             144..443
                     /locus_tag="J1138_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453170.1"
                     /translation="MLNISVAKYIVKEFTSKQLNDLNELSQKLIEELKELPEREVKKG
                     IRRSPEEVKSFILEIMEKNPGISATHALREFRDSGNSFEEKRFRTEFMALREAKP"
     gene            517..1767
                     /locus_tag="J1138_001349"
     CDS             517..1767
                     /locus_tag="J1138_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HBA8453171.1"
                     /translation="MSKLKYFFPDSQDFIDPSFDFFRETRNEHRVRQRDDQYPHEVFP
                     HPYDGMLVSKAVVDGLGGGESKYTRAQRLRYFRNGMKHFFRLPENMETMGDCGAFTYV
                     NQDVPPYRVEEVIEFYETSRFNHGVSLDHIVFGYEKPGEVFSGEVLAECRRRQDITLT
                     LAQEFLNKSQKSCFTPFGVAHGWSKTSYRQSVEALLAMGYRNITMGGMVPLKTAQILE
                     TLEEIKPLLKSDTRIHLLGIARPESFADFIKFGVTSIDSTTPLQQAFKDRKNNYHTPD
                     GPAYTAVRVPQFDANPSLSRKIKSGVIDQDIARHLEKDAMAALFEYDKGALSLEKTLD
                     AVLAYERLHSGEKEADKIRADYERTLGERPWNQCQCNICQAIGINVIIFRGAERNRRR
                     GFHNIQVLYNRLQHTLSLRSEELS"
     gene            1764..2897
                     /locus_tag="J1138_001350"
     CDS             1764..2897
                     /locus_tag="J1138_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HBA8453172.1"
                     /translation="MSEYRVPALRIRQGEERQLYSLAIEGKQISKIAAISRIRRGEES
                     LVGYQRPEVRNHIREIQRYIESKNPMIPNPVIIAFDKRVRFEPLTDNGDMGHLVIPFS
                     EDKDFEKPGFIVDGQQRTAALRDAEIDSFMMPVSAFIANDAEEQREQFMLVNSTKPLP
                     KTLLYELAPHTHGRLPSDLQMRKFPSLLAQRLNFGEGPLAGRIKTATNPDGVIADNSM
                     IKMIDASLREGALYRFRDPATGLGDEGKMVKLLNNFWSAVETVFTDDWDKKPRYSRLL
                     HGVGILALGSLMDEIDQVHQDYKGEPGWTEIPSYTRFVEELNRIKPLCAWSGGVWNFG
                     TDIDGQPIVRKWNELQNLSKDISLVTDFLVMNYIKSVNADINT"
     gene            3490..6270
                     /locus_tag="J1138_001351"
     CDS             3490..6270
                     /locus_tag="J1138_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA8453173.1"
                     /translation="MVIVRSDKNVVIDLADIFYPNYGQGLAMNPAHFLAARANDAPFF
                     YPLREKFVSAWIKQRAACRSMSSLISSRVEIEPHQIAIVRRILQDQNPKYLLADEVGL
                     GKTIEAGLVIREHILECKREARVLVVVPQVLQGQWTQELVDRFALQEVMSGGRHTQIR
                     LCLPEELCQPELIGWKPTMVVIDEAHQLTRQAWADDEDDLAQYAAMAALCHQARIVLL
                     LSGTPMHGNERHFLAMLHCINPQAWQLNAQGIEKFMQRVAERERLGGIYSSLTPETPN
                     GVLEEILEELRLLFADDEQLQARIVGLSPLVDFFAPDESEERTAGILALRQWIGERYR
                     LFHRLLRNRREDPALEYLFPGLAGLEKCVWPVASTEITLDETLDAWRAAAWRNPAQHQ
                     HLDSTTLDEWVEALFLNPLVMSRQAKKVLVKGTASEEEFNFLEQIIETSQQEQKAKDV
                     ALMKALTDWFEKCPDGKAVIFCGEGSEAAFLFTKLQIQAPWAVVRHAPDQRKQSELLD
                     DSWQVLICDRRGEDGLNLHGNNRLAVHYSLSRDFNRFEQRLGRFNRYSGNLRGVKPVK
                     SLVLLPERDGLRADWVKLLDEGTGLFHRSVASLQFVLSEQLDVVWRDYVGQGLAVFHE
                     AQQRFSGENGFIAQERKRVLAQENLLSMEQEVIAAREFSEQLAESEDDAEEQAKDMLA
                     WMCKALGFKREKYPEGGFRLRFERGEYQRQTLVDVGTFIDNCLLGLDFSEGYPPSTAM
                     MSLSRTEAGNHKHVYPLRYGQPFVETVWQLMQSDPRGASMALLRVLSSAVALKQPHTW
                     FHFQWLSEAQVEGENQLAAQRRGDECFSPVVHNFWLDDGGKEADPQIVVSLLDKPYDE
                     EGNRLFQDINLREEVWTRMPDWFDPHSWKETVLAAAEQARQNVHAHYGDRPVRHQLLA
                     MKAIILCTRDML"
     gene            6283..8733
                     /locus_tag="J1138_001352"
     CDS             6283..8733
                     /locus_tag="J1138_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBA8453174.1"
                     /translation="MENQWQQLRAVLNGELNERDGLSGQFFNRLLYCLDDVRAGECDR
                     LHAFYDALQCARALGLTPLRLPYRHLTEGTMLQHFGLRRDHDYPGELLLTREAEEIDP
                     HLLPVWKGEQRRFLETPPLDVLLPSVLNDPRYTHYTSAGQQQAVRTVLTCGRDKTLLV
                     NLPTGSGKTFVIHSQMLTSPRRHLTLVIVPTVALAIEQALRAQEVMQQAGQDHGGRYV
                     WHSGQSTEERNEMKSRLASGEQRALFCSPEAATGGLLLQLFSLAERGLLGAVIVDEAH
                     LIDQWGVEFRPEFQLLAPLVRSLKATSPDGVKVVLLSATFSQSTLNTLKALFAGKRQG
                     SVIEVNGSFLRPEPVWYVSEAASHEDHLAQVENAIARLPAPMILYTTEVEQAKFWYQY
                     LRERGYRRCGLFHGATPMQEREGLIHAWRNDGLDIMVATSAFGVGMDKNNVRSVLHVA
                     VPENLDRFYQESGRGGRDGKASVAHIIFHHKQLQVARNINRTKLIGASKGYLRWAKMH
                     QLREQHQPGQFIVPLRAKHADIRMDSQSNVDWNLRTLLLMQRAGFIDIAYPPPDLSAI
                     APDERDEAKIRAWFDHYFNHIQVSVRRDGHMDEARWHEGIQAHRSHELGMRKQGYSAL
                     EEWLNDPSISLCQKLAQFYTLDGFVPEIACGGCPACRSKGYPPFTPTLGRIAHVTGEA
                     VRNVMGNEQRVYYPATLTNRLLLRQWSDWIARLLANRQIQAIRASQSVLAQLSAVLPA
                     GLPFWCSLAVDEENTCWDELVLVLPDEAMPELDIFATINRIIVAPERLQEPGYRGRRW
                     WEVDTSAVALEQFQRNII"
     gene            8733..9632
                     /locus_tag="J1138_001353"
     CDS             8733..9632
                     /locus_tag="J1138_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453175.1"
                     /translation="MAITNNAAAGSQIRLLCMIDRVLNRRTGQAIPMADLIELLRPEM
                     LPVSDGARKRLPAEVSFWTKEGLWKNESAGLSQQSPLSSERNLPARVLRTLINSEKTT
                     PLLTGTRGQPLLMCVTSMLAQEKYTLRGSTPLTKEAVPLAVGPMINNKLAGEGRRNLN
                     VSNEAEPFLNYAYFLGFTEPYLNGWVMDPTRAIEGILDNLGLTAGMPIQEFLSQLAEF
                     LPMLDGGAYRQQVEPMISADNWQPLEKHMISASLSQALLRLELTMQLVFSSRSDDLDA
                     MVLQGPDGSLRRISTVSPGEARK"
     gene            9635..12778
                     /locus_tag="J1138_001354"
     CDS             9635..12778
                     /locus_tag="J1138_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453176.1"
                     /translation="MIEYWPSLKNINECIRTEAEELAEYTLLAVHQPVNLLRRDKDGN
                     NLGYAKEEDLLNHFLETPRPIPVVGKAGVGKSHIIRWLDAKLRLRQEYKDKKWHIVRI
                     PKSASLREVLTSMLAGLEGEIFDEAREDINKVSDKRTPREIAEWLLMLMGQELRNLHE
                     RNKQDMEALKKQMAGSTPEQLKAMKPQATRLQKIHIHAEDNALPTLINDAYFKQFLLK
                     EEHCLYRLASRLTNGATSSDLDEGEQQLQAKDLDFSFNINDLSLPARQYIQKSRLNTH
                     EDGRKDASEILNLVLGKATQALFNQLFNFRGRSFSDLFTQIRQALHEKHMTLMVLVED
                     MSLITAIEDVLIDSLEREGIRDGKEVLCPVCSAIAVTDGYQGYARRRQGMLDRAKGEW
                     VIEEVGSGREETRLRIVDFCGRYINAARFGSEALLQRWEQAADKSHWPPDWEASANGD
                     EMAEVFGRSEQGFSLYPFNASAIHALAEAFCRDDRNELKFNPRQIINQILLRVLQHCR
                     RDADEGRFPPPRLGDIAAPAGLRSWLFRQGFADTERAESVVALWGYPADSDATLASTL
                     PPDVARSFDLEDLAQVLENTKSAPLVEQSVMPTRVTKVEQQVKKTVQPPKPVKPVLKE
                     DKDTLAIRALDAAVGDWMLKGAPLEIDPARYIKSSLAFFFDKRAVAEWAGSSYRPTLW
                     LGKSNFVAVELPNAQGNRGVHVVKFISQSEYDKRSVQLTDAAMALARFGYYRENSLTK
                     TEDWSYPEGKTDYLIIQSFCDRWVNYALTELVKHKRNDLPLLLSEQIALADALGVIKT
                     ADGPKEVLGRLLQNSKTLSSQFRTGITKAITELRGEALAKWDDAQDAWLSLVALNDHA
                     LEGDLLLSAIQKVLKKRSNNTHAAVVKKSLSEIRPALDTAALFADCENADDFSELVTG
                     LAQLVKSLGDSGDYPADMTPDSSTLANSLTGLTEGGVWLTILKLRGITQSEDPLRQWQ
                     LLCELDGTLINRLIFTISGWQQVNKRVLANITAYNHSHGSHQISEFRTQIESTLQELH
                     QVLDAMQSVAGEQYDNA"
     gene            12765..13499
                     /locus_tag="J1138_001355"
     CDS             12765..13499
                     /locus_tag="J1138_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453177.1"
                     /translation="MTTLKTQLTQLREQYQIKQQVQSLEEVYDRYRTIRDGLLDVAEP
                     LQKAQKQLEVIGTLPEPHSAIDFSQEAALFGGAKTQLDALTARFKESGDKTEQCGFWE
                     TVRVLKSVHESIDDKVDATWKAFVADLEARATLDPVFLEQQRTLGNVLVYDDYRKARD
                     NFNRQKLSGPDSLSVVKNLQKYSDEMIALKRSMDLDAPDDVLRFFEALDRHNHASLTL
                     LTPGVITWLEEKDMLPAFNVTRKRMI"
     gene            13499..18013
                     /locus_tag="J1138_001356"
     CDS             13499..18013
                     /locus_tag="J1138_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA8453178.1"
                     /translation="MKGIDLSLLQRVLDALEEQENALLVWGDTGGFFNLDELLSVIGR
                     VLPDEDAEVVLDQMLEAAMLIQVPHVSGTPVWRTRMGETVHLMRNLRQWMHKQKLDQS
                     KTLVSDYRFIRRPRSYPDRVYEPATLISGWKKQLKLSDRVCDIMRQALASDKPFLLAG
                     FQVRATERIMQCWQDHQVKSNTASGTIVCAGTGSGKTLSFYLPALTRLAEDICNNPER
                     KVRILAIYPRKELLKDQFAETFSQCRKLDDYMLTAAGRKIRIGAFFGDTPVKAEWSKE
                     DVKGKVGLPFGLMKCQQPHPHHPKQRCGGALIWRREDVFGEREVLTCTQCQHQVDQSE
                     IMITRDAQQHRGDAPDILFTTTEMLNLQMNSTWSNHLFGVGEGYGPTLVLLDEVHTYS
                     GTTGAQTAFLLRRWMQRTDCLPHFVGLSATLTDAQHFFARLVGAAEEQVALIQPYMED
                     MIEEGAEYLLALRGDPVSETALLSTTIQASMLMARMLDSKANKSQGTWGKKTFIFTDT
                     LDGNNRLYHDLSDAEGWETGPGHSRIDHSPLAVLRSPFDNTAPERSKTELGQNWRAAT
                     DIGHDLAENKVISRTSSQDAGVDASADVVVATSSLEVGYNDPLVGAVLQHKAPNDVAS
                     YLQRKGRAGRPRGMRPWMLVVLSEFGRDRVEFQRYEGLMSPEIKRQGLPLDNQHVQKM
                     QAAMATLDWISKVGQFKDLWSMLKKAEHNQLKYNRMYGPLIKLIEEVLSGGRRLNELM
                     RYLQDALQLSDGAVQNILWSPPRSIMFEFLPTILRNLRTRWSVNGVEWAGLRPNQPNS
                     EGEQHRSNSPVPEYIPQNLFSELNLPELDIRLKRGFDDEDHWETLSFWQGIREFAPGR
                     LSKRYAVKSNKSTDWLVPQSYEPMAGEGRQFVDFQISDAFGDSWQNEYEVDYMGKTIK
                     VVKPSKVMTTRADIRRINDKSNAQLQWVFNVINPAIATPDEVPKGPWKHTLSDVTFYN
                     HQHMTPLELVRFSTGSQASLRFRNKERAHVDFTWVNGEEQVGIGSRQWVDAMRLRFNL
                     TCDDVLGLLHQEEIQRGMRPVYFQHLVRQSPEFEFDSFNADWAIECFMAQLAETLANG
                     AHASVESALREMASEKGGERLADIPASLFQPDTDNETGTDQALQIGLNKLLQRPEIQQ
                     LLLNCAQALWKPLDEIDGFVEWARQVLADTLAAGVQQTLSTLLPDVDERAVVTDSSWM
                     SDPRKGAEWLEIWLCEMESGGSGILIRLQQKWAEDPVSFLNVLVRNLSASDYEQIDYD
                     LRTVLQMMQTDDTLRMAISAVREASNMDARREANKNLHLQLSQRGMRLSHSFTTVLYS
                     RILRAGSGEHTDTQLYQLLSDWSSLEASAGIEFSMNTMAHALAVNSLGVETDASVVFD
                     EQCRNQNLLWPRGYTIRQAELGFYNMFCSRKVTTERLLAGALFSEQIEKIALDDDWLG
                     HLHAALRKGGRAELILTRQQRNQLHQVITTVQIEPVDHLGLLLYPRLGEVRREQDVLI
                     LRIELAEAMQ"
     gene            18010..18555
                     /locus_tag="J1138_001357"
     CDS             18010..18555
                     /locus_tag="J1138_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD-like domain protein"
                     /protein_id="HBA8453179.1"
                     /translation="MIGRAKQRQIFLHGSAGHRQLREVLSAQVVALIMQPEPIWLVSP
                     WVSNFSLLDNRSGNWDSIDPAWGGREVDFIELLACAVNNGAPLSLVTRDEPMNRIFVK
                     ALEKRLSNFATFRLEWRNQLHTKGFLTPNFFLKGSMNFTRSGVNRNEEFVELNCDSHM
                     IGEAMAEFESQYLFIGSKEAF"
     gene            18555..20726
                     /locus_tag="J1138_001358"
     CDS             18555..20726
                     /locus_tag="J1138_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453180.1"
                     /translation="MCADWLKNYYAEDNYLDYDKAMQGGYGIPQMNTLIQQAAALRMP
                     CIVPSTRTGRTVFYALAENAKSLDELRRILTAALGSADTTPDIKSLYHSDDGGEQLLL
                     EKSPDGILAFDFLPVPDGSPQQVKEWQAARMKRVYAMLQTVMDLYHQRPVLHSLVSRQ
                     TGRILRDFYTACQARDGKIAEQYLEELRGNQTLSSLNLLFLELQGMAASAKWDAILNH
                     PRLEVLLRGRVPERIQRLLLRSSGHLMLNAIRDAHFPLDRREDARRLVLGLLPLYKHK
                     PRFAHQASFLPDWQLWAMGAALLGIDEWQTATPLLAPDWIQQVEGWATGTTSLPAPVA
                     AEEQALIQAPVIMLISLENATDLLLEALLADAERESEIYAQLAAMPEATRQALQKIPK
                     LWEAWQALKKRCEPQDYGWCRWLADLQQTTESERFESLRQQATVHYMDWAPSTFSETQ
                     WQALLEHQSNTQLSKVLRDVLPTLLNWLEEYDVKVSASLWPDWLMLLAVEDIRSEEDV
                     RLGGMILDKFLSGPFTCEEYSAAIESTEILCAENVSVRTLGYSLDIAELLYDRVTANE
                     ASRLGFWIKLQELLKHRWERLDASMQFSARMVERLYLGEHAGNVFPSEDNTPGVASSL
                     HRDLSGKTLAIYSLMKGAARRGKEALLQLYPGLEVELNHDHVATPALVNLAKTADYFI
                     FASSSSKHQAFYAVTDYRKEIIYPSGKGASSMIASFVRAME"
     gene            complement(20989..21351)
                     /gene="queD"
                     /locus_tag="J1138_001359"
     CDS             complement(20989..21351)
                     /gene="queD"
                     /locus_tag="J1138_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBA8453181.1"
                     /translation="MKTTLYKDFTFEAAHRLPNVPNGHKCGRLHGHSFVVRLEITGEV
                     DKHTGWIIDFAELKAIFKPTLDRLDHYYLNDITGLENPTSEVLAEWIWNQIKPQLSIL
                     SAVIVKETCSAGCIYRGE"
     gene            complement(21602..21991)
                     /locus_tag="J1138_001360"
     CDS             complement(21602..21991)
                     /locus_tag="J1138_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453182.1"
                     /translation="MFAKSFIALDGNDRLTGARTAQTAPYDRYTCHLCHSALKYHPEY
                     DTERPWFEHIDAGSAENGQHNCPYVKPDASEIHLARRLQRFLPDATPRVYKADWNCIQ
                     CGKNYYGECYCSACRTGDFSQLTKMGS"
     gene            22241..22498
                     /locus_tag="J1138_001361"
     CDS             22241..22498
                     /locus_tag="J1138_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453183.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(23056..23823)
                     /gene="fecE"
                     /locus_tag="J1138_001362"
     CDS             complement(23056..23823)
                     /gene="fecE"
                     /locus_tag="J1138_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBA8453184.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGKLRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(23824..24780)
                     /gene="fecD"
                     /locus_tag="J1138_001363"
     CDS             complement(23824..24780)
                     /gene="fecD"
                     /locus_tag="J1138_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBA8453185.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGMLTAIIGAPWFVWLLVRMR"
     gene            complement(24777..25775)
                     /gene="fecC"
                     /locus_tag="J1138_001364"
     CDS             complement(24777..25775)
                     /gene="fecC"
                     /locus_tag="J1138_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBA8453186.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(25772..26674)
                     /gene="fecB"
                     /locus_tag="J1138_001365"
     CDS             complement(25772..26674)
                     /gene="fecB"
                     /locus_tag="J1138_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBA8453187.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(26719..29043)
                     /gene="fecA"
                     /locus_tag="J1138_001366"
     CDS             complement(26719..29043)
                     /gene="fecA"
                     /locus_tag="J1138_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBA8453188.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGITLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(29130..30083)
                     /gene="fecR"
                     /locus_tag="J1138_001367"
     CDS             complement(29130..30083)
                     /gene="fecR"
                     /locus_tag="J1138_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBA8453189.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQHHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(30080..30600)
                     /locus_tag="J1138_001368"
                     /pseudo
     CDS             complement(30080..30600)
                     /locus_tag="J1138_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            30892..31589
                     /locus_tag="J1138_001369"
                     /pseudo
     CDS             30892..31589
                     /locus_tag="J1138_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(31749..31880)
                     /locus_tag="J1138_001370"
                     /pseudo
     CDS             complement(31749..31880)
                     /locus_tag="J1138_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32162..33520
                     /locus_tag="J1138_001371"
     CDS             32162..33520
                     /locus_tag="J1138_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBA8453190.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAESIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(33759..35154)
                     /locus_tag="J1138_001372"
                     /pseudo
     CDS             complement(33759..35154)
                     /locus_tag="J1138_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(35204..35551)
                     /gene="tnpB"
                     /locus_tag="J1138_001373"
     CDS             complement(35204..35551)
                     /gene="tnpB"
                     /locus_tag="J1138_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBA8453191.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(35548..35928)
                     /locus_tag="J1138_001374"
     CDS             complement(35548..35928)
                     /locus_tag="J1138_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA8453192.1"
                     /translation="MQKNVNPGRRKGCPNYPPEFKQQHVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT         8581 a         9287 c        10126 g         8354 t
ORIGIN      
        1 atatcattaa tggtcttgag tatctagttg atgcaaaaaa acaactgctt attataactt
       61 ccaaaaaaat caatggtagg ttaactacat accttttcaa aaccaaccaa aatatggctc
      121 agtggctgag atgcaatatg ctcatgctta atatcagtgt ggcaaaatac attgtgaagg
      181 aatttacgtc aaaacaactc aatgatttaa atgagttatc tcaaaagtta atagaagaac
      241 tcaaggagtt acctgagcgt gaagtgaaaa aaggaattcg tcgtagtccg gaggaagtta
      301 agtctttcat tttagagatt atggagaaaa atcctggtat cagtgctaca cacgcgttgc
      361 gtgaatttcg tgactcggga aattcatttg aggaaaaacg ttttcggact gagtttatgg
      421 ccctgagaga ggctaaacct taatgctact tgcctgggtt tcatggtaga atcccccgtt
      481 ttttgaggct ttgcagtggc gtcacaggag agtaagttgt cgaagctaaa gtattttttc
      541 cctgatagtc aggattttat cgatcccagt ttcgacttct tccgcgaaac gcgtaacgaa
      601 catcgcgtgc gtcaacgtga cgatcagtat ccgcatgagg tttttcctca tccttacgat
      661 ggtatgctgg tttctaaagc tgtagttgat ggtcttggcg gcggcgagag caaatatact
      721 cgtgcccagc gtttgcgtta cttccgtaat ggaatgaagc actttttccg tctgccggaa
      781 aatatggaaa ctatgggtga ttgcggagcc ttcacatatg ttaatcagga cgtcccgcca
      841 taccgtgtgg aagaggtgat tgagttttac gaaacttccc gctttaacca tggcgtttct
      901 ctcgatcata tcgtttttgg ctatgaaaaa cctggtgagg tattcagtgg cgaagtgttg
      961 gctgaatgtc gccgacgcca ggatatcact ctgaccttgg ctcaagagtt tttaaacaag
     1021 tctcaaaagt cctgtttcac tccctttggc gttgcacatg gatggagcaa aacctcctat
     1081 cgtcagtctg tagaggccct gctggccatg ggatacagaa atatcaccat gggggggatg
     1141 gtgccgttga aaacggcgca gatccttgag actctcgaag agattaaacc gctgctgaaa
     1201 agtgatacac gtattcactt gcttggcatt gcccgtccag agagtttcgc cgactttatc
     1261 aagtttggtg tgaccagtat tgactcgaca acaccgctgc agcaagcctt caaagatcgg
     1321 aaaaacaact atcatacgcc tgatggcccg gcctataccg ctgtgcgtgt ccctcagttt
     1381 gatgcgaatc caagtttgag tcgcaaaata aaatctggcg tgattgatca ggatattgca
     1441 cgacatctag aaaaggatgc catggctgcc ctgtttgaat atgataaagg ggctttgtca
     1501 cttgagaaga ctcttgatgc agtcctggca tatgaacgcc tccattcggg tgaaaaagag
     1561 gccgataaaa tccgtgcgga ttatgaacgt acgctaggag aacgtccttg gaaccaatgc
     1621 cagtgcaata tttgccaggc catcggtatc aacgtcatca ttttccgcgg agcagaacgt
     1681 aaccgtcgcc gcggtttcca taatattcag gtgttgtaca accgtttaca gcacacattg
     1741 tcattacgct cagaggaact gtcatgagtg aatatcgtgt tcccgccctg cgaatccgac
     1801 agggtgagga gaggcaactc tatagccttg cgatagaagg taaacagatc agcaaaattg
     1861 cagcgatttc gcggattcgt cgcggagaag agagtttggt tggttatcag cgtccagaag
     1921 tacgtaacca tattcgcgaa attcagcgct atattgagag caaaaacccg atgataccaa
     1981 acccggtgat tatcgctttt gataagcgtg tgcgttttga accgcttaca gataatggcg
     2041 acatggggca tctggtgata cctttctccg aagataagga ttttgagaag ccagggttta
     2101 ttgtggatgg tcaacaacgt actgctgctt tgcgggatgc ggaaatcgac tcgtttatga
     2161 tgccggtctc tgcatttatc gctaatgatg cagaagaaca acgcgagcag tttatgctgg
     2221 ttaactccac aaaacctctg cccaaaacgt tgttatacga actggcaccg catacccacg
     2281 gtcgtttgcc atccgatctg caaatgcgta agtttccttc gctgttggct caacgactga
     2341 acttcgggga aggtccactt gcggggcgca tcaaaacggc aaccaatcct gatggggtta
     2401 ttgctgacaa ctcgatgatc aaaatgatcg atgctagtct tcgtgaggga gcattgtatc
     2461 gcttccgtga ccctgcgaca gggttggggg atgaagggaa gatggtgaaa ctgctgaata
     2521 acttctggtc ggcggtggaa accgtgttta ccgatgactg ggacaaaaag ccgcgctatt
     2581 cccgcctatt acacggcgtc ggtattcttg cgctgggtag cctgatggat gaaatcgatc
     2641 aggtgcatca ggactataaa ggtgaaccag gctggactga gatcccttcc tatactcgtt
     2701 ttgtcgaaga gttaaaccgc atcaaaccgc tttgtgcatg gagtggtggc gtgtggaact
     2761 tcggtacgga tattgatggt cagcctatcg tccgtaaatg gaatgaactg cagaacctgt
     2821 ccaaggatat atcattagta actgattttt tggttatgaa ttatatcaaa tctgtaaacg
     2881 cggatattaa tacctaagtc aaaatgtaag ccctttgtgt aaaacaaagg gtttttgtca
     2941 tttctaggcc aataatttca tattggaact aaatgtcggg tacgattagt agtgtattta
     3001 gctacgctaa aacttagtca aaacccactg attttaatta tttttctata attgtgatcg
     3061 ctgtcactct gcaagataat gatgagaaat aacttatctc ctgattacaa ttgctaaaaa
     3121 atcattccca ccatatatta aattacatgc atacacagcg atattgttcg catcgcaata
     3181 tgatgctatt cgctacagct aataaaaaat taaatgacta cactacccaa tacattctct
     3241 gaaggtgatc tagttacttc tcttttgttt agcggaatcg ccaaaattgt acgtcttcac
     3301 ccgcctggca aggctgagat cgcctggttt gtctcacctc tcgaatacga ggttaaccgc
     3361 cagatggcgg agaccgtatc actgacgccg gcaaaactgt atgaagagag cacggtattc
     3421 ttccgctcac ccgagagcgg actgtggtgt cgggggcgtt atggcgggca acgtccggat
     3481 aataagcatc tggtgatcgt ccgctctgat aagaacgtcg tcatcgatct agctgatatt
     3541 ttttatccaa actacggaca gggactggca atgaacccgg cccatttcct cgccgctcgc
     3601 gccaacgacg cacctttttt ctacccactg cgcgagaagt ttgtaagtgc atggatcaaa
     3661 cagcgcgccg cctgtcggtc aatgtcgtcg ttaatcagca gtcgggtgga gattgagcct
     3721 catcagatag cgattgtccg ccgcattctc caggatcaga accccaaata cctgctagcg
     3781 gacgaagtag gactgggcaa aaccatcgaa gcgggactgg tcatccgaga gcatattctg
     3841 gagtgtaaac gtgaagcccg cgtattggtg gtggtgcctc aggtattgca aggccaatgg
     3901 acgcaggaac tggtcgatcg ttttgcgcta caggaagtga tgagcggagg acgacacacc
     3961 cagattcgtc tgtgccttcc ggaagaactt tgccagccgg aactgatcgg ctggaagccc
     4021 acgatggtgg tgatcgacga ggcccatcaa ctgactcgac aggcatgggc cgatgatgaa
     4081 gatgacctgg cgcagtatgc cgctatggcc gcgttgtgcc atcaggcccg tattgtgctg
     4141 ctcctgtctg gcacaccgat gcacggtaac gagcgtcact tcctggcaat gctgcactgc
     4201 ataaatccac aggcgtggca gcttaatgca cagggaatcg aaaaatttat gcaacgcgtg
     4261 gcagagcgcg agcgtctggg gggaatttac agctctctga cccctgaaac accaaacggg
     4321 gtgctggaag agatcctcga agagttacgt ctgctttttg ctgatgatga gcaactccag
     4381 gcgcgcattg ttggtctgag tccgttggtt gatttttttg cacctgatga aagcgaagag
     4441 cgaacggcgg gcattttggc attacgacag tggataggag agcgctaccg tctttttcac
     4501 cgcttgctgc gtaaccgacg tgaagatccc gcgctggaat acctatttcc aggtctggcc
     4561 gggttggaaa aatgtgtctg gcccgtcgca tcgaccgaga tcacgcttga tgaaaccctt
     4621 gatgcctggc gtgccgcggc ctggcgcaac cccgcacagc atcaacatct ggattccaca
     4681 acgcttgatg aatgggtgga agcgctgttt ctcaacccgc tggtgatgtc ccgccaagcc
     4741 aaaaaggtac tggtaaaagg gaccgccagc gaagaagagt tcaattttct tgagcagatt
     4801 attgaaacgt cccagcagga gcagaaagcc aaagatgtgg cgctaatgaa ggcgctgacc
     4861 gactggtttg aaaaatgtcc tgatggcaaa gcagtcatct tttgcggtga aggatcagaa
     4921 gccgccttcc tgtttaccaa actgcaaatt caggccccct gggcggtagt tcgccatgcg
     4981 ccggaccagc gtaagcagag cgagttgttg gatgacagtt ggcaggtatt gatctgcgat
     5041 aggcgcggcg aagacggact gaatctgcac ggaaacaacc ggcttgcggt ccactatagt
     5101 ctgagccgcg actttaatcg ctttgaacag cggttggggc gctttaaccg ctattccgga
     5161 aatctacgcg gcgttaagcc agtgaaaagc ctagttttac tgcctgagcg tgatgggctg
     5221 cgtgctgact gggtgaaatt actggacgaa ggcacgggat tgtttcatcg aagcgtcgcc
     5281 agcctgcaat ttgttctgtc tgaacaatta gacgtggtat ggcgcgatta cgttgggcag
     5341 gggcttgccg tcttccatga ggcgcaacag cgcttttcgg gagaaaatgg cttcatcgct
     5401 caggagcgta agcgcgttct ggctcaagaa aacttgttat cgatggaaca ggaggtcatt
     5461 gctgcccgcg agttttctga gcaactggcc gaatctgaag atgatgccga agagcaggca
     5521 aaggatatgc tggcttggat gtgcaaggca ttagggttta agcgagaaaa atatcctgaa
     5581 ggcggatttc gcctgcgctt cgagcgtggc gaatatcaac ggcaaacgct ggtggacgtg
     5641 ggaactttta ttgataactg cctgctcggt ctggattttt cagagggata tccaccctcc
     5701 acggcgatga tgagcctgtc tcgtacggaa gccggcaacc ataaacatgt ttaccccttg
     5761 cgctacgggc agcctttcgt cgaaaccgta tggcaactga tgcagagcga tccgcgaggt
     5821 gccagtatgg cgctgcttcg ggttctctcc tccgcggttg cgctgaagca accccacacc
     5881 tggttccatt ttcagtggct gagtgaagca caggttgaag gtgaaaatca gctagccgcg
     5941 cagcgtcgtg gtgatgagtg cttcagccca gtggtccaca atttctggct ggatgacggt
     6001 ggaaaagagg ctgacccgca gatcgtcgtc tccctgctgg ataaacctta cgatgaagag
     6061 ggcaaccgtt tgttccagga tatcaatctg cgagaagagg tctggacgcg aatgccggac
     6121 tggtttgatc cgcatagctg gaaagagacc gtactcgctg cggcggagca ggcccggcag
     6181 aacgtgcatg ctcactatgg tgatcggccc gtccgccatc aattgcttgc aatgaaggcg
     6241 attattctgt gtacccgtga catgctgtaa tcggaagcaa caatggaaaa tcagtggcaa
     6301 caacttcgtg ctgttctgaa cggagaactc aacgaacgcg acgggctgag tgggcagttt
     6361 tttaaccgcc tgctttactg cttggatgat gtacgggcgg gagaatgcga ccgcctccac
     6421 gcattttatg atgcactgca atgcgctcgg gcactcgggc tgacgccgtt aaggttgccg
     6481 taccgacacc ttaccgaagg cacaatgctc cagcattttg gtttgagacg ggatcatgac
     6541 tacccaggcg aactgctcct gacgcgcgaa gcggaagaga ttgaccccca tctattaccc
     6601 gtctggaaag gcgagcagcg ccgttttctg gaaaccccac cgctggatgt acttttgccc
     6661 tctgtactca acgatccgcg ttatacgcac tacacgtcgg cagggcaaca gcaggcggta
     6721 cgcacggtgt tgacctgcgg cagggataaa acgctactgg tgaatctgcc caccgggtct
     6781 ggtaaaacct ttgttattca ctcccagatg ttgacttcac cgcgaaggca cctgacgctg
     6841 gtgattgtac caactgtggc gctggcgata gaacaggcgc tccgggcgca agaggtgatg
     6901 caacaggctg ggcaggatca cggtggtcgt tacgtctggc atagtggcca gtcgacagaa
     6961 gaacgtaatg aaatgaagtc gcgactggca tccggcgagc agcgagcgtt gttttgctcc
     7021 cctgaagcag cgacaggtgg gctcttgtta caactgttct cgcttgcgga gcgcggacta
     7081 ctgggcgccg tgatagtgga tgaagcgcac cttatcgatc aatggggggt ggagttccgt
     7141 ccagaatttc agctactggc gccactagtg cggtcgttga aggctacctc gccagacggt
     7201 gtaaaagtcg tgctgctttc tgccactttt agccaaagca ccttgaatac tctcaaagcg
     7261 ctctttgcag gtaaacgcca agggtcagtg attgaagtga acggcagttt tctgcgtccg
     7321 gaaccagtgt ggtatgtcag cgaggctgcc agccacgagg atcatcttgc acaggtggaa
     7381 aacgcaattg ctcgcttgcc ggcgccgatg atcctctaca ccaccgaagt ggaacaggcc
     7441 aaattctggt accagtatct gcgcgagcgt ggttatcggc gctgtggatt gttccatggt
     7501 gcgacgccaa tgcaggaacg agaaggtctt atccacgctt ggcgtaacga tgggctggat
     7561 atcatggtgg cgacttcggc atttggtgtt gggatggata aaaacaacgt ccgttccgtc
     7621 ttgcatgtcg cagttcctga aaatttagat cgtttctacc aagagagcgg tcgtggcggt
     7681 cgtgatggta aagcatcggt cgcgcacatt attttccatc acaaacagct acaggtcgca
     7741 cggaacatta accgtaccaa actgatcggt gccagcaaag gatatttgcg ctgggccaaa
     7801 atgcaccagt tgcgcgagca gcatcagccg ggtcagttca tcgttccgct acgcgcgaag
     7861 catgctgaca ttcgcatgga ttcgcagagc aatgtggact ggaacctgcg taccttactg
     7921 ttaatgcagc gggccgggtt tatcgatatc gcctatccgc ctccggatct tagcgctatc
     7981 gccccggacg agcgtgatga agcaaagatc cgggcgtggt ttgaccatta tttcaaccat
     8041 attcaggttt ccgtacgccg tgatggtcat atggatgaag cgcggtggca tgagggcatt
     8101 caggcccacc gttcgcatga actggggatg cgaaaacagg gatatagcgc gttggaggag
     8161 tggctcaatg acccttcaat ctcgttgtgc cagaagttag cgcaatttta caccttggat
     8221 ggctttgtgc cggaaatagc ctgcggcgga tgcccagcct gtcggagtaa agggtatcct
     8281 ccctttactc cgacgctcgg tcgtatcgcg cacgtgacgg gcgaggcggt gcggaacgtt
     8341 atgggaaatg agcaacgcgt ttattatcct gcgacgctga cgaaccggct actgcttcgt
     8401 caatggagtg actggattgc ccgactccta gccaatcgcc agattcaggc tatccgcgcc
     8461 agccagagcg tactcgccca acttagcgcg gtactgcccg caggtttgcc tttctggtgt
     8521 tcgcttgccg ttgatgaaga aaacacctgc tgggacgaac tggtgctggt gttgcccgac
     8581 gaagcgatgc cggaactgga cattttcgcc accatcaatc gcattattgt tgcgccggaa
     8641 cgcttacagg aaccgggcta ccgcgggcgt cgctggtggg aggttgatac cagtgccgtg
     8701 gcattagaac aatttcagag gaatattatc tagtggcaat taccaataac gctgccgcag
     8761 ggagccagat ccgtctgctg tgcatgatcg accgggtact gaatcggcgg accggacagg
     8821 cgatcccgat ggcagatttg attgaattac tgcgcccgga gatgctgccc gtcagcgatg
     8881 gcgcgagaaa acgtctgccc gccgaagtct ctttctggac aaaagaaggg ttgtggaaaa
     8941 atgagagcgc tggcctgagt caacagtcgc cgctttccag cgaacgtaat ctgcccgcac
     9001 gggttttgcg tacgctaatt aacagtgaga aaacgacgcc gctgctgact ggaacgcgcg
     9061 gtcagccctt gctgatgtgt gtcacctcca tgcttgcaca ggaaaaatat acgttacgtg
     9121 gaagcacgcc gctgacaaaa gaggcggtgc cgctggccgt tggccccatg attaataata
     9181 aattagcagg tgaggggcga cgtaacctca acgtctctaa cgaagcagaa cccttcctga
     9241 attacgcgta ttttctcggt tttacagaac cttatctgaa tggctgggtg atggacccta
     9301 cccgcgccat tgaaggaatt ttggataacc tggggcttac cgccgggatg ccaattcagg
     9361 agttcctcag ccaactggcg gagtttctgc caatgctgga tgggggagcc taccgtcagc
     9421 aggtagaacc gatgatctct gctgacaact ggcaaccgct ggagaaacat atgattagtg
     9481 cttctttgag ccaggcgttg cttcgtcttg agctgactat gcagttggtt ttcagttcgc
     9541 gttctgatga cctggacgcc atggtcctgc aaggtccgga tggcagcctg cgtcgtatca
     9601 gtaccgttag ccctggggag gcaagaaaat gagaatgatc gaatactggc cttccttgaa
     9661 aaacatcaat gaatgtattc gcaccgaggc ggaagagttg gcggagtaca ccttactggc
     9721 cgtgcatcaa ccggtgaatt tgctgcgtcg tgataaagac ggcaacaacc tcggatatgc
     9781 gaaagaagag gatctgctca accactttct ggaaacgccg cgcccaatcc cggtagtggg
     9841 taaagctggg gtggggaaat cgcacatcat ccgctggttg gatgcgaaac tgcgattgcg
     9901 ccaggagtac aaagacaaga agtggcacat tgttcgtatt cccaaaagtg ccagcctgcg
     9961 tgaagttctc acctccatgc tggctggcct ggaaggcgag atcttcgacg aggcgcggga
    10021 agacatcaat aaggtgtcgg ataaacgcac tcctcgcgag attgctgaat ggctactgat
    10081 gctgatgggg caagagctgc gcaacctgca tgaacgcaac aagcaggata tggaagcact
    10141 gaaaaaacag atggcgggga gtactcctga gcagttaaaa gccatgaagc ctcaggcgac
    10201 acggctccag aaaatccaca tccacgctga agataatgca ttgccaacgc tgattaacga
    10261 tgcctacttc aagcagtttt tgttgaaaga agagcactgc ctctatcgtc ttgccagccg
    10321 tctgaccaac ggcgcaacca gcagcgatct cgacgaaggg gaacagcaac ttcaggccaa
    10381 agaccttgat ttcagcttta acatcaatga cctcagtctg ccggcgcgtc agtatattca
    10441 gaaaagccgc cttaatacgc atgaggacgg gcgcaaagat gcctccgaga tcctcaacct
    10501 ggtgctgggt aaagcgacgc aggcgctgtt taaccagctc ttcaacttcc gtggccgcag
    10561 tttctccgac ctgtttactc agattcgtca ggcgctacat gaaaagcata tgacgctgat
    10621 ggtgctggta gaggatatgt cgctcatcac cgcgatcgaa gatgtgctga tcgacagcct
    10681 ggaaagggaa gggatccgag acgggaaaga ggtgctgtgc cctgtgtgtt cagccattgc
    10741 cgttactgac ggttatcagg ggtatgcccg ccgccgtcag gggatgctgg atcgcgcgaa
    10801 gggtgaatgg gtgatcgaag aggtaggaag cgggcgcgaa gagacgcgac tgcgtattgt
    10861 cgatttttgt ggtcgataca tcaacgcggc ccgctttggg agcgaagcct tattacagcg
    10921 ctgggagcaa gccgccgata aatctcactg gccacctgac tgggaagcca gcgcaaacgg
    10981 tgacgaaatg gccgaagtgt ttgggcgaag cgagcaaggt ttttctttgt atcccttcaa
    11041 cgctagcgct atccatgcgt tggcagaggc attctgccgc gacgatcgca acgaattaaa
    11101 attcaaccca cgtcagatca tcaaccagat actgttgcgt gtactacagc actgtcgccg
    11161 tgatgccgat gagggacgct ttccacctcc gcgtcttggc gatattgctg ctccggcagg
    11221 gcttcgaagc tggctgttcc gccagggctt tgccgatacc gagcgtgccg aatccgtggt
    11281 cgcgctgtgg ggttatcccg ccgattccga cgccactttg gccagtacgc tgccgcctga
    11341 cgttgcgcgc agtttcgatc tggaagatct ggcgcaggtg ctggaaaaca caaaaagtgc
    11401 cccgctagtt gaacaatcgg tgatgccaac gcgggtgacc aaagttgagc agcaggttaa
    11461 aaaaaccgtg cagccaccta aaccagtgaa acccgtgctc aaggaggata aagatacctt
    11521 ggctatccgc gcgctggatg cagcggtggg cgactggatg ctaaaaggcg cgccgcttga
    11581 gatcgatcct gcacgatata tcaaaagctc cctggccttt ttctttgata agcgcgccgt
    11641 ggcggaatgg gcgggcagca gctatcggcc gaccctttgg ttgggtaaat ccaactttgt
    11701 tgccgttgag ttacctaacg cgcagggtaa ccgtggcgta catgtggtga aatttatttc
    11761 ccagtccgaa tacgacaagc gctcggtgca attaacggat gccgcgatgg cgctggcgcg
    11821 gtttggctac tacagagaaa actccttaac taagaccgag gactggagct acccggaggg
    11881 caaaacggat tacctgatta ttcagagctt ctgcgatcgc tgggttaact atgcgctgac
    11941 ggaactcgtt aagcataagc gcaacgatct gccactgctt ctcagcgagc agattgcact
    12001 tgccgatgcg ctgggggtga ttaaaacggc cgatggtccg aaagaggtgc ttgggcgact
    12061 gctgcaaaac agcaaaacgc tttcttcaca gttccgcacc gggatcacga aggcaattac
    12121 cgaactgcga ggagaggcgt tagcaaagtg ggatgatgcc caggacgcat ggctcagcct
    12181 ggtggcgcta aacgatcatg cgctggaagg cgatctcctg ctgagtgcga tacaaaaggt
    12241 gttaaaaaaa cgctcaaata acacacacgc agccgtggtg aaaaagtcgc tcagtgaaat
    12301 ccgccccgca ctggatactg ctgctctgtt tgccgactgt gaaaatgcag atgatttttc
    12361 cgaactcgtc accggcctgg ctcagttggt taaatccctt ggggatagcg gtgattatcc
    12421 tgcggatatg acacccgaca gtagtacgct tgcgaactct ctgactgggt taacggaagg
    12481 tggagtctgg ttgacgatcc tcaagttgcg aggcattacg caatctgaag atccgcttcg
    12541 ccaatggcag ttactttgcg aactggacgg tactttaatt aaccgactca tattcacaat
    12601 cagtggctgg cagcaggtca acaagcgggt gctagcgaat attaccgcct acaaccattc
    12661 gcatggcagt caccagatca gcgaatttcg cacgcagatt gaaagcacgt tgcaggaact
    12721 tcatcaggtc ctcgacgcca tgcagtccgt cgcaggggaa cagtatgaca acgcttaaga
    12781 cccaacttac gcaactgcgg gagcagtatc agataaaaca gcaggttcag tcgctggaag
    12841 aggtctacga tcggtaccgc accattcgtg acggcttgct ggatgttgcc gaaccgctgc
    12901 aaaaagccca aaagcaactt gaagtgatcg gaacgctacc ggaaccacac tccgctatcg
    12961 atttttcgca agaggcggcg cttttcggcg gtgccaaaac gcagttggat gcgctgactg
    13021 cgcgcttcaa agaaagtggc gataaaaccg agcagtgcgg gttttgggaa acggtgcggg
    13081 ttctgaagag cgtacatgaa agtattgatg ataaggtgga tgcaacctgg aaagcgtttg
    13141 ttgccgatct tgaagcgcgc gcaacgctgg atcccgtctt tctggaacaa cagcggacgc
    13201 tggggaacgt gttggtgtat gacgattatc gcaaagcgcg cgataacttt aatcggcaga
    13261 agttaagcgg gccagacagc ctgtcggtgg taaaaaacct gcagaagtat agcgatgaga
    13321 tgatcgcgct taaacgttca atggacctgg atgcaccaga cgatgttctg cgcttttttg
    13381 aggcgctgga cagacataat catgcctcgc ttacgctgct gacgccgggc gtgattacct
    13441 ggctggaaga aaaagatatg ctgccagcgt ttaacgtgac acgtaagaga atgatttaat
    13501 gaagggaatc gatctttcgc ttttgcaacg cgtgctagat gcactggaag aacaagaaaa
    13561 tgcgctcctg gtatggggtg acacgggcgg cttttttaat ctggatgaac ttttaagcgt
    13621 tatcggacgg gttttacctg acgaagatgc cgaagtggtt ctggatcaaa tgctagaggc
    13681 cgctatgctg atccaggtgc cgcacgtcag tggcacgcct gtctggcgaa cccgaatggg
    13741 ggagacggtc catctcatgc gcaacctgcg ccagtggatg cataagcaaa aactggacca
    13801 gagcaaaacg ctggtatcgg actatcgctt tattcgccgg ccgcgcagct atcccgaccg
    13861 cgtttacgaa ccggcaacgc tgattagcgg ctggaaaaaa cagctgaaat taagcgatcg
    13921 cgtttgtgac atcatgcgcc aggcgctggc gtcagataag cccttccttc tggcgggttt
    13981 tcaggtgcgc gctaccgaac gcattatgca gtgctggcag gatcatcagg tgaaaagcaa
    14041 cacggccagc gggaccattg tctgtgctgg caccggtagc ggtaaaacgc tgtcgtttta
    14101 cctgcctgcg ctaacccgtc tcgctgagga tatttgcaac aaccctgagc gcaaggtgcg
    14161 cattctcgcc atctatccgc gaaaagagtt gctcaaggac cagtttgccg aaactttttc
    14221 acagtgccgc aagcttgacg actatatgct cacggcggcc ggaagaaaga ttcgtatcgg
    14281 tgcctttttt ggcgatacac cggtgaaggc ggaatggtcg aaggaagacg ttaaggggaa
    14341 ggttggtctg ccgttcgggt tgatgaaatg ccagcagccc cacccgcatc atcctaagca
    14401 gaggtgcgga ggggctctca tttggcgtcg tgaggacgta tttggcgagc gtgaggtact
    14461 tacctgtacg cagtgccagc atcaggtgga tcagagtgaa atcatgatca cccgtgatgc
    14521 gcagcaacat cgcggcgacg cgccggacat tctgtttacc acaacggaaa tgttgaacct
    14581 ccagatgaac tccacctgga gcaaccacct gtttggcgtc ggcgagggct atgggccgac
    14641 gctggttctg ctcgatgagg tccacaccta cagcggaacc accggcgcgc aaactgcgtt
    14701 tctgctgcgg cgctggatgc agcgcaccga ttgtttgccg cattttgttg gcctgtctgc
    14761 aacgcttacc gacgcgcagc acttctttgc caggttggtg ggtgccgctg aggagcaggt
    14821 tgcgctgatc cagccctaca tggaagatat gatcgaagag ggggccgagt acctgctggc
    14881 gctgcgcggc gatccggttt ctgaaacggc attactctcc accaccatcc aggccagtat
    14941 gctaatggcg cgcatgctgg atagcaaagc gaataagtcg caaggtacgt ggggcaagaa
    15001 gaccttcatc ttcaccgaca ctctcgacgg taacaaccgt ttgtaccatg acctcagcga
    15061 tgccgaaggt tgggagacgg gcccagggca ctcgcgcatt gatcactcac cgttggctgt
    15121 gttacgtagc ccttttgata atactgcacc tgagcggtct aaaaccgaac tggggcaaaa
    15181 ctggagggcg gccacggata ttggccacga cctcgcggaa aacaaagtca tttcccgaac
    15241 gtcgagccag gatgccggcg ttgatgcatc agcggatgtc gtggtcgcca cctcttcgct
    15301 cgaggtgggg tataacgatc cgctggtcgg tgccgttctt cagcataagg cgccgaacga
    15361 tgtcgcctcc tatttgcagc gtaaaggtcg tgccggacgt ccacgaggca tgcgtccctg
    15421 gatgctggtg gtgctctccg agtttggccg cgatcgcgtc gagttccagc gttatgaagg
    15481 gctaatgagc ccggagatca aacgtcaggg tctgccgctg gacaaccagc acgtacagaa
    15541 aatgcaggca gcaatggcaa ccctcgactg gatcagcaaa gtcggtcagt tcaaagatct
    15601 ctggagcatg ttgaaaaaag ctgagcataa tcagctgaaa tacaatcgaa tgtatggccc
    15661 gcttatcaaa ctgattgaag aggtgctgag cggcggcagg cggttgaacg aattgatgcg
    15721 ctaccttcag gatgctctgc aattatctga tggcgcggtg cagaatatcc tctggagccc
    15781 gccgcgctcg atcatgtttg agtttctacc aaccatactg cgtaacctca gaacgcgctg
    15841 gtcggttaac ggcgtggagt gggccggact gcgtccgaat cagccaaata gcgagggcga
    15901 acagcaccgg agcaattcac ccgtcccgga atacatcccg cagaacctgt ttagcgagct
    15961 aaacttgccg gaactggaca ttcgcctgaa gcgtggattt gacgatgaag accactggga
    16021 aactctctct ttctggcagg gaatacgtga atttgcgcct gggcgactct ctaaacgcta
    16081 tgccgtcaaa tcgaacaaaa gtaccgactg gctggtgcca caatcctacg agccgatggc
    16141 aggggaaggg cggcaatttg ttgatttcca gataagcgat gcgttcggcg atagctggca
    16201 aaacgagtat gaagttgact atatgggaaa aaccatcaag gttgttaaac cgtcgaaggt
    16261 catgaccact cgggccgaca ttcgccggat caatgataaa agtaatgcgc aactgcaatg
    16321 ggtgttcaac gtcattaacc ccgctattgc tacgccagac gaggtgccga agggaccctg
    16381 gaagcacacg ctgagcgatg tcacgttcta caaccaccag catatgaccc cgctggaact
    16441 ggtccgcttt tctactggat cgcaggcgtc gctccggttc aggaataagg aacgggctca
    16501 cgttgatttc acctgggtta atggcgagga acaggttggc ataggaagtc gtcaatgggt
    16561 ggatgcaatg cgcctgcgtt ttaacctcac ctgtgatgat gtgcttggtc tgctacatca
    16621 agaagaaatc cagcgaggca tgcgccccgt ttattttcaa catcttgtaa gacagtcgcc
    16681 ggaatttgaa tttgacagtt tcaacgcgga ctgggccatt gagtgtttta tggctcagct
    16741 tgcggagaca ctggcaaacg gcgctcacgc ctcggttgag tcggcattac gagaaatggc
    16801 ctccgaaaaa gggggagaaa ggctggcaga tattccggcc agcctgttcc agccggatac
    16861 cgacaatgaa acaggcactg atcaggcgct acagattggc ctcaacaaac ttcttcagcg
    16921 accggaaata cagcagttgc tgctgaactg cgcgcaggcg ctctggaaac ccctggatga
    16981 aatcgacggt tttgttgaat gggcacgcca ggtattagcg gacaccctgg ctgcgggtgt
    17041 gcagcaaacg ctaagcacgc tgcttccgga cgttgatgag cgagcggtag tgacggattc
    17101 cagttggatg agcgatcccc gcaagggagc agaatggctg gaaatctggc tctgtgagat
    17161 ggagtctggc ggcagcggta ttctgattcg tttacagcag aagtgggctg aagatcctgt
    17221 ctccttcctc aatgtactag tgcgtaatct ttcagccagc gactacgagc agatcgatta
    17281 cgatctgcga actgtgttgc agatgatgca aacggatgac acgcttcgta tggcgatctc
    17341 agccgtacgt gaagccagca acatggacgc ccggcgggag gctaacaaga atctgcattt
    17401 gcagctttca cagcgcggta tgcgcctttc ccacagcttt accacggtgc tgtactcgcg
    17461 gattctgcgg gcgggcagcg gcgagcacac agatacccag ctttatcagc tactgtcgga
    17521 ctggtcgtcg cttgaagcga gcgcgggtat tgaattcagt atgaacacca tggcgcacgc
    17581 gttggcggtg aactcgcttg gcgtagaaac tgatgcttcc gtcgtgtttg acgagcagtg
    17641 ccggaatcaa aacctgctct ggccacgagg ctatacgatc cgacaggctg agctcggctt
    17701 ttacaatatg ttttgctcgc gcaaagtcac aaccgagcgt ctgcttgctg gcgcgttatt
    17761 tagcgagcaa atcgaaaaga tcgcccttga cgatgactgg cttgggcatt tgcatgcggc
    17821 gctgcgtaaa ggcggtcgtg cggaactgat cctcactcgc cagcagcgaa atcaattaca
    17881 tcaggtcatt accaccgtgc aaattgaacc cgtcgatcat ttgggactgc tgctgtatcc
    17941 gcgtctcggt gaagtccgcc gtgagcagga tgtgctcata ttgcgtattg aactggcaga
    18001 ggcgatgcaa tgatcggacg tgcaaaacag cggcagattt ttttacatgg ctccgcaggg
    18061 caccggcagt tgcgagaagt cctcagcgcc caggttgttg ctctgattat gcagccagag
    18121 ccaatctggc tggtttcgcc gtgggtgagt aacttctcac tgctggacaa tcgctcaggc
    18181 aattgggaca gcattgatcc cgcgtgggga ggccgggagg tggatttcat tgagctgtta
    18241 gcctgtgcgg tgaataacgg cgcgccgctt tcgctggtca cccgcgacga accgatgaac
    18301 cgtatctttg taaaagcgct ggaaaaacgc ctgagtaact ttgcgacctt tcgtttggaa
    18361 tggcgtaacc agttgcatac aaaaggtttt ctgacgccaa atttttttct caagggctcg
    18421 atgaatttta cccggagcgg tgttaatcga aatgaagaat ttgtcgaact gaactgcgat
    18481 tcgcatatga tcggtgaagc gatggccgaa tttgaaagtc agtatctttt tattggatca
    18541 aaggaagcgt tctgatgtgt gcagactggc tgaaaaatta ttacgctgaa gataactatc
    18601 tggattatga caaggcgatg cagggggggt atggaatccc acaaatgaat acgctgattc
    18661 aacaggccgc agcgctacgg atgccctgta tcgttccttc aacgcgaacg ggaagaacgg
    18721 tgttttatgc tctggctgag aatgcgaaaa gcctagacga attacgacgt attttaaccg
    18781 cggcgttggg ttccgctgac actactccgg atattaaatc actctatcat tctgacgatg
    18841 gcggggagca gttgctgctg gagaaatccc cggacggaat tctggccttc gatttcctac
    18901 cggtgccgga cggttcgccg caacaggtaa aagaatggca ggcagcacga atgaaacggg
    18961 tttatgccat gctgcaaacg gtgatggatc tttatcatca gcgacctgtc ttacattcgc
    19021 tggtcagtcg gcaaaccgga cgaattctca gggatttcta cacagcatgc caggctcgcg
    19081 atggcaagat tgccgaacag tatctggaag agttacgcgg aaatcagacg ctttcttctc
    19141 tgaatctcct gtttcttgag ctacagggga tggcggcttc agcaaaatgg gacgcgattc
    19201 tcaaccaccc tcggctggaa gtattattgc gcggccgggt gccggagcgt atccagcgcc
    19261 tgctgttgcg atcctctggc catttaatgc ttaacgccat ccgcgatgcg cacttcccgc
    19321 tggacaggcg tgaagatgcc aggcgactgg tgctgggctt attgccgctg tacaagcata
    19381 agccccgctt tgctcatcag gcgagtttcc tgcctgactg gcaactgtgg gccatggggg
    19441 ccgcgctgct ggggatcgac gaatggcaaa cggcgacgcc tctattagct cctgactgga
    19501 tacagcaggt cgaggggtgg gcgacaggca caacctcgct gcccgcgccg gtagcagcgg
    19561 aagaacaggc gctgatccag gcgccggtta tcatgctgat aagccttgaa aacgcgaccg
    19621 atttattact cgaagccctt ctggcggacg ctgagcgcga aagtgagatc tacgcgcaac
    19681 tggcggcgat gcctgaagca acacgacagg cattgcaaaa aatacccaaa ctctgggagg
    19741 catggcaggc gctgaaaaaa cgctgtgagc ctcaggacta tggctggtgc cgctggcttg
    19801 cagatttaca acagactacg gaatccgaac ggtttgaatc attgcgccag caggcgaccg
    19861 ttcactacat ggactgggca ccgtcaacct tcagcgaaac gcagtggcag gcgctgcttg
    19921 agcatcagtc caacacccag ttgagtaagg ttttgcgaga cgtactgcca actcttctga
    19981 actggttgga agaatatgac gttaaggtat ctgcctcctt gtggccagac tggcttatgc
    20041 tgctggcagt tgaggacata cgctcagaag aagatgtacg tctcggcggg atgattctgg
    20101 ataaattcct ttccggtccc tttacctgcg aagaatattc ggcagcgatt gaatcgacag
    20161 agattctatg tgctgaaaac gtgtcggtga ggacgttggg ttattctctt gatattgctg
    20221 aacttctgta tgacagggtc actgcaaacg aagcctccag attaggtttc tggattaagt
    20281 tgcaggagtt actcaagcat cgctgggagc gcctggatgc cagtatgcag ttttctgcac
    20341 gcatggtgga aaggctctat ctcggcgaac atgccgggaa cgttttcccg tcggaggaca
    20401 atacgccggg cgtagcatct tcgcttcatc gcgatctcag cggtaaaacc ttagcgattt
    20461 acagcctgat gaaaggggcg gcaagacgcg gaaaagaggc gttgcttcag ctttatccag
    20521 gactggaagt agagctcaac cacgatcatg tcgctacccc tgcgctggtg aatctggcga
    20581 aaacagcgga ctactttatc ttcgctagta gcagttcaaa gcatcaggcg ttctatgctg
    20641 tgacggatta tcgcaaagag atcatctacc cctctggaaa gggggcgtcc agtatgatcg
    20701 catctttcgt cagggccatg gagtaaataa ggaatagcct ctgctattcc ttatttgttg
    20761 tcacctctga accacctcct atggaggtgg tgattgttcc tgtagggcta taacccgaag
    20821 aaagcccatg cggaaatgtt tcaccttata cccggatgct tcagagaagc agtgcggttt
    20881 ttctcggtaa tgcaaccaga aacaatcctg aagaacgggt aataaatgtt ttaagtctcc
    20941 ttttagggga ccgcatcaaa gccaccttct aagaaggtgg attctttact attcaccacg
    21001 gtaaatgcag cctgcggagc atgtctcttt cactatcacc gcgctcagga tagatagctg
    21061 aggcttaatc tgattccaaa tccattcagc aagcacttcg ctggtagggt tctccagtcc
    21121 cgttatatca ttgagatagt agtgatccag tctatccaga gtgggtttaa aaatcgcttt
    21181 tagttcggca aagtcgataa tccaaccggt atgcttatcc acttcacctg ttatctcaag
    21241 gcgcacgaca aaagagtgtc cgtgtaagcg tccgcattta tggccattcg gcacattggg
    21301 aagacggtgc gcggcttcaa aggtgaaatc tttgtataac gttgttttca tagggacctc
    21361 attttttgtt ataccgtaac aataaggggc gatagttcct agcatttatg agttaacttt
    21421 taacattcta ttgggtaggc atgaatattt aggtcgagtt tcaactagac tgttttttac
    21481 tgtgggacgt catgtaggat gagcatacac ctaaattgtc cgttatcgct ggtatcagtt
    21541 gcacaacgac cacctcactg acatcgacga agattggtga caagaaagca tttcagcaac
    21601 ctcatgatcc catctttgtt agctggctga agtctccggt acggcaggca gagcaatagc
    21661 attcaccata gtaatttttc ccgcattgta tacagttcca gtcggcttta tatacccttg
    21721 gtgttgcatc gggaaggaag cgttgcaggc gtcttgcgag gtgtatttca ctcgcgtctg
    21781 gctttacgta ggggcaattg tgctgaccat tttcggcgga acccgcatca atgtgttcaa
    21841 accacgggcg ttcagtgtcg tattcaggat ggtatttcag tgcactgtga cagaggtggc
    21901 aggtatagcg gtcgtaaggg gcggtctgtg ccgttctggc accggtaaga cgatcgttac
    21961 cgtcgagagc gataaaagat ttagcaaaca taatattact cccgccagac aacacgcagg
    22021 cgtaagccgt ccctgaaagg cggacagacg ccatgtggtt gaatggttta aaaggattga
    22081 gaagggaatt gctgtgtggt tgtcacacta tgggatgttg gcgtaaataa atcgccacca
    22141 gcaaaatgaa gtggcacact gaatttggcc acctgaaggg agccgcggtc gcctagcagt
    22201 tgcagtagca ggagatcatc cagccgcagt acaggtcacg atgactctgg ttaatgatac
    22261 cggctttgac cccgtatttt ccggctctat cgctgaatca tggcgtcagc agccgtgcac
    22321 accatcctat tgttgtgact gggaggctgc caccatgctt cgcgctttcc ctctggcgaa
    22381 aaagggagaa ggacgggccc gtctgccttc actttatgcc agcttcggta agctgggtga
    22441 gacaccgact catgaagata tcattgataa caatcgatcc atcaactggc ctgtataacg
    22501 tggctgccgg tgattaagaa agctgcacct acctaagtag tagcaaacgc acacttttta
    22561 gaaaaatcga tggtcagaaa ctggattagc aattccgttc cagggttgct tttgatttac
    22621 gttggcgtct gatcattgat ttatcctcaa aagcccaacc tcattggtaa tgaaccagct
    22681 ccgtgaatgt ccgctctggc acagagcgaa attttttgat ctccccccct gaaatctaaa
    22741 cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt cgttgccgta
    22801 agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgcagtg atcacgactg
    22861 gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg aacctgtcag
    22921 cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg aaaagttcag
    22981 ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg gtttggttct
    23041 tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg gctcggggtg
    23101 tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca cctcttctgg
    23161 tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc agtaccggct
    23221 agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc ggagtttgcc
    23281 catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg gctcatcaag
    23341 taatacaacg ggcgtattct gggccaggac catcgccaga aatgcgcgct ggcgctgacc
    23401 gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct ggttcatggc
    23461 gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca gccagggatt
    23521 acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat ggtgctgagg
    23581 cagcagcgaa agcctgcggg ccaactggcg tgatgagagc atatttatgg gattatcgcc
    23641 gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta acagcgtcga
    23701 ttttccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca gtgagagtga
    23761 aacgtcgtta agtaccttgt ctgtcccgta actgaccgtc agattttcag ttcgtaaagt
    23821 catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa tggcggtcag
    23881 catgccaacc gggagctcca gtgggggatg aataattctc gccagcagat cggcaaccac
    23941 caacagcaac gcacctgtca gggccgaaac aggcagcagc ctgcggtgac gtccaccggt
    24001 gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc cgcaggcggc
    24061 cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg tatggggcac
    24121 cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga gatcgcggca
    24181 aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct tcacaaagct
    24241 ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca catcctgcgg
    24301 gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg ccacgccggt
    24361 gagcgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata tcaaccccgc
    24421 catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg gcataagaag
    24481 tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat ccggtgatgc
    24541 cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg ctgcaccgac
    24601 aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat aatgctcgcg
    24661 tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc ccatatggag
    24721 tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg caattttcat
    24781 cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag cactgcgcct
    24841 gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag cagcatcagc
    24901 gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc tgcccagaag
    24961 cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac gctgacgcac
    25021 gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt caggttcact
    25081 cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt cgccagcagc
    25141 aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc ctgccagcgg
    25201 gcgtgggaca ccccgcctgc cagccagtaa aagatgccgt aagcatgatc ttcggccagc
    25261 agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc cgcgaggatc
    25321 agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac cagcagccaa
    25381 ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat cggcgtcgga
    25441 ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc gagcagtgaa
    25501 ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag cgccaggctt
    25561 gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac cagcgcttct
    25621 ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc ggaaacagga
    25681 atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc aacgggaagc
    25741 ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc ggctgatggt
    25801 ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg cgcatccgcg
    25861 cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt aacatctgcc
    25921 agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg gcaaccagca
    25981 gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg gacgcacccg
    26041 ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc caggtctcct
    26101 gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt gtccctttgg
    26161 gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt gcctgcatct
    26221 ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt tcagcgtagg
    26281 tttcgttgcg ggacttaagc agcagtaccg gcgcgatttg ctgcaaggcg atgtaaaccc
    26341 ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca atggcttcca
    26401 ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca cgcacttcgg
    26461 gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg tccacggcgg
    26521 ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt ttttcgagtg
    26581 taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc accagcagca
    26641 ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta aaagcccggc
    26701 aagccgggcg ttaacacatc agaacttcaa cgacccctgc atatacagcg tgcgcggctg
    26761 gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt cctggtcgaa
    26821 gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa agtcatacgc
    26881 cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt cggcgctctc
    26941 tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt tcagattgaa
    27001 cgtccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg gggagaatgg
    27061 taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat acgcatagct
    27121 ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt aacgtgcctg
    27181 cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt tggtctggtt
    27241 ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg tcagcgcgcc
    27301 gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca cattgccgct
    27361 ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt cagtgtttgc
    27421 ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa gcggtgcgtt
    27481 atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact cgatatgttc
    27541 gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat ccagatacca
    27601 ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc cggacggcaa
    27661 ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat tcaaatagcg
    27721 atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc tgtagcgtgg
    27781 ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc cttgctccag
    27841 gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat gctggctgtc
    27901 tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc ggtcatacgg
    27961 gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac cgggcatgtc
    28021 ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat ccggcgcata
    28081 tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc agtcactacc
    28141 gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc cgcccaccat
    28201 caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct gaccttccac
    28261 gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca ccacgccgcc
    28321 cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa tggcatccat
    28381 gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg cgaaggggac
    28441 ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca ggccccggat
    28501 gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg cgctgacgcc
    28561 agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga aatcctcacg
    28621 gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg catcacccag
    28681 ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct ccagcgtcca
    28741 gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt gttgcaggcc
    28801 gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg tcaggctggc
    28861 gtcaaccgag agggtaattc cgctgtgtgc ggcatactga ttgagcgctt tgtcgagcga
    28921 tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg ccagcggcag
    28981 caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc gtaacggcgt
    29041 cataccttct ccatcatcat ttttgttgtg ttcagctatg agtcgaacga gaagggtaaa
    29101 aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat ccagtaccgc
    29161 gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat cgcatcggta
    29221 tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc gcagcgcagc
    29281 acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt atcgctgaag
    29341 ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac tgcgccaaac
    29401 tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg cgcgggggca
    29461 ctggcgagaa gcacttccac ggcgtgatgc tgcacgtcaa gctgcgtgaa attatcctgc
    29521 tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt caggacgcgg
    29581 aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta ccagagccgg
    29641 acggtgcgct gatgcgcatc aaaacgcaca tccgccgcgc tttgggtatt cagtgtgagc
    29701 agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg gtaatctgcc
    29761 cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc gccgagcaac
    29821 agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa cgcccggctg
    29881 gcaacgtcgc caggcacacc tccaagctgg ttgcgcaggt tttcaacctg ctgccaggcc
    29941 cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg ttgtgggctg
    30001 acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc ctgacggcgg
    30061 gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg cagtgctcga
    30121 cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg tgcgcaatct
    30181 cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt ttgccgttta
    30241 gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg cgttcctcag
    30301 gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct ttttccagcg
    30361 cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcacagg aaggagcgag
    30421 gatcgcggat cgtcgagagc gtttcgctga ccgttacccg caaaaagtgt cctgggcaat
    30481 gtcatctgca tcaaaagcag actggagttt gcgcgtcagc cagcttttca accagccgtg
    30541 atgtgtgcca taaagcgact cgaacgttaa ggaagctgtg gtagtggcgc ggtcagacat
    30601 gcggagtgca tcaaaagtta attatcacgt agccatatta atatgagaat ggttatcatt
    30661 acaattggaa ataaaattgt ttccaataga catttttaac atgttgtttt tctaagtgtt
    30721 ataaggtagg tataaaatgg gatggagcct ctgcttctgg catgtgtcgg tcagaatgac
    30781 tcatgatgtg gtctgctatt attgacatcc tcactgccct aaaggatggg gatttcggta
    30841 atgctgccaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc
    30901 atttccatca gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc
    30961 actgccggac atcagcgcta tctctgctct cattgccgta aaacatggca actacagttc
    31021 acttacaccg cctctcagcc cggtaagcac cagaaaatca ttgatatggc catgaatggc
    31081 gtcggatgtc gcgccagtgc acgcattatg ggcgttggcc tcaacacggt tttacgtcac
    31141 ttaaaaaact caggccgcag tcggtaacct cgcgcataca accgggcagt gatgtgattg
    31201 tctgcgctga aatggacgaa cagtggggct acgtcggtgc taaatcacgt cagcgctggc
    31261 tgttttacgc gtatgacagg atacggagga cggttgtggc gcacgtcttc ggtgaacgca
    31321 ctctggccac actggaacgt cttctgagcc tgctgtcggc ctttgaggtc gtggtataga
    31381 tgacggatgg ctggccgctg tatgaatcac gcctgaaggg aaagttgcac gttatcagca
    31441 agcgttacac tcagcgcatt gagcgacata atctgaatct gagacaacat ctggcaaggc
    31501 tgggacggaa gttactgtcg ttctcaaaat cggtggagtt gcatgacaag gtcatcgggc
    31561 attatctgaa cataaaacac tatcagtaag ttggagtcat tacctcctcc cgaactcaaa
    31621 gctatcatac agataattac tggttaaccc tatctagggc attttaaagt gtgatcttat
    31681 ccagcaataa tggacacgcg gctaagtgaa ccgccccggg tttcctggag agtgttttat
    31741 ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc tttttctgct
    31801 tctgccggtg ggatatggcc ctgcctttcc agcaatcgtc gattgttata ccagtccacc
    31861 cacgtgagtg tggtcagctt atcatactga cacgtgcatt acccctaccg cgataatgca
    31921 accgtacaat atagggcgtt tcctaaacgc agccctcatc actccaatcc aacaagacac
    31981 agcatttttg atcacgaaga aagacaaata atttccgtaa atgtttccgt tgttacaaat
    32041 aacaacagta ttttacattt ctttggcctc aggcccgcgc atgttgccat tcgatgtcat
    32101 tttcctgact cgtttcttct ttatggtgta gtgtgatctt cactttatgg atgctaacaa
    32161 tatgaaaaga aaaattattc ccgtgctaat cggttgtacg ctctctttct ctgccctggc
    32221 ggcacaacct acggctgagc gctatgtcgt cagttttcca gaaggcacgc acgtgaacta
    32281 cgccggcgca tttgccagtg cattcccgaa cggccttccg gttgggatag gttctggcct
    32341 gctgttcacc ggtaaacagg gcgatgccct gacattcgca accattaccg atcgcggtcc
    32401 gaacgcggat tcaccgaaag aggggaaaaa cgaaactaaa attttcgtta cgccagattt
    32461 cgcgccgctg ctgatgacga ttcgtgtaca aaacggcaaa gcggagtcca tcgatccccg
    32521 tcctctgcat gacgataaag gcgcgatcaa cggtctgccg ctggcaagcg acgtgattgg
    32581 atccaccaat gaagtggcct tcagcgacac gttacataga ctgaaggggg ataatcgcgg
    32641 gctggatacg gaagggatca ctccggatgg taaaggaggt tactggctgt gcgatgagta
    32701 tggcccgttc ctgattaaca tcgacagtaa agggaaaatc ctggcaatcc acggtccgca
    32761 ggcggcggaa ggggaaaaag cgattgcggg tggtttgcca aatatcctca aatggcgtca
    32821 ggcgaaccgc ggctttgaag gcctcacccg tatgccggac gggcgtatta ttgtcgccgt
    32881 gcaaagtacg ctggatatcg acgctaagag caagaaaaag gcgctgttta cgcgtctggt
    32941 gagcttcgat ccggcaagcg gaaaaaccgc gatgtacggt tatcctatcg acagcgcggc
    33001 ctacagcaaa aacagcgacg ctaaaatcgg agacatcgtg gcgctcgata accagcacat
    33061 cctgctgatc gaacagggaa gagataaaaa caacagaatg cgtaacctga tttacgaggt
    33121 cgatctcaat aaggcgagcg acctgtcagg cttcgacaag ccgggtgagt atccggagtt
    33181 tgatgatgag aaaaccctgt cacagcgcgg cattaccctt gcgcagaaaa cgcaggttgt
    33241 tgatctgcgc tccttgggct ggcagcagga gaaggctgaa gggttggcgc tgatcgacag
    33301 caaaacgctg gcggtggcga acgataacga ctttggcgtg aaggtggcga tgcaacatcc
    33361 ggtcgagggc aagacattca aggattatcg ggtgaacgcg gaaggaaagc tgacgcttga
    33421 cgacaagcag gttgaaacga ccctcagagt gaagccactg gagaaaccgg agtctgacag
    33481 tgaactgtgg attgtgacgc tgccggaagc gttgaaataa gtttataagg tacggacagc
    33541 agtatcgctt agtttttcaa attcatgtat gtgaacaata ttggggttcc ggcgtagacc
    33601 cccaatgaaa gcgtcaccac gtgaaaacat gcctgcacgg tttacacgtc atcccatggt
    33661 ggcagccggt taactttcct gctacaccag tggaaaaagg ccataagcag tccgaacaaa
    33721 agtaagcgta cagcgagggc cgtattgacg gggatgtgtt attcagctgg cagtgctatg
    33781 cgccacggaa gcagttcgct gacccggttg accggccagt ctgctatgac gccaagcaca
    33841 tggcgaaggt agctttctgg atccacgtca ttcagtttgc acgtcccgat caggctgtac
    33901 agtagcgctc cccgctcacc accatggcca gagccgaaga acagggtctt catcttttca
    33961 cgcaaccagc tttccaggga tttcaacagc ggtttcgttt ttcgctgacg ttcagcaagc
    34021 cgctgctctg ccggcattcc ccttatatcc gcctctatgg cgtacaactg accgatctgc
    34081 tccagggctt cttccgtcag tgctgacggg atgcggacgt gcatatcgtg gatctttcgg
    34141 cgggcatgag cccagcaggc agcttccgtt atcccaccat tgcgatacag ctcgttgaac
    34201 ccggcgtacg catccgcttg cagcacaccg ctgaagcaag caagatgagt ctgcggatgg
    34261 atgccttttc tgtccgggct gtaagcgaac ctcactgcag gtgccaacgc tgacccggca
    34321 ttgcggtcat cacgaacata cgcccacaac cgcccggtct tcgtcttctt attacccggc
    34381 agcagtacct ggaccggggt atcatcggca tggagtttgc cgtcagtcat gacatagcca
    34441 tgaagcgcct cttccagcgg agacagcagc cggcagcatg catccaccca gcccgacagc
    34501 agtgaacggc tcagctccac accttgccgg ccgtatattt ctgactggcg atacagcggg
    34561 gtgtgctctg catacttcga ggtcagcacg cgggccagca gccccggtcc ggcgataccc
    34621 cgctcgatgg gccgcgaagg tgcaggtgcc tgcacgatgg catcgcactg agtacaggca
    34681 tgtttttccc gtaccgtccg gataacccgg aaggcgctac gcatcaactc cagctgttcg
    34741 gcggtatcct cgcccagata gctcagtgaa ccgccgcagt tcgggcagca cggcgccgca
    34801 ggcaacagcc gcttttcgtc acggggtagt gattcaggga acggcttacg ggtgcgggtc
    34861 tgacgcaacg gacgctgtac tgccgggtca tacaccctac cagtcagcgt atcgctctct
    34921 atcgctctct ttctgaagcc ggttcagatc ggcttccatt tgtgcgatac ggcgggagac
    34981 tttttcggaa cgactgccga agttcatccg gcggagttta tccagctgcg cctgcagatg
    35041 gtctatttcg cgctcccggt tgctcagctt ttcctgcagg gcgtggatca gcgcttcctg
    35101 ttcggccagg cgctgtttca gcaggaagat gtcgtcagaa gagatgtcgt tcataagccc
    35161 gtattttacc gggcttattc tgtgacaacc aggataaaga gatttacagc atggtcaggg
    35221 aggtcagcag ccgcttaggc tgtcgccagt cgataccttc cagcagcatc gccagctgcg
    35281 cctgtgtaag gaacactttg ccatcacggg ctgacggcca ggcgaagcgc ccacgctcca
    35341 gccgtttggt caggaggcac agtccgtcac cggtggacca cagcagttta acctgactgc
    35401 cgctgcggcc ccggaaaatg aaaacatggc cggacatggg atcgtctttc agcgccgtct
    35461 gtactttcgc agccaggccg ttgaagccat ttctcatatc ggtgataccg gcaaccagcc
    35521 aaattttggt cccggaaggt aacgggatca tcgcttcagt tcctgtatca gcagagtcag
    35581 gagcttttcg ctgacattgc cattgaagcg gagcgtcccg tgccggaacg ttacctcaca
    35641 gctgatactg agggtttccg gatcctctgc gagcgattct ggctgttcgg cagctgcatc
    35701 gagagtcaca ggaagtagct gggggctctc tgaagaaggt aatagcagct ttccctcgcg
    35761 ccattgttgt cgccatttga acaacagatt ggcgttaatg ccattttcaa gagcaagttt
    35821 tgagatggat atcccgggtt cacaggaggc agcaacgtgc tgctgtttaa attcgggagg
    35881 ataattaggg cagccttttc gcctgccggg attcacattt ttctgcatat ctgacacttt
    35941 ggttcccact acttatttgg tggacaccac tttgtctaat tcgtcagatt ctgaccagac
    36001 ggttcaggct gtacgcttac cactatttta tccctgttga atttggttaa gccggtgcag
    36061 acgcgccggg aaacgcacat ctgaaccggc acaataaaat acaatacaac cccctgttac
    36121 tggagatacg aatggaatga cgaggcagcc tgtcagtcac agggggctgt tggcataaaa
    36181 tcaacggaaa ggccaccaac accggaatta agctccacag tccccgcact ggcaatccat
    36241 tcccgggaaa ggcatcactg caaactgctc cggaggcaac cattacagcc tctgtaacgg
    36301 attatcagcc ctggcatctt ccgacggcac ctcctgccgc ccccacgc
//
LOCUS       DADTFG010000029        36112 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.029, whole genome shotgun sequence.
ACCESSION   DADTFG010000029 DADTFG010000000
VERSION     DADTFG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.029"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(208..348)
                     /locus_tag="J1138_001375"
     CDS             complement(208..348)
                     /locus_tag="J1138_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453193.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            444..1160
                     /gene="arcA"
                     /locus_tag="J1138_001376"
     CDS             444..1160
                     /gene="arcA"
                     /locus_tag="J1138_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBA8453194.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1220..2572)
                     /gene="creD"
                     /locus_tag="J1138_001377"
     CDS             complement(1220..2572)
                     /gene="creD"
                     /locus_tag="J1138_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBA8453195.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLRELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNVALNLSGTGDLSVVPGGRYS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDELRIWK
                     "
     gene            complement(2630..4054)
                     /gene="creC"
                     /locus_tag="J1138_001378"
     CDS             complement(2630..4054)
                     /gene="creC"
                     /locus_tag="J1138_001378"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBA8453196.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEINVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGMLASLRLHRHFT"
     gene            complement(4054..4743)
                     /gene="creB"
                     /locus_tag="J1138_001379"
     CDS             complement(4054..4743)
                     /gene="creB"
                     /locus_tag="J1138_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBA8453197.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4756..5229)
                     /gene="creA"
                     /locus_tag="J1138_001380"
     CDS             complement(4756..5229)
                     /gene="creA"
                     /locus_tag="J1138_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBA8453198.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5440..6309
                     /gene="robA"
                     /locus_tag="J1138_001381"
     CDS             5440..6309
                     /gene="robA"
                     /locus_tag="J1138_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBA8453199.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6306..6953)
                     /gene="gpmB"
                     /locus_tag="J1138_001382"
     CDS             complement(6306..6953)
                     /gene="gpmB"
                     /locus_tag="J1138_001382"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBA8453200.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            7005..7532
                     /gene="yjjX"
                     /locus_tag="J1138_001383"
     CDS             7005..7532
                     /gene="yjjX"
                     /locus_tag="J1138_001383"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HBA8453201.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(7605..7931)
                     /gene="trpR"
                     /locus_tag="J1138_001384"
     CDS             complement(7605..7931)
                     /gene="trpR"
                     /locus_tag="J1138_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBA8453202.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(8021..9958)
                     /gene="sltY"
                     /locus_tag="J1138_001385"
     CDS             complement(8021..9958)
                     /gene="sltY"
                     /locus_tag="J1138_001385"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBA8453203.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10165..11832
                     /gene="ettA"
                     /locus_tag="J1138_001386"
     CDS             10165..11832
                     /gene="ettA"
                     /locus_tag="J1138_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBA8453204.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11888..12172)
                     /locus_tag="J1138_001387"
     CDS             complement(11888..12172)
                     /locus_tag="J1138_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8453205.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(12174..12506)
                     /locus_tag="J1138_001388"
     CDS             complement(12174..12506)
                     /locus_tag="J1138_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA8453206.1"
                     /translation="MGKAIIFIETPIFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(12598..13830)
                     /gene="nadR"
                     /locus_tag="J1138_001389"
     CDS             complement(12598..13830)
                     /gene="nadR"
                     /locus_tag="J1138_001389"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBA8453207.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13851..15233)
                     /gene="radA"
                     /locus_tag="J1138_001390"
     CDS             complement(13851..15233)
                     /gene="radA"
                     /locus_tag="J1138_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBA8453208.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(15282..16250)
                     /gene="serB"
                     /locus_tag="J1138_001391"
     CDS             complement(15282..16250)
                     /gene="serB"
                     /locus_tag="J1138_001391"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBA8453209.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            16356..17000
                     /locus_tag="J1138_001392"
     CDS             16356..17000
                     /locus_tag="J1138_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBA8453210.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            17028..18044
                     /gene="lplA"
                     /locus_tag="J1138_001393"
     CDS             17028..18044
                     /gene="lplA"
                     /locus_tag="J1138_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBA8453211.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISLDKTPDLPNFTETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            18076..18339
                     /locus_tag="J1138_001394"
     CDS             18076..18339
                     /locus_tag="J1138_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8453212.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(18500..19219)
                     /gene="deoD"
                     /locus_tag="J1138_001395"
     CDS             complement(18500..19219)
                     /gene="deoD"
                     /locus_tag="J1138_001395"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBA8453213.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19276..20499)
                     /gene="deoB"
                     /locus_tag="J1138_001396"
     CDS             complement(19276..20499)
                     /gene="deoB"
                     /locus_tag="J1138_001396"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBA8453214.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20551..21873)
                     /gene="deoA"
                     /locus_tag="J1138_001397"
     CDS             complement(20551..21873)
                     /gene="deoA"
                     /locus_tag="J1138_001397"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBA8453215.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21951..22730)
                     /gene="deoC"
                     /locus_tag="J1138_001398"
     CDS             complement(21951..22730)
                     /gene="deoC"
                     /locus_tag="J1138_001398"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBA8453216.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22988..24538
                     /locus_tag="J1138_001399"
     CDS             22988..24538
                     /locus_tag="J1138_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBA8453217.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24510..25373
                     /gene="yjjW"
                     /locus_tag="J1138_001400"
     CDS             24510..25373
                     /gene="yjjW"
                     /locus_tag="J1138_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBA8453218.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAACEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERG
                     VSRLIFPALYL"
     gene            complement(25590..26369)
                     /locus_tag="J1138_001401"
     CDS             complement(25590..26369)
                     /locus_tag="J1138_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA8453219.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(26366..27439)
                     /locus_tag="J1138_001402"
     CDS             complement(26366..27439)
                     /locus_tag="J1138_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBA8453220.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27561..27722)
                     /locus_tag="J1138_001403"
     CDS             complement(27561..27722)
                     /locus_tag="J1138_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBA8453221.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27849..28454)
                     /gene="osmY"
                     /locus_tag="J1138_001404"
     CDS             complement(27849..28454)
                     /gene="osmY"
                     /locus_tag="J1138_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBA8453222.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28847..30436)
                     /gene="prfC"
                     /locus_tag="J1138_001405"
     CDS             complement(28847..30436)
                     /gene="prfC"
                     /locus_tag="J1138_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBA8453223.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVSIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30527..31204)
                     /gene="yjjG"
                     /locus_tag="J1138_001406"
     CDS             complement(30527..31204)
                     /gene="yjjG"
                     /locus_tag="J1138_001406"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBA8453224.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(31219..31665)
                     /gene="rimI"
                     /locus_tag="J1138_001407"
     CDS             complement(31219..31665)
                     /gene="rimI"
                     /locus_tag="J1138_001407"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBA8453225.1"
                     /translation="MNTISSLETTDLLAAFHIEQRAHTFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31634..32047)
                     /gene="holD"
                     /locus_tag="J1138_001408"
     CDS             complement(31634..32047)
                     /gene="holD"
                     /locus_tag="J1138_001408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBA8453226.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VTSPALTELRANPTARAALWQQICTYEYDFFPRND"
     gene            32150..33181
                     /gene="rsmC"
                     /locus_tag="J1138_001409"
     CDS             32150..33181
                     /gene="rsmC"
                     /locus_tag="J1138_001409"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBA8453227.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTATSRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLATNGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            33372..33458
                     /locus_tag="J1138_001410"
     tRNA            33372..33458
                     /locus_tag="J1138_001410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33406..33408,aa:Leu,seq:cag)
     gene            complement(33497..33733)
                     /locus_tag="J1138_001411"
     CDS             complement(33497..33733)
                     /locus_tag="J1138_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBA8453228.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            33874..34662
                     /gene="fhuF"
                     /locus_tag="J1138_001412"
     CDS             33874..34662
                     /gene="fhuF"
                     /locus_tag="J1138_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBA8453229.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVLQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(34700..35377)
                     /gene="bglJ"
                     /locus_tag="J1138_001413"
     CDS             complement(34700..35377)
                     /gene="bglJ"
                     /locus_tag="J1138_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBA8453230.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYASQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(35335..36060)
                     /locus_tag="J1138_001414"
     CDS             complement(35335..36060)
                     /locus_tag="J1138_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA8453231.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHSERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8607 a         9817 c         9069 g         8619 t
ORIGIN      
        1 aggataattt tataaaaaat aaatctcgat aattggattc accacgttta ttagttgtat
       61 gaagcaacta gttggattat taaaataatg tgacgaaagc tagcatttag atacgatcat
      121 ttcatcaaac tgttaacgtg ctacaattga acttgatata tgtcaacgaa gcgtagtttt
      181 attgggtgtc ctgcccctct tagcctgtta tattgctgtt aaaatggtta ggatgacagc
      241 cgtttttgac actgtcgggt cctgagggaa agtacccacg accaagctaa tgatgttgtt
      301 gacgttgatg gaaagtgcat caagaacgca attacgtact ttagtcatgt tacgccgatc
      361 atgttaattt gcagcatgca tcaggcaggt cagggacttt tgtacttcct gtttcgattt
      421 agttggcaat ttaggtagca aacatgcaga ccccgcacat tcttatcgtt gaagacgagt
      481 tggtaacacg caacacgttg aaaagtattt tcgaagcgga aggctatgat gttttcgaag
      541 cgacagatgg cgcggaaatg catcagatcc tctctgaata tgacatcaac ctggtgatca
      601 tggatatcaa tctgccgggt aagaacggtc ttctgttagc gcgtgaactg cgcgagcagg
      661 cgaatgttgc gttgatgttc ctgactggcc gtgacaacga agtcgataaa attctcggcc
      721 tcgaaatcgg tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa ctgactattc
      781 gtgcacgcaa cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa gaacgtcgta
      841 gcgttgaaag ctacaagttc aatggttggg aactggacat caacagccgt tcgttgatcg
      901 gccctgatgg cgagcagtac aagctgccgc gcagcgagtt ccgcgccatg cttcacttct
      961 gtgaaaaccc aggcaaaatt cagtcccgtg ctgaactgct gaagaaaatg accggccgtg
     1021 agctgaaacc gcacgaccgt actgtagacg tgacgatccg ccgtattcgt aaacatttcg
     1081 aatctacgcc ggatacgccg gaaatcatcg ccaccattca cggtgaaggt tatcgcttct
     1141 gcggtgatct ggaagattaa tcggctttac caccgtcaat aaaaacggcg ctttttagcg
     1201 ccgtttttat ttttctacct tatttccaga tacgtaattc atcgtccatg gtaacttctt
     1261 tactggcttt cattttcggc agtgaaaacg cataccagtc gatattacgg gtcacaaaca
     1321 tcatgccggc cagcgccacc accagcacac tggttcccaa caacagcgcg ctatcggcag
     1381 agttgagcag tccccacatc acaccatcca gcaacaacag cgcgagggta aacaacatgc
     1441 tattacgcca gcctttcaat accgcctgca aataaatacc gttcattaac gccccaatca
     1501 gactggcgat tatccatgcc acggtaaaac cggtatgttc agaaagcgcc agcaagagca
     1561 aataaaacat caccaatgaa agccccacca gcaaatattg cattgggtgt aaacgttgcg
     1621 cggtgagcgt ttcaaaaaca aagaatgcca taaaagtcag cgcaatcagc agaatggcgt
     1681 acttagtcgc ccggtccgtt aattggtatt gatcggctgg cgtcgtcact gcgacgctaa
     1741 acgcggggaa gttttcccag ccggtatcat tgcctgaagc aaaacgctca ccgagattat
     1801 tagcaaacca gctactttgc cattgcgcct gaaaacctga ctcgctaact tcccgtttgg
     1861 ctggtagaaa atcacctaaa aaactgggat gcggccagtt gctggttaag gtcatttcgc
     1921 tataacgccc gccaggcacc acagaaagat cgccggtacc gcttaaattc agcgccacat
     1981 tcagcttcag gttctgcttg cgccagtccc cttcgggtaa agggatatgc acgccctgcc
     2041 cgccttgctc taacccggtg ccgggttcaa tggtcagcgc cgtaccgtta acttcaggcg
     2101 ctttcaccac accaatacca cgcgcatccc cgacgctaat cacaataaat ggcttgccta
     2161 aggtgatatt tggcgcgtcg agttcgcgaa gacgcgaaac atcgaaatcg gcttttagcg
     2221 ttaaatcact gtgccagacc tgaccggtat agatgcctat cttgcgttct tccacgttct
     2281 gattgccatc aaccatcaat gactcaggta accaaaaatg aataaaactt cttttccgct
     2341 ccacggtttt atcgtcttcc tgcaccgtat aaagctcggt cacaggaata gcgatgagcg
     2401 gcccaacgag tttttgcggc ccgctggtac tttggcgaat cacatcttcc acatcgctac
     2461 ggtaatcggc acgttcgaca atcacctgcc gaatcagcat tatcggaatc aacaacagca
     2521 atactgcacc aaacaaggtg gtcattttcc agaacagggg ggatttcaac atagtcttct
     2581 cctttgcaat gacagcagga tacgaaggct atgtgggaag aatttgaagc tatgtgaagt
     2641 gacggtgaag tcgaagcgag gccagcatgc caccttcctg cacgttgcgc agcgtgactt
     2701 cgccgttaaa caaacgggcg acctcactga caaacgccaa ccccagaccg ctgctttttt
     2761 gcccatttgc acgcggcaaa gagtaaaagc gttcaaaaat acgtgacagc gcgtagtcag
     2821 gaatcccact accggtatcc agcaccttaa gggtgacgta ttcctgatcc acttcggcgc
     2881 ttagcgttat gcaaccgctc tcgggagtaa aatcgatggc gttatccagt aaattcccca
     2941 gcgcctgctc cagtaacgcc ggttcagcag caacgttaat ctcagtaggc ataacatgca
     3001 aagtgatgtt tttttctgcc aactgcacgg tgcgcgcttc gctgacgcgg cgaaataatg
     3061 ccgccacatc aacagcagtc agaacgactt cctgacgatt ctccagtctt gcctggcgta
     3121 gtaacgtttc caccagtgcc tgcattcgcg cattttgcgt cagaatgttg tcggtaaaac
     3181 gagccaccac ttccggcggc ggaccttcgc gtaaaatttc cgccgcgcca cgaatcgccg
     3241 ccagtgggct ttttagctca tgagttaacg cataaacata ctgctcaata tagtttttcc
     3301 cttccagctt cacgcgcata ctttccagcg cctgcgcgag tttacgcaac tcgctactac
     3361 cgagatcggg gagaggaacg ggcttattgt cagtgacgga atcagcatag cgagtgagcc
     3421 gggcaataga gcggttgatc caccaaacca tgcctgcgcc aatcaccagt gcaatcccca
     3481 acaaaatggc gctggcccat aaaattcgcc gctcgctacg cttaatgacc ggagccatcg
     3541 ccgcgttcgg tttgcctacg ctcaaaacgc caataagccg cgagccgtcc ataatcggcg
     3601 cggcaacata catcacagaa ctttcgggat cggcaggatt ttgcaacgtg ctgcgcgcac
     3661 catactgacc acgcaacgtt agccagacgt cattccagcg cgaataatcc tgtccaacgg
     3721 ctttatttgc tgaatcaaac aatactttgc cctgcgcatc ggtcatatag acatgatatt
     3781 cgttgcgcac tttgttaatg ccaccgatat tggcgcgaaa cgggcgatgt tgtagctgat
     3841 taaacgcctg cgccagttgc ccatgcgttg ggtccccaga gagcaaatcg ggacgcgcca
     3901 gctccgccag caacgttgcg gtgtcgatta acgtcccctc ggttgctctt cgcacgcccg
     3961 gtttaacttc tttgacaaaa atagccagaa cgaaccaggc cgccaccgcc accagtaaaa
     4021 aatagcccag caacaaccgc atgccgatac gcattacagg cccctcaggc tatatcccat
     4081 gccgcgatga gtattaatcg gtgaaagatc ggggttgatg gcgcgcagct tggcacgcag
     4141 cgttttaatg tgggtatcga cggtgcgatc gtaggtgtcc tgcgcatctt cccacacgct
     4201 atccatcagt tgctggcggg accagacgcg gcccggcgac ttgagtaacg tcttcagcaa
     4261 taaaaactca taccgcgtca gcgttaatgg cgtgtcaaac cagctgatct gcgccgcagg
     4321 ttcattcaat tcaaaatgac caatacggat aacgggagac ggcgtcgaga acttcttcac
     4381 ccgacgcagt aaggtgcgca ctctggcgca cacttcgcga ggtgaaaacg gtttagccac
     4441 gtagtcgtca gcgccaattt ccagcccaag caggcgatcg acctcttcac ttcgggccgt
     4501 caggaacagt acaggtaacg ccggatggag cgccagtaac tggcggcaca attcaaagcc
     4561 gctgatatca ggcaaaccaa catcgagaat catgacatcg ggaacctgct ggcgagcttt
     4621 atccagcact ggcaagcctc gctcaaagac ctcgacgtca aaaccttcct gttgcaacat
     4681 gtagaccagc gtatcggcta tcccttgctc gtcttcgact aaccagaccg tttcccgttg
     4741 cataaatcgc ctctgttatt gccgccacgg catgacagga accgcgctaa tcgcgttttt
     4801 cggcgaacct tctacaactt tgtcggagta agccagataa gcgagcgcgt tgcgtttagc
     4861 gtcataaaag cgcacgacct gtaacgactt aaagaccagg gacgtgcgtt ttttgaatac
     4921 tacctcgccc tgagctttgc cgtttttaat acgatccgac agttcaatcg gcccgacttg
     4981 ttgacaagaa atggccgcat cggaggtatc ttctgccagg cccaatcccc ctttaatacc
     5041 accggttttc gcccggctca cataacaggt gacatttttc acatcgggat cgtcaaaggc
     5101 ttcaacaaca attttgtgat ccgggccgat cattttaaat acggtgtcga ccgaaccaat
     5161 ctcttctgca tgagccaatg gccccagcat tatcaggctt aaagacagga ttaaatgctt
     5221 gtatttcata tcgttaccat ttttatctat tacacgagca attagttcgt cacggtaaat
     5281 aacatattga ttaagaacaa aatctcaata cttttatttc cgtcaagacc taaaacatac
     5341 tcgactaaga aaaaaacact gaatgctaaa acagcaaaaa atgctattat ccaattacct
     5401 gatgtcaggt gctcgttgtt gaaaggatga ggatatttta tggatcaggc cggcattatt
     5461 cgcgaccttt taatctggct ggaaggtcat ctggatcatc ccctgtcgct cgacaatgta
     5521 gcggcgaaag caggttattc caagtggcac ttacagagaa tgtttaaaga tgtcactggc
     5581 catgctattg gcgcgtatat tcgtgctcgt cgtttgtcga aatcggcggt cgcactacgc
     5641 ctgactgcgc gtccgattct ggacatcgcg ctgcaatacc gcttcgactc tcaacagaca
     5701 tttacccgcg ctttcaagaa gcagtttgcc cagactcctg cactttaccg ccgttctcct
     5761 gaatggagcg cctttggtat tcgcccgccg ctacgtctgg gtgaattcac tatgccagag
     5821 cacaaatttg tcactctgga agatacgccg ctgattggtg ttacccagag ctactcctgt
     5881 tcgctggagc aaatctctga tttccgccat gaaatgcgct accagttctg gcacgatttt
     5941 ctcggcaacg cgccgaccat tccgccggtg ctctacggcc tgaatgaaac gcgtccgagt
     6001 caggataaag acgacgagca agaggtgttc tataccaccg cattagcaca ggatcaggca
     6061 gatggctatg tattgaccgg gcatccggtg atgctgcaag gcggcgaata tgtgatgttt
     6121 acctatgaag gtctgggaac cggcgtgcag gagtttatcc tgacggtata cggaacgtgc
     6181 atgccaatgc ttaacctgac gcgccgtaaa ggtcaggata ttgagcgata ctacccggca
     6241 gaagatgcca aagcgggaga tcgcccaatt aatctgcgct gtgaactgct gattccgatc
     6301 cgtcgttaac gctgcagctc atctaatgca ggggcatcca gatgcgagat gtcccctgcg
     6361 gtttcaacca cccagccaga cgccagccac aagctttcct gataatccac gcgcgaaata
     6421 gaacagttac gcagacgtaa gcggcgctct gcccatgctg gtaatccgag aatcgtactc
     6481 accaggcatc ccagtgcaat accatggctc accagtagcg gtcggcttcc ctgcggtaag
     6541 tcccggcaag attccagtgc cgcattaacg cgatcgctga gctcctgcat tgactcgcct
     6601 tcaggaatac gcccgtcaac ggtgccattg accagttgcc gacgccagtt ctcttcttct
     6661 tcggtgagtg aatcgatatt tcttgtttcc agtacaccca tatttaattc acgcaggcga
     6721 gaatcaaaga tgatgtcaca gccgcaggcc tgggcgatga tttccgccgt gcgccgggtc
     6781 cgtcctaaat cgctactgat gatatgcgta atgccaagct ctttggcacg ggttgccacc
     6841 tgcatcgcct gttgctcacc tttggcggtc agcgggctgt cagactggcc ctgaatacgt
     6901 cgctcggcgt tccactgcgt ttcaccgtgg cggactaggt atacctgtaa catgcttttt
     6961 ttccgtatac tgctgtaacg ttaattttct gagtaatgct gattatgcac caagttgtct
     7021 gtgcgaccac caatcccgct aaaattcagg ccattctgca ggcatttcac gagatcttcg
     7081 gcgaaggatc ctgccatatt gcatccgttg ccgtcgagag cggtgtaccg gaacagccct
     7141 ttggcagtga ggaaacgcgc gctggcgcac gaaatagggt agccaatgcc cgccgtttac
     7201 ttcctgaggc tgatttttgg gtggcgattg aagctggcat cgatggtgac agcactttca
     7261 gttgggtggt gatagaaaac gccagccagc gcggcgaggc gcgttcggct actttgccgt
     7321 tgcctgcggt gattctggaa aaagtgcgtg aaggtgaggc gcttggtccc gtgatgtcgc
     7381 gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg tttaccgccg
     7441 ggaaactcac tcgcaccagc gtctatcacc aggcggtgat cctggcgctg agtccgtttc
     7501 ataatgccgt gtatcagcca ttgcaggcct gattaggcgt tcacgccgca tccggcattc
     7561 ggtgcacgat gcctgatgcg ccatgtctta tcaggcctac aaaatcaatc gcttttcagc
     7621 aacacgtctt ccagccactg gcgcagctcg acgggcgcgg ctttcagact attagatcca
     7681 cgcgtaatcg tcgcgatgcc cgcgccaagt tcatttttta actcacgctg gctcatttcg
     7741 ccgcgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc
     7801 gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg
     7861 tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat
     7921 tgttgggcca tagtatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt
     7981 actcgctaaa gagtacgata gcatatcata aacgtgtgga tcagtaacga cgtccccatt
     8041 ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag
     8101 ccagcacgtt tttcacataa ccgcgcgtct cggagaatgg aatactctcg acaaatgcca
     8161 ctgcgtcgat acgccctgcg ctgttgccaa gccaggtccg cacccgccct ggtccggcgt
     8221 tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt
     8281 aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg ctgctataac
     8341 cgggaataga gaacatcttc accgtatggg tcgcggtacc aggcataatc tgcatcaggc
     8401 cgctggcccc taccggtgat ttcacttttg gattccaggc actctcctga cgggcaatcg
     8461 ccatcgcata gctttgcggg atctccttac cactggtgta gcgtttgaaa agatcgttgt
     8521 aagccagcgg gaatcgctct tccagatgat cccacagctt cccggcgatc gttgcctgaa
     8581 cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt
     8641 ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt
     8701 acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct
     8761 gcggcgcttt atcaatcttc agctcatact cttcgccgat gcgttgtgca gcaaccatcg
     8821 ggtagaaacc acgctgttgc atgagttgat gcaaaatctc ttttgcttca gcttcacgtc
     8881 cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct ttcgcttcca
     8941 taggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca agcgccattc
     9001 gcacacggcg ttcaataagc gaagtagatt gcgagcgcat aatcgcgtca tcgcgccatt
     9061 tcgcctgctc gtcggtgaca tcgttgccca tcaaacgcca ggcgacaata tcgcgcagct
     9121 cctgaatctg atcttcatta agctgctgtg cctgagcaag cgacgggatc atcagccgtg
     9181 cattttcagc atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa
     9241 aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg
     9301 aaatgattgc cgaagcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta
     9361 ccaggccggt gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat
     9421 cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct
     9481 tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc
     9541 atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg
     9601 ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg
     9661 tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt
     9721 gattcatcag gtcatcagtg atctggcggt attccaggta gggataaagt gggtaatctt
     9781 tcagtccagg catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttaa
     9841 tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg
     9901 tcagcagaca gacaccgaca gccaacagcc gccaggtaac gtgtttggct ttttccacaa
     9961 gcgcatcctc taagtgtcaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct
    10021 aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac ggggtgatcg
    10081 actggctggg attagacagg gaaacaggct acacttcgcc tttgaaaact catcatcacg
    10141 ataaacatag aggcgaagtc caacgtggct caattcgttt ataccatgca tcgtgtcggc
    10201 aaagttgttc cgccgaaacg tcatattttg aaaaacatct ctctgagttt cttccctggg
    10261 gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt ccaccctgct gcgcattatg
    10321 gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc agccagacat caagattggt
    10381 tatctgccgc aggaaccgca gctgaacccg gaacacaccg ttcgtgagtc cattgaagaa
    10441 gcggtttctg aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc gctgtatgcc
    10501 gatccggatg ccgattttga caagctggcc gctgaacaag gccgtctgga agagatcatt
    10561 caggctcacg acggtcacaa cctgaacgtt cagctggagc gtgcggcgga tgcgctgcgt
    10621 ctgccggact gggacgcgaa aatcgctaac ctttccggtg gtgagcgtcg tcgcgtggcg
    10681 ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc tcgacgaacc gaccaaccac
    10741 ctggatgcgg aatccgtagc gtggctggaa cgcttcctgc acgacttcga aggcaccgtg
    10801 gtggcgatta cccacgaccg ttacttcctc gataacgttg cgggctggat cctcgaactt
    10861 gaccgcggtg aaggtattcc gtgggaaggt aactactcct cctggctgga gcagaaagat
    10921 cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag
    10981 ctggagtggg tacgtcaggg aactaaaggc cgtcagtcaa aaggtaaagc acgtctggcg
    11041 cgctttgaag aactcaacag caccgaatat cagaaacgta acgaaaccaa cgaactgttt
    11101 attccacctg gaccgcgtct gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc
    11161 tatggtgatc gtctgctgat tgactccctg agcttctcga tcccgaaagg agcgatcgtc
    11221 gggatcatcg gtccgaacgg tgcgggtaaa tcgacgctgt tccgtatgat ctctggtcag
    11281 gaacagccgg acagcggcac catcactttg ggtgaaacgg tgaaactggc gtcggttgat
    11341 cagttccgtg actcaatgga taacagcaaa accgtttggg aagaagtttc cggcgggctg
    11401 gatatcatga agatcggcaa caccgagatg ccaagccgcg cctacgttgg ccgctttaac
    11461 tttaaagggg ttgatcaggg taaacgcgtt ggcgaactct ccggcggtga gcgcggtcgt
    11521 ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc
    11581 aacgacctgg atatcgaaac cctgcgcgca ctggaaaacg ccctgctgga gttcccgggc
    11641 tgcgcgatgg ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac ccacattctg
    11701 gattaccagg atgaaggtaa agttgagttc ttcgaaggta actttaccga gtacgaagag
    11761 tacaagaaac gcacgctggg cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt
    11821 attgcgaagt aatgcgtaaa atgccggatg cggcgcacca gcatcgcatc cggtattagt
    11881 taaatgatta atgggaagca agttcccgga aaaaggcagg attttcttta attgtggcaa
    11941 gtacttttgc agccaatccc gtaggatttc ttcgcttcga ttcccagctt ttaatggtat
    12001 ccacactggt tcccagagct tttgccatct cactttgaga aacgttgagt tgctcacgaa
    12061 tagctttaac atccgcaatt tcgtagcggg tcacccgtgc aggttctttt aaaccttgtt
    12121 taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaaactc attttatatc
    12181 tcgcctttga gcagtttcgt caacttcttt agttgagctt tttccgtatc tgtcagacta
    12241 tctttggcat ttttcggata ggccataatc aaataaataa tctcttccgt cgccagaaag
    12301 taaattattc tgacactgcc actttttccc tgtgaacccg cagccattcg gactttacgc
    12361 aatccaccag tctgctgaat gagatcacct ttatcgggag ttccgatgag ttctttttgt
    12421 aattccttta attcatcatc ggttgcaatc tgcttgatct gacgcgtaaa tattggcgtc
    12481 tctataaata ttatcgcctt acccattacc cctccctggg gggtacattg tacaccgaaa
    12541 tggagtacta agtacacttt taaatcagta atcccactca acatcttata aagatgatta
    12601 tctttgctcc cccatcatct cccgcaccag ctccacgcag cgcaggaaac ggctgtcgta
    12661 atcgtcctct tcaacccgca cgaattcgat attgttctct tccagcatct ccaccagcaa
    12721 gttctggaac tctttgcgat ccaccgaact gccgaggctg cgtaaaccat ccgccaccca
    12781 cggcgtgttg ttctccagca ggatcaccag atcgaaacgg tattcatcaa tcaacgcctg
    12841 cacgaacgga tgctcacgcc cttcgtactt tttgcagaac gcctgggtag tgacaaaatc
    12901 ggtgtcgata aacgccactt tattggcata tttcactgca aaatcaatat attgcgcatg
    12961 tcccagagcg attttatcgt agtcggaata ctgcaacgcg atctcatcgc caccgaggtg
    13021 cgaaaagaca taatcacgac catattccca cgcactggta gtgttgaaga tattggcaag
    13081 tttgtttacc agggtggatt taccgctcga ttcaccaccg aggatcgcca cggtacgcac
    13141 aaagaatggc ttcacttccg taggaatata ttcccagtag cggaacgggt tttcgcggat
    13201 ctgcgcaccg ctgatactca taaaggtacg cttcggatcg accagcaccg tctcgatccc
    13261 cagatgttcc atatactgcg gcgcatcggc ttcttccgag gtgtagatca ggtccggctg
    13321 aatgcctttt tcggccataa actttttgat gccgttgctc cacacatccc agccgtgtgg
    13381 atacggttcc atgccctctt cgttaaaagc atgaatgcgg atattttttt gatacttaaa
    13441 ggtctgcaat aaccagcgca gacgatccgg cacggttggc tgctgcgaca tggcgctgtc
    13501 ttcgaacaat gcgcggtcgc gggtatcgtc aaaacccata atgatatgca gctcgtcaac
    13561 ctggctacag gcgcgctgga taaggtagat atgtccagta tgcagcgggt aaaacttacc
    13621 gaatacgaca ccgatcgttt tcttctgccg gggaaattca agtcccaaat aacggtgcaa
    13681 tgcctccagc ttttgtgcgc tggggctttt aattttggca ttcagtaact ggcttaaata
    13741 ccctttggtc ataccgctgg catcagctac ctgctgtaga gtgcagccct gttgcttgat
    13801 ggcagttttc aggtaatcaa atgacgacat atctccctcc gtatctctca ttataagtcg
    13861 tcgaacacgc taagcgcgtc ggagagtttt ttaacgccaa aaatctgcat cccttccggc
    13921 gcttttttcg gcacgttagc tgccggaaca attgcccggc gaaaaccgtg tttcgccgct
    13981 tctgagattc gttcctggcc gctgggcacc gggcggatct cccctgccag cccgacttca
    14041 ccaaacacca ccagatcctg cggtagcggt ctgtcacgca ggctggaaac catcgccagc
    14101 agtaacgcta aatcggcact ggtttccgtc accttcacgc caccgaccac gttaacaaac
    14161 acatcctgat cggccatttg tagaccaccg tgacggtgaa gcaccgccag caggattgcc
    14221 agacggtttt gctccagccc gactgccacg cggcgtggat tcgccatcat cgagtgatcg
    14281 acgagcgcct gaatctccac cagcagcgga cgcgttcctt cccacactac catcactgag
    14341 ctaccggagg tcacttcatc gccgcgactt aagaaaattg ccgaagggtt gctgacttca
    14401 cgcagcccct gctcggtcat tgcgaagaca cccagctcat tcaccgcgcc gaagcggttt
    14461 ttatggctgc gtaaggtgcg aaaacgtgag tcggcatcac catccaaaag caccgaacag
    14521 tcgatacagt gttccagcac tttagggcca gccaacgagc catctttggt tacgtgcccg
    14581 accatgacaa tcgccacacc gcgcgttttg gcgaagcgcg tcagataagc cgccgtttca
    14641 cgcacctgtg ccacgctgcc aggcgatgat tgtacatccg ccatatgcat cacctggatg
    14701 gagtcaatta ccatcagctt cggttgctct tcttcggcaa tcaggcagat ctgctcgatg
    14761 ctggtttccg acagcatatt aagattgtca gtcggcaggc caaggcgatg agcgcgcatt
    14821 gccacctgtt gcagcgactc ttcgccggtg acatacagcg ttttcatctg ctgcgccagt
    14881 ttgcacagcg tttgcaacag cagcgtggat ttcccggcac ctgggttacc gccaatcaga
    14941 atggcacttc ccggcaccac gccgccgcct agtacgcggt cgaactcttt aaatccggtg
    15001 gaaaaacgcg gcagctcttc aaggctgatg tcggaaagtt tctggacttt tgccactccg
    15061 gcgctaccgg catagccact gagacgctcg ttacgcgcca ccgttggcga cgcagcaaga
    15121 cgcacctcgg tgatggtgtt ccaggcatga caggcgctgc actgcccctg ccagcgcgga
    15181 taatcggccc cgcattcatt acaaacaaag gcgcgttttg gagcttttgc cacggtgtac
    15241 ctcgttaatg ctgtgccgcc cgcaggatga cgggcaggca attacttctg attcaggctg
    15301 ccagagagga tgcagaatac acccatcagg tcagcgtgac ggatggtgac ttccgttttt
    15361 tcattcactt ttggcttggc atggtaggca atccccagcc ctgccgcttt gatcatcggc
    15421 aggtcattgg ctccatcgcc aatcgccacg gtctgcgcca gcgggatttc atactcctgc
    15481 gcgaggcgag tcagagtttt cgctttgtac tgcgcgtcaa cgatgtcgcc gatcacattg
    15541 ccggtaaatt taccgtccat gatctccagt tcattggcta ccacggctgt caggcgcagc
    15601 ttgtcgcgca ggtattcagc aaagaaagta aagccgccgg aggcaatcgc cactttccag
    15661 cccagcgttt ccagcttgag taccagctgc gttaagcctg gcatcagcgg cagattttca
    15721 cgcacctgtt gcagaatgtt cgcgtcagcg cctttcagcg tcgccacgcg gctgcgcagg
    15781 ctggcggtaa aatcgagttc gccgcgcatc gcccgttcgg ttacctccgc caccctctcg
    15841 cccgttccgg ccagtttggc aatttcatca atacattcaa tctggatggc ggtggaatcc
    15901 atgtccatca ccagcaaacc cggcgtgcgc aggtgcggga ttttccccag cggcgcgaca
    15961 tccagctgcg cttcgtgggc caggcgcgta gcccgcgcgg tgagtgaacc tgccagacga
    16021 atcacctgat aatcttccac gcaccaggcg gcaacaatca ccatcgccgc gcccagtttg
    16081 ctctggtatt gggtcagacg ttgtttatcc agtccacgac catacagcag ccagccgcta
    16141 cgacctgcgt ggtaatccag tggcatcact tcatcaccac ttaatgaaag aggcagaccc
    16201 ggccataaag agacatcttc aggcaggtcg caccaggtaa tgttaggcat taaggctcct
    16261 gtaaaatcgt tcgaagcagg gaaaataacg catgaggcta ccttgtatcc attgcttctg
    16321 gcaacattaa gtctcaaatt ttcaaagggt ggaagatggc tcgcacaaaa ctgaaattcc
    16381 ggctgcatcg ggcagtgatt gtcctgttct gtcttgcctt gttagtggcg ctgatgcagg
    16441 gagcgtcatg gtttagtcaa aaccaccagc gacagcgtaa tccacagctg gaagaactgg
    16501 cccgcaccct ggcgcggcag gtgacgctaa acgttgcacc gctgatgcgt accgactcac
    16561 cggatgaaaa acgcattcag gcgatcctcg atcagttaac ggatgaaagc cgcattctcg
    16621 acgcaggcgt atatgatgag caaggcgatc ttatcgcccg ttctggcgaa agcgtcgacg
    16681 tgcgcgaccg actggcgctc gacggcaaaa aagcgggcgg ctattttaac cagcagattg
    16741 tcgaacccat tgcgggtaaa aacggaccgc tcggctatct gcgcctgacg ctcgacaccc
    16801 atacgctcgc caccgaagcc caacaggtgg ataacaccac taacatttta cgcctgatgt
    16861 tgctgctctc gctggcaatc ggcgtagtgc tgacccgcac gctgctacag ggtaaacgca
    16921 cccgctggca gcaatcgccc ttcctgttaa ccgccagtaa accggtgccg gaagaggaag
    16981 aaagcgagaa aaaagagtga cccattacta caagaaagga aatcgttatg tccacattac
    17041 gcctgctcat ctctgactct tacgacccgt ggtttaacct ggcggtagag gagtgtattt
    17101 ttcgccagat gccggctacg cagcgcgttc tgtttctctg gcgcaatgcc gacacggtag
    17161 tgattggtcg cgcgcagaac ccgtggaaag agtgtaatac ccggcggatg gaagaagata
    17221 acgtccgcct ggcgcggcgc agtagcggtg gcggcgcagt gttccacgat ctcggcaata
    17281 cctgttttac ctttatggct ggcaagccgg agtacgataa aactatctcc acgtcgattg
    17341 tgctcaatgc gctgaacgcg ctcggcgtca gcgccgaagc ttccgggcgt aacgatctgg
    17401 tggtgaaaac cgccgaaggc gatcgcaaag tctcaggctc ggcctatcgc gaaaccaaag
    17461 atcgtggctt ccaccacggc actttgctgc tcaattccga ccttagccgc ctggcaaact
    17521 atctcaatcc ggataaaaag aaactggcgg cgaaaggcat tacctcagtg cgttcccgcg
    17581 tgaccaatct gaccgaattg ctaccgggga tcacccatga gcaggtttgc gaggccataa
    17641 ccgaggcctt tttcgcccat tatggcgagc gcgtggaagc ggaaatcatc tccctggaca
    17701 aaacgccaga cttgccaaac ttcaccgaaa cctttgcccg ccagagtagc tgggaatgga
    17761 acttcggtca ggctccggca ttctcgcatc tgctggatga acgctttacc tggggcggcg
    17821 tggaactgca tttcgacgtt gaaaaaggcc atatcacccg cgcccaggtg tttaccgaca
    17881 gcctcaaccc cgcgccgctg gaagccctcg ccgggcgact gcaaggctgt ctgtaccgcg
    17941 cggatatgct gcaacaggag tgcgaagcgc tgctggttga cttcccggaa caggaaaaag
    18001 agctacggga gttgtcagcg tggatggcag gggcggtaag gtaactcgct gattaaaata
    18061 tccagggaag gaaatatgat cgatctggaa aaccaggaac gggagattat caatttaatg
    18121 ttcagccagg gtatatcctg gctaaccgcc gtgcgtatca ggcataaact atcacttgct
    18181 gaagtaagta aaatgttagg tatctccatt aattctctaa aacagatcga aaagacggaa
    18241 cggcttagtt caaacatcaa aagcaaaatg gcaggaattt atggttgtcc accagagttg
    18301 ctgatatgcc cttcctggat gactgctgaa cataaatgaa tagattcgtg aatagatact
    18361 tgaatggatt ttattttcta tccatatgaa aaataatagt aaatctataa atcagttgct
    18421 attgatgaat aaatactaaa aagccggagc agtctcccac tccggcttca cacgacaatc
    18481 gcttttcagc aaaacacaat tactctttat cgcccagcag aacggattcc agcgcgattt
    18541 tgatcatgtc gttgaaggtg gtctgacgct cagcggcagt ggtctgctcg tgagtgcgga
    18601 tgtggtcaga cacggtgcag atggtcaggg ctttcgcgcc aaattctgca gcgacaccgt
    18661 agataccagc cgcttccatt tccacgccga ggatgccgta tttttccatc acgtcgaaca
    18721 tttcgccgtc cggagagtag aacaggtcag cggagaacag gttaccaacg cgagcatcaa
    18781 cgcccagtgc ttttgccgcg tctaccgcgt tacgcaccat gtcgaagtca gcgatagcgg
    18841 caaagtcatg gtctttaaaa cggatgcggt taactttgga atcggtgcag gcacccatac
    18901 cgataacgac gtcgcgcagt tttacgtgcg gcagaacagc gccacaggaa cccacgcgga
    18961 taattttctt cacgccgaaa tcagtgatca gttctttggt gtagatggag caggacggga
    19021 tacccatacc gtggcccatt acggaaattt tgcggccttt gtaagtacca gtgaagccca
    19081 gcataccgcg aacgttgttc acttcacggg catcttcaag gaaagtttca gcaatatact
    19141 tcgcacgcag cgggtcgcct ggcatcaaaa ctacgtcagc aaaatcaccc atttctgcat
    19201 taatgtgtgg agtagccatc gttttatcct ttttgtgaca tattaaaggc ggagtcactg
    19261 ctccgcccaa atccatcaga acatggcttt gccatattcc atatcagaag taccaaaata
    19321 ttttgccaga gtctggccga tatccgcgaa ggtttcacga tgacccagcg aacctggttt
    19381 cactttcggg ccgtaaacca gtaccggaat gtgttcacgc gtgtggtcag taccggtcca
    19441 ggtcgggtca caaccgtggt cagcagtgag aatcagaata tcgtcatcgc gcagcaaaga
    19501 catcagctcc ggcagacggc ggtcgaacag ttccagaccc gcagcataac cggcgacgtc
    19561 gcgacggtgg ccccaggaag agtcgaagtc aacgaagttg gtgaagacga tggtgttatc
    19621 acccgcttct ttcatctctt tgatggtggc gtcaaacagc gcgtccaggc cggtcgcttt
    19681 cacttttttg gtgataccgc agttggcgta gatgtctgca attttaccga ccgaaaccac
    19741 ctggccgtgt ttttcatcaa ccagtttctg cagcacggtc ggtgctggcg gctcaacagc
    19801 caggtcgtga cggttaccag tacgctggaa gttaccagct ttgtcgccga taaacggacg
    19861 agcgataaca cgaccgatgt tgtagccgcc gttggtcagc tcttcacgag cgatttcgca
    19921 cagttcgtag agtttgtcca gaccgaaagt ttcttcatgg caggcaatct ggaacacgga
    19981 gtcagcggag gtatagaaaa tcggcttgcc ggttttcatg tgctcttcgc ccagctgatc
    20041 cagaatgacc gtaccggaag agtggcagtt accgaggtaa cccggcagat tagcgcgttc
    20101 aaccagttta tccagcagct cttgcgggaa gctgttttcg tgatcggaga agtatcccca
    20161 ctcaaacaga accgggacac cggcgatttc ccagtgacca gacggggtat ctttaccgga
    20221 tgacatttcg tgcgcccatg cgtacgcgcc gataacttca gcgttgccgt ccattcccgc
    20281 cggaatgaaa ccggtagaac cttcgtgtgc tttcgccagc cccagacggg tcagatttgg
    20341 cagattgagc gggcctttac gaccgttatc agcttcgcct ttggcacaag cttctgcgat
    20401 atgacccagg gtgtcagccc cgacgtcacc aaagcgttct gcatcttctg tagcgccgat
    20461 gccgaatgag tccagcacca taataaatgc acgtttcata tgttctccgt actcagtgct
    20521 tcaaatttaa atgatcagat cagtataccg ttattcgctg atacggcgat agacagttgg
    20581 tgtgctttcc ggtgctttat cggcaagttt aattgccgct ttcaccgctt tcgccgcttc
    20641 ctgccagctg ttttcgtctt tcgcgtggat aactgccagc ggacgctgac cgtctacctg
    20701 gtcgcccaga cgcgccatat cagtaaagcc gacgctgtaa tcgatggtgt cagatgcctg
    20761 acggcgtccg ccgcccattg caaccactgc catccccagc gcgcgggtat ccatttcact
    20821 gacaaaccct tcggtatcag catagactgc tttcgtcagc atcgctgtcg gcagatactt
    20881 cgcgtagttc tcaacgaagt cggtcgggcc tttttgtgcc gctaccatac gaccaaagac
    20941 ttctgccgct ttaccgttgt ccagcaccgc ctgtaatttc gcgcgcgctt cggcgtcatc
    21001 tttcgccagt ttgccggaga tcagcatctc cacgcacagt gccatcgtga catcaaacag
    21061 acgcgggtta cgatattcac ccgtcaggaa ctgcaccgct tcacgaactt caaccgcgtt
    21121 acctgcactg gaggccagta cctgattcat atcggtgagc agcgcggtgg tgcgcacgcc
    21181 agcgccgtta gccacgccaa caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg
    21241 cataaacgcg ccgctaccca ctttcacgtc catcaccagc gcgtccagac cttccgccag
    21301 tttcttcgcc agaatagagg cggtgatcag cgggattgaa tccacggttg cggtaatatc
    21361 gcgggtcgcg tagaaacgtt tatccgccgg agccaatgag ctggtctggc cgataatcgc
    21421 cacgccgacg tctttaataa tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc
    21481 agggatggat tccagtttgt cgagcgtacc gccagtatga ccgaggccgc gaccggagat
    21541 catcggaata tagccgccac aggcagcgac catcggcccc aacatcagcg aagtcacatc
    21601 gccgacaccg ccggtcgagt gtttatcaac aatcgggcca ttcagatgca ggcttttcca
    21661 gtcgagaacg gttcctgaat ctcgcatcgc catggtcagc gagacacgct caggcattgt
    21721 catatcgtgg aagaaaatgg tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt
    21781 gtcgcgaatg ccgttgataa agaaacgaat ttcttcatca ctcagcgcat gaccatcacg
    21841 ttttttacga ataatttctt gtgcgagaaa caaggtaacc tcccgtggaa aagagacgag
    21901 gtaatcgggg aaccggatga gcatcgctgc atcatccggc aaatcaagcc ttagtagctg
    21961 ctggcgctct taccgtcgcc gtggcccaga gctttcagca ggcttgccag caagctggaa
    22021 gcaccaaagc ggtagtgacg cgcatcagcc cagtcagcac cgaacagttc atctgcaatg
    22081 gcgagatatt tctgcgcatc ttccgcagta cgcacgccgc ccgccggttt gaaaccaacg
    22141 gttttttcta cgcccatatc gcggatcact tccatcatga tgcgcgcgct ttccggcgtc
    22201 gcattcacag ccactttacc ggtagaggtt ttgatgaagt cagcacccgc tttgatggag
    22261 atttcagacg ctttacggat cagcgcttcg tctttcagtt cgccggtttc aataatcact
    22321 ttcagcagta cgttcgccgc agcgcaagcc tctttacagg ctttcaccag gtcaaaacca
    22381 acctgctcgt tacccgccat cagcgcgcgg tacgggaaca ccacgtcaac ttcatcggca
    22441 ccgtaggcga ttgccgcacg ggtttctgcc agcgcaatgt cgatgtcgtc gttaccgtgt
    22501 gggaagttag ttaccgtagc gatacggatt tccggggtgc cctgctcttt cagagttttg
    22561 cgagcaatcg ggataaagcg aggatagata cagatagcgg cggtattgcc gaccggagtt
    22621 ttggcctgat gacacagggc gatcactttc tcatcggtgt cgtcgtcatt cagggtggtc
    22681 aggtccatca atttcagtgc acgcaggctg cttgctttca gatcagtcat ttcattctcc
    22741 tggcttgtcg ccaataaaat tcaccttgcg agtttgttag tattctaaca tctactccgc
    22801 aacacacttc gatgtacatc acaattaagg aaatctactt acaagtttgc atcactgtaa
    22861 tgcgatctgg ttcaaataat tcactttcaa atgaatgcgt cagtggtggc aaacgtatca
    22921 ggatcaaagt gaacatcacg aaacttctta caatggcgca tcaccttcgg cataaaagga
    22981 aaagaagatg cccacttctc atgaaaatgc actgcaacaa cgttgccagc aaattgtcac
    23041 cagcccggta cttagcccgg agcagaagcg ccattttctg gcactggaag cagaaaataa
    23101 tctgccttac ccacagctgc ctgccgaagc ccgccgcgcg ctggatgagg gggtaatctg
    23161 cgatatgttt gaaggtcatg cgccgtacaa accgcgctat gtcttacccg attacgcccg
    23221 ttttttggcc aacggttccg aatggctgga gctggaaggc gcaaaagatc ttgatgacgc
    23281 actctctctg ctgaccattc tttaccacca tgtaccgtcg gtcacatcga tgccggtcta
    23341 cctggggcaa ctggatgcgt tgttgcaacc gtatgttaga attctaacac aagacgagat
    23401 cgatattcga ataaaacgtt tctggcgtta cctcgacaga accctgccag acgcctttat
    23461 gcatgccaat atcggcccgt ctgattcgcc cattacccgt gcgatattac gtgcagacgc
    23521 agagctgaag caggtttcac cgaacctgac ctttatctac gatcctgata tcacccctga
    23581 tgacctgctg ctggaagtgg cgaaaaacat ctgtgaatgt agcaaaccgc acatcgccaa
    23641 cggtccggtg catgataaaa ttttcacaaa agggggctac gggattgtga gctgttacaa
    23701 ctcactgccg ctggcgggtg gtggcagcac gctggtacgc ctaaacctga aagccattgc
    23761 cgagcgcagt gaatcgctgg aggacttctt tacgcgcact ctaccgcact actgccaaca
    23821 gcagatcgcc atcatcgatg cgcggtgtga attcctctat caacaatcac acttctttga
    23881 gaatagcttc ctggtgaaag aagggctgat taaccctgaa cgttttgtgc cgatgtttgg
    23941 catgtatggg ctggcggaag cggttaactt gctgtgtgaa aaagaaggga ttgccgcacg
    24001 ctacggtaaa gaggccgccg caaatgaagt gggttatcgc attagcgcgc aactggcgga
    24061 gtttgtcgcc aatacccccg tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca
    24121 gtcggggata agttccgata tcggcaccac gccgggcgcg cgtttaccat atggcgatga
    24181 gccagatccg attacccatc tgcaaactgt cgccccgcat catgcttatt attattccgg
    24241 catcagcgac attctgacgc tcgacgaaac catcaaacgt aatccgcagg cgctggtaca
    24301 gctttgcctc ggtgccttta aagccggaat gcgtgaattt accgccaatg tcagcggtaa
    24361 cgatctggtt cgcgttaccg gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc
    24421 cgaaggttca cgcaccaaca ccacctggct gggagaagaa gccgcacgca acactcgtat
    24481 tctggaacgc cagccgcgcg tgataagcca tgaacagcag atgcgcttta gtcagtaaga
    24541 ttatcccctt ctcctgcgtt gacgggccag gcagtcgtct ggctctgttt ttgcagggct
    24601 gcaatctgcg ctgcaaaaac tgtcacaatc cgtggacgat ggggcgctgt aatgattgcg
    24661 gagagtgcgt gccgcagtgt ccgcatcagg cgttgcagat tgtcgacggc aaagtggcgt
    24721 ggagcgcggc tgcctgcgag caatgtgata cctgcctgaa gatgtgtccg caacatgcta
    24781 cgccaatggc gcaatccatg agcgtggacg aggtgcttaa ccatgtccgc aaagcggttc
    24841 tgtttattga agggataacg gtaagcggcg gcgaagccac gacccagttg ccgtttgtgg
    24901 tggcgctgtt tactgccatc aaaaacgatc cgcaactgtg ccatctcacc tgcctcgtgg
    24961 acagtaacgg catgttgagc gaaaccggct gggaaaaatt gctcccggtg tgtgacggcg
    25021 caatgctcga tctcaaagcg tgggggagcg aatgtcatca gcaattaacc ggacgcgata
    25081 atcagcagat caagcgcagc atctgtttgc tggcagagcg cggtaagctg gcggaactgc
    25141 gtttgctggt aatccctggc caggtggatt atttgcaaca tatcgaagaa ctggcggcgt
    25201 taatcaaggg acttggcgat gttccggtac gcctgaacgc gtttcatgcc cacggcgtgt
    25261 atggcgaggc gcaaagctgg ccgagcgcca cgccggagga tgtggaacaa ctggctgatg
    25321 cgttacggga gcgcggggtg agccggttaa tatttccggc gctatatttg tgacatgccg
    25381 gatgtggcct acggatcggc acagacctgt aggcatgata agacgcgtca agcgtcgcat
    25441 caggcattgt gcgccagctg ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg
    25501 cacagacttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt gcgccaacgg
    25561 ccggatgcgg cgtaaacgcc ttgtccggcc tacggcacgt taaacaacgt ataggtgtta
    25621 ttaagcaaca cttcggcaat ctcatccgcc ggttccggtc gtaactcgca aagcacggca
    25681 aacacacggg cggcctgttc agggcgatta ggctgcccct gaaaaccatt gagcggcata
    25741 tctggtgcgt cggtttccag caacaacgat gccagcggta attttgcgat gacatcgcgg
    25801 gttttactgg cgcgtggata ggtgatagtc ccgcctacgc caattttgta gcccagttgc
    25861 acaaaccgtt cggcctgttg caggctgccg gaaaaaccat gaaccacgcc agtgcacggt
    25921 aaatcgtggc gtttaagatg catcgccagt ttgtcgtgcg tgcgtcgtga atgcaggatc
    25981 accggcagat cgtagcgttt cgccagtttc agttgttcgt cgagtaacca ctgctgcctc
    26041 tcaaattgcg gatcgtcgcc aaagagatcc agaccgatct cccccaccgc caccaccttc
    26101 gccggatgcc tttccagcgc ctgctgtagc tgatcaagag acacatcgct atgtttttcc
    26161 aacataccag gatgcaagcc caatgcggca tacagcggtt gatatttttc cgctaacgcc
    26221 tgcacgcggg caaaattttc cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct
    26281 tgtgccgcgc gttgcaggct ggcctcttca tcgccactaa acggcgggaa atcaaaatgg
    26341 cagtgggtgt cgataaaacg gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt
    26401 tcgcctgcgg agcgtcgctc acttcggcaa ccagcgtatc gttggcgact ggcgctggcg
    26461 gaataacgat cgactccggc gtcaccaccg gcaccagatg gcggctcagc ggcgcttttt
    26521 cagtcaataa tttaccgacc gttgccagaa aataacgccc acataaacgc ccaagtttat
    26581 agtcttcacg cagcgccgga atccgactgc cgagcgcaat actatgcaat ggcttcggcg
    26641 gataaatttc gaatatccgc agctggcccg gtggtttctc aataaattgc tgaatctcac
    26701 gatagctggt ttcatgatgc tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca
    26761 gccagcgttc catgcgtttg aaccactgtg gcgtgtagta catttgtgac ggcacagtgc
    26821 ggatgacgac caacgttttt gccccctgcc ttgccgcctc tttaaccggg atcgcatcac
    26881 tgatcccacc atccaggtag ttaatacctt ccagcgacac tccgctacga taaaagccag
    26941 gtatcgcact ggaggcgcga atcacatcca gccagttttg tttagttggc aaaaagtaat
    27001 tcggcgcata gtcatcctga cgacaggcgc acatataaaa cgatttgccg ctgtcaaata
    27061 accgcgctgc ggtgtccatt tgcaacggca tctggcttgc ggtggcctcc accagccagt
    27121 cgagatcgat aagatttcct ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg
    27181 tggtgtaacg catgatgact ttgcgcgcgt aaccgggctg attgcagata tacgccgaga
    27241 ggttctgcgc cccggcagat gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca
    27301 taaactcatc cagcacgcca gccgtgaaaa ttccacgttg tccgccgcct tcgcacacca
    27361 gagcaatccg tccaggctgg aacggcctta gcgacaacgg cgcaatatta ccaagcgtta
    27421 caggtattcg ctgccccacc tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa
    27481 cgcaatttat tattcgctaa aaccgcaaaa gccagtccgc tggactggct ttggataaaa
    27541 ttttcttaat ggtctgaaat ctagggtcgt tttcggccca tgaacaaact caccaggaac
    27601 agaataatcc cgacgacaaa gacaatttta gctgcgcctg cagcggtacc ggccagacca
    27661 ccaaacccaa gtgcggcggc gattaacgcg ataaccagaa atatgatgcc ccaacgaaac
    27721 ataagcgtct cctttaccat agttaatgtc accgcttgat atgagcgtga aaatcactca
    27781 atgcgatatt tttagtgtgg tgcacatcgc gcctcccgac aaagttcggg aggacgaatt
    27841 acgacgaatt acttagtttt cagatcattt ttaacgcttt tcacaccatc taccgctttg
    27901 gcgatacttt cagcacggtc gctctgtgcc tgagaatcta cggtaccgga gagctggacc
    27961 acgccatcgg tggtttcaac tttcacatga cgggaaggga cgatatcgtc cgccagcagt
    28021 ttggctttga tttcactggt ggtggcggtg tcacccgcgt agcccttcac agagccttct
    28081 ttagcgtcgc gaacgtgcag tttgtcgctg acagaggtca cgccctcaac acctttcgcc
    28141 actttcaccg cctcttcggc ctgagcctgg ctttcaacga aaccgctcag ggtcacgact
    28201 ttttgatcgg ttttgacaga gatatcggtg ctcttgatgt tgtcatggtc caccagggcc
    28261 gccttcactt tcgcggtgat ggcgctgtca tccatgaaat taccgacttt attcatagag
    28321 ctatcgactt tttgccctgc gctttcattg gtagtctgcg cgttgttttc tgcgtaggca
    28381 gagccggtcg cgacggcaga ggtcaacatt acagccagca gagttttcga aatcttcagt
    28441 cttgtcatag tcatcgattt attcctgtat gtttgctcgt aatttgagcc tggcaacacg
    28501 aggttgcatt gctgaatagg gagagacttc accctctaca gaagtcaatg gtcgccatca
    28561 caaaagcgat gggtgatgaa taacgaccat tacagcctct gaatcagtta ttaatatcgg
    28621 tagaatgaca atcgacggct ttagatactg atatctacgc attgaacggt atttaacgcc
    28681 gtcagaaatg tcattacttt gttaaatata gatcacaatt ttgaaaccgc tcgggatatc
    28741 agcgaaaaca taagcaaaag tgaatgtttt aagaacattc cgtaagcggc taataaggaa
    28801 gggaaattga cagggcgcag cggctaccgc gccctggagg ctagaattaa tgctcgcggg
    28861 tctggtggaa ctgaacgtcc ggataacgtt cctgcgccag gcgcagattg accatgctgg
    28921 tagcgatgta agcgaggtta tcgccgccat caagcgccag ttggctttcg ttcttacgct
    28981 tgaactcttc gaatttcttc gcgtccgcac attctaccca gcgggcggtg gcaacgttga
    29041 ctgactcata cactgcttca acgttgtatt cgctcttcag gcgcgctacc accacatcaa
    29101 actgcagcac accaaccgcg ccaacgatca gatcgttgtt ggagatcgga cggaacacct
    29161 gcaccgcccc ctcttcggaa agctgtacca gccctttgag cagctgtttt tgcttcagcg
    29221 gatctttcag gcggatacga cggaacagtt ctggcgcgaa gttcggaata ccggtgaact
    29281 tcatcatctc accctgggta aaggtatcgc cgatctgaat ggtgccgtga ttgtgcaggc
    29341 cgaggatatc gcccggatac gcttcttcaa cgtgcgaacg gtcgcctgcc ataaaggtca
    29401 gcgcgtcgga gatcactaca tctttcgcgg tgcgcacctg gcgcagcttc atgccttttt
    29461 catatttacc ggataccacg cgcataaacg ccacgcggtc gcgatgtttc gggtccatgt
    29521 tggcctgaat tttaaatacg aagccggtaa atttatcttc gctcgcttct acggtacggg
    29581 tatcggtctg acgcggcatc ggcgcaggcg cccactccac caggccatcc agcatatgat
    29641 cgacgccgaa gttacccagc gcagtaccaa agaataccgg ggtgatttcg cccgcaagga
    29701 acagctcttt gtcgaactca ttagacgcgc ctttcaccag ctccagttcg tcacgcagct
    29761 gctgtgccag atcttcacca accgcagcgt cgagatccgg gttattcagc cctttaacaa
    29821 tgctgacttc ctgaatggtg tggcctttac cgctctgata gagataggtt tcatctttat
    29881 aaaggtggta aacgccttta aacagcttgc cgcagccaat cggccaggtg atcggtgcgc
    29941 agccgatttt cagctcgttc tcaacttcat cgagcagttc catcgggtcg cggatatcac
    30001 ggtcaagttt gttcataaag gtgaggattg gcgtatcgcg cagacgggta acttccatca
    30061 gcttacgggt acgatcttct acaccttttg cggcgtcgat aaccatcagg cagcagtcca
    30121 ccgccgtcag ggtacgatag gtatcttccg agaagtcttc gtgccccggg gtatcgagca
    30181 agttaaccag gcagtcgtga tacggaaact gcatcacaga cgtagtgatg gagatcccac
    30241 gctgcttttc catctccatc cagtccgact tagcgtgctg gttagagcca cggcctttta
    30301 ctgtaccggc ggtctgaatg gcctgtccga acagcagcac cttctcggtg atggtagtct
    30361 taccggcgtc cgggtgagaa ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat
    30421 aaggagacaa cgtcataatt ctttcttctt gagtaagcgg catcgcgccg cgcttgttga
    30481 aaacgaaaaa ttgcggctat tttacccatc aacggggggg aggcaatcag tgtttacaca
    30541 ggagctgctc cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt
    30601 gctcgcgatt gtgcgcattc agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa
    30661 tatcggactc ggcagtgtca ccaaccatca gcacgcgtga acggtcagga ttgcccgctt
    30721 gttccagcgc ataatcgaaa attttcttat tcggtttggc aacgccaact tcttcggaaa
    30781 tcaccagcaa atcgaagtaa tcacgcaggc ccgtgcgttc cagacgtacc tgttgcaagg
    30841 cgctaaagcc gttggtgatg atgccgattt tggcgttgcc acgaatggcg ttaagcaggg
    30901 aaaccgcccc cggcaacggc gtacagattt ccgccatcgc attaataaag gcctcgttga
    30961 gtttacccgg ttcgacgttc agccgttcgg cccagctctc aaaccgcccg tgctgaagct
    31021 gtaatgaagt gatcgcgccg ttttgataat ccacccacag tggcttgtta acggcctgat
    31081 agtcctgaaa atcttcagcg gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc
    31141 cggtgaatga gtcaaaggta aacagcgttt catcggcatc aaagaaaatc cagtcccact
    31201 tcattattcc accttgtctt acatactgat tggtaacgcc atgatgatgg cgtcttcgcg
    31261 accgtccgtg gtggggtagt aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc
    31321 gtacagggca atggctgcag catttgaggc acggacttcc agccatagcg tcgccacgcc
    31381 gcgtttttcc agttcgtcga tcagatgttc cagcagcgcc cttcccaatc cctgacgctg
    31441 atagtcagga tcgaccgcaa tattgaacaa tgtagcttca tccagcacca cttgcgtaat
    31501 cgcaaacgcc gccattttgc cgttttgcgt taactgaaag ttgagatagc gctcgccctg
    31561 gttgctggca aaggtttttt cactccacgg aaaggtgtgg gcgcgttgtt caatgtggaa
    31621 agccgctagt aaatcagtcg tttcgaggga agaaatcgta ttcatatgtg caaatttgtt
    31681 gccataacgc ggcgcgtgcc gttgggtttg cccgtaattc ggtgagcgcc ggtgatgtca
    31741 cctgagcgcc ttccagtgat agcggttcgt cagtacccaa ccgccagctg ttgcagcggc
    31801 tgccttgcgg cagcatcgcg attttttctg gcgtcagttg cagcacttga tcggggctga
    31861 cggttaacgc acgcagaaca tcgctcacca gaggatcggt cagggcggga agatcgtttg
    31921 ccaccatcac cagacggacg tgtggcggga tcgcaatggc aatctccccc tgcaacgcgc
    31981 cagggcgacg cagcgaccac tgggtaatgc ccagttgctg taactgccag tctcgtcggg
    32041 atgtcatagc gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg
    32101 cgccaacata tggctataat cgccgccagt atcaattgag gagcattcca tgtctgcatt
    32161 taccccggca agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct
    32221 gtttgccgga gacttacagg atgacctgcc cgcgcgttta gataccgcga ccagccgtgc
    32281 tcatacccag caattccacc actggcaggt gttaagccgc cagatggggg ataacgcccg
    32341 ttttagcctg gtcgccacgg cagatgacgt cgcagattgc gatacgctga tctactactg
    32401 gccgaagaac aaaccggaag cccagttcca gttgatgaat ttactttctc tgcttccggt
    32461 gggcacggat atttttgtcg ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat
    32521 gctggccgat tatgcaccgt tgaataaggt agacagcgcc cgtcgctgcg gcctctattt
    32581 tggtcgtctg gaaaagcagc cggtatttga tgccaataaa ttctggggcg aatacagcgt
    32641 cgatggcttg acggtcaaaa cgctgccggg cgtgtttagc cgcgacggtc tggatgtcgg
    32701 cagtcagttg ctgctctcga cgctaactcc acacacgaaa ggtaaagtgc tggatgtcgg
    32761 ctgcggcgca ggcgttcttt ccgttgcttt tgcgcgccat tcaccgaaga tccgcctcac
    32821 tctgtgtgat gtttctgcac cagcggtaga agccagtcgc gcaacacttg cgaccaacgg
    32881 tgttgaaggt gaagtctttg ccagcaacgt cttttccgag gtgaaaggtc gctttgatat
    32941 gataatctcc aacccgccgt tccacgatgg gatgcaaacc agcctggatg cggcgcaaac
    33001 gctgattcgc ggcgcggtgc gtcatcttaa cagcggcggc gagttgcgaa ttgtagcgaa
    33061 cgccttcctg ccttacccgg acgtgctgga tgagacattt ggtttccacg aagtgattgc
    33121 gcaaaccggg cgcttcaagg tgtatcgcgc cattatgacc cgccaggcga agaaaggtta
    33181 agtatctcgc cgcatgcctt gtcggatgcg gcgtacacgc cttatctgac cgacaaacga
    33241 attatccaca atccgctgat ggacattttt ccagcaatta cacctctgtc gataattaac
    33301 tattgacgaa aagctgaaaa ccactagaat gcgcctccgt ggtagcaatt ctttttaaga
    33361 attgatggta tgcgaaggtg gcggaattgg tagacgcgct agcttcaggt gttagtgtcc
    33421 ttacggacgt gggggttcaa gtcccccccc tcgcaccaat tatctttact tcttttcttg
    33481 tctcttcact gttttatcac ccctgattca aattcattat catcagcatg atgccagcaa
    33541 taagcgccag gcacgatggt agcaaaacgt aatgccgtaa ctgtttgtga tacagcatca
    33601 ccggcgtcaa taccaatccc atcagaatga caatccgcca ttgatccggc gataaatccg
    33661 catacatcag ccctgcaatc agcaatcccg gcagcaacac tccccagcca ctgcccagcg
    33721 tacgttgcaa catggtttca tctctttcat tgataatgat aaccaatatc atatgataat
    33781 ttttatcatt tgcaagccag agaaatccct tgctatcggg taaacctatc gataggatta
    33841 gcaataatta tcatttagat tactatcacg attatggcct atcgttccgc accgctctat
    33901 gaagatgtta tctggcgaac gcatctccag ccgcaggatg ctgggcttgc acaagccgtg
    33961 cgggcgacga tcgccgaaca tcgtgaacat ttgctggagt ttatccgcct ggatgaaccc
    34021 gccccactta acgccatgac gctggcgcaa tggtcatcac ctaatgcgct aagttctcta
    34081 ctcgcagtct attccgatca tatctaccgc aaccaacctt tgatgatccg cgaaaacaaa
    34141 ccgctgatct cactgtgggc gcaatggtat atcggcctga tggtaccacc attaatgctg
    34201 gcgctgctga ctcaggaaaa agcattagat gtgtcgccgg aacatgtcca cgttgagttt
    34261 cacgaaaccg gacgcgccgc ctgtttctgg gtcgatgtgt gtgaagataa aaacgcaaca
    34321 ctacattcgc cgcagcagcg aatggaaacg ttaatcagcc aggcgctggt tccggttctg
    34381 caggcactag aagcaacggg agagattaac ggcaaactta tctggagcaa taccggttat
    34441 ttgatcaact ggtatctgac tgagatgaaa cagctgctcg gcgaggcgac ggttgaatcg
    34501 ctgcgctatg ccctcttttt tgagaaaacg cttactaccg gtgaagataa tccactctgg
    34561 cgtaccgtgg tgctacgcga cggcctgctg gtgcgccgca cttgctgcca gcgttatcgc
    34621 ttaccggatg tgcagcaatg tggcgattgt acgctgaaat gaaagcactg ccggggaagt
    34681 aaacccggca tcatgcggat taataaggat gcaacacatt acttgtttcg caatgccgca
    34741 tacataacag aatatctgcg gcctccaaca accctgcatc agaactgacg cccagtttcg
    34801 acatcacatt aaatttatgt gcacggatcg ttttaatatt gcgtttaagc tgctcggcaa
    34861 tttgtgtcat tgagtagcca cgcgacataa agcgcaatat ttctctctcc gtcgggctta
    34921 acgtccggct ttggttaatg taccactgat tgttcagtcg atcggtcgcc tgacgtacac
    34981 catttaatga caaaaagagt tcctgatgaa aaatctccag cgttgacgct ttacttaata
    35041 caccgtccag cggtgatggc gacaatgaac caatcagccg agcttcaata tcatcatccg
    35101 caataactaa acgccgggta cgcggaaact taatcgccag ttcagtcagg caggataatc
    35161 cctctctgcg cgcacttctc atggcagaaa aagaaaaaat gaccgccgca aacgagatcc
    35221 gcgacatggc atcctgaaat gactcctgac tggcataggt atggagctta taagggttac
    35281 ccgcaaactt tctgaataaa ctctcaatac cgatactact catgacgcat ttttctatga
    35341 gtgcgacatt tctcttctta attcggctgt gttccattct gcggcatctc cataaacaga
    35401 aggcaagtta agttctcttg caccctgcgc gctatttatc catgcataca tttcagcgtt
    35461 gctgtgaatt gccagtcgac gcatcgcact gtttttttgg gcgctaatag ttttattact
    35521 tttcttaagc aatgaagcta tctggttgat gccccatcct ttacctaata gccgcagtac
    35581 cttgcgctct gaaagagtga gaatgacgga gcgatagcta aaatcatgag tttcagtcat
    35641 tgccggagag gtcagcatgg cgctgatacg ctctgagtgc gtattgccgg agcgtacggt
    35701 cttgaccaga ttctcaatgg gttcaacatc agacaataac gtcgccgtag ggcacatgag
    35761 caattctact gcctgggaat accaggagcg tgacaccatg aaaacccagt gaatttcccg
    35821 atattgtgag attaaagagt aataatgctc acaaacggaa cgtggatctt cactttcacc
    35881 ggccaaatca gcaatgacta atccggaacg acgtaattgt aataaggtaa ggtcctccgc
    35941 agaggcgctg gatattattt catattcagg gaagtgagtc ctcatgacct cttttaaccc
    36001 tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc ctggcaacat
    36061 atttcctcca catccactgt ctaaaaatca tgatggttta ttcaaggtat tc
//
LOCUS       DADTFG010000030        35636 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.030, whole genome shotgun sequence.
ACCESSION   DADTFG010000030 DADTFG010000000
VERSION     DADTFG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.030"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            88..372
                     /gene="pduA"
                     /locus_tag="J1138_001415"
     CDS             88..372
                     /gene="pduA"
                     /locus_tag="J1138_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HBA8453232.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            369..1181
                     /gene="pduB"
                     /locus_tag="J1138_001416"
     CDS             369..1181
                     /gene="pduB"
                     /locus_tag="J1138_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HBA8453233.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     EHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1200..2864
                     /gene="pduC"
                     /locus_tag="J1138_001417"
     CDS             1200..2864
                     /gene="pduC"
                     /locus_tag="J1138_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HBA8453234.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2874..3542
                     /locus_tag="J1138_001418"
     CDS             2874..3542
                     /locus_tag="J1138_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HBA8453235.1"
                     /translation="MEINEKLLRQIIEGVLAEMQTSDKSVSFRAPVSATVSSVPDTEN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3557..4075
                     /gene="pduE"
                     /locus_tag="J1138_001419"
     CDS             3557..4075
                     /gene="pduE"
                     /locus_tag="J1138_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HBA8453236.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPSASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAQICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4085..5917
                     /locus_tag="J1138_001420"
     CDS             4085..5917
                     /locus_tag="J1138_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HBA8453237.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSLVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTCPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTMRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDMTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5907..6257
                     /locus_tag="J1138_001421"
     CDS             5907..6257
                     /locus_tag="J1138_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase"
                     /protein_id="HBA8453238.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6277..6552
                     /gene="pduJ"
                     /locus_tag="J1138_001422"
     CDS             6277..6552
                     /gene="pduJ"
                     /locus_tag="J1138_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HBA8453239.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6576..6998
                     /locus_tag="J1138_001423"
     CDS             6576..6998
                     /locus_tag="J1138_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBA8453240.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLHANKRGE"
     gene            7001..7633
                     /locus_tag="J1138_001424"
     CDS             7001..7633
                     /locus_tag="J1138_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBA8453241.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKLLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IKGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7630..8121
                     /gene="pduM"
                     /locus_tag="J1138_001425"
     CDS             7630..8121
                     /gene="pduM"
                     /locus_tag="J1138_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HBA8453242.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQEIVHQY
                     ASLQIRYVDLPLLRQLAGNETSDRAAIQIHEALAWGLHIQLSLQCHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHRDRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8125..8400
                     /gene="pduN"
                     /locus_tag="J1138_001426"
     CDS             8125..8400
                     /gene="pduN"
                     /locus_tag="J1138_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HBA8453243.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8412..9419
                     /gene="pduO"
                     /locus_tag="J1138_001427"
     CDS             8412..9419
                     /gene="pduO"
                     /locus_tag="J1138_001427"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HBA8453244.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQLRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETIVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9416..10801
                     /gene="pduP"
                     /locus_tag="J1138_001428"
     CDS             9416..10801
                     /gene="pduP"
                     /locus_tag="J1138_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HBA8453245.1"
                     /translation="MNNSELETLIRNILREQLVPATPRMQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESASETGMGNKEDKYLKNKAALNNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKYVSLKLITTIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VASVADYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAASLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10812..11924
                     /locus_tag="J1138_001429"
     CDS             10812..11924
                     /locus_tag="J1138_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBA8453246.1"
                     /translation="MKTFSLQTRLYSGTGSLNILSRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHALAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11921..13264
                     /locus_tag="J1138_001430"
     CDS             11921..13264
                     /locus_tag="J1138_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfC"
                     /protein_id="HBA8453247.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQTEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13267..13821
                     /gene="pduT"
                     /locus_tag="J1138_001431"
     CDS             13267..13821
                     /gene="pduT"
                     /locus_tag="J1138_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HBA8453248.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13821..14171
                     /gene="pduU"
                     /locus_tag="J1138_001432"
     CDS             13821..14171
                     /gene="pduU"
                     /locus_tag="J1138_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HBA8453249.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14176..14619
                     /gene="eutP"
                     /locus_tag="J1138_001433"
     CDS             14176..14619
                     /gene="eutP"
                     /locus_tag="J1138_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBA8453250.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIYYQKTQAIVWSPATIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVKNNGVTELFALLHTEEGCR"
     gene            complement(14719..15048)
                     /locus_tag="J1138_001434"
     CDS             complement(14719..15048)
                     /locus_tag="J1138_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBA8453251.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15220..16278)
                     /locus_tag="J1138_001435"
     CDS             complement(15220..16278)
                     /locus_tag="J1138_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA8453252.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16476..16949)
                     /gene="sbmC"
                     /locus_tag="J1138_001436"
     CDS             complement(16476..16949)
                     /gene="sbmC"
                     /locus_tag="J1138_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBA8453253.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDNAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17068..18234)
                     /gene="dacD"
                     /locus_tag="J1138_001437"
     CDS             complement(17068..18234)
                     /gene="dacD"
                     /locus_tag="J1138_001437"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBA8453254.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            18443..19870
                     /gene="sbcB"
                     /locus_tag="J1138_001438"
     CDS             18443..19870
                     /gene="sbcB"
                     /locus_tag="J1138_001438"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBA8453255.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEATFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19944..21302)
                     /gene="plaP"
                     /locus_tag="J1138_001439"
     CDS             complement(19944..21302)
                     /gene="plaP"
                     /locus_tag="J1138_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBA8453256.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21292..21354)
                     /gene="yoeI"
                     /locus_tag="J1138_001440"
     CDS             complement(21292..21354)
                     /gene="yoeI"
                     /locus_tag="J1138_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBA8453257.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21569..22498)
                     /locus_tag="J1138_001441"
     CDS             complement(21569..22498)
                     /locus_tag="J1138_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8453258.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22544..23368)
                     /locus_tag="J1138_001442"
     CDS             complement(22544..23368)
                     /locus_tag="J1138_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA8453259.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23451..23705)
                     /gene="yoeB"
                     /locus_tag="J1138_001443"
     CDS             complement(23451..23705)
                     /gene="yoeB"
                     /locus_tag="J1138_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBA8453260.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23702..23953)
                     /gene="yefM"
                     /locus_tag="J1138_001444"
     CDS             complement(23702..23953)
                     /gene="yefM"
                     /locus_tag="J1138_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBA8453261.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            24236..24286
                     /gene="hisL"
                     /locus_tag="J1138_001445"
     CDS             24236..24286
                     /gene="hisL"
                     /locus_tag="J1138_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBA8453262.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            24432..25331
                     /gene="hisG"
                     /locus_tag="J1138_001446"
     CDS             24432..25331
                     /gene="hisG"
                     /locus_tag="J1138_001446"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBA8453263.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            25337..26641
                     /gene="hisD"
                     /locus_tag="J1138_001447"
     CDS             25337..26641
                     /gene="hisD"
                     /locus_tag="J1138_001447"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBA8453264.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSTLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            26638..27708
                     /gene="hisC"
                     /locus_tag="J1138_001448"
     CDS             26638..27708
                     /gene="hisC"
                     /locus_tag="J1138_001448"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBA8453265.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKVVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALQEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            27708..28775
                     /gene="hisB"
                     /locus_tag="J1138_001449"
     CDS             27708..28775
                     /gene="hisB"
                     /locus_tag="J1138_001449"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBA8453266.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLRLQ
                     KAGYKLVMITNQDGLGTQSFPQADFEGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            28775..29365
                     /gene="hisH"
                     /locus_tag="J1138_001450"
     CDS             28775..29365
                     /gene="hisH"
                     /locus_tag="J1138_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBA8453267.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            29365..30102
                     /gene="hisA"
                     /locus_tag="J1138_001451"
     CDS             29365..30102
                     /gene="hisA"
                     /locus_tag="J1138_001451"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBA8453268.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            30084..30860
                     /gene="hisF"
                     /locus_tag="J1138_001452"
     CDS             30084..30860
                     /gene="hisF"
                     /locus_tag="J1138_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBA8453269.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGINSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            30854..31465
                     /locus_tag="J1138_001453"
     CDS             30854..31465
                     /locus_tag="J1138_001453"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBA8453270.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLHQLEQLLAERKSAAPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(31651..32628)
                     /gene="wzzB"
                     /locus_tag="J1138_001454"
     CDS             complement(31651..32628)
                     /gene="wzzB"
                     /locus_tag="J1138_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBA8453271.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKASEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEQDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(32775..33941)
                     /gene="ugd"
                     /locus_tag="J1138_001455"
     CDS             complement(32775..33941)
                     /gene="ugd"
                     /locus_tag="J1138_001455"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBA8453272.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(34190..35596)
                     /gene="gndA"
                     /locus_tag="J1138_001456"
     CDS             complement(34190..35596)
                     /gene="gndA"
                     /locus_tag="J1138_001456"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBA8453273.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         9030 a         9198 c         8973 g         8435 t
ORIGIN      
        1 ttttctcatt agccgattaa gtcagccatt ctttttcctc gctttttttt atagtcatat
       61 cgtcaaaaaa atccaggcga ggtctttatg caacaagaag cgttaggaat ggtagaaacc
      121 aaaggcttaa ctgccgccat agaggccgcg gacgccatgg tgaagtcggc aaatgttttg
      181 ctggtcggct acgaaaaaat aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc
      241 ggggcggtaa aagcggcaac agacgcgggt gccgcagctg cgcgtaacgt cggcgaagta
      301 aaagccgtac acgttattcc ccgcccacat accgatgtcg aaaaaatctt accaaaggga
      361 attaacccat gagcagcaat gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg
      421 tagcgacgcc agagcatgag gttctcccga aaaccactca tcaaatacga gagacggcta
      481 tggcagaaaa aagctgcacg ttaacagaat ttgttggcac cgctattggc gacactgtag
      541 gtctggtcat tgcgaacgtt gatagcgccc tgttggacgc gatgaaactt gaaaaacgct
      601 atcgttccat tggcattctg ggggcgcgta ttggcgcagg gccacacatt atggccgctg
      661 atgaagcggt aaaagccacc aatactgaag ttattagcat tgaacttcct cgcgatacta
      721 aaggcggtgc cggacacggc tcattgatcc tcttaggtgg caacgatgtt tccgacgtga
      781 aacgcggtat tgaagttgcc ctgaaagaac tcgatcggac ctttggtgat gtctatgcca
      841 acgaagccga gcatcttgaa ctgcaataca ccgcccgcgc cagctatgca ctggaaaaag
      901 cgtttggcgc tccactggga cgggcttgcg gcgttattgt cggcgctccg gctgcggtgg
      961 gtgttctaat ggccgacacc gcgctgaagt ccgccaatgt ggatgtcgtg gcatacagct
     1021 cccccgctca aggcaccagc tacagcaacg aagtcattct gattatctcg ggtgactccg
     1081 gcgcagtccg ccaggcggtc atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc
     1141 tcggcgatac accgaaaaac gatcgccctt cctacatctg aattctctga ggcagattta
     1201 tgagatcgaa aagatttgaa gcactggcaa aacgcccggt caaccaggac ggtttcgtta
     1261 aagagtggat tgaagaaggc ttcattgcga tggaaagccc gaacgatcct aaaccgtcaa
     1321 tcaaaattgt taacggcgtc gttaccgaac tggatggaaa acccgccagt cagtttgacc
     1381 tgatcgacca ctttatcgcc cgctacggta tcaatctcgc gcgcgcagaa gaagtcatcg
     1441 cgatggattc ggtaaaactt gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata
     1501 tcgttcccct taccacagca atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga
     1561 acgtggtaga gatgatgatg gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac
     1621 aggctcacgt caccaacatc aaagataacc cggtacaaat tgccgccgac gcagctgaag
     1681 gcgcatggcg cgggtttgat gaacaagaaa ccaccgttgc cgttgctcgc tatgcaccgt
     1741 tcaacgccat cgccctgctg gtcggctcac aggtgggacg tccgggcgtc ttgacccagt
     1801 gttcactgga agaagccacc gaactgaaac tcggtatgtt aggccacacc tgttacgccg
     1861 aaacaatttc tgtctatggt acagaacctg ttttcaccga tggcgatgat accccgtggt
     1921 caaaaggatt tctcgcgtca tcctatgctt ctcgcggctt aaaaatgcgc tttacttccg
     1981 gctccggctc tgaagtacag atgggttacg cggaaggcaa atcaatgctt tatctcgagg
     2041 ctcgatgcat ttacatcacc aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa
     2101 gctgtatcgg tgttccatca gctgtgccat caggcatccg cgcagtatta gcggaaaact
     2161 taatttgctc atcgctggat ctggaatgcg cctccagtaa tgaccagacc tttacccact
     2221 cggatatgcg ccgtaccgca cgtttcctga tgcaatttct gccaggtacc gactttatct
     2281 cttccggttt ttccgctgtg ccgaactacg acaacatgtt tgctggttca aacgaagatg
     2341 ctgaagactt cgatgattac aacgtcattc agcgtgacct gaaggttgat ggcgggctgc
     2401 gaccagtacg cgaagaagat gttatcgcaa tccgcaataa agccgctcgc gctttacagg
     2461 ctgtctttgc cggtatggga ctaccgccaa ttaccgatga agaagtcgaa gcggcaacct
     2521 atgcccacgg ttcgaaagat atgcctgagc gcaatattgt ggaagacatc aagttcgccc
     2581 aggagatcat caataaaaac cgtaacagcc tggaagtggt gaaagccctg gcgcaaggcg
     2641 gttttaccga tgtcgcccag gacatgctca acatgcaaaa agcaaaactg accggcgatt
     2701 atctccatac ctcagccatc atcgtcgatg acggacaagt gctctctgcg gtcaatgacg
     2761 tcaatgatta cgccggacca gctacaggtt accgcctgca aggggaacgc tgggaagaga
     2821 tcaaaaatat tcccggtgca cttgatccca acgaaattga ctaagggtga gaaatggaaa
     2881 ttaacgaaaa attactgcgt cagattatcg aaggcgtgct ggcagaaatg caaaccagcg
     2941 acaagtccgt ttcatttcgc gcacctgtct ctgcaactgt ctcttcagtg ccagataccg
     3001 aaaattttct gaccgaaatt ggcgaagccc aacaaggcac tcagcaagat gaagtcatta
     3061 ttgctgtagg cccggcgttc ggcctggcgc aaaccgtcaa tatcatcggt attccgcata
     3121 aaaacatttt gcgcgaagtg attgccggta tcgaagaaga aggcattaaa gcccgtgtga
     3181 ttcgctgctt taaatcgtcc gatgtcgcat tcgtagcagt cgaaggagat cgtcttagtg
     3241 gttcaggtat tgctatcggt attcagtcga aaggcacaac ggttatccac cagcagggac
     3301 ttcctccctt gtctaacctg gaacttttcc cacaagcgcc tttgttgacg ctggatactt
     3361 atcgtcagat tggcaaaaac gccgcgcgtt atgcgaagcg cgagtctcca caaccagttc
     3421 cgaccctcaa cgatcaaatg gcgcggccta aataccaggc caaatcagcc attttgcata
     3481 tcaaagagac gaagtacgtg gtcactggca aaaaaccgca agaactgcgc gtgacgtttt
     3541 aacaaaggaa accttcatga ataccgatgc aattgagtcg atggtcaggg acgtgttgaa
     3601 ccgcatgaac agtttacagg atgcagctcc tgtttctgcg gtccccagcg cgtcaataca
     3661 gagcgccaaa gtgacggatt acccgctggc aaataaacat ccggaatggg ttaaaacagc
     3721 aaccaataaa acgctcgacg atttcactct ggaaaatgtg ctaagcgata acgtcaccgc
     3781 ccaggatatg cgcattaccc ctgaaacatt gcgtattcag gccgcgattg ccagagatgc
     3841 tggtcgtgat cgcctggcga tgaattttga acgcgccgct gagcttacct cagtaccgga
     3901 tgatcgcatc ctcgaaatct acaatgcgtt acgcccttat cgttcgacta agcaagaact
     3961 tattgccatt gctgacgatc ttgaacggcg ttatcaggcc caaatttgtg ctgcatttgt
     4021 ccgtgaagct gcggaacttt atgtcgagcg caaaaaactg aaaggcgacg attaaggacg
     4081 aattatgcga tatatagcag gcattgatat cggcaactca tcaacagagg ttgcgctggc
     4141 aacgctcagt gcaactggag agttgtcgct cgtgagtagc gccctggcgg aaaccaccgg
     4201 aattaaaggt acgttgcgta acgtccatgg tattcaggag gcgctcgcac aggcaacgaa
     4261 aaaagtcggc atcaatgtta gcgatatttc gcttattcgt atcaacgaag caactccggt
     4321 cattggtgat gtggcgatgg aaaccatcac tgaaactatc atcaccgaat caaccatgat
     4381 cggtcacaac ccgaaaactc cgggcggagt ggggctgggc gtagggctca ccatcactcc
     4441 gcaagagtta ttaacatgcc cggcggacac accctacatt ctggtggtgt catcggcatt
     4501 tgattttgcc gatatcgcaa cgatgataaa cgcctccgta cgagccggat atcagctgac
     4561 tggcgttatt ctgcaacgcg acgatggcgt actggtcaat aaccgcctgg aaattcctct
     4621 acctattgtt gatgaagtcc tctacatcga tcgtattccg ctcggcatgc tggctgcaat
     4681 tgaagttgcc gttcccggga aagttatcga aaccctgtct aacccgtatg ggattgccac
     4741 cgttttcgcc ctcaatgctg aagagactaa aaatattgtt cctgtcgcgc gggcgctaat
     4801 cggcaaccgt tcagcagtgg tagtgaaaac gccttctggc gacgttaaag cccgttcgat
     4861 acccgccgga aatatcgaac tgctttctgc cgggcgcaca atgcgggttg atgtagctgc
     4921 tggtgccgat gccattatga aagcggtcgg tgagtgccca aaactggaaa acgtgacggg
     4981 agaacctggc tccaacatcg gcggaatgct ggaacatgtt cgccagacca tggctgaact
     5041 gaccaacaaa cccagcaacg aaatttttat tcaggatttg ctggcgatag atacctctgt
     5101 ccccgtcaac gtaaccggtg gtctggcagg tgaattttca ctggaacagg ctgtgggtat
     5161 tgcctctatg gtgaaatccg accgtctgca aatggcgatg atcgccagtg aaattaagca
     5221 aaaactgcat gttgatgttc aggtcggagg cgcagaagca gaagccgcca ttcagggggc
     5281 cttgactacc cctggaacga cacgtccact ggcaattctg gacttaggtg caggctctac
     5341 cgatgcctcc atcatcaatc agaaagggga aatagttgcc actcaccttg cgggagcggg
     5401 cgacatggta acgatgatta tcgctcgtga gctgggctta aatgatcgtt atctggcaga
     5461 agaaatcaaa aagtacccgt tggcaaaagt agaaagcctg ttccacttac gccatgaaga
     5521 tggcagcgtt cagttcttcc ctactcccct atcccctcat gtatttgccc gcgtttgtgt
     5581 ggtgaaacct gacgaactgg tgcctattcc cggtgatatg acgctggaaa aagtacgcgc
     5641 tgtccgtcgc agtgcgaaag agcgtgtctt cgtaactaat gccctgcgtg cgctgcgtca
     5701 ggtaagccct gccggaaata ttcgcgacat tccgttcgtc gttctggtag gtggctcttc
     5761 gctggatttc gaagtaccgc aattggtcac cgacgcgctg gcacattatc gactggtcgc
     5821 cgggcggggg aatatccgtg gtagcgaagg tccgcgcaat gcggtagcta ctgggctcct
     5881 catcgcctgg cacaaggagt cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg
     5941 tcatggagat cggtgactgt atcactacat gggatgaagt gcttcttggc attgaagagg
     6001 aaggtattcc ctttcgcatt caacacattc cgtcaggtga agttatcgat agcgcctggc
     6061 tggctgctcg tcaatctccc ctgttggttg gcattgcctg cgatcaggaa aaactgattg
     6121 tgcattacaa aaacttaccg gcatcagcgc cactgtttac gttgatgtat caacaagata
     6181 accatgccag gcgcagcatc ggcactaacg ctgcccgcct ggtaaaaggg attcccttcc
     6241 gggaactcca ctcataatcc acaggagaaa cgcagtatga acaacgcact aggactggtt
     6301 gaaacaaaag ggttagttgg cgcaattgaa gctgcagatg ccatggttaa atccgccaac
     6361 gtgcagctgg ttggctatga aaagattggt tcgggtctga ttacggtgat ggtccgtggc
     6421 gatgttggcg cagtaaaagc cgccgttgat gcaggaagcg ccgccgcaag cgccgttggc
     6481 gaagtgaaat cctgccatgt catcccgcgc ccacacagtg atgttgaggc tattttacct
     6541 aaatcagcat aaccccattc gctaaggagc acagcgtgaa gcaatcactg ggattacttg
     6601 aagtcagtgg tctggcactc gccatcagtt gcgcagatgc tatggcaaaa gccgcgtcca
     6661 tcacgctggt tggccttgag aagaccaatg gctcaggctg gacgctcatc aaaatcaccg
     6721 gtgacgtcgc ctcggttaat gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa
     6781 atggcctcgt cgcacacaaa gtgattgccc gtccgggaga cggcattctg ccccacacaa
     6841 ctgaagcatt gactgtgacg gttgaaacaa gcgaaacacc agcaacaacc gtcatctcag
     6901 agattatcag ttgtaatttg tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc
     6961 gctcactttg cctgcacgcc aataagcgag gggagtaaca gtggataaac aaaccttgca
     7021 ggaaacagtg agccagattc tggatgagat gcgcgagcgc cctatccctc tgggtgtttc
     7081 cagtcgtcat attcatcttt caacagcgga ttatttgcgt ctttttcctg aacaaccgat
     7141 tagcgagaaa aagctgttaa tgcaacctgg ccaattcgct gctgaacagg ttgttacgct
     7201 ggagggacca aaaggtaaat tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag
     7261 ccaggtggaa atttcccgta cggacgccag aacgttaggg atcaccgccc cgctacgcat
     7321 gtcaggcgat ctcagcggca ctccagggat tcgtttgata agcccatttg cggaactgga
     7381 gatcacatct ggcgtcattg tcgcacaacg gcatattcat atgtctccgc tggatgccct
     7441 gatttatggc gttacgcatg gcgataaagt ggcggttgcc attaaaggtg atgagcgacg
     7501 actgatattc gaaaacgtcg ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat
     7561 tgataccgat gaagccaacg ccgcaggggc tgatagccct gacgtttttg ccacactggt
     7621 gtcagtgcca tgaataacat gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt
     7681 aaacaacgtg cgggaaaaac actcgtgctg actgtttttc aactgcgaga cgccagcgtt
     7741 caagagatag ttcatcagta tgcatcatta caaatccgat atgtcgatct gccgctgcta
     7801 cgacaacttg cggggaacga aacgtcagat cgagcagcaa ttcagataca cgaagcgtta
     7861 gcctggggat tgcacattca attgtcgttg cagtgtcatt tcctgaacgc cattgaattg
     7921 aagactctcg cccgtctacc gctaagctgg tgcgacgagc aagggcaacc gatctacctt
     7981 catcgtgatc gacttttaag ttatgccgat attgcacagt taagctcagg aatactggtg
     8041 ttacagcgaa aatgttgtgt gaccgcactg gctcgtgaag cggcaattac ccgaaatatt
     8101 cagttaatca ggcaggagta acctatgcat ctggctcgag tcactggcgc ggtagtatcc
     8161 acgcaaaaat caccttctct gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct
     8221 gacggcgaac tgcctctcac gccattaacc ggcgacgaag ttgccgttga ttccgtcggc
     8281 gcaggcgttg gtgagttggt actcctcagc agtggttcca gcgccagaca tgttttttcc
     8341 ggccctaacg aagccatcga cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa
     8401 ggagttgaaa gatggcaatt tatacccgaa caggtgattc aggaagtacg tctttattta
     8461 ccggacagcg cgtcagcaaa actcatctac gtgtcgaaac ctatggcacg ctggatgaac
     8521 tgaacgcgac actcagtctg tgttattgcg ccaccgccat cgaaagccat cgcatcttac
     8581 tggaagccat tcaacaacag atattttggt ttagcgctga actcgccagt gaaagcgaac
     8641 agccgtcagc gcagctgcgt tacatcggta cggaagaaat tgccgcgctg gaaaacgcga
     8701 tcgatagcgc aatgaacgcc gtccctccag ttcacagctt cattctgcct ggtcgatgtg
     8761 aagccgcaag ccgcatgcat ttcgcccgca cggtggctcg acgggctgaa agacgtctgg
     8821 ttgaactgac aacggaaacc atcgtccgga atgttttgct gcactatatc aaccgacttt
     8881 ccgattgcct gtatgccctc gcccgcgtgg aggacaatgt tgctcatcaa aacctgatga
     8941 ttcaggaaat cacaaaacgt tatcacgaag ccaaccacac accagcattg aaggaacgca
     9001 caatgccgct cacttttcag gatcttcacc agctcattcg tagtgcggca atgcgcgcag
     9061 atgaactgca tattcccgtt gtcatcagca ttgttgatgc caacggaaca gaaagtgtta
     9121 cctggcggat gcctgatgcc ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga
     9181 ccgccgtggc gatgaaaacg gcaacccaca aacttgctga taccgtgcaa cccggcgcgc
     9241 cgctttacgg gctcgaaagc catatgcaag gcaaagtcgt cacttttggt ggcggcttcc
     9301 ccctctggcg tgacggaaaa ttgcttggcg ggcttggcat cagcggcggt agcgttgaac
     9361 aagacatgga tattgcccaa agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa
     9421 taactctgaa ttagaaacac tgatccgcaa tattctgcgt gaacaacttg tccccgctac
     9481 gccacgaatg caaagaaacg ctatttttca gactgttgat gaagcggtct gcgcagcaca
     9541 ccaggccttt ttacgctttc aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc
     9601 cattcgcgaa gaattaatgc cccgccttac agaactggca gaagaaagcg ccagtgaaac
     9661 agggatggga aataaagagg ataagtacct aaagaacaaa gcagcactca ataatacgcc
     9721 aggaattgaa gatttaacca ccactgcact aaccggtgat ggtggcatgg ttttgtttga
     9781 atactccccg tttggggtta ttggctcggt cacgccaagc accaacccca cggaaaccat
     9841 cattaacaac agcatcagca tgctggcagc aggtaatagc gtctacttca gtccacaccc
     9901 gggagccaaa tacgtttcgt taaaactgat taccacaatc gaagacatcg cgtttcgctg
     9961 tagtggcatt cacaacctga tcgttaccgt atctgaacca acctttgccg ccaccgaaca
    10021 gatgatggcg caccctaaaa tcagcgttct ggcgattacc ggcggcccgg ggatcgtcgc
    10081 catgggaatg aaaagtggca agaaagtgat tggtgccgga gcgggtaacc caccctgcat
    10141 tgtcgatgaa acagcggata ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt
    10201 tgattacaac ctgccgtgta tcgctgaaaa aagcctgatt gtcgtggcat cagtggcaga
    10261 ttatttaatt cagcagatgc aaagctttgg cgcacttctt cttaaccatg aacaaacgga
    10321 gaaattgcgt gcaatttgcc tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag
    10381 cccatcagcc ttgctggaag cagcgagcct ccctttgccc gcaaaagcgc cgcgcctact
    10441 gattgctgtt gttgacgccg atgatagttg ggttacgtgt gaacaactga tgccaatgct
    10501 gcccattgtg aaggtcaacg attttgacag cgctctcgcc ctggcgttga aagtggaaga
    10561 cggcttgcac cacacggcaa ttatgcactc gcagaacgtc tcacggctca accttgccgc
    10621 gcgcgttatg caaacctcaa tctttgtcaa aaacggcccc tcctgggcgg gtattggcgt
    10681 cggcggcgaa ggctttacga cctttaccat tgcaacaccg accggagagg gtacaacgtc
    10741 agcacggact tttgcccgct cacgacgctg cgtgttaacc agcggctttt ccattcgcta
    10801 gagaaacgga aatgaaaact ttttcactgc agacgcgttt atacagcgga acgggaagtc
    10861 tcaacatact gagccgtttc acgaataggc acatctggat tatttgcgac gtctttctgg
    10921 cacactcacc actcattgac acgttgcatc aggcgctgcc cgacagtaat cgtctcagta
    10981 tttttagcga aataacgccc gacccaacta tccagaccgt ggtgaaaggc attgcgcaaa
    11041 tgcagacttt acgcccggat gtggttatcg gttttggcgg cggttctgcc ctggacgccg
    11101 caaaagcgat tgtctggttc ggtcgccagt gtggcattga aattgaaacc tgcgtagcca
    11161 ttcctaccac cagcggtacc ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg
    11221 agaaagggat taaatatccc cttttcgata atgcacttta tccggatatc gcgatccttg
    11281 atccatcttt ggtcgtcagc gtgccaccag cgataacggc caataccggg atggatgttt
    11341 taacccatgc gcttgaagct tatgtttctc cccgcgccag cgacttcacg gatgcactgg
    11401 tcgaaaaagc cgtgcaaatt gtttttcaat acctgcccac tgcagtgaag aaaggcgatt
    11461 gtcttgccac ccgtggcaaa atgcataatg catcgacgct tgcaggaata gcctttagcc
    11521 aggccggact cggtttaaac catgcacttg ctcatcaact cggcggacag tttcatctgc
    11581 cgcacggcct ggcgaatgcg ctcctgctca cggcggtaat ccgctttaat gctggcgatc
    11641 cgcgcgcagc caaacgctac gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg
    11701 ccaatgacac cgccagcctg aatgcactta ttcaacatat tgaacagctt aaaacaacct
    11761 gtacgctgcc aacacttgcc aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca
    11821 taccagatat ggttcaggca gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcgg
    11881 ctgatgcctc cgcgattgca gaactgctcg aggagttgtt atgaacgtcg agttaacctc
    11941 ctgcgatgac cagaccatcc gcgagcgagt acaagcagca ggtgttgtgg gtgctggcgg
    12001 cgcaggtttt ccaacccata ttaagctaca agcgcgtgtt gacacgttcc tggtgaatgc
    12061 ggcggaatgc gaaccaatgt tgaaagtgga tcaacagtta atggtgcagc aagccagccg
    12121 cctgcttcgc ggtgttcact atgctatgaa agccaccggg gccagtagcg gaatcatcgc
    12181 gctgaaagaa aaatatcaac gggcaataaa cgcactcaca ccactgttac caccggatat
    12241 ccgcctgcat attttgcccg atgtttaccc cgcaggagac gaagtgctga ccatctggat
    12301 ggcaactggt cgccgcgttc cacccgctgc attacccgtt agcgtcggtg tggtggtcaa
    12361 taatgttcaa accgtactta atattgccag agccgtcgag caacagtatc cagtgacgcg
    12421 ccgcacactc actattaatg gtgcagtggc gaaaccaata acggtaaccg tccccatcgg
    12481 catgtcttta cgtgaagtcc tggcgctggc gggcggcgca acagtagatg ctcctggttt
    12541 tatcaatggc gggccgatga tgggaagttt gatcacatcg ctcgacaccc ccgtcagcaa
    12601 aaccactggc ggattgctgg ttttacccaa ctctcattca ctgatccaac gtcgaatgca
    12661 gaatgaccgc agcgtacttg ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac
    12721 cgatctctgc ccacgtcatt tagtcggtca cgaactggcc ccccatttat tagtacgtgc
    12781 ggttaactac caacagctcg ctacgccaca actgttactg acggcgctca cctgttcgga
    12841 gtgcaacgtc tgcgccagcg tcgcctgtcc ggtaggaatt tcaccaatgc gcataaaccg
    12901 cctgcttaag caggaattac gcgcacaaaa tctccgctac gaaggtgcct taaaccctgc
    12961 tgatcccatg gcaaattatc gactgattcc cgtaaaacgg ctggtaacca gacttggctt
    13021 aaccccgtgg tatcaggatg cgccattgtc tgaacaggtg cctcagaccg aaaaagtgac
    13081 gttattgtta cgccagcaca tcggtgccag cgcaattgcg tgtgtacaaa aaggcgatcg
    13141 cgtcgttcat gggcaatgtg taggtcaaat tccacacggc acattaggtg cccccattca
    13201 tgccagtatt gatggcatgg tcagcgatgt gacagaaaac gcaattacgc ttgtgagagg
    13261 ttaacgatgt cgaaagcggt aggtatttta gaacttagca gtatcgcgaa agggatggaa
    13321 accggcgacg ccatgctgaa aagcgcaaac gttgagttac tggtcagcaa aacgctatgt
    13381 cccggaaaat ttctgctgat gctcggtggc gatgtgggtg cggtgcaaca ggccatcaac
    13441 agcggtgctt cccttgctgg tgaaatgctg gtcgacagtc ttgtcctgcc gaatattcat
    13501 cccagtctcc tttctgcaat cagcggactg aaccatgttg agcagcaccg ggctgtcggc
    13561 gtagtcgaaa cctggagtgt cgctgcctgc attgacgctg ccgattgtgc tgtcaaagct
    13621 gcgaatgtca ttctcgttcg cgtgcatatg gcttttggca ttggcggaaa atgctacatg
    13681 gtggttgctg gcgatatttc agatgttaat aacgcggtct ctgtcgccag cgagcgtgcg
    13741 ggagaaaaag gtttgttggt ctatcggtcg gtgattgccc gtccacatga agccatgtgg
    13801 cgtcagatgg tggagggata atgacaactc agcacacaac tgaacggatg attcaggaat
    13861 atgtccctgg taagcaggtc acgctggcac atgtcatcgc caatcctggg aaggatctgt
    13921 ttaagaaatt gggtttacca gacaccgttt ctgctattgg cattctgacg attaccccca
    13981 gcgaagcatc tatcatcgca tgcgatatcg ccacaaaatc aggcgctgta gagatcggtt
    14041 ttcttgatcg ttttaccggt gccgtagtgc tgactggtga tgtttctgcc attgaatatg
    14101 cacttagcca ggttacccgt acgctgggtg aaatgatgcg ctttaccgcc tgccccatta
    14161 cgcggacctg acgttatgca acgtatgatg cttatcggcc ccagccagtg cggaaaaacg
    14221 tcactcacgc aatgtctgcg tggcgaaaat atttattatc agaaaacgca ggctatcgtc
    14281 tggtccccgg ccaccattga tacacctggc gaatacctcg aaaaccgcag cctttacagt
    14341 gcattgttgg tcagtgcctg cgaagcagac attatcgcgc tggtacttaa tgcggacgca
    14401 ccgtggtcac ctttttcgcc aggctttacc tcgtcaatga atcgcccggt cattggagta
    14461 gtaaccaaag cagatttagc cagtatggaa caaatatctt tggtgaaatg ttggttacgg
    14521 gaagctggag cgcacaacgt gttagtaacc agtgcggtta aaaacaatgg agttacagaa
    14581 ctttttgcct tgctgcatac agaagaaggc tgtcgctaat ttcgataggt tctcagatga
    14641 gaatgatgac ggtgataaca gataagaatc aatgagcaat gagggttgcc gggcaaccct
    14701 cattgaataa aacaggaatt acttcgcttc gccgtttttc atttcgccca tagctttcag
    14761 ctttttggag atatcgcggc gttctttgga gagctcggca tttttgatga tgtaatcgtc
    14821 aacgcgatct tcatagtcac ctttcatgct ggcgatgatg ccctggattg cttcaacgct
    14881 catccctggc ttgatgtaat cgctcaggtt gtccagcaac aggacgcgct tctggttgtc
    14941 acggatcttt ttctcaacgt cctgaatttc acgttgcagt ttgttcttac gacggaacag
    15001 acgaacaaat tccagtacgt cctggaatga aggcttggta gtttccattt ttatacccct
    15061 gataatgtga gagtcggatt cgtttaagca accgctattc gttagggcca acattactca
    15121 tagctgccgc agatgacaat gcttttatcc cttttcacta tcataccctt tatccttgct
    15181 gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca
    15241 aatcagattc gatagcaact caagctgatg tgccgtttcc atgttcagcc acacataacc
    15301 atagattggt gtttccacaa ccttcaggtc atggtggtta tcgagcaact gacgcagctc
    15361 ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta
    15421 cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat cacgaagttt
    15481 ttgcgcgttc aataacacga aatggctggg gcgcgtggcc cagtatgcat tgatttgcaa
    15541 ctccagcata caaaccagat tgcgattaat ggtctggata ccttcatata tcgatttggg
    15601 aatacgggtt tctttgctgg cgggcgcaat cagcccacgc attttcacgg catcggtcag
    15661 gagtttttgt agatggcttt ccaaacgtgg gcgttcgagt aagttcggtg agaatgcaga
    15721 ttgatagacc cgattatact cggtcagact tttcgccagt tgaatgcgcc agtggatgaa
    15781 cgcccgttgt ggccagatac cggtaaacaa cattgccagt aaagagccga ggatcacatc
    15841 gccgcttcgc cataacgccg tatcaatttc acctgtcggg gaacccacaa caattgccag
    15901 cgtcacccca atcaataatc cttgatacgg tttcttgccc agcgccagcc aaccgcaaag
    15961 aaacatcgcc gccgcgcacc agactaacat cagcggtaac gaaattaact ccagttgcag
    16021 agcgataaga cctaaaatcg aacctaacac cgtaccgcca atacgttcga aggcacgagg
    16081 gacaacgttc ccccagaacg agattggccc cataatcacc accatggtga ccagcggcca
    16141 ggtgctttcc gggatagtaa acaggcggat aatgagaaaa gtgagcagga atgccagcgc
    16201 gacccgagta ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa acgggcttaa
    16261 tgacttatcg gcacgcacgc agaaactccg taatgaagat taaaagcgct cataacacat
    16321 ctgtcggcca gaaggcgcgc ctctgagtta tccgacggac ataccgggaa cagtgtaatt
    16381 tcccggcagg agcgaaaaat ttgaaaggcg catcttactc tttccgcttt caaaaaaaga
    16441 gtggtcttcg cgttaacaca ccgccctgag atgaattagt gatgttttgg ctgcaccgca
    16501 acatacattt cgatatccca gtacccatct tccgcgccat tgttcaagta aacctcgaag
    16561 cagggctttg gtaacatttc ataagcattg tcctgcaaga ggctattaaa gaactgatac
    16621 cagggtttag caaaatcatc accgactaca cgagccaccg ccaccgcata ctgaccacct
    16681 gaaatttctg tcagaatgac gccctcactg ttttcgggga gcgtaaagtt attcggtacc
    16741 gtcacgacgg tgtcgcagcg taatttttcg gcgggtgttt catctggatt gtcgtaatag
    16801 acagcaaccc actccttcgg cacaatattt ttgctatcta tccacatcat caactgctca
    16861 aaacctttct ttaccgtctg ctcccacggg ccaacaagat ggaaacctgc aacggtacgt
    16921 ttatcttcct gcttaatctc gtagttcatg acgcctccac taacactgtt tttatataca
    16981 gtatagttgc aatattaaaa ccacaaggaa tgagtgttga ttatgcgagc agactcgcac
    17041 tcctgccagt ctgctgcaaa agaaaggtca ggccttatgg tggaaataat cactcaggcg
    17101 ggaaaacatg ctgccttccc cgacagattc cagggtaacc agcggccagt gcgccacctg
    17161 tttatcacgg tcgtaaagtt caatttcccc tacccgctga tgggcgctaa ttggcgcggt
    17221 gagttcttta ccatcaaggg tatatttggc tttgatatgt ggaatttcgg ctttcggtag
    17281 caccatccag aactcttgtt ccgttcccag ggcgatattt tctttatcgc cataccagat
    17341 gcgttccgta ccgacctttt tcccacggtg caaaatttgc accgtagtaa agttttgttg
    17401 cccccaacgc agtaattttc ttgcctcttc ctcacgacct tttgcactgt cagcacccat
    17461 taccactgca atgaggcgac gctgcccgtc tacagccgaa gcaataagat taaacccggc
    17521 accagaagta tggcccgttt tcaggccgtc aacattcatg gttttatccc acaataaccc
    17581 gttacggttt tgctgggtga taccgttcca ggtgaggctt ttctcactgt acatatgata
    17641 aaactcgggc tcgccgtgga tgatagcgcg agaaagcacg gctaaatcat aagccgagct
    17701 atgctggcca ggtgcatcca gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag
    17761 cttctcggca tagttgttca tcatttcaac aaactgccgt tgcccaccgg caatatagtc
    17821 agccagagca acacaagcgt catttccgga atccacaatt aaaccacggc ttaaatcacg
    17881 taccgatacg cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt
    17941 atctttcgcc cacgcatcac gccccacggt gacaatatcg tctggcgtaa tgcgatgact
    18001 atcgatagcg cgatccacga catagcccgt catcagcttt gtcaggctgg cgggattgcg
    18061 ctgttgatgc tcattacccg cggtgagaat ctgaccggtg gtgtaatcca tcaataccca
    18121 ggacccggca tgaatctctg gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc
    18181 agacgataaa ttaaaaacga acaaagaagc agcaataata agacggcgtt tcaacagcaa
    18241 accctcaggt gtttcaaata gctgttcttt ttacggaaat acttatgaac tggctggaat
    18301 aaagtgcaag aaaatgtgac tactatctca tttttatctg acatgatctg ttgccactcg
    18361 ctgccaaatt gtcgcgctaa agctgattag cacggtgata tttgatactc tggcagacag
    18421 cagaaataac ggatttaacc taatgatgaa tgacggtaag caacaatcta cctttttgtt
    18481 tcacgattac gaaacctttg gaacgcaccc cgcgttagat cgccctgcac agttcgcagc
    18541 cattcgcact gataacgaat tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc
    18601 cgcggatgac tatttacccc agcctggagc agtattaatt accggtatta ccccgcagga
    18661 agcacgggcg aaaggagaaa acgaagccac gtttgccgcc cgtattcact cgctttttac
    18721 cgtaccgaag acctgtattc tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg
    18781 caacgttttt tatcgtaatt tctacgatcc ttacgcctgg agctggcagc atgataactc
    18841 gcgctgggat ttactggatg ttatgcgtgc ctgttatgcc ctacgcccgg aaggaataaa
    18901 ctggcctgaa aatgatgacg gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa
    18961 tggtattgaa catagcaacg cccacgatgc gatggctgat gtgtacgcca ctattgcgat
    19021 ggcgaaactg gtaaaaacgc gtcagccacg cctgtttgat tatctcttta cccatcgtaa
    19081 taaacacaaa ctgatggcgt tgattgatgt tccgcagatg aaacccctgg ttcacgtttc
    19141 cggaatgttt ggggcatggc gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc
    19201 tgaaaatcgc aatgccgtaa ttatggtgga tttggcagga gacatttcgc cattactgga
    19261 gctggatagc gacacattgc gcgagcgttt atataccgca aaagccgatc ttggcgataa
    19321 cgccgccgtt ccggttaagc tggtacatat caataaatgt ccggtactgg cccaggcgaa
    19381 tacgctacgc ccggaagatg ccgaccgact gggaattaat cgtcagcatt gcctcgataa
    19441 cctaaaaatt ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtggcga tattcgcgga
    19501 agccgaaccg tttacgcctt cagagaacgt ggatgcacag ctttataatg gctttttcag
    19561 tgacgccgat cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta atttaccggc
    19621 actggatatc acttttgttg ataaacgaat tgaaaaactg ttgttcaatt atcgagcgcg
    19681 caacttcccg gggacgctgg attatgccga gcagcaacgc tggctggagc accgccgcca
    19741 ggtcttcacg ccagagtttt tgcagggcta cgctgatgaa ttgcagatgc tggcacaaca
    19801 gtatgccgat gacaaagaga aagtggcgct gttaaaagcg ctttggcagt acgcggaaga
    19861 gatcgtctga aacaaaaaag ccggagcgac aaactccggc tttttgagat ttaccggatg
    19921 attatgtcat ccggtttgta agattacgct acgtcttcgt actgcggaac cgggttacgg
    19981 aaactcttgg ttacgcaagc caggtaaatc agaccgatag cagcccagat cagacccaga
    20041 accattgagc tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc
    20101 ggcaggaaca gatactggaa gtgatctttc agcgttttgt tacgcttctc acggatccag
    20161 aactgcgaga tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc
    20221 agcgccgtag ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg
    20281 atgatgttca tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg
    20341 aacacgccgt cacgacccat tacgtacatc agacgcgcaa cgcctgcgtg cgccgccata
    20401 ccggacgcca gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc
    20461 gcaacgtaca gcatgatttc aggctgtgac gcatccggat ctttaaaacg agagatatcc
    20521 gggaagtaca gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc gatcagcgcg
    20581 gtcaggaaaa tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg
    20641 ctgatgccgt caaaaccggt aaaggagaag cacaggattg tcgccccggt aatcatcggg
    20701 ataacatgcg catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca
    20761 ccttcaaata cgccataaac caccatgccc agaatcaccg cgatcagcac tatctgcaac
    20821 acgacgatta cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc
    20881 ataaaggcca ccagcgccac cacaaacatc cacgatggaa tggaaggcac cagagcttca
    20941 aaatagattt tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa
    21001 gaccaaccca ccataaagcc gacagtcgga ctaatggatt tctgggcgta agtgtatgca
    21061 gagccagcag aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc
    21121 gcaatcaatg cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata
    21181 ccaaatgtat caaacagcgt catcggctgc atataggcta gacccatcat tacaaccgga
    21241 actaacgtaa gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac
    21301 atggtcattc tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt
    21361 ggattcctca gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg
    21421 gcctcttggt atgaatgatt ggtttgaagc aaaaaataac cgacgctaat taaacgtcgg
    21481 tttttagtca ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga
    21541 atgagaggct tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg
    21601 cataaacacc ggcgatcgca ctatttgcta aaatttcatc tcgccaccag ccgctggcaa
    21661 gccctgcggt ctgttcattc cagccaatcc agacaggttc aaagagagtt tgggcgacca
    21721 ctttcttctc gattaacgcg ccactatcga gaaaacgttg cgccagataa cggggtaaat
    21781 agccgcagcc aaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa
    21841 tggcttcttg ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc
    21901 ccaccacaat cgcccgatat cgtttaatga tacgacggtt taacggttct tcttcctggg
    21961 ctaacggatg atgaggcgcg accgcaaaga cttgttcaag atcgcccaac cgcgagaaac
    22021 caaactcact ggaagatggt ggctcatgca tcgcgccaac gataatatcg gctcgcccct
    22081 gagtcagggc atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc
    22141 tatgatgttg ataaaaagct tcaatgagag gcgcaagcag agaaaaagga aacgtgtcgt
    22201 ccacgccaat caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca
    22261 gttctcgcac ggtatgcaga acttcccgcc ctttctctaa taacattttt ccggtacggg
    22321 tgaatttagc gcggtgtccg ctacgatcaa gcaattgaat attaaggtcg ctttccagct
    22381 tgtgaacggt gtaactcaga gcggacggcg tcttgtagag tttggctgac gccgccgcaa
    22441 aactgccttc tttttctaag gcatcgagga tcatcaacac atccagcagt ggtttcatac
    22501 acgccccctt gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta
    22561 ccagcggatc gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa
    22621 taatcttacc tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta
    22681 aacgggccat ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat
    22741 agatgtgtcc gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta
    22801 aatggactaa attaacacca tgttcaccat caggggcggt ttttccggca aagaagcgtc
    22861 cgggatgacg tcccggtccg accaggcccg caagacgcag aatatcgacc gaagtaccgg
    22921 gtaaattgtg cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat
    22981 tacgcggggt ggtttctttc accgtgcctt gcgcgtcgcc atagacagat gtcgagctgg
    23041 taaaaataat gcggggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt
    23101 gtaaatagaa ctcatcgcca gggccgctac gacgtgccgg aagcgtaatg accagcgcat
    23161 cggcatccat cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa
    23221 gcagatagct atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac
    23281 tcccggtgac ttgccagcct cgcgctgaaa gtgacatcgc cagcggcata cctaaccacc
    23341 ctaacccgac aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa
    23401 ggctacgcta gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga
    23461 taacgacatg ctgcaatgag cagtgaatca tcagtaaccg cgtataccag acggtgctcc
    23521 tctgtaatgc gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggtttcccc
    23581 ttaccttcaa atggtgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt
    23641 cgcttatctg tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt
    23701 ttcactcaat aatgtccttt tccgttcctt tgcctgattt caggctatcg attgagtcca
    23761 tcaatctccg ggcgttagcg ggggaacgca gtagataagc cgtctcttcc agcgagttgt
    23821 attcttcgag tgacatcaga acacaagcct ctccattctg acgagtaatg aggatcgggg
    23881 catgatcttc aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc
    23941 taattgtacg catgtaaatc tcctcttttg tacagttcat tgtacaatga tgggtgttaa
    24001 ttaactattt attaattagt ttgtagatca agacattgtc agcgagacga aaaatcagac
    24061 tttgctcttt ttggtgctgt caagttagag gacagtcctc ttagccccct tctttccccg
    24121 ctcattcatt aaacaaatcc atcgccataa aatatataaa aaagcccttg ctttctaacg
    24181 tgaaagtggt ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac
    24241 acgcgttcaa tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg
    24301 tgtgctggaa gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa
    24361 gatcaccttc cgggggcttt tttattgcgc gattgataac ggttcagaca ggtttaaaga
    24421 ggaataagaa aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt
    24481 taagtgatga ctcacgcgaa ttactggcgc gctgtggcat taaaatcaat cttcacaccc
    24541 agcgcctgat cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg
    24601 acattcccgg tctggtgatg gacggcgtgg tagaccttgg gattatcggc gaaaacgtgc
    24661 tggaagaaga gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccctgc
    24721 gtcgtctgga tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg
    24781 acggtccgct ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc
    24841 gttatctcga ccagaaaggt atctctttta aatcctgctt actgaacggt tctgtcgaag
    24901 ttgcgccgcg cgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc
    24961 tggaagctaa cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgcctgatcc
    25021 agcgcgatgg cgaaatggaa gaatctaaac agcaactgat cgacaagctg ctgacccgta
    25081 ttcagggtgt gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac
    25141 gcctggatga agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg
    25201 cgggtgatca acagcgcgta gcgatgcaca tggtgagtag cgaaaccctg ttctgggaaa
    25261 cgatggaaaa actgaaagcg ctgggtgcca gttcaatcct ggtcctgccg attgagaaga
    25321 tgatggagtg atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga
    25381 gcaacaacgc cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac
    25441 cgttaacgat attctcgata acgtgaaaac gcgtggcgat gaagccctgc gtgaatacag
    25501 cgcgaagttt gataaaacca cggttaccgc gctgaaggtg tctgctgatg agatcgccgc
    25561 cgccagcgaa cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaagaatat
    25621 tgaaaccttc cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt
    25681 gcgttgccag caggttacgc gcccggtggc ttcagtgggt ttgtatattc ctggcggctc
    25741 cgccccgctc ttctcaacgg tattaatgct ggcaactccg gcgcgtattg cgggctgtaa
    25801 aaaagtggtg ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct
    25861 gtgcggtgtg caggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt
    25921 tggtacggaa tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac
    25981 cgaagcaaaa cgccaggtaa gccagcgtct ggacggtgcg gcgatcgata tgcccgcagg
    26041 cccgtcggaa gtgctggtga ttgctgacag cggcgctacg ccggatttcg tggcttctga
    26101 tttgctttct caggctgaac acggccctga ctcacaggta attttactga cccccgacgc
    26161 cgatatggcg cgtcgcgttg ccgaggccgt cgaacgccaa ctggcagaac tgccgcgtgc
    26221 cgaaaccgcc cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca
    26281 gtgcgtggag atctccaacc agtacggccc ggagcacctg atcattcaga cccgcaacgc
    26341 ccgcgaactg gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc
    26401 ggaatcggca ggcgactacg cctccggcac caaccacgtt ctaccgactt acggctacac
    26461 cgccacctgt tccagcctcg ggctggcaga tttccagaag cgcatgaccg tacaggaact
    26521 gtcgaaagaa ggcttttcca cgctggcttc gaccattgaa acactggccg ccgccgagcg
    26581 cctgaccgcc cacaaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg
    26641 agcaccgtga ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag
    26701 tcagcgcgtc gtctgggcgg taacggcgat gtctggctga acgccaacga atatcccact
    26761 gccgtggagt ttcagctcac tcagcaaacg ctcaaccgct atccggaatg ccaaccgaaa
    26821 gtggtgattg aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagc
    26881 cgtggcgcgg acgaaggcat tgaactgctg attcgcgctt tttgcgaacc gggtaaagac
    26941 gccatcctct actgcccgcc aacgtacggc atgtacagcg tcagcgctga aaccattggc
    27001 gtcgagtgcc gcacagtgcc gacgctggac aactggcaac tggacttaca gggcatttcc
    27061 gacaagctgg atggcgtaaa agtggtctat gtttgcagcc ccaacaaccc caccgggcaa
    27121 ctgatcaacc cgcaggattt tcgcaccctg ctggagttaa cccgcggtaa ggcgattgtg
    27181 gttgccgatg aagcttatat cgagttttgc ccgcaggcat cgctggctgg ctggctggcg
    27241 gaatatccgc acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcggggctt
    27301 cgttgcggat ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc
    27361 ccctacccgc tctcgacgcc ggttgccgac attgccgcac aggcgttaag cccgcaggga
    27421 atcgtcgcca tgcgcgaacg ggtggcgcaa attattgcag aacgcgaata cctgattgcc
    27481 gcactgcaag agatcccctg cgtggagcag gttttcgact ccgaaaccaa ctacattctg
    27541 gcgcgcttta aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta
    27601 cgtgatcaga ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt
    27661 gaagaaagcc agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt
    27721 atctttttat cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg
    27781 accgttttga caaactcgcc tttgaacctg gcgtgatccc ggagctgctg aggctgcaaa
    27841 aagcgggcta caagctggtg atgatcacta atcaggatgg tcttggaaca caaagtttcc
    27901 cgcaggcgga tttcgaaggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg
    27961 tgcagtttga tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta
    28021 agccgaaagt aaaactggtg gagcgttatc tggctgaaca agcgatggat cgcgccaaca
    28081 gttatgtgat tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg
    28141 gtttacgcta cgaccgcgaa gccctgaact ggccgatgat tggtgagcaa ctcactaaac
    28201 gagaccgtta cgcccatgta gtgcgcaaca ccaaagagac gcaaattgac gtccaggtgt
    28261 ggctggatcg cgagggtggc agcaagatta acaccggcgt tggcttcttt gatcacatgc
    28321 tggatcagat cgccacccac ggcggtttcc gtatggaaat caacgtcaaa ggcgacctct
    28381 atatcgacga tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa
    28441 ttgcccttgg cgacaaacgc ggtatttgcc ggtttggttt tgtgctgccg atggacgaat
    28501 gccttgcccg ctgcgcgctg gatatctctg gtcgtccgca cctggaatat aaagccgagt
    28561 ttacctacca acgcgtgggc gatctcagca ctgaaatgat cgagcacttc ttccgttcgc
    28621 tctcttacac catgggcgtg accctgcacc tgaaaaccaa aggtaaaaat gatcaccacc
    28681 gtgtagagag cctgttcaaa gcttttggtc gcaccctgcg ccaggccatc cgcgtggaag
    28741 gcgacactct gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgatactgg
    28801 ctgcgccaat ttgaactcgg tgaagtcagc cattgcgcgt cacggttatg aacccaaagt
    28861 cagccgtgac ccggacgtcg tgttactggc cgataaactg tttttacccg gcgttggcac
    28921 cgcgcaagcg gcgatggacc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg
    28981 tacccaaccg gtgctgggca tctgcttagg gatgcaactg ctggggcggc gcagcgaaga
    29041 gagcaacggc gtcgatttac tgggcatcat cgacgaagac gtgccgaaaa tgaccgactt
    29101 tggtctgcca ctgccacata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct
    29161 gtttcagggg attgaagacg gcgcgtactt ttactttgtt cacagctacg caatgccggt
    29221 caatccgtgg accatcgccc agtgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa
    29281 agataacttc tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaagtt
    29341 gctgaaaaac ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc
    29401 gtggtgcgtc tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg
    29461 ccacgcttac aggattacgc tgcgcaaggt gccgaagtgt tgcatctggt ggatctgacc
    29521 ggggcaaaag atccggctaa gcgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt
    29581 aacgtcccgg tgcaggttgg cggcggcgtg cgtagcgaag aagacgtggc ggcgttactg
    29641 gaagcgggcg ttgcgcgcgt agtggtcggc tccaccgcgg tgaaatcacc agaaatggtg
    29701 aaaggctggt ttgaacgctt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt
    29761 gacgagcaag gtaacaagca ggtggcggtg agcggctggc aagagaactc gggcgtttca
    29821 ctggaacaac tggtggaaac ctatctgccc gtcggcctga aacatgtgct gtgtaccgat
    29881 atctcgcgcg acggcacgct ggcgggatct aacgtctctt tatatgaaga agtgtgtgcc
    29941 agatatccgc aagtggcgtt tcagtcctcc ggcggcattg gcgatattaa cgatgtggcg
    30001 gccctgcgtg gtactggtgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa
    30061 ttcaccgtga aggaggccat cgcatgctgg caaaacgcat aatcccgtgt ctcgacgttc
    30121 gtgatggtca ggtggtgaaa ggcgtacagt ttcgcaacca tgaaatcatt ggcgatatcg
    30181 tgccactggc aaaacgctac gccgaagaag gtgcagatga actggtgttc tacgatatca
    30241 ccgcttccag cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga
    30301 tcgacattcc gttttgtgtg gcgggtggga ttaattctct ggaagatgcc gcgaaaattc
    30361 tttcctttgg cgcggataaa atctccatta actcccctgc gctggcggac ccgacgttaa
    30421 ttactcgcct ggcggatcgc tttggcgtgc agtgtattgt ggtcggcatt gatacctggt
    30481 acgacgccga aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc
    30541 gcgtcactca gtgggaaaca ctcgattggg tgcaggaagt gcaaaaacgc ggtgccggag
    30601 aaatcgtcct caacatgatg aatcaggacg gcgtgcgtaa cggctacgac ctcgaacagc
    30661 tgaaaaaagt gcgtgaagtt tgccacgtcc cgctgattgc ctccggtggc gcaggcacca
    30721 tggaacactt tctcgaagcc ttccgcgatg ccgacgttga cggtgcgctg gcagcttccg
    30781 tattccacaa acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg
    30841 tggagatcag gatatgttaa cagaacaaca acgtcgcgaa ctggactggg aaaaaaccga
    30901 cggtctgatg ccggtgattg tgcaacacgc ggtatctggc gaagtgctaa tgctgggcta
    30961 tatgaacccg gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg
    31021 cactaaacag cgactgtgga ccaaaggcga aacgtcgggt aatttcctca acgtagtaaa
    31081 tattgcgccg gactgcgaca acgacacgtt actggtgctg gcgaatccca tcgggccaac
    31141 ctgccacaaa ggcaccagca gctgcttcgg cgataccgct caccagtggc tgttcctgca
    31201 tcaactggaa caactgctcg ccgaacgtaa atctgccgcc ccggaaacct cctacaccgc
    31261 caaactgtat gccagcggca ctaaacgcat tgcgcagaaa gtgggcgaag aaggcgtgga
    31321 aaccgcactg gcagcaacgg tacatgaccg ctttgaactg accaacgagg cgtctgattt
    31381 gatgtatcac ctgctggtgt tgttgcagga tcaggatctg gatttaacaa cggtgattga
    31441 gaacctgcgt aaacggcatc agtgagttgc gaggtcagcg gatgcgatgt tgttgtcgca
    31501 tccggagcca ttgcccgatg cgcaagctta tcgtgcctac acattatctc aacacacgta
    31561 ggccggataa gatgcgccag catcgcatcc ggcaaaaaaa acgggcatgg tgtcaccacc
    31621 ctgcccgttt ctcttaaatg cacaataata ttatttcgcg ttgtaattac gcagcgcgtt
    31681 acgcccaagc acaatccccg cgccaaccat gccgcccagc agcactgcca gaatcaaggt
    31741 aattgccttt ttcgggctat cgcgacgaat aggtaacgtc ggtttcatta catagcggta
    31801 agcatgaata tcaagtttat caacgtcaag attgtcgata tccagcaggt tttgacgagt
    31861 ctgatagtag tttgatgaga acaccaacgg acgggtcgct tcgtgcttaa tcatcgactc
    31921 cagcgcttcg ctccccaaaa ggaacatagt atcctgggtt acatcctgtg tctgctgaat
    31981 ctgcggcttt gtcacctgcg cctgattcgc atactgcaac gcttcctgaa tctgacggat
    32041 acgcagatct ttctgttctt gagcgaccac ttcctgcgtc ttcagagaat cctgcaagtt
    32101 tttcattcgc agcgcgatgt tgtctttcag gtcttgttcc agttcttcat ttacctggtc
    32161 gtcaacttgc tggatatact tcgccagctg tttctgcgca ccttcaggtg tttcaccgac
    32221 ataagaaacc gctaaaggta atgactgatt tttaacggta ggctcaatgg tcagtttttc
    32281 aggttcttcc tgattatcca gcgtttctgc taatgcagaa aatgtcgtgc tgaaacgacc
    32341 aattaacatc gactggatct cagacgcttt cggcgctgcc gaaccataaa tcacatttat
    32401 ggcattgtta taaccagcga tttgccccac atcaggttga gtaacgatag ctgttgaagt
    32461 ccacttttct ttggcaacgg ccagataccc tatagccagg gcaatagcca caatgacaga
    32521 aatgatgata gtcatcttgc cacgccatag ttggaccagt aaatcaatca aatcaatctg
    32581 ttccgggtca tggttttgcc cagaaacatt attattttct actctcatca ttaccctaac
    32641 ggacaaaata attttaagtc gccatagttt agcgataatc gccatgaatg ctataggata
    32701 aatgataaaa ataatgaatt attacaaagg gaacataaga caatcagggc gaaaatcgcc
    32761 ctgataacag gatgttagtc gctgccaaag agatcgcggg tgtagacttt atctgccaca
    32821 tccttaagct cttctgccat acggttggaa ataatgacgt cggcttgttg tttgaaggtg
    32881 gcgagatcac gttccagacg agagttgaag aatgagtctt ctttcatcac cggctcgtag
    32941 atgatcactt caacgccttt cgccttaata cgtttcataa tcccctgaat ggaagacgcg
    33001 cggaagttat ctgaaccgct cttcataatc agacgataaa tacccaccac ttgcggcttg
    33061 cgtgacaaaa tggcatcggc aataaagtct ttacgcgtgc ggtttgcatc gacaattgcc
    33121 gagatcaggt tattcggcac agactggtag ttcgccagta actgcttggt atctttcggt
    33181 agacaataac caccatagcc aaacgacgga ttgttgtaat ggttgccaat acgcggatcg
    33241 agacaaacac cttcgataat ctgacgagta ttcagaccta aactttctgc atagctatcc
    33301 agttcattaa agtatgctac gcgcatcgcc agataggtat tagcaaacag tttaatcgct
    33361 tctgcttcag tggagtcggt aaacagggtc gggatatttt gcttaatcgc gccttcctgt
    33421 aacaacgcag caaaacgttc tgcgcgttct gaacgctcgc caatgacaat acgtgaagga
    33481 tggagattat cgtaaagagc tttaccttca cggagaaatt ccggagagaa aataaggtta
    33541 tcgataccca gacgttcttt aattgatttg gtgaaaccta ccggaattgt agatttaatc
    33601 accattaccg cgttgggatt aatttctgtc acatcacgga tgaccgcttc gacacttgag
    33661 gtattaaaat aatttgtttt cgggtcataa tcggtaggag tggcaataat aacgtaatcg
    33721 gcatttttat acgcgtcata cttatctgtc gtagcgcgga aattgagatc tttagtcgcc
    33781 agatactctt caatctcctt atcaacaatc ggtgactgct tcttgttaag catgtccact
    33841 ttggcctgaa cgatatccag tgcaaccact tcgtggtttt gcgcaatcag aataccattt
    33901 gaaagaccaa cataacctgt tcctgaaatt gttattttca ttagctctga cttcttccgg
    33961 ttaaacattt agagtggtca ttaatccatc accacaactt catctactgc gggattttta
    34021 acgctgatgt agatgaactg tcaaggcagc gatcctgctg tgcggcgctg tattatatcg
    34081 cttttttaac cataaattat aaaaaaaggc cataacctgc cgctttgtat aataaaaaag
    34141 cccggagggt ttctccgggc cttgcattga ttaattgatt taaatcagat taatccagcc
    34201 attcggtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcg ccgaaatagt
    34261 cacgctgtgc ctggatcagg ttcgcaggca gaacagcggc acggtagctg tcataatagg
    34321 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    34381 cgcgcagcgc ctgctgatag tcatcggcaa tttgcttgaa gtacggagcc agcagcaggt
    34441 tagcgatctg cggattttcg gcatatgcat cggtgatttt ctgcaggaac tgcgcacgga
    34501 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta attcagatcc cagttgtact
    34561 cttcagacgc cgcacgtagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    34621 acagcgcacg acgaactttt tcgatgaact cacctttgtc gccagctggc tgcgcttgcg
    34681 ggccagagag aactttagat gcggcaacac gctgctcttt cagagaagag atataacgtg
    34741 caaacacaga ctcggtaatc agcgacagcg gttctccgag atccagcgcg ctctggctgg
    34801 tccatttacc ggtacctttg ttagccgctt catccaggat cacatcaacc aggtagttac
    34861 cgtcttcatc ttttttggtg aagatatctt tggtgatgtc gatcaggtag ctgcttagtt
    34921 caccgttatt ccactcggta aaggtctgtg ccagttcttc gttagtgagg ttcaagccac
    34981 ctttaagcag agaataggct tcagcaatca gttgcatatc tccgtattca ataccgttgt
    35041 gaaccatctt cacataatgg cctgcaccat cggcaccgat ataggtaaca catggctcac
    35101 cgtcttcagc cactgcggcg attttggtca ggatcggcgc aaccagttca taggcttctt
    35161 tctgcccacc aggcataatg gaaggacctt tcagcgcgcc ttcttcacca ccggagacac
    35221 cggtaccaat gaagttaaag ccttcggcag aaagctcacg gttacgacga atggtgtcct
    35281 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgaggtat ggcttgaggg
    35341 aatcaatagc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    35401 tttccagaga ttcaacaaac tctttcaccg tatagtaagg aaccagtttt ttgcctggat
    35461 tttcggcaat cacttcttcg gtcttttcac gggaacggtt gaaaatagag acggtataac
    35521 cacggctttc gatgttgagc gcaaggttgc gccccatcac tgccataccg acgacgccga
    35581 tctgttgctt tgacattgtt tactcctgtc agggggtggt caccgcgatg tatgcg
//
LOCUS       DADTFG010000031        35589 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.031, whole genome shotgun sequence.
ACCESSION   DADTFG010000031 DADTFG010000000
VERSION     DADTFG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.031"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            210..1176
                     /locus_tag="J1138_001457"
                     /pseudo
     CDS             210..1176
                     /locus_tag="J1138_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1520..2818
                     /gene="kgtP"
                     /locus_tag="J1138_001458"
     CDS             1520..2818
                     /gene="kgtP"
                     /locus_tag="J1138_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBA8453274.1"
                     /translation="MAESTVMADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(2815..3138)
                     /locus_tag="J1138_001459"
     CDS             complement(2815..3138)
                     /locus_tag="J1138_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBA8453275.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(3184..4539)
                     /gene="pssA"
                     /locus_tag="J1138_001460"
     CDS             complement(3184..4539)
                     /gene="pssA"
                     /locus_tag="J1138_001460"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBA8453276.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(4653..7313)
                     /gene="pat"
                     /locus_tag="J1138_001461"
     CDS             complement(4653..7313)
                     /gene="pat"
                     /locus_tag="J1138_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBA8453277.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIARLPFTPDLAVLCTNASRNLAILEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLSDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESALALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQREES"
     gene            complement(7345..8043)
                     /locus_tag="J1138_001462"
     CDS             complement(7345..8043)
                     /locus_tag="J1138_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HBA8453278.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(8112..8531)
                     /gene="trxC"
                     /locus_tag="J1138_001463"
     CDS             complement(8112..8531)
                     /gene="trxC"
                     /locus_tag="J1138_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBA8453279.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLSESL"
     gene            8738..9775
                     /locus_tag="J1138_001464"
     CDS             8738..9775
                     /locus_tag="J1138_001464"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBA8453280.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRCPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(9823..10512)
                     /gene="ung"
                     /locus_tag="J1138_001465"
     CDS             complement(9823..10512)
                     /gene="ung"
                     /locus_tag="J1138_001465"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBA8453281.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            10817..11200
                     /gene="grcA"
                     /locus_tag="J1138_001466"
     CDS             10817..11200
                     /gene="grcA"
                     /locus_tag="J1138_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBA8453282.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(11256..11843)
                     /gene="eamB"
                     /locus_tag="J1138_001467"
     CDS             complement(11256..11843)
                     /gene="eamB"
                     /locus_tag="J1138_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBA8453283.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            11946..12827
                     /locus_tag="J1138_001468"
     CDS             11946..12827
                     /locus_tag="J1138_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8453284.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLALVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(12860..14194)
                     /gene="srmB"
                     /locus_tag="J1138_001469"
     CDS             complement(12860..14194)
                     /gene="srmB"
                     /locus_tag="J1138_001469"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBA8453285.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            14326..15063
                     /gene="trmN"
                     /locus_tag="J1138_001470"
     CDS             14326..15063
                     /gene="trmN"
                     /locus_tag="J1138_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBA8453286.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(15048..16670)
                     /gene="nadB"
                     /locus_tag="J1138_001471"
     CDS             complement(15048..16670)
                     /gene="nadB"
                     /locus_tag="J1138_001471"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBA8453287.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRIANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            16926..17081
                     /gene="rseD"
                     /locus_tag="J1138_001472"
     CDS             16926..17081
                     /gene="rseD"
                     /locus_tag="J1138_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBA8453288.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            17078..17653
                     /gene="rpoE"
                     /locus_tag="J1138_001473"
     CDS             17078..17653
                     /gene="rpoE"
                     /locus_tag="J1138_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBA8453289.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            17686..18336
                     /gene="rseA"
                     /locus_tag="J1138_001474"
     CDS             17686..18336
                     /gene="rseA"
                     /locus_tag="J1138_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBA8453290.1"
                     /translation="MQKEQLSALLDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            18336..19292
                     /gene="rseB"
                     /locus_tag="J1138_001475"
     CDS             18336..19292
                     /gene="rseB"
                     /locus_tag="J1138_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBA8453291.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSSDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            19289..19768
                     /gene="rseC"
                     /locus_tag="J1138_001476"
     CDS             19289..19768
                     /gene="rseC"
                     /locus_tag="J1138_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBA8453292.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGFGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            19966..21765
                     /gene="lepA"
                     /locus_tag="J1138_001477"
     CDS             19966..21765
                     /gene="lepA"
                     /locus_tag="J1138_001477"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBA8453293.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            21781..22755
                     /gene="lepB"
                     /locus_tag="J1138_001478"
     CDS             21781..22755
                     /gene="lepB"
                     /locus_tag="J1138_001478"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBA8453294.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            23022..23702
                     /gene="rnc"
                     /locus_tag="J1138_001479"
     CDS             23022..23702
                     /gene="rnc"
                     /locus_tag="J1138_001479"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBA8453295.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            23699..24604
                     /gene="era"
                     /locus_tag="J1138_001480"
     CDS             23699..24604
                     /gene="era"
                     /locus_tag="J1138_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBA8453296.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            24616..25344
                     /gene="recO"
                     /locus_tag="J1138_001481"
     CDS             24616..25344
                     /gene="recO"
                     /locus_tag="J1138_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBA8453297.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            25356..26087
                     /gene="pdxJ"
                     /locus_tag="J1138_001482"
     CDS             25356..26087
                     /gene="pdxJ"
                     /locus_tag="J1138_001482"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBA8453298.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            26087..26467
                     /locus_tag="J1138_001483"
     CDS             26087..26467
                     /locus_tag="J1138_001483"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBA8453299.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            26888..26968
                     /gene="shoB"
                     /locus_tag="J1138_001484"
     CDS             26888..26968
                     /gene="shoB"
                     /locus_tag="J1138_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBA8453300.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(27162..27422)
                     /gene="yfhL"
                     /locus_tag="J1138_001485"
     CDS             complement(27162..27422)
                     /gene="yfhL"
                     /locus_tag="J1138_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBA8453301.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(27478..28326)
                     /locus_tag="J1138_001486"
     CDS             complement(27478..28326)
                     /locus_tag="J1138_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA8453302.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNTEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            28535..29170
                     /gene="yfhb"
                     /locus_tag="J1138_001487"
     CDS             28535..29170
                     /gene="yfhb"
                     /locus_tag="J1138_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBA8453303.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            29195..29731
                     /gene="tadA"
                     /locus_tag="J1138_001488"
     CDS             29195..29731
                     /gene="tadA"
                     /locus_tag="J1138_001488"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBA8453304.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(29728..31284)
                     /gene="mltF"
                     /locus_tag="J1138_001489"
     CDS             complement(29728..31284)
                     /gene="mltF"
                     /locus_tag="J1138_001489"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBA8453305.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEVAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHTPSLLF
                     SRKGSEEKQN"
     gene            31542..35429
                     /gene="purL"
                     /locus_tag="J1138_001490"
                     /gene_synonym="purI"
     CDS             31542..35429
                     /gene="purL"
                     /locus_tag="J1138_001490"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBA8453306.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITITDAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVIAANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         8613 a         8907 c         9476 g         8593 t
ORIGIN      
        1 ccgcatctta tccgaccttg tattatccct ccagtgcaga gaaaatcggc cagttttctc
       61 tgcctgcagt ccgcatgccg tatccggcct tgggttctaa cctgcatggt agatttatgc
      121 ggcggactgc ctttctcccg aagtgataaa ccggacagta tcatagaccg gttttcccgg
      181 taatccgtat ttgcaaggtt ggtttcacta tggaacatga acttcattat atcggtatcg
      241 acaccgctaa agagaaactg gatgtcgatg tgttgcgtcc tgatggtcgt catcgcacca
      301 aaaaattcgc taacaccact aaagggcacg atgagctggt gagttggctg aaatgtcaca
      361 agattgacca tgcgcatatc tgcatcgaag cgaccggcac ctatatggaa cctgtcgcag
      421 agtgccttta cgatgctggc tacatagtgt cagtcattaa tcctgcactg ggtaaagctt
      481 tcgctcagag tgaatgactg cgtaacaaga ctgataccgt ggatgccgca tgctggcaga
      541 gttctgtcgt cagaagcgcc ctgcagcctg ggaagcgcat cacccgcttg aacgcgcgtt
      601 gcatgccctg gtagtacgcc accaggcgct gacagatatg cacaggcagg aactgaatcg
      661 cactgaaacg gcgcgggaag tccagaggcc gagcattgat gctcaccttc tgtggcttga
      721 agcagagctg aagcgtcttg agaagcagat aaaagacctg acagacgatg atccggatat
      781 gaaacaccgc aggaaactgc tggaaagcat cccgggtatc ggagagaaaa catctgcggt
      841 attgctggct tacatggtct gaaggaccgt tttgcccatg ccagacagtt cgccgctttt
      901 gcgggtctga caccacggcg ttatgaatca ggcagtagtg tgagaggggc aagccggttg
      961 agtaaagccg gacatgtgtc gcttcgcagg ccgttgtata tgcctgcaat ggtagccacc
     1021 agtaagactg agtggggacg ggcgttggca gctaatggca agaaaggaaa ggtgattctc
     1081 ggctcgataa tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcatgccg
     1141 ttcgatgcgt cacggcataa tccggtagct gcgtaaaaat cgcggaaggg atgaaacaac
     1201 agcgcctgac ggcgctgtgt ctggcatgcc tgcaatccgg gagactggac caggaaaaaa
     1261 cttgctggcc gtaacagtat ctacgctccc acataagcca gattcaacag cgaatacggc
     1321 ttccccaaat tgcccacttc catacttcct ccttaccaga aatctatcct taagctcctt
     1381 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcataatgat acgcaacaaa
     1441 tgtataacat tttctttacc aaaaaaataa acaaaagcga ccgacaaaag catcggatta
     1501 cggcaggaga cataatggca tggctgaaag tactgtaatg gcagacagca aactgacaag
     1561 tagtgatact cgtcgccgca tttgggcaat tgttggggcc tcttcaggta atctggtcga
     1621 gtggttcgat ttctatgtct actcgttctg ttcactctac ttcgcccaca tcttcttccc
     1681 ttccgggaac acgacgaccc aactactgca aacagcaggt gtttttgctg caggatttct
     1741 gatgcgccca ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa
     1801 atcgatgctg ttatcggtgt gtatgatgtg tttcggctca ctggttatcg cctgtttgcc
     1861 aggctacgaa acaatcggta cctgggctcc ggcattattg cttctcgcgc gtttattcca
     1921 gggattatcc gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt
     1981 tgaagggcgc aaaggttttt atgcatcatt tcagtatgtg acgttgatcg gcgggcaact
     2041 gctagctcta ctggttgtcg tggttttaca acacaccatg gaagacgctg tactcagaga
     2101 gtggggatgg cgtattcctt tcgcgttagg ggctgtgtta gctgttgtgg cgttgtggtt
     2161 acgtcgtcag ttagatgaaa cttcacaaca agaaacacgc gctttaaaag aagctggatc
     2221 tctgaaagga ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc
     2281 gggctccctt tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc
     2341 gggaatgcat gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct
     2401 tattcaacca cttattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg
     2461 tttcggttct ctggcaacca tttttaccgt tcctattctc tcagcattgc agaacgtttc
     2521 ctcgccttat gccgcttttg gtctggtgat gtgcgccctg ctgatagtga gtttttatac
     2581 ttcaatcagt ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt
     2641 tggtctgtca tatgcggtcg ccaatgctat atttggtggt tcggcggagt acgtagcgtt
     2701 gtcgctgaaa tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt
     2761 ggtggcgttt ctggtttcac taacattgca ccgtaaagga aaagggatgc ggctttagtg
     2821 acgggtcagt tgccagacgg tatagccggt gcttgcaccg gcgatatccc aggcaaaatc
     2881 cttccagctc cagccgctcc cctcagggcg gctatcccaa agctcttttg acgcccccag
     2941 actgacagag aacatcaatc caaacatggc actgcgatcc cggctcatcc cctgatgctg
     3001 tgaatattca tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc
     3061 ctgcccgctc cagttatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca
     3121 gacaaaaaga atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg
     3181 tgattacagg atgcggctaa ttaatcggtc gatgcggata cggcgcagac ggcggatgag
     3241 tttacgaacc ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat
     3301 ggtggtatgc tcgcggatca gctccagttc tttctctcgc tgcggcgcaa gctccagttg
     3361 tggatcgtgg atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt
     3421 attaccggtg atcaacatcc acttatcatc aacccacatc cctttcaggt gataggtgtt
     3481 gtcgtcatct ttccataacc gaactactaa ctggtcagtg ttgacgtaat actgtaaacg
     3541 actcaggaaa cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa
     3601 aggttcatct tccggaatgt agaagtcgtt cgcggtttta tcaccaacaa taatttcgac
     3661 ctttttccct tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa
     3721 gtatggcgta cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt
     3781 gttcaacaga ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc
     3841 ggcatcgccc tggaaatgat aagcggcatc acgcagctcc tggcggaaca ggcgaatgtc
     3901 gttcttgatt tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc
     3961 attcataata ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat
     4021 cagatgataa cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct
     4081 ggcaccgcta taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag
     4141 ggcttcacgg gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg
     4201 cgccatgcgg cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg
     4261 ttgtgcacga tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc
     4321 ctcatacaac gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcaac
     4381 aatgcaaatg cgctgcttcg cgctggctat tttttccagc aacgtctccc ggaagtcggc
     4441 gggagcgtaa aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag
     4501 gtgttgttga tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt
     4561 tcattgaagg gtcctctgtg caatgcagac gacataagcg ggcaataata acaccagtcc
     4621 cgattaagtg gtcaacattt ccagtacctt actcatgatt cctcgcgctg ggcaagactt
     4681 agcgtaagcc caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg
     4741 gccagcgcca ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg
     4801 tgatctcgcg tataggtaat caacttttcc attaagcgtc gacctaagcc taaccctttg
     4861 agatccgagc gaaccagcac ggcaaattcg gcatcgatgt tatcagggtc ggagatcgca
     4921 cgtgtgacgc cgaggatctc ttccgtttga tcaattcgtc gtaccgctac aaatgccatt
     4981 tcccgatcgt agtcgatctg cgtcatgttg gctaaatctt catgggtaaa ttcgttgatc
     5041 tcgctaaagt agcggtaata aagatcttct ttggtgactc gcgaaatgaa ttgctgaagc
     5101 tgtggctcat cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct
     5161 acccattctt ccagctgatg cggataaggg cgcacagcca gccggctctc gttatcacct
     5221 tcaaacggcg cgatatccag cgtgacatcc agcgcggtaa attcactgcc agaagccagc
     5281 aaaggatgaa tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc
     5341 accagaagct ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg cgcacgaatc
     5401 tttttacttt tgatcccctg aataaccaga tagcgggcca agttcatgtt cagcggcggc
     5461 agtgcgacga cggcttgatc ttcaggacgc cactccacac cgccttcacc cagcatgatc
     5521 aacggcccga aaaccggatc gtgctcaacc acaacccgca actcctgagc gccagcacgg
     5581 ttagccatac tttgcaccaa caggccgtgg acccgcgcct gcggccaggc catttttacg
     5641 cgatcgaaaa tagcgtttgc cgcttgctgg acttcattgg ctgtacgtag gtagagcatg
     5701 acgccctgaa cttccgattt atgtggaata tccggcgagc gcaatttcag tgccaccgga
     5761 tagccaatct gttcggcaat atgtaccgct tcggtgctat cactggcaat ccaggtaggg
     5821 agagtattca taccatacgc ttgcaggatg ggctgaactt catgggtatc gagcgacgta
     5881 gccccttcgg caatcgcctg ttgcaacaga agatgcgctt ctgcggtatt ggaagtcaga
     5941 ttgctgggca acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctcgaccata
     6001 tgcataaaag cagtgatggt tccttccgga gtacggtagg tcggcagccc ggcttcgctg
     6061 aataaacgtc gtgcctcttg cgaagagtgt tcgccgcacc agttcgtcag cagagagaca
     6121 tacttgctgc gagggtgatg ctttaccgct tcaattaatg ccagtgcgct ttctgtggcg
     6181 ggagcagcag cactgggcga atgaataacc atcagcgcat caaaatcctg gctgtggagc
     6241 agaatgtcca gcgttttgac ataatgctcg ctgctggcgt catcgcgcag atcgagcggg
     6301 ttagagattg ccacatgccc tggcagtgca tcgctcagtt tctggcaggt ttcttcgctt
     6361 agcgttgcca gcttgccatt gcgtgaccat aaggcatcca gcgccagcgc ggcaggcgca
     6421 gcaccgttgc tgataatcat caaccggtcg ccacgcagcg ggcgcatatg gctaagggtt
     6481 tccaccgccg aaaacagttc gtgggtgtcc tgtacccgca acaaaccggc acgctgaata
     6541 gctgcatccc acgccggatc cattcctgcc gtggtgttca acagacgttg agccgccggg
     6601 ctacgtccgc ttttaatcac caaaatcggt ttattgcgcg aggcactacg ggctgccgaa
     6661 acaaagcgtc gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg
     6721 tcgcgcgcca aatagtcgag caattcatca acgtcgatat ccaggctgtc gccgagcgca
     6781 ataaagtagg aaaagcccat ctcacgctgt tgcgcccagt cgaggatggt gttggagaca
     6841 gcagccgatt gcgaaataaa cgccagcttg ccgcgtttaa tcggcacagg tgaaaagctg
     6901 gcattcagac cttgccaggg agccagtaag cccagactgt ttggtcctag caggcgcatg
     6961 ttatggcgca gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata
     7021 atgcaggttt tacagccttt ctcgccgagc tcttccagaa tagcaagatt acggctggca
     7081 ttggtacata aaaccgcaag gtcgggtgta aagggcaagc gggcaatatc cggccaggcc
     7141 aacacaccca acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct
     7201 cccgccagca ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgatgcgcca
     7261 attaccgcaa tcgattttgg tcgcagtagt gcttccagtc ctcgctgact catatcggtc
     7321 tcccgcagaa gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgcttcc
     7381 cggatgttgc gtttttcctg ccagatagcg tgttttaaag cgggtaaaat gctcgcctaa
     7441 ccctgtcgcc gccctggtat cgccggccat atctaacagt gcgatggcta cctcggcagt
     7501 acaatattgg ccttcagcct gggcttcacg caggcgatag gcagaaagcc gagaaagatc
     7561 gacggaaatg acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc
     7621 tggccaggta ccatcgagca tgataaatag cggaggctta ccggcaggtg gtgtgaagat
     7681 cacttcccgt tgctcatcag catacgaagc gggaaagacc accattggct gatagtccgg
     7741 gttttgcacc agatccagca aatcttgcga gggttcggta cgcgaccatt gaaacgcaac
     7801 ggtatcaggc aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt
     7861 gtcgaacatc agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag
     7921 acataatttc tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag
     7981 aaaaggacgt gttgcgcgcg caatacgctc ggcgcgtaac tggagaacag cgttttcggt
     8041 cataagagag cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg
     8101 ccccggtaag attaaagaga ttcgctcagc cagctgtcga acggcgcttt cggtactgcg
     8161 ccattaagca tgtcgacaac ctgaccattt ttgaaaatca tgatcgtcgg tatactacga
     8221 attccaaagc ggctgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact
     8281 ttaccgctac gctcttgcgc aacatcttca aaaattggcg cgaagttacg gcaggggccg
     8341 caccacggcg cccagaagtc gatcaccaca ggtagatcat ctttcagcaa tttgtcgagc
     8401 gtttcaccgg tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat
     8461 tttgccgcat cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa
     8521 acggtattca taactaacct cgggatgagt aaggagactt atcggcaacg acgccggtat
     8581 gtttctaata tgttacatat tatcggggac attgttccag acaacaatct gttttattca
     8641 atgggtatga tagacacagg ctttggaacg aaatcatggc gaaccgcgcg cacttcgggt
     8701 aatctgcgcg cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa
     8761 aagcggcaag gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca
     8821 caacccgcgt accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg
     8881 ttgccccgcc cgcgatgaca aacagagtca gcaccgtgac cgcaagtggg aagattcgcc
     8941 gtggcgcacg gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg
     9001 catcagtggt aaaagtttta ttgatccgga agttttgcgt cgtcagcgtg cggaagaaac
     9061 ccgcgtctac ggcgaaaacg cctgtcaggc actgttccag agccgtccgg aagcgattgt
     9121 tcgcgcctgg tttatccaga gcgtaacgcc acgttttaaa gaagccttgc gctggatggc
     9181 ggcaaaccgc aaagcgtacc atgtggtgga tgaagcggaa ctgacaaaag cgtcaggcac
     9241 ggaacatcac ggcggcgttt gcttcctgat caaaaagcgt aacggtacaa ccgtgcagca
     9301 gtgggtaagc caggcaggcg cgcaggattg tgtactggcc ctggaaaacg aatctaaccc
     9361 gcataacctg ggcggcatga tgcgtagctg tgcgcacttc ggcgtgaaag gtgttgtggt
     9421 gcaggatgca gcactgctgg agtctggggc ggctatccgt accgcagaag gcggcgcaga
     9481 gcacgttcag ccgattactg gcgacaacat tgttaacgtg ctggatgatt tccgtcaggc
     9541 gggttacacc gtagtgacaa cttccagcga gcagggtaaa ccgctgttca aaaccagtct
     9601 gccagcgaaa atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg
     9661 cgatccgaac gatctgcgcg tgaagattga tggtactggc aacgttgccg ggctgaatat
     9721 ctctgtcgca accggcgttc ttcttggcga atggtggcgt cagaataaag cctgataaat
     9781 cagccgggtg gcaactctgc catccggcat tttcccgcaa atttactcac tctctgccgg
     9841 taatactggc atccagtcaa tcggcgtctc gccacgttgt tccagccact gatttgccag
     9901 cacaaaatgg ttgcagccaa agaatccacg atgcgccgaa agcggcgacg gatgcggtgc
     9961 tttcagtaca tgatggcgtt gtttatctat aatcgcccct ttcttttgcg catgtgatcc
    10021 ccacaacaaa aacaccacgc cttcgcgatg ctgatttatg aggctgatga ctttatcagt
    10081 aaacgtctcc cagccgaggc tggcgtggga atgcgcctga ccagcgcgaa ccgtcaagac
    10141 agtattgagc agcagaacac cctgacgcgc ccagctttca agataaccat gattagggcg
    10201 ggtgaaaccg ggaatagtat tttccagctc tttatacata ttcagcagtg acggtggtgt
    10261 cgcaatgccg gggcgaacgg aaaatgccag accatgcgcc tgtcctggtc cgtgataagg
    10321 atcctgaccg agaatcacca ctttaacgtc acccaactcc gtaaagcgga atgcgttaaa
    10381 gacatctttt tgtggtggat agatagtgac gccggactgc cgctcgctgg cgacggtctg
    10441 aagggtatta agaaaatagg gttgctgctt ctcttcagcc agcacatcat gccaggttaa
    10501 ttcgttagcc atctcgctct cctgcgaatc tttaatccgc ctagcttaac tgcttctttc
    10561 tacagaacaa aatcatgttc attttataga caaaaagaca tgcaaaaaaa tgtaaattgg
    10621 taaaatttca catctgcata agttgatgta aaacaataaa atccatttgt gaccacactt
    10681 gatatatggt ttttgttgat ttaaatcaaa gaatcaaggg tgtttgagga gtatatatac
    10741 actcaagcaa caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt
    10801 gccggggagg catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct
    10861 gctgaactct ttctggttgc tggacagcga aaaaggcgaa gcgcgttgca tcgttgcgaa
    10921 agcaggtttt gcagaagacg aagtggttgc agtaagcaaa ctgggtgaca ttgaataccg
    10981 tgaagttcca gtagaagtga aaccagaagt tcgcgttgaa ggtggtcaac acctgaacgt
    11041 taacgttctg cgtcgtgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca
    11101 gctgaccatc cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca
    11161 gcgcgacgtt atcgctcgta cctttactga aagcctgtaa ggcttaagta gcaagttact
    11221 aagcggaaga gggcgacctc ttccgctttt tttcgttaat agaaaatgcg taccgcgcaa
    11281 tagaccagca acagggcaag cacgatattt aactggcgac catactggcg aaacaatcgc
    11341 tgaaacagat gccccgccag cgcccagcac acattgccaa acgtcccaat catcgccagc
    11401 aaaacgctga cgccaactac ccagcttagc gcctgtgttt gcggcagaac aaacgtcgac
    11461 agtgccgtaa caccgtacaa aatgattttg acgttcacaa actgcaaagc aaagctggcc
    11521 caaaaactga ttggttttgt ctgaagtccg tcttcctttg ttgggctggt ggcgattttc
    11581 cacgccagcc agacaatata tgccgccccc gcccaactca aaaggtgtac cgctgccggg
    11641 tcaatcactg ccagtgaaaa tgaaatgccc gcacacagta acatcacaat caaaaatccc
    11701 agactcatcc ctgccagcac acgggtactt tgacgaaatc catgcgacgt agcagagcta
    11761 agggcgagaa tattgttcgg tcctggcgtc atggcggtaa tcagggtgta agtccaaaaa
    11821 gcacttaaaa gggtcggtgt cacttgttat ctcctctgtt ttggtagaca gtacccgaca
    11881 cggaacattt aaaaaaatga atgtttttac attctaaatt cgatattttc gatagatttg
    11941 gggttatgga tctgcgccgt tttattacac ttaaaaccgt ggtggaagag ggttcctttt
    12001 tgcgagcttc gcaaaaattg tgctgcacac aatcgacggt gacttttcat attcagcagc
    12061 ttgagcagga gttctcggta caattgtttg agaaaattgg tcgacgaatg tgccttaccc
    12121 gcgaaggaaa aaagctgctg ccgcacatct atgaacttac ccgagtgatg gatacgcttc
    12181 gtgaggctgc taaaaaagag tcggatccgg acggggaact acgcgttatt tcaggcgaaa
    12241 cgttgctctc ttatcgaatg ccgcaggtgt tgcagcgttt tcggcaacgc gctccaaaag
    12301 tgcgtttatc gttgcagtcg ctgaactgtt acgtgatccg tgatgcgcta ctgaatgatg
    12361 aggccgatgt cggggttttt tatcgcgtag ggaatgatga tgctctgaac cgacgagagt
    12421 tgggtgaaca accgctggcg ctggtggctt caccgcaaat tgcagatgtc gattttactg
    12481 agccgggaag acataacgcc tgtagcttta ttatcaacga accacaatgt gtcttccggc
    12541 agatatttga gagcacgctg cgccagcggc ggatcacggt ggaaaacacc attgagctta
    12601 taagtatcga aagcatcaag cgttgtgtcg cggcaaatat cggagtcagt tacctgccgc
    12661 gttttgcggt ggtaaaagag ttggagtgcg gagaattaat tgaattgcct tttggcgaac
    12721 agtcgcagac cattacggca atgtgcgctc accatgccgg aaaagcggtt agcccggcga
    12781 tgcacacttt tattcagtgt gttgaagagt gttttttgcc gggataaaaa caatgccggg
    12841 cttaaacccg gcattgagat tactcttctg tcgtttgcgg tggcacgccc gttccgcttg
    12901 gtttacggcg cttaccaata tttttagtgt cgcgatggcg ttttttcacc cgcggcttct
    12961 ctttctcttt agcttttttc ttctcggcac gtttagccaa cactttcttc gatggcttgc
    13021 cggtctgctt ctcgcttggc gcacgcgttt tcggacgtaa ctcatcaatt acgcgagctt
    13081 taattggctc ttcaatataa cggccaactt tgcccagcag cagatggtca tgggcttcca
    13141 ccagcgaaat cgcggtgcct ttacgaccgg cgcgcgcggt acgtccgata cggtgcaaat
    13201 aagtatcgcc actgcgcggc atatcgaagt taaagacgtg gctgacgtca ggaatgtcga
    13261 taccgcgcgc ggcaacatcg gtagcaacca gtacgtttac gcggccttcg gtcaagcgtt
    13321 taatcgcttc gttacgcttg ccttgtacca tctcaccttc gagatagcag ttgttgatgc
    13381 ctgcttcgcg cagccagttt gccagctcat gcacacgctc acgcttacgc acaaacacaa
    13441 ttgagcgggt cgcttccggc tgttttaaca gatgcaccag caacgcggtt ttatgctcaa
    13501 gatcatcggc gcggtaatac cactgatgaa tttttttgcg ctcacgggtg gacggattcg
    13561 cagaaacctc caccggatct tccagcagac gctcggcaaa gtcctgaatc gcatcgcctt
    13621 ccagcgttgc cgaaaagagc agggtctgtt tacgccagcg cgtttcgcca gcaatatgtt
    13681 cgatatcctg ggcgaagccc atatccagca tacggtctgc ttcgtcgagg atcagcgttt
    13741 caaccgcgcg gcaatcgaag ttctcttctt ttatgtattg cagcagacgt ccggtcgtgg
    13801 cgaccacaat gtcctggttt tcgctgaaca cttctgcgtg gttcatatag gccacgccgc
    13861 cggtgatggt ggcaatatcc agatgcgtat gtttcgccag ttcgcgggca tgatcggcca
    13921 cctgcatcgc cagttcgcga gtcggggtaa ggatcaaaat acgcggcgga ccagatttct
    13981 tacgtgggaa atcgagcagg tgctgcaacg ctggcagcag atacgccgcc gttttaccgg
    14041 tgcctgtcgg cgcagacccg agtacatcac ggccatcgag cgcaggcgga atggcggcag
    14101 cctgaatggc ggtcgggcga gtgaaacctt tatcctggag ggcttccagc aggctttcgt
    14161 cgagttcaag ttcggaaaaa gtcgttacag tcatgttcta cctctgtgtg gggcgctgat
    14221 tatagacgtt acggctgcaa tcttcatctg tttgtatgga tatcgcttct cgggtattgt
    14281 tctcacccgg tgctatcgcc gtttttcctg caaggtttta ttttcatgtc acagtctaca
    14341 tccgtgtttc gtcgtaatgg atttactttt aaacagtttt ttgttgctca cgatcgctgt
    14401 gcgatgaaag tgggaacgga tggcattttg ctgggcgcat gggcacctgt ggctggggta
    14461 aaacgttgcc ttgatatcgg cgcgggtagc gggttgttgg cattaatgct ggcgcagcga
    14521 acagatgaca gcgtgatgat tgatgcagtt gaactggaaa gtgaagctgc cgcgcaagcg
    14581 caggaaaata tcaaccagtc cccgtgggca gagcggatta acgtccatac ggcggatatt
    14641 ctgcagtgga tcacacagca gacagtacgc ttcgatttaa tcatcagcaa cccaccttac
    14701 tatcagcagg gagtagagtg cgcgacacct caacgggaac aggctcgcta taccactacg
    14761 cttgatcacc catcgttgct gacttgtgcg gcggagtgca ttactgaaga gggatttttc
    14821 tgcgtggtgc tgccagagca gattggtaat ggttttactg aactggcatt aagtatgggc
    14881 tggcatttac gtttgcggac ggatgtggcg gaaaacgaag cgcgactgcc gcatcgggtg
    14941 ctgctggcat tctccccgca ggcgggagaa tgctttagcg atcgcttagt cattcgtggg
    15001 ccagaccaga actattccga agcgtatacg gcgctgaccc aggcttttta tctgtttatg
    15061 taatgattgc cggcagaaag gatcgatgga ccggaatgcg tgagcagttc cggataatcc
    15121 agcgtgaaat gcaggccacg gctctccttg cgcatcatcg cacagcgaac aatcaactcg
    15181 gcaacctgta ccagattacg cagctccagc aaattatttg agacgcggaa atgggcgtaa
    15241 tattcgtcta tttcttgttg gagcatggtt atccgccgca gggcgcgttc caggcgcttc
    15301 gttgtgcgca caatgccaac gtaatcccac ataaacagac gtagctcgtg ccagttatgc
    15361 tgaattacta cccgttcgtc agggttctca acgcggcttt catcccacgg cggtaacgta
    15421 ctgacgccgt gggcataagg tatgcgtctg ctgatatctt ctgccgccga ccagccatag
    15481 accagacact ccagcaatga attcgaggcc atgcggttag cgccgtgtaa gccggtataa
    15541 ctcacttcgc caatggcata caaaccctcg acgtccgtac gcccatgatc atcaaccatt
    15601 acaccaccgc aggtataatg tgcagcaggc acaatcggca cgggttcttt cgtgagatct
    15661 atccccagcc cgagcaattt ttcataaatc atcgggaaat gctggcgaat aaaatcggcg
    15721 ggcttatggc tgatgtcgag gaacatacaa tccgcgccga ggcgtttcat ctcatggtca
    15781 atggcgcggg cgacaatatc gcgcggagcc agttcgccac gctcatcaaa atcgggcata
    15841 aaacgcgtgc catccgggcg cttgagataa gcgccttcgc cgcgcagtgc ttccgttaac
    15901 aggaaattgc gtgcctgtgg gtgatacagc gcggtagggt ggaactgatt aaattcgaga
    15961 ttggcaatcc ggcagcctgc gcgccacgcc atagcaatgc catcgccaga agaaatatcc
    16021 ggattggtgg tgtactgata aacttttgaa gcaccgccgg ttgccagtac cactgctttt
    16081 gcgtggcagg tttcaaccgt ttctttattt cggttccata cccacgcgcc aacaacccgt
    16141 cgcgtgcccg gcaggccaat tttgtcagaa acaatcagat caaccgcgtt gctgcgctcc
    16201 agcacgcgaa tattcggatg gttctgcgcc ttgcccacca gcgtggtttc tacttctcta
    16261 ccggtggcgt cggcggcatg aagaatacga cggtgactat gtccaccttc acgagtcaga
    16321 tggtagcttt cttcgccatt cggctgaatg tgggtatcaa acaacacccc ctggtcgatt
    16381 agccattgca cacaggatcg tgcattactg gcgacaaatt caactgcatg gcgatcgcaa
    16441 ataccagcac cggcaatcaa tgtgtcttcc acatgcgagt caatgctgtc agtttcatca
    16501 aacacggcgg caataccgcc ctgggcataa aatgttgaac cttccgttac cgggccttta
    16561 cttagaacga tgacctgatg ctggtcagcc aggcgcagcg ccagtgaaag tccggctgcg
    16621 ccgctaccga taatcaacac gtcacatgaa tgttcaggga gagtattcat tttctttgtt
    16681 taatttacta aacacggttt ggtcagcata acatcatgtt gtgcgaataa acacctgcta
    16741 ttttaatatt tgttacagtg actaaacacg ctgcctcagg gcggcgagaa aacgagaagt
    16801 tactggctgg tggaggatta ggtggtgaaa taaaaaggcc gttgggttac tcttcaggca
    16861 gttaaatggg catttctaca cagataatgc gatgttcaga ttctgtagac ttataatgat
    16921 agataatgat ccgtctacag catgacaaac aaaaacagat gcgttacgga actttacaaa
    16981 aacgagacac tctaaccctt tgcttgctca aattgcagct aatggagtgg cgtttcgata
    17041 gcgcgtggaa atttggtttg gggagacttt acctcggatg agcgagcagt taacggacca
    17101 ggtcctggtt gaacgggtcc agaagggaga tcagaaagcc tttaacttac tggtagtgcg
    17161 ctatcagcat aaagtggcga gtctggtttc ccgctatgtg ccgtcgggtg atgttcccga
    17221 tgtggtacaa gaagctttta ttaaagccta tcgtgcgctg gattcgttcc ggggagatag
    17281 cgctttttat acatggctgt atcggattgc tgtaaataca gcgaaaaatt acctggttgc
    17341 tcaggggcgt cgtccacctt ccagtgatgt ggatgccatt gaagctgaaa acttcgaaag
    17401 tggcggcgcg ttgaaagaaa tttcgaaccc tgagaactta atgttgtcag aagaactgag
    17461 acagatagtt ttccgaacta ttgagtccct cccggaagat ttacgcatgg caataacctt
    17521 gcgggagctg gatggcctga gctatgaaga gatagccgct atcatggatt gtccggtagg
    17581 tacggtgcgt tcacgtatct tccgagcgag ggaagctatt gataacaaag ttcaaccgct
    17641 tatcaggcgt tgacgatagc gggatactgg ataagggtat taggcatgca gaaagaacaa
    17701 ctttccgctt tattggatgg cgaaacgctg gatagtgagc tgcttaacga actggctcat
    17761 aacccagaaa tgcagaaaac ctgggaaagc tatcacttga tccgtgactc aatgcggggt
    17821 gatactcccg aggtgctcca tttcgatatc tcttcacgtg tgatggccgc cattgaagaa
    17881 gagccagtac gtcaaccggc gacattgatc ccggaagccc agcctgcgcc gcatcaatgg
    17941 cagaaaatgc cattctggca gaaagtacgt ccgtgggcgg cacagcttac ccaaatgggc
    18001 gtagcagcat gcgtatcgct tgcagttatc gttggcgtcc agcactataa tgggcaatct
    18061 gaaacgtccc agcagcccga aacgccagta tttaatacac tgccgatgat gggtaaagcc
    18121 agcccggtta gtctgggagt accttctgaa gcgaccgcaa ataatggtca acagcagcag
    18181 gtacaggagc agcgtcgtcg cattaatgca atgttgcagg attacgaact gcaacgccga
    18241 ctccactctg aacagcttca gtttgagcag gcacaaactc agcaagccgc tgtacaggtg
    18301 ccaggaattc aaactttagg aacgcaatcg cagtaatgaa gcaactttgg tttgccatgt
    18361 cattagtgac aggtagcctg ttattctctg ctaacgcctc ggccactccc gcgtccgggg
    18421 cgttattaca gcagatgaac ctggccagtc agtcactgaa ttacgagctg tcattcatca
    18481 gcatcaataa acagggtgtt gagtctctgc gttatcgaca tgcacgcctc gataaccgtc
    18541 cgcttgcaca gctgttgcaa atggatggcc cgcgccggga agtggtacag cgcggcaatg
    18601 aaatcagcta ctttgagccg ggccttgaac cgttcacgct taatggcgat tacattgttg
    18661 attctctgcc atcgctcatt tataccgatt tcaaacgcct ttctccttac tacgacttta
    18721 tctccgtggg acgcacgcgt attgctgatc gtctttgcga agtcattcgc gtagtcgccc
    18781 gtgatggcac acgctacagc tacattgtgt ggatggacac cgaatcgaaa ttaccgatgc
    18841 gggttgatct ccttgatcgc gatggtgaaa cgctggaaca atttcgcgtg attgctttta
    18901 acgtcaatca ggatatcagc agcagtatgc agacgctggc gaaggcaaat ttgccgccgt
    18961 tgctttctgt tcctgtaggt gaaaaagcta aattcagctg gacgccaacc tggttgccac
    19021 agggttttag cgaagtttcc agtagtcgac gtccgctacc gacgatggac aacatgccta
    19081 tcgaatcacg tctctattcc gacggattat tcagcttctc ggtaaacgtt aaccgcgcta
    19141 cgccatcgag cagcgatcag atgttgcgca ccggacgcag aaccgtcagt acaagcgtac
    19201 gtgataacgc cgaaatcacc attgtcggtg aactgccgcc gcaaacggcg aaacgcattg
    19261 ccgagaatat taagttcggg gcagcgcaat gatcaaagag tgggctaccg tcgtctcctg
    19321 gcaaaacggg caggcgctgg tcagttgtga tgttaaagcc tcatgcagca gctgcgcttc
    19381 acgcgccggt tgcggtagcc gcgtgttgaa taaacttggc ccgcaaacca cgcataccat
    19441 tgtcgtaccc tgtgatgagc cgttagtgcc ggggcaaaaa gtagaattag gaatcgccga
    19501 aggcagcctg cttagctccg cattactggt ttatatgtcg ccgctggtgg gattattcct
    19561 catcgcttcg ctatttcagc tactctttgc ttctgacgtc gcagcattat gtggtgcgat
    19621 cctcggcggt tttggcgggt tcctgattgc gcgcggctac tcgcgtaagt ttgctgcccg
    19681 ggcagaatgg caaccgatca tcttaagcgt ggccttgccg cctggccttg tgcgatttga
    19741 aacatcttcg gaagacgcga gccagtgatt tcactggctt tgttgcattt tgcataacaa
    19801 atgcgaggac gtttccagat accacaatcc atacggtatg aacatttcct cgcctcaatg
    19861 ttgtagtgta gaatgcggcg tttctattaa tacagacgtt aagctcagaa cagcgacttc
    19921 taaaagcctg gttaaccggg cattaaggca caataatcat actttatgaa gaatatacgt
    19981 aacttttcga tcatagctca cattgaccac ggtaaatcga cgctgtctga ccgtattatc
    20041 cagatctgcg gtggcctgtc tgaccgtgaa atggaggcgc aggttctcga ttccatggat
    20101 cttgagcgtg agcgtggcat taccatcaaa gcgcaaagcg tgacgctgga ctacaaagcg
    20161 tctgacggcg aaacctatca gcttaacttt atcgacaccc cgggccacgt agacttctcc
    20221 tatgaagttt ctcgttcgct ggctgcatgt gaaggtgcat tgctggtggt cgacgccggg
    20281 cagggcgtag aagcgcaaac cctggcaaac tgctacaccg ccatggaaat ggatctcgaa
    20341 gtggtgccgg tactgaacaa gattgacctg ccggcagctg atcctgaacg cgtggcggaa
    20401 gaaattgaag atatcgtcgg catcgacgcc actgacgcgg tgcgctgttc agcgaaaacc
    20461 ggcgttggcg tgcaggacgt tctcgaacgt ctggtgcgcg acattccgcc gccggaaggc
    20521 gatccggaag gcccgttaca ggcactcatt atcgactcct ggttcgacaa ctacctgggc
    20581 gttgtttcac ttatccgtat taaaaacggc accctgcgta agggcgacaa agtgaaagtc
    20641 atgagtaccg ggcagaccta taacgccgac cgtctgggta tttttacgcc aaaacaggtt
    20701 gaccgcactg aactgaaatg tggcgaagta ggctggctgg tatgtgcgat taaagatatc
    20761 cacggcgctc cagtaggcga taccttaacg ctggcgcgta atccggcaga aaaggcgctg
    20821 cctggcttta agaaagtcaa accgcaggta tacgccggtc tgttcccggt aagttccgac
    20881 gactatgaag ccttccgtga cgcgctgggt aaactcagcc tgaacgatgc ctcactgttc
    20941 tatgagccgg aaagctcaag cgcgctgggc tttggtttcc gctgcggctt cctcggtctg
    21001 ctgcacatgg agatcatcca ggaacgtctg gaacgtgaat acgatctgga tctgatcacc
    21061 actgcgccga ccgtagtgta tgaagttgaa accacgtcaa gagaagttat ctacgtcgac
    21121 agcccatcca agctgccagc ggtaaataac atctacgaac tgcgcgagcc gattgcagaa
    21181 tgtcacatgc tgctgccgca ggcatatctc ggcaacgtta ttacgctgtg cgtagaaaaa
    21241 cgcggcgtgc agaccaatat ggtttaccac ggtaatcagg tggcgctgac gtacgagatc
    21301 ccgatggcgg aagtggtgct cgatttcttc gatcgcctga aatctacctc gcgtggttat
    21361 gcgtctctgg attacaactt caaacgcttc caggcgtccg acatggtacg tgtagacgta
    21421 ttaatcaacg gtgaacgtgt tgatgcgctg gcgttgatca cccaccgtga taattcgcaa
    21481 aaccgcggtc gcgagctggt ggagaagatg aaagatctaa tcccacgcca gcagtttgat
    21541 atcgctattc aggcagcgat tggtacgcac atcattgcgc gctccaccgt gaaacagctg
    21601 cgtaaaaacg tactggctaa atgttatggc ggcgatatca gccgtaagaa aaagctgctg
    21661 cagaagcaga aagaaggtaa gaaacgcatg aagcagatcg gtaacgtcga gctgccgcag
    21721 gaagcgttcc tcgccattct gcacgtcggc aaagacaaca aataaccctt aggagttggc
    21781 atggcgaata tgtttgccct gattctggtg attgccacac tagtgacggg cattttatgg
    21841 tgcgtggata aattcttttt cgcacctaaa cggcgggaac gtcaggcagc ggcgcaggcg
    21901 gctgccggtg actcactgga taaagcaaca ctgaaaaagg ttgcgccgaa gcctggctgg
    21961 ctggaaactg gtgcttctgt ttttccggtg ctggctatcg tattgattgt gcgttcgttt
    22021 atttatgaac cgttccagat cccgtcaggt tcgatgatgc cgactctgtt aatcggtgat
    22081 tttattctgg tagagaagtt tgcttatggc attaaagatc ctatttacca gaaaacactg
    22141 atcgaaaccg gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc ggaagatcca
    22201 aagcttgatt acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac ttatgatccg
    22261 gtctcaaaag agctgacgat tcaaccggga tgcagttccg gtcaggcatg tgaaaacgcg
    22321 ctgccggtca cctactcaaa cgtggaaccg agcgatttcg ttcagacctt ctcacgccgt
    22381 aatggtgggg aagcgaccag cggattcttt gaagtgccga aaaacgaaac caaagaaaat
    22441 ggaattcgtc tttccgagcg taaagagaca ctgggtgatg tgacgcaccg cattctgaca
    22501 gtgccgattg cgcaggacca ggtggggatg tattaccagc agccagggca acaactggca
    22561 acctggattg taccgccggg gcaatacttc atgatgggcg ataaccgcga caacagcgcg
    22621 gacagccgtt actggggctt tgtgccggaa gcgaatctgg tcggtcgggc aacggctatc
    22681 tggatgagtt tcgataagca agaaggcgaa tggccgacag gtgtgcgctt aagtcgcatt
    22741 ggcggcatcc attaatagcc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    22801 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    22861 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    22921 ccccatatac cgctaaactg cagcgaagca gttagcagag ctatgtatat caggtctgtt
    22981 tcgtgtgctg aattgttgac gcatttattt attggtatcg catgaacccc atcgtaatta
    23041 atcggcttca acggaagctg ggctacactt ttaatcatca ggaactgttg cagcaggcat
    23101 taacccatcg tagcgccagc agcaaacata acgagcgttt agaattttta ggcgactcta
    23161 ttctgagcta cgttatcgcc aacgcgcttt atcaccgttt ccctcgtgtc gatgaaggcg
    23221 atatgagccg gatgcgcgcc acgctggtcc gtggcaatac gctggcagaa ctggcgcgcg
    23281 aatttgagtt aggcgagtgc ttacgtttag ggccaggtga acttaaaagc ggtggatttc
    23341 gtcgtgagtc aattctcgcc gacaccgtcg aagcattaat tggtggcgta ttcctcgaca
    23401 gtgatattca aaccgtcgag aaattaatcc tcaactggta tcaaacccgt ctggacgaaa
    23461 ttagcccagg agataaacaa aaagatccga aaacgcgctt gcaagaatat ttgcagggtc
    23521 gccatctgcc gctgccgact tatctggtag tccaggtacg tggcgaagcg cacgatcagg
    23581 aatttactat ccactgccag gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa
    23641 gccgtcgtaa ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg gagctggaat
    23701 gagcatcgat aaaagttact gcggatttat tgctatcgtc ggacgtccga acgttggcaa
    23761 atccacattg ttgaacaaac tgctggggca gaagatctcc attacctccc gcaaggcgca
    23821 aacaactcgt caccgcattg tggggatcca tactgaaggc gcgtatcagg cgatctacgt
    23881 tgatacccca ggcctgcata tggaagaaaa acgcgctatt aaccgtctga tgaacaaagc
    23941 ggcgagcagc tccattggcg acgttgagct ggtgattttt gtcgtagaag gcacccgctg
    24001 gacgccggac gatgaaatgg tgctcaacaa gctgcgtgat ggcaaagcgc cggtaatcct
    24061 cgcggtgaac aaagtggaca acgtgcagga aaaagccgat ctgctgccac acttacagtt
    24121 cctggcaagc cagatgaact tcctcgatat cgtgccgatc tctgccgaaa ccgggctgaa
    24181 tgtcgacact atcgcggcaa tcgtgcgtaa gcatctgcct gaagcgaccc atcacttccc
    24241 ggaagattac atcaccgatc gctcacagcg ttttatggcg tctgaaatta tccgcgaaaa
    24301 actgatgcgt tttctcggcg ctgaactgcc gtactccgtg accgtggaga tcgaacgttt
    24361 cgtctctaac gaacgcggcg gttatgacat caacggtttg attctcgttg agcgtgaagg
    24421 gcagaagaag atggtcattg gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc
    24481 gcgtaaagac atgcaggaaa tgttcgaagc gcctgttcac cttgagctgt gggtaaaagt
    24541 gaaatccggt tgggccgacg acgaacgcgc actgcgcagt ctcggttatg ttgacgatct
    24601 ttaagagtaa ctccgatgga aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg
    24661 agcgaaacca gcctgatgct ggacgtcttc acggaggaat cgggtcgcgt gcgtctggtt
    24721 gccaaaggcg cacgctctaa acgctctact ctgaaagggg cattacagcc tttcacccct
    24781 ctcttgctac gttttggcgg gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc
    24841 tcgctggcgc tgccattaag cggtatcacg ctttacagcg gtctgtacat caacgaactt
    24901 ctctcccgcg tactggaata cgagacgcgc ttctctgaac tctttttcga ttacttgcac
    24961 tgcattcagt ctcttgccgg ggtcactggt acgccagaac ccgcgttgcg ccgctttgaa
    25021 ctggcactgc tcgggcatct gggctatggc gtcaatttta cccattgtgc gggtagcggc
    25081 gagccggtag atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc
    25141 gtcgttatcg acaataaaac gttcaccgga aggcagttaa aagcgttaaa cgcgcgggaa
    25201 tttcctgacg cagacacact gcgcgccgcg aaacgcttta cccgcatggc gcttaagccg
    25261 tatcttggcg gtaaaccatt aaagagcagg gaactgttcc ggcagtttat gcctaagcga
    25321 acggtgaaaa cacattatga atgatgagga ttgtcatggc tgaattactg ttaggcgtca
    25381 acattgacca tattgcaacg ctgcgtaacg cgcgcggtac tgcttacccg gatccggtgc
    25441 aggcggcgtt tattgctgag caggcgggag cggacggcat taccgtgcat ttacgtgaag
    25501 atcgccgcca cattaccgac cgcgatgtgt gcatcctgcg ccagacgctg gatacccgca
    25561 tgaatctgga gatggcggtg accgaagaga tgctggcgat cgccgttgag acgaagccac
    25621 atttttgctg tctggtaccg gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg
    25681 tcgcagggca gcgtgaaaaa atgcgcgatg cctgcaaacg tctggcagat gccgggatcc
    25741 aggtttctct gtttatcgac gccgatgaag agcagatcaa agctgcggca gaggttggcg
    25801 caccgtttat cgagatccac accggttgct atgctgatgc caaaactgac gccgaacagg
    25861 cgcaagagtt ggcgcgtatc gccaaagccg cgacctttgc cactagtctc ggtctgaaag
    25921 ttaacgccgg acacggtctg acctatcaca acgtgaaagc cattgccgcc atccctgaga
    25981 tgcatgaact gaatatcggt catgccatta ttggtcgtgc agtgatgacc ggactgaaag
    26041 atgcggtggc agaaatgaaa cgtctgatgc tggaagcgcg tggctaatgg caatattagg
    26101 tttaggtacg gatattgtag agatcgctcg catagaagcg gtgatcgccc gatccggtga
    26161 gcgcctggca cgccgcgtat taagcgataa cgaatgggaa atctggaaaa cgcaccacca
    26221 gccagtgcgt tttctggcga agcgttttgc tgtgaaagaa gcggcggcaa aagcatttgg
    26281 cacagggatc cgcaatggtc tggcgtttaa tcagtttgaa gtattcaatg atgagctcgg
    26341 caaaccacga ctacggctat ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt
    26401 aaatatgcat gtaacgcttg ccgatgagcg ccactatgct tgtgccacgg taattattga
    26461 aagttaaaat aataccgttc tctttataaa gtaaaacccc cgaactggta aggaacgggg
    26521 gttttacttt ttaactttaa ttttgtgcag cacacgattc caacgaaaat gattatggat
    26581 caatttcgtt gacctgtaca agcaacttgc aatctctgac ataaaaaacg cgagccttta
    26641 cgaagaaatc gacattgatg aaaagattaa cgtgaccgct ttttgataag tacattaaaa
    26701 tcggtttcgt tactggcaat tttgctgtgc tttacaccat gccactgaat tcccccattg
    26761 aaacgagtgg tgtcgtcaaa gctctggtgt ggagtgcagc actcgattcc aataactcgc
    26821 acgttcagtt ttggggagat gtaagggcta atctgaacgg ctgcattcct tgtttaagga
    26881 aaaacgaatg actgattgcc gatacctgat taaacgggtc atcaaaatca tcattgctgt
    26941 tttacagctg ctccttttgt tcttataaca caaggaaacg tacttaaggt gcgtccggtg
    27001 aaccagtcgg acgcgccttt aataactata aataagtgtc tgggcagata ctatataaat
    27061 taacttagtg aatgattatg ctaatgtcat catttaaata aatataatag cgttaagatt
    27121 ttcctataaa ataattaaca ctctacttcc agagcagaat attaaatttt atccgcgtgg
    27181 tgcatcagca caaatttatc ccacaactgt tcttccgtct cgacatgttg cggatctttc
    27241 acaatagtat tggggatcgg acacaccttc tggcaggttg gtgtctcgta gtgccctacg
    27301 cattcggtac acttatcgct gttaatctcg tagatatgtt cacccatcga aatcgcctca
    27361 ttcgggcatt cgggttcaca catatcacaa ttgatgcatt ttttagtgat taacagcgcc
    27421 ataaggaaac tctcaaaaca acacaaaccg ggcgggcatt atacgcgcat tctttactca
    27481 gaccagtttt ttcaccagcg cttcgctatg acgaatacgt tctggtgcca gttccagatc
    27541 ctgctgaatc agcgcaatga acagcaaatc cgttaacatt ccctgcgcgt gacaagcaga
    27601 gattgaagca ctgtttgtcg cttgttcttc ggcaatggta tacaggcaat gagaagcacg
    27661 ctgttgcagg gcattgggag taaagccggt aatagccagc acttttccgc ccactcgcag
    27721 catctcatct gccgccaggt ttaactcgcg tcgtacaccg gtgtaggaaa tggctaataa
    27781 cagatcgtca ggggacgacg cctgtactgt agcgagtagc gcatgcatat cgcgcactgc
    27841 ggcagcattg aagccaatct tcatcagctt ccaggcaaag ttttgcgcca ccagacccga
    27901 agcgccaata ccggtcagaa ttatccgccg cgcagagcgc aacattgtta cgcattcatg
    27961 cagtttctct tcagtattaa cgtttagcgt tgcgtacata gcggcagtat tttctttaat
    28021 cagtttttcg ccgaccagcc gtaacggatc atcaccgcgg atttggttat gaatgggcac
    28081 ggagggagat tccggctggc ttgccagcgc ttcactcaac gccaacttta gtgccggaaa
    28141 acctttatag ccgagttttt gcgcgaactt cacgacgctg gactgactga ctccggcttc
    28201 gttggccagt tgctgagagc ttaaatggcg cgccgtatca ggttgtagca gcagataatc
    28261 cgccagtttt ttatcactct gggcaagccc ctggtaacgc tgacggatac gaagtaagcc
    28321 gttcatatct ttcctcgcga tgaaataatg gcgacaacag gcaatatgag acacacgcga
    28381 gtgaatttta tattccattg ccgctaattg ccatgaatta aaaattccag ttagccatga
    28441 tgcggtcgat tgttgccgga aaagtgatga tctatctgtt aggctatgta gtagcgaaaa
    28501 ttaatcagga tgtttcagtc cagaggagta tggtttggca actcacgagc gtcgtgtggt
    28561 gttttttgac ttagatggaa cattgcatca gcaggatatg ttcggcagtt ttttgcgtta
    28621 tttactgcgt cgccaaccgc tgaatgcgtt acttgttctg ccgttgttgc cgatcatagc
    28681 cattgcgtta ttgataaaag gtcgtgcggc acgctggccg atgagtctgc ttctgtgggg
    28741 atgcactttt ggtcacaatg aagcgcgttt gcaggcgttg caggccgatt tcgtacgctg
    28801 gtttcgtgac aatgttaccg cctttccgct ggttcaggag cgattaacca cctacctgtt
    28861 aagttccgat gctgatatct ggttgattac cggctctccg cagccgctgg ttgaagcggt
    28921 ttatttcgat acgccctggc tgccgcgggt taatcttatc gccagtcaga ttcagcgtgg
    28981 ctatggtggt tgggtattga cgatgcgttg tctgggacat gaaaaggtcg cgcaactgga
    29041 gcgcaaaatc ggcacgccgc tgcggctgta cagtggctat agcgacagta atcaggacaa
    29101 cccgctgctt tatttctgtc agcatcgttg gcgagtaact ccgcgcggtg aactccagca
    29161 actggaatag agtaaagcat agcgtccgtg tataatgcgc cgcgctttta taaccggagt
    29221 tttctttttg tctgaagtcg aatttagcca cgaatactgg atgcgtcatg caatgacgct
    29281 ggcgaagcgt gcctgggatg agcgggaagt accggtcggc gcggtattag tgcataacaa
    29341 tcgtgttatt ggcgaaggct ggaaccgtcc gattggtcga catgatccca ccgcacatgc
    29401 agaaatcatg gccctacggc agggcggtct ggtgatgcaa aattatcgtc tgatcgacgc
    29461 cacgttgtat gtcacgcttg aaccctgtgt aatgtgtgcc ggagcgatga tccacagtcg
    29521 cattggtcgc gtggtctttg gtgcgcgtga cgcgaaaact ggcgctgcgg gatctttaat
    29581 ggatgtgcta catcatccgg gtatgaatca ccgggtggaa attacggaag gaatactggc
    29641 ggatgagtgc gcggcgttgc tcagtgactt ctttcgcatg cgccgccagg aaattaaagc
    29701 gcagaaaaaa gcccaatcct cgacagatta attttgtttc tcttcactcc cttttctgga
    29761 aaacagcaga gagggagtgt gggtcaaata gtttgatgac gactgggaaa gaaacgagag
    29821 aaaaggaaat ttctctttgc ccagctccgt aggcgatacc gccggataat cctgcgccag
    29881 ttgcatcgcc acttctgtag cctgcttctc tttctcctgc agataaccca ccaggctaat
    29941 ctgatactta cgaatatttt cgacataagc gtaggcttca tgcccacgag cgtagccgta
    30001 agtcagcttg ctgtaatagg gtttctggct aagtaaaggc agacgctgtt ttacgtcagc
    30061 ccaactgtca ggattacctt tggtttttgc cgtcagggcg cgggcatcca gcatatgcgc
    30121 atagcccata ttgtacgcgg cgagggcaaa ccagatccgt tcgttctccg gcacactttc
    30181 cggcacttta ctcatcatat cctgcaaata acgcacgccg ccgctgatgc tctgttcggc
    30241 atcggtacga tccgtaatgc cgaggctttg cgcggtgttt ttggttaaca tcatcatgcc
    30301 gcgcacaccc gtcggtgaag tggcctgagc atcccagtgc gattcctgat aagcaatagc
    30361 ggccagcaaa cgccagtcaa tttcttcggc gtatttctca aacaggggct ttaactgcgg
    30421 cagtaccgca tcgacggcgc gtaaaaatgt gcgcgtatcg acgtaatcaa aatcatcgcc
    30481 atgccccagg tatttctctt caatgcgtgc cagtgtaccg tcttcattca tttcgttgaa
    30541 gaagtcgagc agggcggcgg aaagggtatt atcgccatct aacgggctaa accaggtcac
    30601 cggttgttca tcggtgatat cgagcgctac ggcgagttcc gggtgaacgc gctgaaacag
    30661 gctgatggcg acagaatcag caatggtgta atcgagtttt ccttcgataa catcttccat
    30721 taattccgca gagccttttt tgtcgtctac cttccagctt aattccggga attttgtgtc
    30781 tttcagggtc tggagatcgt taaccaccac gtgacccggt gcaacggtga gttgctccgc
    30841 cgtcaggttg cccagcgtac gtgggcgata ctgaccgact ttataaacca gttgttgtga
    30901 cacggaataa taggtaggac caggctgata attttttacc cgctcactgt tatagaccag
    30961 tcctgccgcc agcaggtcgg cgttaccatt atcgaggtcg tcaaacagct ggctgatatt
    31021 ctgccgcacg gtcactttca gttttacacc gaggtaatcg gcaaactgtt tcgccagttc
    31081 gtaatccagg ccaaaaggtt tcccgttgat ttcgttataa gtcaggggag tatgaatggt
    31141 gctcacacgc aactctcccc gcgcttgaat ggcggcgata cggttgtcgg ctttaccaaa
    31201 ccagggaatg gatggccaga gagcgaccgc gagcagcagt gccagaatgc cgatgaacag
    31261 ataattaatc tttaattttt tcaattagtt aattctctgt gtcgtgcgcg tcccagcttg
    31321 aaaaaacgta ataatagtga aaggtttact cataaatgag cggcattttg cgtaaacctg
    31381 cgccagttgg caacttatta cagccattgg cggcacgcgt tgctaattca cgatggtgat
    31441 tttatttcca cgcaaacggt ttcgtcagcg catcagattc tttataatga cgcccgtttc
    31501 ccccccttgg gtacaccgaa agcttagaag acgagagact tatgatggaa attctgcgtg
    31561 gttcgcctgc attgtcggca ttccgaatca acaaactgct ggcacgtttt caggctgcca
    31621 ggctcccggt tcacaatatt tacgccgagt atgtccattt tgctgacctc aatgcgccgt
    31681 taaacgatga tgagcacgca caacttgaac gcctgctgaa atatggcccg gcactcgcca
    31741 gccacgcccc gcaaggcaaa ctcctgctgg tgaccccgcg tcctggcacc atctctccct
    31801 ggtcttcgaa agcgaccgat atcgcccata attgcggtct acaacaggta aaccgccttg
    31861 agcgcggcgt tgcttactat gtagaagccg gtacgctgac caatgaacaa tggcagcagg
    31921 tgactgctga actgcatgac cgcatgatgg aaacggtctt ttttgcttta gatgatgcag
    31981 agcagttgtt tgcacaccat caaccgactc cggtcaccag cgtagatttg ctggggcagg
    32041 gccgtcaggc gctaatcgac gctaacctgc gtcttggcct ggcactggcg gaagatgaaa
    32101 ttgactatct gcaggatgcg ttcacaaagc tgggtcgtaa cccgaacgac atcgagctgt
    32161 atatgttcgc gcaggcgaac tccgagcact gccgtcacaa aatttttaac gccgactgga
    32221 ttatcgacgg tgaacagcag ccgaaatcgc tgttcaagat gatcaaaaac accttcgaaa
    32281 ccacgccaga ccacgttctc tctgcttata aagataacgc cgccgtaatg gaaggttctg
    32341 aagtgggtcg ctactttgct gaccacgaaa cgggccgcta cgatttccat caggaaccgg
    32401 cgcatattct gatgaaagtt gaaacccaca accacccaac ggcgatttct ccgtggccgg
    32461 gcgcggcgac cggttccggc ggtgaaatcc gcgatgaagg tgccaccggc cgtggcgcaa
    32521 agccgaaagc tggtctggtt ggtttctccg tatccaacct gcgaattcct ggcttcgaac
    32581 agccgtggga agaagatttc ggtaaacctg agcgcattgt taccgcgctg gacatcatga
    32641 ccgaaggccc gctgggcggt gcggcgttta acaacgaatt tggtcgtccg gcactgaacg
    32701 gctacttccg tacttatgaa gaaaaagtga acagccacaa tggcgaagag ctgcgcggtt
    32761 atcacaaacc gatcatgctg gcgggtggga tcggcaacat tcgcgccgat catgtacaaa
    32821 aaggcgagat caacgttggt gcgaagctgg tcgttctcgg cggcccggca atgaacatcg
    32881 gtcttggcgg cggcgcggcg tcttccatgg cgtctggtca gtctgatgcc gacctcgact
    32941 ttgcttccgt acagcgcgac aacccggaga tggagcgtcg ctgccaggaa gtgatcgacc
    33001 gttgctggca gcttggtgat gccaacccaa tcctgtttat ccacgacgtt ggcgcaggcg
    33061 gtctttctaa cgccatgccg gaactggtga gcgacggcgg gcgcggcggt aaatttgaac
    33121 tgcgcgatat cctgagcgac gaaccgggca tgagcccgct ggaaatctgg tgtaacgaat
    33181 cccaggaacg ctacgtgctg gcggttgctg ccgatcagtt accgctgttt gacgaactgt
    33241 gtaagcgtga gcgcgcgcca tacgcggtga ttggtgaagc gaccgaagaa ctgcatcttt
    33301 ctttgcacga tcgtcatttt gataatcagc cgattgatct accgctggac gtactgcttg
    33361 gcaaaacgcc gaagatgacc cgcgatgtac aaacgctgaa agcgaaaggc gacgcgctgg
    33421 cccgtgaagg gatcaccatc actgacgcgg tgaaacgcgt gctgcatctg ccgaccgtgg
    33481 cggagaaaac tttcctggtg accatcggtg accgcagcgt aaccggcatg gtggcgcgcg
    33541 atcagatggt ggggccgtgg caggtgccgg tcgctaactg cgcggtcact accgccagcc
    33601 tcgacagcta ctatggcgaa gcgatggcaa ttggcgaacg tgcgccagtt gcgctgctgg
    33661 atttcgccgc ttctgcccgt ctggcagtcg gtgaagcgct aaccaacatc gccgcaacac
    33721 aaattggcga tatcaaacgc attaaacttt ccgccaactg gatggcggcg gcaggccacc
    33781 ctggtgaaga tgctggcctg tatgaagccg ttaaagctgt gggtgaagaa ctttgtccgg
    33841 cgctgggcct gacgatcccg gtgggtaaag actctatgtc gatgaaaacc cgctggcagg
    33901 aaggtaacga agagcgcgaa atgacgtcgc cgttgtcgct ggtgatttcc gcatttgccc
    33961 gcgtggaaga tgtacgtcac accattacgc cgcagctttc taccgaagat aacgcactgt
    34021 tgctgattga tttgggcaaa ggcaataacg cgctgggcgc aacggcactg gcgcaggttt
    34081 atcgtcagct tggcgacaaa ccggcagatg tacgcgatgt tgcccaactg aaaggctttt
    34141 atgacgcgat tcaggcgctg gttgcacagc gtaagctgct ggcgtatcac gaccgctctg
    34201 atggcggcct gctggtaacg ctggcggaaa tggcctttgc tggtcattgt ggcattgacg
    34261 cagatatcgc cactctgggt gacgatcgcc tggcggcgtt gtttaacgaa gaactgggtg
    34321 cggtgattca ggtgcgagcc gctgaccgtg aagccgttga agcggtactg gcacagcatg
    34381 ggcttgctga ttgcgtccat tatgttgggc aggcggtttc cggtgaccgt tttgtgattg
    34441 ccgccaacgg gcagactgta tttagcgaaa gccgcaccac gttgcgtgtc tggtgggcag
    34501 aaactacctg gcagatgcag cgtctgcgtg acaacccgga gtgtgccgat caggagcacc
    34561 aggcgaaatc aaacgacgcc gatccgggcc tgaatgtgaa actgtcgttt gatatcaacg
    34621 aagatgtggc agcaccgttt attgccactg gcgcgcgccc gaaagttgcc gtgctgcgtg
    34681 agcagggcgt gaactcgcat gttgaaatgg cggcagcttt ccaccgtgca ggcttcgatg
    34741 ctatcgacgt gcatatgagc gatctgctgg ctggacgcac gggcctggag ggtttccacg
    34801 ccctggtagc gtgcggtggt ttctcctacg gtgatgtgct gggcgctggt gaaggttggg
    34861 cgaagtctat cctgttcaat gaccgtgtac gcgatgagtt tgcaaccttc ttccatcgtc
    34921 cgcaaacgct ggcgctgggg gtatgtaacg gttgccagat gatgtctaat ctgcgtgaac
    34981 tgatcccggg tagcgagttg tggccacgtt ttgtgcgtaa cacctccgat cgctttgaag
    35041 cgcgtttcag tctggtagaa gtaacccaaa gcccgtcgct gctgttgcag gggatggtgg
    35101 gctcgcagat gccgattgct gtctctcacg gtgaagggcg cgttgaagtg cgcgatgcgg
    35161 cgcatctggc ggcactggaa agcaaagggc tggtggcact gcgctatgtc gataacttcg
    35221 gcaaagtcac tgaaacctac ccggctaacc cgaacggttc accgaacggt attacggcgg
    35281 tcacgactga aagtggtcga gtcaccatta tgatgccgca cccggaacga gttttccgta
    35341 ctgtcagcaa ctcctggcat ccggaaaact ggggcgagga tggcccatgg atgcgcattt
    35401 tccgcaatgc acgtaagcag ttggggtaag tcgtcaaccc attggtattg cagcccggat
    35461 gaggcattca cgccgcatcc ggcattaagc accaaccgct attatgagct cccaacttcg
    35521 ggagcttttt tgtgtctgta aatcacgaca atgggtggtt tgccgtgtcg ctttttggtg
    35581 acacttaac
//
LOCUS       DADTFG010000032        35282 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.032, whole genome shotgun sequence.
ACCESSION   DADTFG010000032 DADTFG010000000
VERSION     DADTFG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.032"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..447)
                     /locus_tag="J1138_001491"
     CDS             complement(<1..447)
                     /locus_tag="J1138_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA8453307.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDD"
     gene            complement(705..1958)
                     /locus_tag="J1138_001492"
     CDS             complement(705..1958)
                     /locus_tag="J1138_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HBA8453308.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2190..3521
                     /gene="argA"
                     /locus_tag="J1138_001493"
     CDS             2190..3521
                     /gene="argA"
                     /locus_tag="J1138_001493"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBA8453309.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAQTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3583..5409)
                     /gene="recD"
                     /locus_tag="J1138_001494"
     CDS             complement(3583..5409)
                     /gene="recD"
                     /locus_tag="J1138_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBA8453310.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(5409..8951)
                     /gene="recB"
                     /locus_tag="J1138_001495"
     CDS             complement(5409..8951)
                     /gene="recB"
                     /locus_tag="J1138_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBA8453311.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYARLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLTREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCNDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITTVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFASMTLEEA"
     gene            complement(8944..11832)
                     /gene="ptrA"
                     /locus_tag="J1138_001496"
     CDS             complement(8944..11832)
                     /gene="ptrA"
                     /locus_tag="J1138_001496"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBA8453312.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAIFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRTMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12008..15376)
                     /gene="recC"
                     /locus_tag="J1138_001497"
     CDS             complement(12008..15376)
                     /gene="recC"
                     /locus_tag="J1138_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBA8453313.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLSS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCHHTRMPFDPQNYQPGN
                     LQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15389..15712)
                     /locus_tag="J1138_001498"
     CDS             complement(15389..15712)
                     /locus_tag="J1138_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBA8453314.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15697..16104)
                     /locus_tag="J1138_001499"
     CDS             complement(15697..16104)
                     /locus_tag="J1138_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBA8453315.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16101..16664)
                     /locus_tag="J1138_001500"
     CDS             complement(16101..16664)
                     /locus_tag="J1138_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBA8453316.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGNCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16655..17125)
                     /locus_tag="J1138_001501"
     CDS             complement(16655..17125)
                     /locus_tag="J1138_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBA8453317.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17310..18104)
                     /gene="thyA"
                     /locus_tag="J1138_001502"
     CDS             complement(17310..18104)
                     /gene="thyA"
                     /locus_tag="J1138_001502"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBA8453318.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18111..18986)
                     /gene="lgt"
                     /locus_tag="J1138_001503"
     CDS             complement(18111..18986)
                     /gene="lgt"
                     /locus_tag="J1138_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBA8453319.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19137..21383)
                     /gene="ptsP"
                     /locus_tag="J1138_001504"
     CDS             complement(19137..21383)
                     /gene="ptsP"
                     /locus_tag="J1138_001504"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA8453320.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21396..21926)
                     /gene="rppH"
                     /locus_tag="J1138_001505"
     CDS             complement(21396..21926)
                     /gene="rppH"
                     /locus_tag="J1138_001505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBA8453321.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22611..23300
                     /gene="mutH"
                     /locus_tag="J1138_001506"
     CDS             22611..23300
                     /gene="mutH"
                     /locus_tag="J1138_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBA8453322.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23369..24082
                     /locus_tag="J1138_001507"
     CDS             23369..24082
                     /locus_tag="J1138_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA8453323.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24220..24438
                     /gene="ygdR"
                     /locus_tag="J1138_001508"
     CDS             24220..24438
                     /gene="ygdR"
                     /locus_tag="J1138_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBA8453324.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24546..25586
                     /locus_tag="J1138_001509"
     CDS             24546..25586
                     /locus_tag="J1138_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBA8453325.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25618..26811)
                     /gene="lplT"
                     /locus_tag="J1138_001510"
     CDS             complement(25618..26811)
                     /gene="lplT"
                     /locus_tag="J1138_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBA8453326.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHILVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26804..28963)
                     /gene="aas"
                     /locus_tag="J1138_001511"
     CDS             complement(26804..28963)
                     /gene="aas"
                     /locus_tag="J1138_001511"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBA8453327.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLEGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAAISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEAALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAKVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29549..30580
                     /gene="galR"
                     /locus_tag="J1138_001512"
     CDS             29549..30580
                     /gene="galR"
                     /locus_tag="J1138_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBA8453328.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30587..31849)
                     /gene="lysA"
                     /locus_tag="J1138_001513"
     CDS             complement(30587..31849)
                     /gene="lysA"
                     /locus_tag="J1138_001513"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBA8453329.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAAEGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31971..32906
                     /gene="lysR"
                     /locus_tag="J1138_001514"
     CDS             31971..32906
                     /gene="lysR"
                     /locus_tag="J1138_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBA8453330.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHSENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32893..33585)
                     /gene="ygeA"
                     /locus_tag="J1138_001515"
     CDS             complement(32893..33585)
                     /gene="ygeA"
                     /locus_tag="J1138_001515"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBA8453331.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33714..35132)
                     /locus_tag="J1138_001516"
     CDS             complement(33714..35132)
                     /locus_tag="J1138_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBA8453332.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8718 a         9620 c         8669 g         8275 t
ORIGIN      
        1 atcatcagga agttgaacgc tgaattgctc attaatgata gttaatgcat cgcgacttat
       61 cgcatactct gctttgtaca acgctttgat ttcatcaagg aaaacattat taatttgtat
      121 acctgaaacg actcgggata ttgcattcga aatgtgatcc gcaagaatca atagtagcga
      181 tggattgaga tttttttcga gatttttctc cgcatattgc actatttttt cggcaacaaa
      241 cacatactca atatctacat gttcaatcaa tttataaagt ttgttttttt gttcatttct
      301 gacataaaaa atccggttag ccggatgctc agggaccgac attccatatt ttttgttgta
      361 accgacgcca ggcccagaaa tgataacctc ctgtccattc atcgatgcct gtacacaatt
      421 attgttcatg actttttcga tgatcattcc atggctccaa aaaaaaaggc aaggcacgtc
      481 agaagtaata actacttcaa acattgcctt gcctgattta acagttacaa gatgattaac
      541 taatgcgtat acattacacg gaattgtgct taattaaaga gatggtcggc ttatttttca
      601 agccgacatt caaatatccg acatcagtca caaaaccagc gtctaatatt caggtaaatg
      661 ttatctattc aacgaaatat aaattcattg aataaataaa aacattatcc ccttctcgcc
      721 agcgtcgccc catcggcaaa atacgcttta attcccgcaa gaatagactc cgcaacttcc
      781 tgctggaaag tcgccgtttt cagtttacgc tcttcctcaa cgttactgat aaacgccgtt
      841 tcgaccagaa tggagggaat atctggtgcc tttagtacgg caaacccggc ctgttcaact
      901 tgatttttat gcagcttgtt gattttaccg agcttattca gcaccgcttt accaaactta
      961 aggctgtcgg caatggtcag cgactgcacc atatcgaaca tggtgtggtc gacatagcgg
     1021 tcaccgcttt tgcttacgcc accaatcaag tccgaggcgt tctgggtttg tgccagatat
     1081 tttgccgcag tactggttgc gcctttggtt gagagcgcaa acaccgagga accgctcggc
     1141 tggcgactgg taaaggcgtc ggcatggata gaaacaaaca agtcagcgcg ctgtttctgg
     1201 gcttttgcta cgcgcacttg caacggaatg aagatgtctt cattgcgcgt catgtacacc
     1261 ttcatattgc cctctttctc gatcagagag cgcagacggc gagctatttg caataccacg
     1321 tctttttcgc gcgttttgta tttccccacc gcaccggagt cttcgccacc gtggccaggg
     1381 tcaagcataa tgacaatcgg acgatcccgc ccagctttac ccggttgtgg accactttgt
     1441 gctggcggca cctgcttttc gaggtcgcct ttgttgtaat cctccagcag cgccagcagc
     1501 gggtcctgca tatcctgtgc attggccgga tagaggtcca tcaccagacg ctctttaaac
     1561 ccggcgaccg gcgcaagggc aaacagctgc ggttttacgt tttgctttaa ttcaaaaacc
     1621 atacgtacgg tttgcgggtc aaattgcccg acgcgcgccg acttgatgaa cgggtcgtca
     1681 gcgcggattt gcgcagccat ccccttgagc accgagttca ggtttacatc ttcgatatcc
     1741 accaccacgc gttcaggatt actcaacgcg aactgcttat atttcagctg acgatttgat
     1801 tctaccgtca cgcgggtgta gctggacgca ggccagacgc gcaccgccac gacctggctg
     1861 accgcagcca ggctgacctg acttacgctc aatagccaca tggcacccgc gccttgcagt
     1921 aaacgacgac ggctgattgc agtgttggat cctgacatgc ctctcccgag caaaagaaat
     1981 ctaagctgtg taacaagtaa acgaccaatt tgaccggaaa ctttagcgaa agacgcataa
     2041 tctgtcatct ataaaacggt aaacattctt tttatattca cggtattact gataaaaaac
     2101 tcgctcccgc ataaaattta cacttgcacc ccgcgaaaaa acagaataaa aatacaataa
     2161 tttcgaataa tcatgcaaag aggtgtaccg tggtaaagga acgtaaaacc gagctggtcg
     2221 agggattccg ccattcggtt ccctatatca atacccaccg gggaaaaacg tttgtcatca
     2281 tgctcggcgg tgaagccatt gagcatgaga atttctccag tatcgttaat gatatcgggt
     2341 tgttgcacag cctcggcatc cgtctggtgg tggtctatgg cgcacgtccg cagatcgacg
     2401 caaatctggc tgcacatcac cacgaaccgc tgtatcacaa gaatatacgt gtgaccgacg
     2461 cccaaacgct ggaactggtg aagcaggctg cgggaacatt gcaactggat attactgctc
     2521 gcctgtcgat gagtctcaat aacacaccgc tgcagggcgc gcatatcaac gtcgtcagtg
     2581 gtaattttat tattgcccag ccgctgggcg tcgatgacgg cgtggattac tgtcatagcg
     2641 ggcgtatccg gcggattgat gaagacgcga tccatcgtca actggacagc ggtgcaatag
     2701 tgctaatggg gccggtcgct gtttcggtca ctggcgagag ctttaatctg acctcggaag
     2761 agattgccac tcaactggcc atcaaactga aagctgaaaa aatgattggt ttttgctctt
     2821 cccagggcgt cactaatgac gacggtgata ttgtctccga acttttccct aacgaagcgc
     2881 aagcacgggt agaagcccag gaagagaaag gcgattacaa ctccggtacg gtgcgctttt
     2941 tgcgtggcgc agtgaaagcc tgccgcagcg gcgtgcgtcg ctgtcattta atcagttatc
     3001 aggaagatgg cgcgctgttg caagagttgt tctcacgcga cggtatcggt acgcagattg
     3061 tgatggaaag cgccgagcag attcgtcgcg caacaatcaa cgatattggc ggtattctgg
     3121 agttgattcg cccgctggag caacaaggta ttctggtacg ccgttctcgc gagcagctgg
     3181 agatggaaat cgacaaattc accattattc agcgcgataa cacgactatt gcctgcgccg
     3241 cgctctatcc gttcccggaa gagaagattg gggaaatggc ctgtgtggca gttcacccgg
     3301 attaccgcag ttcatcacgg ggcgaggttc tgctggaacg cattgccgct caggcgaagc
     3361 agagcggctt aagcaaattg tttgtgctga ccacgcgcag tattcactgg ttccaggaac
     3421 gtggatttac cccagtggat attgatttac tgcccgagag caaaaagcag ttgtacaact
     3481 accagcgtaa atccaaagtt ttgatggcgg atttagggta agcgatgaaa atcgccggat
     3541 gcgacatgcg taacactcgt gcgtcgcatc aggcaattac gtttatcccc gtgaactaaa
     3601 caacgccgcc agaccactgc gccgctcagt acgagtggcg attgccgcac ttaatatgcg
     3661 ctcatcggca tacagcgaca gacggcgacg cgcgcgggtc accgcggtgt aaaccagctc
     3721 tcgcgttact accggcgtgc gttgactcgg taaaatcaac gccgcatggt cgaactccga
     3781 tccctgcgat ttatgtaccg tcatcgccca cgtcgtttcg tgctctggca ggcgactcgg
     3841 ttgcacagac ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc cctgcccgcg
     3901 atccagcgca ataccaatat cgccattaaa caacccaagc gcgctgtcat tacgggcaat
     3961 catcaccggt cggccttcgt accaacgaga gtgcggatga cgatgaattt tgcgcttctg
     4021 ttgcataaac tgctcaattc gctcattcag tccagccacg ccaaacggcc cttcccgcag
     4081 ggcgcacaaa agctggtact cattgaacgc ctgaatgatt aaatccggct cggcacgcgc
     4141 ttgcagcaga tccagataac gtccgtaacc cgcaagagct tcctcaagca tcgcaatata
     4201 atcttcgccg ctctgtaaaa gtcgtttttc gatatcagta aaatcctgct gaaaaacggt
     4261 tttcactgcc gttttatcac cacggttaat cgccgcagct aactgaccaa tgccagaatc
     4321 gctgccgaaa cgatagcttt tttgcagcag gcagaggctg tcgcgcaaag atgccgcttc
     4381 tgtgccagtt cctgctggaa cgtggcttcc cgtcaggcgg ctcaactgcc cggcacgctc
     4441 ggcggtaaag cccgcgttgg cataagcgca gatatcgccc agcacagccc cagcctcaac
     4501 cgaggccagt tgatcacgat cgccgagaaa gatcactcgc gcatgatcgg gcaaggcgtc
     4561 gatcagtctc gacatcatag gaagatcgat cattgacgct tcatctacca ccagcacatc
     4621 aagatgcagc gggttaccgg catgatgacg taaacgctgg ctacccggct gcgcacccag
     4681 caatcggtgc aaagtgctgg catcttccgg aatgcgtttc ttttgttcat cggtcagcgg
     4741 taattgtcgc aaagccttgc cgagagattc ggttaagcgc gcggcagctt tacccgttgg
     4801 tgcagccaga cggatacggc agcgttcgcc gtcggccatc tgaattaagg ctgccagcaa
     4861 cttcgctacg gtggtcgttt taccggtgcc aggaccgccg gaaatcaccg aaatccgccg
     4921 cgtcagcgcc actgccgccg caactttttg ccagttaatt tcatcgctta ctggaaaaag
     4981 tttgtccagg gtttgcgcca gtagagcttc atcaacctca atggcatgat tcacttcgtt
     5041 gaaaaagcgt gccactgtgc gctcgttaca ccacatgcga ttcaaataaa gacgatcgcc
     5101 acagaggatc atcggcgttg gttcatctcc ccggctgacc gcttgtgaag ccagcaagca
     5161 ttcttcccaa ttttgtagtt caccgatttc actgacacag gtcgccaaca gcggatgcga
     5221 tgcctcgtta ttttccagtc gtgaaagcgg caaacaaacg tgtccctctc cggcatcatg
     5281 acttaatagt gccgccgcga gggtgacggc aggatgttca tctcccgcca cggtcagggc
     5341 aaactgcacg tccagcgggc gtagctgttt gtgctccaca gcttccagta attgcttttg
     5401 caatttcatt acgcctcctc cagggtcata ctggcaaaca tctcatccat caggtcaatc
     5461 aacccggcgt tgggtcgggt cgcgtagatc ccttgttgcg gatgttcttt atcaacgcca
     5521 cgcaggaaca gataaataac gccgccaaag tggcgctcat agtcgtaatc agcaatacga
     5581 tggcgcagat aacggtgcag cgccagggta taaagctgat attgcagatc atagcggtgt
     5641 gcctgcattg ccgctgccat agcctgttgg gtgtaagccg aactgtcttc acccaaccag
     5701 ttggatttat agtcgagcag gtaataacgc ccttcgtggc ggaacaccag atcgataaag
     5761 ccttttaaca tgccacgtac ctgcatgaac tccagcggcg ggcagccagc ggatagcggg
     5821 tcaaactggc gaattagtgc atcaagctgg ctggcgatga gcggttcgct aattggcagg
     5881 taaaactcca tctccacctg tttatcgcga tcggaaagct gactcaggct aatacccgtt
     5941 tcattgagag gtgccaggag gacagttgtg atccactcag tcaacaccgg ttcccactgc
     6001 ggttcaaagc caccgagctc cagtttttcc tgtacccagt tcgggtcaac cggctgggta
     6061 aaatcgaggt cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc gcgcgggaac
     6121 tgatgcggtg ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc atcaacatcc
     6181 agccgtggca tcagatcctg agcgataccg tgaccacgct gctgcaaacc ggagtagctg
     6241 gtgacacgcc agttatcgcc gggcaatcgt tgtagcgtcc tcgcattcag ttctgcagtt
     6301 aatgcatcat taacctgcca gggctggtta tcaccaattt gtgccgtttg ccaggcaata
     6361 tcatcattgc acaacgcttc aatacaggta cgaagccctg ccgcatcttg cggttctcct
     6421 ttttgcagca aacgcccgag cgcactttgg tggacgtcgg tatcaccttt tttatcgcca
     6481 cgacggcgca ccagcggtgc aacgccgaga ctgcaatgcc aaactgaacg cgtcagcgcc
     6541 acgtaaagta aacgcagatc ttccgccaga cgttcggcct ccgcgaggtc gacgctttct
     6601 ggcgcagcat taagatccag aacggcctca aacgagtggc gatcgtgata aaacgcctga
     6661 tcctggacgc ggaaattggt aataaatggc aaccagacca gcggatattc cagccctttt
     6721 gatttgtgta tcgtgacaat ctgcaccaga tgtttatcac tttcgaggcg catttgttgg
     6781 ctggaggcat tactgtctgg ctcgaggata tgttgcgata accagcgtac cagcgcatgt
     6841 tcactttcca gctgcgttcc ggcttcttgt agcagttcgc tgatatgcaa gatatcggta
     6901 agacgccgct caccgcctgc cgttgccagc aagttttctg caatgttacg cgccgacatc
     6961 agcgcccgca gcatcggcat aacgccacgt ttgcgccaga tttgccgata accatcgaac
     7021 tcttcgacga ccgcatccca cgcatgttcg tcattattca gcgtttcaat atccagcgca
     7081 ttcagcccca tcattgacgt tgccagcgca ctgcgcaggg tgttctcacg ttcgggcgtc
     7141 atcaccgcct gcaacagcca aagcatctcc tgtgcttcca gagtttcaaa aacactgtcg
     7201 cggttcgaaa ggtaaacgga agggatttcc agcagcgtta aggcatcgcg cacctgagcg
     7261 gcctcctggc ggctgcgcac cagcacactg atgtccgaag cacgcaccgg acgcgcgtcg
     7321 tcgccgttca tcagcaacgc ttcgccgcgt tgcccggctt gtaaccagtc gcggatttgc
     7381 gcagcacata cctgcgccat ggtactttga tagtcgccaa cgccgcagct ttcgccttcc
     7441 atcagccaca ttttcatcgc aggctgcgtt tcacctttaa acacaaaacg taacgcctga
     7501 tttttgccgg cagatttcac aggaataaac ggtatttcac gaaacatgaa cgcgtcatca
     7561 gtctggctga aaagtttgtt aacgctgttc accattcccg gtgcggaacg ccagttggta
     7621 tctaaggtgt agtgagcatg aacttcgcta cgcgccttca tataagtgaa gatatccgca
     7681 ccccggaatg catatatggc ctgtttcggg tcgccaatca gcaacaatgc ggtttccggc
     7741 tgatggtgcc agatacggcg aaaaattcga tactgttggg ggtcggtatc ctgaaattca
     7801 tcgatcattg ccaccgggaa tcgcgtacgg atcgccgctg ccaatacctc gccgctttcg
     7861 ctacgcagcg cggaatcgag ccgacttaac atgtcatcaa aacccaattc gccacggcgg
     7921 cgtttttcac gcgctactgt ttcgcggatc tcagccaatg cgcgggtgat caccagatcg
     7981 cggatcgaca atggttctgc aagcagttga tcgatcgcct caaacagtgg atgtcgcggg
     8041 gtttcccccc cggctttcgt gcgatcttct aagaaacgct gagagaattt ttccagcgac
     8101 tctggcaact gataactgtt ggtctcttct tctgcccagg cgctgatctt gtcgatccat
     8161 ttagcctgat tgctacggtt aaacttgcgt cgatcaatac cagaagattc gatcagcgca
     8221 tccagttcac ccaccgcgtc gcgccactgc tgttttacgg cgtcgatacg cgccacaatt
     8281 tgcgcgtggc gggaagccag cgtttcatca tcgggcggcg gtgctttgat aaccggtgct
     8341 tcgccttgca gataacgatt aatatcgcgc agcaacgcct gcggcccttt ccaggtttca
     8401 aagaccacct gggcaatttc acgcgtcagc gggtagcagt ggcgacgcca gaaatcggcg
     8461 caggcctggt agcgtagcag agactcatct tcaatcagct gctgctcaaa cagcatgccg
     8521 gattcaaagg cattcaggtt gagcatgcgc tggcaaaaac cgtgaatagt aaagactgcc
     8581 gcttcatcca tctgccgttc ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg
     8641 atctcttcca gcagacgtgc atacagcgga ttgtcagtgg tttcacgcag gcaggcgata
     8701 cgcaactcgt gaatattgct acggatacga ccgcgcaatt ctgccgtggc agcctcggta
     8761 aaggtgacca ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc ggaaccgcct
     8821 agcccaagta acaggcgcaa atagagcgct gcaatcgtaa aggttttgcc tgtgccggca
     8881 gaggcttcaa tcaggcgctc accctgtaag ggcaagcgca aaggatctag tgtctcggcg
     8941 acatcactca ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc tgacgttctc
     9001 ccacactttc cagccttcag gatgcacata ttcggctttc ccgttctggc tgccggaaat
     9061 ctgcgacaga atagccatac cttgcggctc gaccaccgcc tgatggaaga aatcagcaag
     9121 tttttgcggc gtcagcagtt ttatctgggc cacgatttta tcacgcgaat cgaagcgcat
     9181 attgccgcga tcgaaatctt tacttaactt cgatgcttct tcgccgagcg tttgcggtgc
     9241 ctgcagcatc tgggttatta ccgcctgctg gatttgcgca aactcttctg gcttcatcgt
     9301 ccgcaatttt gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg
     9361 ctgtttatca ttgctttgca aaaggaagcc catgccccac tgacgcccca cgctcattgg
     9421 aaacgcaaac acggcatagc ccaactgttc ttcggtacgc aactgattgt agaaccacgg
     9481 ctgtacgatc tgccccaaca gagagctata ggctgagctg gtgtattcat cgtagccagt
     9541 cggtacaaat atcgctgcca gtgcggagtc ggtgctgtta ccagcttttt caaagatgac
     9601 ggattgtttt ttatcgacca cgacatcttt gttacgacac cactctgaac catcagcgcc
     9661 caactgtttt tgcacatggc gtgccagcgt tgttgcctgg gcttcagtca tgttgccgat
     9721 aaccataaac tctggtctag cccctgattt taaggcgtcg cgataggcca gcacctcttt
     9781 caacgtaatg gagggcaaaa ttttgcgccg ttcatctcgc gagaagtacg gcacttgcga
     9841 gagcatctgc gcgggcataa tcgcctgctc aaacgctttg cccttttctg cggaatccat
     9901 catctggtta taccaggact tcgcctgctc aagctgatct tccgtagcgg tatagctaaa
     9961 gtagccctcc agcagagcct ggaacagctg cggcaggcgc tgagtgtaac cattggcatt
    10021 aaccataagg ccgttgttag cattggtgga aaaacttatg ccaccaaccg acgcctggtt
    10081 gcttaactga tcaagcgcca gccctgcgag ataatcattg agcgcaaaca tcacctgatt
    10141 gcgggcgctg tccatggctt tcggattacg caaaatcagg ctgacatcag ctttgggctc
    10201 actggcaaaa taacggcttg gcgcatacac cacgcgcaga ttcgactcat caacaatcaa
    10261 ctctggatgg tcgtatttct tctctgactt aatcagcgag aaatcgtcag gaatataggg
    10321 gttaagctcc ggtaaggaga gcgcaatatt ggcggctttt ttctgccagt ccgcgaaagt
    10381 ttgttcgctg attttatcga cttgatacgg cgcatcgaca aaataagccg ttttattgtg
    10441 cggctctttc gggctgatat accagatacg cgcattctgc ggcgtcatca tcgccagacg
    10501 ttcttttact gctttagcat cgtaccgatc ggcaatattg actgcatcca gtgtatgctc
    10561 aacaggaacg cgaatcatgg tatcagccag ccattcgacg taatccatat cacgggtgat
    10621 tgacggataa cggaagtcga tatccagcac attcgccagt tcatcgaagt attgtttatc
    10681 gatccctttt tcacgtaaca gattgagata gctaaaaatg gccgccacaa cctgatcgcg
    10741 attcgccaga cctttatcgg ttaaagacgc agagatcgct aatacgccgc tgttgccgtt
    10801 gacgatagga tcggaattgg cgctaatacc ctcaactaat ccctgctttt gcagccagtc
    10861 agaaagtgta ccagggctgc gattaccaat cagataggta atcaattcat ccgttttact
    10921 acggaacttc gctgaattgt tatcgatgcg aaactcaacg cgcagaactt tacgcggcaa
    10981 cgccgggacg taatgaatga taatgccctt ttgcgcgtcg gtgactaccg gcacggtgat
    11041 ttccggtttt ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg ccattttcgc
    11101 caactccggc aacggtttat tgctgtaaat aaccgccttc atcagattgg cggaatagta
    11161 cttctcgtgg aaatctttca gcgcctgctg taccggatta cctggtttgt cgcttaaagt
    11221 ttcgaggtta ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg tttctgcgct
    11281 gacctgtgcc atgcgcatcc cgtcacgcgt acgcgccatg gttaattcgg cattcacggc
    11341 attacgttca cgttcggcgt atttcttgtc gagcaagggt tctgcaatag cgtccgccag
    11401 gcggtctacc gcaccgggca aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata
    11461 cggtgccgtg ctggcattgt gactgccgcc gtgcattttg agatattcgg ccagactgtc
    11521 agcctgcgga tactttttcg accccatcag actcatatgt tcaaggtaat gtgccagccc
    11581 ctggtacgcc tcaggatctt ccagcgaccc aacgggcacc accagcgccg agagcgattt
    11641 aactgcctgc ggatcagaaa ccagtaagac caccataccg ttatccagac gtatagcctg
    11701 atactggcgg ttatctttat cacttttacg gatggtttcc tgaatcggct gccatcccgt
    11761 ttctgcctga cttaagggtg cccagagggc aacaaacaac aataatgctt tgaaccagat
    11821 gctgcggggc attcacggac ctcataatca actttatttt ctggccagat tcaacacgtt
    11881 aacgcaaatc atctgccaga atttaatctt gtgctgaacg agtcagccta tgtctatata
    11941 accatcagtc cgtgactggt gcgcatcata aagtaagcgg atagattgcg caatttttat
    12001 acaatactca tgactgatta aagcgaaaca gcggtaacag gaaacgttgc gactgttcaa
    12061 cgatagcctc cattgtctct ggtgttaatt gccgccagag ccgttgatac cagatatcat
    12121 caccttcgcc acgcaccatc atgttgcctt cgtaagcctg aaggaatttc gtacgggctt
    12181 tttgcaacgt ggaatcgtca tccagcatgg catcgttttg cgcgtcataa caggttttta
    12241 gccacgcgcc gccactttca ggtaacacca gcaatggcgc ggacattcct tcacgatacc
    12301 cttcaatcag ctgtgagagg taatgtaaag cctgttcggc tgcaagcggc ggaaaacgcc
    12361 actcgccgtc tttgcgtaga aaaaggcgac tttcaccatt accaccgctg gcacagtaga
    12421 caaggtgttc cagccaaagt tgcattccct gcgccacact taataaagag ggacgccagc
    12481 gcaacaggcc atccggctgc acctgcggca accagccagt tatctgcaca ccgttgcagg
    12541 cgagatcgat ttccatgctc tgccctggct ggcgacaggc aatgactctg tcggcaagtt
    12601 gctgcatctc ctgacactgc gtttcccaga aaatttcacc aaaggcacca tacggcaaat
    12661 cacctgccgc ccggaatcgg cggaacaagc gttcggcatc atcctgctca accagtacat
    12721 ttaataactg ctggttgatt tgataacgac taagcccttc aagaataaaa ggttcggtgt
    12781 caggaatttc gctgtcttcg gtacggaagt tcacctgcaa acgcatctgg aaaaacgccc
    12841 gcaccggatg tgcccagaat cgttgtagcg tctccagcgg cacagtttcc ggcaaggtaa
    12901 acggcagcgg ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc
    12961 actcgcgcgc atagctttgt aagttaccgg gctggtagtt ttgcgggtca aacggcatcc
    13021 gggtatggtg gcaagtaaga tgcgctttta cccttgcctc gctttcatca cagttaagcg
    13081 cttcatcgcc cggtagataa tggctttgcc cgatgtagtc gatcagttcc tgcaccagta
    13141 ccgacgggaa acgttcactg ttatcctgaa tggaacgccc gatatagctg atatagagtt
    13201 tttgctgcgc ggaaattaac gcttccagga acagatagcg gtcgtcatcg cgacggctac
    13261 ggtcgccacg cttcggtttc tggctcatca gatcaaagcc caatggcgca agctgacgtg
    13321 gataaacgcc gtcgttcatt cccagcaggc aaaccacttt gaacggaatt gaacgcattg
    13381 gcatcagagt acaaatgtta accggtccgg cgagaaaacg ctggctgata cgttcttgat
    13441 ccaggcgctg tgccagttca tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg
    13501 cacctaaacc ttcggcgata atcgcctgcc attgttgttc gatcagcgtc atcgctgctt
    13561 cggtttccgc atccggcagg aagaaggcgt tgagcatatc gcgacaaacc ggcaaccact
    13621 cttccagcgg acgctcctgc gccagcccgc gacgccagat gtttagctgc attagcagtg
    13681 aagacagatg ccccaccagt tctgcaatta agccgctcga ttcatcataa ggtagaaccg
    13741 attgccactc gccctgcgcg ctctccatcg cgtagcccag caacatgcgc gtcaggccaa
    13801 accgccaggt gtgttgaccg gtagcgggaa gttccagctc gcgaacgttg tcgtcatcta
    13861 tcccccaacg aatgccggat tcgttgaccc actgacgtaa ataacgcagc ccttcttcgg
    13921 tgatgtcaaa ccgcgctgcc agcaccggca catccagtaa tgccagcacg tcttccgaca
    13981 caaagcggct gtctggcagc gataacaggc tgataaacgc ttcaagtaca ggatgcgact
    14041 gccgcgcccg acggtcggaa atggcgtaag gcaggtaacg atccgcaggt gcactaccaa
    14101 acacagcctg aataaacgga ctgtagctgt cgatatcagc caccatcacg atgatgtcgc
    14161 gcggagtaag tgtcgggtct tcctccagca tcgccagcag gcgatcgtgt aaaacttcaa
    14221 cttcacgctg cgggctatgg caaacgtgga aggtgatact gctatccagt ggatcaagcg
    14281 ggcgtttgtt atcgctacgg gaaaactctt cgatgttcac accagcaacg gcgcggtttt
    14341 ccagttccag aatgtcagac tgaatattat gcagcaggtt atctggcgtc acatcgacaa
    14401 aagcgtccag ctcctggctg ctctccaggt cagaaaggag ataaatgtag tcgcgcccaa
    14461 gcttgcccca tgaagccagc agcgggttgc cgacatcctg ttcaccatcg ctgttaaaga
    14521 gctgcccggc attttcgctg tcgcgaaata gcggtaattc gcgatcttca aaactgtgtc
    14581 ggcgctggcg agtcagtagt ttcgccagat aagctggatc tttaatgtcg ccccagtaat
    14641 aacggcaggg gttggtaaac aggagatgga tttcaatatg tttacccagc gcctgtagcg
    14701 cctggagata aacaggcggt aacgcggaaa taccgcatat aaagacgcgc gaaggtaacc
    14761 ccggcgggca ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag agattggcgc
    14821 ggtgccagcg cggttgccca agttcgtcgg tatattccac caacgccttc cacaacggcg
    14881 cttgccaggc ctgtgcttct cccaaccctt ctaccagatg tcctgtttcc cactgtgcca
    14941 gccagtccgg acgatagacc agatactggt caaacaggtc cgccgctttt gaagaaagct
    15001 ggaacagttt tcgcttgtca ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt
    15061 cgcgctccaa cagttgcggc agcagagtca tcagtttcca gctcatgctc tgtttgttaa
    15121 aggcgctctc tttggggatc tccggtaaca cccggacgaa catatcccag ataaagctcg
    15181 ctggcagcgg aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg gtcatttgca
    15241 gccactgtgc cataccggta ctttgcacca gaatcatctc tggttcgaaa ggatcgtcca
    15301 gccgttcgcg ttcgacaata aactccatca acgcttccag cacgtccaga cgattggaat
    15361 ggtagaccct taacatagcg gctcctgact actgacgatt cgggcaatgc aggcgggtca
    15421 tctcgccttc tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca catcccgcct
    15481 gcgatgtctg cattcggttg acctgccagt tagcaggtgg cgaaatcgcg cgcagttgcg
    15541 tttgctgcca gccatgccgc cagagctgtt ggtactggtt tctgctggca agactgttca
    15601 ttaatgttcg ctggaaaccc gataacgcag tgacaatcat caccatcaac accatcgcca
    15661 acattacctc cggcagacta aagccttgtt gattcctcag ggaagctgac ataacgccct
    15721 ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa aaaacaatgt ttccatcgat
    15781 gacttcgcct gttcgccaca acgaaacgcc ttcataacca gcaatcaata aggcttcatt
    15841 atctgccagt aaacgcagac aaacccgggc accggtggca gcatacagta agcactgaac
    15901 tgcgggctgc gtctgccagg agtgcatttt tccccactcc agcgccgact gaacgatggc
    15961 ctggcggcgc aatgactgac tttccatgct cacgcgagaa gcaaaactgc gatcctgctg
    16021 actcattcct tgcaatagca agctacccaa aatcagcagc atcaggacca gagccagtga
    16081 cgaaacaccc ttttcgcggt tcacaggttg gatcctgtca cgctatagct ggcatccacc
    16141 acggtttgcg gatcagcctt gctggcagcg tgcatattaa ccgtcaacac cggcgagaag
    16201 ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc cggattagtg
    16261 actttatccc agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc cagcacatgc
    16321 tccttcagac gaaatccaat ctggtcggac tcttttaccg gttcgcgatc ccagatacca
    16381 ttactgttcg catcccactg cacaatgaca cagtcaccct gtccgacaat ttccagccct
    16441 tcgcccgtac agttgccatg gcaataaccc gccctctgga gatgcttcgc gacggtaaat
    16501 acccgcagcc agatttcatc ttccagcgcc agcttacggg tgttcgttaa actttcacgc
    16561 tgtaacgcag gcagaaagcg tgcagccccc agcaacaata cgctactgat cgccatagca
    16621 atcaacactt ccagcagaga aaaaccttgc tcttttacag gcatccttct gtttctcctt
    16681 gctgacaaag ccggagtctt ccccacggcg aaaccaccag ccaccactcg cccgttgagt
    16741 ttttgaagcg aatatgcccg gcccatgcgg tattgcgcag gccaaagaaa gcaagcgaag
    16801 gtgtcaggtc gctcatttcg acttcgggcc agcgcggcac aaagaccaat ggtgaactgc
    16861 catgacaggt attcgcccca gcaacggaac tcacaaggca ccataacgtc ccctccctga
    16921 taagactgat actgtggtcg cggttatgcc agttggcatc ttcacgtaaa tagagcaaat
    16981 agtcccgcgc ctggctggcg gtttgccaaa gccgttgcga ctgctgccag tattgccagc
    17041 cataaagccc acttgcgctt agcatgacca gaatcagcat cgcgaccagc gtttcaatca
    17101 gcgtataacc acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa aaaagacgag
    17161 ggccagtttc tatttcttcg gcgcatcttc cggactattt acgccgttgc aggacgttgc
    17221 aaaatttcgg gaaggtgtct cgaagaattt aacggagggc aaaaaaaacc gacgcacact
    17281 ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aatgcccgga
    17341 tgtggatcgt atccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt
    17401 ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt
    17461 tgatccatat ggttgctgta gagatgcgtg tcgccaccgg tccagacaaa atcacccact
    17521 tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac
    17581 ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct
    17641 gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct
    17701 acgttccacg ctgaaacaat aatgcgacgg gaatccgggt cgtttttcag ctggttcagt
    17761 acagtagtga tctggtcaat atgacgacca tccggcgttg gccaggcgcg ccactgttta
    17821 ccatacactg gcccgaggtc gccgttttca tcggcccatt cgtcccagat ggtgacattg
    17881 ttttcgtgta gataagcaat gttagtgtcg ccctgcagga accacagcag ttcgtggatg
    17941 atggaacgca ggtggcaacg tttagttgtc accagcggga atccatcctg caggttaaaa
    18001 cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg
    18061 ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg
    18121 tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc
    18181 gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccagt aaactgcgcg
    18241 tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtaaccaat caggaacaag
    18301 cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc
    18361 accacacctt ccagcagcag ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa
    18421 gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg
    18481 gagccaggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg
    18541 ttaataaagt tgcccaggcg cccagcacca agaccaaacg gaatgagtgg ggcaataaaa
    18601 tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata
    18661 acgccaatca ggcctccgtg gaaggacatg ccgccgtccc agacacggaa aagatacagc
    18721 ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg tccaccgagg
    18781 aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg
    18841 cccggacgat tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc caccagatac
    18901 atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc
    18961 ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag
    19021 agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc
    19081 acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat
    19141 aaccccccgc gaatcagccc gcccatgccg cgacgctcca taaaggcagc aacctgatgg
    19201 cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcagca
    19261 aaatcaatgc gccgcagcag gtattttacg cgcgctacag aacgtccgtt catagacaga
    19321 tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg
    19381 cacaaacgga gatcgattcc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt
    19441 aacattgcag gatgaagact gtcataaatg ttcgccaccc gggtattgtt gcgatcaacg
    19501 gccaggatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc
    19561 agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg
    19621 ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt
    19681 gcttcgtcaa cttcatcgag gcttgtgacc atcggcaaca gaatattcag gttgcccgta
    19741 gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc
    19801 gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctaatcgg catgtaaggc
    19861 agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc attaaacatt
    19921 tgcagcatcc cctgatactg cgccacctgt tcttcttccg acggaaaacc actttgcagc
    19981 atgaatggga tttcagtgcg ataaagtcct atgccatcaa tacggctgcc cagtttttct
    20041 tcatgttccg ggcttaaacc ggcattgagc atgactttaa tgcgttcgcc gctttttaac
    20101 tgggcgggta aattgacgtc atcttccgcc agacggctaa gctcgatctc ttcactaatt
    20161 agccgctgat attcttgcag cagtaccggt tccggatcga ccagcaattc accgcgataa
    20221 ccatcgacga tcagcgtccg acgatgcagt accgaaggct gaatatccgc gcccatcacg
    20281 gtaggtatcc ccagcgcacg taccatgatc gcagcatggg agttggcagc gccatcgcgc
    20341 acgacaacac cgactaagcg atcctggggc agctcagcaa gcgttgtcgc tgacagttca
    20401 tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga
    20461 tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta
    20521 tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct
    20581 gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt atccgaaagc
    20641 aggtgtgagt aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc agcaaagcgt
    20701 ttgctatagc ggcgaaactc gttagccgcc tcttccaacg ccccggtcag tcgttcgcgt
    20761 tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca
    20821 tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcacg gattcgcgtc
    20881 tggcgatatt gcccaaacaa ggcagtcaac tgcgactgag aaagaatagc tgccatctgg
    20941 gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc
    21001 tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg
    21061 cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct
    21121 gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg
    21181 agcgttacag tgcgaccgcg tggttttttc agtccccggg tcgccatcag gtagtaacaa
    21241 cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg
    21301 tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact
    21361 atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca
    21421 gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct
    21481 ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac tggataccag
    21541 taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct
    21601 gcatcgccgc tcaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc
    21661 ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa
    21721 gcaaggattc ggacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc
    21781 gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga
    21841 ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt
    21901 gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat
    21961 gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta cacactaata
    22021 acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg
    22081 tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa
    22141 cccggagcat tctgaataag acagatatag agattttaaa atattcatta ttagtcaatg
    22201 cattacatgt gatttgagta ataaaaaaga gtaattcgtg acccaggtca cacctctcat
    22261 ttacgtgttg gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt
    22321 aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag
    22381 tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt
    22441 ttattttcag tgaccagatt tggaaaaccc gttgcagtgt tgcgcaactc gtttaccggc
    22501 aacctaaaaa gcaatataaa tcagagaatt gaacaacgca tgtggaaaaa gttacactgc
    22561 gaatattcag cacataattg ctgtttgttt tttaaccaag gtatcatgac atgtcccaac
    22621 ctcgcccact gctctctcct cccgaaactg aagaacaatt gttagcgcaa gcacaacaac
    22681 tttctggtta tacattggga gaactggcgg cacttgccgg gctggttacg ccggagaatt
    22741 taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag
    22801 ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg
    22861 atagtcttgg tcgtccactg gaaacaacat tcgtttgtgt tgccccgtta acgggcaata
    22921 gcggggtgac ctgggaaacc agccacgtgc gccacaagct caaacgcgtg ctgtggatac
    22981 cggttgaagg cgagcgcagc atcccgctgg caaagcgtcg cgtaggatca ccgttgctgt
    23041 ggagcccgaa tgaagaggaa gaccggcagc tacgcgaaga ctgggaagaa ttaatggata
    23101 tgattgttct cggtcagatt gagcggatca ccgctcgtca cggggaatat ttacagatac
    23161 gaccgaaagc agcgaatgcg aaagcgctga ccgaagctat tggtgcccgg ggcgaacgga
    23221 ttctgacgct gccgcgcgga ttttatttga agaagaattt caccagtgcg ctactggccc
    23281 gtcattttct gatccagtag ccatcgcttt gacctgctgc tttccgggca tataattacc
    23341 gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc
    23401 ctggcttgcg ctcggtacac tgacgctgct ggagatcgtt cttgggatcg acaatattat
    23461 tttcctttct ctagtggtgg caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct
    23521 ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatccatcg cctgggttac
    23581 gcgcctgacg aatccgcttt ttacaatatt cagtcaggaa atttccgccc gtgatttgat
    23641 cctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat
    23701 cgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg cgatcgtgca
    23761 gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc
    23821 agatcacctg tttattatga tggctgccgt ggtgattgcc gtaggcgtga tgatgttcgc
    23881 cgcgcgctcg attggtgatt ttgtcgagcg ccatccttcg gtaaaaatgc tggcgctctc
    23941 tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa
    24001 aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca acctgattcg
    24061 caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc gtactcactc
    24121 ctgttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc ggtttataaa
    24181 cagactatta tcataggtgg gccaaatgag gataaccgga tgaaaaaatg ggcagtaata
    24241 atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg
    24301 accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg
    24361 ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg
    24421 caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt
    24481 tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa
    24541 cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg
    24601 gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg
    24661 ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc
    24721 ccgaaactca agggctaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc
    24781 gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaaaggca
    24841 tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca
    24901 agcgtctgca gactgattac ctcgatctct atcaggtgca ctggccgcag cgcccaacca
    24961 actgcttcgg caaactcggt tatagctgga cagattctgc gcctgcggtt tcgctgctgg
    25021 atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt
    25081 cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc
    25141 cgcgtattgt caccattcag aacccctaca gcctgttaaa ccgcagtttt gaagtaggtc
    25201 tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg
    25261 gcacactgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt aatacgctct
    25321 ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg
    25381 atatcgccag acgtcatggc ctggaccctg ctcagatggc gctcgcattt gtacgccgtc
    25441 aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggagcagctg aaaactaacg
    25501 tcgaaagttt gcatctggag ttgagcgaag acgtattagc tgaaattgaa gcggtgcatc
    25561 aggtttatac gtatcctgca ccataagatt aacgccggtt aaaaccggcg ttaaatatta
    25621 atgacggcgc tgccagatcc acagcgccgt tattgccagc gcaaacagcg caccgaagcc
    25681 aatgccaatg ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa tgcccagcat
    25741 caacaacatg gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc ccgccccgac
    25801 gcttttttta ccccgctcct gtagcaacgc attgagcgga acgacaaaaa agccccccag
    25861 cacgccaatc agcatcaaca aagcataggc tggcagcagc tcgtgttgca gggagaaaat
    25921 cagtaccacc acgccaatca aaatcccggc tggcatacag cgtgacacgg tttccagcgt
    25981 aactaacttc gctgccgcac ctgcaccaac cacgatacca atcgctacca tcgcgttgag
    26041 ataggtcggc gtagcgttat cggtaatacc cagcgccacc ggtacccaca gcaccaacag
    26101 gaaacgcagc gtgacacccg ctccccagaa taaactggtg cccaccagcg aaaaacgcgt
    26161 ttcaccattg cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgctgatgag
    26221 attccaggac tgccccggac gcgccgccgc cagtttggga atgtagatat tggcaacgac
    26281 cgcgccaccg taggccagtg cgcatgcggc cagggcaacc agaatatgcc agtcagccag
    26341 aacaccacca gctacggaac cgagcaaaat cgccgctatg gtagacgctt ccattaaacc
    26401 gttagctttt actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg
    26461 tgaataggct gcagcaccaa cacccaccaa cgtatagccg agaaacggat tgataccaaa
    26521 gcagatactg gctgcgccca gtagcttcag gccgttggca aacatcatca cccggccttt
    26581 ggcgaagcta tccgccacct gtccgacaaa cggcgcaaaa agaatgtaag cacctacaaa
    26641 caccatttgc aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag
    26701 agtggcaaac agcagcgcat tatcgccaaa cgcagagaga aactgcgcca cgataaccgc
    26761 tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt
    26821 ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg
    26881 gcatctgttt cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc
    26941 gggcatactg ttgtaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg
    27001 cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac
    27061 caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc
    27121 gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag
    27181 tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta
    27241 cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc
    27301 gtccgccctc ttcgatacca ggaaccgaca acaggcgcgc gtccatacct ggtagaatac
    27361 gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcact
    27421 cggtcacacc gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttag
    27481 tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg
    27541 ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt accgaacaac acggtgcaac
    27601 tgcggtcata caccaactcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca
    27661 cttttgcacc tgtgagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca
    27721 gcggtaacgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct
    27781 cgacattcgc cagaatgctt ttatggctat ggacgacgcc tttcggatgg ccttcagaac
    27841 cggaggtaaa aaggatcagc gccgcttctt ccggctgctg ttttacctgt gccagacgcg
    27901 gcatcagcaa atgagcgaaa atccacactt tgtcggcagt ggtgacatct gcttttaaat
    27961 cttccagata gacccagcgc acctgagtaa gttgctccgg cagatgccag agtttgcctt
    28021 tatcgagaaa ctgacgggaa gtgaagatgg ttttgatttc agccgccgta atagcactgg
    28081 tcaacccttt taccccggcg gtgtagttca tcattgcggg aatgcggcga cgggcgatgg
    28141 ccccaaaaat cactgccgca ctgatggctg cattgggcag cattaagccg atgcgttcgc
    28201 cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc
    28261 gataggagtc tggggtaaag ttgacgtctt cgacacattt cttcccggct ccgaagcggt
    28321 acattgcact cagtaaagat tcgtacagcg tttcacgcgg gcgcaccgcc atacgcgctt
    28381 ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg
    28441 catccggcat ctccacctgc gttggtggca aaatatgcag agtaatttgc gggaacaagc
    28501 gacgtttaac cagaccttcc aggcggctga agtgcgtaag ttccgcccct tcaatacgca
    28561 caggaataac cgttgcacca gacttcgccg cgacaaatcc cgcgccatcg tagattttca
    28621 tcagcgagcc agtggtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt
    28681 ccaccagacg taccagatgt ttaatcgcca taggttgcgt cgggtcgaga ggaacaaagt
    28741 cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa
    28801 ataccggacg tacgggtaaa aacaatgcca gcagtatgcc atcaataaaa gagacgtgat
    28861 taggcgtaat tagaacgcgc tcgcccttca gtgccttggt gtcacccgta acgcgaacgc
    28921 gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc
    28981 tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa
    29041 attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa
    29101 gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacga
    29161 cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa
    29221 ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc
    29281 tggtgtaatt catgtgctca acccgaagtt gacttcacct atcaatacct ctgggatcac
    29341 cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag
    29401 tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg gctcacattc
    29461 ccacaatgaa aacacgccgc cccttgaacc aacgggcgtt ttccgtaaca ctgaaagaat
    29521 gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag cccgactggc
    29581 aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag ccagcgaagc
    29641 ttcccggctt gctgtgcata gtgcaatgga gtctcttagc tatcacccga acgccaacgc
    29701 ccgtgcgctg gcgcagcaga ccactgaaac gatcggtctg gtcgttggtg atgtttccga
    29761 tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca ctggtaattt
    29821 tttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcca ttgagcaact
    29881 gatccgacat cgctgtgctg cgctggttgt ccatgccaaa atgatcccgg atgccgattt
    29941 agcctcgtta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt
    30001 tgaaaaccgt tgtattgctc tggacgatcg ttacggtgcc tggctggcaa cgcgtcattt
    30061 aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta tttctgacgc
    30121 cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattc cggccaatga
    30181 ccggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa tgaccgagct
    30241 tttgggacga ggcagaaatt tcactgcggt agcctgttat aacgattcaa tggcggcggg
    30301 cgcgatgggc gtgctcaatg ataatggtat tgatgtaccg ggtgagattt cgttaattgg
    30361 ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc
    30421 aatcgtgacg atggcgacgc aggctgccga actggctttg gcgctggcgg ataatcgccc
    30481 tctcccggaa atcactaatg tctttagtcc gacgctggta cgtcgccatt cagtgtcaac
    30541 tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa agcaattcca
    30601 gcgccagtaa ttcttcaatg gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa
    30661 acagaacttc tggtaacagc ggacggctgt tgtagttgga tgacattgat gcgccatatg
    30721 cccctgtatc atgcagtacc agataatcac ccgccttcac ttccggcaag gcgcgggttt
    30781 cgacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac ggcccggcga
    30841 cgacggtttc caccgttggc gcgtgttcca gagaacgacc ctcagctgcc agggcactga
    30901 tatggtggta actaccgtac attgccgggc gcatcagatc gttgaacccg gcatcaacca
    30961 gcacaaagtg gcggctaccc atttgtttga cgctccgcac ttgagtaatt aatacgccag
    31021 cctgcgctac cagaaagcga cccggttcaa tttccagttt cacaggatgg cccaaatggc
    31081 gggcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta tcaaccgcct
    31141 cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc tgtaaatcct
    31201 gaccgaactc gaggacctga cgcaccatag caccacatac ctgttccaga tgggcataat
    31261 cgacgccaga accaatgtgc atgtgaatgc cgaccagctg tagatgatga cgttgtatca
    31321 cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt tcgccaccgg
    31381 tattggtttt ttggctatgc ccgtgaccaa accccggatt aacacgcagc catacccgat
    31441 gccctggaga aacctgaccc agttggtcga gcatatcaac agaacccgca ttcaccggaa
    31501 tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa
    31561 tatcatcggg gtgcgtttgc ggattgtaac ccgccgccag tgcacgctct atttcgccta
    31621 acgagacgga atccactttt acgccctgct cacgcattaa gcgcaaaata tgaatattgg
    31681 aacaggcttt ctgtgcaaag cgcaccacat caaactgttt cagcgctgca atctgccgac
    31741 gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttctgcgggc aaacgcagca
    31801 gattttcggc ggtgagatcg gtatcagtgc tgaacagtga atgtggcata acaaactcca
    31861 gataagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata tcgatttatg
    31921 tcgagtctat gcaaaaatga tatggattac cggattgcga gagagcgcta atggccgcag
    31981 ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc ctgactgagg
    32041 cggcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg cgctttgaga
    32101 aggtgatcgg gctgaaattg tttgagcgca tacgtggacg attacatcct accgtgcaag
    32161 gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc attgtcagtg
    32221 ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc ctgccggtct
    32281 tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat cccgatgtca
    32341 gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc
    32401 atgatttagg actcactgaa acgctccata cgcctgcggg aacagaacgt accgaattac
    32461 tctctttaga tgaagtgtgt gtgttacctc cgggccatcc gctggcggta aaaaaggtat
    32521 taacgccgga tgattttcac agtgagaact acatcagcct ttcccgtact gacagctatc
    32581 gccagttgct ggatcaattg tttactgaga atcaggttaa acgacgcatg atcgtagaaa
    32641 cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggcgtt tcggtggtta
    32701 acccgctcac cgcactggat tatgcggcaa gcggtttagt ggtgcggcgg ttcagcattg
    32761 cggttccatt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc
    32821 aggcgtttag tgagcattta caagcgggat taccgaaact ggtcacttct cttgacgcta
    32881 ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga tcgccgcggt
    32941 atcaaacaca ggcagaacac tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc
    33001 aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg cataataagc
    33061 gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct gattaatttt
    33121 cgcccgttca tccgcttcag gaataagaca gttgatggaa aattgttccg tcagccgccc
    33181 gcgataaaaa tcctgttcca tggtgtaacg tgtacccagc agcgccacac gagtcattcc
    33241 tgccccggta attgcacgtc cagtggcatc cgctatgtgt aagaaaggca gagagcaacg
    33301 agactcaatg gcatccgcca ctttatgcat cgtattggtg cataacacaa taccttctgc
    33361 gcccgcccgc tgtaagccaa gcgccgcctc agccagaata tccccggttt tgtcccactc
    33421 accgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca gcacttgcgc
    33481 agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat agtaaggaat
    33541 agtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc tctcctgatg
    33601 tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc
    33661 aatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg aaatcagacg
    33721 ccgatatttc tcaacttttc gcctgccatc agtttgcgct cgatatgttc cagcgtgaca
    33781 tttttggttt ccggaatgag ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg
    33841 tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa ggtcgcgccg
    33901 ataatcatat tcgacaccca gtttgtcgtc gttgagcagg tgatgccaaa atcgcggcat
    33961 ttcagcggct gaatttcaga gcacaagatc cacaccactg gcgcggcgct catcgcataa
    34021 ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact ggaagccgta
    34081 ccgttatcaa actgcatcag gcagtaaccc agcaccagag tgcctaacgc catcacgctg
    34141 aaaccaattt tcagagccgg cttacgccct gctttatcta ccgtaaagac cgcaatgaag
    34201 gtagcgaaca taaaggtcag ccccacgacc agagtcgcaa tcatctgttg ttctgtggtc
    34261 gtaaagcccg ccattttgaa gatacgtggc gcgtagtaca tgatgatgtt cataccggta
    34321 aactgctgca tcgcctgcaa caacataccg agaaatacag cacgacggac gttacggttg
    34381 atcttaaaca gtgcccaacc gccctgtttt aacttcaggc tttcacgaat ttcgttgagt
    34441 tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc cgcctcaata
    34501 tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac cagaataatc
    34561 agcaggactg ctggtaaagc aagaaccccc aacattgcgc gccagttacc gctataactg
    34621 aacgctgtat cggataaaaa cgccagcacg atgccgagtg tgaccatcaa ctggtacatg
    34681 ctgatcatct taccgcgaac gttttcactt gccatttcag aaagatatag aggagcggtg
    34741 taagacgcga tcccaacagc aatgcccaac accacacgag cggcgattaa catctctacg
    34801 ctggtcgcaa aagcggaccc tatcgaaccg agtacaaaca ggatggcccc cgccatcagg
    34861 ctgtatttac gccccaggcg gaacgacagc cacccattaa acagcgcacc aattgctgcg
    34921 ccgagcatca tgctactgac cacccattcc tgcaaacggc tggtcagcac aaagtgatcg
    34981 gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa caatcctgcg
    35041 accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcccgcag aggacgtggc
    35101 gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag agtaagacat
    35161 agtgaaaaaa tacgtgaaca actcacacag gtgtcaggtc ggaaatagca taaatatgga
    35221 ttaaattgct gcgacatgtc gttatgtgat ggatattcca attttcaaat taagttgaat
    35281 ta
//
LOCUS       DADTFG010000033        35029 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.033, whole genome shotgun sequence.
ACCESSION   DADTFG010000033 DADTFG010000000
VERSION     DADTFG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.033"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            145..387
                     /locus_tag="J1138_001517"
     CDS             145..387
                     /locus_tag="J1138_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453333.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            502..1140
                     /locus_tag="J1138_001518"
     CDS             502..1140
                     /locus_tag="J1138_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBA8453334.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWTRTMGWTEYQQVRYATARSVFKWNGTDTVKVGSDET
                     PVRVLDEEVFTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1137..1874
                     /locus_tag="J1138_001519"
     CDS             1137..1874
                     /locus_tag="J1138_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBA8453335.1"
                     /translation="MIKQTIVALLLSAGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGTPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQILLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1874..3970
                     /locus_tag="J1138_001520"
     CDS             1874..3970
                     /locus_tag="J1138_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBA8453336.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPKYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLGGEP
                     LGHETTLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4017..4295)
                     /locus_tag="J1138_001521"
     CDS             complement(4017..4295)
                     /locus_tag="J1138_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453337.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4510..4920
                     /gene="psiE"
                     /locus_tag="J1138_001522"
     CDS             4510..4920
                     /gene="psiE"
                     /locus_tag="J1138_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBA8453338.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5014..5904)
                     /gene="malG"
                     /locus_tag="J1138_001523"
     CDS             complement(5014..5904)
                     /gene="malG"
                     /locus_tag="J1138_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBA8453339.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5919..7463)
                     /gene="malF"
                     /locus_tag="J1138_001524"
     CDS             complement(5919..7463)
                     /gene="malF"
                     /locus_tag="J1138_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBA8453340.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7617..8807)
                     /gene="malE"
                     /locus_tag="J1138_001525"
     CDS             complement(7617..8807)
                     /gene="malE"
                     /locus_tag="J1138_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBA8453341.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            9172..10287
                     /gene="malK"
                     /locus_tag="J1138_001526"
     CDS             9172..10287
                     /gene="malK"
                     /locus_tag="J1138_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBA8453342.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10359..11699
                     /gene="lamB"
                     /locus_tag="J1138_001527"
     CDS             10359..11699
                     /gene="lamB"
                     /locus_tag="J1138_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBA8453343.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11931..12851
                     /gene="malM"
                     /locus_tag="J1138_001528"
     CDS             11931..12851
                     /gene="malM"
                     /locus_tag="J1138_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBA8453344.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13030..13527
                     /gene="ubiC"
                     /locus_tag="J1138_001529"
     CDS             13030..13527
                     /gene="ubiC"
                     /locus_tag="J1138_001529"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBA8453345.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13540..14412
                     /gene="ubiA"
                     /locus_tag="J1138_001530"
     CDS             13540..14412
                     /gene="ubiA"
                     /locus_tag="J1138_001530"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBA8453346.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14567..16990)
                     /gene="plsB"
                     /locus_tag="J1138_001531"
     CDS             complement(14567..16990)
                     /gene="plsB"
                     /locus_tag="J1138_001531"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBA8453347.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17161..17529
                     /locus_tag="J1138_001532"
     CDS             17161..17529
                     /locus_tag="J1138_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBA8453348.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17639..18247
                     /gene="lexA"
                     /locus_tag="J1138_001533"
     CDS             17639..18247
                     /gene="lexA"
                     /locus_tag="J1138_001533"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBA8453349.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18266..19645
                     /gene="dinF"
                     /locus_tag="J1138_001534"
     CDS             18266..19645
                     /gene="dinF"
                     /locus_tag="J1138_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBA8453350.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNDTWFVAT"
     gene            19761..19970
                     /locus_tag="J1138_001535"
     CDS             19761..19970
                     /locus_tag="J1138_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBA8453351.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(20012..20527)
                     /gene="zur"
                     /locus_tag="J1138_001536"
     CDS             complement(20012..20527)
                     /gene="zur"
                     /locus_tag="J1138_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBA8453352.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20683..21678
                     /gene="dusA"
                     /locus_tag="J1138_001537"
     CDS             20683..21678
                     /gene="dusA"
                     /locus_tag="J1138_001537"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBA8453353.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21812..22054
                     /gene="pspG"
                     /locus_tag="J1138_001538"
     CDS             21812..22054
                     /gene="pspG"
                     /locus_tag="J1138_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBA8453354.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(22219..23202)
                     /locus_tag="J1138_001539"
     CDS             complement(22219..23202)
                     /locus_tag="J1138_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBA8453355.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            23285..24700
                     /locus_tag="J1138_001540"
     CDS             23285..24700
                     /locus_tag="J1138_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HBA8453356.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24753..25832
                     /gene="alr"
                     /locus_tag="J1138_001541"
     CDS             24753..25832
                     /gene="alr"
                     /locus_tag="J1138_001541"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBA8453357.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLNEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            26085..27278
                     /gene="tyrB"
                     /locus_tag="J1138_001542"
     CDS             26085..27278
                     /gene="tyrB"
                     /locus_tag="J1138_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBA8453358.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDLRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYNEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLRTAQVDRLREEFGVYLIASGRMCVAGLNAQNVHRVAKA
                     FAAVM"
     gene            27460..30204
                     /locus_tag="J1138_001543"
                     /pseudo
     CDS             27460..30204
                     /locus_tag="J1138_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
     gene            30237..31391
                     /gene="odhB"
                     /locus_tag="J1138_001544"
     CDS             30237..31391
                     /gene="odhB"
                     /locus_tag="J1138_001544"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBA8453359.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL"
     gene            31419..32822
                     /gene="lpdA"
                     /locus_tag="J1138_001545"
     CDS             31419..32822
                     /gene="lpdA"
                     /locus_tag="J1138_001545"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBA8453360.1"
                     /translation="MWQLWGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTC
                     LNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRLTMGIG
                     LLFKKNKVKYLCGLATLEHARDEIWQLRVNDQQIRARNVVIATGSQPRQLPGVTIDNQ
                     QILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPALEARL
                     SNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAIGRVPR
                     LSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGVVVADQ
                     IAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGRALALG
                     QEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHAHPTLS
                     EVIHEAAMALNNKALHG"
     gene            32844..34013
                     /gene="sucC"
                     /locus_tag="J1138_001546"
     CDS             32844..34013
                     /gene="sucC"
                     /locus_tag="J1138_001546"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBA8453361.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVNSLDDAAKRIIALLN"
     gene            34026..34898
                     /gene="sucD"
                     /locus_tag="J1138_001547"
     CDS             34026..34898
                     /gene="sucD"
                     /locus_tag="J1138_001547"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBA8453362.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKEAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRDEMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
BASE COUNT         8234 a         8778 c         9577 g         8440 t
ORIGIN      
        1 cttatatttg atattcattc caattgtcct aaaactccat cactaattcc ccgcgccgta
       61 attcgcatgc tttagttgtg tatactcgat cccgcccgaa atgttttggg taaatctcca
      121 ttcattcaat gaagggaaat tgttatgaaa aaagttctgt atggcatttt tgccatatct
      181 gcgcttgcgg cgacttctgc gtgggctgca cctgtacagg tgggcgaagc ggcaggatcg
      241 gcagcaacgt cggtttcggc gggtagttcc tccgcgacca gcgtcagcac cgtaagctcg
      301 gcggtgggtg tcgcgctcgc ggcaaccggt ggcggtgatg gttctaatac cgggaccacc
      361 acaaccacga ccaccagtac ccagtaataa agtatgtatc cccaaaataa ttcgagtcat
      421 cgcatctgaa gcttgaagta tgaaggggat taaccataac cacactccgg tgtggttatt
      481 ctgcccctct ggagaagagt cgtgaagcga cctgcactca ttcttatctg cctgctatta
      541 caggcctgtt cagccacgac taaagagctg ggcaattcac tgtgggacag tctgttcggt
      601 acgccaggcg tacagctgac ggacgatgat attcaaaata tgccctacgc cagccagtac
      661 atgcagctta atggcgggcc gcagttattt gtggtgctgg ccttcgctga agacggacaa
      721 caaaaatggg tcactcagga tcaggccacc cttgtcacac aacatggtcg tctggtgaag
      781 actttgcttg gcggcgacaa cctgattgaa gtgaataacc ttgccgccga cccgctgatt
      841 aaacccgcac aaatcattga tggtgcaacc tggacgcgca cgatgggctg gaccgagtac
      901 cagcaggtac gctacgccac cgcgcgctca gtcttcaaat ggaatggcac cgataccgtc
      961 aaagtcggca gcgatgaaac cccggttcgc gtgctggacg aagaagtctt cactgaccag
     1021 gcgcgctggc ataaccgcta ttggatcgac agcgaagggc aaattcgcca gtcggaacag
     1081 tatctcggcg cggattactt cccggtgaaa accaccctca tcaaggcggc aaaacaatga
     1141 ttaaacaaac tattgtcgcg ttgcttttga gtgcgggggc gtcatcagtc ttcgcggcgg
     1201 gaaccgtcaa ggtgttcagc aatggcagca gtgaggccaa aacgctgacg ggcgcagagc
     1261 atttaatcga tctggtaggc caaccgcggc tggcaaacag ctggtggccc ggagcggtga
     1321 ttagcgaaga gctggccacg gcggctgcat tgcgtcagca gcaggcgttg ctgacacggc
     1381 tggcagaact ggctgcagat tccagcgccg acgatgccgc cgcgattaac gccttacgcc
     1441 agcaaattca ggcgttgaag gtgacgggca ggcaaaaaat caatcttgat cctgatatcg
     1501 tccgcgttgc cgaacgcggt aacccgccgt tgcagggcaa ctacacgctg tgggtcggaa
     1561 cgccgccgtc cacggtcacg ttgttcgggc ttatcagtcg tcctggcaag cagtcattca
     1621 ctccaggtcg cgacgtggcg agctatctct ctgaccagat cctgctcagc ggtgcggatc
     1681 gcagctacgc gtgggtggtt tacccggacg gacgcacgca aaaagcgccg gtggcttact
     1741 ggaacaagcg tcacgtagag ccgatgcccg gcagcattat ttatgttggc ctcgcggact
     1801 ccgtctggag tgagacacct gatgccctta acgccgacat tcttcagact ctgacgcagc
     1861 ggatacccca ataatgaaaa aaagacatct gcttagctta ctggcgctgg gcattagcac
     1921 agcttgctac ggcgaaacat atcctgcgcc cattggtccg tcgcaatcgg atttcggtgg
     1981 cgtaggatta ttacaaacgc ccaccgcgcg catggcgcgg gaaggggagc tgagcctgaa
     2041 ctatcgcgat aacgatcagt accgttatta ctcagcttca gtgcaactct tcccgtggct
     2101 ggaaacaacg ctgcgctaca ccgacgtgcg cacccggcag tacagcagcg tcgaagcgtt
     2161 ctctggcgat caaacgtata aagataaagc cttcgatctc aaactgcgtt tgtgggaaga
     2221 gagttattgg ctgccgcaag tggcggttgg tgcgcgggat atcggcggta cggggctgtt
     2281 tgatgcggaa tatcttgttg ccagcaaagc ctgggggccg ttcgatttta cgctcggcct
     2341 gggatgggga tatctgggca ccagcggtaa tgtgaaaaat ccgctctgtt cagccagtga
     2401 taaatattgc tatcgcgata acagctacaa acaagcggga tctatcgacg gcagtcagat
     2461 gttccacggc ccggcctcac tgtttggcgg cgtggaatac cagacgccct ggcaaccgct
     2521 gcgcctgaaa ctggagtatg aaggtaataa ttatcagcag gattttgccg ggaagctgga
     2581 gcaaaaaagt aagtttaacg tcggggcgat ttatcgcgtt accgattggg ccgacgttaa
     2641 ccttagctat gaacgtggca acacctttat gtttggcgtc acgctgcgca ccaactttaa
     2701 cgatctgcgc ccgtcctaca acgataacgc ccgaccgaaa taccagccgc aaccgcagga
     2761 tgccattttg cagcactcgg tggtggcgaa tcagttaacg ctgttgaaat acaacgccgg
     2821 actggccgat ccgcagatcc aggcgaaagg cgatacgctg tatgttaccg gcgagcaggt
     2881 gaaatatcgt gattcgcgcg aagggatcat ccgtgctaat cggatcgtga tgaacgatct
     2941 gccggatggg atcaaaacga tccgcattac ggaaaatcgc cttaacatgc cgcaggtgac
     3001 gacggaaacc gatgtcgcca gcctgaaaaa tcatctcggc ggagagccgt tgggccacga
     3061 aacgacgctg gcgcaaaaac gcgtcgagcc agtggttccg aagtccaccg agcagggctg
     3121 gtatatcgac aaatcacgct ttgatttcca tatcgatccg gtgctgaacc agtcggtcgg
     3181 tggcccggaa aacttttaca tgtatcagct gggcgtgatg ggaacggcgg atttgtggct
     3241 gacggaccat ctgctgacca ccggcagcct gtttgctaac cttgccaaca actacgacaa
     3301 gtttaactac accaatccac cgcaggactc gcacttaccg cgcgtgcgta cccatgtgcg
     3361 cgagtatgtg cagaatgatg tctatgtgaa taacctgcaa gccaactact tccagcatct
     3421 gggcaacggc ttctacggtc aggtctacgg aggttatctc gaaaccatgt ttggcggcgt
     3481 gggggcagaa gtgttgtatc gcccgctgga tagcaactgg gcgtttggtc tggatgccaa
     3541 ctacgttaaa cagcgcgact ggcgtagcgc aaaagatatg atgaaattca ccgattacag
     3601 cgtgaaaacc ggacatctga ccgcgtactg gacgccatct ttcgcccagg acgtgttagt
     3661 gaaagcgagc gtcgggcagt atctggctgg ggataaaggc ggcacgctgg agatcgctaa
     3721 acgctttgac agcggcgtgg tggttggcgg ctatgccacg atcaccaacg tttcgaaaga
     3781 ggagtacggc gaaggggact tcaccaaagg cgtgtatgtc tctgtaccgt tggatctctt
     3841 ctcgtctggc ccgacacgca gccgtgcggc gattggctgg acgccgctga cgcgtgacgg
     3901 tggtcagcaa cttgggcgta agttccagtt gtatgatatg accagcgacc gtagcgtcaa
     3961 tttccgctaa gtcatgggaa aggtgccagt tttcgcactc actggcacct tcattcttaa
     4021 tggcctgtcg agccatcagg aacgcagttc acccccggtt tgcgatctgg tgtgtaaagg
     4081 cgaaaacatt ctgtcttcct gtccgtcgat atcggttgca ggtctggttg cctgagcgac
     4141 tgggccatca ttggactgga gacattgctt tgcggtaggt cggctttatc agcagtaccg
     4201 agcggaccag cataagcagg aagaacagag actgataaca tcaaagcagc aaaataaggc
     4261 ttcattttta ccacctttat caggttacgt ttcatttgtt ccagaggaac attgtcgatt
     4321 tttcgcgcat tgctggtggc tgggaatcac ctgaatgggt gatttttttg ggctgtgtgt
     4381 tttgccagat gcgacgttga tgggagatgc ccacttaatt gagctgaaca aaacatacac
     4441 aaaaaatata gatctccgtc acatttttgc gttatacagg aagctcgcca ctgtgaagga
     4501 ggtactgcta tgacgtcact ctctcgtccg cgcgtggagt ttatctccac cattttgcag
     4561 accgtactca atcttggcct gttgtgcctc ggcctgattt tggttgtctt cctcggcaaa
     4621 gaaacggtgc atctggctga tgtgctgttc gcgccagaac aaaccagtaa atatgagctg
     4681 gtagaagggc tggtggttta ttttctctat ttcgaattta tcgcgctgat tgtgaagtac
     4741 tttcagtccg gttttcactt cccgctacgt tactttgtct atattgggat caccgccatt
     4801 gtgcgcttga tcatcgtcga tcataaatcg ccactggatg tgttgatcta ctcggcagcg
     4861 atcctgttac tggtgatcac cctgtggctg tgtaattcga aacggctgaa gcgggagtaa
     4921 aaaaaggcgc tccgtggagc gccgaataac agtcacaagt tgggataacg taagttgagg
     4981 gtgcagcggc ataacattgg cagaacaaca tctttaacct ttcacaccac ctgccgtcag
     5041 gccgttgacc agccagcgtt gagccagcaa gaagacgatg gtgatcggta atgcagacat
     5101 cacggcagcg gcggcaaagt caccccacag gtagttttgc gggttgaggt attgctgcat
     5161 ccccacggcc agggtgtagc tgtttacgtc acgcagtaac agcgatgcga ccggaacttc
     5221 agtaatggca gcgataaacg acaggatgaa cacaaccgcc agaatcggta ctgacagcgg
     5281 caacaggaca aggcggaagg cctgccacgg tgtcgcacca tccagcgccg ccgcttcttc
     5341 cagcgaactg tcgatggttt cgaaataacc tttgatggtc cagacatgca gcgcaatccc
     5401 gcccagatac gcgaaaatta cgccgccgtg agtattcagg ccaatgaatg gaatgtactc
     5461 acccagacga tcaaacaacg catacaacgc gaccagtgaa agtactgccg ggaacatctg
     5521 gaaaatcagc attcctttca gcagcgtcgc tttgcctgga aagcgcatac gggcgaaagc
     5581 gtaggcgcag gtggtggaga gcgccacaat gccaatcgcg gaaatcccgg cgacctttac
     5641 cgagttccac agccacagca gtaccgggaa tggcggtggc gtaatgcgac catcagcctg
     5701 ttccacacta aaacctaacg ccagtttcca gtgatcccag gagatttgct ccgggatcag
     5761 actaccggtc gcgaagttcc cctgacgcag cgagatggcg acgaccatca gcagcgggaa
     5821 cataattgcc gcgataaaaa gtagcagtag caggtgagta ataaacaaac gtgctttttg
     5881 cgatttcggt tggaccattg ccatttttgt tatctccctt aatcaaactt cattcgcgtg
     5941 gctttcaggt tcactatcgc cagcgcgccc accagcagga agatcagcgt ggcaattgct
     6001 gctgccagac cgaagtcctg acccccgccg ccttcaaaag cgatacggta ggtgtagtta
     6061 acaagcaggt cggtataacc ggctggcgtg gtcgtgccaa gacgatccgg gccgccgttg
     6121 gttaacagtt gaatcagcac gaagttgtta aagttaaatg caaagctggc gatcatcagc
     6181 ggcgtcagcg gtttaatcag cagcggcagc gtaatcttaa agaagttctg gaatggacct
     6241 gcgccatcca ttgctgaggc ttcatacaaa tcgtccggaa tcgctttcag caagcccatg
     6301 cagaggatca tcatgtacgg ataacccagc caggtgttga cgataatcag catcgtgcgg
     6361 gcggtggtcg gatcgctgaa ccaggcgggc ttcacgccaa acagcgcgct caacatcatg
     6421 ttgatttcac cgaaactctg gttaaacaac cctttgaaaa tcaagattga aatgaacgat
     6481 ggcaccgcgt agggcagaat cagcaggacg cgatagaccg ctttgccgcg caacgcttcc
     6541 cactgcacca gacacgccag aaccatgccg accgccaccg ttaaaaagac agtgatcagc
     6601 gagaacacca cggtccagac gaaaatggcg aggaacggtt tctgaatgcc ttcgtcggta
     6661 aagacgcggg taaagttttt ccagccggtg gtcacggtgt aaccggggct tagcttttca
     6721 tccccccagt taccgtcggc ggtaatggat tggtaaaaac caatttggtt attcggccga
     6781 tatttcacgc cgctctggtt attcgtcaac gtgccgtcac cgtcgagtgt gtagagcggc
     6841 tgcgtgccag aaaactggcg cagggagctc atcatcactt tgttgccatc cggcagaatg
     6901 gcggtaatgt cactcagtgc ctgacgattc tgggtaatta cgcgcagatt cgcgcgttcg
     6961 ccttcgggct gggcggtcgt ttctttcagt tgcagttttt gctcgccgcc aaatttaaac
     7021 gcgtcggaga ggtaattttt gccggtttcg ccgtcgctga gcgccagttg ccactcatcg
     7081 cccgccggat aaagaccaaa attatagatt ttgcctgctt gccaggagcg atctagcaac
     7141 acttcctgcg cacgttcaaa agtcagctgg ttagtgctgc tgtagttggt gaaggcaatg
     7201 gcgatggtgc agaccagagg gaagaggacg aataatccca ttccagccat tcccgggtaa
     7261 acatagcgcc aggcgtaggc tttacgattg gcgaaaatat acagccccgc tgagctcaat
     7321 atcagcgtgg taatggcgaa caggtattcc ccttgtgcgt acattaaaac aacaaggtaa
     7381 cccaccagca ggccgagcag acctaacact gaccatttca gcgcgtcgct ttgccaccaa
     7441 tgtttctttt taatgacatc catggggttc ttcctcattc caggacggat aaggctttca
     7501 taccttatcc gacaacaact gcctgatgcg acgctgacgc gtcttatcag gcctacatac
     7561 gtttcaattt cgtaggccgg ataaggcgtt tacgccgcat ccggcatttc acagcattac
     7621 ttggtgatac gagtctgtgc atctttcagc gcagcatcca cagtttgacg accgctggcg
     7681 gcgttgatca ccgcagtacg cacggcatac cagaaagcgg acatctgcgg gatgttcggc
     7741 ataatttcac ctttctgagc gttatccatg gtggcggcaa tacgtggatc tttcgcaagt
     7801 tcatcctgat aggacttcag cgctactgca cccagcggtt tgtctttatt gaccgcttcc
     7861 agaccttcgt cagtcagcag atagttttcg aggaactctt tcgccagctc tttgttcgga
     7921 ctggcggcgt taatacctgc gctcagcacg ccaacgaacg gtttagatgg ttgacctttg
     7981 aaggtcggca gtaccgttac accataattc actttactgg tgtcgatgtt ggaccatgcc
     8041 cacgggccgt tgatagtcat cgctgtttcg cctttattaa aggcagcttc tgcgatggag
     8101 taatcggtgt ctgcattcat gtgtttgttt ttaatcagat caaccaggaa ggtcagaccc
     8161 gctttcgcgc cagcgttatc cacgcctacg tctttaatgt cgtacttgcc gttttcatac
     8221 ttgaacgcat aacccccgtc agccgcaatt agcggccagg tgaagtatgg ttcttgcagg
     8281 ttgaacatca gcgcgctctt acctttcgct ttcagttctt tatccagcgc cgggatctct
     8341 tcccaggttt ttggcgggtt cggtagcaga tctttgttat aaatcagtga taacgcttcg
     8401 acagcgatcg ggtaagcaat cagcttgccg ttgtaacgta cggcatccca ggtaaacgga
     8461 tacagcttgt cctggaacgc tttgtccggg gtgatttcag ccaacaggcc agattgagcg
     8521 taaccaccaa agcggtcgtg cgcccagaag ataatgtcag ggccatcgcc agttgccgca
     8581 acctgaggga atttctcttc cagtttatcc ggatgctcaa cggtgacttt aattccggta
     8641 tctttctcga atttcttacc gacttcagcg agaccgttat agcctttatc gccgttaatc
     8701 cagattacca gtttaccttc ttcgattttg gcgagagccg aggcggaaaa catcatcgtc
     8761 gttaatgcgg ataatgcgag gatgcgtgca cctgttttta ttttcataat ctatggtcct
     8821 tgttggtgaa gtgctcgtga aaacacctaa acggactcta gtttctttat acggcaacct
     8881 ctttccatcc tccttgcccc tacgccccat cgttgctttg tgtgatctct gttacagaat
     8941 tggcggtaat gtggagatgc gcacataaaa tcgccatgat ttttgcaagc aacatcacga
     9001 aattccttac atgacctcgg tttagttcac agaagccgcg ttctcatcct cccgcctcct
     9061 cccccataaa aaagccaggg ggtggaggat ttaagccatc tcctgatgac gcatagtcag
     9121 cccatcatga atgttgctgt cgatgacagg ttgttacaaa gggagaaggg catggcgagc
     9181 gtacagctgc aaaatgtaac gaaagcctgg ggcgaggtcg tggtatcgaa agatatcaat
     9241 ctcgatatcc atgaaggtga attcgtggtg tttgtcggac cgtctggctg cggtaaatcg
     9301 actttactgc gcatgattgc cgggcttgag acgatcacca gcggcgacct gttcatcggt
     9361 gagaaacgga tgaatgacac tccgcctgca gaacgcggcg ttggtatggt gtttcagtct
     9421 tacgcgctct atccccacct gtcagtagca gaaaacatgt catttggcct gaaactggct
     9481 ggcgcgaaaa aagaagtgat taaccagcga gttaaccagg ttgcggaagt gctgcaactg
     9541 gcgcatttgc tggatcgcaa accgaaagcg ctctccggtg gtcagcgtca gcgtgtggcg
     9601 attggccgta cgctggtggc cgagccaagc gtatttttgc tcgatgaacc gctctccaac
     9661 ctcgatgctg cactgcgtgt gcaaatgcgt atcgaaatct cccgtctgca taaacgcctg
     9721 ggccgcacaa tgatttacgt cacccacgat caggtcgaag cgatgacgct ggccgacaaa
     9781 atcgtggtgc tggacgctgg tcgcgtggcg caggttggga aaccgctgga gctgtatcac
     9841 tatccggcag accgttttgt cgccggattt atcggttcgc caaagatgaa cttcctgcca
     9901 gtaaaagtga ccgccaccgc aatcgatcaa gtgcaggtgg agctgccgat gccaaatcgt
     9961 cagcaagtct ggctacctgt tgaaagccgt gatgtccagg tgggagccaa tatgtcgctg
    10021 ggtattcgcc cggaacatct actgccgagt gatatcgctg acgtcatcct tgagggtgaa
    10081 gttcaggtcg tcgagcaact cggcaacgaa acccaaatcc atatccagat cccttccatt
    10141 cgtcaaaacc tggtgtaccg ccagaacgac gtggtgttgg tagaagaagg tgccacattc
    10201 gctatcggcc tgccgccaga gcgttgccat ctgttccgtg aggatggcac tgcatgtcgt
    10261 cgactgcata aggagccggg cgtttaagca ccccacaaaa cacacaaagc ctgtcacagg
    10321 tgatgtgaaa aaagaaaagc aatgactcag gagatagaat gatgattact ctgcgcaaac
    10381 ttcctctggc ggttgccgtc gcagcgggcg taatgtctgc tcaggcaatg gctgttgatt
    10441 tccacggcta tgcacgttcc ggtattggct ggacaggtag cggcggcgaa caacagtgtt
    10501 tccagactac cggtgctcaa agtaaatacc gtcttggcaa cgaatgtgaa acttatgctg
    10561 aattaaaatt gggtcaggaa gtgtggaaag agggcgataa gagcttctat ttcgacacta
    10621 acgtggccta ttccgtcgcg caacagaatg actgggaagc taccgatccg gccttccgtg
    10681 aagcaaacgt gcagggtaaa aacctgatcg aatggctgcc aggctccacc atctgggcag
    10741 gtaagcgctt ctaccaacgt catgacgttc atatgatcga cttctactac tgggatattt
    10801 ctggtcctgg tgccggtctg gaaaacatcg atgttggctt cggtaaactc tctctggcag
    10861 caacccgctc ctctgaagcg ggtggttctt cctctttcgc cagcaacaat atttatgact
    10921 ataccaacga aaccgcgaac gacgttttcg atgtgcgttt agcgcagatg gaaatcaacc
    10981 cgggcggcac attagaactg ggtgtcgact acggtcgtgc caacctgcgt gataactatc
    11041 gtctggttga tggcgcatcg aaagacggct ggttattcac tgctgaacat actcagagtg
    11101 tcctgaaggg ttttaacaag tttgttgttc agtacgctac tgactcgatg acctcgcagg
    11161 gtaaaggtct gtcgcagggt tctggcgtcg cgtttgataa cgaaaaattt gcctacaata
    11221 tcaacaacaa cggtcacatg ctgcgtatcc tcgaccacgg tgcgatctcc atgggcgaca
    11281 actgggacat gatgtacgtg ggtatgtacc aggatatcaa ctgggataac gacaacggca
    11341 ccaagtggtg gaccgtcggt attcgcccga tgtacaagtg gacgccaatc atgagcaccg
    11401 tgatggaaat cggctacgac aacgtcgaat cccagcgcac cggcgacaag aacaatcagt
    11461 acaaaattac ccttgcacaa caatggcagg ctggcgacag catctggtca cgcccggcta
    11521 ttcgtgtctt cgcaacctac gccaagtggg atgagaaatg gggttatgac tacaccggta
    11581 gctcttcaac caacccatac tacggcaagg ctgtgtcggc tgatttcaac ggcggtagct
    11641 tcggtcgtgg cgacagcgac gagtggacct tcggtgccca gatggaaatc tggtggtaat
    11701 agcaaaacct gggccggata aggtgcttgc gtcgcatccg gcaaccaatg cctgatgcga
    11761 cgctgttgcg tcttatcagg cctacaactg ctgccgaaag taggccggat aaggcgttta
    11821 cgccgcatcc ggcataaaaa cagctttaag gggcgaaagc ccctctgatt atcgggttta
    11881 gcgcgctatt gcctggctac cgctgagctc cagattttga ggtgaaaaca atgaaaatga
    11941 ataaaagtct catcgctctc tgtttatcag cagggttgct ggcaagcgca cctggaatca
    12001 gccttgccga tgttaactac gtaccgcaaa acaccagcga cgcgccagcc attccatctg
    12061 ccgcgctgca acaactcacc tggacaccgg tcgatcaatc taaaacccag actactcaac
    12121 tgtcgaccgg cggccaacaa ctgaatgtcc ctggcatcag tggtccggtt gctgcgtaca
    12181 gcgtcccggc aaacattggt gaactgacac tgacgctgac cagcgaagtg aacaaacaaa
    12241 ccagcgtatt tgcgccgaat gtgctgattc ttgatcagaa catgacccca tcagccttct
    12301 tccccagcag ttatttcacc taccaggaac ccggcgtgat gagtgcagat cggctggaag
    12361 gcgttatgcg cctgacaccg gcgttggggc agcaaaaact ttatgttctg gtctttacca
    12421 cggaaaaaga tctccagcag acgactcaac tgctcgaccc ggctaaagcc tatgccaaag
    12481 gcgtcggtaa ctcgatcccg gatattcccg atccggttgc tcgtcatacc accgatggct
    12541 tactgaaact gaaagtgaaa acgaactcca gctccagcgt gttggtagga ccactatttg
    12601 gttcttccgc tccagctccg gttacggtag gcaacacggc ggcaccagct gtggctgcac
    12661 ccgctccggc accggtgaag aaaagcgagc cgatgctcaa cgacacggaa agttatttta
    12721 ataccgcgat caaaaacgct gtcgcgaaag gtgatgttga taaggcgtta aaactgcttg
    12781 atgaagctga acgcctgggg tcgacatctg cccgttccac ctttatcagc agtgtaaaag
    12841 gcaaggggta attacgcccc acagtgctga ttttgcaaca actggtgcgt ctcctggcgc
    12901 accttttttt ctcctcctgt actattgttg cgtttttgtt gcgcattagt tcacttaatt
    12961 tgctttacat ctcccgtaaa tatttttctg cgatacaatg cctttacgtt atgtaacgga
    13021 gagttcggca tgtcacaccc cgcgttaacg caactgcgtg cgctgcgcta ttttacagag
    13081 atccctgccc tggagccgca actgctcgac tggctgttgc tggaagattc catgacaaaa
    13141 cgttttgaac agcagggaaa aacggtgagc gtgacgatga tccgcgaagg gtttgtcgag
    13201 cagaatgaaa tccccgaaga actgccactg ctgccgaaag agtctcgtta ctggttacgt
    13261 gaaattttgt tatgtgccga tggtgaaccg tggcttgccg gtcgtaccgt cgttcctgtg
    13321 tcaacgttaa gcgggccgga gctggcgtta caaaaattag gtaaaacgcc gttaggacgc
    13381 tatctgttca catcatcgac attaacccgg gactttattg agataggccg tgatgccgga
    13441 ctgtgggggc gacgttcccg cctgcgatta agcggtaaac cgctgttgct aacagaactg
    13501 tttttaccgg cgtcaccgtt gtactaagag gaaaaaaata tggagtggag tctgacgcag
    13561 aataagctgc tggcgtttca tcgcttaatg cgtacggata agccaattgg cgcgttactg
    13621 ctgctctggc caacattatg ggcgttgtgg gtggcgacac cgggcgttcc ccagctctgg
    13681 atcctggcgg tgtttgtcgc gggcgtctgg ctgatgcgcg ctgctggttg cgtggtgaat
    13741 gattatgctg accgcaagtt tgatggtcat gttaagcgca cggcgaaccg accacttccc
    13801 agcggcgcgg taacggagaa agaggcgcgc gcgctgtttg tagtgctggt actgatttca
    13861 tttttactgg tgctgacgct gaatacgatg actattctgt tgtcgattgc cgcgctggcg
    13921 ctggcgtggg tatacccgtt tatgaagcgg tatacccatc taccgcaagt ggtgctgggc
    13981 gcggcgtttg gctggtcgat tccaatggct tttgccgctg tgagtgagtc ggtgccattg
    14041 agttgctggt taatgttcct cgccaatatt ctctgggcgg tggcttacga cacgcagtat
    14101 gcgatggttg accgcgatga tgatgtgaag attggcatta aatccaccgc aatcctgttc
    14161 ggccaatacg ataaattgat tattggtatt ttgcagattg gcgtactagc gctgatggcg
    14221 atcatcggtg agttaaatgg cttaggctgg ggatattact ggtcaattct ggtagctggc
    14281 gcgctgtttg tttatcaaca aaaactgatt gccaaccgcg agcgtgaagc ctgctttaaa
    14341 gcatttatga ataataacta tgttggtctg gtactgtttt tagggctggc aatgagttac
    14401 tggcatttct gatgatgtaa aaaagccgga tgatcatccg gctttcttct gggttgcctg
    14461 atgcgctgcg cttctcaggc ctacacaaca tatcgcaatt tattgaattt gcagatcatg
    14521 gtaggccgaa taaggcgttt gcgtcgcatc cggcaattct cactgattac ccttcgccct
    14581 gcgtcgcact ctcaatcgtc aaacgcacgt ctgatgtaat caactccgcc agcaactgat
    14641 acaccttcat cgtttctgcc ggttcggcgt cgccgctatc gctgatatac ccttcatcac
    14701 gcagtgtcag caccagagaa ctgaacaccg ccttgtcgaa gaactccggc gcgttgatac
    14761 cgtgcagcac ggagagacgt tgcgcgacgg tgcggctctc tttctccagc gtaccgcggt
    14821 tgatcgacgg gttggcactc aacagccaga aggtgatggc ataacgttgc agcgtttcgc
    14881 gcgcgcctgc ggccagcagc tgtagcgtgc gagaatgcgc cgggttgata tgcaactcat
    14941 catcttgcag ggtaatcagc ccctgacgtt gcatctcatt tgccagcgca tcaataacgt
    15001 ccggtaactc gtcgcgatcc cagcgcagga acagctccgc tttcagcatt gggtaaagca
    15061 cattgacgtg ctccatcaat acgtcgcggg agatgtggcg atgctgggtg acaattgccg
    15121 ccatcagcga aggcagcacc aacatatgtg caatgttgtt gcgatagtag gtcatcagca
    15181 ccgcttgctc gcgcggcaga atgatgatgt cgccgatagt gtctttctca acttcaaact
    15241 tgttcatttg cagcgcgtga tcgataagct cgctggcgct ggctgaagga acggtagagt
    15301 ccgtggagta aggtacgttg cgcatcagat ccaggtagca gttgagttgc tcggttaact
    15361 gctcgcgggt gagtgagcgc tgacgtgacg ccagaagcgc agtacagcac aggttcatgg
    15421 cgtttgccgc gcctgcgttg ttaatgcgta ccatcagatc ggcagcaata ttattgaccg
    15481 tcggcgttaa ccaggccgga cgcaccgctt cgatgggatc gatagattca cgccagtctg
    15541 gtacgtgctg gttaaggtag gtcatcaacg gcattggttc accgaagttg acgtaaccct
    15601 gaccgagatt acgcagcttg cttaaaccac gcagcatctg cggcaggctc tctttctctt
    15661 tcgtcgcacc gcgcagttct ttggcgtaag tacccacttc catgacgtgc tcataaccga
    15721 tatagatcgg aatcagcgta atcggacgag tgccgccacg cagcatcgcc tgaatggtca
    15781 tcgacagcgt accggttttc ggatccagca aacgccccgt acgggaacga ccgccttcca
    15841 cgaaatactc gacggaataa ccacggctga acagttcgcc gagatactca cggaaaacgg
    15901 tggaataaag tttattgcct ttaaacgtac ggcgaataaa gaacgccccc agacggcgga
    15961 aaatcggccc ggccggccag aagttcaggt tgatcccggc ggcgatatgc ggcggcacca
    16021 gcccctggtg atacagcacg taagaaagca gcaggtagtc catgtgactg cggtggcaag
    16081 gcacatacac tagctcatgg ccgtcgtggg ccagctggcg aacgcgctcg gcgttatgga
    16141 cgttgatacc ctggtaaagt cggttccagg tgaagcccag aatgcggtca gtcaggcgga
    16201 tcatctcgta agagaaattc gccgcaatct cttccatcag cgcaatcgcg ttctgctgcg
    16261 ctttttcatg ggagattttt ttgctgcgtg cttcatcttc taccgctttc gcaatggcgc
    16321 gggaggcgag cagcttatta aacagatcct gacgagcagg cagacgcgga cccacggcag
    16381 ccagacgttg acgggcaaag tgcatacgtg ctacgcgcgc cagtttctga gcgatagttt
    16441 tatccgtgcc gtgttcatcc gccatacggc gcagcgaaac tgacggcgag aaacgcacaa
    16501 aactgtcgcg accgagccac agtacagcga aaaatttctg tacgccgtta agcatacgca
    16561 gcggcgggtt cacttcgcct ttttcacgcc ccggcgcgcg accaaacatc accgatactg
    16621 gcaccatctg cacatccaga tttgggttgc tgcggtgcaa atcgagatag tcgtggaaca
    16681 gcttaataga ctcttctttc ggcgtgtaat aggtgaacac acgcggcccg ccgtgaatga
    16741 acacatagcg cggcagtagc gtgccgtcga tttccagcgg ctctaacggg tcaggcaggt
    16801 catgtgccag acactgggcg cgcaacgtca gcaagtcagc tttcgagttg tacggtaaaa
    16861 cgtacataat tggacgagag gtatccagcc ccagttccgg ggcaggatcc gccggaatag
    16921 acttgctttt taccaggatg cttaatggta aattcagtaa tttgtagtaa attcgtggcc
    16981 agccggacat aaacgatgta aagcctctgg ttaataatgc aaatgcgcgg caaggatagc
    17041 agaaagtcat gggaaattct gtggtatccg ctcatgtttc gcgcggcgtt acgcaaaccc
    17101 gaatcatcgg atttaacggt acactgatat tgacgctcat aatgtaaaaa ggttctttca
    17161 atggccaata ataccactgg attcatccga attattaaag ctgctggcta ttcctggaaa
    17221 ggtttacgcg ctgcatggat caacgaagcg gcattccgtc aggaaggcgt agcggtattg
    17281 ttagcggtgg ttatcgcctg ctggctggat gtggacgcga ttacccgcgt gctgcttatc
    17341 agctccgtga tgctggtgat gattgtggaa attctcaata gcgccatcga agctgtggtt
    17401 gaccgaattg gctctgaata ccatgagctt tccggacgcg ctaaagatat gggatccgcc
    17461 gcagtgctga ttgccattat cgtcgccgtg attacctggt gcatcctgtt atggtcgcat
    17521 tttggataac ccttccagat ttcgataaat ctctggttta ttgtgcagtt tatggttcca
    17581 aaatcgcctt ttgctgtata tactcacagc ataactgtat atacacccag ggggcggaat
    17641 gaaagcgtta acggccaggc aacaagaggt gtttgatctc atccgtgatc acatcagcca
    17701 gacaggtatg ccgccgacgc gtgcggaaat cgcgcagcgt ttggggttcc gttccccaaa
    17761 cgcggctgaa gaacatctga aggcgctggc acgcaaaggc gttattgaaa ttgtttccgg
    17821 cgcatcacgc gggattcgtt tattgcagga agaggaagaa gggttgccgc tggtaggtcg
    17881 tgtggctgcc ggtgaaccgc ttctggcgca acagcatatt gaaggtcatt atcaggtcga
    17941 tccttccttg ttcaagccga atgctgattt cctgctgcgc gtcagcggga tgtcgatgaa
    18001 agatatcggc attatggatg gcgatttgct ggcagtgcat aaaactcagg atgtacgtaa
    18061 cggtcaggtc gttgtcgcac gtattgatga cgaagttacc gttaaacgcc tgaaaaaaca
    18121 gggcaataaa gtcgaactgt tgccagaaaa cagcgagttt aaaccaattg tcgttgacct
    18181 tcgtcagcag agcttcacca ttgaagggct ggcggttggg gttattcgca acggcgactg
    18241 gctgtaacat atctctgaga ccgcgatgcc gcctggcgtc gcggtttgtt tttcatctct
    18301 cttcatcagg cttgtctgca tggcattcct cacttcatct gataaagcac tctggcatct
    18361 cgccttaccc atgattttct ccaatatcac cgttccgttg ctgggactgg tcgatacggc
    18421 ggtaattggt catcttgata gtccggttta tttaggcggc gtggcggttg gtgcaacggc
    18481 gaccagcttt ctctttatgt tgttgctgtt tttacgcatg agcaccaccg ggctgactgc
    18541 acaggcttat ggtgccaaaa atcctcaggc attagcccgt gcgctggtgc aaccgttgct
    18601 gttggcgttg ggggccgggg cgttaattgc gctgctgcgt acgccgatta tcgatctggc
    18661 gctgcatatt gttggtggta gtgaagcagt cctggaacag gcgcgacgct ttcttgaaat
    18721 ccgctggtta agcgcaccgg cgtcgctggc gaatctggta ttacttggtt ggttactcgg
    18781 cgtgcaatat gcccgtgcgc cagtaatttt gttagtggtc ggcaatatcc tcaacattgt
    18841 gctggatgtc tggctggtga tggggctgca tatgaacgtg cagggcgcgg ctctggcaac
    18901 ggttattgcg gaatatgcaa cattgctgat tggtctgcta atggtgcgta aaatcctcaa
    18961 actacgcgga atctccggcg aaatgctgaa aacagcctgg cgaggaaact tccgtcactt
    19021 gctggcgctt aaccgcgata tcatgctgcg ctcgctgttg ctgcaactct gtttcggcgc
    19081 gatcactgta cttggcgcgc gactggggag tgacattatc gctgttaacg cggttctgat
    19141 gacgctactc acctttaccg cctatgcgtt ggatggtttt gcctacgcgg ttgaagcaca
    19201 ttccggtcag gcatacggtg cgcgcgacgg tagtcaattg ctggatgtct ggcgggcagc
    19261 gtgccgccag tcggggatcg tagcgttact gttttcggtg gtttatttgc tggcagggga
    19321 acacatcatt gcgttgctga cgtcgttaac ccagattcag cagctggctg accgctatct
    19381 tatctggcag gtgattttgc cgttggtcgg cgtctggtgt tatctgctgg acggcatgtt
    19441 tataggcgca acgcgtgccg ccgaaatgcg taacagtatg gcggtggccg ccgcaggttt
    19501 tgcgctgacg ctccttacgc tgccgtggct ggggaatcat ggtttgtggc tggcgttaac
    19561 cgtctttctg gcgttacgcg ggctttctct ggcggctatc tggcggcgtc actggcgtaa
    19621 cgatacctgg tttgtcgcaa cgtgacggtt aaaaattctg aataaataat cctaagccaa
    19681 attgctgact acacttaatc tcacgttcag aagaaaagtg aacgtactct cattcacaac
    19741 ctaacgatga ggtcttgatt atgaataaag atgaagccgg cggtaactgg aaacagttta
    19801 aaggtaaagt gaaagagcaa tggggcaaac tgaccgatga tgatatgacg atcattgaag
    19861 gaaaacgtga tcagttagtg ggtaaaatcc aggaacgcta cggttatcag aaagatcagg
    19921 cagaaaaaga ggttgatagt tgggaaaaac gccatgatta ccgctggtaa ttaatccctc
    19981 ctgcccgaca tgtacaagga tgtacaccct tttagcgagg tttctttttc acctgaatag
    20041 agtggtcatg atggcactgt tcgggatgac gacacgcttc tacttctaca catgctgaac
    20101 ataatccatg tgcttcaata acattatgcc gcagggcaaa acccattttt gccgccagcg
    20161 tatgcatgat gtcttccacg ccttcagcac attcttcttt caccgcgccg cagcgatcgc
    20221 aaataaacat ggctgaagta tgggtgggct gatcgaacag atgacagagc acataactgt
    20281 tggtggattc caccttatgc acaaaacctt gttcaagcag aaactccagc gcgcgataaa
    20341 ccgttggcgg cttggcttgc ggttcagctt cgcgcagcaa atcaagcaga tcataagcgc
    20401 tgatagcgcc atcctgcaga ctcatcaggc gcaacacttc gagacgctgt ggggtcaggc
    20461 gcacattacg ctgtgcgcag attttttcag cctgcgctaa taactcctgc gttgtggtct
    20521 tttccattgg gcacctcgac agtggggtta agaatgtcct tactttacca tagttctcac
    20581 ttaaacgccg agaacagggt gtcatggctg tcacattgcc tgatcgcata ttcgttatgc
    20641 tatagtagcg cccccaattt accagatgct taaaaagtcg ccatgccagc agaatcacac
    20701 actgtttatc ccgcttaccg tttctcgatt gccccgatgc ttgactggac ggacagacat
    20761 tgccgttatt tcctgcgtct gctttcccgc aatacgttgc tgtataccga aatggtgacc
    20821 acaggggcga ttattcacgg taaaggtgat tacctggcgt acagtgaaga agaacatccg
    20881 gtagcgttgc aactcggggg tagcgatccg gcggcgctgg cacagtgtgc gaagctggca
    20941 gaagcgcgcg gatatgatga gatcaacctg aatgtcggct gcccgtctga ccgggtgcag
    21001 aacggcatgt ttggtgcgtg tctgatgggt aatgcgcagc tggttgccga ctgcgtgaaa
    21061 gcgatgcgcg atgtggtgtc gattccggtg acggtgaaaa cgcgtattgg catcgatgat
    21121 caggacagct atgaatttct ctgcgatttc atcaacaccg tttccggcaa aggtgagtgt
    21181 gagatgttca tcatccacgc acgtaaagcc tggctttctg ggttaagtcc gaaagaaaac
    21241 cgtgagatcc cgccgctcga ttatccgcgt gtgtatcaac tgaagcgtga ctttccgcat
    21301 ctgacgatgt cgattaacgg tggtatcaag tcgctggaag aagctaaagc gcatttgcaa
    21361 catatggatg gcgtgatggt cgggcgcgag gcgtatcaga atccgggtat tctggcggcg
    21421 gtagaccgag agatttttgg ttcctcggat actgatgctg atccggtggc ggtagtgcgc
    21481 gccatgtatc cgtacattga gcgtgaactc agccagggta cgtatctcgg ccatattacc
    21541 cggcatatgc tgggcttgtt ccagggtatt cctggcgcgc ggcagtggcg gcgttattta
    21601 agtgaaaatg cccataaagc gggtgcagac attaatgtgc tggaacacgc gctcaaactg
    21661 gtggcggata agcgttaact tttcaccaaa aagtagtcaa attcaccacg ccctgcgccc
    21721 cgtcgcgggg cgttttgctg ttaaatcaat agattatttt tggcatgatt cttgtaatgc
    21781 cagcaagaga tttcatattt gggagagcat catgctggaa ctactttttg taattggctt
    21841 ttttgtcatg ctgatggtca ccggtgtttc gttgctgggt attatcgccg cgctgattgt
    21901 ggcgacggcc tttatgttcc tcggcggtat gctggcattg atgattaagt tgctgccgtg
    21961 gctgctgttg gcggttgcgg tggtgtgggt gatcagggcg attaaagcac caaaagtgcc
    22021 gaaatatcag cgttatgacc gctggcgtta ctaagggatt gtgcggatga tcacaaccta
    22081 aggttttatc cttagaacaa aataggaatt gataatcaaa tctgtcacta ttgcgcgtca
    22141 aacaaattca tcgagctgta ccctacatac agccgaacta taaaagaaag ggcttcccag
    22201 gtggaagccc aatttctttt atggaatcaa caggctggaa ccctgcgtcg cccggctttc
    22261 cagaatctca tgcgcacgcc gcgcatcctt cagcggatat ttctgctgct cggcgacatc
    22321 gaccttaatc acaccgctgg caatcaaaga gaacagttca ttactggcct cggttaattc
    22381 ctcccgcgtg gtgatatagc cttgcaggga agggcgtgtc acatacaacg agcctttctg
    22441 attgagaatg cctaagttca caccggtaac cgcacctgat gagttgccaa agctcaccat
    22501 taagccgcgg cgttgcaggc aatccagcga ccgttcccag gtgtctctgc ccactgaatc
    22561 gtacaccacg cgcactttct taccaccggt gatctctttc aatcgctcga ccagattctc
    22621 ttcacgatag ttaataacct gccacgcgcc cgcttttagc gcactctgcg ctttttgcgc
    22681 ggttcctacg gtgccgataa gtttcgcgcc cagggctttt gcccactggc aggcaatcaa
    22741 gccaacgccg ccagccgctg cgtggaacag gaattgttca tcgggtttaa tttcataggt
    22801 tttgcgcagc agataataaa ccgttaagcc tttcaggaag gatgccgcag cttgctcaaa
    22861 agaaattgcc gcaggcagaa tcgccgcttt atccgcatta atgttatgca cagagctgta
    22921 agcgcctaat gccgattgtg catagactac gcgatcgcct gccttaatat gctttacacc
    22981 actgccgact ttactcacga tgcctgccgc ttcagtacct aatccgctgg gtaacgatgg
    23041 tggcgggtaa aggccgctgc ggatatatgt gtcgataaaa ttgatgccga tggctttatt
    23101 ttcgacctgg atttcattct ccgccggatc ggcaggagtg aactctacgg cttgaagtac
    23161 ttccgggcca ccgtgcttgt gaaattcaat tcgtgttgcc atgtgtcctc cagaacgtat
    23221 cgtcagggtc tgcttcatat ggtaaagttt cgacccattc tttatctcgg taactccatt
    23281 cactatggca ggaaataaac ccttcaacaa acagcaggct gaaccccgcg aacgcgatcc
    23341 acaagttgcc gggctaaaag tgcctccgca ctcgatcgaa gcggagcagt cggtgttggg
    23401 cggtttaatg ctggataacg aacgctggga tgatgtagcc gagcgtgtgg tggcagacga
    23461 tttttacacc cgcccacacc gtcatatctt tactgaaatg gcgcgtttgc aggaaagcgg
    23521 tagtcctatc gatctgatta cccttgcgga atcgctggaa cgccaggggc aactcgatag
    23581 cgtcggtggt tttgcttatc tggcagagct gtcaaaaaat acgccaagtg cggcgaacat
    23641 cagtgcatat gcggatatcg tgcgcgaacg tgccgttgtt cgtgagatga tctccgttgc
    23701 taacgagatt gccgaagctg gttttgatcc acaagggcgt accagtgaag atctgctcga
    23761 ccttgctgaa tcccgcgtct ttaaaattgc cgaaagtcgt gcaaacaaag acgaagggcc
    23821 gaagaacatc gccgatgtgc tcgacgcaac cgtggcgcgt attgagcagt tgtttcagca
    23881 gccacacgat ggcgttaccg gggtaaatac cggttatgac gatctcaaca aaaaaaccgc
    23941 tggcttgcag ccgtcggatt tgatcatcgt tgccgcgcgt ccgtcgatgg gtaaaacgac
    24001 atttgcgatg aacctcgtcg aaaacgcggc gatgttgcag gataaaccgg tgcttatctt
    24061 ctcgctggag atgccatcag aacagatcat gatgcgttct ctggcgtcgc tgtcgcgcgt
    24121 tgaccagact aaaatccgta ccgggcagct cgatgatgaa gactgggcgc gcatttccgg
    24181 caccatgggt attttgctcg aaaaacgtaa tatctatatc gatgattcct ccggcttgac
    24241 gccaacggaa gtgcgttccc gcgcacgccg tattgcccgt gaacacggcg gcatcgggct
    24301 tatcatgatc gactacctgc aactgatgcg cgtaccggcg ctttccgata accgtacgct
    24361 ggaaattgca gaaatctccc gctcgctgaa agcactggcg aaagaactga acgtgccggt
    24421 ggtggcgctg tcccagttga accgttctct ggaacaacgt gccgacaaac gcccggtcaa
    24481 ctccgacctg cgtgaatctg gctctatcga gcaggatgcg gacttgatca tgtttatcta
    24541 tcgtgatgag gtgtatcacg aaaacagtga tttaaaaggc atcgcggaaa ttattatcgg
    24601 taaacaacgt aacggcccaa tcgggacggt acgcctgacc tttaacggtc aatggtcgcg
    24661 cttcgacaac tatgcggggc cgcagtacga cgacgaataa taattatttt atgaattagg
    24721 taattaaagc aaacacttat caaggaacac aaatgcaagc ggcaactgtt gtgattaacc
    24781 gccgcgctct gcgacacaac ctgcaacgtc ttcgtgaact ggcgcctgcc agtaaaatgg
    24841 ttgcggtggt gaaagcgaac gcttatggtc acggtcttct tgagaccgcg cgaacgctcc
    24901 ccgatgctga cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg cgtgcggggg
    24961 gaatcaccaa acctgtactt ttactcgaag gcttttttga tgccagagat ctaccgacga
    25021 tttctgcgca acattttcat accgccgtgc ataacgaaga acagctggct gcgctggaag
    25081 aggctagcct gaacgagccg gttaccgtct ggatgaaact cgataccggt atgcaccgtc
    25141 tgggcgtaag gccggaacag gctgaggcgt tttatcatcg tctgacccag tgcaaaaacg
    25201 ttcgtcagcc ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca aaatgtggcg
    25261 caaccgagaa gcaactcgct atctttaata ccttttgcga aggcaagcct ggtcaacgtt
    25321 ccattgccgc gtcgggtggc attttactct ggccacagtc gcattttgac tgggtgcgcc
    25381 cgggcatcat tctttacggc gtatctccgc tggaagatcg ctccaccggt gccgattttg
    25441 gctgtcagcc agtgatgtcg ctaacctcca gcctgattgc cgtgcgtgag cataaagccg
    25501 gagagcctgt cggttatggt ggaacctggg taagcgaacg tgatactcgt cttggcgtag
    25561 tcgcgatggg ctatggcgat ggttatccgc gcgccgcgcc gtccggtacg ccagtgctgg
    25621 tgaacggtcg cgaagtgccg attgtcgggc gagtcgcgat ggatatgatc tgcgtagact
    25681 taggtccaca ggcgcaggac aaagccgggg acccggtcat tttatggggc gaaggtttgc
    25741 ccgtagaacg tatcgctgaa atgacgaaag taagcgctta cgaacttatc acgcgcctga
    25801 cctcaagagt cgcgatgaaa tacgtggatt aatcgttctt taatatttga ttgtctgtgc
    25861 cggatgcggc gtgaatgcct tatccggcca ataaaatctt accaattcaa taagttgata
    25921 ttctttcatg ctcttataaa ggtcgtgcat ctggcggatg tacgtttgtc atgagtctca
    25981 ctctgttgct aattgccatt cgcccctgaa catcctctcg atcttcgcct tcttccggtt
    26041 tattgtgttt taaccacctg cccgtaaacc tggagaacca tcgcgtgttt caaaaagttg
    26101 acgcctacgc tggcgacccg attcttacgc ttatggagcg ttttaaagaa gatctccgca
    26161 gcgacaaagt gaatttaagt atcggtctgt actacaacga agacggaatt attccacaac
    26221 tgaaagccgt ggcggatgcg gaagcgcgcc tgaatgcgca gcctcatggt gcttcgcttt
    26281 atttaccgat ggaagggctt aacagctatc gccatgccat tgcgccgctg ctgtttggtg
    26341 cggaccatcc ggtactgcaa caacagcgcg tagcaaccat tcaaaccctt ggcggctcag
    26401 gggcattgaa agtgggcgca gatttcctga aacgctactt cccggaatca ggcgtctggg
    26461 tcagcgatcc tacctgggaa aaccacgtag caatattcgc cggggctgga ttcgaagtaa
    26521 gtacttaccc ctggtataac gaagcgacta acggcgtgcg ctttaatgac ctgttggcga
    26581 cgctgaaaac attacctgcc cgcagtattg tgttgctgca tccatgttgc cacaacccaa
    26641 cgggtgccga tctcactaat gaccagtggg attccgtgat tgaaattctc aaagcccgcg
    26701 agcttattcc atttcttgat attgcctatc aaggatttgg tgccggtatg gaagaggatg
    26761 cctacgccat tcgcgccatt gccagcgctg gattacccgc tctggtgagc aattcgttct
    26821 cgaaaatttt ctccctttac ggcgagcgcg tcggcggact ttctgttctg tgtgaagatg
    26881 ccgaagccgc aggccgcgta ctggggcaat tgaaagcaac cgttcgccgc aactactcca
    26941 gcccgccgaa ttttggtgcg caggtggtgg ctgcggtgct gaatgacgag gcattgaaag
    27001 ccagctggct ggcggaagta gaagagatgc gtactcgcat tctggcaatg cgtcaggaac
    27061 tggtgaaggt attgagcaca gagatgccag agcgcaattt cgattatctg cttaatcagc
    27121 gcggcatgtt cagttatacc ggtttaagaa ccgctcaggt tgaccgacta cgtgaagaat
    27181 ttggtgtcta tctcatcgcc agcggtcgca tgtgtgtcgc cgggcttaac gcgcaaaatg
    27241 tgcatcgcgt ggcaaaggcg tttgctgcgg tgatgtgatt ttaatcaaca ggcggatctc
    27301 tccgcctgtt cctgactcca gccacaccct actattctgt cccttttttc gcccactttt
    27361 acatcatctc ttttctataa gcttttgaaa attagtgttt ttaactgttg gtacggattg
    27421 tgcagtaatg cggctgtcta tgaccatcag gagaaaaaca tggagaacat gacctcgccc
    27481 ggcacgctgc ttagtggaga taacgcgacc tggcttgaag agtactacca gacctggttg
    27541 cgtacgccgg aaaaactccc ggaagactgg cggcgttttt ttctttcacc tgaactgact
    27601 gtccaaagcg tatccggtga taacaacgtc agcggtgcga cgttaaaaaa acaggctgcg
    27661 gtaatacaac tgatcaatgc ctggcgcacg caggggcatc tgcgcgcaaa gcttgatcca
    27721 cttggtttga atcccccggc tgacgttccc tccctccagc ctggtttttg gggattaagc
    27781 gaagaagact tgttgcagga atttagcgtc acctttggcg cacacaccac gcaaatgcca
    27841 cttaagcaac tgcttaacct gctggagcaa gcctgggcgg gtagctaggc ttatgaactt
    27901 gctcatctgg aaaatcgtga agaaatcaac tggttgttat cacggattga gagcagcaat
    27961 gcgccacaag cagatgtaca aacctgtatt gctcgttttg aaaaactgat ggcggcagag
    28021 acgttagagc gttatctgca tacccgttat gttgggcaga aacggttttc tctggaaggg
    28081 ggcgaaagtg caatcccggc gctcgatacg ctgacaaaac gcctgcgtgc gcaaggtgtg
    28141 gaggaaatgg tcattggcat ggcgcaccgg ggaagactta atgttctggt taatctgttg
    28201 aataaagacc cggcacagct ttttgctgag tttgaaggca agcagacgat cggcagtggt
    28261 tcaggcgatg tgaaatacca catggggtat tccagcaatc tggaaacacc tgccggttcg
    28321 ctgcatgtgg cgctggcgta taacccctca catctggaga tcgtgaatcc ggtggtgctg
    28381 ggccaggtgc gagccaggca ggaacgccgt ggtgaggatg gtcaggcaaa agtggtgggc
    28441 gtacttattc atggggattc cgcgttgggc ggcctcggcg tcaaccaaac cacgtttaac
    28501 ttatcgcaga ctcagggcta cggcactggc ggtacgctac atctggtgat taacaatcag
    28561 attggcttca ccacttcccg cttgcaggat atgcgttctt cacggtactg caccgatatt
    28621 gccaaaatgg tagcggcccc aattatccac gttaatggcg atgacgttga cgctgtttgc
    28681 caggtaatgg aactggcgtg cgaatggcga gacaccttcc ggcgcgacat catcatcgat
    28741 atttgctgct tccgtaagca cggacacaac gaaagcgatg agccacgtct tacccagcca
    28801 caaatgtatc aggcagtgga tgctcaccct ggtacgctcg cccgttatgg tgaatctctt
    28861 gcccgccgcg gcctgttaac tcaggcgcaa caagatgaga tgacggcacg ctatcgcgac
    28921 tggctggata gctgccagaa acgtgaaccg cagcctctca aacctgcaat tcactctttt
    28981 agcgctaact ggtatgggtt gaccaaccct cactggagcg ccccggtatc tacggcattg
    29041 cctcggcaaa agctggcagc gtatggtgaa atcatttcta cactgccacc cgatgtggtc
    29101 gcacatccta ccattaaacg gcaactggcg ttacgccagg atatggccgc aggtactcag
    29161 ccaatagact ggggaatggc tgaaatgctg gcctatgcat cgcttgtgga tgcgggtgtt
    29221 ggtgtgcgtc tttctggtga agactcaggg cgtggcacgt tcagtcatcg ccatgcagtg
    29281 gtacatcatc aaactgaagc ccgtcgttat ctgccgttac agcatatccg cgctggtcag
    29341 gcatctttcg atgtttatga ttcggtgctt aatgaagaag cgctcttagc ctttgaatat
    29401 ggctattcaa cgtctgcgcc tcagcaactg gttatttggg aagcgcagtt tggtgacttt
    29461 gccaacggcg ctcaggttgc tattgaccag tttatctcgt ctggcgaaac caagtgggat
    29521 cgctatagcg gtttaaccat tcttctgccg catggctatg acggacaagg gccggagcac
    29581 tcttctgcgc gtcctgagcg ctggctgcaa ctgtgcgcag aaaacaacat gcaggtggtg
    29641 atgcccagtg agtcagcaca aatgttccat ttgctgcgcg gtcaggcatt acgcccaatg
    29701 cgcaaaccgt tagtgataat gatgagtaag cgtttacttc gttttaaagg cgctatgagc
    29761 gagctttgtg aattcactga tggcgcttat aaaccggtaa tcaccgatcc gcaattgcat
    29821 cagtcccaga aagttaagcg cgttattttg tgtagcggcc aggtctatta cgacgtactg
    29881 gaagcccgta agcagcgtga gcatgaagac gaagtggcga ttgttcgcct ggagcaactc
    29941 tacccgttcc cggtcgcaga actgaatgat gttctggcga gttggccgaa ctgctgcgaa
    30001 tggatttggt tgcaggaaga gccagaaaac cagggcgcgt ggcggcagat tcgtcatgag
    30061 ctggcggcac tgaaaattaa cactccttac tggcagtatg caggccgccc ggcagcggca
    30121 gctccggcga cagggtatgg gcgtgtacat aaacaacaaa tcgacgaatt tcttgctgct
    30181 gcgtttgcag atattcagcc atgaaaggcg cacaaaaata ataaggagaa cattttatga
    30241 tcgaaattac tgtccctgta ttaccggaat ctgtcacgga aggcacgctg acaacctggt
    30301 gtaaacaaga aggcgagcac gtaaaacgcg atgatgtgat tgccgagctg gaaaccgata
    30361 aagtcattct ggaaataccg gccccgcacg atggcgtgtt aagcaatatt atcgtcagcg
    30421 aaggtagcac ggtcacgtcc gcgcaactgt tggcgcatct taagccgcaa gctgtcatag
    30481 aagaaaccgt cacaccagtc accgaaaccc tggcgatgcc ttccgcacgg ctggaggcac
    30541 agcgtagtgg tgtcgaactt gctgacgttg cggggagcgg gagaaatgga cgtatcctga
    30601 aagaggatgt acagcgcgtt actcctgcac cagtcattca gcctgaacgg gtggcggaga
    30661 tcgcgccagc taaacccttg acaccgggtg ctcgtcagga acgtcgtgag ccgatgtcgc
    30721 gcttacgtca gcggattgct gagcgtctgc tggcttccca acaaaataac gccattctta
    30781 ccacattcaa cgaagtaaac atgcagagcg tgatggattt acgcgcccgc tggaaagatc
    30841 gttttgccga gaagcacggc gtgaagctgg gctttatgtc cttctttgtt aaggccgtta
    30901 cccgggcgct ggagcgcttc cctgtagtga atgccagcgt tgatggcaac gagattatct
    30961 ggcgtgatta ttgcgatatc ggcattgcag tgagcagcaa ccgcggcctg gtagtgcctg
    31021 tgttacgcaa tgcgcaatca ctctctttgg tggaaattga acggcaaatt gccgaatacg
    31081 ccacacaggc acgcaatggc aaattgccgc tggaggcgtt acagggcggc acgttctcta
    31141 tcaccaacgg cggtactttt ggttcgatga tgtcaacgcc aatcattaac ccgccgcagt
    31201 cggccattct tgggatgcat gccatcacgc cgcgcccggt cgcagaaaac ggccaggttg
    31261 ttattcgtcc catgatgtac cttgctctca gctatgacca tcgcattatc gatggtcagg
    31321 aagccgtgca gacattggtg gcaatacgtg agctactgga atcaccggaa caactgttgc
    31381 tggatcttta aggagggcgc cgatgagtac aatttttgat gtggcagtta tggggggggg
    31441 ggccaggtgg ttacgtagcg gcactgcggg ctgcgcagaa tgggctttcc gtagtatgta
    31501 ttgatgatgg tgttaatgca cagggcgaac cttcgccggg cggtacgtgt ctcaatgtcg
    31561 gctgtattcc gtcaaaatcg ttgctgcaat cttccgaact gtacgcgcaa gtgcagcatg
    31621 aggcgagcat tcatggcgtt aatgttgacg gcgtatcttt caatgcagct gcgatgattc
    31681 aacgtaaaga cgccatagta agccgcctga caatgggcat cggcttgctg tttaagaaaa
    31741 ataaagtgaa gtacctgtgc ggcctcgcaa cgttagaaca tgctcgggat gagatttggc
    31801 aattacgtgt aaacgatcaa caaatccgcg cccgtaatgt ggtgattgca acaggttctc
    31861 agccgcgtca actgcctggc gttaccattg ataatcagca aattctcgat aatcgcggcg
    31921 ccctggcgtt aaccgctgtg ccgccgcgtc tcggtgtgat cggcgctggc gtgattggcc
    31981 ttgaactggg gtctgtgtgg aatcgggtcg gttcagacgt cacgttgctg gagatggcgc
    32041 caacgttctt gcctgcactg gaagctcgcc tgtcgaacga agtccgtaaa gcaatgattg
    32101 ccagtgggat gaaaatgcaa ctggcagtag agattgaggc tattgaacaa cgggatgacg
    32161 gtgtgcatgt gcgctggcgg cagggtgaga agcgagaaga aagccgcttc gataaattaa
    32221 ttcttgccat tggacgtgta ccgcgacttt ctggtgtcga tctcgtgcaa ctcggtcttg
    32281 aagccgacaa ccgtggcggt attgccgtcg ataacttatg ccgtaccggg aaagccgggc
    32341 tgtgggcaat tggtgatgtc gtgcgagggc cgatgctggc gcataaagcg atggctgaag
    32401 gtgtggtggt tgcggatcaa attgccggac tggcggtaga gccgattaat tttgcgctga
    32461 tcccttcggt gatttacacc caaccggaag ttgcgtgggt aggggaaaac gaggcctcac
    32521 tgaaagccgc tggcagagtc tttaacaaag gaaattctct gtttgccggt aacggtcgcg
    32581 ccttagcatt aggccaggaa ggtgggcgtt gcacgttata cagcgataaa cacactgacc
    32641 gcgtacttgg cggagcgatt gttggcccgc aggcatcgga gctgatcaat gaaattgcgc
    32701 tggcgatgac ttttagcgcc tctggcgagg atattgcctg tgcgatccat gcgcatccga
    32761 ccttaagcga agtcatccac gaagcagcaa tggccttgaa taataaagca ttacacggat
    32821 aacacatacc actgagggaa tatatgaatt tacatgagta tcaggccaaa tcgttactgg
    32881 ctggcatggg gatgccgtgt ccaaaagaga tagccatcca acagatttcg caactggcag
    32941 atgcctggca acatattgct tgcccgagta aaggcgcggt acttaaagcg caggtacatg
    33001 ctggtgggcg cggtaaagct ggcggcgtaa aagtgcttaa gcagctgccg gaagcacagg
    33061 cttttgtaca gcaaatgttg ggatcgcaac tggtgactta tcagaccggg ccagaagggc
    33121 agtatgtcag cagtattttg ctgtgcgaaa acatctatcc ggtacgccag gaactctatt
    33181 ttggcatggt ggtggatcgt gaaagccagc gggtcacgtt tattgtcagc ccggaaggtg
    33241 gtgtggaaat tgaaaaagtc gcccatgaga caccggagaa aatcagcagt gtcagcattg
    33301 atccactgac aggcgtgcag ccttgccata tccgcgaaat gtttgctgtt ctgcaactgg
    33361 aacacgggct atttgctgcc tttagtcgcc tggttaatca ggcgtggaaa gcctttaacg
    33421 aactggattt cgccctgctg gaaattaacc ctctggtgtt gcgggaaacg ggcgaattta
    33481 tgtgtgcaga cgcgaaagta tcgctggatg ataacgcgct gtatcgtcac ccggaactcc
    33541 aggtgctacg tgatgaaacc caggaagatc cacgcgaaag ccaggcagcg aagcttgatc
    33601 ttaattatgt ctcgctggac ggaaatatcg gctgcatggt caatggtgca gggctggcta
    33661 tggcaaccat ggacatcatc aagctatacg gtgaacaacc cgcaaacttc ctggatgttg
    33721 gcggtggcgc aacccaggag cgcgtcagtg aagcctttcg gctgattgtt tccgacagca
    33781 aagtgaaagc cattctggtt aatatttttg gtgggattgt ccgttgcgac atgatcgcca
    33841 gagccattat tcatgctctg aatgaggccc gtatcaccct gcctgtcgtg gtgcgtctct
    33901 ccggtaataa cgctgccgaa gggcagcggt tactggcaga aagtggcctg acggtggaag
    33961 ccgtcaattc tctggatgat gccgctaaac gcatcattgc gttactgaat taacaggagg
    34021 ttaagatgag cgttttgatc aacaaacaca cgcgggtact ggtgcagggc attaccggca
    34081 aaaacggcac atttcataca gagcaggcta ttgcgtatgg cacgcagatt gtgggtggag
    34141 tgacgccagg taagggcggg caacggcatc tggatcgccc ggtttttgat tcaatgaaag
    34201 aagcgatgcg acaaactgag gctgatgcca gcgtgattta tgtgcctgca ccgtttgtgc
    34261 tggattctgt tgttgaggct attgaagctg gcgtcaaatt ggttgtggtg atcactgaag
    34321 gtgtaccgac actggatatg gtgaaagttc gtcggctgct tgactgccac ccggatgtgc
    34381 ggctgattgg gccaaactgc ccaggagtta tcacgccggg cgagtgcaaa atcgggatta
    34441 tgcctggtga aatccaccgc ccagggcgta ttgggattgt ttcccgctcc ggtacattaa
    34501 cctacgaagc tgtagcgcaa accacggcgc tgggacttgg gcaatccacc tgtattggta
    34561 ttggcggcga tccggttccg gggactaact ttattgatgc gctgcgttta ttcgaaaatg
    34621 atccgcaaac agacgcggta attatgatcg gtgaaattgg tggtaatgcg gaagaacggg
    34681 ccgcggaatt tattcgtgat gaaatgaata aaccggtcgt aggttatatt gccggtgtta
    34741 ctgcaccaaa aggaaaacgc atggggcacg ccggagccat tatttctggc ggtagcggca
    34801 gtgcagagga taaattccgt gcgttcgata aagcaggcat ggcatggaca agaaacccgg
    34861 cgttgcttgg cgaaacgtta tggaatgtta ttaagtaatc cataaatgaa tatcaaggga
    34921 gcatatcgct cccttttttt atctgtgtgg taagagaatc accttttatt tactgtgcgg
    34981 aaaaccgcgc acaaaataaa aagaataaat aataaaatca ttaaataac
//
LOCUS       DADTFG010000034        34923 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.034, whole genome shotgun sequence.
ACCESSION   DADTFG010000034 DADTFG010000000
VERSION     DADTFG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.034"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            40..780
                     /locus_tag="J1138_001548"
     CDS             40..780
                     /locus_tag="J1138_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBA8453363.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            815..3403
                     /locus_tag="J1138_001549"
                     /pseudo
     CDS             815..3403
                     /locus_tag="J1138_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
     gene            3420..3986
                     /locus_tag="J1138_001550"
     CDS             3420..3986
                     /locus_tag="J1138_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8453364.1"
                     /translation="MIKITPHKITILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     GTNVTDNGNDKYTLVIPSMGMDKIANKKAQSEANFKLVANGCSSGISWIDTTLTGNQS
                     GSSPALIIPLASDTTSTTSYIGMGFKRKATSGDTFLKPNSAEYIRWSASEISTDGLEM
                     TVALRETSVGKGVPGKFRALATFNFSYQ"
     gene            3998..4606
                     /gene="yadL"
                     /locus_tag="J1138_001551"
     CDS             3998..4606
                     /gene="yadL"
                     /locus_tag="J1138_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBA8453365.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDVEMG
                     TCTSTLTDDGTTKISTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTTR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAN
                     ATGSNVVDYPMSAVLVVAKDKTVTNVTAGDFTAPATFTVTYN"
     gene            4633..5229
                     /locus_tag="J1138_001552"
     CDS             4633..5229
                     /locus_tag="J1138_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8453366.1"
                     /translation="MRPTQRNLMKKSILILSLLFSATSPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHINF
                     RALSGVMATGSSQIFANEDNAGAKNVGVVIFSIQDPANTFNVLSATGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGPLISHVLVDIYYE"
     gene            5279..6541
                     /locus_tag="J1138_001553"
     CDS             5279..6541
                     /locus_tag="J1138_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBA8453367.1"
                     /translation="MKTFFRYFLFLALCSCCYTASAGTDDDVGYIVGNNYGVGPSDQK
                     WRETGPNGDVTVKFRYGSGTNNLVFYKPTQLGPTGVSLKWAQLDSASGGGFLYCNRSS
                     NSSGAPMSIEHKMVDSGKSYGGHKLFKTSVPGLYYTLAISNIWSTLTSTDINPSGMYI
                     GDSTSQSFNWRGESEQTLYWSCNNANSSKKYWAVGGVMQTLTIEFYTDTDFNPTTNQR
                     VTLSRTDSYLYSFKAYNAGVSIKSYFLKIDFDLTDIVLTNPTCFTAALSGPSVSGSTV
                     KMGDYSPAQIKNGATAVPFDITLQNCIRVRNIETKLKSNKVGSVSKELLANTLTGNDA
                     AKGVGVLIEGLKNTKSAQMVLKPNDATSIYKDYETENDTTGGIFPDNGNAGTSQPLHF
                     QATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            6653..7447
                     /gene="panB"
                     /locus_tag="J1138_001554"
     CDS             6653..7447
                     /gene="panB"
                     /locus_tag="J1138_001554"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBA8453368.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7459..8310
                     /gene="panC"
                     /locus_tag="J1138_001555"
     CDS             7459..8310
                     /gene="panC"
                     /locus_tag="J1138_001555"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBA8453369.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKLVELA"
     gene            complement(8664..9596)
                     /locus_tag="J1138_001556"
     CDS             complement(8664..9596)
                     /locus_tag="J1138_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBA8453370.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGSTGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVIQEVRQEV
                     RQEYALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            9870..10250
                     /gene="panD"
                     /locus_tag="J1138_001557"
     CDS             9870..10250
                     /gene="panD"
                     /locus_tag="J1138_001557"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBA8453371.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10254..11483)
                     /locus_tag="J1138_001558"
     CDS             complement(10254..11483)
                     /locus_tag="J1138_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBA8453372.1"
                     /translation="MYKQAVILLLMLFTASVSAALPAHYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11547..11987)
                     /locus_tag="J1138_001559"
     CDS             complement(11547..11987)
                     /locus_tag="J1138_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA8453373.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLTSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12092..12862)
                     /locus_tag="J1138_001560"
     CDS             complement(12092..12862)
                     /locus_tag="J1138_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8453374.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12859..13785)
                     /locus_tag="J1138_001561"
     CDS             complement(12859..13785)
                     /locus_tag="J1138_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8453375.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            13894..14556
                     /gene="can"
                     /locus_tag="J1138_001562"
     CDS             13894..14556
                     /gene="can"
                     /locus_tag="J1138_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBA8453376.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHIN
                     HK"
     gene            complement(14597..15133)
                     /gene="hpt"
                     /locus_tag="J1138_001563"
     CDS             complement(14597..15133)
                     /gene="hpt"
                     /locus_tag="J1138_001563"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBA8453377.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDD"
     gene            15339..17729
                     /locus_tag="J1138_001564"
     CDS             15339..17729
                     /locus_tag="J1138_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/quinate/shikimate family membrane-bound
                     PQQ-dependent dehydrogenase"
                     /protein_id="HBA8453378.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17776..19326)
                     /gene="cueO"
                     /locus_tag="J1138_001565"
     CDS             complement(17776..19326)
                     /gene="cueO"
                     /locus_tag="J1138_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBA8453379.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPSGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAITPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19492..19839
                     /locus_tag="J1138_001566"
     CDS             19492..19839
                     /locus_tag="J1138_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBA8453380.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19945..20811
                     /gene="speE"
                     /locus_tag="J1138_001567"
     CDS             19945..20811
                     /gene="speE"
                     /locus_tag="J1138_001567"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBA8453381.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20827..21621
                     /gene="speD"
                     /locus_tag="J1138_001568"
     CDS             20827..21621
                     /gene="speD"
                     /locus_tag="J1138_001568"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBA8453382.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21659..22021)
                     /locus_tag="J1138_001569"
     CDS             complement(21659..22021)
                     /locus_tag="J1138_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBA8453383.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVED
                     AARTLKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22196..24793)
                     /gene="acnB"
                     /locus_tag="J1138_001570"
     CDS             complement(22196..24793)
                     /gene="acnB"
                     /locus_tag="J1138_001570"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBA8453384.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            25148..26905
                     /locus_tag="J1138_001571"
     CDS             25148..26905
                     /locus_tag="J1138_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBA8453385.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIIATTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRHAAASQFQQRTHAAPVTTRDTQRQAAAQRFNEAEHYGNYDDFRDFS
                     HHQPLTQQQKDAARQRYQSASPEQRQAVREKIQANPQNQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(27147..28571)
                     /gene="lpdA"
                     /locus_tag="J1138_001572"
     CDS             complement(27147..28571)
                     /gene="lpdA"
                     /locus_tag="J1138_001572"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBA8453386.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28779..30671)
                     /gene="aceF"
                     /locus_tag="J1138_001573"
     CDS             complement(28779..30671)
                     /gene="aceF"
                     /locus_tag="J1138_001573"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBA8453387.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMIFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30686..33349)
                     /gene="aceE"
                     /locus_tag="J1138_001574"
     CDS             complement(30686..33349)
                     /gene="aceE"
                     /locus_tag="J1138_001574"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBA8453388.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33510..34274)
                     /gene="pdhR"
                     /locus_tag="J1138_001575"
     CDS             complement(33510..34274)
                     /gene="pdhR"
                     /locus_tag="J1138_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBA8453389.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(34730..>34923)
                     /locus_tag="J1138_001576"
     CDS             complement(34730..>34923)
                     /locus_tag="J1138_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA8453390.1"
                     /translation="KGKKLEKYLDTLVDHFIKITEHPKKGDLIFYPNSPEDGKPENIL
                     KIVKEWRRSQGLPLFKDSE"
BASE COUNT         8826 a         9092 c         8339 g         8666 t
ORIGIN      
        1 attattccct gttttaatta attctgtaag gaatggttta tgttttttaa tactaaacat
       61 accgcagcat tgtgcttagc gagctgtatg gcttttagct catcagctat tgcagacatc
      121 gtaatttcag gtacacgcgt agtttataaa agcgaacaaa aaagcgtcaa tgtacgtctg
      181 gaaaataaag gcaataaccc attgctggtt cagagttggt tagatactgg agatgataac
      241 gctgaaccag gcagtattac tgtccccttt accgctacgc cgccagtatc acgtattgat
      301 gctaaacgtg gccaaacaat caaactgatg tacaccgcca gcagtgctct gccaaaagac
      361 agagaaagtg tgttctggtt taacgtactg gaagtacctc caaaaccaga tgcagcaaaa
      421 gcagcaaacc aaagcctgct acaactggca tttcgcacac gtataaaact gttctatcgc
      481 ccggaaggat tgagtggaat ttcatctgat gcaccattgg ctctgaagtg gtcctgggca
      541 acatctgaag gcaaagcagc attacgcgtt gataacccta cgccctacta tgtctctttt
      601 agcagtggtg atttagaagc caacggcaaa cgctatcccc tggatatgac aatgattgcg
      661 ccgttcagta acgacgtcat caaggtaaat gggatgagtg gcagaacaag ctctgcaaaa
      721 gtgcattttt tcgccattaa tgattttggt ggatctattg aaggcaacgc caacctttaa
      781 tctggcaagg atataacaca gcaggaagca gaacgtgact attaaatcta ctaatcacct
      841 tacccacatc gccacgtttt gcgctctgct gtatagcaat agtgctttat gcgctgaact
      901 cgtcgaatat gaccatacat ttctgatggg aaaggatgct tcaaatatcg accttagtcg
      961 gtatacagaa ggcaatccca cactacctgg catttacgat gtcagtgtgt atgttaacga
     1021 tcaacccatc atgagccaaa gcattgcttt tgctgttatt gaaggtaaaa agaacgctca
     1081 ggcctgcatt acgcagaaaa atttattgca atttcatatc agttcaccag acaagaatag
     1141 cgaaaaagcc attttgctta agcgagacga agatcttggt gattgcctca atctggctga
     1201 aatgatccct caatcatcca ttcgttacga tgttaacgat caacgcctgg atatcgatgt
     1261 cccccaggca tggattatga agaattatca aaactatgtt gatccctctt tgtgggaaaa
     1321 tggcattaat gcagccatgc tttcctacaa cctcaacgga tatcacagtg aaagtccagg
     1381 cagaacaaat gacagtatct atgcggcatt caacggtggg ataaacttag gtgcctggcg
     1441 gttacgtgcc tcgggtaact acaactggat gaccaatgtc catagcgatt acgattttca
     1501 gaatcgctat ttacaacgcg atcttgcgtc actacgttca caactggtta ttggtgaatc
     1561 ttacacaacc ggcgaaacct tcgattcagt cagtattcgc ggtattcgct tatacagtga
     1621 cagtcgaatg ctgcctccgg tattagccag ttttgcgcca atcattcatg gcgtagcgaa
     1681 taccaatgcc aaagtaaccg ttatgcaaaa tggttataaa atctatgaaa cgacggtgcc
     1741 gccaggggcc tttgccattg acgatcttag cccgtcaggt tatggcagtg acctcattgt
     1801 gactatcgaa gaagctgatg gcacaaaacg gacgttctcc cagccatttt catctgttgt
     1861 gcaaatgctg cgccctggcg ttggtcgctg ggatattagc gccggtcagg ttttaaaaga
     1921 cagtatccag gatgagccta atttatttca agccagctac tactatggtt tgaataacta
     1981 cctgacagga tataccggta ttcagctaac tgataataac tacaccgccg gattgctggg
     2041 gcttggtatg aatacccccg tgggggcttt ttctgtcgat gttactcact caaatgtcag
     2101 cattccggac gataaaacct atcaaggaca aagctaccgt atttcctaga acaagttatt
     2161 tgaaaatacc agtacatcat taaatatcgc ggcctatcgc tactccaccc agcactatct
     2221 gggcctcaat gatgcgctga cgctgattga tgaagtagaa catcctgagc aagagcttga
     2281 acctaaatcc atgcgcaatt actcgcgcat gaaaaatcag gtcacggtca gtatcaacca
     2341 accgctgaaa tttgagaaaa aggattacgg ttcattttat ctctcgggaa gctggtcaga
     2401 ttactgggcc tccgggcaaa acagtactaa ttactctatt ggttatagca atagtgcgtc
     2461 ctggggcagt tacagtatca gtgctcaacg ttcgttgaat gaagatggtc aaaccgacga
     2521 tagcatttat ctcagcttca cgattccaat agaaaattta ctgggtacgg aacaccgcag
     2581 ctccggcttc cagagtattg atacacaatt aaacagcgac tttaagggta ataaccaact
     2641 caatatcagc agtagtggtt acagcgatac caatcgcatc agctacagcg taaacacggg
     2701 ttatatgatg aataaatcca gtgatgattt gagttatatc ggcggttatg ccagttatga
     2761 atcgccgtgg ggaacgctgt caggttcagc ctcagcaagc agtgataata gtcgccaatt
     2821 ctcactcaat actgatggtg gatttgtgtt acatagcagc gggctgacgt tcagtaatga
     2881 cagttttagt gattccgaca ctctggctgt cattcaggcg ccaggagcaa aaggcgctcg
     2941 tattaactac ggaaacagca ccgtagaccg ctggggttat ggtgtgacca gcgccctttc
     3001 gccataccat gaaaatcgta tcgcactgga cattaacgat ctggaaaacg atgttgaatt
     3061 gaaaagcacc agtactgtcg ccgtcccgcg ccagggagcc gtcgtctttg ctgattttga
     3121 aacagttcag ggacaatcgg caattatgaa catcgtgcga agcgatggta aaaatattcc
     3181 atttgcagcg gatatttatg atgaacaaaa caatatcatc ggcaacgtag gtcagggtgg
     3241 tcaggccttt gttcgcggta tcgggcagga aggaaatata cgaattacct ggattgaaga
     3301 gggcaaaccg gtcagttgtt ttgctcatta tcaacaaaac acgacatcag aaaaaatagc
     3361 acaatctatt attctgaacg gacttcgttg tcagatacag taaccacaag ggacttccaa
     3421 tgataaaaat cacaccacat aaaataacaa tactcatggg actattgtta tcgccttcgg
     3481 tatttgcaac ggatgttaat gtcgatttta ccgctaccgt gaaagcaaca acctgtaata
     3541 ttacacttac cggaactaat gtcactgata atggaaatga taagtacacc ttagttattc
     3601 cctcgatggg aatggataaa atagccaata aaaaagcaca atctgaagcc aactttaaat
     3661 tggtagccaa tggatgcagc agtggaatta gctggattga tacaaccctg acaggaaatc
     3721 aatcaggaag ttcacccgcg ctaattatcc ccctggccag tgatacaacc tccaccacaa
     3781 gctatatcgg aatgggattt aaaaggaagg cgacttcagg cgatacattt ctgaaaccta
     3841 atagcgcaga atatatacgt tggagtgcat cagaaattag caccgatgga cttgagatga
     3901 ctgtcgcact gcgtgaaaca agtgtaggga aaggtgttcc aggcaagttt cgcgcactgg
     3961 caacttttaa cttcagctat caataacaag gatgacaatg atgatattaa aaaaattatg
     4021 ttatggatta tcgggtagtc tatttctggc ttcatcactg ttaaccattt cctcacatgc
     4081 ggccactgac agtattggcc tgactgttac tactgacgta gaaatgggta cctgtacatc
     4141 aacgttgact gatgacggta caactaaaat ttccaccatc aatttcggtg atgtatatat
     4201 ttctgaaatt aatgccgaaa ctaaaattaa aaccttcaaa ttacagttca aagactgtgc
     4261 ggggattccc gggaaaaagg ccaaaataaa attgactaca cgagcattat gtgaaggtaa
     4321 ttctaataat ggtcctggat ttgctaacgc atcaacggca acagcaaaag cagcggcggt
     4381 cgccgttgag gtctggagca catccacacc aggtaagaac ggcgctaaac aattcagttg
     4441 tgtcaccccc gcaacagaag aagtcagtat tgctaatgcg acgggcagca atgttgtcga
     4501 ttacccaatg agtgcggtgt tggtggtcgc aaaagataaa actgtcacaa atgtcactgc
     4561 gggtgatttc actgccccgg caacattcac cgtgacttat aactaacagg cagactgatt
     4621 caggaactaa atatgcgtcc cactcaacgt aatctgatga aaaaatcaat tctgattctg
     4681 tcgttgctat tcagcgccac ttcccctgtg gttgcaggac aggatgttga cattgttgca
     4741 aatataaaaa ataacacctg ccaaagtgga atcagcaaca acggaaatat tgatttaggt
     4801 gtcgttggcg tgggcgattt taccggaaac atttcagccg aaaattatca ccccggtgga
     4861 aaagagttca ctattacagt acaggactgc acacttcagg gcacagggaa tgtgttaaat
     4921 cagttacata ttaattttcg cgcacttagt ggcgtaatgg cgacaggttc cagtcaaata
     4981 ttcgctaatg aagacaacgc cggggctaaa aatgttgggg tagttatatt ttctattcag
     5041 gacccggcaa ataccttcaa cgttcttagt gccactggta gctcacgttc cgtttatccg
     5101 gtcatgtcat cagcactaaa taattcatcc tggaaattta gtacccgaat gcaaaaaatc
     5161 gatccagcac tgaatgtcac ctctggccca ctgataagcc atgtgttagt ggatatctac
     5221 tacgaataac agacaaagtg ctacaagtat tatcttgcag catgacaatg gagtaaaaat
     5281 gaagacattc ttcagatact ttcttttttt agcactatgc tcttgttgtt atacagcaag
     5341 cgccggcacc gatgatgacg tcggctatat tgttgggaat aattacggtg ttggtccatc
     5401 agaccaaaaa tggcgagaga caggtcccaa tggagatgtt accgtcaaat tccggtatgg
     5461 ttcaggcacc aataatttag ttttttataa accaacccaa cttggcccaa ccggggtgtc
     5521 attaaaatgg gcgcagttag attccgcttc gggtggaggt ttcctttact gtaacaggag
     5581 tagtaactca agcggcgcac caatgtctat tgaacataaa atggttgatt ccggaaaaag
     5641 ttatggcgga cataaactct ttaagacatc tgttccaggc ctgtattaca cgttagcaat
     5701 atcaaacatt tggtcaacac ttacatctac tgatatcaac ccatcaggca tgtatattgg
     5761 cgattctacg agccaatctt ttaactggcg tggcgaaagc gagcaaaccc tatattggtc
     5821 ttgcaacaac gcgaatagca gcaaaaaata ctgggccgtt ggtggcgtta tgcaaacgct
     5881 cacgattgaa ttttataccg ataccgactt taacccgaca acgaatcaac gggtaacatt
     5941 atccagaaca gatagttatc tctactcatt caaagcatat aatgctggcg ttagtatcaa
     6001 gagttatttt cttaaaatcg actttgactt gacagatatc gtattaacaa accctacctg
     6061 ttttactgct gcgcttagtg gcccatcggt aagtggttca acggttaaaa tgggtgatta
     6121 tagccctgca caaataaaaa atggcgcaac agcggttcct tttgatatca cgctacagaa
     6181 ctgtattcgt gttcgcaata tagaaacaaa actcaagtca aacaaggtgg gtagtgtaag
     6241 caaagaactt ctggctaaca cactcacagg aaatgacgca gcaaaaggcg ttggtgtact
     6301 gattgaaggt ttaaaaaaca ctaaaagtgc acaaatggta ctgaaaccaa atgatgcaac
     6361 gtctatttat aaagattatg aaacagaaaa tgacactact ggggggattt tcccggataa
     6421 tggcaacgct gggacctctc aacctcttca tttccaggct acattaaaac aagacggtaa
     6481 tattgctata gaaccggggg attttaaagc caccagcact tttcaggtta cctacccctg
     6541 ataacaccat tgcaccgcca gacaacggcg gtgcgatacg gataaatcat tgcccccacc
     6601 aatgtgactg ataaaatgcg agacagcaca acatcattta tcaggatacg ctatgaaacc
     6661 aaccacgatt gcttcattgc agaaatgcaa gcaggacaaa aaacgtttcg caaccatcac
     6721 cgcttacgac tatagcttcg caaaactgtt tgccgaagaa ggacttaacg tcatgctggt
     6781 cggcgattca ctgggcatga cggttcaggg gcatgactcc acgctaccgg ttaccgttgc
     6841 cgatatcgcc tatcacaccg ccgctgtgcg tcgcggcgcg ccaaactgcc ttctgctggc
     6901 tgacctgcca tttatggcgt atgccacgcc ggaacaagcc tttgaaaacg ccgcaacggt
     6961 aatgcgtgcc ggagccaaca tggtcaaaat tgaaggcggt gagtggctgg tggaaaccgt
     7021 acaaatgctg accgaacgtg ccgttccagt ttgtggtcat ttaggcctaa ccccacagtc
     7081 agtgaatatt ttcggtggct acaaagtcca ggggcgcggc gatgaagcgg gcgatcgact
     7141 gctcagcgat gcattagcct tagaagctgc tggggcacaa ctgctggtac tggagtgcgt
     7201 gccggttgag ctggcaaaac gcattaccga agcactggca atcccggtta tcggtattgg
     7261 cgcaggcaac gtcactgacg ggcaaattct cgtgatgcac gacgcgttcg gcattaccgg
     7321 cggtcatatt cctaaattcg ccaaaaattt cctcgccgaa acgggcgaca tccgcgcggc
     7381 tgtgcggcag tatatggctg aagtggagtc cggcgtttac ccgggcgaag aacacagttt
     7441 ccattaagga gtgacgttgt gttaattatc gaaaccctgc cgctgctgcg tcagcaaatt
     7501 cgccgcctgc gtatggaagg taagcgcgtg gcgctggtgc ccaccatggg taacctgcac
     7561 gatggccata tgaagctggt cgacgaagcc aaagcccgcg ccgatgtggt cgtcgtcagt
     7621 attttcgtta acccaatgca gtttgaccgc ccggaagatc tggcacgtta tccacggacc
     7681 ttgcaagagg actgcgaaaa gctgaacaaa cgtaaagtgg atttagtttt cgcgccttcg
     7741 gtaaaagaga tctacccgaa cggtactgaa acccacacct atgttgatgt tcccggtctt
     7801 tccaccatgc tggaaggtgc cagccgtccg ggacattttc gcggcgtttc gactattgtc
     7861 agcaagctgt tcaacctggt ccagccagat atcgcttgct tcggtgaaaa agattttcag
     7921 caactggcgc tgatccgcaa aatggttgct gatatgggct ttgatattga gattgtcggc
     7981 gtaccgatta tgcgcgcgaa agacggtctg gcgctcagtt cccgtaacgg ctatctgacg
     8041 gcggaacaac gcaaaattgc gcccggtctg tacaaggttt taagttcgat tgccgataaa
     8101 ttacaggctg gtgaacggga tctcgatgaa attattgcta ttgccgggca agaactgaat
     8161 gaaaaaggct tccgcgccga tgatattcag attcgcgatg ccgacacatt gctggaggtc
     8221 tctgaaaaca gcaaacgcgc agtgattctg gtagccgcct ggcttggcga tgctcgcctg
     8281 atcgacaaca aactagtcga actggcgtaa tgcttaactg gcgctacggc tgatggcgcc
     8341 agttattcat tgccacgtta tctgtaggca attattcata tcgtagacaa cttagttaga
     8401 gttaattagc ttatcaatct ctgcaagaga taaagctgtc acctctgcaa cgtcttcccg
     8461 agacattcct ttactcagaa gacgctgggc cagtcttacc ttctggagtc cctctttata
     8521 tcccttctga aatcccttct cttcaaacca ctgcgccaac gtctgcctgg tttctctccg
     8581 tgtctttatt tccatatgtt cccttaatta tctggtcagt taacaataac aattatctgg
     8641 ctataactga taatgcagat aacttagcta atcagcttat caacctcagc aagaggtaaa
     8701 tttgccatct ctgcaacgtc ttcccgagac atccctttac tcagaaaacg cagggcgtat
     8761 tcctgtctta cttcctgtct aacctcttgt attacctcct gtcttacctc ctgtcttacc
     8821 tcctgccttt cctcctgtct tcccttctct tcaaaccact gcgccagcgt catcatagac
     8881 tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga
     8941 ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt gtacccttcg
     9001 tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt
     9061 tgcagcagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgata
     9121 gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagttc cggtgagtaa
     9181 aacatatcaa accagcacat tgatagcgga taaggtgtgg cctcgccctg ataaaacaga
     9241 attggcacca ccagcggcag cttatcgtga tcagcctcca gatgccggtg catggcagca
     9301 atagaataac gcatcatgcg aaaggccatt ttcttatctg gcttgctttg gtgttcaatc
     9361 acaacatgca gataacccgt actaccctgc atctgtacgg aatagagcac gtcagtgctg
     9421 tgccctttca ggctttcttc aataaaactc cccgactcaa gatgcagcgt gtcgaggtca
     9481 cacagttcgc gtagctccgc tggcaaatgg atatccagaa agtcgcgagc cgtttccgca
     9541 tgcattaaaa attgtttaaa caccgcatca tgcggtgtgg tactcggtgc atccatcttc
     9601 cctgccttcc gtgtggtctt gcgtgaaggc agtatccagt catggtgtgc cgtcgtcagc
     9661 cgcctcttta gtgtttaccg agcagcgttc agataaattt caattggtta caggcgttac
     9721 atggcacgct tcggcacggt acgcaaactg ctgctgcaaa tgccgtcaga tgagtagaca
     9781 ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat cgcctgcttc
     9841 gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc
     9901 accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgatcagg
     9961 attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg aatgtcacca
    10021 acggcaagcg tttctccacc tatgccatcg cggcagaacg cggttcgaga attatttctg
    10081 ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc gccagcttcg
    10141 ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat tttgaaggcg
    10201 acaacgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcctga ttgttatccc
    10261 tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct taagatataa
    10321 agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat ggtcgtcacc
    10381 gccaggtgaa atccggcttc ttttgctgcc ttcacggctt tgtcattaaa tccgccaaat
    10441 ggataagaaa gatacaaaac atgtggatta aattgcgcca gagcgcggcg tgaacgtgca
    10501 aaatcaaaca gaatattgtg ctcactacgg ctcagcaata taggccgccg ataaccatct
    10561 atccgatgca aaaaatgagt atgtgactgg aaatcaaata catcgcgaat ttcgttaagt
    10621 tcagaaacgc tcataaattg cagcgatttt gggttccatt tctgcgggtg acgtttgatg
    10681 cgcgaggtaa caataaacgc cgtcgccttc ataccatatt gtttcaacac aggatacgca
    10741 tagcggctca ccgacttgag gccatcatca aaggtaatta ccaccgctcg cgcagggaga
    10801 ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc gtatcccctg
    10861 tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg
    10921 cgaaaacggg tgttttcttc gtcgcgcaaa atatggtgat aggttagcac cggcaagcca
    10981 ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat gcggatctga
    11041 taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc
    11101 gccagtactc caaatggcgc actgcccaca ctcggcgcgt tataaaccgg cgtatctttc
    11161 caggttatta agttctgatt actcagcggc ttgttgaggt cacctaaacc atcttcaact
    11221 ttttgtcgcc cctgaaccgg ctcgagatgg cctttatcga taaacccttt accaaagcca
    11281 aaattaaata cgtaataact tgcggcagtg ggctccacgg caatgatttg tccggcccga
    11341 atattcccca ccgtcaccat cttgtcacca atttgcgccc agaccgcggc attttcgatg
    11401 gtttgcatat aatgggcagg taatgcggca ctgacgcttg cggtaaacag catcagcagg
    11461 agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg cttttattgt
    11521 agcaaaagcg tcatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa
    11581 ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcgcacc cagttcgaca
    11641 atatgctcgc gaaacgctga actggtcatg gtttcacggc aagccatcat ctgttcaatt
    11701 aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa
    11761 gccacgcggt acggtggcgc tccggctata tcggttaaaa agataacgcc ctcaccagag
    11821 tccgtttcat gcaaggcatc gcacatcatg cggctgagca tattagaggt taatccgcgc
    11881 cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag cgcatccagt
    11941 atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt
    12001 agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct
    12061 ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga
    12121 tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag
    12181 tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg atcatataaa
    12241 cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc agggagtaaa
    12301 acaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat
    12361 cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc accgcagtga
    12421 gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg
    12481 aaattgccgt caccaaaata ccaacaaaca gaccacgcgc cacgccacca ccgacatatc
    12541 cggcaataat gacatgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga
    12601 acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc accgacatca
    12661 tgatcagccc cggtacgatg aactgcatgt agctgaagcc atgcatatcg ccaatacgcg
    12721 aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact ggcggcacca
    12781 gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg
    12841 ccacccagta aagatgcatc atgtgcgatc tccttgtttt tcattaacca gtgaaacaaa
    12901 cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact
    12961 taactgcgta aatacgctgt tgatgccctg ctcacgcagc acttcaactt ccagcgtggc
    13021 ggtatctacc agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag
    13081 atcgagaata aaggtttctg atttcagctt cgccagcagc gccttcatcg aagtattttc
    13141 caccaactca ccgtgttgaa taatgccgat attgcggcac agcatttctg cttcttccag
    13201 gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca
    13261 cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa tcagtagttt
    13321 aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat
    13381 acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatact tttcgctgcg
    13441 aatgtacgct tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg
    13501 cacggtttca aacgggttga agttaaattc ctgtggcacc agacccaact gacgtttagc
    13561 gtttacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt
    13621 taccagagag ctgataatac cgatagtggt cgattttccg gccccgttag gcccgagaag
    13681 cgcataaaaa tcacccgctt cgacctgcaa atcgattcca cgaagcgcct gaacgccgcc
    13741 tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc
    13801 ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa
    13861 acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc
    13921 aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg
    13981 gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca
    14041 gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaacctg
    14101 gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa
    14161 gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc cgcagttgaa
    14221 aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa
    14281 catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg
    14341 aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa
    14401 cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt
    14461 gatctggatg tcacagcgac taaccgcgaa acccttgagc aacgttaccg tcacgggatc
    14521 tccaacctca agctgaagca catcaatcac aaataaattc gccatgccgg atgcaacaca
    14581 tccggcaact tcacacttaa tcgtccagca gaatcacttt gccgatatac ggcagatgac
    14641 ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac
    14701 cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa
    14761 tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc
    14821 ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct tcatccagat
    14881 ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca
    14941 taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg
    15001 agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa
    15061 tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta
    15121 cagtatgttt catatctata accatatgat ttaaaaacaa atcacttcgc acgggcaggt
    15181 ttcagcaccg caacaaagcg caagacacgc agtataccag taaaactatt cattcgcgga
    15241 atctgttaat aaagctcgaa ttgtgatgac gatcacacat gttaaaacct ataattaatt
    15301 gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac aataccggct
    15361 cgcggcgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac
    15421 tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc
    15481 ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctgt
    15541 acgcagccct gctgctcggc actatgattt ggggcgtctg ggaagttggt ttcgacttct
    15601 gggcgctgac tccgcgcagc gacattctgg tcttcttcgg catctggctg atcctgccgt
    15661 ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac
    15721 tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag gaaatcagcg
    15781 gcaccttaag cgccgatacc acgccagcag aagccatctc ccccgttgcc gatcaggact
    15841 ggcctgccta tggtcgcaat caggaaggtc aacgcttttc accactgaag caaattaacg
    15901 ctgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc
    15961 cgaacgatcc gggcgaaatc accaatgaag tgacgccaat taaagtgggc gacacccttt
    16021 acctgtgtac cgcgcatcaa cgtctgtttg cgctggatgc cgccagcggt aaagagaaat
    16081 ggcattacga tcctgagctg aaaaccaacg agtccttcca gcatgtaacc tgccgtggcg
    16141 tctcttatca tgaagccaaa gcagaaactg cttcgccgga agtgatggcg gattgcccgc
    16201 gtcgtatcat tcttccggtc aatgatggtc gtctgattgc aattaacgct gaaaacggca
    16261 agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccggaca
    16321 ccaaaccggg tctgtatgaa ccgacttcgc cgccgattat caccgataaa accatcgtga
    16381 tggccggttc agtcaccgac aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt
    16441 ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaatg
    16501 cgatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg gcaccagcgg
    16561 cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtcaccacg ccagatatct
    16621 ggggtggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg
    16681 ccactaccgg gaaactggcg tggagctatc agaccgttca ccacgacctg tgggatatgg
    16741 atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccggtta
    16801 tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat ggtgaactgg
    16861 tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgtgaccc
    16921 ctactcaacc cttctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata
    16981 tgtggggtgc caccatgttt gaccagttgg tgtgccgcgt gatgttccac cagatgcgct
    17041 atgaaggcat ttttacgccg ccatctgaac agggtacact ggtcttcccg ggtaatctgg
    17101 ggatgttcga atggggcggg atttccgttg atccgaatcg tgaagtggcg attgccaacc
    17161 caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccaggcaac ccgatggaac
    17221 agccgaaaga tgccaaaggc acgggtacgg aatccggcat ccagccgcag tacggtgtac
    17281 cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccatgt aaacaaccag
    17341 catggggcta tatctcagca ttggatctga aaaccaatga agtggtgtgg aagaaacgta
    17401 ttggtacgcc acaggacagt atgccattcc cgatgcctgt accggtgccg ttcaatatgg
    17461 gtatgccgat gcttggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta
    17521 cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc
    17581 gtttaccagc gggtggtcag gctacgccaa tgacctatga agtgaatggc aagcagtatg
    17641 tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg
    17701 cttatgcgtt gccggatgat gtgaagtaac cataatgccc ggagagatcc gggcatattt
    17761 ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag
    17821 cagatggcag tgcgccatat aagcatgttc tttcggtgca tcgtgattaa acttcaccag
    17881 cacttcgctg acattacctt ctaccttaac ggtatctttc cagcctgcgc gatgcgtcgc
    17941 tggcggtttg ccattttctg acaagatacg gaactgcgta ccgtggatat ggaacggatg
    18001 cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc
    18061 aaacatcggc ttattcatat caaacgcctg accgttgatt ttgttggcat ggtggaaatc
    18121 aaacttcccg ctgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg
    18181 gctgtgatcc atcccggcca tcgcctgatc gccatatttc tccatcagca tctgcatccc
    18241 catcatatcg agcattgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag
    18301 cgaaggtaac gcaggcaggc tacttaatgt gtctggcaaa gcaccagagg cactaatggc
    18361 aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgtaatcg ccatccccat
    18421 ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac
    18481 ttcaaaacgc tcgcccatca gcactggcag ctcgttcacc ttcaccggtt caggtagcag
    18541 accgccgtcg ctggcaatca cataaagcgg gcgattgtcg ctggtggcga aattgagcga
    18601 gcgggcatta cagccattga gcaaacgcag gcgcagccag ccacgcgggg cagcgtgttg
    18661 cgggtagatt gcaccgttgg tcagcaacgt atcgccaaac caacccacag cggcggtcat
    18721 cacatccagt tgataatcaa tctgcccgtc agcgttaaat tttttatcct gaacaatcac
    18781 cgggacatca tcaatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc
    18841 aatcacaacc aaaccagcca gccccatcgc cacctgtcgc ccggtcttgc catgttgatg
    18901 tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc
    18961 acctgacgga ataattccct gcgggccgcc gtccacttca cccggtactt ccagcccgtg
    19021 ccagtgcaac gtcgtctctt ccgtcagttg gttgtagata tcaaccgtta ccgctttgcc
    19081 acgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcggt
    19141 tttcccgcca aaggtggact gacctgcgcc aatagttaac tgaatgcgat tacgggcatc
    19201 ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaata ctgcgcggct
    19261 ccacagcggc aaggctgaag ccacacccag cgcgacggaa tatttcaaga aatcacgacg
    19321 ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc
    19381 cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc
    19441 ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg
    19501 tttttcagaa cagtgttatt cggcagcctg atggccgttt gcgcaaacag ttacgcgctc
    19561 agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtttt tctgaagaac
    19621 gattgtggtt accagaactt acctaacggg caaattcgtc gcgcgctggt ctttttcgcc
    19681 cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc cctcggtgaa
    19741 gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg caaagccctg
    19801 gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga
    19861 aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccatt tttttacggg
    19921 tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga aacgctacac
    19981 gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa gaccgatcac
    20041 caggatctga tcatttttga gaacgccgca tttggtcgcg taatggcgct ggatggtgta
    20101 gtacaaacca ccgagcgtga cgagtttatc tatcatgaga tgatgaccca tgttccgcta
    20161 ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg tgccatgctg
    20221 cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat cgatgcgggt
    20281 gtcgtgtcgt tctgccgtca gtatcttccc aaccataacg ccggtagtta cgacgatccg
    20341 cgctttaaac tggtgatcga cgatggtgtc aatttcgtta atcaaaccag ccagaccttt
    20401 gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct gtttacttct
    20461 gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac
    20521 ggcgtctgct ttttacagca ggaagaggcc atcgacagcc atcgcaaact cagccattac
    20581 ttcagcgatg ttggctttta tcaggcggcg atcccgacct attacggcgg catcatgacc
    20641 tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat tattcaggcg
    20701 cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcagtcca tacggcagct
    20761 tttgccttac cccagtatct gcaagatgca ctggcttcac agccgtccta aggagaagat
    20821 aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag tctgagtttt
    20881 tgtatttacg atatctgcta cgccaaaact accgaagagc gcgacggtta tattgcttat
    20941 atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac ctgttccatt
    21001 atcggggcca atattcttaa catcgcccgc caggattacg agccacaagg tgccagcgtc
    21061 actattctgg tgagtgaaga accggtcgac ccgaaactta tcgacaaaac agaacacccc
    21121 ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg cgtacatacc
    21181 tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat tgaagtctct
    21241 acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca gcttgagtcc
    21301 gatattgtga ctattgatta tcgcgtgcgc ggttttaccc gcgacattaa cggtatgaag
    21361 cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga tatgaaggcg
    21421 ctgtatgaca tggtggatgt aaacgtctat caggaaaata tcttccatac caagatgttg
    21481 cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga tttaaccgac
    21541 agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg
    21601 cgcaatatgc ccgctgttta acgtctcagg cggagcacgg aggctccgcc tgatttactt
    21661 acttctgctg cacgaaatta cggtaagccg caaccacctg aagaaaatcc tcaacgccgc
    21721 acagcgacaa gctctcttca tcgtagtagt tcatcccctc ttccatttca tcgccagcga
    21781 attccagttg attggcgcga accatcacct cttcaccgtc catccacaga gtgtattcat
    21841 gccctgcccg ctgccaggat cgttcgctgc ctttgagcgt gcgcgctgcg tcttccactt
    21901 catcaagcaa ggccaggttt tctttcacct cttcattaaa ccagtgcccg accacttcat
    21961 gccccatgga catgcgcacc tttaccactc cggtaatatc gcgcagaaat tcgtaatcca
    22021 tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgccatt gatgtaatta
    22081 tcgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa aaaatgcccg
    22141 ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaaa
    22201 ccgcagtctg gaaaatcacc ccgtccgctt tttcggtgta ctgagaaagc tggtcgaagt
    22261 tcagataacg gtaagtatca acggctgttt tatctacctg cgccacgtag gtctggtact
    22321 cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc agccagttcc gcagaagcca
    22381 ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca
    22441 ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag ccagggatct
    22501 cgatacgagc accactctta ccgaagacgc tgtagtagcc ttcttcggtc agctgcgcag
    22561 cgtccatacg cgttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat
    22621 ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata
    22681 cttcgtcaat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc gggtcattcg
    22741 gtgcacagag gattggctct ttgatatccg ccagatcgat gtcgatcact gccgcgtatt
    22801 ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa
    22861 tacgacgctc cagggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga
    22921 cgatgttaga gttcaggtat tcaatgatcg gttctttgtt cagcttgatg gtacaaccgg
    22981 cagcagaacg ctcggcagac gcatctgtca gctcaaatgc ctgctcaact ttcagatccg
    23041 gcagaccttc aatttccaga atgcggccag agaagatgtt tttcttacct ttcttctcaa
    23101 cggtcagcag accttgtttg attgcataca gcggaatggc atgtaccaga tcgcgcaggg
    23161 tgatacccgg ctgcattttg cctttgaagc gtaccagaac ggattctggc atatccagcg
    23221 gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg aaagagatac
    23281 cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac
    23341 ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacaccg ccacggttca
    23401 taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg
    23461 tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca
    23521 gttcatcacg ggtcatcggg ccggtggtat cctgagaacc aacagaagtc attttcggtt
    23581 cacagtacgc gcccggacga atgcctttca cgccacaggc acggcccacc attttctgcg
    23641 ccagcgagaa gccacggtcg ctctcagcga catctttcgc ctgacggaac acatcgctgt
    23701 gcggcaggcc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggaa
    23761 tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggttgcca
    23821 gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaattacgt
    23881 cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgca tcttccatcg
    23941 tgttaaaaaa gattggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg
    24001 gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg
    24061 atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag
    24121 cttcaatctg tttgatcggg ccaacaacac caggctgatc tggctcaata ccttcacggg
    24181 cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatctggtg
    24241 ccggagagag gtcatcggtg ttggtttcgc cagtgacttt gaagacggta acggtcagtt
    24301 tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct
    24361 gcttcgcata ttcattgcct gctttggctt tctcttctac gtcatagaag ttatcgaaca
    24421 tcagcagcgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca tcatccagcg
    24481 cgtcgatcag cggatgaatg ttgtaaccac cctgcatagt gcccagcagt tcaatggctt
    24541 tttccggagt cagcagcggg gatttggctt cgcctttcgc gacagcagcc aggaagcctg
    24601 ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaac agatctaaca
    24661 ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg gccatttggt
    24721 ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt
    24781 attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct
    24841 tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat
    24901 agcaggattt tgacgatgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga
    24961 atcgttaaag atagtctaga gaatgtttcc ctccgtaaaa acttcgttaa ttcgcatggt
    25021 gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac ggcctcttta
    25081 tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat
    25141 ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc
    25201 cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt
    25261 ggaagcgcaa tcgacacaac agactgcgcc tgacatctcc gcagttacgc ttcctgcaac
    25321 ggtttccgcg cctcccgtaa cgcctgccgt cgtcaaatcc gcattcagca ctgctcaaat
    25381 agatcaatgg gttgcacctg tcgcgctata ccccgactct ctgctttcac aagtgttaat
    25441 ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg ataatccact
    25501 taaacaaggc gatgctgcta ttcaggctgt atctgaccag ccctgggacg ccagcgtcaa
    25561 atcactggtg gcctttccac agttgatggc attgatgggc gaaaacccgc aatgggtgca
    25621 aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt
    25681 gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca actgaacaaa agatcatcgc
    25741 gacaacgaaa aaagtcgtac cggtaaatca gcccgccaac gcccctgcca cacaatcaaa
    25801 taccgtttct acgtccagcc ccgtcgtcgc agaacctgca ccgaccgtaa taaccattga
    25861 gccagccaat ccggatgtgg tttatattcc gaactacaac ccaaacgtgg tttacggcag
    25921 ttgggccaat accgcttatc cgccagttta tctgccgccg ccagccggag aaccgtttgt
    25981 tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctactacat acgcactatt
    26041 cagcagcatc gactgggacg acgacgatca tgaccatcat catcatgacg atgatgatta
    26101 tcatcaccac gatggcggtc atcgtgacgg taatgactgg caacacaacg gcgacaacat
    26161 caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat
    26221 ggcatggcgg cacaatccaa actaccgtaa tggtgtgacc tatcatgatc aggatatggc
    26281 aaagcggttt catcaaaccg atgtcaacgg cggaatgagc gccacgcagt tacctgctcc
    26341 aacacgcgac agccagcgtc atgcggcagc aagtcagttt cagcaacgaa cacacgcagc
    26401 accagtcact acgcgagata cccagcgtca ggctgcggca cagcggttta atgaagcgga
    26461 acactatggc aattatgacg acttccgcga cttcagccac caccaaccac tgacccagca
    26521 gcaaaaggac gccgctcgtc agcgttatca gtcggcttct cctgagcagc gccaggcagt
    26581 ccgcgagaaa attcaggcta acccgcagaa tcagcagaga agagaagcgg cacgtcagcg
    26641 tattcagtcc gcgtcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc
    26701 gcagaatcaa cagcaaagag atgcagcacg tcagcgtatc cagtctgcct cgcctgagca
    26761 gcgtcaggtt tttcgagaga aagttcagga gagccgcccg cagcgtctaa acgacagtaa
    26821 ccatactgcc cgattgaata atgagcaacg gtcagcggta cgcgaacggt tgtctgagcg
    26881 tggagcaagg agactggaaa ggtaaattgc aggcgtaaaa aagcggcgtt gttagccgct
    26941 taagactgat gacaaacatc gaatcgcccg atgcgcaagc ttatcgggcc tacacaagcc
    27001 atgcaatata ttgaatttgc atgattttgt aggtcggata aggcgtttac gccgcatccg
    27061 gcatcaacca cgagcacttt atcaacaatc tgaagcggcc agaatagccg cttcttaatt
    27121 tgcaggatgt tccggcaaac gaaaaattac ttcttcttcg ctttcgggtt cggcaggtcg
    27181 gtaatgctac cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc
    27241 gcatggatgg tcagtgcgat gtcttcagcg tcacaaccca tttcgattgc caggccgatt
    27301 tcacccagca gctcaccgcc gttagtaccg acaattgcac caccgatcac acggtgagat
    27361 tctttgtcga aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca
    27421 gaagcagccc acgggaaggt ggcggtttcg tagctgatgc ctttctcttt cgcttctttc
    27481 tcagtcagac ctacccatgc aacttctggt tcggtatagg cgatggacgg gataactttc
    27541 ggatcgaagt aatgtttttt accggcgata acttcagcgg caacgtgacc ttcgtgaaca
    27601 cctttgtgtg ccagcattgg ctgaccgacg atatcaccga tagcaaagat gtgcggtacg
    27661 ttggtacgca gctgtttgtc aacgcggatg aaaccacggt cgtcaacttc cacgccagct
    27721 ttgcctgcgt cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg
    27781 taacgctgcg gttcagcggg tgcttttttg ccttccatcg tcacataaat gccgtcttct
    27841 ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc
    27901 ttggtaaaga ctttaacgat gtctttatca gctgccggga tgacctggtc gaacatttca
    27961 accacgtcaa tctgtgaacc cagcgcgtgg tatacggtgc ccatttccag accgatgata
    28021 ccgccaccca ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag
    28081 tcccagatac gcggatcttc atgcggaata aacggcagtt gaatcgggcg agaacccgct
    28141 gcaatgatcg cgttgtcgaa gttgatcacg gttttaccgt tctcaccttc aacttccagg
    28201 gtgttagccc cggtaaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc
    28261 ataccagcca gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg
    28321 tcgatatcgg ttttcggttc gccaaagacg ataccgtgtt cagccagcgc tttggcttct
    28381 tcgataactt ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa
    28441 acaccgccga gggtgttgta gcgttctacg attacggttt ccagacctaa atcagcgcaa
    28501 cggaaggcag cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt
    28561 tcagtactca tcatgacctc tatatattta tctccggcgg gtctgacgtt ttttttatga
    28621 cgcccattat ccaccgggtc gttctatccg tcggtatttt acaaaattgt taataatttt
    28681 taaacaacaa acggcaaccg atttgtctat tcgctaataa tctcaataca ttcatgagat
    28741 taccagaaaa gagccggccg ttgggccggc tcttttattt acatcaccag acggcgaatg
    28801 tcagacagcg tgttgttaat gatggtaatg aaacgggcac catcagcacc gtcgatcacg
    28861 cggtggtcga aggagagaga aatcggcagc atcagacgcg gcacgaactc tttaccattc
    28921 cacaccggct ccatcgcgga cttggaaacg ccgaggatag ccacttccgg cgcgttcaca
    28981 atcggcgcga agtgggtagt acccaggccg ccgatgctgg agatggtgaa gcaaccgccc
    29041 tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct tagaaatagt catcagctcg
    29101 cgagacagct cgatgatgcc tttcttgttg acgtctttga ataccgggac aaccagaccg
    29161 ttcggggtat ccaccgccac accgatgttg atgtatttct tcagggtcag acgctgaccg
    29221 tcttccgaca gcgaactgtt gaagcgaggc atctgctcaa gagctgcagc aacggctttc
    29281 atgatgaaga caaccggggt gatcttcaca tccagcttac gtttcgccgc ttcttcgttc
    29341 tgctgtttac ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg agtaacatgc
    29401 gggatcatca cccagttacg gctcaggttc gcaccagaga ttttctggat gcggcccagt
    29461 tccacttctt cgatttcacc aaacttgctg aagtccacct tcggccacgg cagcatgcct
    29521 gggataccgc cgccagttgc cgccggagct gcttctgcac gtttgatagc ttctttcacg
    29581 taagcctgaa cgtcttcgcg caggatacga cctttacggc cggtgccctt cactttcgcc
    29641 aggttaacgc caaactcgcg tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg
    29701 tcattttcag caaattcaga tttgccttcg gcttttgcag ccggagctgc tgccggggct
    29761 tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc tgccgcaggc
    29821 gctgcgcctt caacttcgaa gatcataatc agcgaaccag ttttcacttt atcgccaacg
    29881 ttgactttca gttccttcac gacgcctgca aacggagccg gaacttccat agaagcttta
    29941 tcgccttcta cggtgatcag tgactgttca gcggcaactt tgtcgcccac tttcaccatc
    30001 acttcggtca cttcaacttc gtcaccgccg atatccggaa cgttaacttc tttcacgcca
    30061 gctgctggtg caggggccgc tgccggagcc gcttcctgtt tagccgccgg agctgccgcg
    30121 cctgcttcac ccgcgacttc gaagatcata atcagcgagc cggtagacac tttgtcaccc
    30181 acgttcactt tgatctcttt cacggtgcct gcaaacggag caggaacttc catagaagcc
    30241 ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc aactttcacc
    30301 aggatttcgg tcacttcaac ttcgtcgctg ccaatatccg gaacgttaac gtcttttgct
    30361 gccgcagccg ctggtgctgc tgccggagct gcttctttct tctcttctgc ctgagcaggt
    30421 gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt
    30481 ttatcgccaa cagagacttt gatctcttta acgatacccg cctgcggaga tggtacttcc
    30541 atagaggctt tgtcgccttc tacggtgatc agcgactgtt cggcttcaac tttgtcgccc
    30601 actttgacca ggatctcggt gatttcaact tcatcagccc cgatgtccgg tactttgatt
    30661 tcgatagcca ttattctttt acctcttacg ccagacgcgg gttaacttta tctgcatcga
    30721 tgttgaattt ggcgattgcg tcagcaacca ctttcttatc gatttcgcca cgtttagcca
    30781 gttcgcccaa cgccgcaacc acgacataag aagcatcaac ttcaaagtgg tgacgcaggt
    30841 tctcacggct gtcggaacga ccgaagccat cagtacccag tacgcggtag tcgtcagccg
    30901 gtacgtaagt acggacctgc tcagcgaaca gtttcatata gtcggtagat gccactgccg
    30961 gagcgtcgtt catcacctga gcgatatacg gaacgcgcgg agtttccagc gggtgcagca
    31021 tgttccagcg ttcacaatcc tgaccatcac gcgccagttc ggtgaaggaa gtcacgctat
    31081 aaacgtcaga acctacgccg taatctttcg ccaggatctc tgccgcttca cggacgtgac
    31141 gcaggataga accggagccc agcagctgaa ctttaccttt gctaccttca atggtttcga
    31201 gtttgtagat acctttacgg ataccttcct cagcaccttc cggcattgcc ggcatgtggt
    31261 agttttcgtt cagcgtagtg atgtagtagt aaacgttctc ttgtttttca ccatacatac
    31321 gctccagacc gtcatgcatg atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga
    31381 tacagttcgg gatagtcagc gactgaatat ggctgtgacc atcttcgtgc tgcagacctt
    31441 cgccgttcag ggtggtacga ccggaagtac cgccgatcag gaagccacgc gcttgctggt
    31501 cgccagccgc ccagcacaga tcgccgatac gctggaaacc gaacatcgag taatagatgt
    31561 agaacgggat catcggcaga ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac
    31621 aacctgcgcc cagctcgttg atcccttcct gcagaatctg acctttctcg tcttctttat
    31681 agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg ctgtaaatac
    31741 caatctgacg gaacagacct tccataccga aagtacgcgc ttcgtcggcg atgatcggta
    31801 ccagcctgtc tttgatcgac ttgttcttca gcattacgtt cagggcacga acgaaagcga
    31861 tagtggtaga gatctctttg ctctgctctt ccagcagcgc gccgaagtct tgcaggctcg
    31921 gcagctcaag cttctcggtg aagttcggct gacggcttgg cagataaccg tgcagtttct
    31981 gacgctgagc gtgcagatag gtatgctctt cagaaccttc cgggaaggtg atgtacggca
    32041 gtttttcgat atctgcatca gacaccggca cattgaaacg gtcgcggata tgacgcacgc
    32101 cgtccatgtt cattttctta acctggtgcg cgatgttttt accttcagcc gcgtcgccca
    32161 tgccataacc tttaatggta tgagcaagga ttaccgtcgc tttgcctttg gtttcctgcg
    32221 ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc accacggttc agtgcccaga
    32281 tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc aggatattta ccgaagaagt
    32341 gttcgcgaac atacgcacca tctttcgatt tgaaggtctg gtaatcgccg tcaacggttt
    32401 cgttcatcag ctggatcagt ttaccgctgg tatctttacg cagcagttcg tcccaacggc
    32461 taccccacat cactttgatc acgttccagc cagcaccttc gaagatgcct tccagttcgt
    32521 tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa
    32581 cgaagaccag attatccagt ttttcacggg tagcgatggt gatcgcacct ttggattccg
    32641 gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt ttgtttagag gtatctttca
    32701 ggccacggtg ttccagatat ttcaggaatt tagcctggta aatagcacca atcggaccca
    32761 gacccataga tacggtcggg aactgccaga attccggcat cagtttcggg tgcggatagg
    32821 aagagaggcc attgccgtga acttcctgac ggaagttatc cagctgctcc tgagtcagac
    32881 gaccttccag gaaagcacga gcgtacacgc ccggggagat gtggccctgg aagtaaacca
    32941 ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa
    33001 tggttgcgga agactggaag gacgccatgt ggccgcccag ttcgaggtct tttttcgacg
    33061 cacgcagcac cgtcatgatg gcgttccagc ggatagctga acgaatacgg cgttccagtt
    33121 ccagattacc cggatactcc ggttgttctt caacggggat ggtgttgatg tagttgctga
    33181 taccggtgcc tgcagctacg ttgactccgc ctttgcgggc ttcagcaagc agttggtcga
    33241 tcagatactg agcacgctca acaccttctt cacggatgac cgattcgatc gcctggagcc
    33301 agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg ggttattcct
    33361 tatctatcta ataacgttga gttttctgga acctgtccca ttgaactctc gccggaaagc
    33421 tcaataagac aggttctacg tttagttgcc gcgcttttat atgcgcttga tttacaacat
    33481 cttctggata atttttacca gaaaaatcac taattctttc gttgctccag acgacgcaga
    33541 gaacgctcac ggcggctctc ttcacggctt ctgtcgagca aaatttcttc gataaaggcc
    33601 agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat cgcttcaaat
    33661 atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata gagcaattcg
    33721 aagttctggc ggacattctg agccaacatc ggctccatac accttagcag atgaagcaga
    33781 accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc atttgattcc
    33841 gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc acggatgcgt
    33901 tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc ttccagggcg
    33961 tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt cggagagcag ctccaccagc
    34021 ggatcgctga agctttgcca taggctgctc tggacaaaag tgccgccacc ctggcgacga
    34081 agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaatgaggg acgggagacg
    34141 tcaaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg gagagtgcct
    34201 tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg ttggcggatt
    34261 ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa
    34321 tttcatgtct gtgacgctaa agtaacaaag tattcacctt atgtccatac aggttttgat
    34381 tgaaatcatg aaactgtgca cattttaaca acttgacata tataacgttt caaagttgta
    34441 actatgcaca aatgttaaat ttacccatca aggggtgacc ttaacagaat tgaaacgaaa
    34501 aaatatttcc tctgaaccga ttcaccaagc aacaaaacgc ataacaattc aattatgtga
    34561 attaatatgt aaaatgaatg ttcagggaag gagagaaagg tagtatttac ctatgttttc
    34621 tttttacatt gcttattatc actgcaatcg ccaccgtaac cttctcgtag cgaaaatcat
    34681 cacttaacca gaacgtgatg attttcaggt gacagattct gtttagagtt cattctgaat
    34741 ctttgaacaa aggcaatcct tgcgaacgcc gccattcttt gacgattttc aggatgtttt
    34801 caggtttacc atcttctggg gaattaggat agaaaatgag atctcccttt ttaggatgtt
    34861 cagtaatttt aatgaaatga tccactaagg tatcgagata tttttctaat tttttccctt
    34921 tta
//
LOCUS       DADTFG010000035        34892 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.035, whole genome shotgun sequence.
ACCESSION   DADTFG010000035 DADTFG010000000
VERSION     DADTFG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.035"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            58..1713
                     /locus_tag="J1138_001577"
     CDS             58..1713
                     /locus_tag="J1138_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HBA8453391.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATVLTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPARSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1804..3039)
                     /locus_tag="J1138_001578"
                     /pseudo
     CDS             complement(1804..3039)
                     /locus_tag="J1138_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(3186..3262)
                     /locus_tag="J1138_001579"
     tRNA            complement(3186..3262)
                     /locus_tag="J1138_001579"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3226..3228),aa:Pro,seq:tgg)
     gene            complement(3305..3391)
                     /locus_tag="J1138_001580"
     tRNA            complement(3305..3391)
                     /locus_tag="J1138_001580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Leu,seq:cag)
     gene            complement(3412..3487)
                     /locus_tag="J1138_001581"
     tRNA            complement(3412..3487)
                     /locus_tag="J1138_001581"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3452..3454),aa:His,seq:gtg)
     gene            complement(3546..3622)
                     /locus_tag="J1138_001582"
     tRNA            complement(3546..3622)
                     /locus_tag="J1138_001582"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3586..3588),aa:Arg,seq:ccg)
     gene            complement(3725..5110)
                     /locus_tag="J1138_001583"
     CDS             complement(3725..5110)
                     /locus_tag="J1138_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA8453392.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5301..6041)
                     /gene="rffM"
                     /locus_tag="J1138_001584"
     CDS             complement(5301..6041)
                     /gene="rffM"
                     /locus_tag="J1138_001584"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBA8453393.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFECIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6044..7396)
                     /gene="wzyE"
                     /locus_tag="J1138_001585"
     CDS             complement(6044..7396)
                     /gene="wzyE"
                     /locus_tag="J1138_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HBA8453394.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7393..8472)
                     /gene="rffT"
                     /locus_tag="J1138_001586"
                     /gene_synonym="wecF"
     CDS             complement(7393..8472)
                     /gene="rffT"
                     /locus_tag="J1138_001586"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBA8453395.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8469..9719)
                     /gene="wzxE"
                     /locus_tag="J1138_001587"
     CDS             complement(8469..9719)
                     /gene="wzxE"
                     /locus_tag="J1138_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBA8453396.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFANWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9721..10851)
                     /gene="rffA"
                     /locus_tag="J1138_001588"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9721..10851)
                     /gene="rffA"
                     /locus_tag="J1138_001588"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBA8453397.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGR
                     IELPSIPDGCVQNAHMFYIKLRDIADRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10856..11530)
                     /locus_tag="J1138_001589"
     CDS             complement(10856..11530)
                     /locus_tag="J1138_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-fucosamine acetyltransferase"
                     /protein_id="HBA8453398.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11508..12389)
                     /gene="rfbA"
                     /locus_tag="J1138_001590"
     CDS             complement(11508..12389)
                     /gene="rfbA"
                     /locus_tag="J1138_001590"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBA8453399.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQSKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12408..13475)
                     /gene="rffG"
                     /locus_tag="J1138_001591"
     CDS             complement(12408..13475)
                     /gene="rffG"
                     /locus_tag="J1138_001591"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBA8453400.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLEVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLNG"
     gene            complement(13475..14737)
                     /gene="wecC"
                     /locus_tag="J1138_001592"
     CDS             complement(13475..14737)
                     /gene="wecC"
                     /locus_tag="J1138_001592"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBA8453401.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14734..15864)
                     /gene="wecB"
                     /locus_tag="J1138_001593"
     CDS             complement(14734..15864)
                     /gene="wecB"
                     /locus_tag="J1138_001593"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBA8453402.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15920..16966)
                     /gene="wzzE"
                     /locus_tag="J1138_001594"
     CDS             complement(15920..16966)
                     /gene="wzzE"
                     /locus_tag="J1138_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBA8453403.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKSQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(16978..18081)
                     /gene="wecA"
                     /locus_tag="J1138_001595"
     CDS             complement(16978..18081)
                     /gene="wecA"
                     /locus_tag="J1138_001595"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBA8453404.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18321..19580)
                     /gene="rho"
                     /locus_tag="J1138_001596"
     CDS             complement(18321..19580)
                     /gene="rho"
                     /locus_tag="J1138_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBA8453405.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19665..19766)
                     /gene="rhoL"
                     /locus_tag="J1138_001597"
     CDS             complement(19665..19766)
                     /gene="rhoL"
                     /locus_tag="J1138_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HBA8453406.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19907..20236)
                     /gene="trxA"
                     /locus_tag="J1138_001598"
     CDS             complement(19907..20236)
                     /gene="trxA"
                     /locus_tag="J1138_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBA8453407.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20367..21632
                     /gene="rhlB"
                     /locus_tag="J1138_001599"
     CDS             20367..21632
                     /gene="rhlB"
                     /locus_tag="J1138_001599"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBA8453408.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21768..23252
                     /gene="gppA"
                     /locus_tag="J1138_001600"
     CDS             21768..23252
                     /gene="gppA"
                     /locus_tag="J1138_001600"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBA8453409.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23299..25320)
                     /gene="rep"
                     /locus_tag="J1138_001601"
     CDS             complement(23299..25320)
                     /gene="rep"
                     /locus_tag="J1138_001601"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBA8453410.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVAQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     SNFSLFDDTDQVALLKELTDGLIEDDKLILQQLISTISNWKNDLMTPVQAAASAKGER
                     DRIFAHCYGLYDAHMKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGKRARFTVVGDDDQSIYSWRGARPQNLVLLSKDFPALQVVKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25405..25686
                     /gene="ppiC"
                     /locus_tag="J1138_001602"
     CDS             25405..25686
                     /gene="ppiC"
                     /locus_tag="J1138_001602"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBA8453411.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFSKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25732..27207)
                     /gene="ilvC"
                     /locus_tag="J1138_001603"
     CDS             complement(25732..27207)
                     /gene="ilvC"
                     /locus_tag="J1138_001603"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBA8453412.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27357..28247
                     /gene="ilvY"
                     /locus_tag="J1138_001604"
     CDS             27357..28247
                     /gene="ilvY"
                     /locus_tag="J1138_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBA8453413.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28244..29788)
                     /gene="ilvA"
                     /locus_tag="J1138_001605"
     CDS             complement(28244..29788)
                     /gene="ilvA"
                     /locus_tag="J1138_001605"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBA8453414.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29791..31641)
                     /gene="ilvD"
                     /locus_tag="J1138_001606"
     CDS             complement(29791..31641)
                     /gene="ilvD"
                     /locus_tag="J1138_001606"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBA8453415.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSAQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31706..32635)
                     /gene="ilvE"
                     /locus_tag="J1138_001607"
     CDS             complement(31706..32635)
                     /gene="ilvE"
                     /locus_tag="J1138_001607"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBA8453416.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32655..32918)
                     /gene="ilvM"
                     /locus_tag="J1138_001608"
     CDS             complement(32655..32918)
                     /gene="ilvM"
                     /locus_tag="J1138_001608"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBA8453417.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32915..34561)
                     /locus_tag="J1138_001609"
     CDS             complement(32915..34561)
                     /locus_tag="J1138_001609"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBA8453418.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNTEMLEKLS"
     gene            complement(34564..34614)
                     /locus_tag="J1138_001610"
     CDS             complement(34564..34614)
                     /locus_tag="J1138_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453419.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34701..34799)
                     /gene="ilvL"
                     /locus_tag="J1138_001611"
     CDS             complement(34701..34799)
                     /gene="ilvL"
                     /locus_tag="J1138_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBA8453420.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8450 a         9638 c         8838 g         7966 t
ORIGIN      
        1 aattatcaat gcttaattac gtcatcattt tgacgacatg aaaccggagc attttgcatg
       61 gaattttcgt tttcacccaa acttcttgtt gttgctgtcg ccgcagctct tcctctcata
      121 gccagcgcag cagatacccc gtcaaccgcc accgcacgca aaggctttgc cggatacgat
      181 cacccaaacc agtatctggt taaaccggcg accactattg ccgacaacat gatgccggtg
      241 atgcagcatc ctgcacaaga caaagaaacc cagcagaaac tggcggaact tgagaaaaaa
      301 accggtaaga aaccgaatgt ggttgttttc ttgctggatg atgtgggctg gatggacgtt
      361 ggctttaacg gcggcggcgt ggcggtgggt aaccctacac cagatatcga cgccgttgcc
      421 agccaggggc tgattttaac ttcggcgtat tctcaaccga gttcttcccc aacccgcgcc
      481 acggttctca ccggacaata ctccatccac cacggcattc tgatgccgcc aatgtacggg
      541 caaccgggcg gtctgcaagg gttaaccacg ctgccgcagt tgctgcacga tcagggctac
      601 gtcacccagg ctatcgggaa atggcatatg ggggaaaaca aagagtcgca gccgcagaac
      661 gttggctttg atgatttccg cggctttaac tcggtgtccg atatgtatac cgaatggcgt
      721 gatgttcatg tcaacccgga agtcgccctg agcccggctc gttctgaata catccaaaaa
      781 ctgccgttca gcaaagatga cgttcatgcc gtgcgcggcg gtgaacagga agccattgcc
      841 gacattacgc cgaaatatat ggaagacctg gatcaacgct ggatggaata tggcgttaag
      901 ttcctcgaca agatggcgaa gagtgataag cctttcttcc tctattacgg cactcgcggc
      961 tgtcacttcg ataactaccc gaacgccaaa tatgcgggta gctctccggc gcgcacctct
     1021 tacggcgact gcatggtgga gatgaacgat atcttcgcca atctgtataa agcactggag
     1081 aaaaacggtc agcttgataa cacgctgatt gtgttcacct ctgataacgg tccggaagcc
     1141 gaagttccgc cgcacggtcg cacgccgttc cgcggtgcga aaggttctac atgggaaggc
     1201 ggcgttcgtg tgccaacctt cgtttactgg aaaggcatga tccaaccgcg taaatctgac
     1261 ggcattgtcg atctggcgga tctcttccct acggcgctgg atctggcggg gcatcctggg
     1321 gcgaaagtag cgaatttagt accgaaaacc acctttatcg atggtgtgga ccagacatct
     1381 ttcttcctgg gaacaaatgg tcagtctaac cgtaaggccg agcattactt cctcaacggt
     1441 aaactttctg ctgtgcgtat ggatgagttc aagtatcacg tcctgattca gcaaccttac
     1501 gcttataccc agagcggata tcagggtgga ttcaccggca cagtaatgca aacggcggga
     1561 tcgtcggtgt ttaacctgta caccgatccg caggaaagcg actctatcgg cgtgcgccat
     1621 atcccaatgg gtgtaccgct acagaccgaa atgcatgcgt atatggagat cctgaaaaaa
     1681 tatccaccac gcgcgcagat taaatctgat taagccggcg ctatcgctga gatctacctt
     1741 tgccggatgc gatgctgacg catccgacaa tcaaccgcag gcggccacaa aatggctgct
     1801 tttttactta ttcaccacca gcaaatgcgc ctgcataatg tcgctggccg gacgaccgtg
     1861 cgccagcaaa tcaaccattg ctttaagata tggcggtaga tggcggaaat aacgctgata
     1921 cccggcacac aaataattca acccgggttt gccgctggca tcgagcataa agcggtgttt
     1981 cgggcagcct ccccagcacg cttttaacac gttgcaactg cgacactgtg ccggtaactg
     2041 cttgaattta tcttcaccaa atgcctgctg ttgcggggaa tcaatcattt ctgcaatcgt
     2101 ctgctgaagc atattcccta gtcgatactg cggataaaca tagtgatcgc aggcataaac
     2161 gtcaccgttg tgctcaacaa tcaccgagcg cccacaggtt ggctgatgat ggcaaaccgc
     2221 gcccggtgcg ccgacaaaat tggcgaacgt ccattcgata ttcattacga aaatcttacc
     2281 gacgtcgcgc ttaatccagt ggtcgaaaat cgccaccaga aactcaccga actcctcggg
     2341 gtgtaccgac cattccgtca gttcgccctg aatatcgccg ggagcatgta gcttaagtcc
     2401 agcatgagca gccgtttcat cagccaggcg ctcgaccacc ggaataaact ggatgaactc
     2461 aacacctgca tcacacagaa aatcatagac ttgcaaaggt tgtaacgcac tggtgcgatt
     2521 gacgcagacc agcacgttat agtcgacatg atgtttttgc aggagcgtca gagcacgcat
     2581 caccagctta tgcgttggtc tgccaccttt ggtcacgcga tattgattgt ggatctcagc
     2641 cggaccatcc agcgataacc ctacaagaaa atgattttct gccagaaatg cacaccattc
     2701 gtcatcgagc agcacgccgt tagtctggaa gctattactt atcttcctgc cagcaccata
     2761 tttcgcctgt agcgccacgg cacggcggta aaaatccagc ccgagtaacg ttggttcgcc
     2821 gccctgctag gtaaaagcca cttcgttttg ggtttcgctg gcagcgatat agtgacggac
     2881 atacgcttcc agcgtgtcat cgtccatatg cgtgactggc ttttcgtggt aaagggattg
     2941 tttttcgaga taaaaacagt agtcacagtt cagattacaa tcagaaccgc tcggtttcgc
     3001 catcacatga aaagcacgcg ttggaacctg ttgctgcatg gtcgctcctt gcctgaagtc
     3061 atccttcaat aagttaaggg cgaaatcata ccgtggcgca atgcgttatt acgggcgttt
     3121 attgcgaagg gaaagtgacg aaatgcacag aaaacaaaaa accccgccga agcggggttc
     3181 aaaattggtc ggcgagagag gattcgaacc tccgacccac tggtcccaaa ccagttgcgc
     3241 taccaagctg cgctactcgc cgaaatactg ctttttgaat ttttagttca attctttaaa
     3301 gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag
     3361 ctagcgcgtc taccaattcc gccaccttcg cattgtcaca acttctaata atggggtggc
     3421 taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc aactgagcta
     3481 tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca agcgccggga
     3541 ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag ggcagcgctc
     3601 tatccagctg agctacgggc gcttagcgcc gttgcggggg tggataatac ggacttccca
     3661 cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg gttacggttt gtgcgttttg
     3721 ctgcttattc ctccagttta tgcgctttac cgtggcgatt aagcccaaaa actttataaa
     3781 tcgccgtcac cgtcagcata aagatgatgc cgacaaacag cgacatacgc gtatcttcat
     3841 taaagtacat gccgatcaaa acgcaaatca ggaacgccat tgttacgtag ttggcccacg
     3901 ggaacaggat tgagcggaac ggatggctgg caattgctgc tttatgcgca cgacgaaaac
     3961 gcagctggct tatcaatatc acaaaccatg gcaccatccc cggaagcacg ctggcactgt
     4021 agacgtaaac aaacacacgc tgcggattgg gaatgatgta gttcaggcat gagccaatta
     4081 gcagaatagc aatagatact gccacacccg caaccggtac gccgtgacgg gaaactttcg
     4141 ccattgccgc cggtaactga cggtttttcg ccagtgcgta gagcatacgt ccgcaactgt
     4201 acatgccgct gttacagcca gagagcgcag ccgtcagaac cacaaagttg ataatgcccg
     4261 ccgctgcggt aatgccgatt ttggcgaaag tcagtacgaa cgggctaccg ttgctgccta
     4321 tttcattcca cgggaagata gtaacgataa cgaaaatcgc gccaacgtag aaaatcagga
     4381 ttcgccacag caccttgcct acggcactgc gtagcgtcac ctgcggattc ttcgcttcac
     4441 cggcagtaat gccaatcagc tccacgccct ggtaggacgc caccacaata cacaatgcgg
     4501 tcaggaaccc tttccagcca ccagcaaaga aaccgccatg ctctgtgaga ttgctaaaac
     4561 caatcgactg cccgccattg ccaaagccaa agaaaatcac gcccaggcca atgacaatca
     4621 tcacgataat cgtagtgact ttgatcatcg cgaaccagaa ctcgatttcg ccgtacaacc
     4681 tcaccgcagc cagattcgcc aacgccacca gcgccaccgc gatcaatgcg ggtatccact
     4741 gcgccatctc cgggaaccag aactggacat aaacgccaat ggcggtgatt tctgagatcc
     4801 ccaccgccat ccacataaac cagtaagacc aggcggtgag ataaccaaag aacgggctca
     4861 tataacgatg cgcgtaaacg gcgaacgaac cggtaaccgg ttcgaggaac aacatttcgc
     4921 ccattgaacg catgatgaaa aagacgaaca gcccggcgat gatataggcc aacaatacgg
     4981 atggcccggc ccatttcagg gtactggcgg cccccataaa caggccgacg ccaatggtgc
     5041 cccccagggc gatgagttcg atatgtcgag cttccagccc acgctgtagc tctggtttgt
     5101 tatctgccat aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg gttatcgtta
     5161 tgggtacatc gagtgttgcg aatgttttcg taattcagga gaaatggcaa ataaagcatt
     5221 aaaaatttga atgctttgtg taataaaaaa gcagacaggc gacgaagtga ccactccgtc
     5281 gctttacaaa gagaggaaaa tcagaggttg ccggtgtaat gccagcgtaa ataacgcagc
     5341 aaacgaagct gacgcttaat gcggctcggc tgcgaaagca ggcggtagag ccactccagc
     5401 cccagcgttt gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac atcgtaggtc
     5461 ccgccaacgc ccatatacag cgcatctgga tgcaccagac ggcagtcgcg catgaagatt
     5521 tcctgctttg gcgatcccat cgcaacggtg acgatttgcg ctccgctggc atgaatgcat
     5581 tcaaacagcg cctgacgctg ctcgggttta aaataaccat cctgactgcc aacgatattc
     5641 acattccact ggttgcgcag tttagcttca gtttgcgcca gcacttcagg tttaccgccc
     5701 acaagaaata ccggtgtccc ttctttgcct gcgcgcgcca tcagctcttc ccagagatcg
     5761 gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt tacgtactga acgtacaacg
     5821 ctgatgccat ccgcatattt aaattcggca gcgttaatta gctccctgac ctcagcatta
     5881 tcttcaatgg tcagcatttt ttcggcatta atggcaacca gcgttccctg cttaagctgc
     5941 ccgtcagcga acagataatc gagggcgtgc tgcatatcac gccaaccgat caactgtaag
     6001 ccacgcagcg tataggtcgg tgccgtggtg ttgttattca ttgttatcct tcaacctgcg
     6061 tccggagcga tgattttgta cgtttatgaa tgagtccggc gctttcaaaa agccagtaca
     6121 acagttttgc gatcatcaga catgcgccga agaccacgat aaaaaagacc acgcgtgaga
     6181 caaacgaatc cagcccttca cgcgccagca cgatcatatt gaagatcgcc ccgaagcaga
     6241 aactgtgcaa tatcgcagcc ttatagcgat tagtctcgcg gttgcccagc tcatacagcc
     6301 agtcgaacca tttgataatc agtccaacca caatcgcccc gagcgggatg aacaacgcgc
     6361 cgcccatcac caccagtgag cctataagcg taggcgagat cgccagtccg gagtggttat
     6421 tcagcacttc ccaggtaaag tagttggcgg agttcagcac catactcggg cgacccggcc
     6481 acagccagga agggataaag acatagaaat cgcggacaat gggagccagg ccctggaagt
     6541 cgatgttgtc gtagttctgc aacagcaacg ccagattctc ccacggcgag aaggtgtcgc
     6601 gagtgagata gagaaacgta tagaacgctt catcgccgct cacattcatt ccatagcgtt
     6661 ttagcgccag ccagaacatg cccacaatcc ccagcacgcc cgccgccgcc agcatccaca
     6721 acgaaatcca gccgcgaata atgccaataa agaggaagat agcgaatgcg ataatgatat
     6781 tggcgcgagt gccgccgaca atcatgtaag tcaacaagcc aaaggcaacc gtgctgacga
     6841 ggaaaaacag ccacgctttg ctgtcctggc gcagaaagta gaccaccagc atcgccggga
     6901 tgaaaaagta aaagaagcgt tttaacgcca cgccggagac ttcactggag aagatctgac
     6961 tgtaggagtt aagccggaac agcaaaaagc cgttgtgcat gaagaagatg ccgacgctta
     7021 ccagcgcgat gcccatcagg atcacccacg tgagattggt ttccacgcgg ttcatggtaa
     7081 acagcggacg gcgcggtaca tcagcaacgc gtttgcgtag gcgggttttg taggtgacgt
     7141 agtaaaccgc gtagaagcag cccgcagaaa gcaacgcctg caacaagatt tctggcggcg
     7201 cgacaccaac atcaaagcga aaaaccagca cgctggtcag cgggaagccg aagaaaaagg
     7261 tgagcaaaaa cagcaatgaa aagaagacat tgaagttaaa gcgcacacgg cgaaactcaa
     7321 accaggtcag cgtggcaata aacagcgtgc agagcagcca gacaacaaac aggccactga
     7381 attgcagcag actcatgcga cctccccggc ggcaatcgcc aacgcccgct gccagccttg
     7441 tagatagtta gggctaaaga aggcaatggt gtttttatcc accgacgcca actggcgctg
     7501 cgcttcacgc acaatatcct cgttgagatc gtcggtagta aacagcactg gcaaatgctg
     7561 ttccgtcata tcctgccaga acggattttc ccggttaagc acacaaggaa tgcccgcctg
     7621 aatcagtaag cacagcgtac caatgccctg ctggcgggca aaaataaagt aaccaagatc
     7681 gcactgacga agtagcgtca gataggcgtc aaattccagt ttttcgctca gaacttgcag
     7741 attttcttcg ctgaataact ccagccccgc ctgacgaact tcctcaatgt acgcttcgtt
     7801 attgggcgga tatcccatcg gcaccaccac ttttaccgta tcgccaaatt gctgatgaac
     7861 ggcacgtaag gcggcgacat gctcattgct gcggtcgccg gagttcccca ccagaatggt
     7921 cattttccct tcacgttgcc gatcgttcgc catcgtatta agagaagggt ccatccgcgt
     7981 cgggaagtac agcagttctc cccgcacctt tggatgcgtt ttggcaaaaa agctcaaatc
     8041 accgcgggtg gcaaatacac agccgactct cttttgcgcc aggcggcgta gcgggtaaaa
     8101 aagcttatat ctcaagccac tggaaagctc atacaggtct gctccccaga tatgccagaa
     8161 aaactggctg ggcttaatgc caccactcag cagagccagc cacagtttgg gattgaactg
     8221 accgtggaaa aaaaatcgct gctgacggtt agcttttgct ttcgcgatga ccgcttccgc
     8281 cagcgatttt ttcccaggga aaaattgcac agaaagcgcc ggacagctat cacttaagcc
     8341 gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg ctcgtcgcgg ccagcgcgtc
     8401 gttgaaaaac cgcagaacgg ttcggttatg gtgagggata tccgatccca gtacgtgaat
     8461 cagtacagtc atgcccgcct acgccagagt aaaaacacgc cacaacaaag agaaaaatag
     8521 acgatatagg ttgccatata ggcctgtgct gcgcccagtg cgccatgcgc agggattagc
     8581 cagttggcaa ataccatcaa taaagtgaac tggctgattt ccgccagaat ataaaaccgc
     8641 agtgacgctt tggcgatcac cagataacca aagacataag cgcccacttt taacacatca
     8701 cccaccaact gccaggcaaa gagatcgcgc atagcggtaa atttattcga caacagcagc
     8761 cagatagcaa aatcacgcag caaccagacg gtaaaactcg ccgccgccac tgccggtaag
     8821 acaaatttca gcgatttaac cacttcccgg gtgatatcgc gcttttccgt taaccgcgac
     8881 aacgtgggca gcaaatatac gctgaacgat gccgtaataa attgcaggta ggcatcggaa
     8941 atactgctca ccccttgcca gatccccact tcatcccagc tatactgcgc cgccagcagt
     9001 ttacgcatca tgatgtaagc aacaggcaag gtcaccgacg taatcaacgc catgagcgta
     9061 aatttgctca actgccctgc cagaccgtta tcccagctgg gtttcagata gcttaacggg
     9121 atgacaccac gtttgatcaa catgataaca gcaggaatta ccaccagcgc gggaatcagc
     9181 gccagaccca gcaacgcccc ttcataaccg cccaaacgat aactgacgta gtacgcgaga
     9241 acgccaatca agctaccgac aatcagagat aacgcattac ctgcggcatc gcgaaagcct
     9301 ttcatcaacg ccagtaacag gttgccccag gcaatcccca tttgcaccag cgccactaaa
     9361 cgcaccagcc cctgatagtc ggtattacca aacaacccct ggctgattgg cgcagctgcc
     9421 agcacaaaaa ccagcgccat tagcgtagag aagccaagta ccatcgctga tgaagtgccg
     9481 actacgcggc gcagctgttg gggattatca tgatactggg caacgtattt ggttacgccg
     9541 ttaaagatgc ctgccccggc aagcacgccg agcacggtaa tcaactggcg gaaatttgcc
     9601 gccagcccaa gccccgccgg accaaatgac actgccagca acttaccgac cagtaacccg
     9661 gcaccaatct tgaccagtgt actggccgcc gtccacaagg acgcttttgc caacgacata
     9721 tcaggagaag tagttcaaca aggtcgcaat taccgtacgc tgattgacgg gcgacaggtt
     9781 gtagaacaac ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc gatcttcgcc
     9841 gtggaactca ccaaagcgtt cccccgcagg gcaaccgtgc agcggaatgt aatggaacac
     9901 cgccatgatt tccgcttctt tcagaaagtt aatcaatgcg ctccggtcag caatatcccg
     9961 cagtttaatg tagaacatat gcgcgttctg cacgcagcca tcgggaatcg acggcagctc
    10021 gatacgccca ttttttgcca gaggcgccag cgcatcgtag tagttttgcc acagcgccag
    10081 acgttgctgg ttgatacgat ccgcagcttc cagttgcgcc cacaggtacg cagcttgcag
    10141 atcggacatc aaatagctgg agccaatatc gcgccaggta tatttatcga cctgaccacg
    10201 gaagaactgg ctgcggtttg tgcctttttc acggatgatc tcggctcgtt cgatcaacgc
    10261 tttatcgtta atcagcgtcg cgccgccttc accacccgcc gtgtagtttt tggtttcatg
    10321 gaagctaaag cagccaatat gaccaatggt tcccagcgca cgccctttgt aagtggacat
    10381 cacgccctga gcggcatctt ccaccacaaa cagattatgc ttcttcgcca acgccataat
    10441 ggtgtccatt tcgcaggcca cacccgcgta atggaccggc acgataacgc gcgttttgtc
    10501 ggtgatcgcc gcttcaatca gcgtttcgtc gatgttcatg gtgtccgggc gaacatccac
    10561 aaagacgatt ttcgcgccac gcagcacaaa ggcattggcg gtggagacaa aggtgtagct
    10621 cggcatgatc acttcatcgc caggctggat atcgagcagc agcgccgcca tctccagcga
    10681 agcggtacag gatggcgtca gtaacacttt ggcgctgcca aaacgttgct ccagccactg
    10741 ctggcagcga cgggtaaaac caccatcgcc gcacagtttg ccgctaccca ttgccgactg
    10801 catatagtcg agttcggttc ccaccaccgg cggtgcgtta aatggaatca tgtgatcacc
    10861 tgtataacca gtacgcggtg ctttctacat tcgcaccact ttgtatgtat cgtttaagcg
    10921 cggcggtgtt gcccatctgg gtcgccaccc gcaaagttgt tttaccgcga gcatacgccc
    10981 agtttagcgc tgtttgcatc agctcagcac ctgcaccgcg tccagccagc aggccaattc
    11041 gcgcatctgt cgcattgagt tcccgtaaag agacatagcc gcgaatatcg ccggacgccg
    11101 cacgtaaaat cagacattga tgatcaaagg tgccgcgcac ggcattttca atccactgcg
    11161 cataaaagcg accgctggcg tcaggcgcat accatggcgc acgaaaacgg ctttgcgcaa
    11221 atgcggcgct ggctaactga cgtaatgcgg gaatatcggt ctcttgtgcc actacagcac
    11281 cgctatcact gacattgttc acgggtagcg ccaaatcaac ttcaccttct accagggaga
    11341 atcccagctg ttgcagggca tccagttcac ccgtatttga tgccgcaatt ttggcctgca
    11401 cccgtgacca cggcgctaac gcgtctggcg tcaggagcgg tgcttcagac gtaatgcgca
    11461 caatggcact gttaacacca aagaaggcgt tttcccaggt tagtggctca atactggcgc
    11521 ggacgggcac gaagtaactc cagcagatat tggccgtagc cagttttcgc cagcgagctg
    11581 gcggcgcgct tcacgccttc gtcatcaagc cagccgttac gccaggcgat ctcttccagg
    11641 caggcaatct taaagccctg gcgtttttcc accgtctgta caaaggtgct ggcttcaatc
    11701 aggctgtcgt gagtgccagt gtccagccag gcaaatccgc gcccgagcag ttcaacggtc
    11761 aggttgcccg cctcgaggta catctggttg atggaggtaa tctccagttc accacgctcc
    11821 gacggcttca cctgctttgc gtactccaca actttactgt cgtagaaata aagcccggtc
    11881 accgcccagt ttgattttga ctgttttggc ttttcttcca gagagatagc gcggaaattg
    11941 tcgtcaaact ccacgacgcc aaagcgttct ggatccatta cctgatagcc aaataccgtc
    12001 gccccttgcg tgcgcgccgc aacatgacgc agcttcggac tgaagccctg accgaagaag
    12061 atgttatcgc ccagcaccag acaagatggc tcaccattaa ggaaggtttc accgatgata
    12121 aatgcctgcg caagaccgtc cgggctgggc tgttcggcat attccagctg gataccgaac
    12181 tcactaccat cgcccagcag gcgctggaaa taacctttgt cttccggcgt agtgatgatg
    12241 agaatttcgc ggataccggc cagcatcagc accgacagcg gatagtaaat cattggctta
    12301 tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa tcgggtgccg
    12361 gaaccgcccg ccaggataat acctttcatg tttgcctccg ctgagaatta gccgttcaga
    12421 cctaaacgct cgccctgata gctgccgtcc tgcacctgct tccaccagct ttcattagcc
    12481 agataccact gaaccgtttt acgcattcca ctttcaaagg tttcctgcgg cagccagcca
    12541 agttcacggg caattttcga agcatcaatg gcatagcgta ggtcatgccc tggacggtca
    12601 gcgacaaagg tgatcagatc gcgatagtgc gccacgccgt gcggcttgtt cggagccagt
    12661 tcttccagca gctcgcagat ggtttccaca acctcgagat tcttacgctc gttgtgaccg
    12721 ccaatattat aggtttcacc gactttcccg gtggtcgcca cgcaatacag cgcgcgggca
    12781 tgatcttcaa catacagcca gtcacggatt tgctgcccgt tgccatatac cggcagcggt
    12841 ttacccgcca gcgcgttgag gatcatcagc gggatcagtt tttccggaaa gtggtaagga
    12901 ccgtagttat tcgagcagtt ggtgataagc gtcggcaaac cataggtccg cagccaggcg
    12961 cgcaccagat ggtcgctgct ggcttttgac gcggaataag ggctgctcgg cgcatacggc
    13021 gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac catatacttc gtcggtggag
    13081 atatgatgaa aacggaacgc tgatttttta tcttccgtca gcgcagtcca gtaagcccgc
    13141 gccgcttcaa gcagtgtata agtcccgaca atgttggttt caataaacgc tgccgggccg
    13201 tcaatagaac ggtcaacatg gctttcggct gccagatgca tgacacagtc tggctgatac
    13261 tcagtgaata cgcgcgccag ttctgcccga tcgcagatat caactttctc aaaggcaaag
    13321 cgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc cggcgtaggt cagcttatcg
    13381 accaccacca ccgcgtcgct cgtttcgttg atgatataac gcaccagcgc cgagccaata
    13441 aacccggcac cacctgttat cagaatcttt ctcatcagcg ccagactcct ttggcatcga
    13501 cgacatactg ctgatggacg ttgtcgccat tgataacttt gaactgacta tgatcgacca
    13561 gcatcaccag cacatctgcc gttgccagcg cctcgtcaag ctgcgccaga gtacaaagcc
    13621 cggtcagttt cttcggcaac tggtggatgt taggctcaac aaccagcgtt tcgccgctgt
    13681 gccactgggc gatcagttca gcgatttcca tcgcagggct ttcacgcagg tcatcaatat
    13741 tcggtttaaa cgccagacca aagcaggcga ttttcagttc actggcgcgt ttatcggtag
    13801 cagcaaggca gtcggcaacc gccgctttca cctgatcgat aacccagaac ggtttgtgat
    13861 cgttcacttc gcgcgcggta cggataagcc gcgcctgctg cgggttctgt gccacgataa
    13921 accacggatc aacagcaatg cagtgaccgc ccacgccagg accaggctga agaatattaa
    13981 cgcgagggtg acgattcgcc aggcgaatca gttcccagac gttaattccc tgatcggcac
    14041 aaatcagcga cagttcgtta gcaaaagcga tattcacatc gcggaagctg ttttcggtga
    14101 gcttacacat ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg aggaaaattt
    14161 tgtacagttc gctggcgcgg gccgaacaaa ccggcgtcat accaccaatc acgcgatcgt
    14221 ttttaatcag ctcgaccata acctggcccg gtaacacgcg ttccgggcag taagcaatgt
    14281 tgacgtccgc caactcgccc acctgctgtg ggaaagtgag atctggacgc atctctgcta
    14341 accattctgc catcttctcg gttgacccca ccggcgaggt ggattcaagg atcaccagcg
    14401 cgcctttttt cagcactggc gcaatggagc gagcagccga ttcaacgtag gtcatatctg
    14461 gctcatgatc gcctttaaac ggcgtgggta cagcaatcag ccaggcatcc gcttcaaccg
    14521 gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg ctcgccaaat
    14581 caggctcgac gatatggatt tcgccacgat tgatggtatc aactgcgtgt tggttgatat
    14641 cgacaccaat cacctgtttt tgccgagagg caaacgcagc tgctgtaggc agcccgatgt
    14701 aacccagtcc gataacagaa atggtcgcaa aactcatagt gatatccgat tattttttaa
    14761 cgcttccaga atgcgagagc atgcctgacc atcaccatac gggttatggg cgcggctcat
    14821 agcttgatat tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa ttcgctgctt
    14881 atccgtgcct accagacgca ccgtacccgc cgtcaccgct tccggacgct cagtggtatc
    14941 gcgcattacc agcaccggtt tccccagcga aggcgcttct tcctgaatgc cgcctgagtc
    15001 ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac ggtaaatact cctggggatc
    15061 gatcagaatg acatttttca cgtgccccag aatgcgattg accggctctc tgacgttcgg
    15121 gttgagatgc accggataga caatctggat gtcctggtgc gtggtggcga tgtctgccag
    15181 tgcatggcag atttcttcaa aaccacgacc aaagctctca cgcctgtgac cggtcaccag
    15241 aatcattttt ttatcagggt cgataaacgg ataatttgcc gccagttctg aacgcagcgt
    15301 atcgctgctc atcacctggt cacgcaccca taacagtgca tcaataacgg tattaccggt
    15361 aatgaagatt cggctgtcag caacgttttc acgcagcaag ttttgccggg aagtttcggt
    15421 tggagagaag tgatacatcg ccagatgccc ggtcaatgta cggttagcct cttccggcca
    15481 cggcgaatag agatcgcccg tgcgcagacc agcctcaacg tgaccaacag gaatacgctg
    15541 ataaaacgcc gccaggctgg ttgccagcgt tgtagtagta tcaccgtgaa ccagcacgac
    15601 gtctggtttg aactcggcaa gaattggttt tagcccttcc agaatccgac aggttatctc
    15661 tgtaaggccc tgtcctggct gcattatgtt gagatcgtag tcaggtacaa tggaaaagag
    15721 tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt tagcctcaaa
    15781 aaaaggatct tttgccaacg catgcaccaa cggtgccatc ttgatggctt ccgggcgcgt
    15841 accaaataca gtcagtactt tcacatcgat tctcttcgaa taagcggcga gcgcctttgc
    15901 gctcaccgca gcagtgttgc tatttcgagc aacggcgggt taatgcgaca ccagccccga
    15961 tcagcccccc gacaatgccc cacataatca tcaggaaggc acgacgtggg ctatcgcgtt
    16021 ttaccggttc ttccggcgta cgcaaatagc gataggtctg aaaacgcgga tccagggttg
    16081 gaccaacatt cagggtgttt aacatggccc gattttgatc atagtcgaga tcaaaggccg
    16141 gaccgacggc ctgtaaattt tccagtcgag cctggagcat tggacgccca agcaggaaca
    16201 tttctgaatc aggtaattcc tcggcaggca catctgtcgc actgcgcgaa atattatgct
    16261 gctcagcaat tttcagcgcc tgttcaatac tgttcatccg gcgatcgtag atagctttcg
    16321 ccacctcttc ctgacgcttc acctgagatt tcatctggat ggtgcgtgcc gcccatgcgc
    16381 ctttcagctc atcattcaga tggctggctg cacgctggct ggcaaaagca acatactgac
    16441 gtaacaggtt attagcgtca ggcgcagttt cggcaatcag cttcacgctg tcattgaccg
    16501 cgcgggtgaa gtcaccggga ataaacacga tgttgttgat catttcatcc agcaacgccg
    16561 catcggcttt gctattgccc accatccgct gtttgtaata gtcggtttgc agccagaact
    16621 ctctgcgggt atcccacgag gccagttgca taacaaactc tttataggct tcgtccatga
    16681 ccgatggttg gtcggcagaa gccatgtttg aacggacatc caggttacgc aaaaattgct
    16741 gctgtgagta atatcccccc agcatattca ccgttggacg atcggtaatc gctgtcgagc
    16801 tccactcctg gcgagcaaaa aaagtatacg ccagcgcgat taacgcaaac gccagcccca
    16861 tgccaataat ccacagcttc ccagcccaca aggtacgaaa caacccacga atatccagtt
    16921 cattttcagc gtcttcggcc ggtttcccag gcattggttg tgtcatcaca tcctcattta
    16981 tttggttaaa ttggggctgc caccacgatt tctacgcagt ctgcgtttta cgcgcttaat
    17041 aaagcgagca actttccagg cacgcttgat gcaatatcca tagaggaaga atgctagcaa
    17101 aaagagcacc agcatgaccc actccgggac aaaatgagaa tattctgcca gcacgccaat
    17161 ggaagcgagc agtgctgcgg caagggtaat cagcacaaac gcctggcggg aagtaaaccc
    17221 ggcacgcatg atcaaatggt gaatatgctg acggtctgga gagaatgggc tcatgccttt
    17281 acgcaggcga cggtacataa tcgccaccat atccattagc ggaatggcga ttatccacaa
    17341 agcggtaacc gggctgatgg gatgagtttt gccctgggtc gtttcgagca ggatccagat
    17401 aacggtaaaa ccaatcagcg tactgcccgc atcccccata aagactttgt agcggcgacc
    17461 caggatacca aggttaagca tgatgtatgg caggatggcg gcgatcatcg caaagcacca
    17521 gattgcgaga ctggtttgcc cgtcgaacca caaaatcata ccgattgctg caaacgagac
    17581 gcaggacaac ccgcccagca agccatcaat gccatcaacc atgttgaacg cattaatggc
    17641 cgcccagacg gcaaatagcg tcaggaagta accaaacggt ccgagcacca tctcccagga
    17701 gccaaagata taacccaggc tactgagata aagtttgccg aacaccatca taacaatgcc
    17761 aacagcggcc tgtatggtgg cacggatttt tacgctgata tcaaaacggt catccagcgc
    17821 gccaatgaaa acaagcacac cagcacaagc gagatagaga gatgcatgcg gaatatagta
    17881 atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc caacgagagg
    17941 tatcaatccc tggtgacgtt tgcggaagtt tggtttatcc actaaaccga ctttttttgc
    18001 caccttacgg gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga tgagatcagt
    18061 actcactgtc agtaaattca caatgcatgc tctcagagaa aattattagc agaagtataa
    18121 ccacgaagac ctttattcag aaaggaaagt ccgttccgaa cctaccagca ctatgcgaaa
    18181 attaactact tgatatgcaa attgcataac acaccagcaa attccaggaa ttttcctaac
    18241 atcgccagcg cggcattaag attacagata taaaagcaaa acgccacgta aacacgtggc
    18301 gtttttggca taagacaaat ttatgagcgt ttcatcattt cgaagaaatc gtcattggtc
    18361 ttggtcattg ccagtttatt aatgaggaat tccattgcat cgatttcgcc catcgggtga
    18421 atgattttgc gcaggatcca cattttctgc agttcttcct gagtcgtgag cagctcttct
    18481 ttacgggtac cggaacggtt gtagtcgata gccgggaaga cgcgtttttc agcgatctta
    18541 cgagagaggt gcagttccat gttgcctgta cctttaaact cttcgtagat aacttcgtcc
    18601 attttagaac cggtatcgat aagcgccgtc gcgataatgg tcaggctgcc gccctcttcc
    18661 acgttacgcg ccgcaccgaa gaagcgtttc ggacgatgca gggcgttggc gtccacacca
    18721 ccggtcaaca ctttacctga cgccggaaca acggtgttgt aagcacgcgc cagacgagtg
    18781 atggagtcga gcagaatgat aacgtctttc ttgtgctcaa ccaggcgttt cgccttctcg
    18841 atcaccattt ccgcaacctg aacgtggcga gatgcgggtt cgtcaaaggt agaagcaaca
    18901 acttcacctt ttaccagtcg ctgcatctcg gttacttctt ccggacgttc gtcgatcagc
    18961 agaaccatca gcacacaatc cgggtggttg taagcaatgc tctgagcaat gttctgcagc
    19021 agcatggttt taccggcttt cggcggtgcc acaatcagac cacgctgacc acgaccgata
    19081 ggtgatgcca gatccagtac gcgagcggtt aaatcttccg tagaaccgtt accacgttcc
    19141 atacgcagac gagagtttgc gtgcagcggg gttaagttct caaagaggat tttgttgcgg
    19201 gcgttttcag gtttgtcgaa gttaacttcg ttaactttca gcagcgcaaa atagcgttca
    19261 ccttctttcg gcgggcgaat cttaccagag atggtatcac cagtgcggag gttgaaacgg
    19321 cggatttggc taggggaaac gtagatgtca tcaggaccgg cgaggtagga gctgtctgcg
    19381 gaacggagga aaccaaatcc atcctgcaat atctccagta cgccatcacc aaagatatct
    19441 tcgccactct ttgcgtgctg cttcaggatg gcaaaaataa tgtcctgctt acgcatacga
    19501 gccaggtttt ccagccccat attttcgccg agagtgatca gctcagaaac cggcgtattc
    19561 tttaattcgg taagattcat agtggtgtga gttcttaaac ttggggtaaa tctcgaactt
    19621 aatgttgtga atggtatggc aggatcatcc atgcctgttt acggtcatcg actcatgtct
    19681 tttcgctgcc tggtcacagg agagtacgca gaactgaaac gacaagacgg attgagtgac
    19741 gagccagaaa tctgttcact tcgcatttaa gataaaacgg gaagcgttgg aaattacaag
    19801 attcaaactt aataaggtat gtttaatacg aagtcaacac taagttagca tgactcacgc
    19861 cgggcgtcca gtttttagcg acggggcacc cgaacatgaa attcccttac gccaggttag
    19921 cgtcgaggaa ctctttcaac tgacctttag acagtgcgcc cactttggtt gccgccactt
    19981 caccgttttt gaacagcagc agagtcggga taccacggat gccatatttc ggcgcagtgc
    20041 cagggttttg atcgatgttc agttttgcaa cggtcagttt gccctgatat tcgtcagcga
    20101 tttcatccag aatcggggcg atcattttgc acggaccgca ccactctgcc cagaaatcga
    20161 cgaggatcgc cccgtccgct ttgagtacat ccgtgtcaaa actgtcgtca gtcaggtgaa
    20221 taattttatc gctcatatat aactccacag gaataagcct ggcgtgttgg tttcgttgtt
    20281 ggtgtaacat taaccaacta aaggttgact ttatttcacc ggatacgctt tcgtaaagca
    20341 atagtaagct gatattctac cacactatga gcaaaacaca tttaacagaa cagaagtttt
    20401 ccgacttcgc cctgcatccg aaggttgtag aagcccttga aaaaaaaggg tttcataact
    20461 gtacgcccat tcaggcactg gcccttccgc tgacgctggc gggtcgtgac gtagccgggc
    20521 aggcgcaaac cggtaccggg aaaacgatgg cgtttcttac gtcaacgttt cattatcttc
    20581 tctctcatcc cgcgattgcc gatcgcaagg tgaatcaacc gcgtgcctta attatggcac
    20641 cgacgcgtga acttgccgtg cagatccatg ccgacgcgga accgctggcg gaagctactg
    20701 gcctgaagct gggtctggct tacggtggtg atggctacga caaacagctg aaagtgctgg
    20761 aaagcggcgt tgacattctg attggcacca cgggtcgttt aattgactac gccaagcaga
    20821 accacattaa cctcggtgcc attcaggtgg tggtactgga cgaagccgat cgcatgtacg
    20881 atctgggctt tattaaagat atccgctggc tgttccgccg tatgccgcct gcaaaccagc
    20941 gcctcaacat gctgttctcc gccacgcttt cgtaccgggt acgtgaactg gcgttcgagc
    21001 agatgaacaa tgccgaatat attgaagtgg aaccggaaca gaaaacgggc catcgtatta
    21061 aagaagagct tttctaccct tctaacgaag aaaaaatgcg tttgctgcaa acgctgatcg
    21121 aagaagagtg gccagacaga gcgattattt tcgccaacac caaacaccgt tgtgaagaga
    21181 tctggggcca cctggcggca gatggtcatc gtgtcggttt attgacaggc gatgtcgcgc
    21241 agaaaaaacg tctgcgtatt cttgatgaat ttacccgtgg ggatctggat attctggttg
    21301 ccaccgacgt tgccgcgcgt ggtttgcata ttcctgctgt gacacacgtc tttaactacg
    21361 atttacccga tgactgtgaa gattacgttc accgtattgg tcgaacaggt cgcgcaggcg
    21421 caagcggtca ctctattagc ctggcgtgtg aagagtatgc attgaatttg cctgctattg
    21481 agacctatat tggtcactca attccggtaa gtaaatacaa tccggacgca ttgatgaccg
    21541 atctgccaaa accgctgcgc ctcacgcgcc cgcgcacagg caatggtccg cgtcgtactg
    21601 gcgctccgcg taatcgtcgt cgttcaggtt aaagaaaaaa tgtcatactc caaatcactc
    21661 aagctgtatc aagatggcaa gagaatgaat cccgatgcgc ttactgtcgt aagtgattca
    21721 ggtgaaggaa tgcagccaac gcagagacag attgaaggat gaagagtatg ggttccacct
    21781 cgtcgctgta tgcagccatt gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg
    21841 aggtggctgg aagcatccag acgctgacgc gaataaaacg caaagtgcgt ctggctgccg
    21901 gcctgaatag cgaaaatgcc ctgtctaatg aagcaatgga gcgcggttgg caatgtctgc
    21961 gcctgtttgc tgaacgtctg caagatatcc ctccctcgca aattcgcgtt gtcgctacgg
    22021 cgacattacg acttgccgtc aatgcgggtg attttattgc caaagcgcag gaaatcctcg
    22081 gttgtccggt acaggtgatc agcggtgaag aggaagcgcg tctgatttat cagggcgttg
    22141 ctcacaccac tggcggtgcc gatcagcgcc tggtggtgga tatcggcgga gccagtactg
    22201 aactggtaac cggcacgggt gcacaaacca cctctttgtt cagcctgtcg atgggctgcg
    22261 ttacctggct ggaacgctat tttgccgatc gtaatctggg gcaggaaaat tttgatgctg
    22321 cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg tatcacggct
    22381 ggaaagtgtg cgttggcgct tccggcaccg tgcaggcgtt acaggaaatc atgatggcac
    22441 aggggatgga tgaacgcatt accctggaaa agttgcagca actgaaacaa cgcgccattc
    22501 attgcggtcg gctggaagag ctagagattg acgggctgac gctggaacgt gcgttagtgt
    22561 tcccgagtgg tctggcgatc ctgatagcca tttttaccga actgaatatt cagtgtatga
    22621 ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg tatgttgcat cttaccgtcg
    22681 agcaggatat tcgcagccgt acgctgcgta atattcagcg ccgctttatg atcgatattg
    22741 atcaggcaca gcgcgtagcc aaggttgcgg ctaacttctt cgatcaggtg gaaaatgaat
    22801 ggcatcttga agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg
    22861 gcctgagcgt tgacttcaaa caagcgccgc aacacgctgc ttatctggtg cgtaacctag
    22921 atcttcccgg ttttaccccc gcacagaaaa aactgctggc gacgctactg ctcaaccaga
    22981 ctaatccggt cgatctctca tcgctgcatc agcaaaatgc cgtaccaccg cgcgtcgcag
    23041 aacaactctg ccgtttactc cgcctggcta tcatttttgc cagccgtcgc cgtgacgatc
    23101 tcgtgccaga gatgacatta caggctaacc atgaactgtt gaccttgacg cttccgcaag
    23161 gttggctaac ccaacatccg ctgggtaaag agattattga tcaggaaagc cagtggcaga
    23221 gctatgtcca ctggccgctg gaagtgcatt aatgagtaag tgccggatgc gatgctgaca
    23281 catcttttcc ggccttgatt atttccctcg ttttgccgcc atcatcgctt tcagattcgc
    23341 cagatggctt tgccctttct gcatccgttc ttcggcgctg accactttgc gctcctgttc
    23401 ccaaatcaaa tcatcctgcg gcagctccag caggaagcgg ctcggctccg ggcgcaccag
    23461 ttcgccgtac tgacggcgtt ctttacacag cgtaaaggtc aattccttct gggcgcgggt
    23521 aatgccgaca taggccagcc gccgctcctc atcaatattg tcttcatcga tgctgctctg
    23581 gtggggcaaa aacccttctt ccataccaac catgtagaca tagggaaact ccagcccttt
    23641 cgacgcgtgc agagtcatca gttgcacctg atccagctct tcctcgctct cgccgcgctc
    23701 catcatgtcg cgcaaagtaa agcgcgtcac cacctgggtg agcgtcatcg gctcatccag
    23761 ttcgctgcct tcgagcatct ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg
    23821 catttcggcg gctttcgggc tgggcgatgt ttcgtacagc caggattcat aatccatgcc
    23881 gtggatcaga tcacgcaccg cggcaatcgg ctcccgctcc gccagacgct ggatttccgc
    23941 caaccagtga gtgaagcggg tcaatgcttc ataaccacgt ccgctcaggg tctgactcag
    24001 gcccatatca aagctggcgg taaacatgct tttattgcgc gtcatcgccc attcacctag
    24061 ctttttcagc gtcgccgggc caatctctcg cttcggcgtg ttaacgatac gcagaaatgc
    24121 gctgtcatcg tccgggttag tcagcacgcg cagataagcc agcaagtcct tgatttcagg
    24181 acgagagaaa aacgacgtac cgccagatat tttgtacggg atgcggtttt gcatcaggaa
    24241 tttttcaaat acccgcgact gatggttgcc gcgataaaga atggcgtaat ctttgtactg
    24301 cgttttattg acgaagtgat gggcgatcag ctcgccggtg acgcgctcgg cttcatgttc
    24361 ctcgttattc gcgcttaata ctttaagttc agcgccgtaa cccagctcag aaaagagtcg
    24421 cttttcaaag acatgcgggt tattagcgat aagaatattc gctgctttca ggatacgccc
    24481 ggaagaacgg tagttttgct ccagcttaac cacctgcagc gccggaaaat ctttgctcaa
    24541 caggaccagg ttctgcggac gtgcgccgcg ccatgaatag attgactgat cgtcatcacc
    24601 caccacggta aaacgcgcac gctttcctac caacagctta accagttcgt actggctggt
    24661 attggtgtcc tgatactcat ccaccagcag ataacgaatt ttattctgcc agcgctcacg
    24721 cacctcttca ttacgttgaa gcagtagcgt cggtagcaga atcagatcat cgaaatccag
    24781 cacattgcac gccttcatat gcgcgtcgta caggccgtaa cagtgggcaa aaatacggtc
    24841 gcgctcgcct ttggcgctgg ctgccgcctg caccggggtc ataagatcgt ttttccagtt
    24901 ggagatcgtc gagatcaatt gctgaagtat gagcttatca tcttcgataa gcccgtcagt
    24961 caactccttc agcagcgcca cctgatcggt atcatcaaac agtgagaagt tcgacttcat
    25021 ccccaacgcc gcatattcgc gcttgataat atccagccca agcgtgtgga aggtggagat
    25081 catcaaccct cgcgcctctt tgcgccccag cgtctgcgcc acacgctctt tcatctcgcg
    25141 cgccgctttg ttggtaaagg tgactgccgc aatatgccgc gcctgatagc cgcaaccacg
    25201 aatcaggtga gcgattttat tggtgatcac tcgcgtctta ccggaacccg cccccgccag
    25261 caccaggcaa ggtccggtga cgaattcgac ggcttgttgt tgtccggggt ttaaacgcat
    25321 acagatcact caatgaaagt agggagggga aatttagagc atggtagtat agccagcctc
    25381 aaccacccca ctcaaggcac gatcatggca aaaacagcag cagcactgca tatccttgtt
    25441 aaagaagaga aactggctct ggatcttctg gagcagatta agaacggtgc cgacttcagt
    25501 aaactggcga aaaaacactc catctgcccg tcaggcaaac gcggcggtga tttaggtgaa
    25561 ttccgccagg gccagatggt tccggcgttc gataaagtgg tgttctcctg cccggtgctg
    25621 gagccaacgg gtccactgca cacgcaattc ggttaccaca tcattaaagt gctgtaccgt
    25681 aactaacaga aaggccttct ccgggagaag gccttgaact ctgaatcgct attaacccgc
    25741 aacagcaata cgtttcatat ccgtcatata gccgcgcagt ttcttaccta cctgctcaat
    25801 cgcatggctg cgaatcgctt catttacatc acgcagctgc gcgttatcta ccgcaccttc
    25861 cggaatagct ttacccaggt cgcccggttg cagctctgcc ataaacggtt tcagcagtgg
    25921 cacacaagcg taagagaaca gatagttacc gtactcggca gtatcggaga taaccacgtt
    25981 catttcgtac agacgcttac gggcgatggt gttggcaatc agcggcagtt cgtgcagtga
    26041 ttcatagtaa gcagattctt cgatgatgcc ggaatcaacc atggtttcaa acgccaactc
    26101 aacgcctgct tttaccatcg cgatcatcag tacgccttta tcgaagtact cctgttcacc
    26161 gattttgcct tcatactgcg gcgcggtttc gaatgcggtt ttgccagtct cttcacgcca
    26221 ggtcagcagt ttcttatcgt cgttggccca gtcagccatc atgccggagg agaattcgcc
    26281 ggagatgatg tcgtccatat gtttctggaa cagcggcgcc atgatctctt tcagttgctc
    26341 agaaagcgcg taagcacgca gtttcgccgg gttagaaaga cggtccatca tcagggtgat
    26401 gccgccctgt ttcagcgctt cggtgatggt ttcccaaccg aactgaatca gtttttctgc
    26461 gtatgccgga tcggtgcctt cttccaccag cttgtcgaag cacagcagag aaccagcttg
    26521 caacataccg cacaggatgg tttgctcgcc catcaggtca gatttcactt ccgcaacgaa
    26581 agaggattcc agaacgcccg cacggtgacc gccggttgca gccgcccatg ctttagcgat
    26641 cgccatgcct tcgcctttcg gatcgttttc cgggtgaacg gcaatcagcg tcggtacgcc
    26701 gaatccacgt ttgtactctt cacgtacttc ggtgccaggg catttcggcg caaccattac
    26761 gacggtgatg tctttacgga tctgctcacc cacttctacg atattgaaac catgagagta
    26821 gcccagcgcc gcgccgtctt tcatcagcgg ctgaacggaa cgcactacgt cagagtgctg
    26881 cttgtctggc gtcaggttaa ccaccagatc cgcctgcggg atcagttctt cgtaagtacc
    26941 cactttaaaa ccgttttcgg tcgctttacg ccaggatgcg cgcttttcgg caatcgcttc
    27001 tttacgcaga gcgtaggaaa tatccagacc agaatcacgc atgttcaggc cctggttcag
    27061 accctgtgcg ccacagccga cgatgactac ttttttaccc tgaaggtagc tcgcgccatc
    27121 ggcgaattca tcgcgcccca taaagcgaca tttgcccagc tgtgccaact gctggcgcag
    27181 attcagtgta ttgaagtagt tagccatggt gattcctcgt gatgttgtgc ttcttattgt
    27241 tcggttcgcg tttgcgagat tgaattcact atatgacagg aaatttattg cggaaattga
    27301 tatattcaca acgtcacatt gcaatttctg caacgtcaac attaagggct gcgccattgg
    27361 atttacgcga tctgaaaacc ttcctgcatc tggcggaaag ccgccatttt ggccgcagcg
    27421 cgcgggcaat gcacgttagc ccgtctacgc tttcgcgaca aatacagcgc ctggaagacg
    27481 atctcggtca gccgctgttt gtgcgtgata accgcacggt aacgctgacg gaagcgggcg
    27541 aagaactgcg cgttttcgcc cagcaaacgc tgttgcagta tcagcaactg cgccacacca
    27601 tcgatcagca agggccgtcg ctctctggcg aattacatat cttctgctcg gtgaccgctg
    27661 cctacagcca tctgccgccg attctcgatc gcttccgcgc ggaacacccg tcggtggaga
    27721 ttaaactcac gactggtgat gcggcagatg cgatggaaaa ggtcgtcact ggcgaagcgg
    27781 atctggcgat tgccgggaaa ccggagactc tgcccggcgc agtggcattt tcgatgctgg
    27841 agaatctggc agtagtgctg attgcccctg cgctaccctg cccggtgcgt aaccaagttt
    27901 cggcagataa gccggactgg tctacagtgc cgtttatcat ggccgatcag ggaccggtgc
    27961 gccggcgcat tgaactgtgg tttcgacgca acaaaatcag caacccgatg atttacgcta
    28021 cggtcggcgg gcatgaagcg atggtgtcga tggtagcgct cggctgtggc gtggccttgt
    28081 tgccggaagt ggtgctggaa aacagcccgg aaccggtgcg taaccgcgtg atgattttag
    28141 agcgcagtga tgagaaaacg ccgtttgaac tgggcgtgtg tgtgcagaaa aagcggctac
    28201 atgagccgct gattgaggca ttctggaaga ttttgccgaa ccattaaccc gccaaaaaga
    28261 acctgaacgc cgggttattg gtttcgtcgt ggcaatcgta acccaactca ttcaaccggg
    28321 tttcgaaatc cggttcatgg tcgccaagtt cgaacgccgc cagtacgcgc ccgtagtcgg
    28381 tgccatggct gcgatagtgg aacaaagaaa tgttccagtg cgtacccagc gtgttgagga
    28441 agcgcaacag cgcgcccggc gattccggga attcgaagct gtagaggcgt tcctgcaacg
    28501 gatgcgatgg acgcccgccg accatatagc gcacatgcag cttcgccatt tcgtcgtcgg
    28561 agagatccac tacgctgtag ccgccatcgt tgagcatctg caaaatttct ttgcgctctt
    28621 cgagaccacg gctcaggcgc acaccaacaa aaatgcaggc gtttttggca tcggcaaaac
    28681 ggtagttgaa ctcggtgacc gagcgcccgc caagcagctg gcagaacttc aggaagctgc
    28741 ccttctcttc cgggatggtt accgccagca acgcttcacg ctgttcgccc agttcgcaac
    28801 gttccgagac gtagcgcagg ccgtgaaagt tcacgttagc accagaaaga atatgcgcca
    28861 gccgctcacc gcgaatgttg tgctgggcga tatatttttt cattcccgcc agtgccagcg
    28921 cgccagaggg ttccgccacc gcgcgcacat cttcgaacag gtctttcatc gccgcacata
    28981 tggcatcgct atcgacggtg atgatgtcgt cgagatactc ctggcataaa cggaaggttt
    29041 cgtcgccgat gcgtttcacc gcaacacctt cggcaaacag ccccacgcgt ggcagatcaa
    29101 ccggatgacc tgcatccagc gccgctttca ggcaggcgga gtcttccgct tccacggcaa
    29161 tcactttgat ttgcggcatc agttgtttga tcagtaccgc cacgcccgcc gccagaccgc
    29221 cgccgccgac tggcacaaat acgcggtcga gatgggcgtc ctgctggagc agttccagcg
    29281 ccagcgtgcc ttgcccggcg atcaccatcg gatggtcgaa cggcggtacc caggtgaaac
    29341 cctgctgctg tgacagttcg atcgctttcg ctttcgcttc atcgaaattt gcgccgtgaa
    29401 gcagcacttc gccgccaaag ccgcgcaccg cgtcgacttt gatatcggcg gtggcggttg
    29461 gcatgacgat cagcgccttc acgcctaacc gtgcggaaga aaacgcgacg ccctgcgcgt
    29521 ggttacccgc agaagcggta atcacgccgt gcgctttctg ttcttccgtc aggcccgcca
    29581 tcatggcgta tgcgccacga agcttaaagc tgtgcactgg ctggcgatct tcgcgcttca
    29641 ccagaatcac gttatcaaga cgcgacgaga gtttttccat tttttgtagc ggcgtgacct
    29701 gcgccgcttc gtagaccggc gcgcgcagca ccgctcttaa atattcggcg ccttccgggg
    29761 cgccggagag aggttgtgag tctgccatca ttaacccccc agtttcgatt tatcacgcac
    29821 cgcgcctttg tcggcgctgg ttgccaggct ggcgtaggca cgcagcgcaa aggaaacctg
    29881 acgttcacga tttttcggtg tccaggcttt gttgccacga gcttcctgcg cttcacgacg
    29941 cgccgccagt tcggcatcgc ttacctgtaa ctgaatgcca cggttcggaa tgtcgatagc
    30001 gatcaggtca ccatcttcaa tcaggccaat gctgccgcca cttgccgctt ccggtgagac
    30061 gtggccgatg gaaagaccag aggtgccgcc agagaaacga ccgtcggtga tcagcgcaca
    30121 ggctttaccg agacccattg atttcaggaa actggttggg tagagcattt cctgcatccc
    30181 cggaccgcct ttcgggcctt catagcgaat tactaccaca tctccggcga caactttacc
    30241 gccgagaatc gcttctaccg cgtcgtcctg gctttcgtag actttcgccg ggccagtgaa
    30301 tttgaggatg ctgtcatcga cgcctgcggt tttaacgatg cagccgtttt ctgcgaaatt
    30361 accgtatagc accgccaggc cgccgtcttt gctgtaggcg tgttccagcg agcggataca
    30421 gccattggag cgatcgtcgt ccagcgtatc ccagcggcaa tcctgcgaga atgcctgtgt
    30481 agtacgaatg cccgccgggc cagcgcggaa catatttttt accgcatcat cctgggtcac
    30541 gatgatgtcg tattgttcca gcgtttgcgg caacgtcagg ccaagtacat ttttcacatc
    30601 acggttcagt aaccccgcac gatccagctc gccgagaata ccgataacac caccagcacg
    30661 gtgaacatct tccatatggt atttttgggt gctcggcgca actttacaca gctgcggtac
    30721 tttgcgggag agcttatcga tatcactcat ggtgaagtcg atttccgctt cctgcgccgc
    30781 cgccagtaag tgaagaacgg tgttggtaga gccgcccatc gcgatatcca gcgtcatggc
    30841 gttttcaaac gccgccttac tggcgatgtt acgcggcagt gcgctttcat catcctgttc
    30901 gtagtaacgt ttggtcaatt caacaatgcg tttaccagca ttgaggaaca gttgcttgcg
    30961 atcagcgtgg gttgccagca gcgagccgtt gcccggctgc gacaggccca gcgcttcggt
    31021 caggcagttc attgagttag cagtaaacat cccggagcag gaaccgcagg ttgggcaggc
    31081 ggatcgttca acctgagcgc tctgggagtc agaaactttc gggtctgcgc cctggatcat
    31141 cgcatcaacc agatcgagct taatgatctg atcggacagt ttggttttcc cggcctccat
    31201 cgggccgccg gaaacaaaga tcaccggaat attcaggcgc agggaagcca tcagcatccc
    31261 cggggtgatt ttgtcgcagt tagagatgca gaccatggcg tcggcgcagt gggcattgac
    31321 catatactca acggaatcag cgatcagttc gcgagatggc agtgaataaa gcatcccccc
    31381 gtggcccatg gcaatcccat catccaccgc aatggtgttg aactctttgg caacgccgcc
    31441 cgccgcttca atttgttcgg cgaccagttt accgagatcg cgcagatgga cgtgacccgg
    31501 tacaaattgg gtgaacgagt tcacaaccgc gataatcggc ttaccgaaat cggcgtcggt
    31561 cattccggtg gcgcgccaca gcgcacgagc acccgccata ttacgaccat gagtggtggt
    31621 ggcggaacgg tacttaggca tactttattt actcccagtg tctgtctcgt aaatgggacg
    31681 gtgcgtgccg tcccattttt tgtatttatt gattaacttg atctaaccag ccccatttat
    31741 cttcggtttc gccagtgaag aggccgaaga aggcttgctg aatgcgtttg gtaaccgggc
    31801 cacaacggcc ttcgccaacc tgaataccgt ctacgctgcg cactggcgtg atttctgccg
    31861 ccgtaccgga cataaacact tcatccgcca ggtacaggga ttcgcgcgac agcacctgct
    31921 cacgcacttc aattcccagc tcttttgcca gtttgatgat ggcatcacgg gtaatacccg
    31981 gcagcgcgga tgaggtgaac ggtggggtga acagcacgcc gtctttcact tcaaacaggt
    32041 tttcgcctgc gccttcagag atgtaaccat tcacatccaa cgcgatacct tcctgataac
    32101 cgtggcggcg cgcttcgcta cccaccagca gggaagagag gtagttacca ccggcttttg
    32161 ccgccgtcgg gatggtgttt ggtgctgcgc ggttccagga ggaaaccatc gcatcgatcc
    32221 cctgctccag cgcttctgcg cccagatacg ctccccacgg gaaagcggcg ataatcacgt
    32281 cggttgagta tcccgctggc gggtttacgc ccatgccaac atcaccaacg aagatcagcg
    32341 gacggatata ggcgctggtg agattgtttt tgcggatcac gtcacgacaa gcttccatca
    32401 gctcatcaat gctctgcgaa accgggaagc gatagatttt ggcggagtca tgcagacgct
    32461 gcatatgctc acgatggcgg aatacaaccg gtcctttgtg cgagtcgtag caacggatgc
    32521 cttcaaaaac cgaggtgcca tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt
    32581 cccagcgaac catctcccca ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta
    32641 tattcctttt gcgctcaggc gcggatttgt tgtgatgtgg ttgtgctctg gcagatagca
    32701 acgtgtgcga cgtccaccag tttatttaac tgactaaaca gtaagtcgac cgaccgtggg
    32761 ctggcaacgg tcaattcgat atttatattt tgtgcatcgc tggcggcggc catattcatt
    32821 gagcagacgt ggaaaccacg atgacgcacc acgcgtaaaa cacgttctaa ggtttccgga
    32881 ttgaagcgag ccgatacatt gacctgatgt tgcatcatga taatttctcc aacatttcag
    32941 tattactggc accaggcggc accagcggcc agacgttctc aagttcgtcg attgagacat
    33001 gaagcaggta tggcccatca ctgttcagca tggtgtcgag tgccgcttca acctggtctt
    33061 tatgggtgat gtgttggcca gggatgccga aggcgctggc taacatgagg aaatcggggt
    33121 tatcggtaag ggtggtttcg ctgtatcgtt cctggaaaaa cagttgctgc cattgtcgaa
    33181 ccatccctaa ccgttggtta tcgagtaaga cgattttcaa cggtaactgc ttgcgtttta
    33241 cggtgcccag ctcttgcaca ttcatcatga aagagccgtc accggagata cagacgacag
    33301 tatcgttcgg tcgcgcgact tgtgcgccaa ccgccgccgg taaaccgaaa cccatggtgc
    33361 ctaagccgct ggaggtgatg aagttttccg ggcgagtgtg gacgatgtgc tgggcagccc
    33421 acatctggtg ctgccccaca tctgtggtca cgatgcaatc cgcagattta cgatccgaca
    33481 gttgttttaa caacaacggc gcgtagatag cgtcaccagg atggtcgtaa cgccaggcat
    33541 gttcatcacg cagttgcgcg cagtgttgct gccagtcatc gatatttaac ggctgctgta
    33601 atgctggtaa cagagcattt aaatcaccct gtaatgccac atgtgcctga cgcagcttgt
    33661 tcatttctgc cggatcgata tccatatgga taacgctggc gtgtggtgcg aaggtgttca
    33721 gtttgccggt cacccggtca tcaaaacgtg cgcccacggc gatcagtaga tcgcactcct
    33781 gcactgcgaa gttcgccgct ttggtgccgt gcatccccag catgcccaga tagtacggat
    33841 aatctgcttc tactgcgccc agccctttca acgtacaggt ggcaggcatt tttgtggcag
    33901 cgagaaattc acgcaaagcc gaaactgcct gcgccatgcc cacgccaccg ccaacgtaca
    33961 gcatcggttt ttgcgctttt gccagcatct gacgcgcttg ctcgacttcg gcatgtggga
    34021 aagtcacttc gttttcaacg gtggtgaacc acggttccag atcgccgctg gctaactgga
    34081 tatcttttgg aatatcgacc aaaaccggac caggacgacc agagctggcg acgtcaaacg
    34141 cttcggccat gatacgcggt aactcttcca gtgattgcac caggaaactg tgcttggtgc
    34201 aggctaacga caaccccaga atatccacct cctgaaatgc gtcagtgccg ataaacggtg
    34261 cggatacctg accggtgata gcaacaacgg ggatggaatc taacagcgcg tccgcaagcc
    34321 cggttatcag gttggttgca cctgggccag acgtggcgat gcaaacgcca gttttgccgg
    34381 tagcacgggc ataaccgata gccgccattg ctgcaccttg ctcgtgtcgg cacagcaagt
    34441 gttccacgcc accgtcatac aatgcatcgt aaaccggcat aattgcgcca cccggataac
    34501 cgaaaacggt gttcacaccc tgtgcccgca acgcatgtac cacccactgt gcgccattca
    34561 tagttagttc cccgtcctga atcttgagaa acagaatttt gtgctgttat tcattgtctg
    34621 ctcctcagtt atgtttttaa ggtcaaaaaa aacccccgga cctttcggtg cgggggtctt
    34681 agttcgttaa ggcttgatct ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg
    34741 ataataatca ccaccacgct aatcacgacc aggctaatca ctcgtagaag ggctgtcatt
    34801 ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga gttaccatag attttgcgaa
    34861 tagcacaaga tattttttgg ccgattttta tg
//
LOCUS       DADTFG010000036        34250 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.036, whole genome shotgun sequence.
ACCESSION   DADTFG010000036 DADTFG010000000
VERSION     DADTFG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.036"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(123..401)
                     /locus_tag="J1138_001612"
     CDS             complement(123..401)
                     /locus_tag="J1138_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453421.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(760..1050)
                     /locus_tag="J1138_001613"
     CDS             complement(760..1050)
                     /locus_tag="J1138_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8453422.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1051..1362)
                     /locus_tag="J1138_001614"
     CDS             complement(1051..1362)
                     /locus_tag="J1138_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA8453423.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1591..2499
                     /locus_tag="J1138_001615"
     CDS             1591..2499
                     /locus_tag="J1138_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA8453424.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATGHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2563..3504)
                     /gene="fabY"
                     /locus_tag="J1138_001616"
                     /gene_synonym="yiiD"
     CDS             complement(2563..3504)
                     /gene="fabY"
                     /locus_tag="J1138_001616"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBA8453425.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3549..3986)
                     /gene="dtd"
                     /locus_tag="J1138_001617"
     CDS             complement(3549..3986)
                     /gene="dtd"
                     /locus_tag="J1138_001617"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBA8453426.1"
                     /translation="MIALIQRVTRASVTVEGEVTGKIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3983..4855)
                     /locus_tag="J1138_001618"
     CDS             complement(3983..4855)
                     /locus_tag="J1138_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBA8453427.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4849..5448)
                     /gene="yihX"
                     /locus_tag="J1138_001619"
     CDS             complement(4849..5448)
                     /gene="yihX"
                     /locus_tag="J1138_001619"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBA8453428.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5547..6332)
                     /locus_tag="J1138_001620"
     CDS             complement(5547..6332)
                     /locus_tag="J1138_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8453429.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6366..7262)
                     /gene="yihV"
                     /locus_tag="J1138_001621"
     CDS             complement(6366..7262)
                     /gene="yihV"
                     /locus_tag="J1138_001621"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBA8453430.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQMLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7430..8326
                     /gene="yihU"
                     /locus_tag="J1138_001622"
     CDS             7430..8326
                     /gene="yihU"
                     /locus_tag="J1138_001622"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBA8453431.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNTEAVQHLVDKG
                     ATPAANPAQAAKGAEFIITMLPNGDLVRHVLFGENGVCESLSSDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8350..9228
                     /gene="yihT"
                     /locus_tag="J1138_001623"
     CDS             8350..9228
                     /gene="yihT"
                     /locus_tag="J1138_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBA8453432.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNEMCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9244..10485
                     /gene="yihS"
                     /locus_tag="J1138_001624"
     CDS             9244..10485
                     /gene="yihS"
                     /locus_tag="J1138_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBA8453433.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            10552..11454
                     /locus_tag="J1138_001625"
     CDS             10552..11454
                     /locus_tag="J1138_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBA8453434.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRGWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            11515..12390
                     /locus_tag="J1138_001626"
     CDS             11515..12390
                     /locus_tag="J1138_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBA8453435.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            12496..14532
                     /gene="yihQ"
                     /locus_tag="J1138_001627"
     CDS             12496..14532
                     /gene="yihQ"
                     /locus_tag="J1138_001627"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBA8453436.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDHGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14578..15963
                     /locus_tag="J1138_001628"
     CDS             14578..15963
                     /locus_tag="J1138_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8453437.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            16006..17409
                     /locus_tag="J1138_001629"
     CDS             16006..17409
                     /locus_tag="J1138_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8453438.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDATP
                     TGHKASILKSFYAIFSNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINL
                     RKNKEIETEEKTASVTL"
     gene            17477..18169
                     /gene="ompL"
                     /locus_tag="J1138_001630"
     CDS             17477..18169
                     /gene="ompL"
                     /locus_tag="J1138_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBA8453439.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(18271..20094)
                     /gene="typA"
                     /locus_tag="J1138_001631"
     CDS             complement(18271..20094)
                     /gene="typA"
                     /locus_tag="J1138_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBA8453440.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20467..21876
                     /gene="glnA"
                     /locus_tag="J1138_001632"
     CDS             20467..21876
                     /gene="glnA"
                     /locus_tag="J1138_001632"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBA8453441.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22050..23099
                     /locus_tag="J1138_001633"
     CDS             22050..23099
                     /locus_tag="J1138_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HBA8453442.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23111..24520
                     /gene="glnG"
                     /locus_tag="J1138_001634"
     CDS             23111..24520
                     /gene="glnG"
                     /locus_tag="J1138_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBA8453443.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24632..24742
                     /gene="yshB"
                     /locus_tag="J1138_001635"
     CDS             24632..24742
                     /gene="yshB"
                     /locus_tag="J1138_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBA8453444.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(24975..26348)
                     /gene="hemN"
                     /locus_tag="J1138_001636"
     CDS             complement(24975..26348)
                     /gene="hemN"
                     /locus_tag="J1138_001636"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBA8453445.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RKVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26537..27046)
                     /gene="yihI"
                     /locus_tag="J1138_001637"
     CDS             complement(26537..27046)
                     /gene="yihI"
                     /locus_tag="J1138_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBA8453446.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            27628..28260
                     /locus_tag="J1138_001638"
     CDS             27628..28260
                     /locus_tag="J1138_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBA8453447.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28642..31428)
                     /gene="polA"
                     /locus_tag="J1138_001639"
     CDS             complement(28642..31428)
                     /gene="polA"
                     /locus_tag="J1138_001639"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBA8453448.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEDIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            31430..31672
                     /locus_tag="J1138_001640"
     CDS             31430..31672
                     /locus_tag="J1138_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453449.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMVFVFVFHAVKNKAIPNN"
     gene            31792..32724
                     /locus_tag="J1138_001641"
     CDS             31792..32724
                     /locus_tag="J1138_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBA8453450.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSSFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQEK"
     gene            complement(32765..34195)
                     /locus_tag="J1138_001642"
     CDS             complement(32765..34195)
                     /locus_tag="J1138_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBA8453451.1"
                     /translation="MIRKSATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDMK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMLQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8228 a         8765 c         8479 g         8778 t
ORIGIN      
        1 cttcatttgc ttcatcagga tggtgtaccc gaaaaagtca gcagctttac cgcaccaggg
       61 tttaaggtga aatctaccat tcataacgac cggtcagtgc cgcaccaacc ggtctttaat
      121 tgttagtagt agtaaatttg tggataacag tccgggaacc agacgattcc ttccggatca
      181 tccccctgca ttacccgatg acattggcgt cgatcatctg cgcgacggcg aagcgtagta
      241 tagagccgct tccattcatg atcgcccggt gtactgtagt ttacccaaca atccattgaa
      301 gggtgacgcc atttacctgt aagtgttggg atccctgaat gcttatgcgg cttcgcttta
      361 ccattacgat ccctatcttt acgcgtatta aacgtggaca tttttagtct cctgttaaga
      421 gttctaaaaa tgtgcttcct taaaatagtt catcattttc ccttaattcg catagcgtgc
      481 ttttgcacga atttccgata cgcggggacc attatgatag cgtcgatgcc cacacatcca
      541 acaagaacaa atacagggag tacgaaagca aatacctgat gcttttttgc ttccatcgcc
      601 tgcagtgcga taacgacagc ggattttttt taatcgcacc atatgatggc gacgttcagc
      661 acgattacgg gtcatagcaa aactcctgag caggcggcac tatgccgctt tcataggagt
      721 tctgaatgct ggtgctttct gtatggcata accatgcctc taacggttaa gtgcttgcag
      781 cacatgttgc ggatcattgg caatcgctcg cagcaaagcc tttgcaggcc cggtcggtga
      841 acgtcgcccc tgctcccagt tacgcaacgt gtctacgctt acggaaataa gatcggcaaa
      901 tttagattgt gacaaccctg aagcgcggcg tatttctttg attttcatag catcaacttc
      961 aaaagtccgt gaaggctgtc gcgttccttc aataatttca ttcatctggg ccatgctgtg
     1021 agtcaaccgc tcaaaaagtg ccttatccat ttaccacctc gtatttaaca accggagcat
     1081 tgctttttcc tggggagaca gatcatcttt aataccctta cgataaataa gcagtaatct
     1141 gatctcataa tgtttaacct gatgaaagta aataaccctg acaccagcac gtttcccctt
     1201 tcctcctgag acccatcgaa cctttcttaa cccaccagtt tcaggtatta cctcaccata
     1261 gtcaggattc aaggccagga aaaactgaaa acgactgaat tcatcgtcgt tcagcagttt
     1321 ttgaacatct tcagtaaaaa tttccgtttc gatgaagagc ataattatac gccattggct
     1381 tagagtaaca atcctttttg tacgtcattg acgtaccatt gaaaccacta gcttactgat
     1441 agtgctccag caaaaccacg tctctgccgg gtttgtcttt acatttgcga ggcgctttcc
     1501 agatttttca cgcccgtgaa tttccctgta tccatccagc aaaaacgggt gtataaaact
     1561 gtacattcat acagcaaaat gagttctact atggcactgg aaaaaggaat tgcagagtta
     1621 gtcgaagagt ttatcgctgc gggtcgcccc tcatcgcgcc aacaaagtat tacgcagcgc
     1681 agagagggct atatcgccag cgctgtcctt gcgggtgaga cagaaacgcg ggtagatatt
     1741 cagactatcg aacttgaagg gatgacctta cgaatagtgt caccgctgaa tgccccgaca
     1801 ttactcccga cgataattta ttactacggt ggctgttttg ttagcggcgg gttcgcaact
     1861 cacgacaacc agctgcgcca gttagcctat tacgggcaat gtcggattat tgccgtccag
     1921 tatcggctgg caccggaaca tactttcccc gccgcccatg atgatgcaca acgaggtgca
     1981 gaattagtcc ggcaacacgc cgaacgactc ggcgttgata aacaacgaat aactcttgcc
     2041 ggtgacagtg cgggcgggca tctggcgctg gtaactgctt tgcgattgaa gcgtgctggc
     2101 gagtggcaac cagcacaact catcttaatt tatcctatgc ttgatgcgac tgggcatttc
     2161 gaaagctaca tacgcaatgg tcacgattac atcatcacac gcgacacttt actgagcggc
     2221 tttgagatgt acctgccagg cattgaacgc aggcatcctg aagccagccc tatttggcgc
     2281 aatgatttca atggcttgcc acctgtacat atcattaccg ctgaatacga cccactttgc
     2341 gatgaaggtg aagcgttgta tcaccgcatg acagggcaag gagtgcaatg cacctgccaa
     2401 cgctaccttg gcgttatcca cggatttttt cagttaggtg gtatcagtga ggctgcgcga
     2461 tcggcgctac gggatgttgc ctggagagcg ggaaggtaac tccggaccag ggcccagaaa
     2521 aggttttcag attgaaaacc ttttcagtca agtaaaacgc ccctactctt cttcgttccc
     2581 gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc cttcaaacac
     2641 tgcacccggc gtctcgtcgc caaagatttc gacctgcatt tgtacccgcg cttttcgtcc
     2701 gcgcgccaga cggtcgagat cgccacttaa ggcaccaagg tcggctaccg catggggttt
     2761 accactaata ggtttgctat agcggatatg cgcatccgcc agaataatcg ttccgccgag
     2821 gtggcgttcg cgcagcatca gccagataag cccccaaccg gtgagcgtag ccagcgagaa
     2881 taaactcccg gcaaacagcg tgtgatgcgg attctgattg ccggtttccg gcatggtggt
     2941 gataaatttt tgcccggtat attgctgaat gcgcacaccc attttttcgc ttaacgggat
     3001 atgttcatac cacgcttgct ggagctgggc gcaccagtcg ccgcgatgaa gaatatcatc
     3061 cagagtggcg acgggtttaa tcatcagaaa atggcgaatc ggcgtggtgg ttggcgtggt
     3121 gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt cttcacgggc
     3181 gctacaggtc acgcgcttaa cgccttcctg gcgcgctacc gactccaggg tcatcgccat
     3241 cagcgttccc agccctttgt cctgcacgtc aggatgaacg gccataaagc gaatggacgc
     3301 ttcattgtcg gcattaatat acagtcggcc taccgctacc agattaccct gctcgtcgac
     3361 gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt ttggttgatg
     3421 caggggctta cgcaacattt cccagcgaaa ctgatagtaa cgctctaatt cttcttctgt
     3481 ttgtggaacc cgaaggtgat acatagcgat actctctctt gtttcccgtg acaaccctgg
     3541 aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag cgatacctgc
     3601 atatccgcag cgaagcgtcc tgtttgcgtg ttcatctctt gctgacggca gcgttcgacg
     3661 aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa acttgggcgc
     3721 atcccccgtt cggtatctgc ggcaagggta aactgggaaa ccaccagtac actgccgccc
     3781 gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg gtagccgagc
     3841 acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac acccaataac
     3901 accaaaagtc ccgcgccaat tttacccgtc acttctccct ccacggtgac gctggcacgg
     3961 gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca gcagcttgtt
     4021 ttaatttgcg gtattccccg agagtgacag taatttccgc gccaagcaag acgatacacc
     4081 atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg taaatgagct
     4141 gatatgatgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct tcgaacagga
     4201 gcgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacacggatg gtaggaacga
     4261 tgctgtacag cagccagaaa gagatccacg acaacagcag cggaaaaata cgcaacacat
     4321 tatcgatgac agtattgaga tcgctcgccc agcgcagaga gagcagatag gagctgatcg
     4381 ccagactggc ccctgccaac agcggcccca gcgttaaaat catccagtac acggcaaacg
     4441 agtaaatttt gggtcgcgct cgtttactgc gccagatggt attcaacgcg ctatcgatgg
     4501 agtacatcaa caataacgcc gtgacgatca gcccgcacgc cccgacggcg gtcattttgt
     4561 tggaattggc gacaaactgt tcgatatatc gctggataac atcgccagta gcaggcagaa
     4621 agttggcaaa aataaagtga cgcaactgaa tgctgacgtc ggaaaacatg ggaaaagcgg
     4681 cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac acataggcaa
     4741 ggttacctgc cagggttgtc atgttgtcct catcaatgcg ttgccagagt agttttagcc
     4801 aggcccatag tggacgggta cgatgcctgg ctttgtcctg aatggttttt agcataacac
     4861 cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg taatgcctag
     4921 ccgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat cgtctggtga
     4981 aaaaccttct gcctgcaaaa catgctgata aattcgtgct tcaggtttgc gcatacccaa
     5041 gtcttgcgac agatagatat ggtcagcagc atcacggatt tccgggtatt cctccggcca
     5101 gaaggtagta tgcaggcggt tagtattgga aagcaccacc acgcgatgcc cctgctcacg
     5161 cagtttatgc atgatggcga tcacttccgg tcgtagcgca acaaataccg cctgccaacc
     5221 gtgggagaac tgctcatagc ttagcggcag ggccatctca tgacacagtg cctctgcgaa
     5281 cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctcgccca tgtggaaact
     5341 cttcttaagc gtagccagtg gaacacgcgt taaatcgctc caggccccca gcacacggtt
     5401 aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt ttgcctcctt
     5461 tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt ggccaggtgt
     5521 gaagccaggt caacggtcac aaaattctac gcgtcttcct ggggaaggat gatttcaatt
     5581 tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt aaagagcgca
     5641 gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt agtgtgatcg
     5701 gcgaccagca ggatatttct cgcgtgcgcc atcatcgttt tcaccacgtt agcttcgtta
     5761 acatcaaact ccatcaacgc gccatcgctc tcgattgcgc ctacgcttgt taccagataa
     5821 tcggcgcgaa aatcggccac aaaggacgcc gctgaagggc caatgatccc actgttatgg
     5881 gagcgcagcg ttccgccagg caccatcact tcaaagcgcg ggttgtggta aagaatatgc
     5941 gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa cgcccgggca
     6001 acatgctcaa cggtcgtacc aatggtgata aatattgttg aaccatcagg aatataatct
     6061 gccaccgctt cggcaatcgc ttttttttct tcggtttgcg aaacctcacg ctgctcgaac
     6121 gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt aatcagtcct
     6181 tgctcgctta atttacgaat atcccggcgc accgtctgcg tggagacatc cagcagattt
     6241 gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag ttggtcgtgc
     6301 cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac catcaccctg
     6361 gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg gatcccggct
     6421 cgtccaccgg gacgcgtgca tttcaacgcc gctactccgc tggcgaagcg gactgattcc
     6481 gctaaatccc cacctgttgc cagcgccacc gccaatgcgc cgtgaaaaac atcacccgca
     6541 cctgtggtat ctaccacatc aactttgaag gccggttgat gctgtcgccc gccattctcc
     6601 agccagtcac agcctgcact gccctgggtc acatagacat gaccatttgt gagcatttgt
     6661 gcctgtttta gggcactcgc catctctttc acgcccgtta agcgcgccag acccggttct
     6721 gaaaaagctg catgatcgct taacgccacc agttcgctga tatcctgcgg cgtaatatcg
     6781 ccgtccagca cggtcatcac gcccgcctgc cgcgccagag tgaaggcttt tttcgcgccg
     6841 tcgtgccagc gtacatctgc cagcaccacg tcccactgag agaaatcaat ttcctccaac
     6901 cactctgcat caggcagcag gtctgggctg ggatagttaa tgatgatgcg ctcacctttg
     6961 gcatccacca tgatggcgga ttgcgaagat ttcgcctggt tataccgttt ggtgtaacgg
     7021 gtgttaacac cccaggattc cagctctgcc agcaggctgt tgcccgtgtc gtcgtcaccg
     7081 acgcgaccaa taaaatcgac ctgcgcacct agtctggccg ccgcaactgc tgctgtagcc
     7141 gctggcccgc cgccaacttc cgtataattt ttcgccacgt atttaccgct ctccgttggc
     7201 aatccttcca cgtaatagat gcgatccatc acggtaattc ctacacaagc aacacgaatc
     7261 atggtcattc cttaagcatt ttgggatgcg ttcattttaa tttcatcaat tgtttaaaaa
     7321 gtgacggctg tcaatttttt gacgataaat tacaaatacg atcaaaaaca gacaagcaag
     7381 gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagagtta tggcagcaat
     7441 cgcgtttatc ggtttagggc aaatgggttc gccaatggcg agcaatttat tgcagcaagg
     7501 gcatcaactt tgcgtcttcg atgtgaatac cgaagccgta cagcatctgg tcgacaaagg
     7561 ggcgactccc gctgctaacc cagcgcaggc cgctaaaggt gccgaattta tcattaccat
     7621 gttgccgaat ggcgatctgg tgcgtcacgt gttgttcggt gaaaacggtg tctgtgagag
     7681 cttatcttcc gatgcgctgg tcattgatat gtcgaccatc cacccgctgc aaaccgataa
     7741 attgattgct gatatgcagg ccagaggctt cagcatgatg gatgttccgg taggccgtac
     7801 ttccgcaaat gccattaccg gtactctgct actgctggct ggcggcactg ctgaacaagt
     7861 tgaacgcgcc acgccgatcc tgatggcgat gggcagcgag ttgatcaacg cgggcggtcc
     7921 gggcatgggg atccgcgtta agcttatcaa caactacatg agcatcgcgc tcaatgcgtt
     7981 gtcggcagaa gccgccgttt tgtgcgaagc cctcaatctt cccttcgatg ttgccgtcaa
     8041 ggtaatgagc ggtactgccg ccggtaaagg ccacttcacc acttcctggc cgaacaaagt
     8101 cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg atcttggcat
     8161 cgcccttgat gtcgccaacc agttgcatgt gccgatgcca ctgggggccg cctcacggga
     8221 ggtttatagc caggcgcgcg cagcgggtcg cggtcgccag gactggtccg ccattctgga
     8281 gcaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac tggataaagg
     8341 aataaatgaa tgaataagta caccatcaac gacattacgc gtgcatcggg cggttttgcc
     8401 atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc tggcgcaccc
     8461 gctccagtag ccgatagcgt tttaactgat ttcaaagtta acgccgcgaa gactctttca
     8521 ccttatgcat cggcgattct ggtagatcaa caattctgct accgccaggt ggttgagcaa
     8581 aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat tcctggcaac
     8641 ggtattccgg tagatagcgt ggtcattgac cgcaaaatca atccgctaca aatcaaacaa
     8701 gacggtggca aagccttaaa actgctggtg ctgtggcgta gtgatgaaga tgcgcagcaa
     8761 cgcctggata tggtgaaaga gtttaacgaa atgtgccact cacacggtct ggtaagcatc
     8821 attgagccag tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga acaagcaatc
     8881 atcgatgcag ccaaagagct gggcgacagt ggcgctgatc tctacaaagt tgaaatgccg
     8941 ctttacggta aaggtccgca acaagagctt ctcagtgctt cacaacgtct gaatgaccat
     9001 atcaatatgc cgtgggtgat cctctcttcc ggtgtcgacg aaaaactgtt cccgcgtgcc
     9061 gtacgcgtgg caatgacagc gggtgcatcg ggcttcctgg ctggtcgtgc cgtctgggca
     9121 tcggtcgtcg gtttaccaga caacgagctg atgctgcgtg acgtttgcgc accgaaatta
     9181 caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgttaaga tggggataca
     9241 aaaatgaaat ggtttaacac tttaagtcac aaccgttggc tggaacagga aaccgatcgc
     9301 atctttgatt ttggtaaaaa atccgtagta ccgaccggat ttggctggtt aggcaataaa
     9361 gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat gttgcacgtt
     9421 tattccgttg ctgcggcgat gggccgacct ggcgcttact cgttggttga tcacggtatc
     9481 aaagccatga atggcgcact gcgcgataaa aaatatggcg gttggtatgc ctgcgtgaat
     9541 gacgagggcg tggtggatgc ctccaaacag ggctatcaac atttctttgc tctgctgggt
     9601 gctgccagcg ccgtcactac cggtcatccg gaagcgcgca agctgctcga ttacaccatt
     9661 gaaattatcg agaaatactt ctggagcgaa gaagagcaga tgtgcctgga gtcctgggat
     9721 gaagccttca gcaaaaccga agagtatcgc ggcggcaatg ccaatatgca cgcggtggaa
     9781 gccttcctga ttgtctatga cgtcacccat gacaaaaaat ggctggatcg cgcgattcgc
     9841 gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt taacgaacac
     9901 ttcgataccc agtggaatcc gctgccagat tacaacaaag ataacccggc gcaccgcttc
     9961 cgcgcgttcg gaggtactcc gggccactgg atcgaatggg gccgtttaat gctgcacatt
    10021 cacgcggccc tggaagcccg ttgcgaacaa ccgccagcat ggctgctaga agatgccaaa
    10081 ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggcgcgga cgggattgtt
    10141 tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtgcgttg gcctatcgtc
    10201 gaagcgatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca gtacgaaacc
    10261 tggtatcaga catggtggga ctactgcatc aaatacctga tggactacga aaatggttca
    10321 tggtggcagg agctggatgc ggacaataag gtcaccacca aagtctggga cggcaaacag
    10381 gatatttatc atctgctgca ttgtctggtg atcccacgta tcccgttagc gccaggcctg
    10441 gctccggcgg ttgcggcggg tctgctggat attaacgcga aataactgac taaaaaaacg
    10501 acatatcagg ctgcacgctg ggtatgtcgt tattcaataa aggtacctcg tatgcaaatc
    10561 accaacatgc attgcagtgg acagaccgtt tcacttgccg cgggggatta tcacgcgacc
    10621 attgtgaccg tcggtgcagg tctggctgaa ttaacctttc agggctgtca tttagttatt
    10681 ccgcataaac ccgaagagat gccgctggca catctgggca aagtccttat tccgtggcca
    10741 aaccggatcg ccaacggctg ttatcgctat caggggcagg aatatcagtt acccattaat
    10801 gaacatggtt caaaagccgc tattcatggg ctgctcgcct ggcgtggctg gcaaatcagc
    10861 gaattaagcg ccaccagcgt cacattaacc gcctttctgc cacccagtta cggctacccg
    10921 tttatgctgg cttcgcaggt ggtttattcg ctcaacgccc gcacgggtct ttccgttgag
    10981 atcgcaagcc agaatatcgg cactgtcgca gcgccttacg gtgtgggtat tcacccttat
    11041 ttaacctgca acttaacatc tgttgatgaa tatctgttcc agcttccggc aaaccaagtt
    11101 tatgccatcg atgagcacgc taatccgaca acgcttcacc acgttgatga gctggattta
    11161 aatttcacgc aggcgaaaaa aatagccgcg acaaaaatcg accacacctt taaaaccgct
    11221 aatgatttgt gggaaatgac tattacccat ccacagcagg cgctttcggt gagcctctgt
    11281 tccgatcaac cctgggtgca ggtttatagc ggtgaaaaat tacaacgtca gggactggca
    11341 gttgaaccaa tgagttgccc gcctaacgcc tttaattcag gtatcgactt attattactg
    11401 gaaccaggga aaacgcaccg cctgtttttt aatattcacg ggcagcacaa ttaattttat
    11461 taagaaaaga acgagatcat tgtttagagt tgtatttaca caacaggaaa tattatggac
    11521 ttttttacgt tattttcacc ggatggggtc atgctggaaa caaacgtggt atgtatcgtt
    11581 atcgcgttaa tgtttcttta tacctttgtg gggatatgcg cgggatttgg cggtggttta
    11641 accaccatgc cgctcgtcac gttaatgctc cccgtaaaaa tggcaactcc gctctcggtg
    11701 attgtcggca ccgcgaccgc cttgtacgcc acctggctgt cacgtaaaga aacggattgg
    11761 cgatcggcaa tggtactgat tattttctcg tttctgggaa tacccgtcgg tttatatgcg
    11821 ctgtcgtatt tacccgacca ttttatgaaa gtgggcttag gcggattcct tatcctgtac
    11881 tccttttaca gtatgtttat tccacgcctg ccgatttatg accgtagctg gatcgccgca
    11941 cctatcggcg ccgtggcagg agcgttaggg gcagcatttt caaccaacgg accgccgatg
    12001 gttatctacg gtatgctgcg taacttaggc ccggcggcat ttcgcggcac tctgaacgct
    12061 ttcttcaccg caaacaacat cgcggtgatc ggcgggctgg cgaccagcgg aattttgact
    12121 gtatctatct ttaaactggt gctgttttgt attcccacca tgatcctcgg ttcactggtt
    12181 ggacaatatg tgcataaacg catttcggtc aaagtgttcc gtgtgatggt ctttttattg
    12241 ctgattgcct ccggttctat gttgataaaa ggcgcgctgg gaattcccgc cgctgccgca
    12301 cttcttcctc cgtttacctt gttagttata ctgcaactgg catttggtaa aaaagtggcg
    12361 gcaatccgtc gggcggaaga aagtaaataa tcattatgcg ggttttaatt tatacccgca
    12421 cacattcttt aattaatgaa acctgtaata acaggatggc atgaaatatt agttttatat
    12481 ctatggagat tatttatgga tacgccacgc ccacagctat tagattttca atttcatcag
    12541 aataacgaca gttttactct acgttttcaa gaccgcctta ttttaatcca tagcaaagat
    12601 aatccttgtt tatggattgg ttcaggtata gcggatatcg atatgttccg cggtaatttc
    12661 agcattaaag ataaattgca ggagaaaatt gcgcttaccg acgccatcgt cagccagtca
    12721 ccggatggct ggttaatcca tttcagccgt ggttctgaca ttagcgccac gctgaatatc
    12781 tctgccgacg atcatggccg tttattgctg gaactacaaa acgacaacct taaccacaac
    12841 cgcatctggc tgcgccttgc cgcacagccg gaagatcata tctacggctg cggcgaacaa
    12901 ttctcctact ttgatctgcg cggcaaaccg ttcccgctgt ggaccagtga acaaggcgtt
    12961 ggtcgcaaca aacaaaccta tgtcacctgg caggccgact gcaaagagaa tgcgggaggg
    13021 gattactact ggaccttctt tccacaaccc acgtttgtta gcacgcagaa gtattactgc
    13081 catgttgata acagttgcta tatgaacttc gactttagcg cgccggaata ccatgaactg
    13141 gcgctgtggg aagacaaagc aacactgcgt tttgaatgtg ctgatacata catctccctg
    13201 ctggaaaaat taaccgcgct gctgggacgc cagccagaac tgcccgactg gatttatgac
    13261 ggagtaacgc tcggcattca gggcggcact gaggtgtgcc agaagaaact ggacaccatg
    13321 cgtaacgcgg gcgtgaaggt caacggcatc tgggcgcagg attggtccgg tattcgtatg
    13381 acctcgtttg gcaaacgcgt aatgtggaac tggaagtgga acagcgaaaa ctacccgcaa
    13441 ctggattcac gtatcaagca gtggaacaaa gagggcgtac agttccttgc ctatatcaac
    13501 ccgtatgttg ccagcgataa agacctctgc gaagaagcgg caaagcgcgg ctatctggca
    13561 aaagatgccg ccggcggcga ttatctggtg gagtttggcg agttctacgg cggcgttgtc
    13621 gatctcacta atccggaagc ctacgcctgg ttcaaggaag tgatcaaaaa gaacatgatt
    13681 gaactcggct gcggcggctg gatggctgac ttcggcgagt atctgcccac cgacacttac
    13741 ctgcataacg gcgtcagcgc cgaaattatg cataacgcct ggcctgcgct gtgggcgaaa
    13801 tgtaactacg aagcccttga agaaacgggc aagctcggcg agatcctctt ctttatgcgc
    13861 gccggttcca ccggtagcca aaaatattcc accatgatgt gggcgggcga ccagaacgtt
    13921 gactggagtc tggacgatgg actggcgtct gtcgtcccgg cggcgctgtc gctggcaatg
    13981 accggacatg gcctgcatca cagtgatatt ggcggttaca ccaccctgtt tgagatgaag
    14041 cgcagcaaag agctgctgct gcgctggtgc gatttcagcg ccttcacgcc gatgatgcgc
    14101 acccatgaag gtaaccgtcc tggcgacaac tggcagtttg acggcgacgc ggaaactatc
    14161 gcccattttg cccgtatgac caccgtcttc actaccctga aaccttacct gaaagaggcc
    14221 gtcgcgctga atgcgaagtc cggcctgccg gttatgcgcc ctctgttcct gcattacgaa
    14281 gacgatgcac agacctacac cctgaaatat cagtacctgt taggccgcga cattctggtc
    14341 gccccggtac acgaagaagg tcgcagcgac tggacgctct atctgccgga ggataactgg
    14401 gtccatgcct ggacgggtga agcgttccgg ggcggggaag tgaccgttaa tgcgcccatc
    14461 ggtaaaccgc cggtctttta tcgcgccgat agcgaatggg cggcactgtt cgcgtcgtta
    14521 aaaagcatct aatcacatcc gcccgtgggg aaactcacgg gcaaagggag aacaacgatg
    14581 agtcacatca caacggaaga tccagcaact ctacgcctgc cctttaaaga gaaactctct
    14641 tacggtatcg gcgatctggc ctctaacatc ctactggata tcggcacgct ttatcttttg
    14701 aagttttata ccgatgttct ggggctgcct ggcacctatg gcggcattat ctttttgatt
    14761 tcaaaattct ttacagcctt taccgatatg ggtaccggca ttatgttgga ttcccgacgc
    14821 aagatcggtc caaaaggtaa gttccgtcct tttattctgt atgcgtcatt tccggtcacc
    14881 ctgctggcga tcgccaactt tgtcggcaca ccgtttgatg tcaccggtaa aacggtgatg
    14941 gccaccatcc tgtttatgct ttacggactg tttttcagca tgatgaactg ctcgtatggc
    15001 gcgatggtcc ccgctattac taaaaacccc aacgaacgtg catcgctggc agcatggcgt
    15061 cagggtggcg ctacattggg cctgctgctg tgtacggtgg gattcgtgcc ggttatgaat
    15121 cttatcgagg gtaatcagca acttggctat atcttcgccg ccacgctgtt ttcactgttc
    15181 ggcctgctgt ttatgtggat ctgctattcg ggcgtgaaag agcgttatgt cgaaacccaa
    15241 cctaccaatc cggcgcaaaa gcctggcttg ttgcaatctt tccgcgcaat tgcggggaac
    15301 cgcccactgt tcattttgtg cattgccaac ctctgtacct taggggcgtt taacgtcaag
    15361 ctcgccattc aggtctatta cacccagtac gtgcttaacg atcccatcct gttgtcatat
    15421 atgggatttt tcagcatggg ctgtattttc atcggcgtgt tcctgatgcc tggtgcagtc
    15481 agacgttttg gtaagaagaa ggtctatatc ggcggtctgc tgatctgggt gctgggcgat
    15541 ctgctcaact atttcttcgg cggcggctcg gtcagctttg tggctttctc ctgcctggcg
    15601 ttctttggtt cggcgtttgt taacagcctg aactgggcgc tggtttctga caccgtcgag
    15661 tatggcgagt ggcgcaccgg tgtgcgttcg gaaggtacgg tctacaccgg tttcaccttc
    15721 tttcgcaaag tatctcaggc gctggcaggt ttcttcccag gctggatgct gacgcaaatt
    15781 ggctatgtgc caaacgtcgc ccaggctgac cacactatcg aagggttgcg ccaactgatc
    15841 ttcatctacc caagcgcact ggcagtagtc accattgtgg caatgggttg cttctacagt
    15901 ctgaacgaga agatgtacgt ccgcattgtt gaagagatag aagcccgtaa acgcacggcg
    15961 taattataat aaacaacgcc ctgcggggcg ttataaggag tgattatgtc tgaccataat
    16021 ccactgacat taaaactgaa tctgcgggaa aaaatcgcct atggtatggg cgacgtcggt
    16081 tcgaatttaa tgcttagcat cggcactctg tatctcctca aattttatac cgatgaatta
    16141 gggatgcctg cttactatgg cgggattatc tttctggtgg cgaagttttt taccgcattt
    16201 accgatatgc tcaccggatt tttactcgac tcgcggaaaa atattggacc taaaggaaaa
    16261 ttcaggccgt ttattttata cgcagcgatt ccggcggcgt taattgcaac gctccagttt
    16321 atcgccacca cttttagttt gccggttaaa acgacgattg ccaccgcgct gtttatgatg
    16381 tttggacttt catacagtct gatgaactgc tcgtatggcg cgatgatccc ggccattacc
    16441 aaaaacccga atgagcgcgc gcaactcgct gcttaccgcc agggtggtgc aaccataggg
    16501 ctattgatct gtaccgtggc gtttattcct ttgcagtcac tattttctga ctccaccgtc
    16561 ggctacgcct gcgcggcact gatgttctcc attggcggct ttatttttat gatgctgtgc
    16621 tacagaggtg tcaaagagca ttatgtggac gcgacgccaa ccggacataa ggccagtatt
    16681 ctcaaatcat tttacgcaat atttagtaat ccacctttgc tggtattgtg cattgctaac
    16741 ctgtgtaccc tggcggcatt taatatcaaa ctggcgattc aggtctatta cacccagtat
    16801 gtgctgaatg atattaactt attgtcgtgg atgggattct ttagtatggg atgcattctc
    16861 gtcggcgtat tactggtgcc actaactgta aaatgttttg gtaaaaaaca ggtttatctg
    16921 gctggcatgg tgctgtgggc ggtgggggat atcctgaatt attgctgggg cagtaactca
    16981 ttcactttcg tcatgttctc ttgtgtcgcc ttttttggca cggcctttgt aaacagcctg
    17041 aactgggcac tggtaccaga taccgtagat tatggtgaat ggaaaacagg tatacgtgcc
    17101 gaaggttctg tttataccgg ttataccttc tttcgtaaaa tttcagccgc acttgctggc
    17161 ttcttgccag gcattatgct gacgcaaatc ggttatgttc ccaacatcgc gcaaagcgat
    17221 gcaacattac agggtttgcg tcagctcatt tttatctggc cttgtgcgct ggcaattatt
    17281 gctgcattga cgatggggtt cttttacacg ctcaatgaga aacggtttgc actgattatt
    17341 gaggaaatta acctacgcaa aaataaagaa attgagacag aagaaaaaac ggcttccgta
    17401 acgttataaa tgtcaatagc cgctatttcc atcctgatgg gtggcggcct ccctacgttt
    17461 aaaaaatgga cttattatga aaaatattaa tgcaataatt ttattatctt ctctgacctc
    17521 tgcctcggta ttcgctggcg catacgtcga aaacagggaa gcatataatc tcgcctccga
    17581 tcagggtgag gttatgctac gtgtgggtta taacttcgat atgggcgcgg gtattatgct
    17641 aaccaatacc tacacctttc agcgagaaga tgaactcaaa catggctata atgaaattga
    17701 aggttggtat cctttattta aacctaccga taaattaacc atccagcccg gtggcttaat
    17761 taatgacaag agtatcggtt cgggtggtgc tgtttatctg gatgttaact acaaatttac
    17821 tccgtggttt aacctgacgg tgcgtaatcg ctataaccat aataattaca gctctacaga
    17881 tttgaacggc gaattggata ataacgacac ctatgaagtt ggcacctact ggaactttaa
    17941 aataacagaa aaattttcct atacatttga accacattac ttcatgcgag tgaatgactt
    18001 taatagtagc aacgggaaag atcatcattg ggaaatcact aacaccttcc gttaccgtat
    18061 taatgaacac tggcttcctt atttcgaatt gcgctggtta gaccgcaacg tcgagccgta
    18121 ccaccgcgag caaaaccaga ttcgtatcgg aacgaagtat ttcttctgat gtaaaaattc
    18181 ctgatgcgat gctggcgcat cttatcaggt ctacaaaagc caaaaaaaac ctgccatcgc
    18241 tggcaggttt tttagtcata aaaaacgaaa ttaatcgtct ttcggtgcgc ggttggcgcg
    18301 acgacgatcg ttttccgtca ggtgacgttt acgaatacga atagaggtcg gagtcacttc
    18361 taccagttcg tcatcatcga tgaactccag agcttgttcc agagtcatgc ggataggcgg
    18421 aaccagaaca acggcttcgt cagtaccgga agcacgcatg ttggtcagtt tcttaccggt
    18481 caggcagttt acagtcaggt cgttagagcg gctgtgaata ccgataatct gaccttcgta
    18541 aacttcagca ccgtgaccga ggaacagctt accgcgatcc tgcagaccga acagcgcgaa
    18601 cgcgaccgct ttaccctgac cgttagagat cagtacgccg ttctgacgct gacccacttc
    18661 acccggacgt acgtcgtcgt agtggctgaa ggtggagtac agcagacccg taccggaagt
    18721 catggtcatg aactcagaac ggaagccaat cagaccacgg cttgggatca cgtagtcgag
    18781 acgtacgcgg cctttaccgt ctggattcat gtttttcagg tcgcctttac gttcgcccag
    18841 cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc agcgtcacgt tttcatacgg
    18901 ctcttgttta cgaccgtcga tttcacggaa gataaccttc ggacgggata ccgccagttc
    18961 gaaaccttca cgacgcatgt tttcgatcag aacagacagg tgcagttcgc cacgaccaga
    19021 aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc agcgcaacgt tgtgtaccag
    19081 ttctttgttc agacgatcca ggatctgacg agacgttacg aacttacctt ctttaccgca
    19141 gaacggcgag gtgttaacgc agaagaacat agaaacggtc ggctcatcaa cggagagtgc
    19201 cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca gttcgccaag
    19261 acccgtgatc gccacgatat cgccagcttc cgccagatcg gtttcgatac gttccagacc
    19321 gaggtggccc agcactttac cgactttcgc gttgcgggtt ttgccttcgc tatcgatgat
    19381 agtaacctgc tggttcggct tcactttacc gcgcttgatg cggccaatgc cgataacgcc
    19441 aacatagctg ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt caaggtcaac
    19501 gtccggcgca ggaacgtggt caacaatcgc ctggtacagc ggggtcatgt cttccgccat
    19561 atcttcgtgg tccagacccg cgataccgtt cagcgcagaa gcgtaaacga tcgggaagtc
    19621 cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg aatacctgat ccacaaccca
    19681 atcaggacgc gcgccagggc ggtcaacttt gttgataaca acaatcggct tcaggccgta
    19741 agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa atgcgtcaac
    19801 caccagcagc actgagtcta ccatggacat tacacgttca acttcaccac cgaagtcggc
    19861 gtgccccggg gtatcaacga tgttgatacg gtaatcattc catttgatag cggtgttttt
    19921 cgcgaggatg gtaatcccac gctctttctc caaatcgttg gagtccatca cgcgctcttg
    19981 ggtttcggca cgagagtcga acgtaccgga ttgttggagc agcttgtcta ccagggtggt
    20041 tttaccatgg tctacgtgcg cgatgatggc gatattacgc aatttttcga tcacaacttt
    20101 gcctcaggca ttagaaatag cgcgttattg tacacggatt aatcgcacta caaaacagga
    20161 tcacaaacat cctccgcaaa caagtattgc agagtccctt tgtgatcgct ttcacggagc
    20221 ataaaaaggg ttatccaaag gtcattgcac caatatggtg cttaatgttc ccattgaagc
    20281 actatattgg tgcaacattc acatcatggt gcagcccttt tgcacgatgt taagcatgat
    20341 aacgcctttt aggggcaatt taaaagttgg cacagatttc gctttatctt ttttacggcg
    20401 acacggccca tttaattgca gatttcgtta ccacgacgac aatgaccaat ccaggagagt
    20461 taaagtatgt ccgctgaaca cgtactgacg atgctgaacg agcacgaagt gaagtttgtt
    20521 gatttgcgct tcaccgatac caaaggtaaa gaacagcacg tcactatccc tgctcatcag
    20581 gtgaatgccg aattcttcga agaaggcaaa atgtttgacg gctcctcgat tggcggctgg
    20641 aaaggcatta acgaatccga catggtgctg atgccagacg catccaccgc agtgattgac
    20701 ccgttcttcg ccgattctac cctgattatc cgttgcgaca tccttgaacc tggcaccctg
    20761 caaggctatg accgtgaccc gcgctctatt gcgaagcgcg ctgaagatta cctgcgttct
    20821 actggcattg ccgacaccgt actgttcggg ccagaacctg aattcttcct gttcgatgac
    20881 atccgtttcg gatcgtctat ctccggttcc cacgttgcta tcgacgatat cgaaggtgca
    20941 tggaactcct ccacccaata tgaaggtggt aacaaaggtc accgtccggc agtgaaaggc
    21001 ggttacttcc cggttccgcc ggtagactca gctcaggata ttcgttctga aatgtgtctg
    21061 gtgatggaac agatgggtct ggtggttgaa gctcatcacc acgaagtcgc taccgcaggt
    21121 cagaacgaag tggctacccg cttcaatacc atgaccaaaa aagctgacga aattcagatc
    21181 tacaaatatg tcgtgcacaa cgtggcgcac cgtttcggta agaccgcgac ctttatgcca
    21241 aaaccgatgt tcggtgataa cggttccggt atgcactgcc acatgtcgct gtctaaaaac
    21301 ggcgttaacc tgtttgctgg cgacaaatac gcaggtctgt ctgaacaggc gctgtactac
    21361 attggcggcg taatcaaaca cgctaaagcg attaacgccc tggcaaaccc gaccaccaac
    21421 tcttacaagc gtctggtccc gggctacgaa gcaccggtaa tgctggctta ctctgcgcgt
    21481 aaccgttctg cgtctatccg tattccggtg gtttcttctc cgaaagcacg tcgtatcgaa
    21541 gtacgtttcc cggacccggc ggctaacccg tacctgtgct ttgctgccct gctgatggcc
    21601 ggtcttgatg gcatcaagaa caagatccat ccgggcgaag ccatggacaa aaacctgtat
    21661 gacctgccgc cagaagaagc gaaagagatc ccacaggttg caggctctct ggaagaagca
    21721 ctgaacgaac tggatctgga ccgtgagttc ctgaaagccg gtggcgtgtt cacggatgaa
    21781 gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg tatgactccg
    21841 catccggtag agtttgagct gtactacagc gtctaatagt tgaagttgta ctacccggcg
    21901 caacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt
    21961 tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa
    22021 atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc
    22081 agatcctcaa ctcgctgatt aacagtattt tgttaatcga tgacaacctg gcgatccatt
    22141 acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacac
    22201 cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg
    22261 aggcggggca aggttttacc gataacgaag tgacgctggt catcgacggg cgctcgcata
    22321 tcctttctgt gacggctcag cgtatgccgg acggcatgat cctgctggag atggcaccga
    22381 tggataacca gcgccgctta agtcaggaac agctacagca cgcccagcag gttgctgccc
    22441 gtgatttagt gcgcggcctg gcgcatgaga ttaaaaatcc gcttggcggt ttacgtggcg
    22501 cggcgcagct actcagcaag gcattacctg acccgtcact actcgaatat accaaagtga
    22561 ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg tctgttgggg ccgcagctgc
    22621 ccggtacgcg tattaccgaa agtattcaca aagtggctga acgcgtggtg acgctggtgt
    22681 cgatggaact gccgaacaac gtgcggttga ttcgggatta cgaccccagc ctgccggaac
    22741 tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc aatgcgctac
    22801 aggcgctggg gccggagggt ggtgaaatca ttctgcgtac ccgcacagcg tttcaactga
    22861 ccttacacgg cgagcgctat cggctggcgg cgcggattga tgtggaagat aacgggccgg
    22921 gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg
    22981 gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca ggcaaaattg
    23041 aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc aggaaataaa
    23101 ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg
    23161 ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg agaacggcgc
    23221 ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat
    23281 gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc cgatgcttcc
    23341 ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg
    23401 ggcatttgat tatctgccca aaccgtttga tatcgacgaa gccgtggcgc tggttgagcg
    23461 cgccatcagt cattaccagg aacagcagca gccgcgtaat gttcagctta acggcccaac
    23521 gaccgatatc atcggtgaag cgccagccat gcaggacgtg ttccggatta tcggtcggct
    23581 ttcgcgttct tctattagcg tgctgattaa cggcgagtcc ggcaccggta aagaactggt
    23641 cgctcatgcc cttcatcgcc acagtccgcg agccaaagcg ccatttatcg cgctgaatat
    23701 ggcggctatc ccgaaggatt tgatcgaatc cgaactgttt ggtcacgaga aaggcgcatt
    23761 taccggcgcg aataccattc gtcaggggcg ttttgaacaa gctgatggcg gtacattatt
    23821 tctcgatgaa attggcgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc
    23881 agatggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat
    23941 cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggcaagttcc gtgaggatct
    24001 gttccaccgc ctgaacgtca tccgcgttca tctgccgcca ctgcgcgaac gtcgggaaga
    24061 tattccccgt ctggcacgcc attttttaca ggttgccgcg cgagagctgg gcgtagaagc
    24121 gaagttgctg catccggaaa ccgaagccgc gctgacgcgt ctggcgtggc caggcaacgt
    24181 gcgccagctg gaaaacacct gtcgctggct aacggtgatg gccgccgggc aggaagtgtt
    24241 gattcaggat ttgcctggcg aactgtttga atcaaacgta ccggaaagca cttcacacat
    24301 gcagccggac agttgggcaa cgctgttagc acagtgggca gacagagcgc tgcgttccgg
    24361 tcatcaaaac ctgctttccg aagcacagcc agagctggaa cggacgttac tgaccaccgc
    24421 gctgcgacat acgcaggggc ataaacagga agcggcgcgg ctacttggct ggggtcgcaa
    24481 caccctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt gtgtgtaaag
    24541 attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat aagttcagta
    24601 tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta atctggtatc
    24661 gagtggcgca gttgacagcc acacaccgca aactgctgtt gctgccgtgc tgtgtgccgc
    24721 gatgataggg ctgtttagct gaagctcgtt gtttacgccg gatgcggaga aagtcgtagg
    24781 cctgataaga cgcaaccagc gtcgcatcag gcgttgattg ccggatgcgg cgtaaacgcc
    24841 ttatccggcc tacaaaagca ataatttcaa cagattttgc aggcctgata agcgtagcgc
    24901 atcaggcaat cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa
    24961 gcgccattca caatttaaat cacccgagag aactgctgca tccgcgcttt ctggcgcaga
    25021 taggtatcaa agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt
    25081 attcccttct catccacatc caccagccca tcttttgcta acggggcgag caacttgaga
    25141 tcttccgcaa agtaatcagc gaagagcaaa tcccactgtt gctcaatagg ggcgtaatcc
    25201 agacggaagt tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt
    25261 agcgcaatac cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc
    25321 aactctttct ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc
    25381 cccagcagat cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct
    25441 tcacgctggg ccaccgccag ctcgtcatcc ggacgggcaa agtgatccat accgataaac
    25501 tgatagcccg attgtgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc
    25561 ggactcggca ggtcagcgtc tttgattttg cgctgagcag caaaaatggt cggcaaatgc
    25621 gcgtagttaa agacgctcag acgatcgggg ttcagctccg ccacgcgttt cagggtaaag
    25681 gcgaaactct ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta
    25741 aagccaatct cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg
    25801 ttaaccagac gttgcacttc tttgttgaag tcctgcacgc ccatgctcag ccgattaaag
    25861 ccttcggcgc gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc
    25921 gagatctccg catcggcatt gaactggaag ttttcgcgca gcagcttcat cagacggctg
    25981 atttgcgctt tgttcagata cgtcggcgta ccaccgcccc agtgcagctg gctcaccttg
    26041 cgcccggcaa aaagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga
    26101 tcggccttgt gctgctggcg agtaactatc ttattgcaac cgcagaagta acaaagctta
    26161 tggcagaacg gaatatgtac gtagagagat aatggacgct caggatagcg cgccacggct
    26221 tgtaaaaacg cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg
    26281 tatcgtggcc cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt
    26341 acagacatgc tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc
    26401 cgtttcgggt catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt
    26461 taacgaataa ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc
    26521 ccacggcggc aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt
    26581 cttcttcctc ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa
    26641 tacgatccag tttagcatcc acccaggatt gctcttcggc gctcagggtt tcgcctgctt
    26701 ccagacgttc cagcagcgca tccagacgct catccgtttc cagtaactcc aactccgcct
    26761 gcggtgaaag cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca
    26821 cgcccaatgg aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctgacctt
    26881 tgctgcctga cgtggtgtta ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt
    26941 ttttctgacg cttacggtca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg
    27001 cttttgcatg acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt
    27061 tagccgtttt attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa
    27121 aggcgacaga ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa
    27181 tccttgcagg ccgccaacac accctgtttt tccattagcc ggaacaatcc gtgttgattc
    27241 catttccatt taattacgtc tccggacgtt tatcttcctg acaatcctct gtcttcgcct
    27301 cctggcgctc ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg
    27361 tgctacttta cgcctttgct ttaaataaac aatcaaccgc catcagtatt tgcacatttt
    27421 cagagtttta tataaaagca attcactgat ttatatagct tatttgacat gcgtcgcaga
    27481 attttctctg ctatttcgca taaagactat cggcaatgtc tcacaaagca tatggctttt
    27541 acttacaacg ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccgca
    27601 aagctttgac tgatggagac gaccgctttg actaatttga attatcaaca gacgcatttt
    27661 gtgatgagtg cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt
    27721 gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc
    27781 ctggctcgta cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct
    27841 gacggcaagc gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg
    27901 aagcgcaaat ggcagcgcgc gctcggcgag tacctcgaaa aacgtcagag cctgcaaggt
    27961 ctggtggtgc taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag
    28021 tgggcggtag acagcaatat cgccgtgctg gtactgctga ccaaagcgga caaactggca
    28081 agcggcgcac gtaaagcgca attgaatatg gtgcgtgaag cggtgctggc gtttaacggt
    28141 gatgtgcagg ttgaaacgtt ttcttcgctg aagaaacaag gcgtggacaa gctgcggcag
    28201 aaactggata cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa
    28261 ttttcttgcc ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt
    28321 acaattctac cgttggcctg ataaacatgg cgtatcaggc attacggatc ttttctttcg
    28381 cccaataaaa aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc
    28441 gattacgtga agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact
    28501 aactttactc ttttttgttc agttcagaaa gcattttttg taattttttt tcagttgttg
    28561 cataggaatt tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa
    28621 aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc
    28681 caccagtaac ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc
    28741 gacggcatcg acatcatctt tatggacttc aaataccagt tcatcgtgta cctgcatgat
    28801 catacgcaca cgcggttgct cagcctgtag ccacgcatca acggcaatca tcgcccgttt
    28861 gataatgtcg gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc
    28921 acgacgagca ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt
    28981 ttcaacgtag ccctgctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc
    29041 agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct tctttacgcg gaatgttcaa
    29101 ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgcttttgc
    29161 actacgacgt tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt
    29221 tgcacggtgg atatcttttc cttccgcgaa tgcggtcagc aagcctttgt cacgcgagag
    29281 atgcgccata atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc
    29341 tggcgcaata aacgcctggc ggatacggcg accttcttcg ttacgcaccg gaatgttttg
    29401 caggttagga tcggttgacg ataaacgtcc cgttgcggtt actgcctggt gataagaggt
    29461 atgtacacgc ccggttttcg ggttgatcat caacggcagc ttgtcggtgt aggtcgattt
    29521 cagcttcgcc agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag
    29581 ttcttccagt acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt
    29641 aatgccctgt ttttcaaaga gaatggtttg taactgcttg gtggaagaaa ggttaaattc
    29701 ctcacctgca atttcatgcg ctttcttttc cagctcagcc agacgaaggg tgagctcttc
    29761 agaatgattg tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag
    29821 caccggcacc agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg
    29881 cagatccggc cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta
    29941 acgtccggcc tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttacccgc
    30001 aatatcttca aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc
    30061 gtgacgcccg gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc
    30121 acgcagctca atgccgtaat tcgccagaat accgcggtcg tatttcaggt tttgcccgac
    30181 cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgcgaga
    30241 gatttgatcg ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg
    30301 ctcgatagca aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc
    30361 ggtatcaaat gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc
    30421 ttcatcaagg atggtgacgt agttgtcata agaaatcacc gttgccgtca cttctggcgc
    30481 ttcgtctgca acactggttt cctgcggtct ggctgcgggt ttcgcccctt tagcctgtaa
    30541 ccatttgccc gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc
    30601 caacaactct tccgccgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc
    30661 aacgtcggtt ttaatcgtcg ccagctggta tgagagataa gcaacttctt tgttttgctc
    30721 gagcttcgct gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc
    30781 ggcatacagc gtatccagtc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc
    30841 gacgcccggt acgccaggga tgttatcgga ggagtcaccc atcagcgcca ggaaatcgat
    30901 gatcagttct ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt
    30961 cgtcatggtg ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc
    31021 agtgctgatc agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac
    31081 gtcgtccgct tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc
    31141 gtgcagaggt tcgatttgtg ctcgcagatc gtccggcatt ggcggccgat gtgatttgta
    31201 atgttcaaac agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg
    31261 cgtcggttta tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc
    31321 ggtaggctcg cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata
    31381 agatgaacca tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc
    31441 tgtttatcag attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc
    31501 gcttttcgtg aaaatctttt gtagatcttc tggatcgctc gcaaatcgtc atgtggataa
    31561 ctttgtgcat aaattattta catcgctttt aatttattat catgcttttt acagcatggt
    31621 gtttgtcttt gtttttcatg cagttaaaaa taaagccatc cctaataatt gagataatgg
    31681 gattttttct aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt
    31741 gggtcgtttt ctgctaaaat ctgcgccttg cgcttcttta gcgcactgtc tatggctaac
    31801 cttttgaata aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc
    31861 gtattgacca ttttggtcac tattttttgt tccgtcccga tcatcattgc cgggattgta
    31921 aaactattgc tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg
    31981 atgtattgct ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg
    32041 gaagttcacg ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac
    32101 cgtagttggg cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat
    32161 aaatatttcc tcaagcagca gctggcctgg gtgccttttc ttggcctggc gtgctgggcg
    32221 ctggatatgc cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga
    32281 cgtggtaaag atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcaccccacc
    32341 actattgtca atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac
    32401 tcctcttttc aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta
    32461 ctgggtaaac aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt
    32521 cagccgttct tcgatatgtt aagcggtaaa ctgacgcgaa ttgtcgtaca tgtggattta
    32581 cagcctattg ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat
    32641 tttcagcaat ggctgaactc attgtggcag gaaaaagacc ggcttctgac atcgttgatg
    32701 tcatcacagc ggcaggaaaa gtaagtttta ctcgcctctg aatgagcaga ggcgagtgag
    32761 tattttaatg aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc
    32821 cgttaaaaac aactttaccc ggtgtataac gcagttgcag cgaggcggta ttgttgtcaa
    32881 tcgtgattaa ctgcaacatc tgacctaatg tcatcatccc gctgatttgt ttatcagccc
    32941 gcttttgcgc tttttccgca tccatccctt cagataaatt aagctgtttt gcggtttctg
    33001 tggcgacatt aagcggtaac ttcatatcaa aattgagcga tttaatatct ttgtttgtgg
    33061 atgatgaaga tttggcgggg tcggcaatac tgatatccag attggcattt agttcgccga
    33121 gtgcgttctt ccatcttact ggttgtttaa tggtcggctc acttttttgt aataacggta
    33181 aatactcttt gaacaatgca gcattcactt cttgcagagc aacttcatcg tttgctaact
    33241 cagggtgtgc agcaagctgc ttctgcatcg caatgttata ctggatgata aattggcgca
    33301 ccgctgaggg atcaatagac tcagcaatca atgaccactc accactaccg agttgctgat
    33361 tcccttttgt taacttatca agttcgtagg tcagattgac attgatataa tctttgtctt
    33421 taacgcgatc cagcctggtt tttgcaacga aagcatcgat ttgggcaacg acttccccct
    33481 ggttgataat attaacctga ttaaatttcg actcgctttc accaaccgga aatttctttt
    33541 cttctaatcg tgtcagagaa tcaatggtaa aacttttcgc cgttactttg ttcattggtg
    33601 aaaattgcag aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactat
    33661 catcaccatt aaaagtaaac tggccttcac caaaagaaaa ctttccatac tctgcagggg
    33721 caacatctaa tgtcgtggag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg
    33781 gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag ctcattattc actaagttca
    33841 cttttgccgc tgccagtgct gggatgacat taccatgcat taacattgtg atgggcaacg
    33901 gaccgtgctc aacatccaca tcaaatacaa ttttctggcc tggcttgata ttgagatcgg
    33961 gtgcaccgtt atcaaatgtc atttgcattt gaaaacggga attaaaaaag cctttgtcaa
    34021 aatttttata acttaacgtc atatcgtagg catgttcacc tttcttttta gcatcgttaa
    34081 aacctttaat aaacttttcg acaccaggct gaatttgcgt acctgtgtac catgtgccac
    34141 caccccagat tacggctaac gcaacaatgg cacctgtagc tgacttacgt atcatataaa
    34201 tattccctga taaaacagca aaactttgaa tatccactta tgctgaaata
//
LOCUS       DADTFG010000037        33456 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.037, whole genome shotgun sequence.
ACCESSION   DADTFG010000037 DADTFG010000000
VERSION     DADTFG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.037"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            80..637
                     /locus_tag="J1138_001643"
     CDS             80..637
                     /locus_tag="J1138_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA8453452.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDAIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QFGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            679..2187
                     /locus_tag="J1138_001644"
     CDS             679..2187
                     /locus_tag="J1138_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA8453453.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGVPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            2238..4235
                     /gene="tkt"
                     /locus_tag="J1138_001645"
     CDS             2238..4235
                     /gene="tkt"
                     /locus_tag="J1138_001645"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA8453454.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYIQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAAWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(4265..5110)
                     /locus_tag="J1138_001646"
     CDS             complement(4265..5110)
                     /locus_tag="J1138_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA8453455.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLK
                     KQAEDLIQSTQFFNAEVLESILKLLANCDTIFFFAAGNSNPLAVYGAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(5310..6215)
                     /locus_tag="J1138_001647"
     CDS             complement(5310..6215)
                     /locus_tag="J1138_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8453456.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKCNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMHEHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLMTQFVFN"
     gene            complement(6293..7501)
                     /locus_tag="J1138_001648"
     CDS             complement(6293..7501)
                     /locus_tag="J1138_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBA8453457.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGTVPVIALVLCYI
                     LYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALIAAGVT
                     FLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNMFQGTL
                     VVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYIADFFL
                     YRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILVACIAY
                     AILSRASQHR"
     gene            complement(7527..7916)
                     /locus_tag="J1138_001649"
     CDS             complement(7527..7916)
                     /locus_tag="J1138_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HBA8453458.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGGWRKYTGHFHTV"
     gene            complement(7933..8889)
                     /gene="arcC"
                     /locus_tag="J1138_001650"
     CDS             complement(7933..8889)
                     /gene="arcC"
                     /locus_tag="J1138_001650"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA8453459.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRISPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(8882..10306)
                     /locus_tag="J1138_001651"
     CDS             complement(8882..10306)
                     /locus_tag="J1138_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA8453460.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELTPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(10303..11862)
                     /gene="fdrA"
                     /locus_tag="J1138_001652"
     CDS             complement(10303..11862)
                     /gene="fdrA"
                     /locus_tag="J1138_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA8453461.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALALAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNDQPLPAVETEISCADLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     SLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTAPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(11957..12817)
                     /locus_tag="J1138_001653"
     CDS             complement(11957..12817)
                     /locus_tag="J1138_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA8453462.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNTTLWQS
                     PFVGRMLTFEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGYFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(12823..13491)
                     /locus_tag="J1138_001654"
     CDS             complement(12823..13491)
                     /locus_tag="J1138_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBA8453463.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(13748..14557)
                     /locus_tag="J1138_001655"
     CDS             complement(13748..14557)
                     /locus_tag="J1138_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBA8453464.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            14819..15580
                     /gene="udp"
                     /locus_tag="J1138_001656"
     CDS             14819..15580
                     /gene="udp"
                     /locus_tag="J1138_001656"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBA8453465.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            15721..17148
                     /gene="rmuC"
                     /locus_tag="J1138_001657"
     CDS             15721..17148
                     /gene="rmuC"
                     /locus_tag="J1138_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBA8453466.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17243..17998
                     /gene="ubiE"
                     /locus_tag="J1138_001658"
     CDS             17243..17998
                     /gene="ubiE"
                     /locus_tag="J1138_001658"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBA8453467.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18012..18617
                     /gene="ubiJ"
                     /locus_tag="J1138_001659"
     CDS             18012..18617
                     /gene="ubiJ"
                     /locus_tag="J1138_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBA8453468.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18614..20254
                     /gene="ubiB"
                     /locus_tag="J1138_001660"
     CDS             18614..20254
                     /gene="ubiB"
                     /locus_tag="J1138_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBA8453469.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20333..20602
                     /gene="tatA"
                     /locus_tag="J1138_001661"
     CDS             20333..20602
                     /gene="tatA"
                     /locus_tag="J1138_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBA8453470.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20606..21121
                     /gene="tatB"
                     /locus_tag="J1138_001662"
     CDS             20606..21121
                     /gene="tatB"
                     /locus_tag="J1138_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBA8453471.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21124..21900
                     /gene="tatC"
                     /locus_tag="J1138_001663"
     CDS             21124..21900
                     /gene="tatC"
                     /locus_tag="J1138_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBA8453472.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            21942..22724
                     /gene="tatD"
                     /locus_tag="J1138_001664"
     CDS             21942..22724
                     /gene="tatD"
                     /locus_tag="J1138_001664"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBA8453473.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQELAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22721..23209)
                     /gene="rfaH"
                     /locus_tag="J1138_001665"
     CDS             complement(22721..23209)
                     /gene="rfaH"
                     /locus_tag="J1138_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBA8453474.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23376..24869
                     /gene="ubiD"
                     /locus_tag="J1138_001666"
     CDS             23376..24869
                     /gene="ubiD"
                     /locus_tag="J1138_001666"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBA8453475.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            24915..25616
                     /gene="fre"
                     /locus_tag="J1138_001667"
     CDS             24915..25616
                     /gene="fre"
                     /locus_tag="J1138_001667"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBA8453476.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(25889..27052)
                     /gene="fadA"
                     /locus_tag="J1138_001668"
     CDS             complement(25889..27052)
                     /gene="fadA"
                     /locus_tag="J1138_001668"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBA8453477.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27062..29251)
                     /gene="fadB"
                     /locus_tag="J1138_001669"
     CDS             complement(27062..29251)
                     /gene="fadB"
                     /locus_tag="J1138_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBA8453478.1"
                     /translation="MLYKGDTLYLDWLEDGITELVFDTPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSHAKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRDKARHNEPYYPPVEPARPVGDLKTA"
     gene            29441..30772
                     /gene="pepQ"
                     /locus_tag="J1138_001670"
     CDS             29441..30772
                     /gene="pepQ"
                     /locus_tag="J1138_001670"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBA8453479.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30772..31386
                     /locus_tag="J1138_001671"
     CDS             30772..31386
                     /locus_tag="J1138_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBA8453480.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31425..32876
                     /gene="trkH"
                     /locus_tag="J1138_001672"
     CDS             31425..32876
                     /gene="trkH"
                     /locus_tag="J1138_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBA8453481.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            32888..33433
                     /gene="hemG"
                     /locus_tag="J1138_001673"
     CDS             32888..33433
                     /gene="hemG"
                     /locus_tag="J1138_001673"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBA8453482.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7870 a         8363 c         8983 g         8240 t
ORIGIN      
        1 atttcatact gatgcattgg aatgaaataa aatttcatga gattttgtgt ttgagaaaaa
       61 ttatttcatg aggtgtgaaa tggacgcaaa gctgtacatc actgagatca tcaatgaatt
      121 aaccttcgtt aaggactcta tccgtgagga tgactgcacc aaattgatcg acgctattca
      181 gcaaagccag cgcgttttct gctatggcct ggggcgtgcc ggcttttcca tgaaagcctt
      241 caccatgcgc ctgatgcata tgggcaaaga ggtttacttc ctgacggaaa ccattacgcc
      301 gaactttggg cctggcgatc tgtttatcgt ttcttccgct tccggtgaga cagcacaact
      361 ggtggcactg gcgaaaaaag cacgtcagtt tggcggcgct gtggcggtgt taaccacgaa
      421 tcgacacgcc actatcaccg agtttgtcga cgtcatcgtg caaatcaatg ccccttccaa
      481 aaatcaaaaa gattctgttt tccgctcggc acagccgatg gcgtcgttgt atgagcaagc
      541 tttgctggtg attgccgatg cattagtgat gaagatggct gctgaaagcg gtgcgccgga
      601 aagtgaactg tttaagcgcc acgccaatct ggaatagtcg tcgccgttat ttgctcgata
      661 ccctacagga gaataaaaat gagcaaaaaa ataacatcct tgtttaactt caagaagttg
      721 cagaaatttt cttctgctct gatgttacca atcgccattc ttccggcggc gggtttgatt
      781 ttagccatcg ggatttcatt taacattgag ctgctggcga attgcggcgg cctgattttc
      841 gccaaccttc ctttgctgtt ttgtgtcggt attgtgattg gcctgacagg tgatggttct
      901 tcatcacttt cagcgattat cagttacatg atcatgaatg ccacgatggg aacgcaactt
      961 ggcattaccg cggagtccgt tgcagccagt aacggtgact atgcgatgat cctcggggta
     1021 ccgacgttac aaacgggagt gtttggcggt ttgattgtcg ccatctttgt ttatctgctc
     1081 tatacccgct tccacaatat tgaattgcct cagtttcttg gtttcttcgg tggaaaacgc
     1141 tttgtaccga ttattacggc ggtggcggca attattgtcg gctatttatt gccgtggctg
     1201 tggataccgg tacaaaaagg tctggcgtcg ctttcaacgc tggtgaccag tgggcataac
     1261 gcgaacttcg cggcctttat ttacgccgtt ggcgaacgtt cgctgattcc ttttggcctg
     1321 catcatatct ggaacgtgcc gttttattac aactttggcg actacatgac caaatccggg
     1381 caactggtga cgggcgatat tccggtgttc tttgcccaat tgcgcgacgg tgttccgttg
     1441 acggcggggt tgtttatgac aggtcgtttc ccaatcatga tgtttgcgtt acctgccgcc
     1501 gcgttggcga tgtatcagga agcaaaaccg gaacgtaagg cgctgattaa aggcctttta
     1561 ctctccggtg cgctgaccac atttgttacc gggattacgg agccgctgga atatgcattt
     1621 ttgtttgcag ctccggttct ctacatcatt cacatcttct tatatgccac ttcatttgtg
     1681 ctgatgaaca tcctgaatgt gcacattggt catccttttg ctggcggtct tattgatttc
     1741 gtactgaatg gcgttatgcc aaaccgcacg ccgtggtatc tggtgttcct ggcaggcgcg
     1801 ggctatgcag cgatttacta caccgtattt cgggtactga tccgcaagtt gaatctgaaa
     1861 acaccgggac gtgaggatga agagattgcc gttgcaacca cattaacgcg cgaagaacgt
     1921 ccataccaaa tcatcgaggc agtaggtggt ttcgacaata tcgaagatgt ggatgcctgc
     1981 gcaacacgcc tgaggctggc gctggtagac gataagaaag tgaatgaaaa acggctgaag
     2041 gagttggggg ctgcgggttt agtgaaactg ggtgatggcg gtgtacaggt gatatttggc
     2101 ggcaaatcgc aaatcctgcg cgacgaaatc aaaaccgtaa tgagcagacc gcgcccgaca
     2161 gaaatgacag tggcctgtgc taactaattc gagattagcg ggctggtttc agcccgccag
     2221 acagagagag ctgaagtatg aattcacaat tactggctaa cgccatccga atgttaagtg
     2281 ttgatgcgat ccagaaagcg aactccggtc atccgggcgc gccgatgggg atggcggata
     2341 ttgccgaagt ggtctggcgt cgtcacctgc gtcataaccc aaaaaatccg cagtggttta
     2401 accgcgaccg ctatattcag tcgaacgggc acggttccat gttgatttac gctttgctgc
     2461 atcttacagg ctacgacctg tcgatggatg atattcgcga ctttcgtcag ctccactccc
     2521 gcacgccagg tcatccagaa tatggttata cgccgggtgt tgaaaccacc accggcccgt
     2581 tggggcaggg cgtagctaat gcggtgggga tggcgattgc ggagaaggcg ctggcggcag
     2641 aatttaacaa accgggcttt aatattgttg atcatcacac atggctattt ttgggtgatg
     2701 gctgcctgat ggaagggatc tcccacgagg cctgtggtct tgcaggaacg ctgaagctcg
     2761 gcaatctgat tgctatctgg gacgataacg gtatttcgat cgatggtcac gtagaaggct
     2821 ggttcgctga agatacggct gcccgttttc gtgcctacgg ctggcatgtc attgaaggcg
     2881 ttgatggtca cgatccagaa gcggtagatg ctgcggtgcg tgaagcgaaa agcgtcacgg
     2941 ataaacccag cttactgtgc tgtaaaacga taattggttt tggctcaccc aataaagcca
     3001 acagccacga ttgccacggc tcggcgctgg gtgcagatga agtggcgctg gtgcgtgaac
     3061 gtctgcaatg gccgtatgcc cctttcgaaa ttcctggtga aatttatgcc gcatgggatg
     3121 cgaccgaaaa aggcgcacag gttcaacagg aatgggatgc actgtttgcc gactacgcga
     3181 agcagtggcc ggaactggcg gcggagttta ctcgtcgcat gaagggtgat ttgcctgctg
     3241 gctgggtcga gaatatgcag aaatatgtgc acgatctgca atcccatccg gcggcgctgg
     3301 caacccgtca ggttagccag aaatgcctca atcacttcgc agatatgctg ccggaactga
     3361 tgggcggctc ggcggatttg tcgccttcta acctgactcg ccatcagaaa tcggttgatt
     3421 tcaccgggga aaatccggcg gggaattaca tctcctatgg cgtgcgtgaa tttggtatgt
     3481 cggccatcat gaacggtctt gcgctgcatg gtgggtttat cccttacggc ggtactttcc
     3541 tgatgtttat ggaatatgcg cgtaatgcac tgcgcatggc ggcgctgatg aagatccgct
     3601 cggtgtttgt ttatacccac gataccatcg gcctgggcga agacggcccg acacatcagc
     3661 ccgttgagca actggcgtcg ctgcgtctga cgccgaatat ggaaacctgg cgcggttgcg
     3721 atcaggtgga agtggcggtg gcctggcaac aggcgattga gcgcaaagat ggcccaacgt
     3781 cgttggtatt aacgcgccaa cctctggcgc aacagccgcg caccgctgca cagttggcag
     3841 agattgcgcg tggtggttat gtactgagtg attgtgacgg tcagcctgag atgatcctca
     3901 tttctgcggg ttcagagatt gagttagtgg tcagcgccgc aaaagcgtta acggaagaag
     3961 ggcgtaaagt tcgcgtggtt tctatgccgt gtaccgagcg tttcgataat caggatgcgg
     4021 cgtacaaaga atcggtattg ccaaaagctg tacgcaaacg tctggcggta gaagccagca
     4081 tcgccggatt ctgggaacgc tacgtgggcc tggacggtaa agtgatcggc atgacgagct
     4141 ttggtgagtc tgctccggcg aatgtgttgt tcaaacactt tggttttacg ccggaaaatg
     4201 tcctggcgca ggcgcgtgag ctactgaata gctaaacctg cacaaggggt gaaaggatga
     4261 ccttttaccc cttgaaacgg gagccgagaa actcttcacg ggtgcggtat ttctcgtaat
     4321 gcgccttgtt tttatacata aacaacagca ccagcatatc aataacgccc attgccggaa
     4381 cgcgagtgga gtcgaaggct tcaaagaaga ttttatcgct gaccgcggta ttgagttgat
     4441 gggtggagag ttttgataac ggagatttaa tatagttagt gatacaaacg gatttggcac
     4501 cgcgctcttt caccacttta aagatatcga ccattaagtt ggtgctgcca gagttagaaa
     4561 taccaatcgc caggtcgcgt ttccccatag aatatgccgc attaatttgc atttccgggc
     4621 tgacgtgaac aacggttttt aatccgagtt ggctgaattt ataagcacca taaacggcca
     4681 gagggtttga atttccagca gcaaaaaaga aaatagtatc gcaattagca agtagtttga
     4741 gaatggactc aagcacttcg gcattaaaaa attgcgttga ttgtattaaa tcttcggcct
     4801 gtttttttaa taagaaggtt gccaaagatg ttatatcgct gaagtcggta ttttcaggat
     4861 agctattgtc aggattaatg acttcttttg ctaatgcaat ttttaaatgg taataaccct
     4921 gataacctaa tttcttacag gtgcgtacga tagtcgcttc actggcatgg cttttttctg
     4981 atagttcagt gacactatag ttcacgactt catgtgcgtg gctaagaata tagtcagcga
     5041 cctttttttc cgcctgatgc agtgtagggt aattgctttg aatcagactt tttacggtta
     5101 tgttattcat aattagtaaa tttcattctg acagaaagtg ctgattataa catagttttc
     5161 aggcgtggat aggtgtgaaa aaatgaaggt gtgcagtgaa cgtaatgatt aacagaccgg
     5221 ttaattttca tgaattacgt caactgcaca aaaataattt aaacgggcgc tctaattaaa
     5281 gcaagactta tatttttatc attgtattgt caattaaata caaattgtgt cattaattgt
     5341 gggtttctta aatattctat gcaccactga caggctttgc ccataccatc ggaacgagcg
     5401 gcaagaaata acttggtggc gtgtttatgt tcgtgcattg gctttttaag caatttaccg
     5461 ctgttcaata acggcaatgc cagatgatgt ggaatataac caataccgac gttttgttct
     5521 attaaggcga ttgccgtagc gaagtcagga acaaaaatgg gtttttgtcc ttccaacaac
     5581 caggcttgca tcggggggaa gttaacggag gtatcgcgga tacaaaccgc tggataatga
     5641 cgcaattcac tgttttctaa tggatgccgc ttcgctgcta gtgggtggag tgggctgacg
     5701 acaaaatccc attccatttg tccgaccggt tcgctaatga tgccttcact gcttggcact
     5761 gcatgaggag caccgtagac cagatcggca cgcttactgt acagcgcatc ccagcagccg
     5821 ttgtatacct caagatctac cgtcagtgtt gttgatgtga attgccgttc aaaatctcga
     5881 ataaatgcca ccagcaaatc accaggaata atattgttta ccgcaattgt cagttcacgc
     5941 tcaataccat cgtgaatcag cactgtgttg cactttaatg cttccaggtc gccaagaata
     6001 gttttgctat gttcgatgaa gtaacttccc gctggcgata attcaatata gcgtccctta
     6061 cgaatgaaca gttcaacgcc caggctctct tccgctttct tgatggtgta actgatggcg
     6121 gaagggactt tatttaactg ctcggctgcg gtggtaatgc tctggtgtct ggcgaccaga
     6181 tgaataacac gaagcgtttc gtctgagatg aacataaggc tccctggtca gtggtaaagt
     6241 ggctgaatct ctccagattc agccgtaatc attgcctgag tatacccagg aattagcggt
     6301 gttgacttgc ccgactgaga atcgcatacg cgatacatgc caccagaatc gagtctaccg
     6361 aagggatcgt cgtcagggtg aataagcctt ttacggtcat aaagccaacc gctgcgccta
     6421 tgatccatgc tgcgaatgtc agcactttaa tctgtgactc ctgggctaac actgactctt
     6481 gatagccatt acggcgataa aggaaaaagt cagcgatata aattccggca acgggtggcg
     6541 tggcgatacc caggaacagc agaaacggaa taaaccacgc cataatatcc atactgccta
     6601 cgatggcgga aaggataccc agcgccacgg taatctgcca tttgggaaag cgggtaagca
     6661 gtgtggaaac caccagcgtg ccctggaaca tattgcccgc gttaccggtg acgcaggcga
     6721 aaatcaacaa cagcgccgca ggcagaaccg caccgaatac cgccatcgca cccagtaaag
     6781 atccctgacc actaatggcg ctcggcgttg cacccgccca gtagagcaga gggtaggcaa
     6841 ccagaaacgt cacgcctgcg gcaatcagcg catgtttgcg gttatgcaca aaactaccga
     6901 aatcaggcag tgtggcaacc agcacaataa tggtgccaac aacggtggaa acggcgacac
     6961 ctgtattcat ctccacaatc gccgctggta ctgccacttc ggtatgggtt gccacataga
     7021 ggatgtaaca cagcaccagc gcgataaccg gcacggttcc cagaacggca aagccaaatg
     7081 ccgtagaggc gacaaaaatg acacagccag ccgccactaa cagtgccagt ggaacttcca
     7141 gtccatgttg agccagtaag tcatgtaccg aatgaccaaa catattggcg gtaacggcaa
     7201 tccagccgaa cagacttatc gccatcaaaa tgttaatggc gatggcccct ttttcgccaa
     7261 acgaatattt cacaatgcca tacgttggca ggcgggcttt ttctcccacg ctaatggtga
     7321 tggctgaaag taaggtcaga atgacgccgc ctaacaatac gacgctaatt aaactgccgc
     7381 tggaaagttg attgccgagg cttgaggatg acaacagcac aggtgtgacg gcaataccca
     7441 gcaaaatcat ggcaatacgc aatcctgatg ccgtattcag attctcttct tttttacgca
     7501 tagtatgttc cctttataaa tgcgcgttac acagtatgaa aatgcccggt gtatttgcgc
     7561 cagccgccca gttctgtaat ttcccgttgg ccttcggtca gccagttttc tgccagtacg
     7621 cccagcactt cgctaccatc ggcaagtttg attttgccaa ttgccagccc agcgggttcg
     7681 ctcattaaca gagcggcaaa actggccagc ggtaattgcc aaatttctac gtcgacgtgt
     7741 gttcctcctt cattcacgcg aatcattccc ggatggcgat cattaatact ccacaaccgg
     7801 taatgggcgt cagttcggtc ttcacggaca aaaataccgc ccgctttttg catatttggg
     7861 ttgagttcca ggccgcgcat cagcgttccg ttaacggcta aaagcgttgt agtcattgtg
     7921 ttctccagag aattaaaggg ttatccatgt accgctctga tgcgccaggg ctgcttttat
     7981 ggtttgcggt gaagtaatca ggccgctggc ctgtttgcct tgttgctggc tggcattgat
     8041 gaaatcaaca atggcttcca ctttgggttg catactgccg acaccaaact gaccttcccg
     8101 caacagcgtt tgcgcttcgg caacgctgat agcgtcgagc cactgttgtt gtggtgtacc
     8161 aaagttaatc gctacttttt ctacaccggt ggggatcacc agcagatctg cgcctaactg
     8221 actggcgagc agcgcggagg ccagatcttt atcgatcacg gcttccacac ctttcagttg
     8281 ttgcccgtct cgcaccaccg gaataccgcc gccgccgcag gcaataacca gatatccctg
     8341 gcgcaccagg tgagcgatgg tgtcgtgctc aataatttcc agtggtgcag gagagggaac
     8401 tgtacgccgc caaccacgcc cggcgtcctc catcagcgtc cagccgagtt gttgttggcg
     8461 ttgttgggcc gttgcttcat cgagaaacgc gccaattggt ttactggggc tggcgaaagc
     8521 atcgtcatgt gggctgattc gcgtttgtgt caccagggca attaccggtt tgtttatgcc
     8581 acggcgagcc aattcgttat gcagcgcttt ctggaacatg tagccaattg caccttgtgt
     8641 atcacccacg gcgtaatcaa gtggaaccgg agaaacttcg ttactggcga gttcagagcg
     8701 gcgcagaata aagcccacct gcgggccatt accgtgggtt agtaccaggt cccatccggc
     8761 ttcaaccata tcaacgatat gttgcacgct ttccatcact gcaacatatt gatcgggaat
     8821 actattgcgt tgctcgtcct gaatgagcgc attgccgcca accgcgacga cagccagtgg
     8881 cttaaccatg atggttctcc tttgcgacga tctccgccag tgcacgtaca gcggcgacaa
     8941 aacaggccat cggcgcagtg gtgatcccgg caccaatttg cccgaccccc gcttgtttgt
     9001 gggcgatacc cgtattgatg accggtaaaa tgccgcgatc ggtcacttta cgcgcatcaa
     9061 tccctgctgc ggtgggggca aaattgaggg ctggaagtgt aaatgcgggg ttaccgccga
     9121 gggtgatggc ttgcatccga cggctgttat tggtggcatc ggcaggggtg cctcccacaa
     9181 atttgacgat cgccggagac gacgccatcg cgaagccacc cacacctgcg gtttcagtaa
     9241 ttgcgctatc acccaggtca gcagcagcgt cgtcaacgcc aaatccgggg aagaacagac
     9301 cttcaacggc attggcagga gcctggaacc agcgatcgcc agtaccggaa agccgaatac
     9361 caaaattcac cccgttacgc gccatcaccg taaccattga actgaacggt acattggcgg
     9421 cggcatccat cattgcttta caggcggcca tcgacagatt gaggaagaag tgatcgttac
     9481 cggtgataaa tgccacgacg cgctggatat gttcctgtgg aagagagcag gccagcaatg
     9541 ccgggagcag gcgcttaatc agcaaaccgg tagcggcagc attgcggtta tgaacttcat
     9601 cccccatatg cagcgcctgc gccattaatg gcttgagttc cagctcgccg tgttgtgcga
     9661 ttgctgcttt cattgccggc gtcagttcat cgcgcatcca ggccagacga ttgagaactt
     9721 catcgttatt ggcgccaaaa cggagaacct tacccagacc ttcattgaaa ttgctaaaag
     9781 tacggtggcc gttggttttg ttttctacca cccataacgg catcgacggg ctgatgatgc
     9841 ctgccattgg gccaaccgcg tgataatgat gacaaggggc aaggtcgatt tcgcctgcgt
     9901 ttatctggtt ttctgcgtct tgctggctgg tggcccaacc ttcgtacagc atggcaccaa
     9961 taatcgcccc ttttaccgga ccacacatct cttcccaggc aatcggcggg ccagagtgca
    10021 aaatacgttt ttgttcagcc attgctggaa tggcctcttt agcaagcatg acatccacca
    10081 gcacgggttg tgcttcgaga taacgtgtca tggcgatttg gttggcattt tctaccaggg
    10141 gatgacgtag caacgatgcc agatccaatc cagcgtcaat atcgccttgt gctggtggct
    10201 gccagttgag tgcaattgcg cttcccccgg catgttgaat attgtcagca aaactgtgta
    10261 gtcccgcgtt cacaaccttc aaaggctgat taaataaggt attcatgcgt ttagcccctt
    10321 tttctgcaac tgagtctgtg caacggtgct ggcccatagc gccgcctgtg cattgctgct
    10381 ggcgatgatg acccctgcat tttgtagtgc gcttatctgc tgactgcgta tttgcggatc
    10441 agcttccgtt ccgcaaacgt gcgcaatcag caacggtgcg ttattcatgt cgatgtgtga
    10501 aagttggtcc agtaattcgc tggcaggcgc cgtggaagcg ccataaccaa gtaccaggtc
    10561 gaataacacg acggcagtgt gtgagtcgtt gagttcgttg agcagacgtt gattgcgcag
    10621 agtcgggtcg atcattggat gcggtttgcc acgggtgaaa tcatcgtcgc ccatatcgat
    10681 caacgagtga tcttcgcttt gccagatatt tgccagagca cgattacccg caactggcgt
    10741 attggaagct gcgctgaatc ccttttgttg gcagataagt tgcgcttcgt agcagaaggt
    10801 tcctccggcg aaaacaccgc gtatcgcctg gcgatgggct ggcaggcgct gacaggcgtt
    10861 ttgtaacatt gtgagatcgg cgcaggaaat ctcggtctcg acagccggaa gtggttgatc
    10921 attcagtaac gccacggcga tatttgcggc gctggcaagt gtggtggccg cggtgatatt
    10981 gggacgtgcc agatcatgcg gattcgcacc gaggaaattc accaccacgg gttttccaca
    11041 agcctcggcg cgctccagaa tagtccgggc aacgatcggt gaaggcggtt ttgaaatcag
    11101 cacaattacg cgagtttcat cgtcttgcgc cagcgcatct aaagcgaaga gcatcgatat
    11161 tccaccaatc tcttcactga gatcatgtcc gcctgttccc aacgcctgag aaatacccgc
    11221 tccgagctgg tcgattcggc aagtcacttc ctgtagcccg gtgcctgacg cgccaataac
    11281 accaattgct ccgcgtttca ccacattagc gaagcctaac ggaatgccgt taacaatggc
    11341 ggttccacag tcaggtccca ttacgatgcg ctgacgttct cgggcaagcg ttttgatgga
    11401 tttttcctgc ccgatactga cgttatcgct gaacatcatc acgttcattc cgaggtttaa
    11461 cgctttaatt gcttcggcgg cggcgtagtc tcccggtaca gaaatgagtg ccaggttggc
    11521 ttcgggttcg gcttccagtg ccatttccag gctgaccttt tccggtgttt taatgccact
    11581 atccgtttca tcatcgggtt tgctggtcag acgttgctgg gccagtgcca gtgcttcatt
    11641 gcaaatatct tcttcaccca tgactgcaat cactaaatca ttgggactgg cgttgatttc
    11701 gttacccaga cccgcatcgc gcaattgttc gagattattg ggcgttccca tgacgaccga
    11761 ggcctgttga ataccgggca acttgctgat ttgcgcggaa atctgcatca gagaaactga
    11821 gtcctgataa agattactga aaactttata tttaactttc atgctgttac gcctgtttca
    11881 ttccgcccga tagcaaaatt cgggcaaagc atccccgtgt agccccataa acgggctacg
    11941 ggagaaaagg ttgaaatcaa ttcatggtgt tcagggttcg aaagccatgc agcattcctg
    12001 caaggcaatc tactcctgac gttgcgccaa attgcatgac ctgttctgca caggatgcga
    12061 ttgtcattgc cgatgcgctc ccaaccagtt gtgcgagtaa ctggcaaatc ggttctgaaa
    12121 aatatccctg aagcgcacgc tccagataat gacgactgat atccgtggtg ttgtgcgcat
    12181 gttgatcaat tgcctgctgc aaccggtatt gatgatcggc aagtggtgca gggagttgcc
    12241 ataaccagag cgctgccaga tagcccaaca aatagtcatc tccatcgggc gttaagcctt
    12301 taccaaagcc gatcagttgt ggcacctggc gttccagcgc atctggatgt tccataattg
    12361 ctaatccctg gtgcgtgagg ctatccggca ggtgcaatgt gctttgaacg ttatgttcca
    12421 gacaaaataa aagccgttgc gatgccatcg ttgggtagtg ctggaaaacc aaagggaatg
    12481 cctcaaatgt cagcattctc cccacaaatg gcgactgcca gagcgttgtg ttatcgagcg
    12541 ccacacaaaa tcgaggtgtc cgcagcaagc ctggggttaa ctgaatggga tctgaatggg
    12601 caatctctct tcgccagtcc caacattcag gtagccagac tctggcagca tcagggagat
    12661 tcagatacgt ttgcgcacac aacagagtga tgaatgttcc cgcgtctgaa tacagattta
    12721 ccgcatgttt aaaagcgcta tgcagacgta aatcttgcgt ttcattctgc gggaaaagat
    12781 atcccaacga cgatgcgcaa agcgtaacct gtaccatcgg cgttagcctt ccagacctgc
    12841 aataatggct ttggaatcag taacccaacc gaaaatggca ccttgtgctt tgatcatctc
    12901 gagggcgtat ttctggaatt ccgggaaata ggagccgaca caatcttccg ggataatgca
    12961 ttcatagcca cgatcatttg cttcgcgcac ggtggtggtt acgcaaactt cggttgttac
    13021 accacaaact atcagcgtct tgataccgtg gttttgcaaa atcagatgta agtcggtttg
    13081 ataaaacgcg cctttgccag gcttatcaat caccggttcg cctgccaccg ggtacagttc
    13141 aggaatgatg tcatgacctg cttcacctct gaccagaatg cgtcccatcg gtcctggttc
    13201 accaataaag gtttttccgc cgcgcgtcag tttggctggc gggcagtcgc ttaagtctgc
    13261 acgatggcct tcacgagtat gaataaccat aattcctttt tgacgtgcgg ctgccagcac
    13321 ctctgtacat ggtgcgatag ccgtgcgtac cagtgaaaca tcattgccca gtgcctcacc
    13381 gaagccccct gcttcgacaa agtcacgctg catgtcgatc atgaccagtg cagttgtgcg
    13441 cggatcaaaa ggtaattcaa agggttgagc ctgaaaaatg gattgcgtca tggtaaatct
    13501 cctgtctgat gtttaaacga tccagttgtt ctgagaacga gtattcagga gaaaagcggc
    13561 acaaaacagg aaaaaagttt gccgcacagc aacgaaattt ttgcattcag caggtggtgt
    13621 gatgacgaat gtgaagggag tcaaagagtg gcatacagca acaaattttt tgatgctgta
    13681 tggttttgag attaaaaaaa tcgttgaaat tgtgtgagct taatcatcag atttagcgac
    13741 tatggcgtta ctttttaaca ccatactgtg taaaccattc caacattcgc tgccagccgt
    13801 ctttcgcaga ttcggcatga tagctcggac gataatcggc gttgaacgca tgccctgcat
    13861 ccgggtacac gataatctct gctttcgcgt ttgctgcccg cagcgcctgg cgcatcgttt
    13921 ccacgctctc ttgcggaatg ctgttatcct ggccgccata taagccgaga accggcgcgt
    13981 taagatcggt tgcgatatca acaggttgtt tcggtgaatt cagcgactta tcgcccgtca
    14041 gtttgccgta ccacgccact gcggctttta gctgtggatt atgcgcggca tacagccagg
    14101 tgatacgtcc accccagcag aatccggtga tcattaaacg atgaacatcg ccgccgttgc
    14161 gggaagccca actggcgaca tgatcgagat cggccagcac ctgcgagtca ggcacttttg
    14221 ctaccagacc gctaagcaac gtggggatat cggcaaaatc attcggatcg ccttcgcgga
    14281 agtaaagttc aggtgcgata gccagatacc cctccagcgc cagacggcga caaatgtcgc
    14341 ggatatgttc atgcacgcca aaaatttcct gcactacaat gaccactggc agtgggccat
    14401 cgctttgctt tggtctggca tggtaagcag gcatgttatc cccttgtgaa gggatagagg
    14461 tgaagcccgc cacaatcgcg tcgtccggag tctgaacgat ggtcgaagcg agaggcgatg
    14521 cagcaggtgc aaatccagat tgttgtgttg ttgccatggt attctccgta cccttataaa
    14581 aatgttgcgc aatgttaact atagtcagca tgcaacaaat cacattgcct gaatcggctc
    14641 atcttttatg cagtcctgca gaatgaagag tgatttatgt gatttgcatc acttttgatg
    14701 ggtaaattta tgcaacacat tttcgtcatg gtgatgagta tcacgaaaaa atgttaaacc
    14761 cttcggtaaa gtgtcttttt gcttcttctg actaaaccga ttcacagagg agttgtatat
    14821 gtccaagtct gatgtttttc atctcggcct cactaaaaac gatttacaag gggctacgct
    14881 tgccatcgtc cctggcgacc cggatcgtgt ggaaaagatc gccgcgctga tggataagcc
    14941 ggttaagctg gcatctcacc gcgaattcac cacctggcgt gcagagctgg atggtaaacc
    15001 tgttatcgtc tgctctaccg gtatcggcgg cccgtctacc tctattgctg ttgaagagct
    15061 ggcacagctg ggcattcgca ccttcctgcg tatcggtaca acgggcgcta ttcagccgca
    15121 tattaatgtg ggtgatgtcc tggttaccac ggcgtctgtt cgtctggatg gcgcgagcct
    15181 gcacttcgca ccgctggaat tcccggctgt cgctgatttc gaatgtacga ctgcgctggt
    15241 tgaagctgcg aaatccattg gcgcgacgac tcacgttggc gtgacagctt cttctgatac
    15301 cttctaccca ggtcaggaac gttacgatac ttactctggt cgcgtagttc gtcactttaa
    15361 aggttctatg gaagagtggc aggcgatggg cgtaatgaac tatgaaatgg aatctgcaac
    15421 cctgctgacc atgtgtgcaa gtcagggcct gcgtgccggt atggtagcgg gggttatcgt
    15481 taaccgcacc cagcaagaga tcccgaatgc tgagacgatg aaacaaaccg aaagccatgc
    15541 ggtgaaaatc gtggtagaag cggcgcgtcg tctgctgtaa ttatcttctc ttgtctgaag
    15601 gccgacgcgt tcggcctttt gtatttttgc gtagcgcctc gcaggaaatg cctttccaac
    15661 tggacgtttg tacagcacaa ttctattttg tgcgggtaag ttgttgcgtc aggaggcgtt
    15721 gtggatttct caatcatggt ttacgcagtt attgcattgg tgggtgtggc aattggctgg
    15781 ctgtttgcca gttatcaaca tgcgcagcaa aaagccgagc aattagctga acgtgaagag
    15841 atggtcgcgg agttaagcgc ggcaaaacaa caaattactc aaagcgagca ctggcgcgct
    15901 gagtgcgagt tactcaataa cgaagtgcgc agcctgcaaa gtattaacac ctctctggag
    15961 gccgatctgc gtgaagtgac cacgcggatg gaagctgcac agcaacatgc tgacgataaa
    16021 attcgccaga tgattaacag cgagcagcgc ctcagtgagc agtttgaaaa cctcgccaac
    16081 cgtatttttg agcacagcaa tcgccgggtt gatgagcaaa accgtcagag tctgaacagc
    16141 ctgttgtcgc cgctacgtga acaactggac ggtttccgcc gtcaggttca ggacagcttc
    16201 ggtaaagaag cacaggaacg tcataccctg acccacgaaa ttcgcaatct ccagcagctc
    16261 aacgcgcaaa tggctcagga ggcgatcaac ctgacgcgcg cgctgaaagg cgacaataaa
    16321 acccagggca actggggcga ggtggtattg acgcgggtgc tggaggcttc cggtctgcgt
    16381 gaagggtatg aatatgaaac tcaggtcagc atcgaaaatg acgcccgctc gcggatgcag
    16441 ccggatgtca tcgtgcggtt gccgcaggga aaagatgtgg tgatcgacgc caaaatgacg
    16501 ctggtcgcct atgaacgcta tttcaacgcc gaagatgact acacccgcga aagcgcgcta
    16561 caggaacata tcgcgtcggt gcgtaaccat atccgtttgc tgggacgcaa agattatcaa
    16621 cagctgccgg ggctaagaac cctggattac gtgctgatgt ttattcccgt tgaacccgct
    16681 tttttactgg cgcttgaccg ccagccggag ctgatcaccg aagcgttgaa aaacaatatc
    16741 atgctggtta gcccgactac gctgctggtg gcgctgcgca ctatcgccaa cctgtggcgt
    16801 tatgagcatc aaagccgcaa cgcccagcaa atcgccgatc gtgccagcaa gctgtacgac
    16861 aagatgcgtt tgttcgtcga tgacatgtca gcgattggtc aaagtctcga caaagcgcag
    16921 gataactacc gccaggcaat gaaaaaactc tcttcaggtc gcggaaatgt gctggcgcag
    16981 gcagaagcgt ttcgcggttt aggggtagaa attaaacgcg agattaatcc tgatttggct
    17041 gaacaggcgg tgagccagga tgaagagtat cgactgcggt cggttccgga gcagccgaat
    17101 gatgaagctt atcaacgcga tgatgaatat aatcagcagt cgcgctagcc cattgggggt
    17161 agttaagccg ggtagaaatc tagggcatcg acgcccaatc tgttacactt ctggaacaat
    17221 tttttgatga gcaggcattg agatggtgga taagtcacaa gaaacgacgc actttggttt
    17281 tcagaccgtc gcgaaggaac aaaaagcgga tatggtcgcc cacgttttcc attccgtggc
    17341 atcaaaatac gatgtcatga atgatttgat gtcattcggt attcatcgtt tgtggaagcg
    17401 attcacgatt gattgcagcg gcgtacgccg tgggcagacc gtgctggatc tggctggtgg
    17461 caccggcgac ctgacagcga aattctctcg cctggtcgga gaaactggca aagtggtcct
    17521 tgctgatatc aatgaatcca tgctcaaaat gggccgcgag aagctgcgta atatcggtgt
    17581 gattggcaac gttgagtatg ttcaggcgaa cgctgaggcg ctgccgttcc cggataacac
    17641 ctttgattgc atcaccattt cgtttggtct gcgtaacgtc accgacaaag ataaagcact
    17701 gcgttcaatg tatcgcgtgc tgaaacccgg cggccgcctg ctggtgcttg agttctcgaa
    17761 gccaattatc gagccgctga gcaaagccta tgatgcatac tccttccatg tgctgccgcg
    17821 tattggctca ctggtcgcga acgacgccga cagctaccgt tatctggcag aatccatccg
    17881 tatgcatccc gatcaggata ccctgaaaac catgatgcag gatgccggat tcgaaagtgt
    17941 cgactactac aatctgacgg caggggttgt ggcgctgcat cgtggttata agttctgaca
    18001 ggagaccgga aatgcctttt aaacctttag tgacggcagg aattgaaagt ctgctcaaca
    18061 ccttcctgta tcgctcaccc gcgctgaaaa cggcccgctc gcgtctgttg ggcaaagtgc
    18121 tgcgcgtgga ggtaaaaggc ttttcgacgt cattgattct ggtgttcagc gaacgccagg
    18181 tcgatgtact gggcgaatgg gcaggcgatg ctgactgcac cgttatcgct tacgccagtg
    18241 tgttgccgaa acttcgcgat cgccagcagc ttgccgcact gattcgcagt ggtgagctgg
    18301 aagtgcaggg tgatattcag gtggtgcaaa acttcgttgc gctggcagat ctggcagagt
    18361 tcgaccctgc ggaactgctg gccccttata ccggtgatat cgccgctgaa ggtatcagca
    18421 aagccctgcg cggaggcgca aagttcctgc atcacggcat taagcgccag caacgttatg
    18481 tggcggaagc cattactgaa gagtggcgta tggcacccgg tccgcttgaa gtggcctggt
    18541 ttgcggaaga gacggctgcc gtcgagcgtg ctgttgatgc cctgaccaaa cggctggaaa
    18601 aactggaggc taaatgacgc caggtgaagt acggcgccta tatttcatca ttcgcacttt
    18661 tttaagctac ggacttgatg aactgatccc caaaatgcgt atcaccctgc cgctacggct
    18721 atggcgatac tcattattct ggatgccaaa tcggcataaa gacaaacctt taggtgagcg
    18781 actacgactg gccctgcaag aactgggacc ggtatggatc aagttcgggc agatgttatc
    18841 aacccgccgc gatctttttc cgccgcatat tgccgatcag ctggcgttat tgcaggacaa
    18901 agtcgctccg tttgatggca agctggcgaa gcagcagatt gaagctgcaa tgggcggctt
    18961 gccggtagaa gcgtggtttg acgattttga aatcaagccg ctggcttctg cttctatcgc
    19021 ccaggttcat accgcgcgat tgaaatcgaa tggtaaagag gtggtgatta aagttatccg
    19081 cccggatatt ttgccggtca ttaaagcgga tctgaaactt atttaccgtc tggctcgctg
    19141 ggtgccgcgt ttgctgccgg atggtcgccg tctgcgccca accgaagtgg tgcgcgagta
    19201 cgaaaagacc ttgattgatg aactgaattt gctgcgggaa tctgccaacg ccattcagct
    19261 tcgacgcaat tttgaagaca gcccgatgct ctacatcccg gaagtttacc ctgactattg
    19321 tagtgaaggg atgatggtga tggagcgtat ttacggcatt ccggtgtctg atgttgcgac
    19381 gctggagaaa aacggcacca acatgaaatt gctggcggaa cgcggcgtgc aggtgttctt
    19441 cactcaggtc tttcgcgaca gctttttcca tgctgatatg caccctggca acatcttcgt
    19501 aagctatgaa cacccggaaa acccgaaata tatcggcatt gattgcggga ttgttggctc
    19561 gctaaacaaa gaagataaac gctatctggc ggaaaacttt atcgccttct ttaatcgcga
    19621 ctatcgcaaa gtggcagagc tacacgtcga ttctggttgg gtgccaccag ataccaacgt
    19681 tgaagagttc gaatttgcca ttcgtacggt ctgtgaacct atctttgaga aaccgctggc
    19741 cgaaatttcg tttggacatg tactgttaaa tctgtttaat acggcgcgtc gcttcaatat
    19801 ggaagtgcag ccgcaactgg tgttactcca gaaaaccctg ctctacgtcg aaggggtagg
    19861 acgccagctt tatccgcaac tcgatttatg gaaaacggcg aagcctttcc tggagtcgtg
    19921 gattaaagat caggtcggta ttcctgcgct ggtgagagca tttaaagaaa aagcgccgtt
    19981 ctgggtcgaa aaaatgccag aactgcccga actggtttac gacagtttgc gccagggcaa
    20041 gtatttacag catagtgttg ataagattgc ccgcgagctt cagtcaaatc atgtacgtca
    20101 gggacaatcg cgttattttc tcggaattgg cgctacgtta gtattaagtg gcacattctt
    20161 gttggtcagc cgacctgaat gggggttgat gcccggctgg ttaatggcag gtggtctgat
    20221 cgcctggttt gtcggttggc gcaaaacacg ctgatttttt catcgctcaa ggcgggccgt
    20281 gtaacgtata atgcggcttt gtttaatcat catctaccac agaggaacat gtatgggtgg
    20341 tatcagtatt tggcagttat tgattattgc cgtcatcgtt gtactgcttt ttggcaccaa
    20401 aaagctcggc tccatcggtt ccgatcttgg tgcgtcgatc aaaggcttta aaaaagcaat
    20461 gagcgatgat gaaccaaagc aggataaaac cagccaggat gctgatttta ctgcgaaaac
    20521 tatcgccgat aagcaggcgg atacgaatca ggaacaggct aaaacagaag acgcgaagcg
    20581 ccacgataaa gagcaggtgt aatctgtgtt tgatatcggt tttagcgaac tgctattggt
    20641 gttcatcatc ggcctcgtcg ttctggggcc gcaacgactg cctgtggcgg taaaaacggt
    20701 agcgggctgg attcgtgcgt tgcgttcact ggcgacaacg gtgcagaacg aactgaccca
    20761 ggagttaaaa ctccaggagt ttcaggacag tctgaaaaag gttgaaaagg cgagcctcac
    20821 taacctgacg cccgaactga aagcgtcgat ggatgaatta cgccaggctg cggagtcgat
    20881 gaagcgttcc tacgttgcaa acgatcctga aaaggcgagc gatgaagcgc acaccatcca
    20941 taacccggtg gtgaaagaca atgaaactgc gcatgaaggc gtaacgcctg ctgctgcaca
    21001 aacgcaggcc agttcgccgg aacagaagcc agaaaccacg ccagagccgg tggtaaaacc
    21061 tgctgcggac gctgaaccga aaaccgctgc gccttcccct tcgtcgagtg ataaaccgta
    21121 aacatgtctg tagaagatac tcaaccgctt atcacgcatc tgattgagct gcgtaagcgt
    21181 ctgctgaact gcattatctc ggtgatcgtg atattcctgt gtctggtcta tttcgccaat
    21241 gacatctatc acctggtatc cgcgccactg atcaagcagt tgccgcaagg ttcaacgatg
    21301 atcgccaccg acgtggcctc accgttcttt acgccgatca agctgacctt tatggtgtcg
    21361 ctgattctgt cagcgccggt gattctctat caggtgtggg cgtttatcgc cccagcgctg
    21421 tataagcatg aacgtcgcct ggtggtgccg ctgctggttt ccagctctct gctgttttat
    21481 atcggcatgg cattcgccta ctttgtggtc tttccgctgg catttggctt ccttgccaat
    21541 accgcgccgg aaggggtaca ggtatccacc gacattgcga gctatttaag cttcgttatg
    21601 gcgctgttta tggcgtttgg tgtctccttt gaagtgccgg tggcaattgt gctgctgtgc
    21661 tggatgggga ttacctcgcc agaagactta cgcaaaaaac gcccgtatgt gctggttggt
    21721 gcattcgttg tcgggatgtt gctgacgccg ccggatgtct tctcgcaaac gctgttggcg
    21781 atcccgatgt actgcctgtt tgaaatcggt gtcttcttct cacgctttta cgttggtaaa
    21841 gggcgaaacc gggaagagga aaacgacgct gaagcagaaa gcgaaaaaac tgaagaataa
    21901 attcaaccgc ccgtcagggc ggttgtcata tggagtacag gatgtttgat atcggcgtta
    21961 atttgaccag ttcgcaattt gcgaaagacc gtgatgatgt tgtagcgcgc gcttttgacg
    22021 cgggagttaa tgggctactc atcaccggta ccaatctgcg tgaaagccag caggcgcaaa
    22081 agctggcgcg tcagtattcg tcctgttggt caacggcggg cgtacatcct cacgacagca
    22141 gccagtggca agctgtgact gaagaagcga ttattgagct ggccgcgcag ccagaagtgg
    22201 tggcgattgg tgaatgtggt ctcgacttta accgcaactt ttcgacgccg gaagagcagg
    22261 aactcgcttt tgttgcccag ctacgcattg ccgcagaatt aaacatgccg gtatttatgc
    22321 actgtcgcga tgcccacgag cggtttatga cattgctgga gccgtggctg gataaactgc
    22381 ctggtgcggt tcttcattgc tttaccggca cacgcgaaga gatgcaggcg tgcgtggcgc
    22441 gtggaattta tatcggcatt accggttggg tttgcgatga acgacgcggg ctggagctgc
    22501 gggaattgtt gccgttgatt ccggcggaga aattgctgat cgaaactgat gcgccgtatc
    22561 tgctccctcg cgatctcacg ccaaagccat catcccggcg caacgagcca gcccatctgc
    22621 cccatatttt gcaacgtatt gcgcactggc gtggagaaga tgccgcatgg ctggctgcca
    22681 ccacggatgc taatgtcaaa acactgtttg ggattgcgtt ttagagtttg cggaactcgg
    22741 tgttcttcac actgtgctta atctctttat taataagatt aagcaatagc atggagcgag
    22801 cctcgccgtc tggctcggtg aaaatggcct gaaaaccttc gaatgcgcct tcagtaataa
    22861 tcactttatc accaggatac ggcgtcgatg gatcgacaat atctttcggt ttatacactg
    22921 ataactgatg aatcaccgcc gatgggacta tcgctggtga cgcgccaaag cgcacgaagt
    22981 ggctgacacc gcgggtcgcg ttgatagtcg tggtatgaat cacttccgga tcgaactcca
    23041 caaacaagta gttggggaac aatggctcac tgactgcagt acgtttgcca cgcacgattt
    23101 tttccagggt gatcatcggt gccaggcaat tcacagcctg tctttcgagg tgttcctggg
    23161 cacgttgaag ttgcccgcgc ttgcagtaca gtaaatacca ggattgcata atgactctta
    23221 tccgtttaat cggggcgcaa ggatagcaaa agctttacgc taagttaatt atattccccg
    23281 gtttgcgtta taccgtcaga gttcacgcta atttaacaaa tttacagcat cgcaaagatg
    23341 aacgccgtat aatgggcgca gattaagagg ctacaatgga cgccatgaaa tataacgatt
    23401 tacgcgactt cttgacgctg cttgaacagc agggtgagct aaaacgtatc acgctcccgg
    23461 tggatccgca cctggaaatc actgaaattg ctgaccgcac tttgcgtgcc ggtgggcctg
    23521 cgctgttgtt cgaaaaccct aaaggctact caatgccggt gctgtgcaac ctgttcggta
    23581 cgccaaagcg cgtggcgatg ggcatggggc aggaagatgt ttcggcgctg cgtgaagttg
    23641 gtaaattatt ggcgtttctg aaagagccgg agccgccaaa aggtttccgt gacctgtttg
    23701 ataaactgcc gcagtttaag caagtattga acatgccgac aaagcgactg cgtggtgcac
    23761 cctgccaaca aaaaatcgtc tctggcgatg acgtcgatct caatcgcatt cccattatga
    23821 cctgctggcc ggaagatgcc gcgccgctga ttacctgggg gctcaccgta acgcgcggcc
    23881 cgcataaaga gcggcagaat ctgggcattt atcgccagca gctaattggt aaaaacaaac
    23941 tgattatgcg ctggctgtcg catcgcggcg gcgcgttgga ttatcaggag tggtgtgcgg
    24001 cgcatccggg cgaacgtttc ccggtttctg tggcgctggg tgccgatcct gccacgattc
    24061 tcggtgcagt cacccccgtt ccggatacgc tttcagagta tgcgtttgcc ggattgctgc
    24121 gcggcaccaa gaccgaagtg gtgaagtgta tctccaatga ccttgaagtg cccgccagtg
    24181 cggagattgt gctggaaggg tatatcgaac aaggcgaaac tgcgccggaa gggccgtatg
    24241 gcgaccacac cggttactat aacgaagtcg atagtttccc ggtatttacc gtgacgcata
    24301 ttacccagcg tgaagatgcg atttatcatt ccacctatac cgggcgtccg ccagatgaac
    24361 ctgcggtact gggtgtagca ctgaacgaag tgttcgtgcc gattctgcaa aaacagttcc
    24421 cggaaattgt cgatttttac ctgccgccgg aaggctgttc ttatcgtctg gcggtagtga
    24481 cgatcaaaaa acagtacgcc ggacacgcga agcgcgtcat gatgggcgtc tggtcgttct
    24541 tacgccagtt tatgtacact aaatttgtga tcgtttgcga tgatgacgtc aacgcacgcg
    24601 actggaacga tgtgatttgg gcgattacca cccgtatgga cccggcgcgg gatactgttc
    24661 tggtagaaaa tacgcctatt gattatctgg attttgcctc gcctgtctcc gggctgggtt
    24721 caaaaatggg gctggatgcc acgaataaat ggccggggga aacccagcgt gaatggggac
    24781 gtcccatcaa aaaagatcca gatgttgtcg cgcatattga cgccatttgg gatgaactgg
    24841 ctatttttaa caacggtaaa agcgcctgat gcgcgtttgt tttgccctat ttatcgatcc
    24901 gacagagaaa gcgcatgaca accttaagct gtaaagtgac ctcggtagaa gctatcacgg
    24961 ataccgtata tcgtgtccgc atcgtgccag acgcggcctt ttcttttcgt gctggtcagt
    25021 atttgatggt agtgatggat gagcgcgaca aacgtccgtt ctcaatggct tcgacgccag
    25081 atgaaaaagg gtttatcgag ctgcatattg gcgcttctga aatcaacctt tacgcgaaag
    25141 cagtcatgga ccgcatcctc aaagatcatc aaatcgtggt cgatattccc cacggagaag
    25201 cgtggctgcg cgatgatgaa gagcgtccga tgattttgat tgcgggcggc accgggttct
    25261 cttatgcccg ctcgattttg ctgacagcgt tggcgcgtaa cccaaaccgt gatatcacca
    25321 tttactgggg cgggcgtgaa gagcagcatc tgtatgatct ctgcgagctt gaggcgcttt
    25381 cgttgaagca tcctggtcta caagtggtgc cggtggttga acaaccggaa gcgggctggc
    25441 gtgggcgtac tggcaccgtg ttaacggcgg tattgcagga tcatggtacg ctggcagagc
    25501 atgatatcta tattgccgga cgttttgaga tggcgaaaat tgcccgtgac ctgttttgca
    25561 gcgagcgtaa tgcgcgggaa gatcgcctgt ttggcgatgc gtttgcattt atctgagata
    25621 taaaaaaacc cgcccctgac aggcgggaag aacggcaact aaaactttat tcagtggcat
    25681 tactctacgt tgcatccggc aaacagtgcc cgatgcaacg ctgccgcgtc ttatcgggcc
    25741 tacaaaagtt ctgaaccgtg ggccggataa ggcgttcacg ccgtatccgg catacagtgc
    25801 ccgatgcgac gctgccgcgt cttatcgggc ctacaaaagt tctgaaccgt agaccggata
    25861 aggcgttcac gccgcatccg gcaagtggtt aaacccgctc aaacaccgtt gcaatgccct
    25921 gacccagacc gatacacatc gtcgccagac caaactggac gtctttgtgt tccatcagat
    25981 tcagcagcgt ggtgctgata cgcgcaccgg aacaacccag cggatgaccc agcgcgatcg
    26041 cgccaccgtt gaggttgatc ttctcgtcaa tctgctccat tagcccgaga tctttaatac
    26101 acggcaggat ctgcgcggca aaggcttcat tcatctcgaa tacgccgata tcgctggcag
    26161 aaagccccgc ttttttcagc gccagcttcg aggccggaac cggaccataa cccataatcg
    26221 atgggtcaca acccacaacc gccatcgaac gcacgcgggc gcgcggctta agacctaatt
    26281 cacgggcgcg gctttcactc atcaccagca tggcagctgc gccatcggaa agcgcagaag
    26341 atgtacccgc cgttaccgta ccgctgacgg gatcaaacgc cggacgcagt gtggcgaggg
    26401 cttccacggt ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc aatacgccgt
    26461 cggcatcgtg accaccggtc gggatgattt catttttaaa tgcgcctgac tgcgtggcgg
    26521 cccacgcgcg ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg ctgataccgt
    26581 gcatacgcgc cagcatttct gccgttaagc ccatcatgcc cgccgctttg gcgacattac
    26641 ggctaaggcc ggggtgaaaa tcgacgccgt gactcattgg cacatggccc atatgctcca
    26701 cgccgccaac cagacatgcc tgcgcatcgc cagtcataat catccgtgct gcgtcatgca
    26761 gtgcctgcat ggatgaacca cacaagcgat taacggtaac cgccgggaca gagtgcggca
    26821 cttcagccaa cagtgccgcg ttacgggcga tattaaaacc ctgctccagt gtttgctgca
    26881 cacaacccca gtaaatatcg tcaagggccg ccgcttccag tgccgggtta cgcgccagca
    26941 ggctacgcat taaatgagcg gagagatctt ccgcacgcac gtgacgaaaa gcaccgccct
    27001 tcgaacggcc catcggggtg cgaattgcat cgacaatgac aacctgttcc attgtgactc
    27061 cttaagccgt tttcaggtcg ccaaccggac gggctggctc aaccggggga tagtacggtt
    27121 cgttatgacg cgctttatca cgcagacctt ccggcacttc atacagcggg ccgaggtgct
    27181 gatattgctg tgccatatcg agatattttg cgctaccgag ggtgtccagc cagcggaacg
    27241 cgccgccgtg gaacggaggg aagcccaggc catagaccag cgccatatcc gcttctgccg
    27301 gagtggcgat aatgccctct tccagacagc ggaccacttc gttgaccatc gggatcatca
    27361 tgcgggcgat aatctcttct tcgctgaaat cgcgcttcgc gtggctgacc tctgccagca
    27421 ggtcgtcaac gacggcgtct tcttctttct tcggcttgcc tttactgtct tctttataac
    27481 gccagaaacc gaggccgttc ttctgaccaa aacggttggc atcaaacagc gcatcgatgg
    27541 catcacggta atctttctgc atccgctgcg ggaagcctgc tgccatcacg gcctgggcgt
    27601 ggtgcgcggt atcaatcccc acgacatcca gcagatatgc cggacccatc ggccagccaa
    27661 actgtttttc catcactttg tcgatcttgc ggaaatccgc gccgtcgcgc agcaactggc
    27721 tgaatccggc gaagtagggg aacagtacac ggttaacgaa gaagccgggg cagtcgttga
    27781 ccacaatcgg cgtcttgccc atcttgctcg cccatgcgac gactttcgcg atggtttcgt
    27841 cggagctttt ctcgccgcga ataatttcca ccaacggcat ccggtgcacc gggttaaaga
    27901 agtgcatccc gcagaagttt tccggacgtt ccagcgcgtt ggccagttcg ctgataggaa
    27961 tggttgaagt gttagacgcc agcacggtat ccgggcgtac tttctgctcg gtttctgcca
    28021 gcacggcttt tttcactttc gggttttcaa caaccgcttc taccacaaca tccacgcggt
    28081 caaatccggc gtagtcgagc gttgggtgga ttgtggagat cacgccagcc agtttcagac
    28141 catcgatctt gccgcgttca agctgcttgt tcagcagttt cgcggcttcg gtcatgccga
    28201 gggttaacga cttgtcgttg atatctttca tgataaccgg cacacctttc cacgcagact
    28261 ggtaagcgat gccgccgccc ataatgcctg cgcccagcac agcggcctgt ttcggcgttt
    28321 caacgtcttt ggtgagtttc ttcgctttgc cttttacata ttgatcgtta aggaatatgc
    28381 cgaccagtgc gcgagcttcg ttggtatgcg ccagcgggac aaaacttttg ttttccaggt
    28441 ttagggcttc ttcacgacca aaacgggccg cagcttcaat ggtttttact gcagtgatgg
    28501 gggccggata atgtttcccc gccgtttgcg cgaccatccc tttagcgatg gtgaagctca
    28561 tggtggcttc aatcttgctc agttttagcg gttccagctt cggctgacgt tttgctttcc
    28621 agtcgaggtc gccgttaatg gcctgacgta aaatcgcctt cgcgccttca accagttttt
    28681 ctgctttgac tacgccatcc accagaccga ttttcagcgc ctgatccgcg ccgacatctt
    28741 taccggcggc aatgatttcc agcgcactgt cagcgcccag catacgtggc atacgtacag
    28801 aaccgccaaa gcccggcatg atgcccagtt tggtttccgg cagaccgatg cgcagatccg
    28861 gcgtcgccag acgataatcg gtcgccagca cgcattcgca gccaccgccc agcgcatagc
    28921 cgttgacggc agcaatggtc ggcaccggca gatcttccag gcgattaaac acgctattgg
    28981 caaagtgcag ccactgactt aactgttctt caggaacgag gaacagggac aaaaattcgg
    29041 tgatatcagc accgacgata aaggctgctt tgttcgaacg tagcagcagt ccttttagat
    29101 ctgattgctg ttccagcacg ccgatggcct cgccgaggct ggcgacggtc gcagtgtcga
    29161 gtttattaac tgaacctggg gtatcaaata ccagttcggt aatgccatct tccagccagt
    29221 caaggtaaag ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc aaggtgatct
    29281 ggtcgtacca gatgcatcga agtgtgtatt ttgtgttaaa aatatgcaaa taaaatatta
    29341 aggaaatgcc gatctgatca cgctcggcag aaatcacgct ctggatgaac gatgtgctaa
    29401 gatgcggaga cttaaggtca aaaaaacaga agggtaaaaa atggaatcac tggcctcgct
    29461 ctataaaaat catatagcta ccttacagga acggactcgc gatgcgctgg cgcgcttcaa
    29521 gctggatgcg ttacttattc actccggcga actgttcaac gtttttctcg acgatcatcc
    29581 ctatccgttt aaagtgaacc cgcaattcaa agcgtgggtg ccggtaactc aggtgccaaa
    29641 ctgctggctg ctggtggatg gcgtgaataa gccgaaactg tggttttatc tgccggttga
    29701 ttactggcac aacgtcgaac cgctgccgaa ctccttctgg actgaagatg tagaagtgat
    29761 cgcgctgcca aaagccgatg gcattggtag tctgttgcct gctgcgcgcg gcaatatcgg
    29821 ttatatcggt ccggtgccag aacgtgcgct gcaactgggt attgaggcca gcaatatcaa
    29881 cccgaaaggg gtgatcgact acctgcatta ctatcgctcc ttcaaaaccg agtacgaact
    29941 ggcctgtatg cgtgaagcgc agaaaatggc ggtcaacggt catcgcgcgg cagaagaagc
    30001 gttccgttct ggcatgagcg agttcgatat caacattgcc tatctgaccg cgaccggtca
    30061 tcgtgatacc gacgtacctt atagcaacat tgtagcgctt aacgaacacg ctgcggtgtt
    30121 gcattacacc aaactggacc accaggcacc ggaagagatg cgcagcttcc tgctggatgc
    30181 cggggccgaa tataacggct atgcggctga tctaacccgt acctggtcgg caaaaagcga
    30241 caacgactac gcacagctgg tgaaagacgt aaatgatgag caactggcgc tgatcgccac
    30301 catgaaagca ggcgtcagct atgtggatta ccatatccag ttccatcagc gcatcgccaa
    30361 actgctgcgt aaacatcaaa tcatcaccga tatgagtgaa gaagcgatgg tcgaaaacga
    30421 tctcaccgga ccgtttatgc cgcacggtat cggccatccg ctgggcctgc aggtgcatga
    30481 cgtagccggt tttatgcagg atgatagcgg tacgcacctc gcggcaccgg caaaatatcc
    30541 gtacctgcgc tgcacccgta ttctccagcc gggcatggtg ttaaccatcg aaccgggtat
    30601 ctacttcatc gaatcgcttc tggcaccgtg gcgtgaaggg cagttcagca aacacttcaa
    30661 ctggcagaaa attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag acaacgtggt
    30721 gattcacgaa aacaacgtgg aaaacatgac ccgggatctg aaactggcgt gatggaaagc
    30781 tggttaattc ctgcggcacc ggtcacggtc gttgaagaga tcaaaaagag ccgcttcatt
    30841 acgctgttgg cgcataccga tggcgttgag gcggcaaaag cgtttgttga atcggtgcgg
    30901 gcagaacacc ccgatgcccg gcaccattgc gtggcgtggg tggcgggtgc gccggatgat
    30961 tctcaacagc tgggtttctc tgacgacggg gagccggcgg gaacggcagg taaaccgatg
    31021 ctcgcccaac taatgggcag cggcgtcggg gaaattaccg ctgtggtggt gcgctactac
    31081 ggcggcatat tgcttggcac cggtgggtta gtgaaagcgt atggcggcgg cgtgaatcag
    31141 gcgctgcgcc agctaacgac ccaacgcaag acaccattaa ccgaatatac tttgcaatgt
    31201 gaatatagtc agttaaccgg cattgaagcg ttgctggggc agtgtgacgg caaaattatt
    31261 aacagtgatt atcaggcatt cgttctgctg cgggtggcgc ttccggcggc gaaagtggct
    31321 gaattttccg caaagctggc ggattttagc cgtggttcat tgcaattgtt agcgattgaa
    31381 gaataatccc cacttcgttt tgcagaacta aggaagcggc agagatgcat tttcgcgcca
    31441 ttacccgaat cgttggacta ctggtcatct tattttcagg gaccatgatt atccctgggc
    31501 tggtagcact catctaccgg gatggagcgg gccgcgcttt tacccagacc ttttttgtcg
    31561 ccctcgccat tggctctatg ctttggtggc cgaaccgcaa agagaagggc gaactgaaat
    31621 cccgtgaggg gtttctgata gtggtgctgt tctggaccgt gctgggtagc gtcggtgcgc
    31681 tcccttttat cttctcggaa agcccgaacc tcacgattac cgatgcgttt tttgaatctt
    31741 tctctggcct gaccaccacg ggggccacta cgctggtggg gctggattcg ctccctcatg
    31801 ccatcctctt ttatcgccag atgctgcaat ggtttggcgg tatggggatc atcgtgctgg
    31861 cggttgcgat actgcctatc ctcggcgtgg gtgggatgca gctctatcgc gcagaaatgc
    31921 ccggcccgct gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg aaaaccctgt
    31981 ggttgattta tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt gctggaatgg
    32041 atgcctttga tgccatcggc catagctttg cgaccatcgc tattggcggc ttctcgacac
    32101 atgatgccag tatcggttat ttcgacagcc cgactattaa cactatcatt gctatcttcc
    32161 tgctgatctc tggctgtaac tacggtctgc acttttcact gttaagtggg cgtagtctga
    32221 aggtttattg gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt tcactggtgg
    32281 ttatttgtac actcgtactg tggtttcata atgtctacag ttcggcgctg atgacaatta
    32341 accaggcgtt tttccaggtg gtgtcgatgg cgacaaccgc cgggtttaca actgacagca
    32401 ttgcccgctg gccgctcttt ttgccggtac tgcttttatg ttcagctttt atcggcggtt
    32461 gcgccgggtc aacgggcggt ggcctgaaag tgatccgcat cctgctgctg tttaagcagg
    32521 ggaaccgtga gctgaaacga ctggtgcatc cgaacgccgt ctatagcatt aagctgggga
    32581 atcgcgcact gccggaacgt atcctcgaag ccgtttgggg atttttctcc gcctatgcat
    32641 tggtgtttat tgtcagtatg ctggcgatta tcgccacggg cgtggatgac ttttctgcct
    32701 ttgcctcggt tgttgcaaca ttgaataacc tggggccagg gcttggcgtg gttgctgata
    32761 actttaccag tatgaacccg gtggctaaat ggatcctgat tgccaacatg ctgtttggtc
    32821 gtctcgaggt ctttacattg ctggtgctct ttaccccgac tttctggcgt gaatgatgga
    32881 gtaatacgtg aaaacattaa ttcttttctc aacaagggac ggacaaacgc gcgagattgc
    32941 ctcctacctg gcttcggaac tgaaagaact ggggatccag gcggatgtcg ccaatgtgca
    33001 ccgcattgaa gaaccacaat gggaaaacta tgaccgtgtg gttattggcg cttctattcg
    33061 ctatggtcac taccattctg cgttccagga atttgtcaaa aagcatgcga cgcggttgaa
    33121 ttcgatgccg agtgcctttt actccgtaaa tctggtggcg cgtaaaccgg agaagcgtac
    33181 tccacagacc aacagctacg cgcggaagtt tctgatgaac tcgcaatggc gtcccgatcg
    33241 ctgtgccgtc attgccgggg cgctgcgtta cccacgttat cgctggtacg accgttttat
    33301 gatcaagctg attatgaaga tgtcaggcgg tgaaacggat acgcgcaaag aagttgtcta
    33361 taccgattgg gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa ccgacaaacc
    33421 gacgctgaaa taagcataaa gaataaaaaa tgcgcg
//
LOCUS       DADTFG010000038        33040 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.038, whole genome shotgun sequence.
ACCESSION   DADTFG010000038 DADTFG010000000
VERSION     DADTFG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.038"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(75..1388)
                     /gene="gltP"
                     /locus_tag="J1138_001674"
     CDS             complement(75..1388)
                     /gene="gltP"
                     /locus_tag="J1138_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBA8453483.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1731..2327)
                     /gene="nrfG"
                     /locus_tag="J1138_001675"
     CDS             complement(1731..2327)
                     /gene="nrfG"
                     /locus_tag="J1138_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBA8453484.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRTEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQTRAMIDRALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(2324..2707)
                     /gene="nrfF"
                     /locus_tag="J1138_001676"
     CDS             complement(2324..2707)
                     /gene="nrfF"
                     /locus_tag="J1138_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBA8453485.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIDWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(2700..4358)
                     /locus_tag="J1138_001677"
     CDS             complement(2700..4358)
                     /locus_tag="J1138_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBA8453486.1"
                     /translation="MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCF
                     LISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTD
                     PLFPLTLAVLSLMLATLLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
                     LGYGGLMVAASVALASLLRGELDGASARICWRWALPGWSALTAGIILGSWWAYCELGW
                     GGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIV
                     RSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGGPAVRFSGLSREMLI
                     LATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLAT
                     FVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLD
                     LQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMG
                     EKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE"
     gene            complement(4438..5394)
                     /gene="nrfD"
                     /locus_tag="J1138_001678"
     CDS             complement(4438..5394)
                     /gene="nrfD"
                     /locus_tag="J1138_001678"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBA8453487.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRVYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(5391..6062)
                     /gene="nrfC"
                     /locus_tag="J1138_001679"
     CDS             complement(5391..6062)
                     /gene="nrfC"
                     /locus_tag="J1138_001679"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBA8453488.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPSSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(6059..6625)
                     /gene="nrfB"
                     /locus_tag="J1138_001680"
     CDS             complement(6059..6625)
                     /gene="nrfB"
                     /locus_tag="J1138_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBA8453489.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(6670..8106)
                     /gene="nrfA"
                     /locus_tag="J1138_001681"
     CDS             complement(6670..8106)
                     /gene="nrfA"
                     /locus_tag="J1138_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBA8453490.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            8498..10456
                     /gene="acs"
                     /locus_tag="J1138_001682"
     CDS             8498..10456
                     /gene="acs"
                     /locus_tag="J1138_001682"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBA8453491.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            10759..11073
                     /locus_tag="J1138_001683"
     CDS             10759..11073
                     /locus_tag="J1138_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBA8453492.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNKVLHEVQ
                     AS"
     gene            11070..12719
                     /gene="actP"
                     /locus_tag="J1138_001684"
     CDS             11070..12719
                     /gene="actP"
                     /locus_tag="J1138_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBA8453493.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(12758..13447)
                     /locus_tag="J1138_001685"
     CDS             complement(12758..13447)
                     /locus_tag="J1138_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBA8453494.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(13440..13850)
                     /locus_tag="J1138_001686"
     CDS             complement(13440..13850)
                     /locus_tag="J1138_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBA8453495.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            13954..14838
                     /locus_tag="J1138_001687"
     CDS             13954..14838
                     /locus_tag="J1138_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8453496.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAKHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(14874..16523)
                     /locus_tag="J1138_001688"
     CDS             complement(14874..16523)
                     /locus_tag="J1138_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBA8453497.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(16674..18023)
                     /locus_tag="J1138_001689"
     CDS             complement(16674..18023)
                     /locus_tag="J1138_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA8453498.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(18569..19033)
                     /gene="soxR"
                     /locus_tag="J1138_001690"
     CDS             complement(18569..19033)
                     /gene="soxR"
                     /locus_tag="J1138_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBA8453499.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            19119..19442
                     /gene="soxS"
                     /locus_tag="J1138_001691"
     CDS             19119..19442
                     /gene="soxS"
                     /locus_tag="J1138_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBA8453500.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(19478..21031)
                     /gene="pdeC"
                     /locus_tag="J1138_001692"
     CDS             complement(19478..21031)
                     /gene="pdeC"
                     /locus_tag="J1138_001692"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBA8453501.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHTDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKHGVRYCQGWFFAKAMP
                     PQVFMQWMG"
     gene            21460..21741
                     /locus_tag="J1138_001693"
     CDS             21460..21741
                     /locus_tag="J1138_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453502.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(21840..22376)
                     /gene="ssb1"
                     /locus_tag="J1138_001694"
     CDS             complement(21840..22376)
                     /gene="ssb1"
                     /locus_tag="J1138_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBA8453503.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            22631..25453
                     /gene="uvrA"
                     /locus_tag="J1138_001695"
     CDS             22631..25453
                     /gene="uvrA"
                     /locus_tag="J1138_001695"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBA8453504.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(25488..25844)
                     /locus_tag="J1138_001696"
     CDS             complement(25488..25844)
                     /locus_tag="J1138_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBA8453505.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(25848..26264)
                     /locus_tag="J1138_001697"
     CDS             complement(25848..26264)
                     /locus_tag="J1138_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBA8453506.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(26375..27088)
                     /gene="aphA"
                     /locus_tag="J1138_001698"
     CDS             complement(26375..27088)
                     /gene="aphA"
                     /locus_tag="J1138_001698"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBA8453507.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            27365..29185
                     /locus_tag="J1138_001699"
     CDS             27365..29185
                     /locus_tag="J1138_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA8453508.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWSEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCFTVSNFTFLKDKKEQFCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE"
     gene            29178..30527
                     /locus_tag="J1138_001700"
     CDS             29178..30527
                     /locus_tag="J1138_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBA8453509.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPE"
     gene            complement(30471..31514)
                     /locus_tag="J1138_001701"
     CDS             complement(30471..31514)
                     /locus_tag="J1138_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HBA8453510.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSI
                     NLRLNLMFVEFVI"
     gene            complement(31526..33025)
                     /locus_tag="J1138_001702"
     CDS             complement(31526..33025)
                     /locus_tag="J1138_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HBA8453511.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSAILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
BASE COUNT         8452 a         8822 c         8230 g         7536 t
ORIGIN      
        1 tcacgctaca tcaggctatt actcagtcag gcatccacac attgccgggt ggatatcccc
       61 ccggcaatct tcaattactg atccgccgtt ttatcaaatt tgcccagcac ttcacgctca
      121 taagccagcg ctttcttacg gtcaaatttg tgttcccact tggcaatcac cagcaccgcc
      181 agcgcattac ccaccacgtt cagcgcagta cgcgccatat cgaggatacg gtcaacaccg
      241 gcaataaacg ccagaccttc cagcggaata cctacgctac ccagcgttgc cagcaacacc
      301 acaaacgaca cgcccggcac gccagcaatc cctttcgagg tcaccatcag cgtcagcacc
      361 agaatgattt cctgccagat ggacaggtca atgccataca gctgagcgat gaaaatagcg
      421 gcaatacttt gatacagcgt cgaaccatca aggttaaaag agtaaccggt cggcaccacg
      481 aaactggtga tcgacgccgg tgctccgtag gcttccatct tctcaataat tcgcggcagc
      541 acgctttcag agctggcagt ggagtacgcc agaatcagct catctttcag aatacgaatc
      601 aggatccaga cgcttaaccc gcacaggcgc gccacaattc ccagcactac cagcgcgaag
      661 aacagaatgg cgaaatgcac cagcagcacc agtttcgcca gtggccacag agacgagaaa
      721 ccaaagttag ccaccgtcac cgcaatcagc gcaaacacac ccaccggcgc ataacgcatc
      781 accatgtgag tcactttaaa catggtttca gagatggagc ggaaaacggt caccagcggt
      841 tcacgatgcg tcgcgggcag ggatgaaagc cccagaccaa acagcaccga gaagaagatg
      901 atcggcagca tttcgccttt cgccatcgac gccacgatgt tcgtcggcac cagcgacaaa
      961 atcgtgccca taatgccgtg ggaactgctt tgtaccgctt ccgtagtgct ctgatatttc
     1021 gagatatcga cggtcgccag ctgcgacata tccaccccgg caccgggctg gaagacgttc
     1081 gccagagtga tccccaaaat gatggcgacg gtggtgatca cctcgaagta gataatggtt
     1141 ttcgcaccaa tacgcccgag ctgtttggca tcaccaacac ccgcgatacc caccaccagt
     1201 gtggagatca caatcggcac aacaatcatt ttgatcagat ggatgaagat atcacccgcc
     1261 ggagagagca aattgacgac cagccagtcg cggctgtcgc tatggtagtg caggtagctt
     1321 cccaggagaa tgcccagcac catagcaaac agaatctgcc aggccaggct gaattttata
     1381 tttttcatca gaataacttc ctcaatggag cgccgttccc cgttcagaaa cgcgagaacg
     1441 tgacatactg ccagggcatg aattgtgttg cgttggtttg tagatttttt taacgcttat
     1501 gatcagtatc atttgcgcgg tatgttgcgc aaccctttaa caacagagct tttcgctgca
     1561 ttaatgcata ataacgcgag tttatggcag gattaatgaa taaccatttg ttatagaagg
     1621 atttttattt gtttaatctg caataatact gattggtgat tatttttgat tatatgttca
     1681 ttcgattatt ttttatgcag ctcaataaca taatcttcat caccagatga ttaatctgat
     1741 ctccgctgca acaatttcgc catattaatc gactcgacca gctgtgtccg gttgatccgg
     1801 ggtgagttga gatccatcac tttttgccat agttcgatgg cttgcgcgta gttcgcctgc
     1861 ataaacgcat cggaagccag cagcatcaga gcggtgattt cgttagagtc cagcgcgagg
     1921 gctctgtcga tcattgcgcg agtctggacg gtcatatgtt ggctggcctg gtaatacagc
     1981 accgtcgcca gcgccgcata cagttcagcg ttctcaccac gtagttgcaa cgcctgacgg
     2041 tacgccagca gcgaattgct gtaatcgttt tgccacagat aatactcgcc cagtaacgcc
     2101 cactgttcgc tgttttgtgg attagcacgg attttatcct gcaatgcctg aagctgcgct
     2161 tccggggttt gctggctggc aaactgatgc agcggatcgc gctgacgctg atactccgta
     2221 cgtacagcct gccattttgg acttaacaga taactgccga cacagagaaa taccatcaaa
     2281 atcgtgagtg ttgtcagcat tttcaccggt atttggggct gtttcatcgc ttcgccctca
     2341 ctcgccagag gatcagtgcc atcagcagta acaacactac tggcagcgcc cacaacacca
     2401 gcgtctgacc cgttaacggt gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc
     2461 agtcaatgat ttcgacttca cttttcccct cttccaccat ggtgtaaacc tgatggcgca
     2521 tactgactgc caccggcgca ttggattcca gtaagttctg attctggcac tgcggacaac
     2581 gtaactggct ggcaatattt aacgcctgtt gctgttgttg cggattggcg aattgccagg
     2641 tgtctacaac ctgagcgcgc gcaaaacagg taaataacag cagtagcgtg agaagccctt
     2701 tattcatcgc gcttccttcc ccgccatccg cttaacaatg cgcccgcaat catcaacaat
     2761 cctccccccc agatccagcg cacaccgctt tgtacataca aacgaaaagc gtaacgatcc
     2821 gctccggttt tctcgcccat gaccgcatac cagtcatgga tgccattcca gcgaattgac
     2881 ggttccatca tttgctgacg acgtgcttcg taaaaacgcc gctccggcgt taattcacca
     2941 atgcgttgct gatggtcaaa cagtgccact atcgcttttt cgctggtgta attgcctttg
     3001 gcttgcagat cgaggcgctc aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc
     3061 ggctgtaaat tcaggctgat ctcctgacgg ctgacgctgg aaaccacgat ccccgcggca
     3121 aataacagca cgcccgcatg cgccagcagc gccggaagct gagcacgttt gccagagaca
     3181 aacgtcgcca gcacaatcac caccagcatc aacagagcaa acggtaacgt cgcgcggtta
     3241 aaatacggcg cgccaacgga gaggcgtccc cagcccagca ggccgtaaat catcggataa
     3301 agcgttccca ccagcacgat cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt
     3361 aacatttccc gcgataaccc cgaaaaacgc accgccgggc caccgtcccg cgctcgccag
     3421 ccatacagag ccagagacgc aaggctaatc agtgcaaaca ggctgaacaa cggcacggcg
     3481 cggacgttat ccagcgcgaa cgcatgaacc gaaaccagaa tgccagaacg gacaattaag
     3541 gtgcccagca gcgacagcat cagagttact atcgccagca acagcgacca gtggcggaaa
     3601 atcccccgct ggcgtgtcag ggataaactg tgcagcagcg ctgtggcaga aagccagggt
     3661 aataacgacg cgttttccac cggatcccag aaccaccagc cgccccagcc cagttcgcaa
     3721 taggcccacc aggaaccgag gatgatcccc gccgttaatg cgctccagcc aggtagtgcc
     3781 caacgccagc aaattcgggc gctggcacca tcaagctcgc cgcgcagtaa actcgccagt
     3841 gccacgctcg ccgccaccat caaaccgccg tagccgagat aaagcagcgg tggatgaaag
     3901 ataagaccgg gatgttgcag catcggattg agatcgcggc cttcgattgc tggtggaaat
     3961 atccgcacga agggatcgga ccacagcacc acaaacagta gcagtgtggc gagcatgaga
     4021 gataaaacgg ctagcgtcag cggaaatagc ggatcggttt gctgtcgata atgccaggca
     4081 aacagtgcgc tccaggcgga aagcagcagc acccaaagca gcagcgaacc ttcatgaccg
     4141 ccccacaccg ccgccagttt gagttcccac gacagcaggc tatagctatg ttgggcgaca
     4201 taaatgaccg agaaatcgct gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc
     4261 aggagcgcga actgcgccag aatgccgatg caagtgagtc gcatcatggc aggccagcgc
     4321 aaccgcactc ccgcgaaggc cgtcaacggg gtcaacacgt tgaccccgag acttaacaac
     4381 aacgccagaa aactgacttc aggaaggaat acgtccagaa accacctctg ttctggctta
     4441 cgccaccgtt aactgcccgg cgtagagaat gaaaaagcgc agcatcaaca cgccgaccag
     4501 gctggcccca catgccgcca gcacggcagg aatgccggaa ctgcgattga cccacggttt
     4561 gagcaacatt ggcacaatca gccccagccc ggcgacacca agccagaacc accacgtcca
     4621 gaaaccgccg cccaatgccg ccaccagcgc acgcacttta ccgtcatcgc ccagcgccag
     4681 accgacaaaa aacgccacca gcaggaagat ttcaccccat accacgggga tttccatacg
     4741 gtgtacaaag tgcgcttccg tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat
     4801 cagcgccacc gccgcaccag acgagatgcc ggagaagagg aacagcaccg gcaggatcgg
     4861 gttattgagg aacggatacg atttcagcgc cgacagcaga aagccggtat aagcccccaa
     4921 caacaccgcc agcaccagca tcaatgtttc cagtccgcga tgaacgggcg tcagcaggct
     4981 caagaccttt tgcacgatcc ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc
     5041 atgttcaaag attttcgcca gccacagcac cagcaccacc atgtaaagct gaaacagcat
     5101 cacccccatc gacatcaccg aggtaaaact gtagtggaac atcagcttcc agaaggtcca
     5161 cggtcttgtc aggtggaaga cgaggatcaa cagaccgagg atcaccgcgc caggcccgac
     5221 aatcagcgtg gtgcgcaaca acgtactgtc tgcaccgccc gcctgcgggt agacacgacg
     5281 taacagcacg gccagcgtca ccagaccggc agaaatacca atcaaaaaca ggtagatggc
     5341 aatcggccag tcccacacca gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc
     5401 acctccccgt atttaaacgg tacgcggtac agcttcggtt tggttcccag cgccagcttg
     5461 tagcggtaag tgggcttctg gcgcagcagt tgcgaaatct cactgctggg atcgtccaga
     5521 ttgccaaacg tcagcgcctt cgtcgggcaa gcctcaacgc acgcgggcag cttaccggcc
     5581 tgcaaatggg tcttacggca gaaatcgcat ttgtccgccg tcttcgtgac cggatggata
     5641 aagcgcacgc ggtacggaca ggcggcgatg cagtactgac aaccgacaca gagatccgga
     5701 ttaacatcga caatcccact ggcagcgtcg cgaaaagacg caccggtcgg gcaaacgtca
     5761 acgcacggcg catgatcgca gtgctggcaa gacttacgga agaagcgata tttcacatca
     5821 ggaaattcgc cctgcggctc gctacgaata atcgtcaggc gcgagacgcc ttccggcacc
     5881 ttgttcactt cccgacaagc atccatacag gcggtacagc cgatgcataa cgactcgtca
     5941 tgcaccatgc cgtaacgcac cccattgata ttcaacgttt tcgccaccac gcgtcctgcg
     6001 gtgccgctga cggcggccag cacgccgacg ccggtgagaa actggcgacg agaccaggtc
     6061 atggctgctc cttaagcaac ggaacagacg ccgggttaaa gttcggattg gtgcgctgat
     6121 cgctgtggca atcgacgcaa atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat
     6181 cttgctgtgg atgcagggaa tggcagctgg cgcacgccac tttggttacg tggacatcgt
     6241 gcggccagaa cgctttttgc aactgttccg gcagatggca ggacatacag acgctgttct
     6301 gctctccaac cttgtacatc ggctcgttaa agcgcatcac atccttcacc ccttcgcggt
     6361 gttgcggtga tggctgaccg tggcagttgg tgcaggtgac cggcagtttg ttattcgggt
     6421 tgataacaga agcatgtttg ccatgcatac cttcggtatc tggcttatga cagtccagac
     6481 aggcggcatc cgggttacgc tgctgggtaa cttcgtagcg atcatcagat gcctgcgccg
     6541 caggggtagc ggtaattccg ttcaggctcc acaacaggcc cgacgccagc accccggcag
     6601 ttaataacga acgtaatacg ctcatattca ctccattgtg ttttccgccc cttgcgaggc
     6661 ggaacggggt tattggctta acagaccgtt tttacgtgcc tgctcttccc actgcgggat
     6721 caccgttttg atgaagtcct gcttctcggc cttgatttgc tccatgttca ggccaatggc
     6781 ctgctgggct ttctctttgg tcgagatatc cgggatctgg atttcatggg ttatgccttt
     6841 ggtcgccagc aggcgcgcca gtttggtgcg tgcatccgcc gctttatcca tcgccgtacc
     6901 gagcatcctc agaccttctt ccggtgcgtg catatgaatg ccgtgggaag cgatcgccaa
     6961 atcccaacgc cactgggcat gacggatatc gtcctgaatc ggcttcattt cggcttccgt
     7021 tgcgcctgca tccagcgccg ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac
     7081 cttgattttc aggtcgttaa tcgactgctt acgttccgcg accacttttt gcaaggcagc
     7141 tttgtcctgg gtatggcagt tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat
     7201 tttatggtcg gtgtagagtt tgccttcggc gttctgcact tttggcatat ggcaatcgat
     7261 acaggtcacg ttgtttttgc cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg
     7321 cgctttcagc attggcgttt tcgacaggga gttagtccag tcagagaagg caattttgtc
     7381 gtaatactgc tccatatttt cgactttcat gccgtcatcc cacgggaatt taaccgcttt
     7441 gtttttgccg tcgaagtaat actccacatg gcactgaccg caaaccatcg attgctggtc
     7501 gaaacgtccg gctttctcga aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg
     7561 acgggaaagg gttaactccg gtttgccttt ggcgaactct ggtgaggcgg tgttatggca
     7621 atcggcacaa cctaagttgt tgacgatttc cgggccgccg cgcgcccatt ttccgtggaa
     7681 gtagccatct tcgccgtctt tctggatcag acgcgccaca tccgggcttt tacaactcca
     7741 gcacgccatc ggtagcggac catcttcagc gtttttcggc gcaccggtac gcagggtttc
     7801 acgcacatcg gtcacagcaa aggcatgtcc acgcggcttg ttgtaatcgc gcgagaaggg
     7861 ataccctgcc cacaggatca ccagccgtgg atcttccgcc agggcgtcaa cacgctctga
     7921 ctgttccgag gtagctttcc aggagagata ttgatcggga tgctgcgggg caaaggtttc
     7981 attcttcgct tccacagtta caggtgttgc gggagcagcc gtttgttcag cgtgaacaga
     8041 agtgaaaaag aaaaaaggaa tcaataagct gaagatacgg cgtgcgttta tttttatcct
     8101 tgtcataggg cttcatccga attgcgccat tgttgcaatg gcggttttta ttgtttttca
     8161 cgacagtaac cgcacctaca ctgtcatgac attgctcgcc cctatgtgta acaaataacc
     8221 acactgtgaa tgttgtcttt aatcaattgt aagtgcatgt aaaataccac tttagagtta
     8281 gtcagtatct tcctcttttt caacagcatg cataactgca tgttcctcaa agaattaatc
     8341 aacttttgtt gctgaccttc aaaaattacc ctgccgttta tttgcacaat tctacttttg
     8401 cgtgatctgt cgcccaaata ctaaacaaaa ctgccaatac ccctacattt aacgcttatg
     8461 ccacatatta ttaacatctt acaaggagaa caaaagcatg agtcaaattc acaaacacac
     8521 cattcctgcc aacatcgcag accgttgcct gataaaccct cagcagtacg aggcgatgta
     8581 tcaacaatct attaacgcac cagatacctt ctggggcgaa cagggaaaaa ttctcgactg
     8641 gatcacaccg taccagaagg tgaaaaacac ctcctttgcc cccggtaatg tgtccattaa
     8701 atggtacgag gacggcacgc tgaatctggc ggcaaactgc cttgaccgcc atctgcaaga
     8761 aaacggcgat cgtaccgcca tcatctggga aggcgacgac gccagccaga gcaaacatat
     8821 tagctataaa gagctgcacc gcgacgtctg ccgcttcgcc aataccctgc tcgaactggg
     8881 cattaaaaaa ggtgacgtag ttgcgattta tatgccgatg gtaccggaag ccgcggtcgc
     8941 gatgctggcc tgcgcccgca ttggcgcggt acattcggtg attttcggcg gcttctcgcc
     9001 ggaagccgtt gccggacgca ttatcgactc cagttcacga ctggtgatca cttccgacga
     9061 aggtgtgcgt gccgggcgta gtattccgct gaagaaaaac gttgatgacg cgctgaaaaa
     9121 cccgaacgtc accagcgtag agcatgtggt ggtactgaag cgtactggtg gaaaaattga
     9181 ctggcaggaa gggcgcgacc tgtggtggca cgacctggtt gagcaagcca gcgctcagca
     9241 ccaggcggaa gagatgaacg ccgaagatcc gctgtttatt ctctatacct ccggttctac
     9301 cggtaagcca aaaggcgtgc tgcacactac cggcggttat ctggtgtacg cggcgctgac
     9361 ctttaaatat gtctttgatt atcatccggg cgatatctac tggtgcaccg ccgatgtggg
     9421 ctgggttacc ggacacagtt atttgctgta tggcccgctg gcctgcggcg cgaccacgct
     9481 gatgtttgaa ggcgtaccga actggccgac gcctgcccgt atggcgcagg tggtggacaa
     9541 gcatcaggtc aatattctct ataccgcgcc cacggcgatt cgcgcgctga tggcggaagg
     9601 cgataaagcg atcgaaggca ccgaccgttc gtcgctgcgc attctcggtt ccgtgggcga
     9661 gccaattaac ccggaagcgt gggagtggta ctggaagaag atcggcaacg agaaatgccc
     9721 ggtggtcgac acctggtggc agaccgaaac tggcggtttc atgatcacgc cactgcctgg
     9781 cgctaccgag ctgaaagccg gttcggcaac acgtccgttc ttcggcgtgc aaccagcgct
     9841 ggtcgataac gaaggtaacc cgctggaggg ggccaccgaa ggcagtctgg tgatcaccga
     9901 ctcctggccg ggtcaggcgc gtacgctgtt tggcgatcac gaacgttttg agcagaccta
     9961 cttctccacc ttcaaaaata tgtatttcag cggcgacggc gcgcgtcgcg atgaagatgg
    10021 ctattactgg atcaccgggc gcgtggacga cgtgctgaac gtctccggtc accgcctggg
    10081 gacggcagag attgagtcgg cgctggtggc gcatccgaaa attgccgaag ccgctgtggt
    10141 cggtattccg cacaatatta aaggtcaggc gatctacgcc tacgtcacgc ttaatcacgg
    10201 ggaggaaccg tcaccagaac tgtacgcaga agtccgcaac tgggtgcgta aagagattgg
    10261 cccgctggcg acgccagacg tgctgcactg gaccgactcc ctgcctaaaa cccgctccgg
    10321 caaaattatg cgccgtattc tgcgcaaaat tgcggcgggc gataccagca acctgggcga
    10381 tacctcgacg cttgccgatc ctggcgtagt cgagaagctg cttgaagaga agcaggccat
    10441 cgcgatgcca tcgtaaccca caattgccgg atgcgctccg ggcaacaagt cggaaacgat
    10501 ttgtaggcct gataagacgc gacagcggcg catcaggcgc tggttgccgg atgcggcgtg
    10561 aacgccttat ccggcctaca ttcggcaacg gctgtaggcc tgataagacg cgacagcgtc
    10621 gcatcaggcg ttgattgccg gatgcggcgt gaatgcctta tccggcctac attcggcaag
    10681 ggttacccaa gcgttaacct tctcccataa gggagcggga attaaaacaa tccctacatt
    10741 acctctggag aatctgtgat gaatggtacg atttatcagc ggatagaaga caatgcgcat
    10801 ttcagggagt tagtcgaaaa acggcaacgg tttgccacca tcctgtcgat tattatgctg
    10861 gcagtttata tcggctttat tttactgatc gccttcgcgc ccggctggct gggcaccccg
    10921 ctgaatccga acaccagcgt cacacgcggt attccagtcg gtgtcggggt aattgtgatc
    10981 tcctttgttc tcactggtat ctacatctgg cgggcgaacg gcgaattcga ccgtcttaat
    11041 aacaaagtcc tgcatgaggt acaagcatca tgaaaagagt tctgacggcg cttgccgcca
    11101 cactcccttt cgcagctaac gccgcggatg ctattagcgg ggccgtagag cgccagccaa
    11161 cgaactggca ggcgattatt atgttcctga ttttcgtcgt gtttacgctc ggcattacct
    11221 actgggcgtc aaaacgcgta cgttcgcgta atgactacta caccgcaggc ggcaatatca
    11281 ccggttttca gaacgggctg gcgattgccg gggactatat gtccgccgcc tcattcttgg
    11341 ggatctccgc gctggtgttt acctccggct atgacggctt aatttactcg ctaggcttcc
    11401 tggtaggctg gccgatcatt ttgttcctga ttgccgaacg tctgcgtaac ctcggtcgct
    11461 acacctttgc cgatgtggcc tcttaccgtc tgaaacaggg gccgattcgt attctttcgg
    11521 cctgtggttc tctggtggtg gtggcgcttt accttatcgc ccagatggtg ggcgcaggta
    11581 aactgatcga gctgctgttt ggccttaact atcacattgc ggtagtgctg gtcggcgtgc
    11641 tgatgatgat gtacgtcctg ttcggcggca tgctggcgac cacctgggta cagattatca
    11701 aagccgtgct gttgctgttc ggtgccagct ttatggcctt tatggtgatg aaacacgtcg
    11761 gctttagctt caacaatctg ttcagcgaag cgatggcggt acacccgaaa ggtgtcgaca
    11821 tcatgaagcc gggcgggctg gtgaaagatc ctatctccgc gctctctttg gggctaggac
    11881 tgatgtttgg tacggcgggc ttgccgcata ttctgatgcg cttctttacg gtcagcgatg
    11941 cccgcgaagc acgtaagagc gtgttctacg ccaccggatt tatgggctac ttctacatcc
    12001 tgacctttat tatcggcttt ggcgcgatca tgctggtcgg tgcgaatccg gaatataaag
    12061 acgcggcggg ccatctgatt ggtggtaaca acatggcagc agttcacctg gccaatgcgg
    12121 tgggcggcaa cctgttcctc ggctttattt cagcggttgc tttcgccacc attcttgcgg
    12181 tggttgcggg tctgacgctg gcgggcgcat cggcggtgtc tcacgacctg tacgctaacg
    12241 tcttcaaaaa aggtgcgacc gaacgtgaag agctgcgggt atcgaaaatc accgtactga
    12301 tcctcggtgt gattgccatt atcctcggcg tgctgtttga gaatcagaac atcgccttta
    12361 tggtggggct ggcgtttgcc atcgcggcga gctgtaactt cccgatcatt ctgctttcca
    12421 tgtactggtc gaaactgact acccgtggcg caatgctggg cggctggtta ggactgatta
    12481 ctgcggtggt gctgatgatc ctcggtccga cgatttgggt acagatcctc ggtcacgaaa
    12541 aagccatctt cccgtatgaa tacccggcgc tgttctctat cagcgtggca ttcctcggta
    12601 tctggttctt ctcggcaacg gataattcag cggaaggtgc gcgcgagcgt gaactgttcc
    12661 gtgcgcagtt tatccgctcc cagactggct ttggcgttga gcaaggccgc gcgcattaat
    12721 ctctcccctt ccccggtcgc ttgatcgggg aatttcttca gaacatcatc ctggccacca
    12781 gcggcgaaac caataccatc aatacacctg aaagcatcat gaccaggctt gcaactacgc
    12841 cttcttgttg acccagttca tacgaccgcg cagtcccggc accatgagat gccgcgccga
    12901 atccagcacc tttcgccatc ccttcgcgaa tagagagacg taaaaacaac acgtcgccaa
    12961 tcgccatgcc aaacacaccg gtgaccacca caaacagcgc caccagatca ggctgtccgc
    13021 cgagcgactg gctcgctgcc aaagcaaatg gcgtagtgac cgaacgcacc gccaggctac
    13081 gctggatctc atctggcagg gtaaacaatc gcgccagcca cactgagctg gtcactgcca
    13141 ccaccgttgc cgtcagtacg cctgccgtca acgacatcca gtgacgctta atgatcgccc
    13201 gattgtcata cactggtacc gcaaaggcga tggtggccgg acctaatagc catagtaacc
    13261 agtgcgcttc gccaatatag ttttgccagg aaatattgcc aaaaaccagc agcataacca
    13321 gtagcgtagg cgtcagcacc agaggcatca atggcaattt acgaaagcgg cgataaaggc
    13381 gtttgttaag aaaatagata actagcgtaa ttgccagaca gagcatactt agctgaaaat
    13441 tagccacggt ttaacctgcg catttcatag cgatagactt tttccactac ccaggcagtc
    13501 gtgcccagca ccattaccgt actcagcgcg atcacagcaa aaatacgcca gccatccacc
    13561 agtaatagtt gtgcatagtt caccacagcc accacggcag ggacaaaaaa cagcaacatt
    13621 tcggccaata gccaacgcgc cccggctcga acccattgca gaggaagaat gcgacaaaca
    13681 atcagcgcca gcatcaggat catccccacc aggttggcag gcagcggcaa atgcaaccag
    13741 gagacgagat actgcgcaaa aataaataaa cctgcataca gcacaacctg aaaaaatacc
    13801 tggatagatt gcactacggc aggggtaatg cggcgaagcg ccacgggcat gagaggatat
    13861 cctgaaaata aaacagaatg gtaagtatag agagcgctcg cccctgactg aaatgaatta
    13921 aaatcattgc aggtatagtc aaagggaata gttatggata tcagaacgct gcgttatttt
    13981 gtcgaagtcg tgcgccagca aagcttcacc cgcgcagcgg agaagttgtt tgtcacccaa
    14041 ccaactatca gcaagatgct gaagaatctc gaagatgagc tgaactgtat attgttgatc
    14101 cgcgatggtc gtcggctgct tctcactgat actggtcgcg tcgtatttga gcgcgggctg
    14161 gctttactgg ctgagtttcg gcaactggaa gctgagctta gcgatatcaa tcagcttaaa
    14221 accggcgttt tgcggctggg aatcccgccg atggttggga tgttgatggc cgggccgatt
    14281 ggcctttttc gccagcgtta ccccggcgta gaattgaaaa tttcggagtt tggtggtctg
    14341 acggtacaac aagcggtgat gaacggtgaa ctggatatgg cactgacagc cctgccagta
    14401 gaagaaagcg gcctgacaac gttatcgctg tttagtcatc cactatgtgt actgacgcca
    14461 cattcaggta aatggcttga atgtgatgcc gtctcaccgg agacattggc gaaacatcct
    14521 ctacttatct ataacgaaga ttttgcctta agtaagcaat tgatgaagtt attcgatgaa
    14581 catggcgtta agccgagaat tgcagttcgc agcgggcaat gggattttct cgccgcgatg
    14641 gttcaggccg gtgtcggaat agccatattg ccggaaccca tttgccagcg attagataaa
    14701 aacaccttaa aatggctacc gctggaaagc gaactaagct ggaaattagg aatgatttgg
    14761 cgtgagggcg tttacctttc ccactctgcc cacgcgtggc tgagctgttg tgaaggatac
    14821 tggttaacgc cagagtaagt cgggttggcg ggctcgcccg ccaaccatgc agattactga
    14881 ttttcctcaa tcagcaacgc ttcaagcaaa tcgagatcgt gcagtaattt ttgcagcgtt
    14941 tcgttactga tctcccgcgt ggcgcgcagg tggtacagtt cagcacgttc agaacgcaat
    15001 gccgccagac ggaagcgacg ctccaggttc tcttccagga ttgaactttc tacatcatta
    15061 cgtccatcgg cgcgacgacg caggttacca atgacgcgag aactaacctc tgtgagcagc
    15121 tggttatcga tattctcttc ggtatccgcc gccaaacgct cctccatttt ctggatcgcc
    15181 acaatcgcca cttctgccgt tgccgctcgt gcaatacgtt cctctttcaa ttgttgcgaa
    15241 tggtccgcga cttctatgtg ttgtagcaga ataggcaaca tcaccacgcc gacaaacaac
    15301 gagaagagaa tgacgccagc cgccaggaac accaactcat agcgcgcagg gaagacgtta
    15361 ccatccggca agagaagcgg gatagagagc acaccggcca gagtgatcgc cccacgcacc
    15421 ccggcgaaag acgcgatcag gatttctcgt gtagtccacg aaccaaactc catcggcttc
    15481 ttcttcagga agcggttgct gaactttttc atcgtccaca gccagccgaa acggaccagc
    15541 atcagtgccg catatatcag aataatatcg gtaaacagca tccagatttc gacgttaggg
    15601 tcgatttctg ccgccatcag cgacgtctcc agaatccccg gcaactgcag acctaacagc
    15661 aggaacacca tgccgttaaa gacaaattcc agcatcgccc aggtgctgtt tgcgcgcagg
    15721 cgcattgcca gcggcgcacg gcgcatcaca ccggagcggg tgatggtcat cccggcggca
    15781 accgcagcga ggatgccgga aacgccaata tgttccgcaa tcagataaga agcgaatggc
    15841 agcaagaaca gcagaacgat ctgcgtcgcg ggttcatcac cgccccagcg gctgaggaat
    15901 cgcagcgagc gaccatacag ccagctcacc acaaaaccgg cgagaatacc gcctattgcc
    15961 actttcataa attcgaccgt cgcaccgccg acggtgaaga tcattgtccc catcgccact
    16021 gccacggcaa acttcaacga caccaggccg gaggcgtcat tcatcaacgc ttcgccctgc
    16081 aaaatgccca tgattttttt cgggatgcgc ccctcgccaa caatcccgga gagcgctaca
    16141 gcatcggtcg gagaaagcac tgcagccagc gcaaaggcgg ggatcagcgg aatgcccggc
    16201 accacccagt aaataaggaa gccaatgccg accacggtga ccaccaccag cgccagcgcg
    16261 aggccgaaaa tctctcgccc atgttcgaga aattcacggg tcggcgtttt ccagccatca
    16321 gcgaacagca acggcgggat aaacaagact aaaaagagtt caggatcaaa ctccacatgc
    16381 aaaccaaacg tcggccacgc cagtagcgca ccgatggcga tttgcataag cggaagcggg
    16441 atctgaaagg gcatgacacg agtgaccacc ccggacagcg agaccacgag ggtcattatc
    16501 agtatggtga agaagatttc catgcgttcc ctgtttgcga tgttttttat gatctgagaa
    16561 aggcgttatg cctgaattct cattttttca gaatatcgca aaagctcaca caatgtgcgg
    16621 agaaaataaa aacgggcagc cattgccacc cgttttcaga atcctatgta agattagata
    16681 gcccagccac ccgcatagaa ggtcaccagc gccacggcaa taacgaccgt accgatgttc
    16741 aacttgcgcc attcgccgga aaccagacga ccaatcacca gagtcgcgaa gccaatcatg
    16801 atgcctgtta cgatgttaca ggtcagcacg atgaataccg ccgtaaccag acccgccatc
    16861 gcatcaacaa aatcagcaaa gtcgattttc gccacgttgc tcagcatcag caggccgacg
    16921 tacatcagcg ccggagccgt tgcgtacccc ggaacgaggt aagagagcgg agagaggaac
    16981 agaatcagca ggaacagcac gccaacagtg atagccgtca aaccggtttt accgcccgcc
    17041 gccgtacccg ccgcagactc gatgtatacc gctgccggag ctgcacccac gaggccagaa
    17101 aaaacgctgc tcatggagtc ggtggtcagt gctttcccgc cgtcgatgat ctgcccatct
    17161 ttatccagca ggttcgcctg accggcgacg gcacggatag ttccggtggc atcaaatact
    17221 gccgtcatca ccagtgccag aacacttggc aggactacag gattcagcgc acccataatg
    17281 tccaggctgc ctatcagtga attgccgttt tcatcgctca atgaaggcat ggcgaaaacg
    17341 ccggagaaat ggacgttagg atcgaagatc aaaccgacaa ttgagatacc gataatggtc
    17401 agcagaatgc caccagggac tttcagtttt tccaggccga taatcactgc cagccccacc
    17461 aacgacatca tcaccggaaa ggtcgtgaaa tcccccagcg cgacgggcag accatcaagt
    17521 gggtttttaa tcaccagacc gacaccatta gcagcaatca gcagcaggaa cagaccgata
    17581 ccaatccccg tgccgtgcgc cacaccgtgc ggcaagttgc gcaaaatcca gctacggata
    17641 cccgtggcag aaattaccgt aaacagcaca cccatcagga acacggcacc cagcgcgaca
    17701 ggtacgctaa tatgttgccc cagcaccaga ctgaatgcgg taaacgccgt cagagagatg
    17761 gcgcaaccaa tcgccaacgg cagattagcc cacagaccca tcacgataga accgagtccg
    17821 gcaaccagac aggttgcaac gaaaactgcc gcaggcggga agcccgcttt acccaacatg
    17881 cctggaacga cgataaccga gtagaccatc gccagaaacg ttgttaaccc ggcaaccact
    17941 tcctgacgga cagtgcttcc acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg
    18001 gtacgcgctg atggcgtaga cataagaaaa catcccctga gagtttaatt ttcgtcaaga
    18061 tgcgtggcgg acaatcccct gccagcgaaa cgttatcttc ctgctccagg tttgcgacaa
    18121 attggtgcgt cgcaaaaaag caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa
    18181 aagcaaacga ttatccggcg atcatagcgg ctttttcaac cgaagtttgc tgcttttttc
    18241 tcttttattg tcttgacgat gaaaaaccag gcaattgatg cgcaaacgtt atcgtcgatg
    18301 cgcaagattg cagcgttatc gagccgtata ccgttcagga ttgcgataac aaatcgtctg
    18361 agatgacaaa aatattgcgg ctaaaaagtg tacttttaca ctggcgaaga aacgccggtc
    18421 acatacaatt aaagcatcaa caccaaccgg aacctccacc acgtgctcga atgaggtgtg
    18481 ttgacgtcgg gggaaaccct cctgtgtacc agcgggatag agagaaagac aaagaccgga
    18541 aaacaaacta aagcgccctt gtggcgcttt agttttgttc atcttccagt aagcgtgcgc
    18601 cggtaccttc ttctcctaag cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa
    18661 ggcagccaca accaatacat ccatccagtt cgtcacgcag cgccactaag gtatgaatgc
    18721 gccgatccaa ctcttctcgc cattgggacg aaagctgttt ccactctttc gcacttaacg
    18781 tatgcccttc tggcaacacg ccaaacgctt cacgaatggt tgcgagcgga atgccaatac
    18841 gctgagcaat tttgataatt gcgacatatc gcaacacatc acgtttatat cgccgctgat
    18901 tgccgctatt acggatactg gtaatcaacc ctttgctttc atagaaatgc agcgccgata
    18961 ccgccacacc gctgcgtttc gccacttcgc cgggggttag cagcgcttta atgcggggta
    19021 atttcttttc cataaatcgc tttacctcaa gttaacttga ggaattatac tccccaacag
    19081 atgaattaac gaactgaaca ctgaaaagag gcagatttat gtcccatcag aaaattattc
    19141 aggatcttat cgcatggatt gacgagcata ttgaccagcc gcttaacatt gatgtcgtcg
    19201 caaaaaaatc tggctattca aagtggtact tgcaacggat gttccgcacg gtgacgcatc
    19261 agacgcttgg cgattacatt cgccagcgcc gtctgttact ggccgccgtt gagttgcgca
    19321 ccaccgagcg tccgattttt gatatcgcaa tggacctggg ttatgtctct cagcagacct
    19381 tctcccgcgt tttccgtcgg cagtttgatc gcactcccag cgattatcgc caccgcctgt
    19441 agttttattg cccgcgcgtt aactcccgcg cgggtaatta ccccatccat tgcataaaca
    19501 cctgcggcgg catcgccttc gcaaaaaacc atccctggca atagcgcacg ccgtgtttgc
    19561 gcagccagtt aacctgcgcc tcagtttcga caccttcagc gatcgttttt aatcccaggc
    19621 tgtgcgccag ctcgatgatg tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg
    19681 tctcaacaaa cgatttatcg attttcaaaa tatcgacatt caatgattta aggttatgca
    19741 agttagagta gccaatacca aaatcatcaa ttgccacttc gtaaccggcc tggcggaaag
    19801 ccagaataat cggcgtcatt ttgtcgacat caagaaatgc atgttcagtc acttcaaatt
    19861 taatttgctg cggacgcacc gcatgttgct ctgttttctg attggttcgc gctatcaacc
    19921 gtgaggtatg aaaatcggag gctgacaggt taatagaaac atagcgatcg gtatgtgttg
    19981 ccagatacgc gcccaaatcg cgaaagacat tatcaataac ataatcagtt acctgttcga
    20041 tcatcccctc cttttctgcc agcggaataa actctgccgg attcattact tgcccctgct
    20101 caccaggcca acgtaacaac gcttcagcac cgatacattt ttctgttttg atatcgatta
    20161 ttggctggta ataaaggcaa agctgatgtt tttcgagggc gcgttgcaat ttgcgtttgg
    20221 gagacaaata gttttgtcga atacgtagcc agagtaatag cagaacaaga ctccccaaaa
    20281 taccggcggg taacgcaaaa ataagatgat tataaaaatg ggttataaga cgttgatatg
    20341 aagtcgcaac aatggctgca attgggcgtt ttgtcgaata aactgtcgca tataaatagc
    20401 catttcgttg tacggttaaa tcattcaaat gaatcagcgg agaaaacgtt gctgccgagg
    20461 cctcgttgct taacgagaaa aaggttttcg tcaccgtatc atacacaccc cattgcaatg
    20521 tcggatcatc agacatcact tcactccaga agagagggtt gataaccgcc acataatttc
    20581 cccgctgcat gtaggtcatt ttatagccag agaaaaaagg cgtatcacgg taataataga
    20641 tagaaacgtt aggttcacgc ttataatcgg ccggtgcaat cgtatagcca tttacagacg
    20701 ctatcagcga tgagcataaa aaatggttat cacgagcata aatcaattca ttaatataaa
    20761 gatagccacg aataatattc aacattcgct tttgatgggc tggagtgcaa acttgcccct
    20821 gatagcgttc agctgcatcg ctcactaaat ctgcctgacg aatgaccagc tcagatttat
    20881 ccaacgccag ctgagcaaag gttcggagct gattattcac ttctgatttt gcccaaaaga
    20941 aggcaatcca tagcgacaga atgatgggaa agagaactaa aaagataatg cccggaaacg
    21001 ccagtaattg gtgtcgtgca cgatgactca ttgctatcaa ttccctgcct gtttataccc
    21061 ggttattgca aggtgaaacc ctggctaccg acgtctttag ataaatctta cggtataaat
    21121 agcacttttg cctgatataa aattgagacc tttttaatcg tcaccaggat gacgataatt
    21181 cattgatctt aaaaattaat caccttcagg aatgcagagg cggcgggaag atataccctt
    21241 aatgtcgctg aaaaaacaca taatctgtac ggcaataaaa gtcacggcct aatctggcta
    21301 acaaacaacc aattgtgata tagttcacaa aattcatgaa acaaacagag tgtttcattt
    21361 ttgtgttctg caagccccgc gatagccaga gtgtatcacg cctcccgtga acaacgccgc
    21421 gctgtccagg gcatcagctc ttttccgagg aattgtttga tggctaccct caccactggc
    21481 gtggttcttc ttcgctggca acttcttagt gccgtaatga tgtttctggc tagcacgctc
    21541 aacatccgtt ttcgtcgggc tgattatgtc gggcttgcag tgatcagcag cggtctgggc
    21601 gtagtttccg cctgctggtt cgcaatgggg ttgcttggca tcacaatggc ggatatcacc
    21661 gccatctggc acaacatcga gtcggtgatg atagaagaga tgaatcagac accgccacaa
    21721 tggccaatga ttttgacttg atatgtagaa gcctccataa cggaggcttc ttttttatgg
    21781 ctggcagatg ctttaatcat ccaccttaaa acaatataac ctattgtttt aaagacaaat
    21841 cagaacggaa tatcgtcatc aaagtccatc ggcggctcgt tagacggcgc tgccggagcg
    21901 gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccaccctg cggctgctga
    21961 ggctgacccc aaccgccctg cggctgacca ccaccgatat tgccacctgc aggagcgccc
    22021 cccccctgac gaccacccag catctgcatg gtgccgccaa cgttcaccac gacttctgtg
    22081 gtgtagcgat cctgaccgga ttgatcggtc catttgcggg tacgcagctg accttcaata
    22141 taaacctgag aacctttacg cagatattcg ctggctactt ctgccagttt gccgaacagc
    22201 acaacgcggt gccattcagt ctgctctttc atctcgccgg tcgctttatc acgccaggat
    22261 tcggaagtag ccagcgtaat gttggcaact gcgccaccat ttggcatgta gcgtacttcc
    22321 gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct ggccatgttc
    22381 gtgtctcctg aaaaaaaatc gttctgaata agtgtaaacg cgcgattgta ccattaccaa
    22441 tagcgctttt actatgttgt gacctcggtt ccgggaaaca aacctggcca gacattgtta
    22501 cacaacactc cgggtaatgc attccaatac tgtatattca ttcaggtcaa tttgtgtcat
    22561 aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg
    22621 ggaaaggtga atggataaga tcgaagttcg gggcgcccgc acccataatc tcaaaaacat
    22681 caacctcgtt atcccccgcg acaagcttat tgtcgtgacc gggctttcgg gttctggcaa
    22741 atcctcgctc gctttcgaca ccttatatgc cgaagggcag cgccgttacg ttgaatccct
    22801 ttccgcctac gcgcggcagt ttctgtcact gatggagaag ccggacgtcg accatattga
    22861 agggctttct cctgccatct caattgagca gaaatcgacg tctcataacc cgcgctctac
    22921 ggtggggaca atcaccgaaa tccacgacta tttgcgtctg ctgtacgctc gcgttggtga
    22981 gccgcgctgc ccggaccacg atgtaccgct ggcggcgcaa accgtcagtc agatggtgga
    23041 taacgtgttg tcccagccgg aaggcaagcg tctgatgctg ctcgcgccaa tcattaaaga
    23101 gcgcaaaggc gaacacacca aaacgctgga gaacctggca agccagggtt acatccgtgc
    23161 tcgtattgat ggcgaagtct gcgatctttc cgatccgccg aaactggaat tgcaaaagaa
    23221 acataccatt gaagtggtgg ttgatcgctt caaagtgcgt gacgatctta cccaacgtct
    23281 tgccgagtcg tttgaaaccg cgctggagct ttccggtggt accgcggtag tggcggatat
    23341 ggacgacccg aaagcggaag agctgctgtt ctccgccaac tttgcctgcc cgatttgcgg
    23401 ttacagtatg cgcgagctgg aacctcgcct gttttcattt aacaacccgg cgggagcctg
    23461 cccgacctgt gacggtcttg gcgtacagca atatttcgat cctgaccgcg tgatccagaa
    23521 tccggaattg tcgctggctg gcggtgcgat ccgtggctgg gatcgccgca acttctatta
    23581 tttccagatg ctgaaatcgc tggcagatca ctataagttc gacgtcgaag cgccgtgggg
    23641 cagcctgagc gcgaacgtgc ataaagtggt gttgtacggt tctggcaaag aaaacattga
    23701 attcaaatac atgaacgatc gtggcgatac ctccattcgt cgtcatccgt tcgaaggcgt
    23761 gctgcacaat atggagcgcc gttataaaga gacggaatcc agcgcggtac gtgaagaatt
    23821 agccaagttt atcagcaatc gtccgtgcgc cagctgcgaa ggaacccgtc tgcgtcggga
    23881 agcacgccac gtgtatgtcg agaatacgcc gctgcccgcc atctccgaca tgagcatcgg
    23941 tcatgcgatg gaattcttca acaatctcaa actcgcaggt cagcgggcga agattgcaga
    24001 aaaaatcctt aaagagatcg gcgatcgtct gaaattcctc gttaacgtcg gcctgaatta
    24061 cctgacactt tcccgctcag cagagacact ttccggcggt gaagcccagc gtattcgtct
    24121 ggcgagccag attggtgcag gcctggttgg cgtgatgtac gtgctggatg agccgtctat
    24181 cggcctgcac cagcgcgata acgagcgcct gctgggtacg cttatccatc tgcgcgatct
    24241 cggtaatacc gtgattgtgg tggagcacga cgaagacgcg attcgcgccg ctgatcatgt
    24301 gatcgatatc ggtccgggtg cgggtgttca cggcggtgaa gtggtcgcgg aaggtccact
    24361 ggaagcgatt atggcggtgc ctgagtcgtt gaccgggcag tacatgagcg gtaaacgcaa
    24421 gattgaggtg ccgaagaaac gcgtacccgc caatccagaa aaagtgctga aactgacggg
    24481 cgcacgcggc aacaacctga aagacgtgac gctgacgctg ccggtaggtc tgtttacctg
    24541 catcacaggg gtttcaggtt ccggtaaatc gacgctgatt aacgacacac tgttcccgat
    24601 tgcccaacgc cagttgaatg gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca
    24661 gggactggag catttcgata aagtgatcga tatcgaccaa agcccaattg gtcgtactcc
    24721 gcgttctaac ccggcgacct ataccggcgt gtttacacct gtgcgcgaac tgtttgcggg
    24781 cgtaccggaa tcccgtgcgc gcggctatac gccaggacgt ttcagcttta acgtccgtgg
    24841 cggacgctgc gaggcctgtc agggcgacgg cgtgatcaaa gtggagatgc acttcctgcc
    24901 ggacatttac gtaccgtgcg accagtgcaa aggcaaacgc tataaccgtg aaacgctgga
    24961 gattaagtac aaaggcaaaa ccatccacga agtgctggat atgaccatcg aagaggcgcg
    25021 tgagttcttt gatgcggtgc cagctctggc gcgtaagctg caaacgttga tggacgttgg
    25081 cctgacgtac attcgcctcg ggcagtccgc aaccacactt tctggtggtg aagcccagcg
    25141 cgtgaagctg gcgcgtgagc tgtcaaaacg cggcaccggg cagacgctgt atattctcga
    25201 cgagccgacc accggtctgc acttcgccga tattcagcaa ctgctcgacg tactgcataa
    25261 actgcgcgat cagggcaata ccattgtggt aattgagcac aatctcgacg tgatcaaaac
    25321 cgctgactgg attgtcgacc tgggaccgga aggcggcagt gggggcggcg aaatcctcgt
    25381 ctccggtacg ccagaaaccg tcgcagagtg cgaagcttcg catacggcgc gcttcctcaa
    25441 gccgatgctg taatcgttaa ggccgctttc agagcggcct tcttctttca gagttgcacc
    25501 agcaatttac gtttttcttc cggcagcaaa ttcaccgcct gctgataaga cgcatccacc
    25561 agataataga tttgcgaatc cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct
    25621 ttattcagat ggcggcttgg acgcacatcg ctgtgctgct gacgtagcag ctccgccagc
    25681 tccgggctgg ttttcagcga aacagctggg cgattttcta cttctttcac catcgcaaac
    25741 agcacatctt ccactttgat ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg
    25801 cctggttttg ccatgcaata ttgtagcaac tccgaaatgg tcatttttca ctccccttgt
    25861 agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat gttcccccag ccagatgcct
    25921 tgccaggtgc cggtctgaat acgcccttta tgcaccggca atacaagcga tgttcccagc
    25981 attgaggatt tgatatgaga aggcatatcg tctgctccct catagtcatg ctcataattt
    26041 ccgttatcgg gaacggcgcg gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg
    26101 cagttctcat taagtgtcag agaggcggag gtatgttgca gcaacagatg cagtaaaccg
    26161 atgttaacgc gcggcatatc agccagctga ttcagaattt catccgttac cagatgaaac
    26221 ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc acatgtgctg ctccttgata
    26281 aaactctctt aatcagtttg cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac
    26341 agcattcggg agagtattac gctctcccgc tctgtcagta ttctgaattg acgatcacct
    26401 cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa
    26461 tgcggatacc acgagcgcca acatcgcgtg cggcggtaat atcgttatcg gaatcaccat
    26521 aaaaaattcg gatattttta tcctgcagcc attgcgattt tgtattttga cctggcttat
    26581 cgcccgcaaa gatcaccgga ttcatgttgg tggcaggaat atgaaaatta tccgccagcg
    26641 tttttgaaac cgtttctgtt ttggtcgggc tacgaccagt cacaaagaag atcgcgtcac
    26701 cgcggcgtac atgcatatca atcagctggc gagcgacctc ttttggaatg ctgaattcat
    26761 cccagccatt gttcattttt tcccagaaca caggattttt cagataatct tcgctttctg
    26821 gcgagaaggt ttttttgccg cgccagaaac ccggactgga aaaaagtacc gtatcatcga
    26881 tatcaaaccc caccgccatt ggcggacgtc ctgcgaggct attttcaatt tgtgcaaccg
    26941 aaacccaatg aattggcgcc tgttcagcga gtttggctac gttagtaccc ggattaagcg
    27001 gtgaaggaga tgaggccagg gcaacagcgg aactgtttag agcgaacaat aagcaaacgg
    27061 cactcaatgc ctgtgtgatc ttgcgcatat ttttccctaa atagtcagta agttgaaact
    27121 tattattgtg cgtttgttga aaaaaccatc tgaccataac gccaaccgcg ggggaaagga
    27181 aggattatct gcggatttgt cacataaaaa gagcatgatc acatttggaa tacctgtgcg
    27241 ataaaccaca cattgccacg gttattctgc ttacacattc acacaatggc actcttttgg
    27301 ttctcgcaaa cattgttcag ggaatatact gatgataaat ataaatgcat tcaatggatg
    27361 atgaatgaaa atatcgtgga attatatatt taagaacaaa tggcgatttc acattacaag
    27421 catttcactt tttcttatca tgctcgcggt ttcaatcgct tttttgcact tgcgttttaa
    27481 taccttgtcc ggtaccgata aaatgcggct tgaaatgtat aagtccacat tatattccac
    27541 catcgagcaa ttttatgttt taccctatat gctctcaaca gaccatatca tccgtcaggc
    27601 ggtaattacg cctgacgata tgacgtccag cgaactcaat caacgaattg cacatttcaa
    27661 tactcaactc aaaaccgcag caatatttat tctggatacc caaggtaagg ccatcgcttc
    27721 tagcaactgg caggaccccg gcagctatgt agggcaaaat tatagctatc gcccctatta
    27781 taaacacgcc atgtctggct taaatggacg cttttacggt attggtagca ctacaaatac
    27841 accgggattc ttcctctcta caagtataaa agataaagga aaaattgtcg gtgttgtagt
    27901 agtaaaaata agtcttaatg aaattgaaaa agcatggtcc gaaggtcctg aaaacattat
    27961 cgttaatgat gaacatggga ttatattttt aagttcaaaa tcgccatggc gaatgagaac
    28021 gctgcaaccg ttacctgttc aggcaaaaca aaaacttcaa tctacccgtc aatatagcct
    28081 ggacaatctt ttacctgcgg attattaccc ttgttttacc gttagcaatt ttactttcct
    28141 gaaagataaa aaagaacaat tctgcttatt cccgcagtat tatacgcaac aaatcgccat
    28201 tccagagttt aactggaaaa tgacaattat ggtcccctta gataacctgt actggtcatg
    28261 ggctatttcg ttagtcatta cactaattat ttacctgctg tttttgttat ttattaaata
    28321 ctggagaatg cgatctcatg cacaacaatt attaacactg gcgaatgaaa cattagaaaa
    28381 acaggttaaa gagcgtacat ctgccctgga attgatcaat caaaaattaa tacaggagat
    28441 aaaagagcgc agtcaggctg aacaagtatt acaaattacg cgtagtgaac tggcagagtc
    28501 cagcaaactg gcggcgcttg gacagatggc aaccgaaatt gcccatgaac aaaatcaacc
    28561 gttagccgcc attcacgcac ttactgataa cgcgcgtact atgctaaaaa aagagatgta
    28621 tccgcaggtt gaacagaatc tgaaacacat tatttcagtg attgagcgga tgacgcagct
    28681 catttccgaa cttaaagcat ttgcctcgcg ccatcgcgta cctaaaggtt ctgccgatgt
    28741 catcaaagtg atgtatagcg ccgtggcgtt acttaatcac agcatggaga aaaataacat
    28801 tgagcgacga ataaaagccc catccatgcc gttatttgtc aattgcgatg agctcggtct
    28861 tgaacagata ttcagtaatt taattagcaa cgccttagat tctatggaag gtagctctta
    28921 caaacgactg gatatcgcca ttcgccaggc aaataacaaa gttattatta ccattaaaga
    28981 cagcggtggc ggttttgcac ctgaagttgt cgatcgcata tttgaaccat tttttaccac
    29041 taaacgtaga ggaatggggt tgggactggc aatagtcagc gaaattgtcc gaaattcgaa
    29101 cggcgcactc cacgccagta atcatcctga aggcggcgca gtaatgacat taacctggcc
    29161 tgaatgggga gaagaacatg aataataaat ccagtgaaat tactatagtt tatattgaag
    29221 atagtgacga tgtacgtttt gcctgtgaac aaacgctcac gctggctggt tatcgtgtta
    29281 tttcctgttg cgatgcagaa cacagtattc cccttattca aagtcaggcc aacattatta
    29341 tactgaccga tgtcaggtta cctggaatat caggactaga gctcctcagt tacatcaacg
    29401 aaatggatag caaaattccg gttattttaa ttaccggaca cggtgacgtg gaaatggcgg
    29461 tggatgccat gcgtaatggt gcattcgatt tcattgagaa accaagtagc tcagataaat
    29521 tattgagtat tattgccaga gcggtagaaa aacggcgatt ggttttagaa aatcagcaat
    29581 tactggcgaa tctgcaacag gaaaatggcc cggtgctgat tggtcgtagt ccgcagatgc
    29641 aacaactgcg taaaatgatc cttaatgtcg cagataccgg tgccgatgtc cttatttatg
    29701 gtgaaacagg ttgtggtaaa gaagtcgtcg ccagaatgct gcatcactgg agtacccgcc
    29761 gtcaggggca gtttgttgcc ttaaactgtg caggcttacc agaaacgttg tttgaaagcg
    29821 aaatatttgg tcatgaggcg ggcgcattta ccggtgcggt taaaaagcgt attggaaaaa
    29881 ttgaacatgc aaatggtggt acgttatttc tcgatgaaat agagggaatg ccatcaggaa
    29941 tgcaggtaaa actgctacgt gtattgcagg aaagaaccat tgaacgatta ggtgccaatc
    30001 aattaattcc ggtgaattgc cgtgttattg cagcgaccaa ggaagattta ctgcgtcgca
    30061 gcgaagaaca tctgtttcgt ctcgatctct attatcgtct gaatgtggtt agcctgaata
    30121 ttccacctct acgccagcga cgagaagata tccctgaact gttttactgg ttcgccagtc
    30181 aggcggcaca aaaatataat cgtcctctgc ctgatatttc tcccatgtta ctggcctggt
    30241 tgcagtcaca aagttggcct ggcaatgtac gtgaacttaa acacaatgca gagcgttttg
    30301 ttctcggttt actgacccac caccagccag tgccaatgac tcagcaagaa gagagtggtc
    30361 ttaccgcctg cattgatgct ttcgagaaaa agttaatcga agatatgctg cgacaaacgg
    30421 aaggccaggt cagtttgaca gcgcggctat tacagctgcc gcgcaagact ttatatgaca
    30481 aactcaacaa acatcagatt caaccgcaag tttatcgacc agaatgatac cgggatcgta
    30541 atcatcttcg cattgcgttc ccggttggca gcttctggca taccggggta aatcaccgga
    30601 ttgccgccag gttgaggctg cgtagctaac agataatccg caaagaactg ccctttctcc
    30661 cctgccagat cggtgaattg atcaggatcg ataatcaacg caaaaagatg attatgcgca
    30721 gatccatccg tctgatgatc ttcggcgatc gtgtcaccac cagataagat ccccgccatc
    30781 atttcaatca tcagtgcaag gccaaatcct ttatgctcac caaatggcag taacgcccct
    30841 ggaggattgc tgaataacac cccaggatca ttgcttggat tcccctgtgc atcaatcaat
    30901 tgtcccggcg cggcctgctt cccggtggac gacattaccc gtgctttatt tagcgccatg
    30961 ctactggtgg caaaatcaac cagaatcggc ggacgcccat cactgaacgg ccaggcaaag
    31021 cagaaagggt tagtggtcat acgcggttcc gcaccgcccc acggggcgac cagtgcagga
    31081 cgcccggtga cgttactcat ggccagcatc gccaggcctt gatcagcaac catttgcccg
    31141 taatgcccca tacgccctaa atgatgcgca tggcacaacg ttaacaggca aaaaccgcgg
    31201 tgttttgctt gttcgatggc agcatttacc gccacttttc cggcgtgttg accaaagcca
    31261 ttgtgggcat gaaaacgttg taaattacct tcacttgtta agcattccgg gcgggcattc
    31321 gcggttacat cgccacgagc gatattttcc agatatttcg gtagcagcgt gacgccgtgg
    31381 ctggcaaagc cataacgttc cgcttcaatc atatgttccg ccacatcctg agcgatatcg
    31441 gcatcacagc cattaagttc cagcagcccg gcagctaacc aacgcacttt ttcaacagca
    31501 atttgtaatg acatcgttgg tttcctcaca ttagacctaa aattttccac caggtggcgg
    31561 caaacagtag caacaacagg tagccgatca ccgtcaggta cagtcccaca cgggtaaact
    31621 ggcgtggcgt gaaggtatcc gtacccatac acaccatatt ttgtggcgcg ttaatcggca
    31681 ggatgaagcc aaaactgaca ctgaaggcga gcagaatggt catgccgacc gggttaacgc
    31741 cgagttctgg cggcagactg cttaacaggc taatcaggat gggcagcaat gcggcagtta
    31801 gcgcagtggc actggcaaac cccaggtgaa taattatcaa gaaagcggcg agaatcgcga
    31861 agatggctaa tgatggcaag ccatcgagac caaaaccttt caccacatag ttcgccatcc
    31921 atgaagctgc ctgcgtatca agcagcgtgg accccaggct gataccaata ccaaacataa
    31981 gcaacgtgcc ccactgcaca cgtttttcga cctctttcca gctcattaca ccgatgcctg
    32041 gcagcaacat aatcgccagt ccggcaaggg tgacagaggt ggtatcaata ctgtgtaatt
    32101 ttccgccagt agaccaaaag agcagtaaca atagcgaaat accgatcaaa cgtttttctt
    32161 taccagtggt tggccctaac tctgcaagtg cttttttaat ggcctcgctc cccccttcaa
    32221 cagcttccgt ttctggcggc aacaatttac gtgcgaggaa atagaggatc gccgacatgg
    32281 tgaggctcca gggcgcgccc gctaacagcc agtcaagcca gctgacagaa tgcccggcgc
    32341 cgaaagtttt attgataaaa ccgatagaaa gcaggttttg cgccgccgaa gtttgaatac
    32401 caacgttcca gatactggtc gcctgggcga taacaatcat cattgacgcc gctaaacgtg
    32461 aatgtttatc caccttaaat gccgcaatga cccccatcat aatcggcacc acgcaagccg
    32521 tacgggcagt ggcactgggt acaaccaggc tcaggacgat ggtcaccaca atggcaccga
    32581 taataatgct gcggctgctg gcacctattt tcgacatagt aaacagcgca atgcgtttat
    32641 ccagcccggt aatggtcatt gccgccgcga taaacattgc tgctgccacc agcgccagag
    32701 cagaattaga aaaacccgac agagtcattt tcagcgcttt cgcggtgcca aggatcgtgt
    32761 ccgggtgatt catatccggg gcaaaaccaa ccataaagat aatcagtgcg gaaataacaa
    32821 ttgcactggc ggtgtaatcc atcgcttcgc tgatccagac aataatggca aacaccagaa
    32881 tggcgatcat attttgtcca gcgacgggca aggtatcgcc cagtggcaga agggtaataa
    32941 taattccggc aatgatcgcc aaccacaggc cgggattagg gataattcga cgcttttgtg
    33001 gtgcgccatg ggatattgat gacatattca cctcattgtg
//
LOCUS       DADTFG010000039        32920 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.039, whole genome shotgun sequence.
ACCESSION   DADTFG010000039 DADTFG010000000
VERSION     DADTFG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.039"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            30..395
                     /locus_tag="J1138_001703"
     CDS             30..395
                     /locus_tag="J1138_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBA8453512.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            544..1062
                     /locus_tag="J1138_001704"
     CDS             544..1062
                     /locus_tag="J1138_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBA8453513.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1052..2278
                     /gene="dgcN"
                     /locus_tag="J1138_001705"
     CDS             1052..2278
                     /gene="dgcN"
                     /locus_tag="J1138_001705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBA8453514.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2294..2776
                     /locus_tag="J1138_001706"
     CDS             2294..2776
                     /locus_tag="J1138_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBA8453515.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2853..3200)
                     /gene="rplS"
                     /locus_tag="J1138_001707"
     CDS             complement(2853..3200)
                     /gene="rplS"
                     /locus_tag="J1138_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBA8453516.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3242..4009)
                     /gene="trmD"
                     /locus_tag="J1138_001708"
     CDS             complement(3242..4009)
                     /gene="trmD"
                     /locus_tag="J1138_001708"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBA8453517.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4040..4588)
                     /gene="rimM"
                     /locus_tag="J1138_001709"
     CDS             complement(4040..4588)
                     /gene="rimM"
                     /locus_tag="J1138_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBA8453518.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4607..4855)
                     /gene="rpsP"
                     /locus_tag="J1138_001710"
     CDS             complement(4607..4855)
                     /gene="rpsP"
                     /locus_tag="J1138_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBA8453519.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4992..6353)
                     /gene="ffh"
                     /locus_tag="J1138_001711"
     CDS             complement(4992..6353)
                     /gene="ffh"
                     /locus_tag="J1138_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBA8453520.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6445..7311
                     /locus_tag="J1138_001712"
     CDS             6445..7311
                     /locus_tag="J1138_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBA8453521.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIFQQLIS"
     gene            7332..8618
                     /locus_tag="J1138_001713"
     CDS             7332..8618
                     /locus_tag="J1138_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBA8453522.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQVLMMPLVWLLNAITRILMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8673..9266)
                     /gene="grpE"
                     /locus_tag="J1138_001714"
     CDS             complement(8673..9266)
                     /gene="grpE"
                     /locus_tag="J1138_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBA8453523.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9389..10267
                     /gene="nadK"
                     /locus_tag="J1138_001715"
     CDS             9389..10267
                     /gene="nadK"
                     /locus_tag="J1138_001715"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBA8453524.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10353..12014
                     /gene="recN"
                     /locus_tag="J1138_001716"
     CDS             10353..12014
                     /gene="recN"
                     /locus_tag="J1138_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBA8453525.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQTGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12163..12504
                     /gene="bamE"
                     /locus_tag="J1138_001717"
     CDS             12163..12504
                     /gene="bamE"
                     /locus_tag="J1138_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBA8453526.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12566..12856)
                     /locus_tag="J1138_001718"
     CDS             complement(12566..12856)
                     /locus_tag="J1138_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBA8453527.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12846..13322)
                     /gene="ratA"
                     /locus_tag="J1138_001719"
     CDS             complement(12846..13322)
                     /gene="ratA"
                     /locus_tag="J1138_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBA8453528.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGG
                     WKFTPLSQDACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13454..13936
                     /gene="smpB"
                     /locus_tag="J1138_001720"
     CDS             13454..13936
                     /gene="smpB"
                     /locus_tag="J1138_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBA8453529.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14151..14513
                     /gene="ssrA"
                     /locus_tag="J1138_001721"
     tmRNA           14151..14513
                     /gene="ssrA"
                     /locus_tag="J1138_001721"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            15196..16173
                     /gene="csiD"
                     /locus_tag="J1138_001722"
     CDS             15196..16173
                     /gene="csiD"
                     /locus_tag="J1138_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBA8453530.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTNR
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAMGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLHRELMRQRGYFAYATHHYQTHQ"
     gene            16193..17461
                     /gene="lhgO"
                     /locus_tag="J1138_001723"
     CDS             16193..17461
                     /gene="lhgO"
                     /locus_tag="J1138_001723"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBA8453531.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCEQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIDREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVICTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEIKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFYQ"
     gene            17484..18932
                     /gene="gabD"
                     /locus_tag="J1138_001724"
     CDS             17484..18932
                     /gene="gabD"
                     /locus_tag="J1138_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBA8453532.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPTGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQNGVYDRFAEKLQQAVSKLHIGNGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            18946..20226
                     /gene="gabT"
                     /locus_tag="J1138_001725"
     CDS             18946..20226
                     /gene="gabT"
                     /locus_tag="J1138_001725"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBA8453533.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            20463..21863
                     /gene="gabP"
                     /locus_tag="J1138_001726"
     CDS             20463..21863
                     /gene="gabP"
                     /locus_tag="J1138_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBA8453534.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            21884..22546
                     /gene="csiR"
                     /locus_tag="J1138_001727"
     CDS             21884..22546
                     /gene="csiR"
                     /locus_tag="J1138_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBA8453535.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVTGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTATVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(22547..22996)
                     /gene="kbp"
                     /locus_tag="J1138_001728"
     CDS             complement(22547..22996)
                     /gene="kbp"
                     /locus_tag="J1138_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBA8453536.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(23080..23238)
                     /locus_tag="J1138_001729"
     CDS             complement(23080..23238)
                     /locus_tag="J1138_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBA8453537.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            23421..23720
                     /locus_tag="J1138_001730"
     CDS             23421..23720
                     /locus_tag="J1138_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8453538.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            23730..24254
                     /gene="ygaP"
                     /locus_tag="J1138_001731"
     CDS             23730..24254
                     /gene="ygaP"
                     /locus_tag="J1138_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBA8453539.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQSLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(24301..24705)
                     /gene="stpA"
                     /locus_tag="J1138_001732"
     CDS             complement(24301..24705)
                     /gene="stpA"
                     /locus_tag="J1138_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBA8453540.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            25372..25821
                     /gene="alaE"
                     /locus_tag="J1138_001733"
     CDS             25372..25821
                     /gene="alaE"
                     /locus_tag="J1138_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBA8453541.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(25858..26202)
                     /locus_tag="J1138_001734"
     CDS             complement(25858..26202)
                     /locus_tag="J1138_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBA8453542.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            26354..26683
                     /locus_tag="J1138_001735"
     CDS             26354..26683
                     /locus_tag="J1138_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBA8453543.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(26740..28074)
                     /locus_tag="J1138_001736"
     CDS             complement(26740..28074)
                     /locus_tag="J1138_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA8453544.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITSGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEGMARQVAQQLRKALS"
     gene            28162..28593
                     /locus_tag="J1138_001737"
     CDS             28162..28593
                     /locus_tag="J1138_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBA8453545.1"
                     /translation="MTTLRQPYYELSPAVYNALVQATTALENSTLDTTLMELVYLRIS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            28801..29046
                     /gene="nrdH"
                     /locus_tag="J1138_001738"
     CDS             28801..29046
                     /gene="nrdH"
                     /locus_tag="J1138_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBA8453546.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPRAASA"
     gene            29043..29453
                     /gene="nrdI"
                     /locus_tag="J1138_001739"
     CDS             29043..29453
                     /gene="nrdI"
                     /locus_tag="J1138_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBA8453547.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCS
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            29426..31570
                     /gene="nrdE"
                     /locus_tag="J1138_001740"
     CDS             29426..31570
                     /gene="nrdE"
                     /locus_tag="J1138_001740"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBA8453548.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            31580..32539
                     /gene="nrdF"
                     /locus_tag="J1138_001741"
     CDS             31580..32539
                     /gene="nrdF"
                     /locus_tag="J1138_001741"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBA8453549.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLLPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFASRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISLAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         8172 a         8485 c         8130 g         8133 t
ORIGIN      
        1 tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa atttatcgcc cccttgttgg
       61 ctttactggt tagcggatgt cagattgatc cttatactca cgcgccaacc ttgaccagca
      121 ccgactggta tgatgttggt atggaagatg cgatatcggg cagcgccatt aaagatgacg
      181 atgcatttag cgattcacag gcggatcgcg gtctatacct taaaggatat gccgaagggc
      241 aaaagaaaac ttgccagacc gattttactt atgcccgggg actttctggt aaaagctttc
      301 ctgccagctg caataatgtt gaaaatgcca gccaactgca tgaagtatgg cgaaaagggg
      361 ctgatgaaaa tgccagcaca atacgattaa attaattacc cccccaaaat caattaagca
      421 tgagatttta ctcattatgt tttatttctc acattgatga cgaccgcatg aaaggatgat
      481 attttaatga gcattctgaa aaaatatcga tgcatttcga gcgaagatgg tgaggatccc
      541 tgaatgcgtt tttctcaccg actgttctta ctgctgattc tcctgctgac gggagcacca
      601 atactcgctc aggaaccttc tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt
      661 gtcagttaca cccgctggcc tgggctatca gggccaccaa agttatgtat cttttcatct
      721 tcacgtttta gtaccgcctt acaagaaaat gctgctacat ctttacctta tctgcctgtc
      781 atcattcata ctcaacaaga ggcgataatt tcaggctgca atggttttta ttttggcaac
      841 gagtcaccga cattccagat ggaattaacc gaacaatatc cgtcaaaggc gttattatta
      901 attgccgaac aaaataccga gtgcattatt ggtagcgcct tttgtctgat catccacaat
      961 aatgacgtca gatttgccgt aaacctggat gccttatcgc gtagcggtgt aaaggtcaac
     1021 ccggatgtct taatgctcgc acggaagaaa aatgatggat aacgataatt ctcttcataa
     1081 gcgccccacg tttaaaagag cattacgcaa catcagcatg accagcatat ttatcactat
     1141 gacgctgatc tggctgctgc tttccgtgac gtcagtgcta accctgaaac aatacgcgca
     1201 aaaaaacctg gcactgacag cagcaacaat gacttacagt ctggaagcag ctgtcgtttt
     1261 tgccgatggc cctgcagcaa ctgaaacact ggcagcgctg ggccagcaag gccaattttc
     1321 aactgcagaa gtgcgtgata agcagcaaaa tattctggca tcctggcatt acacccataa
     1381 ggaaccaggc gatactttca gcaatttcat aagccactgg ctcttccctg cccccatcat
     1441 tcagccgatt cgtcacaatg gtgaaaccat tggcgaagta cgcttaactg ctcgcgacag
     1501 ttcaatcagc cattttatct ggttttcgct cgctgtcctg accggttgta ttctgctggc
     1561 atcaggcatc gcaattaccc tcacccgcca tttgcacaat ggcctggtgg aagcgctgaa
     1621 aaatatcacc gatgtcgtac atgatgtgcg ttctaaccgc aatttttccc gacgagtttc
     1681 ggaagaacgt atcgctgagt ttcaccgctt cgctctcgac ttcaacagtc tgctggatga
     1741 aatggaagag tggcagcttc gtttacaggc caaaaatgcg cagcttctac gtaccgcgct
     1801 acatgaccca ttaaccgggc tggctaaccg cgcagcgttt cgtagcggca tcaacacgtt
     1861 gatgaacaat tccgatgccc gaaaaacgtc ggcgttacta tttcttgatg gcgataattt
     1921 caaatatatc aatgatacct ggggtcatgc gacgggcgat agagtcttga ttgaaatagc
     1981 aaaacggtta gctgaatttg gcgggctgcg acataaagca taccgcctgg gcggcgatga
     2041 attcgctatg gtgctctatg atgtacagtc ggaatctgaa gtgcagcaga tatgctcagc
     2101 actgacacaa atttttaatc tcccgtttga tcttcataat ggccatcaga ctaccatgac
     2161 attaagcatt ggttacgcga tgaccattga gcacgcctct gcggaaaatt tacaagagct
     2221 tgccgatcac aatatgtatc aggccaaaca ccagcgtgcc gaaaagctgg tgagataaca
     2281 aggatatatc gatatgataa agcacctgct agcacccctg attttcacct cgctaatact
     2341 tactggctgc cagtcccctc agggaaagtt tactcctgag caagtcgccg ctatgcaatc
     2401 ttatggattt actgaatccg ccggcgactg gtcgctgggc ttatcagatg ccattctgtt
     2461 tgcaaaaaat gactacaaat tgctcccgga aagccagcaa cagatccaaa ccatggcagc
     2521 caaattggcc tcgacagggc taacacatgc ccgtatggat ggacacaccg acaactatgg
     2581 tgaagacagt tacaacgaag tcttatcatt gaaacgggcg aatgtcgtgg ccgatgcatg
     2641 ggctatgggt ggacaaattc cgcgcagcaa tctcaccact cagggtttag gaaaaaaata
     2701 tcccatagcc agtaacaaga ccgcccaggg ccgcgccgag aaccgccgcg tcgcagtggt
     2761 gattacaacc ccttaaaaca cgagcaaaca gacaaaaaaa gggccagcca attggccagc
     2821 ccttcttaac aggatgtcgc ttaagcgaaa tcttagttaa gacgctcttt gatacgagca
     2881 gccttaccag tacgctcacg caggtagtac agtttagctt tacgaacagc accacgacgt
     2941 ttgacagaaa tgctgtcaac taccggagag tgagtctgga agacacgctc aacgccttcg
     3001 ccgttggaaa ttttacgaac agtgaatgca gagtgcagac cgcggttacg aatagcgata
     3061 accacgccct cgaatgcctg cagacgtttt ttggaacctt caacaaccca tactttcact
     3121 tccacggtat cacccggacg gaaggaaggt acgtcctgct tcatctgctc ttgttcaagt
     3181 tgcttaataa tgttgctcat aatttaatct cttatcctgg gtaaactgat atctcggggg
     3241 cttacgccat cccatcatgt ttatgttgct gttgtgcgtg ttccgttttg aactccgcca
     3301 gcaaccttgc ttgctcttca gtcagagcca ggttttccag aagttcaggt cttctaagcc
     3361 aggtacggcc cagcgactgt ttcaaacgcc agcgacgtat ctcggcatgg ttgcccgaca
     3421 gtaacactgg cggaacttcc atcccttcta acacctcagg ccgcgtatag tgcgggcaat
     3481 ccagcaatcc ttcagcaaag gaatcttccg ttgccgaggc ttcatgtccc agtactcccg
     3541 gaataaaccg ggaaacggag tcaatcagcg tcattgctgg taactcacca ccactgagaa
     3601 cgtaatcgcc gattgaccat tcttcgtcaa tttcggtttg gatcacgcgc tcatctatac
     3661 cttcgtagcg accacacacc agaatcaatt tttgattcgt tgccagttcg ctgacgcccg
     3721 cttgatcaag cttgcgtccc tgtggtgaca gataaatcac ctttgcgcct tcacccgccg
     3781 cggcttttgc tgcatgaatg gcgtcccgca agggttgcac catcattaac atccccggtc
     3841 cgccgccgta aggacgatcg tccacggtac ggtgccggtc atgcgtgaag tcacgaggac
     3901 tccagctctg gatgctcagc aggccatttt taactgcccg gccagttacc ccgtaatcgg
     3961 taattgcgcg gaacatttca ggaaacaggc taattatgcc aatccacata gcgccgtctt
     4021 ttaccgttta tccggtggtt taaaaaccag gatcccaatc tacttcgatt gaacgagtag
     4081 tgagatcgac tttcttgata acctgcccat cgaggaacgg tacgagacgt tccttgatac
     4141 caaacgcatc tttcaggttt gccttaatga cgagaacgtc attagatccg gtttccatca
     4201 tatctacgac tttaccgaga tcgtagcctt cagtggttac tacctggcag cccatcaggt
     4261 ctttccagta gtagtccccc tcttcaagct gaggcaactg cgatgaatcc acgacaattt
     4321 cacaattcgt cagcaggttc gccgcatcac gatcgtcaac gcctttcagc ttgatgatca
     4381 tgtcctgatt gtggtgcttc cagctttcca gctggacttg ctgccactga cccgccttct
     4441 ggataaacca gggctgatag tcaaaaatgc tttcggcgtc ttcggtggaa gaaaacactc
     4501 tgagccaccc acgaataccg taagacgaac ccatttttcc caaaacgatg ggatcaacag
     4561 gtgcttgcgc ggtgagttgt ttgctcatca tgaccaccgt gacagattaa gctgctttgt
     4621 ttacttcttt gatcagcgca gcaacgcgat cagaaatagt tgcgccctgg ccaacccagt
     4681 gagcgatgcg atccagatcc aggcgagtgc cttcttcttt ttcgctagcg attgggttga
     4741 agaaaccaac gcgctcaatg aagcgaccgt tgcgtgcatt acggctgtca gcgacaacaa
     4801 cctggtagaa cggacgcttt ttagcgccgt gacgtgctaa acgaatagtt accataacat
     4861 cctcttgtgt gaataaaaca accggacccc atcgaggaac ggagtccggt gtcatattaa
     4921 aagcccgaaa attttactga tttctggaga aaatgcaatc agcagttaat aactctgtcg
     4981 cataaggtca tttagcgacc aggaaatccc ggcggcatca tccctttcat gcttctcatc
     5041 atcttcgcca tcccgccctt cttcattttc ttcatcatgc gctgcatgtc gtcgaactgt
     5101 ttcagaagac ggttaacgtc ctgcacctgc ataccgcaac cggcagcgat acgacgctta
     5161 cgcgaacctt tgatgatttc tggcttagcg cgctctttca tcgtcatcga gttgatgatg
     5221 gcttccatac gcaccagcac tttatcgtcc atctgtgact tgacgttatc cgggatctgc
     5281 cccatgcccg gcagcttgcc catcaggcta gccatgccgc ccatattttt catctggcgc
     5341 agctgctcaa gaaagtcgtt gagatcgaag ccgtcgcctt ttttcagctt gctggctaat
     5401 ttctctgcct gcgcgcggtc aaccttgctt tcgatatctt cgatcagtga cagtacgtcg
     5461 cccatgccga gaatacggga ggcaatacgg tccggatgga atggctccag cgcctcggtt
     5521 ttctcgccaa caccgaggaa cttgatcggt ttgccagtga tgtgacgaat agagagcgcc
     5581 gcaccgccgc gggcatcgcc gtccactttg gtcaacacta caccggtaag cggtaacgct
     5641 tcattgaatg cttttgccgt attggccgca tcctgaccgg tcatggcatc gaccacaaac
     5701 agggtttcaa ccggattaat cgacgcatgg acttgtttga tctcgtccat catcgcttcg
     5761 tcaacgtgca gacgaccggc agtatccacc agcagcacgt cgtagaattt cagtttggct
     5821 tctttcagcg ccgcgttaac gatatctacc ggcttctgac caacatcaga agggaagaaa
     5881 tcaacgccca cctgctctgc cagcgtctca agctgtttga ttgccgccgg gcgatagacg
     5941 tcggcagaaa ccaccagcac tttcttcttg tgcttctcgc gcaggaactt accgagttta
     6001 ccaacgctgg ttgttttacc ggcaccttgc aggcccgcca tcagcacgac cgcaggcggt
     6061 tgcgcagcca ggttcagggt ctggttctct tcgcccatcg ccgcaaccag ttcgttacgg
     6121 acaattttga cgaactcctg ccccggcgtc aggctcttat taacttcatg accaaccgct
     6181 ttctctttta cgcgattgat aaactcacgc actaccggca gagcgacgtc cgcctccagc
     6241 agcgccatgc gcacttcgcg cagcgtatct tttacgttgt cttcagtgag gcgtccacgg
     6301 ccactgatat tgcgcagcgt gcgcgacaaa cgatcggtta aattatcaaa cattgtctct
     6361 cgcctggggt ggaaacggtt ggccgcaatc gcgacacatc atcagtattt tgccgcagta
     6421 taacatgaag gcgtctttgt tgttatgcaa cggttggagc agcgttcacc tgacgctata
     6481 ctgcttctct ttcttattgc tcaaactgtc gacattacta tgcccgtttt tgctctgctc
     6541 gcgcttgtcg cctactccgt cagtcttgcg ctgattgttc ccggtctgct gcaaaaaaac
     6601 ggcggctggc ggcgcatggc tattatttct gcggtcatcg cgctggtctg ccacgcaatc
     6661 gctctggaag cccgcatcct gcccgacggt gatagcggac aaaacctcag cctgctgaac
     6721 gttggttcat tggtcagttt gatgatctgt acggtaatga ccattgtggc ttctcgcaat
     6781 cgtggctggt tgttgctacc cattgtctat gcctttgcgc ttatcaacct ggcgctggcg
     6841 acctttatgc ccaatgaata catcacccat ctggaagcaa cgccggggat gctggtgcac
     6901 attggcttat cgcttttttc ctatgccacg ctaattatcg ctgccctgta cgcgctgcaa
     6961 ctggcgtgga ttgattacca actgaagaac aagaagctgg cgtttaacca ggaaatgcct
     7021 ccattgatga gtatcgagcg taaaatgttc catatcacgc agattggcgt ggtgctgcta
     7081 acgctcactc tttgcactgg cctgttctac atgcacaact tatttagcat ggaaaatatc
     7141 gacaaggccg tgctctctat cgtggcgtgg tttgtctata ttgtgctgct gtggggacat
     7201 tatcatgaag gatggcgtgg acgccgcgtc gtctggttta acgttgcggg tgcagtcatt
     7261 ctgacactgg cctacttcgg cagccgaatt tttcagcagt taatcagcta aacccagaaa
     7321 aggagtttcc cctggaacac atttctacta ctacgttgat cattattctg atcatcatgg
     7381 tggtcatttc agcctatttt tccggttcag aaactggaat gatgacactc aaccgctatc
     7441 gcctgagaca catggcgaag caaggtaatc gctcggcaaa acgcgtcgaa aaattactgc
     7501 gcaagccaga ccgcctgata agcctggtgt taatcggcaa taatctggtc aatatccttg
     7561 cttccgcact cggcactatt gtcgggatgc gcttgtatgg cgatgctggc gtggcaattg
     7621 cgactggcgt gctgaccttc gtagtgctgg tttttgctga agtattgccg aaaaccattg
     7681 ccgcactgta cccggaaaaa gtcgcctatc cgagtagttt tctactggcg ccgctacaag
     7741 tcctgatgat gccgctagtc tggttactaa atgccatcac ccgaattttg atgcgtatga
     7801 tgggtattaa aaccgatatc gtagtcagcg gctctttgag taaagaagag ttgcgcacca
     7861 tcgtgcacga atcacgctca caaatttccc gtcgcaatca ggatatgctg ctgtcggtgc
     7921 tcgatctgga aaagatgacc gtcgatgaca tcatggtgcc gcgcagtgaa attatcggta
     7981 ttgatatcaa cgatgactgg aaatcgattc tgcgccaact ctcccactca cctcacgggc
     8041 gcatcgtgct ctaccgtgat tcgctggacg acgccatcag tatgctgcgt gtgcgtgaag
     8101 cctggcgact catgtcggag aaaaaagagt tcaccaaaga aaccatgcta cgcgccgcgg
     8161 acgagatcta ttttgtcccg gagggtacgc cgctcagcac acagttggtg aagtttcagc
     8221 gcaacaaaaa gaaagtcgga ctggtcgtca acgagtatgg agacattcag ggactggtga
     8281 cggtcgaaga tattctcgaa gagattgtcg gcgatttcac cacgtcgatg tcgccaacac
     8341 ttgccgaaga ggtcacgccg caaaacgacg gttcggtgat tattgatggc actgccaacg
     8401 tgcgagaaat taacaaagcc tttaactggc atctaccgga agatgatgct cgtactgtta
     8461 acggcgtcat tctggaagcg ctggaggaga tcccggtagc aggcacccgc gtgcgtatag
     8521 gcgagtacga tatcgatatt ctcgacgtac aggacaatat gattaagcag gtaaaagttt
     8581 ttcctgtaaa accgctgcgc gagagtgtgg cggagtaacg aaaacggccc ggcattcgca
     8641 tgctgggccg tgatttatta cgaaagcaga aattaagctt ttgctttcgc tacagtcacc
     8701 atcgccgcac gaatcgtacg accgttcagc gtataacctt tctgcataat gcccagtacg
     8761 ttacctggcg caacgtcatc agattccacc attgcgatgg cctgatgcac atttgggtcc
     8821 agtgggacgt tggtttcggc gatcacttca acgccaaact tacgcacaac atccagcatc
     8881 gacttcagcg tcagctcaat accttcaacc atcgcggaca tatccgggtt agctttatca
     8941 gccacttcca gcgcccgatc caggctatca atcaccggca gcaattcgtt gatgaatttc
     9001 tccagcgcga atttgtgggc tttttcgata tccagttcag tacgacgacg caggttttcc
     9061 atttcggctt ttacgcgcaa aatgccgtca cgttcacggg tctgggcttc agccagctga
     9121 gcttcgagat tcgcaatttt ttcatcgcgc ggatccacct gctcagcaga agcttctggc
     9181 tcaactgcct caatctcttc gtgctgatcc atgataattt cttccggggc ttgcccctca
     9241 ggcgttttct gttctttact actcatgaat ttctccgcgt ttttttcgca ttcatctcgc
     9301 taacttcgct tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat
     9361 caatcttcgc aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt
     9421 gggacaccca cggcacccca ctgcactgac aacacatgaa atgctctacc gctggctgtg
     9481 cacaaaaggt tacgaggtca tcgttgagca acaaatcgct cacgaactgc aactgaagaa
     9541 tgtgaaaact ggcacgctcg cggagattgg gcaacaggct gatctcgcgg ttgtcgttgg
     9601 cggcgacggt aatatgctgg gcgcggcgcg cacgctcgcc cgctacgata ttaaagttat
     9661 tggaatcaac cgtggcaacc tgggtttcct gactgacctt gatcccgata acgcccagca
     9721 acaattagcc gatgtgctgg aaggccacta catcagcgag aaacgttttt tgctggaagc
     9781 gcaagtctgt cagcaagatt gccagaaacg catcagcacg gcgattaacg aagtggtact
     9841 tcatcctggc aaagtagcgc atatgattga gttcgaagtg tatatcgacg agatctttgc
     9901 gttttcgcag cgttccgatg gactgattat ttcgacgcca acaggctcca ccgcatattc
     9961 cctctctgcg ggcggtccta ttctgacacc ctctctggat gcgattaccc tggtgcccat
    10021 gttcccgcat acgttgtcag cacgaccact ggtcataaac agcagcagca cgatccgtct
    10081 gcgtttttcg catcgccgta acgacctgga aatcagttgc gacagccaga tagcacttcc
    10141 gattcaggaa ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctgattca
    10201 tccgaaagat tacagttatt tcaatacatt aagcaccaag ctcggctggt caaaaaaatt
    10261 attctaattt tacgccagcc tctttactgt atataaaacc agtttatact gtacacaata
    10321 acagtaatgg tttttcatac aggaaaacaa ctatgttggc acaactgacc atcagcaact
    10381 ttgctatcgt tcgtgagctt gagattgatt ttcatagcgg catgaccgta ataactggcg
    10441 agaccggcgc gggtaaatct attgcaatag atgccctcgg cctttgtctc ggtggtcgcg
    10501 ctgaagccga catggtgcgt accggcgctg ctcgtgccga cctgtgcgcc cgtttttctc
    10561 tgaaagatac gccagcggcc ctgcgttggc tggaagaaaa ccagcttgaa gacgggcatg
    10621 aatgtttgct tcgtcgcgta atcagcagcg atggtcgctc ccgtggtttc atcaacggta
    10681 cagctgttcc tctgtcacaa ctgcgcgaac tgggtcagtt gctgattcag atccatggtc
    10741 agcacgctca tcagttactc accaaacccg agcaccaaaa attcctgctt gatggctatg
    10801 ccaatgaaac ctctcagctc caggaaatga ccgcacgtta tcagttgtgg catcaaagct
    10861 gccgtgacct cgcgcatcat cagcagttaa gtcaggaacg cgccgcccgt gcggagttgc
    10921 tgcaatacca attaaaagaa cttaacgaat ttaatccgca gactggagag tttgagcaaa
    10981 tcgacgaaga gtacaaacgt ctggcgaaca gcggtcaatt gctgaccacc agccagaatg
    11041 cattggcatt aatggctgac ggtgaagacg caaatctgca aagtcaactt tacacggcta
    11101 aacaactggt gagcgaattg attggcatgg acagcaaact gtccggcgta cttgatatgc
    11161 tggaagaggc taccatccag attgctgaag ccagtgatga actacgccac tactgcgatc
    11221 gtctggatct cgatcccaac cgactgtttg aacttgaaca gcgcatctca aaacagattt
    11281 cgctggcacg taaacatcac gtcagccctg aggcattgcc acagtattac cagtcgctac
    11341 tggaagaaca gcagcaactg gacgatcagg ccgactcaca agaaacgctc gcgctggcgg
    11401 taacgaaaca tcatcagcag gcactggaaa cggcgcgcgc gttacaccag caacgccagc
    11461 attatgcaaa tgagttggca cagctgatca ccgatagtat gcatgcactc tctatgccac
    11521 atgggcagtt tacgatcgat gttaaatttg acgagcatca cctgggcgct gacggcgctg
    11581 atcgtattga gtttcgggta accaccaacc caggtcagcc aatgcagcct attgccaaag
    11641 tcgcatccgg tggtgagttg tcccgcatcg cattggcaat ccaggtcatt accgcgcgta
    11701 aaatggaaac cccggcactg atttttgatg aagtggatgt agggattagc ggcccaacag
    11761 cggcagttgt cggcaaactg ctacgtcaac ttggcgaatc aactcaggtg atgtgtgtta
    11821 cccacctgcc acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg
    11881 atggtgcgat gacagaaacg catatgcaat ccctggataa aaaagcgcgg ttacaagagt
    11941 tggcgcgcct gcttggtggc agtgaagtca cacgtaacac actggcgaat gcgaaagaac
    12001 tgcttgcggc gtaaactttt ttcctgcttc acggtcagag taaacagcaa aacgccgtaa
    12061 gaccggaaag caaaaggttt taaagtgatg aaaggtctat tatcatcggc atattacaga
    12121 tgagccacgt actgctcggg cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg
    12181 actgctgcag cagcagtact attgatgttg accgcaggct gttccactct ggagcgagtg
    12241 gtttaccgtc ctgacatcaa ccaggggaac tatctgaccg ctaacgacgt atccaaaata
    12301 cgtgttggca tgacgcaaca acaagttgcg tacgcattgg gtacaccgct gatgtccgat
    12361 ccatttggta caaatacctg gttctatgtc ttccgccagc aacctggtca tgaaggtgta
    12421 acgcagcaaa cgctgacgct gacctttaac agtagcggtg tgttgaccaa cattgataac
    12481 aaacctgcgc tgagtggtaa ctaatcataa agttgctctc aaagacgtta aaaaaggtgc
    12541 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
    12601 gaagctcttt cggatcggca atgagaggac gataaatctc cacccgatcg ccatcatgca
    12661 caatatcgct gagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
    12721 tatcggtacg caattccagc aaaccactgg cgcgaatagc ttcttcaacc gtcgcgccct
    12781 cctgcaacgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
    12841 caattttacc tggcactgta gacctctttt gcacgaacag taaaagcctg caccatatta
    12901 gctgccagct ctttaaacac acgaccaaag gcgagttcaa tcaacttatt ggtaaactca
    12961 aagtcgagat gaaactcaat acggcacgcg tcctggctca acggcgtaaa cttccaaccg
    13021 ccgatcaatt tcttaaacgg tccatcaacc agactcatga gaatactttg attactggtc
    13081 aactggttgc gggttgtaaa cgttttgctg atcccagcct tagacacatc taccgcagca
    13141 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
    13201 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggcaccagt
    13261 gcggtccggc taatctgagg cataacaatt tccatcaaca aaaatccaac aaataatatc
    13321 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
    13381 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
    13441 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
    13501 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
    13561 gcaaggctgg gaggttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctatgt
    13621 ccttctgcgc gacggagagg catttctgtt tggcgctaac atcacgccaa tggctgtggc
    13681 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
    13741 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
    13801 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta agaaacagca
    13861 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcgc gtatcatgaa
    13921 aaacgcccac cgttaaacct atataagcga tactaatcaa atcctcaccg cgcctcccta
    13981 tccggtggcg cgaatgaaca tcttattgac tgtcaaatct gacacagatg tagccatccc
    14041 attgcttaat cgaaaaaaaa tcatactatg caggcgctaa ttccaggatg ataacgcctt
    14101 ttgggctagt catgctgctc ataaatctgg tatacttacc gttacacatt ggggctgatt
    14161 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
    14221 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
    14281 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
    14341 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
    14401 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
    14461 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccaatcatga
    14521 ttggacggtg taaggactac accaacaaaa acaggaagtt ataagtctca gcaggacacc
    14581 gaccagacgg tgaggagaca aaaaaggata cgcaacggag tcgcgactcc ctatgacatt
    14641 aaagcccgct gatgcgggct tttttattgg tcctgacccg ccctgaatag aattcatcca
    14701 attacagtcc agacatatac agtatccctc agctcagtat cagcttctcg acaaatggtc
    14761 actgttattt acccgttgcc agaaagagaa taatttgccc ccatagccag ccttcccgga
    14821 atctgtatat tcattaatat tgttatcgat tccatcaagt aacataggtt ggatgcgatt
    14881 gatcttactc acattagtcg acacggaact cagtaagttg aactctggaa aagcagttta
    14941 aatagcattt aaacaaatgc cagcagttcg aaaaagagta taagcacact taatatattg
    15001 ttttaattat tcatttcctt tcaacccaat tcttatccct cacattattt gttattaatt
    15061 tgttgttttt gatcgcaata agaaaacaat atgtaacttt tatgcgcatt tttcagaaat
    15121 gtagatattt ttagattatg gctacgaaat gagcatcgcc atgtcaccct acatctcata
    15181 agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa ataacgctgt cgattcaggc
    15241 caggactata gcggattcac tctcatcccg tcggcgcaat ccccgcgtct gctggaactc
    15301 accttcaccg aacagacgac caatcggttc ctcgaacaag ttgccgagtg gcccgtgcag
    15361 gcgctggagt acaaatcgtt tctgcgtttt cgggtaggca aaattctcga cgatctgtgt
    15421 gcgaatcagc tgcaaccgct gctgttgaag accctgttaa accgcgctga aggtgcgctg
    15481 ttgatcaatg cgatgggtat cgatgatgtc gcgcaggcgg atgagatggt gaagctggcg
    15541 acggcggtgg cgcatctgat tggtcgctcc aattttgacg ccatgagcgg tcagtattac
    15601 gcgcgtttcg tggtgaaaaa tgttgataac tcagacagct atcttcgtca gccgcaccgc
    15661 gtaatggagc tgcacaacga cggcacttac gtcgaagaga tcactgatta cgtgctgatg
    15721 atgaaaatcg acgagcagaa catgcagggc ggtaactcgc tgctgctgca tctcgatgac
    15781 tgggaacatc tggacctctt tttccgccat ccgctggcgc gccgtccgat gcgctttgcc
    15841 gcgccgccga gcaaaaacgt cagcaaagat gtcttccatc cggtgttcga tgtcgatcag
    15901 cagggccgcc cggtgatgcg ctatatcgac cagtttgtcc agccgaaaga cttcgaagaa
    15961 ggcgtgtggt tgagcgagct ttccgatgcc attgaaacca gtaaaggcat tctttctgta
    16021 cccgttcccg ttggcaaatt cctgttgatt aacaacctgt tctggctgca cggtcgcgac
    16081 cgctttactc cgcacccgga tctgcaccgt gaactgatgc gtcagcgtgg ctatttcgct
    16141 tacgccactc accactacca gacgcatcag taagcgcaaa ggaattgagc ggatgtatga
    16201 ttttgtgatt attggcggcg gcatcatcgg catgtcgacc gccatgcaac tgattgatgt
    16261 ttatccggat gcccgcattg cgttgctgga aaaagagtcc ggcccggcct gtcaccagac
    16321 gggccacaac agcggcgtaa tccatgccgg ggtctattac acgcccggca gcctgaaggc
    16381 gcagttttgc ctggcaggaa accgcgccac caaagccttt tgcgagcaaa acggcattcg
    16441 ctacgacaac tgcggcaaga tgctggtcgc cacgtctgaa ctcgaaatgg aacggatgcg
    16501 cgccttatgg gaacgcacgg cggcgaacgg aatcgatcgc gagtggttaa acgccgaaga
    16561 gctgcgcgag cgcgaaccaa atatcaccgg gctgggcggt atttttgtgc cgtccagcgg
    16621 cattgtcagc taccgcgaag tgacggcggc aatggcaaaa atttttcagg ccagaggcgg
    16681 tgagattatc tataacgccg aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat
    16741 atgtacccgt cagggcggtg aatatgaagc atcgacgctg attagctgtt ccgggctgat
    16801 ggctgaccgg ctggtgaaaa tgctcggcct cgaaccgggc tttattatct gcccgttccg
    16861 cggcgagtat ttccgccttg cgccggagca taaccagatt gttaaccacc tgatttaccc
    16921 cattcccgac ccggcaatgc cgtttttggg cgtgcatctc acccgcatga tcgacggcag
    16981 cgtgaccgtc gggccaaacg cggtgctggc tttcaaacgc gaaggctatc gtaagcgcga
    17041 cttctcattt agcgacacgc tggaaatttt gggctcgtcg gggattcgcc gggtgctgca
    17101 aaaccatcta cgctcaggac tgggcgagat aaaaaactcg ctgtgcaaaa gcggctatct
    17161 gcggctggtg caaaagtatt gtccccggct ttcgttaagc gatctccagc cctggccccc
    17221 cggtgtgcgg gcgcaggcag tatcgccgga cggcaagctg attgacgatt ttctgtttgt
    17281 caccaccccg cgcacgatcc acacctgtaa tgcgccctcc ccggcagcga catcagcaat
    17341 tcctattggt gcgcatattg tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc
    17401 cggacgcacg ctgcgagcgg cacgtagtgt ggatgcctta cacgccgcat tttatcaata
    17461 acctttgaaa acaggatgta gcgatgaaac ttaacgacag taacttattc cgccagcagg
    17521 cgttgattaa cggggaatgg ctggacgcca acaatggcga ggtcatcgac gtcaccaatc
    17581 cggcgaacgg cgacaagctg ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg
    17641 ctatcgacgc cgccaaccgc gccctgcccg cctggcgtgc gctcactgcc aaagaacgcg
    17701 ccaacattct gcgcaactgg ttcaatttaa tgatggagca tcaggacgat ttagcgcgcc
    17761 tgatgaccct cgaacagggc aaaccactgg ctgaagcgaa aggcgaaatc agctacgccg
    17821 cctcatttat tgagtggttt gcagaagaag gcaaacgtat ttatggcgac accattcccg
    17881 gtcatcaggc cgataaacgc ctgattgtta tcaagcagcc gattggcgtc accgcggcta
    17941 tcacgccgtg gaacttcccg gcggcgatga ttacccgtaa agccggtccg gcgctggcgg
    18001 caggctgcac gatggtgctg aagcccgcca gtcagacgcc gttctctgcg ctggcgctgg
    18061 cagaactggc gattcgcgcg ggcgttccga ctggcgtgtt taacgtggtc accggttcgg
    18121 cgggcgcggt cggtaacgaa ctgaccagca acccgctggt gcgcaagctg tcgtttaccg
    18181 gctcgaccga aattggtcgc cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt
    18241 cgctggagct gggcggcaac gcaccgttta ttgtctttga cgatgccgat ctcgataaag
    18301 ccgtggaagg cgcgctggcc tcgaaattcc gtaacgccgg gcaaacctgc gtctgcgcca
    18361 accgcctgta tgtgcagaac ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg
    18421 tgagcaaact gcacatcggc aacgggctgg agaaaggcgt caccatcggg ccgctgatcg
    18481 atgaaaaagc ggtagcaaaa gtggaagagc atattgccga tgcgctggag aaaggcgcgc
    18541 gtgtggtttg cggcggtaaa gcacatgaac gcggcggtaa cttcttccag ccgaccattc
    18601 tggtggacgt tccggctaac gccaaagtgt cgaaagaaga gacgtttggc cccctcgccc
    18661 cgctgttccg ttttaaagat gaagccgatg tgattgcgca agccaatgac accgaatttg
    18721 gacttgcagc ctatttctac gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcac
    18781 tggagtacgg catcatcggc atcaacaccg gcattatttc caatgaagtg gccccgtttg
    18841 gcggcattaa agcctcgggt ctgggtcgtg aaggttcgaa gtatggcatc gaagattact
    18901 tagaaatcaa atatatgtgc atcggtcttt aactggagaa tgcgaatgag cagcaataaa
    18961 gagttaatgc agcgccgcag tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt
    19021 ttcgctgacc gcgcggaaaa ctgccgggtg tgggacgttg aaggccgtga gtatcttgat
    19081 ttcgcgggcg ggattgcggt gctcaatacc gggcacctgc atccgaaagt ggttgcggcg
    19141 gtggaagcgc agttgaaaaa actgtcgcac acctgcttcc aggtgctggc ttacgagccg
    19201 tatcttgaac tgtgcgagat tatgaatcag aaggtgccgg gcgattttgc caagaaaact
    19261 ctgctggtta cgaccggttc cgaagcggtg gaaaatgcgg tgaaaatcgc ccgcgccgcc
    19321 accaaacgta gcgggaccat cgcctttagc ggtgcgtatc acgggcgcac gcattacacg
    19381 ctggcgctga ccggcaaggt gaatccgtac tctgcgggca tggggctgat gccgggtcat
    19441 gtttatcgcg cgctttatcc ttgcccgctg cacggcataa gcgaggatga cgctatcgcc
    19501 agcatccacc ggatcttcaa aaatgacgcc gcgccggaag atatcgccgc catcgtgatt
    19561 gagcccgttc agggcgaagg cggtttctac gccgcgacgc cagcctttat gcagcgttta
    19621 cgcgcgctgt gtgacgagca cgggatcatg ctgattgccg atgaagtgca gagcggcgca
    19681 gggcgtaccg gcacgctgtt tgcgatggag caaatgggcg tggcaccaga tctcaccacc
    19741 tttgcgaaat cgatcgcagg cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg
    19801 atggatgccg tcgctccagg cggtctgggc ggcacctatg ccggtaaccc gattgcctgc
    19861 gtagcggcgc tggaagtgtt gaaggtgttc gagcaggaaa atctgctgca aaaagccaac
    19921 gttctggggc agaagctgaa agacggattg ctggcgatcg ccgaaaaaca cccggagatc
    19981 ggcgacgtac gcgggctggg ggcgatgatc gccatcgagc tgtttgaaga tggcgatcac
    20041 agcaaaccgg acgcaaaact caccgccgag atcgtggcac gcgcccgcga taagggtctg
    20101 attcttctct cctgcggccc gtattacaac gtgctgcgca tccttgtacc gctcaccatt
    20161 gaagacgctc agatccgtca gggtctggag atcatcagcc agtgttttgc tgaggcgaag
    20221 cagtagcacg gcaccatgcc gggggcgacg ctttgcagct tgtccggact acgagaatcc
    20281 aggtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg aataaatcta
    20341 tgtcggccgg ataaggcgga gccgctctcc ggcaaaaaga accaataaca attaaacgcg
    20401 tgacccgacg cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca
    20461 ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaagtca cgccacgtca
    20521 ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca
    20581 tcgccgaagc gggcccggcg gtattactgg cctatctgtt cgccggatta ctggtggtta
    20641 tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt tcgttttcca
    20701 cctatgccga taaagccatt ggccgctggg cgggctatac catcggctgg ttgtactggt
    20761 ggttttgggt actggttatc ccacttgaag ccaacatcgc cgccatgatc ctgcactcgt
    20821 gggttccagg cattcccatc tggttatttt ccctcgtcat taccctcgcc ttaaccggca
    20881 gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgtaaag
    20941 tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt ttttacccgt
    21001 atgccgacgt gagcggtatt tcaagattgt gggatagcgg cggctttatg cccaacggct
    21061 tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg
    21121 tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgtccgc gccaccaact
    21181 cggttatctg gcgtatctct attttctatt tgtgttctat ttttgtcgtt gtggcattaa
    21241 tcccgtggaa tatgccaggg ctgaaagccg ttggttctta tcgctcggtt ctggaattgc
    21301 tcaatattcc tcatgcgaaa ttaatcatgg actgcgtgat attactttcc gtaaccagct
    21361 gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc cgtcgcggtg
    21421 atgctcccgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac
    21481 tctccaccgg cgcggcattc ttaacggtgg tggtgaacta ttacgcgcct gcgaaggtgt
    21541 ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg
    21601 tttcacaatt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcctgcgga
    21661 tgtggcttta tccgtggctc acctggctgg taatcggttt tattaccttt gtgttggtag
    21721 tgatgctatt ccgcccggcg caacagttag aagtgatctc caccggctta ttagcgattg
    21781 ggattatctg taccgtgcca attatggctc gctggaaaaa gctgataatg tggcaaaaaa
    21841 cacccattca taatacgcgc tgataatgat caggagtcac accatgacca ttacctctct
    21901 ggatggctat cgctggctga agaacgatat tattcgcggc aattttcagc cggatgaaaa
    21961 attacggatg agtttgctga catcgcgtta tgcacttggc gttgggccgt tgcgggaagc
    22021 gctttcgcaa ctggtggcag aacgactggt cacggtggtg aatcaaaaag ggtatcgggt
    22081 ggcgtctatg tcagagcagg agctgctcga tattttcgat gcccgtgcca atatggaagc
    22141 gatgctggta agcctggcga ttgcccgcgg tagtgatgag tgggaggcag acgttcttgc
    22201 gaaggcgcat ctgctcagta agcttgaggc ctgtgacgcc agtgagaaaa tgcttgatga
    22261 gtgggatctg cgtcatcagg cgtttcatac tgcaattgtg acaggctgtg gttctcacta
    22321 tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg cgctatcgct tcatctggct
    22381 gcggcgaacg gtactttcgg tggaaatgct ggaagataaa cacgatcagc accagaccct
    22441 gactgcgacg gtactggcgc gagataccgc gcgcgccagt gagttaatgc gccagcattt
    22501 gctgacgccg attcccatta tccagcaggc gatggctggc aattaactac tcttcaggaa
    22561 tacgcaacac ttgccccggg taaattttat ccgggctttt tagcatcggt ttattcgctt
    22621 cgaagatttt attgtacaga ttagcgttac cgtagacctg tttggaaatg gcactcagag
    22681 tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc gttttcacct
    22741 gatcatcgac actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg
    22801 cctcctgact taagccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca
    22861 ctttatcggc atcaggtata ccggttttgt tcagatgctc ctgcaccttc ttcgcctgat
    22921 cgtctttatc gtgctgacct gtaaccgcgt cccagagttt ttctccggca tctttcacaa
    22981 aattgaacag acccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga
    23041 ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt taatcgcggg tttgcaccca
    23101 gaacgcgtga atcaaaccgg ggatatagcc aagcagtgtc agcagaatat taataatgaa
    23161 cgcccaaccg aaccctttac cgagcagcac gccgagcggc ggcagaatga tggtgatgac
    23221 gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt taaaaagaga
    23281 ttttatctct taagcgtagt aattttagcg gagcctgcca gtttcagcgt acgcttttac
    23341 tctcatttac gctgctgatg ctggtatatg ttgcactctt agtttagagt ttacttaatt
    23401 tagaaaatac ttaaataact atgactgaac tcgcacaatt acaggccagt gccgaacagg
    23461 cagcggcctt attgaaatca atgagccacc ctaaacggtt gctgattctg tgtatgctta
    23521 gcggttcccc cggcaccagc gcgggagagc tgacgcgcat taccggactg agtgcctctg
    23581 cgacatcaca gcatctcgct cgtatgcggg acgaagggct tatcgacagc caacgggatg
    23641 cccaacgcat tctatattcc attaaaaatg aggcggtaaa tgccattatc gccaccctga
    23701 aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac aaccatttcg ccgcatgatg
    23761 cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat tcgtgatgct gatgaatatc
    23821 ttcgtgaaca tattcctgaa gcagatctag ctccattatc cgtactggaa cagtcaggtc
    23881 ttccgcctaa attacgtcgc gagcaaatta tattccactg ccaggcaggt aaacgcacca
    23941 gtaataacgc cgataaatta gcagcgattg ccgcccccgc agaaatcttt ttactcgaag
    24001 atgggattga tggctggaaa aaagcaggat tgccagtagc ggtaaataaa tcccaatcct
    24061 tgccgttaat gcgccaggtg cagatcgccg cgggtggttt aatattaatc ggcgttgtac
    24121 tgggttatac cgtaaatagc gttttcttct tattaagcgg ctttgttggt gctgggttac
    24181 tgtttgcagg aattagtggt ttttgtggaa tggcaaggtt gttagataag atgccgtgga
    24241 accaacgagc ttgagaagcg acgccggacg cgccatagca gcgacatccg gcctcagtaa
    24301 ttagatcagg aaatcgtcga gagatttacc ttctgccagc gcctgagcga ttggcttcgg
    24361 tgtacggccc tgaccggtcc aggttttagt ttcaccgtta acatcgatga atttatattt
    24421 cgccggacgc ggctggcgtt ttttaccagc gcgtggtgcc gcagcagagc tattacccaa
    24481 taactcttcc gggttaattc cgtcagcttt catcagctcc agccaggtgc taattttttc
    24541 ctggcgctct gccagttcac gctgctgttg ttcttcttct tcacgtcttt ctttagtgac
    24601 aaccctgaat ttttcgagca tttcttcaag aacgtcaatg gagaattcgc gagccatcgc
    24661 acggagggtg cgaatgttat ttaaactttg taacattacg gacataataa agaaaacctt
    24721 ttaacccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta ttatatttcc
    24781 acaaaattta atctatttac agtcctgcac gagattattt cagctgttat tttctgaaac
    24841 tctcagaata atttcaagat tatatattag ccattttgat gagtaatttc ggttattggt
    24901 gaacagagct gctatctctg ctcgataatt ccgcaagcct gacaaaacca aagtgaataa
    24961 aaatgcaact caaccgaata aaagaaaaca tctatcactg gtgctttctg ctgtcaatgt
    25021 acattatctg gcataaaaaa tgctgaaaat gacccggtca ctacatacac cgaattctct
    25081 acgcttatag cgttacctca cccccaaacc ttcatgagtg aaaataaagg taaacacata
    25141 tccaattgat ttaattaaaa ataaatcata ccggcatgta aaaatccaca atgtacaaaa
    25201 aacgcgccgc tacggaatat tttatctaca aaaacagatt aaataaaaat ttttcactaa
    25261 ttgattagta atagccagcg atatacgcta tgcgaaaatg cagatggcaa tgagatccac
    25321 tgctttcatc tccattaaca tcccattacg cttttattaa ggagcattag catgttctca
    25381 ccgcagtcac gcttgcgtca tgcagttgca gatacgttcg cgatggttgt ttactgttct
    25441 gtcgtgaaca tgtgtattga agttttcctc tccggaatga gcttcgaaca gtctttttat
    25501 tccagattgg tagcgattcc ggtgaacatc ttaattgcat ggccatacgg tatgtaccgc
    25561 gatctgttta tgcgcgcggc acgcaaagtt agcccgtcgg gctggataaa aaatctggcg
    25621 gatatcctgg cttatgtgac gttccagtca ccggtgtatg tggcgatctt gttagtggtg
    25681 ggcgcagact ggcatcagat tatggcggca gtcagttcaa acatcgttgt ttcgatgttg
    25741 atgggggcgg tttatggcta cttcctcgat tattgccgcc gactgtttaa agtcagccgt
    25801 taccagcagg taaaagcctg acccttcttt tcgcgactgg catcgccagt cgcgtcttta
    25861 actggcttcg ccaaataaac cattcaaata acgttcaagc gcaacacgcg aactaaagcc
    25921 atgcgggata ccgtaatgct catgacgttc acccgctaaa tagagcggaa attgctggta
    25981 atgatcgcaa gttttaatat cggaagcggg ttcagccagt gttgaactac gttcttttaa
    26041 tgttggatga atcaccaggg cggtacgtcc cattcgcgct tcgcgattaa catagacata
    26101 attttcccca cggcggtaac catacgcttt ctgggttacc acatcgacag taaaaccgac
    26161 tttttcaagt acgcgcgcca cctcgtctgg tcgtaaatac atatttgatc ctcgttatta
    26221 tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa aagtccataa
    26281 agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac atcgaattct
    26341 aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg gcgtgcagga
    26401 tatccagaat gatgtcaatc aattagctga cagtctcgaa tctgtattga agtcctgggg
    26461 cagcgacgcc aaaggggaag ccgaagccgc tcgtagtaaa gcgcaggctc tactgaaaga
    26521 aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg atgccgttgg
    26581 ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagt gtgggtacag cggcagcggt
    26641 gggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcacaa atgataataa
    26701 atacgcgtct ttgaccccga agcctggctt cggggttttt tagcttaagg ctttacgtaa
    26761 ttgttgtgct acctgtcgcg ccataccttc gttaacgata ttagtaaacg aaagcagtaa
    26821 tccaccttcc ccacttgaac ggatacgcca gtcgctaagt gcctgcaccg caaggcctgc
    26881 agcattggct ttacgcgcat gggcaatatc atcgcctatc attctgatca ccatctggat
    26941 accacctttc tgcggcacaa cctgaaatcc ttgctgcgtc aacgcttgct caatccattg
    27001 ccgacgttga gcataatgct gacgcatttt cttcagatgc cgccagaaat gcccctcgcg
    27061 aagaaagtct gccagtgtgt tctgccatag aacaggtacc gcacatggag ccagtgacgc
    27121 ctggtggcgg aattgtgcaa tttgcttcac cggcaccacc agccacgcac agcgcaatgc
    27181 aggaaatagc gctttgctga atgttccggc ataaattacc cgctgcggtg cgtcgagact
    27241 ttttaacgcc ggtaacggct taccgtgata gcgaaactca ctgtcataat catcttcaat
    27301 aatccatgcc tgactacgat ctgcccattc cagtatctga tgcctgcgcg ctaaagagag
    27361 cgccacaccc agcggacttt ggtgtgctgg tgttatcagg gcaaaacgcg catcaggata
    27421 attttgtatt ccgcttgtga tatccagtcc attgtcatca accggcacag gcaaaatttc
    27481 cacatcatgg cgagtgacaa tcgggcgaat cagtggaaag ccgggatctt ctatccacat
    27541 cccgtttccc ggtttcgcca gcgcgtgcag aattaaagct attgaggccg cataaccgtg
    27601 agtgataaag acctgctctg gctgacaatc aatcccacgt gaaacgcgta agtaatcaac
    27661 tattgcctcg cgcaacgccg cctcaccgca gacatcgcct aacgccaaat caaagcgcgt
    27721 ctgggtacga aggcgacgcc ccatcacccg cgcccacagc tctcgcggaa agagatcgag
    27781 tgcgggtaat cccatttgaa atggcaacgg ttgctgactt tcaccggcaa aagctacaac
    27841 ttgtacagta gattgttgcg gatatatccg ctcactgaca aatgtgccag cctgtccccg
    27901 cctgatcagc catccttgcg ccaccagctc agcatacgca ttttcgacag tggatcgaga
    27961 cacccccagc tcctgcgacc aggtacgact tgaaggcagc cttgctccgg gtttcagttc
    28021 cccttgctca atagccgttt ttaactgacg ggcgatatcc tgatagcgcg gcatatggtc
    28081 tgctttttta ggtaatttat ggctctcttt agcagtccat tatagtgcta cattgcgggc
    28141 gttaataaga ggagaaactg tatgactaca ttacgtcagc cttactacga acttagcccg
    28201 gcagtgtata acgcactagt gcaggccaca acggcactgg aaaatagcac gctggatacc
    28261 acgttgatgg agttagttta tttgcgcatc tcgcaaatca acggctgcgc attttgtctg
    28321 gagatgcaca gcaaagcatt gcgcaaatcc ggcgtgccac agcacaaact ggacgccctg
    28381 gcaggttggc gcgtaagcca tcattttgat gaacgcgagc gggcggcgct ggcatgggcg
    28441 gaatcggtaa ccgaaattgc cagaacccat gcggaagacg aggtttatca gcctttgctt
    28501 gagcatttca gcgcagcaga gatcagcgac ttaacgtttg ccatcgggct gatgaattgt
    28561 tttaaccgtc tggccgtttc catgcggatg taacttcttg atgccgggta tttgcccggc
    28621 aaaaaacaca aaatccccta ccccgttgag ccattcatcg gtggcgtaat caatctaaaa
    28681 ttcccatatc ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
    28741 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacgga aatacgaatc
    28801 atgcgcatta ctatttacac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
    28861 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcggca
    28921 gaagcgctgc gtgctcaggg ctttcgtcag ttgccggttg tgattgcagg cgatcttagc
    28981 tggtctggtt tccgcccgga catgatcaat cgtctgcatc cagcgccacg cgcggccagt
    29041 gcatgagcca gctcgtctac ttttccagca gctccgaaaa tacgcagcgt tttatcgaac
    29101 gtttagggct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
    29161 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
    29221 gacaggtcat tcgcttttta aacgacgagc ataaccgggc gttgctgcgc ggcgttattg
    29281 cttcgggtaa tcgcaacttt ggtgaggcat atggccgcgc cggagatgtg attgcccgga
    29341 aatgcagcgt gccgtggctg taccgctttg aactcatggg tacgcaaagc gatatcgaaa
    29401 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaacgcc tgacgcagga
    29461 aacgatggat tatcacgctc tgaatgcgat gcttaacctt tacgatagcg caggtcgcat
    29521 tcagttcgat aaagatcgcc aggccgttga cgcctttatg acgacgcatg tgcgtccgaa
    29581 cagtgtggcc ttcagtagcc agcagcagcg cctgaactgg ctggttaacg aaggttacta
    29641 tgatgaaagc gtccttaatc gctactctcg cgattttgtc attacgctgt ttgcccacgc
    29701 gcacgccagc ggttttcgtt tccagacatt cctcggagca tggaagtttt acaccagcta
    29761 tacgttgaag acattcgacg gtaagcgtta tctggaagat tttgccgatc gcgtcacgat
    29821 ggtggcgctg acgctggcac aaggcgatga aacactggcg acgcagctca ctgatgagat
    29881 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
    29941 cggcgaactg gtttcctgtt ttctgctgcg tattgaagac aatatggagt cgattggccg
    30001 ggcagtaaat tccgcactac aactgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
    30061 gaatctgcgg gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtgat
    30121 tccggtgatg aaaatgctgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
    30181 gggggctggg gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgatac
    30241 aaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
    30301 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
    30361 tgacgtagag cgagtttatg gcaagccgtt tgccgatgtc gccatcagtg aacactatga
    30421 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
    30481 gcgactggca gaaatccagt ttgagtctgg ctatccgtac atcatgtatg aagacacggt
    30541 taaccgtgct aaccctatcg ccgggcgcat aaatatgagc aatctctgct cagaaatttt
    30601 gcaggttaac agcgcctcag aatatgacga gaatctcgac tatgcccgta caggccatga
    30661 tatttcctgc aatctagggt cgttgaatat tgcccacacc atggattccc ccgactttgc
    30721 ccgcacggta gagactgccg tacgcggttt aacggcggtg tcagatatga gtcatatccg
    30781 cagcgtgccg tccatcgaag ccggaaatgc cgcctcacat gccatcggtc tgggacagat
    30841 gaatttacac ggttatctgg cgcgagaagg catcgcttat ggttcgccgg aagcactgga
    30901 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
    30961 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
    31021 atatttcagc caatatctgc aaagtaactg gcagccgaaa acggcgaaag ttggcgaact
    31081 gtttgcccgt agcggtatta cgttacctac ccgtgagatg tgggcgcagc tgcgcgacga
    31141 cgtgatgcgc tatggtatct acaaccagaa tctccaggcg gtgccgccaa ccggttctat
    31201 ctcttatatc aaccatgcca cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
    31261 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
    31321 ggcgctatat caggatgctt acgaaatcgg cgcagaaaag atcatcgaca cctacgcgga
    31381 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
    31441 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aagggtatca aaacgctcta
    31501 ttacattcgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
    31561 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
    31621 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
    31681 aaaggtgccg ctgtcgaacg atattcctgc atggcagaca ttaagtgtcg tagaacaaca
    31741 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
    31801 cgcgccttcg ctgttgcccg atgcactcac gcctcatgaa gaagcggtat tatcgaatat
    31861 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
    31921 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
    31981 agctcagatt attcagcaac attatcgcgg ggatgatccg ctgaaaaaga aaatcgccag
    32041 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt attttgccag
    32101 ccgcggaaag ctaaccaata ccgcagacct gatccgtctg attatccgcg atgaagcagt
    32161 ccacggttac tacatcggct ataaatatca gaaaaacctg gaaaaaatct ccctggcaca
    32221 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactttatg acaacgagtt
    32281 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
    32341 ctgttacaac gccaataagg cgttgatgaa tctgggctac gaaccgttat ttcccgcaga
    32401 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
    32461 cgatttcttt tccggttcag gctcttcata tgtgatggga aaagcggtcg aaacagaaga
    32521 tgaagactgg aatttctgag ggtgttattt ttcaaaaata tcactacccg cagcagggaa
    32581 ataattcccg ccaaatagct ttttatcacg aaaatatttt gtggtgatct acactgacac
    32641 tctgttgcat tattcgcctg aaaccacaat attcaggcgt tttttcgcta tctttgacaa
    32701 aaaatatcaa cttttctcga tttgctctca gcccttatat catgggaaat tccggcgatt
    32761 tgctcacatc aatattcatg ccacatttgc catcaggggt tgcctcagat tctcagtatg
    32821 ttagggtaga aaaaagtgac tatttccatt gggtaatgta tcgacataaa caaataacag
    32881 gaatctttct attgcatggc aattaaatta gaaattaaaa
//
LOCUS       DADTFG010000040        32456 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.040, whole genome shotgun sequence.
ACCESSION   DADTFG010000040 DADTFG010000000
VERSION     DADTFG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.040"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(22..444)
                     /locus_tag="J1138_001742"
     CDS             complement(22..444)
                     /locus_tag="J1138_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBA8453550.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFGGTVQ
                     YMASLGAPMPMLAAVIAVVMEVPAAILIILGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            813..1952
                     /locus_tag="J1138_001743"
     CDS             813..1952
                     /locus_tag="J1138_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBA8453551.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(1944..3224)
                     /gene="csiE"
                     /locus_tag="J1138_001744"
     CDS             complement(1944..3224)
                     /gene="csiE"
                     /locus_tag="J1138_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBA8453552.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQIAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVILGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGATLIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(3415..4269)
                     /locus_tag="J1138_001745"
     CDS             complement(3415..4269)
                     /locus_tag="J1138_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA8453553.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(4414..5217)
                     /gene="suhB"
                     /locus_tag="J1138_001746"
     CDS             complement(4414..5217)
                     /gene="suhB"
                     /locus_tag="J1138_001746"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBA8453554.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            5336..6076
                     /gene="trmJ"
                     /locus_tag="J1138_001747"
     CDS             5336..6076
                     /gene="trmJ"
                     /locus_tag="J1138_001747"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBA8453555.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            6347..6835
                     /gene="iscR"
                     /locus_tag="J1138_001748"
     CDS             6347..6835
                     /gene="iscR"
                     /locus_tag="J1138_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBA8453556.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            6947..8161
                     /gene="iscS"
                     /locus_tag="J1138_001749"
     CDS             6947..8161
                     /gene="iscS"
                     /locus_tag="J1138_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBA8453557.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            8189..8575
                     /gene="iscU"
                     /locus_tag="J1138_001750"
     CDS             8189..8575
                     /gene="iscU"
                     /locus_tag="J1138_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBA8453558.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            8592..8915
                     /gene="iscA"
                     /locus_tag="J1138_001751"
     CDS             8592..8915
                     /gene="iscA"
                     /locus_tag="J1138_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBA8453559.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            9011..9526
                     /gene="hscB"
                     /locus_tag="J1138_001752"
     CDS             9011..9526
                     /gene="hscB"
                     /locus_tag="J1138_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBA8453560.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            9543..11393
                     /gene="hscA"
                     /locus_tag="J1138_001753"
     CDS             9543..11393
                     /gene="hscA"
                     /locus_tag="J1138_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBA8453561.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDAVSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            11395..11730
                     /gene="fdx"
                     /locus_tag="J1138_001754"
     CDS             11395..11730
                     /gene="fdx"
                     /locus_tag="J1138_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBA8453562.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            11742..11942
                     /gene="iscX"
                     /locus_tag="J1138_001755"
     CDS             11742..11942
                     /gene="iscX"
                     /locus_tag="J1138_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBA8453563.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            12001..13284
                     /gene="pepB"
                     /locus_tag="J1138_001756"
     CDS             12001..13284
                     /gene="pepB"
                     /locus_tag="J1138_001756"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBA8453564.1"
                     /translation="MTEAMKITLSPQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGIRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRNQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            13427..14203
                     /gene="sseB"
                     /locus_tag="J1138_001757"
     CDS             13427..14203
                     /gene="sseB"
                     /locus_tag="J1138_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBA8453565.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEGEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRVFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(14700..15545)
                     /gene="sseA"
                     /locus_tag="J1138_001758"
     CDS             complement(14700..15545)
                     /gene="sseA"
                     /locus_tag="J1138_001758"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBA8453566.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYMN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            15752..20713
                     /gene="yfhM"
                     /locus_tag="J1138_001759"
     CDS             15752..20713
                     /gene="yfhM"
                     /locus_tag="J1138_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBA8453567.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNILQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLNLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            20714..23026
                     /gene="pbpC"
                     /locus_tag="J1138_001760"
     CDS             20714..23026
                     /gene="pbpC"
                     /locus_tag="J1138_001760"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBA8453568.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSHERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            23175..23606
                     /gene="ndk"
                     /locus_tag="J1138_001761"
     CDS             23175..23606
                     /gene="ndk"
                     /locus_tag="J1138_001761"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBA8453569.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            23756..24910
                     /gene="trmG/rlmN"
                     /locus_tag="J1138_001762"
     CDS             23756..24910
                     /gene="trmG/rlmN"
                     /locus_tag="J1138_001762"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBA8453570.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            25195..26202
                     /gene="rodZ"
                     /locus_tag="J1138_001763"
     CDS             25195..26202
                     /gene="rodZ"
                     /locus_tag="J1138_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBA8453571.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            26229..27347
                     /gene="ispG"
                     /locus_tag="J1138_001764"
                     /gene_synonym="gcpE"
     CDS             26229..27347
                     /gene="ispG"
                     /locus_tag="J1138_001764"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBA8453572.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            27458..28732
                     /gene="hisS"
                     /locus_tag="J1138_001765"
     CDS             27458..28732
                     /gene="hisS"
                     /locus_tag="J1138_001765"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBA8453573.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            28750..29370
                     /locus_tag="J1138_001766"
     CDS             28750..29370
                     /locus_tag="J1138_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HBA8453574.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            29381..30559
                     /gene="bamB"
                     /locus_tag="J1138_001767"
     CDS             29381..30559
                     /gene="bamB"
                     /locus_tag="J1138_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBA8453575.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            30677..32149
                     /gene="der"
                     /locus_tag="J1138_001768"
     CDS             30677..32149
                     /gene="der"
                     /locus_tag="J1138_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBA8453576.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            32212..32427
                     /locus_tag="J1138_001769"
     CDS             32212..32427
                     /locus_tag="J1138_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453577.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFVIA"
BASE COUNT         7756 a         8016 c         9086 g         7598 t
ORIGIN      
        1 acgccgcatc cggcatcttt cctaacggcg atcgagagaa atcgcccccg gcccggtaat
       61 tgccaacagc aggaatgcgc cagcgatact gacattcttc cagaaattaa tcatatttgg
      121 cccaaccgca tcgccagtca tatcccagta atgatgacca atcaccgcag ttcccagcgt
      181 gtagaaaata aacagcaccg ccagcggacg ggtgaaaaag ccaagtatga ttaatatcgc
      241 ggcgggtact tccataacta ccgcaataac tgccgccagc atcggcattg gtgcgcccag
      301 cgaggccata tattggaccg taccgccaaa gcccatcatt ttgggaaaac caaaaataat
      361 gaaaattaag accacggcga tacgggcaat taataataaa acggggcggg cagtgccaaa
      421 atcaaaataa cgtagtgagt tcataaccaa tccctttgca agatgatgtg atttaaacgt
      481 aatacacact ctggtacctc gccacgccca ggcgttgaga tattcgtttc tggtataacg
      541 gcataacgca gatggctgac aatgtgctta ttgtttcctg ccggatgcga catgcacgcc
      601 ttatccgtct cacatgagtt tgtcagttca ataaattgcg attttcacct gtttcatcgt
      661 tcttttttac atgttgtgtt gtgggccgct cttttttgtc atttcctgat atgcaggata
      721 accgtcagga agcaggctcg aatattctta tttttcaact tgcaccaaca ttcgattaac
      781 ctcagactaa cgcctcctga cgggagggac tcatggtttt gcaatccacg cgctggttgg
      841 cgctcggcta tttcacatac ttttttagtt acggcatttt tctacctttc tggagcgtct
      901 ggcttaaagg gattggttta acgccagaaa ccatcggcct gttattgggg gcgggtctgg
      961 ttgcccgttt cctcgggagt ttgctcatcg cgccccgcgt cagcgatcct tcccgcctga
     1021 tttccgcctt gcgcgtgctg gcactgctga cacttctctt tgctgtcgcc ttctgggcgg
     1081 gggcgcacgt agcgtggctg atgctggtga tgattggctt taacctcttt ttctcaccgc
     1141 tggtaccgtt gaccgatgca ctggcgaata cgtggcaaaa gcagttcccg cttgattacg
     1201 gcaaagtgag actgtggggc tcggtggcgt ttgtcattgg ctcggcgctg acgggcaaac
     1261 tggtcagtat gtttgattat cgggtgatcc tcgcgctgtt gacgttgggc gtggcatcca
     1321 tgctgctcgg ctttctcatc cgtccgacga ttcagccaca aggggcaagc cgccagcagg
     1381 agagcaccgg ctggtctgcg tggttggcgt tggttcgcca gaactggcgc tttctggcct
     1441 gcgtttgttt attgcagggg gcacatgcgg cctattacgg ttttagcgcc atttactggc
     1501 aggcagctgg ctactcggcc tcggcggtgg ggtatttgtg gtcgctgggc gtcgtggcgg
     1561 aagtcattat ctttgcgctg agtaataaac ttttccgccg ttgtagtgca cgcgatatgc
     1621 tgttgatctc ggcgatttgc ggcgtagtgc gctggggcat tatgggagca actacggcgt
     1681 tgccgtggtt gatagtggtg caaattctgc attgcggtac cttcacggtc tgccacctgg
     1741 ctgccatgcg ctatattgct gctcgccagg gtagcgaagt catccgttta caggcggttt
     1801 actctgccgt cgcgatgggc ggcagtatcg ccatcatgac cgttttcgcc ggtttcctgt
     1861 atcaatatct cggccacggc gtgttctggg tgatggcgct ggtggcgctt ccggcaatgt
     1921 ttttgcgccc gaaagttgtt ccctcatgct gattcgagca ttttgcggat ctgctcctgt
     1981 tgatgtgttg tcaacgtcag gtccgtatag atcagcggtg gtgaaaagag cggtaacggc
     2041 atggtataag gcgcaataat cagtgtcgcg ccgcgcggag cgcctgtctg caaaaatgcc
     2101 tttaccgaca tatgtttaat gttgagcggc agtaacgtta gttcgcgtag ctgctgttca
     2161 atctgcgctt ctcgctcgct atcattcccg gtcagtagaa taatctgttt ctcatgcagg
     2221 tcgttttcct gcattagcca ggcacccaaa atcaccgcga ccagaccgct ttcctcatca
     2281 gaaagatgga cgccgtattc actttcaaat ccggccagcg ccgcgcgggt ggtgcgcacc
     2341 aggcgtggat acaggcgggc gaactcttcc ggcagggtgt tatcaatacc gatggcaaac
     2401 aaactgcgat ttaacgcctg ggcgaggtgg gtataaagct gatcgcataa cccctgttca
     2461 tcataaaaac gaacatttcc cagctcacga aaatggttta ccagacgttt gatagactga
     2521 cgcagttgtc tgtcccgctg atgcgcatcc cgcagtggat cgggaacccg caacatggaa
     2581 aaaagtagtg ccataaatag tggctcatca ggcggtacag gctggagcgc ccgacgctgc
     2641 cagtggcgtc caatttcctg cgctatctga aattcaaggc aggattctgc ccagcgacgt
     2701 tggagcggat taaactgagg cgtaattcca gcgtgatgtt gcaacaaaca atattgcaaa
     2761 tacagacaca ggaaatgaat atcgcgcgtt tcaaagcgtt tttgcagccg tcgagagcag
     2821 agattcacta acgcttgtag attggtatcg tcgtaaaagt tacgcgcaat tccgcgccgc
     2881 ttcagttcac tcttcagggc gggggtgaat cggctggtaa taaatgacgg acacagacgg
     2941 aaaccacgtc gtagccagtg aaataaacac aaacgttggt taagcactgt accttcaacc
     3001 cggtagctgc cgtcataacc agtagtcagt gtgagttgat gatagcgcag gatctcccgt
     3061 cctgtttcgc tgatatcaag actggcaata tcatcatcca caccattaag ctcgctgaaa
     3121 gttgccgtgg tggcggtcaa ccccggctga aagagtgtca gcaagatctg gcagcggcgc
     3181 tggggagccg aaaggacaga tggtggagca agcgtaggca tcatctgtag atctcccata
     3241 agcataattg ataagaatag ctaaagtttg ctcagggaaa tggcgaaggg aatgctaatc
     3301 atcagaaatg ttggcaagca tcacagaatt tttctcatct taaactgttc tgaccagtgg
     3361 attagcatgt cttaaggtgg ttttgtgaat gattatcgtg ctagcgatca taggttagtt
     3421 ctgcggattc aatgcgctaa ggataaaatc caccatctgt tcgcgataaa catcgccgtg
     3481 acgatcggaa aaagcatcaa tgtgttcgcc atccgggatt aggatcaacc gttttggttc
     3541 ttttgccagg ctatacaact tttcactgtg ctgccacggg ataacgtgat cagctttacc
     3601 gtgaatgagt aaaagcggga tcgggctgac gctggcgatg taattttcgc cgctgtaact
     3661 ctcatcaagt aagtagccac tgccggggat catctggttg gcgatggtcg cataagaggc
     3721 aaaggtggag tcgaggatca ccgcacgtat gccttcacga tcatcctgac caataacatc
     3781 cagaatattc gccccgccaa tactctgccc gaacagcacc agccgttgtg ggtttacatc
     3841 gctgcgatgg cgcaccacat tgatggcact ttgcgtatcg tccagcaatc cggcctggga
     3901 cggcgtgcct tttgatttac caaacccgcg ataatcaaac ataaaaacgt tgaagttacg
     3961 ctcgggtaac caactgacca gcggccagtg ggcggacata tttccggcat tgccgtgagc
     4021 atgaatgatg gttgcgatgg cgttgtcagc agggcccgtc gaagaaggaa taaaccagcc
     4081 ttgcagacga gtaccgtcct tagccgtaaa ttcgacggat tccgccgacc aggggtcggg
     4141 accgtatatt ttatcgtcag gataatagaa gaagttgatg gcgacgcgcg gcactaaata
     4201 aacgcagaag gcaacaacaa agagaataaa cagaattttg agaacgcgtt tgttcactgg
     4261 cagcgccata acctttcctt gcgacttctt tatcatccat taatcgggga aagatggtgg
     4321 gagagtgacg aaagaaagta aagcggtgag gaagtatttc ggaatagcgc ctgaaaggcg
     4381 agggcgggtg agtgatatca cccgcctgag tcattaacgc ttcagagcgt cgcttaactc
     4441 gtcacgcatg ttcgccagca tggctttaac aacgcgcggg ttaccagcaa cgatgttacc
     4501 ggtcagcata tagttatgac caccggtgaa gtcgctgacg atgccgcctg cttcacgaac
     4561 cagcagctcg cctgccgcga aatcccacgg gcgcagaccg atttcaaaga aaccgtcaac
     4621 acgacccgca gcgacgtaag ccagatccag cgccgcagaa ccggtacgac ggaagtctgc
     4681 acattcgttg aacagtttgc cgacgatgtt gatgtaggta gtggcgtact gttttgcttt
     4741 gaacgggaag ccggtcgcca gaatagtacc gtcgagatcg cgagcggtgc tgccgcgcag
     4801 acggtagccg ttcagctgtg cgccctgacc gcgagtggcg gtgaacagtt cgttacgcat
     4861 aggatcgtat accacagcaa cttcggtgcg gcctttgata cgaacggcga tagataccgc
     4921 gaagtgcggc agacgtttga taaagttggt agtgccatcc agtggatcga taacccattg
     4981 aacatcctga tcagtacctt caagttcacc gctttcttcg gtgatgatgg tgtgctgtgg
     5041 gtaagattta cgaatcgtgt cgataatcac cgcttcggca gctttatcta cgttggtcac
     5101 gaaatcgtta ctgcctttct ggctcgcttc tacagcgtcc ggggtttcat agtttttggc
     5161 aattaaatta cccgccttgc gcgctgcgcg cacggcgatg ttcagcatcg gatgcatcgg
     5221 tctctctcac tggatgttaa agaacgggaa aacggcgcag agtatagcag cgagatcggc
     5281 atctgtcttc gttttatgat aagatgcgtg aataatcttt agatgaagaa agacaatgct
     5341 gcaaaatatt cgaattgtgc tggtggagac gtcacacacc ggcaatatgg gttctgttgc
     5401 ccgtgccatg aaaacaatgg gattaaccaa tctgtggctg gttaatccac tagtgaaacc
     5461 agactcccag gcgattgccc tggcagcagg ggccagcgat gtgattggta atgctcacat
     5521 cgtcgatacg ctcgacgaag cgttagccgg ttgtagtctg gtggtgggca ccagcgctcg
     5581 ctcccgcacg ctgccgtggc cgatgctcga cccgcgcgaa tgcggtctga aaagcgtcgc
     5641 tgaagcggca aataccccgg tggcgctggt gtttggtcgc gagcgcgtcg gcttgaccaa
     5701 tgaagagttg cagaaatgcc attatcatgt cgcgattgcg gctaacccgg aatacagttc
     5761 gctgaacctg gcgatggcgg ttcaggttat tgcctatgaa gtgcgcatgg cctggctggc
     5821 aactcaggaa aatggcgagc aggtcgagca tgaagagacg ccgtatccgc tggtcgatga
     5881 tctggagcgt ttttatggtc atctggagca aacgctgctg gcaaccggtt ttatccgtga
     5941 aaaccatccg gggcaggtga tgaataaatt gcgccgtctg tttacccgtg cacgcccgga
     6001 aagccaggaa ttgaatatcc tgcgcgggat tctggcttct attgagcagc agaataaagg
     6061 taacaaggcc gaataacagt ggttgcctga tgcgatgctt aacgcgtctt atcaggcctg
     6121 taagtgcacg gaatccaagg tcggataagg tgttcacacc gcatccgaca acaggtacaa
     6181 acgccacgat aaaaaaatgg cactgaaggt taaatacccg actaaatcag tcaagtaaat
     6241 agttgaccaa tttactcggg aatgtcagac ttgaccctgc tatgcaatac ccccactttt
     6301 acaataaaaa accccgggca ggggcgagtt tgaggtgaag taagacatga gactgacatc
     6361 taaagggcgc tatgccgtga ccgcaatgct tgacgttgcg ctcaactctg aagcgggccc
     6421 ggtaccgttg gctgatattt ccgaacgtca gggaatttcc ctttcttatc tggaacaact
     6481 gttttcccgt ctgcgtaaaa atggtctggt ttccagcgta cgtggaccag gcggtggtta
     6541 tctgttaggc aaagatgcca gcagcatcgc cgttggcgaa gtgattagcg ccgttgacga
     6601 atctgtagat gccacccgtt gtcagggtaa aggcggctgc cagggcggcg ataaatgcct
     6661 gacccacgcg ctgtggcgtg atttgagcga ccgtcttacc ggttttctca ataacattac
     6721 tttaggcgaa ctggttaata accaggaagt gctggatgtg tctggtcgtc agcatactca
     6781 cgacgcgcca cgcacccgca cacaagacgc gatcgacgtt aagttacgcg cttaataaaa
     6841 agaattcaga atcaggccgg agtgctaaac actccgtaaa cacggtcgta catccagccg
     6901 gtagcctggt tccttgcatt gagtgatgta cggagtttat agagcaatga aattaccgat
     6961 ttatctcgac tactccgcaa ccacgccggt ggacccgcgt gttgccgaga aaatgatgca
     7021 gtttatgacg atggacggaa cctttggtaa cccggcctcc cgttctcacc gtttcggctg
     7081 gcaggctgaa gaagcggtag atatcgcccg taatcagatt gccgatctgg tcggcgctga
     7141 tccgcgtgaa atcgtcttta cctctggtgc aaccgaatct gacaacctgg cgatcaaagg
     7201 tgcagccaac ttttatcaga aaaaaggcaa gcacatcatc accagtaaaa ccgaacataa
     7261 agcggtactg gatacctgcc gtcagctgga gcgcgaaggt tttgaagtca cttacctggc
     7321 accgcagcgt aatggcatta tcgacctgaa agaactcgaa gcagcgatgc gtgacgacac
     7381 catccttgtt tccatcatgc acgtaaataa cgaaatcggc gtggtgcagg atatcgcggc
     7441 tatcggcgaa atgtgccgtg ctcgtggcat tatctatcac gttgatgcaa cccagagcgt
     7501 gggcaaactg cctatcgacc tgagccagtt gaaagttgac ctgatgtctt tctccggcca
     7561 caaaatctat ggcccgaaag gtattggtgc gctgtatgtg cgtcgtaaac cgcgcgtacg
     7621 tatcgaagca caaatgcatg gcggcggtca cgagcgcggt atgcgttccg gcactctgcc
     7681 tgttcaccag atcgtcggta tgggcgaagc ctatcgcatc gcaaaagaag agatggcgac
     7741 cgagatggaa cgtctgcgcg gcctgcgtaa ccgtctgtgg aacggcatca aagatatcga
     7801 agaagtttac ctgaacggtg acctggaaca cggtgcgccg aacattctca acgtcagctt
     7861 caactacgtt gaaggtgagt cgctgattat ggcgctgaaa gacctcgcgg tttcttcagg
     7921 ttccgcctgt acgtcagcaa gcctcgaacc gtcctacgtg ctgcgcgcgc tggggctgaa
     7981 cgacgagctg gcacatagct ctatccgttt ctctttaggt cgttttacta ctgaagaaga
     8041 gatcgactac accatcgagt tagttcgtaa atccatcggt cgtctgcgtg acctttctcc
     8101 gctgtgggaa atgtacaagc agggcgtgga tctgaacagc atcgaatggg ctcatcatta
     8161 atcggtatcg gaatcaggag aatttataat ggcttacagc gaaaaagtta tcgaccatta
     8221 cgagaatccg cgtaacgtgg gttcctttga caacaacgac gagaacgtcg gcagcggcat
     8281 ggtgggtgca ccggcctgtg gcgacgtgat gaagttgcag attaaagtca acgatgaagg
     8341 tatcattgaa gacgcgcgtt ttaaaactta cggctgcggt tccgctatcg cttccagctc
     8401 cctggtcacc gaatgggtga aagggaagtc tctcgacgaa gcacaggcga taaaaaacac
     8461 cgatattgct gaagaacttg aactgccgcc ggtgaaaatt cactgttcta ttctggcaga
     8521 agacgcgatc aaagccgcca ttgcggacta taaaagcaaa cgtgaagcaa aataagagtt
     8581 gaggtttggt tatgtcgatt acactgagcg acagtgcagc agcgcgagta aatacctttc
     8641 tggctaaccg cggtaaaggg tttggcctgc gtctgggcgt gagaacctcc gggtgttcag
     8701 gtatggctta tgtactggaa tttgttgacg aaccgacgcc ggaagacatc gtgtttgaag
     8761 acaaaggcgt gaaagtcgtg gtcgatggca aaagcctgca atttctggac ggtacgcagc
     8821 tggacttcgt caaagaaggc cttaacgaag ggtttaaatt caccaacccg aacgtcaaag
     8881 atgagtgtgg ttgcggcgaa agcttccacg tttgatgcgc ataccgataa ccccaccgtg
     8941 gtcgcctgcg cgtggggttt gttttacctg attcgccgtt atcgcggcgg atcgcagccc
     9001 tgagaatgtt atggattact tcaccctctt tggcttgcct gcccgttatc aactcgatac
     9061 ccaggcgctg agcctgcgtt ttcaggatct acaacgtcag tatcatcctg ataaattcgc
     9121 cagcggaagc caggcggaac aactcgccgc cgtacagcaa tccgcgacca ttaaccaggc
     9181 ctggcaaacg ctgcgtcatc cgttaatgcg cgcggaatat ttgctttctt tgcacggttt
     9241 tgatctcgcc agcgagcagc atactgtgcg cgacaccgcg ttcctgatgg aacagctgga
     9301 actgcgcgaa gaactggacg agatcgaaca ggcgaaagat gaagcgcggc tggaaagctt
     9361 tatcaaacgt gtgaaaaaga tgtttgatac ccgccatcag ttgatggttg aacagttaga
     9421 caacgagacg tgggacgcgg cggcggatac cgtgcgtaag ctgcgttttc tcgataaact
     9481 gcggagcagt gccgaacaac tcgaagaaaa actgctcgat ttttaatttc tggaagctaa
     9541 acatggcctt attacaaatt agtgaacctg gtttgagtgc tgcgccgcat cagcgtcgcc
     9601 tggcggccgg tattgacctg ggcacaacca actcgctggt ggcaacagtg cgcagcggtc
     9661 aggccgaaac gttagctgac catgaaggcc gtcacctgct gccatctgtt gttcactatc
     9721 aacagcaagg gcattcggtg ggttatgacg cgcgtactaa cgcagcgctc gacaccgcca
     9781 acaccattag ttctgttaaa cgcctgatgg ggcgctcgct ggctgatatc cagcaacgct
     9841 atccgcatct gccttatcaa ttccaggcca gcgaaaatgg cctgccgatg attgaaacgg
     9901 cggcggggct gctgaacccg gtgcgcgttt ctgcggacat cctcaaagca ctggcggcgc
     9961 gggcaactga agccctggca ggcgagctgg atggtgtcgt tatcaccgtt ccggcgtact
    10021 ttgacgatgc ccagcgtcag ggcaccaaag acgcggcgcg tctggcgggg ctgcatgttc
    10081 tgcgcttact taatgaaccg accgctgcgg ctattgccta cgggctggat tccggccagg
    10141 aaggcgtgat cgctgtttat gacctcggcg gcgggacgtt tgatatttcc attctgcgct
    10201 taagtcgcgg cgtgtttgaa gtgctggcga ccggcggtga ttccgcgctc ggcggcgatg
    10261 atttcgacca tctgctggcg gattacattc gcgagcaggc gggcattcct gatcgtagcg
    10321 ataaccgcgt tcagcgtgaa ctgctcgatg ctgccattgc ggccaaaatc gcgctgagcg
    10381 atgcggtctc cgtgaccgtt aacgttgcgg gctggcaggg cgaaatcagc cgtgaacaat
    10441 tcaatgaact gatcgcgcca ctggtaaaac gaaccttact ggcttgtcgt cgcgcgctga
    10501 aagatgcggg cgtagaagct gatgaagtgc tggaagtggt gatggtgggc ggttctactc
    10561 gcgtgccgct ggtgcgtgaa cgagtaggcg aatttttcgg tcgtccaccg ctgacttcta
    10621 tcgacccgga taaagtcgtc gctattggcg cggcgattca ggcggatatt ctggtgggta
    10681 acaagccgga cagcgaaatg ctgttgctgg atgtgatccc gctgtcgctg ggcctcgaaa
    10741 cgatgggcgg cctggtggag aaagtgatcc cgcgtaatac cacgattccg gtggcccgcg
    10801 cccaggattt caccaccttt aaagatggtc agacggcgat gtctatccat gtgatgcagg
    10861 gtgagcgcga actggtgcag gactgccgct cgctggcgcg ttttgcgctg cgaggtattc
    10921 cggcgctacc ggctggcggt gcgcatattc gcgtgacgtt ccaggtcgat gccgacggtc
    10981 ttttgagcgt gacggcgatg gagaaatcca ccggcgttga ggcgtctatt caggtcaaac
    11041 cgtcttatgg cctgaccgat agcgaaatcg cctcgatgat caaagactca atgagctatg
    11101 ccgagcagga cgtaaaagcc cgaatgctgg cagaacaaaa agtagaagcg gcgcgtgtgc
    11161 tggaaagtct gcacggtgcg ctggctgctg atgccgcgct gttaagcgcc gcagaacgtc
    11221 aggttatcga cgatgctgcc gctcacctga gtgaagtggc gcagggcgat gatgttgacg
    11281 ccatcgaaca agcgattaaa aacgtagaca aacaaaccca ggatttcgcc gctcgccgca
    11341 tggaccagtc ggttcgtcgt gcgctgaaag gccattccgt ggacgaggtt taatatgcca
    11401 aagattgtta ttttgcctca tcaggatctc tgccctgatg gcgctgttct ggaagcgaat
    11461 agcggtgaaa ccattctcga cgttgcgctg cgtaacggta tcgagattga acacgcctgt
    11521 gaaaaatcct gtgcttgcac cacctgccac tgcatcgttc gtgaaggttt tgactcgctg
    11581 ccggaaagct cagagcagga agacgacatg ctggacaaag cctggggact ggagccggaa
    11641 agccgtttaa gctgccaggc gcgcgtcacc gacgaagatt tagtggttga aatcccgcgt
    11701 tacactatca accatgcgcg tgagcattaa cagaggttag tatgggactt aagtggaccg
    11761 atagccgcga aattggcgaa gcactgtacg atgcgtatcc cgatattgat ccgaaaacgg
    11821 ttcgattcac cgatatgcat cagtggattt gcgctctgga agatttcgac gacgacccgc
    11881 aggcatcgaa cgagaaaatc ctcgaagcga ttttgttggt ctggctggac gaagccgagt
    11941 gaatgaacgg gctgcctggg gggagcccgt tttgctgtta aacgaataag gataactaaa
    12001 atgacagaag cgatgaagat taccctctct ccccaacctg ccgacgcacg ttggggagaa
    12061 aaagcgactt acagcattaa taatgatggc attaccctgc atttgaacgg ggcagacgat
    12121 ctggggttga tccagcgtgc ggcgcgcaag attgacggtc tgggcatcaa gcatgttcag
    12181 ttaagcggtg aaggctggga tgcggatcgc tgctgggcat tctggcaagg ttacaaagcc
    12241 ccgaaaggca tacgtaaagt ggagtggccg gatctggacg atgcccagcg tcaggaactg
    12301 gataaccgcc tgatgatcat cgactgggtg cgtgacacca tcaacgcacc ggcagaagag
    12361 ctggggccat cgcaactggc acagcgtgct gttgatctga tcagcaacgt cgcaggggat
    12421 cgtgtgactt accggatcac caaaggcgaa gatctgcgtg agcaaggtta tatggggctg
    12481 cacacggtcg ggcgcggttc agaacgttct ccggtattgc tggcgctgga ttacaaccca
    12541 actggcgata aagaagcgcc agtgtacgcg tgcctggttg gtaaaggcat cacttttgat
    12601 tccggcggct acagcatcaa acagactgcg tttatggact cgatgaagtc ggacatgggc
    12661 ggcgcggcaa cggttaccgg ggcgctggca tttgccatta cgcgcggact gaacaagcgc
    12721 gtgaagctgt tcctctgttg tgcggataac ctgatcagcg gcaacgcgtt caaactgggc
    12781 gatattatta cctatcgcaa cggtaaaaaa gttgaagtga tgaacaccga tgccgaaggg
    12841 cgtctggtgc tggccgatgg cctgattgat gccagtgcgc agaaaccgga actgatcatt
    12901 gatgcggcga ccctcaccgg ggcggcgaaa accgcgctgg gtaatgatta tcacgcgctg
    12961 ttcagttttg acgatgcgct tgccggtcgc ttgttggcga gtgccgcgca ggagaatgaa
    13021 ccgttctggc gtctgccgct ggcggagttc caccgcaatc aattgccgtc taactttgcc
    13081 gaactgaaca acaccggaag cgcggcgtat ccggcaggcg cgagcacagc ggcgggcttc
    13141 ctgtcgcact ttgttgagaa ctatcagcaa ggttggctgc atatcgactg ctcggcgact
    13201 taccgtaaag cgccggttga acagtggtct gcgggtgcta cgggacttgg tgtgcgcacg
    13261 attgctaatc tgttaacggc gtaaactaat atgccggatg cggcgtaaat gccttatccg
    13321 gcctacaaag tattgcaaat tcaatatatt gcggttccct tgtaggcctg ataagcgtag
    13381 cgcatcaggc acttacgccc cgaacgttga atgaattatg gttcttatgt ccgaaacaaa
    13441 aaacgaactt gaagacctgc tggaaaaagc agcaaccgaa ccggcgcacc gcccggcctt
    13501 tttccgtact ctactggaat ccaccgtctg ggtgcctggt acggcggcac agggcgaggc
    13561 tgtggttgaa gatagcgcac ttgatttaca gcactgggaa aaagaagacg gcaccagcgt
    13621 cattcctttt ttcacctcgt tagaagcact tcaacaggcg gttgaaggcg aacaggcatt
    13681 tgtcgtaatg cctgttcgca cattgtttga gatgaccctt ggcgaaacgc tcttccttaa
    13741 tgccaaactg ccaaccggta aagaatttat gccgcgtgaa atcagtttgc tgattggtga
    13801 agaagggaat ccgcttagca gtcaggaagt actggaaggc ggtgaatcgc tgatattatc
    13861 ggaggtcgca gagccgccag cgcaaatgat tgattcactt accaccttat ttaaaaccat
    13921 taagccggtg aagcgtgttt ttatttgttc aattaaagag aacgaagagg cacagcctaa
    13981 tttacttatt ggcattgaag ccgatggtga tatcgaagaa attattcagg cggcgggaag
    14041 tgtagcgacc gatacattac ctggcgatga acccatcgat atttgtcagg tgaaaaaagg
    14101 ggaaaaaggt attagccact ttattaccga acatattgcg ccattctatg aacgtcgctg
    14161 gggtggtttt ttgcgtgact ttaaacagaa ccggataatt taaaacatgc cgccccaaag
    14221 ggcggcattc gggcttactt gctgctctga agtttgttaa ccaccagtaa attgttgccg
    14281 gagaaactcc ggttattatt tacctcggtt aacagcgcac cagaaacaat aagtacgata
    14341 caaaagcacc gtatcttcat tgtgcggtcc atgcgaagac cgcatcacga cagcaccact
    14401 ggcgatattg ccgcgatagc aagcgctgtg aaacacctgc atttacggcc atggaacatc
    14461 ctttccttaa gccagcgccc gtttgcgaga cggcggaaag ggccggtgtc gataatacgc
    14521 taaataaaca atgattactc tctttcgctt gacaaaaaga gagttactgg cgagtattgc
    14581 tttgctgtga agcacctgcg ttgctcatgc aacacaagcc aatctgctgg aaaggccgcg
    14641 aaccagacca gcaataaaaa accgccaaat tcggcggttt tttattgcct gttaaaagct
    14701 tatttctctg gctcaaccgg taaatctgct cgtgcgcccc attcactcca tgcgccgtcg
    14761 tacagtttca cgtttggcac atccagcgtc gcgagtgcca acaaaaccac ggctgctgtt
    14821 acaccagagc cgcagctgac gataatcggt ttgtcatagc tgacgccgcg accaaaaaat
    14881 atcgcatcca gttcatcggt cgtttttagt tcgccttcgc gcaccagttc cgtccacgga
    14941 acgttcaatg caccgggaat atgtccgcga cgtaaacctg ggcgaggttc atcaacttct
    15001 gcgttaaaac gtgtagccgg gcgggcatca ataatttgcg ccgtattttc atggcttgcc
    15061 aataatacat cggttacttt caccacggct tcaggattaa acgcggcgtt aaactctcct
    15121 tccggcagct ctactgcgcc ttcttctaat agcagatcat cacgctgcca gcctgcaagt
    15181 ccaccaccca gaatcgacac tttctctaca ccaaaggtgc gcagcatcca ccatgctcgt
    15241 ggggctgaga aaagattacc ttcgtcatag acaatcaggt gcttatcctg gttaacgcct
    15301 aattcacgca tcgccacggc gaacgtttcc gggcgcggca gcatgtgcgg aagcggggaa
    15361 gtgtgatcag aaagcgcttc gatatcaaaa aacactgcac cgggaatatg tccattcata
    15421 tactcctgag caacgttacg atcttcctgt ccaggcgacg ccatgcgggc atcgataatc
    15481 tgaatttccg ggtcatcaat atgttcggcg agccagtcgg ctcctacaaa ccatgtcgtg
    15541 gacataggca tctccatttt tgccggggtt aggctaaatt gtcggatgtt tagtgaaaag
    15601 cgacaagcaa aagaggctga tttggctgtc tgagccacaa ttctcactaa aattcggatt
    15661 gtatgggcgt gatactgaat tacataatgt aaattctcac gcataataat tattcgacgt
    15721 tgctaacggg ccattgtggc agggatgaaa aatgaaaaag ttacgcgtag ccgcctgcat
    15781 gctaatgctg gcgctggcag ggtgcgacaa caacgataac gcgccaacag cggtgaaaaa
    15841 agatgcgcct tctgaagtta ctaaagcggc ctcttcagaa aacgtgagtt cagcaaaact
    15901 ttccgcgccg gagcgacaaa aactggccca acagagtgcc ggtaaggcgc tgacattgct
    15961 ggatctctct gaagttcaac ttgatggcgc agccacgctg gtgctgacgt tctccattcc
    16021 tctcgacccg gatcaggatt tctcacgcgt tatccatgtc gtcgataaga aaagcggcaa
    16081 agtggatggt gcctgggagc tgtcagataa tcttaaagag ttgcgtttac gccacctcga
    16141 accgaaacgt gatttgatcg tcactattgg caaggaggtc aaagcactca acaacgcaac
    16201 cttcagtaaa gattacgaaa aaactataac tacccgtgat atccaaccca gcgtcggttt
    16261 tgccagccgt ggttcgttgt tacctggtaa agttattgaa gggttgccgg taatggcgct
    16321 caacgttaac aatgtcgatg ttaacttctt ccgtgtgaaa ccagaatcac tgcccgcttt
    16381 cattagccaa tgggaatacc gcaattcgct ggcgaactgg cagtcagaca aactgctgca
    16441 gatggcggat ctggtctaca ctggacggtt tgatctcaat cctgcgcgta acacccgtga
    16501 aaaattattg ctgccgctgg gcgatatcaa accgcttcag caggcaggcg tgtatctggc
    16561 tgtgatgaat caggctggac gttacgatta cagtaatccc gcgacgctgt ttacgttaag
    16621 tgatatcggc gtttcagctc accgttatca caatcgtctg gatatcttta cccaaagtct
    16681 ggaaaacggc gcggcccagc aaggaataga agtctcttta ttaaatgaga aagggcagac
    16741 tctgactcag gcaaccagtg atgctcaggg gcatgtgcag ctggaaaatg ataaaaacgc
    16801 ggcattactg ctggcgcgta aagacggtca gacaacgcta ctcgatttaa aactcccggc
    16861 gctggactta gcggaattta acattgctgg cgcgccgggc tatagcaaac agtttttcat
    16921 gtttggcccg cgcgatcttt atcgcccggg tgaaacggtc atcctcaatg gtttgctgcg
    16981 tgatgcagac ggtaaagcat tgcccgatca acccatcaag ttagacgtga ttaaacccga
    17041 cgggcaggta ctcaggagcg tcgttagtca gccggagaat ggcctctacc actttacctg
    17101 gccactcgat agcaatgcgg caaccggtat gtggcatatt cgcgctaaca cgggcgataa
    17161 ccagtaccgg atgtgggatt tccacgtcga agattttatg ccagagcgca tggcgctgaa
    17221 tctgaccggt gagaaaaccc cgctaacgcc gaaagatgaa gtgaaattct ccgtggtggg
    17281 gtactacctg tatggtgcgc ctgctaatgg taatattttg caagggcaac ttttcctgcg
    17341 cccactgcgt gaagctgtgt cagccttacc tggttttgaa ttcggagata tagctgccga
    17401 aaatctttcc cgcacgctgg atgaagttca gttgacgctg gatgataaag ggcgcggcga
    17461 agtttctaca gaaagccagt ggaaggaaac gcattcccca ttacaggtta ttttccaggg
    17521 tagtttgctg gaatcgggcg gtcgcccggt gacgcgccgt gctgagcagg ctatctggcc
    17581 tgccgatgca ttgccgggga tccgtccgca gttcgcctcg aaatcggttt acgattatcg
    17641 caccgacagc acggtgaaac agcccattgt tgatgaaggc agtaacgccg cttttgacat
    17701 cgtttatagc gatgcgcaag gcgtgaaaaa agccgtgtcg ggcttgcagg tgcgcctgat
    17761 tcgcgaacgc cgcgattact actggaactg gtcggaatat gaaggctggc agtcacagtt
    17821 tgatcaaaaa gatctgatcg aaaatgaaca aactctggat ctgaaagcgg acgaaaccgg
    17881 taaggtcagt ttcccggtag agtggggcgc ttatcgtctg gaagttaaag cgccgaatga
    17941 agcggtcagt agtgtgcgtt tctgggctgg ttatagctgg caggataaca gcgacggggg
    18001 tggtgcagtg cgacccgacc gtgtcacgct gaaactggat aaagccagct atcgccctgg
    18061 cgataccatt aagttgcata ttgccgcgcc aacggcgggt aaaggttatg cgatggtcga
    18121 gtccagtgaa gggccgctgt ggtggcaaga gattgatgtt ccggctcaag ggctggatct
    18181 gacgattccg gtcgataaaa cctggaatcg tcatgatctg tatttaagta cgctggtggt
    18241 acgtcctggc gataaatctc gctccgcgac gccaaaacgc gcggttgggg tcttgcatct
    18301 gccgcttggt gatgaaaacc gtcgcctcaa tctggcgctg gaaaccccca ccaaaatgcg
    18361 ccccaatcag ccattaaccg tgaaaattaa agccagcaat aaaaatggtg agatgccaaa
    18421 acaggtgaat gtgctggtgt ctgccgttga tagcggtgtg ctgaatatta ctgattatgt
    18481 cacgccagat ccgtggcagg cgttctttgg tcagaaacgc tatggcgcag acatttacga
    18541 tatttacggt caggtcattg aaggtcaggg gcgtctggca gctctgcgtt ttggtggtga
    18601 tggtgatgag ctgaaacgtg gtggtaaacc gccggtcaat cacgtcaata ttgtcgcgca
    18661 gcaggcgctg ccggtaacgc tcaatgaaca gggcgaaggc tcggttacac tgccaattgg
    18721 cgattttaac ggtgaattgc gcgtcatggc gcaagcctgg acggcagatg acttcggaag
    18781 caacgaaagc aaagtgattg ttgccgcacc ggtgattgct gaactgaaca tgccgcgctt
    18841 tatggcgagt ggcgatacct cgcgcctgac gctggatatc actaatctta ccgataaacc
    18901 gcaaaaactt aatgttgctc tgaccgccag tggcttgctt gaactggtga gtaattcccc
    18961 cgcacctgtt gaattagcgc caggggtgcg cactacgctg tttatcccgg tgcgtgcatt
    19021 gccgggttat ggcgatggag acattcaggc caccattagc gggttagcgt taccggggga
    19081 aaccgttgcc gatcagcata agcagtggaa aatcggcgtc cgtccggcat tcccggcaca
    19141 aacggtcaat tacggtacgg cgttacagcc cggtgaaacc tgggcgctcc cggctgatgg
    19201 attgcaaaac ttctcgcctg ttacgctgga agggcaattg ttgttgagcg gcaaaccacc
    19261 gctgaacatc gcacgttata tcaaagagtt aaaagcgtat ccgtacggct gtctcgagca
    19321 aaccgccagc ggcctgttcc cgtcacttta taccaatgca gcccaactgc aggcgttggg
    19381 catcaaaggc gacagtgatg agaaacgccg tgcatcggtc gatatcggca tttcccgttt
    19441 gctgcaaatg caacgtgata acggcggctt tgcgctgtgg gataaaaacg gtgacgaaga
    19501 gtactggctg acggcttacg tgatggattt cctggtccgc gcaggcgaac agggttacag
    19561 cgtgccgaca gacgccatta accggggtaa tgagcgtctg ctgcgctatt tacaagatcc
    19621 gggcatgata tcgatcccgt acgctgataa tctcaaagcc agtaaattcg ccgtacagtc
    19681 ttacgctgcg ctggtgttgg cccgtcagca aaaagcacca ctgggtgcgc tgcgtgaaat
    19741 ctgggagcat cgtgcagatg ccgcttctgg tttaccgctg ctgcaacttg gcgttgcgct
    19801 gaaaaccatg ggtgatgcaa tgcgtggtga agaagcgatt gtgctggcgc tgaaaacgcc
    19861 gcgtaatagt gatgagcgga tatggctggg tgattacggt agtccactgc gcgacagcgc
    19921 gttgatgctc tcattgctgg aagagaacaa actgctaccc gatgagcagt actccctgtt
    19981 gaacacactt tcgcagcagg cgttcggtga acgctggcta tcgacgcagg aaagtaacgc
    20041 gttgttcctg gctgcccgta ctcttcagga tttaccgggc aaatggcagg cgcaaaccac
    20101 tttctctgct gagccgctga caggcgagaa agcgcaaacc agcaatctga atagcgatca
    20161 acttgccacc ttgcaggtga ccaacagtgg cgatcagccg ttatggctgc gtgtggatgc
    20221 cagcggttat ccgcaatccg cacctttatc ggcgagcaat gtgctgcaaa ttgagcgtca
    20281 tattctgggt actgacggta agagcaaatc gctggactcg ttacgtagcg gcgatctggt
    20341 gctggtgtgg ttgcaggtta aagccagtaa cagtgtaccg gatgcgttag tcgtggatct
    20401 gttgcctgcg gggctggaac tggaaaacca gaatctggcg aacggcagcg ccagcctaga
    20461 gcaaagtggt ggtgaagtgc agaacttgct gaaccagatg cagcaggcga gcattaagca
    20521 catagagttc cgtgacgatc gctttgtggc ggcggttgcc gttgatgaat atcaaccggt
    20581 aacgctggtg tatctggcgc gggcggtgac gccgggaacg tatcaggtgc cgcagcctat
    20641 ggtggaatca atgtatgttc ctcaatggcg ggcgactggt gcggctgacg atctgttgat
    20701 agtcagaccg taaatgcctc gcctgttaac caaacgcggc tgctggataa tgctggcagc
    20761 cgcgcctttt atcatcattc tggcagcatg ggcagcagat aaactctggc ctctgccatt
    20821 gcaagaagtg aatcctgcac gcgtggtggt ggcgcaggat ggtacgccac tctggcgttt
    20881 cgccgatgct gacggtatct ggcgttatcc ggtaacaatc gaagatgttt ctccacgtta
    20941 ccttgaagcg ctgatcaatt atgaagatcg ctggttctgg atgcatccgg gggtgaatcc
    21001 gttctcggtg gcgcgtgcag cctggcaaga tctcacgtcg ggtcgggtca tttccggcgg
    21061 cagcacgctc accatgcagg tggcgcgcct gcttgatcct caccccaaaa cgtttggcgg
    21121 caaaattcgc cagctctggc gcgcgttgca actggaatgg catctgtcta aacgtgaaat
    21181 tctgaccttg tatcttaacc gtgccccgtt tggcggtacg ttgcagggga ttggtgcggc
    21241 aagttgggct tacctcggga aatcgcctgc gaatttaagc tattccgagg cggcaatgct
    21301 ggcggtgttg ccgcaagcgc ccagccgtct tcgcccggat cgttggccgg agcgtgccga
    21361 agccgcgcgt aataaagtgc tcgaacggat ggccgtgcaa ggtgtgtggt cccatgagcg
    21421 ggtaaaagag tcaagggaag aacccatctg gctggcaccc cgacaaatgc cgcaactggc
    21481 accgctgttt tcgcgcatga tgctcggtaa aagcaaaagc gacaaaatcg ttactacgct
    21541 ggatgccggt cttcaacgac gtctggaaga actggcgcaa aactggaaag gacgattacc
    21601 accgcgcagc tcactggcga tgatcgtggt tgatcatacc gatatgcgtg ttcgcggctg
    21661 ggtgggttcg gttgatctca acgatgattc acgctttggt catgttgata tggtcaatgc
    21721 gatccgatcg ccaggatcgg tgctcaaacc gtttgtttac ggtctggcgc tggatgaagg
    21781 cttgatccac ccggcatcgc tgttgcaaga cgtcccccgg cgtaccggtg attaccgacc
    21841 tggcaacttt gacagcggtt ttcatgggcc gatcagcatg agcgaggcgc tggtgcgctc
    21901 gctgaattta ccagctgtgc aggtgctgga agcctatgga ccgaaacggt ttgcggcaaa
    21961 attacgcaat gttggattgc cgttgtatct gcccaacggt gctgcgccga atctttcgct
    22021 cattctcggc ggcgctggtg caaaactgga agatatggcg gcggcctata ccgcgtttgc
    22081 tcgccacggc aaggcaggta aattgcgctt acagcctgac gatccgctgc ttgaacggcc
    22141 tttaatgtcg tcgggggctg cgtggatcat tcgccggatt atggctgatg aagcgcaacc
    22201 cttgccggat ggtgccttgc cgcgcgtcgc cccactggca tggaaaacgg gcaccagcta
    22261 tggctatcgt gatgcctggg cgattggggt taacgctcgc tatgtcattg ggatctggac
    22321 tggcagaccg gacggcacgc ccgttgttgg tcagtttggc tttgccagtg ccgtaccgct
    22381 gttgaatcag gttaataata tcttactgtc gcgcagtgtg aatttgccgg aagacccacg
    22441 tcctgactct gtaagtcgtg gcgttatctg ctggccgggc ggacagtctt tgccggaagg
    22501 tgacggtaac tgtcgccgcc gcctggcaac ctggctgctg gacgggagcc agccgccaac
    22561 tctattactg ccggagcagg aaggcattaa tggcattcgt ttccccatct ggctggatga
    22621 aaacggcaaa cgtgttgccg ctgattgccc tcaggcgcga caagaaatga taaatgtctg
    22681 gccgctaccg ctggaaccct ggctgcccgc atcagaacgt cgcgctgtgc gcttgccacc
    22741 ggcctcgaca atttgcccgc catacggtca cgatgcacaa ctcccgctgc agctaacagg
    22801 tgtgcgcgat ggcgcgatta ttaaacgttt accgggcgca gcggaagcga ctttgccgtt
    22861 gcaatcaagt ggaggggcag gtgaacgctg gtggtttctg aatggcgaac cgttaactga
    22921 acgtgggcgc aacgtgaccc tgcatttgac ggataaaggc gattatcaat tgctggtgat
    22981 ggatgacgtg gggcaaatag cgacagtgaa atttgtcatg caatagtctg ttttgtccct
    23041 gattgttgct taaactgggt ttattttaaa aaaatgttac cttcatcaat agtcaacggc
    23101 cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca acagaacaat
    23161 ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg aacgcggtag
    23221 caaaaaacgt gattggtagt atctttgcgc gctttgaagc tgcagggttc aaaattgtcg
    23281 gcaccaaaat gctgcacctg accgttgaac aggctcgtgg cttttatgct gaacacgatg
    23341 gaaaaccgtt ctttgatggt ctggttgaat tcatgacttc tggcccgatc gttgtttccg
    23401 tgctggaagg tgaaaatgct gttcagcgtc accgcgatct gctgggcgcg accaatccgg
    23461 caaacgcact ggccggtact ctgcgtgctg attacgctga cagcctgacc gaaaatggta
    23521 cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc ttcggcgaag
    23581 gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa acgtggcatc
    23641 cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca ccggggcgtt
    23701 tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat agcatatgtc
    23761 tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca acctgctgga
    23821 tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaac ccttccgcgc
    23881 cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg agatgaccga
    23941 catcaataaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgtg caccggaagt
    24001 ggttgaagaa cagcgttcat ctgacggcac cattaaatgg gcgatcgccg ttggcgatca
    24061 gcgcgttgaa acggtatata tcccggaaga cgaccgtgcc acgctctgcg tctcttcgca
    24121 ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct ttaaccgcaa
    24181 cctgcgggta tcggaaatta tcggccaggt gtggcgtgcg gcgaaaattg tcggcgcagc
    24241 aaaagtcacc ggtcagcgcc caatcactaa cgtagtgatg atgggtatgg gcgagccgct
    24301 gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt tcggttttgg
    24361 cctgtcaaaa cgtcgcgtca cgctttccac ttccggtgtg gttccggcgc tggataaact
    24421 gggcgatatg atcgacgtag cgctggcaat ttccctgcac gcgccgaacg acgaaattcg
    24481 tgacgagatt gtgccgatca acaaaaagta caatatcgaa acgttccttg cggcggtgcg
    24541 ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat acgtgatgct
    24601 tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactac tgaaagatac
    24661 gccgtgtaag atcaacctga tcccgtggaa cccgttcccg ggcgcgccgt atggacgcag
    24721 ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt tcaccactat
    24781 tgtgcgtaaa actcgtggtg atgatatcga tgctgcttgt ggtcagttgg cgggcgatgt
    24841 tatcgaccgt acgaaacgta ctctgcgtaa gcggatgcag ggtgaagcca tcgacattaa
    24901 agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa tcttttatga
    24961 atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg ctgactttaa
    25021 ggcagtatgt aacgatgcaa cagtaagtta gccttagcga atgtgggctt tgtcacgagc
    25081 acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc gcggtgtggg
    25141 tgggcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca gcgaatgaat
    25201 actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct gcgtaatgct
    25261 cgcgaacaac taggacttag tcaacaggcc gttgccgagc gactttgcct gaaggtttcc
    25321 acggtacgcg acattgaaga agataaggca cctgccgatc ttgcttcaac attcctgcgc
    25381 ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact gctgccaggg
    25441 ctggaaaagc aggctccact acgggctgca aaagttgcgc cgatgcagag tttttccctc
    25501 ggtaaacgcc gcaaaaaacg tgacggctgg ctgatgacct tcacctggct ggtgttgttt
    25561 gtggttattg gtctgagcgg tgcctggtgg tggcaagacc acaaagctca gcaggaagag
    25621 atcaccacta tggccgatca atcttctgcg gaactgaata ataatcaaag ccagagtgta
    25681 ccgttagata cgtcgacaac cacagatcag gctatggcga ccacgccgac atcacctgtg
    25741 gataccaccg caaccaacac gcaaacacct gccgtaactg cgccagcacc agctgttgat
    25801 ccgcaacaga atgcggttgt tccaccttcg caggcaaatg ttgataccgc tgcgactccg
    25861 gcaccagcgg caacaacaac accagatggc gctgcgccgt tgccaaccga tcaggctggc
    25921 gtgaccacgc cggcggttga cccgaatgcg ctggtgatga actttactgc cgattgctgg
    25981 ctggaagtca ccgatgctac cggtaaaaaa ctgtttagcg gtatgcagcg taaagacggt
    26041 aatttgaact taaccggcca ggcaccgtac aaactgaaaa ttggtgcgcc agccgcagta
    26101 cagatccagt atcaagggaa acctgtcgat ctgagtcgtt ttatcagaac taaccaggtt
    26161 gcgcgtctga ccctcaatgc cgaacaatca ccggcgcagt aacagacggg taacgcggga
    26221 gatttttcat gcataaccag gctccaattc aacgtagaaa atcaacacgt atttacgttg
    26281 ggaatgtgcc gattggcgat ggtgctccca tcgccgtaca gtccatgacc aatacgcgta
    26341 cgacagacgt cgaagcaacg gtcaatcaaa tcaaggcgct ggaacgtgtt ggcgctgata
    26401 tcgtccgtgt ctccgtaccg acgatggacg cggcagaagc gttcaaactc atcaaacagc
    26461 aggttaacgt gccgctggtg gctgacatcc acttcgacta tcgcattgcg ctgaaagtag
    26521 cggaatacgg cgtcgattgt ctgcgtatta accctggcaa tatcggtaat gaagagcgta
    26581 ttcgcatggt ggttgactgt gcgcgcgata aaaacattcc gatccgtatt ggcgttaatg
    26641 ccggatcgtt ggaaaaagat ctgcaagaaa agtatggcga accgacgccg caggcgttgc
    26701 tggaatctgc catgcgtcat gttgatcatc tcgatcgcct gaacttcgat cagttcaaag
    26761 tcagcgtgaa agcgtctgac gtcttcctcg ctgttgagtc ttatcgtttg ctggcaaaac
    26821 aaatcgatca gccgctgcat ctggggatca ccgaagccgg tggtgcgcgt agcggggcag
    26881 taaaatccgc tattggttta ggtctgctgc tgtctgaagg catcggcgac acgctgcgcg
    26941 tatcgctggc ggctgatccg gtcgaagaga tcaaagtcgg tttcgatatt ttgaaatcgc
    27001 tgcgtatccg ttcgcgcggg atcaacttca tcgcctgccc gacctgttcg cgtcaggaat
    27061 ttgatgttat cggtacggtt aacgcgctgg agcaacgcct ggaagatatc atcactccga
    27121 tggacgtttc gattatcggc tgcgtggtga atggccctgg tgaggcgctg gtttctacac
    27181 tcggcgtcac cggcggcaac aagaaaagcg gcctctatga agatggcgtg cgcaaagacc
    27241 gtctggacaa caacgatatg atcgatcagc tggaagcgcg cattcgtgcg aaagccagtc
    27301 agctggacga agcgcgtcga attgacgttc agcaggttga aaaataataa cgtgatggga
    27361 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
    27421 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
    27481 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
    27541 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
    27601 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
    27661 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
    27721 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
    27781 cgggcctatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
    27841 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gccgaactga ttatgctcac
    27901 tgcccgctgg tggcgcgcgc tgggtatttc cgagcacgta actcttgagc tgaactctat
    27961 cggttcgctg gaagctcgtg cgaattaccg cgatgcgctg gtggcattcc ttgagcagca
    28021 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgt tgcgcgtact
    28081 cgattcaaaa aatccggaag tgcaggtgct tctcaacgac gctccggcat taggcgatta
    28141 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgcttg agagcgcggg
    28201 gatcgcttac accgtcaacc agcgtctggt gcgcggtctg gattactata accgtaccgt
    28261 tttcgagtgg gtgactaaca gcctcggttc ccagggcacc gtgtgtgcag gtggtcgtta
    28321 tgacggtctg gtggaacaac tgggtggtcg tgcaacaccg gcagtcggtt ttgcgatggg
    28381 cctcgaacgt cttgtattgt tagttcaggc cgttaatccg gaatttaaag ccgatcctgt
    28441 tgtcgatata tacctggtgg cttcaggtgc tgacacacaa tctgcggcta tggcattagc
    28501 agagcgtctg cgtgatgaat taccgggcgt gaaactgatg accaaccacg gcggcggcaa
    28561 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
    28621 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
    28681 tgcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
    28741 aaggacagcg tggaaattta cgagaacgaa aacgaccagg ttgaagcggt taaacgcttt
    28801 tttgctgaaa atggcaaagc actggctgtt ggggtgattt tgggcgttgg cgcactgatt
    28861 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
    28921 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccagc ggcggaaaaa
    28981 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
    29041 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
    29101 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
    29161 ctcaagcagg ctgatgccgc gttaaaaacc cttgatacca tcaaaggtga agggtgggct
    29221 gccattgttg ccgacctgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
    29281 cgtagtgcat gggaagcagg cgtgaaaagc gatgttaccc cggcactgag cgaaatgatg
    29341 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
    29401 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
    29461 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgactactgt
    29521 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
    29581 ggcggacaac gttgtctatg cagcggaccg cgctggttta gtaaaagcgc tgaatgcgga
    29641 tgacggcaaa gaaatctggt ctgtcaacct ggccgagaaa gatggctggt tctctaaaga
    29701 tcctgcatta ctttctggcg gcgtgaccgt gtctggtggg catgtctaca ttggcaccga
    29761 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
    29821 cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag
    29881 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
    29941 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
    30001 cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
    30061 ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
    30121 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
    30181 gacggcgctt gatctgcgca gcggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
    30241 tgatttcatc gtcgacggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
    30301 gttgaccatt gatggcggcg ttacgctgtg gacacaaagc gatctgctgc atcgcctgct
    30361 gacttctccg gtgctgtata atggcaacct ggtggtaggc gacagtgaag gttatctgca
    30421 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
    30481 ccagactgaa ccggttgctg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
    30541 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
    30601 gccgttttcc tgtttttaac aacgacacga atatagcgtg cgttgtctga tgatttataa
    30661 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
    30721 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
    30781 tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgcgagt ttatctgcat
    30841 tgataccggc gggattgatg gcaccgaaga cggtgtagag acacgtatgg cggaacagtc
    30901 gctgctggcg attgaagaag cggacgttgt actgtttatg gtggatgcgc gcgcgggcct
    30961 gatgccggcg gatgaagcga ttgctaaaca tctgcgctcc cgtgaaaaac cgaccttcct
    31021 ggtggcaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctacgcgct
    31081 tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
    31141 ggagcacgtg ctgctgccgt ggatggaaga tctcgcaccg caagaagaag tcgacgaaga
    31201 cgccgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggcagacga
    31261 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
    31321 gtctacactc actaaccgta ttcttggtga agagcgcgtt gttgtttacg acatgcctgg
    31381 cacgacgcgt gacagtatct acattccaat ggaacgcgac ggacgtgagt atgtgctcat
    31441 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gcggtagaga aattctcggt
    31501 aatcaaaacg ttgcaggcca ttgaagatgc caacgtggtg atgttggtga ttgatgcgcg
    31561 tgaaggtatt tccgatcagg atctctcctt gctgggcttt attctcaata gtgggcgctc
    31621 acttgtgatt gtagtgaaca agtgggacgg cctgagccag gaagtgaaag agcaggtgaa
    31681 agagacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
    31741 cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc
    31801 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    31861 ccaaccaccg ctggtacgcg gtcgtcgtgt gaagctgaaa tacgcccacg ccgggggtta
    31921 taacccgccg attgtggtga ttcacggcaa tcaggtgaaa gacctgcctg attcctacaa
    31981 gcgttacctg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    32041 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    32101 gatgcgtaaa cgtaagcgtc tgatgaagca tatcaagaaa agtaagtaat cacttatctg
    32161 gtttcccttt ccacacggag agggaagccg aataacacgc gaggagtatt tatggcactc
    32221 aactgtccaa cctgcaaaac ccctctcgaa cgcaatggcg agactgctca ctgtgcaact
    32281 tgcgacaaag actttaccgt ccaggcactt tgccctgagt gtcatcaggc gttacaagtg
    32341 ttaaaagctt gtggtgctgt ggattatttc tgccaacatg ggcacggttt aatatctaaa
    32401 aaacgcgttg aatttgttat tgcttaatat catgtgaata tattgtttta tcattt
//
LOCUS       DADTFG010000041        32271 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.041, whole genome shotgun sequence.
ACCESSION   DADTFG010000041 DADTFG010000000
VERSION     DADTFG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.041"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            223..1281
                     /gene="citC"
                     /locus_tag="J1138_001770"
     CDS             223..1281
                     /gene="citC"
                     /locus_tag="J1138_001770"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBA8453578.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1296..1592
                     /gene="citD"
                     /locus_tag="J1138_001771"
     CDS             1296..1592
                     /gene="citD"
                     /locus_tag="J1138_001771"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBA8453579.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILEVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1589..2497
                     /gene="citE"
                     /locus_tag="J1138_001772"
     CDS             1589..2497
                     /gene="citE"
                     /locus_tag="J1138_001772"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBA8453580.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2508..4040
                     /gene="citF"
                     /locus_tag="J1138_001773"
     CDS             2508..4040
                     /gene="citF"
                     /locus_tag="J1138_001773"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBA8453581.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4044..4595
                     /gene="citX"
                     /locus_tag="J1138_001774"
     CDS             4044..4595
                     /gene="citX"
                     /locus_tag="J1138_001774"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBA8453582.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4570..5448
                     /gene="citG"
                     /locus_tag="J1138_001775"
     CDS             4570..5448
                     /gene="citG"
                     /locus_tag="J1138_001775"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBA8453583.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSVTPITICATAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNISP
                     GGSADLLILTWFLAQI"
     gene            5499..6962
                     /gene="citT"
                     /locus_tag="J1138_001776"
     CDS             5499..6962
                     /gene="citT"
                     /locus_tag="J1138_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBA8453584.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7076..7882
                     /gene="rna"
                     /locus_tag="J1138_001777"
     CDS             7076..7882
                     /gene="rna"
                     /locus_tag="J1138_001777"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBA8453585.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8112..8522
                     /gene="rnk"
                     /locus_tag="J1138_001778"
     CDS             8112..8522
                     /gene="rnk"
                     /locus_tag="J1138_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBA8453586.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8879..9307
                     /gene="uspG"
                     /locus_tag="J1138_001779"
     CDS             8879..9307
                     /gene="uspG"
                     /locus_tag="J1138_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBA8453587.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9427..10992)
                     /gene="ahpF"
                     /locus_tag="J1138_001780"
     CDS             complement(9427..10992)
                     /gene="ahpF"
                     /locus_tag="J1138_001780"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBA8453588.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11163..11726)
                     /gene="ahpC"
                     /locus_tag="J1138_001781"
     CDS             complement(11163..11726)
                     /gene="ahpC"
                     /locus_tag="J1138_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBA8453589.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12098..12844
                     /gene="dsbG"
                     /locus_tag="J1138_001782"
     CDS             12098..12844
                     /gene="dsbG"
                     /locus_tag="J1138_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBA8453590.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            13049..13951
                     /locus_tag="J1138_001783"
     CDS             13049..13951
                     /locus_tag="J1138_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8453591.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FMNRKVSFRSSSLMTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            14098..15318
                     /locus_tag="J1138_001784"
     CDS             14098..15318
                     /locus_tag="J1138_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBA8453592.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            15291..15920
                     /locus_tag="J1138_001785"
     CDS             15291..15920
                     /locus_tag="J1138_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBA8453593.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(15921..17081)
                     /gene="ybdL"
                     /locus_tag="J1138_001786"
     CDS             complement(15921..17081)
                     /gene="ybdL"
                     /locus_tag="J1138_001786"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBA8453594.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLHAEPEHYLALPDFYRQKRDILVNALSESRLEILACEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            17190..18278
                     /gene="hcxA"
                     /locus_tag="J1138_001787"
     CDS             17190..18278
                     /gene="hcxA"
                     /locus_tag="J1138_001787"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBA8453595.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(18288..18485)
                     /locus_tag="J1138_001788"
     CDS             complement(18288..18485)
                     /locus_tag="J1138_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBA8453596.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYGNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(18598..20703)
                     /gene="cstA"
                     /locus_tag="J1138_001789"
     CDS             complement(18598..20703)
                     /gene="cstA"
                     /locus_tag="J1138_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBA8453597.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(20884..21297)
                     /gene="entH"
                     /locus_tag="J1138_001790"
     CDS             complement(20884..21297)
                     /gene="entH"
                     /locus_tag="J1138_001790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBA8453598.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(21300..22046)
                     /gene="dhbA"
                     /locus_tag="J1138_001791"
     CDS             complement(21300..22046)
                     /gene="dhbA"
                     /locus_tag="J1138_001791"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HBA8453599.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(22046..22903)
                     /gene="entB"
                     /locus_tag="J1138_001792"
     CDS             complement(22046..22903)
                     /gene="entB"
                     /locus_tag="J1138_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBA8453600.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAEL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(22917..24527)
                     /gene="entE"
                     /locus_tag="J1138_001793"
     CDS             complement(22917..24527)
                     /gene="entE"
                     /locus_tag="J1138_001793"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HBA8453601.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHATSDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQLNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(24537..25712)
                     /gene="entC"
                     /locus_tag="J1138_001794"
     CDS             complement(24537..25712)
                     /gene="entC"
                     /locus_tag="J1138_001794"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBA8453602.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            25901..26857
                     /gene="fepB"
                     /locus_tag="J1138_001795"
     CDS             25901..26857
                     /gene="fepB"
                     /locus_tag="J1138_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA8453603.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVATQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(26861..28111)
                     /gene="entS"
                     /locus_tag="J1138_001796"
     CDS             complement(26861..28111)
                     /gene="entS"
                     /locus_tag="J1138_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBA8453604.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDN"
     gene            28222..29226
                     /gene="fepD"
                     /locus_tag="J1138_001797"
     CDS             28222..29226
                     /gene="fepD"
                     /locus_tag="J1138_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBA8453605.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLTTPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            29223..30215
                     /gene="fepG"
                     /locus_tag="J1138_001798"
     CDS             29223..30215
                     /gene="fepG"
                     /locus_tag="J1138_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBA8453606.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDTPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            30212..31027
                     /gene="fepC"
                     /locus_tag="J1138_001799"
     CDS             30212..31027
                     /gene="fepC"
                     /locus_tag="J1138_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA8453607.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(31024..32157)
                     /gene="fepE"
                     /locus_tag="J1138_001800"
     CDS             complement(31024..32157)
                     /gene="fepE"
                     /locus_tag="J1138_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBA8453608.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLSLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDINIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLRPSLPVKKD
                     GPGKSIIVILSALIGGMVACGGVLLRHAMASRKQDAMMADHLV"
BASE COUNT         7769 a         8788 c         8116 g         7598 t
ORIGIN      
        1 aaacttaaaa ccattaaata aataaaaaac cactaactct atgtgaaata aatcaaaatt
       61 tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata cctcgatcgc
      121 ccccctttct cccaagtgaa aataaaaggt tatcaatttg caacattgaa caacattcgt
      181 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      241 ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      301 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      361 aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc tatcagtgaa
      421 tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa cctcgcctat
      481 gagcggcata gcacgcatct gtttatttat accaaaaccg aatacgaggc gctgttccgc
      541 cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat ggaaaacagc
      601 gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc agggaacaag
      661 attggctgca ttgtgatgaa cgccaatccc tttacgaatg gtcaccgtta tctgattcaa
      721 caggctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc
      781 ttcccctatg aagaccggct ggatctggtg ttaaaaggca ccgccgatat tccacgcctg
      841 actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg ctacttcatt
      901 aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat tttccgtcag
      961 tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc
     1021 gttactgccc agtacaacca ggatatgcgc tactggctgg aaacaccgac tatctccgca
     1081 ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct
     1141 tcccgggtac gtcaactgct ggcaaaaaac gatctcacgg ctatcgcgcc gctggtccct
     1201 gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct
     1261 cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg
     1321 caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata
     1381 tcgacctgca aatcaacagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca
     1441 ttctggaagt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag
     1501 gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc gccagcggta
     1561 tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac
     1621 tcgcacccgc cgcagtatgt tgtttgtgcc tggtgctaat gccgcgatgg tcagcaactc
     1681 cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgttg cattgcgtga
     1741 aaaagacacc gcccgccgca tggtttacca cgcgctgcaa catccgcttt atcgcgatat
     1801 tgaaaccatt gtgcgtgtaa acgcgctgga ttccgaatgg ggtgttaacg acctggaagc
     1861 cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt
     1921 tctggatatc gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag
     1981 taccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcgg tagaaatcgc
     2041 tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg tgcgcaacct
     2101 gcgtacagaa cgctccccgg aaggaactga actgctgttc gctcgctgct ccattttgca
     2161 ggccgcgcgt tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga
     2221 agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg gcaaatcgct
     2281 gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc agaaagaagt
     2341 ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt
     2401 ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct
     2461 ctcccgcgca gaactttccg gcatccgcga agaataaggc aatcaaaatg acgcagaaaa
     2521 ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc
     2581 ttgccgcttt ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca
     2641 aactgtgcgc caacctggaa gaagcgattc gtcgctctgg tttacaggac ggcatgacgg
     2701 tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca
     2761 tcgcgaagat gggctttaaa aacctgactc tggcctccag ctccctgagt gattgccatg
     2821 cgccactggt agaacacatt cgccagggcg tggttacccg catttatacc tctggcctgc
     2881 gcggtccact ggcggaagaa atctcccgtg gtctgctggc ggaaccggtg caaatccact
     2941 ctcacggcgg acgtgtgcat ctggtacaga gcggcgaact gaatatcgat gtggctttcc
     3001 tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctactccggt aaagcctgct
     3061 gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc gtgatgctta
     3121 ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatc
     3181 tgatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt gctacccgta
     3241 tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgttaact
     3301 ctggctactt caaagaaggt ttctccatgc agaccggcac cggcggcgct tcgctggcgg
     3361 taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg
     3421 gcggtattac cgcaacgatg gttgatctgc acgaaaaagg tttgatccgc aaactgctgg
     3481 atgtgcagag ctttgacagc catgctgcgc aatcgctggc ccgtaacccc aatcacatcg
     3541 aaatcagcgc caaccagtac gctaactggg ggtcgaaagg cgcagcggtt gatcgtctcg
     3601 acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac gtgctgaccg
     3661 gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg attgcttctg
     3721 cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgacgctg gtggataacg
     3781 tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac cacggcatcg
     3841 ctgttaaccc ggcacgtccg gagctggcag aacgtctgca ggaagcgggg atcaaagtgg
     3901 tttctattga gtggctgcgt gaacgtgcgc gtctgctgac cggtgaacca cagccgattg
     3961 aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg
     4021 tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg
     4081 tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgcctggc
     4141 tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag
     4201 acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt gccttagccg
     4261 caaaacaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg
     4321 gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac
     4381 atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa ggcgacattc
     4441 tctcccgccg cgattattca ctgccgcctc gccgctgcct gttgtgcgag caaagcgcag
     4501 ctgtttgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcgc
     4561 tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc gacgtcatta
     4621 atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa cctgtcaccg
     4681 aaaccaggcc tcgtggatcg cattaactgc ggtgcgcaca aagatatggc gctggaagat
     4741 ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga atttggtgcc
     4801 tgtagtgcag aaatggcacc ggaagcggta ctcaacggat tacgcccaat tggtatggct
     4861 tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg cagcattttt
     4921 tctttagggc tactgtgtgc ggcaattggc cgtttgcttc aactcaacca gtcggtaacg
     4981 ccaataacaa tttgtgcgac ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg
     5041 cgtaccaata attcacaact gacggcaggt caacggttgt accagcagct tggccttacc
     5101 ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaaccacgc cttgccgcat
     5161 tacctcactc tgctggatca ggggttagat cctgagctgg cattgctcga taccttgctc
     5221 ctactgatgg ctaccaacgg cgataccaac gttgcatcgc gcggtggcga ggggggcctg
     5281 cgctggctac agcgcgaggc gcaaacatta ttaaacaatg ggggcatccg aacccccgcc
     5341 gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatat cagtccaggc
     5401 ggcagtgccg acctgctgat tcttacctgg tttttagcac agatttaatt atttaagcac
     5461 ttgataaatt tggaaatatt aattttcgga gaacccgtat gtctttagca aaagataata
     5521 tatggaaatt attggcgccc ctggtggtga tgggtgtcat gtttcttatc cctgtacctg
     5581 acggtatgcc gccgcaggcg tggcattact ttgcagtgtt tgtggcaatg attgtcggca
     5641 tgatcctcga gccaattccg gcaacggcga tcagttttat tgcggttact atttgcgtta
     5701 ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg tttaatgcgc
     5761 aaaaacaggc gctgaaatgg ggtctggctg gtttttccag caccacggta tggctggtat
     5821 ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt cgcattgccc
     5881 ttttcctggt gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg attgtcatta
     5941 tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg ggtacggttt
     6001 ttccggtcat taaaaacctg ccgccgctgt ttaaatcatt cccgaacgat ccgtccgcgc
     6061 gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg agttcgtcca
     6121 tgtttgtcac cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc aaaattgccg
     6181 gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg gttatcttgc
     6241 ttatcattgc gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca cacagtgaag
     6301 aagtggcaac ctgggcgggt gatgaactaa aaaccatggg tgcgctgacg cgcagagagt
     6361 ggacgctgat tggccttgta ttgctcagct taggtttgtg ggtatttggc agtgaagtca
     6421 ttaatgctac tgcggtaggt ctgctggcag tttcgctgat gctggcgctg cacgttgtgc
     6481 cgtggaaaga cattactcgc tataacagcg catggaacac gctggtcaac ctggcaacgc
     6541 tggttgttat ggcgaatggc ctgacccgtt ctggctttat cgactggttc gccaacacga
     6601 tgagtacaca cctggaaggc ttctcaccaa acgcaacggt gattgtactg gttctggtgt
     6661 tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc atgctgccgg
     6721 ttattctggc cgtcggtaaa gggattccgg gcgtaccaat ggaacaactg tgtatcctgc
     6781 tggtactgtc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt cctggggtga
     6841 ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc gcaatcttcg
     6901 gggtgattta catctctatg ctgctgttgg ttggctggcc gattctcgcc atgtggaact
     6961 aatacgttcg ccaaacgctg aataaatcat cacgcccgcc aggtaagccc accaggcggg
     7021 cttttttatg atttaataga taatattcag tgtatgtatg agttccacac ccattatgaa
     7081 agcattctgg cgtaacgccg cgttgctcgc ggtttctctg cttcccttct cttctgccaa
     7141 cgccgtggcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc tggccctctc
     7201 ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac gagatgaatg
     7261 tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg gtctgtggcc
     7321 aggattgcct aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga tgcgcttcgg
     7381 ttgcgctact cgcccaatcc cgaatctacc agaagcgcgc gccagccgaa tgtgttcatc
     7441 gccggaaacc gggttatcac tggaaacggc cgctaaacta agtgaagtca tgccaggagc
     7501 tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct gctttggttt
     7561 tgatccagac gcatacttcg gcacgatggt acgcctgaat caagaaatta aagagagcga
     7621 agccggaaaa ttccttgcgg ataactacgg taaaacagtg agccgccgtg actttgatgc
     7681 cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa cgtgccaggg
     7741 taaccctgcg tatttgactg aaattcagat ctcgatcaaa gctgacgcca tcaacgctcc
     7801 gctttctgcc aactcattct tgccacaacc acacccaggc aactgtggca aaacctttgt
     7861 gattgataaa gcaggttatt aatcctctga tttacaaaac acgctaattc gtcttccata
     7921 aaccatgacg attaagattt atacataatg cgctacattg aggaattccc tgaatgtgac
     7981 gcaaatcact tcaaagagtg agcaacagtc agtatcatca tgtgagttaa accctcgccg
     8041 cctgacggtg agggttttct tttgggtatg tttatctgcg acactcgcag taccgacgac
     8101 atggagtaaa aatgtccaga ccaactatca tcattaacga cctggatgcc gaacgcatcg
     8161 atattctgct ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag
     8221 agttggatcg cgcccaaatg tgttcgccag aagagatgcc acacgacgtg gtgacaatga
     8281 acagccgggt taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt
     8341 atccggcaaa aatgaccgac agcaatactc agctttccgt tatggctccg gtaggtgccg
     8401 cactgctggg gctgcgcgtt ggcgattcca ttcactggga actgccgggc ggcgttgcaa
     8461 cccaccttga agtgctggaa ctcgaatacc agccagaagc tgctggcgat tacctgcttt
     8521 aatcccatct gcacaagcga taactcgctt tgcccagagg atgcactcgc atcctctttc
     8581 cgctcaaata tcaaccagat ttatcatttt gctcttaagc gcagcattct aatctgaact
     8641 ggttattcat ctgcggaacc tcatatgagc gaagctctcg gtatcactgc attttatata
     8701 ttcatagcag caattattgt tgctgcagtg ctataccttg aacaacgctg gtagcgcctg
     8761 gtttttattt attttccagc tcaatagcaa attcagatca aatcagcctc gcaaattata
     8821 gattcataac caataatgtg ctgttatgta tacatgcaaa aacaaggaga gaatgcttat
     8881 gtacaaaaca atcatcatgc cagtggatgt tttcgaaatg gaattgagcg acaaagcgat
     8941 tcgccacgct gaattcctcg cccaggatga cggagttatt catctacttc acgtactacc
     9001 ggggtcagcc agcctgagcc tgcaccgttt tgccgctgat gtacgtcgtt ttgaagagca
     9061 tctgcaacat gaagcagaag aacgtctgca aacgatggtc agccacttca ccatcgatcc
     9121 ttcccgcatt aagcaacatg tccgttttgg tagtgtgcgg gatgaagtta acgagttagc
     9181 gaaagaactt gatgcagatg tagtagtgat tggttcgcgt aacccatcaa tttcaactca
     9241 tctgttgggt tctaacgcct cgagcgtgat ccgccacgcc aatttgccgg tgctggttgt
     9301 gcgttaattg ccagaataag tatcccgccc tgcccggagc catccctgca gggcttttta
     9361 atgcaataaa aaagccgcca ggtttgaccc ggcggctaag caattgcagg tgaatcttac
     9421 ttcttcttat gcagttttgg tgcgaatcag gtagtcaaaa gcactcagag aggctttggc
     9481 accttcgcca gtggcgatga tgatctgctt gtacggaacc gttgtacagt cacccgctgc
     9541 gaacacgcct ttcacgttgg tttcgcattt cgcatcaatg ataatctcgc ccatgcggtt
     9601 acgttcgact gcgccttcca gccagttggt gttcggcagc agaccaatct gcacgaaaat
     9661 accggccagt tcgatgttgt gaatatcgcc gctgacacga tctcgatatt ccagacctac
     9721 gactttgcta ccgtcgcctt tcacttccgt ggtttgcgca ttcagaataa tgtcgacgtt
     9781 tttcaggctg cgcagtttgt cctgcagaac ctggtcggct ttcatttctg gcgcaaattc
     9841 cagcagcgtt acgtgctcaa cgatacccgc caggtcaatt gccgcttcca cgccggagtt
     9901 accgccgccg ataaccgcta cgcgtttacc tttaaacagc gggccgtcgc agtgcgggca
     9961 gtaggtcacg cctttggtgc gatactgatc ttcgcccgga acgttcatgt tgcgccattt
    10021 tgcaccggtc gccacgataa tgctgcgtgc tttcagtacc gcgccagaag ctgtttcaat
    10081 ctgatgcagg ccaccttcaa ccgctgccgg gatcagtttg ctggcgctct ggctgtcgat
    10141 cacatcaacg tcgtattcat caacgtgaac tttcagcgct cctgccagtt tctggccttc
    10201 ggtcttcggt acagaaatgt agttttcgat atcaacggta tcgaggatct gaccaccaaa
    10261 acgttcgccc atcagaccgg tacggatgcc tttacgtgcg gagtaaattg ctgccgctgc
    10321 acccgccggg ccggaaccaa cgattaatac gtcataagca tcacgcttat tcagctcttc
    10381 tgccgcacgt ttttccgcac cagtatcaat tttggcaacg atttcagtca acgtcatgcg
    10441 gccctgacca aactctttcc cgtttacgaa cactgccgga acgcccatca cgttgcgatc
    10501 ggtgatttcg ttctggaagg tgccgccgtc aattgcagtg tgcttgatgc gcgggttcag
    10561 tacgctcatc aggttcagcg cctgcaccac gtccgggcag ttgtggcaag agagcgagta
    10621 ataggtttcg aattcaaaat caccgtcaat atggcgaatc tgctccagca gagactgcgc
    10681 ttctttcgac ggatgaccac cggtccacaa caacgccagt accagcgagg tgaactcgtg
    10741 gcccagcggg gagcctgcaa aacgtggtcc ctggttggaa cctgggttgg tgatcaggaa
    10801 agacggctta cgcaccggca agctgttatc ttctttaaag gtgactttgt ctgacagttc
    10861 tgcgatttca gccaacagtt ccttgatttc tgccgattta gcgctgtcat ccagcgtggc
    10921 aattaactca acaggcttgg tcaatttctc aaggtaagcc ttgagttgag ttttcatatt
    10981 tgtgtcgagc atgtttatct cctgggcttt aaacatcatc atgcaagcgg cctctgcggc
    11041 tacctggatg cagcttgcat catggtgcgg ggaagaaaat cgggtgccgc agcacccgaa
    11101 gaattaggtg aatttccgaa gtatctcgcg ccggagttag tagcgcgaaa gacaaaggaa
    11161 atttagattt taccaaccag gtccagagac ggagccagag ttgcttcacc ttctttccat
    11221 ttagccgggc aaacttcacc tgggtgagaa gctacgtact gtgctgcttt gattttacgc
    11281 agcaggtcag acgcgtcacg accaatgcct tcagcggtaa cttcgattgc ctggatgata
    11341 ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac cttcatcttc acgcatgttg
    11401 tcgaagttac gggtcagggc gccagtcggg tcgccgatca tcgcatattt gattttagcg
    11461 atggtttcag agctgctgtg ccatgctttg tgggtgaagt gagtatcggt agatactgcg
    11521 tatacgtcta cgcccagttt ctgcaattct tcgtaatggt cagcaacgtc acccagttcg
    11581 gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga agacgctcca gcggccttcg
    11641 gtatcttttt cggtgatttc gatgaattcg ccgtttttga atgcctggtt tttaaaaggt
    11701 ttaattttgg tgttaatcaa ggacatctat acttcctccg tgttttcgat gagatgtaag
    11761 gtaaccaatt tttgccgatt cggctaatgc gtttccatta tcaaatcgat aagttttacc
    11821 ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc cagcggcgat aaaaagtaaa
    11881 atggccccct ttccaggtga ccgtgtaatc tgaactaccc tttggtatct tcagcgcctg
    11941 cctgcaggct gaggtgttgc ccttacaaat gcaacaacga catggattac aacaccctca
    12001 taaacaaagg gcaatcacct gatctaagct cttacctatg acagtgatag gttatgcctt
    12061 ttactcgact tttgcactga ctgaaaagga caaattaatg ttaaaaaaga tacttttact
    12121 ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct gccccagtaa aagcgattga
    12181 aaaacagggt attacaatca tcaaaacgtt cgatgcccct ggaggaatga aaggttatct
    12241 cggtaagtat caggatatgg gcgtcaccat ctacctgact ccagatggta agcacgctat
    12301 ctctggttac atgtacaacg aaaaaggtga aaacctgagt aacacgctta tcgaaaaaga
    12361 aatttacgca cccgccggac gtgaaatgtg gcaacggatg gaacaatccc actggctcct
    12421 cgacggtaaa aaagacgcgc cggtcattgt ctacgtcttc gccgatccgt tctgcccata
    12481 ttgtaaacag ttctggcagc aggctcgtcc gtgggtagat tctggcaaag tgcaattgag
    12541 aacattgttg gttggggtta tcaagccaga aagcccggcg acagcagcgg caattcttgc
    12601 ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc tctggtggca agcttaagct
    12661 aagcgtgcct gcaaacgtaa gcacagagca aatgaaagtg ttaagcgaca acgagaaact
    12721 catggacgat ctgggggcaa atgtcacacc agctatctat tacatgagta aggaaaatac
    12781 cctgcaacaa gccgtcggat tacccgatca gaaaacactt aatatcatta tggggaataa
    12841 atagaatata attcatcgat ggttgaaatc tctctctgct tttcaaccat cctaaaaacg
    12901 catgaattgc tcgtaaccca cctgacagct atgctatttt agaaaatctt aatccaaagt
    12961 taagcagaaa aacataatac atataaatac aacaacatta ttgatcaagt gaatgattta
    13021 tggaagagac aatacaacgg agtatgttat ggctaatctt tatgatctaa aaaaatttga
    13081 cctcaattta ctggtcatat ttgaatgtat ttatcaacat ctcagcatta gtaaagcggc
    13141 agaatcatta tatataacgc cctctgctgt tagtcagtcg ttacaacgtt tacgtgcgca
    13201 atttaatgac ccgttattca ttcgctcagg gaaaggtatc gcgccgacta caacagggtt
    13261 aaacctgcat caccatcttg agaaaaatct cagaggcctt gaacaaacga tcaatatcgt
    13321 taataagtct gaactaaaga aaaattttat tatctacggt cctcagctta tttcttgtac
    13381 taataacaac atgctaatcc gctgtcttag acaagatact tcaatcgata tagaatgcca
    13441 tgacatactg ctttcagctg aaaatgccga agaactgttg gttcaacgca aaactgacct
    13501 ggcaattacg ctgcaaccag taattagtcg ctctgtcatc tgcatgccgt tacacactat
    13561 tcgcaattcc cttatatgta gcaacaaaca tcctcgtatt actgatacca gtaactatga
    13621 acaaattatc gccgaagaat tcaccctgtt gatatctaaa tcagcaggga ttgacgaaat
    13681 tcaaatggac atagatgaac gttttatgaa ccgtaaagtt tcatttcgca gttcttcttt
    13741 gatgactata attaatagta tttctgttac tgacttatta ggcattgtgc ccaccgaact
    13801 gtacgattta catcgtgact ttttgaagtt aaaagaaata aaactggaac aacctcttcc
    13861 tgcggttaaa ctttatatat cctataacaa ggcttcgttg aacaaccttg tcttctcccg
    13921 attcatagat cgtttaaatg atagcttctg attcattacc actgacaaat aatatattac
    13981 gcatcaatta attcattgtg tgaatataaa ataatgtgct ctatcttgct tcgtttatcc
    14041 tataattagc ggattcatct cacgaatcac cccgtaacgt tcagagagcg tttttagatg
    14101 tctatttata aaatcccgct tccactgaat attctggaag ctgcaaaaga acgtattacc
    14161 tggacactta ataccctacc ccgtatctgt gtttcttttt cgggtggtaa agattccgga
    14221 ttaatgctgc atctgaccgc cgaaattgcc cgacaaatgg gcaaaaaaat ctgcgttttg
    14281 tttatcgact gggaagcgca gttctcatgc actattaact atgttcagtc attacgtgag
    14341 ttttacgccg atgtcatcga agagttttac tgggttgcgc tcccgcttac gacgcaaaat
    14401 tccctttctc aataccaacc cgaatggcag tgctgggaac ctgatgtcga atgggtgcgt
    14461 cagcccccgc aagatgcgat aaccgatcct gactttttct ccttttacca gcctggcatg
    14521 accttcgaac aatttgtgcg tgagtttgct gaatggtttt cacaaaaacg tccggcggcg
    14581 atgatgatcg gcatccgtgc agatgaatcc tacaaccgtt ttgtcgccat cgccagttta
    14641 aataaacaac gttttgccga tgataagccc tggaccaccg ccgcaccagg cggtcatagc
    14701 tggtacattt accccatcta cgactggaaa gtggctgata tctggacctg gtatgctaac
    14761 catcaacagc tctgtaaccc actgtataat ataatgtatc aggcaggcgt ccctctgcgt
    14821 catatgcgaa tttgcgaacc ttttggcccg gagcaacggc aaggattatg gctctatcac
    14881 gttatcgaac cggatcgctg ggctgctatg tgcgcacgag tcagcggggt aaaaagtggc
    14941 ggcatttacg ccggacatga taatcatttt tatggtcatc ggaaaatcct caagccagaa
    15001 catttagact ggcaagaata tgcattattg ctgctcaata gcatgccgga aaaaacggct
    15061 gagcattacc gcaataaaat cgctatttat ttgcactggt atcagaaaaa aggcatcgaa
    15121 gttccacaaa cccagcaagg ggacattggc gcgaaagata tcccctcctg gcggcgaata
    15181 tgcaaagttt tactcaataa cgattactgg tgccgggcat tatcatttag tcctacgaaa
    15241 gcgaagaact atcagcgtta taacgaacgg ataaaaggaa aacgtcagga atgggggata
    15301 ctatgcaaca acgattaacg caggatttaa cccaatttct cgccagcttg ccggaagatg
    15361 atcgcatcaa agcgattaat gaaatccgca tggcgatcca tcaggttagc ccctttcgtg
    15421 aagagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct aatgattaca
    15481 acccaaacaa cgtggcacca cccgaaaaaa aactgctgaa aaaatccatt gaaatagatg
    15541 gttttaccca acccattgtg gtcacgcata cggataaaaa tgccctggaa atcgtggatg
    15601 gttttcaccg ccatgaaatt ggtaaaggct catcatcgtt aaaattacgc ttaaaaggtt
    15661 atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg actattcgtc
    15721 ataatcgcgc tcgtggacgt caccaaatca ctgccatgtc agaaatcgtc cgagaactca
    15781 gccagttggg atgggacgat aataaaattg gcaaagagct gggaatggac agcgacgaag
    15841 tgttgcgcct gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt caattttccc
    15901 gcgcctggac ggttaaataa ctacagctgg cgcaggcgtt ctgctgctgc cagcaacgtc
    15961 gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc ggcgcaaaac
    16021 accgacagcg gaatcgccgc tacgccgtgc tcccgcgtca gccactggca aaactcaaca
    16081 tcatccagcg tagatacggc gctgtaatcc accagcaaaa agtaagtacc ttcgcacgct
    16141 aagatctcca gccgactctc acttaaggca ttcaccagaa tatcgcgctt ctggcgataa
    16201 aagtccggta acgcaagata gtgctcaggt tctgcatgta gcatatcggc aagcgccagt
    16261 tgtgccgggg tattcaccga aaaggtcaga tactgatgca ctttgcggat ctcggcgctg
    16321 atgggcgcag gcgcaacgca ataccccact ttccagccgg tcatatgata ggtcttgcca
    16381 aacgatgaca ccgccaccgc tcgctcacgc aattgcggat gcgccagcac gctggcatgg
    16441 ccctgttgtg aaaaattgat gtgctcgtag acttcatcgc taatgacaaa aatctcgtgc
    16501 ccggcgatcg cctgccacag agcggcgaaa tcagtctgct gccagacagt tgcactgggg
    16561 ttatgcgggg tgttgaggat caccagtcgg gtgcgctcgc ttaacaatgc ggcaaattcc
    16621 tgccagtcaa cgcgaaaatg cggcggttgc agtgcaatac gtttcactat tccccccgaa
    16681 agtgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac ttcgtcgcca
    16741 ttgcgcacca atgcggttat cgccgcgtat aacgcctccg tcgcccctgc cgtcacggtg
    16801 atattgctgt cgacatccgg ttgatagcca tacagacgtt ccgttttctg agcaatcgcc
    16861 tcgcgcaagg cctgcacgcc ggtcatgggt gcgtattggt ttgccccctg ggcaacgtgg
    16921 tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc ttgcgaaagg
    16981 ttaatcgcct ggtgttgctg cgccagcgcg ctcatccggg tgaaaatagt ggtgccaagt
    17041 tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt acctgacgaa
    17101 gttgcgtgtt gaaggcacta taacacgatg ctagtatttg gcaatcaaga cgtttagatg
    17161 tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc gtggtcgtcg
    17221 gcccggctaa ctacttttca catcccggaa gtttcaatca cctgcacgat tttttcactg
    17281 atgaacaact ttctcgcgcg gtgtggatct acggcgaacg cgccattgct gcggcacaaa
    17341 ccaaactgcc gcctgcgttt gaactgccag gggtaaagca tattttgttt cgtggtcatt
    17401 gcagcgaaag cgatgtgcaa caactggcgg cggagtccgg tgacgatcgc agcgtggtga
    17461 ttggcgtcgg cggcggcgca ctactcgaca ccgcgaaagc cctcgcccgc cgtctcggtc
    17521 tgccgtttgt tgccgttccg acgatcgccg ctacctgcgc cgcctggaca ccgctctccg
    17581 tctggtataa cgatgccgga caagcgctgc attatgagat tttcgacgac gccaatttta
    17641 tggtgctggt ggaaccggag attatcctca atgcgccaca agaatatcta ctggcgggga
    17701 tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggccccgcaa ccagaaacgt
    17761 tgccgctaac cgtgcggcta gggatcaata atgcgcaggc cattcgcgac gtcttgttaa
    17821 acagtagcga acaggcgctt gccgatcagc aaaatcagca attaacgcaa tcattttgcg
    17881 atgtggtgga tgccattatt gctggtggtg ggatggtcgg tggtctgggc gatcgtttta
    17941 cgcgtgtagc ggcggcccat gccgtgcata acggtctgac cgttttgcca caaaccgaga
    18001 agtttctcca cggcaccaaa gtcgcctacg gaattctggt gcaaagcgcc ttgctgggtc
    18061 aggatgatgt gctggcacaa ttaaccgggg cgtatcagcg ttttcatctg ccgactacgc
    18121 tggcggagct ggaagtggat atcaataatc aggtggagat cgacaaaatg attgcccaca
    18181 ccctacgtcc ggtggaatcc attcattacc tgccagtcac gctgacacca gataccttgc
    18241 gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tacaaattta gcagcagcgc
    18301 gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc ctcgtaggtc
    18361 atcggcgttt gatcgggatg gttaacccgc atatgttcga catagttgcc gtaatcaggc
    18421 ataccaatca tcagtttcgc cgcctgacct aaatattttc cggcttttgc cagtgaatca
    18481 aacatctaaa cctccggtaa tacaaatgcc ctcctgaatt aagagggctt aattgctatc
    18541 aacacgctcg ggatgagagt aaggggaacg ctcagcacat tccccctcac tctgacttta
    18601 gtgcgcgcct ttcgcctgcg ccacgatctc ctcgacattt tccggcatcg gctcatatgg
    18661 cgtttctttc gccgttggct tcggctcttt caatgccacc agtgccgtct taatcgagaa
    18721 cagcgccaga accaccacaa ccaccataaa gaagatggtt aatccggcat ccagacggtt
    18781 gttgaacacc agttgcgcca gttgtgactc agtatactgc gacggaatat tgccgctgtc
    18841 gatcattgcc tggaacttgt tagcaatcgc caggaagccg actttcgcat ccgggctaaa
    18901 cgctttctgc cagcctgcgg tcagggtaca aatcagcagc caggccgttg gtaccagcgc
    18961 cacccaggcg taacgttgac gtttcatttt gaacagcacc acagcacaga gcatcagcgc
    19021 catccctgcc agcatctggt tggcaatacc aaacagcggc cacaaggtgt taataccgcc
    19081 cagcggatcg accacgccct gatgcaggaa gtagccccac gccagcacgc acagcgccgt
    19141 tgccagcagg ttagcaggca gtgaatcggt ccgtttcagg cccggagaca ccacgcccag
    19201 cagatcctgc aacataaagc gcgcagcacg cgtacccgca tccaccgccg taagaataaa
    19261 cagtgcttca aacagaatgg cgaagtgata ccagaacgcc acatccatca tgccgcccag
    19321 cgcaccgtgc agaatgtagg ccatccccac cgccagggtc ggcgctccgc ccgcacggga
    19381 aatgatcgac tgttcaccca cttcgctggc aatctggttt agcgtatccg gggtaatcgc
    19441 aaagccccag ctactcacca cctgcgcggc agaagcaacc acatccgccg tccctgccgg
    19501 agccagcacc gccatcgggc tgttcatggc gaagtacaca cccggatcga tgatacaggc
    19561 ggaaaccagc gccataatcg ccacgaagga ttccatcaac atcccaccgt agccgataaa
    19621 gcacgcctgc ccttcgttcg ccagcatctt cggtgtagtg ccggaagaga tcagcgcatg
    19681 gaagccagac accgcgccac aggcgatggt gataaacagg aacgggaaca ggttaccggt
    19741 ccataccggg ccagtgccat cgacaaattt agttagcgca ggcatggtca gcgtcgggcg
    19801 cataatcaaa atgcccaccg ccagaccgac gatagtcccg attttcagga aggttgagag
    19861 gtagtcacgc ggagccagca gcaaccacac cggcagcacc gccgccacaa aaccgtaacc
    19921 caccagcatc caggtcagct gcacgccggt aaagtcaaag tacggtgccc acgtcgggct
    19981 ttctgccacc cagccgccag agataatggc gaaaataagg aacaccagac caatcaccga
    20041 cacttcgcca atacgccccg gacgcaaata gcgcaggtag atccccataa acagcgccag
    20101 cggaatggtg aacgcaacgg tatacgttcc ccacgggcta tgagtcaggg ctttaaccac
    20161 gatcatcgcc agcacggcaa ggataatgac catgatcata aagcaggcca ccagcgctat
    20221 caccccggcg gttggcccca tctcttcttt gaccagctca cccagcgagc gaccgtcacg
    20281 gcgcgtggaa acaaacagca ccatgaaatc ctgcaccgca ccggcgagaa ccacccctgc
    20341 gagcagccag atcatccctg gcaggtaacc catttgcgcc gccagtaccg gcccaaccaa
    20401 cggacctgct ccggcaatgg ccgcaaaatg gtgaccgaac agcacttttt tgtccgtcgg
    20461 cacatagtcc agcccgtcgt tatggcgcac cgctggcgtc atacgcgtcg ggtcaaccgc
    20521 cagcacattt ttggcgatat acagcccata aaaacggtaa gcgatcagat agatacagac
    20581 cgacgccacc acaatccaca gcgcgttgat ctgttcccca cgatttaaag caatgtatcc
    20641 cagagcaaat gctcccatta cagagagcac tgtccagacg aggtatttcc ctgatttgtt
    20701 catagttgtt atccgtgtgc gtgtccatag agatgttaca ttttgtttct ataacatctt
    20761 tgtacaattt aacaacacag caaccatgta aatcggagtt gaaaccaata tttaacttaa
    20821 caatgttaac tcgatcactc cgttaaccga attttcctgc aatctcattt tattctgtcg
    20881 ggttcatccc aaaaccgctg tgcccagccg acaggtgcag caacgccgcc cctgttcatc
    20941 gaaaacgacg atttcccagc tctgattttg tcgtccaaga tgcagcggct ggcagacacc
    21001 gcgcaccttt ccctcagaca ccgggcgatg atgtgtggcg ttaagttccg tgcccaccac
    21061 acactgtccg tcgcgggtca tcataaatcc ggccatcgat cccagcgttt ccgccagcgc
    21121 cgccgacgcg ccgccatgca gcaggccaaa cggctgatga gtacgggtat caaccggcat
    21181 ttcggcttcg agtacatcat cgcccagacg ggtatacaca attcccagat gtgccaccat
    21241 tgtgttatcg ctggtggcgt tcagctcgtc gagcgttaaa tggcgtttcc agatcatgct
    21301 tatgccccca gcgttgagcc gccatcgacc acaatatcct gtagggtaat atggctggcg
    21361 aggtcagagg cgaggaacaa aatcgtattg gcgatctcct gcggacgggc gattttcccc
    21421 agcggaatgc cgagtttaaa ctgctcgcca aagccgcgaa tacgctgctc ttcggcgtca
    21481 tcgctcaccc acagcgtgcg ttgcatatcg gtatcggtgg aacccggtga aaccacatta
    21541 cagcgcaccc cgctacccgc cagttccagc ccgacgctca acgccaggct tttcagcgcc
    21601 gctttcgacg cgccataagc actcatacca atgcgcggcg tgtgtgcggc gtcggacgca
    21661 acagtaacaa tcgccccgcc ccgctgacgg cgaaactggt tcatggtctg ctgaaacagg
    21721 ttaaatgcac cgccgacgtt gaccgcaaaa gtctgctgcc agtcctcttt actgagctga
    21781 tcggtcgcgc ccatgcgcaa aattcccgcc gcattgacca gcacgtccag tcgctccgtt
    21841 tcagctaaca gtcgctgaca tacctgcgcc acctgcgcag catcggcaac atccattact
    21901 tcggtcgcaa aaggatattg ctcctgagta aacgcttgat caaaacctgt aactttcgcc
    21961 cccgcctcaa caaatgccag cgccgtggca tagccgatac ctttccccgc gccagttacc
    22021 cagacatttt taccgctaaa atccattatt tcacctcgcg ggagagtagc ttccaccagg
    22081 cgtcgatggt cgggtttttc gccagcatga caaagtcgat atcgccatgc actttgcgcc
    22141 agcgcgccgc cagcgccatc atgcgcaccg aatccagacc gtagtcgatc aggttgtcgt
    22201 catcgaaggg ttcatcggac tcgtccagca acggcaggat cacctcacgc agctccgctt
    22261 tgctggcggg gataggtgct ggcagcaatt cttcggtcat caccacccga ccagaacgtc
    22321 cggccacata tttcagcgac atcaaatgct cgtcacggct gaaatcggcc agcgcatccg
    22381 ccaccataaa cggtttaata tcgcgcataa atgcgtccgt tgcggtggtc atacagccaa
    22441 tgtgggcata taccccggta ataatcagct ggttacgtcc actctctttg agcatttgct
    22501 ccagcgggga acgatgaaac gcgctgtagc gccacttcac cagcaccgtg tcgtcggcat
    22561 ctggcgtcag gcgatccacc accttttgct gttccggcga acgggtcagg ccaggccccc
    22621 acatatcatt caacagcgcc cgatcttcat cgctctgctc tttcggctgg gccgtgtaat
    22681 aaaccgggat attgtgctgt ttgcagtagt cgcgtagtgc cgcaatattc gcgatcacct
    22741 gctccatcat cgggcagttc tcaccccaga agctgacaaa atagtcctgc atatcatgga
    22801 ttaacaacgc ggcacgttgc ggttcaaagg cccagtcgac tttattctgc ggaatatcat
    22861 gagactccgg cagtgcgtaa gcctgtaatt ttggaatagc catcgtgttc tctccttcag
    22921 gctgatgtgc gtgacgccag ccactgacgt aattgttttt tatcgacttt cccgaccgcc
    22981 gtcagcggaa gtgaatctac acactccacg cgatccggta atttaaattc ggcaataccc
    23041 tgttcacgca ggaaacgacg cacctgcacc gcgcgcagcg gctcttttac caccagataa
    23101 gcacagcttt tttcgcccat cagctcatct tccatgctca ccagtgcggc gtagatcacc
    23161 gccggatggc gcagcagcag gttttcgatc tcttcggcag cgatcttctc gccgccacgg
    23221 ttgatctgat ctttctcgcg cccctgcaca gtgatgtaac cctctggatc aatagagatc
    23281 agatcgccgg aacagtaaaa accgttagca tcaaaggcgc tggcattgtg ctgcgggctt
    23341 ttgtaatagc cacggaaggt gtacggcccg cgcgtcatca ggcgtccaac ttccccttgc
    23401 ggcagtggat ttccttcggc atcggcaacc catacttcat cgtccgggca cattgggtaa
    23461 ccctgggtat ggataatttt ctccgcacta tcatcaagac gggtgtagtt caccagccct
    23521 tccgccatgc caaacacctg ctgcaactga cagccaatct cagcgggaat acgcgccgca
    23581 agggtggcag aaagacgggc gccgccgacc tgtaacagtt tcagcgaggc aagctgcgcc
    23641 cggctttcgc cttcagccag tgcctgcaac cacaggctga ctgctggcgg caccagcgcg
    23701 gtgacgttca gctgatgttt ttcaatcaat gggaagcaaa gcgtggcgct ggggtcggca
    23761 gccagaacga cagtgccgcc agcgagaaat acccccaacg atcccggcga actcatggcg
    23821 tagttatgag ccgccgggat cgcgcacagg tagcgcgtct gttgtgtgaa ctgacaaatc
    23881 tcgacgctac gacgcacgct gtagtagtag tcattgtgag tgcgtgggat cagtttcggt
    23941 gtgccggtgg tgccgccgga aagctgaaaa taggccactt catcagcagg agatggcgtg
    24001 gcagtaaaat cctcagccgg atggttaatc gcatcctgca agttatgctc gccgctgtcg
    24061 ttgagcagct gcaccacacg aatggaagaa tgctctgcaa caaatgtgtt gaggaaatca
    24121 tccccgctaa atagcgcatg ttggcgatcg gcaatcagta acgccggttc aatctggctg
    24181 gcataggcgt tcagttcact acgctgatgg ctgaacagcg ccagcaccgg cgcaacgccc
    24241 agtttcagca gcgcgaaaaa ggtaatgtaa agttcagcaa cgttacccag ttgtaccagc
    24301 gcggtttcac caggtttaat gccctgacgg cgcaaactac aggcgaggtt atccgccgcc
    24361 tgattcagct cccggtaact caactgccgc tcgccgtcga taaccgcgat gctgtcactc
    24421 gtagcgtggc gagtcagaat gtcggtcagt ggcaaatcct gccagtagcc tttttcccga
    24481 tagcgacggg caaactcttc cggccagcgg gtgaatggaa tgctcatcct cgctccttaa
    24541 tgcaatccaa aaacgttcaa catggtagaa agtttgacgc ctgtttcgcg ccactcaccc
    24601 aacggtgagg acgcaggcac aatccccgct ccggcaaaca gacgcacctg attttcccgc
    24661 agcttcgcgc agcggatggt caccacccat tcaccgttac cttcgctgtc acaccaaccc
    24721 acaatgccgc caaacagttc gcggtcgaac ggctccagtt cagcaataac ctgagtcgcg
    24781 gcctgatgcg ggaaaccgct cagcgcgggg gtcggatgca gcagacaagc caaagtcagt
    24841 gcgttttctt gcgaattcgc tttaccttca aagggcgttg cgagatgcca cagcgtcggc
    24901 gtggtaatca attgtggaga ggagggaacg tgtaactcac tactgcgttt gcgcagtacc
    24961 tctttcatcg cctgagtcac cagttcatgt tcatggcgat ctttttctga cgccagcaga
    25021 cgattacccg cttcgcgatc gagcacttca tccggctgac gacgcgcgga accggctaac
    25081 ggaatggagc taaaacgctc gccgtcttta cgtagcagca gttccgggct ggcccccagc
    25141 agaacgccac catcagccaa cggaacatgg aagttgtaac taaccgggtt ttgcgcaatc
    25201 aaccgttcca gcaatacgcc actatcaatg gcggcgtcag tagtgatatc aatcaaccgt
    25261 gacaacacca ctttgtcgac ctgcggcgtg gcggtaagtg cggcagcacg ggcaaccatc
    25321 tgttcaaacg tggtttgttc cggaatggcc tgacgttcca ccacgttcag cgactggctg
    25381 cgggtgaaac ggcgtgcgga agcttgtttt tcctgacggg agaacgactg ccaggattcg
    25441 ggaatataca gcgacgaagg ctgacgtgga tcgaagggaa tcgccccgac catcaccgga
    25501 tttttgatgc cctgcgcttt ggcatcggca aacagcgcgg cgagtttttg ctggaaggga
    25561 ctgtcgggcg aatccccgtt cacagccggt tcatcgaagc gggcgaaaca tcctgacgtc
    25621 gtaaaactgc ggtacggcga cataaagaaa aagcgattgg gcgcaagtgt tgccatggtc
    25681 tgctgtactt cctcagccag tgacgtatcc atatcatcct ccacaaaatg ataaaggctt
    25741 taataatgat tatcatttat attttcggtc gctaacctaa aagcaaacgc gcactatgtc
    25801 aactcttgag gtaacgcaat ctgcaaatcg gggttgcatc tggttgtggt tacaatgaaa
    25861 atgagaagca ttaacttcat taataacagg aagctgactt gtgagactcg ccccgctcta
    25921 ccgcaacgcc cttctattaa caggactttt gctttcagga atagcagcag ttcaggccgc
    25981 cgactggccg cgtcagatta ctgacagccg tggcactcat accctggaaa gccagccgca
    26041 gcgtattgtt tccaccagcg tcaccctgac cggctcactg ctggcgattg atgctccggt
    26101 gatcgccagc ggcgcgacca cgccaaataa ccgcgtagcg gatgaccagg gctttttacg
    26161 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    26221 tgcagaagcc gttgccacgc aaatgccgga tctgatttta attagcgcaa ccggcggcga
    26281 ttcggcgctg gcgctgtacg atcagctttc taccatcgcc ccaacattaa tcatcaatta
    26341 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    26401 acaagcggca gagcggattg cacagtttga taagcaactg gcggcggcga aagagcaaat
    26461 caaattaccg ccgcagccgg tcactgccat cgtctatacc gccgctgcac acagtgccaa
    26521 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    26581 gaagttgccc gcaggcttaa acgccagcca aagccagggc aaacgccatg acatcattca
    26641 gcttggtgga gagaatctgg ctgcagggtt aaatggcgag tcactattcc tgttcgccgg
    26701 tgatcagaaa gacgccgatg ctatttatgc taatccgctg ctcgcgcacc tgcctgcagt
    26761 acaaaacaag caggtttatg cgctgggaac cgagacattc cgcctggatt actacagcgc
    26821 catgcaagtg ctggaacgac ttaaggcgct gttttaagca ttaattgtcg gacgctgtca
    26881 cctgcggcgg cgtctggcga aaacgccgca actccaccag caccagcaac aacaacacgc
    26941 cgataatcaa caaaccaaaa ccgctcgcac ttgcggaagc aaccggtgtc atcatcgctc
    27001 ccaaaccgcc cagcagcgcc gcgcctatgg catcgcccgt cacgttttgc gccgtccaca
    27061 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    27121 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    27181 acatcggcat caggccaaac agaccaatgg cgaggaacgc ccccaatgtg gagagcaaca
    27241 tcaacagacc tggccgtacg ctatgcgcca gttttccact ggttaacgcg ccaatagccg
    27301 caccgagcgg gattgccgca tagagaaagc caatttgcgc cgccgacatc tgccagttgt
    27361 cagccagcgc cggatacagg acccgcaccg cgctcgccat cgtcaataaa ccccccagca
    27421 acgcaatccc tcccaccagc gggctggcga gtagaaaacg aaatcctgcc agtaatgatt
    27481 tcaacggatg ctcacgcggc tgcggcggtg gtggcaacgc cggaaggctt aacaacggta
    27541 gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg cctccggtcg
    27601 ccagcagtaa accgccaatc atgggcgaaa tcaccgatcc cagacgcacg gtcaacatgg
    27661 tgatcgcccc ggcctgcatt aggttttcac gccctaccag tgcaggtgtc gccgccagta
    27721 gcgccgtaac gccaagtgat gcgaaaaaac catcccataa accaagtaaa tagattgcca
    27781 gcaaagacgg ctccggcagc agcgcattca ggcacagtcc aatgaagcca atgccacagg
    27841 tgccgcgcgc cagcaaaatc acttttttac gctcatagcg atccgccagc acaccgccga
    27901 ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc acctgccagg
    27961 tggaatgcgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa cccagagaaa
    28021 caattgagat aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc
    28081 tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa atattttaaa
    28141 tacctattct taggctgcac atgctaacat atccaaataa gatcgataac gataattaat
    28201 ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc cattgccgtg
    28261 cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat tggagcaaaa
    28321 tcactccccg cttccgtagt gctggaggcg ctctccggca cctgccagag cgccgactgc
    28381 accattgtgc tcgacgcccg tctgccgcgc acccttgccg gtttactggc aggcggtgcg
    28441 cttggccttg ccggggcgtt aatgcaaacc ctcacccgaa acccacttgc cgatcctggt
    28501 ttgcttggcg tgaacgccgg tgccagtttt gccattgtgc tgggcgcggc gctgtttggt
    28561 tactcttccg cgcaggagca actggcgatg gccttcgccg gggcgctggt ggcctcgttg
    28621 attgttgcct ttaccggcag ccagggcggc gggcagttaa gtccggtgcg tttaaccctg
    28681 gcgggcgtgg cgctggcggc ggtgctggaa gggctgacca gcggcatcgc cctgcttaat
    28741 cccgacgtct acgatcaact gcgtttctgg caagccggtt cgctggatat tcgcaatcta
    28801 cataccttaa aagtggtgct gatcccggtg cttaccgctg gagcaactgc gctattactg
    28861 agccgcgcgc tgaacagttt aagccttggc agtgacaccg cgacggcgct gggcagtcgc
    28921 gtagcgcgca cacagttgat tggtctgctg gcaattaccg tgctttgtgg tagtgcgacg
    28981 gcggtagttg gcccgattgc ctttattggc ctgatgatgc cgcacatggc gcgctggctg
    29041 gtaggtgccg atcatcgctg gtcgctgccc gtcacgctac tcactacccc tgccctgctg
    29101 ctgtttgccg atatcattgg gcgggtgatt gttcccggcg aactgcgcgt ttctgtagtc
    29161 agtgccttta ttggtgcacc agtgctgatc ttcctcgtac gacgtaaaac gcgaggtggc
    29221 gcatgattta cgtctctcgc cgattaatta tcacctgtct gctgctgcta atagcttgtg
    29281 tgatggcagg cgtgtgggga ttacgtagcg gtgccgtcac gctggaaact tcgcaggtat
    29341 tcgccgcgtt gatgggcgac acgccgcgca gtatgacgat ggtggtcacc gaatggcgtt
    29401 taccacgcgt gctgatggcg ctgttgattg gcgcggcgct gggcgtcagc ggcgcgattt
    29461 ttcagtcgct gatgcgtaac ccgcttggca gccctgacgt aatgggcttt aacaccgggg
    29521 cgtggagcgg cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg gctatcgcgc
    29581 tggcagcaat ggtgggcggc attgtcactt cgctgctggt ctggctgctc gcctggcgta
    29641 acggtatcga cacctttcgg ttgattatta tcggtattgg tgttcgcgcc atgctggtgg
    29701 cctttaatac ctggctgttg ctgaaagcgt ctttagaaac ggcgctaaca gcgggtttgt
    29761 ggaatgccgg atcgctcaat ggcctgacgt gggcaaaaac ctcgccttcc gcacccatca
    29821 ttatattaat gttaattgcc gccgccttgc tggtgcggcg aatgcgcttg ctggaaatgg
    29881 gcgatgatac cgcatgtgcg ctgggcgtca gcgtcgaacg ttcgcggctg ttaatgatgc
    29941 tggtcgccgt agtgcttacc gcagctgcca ctgccctggc ggggccaatt tcctttattg
    30001 ctttagtcgc gccgcacatt gcccgccgca tcagtggcac cgctcgctgg gggctaaccc
    30061 aggcggcgct gtgcggggcg ctgttactgc tggtggccga tctctgcgcc caacaactgt
    30121 ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt atttacctta
    30181 tcgtcttgtt aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc gtttgcgcgg
    30241 cgaacagtta accctcggat atggcaaata taccgttgcg gaaaatctga ctgtagaaat
    30301 acctgatggt cacttcacgg caattatcgg gccaaatggc tgcggtaaat ccacgttact
    30361 gcgtacctta agccgcctga tgacgcctgc tcacgggcat gtctggctgg atggcgagca
    30421 cattcaacat tacgccagta aagaggttgc acgccggatt ggtctgttgg cgcaaaatgc
    30481 taccacaccg ggcgatatca ccgtgcagga gctggtggcg cgtgggcgtt acccgcatca
    30541 accgctgttt acccgctggc gcaaagaaga tgaagacgcg gtaacaaaag cgatgcaggc
    30601 cacgggaata actcatctgg cggatcaaag cgtggacacc ctttctggcg gacaacgcca
    30661 gcgagcgtgg atcgcgatgg tgctggccca ggaaacggca attatgctgc tcgatgaacc
    30721 cacgacctgg ctggatatca gtcatcagat tgatttgctg gaactgttaa gcgaactgaa
    30781 ccgcgagaaa ggctataccc tggcggcggt gctgcacgat cttaatcagg cctgtcgtta
    30841 cgccagccat ttgattgcat tgcgggaagg gaaaattgtt gctcagggag caccgaagga
    30901 gattgtcact gctgaactga tcgagcgcat ttatggtctg cgctgcatga ttattgacga
    30961 tccggttgcc ggaacgccgc tcgtggtgcc gctcggacga acggcacctt caaccgcaaa
    31021 gatttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca tcgcatggcg
    31081 caacagcaca ccaccacaag ccaccatccc gccgatcaac gcggaaagga tcacaataat
    31141 cgacttaccc ggaccgtctt tttttactgg caatgacggt cttaactgat atttaaacgg
    31201 cgtaaaattc acatcgttga tatttgcttt tgttaactgc tcaacaagat actggcgatt
    31261 acgtaattcg ccgttcagtt ccgcaacgtc agtaaccgct ttttcaattt ccagtttgcg
    31321 ttcaataccg tcagcaccga gagaaataga gaaatcgggg tcatctttaa cggcctgacc
    31381 attactgtat acgggctttt taattcctgc cgcgttggca atgtcgagtg aataattgag
    31441 gcgctgaatg tttgcatcaa gttgattttt cgttttaatg cgatcctgag ccagtttttc
    31501 tttttcaaac tgggttttga tctccagttt attacggacg ttttctatcg actctttcac
    31561 caccaacgca gagatataat cgatataccc ggccaacact ttctgtgcct cttcactggt
    31621 gggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt ttttcttact
    31681 ggcattgtca tcaaccgctt tcattttttc gctcaatgcg acgatagctc gatgcaaatc
    31741 cagttcgtcg attttcgcct cttttaattg gtccatcaca taaggtgatg agcgcaggta
    31801 ctcttctagt aagctaacag actgaaactt cttgataaac aggttaaatg caccgccacg
    31861 gtcgatatta atatcgagat caagaacgcg gagtttggta aaggttttct ccaggtcctg
    31921 ccactgaatg gcttcggcgg gagtaatgac ggctgagctg gtccattttt gcggcaggat
    31981 gaaagagatc agtaaccctg cgcaggcaaa cgcaaaaacg accgccatga ccgttttttt
    32041 ggcccgccat aaaacttcaa ttagactaag taaatcaatt tcattattac tgggcgacgc
    32101 cagaggatac tcgggaaaat gagcttcact tccctgttta atattcagtg atgacatggg
    32161 gtataacctg aaaatgagtc caataagagt aaatctccac ggcccccact ctaccagttg
    32221 gtgagattta tccgaagctg aagtttgtaa attactccca ctgaatgcaa c
//
LOCUS       DADTFG010000042        32206 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.042, whole genome shotgun sequence.
ACCESSION   DADTFG010000042 DADTFG010000000
VERSION     DADTFG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.042"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(54..1814)
                     /locus_tag="J1138_001801"
     CDS             complement(54..1814)
                     /locus_tag="J1138_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBA8453609.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLCLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1944..2636
                     /locus_tag="J1138_001802"
     CDS             1944..2636
                     /locus_tag="J1138_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBA8453610.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2835..3923
                     /gene="serC"
                     /locus_tag="J1138_001803"
     CDS             2835..3923
                     /gene="serC"
                     /locus_tag="J1138_001803"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBA8453611.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3994..5277
                     /gene="aroA"
                     /locus_tag="J1138_001804"
     CDS             3994..5277
                     /gene="aroA"
                     /locus_tag="J1138_001804"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBA8453612.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            5447..6211
                     /locus_tag="J1138_001805"
     CDS             5447..6211
                     /locus_tag="J1138_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBA8453613.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSDNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6384..7067
                     /gene="cmk"
                     /locus_tag="J1138_001806"
     CDS             6384..7067
                     /gene="cmk"
                     /locus_tag="J1138_001806"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBA8453614.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7178..8851
                     /gene="rpsA"
                     /locus_tag="J1138_001807"
     CDS             7178..8851
                     /gene="rpsA"
                     /locus_tag="J1138_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBA8453615.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9011..9295
                     /gene="ihfB"
                     /locus_tag="J1138_001808"
     CDS             9011..9295
                     /gene="ihfB"
                     /locus_tag="J1138_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBA8453616.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9501..11765
                     /locus_tag="J1138_001809"
     CDS             9501..11765
                     /locus_tag="J1138_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBA8453617.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11802..13550
                     /gene="msbA"
                     /locus_tag="J1138_001810"
     CDS             11802..13550
                     /gene="msbA"
                     /locus_tag="J1138_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBA8453618.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13547..14533
                     /gene="lpxK"
                     /locus_tag="J1138_001811"
     CDS             13547..14533
                     /gene="lpxK"
                     /locus_tag="J1138_001811"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBA8453619.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PIPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVNAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDVQLSGDEPAKLLAQLTSLASGN"
     gene            14570..15802
                     /locus_tag="J1138_001812"
     CDS             14570..15802
                     /locus_tag="J1138_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBA8453620.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAELQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15854..16036
                     /locus_tag="J1138_001813"
     CDS             15854..16036
                     /locus_tag="J1138_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBA8453621.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16033..16779
                     /gene="kdsB"
                     /locus_tag="J1138_001814"
     CDS             16033..16779
                     /gene="kdsB"
                     /locus_tag="J1138_001814"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBA8453622.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNVVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16933..17826
                     /locus_tag="J1138_001815"
     CDS             16933..17826
                     /locus_tag="J1138_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBA8453623.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWGYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17803..18582)
                     /gene="elyC"
                     /locus_tag="J1138_001816"
     CDS             complement(17803..18582)
                     /gene="elyC"
                     /locus_tag="J1138_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBA8453624.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18718..19503
                     /gene="cmoM"
                     /locus_tag="J1138_001817"
     CDS             18718..19503
                     /gene="cmoM"
                     /locus_tag="J1138_001817"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBA8453625.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19500..20822
                     /gene="mukF"
                     /locus_tag="J1138_001818"
     CDS             19500..20822
                     /gene="mukF"
                     /locus_tag="J1138_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBA8453626.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20803..21507
                     /gene="mukE"
                     /locus_tag="J1138_001819"
     CDS             20803..21507
                     /gene="mukE"
                     /locus_tag="J1138_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBA8453627.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21507..25967
                     /gene="mukB"
                     /locus_tag="J1138_001820"
     CDS             21507..25967
                     /gene="mukB"
                     /locus_tag="J1138_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBA8453628.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26228..28075
                     /gene="ldtD"
                     /locus_tag="J1138_001821"
     CDS             26228..28075
                     /gene="ldtD"
                     /locus_tag="J1138_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA8453629.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMTRDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNNREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28256..28804
                     /locus_tag="J1138_001822"
     CDS             28256..28804
                     /locus_tag="J1138_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBA8453630.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28831..29478
                     /gene="gloC"
                     /locus_tag="J1138_001823"
     CDS             28831..29478
                     /gene="gloC"
                     /locus_tag="J1138_001823"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBA8453631.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29528..30718)
                     /locus_tag="J1138_001824"
     CDS             complement(29528..30718)
                     /locus_tag="J1138_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HBA8453632.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLVKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30903..31991)
                     /gene="ompF"
                     /locus_tag="J1138_001825"
     CDS             complement(30903..31991)
                     /gene="ompF"
                     /locus_tag="J1138_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBA8453633.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESSLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7882 a         7820 c         8634 g         7870 t
ORIGIN      
        1 cgtaatataa ctcagacgaa tcattaagcc tacattgcgc aggctatttg attttatttt
       61 gcccagaatg ctgctttggc gcgttgcagc ttttcgtagg ccttcaacaa cgactgatgt
      121 gcagcaaacg cgtgcagatc gctatctacg ggttgcaagc cataaaacgc cgcttcgccg
      181 ctcattgccg cactggcggc ttccacggca tctgcgccgt acatgcgaac aaacgcattc
      241 agatattgca gcggctggcg atcttcttcc tgcgccagta ataacaacgt ttgcaggcag
      301 cgataataat tggcgcgttc cgggctgaat actgatgagt taaactccat cgtccattcg
      361 gtccagacca gagcctgttc cagatcgcca ccagccagcg ccagcatggc ttttaattca
      421 ccgatacgca gagtgtacca gccgttatcc gacccggtcg ccagacccaa cagttcacgc
      481 acgcgggtaa agtcatcaaa accttcttca tccagttgct cgatgaggtt caggtaatct
      541 tccttttccc actcgctacc tggtagcgaa agaatcgttt cacgtaaatg gctgcccata
      601 ctgttattcg ccagacacag atcttcagcc ggataaatat cggacatgcc aggcacgata
      661 atacggcaag cgtagacacc cagatgctcg taatcggcaa tataaacttc tttatcttcc
      721 tttttgaaga tagccatcag cgtggcgaac tcttcttccg tggtgccgga gaagctccag
      781 tcaacaaacg gataatcggc atcctgcttg aacaggtccc aggagattaa accgctggaa
      841 tcgataaagt gcgtttcgag gttggtatgt tcagcgactt cttcatcatc gaaggttggt
      901 ggagtaaaca catccagatc tttcaggcca cgaccttgca gcaactcggt cacggtacgt
      961 tccagtgcaa cgccaaaatc aggatgcgca ccgaatgagg caaagcaggt accgttagca
     1021 ggattgaaca gtaccacgca aatcaccgga tactggccgc caagcgaacc gtcataagcg
     1081 aagatcggga aaccttccgc ttccagcgtt tcgatcgctt caactactgc tggataacgt
     1141 gccagcacgt ctgccgggat ttccggcaag ctgatgcttt cagcaataat gcggtttttc
     1201 acgtaacgtt cgaaaacttc ggacaacccc tgaacgcgtg cttcgttacg ggtattgcca
     1261 gcagacatac cgttagaaac gtacaagtta ccaatgatat tcatcggaat ataaatggtc
     1321 tgattgtcgg actggcgcgt aaacggtaga ccgcaaatac cacgatcttc gttaccggat
     1381 tgcaggtcaa tcagcatact accggtcagt tcattctccg gatcgtaaaa tgcgcgcaga
     1441 cggtcatcga gcagcccttc tggtacatcg tcattttcgg tcagtgggaa ccatttttcg
     1501 ttgggataat gcacgaatgg gccgttggcg atggtttcgc ccagccagaa gtccgcaaaa
     1561 aagtagttgg ttgagagacg ctcgaaatat tcaccgaggg cagaagccag cgccgctttt
     1621 ttggttgcgc ctttaccgtt ggtaaaacac agtgcgcact ctttgtcgcg aatatgtaca
     1681 gaccagacgt taggcaccgg attcagccag gaggcctctt caatctgaaa accgaggtct
     1741 gaaagttttt gctggaagcg tgcgatggaa tcttccagag cggcatcttt gccggggata
     1801 aatgtttgcg tcatgaaaat cactttagtc gtacggaaag cgcacaataa tacgggtttt
     1861 atctcaaagg cgctattacc gccgccatgc cgatgaactg ttggctatgc ttttagcgga
     1921 taaaccacga taagagcata aaaatgaagg cgttcgatct ccaccgtatg gcatttgata
     1981 aagtgccttt tgatttcctt ggcgaagttg cactacgtag tctttatacc tttgtactgg
     2041 tttttttgtt cctcaaaatg accggaagac gcggtgtgcg gcagatgtcg ctgtttgaag
     2101 tgttaatcat tctgacactg ggttcagcgg cgggagatgt ggcgttttat gatgatgtgc
     2161 cgatggtccc ggtacttatc gtctttatta ctctggcgtt gttataccgc ctggtaatgt
     2221 ggttgatggc gcacagtgaa aaactggaag atcttctgga aggcaagccg gttgtcatta
     2281 ttgaagatgg cgagctggcc tggtcgaaac tcaataactc caacatgacg gaatttgagt
     2341 tctttatgga gctacgattg cgcggtgtag agcagttggg gcaggtacgt ctggcgattc
     2401 tcgaaaccaa cgggcaaatc agtgtttatt tctttgaaga tgacaaggtg aaaccggggt
     2461 tacttatttt acccagtgac tgtactcagc gttacaaagt ggtgccggag tcggcggact
     2521 atgcctgcat ccgttgcagt gaaatcattc atatgaacgc gggggaaaaa caattatgtc
     2581 cgcgctgtgc aaatccagaa tggacgaagg caagtcgggc taaacgggtg acctgacagc
     2641 caaaacatcg gttttttgaa aataatccga gagattcttt tgtgtgacgc gtgcctcatt
     2701 tttgccctca acggttttac tcattgcgat gcgtgtcact gaatgataaa accgatagcc
     2761 acaggaataa tgtattgcct gtggtcgcaa ttgattgacc gcgggttaat ggcaacgcaa
     2821 cgtggtgagg ggaaatggct caaatcttca attttagttc tggtccggca atgctaccgg
     2881 cagaggtgct taaacaggct caacaggaac tgcgcgactg gaacggtctt ggtacgtcgg
     2941 tgatggaagt gagtcaccgt ggcaaagagt tcattcaggt tgcagaggaa gccgagaagg
     3001 attttcgcga tcttcttaac gtcccctcca actacaaggt attgttctgc catggcggtg
     3061 gtcgcggtca atttgctgct gtaccgctga atattcttgg tgataaaacc actgcagatt
     3121 atgttgatgc gggttactgg gcagcaagtg ctatcaaaga agcgaaaaaa tactgcacac
     3181 caaatgtgtt tgatgcgaaa gtgaccgttg atggtcttcg cgcagttaag ccaatgagtg
     3241 aatggcaact ctctgataat gctgcttata tgcattactg cccgaacgaa accattgacg
     3301 gtatcgctat cgatgaaaca ccagacttcg gtaaagacgt ggtggtcgcc gctgacttct
     3361 cttcaaccat tctttcccgt ccgattgatg tcagccgtta tggtgtaatt tacgctggcg
     3421 cgcagaaaaa tatcggcccg gctggcctga caatcgtcat cgttcgtgaa gatttgctgg
     3481 gcaaagcgaa tatcgcctgc ccatcgattc tggattactc catcctcaac gataacgatt
     3541 ccatgttcaa cacgccgccg acatttgcct ggtacttatc tggcctggtc ttcaaatggc
     3601 tgaaagcgaa cggcggtgtg gctgcaatgg ataagatcaa tcagcaaaaa gcagaactgc
     3661 tgtatggggt gattgataac agcgatttct accgcaatga tgtggcgaaa gctaaccgtt
     3721 cgcggatgaa cgtgccgttc cagttggcgg atagcgcgct tgataaattg ttcctcgaag
     3781 agtctttcgc tgcgggctta catgcgctga aaggtcaccg agtggtcggc ggaatgcgtg
     3841 cgtctattta taacgccatg ccgctggaag gtgttaaagc actgacagac ttcatggttg
     3901 agttcgaacg tcgtcacggc taatgtcgaa attttgcata atccccacag ccagcctgtg
     3961 gggtttttat ttctgttgta gagagttgag ttcatggaat ccctaacgtt acaacccatc
     4021 gctcgtgtcg atggcactat taatctgccc ggttccaaga gcgtttctaa ccgcgcttta
     4081 ttgctggcgg cattagcaca cggcaaaaca gtattaacca atctgctgga tagcgatgac
     4141 gtgcgccata tgctgaatgc attaacagcg ttaggggtaa gctatacgct ttcagccgat
     4201 cgtacgcgtt gcgaaattat cggtaacggt ggtccattac acgcagaaag tgcccgggag
     4261 ttgttcctcg gtaacgccgg aacggcaatg cgtccgctgg cggcagcact ttgcctgggt
     4321 agcaatgata ttgtactgac cggtgaaccg cgaatgaaag aacgcccgat tggtcatctg
     4381 gtggatgcgc tgcgccaggg cggggcgaag atcacttacc tggaacaaga aaattatccg
     4441 ccattgcgtt tacagggcgg ctttactggc ggcaacgttg atgttgatgg ctccgtttcc
     4501 agccagttcc tcaccgcact gttaatgact gcgcctcttg cgccggaaga tacggtgatt
     4561 cgtattaaag gcgatctggt ttctaaacct tatatcgata tcaccctcaa tctgatgaag
     4621 acgtttggtg ttgaaattga aaatcagcac tatcaacaat ttgtcgtaaa aggggggcag
     4681 tcttatcagt ctccgggtac ttatttggtc gaaggcgatg catcctcggc ttcttatttc
     4741 ctggcagcag cagcaatcag aggcggcact gtaaaagtga ccggcattgg acgcaacagt
     4801 atgcagggtg atattcgctt tgctgatgtg ctggaaaaaa tgggtgcgac catttgctgg
     4861 ggcgatgatt atatttcctg cacgcgtggt gaactgaacg ccattgatat ggatatgaac
     4921 catattcccg atgcggcgat gaccattgcc accgcggcgt tatttgcaaa aggcaccacc
     4981 acgctgcgca atatctataa ctggcgtgtt aaagagaccg atcgcctgtt tgcgatggca
     5041 acagaactgc gtaaagtcgg tgcggaagtc gaagaggggc acgattttat tcgcattacg
     5101 ccaccggaaa aactgaaatt tgctgagatc gccacttata atgatcaccg gatggcgatg
     5161 tgtttctcgc tggtcgcatt atcagatacg cctgtgacga ttcttgatcc aaaatgtacc
     5221 gccaaaactt ttccagacta tttcgagcaa ttggcgcgga taagtcaacc cggctgaatg
     5281 aataacgggc gataacatag acaatccttt cttttcaaag acgtcggcac attgccggcg
     5341 ttttttttcg gaccttgtgg ggtattttta ttaatggtag cgttaattat caatgtaact
     5401 tgttgataca taatgtttat atataattaa tcaacggatg attcacatga agaatactaa
     5461 attattgctg gcgatagcga cctctgcagc cttactgaca ggatgtcaga atacgcacgg
     5521 tattaatacc gatttggcta tcagctcagg tctgaatgct tataaagccg caacattaag
     5581 cgatgctgat gctaaagcaa tcgccaatca gggctgtgtc gaaatggaca gcgacaataa
     5641 agtggcaagc aaatccagca agtacgggaa acgtctggca aaaatcgcca aagcattggg
     5701 taacaatatt aatggcacgc cagtcaacta taaggtttat atgaccagcg acgtcaacgc
     5761 atgggcgatg gcgaacggtt gtgttcgtgt ctacagtggc ctgatggaca tgatgaacga
     5821 caacgaaatt gaaggcgttc tgggccatga actgggccac gtcgcgttgg gtcactcgct
     5881 ggctgaaatg aaagcttctt atgcgatcgt ggccgcacgc gatgccattt cagctaccag
     5941 cggtgtggct tcccagcttt cccgttcaca attaggtgat atcgcagaag gcgctatcaa
     6001 tgctaaatac tcccgtgata aagaatccga agcagatgat ttctcttttg atctgttgaa
     6061 gaaacgtggc atcagtaccc aggggctggt tggtagcttt gaaaaactgg ccagcctgga
     6121 tggcggtcgc acccagtcca tgtttgattc tcacccacca tcaacagagc gtgcgcaaca
     6181 catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg ctggtcttct
     6241 gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa ctggtttttg
     6301 cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta tgttaacggt
     6361 acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc attgatggcc
     6421 caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcgttg caatggcatc
     6481 tgctggattc gggtgcaatt tatcgcgtac tggcactagc ggcattacat caccatgttg
     6541 atgttgcatc ggaagatgcg ctggtgccgc tggcatccca tctggatgta cgttttgtgt
     6601 caaccaacgg taatctggaa gtgatcctcg aaggggaaga tgtcagcggc gaaattcgta
     6661 ctcaggaagt ggcgaatgct gcttcacaag tcgcggcatt cccacgcgtt cgtgaagcat
     6721 tattgcgtcg ccaacgcgcg tttcgcgaat taccaggcct gattgccgat ggccgcgaca
     6781 tgggaacggt ggtattccct gatgcgccag tgaaaatttt ccttgacgcc tcctcggaag
     6841 aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt aactttgagc
     6901 gccttttggc cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg gtagcgccac
     6961 tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt gagcaagtga
     7021 ttgaaaaagc gctacaatac gcgcgccaga aattggctct cgcataagcg accgaatttg
     7081 cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat ccggcatgga
     7141 gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt ttgctcaact
     7201 ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc gtggcgttgt
     7261 tgttgctatc gacaaagacg tagtactggt tgacgctggt ctgaaatctg agtccgccat
     7321 cccggctgag cagttcaaaa atgcccaggg cgaactggaa atccaggtag gtgacgaagt
     7381 tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt cccgtgagaa
     7441 agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg ctgaaactgt
     7501 taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga acggtattcg
     7561 tgcgttcctg ccaggttctc tggtagacgt tcgtccggtg cgtgacactc tgcacctgga
     7621 aggcaaagag cttgaattca aagtaatcaa gctggatcag aagcgcaaca acgttgttgt
     7681 ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc tgctggaaaa
     7741 cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact acggtgcatt
     7801 cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcct ggaaacgcgt
     7861 taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag tgctgaagtt
     7921 cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ctgggcgaag atccgtgggt
     7981 agctatcgct aaacgttatc cggaaggtac caaactgacc ggtcgcgtga ccaacctgac
     8041 cgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac acgtttccga
     8101 aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg gcgatgtagt
     8161 ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctgg gtctgaaaca
     8221 gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aacaagggcg accgtgttga
     8281 aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggatg gcggcatcga
     8341 cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag cagttcgtga
     8401 atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag aacgtgaacg
     8461 tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg ttgctctgaa
     8521 caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag gcgcaaccgt
     8581 agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc gtgaccgcgt
     8641 tgaagacgct accctggttc tgagcgttgg cgacgaagtt gaagctaaat tcaccggcgt
     8701 tgatcgtaaa aaccgcgcaa tcagcctgtc tgttcgtgcg aaagacgaag ctgacgagaa
     8761 agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca acgcaatggc
     8821 tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat ttttattccg
     8881 aagtttgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag cactaagggc
     8941 ggctacggcc gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc tttaaggaac
     9001 cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc aatcgcacat
     9061 tcccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg cctcgactct
     9121 tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc actaccgcgc
     9181 accacgtacc ggacgtaatc cgaagactgg cgataaagta gaactggaag gaaaatacgt
     9241 tcctcacttt aaaccaggta aagaactgcg cgatcgcgcc aatatttacg gttaagtttt
     9301 tactcaaact tgaacgcgag aaaagcacct gtcgggtgct tttttcattt ctctaatctg
     9361 gaactggaag ctgcctcgca gagttttgag cagttttcac cctatcgtta aattcttctg
     9421 aacgtgcctc ggggaacgca aaactcccac cagacaaccg ttcaacaaag ttgcacactt
     9481 tctataaaca gggagggtgc atgaaaataa cgacagtcgg cgtatgcata atttgcggaa
     9541 tttttccgtt gctgattttg ccccaattgc cagggacagt aacccttgcg tttctgactc
     9601 tcttcgcctg tgtactggca tttatccctg ttaaaaccgt ccgttatatc gcgctgacgt
     9661 tgctgttttt cgtttggggc atattagcgg caaagcaaat tttgtgggca ggagaaacct
     9721 taactggcgc gacgcaggat gcaattgttg agatcattgc tactgacggc atgaccactc
     9781 attacggtca aattactcat ctacaaggtc gacgtatatt ccctgcgcca ggcctcgtac
     9841 tgtatggcga atatcttccg caagcggttt gtgccggaca agtatggtca atgaaactca
     9901 aagttcgtgc agttcatggt caacttaatg atggcggctt tgatagccag cgttatgcca
     9961 ttgcccagca tcaaccgctc accggccgtt ttctgcaggc aagtgtcatt gaaccgaatt
    10021 gtagcttgcg tgcacagtat ctggcgtcac tacaaacaac gctgcaaccc tatatgtgga
    10081 acgcggttat tcttggatta ggtatggggg aacggttatc cgtccccaaa gaaatcaaaa
    10141 atatcatgcg tgatactgga acggcgcatt taatggcgat atcaggattg cacatcgctt
    10201 ttgcggcgtt gctggctgca ggactcattc gcggtgggca agtttttctg cctgggtgct
    10261 ggatccactg gcaaatgcca ttaattggtg gaatctgctg tgctgctttt tatgcctggc
    10321 ttacgggaat gcaacctcct gcattgcgta ccgtggtggc gcttgccatt tggggaatgc
    10381 ttaagttaag tggacgacaa tggagtggct gggatgtatg gatatgttgt ctggcggcaa
    10441 ttttgctgat ggatcctgtt gccattctct cgcaaagttt atggctatct gccgctgcgg
    10501 tcgcggcact gattttttgg tatcagtggt ttccctgtcc ggagtggcag ctgccgccgg
    10561 tattgcgcgc ggttgtttcc ctcatccatc tgcaactggg aatcaccctc ctgcttatgc
    10621 ccgtgcaaat tgtcatattt catggcatta gtctgacctc gtttattgca aatctttttg
    10681 caattcccct ggtgacattt atcacggttc cgttgattct cgccgcgatg gttgtgcatt
    10741 taagcgggcc gtttattctg gaacaagggt tatggtttct tgccgaccgg tctttggctt
    10801 tacttttctg ggggttaaag agtttgccgg aagggtggat caacattgct gaacgttggc
    10861 aatggctaac attttcccca tggttcttac tggtggtatg gcgattaaac gcctggcgaa
    10921 cgttgccagc aatgtgtgtg gctgtaggct tgctgatgtg cttgccgctg tggcaaaaac
    10981 ctcgacctga cgagtggcaa gtgtacatgc ttgatgtcgg gcaagggctg gcaatggtca
    11041 ttgcgcgaaa cggcaaagcg attctctatg acacgggact ggcctggccc gaaggggata
    11101 gtgggcaaca actgattatc ccctggctcc actggcataa tcttgaaccg gaaggcgtta
    11161 ttttgagtca tgaacatctg gatcaccggg gagggctgga ctcaatattg cacacatggc
    11221 cgatgttatg gatccgaagt ccgttaaact gggaacatca tcagccctgt gtgcgtggcg
    11281 aagcgtggca atggcaagga ttgcgtttca gcgcgcactg gcctttacaa ggtagcaacg
    11341 ataaaggaaa taaccattcc tgtgtggtta agattgatga cgggacgaat agcattcttc
    11401 taaccggtga tattgaagcc cccgctgaac aaaagatgct aagccgttac tggcagcaaa
    11461 tgcaggcaac attacttcag gtacctcacc atggcagtaa tacctcatca tcgttgccat
    11521 taattcagcg agtgaatgga aaagtggcac tcgcatcggc atcgcgctat aacgcatggc
    11581 gactgccctc aagcaaagtt aaacatcgct atcaacaaca gggttataag tggcttgata
    11641 ctccacatca gggtcaaata acggtcaatt tttcagcgca aggctggcgg attagcagcc
    11701 tcagagagca aattttacct cgttggtatc atcagtggtt tggcgtgcca gtggataacg
    11761 ggtagaatat gcggctattt caacaaatgc tggttttttg aatgcataac gacaaagatc
    11821 tctctacgtg gcagacgttc cgccgactgt ggccaaccat tgcgcctttt aaagcgggtc
    11881 tgatcgtggc gggcgtagcg ttaatcctca acgcagccag cgataccttc atgttatcgc
    11941 tccttaagcc acttcttgat gatggctttg gtaaaacaga tcgctccgtg ctgatgtgga
    12001 tgccgctggt ggtgatcggg ctgatgattt tacgtggtat caccagctat atctccagct
    12061 actgtatctc ctgggtatca ggaaaggtgg taatgactat gcgtcgccgc ctgtttggtc
    12121 acatgatggg aatgccggtt tcattctttg acaaacagtc aacgggtacg ctgttgtcac
    12181 gtatcaccta cgattccgaa caggtcgctt cttcttcttc cggcgcactg attactgttg
    12241 tgcgtgaagg tgcgtcgatc atcggcctgt tcatcatgat gttctattac agttggcaac
    12301 tgtcgatcat tttgattgtg ctggcaccga ttgtttcgat tgcgattcgc gttgtatcga
    12361 agcgttttcg caacatcagt aaaaacatgc agaacaccat ggggcaggtg accaccagcg
    12421 ccgaacaaat gctgaaaggc cataaagaag tattgatttt cggtggtcag gaagtggaaa
    12481 cgaaacgctt cgataaagtc agtaaccgaa tgcgtcttca ggggatgaaa atggtttcag
    12541 cctcttctat ctctgatccg atcattcagc tgatcgcctc tttggcgctg gcgtttgttc
    12601 tgtatgcagc aagcttccca agtgtcatgg atagcctgac tgccggtacg attaccgttg
    12661 ttttctcttc aatgattgca cttatgcgtc cgctgaaatc gctgaccaac gttaacgccc
    12721 agttccagcg tggtatggcg gcttgtcaga cgctgtttac cattctggac agtgagcagg
    12781 agaaagacga aggtaagcgc gtgatcgagc gtgcaactgg cgacgtggaa tttcgcaatg
    12841 tcacctttac ttatccagga cgtgacgtgc ccgcattgcg taacatcaac ctgaaaattc
    12901 cggcagggaa gacggttgct ctggttggac gatctggttc aggtaaatca accatcgcca
    12961 gcctaatcac acgtttttac gatattgatg aaggcgaaat cctgatggat ggtcacgatc
    13021 tgcgcgagta taccctggcg tcattacgta accaggttgc tctggtgtcg cagaatgtcc
    13081 atctgtttaa cgatacggtt gccaacaaca ttgcttacgc aaggactgaa cagtacagcc
    13141 gtgagcaaat tgaagaagct gcgcgtatgg cctacgccat ggacttcatc aataagatgg
    13201 ataacggtct cgatacagtc attggtgaaa acggcgtgtt gctctctggc ggtcagcgtc
    13261 agcgtattgc tatcgctcga gccttgttgc gtgatagccc gatactgatt ctggacgaag
    13321 ctacctcggc gttggatacc gaatccgaac gtgcgattca ggcggcactg gatgagttgc
    13381 agaaaaaccg tacctctctg gtgattgccc accgcttgtc taccattgaa aaggcagacg
    13441 aaatcgtggt tgtcgaggat ggtgtcattg tggaacgcgg tacgcataac gatttgcttg
    13501 agcaccgtgg cgtttacgcg caacttcaca aaatgcagtt tggccaatga tcgaaaaaat
    13561 ctggtctggt gaatcccctt tatggcggct attgctgcca ctctcctggt tgtatggcct
    13621 ggtgagtggc gcgatccgtc tttgctataa actaaaactg aagcgcgcct ggcgtgcccc
    13681 cataccggtt gtcgtggttg gtaatctcac cgcaggcggc aacggaaaaa ccccggtcgt
    13741 tgtctggctg gtggaacagc tgcaacagcg cggtattcgc gtgggggttg tatcgcgggg
    13801 atatggtggt aaggctgaat cttatccgct gttattgtcg gcagatacca ccacagcaca
    13861 ggcgggtgat gaacctgtgt tgatttatca acgcactggc gcacctgttg cggtttctcc
    13921 cgtgcgttct gacgcggtaa atgccattct ggcgcaacac cctgatgtgc agatcatcgt
    13981 aaccgacgat ggtttacagc attatcgtct ggcgcgtgat gtggaaattg tcgttattga
    14041 tggtgtgcgt cgctttggca atggctggtg gttgccggcg gggccaatgc gtgagcgagc
    14101 ggggcgctta aagtcggttg atgcggtaat cgtcaacggc ggtgtccccc gcagcggtga
    14161 aatccccatg catctgctgc cgggtcaggc ggtgaattta cgtaccggta ctcgttgtga
    14221 cgttgctcag cttgaacatg tagtggcgat ggcggggatt ggacatccgc cgcgcttttt
    14281 tgccacgctg aagatgtgtg gcgtacaacc ggaaaaatgt gtaccgctgg ccgatcatca
    14341 gtctttgaat catgcggatg tcagcgcgtt ggtaagcgcc gggcaaacgc tggtaatgac
    14401 tgaaaaagat gcggtgaaat gccgggcctt tgcagaagaa aattggtggt atttgcctgt
    14461 agacgtacag ctttcaggtg atgaaccagc gaaactgctt gcgcaactaa cctcgctggc
    14521 ttctggcaac tagttacgcc gcggcagcgt tcgattgatg gagtcatgaa tgtcgctgcc
    14581 gcacctctct cttgctgatg cgcgtaatct tcacctcgcc gcgcaaggcc tgttaaacaa
    14641 accccgccgt cgagcgtcgt tggaggatat tccggcaacg atctcccgca tgtccttgct
    14701 gcaaatcgat accattaata ttgttgcccg tagcccatat ctggtgcttt tcagtcgtct
    14761 gggaaattat cctgcccagt ggctggatga gtctctggcg cgtggcgaat taatggaata
    14821 ctgggcgcat gaagcctgct ttatgccgcg tagcgatttt cgtcttattc gccaccgcat
    14881 gctggcacct gaaaaaatgg gctggaaata caaagacgcc tggatgcagg aacatgcggc
    14941 ggaaattgca cagttaattc agcatattca tgataagggg ccggtacgtt cagccgattt
    15001 cgagcatcct cgtaaagggg cgagcggctg gtgggagtgg aagccgcata aacgacatct
    15061 ggaaggttta tttactgccg gaaaggtgat ggtgattgaa cggcgcaact tccagcgcgt
    15121 ttatgattta actcaccgtg tcatgcctga ctgggatgat gagcgcgatc tcgtttcgca
    15181 agcagaagca gaaatcatca tgctggataa cagtgcgcgt agcctgggca tattccgcga
    15241 acagtggctg gcagattact atcggctgaa acgtccggca ctggcggctt ggcgcgaagc
    15301 gagggctgaa ctgcagcaaa tcattgctgt gcatgttgaa aaattgggca atctttggct
    15361 gcatgctgat ttgctgccgc tactcgagcg tgcgctcgcc ggaaagctca ctgcaacgca
    15421 cagcgcggta ctttcgcctt ttgatcctgt tgtctgggat cgcaaacgcg cagagcagct
    15481 ttttgatttt agctaccggc tggagtgcta taccccagcg ccgaaacgcc agtatggcta
    15541 ttttgttctg ccgttattac atcgtgggca attagttggg cgaatggatg ccaaaatgca
    15601 tcgccagaca ggcatccttg aagttatctc tctgtggtta caggaaggca ttaaaccaac
    15661 gacaacgctg caaaaagggt tacgtcaggc gattactgat ttcgctaact ggcagcaggc
    15721 aacgcgggtg acattaggac gctgcccgca aggcctcttt actgattgcc gcaccggctg
    15781 ggaaatagac cccgtcgcat aaatgattat gataagattt atgcattcat aacccgatcc
    15841 atctggagga actatggatc atcgtctgct tgaaatcatt gcctgcccgg tttgcaacgg
    15901 aaaactttgg tataaccagg aaaaacaaga acttatttgc aaactggaca acctggcttt
    15961 cccgctgcgc gatggcattc cagtgttgct ggaaacggaa gcccgcgtgc tgactgctga
    16021 tgagagtaaa tcatgagttt tgtggtcatt attcccgcgc gctacgcgtc cacgcgtctg
    16081 cccggtaaac cattggttga tattaacggc aaacccatga ttgttcatgt tcttgaacgc
    16141 gcgcgcgaat caggtgccga gcgcatcatc gtggcaaccg atcatgagga tgttgcccgc
    16201 gctgttgaag ccgctggcgg tgaagtatgt atgacgcgcg ccgatcatca gtcaggaaca
    16261 gaacgtctgg cggaagttgt cgaaaaatgc gcattcagcg acgacacggt gatcgttaat
    16321 gtgcaggggg atgaaccgat gatccctgcg acaatcattc gtcaggttgc tgataatctc
    16381 gcccagcgtc aggtgggtat ggcgactctg gcggtgccaa tccacaatgc ggaggaagcg
    16441 tttaacccga atgtggtgaa agtggtcctc gacgctgaag ggtatgcact gtacttctct
    16501 cgcgccacca ttccttggga tcgtgatcgt tttgcaaaag gccttgaaac cgttggcgat
    16561 aacttcctgc gtcatcttgg tatttatggc taccgtgcag gctttatccg tcgttacgtc
    16621 aactggcagc caagtccgtt agaacacatc gaaatgttag agcagcttcg tgttctgtgg
    16681 tacggcgaaa aaatccatgt tgctgtcgcc caggaagttc ctggcacagg tgtggatacc
    16741 cctgaagatc tcgagcgcgt tcgcgctgaa atgcgctaat ttcacttcac gacactccag
    16801 ccaattttgg gaggagtgtc gtaccgttac gattttcctc aatttttctt ttcaacaatt
    16861 gatctcattc aggtgacatc ttttatattg gcgctcatta tgaaagcagt agcttttatg
    16921 agggtaatct gaatggaaca gctgcgtgcc gaattaagcc atttactggg cgaaaaactc
    16981 agtcgtattg agtgcgtcaa tgaaaaagcg gatacggcgt tgtgggcttt gtatgacagc
    17041 cagggaaacc caatgccgtt aatggcaaga agctttagta cgcccggaaa agcccgacaa
    17101 ctggcatgga aaaccaccat gctggcaaga agtgggactg tccgtatgcc gactatttat
    17161 ggtgtgatga cgcatgaaga acaccccggc cccgatgtcc tgctactgga gcgaatgcgt
    17221 ggtgtttcgg tggaggcacc agcccgaaca ccagaacgct gggaacaact caaagatcaa
    17281 atcgttgaag ccttactggc ctggcaccgt caggacagtc gcggttgcgt cggcgcggtc
    17341 gacaatactc aggaaaattt ctggccctca tggtaccggc aacatgttga agtgctatgg
    17401 accacgctca atcagttcaa taacaccggt ctaacgatgc aggataagcg gatcctgttt
    17461 cgcactcgcg aatgtctccc ggcattattt gaaggcttta acgacaattg tgtactgatt
    17521 cacggtaact tctgtttacg tagcatgctg aaagattcgc gcagcgatca gttactggcg
    17581 atggtcggac cgggactaat gctttgggca ccgcgagaat acgaactgtt ccgactaatg
    17641 gataattctc tggcggaaga tttgctctgg ggttatctgc aacgcgcgcc agtggcggag
    17701 tcgttcatct ggcggcgttg gttgtatgtg ttatgggatg aagttgcgca actggtcaac
    17761 accggacgat ttagtcggcg taacttcgat ctggcatcaa aatcactctt gccgtggctc
    17821 gcctgacgaa ccttttagcc actgccagat gcggccaagc gtttcatagc caacacgatc
    17881 gctatgcatc aaccaaaccg gagaagggat tgcccgttcc cacggattaa gcggcgagtc
    17941 gatggccaac tgattcgccg gggctggcag cggatttagc ccttcctgct gaaaaaagat
    18001 catcgcccgc ggcagatgtg atgcggaggt caccagcagg aaaggggcgt caccaattgc
    18061 ctgtttcact gccgcagctt cttcttcggt atcttttggc aaatccagag tgataatttg
    18121 ctcgcgcggc acgcccagcg attgcgcaac tcttgcacct acttcggctg tactcaccgt
    18181 attggttttt gctacgcctc ccgtgaagat cagttttgat cccggatttt cgcgccataa
    18241 gcgaatacct tcattcaggc ggggcagact gttattgatt aaattcgagc ttggtgccca
    18301 ctgcgggttc caggtataac cacctccgag caccacgata tagtccactt tttgcgaatt
    18361 attccatgtc ggataggtgc tttcgatggg acgcagcagg cggtcggcta ccggttgcaa
    18421 actcaataac aagagcgcca gccacccaat gctaataaaa atcttcccgg ttttttgaaa
    18481 acggctaaac cacagcagcg ccaggccagc gccaataatc aacaacatca gcggaagtgg
    18541 cagcaacata ttgccaatca cttttttcag tgtaaaaagc atcctttctg gttccttttt
    18601 tgaccatata gcaagggatc gccagcgata ttacaccaga cgggttcatt ctcgccgtgg
    18661 ctgtgacaaa atagcgtctt tatcagaaaa cgtcttagcc aaccggtgga gaaagcaatg
    18721 caggatcgca attttgatga tattgcggaa aagttttccc gtaacattta cggcaccacc
    18781 aaagggcagc ttcgacaggc tattctgtgg caggatctcg atcgcgtgct ggcggaaatg
    18841 ggcccgcaaa aactgcgtgt gctggatgct ggcggtggag aagggcagac cgcaatcaaa
    18901 atggccgagc gcgggcatca ggtcatttta tgcgatcttt ctgcgcagat gatcgaccgc
    18961 gcaaaacagg cggcagaagc aaaaggtgtg agcgacaaca tgcaatttat acattgcgcc
    19021 gctcaggatg ttgcttcgca tttggaaacg cccgttgatc tgatattgtt ccatgcggtg
    19081 ctcgagtggg tggccgatcc ccgcagcgta ttacagaccc tgtggtcagt gttgcgtcca
    19141 ggcggcgtgt tgtcgttaat gttctacaat gcgcatggtt tgttgatgca taacatggtc
    19201 gccgggaatt ttgattacgt gcaggcggga atgccgaaaa agaaaaaacg gacgctttcg
    19261 ccagattatc cacgcgaccc ggcgcaggtt tatctgtggc tggaagaagc tggttggcaa
    19321 attctgggta agacaggcgt tcgcgtgttt catgattatc tgcgcgagaa gcaccagcag
    19381 cgcgactgct atgaagcatt acttgaatta gaaacgcgtt attgccgtca ggaaccgtat
    19441 attaccctgg ggcgttatat tcatgtcacc gcgcgcaaac cgcagagcaa ggataaagta
    19501 tgagtgaatt ttcccagaca gtccccgaac tggttgcctg ggccagaaaa aatgacttct
    19561 ccatctcgct gccggtagac cgactctctt ttctgctggc ggttgccacg ctgaacggcg
    19621 agcgtctgga tggtgagatg agtgaaggcg agctggtgga tgcattccgc catgtgagtg
    19681 atgcgtttga gcaaaccagc gaaaccatcg gcgtgcgcgc caacaacgcg atcaacgaca
    19741 tggtgcgtca acgtctgctg aaccgcttta ccagcgagca ggcggaaggg aacgcaattt
    19801 accgtctgac gccgctcggc atcggcatca ctgactatta cattcgtcag cgcgagtttt
    19861 ctacgctgcg cctttctatg caattgtcga ttgtggcggg tgagctcaaa cgcgcagcag
    19921 atgccgccga agagggcggt gatgaatttc actggcaccg taatgtttat gcgccactga
    19981 aatattcggt agcagaaatt ttcgacagta tcgacctgac gcaacgtctg atggatgaac
    20041 agcagcagca ggtgaaggat gatatcgccc agttgttgaa caaagactgg cgggcggcga
    20101 tttccagctg tgaattgttg ctttcagaaa cttccggaac gctgcgtgaa ttgcaggata
    20161 cgctggaagc ggcaggcgat aaattgcagg ccaacctgtt acgcattcag gatgcgacga
    20221 tgacccatga cgacctgcat tttgtcgatc gtctggtgtt cgatcttcag agcaaactcg
    20281 accgtattat cagttggggc cagcaatcca tcgacttgtg gattggctac gaccgccacg
    20341 tacacaaatt tattcgtact gcgatcgata tggataaaaa ccgcgtcttt gctcagcggt
    20401 tacgtcagtc ggtacaaacc tattttgatg agccgtgggc gctaacttat gccaatgccg
    20461 atcgtctgct ggatatgcgt gacgaagaga tggtactgcg cgatgaagaa gtgactgggg
    20521 aacttcctga ggatctggag tacgaagagt ttaacgagat ccgcgaacag ctggcggcga
    20581 tcatcgaaga acaacttgcc gtgtacaaaa ccagacaagt gccgttggat cttggtctgg
    20641 tggtacgcga atatctgtca cagtatccgc gtgcacgtca ctttgacgtt gcgcgcattg
    20701 ttattgatca ggcggtacgt cttggcgtag cgcaagcaga tttcaccgga ctgccagcga
    20761 aatggcagcc gattaatgat tacggagcca aggtacaggc gcatgtcatc gacaaatatt
    20821 gaacaagtga tgccggttaa gctggcgcag gcgctggcga atccgttatt tccggcgctg
    20881 gatagcgcct tacgttcagg ccgtcatatt ggcctcgacg aactggataa tcatgcattc
    20941 ctgatggatt ttcaggaata tctggaagag ttttacgcgc gttataacgt tgagcttatt
    21001 cgtgcaccgg aagggttctt ctatctacgc ccacgttcca ccacgctgat ccctcgttcc
    21061 gtcttgtcgg aactggatat gatggtcggg aaaatcctct gttatctcta tctcagcccg
    21121 gaacggctgg cgaatgaggg gatttttact caacaggaac tgtacgacga actgctcacc
    21181 ctggccgatg aagcgaaact gctgaaactg gtgaacaacc gttcaaccgg ttcagacgtt
    21241 gaccgtcaga agttgcagga aaaagtacgt tcttcgctca accgtctgcg tcgtttaggc
    21301 atggtgtggt ttatgggcca cgacagcagc aagttccgca ttaccgaatc ggtgttccgc
    21361 ttcggagccg atgtgcgtgc tggcgacgat ccccgtgaag cacagcgtcg cctgattcgt
    21421 gatggtgaag caatgccgat tgaaaatcat ctgcaactca acgatgaaac cgaagagagt
    21481 cagccagata gcggagagga agaataatga ttgaacgcgg taaatttcgc tcactgacgc
    21541 tgattaactg gaacggcttt tttgcccgaa cttttgacct tgacgagctg gtcacgacgc
    21601 tttctggcgg taacggggcg ggtaaatcta ccaccatggc ggcgttcgtt acggcgctta
    21661 tccccgacct gaccctgctg catttccgta acactacgga agccggggcc accagcggtt
    21721 cgcgcgataa aggtctgcac ggtaagctga aagcgggtgt ctgttattcg atgctcgaca
    21781 ccattaactc gcgccaccag cgcgtagtgg tcggtgtgcg cctgcaacag gttgccggac
    21841 gcgatcgtaa agtggatatc aaaccatttg ccattcaggg cctgccgatg tcggtgcagc
    21901 cgacacaact ggtgaccgaa actctgaacg aacgccaggc gcgcgtgctg ccgcttaatg
    21961 agctgaaaga caagctcgag gcgatggaag gcgtgcagtt taagcagttc aactccatta
    22021 ccgattacca ctcgctgatg ttcgatctgg gcatcatcgc gcgtcgtctg cgctcagcgt
    22081 ctgaccgtag taaattctat cgtctgatcg aagcctcgct gtatggcggg atctccagcg
    22141 ccattactcg ttctctgcgc gactacctgt tgccggaaaa cagtggggtg cggaaagcgt
    22201 tccaggacat ggaagcggcg ctgcgtgaaa accgcatgac gctggaagcg attcgtgtca
    22261 cccagtcgga ccgtgacctg tttaagcatc tgatcagcga agccaccaac tacgtggcgg
    22321 cggactacat gcgtcacgcc aacgagcgcc gtgttcatct cgataaggcc ctggagtttc
    22381 gtcgcgagct acatacttcg cgtcagcaac tggcggctga gcagtacaaa cacgtcgata
    22441 tggcgcgaga gctggctgag cacaacggtg ccgaaggcga tctggaagcg gattatcagg
    22501 cggccagcga tcacctgaac ctggtgcaaa ccgcactgcg tcagcaggag aagatcgaac
    22561 gctacgaagc ggatctcgat gagctgcaga tccgtctgga agagcaaaac gaagtggtgg
    22621 cagaagccat cgaacgccag gaagagaacg aggctcgtgc ggaagctgcc gaactggaag
    22681 tggacgagct gaaaagccag cttgctgact accagcaggc gctggacgtc cagcaaacgc
    22741 gcgcgatcca gtataaccag gcgattgctg cgcttaatcg tgccaaagaa ctgtgccatc
    22801 tgccggactt aaccgccgac agcgctgccg aatggctgga aaccttccag gcgaaagagc
    22861 tggaagcgac cgaaaaaatg ctctctcttg agcagaaaat gagcatggcg caaaccgcgc
    22921 acagccagtt tgagcaggct tatcagctgg tggtggcaat caacggcccg ctggcgcgta
    22981 acgaggcgtg ggatgtcgct cgcgaactat tgcgcgaagg ggtcgatcag cgtcacctgg
    23041 cagagcaggt tcagccgttg cggatgcgct taagcgaact ggaacagcgt ctgcgtgagc
    23101 agcaagaagc tgagcgtctg ctggcagatt tctgcaaacg tcagggcaag aattttgata
    23161 ttgatgaact ggaagctctg catcaggagc tggaagcacg cattgcctct ctttccgata
    23221 gcgtctctaa cgcccgagaa gagcggatgg cactgcgcca ggagcaggaa cagctgcagt
    23281 ctcgcattca gagtttgatg cagcgtgcgc cggtttggct ggcagcgcaa aacagtctca
    23341 accagttgag cgaacagtgc ggcgaagagt tcacctccag ccaggatgtc accgaatttc
    23401 tgcaacagtt gctggagcgt gagcgcgagg caattgttga acgcgatgaa gtaggcgcgc
    23461 gcaaaaacgc cgtcgatgaa gagatcgaac gtttaagcca gccaggtggt tctgaagatc
    23521 agcgtctgaa cgcgctggcg gagcgttttg gtggtgtgct gctgtcagaa atttatgacg
    23581 acgtaagcct ggaagatgcg ccgtacttct cagcgctgta tggcccgtca cgccacgcca
    23641 tcgtggtgcc agatctgtca caggtaaccg aacacctgga aggcttgacc gattgcccgg
    23701 aagatctcta tttgatcgaa ggggatccgc agtcattcga tgacagcgtg ttcagcgttg
    23761 atgagctgga aaaagcggtg gtggtgaaaa tcgccgatcg tcagtggcgt tattcacgtt
    23821 tcccggaagt gccgctgttt ggtcgtgctg cgcgtgaaag ccgtattgaa agcctccatg
    23881 ccgagcgtga agtgctttcc gaacgcttcg ccacgctctc ctttgatgta cagaaaaccc
    23941 agcgtctgca tcaggcgttc agccgcttta tcggcagtca tctggcggtg gcgtttgagt
    24001 ctgacccgga agcagaaatc cgtcaactga acagccgtcg cgtcgaactg gagcgggcgt
    24061 tgagtaatca tgaaaatgat aaccagcagc agcgtattca gtttgagcag gcgaaagagg
    24121 gcgttacggc gctgaaccgc attctgccgc gtctcaacct gttggctgat gacagcctgg
    24181 cggatcgcgt cgatgaaatc cgcgaacgtc tggatgaagc ccaggaagcc gcgcgttttg
    24241 ttcagcagtt tggcaatcaa ctggcgaaac tggaaccgat cgtttcggta ttgcagagcg
    24301 acccggaaca gttcgaacag ttaaaagaag attacgcgta ctctcagcag atgcagcgcg
    24361 atgcccgtca gcaggcgttt gccctgacgg aagtggtaca gcgtcgtgcg cactttagct
    24421 actctgactc ggcagaaatg cttagcggta acagcgatct caacgaaaaa ctgcgtgaac
    24481 gtctggaaca ggcggaagcg gagcgtaccc gcgctcgtga agcgttgcgc ggacacgcag
    24541 cgcagttgaa tcagtacaac caggtgctgg cttcgctgaa aagttcgtat gacaccaaaa
    24601 aagagctact taacgatctg caacgtgaat tgcaggatat cggcgtgcgt gctgatagcg
    24661 gggcagaaga gcgggcacgt attcgccgtg acgagttgca cgcgcaactg agcaataacc
    24721 gttcacgacg caatcaactg gaaaaagcgc ttaccttctg cgaagcggag atggacaacc
    24781 tgacccgtaa actgcgcaag ctggagcggg attactttga gatgcgcgag caggtagtga
    24841 ccgccaaagc gggctggtgt gcggtgatgc gcatggtgaa agataatggc gttgagcgtc
    24901 gcttacaccg tcgtgagctg gcttatctct ccgctgatga tttgcgttcc atgtcggata
    24961 aggcgttagg tgcgctgcgt ctggcggtgg cagataacga acatctgcgc gacgtgctgc
    25021 ggatgtcgga agatccaaaa cgtccggaac gtaaaattca gttcttcgtg gcggtttatc
    25081 agcatctgcg tgaacgtatt cgtcaggata ttattcgtac cgatgatccg gtggaagcta
    25141 tcgaacagat ggagatcgag cttagccgtc tgaccgaaga attaacctcc cgtgaacaga
    25201 aactggcgat cagttcccgc agcgtggcga acatcattcg caaaaccatt cagcgcgagc
    25261 agaaccgtat ccgtatgctc aaccaggggt tgcagaacgt atcgtttggc caggtgaaca
    25321 gcgtacgtct gaacgtgaac gtacgtgaaa cgcacgccat gctgctggat gtgctctctg
    25381 aacagcacga gcagcatcag gatctgttta acagcaaccg cctgaccttc tcagaagcgc
    25441 tggcgaaact gtatcaacgt cttaacccgc agattgatat ggggcagcgc acgccgcaga
    25501 ccatcggtga agaactgctg gattaccgca actatctgga aatggaagtt gaggttaacc
    25561 gtggttccga tggctggttg cgtgcagagt ctggtgcatt gtcgaccggt gaggcgattg
    25621 gtaccggtat gtcgattctg gtgatggtgg tacaaagctg ggaagatgaa tctcgccgcc
    25681 tgcgcggtaa agatatctct ccttgccgcc tgctgttcct cgatgaagca gcgcgactgg
    25741 acgcccgttc catcgccacg ctgtttgaat tgtgtgaacg tttgcaaatg cagctcatta
    25801 tcgcagcgcc ggaaaatatc agcccggaga aaggcaccac ctataaactg gtgcgtaaag
    25861 tcttccagaa taccgaacac gttcacgtcg tcggcctgcg aggatttgcg cctcaactcc
    25921 ctgaaacgct tccaggaagt gacgaagcgc cttctcaggc gagttaaaga aaaggcggca
    25981 gcaatgccgc cttttctttt tccgaaaact ccgtttctgc actaaaaaag tggcacatta
    26041 cggcgcgctt ttctttaaac ttctttacat taggttatgt aaaaacgtat cggcgtttat
    26101 atactgaaga taagtctgat gagtaacagg cttgctcgtc atactttcgt gagtattggc
    26161 gttgtacagg caagtcgtaa aataacagcc tggctattca gagtatgata aaaacagggg
    26221 gcaagggatg ttgcttaata tgatgtgtgg tcgtcggctg tcggcaatca gtttgtgcct
    26281 ggccgtaaca ttcgctccac tgttcaatgc gcaggccgat gagcctgaag taatccctgg
    26341 cgacagcccg gtggctgtca gtgaacaggg cgaggcactg ccgcaggcgc aagccacggc
    26401 aataatggcg gggatccaac cattgcctga aggtgcggct gaaaaagccc gcacgcaaat
    26461 cgaatctcaa ttacccgcag gttataagcc ggtttatctt aaccagcttc aattgttgta
    26521 tgccgcacgc gatatgcaac ccatgtggga aaaccgtgat gctgttaaag ccttccagca
    26581 acagctggca gaggtggcga ttgccggttt ccagccgcag tttaataaat gggtagagtt
    26641 actgaccgat cctggtgtta acgggatgac acgcgacgtg gtgctctctg atgcgatgat
    26701 gggctatctc catttcattg caaatattcc ggtcaaaggc actcgctggc tatatagcag
    26761 taaaccttat gcgcttgcaa cgccgccgct ttcggtgatt aaccaatggc agctggcgct
    26821 ggataaaggt caattgccta cgtttgttgc aggactggca ccgcagcatc cgcaatatgc
    26881 ggcgatgcat gaatcgttac tggccttact cagtgacacc aaaccgtggc cccaactgac
    26941 cggcaaagca acgttgcgcc caggacagtg gagtaacgac gtaccggcgt tgcgcgaaat
    27001 attgcaacgt acaggcatgt tggacggggg accgaaaatt acgctacctg gcgatgacac
    27061 gccaactgac gcggtagtca gcccatccgc tgttactatt gaaacagcag aaactaagcc
    27121 gatggacaag caaactacgt ctcgtagtaa acctgcgcct gtcgttcgtg ccgcctacga
    27181 taatgaactg gtggaagccg ttaaacgttt tcaggcatgg caaggattgg gggcagatgg
    27241 tgctattggc ccggcaacgc gtgactggtt aaacgtaacg cccgcccagc gtgctggcgt
    27301 gttggcactc aacatccagc gattgcgctt gctgccaaca gagctttcta ccgggatcat
    27361 ggttaacatt ccggcctatt cgctggtcta ctatcagaac ggcaatcagg tgctggattc
    27421 gcgagtcatt gtcggtcgcc ccgatcgcaa aacgccaatg atgagcagtg cccttaacaa
    27481 cgtagtggta aacccgccgt ggaacgtccc accaactctg gcacgcaaag atattctgcc
    27541 gaaagtgcgc aacgatccgg gatatctcga aagccatggc tatacggtga tgcgcggctg
    27601 gaacaacaga gaagcgattg acccatggca ggttgactgg tctacaatca cggcctcgaa
    27661 tttaccgttc cgcttccagc aggctccagg cccacggaac tcgctggggc gctataaatt
    27721 caatatgccg agttcagagg ccatttattt gcatgacacg ccgaaccaca atctgttcaa
    27781 gcgtgataca cgcgcattga gctcaggctg tgtacgagtg aataaagctt ccgatctggc
    27841 gaatatgctg ttgcaggatg caggctggaa tgacaaacgt atttctgatg cgctgaagca
    27901 gggcgatacg cgttacgtca atattcggca gtcgattccg gtgaatctct actatctgac
    27961 ggcctttgtt ggtgcagatg gtcgtaccca gtatcgtaca gatatttaca attatgatct
    28021 gcctgcgcga tccagctcgc aaatcgtatc gaaagcggaa caattaatca ggtaaatgaa
    28081 gcagttcggg gaaatcagtt gtcgtaataa ttagcatgat cgggggcgat tctctgcagc
    28141 ccccgtcact gctggggttg agtcatcttg acgtctgctt tacgggcggt taaggtgcct
    28201 cttgtgcgcc agaagtgcat ataaacgata acattgacct gtagacttga ttatcatgga
    28261 caaattcgac gctaatcgcc gcaaactgct ggcgcttggt ggcgttgcac tcggtgccgc
    28321 catcctgccg accccggcgt ttgcaacact ctctacccca cgcccgcgca ttttgacact
    28381 caataatctt cataccggag agtcaatcaa agcggagttt ttcgatggca gaggctatat
    28441 tcaggaagaa ttggcaaaac ttaaccattt tttccgcgat taccgcgcga acaaaataaa
    28501 gtccatcgac ccaggattat tcgaccagtt gtatcgcctg caagggttgt taggcacccg
    28561 caaaccggtg caactcattt ccggttatcg ttccattgat accaacaatg aactacgcgc
    28621 ccgcagccgt ggagtagcga agaaaagcta tcacactaaa ggccaggcga tggatttcca
    28681 cattgaaggt atcgcgttaa gcaatattcg caaagccgcg ttatctatgc gcgcaggtgg
    28741 tgtaggatat tacccacgta gtaactttgt gcatattgat accgggccag cacggcactg
    28801 gtagtaatcg cttaacgaaa caggggcagt atgaactatc gtattattcc ggtcaccgca
    28861 ttctcccaga actgttcatt gatctggtgt gaacaaaccc gtctggccgc actggtcgat
    28921 cctggcggcg atgcggaaaa aatcaaacag gaagttgatg ccagcggcct gacactgatg
    28981 cagatcctgc tgactcatgg tcatctggac cacgttggcg cagcggcgga actggcgcaa
    29041 cattacggcg tgccggtttt tggcccggaa aaagaagatg agttctggct gcaaggcttg
    29101 cctgcgcaaa gtcgtatgtt tggtctggaa gagtgccagc cgctgacgcc agatcgttgg
    29161 ctgaacgaag gcgataccat cagcataggg aatgtgactt tacaggtgtt acattgccct
    29221 gggcatacgc cgggtcatgt cgtgtttttt gatgatcggg caaagctgct gatttctggc
    29281 gacgttattt tcaaaggtgg agtagggcgc agtgacttcc cgcgtggcga tcataatcaa
    29341 ctgatttctt caatcaaaga taaattgctg ccactggggg atgacgtgac atttattccg
    29401 ggtcacggac cattatccac acttggttat gagcgcctgc ataatccctt cctgcaagac
    29461 gaaatgcccg tctggtaaag gcgcataaaa aagcccgctt tcaagcgggc ttcatcggtt
    29521 ttaacgctta cagcactgcc acaatcgctt cgcacagcgg cgccatgtta tctggtgtca
    29581 tcccggccac gtttacgcga ccagaagcaa cagcatatac gccaaactct tcgcgcagac
    29641 gcagcacttg ttcttttgtc aggccactga aggagaacat gccgttctgt ttgatgataa
    29701 agctgaagtc gcgatttgcg cctttttcct gcagcgtatt gacgaacaac tgacgcatac
    29761 gctgaatacg ctggcgcata tcagtcagtt cttgttccca aatcgcacgt aacgcatcgt
    29821 tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg tgggttagag tagttagcac
    29881 gaatcgccgc tttcatttgg ctgaatgcgc gttcaacagt ttcactgtca gcagcaacca
    29941 gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt tttagagtag gaactggcaa
    30001 caatcagctc cttatgcaga gccgcgaaag cgcgcagtcc ttcagcatct tcttccagac
    30061 cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa ccagcctttc tcaacggaga
    30121 gttgtgccag tgtctgccat tgttccagcg tagggtcgat gccggttggg ttatggcagc
    30181 agccatggaa cagcactacg tcgccagcct gagcttcgtt caggctgtta atcagtgcat
    30241 cgaagtcaag agtgtgattt tccgcatcat aataagcgta ttcacgaact tccagacctg
    30301 cagagttaaa gacgctctta tggttcggcc agcttgggtt gctcacccac acacgcttaa
    30361 cgctggtatt ttttaccagg aaatcggcag ccacgcgtag tgcgccagtg ccacccggag
    30421 tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct acctttacca aacagcagtt
    30481 cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc gaggtaattt ttggtggttt
    30541 cattttcgag cagatactgt tcagcctttt tcacgctggt cagtaccggg gttttacccg
    30601 tctcatcttt atagacacca atcccgaggt taattttgcc gggacgttca tcggcacgaa
    30661 acagatcggc caggcccaga atcgggtcgg caggagcggc ggtaatgttc tcaaacatga
    30721 cgaggttcca ttatggttac agaagggaag tccgctatca gggtaacggg agatttacaa
    30781 aattccaact attactgatg aaaacgcagg ctgtttttgc aagacgtgag attgctctgg
    30841 aaggcataaa aaaaacagga ccaaagtcct gttttttcgg catttaacaa agaggtgtgc
    30901 tattagaact ggtaaacgat acccacagca acggtgtcgt cagaacctac gcccagtttg
    30961 ttgtcagaat cgatctggtt gatgatgtag tcaacatagg tggacatgtt tttgttgaaa
    31021 tagtaggttg cgcccacttc aaagtagttc accagatcaa catcaccgat accttctacg
    31081 tctttcgctt tagatttggt gtaagcgatg gacggacgca gaccgaaatc aaactggtat
    31141 tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc cgctggtgtt tgtaaattta
    31201 ttagtgatcg gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta gatgttgttc
    31261 gcgtcatact tcagaccagt agcccactgt tcagcttttt tgcctttgcc aagggaagac
    31321 tcttcctgca ggttggtacg gtcagcggca ccataagcac caacgatacc aaagccttcg
    31381 tattcgtagc tgatagaacc gccaacaccg tcgccgttag agcggcgtgc agtgtcacgc
    31441 tcgtttttac ccaggtactg aacagcgaag ttcagaccat caaccagacc aaagaagttg
    31501 gagttacgat aggtagcaac gccgccaaca cgaccaacga agaaatcatc gctgtatgca
    31561 gtatcaccac caaattctgg cagcatatcg gtgtaaccca gtgcatcata aaccacaccg
    31621 tagttacggc cgtaatcgaa agaaccaacg tcagcgtatt taagacccgc gaatgccaga
    31681 cgcgttttgt taccagtttg agcgtcagcg ccctcagagt tgttaccctg gaagttatat
    31741 tcccactgac cataaccggt cagatcggaa ttgatttgag tttccccttt aaaaccaaga
    31801 cgggcatagg tcatgtcgcc attgccaccg taactgtttt caccgttacc cttggagaaa
    31861 taatgcagac cgacagcttt accgtacaga tctactttgt tgccatcttt gttatagatt
    31921 tctgcagcgt ttgcagtacc tgctactaac agagcaggga cgatcactgc cagaatattg
    31981 cgcttcatca ttatttatta ccctcatggt ttttttatga cacctgccac tgccgtcaat
    32041 aagttctgtc aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct
    32101 gcaggcatct ttccattcaa actaacgttt cgctaccgtg aaagtgctac aaagataaag
    32161 atttggtttc aaaaagaaaa aatgtgtgac aaaaagtaaa aattaa
//
LOCUS       DADTFG010000043        31316 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.043, whole genome shotgun sequence.
ACCESSION   DADTFG010000043 DADTFG010000000
VERSION     DADTFG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.043"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(210..755)
                     /gene="orn"
                     /locus_tag="J1138_001826"
     CDS             complement(210..755)
                     /gene="orn"
                     /locus_tag="J1138_001826"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBA8453634.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            850..1902
                     /gene="rsgA"
                     /locus_tag="J1138_001827"
     CDS             850..1902
                     /gene="rsgA"
                     /locus_tag="J1138_001827"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBA8453635.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            1999..2967
                     /gene="psd"
                     /locus_tag="J1138_001828"
     CDS             1999..2967
                     /gene="psd"
                     /locus_tag="J1138_001828"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBA8453636.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            2989..6312
                     /gene="mscM"
                     /locus_tag="J1138_001829"
     CDS             2989..6312
                     /gene="mscM"
                     /locus_tag="J1138_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBA8453637.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6463..7965)
                     /gene="yjeM"
                     /locus_tag="J1138_001830"
     CDS             complement(6463..7965)
                     /gene="yjeM"
                     /locus_tag="J1138_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBA8453638.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNTMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8184..9161)
                     /gene="epmA"
                     /locus_tag="J1138_001831"
     CDS             complement(8184..9161)
                     /gene="epmA"
                     /locus_tag="J1138_001831"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBA8453639.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9486..11294
                     /gene="frdA"
                     /locus_tag="J1138_001832"
     CDS             9486..11294
                     /gene="frdA"
                     /locus_tag="J1138_001832"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBA8453640.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11287..12021
                     /locus_tag="J1138_001833"
     CDS             11287..12021
                     /locus_tag="J1138_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HBA8453641.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNARTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12032..12427
                     /gene="frdC"
                     /locus_tag="J1138_001834"
     CDS             12032..12427
                     /gene="frdC"
                     /locus_tag="J1138_001834"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBA8453642.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12438..12797
                     /gene="frdD"
                     /locus_tag="J1138_001835"
     CDS             12438..12797
                     /gene="frdD"
                     /locus_tag="J1138_001835"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBA8453643.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12860..13993
                     /gene="blaEC"
                     /locus_tag="J1138_001836"
     CDS             12860..13993
                     /gene="blaEC"
                     /locus_tag="J1138_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBA8453644.1"
                     /translation="MLKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPFDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            14082..14615
                     /locus_tag="J1138_001837"
     CDS             14082..14615
                     /locus_tag="J1138_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HBA8453645.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14612..14929)
                     /locus_tag="J1138_001838"
     CDS             complement(14612..14929)
                     /locus_tag="J1138_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HBA8453646.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15105..15251)
                     /gene="ecnB"
                     /locus_tag="J1138_001839"
     CDS             complement(15105..15251)
                     /gene="ecnB"
                     /locus_tag="J1138_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBA8453647.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15362..15487)
                     /gene="ecnA"
                     /locus_tag="J1138_001840"
     CDS             complement(15362..15487)
                     /gene="ecnA"
                     /locus_tag="J1138_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBA8453648.1"
                     /translation="MMKRLIVLVLLASTLFTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15539..16105)
                     /gene="efp"
                     /locus_tag="J1138_001841"
     CDS             complement(15539..16105)
                     /gene="efp"
                     /locus_tag="J1138_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBA8453649.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16147..17175
                     /gene="epmB"
                     /locus_tag="J1138_001842"
     CDS             16147..17175
                     /gene="epmB"
                     /locus_tag="J1138_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBA8453650.1"
                     /translation="MAHIVTLNPPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17410..18279
                     /locus_tag="J1138_001843"
     CDS             17410..18279
                     /locus_tag="J1138_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453651.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18329..18682)
                     /locus_tag="J1138_001844"
     CDS             complement(18329..18682)
                     /locus_tag="J1138_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBA8453652.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(18820..20466)
                     /gene="groL"
                     /locus_tag="J1138_001845"
     CDS             complement(18820..20466)
                     /gene="groL"
                     /locus_tag="J1138_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBA8453653.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(20510..20803)
                     /locus_tag="J1138_001846"
     CDS             complement(20510..20803)
                     /locus_tag="J1138_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBA8453654.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21079..22335
                     /gene="yjeH"
                     /locus_tag="J1138_001847"
     CDS             21079..22335
                     /gene="yjeH"
                     /locus_tag="J1138_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBA8453655.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSGQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(22351..22827)
                     /gene="fxsA"
                     /locus_tag="J1138_001848"
     CDS             complement(22351..22827)
                     /gene="fxsA"
                     /locus_tag="J1138_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBA8453656.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGSGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            23164..24600
                     /gene="aspA"
                     /locus_tag="J1138_001849"
     CDS             23164..24600
                     /gene="aspA"
                     /locus_tag="J1138_001849"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBA8453657.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            24718..26019
                     /locus_tag="J1138_001850"
     CDS             24718..26019
                     /locus_tag="J1138_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBA8453658.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26135..26473
                     /gene="cutA"
                     /locus_tag="J1138_001851"
     CDS             26135..26473
                     /gene="cutA"
                     /locus_tag="J1138_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBA8453659.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            26449..28146
                     /locus_tag="J1138_001852"
     CDS             26449..28146
                     /locus_tag="J1138_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBA8453660.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGETHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28183..28758
                     /locus_tag="J1138_001853"
     CDS             28183..28758
                     /locus_tag="J1138_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8453661.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            28865..28940
                     /locus_tag="J1138_001854"
     tRNA            28865..28940
                     /locus_tag="J1138_001854"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28898..28900,aa:Phe,seq:gaa)
     gene            29138..30400
                     /locus_tag="J1138_001855"
     CDS             29138..30400
                     /locus_tag="J1138_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA8453662.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGASMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDKARKLISEGIDPCEQKRTKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRKHRPALDLSRLPELLSRIDS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRRPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKFISPFEYAKINNPLK"
     gene            complement(30663..31282)
                     /locus_tag="J1138_001856"
                     /pseudo
     CDS             complement(30663..31282)
                     /locus_tag="J1138_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7772 a         8102 c         7852 g         7590 t
ORIGIN      
        1 tactacttaa ttttgctttc gtaattttcg catttctgcg tcgttacggg aggcgcatta
       61 tacgagaaat ctttccctct gcaacccccc taaaaaattt tttttgagtt tttatctcaa
      121 gtgctgaaat tatcagcaag acgttcaatt tagcgtcaaa atcacgccgc cgcaagtgga
      181 ttccggcgct tacgtgacca ggaaaaattt tacagcttga taaaatgctc gcggtagtaa
      241 gccaactccg ccaccgattc acggatatca tccatcgcct gatgcgtccc ctgcttggta
      301 aagccgtcaa gaatttccgg cttccagcgg cgcgccagct ctttcagggt gctaacatcg
      361 agatagcggt agtggaagta ggcttccagc tccggcatgt atttaaacag gaagcgacgg
      421 tcctgaccga tgctgttacc acaaatcggc gattttcccg caggcaccca ctgttttaag
      481 aattcgagcg ttgccagttc agcttcacga tcgcccatcg tgctcgcttt cacacgctct
      541 accagcccgc tggcggtatg ggtgcgcacg ttccagtcat ccatcagcgc cagctgttca
      601 tcggactgat gtactgcaat ggtcggccct tctgccagaa tattcaggtt ggcatcggtc
      661 accagcgtgg caatctcaat aatgcgatcg cgctcgggat ccagaccggt catctcaaga
      721 tcgatccaaa tcaggttgtt ttcattggca ctcatgctat tttccaccca ttcacagtga
      781 ctatattgat ccacaattgc gtgtatcata gatgttttgc ccatcagggg cgaccaggag
      841 tctgtacgat tgagtaaaaa taaactctcc aaaggccagc agcgccgcgt gaacgccaat
      901 caccagcgtc gtcttaaaac gtctaaggag aagcccgact acgacgacaa tctgtttggc
      961 gagcctgatg aaggtatcgt catcagccgc tttggtatgc acgctgatgt ggaatccgcc
     1021 gatggcgacg ttcaccgctg caatattcgc cgtaccatcc gttcgttggt gaccggcgac
     1081 cgcgtagtct ggcgtccggg taaaccagcg gcggaaggcg taaatgtcaa agggatcgtg
     1141 gaagcggtgc atgagcgcac ctcagtgttg acgcgcccgg atttttacga cggtgtgaaa
     1201 cctattgctg ccaacatcga ccagattgtc attgtctccg ccattttgcc ggagctgtcg
     1261 ctcaatatta tcgaccgtta cctggtggcc tgcgaaacct tgcagattga gccgattatt
     1321 gtgctcaaca agatagacct gctggatgac gaaggcatgg agttcgtcaa cgagcagatg
     1381 gatatctacc gcaatatcgg ttatcgcgta ttgatggttt ccagtcatac tcaggatggg
     1441 ctgaaaccgc tggaagaggc gttgaccggg cgcatcagca tttttgccgg gcagtctggc
     1501 gttggcaaat ccagcctgct gaacgcgttg ctggggctgc aaaaagagat cctgaccaac
     1561 gatgtctctg acaactcggg gcttggtcag cacaccacga ccgccgcacg actgtatcac
     1621 ttcccgcacg gcggtgatgt gattgattcc ccaggtgtgc gtgagtttgg tctctggcac
     1681 ctggagccgg aacaaatcac tcagggcttt gtcgaattcc atgactattt aggtctgtgt
     1741 aaatatcgcg attgcaaaca cgataccgat ccgggctgcg ctatccggga agcggttgag
     1801 gaaggaaaaa tcgcggaaac ccgtttcgaa aactatcacc gtattctgga aagcatggcg
     1861 caggtaaaaa cgcgtaaaaa cttttctgat acggatgact gacaattaag ctaaccgtcg
     1921 ttagaatcgt cccctttttt tcaggatccg gcatgtaggc cggatcagga acgacaaaac
     1981 aatggcctgg aggctacctt gttaaattca tttaaacttt cgctacagta cattctgccg
     2041 aaactatggc ttactcgcct ggcgggttgg ggcgcaagca agcgggcagg atggctgaca
     2101 aaactggtta tcgatctgtt cgttaaatac tacaaggtcg acatgaaaga ggcgcaaaag
     2161 ccggacaccg ccagctaccg cacctttaac gaattctttg tccgtccgct gcgtgacgaa
     2221 gtacgcccaa tcgataccga tccgaatgta ctggttatgc ctgccgatgg cgttatcagc
     2281 cagttgggta aaatcgaaga agataaaatc ctgcaagcca aaggccacaa ctacagcctc
     2341 gaagccctgc tggcaggcaa ctatctgatg gcggatctct tccgtaacgg tacgtttgtg
     2401 accacttacc tctccccacg tgactaccac cgcgtacaca tgccgtgtaa cggtattctg
     2461 cgtgaaatga tctacgtgcc gggcgatctc ttctccgtta accatctcac ggctcagaac
     2521 gtgccgaatc tgtttgcccg taacgaacgc gtgatctgcc tgttcgatac cgaatttggc
     2581 ccgatggcgc agattctggt cggtgcgacg attgtaggca gcattgagac ggtctgggcg
     2641 ggcaccatta cgccgccgcg cgaaggcatc atcaagcgct ggacctggcc tgccggggaa
     2701 aacgacggtt ctgtggcatt gctgaaaggc caggaaatgg gtcgctttaa acttggctct
     2761 accgttatca acctgtttgc accaggtaaa gtgaacctgg ttgagcaact ggaaagcctg
     2821 tctgtcacga aaattggcca gccgctggca gtatctaccg aaacctttgt tacgccagac
     2881 gctgaaccag ccccgcttcc tgctgaagag atcgaagcag aacacgacgc cagcccattg
     2941 gttgacgaca aaaaagacca ggtctaatcc atcaaaggaa acgctgacgt gcgcctgatt
     3001 atcacttttc tgatggcctg gtgcctcagt tggggggcgt acgccgcgac ggcccccgat
     3061 agcaaacaaa tcacccagga actggagcag gcaaaagcgg cgaaacccgc gcagccggaa
     3121 gtcgtagagg cgctccagtc tgccttaaat gcgcttgagg aacgaaaagg ttcccttgag
     3181 cgcatcaagc aatatcaaga agtcattgat aattatccga aactttccgc cactctgcgc
     3241 gcgcagttaa acaacatgcg tgacgagcca cgcagcgtgt cgccgggaat gtctaacgat
     3301 gcgctgaatc aggaaattct ccagatcagc agtcagttgc tggataaaag ccgtcaggcc
     3361 cagcaagagc aggagcgcgc ccgcgagatt gccgattcgc tgaatcaact gccgcaacag
     3421 caaaccgatg cccgccgtca gttgaatgaa atcgagcgcc gcctggggac gcttaccggc
     3481 aataccccgc tcaatcaggc acaaaatttc gcgttgcagt ctgactccgc acgtctgaaa
     3541 gcgctcgttg atgaactgga gctggcgcag ctatctgcca ataaccgcca ggaattagcg
     3601 cgcttgcgct ctgagctggc ggaaaaagag agccagcagc tggatgcata tttgcaagca
     3661 ctacgcaacc agttgaatag tcaacgccaa ctggaagcgg aacgggcgct ggaaagtacc
     3721 gagcaactgg cggaaagtag cgccgatttg ccaaaagata tcgtcgccca gtttaaaatt
     3781 aaccgcgaac tatcggcggc gctgaatcaa caggcgcagc ggatggatct cgttgcctcg
     3841 caacagcgtc aggctgccag ccagacgtta caggtccggc aggcgttgaa tacgctgcgt
     3901 gaacagtcgc aatggctggg atcatctaac ctgctcggcg aagcgttgcg ggcgcaggtg
     3961 gcacggctac cggaaatgcc aaaaccacaa cagcttgata ccgaaatggc gcagttacgt
     4021 gtgcaacggt tacgttatga ggatctgctt aataaacagc cgctgctacg gcaaatccat
     4081 caggccgacg gtcagccact gaccgctgag caaaaccgta ttctggaagc acagctacgc
     4141 actcagcgtg agttgctgaa ctcattgttg cagggtggcg acacgctact gctggagctg
     4201 accaagctga aagtctccaa cgggcaactg gaggatgcgc tgaaagaggt gaacgaagcg
     4261 acgcaccgct atctgttctg gacctctgac gtgcgcccga tgaccatcgc ctggccgctg
     4321 gaaatcgccc aggatctgcg tcgtctcatt tcgctggaca ccttcagtca gttgggcaaa
     4381 gccagtgtga tgatgctgac cagtaaggag acgattttgc cgctgtttgg cgcgttgatt
     4441 ctggtcggtt gcagtattta ctcgcgccgc tatttcaccc gttttcttga acgttcggcg
     4501 gcgaaagtcg gcaaagtgac tcaggatcac ttctggctga cgttacgcac tcttttctgg
     4561 tcgattctcg tcgcgtcacc gctgcctgtg ctgtggatga cactgggtta cggcttgcgc
     4621 gaggcgtggc cttatccgct ggcggtcgcg attggtgatg gcgtaacagc caccgtgccg
     4681 ctgctgtggg tagtgatgat ttgcgccacc tttgctcgcc cgaacggctt gtttatcgct
     4741 cattttggct ggccgcgcga acgagtttcc cgtgggatgc gttactacct gatgagcatc
     4801 gggcttattg tgccgctgat tatggcgctg atgatgttcg ataacctcga cgaccgtgaa
     4861 ttctccggtt cgctgggacg gctttgcttt atcctcattt gcggtgcgct ggcggtggtc
     4921 accctcagcc tgaaaaaggc cgggatcccg ctgtatctca acaaagaggg cagcggcgac
     4981 aacattacca accatatgct gtggaacatg atgattggcg caccgctggt tgccattctg
     5041 gcgtcggcgg tgggttatct ggcaacggcg caggcgctgt tagcgaggct tgaaacctcg
     5101 gttgccatct ggtttttgct actggtggtt tatcacgtta tccgccgctg gatgctgatc
     5161 cagcgccgca ggctggcgtt tgaccgggcg aagcatcgcc gggcagagat gttagcgcag
     5221 cgcgcgcgtg gcgaagagga agcgcatcat cacagtagcc cggaaggagc aattgaagtc
     5281 gatgaaagcg aagtcgatct cgatgccatc agtgcgcaat ccttgcggct ggtgcgctca
     5341 attttgatgt tgatcgccct gctttcggtc attgtgctgt ggtcagaaat ccattccgct
     5401 ttcggcttcc tcgaaaatat ttcgctgtgg gatgtcactt ccacggtaca gggcgtagaa
     5461 agcctggagc cgattaccct cggtgcggta ctgattgcca ttctggtgtt tatcatcacc
     5521 acgcagctgg tgcgcaacct gcccgcgctg ctggaactgg cgattctgca gcacctggat
     5581 ttaacgccgg gtacgggcta cgccatcacc accatcacca aatatctgct gatgctgatt
     5641 ggcgggctgg tcggcttctc gatgattggt attgagtggt cgaaattgca gtggctggtc
     5701 gccgcgctcg gtgttggtct cggttttggt ttgcaggaaa ttttcgccaa ctttatctct
     5761 ggcctgatca tcctgttcga aaaaccgatt cgcattggcg atacggtaac gatccgcgat
     5821 cttaccggta gtgtgacgaa aattaacacc cgcgccacca ccatcagcga ctgggaccgc
     5881 aaagaaatta tcgtgccgaa caaggcgttt attaccgagc agtttatcaa ctggtcgctc
     5941 tctgactcgg tcacgcgtgt ggtgttgaca attccggccc ctgccgatgc caacagtgaa
     6001 gaagtgacgg aaatcctgct caccgcagcg cgtcgctgct cgctggtgat cgacaacccg
     6061 gcgccggaag tcttcctggt ggatctgcaa caggggattc aaattttcga gctgcgtatt
     6121 tacgccgctg agatgggtca ccgtatgccg ctacgccatg agatccacca actaattctg
     6181 gcgggcttcc acgcccacgg tatcgatatg ccattcccgc ccttccagat gcgtctggaa
     6241 agcctgaatg gtaaacaaac ggggagaaca ttaacttctg cgggcaaagg tcgtcaggcg
     6301 ggaagtttgt aatgcatgaa gctgccaaat gatgtcaacg tggctcattt agcagcttaa
     6361 catcatcttc agttatcact ggccccggag agtatcatcc atacgcatta cttcctgaca
     6421 actcatcgcc ggagagacga tgagttgcag gatttcatat gatcagtcga gagctaactc
     6481 aggtttgttc gccatgcgac tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa
     6541 gaagacaggg ccgccaatca tccacaatgt gctgtcccag tctccagctt caaccacagg
     6601 ttgaatgatg gtgaagacgt tcgcaaatgt caccaccagt acgacaacca ctgttgcaat
     6661 cattgccgac atatgcgttt tgaaaatcac aaacggtctg tcgagatcct gacgtgcttt
     6721 aaaaaatggg aaagccagcg cgaggaacag gtaaggaagc gtcatagaca cgttcgccat
     6781 cagcgtcagc ttgttaaaga acgccgatgc ggtaccgcca ccaaacgaaa ccagcagaat
     6841 gaagatagta accaacccgc actgcatcca catggcaata gacggcatcc ccatcgtatt
     6901 caggcgcgtc atcggttccg gccacaatgc tttcggcgtc ccctggatga tggctttcag
     6961 cggtgaatag caaagtgtaa agaacgcacc ggtataagcg aggaacatcg acagtccggt
     7021 aatacgcgca aaccatacgc ccagcgacaa tgacgcttca ggtgacaaat gcagtgcgtt
     7081 acccagcgtc acccctaggc tcttcatcag cacataggta atattgccga ggttaacgga
     7141 accattactt aatacctgct gccagtttgt gctgacgccc cataaaaata ttgccagcga
     7201 ataaccgatt gaaataacaa tagcggcaaa aacaatacct ttggcaaagt tcttttctgg
     7261 attttccgtt ttatcgacca gaccaccaac cgcttcaatt ccgccatagg caaaaatggc
     7321 aaataccaca aacgatagca ttgccagacc ggactgataa cctgggttcg gtgatgcaag
     7381 gaaattaata tcctgcgcga aatgcccacc atttaataac aaaatagtaa tgcttactaa
     7441 cagcaatact aaattcagac acattactgc aataccgccc accgcagtaa tgcgggcaat
     7501 tttattaatc ccttttgaag caacgacggt gaccagaatc atccatgcca ctgccagcag
     7561 accaaccacc tgcgtaggct ccagtccggc aatatgccag tgctgggtca tgtcgctacc
     7621 gtagaggaat gttgagaacg gtacccaaac tttcgcggag gtactcacca tccagatgat
     7681 ataagaggaa aaccacataa acgtaccaat gaaggcaaaa cgtggtccga cactattatt
     7741 catccaggaa tagataccgc cttcttcttt gcgataagca gctcccattt cagccatcat
     7801 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
     7861 acccattaag taataagccg atgggctatt ggcaaatcca aatacagaag taaagatcat
     7921 caatatgagt cctatcagac tcatcttttt tatcgagtga ggcatgaaac catccttcat
     7981 ttcagcgcgt gtataaaacg cgcccactga actgcactgc ccaacattga gcgggcagcg
     8041 taaaaaccaa tacagaattg atacctggat gatatcaaat atccagtctt aaattgtggc
     8101 ctttagcgga aaaatgaaaa catatgctac agaatggcgc ttatcacgcc attcttcgct
     8161 gttaattcag tgatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
     8221 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
     8281 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
     8341 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
     8401 tccgtcaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
     8461 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
     8521 tggtacacaa aggtcggttt ttctttgcca atatttggct ctacgccaaa ggtaaacagc
     8581 aattgtagaa gcgtgtcgcg gtcttcttca gtatcggcaa cgttgctcaa atccagtttc
     8641 gctgccactt cccgcagttg cgttttgtcg gcagagagtg ggtcaatttc cagataacgc
     8701 aagaaagctt gttgataaga aaggctttct gctgccggac agtccagcac ctgttgcaag
     8761 agatcgtcca cctcgttcat caatcggtac atatcatagt gcggtcgata ccactcaagc
     8821 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     8881 tggaataccg gcccacaacc ggcaaccagc aggcgcttca tatggtattc cgggctggtc
     8941 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     9001 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     9061 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     9121 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     9181 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaataaa
     9241 acaacggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     9301 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     9361 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     9421 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     9481 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     9541 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     9601 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     9661 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     9721 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     9781 tgtgggggtg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     9841 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     9901 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     9961 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaatgaac atgatggaag
    10021 gcacgctggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
    10081 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
    10141 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctaccag
    10201 gttccggtat cctgatgacc gaaggctgcc gcggtgaagg tggtattctg gtcaacaaaa
    10261 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
    10321 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
    10381 ggcgtaaagg caacaccatc tccacgccac gtggcgatgt ggtttacctc gacctgcgtc
    10441 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
    10501 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgaccgca cactacacca
    10561 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
    10621 gtgaatgttc ctctgttggt ctgcacggtg cgaaccgtct gggctccaac tcgctggcgg
    10681 aactggtggt cttcggtcgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
    10741 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
    10801 atctggttaa ccaggatggc ggcgaaaact gggctaagat ccgcgacgaa atgggcatgg
    10861 ccatggaaga aggctgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
    10921 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacact tccagcgtgt
    10981 tcaacaccga cctgctctac accattgagc tgggccacgg tctgaacgtt gctgaatgta
    11041 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc gcaccagcgt ctggacgaag
    11101 gttgcaccga gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg
    11161 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
    11221 gtgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aagaaggaga
    11281 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
    11341 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
    11401 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagctaccg ctggtcctgc
    11461 cgtatggcga tttgtggctc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
    11521 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
    11581 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
    11641 ccgtacatca tcggcaacgc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
    11701 cagatggcga agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
    11761 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
    11821 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
    11881 cagaacggcg tatggagctg tactttcgtg ggctactgct ccgaagtctg cccgaaacac
    11941 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
    12001 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
    12061 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
    12121 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
    12181 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
    12241 gatcattaac ctgatcactc tggcggcagc cctgctgcac accaaaacct ggtttgagct
    12301 ggcaccaaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
    12361 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
    12421 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
    12481 ggggcctctt cggggccggt ggtatgtgga gcgccatcat tgcgccggtg atgatcctgc
    12541 tggttggtat tctgctgccg ctagggctgt ttccgggtga tgcactgagc tacgagcgcg
    12601 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
    12661 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa attcacgtac
    12721 ctgcgggtaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
    12781 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
    12841 tgtatggaaa ccagacctta tgctcaaaac gacgctctgc accttattaa ttaccgcctc
    12901 ttgctccaca tttgccgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
    12961 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
    13021 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
    13081 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
    13141 tgctcgaggg gaaatcaagt taagcgatcc cacaacaaaa tactggcctg aacttaccgc
    13201 taaacagtgg aatgggatca cactattaca tctcgcaacc tacaccgctg gcggcctgcc
    13261 attgcaggtg ccggatgagg tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
    13321 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
    13381 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatga aaactcgtgt
    13441 cttccagcca ctcaaactca atcatacgtg gattaatgta ccgtccgcag aagaaaagaa
    13501 ctacgcctgg ggatatcgcg aaggtaaggc agtacatgtt tcgccagggg cgttagatgc
    13561 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
    13621 aaaacccttt gatatcaatg agaaaatact tcaacaaggg atacagctgg cacaatctcg
    13681 ctactggcaa accggcgata tgtatcaggg tctgggctgg gaaatgctgg actggccggt
    13741 aaatcctgac atcatcatta acggcagtga caataaaatt gcactggcag cacgccccgt
    13801 aaaaccgatt acgcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
    13861 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
    13921 gctggctaac aaaaactatc ccaatccagc gagagttacc gccgcctggc agattctcaa
    13981 cactctacag taaaatttca tcgggtccga attttcggac cttttctccg cttttccttg
    14041 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
    14101 ttgccgcagc gacagctgca tttctggtcg ttgcctgcag ttcccctacg ccgccgcgtg
    14161 gcgtgaccgt agtgaataat ttcgacgcta aacgttatct cggtacctgg tatgagattg
    14221 cccgttttga tcaccgcttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
    14281 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga gaaatgtggc
    14341 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
    14401 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
    14461 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
    14521 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
    14581 gtaagtttat ttgggtacag cagcccggta gttaatgagt gctgagtttc agaccaataa
    14641 tccccaatac gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcaccgagca
    14701 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
    14761 tccctactgg taacgatttc atcgcccagg caagtagcgc cagactgaca atcatcgccg
    14821 tcacggtaat aacacttggc gtcaaacgac taaagccgtg agtatatttc aggccaacgg
    14881 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
    14941 gaaaattggg gccgtccccg gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg
    15001 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattacctat gtaactatcg
    15061 cagaaaaaac aaacggcacg acacagctgt cgtaccgtta ttgcttattg ctgcgctttc
    15121 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
    15181 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
    15241 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
    15301 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
    15361 attagctggc agcgcgggag atggagttgc cgaggtgttt gatatcttcg ccgaaaccgc
    15421 gagcggtgtt acagcccgta aacagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
    15481 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
    15541 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
    15601 gtacaaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
    15661 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
    15721 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
    15781 ccagcaacca tttagcgttg tcgccaattg cttttgcgtc agcagacagc tgctcgaaag
    15841 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
    15901 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
    15961 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
    16021 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
    16081 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
    16141 cacaaaatgg cgcatattgt aaccctaaat cccccatcca gagaagattg gttaacgcaa
    16201 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
    16261 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
    16321 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
    16381 tcgcaagatg agtttgtcgt cgcgcccgga ttctccaccg acccgctgga agaacagcat
    16441 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
    16501 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
    16561 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
    16621 gagatgattt tctccggcgg cgatccgctg atggcgaaag atcacgagct ggactggttg
    16681 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
    16741 attgtgatcc cggcacgcat cacagatgcg ctggttgaac gcttttctca ttctacgctg
    16801 caaatcttgc tggtgaatca catcaaccat gccaatgagg tagatgaaac attccgtcag
    16861 gcgatggcta agttgcgccg ggtgggcgtt actttgctga accagagcgt tctgttacgt
    16921 ggtgtgaacg ataacgcgca aacgctggca aacctgagta atgcgttgtt cgatgcgggc
    16981 gtgatgccgt attacctgca tgtgctcgat aaagtacagg gcgcggcgca ttttatggtg
    17041 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
    17101 gtgcctaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
    17161 ctacgccagc agtaactgat taaacaggga agtttaatct ttgcccgttg cataaccttt
    17221 aattattcat atacattgct gttgttactc tttatataac caacaccaat aattaacctt
    17281 tcttttctgt ttatatagag tgagcacggc tatttatgtt attttaacaa ctattactac
    17341 caagcccatt tatctggctt ataaactaaa attaattatt taattgagaa atggttaggg
    17401 agaacctaca tggccataag catcaagggt gttaataccg gtgttattcg caagagtaat
    17461 aatttcattg cactggcgct gaaaattaaa gaacctcgca ataaagaatc gttgttcttt
    17521 atgtctgcca tggaattgcg cgaccttctg attgcgctgg aaagtcgtct gcatcaaaag
    17581 cataaactgg atgctgctgc acgccttcag tatgaacaag ctcgcgataa agtcattaag
    17641 aaaatggcag aaaatatccc agaaattctg gttgatgagc ttaaaaatgc cgatattaat
    17701 cgtcgggtta acacgctgga acttaccgat gatcagggcg aaaatcttac cttcgtatta
    17761 acgctacatg acggtagcaa atgtgagtta gtcgtcaatg agttgcagat tgaaatgctg
    17821 gcgcgagcaa tcattcatgc aattaataat gcggaaatgc gtgaactggc attacgtatt
    17881 acctcactgt tagatttcct gccgttatac gacgttgact gccaggaaaa tggcaatctg
    17941 gaatatgaca cctattccca accggaatgg aaacataact tgttcaacca ttatctggcg
    18001 gtgctttacc gctttaaaga tgaaagtggc aatgaacaat ttagcggcgc agtggtgaaa
    18061 acgcgtgaag caacgccggg taaagaagtg gaggcgatca ctcgccgaat gcttgatttc
    18121 agcccccgac tgaaaaaact cgcgggtgtg ccatgtcagg tctatgttcg caccgtagcg
    18181 gcaaataacg cccaaccgct aacccaggat caatgcttac gcgcgctgca tcacttacgt
    18241 gttcaatcca ccagcaaaac tgcaccacag gcgaaataat ctcatctgtt ttactacgag
    18301 gctggaagta tccagcctct ggatcacatc agttcgggca cttataaacc tgaccgataa
    18361 tctggctatc cgtcgggaca aaactggaca acattccctg cgacgggctg ctgatgccat
    18421 aaatcacgtt accgcccatt gcagccgcct ggttgcgcag atcgtttgct gcgccgcgca
    18481 tagaaccgcc ttcttctccg tgttgcccgg aaagccagtt gctttgctta cctgtcgcag
    18541 taccaatcag ctggcactct gcacctggtt gctcgtccac aatgcgtaca ctctgaccgg
    18601 cagcactcaa ttcgttgctg gagctacaac ccgccatcag tagcgcggca ccgacaatcc
    18661 ctgctaagta ttttacgtgc atgttattcc ccataatcaa tgagctggac gccactcgcg
    18721 tcgtccgtgt ctgaatctta tactagaaag atgaccaaaa gaaaaacccc cagacatttc
    18781 tgcccggggg tttgtttatt tctgcgaggt gcagggcaat tacatcatgc cgcccatgcc
    18841 acccatgccg cccataccgc cagcagcgcc taagtcagct gcatcgtttt tcggcaggtc
    18901 ggtaaccatg cactcggtgg tgatcatcag gccagccaca gaagccgcgt actgcagagc
    18961 agaacgggtt actttggttg gatccaggat acccatatcg atcatgttgc cgtattcttc
    19021 agttgctgcg ttgtaaccgt agttgccgtc gccgccttta acggtgttag caacaacaga
    19081 cggttcttcg ccgcagttca ggacgatctg acgcagcgga gcttccattg cacgcagtgc
    19141 aactttgata cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca gtttagacgc
    19201 tacgcggatc agcgcaacac caccaccagc aaccacgcct tcttctaccg cagcacgggt
    19261 cgcgtgcagg gcgtcttcaa cgcgtgcttt tttctctttc atttcaactt cggtagcagc
    19321 accaactttg ataactgcaa cgccgcctgc cagtttcgct acgcgctcct gcagtttttc
    19381 acggtcgtag tcagaagttg cttcttcaat ctgctgacgg atctgagcaa cacggccctg
    19441 gattgcagct tcttcaccca cgccatcgat gatggtggtg gtgtctttgt tgatcacaac
    19501 acgtttagcc tgacccaggt cttccagggt tgctttttcc agctccatac cgatctcttc
    19561 agagattacg gtaccgccag tcagggttgc gatatcctgc agcatagctt tacgacgatc
    19621 gccgaagccc ggagctttaa ccgcagcaac tttcacgatg ccacgcatgg tgttaacaac
    19681 cagagttgcc agtgcttcgc cttcaacatc ttcagcgatg atcagcagcg gtttacctgc
    19741 tttggcaaca gcttccagaa ccggcagcat ttcgcggatg ttggagattt tcttgtcagc
    19801 caacaggatg aacgggcttt ccagttctac tgcgccagtt tccggcttgt tgatgaagta
    19861 aggagacagg tagccacggt cgaactgcat accttcaacc acgtccagtt cgtcctgcag
    19921 accggtaccg tcttcaacgg tgataacgcc ttctttaccg actttgtcca tcgcttcagc
    19981 gatcagttta cctacggttt cgtcggagtt agcagagatg gtaccaacct gagcaatcgc
    20041 tttagagtca gagcacggta cggacagcgc tttcagttct tcaactgcag cggtaactgc
    20101 tttgtcgata ccacgtttca ggtccatcgg gttcatgccc gcagcaacag ctttcagacc
    20161 ctcagtgatg atagcctgag ccagtacggt tgcagtggtg gtaccgtcgc ctgcagcgtc
    20221 gttcgcttta gaggcaactt ctttcaccat ctgcgcaccc atgttttcga acttgtcttc
    20281 cagttcgatt tcacgagcaa cggaaacacc atctttggtg atggtcggtg caccgaaaga
    20341 tttatccaga actacgttac ggcctttcgg accgagggta actttcactg catctgccag
    20401 tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta cgtctttagc
    20461 tgccattatc tttattcctt aaattcgtat gttcagtgtc gtgcgcggat tacgcttcaa
    20521 caattgccag aatgtcgctt tcggacatga tcaacacttc ttcattgtcg atcttctcag
    20581 atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc aactttcaca tccagcggct
    20641 tcacttcgcc attttcaagg atacggccat tgccgacagc cagcacttcg ccgcgggtgg
    20701 atttagccgc tgcagagccg gtcagaacga tgccgccagc agatttagtt tcaacttctt
    20761 tacgcttgac gatcacgcga tcatgcaatg gacgaatatt cattgataac tctcctttga
    20821 gaaagtccgt atctgttatg ggtgacgccg gagcttacgt ggtttcccgg ctggtgacca
    20881 gagaaatggg gatgaggctt cgccccttca agggggaaaa agaaaaaaaa ttctgatcag
    20941 cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca tcgctatttc ttcatcaccg
    21001 tcataagcga atctgattgt gctaccatcg aaaatctacg cagttgccca aaatttgggc
    21061 gcaatcggac atcaacccat gagtggactc aaacaagaac tggggctggc ccagggcatc
    21121 ggcctgctat cgacgtcatt attaggcact ggcgtgtttg ccgttcctgc gttagctgcg
    21181 ctagtagcag gcaataacag cctgtgggcg tggcccgttt tgattatctt agtgttcccg
    21241 attgcgattg tgtttgcgat tctgggtcgc cactatccca gcgcaggcgg cgtcgcacac
    21301 ttcgtcggta tggcgtttgg ttcgcggctt gagcgagtca ccggctggtt gtttttatcg
    21361 gtcattcccg tgggtttgcc tgccgcgcta caaattgctg ccggattcgg ccaggcaatg
    21421 tttggctggc atagcgggca actgttgttg gcagaactcg gtacgctggc gctggtgtgg
    21481 tatatcggta ctcgaggtgc cagttccagt gctaatctac aaacagttat tgccgggctt
    21541 atcgtcgcac tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc gaatatcccc
    21601 ttccctgcgc caggaaatat cgaacttacc gggttattcg ctgcgttatc agtgatgttc
    21661 tggtgttttg tcggtctgga agcatttgcc catcttgcct cggaatttaa aaatccagag
    21721 cgtgattttc ctcgtgcttt gatgattggc ctgctgctgg caggattagt ctattggggc
    21781 tgtacggtag tcgtcttaca cttcgacgcc tatggtgaac aaatggcggc ggcagcatcg
    21841 cttcccaaaa ttgtagtgca gttgttcggt gtaggagcgt tatggattgc ctgcgtaatt
    21901 ggctatctgg cctgctttgc cagtctcaac atttatatac agagcttcgc ccgcctggtc
    21961 tggtcgcagg cgcaacataa tcctgaccac tacctggcac gcctctcttc tcgccatatt
    22021 ccgaataatg ccctcaatgc ggtgctcggc tgctgcgtgg tgagcacgtt ggtgattcat
    22081 gctttagaga tcaatctgga cgctcttatt atttatgcca atggcatctt tattatgatt
    22141 tatctgttat gcatgctggc aggctgtaaa ttattgcaag gacgttatcg actactggca
    22201 gtggttggcg ggctattatg cgttctgtta ctggcaatgg tcggctggaa aagtctctac
    22261 gcgctgatca tgctggcggg gttatggctg tttctgccaa aacgaaaaac gccggaaaat
    22321 ggcataacca cataatccgg cgtttcgaca ttaatcctgg cgatcgtctt tatgatcaag
    22381 gcggtcgcgg tcatcatcct ttcgctggta ctcaccatca aaagtattac cgctaccggt
    22441 cccggcgcta aaaccgccgc ctggcatacg ggaaaaacgc agatgaggca tcaacttcac
    22501 tgtcagatgc ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt cggtgaaaaa
    22561 gcccggtaat aaaagcagca aaccggcaat gatcagcgaa acacttttaa tcatctccgc
    22621 cgctgggttt tcacccgccg ccattttttg ctgcatcagc acgaaattct taaagccctg
    22681 gttgcgtacc agtgacatac cgataactga cgtgaatata accagcacga gggtcagcaa
    22741 tacccccaat acatgagcaa cctgaataaa gattgaaatc tcaatataga cataaaggaa
    22801 aatggcaata aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc ggttaaaccc
    22861 ggctacctga atgggttgcg aatcgcgttt agcttatatt gtggtcatta gcaaaatttc
    22921 aagatgtttg cgcaactatt tttggtagta atcccaaagc ggtgatccat ttcacaaatt
    22981 aataattaag gggtaaaaac cgacacttaa agtgatccag gttacggtag aaatcctcaa
    23041 gcagcatatg atctcgggta ttcggtcgat gcaggggata atcgtcggcc gaaaaacatt
    23101 cgaaaccaca tatattctgt gtgtttaaag caaatcattg gcagcttgaa aaagaaggtt
    23161 cacatgtcaa acaacattcg tatcgaagaa gatctgttgg gtaccaggga agttccagct
    23221 gatgcctact atggtgttca cactctgaga gcgattgaaa acttctatat cagcaacaac
    23281 aaaatcagtg atattcctga atttgttcgc ggtatggtaa tggttaaaaa agccgcagct
    23341 atggcaaaca aagagctgca aaccattcct aaaagtgtag cgaatgccat cattgccgca
    23401 tgtgatgaag tcctgaacaa cggaaaatgc atggatcagt tcccggtaga cgtctaccag
    23461 ggcggcgcag gtacttccgt aaacatgaac accaacgaag tgctggccaa tatcggtctg
    23521 gaactgatgg gtcaccagaa aggtgaatat cagtacctga acccgaacga ccatgttaac
    23581 aaatgtcagt ccactaacga cgcctacccg accggtttcc gtatcgcagt ttactcttcc
    23641 ctgattaaac tggtagatgc gattaaccaa ctgcgtgaag gctttgaacg taaagctgtc
    23701 gaattccagg acatcctgaa aatgggtcgt acccagctgc aggacgcagt accgatgacc
    23761 ctcggtcagg aattccgcgc cttcagcatc ctgctgaaag aagaagtgaa aaatatccaa
    23821 cgtaccgctg aactgctgct ggaagttaac cttggcgcaa cagcaatcgg tactggtctg
    23881 aacacgccga aagagtactc tccgctggca gtgaaaaaac tggctgaagt cactggcttc
    23941 ccatgcgtac cggctgaaga cctgatcgaa gcgacctccg actgcggcgc ttatgttatg
    24001 gttcacggcg cgctgaaacg cctggctgtg aagatgtcca aaatctgtaa cgacctgcgc
    24061 ttgctctctt ctggtccacg tgccggcctg aacgagatca acctgccgga actgcaggca
    24121 ggctcttcca tcatgccagc taaagtaaac ccggttgttc cggaagtggt taaccaggta
    24181 tgcttcaaag tcatcggtaa cgacaccact gttaccatgg cagcagaagc aggtcagctg
    24241 cagttgaacg ttatggagcc agtcattggc caggccatgt tcgaatccgt tcacattctg
    24301 accaacgctt gctacaacct gctggaaaaa tgcattaacg gcatcactgc taacaaagaa
    24361 gtgtgcgaag gttacgttta caactctatc ggtatcgtta cttacctgaa cccgttcatc
    24421 ggtcaccaca acggtgacat cgtgggtaaa atctgtgccg aaaccggtaa gagtgtacgt
    24481 gaagtcgttc tggaacgcgg tctgttgact gaagcggaac ttgacgatat tttctccgta
    24541 cagaatctga tgcacccggc ttacaaagca aaacgctata ctgatgaaag cgaacagtaa
    24601 tcgtacaggg tagtacaaat aaagaaggca cgtcagatgg cgtgcctttt ttcttgtgag
    24661 cagtaactta aaaataacaa tttaatatca acttgttaaa aaacaaggaa ggctaatatg
    24721 ctagttgtag aactcatcat agttttgctg gcgatcttct tgggcgccag attgggggga
    24781 ataggtattg gttttgcagg cggattgggg gtgctggttc ttgccgctat tggcgttaaa
    24841 cctggtaaca tcccgttcga tgtcatttcc attatcatgg cggttatcgc cgctatttct
    24901 gccatgcagg ttgctggcgg tctggactat ctggttcatc agacagaaaa gctgctgcgc
    24961 cgtaacccga aatacatcac gatcctcgca ccgatcgtga cctatttcct gactatcttt
    25021 gctggtactg gcaacatctc tctggcgaca ctgccagtta tcgctgaagt tgcgaaggaa
    25081 caaggcgtta aaccttgccg tccgctgtct actgcagtgg tatccgcgca gattgcgatc
    25141 accgcatcgc caatctcagc ggcagtggtt tacatgtctt ccgtgatgga aggtcatggc
    25201 atcagctacc tccatctgct ctccgtggtc atcccgtcca ccctgctggc ggttctggtg
    25261 atgtccttcc tggtcactat gctgttcaac tccaaactct ctgacgatcc gatttatcgc
    25321 aagcgtctgg aagagggcct ggttgaactg cgcggtgaaa agcagattga aatcaaatcc
    25381 ggtgcaaaaa cgtccgtctg gctgttcctg ctgggcgtag ttggcgtggt tatctatgca
    25441 atcatcaaca gcccaagcat gggtctggtt gaaaaaccac tgatgaacac caccaacgca
    25501 atcctgatca tcatgctcag cgttgcaact ctgaccaccg ttatctgtaa agtcgatacc
    25561 gacaacattc tcaactccag caccttcaaa gcaggtatga gcgcctgtat ttgtatcctg
    25621 ggtgttgcgt ggctgggcga tactttcgtt tccaacaaca tcgactggat caaagatacc
    25681 gctggtgaag tgattcaggg tcatccgtgg ctgctggccg tcatcttctt ctttgcttct
    25741 gctctgctgt actctcaggc tgcaaccgca aaagcactga tgccgatggc tctggcactg
    25801 aacgtttctc cgctgaccgc tgttgcttct ttcgctgcgg tgtctggtct gttcattcta
    25861 ccgacctacc cgacgctggt tgctgcggta cagatggatg acacgggtac tacccgtatc
    25921 ggtaaattcg tcttcaacca tccgttcttc atcccgggta ctctgggtgt tgccctggcc
    25981 gtttgcttcg gcttcgtgct gggtagcttc atgctgtaat gacccatcgc ggggcgttca
    26041 cgccccgctt tctttcccgc cgactaacat cctttccccg tccgttgtat agtgacctct
    26101 ctcttgcggt tccatctgtt cttgcgaggt gtttatgctt gatgaaaaaa gttcgaatac
    26161 cacgtctgtc gtggtgctat gtacggcacc ggatgaagcg acagcccagg atttagccgc
    26221 caaagtgctg gcggaaaaac tggcggcctg cgcgaccttg atccccggcg ctacctctct
    26281 ctattactgg gaaggtaagc tggagcaaga atacgaagtg cagatgattt taaaaactac
    26341 cgtatctcac cagcaggcac tgctggaatg cctgaagtct catcatccat atcaaacccc
    26401 ggaacttctg gttttacctg ttacacacgg agacacagat tacctctcat ggctcaacgc
    26461 atctttacgc tgatcctgct actttgcagc acttccgttt ttgccggatt attcgacgcg
    26521 ccgggacgtt cacaatttgt ccccgcggat caagcctttg cttttgattt tcagcaaaat
    26581 caacatgatc tgaatctgac ctggcagatc aaagacggtt actacctcta ccgtaaacag
    26641 atccgcatta cgccggaaca cgcgaaaatt gctgacgtgc agctgccgca aggcgtctgg
    26701 catgaagatg agttttacgg caaaagcgag atttaccgcg atcggctgac gcttcccgta
    26761 accatcaacc aggcgagtgc gggagcaacg ttaactgtca cctaccaggg ctgtgctgat
    26821 gccggtttct gttatccgcc agaaaccaaa accgttccgt taagcgaagt ggtcgctaac
    26881 aacgaagcgt cacagcctgt gtctgttccg cagcaagagc agcccaccgc gcaattgccc
    26941 ttttccgcgc tctgggcgtt gttgatcggt attggtatcg cctttacgcc atgcgtgctg
    27001 ccaatgtacc cactgatttc tggcatcgtg ctgggcggta aacagcggct ttccactgcc
    27061 agagcattgt tgctgacctt tatttatgtg caggggatgg cgctgactta cacggcgctg
    27121 ggtctggtgg ttgccgccgc agggttacag ttccaggcgg cgctacagca cccatacgtg
    27181 ctcattggcc tcgccatcgt ctttaccttg ctggcgatgt caatgtttgg cttgtttact
    27241 ctgcaactcc cctcttcgct gcaaacacgt ctcacgctga tgagcaatcg ccaacagggc
    27301 ggctcacctg gcggtgtgtt tattatgggg gcgattgccg gactgatctg ttcaccatgc
    27361 accactgcac cgcttagcgc gattctgctg tatatcgccc aaagcgggaa catgtggctg
    27421 ggcggcggca cgctttatct ttatgcgctg ggcatgggcc tgccgctgat gctaattacc
    27481 gtctttggta accgcttgct gccgaaaagc ggcccgtgga tggaacaagt caaaaccgcg
    27541 tttggttttg tgatcctcgc actgccggtc ttcctgctgg agcgagtgat tggtgatata
    27601 tggggattac gcttgtggtc ggcgcttggt gtcgcattct ttggctgggc ctttatcacc
    27661 agcctacagg ccaaacgcgg ctggatgcgc gtggtgcaaa taatcctgct ggcagcggca
    27721 ttggttagcg tgcgcccact tcaggattgg gcatttggtg aaacacatac cgcgcaaact
    27781 cagacgcatc tcaactttac acaaatcaaa acagtagatg agttaaatca ggcgctcgtt
    27841 gaagccaaag gcaaaccagt gatgttagat ctctatgccg actggtgcgt cgcctgtaaa
    27901 gagtttgaga aatacacctt cagcgacccg caggtgcaaa aagcgttagc agatacggtc
    27961 ttacttcagg ccaacgtcac tgccaacgac gcacaagatg tggcgctgtt aaagcatctt
    28021 aatgtccttg gcctaccgac aattctcttt tttgacggac aaggccagga gcatccacaa
    28081 gcacgcgtca cgggctttat ggatgctgaa accttcagcg cacatttgcg cgatcgccaa
    28141 ccgtgaacga cacttgcagt gggaaagacg gaggaaaata gcgtgcaacg tgaagatgta
    28201 ctgggagaag ccctgaaatt attagaatta caagggattg ccaacaccac gctggagatg
    28261 gttgctgaac gtgtggatta tccactggac gagctacgcc gcttctggcc agacaaagag
    28321 gcaatcctct acgatgcgct gcgttatctt agccaacaga tagatgtctg gcgtcgtcag
    28381 ctgatgctgg acgaaacgca aaccgccgaa caaaagttgc tggcacgtta tcaggcgtta
    28441 tcggagtgcg ttaaaaacaa ccgctatccg ggctgtctgt ttatcgctgc ctgtacgttt
    28501 taccccgatc ctggccaccc tattcatcaa ctggccgatc agcaaaaaag cgcggcctac
    28561 gatttcaccc acgaactgtt aaccacgctg gaagttgacg atccggcgat ggtagcaaag
    28621 cagatggaac tggtgctgga aggctgttta agccgaatgc tggtgaatcg tagccaggcg
    28681 gatgtcgaca ccgcacatcg gctggcggaa gatatcctgc gcttcgcccg ctgccgtcag
    28741 ggtggtgcac tgacctgaca gaaacacaga aaagaagcga tttgccgcaa tcttaagcag
    28801 ttgaatcgct tttactgaaa ttaggttgac gagatgtgca gattacggtt taatgcgccc
    28861 cgttgcccgg atagctcagt cggtagagca ggggattgaa aatccccgtg tccttggttc
    28921 gattccgagt ccgggcacca aattcatatc aacggacctc cacggaggtc cgtttttcgt
    28981 tccaggacgc cacgatttaa gcgtcctgcc tccaaatcaa ttctaccgaa ctcaaccaga
    29041 ttccccccac atcaacctca ttgtgtgggt ataattgtgg gtatacccca gttcgacaga
    29101 atttgtaccc tctttaacgc attcagaagg ctgaactatg gcactgactg acgcaaaaat
    29161 ccgggcagca aagcctactg acaaggctta taaactcact gacggggcta gcatgttcct
    29221 gctggtacat cctaatggtt cccgttactg gcgtctccgt tatcgtattc tgggtaagga
    29281 gaaaactctg gcacttggtg tgtatccaga agtttctctt tctgaagctc gtacaaaacg
    29341 ggataaggcc cgaaaactga tttcggaggg gattgaccct tgcgaacaga aaagaactaa
    29401 aaaagtagtc cctgatttac aactatcttt tgaacatatt gcacgacgct ggcatgccag
    29461 taataaacaa tgggcacaat cacacagcga taaagtactc aaaagcctcg agacacacgt
    29521 tttccccttt atcggcaacc gagatatcac aacactcagt accccggacc tgcttatccc
    29581 tgttcgtgct gcagaagcta aacaaattta tgaaatcgcc agtcgtctgc agcaaagaat
    29641 atctgctgta atgcgttatg ccgtacagtc tggcatcatc agatataacc cggccctgga
    29701 tatggctggt gcattgacca cggtaaaacg caagcatcgt cctgcactgg atctttcacg
    29761 cctgcctgaa cttctgtcgc gtattgacag ttataaaggc cagcctgtca cccggctggc
    29821 agttatgctg aatttactgg tttttattcg ctccagtgaa ctcagatatg cccgctggtc
    29881 tgaaattgat attgacaatg ccatgtggac tattccagcc gaacgcgaac ctctgccagg
    29941 cgtaaaattc tcacaccgag gctccaaaat gcgaacccca catcttgtgc cactcagcaa
    30001 acaggctgta gccatactaa cagaacttca gacatgggct ggtgaaaatg gtctgatctt
    30061 tacgggagcg catgacccac gtagaccaat cagtgaaaac accgtaaata aagccctgag
    30121 ggttatggga tatgacacaa cccaagaagt ctgtggtcat ggattccggg cgatggcatg
    30181 tagtgcgctg atcgaatcag gtttgtggtc ccgcgatgct gttgaacgtc agatgagcca
    30241 tcaggaacgt aacggtgtac gcgctgctta cattcataaa gcagaacatc tggaagaacg
    30301 ccgattgatg ctacaatggt gggccgattt tctggatgca aacagagaaa aatttatcag
    30361 tccatttgaa tatgcaaaaa ttaacaatcc attaaaatag taatcatccc ggggaaatgc
    30421 ccgggaatta ttttcaatat ctttttcttt gagagtaatg aataagccag acaatggaca
    30481 gaaccatttt ctttacgtca tgtaatcaaa ctgtttatag ccgatatcag ggatatctga
    30541 ttctcccagt agcacgtaat acctcgtcag gtattttcgt gtgttggtaa tatatccaat
    30601 tagtagaaca tgaatttttc gatgaaagca ccaataacct tttcatataa ctcaacagag
    30661 cgtcagaaac agatagcccg gcggccagac gcttgatgct ggttctcagt gtcaggtgtt
    30721 ccgctcaata cgctgagcta atattttacc actcagatgc ttctcgttcg gaatttctct
    30781 ggcataacag tcccagtcat cactggttat cataccgata ttaagcgatg tgagcagagc
    30841 taagcaactc acggcaggtg tcatctataa agggccaaag gtatacacca gaacgccacc
    30901 tattttggtg ttgtaggcat accaaaacca atgttgccag actttactgc cgacaaaact
    30961 ccattgctca tcgaattcgc agatgagtgc cacatcggca ttagcaaccg gagatgatgt
    31021 tattcgcttc ggcgcaagtt ttttaaagtg cgaatgacgg tgttaatacc gtttttaagc
    31081 gtccttgccg tattccggac accggttcca ttgaatacaa ttttggtatt tttttgacac
    31141 tgggcttacg ggattcatag gtataaatga gtataaacac gcgatggcag tctcgaaagc
    31201 gctcacggcc tttagagctc acctcatggc ggtaaatctg agcagactga caacggggac
    31261 aatgaacgat aacgttgacc ataagaaaac ctcaaaagtg ggtattatac accagt
//
LOCUS       DADTFG010000044        30215 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.044, whole genome shotgun sequence.
ACCESSION   DADTFG010000044 DADTFG010000000
VERSION     DADTFG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.044"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..570
                     /locus_tag="J1138_001857"
     CDS             <1..570
                     /locus_tag="J1138_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA8453663.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIH
                     PIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            800..1183
                     /gene="secE"
                     /locus_tag="J1138_001858"
     CDS             800..1183
                     /gene="secE"
                     /locus_tag="J1138_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBA8453664.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1185..1730
                     /gene="nusG"
                     /locus_tag="J1138_001859"
     CDS             1185..1730
                     /gene="nusG"
                     /locus_tag="J1138_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBA8453665.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1889..2317
                     /gene="rplK"
                     /locus_tag="J1138_001860"
     CDS             1889..2317
                     /gene="rplK"
                     /locus_tag="J1138_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBA8453666.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2321..3025
                     /gene="rplA"
                     /locus_tag="J1138_001861"
     CDS             2321..3025
                     /gene="rplA"
                     /locus_tag="J1138_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBA8453667.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3317..3814
                     /gene="rplJ"
                     /locus_tag="J1138_001862"
     CDS             3317..3814
                     /gene="rplJ"
                     /locus_tag="J1138_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBA8453668.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3881..4246
                     /gene="rplL"
                     /locus_tag="J1138_001863"
     CDS             3881..4246
                     /gene="rplL"
                     /locus_tag="J1138_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBA8453669.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4566..8594
                     /gene="rpoB"
                     /locus_tag="J1138_001864"
     CDS             4566..8594
                     /gene="rpoB"
                     /locus_tag="J1138_001864"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBA8453670.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8671..12894
                     /gene="rpoC"
                     /locus_tag="J1138_001865"
     CDS             8671..12894
                     /gene="rpoC"
                     /locus_tag="J1138_001865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBA8453671.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13136..14269)
                     /gene="thiH"
                     /locus_tag="J1138_001866"
     CDS             complement(13136..14269)
                     /gene="thiH"
                     /locus_tag="J1138_001866"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBA8453672.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIAKESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(14266..15036)
                     /gene="thiG"
                     /locus_tag="J1138_001867"
     CDS             complement(14266..15036)
                     /gene="thiG"
                     /locus_tag="J1138_001867"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBA8453673.1"
                     /translation="MLRIADKTFASHLFTGTGKFASSQLMVEAIRACGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVESGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15038..15238)
                     /gene="thiS"
                     /locus_tag="J1138_001868"
     CDS             complement(15038..15238)
                     /gene="thiS"
                     /locus_tag="J1138_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBA8453674.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15222..15977)
                     /gene="thiF"
                     /locus_tag="J1138_001869"
     CDS             complement(15222..15977)
                     /gene="thiF"
                     /locus_tag="J1138_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBA8453675.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLMA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15970..16605)
                     /gene="thiE"
                     /locus_tag="J1138_001870"
     CDS             complement(15970..16605)
                     /gene="thiE"
                     /locus_tag="J1138_001870"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBA8453676.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16605..18500)
                     /gene="thiC"
                     /locus_tag="J1138_001871"
     CDS             complement(16605..18500)
                     /gene="thiC"
                     /locus_tag="J1138_001871"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBA8453677.1"
                     /translation="MSATKLTRREQRALAQHFIDTLEGSAFPNSKRIYITATQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18581..18681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18733..19209)
                     /locus_tag="J1138_001872"
     CDS             complement(18733..19209)
                     /locus_tag="J1138_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HBA8453678.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGVKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            19304..20077
                     /gene="nudC"
                     /locus_tag="J1138_001873"
     CDS             19304..20077
                     /gene="nudC"
                     /locus_tag="J1138_001873"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBA8453679.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20117..21181
                     /gene="hemE"
                     /locus_tag="J1138_001874"
     CDS             20117..21181
                     /gene="hemE"
                     /locus_tag="J1138_001874"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBA8453680.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21191..21862
                     /gene="nfi"
                     /locus_tag="J1138_001875"
     CDS             21191..21862
                     /gene="nfi"
                     /locus_tag="J1138_001875"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBA8453681.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21905..22495
                     /locus_tag="J1138_001876"
     CDS             21905..22495
                     /locus_tag="J1138_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBA8453682.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22682..22954
                     /gene="hupA"
                     /locus_tag="J1138_001877"
     CDS             22682..22954
                     /gene="hupA"
                     /locus_tag="J1138_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBA8453683.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22967..23662
                     /locus_tag="J1138_001878"
     CDS             22967..23662
                     /locus_tag="J1138_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBA8453684.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23664..24083)
                     /gene="zraP"
                     /locus_tag="J1138_001879"
     CDS             complement(23664..24083)
                     /gene="zraP"
                     /locus_tag="J1138_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBA8453685.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGDCGGGGHMGMGHW"
     gene            24321..25697
                     /gene="zraS"
                     /locus_tag="J1138_001880"
     CDS             24321..25697
                     /gene="zraS"
                     /locus_tag="J1138_001880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBA8453686.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGNEEAWRRIDSADGEPVLEIYRQFQPMFATGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERASAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25694..27019
                     /gene="zraR"
                     /locus_tag="J1138_001881"
     CDS             25694..27019
                     /gene="zraR"
                     /locus_tag="J1138_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBA8453687.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKTLNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEIPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27016..28305)
                     /gene="purD"
                     /locus_tag="J1138_001882"
     CDS             complement(27016..28305)
                     /gene="purD"
                     /locus_tag="J1138_001882"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBA8453688.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTTLEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVRDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28317..29906)
                     /gene="purH"
                     /locus_tag="J1138_001883"
     CDS             complement(28317..29906)
                     /gene="purH"
                     /locus_tag="J1138_001883"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBA8453689.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
                     DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGENKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7066 a         7832 c         8340 g         6977 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggtc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      421 gaaggtgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      481 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      541 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      601 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      661 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      721 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaat
      781 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      841 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtagcgattg tcggcaacta
      901 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      961 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagca accgttgctt ttgcccgtga
     1021 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
     1081 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
     1141 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
     1201 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
     1261 tgcgtgagca tatcaaatta cacaacatgg aagagttgtt tggtgaagtc atggtaccaa
     1321 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1381 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1441 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1501 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1561 cgttgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1621 gcgttgttga agaagttgat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1681 gtcgtgcgac ccctgtagag ctggacttca gccaggttga aaaagcttaa cccagcgatc
     1741 aaaaaagcgg cgatttaatc gttgcacaag gcgcgagatt ggaatacaat ttcgcgcctt
     1801 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1861 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1921 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1981 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     2041 aggtctgccg attccggtag taatcactgt ttacgctgac cgttctttca ctttcgttac
     2101 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     2161 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     2221 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     2281 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     2341 catgcgtgtt atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2401 cgctctgctg aaagaactgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2461 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2521 cggtactggc cgttccgttc gcgtagccgt atttacccag ggtgcaaacg ctgaagctgc
     2581 taaagctgca ggtgctgaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2641 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2701 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2761 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2821 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2881 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2941 gtacatcaag aaagttagca tctctaccac catgggtgca ggtgttgcag ttgaccaggc
     3001 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     3061 tacccccacg tatgctaacg aaagttagca gctaaaaaga tttgttcgtt ggagcctggc
     3121 ctatccaggc ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag
     3181 acggtgacag aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat
     3241 taagacgctc tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca
     3301 tccaggagca aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt
     3361 cagcgaagta gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt
     3421 agataaaatg actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt
     3481 tcgtaacacc ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc
     3541 gtttgttggt ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct
     3601 gttcaaagag ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg
     3661 tgagctgatc ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc
     3721 aattgcacgc ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct
     3781 ggctgctgta cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg
     3841 cttacgtata aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca
     3901 aatcattgaa gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat
     3961 ggaagaaaaa ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga
     4021 agctgctgaa gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt
     4081 tgctgttatc aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct
     4141 ggttgaatct gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact
     4201 gaaaaaagct ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg
     4261 ttgcagcctg agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc
     4321 tgtaaggcgc cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt
     4381 tctatcgacg acttaatata ctgcgacagg acgcccgttc tgtgtaaatc gcaatgaaat
     4441 ggtttaagcg tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat
     4501 agtgttgcac aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga
     4561 accctatggt ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc
     4621 cacaagttct ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta
     4681 tcgagcaaga tcctgaaggg cagtatggtc tggaagctgc tttccgttcc gtattcccga
     4741 ttcagagcta cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg
     4801 tgtttgacgt ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta
     4861 aactgcgtct ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag
     4921 aacaagaagt ctacatgggc gaaattccgc tcatgacgga caacggtacc tttgttatca
     4981 acggtactga gcgtgttatc gtttcccagc tgcaccgtag tccgggggtc ttttttgact
     5041 ccgacaaagg taaaacccac tcttcgggta aggtgctgta taacgcgcgc atcatccctt
     5101 accgtggttc ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg
     5161 accgtcgccg taaactacct gcgacaatca ttctgcgtgc cctgaactac accacagagc
     5221 agatcctcga cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagttgcaga
     5281 tggaactggt gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg
     5341 gtaaagtgta cgtagaaaaa ggccgccgca ttactgcgcg ccatattcgc cagctggaaa
     5401 aagacgacgt caagctgatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta
     5461 aagactacat tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc
     5521 tggatctgct ggctaagctg agccagtctg gtcataagcg tatcgaaacg ctgttcacca
     5581 acgatctgga tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc
     5641 gtctgagcgc actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg
     5701 aagcagctga aagcctgttc gagaacctgt tcttctccga agaccgttat gacctgtctg
     5761 cggttggtcg tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta
     5821 tcctgagcaa agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta
     5881 aaggcgaagt cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa
     5941 tggcggaaaa ccagttccgc gttggcctgg tacgtgtaga gcgtgcggtg aaagagcgtc
     6001 tgtctctggg cgatctggat accctgatgc ctcaggatat gatcaacgct aagccgattt
     6061 ccgcagcagt gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca
     6121 acccgctgtc tgaaattacg cacaaacgtc gtatctccgc actcggcccg ggtggtctga
     6181 cccgtgaacg tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat
     6241 gtccaatcga aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg
     6301 cacagactaa cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg
     6361 taactgacga aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg
     6421 cgaactccaa cctggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca
     6481 aaggcgaatc cagcttgttc agccgtgacc aggttgacta catggacgta tccacccagc
     6541 aggtggtatc cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg
     6601 cattgatggg tgcgaacatg caacgtcagg ccgttccgac tctgcgtgct gataagccgc
     6661 tggttggtac gggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggttgcta
     6721 aacgtggtgg tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag
     6781 acgagatgta tccgggtgaa gcaggtatcg acatctacaa cctgaccaag tacacccgtt
     6841 ctaaccagaa cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggtagaac
     6901 gtggcgacgt gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga
     6961 acatgcgcgt agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat
     7021 ccgagcgtgt tgttcaggaa gaccgtttca ccaccatcca tattcaggaa ctggcgtgtg
     7081 tgtcccgtga taccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg
     7141 aagctgcgct ctccaaactg gatgaatccg gtatcgttta cattggtgcg gaagtgaccg
     7201 gtggcgacat tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag
     7261 agaaactgct gcgtgcgatc ttcggtgaga aagcgtctga cgttaaagac tcttctctgc
     7321 gcgtaccaaa tggtgtatcc ggtacggtta tcgatgttca ggtctttact cgcgatggcg
     7381 tagaaaaaga caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag
     7441 acctgtctga agaactgcag atcctcgaag caggtctgtt cagccgtatc cgtgctgtgc
     7501 tggtagccgg tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg
     7561 agctgggcct gaccgacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg
     7621 acgaactgaa acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg
     7681 gcgacgatct ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc
     7741 gtatccagcc tggtgacaag atggcaggcc gtcatggtaa caagggtgta atttctaaga
     7801 tcaacccgat cgaagatatg ccttacgatg aaaacggtac tccggtagac atcgtactga
     7861 acccgctggg cgtaccgtct cgtatgaaca ttggtcagat cctcgaaact cacctgggta
     7921 tggctgcgaa aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg
     7981 cgaaactgcg cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag
     8041 ttgacctgag taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag
     8101 gtatgccaat cgcaacgccg gtcttcgacg gtgcgaaaga agcagaaatt aaagagctgc
     8161 tgaaacttgg cgacctgccg acttctggtc agatccgcct gtacgacggt cgcactggtg
     8221 aacagttcga gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg
     8281 tcgacgacaa gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc
     8341 tgggtggtaa ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg
     8401 aagcatacgg cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga
     8461 acggccgtac taagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca
     8521 tgccagaatc cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac
     8581 tggaagacga gtaattctcg ctcaaacagg tcactgctgt cggggtaaaa cccggcagcg
     8641 gattgtgcta actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg
     8701 cagactaaaa ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc
     8761 cgttcatggt ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa
     8821 ccagaacgtg acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc
     8881 ctgtgcggta agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt
     8941 gaagtgaccc agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg
     9001 actgcgcaca tctggttcct gaaatcgctg ccgtcccgta tcggcctgct gctcgatatg
     9061 ccgctgcgcg atatcgaacg tgtactgtac ttcgaatcct atgtggttat cgaaggtggt
     9121 atgaccaacc tggagcgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa
     9181 gagttcggtg acgaattcga cgcgaagatg ggtgcggaag caatccaggc cctgctgaag
     9241 agcatggatc tggagcaaga gtgcgaacag ctgcgtgaag agctgaacga aaccaactcc
     9301 gaaaccaagc gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct
     9361 ggtaacaaac cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt
     9421 ccgctggttc cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt
     9481 cgcgtcatta accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggatatc
     9541 atcgtacgta acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt
     9601 cgtcgcggtc gtgcaatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg
     9661 atcaaaggta aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc
     9721 ggtcgttctg taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag
     9781 aaaatggcgc tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt
     9841 gctaccacca tcaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat
     9901 atcctggacg aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac
     9961 cgtctgggta tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac
    10021 ccgctggttt gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta
    10081 ccgctgacgc tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc
    10141 ctgtccccgg caaacggcga accaatcatc gttccgtctc aggacgttgt actgggtctg
    10201 tactacatga cccgcgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg
    10261 aaagaagcag aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg
    10321 cgtatcaccg agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa
    10381 gacacgactg ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc
    10441 atcgtcaacc aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc
    10501 attctcggtc tgaaaccgac cgttattttt gcggaccaga tcatgtatac cggctttgca
    10561 tatgcagcgc gttctggtgc atctgttggt atcgatgaca tggtcatccc ggagaagaaa
    10621 cacgaaatca tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct
    10681 ggtctggtaa ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac
    10741 gatcgtgtat ccaaagcgat gatggataac ctgcaaactg aaaccgttat taaccgtgac
    10801 ggtcaggaag agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg
    10861 cgtggttctg cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg
    10921 gatggctcca tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc
    10981 cagtacttca tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaacg
    11041 gcgaactccg gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc
    11101 gaagacgatt gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgac
    11161 gttaaagagc cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag
    11221 ccgggtactg ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac
    11281 ctgctggaag agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc
    11341 gactttggtg tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac
    11401 aaaggtgaag caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg
    11461 accatgcgta cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc
    11521 caggtgaaaa acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc
    11581 ggtaaactgg ttatcacttc ccgtaacacc gaactgaaac tgatcgacga attcggtcgt
    11641 accaaagaaa gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag
    11701 gttgctggcg gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa
    11761 gtaagcggtt ttgtacgctt tactgacatg atcgacggcc agaccattac tcgtcagacc
    11821 gacgaattga ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt
    11881 ggtaaagatc tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatc
    11941 ccgggtaccg atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa
    12001 gatggcgtac agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt
    12061 accaaggaca tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg
    12121 aaagagccgg caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa
    12181 ggtaaacgtc gtctggttat caccccggta gacggtagcg atccgtacga agagatgatt
    12241 ccgaaatggc gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt
    12301 tccgacggtc cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgtgact
    12361 cgttacatcg ttaacgaagt acaggacgta taccgtttgc agggcgttaa gattaacgat
    12421 aaacacatcg aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc
    12481 agctccgact tcctggaagg tgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc
    12541 gaactggaag cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc
    12601 aaagcgtctc tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc
    12661 gtgctgaccg aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac
    12721 gttatcgtgg gtcgtctgat cccggcaggt accggttatg cgtaccacca ggatcgtatg
    12781 cgtcgccgtg ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct
    12841 gccagcctgg cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta
    12901 atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggtgtt attcagaggg
    12961 caaagtcttc acgggaaata ttaagaaagg cagggcgaaa cgaggaagaa gcttttcgcc
    13021 ctggacaaca ttcctgttga cgggactacc ataaaaaaac acaacgcttt caacaacctt
    13081 gcgtttttca tcttccggag ctgctcggaa taaatttacg ttcttacatt ctgtctcatg
    13141 gcctttgcga tgggcgtccc agatagctgt cccagtcttt ccataccggc tgcaaaccct
    13201 gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt gagaactgtt
    13261 ccagctcggg gtggttgtcg gcatagccac ctggctgcgt tttcgaaaaa gcgctgacgt
    13321 tattaattgc cagcggaatt acgcgatcgc gaaaccacgg tgattcccgc gtggagagtg
    13381 acagttcaat ctccggtgca agcagccgga aagcgcagat ggcttgcact aactggcgtt
    13441 catccataat cgacgcaggc tcaatgccgc cagtacatgg acgcaggcgt gggaaggaga
    13501 cagagtagcg gctttgccag taatgctgtt gcagccatag caaatgttcg gcaaccatat
    13561 agcagtcaac tcgccagctg tcggaaaggc cgattagcgc gccgaggcct atcttatcaa
    13621 tccccgcacg ccctagccga tccggcgttt ccagccgcca gaagaagtcc tgttttttac
    13681 ctttcagatg atgatgggca taagtcgcct catgatatgt ctcctgataa accattactc
    13741 catcgaggcc aagctgtttt aactcggcat attccgcctc cgccagcggt tgcacttcca
    13801 tctgtagtga agagaactgt tcacgcaggg cagggagatg acgacgaaag taatccatcc
    13861 ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc atctcccgta
    13921 tagcggcgct ttccttggca atatccgctt catccagcgt tttgcgcttg atgcggttgc
    13981 tcatagaaaa tccgcaataa gtacagtcgt tagcgcaaag attggaaaga taaagcggga
    14041 cgtagaagct gacgacgttg ccaaatcgct gacgagtcag acgttgcgct cgctgggcca
    14101 gtggttccag atagccactg gcggcgggtg atagcaatgc catcatgtcg tcgcgggtga
    14161 gttgcgaggc atttagcgcc cgctctacgt cagcagccgt tttgccgttg atacgcaggc
    14221 ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg ccgatgcctc
    14281 cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc ccggtccgga
    14341 ctgacgtgcc agcaggcctg attctaccgc cagacgaaat gccttcgcca tgttgacggg
    14401 atcgtccgcg acggcaatcg ccgtattcac taacaccgcg tcggccccca tttccagcgc
    14461 ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca accaccggaa cggtggcctg
    14521 ctggataata atctccagca tggcgcgggt ttccagtccc tgattcgagc caatcggcgc
    14581 gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca ataccggatc
    14641 ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcgtttcgg cggctttcag
    14701 ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcca attttagcca
    14761 gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt ccgcggtttt
    14821 cgccccggaa gtattcggca gcagggtcac acccgccgcg ataagcggtt cgaggatagc
    14881 gtcgttgtgc tggcgcaagt cgacacgttt catcgccagc gtcaccagtt ggctgccgca
    14941 agcgcggatc gcctccacca tcagttgtga agaggcgaat tttccggtgc cagtaaacag
    15001 atgtgaagca aacgttttgt ccgcaatacg taacatctta accccctgca ataacctgaa
    15061 aaagcaggat ctggtcgcca tcctgcacga tatgttgcgc ccactgctca cgcgggacga
    15121 tttgctgatt aatcgccagt gccgcgcccg cttgttgttg gtcgagttgc tccagtagtt
    15181 cgtgaaccgt ttgcccggcg gcgcactgca tcggttgatc gttaaacagg atctgcattg
    15241 cttcctccgc ataccgggca accactggcg cggcgcaaag ccaggctgcg ccactggctc
    15301 gacttaccgt cgaacagtcg gagttctccc gcaggcgtct ctataccact taataactta
    15361 atcgcttcca gtgcctgtaa tgtgcccata accccgacca ccgggccaac cacgcccgcc
    15421 gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta acacccctgc
    15481 tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct ggcggtgata
    15541 agcggcgtgt tgagtgccac gcaggtggca ttaatctcct ggcgagtcgc catattgtcg
    15601 gtacagtcga gcaccacatc ggcccgtgct accgcatctt ttaacgcctc acccgtcaac
    15661 cgttgttgta atgccatcag ttgaatgtcg ggattcaact gtgtcagtcg ctgttggctg
    15721 acctgtgatt tcgggcgatc gatatcttca gtggtaaaga ggatttgtcg ttgcagattg
    15781 cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc ccccgccagg
    15841 tatagcgcag caggtgtacc cagcccgcca agaccaataa tcagcacctg gctgtcgagc
    15901 agtttttgct gcccgtccag agcgatatcg tcgagcagga tttggcggct ataacgcata
    15961 aagtcacggt cattcatcgc caactcctgc aatttccagc agctgtgccg ttgccaaacg
    16021 ccagtctgcg gcttgagtaa tggcgctgac gacggcgata ctgccgacac ccgttgctat
    16081 caccgcaggc gcgcgcgcca gactgatacc gccaatcgcc acggtgggat aatccgccag
    16141 gcgctcaaca tgccgtgcca gctgttccag tccctgcggt gcagaaggca tctgtttggt
    16201 ttgtgtcgga aacacatgtc ccagcgcgat ataagaagga cgtgccgcca gcgcgacgtc
    16261 gatctccata tcgtcatgag tcgaaacgcc cagccgcagg cccgctgcac ggatggcatt
    16321 gagatcggtg gcttgcaaat cttcctgccc cagatggacg ccatacgcct gatgcttaat
    16381 cgccagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc ccagcgcaat
    16441 tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc gtagctggag
    16501 agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcaa ccaccgggta
    16561 cagtcctaaa cgaaagggta caggaggaaa ctcaggctga tacatcacgc ttcctcctta
    16621 cgcaggtaga tttctccgcc tctggcgcgg aagttctccg acatatccgc cattcccact
    16681 tcaatggttt gcgcggcggc gtaatcacgc acttcctggc tgattttcat cgagcagaat
    16741 ttcggtccac acatggagca aaaatgggcg actttaccgg actcttgcgg cagggtttca
    16801 tcgtgataag cgcgggcagt aaacgggtcg agggccagat taaactggtc ttcccagcga
    16861 aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg atgccctttc
    16921 gccaggtcgg cggcgtgggc ggcaatctta taggtgataa gcccctgttt aacatcttct
    16981 ttattaggca gacccagatg ctcttttggc gttacgtagc agagcattgc acaaccaaac
    17041 cagccaatca tcgccgcgcc aatccctgac gtgaagtggt catagcccgg cgcgatatcg
    17101 gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa ctcctcggtc
    17161 atattgcggc gaatcatctg catcggcacg tggcctgggc cttcaatcat cacctgcacg
    17221 tcatattccc aggcgatttt ggtcagttcg cccagcgtat gcagctcggc aaactgtgct
    17281 tcatcgttgg cgtcctgaat agaaccgggg cgcaggccgt cgcccagcga cagcgaaacg
    17341 tcataagcag cacagatttc acaaatttcg cggaagtgtt gatagaggaa attttcctga
    17401 tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat accggtcagg
    17461 cgtttcgcgg tcatcggcac gtagcgcagc agcacccccg catggatagt gaagtaatcc
    17521 acaccttgct cggcttgttc cagcagggtg tcgcggaaga cttcccaggt aagatcttca
    17581 gcgatcccgt tcaccttctc cagtgcctgg tagatcggca ctgtaccgat cggcaccggg
    17641 ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccagtgga aagatccatc
    17701 acggtatccg ctccccagcg tgtggaccat accagttttt ccacttcttc ttcgatggaa
    17761 gaagtgaccg ctgagttgcc gatattggcg ttaactttta ccaggaagtt acggccaata
    17821 atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg tccggcagca
    17881 acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc aaagctcatt
    17941 cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcgacccat attctcgcga
    18001 atggcgatga attccatttc gggcgtgatg ataccctggc gggcgtagtg cagttgggtg
    18061 acgcggcgtc ctgctttggc gcgttttggc gttaatacgc cgctaaaacg cagctcgtcg
    18121 aggccatcat ctgccagccg cgcttttgtg taatcggaac tgcgcacggt aagttcttcg
    18181 gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg ctgcacgtta
    18241 atggcgatct gcggatcgcc atacggaccg gaggtgtcgt agaccggaat cgcttcgttt
    18301 tcttcgtact gcggttgttc tttgctaccg ccgattagcg tcgggctaag ctggatctca
    18361 cgcatcggca cgcgcacgcc gggttgtgtg gcagtgatgt aaatgcgttt tgagttggga
    18421 aaggcggagc cttccagggt atcgataaaa tgttgggcca gggcgcgttg ttcgcggcgg
    18481 gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct tgtcagacga
    18541 cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa ctcttgttcc
    18601 cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc tcagccagtt
    18661 aaggcactcc gacaagaaat tagccccaca aacggggcgt tgaatgtaaa ttacgcgtta
    18721 acagcgcaga actcaagcag gatatttgac gcgggcggcg tcaagcacca gtagaatcag
    18781 cttatcttcc agcacaaagc gcgcttccag tgcctcgccg atgtcagata aaacctgttg
    18841 aaactcaagg taattatcat gatcgatggc ggtttccaga ctggaatcgt agtcatccat
    18901 aatctgttga gtattggctt cgagttgtgg ccaaatcttc gcggcgcgtg cgagttgccc
    18961 attgccttcc agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta
    19021 atcgaccaag ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc
    19081 tttgccaggc ttaacgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg
    19141 tagccagcga tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg
    19201 gttaagcatg attgactccg caagtttgta ttcaaaaact gctctgtgag aaatgtaaaa
    19261 accatgttaa acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga
    19321 aaaattagat cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg
    19381 agaattgcca tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat
    19441 cggcgaatgg cagggggaac ctgtttggtt agtacaactg cagcggcgtc acgatatggg
    19501 gtcggtacgt caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt
    19561 gcaactggcg gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta
    19621 tccaagcaaa accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca
    19681 aatcgccccc tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca
    19741 tacccgccat cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggtgaaac
    19801 cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt
    19861 gcgttacgtg acctcccaac cgtggccgtt tcctcagtcg ttaatgaccg cgtttatggc
    19921 ggaatatgac agcggcgaga tcgtgatcga cccgaaagaa ttgcttgagg cgaactggta
    19981 tcgctatgac gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga
    20041 agatacagtg gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca
    20101 ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg
    20161 ccagcccgtt gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga
    20221 atataaagcc acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa atgccgagct
    20281 ggcgtgcgaa gtgactttgc aaccgctgcg tcgctatccg ctggatgcgg cgatcctctt
    20341 ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga
    20401 aggtccgcgt tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc
    20461 ggacccggaa gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcgcgaact
    20521 gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg tggacgctgg cgacttacat
    20581 ggttgaaggc ggtagcagca aagccttcac cgtgatcaaa aagatgatgt atgccgatcc
    20641 gcaggcgctg cacgcgctgc tcgataaact ggcgaaaagc gtcacgttgt atctgaatgc
    20701 gcagattaaa gccggtgctc aggcagtcat gattttcgac acctggggcg gcgtgcttac
    20761 cgggcgcgat tatcaacagt tctcgcttta ttacatgcat aaaattgttg atggtttact
    20821 gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt accaaaggtg gcggacagtg
    20881 gctggaagcg atggcagaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat
    20941 cgccgatgcg cgccgtcgcg tgggcaataa agtcgcgctg cagggtaata tggacccgtc
    21001 gatgctgtac gcgccgcctg cccgcattga agaagaagtg gcgtctatac ttgccggttt
    21061 cggtcacggc gaaggccatg tctttaacct tggtcacggc attcatcagg atgtgccgcc
    21121 agaacatgca ggcgtgtttg tggaggcagt gcatcgcttg tctgaacagt atcaccgcta
    21181 aggagcgatt atggatctcg cgtcattacg cgctcaacaa attgaactgg cttcttctgt
    21241 gatccgcgag gatcgactcg ataaagatcc gccggatctg atcgccggag ccgatgtcgg
    21301 atttgagcag ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat acccctcgct
    21361 tgagctggtg gagtataaag tggcccgcat cgccaccacc atgccttaca ttccaggttt
    21421 tctctcgttc cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt cgcaaaagcc
    21481 ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct cgccgtcttg gcgtagccag
    21541 ccattttggc ttattggtgg atgtgccgac cattggcgta gcgaaaaaac ggctctgcgg
    21601 taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc ccgctgatgg ataaaggcga
    21661 gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta tcgctaccgg
    21721 ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa cgctgcatga aaggctatcg
    21781 tctgccggag ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg cgttcgtgcg
    21841 ctatacagca aatcagccct aattcaggta aactgcggat aatttccgta tttgagaact
    21901 caacatgtta caaaacccaa ttcatctgcg tctggagcgc ctggaaagct ggcagcatgt
    21961 cacttttatg gcctgcttat gcgaacgcat gtaccccaat tacgccgtat tctgtcagca
    22021 aaccggcttc ggcgatgggc agatttaccg ccgcattctc gatctcattt gggaaacgct
    22081 gaccgtcaaa gacgcaaaag taaatttcga cagccaactg gagaaatttg aagaagcgat
    22141 tccttcagcc gacgatttcg atctgtacgg cgtttatccg gcaattgatg cttgcgtggc
    22201 gttaagtgaa ctggtccatt cgcgtttaag tggcgaaacg ctcgaacacg cggtggaagt
    22261 gagtaagacc tccatcacga ccgtggcgat gctggaaatg actcaggctg gtcgcgaaat
    22321 gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa gaatgggaca ttcagtggga
    22381 aatattccga cttttagctg agtgcgaaga acgcgatatc gagctgataa aaggccttag
    22441 ggcagacctg cgtgaggcgg gtgagagcaa tattggtata atttttcagc aataagacca
    22501 gaaaacgtga tttaacgcct gatttgtcgt acctggagtc ttccctttcg ccccccgtct
    22561 ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag gagagtgcta
    22621 ttctggcatt tccgtcgcac tcgatgctta gcaagcgata aacacattgt aaggataact
    22681 tatgaacaag actcaactga ttgatgtaat tgcagagaaa gcagaactgt ccaaaaccca
    22741 ggctaaagct gctctggagt ccactctggc tgcaattact gagtctctga aagaaggcga
    22801 tgctgtacaa ctggttggtt tcggtacctt caaagtgaac caccgcgctg agcgtactgg
    22861 ccgcaacccg cagaccggta aagaaatcaa aattgccgca gctaacgtac cggcatttgt
    22921 ttctggcaag gcactgaaag acgcagttaa gtaagattgc gtggcagtga acagttttaa
    22981 cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc tggcaggcgc
    23041 tctgtttctc actgcctgta gtcacaactc ttcacttcct ccctttaccg ccagtggatt
    23101 tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac agcggcgata atgtgcatct
    23161 gcttgccgtg tttagcccgt ggcgcagtgg cgataccacg acgcgagagt atcgctggca
    23221 gggcgataac cttacgctca tcaatatcaa tgtttacagc aaaccgccgg tgaacattcg
    23281 cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa cgtgaatccg atggggaaaa
    23341 gcagcagctt tctaacgacc aaatcgattt ataccgttat cgtgctgatc agatccgcca
    23401 gattagcgat gccttacgtc aggggagagt cgtgctgcgc caggggcgct ggcatgcgat
    23461 ggaacagacc gtgaccacct gcgaagggca aaccattaaa cctgatttag attcgcaggc
    23521 gatagcgcat atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg tggcatggct
    23581 ggaagcgccc gaaggttcgc aattactgtt ggtggcaaac tctgatttct gtcgctggca
    23641 acccaacgag aaaacgttct gatttaccag tggcccatac ccatatgacc gccaccaccg
    23701 cagtcgccgt aacccattcc ggtaccgcgc ggaatacccg cttcagccat cgcaatatct
    23761 cgtttcaccc gtaactcatc taacgactga cgcaaattct ccatctcttt ggcgaccgcg
    23821 ttaattttgc tgctatccgg tgggttcgcg gctaacaggg cattgtattc ataacgcttt
    23881 gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa agtcattatg gattttctgc
    23941 cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt gctgccacat accatgtccg
    24001 ccgtgagcaa atgcagatgt cgatcccatc gccattgctg aaagcgccat cattaccagg
    24061 gcaattttcg tgttccgttt catggttaat cctccagtgg ttgtcttact tcgggtattg
    24121 catcttccgt gccaacggcg aaacgctgat atgacgggta atctggcatg ataaacgagt
    24181 aaaaatgact cgcctgctgc gggtagcgag tcatttttac tcattgaaac ttaagccttt
    24241 gtgttacagc tcagggtaag cgctgataaa agatggcatg atttctgctg tcagaaaggg
    24301 atggacaggc aaagaagaag atgcgtttta tgcaacgttc taaagactcc ttagctaaat
    24361 ggttaagcgc gatcctcccc gtggtcattg ttgggctggt ggggctgttt gcggtgacag
    24421 tgattcgcga ttatgggcgc gagactgccg ccgccagaca aacgctgctg gaaaaaggca
    24481 gtgtacttat tcgtgctctt gaatccggct cgcgcgtcgg catggggatg cgcatgcatc
    24541 atgcgcagca gcaggcctta cttgaagaaa tggccgggca gcctggagta cgttggtttg
    24601 cggtcacgga tgaacaagga acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac
    24661 agctttattc cccgcaggaa atgcagcagt tacatccggg aaatgaagaa gcgtggcggc
    24721 ggatcgatag cgcagacggc gagcctgttc tggaaattta tcgccagttt caaccgatgt
    24781 ttgctactgg aatgcaccgg atgcgccata tgcagcagta tgccgcgaca ccacaagcaa
    24841 tttttatcgc tttcgacgcc agtaacattg tgagtgccga agatcgtgag cagagaaaca
    24901 ccctgattat cctcttcgcc ctggcgacgg tcttgctggc aagcgtgttg tcattcttct
    24961 ggtatcgccg atatctgcgc tcgcggcagc tattgcagga tgaaatgaag cgcaaagaga
    25021 agctggtggc actggggcat ctggcggcag gcgttgccca cgaaatccgt aacccacttt
    25081 cctcgattaa agggctggcg aaatactttg ccgaacgcgc gtcagcaggg ggagaagcgc
    25141 atcaattggc gcaggtgatg gcgaaagaag ccgaccgttt aaaccgcgtg gtaagcgagc
    25201 tgctggaact ggttaagcca acgcatctgg ctttgcaggc ggtggagctc aacacgctga
    25261 ttagccactc attacagctg gtaagccagg atgcaaacag ccgggagatc cagttacgct
    25321 ttaccgccaa cgacacatta ccggaaattc aggccgatcc ggacaggctg actcaggtcc
    25381 tgttgaatct ctatctcaac gctattcagg cgattggtca gcatggcgtg attagcgtga
    25441 cggccagcga aagcggcgcg ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg
    25501 cggcggatca gcttgaagcc atcttcactc cgtacttcac caccaaagcc gaaggcaccg
    25561 gactggggct ggcggttgtg cataatattg ttgaacaaca cggtggtaca attcaggtcg
    25621 caagccagga gggaaaaggc gcaacgttca ccctttggct tccggtcaat attacgcgta
    25681 aggacccaca aggatgacgc acgataatat cgatattctg gtggtggatg atgacattag
    25741 ccactgcact attttgcagg ctttgctgcg cggctggggc tataacgtcg cgctggcgaa
    25801 cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt tttgatcttg tgctttgcga
    25861 tgtgcgaatg gcagagatgg atggtatcgc cacgctaaaa gagatcaaaa cattaaaccc
    25921 cgcgattccg gtgctgatta tgaccgcgta ctccagcgtc gagacggcgg tagaggcgtt
    25981 gaaaaccggg gcgctggatt atctcatcaa gccgctggat ttcgataacc tacaggcgac
    26041 gctggaaaag gcgctcgcgc atacacacag tgttgacgct gaaacgcctg cggtgtctgc
    26101 cagccagttc ggtatggtcg gtaaaagccc ggcgatgcaa cacctgctca gtgaaatcgc
    26161 cctcgtcgcg ccatcggaag ccacggtgct gatccacggc gattccggca ccggtaaaga
    26221 gctggtcgcc agggcgattc acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct
    26281 caactgcgcg gcgctcaacg aatccttgct ggagtctgaa ttgttcggtc acgaaaaagg
    26341 ggcgtttacc ggggccgaca aacgccggga agggcgcttt gttgaggcgg acggcggcac
    26401 gttgtttctc gatgaaattg gcgatatctc gccgatgatg caggtgcgtc tgctacgtgc
    26461 gattcaggag cgcgaagttc agcgtgtcgg tagcaaccag actatttcgg ttgatgtccg
    26521 gctgattgcg gcgacccatc gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca
    26581 ggatctctac tatcggctga acgtggtggc gattgaaata ccatcgctgc ggcaacggcg
    26641 ggaagatatt cctctgctgg ctggccattt tcttcagcgc tttgccgaac gtaatcgcaa
    26701 ggcggtaaaa ggctttacgc cccaggcgat ggatctgctg attcattacg actggccggg
    26761 aaatattcgt gaactggaaa acgcggtgga acgggcggtg gtgctgctga ccggggaata
    26821 tatttccgaa cgcgagctgc cgctggcgat tgccagtacg ccgatcccgc tggtacaaag
    26881 tcaggatatt cagccgctgg tggaagtcga aaaagaagtg attctggcag cactggagaa
    26941 aacgggcggc aacaaaaccg aagccgcccg tcagttaggg atcacgcgta aaacgctatt
    27001 ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagcg cgccagccga tatctttccg
    27061 gcagaagcag tcgtcccaat ggatatcggt cattaaggca taagcgcgtt tctgtgcttc
    27121 tgccacggta tgacccagcg cggtaacgca cagtacgcgc ccgccgctgg tcactacctg
    27181 ctcgtcatcc gccagtttcg tgcccgcgtg gaacactttg ccgtctgcca cttcttccag
    27241 cggcaggccg tggatcacat cgccggtgcg gtaatcgccc ggatatccac ccgcagccat
    27301 caccacgccg agagaagcgc gttcatccca ctcggacgtt ttctcgtcca gcttgccttc
    27361 acaggccgcc aggcagagct caaccagatc ggacttcatg cgcagcataa tcggctgggt
    27421 ttccggatcg ccaaagcggc agttaaattc aataaccttc ggattgccct gtttgtcgat
    27481 catcaggccc gcgtagagaa aaccagtgta ggtgttgcct tccgccgcca tgcctttcac
    27541 ggttggccag atgatacgtt ccatcgtgcg ctgatgaacc tcatcggtca ctactggcgc
    27601 aggggagtaa gcgcccatcc cgccggtgtt cggcccggta tctttatcgc ctacgcgttt
    27661 gtgatcctgg ctggtggcca tcggcagcac atgctcgccg tccaccatca cgataaagct
    27721 cgcttcttcg ccgtcgagga actcttcgat aacaatgcga tgacccgcgt cgccaaacgc
    27781 gttgcccgcc agcatgtcgc gcacggcggc ttcagcttct tccagcgtca tcgccacgat
    27841 aacgcctttc ccggcggcca gaccgtccgc tttaatgacg attggcgcgc ctttctcacg
    27901 cagatacgcc agcgcaggtt ctacctcggt gaagttctgg tattccgctg tgggaatgtt
    27961 atggcgggcc agaaagtctt tggtgaatgc tttagaacct tccagttgtg cggcacctgc
    28021 ggttgggccg aagattttca gcccggcggc gcggaaggta tcgactacgc ctttcaccag
    28081 cggcgcttcc gggccgacga tggttagatc taccttttcg ttttgtgcga aatccagcag
    28141 cgccgggata tcggttacgc cgatagcgac gttctgtagc gcgggttcca gcgtagtgcc
    28201 tgcattaccc ggagcaacaa aaacagtttc aaccagcggc gactgggccg ctttccaggc
    28261 cagcgcgtgc tcgcgcccgc cgttaccgat cactaatact ttcatctgtc gctccattaa
    28321 tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca
    28381 attacttcgt catcacggat agaaccgcca ggctggatta cgcaggtcac gcccgcagcg
    28441 gctgcggcat caataccgtc gcggaacggg aagaacgcgt cagaagccat cgaggaacct
    28501 ttcacttcca gaccttcatc ggcagctttg ataccggcga ttttcgcgga gtacacgcgg
    28561 ctcatctggc ctgcgccaat accgatggtc atattgtttt tggcatagac gatagcgttg
    28621 gatttcacga acttcgccac tttccagcag aacagcgcat cacgaagttc ctgttcagtc
    28681 ggctgacgtt tggtgacgac gcgcagttct tccgcaccga ccatccccag atcgcgatcc
    28741 tgaaccagca gaccgccgtt cacgcgtttg aaatcaagac ccggaatacg ttcgccccac
    28801 tgaccgcagg tcagaacgcg tacgttctgc ttggcggcgg tgattttcag ggcttcttcg
    28861 ctggcggacg gcgcaataat cacttcgacg aactgacgag aaatgatggc ctgcgcggtt
    28921 tcagcatcca gttcgcggtt aaaggcaatg atgccgccga atgcggaggt tgggtcggtt
    28981 ttgtacgcgc gatcgtaagc atcaagaatg gaattgccga tagccacgcc gcaagggttg
    29041 gcgtgtttca caatcacaca tgccggctcg gcgaactctt tcacgcactc cagcgccgca
    29101 tcggtatcgg cgatgttgtt ataagagagg gctttaccct gaacctgggt tgcggtggca
    29161 acggaggctt ctttcacatt ctcttctata tagaaggcag cctgctggtg gctgttttcg
    29221 ccgtaacgca tatcctgctt cttaatgaag ttcaggttca gcgtgcgtgg gaagcgaccg
    29281 gcggcttctt tgttttcacc gtggtaagcc ggaaccatgc tgccgaagta gttggcaatc
    29341 atgctgtcgt aggcggcggt gtgttcgaag gctttgatgg caaggtcaaa gcgagtttcc
    29401 agagtcaggg agccttcatt ggcatctatc tctttaataa tggcgtcgta gtcgctgctc
    29461 ttcaccacga tggcgacatc tttatggttc ttggcggcag agcgcaccat cgttgggccg
    29521 ccgatatcga tgttctcaac cgcatcttcc agcgagcaac cttcacgggc aacggtctgg
    29581 gcgaacggat acagattaac aaccaccata tcgataggct ggatctgatg ttcttccata
    29641 atggcatcgt cctggccgcg acggcccaga atgccaccat gtactttcgg atgcagggtc
    29701 ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc
    29761 agaccttttt ctgctaacag acgggctgtg ccccctgtag acagcagctc cacaccgcgt
    29821 gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt cagaaacact gagcagagcg
    29881 cggcggactg gacgacgttg ttgcatggta aatcccctgg atttgactat tacagagagc
    29941 gttagctgaa tttttcgcga aaaactcagc taacgcccct aacggggcat ccttattttt
    30001 cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga atttttctct ttcaatggtg
    30061 atcacaattt tgacagtgtt taccgtggga aaaatacaga aattacattg atgattgtgg
    30121 ataactctgt gcgtaaaaag gtataaagcg gggttttgct ggggaatgca gcagtcagtc
    30181 atttttctgc aatttttcta ttgcggcctg cggag
//
LOCUS       DADTFG010000045        30119 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.045, whole genome shotgun sequence.
ACCESSION   DADTFG010000045 DADTFG010000000
VERSION     DADTFG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.045"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            145..1664
                     /locus_tag="J1138_001884"
                     /pseudo
     CDS             145..1664
                     /locus_tag="J1138_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1722..2057)
                     /locus_tag="J1138_001885"
     CDS             complement(1722..2057)
                     /locus_tag="J1138_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBA8453690.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(2061..2345)
                     /locus_tag="J1138_001886"
     CDS             complement(2061..2345)
                     /locus_tag="J1138_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBA8453691.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            complement(2407..2580)
                     /locus_tag="J1138_001887"
     CDS             complement(2407..2580)
                     /locus_tag="J1138_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453692.1"
                     /translation="MSYFSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDN"
     gene            2681..2866
                     /locus_tag="J1138_001888"
     CDS             2681..2866
                     /locus_tag="J1138_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453693.1"
                     /translation="MSRRYSLATITLVILFCGRPHCRKISVRQYYQKRVIFYPTTLQR
                     TAFKKYLFLIRDIIISS"
     gene            2827..2946
                     /locus_tag="J1138_001889"
     CDS             2827..2946
                     /locus_tag="J1138_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBA8453694.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            2981..3324
                     /locus_tag="J1138_001890"
                     /pseudo
     CDS             2981..3324
                     /locus_tag="J1138_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3697..3963)
                     /gene="minE"
                     /locus_tag="J1138_001891"
     CDS             complement(3697..3963)
                     /gene="minE"
                     /locus_tag="J1138_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBA8453695.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3967..4779)
                     /gene="minD"
                     /locus_tag="J1138_001892"
     CDS             complement(3967..4779)
                     /gene="minD"
                     /locus_tag="J1138_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBA8453696.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(4803..5498)
                     /gene="minC"
                     /locus_tag="J1138_001893"
     CDS             complement(4803..5498)
                     /gene="minC"
                     /locus_tag="J1138_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBA8453697.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTSVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            6018..6386
                     /locus_tag="J1138_001894"
     CDS             6018..6386
                     /locus_tag="J1138_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453698.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(6489..6890)
                     /locus_tag="J1138_001895"
     CDS             complement(6489..6890)
                     /locus_tag="J1138_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HBA8453699.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            7132..7425
                     /locus_tag="J1138_001896"
     CDS             7132..7425
                     /locus_tag="J1138_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453700.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDINK"
     gene            7497..8156
                     /locus_tag="J1138_001897"
     CDS             7497..8156
                     /locus_tag="J1138_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBA8453701.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            8233..8694
                     /locus_tag="J1138_001898"
     CDS             8233..8694
                     /locus_tag="J1138_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBA8453702.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKGLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(8901..9222)
                     /locus_tag="J1138_001899"
                     /pseudo
     CDS             complement(8901..9222)
                     /locus_tag="J1138_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
     gene            9488..9907
                     /gene="umuD"
                     /locus_tag="J1138_001900"
     CDS             9488..9907
                     /gene="umuD"
                     /locus_tag="J1138_001900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBA8453703.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPIIISSEDTLDVFGVVIHVVKAMR"
     gene            9907..11175
                     /locus_tag="J1138_001901"
     CDS             9907..11175
                     /locus_tag="J1138_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBA8453704.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANYAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGSQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(11220..11750)
                     /gene="dsbB"
                     /locus_tag="J1138_001902"
     CDS             complement(11220..11750)
                     /gene="dsbB"
                     /locus_tag="J1138_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBA8453705.1"
                     /translation="MLRFLNQCSHGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(11896..13437)
                     /gene="nhaB"
                     /locus_tag="J1138_001903"
     CDS             complement(11896..13437)
                     /gene="nhaB"
                     /locus_tag="J1138_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBA8453706.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            13659..14378
                     /gene="fadR"
                     /locus_tag="J1138_001904"
     CDS             13659..14378
                     /gene="fadR"
                     /locus_tag="J1138_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBA8453707.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(14541..16073)
                     /locus_tag="J1138_001905"
     CDS             complement(14541..16073)
                     /locus_tag="J1138_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBA8453708.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            16403..17701
                     /locus_tag="J1138_001906"
     CDS             16403..17701
                     /locus_tag="J1138_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBA8453709.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPLRPGHLHGAHS"
     gene            17711..18781
                     /gene="dadX"
                     /locus_tag="J1138_001907"
     CDS             17711..18781
                     /gene="dadX"
                     /locus_tag="J1138_001907"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBA8453710.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPRARVWSVVKANAYGHGIE
                     RIWSAIGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(18838..20574)
                     /locus_tag="J1138_001908"
     CDS             complement(18838..20574)
                     /locus_tag="J1138_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBA8453711.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PTGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(20669..21583)
                     /gene="ldcA"
                     /locus_tag="J1138_001909"
     CDS             complement(20669..21583)
                     /gene="ldcA"
                     /locus_tag="J1138_001909"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBA8453712.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLSVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            21683..22294
                     /gene="emtA"
                     /locus_tag="J1138_001910"
     CDS             21683..22294
                     /gene="emtA"
                     /locus_tag="J1138_001910"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBA8453713.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(22296..23030)
                     /locus_tag="J1138_001911"
     CDS             complement(22296..23030)
                     /locus_tag="J1138_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBA8453714.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            23231..23485
                     /locus_tag="J1138_001912"
     CDS             23231..23485
                     /locus_tag="J1138_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA8453715.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(23535..25505)
                     /locus_tag="J1138_001913"
     CDS             complement(23535..25505)
                     /locus_tag="J1138_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA8453716.1"
                     /translation="MRLKKRYLCTVLSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPIYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(25531..26385)
                     /locus_tag="J1138_001914"
     CDS             complement(25531..26385)
                     /locus_tag="J1138_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBA8453717.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(26382..27194)
                     /locus_tag="J1138_001915"
     CDS             complement(26382..27194)
                     /locus_tag="J1138_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA8453718.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            complement(27204..27962)
                     /locus_tag="J1138_001916"
     CDS             complement(27204..27962)
                     /locus_tag="J1138_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8453719.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIDAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(27959..28939)
                     /locus_tag="J1138_001917"
     CDS             complement(27959..28939)
                     /locus_tag="J1138_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA8453720.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWAGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(28939..29961)
                     /locus_tag="J1138_001918"
     CDS             complement(28939..29961)
                     /locus_tag="J1138_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBA8453721.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTETAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         7674 a         7248 c         7662 g         7535 t
ORIGIN      
        1 atttatcatt taaatattgt cgctgtttat ctatttaaat ttcttgtgct tttgttttta
       61 tctttgcctt ccgttttggt aatctgaact cataggttat ttgaccagga atgtttgtta
      121 atccaatgaa tggatggtta atctatgaaa ctgaaaaaac tccccggatt tagtttagga
      181 cttattgctc tggcggtggg taatgcatat gtaacacaat tgttggatat ttatagtata
      241 atttcctata taaccgatga agaatcaccg attgaaatca aaaataatac ttctacaagt
      301 aatggggagt atctaatcac ttaagacgaa agccatgctg tgaaagtgga tgacggtgta
      361 actggatata tacataatgc cagtgtgatg actagtggtg atggatctta tggtatttct
      421 gttgatagcc aaaacaaagt attatatata agcgatagca atattaagac gctctgaaag
      481 cgtatctgac aaagaaaatg gagggataac agccagcgca gtagtcagtg aatttggtgg
      541 caccatcgtt atgaatggtg ataattcagt cgagacgagg gggcatattc agcgggactt
      601 ttaagtcaga ttaatgattc tgggactgtg gaaaataaca ccagtcttga aaccacagat
      661 aaaacgaaca ttgttaccta tggggaaaaa tgcagtagga gttcttgcat gttcaagtcc
      721 tggagagtct cgaacatgtg tcgatgctgt agatgatgaa gtttgtgatt ctaacagtta
      781 cgaagttatt agccgtgctg atttaaaaat gaatggtggt tccataacaa ctaatggtat
      841 taatagctat ggtgcttatg ctaatgggaa aaaagcatat attaatttag attatgtggt
      901 gcttgaaact gtggctgatg gaagttatgc agttgctatt cgacaaggta acattgatat
      961 aaaaaagttc tattacaaca aatggcacta aagcccccat tgcaaaaata tacaatggta
     1021 gagagttatt tttttccaat gtcacttcag aatcaacaga aacgcctacc cccgtcctgc
     1081 gacccgaagc tggcagttac gtcgccaacc tggcagccgc taacactctt tttgttatgc
     1141 gtctgaacga tcgtgcaggt gaaacgcgct atatcgatcc tgtaactgaa caggagcgtt
     1201 caagccgatt ttggctacgt caaattggcg ggcataatgc ctggcgtgac agcaacggac
     1261 agttgagaac gacctcggca caggccatct ggatgggcgt cgatcataat gtgcacacgg
     1321 aagccaatgg ctcacgtatt gagaatgacg caaataacaa tatccagacc cgacttggtt
     1381 tccacacctt tattcgtact caggggaaaa acagcggtcc gcacggtgac gactttgaac
     1441 cttttgttga aatgaacggg atccataaca gtaaagattt tgctgtctca ataaacggtg
     1501 tgaaagttga acaagccggg gcgcgtaatc tgggggaaat taaacatggc gtaaatggca
     1561 atctgaatcc tgcggccagc gtctggggta atgtgggcgt gcagctgggt gataatggct
     1621 acaatgacaa cgcaatgatg gtgggcctga aatataagtt ctgatcccgc cattagctaa
     1681 aaaaccgcgt cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca
     1741 cgcatggtgt catcaattgc cgtgatcaac agcatttgcg gatctttagc gcaaacctga
     1801 ttcagttttt ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg
     1861 ctggcaacgc tggcattacc cattaccgca acaatttctg caacctgtgc gctgtcagtt
     1921 tttgccattt cgttggcttc tgcgcaagta atataggttt ctgatggcaa accgttctta
     1981 atattgtagt cctgcgccca ggtcattggt gcgaaaacaa acagacccgc cagtaaagca
     2041 aattttttca tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca
     2101 atcacggcac ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca
     2161 ccaacggcgg cacccacttt tgcaccttta cgcgcatttt taccgtcgcg gcctttttct
     2221 gttactgcac caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg
     2281 ccagtaagta aaccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg attcatatcc
     2341 gccatcgctg gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc
     2401 ttcaacttag ttgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa
     2461 gatctgctta actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtgactgt
     2521 gatgatagga attatgtttt ttacgggaat gagaataatc tcaaatgaga aataactcat
     2581 tgattgacaa tatttttatt caagaagtgt cattgactgt taacgcaatg ttgtaaaggt
     2641 aagataatct gatttatcaa tattattgtg tgattgttat gtgagcagaa gatattcatt
     2701 agcaacgatt acattagtca ttttattttg tggacggcct cattgtcgaa aaataagcgt
     2761 acgacagtat tatcagaaaa gagtgatttt ttatccaaca acacttcagc gcactgcgtt
     2821 taaaaaatac ctctttctta tacgggatat catcatttca tcatgatgtc tttgatgagc
     2881 ggtgaacaca atacatttgc gctgtctctt caggatgaat cccctggtct ggttcctgcg
     2941 ggctgatgtt gcagcagagc taaggtcact tagacgctac tatcatttat ccaatgcatg
     3001 gaatcgaaat cagtcgatac ccgcagtata tatcgtgaac tgggcgcaac gctgagttac
     3061 aacatgcgtc tggggaacgg tatggaaatt gaaccctggc tgaaggcggc tgtgcgcaaa
     3121 gaatttgtcg atgataatcg agtgaaagtg aatagtgacg gtaattttat caatgatttg
     3181 tcgggtagac gtggaatata ccaggcaggt attaaagcct cattcagcag tacgttaagc
     3241 gggcatctcg gggtggggta tagccgtggt gccggtgtgg agtccccgtg gaatgcggtg
     3301 gtcggtgtga actggtcgtt ctgaccatca acgattaaac tgcgtttcgg cgctgttttt
     3361 gtttaccggg tgctaaaagg gaaaattctg gggcaaaaaa aagcccgtca gttacggcgg
     3421 gaaacctcat cctatgggag aacaatgaat aatgaaattg cggggttatc atctcccagg
     3481 atatccacgc taacaataag gttatttact caaccaggcg taaacatttt gttctgtgcg
     3541 tgggaacagc cttaaggtgt aaagggggag ggggaaatag caatgaatag tatcagcaag
     3601 aatactcgcc gctttaccac aacgtggatg agagagatga aaaactcaag gcagagatga
     3661 ctctgccttg aagataaatg cgcttttaca gggggcttat ttcagctctt ctgcttccgg
     3721 taaggtcacg ttcagctcaa gaatagaaat atcgccatct ttttgctcaa gctgtacggt
     3781 taccatctca ggatcaattt gtacatattt acaaatgacc tcaagaatat ctttacgcaa
     3841 ctgcggcaga taatgcggtt ctgcatcgct gcgacggcgt tcagcaacaa taatctgcaa
     3901 ccgttctttt gcaatgttgg ctgtgttttt cttccgcgag agaaagaaat cgagtaatgc
     3961 cataacttat cctccgaaca agcgtttgag gaagcctttc ttctcttctt caatgaagcg
     4021 gaaaggacgt tcttctccca acagacgttc tacggtatct gcgtaggctt tacccgcatc
     4081 ggcgttaatg tcgagaatga ccggttcacc ctggttagag gcgcgcaata ctgattgatc
     4141 ctctgggatc acgccgacga gtttgatgcg caggatctcc agcacatctt ccatgctcag
     4201 catgtcacct ctgcttacgc ggcctgggtt atagcgcgtt aacagcaggt gctctttaat
     4261 cggctcttcg ccattttctg cgcggcgtga tttcgacgcc agaatgccta aaatacggtc
     4321 agagtcgcgt actgaggaga cttccggatt ggtggtaata atggcttcgt ctgcaaaata
     4381 gagtgccatt aacgcaccgg tttcaatccc tgccggggag tcacaaacga taaattcaaa
     4441 atccatcgct ttcagatcat caagaacttt ggcgacccct tcacgggtga gggcatcttt
     4501 atcgcgagtt tgcgatgccg gaagaatata gagattttca gtacgtttat ctttaattaa
     4561 cgcctggttt agcgttgcat cgccctgaat gacgttgacg aaatcgtaaa cgacccggcg
     4621 ttcacaaccc ataatcaggt cgagattacg caggccgata tcaaaatcta tcacgacagt
     4681 tttctttccc ttctgggcca aaccagtggc gatggccgcg ctggaggttg tcttaccaac
     4741 accccctttg cccgaagtaa caacaataat gcgtgccata gaaattcctt gttaaaaagg
     4801 gatcaattta acggttgaac ggtcaaagcg ttttcgacta actgcagtcg tgccgctttg
     4861 ccataaaatt ctgctgggat ttgatcactc agccagtatt cacctgcgat ggacaccagt
     4921 tccgccatca ggttcgtaca aaatatttgc gtttcccggt caccacttgc ccctgccagc
     4981 gcacgaccgc gcatcatgcc atagacatga atgttaccat cggcaatcaa ttcggcccct
     5041 gcgctaacgt ggcttgtaac aatcagatca cattgtggag cataaatacg ctgaccggaa
     5101 cgcactgggg tatcaattaa acgcgttttt gtgaccgacg ttgtattttg cgctggagcc
     5161 tgcggtgcgg gagctggacg cggcgctttt tcctttcctt ccgtcaggat aggcagcccc
     5221 atcttttcaa tttcggcttt aagttgcgca tctttgcagc cacttacgcc aataacccgc
     5281 aaaccagttg ccgaaaccgc cttatgcatc gccgaccagt ttaccgggtc ttccagtgca
     5341 ctgacgttga gtacaacggg ggcatgtttt aaaaatgcgg gggcctgagc gattttgtct
     5401 tccagcgcct gatgaataac cttaggttct gcctcatgca gatgaaccac agataaagtg
     5461 aagctactgc ctttaagctc gattggcgtg tttgacatcc tggccttact caattagcta
     5521 ttaatcatcg ccagcgcgcg atgatgttcc gaagactata aggcatgtta tagtctggat
     5581 tatattgagg caagtcaccc tcccatttat tcagagtaaa agtctattct gtgataaatg
     5641 gcgctgattc atagcttaaa aaataccctt gtcaatcaac ccattgccgt cgtacttttg
     5701 attgttctta tttacgcttc ctttttccgc accctaatcg tgacagcatc ttcaggcctg
     5761 tttaaatatc cagtgttgag gcgagtgtat tttttcacca cgccaggctt tcttaacttt
     5821 ccattttttg cagcatcatt aaagaacaga tacccgttat cttaatatcc gccacagaat
     5881 tgctctatca tagaaaccgc agaagacgtc tgatacttct gtcaggtcat cgtaactcgt
     5941 ccaggtcagc ggttttgatt acggattgat gatgaaatga gcataagtgt tgtacggttg
     6001 gattatcagg atgtgctatg aatatgatgc gtatttttta tatcggattg tcaggtgtgg
     6061 ggatgatgtt ctcatcaatg gcgtctggaa atgacgctgg cggacttcaa tctccggcgt
     6121 gcggcgttgt ttgcgacccg tatatttgtg ttaactcaga tggcatttcc ccagagttaa
     6181 caaggaaata tctcggcgaa aaagccgctg aaaacttaca atcagtacaa ggttacgatc
     6241 ccagcgaatt tacattctct aacggtgtat tttgcgatgt taaagaaaaa ttatgtcgtg
     6301 atgatcgtta ttttggtgtg aatggtaagc ggagtggaaa aatcaatcaa accaccacaa
     6361 agatgttatt tatgtgtcgt gagtgatgtc ttatttacat tatgttctat tcgtcattaa
     6421 cctcttaagc gacagagagg agtggggcgt taacgtttat gacggggaga gtccccgtcg
     6481 gttgatattt acttaatttg aatatcgacg ttatattttt tagttttgtt tttacgggcg
     6541 tcgtttcggg tttgcagcac tcgcagctca tatttgccag aagcagggag tgagtattgt
     6601 ccgtgactat cgagttctgg ggaatatctg gataaatcaa cagaatcatc gattcctggc
     6661 ccgaagaggt aagtatcagc gccttcgtta gagatactca cgtgcacttt ctgacctttt
     6721 ttggcataga aggtatatgt atcgtaatcg tatcccttta tttcgccgga gtactgagcg
     6781 ctgctatggc cttttctgaa ctcaacattg acattcttac ccgcagcaaa gctcgtggag
     6841 gttagcagtg caagcaacaa tacagcctta ctgatgccct taattttcat tggtagtctc
     6901 ctgttcagaa tgagtacgat gaactgttat aatataacaa tccttcacgg gagaattcca
     6961 gtggctttcc tgtgttcggt taaatcccgg cgcggcattt ttgccggggg gattacttat
     7021 aaagggggca tggactgaac aaaccgtaga aatctacagc tggctggctt gtggcgcggg
     7081 tttcattgat gttagcttat gccaaaacct ggcatcttaa agagtaaaag tatgttttgt
     7141 gtgatttatc gaagcagcaa gcgtgaccag acctatttat atgtcgaaaa aaaagacgat
     7201 ttttcgcgtg ttcctgagga actgatgaaa ggttttggtc agcctcagtt agcgatgatt
     7261 cttccgctgg atgggcggaa gaaactggtt aatgccgata ttgaaaaggt taaacaggca
     7321 ttaaccgagc aaggttacta tttgcagtta ccgccaccac ccgaagattt gctgaagcaa
     7381 catctttccg tcatggggca aaaaacagac gacattaaca aataaccgat atccggcggt
     7441 ggcattatct ttgtcggcgc gggttttcat atccacgata aggtgagggg aacgttatgt
     7501 atcaacatca caactggcaa ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg
     7561 ttggcagtaa ctatgccaaa catattaaag agatgggcag cgcagtaccc gaagagccag
     7621 tgctgtttat taaaccagaa acggcactgt gcgatctgcg gcagccattg gcgatcccat
     7681 ccgatttcgg ttcagttcat catgaagtcg aactggcggt gttgattggc gcgacgctgc
     7741 gtcaggctac ggaagagcat gtccgcaaag ccattgccgg ttacggcgtg gcgctcgatc
     7801 tgacattgcg tgatgttcag ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcca
     7861 aagcgtttga taactcttgt ccgctttccg ggtttattcc cgcggcggag tttaccggcg
     7921 acccgcaaaa tacaacgctg ggcctgagcg taaacggcga gcaacgccag caaggtacga
     7981 ctgcggacat gatccataaa atcgttccgc tgatcgctta tatgagcaag ttttttaccc
     8041 tcaaggccgg tgacgttgtg ctgacaggca cgcctgatgg tgtcggcccg ttgcaaagcg
     8101 gtgatgagct gacagtcact ttcgatgggc attctttgac aactcgcgtt ttgtaatact
     8161 ttttgccgcc tgaaagcggc ggcaaaactt gcatcgctat gccagactgg ttataaggtg
     8221 cgttttaacg taatggcgga acacctgatg agcgatgtac ctttctggca aagtaaaacc
     8281 ctggacgaaa tgagcgatgc ggaatgggag tcgttgtgtg atggttgcgg tcagtgttgc
     8341 ctgcataaac tgatggatga agacaccgac gaaatctact tcactaacgt cgcctgtcgc
     8401 cagctcaata ttaaaacctg tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc
     8461 gactgcatta aattaacccg tgaaaatctg ccaacattcg aatggctgcc aatgacctgc
     8521 gcttatcgtt tgctggcgga aggtaaaggt ttacctgcgt ggcatccgct acttactggt
     8581 tcgaaagcgg caatgcatgg tgaacgtatc tctgtgcgtc atatcgcagt aaaagaatca
     8641 gaagtgattg actggcagga tcatatctta aataaacctg actgggcgca gtgataaatt
     8701 taatagatgt tgatgggggc tttcttaatt cacgtctgga tgctcgccat ttcgtagccc
     8761 tgtttttgaa agcgctctgt cataatgaga gttacccgat accttattat caacaggtaa
     8821 ctctcaatct ttaaaaaacg tctgtatttt taaatgtgat aaactttata ccttgagtac
     8881 atagagagaa taatcgctta tcagacttca ggtatctcaa agagtgtctt cttaccgtgt
     8941 cttttctgat actcattaca ggtgttaatc atttttttgg ccgcttactt ttagtaaaga
     9001 aagcattaaa tcatcataat caacgtagaa tctggttgtt tcagtttccg tttttatcac
     9061 cccgatggcg gctatttcgg tagttaattt caatttggcg gcatcgatat ctttattcgc
     9121 ttgtttaacc gtgttagaca gggtggtaaa gaaactctgc acagatttta acttgttctt
     9181 caattctgga atcagtttcc cttcaactac gcccgcagca atagaacaca gtttcttgat
     9241 gccgtcatcc agtaccttaa tgagaatgcc tttctgggcg aatgcttcga ttctatttta
     9301 aaccaggtga tatttttcct ttctgctgga tggacgctgt cgccagaagg ccgcgtgagc
     9361 acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga tagcctgaat
     9421 cagtattgat ctgctgacaa gaacagacta ctgtatataa aaacagtata gcttcaggca
     9481 gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt ttccgctatt
     9541 tagcgatctt gttcagtgtg gctttccttc accggccgca gattacgttg aacagcgcat
     9601 cgatctgaat caactgttga tccagcatcc cagcgcgact tatttcgtca aagcaagtgg
     9661 tgattctatg attgatggtg ggattagtga cggtgattta ctgattgtcg atagcgctat
     9721 taccgccagc catggtgata ttgtcatcgc tgctgttgac ggtgagttta cggtgaaaaa
     9781 attgcaacta cgcccgacgg tacagcttat tcctatgaac agtgcgtact cgcccattat
     9841 catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg ttaaggcgat
     9901 gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg ccagctgtga gacggtgttt
     9961 cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg ttgcgttatc
    10021 gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg gcgatccctg gttcaaacaa
    10081 aaagatctgt ttcgtcgctg tggcgtggtt tgctttagca gcaattatga gctttacgca
    10141 gacatgagta atcgggtgat gtcgacgctg gaagaactat cgccccgcgt cgagatttac
    10201 agtattgatg aggcattctg cgatctgaca ggtgtgcata attgtcgcga tctgactgat
    10261 tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt tggtgtgggg
    10321 atcgcccaga ccaaaacgct ggctaagctt gccaattatg cggcaaaaaa atggcagcgg
    10381 cagacgggcg gggtggtgga tttatcaaat ctggaacgcc agcgtaaatt aatgtctgct
    10441 ctccccgtgg atgaagtctg ggggattgga cggcggatca gcaaaaaact ggacgcgatg
    10501 gggatcaaaa ccgttctcga tttggcggat tcagatatcc ggtttatccg taaacatttt
    10561 aatgtcgtgc tcgaaagaac ggtgcgtgaa ctgcgcggtg aaccctgttt gcaactggaa
    10621 gagtttgcac cgacgaagca ggaaattatc tgttcccgct cgtttggtga acgcatcacg
    10681 gattatccgt cgatgcggca ggccatttgt agttacgctg cccgggcggc ggaaaaactt
    10741 cgcagcgagc atcaatattg tcggtttatc tccacgttta ttaagacgtc accatttgcg
    10801 ttcaatgaac cttattacgg caatagcgca tcggtaaaac tgctgacgcc cactcaggac
    10861 agcagggata tcattaacgc cgctacgcga tctctggatg ccatctggca agcgggccat
    10921 cgctaccaaa aagcgggcgt gatgctgggg gatttcttca gtcagggagt cgcgcagctc
    10981 aatttattcg atgacaacgc accgcgcccc gggagtgagc aattgatggc ggtaatggat
    11041 acgctgaatg ctaaagaggg cagaggaaca ctctattttg ccgggcaggg gagccagcaa
    11101 caatggcaga tgaagcgtgc catgctttca ccatgttata caacgcgaag ttctgattta
    11161 ctgcgggtca aataaacata gcggcaggaa aaagcgctcc cgcaggagcg ccgaatggat
    11221 tagcgaccga acaggtcacg ttttttcgct ttaaacggct gggaaatcac caccagcacc
    11281 gcgacaatca ggtaagcgat aaaaataccg agcagccact gcggcatttc cagacctaaa
    11341 aattcccact gacgctcggc gcaatcgcca gaggcgacaa acacttgcgg cacccattta
    11401 tccagcggca gccattccgg gaaacgagcc ataaaatcac aggtcgcaaa cggcgaagga
    11461 tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac cgcggaacgc actatacaac
    11521 cagataacca tcgctacata acgcagcgga gttttcgggg cgatcgcgcc aatcagcgca
    11581 gcacccagaa cgccgaataa cgcgcagcgt tcataaatac agagcacgca aggtttcagt
    11641 aacatcacat gctggaacca cagcgccgtc agttccagtg ccagagcagt aaacgccatc
    11701 aacagccatg cgccccggcc atgtgaacat tggttcaaaa atcgcaacat aatcatttcc
    11761 ctgcaatatg catagagtgc gcagtttaaa ccaattcatt cgctgcgcca ccaggaggca
    11821 gcaaaaatag gacgcaattg gacaattatc gggcgaaaag gcaaaccggg catttatatg
    11881 cccggtaagt tgttatcaaa gcgttgctat ccagcccatt tgcataaacc attcggttac
    11941 aggagcaagc gtaaactcga cgcagagcag cccgacgagt gtcaggacga gggtgtaagg
    12001 cagggccatc cacaccatgc ggccataaga gagacgaatc aatggcgcga gtgcagaggt
    12061 cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg acggagggca gattggtacc
    12121 ggtattaatg gcgaccgcca gcagctcata ttgcttcaac gtgatagcgc cactttccat
    12181 tgccgctttc gcttcgttga tataaatcgt ccccacgaag acgttatccg aaatggatga
    12241 cagcagaccg ttgaaaatat agaacaacga cagctgagca tgttcagatg cctgcaacac
    12301 aaactgaata atcggcgaaa acagttgttg gtcgataatc accgcgacta ctgagaaaaa
    12361 cactgtcaac agtgcggtga atggcagaga ttcggtgaag gctttaccga tagcatgttc
    12421 atcggtaaca ccggtcaatg atgtcgccag aataatgact gacaaaccaa tcaaaccaac
    12481 ttccgccaga tgcagtgcca gcgcagtcac cagccagacg ccgataatcg cctggacaat
    12541 cagacggatt ttatcctgac gggtacgctg gttgcggctt tgatcgtcaa actgttgcag
    12601 tacttcgcgg actttctccg gcagcgtttc accgtagcca aaccaacgca gcttctctac
    12661 cagcaggcag gttaacaggc cacaaatcag aaccgggacg gtcaccggcg acatgcgcag
    12721 gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg atcaggtttt gtggttcgcc
    12781 caccatggtc atcacgccgc ctaatgcggt gccgacaccg gcatgcatca tcagactacg
    12841 cagaaagcca cggaactgtt ccagaaccac tttgtaatgc ttgtcgatat gactatcgtc
    12901 ttgcaggtcg gtatcttcag tacgggaaga ggctacgcgg tgataaatac cataaaaacc
    12961 gactgcaacg ctgatcacca ccgccacgac ggttaaggca tcgaggaacg cggagaggaa
    13021 cgccgccgcc acgcaaaaag agagcgacag cagcattttg gagcgaatac ttaacagcaa
    13081 acgggtaaat atgaacaaca acagctgttt cataaaatag atacccgcca ccataaacat
    13141 cagtaacagc aagacttcaa gatttgccgc cacttcttca cgaacatgtt ccgcgctggt
    13201 catgccgatg aataccgctt cgatagccaa cagaccaccg gggagcagcg ggtagcattt
    13261 cagggccatc gccagagtga aaataaattc cgctaccagc aaccagccag cgacgaaagg
    13321 gctgatgagg aaaattaacg gatttacgat taagaaaatg atgagggcga gtttgtacca
    13381 gtcgggggac tggcccaaaa aattgcgcca tagtgcgcgg ccccaggaga tctccatgat
    13441 ggtttccctt accttagaaa taatcaatga tgtttttatg tttaaacgca aagcttaacg
    13501 gtcagacagg agtgaggcaa gtcttgatag tcaaggggaa agagatgcag aaaatgaagc
    13561 cttgatccct tttttcttct ttttgtcttc tatcagcgta gttagccctc tggtatgatg
    13621 agtccaactt tgttttgctg tgttatggaa atctcactat ggtcattaag gcgcaaagcc
    13681 cggcgggttt cgcggaagag tacattattg aaagtatctg gaataaccgc ttccctcccg
    13741 ggactatttt gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg cgtactacgt
    13801 tacgtgaagt gttacaacgt ctggcacgag atggctggtt gaccattcaa cacggcaagc
    13861 cgacgaaggt gaataatttc tgggaaacct ctggtttaaa tatccttgaa acactggcgc
    13921 gactggacca cgaaagtgtg ccgcaactta ttgataattt gctgtcggtg cgtaccaata
    13981 tttcaactat ttttattcgc accgcgtttc gtcaacatcc cgataaagcg caggaagtgc
    14041 tggcaaccgc taatgaagtg gccgatcacg ccgacgcctt tgccgagctg gattacaaca
    14101 ttttccgcgg cctggcgttt gcttccggca acccgattta cggtctgatc cttaacggga
    14161 tgaaagggct gtatacgcgc attggtcgtc actatttcgc caatccggaa gcgcgcagtc
    14221 tggcgctggg cttctatcac aaactgtcgg cgttgtgcag tgaaggcgca cacgatcagg
    14281 tatatgaaac agtgcgtcgc tatgggcatg agagtggcga gatttggcac cggatgcaga
    14341 aaaatctgcc gggtgattta gccattcagg gacgataatc cttccagacg gatgacaaac
    14401 gtataattgc ctgatgcgct acgcttatca ggcctacgtg tttttttgca atgtattgaa
    14461 tttgcgatac tttgtaggcc ggataaggca tttacgccgc atccggcaaa aatgagtcct
    14521 cctcaaacga ggaggactat ttacagattt cccattctcg gcgggcaacg ttccagcaac
    14581 tcgacgctgc cgtcttcgtt ttgctgttcc agcatcacat caaatcccca caggcgatgc
    14641 acatgcttca gcacttcttt gcgcccccga tccagcggtg cgcgattatg tggaatgtaa
    14701 cgcagcgtca gcgaacggtc gccgcgcaaa tccacgttcc agatctgaat attcggctcc
    14761 agattactta agttatattg cgacgataac cggttacgga tttcccgata accttcttca
    14821 ttatgaatag cggaaatctc cagataatta tgccgatcat catccagcac ggtgaagaag
    14881 cggaaatcac gcatcacttt cggtgacagg aactggctga taaaactctc atctttgaaa
    14941 tcacgcatcg cgaaatgcag cgtttccagc cagtcggaac cggcgatatc cgggaaccag
    15001 tatttgtctt cttccgttgg tgactgacaa atccgtttaa tatcctggaa catggcaaac
    15061 ccgagggcat acgggttgat gccgctgtac cacgggctgt tatagggcgg ctggaagacc
    15121 acattggtgt ggctgtgcaa aaactccagc ataaaacgtt ccgttacttt cccttcatca
    15181 tacagatggt taaggatggt gtagtgccag aaggtcgccc agccttcgtt catcacctga
    15241 gtctgttttt gcggataaaa atactggctc accttacgca caatacgcag gatttcacgc
    15301 tgccatgatt ccagcagtgg cgcatttttc tccataaaat agagcaggtt ttcttgtggt
    15361 tcggacggat agcggcgcgc ttcagcaacc gttttctctt cctcgcgctt cggcagggta
    15421 cgccagagca tattgacctg actttgcaga tactcttcgc gactcttctg ccgggctttc
    15481 tcttcttgca gcgagatttt ttgcgggcgt ttgtaccggt ccacgccgta gttcatcagc
    15541 gcgtggcacg agtccagaag ccgttctact tcatcaacgc cataacgctc ttcgcactcg
    15601 gtaatatatt tacgggcaaa aatcagataa tcgacaatcg aactggcgtc ggtccagcta
    15661 cggaataagt aattgttttt gaagaaagag ttatgcccat agcaggcatg agccatcacc
    15721 agcgcttgca tggtaatggt gttctcttcc atcaggtaag cgatacacgg gttagagtta
    15781 atgacgattt cataggccag tccttgctga ccgtgcttat acagccgttc agtctcgata
    15841 aactttttac cgaatgacca gtgcggatag ttaattggca tgccgacgct ggagtaggca
    15901 tccatcatct gttctgaggt tatcacttca atctggtgcg ggtaggtatc cagccggtag
    15961 agtttcgcca cccggtctat ctctgccaga taaacatcca gcaggtcgaa cgtccagtcg
    16021 ggtccatcgc tcaaacgtgt ggtgtcctta ttcatagaat cgatcgtcgc catacgcgca
    16081 cctcattgtt gtcggcgctc tctgtgtgga gcacctcatt tcaagcatag aacacctgtt
    16141 aaaaaccgcg tcgccggaga atttttttct ttgcgatttc ttattatcag agtgccacta
    16201 atccgcttct gaacggaatt ttatgctgga taaaaagggc gttcagcagg agatactaaa
    16261 gacgccatat tgccgcagag tcagggagat gtgagccagc tcaccataaa aaagctgtat
    16321 gttgaataat attttcaact gagttatcaa gatgtaatta gattattgtt cttttactgt
    16381 atctaccgtt atcggagtgg ctatgcgagt tgtcatactg ggaagtggtg tggtaggcgt
    16441 tgccagcgcc tggtacttaa atcaggcagg acatgaggtc accgtcattg atcgggagcc
    16501 gggggccgcc ctggaaacca gtgccgcgaa tgcagggcaa atctcccccg gatatgctgc
    16561 accgtgggcg gcaccaggtg tgcctttaaa agcgattaaa tggatgttcc agcgccatgc
    16621 gccgctggcg gttcgtctcg acggtacgca gttccagttg aaatggatgt ggcaaatgtt
    16681 acgtaactgc gacaccagcc actatatgga aaacaaaggg cggatggtgc gtctggcgga
    16741 atacagccgt gattgcctga aagcattgcg cgccgaaacc aatattcagt atgaagggcg
    16801 tcagggtggg acgctgcaac tgttccgtac cgaacaacag tatgaaaacg cgacccgcga
    16861 tatcgccgtg ctggaagatg ccggcgtacc gtatcagctg ctggaatcca gccgcctggc
    16921 ggaagtggag cccgcgctgg cagaagtggc gcacaaactg acgggcggcc tgcaactgcc
    16981 caatgatgaa accggagact gccagctgtt tacccagaat ctggcgcgga tggcggagca
    17041 ggcgggggtt aaattccgct ttaatacgcc cgttgaccaa ctgctttgcg acggcgagca
    17101 aatctacggc gtgaagtgtg gtgatgaagt gattaaggcc gatgcgtatg tgatggcgtt
    17161 tggttcttac tcgacggcga tgctcaaagg cattgttgat attccggtgt atccgctgaa
    17221 aggctactcg ctgactattc cgattgcgca ggaagatggt gcgccggtgt ctactatcct
    17281 tgatgaaacc tacaaaatcg ccattacccg tttcgataac cgcattcgtg ttggcggaat
    17341 ggcggaaatt gttggcttta acaccgagct gttgcaaccg cgtcgtgaaa cgctggagat
    17401 ggtggttcgc gatctctatc cacgcggcgg tcatgttgag caggcgactt tctggactgg
    17461 cctgcgcccg atgacgccag acggcacgcc ggttgtcggg cgtacacgct ttaaaaatct
    17521 gtggctgaat accggtcacg gcacgctcgg ctggacgatg gcttgcggtt ccggtcagtt
    17581 gttaagcgat ctgctctcgg ggcgcacgcc agcgatcccg tatgaagatc taagcgtagc
    17641 gcgctacagc cgtggattta cgccattacg cccgggccat ttacatggcg cacacagcta
    17701 aggaaacgag atgacccgtc cgatacaggc cagcctcgat ctgcaggcat taaaacagaa
    17761 tctgtccatt gtccgccagg ccgcgccgcg cgcgcgcgtc tggtcggtgg taaaagcgaa
    17821 cgcttacggg cacggtattg agcgtatctg gagcgcgatc ggggctaccg atggctttgc
    17881 attgcttaac ctggaagagg caataacgtt acgtgagcgc ggctggaaag gaccgatcct
    17941 gatgctggaa ggatttttcc atgctcagga tctggagatt tatgaccagc accgcctgac
    18001 cacctgcgta cacagcaact ggcagctcaa agcactgcaa aatgcgcggc taaaagcacc
    18061 gttggatatt tatcttaaag tgaacagtgg aatgaatcgg ttgggcttcc agcccgatcg
    18121 cgtgcttacc gtctggcagc agttgcgggc gatggcgaat gttggcgaaa tgaccctgat
    18181 gtcacatttt gccgaagcgg aacatcctga tggaatttcc ggcgcgatgg cgcgtattga
    18241 gcaggcggcg gaagggctgg agtgtcggcg ttcgttgtcc aattcggcgg cgactctgtg
    18301 gcacccggaa gcgcattttg actgggttcg gcctggcatt attttgtatg gcgcttcgcc
    18361 gtccggtcag tggcgtgata tcgccaatac cggattacgt ccggtgatga cgctaagcag
    18421 tgagattatt ggtgtccaga cgctaaaagc gggcgagcgt gtgggctacg gcggtcgcta
    18481 tactgcgcgc gatgaacagc gaatcggcat tgtcgccgca ggatacgccg acggttatcc
    18541 gcgccacgcg cctaccggta cccctgtttt agtggacggc gttcgcacca tgacggtggg
    18601 gactgtctcg atggatatgc tggcggtcga tttaacgcct tgcccgcaag cgggtattgg
    18661 tacgccagtt gagctgtggg gcaaggagat caaaattgat gatgtcgccg ccgctgccgg
    18721 aacggtaggc tatgagttga tgtgcgcgct ggcgttacgc gtcccggttg tgacggtgta
    18781 acttgttatc gctggatgcg accagcgtcg tatccagcgt tacaaaacac attaaactta
    18841 agattcggct tcttcctccg ctacccgaac accaatcttc aacacttcat tatcttcttt
    18901 ctcggccacc gtccagatca tcccggcaaa ctctacctgg tcaccgacaa ccggtgctgc
    18961 gcctaacaac tgctggacga tttcacccag cgtttgctgc ttatcgcgat attctcgccc
    19021 gtcttccagg ccatatatca gcgccacatc agcatatttg gcgctggctt cgagaatgaa
    19081 gtcaccaaag aagcgttgat ccagcgcgac cggcggcgat tggctgaaca gtttaccgag
    19141 cgccgggaga tcgcgttccc gaccaattac acacaacaca tcgccttcgc gcagtcgggt
    19201 gctgccggtg ggatgaagca actggttatc acgaaacagt gccgcaatac gcgtctcttt
    19261 tggcatatgc aaatcacgca gtgccgcgcc cacgcaccat ttatcggcac tcaattgata
    19321 aacaaactgc tcccacggat tttccggatg aatatccagg ccaacgcgtg acaccggacg
    19381 tcccactggc ggaacgacca ctttggcttt tttcgccgcc cacgagagtg atgttccctg
    19441 caatagcagt gaaaccagga ccacaaagaa ggcgacatta aagaacagtc gcgcattctc
    19501 cagccccgcc atcatcggga acacagccag aataatcggc accgcgccgc gtaatcctac
    19561 ccagctgata aacacgcgct cacgcagatt gaagccacgg aacggtagca atccggcaaa
    19621 taccgaaaga ggacgggcga agaaaatcat ccatgcggac aaaatgagcg ccggaatggc
    19681 aataggcagc agatcgcttg ggttaaccaa tagccccagc accaggaaca tggcgatttg
    19741 cgccagccag gcgaggccgt cgaaattttg caggatgccg tagcggttgc gaatcggccg
    19801 attacccagc agaaaaccgc acagataaac agccagaata ccgctgcctt ccagcgccgt
    19861 ggttaaagca aaaattaaaa taccgccgct taatgccagc aatggatata atccggtggg
    19921 cagggcgatg cgattaatca tttgcagcag taaataacca ccgccaagcc cgatgacaat
    19981 cccgaggcca aattgctgca gaatatcgac cacgaacatc caactgacac tgctttcatg
    20041 ttgctggatc atcgcaatca gggtaatcgt cagaaagacc gccattggat cattactgcc
    20101 ggattcaatt tccagcgtcg agccaacacg ttcgttaagc cccttaccac ccagcagaga
    20161 aaagaccgct gcagcatcgg tggagccgac gatagcgccg attaataatc cttcaatcaa
    20221 atcaagatta aacagccacg ccgccatcat gccggttaaa ccagaggtga taagcacgcc
    20281 cagcgtcgcc agcgacagtg ccggtcctaa cgccacacga aaggaactgg cctgagtacg
    20341 catcccgccg tcgagcaaaa taatcgccag cgccaggtta cttaccatgt aggcgaaggg
    20401 gtaattatca aacgggatac cgccgacgcc atcaaccccc gccagcatgc cgatcgccag
    20461 aaaaataacc agaatgggaa tgccaagacg ggaagaaaat gaactaagta atatactgct
    20521 ggtgacaagg atggatccta agatgaaaag gctaattatt gttgtggcat ccaacggtcg
    20581 gttactcctg attacgctgt ctcttatata aaccctacca tattagcggc agagacagcg
    20641 ttttacttag ccctgaagcg tgattttttt acattttaag aacaggatga ccgctaatcg
    20701 tcaactgggt gccttcctgg gtgttattaa gaatggcatg tgcgcccaac ggcagtgtaa
    20761 ccgtgcgttg ttcatgacca aaatcgaggc cggtaatgag tggaatcgac aggcgggaac
    20821 gcaaaaacgc gtacactgac tccaggttgt aacctgcgtc ataatcatta ggcgtgctgc
    20881 cgctaaagct accgagaata atcgccttct gacgtggcaa aattccagca tgatagagct
    20941 gcaacagcat acgttcgacg cggaaagggt gctcgttaat atcttccagc accagaatac
    21001 cgttctcaat ttttggcatc cacggtgtac caatcagtga aatcagcatc gcaagattgc
    21061 ctccccacag cgtgccttcg gtctgacatg tcgggccttc cccttgccat tcaatggtga
    21121 acgttttatt gcgtaacgcc agccagaagt ggtgctcggt aaaggcattt agttcatccg
    21181 cgccaaagtt tgctaccagc atggggccgc taaaggtgat gacattgcct tgcgccagaa
    21241 gaccgcactg aatggcggta aaatcgctat gtccgcaaat gagtaacggg tcatgttgtt
    21301 ggcgagccac cagtgcctgc cagtcaatat ctgccagtaa acgactggca ccgtaaccgc
    21361 cgcgtacaga cagcacgatg gtgttggggg tagtcagtct ggcaagagaa ttgagatctt
    21421 ccagacgctc cgtttccgta cccgcaaaac gctcacaacg acgggcaata acctcgacgt
    21481 tattgacctg atgccccgcg tcggtaaggc gctggatacc acgcagcgcg gcgtgctgtt
    21541 taatgcagta acccgatggg gcaattaagt gaaacagaga catggcaatt ccttgctgac
    21601 aacagaaacg aaatgtatat catgccgctt aggtgtgccg ttgtcacctc aacggcgatt
    21661 ccaggctata aggacagaag aagtgaaatt gagatggttt gcctttttga ttgtgttatt
    21721 agcgggttgt tcatcaaagc atgactatac gaacccgccg tggaacgcga aagttccggt
    21781 gcaacgtgcg atgcagtgga tgccaataag ccagaaagcc ggtgcagcct ggggcgtcga
    21841 tccacaattg atcacggcga ttatcgctat cgaatcgggt ggtaatccga acgcggtgag
    21901 taaatcgaat gccattggtt tgatgcagat aaaagcttca acctccggac gtgatgtata
    21961 tcgtcgtatg ggctggagcg gtgagccgac gaccagcgag ctgaaaaatc cggagcgtaa
    22021 tatttcaatg ggggcggctt acctgaatat tctggaaacc ggcccgctgg caggcattga
    22081 agatccgaag gtactgcaat atgcgctggt ggtgtcatac gctaacgggg caggtgcgct
    22141 gctacggact ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg atctggatgc
    22201 tgatgagttc ctcgatcacg tagcgcgaaa tcatcctgcg ccgcaggctc cgcgctatat
    22261 ctacaaactt gagcaggcac tggacgcgat gtaaatcagt cgcgcacatt gtccgctttt
    22321 ttccgggctt ctcgctcgag agagaaaata atccgctgta attgccgctc caccgtcggg
    22381 ctgacgttaa gaaaacggaa gctcagacgg ggagtggtga tggtttcatt tttgccatca
    22441 atcactttgc gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac accccattgc
    22501 cccatgttga cttcaatctg agcgaagcgc atgccttcgt gtaattcggc aggctttgct
    22561 gtttccagta atgcgcccat gccgcctaac gacaaatcat acaggcggaa acgtaacgta
    22621 ctgttatccg ccagtttggt ctggcaaaaa taaggcggat ggagtggggc ggtgatgcgg
    22681 aaatatcggc gtcgttgtac aaaccataag gtgggaggcg gtacggtaat aaatgccgga
    22741 agctgcaagt attcactctg ctgtagttgt tcaacagtaa actcgacttt cgcaccctga
    22801 gtttcggcgg taatggtaat gtgctgcgcc tttagcacgg cgatgttgtc ttcggcctgg
    22861 ctgccgaaat ccagcaccag tttatccgga gttattgcca gtattttgct gatcagctgc
    22921 ccgccattcc agctgatacg caaaggaatt gcggctttgt gcaaatcgcg taacacgccc
    22981 aggacggcta acggattttg tttcaggaac tgctcatggt aatgactcac gcggaaaaac
    23041 tcctgactga caaactgtct ttggtttatc ggtcgcagtt aacaaaactt aatacaaatg
    23101 cgcgaatatt ttttacatgt tgttcttaaa tcagccgaca tacgcccagc atcgatcccg
    23161 cccttgccta tacttagagc gttgacgtaa gcatttcttg cgtccgattc atcgaaacga
    23221 gggcctgaac atgggaatta ttgcctggat tatttttggc ctgatagccg gtattatcgc
    23281 caagctaatc atgccggggc gtgatggtgg tggatttttc ctgacctgta ttcttgggat
    23341 agtcggtgcg gtggtcggcg gctggctggc gaccatgttt ggcattggcg gctccatcag
    23401 cggttttaat ttgcacagct tcctggtggc ggtggtgggc gctatcctgg tgctgggcgt
    23461 attccgcctc ctgcgaagag aataagattt tcataaggcg gatagcgata cagatgccgc
    23521 tatccgcttt cacatcagaa cgtatactcg ataccagccc agaaattgcg accttcttca
    23581 ataaaccctt cactgtaggc atatttggta tcaaacaggt tattgaccga cgcattaacg
    23641 ctgaagccat gacctaaggt gtaatcggct cgaatgtggg tcaccgcaaa accggcggct
    23701 ttttgtgaac cgtcactgtt gctgtagctg gaggaacgcg cctcttccga cagcgttacg
    23761 cttaacggct cccacggttt gagagtcatc catgcggtca ttgtctgcgt tggcagatcg
    23821 gttatcttgc cgatgtcttt acgtttggca tcagcgtgga tcagggcgta gctcaatcct
    23881 acatccagta tattgctgat tttcccctta ataccggcat ccagaccgct gtaatccacc
    23941 gtgccgctgt tctgattttg aatggtatcg gcatcgatat tgtgcgagag gatggcatca
    24001 ctcacccggt tatagtaaac gctgacctca aatccccagt cgtgcgtgaa ggcaccattc
    24061 caggttaaat ccaccccgcg cgcgcgttcc ggcttgagct gcgggttaac aatcgctatc
    24121 tggttatacg caggtttgga cgtggtatag cgttctttca gcgtcggaaa gcgtgtgcgg
    24181 tcatagtacg aaagcgccag cgtgtcttca ttggcaaagt ggtatttccc catcacctgc
    24241 cagttaaaag ctgactgatt gttgtcgtca taatgggtga tactgccatc tttctcatgt
    24301 tttttcgctt ctacgctatc gcgccagtca tagctgattc cagccacgac atcgacatta
    24361 tcggcagcag cccattgata ttcactggcg agcgaccagg tacgatcttc atagcgatcg
    24421 taagcggcgt gcggcgcacc tttttcccgg tgtacgtcat ctttccagtt aacggcaaac
    24481 gacagcagat cgttttcacg cacatcggct gccagttgta acccggcacc gtcgctgtaa
    24541 tcggaataat ggctgtagct gcctttttta tttttcaaat cagccagcga gttgtacatc
    24601 attagcgtat tttcaaaggt gtcgcgatac agccgacttt tcagggtaaa acgatcgttt
    24661 agttgggtcg ttccctgata ataaaaactt tctttgtcat actctggcca ctgccagtag
    24721 cgtgatttct gaccactatt tccactgtat ggcgggttat ctttttcacc atcctgctta
    24781 atgtaagtca atgtgtattc atcgttttcg cgtggtgtaa aaccgagctt cacaatgccg
    24841 cgtttatcat cagccgatga attaatcatc ttgccgtgtt tgcctgcaat atcattattt
    24901 acaccatgcg gcaggccgag aaaatcctgc tttagctggc taccgctgac ttgcaaatac
    24961 cccagatcgc tgctggcggc aaatgaagca tgcatatcgt aggcattgtc ctggctacgg
    25021 ctccatccct gacgatatcc cagactggct tccagcggtt tagtcggctt ctgggtggtg
    25081 atattaatgg ctccgcccat ctgattaggc ccctgaagca gcgacgaata tcctttggaa
    25141 acttcaactg cccccaaatt gttggtcaga atccgcgcca gatcgaggtt gccgtcatag
    25201 ggaacataaa tgggcacacc gtcgaaatag atcggcacct gacgactatc aaagccacga
    25261 actttgacct gttcttcgtt gcggctacct gacttttgca gcaccacgcc ggggacgaca
    25321 cttaatgcct gagcgacatt ctgtttatcc agggccttca tggtgggttg atcgagaacg
    25381 gtggtcgtcg ttgatgatac cggactggac catacggtca gcgtgtcgct ctcctgagcc
    25441 gctacggcct gctgggtaaa ggcaagtgat aatactgtgc agagataacg ttttttgagt
    25501 ctcatagaat aaattccgtt taacaaagaa ttaaatagag gctgcgggtt gcagactgac
    25561 tttaatgtca gcaggtgccg cgtaataagg cgcagaggta ataaaaaggc gaatgccgca
    25621 gtcgaggtaa tttttgagtg ttgtcagatt aattccgccg gtcagagcca gcgtgcagtg
    25681 gggagccagc gacggtgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc
    25741 gagctgaagt acgtctggtt gcgcgcgtaa cgcggcgatt gcctctttcg gcgtgtcggc
    25801 ttcaacaact attttcttct cgggtgcgtg gcgacgtaac tgattgattg cgcctgacca
    25861 gtcctgattg tcatgaagaa aatgacggtg gttggcaaac aataatatgg tttccgcaca
    25921 tccggcacga tgaatcagtc ctccggcagc cagaattgcc tgcgaggcca gtaagcgggt
    25981 gcccggaatt gcttttcggg tgcaggcgat attgccatca gggtaacgtt cacgaagtaa
    26041 cgccagcatt tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac
    26101 tgccttccat ccctgatgaa gtgctgcggc attaccttgc gcacggatta gccgctgacc
    26161 cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa
    26221 catcttacac gcgacagaaa ttccgctgac gcagccgccc tgacggagaa aaaactctat
    26281 atagccatgc tggtgtccaa tatttaatgc tcgcgtggtc aggtcaccgc cctggatatc
    26341 ttccagaagt aacgcatcga tttgtgcctg agaaaggaaa atcatcgcag tgtctcctgc
    26401 ggtacgctat cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca
    26461 tcttgttgtt gataccagcg atataaccgc tcacgctcat cgtcgttaag cgcgcccaga
    26521 ctccagctga cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt
    26581 ccacggataa aatcaacgtg agcataaatt cccatctgat acaggacgtt gagtgcaaag
    26641 atatagttgg gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg
    26701 aacgaggtgc tgaccagatg agtcgtatag acgcgaagcc gtgcctgatt attcagtttg
    26761 ctcattgcct ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag
    26821 cgtggcaggt cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtctgcc
    26881 tttagcgcgg cggcgcgacg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca
    26941 taaatcgtcg tgagtttgtc cgccagcgcc agcgatacgg tacccggtcc gcagcccata
    27001 tcgaataagg tttgcgcgcc ttgcaggtca atttttgagg tcagttgttg caggtagctg
    27061 tccgtaggcg aggcgcaatt ttccgccatt ttttccgccc gttgatccca gtgatccggc
    27121 gttttttctg tgcgatgggc cagcctgagt tgctgaaggt aaaggtctgc aaaatcaata
    27181 tcgtcaatga gcatgatttt tccttagtgc ttaattgcag agaggtgatg gtgaatctgg
    27241 tcggcagaaa cccggtacag ggcagccagt tttttcgttg ttaactgttc ggtcggtaag
    27301 ccttgtgtta ctcgtccatc tggctctacc tggataatgc tgtcggcaat ggcgtttgca
    27361 tggagcggat gatgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc
    27421 gtgtccagaa gttggatctg atgaccgaaa tcgaggctgg aggcaggctc atccagtaat
    27481 aacaagcgcg gttgctggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg
    27541 ctaagcgtat tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc
    27601 gttgctttca ggcgttcttg cttaccggga acggaaaaag cgtctatggt gggggccagg
    27661 cccatcaaca ccatatccag cacactaaaa gcaaaaatgc cgtcgtgcgc ctgcggcacc
    27721 caggcaatag cctgcgcgcg ctggcgatgg ctgagtgtct gcacagggag gctatccagt
    27781 aagatctcgc cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg
    27841 ccgcaaccat tggctcctaa cagacaacaa attgttcctt gcgacagggt gaaggacacg
    27901 tcgcgtagca ccggatggcg ggagtaaccc agagacgcat gacggacggc aagcaaagtc
    27961 actggcgacc tccgcgtaac aacaaaaaca ggaaaaacgg tgcaccaaca aaagcggtca
    28021 gaatgccaag tgggatttct gttgttccaa tgctgcgtgc cagcgtatcg gtaagcagga
    28081 gcagaatcgc gccagtacac attgccatgg gcaacagctg ctgatggtta tgaccggtca
    28141 acaagcgggc aatgtgcgga acgaccaggc cggcccaacc aattatgccc gctattgcta
    28201 ccgtgctggc ggtgatgagt gtggcgcaga caatcaatcc aaagcgcaga cgtgtcacgt
    28261 tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga
    28321 gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta
    28381 aggtcacggt ggacagcccc cccagcaacc agaaggtaat tgacggtaac tgggtataag
    28441 gatcggccag cgtttttatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc
    28501 ctaccagcac cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga
    28561 cgcccgcgac caccatcagc ccgccgcaaa atgcgtacag ctggatgtaa acaatggata
    28621 atccccacag gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat
    28681 ccggtgacac taagggatta cggaacattc cctgataggt ggttccggcc cctgccagcg
    28741 ccgcgcccag aaacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt
    28801 gttcttgcac aggaacattt tcttgacgaa gaatgagcgc cagcagttgc tgcatatcga
    28861 gatgataagc cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac
    28921 atccgtttac ggttctcatt agctcgccac caatttttga tagtcggcgt cactgagtga
    28981 acaatgccag aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc
    29041 gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc
    29101 atccagccag ccaaagggca ggccacttaa aaataagatg cgttgttccg caaccgcttt
    29161 cacgccctgc cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag
    29221 aataatatcc ggctgccagc gcaggagatt ttccatggaa acctgtgtca gaccgtggcg
    29281 atcggctatt tgcgcgacgt tatgcaaacc taataattcc gccgtctcgg tatgcaacga
    29341 accctgtaag cccgtttcca gccctcgagg accgcgcgca gcataaaagc tgaggttagc
    29401 ggcgggtgag gtggcgaatg cctgagcttc accaacgaag cggctggcga gattggcttg
    29461 ttctgcggcg cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga
    29521 ttgttccagt ttcccgttaa gcaataacca gggtatttgt gtctgttcgc taacctgccg
    29581 tgcctgggag atccaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa
    29641 cgccataagc ccttccagcg agagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg
    29701 gaactggcga atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga
    29761 aaaaccgacc attttttctg gcgcgacagc cagcaatagt aaatcggcgg gcgcgcctgc
    29821 gctgacgatg cgtgtaatgt tttgggcttc gggaagcgtc agtgaggccc agaatttgac
    29881 tgattgtgca aaggacggca gatgatgcag gaacaatgtc gacgcaagaa tctgtgtaaa
    29941 cctccgtctg gttagtgaca taagtaatat cctgataatt aaaaccgcta tatacttaca
    30001 catatagcga aaaagatcat gtttgatgtc aaaatttgtc taaagagaca gaatgatttg
    30061 cttgtttctt aaataacact ttgttccaca agaaaattgt cagggagaat ggggagtgg
//
LOCUS       DADTFG010000046        30108 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.046, whole genome shotgun sequence.
ACCESSION   DADTFG010000046 DADTFG010000000
VERSION     DADTFG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.046"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(8..3889)
                     /gene="entF"
                     /locus_tag="J1138_001919"
     CDS             complement(8..3889)
                     /gene="entF"
                     /locus_tag="J1138_001919"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBA8453722.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSTWSVAHYVELTGEVDAPLL
                     VRAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPSLLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAVKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLKDARPSLLITTDDQLPRF
                     SDIPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTSANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMTPEQAWAPWIAGL
                     DIYRQDCAHVDIISPVAFEKIGPIIRATLNK"
     gene            complement(3886..4104)
                     /locus_tag="J1138_001920"
     CDS             complement(3886..4104)
                     /locus_tag="J1138_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HBA8453723.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEVQ"
     gene            complement(4107..5309)
                     /gene="fes"
                     /locus_tag="J1138_001921"
     CDS             complement(4107..5309)
                     /gene="fes"
                     /locus_tag="J1138_001921"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBA8453724.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPRSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLALREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNTDFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5552..7792
                     /gene="fepA"
                     /locus_tag="J1138_001922"
     CDS             5552..7792
                     /gene="fepA"
                     /locus_tag="J1138_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBA8453725.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            7967..8587
                     /gene="entD"
                     /locus_tag="J1138_001923"
     CDS             7967..8587
                     /gene="entD"
                     /locus_tag="J1138_001923"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBA8453726.1"
                     /translation="MKTTHTALPFAGHTLHFVEFVPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTARELTDNIITPAEHKRLADCGLAFPLALTLAFSAKESAFKASEI
                     QTDADFLDYQIISRNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8709..8861)
                     /locus_tag="J1138_001924"
     CDS             complement(8709..8861)
                     /locus_tag="J1138_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA8453727.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9313..10431
                     /locus_tag="J1138_001925"
     CDS             9313..10431
                     /locus_tag="J1138_001925"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBA8453728.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLQNFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKITPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            10497..10745
                     /locus_tag="J1138_001926"
     CDS             10497..10745
                     /locus_tag="J1138_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBA8453729.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10810..11193
                     /locus_tag="J1138_001927"
     CDS             10810..11193
                     /locus_tag="J1138_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBA8453730.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            11272..11925
                     /gene="nfsB"
                     /locus_tag="J1138_001928"
     CDS             11272..11925
                     /gene="nfsB"
                     /locus_tag="J1138_001928"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBA8453731.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12033..13280
                     /locus_tag="J1138_001929"
     CDS             12033..13280
                     /locus_tag="J1138_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBA8453732.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13437..14819)
                     /gene="pheP"
                     /locus_tag="J1138_001930"
     CDS             complement(13437..14819)
                     /gene="pheP"
                     /locus_tag="J1138_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBA8453733.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(14921..18064)
                     /gene="cusA"
                     /locus_tag="J1138_001931"
     CDS             complement(14921..18064)
                     /gene="cusA"
                     /locus_tag="J1138_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBA8453734.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18076..19299)
                     /gene="cusB"
                     /locus_tag="J1138_001932"
     CDS             complement(18076..19299)
                     /gene="cusB"
                     /locus_tag="J1138_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBA8453735.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAELPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRVGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19315..19647)
                     /gene="cusF"
                     /locus_tag="J1138_001933"
     CDS             complement(19315..19647)
                     /gene="cusF"
                     /locus_tag="J1138_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBA8453736.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGSLSL
                     LQDIKVSQ"
     gene            complement(19671..21053)
                     /gene="cusC"
                     /locus_tag="J1138_001934"
     CDS             complement(19671..21053)
                     /gene="cusC"
                     /locus_tag="J1138_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBA8453737.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLPSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21210..21893
                     /gene="cusR"
                     /locus_tag="J1138_001935"
     CDS             21210..21893
                     /gene="cusR"
                     /locus_tag="J1138_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBA8453738.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21883..23325
                     /gene="cusS"
                     /locus_tag="J1138_001936"
     CDS             21883..23325
                     /gene="cusS"
                     /locus_tag="J1138_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBA8453739.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLLSLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLGDEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDVRGTRFVIILPA"
     gene            23475..25712
                     /gene="nrfB"
                     /locus_tag="J1138_001937"
     CDS             23475..25712
                     /gene="nrfB"
                     /locus_tag="J1138_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBA8453740.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            25699..28671
                     /gene="nfrA"
                     /locus_tag="J1138_001938"
     CDS             25699..28671
                     /gene="nfrA"
                     /locus_tag="J1138_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBA8453741.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSMAAIPVEVKRVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADALYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYGA
                     AQKLLATLPANEMLEERYTVSVATHNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSSAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYVRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            28672..29562
                     /locus_tag="J1138_001939"
     CDS             28672..29562
                     /locus_tag="J1138_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBA8453742.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFIAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPAAQSILEY"
BASE COUNT         7279 a         8155 c         7688 g         6986 t
ORIGIN      
        1 tataaattta cttatttagc gtcgcacgaa taatcggccc aattttttca aacgccaccg
       61 gagagataat atccacatgc gcacaatcct gacgatagat atccagccca gcaatccacg
      121 gtgcccaggc ctgttcagga gtcatacctt cctgaagcgt gcgttcggca acaaatagcg
      181 tcgctttacc atcaaacggt acgctatgag ctgtcgtcag caggcgcaca gcatcagcgt
      241 agttgccttc aatggtggta aacaactccg ttgaagtact tccctgctgt gccgccagga
      301 aggcttcgcg ctcgcggtta atctccgcca gcacttccgg gtccagacca ttagcttctt
      361 tttcctgcca gttttgcgtt tctggcggcc aggtatccag caagccaaga aatgccacct
      421 gttcgccacg ggcacgcagt cgcgccgcaa tgccctgcgc cagcgtaccg cccagcgaat
      481 aacccagcag gtaatatggg ccgtgcggtt gttgttcaag taacgttgcc agatgcgctt
      541 cgcagacttc atccagattt gctgacgtct gcatggggcc atgagggcgc ggcgactgaa
      601 tgccgataat cgaccattgt ggatcgagat aacgcgagag cacgctgaac tgccaggcaa
      661 aaccggatgc cggatggaaa caaaacagcg tcggaccatt accttcacgc aacggcagaa
      721 tggtttcgaa tcccatgcgc tgggtgctgt cttcttcacc atcaataatc gttgccagtt
      781 tggcgacagt tgacgcgacc atcacctgcc ccggcgtcac ctgacgagca aactgtcgac
      841 ttaactgcgc tgccagtttc attgccagta gcgaatgacc gccaagcgca aagaaatcag
      901 catcggcatc ctgcacgtca caacccagca acgacgagaa tgccgcggcg ataatcgttt
      961 cactgcccgc tttcggcgca cgcccaggcg cttgtgcctt cagttcaggc aacggtaagg
     1021 ctttgcgatc cagcttgccg ttggcgctaa gtggtaactg tggaagttgc agcagaacca
     1081 ccggtaccat atgtggtggc aatgtttcac gaagctgcgc ctgtaatgcg ctggtatcca
     1141 acggcagacc tgattgcgac accagatagc ccaccaactg acgcgcatca ccaccagtgg
     1201 ctgccgcctg gttaatcaca caggcgtggg taacggcttg ttcgacatcc ggcagcgcct
     1261 gcatcacgcg atcaatttcg cccagttcga tacgctgccc gcgaattttt agctgatcgt
     1321 cactgcgccc gaggtactcc actgcgccgt tatccagcca gcgagccaca tcgccggtac
     1381 ggtacatccg ttcacctggg gcaaaaggat cggcaataaa gcggctggcg gtcagatcgg
     1441 gtcgtccaag atacccctgc gccagttgaa taccggtgag atagagatct cccgccacac
     1501 ccggcggcac cggatgcatc atcgcatcga gaatgcgcag gcccgtattc cacaccggat
     1561 aaccaatcgg cacactgctg ccgcgcacct ctgccagttc ctcgccaaaa gccggatacc
     1621 aactgacatc taccgccgct tccgtcgggc catatagatt atgcaacggc gcgccggtta
     1681 actgttgcca ttcgcggcat aaatcagccg gtaaggcttc accactacag aaaacctgtt
     1741 tcaacgtcgc gcaactctgg cgagcggttt gcggcgtcag cgatgcaaca aatgccgcca
     1801 gcatcgacgg cacaaagtgc gtggtcgtta cgccatattc ggcaaagaat tgctgcatag
     1861 cgagcgggtc gcggtgcgct tccggttcag ccatcaccag ttttgccccg gcaataaatg
     1921 gccagaaaaa ctcccacacc gagacatcaa aactgcacgg cgttttttgg gcaacgacat
     1981 cttcgcctgt aagcgggtaa tggttttgca tccaaagcag gcgattaacg atagccgtct
     2041 gcccgaccat tacccctttt ggtctgccgg tggaaccgga ggtgaagatg atataagccg
     2101 tgtggtgcgg ttgtgaaagc tgcagcggcg cactgccctg cggtgtaagc ggggcgttat
     2161 agcaaaggct ggttaaatta gggatatcgc taaagcgcgg cagttgatcg tcagtggtga
     2221 ttaacagcga cggacgcgca tctttcagca tcattttcag gcgatcgtcc ggatagccgg
     2281 tatccaacgg taaccaggcc gcaccagctt caactatcgc atgtagcgcc agggtcaaaa
     2341 agaccgagcg cggtagagcc accgccacgc tgtcgcccgg tttaacgccg cgctcacgca
     2401 gcagattcgc cagcgccacc acctgctcgc gcatttcccg atagctgaac tggtaacgcg
     2461 catctgccag cgccggagca tccggtgttt ttaccgcttg ttctgccacc agcgcgctca
     2521 gcgtggtttc tggaatctca acctgagtgg cgttgatctg cgccagctgc gcatactcgc
     2581 ctggcagcat aatatcgaca tcgccgcaca acagcgacgg atccgcagcg aactgcgcaa
     2641 tcagcatttt caggcgttca gcgtgttgaa ttaacgttgg ctcatcgtaa cgctgtttat
     2701 tggcgaggat ttcaatactc aaatcaccgt gttcatccgg gaacagggcc agttcaaggt
     2761 cattaaccgg accggttgcc agggtatggg tttgcgcctg aacaccagga atatccagtt
     2821 ggtaatcaaa taccttgata ttgagtaccg gaccgaacag cggttcctct cctgccgctc
     2881 gcccactgtc acggacaatt tgttcggcgt cgtagcgttg atggcggcgc atttttttca
     2941 gttgagccgc cagtcgggtt gccagttgcg gcagcgtttc ttgcgccgca atgtgaatac
     3001 ccaacggcaa aacgttgagc acgggtccgg tagccgtcag cgccgccgag cccagtcgac
     3061 gcataaagat aaatccggca gcgtagtcca tgcggttgca caatcgcccc agccacaaag
     3121 ctgccaacgc aagggctaaa tcggtacgct gcacacctga aagttgcgta gccagctggc
     3181 ggaattcccc gtcggtaaat tccagtttca ggcgcaaaat atctgccgaa gcgctgcgcc
     3241 ccggtaaagg tgccggagaa agtgacgcgg gcggtggcag ttgacgacgc tgttccgccc
     3301 agaatgccgc atcacgctgc caggcttcac tttcgcggta ctgctgatac tcttccacta
     3361 catcagcgaa aggcgtaaac ggcgaagcag gcgttggttc gccacgcagc catgcgcagt
     3421 aaatattggc gatctggcgg gtaatggccg ggaaactgaa gccatcgacc agcaaatggt
     3481 gataacgctg ataccagtac cagcggttat ccgccacctg tatcaactga tgaaagacca
     3541 gtggtttacc gctatcgacg cgcagatctt gttgcaaatc cgcctgcatt aatgcccgcg
     3601 cagtaccgtg cggatcaatg ttggttcgca ggtcgataat ttctggcagt tcgaacgtca
     3661 gtgcatcatc gacccactgc cagacttcgc cgttatcttc cgtaaaacgc atccgcagcg
     3721 tatctgcttg cgctaatccg gcaaccaccg cgcggaccag taatggcgca tcaacctctc
     3781 cggttaactc aacgtaatgc gccacgctcc aggtggaggg taaatccgac agtttttctg
     3841 ccatccagat gccgggctgt gcggcgacca aaggtaaatg ctggctcatt gcacctcctg
     3901 caactgggtg aaattcgtcg gtgtcagagt acgccagtgg gcttccagcc actgctggca
     3961 ggacgcctgt gactgcggct gacacacaac gtcccagcct gccggtaagg cgcattgttg
     4021 cggccacaga ctgaattgcc cctgcgcatt gcgcaaaatg taaaacgctc cctgcggatc
     4081 atcgaaggga ttactgaatg ccatattcaa ctcctgtcat ggaaaagtgg ttgccagagg
     4141 tcgattagcc cctgcatcaa gccaccgcgc caacaaagcg catcatgtcc gccgtcaacc
     4201 tgacgccaga aaatggattc ttttataggg tgtaattgcg catacagcgc ctgattggcc
     4261 cgcatgatca tcggctcgcg aatacccgct tccagcacaa tgcgcagacc ttcggcgcta
     4321 acttcaccag ctttaagctt ttcaagtaac acgccctctt gctgcccgcc ccgatgcggc
     4381 caccagtacg atcctgactg gcttaataca cagccaaagc gttcaggcca gtgcagtccg
     4441 gcatacagcg cggaaagtcc accaaaactc tgcccggcga ccacggtgcg atcggcgcga
     4501 tcgctaaaag gggcaatagc tttcaccagg ggtaataact cttgctgtac tgccagccag
     4561 aaatccgtat tacacggcag ttcgtgggca cggtgcgtgg tgtcgatagc gtcgatcaac
     4621 acatacacgg cgggaggaag ttgctgacga tgggtcagcg aagtcagcac aggccagacg
     4681 ggcatacttt gcgcccaaaa ttcgccatcg agcaaaactg ccagcgggcg ttcttctgct
     4741 gttgcatcgc cggtggtaaa aatccataca cggcgtgaat ttttcaaccg ttcacttttc
     4801 cagataattt ctttggcagg tgtttctggc gcttgcggac aatcccaacc cggttgcagg
     4861 ggcgcttgcg gcatttcgag tgcagaaaca gcgtgccctc gcccgccttt ccagctttgt
     4921 ggattaagcg gatcggctat cgcctggggt aatagttttc gccagccttc gcgcaatgcg
     4981 aggcgatcgg gggatggtgc agaaaaaatg tcatcgcgtt cggtgggaat aaagcagtag
     5041 ctgccgcgcc agttggcatt gagttgtgtc gtccactgcc agacgttggt gcctgcaatt
     5101 cgctgcatcg accggggctg gctgttctga tggtgatcgg tcacaccagt gatgtagacc
     5161 catacgcgct ttatcgtcga gtattcttca gaaccctggg gatcacgcca ccagaaagtg
     5221 acctcaaaca tttcgtcatt cagacgctgc cattccgggc catgtttcga ctgccaccag
     5281 ctctcacttc ctacttttaa cgccgtcaca ccataacccc atgtttactg tgcaattttt
     5341 tattgattgc agaaatatat tgataatatt attgataact atttgcattt gcaatagcgt
     5401 aatggcgcgc cgtgggaagc gcggacatta attaaccaac tgcactgcgt gtctttcagg
     5461 atcaaaggtt ttcgcggtag cgggatgcgt cgtgttgatg acgaccatgc ccgacagttg
     5521 caattcgtgg caaaaatgca ggaataaaac aatgaacaag aagattcatt ccctggcctt
     5581 gttggtcaat ctggggattt atggggtagc acaggcacaa gagccgaccg atactcctgt
     5641 ttcacatgac gataccattg tcgttaccgc cgccgagcag aacttgcagg cacctggcgt
     5701 ttcgaccatt accgcagatg aaatccgcaa aaacccggtt gcccgcgatg tatcggagat
     5761 cattcgtacc atgcctggcg ttaacctgac cggtaactcc accagtggtc agcgtggtaa
     5821 taaccgccag attgatattc gcggcatggg tccggaaaac acgctgattt tgattgacgg
     5881 caagccggta agcagccgta actctgtgcg tcagggctgg cgtggcgagc gcgatacccg
     5941 tggtgatacc tcctgggtgc cgcctgaaat gattgaacgt attgaagttc tacgtggtcc
     6001 ggcagctgcg cgttatggca acggcgcggc gggcggcgtg gttaacatca ttaccaaaaa
     6061 aggcagtggt gagtggcacg gctcctggga tgcttatttc aatgcgccag aacataaaga
     6121 ggaaggtgcc accaaacgca ccaacttcag cctgaccggt ccgctgggcg acgaattcag
     6181 cttccgcttg tatggcaacc tcgacaaaac ccaggctgac gcgtgggata tcaaccaggg
     6241 ccatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta ccagccgggc gcgaaggggt
     6301 gatcaacaaa gatattaatg gcgtggtgcg ctgggacttc gcgcctctgc agtcactcga
     6361 actggaagcg ggctacagcc gccagggtaa cctgtatgcg ggtgatacgc agaacaccaa
     6421 ctctgacgct tacactcgct cgaaatatgg cgatgaaacc aaccgcctgt atcgccagaa
     6481 ctactcgctg acctggaacg gtggctggga taacggcgtg accaccagca actgggtgca
     6541 gtacgaacac acccgtaact cgcgtattcc ggaaggtctg gcgggcggta ccgaagggaa
     6601 atttaacgaa aaagcgacac aggatttcgt cgatatcgat ctcgatgacg tgatgctgca
     6661 cagcgaagtt aacctgccga ttgatttcct cgttaaccag acgctgacgc tgggtacgga
     6721 gtggaatcag cagcggatga aggatttaag ttccaacacc caagcgctca ctgggacgaa
     6781 taccggcggt gctattgatg gtgtgagtgc caccgaccgt agcccgtatt caaaagcaga
     6841 aattttctcg ctatttgccg aaaacaatat ggagctgact gacagcacca tcgtaacgcc
     6901 ggggctgcgt ttcgatcatc acagcattgt tggcaataac tggagtccgg ctctaaacat
     6961 atcgcaaggt ttaggcgatg acttcacgct gaaaatgggc attgcccgtg cttataaagc
     7021 gccgagtctg taccagacta acccaaacta cattctctac agtaaaggtc agggctgcta
     7081 tgccagcgcg ggcggctgct atctgcaagg taatgatgac ctgaaagcgg aaaccagcat
     7141 caacaaagag attggtctgg agttcaaacg cgacggctgg ctggcgggcg tcacctggtt
     7201 ccgtaacgat tatcgcaata agattgaagc gggctatgtg gctgtagggc aaaacgcagt
     7261 cggcaccgat ctctatcagt gggataacgt accgaaagcg gtggttgaag gtctggaagg
     7321 atcgttaaac gtaccggtta gcgaaacggt gatgtggact aataacatca cttatatgct
     7381 gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc atcccggagt atacgttgaa
     7441 ctcaacgctg agctggcagg cacgggaaga tttgtcgatg caaacaacct tcacctggta
     7501 cggcaagcag cagccgaaga agtacaacta taaaggtcag ccagcggttg gaccggaaac
     7561 caaagaaatc agcccgtaca gcattgttgg cctgagcgcg acctgggatg tgacgaagaa
     7621 tgtcagtctg accggcggcg tggacaacct gttcgacaaa cgtttgtggc gtgcgggtaa
     7681 tgcccagacc acgggcgatc tggcaggagc caactatatc gccggtgccg gggcgtatac
     7741 ctataacgag ccgggacgta cgtggtatat gagcattaat actcacttct gatgctaacg
     7801 tcagattgtt gacaaagtgc gcgtagttca tgccggatgc ggcgtgaacg ccttatccgg
     7861 cctacaaaaa tcttgccaat tcaatatatt gcaggaccac gtaggcctga taagcgtagc
     7921 gcatcaggca gttttgcgtt tgtcatcagt ctctaatatg gtcgacatga aaactacgca
     7981 taccgccctc ccctttgccg gacatacgct gcattttgtt gagtttgttc cggcgagttt
     8041 tcgcgagcag gatttactct ggctgccgca ctacgcgcaa ctgcaacacg ctggacgtaa
     8101 acgtaaaaca gagcatttag ccgggcggat cgctgcagtt tatgcgctgc gggaatatgg
     8161 ctataaatgt gtgcccgcaa tcggcgagct acgccaacct gtgtggcctg cggaggtata
     8221 cggcagtatt agtcactgtg ggactacggc attagccgtg gtttcccgtc aaccgattgg
     8281 cattgatatt gaagagattt tctctgcaca aaccgcaaga gaattgacag acaacattat
     8341 tacaccagcg gaacacaagc gacttgcaga ctgcggttta gcctttcctc tggcgctgac
     8401 actggcattt tccgccaaag agagcgcatt taaggcaagt gaaatccaaa ctgatgcaga
     8461 ttttctggac tatcagataa ttagccggaa taaacaacag gtcataattc atcgtgagaa
     8521 tgagatgttt gctgtgcact ggcagataaa agaaaaaata gtcataacgc tgtgccaaca
     8581 cgattaattg acaacatctg gtacgattcg cccgcagcca tctctgactg cgggcgaaag
     8641 tgtaaagcag gtgccttacc atcctgacct gacaaccgga tatgcgggga ttactccccg
     8701 cacaacggct acttcttcgg ttcgtaagcg agaacagcct taaactcaat attacgttcc
     8761 tttacggtaa actcacacag cgagtctccg accagaagcg taaatcccag caccgttaaa
     8821 cacagcacta tgactgccac aagggcatat ttcgtcagca tgtttatatt gcctccatgc
     8881 gaagaaggga ctaccatcca aattgttcag gtttgggtag gtagccccag ggttgataga
     8941 aatatcgtcc tggggcttca tccttccaga cctcagagta aacctgaaac cggaaagctt
     9001 caggcacccg ccgttatctt acccttcttc agcaaaatag ctgtttattt atacagtaag
     9061 tcctgtttcg gacttctctc tcctcatttg atcaactgaa gctgaacccg tatttttctg
     9121 catgccgcaa tcgtcaaaat tcttcgcagc cagccagctc tgccataact tggcacattg
     9181 caaaagtagc caaattgctt cagtagcaat cagttcacca tacccaaaaa agaaacattc
     9241 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgccaaca
     9301 taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac cctcggtatt
     9361 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
     9421 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
     9481 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcaattt
     9541 tcagcgatgc agaaagtcgt attgcaggca gccgcagatc atcatctgga aatttgcggc
     9601 ggtggcacgc atccgtttca gaaatggcag cgtcaggagg tgtgcgataa cgaacgctat
     9661 caacgaacgc tgcaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
     9721 gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
     9781 tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaagg aacggatacg
     9841 cgttttgcct cctcacgacc gaatattttt tccgcctttc ctgataatgg cccgatgccg
     9901 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gcctgagtta caccacgatg
     9961 atcgacagca ttaaagatct gcactgggat attcgcccca gcccccattt tggtacggtg
    10021 gaagttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta
    10081 attcaggcca ccgcccactg gttactgacg gaacgcccgt ttaaacatca ggagaaagat
    10141 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga aggcgtcatt
    10201 accgatccgc atactggcga tcgtcggtca ctaacggaag ccaccttgcg tttactggaa
    10261 aaaatcaccc cttctgcaca taaaattggc gcatcgagcg caattgaagc actgcaccgt
    10321 caggtcgtca gtggtctgaa tgaagcgcag ctaatgcgtg atttcgtcgc cgatggtggc
    10381 tcgctaattg ggctggtgaa aaagcattgt gagatctggg ccggagaata agtcgggaat
    10441 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
    10501 aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct ttcctctctt
    10561 gcctgctgct gcccgccccc gcactggggc taacgctggc acaaaaactg gtgaccacgt
    10621 tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg tttttagtgc
    10681 tgggcgctat tgagtatttt gttctacgct ttatctggcg acgctggttc tcgctggcgg
    10741 attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc ttcgcctaaa
    10801 ggaacattta tggataagca atcactgcac gaaacggcga aacgcctggc tcttgagtta
    10861 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
    10921 aagattttta tgctgtcgtc ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat
    10981 ccacaaaaat ccctgctaaa tcagcaaata tacccaagca tcaagcctgg gtatcacatg
    11041 aataaaaagc actggatttc agtgtatccc ggcgaggaaa tctccgaagc gttacttcgc
    11101 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
    11161 cgactgcgtc ctgactcaac cgtaaatcaa tagcgcattt ttctcgctta ccatttcatc
    11221 tcgatccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt tatggatatc
    11281 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa aaaacttacc
    11341 ccggaacagg ccgagcagat caaaactcta ctgcaataca gcccatccag caccaactcc
    11401 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
    11461 gctgccgata attacgtgtt caacgagcgt aaaatgcttg atgcctcgca cgtcgtggtg
    11521 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
    11581 gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
    11641 ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
    11701 tatctcaacg tcggtaactt cctgctgggc gtggcggcgc tgggtctgga cgcggtgccc
    11761 atcgaaggtt ttgacgccgc catcctcgat gaagaatttg gtctgaaaga gaaaggctac
    11821 accagtctgg tggttgttcc ggtgggtcat cacagcgttg aagattttaa cgctacgctg
    11881 ccgaaatctc gtctgccgca gaacattacc ttaaccgaag tgtaattctc ccttgccggg
    11941 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
    12001 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
    12061 atttagcggg tattgagatt gatcacacca cctcgatggt gatgattttc ggtattattt
    12121 ttctgaccgc cgtcgtggtg catatttttt tgcactgggt ggtactgcgg accttcgaaa
    12181 aacgcgccat cgccagttca cggctttggt tgcaaatcat tacccagaat aaactcttcc
    12241 accgtttagc ttttaccttg caggggatta tcgtcaatat tcaggcggta ttctggctac
    12301 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
    12361 atgcgctgct ttcagtcttc tcgttgctgg atgtgatttt gaatctggcg cagaaattcc
    12421 cggcggcatc tcagttaccg ctgaaaggga tttttcaggg gattaaactg atcggcgcga
    12481 ttctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
    12541 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
    12601 tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaga
    12661 tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtac
    12721 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
    12781 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
    12841 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
    12901 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
    12961 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
    13021 atctgaacga atatctgcgg aatcatccgc ggattcgcaa agatatgacc ttaatggtac
    13081 gccaactggc tcctggtgat aacggtttac cgcttgagat ctatgcgttt accaacaccg
    13141 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
    13201 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
    13261 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggtttc
    13321 ggatggttga catcattttg tcggatgcgg cgtgaacgcc ttatccgacc tacagaatcg
    13381 ttacaaattg taggcccgat aagcttgcgc atcgggcaaa tcagcctcac atcagcttaa
    13441 ttcactttcc tacgcaatgt tttaaacgcc acaaacagga acacaatcca caccggcagc
    13501 agaatcgctg acaagcgcat atcatccatc gtgcacatca gcagcaaaat catgccgagg
    13561 aaggcgatgc aaagatagtt gccgaacgga tagagcagcg ccttaaactg tgtttcacgc
    13621 ccctgacgac gcatcgctgc acgaaaacgc agatgcgcca ggcagatcat aatccagttc
    13681 aacagcagcg ttgctaccac cagcgccatc agcagaccaa acgctttttg cggcagcaga
    13741 tagttgatta acaccaccag cgaagtgatc gctccggaaa gcatcagcga gttaatcggc
    13801 acgccgcgac ggctgacgcg agtcaaaaac tttggcgcat taccctgcac agaaaggcca
    13861 aacagcatgc ggctgttaga gtaaaccccg ctgttataca ccgacagcga tgccaccaga
    13921 atgacgaagt tcagcgcaga agctaccacg ttgctgtcga gattatggaa aatcatcaca
    13981 aacgggctac tgttggattt cacttccacc cacgggtaga gcgccagtaa aaccaccagt
    14041 gaaccgatat aaaacagcag gatgcgatac accacctgat tcactgcttt agggatgctt
    14101 ttttccggat cgcgcgcttc agccgcagta atcccaatca gctccagacc gccgaaggag
    14161 aacataatta ccgccagcga caaaatcaat ccattccagc cggtggcaaa gaaaccgccg
    14221 tagcgccaga ggttatcgat actggctttc tcaccgccgt gcccagaaaa cagcagccac
    14281 aggccaaagc cgatcatacc gatgatcgcc agcactttaa tcagcgcaaa ccagaactcg
    14341 gtttcgccat ataagcgcac gttcaccagg ttaacggcgt tgatgataat aaagaaggcg
    14401 gcagcccaaa tccacgttgg aacatccggg aaccagtact gcatatagat gcccgcagcg
    14461 gtcagctctg ccattcccac cagcacgaac attacccagt agttccagcc agagaggaag
    14521 cccgcaaacg gtccccagta tttataggca aagtgggcaa atgaaccgga taccggctct
    14581 tcgaccacca tctcgccgag ctggcgcata atcaggaaag cgatgatccc ggcgacgccg
    14641 tagcccagca atacagccgg acccgccatc tgaatcgccg ggccaatgcc aagaaacaga
    14701 ccagtaccaa ttgcgccacc cagcgcaatc agttgaatat gacggttatg taatccgcga
    14761 tgaagcgtcg gctcttgatt cgacgcagta tcttccgata cggttgacgc gtttttcacg
    14821 cctttcccct gtgtgtcttt tttgttgagg ggcacctttt aacatttagt gcccatcgtc
    14881 gcaagacaca atccacacgg ttaaacgggg tatcctgctt ttatttccgt acccgatgtc
    14941 ggtgcagcca catcagctta tacgccgccg ggataataaa cagcgacagc aaaggcgcgg
    15001 tgatcatgcc gccaatcatc ggcgcggcaa tccggctcat cacctctgac cctgctcctg
    15061 ttccccacag aatcggcagc agaccagcga taatcaccgc caccgtcatc gcttttgggc
    15121 gcacgcgcag taccgcgccg tgatataacg cctcatccag cttctgctcg ctgaatgttt
    15181 gcggattatt caacgacggc tcggcctcta tggcgtgacg taaatacatc agcatcacca
    15241 cgccaaattc ggcggcgacc ccggcaaggg caataaaacc ggtgcccgtc gccacggaaa
    15301 gatgaaagcc catccaccac agcaaccaga tgccgcccac cagcgcaaac ggtacgctgc
    15361 tgataatcag caacgcttcg cccacccgac ggaacgccag atacaacagc acgaagatga
    15421 tcatcagcgt catcggcacc atcagtttca gcttatggtt ggcgcgctcc agtagctcga
    15481 actgcccgga gaatgccacg ctagtgcccg gttttaactg cactttttca gctatcgctt
    15541 tttgcagatc gtgaaccacc gacaccatgt cacgatcgcg ggcatcgata taaatccagc
    15601 tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc ggtagagact ttaacgtcgg
    15661 ccacgtccgc cagagtgatt tgctgcttca tcggcgtgag gatcggcaac tggcgcagtg
    15721 cctgcgggct atcgcgccag ctttgcggat aacgcagatt aattggataa cgagcaatcc
    15781 cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga tgtcacaaac aattgcacat
    15841 ccgccaccgt cataccgtaa cgcgcggctt tttcacggtt aatctcaacg ttgatatagc
    15901 gcccaccttc cagacgctca gcaagcgcag aagccacgcc aggcaccgtt cgtgccactt
    15961 cttcaatttg ctccgccatc gcgtcgatat ccgccagcac agtgccggaa actttaatgc
    16021 cgatagggct tttaatgccg gttgagagca tatcgatacg gttacgaatt ggcggcaccc
    16081 acagattcgc cagccccggc agacgcacgg tgttatccag ttcctcaatg attttgtcca
    16141 tcgtcatgcc tggccgccac tgatcctgcg gcttaagctg gatggtcgtt tctaccatct
    16201 ccagcggagc agaatcggtg gcggtttccg ctttcccggt tttgccaaat acccgcgcca
    16261 cttccggtac gctcataatc agcttgtcgg ttttttgcag catactcgcc gcctccgctg
    16321 cggaaatccc cggcagcgtc gatggcatat acaacaagtc gccttcattg atctgcggta
    16381 aaaattcccc gccaacttta ttgagcggcc agagaaccgt cagcaccgaa agcgctgcca
    16441 ccaacagcgt ggttttcggc cagtgcagta ctttcagcaa cagcggatga taaacacgaa
    16501 tcaaaaagcg attgagcggg ttactgcttt ccggcggaat tttgccacgg atccagtagc
    16561 ccatcaggat cgggatcact acaatcgcca gtagcgccgc gcccgccatc gcataggttt
    16621 tggtgaacgc cagcgggcca aacagacgac cttcctgccc ttccagggtg aagatcggga
    16681 taaacgacag cgtgataatc agcagactga taaacagcgc cggccccact tcaacagatg
    16741 catcggtgat cacctgccag cgcgttttat tatccagcgt ggcgtcagga tgctggtgct
    16801 gccactcttc cagccgttta tgcgcattct cgatcatgac gatagcggca tcgaccatcg
    16861 ccccgacggc aatcgcaatg ccacccaacg acataatatt ggcgttcagt ccctgaaagt
    16921 gcatgacaat aaaagcaata cacaacccca gcggcaacga aataatcgcc accagcgccg
    16981 agcgcacatg ccagagaaac agcgcacaga ctaccgccac cacaataaac tcttccagca
    17041 acttgccgct gaggttgtca atggcgcggt caatgagctg gctgcgatcg tatgttgtaa
    17101 ctatctccac gccttccggc agactgcttt tcagcgtttc cagtttgtcc ttcacggcgg
    17161 cgatcacttc acgggcgttt ttgccggaac gcaggatcac caccccgccc gccacttcgc
    17221 cttcgccgtt cagttcggca atgccccggc gcatctccgg gccaacctgg accttcgcaa
    17281 catcacgcag ataaacgggc acgccatttt cactggcttt taaaacgatg tgattaaagt
    17341 cgtcgagcgt ttgcagatag ccgctggcgc gcaccatata ttccgcttcc gccagttcga
    17401 tcgacgaacc gcccgcttcc tggtttgaag catccagcgc gctttttact tcggcgagac
    17461 tgatgccata ctgcgccagg cgctgaggat cgataaccac ctgatactct ttcaccacac
    17521 cgcccaccga cgccacttcc gcaacgtcag ggatggtttt tagctcatat ttgagaaacc
    17581 agtcctgcaa tgagcgtaaa tcggccagat cgtgcttacc gctgcgatcc accagtgcat
    17641 attcatagat ccagccaaca cccgtggcat ctggccccag ctcggcgctg actcccgcag
    17701 gcagcttacc ctgtacctgg ttgaggtact ccagcacccg cgagcgcgcc cagtacggat
    17761 cggtgccatc ttcgaaaatg acatacacat aagagtcgcc aaactgcgag aaaccgcgca
    17821 cagtcttcgc gccaggcacc gacaacatgg tggtggttag cggataagtc acctgatttt
    17881 caacgatttg cggtgcctga ccgggatagc tggttttaat aatcacctgt acatcagaga
    17941 gatccggcag tgcatccact ggcgtgttaa tgatggtcca ggtgccccag atactcagaa
    18001 acaacgcgcc catcagcacc agaaaacggt tcgccaccga gcgacgaata atccattcaa
    18061 tcattggcta ttccctcaat gcgcatgggt agcactttca gagcgcatcc gctccagtgc
    18121 gccagaaata ttggcttcag aatcaatcag gaacaggccg ctggaaacca ccttttcacc
    18181 ttccgccaga ccagagcgta acgcggtgac gccttgcgat gcctggaaaa cggcaacgcg
    18241 tttcggtaca aagcgcccgt cagcatcaac ggtaatcacc cgctgttcgt tgccggtatc
    18301 aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg gcggtattga gttgcaacca
    18361 ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg acttccagac gcagttgcag
    18421 cgtgcgggtc gcagcatcca cgccaggtag cagcgtccat ttgcggatgg tgagtgtttt
    18481 atcaggtcgc gccggaacgg tgagcgtaaa ctgcgaggca tctttcacca gccaggcgat
    18541 ggactccggg atcgcagcag tgacccacac cgggtccata ccctgaattt tcgcgaccac
    18601 gttatctttg gcgatattca ttcccacgcg cagatcaaac gcggtgatca cgccatcaat
    18661 gggcgctttg agcgtaaagc gagtctggat tttttgcgtg gcgatcaggc ggcgaatatc
    18721 agcctccggc attcccgcca gtcgcagtcg ctcaagaatg ccttcagtct gggtcgccgt
    18781 accgccggtt tcgcgcagca gtaaatactc actctgcgct tccacccagt cgggaatggt
    18841 caggtcgaga agcggcgcgc ccttttgcac tttatcgccc acggtaagcg gatacacctt
    18901 gtcgataaac ccggcggcgc gggcctgcac aatggcatac tgatactcgt tgtaactgac
    18961 atttgccggg aaactctggg caaaagtcag cggcccacgc gtgacggtcg ccgttttcac
    19021 ccccagattc tgcgtctgag tcgggtcaat gcgcacacca gatgcagaac tctcttcatc
    19081 ggcatatttc ggcaccagat ccatatccat aaacggcgat ttccctggtt tatcgaaccg
    19141 cgtattggga tacatcgggt cgtaccagaa taagacttta cgttctgcgg tcgacgtttt
    19201 ttctgcgggc agttcagcct ttgcaaacca ggtaaaacct gccgcagaaa taataccgcc
    19261 cgcgatcatg ctgccgataa taagcgcgat ttttttcatc tcattaaacc tgggttactg
    19321 gctgacttta atatcctgta ataaagaaag gctgccctgc tggacaaaat taaacgccac
    19381 tttgtcgccg gttttaattt cgctcatttt cgtctgcggg gtgatggtaa agcgcatggt
    19441 catctccggc cagttcacgg cagcaatcgg atcgtgatgg atggtgattt ttttgctttc
    19501 cagatcaaca ccttttacca caccagtggc gctaataacc tgtggttgtg cttcgctcat
    19561 ggtttcatga tgatgttcgt tagcctgggc attaaagcca ataacggtaa acagactaaa
    19621 cattgcgact tgcagtgctt ttttcattta ttctctcctg gagttaaaaa ttattgctgc
    19681 caaccgccgc ctaaggcggt atataaagaa atttcgttaa cctgacgggc ataattcagg
    19741 tcaagtaaag tttgtcgggt tgcaaataaa gaacgctcgg catccagcac ttccagataa
    19801 cttaccgcgc cgtgctgata taatgcccgc gcccgttgca aagtaatttg cagcgacgca
    19861 agataacgct gctgggcgct gatttgatcg ttcaggcttt gacgtaatgc cagcgcatct
    19921 gccacttctt taaaggcgtt ctggattttc tgttcataat tcaccaccga ctgctgctgg
    19981 cgaatttcgg cgatatccag attggcctgg ttgcgtccag cattaaaaat ggggatctca
    20041 attttaggaa taaaattcca catcccgctg ctggcattaa ataatgacga cagatcgctg
    20101 ctggcggttg atattccgct ggtcaggctg atagacggga aaaatgccgc acgcgcagca
    20161 ccaatattgg cattagccgc cattaacgcg tgttcagctt ccataatatc agggcgctgc
    20221 aataagattt gcgacggcaa gcccgccggt aatttaacgc tttgcaggct gtcgctgttt
    20281 actgtctgcg cttgcggcag cttgccgtag cttcccaata acagttgcaa tgcattattt
    20341 gcctgcgcca gttccccctg acgtttagcg atgtcgctgc gggtactttc tatcaccccg
    20401 cgagcctgtt ccagcgccag aacattgctg ctaccggtca acagttgttt ttcgacaaac
    20461 gcatatgact gctgataatt acgcagcgtt tcttcggcta tttgcaattg cgcatacgcc
    20521 agttgctgat tgaaatagct ttgcgcgaca ttagaaacca gcaaaatatg caccgcgcgc
    20581 tgagcttcct cagtggctaa ataattttgg cgctcggctt cgctcatgtt ctttaagcgc
    20641 ccgaaaaaat cgagatcaaa gctggcgtta aggccagtcg agaactcccg cgtcgtggct
    20701 gaatcgcctt taagattgcc gctccagctg ccgctgccct cgccattgag ctgtgggtag
    20761 cggtcggcat cggtcagacg atattgcgcc cgcgcttcct gcactttcag cgccgccatg
    20821 cgcaaatccc ggttattcac caacgcctcg ctaatcagcg tcttcacctg attatcaaca
    20881 aaaaaggtgc gccagcccgc gttctggtag ttatctgctg cgttaaccag gccgttctgg
    20941 ctgagtgaga actgctgcgg cacgggcatt gccggacgct gataatccgg tgccagtgaa
    21001 caaccggtta gcgcaagggc cacacaaaat ggcagaagtt tacaaggaga cataggctca
    21061 taatttctgg tgattttatg ctgccaactt tactcgccag gctctgattt tccggtgaca
    21121 ggaaaatgac aaaattgtca ttttgccaat aagcgattgc catctgatcc cgctactcta
    21181 gaattgcccg ggcaacatgc ggaggaaata tgaaactgtt gattgtcgaa gatgaaaaga
    21241 aaaccggaga atacttgacc aaagggttaa ccgaagccgg ttttgtggtc gatttggccg
    21301 acaacgggct gaatggctac catctggcga tgaccggtga ttatgatctg ataatcctcg
    21361 atattatgct gccagacgtg aacggctggg atatcgtgcg catgttgcgt tccgccaata
    21421 aggggatgcc gattctgttg cttaccgcgc ttggcaccat tgaacatcgc gtcaaggggc
    21481 tggagttggg ggcggatgac tatctggtga agccgttcgc ttttgctgaa ctgctggcgc
    21541 gggtgcgcac tctactgcgg cgcggggcgg cggtgattat cgaaagtcag tttcaggttg
    21601 ccgatctgat ggtcgatctc gtcagccgca aagtcacccg cagcggcacg cgcatcactc
    21661 tgaccagtaa agaatttact ctgctggagt ttttcctccg ccatcagggc gaagtgctgc
    21721 cccgctcgct tatcgcctcg caggtatggg acatgaattt tgacagcgat accaacgcca
    21781 ttgatgtggc ggtgaagcgg ctgcgcggca aaatcgacaa cgactttgag ccaaagttga
    21841 ttcagaccgt gcgcggcgtg ggttacatgc tagaggtgcc ggatggtcag taagccattt
    21901 cagcgcccgt tttcgctggc aacccgcctg acctttttta tcagcctcgc caccatcgcg
    21961 gcgtttttcg cctttgcatg gatcatgatc cactcggtaa aagtgcattt tgccgagcag
    22021 gatattaatg atttaaagga gattagcgcc acccttgaac gggtacttaa tcaccctgac
    22081 gaaacgcaag cccgacgctt aatgacgctg gaagatatcg tcagtggtta ttccaatgtg
    22141 ttgctctccc tggcaaatag ccacggtaaa acggtgtatc actccccagg tgcgccggat
    22201 atccgcgagt ttacgcgtga cgccataccc gataaagacg cgcaggctgg cgaggtgtat
    22261 ctcctttccg gcccgacgat gatgatgcca gggcacggtc acgggcatat ggaacacagc
    22321 aactggcgga tgattaactt gccggttggc ccgctggttg acggcaaacc gatttatacg
    22381 ctctacatcg cgctttcgat cgattttcat cttcattaca tcaatgattt gatgaataaa
    22441 cttattatga ccgcatcggt aatcagcatc ctgatcgtct ttatcgtgct gttggcggta
    22501 cataaaggtc acgcgccgat ccgcagcgtc agccgtcaaa tccagaatat cacctcgaaa
    22561 gatctcgacg ttcgccttga cccgcagacc gtgcccattg agctggaaca gctggtactg
    22621 tcgttcaacc atatgatcga gcgtattgag gatgtcttta cccgccagtc caatttctca
    22681 gcggatatcg cccacgaaat tcgcacacct attactaatc tcattacgca aacggaaatc
    22741 gccctcagcc agtcgcgcag ccagaaggag ctggaagatg tgctctactc taatctcgaa
    22801 gagctgacgc gaatggcgaa aatggtcagc gatatgctgt ttctcgctca ggccgataac
    22861 aaccagctaa tccccgaaaa gaaaatgctc aacctggggg atgaagtcgg caaagtgttc
    22921 gattttttcg aggcgttagc agaagatcgc ggcgtggagc tgcggtttgt tggcgacgag
    22981 tgtcaggttg cgggcgatcc gctgatgctg cgtcgggcgt taagtaacct gctttctaat
    23041 gccctgcgtt atacgccaac cggagagaca attgttgtgc gctgccagac ggtcgatcac
    23101 ctggtgcaag ttaccgtcga aaaccccggt acgcccattg cgcccgaaca cttaccgcga
    23161 ttgtttgacc gtttctaccg cgttgatcct tcccgccagc gaaaaggcga aggtagcggc
    23221 attggcctgg cgatagtgaa atcgattgtt gtcgcgcata aaggcacagt tgcagtaacg
    23281 tcagatgtgc gggggacaag gtttgttatc atattacccg cttaatcttt cagcaaacgg
    23341 caacttttat aaccagtgta aaaataacat gccgcaatag taatacctat taatgataaa
    23401 aagctgtcac aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa
    23461 tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa
    23521 aagtaatcgc gataacgtta gcggtcatta tgttcatcag cgggctggac gattttttta
    23581 ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc
    23641 cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc
    23701 cggcgtggaa tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg
    23761 actacgaaaa ctatcatatc tttgttggca cctaccccaa cgaccccgat actcagcgtg
    23821 atgttgacga agtgtgcgct cgcttcccga acgtgcataa ggtagtctgc gcgcgtcctg
    23881 gccccaccag caaagccgac tgtctgaaca acgtgctgga tgccatcacc caatttgaac
    23941 gtagcgccaa tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac
    24001 cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccag
    24061 tgtatccgtt cgaacgcgaa tggacgcact tcaccagcat gacctacatt gatgagtttt
    24121 cagaactgca tggcaaagat gttccggtgc gtgaagccct cgccggacag gtgcccagcg
    24181 caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc actgttagct gacggtgacg
    24241 gtattgcttt cgacgtgcag agtctgactg aagattacga cattggcttc cgcctgaaag
    24301 agaaaggtat gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg
    24361 agcagcgtaa atttttacag catgcacgga cgtcaaatat gatctgcgtg cgcgaatatt
    24421 tccccgatac cttttcgact gcggttcggc aaaaatctcg ctggatcatc ggcattgttt
    24481 tccagggctt taaaacccac aaatggacct ccagcctgac gctgaactac tttctctggc
    24541 gcgaccgcaa aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatac
    24601 agcttttgct gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta
    24661 ttttcagcgg cagcgcatgg ttaatgactc tgctgtggct aaactttggc ttgatgatta
    24721 accgcatcgt gcagcgggtg attttcgtta ctggctacta cggcctgacg caggggctgc
    24781 tatctgtcct gcgtcttttc tggggcaacc tgattaactt catggccaac tggcgcgcgt
    24841 taaaacaggt acttcaacac ggcgatccac gtcgcgtcgc gtgggataaa acaacgcatg
    24901 acttccccag cgtgactggc gatacccgct cgttgcgtcc gttaggtcaa atcctgctgg
    24961 aaaatcaggt catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc
    25021 tacgcctggg cggttcaatg ctgatgcagg ggctgattag cgccgagcaa ctggcacagg
    25081 cgctggcaga gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccttcct
    25141 cgctgattgc cgaaatgccg gcctccgtgg cgctgcatta tgcggtactg ccgctgcgtc
    25201 tggacaatga tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg
    25261 ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg
    25321 tcaccggatt acgccactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt
    25381 acaatgcagt tcagcatcag tggctcacgg aacagcaggc cggtgaaatc tggcggcaat
    25441 atgtgccgca tcagttcctg ttcgcagaaa tactgaccac gctcggtcat attaatcgtt
    25501 cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt
    25561 tggtcaccga aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg
    25621 aattacaagt ttcgatgcaa tcactattac tcaaagcagg tttaaacact gaacaggttg
    25681 cacaactgga gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt
    25741 ttactgctga catctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca
    25801 gaagagctgg ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg
    25861 ctgaaggcac agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac
    25921 cagcaggtgc cggataatat tccgctgact ttataccttg cggaagccta tcgccatttt
    25981 ggtcatgatg accgggcgcg gctgttgctt gaggatcaac tgaaacgtca ccctggagat
    26041 gatcggcttg agcgcagtat ggcggctatt cctgttgaag tgaaacgcgt tacgactgtt
    26101 gaagaactgc ttgcccagca aaaagcgtgc gatgctgcgc cgaccctgcg ttgtcgcagt
    26161 gaagtcgggc agaatgccct gcggctggca caattacctg tcgccagagc gcaactgaac
    26221 gatgcgacgt ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgc
    26281 gcaatctacc tgaaacaatg gtcccaggca gatgcgctat acaatgaagc acgccagcag
    26341 aacacattaa gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagttg
    26401 gacgatcgga tcctggcgct gcaatcacag gggatcttca ccgatcctca gtcatatatt
    26461 acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt
    26521 gaaaatacgc cgctatttac cacggacgca caagagaaaa gttggctcta tctgttatct
    26581 aaatacagcg gcaaccccgt tcaggcgttg gcgaattata cggtacagtt tgccgacaac
    26641 cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacggcgca
    26701 gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttataccgtt
    26761 agcgtagcga cccataacaa ggctgaagct ctgcgtctgg cacgattgct gtatcagcaa
    26821 gaaccggcaa atcttacccg cctggatcaa ctgacctggc aactgatgca gaacgaacag
    26881 tcacgcgaag ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtgtc
    26941 agccagactt taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctggcaacg
    27001 ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa
    27061 agtcagttgc cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg
    27121 tcgccttcct acgatgccgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta
    27181 cccggtgtgg cgttgtatgc atggcttcag gccgaacaac gacaaccgga cgcctggcaa
    27241 catcgtgcgg tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc
    27301 tggcagaaaa tcagtcttca cgacatgagc aatgaggatc tgcttgctgc tgccaatacc
    27361 gcccaggcgg caggaaatgg tgcggctcgc gatcgctggc ttcagcaggc agaacaacgt
    27421 gggctgggaa acaatgccct ctactggtgg ctgcatgcgc aacgttacat tcctggtcag
    27481 ccggaacttg cactgaacga tctcacgcgc tcaatcaata ttgcgccttc tgccaacgct
    27541 tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg tcccggcggc ggtgagtgat
    27601 ttgcgcgccg cgctggaact ggaaccgaat aatagcaaca cccaggcagc gctcggttac
    27661 gccttgtggg atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa
    27721 gggctaccgg acgatccggc acttatccga caactggcct acgtgaacca gcgtctggat
    27781 gacatgcctg cgacgcagca ctacgcccgg ctggtgattg atgacattga taatcaggcg
    27841 ctgataaccc cactgacccc agagcaaaat cagcaacgct tcaatttccg ccgtctgcat
    27901 gaggaggtcg gtcgccgctg gacgttcagt ttcgattctt ctatcggctt gcgttccagc
    27961 gcaatgagta ccgctaacaa taatgtcggc ggcgcagcgc cagggaaaag ctatcgtagc
    28021 tacgggcaac tggaagccga gtaccgcctc ggacgcaata tgctgctgga aggcgacctg
    28081 ctctcagttt atagccgcgt ctttgccgat accggagaaa acggggtgat gatgccggtg
    28141 aaaaatccga tgtccggcac cggtctgcgc tggaagccgc tgcgcgatca gatctttttc
    28201 ctcgccgtcg aacagcagtt gccgctgaac gggcaaaatg gcgcatccga taccatgctg
    28261 cgcgccagcg cctcattctt taatggcggc aaatacagcg acgaatggca cccgaacggt
    28321 tcaggctggt ttgcccaaaa cctgtacctc gatgcggcgc aatatgtccg ccaggatatt
    28381 caggcgtgga cggcagatta tcgcgtaagc tggcatcaga aggtggcgaa cggacagact
    28441 attgagcctt acgctcacgt tcaggacaac ggttatcgtg ataaaggcac tcagggcgcg
    28501 cagcttggcg gtgtcggggt ccgctggaat atctggaccg gcgagacgca ctacgacgcc
    28561 tggccgcaca aagtcagtct cggcgtcgaa tatcaacata cctttaaggc gattaatcaa
    28621 cgtaacggag agcgcaacaa cgcgtttctc accattggag tgcactggta aatgcgtaag
    28681 ttcattttcg tattgttgac actgcttttg gtcagccctt tttcctttgc gatgaaaggt
    28741 attatctggc aaccacaaaa ccgtgatagt caggttaccg atacccagtg gcaggggctg
    28801 atgagtcagt tacgtttgca aggcttcgat accctggttt tgcaatggac ccgttacggc
    28861 gatgcattca cccagccaga acagcgcgcg ttattgttta agcgtgctac agctgcgcaa
    28921 caggctggcc tgaagctgat tgtcgggctg aacgccgatc cggaattttt tatgcaccag
    28981 aaacagtcgt ccgcagcgct ggaaagctat cttaatcgcc tgctggctgc cgatctccag
    29041 caagccagat tatggagcgc cgtgcctggc gtaacgccgg atggctggta catcagcgcg
    29101 gaaattgacg acctgaactg gcgcagcgaa gccgcccgtc agcctttgct aacatggtta
    29161 aacaacgcgc agcggttgat tagcgatgtt tcagcaaaac cggtttatat cagtagtttt
    29221 ttcgccggaa acatgtcgcc agatggctat cgccaactgc tggaacaagt taaagcaacc
    29281 ggtgttaatg tctgggtaca ggatggcagc ggcgtggata aactgaccgc tgaacagcgt
    29341 gaacgttatc tacaggccag cgccgattgc caaagttccg ccccagcaag cggcattgtt
    29401 tatgaacttt ttatcgccgg caaaggcaaa acctttacag cgaaaccgaa accggacgca
    29461 gaaattgcct cgctgttagc gaaacgttcc tcttgcggta aagacacgct ctatttctct
    29521 ctgcgctatt tgcccgccgc gcagagtatc cttgagtatt aatgattaaa gggagcaact
    29581 tagcttgctc ctttcctttt cactttccta atcaaaatcc tggcaaggtg aattgtttag
    29641 cagaatttct gttttcattt tattcgtctg acgtgttcca attatttagc aaaaccgcga
    29701 caacaaagtg atggtgtaat ttgtaaaagg gaacaaataa atggctcgcc ctgcaggatt
    29761 cgaacctata gcccacggct tagaaactcc aggaacgaca ttttaagtca acaaattacc
    29821 gcgccatctc tgcgttcaca cgtcccacca cctcaaaata tgtaaaacct tgcaagccat
    29881 tgcgaggcct tatgtgtctc agttttgttc ctctttttta tactaaaaaa cacagcaatt
    29941 gaggataaac ctcatgctat tatcgctaat atgcatctaa aggcatggca cttgaataga
    30001 taaaaactcc acaaagaaaa aaataattag cccataaaac cacaatacaa aacacaaaca
    30061 gacaattaaa ctaaaaaact gacagcgcat tgtgacaaaa atccaatg
//
LOCUS       DADTFG010000047        29972 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.047, whole genome shotgun sequence.
ACCESSION   DADTFG010000047 DADTFG010000000
VERSION     DADTFG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.047"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            732..1820
                     /locus_tag="J1138_001940"
     CDS             732..1820
                     /locus_tag="J1138_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBA8453743.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            1831..4110
                     /locus_tag="J1138_001941"
     CDS             1831..4110
                     /locus_tag="J1138_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453744.1"
                     /translation="MSEPLIVGIRHHSPACARLAKSLIESQRPRYVLIEGPADFNDRI
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRDTRMDNSDTLWDHLFEDESQQTALPSALARYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALANMWRECPQEIN
                     KPELSSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILVTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQMTLCAAIDRTLWLCESNGRPDEK
                     EFHTHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            4103..5239
                     /locus_tag="J1138_001942"
     CDS             4103..5239
                     /locus_tag="J1138_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA8453745.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSNATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            5236..7239
                     /locus_tag="J1138_001943"
     CDS             5236..7239
                     /locus_tag="J1138_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453746.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLFATRNRLSGVVPLSPDAWLMLSAPLRQPGIVAL
                     REYLRQRPPACIRPINQVDNLFILPIAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            7364..7825
                     /locus_tag="J1138_001944"
     CDS             7364..7825
                     /locus_tag="J1138_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBA8453747.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(7866..8336)
                     /locus_tag="J1138_001945"
     CDS             complement(7866..8336)
                     /locus_tag="J1138_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBA8453748.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(8383..9102)
                     /gene="yehT"
                     /locus_tag="J1138_001946"
     CDS             complement(8383..9102)
                     /gene="yehT"
                     /locus_tag="J1138_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBA8453749.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(9099..10784)
                     /gene="btsS"
                     /locus_tag="J1138_001947"
     CDS             complement(9099..10784)
                     /gene="btsS"
                     /locus_tag="J1138_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBA8453750.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            11006..11737
                     /gene="mlrA"
                     /locus_tag="J1138_001948"
     CDS             11006..11737
                     /gene="mlrA"
                     /locus_tag="J1138_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBA8453751.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQATLQALLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            11797..11904
                     /locus_tag="J1138_001949"
     CDS             11797..11904
                     /locus_tag="J1138_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBA8453752.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(11885..12616)
                     /gene="yehW"
                     /locus_tag="J1138_001950"
     CDS             complement(11885..12616)
                     /gene="yehW"
                     /locus_tag="J1138_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBA8453753.1"
                     /translation="MKMLRDQLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAGFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(12621..13547)
                     /gene="yehX"
                     /locus_tag="J1138_001951"
     CDS             complement(12621..13547)
                     /gene="yehX"
                     /locus_tag="J1138_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBA8453754.1"
                     /translation="MIEFSHVSKLFGTQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIASVPQL
                     QKWSRARIDDRIDELMALLGLEPDLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            complement(13540..14697)
                     /gene="yehY"
                     /locus_tag="J1138_001952"
     CDS             complement(13540..14697)
                     /gene="yehY"
                     /locus_tag="J1138_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBA8453755.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIVPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGTQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(14704..15621)
                     /gene="osmF"
                     /locus_tag="J1138_001953"
     CDS             complement(14704..15621)
                     /gene="osmF"
                     /locus_tag="J1138_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBA8453756.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQIAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(15811..18108)
                     /gene="bglX"
                     /locus_tag="J1138_001954"
     CDS             complement(15811..18108)
                     /gene="bglX"
                     /locus_tag="J1138_001954"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBA8453757.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMLPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            18304..20019
                     /gene="dld"
                     /locus_tag="J1138_001955"
     CDS             18304..20019
                     /gene="dld"
                     /locus_tag="J1138_001955"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBA8453758.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVSADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEANTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     VLKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVK"
     gene            complement(20058..20990)
                     /gene="pbpG"
                     /locus_tag="J1138_001956"
     CDS             complement(20058..20990)
                     /gene="pbpG"
                     /locus_tag="J1138_001956"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBA8453759.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLAMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(21164..21751)
                     /locus_tag="J1138_001957"
     CDS             complement(21164..21751)
                     /locus_tag="J1138_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBA8453760.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            21921..22499
                     /locus_tag="J1138_001958"
     CDS             21921..22499
                     /locus_tag="J1138_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA8453761.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWSLIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(22619..23380)
                     /locus_tag="J1138_001959"
     CDS             complement(22619..23380)
                     /locus_tag="J1138_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA8453762.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(23433..24959)
                     /gene="mdtQ"
                     /locus_tag="J1138_001960"
     CDS             complement(23433..24959)
                     /gene="mdtQ"
                     /locus_tag="J1138_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBA8453763.1"
                     /translation="MPLMYCHAKLKNISQHTVISAHLFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQHTPAVQVDTALPTALKNGWPDSQWWLEYHDDQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQLDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINASKTRQQ
                     LNDVAGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGLIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            25093..25176
                     /gene="yohP"
                     /locus_tag="J1138_001961"
     CDS             25093..25176
                     /gene="yohP"
                     /locus_tag="J1138_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBA8453764.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(25564..26514)
                     /gene="dusC"
                     /locus_tag="J1138_001962"
     CDS             complement(25564..26514)
                     /gene="dusC"
                     /locus_tag="J1138_001962"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBA8453765.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            26753..27151
                     /locus_tag="J1138_001963"
     CDS             26753..27151
                     /locus_tag="J1138_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBA8453766.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            27148..27843
                     /locus_tag="J1138_001964"
     CDS             27148..27843
                     /locus_tag="J1138_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBA8453767.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            27973..28857
                     /gene="cdd"
                     /locus_tag="J1138_001965"
     CDS             27973..28857
                     /gene="cdd"
                     /locus_tag="J1138_001965"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBA8453768.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            29007..29726
                     /gene="sanA"
                     /locus_tag="J1138_001966"
     CDS             29007..29726
                     /gene="sanA"
                     /locus_tag="J1138_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBA8453769.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            29729..29968
                     /locus_tag="J1138_001967"
     CDS             29729..29968
                     /locus_tag="J1138_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBA8453770.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
BASE COUNT         7109 a         8070 c         7642 g         7151 t
ORIGIN      
        1 cccaggagct tactaaagta agtgacaggg gtgaacgaca aatccgcatg gagcggattt
       61 gaacgctact tgtagcggcc ccgtaagggg tgaggtccat ggatgggccg aataacccgc
      121 agtcgcagca catgcaactt gaagtatgac aagtaaagct ttattaaagg gggaaacggt
      181 attccccctt aaatgcgttt caatttgccc gttacttcaa caaatcaatc aaccgtaaca
      241 gagccactaa taactccagt acggcaatga gcacggttaa cttttgtgtc agcgtgtcca
      301 ttcgctctcc ttattaagga gcgcaacacc agcatcttac ccttacactg cctgtcgata
      361 ctggtttacg tatgttgagt cgccatgttg cactccggat cagagcacta cctgcggcac
      421 cacccgtccg gtagccagga cttgaaattg gttcgatccg gcgcaacttc ccaacagtca
      481 ggattataaa tctcattatc ttaattgcaa cggggtccgt aggtggttat aattcgttcg
      541 ttggggcgcc atgttgcata atcagtaact taacgtcaaa gttgtaaata aaagaacctg
      601 cctcaccagc aggttttttt attttttgcg atctgctttc cagatatttt ccgctcaaac
      661 aactaatgcg ccaaacattt attgcgcgta aaatatcgtt tatttcatta atacatttca
      721 gggatgaata tatgtcaccg cagaataatc acctgcagcg tccgcctgct gctgtgttat
      781 acgccgttga actggcaaaa ttaaaacaaa atgataacgc accttgcccg ccaggttggc
      841 agttaagttt gcctgcggcc cgtgctttta tccttggcga cgaagcgcaa aatattagcc
      901 gtaaagtggt gattagtccc tccgctgtcg aacgtatgtt agtcactctt gccaccgggc
      961 gcggtttgat gttggtgggg gaacctggca ccgcaaaatc tctcctgtct gaattactgg
     1021 caaccgctat tagcggcgat gccgggttaa ccatccaggg cggcgcatct actactgaag
     1081 atcaaatcaa gtacggctgg aactacgccc tgcttatcaa ccatggtccg tcaacggaag
     1141 cactggtccc tgccccgctt taccagggaa tgcgcgacgg caaaatcgtc cgttttgaag
     1201 agataacgcg tacaccgctg gaagtacaag actgtctgtt aggtatgctt tctgacaggg
     1261 tgatgacggt gccggaactc accggtgaag ccagtcagct ctatgcgcgg gaaggcttta
     1321 atattattgc cactgccaat acccgcgatc gcggcgtcaa tgaaatgagt gccgcgctaa
     1381 agcgtcgctt cgattttgaa actgtctttc cgattatgga tttcgcccag gagctggagc
     1441 ttgtcgccag tgcttcggcg cgtttgctgg cccatagcgg cattccgcat aaagtgcctg
     1501 acgcggtact ggagttactg gtccgcacct ttcgcgatct gcgtgccaac ggcgaaaaga
     1561 aaacgtcaat ggatacactg acagcgatta tgtccaccgc tgaagccgtg aacgtggccc
     1621 acgcggtggg cgtccgcgcc tggtttttag cgaatcgcgc aggtgagccg gccgatctgg
     1681 tggactgtat tgccggaacc atcgtcaaag ataacgaaga agatcgcgcg cgcctgcgcc
     1741 gttacttcga acaacgtgtt gctacacata aagaagctca ctggcaggct tattatcaag
     1801 ctcgccaccg cctgccgtga ggaaagatgt atgagcgagc cgttaattgt cggcatccgg
     1861 catcatagtc cggcctgcgc ccggctggcg aaatcgttaa tcgaaagcca gcggccacga
     1921 tacgtattga ttgaaggccc ggctgatttt aatgaccgga tagacgaact gtttttagcc
     1981 caccagcttc cggtagctat ttacagttat tgccagtatc aggacggtgc ggcccccggg
     2041 cgtggtgcct ggacgccatt tgctgaattt tcgccggagt ggcaggcgct acaagccgca
     2101 cgtcgtattc aggcacaaac ttacttcatc gatttgcctt gctgggcgca gagtgaagaa
     2161 gaggacgatt cgcctgatac acaagatgaa agccagacct tactgctgcg tgacacccgc
     2221 atggataaca gcgataccct gtgggatcac ttattcgaag atgaaagcca gcaaactgca
     2281 ttaccctctg cactggcgcg ctattttgcc caactgcggg gcgattcccc cggagatgct
     2341 ctcaatcgtc agcgcgaagc ctttatggct cgctggatta catgggcgat gcagcaaaac
     2401 aatggcgacg tgttagtcgt ctgcggcggc tggcacgctc ctgcactggc aaacatgtgg
     2461 cgcgaatgcc cgcaggaaat taacaagcca gaattgtcct cgctggcaga tgccgttaca
     2521 ggttgttatc tcacacccta cagtgaaaag cgccttgatg tgctggcagg atacctttcc
     2581 ggaatgcctg ccccggtctg gcaaaactgg tgctggcagt gtggcttaca gcaggcaggt
     2641 gaacaactgc tgaaaacggt tcttacccgt ttgcgccagc acaaattgcc cgcttctacc
     2701 gctgatatgg ctgctgctca tctgcatgct atggcactgg cacagttgcg cggccataca
     2761 ctacctctac gcactgactg gctggatgcc atagctggct cgctgattaa agaagccttg
     2821 aacgcgccgt tgccgtggag ctaccgcgga gttattcatc ccgataccga tccgattctg
     2881 gtaacgttga tagacacatt agcgggtgac ggattcggta aacttgcccc ttctacgcca
     2941 caaccgcctc tgccaaaaga tgtcacctgc gaactggaac gtaccggaat ctctcttccg
     3001 gcggagctta ccttaaatcg ctttaccccc gatggactgg cgcaaagtca ggtgttacat
     3061 cggttggcaa tactggagat cccaggggtt gtgcgccagc agggaagtac gctgacactt
     3121 gcaggcaacg gtgaagaatg ctggaaatta acccgcccgc ttagccagca tgctgcattg
     3181 attgaggccg cctgctttgg tgccacactc caggaagccg cacgccataa attagaagcc
     3241 gatatgctgg acgcgggtgg aatcggctgt atcaccacat gtcttagcca ggcagcgtta
     3301 gcgggtctgg cgtctttcag tcaacaatta ctggaacaac tcacattatt aatcgcccag
     3361 gaaaatcaat tcgccgaaat gggccaggca ctggaagtgc tatatgcctt atggcggttg
     3421 gatgaaatta gcggtatgca aggcgcgcag atattacaaa tgacattatg cgcggccatc
     3481 gatcgcacgc tgtggctatg tgaatccaac ggcagaccgg atgaaaagga gtttcacact
     3541 cacctgcata gctggcaggc gctttgccat atcctgcgcg atctacatag cggcgttaat
     3601 ttacccggcg tttctctttc tgcggcggta gccttactgg agcgccgcag tcaggcgatt
     3661 catgccccgg cgctggatcg cggcgcgact cttggcgcac tgatgcgtct ggaacatccc
     3721 aacgccagtg ccgaagcggc gctgacgatg ctggcgcagt tatccccggc acaatccggc
     3781 gaggcgctgc acggtttgct ggcattagcc cgtcatcaac tggcctgtca gccggtattt
     3841 atcgccggtt tcagcagtca tttaaatcaa ctgagtgatg ccgattttat caatgccctg
     3901 cccgatttac gcgcggcgat ggcctggcta ccacctcgag aacgcgggac gctggcgcat
     3961 caggtgcttg agcattatca actggtgcaa cttcccgttt cggcactgca aatgccgttg
     4021 cattgtccac cacaagacat tgcacatcat caacaactcg aacagcaggc actggcatcg
     4081 ctgcaacact ggggagtttt ccatgtctga actgaacgat cttctgacca cccgtgagct
     4141 acaacgctgg cgattaattc ttggcgaagc ggcagaaacg acgctttgtg ggctggatga
     4201 caacgcccgg cagatagacc acgcgctgga gtggctgtat gggcgcgatc ctgaacggct
     4261 ccagcgtggt gaacgttacg gtggattagg tcgttcaaat ctcaccaccc ctgagtggat
     4321 caacagcatt cacacgctgt ttccgcaaca ggtgattgag cggctggaaa gcgatgccgt
     4381 gctgcgctac ggcattgaag atgtggtgac gaatctcgac gtgctggaac gtatgcagcc
     4441 ttctgaaagc ctgctacgcg ccgttttgca caccaaacat ctgatgaatc ccgaagtact
     4501 ggctgccgcc cgccggatag tgcaccaggt tgttgaagag attatggcgc gactggcaaa
     4561 ggaagttcgt caggcttttt ctggagtacg cgatcgccgt cgccgctcat ttatttcact
     4621 ggcgcgaaac tttgatttca aaagtactct gcgcgccaac ctgcaacact ggcacccgca
     4681 acacggcaag ttgtatatcg aatccccccg ctttaacagc cgcattaaac gccatagcga
     4741 acaatggcaa ctggtcctac tggttgatca aagcggatcg atggttgatt cggtgatcca
     4801 ctccgcagtg atggcggctt gtttatggca gttacccggc attcgtaccc atctggtcgc
     4861 ttttgacacc agcgtggttg atctcacggc agacgttgcc gatccggtag agttattaat
     4921 gaaagtacaa ctgggcggcg ggaccaatat cgccagtgcc gtggagtatg gtcggcaact
     4981 tattgaacaa cctgcaaaaa gtgtcattat tctcgtgagc gatttttatg aaggtgggtc
     5041 atcatcattg ctgacgcatc aggtgaaaaa gtgtgtccag agcggcatca aagtgctggg
     5101 gctggcagcg ctcgacagca acgcaacgcc ttgctatgac cacgatacgg cccaggcgct
     5161 ggttaatgtc ggcgcacaaa tagccgcaat gacaccgggc gagctggctt cctggcttgc
     5221 ggagaatctt cagtcatgaa ttcactacgt ccggaattat tagaactgac accacaggca
     5281 ctgacggcgt taagcaatgc cggttttgtt aagcgcagcc ttaaggaact ggaaaatggc
     5341 aacgtcccgg aaatcagtca tgagaacggc gctttaatcg ccaccttcag tgacggtgtc
     5401 cgtacccagc tggcgaacgg ccaggcactg aaagaggctc agtgcacctg cggggccagc
     5461 ggcatgtgcc gtcatcgcgt gatgctggtg ttaagttatc aacgactttg tgccaccgcc
     5521 cagcctacgg aaaaaaaaga agaagagtgg gatccggcaa tctggctgaa agaactggct
     5581 accttgcccg atgccacccg caaacgcgcg caggcgctgg tcgctaaagg catcaccatt
     5641 gagttgttct gtgcgcctgg cgaaattccc tctgcccgat taccgatgag cgatgtgcgt
     5701 ttttattccc gcagcagcat tcgcttcgca cgctgtgatt gcattgaagg cacactttgc
     5761 gaacatgtcg tactggctgt gcaggccttc gtcgaggcca aaactcagca agcggaattt
     5821 actcatttaa tctggcagat gcgcagtgaa cacgtcacat catctgatga tccgtttgcc
     5881 agcgaagaag gcaaaacgtg tcgtcaatat gttcagcaat taagccaggc attatggctg
     5941 ggcgggataa gtcaaccgcc aatccattac gaggccgctt tcagtcgcgc gcagcaagcg
     6001 gcggaacgct gcaactggcg atgggtgagt gaatcgctac ggcaattgcg cgccagtgtt
     6061 gatgccttcc acgcccgtgc cagccactac catgccggag aatgcttacg tcagcttgcg
     6121 gcattaaaca gtcgattaaa ttgcgtacaa gagatggcgc ggcgcgacag tatcggtgaa
     6181 gttcctccca tgccgtggcg cacggtcgtt ggcgctggta ttgccggaga agcaaaactt
     6241 gatcatctgc ggctggtgtc tttaggtatg cgttgctggc aggatattga gcagtatggt
     6301 ttacgcatct ggtttaccga tcccgacaca ggaagtattt tgcatctttc gcgtagttgg
     6361 cagagaagcg aacaggaaaa ctcaccggca gcgacgcgtc ggctgtttag ttttcaggct
     6421 ggcgcactgg caggtgggca aattgtttca caagctgcaa aacgcagtgc cgatggcgag
     6481 ctgctgttcg cgacccgtaa ccgcttgagt ggcgttgttc cactgtcgcc agacgcctgg
     6541 ctaatgttga gcgcgccgtt gcgccagccg ggcattgtgg ctttgcggga atatttacgc
     6601 cagcgtcccc ccgcctgcat acgaccaatc aaccaggtcg ataacttatt tattctgccg
     6661 atagcggagt gtatttcgct cggttgggat agcagccgcc agacactgga tgcgcaggta
     6721 atcagcggcg aaggggaaga taatgtgctg acgttatccc ttccggcatc tgcctgttct
     6781 ccttttgccg ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgagcctg
     6841 gtttctggct ttgtcagttt tgtagagggg caattgacac tggaaccacg ggtgatgatg
     6901 acaaaaaccc gcgcctgggc gctggacgca gaaactgcgc ctgtggcacc gctaccttct
     6961 gccagcgttc tgcctgtgcc gtctaccgct catcagttgc tgatgcgctg ccaggcgtta
     7021 cttattcaac tgctccataa cggctggcgc tatcaggaac agagtgctat tggtcaggca
     7081 gagttgctgg cgaatgacct caccgcggtg ggtttttatc ggctggcaca tgtgttggga
     7141 caatttcgta atacagaaag cgaggcacgg gtagaagcaa tgaataacgg tgttttgctt
     7201 tgcgaacaat tattccccat gcttcagcaa caaggatgaa atagtgcttt ttattaagag
     7261 ttctactcct gttccggact gctcacgcca cagtattagg catatcctat atagcccctg
     7321 gtgagagtca ccagttcctt gattaaataa aatggagttt tacatgaagg ctttcaataa
     7381 gctgttttcc cttgttgttg catctgttct ggttttctct cttgctggct gcggtgacaa
     7441 agaagaatca aagaaattca gcgccaatct gaacggcact gaaattgcca ttacctatgt
     7501 ctacaaaggt gacaaggtgc ttaagcaatc ttctgaaacc aaaattcaat ttgcctccat
     7561 tggtgcaacc accaaagaag atgccgccag gacacttgag ccgttaagcg ctaaatacaa
     7621 aaacatcgcg ggtgttgaag aaaaattaac ctataccgat acctacgcgc aggaaaacgt
     7681 gactattgat atggaaaaag tggattttaa agccctgcag ggtatttcag gaatcaacgt
     7741 ttctgctgaa gatgccaaaa aaggtatcac tatggcgcaa atggaactgg tgatgaaagc
     7801 cgctggtttt aaagaagtga aataatctgt cggcggccat gtttcgcatg gccgccatac
     7861 ccgtcttagc ttttcttcac atgctggcgc gctgccagtc cacgcagaaa ataacgtaaa
     7921 aattgatcgc cgcattcgcg gaagttttta tgatccggtg cacgcatcat cgccgtaatt
     7981 tccggcatcg aaacgcggaa ctgctgttcg gtgaggattg ccagaatgtc atcggtttta
     8041 agcgaaaacg cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg acgctccggc
     8101 tccagtgccg gagcagactc atccttgccg cgtttttcat aaatcaggcc attgaggaat
     8161 gacgacaaaa caatgtccgg acaacgctga aaaccctctt cgtcttcttt acgtagccag
     8221 acggcgatct gttccgcggt ggcttcgaca ttacccagcg ccagaatacg caccaggtca
     8281 ttattattgg ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact tagcatgagg
     8341 ccttcaggtg ttgatgaggc aaaaagccat tttagcagtc ttttacaggc caatcgcctc
     8401 ttttaagctt ttcagataac ggcggctgac cggcacggtt aagccattac gcaaaatcaa
     8461 ctcggcctgg ccgttatctt ccagacggat ctcctgtaaa tgcgcgaggt taaccagata
     8521 ctgacgatgg cagcgcagta gtggtgtacg actttccagg gtacgtaatg tcagttcggt
     8581 aaagccctct ttcccttcgt gactggtaac gtagacaccg ctcatccgac tgctgacaaa
     8641 tgccacatct ttcatttgca gcaaataaat ccgactatgc cccgtacaag ggataaattt
     8701 cagcgcctgt tgattttccg gtaacaacga aacatcctgc ttgctgcgct cctgacgcaa
     8761 tcgcgccagc gttttctcca gtcgcgcttc atcaattggc ttcagcagat aatcaaaggc
     8821 atgttcttca aaggctttaa tggcgtattc gtcaaacgcg gtgagaaaaa caatatacgg
     8881 gcgatgttcc gggtcgagca tccccaccat ttccagacca ctgatgcgcg gcatctggat
     8941 atcgagaaac agcacatccg ggcgcagttt atgcaccgcg ccgatccctt ctacggcgtt
     9001 tgaacactct ccaacgattt caatatcgct ctgctcctgc aaaaatacgc gcaggttctc
     9061 ccgtgcaagc ggttcatcat cgacaattaa gactttaatc atgcctcgtc cctccatggt
     9121 agtcgtaacg ttattcgggt gtaactatca ggctcacagg cgacgcttat cccatagtca
     9181 tcgccaaacc gttcacgtaa acgcttatcc accagattca tccccagccc actggcattg
     9241 gttaccggtt gatacaaacc ggcattgtct tcgatctcca gcatcaaatg ttgcccctca
     9301 cgtcgggcgc tgattgccac tcgccctgta tccaacagtt gtgatgtccc atgtttaatg
     9361 gcgttttcca ctatcggttg cagggtaaac gcgggcaatt gctgctggga taattcttgc
     9421 ggaatagcaa tgttgacctg caaccgcgac tggaagcgcg ccttttcaat ttgcagataa
     9481 gcgttcacat gttcaatttc gtcggcgaga gtaacaaact ccgaaggccg ctttaagttt
     9541 ttgcggaaaa aagtagaaag atactgcacc agctggctgg cctgttcgct gtcgcggcgg
     9601 atcaccgctt taatggtgtt aagcgcatta aacaaaaaat ggggattcac ctgggcgtga
     9661 agcagtttga tctctgactg ggtgagcatc gctttttgcc gctcatattg cccggcgagg
     9721 atctgcgccg aaagcagttg cgcaatcccc tcgcccagcg tgcggttgat tgaactgaat
     9781 aaacggtttt tggcttcata caatttgatg gtacccatca ctcgctgatt ttcaccacgc
     9841 aacggaatga ccagcgtcga ccccagtttg cattgcggat gcaaagagca acggtaaggc
     9901 acttcgttgc catcagcgta gaccacttca ccggtttcaa tcgcttttaa ggtataagtc
     9961 gaagaaattg gtttgccggg taaatggtgg tcgtcaccaa ttccggtaaa ggccagcaat
    10021 ttctctcgat cggtaatcgc gactgcacca atatccagct cctgatacag cacctgtgcc
    10081 actttcatgc tgttcacttc gttaaacccc tggcgcaaaa tgccttccgt cgaggccgcc
    10141 actttcagcg cagtggcaga aaaagccgaa gtgtattttt caaacatcgc gcgtttatcg
    10201 agcaatatac gcataaacag tgccgcgccg acggtattgg tgaccatcat tggcgcagca
    10261 atattactca ccagacgcac cgcatcttca taaggtcggg cgatcgccag gatgatcaac
    10321 atttgcacca tttcagcgac gaacgtgacg gcaccggcgg taatggggtt aaagacttta
    10381 tcagtgcgcc cgcgacggat caggatgctg tgtaccaggc cgccgagcaa tccttcaacg
    10441 atggtcgaga tcatgcaact tagcgcggtc atgcccccca tcgaatatcg atgtaacccg
    10501 ccggtcagac caaccagccc accgacgacc ggaccgccga gtaagccgcc cattaccgca
    10561 cctatcgcac gggtattggc aatagaatcg tcaatgtgca acccaaacca ggtgcccatg
    10621 atgcagaaga tggaaaagac gatgtagcag agaaatttat gcggcagacg aaccgtgacc
    10681 tgcattaacg gtatgaataa tggcgtttta ctcattaacc acgcaatgac taaaaaaacg
    10741 cacatctgct gaagcagcag caacaccaga ttaaaatcgt acatacccgc aaaccacact
    10801 tccctttaaa acgcgtaaca tacattgctt gcgtttaact ttctttgaac tcttgcagaa
    10861 aaatgagaat tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa atcaccctat
    10921 agatgcacaa aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa agttaaaccg
    10981 ggacctcgcg agcaagggtg agacgatggc gctttacaca attggtgaag tggcgttgct
    11041 ttgtgatatt aaccctgtca cgttacgcgc gtggcagagg cgttacggat tgctgaaacc
    11101 acaacggaca gacggcggtc atcggctgtt caacgatgcc gatattgacc ggatccgcga
    11161 gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa gtaaaaatgc tgctcagtaa
    11221 tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa gaaacattac tgacttacct
    11281 gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa gagcgcggtc aggattaccc
    11341 cgcccagaca ctcaccacac atctgtttat tcctctgcgc cgacggcttc agtgccaaca
    11401 agcgactctc caggcgctgc tggcgatcct cgacggcgta ctgatcaact acatctctat
    11461 ttgcctggct tcggcgcgca aaaaacaggg taaagatgca ctggtggttg gctggaatat
    11521 tcaggatacc actcgtctgt ggctggaggg ctggattgcc agtcaacaag gatggcgcat
    11581 tgatgtcctc gcccactcgc tcaatcaact acgccctgaa ctgttcgaag gccgtacatt
    11641 gctggtatgg tgcggtgata atcgaacctc cgcccaacaa cagcaactca ccagttggca
    11701 ggaacaaggc catgatattt tcccactcgg catttaatga ttcgttaaca aatgcgcttt
    11761 actgtacaat cctttcgtta acataaggag tgcattatgc gcatagctaa aattggggtc
    11821 atcgccctgt tcctgtttat ggcgttaggc ggaattggtg gcgtcatgct cgcaggttat
    11881 acctttattt tgcgtgctgg ctgaacgcct gcaccagcct ttcaaacagg cggtctgcga
    11941 tgatcgccgc cagtgccacc agtaacgccc cctggatcac atacgcggta ttaaatccgc
    12001 taagcccgat gatgatcggc gtacccagcg tgctggcccc taccgttgag gcgatcgtcg
    12061 ccgtaccaat gttgataatc accgaagttc gcacgcccgc cagaatcacc ggagccgcca
    12121 gcggtagctc gaccttacgc agtcgctgac cacgactcat tcccatacct ttcgcaactt
    12181 ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt cgcctgcagg acgggcagca
    12241 cgccgtaaag gatcaaggcg ataatcgctg gtttcagacc aaagccgatc accggaacgg
    12301 caatcgccag cactgcaacg ggcggaaaag tctgtccaac ggcggcaata gtttccacca
    12361 gtggacgaaa tcccgcgccc caagggcgag tgacagcaat tccggcacca gtaccaatga
    12421 tcaccgcaaa caaactcgaa attcccacca gccagaaatg agccagtgcc agagctgcaa
    12481 agctttcctg ctgataaacg ggtcgtggca gttgtgggaa caaggcagca aacagcggct
    12541 ggctgtaagg cagccagaaa atcagcgcca caaacagagc aatcagccag aacagctgat
    12601 cgcgcaacat cttcatacga ttacgcctcc tccagcagat cctgaaaatg cagcgtgccg
    12661 caaggctcgc cctgcgtgtt caccaccggc agcacttcac atccccgcgc gacaaacagg
    12721 gagagcgcat cgcgtagcgt catctcttct gccagcgcct caccttctgc ccgttcttcg
    12781 cgacgcacgt aatccgccac gctacgtaac gaaagcaggc ggacacccag ttcactatgc
    12841 ccaaaaaact ggcggacaaa atcattcgcc ggatgagtca gcatcgccag aggattgccc
    12901 tgctgcacga cttcaccgtg atccatcaat accagatgtt ctgccagccg tagcgcctca
    12961 tcaatatcat gagtgaccag cacaatagtg cgcccctgca aacggtgaat gcgcgtcatc
    13021 tcttgttgca acgcgccacg cgttaccggg tccagtgcgc cgaatggctc atccatcagc
    13081 aagacttgcg gatcggcagc cagcgcgcgg gccactccca cacgttgctg ctgaccgccg
    13141 gaaagctgat gcggataacg ctcacgcaaa tccggctcca gccccagcag cgccattaat
    13201 tcgtcgatac gatcgtcgat ccgcgcccgt gaccattttt gtaattgcgg cacgctggca
    13261 atgttttgcg ccacgctcca gtggggaaac aggccaatag attgaatggc atagcccatc
    13321 cggcggcgca actccagcac tggcagcgag cgaatttctt ctccggcaaa gcggatcacg
    13381 ccactgtcat gctccaccag gcggttaatc attttcagag tggtggattt accggagcct
    13441 gatgtaccaa tcagcactga aaaactcccc tcctgaaaat tgagattgag atcgttaacg
    13501 gctttttgtg tgccgaacag tttgctgaca tggctaaatt caatcattac gtttcacctt
    13561 cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc accgccagaa caattaccgg
    13621 gatcaccccc agcaacacta aatcaatggc actgcttaac agcccctgaa aaaccagcgc
    13681 accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc atgccaacgg tttgcaccat
    13741 caccacccgc aggctgcgca gaaataccgg taacgccagc ggtaactgaa catgcaggaa
    13801 acgctgcgtc ccgctcatcc ccatcgctct ggcgctctcc agcacatcgc gcgggatctg
    13861 gttcaagccg actactacgc cgcgcaccag cggtaacagg gcatagagca ccagtgcaat
    13921 cagagcgggt gtcattccgg ttcctgctat gccgagcgtt cccagccacg gaaaggccgt
    13981 aaccagcgcg gcaagcggcg caatcaacag gccaaagagc gctaccgaag gcacggtctg
    14041 aatgacattc agcagagaaa aaatcgcccc ttgccgcgcg gtggaaaagt agcaccagat
    14101 gcccaacggc acaccaatca ctaacgcagg cagcaccgca ccaaacagca acgtcagatg
    14161 ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg tattctttca ttaacgagag
    14221 atcgttaagc gtgccggagt acagcaacca cagcggaaca atggcaatct gcatatgcaa
    14281 caaccagcgc cacagcggat gcgtggagat tcggcggatg gcatcgctac aggccagcag
    14341 tgccagcgcc gcagccagcc agaaaccact gccgaggctg gtacgcgcca gcgcactgcc
    14401 attttgcgcc agttgtgtcg ccgcctttcc agctccccac accagcaata cgaagacgaa
    14461 ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg ggaataaaac aggctgtcag
    14521 ccaggcgcaa ccaacgccca ccagcatcca gagagtttgc ggccacagct gccagagatg
    14581 acgcccctca cccgaaacta aacgattagg tgcgtaactg ataaacggca gcgccgctgc
    14641 gattgccgtc agcaacagca gcagcgccag aacaggatta atacggaaat aagtcacggg
    14701 aaattacttc gtccaccctt tttgtttcag gtagtcggct gccacttttt tggcatccag
    14761 tccttccact gcaatgctgg cattcagctg ctgcaatgtt ttttcatcga ggctggcgaa
    14821 gactggctgt agccactgtg ctatttgcgg atactctttc agcaccgact cacgcaccac
    14881 tggcgcaggc gcgtagatag gttgcacgcc ttgcggatcg cttaaggttt gcagccccag
    14941 cgccgcaacc gggccgtcgg tgccgtaagc cattgcggca ttaacaccag aagtttgctg
    15001 ggcagcggct ttgatcgtca ccgccgtgtc accaccggcc agtgacagca actgatcctg
    15061 accgagctta aagccatagg ctttttcaaa cgcgggtaag gcatcggcgc gttcgataaa
    15121 ctctgccgag gctgccagtt tgaagttgcc accctctttc agataacgac tcagctcggc
    15181 aagcgaagtg agtttgtttt tctctgccac atcctgacgc acggcgatgg tccaggtgtt
    15241 atttgcaggc gcgggcgtca gccagattaa cttgttttgc tctgcatcga gttttttgac
    15301 tttctcatag ccttgctgcg cgtttttcca tgccgtatcg ttttcatctt taaagaaaaa
    15361 cgcgccattg ccagtatatt ccggatagat atccagctcg ccagaagtga tcgccccgcg
    15421 cactacggga gtcgttccca gctgcacttt atttaccgta ggaacgccgt gactttccag
    15481 cacctgcaaa atgatattgc cgagcagcgc gccttccgta tcgatttttg aaccaacctt
    15541 aacgggggaa gccgcctgta gcggcaggct cacggctgcc aacaaaacca gtgaacctgc
    15601 ccagaccttt gagagtggca tgatgctttc ctcattcttt tactgttgtt ttcagcgaat
    15661 taagagaaaa gcatagttga taatggcgga gttagcctga ctgggtggac tcagttgcag
    15721 aatcagataa atacttaaaa ggcatatttt gcccggcgtt tcttgccgga tgcggcgtaa
    15781 acgccttatc cggcatacaa acatcgaaaa ttacaacaac tcaaactcgc ctttcttaac
    15841 gcgtgcggaa tcagtgccga tgaagacatt gaacttgcca ggctcggcgt catatttcat
    15901 ctgttgattc cagaacttca gcgcctcaat atcgattggg aagctgacgg tctgagtttc
    15961 gcccggtttc agggtgattt tctcaaagcc tttaagctgc ttcaccgggc gactcatgga
    16021 agccgtcaca tcctgcaagt acatttgtac taccgtggcc ccttcgcgtt taccggtgtt
    16081 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
    16141 cacatcagag acggtgaaag tggtgtagct cagaccatag ccgaacggat aaagcgcccc
    16201 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
    16261 cccggtattc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
    16321 cggcagcttg ccggacgggt tataatcgcc aaacaataca tcggcaattg cattaccgcc
    16381 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
    16441 cgccagcgga cgcccattca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
    16501 cgcaatcaag tcacgttggc tttgcggaat agtgatatcg gtacgactgg aagcttcgtg
    16561 cgccatcccc tgagcttcac cgaccacagc taccaccaca tcagattgtt tcgcggtttg
    16621 caccgcttca tcaatcatct cttgcggcga acgcggatca actttgaccg cctcttcata
    16681 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
    16741 cactttaccg ttctcaccga cggcgttttt aatccctgtt agcacagtaa cagattgatc
    16801 ggcgacacct gctgccgacc agcttcccat cacgtcacgc ttgctgtctg ccagcgggcc
    16861 aaccaccgca atggttgcag attttttcag cggcagcgtt tcgagacggt ttttcagcaa
    16921 caccaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
    16981 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaatcc
    17041 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctt ccatcgtcac
    17101 tttgccggat ttgatcaacc caggcagata cttcgagtaa tactcgtcgc tcatgctcat
    17161 gttgattccg gacttcagcg ccacgcgtac cgcatcttcc gggtctgccg ccgtgccatg
    17221 tttaatcagt tctttgattg cgccgtgatc ggaaacggtg atgcctttaa agccccactg
    17281 gtcgcgcaga acatctttca gcagccagga gtcggaggtg gctggcgtgc cgttcagcga
    17341 gttcagcgcc accatcaccg caccgctgcc tgcatccagc cccgctttgt acggcagcat
    17401 atagtcgtta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
    17461 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
    17521 gcttttaccc tgcatcgctt ccaccatggt tttgcccatt attgaggtga gatacgtatc
    17581 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
    17641 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgactgt
    17701 tttcaccgca tcgaggttaa aagacgaggc cagaccgaga ctaatcggga acaccgtgcg
    17761 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
    17821 cacctgatcc tgcatggcgc ggatatcctg acgggttaca gtgttgaaaa tcgccccaac
    17881 ctgaccgtct ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat
    17941 taagcgcagc tgaccaattt tctcgtcaac tgtcattttc ttaagcagtt cggtgacgaa
    18001 cgcatcccgc gcttcgggcg ttaatggatg gttgccgaat aaatcatccg ccagtgctgg
    18061 ctgcagggcc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
    18121 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
    18181 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
    18241 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
    18301 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
    18361 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
    18421 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
    18481 cgggtgctga aagcctgcgt cagcgctgac aaaattattc tgatgcaggc tgccaataca
    18541 ggcctgaccg aaggatcgac gccaaacggt aacgattatg atcgcgatat cgtgatcatc
    18601 agcaccctgc gtctcgacaa gctgcacgta ctcggtaagg gcgaacaggt gctggcctat
    18661 ccgggcacca cgctctattc actggaaaaa gccctcaaac cgctgggacg cgaaccgcac
    18721 tcagtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
    18781 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
    18841 aatgaagacg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
    18901 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgccacgat
    18961 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccaacacg
    19021 cccgcacgtt ataacgctga tccggatcgg ttatttgaat cttctggttg cgccgggaag
    19081 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
    19141 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaat
    19201 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
    19261 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
    19321 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
    19381 cattttaccg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
    19441 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat
    19501 ggcgtcggtg aagccaaatc gtggctagtg gattatttca aacaggccga gggcgatttc
    19561 tttgtctgta cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc
    19621 gcagcaattc gttatcaggc tgtgcattcc gatgaagtcg aagacattct ggcgctggat
    19681 atcgctctgc ggcgtaacga caccgaatgg tatgagcatt taccgccgga gatcgacagc
    19741 cagctggtgc acaagctcta ttatggccat tttatgtgct atgtcttcca tcaggattac
    19801 atcgtgaaaa aaggcgtgga tgtgcatgtg ttgaaagaac agatgctgga actgctacag
    19861 cagcgcggcg cgcaataccc tgccgagcat aacgtcggtc atttgtataa agcaccggaa
    19921 acgttgcaga agttttatcg cgagaacgat ccgaccaaca gtatgaatcc ggggattggt
    19981 aaaaccagta agcggaaaaa ctggcaggaa gtgaaataaa aattacggat ggcagagtat
    20041 cgccatccgt acttcattta atcgttctgt gccgtctgcc ccgccgccgc catttgggcg
    20101 gctttttgtt ttttatagct caacgctgct gccggaacag gcatcacttt accggtttca
    20161 atccaggtac gcaggcggct ggcatcggca aaatgggtat atttgccaaa cgcgtccatc
    20221 actaccagcg ccaccggttt attattgata accgtgcgca tcgccagaca atggcccgcc
    20281 gcattggtaa aaccggtttt ggttaactga atattccagt tatcacgata caccagatgg
    20341 ttagtattgc ggaacggcag cgtatacgtc gggttagaga aggttgccat atcttcccgg
    20401 gtagtactta actgcccgat caacggatat tgtttgctgg caatgagtag tttggttaag
    20461 tcacgggcgg ttgaaacgtt atgcaccgac aatccagttg gttcaacaaa gcgcgtgttg
    20521 ttcattccga gcgatttcgc tttcgcattc atcgccttaa taaaggcttt gtaaccaccg
    20581 ggataatggt gcgcaaggct tgccgccgcg cggttttctg aagacatcag cgccagcaac
    20641 agcatatctt tacggctgat ttcgctgttc agtcgtacgc gcgaatagac ccctttcatc
    20701 tccggcgtct ggctgatatc cacttttagt ttttcatcca gcggcagtcg tgcatccagc
    20761 acaaccatcg cggtcattaa tttgctgata gacgcaatcg gacgcaccag atccgggtgg
    20821 ttcgaataga tcactttgtt ggtattcaga tcaacaatca tcgcgctacc ggaggcaatt
    20881 tccggttgtg acgcggtggt agcggctgcc gttttcgcaa cggcctgcgg tgcaaaaggc
    20941 acagccagca tcagggccag gctaaataaa gaaactcgaa atttcggcat gatgagcatt
    21001 cagatagtgg ttcacgcgca cgggttgcgc accgccggag taaggattta ctgaggctag
    21061 cgacgccatc ataacgagca aaaagtgcga tcgtcaaaga agaatcgtga ggaaatgctg
    21121 cattgctgac atttacgcca gcaatgcagc gcaaaagaac tttctagaac aaacgataac
    21181 cgtagcccca tagtataacg gtaagggcga gcagcacttc cagcaccagc acgcctatcg
    21241 ccagtgtcga actggagaag ctaagtcctt cctctttgtt gatattcagg aagctcggaa
    21301 taccgagata cagcaggtag ccggtgtaaa acagcgccac cgtgccgacc agcgcacaca
    21361 accagaccag cgggtaaagc gccaccagac cgcttaaaaa cagcggagtt gcaacgtagc
    21421 ccgcgaagac catgcagtgc gcaagtgacg gacgctgcgg ataattacgc gccatccacc
    21481 agatgacccg ccccatcacc gcaaccccag ccagcataac gccgtaaaac aagacagcca
    21541 gcgccagtcc ggtaaaccag gataacttca ggatagtgcc atcgccaaaa ttccagccaa
    21601 tctgtgtagt gccaatgaag gcgcaaatca ccggaatcgc cgccatcagc aaaacgtggt
    21661 gggtgtagtg atgagaaatc gtttcgtttt cgcgattaat cacctgcatt tcacgatcgg
    21721 gatgggaaaa cagtccccag acatggctca taccgccccc ttgttgtgag ttcatgaacc
    21781 tgacagttca agtataagtc aggttgtgat tattttttgt tcgctcatgt aatttgcacc
    21841 gtctgatgta cgtccttttt cagcgtgaat gattcacagg gtgtatgctt acagtcaagc
    21901 aaagggagac agactgtcct atggatctca atacactcat ctcacaatat ggttatgccg
    21961 cgctggtgat cggtagcctg gcggaaggtg aaaccgtgac tttgctggga ggcgttgcgg
    22021 cacatcaggg gctattaaag ttcccgctgg tggtactttc tgtggcgctt ggcggcatga
    22081 ttggcgacca ggtgctttat ctgtgcgggc ggcggtttgg cggcaagctg ttacgccgtt
    22141 tctcgaaaca tcaggataaa attgagcggg cgcagaaact tatccaacgc catccgtatc
    22201 tgtttgtcat tggtacgcgt tttatgtatg gctttcgggt cattggcccg acgctgattg
    22261 gtgccagcca gctgccgccg aaaatctttc tgccgctgaa tattctcggc gcatttgcct
    22321 ggtcgctgat ttttaccact atcggttacg ctggtgggca ggtgattgcg ccgtggttgc
    22381 acaatctcga ccagcatttg aagcactggg tctggttgat tctggtcgtg gttctggtgg
    22441 taggcgtgcg ctggtggctg aaacggcgcg ggaagaaaaa gccggatcat caggcgtaaa
    22501 actattgccc tggataaggt gttcgcgccg catccgacat ccggtgcacc atgcctgatg
    22561 cgacgctggc gtgtcttttc tggcctaacc caatgaaata accacgcacc tcactgcgct
    22621 attctgggtt gaactgcgga ttcgccaaca taaagccgcc atccactatc aacgactgcc
    22681 cggtggtgta atttgcgccc tccgaacaaa gccataccac caggctggca atctcatgcg
    22741 ttgtgccaaa acgtcgcaag ggaatcgaag gctccgcgtc gggcttcacg tcgccgccat
    22801 ccatgccgtt cattggcgtg gcgatcgccc caggtgcgac tgcgttcacc aaaattttat
    22861 gcctgaccaa ctccagcgcc attgctttgg ttaacccgcc gagcgcatgt ttagcggctg
    22921 tgtaggcgct ggcatccggc agcggcgtat gttcatgtac cgatgtaatg ttgatgatgc
    22981 gaccgccctg cccttgcttc accatctggc gggcagcaat ttgcgagcat aaaaatgcac
    23041 catcgacatc aacggtaaaa atcttgcgcc actcatcgaa agccatatca agaaacggcg
    23101 ctttggtcat tgcgcccgca ttattcacca gcacatcaat acgccccagc cgttgaataa
    23161 gtttctccag cgcctgcgca ccttctggta gattgccgag atccagttgc acgatctccg
    23221 cacgtacgcc gtggctaact acctcacgcg cggtatcttt tgccccttct tcatctgagt
    23281 gccaggtaat gccaatatca aacccctgct gcgccagcaa taacgcgcac tctttgccga
    23341 tccccgaatc ggaggcggta attatcgcga cctgtgccat cgagttctcc acttaacgct
    23401 caacaaacgt taagtataga aggcgcatat catcagcgtt tgtacccccc gcccagcgca
    23461 ccagtgagtt gaatggaggc atccagccac tgcccttgca ataacaggcc attggcccgc
    23521 tcacgcagcg ccgggattct ggcttcgctg acccgggaac cagcaatgag gcccgcgtta
    23581 aagcgggcct gcgcaagacc tactacacgc aaggcatcgc gctcaatttg cgcctgatgc
    23641 tggtttttct ccgccagtgt ctgaacctga ctggctgccc gcgccacgtc attcaccgct
    23701 tcaaccaccg ctttgttgta gctggcgata gacaagttgc tttcggcttt tgcgatatcg
    23761 aggttggcgt taagacgacc actatcgaaa atgggtagcg tcaggcctgc cgtaacgccc
    23821 atttgctgcg cggaatgacg gaacagatcg cttaagtgca acgcatcctg ttgcaggaag
    23881 gccatcaggt tgatgtcagg ataaaatgcc gcttttgccg catcaatagt gcttaacgat
    23941 gactcaacgt accagtgcgc cgcctgcaaa tctgcccgcc gcgccagtaa ggagtacccc
    24001 agttcatcag gaagctggct tgccactttc ggcaacgcga ccggtttaag cttcagtgac
    24061 tttgtctggt gatttgtgag tgcatttaac cgtgcctcaa taattttcat tttacccgcg
    24121 acatcgttga gctgctgccg ggttttgctg gcattaatat cggtttccac accttcaact
    24181 gaagaagtaa tcccgttctg atatagctgg cgatcggtcg cgataatggt gttctgctct
    24241 ttttcgattt gctgcaagac cgtgtttaac gccgcctggg tttgccactc ccagtacagg
    24301 cgggctacgc tgccagccag caattggcgg gtttgctcgc gttccgccgc ccgtgcttta
    24361 accgtaccca gtcgagcagt aacctccgcc cgattctttc cccagatatc gagatgccag
    24421 cccgccgtta agccaaaagt accgttggtg taccacggac cggtcgtacc tgcggccgga
    24481 tcgttcaaag caaacggccc cattaaccct tctgccgaca ttttttgccg ctccatatcc
    24541 gcagaaaagt cgagctgcgg accatcctga gtggcaactg ccttcgcctg ggcttcagcc
    24601 agctgaatgc gctgttcagc cacctgcata tccggtgcgt tctgtagtgc attgttaatt
    24661 aatgaagtga gttgatcatc gtgatactcc agccaccatt ggctgtctgg ccaaccattt
    24721 ttcagtgccg tgggtaatgc ggtgtcaact tgcacagcgg gcgtgtgctg gcttaacgcc
    24781 tggcgggttt catgcatagg cgcacacccg gccagcatca gtaacagcgg aaaacaggcg
    24841 atggcgggat aaaaggaatc acgattcatg ggggaataat caggtaagaa aaggtgcgcg
    24901 gagattaccg tgtgttgcga tatatttttt agtttcgcgt ggcaatacat cagtggcaat
    24961 aaaacgacat atccagaaaa atatacacta agcgaatgat atctcccgat ttatcttaat
    25021 cgtttatgaa tagcggcaaa gggcttcgtt ttttcctata cttattcagc actcacaaat
    25081 aaaggaacgc caatgaaaat tatactctgg gctgtattga ttattttcct gattgggcta
    25141 ctggtggtga ctggcgtatt taagatgata ttttaaaaat cagatatggc atataagttg
    25201 tcaggtcagg aactagatat tttgtgtact tgatttgcaa acagctccgg tgttggtggc
    25261 gtcaaaatcg ctgaagcgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg
    25321 gcgcgtctta caaggacgtt acctcgcgcc cgacccggta gccgaagggg ataagctgaa
    25381 ggtaccgcgc agcggcgatt ttgttcgcca gagcccgggg gtgcaggggg aggaggcgat
    25441 tgtcctcccc ctgcgcgctc cttgcgccag tggcaatatg tggcttagct catgaaagga
    25501 gcgcaacaag atgatgaagc tgaataagcg attcattgtc tcagataagc tgcgattcca
    25561 ctattatcgc agcttatcga tatcaatggc ctgaatagcc cttgcaatat caggggaatt
    25621 attcaacacc cgaacatgct gaaataattc cgttgcttca tcgtattctt tacgcaaata
    25681 actcaaccac tgtttaatcc gcgcaacgtg atataacccg gtatcgccct gcttttccag
    25741 acgggtatat ttttgcagca aagcaaccac ctccggccac ggcattcgcg gttcgttata
    25801 tttcaccaca cggctcaggt tgggaatatt gagcgccccg cgaccaatca tcactgcgtc
    25861 gcagccgctg atcgccatgc attgttgcgc actctgccag tcccagattt caccgttggc
    25921 aatcaccgga atattaagcc gctggcgaat ctcgccaatc gcctgccagt caatatgttc
    25981 cgcgcggtaa ccctgctctt ttgtccgccc atgtactacc agctccgtag caccagcctg
    26041 ttgaaccgca tcggcgattt caaatttctt ctcaccgctg tcccagccca gacgcacttt
    26101 tacgctgaca ggcaaatgcg ccggtacagc ttcgcgcatc gcttttgcac cctggtagat
    26161 gagttcagga tctttcagta acgttgcccc accaccgcta ccgttaaccg ttttcgacgg
    26221 gcagccgcag ttaagatcca cgccccagga acctaactcc accgcacggg cggcgttctc
    26281 tgccagccac tgtgggaact gccctaacaa ctgcacgcgc accagcgtac cggatggtgt
    26341 ccggctggca ttttgtagct cagggcaaat gcgatgaaag acttttactg gcagcagttg
    26401 atccaccacg cggacaaact cggtgatgca cagatcgtag tcgttaactt cggtcagtaa
    26461 ttcccgcacc agagagtcaa gtacaccctc catcggagcc agtaacacac gcatatcatc
    26521 acccgcaaaa aaatgaggcg ctatgttagc gcctccggtc aacggattaa aggtctgcaa
    26581 agagggattg ctgcaaactg caatccctct ctatgcttga tgtatgcctg agttcgctgc
    26641 gcattgcgta ataccgggga gcgaaaaaat aagcgctgat ttcttaatgt gatcggtagc
    26701 acgttttacg aattaattgt atgatgaatc catctcatct ggggtgttga ttatgagtaa
    26761 gacactgaac attatctggc aatatttacg cgctttcgtc ctgatttatg cctgcctgta
    26821 tgcaggcatt ttcattgctt ccctgctacc ggtaaccatt ccgggcagca tcatcgggat
    26881 gctgatcctg tttgtcctgc tggccttgca aattcttccg gcgaaatggg tcaatccggg
    26941 gtgctacgta ctgattcgct atatggcgct attgtttgtg ccgattggcg taggcgtcat
    27001 gcaatatttt gatttgctcc gcgcacagtt tggtccggta gtggtttcct gtgcagtcag
    27061 tacgctggtg gtttttctgg tggtgagctg gagttcacaa ctggtgcacg gtgaacgtaa
    27121 agtcgtaggt cagaaaggat cagaagaatg atggcgaata tctggtggtc attaccgctg
    27181 acgttgattg tcttttttgc cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt
    27241 aacccgttgc tggtagcaat ggtggtgatc attccttttt taatgctgac tggcatctct
    27301 tacgacagct actttaaagg tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc
    27361 gcactggctt atcctttata tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc
    27421 atcaccatct gttttatcgg cagcgtggtt gcgatggtga cggggacttc cgtggcattg
    27481 ttgatgggcg cttcaccgga aatcgccgcg tcaatcctgc cgaaatcagt taccacgcct
    27541 attgcaatgg cggttggcgg cagtattggc ggtattccgg caatcagcgc agtttgcgtg
    27601 attttcgtcg gcatcctcgg cgctgtgttt ggccatacat tgcttaatgc gatgcgtatt
    27661 cgtaccaaag ctgcgcgcgg tctggcaatg ggcacagcct cgcacgccct cggtacggcg
    27721 cgctgcgccg agctggatta tcaggaaggt gcatttagtt cgctggcgct ggtgttatgc
    27781 gggattatta cttcgctgat cgcaccgttc cttttcccga ttattctggc agtaatgggc
    27841 taaaatttgc gatgcgtcgc gcatttttga tgtaagtttc acgcgttgca taattaatga
    27901 gattcagatc acatataaag ccataacggg ttcgtaaact gttatcccat tacatgatta
    27961 tgaggcaacg ccatgcatcc acgttttcaa accgcttttg cccaacttgc ggataacttg
    28021 caatctgcac tggaacctat tctggcagac aagtacttcc ccgctttgtt gaccggggag
    28081 caagtctcat cgctgaagag cgcaacgggg ctggacgaag acgcgctggc attcgcacta
    28141 cttccgctgg cggcagcctg tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt
    28201 gcgcgcggtg tgagcggaac ctggtatttc ggtgccaata tggaatttat tggtgcgaca
    28261 atgcagcaaa ccgttcatgc cgaacaaagc gcgatcagcc acgcctggct gagtggtgaa
    28321 aaagcgcttg cagccatcac cgttaactac acgccttgtg gtcactgccg tcagtttatg
    28381 aatgaactga acagcggtct ggatctgcgt attcatcttc cgggacgcga ggcacacgcg
    28441 ctgcgtgact atctaccgga tgcctttggg ccgaaagatc tggagattaa aacgctgctg
    28501 atggacgagc aggatcacgg ctatgcgctg actggtgatg cgctttctca ggcggcgatt
    28561 gcggcggcaa accgttcgca catgccttac agtaagtcgc caagcggtgt cgcgctggaa
    28621 tgtaaagacg gtcgtatttt cagcggcagc tacgctgaaa acgccgcatt caacccgact
    28681 ctgccgccgt tgcagggggc gttaattctg ttgaatctca agggttatga ttacccggat
    28741 atccagcgcg cggttctggc agaaaaagcc gatgcgccgt tgattcagtg ggatgccacc
    28801 tccgcaacgc tgaaagctct cggctgtcac aatatcgacc gcgtgcttct cgcttaagcc
    28861 tggagccgga tgcggcgtga acgccttgtc cggcttgcca gccctttcct ggtgtcgaaa
    28921 atcccggcaa acagtttgcc gtttcttgcg caaaaccagc gggtaaagta gcctgatgga
    28981 aattttcctt agatcgagtc tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc
    29041 tgatcggctt gctgctgttg actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg
    29101 cgccttatat ctacgacgaa ttgcaggatc tcccctaccg ccaggtcggc gtggtgctcg
    29161 gcacagcaaa atattatcgt actggcgtaa ttaatcagta ttatcgctac cgcattcaag
    29221 gagcgattaa tgcctataac agcggtaagg taaattatct attactgagc ggcgataacg
    29281 cattgcaaag ttataatgag ccgatgacca tgcgcaaaga tttaatcgca gctggtgtcg
    29341 acccatcaga tattgttctc gattacgcag gctttcgtac gctggattcc atcgtgcgta
    29401 cacgcaaagt tttcgatact aatgatttca ttattatcac ccaacgtttc cactgtgagc
    29461 gggcattatt tattgcgctg catatgggga ttcaggctca gtgttatgcc gtaccgtcac
    29521 cgaaagatat gctgtcagta cgtattcgcg aatttgccgc ccgtttcggt gcgctagctg
    29581 acctttatat ttttaaacgt gaaccgcgtt ttttagggcc gctggtccct attccggcta
    29641 tgcaccaggt gccggaagat gcgcaggggt atcccgccgt cacacccgaa cagttacttg
    29701 aattacaaaa gaaacaagga aagtagttat ggatgtacag cagttttttg tcgttgccgt
    29761 ttttttcctt atcccgatat tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc
    29821 gattgataaa cgggttaaaa atgcaccaga accggtgtat gtctggcgag cgaaaaatcc
    29881 cggactcttc ttcgcttata tggtggcata tatcggcttc ggaattttat ctatcggcat
    29941 gattgtttat cttattttct atcgttaata tt
//
LOCUS       DADTFG010000048        29962 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.048, whole genome shotgun sequence.
ACCESSION   DADTFG010000048 DADTFG010000000
VERSION     DADTFG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.048"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(136..1497)
                     /gene="rhlE"
                     /locus_tag="J1138_001968"
     CDS             complement(136..1497)
                     /gene="rhlE"
                     /locus_tag="J1138_001968"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBA8453771.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQSRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            1726..2397
                     /gene="cecR"
                     /locus_tag="J1138_001969"
     CDS             1726..2397
                     /gene="cecR"
                     /locus_tag="J1138_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBA8453772.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRTAIRELILRV
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2397..3395
                     /gene="hlyD"
                     /locus_tag="J1138_001970"
     CDS             2397..3395
                     /gene="hlyD"
                     /locus_tag="J1138_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBA8453773.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLVAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPNKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3388..5124
                     /locus_tag="J1138_001971"
     CDS             3388..5124
                     /locus_tag="J1138_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8453774.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDAALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5117..6250
                     /locus_tag="J1138_001972"
     CDS             5117..6250
                     /locus_tag="J1138_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8453775.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEQLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6261..7367
                     /locus_tag="J1138_001973"
     CDS             6261..7367
                     /locus_tag="J1138_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8453776.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7329..7739)
                     /locus_tag="J1138_001974"
     CDS             complement(7329..7739)
                     /locus_tag="J1138_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453777.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFVL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7872..8633
                     /locus_tag="J1138_001975"
     CDS             7872..8633
                     /locus_tag="J1138_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBA8453778.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTSFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRTHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8630..9871
                     /gene="clsB"
                     /locus_tag="J1138_001976"
     CDS             8630..9871
                     /gene="clsB"
                     /locus_tag="J1138_001976"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBA8453779.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVGP
                     PAQPTMETQDRVETENTGVKP"
     gene            9871..10827
                     /locus_tag="J1138_001977"
     CDS             9871..10827
                     /locus_tag="J1138_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBA8453780.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALTQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10863..11576)
                     /locus_tag="J1138_001978"
     CDS             complement(10863..11576)
                     /locus_tag="J1138_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBA8453781.1"
                     /translation="MESYSQNSNKLDFQHEARILNSIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSSVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11782..12486)
                     /locus_tag="J1138_001979"
     CDS             complement(11782..12486)
                     /locus_tag="J1138_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBA8453782.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12623..13075)
                     /gene="moaE"
                     /locus_tag="J1138_001980"
     CDS             complement(12623..13075)
                     /gene="moaE"
                     /locus_tag="J1138_001980"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBA8453783.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTCAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13077..13322)
                     /gene="moaD"
                     /locus_tag="J1138_001981"
     CDS             complement(13077..13322)
                     /gene="moaD"
                     /locus_tag="J1138_001981"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBA8453784.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(13315..13800)
                     /gene="moaC"
                     /locus_tag="J1138_001982"
     CDS             complement(13315..13800)
                     /gene="moaC"
                     /locus_tag="J1138_001982"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBA8453785.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13803..14315)
                     /gene="moaB"
                     /locus_tag="J1138_001983"
     CDS             complement(13803..14315)
                     /gene="moaB"
                     /locus_tag="J1138_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBA8453786.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14337..15326)
                     /gene="moaA"
                     /locus_tag="J1138_001984"
     CDS             complement(14337..15326)
                     /gene="moaA"
                     /locus_tag="J1138_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBA8453787.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGIELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15314..15458)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15723..16631
                     /gene="yvcK"
                     /locus_tag="J1138_001985"
     CDS             15723..16631
                     /gene="yvcK"
                     /locus_tag="J1138_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBA8453788.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16669..18690)
                     /gene="uvrB"
                     /locus_tag="J1138_001986"
     CDS             complement(16669..18690)
                     /gene="uvrB"
                     /locus_tag="J1138_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBA8453789.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19269..19946)
                     /gene="bioD"
                     /locus_tag="J1138_001987"
     CDS             complement(19269..19946)
                     /gene="bioD"
                     /locus_tag="J1138_001987"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBA8453790.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRISSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESSVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPLILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(19939..20694)
                     /gene="bioC"
                     /locus_tag="J1138_001988"
     CDS             complement(19939..20694)
                     /gene="bioC"
                     /locus_tag="J1138_001988"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBA8453791.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGLHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(20681..21835)
                     /gene="bioF"
                     /locus_tag="J1138_001989"
     CDS             complement(20681..21835)
                     /gene="bioF"
                     /locus_tag="J1138_001989"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBA8453792.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21832..22872)
                     /gene="bioB"
                     /locus_tag="J1138_001990"
     CDS             complement(21832..22872)
                     /gene="bioB"
                     /locus_tag="J1138_001990"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBA8453793.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTVVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            22959..24248
                     /gene="bioA"
                     /locus_tag="J1138_001991"
     CDS             22959..24248
                     /gene="bioA"
                     /locus_tag="J1138_001991"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBA8453794.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLSVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTATVNRAVQDETFFCQ"
     gene            complement(24359..25489)
                     /gene="tnpB"
                     /locus_tag="J1138_001992"
     CDS             complement(24359..25489)
                     /gene="tnpB"
                     /locus_tag="J1138_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HBA8453795.1"
                     /translation="MKRAYKYRFYPTTEQAELLAQTFGCVRFVYNSILRWRTDAYYER
                     KEKIGYLQANARLTALKKEPEYIWLNDVSCVPLQQSLRHQQAAFANFFAGRAAYPAFK
                     SKRHKQVAEFTASAFKHRDGELYIAKSKSPLDVRWSRELPSAPSTVTISRDSAGRYFV
                     SCLCEFEPVSMPVTAKTVGIDVGLKDLFVTDTGFKTDNPRHTAKYAKRLTLLQRRLSR
                     KQKGSRNRIKARLKVARLHAKIADCRMDNLHKLSRKLINENQVVCVESLKVKNMIRNP
                     KLSKAIADAGWSELVRQLQYKGKWAGRSVVAIDQYLPSSKCCSCCGFTMQKMPLNVRK
                     WHCPECGADHDRDINAARNIKAAGLAVLAHGEPVNPESQHAA"
     gene            25598..26074
                     /locus_tag="J1138_001993"
     CDS             25598..26074
                     /locus_tag="J1138_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBA8453796.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            26226..27509
                     /locus_tag="J1138_001994"
     CDS             26226..27509
                     /locus_tag="J1138_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBA8453797.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(27649..29910)
                     /locus_tag="J1138_001995"
     CDS             complement(27649..29910)
                     /locus_tag="J1138_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBA8453798.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMADVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
BASE COUNT         6957 a         8040 c         7830 g         7135 t
ORIGIN      
        1 gagcgcctta tccggcctac aaaaacatgc acattcaata tattgcagag ttcacgtagg
       61 cctgataagc gtagcgcatc aggcagtttt gtgtttgtca tcagtctcat gccgggcata
      121 gcccggcata aaagattact gcgcagcggc aggtttacgc ggacggcggc gacgttgttg
      181 ttcgcctgcc ggtttggcat cgccgagacg acgagacggt ttttctgcgg gcttcgcgtt
      241 accagacttc gcaccacttt ccgcacggcg tgattgctgt tgaccgcgac caccgccctg
      301 tccacgaccg ccgccacgtt gctggcgacc gttctggatc ggttcggctt tgattgacgg
      361 gtccggctca taccccggaa tcgcaatgcg cgggatctct tttttcagca gtttttcgat
      421 atcacgcagc agtttgtgtt catcaacaca caccagcgac aatgcttcgc cagttgccgc
      481 cgcacggcca gtacgcccga tacggtgaac ataatcttcc ggtacgtttg gtaactcata
      541 gttgacgacg tgcggcaact cttcgatatc cagaccgcgc gcagcgatgt cagttgccac
      601 cagtacacga atatcacccg atttaaaatc ggccagcgca cgagtacgcg caccttgcga
      661 tttattaccg tggatcgccg cactacggat gccgtcttta ttaagctgtt cagccagatg
      721 gttagcgcca tgtttggtac gggtaaacac cagcacctgc tgccagttcc ctttcccaat
      781 catatgcgac agcaattcgc gtttgcgttt cttatcgaca aagtgaacgt gctgggtcac
      841 ctgatcagac gcagtattgc ggcgtgccac ttcgatttcc agcgggttgt gcaacagttt
      901 ttccgccagg gctttaatat cgtcagagaa ggtcgcggag aacaacaggt tctggcgctt
      961 cgctggcagt tttgttaaca cgcgacggat atcgtggata aagcccatgt cgagcatgcg
     1021 gtcagcttca tcaaggacga ggatttcaac ctgatccagc ttcactgcat tttgatgttc
     1081 caggtccagc agacgtcccg gcgttgccac cagcacatca acgccgccgc gcaacttcat
     1141 catttgtggg ttaatgctga caccgccaaa caccaccagc gaacgaatgt tcagatattt
     1201 gctgtaatca cggacgtttt cgccaatctg cgccgccagt tcacgggtcg gggtaagaat
     1261 gagcgcacgt accggacgac gccctttggc gtgcggctgg cgagtgatca gatgttgcaa
     1321 cagcggcagc gtaaagcccg ctgttttgcc ggtaccggtc tgggcgctgg ccatcaggtc
     1381 gcggccttcc agcaccgcag ggatcgcctg ctgctgaata ggggtgggtt cacggtaacc
     1441 ctgctcggca acggcgcgca ggatatcagg gcttaaaccc aaagaatcga aagacataac
     1501 tactccgaac cgccccgacc gttaccggtg tagttttcag ggagatactg aaaaggaaaa
     1561 tgacaaaaac cacaatgtca tgagggagcg gagtgtagca gtttttgtga cgcagcgcat
     1621 aaattatccc tgtgactggc gtagctaaaa ttcttagcca tactggacaa ctcccctatg
     1681 gagtcataat cttaatcaat catttgatta agaatggact gtgcgatgaa taatcctgcc
     1741 atgacaatca agggtgaaca ggcgaaaaaa cagctgattg ctgcggcact ggcacagttt
     1801 ggtgaatatg gaatgaacgc caccacccgc gagatagccg cccaggccgg gcagaatatc
     1861 gctgccatca cctactactt cggttcgaaa gaagatttgt acctcgcctg cgcccagtgg
     1921 attgctgatt ttattggcga gcagttccgt ccgcacgccg aagaagccga acgtttgttc
     1981 gcacaaccac agccggatcg gacagccatc cgtgaactga tccttcgcgt ctgcagaaac
     2041 atgattaagc tgctcaccca ggatgacacc gtcaacctca gcaaatttat ctcccgtgag
     2101 cagctctctc ccacggcagc ctaccacctg gtgcacgaac aggtgattag tccgctacac
     2161 agtcacctta cacgcctgat tgccgcctgg accggctgcg acgctagcga tacccgcatg
     2221 atccttcata cccatgcgtt gattggtgag attctggcgt ttcgtcttgg caaagaaact
     2281 attctgttac gtaccggctg gaccgcgttc gatgaagaaa agaccgaact gatcaaccag
     2341 acggtgacct gtcatattga cctgattttg caaggattat cgcaaaggag tttgtagtga
     2401 tgaaaaaacc tgtcgtgatc ggactggcgg tagtagtact tgccgccgtg gttgccggag
     2461 gctactggtg gtatcaaagc cgccaggata acggcctgac gctgtatggc aacgtggata
     2521 ttcgtacggt gaatcttagt ttccgtgttg gggggcgcgt tgagtcgctg gcggtggacg
     2581 aaggtgatgc gatcaaagcg ggccaggtgc tgggcgaact ggatcacaag ccgtatgaga
     2641 ttgccctgat gcaggcgaaa gcgggcgttt cggtggctca ggcgcagtat gacctgatgc
     2701 ttgccgggta tcgcgacgaa gaaatcgctc aggccgccgc agcggtgaaa caggcgcaag
     2761 ctgcctatga ctatgcgcag aacttctata accgccagca aggactgtgg aaaagccgca
     2821 ccatttcggc aaatgacctg gaaaatgccc gctcctcgcg cgaccaggcg caggccacgc
     2881 tgaaatcagc gcaggataaa ttgcgtcagt accgttccgg taaccgcgaa caggacatcg
     2941 cccaggcgaa agccagcctc gaacaagcgc aggcgcaact ggcgcaggcg gagctgaatt
     3001 tgcaggattc gacgctggtc gctccgtctg atggcacgct gttaacgcgc gcggtagagc
     3061 caggcacggt gcttaatgaa ggtggcacgg tatttaccgt ctcgctgacg cgtccggtgt
     3121 gggtacgcgc ctatgttgat gaacgtaatc ttgaccaggc ccagccgggg cgcaaagtgc
     3181 tgctttatac cgatggtcgt ccgaacaaac cgtatcacgg gcagattggt ttcgtttcgc
     3241 cgactgctga atttaccccg aaaaccgtcg aaacaccgga tctgcgtacc gacctcgtct
     3301 atcgcctgcg tattgtggtg accgacgccg atgatgcgtt acgccaggga atgccggtaa
     3361 cggtgcaatt cggtgacgag gcaggacatg aatgatgccg ttatcacgct gaacggcctg
     3421 gaaaaacgct ttccgggcat ggacaagccc gccgtcgcac cgctcgactg caccattcac
     3481 gccggttatg tgacggggct ggttgggccg gacggtgcgg gcaaaaccac gctgatgcgg
     3541 atgctggcgg gattactgaa acccgacagc ggcagtgcca cggtgattgg ctttgatccg
     3601 atcaaaaatg acgctgcgct gcacgctgtg ctcggctata tgccgcagaa attcggcctg
     3661 tatgaagatc tgacggtgat ggaaaatctc aatctgtacg cggatttgcg cagcgtcacc
     3721 ggtgaagcac ggaagcagac ttttgctcgc ctgctggagt ttacatctct tggcccgttt
     3781 accggacgcc tggcgggcaa gctctccggt gggatgaaac aaaaacttgg cctggcctgt
     3841 accctggtgg gcgaaccgaa agtgttgctg ctcgatgaac ccggcgtcgg cgttgaccct
     3901 atctcacggc gcgaactgtg gcagatggtg catgagctgg cgggcgaagg gatgttaatc
     3961 ctctggagta cctcgtatct cgacgaagcc gagcagtgcc gtgacgtgtt actgatgaac
     4021 gaaggcgagt tgctgtatca gggggaacca aaagccctga ctcaaaccat ggccggacgc
     4081 agctttctga tgaccagccc gcacgagggc aaccgcaaac tgttgcaacg ggcattgaaa
     4141 ctgccgcagg tcagcgacgg catgattcag gggaaatcgg tacgtctgat cctcaaaaaa
     4201 gagaccacac cagacgatat tcgccatgcc gacgggatgc cagaaatcaa catcaacgaa
     4261 actacgccgc gttttgaaga tgcgtttatt gatttgctgg gcggtgccgg aacctcggaa
     4321 tcgccgctgg gtgcaatatt gcatacggta gaaggcacac ccggcgagac ggtgatcgaa
     4381 gcgaaagagc tgaccaaaaa gtttggcgat tttgccgcca ccgatcacgt caactttgcc
     4441 gttaaacgcg gggagatttt tggtttgctg ggaccaaacg gcgcgggtaa atcgaccacc
     4501 tttaagatga tgtgcggttt gctggtaccg acctctggtc aggcgctggt gctgggcatg
     4561 gatctgaaag agagttccgg taaagcgcgc cagcatctcg gctatatggc gcaaaaattt
     4621 tcgctctacg gcaacctgac ggtcgaacag aatttacgct ttttctctgg tgtgtatggc
     4681 ttacgcggtc gggcgcagaa cgaaaaaatc tcccgtatga gcgaggcgtt cggcctgaaa
     4741 agtatcgcct cccacgcgac cgatgaactg ccattaggtt ttaaacagcg gctggcgctg
     4801 gcctgttcgc tgatgcatga accggacatt ctgtttctcg acgaaccgac ttccggcgtt
     4861 gatcccctca cccgccgtga attctggctg cacatcaata gcatggtaga gaaaggcgtc
     4921 acggtgatgg tcaccaccca ctttatggat gaagcggaat attgcgaccg catcggcctg
     4981 gtgtaccgtg ggaaattaat cgccagcggc acgccggacg atttgaaagc gcagtcggct
     5041 aacgatgagc aacccgatcc cacaatggag caggccttta ttcagttgat ccacgactgg
     5101 gataaggagc atagcaatga gtaatccgat cctgtcctgg cgtcgcgtgc gggcgctgtg
     5161 cgtcaaagag acgcggcaga tcgttcgcga tccgagtagc tggctgattg cggtagtgat
     5221 cccactgcta ctgctgttta tttttggata cggcattaac ctcgactcca gcaagctgcg
     5281 cgtcgggatt ttactggaac agcgtagcga agcggcgctg gatttcaccc acaccatgac
     5341 cggttcgccc tacatcgacg ccaccatcag cgataaccgt caggaactga tcgccaaaat
     5401 gcaggcgggg aaaattcgcg gtctggtggt tattccggtg gatttcgccg aacagatgga
     5461 gcgcgccaac gccaccgcac cgattcaggt gatcaccgac ggcagtgagc cgaataccgc
     5521 taactttgtg caggggtatg tcgaagggat ctggcagatc tggcaaatgc agcgcgcgga
     5581 ggataacggg cagacttttg aacagcttat tgatgtacaa acccgctact ggtttaaccc
     5641 ggcggcgata agccagcact ttattatccc cggtgcggtg accattatca tgacggtcat
     5701 cggcgcgatt ctcacctcgc tggtggtggc gcgagaatgg gaacgcggca ccatggaggc
     5761 tttgctctct acggagatta cccgcacgga gctgctgctg tgtaagctga tcccttatta
     5821 ctttctcggg atgctggcga tgttgctgtg tatgctggtg tcggtgttta ttctcggcgt
     5881 gccgtatcgc gggtcgctgc tgattctgtt ttttatctcc agcctgtttt tactcagtac
     5941 cctggggatg gggctgctga tttccacgat tacccgcaac cagttcaacg ccgctcaggt
     6001 ggccctgaac gcggcttttc tgccgtcgat tatgctttcc ggctttatat ttcagataga
     6061 cagtatgccc gcggtgatcc gcgcggtgac gtacattatt cccgcacgtt atttcgtcag
     6121 caccctgcaa agcctgttcc tcgccgggaa tattccagtg gtgctggtgg tgaacgtgct
     6181 gtttttgatc gcttcggcgg tgatgtttat cggcctgacg tggctgaaaa ccaaacgtcg
     6241 gctggattag ggagaagagc atgtttcatc gcttatggac gttaatccgc aaagagttgc
     6301 agtcgctgct gcgcgaaccg caaacccgcg caattctgat tttacccgtg ctgattcagg
     6361 tgatcctgtt cccgttcgcc gccacgctgg aagtgaccaa cgccaccatc gccatctacg
     6421 atgaagataa cggcgagcat tcggtggaac ttactcaacg ttttgcccgc gccagcgcct
     6481 ttacccatgt gctgctgctg aaaagcccgc aggagatccg cccgaccatc gacacacaaa
     6541 aggcgttact actggtgcgt ttcccggctg acttctcgcg caaactggat accttccaga
     6601 ccgcgccgtt gcagttgatc ctcgacgggc gtaactccaa cagtgcgcaa attgccgcca
     6661 actacctgca acagatcgtc aaaaattatc agcaggagct gctggaagga aaaccgaaac
     6721 ccaacaacag cgagctggtg gtacgcaact ggtataaccc gaatctcgac tacaaatggt
     6781 ttgtagtgcc gtcgctgatc gccatgatca ccactatcgg cgtaatgatc gtcacttcac
     6841 tttctgtcgc ccgcgaacgt gaacaaggta cgctcgatca gctactggtt tcgccgctca
     6901 ccacctggca gatattcatc ggcaaagccg taccggcatt aattgtcgcc accttccagg
     6961 ccactattgt gctggcgatt ggtatctggg cgtatcaaat ccccttcgcc ggatcgctgg
     7021 cgctgttcta ctttacgatg gtgatttacg gtttatcgct ggtgggattc ggtctgttga
     7081 tttcatcact ctgttcaaca caacagcagg cgtttatcgg cgtgtttgtc tttatgatgc
     7141 ccgccattct cctttccggt tacgtttcgc cggtggaaaa catgccagtg tggctgcaaa
     7201 atctgacgtg gattaaccct attcgccact ttacggacat taccaagcag atttatttga
     7261 aggatgcgag tctggatatt gtgtggaata gtttgtggcc gctactggtg ataacggcca
     7321 cgacagggtc agcggcgtac gcgatgttta gacgtaaggt gatgtaactt cttatctttc
     7381 gccagcaaag acactaccgc agggccggca aggattgcca gccctgcaat tgccagcaca
     7441 aagttgtttt ccagtacgcg ggacaacagc cacagaacaa tcagcgctgc gccaaaatac
     7501 caggtcgtgg tcagctcttc cagcacgtct gccacctcac gccagcgttg ctcggggtgg
     7561 cgctgaaaca ccagcatcat cactaccgtg acacaataca gtgcgcataa tcctacgccg
     7621 acgtgaacca gagttttact catccagccc accaccagca gcgccactac cagtaaatgc
     7681 aacataatct gccagcaact taggccagtt gcgacacgaa cacgttgttg ccacttcatg
     7741 gcttctctcc tgaagaattt atctctactt attcagagta ccgaacttta cggaaaattc
     7801 cgtaagaccg cacctttttg tgacgccgac tacactattt caatgagata agaggcaaag
     7861 acaggaaaat tatgcccgat caaacacaac aattttcgtt caaggtgctc accatcaata
     7921 ttcacaaagg ctttacctcg tttaaccgac gcttcatttt gccggaactt cgcgacgccg
     7981 tgcgtaccgt cagcgccgat attgtttgcc tgcaggaagt gatgggcgcg cacgaagttc
     8041 atccgctgca tgtggaaaac tggcccgata cctcgcacta cgagtttctc gccgacacca
     8101 tgtggagcga tttcgcctac ggtcgcaatg ccgtataccc ggaaggacat cacggtaacg
     8161 ccgttctttc acgttatccc attgaacatt atgagaatcg tgatgtttcg gtcgatggtg
     8221 cggaaaagcg cggcgtgctc tactgccgca ttgtgccgcc gatgaccggg aaagcgattc
     8281 atgtgatgtg cgtacatctg ggcctgcgtg aggcgcaccg ccaggcgcag cttgcgatgc
     8341 tcgccgaatg ggtgaatgag ctaccggacg gcgaaccggt attggtagcg ggtgatttca
     8401 acgactggcg gcaaaaagct aatcatccgt taaaagtgca ggccgggctg gatgagattt
     8461 ttacccgcac ccacggacgc ccggcgcgca cgtttccggt gcaatttcct ctactacgac
     8521 tggacaggat ctacgtcaaa aatgccagcg ccagcgcgcc aaccgcgttg ccgctgcgga
     8581 catggcgaca cctttctgat catgcccctt taagtgcgga gattcattta tgaaatgtag
     8641 ctggcgcgaa ggcaataaga tccagttgct ggaaaacggc gagcaatatt atcccgcggt
     8701 gtttaaggcg attggcgagg cgcaagaacg catcattctt gaaacgttta tctggtttga
     8761 ggatgacgtc ggcaaacagc tgcatgcggc gctattggca gcagcgcaac gcggggtaaa
     8821 agcggaagtc ttgctggatg gctacggttc gccggatctc agcgatgagt ttgtcaatga
     8881 actgacggca gctggcgtgg tgttccgcta ctacgatccc cgccctcgcc tttttggtat
     8941 gcgcaccaat gtgtttcgcc ggatgcatcg caaaattgtg gtgatcgacg cgcgtatcgc
     9001 ctttattggc gggctgaatt actccgccga gcatatgtcc agctacggcc cggaggccaa
     9061 acaggattac gcggttcgcc tcgaagggcc gattgtcgaa gatattctcc agtttgagct
     9121 ggaaaacctg ccaggacaga gcgcggcgcg acgctggtgg cgacgtcatc ataaagcgga
     9181 agagaaccgc cagccgggag aagcgcaggt attgctggtc tggcgcgata acgaagaaca
     9241 tcgcgatgat atcgaacgtc actatctgaa aatgctcact caggcgcggc gggaagtgat
     9301 tatcgccaac gcctacttct tccccggcta tcgtttttta cacgccttgc gtaaagcggc
     9361 acggcgcggg gtgcggatca aactgatcat tcagggcgaa ccggatatgc cgattgtcag
     9421 agtcggcgca cgtttgctgt ataactatct ggttaaaggc ggcgttcagg tgtttgagta
     9481 ccgccgccgc ccgctccacg gcaaagtggc attgatggac gatcactggg cgacagtagg
     9541 gtccagtaat ctcgatccgc tcagtttgtc actgaatctc gaagcaaatg tcatcatcca
     9601 cgatcgtaat tttaaccaga cgctgcgcga taatctgaac ggcattatcg ccgcagattg
     9661 tcagcaggtc gatgagacca tgctgccgaa acgcacctgg tggaacctga ccaaaagcgt
     9721 gctggcgttc cactttttac gccacttccc ggcgctggtg ggctggcttc cggcgcacac
     9781 gccacgtctg gcgcaggttg gtccccccgc acaaccgaca atggaaacgc aggatcgggt
     9841 agaaactgaa aacacggggg taaaaccctg atgagtaaat cacacccgcg ctggcgctta
     9901 gcaaagaaac tcctcacctg gctgtttttt atcgcggtga ttgtgttgct ggtggtctac
     9961 gccaaaaaag tggactggga agaggtctgg aaggtcatcc gcgactacaa tcgcgttgcg
    10021 ctgcttagtg cggtcgggct ggtggtcgtc agttatctga tttacggttg ctacgacctg
    10081 ctcgcccgtt tctactgcgg tcacaaactg gcgaagcgcc aggtgatgct ggtgtcgttt
    10141 atctgctacg ccttcaacct gacactcagt acctgggtcg gcggcattgg tatgcgctat
    10201 cgtttgtact cccgactggg gttaccgggc agcactatta cgcggatttt ttcgctcagt
    10261 attaccacca actggctagg ctatattttg ctagcggggg ttatctttac cgcaggcgtg
    10321 gtggagttgc cggatcactg gtatgtcgat caaactacgc tgcgtattct cggtattggc
    10381 ttactgatga ttatcgcggt ttatttgtgg ttttgcgctt tcgcgaagca ccgccatatg
    10441 accatcaaag ggcaaaagct ggtgctgcct tcgtggaaat tcgccctcac ccagatgctg
    10501 atttccagcg ttaactggat ggtaatggga gcgattatct ggctgttact tggtcaaagc
    10561 gtgaactatt tctttgtact gggcgtgttg ctggttagta gtattgctgg cgtcatcgtg
    10621 catattccgg cgggaattgg tgtgctggaa gcggtgttta tcgcgctact ggctggggag
    10681 catacctcca aaggcacaat tatcgccgcc ctgctcgctt accgtgtgct gtattacttt
    10741 atcccactgc tgctggcgct gatctgttac ctgttgctgg aaagccaggc gaagaagcta
    10801 cgggcgaaaa atgaagcggc gatgtgaagt atagttaagt tctttagtat gtgcatttac
    10861 ggctaatgaa aaaaccgcgt atgcctttgc cagacaagcg ttatagctat agcggcaaga
    10921 atcccaaaga agccgataaa atagtaatag agtgttaatg cacctataaa agcacaacga
    10981 accgaactcc ataattcttc actatggctt tttcctgtca gttcaaggag cttatttcgt
    11041 ccatgcgaga taatgcctga ccataagaca atcattaagc aacttattac ccaaatgggt
    11101 cgttcactca ttaatgtggc attcacgatg attaccaggg ccaaccctgt caacgtcatc
    11161 ataatgataa aacggtgaga accaggatca aatttaaaca atagacctgc cagcatactg
    11221 attaaaaaca tcgatcccgc agtgctgaac acgacgataa tggcagatac aggcgctaaa
    11281 ctcgcaacga caatcgccac caaagagatt aaaaacaaat atattacgcc cgcgatggcg
    11341 gtatttatct tgttgatatc tttactcaag ccatacatag aaagtaataa taatcctata
    11401 gcaacataca tttgcggtga atcatatttt gttgccgtaa tttcgagata cttagcgccc
    11461 caggccagcc ctgcggttgc caccaaaccc aaagcggtaa tgagccatat gctgtttaat
    11521 atcctggctt cgtgctgaaa atctaattta ttgctatttt gcgagtatga ttccattctt
    11581 ttagttgtcc ttagaaaaaa cattaatatt caccatcaat aaatttaaaa ccacgcatac
    11641 aaaatacttt tgcatttatg gatacagaaa acgcttccgc tcgtccataa ggcatatttt
    11701 atacctggta cttgttaagt gtcgttttta gtaagaaaaa aaatcccgac acatggccgg
    11761 gatattgatg gtgaaaaaga attaacggcg gttgccgaag atccgcagca acatcaggaa
    11821 caggttgatg aagtccagat acaaggttaa cgcgccaaga atggaatatt tgcgcaggtt
    11881 cgacgtgtcg cgggtatcaa tctgctcacc catatttttc aatttctgag tgtcatacgc
    11941 cgtcaatccg acaaagacaa tcacgccgat gtaggtaact gcccacatca atgcttcgct
    12001 tttcagccag aagttgacca gcgatgccaa cacaatgccg attaacgcca taaacagcat
    12061 attgccaaag ccgcttagat cgcgcttcgt ggtgtaaccg tacaggctca ttgcgccaaa
    12121 catcccggca gtaacgacga aagtactggc gatagaagca gcggtataga caatgaaaat
    12181 actggaaagc gtaagacccg tcagcgccga ataaagcata aagagcatcg tcgtcacacc
    12241 tgcgctcagc ttttgaatca tcgctgataa cacaataacc aatgctaatt gcgcgatgat
    12301 cagaccgatt aaaaagacac ggttagtgaa caacagctcc atcacggccg cggagttagc
    12361 cgcataccag gcaacaaatg cggtcagcaa caagccaacg gtcatccagc catagacttg
    12421 cgccatataa gtttgcaagc cagcccgggg ttgtacaatt gaatcagaac gtgggaatct
    12481 gtccatgacg atctcctgaa gatataagga atatcttaag gatactgcaa aatgatgagg
    12541 ctgtgcatcg acgcagcgta aacgcatgta ctgagcggtg aaattgccgg acgcagcggt
    12601 gccttatccg gctaacaata aactaccagc gttttgccgc ctgctgatcg ctctcccgag
    12661 cttcgaccca gcggtcgcct tccggcgtgg cttcgcgctt ccagaacggt gcgcaggttt
    12721 tgagataatc cataataaac tgcccggctt caaacgcact gctgcgatgc gcactggtaa
    12781 caccgacaaa aacaatttca tcgcccggcc ataattcacc aatgcggtga attaccgtga
    12841 cgcgccccag cggccagcgg ttacgcgctt catcaacgat ttctgccagt gctttttctg
    12901 tcattcccgg atagtgttcg agggttaatg ctttgacgct gtcgcccaag ttatggttgc
    12961 gcaccttacc ggtaaaggtg actactgcac cgtcttcgtc acgctcagcc agccacgggt
    13021 actcttctcc tacgctgaac ggctgcgggc cgacaacaat tttggtttct gccatcttaa
    13081 cctccggtta ccggcgggaa gaaagccact tcgtcgccgt cagtcagcga atggtcaaaa
    13141 ctcaccagcg tctggttgac agcagccagc aatttgccat cttccagcgc cagcgcccag
    13201 cgatcgctct gcgcagccag atgttggcgt aacgcttcaa cggttgggaa atccgcagcc
    13261 acttcggttg catctgttcc caccaactcg cgcacctggg cgaaaaaaag aacgttaatc
    13321 atccgcttcc accttaaagt cacccgactt gccgccgctc tttgccagca aacgtaccgg
    13381 accaatcacc atatcttttt gcaccgcttt gcacatgtca taaatggtca gcgccgccac
    13441 ggaggccgcg gttaacgctt ccatttcgac accggttttc ccggtcaggc ggcataaggt
    13501 ttctatacgt acccgattgt gctccggctc ggcctgtaaa ttgacttcaa ctttgctgag
    13561 catcagcggg tgacacagcg ggatcagatc ccaggtgcgt tttgccgcct gaataccggc
    13621 gatacgcgca gtggcaaata cgtcgccttt gtggtggcga ccatcaataa tcatcgccag
    13681 cgtctcgctg cgcatggtga caaaggcttc ggcgcgcgct tcacgcacgg tttccgcttt
    13741 ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg atatgggtca gttgcgacat
    13801 acttatttct tcaaatgtgg atggaaatta cacggacgcg tacgggcatc cagctgtggc
    13861 gcaatgatat tttcccatgc ggtacggcac gctttggtcg aacccggcat ggcgaaaatc
    13921 agcgttttgt tagcgacgcc cgctaccgca cgagattgca acgtggaagt gccaatctct
    13981 tcgaacgaca acatccggaa cacttcacca aaaccttcaa cttcacggtc gaacaacggc
    14041 agcagcgctt cgggagcctg atcgccttcc gtcaggccag taccacccgt aatcaacacc
    14101 acttgtacat cgtcgctggc gatccacgca gatacctgag cgcgaatagc gtagcggttt
    14161 tctttcacaa tggctttatc gacaacgtga tggcccgctt cttgcgccga gtcgcgcaga
    14221 tagtgaccgg aggtatcgtc ttcttcaccg cgacgattag aaaccgtaag aatagcaata
    14281 cgggtcggga taaattcagt gcttacctga ctcatctgat ctctcctttt gacgttttag
    14341 ccaccaatgt acgataagtt ttgcgtaata ccggtgttat tttgatgcag gaaatgggtc
    14401 tgttttttct cccgcagcgc cgctgaaata cgcgcttcca gcgcctgttg ctgggtatcg
    14461 tcttccagca gatcgcgcag gttaacgccg ccttcaccaa acaggcagag atggagttta
    14521 ccaatggagg aaacgcgcag gcggttgcaa gtggcgcaga agtctttttc atacggcatg
    14581 ataaggccaa tctctccggc gtaatccgga tgacaaaaga cttgcgcggg accgtcgctg
    14641 cgttgacgta attggtggat ccagccgcga cgcagtagct cgtcacgcag aacctgacca
    14701 gagatgtgat gcttacggaa gagctcgatg ccctcgcccg tttccatcag ttcgatgaaa
    14761 cgcagctgga taggacgatg ctggatccag ttcagaaagg tgtcgagctg gtgatgatta
    14821 acatcacgca tcagcacggt attgactttg accttctcaa aaccggcctc aaatgcagca
    14881 tcgatccctg ccatgacctg gttgaattta tcctgcccgg taatggcatg aaactggcga
    14941 gcgtcaaggc tatcaacgct gacgttgatg ccagtaagtc cagcatcgcg ccagttcgcc
    15001 acatcgcgct ccagacggta accattggtg gtgaccgcta tctggcgaat agcgtcgttt
    15061 tctcgcacag cggcgatgat atcggtaaag tcgcggcgta aagatggctc accacctgtc
    15121 agacggactt tttcggtgcc cagactggcg aaggcgcgcg taacccggcg aatttcatcg
    15181 acggtcagaa agcctttatt ggtgacgccg ctcggcttgt aaccatccgg caggcagtag
    15241 gtgcaacgaa agttacacac atcagtaatc gacaggcgca agtagtaaaa cttacgcgca
    15301 aatgcatcag tcagttgtga agccatgtac acctttccag atacgggagg cgaagtcatt
    15361 tcttccttcg ccctggtggc aatctttacg agcattgtca cggccaaagc accgtatcag
    15421 ttgacccagg tgcagaggct agagtgttta gtggttaagc cgatactagc gtgtaaatga
    15481 gatttttacc atccacactt tcgctatgta atcatgtata tagcgtcatg atcgtgctat
    15541 ttcgctacag aataagggaa aatgacggtt ttactttgat atacatcatc agggaggcat
    15601 agcgtgcatc gtcctttaga ggtagaagaa aatagtttgt atcctcaata ttggcaggtt
    15661 aattgctgtt tcctcgcaat ttgcgctact gtagcgcgga taatctgact ccaggaaact
    15721 atatgcgcaa tcgtacgctg gctgaccttg atcgtgtcgt tgctctcggc ggagggcatg
    15781 gactgggacg cgttctctca tcactttcgt ctttgggttc tcgtttaacg ggtatcgtca
    15841 ccaccaccga taacggtggc tcgacggggc gtattcgccg ctcagaaggc ggcattgcct
    15901 ggggcgatat gcgcaactgc ctcaaccagc tgataactga acccagcgtc gcttccgcaa
    15961 tgtttgaata tcgtttcggc ggtaatggcg aactttccgg gcacaacctt ggaaacctga
    16021 tgctgaaggc actggatcac cttagcgtgc gacctcttga agcgatcaat ctgattcgaa
    16081 atctgctgaa agtggacgca catttgattc cgatgtcgga gcatccggtg gatctgatgg
    16141 cgattgacga tcaggggcat gaagtatacg gcgaggtcaa tatcgaccag ttaactacgc
    16201 cgattcaaga gttattgtta acgccgaatg tacccgcaac gcgtgaggcg gttcacgcta
    16261 tcagtgaagc ggatctcatc attattgggc ctggcagttt ttacaccagc ctgatgccaa
    16321 ttctgctgct gaaggaaatc gcacaggcat tacgccgcac gccagcgccg atggtctata
    16381 tcggcaatct ggggcgtgag ttgagtttac ctgcggctaa tctgaagctg gaaagcaagc
    16441 tggcaattat ggagcagtat gttggtaaaa aggtaattga tgcggtcatc gtcgggccaa
    16501 aagtggatgt ctcggcggtg aaagagcgga ttgtgattca ggaagtgctg gaggccagcg
    16561 atattccgta tcgtcatgac cgccagttgt tgcatagcgc gctggaaaag gcattacagg
    16621 ctttaggtta acacgaagcc ggaagaatat tccggcttcg tcatcttgtc acgacgcggc
    16681 aataaacagc tcacgcaact gatgcaactg gtcacgaatt tgcgccgctt cttcgaactc
    16741 cagattctgc gcgtgttgca tcatcaaccc ttccagttca tggatcttct gctgtaacgc
    16801 tttaggcgac atatccatcg gcacattatc cggctcaaca atcgggcgcg attttcctct
    16861 gcccttcgct ttggttttgg caatgttctg ccccagcgcc aggatatcga ccactttctt
    16921 gttcaagcct tgcggcgtaa tgccgtgttc ttcgttgtac ttctgctgtt tctcgcggcg
    16981 acgttcggtt tcgccaatcg ctttcgccat tgatggggtg atcttatcgc cgtagagaat
    17041 cgctttaccg ttaacgttac gtgccgcacg accaatggtc tggatcaacg aacgttcgga
    17101 acgcaggaag ccttctttgt cagcgtcgag gatcgccacc agcgaaactt ccggcatatc
    17161 cagaccttcg cgcagtaagt tgatccctac caatacgtcg aactcaccca gacgcaagtc
    17221 gcggataatc tccatacgtt cgacggtgtc gatatctgag tgaagataac gcacgcgctc
    17281 accgtgttct tcgagatatt cagtgagatc ttccgccatc cgcttggtca gagttgtaac
    17341 cagtacgcgt tcgttaattg ccgctcgctg acgaatctcc gaaagaagat catccacctg
    17401 tgttgccact ggccgcactt cgataatcgg gtcgagtaag cctgttggac gcaccacctg
    17461 atccaccaca tcgccaccgg atttttccag ctcgtaatta cccggcgtcg ccgaaacata
    17521 gatggtttgc ggcgctaatg cttcgaactc ttcaaatttc agcggacggt tatccagcgc
    17581 tgatggcagg cggaagccgt actccaccag tgtctcttta cgcgcccggt caccgcgata
    17641 cataccgcca atttgtggaa tggtgacgtg agattcatcg accaccagca gcccatcagc
    17701 aggcaggtaa tcaaacagcg tcggcggtgg ctcacccggt ccacgaccgg agaggaagcg
    17761 cgagtagttc tcaatccccg aacagtagcc tagctcgttc atcatctcca gatcaaactg
    17821 ggtacgctgg gtcagccgct gctcttccag cagtttgttg ttttccaata gcactttacg
    17881 tctggcagcc agctcttctt tgatctcctc catcgcctgg acgatgcgct cgcgtggcgt
    17941 gacgtagtgc gttttcgggt agatggtaaa acgtggaata gtggaaacaa tctgcccggt
    18001 cagcgggtca aataacgaca atcgttccac ctcctcgtca aacagctcca cgcgaagtgc
    18061 aatgtcatcc gattctgccg ggaagatatc aatcacctcg ccacgaacac ggaaagtacc
    18121 acgctggaat gcttgatcat tacgagcata ttgcagctcc gccagtcggc gcaggatcgc
    18181 gcgctgatcg ataatcatac cgaccgtgag atggagcatc atcttgagat ataaatcagg
    18241 atcgcccaga ccataaatcg cggaaaccga tgccaccaca accacatcac gccgctccag
    18301 catcgctttg gtggcggaca aacgcatctg ctcgatatgt tcgtttaccg aggcatcttt
    18361 ctcaatgaag gtgtcggaac tcggtacata ggcttccggc tgataatagt cgtagtagga
    18421 gacgaaatac tccaccgcgt tttccgggaa gaactctttc atttcgccat acagctgggc
    18481 cgccagcgtt ttgttgggcg caagtaccat ggtagggcgc tgaaggtcag caatgacatt
    18541 ggcaatggtg aaggttttcc cggagccagt cacgccaagt aacgtctggt gcgccaggcc
    18601 atcttccagc ccctcttcga gacgtcgaat cgcctccggc tgatcgccag aaggtttaaa
    18661 agcggaattc agtttgaacg gtttactcat gagtcgctac ctgaaggagt tgggcgggca
    18721 ggtatgtaat tttactcgtc gtatttaatt atgccaacaa attatactgg ataaaaaaac
    18781 agttcatcac cataatattt ctgttacagc gtaaactccg ctgtcaatca tgaacaagat
    18841 ttactctgtg gcgagataaa actccggcct taccgggtta tccccaaagc aacggctttt
    18901 ttaacatttg tcaagatgat tgatgacagt tttatggcaa gagatgcctg ttcagtgact
    18961 gccattgcaa ttatctaacc tgttaaaaaa taaaagatat ttctttgagc cgtctttaac
    19021 gccaattcgc gtgcaagccg cgtattctct cgcttgcctc gtgttttcta actctaatac
    19081 acatggttat ccacaggaat agtggataac tgtctgcagc ccctatcccc cgccgcctgg
    19141 gcaattccca cattcaccgc atgatgcgga ggtaaaattt ttttcgtgat ttttttagaa
    19201 tttaattggt taaattgcag tcaatcgaag acgcgatctc gctcacaatt taaccaagca
    19261 caggatgact acattaaggc aagattgatg tactttccgg ttgccgcatt ttctggattt
    19321 tctgcaagcc agggaatttc gcccagcagc ggcgcgggaa tcatgcgggt gagcgtggtc
    19381 atatattcag cgtgacgttt tcccggaggc gtgacatcgt tcgccaccca acccgccaga
    19441 gtcagtccgg cgtgtagtat tgcctgcgca gtcaacatcg cgtgattaat acagccgagt
    19501 ttcacaccaa ctaccagtat caacggcagt tgttcctgtg ttacccaatc cgcaaaagtg
    19561 aaagtgtcag aaagcggcgt aaaccagccg ccagcacctt ccactaacac ccagtcagcc
    19621 tgttgttcaa gcgcgcgtaa tccggcgctc attaccgatg attctatcgg cctgccctct
    19681 tgcgcgctga tgatgtgcgg cgaggtgggt tctgcgaagg tataaggatt taccgttgcg
    19741 taatccagct gcaggctact gatgcgctgt aacgccagcg cgtcactatt acgtaagcct
    19801 ttcggggtct tttcgctgcc agaggccacc ggtttataac ctgccgtccg gtagcctgct
    19861 gcctttgcgg cttgtaaaag cgcacagctg gcgacagttt tccccacttc ggtatccgtt
    19921 ccggtgacaa aataacattt actcacgagc aatcactccc aaaaaaagat gatacgtcag
    19981 aggatatcgc ccctgttgtt gcggccaggc cagttgcaat cgctgcaact gcgaacgcgt
    20041 taatatccgc gggtcgcgcc cttcatgaag atgcgtggca ccgatgcctt tcagcgaacg
    20101 catggcactg agcgcatcat caaaccacag cgtgatgggc tgaatatgat gttgataatg
    20161 caggccgttc agcgactgtt cgatttcatc tggcggtaaa aagcgattag catgcggacg
    20221 ctcgtccacc gcctgccacg cctgatgcag ttcgggtaac gatccctgca ccagcgtggt
    20281 aaacgcgacc acgcctccgg ggcgcaccac ccgatacagc tcgcggagtg ccgtggataa
    20341 attaccgcac cactgcaccg cgagattgct ccatgcaaga tcgaacgtcg cagtcgctaa
    20401 cggcagggat tcgatatctc ccgccagata atggtctgcg gcatccttct gacgtgcctg
    20461 aacaagcatt ggcggagaga gatctaacgc agtcacctgc gcgtgacgct ctcgccagcg
    20521 gcggctcatc cagcctggtc cacaacccgc atccagtacg tgggtgtatt tacgctgtgg
    20581 gagcattgcc agtaaggcgt cagcactctg gcgctgtaga tcggcgtgtt gctcatagtg
    20641 tgcggctgcc cgtccaaatg ccgcagcaat ggcttgttta ttaaccgttg ccatgcagca
    20701 cctccagcag acggtcgata tcctgcattt catgcgctgc ggttagcgtt aagcgcagtc
    20761 gcgcagtacc agcgggtacg gttggtgggc gaattgccgt gacccaacag ccttgctggc
    20821 gcagtttttc tgccagttgt aacgcacggc tgttatcacc gacgatcaat ggctggatgg
    20881 cgctgcatga gtcagcaagc gtaaacggca aatcctgcac tccggcacga aaacgagtaa
    20941 tgagtgccgc cagtttttcg cgccgtgcat ccccctcatc actgcgaatg accgccagcg
    21001 acgcacgtaa tgcctgcgcc tgagcgggcg gcatactggt gctgtagata agatggcgag
    21061 cgaattgcag cagataatcc gccacggtat cggagcaaag caccgctgcc ccgctgacgc
    21121 caaatccttt gccaaacgtc accaccagca attctggttt taccttttgc agccagcagc
    21181 tgccacgccc ctgttccccg ataacgcccg tgccgtgggc gtcatcgacc atcaaccagc
    21241 catcgtgctg ttgcgttact tgctggattt cctccagtgg cgcactatcg ccgtccatgc
    21301 tgaacacacc ttctgtcact accagttgct gccccggaca gggggaagca agcagtcgcg
    21361 ccagatgagt gacatcgtta tgagcaaaac ggcgaagcgg cgacgggctt aaacttgcag
    21421 cctccagcaa tgaggcatgg ctaagccggt cggcaacaat acggtcctct ttcgccatca
    21481 tcgcggcaat gacagcctga ttagcggcaa aaccagaaat aaacagcagt gcccgcgaat
    21541 agcccagcca ttcggccagt tcttcttcca gcacctggtg cgccacgcta taaccgctga
    21601 cgtgaccgga accgccgcta ccgacaccaa attgttccgc cccctgctgc caggcgcgga
    21661 taatttgcgg atgatggctt aaacctaaat aatcgttact ggaaaagttc agatactggc
    21721 aatcatctgc caccagccag cgtccggctc cttgtgccac cggataacgg cgacgcaagg
    21781 catcagcaac acgccgcgca tcgagcgccg cgtcgatttt ctcctgccag atcatagtgc
    21841 tgccgcgttg taatattcgt cagtgtccgg ggtcatcagc gcctgctcca gacgctgctg
    21901 ttgttcgtta tcgcccgcca gcacgacagt ttgctgcgga tttagcccca gtttgcggaa
    21961 cagttgcagg tctttatctt cttccggatt cggcgtagtc agcagtttgc aaccgtagaa
    22021 aatcgagttt gcgccagcca taaagcacat cgcctgagtc tgttcgttca tctgctcgcg
    22081 tccggcagaa aggcgtacgt aagaggtcgg catcatgatc cgcgctacgg caatggtacg
    22141 gataaaatca aaggcatcga catcgtcgtt atccgccagc ggcgtgcctt tcaccttcac
    22201 cagcatgttg attggcacgc tttccggcgg cgtcggcaag tttgccagtt gcagcaataa
    22261 tccggcgcga tctttcaccg tttcgcctaa gcccacaatg ccacctgaac agactttaat
    22321 cccagcttct cgcacttttt ccagcgtatc gagacgttcc tgataggtgc gggtggtgat
    22381 gatattgccg taaaactccg gcgaggtgtc gaggttgtgg ttgtagtaat ccagcccggc
    22441 attcgccagg cgctgcgcct gagattcact caacgtgccc agcgtcatac acgcctccag
    22501 ccccatcgct tttaccccct gcaccatttg ttccaggtac ggcatatcac gttcgtgggg
    22561 attcttccac gccgcgccca tacagaaacg tgtcgatccc gccgctttcg ctttgcgcgc
    22621 cgactccagc acctgttcaa cttccatcaa ccgctcggct tccagcccgg ttttgtagcg
    22681 cgagctttgc gggcaatatt tgcaatcttc cggacaagct ccggtcttaa tcgacagcaa
    22741 agtgctgacc tgcacctgac gaggatcgaa atgctgacga tgcacctgct gcgcttcaaa
    22801 cagcagatcc agcaacggtt tttcaaataa ttctgtgact tgcgacaatg tccagcgtgg
    22861 gcggtgagcc atggggcttc tccaaaacgt gttttttgtt gttaattcgg tgtagactcg
    22921 taaacctaaa tcttttcaat ttggtttaca agtcgattat gacaacggac gatcttgcct
    22981 ttgaccaacg ccatatctgg cacccataca catccatgac ctccccgcta tcggtttatc
    23041 cggtggcgag cgccgaaggt tgcgagctaa ttttgtctga cggcagacgc ctggttgacg
    23101 gtatgtcgtc ctggtgggcg gcgatccacg gctacaatca cccgcagctt aatgcggcga
    23161 tgaagtcgca aattgatgcc atgtcgcatg tgatgtttgg cggtatcact catgcgccag
    23221 ccattgagct gtgccgcaaa ctggtggcga tgacgccgca accgctggaa tgcgtttttc
    23281 tcgcggactc cggctccgta gcggtagaag tggcgatgaa aatggcgttg cagtactggc
    23341 aggccaaagg cgaagcgcgc cagcgttttc tgaccttccg caatggttat catggcgata
    23401 cctttggcgc gatgtctgtg tgcgatccgg acaactcaat gcacagcctg tggaaaggct
    23461 atctgccaga aaacttgttt gctcccgccc cgcaaagccg catggatggt gaatgggatg
    23521 aacgcgatat ggtgggcttt gcccgcctga tggcggcaca tcgtcatgaa atcgccgccg
    23581 tgatcattga accgattgtc cagggcgcag gcgggatgcg catctaccat ccggaatggt
    23641 taaaacgaat ccgcaaaatg tgcgatcgcg aaggtatctt gctgattgct gacgagatcg
    23701 ccaccggatt tggtcgtacc ggcaaactgt ttgcctgtga acatgcagat atcgcgccgg
    23761 acattttgtg cctcggtaaa gccttaaccg gcggtacaat gaccctttcc gccacgctca
    23821 ccacgcgcga ggttgcggaa accatcagca acggcgaagc cggttgcttt atgcatgggc
    23881 caacttttat gggtaatccg ctagcctgcg cggcagcaaa cgccagcctg gcgattctcg
    23941 aatctggcga ctggcagcag caggtggcgg ctattgaagt acaactgcgc gagcaactgg
    24001 caccagcccg tgacgccgaa atggttgccg atgtgcgcgt actgggggca atcggcgtgg
    24061 tcgaaaccac tcgtccggtg aatatggcag cgctgcaaaa attctttgtc gaacagggtg
    24121 tctggatccg gccttttggc aaactgattt acctgatgcc gccctatatt attctcccgc
    24181 aacagttgca gcgtctgacc gcaacggtta accgtgcggt gcaggatgaa acattttttt
    24241 gccaataacg ggaagtcagc gtgagggttt ctggctacac tttctgcaaa caagaaagga
    24301 gggctcttga cggctccccg ccctgaatga cggggattcc cacttcaacg agccgaacct
    24361 aagccgcgtg ctgcgattca gggtttacag gctctccgtg ggctaacact gccagcccgg
    24421 cagctttaat attacgtgcc gcgttaatgt cgcgatcatg gtctgcgccg cattcagggc
    24481 agtgccattt acgaacatta agaggcattt tttgcatggt gaaaccgcag caactacagc
    24541 attttgagga cggtaaatac tggtcaatgg cgaccactga ccgcccggcc catttgcctt
    24601 tgtactggag ctggcgaaca agttcgctcc agcctgcgtc agctattgct ttagacagct
    24661 tcgggttgcg gatcatgttt ttcaccttga gggattcgac gcaaacaact tggttttcgt
    24721 taatcagttt gcgggacaac ttgtgcagat tgtccatccg gcaatcggcg attttcgcgt
    24781 ggagtcgggc gacctttaag cgggctttaa tacggtttct tgagcctttt tgcttcctgc
    24841 ttaaacgtcg ctgtagcagc gttaatcgct tcgcatattt agcggtgtgg cggggattgt
    24901 cggttttgaa tccggtatcg gtgacgaata aatcttttaa gcccacatca atgccgaccg
    24961 ttttagcggt aacaggcatt gatacaggtt caaactcaca caggcaggaa acaaagtacc
    25021 tgccagcgct atctctggaa atggtaacgg ttgacggcgc agatggtaat tctcgactcc
    25081 agcgaacatc cagcggcgac ttgctctttg ctatatacaa ctcgccgtca cggtgtttaa
    25141 acgcgctggc agtgaactca gccacctgtt tgtgccgttt gcttttgaaa gccggatatg
    25201 cagctcgtcc ggcaaagaag ttagcaaagg cggcttgttg gtggcgcaac gactgctgga
    25261 ggggaacgca ggaaacatca ttcagccata tgtattcagg ctcttttttg agcgccgtaa
    25321 ggcgagcgtt ggcctgtaga taaccgatct tttcttttcg ctcgtagtac gcatcggtac
    25381 gccaacgaag gatggaattg tagacgaagc gcacacagcc aaacgtctga gctaaaagct
    25441 cagcctgctc agttgtcggg taaaaccggt atttatatgc gcgtttcatg tgttcacata
    25501 ctaaagagga aaatgtgatt atgcaaagta cagttagtcg gaaaaccgcc tcctttcctc
    25561 cccggtctga aggccgaggt ttcccggagg cattctaatg aaactcatca gtaacgatct
    25621 gcgcgatggc gataagctgc cgcatcgtca tgtctttaac ggcatgggtt acgatggcga
    25681 taatatttca ccgcatctgg cgtgggatga tgttcctgtg ggaacgaaaa gttttgttgt
    25741 cacctgctat gacccggatg cgccaaccgg ctccggctgg tggcactggg tagttgttaa
    25801 tttacccgct gatacccgcg tattaccgca agggtttggc tctggtctgg tagctatgcc
    25861 agacggcgtt ttgcagacgc gtaccgactt tggtaaaacc gggtacgatg gcgcagcgcc
    25921 gccgaaaggc gaaacccatc gctacatttt taccgttcac gcgctggatg tagaacgtat
    25981 tgatgtcgat gaaggtgcca gcggcgcgat ggtcgggttt aacgttcatt ttcactctct
    26041 ggcgagtgcc tcgattactg cgatgtttag ttaatcactc tgccagatgg cgcaatgcca
    26101 tctggtatca cttaaaggta ttaaaaacaa ctttttgtct ttttaccttc ccgtttcgct
    26161 caagttagta taaaaaagca ggcttcaacg gattcatttt tctatttcat agcccggagc
    26221 aacctgtgaa cacattttca gtttcccgtc tggcgctggc attggctttt ggcgtgacgc
    26281 tgaccgcctg tagctcaaca ccgcccgatc aacgtccttc tgatcaaacc gcgcctggta
    26341 cctcttcgcg cccgattctg tcggcaaaag aagcgcagaa tttcgatgct caacactatt
    26401 ttgcatccct gacaccaggt gcggcagcgt ggaatccttc cccgattacc ctgcctgcgc
    26461 aacctgactt tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc acgattcagg
    26521 cggcggtaga tgcggcaatt atcaagcgta ccaacaagcg ccagtatatt gccgtgatgc
    26581 ctggtgagta tcagggaacg gtgtatgtcc ctgccgctcc gggtggaatt actctgtacg
    26641 gtacaggtga aaaaccgatt gatgtgaaga ttgggctttc ccttgatggt ggcatgagcc
    26701 ctgccgactg gcgtcacgac gtcaacccgc gcggcaaata tatgccaggt aaaccggcgt
    26761 ggtatatgta cgatagctgc cagagtaaac gcagcgacag tatcggtgtt ctctgctctg
    26821 cggtcttctg gtcacaaaac aatggcctgc aactgcaaaa cctgaccatc gaaaacacgc
    26881 tgggcgatag cgtagatgcg ggtaaccatc cggcggtggc actgcgtact gatggcgaca
    26941 aagtgcagat caataacgtc aacattctcg gtcgtcagaa caccttcttt gtcaccaaca
    27001 gcggtgtgca gaaccgtctg gaaacgaatc gccagccgcg tacgctggtg accaacagct
    27061 atattgaagg ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc gataacaccg
    27121 aattccgcgt ggtgaactcc cgtacccagc aagaagcgta tgtgtttgca ccggctacgc
    27181 tgtccaacat ttactacggt ttcctcgccg taaacagccg tttcaatgct tccggtgatg
    27241 gcgtggcgca actgggccgc tcgctggatg ttgatgccaa taccaacggt caggtagtga
    27301 tccgtgatag cgccatcaac gaaggtttta acacagccaa accgtgggct gatgcggtga
    27361 tctctaatcg tccgtttgcg ggtaacaccg gcagcgttga tgataacgac gaagtacagc
    27421 gcaatctgaa tgacactaac tacaaccgca tgtgggaata caataaccgc ggcgtgggta
    27481 gcaaagtggt tgcagaggcg aagaagtagg agctgttaac tatgtgccgg atgcggcgta
    27541 aacgccttat ccggcctaca gttcgatacg atttgtcggt cggataagat gcgtcggcat
    27601 cgcatccgac aataactgcg gatgcgacct acattcacat ggcgcttttt acatctgacg
    27661 gtttttattg aagttaatca aactacccgc cttgataatc tcgcgttctt cggcagtcag
    27721 actttccata tacagcgtga tttccgttac cggtgcatct tcatggatca cataaccttt
    27781 aaacgtcgtt ccaggattat ccagcgccgc tttaatgcca gggatgtaaa tgtaatcccc
    27841 cacttcaaag gttggcgctt ccgccatctg cagcggtaac atcccccagt tgatgacgtt
    27901 agaacgatag cgtttagtcg cgtactcctc ggcaatattc gccagaccgc caatcacacg
    27961 ctgacaactc gccgcctgtt cacgcgcaga accatcgcct ggtttcaccg catagaccat
    28021 actgccaatt tcagtttgca gcggatcaat atgctcctga ccagcaatct gcttaatgcg
    28081 cgcaaacaca tctgccatct cgctgacatt ccccgccaga cgctgatttt ccagctcagc
    28141 agtcgcttta cttctgccaa cataacctgg atcgcggcgt gacagggtaa actccgccag
    28201 accaatcgga tttgaacgat aagaagaggt ttcaccggaa ggaatcagtt cgtcggtggt
    28261 ggtcacttcg tcgaggatct tcgagcacac tttgaggacg atattgtcag tcagcgcacc
    28321 caattccggc cagtctttaa tgttcggtcc gtaaatcagc ggttgctggg tcgccccttt
    28381 cacaaagccc tgataaacac ggtttttata cggcgttaca tcgaaggcgt actccggcac
    28441 gttgtcccag caatcgagtt cgctggcaga ggttaaatag ccaccgtttg ccgcagtcgc
    28501 agcgatagaa cgagcgtcca tcaacgccac cgctgacatc tgcccattag ctggcttaga
    28561 gccttcgcgg ttcgggaagt tacgcgtggt gtggcgaata ctcaaaccgt tgttgattgg
    28621 cgtatcgccc gcgccaaagc atgggccgca gaacgcggtt ctgatgattg cgcctgcgcc
    28681 aatcaaatct gcgaccacac cttttttggc gagatccata aacaccggct gtgatgacgg
    28741 gtaaaccgcc aacgagaagg tatcgttgcc gcaggattga ccacgcagcg cattcgccgc
    28801 cgcgatgacg ttttcatagt taccgccaga acagcccgcg ataatcccct gctgcacttt
    28861 caggcgacca ttttccactt tatccagcag cgagagtttg gctttaccgt gcgccacgcg
    28921 ttcggactca atttcaatct cacgcagaat gtcggtcagg ttctggttca gcgtgtcgat
    28981 ttcatacacg ttgctcgggt ggaatggcag cgcaatcatt ggtttgatgg cgcttaaatc
    29041 aacgctgatg cagccatcgt agtacgccat tggttgaggg ttaagttggc agtaatcctg
    29101 gccgcgaccg tgcagcgcca gccagttatg gacttcttca tcggtttgcc agacagaact
    29161 taaacaggtc gtttcagtgg tcatcacgtc aacgctgtta cggaaatcgg tagagagcgc
    29221 tgcaacaccg ggacctacga actccatcac tttgtttttg acgtaaccgt ttttgaacac
    29281 agcaccgatg atagccagcg ccacatcctg cggccccaca tacggcgcag gttttccggt
    29341 cagatgcacc gcaaccacgc ccggatagtc gatatcccag gtgtcattaa gcagctgttt
    29401 taccaactcg ccgccgccct cgccaaccgc cattgtccct aacgcaccgt aacgggtatg
    29461 gctgtctgac ccgaggatca ttttgccgcc gcctgccatc atttcacgca tatattgatg
    29521 gatgaccgca atatgcggtg gcacaaaaat accgccataa cgctgcgccg ccgataaacc
    29581 aaaaacatgg tcatcaccgt taatggtgcc gccgacggcg cagagtgagt tatggcagtt
    29641 ggtcagcaca tacggcagcg ggaaacgttc cataccggac gctttagcgg tctgtacaat
    29701 accaacaaag gtaatatcgt gagaggctaa tgaatcaaac ttaattttaa gtttatccat
    29761 atttccggac gtattatgag aagagagaat agaccaggca atagtgcctt tttttgcttc
    29821 ttcttttttt atttcgccgg tgaaatgttc ttcggcaatt atttcgttat tgctggcgag
    29881 aaacacgcct ttttcagata acttgatcat caaatgctcc agatatctgc gcaccgtgaa
    29941 gtgcgcagaa agataataaa tg
//
LOCUS       DADTFG010000049        29508 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.049, whole genome shotgun sequence.
ACCESSION   DADTFG010000049 DADTFG010000000
VERSION     DADTFG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.049"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(169..1560)
                     /gene="narK"
                     /locus_tag="J1138_001996"
     CDS             complement(169..1560)
                     /gene="narK"
                     /locus_tag="J1138_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBA8453799.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPLLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            1899..3695
                     /gene="narX"
                     /locus_tag="J1138_001997"
     CDS             1899..3695
                     /gene="narX"
                     /locus_tag="J1138_001997"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBA8453800.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            3688..4338
                     /gene="narL"
                     /locus_tag="J1138_001998"
     CDS             3688..4338
                     /gene="narL"
                     /locus_tag="J1138_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBA8453801.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLVTLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(4339..5733)
                     /gene="ychO"
                     /locus_tag="J1138_001999"
     CDS             complement(4339..5733)
                     /gene="ychO"
                     /locus_tag="J1138_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBA8453802.1"
                     /translation="MSRFIPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALEKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMVRGYDLTARMRMPFYQHFNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            5918..6271
                     /gene="ychN"
                     /locus_tag="J1138_002000"
     CDS             5918..6271
                     /gene="ychN"
                     /locus_tag="J1138_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBA8453803.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(6315..7010)
                     /locus_tag="J1138_002001"
     CDS             complement(6315..7010)
                     /locus_tag="J1138_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBA8453804.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNELLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(7168..7398)
                     /gene="chaB"
                     /locus_tag="J1138_002002"
     CDS             complement(7168..7398)
                     /gene="chaB"
                     /locus_tag="J1138_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBA8453805.1"
                     /translation="MPYKTKSDLPESVKHVLPSHVQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            7668..8768
                     /gene="chaA"
                     /locus_tag="J1138_002003"
     CDS             7668..8768
                     /gene="chaA"
                     /locus_tag="J1138_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBA8453806.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            9173..9280
                     /locus_tag="J1138_002004"
     CDS             9173..9280
                     /locus_tag="J1138_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrB"
                     /protein_id="HBA8453807.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(9429..10283)
                     /gene="kdsA"
                     /locus_tag="J1138_002005"
     CDS             complement(9429..10283)
                     /gene="kdsA"
                     /locus_tag="J1138_002005"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBA8453808.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNLPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(10319..11128)
                     /locus_tag="J1138_002006"
     CDS             complement(10319..11128)
                     /locus_tag="J1138_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBA8453809.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(11132..11524)
                     /locus_tag="J1138_002007"
     CDS             complement(11132..11524)
                     /locus_tag="J1138_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBA8453810.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(11521..12354)
                     /gene="prmC"
                     /locus_tag="J1138_002008"
     CDS             complement(11521..12354)
                     /gene="prmC"
                     /locus_tag="J1138_002008"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBA8453811.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIFAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(12354..13436)
                     /gene="prfA"
                     /locus_tag="J1138_002009"
     CDS             complement(12354..13436)
                     /gene="prfA"
                     /locus_tag="J1138_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBA8453812.1"
                     /translation="MKSSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(13478..14734)
                     /gene="hemA"
                     /locus_tag="J1138_002010"
     CDS             complement(13478..14734)
                     /gene="hemA"
                     /locus_tag="J1138_002010"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBA8453813.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQIFGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            14948..15571
                     /gene="lolB"
                     /locus_tag="J1138_002011"
     CDS             14948..15571
                     /gene="lolB"
                     /locus_tag="J1138_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBA8453814.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            15571..16422
                     /gene="ispE"
                     /locus_tag="J1138_002012"
     CDS             15571..16422
                     /gene="ispE"
                     /locus_tag="J1138_002012"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBA8453815.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            16573..17520
                     /locus_tag="J1138_002013"
     CDS             16573..17520
                     /locus_tag="J1138_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBA8453816.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            17645..19324
                     /gene="dauA"
                     /locus_tag="J1138_002014"
     CDS             17645..19324
                     /gene="dauA"
                     /locus_tag="J1138_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBA8453817.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(19379..19657)
                     /gene="ychH"
                     /locus_tag="J1138_002015"
     CDS             complement(19379..19657)
                     /gene="ychH"
                     /locus_tag="J1138_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBA8453818.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            19935..20519
                     /gene="pth"
                     /locus_tag="J1138_002016"
     CDS             19935..20519
                     /gene="pth"
                     /locus_tag="J1138_002016"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBA8453819.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            20636..21727
                     /gene="ychF"
                     /locus_tag="J1138_002017"
     CDS             20636..21727
                     /gene="ychF"
                     /locus_tag="J1138_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBA8453820.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            21865..22095
                     /locus_tag="J1138_002018"
     CDS             21865..22095
                     /locus_tag="J1138_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453821.1"
                     /translation="MQLLCDNNHSALTVQEFVMKKTFTFSLLAIVFASLLSACVPHHH
                     HRHSDGPRGPAPSGKPMPPSNGGPNHGSHGRY"
     gene            complement(22132..24060)
                     /gene="dhaR"
                     /locus_tag="J1138_002019"
     CDS             complement(22132..24060)
                     /gene="dhaR"
                     /locus_tag="J1138_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBA8453822.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSV
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRG"
     gene            24279..25349
                     /gene="dhaK"
                     /locus_tag="J1138_002020"
     CDS             24279..25349
                     /gene="dhaK"
                     /locus_tag="J1138_002020"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBA8453823.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWHEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            25360..25992
                     /gene="dhaL"
                     /locus_tag="J1138_002021"
     CDS             25360..25992
                     /gene="dhaL"
                     /locus_tag="J1138_002021"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBA8453824.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLPVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            26003..27421
                     /gene="dhaM"
                     /locus_tag="J1138_002022"
     CDS             26003..27421
                     /gene="dhaM"
                     /locus_tag="J1138_002022"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBA8453825.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPHNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            27741..29438
                     /locus_tag="J1138_002023"
     CDS             27741..29438
                     /locus_tag="J1138_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBA8453826.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKIFADAVPNSDPLMILADYRMQQHQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAIGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT         7331 a         7638 c         7328 g         7211 t
ORIGIN      
        1 agatttttat gcagatgggt attaaggagt attccccatg ggtaacatat taatgactct
       61 taatgaaatc cagcgaaata agccatacaa cattagtatg ttgtaactaa atgacgcata
      121 aaaaaagcgc gacatcatgc cgcgcaaagg ataatcaaat attacgattt attttttaga
      181 atgccgacca tataccgccc aggtaatcac cacgcaggcg atatagaaaa tcaaaaatac
      241 tttcattgcg ccgactggcg aacccgttaa tgccagcgag ctaccaaacg ctttcgggat
      301 aaagaagcca ccaatcgcgc caatcgcaga gatgaaaccc agcgccgctg ccgtgtcggt
      361 tgccgcttca cgcatcgcac gttcgtcaga acccccttct gctttcacgc gatccattgt
      421 cagtttacgg aagatcacgg aaatcatctg gaaagtggaa ccactaccca gcccagcggt
      481 caggaacagc gccaggaaga ctgcgaagaa cgccatgaag cttccgccct gtccgtcagt
      541 cggtaaggtc aggaacagca ggccgctgaa aatcgccatc aggataaagt tcaccagcgt
      601 gacacgagtt ccgcccagac ggtcagataa tgcaccacct gcagaacgcg ccagcgcacc
      661 aataaacggc ccgaagaaag cgtattgcag aatctgaaca tccgggaact gcgtttttga
      721 cagcatcgca aagcccgcgg agaagccgat gaaggagccg aaggttgcca gatacagcag
      781 gctcataatc cacagatgac cccgtttgag taccggcaac tgctccttga tggaggcttt
      841 cgaggtggca agatcgttca tgccaaacca cgccgcaatg gtgaagatgg caagtaacgg
      901 cacccatatc caggacgcat tcgccagata cagctcagtc ccatccggct gtttgacgcc
      961 ctggctacca aatactgcga aaatcgacag tgataccacc agcggagcaa ccaactgcat
     1021 gacgctgacg cccatgttgc ccagaccccc attcagaccc agcgcgccac cctgcttctg
     1081 tttcggaaag aagaagctga tgtttgccat actggatgcg aagttcgcgc cagcaaagcc
     1141 gcacagcaga gaaatgatga tgaagacgct ataaggcgtg gaggtatcct gcacggcaaa
     1201 acccagccag acgcaaggaa taatcagaat accggtgctg aacgccgtcc agcgacgacc
     1261 accgaagata ggaaccataa aggagtatgg aacacgtaat aacgcgccag aaaccgaagg
     1321 cagcgcagtc aacataaata gctgatcggt cgtaaaatta aaaccgactt tcggtaagtt
     1381 caccgcaaca gcgctgaaca acatccatac gcaaaacgcc agcagcagac agggaacgga
     1441 aatccacagg ttgcggctgg caatacgttg accgcgttgt tgccagaacg caggatcttc
     1501 cggtcgccaa tctgtaatga cagctccagt agccctttcg ggggcggatg agtgactcat
     1561 agacacctct gatacacgtt tcgatgtctg ccaccttagt gtctgtagct aaaggcaatt
     1621 tgatgtaaat caaacgataa gataacttta tctatcattg atatttatca ttatccatag
     1681 tgagtacagt gatttcataa aaattatgag atttttcacg gtactgtaaa atccctaccc
     1741 ttaccgatgt aaagcgacta accacacggc aaataaggag taactctttc cgggtatggg
     1801 tatacttcag ccaatagccg agaatactgc cattccagaa tgtatcgtca cattcattaa
     1861 ggttattgct catttaaagc ctgaaggaag aggtttacat gcttaaacgt tgtctctctc
     1921 cgctcaccct ggttaatcag gttgcgctta ttgtgttgct ttctactgct attggactgg
     1981 cagggatggc ggtttctggc tggctggtgc aaggcgttca gggcagcgcc catgcgatca
     2041 acaaagcggg atcgctgcgc atgcaaagtt accgtctgtt ggcagcagtg ccattaagcg
     2101 agaaagacaa gcccttaatt aaagagatgg aacaaacagc atttagcgcc gagttgactc
     2161 gagcagcaga acgagacggg caactggcgc aattacaggg tttacaagat tactggcgta
     2221 atgaactgat ccctgcactg atacgtgcac aaaaccgcga aacggtgtca gcggatgtca
     2281 gccagtttgt tgccgggctt gatcagctgg tatctggttt tgatcgcacc acggaaatgc
     2341 gcattgagac agtggtgctg gtccatcggg taatggcggt atttatggca cttttactgg
     2401 tattcactat tatctggttg cgggcgagat tgctacaacc gtggcggcaa ctgctggcaa
     2461 tggcgagtgc cgtcagccat cgcgatttta cccaacgcgc aaacatcagc gggcgcaacg
     2521 aaatggcgat gcttggaact gcgttgaaca atatgtctgc agaactggcc gaaagttatg
     2581 ccgtacttga gcagcgggtt caggagaaaa ccgccgggct ggagcataaa aatcagatcc
     2641 tctctttttt atggcaggcc aaccgccgat tgcattcccg cgccccgctg tgtgaacgcc
     2701 tgtcacctgt actcaacgga ttacagaatt taaccctgct acgtgatatc gaactgcggg
     2761 tgtatgacac tgatgatgaa gagaatcatc aggagtttac ctgccagcca gatatgactt
     2821 gtgatgataa aggctgccag ctctgcccgc gcggcgtatt acccgttggc gatcgcggca
     2881 caaccctgaa gtggcggctg gctgacactc atacgcagta cggtattttg ctggcgaccc
     2941 tgccgcaggg gcgtcatctt agccatgatc aacaacaact ggtggatacc ctggttgaac
     3001 aactcaccgc cacgctggcg ctggatcgcc atcaggaacg tcagcaacag ttgatcgtga
     3061 tggaagagcg tgccaccatt gcgcgcgaac tgcatgattc tattgcccaa tctctctctt
     3121 gcatgaagat gcaggtgagt tgcttacaga tgcagggcga tgcgctgcca gaaagcagcc
     3181 gcgaactgtt aagtcagatc cgtaacgaac tgaatgcatc ctgggtgcag ttgcgtgaat
     3241 tgctcaccac attccgtttg cagctcaccg agcctggatt acgtccggcg ctggaggcga
     3301 gttgcgaaga gtacagcgcc aaatttggct tcccggtgaa gctggattat caattgccgc
     3361 ctcgtctggt gccttcgcat caggcaatcc acttgttgca aattgcccgt gaggcattaa
     3421 gtaacgccct caaacattcg caagcgagtg aggtcgtggt gacggtggcg caaaacgata
     3481 atcaggtcaa actgaccgtc caggataacg gctgcggcgt gcctgaaaat gccatccgca
     3541 gcaatcacta cggcatgata ataatgcgcg accgtgcgca aagtttacga ggcgattgcc
     3601 gcgtccgccg tcgtgaatca ggtggcaccg aagtggtggt cacctttatt cccgaaaaaa
     3661 ctttcacaga cgtccaagga gatacccatg agtaatcagg aaccggctac tatcctgctg
     3721 atcgacgatc acccgatgtt gcgaactggc gtaaaacagc ttatcagtat ggcaccagat
     3781 atcaccgtgg ttggcgaagc gagtaatggc gaacagggta ttgaactggc ggagtctctt
     3841 gatcccgatc tgatcctgtt agatctcaat atgcccggca tgaacggtct ggtaacgctg
     3901 gataaactgc gcgaaaagtc cctctccggt cgcattgtgg tattcagcgt ctctaaccat
     3961 gaagaagatg tggtcaccgc actgaaacgc ggcgcggatg gctatctgtt aaaagatatg
     4021 gaaccggaag atctgctgaa agcgttacat caggcagctg ctggcgaaat ggtattgagc
     4081 gaagcattaa cgcctgttct ggccgccagc ttgcgcgcta accgtgccac tactgagcgc
     4141 gatgttaacc agttaacccc acgcgagcgc gatattctca agctgattgc ccagggtttg
     4201 ccgaacaaga tgattgcccg ccgcctggac atcaccgaaa gcacagtaaa agttcacgtc
     4261 aagcacatgc tgaagaaaat gaagctaaag tctcgcgtgg aagctgcggt atgggtgcat
     4321 caggagcgca ttttctgatt acggttccca gcgcagttcg tcgtttgaca atgcgtcgaa
     4381 cggttcgaca agcgttagcg tgatctcatt ggaggagaca cgctgcccct ggttatcttc
     4441 caccaccact gacaatcgcc agtgattgct tgcgccttcc ccgttttgcc agtcaggcat
     4501 gatcagcgtc cagccctcct cactgttggc ttgtgcgccc ggcgtcaaac ttaatatctg
     4561 cgtatcgccc tgccaaatca gttgccgaat accgtaacga ctgcggattt gtaatttcag
     4621 cggcactgtt tcgccaggtt ttagatccca cggcggtgtc gccagaaaca ccgttaacgt
     4681 ttttcgctgt cggtactcaa gagtcggaag attatttcgc tgcggattgt catagcgact
     4741 accacgtaac gactgacttt cgacaacctc gcccgcagaa agttgttttt tgagcggtac
     4801 accaaagcgg tagttaagat tcagcccgag gttattttga ttctcgccac tttcaccctg
     4861 tttatgctgg gccgtcacag tgactaaggg cacaggggtg taatttaatc ccagactcaa
     4921 cgcgacggga ttgtgataac ccgtaccaga gttaaacaaa tcgacacgat caccaaaata
     4981 ctgttctacg ctgacactgg tattgaagtg ttgatagaac ggcatgcgca tccgggctgt
     5041 caggtcgtac ccgcgcacca tccgttgttc ctgcgtggct gtctgttcat gccatgcagc
     5101 aaacggctga taaaagtttg ccgatagtcg caaatattcg ccccacgctt cagcgccaaa
     5161 gcccgctcgc tgaagatttt cgtccagcaa gttgtcataa aaagtgttat aacccaccag
     5221 ccagctgccg cgcgcccagc gttgcccaac gcccacattg ctcaccagcc catcatcctg
     5281 ctgagtaaga ccaagctggc tccaggtgag ataacgatca ttatcttgta acggcacaaa
     5341 ccagcttcca cgactgccgg tgaaatgtcc ttcgttatcc actttgacgt caacactggc
     5401 atttccccac ggtgatagcc aggactctac gtgctgatta acctgttgac taagcgcgtc
     5461 gcggaccttt tccaatgcga aagcttttgc ctgctcgcca gtatcaagcc cgttatcatt
     5521 catactggtt tcgccgaaat ctttcacaat ttcagcaaag tgtttttccc catcatgatt
     5581 ttcaggtgcc atgcctaaat ccggcagacc atcattgtta ttatcaaagg gatttgccgc
     5641 tttttgctcg aaggtagatt gtgcgttagc tgtaccgcct gccgccagca agagtaaata
     5701 aaacggaata atgcgaggaa tgaaacggct caaaacgtcg gtaacttccg gtaaaaatga
     5761 aaaaccatag ccttaaagaa taacgattac tggcaccttt tgtcgtattt tctcgcgctt
     5821 tcaggaaaat ccttaaaaat gtgaccgatt ggtcagacgc tgcagaagca gtaagataga
     5881 agatgagtgt ctaattccct gaatacagga gtaaataatg caaaaaatcg tgatcgttgc
     5941 caatggcgca ccttacggga gcgaatcctt gtttaacagc ttgcggctgg ccattgcgtt
     6001 acgagagcag gagaacaatc tggatctgcg tctgttcctg atgtctgatg cggtcacagc
     6061 cgggttgcgc gggcaaaaac caggggaagg ctacaacatt cagcaaatgc tggagatcct
     6121 taccgctcag aatgtaccgg tgaaattgtg caaaacctgt accgacgggc gcgggattag
     6181 tacacttcct ctgattgatg gggtggaaat cggtactctt gttgaactgg cgcaatggac
     6241 gctgtcagcc gataaagtgc tcacatttta atgtttgagg gggcgtctga cgccccacta
     6301 tgaggaaagt ttactcaggc gaatcccggc cgtaacacac catccggata attctccgcc
     6361 agcagttttt ttaccgatac cagcaattca ttcagcccat cgtcctgcat tcccagtttg
     6421 ataagctcct gttccagtga aaacaggtat tgtgcattgg ttcccagcgg accgctcgcc
     6481 gccgcaatca acggcgctat gacctgagcg cgagtgtcag attcatattc tggatgtcgc
     6541 gggtccataa taaacacaat ggcgtttact gtgcgtccat catcaagatc aagctgacac
     6601 caggttggca gataacagcc ggtaatcatc tcgcgcttcc acagcagggt tagttcctgc
     6661 tccagcgtct cttctggcag tcgataggcg acgcctgtgg tgcgtccgcc ctctttcagt
     6721 gcaagcattc gtcccggctg gtgcgcagtc ccgcgcccgg cggtcaggcg caggcaaaac
     6781 gcgcgatgcc atccaaccag tgtaccggtg cacgactcgg taaactccag tgccggattc
     6841 cacatcaacg atccataacc gaagatccac accggtccct catcaggtcg acaagccagc
     6901 gtcgccgcca gcgaagccgc ccgttgttct gctgaccata agagtgattc ttcaatggca
     6961 ccaaatgccg ttttacaatc ggcattcatc aagaaatcac gcgttatcac cttacacctc
     7021 cgccactgca tacttccctg cgacaacttt ttggtgtctt ccggacattt ttactgctca
     7081 ttttgaaacc actaacggga caatatgcaa tccacggacc acttgcaagg caacaccgca
     7141 cgaatagctt taactaagct accggtttta cgatttttta tgccatttat catcatcccc
     7201 tttggcatat tcatgcttca cagcagccca ggccacttta tgcgctgttt cttcgcgact
     7261 ggcgtcatca cgccgatctt ctttatcttt atattgatcc catgcgctgt tgaacgcttc
     7321 tttatagata tcctggacat gagacggaag cacgtgcttt acgctttccg gcagatcgct
     7381 tttcgtttta tacggcatcg ttgactcctt ttacaaagtt gaacattaag tgtggtcaac
     7441 aatatctact cttgccaaaa cataagaatt ttcatgtcag actcttctta agaagtgcta
     7501 aaacaaacat tgtctaaacg tattacttat ttttatgccc aaatgagtaa aaaatcgaca
     7561 gcggggtaaa aattcgtaaa atgttaccga tatccagaca ctttctgtta tttttcctac
     7621 ctatttttta cattttcgat aaattcatct gcaaagggat aaccataatg tcaaatgctc
     7681 aagaggcggt aaaaacccgc cacaaggaga cttcgcttat tttcccggtt ctggcgctgg
     7741 tagtgctgtt cctgtgggga agcagccaga cactaccagt ggtcattgcc atcaatcttc
     7801 ttgcgcttat tggtatttta agtagcgcct ttagtgttgt ccgtcatgcg gacgtattag
     7861 cccatcgcct gggagaacct tacggttcgc ttattcttag cctttcagtg gttattcttg
     7921 aagtcagttt gatttcagct ttaatggcaa ccggcgacgc cgcgccaacg ctaatgcgtg
     7981 atacgctcta ttcaatcatt atgattgtta caggtgggct ggttggcttt tcattattgt
     8041 tgggcggacg taagtttgcc acccaatata tgaatctgtt tggtatcaag cagtatttaa
     8101 ttgcgctgtt ccccctggcg ataatcgtac tggtatttcc aatggctctg cctgcggcga
     8161 atttttcaac aggtcaggcg ttactggtag cattaatttc tgcggcaatg tatggcgtat
     8221 ttttgctgat ccagaccaaa acacatcaaa gtttgtttgt ctacgagcac gaagatgaca
     8281 gtgatgatga cgacccgcac cacggtaaac cgtctgccca tagcagcctg tggcatgcca
     8341 tctggttgat tgtccatctg attgccgtta ttgcagtcac caaaatgaac gccagcccgc
     8401 tggagacatt gctcgacagc atgaatgcgc ctgtcgcctt tactggcttc ctggtggctc
     8461 tgttgattct gtcgccggaa ggtttaggtg cgttaaaagc agtgttgaat aaccaggtcc
     8521 agcgcgcgat gaatctgttc tttggttcag tgttggcaac catttcgctt accgtacctg
     8581 tcgtcacgct aattgccttt atgacgggta acgaattgca gtttgcactt ggtgcgccag
     8641 aaatggtggt gatggtggcc tctttagtac tgtgccatat ctccttctcc accggacgaa
     8701 ctaacgtgct caatggcgca gcgcatctgg cactgtttgc cgcctatttg atgacgatat
     8761 ttgcctgaca gttatatctc ctccgatgac ggaaatcggg ggagataata gaaattcctg
     8821 cttattatat ttcttcatac tagctggaca ggttgttaat cagaattgca agcctgtacc
     8881 acaacctgca cgaaatgact gcaacagctg ttgaaaaacc ggaatgtact ctttattgca
     8941 aacatttcag ggatgaagtg aagatgaatt gcgacgtagt atccacacca agccggcgtt
     9001 atccggtgag gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggaaaga
     9061 gaaaaccccc gcacgttgag aggtataacc tgacaacaga acgggggcta atcttgaacc
     9121 agaacaattc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct atatgacgct
     9181 cgcgcagttt gccatgactt tctggcacga cctggcagca ccgatccttg cgggaattat
     9241 taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa ggtgaaaacg ggcgtcaggc
     9301 tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg taacagcctg cggccttttt
     9361 tgtatccggc acccatgaaa aaagtcttaa cgcagaacgc taagacttta tttttcaggc
     9421 aaaaaagatt acttgctggt atccagttct tcgaaacctt tcaccagatc atcaatcgct
     9481 ttcatttgct tgaggaacgg ttccagttta gccagcggca gcgcggatgg accatcacat
     9541 ttcgcatgtt ccggatccgg atgcgcttca ataaacagcc ccgccagacc taccgccata
     9601 ccggctcttg ccagctcagc cacctgagca cggcgaccgc cggaagctgc gccaaatgga
     9661 tcgcgacatt gcagtgcgtg ggtaacgtcg aaaatcaccg gcaaattacc agacactttc
     9721 ttcatgatgc tgaagcccag catatcaaca accaagttgt catagccgaa gttagcaccg
     9781 cgatcgcaaa gaatcacttt ttcgttgccg ccttctttga atttatcaac gatattaccc
     9841 atctgtcccg ggctgacaaa ctgcggtttc ttgacgttaa ttaccgcacc ggttttcgcc
     9901 atggcttcaa ccaggtcagt ctggcgagca agaaacgccg gcaactgaat cacatctaca
     9961 acatcagcaa cgggctgtgc ctggcttggt tcgtgaacgt cggtgataat tttcacgcca
    10021 aaagtctgct tcaactcctg gaagattttc atcccttctt ccaggcccgg tccacgataa
    10081 gagtggatgg aggagcggtt ggctttgtca aaagaggctt tgaacacgta agggataccc
    10141 agtttctggg tcacggttac gtagtgctcg caaatgcgca tcgccagatc gcgagattcc
    10201 aacacgttca taccgccaaa cagtacgaac ggcaggtcat ttgctacgtt gatgtcgcca
    10261 atgctaacca ctttttgttt cataggatcg ccttaatctt gagtaaaatg tcgattaatt
    10321 aatgcagcac aatatgttta tgcgcgatgt tatttatttg cgcacggatc atttcgctga
    10381 tcgggtcttc cggacactgt tcaacgaaat aacttaaatc gttcaacgca acgtgttcgc
    10441 aatccagttg cgcataaatc aacccgcgat cgcgaatttc atagggatct tccgggttga
    10501 attgtaataa agcttcgctg gtgcgtaacg ccagctccat ctgattttct tccatcaacg
    10561 aagctttgag tgtatccagc aatttgcgga ttacctcaat gttatcagct tcatcaaggt
    10621 cttcataaaa cagttccgcc gacgggctga tatttccctt taaccataca tccagcatat
    10681 gttcgcttaa cgattcaccg ttaaaaggat taatcagcca aatttcgcca tccggacatt
    10741 caatgcgcaa tatcagctgc gtagggaaaa tcaccggcag cagcggcaaa tcgagacgat
    10801 tcgcgaccca taataaaacc gcacctaatg ataccgcact gccctgtcga ttctttaaca
    10861 cctggtccag ccataatgca tcggaaagac gataaacgcc gcgtgaggct ttaaaccccc
    10921 attcgccgta aaacagcgcg attaattttt ctagctgctc ttctaatggc agaagctggc
    10981 taatttcttc cttcgccaga ctaacgagac gctccagttc gtcgtaaaca tcttgcgagg
    11041 gaaaatcgcg gcggattgct tcgcaagcca ggatcatgcc ttcgcacaat ggcgctttat
    11101 taaattcgaa atcagctaac gatctcatga cttaccccag taacggtact tttgtggtgg
    11161 cgagtttaat gatgatgtac agcaccacca acgccagcgg gaaggcaata atgcgcgcct
    11221 gctgactgtg catacgacga taatcgagtg caataaaacc caaaacaata taaatgataa
    11281 ctccaaacag cttttcagtc agccactgtg cctgcccgga aaatggctgg atgtgcgctt
    11341 tagccatcaa cgcaatgccg cttaacaata acagcgtatc gacaaccggc ggcacaatgc
    11401 gcgtccagcg agcaaatgcc tgtggatgct tctgataacg tagccagaaa cgtaaggtca
    11461 atagcccaac agaaagcgcg atactaatga gatgaacgct aagcaacgta gaaaaacttg
    11521 tcattgataa tagcggccga gcgttacgcg ctcgttatcc ccatagtcac ggcaggtttc
    11581 gacgtcatga taccccgcga agataaatgc ctgtcgcacc gcttcaccct gctgccagcc
    11641 atgctccaga agcagaaagc cgccggatac cagcgcgtta cgcgactgtt cgatgatatg
    11701 cacgatgtct gccattccac tgtctgccgc aaccagcgca gtgagcggct caaagcggac
    11761 atcgccttgt tgaagatgtg ggtcctgctc gtcaatatac ggcggattgc tgacaatcat
    11821 cgcaaactgc tgcccggcta gcgcgctaaa ccagtcgctt tgcagaatgt ggatattttt
    11881 gatagccaga tgctgggcat tacgttgcgc cagagagaca gcatctggca tacgatctac
    11941 agcggtaatt tcgcagtccg gacgctcgct ggccagcgcc agcgcaatcg ccccggtacc
    12001 cgtcccgaga tcgagaatac ggcaaggttg ttcaggcaac cgcgccagtg cctgctccac
    12061 cagacactcc gtatccgggc gcggaattaa ggtcgctggc gaaacaaata acggcaacga
    12121 ccagaattct cgcaccccgg ttaaatgagc aatgggttca ccatcgcgac gacgcgtcag
    12181 tagcgcatca agttgctgac attgttcgtc agtcagctgc gtttcaccaa aggcgagaat
    12241 aaaagtacgc cctctgccgg taacatgctc cagcaggatt tcagcatcac gccgcgggct
    12301 ttcgctcgcc tgaagttggc ttattgcttc acgtaaccag tgttgatatt ccattattcc
    12361 tgctcggaca acgccgccag ttggtcggcc tgatgttcct ggataatcgg ttcaatcagc
    12421 atatccagct taccttccat cacttcatcc aggcggtaga gcgtcaggtt gatgcggtga
    12481 tcggtaacgc gcccctgtgg gaagttgtaa gtacggttac ggtcgctgcg atcgccactc
    12541 cccagcaggt tacgacgggt agacgcttcg gcctgttgcc gttttgccat ttcagcagcg
    12601 tggatgcgag caccgagtac agaaagtgct ttagctttgt ttttatgttg tgaacgttcg
    12661 tcctgacatt caacaacaat cccggtcggc aagtgagtaa tacgaattgc cgaatcggtg
    12721 gtgttaacgt gctgaccacc cgcccctgac gagcggaaag tatctatgcg taaatctgct
    12781 gggttgatgt ccggcagttc tgcgtcaggc agttctggca ttaccgcaac ggtacaggca
    12841 gaagtatgaa tacgaccctg cgattccgta gcaggaacac gctgcacgcg atgaccgcca
    12901 gattcgaatt tcaaacgacc atatacaccg tcaccgctaa ttttggcgat gatctcttta
    12961 taaccaccat gttcaccctc gctggcgctc atgatttcta cacgccagcg gcgggcttcg
    13021 gcgtaacggc tgtacatacg gaacagatcg ccagcgaaca gcgccgcttc gtcgccgccg
    13081 gttccggcac ggacttcgag gaaggcgtta cgttcgtcat caggatcttt tggtagtaat
    13141 agaacctgta attgctgttc cagttgctcg cttttttctt tagcttcgcg cagttcatcc
    13201 tgcgccatct cacgcatttc aggatcgtcg agcatcatct gtgcggtttc gatatcttcc
    13261 tgaacctgtt gccagtcggt aaaacagcgc gaaacatcac ttaactgcgc atattcacga
    13321 gataatgcgc gaaaacgttc ctggtcagcg atggtttgcg catcacccag cagcgcctga
    13381 acttcttcat ggcgttcatg cagggcttcc agtttggcaa cgatagaaga cttcataggc
    13441 gtaaatgcac cctgtaaaaa aagaaaatga tgtactgcta ctccagcccg aggctgtcgc
    13501 gcagaatatt caggcgttcg ttatccccgt cacgggcagc ctgttgaagt gatttcgttg
    13561 gcgcatggat caggcggtta gtcagtttcc atgccagatc ttgcattatg gcttgcgcgt
    13621 cgccgccctg ctcaagagcg gctaatgctt tggcggttaa ctcatcgcga acttgttcag
    13681 cctggctgcg ataatcgcga atggtttcgc tggcgctttg tgcgcgtagc cacgccataa
    13741 attcgctagt ttcctgagca acgatagttt ccgcctcaac tgcggcggct ttacgttgcg
    13801 ccaggttgtg cgaaataatg ctttgcagat cgtcaacgct ataaagataa gcattcgcca
    13861 gtttaccaac ttccggctca acatcgcgcg gaacggcaat atccaccagc agcattggtt
    13921 gattgcgacg gctttttaat gcgcgctcca ccatgccttt accgataatc ggcaacgggc
    13981 tggcggtgga actgatgatg atatcggctt cgcgcagacg ttcgtcgata tcgctcaggg
    14041 caatcacttc cgcgccaacc tcatctgcca gaatttgggc acgttcgcga gtgcgattgg
    14101 cgataatcat cttctgtact ttgtgttcgc gcagatgacg cgccaccagc tcgatggttt
    14161 cgcccgcgcc aaccagcagt accgtcaccg tagagagcga ttcaaagatc tgccgcgcca
    14221 gcgtacaagc cgcaaaagcg acagacacag cactggcacc gatatctgtt tcagtgcgaa
    14281 cgcgtttcgc cacagagaaa gatttctgaa acatgcgttc cagttcgctg gccttcatat
    14341 gacctttttg cgaatcggca aacgcttttt tcacctgacc gaagatctgc ggctcgccaa
    14401 ggacgagaga atcaagccca ctggcaacgc gcattaaatg actgaccgca tcgttatcct
    14461 gatgccagta gaggctatta cgcagatcgt cttcgttgag attatggtaa tcacacaacc
    14521 agcgaatgag cgcctcttgc aggtcatccc gttcttcaac gctgagataa agttctgtgc
    14581 ggttacacgt cgacagcacc acaccgccct gcaccatcgg ctgcgcgagc agactgtcaa
    14641 gcgcctgatc gagcttatcc ggcgaaaacg atacacgttc tcgcagcgat acaggtgccg
    14701 ttttatggtt gataccgagt gctaaaaggg tcatgtctgc gggaaataat accaacgttg
    14761 atagggttag tctgcttgca tcatacagga tgcgtaagat caataaaaga gagcgccccc
    14821 ttttggagta attgccgaaa gccgttagat tctggcaatt aagacaacgt gaacatagac
    14881 gatggctaac ggtaacgcta gcattaaggg ttataactgc aacgtatctc aaggacttgt
    14941 cattattatg cccctgcccg attttcgtct tatccgcctg ctaccgctgg ctgctcttgt
    15001 gctcactgcc tgttccgtta ccacgcccaa aggtcctggc aaaagcccgg attcgccaca
    15061 atggcgtcag catcagcaag acgtgcgcaa tcttaatcag tatcaaactc gcggcgcgtt
    15121 cgcttatatt tctgaccaac aaaaagtgta cgcccgtttc ttctggcaac aaactggcca
    15181 ggatcgctac cgtctgctgc tgactaaccc attgggcagc acggaactgg agctgaatgc
    15241 tcaaccgggt aacgtgcagt tagtcgacaa taaaggtcag cgttatacct ccgatgacgc
    15301 cgaagagatg attggcaaat tgaccggaat gccaattccg ctcaacagct tgcgccagtg
    15361 gattttaggt ttaccgggtg atgcgactga ctacaaactt gacgaccagt accgcctgag
    15421 tgaaatcacc tacagccaga atggcaaaaa ctggaaagtt gtttacggtg gttatgacac
    15481 aaaaactcaa cctgcgatgc cagccaatat ggaactcacc gacggtggtc aacgcatcaa
    15541 gttaaaaatg gataactgga tagtgaaata atgcggacac agtggccctc tccggcaaaa
    15601 cttaatctgt ttttatacat taccggtcag cgtgcggatg gttaccacac gctgcaaacg
    15661 ctgtttcagt ttcttgatta cggcgacacc atcagcattg agcttcgtga cgatggggat
    15721 attcgtctgt taacgcccgt tgaaggcgtg gaacatgaag ataacctgat cgttcgcgca
    15781 gcgcggttgt tgatgaaaac tgcggcagac agcgggcgtc tttcgacggg aagtggtgcg
    15841 aatatcagca ttgacaagcg tttgccgatg ggcggcggtc tcggcggtgg ttcatccaat
    15901 gccgcgacgg ttctggtggc gttaaatcat ctctggcaat gcggactaag catggatgag
    15961 ctggcggaaa tggggctgac gctgggcgca gatgttcctg tctttgttcg tggtcatgcc
    16021 gcgtttgccg aaggcgttgg tgaaatacta acgccggtgg atccgccaga gaagtggtat
    16081 ctggtggcgc accctggtgt aagtattccg acaccggtga tttttaaaga tcctgaactc
    16141 ccgcgcaata cgccaaaaag gtcaatagaa acgttgctaa aatgtgaatt cagcaatgat
    16201 tgcgaggtta tcgcaagaaa acgttttcgc gaggttgatg cggtgctttc ctggctgtta
    16261 gaatacgccc cgtcgcgcct gactgggaca ggggcctgtg tctttgctga atttgataca
    16321 gagtctgaag cccgccaggt gctagagcaa gccccggaat ggctcaatgg ctttgtggcg
    16381 aaaggcgtta atctttcccc attgcacaga gccatgcttt aagccgggca agctgagttt
    16441 cggtgacaac gtcaccttgt tccagacgtt gcatcgcgct ctttaataca ccgcctggaa
    16501 agaatcatgc ctggcccgca cagttttcgg cagattcttt ccaccaatgg acgcatgcct
    16561 gaggttcttc tcgtgcctga tatgaagctt tttgctggta acgccacccc ggaactagca
    16621 caacgtattg ccaaccgcct gtacacttca ctcggcgacg ccgctgtagg tcgctttagc
    16681 gacggcgaag tcagtgtaca aattaatgaa aatgtacgcg gtggtgatat tttcatcatc
    16741 cagtccactt gtgcccctac taacgacaac ctgatggaat tagtcgttat ggttgatgcc
    16801 ctgcgtcgtg cttccgcagg tcgtatcacc gctgttatcc cctactttgg ctatgcgcgc
    16861 caggaccgtc gcgtccgttc cgctcgtgta ccaatcactg cgaaagtggt tgcagacttc
    16921 ctctccagcg tcggtgttga ccgtgtgctg acagtggatc tgcacgctga acagattcag
    16981 ggtttcttcg acgttccggt tgataacgta tttggtagcc cgatcctgct ggaagacatg
    17041 ctgcagctga atctggataa cccaattgtg gtttctccgg acatcggcgg cgttgtgcgt
    17101 gcccgcgcta tcgctaagct gctgaacgat accgatatgg caatcatcga caaacgtcgt
    17161 ccgcgtgcga acgtttccca ggtgatgcat atcatcggtg acgttgcagg tcgtgactgc
    17221 gtactggtcg atgatatgat cgacactggc ggtacgctgt gtaaagctgc tgaagctctg
    17281 aaagaacgtg gtgctaaacg tgtatttgcg tacgcgactc acccgatctt ctctggcaac
    17341 gcggcgaaca acctgcgtaa ctctgtaatt gatgaagtcg ttgtctgcga taccattccg
    17401 ctgagcgatg aaatcaaatc actgccgaac gtgcgtactc tgaccctgtc aggtatgctg
    17461 gccgaagcga ttcgtcgtat cagcaacgaa gaatcgatct ctgccatgtt cgaacactaa
    17521 tcgaacccgg ctcaaagacc cgctgcggcg ggtttttttg tctgtaatat ccatttgtat
    17581 gacctatgcc tccttcacct gccatttagt tgacagatga tgcgctcacg gatgaaacat
    17641 tattgtgaac aaaatatttt cctcacatgt gatgcctttc cgcgctctga tcgacgcttg
    17701 ctggaaagaa aaatatactg ccgcacggtt tacccgtgac ctgattgccg ggataaccgt
    17761 cgggattatt gctatcccgc tggcgatggc gttggctatt ggtagtggtg tggcccccca
    17821 gtacggttta tataccgcag ctgttgcggg gattgtcatt gctctgacgg gggggtcacg
    17881 ctttagcgtt tccggtccga ctgcggcatt tgtggtaatt ctctatcccg tttcgcaaca
    17941 gtttggactg gcaggactgc tggttgcgac cttgctgtcg gggatctttt tgattctgat
    18001 ggggctggca cgctttgggc gcctgattga gtatattccg gtttccgtca ccttaggttt
    18061 cacctcgggt atcgggatca ccatcggtac catgcagatt aaagattttc tcggtctgca
    18121 aatggcccat gtcccggaac attatctaca aaaagtcggc gcattattta tggcgctgcc
    18181 gaccattaat gtgggtgatg cagccattgg cattgtgacg cttggcattc tggttttctg
    18241 gccgcgtctg ggcatacgtt tacccggcca ccttccggcc ttgctggctg gttgcgcggt
    18301 gatggggatt gttaacctgc tcggcggaca tgttgctacc atcggttcgc aattccacta
    18361 cgtcctggcc gatggttctc agggtaacgg tattccgcaa ctgctgccgc aactggtgct
    18421 gccgtgggat ctgcctaatt cagaattcac gctaacctgg gattctattc gcaccctgct
    18481 gcctgcggca ttctcaatgg caatgctcgg cgcaatcgaa tctctgctct gcgccgtggt
    18541 gctggatggt atgaccggga cgaaacacaa ggcgaacagc gaactggttg gacagggact
    18601 ggggaatatt atcgctccgt tctttggtgg tattaccgct acagctgcca tcgcgcgttc
    18661 tgccgctaac gtccgtgccg gggcaacttc ccctatctcg gcggtgatcc actctattct
    18721 ggttattctt gccctgctgg tactggcacc gctgctctcc tggctgccgc tttccgccat
    18781 ggcagccctg ctgttgatgg tggcgtggaa catgagtgaa gcgcacaaag tggtcgactt
    18841 gctgcgtcat gcaccgaaag atgacatcat cgtcatgctg ctgtgcatgt cgctgaccgt
    18901 gctgttcgat atggttattg ccatcagcgt ggggatcgtg ctggcatcgc tgctgtttat
    18961 gcgtcgtatc gcacgtatga ctcgcctggc accggtagtc gtagacgttc cagacgatgt
    19021 cctggttctg cgcgttattg gcccgctgtt ttttgctgcc gcagaaggct tattcacgga
    19081 cctggagtca cgtcttgaag gcaaacggat tgtgattctg aagtgggatg ccgttccggt
    19141 acttgatgct ggtggtcttg atgcgttcca gcgttttgtg aagcgtctgc ccgaaggatg
    19201 tgaactgcgc gtgtgcaacc tggaattcca gccactgcgc actatggctc gcgcaggcat
    19261 tcaaccgatc ccgggacgcc tcgcattctt cccgaatcgt cgcgcggcga tggcggattt
    19321 ataagtacga gattgaccag tcagcgcaaa ctgactggtc agcaaactgc attatttgtt
    19381 agctatgacg gcgattatcg ctacggcggc aacgtttgtc atagtggcga acccaccagt
    19441 aacggtcgca cacctgttca tgcccgccaa cacgggcacc tgccagccag agaatcgcac
    19501 cgacgaaaat acttagcact gcaccatgtg cgaaatactg gggcatatta aactgtggta
    19561 actggttgag gattgaatac cccacgccga ccaccattac caccagaccc aaccccatga
    19621 gcacgttacc gagtaacgaa gcgtttttgc gtttcatatg tcacctccgg aactttctgg
    19681 gttgtaacag ggaatacccc tcttccttat gtgtaaagta tagacaacac cctgtaggtt
    19741 atgtgcgggt gtgatcacaa ttacacccct tatttcaaca aaactttaca aataaacgcc
    19801 tgaacacctt gactttctaa tagtccactc aggcaataat tgcgttgtct gcatttgata
    19861 cgcagttttt tgtcaagcgg ggccgcaacc agtaaactac gcgccagtta tgtacacact
    19921 caggacaaaa aaacgtgacg attaaattga ttgtcggcct ggcgaacccc ggtgctgaat
    19981 acgccgcaac gcgacataat gctggtgcct ggttcgttga cttactggca gagcgattgc
    20041 gcgctccgct gcgcgaagag gctaaattct ttggttatac ttcgcgagtc acccttggag
    20101 gcgaagatgt ccgcctgtta gtcccgacta catttatgaa tctcagcggc aaagccgttg
    20161 cggcgatggc cagttttttc cgcattaatc cggacgaaat tctggtggcc cacgacgaac
    20221 tggatctgcc tcctggcgtc gccaaattta agttgggtgg cggtcatggt ggtcacaatg
    20281 gactgaaaga catcatcagt aaattgggta ataaccctaa ctttcaccgt ttacgcatcg
    20341 gaatcggtca tccgggcgat aaaaataaag ttgtcggttt tgtgttaggc aaaccgcctg
    20401 ttagtgaaca gaagttaatt gatgaagcca tcgacgaagc ggcgcgttgt actgaaatgt
    20461 ggtttacaga tggcttgacc aaagcaacga accgattgca cgcctttaaa gcgcaataag
    20521 tcgttgtctg cggcattttt gccaagtgcc gtgtataata ggcaaagtta tttccatttc
    20581 tgcaatctgt tagcaataac aggttgatta ttaagatatt aaggtgattt aaatcatggg
    20641 attcaaatgc ggtatcgtcg gtttgcccaa cgtcgggaaa tctaccctgt tcaacgcgct
    20701 gaccaaagcc ggtattgaag cggccaactt tccattctgc accatcgagc cgaacacggg
    20761 cgtcgtacca atgcctgacc ctcgcctgga tcaactggct gaaatcgtaa aaccgcagcg
    20821 tacacttccc acgaccatgg aatttgtcga tatcgccggt ctggtaaaag gcgcatcgaa
    20881 aggcgaaggt ctgggtaacc agttcctgac caacatccgt gaaaccgaag cgatcggtca
    20941 cgttgttcgc tgctttgaaa atgacaacat cattcacgtt tccggcaaag ttaacccggc
    21001 ggatgatatt gaagttatca acaccgaact ggcgctggcg gatctcgaca cctgtgaacg
    21061 tgcgattcat cgcgtacaga agaaagccaa aggtggcgat aaagacgcga aagccgagct
    21121 ggcggtactg gaaaaatgcc tgccccagct ggaaaacgca ggtatgctgc gtgcgctgga
    21181 tttaagcgct gaagagaaag cggctgttcg ttacctgagc ttcctgacgc taaaaccaac
    21241 aatgtacatc gccaacgtca acgaagacgg ttttgaaaac aacccgtatc ttgaccaggt
    21301 gcgtgaaatc gcagcgaaag aaggttctgt tgtagttccg gtttgtgctg ctgttgaagc
    21361 agacattgcc gaactggacg acgaagagcg tgacgagttt atgcaggagc ttgggctgga
    21421 agagccgggc ctgaaccgtg tgatccgtgc cggttataag ctgctgaacc tgcaaactta
    21481 cttcaccgct ggggtgaaag aagtgcgtgc atggaccatt ccggttggtg caaccgcgcc
    21541 gcaggcagcg ggcaaaatcc atactgattt tgaaaaaggc tttatccgtg cacaaaccat
    21601 ctcgtttgaa gatttcatca cttacaaagg tgaacaaggc gcgaaagaag caggcaaaat
    21661 gcgtgcagaa ggcaaagatt acatcgttaa agatggcgat gtgatgaact tcctgttcaa
    21721 cgtctaattc cgctaatact accctggcgg ttaataacga gccattatgt gagttgcacg
    21781 taatggcttt tttacgcctg ctacgcgtct tctttcaatt ctgagttacc aaaactcata
    21841 atcagactcc tgacaatatt aacaatgcag cttttatgtg ataacaatca cagtgcatta
    21901 accgttcaag agtttgtcat gaaaaaaaca tttaccttca gcctgttagc aattgtcttt
    21961 gcaagcctgt tgagtgcttg tgttccccac catcatcacc gccacagcga tggccctcgt
    22021 ggtcctgcgc cctcaggtaa gccaatgcca ccttcaaatg gtggaccaaa tcacggcagt
    22081 catggtcgct attaattagc aattcatagc gggaaagtac atactctccc gctatccacg
    22141 gcgcttaaac tgccctgcat caatgccatg ttgcttcatt ttccgccaca gcgtagtgcg
    22201 gccgatccca agtaaagccg acatttcctg aatgcgaccg cctgtgacct gggctgcgtt
    22261 aataattgcc tctttttcaa cttccgcaaa tgacagactg gtggaaaggc gggtggcgct
    22321 gacatcatct gtcgcctgct cggtaaacag atgttccggc aaatcactga cgcgaatgcg
    22381 cccattatca ctactcagag caagattctc gatgacgctg taaagttcaa aatcgttgcc
    22441 aggccatgca caggaaacca ggcgagcgag ggcatcgtca tcaattttca gccgcgtaga
    22501 gaagcgtttt tcaaggctgc gtaatttgtt attcaccagc gccggaatgc tgccacgccg
    22561 catacgcagt ggcgggatgg taatttcaaa tgcatgcagc gcgtaataca gctggcgact
    22621 aaaacgattt tgttccacca gcattgcgag gtccgcggtc gttgtggcaa tcactttgac
    22681 atcaatgggt attaaacgcc gcgcatccag tcgcgtgata accccctgct tgataacctg
    22741 aagcaaagca gactgtaact ccaccgccag atattcaatc ttttcaagaa acagcgtgcc
    22801 gccgtgcgcc agttccagcc gactcagacg gccattttca ttatccgtgc gatcgccacc
    22861 aataaattct tccgccagcg ccgcatcacc atataattca caattgacgg cgatataagg
    22921 ccccgctgca cgctcgcttt cattatgaat tgcctgactt agcagtgcct tgcccacgcc
    22981 ctcttctcca caaagcagga caggaaagct actgcgcgcc gcctggcgac caaaatgaat
    23041 caagcggcgg gtttgcggat cgtcctgtgg catatgagcg aaggtatggc tgacttttcc
    23101 taattgactg gtcattaact gccgcatctg ttccacagga tggagcaaca aaataaagct
    23161 ggttccctgc gtttctatta tcggtttaag ggttatcacc gcatcaataa actgatgctg
    23221 actttcaaag gtggcttcta cgtgtttgag cggatgtgcc tgttttattg cttgttgcaa
    23281 tacggcgggt aacgttaaga gttcagtgat tgcccttccc tgacttgccg tcgcgtcaag
    23341 gcgcaagact cgcgccgcct gggcgttgat aaattgcaaa ttaccctgct cgtcccagct
    23401 aatcacgcca tcatccatac tttctaacag ggcattaagt tgatttaaat gacggttagt
    23461 ttcagcgagc aaactgtccg tcagcagtaa atttccgacc tcgcgtgcga ttgccaacgt
    23521 caacggcaaa tcagctgcgg tagtttgctc aaccggacac gccagcgcta ttgttcccgt
    23581 caatcggccc ttgctgtcaa acaacggcgt tgcgcaaaag gcccagttcc agagtgcctg
    23641 tttgaaatgt tgatcggcca tcgttttcac ggcctgacca gagatagccg ctaacgatag
    23701 cgcacaagta ccaataattc cctcggcgca atacgtgccg tcattgaatc ccagtacact
    23761 tagctgctgc aaggtttgcg gatcgccatt acggctgaga atgcaggcgg tttcatcgag
    23821 gataagcaac gcacactctc gcggtgccat atattcccag gcatcttcca gcgcagcctg
    23881 cccgagcgtc agcatcgctt tcttacgccg ataaatagaa gcaaatgtca cgccctgggc
    23941 ctggtgtggt acgttccatg tctcccgttt catcagctta ttgcatctct cccaggaggt
    24001 tgcaattaag ggagatatgc cccgaccatc gttgttaaaa gcgccactca tatcccgcat
    24061 cccttatgac aaaaggtgtt ccggctcgca attttaagta ttaatacacg taaaagtttc
    24121 aatatggaac attcattaaa gcatatgttt cactatgaaa cctaatttgg gctaattttc
    24181 ttttcccttg cctgatgcac aatggattca tcggcagtca gtggtcgccg tgtcgttgaa
    24241 catcatccat gccctaccgt aattgctgga gcaaaataat gaaaaaattg atcaatgatg
    24301 tgcaagacgt actggacgaa caactggcgg gactagcgaa ggcgcatcca tcgctgacac
    24361 tgcatcagga tccggtgtat gtcacccgag ctgatgcccc cgttgcaggg aaagtcgccc
    24421 tgctgtcggg tggcggcagc ggacacgagc cgatgcactg tggctatatc ggtcagggga
    24481 tgctttctgg ggcctgtccg ggcgaaattt tcacctcacc gacgcccgat aaaatctttg
    24541 aatgcgccat gcaaattgat ggcggcgaag gtgtactgtt gattatcaaa aattacaccg
    24601 gcgatattct taacttcgaa acagcaaccg agttactgca cgatagcggc gtaaaagtga
    24661 ccactgtggt cattgatgac gacgttgctg taaaagacag tctttatacc gccgggcggc
    24721 gcggcgttgc caacaccgta ttaattgaaa aactcgtagg tgcagcggcg gagcgtggcg
    24781 actcactgga cgcctgtgcg gaactggggc gtaagttgaa taatcaaggc cattcaatag
    24841 gtatcgctct cggtgcctgt accgttcctg ccgcgggcaa accttctttt accctggcgg
    24901 ataatgagat ggagtttggc gtcggcattc atggtgagcc aggtattgac cgccgcccct
    24961 tctcttccct tgatcaaacc gtcgatgaaa tgttcgacac cctgctggaa aatggctcat
    25021 accatcgcac tttgcgtttc tgggattatc aacaaggcag ctggcatgaa gaaccacaaa
    25081 ccaaacaacc gctccagtct ggcgatcggg tgattgcgct ggttaacaat cttggcgcaa
    25141 ctccgctttc tgagctgtac ggcgtctata accgcctgac cacacgttgc cagcaagcgg
    25201 gattgactat cgaacgtaat ttaattggcg cgtactgcac ctcactggat atgaccggtt
    25261 tctcaatcac cttactgaaa gttgatgacg aaacgctggc actctgggac gccccggtcc
    25321 acaccccggc ccttaactgg ggtaaataag gagaaagcaa tgtcactgag cagaactcaa
    25381 attgttaact ggctcactcg ttgtggcgat attttcagca ccgagagcga gtatcttact
    25441 ggactggatc gcgagattgg cgacgctgac cacgggctaa atatgaatcg aggctttagc
    25501 aaagtggtgg aaaaactccc tgctatcgca gataaagata tcggtttcat tctcaagaat
    25561 accggcatga cactactttc cagcgtcgga ggtgccagtg gtccgctgtt cggtaccttc
    25621 tttatccgcg ccgcacagac aacccaggca cggcaaagcc tgacgctgga agagctttat
    25681 cagatgttcc gcgatggcgc ggacggcgta atcagtcgcg ggaaagccga acctggcgat
    25741 aaaaccatgt gtgatgtgtg ggtgccggtg gtggaatcgt tacgtcagtc ctgcgagcaa
    25801 aatctccctg ttccggcggc gctcgacgct gccagtagca tcgccgaatc cgctgcacaa
    25861 agtacgatta cgatgcaagc ccgcaaaggc cgcgccagtt atctcggtga acgcagtatt
    25921 ggtcaccagg atcccggcgc gacctcggtg atgtttatga tgcaaatgtt ggcgttagcc
    25981 gcaaaagagt aaggaattgg tgatggtaaa cctggtcata gtttcacata gcagccgact
    26041 gggagaaggt gtcggtgaat tagcccgtca gatgttaatg agtgatagtt gtaaaatcgc
    26101 cattgccgcg ggaattgacg atccacataa tcccattggt accgatgccg tcaaagtgat
    26161 ggaggccatc gaatctgttg ccgatgccga ccatgtgctg gtcatgatgg atatgggtag
    26221 cgcattattg agtgctgaaa ctgcgctgga attgctggct cccgagatcg ccgcaaaagt
    26281 acgtttgtgt gctgcgccgt tggtcgaagg tacactggca gcaacggtca gcgcggcctc
    26341 gggggcggat atcgacaaag ttatctttga cgccatgcat gcgctggaag ccaaacgtga
    26401 acaactgggt ttaccgtcct ccgacactga aatctctgac acatgtcctc cgtacgatga
    26461 agaagcccgt tctctgtcgg tggtcataaa aaaccgtaac ggcctgcatg tacgtccggc
    26521 ctcccggctg gtttatacct tatcgacatt taatgccgat atgttgctgg agaaaaacgg
    26581 caaatgcgtt acaccggaga gtattaacca gattgcgtta ctacaagttc gctataacga
    26641 tacgctgcgc ctgattgcga aaggaccaga agctgaagag gcactgatcg ctttccgtca
    26701 gctggctgaa gataactttg gtgaaacgga ggaagttgct ccacctactc tgcgtcccgt
    26761 tccgcctgtt tcgggtaaag ccttttatta tcaacctgtt ttatgtacgg tacaggcaaa
    26821 atcaaccctg accgtggaag aagaacaaga acgattacgc caagccattg acttcacgtt
    26881 attagatctg atgacgttaa cagcgaaagc agaaaccagc gggcttgacg atattgccgc
    26941 aatcttttct ggtcaccata cactgttaga tgatccggaa ctgcaggcgg cggcaagcga
    27001 actccttcag catgaacatt gcacggcgga atatgcctgg cagcaagttc ttaaagaact
    27061 tagccagcaa taccaacagc tggatgatga atatctacag gctcgctata tcgatgtgga
    27121 cgatcttctg catcgcaccc tggtccacct gacccaaacg aaagaagaac tcccgcagtt
    27181 taactcgcca actattctac tggcggagaa catttatccc tccacagtac tgcaactgga
    27241 tccagcggtt gtaaaaggta tctgccttag cgccggaagt ccgctatccc acagtgccct
    27301 aatcgcccgt gaactgggaa ttggctggat ttgccagcag ggtgagaaac tgtatgcgat
    27361 acaaccagaa gaaacgctaa cgctggacgt taaaacgcaa cgtttcaacc gtcagggtta
    27421 aaccaccgtt cggatggcat cattctgatg tcatccggca atttactctg cactgatgtt
    27481 ttttaactac ctgttatatt tttcaccaaa ttatgatgat ttttcttcgc taatcgcgaa
    27541 cccaacgtcg ctggctgacg aataaacaga tgttatttta tcactctatt tcgccgttat
    27601 ttatcccttt aattcccttt tctaaaatgt ctgtcagttc gcagaatgtg atttcgatca
    27661 tgcagctagt gcgatcctga actaaggttt tctgatactt gaataccgtt ttattcggtt
    27721 tcgccaaagg agaatgactg atgaaatccc ccgcaccttc tcgcccgcaa aaaatggcgt
    27781 taattccagc ctgcatcttt ttgtgtttcg ctgcgctatc ggtgcaggca gaagaaacat
    27841 cagtaacacc acaaccgcct gatattttat tagggccgct gtttaatgat gtgcaaaacg
    27901 ccaaactttt tccggaccaa aaaatctttg ccgatgccgt gccgaacagc gatccgctga
    27961 tgatccttgc tgattatcgg atgcagcaac accagagcgg atttgatcta cgccatttcg
    28021 ttaacgtcaa tttcaccctg ccgaaagagg gcgagaaata tgttccgcca gaggggcaat
    28081 cactgcgcga acatattgac ggactttggc cggtattaac gcgttctacc gaaaacaccg
    28141 aaaaatggga ttctctatta ccgctgccaa aaccttatgt cgtgccaggc ggacgttttc
    28201 gcgaggtata ttactgggac agttacttca ccatgttagg acttgccgaa agcggtcact
    28261 gggataaagt cgcggatatg gtggccaatt ttgctcatga aatagacact tacggtcata
    28321 ttcccaacgg caaccgcagt tactatttaa gccgctcgca gccgcccttc tttgccctga
    28381 tggtagagtt actggcgcag catgaaggcg acgccgcgtt gaaacaatac ctgccgcaaa
    28441 tgcaaaaaga atatgcttac tggatggacg gtgttgaaaa cctgcaagcc ggacaacagg
    28501 aaaaacgcgt tgtaaaactt caggatggta cccttctcaa ccgctactgg gacgatcgcg
    28561 atacgccacg accagagtca tgggtggaag atattgccac tgccaaaagc aatccgaatc
    28621 gacctgccac tgaaatttac cgcgacctgc gctctgccgc tgcgtctggc tgggatttca
    28681 gctcgcgctg gatggacaat ccgcagcagt taaacacctt acgcaccacc agcatcgtac
    28741 cggtcgatct gaacagcttg atgttcaaaa tggaaaaaat cctcgcccgc gccagcaaag
    28801 ctatcggaga taacgcgatg gcaaaccagt acgaaacgct ggcaaatgcc cgtcaaaaag
    28861 ggatcgaaaa atacctgtgg aacgatcaac aaggctggta tgccgattac gacctgaaaa
    28921 gtcataaagt gcgcaatcag ttaaccgcgg ccgccctgtt cccgctgtac gtcaatgcgg
    28981 cagcgaaaga tcgcgccagc aaaatggcga cggcgacgaa aactcatctg ctgcaaccag
    29041 gcggcctgaa caccacgtcg gtgaaaagtg ggcaacaatg ggatgcgcca aacggctggg
    29101 caccgttgca gtgggtcgcg acagaaggat tacaaaacta cgggcaaaaa gaggtagcga
    29161 tggacattag ctggcacttc ctgaccaatg ttcagcacac ctatgaccgg gagaaaaagc
    29221 tggtggaaaa atatgatgtc agcaccaccg gaacaggggg cggcggtggc gaatatccat
    29281 tacaggatgg ctttggctgg accaatggcg tgacgctgaa aatgctggat ttgatctgcc
    29341 cgaaagagca accgtgtgac aatgttccgg cgacgcgtcc actcagtgaa tcaacaacac
    29401 agccactaaa acaaaaagag gcggaaccta cgccttaact ttactgtata tcgagcaatg
    29461 ccattacgtg cataaaattg gcattgctct ttcttcatca caaaatac
//
LOCUS       DADTFG010000050        29276 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.050, whole genome shotgun sequence.
ACCESSION   DADTFG010000050 DADTFG010000000
VERSION     DADTFG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.050"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            175..846
                     /locus_tag="J1138_002024"
     CDS             175..846
                     /locus_tag="J1138_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBA8453827.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            856..2052
                     /locus_tag="J1138_002025"
     CDS             856..2052
                     /locus_tag="J1138_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA8453828.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2030..2611
                     /locus_tag="J1138_002026"
     CDS             2030..2611
                     /locus_tag="J1138_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA8453829.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDESAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPDGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2613..3386
                     /gene="yaiO"
                     /locus_tag="J1138_002027"
     CDS             2613..3386
                     /gene="yaiO"
                     /locus_tag="J1138_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBA8453830.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3573..3848
                     /gene="frmR"
                     /locus_tag="J1138_002028"
     CDS             3573..3848
                     /gene="frmR"
                     /locus_tag="J1138_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBA8453831.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3883..4992
                     /locus_tag="J1138_002029"
     CDS             3883..4992
                     /locus_tag="J1138_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBA8453832.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5085..5918
                     /gene="fghA"
                     /locus_tag="J1138_002030"
     CDS             5085..5918
                     /gene="fghA"
                     /locus_tag="J1138_002030"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBA8453833.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYNLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6045..6584)
                     /locus_tag="J1138_002031"
     CDS             complement(6045..6584)
                     /locus_tag="J1138_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBA8453834.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6686..7897)
                     /gene="mhpT"
                     /locus_tag="J1138_002032"
     CDS             complement(6686..7897)
                     /gene="mhpT"
                     /locus_tag="J1138_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBA8453835.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8073..9086)
                     /gene="dmpG"
                     /locus_tag="J1138_002033"
     CDS             complement(8073..9086)
                     /gene="dmpG"
                     /locus_tag="J1138_002033"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBA8453836.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALNAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9083..10033)
                     /locus_tag="J1138_002034"
     CDS             complement(9083..10033)
                     /locus_tag="J1138_002034"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBA8453837.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLSAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10030..10839)
                     /gene="mhpD"
                     /locus_tag="J1138_002035"
     CDS             complement(10030..10839)
                     /gene="mhpD"
                     /locus_tag="J1138_002035"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBA8453838.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPTGSLS"
     gene            complement(10849..11715)
                     /locus_tag="J1138_002036"
     CDS             complement(10849..11715)
                     /locus_tag="J1138_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBA8453839.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVAKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11733..12677)
                     /gene="mhpB"
                     /locus_tag="J1138_002037"
     CDS             complement(11733..12677)
                     /gene="mhpB"
                     /locus_tag="J1138_002037"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBA8453840.1"
                     /translation="MHAYLHCLSHSPLVGYVDLAQEVLDEVNGVIASARERIVAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12679..14343)
                     /locus_tag="J1138_002038"
     CDS             complement(12679..14343)
                     /locus_tag="J1138_002038"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBA8453841.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRILLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGMLRDSVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMALTRPDADVSVEKVA"
     gene            14535..15368
                     /gene="mhpR"
                     /locus_tag="J1138_002039"
     CDS             14535..15368
                     /gene="mhpR"
                     /locus_tag="J1138_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HBA8453842.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDSGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLATRPGDDYQLAREPLKLEAILARTRKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIKEGVESQAILVAGRRSGMHL
                     R"
     gene            15445..16527
                     /locus_tag="J1138_002040"
     CDS             15445..16527
                     /locus_tag="J1138_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA8453843.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16650..19724
                     /locus_tag="J1138_002041"
     CDS             16650..19724
                     /locus_tag="J1138_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBA8453844.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWSFAWFPAPEAVPESWLERDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERITRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSGTDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPMRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVINVDVAVASDTPHPARVGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGHYHYQLVWCQK"
     gene            19776..21029
                     /gene="lacY"
                     /locus_tag="J1138_002042"
     CDS             19776..21029
                     /gene="lacY"
                     /locus_tag="J1138_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HBA8453845.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21095..21706
                     /gene="lacA"
                     /locus_tag="J1138_002043"
     CDS             21095..21706
                     /gene="lacA"
                     /locus_tag="J1138_002043"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HBA8453846.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIYIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDFKVESSV"
     gene            complement(21827..23110)
                     /locus_tag="J1138_002044"
     CDS             complement(21827..23110)
                     /locus_tag="J1138_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HBA8453847.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLHDGKISAIDTQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLKDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(23100..24359)
                     /gene="codB"
                     /locus_tag="J1138_002045"
     CDS             complement(23100..24359)
                     /gene="codB"
                     /locus_tag="J1138_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBA8453848.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(24590..26476)
                     /gene="prpE"
                     /locus_tag="J1138_002046"
     CDS             complement(24590..26476)
                     /gene="prpE"
                     /locus_tag="J1138_002046"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HBA8453849.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHLQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(26516..27967)
                     /gene="prpD"
                     /locus_tag="J1138_002047"
     CDS             complement(26516..27967)
                     /gene="prpD"
                     /locus_tag="J1138_002047"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HBA8453850.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(28001..29170)
                     /gene="prpC"
                     /locus_tag="J1138_002048"
     CDS             complement(28001..29170)
                     /gene="prpC"
                     /locus_tag="J1138_002048"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HBA8453851.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEEAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEIFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
BASE COUNT         6829 a         8020 c         7612 g         6815 t
ORIGIN      
        1 catatgaact acataaaaga ttacacactc ttatccccgt acctgacagc cccttgtttt
       61 tcagctatca gctattttta acacttaaaa attagcatct gtctgcgtca gaggttacgt
      121 ctctgggggc gtcgttgtgc ttgtagtata ataaataaaa tggagtgtca taaagtggat
      181 aaggttttag attcagcgat cctttcttcg gcaaataaaa gaaagggtat ccttgccatt
      241 ggcgcgcatc ctgacgatat agaattaggc tgtggcgcat cgctagctcg ccttgcgcaa
      301 aaaggaattt atattgctac cgtagtgatg actaccggta attctggcgt agatggaata
      361 atcgatcgcc acgaagaatc acgcaacgct ctaaagatat tagggtgcca ccaaactatt
      421 catcttaatt ttgctgacac ccgcgctcat ttacagctca atgatatgat ttctgccctg
      481 gaaaacatca taaaaaatca aattccctct gatgttgaaa ttatacgtgt atataccatg
      541 catgatgctg accgccatca ggatcatctt gcagtctatc aagcctcaat ggttgcctgt
      601 cgcgctattc cgcaaattct cggctatgaa acaccaagta cctggctttc atttatgcct
      661 caggtttttg aatccgtgaa agaagaatat ttttctctta aactgactgc gttaaaaaaa
      721 cataaaagtc agagccagcg agattatatg cgcccggagc gtctgcgtgc agttgctcaa
      781 tttcgcgggc aacaggtcaa tagcgatctg ggtgaaggtt ttgttattca taaaatgatt
      841 ctttgagcaa attgcatgaa aacctggatc tttatatgta tggccgtagc aatattgcta
      901 tggtttctga gtacgttaag gcgtaagccc agccaaaaga aaggctgtat tgacgccatt
      961 atacctgctt ataacgaagg cccgtgtctg gcgcagtcac tggataatct gctgcgtaac
     1021 ccttattttt gccgggtaat ttgcgttaac gacggctcca cggacaatac cgaagcggtc
     1081 atggcggaag tcaaacgcaa atggggcgac cgctttgttg ccgtcacgca aaaaaatacc
     1141 ggtaaaggtg gtgcgctgat gaatggcctc aattacgcca cctgcgatca ggttttttta
     1201 agtgatgccg acacctatgt tccgcccgat caagacggaa tgggctatat gctggcggaa
     1261 attgagcgtg gcgctgatgc cgtaggcggc attccctcta ctgcgttgaa aggcgcgggt
     1321 ctgttaccac acatccgcgc gaccgtaaag ttgccgatga ttgttatgaa gcgcacgcta
     1381 cagcagctcc tgggcggcgc accgtttatc atcagcggtg cctgcgggat gttccgtact
     1441 gatgtattgc gtaagttcgg tttctcggat cgtactaaag tcgaagacct tgatctcacc
     1501 tggacgttgg tggcaaacgg ctaccgtatt cggcaggcga atcgctgcat cgtataccca
     1561 caggaatgta acagtccgcg tgaggagtgg cgccgctggc ggcgttggat tgtgggctac
     1621 gcggtctgta tgcgcctgca taaaagactt ttatttagcc gcttcggtat cttcagtata
     1681 tttcctatgc tgttggttgt gctttatggc gttggtattt atctcactac ctggtttaat
     1741 gaattcatca ccaccgggcc acatggtgtg gtgttggcaa tgttcccgct tatctgggtc
     1801 ggcgtagttt gtgttattgg tgcatttagc gcctggtttc atcgttgctg gttgttggtg
     1861 cctttagcac cgctttccgt tgtgtatgta ttattagctt atgccatctg gattatttat
     1921 ggacttattg ccttttttac tggacgcgaa cctcagcgcg acaaacccac ccgctattcc
     1981 gcactggtgg aagcgtcaac cgcttattcc cacccttctg tcacaggaac tgaaaaacta
     2041 tctgaagctt aatgttaaag agaaaaatat tcagattgca gaccaggtga ttattgatga
     2101 aagtgccggt gaggttgtta tcggcgcgaa tacccgtatt tgccacggtg ccgttattca
     2161 gggtccggta gtgattggcg caaactgcct gataggtaat tatgcgttta ttcgtcctgg
     2221 cacaataatc agcaatggcg taaaaattgg ttttgccacc gaaattaaaa atgcggttat
     2281 tgaagcggaa gcaacgattg gtccgcaatg ctttattgcc gactcggtag ttgcaaacca
     2341 ggcatatttg ggcgcacaag tacgaaccag taatcatcgt ctggatgaac agcccgtgtc
     2401 tgttcgaact ccagatggaa ttatcgctac cggatgcgat aaattaggtt gttatatcgg
     2461 gcagcgttca cgccttggcg tacaagttat tattttgcct gggcgaatta tttccccgaa
     2521 cacgcaactt ggcccgcgcg tgattgtaga acgtaattta cctaccggaa cttattcact
     2581 ccgacaagaa cttatccgta caggagatta aaatgataaa acggacgtta ttagcggcgg
     2641 caatttttag cgcattaccc gtttatgccg ggttaacttc cattaccgcg ggctacgatt
     2701 ttaccgatta ttctggcgat catggcaacc gtaatttagc gtatgctgaa ctggtggcga
     2761 aagttgaaaa cgcaacgctg ctttttaatc tttcccaggg gcgtcgtgat tatgaaactg
     2821 aacatttcaa tgccactcgc ggccagggtg cagtctggta caaatggaat aactggctga
     2881 caacccgaac gggtattgcc tttgcggata atacgccggt ctttgcccgc caggattttc
     2941 gtcaggatat taacctggcc ctgttgccaa aaacgctttt cacgaccggt tatcgctaca
     3001 ctaaatatta cgatgatgtc gaagtcgatg cctggcaagg tggcatatca ctctatactg
     3061 gcccggtgat caccagctat cgctataccc attatgactc cagcgatgca ggtggtagtt
     3121 acagcaatat gatttccgtg cgtctgaatg acccgcgcgg cactggttac acgcaactat
     3181 ggctaagccg cggaacaggt gcttacacct acgactggac gccagaaaca cgctacggca
     3241 gcatgaagag catcagtttg caacgtattc aaccgctgac tgagcaactt aatctcgggt
     3301 tgacggcagg taaagtgtgg tacgacaccc caaccgatga ttataacggt ctgcaacttg
     3361 cagcccatct gacctggaaa ttctgattcc ttctgccgcc cgctatccgg ggcggccttc
     3421 cctgccgatt agcccccccc ctttcctctt tgttttccga ccacattcac cggataaatt
     3481 ttattctcca gtgttatata ctataggggg gtatgcattg acatatagca taccccccta
     3541 tagtatattg cgtgcagatg atgaggtgcg aaatgcccag tactccggaa gagaagaaaa
     3601 aggtccttac tcgagttcgt cgtattcggg ggcagattga tgctctggaa cggtcgctgg
     3661 agggtgatgc cgaatgccgt gccatactcc aacagatcgc tgccgttcgg ggcgcggcta
     3721 acgggctgat ggcagaagtg cttgaaagcc atatccggga aacgtttgac cgaaatgact
     3781 gctacagccg cgaagtcagc caatccgttg acgacactat tgaactggtt cgtgcctatc
     3841 ttaaatagct gaatctatta ccatattgag gaagagcgag agatgaaatc acgtgctgcc
     3901 gttgcatttg ctcccggtaa accgctggaa atcgttgaaa ttgacgttgc accaccgaaa
     3961 aaaggtgaag tgctgattaa agtcacccat accggtgttt gccataccga cgcatttacc
     4021 ctctccggcg atgacccgga aggtgtcttc ccggtggttc tcggtcacga aggggccggg
     4081 gttgtagttg aagtcggtga aggggtaacc agcgtcaaac ctggcgacca tgtgatcccg
     4141 ctttacaccg cagagtgcgg cgagtgtgag ttctgtcgtt ctggcaaaac taacctgtgt
     4201 gtcgcggttc gcgaaacgca gggcaaaggc ttgatgccag acggcaccac ccgtttttct
     4261 tacaacgggc agccgcttta tcactacatg ggatgctcaa cattcagtga atacaccgtg
     4321 gtagcggaag tgtctctggc caaaattaat ccagaagcga accatgaaca cgtctgcctg
     4381 ctgggctgtg gcgtgaccac cggtattggc gcggtacaca acacagctaa agtccagcca
     4441 ggtgattctg ttgccgtgtt tggtcttggc gcgattggtc tggcagtggt tcagggcgcg
     4501 cgtcaggcga aagcgggacg gattatcgct atcgatacca acccgaagaa attcgaactg
     4561 gcgcgtcgct tcggtgccac cgactgcatt aacccgaatg actacgacaa accaattaaa
     4621 gatgttctgc tggatatcaa caaatggggt atcgaccata cctttgaatg catcggtaac
     4681 gtcaacgtga tgcgtgcagc gctggaaagc gcgcaccgcg gctggggtca gtcggtgatc
     4741 atcggggtag caggatctgg tcaggaaatc tccacccgtc cattccagtt ggtcaccggt
     4801 cgcgtatgga aaggttccgc gtttggcggc gtgaaaggtc gttcccagtt accgggtatg
     4861 gttgaagatg cgatgaaagg tgatatcgat ctggaaccgt ttgtcacgca taccatgagc
     4921 ctggatgaaa ttaatgacgc cttcgacctg atgcatgaag gtaaatccat tcgaaccgta
     4981 attcgttact gatttcccgc tggtttaccc cgtccagttc agatggggtt cttaatactc
     5041 tccccgggca gccgtccggg ggattaaccg tgagataatg actgatggaa ctcattgaaa
     5101 aacatgccag ctttggcggc tggcaaaatg tgtatcggca ttattcccaa tcactgaaat
     5161 gtgaaatgaa tgtcggcgtc tatctcccac caaaagccga gaatgaaaaa ttgccggtgt
     5221 tgtactggct ttcaggcctg acctgcaacg agcagaattt cattactaaa tcggggatgc
     5281 agcgttacgc ggctgagcac aacattattg ttgttgcgcc ggacaccagt ccgcgaggca
     5341 gtcatgtcgc agatgctgac cgttacaatc tcgggcaagg tgccgggttt tacctgaacg
     5401 cgacgcaagc gccgtggaat gaacattaca aaatgtatga ctatatccgc aacgagctgc
     5461 cgaatttagt gatgcatcat tttccagcaa cggccagaaa gtctatctca ggtcattcta
     5521 tgggcgggct gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat gtcagcgtct
     5581 cggcgttttc gcccattgtc tccccatcgc aagtaccgtg gggacagcaa gcctttgccg
     5641 catatcttgg tgaaaataaa gatgcctggt tggattacga cccggtgagt cttatttcac
     5701 aaggtcaacg cgttgcggaa atcatggttg atcaggggtt gagtgatgat ttttacgcag
     5761 aacaactgcg gactccaaat cttgaaaaga tctgccagga gatgaatatc aagacgttaa
     5821 tccgttatca cgagggttat gatcacagct attattttgt ctccagtttt attggcgagc
     5881 atattgcgta ccacgccaat aaactgaata tgcgttgagc atccgcgcac gcatgccgga
     5941 tgcggcgtaa acgccttatc cggcctacat ttcacccgca ggccgtaggc atgataaaac
     6001 gcgcatagcg tcgcatcagg catctgcgca cgacttatct atgtttacca catcaaatca
     6061 tcaggcactt tgaagtcggc atacggatcg tcttcatcct gctcttcggc gctgagcgcg
     6121 ctgtgcaaca caatactgct ggcatcacgc tgagcaattt tatcggcaac actggcggga
     6181 ataatggcgt attcaccttc gctattgtta tcgaccaaca agcgggcaat cgccagacga
     6241 ccattaatca actgcgcctg tgtgagctta tcgacaaaaa tcttcttaat cagattgccg
     6301 tcagtgaagt taaaaccaat atcgccattg gcaatggtga ttcggttcat ttcaatgagc
     6361 tgcttcacct gggctttata ttctttcgcc aacgccgctt gtttttgctg ttcgctaagc
     6421 tgtttatcac gctcaagctg tgccttttta ttttcttcta ccgccgcccg agcttcacgc
     6481 gcctgaacgc gcgatttttt cgccgttctc tccaccttcg ccgctttttt gctggtgact
     6541 agtcccgctt tgagcaactg ctcttgtaag gtaagttttg ccatctttgt ttccaaaccc
     6601 gctgaataat ttttagcgag tatacctgta aacgccgtag gtgtattggg ggcaagtccc
     6661 tttcgcccct gcttaataag gcgcgtcagg catcggcaca cggctgcatt cttgatctcc
     6721 gactcatcaa aataaacact gccaggccag caacaagaat acccggtgca gaagcggcca
     6781 ttacgccgac cgtgccagtg cctaatgcca gcattttccc ggccagtaac ggaccgctca
     6841 tagcccccag acgccctacc gccacggctg ttcccacacc tgttgcgcgg atctgcgaac
     6901 tgtaaaacaa cggtgccagg gcatacaaaa cgctttgccc acctgtcgca aacaaccccg
     6961 cgacgaatcc cgccagcaac ataccgttaa atgacgacac cgttcccagc gccagcagcg
     7021 aagctaacat gccgctataa atcagtagcg acatggttac tggacgcagc ttatccatca
     7081 atgcgcccaa cattaacgtc ccgcttgccg cccccatttg tagggcaaac atcacccctg
     7141 ccgcctgcga tggctggaat ccttgctcca ccaaaagcag cggtagccag ttgatcaaca
     7201 tgtagaccac cagcagagtg aagaaataac acaaccacag caacagcgtc gcggtggccg
     7261 tttctggcgc aaataacgca cgcagtggtg gcgcagcctg tttttcacca gcgaacaccg
     7321 ccgactccgg cagccagcgc attaatagcg gcaccagaat caacggcacc acaccaccga
     7381 cccaaaacac cgtttgccat gctaagtttg cccccgcgaa acccagtgtc gccgccagcg
     7441 ccgcgccaat gggaacaccg caatacatca ggctcactgc cgtcccacga aaacgtggac
     7501 ccgcggcttc agacgtcagg gcgataagat tcggcaacgc cgcccccagc ccgacaccgg
     7561 tcatcagccg cgcaaagacc agtgagggga aatcccaggc aatcgccgtt gccagtgaga
     7621 acaaaccaaa cagcgcaact gagccaatca aaatgcgctt gcgaccataa cggtccgcca
     7681 gcattccgcc aaccaacgcg ccgggtagca aaccgagtat tccggcgctg aatatccagc
     7741 ccatttgcat tttatcgagt gcgaaagcct gggcgatgcc acccgccgca atgccagccg
     7801 cctgaagatc cagtccttcc atcagagcga ccaaaaaaca aagcccgatg gtcagcatca
     7861 ggcgggttga agatgatgaa ggggtacgag tcgacataag atgaacctcg ttgcgagtgc
     7921 aggtgaatcg cggctgatac attgtcagcc gcgtaatcag aaccatttaa tgactgccta
     7981 cgggcgaaat ataggccgga taaggcgttt acgccgcatc cggcagtcgt gctgggatgg
     8041 gcatacaaaa tgcacgttct cggcagtcat cattatttgt tgttgcgcag atccagcgcc
     8101 acgtcgacga tcatatcctc ctggccgcca accatccggc gtttgcccag ctcaacgaga
     8161 atatccaccg cgcttaagcc ataacgcgcc gccgccgttt cacagtgacg caggaagctc
     8221 gagtaaacac cagcgtatcc cagcgccagc gtttcgcgat cgactcgtac cggtcgatcc
     8281 tgcaacggac gcaccaggtc gtcagcggca tccattaacg catagagatc ggtcccatgc
     8341 tgccaaccca gtttatccgc ggcggcaata aatacttcca gcggcgcgtt acctgcgccc
     8401 gcgcccattc ccgcgaggct ggcgtcgatt cggtcgcagc cctcttccac cgccgcgata
     8461 gagttcgcca cgccaagact caggttatgg tgagcgtgca tgccggtttg cgtttctggt
     8521 ttcagcactg cgttcagggc gcggaaacgg tcacggatat cgctcatgtt catcgcaccg
     8581 ccagaatcca ccacataaat acaggtcgca ccgtagcctt ccatcagctt tgcctgcttt
     8641 gcgagattct ccggcgtggt catatggctc atcatcagaa aaccaacggt gtccattccg
     8701 agctcgcggg catactgaat atgctgggcg gaaacatcag cttcggtaca gtgcgttgcc
     8761 acacgaacca cccgcgcgcc agcctgccag gcatttttca gatcgtgaat agtgccgatt
     8821 cctggcagta acaacgtcgc gattttggcg tgcttcacca catccgccgc cgcttcaatc
     8881 cattcaaggt cgctatgtgc gccgaaacca tagttaaagc tggaaccctg caaaccgtcg
     8941 ccgtgggcca cttcaatcga atccacgcgg gcatcatcca gtgctttggc aatctggcgg
     9001 acattttcca gcgaatactg atgacgaatg gcgtgcatac cgtcacgcaa cgtgacgtcc
     9061 gaaatataaa gttttttacc gttcatgccg cttctcctgc cttgcgcgcc agtgactggg
     9121 ccattttttc cgctgtcgcc agcgcactgg aagtcataat gtcgaggttg cccgcatagg
     9181 cagacagata atgcgctgcg ccttcgactt ccagccagac cgccgttttc agcccggaga
     9241 actgccccac gcccggcaaa ttaaccggtt tatcctgcgg gataacttca aactgcacgc
     9301 gctgtttcag gcgatatccc ggtacgtaag cctgcaccgc ctcagccatt tcattgattg
     9361 aggcttcgat atcatcttgt gaagcttcgt cgctcaatac atacaccgtg tcacgcatca
     9421 tcaacggtgg ctctgctggg ttaagcacaa taatcgcctt cccttttgcc gcgccaccca
     9481 ccacttcaat ggcgcgcgaa gtggtttccg taaattcatc gatattggca cgcgtgccag
     9541 gtccggcaga tttactggca atagaagcga taatttcggc gtaatgaaca cgcgccacgc
     9601 gtgaaactgc cgccaccatt ggaatggtgg cctggccgcc gcaggtgacc atgttgacgt
     9661 tcagttgagc gacgttctcc tcgaggttaa cgaccggcac gcagtagggg ccgatggcag
     9721 caggcgtcag gtcaattaag cgaatatccg gtttcgcttc gcgcaaagct gcatcgtttt
     9781 tcacatgcgc acccgcgctg gtcgcatcaa atacaatgtc gatatcagca aattcaggca
     9841 tgttcatcag cccgatcacc ccttcatggg tggtggcgac gcccatacgt ctggcacgcg
     9901 ccagaccgtc ggactgagga tcaatgccga ccatcaccgc catctccaga tgctggccgt
     9961 gacgcaaaat tttaatcatc agatcggtgc caatgttgcc tgaaccgata atggcgactt
    10021 tacgcttact catgacagac ttcctgttgg ggctgcgctt gaaaatgtcg cagcaactga
    10081 acctatgcct tcaatatggg cttcaaaacg atcgcccgca ttcactgcca ccatcggacc
    10141 taatgccccg gtaagaatga tatctccggc gcgcagcggt tcacccaagc tggccatttt
    10201 gcgtgccagc cagacggcgg cattaagcgg atgtcccagg cattcgctgc cgcgcccgct
    10261 ggaaacctct tcgttattac gcgtcatctt catggcgcag tttttcaggt ctaaccccgc
    10321 cggacgttgc gccggaccgc cgatgacata caccccgcag gaagcgttat ctgccacggt
    10381 atcgacaaac tgaatcgacc agtcgcgaat gcggctcccc accacttcca gcgccggaag
    10441 tacccattca atggcgttat acaattcgtc gaaggtgata tcggttgcgg gtagatcgcg
    10501 gttcaacacc agtgcaatct ccgcttcaat gcggggttgc agaacacggg aaaaaggaat
    10561 ggtttcgtta tcgccataac acatgtcggc aaataatgtc ccgaaatccg gttgatcaac
    10621 gcccagttgt tgctgcactt tcggatgcgt cagccccact ttgcgcccca ccactcgccg
    10681 tccctgagcg acgtcgtgtt gcacattgat gtgctgaatg gcgtaggccg cttcagcgtt
    10741 atcgatacca atcagatcgc gcagcggcgc aatcgcttcg ccctgctctg cggcgcggcg
    10801 taaatccgcc gccagttgct caagagtatg cttcgtcatg accgttcctt aaggacgtgc
    10861 gaggaaattc agcaccagtt gattgaaagc gtcggcatgt tcccactgcg cccagtgacc
    10921 acagtcgcgg aagatatgca gttcagaacc ggcaatgccg gacagcagac gcagacccgc
    10981 atccatcggc acaaagcggt cgttgcgccc ccagacaatc agggtttgcg ctttgatttc
    11041 tgccagacgt gggccaaaat ccgggaactg tttcggatta gcttccaggc tcttaacgaa
    11101 gttttccagg tgatcgcggc gcgacagcat attattcagg cgcgcttcaa acagggcgtc
    11161 ggtcaaatcg ctggtatcaa aaacgaagat atccatcatc agcttcaggt tttcgatagt
    11221 cggctgacga taaagctgat tcagtcgctt aataccttcg gttggcatcg gcgtaaacaa
    11281 actcatgccg cccgtaccgc cgcccatcag caccagtttg gcgacgcgct ccggccagtt
    11341 aagagtgaac gccacagaac tgtggccgcc catcgagttg cccagcaggt ggattttggc
    11401 gatatccagt tgatccacca cgcttttcag gattcgcgca ttaagatccg atcgcgaacc
    11461 actattaacg atcgaatcgc tcttacccca acccggacaa tccagcagga tcacccgata
    11521 gcccgcctct accagcggat cgatattgcg gctgaagttc gcccagccag tagcacccgg
    11581 gccggaacca tgcagcagga caacagtttc gtcgccttgt ccgcagtcat taaaatggat
    11641 gcgcagcgtt ttacccgctt cttctacatt cagaaaacgg ctggtggcgg cttcggtttg
    11701 tggctgataa ctcatcatct tctcctgcat attcagttct ctgttctggc gcttaacgag
    11761 ccaaatccgg caatccactc cgggattggg cggtaataac gcccttcgct acgccagttg
    11821 ccaaacgcag aaatagcggc aaaagcggcg acccaggttt tgatttcatg tgtcgacttt
    11881 ccggcaatgg cggaaagctc ttcgttactg acggcatcca gttcctgtat gcgtccctgc
    11941 tccagcaaag tcatgaactg gttatcccaa atcgggttga gcggatgcag cgttctctga
    12001 tcctcaacaa acttctcagc ggcgctaatc acccgttgct gacgcaattc gcgctcactg
    12061 gcgggtaaat ctttcccgct ccccaacaga cggtcgcgca tatgggcatc ggctttcgcc
    12121 agttctggta ccggtggctg atgggaaagc ccaccggacc caaggaacag cacgcgttta
    12181 ttgagagtgc tggtgaaacg tccaatggct tcccccaaca tgcgggtacg ctggaaaccg
    12241 ggcagcggcg tggcgacacc gttgatgaac accggcagta ctggcacctt atccagcccg
    12301 ccgagcagga actccagcgg ctgggcgaac ccgtggtcca cctgcataca gtaagaaacg
    12361 gcaagatcga tcccgctctt catgacggca tgtgcacagg cctccgccag ctctacaggc
    12421 acgggcagct ctcctgccgc actaccgaaa tcaccaattg ccgtcgctcc aacgcctaaa
    12481 cagaacggtg gcatcacgtc atagaaaaag ccgttgtagt gatctggcgc aaacagcacc
    12541 accagttcag gggagaaagc cacaatacgc tcgcgggcgc tggcaatcac gccattgacc
    12601 tcatcgagta cctcttgtgc caggtcgaca taccccacca gcggcgagtg ggaaagacag
    12661 tgaagataag cgtgcatatc aggctacctt ttcgacagaa acgtcggcat cagggcgggt
    12721 cagcgccatc accgacgcca gtttattcag ggtcttgccc agggtttgcg gaatggcggt
    12781 ggcggcaaca aagcgatccg ggcgcatcac caccagcgaa gcattgtgtt gcgcgaacca
    12841 gctacgcagg cgaccttgcg tatcccccac gcgtagtacg ccgtcgtggt tatcctgtgc
    12901 ggtatgaatt tgcacttccg gcaccacctg aatgaagcgt gtgcccaacg cgcgccactg
    12961 ctggatttgc tcgtcgctca tcccccacag tggattgcat ccccagccaa ttaccgcgaa
    13021 gttcgcgccg atcgcgttat cgagtagcgt cacgtcgccg ttttccagcg tgactttcgg
    13081 ctgaataaac atcttgccga ccggagagtg cttcgcttcg ccctcacgca ccagcgcacc
    13141 gccgtaatat tgcggcatcg gcttgaagcg catttcgagg aagtagcgtt ttactggcgg
    13201 cagataattc agcagccagg aaacgctgtc acgtaacatc ccctgccagc gtttcggcgg
    13261 agccagcaca ttgcccgccg tcacggacag gtcaatcatc gctttggcgt gatcgcgacg
    13321 ttcttgttga taggtatcga gcagcgcatc gcgggctttc ccctggataa ccaacgccag
    13381 tttccatgcg aggttaaagg cgtcgcgcat accactgtta tagccctgcc cctgccacac
    13441 cggcatgatg tgcgcggcat cgcccgccag cagtatgcga tcaatacgaa aacgttgcgc
    13501 cagtcgcgcg ttgtgggtgt agacacgctg gcgaatcaat tcaacattgt ccggattagg
    13561 cagcactttg cttaacagct tgcgcatatt ttgcggctca cgcagctgtt cttcggtttc
    13621 tcccggcatc accataaatt caaagcgacg taccgcatga ggcagcgcgg cagaaacata
    13681 cgggcgcacc ggatcacaac acaaatagat atgcggcgta cttaacggat cgttggcgat
    13741 atctaccaca atccactgat ttggcgcagt tttaccttca aacggcacat tcagggtgcg
    13801 acggacaaaa cttgccccac catcacaggc caccagccac tgggctttga ccgtttcccg
    13861 ctgcccttct gccgttttca ggtgcaaggt cacttcgtca tcttgctgac tgaaggcctc
    13921 cagctcgcgg gaaaacaggc agcgcacatt cggaaaacgc gacaatcctt ccagcatcac
    13981 cgcatcgacc tgaggctgaa taaaggcgtt acggcgcggc cagccaaatt catcggtcat
    14041 tggctgaata tcagcaaaac agcgaccttt tggggtgaga aaacgcatcg cgtgccacgg
    14101 cgtagtgtgc ggcagaacat tatcgaccag gccaaccgac tgcatggtgc gcagcgcctc
    14161 gtcatcaata ccaatcgcac gcgggtagtc gatcaactta tcgagtttct ccaccaccag
    14221 cacgtcaatg cccatctggc cgagatagtt cgccatcatc agcccgaccg gaccggcacc
    14281 agcgatcgcc acctgaacgc tatggttaac agcaggctgg atgtcagggt gttgtattgc
    14341 catttcagta cctcacgact cggacaaaat gccgttgcgc gcacagtaca gcgcaactta
    14401 ttttgttaaa aacatgtaaa tgattttttt attgtgcgct cagtatagga agggtgtttt
    14461 cggctacaat caaaacatgc ccgaatgtgc accaggtgca caacgttgtt ttaactatat
    14521 aaatatcaat taatatgcag aacaatgagc agacggaata caaaaccgta cgcggcttaa
    14581 cgcgcggtct aatgttatta aatatgttaa ataaacttga cagcggtgcc agcgtcgggc
    14641 tgttggcgga gctcagcggc ctgcatcgca ccactgtgcg gcgactgctg gagacgctgc
    14701 aggaagaggg atatgtccgg cgtagtccct ccgatgatag ttttcgtctg accatcaaag
    14761 tgcggcaatt aagcgaagga tttcgtgacg aacagtggat ttctgcactg gcggccccgc
    14821 tgctgggcga tctgttgcgc gaagtagttt ggccaacaga tgtgtccacc ctggatgttg
    14881 atgcaatggt ggtacgcgaa accacccacc gtttcagccg cttatccttt caccgggcaa
    14941 tggtcgggcg acgtttaccg ctcctgaaaa ccgcctcggg cctgacctgg ctggcctttt
    15001 gcccggaaca agaacgcaag gaattaatcg aaatgttagc cacccgtcct ggtgatgact
    15061 atcaactggc acgggaaccg ttaaaactgg aagccattct ggcgcggacg cgcaaagagg
    15121 ggtacggaca gaactaccgc ggctgggatc aagaagaaaa gatcgcctcc attgccgtac
    15181 cgctgcgcag tgaacaacgg gtgattggct gtctgaatct ggtgtatatg gcgagcgcaa
    15241 tgaccattga acaggcagcg gaaaagcatc ttccggcgct acaacgggtg gcaaaacaga
    15301 tcaaagaagg ggttgaatcg caggctattc tggtggccgg aaggcgaagc ggcatgcatt
    15361 tacgttgaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
    15421 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgc tatacgatgt cgcagagtat
    15481 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
    15541 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
    15601 gcacaacaac tggcgggcaa gcagtcgttg ctgattggcg ttgccacctc cagtctggcc
    15661 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
    15721 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
    15781 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
    15841 gccattgctg tggaagctgc ctgcactaat gttccggctt tatttcttga tgtctctgac
    15901 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
    15961 catctggtcg cattgggtca ccagcaaatc gcgctgttag caggtccatt aagttctgtc
    16021 tcggcgcgtc tgcgtctggc gggctggcat aaatatctca cacgcaatca aattcagccg
    16081 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
    16141 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
    16201 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
    16261 tacgacgata ccgaagacag ctcgtgttat atcccgccgt taaccaccat caaacaggat
    16321 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
    16381 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac ccttccgccc
    16441 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacaa
    16501 gtttcccgac tggaaagcgg gcagtgagcg caacacaatt aatgtgagtc agctcactca
    16561 ttaggcaccc cagtctttac actctatgtg tccggctcgt atgttgtgtg aaattgtgag
    16621 cggataacaa tttcacacag gatacagcta tgactatgat tacggattct ctggccgtcg
    16681 tattacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcggcac
    16741 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    16801 agttgcgaag cctgaatggc gaatggagct ttgcctggtt tccggcacca gaagcggtgc
    16861 cggaaagctg gctggagcgc gatcttcctg acgccgatac tgtcgtcgtc ccctcaaact
    16921 ggcagatgca cggttacgat gcgcctatct acaccaacgt gacctatccc attacggtca
    16981 atccgccgtt tgttcccgcg gagaatccga caggttgtta ctcgctcaca tttaatgttg
    17041 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt
    17101 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagccgt ttgccgtctg
    17161 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc
    17221 gctggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc
    17281 gtgacgtctc gttgctgcat aaaccgacca cgcaaatcag cgatttccaa gttaccactc
    17341 gctttaatga tgatttcagc cgcgcggtac tggaggcaga agttcagatg tacggcgagc
    17401 tgcgcgatga actgcgggtg acggtttctt tgtggcaggg tgaaacgcag gtcgccagcg
    17461 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg cggttatgcc gatcgcgtca
    17521 cactacgcct gaacgttgaa aatccggaac tgtggagcgc cgaaatcccg aatctctatc
    17581 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg
    17641 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
    17701 tgattcgcgg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
    17761 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt
    17821 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg
    17881 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc
    17941 cgcgctggct acccgcgatg agcgaacgca taacgcggat ggtgcagcgc gatcgtaatc
    18001 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg
    18061 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtacagtat gaaggcggcg
    18121 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc
    18181 agcccttccc ggcggtgccg aaatggtcca tcaaaaaatg gctttcgctg cctggagaaa
    18241 tgcgcccgct gatcctttgc gaatatgccc acgcgatggg taacagtctt ggcggcttcg
    18301 ctaaatactg gcaggcgttt cgtcagtacc cccgtttaca gggcggcttc gtctgggact
    18361 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg
    18421 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg
    18481 accgcacgcc gcatccggcg ctgacggaag caaaacacca acagcagtat ttccagttcc
    18541 gtttatccgg gcgaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg
    18601 agttcctgca ctggatggtg gcgctggatg gcaagccgct ggcaagcggt gaagtgcctc
    18661 tggatgttgg cccgcaaggt aagcagttga ttgaactgcc agaactgccg cagccggaga
    18721 gcgccggaca actctggcta acggtacgcg tagtgcaacc aaacgcgacc gcatggtcag
    18781 aagccggaca catcagcgcc tggcagcaat ggcgtctggc ggaaaacctc agcgtgacac
    18841 tcccctccgc gtcccacgcc atccctcaac tgaccaccag cggaacggat ttttgcatcg
    18901 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga
    18961 ttggcgatga aaaacaactg ctgaccccga tgcgcgatca gttcacccgt gcgccgctgg
    19021 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct
    19081 ggaaggcggc gggccattac caggccgaag cggcgttgtt gcagtgcacg gcagatacac
    19141 ttgccgacgc ggtgctgatt acaaccgccc acgcgtggca gcatcagggg aaaaccttat
    19201 ttatcagccg gaaaacctac cggattgatg ggcacggtga gatggtcatc aatgtggatg
    19261 ttgcggtggc aagcgataca ccgcatccgg cgcgggttgg cctgacctgc cagctggcgc
    19321 aggtcgcaga gcgggtaaac tggctcggcc tggggccgca agaaaactat cccgaccgcc
    19381 ttactgcagc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg
    19441 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc
    19501 agtggcgcgg cgacttccag ttcaacatca gccgctacag ccaacaacaa ctgatggaaa
    19561 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc
    19621 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga
    19681 gcgccggtca ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc
    19741 tatgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact
    19801 tttggatgtt cggtttattc tttttctttt acttttttat catgggggcc tacttcccgt
    19861 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt
    19921 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca
    19981 aactcgggct gcgcaaatac ctgctgtgga ttattaccgg catgttagtg atgtttgcgc
    20041 cgttctttat ttttatcttc ggaccactgt tacaatacaa cattttagta ggatcgattg
    20101 ttggtggtat ttatcttggc ttttgtttta acgccggtgc gccagcagta gaggcattta
    20161 tcgagaaagt cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg tttggctgtg
    20221 ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat aatcagtttg
    20281 ttttctggct gggttctggc tgtgcactca tcctcgccgt tttactcttt ttcgccaaaa
    20341 cggatgcgcc ctcttcagcc acggttgcca atgcggtagg tgccaaccat tcggcattta
    20401 gccttaagct ggcgctggaa ctgttcagac agccaaaact gtggtttttg tcactgtatg
    20461 ttattggcgt ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat ttctttactt
    20521 cgttttttgc taccggtgaa cagggtacgc gggtatttgg ctacgtaacg acaatgggcg
    20581 aattacttaa tgcctcgatt atgttctttg cgccactgat cattaatcgc atcggtggga
    20641 aaaatgccct gctgctggct ggcactatta tgtctgtacg tattattggc tcatcgttcg
    20701 ccacctcagc gctggaagtg gttattctga aaacgctgca tatgtttgaa gtaccgttcc
    20761 tgctggtggg ctgctttaaa tatattacca gccagtttga agtgcgtttt tcagcgacga
    20821 tttatctggt ctgtttctgc ttctttaagc aactggcgat gatttttatg tctgtactgg
    20881 cgggcaatat gtatgaaagc atcggtttcc agggcgctta tctggtgctg ggtctggtgg
    20941 cgctgggctt caccttaatt tccgtgttca cgcttagcgg ccccggcccg ctttccttgc
    21001 tgcgtcgtca ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc gcgagcgcct
    21061 tatccgacca acatatcagg gcggaatgat cgcaatgaac atgtcgatga cagaaagaat
    21121 aaaagcaggc aagctattta ccgatatgtg cgaaggctta cctgaaaaaa gacttcgtgg
    21181 aaaaacgtta atgtatgagt ttaatcactc gcatccatca gaagttgata aaagagaaag
    21241 tctgattaaa gaaatgtttg ccacggtagg ggaaaacgcc tgggtagaac cccccgtcta
    21301 tttctcttac ggttccaaca tctatatagg ccgcaatttt tatgcaaatt tcaatttaac
    21361 cattgtcgac gactacacgg taacgatcgg tgataacgta ctgattgcac ccaacgttac
    21421 tctttccgtt acgggacacc cggtacacca tgaattgaga aaaaacggag agatgtactc
    21481 ttttccgata acgattggca ataacgtctg gatcggaagt catgtggtta ttaatccagg
    21541 cgtcaccatc ggggataatt ctgttattgg cgcgggtagt gtcgtcacaa aagacattcc
    21601 accaaacgtc gtggctgctg gcgttccttg tcgggttatt cgcgaaataa acgaccggga
    21661 taagcaatat tatttcaaag attttaaagt tgagtcttca gtttaaataa gcaaaattgc
    21721 ctgatgcgct ccgcttatca ggcctacaat ttcagagatc tacgtaggcc ggataaggtg
    21781 ttcacgccgc atccggcata aactacgctc gcggtaaccc agccgattac cgtttgtaat
    21841 cgatggcttc tggctgctcc agatatacgg tggtttgtgc cggttgtgtg ctggcaatca
    21901 ccttgccgcc acgtaccgaa taacgtaccg gaacctgacg gcgcagcgca tcaaacccat
    21961 tttcagccgg caggataatc aggttggcgc tgtttccggc ggcaatgccg taatccttca
    22021 aattcaacgt cctggcgctg tggtgggtga ttaaattcag gccgtcgtta atctgcccgt
    22081 agcccatcag ctggcaaaca tgcagcccca tatgcagcac ttgcagcata ttcgccgttc
    22141 ccagcggata ccacggatcg aagacatcat cgtgaccaaa gcagacgtta atgccggact
    22201 ccagcatctc tttaacgcgc gtgatgccgc gacgttttgg atacgtatcg aaacgtcctt
    22261 gcagatgaat attgaccaac gggttggcga caaagttaat accggacatt ttcagcaaac
    22321 gaaacaggcg cgaagtatat gctccgttat aggaatgcat tgccgtagta tggctggcgg
    22381 tgactcgcgc gcccatgcct tcacggtgcg ccagggcagc aacggtttcg acaaagcgtg
    22441 attgctcatc atcaatctcg tcacagtgaa catcgatgag acggtcgtat ttttgcgcca
    22501 gggcgaaggt tttatgcagc gactccacgc catattcacg ggtaaattca aaatgcggaa
    22561 tcgccccaac aacgtctgcc cctaagcgta acgcctcttc cagcaacgct tcaccattgg
    22621 gatacgacaa aatcccttcc tgcgggaagg cgacgatttg tagatcaatc cacggcgcaa
    22681 cttcctgttt cacttccagc attgctttca gcgcagtcag cgttgcgtcc gaaacatcga
    22741 catgggtacg cacatgctga atgccgttgg caatctgcca tttcagcgtt tgccatgcgc
    22801 gttgtttcac atcgtcatga gttaataacg ctttgcgctc ggcccagcgt tcaatgcctt
    22861 caaacagcgt gccggactga ttccagttcg gttgtccggc ggtttgcgtg gtgtccaggt
    22921 gaatatgcgg ctccacaaac ggcggtaaaa ctaaaccttg ttcggcatcc aggctgtttt
    22981 cagttacagg catcacacca gattgcgtat caatggcgct gatttttccg tcgtgaagat
    23041 gaatctgcca cagcccctct ttgccgggta accgggcgtt aataattgtt tgtaaagcgt
    23101 tattcgacac tgttagcctc cacatgcgtc attgctgctg tcgttttacg attcaaaatc
    23161 gggttaagga tcagatagct cagcgcgcca cctaataccg cgttgaccgg aacaattccc
    23221 ggtaaccagt ggcctgcggc aatccccagc gcgaccgcca gaatcgccac ccaattgaca
    23281 ctcatcatac gcgtggtcgc aaagtgctca tagcggcgac ggttcatcag ataatcagcg
    23341 atgatcacgc cgcccaccgg aggaatagct gccgaaagga aggtcaacca gccgacaaaa
    23401 ttgttataca gccataatgc gcagaccgta ccgataatac cgttaatcac cgaaagggtt
    23461 ttgctcgaca taccggtaat gttggcgaaa cctaaacccg acgcatagag cgcgttatcg
    23521 ttggtggtcc agatattcag ccccagcacc acaatcgcag gcagcagcag gccctgagca
    23581 atcatcacat cagagatatc cgccatcccc agtgccgccg cgcccgctgc accgaaaata
    23641 aacatcaacg agttgccgag gaaaaaggcc accatcgcca ccagcaccgc cagtttggca
    23701 ttacgaccaa accggacaaa gtcagcggtg agcgtgcccg cactgataaa tgaccccaca
    23761 accagcgcca gcgcgacatt gaaatctaac ggttgtgcgg gaacgaccgc ttttaatgcg
    23821 tccaggccgc ccatgccgtt aacggccaac cacacggaat aaccgccaag acaggcgata
    23881 gccggaaccg caatcaccga aagaactgtc agcgccgaaa tgccaaagaa gacggtgacg
    23941 gtcatcagta aaccggagac ggcaatcagc aaattaatat ccagcccggt tgccttgccc
    24001 accggaatag caaacatcgc cacgccaacg ccaaaccagc caacctgggt gccgcccagc
    24061 agcagtgaag gcaaccatga gcctttaaca ccaaacgaga agcgagcaag aagatgggtg
    24121 gtcaggccgg tttttgcgcc aatgtaacca agaaatgaag tgtaaatacc gaggagaaga
    24181 ttaccgatga gaactgcgag gaagaaatca tgatagctaa gaccggttcc gagagtgccg
    24241 ccggtccaca tactggcgga aaagaaggtt aatcccagca tgacgaacgt caatgccaat
    24301 acccctttcc gcgccgactg cgggactggc ccctggctaa agttgttatc ttgcgacacg
    24361 aaattccccc tttttgctgt ttgaaaccca aaaaatccgc cgcattctat tcatctgaag
    24421 ataaaaagca atcgttttcg taggggaata tattttttac atgaatgagt gcaaaaaggc
    24481 ggggggaact tatctgtgta aaaacagtgt gtaggcatga taagacgcgt cagcgtcgca
    24541 tcaggcatct gcgcacggtg tcggatgcgg cgcgagcgcc ttatccgacc tactcttcca
    24601 tcgcctggcg gatctgatcc aacgacgccg gatcatcaat ggtcgtcaga tcccccggat
    24661 cgcgtccttc gcaaatcgcc tggatcgtgc ggcgcagcat ttttccggat cgcgtttttg
    24721 gcaattgcga gacaaaccag acgtgcgccg ggcggccaaa gttgccaatc tggctgtcta
    24781 ccagcgccat aattgccttc tcttgcaagt gcgccacctc acggtcttcc agactgtcgc
    24841 tctctttcgg aatgacaaac gccaccgcca cctgcccttt cagcgcatct ttcaccccaa
    24901 ccaccgccac ttcggcaacg cccggatgac tggagatact ctcttcaatc tcacgcgtac
    24961 ccagccgatg tccggcaacg ttaatcacat cgtcagtgcg cccgagaata aagtgataac
    25021 cgtcagcatc gcggatgccc cagtcaaaag tggcgtacac cggacgggaa aacagcgacc
    25081 agtacgtctt cacaaagcgg tcgtcgtcgc cccagatggt ctgaatacag cctggcggca
    25141 atggcccctc cactaccagc atccctttct cattgacgcc acacggttcg ccggtgactt
    25201 cattgagcaa ctgcacgtta tagccataca tcggcacgcc ggggcttccc agacgcgtcg
    25261 gtctgtcatc cagaccgcga gcaatcgcca taatcggcca gccggattcg gtctgccagt
    25321 agttgtcgat gaccggcaca tccagcgtat tgctcaccca actggcggtc ggctcgtcca
    25381 gcggttctcc agccagatag agcacttcca gcgacgaaag atcgtgtttg cgaatttcag
    25441 cggtagggaa ttttttcagc acgcgaatgg cggtcggcgc tgagaacatg cggctaacct
    25501 gatatttctc gacaattttc caccacacgc cgcagtccgg ccaggtcggc aatccttcgt
    25561 aaacgatagt cgccatcccc gccagcagcg gcgcgtaaac gatatacgaa tgccctacca
    25621 cccagccgat atccgaagca caaaagaaca cgccgcccgc tttgccgcca aaaatggtgt
    25681 ccatcgaggt cgccagcgcc accgcatatc cgccgacatc acgctgcaca cctttaggtt
    25741 tgccggtcgt gccggaggtg tagagaatgc aggaggtttc gttggattcc agccatgcca
    25801 ccggcacccg cgcgccgatg tgctgatggc gcaacgacgc gaaatcgaca tcccgcccgc
    25861 taacgcgcgc cattttcgcc agcccgcgat ccaccagtaa aacgtgacgc ggctgatgct
    25921 gtgcctgact tatcgcatcg tcgagcaatt ttttatacgg aatgatttta ccgccgcgcg
    25981 ccccggcatc agccgagaca atcagcaccg gtttagcgtc atcaattcgc gccgccacgc
    26041 tgtgcgaggc aaaaccccca aacaccaccg agtgaatagc accaatgcgc gcgcaggcca
    26101 gcagggtaat atgcgcttcg gcaatcatcg gcatatacac cagcacccga tcgccacgct
    26161 gcacgcccag cgagcgcagc attgacgcca ccgcattcac ttcgtcatgc agttggcgga
    26221 aggtaaaggt acgctcttcc tctgtttccg aagagacggc aatcaatgcc agcgcctctg
    26281 gctgtttctc cagccagcga tcgatagcgt tgtgacacaa gttggttcgg ccttcacaaa
    26341 accaacgggc aaacggcggg ttgctgtggt cgagcgtttg cgtaaagggc gtctgccagt
    26401 caatacgccg ggcctgctcg gcccagaact gctccggttc gttaatcgaa cgctgataaa
    26461 attcgctaaa agacataatg ctctcctgtt gaacttacgc cttaccgccg tttacttaaa
    26521 tgacgtacag gtcgagatac tcattgaccg gcatctgttc caggcgagtt ctgtcgagag
    26581 aaacctccag aatgcgctgc tgctggcgag tcgggaactg gcgcgcgaga ttgattttga
    26641 atttatcgac cagcttcgga atgccatcct gacggcggcg agcatgacca attgggtact
    26701 ccaccaccac ttcttcaaat cgcgtgccgt cggtgaactc aagggttatg gcattggcga
    26761 tggcgcgttt ttccgggtcg tggtagtcag cggtaaacac cggatcttca aagcaattga
    26821 tcttctcgcg cagggcgtcg atgcgtttat cttgcgcaac gttgtcctcg taatctgccg
    26881 ccgttaagcg cccgaacagc agcgggatcg ccaccatgta ctgaatgcag tggtcgcggt
    26941 cagccgggtt attgagcggc ccttttttgt cgatgatgcg aatacaggct tcgtgggtgc
    27001 gaatggtcac tttttcgata tccgccgccg ttttgcctgc tgcctgcatc tgttcataga
    27061 gcgtcatcgc tgcttcaact gccgtctggg agtggaactc cgccgggaag gagattttga
    27121 acagcacatt ttccatcacg taagaaccat acgggcgctg gaagcggaac gattcgcctt
    27181 taaaggagac gtcgtagaag ccccacacag gcgcagtcag ggctgacgga taacccattt
    27241 cgccggtttt cgccatcagt gccagacgta ccgcgcggga agtggcatcg cccgccgccc
    27301 aggatttacg cgtaccggtg ttcggcgcat ggcgataggt gcgcagcgac tgtccgtcta
    27361 cccatgccag cgaaacggcg ttgagaattt cctcgcgggt caggccgagc atttcggcga
    27421 ccacggcggt ggaagccact ttcactaaca gaacgtggtc gaggccgacg cggttaaagg
    27481 agttttccag cgcgatgcag ccctgaattt catgggcttt gatcattgcg gtcagcacct
    27541 gtttcatggt caacggcgct ttgccgctgg cgaccgcgtt gcgcgaaagc cagtctgccg
    27601 ttgccagaat gccgccgagg ttgtcggaag gatgccccca ctccgccgcc agccaagtat
    27661 cgttgaaatc gagccaacgg atcatcgcgc caatgttaaa tgccgcctgg acggggtcga
    27721 gctgaaactg agttccgggg acgcgcacac cgttgggtac gacggtgccg gggacaattg
    27781 gccccagcag ttttttacag gccggatatt cgagagcttc cagaccacag ccgagcgtgt
    27841 cgagcaggca gtaatgtgcg gtgtcgtagg ctactctgga gctgatttcg tagttcatca
    27901 cgtaatcgac gatatcaacg atttcacgat caaattccgg gcgaatgttg ttgatttgag
    27961 ctgacattgg gtacgtttcc ttatttttat tcgtagaggt ttactggcgc ttatccagcg
    28021 cgacaaactg gcggtcttcc ggtccaacgt aattggcgga aggacggata attttgttgt
    28081 cctgacgttg ttcgataatg tgcgccgccc agccagtgac gcgggcgata acaaacagtg
    28141 gtgtgaatat ctcggtggga acacccatca tgttgtagga aacagcggag aaccagtcga
    28201 gattggggaa catctttttg ctctcccaca tcactgtttc caggcgatcg gcgatgttgt
    28261 acatcttcag cgagccgcct tcctgcgaga gctgctttgc cacgcgtttg atcacctggt
    28321 ggcgcgggtc ggcgatggta taaaccggat gcccaaaacc aatgaccact tctttgtttt
    28381 ccacccgctt acggatatca gcttcggctt cgtccggcgt ttcgtagcgt tgctggatct
    28441 ccagcgacac ttcattcgcc ccgccgtgtt tcggcccgcg cagtgcgcca atcgcgccaa
    28501 taatggcgga atacatatca gagccagtgc ccgcaatcac ccggctggta aaggtggaag
    28561 cgttaaactc gtgttcggcg tacagcacca gcgagatatg catcgcctct tcccagcttt
    28621 gcgacggctt ttcgccgtgc agcagatgca ggaagtgacc gccgatagag tcgtcatcag
    28681 tttccggctg gatgcgttcg ccgttgtggc tgtagtgata ccagtagaga agaatcgaac
    28741 taagtgacgc cagcagtttg tcggcaatat cccgcgcacc agaaacagtg tgcccctctt
    28801 tttctggcag cgtgcagccg agcgcggaaa cgccggtgcg cataacatcc atcgggtgtg
    28861 acgccgccgg taaggcttcc agcacggtac gcacgttagc cggtaaacca cgcagggctt
    28921 tcagtttcgt tttgtaggcg gcgagttcgt cacgggttgg cagtttgccg tggatcagca
    28981 ggtgcgccac ttcttcaaat tcacaatgtt ccgccagatc aagaatatcg tagccacggt
    29041 aatgcaggtc gttgccgctt ttacccacgg tgcagagtgc cgtattgccc gccggaacgc
    29101 cggaaagtgc caccgatttt ttcggtttaa tgacatgggt actgttttgc aggatcgttg
    29161 tgtcgctcat attgtcctcg tcattgttat catttgtagg gtcgagattg ttgtcggatg
    29221 cgacgctatg cgtcttatcc gacctacagt tcagttcacc tcgtaggcct gataag
//
LOCUS       DADTFG010000051        29219 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.051, whole genome shotgun sequence.
ACCESSION   DADTFG010000051 DADTFG010000000
VERSION     DADTFG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.051"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..222
                     /locus_tag="J1138_002049"
     CDS             <1..222
                     /locus_tag="J1138_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBA8453852.1"
                     /translation="DLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELL
                     LEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            378..1913
                     /gene="gadC"
                     /locus_tag="J1138_002050"
     CDS             378..1913
                     /gene="gadC"
                     /locus_tag="J1138_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBA8453853.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2044..3363
                     /locus_tag="J1138_002051"
     CDS             2044..3363
                     /locus_tag="J1138_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HBA8453854.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3687..3905
                     /locus_tag="J1138_002052"
     CDS             3687..3905
                     /locus_tag="J1138_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA8453855.1"
                     /translation="MCLVDFGLRFNHKGRHYFKAANGEDIALSLSIGAAMFNGHPDYE
                     RLIQIADEALYIAKRRGRNRVELCKASL"
     gene            3930..6329
                     /gene="dosP"
                     /locus_tag="J1138_002053"
                     /gene_synonym="pdeO"
     CDS             3930..6329
                     /gene="dosP"
                     /locus_tag="J1138_002053"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBA8453856.1"
                     /translation="MKLTDADTAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKVW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTSFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGIETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(6518..6949)
                     /gene="osmC"
                     /locus_tag="J1138_002054"
     CDS             complement(6518..6949)
                     /gene="osmC"
                     /locus_tag="J1138_002054"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBA8453857.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            7294..7509
                     /gene="bdm"
                     /locus_tag="J1138_002055"
     CDS             7294..7509
                     /gene="bdm"
                     /locus_tag="J1138_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBA8453858.1"
                     /translation="MFTYYQPENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7611..7748
                     /gene="sra"
                     /locus_tag="J1138_002056"
     CDS             7611..7748
                     /gene="sra"
                     /locus_tag="J1138_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBA8453859.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            7905..9602
                     /locus_tag="J1138_002057"
     CDS             7905..9602
                     /locus_tag="J1138_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBA8453860.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASEMLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            9736..10746
                     /gene="adhP"
                     /locus_tag="J1138_002058"
     CDS             9736..10746
                     /gene="adhP"
                     /locus_tag="J1138_002058"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBA8453861.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            10932..11210
                     /locus_tag="J1138_002059"
     CDS             10932..11210
                     /locus_tag="J1138_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Killer protein"
                     /protein_id="HBA8453862.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            11210..11494
                     /locus_tag="J1138_002060"
     CDS             11210..11494
                     /locus_tag="J1138_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBA8453863.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(11723..12376)
                     /locus_tag="J1138_002061"
     CDS             complement(11723..12376)
                     /locus_tag="J1138_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA8453864.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(12369..13253)
                     /gene="fdxH"
                     /locus_tag="J1138_002062"
     CDS             complement(12369..13253)
                     /gene="fdxH"
                     /locus_tag="J1138_002062"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HBA8453865.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(13266..16313)
                     /gene="fdnG"
                     /locus_tag="J1138_002063"
     CDS             complement(13266..16313)
                     /gene="fdnG"
                     /locus_tag="J1138_002063"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15726..15728),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBA8453866.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKSTLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            16545..17426
                     /gene="yddG"
                     /locus_tag="J1138_002064"
     CDS             16545..17426
                     /gene="yddG"
                     /locus_tag="J1138_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBA8453867.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            17699..19087
                     /locus_tag="J1138_002065"
     CDS             17699..19087
                     /locus_tag="J1138_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HBA8453868.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            19182..22922
                     /locus_tag="J1138_002066"
     CDS             19182..22922
                     /locus_tag="J1138_002066"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBA8453869.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTREIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDTVAGVAFPYFGGIENPHFRSVKHDPV
                     LVRQLPVKNLTLADGTVCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYSATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDAHELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            22919..24463
                     /gene="narH"
                     /locus_tag="J1138_002067"
     CDS             22919..24463
                     /gene="narH"
                     /locus_tag="J1138_002067"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBA8453870.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHNPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            24463..25158
                     /gene="narW"
                     /locus_tag="J1138_002068"
     CDS             24463..25158
                     /gene="narW"
                     /locus_tag="J1138_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBA8453871.1"
                     /translation="MQILKVIGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            25158..25835
                     /gene="narI"
                     /locus_tag="J1138_002069"
     CDS             25158..25835
                     /gene="narI"
                     /locus_tag="J1138_002069"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBA8453872.1"
                     /translation="MQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQMLD
                     KRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLTL
                     IGGAGLLVRRLTNPRIRVTSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKLV
                     GWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRY
                     QIVRSRR"
     gene            25915..26808
                     /locus_tag="J1138_002070"
     CDS             25915..26808
                     /locus_tag="J1138_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBA8453873.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLTGETRAAIINALHLTEDDILQGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLSHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(26904..27749)
                     /gene="nhoA"
                     /locus_tag="J1138_002071"
     CDS             complement(26904..27749)
                     /gene="nhoA"
                     /locus_tag="J1138_002071"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBA8453874.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEDKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELVLVMAAFDTH
                     PEAGK"
     gene            27924..28493
                     /locus_tag="J1138_002072"
     CDS             27924..28493
                     /locus_tag="J1138_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBA8453875.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSSDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(28810..29025)
                     /locus_tag="J1138_002073"
     CDS             complement(28810..29025)
                     /locus_tag="J1138_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA8453876.1"
                     /translation="MFIRPVKPSDVEPLMDMLLDRDQFDQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCASEMMTNDV"
     gene            complement(29106..29219)
                     /locus_tag="J1138_002074"
                     /pseudo
     CDS             complement(29106..29219)
                     /locus_tag="J1138_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7097 a         7502 c         7364 g         7256 t
ORIGIN      
        1 gacctctctg aacgtctgcg tctgcgcggc tggcaggttc cggctttcac tctcggcggt
       61 gaagccactg acatcgtggt gatgcgcatt atgtgtcgtc gcggcttcga aatggacttt
      121 gctgaactgt tgctggaaga ctacaaagcc tccctgaaat atctcagcga ccacccgaaa
      181 ctgcagggta ttgcccagca gaacagcttt aaacacacct gataacgttt aacggtaacg
      241 gtgtcccgaa acgaagccgt ttcgggacaa tttccaaagt ctgttcactg gcattagcaa
      301 cggaaaatat tgttctgaat acgcttcaga acaaaacagg tgcggttccg acaggaatac
      361 cgttttaggg ggataatatg gctacatcag tacagacagg taaagctaag cagctcacat
      421 tactcggatt ttttgccata acggcatcga tggtaatggc tgtttatgaa taccctacct
      481 tcgcaacatc gggcttttca ttagtcttct tcctgctatt aggcgggatt ttatggttta
      541 ttcccgtggg actttgtgct gcggaaatgg ctaccgtcga cggctgggaa gaaggtggcg
      601 tcttcgcctg ggtatcaaat actctggggc cgagatgggg atttgcagcg atctcatttg
      661 gctatctgca aatcgccatt ggttttattc cgatgctcta tttcgtgtta ggggcactct
      721 cctacatcct gaaatggcca gcgctgaatg aagaccccat taccaaaact attgcagcac
      781 tcatcattct ttgggcgctg gcattaacgc agtttggtgg cacgaaatac acggcgcgaa
      841 ttgctaaagt tggcttcttc gccggtatcc tgttacctgc atttattttg atcgcattag
      901 cggctattta tctgcactcc ggtgcccccg ttgctatcga aatggatgcg aagaccttct
      961 tccctgactt ctctaaagtg ggcaccctgg ttgtatttgt tgccttcatt ttgagttata
     1021 tgggcgttga agcttccgca acccacgtca atgaaatgag caaccccggg cgcgactatc
     1081 cactggctat gttactgctg atggtggcgg caatctgctt aagctctgtt ggcggtttgt
     1141 ctattgcgat ggtcattccg ggtaatgaaa tcaacctctc cgcaggggta atgcaaacct
     1201 ttaccgttct gatgtcacat gtggcaccgg aaattgagtg gacggttcgc gtgatctccg
     1261 cactgctgtt gctgggtgtt ctggcggaaa tcgcttcctg gattgttggt ccttctcgcg
     1321 ggatgtatgt cacagcgcag aaaaacctgc tgccagcggc attcgctaaa atgaacaaaa
     1381 atggcgtacc ggtaacgctg gtcatttcgc agttggtgat tacttctatc gcgttgatca
     1441 tcctcaccaa taccggtggc ggtaacaaca tgtccttcct gatcgcactg gcgctgacgg
     1501 tggtgattta tctgtgtgct tatttcatgc tgtttattgg ctacattgtg ttggttctta
     1561 aacatcctga cttaaaacgc acatttaata tccctggtgg taaaggggtg aaactggtcg
     1621 tggctattgt cggtctgctg acttcaatta tggcgtttat tgtttccttc ctgccgccgg
     1681 ataacatcca gggtgactct accgatatgt atgttgaatt actggtcgtt agtttcctgg
     1741 tggtacttgc cctgcccttt attctctatg ctgttcatga tcgtaaaagc aaagcgaata
     1801 ccggcgtcac tctggagcca atcaacagtc agaacgcacc aaaaggtcac ttcttcctgc
     1861 acccgcgtgc acgttcacca cactatattg tgatgaatga caagaaacac taaccatgtc
     1921 tgaatggtta taagacaagg gagcgataat tcatcgctcc ctttttcgtg cttgttgtcg
     1981 tattgactta accgggataa gtacgagaat gagcgcacat ctgtttaccg gaaaccagca
     2041 catatggata tctgctcccg aaacgagaaa ttagcgatta gaagaccagc gatactggtt
     2101 gcactggcac ttttactgtg tagttgtaaa agcacgcctc cagagtccat ggtgacacca
     2161 ccagcgggct caaagccacc agccacgacg caacaatcgt cacaaccgat gcgtggcatc
     2221 tggctggcca cggtgtctcg cctcgactgg ccaccggttt cctcggttaa cattagtaac
     2281 cccaccagcc gggcacgtgt acaacaacag gcgatgatcg acaaactgga tcatctgcaa
     2341 cgtctcggca taaacacggt ctttttccag gtcaagccgg atggtactgc cctgtggcca
     2401 tcgaaaattt tgccgtggtc cgatcttatg accggtaaga ttggtgaaaa tccgggttac
     2461 gatccgctgc aatttatgct cgacgaagcc cacaagcgtg ggatgaaagt acacgcctgg
     2521 tttaacccct atcgcgtatc ggttaatacg aagcccggta ctatcaggga actgaatagc
     2581 actctgtctc aacaaccggc gagcgtctat gtgcaacacc gcgactggat cagaacgtcc
     2641 ggcgatcgct ttgtcctcga cccgggcatc cctgaggttc aggactggat cacatcaata
     2701 gtcgcggaag tggtttcccg ctatccagta gatggcgtgc agtttgatga ctatttctat
     2761 actgagtcac caggttcacg gctaaatgat aacgaaacgt accgcaaata cggcggcgca
     2821 tttgcgtcaa aagcagactg gcggcgcaac aatactcagc agttaattgc gaaggtatcg
     2881 cacaccatta aaagcattaa gccggaagtc gaatttggcg ttagcccggc aggcgtgtgg
     2941 cgtaaccgat cacacgatcc gctaggttcc gatacccgag gtgcggcagc ctatgacgaa
     3001 tcctacgctg atacccgtcg atgggtggaa caagggttgc tggattacat tgctccccaa
     3061 atttactggc cattctcacg gagtgccgcg cgttatgacg tgttggcaaa atggtgggcg
     3121 gatgtcgtta aaccgaccag gacccgcctg tatatcggta tcgccttcta taaagtgggt
     3181 gaaccttcaa agatagagcc agactggatg attaacggcg gcgtaccgga actgaaaaag
     3241 cagctcgatc ttaacgatgc tgtgccggaa attagcggca caatcttgtt ccgtgaggac
     3301 tatctgaata aaccgcagac gcaacaagcg gtcagctatc tgcaaagtcg ttggggcagt
     3361 taaactccca ttcttacttt gtaacaagcc ggtgctttac ccactggctt gttaccctct
     3421 tgaactatcc cactagcaat catgcataaa tacgtcaaat taaccacgca attaaccaca
     3481 cttagttaaa cagccataat ccccctaata aaaatgtatg aatatgaaaa aacatacgat
     3541 attcgctgtc accgaaaaat aattacgatg ggctatactc ttctgaagaa cagtagcgta
     3601 gattgaaact gtcctaagtc gcttatttct tttcagtata tcttcatatt tcaggaaaat
     3661 atggtgcagt tagtcgatct cgcacgttgt gtttggttga ttttggcctg cggtttaacc
     3721 ataaaggtcg acattatttt aaagccgcaa acggcgagga tattgccctc tcactttcca
     3781 tcggtgccgc catgtttaat ggtcatcctg actatgagcg cctcattcaa atagccgatg
     3841 aagctctgta tatcgccaaa agacgaggta gaaaccgtgt tgaactctgt aaagccagtc
     3901 tttagatgcg ccaggatgca gaggtaatca tgaagctaac cgatgcggat actgccgccg
     3961 atggcatttt tttccccgcc cttgagcaaa atatgatggg cgcggtgtta attaacgaaa
     4021 atgatgaagt gatgtttttc aaccccgccg cagagaagct ctgggggtac aaacgtgaag
     4081 aagtcattgg caataacatt gatatgctga ttccgcggga tttgcgtcct gcgcatcctg
     4141 aatacattcg tcacaaccgt gaaggtggta aagcgcgcgt cgaggggatg agtcgagagc
     4201 tgcagctgga gaaaaaagac ggcagtaaag tctggacccg ttttgcgcta tcgaaagtga
     4261 gcgccgaggg gaaagtttat tacctggcgc tggtacggga tgccagcgta gaaatggcgc
     4321 aaaaagaaca gacccgacaa ttgattattg ccgttgacca tctcgaccga ccggtgattg
     4381 tcctcgatcc ggaacgccat attgtgcagt gcaatcgcgc atttaccgaa atgtttggtt
     4441 actgcattaa cgaagccagc ggtatgcagc ccgatacact cctgaacatt ccagaattcc
     4501 ctgctgataa ccgtattcgt ttacagcagt tgctatggaa aaccgcccgc gatcaggacg
     4561 aatttctgct gttgacgcgc accggtgaaa aaatctggat taaagcctct atcagcccgg
     4621 tttatgacgt gctcgcgcat ctgcagaacc tggtaatgac attctcggat atcaccgaag
     4681 aaaggcaaat ccgccagctt gaaggcaata ttctcgccgc catgtgcagc agcccaccat
     4741 ttcatgaaat gggggaaatc atttgtcgta acatcgaatc tgttctcaac gaatcgcatg
     4801 tttcgctgtt cgcgctgcgc aacgggatgc caatacactg ggcgtcatct tcgcacggtg
     4861 cagaagttca aaatgcgcaa agctggtcag cgaccattcg tcagcgcgat ggcgcgcctg
     4921 cggggatcct gcaaattaaa acctcgtcag gagcagaaac cagcgccttt atcgaacgcg
     4981 tggcagatat cagccagcat atggccgcgc tggcgctgga acaggaaaaa agccgtcagc
     5041 atattgaaca actcatccaa tttgatccaa tgaccggtct gccaaatcgc aataacctgc
     5101 acaattacct cgatgacctg gtcgataaag ccgtctctcc ggtggtgtat ctcatcggtg
     5161 ttgaccatat tcaggatgtg attgatagcc ttggctatgc gtgggccgat caggcattgc
     5221 tggaagtggt caatcgcttt cgtgaaaaac tcaaaccgga tcagtatctc tgtcgtattg
     5281 aaggtacgtc ttttgtcctc gtgagcctgg aaaacgatgt cagtaacatt acccagatcg
     5341 ccgatgagct acggaatgtg gtcagcaagc cgataatgat tgacgataaa ccattcccgc
     5401 tcaccttgag cattggcatc agctacgacg tgggtaaaaa ccgcgattac ttgctctcca
     5461 ctgctcacaa tgcaatggat tttattcgca aaaatggcgg taacggctgg cagttcttca
     5521 gtccggcgat gaacgaaatg gtaaaagagc gtttggtttt aggcgcagcg ctaaaagaag
     5581 cgattagcaa taaccaactt aaactggttt atcagccgca aatcttcgca gaaacgggtg
     5641 aactgtacgg catcgaagcc cttgctcgct ggtacgatcc cctgcatggt catgtgcccc
     5701 cttcacggtt tattcctctc gcagaagaga ttggtgaaat cgaaaatatt gggcgctggg
     5761 tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt catatcccgg
     5821 cgttatccgt taacttgtct gcgctgcact ttcgcagtaa tcagctgcct aatcaggtat
     5881 ctgatgcaat gcacgcctgg ggtattgacg gccaccagct gacggtggaa atcacggaaa
     5941 gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg cgcgatatgg
     6001 gcatcggttt atcggtagat gattttggta caggcttttc cggattatcc cgcttagtca
     6061 gtcttccggt aacggaaatc aaaattgata aaagttttgt cgatcgttgt ctgaccgaaa
     6121 aacgcatcct tgccttactc gaagccatta ccagcattgg gcaaagcctc aatttaaccg
     6181 tcgtggctga aggcatcgaa accaaagagc aatttgagat gctacgcaag atccactgtc
     6241 gcgttattca gggatatttc ttttcccgcc ccctgcccgc cgaagaaatt ccaggctgga
     6301 tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact cgcgggtttt
     6361 tatttaacgt gacactgtca cttaaatact gtgatttccg ctacgtttag ttggacaacg
     6421 acaacgcata gccatgtcag aacaatccct tctcagcctg atgtgattgt gctgacgcat
     6481 caggcattca cgcgcctgac gcgtcatccg gcaacgctta cgatttcaac tggtaatcca
     6541 gcgtaatttc cgctttcagt acctgagaaa ccgggcatcc tgcttttgct ttctggataa
     6601 tgccgtcaaa ggtggaggca tcaatacccg gcaccgcaac ttcgctcttc agtgcgattt
     6661 tagtaatcgc aaaaccggca tccactttat ccagcgagac atcggcggtg gtatcaatcg
     6721 atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag aaacatgcgg
     6781 catgtgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca aaacgcgtgt
     6841 taaatccata cggctgttgg ttcagcacgc cgctctcagt agatactgtt cccttcccgc
     6901 gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt gttgctctcc
     6961 tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat aagcaggata
     7021 atccgaataa aatccggcga aaatataatt cctgcactta aattaaattt ccacaataga
     7081 taaccggaaa tatataactc aagagatatt gcatcaatat caatcaactc atcacaataa
     7141 taatacctat caatatcatg gagttaaaat ataaaaatca tcaaccagga ctaatcttaa
     7201 caacgaagcg gcaaatattt gccatgctga atgtgcttta taaatctgcg atccgtagca
     7261 gacaccataa atacacagac acggagaatc actatgttta cttattatca gccagaaaat
     7321 tcaacagcag aacccgctct ggttaatgcc attgagcaag gccttcgggc agagcacggt
     7381 gttgtcactg aagatgacat tctcatggag ctgaccaaat gggtggaagc ttctgataac
     7441 gacatcctca gtgatatcta ccagcaaacg atcaattatg tggtcagtgg tcagcaccct
     7501 acgctttaag gtgctatgct tgatcggcaa cctaatttag gggtttagca cgtgtttctt
     7561 cgctacggcg atgttgtcct taaaactagc aacaggattg aggagttaaa atgaaatcga
     7621 accgtcaggc acgtcatatt cttggactgg accataaaat ttctaaccag cgcaaaatag
     7681 ttaccgaagg tgacaaatcc agcgtggtaa ataatcccac cggcagaaaa cgccccgctg
     7741 aaaagtaatt cataaccatc agtcctcaat gacgattaaa caccattgcc tgcgcaatgg
     7801 tgtttttgtt tttatctgct ttatacttgg tacttgacgc cctggcggta atgcaaagac
     7861 gataaaagcc cccagggatg gatattcaaa aaagagtgag tgacatggaa ccaaaaacaa
     7921 aaaaacagcg ttcgctttat atcccttacg ctggccctgt attgctggaa tttccgttgt
     7981 tgaataaagg cagcgccttc agcatggaag aacgccgtaa cttcaacctg ctggggttac
     8041 tgccggaagt ggtcgaaacc atcgaagaac aagcggaacg agcatggatc cagtatcagg
     8101 gattcaaaac cgaaatcgac aaacacatct acctgcgtaa catccaggac actaacgaaa
     8161 ccctcttcta ccgtctggta aacaatcatc ttgatgagat gatgccagtc atttacaccc
     8221 caaccgtcgg cgcagcctgt gagcgttttt ctgagatcta ccgccgttca cgcggtgtgt
     8281 ttatctctta ccagaaccgc cacaatatgg acgatattct gcaaaacgta ccaaaccata
     8341 atatcaaagt gattgtggtg actgacggcg aacgtattct ggggcttggt gaccagggca
     8401 tcggcgggat gggcattccg atcggtaaac tgtcgctcta taccgcctgt ggcggcatca
     8461 gtccggccta tacccttccg gtggtgctgg atgtcggcac caacaaccaa cagctactta
     8521 acgatccgct gtatatgggc tggcgtaatc cgcgtatcac tgacgacgaa tactatgaat
     8581 tcgttgatga atttatccag gctgtgaaac aacgctggcc ggacgtactg ttgcagtttg
     8641 aagacttcgc acaaaaaaat gcgatgccgt tacttaaccg ctatcgcaat gaaatttgtt
     8701 cttttaacga tgacattcag ggcaccgcgg cggtaacagt cggcacactg atcgcagcca
     8761 gccgcgcggc aggtggtcag ttaagcgaga aaaaaatcgt cttccttggc gcaggttcag
     8821 cgggatgcgg cattgccgaa atgatcatcg cccagaccca gcgcgaagga ttaagcgagg
     8881 aagcggcgcg gcagaaagtc tttatggtcg atcgctttgg cctgctgacc gacaagatgc
     8941 cgaacctgct gcctttccag accaaactgg tgcagaagcg cgaaaatctc agtgactggg
     9001 ataccgacag cgatgtgctg tcactgctgg atgtggtgcg taatgtaaaa ccagatattc
     9061 tgattggcgt ctcaggacag accgggctgt ttacggaaga gatcatccgt gagatgcata
     9121 aacactgtcc gcgtccgatc gtgatgccgc tgtctaaccc gacgtcacgc gtggaagcca
     9181 caccgcagga cattatcgcc tggactgaag gtaacgcgct ggtcgccact ggcagtccgt
     9241 ttaatccagt ggtatggaaa gataaaatct accctatagc ccagtgcaac aacgccttta
     9301 ttttcccggg catcgggctg ggtgttattg cttccggcgc gtcacgtatc accgatgaga
     9361 tgctgatgtc ggcaagtgaa atgcttgctc agtattcgcc gctggtcctg aacggcgaag
     9421 gtctggtact accggaactg aaagatattc agaaagtctc ccgcgcaatt gcctttgcgg
     9481 ttggcaaaat ggcgcagcag caaggcgtgg cggtgaaaac gtctgccgaa gctctgcaac
     9541 aggccattga tgataatttc tggcacgccg aataccgcga ctaccgccgt acctccatct
     9601 aagcctgcgc ccggtagtga aggctaccgg gcaattttcc tctccctttt tcagatctca
     9661 tccatactgg gtagtggcga ataaatctca tttgcctcac ctgctatgca gaacatcatc
     9721 cgaaaaggag gaactatgaa ggctgcagtt gttacgaagg atcatcatgt tgacgttacg
     9781 gataaaacac tgcgctcact gaaacatggc gaagccctgc tgaaaatgga gtgttgtggt
     9841 gtatgtcata ccgatcttca tgttaagaat ggcgattttg gtgacaaaac cggcgtgatt
     9901 ctgggacacg aaggtattgg tgtggtggca gaagttggcc ctggtgttac ttcattaaaa
     9961 ccaggcgatc gtgccagcgt ggcgtggttc tatgaaggat gcggtcattg cgaatactgt
    10021 aacagtggta acgaaacgct ctgccgttca gttaaaaatg ccggatacag cgttgatggc
    10081 gggatggcgg aagagtgcat cgtggtcgcc gattacgcgg taaaagtgcc agatggtctg
    10141 gactcggcgg cggccagcag cattacctgt gcgggggtca ctacctacaa agccgttaag
    10201 ctgtcaaaaa ttcgtccagg acagtggatt gctatctacg gtcttggcgg tctgggtaac
    10261 ctcgccctgc aatacgcgaa gaatgtcttt aacgccaaag tgatcgccat tgatgtcaat
    10321 gatgaacagt taaaactggc aaccgaaatg ggtgcagatt tagcgattaa ctcacgcacc
    10381 gaagacgccg ccagaattgt gcaggagaaa gccggtggcg ctcacgcagc ggtggtgaca
    10441 gcggtagcta aagctgcgtt taactcggcg gtggatgctg tccgtgcagg tggtcgtgtc
    10501 gtggctgtcg gtctgccgcc ggagtctatg agcctggaca tcccacggct ggtgctggat
    10561 ggtattgaag tggtcggttc gctggtcggc acgcgtcagg atctaaccga agccttccag
    10621 tttgccgccg aaggcaaagt ggtgccgaaa gtcgccctgc gtccgttagc ggacatcaac
    10681 accatcttta ccgagatgga ggaaggcaaa atccgtggcc gtatggtgat tgatttccgt
    10741 cgctaagagg tctttgctgc gactgccatg ttcgggtcgc agcctgtttt gcgagacgct
    10801 ttccgcaatt tcactcttct tcttttcctc ccccatcttt cccctcacaa ttcgcgaaaa
    10861 cgtagcgaaa tttgttgcgc tgtacggaac aatagtgata gtgttccttt cgagggaaca
    10921 aggatgaaga gatgataatg aatttcagac acaagggatt gcgtgacttg tttcttctcg
    10981 gcaaaacctc cggcgttata ccgacgcaag tcaaacgatt acgccaccga ctcgctgtga
    11041 ttgatgcagc atgctgtctt gctgatatcg atatgcccgg ttaccgacta catccgttaa
    11101 gcggcgatcg cgatggaatt tgggcgatat ctgtctcggg caactggcga atcacatttg
    11161 aattcgtcaa tggcgatgca tacatactgg attacgagga ctatcactga tgaaaatggc
    11221 caatcatccc cgcccggggg acattattca ggaatcactg gacgaactta atgtcagcct
    11281 gcgcgagttt gccagagcaa tggaaattgc gccctcaacg gcaagccgat tgctgaccgg
    11341 aaaagcagct ttgacgccag aaatggcaat aaaactttcc gtggtgatcg gcagttcgcc
    11401 gcaaatgtgg ctgaatctgc aaaatgcctg gagtctggca gaggcagaaa aaacagtgga
    11461 tgtgtcgcga ctgcgccgtc tggttacgca ataaaaaaat ggcaccttat aaagcgccat
    11521 ttgaaactga tgacaaacgc aacattgccc gatgcgctac gcttatcagg cctacacgtt
    11581 cactgcaatc tattggattt gaacggtttg taggccggat aaggcgttca cgccgcatcc
    11641 ggcatgaaca aagtgcacgt tgtctacaat aaaaaaatgg cgccttttac agcgccattt
    11701 tcagcttaaa gctcaaaaga gattataacc cttcttcact ctcttttttc gcttctgcct
    11761 tctcgatttc acgataccag cgcggatggt gtttcttcgc ccagcgacgg ctcaccttcc
    11821 cttcgatcat ccctttaata gatcctttca cccagaatgc catatacata tggatcagga
    11881 tggcgtggat caggatgata cccgcagccg catggatcag caggctataa cggacaacct
    11941 gtattgggaa gtactgcgca aagtacggac gccagataat cactccggtc accagcagca
    12001 cgaaaatcat gctcatgatt gaccagaaca tcatcttttg cccggcgttg tacttaccga
    12061 catccgccac tttgtgctca ttgcctttta atacttcgac aatgttcaac agccacggaa
    12121 tatctttctt atccgggatg ttgtgatgca caaaacgcac aaacataaac atcagcgcga
    12181 cgaaaatcgc aatgccgaag aacgggtgca aaatgcgtcc catctgcggc gtaccgaagg
    12241 tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat cccggacagc gccaccagga
    12301 agaagcaaat caccacggtc cagtgacagg cgcgatcaat aaatttggtg cgcacaatca
    12361 ttttcgactt actcatgatt tccctcctcg tcatcgtcca cttccttatt cgggccaata
    12421 ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa agccagccgc tgccagcggt
    12481 ttcaacgcac ctttccacag gcttaccgag gtatcgatct tcggatcttt cggcagacca
    12541 tgatacagct ccggctgatc ggcatgatgc agcacgtaca taacgtgcgt accaccgacc
    12601 ccttccgggt tgtagacgcc agcatgttcg taaccacgcg ctttcagttt cgctacgcgc
    12661 tgttccgcca gctccagcat ctccttcttg gtgccgaagt ggatagcccc ggtcggacag
    12721 gttttcacac aagccggttc ctggccgacg ctgacgcgat cgacgcagag cgtgcattta
    12781 tatacccggt tatcctcttt gttgaggcgc ggaatattaa acggacaccc ggcaatgcag
    12841 taaccacagc cgatgcaatt ttccgactgg aaatcgacaa tcccgttagc gtactgaatg
    12901 attgcaccag cagacgggca cgccttcagg cagcccggat cttcacagtg catacagccg
    12961 tctttacgga tcagccactc cagcttgccg ttctgttcgg tttcgctaaa gcgcatcacc
    13021 gtccaggact tggcgctcag atcggcgggg ttatcgtaaa ccccgacgca gtgccccact
    13081 tcatcacgga tgtcgttcca ctccgaacac gccacctgac aggctttaca gccgatacag
    13141 gtggaaacgt cgataagttt cgcgacttct gctttgtaat cacgcacctg agaaggcggc
    13201 gtgatggagt tagttgcgga ccttttgata atgtcctgcg tttccatagc catctgttcg
    13261 cccccttacg ccttctcgat gtttactaag aacgctttat attccggcgt ttgcgagttt
    13321 gcatcaccga cattcggcgt cagagtgtta gcgatataac ctttacgcgc gacaccctca
    13381 aagccccagt ggattggaat acccaccgtt tcaacctgct gaccgtttac attcagcggt
    13441 ttcagacggc gcgttaccac agccaccgcg cggataaagc cacgcttgct ggagacagtg
    13501 acacgatcgc cattggcaat gcctttcgcc gccgccagcg tttcgctgat ttccacaaac
    13561 tgttccggct gagcaattgc gttgagcaat gcgtgcttgg tccaggtgtg gaagtgctcc
    13621 gtcagacgat aggtcgtacc cacatacggg aactgctctt ttttacccat ccgcagtgcg
    13681 tcttgttcat acagacgaac aaccgggtta gacaccacgt tcgggtgcaa cgggttagtg
    13741 cccagcggcg tttcaattgg ctcgtagtgt tccgggaacg gaccttccgc cattttgttg
    13801 atggcaaaca ggcgtcccat cccttccggc tgcatgataa acggcccggt tggcgtaccc
    13861 ggtgcggcat tgccgaagtc cggaatatcg ttacccgtcc acttgctacc gttccactgg
    13921 atcagcatcc gtttcggatc ccacggttta ccgttgatat ccgccgaagc acggttgtag
    13981 agtacgcgac ggttgagcgg ccacgcccaa gcccatccca gcgtattccc cagaccggac
    14041 gggtcagagt tatcgcggtt agccatctgg ttgccctgct cggtccagct accggtgtag
    14101 atccagcaag aagatgcggt tgtaccgtca tcacgcagat gcgcaaagct actcagcaac
    14161 tgacctttct tcgcaatcag cacgccatta gcgtcataga gatcttccag cgcatagccg
    14221 ttgttctctt tcgccacttc gtcagactgc ggttcgtgcg gctgcttgta gttccagctc
    14281 atcttcatca gcggttctac gcctttacca ccctcggcct ggtacagctc gcgcagatga
    14341 tggtagatac ccgccagaat ttcgccgtcg ttacgtgctt cgcccggcgc gtcctgacct
    14401 ttccagtgcc actgcaacca gcgaccggag ttagcgatag aaccatcttc ttcagcaaag
    14461 caggtcgaag gcagacggaa tacttcagtc tgaatagatg ccggatcgac atcgttcgac
    14521 tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca gcggatcgat aaccaccatg
    14581 tacttcagct tgctcaggca gctcaccact ttgtttttgt ccgggaagga cgcaaccggg
    14641 ttaaagccct ggcagaaata accggtgact ttaccttcat ccatcatgtt gaaatacttg
    14701 atgacgtcgt aggtctggtc ccacttcggc agccagtcgt agccccagtt gttctctttc
    14761 tgcgcggcat cgccgtagaa agatttcatc aggctaacga aaaacttcgg atagttgctc
    14821 cagtagttca cctgatcagc cagcgtcgat ttcggcgtgt tcgcttccag atacgactgc
    14881 aaatcaacct gtttttctga cggcagcgtc agataacctg gcaggctggt agagagcagg
    14941 cctaagtcag tcaggccctg aatgttggag tgaccacgca atgcgttcac gccgccaccg
    15001 gccataccca tgttaccgag cagcaactgg atcatcgcca tcgtacggat gttctgcgca
    15061 cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg tggttgtgcg atccggtgcg
    15121 ctggtggagg ccagcacttc acacactttc aggaagtcgg cttttggcgt accgcaaatg
    15181 ttttctacta cgtccggcgt gtagcgggaa acgtgcgctt tcagcaggtt ccatacacag
    15241 cgcggatgag tcagcgtgtc atcgcgtttc gcatagccgt tttcatcgaa ctgatagttc
    15301 caggacgact tatcgtattg acgtttttca gcgtcgtagc cgctgaacag accgtcttcg
    15361 aaagcaaaat catcacgcac cagcaggctg gcgttggtgt aatgcttaac gtattcggcg
    15421 ttgattttgt tgttttcgat caggtagcgc aaaacgccag acaggaacgt aatgtccgta
    15481 ccggaacgaa taggtgcgta aatatccgcc acagaagcgg tacgcgtaaa acggggatcg
    15541 acaacgatca aggttgcatc gttgttgttt ttcgcttcca tcgcccagcg gaaaccgacg
    15601 ggatgcgctt cagcagcgtt accgcccatc accattacga cgttagcgtt tttgatatcc
    15661 acccagtggt tggtcatcgc accgcgacca aatgttggag caagacttgc taccgttggt
    15721 ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc cgagggagcg ggcaaatttt
    15781 tgagtcagca tcccggtttc gttgctggca ccggaagcac acagcatacc ggtagaaagc
    15841 caacggttta ccgttacgcc ctgctcgttc ttttcaataa agttagcgtc acggtcggct
    15901 ttcatcagct tcgcaatacg ggagaatgct tcttcccagc taatgcgctg ccatttgtca
    15961 gaacctggcg cacgatattc cgggtagcgc agacggtttt cgctgttgac gtaatccagc
    16021 aaaccggccc ctttcgggca cagcgcacca cggcttaccg gatgatccgg gtcaccttca
    16081 atgtgataaa tcgcttctct ggcgtttttc gcgccatcac ccaggctata catcaatagc
    16141 ccgcaaccta cggaacagta tgtgcaggtg ttacggatct ctttcgcgcg taataatttg
    16201 tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc ccaatgctgc tactgttgtt
    16261 cccgccatac cgcccgcgca gattttaaaa aattgtctgc gactgacgtc cattgctttc
    16321 ctcttttttc aggggtatta ctgcgcgagg aaactaacag taaaattctg aatctttttg
    16381 cgcgaaatca aaaagaacac gtgtttgcca caaaataatc cgcctgcgca aattgaccta
    16441 cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc cagttaaaaa aatgctataa
    16501 aattcagctt aatttttaac ggtaagagag acaaaacagc gagcatgaca cgacaaaaag
    16561 caacgctcat agggctaata gcgatcgtcc tgtggagcac gatggtagga ttgattcgcg
    16621 gtgtcagtga ggggctcggc ccggtcggcg gcgcagctgc tatctattca ttaagcgggc
    16681 tgctgttaat cttcacggtt ggatttccgc gtattcggca gatcccgaaa ggctatttac
    16741 tcgccgggag tctgttattc gtcagctatg aaatctgtct ggcgctttcc ttagggtatg
    16801 cggcgacccg tcatcaggcg attgaagtgg ggatggtgaa ctatctatgg cccagcctga
    16861 caattctctt tgccattctg tttaatggtc agaaaaccaa ctggctgatt gtaccaggat
    16921 tattattagc cctggtcggc gtctgctggg tgttaggcgg tgacaatggg ttacattatg
    16981 atcaaattat taataatatc accaccagcc cattgagtta tttcctggcg ttcattgggg
    17041 cgtttatctg ggcagcctac tgcacagtaa cgaataaata cgcacgcgga tttaacggaa
    17101 ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt ttactatttt cttacgccac
    17161 aaccagaaat ggtatttagc acgcccgtca tgattaaact catctctgcg gcatttacct
    17221 taggatttgc ttatgctgca tggaatgtcg gtatattaca tggcaacgtc accataatgg
    17281 cggtaggttc gtattttacg cccgtccttt cctcagcgct tgcagccgtg ctgctcagcg
    17341 ccccgctgtc gttctcgttc tggcaaggcg cgctaatggt ctgcggcggt tccctgctct
    17401 gctggctggc gacacgtcgt ggctaaaata attgtcgggt ctacggcccg gcagttaatc
    17461 agataaattc tgacaatatt aaaaaataaa tttcaaatac cagaaggcca ttttagttac
    17521 atttagtaac acatatctgt ctgtttaatc cacattctgc ccccgcagtt acgctttctt
    17581 atctattctt ctaatcagca cttcacctga ttcatcctta attctaataa ctggctattg
    17641 cgccttacct cctgttttgt ctttttttca ttaccaatgt gtgcatgtga ggaacaatat
    17701 ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc gactggaaac cagaaaatcc
    17761 ggccttctgg gaaaataaag gaaagcatat tgcacgaaga aacctatgga tatcagtcag
    17821 ttgtctactt cttgccttct gtgtctggat gctatttagc gcagttactg ttaatctcaa
    17881 taaaatcggt tttaatttca ctaccgatca actcttttta ttaaccgcat taccctccgt
    17941 ttctggcgca ttattgcgtg tcccctactc ctttatggtg cctatattcg gtggacgccg
    18001 atggacggtt tttagtactg caatcctgat tattccttgc gtctggctcg gaattgccgt
    18061 gcagaatccg aatactcctt ttgggatatt tatcgttatc gctttgctat gcggttttgc
    18121 aggtgcgaac tttgcttcga gcatgggcaa tatcagtttc ttctttccaa aagccaaaca
    18181 agggagcgct cttgggatta atggcggatt aggaaactta ggtgtaagtg tgatgcagct
    18241 ggttgcaccg ctggtcattt ttgtacccgt atttgccttt ctcggcgtca atggcgtacc
    18301 gcaggccgac ggttcggtga tgtcgctggc gaatgccgca tggatttggg tgccattact
    18361 ggcgattgcc acgatcgccg cctggtcagg gatgaatgat atcgccagtt cacgcgcctc
    18421 aattgccgac cagctccctg tcttacaacg cctgcatctc tggctgctga gcctgcttta
    18481 tcttgccacc ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc tggcaaaaac
    18541 ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt ggcccattta tcggtgccat
    18601 cgcgcgatcg gttggtggtg ctatttccga taaattcggc ggcgtgcggg tgacgttgat
    18661 caacttcatt tttatggcga ttttcagcgc cctgctgttc cttaccttac cgggcacagg
    18721 ctccggtaat ttcatcgcat tttacgccgt atttatgggg ctgtttctga ccgcgggtct
    18781 gggaagtggt tctactttcc agatgatcgc cgtcatcttt cgccagataa caatttatcg
    18841 ggtgaagatg aaaggcggta gtgatgagca agctcaaaga gaagccgtca ccgaaacggc
    18901 agccgctctg ggctttatct cagccattgg cgcagtgggc ggctttttta ttccgcaggc
    18961 gtttggcatg tcgctcaata tgaccggctc tccggtgggc gcgatgaaag tgtttttaat
    19021 cttctacatc gtttgtgtgc tactgacctg gctggtttat ggtcggcgga agttcagcca
    19081 aaaataagag atcgatgcct gacacgtctt atcaggctac aaccgtgtcg catccgacaa
    19141 ggcatcagat cccaattcat tcctggagca ggagttatgt catgagtaaa cttttggatc
    19201 gctttcgcta cttcaaacaa aagggcgaaa cctttgccga tggtcacgga caggtgatgc
    19261 atagcaaccg cgactgggag gacagctatc gccagcgttg gcagttcgac aaaatcgtgc
    19321 gttccaccca cggtgttaac tgtacaggct cctgtagctg gaaaatctac gttaaaaatg
    19381 gtctggtgac cagggaaatc caacagaccg actacccgcg cactcgccct gacctgccaa
    19441 atcatgaacc tcgcggctgc ccgcgtggcg caagttactc ctggtatctc tacagcgcta
    19501 accgcctgaa atacccgctc attcgtaaac gactgattga actgtggcgc gaagccctca
    19561 agcaacacag cgatccggta ctggcgtggg catcgattat gaacgatccg caaaagtgcc
    19621 tgagctacaa acaagtgcgt ggacgcggtg gctttatccg ctccaactgg caggagctaa
    19681 atcagctgat tgccgctgct aatgtctgga ccatcaaaac ctatggcccg gatcgcgttg
    19741 ccggattctc accgattccg gcgatgtcga tggtttctta cgccgccgga acacgctatc
    19801 tgtcgctgtt aggtggcacc tgtttaagct tctacgactg gtattgcgac ctgccgcccg
    19861 cctcgccgat gacctggggt gagcaaaccg acgtaccgga atctgccgac tggtataact
    19921 ccagttacat catcgcctgg gggtctaacg taccgcagac acgtacgccg gacgcccatt
    19981 tctttaccga agtacgctac aaaggcacta aaaccatcgc cattacccct gactactctg
    20041 aagtggccaa attgtgcgac cagtggctgg caccaaagca aggcactgat agcgcgctgg
    20101 cgatggcgat gggccatgtg attttaaaag agtttcatct cgataatccc agcgattact
    20161 ttatcaacta ctgccgccgc tacagcgaca tgccgatgct ggtaatgctg gaacctcgcg
    20221 acgatggcag ctacgttccc gggcgcatga tccgcgcgtc tgacctggtg gatggactgg
    20281 gcgaaagcaa caatccgcag tggaaaaccg tggcagttaa taccgcaggt gaactggtgg
    20341 tgccgaacgg ttcgattggt tttcgctggg gagaaaaagg caaatggaac ctggaatcca
    20401 ttgccgccgg tacggaaacc gaattgtcgt taaccctgct cggtcaacac gacaccgtcg
    20461 caggcgtggc cttcccctac tttggcggca ttgaaaatcc gcactttcgc agcgtaaaac
    20521 atgatccggt actggtgcgc cagcttccgg tgaaaaatct gacgctcgcc gatggcactg
    20581 tctgtccggt ggtcagcgtt tatgatttgg tactggcgaa ttacggcctc gatcgcgggc
    20641 tggaagatga aaacagcgcg aaagattacg ctgaaatcaa accttatacc cctgcctggg
    20701 gcgagcaaat taccggcgtt ccgcgccagt atattgaaac catcgcccgt gaatttgccg
    20761 ataccgccca taaaacgcat gggcgctcga tgattattct cggcgcaggt gttaaccact
    20821 ggtatcacat ggacatgaac taccgcggga tgatcaatat gctgatcttc tgcggttgtg
    20881 tcgggcaaag cggtggcggc tgggcacact atgtcggtca ggaaaaactg cgcccgcaaa
    20941 ccggctggct gcctctggcc tttgcgctcg actggaaccg accaccgcgc cagatgaaca
    21001 gcacctcgtt tttctacaat cactccagcc agtggcgcta tgaaaaagtc accgcgcagg
    21061 aattgctctc accgctcgcc gatgccagta aatacagcgg tcacctgatt gatttcaacg
    21121 ttcgcgccga acgtatgggc tggctacctt ctgcgccgca gctggggcgt aacccgctcg
    21181 ggattaaagc tgaagccgac aaggccggat tatcccccac agaatttacc gcccaggcgc
    21241 tgaaatcggg cgatttacgc atggcctgcg aacaaccaga tagcggcagc aatcatccgc
    21301 gtaatttgtt tgtctggcgt tctaacctgc ttggctcctc cggcaaaggc cacgagtata
    21361 tgcagaagta tctgctggga accgaaagtg ggattcaggg tcaggaactc ggtgccagcg
    21421 acgggatcaa accagaagaa gtcgagtggc aaactgcggc gattgaaggc aaactcgacc
    21481 tgctggtgac gctcgacttc cgtatgtcca gtacctgcct gttctccgat atcgttctgc
    21541 ccaccgccac ctggtacgaa aaagacgata tgaacacctc ggatatgcat ccgtttattc
    21601 atcccctatc tgcggcggtc gatccggcgt gggaatcacg cagcgactgg gaaatctaca
    21661 aaggtattgc caaagccttt tcgcaagtgt gcgtgggcca tcttggcaaa gaaaccgacg
    21721 tggtattaca accactgctg catgactccc cggcagagct ttcacagccg tgtgaagtgc
    21781 tcgactggcg caaaggcgaa tgcgatctga ttccaggcaa aaccgcgccg aatattgtgg
    21841 cggtggagcg cgactactct gctacctatg aacgctttac ctcgctcggg ccattgatgg
    21901 acaaacttgg caacggcggt aaagggattt cgtggaatac ccaggatgaa atcgatttcc
    21961 tcggtaaact caattacacc aagcgtgatg gcccggcaca ggggcgtccg ctgattgata
    22021 ccgcgattga tgcctcagaa gtgattctgg cgctggcacc agaaaccaac ggccatgttg
    22081 cagtcaaagc gtggcaggcg ctgggcgaga tcaccggacg cgaacatacc catctggcgc
    22141 tgcacaaaga ggacgagaag attcgctttc gcgatattca ggcgcagccg cgtaaaatta
    22201 tctccagccc cacctggtct ggtctggaaa gcgatcacgt ctcctataac gcgggataca
    22261 ccaacgttca tgagttaatt ccgtggcgca cgctgtcggg acgccagcaa ctctatcagg
    22321 atcatccgtg gatgcgtgct tttggtgaaa gtctggtggc ttatcgcccg cctatcgaca
    22381 cccgtagcgt cagtgaaatg cgccagatac cgccaaacgg cttcccggag aaagccctta
    22441 acttcctgac accgcaccag aaatggggca ttcactcaac ctacagtgaa aacctgctaa
    22501 tgctgacgct ctctcgcggt ggaccgattg tctggatcag cgaaaccgat gcccatgaac
    22561 tgaccattgt cgataacgac tgggtcgaag tgtttaacgc caatggcgcg ctgacggccc
    22621 gcgcggtggt cagccaacgt gtaccgccgg gcatgaccat gatgtatcac gcccaggaac
    22681 gcattatgaa tattcctggt tcggaagtaa ctggcatgcg cggcggcatt cataactcgg
    22741 ttacccgcgt ttgcccgaaa ccaacgcata tgattggcgg ttacgcgcag ctggcctggg
    22801 gctttaacta ctacggcacc gtcggctcga accgcgacga attcattatg atccgcaaaa
    22861 tgaagaacgt taactggctg gatgatgaag gtcgcgatca ggttcaggag gcgaaaaaat
    22921 gaaaatacgt tcgcaagtcg gcatggtgct taacctcgat aaatgtatcg gctgccatac
    22981 ctgttcggtg acctgtaaaa atgtctggac cggacgcgaa ggcatggagt acgcatggtt
    23041 taacaacgtc gaaaccaaac cgggcattgg ttatccgaaa aactgggaag atcaggatga
    23101 gtggcaaggt ggctgggtgc gtgatgtgaa tggcaagata cgcccgcgtc tgggcagcaa
    23161 gatgggcgta ataaccaaaa ttttcgccaa cccggtagtg ccgcagattg acgattacta
    23221 cgaacctttc accttcgact acgaacattt gcatagcgcg ccggaaggca aacatattcc
    23281 caccgcccgt ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc
    23341 aaactgggaa gaactgctgg gcggcgaatt tgaaaaacga gcccgcgacc gcaacttcga
    23401 ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt atctgccgcg
    23461 cttgtgcgaa cactgcctta accccagttg tgtagcgacc tgccccagcg gcgctatcta
    23521 caaacgcgaa gaagacggca ttgtgctgat cgatcaggat aaatgccgtg gctggcgttt
    23581 gtgcattagc ggttgcccat acaaaaaaat ctacttcaac tggaaaagcg gtaagtcaga
    23641 aaaatgtatt ttctgttatc cgcgaattga gtccggtcag ccaacagtct gctccgaaac
    23701 ctgcgtgggt cgcattcgtt atcttggcgt gctgctctac gacgccgacc gcatcgaaga
    23761 agcagcgagt accgaacgag aggtcgatct ctatgaacgc cagtgcgaag tgttcctcga
    23821 tccgcacaat ccctcggtga tcgaagaagc cctgaaacaa ggtattccac aaaacgtgat
    23881 tgacgctgcc cagcgttcgc ctgtctacaa aatggcgatg gactggaaac tggcgctacc
    23941 gctgcaccct gaatatcgca ccctgccgat ggtctggtac gttcctccgc tgtcaccgat
    24001 tcagtcctac gctgatgcgg gcggcttgcc gaaaagcgaa ggcgtgctgc ccgccatcga
    24061 aagcctgcgt attccggtgc aatatctcgc caatatgttg agtgccggtg ataccggtcc
    24121 ggtactgcgg gcgctgaaac ggatgatggc gatgcgccac tatatgcgtt cacaaaccgt
    24181 ggaaggcgtt actgatactc gtgccatcga cgaagtaggc ctgagcgtcg cccaggtcga
    24241 agagatgtat cgttacctcg ccattgccaa ctatgaagat cgtttcgtca tcccgacaag
    24301 ccatcgggaa atggcgggcg atgccttcgc agaacgcaac ggctgcggtt ttacctttgg
    24361 cgacggttgc cacggttcgg acagcaaatt caacctgttc aacagtagcc gtatcgatgc
    24421 catcaacatc accgaagtgc gcgacaaagc ggagggcgaa taatgcagat cctcaaagtg
    24481 atcggcctgt tgatggagta cccagacgag ttgttgtggg agtgtaagga cgacgcgctg
    24541 gcgcttatcc gccgcgacgc gccgatgctc accgattttg cactcgacct gctcaacgcc
    24601 ccgctgctgg ataaacaggc cgaatggtgt gaagtgtttg accgcgggcg caccacgtcg
    24661 ctgctgctgt ttgaacatgt tcatgccgag tcccgcgatc gcggccaggc aatggtggat
    24721 ctgctggcgg agtatgaaaa ggtcggtctg caactgaatt gtagggaact gcccgattat
    24781 ctaccgctgt atctggagta tttaagcgta cttccggacg atcaggcgaa agaaggatta
    24841 ctcaacgttg cgccgatcct cgccctgctt ggcggacgct taaaacaacg cgaggcaccg
    24901 tggtacgcgt tatttgacgc cctgttgcaa ctggcaggaa gccctctgtc aagtgacagt
    24961 gtcacaaaac agattcgcag tgaagagcgt gacgacaccc gccaggcgct ggatgcggtg
    25021 tgggaagaag aacaggtgaa gtttattgaa gataacgcca cggcgtgtga cagctcgccg
    25081 cttaatcaat atcagcgacg ctttagccag gatgtcgcgc cgcagtatgt cgacatcagt
    25141 gcgggaggtg ggaaatgatt cagtatctga acgtcttttt ttacgatatc tacccgtact
    25201 tgtgtggcac ggtttttatc cttggaagct ggctgcgcta cgattacggg cagtatacct
    25261 ggcgtgccgc ctccagccag atgctggata aacgcgggat ggtgctatgg tcgaacttgt
    25321 tccatatcgg cattctgggg atcttcttcg gtcatctgtt cggcatgtta acgccgcact
    25381 ggatgtacgc gtggtttctg ccggtggcgg taaaacagca gatggcgatg atcctcggcg
    25441 gaatatgcgg ggtattgacg ctgattggcg gtgcggggct actggtgcgt cgtttaacaa
    25501 atccgcgtat cagggtgacg tcttctggtg cggatatcct gatcctcgcc attttgttaa
    25561 ttcagtgcat tcttgggtta accaccattc ctttttcagc acaacatccg gacggcagtg
    25621 aaatgatgaa gctggtgggc tgggcgcaaa gcattgtcac tttccagggc ggtgcatcta
    25681 cacatcttga tggtgtcgcc tttatattcc gcgttcatct ggtgcttgga atgaccatct
    25741 tcctgctctt cccgttcacc cgactggtgc atgtctggag cgcgccgttt gagtacttta
    25801 ctcgtcggta tcagattgtg cgttcgcggc gttaattctc acgcgggccg gatagccctt
    25861 cgctctccgg ccacttgcag gctatgatag ttctccagcc acaggagaac attaatgaaa
    25921 ccgcaggttt accacgtcga tgcttttacc tctcaaccat ttcgcggcaa ttctgccggt
    25981 gttgttttcc ccgccgataa tctcagtgaa gcgcaaatgc agcttatcgc ccgcgagtta
    26041 ggtcattcgg aaactgcttt tctgctgcac agcgacgaca gcgatgtgcg catccgctac
    26101 tttacgccaa cggttgaagt tccgatttgt ggtcacgcga cagtagctgc gcactatgta
    26161 cgtgcaaagg tgttaggttt aggaaattgc acggtctggc aaacatcgct ggcaggaaaa
    26221 catcgtgtga ctatcgaaaa gcaaaatgat gattatcgta tttcgctgga acaaggtact
    26281 ccaggctttg agccaccact gacaggtgaa acacgtgcag cgattatcaa cgcgcttcat
    26341 ctcactgaag acgatatttt gcaaggcttg ccaatccagg tggctaccac cggacactca
    26401 aaagtgatga ttccactgaa gccggaagtg gatatcgacg ccctttcgcc tgaccttgct
    26461 gcgctgaccg caatcagcaa gcagattggc tgcaatggtt tcttcccgtt ccagatccgc
    26521 ccaggcaaaa gcgaaaccga cggtcgcatg ttctcgcctg cgattggtat tgtggaagat
    26581 ccggtgaccg ggaatgccaa tggtccgatg ggcgcatggt tagtacatca caacgtatta
    26641 tcccacgatg gcaacgtgtt gcgtgttaaa ggccatcagg ggcgcgcatt ggggcgtgat
    26701 ggtgtaattg atgtgacggt gacaattcga gataaccaac cggagaaagt taccatttcc
    26761 ggcacggcgg tgattctatt ccatgctgaa tgggccattg atttttgatg ccgttgcctg
    26821 atgcgcttcg cttatcaggc ctacaaccgg actaatgtag gccggataag gcgtttacgc
    26881 cacatccggc aacctgacat aaattatttt cccgcctccg ggtgcgtatc aaacgccgcc
    26941 atcaccagca ctaactcatc cacagtaaag ccatgtttcg catcatctac gcccagacca
    27001 aactgttcct gcatcacggc atataacgac gccacatctg gtagatttcg ctgctcaact
    27061 gcgtgcccat tttcataatg ggtaaaatga aaattggtca gcgttagctt gccaccgtcc
    27121 ggcaaatggc gacacatcag caaatgatgg cgaaaatgcg actgcggcca gtgcgccgac
    27181 cagaaattgc ccatcacata atcgctttgt tgctgctcgc agagatcaaa acggtacatc
    27241 gactgccagt gctgatgatg attaaactgc aatacccaat catcaccctc ttgcaacaac
    27301 cgatactctc cgtgtggcgt ggtttgcatg atatccggca ttaaacgaat cggcgcggtt
    27361 agcgtctgcc caccgaaacc gacatcagca atccattttt ccccttccag ttccaccaac
    27421 agcaaacgat gggtgcgcgg cggtaatgct ggcggatttg ataacactac gcgacctaac
    27481 aagctgcgaa cgttaaaccc cagctcgcgt aacacccgct caaacacgcc attctgctca
    27541 aaacagtaac cgccacgacg ggctatcacc agtttatctt ccagcgattg atcatcaagc
    27601 tgtatttccc tcggcagcaa aacgtcgaga ttttcaaacg gaatggtgca attgtgtttc
    27661 aggtgcaatg cacgcagcgt atcaatattg gccgcagcag ctcccaacca gttaatacgg
    27721 gcaaaataat gattcagaat gggcgtcatc aggcgtttcc cttagtggtt ttctcaactt
    27781 ataacctgga atagacgccg cactggctat tttgtgcgat cgcggacatg catcacgttc
    27841 gcaaatcgcc aatcccgtta atttcgttac ttattgcgtt acgagctacg cttttaaaac
    27901 ccccttcata gcgaggcgat atcgtgagcc aattcattcc cgtcgaatta caccatgcca
    27961 gccgtctgtt aaatcatggc ccaactgtca tgatcaccag ttccgatgag caatcccagc
    28021 ggcgtaacat tatggcagca gcttggtcga tgcccgtaga gtttgaaccg ccacgcgtgg
    28081 cgattgtggt agataaatcg acatggacca gggagttgat tgaacgtaac ggtaagtttg
    28141 gcatcgttat cccgggcgtt gcggcgacta actggacgtg ggcggtggga agtgtgtcgg
    28201 gtcgtgaaga agataaattt aattgctacg gcattccggt agtgagaggc ccggtgcttg
    28261 gtttgccgct ggtcgaggaa aaatgtctgg cgtggatgga gtgtcgcctg ctacctgcaa
    28321 cttccgcgca agaaaaatac gatacgctat ttggcgaagt ggtatcagca gcggcagacg
    28381 cacgggtatt tgtcgaaggc cgctggcagt ttgatgatga taagctcaat acgttgcatc
    28441 atttaggtgc tggcacgttt gttaccagtg gtaagcgagt taccgcgggc taaagaatca
    28501 tatgcagcaa ccaagttgtt taagcaatgt ttccatatga gttaaagcat attttatctc
    28561 acgattgcat atttttagtg aagattaatt tatcctcacc aacatcatag taattgcgta
    28621 ttatcgcctc attgataaag ccctgttttg tataaaatgc gcgggcggca ctaaaatcga
    28681 tatggctggc ggtctctact aatagtagtc ttgcctgttt aattgtgagc tcttttctat
    28741 ttcgttaatc agcagatgtc tcactccctg gcgctgatgg gaaggactga tccagagcat
    28801 taataaattt cagacatcat ttgtcatcat ttcagaagca cagtaggcaa tccctgccag
    28861 tcccaagtgt tctgcactaa accataaatc ggcagattga cctgagaaat aatgtgttag
    28921 tgttttttgt acatgatgaa ggccatcttg atcaaattga tccctgtcaa gcagcatatc
    28981 cattaatggt tcaacatctg aaggtttgac tggtcgtata aacatggaac tccttattta
    29041 ttgatgaata tttgaaaatt gtttgcatgg aacgcggtta atctgaattt tcagcggaag
    29101 tttcactcaa aggctttatt aatccgctcc ttatgtcctt ttacagcaaa taaataatta
    29161 cctccctgtt tttgtatctt ctctgcaata tctttctggc aacccatcgc atcagttgt
//
LOCUS       DADTFG010000052        29211 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.052, whole genome shotgun sequence.
ACCESSION   DADTFG010000052 DADTFG010000000
VERSION     DADTFG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.052"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(153..1628)
                     /gene="ampG"
                     /locus_tag="J1138_002075"
     CDS             complement(153..1628)
                     /gene="ampG"
                     /locus_tag="J1138_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBA8453877.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLM"
     gene            complement(1672..2250)
                     /locus_tag="J1138_002076"
     CDS             complement(1672..2250)
                     /locus_tag="J1138_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA8453878.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2555..2872
                     /gene="bolA"
                     /locus_tag="J1138_002077"
     CDS             2555..2872
                     /gene="bolA"
                     /locus_tag="J1138_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBA8453879.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3216..4514
                     /gene="tig"
                     /locus_tag="J1138_002078"
     CDS             3216..4514
                     /gene="tig"
                     /locus_tag="J1138_002078"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBA8453880.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTEEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4760..5383
                     /gene="clpP"
                     /locus_tag="J1138_002079"
     CDS             4760..5383
                     /gene="clpP"
                     /locus_tag="J1138_002079"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBA8453881.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5509..6783
                     /gene="clpX"
                     /locus_tag="J1138_002080"
     CDS             5509..6783
                     /gene="clpX"
                     /locus_tag="J1138_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBA8453882.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6971..9325
                     /gene="lon"
                     /locus_tag="J1138_002081"
     CDS             6971..9325
                     /gene="lon"
                     /locus_tag="J1138_002081"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBA8453883.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9534..9806
                     /gene="hupB"
                     /locus_tag="J1138_002082"
     CDS             9534..9806
                     /gene="hupB"
                     /locus_tag="J1138_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBA8453884.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9998..11869
                     /gene="ppiD"
                     /locus_tag="J1138_002083"
     CDS             9998..11869
                     /gene="ppiD"
                     /locus_tag="J1138_002083"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA8453885.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12020..12391
                     /locus_tag="J1138_002084"
     CDS             12020..12391
                     /locus_tag="J1138_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453886.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12497..12895
                     /locus_tag="J1138_002085"
     CDS             12497..12895
                     /locus_tag="J1138_002085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HBA8453887.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12947..13642)
                     /gene="queC"
                     /locus_tag="J1138_002086"
     CDS             complement(12947..13642)
                     /gene="queC"
                     /locus_tag="J1138_002086"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBA8453888.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13707..15407)
                     /locus_tag="J1138_002087"
     CDS             complement(13707..15407)
                     /locus_tag="J1138_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBA8453889.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTVL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPDWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15507..16325
                     /gene="cof"
                     /locus_tag="J1138_002088"
     CDS             15507..16325
                     /gene="cof"
                     /locus_tag="J1138_002088"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBA8453890.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16478..16936
                     /locus_tag="J1138_002089"
     CDS             16478..16936
                     /locus_tag="J1138_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBA8453891.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16966..18738
                     /locus_tag="J1138_002090"
     CDS             16966..18738
                     /locus_tag="J1138_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBA8453892.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIGQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            18731..20512
                     /locus_tag="J1138_002091"
     CDS             18731..20512
                     /locus_tag="J1138_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBA8453893.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQYALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20693..21031
                     /gene="glnK"
                     /locus_tag="J1138_002092"
     CDS             20693..21031
                     /gene="glnK"
                     /locus_tag="J1138_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBA8453894.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21061..22347
                     /gene="amtB"
                     /locus_tag="J1138_002093"
     CDS             21061..22347
                     /gene="amtB"
                     /locus_tag="J1138_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBA8453895.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTVPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22396..23256)
                     /gene="tesB"
                     /locus_tag="J1138_002094"
     CDS             complement(22396..23256)
                     /gene="tesB"
                     /locus_tag="J1138_002094"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBA8453896.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23474..24046
                     /locus_tag="J1138_002095"
     CDS             23474..24046
                     /locus_tag="J1138_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453897.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24077..24388)
                     /locus_tag="J1138_002096"
     CDS             complement(24077..24388)
                     /locus_tag="J1138_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBA8453898.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            24551..24647
                     /gene="ffs"
                     /locus_tag="J1138_002097"
     ncRNA           24551..24647
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="J1138_002097"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24767..25120
                     /locus_tag="J1138_002098"
     CDS             24767..25120
                     /locus_tag="J1138_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBA8453899.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25162..26712)
                     /gene="pdeB"
                     /locus_tag="J1138_002099"
     CDS             complement(25162..26712)
                     /gene="pdeB"
                     /locus_tag="J1138_002099"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBA8453900.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHSQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26876..27346)
                     /locus_tag="J1138_002100"
     CDS             complement(26876..27346)
                     /locus_tag="J1138_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453901.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27462..28013)
                     /gene="maa"
                     /locus_tag="J1138_002101"
     CDS             complement(27462..28013)
                     /gene="maa"
                     /locus_tag="J1138_002101"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBA8453902.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28186..28404)
                     /gene="hha"
                     /locus_tag="J1138_002102"
     CDS             complement(28186..28404)
                     /gene="hha"
                     /locus_tag="J1138_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBA8453903.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28430..28804)
                     /gene="tomB"
                     /locus_tag="J1138_002103"
     CDS             complement(28430..28804)
                     /gene="tomB"
                     /locus_tag="J1138_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBA8453904.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7389 a         6989 c         7851 g         6982 t
ORIGIN      
        1 gaatcgtgta agaaaaatag aattcataaa ttgcgtcaat aatttccaaa cgctaagccg
       61 cacaaaagaa caaatattaa taagcgatgc tgatgtttat ttagccgtaa taattacggc
      121 gagtgattac tacagctaaa taatattaca gattacatca gatgtgtttt tcgtagcgcc
      181 agataatcca gcaaaccacc aagcacaacg ccagaaaggg cgactaaaac cccgacttcc
      241 agcagcgcag gcaggaaaga gaagtgcgtc aaatccagcg cgtccatcgt caacagcagt
      301 aaccacacgg ccaacaggct gatgcccgcc gccagtgtcc acatggcaaa ggcataacct
      361 gcaggatatt cggtacggga gataaagttg tcatttactc gtgtatattc aagcgtctgg
      421 cggcaaacca gcagcaaaat aagccctggt acggcagcgg cgacggagaa tagatagaat
      481 gtcgaccagc cgtgtgcttc aacaaaccaa cccgccacgg ggccgacata aactcgccct
      541 acagcagaaa gcgctgagag cagggcaaat tgagtagcgg aaaatgactt attacacagc
      601 gtcattaaca gcgcgacaaa ggctgatgta cccatcccgc cacagaggtt ttcgaaaaag
      661 acggctgcac ccatgctgta gagatgctta tcagtaatcg acagcagcca gtaaccggcg
      721 ttagatgcac cttgtaaaat gccgaaaatc agcagtgcac ggaacagcga caggcgctgc
      781 atcaaaatcc caccgtacaa tgcgccaaca atggtcgcca gtaagccaag cgttttgtta
      841 accacgccta cttcacccgc atcaaacccg acgccgcgaa tcaaaaacgt ggttgtcagg
      901 ctcatagcga atgcgtcgcc cagcttatac agcacgataa gaagcaaaat aagccaggca
      961 ttattgcgac caaagaaatc tcgcagaggt gcaacaaccg cttgttccag cgttttgggc
     1021 acaggaatgg tgtcggtggg ttctggtgca agcaacgtcg cgataataca ggggatcaac
     1081 agcgccgcca tcaaccagta catgccctgc cagcccagcc atttatctgc cagccacagg
     1141 gctaagccgc cggaaaccag catccctaaa cggtaaccca gcacgctgat tgccgcgccc
     1201 gcaccacgtt cttctgccgg gagcacatcg gttttccacg catcgaagac aatatcctgg
     1261 gaggcagagc aaaaagcgat caccaccgcc agtgccgcca tccagcggag ctgggtgcct
     1321 ggttcgagaa aacccatcgc cgcaatggcg actaataaca gaatttgcgt ggcgagcagc
     1381 caaccgcgcc gccgcccgaa aaatgggggc gtgtagcggt ccatcagtgg tgaccagagg
     1441 aatttaaaaa cgtaagcctg gcctaccaga gagaagaaac caatggtttt gagatcgata
     1501 ttctcgaccg tcatccaggc ctgtaaggtg ccggacgtca gggcgagcgg tagcccggaa
     1561 gcaaaaccca ggatcagcaa tatggctgaa cgcggctgtt gaaaaatacg taaatattga
     1621 ctggacatgg gtgtctacag gcccggcttg caccgggcca gagggcagaa attaacgcgc
     1681 gttctgcttg atgaattcgt ggatgctggt gtcctgagac atatcagcga tggtatcggt
     1741 cagcacactg ttaaccgcat cggcgatatt tttgttggag gcctggaatg caccttcaac
     1801 gttgtagctg gcacgatagt ttttggtcat tttgttgcca ttctgcgcgg tagcgatgat
     1861 ggcgatatcc gctttggtcg cgatgttgta gcgcacgttg ccctgggaca cgtcagcata
     1921 cagttggcta acgatgattt gcagattaac cgggccattc ggaccaacca tgtatccacg
     1981 ggcggtcatc tgtttttcca gcacttcttg cagcaggaaa cgcagatcgc gggaggcggt
     2041 cagggtaacg atttgattat cgcgggtgac ttttgccagc gcctgatcgg tacgctgatc
     2101 ggcaccatta atgcttacgg tgacgcccat caggcttgga tcctgctgtg gcagtgtaat
     2161 cgtcggggaa acttcgatag ttgttggcgg ttttgcgcat cctgcaagca taaacagagc
     2221 aactaacggg aagaggattt ttttgaacat gttcgggctc tcagagactc ttaagcgtgt
     2281 ttggtaaaaa ttcccgccat cataacattg ccaacggcga ggggaagtgg gtaaggcatg
     2341 taaattcatc atgttgacga aataatcgcc cctggtaaaa gaaacgctga tgcgaggcct
     2401 gtgtttcaat atttaaatca gtaaacttca tacgcttgac ggaaaaacca ggacgaaacc
     2461 taaatatttg ttgttaagct gcaatggaaa cggtaaaagc ggctagtatt taaagggatg
     2521 gatgacatct cagcgttgtc ggaggagata tttcatgatg atacgtgagc ggatagaaga
     2581 aaaattaagg gcggcgttcc aacccgtatt cctcgaagta gtggatgaaa gctatcgtca
     2641 caatgtccca gccggctctg aaagccattt taaagttgtg ctggtcagcg atcgttttac
     2701 gggtgaacgt tttctgaatc gtcatcgaat gatttacagt actttagcgg aggaactctc
     2761 tactaccgtt catgcgctgg ctctgcatac ttacactatt aaggagtggg aagggttgca
     2821 ggacaccgtc tttgcctctc ctccctgtcg tggagcagga agcatcgcgt aaaaacgcat
     2881 ttgcaactgt cggcgctttt ccagtatgtt gctaaagatt ttatgaaaaa cggcctgcgg
     2941 gccgttttgt tttgtctgga ttttgcgctt tttgcccagc attcagacga aaatcgcccg
     3001 ggaattgtga aaaaatacgc gacagcgcgc aataaccgtt ctcgactcat aaaagtgatg
     3061 ccgctataat gccgcgtcct atttgaatgc tttcgggatg attctggtaa cagggaatgt
     3121 gattgattat aagaacatcc cggttccgcg aagccaacaa cctgtgcttg cggggtaaga
     3181 gttgaccgag cactgtgatt ttttgaggta acaagatgca agtttcagtt gaaaccactc
     3241 aaggccttgg ccgccgtgta acgattacta tcgctgctga cagcatcgag accgctgtta
     3301 aaagcgagct ggtcaacgtt gcgaaaaaag tacgtattga cggcttccgc aaaggcaaag
     3361 tgccaatgaa tatcgttgct cagcgttatg gcgcgtctgt acgccaggac gtcctgggcg
     3421 acctgatgag ccgtaacttc attgacgcca tcattaaaga aaaaatcaat ccggctggcg
     3481 caccgaccta tgttccgggc gaatacaagc tgggtgaaga cttcacctac tctgtagagt
     3541 ttgaagttta tccggaagtt gaactgcagg gtctggaagc gatcgaagtt gaaaaaccga
     3601 tcgttgaagt gaccgacgct gacgttgacg gcatgctgga tactctgcgt aaacagcagg
     3661 caacctggaa agaaaaagac ggcgctgttg aagcagaaga ccgtgtgacc atcgacttca
     3721 ccggttctgt agacggcgaa gagtttgaag gcggtaaagc gtctgatttc gtactggcga
     3781 tgggtcaggg tcgtatgatc ccgggctttg aagacggtat caaaggccac aaagctggcg
     3841 aagagttcac catcgacgtg accttcccgg aagagtacca cgcagaaaac ctgaaaggta
     3901 aagcagcgaa attcgctatc aacctgaaga aagttgaaga gcgtgaactg ccggaactga
     3961 ctgaagaatt catcaaacgt ttcggcgttg aagatggttc cgtagaaggt ctgcgcgctg
     4021 aagtgcgtaa aaacatggag cgcgagctga agagcgccat ccgtaaccgc gttaagtctc
     4081 aggcgatcga aggtttggta aaagctaacg acatcgacgt accggctgcg ctgatcgaca
     4141 gcgaaatcga cgttctgcgt cgccaggctg cacagcgttt cggtggcaac gaaaaacaag
     4201 ctctggaact gccgcgcgaa ctgttcgaag aacaggctaa acgccgcgta gttgttggcc
     4261 tgctgctggg cgaagttatc cgcaccaacg agctgaaagc tgacgaagag cgcgtgaaag
     4321 gcctgatcga agagatggct tctgcgtacg aagatccgaa agaagttatc gagttctaca
     4381 gcaaaaacaa agaactgatg gacaacatgc gcaatgttgc tctggaagaa caggctgttg
     4441 aagctgtact ggcgaaagcg aaagtgactg aaaaagaaac cactttcaac gagctgatga
     4501 accagcaggc gtaatttacg tagcataacg cgctaaattc gcacaaaggc ccgtcaccgc
     4561 caggtggtgg gctttttttt gtcatgaatt ttgcatggaa ccgtgcgaaa agcctctttc
     4621 ggtgttagcg taacaacaaa agattgttat gcttgaaata tggtgatgcc gtacccataa
     4681 cacagggact agctgataat ccgtccataa ggttacaatc ggtacagcag gttttttcaa
     4741 ttttatccag gagacggaaa tgtcatacag cggcgaacga gataactttg caccccatat
     4801 ggcgctggtg ccgatggtca ttgaacagac ctcacgaggt gagcgctctt ttgatatcta
     4861 ttctcgtcta cttaaggaac gcgtcatttt tctgactggc caggttgaag atcacatggc
     4921 taacctgatt gtggcgcaga tgctgttcct ggaagcagaa aacccagaaa aagatatcta
     4981 tctgtacatt aactctccag gcggggtgat tactgccggg atgtctatct atgacaccat
     5041 gcagtttatc aagcctgatg tcagcaccat ctgtatgggc caggcggcct cgatgggtgc
     5101 tttcttgctg actgcagggg caaaaggtaa acgtttctgc ctgccgaatt cgcgcgtgat
     5161 gattcaccag ccgttgggcg gctaccaggg ccaggcgacc gatatcgaaa ttcatgcccg
     5221 tgaaattctg aaagttaaag ggcgcatgaa tgaacttatg gcgcttcata cgggtcaatc
     5281 attagaacag attgaacgtg ataccgagcg cgatcgcttc ctttcagccc ctgaagcggt
     5341 ggaatacggt ctggtcgatt cgattctgac ccatcgtaat tgatgccaga ggcgcaactg
     5401 tgccgctata cttatccagg gcggcacaac gctgtaagcg gcttgcgcct gagaatggca
     5461 tttgcgtcgt cgtgtgcggc acaaagaaca aagaagaggt tttgacccat gacagataaa
     5521 cgcaaagatg gctcaggcaa attgctgtat tgctcttttt gcggcaaaag ccagcatgaa
     5581 gtgcgcaagc tgattgccgg tccatccgtg tatatctgcg acgaatgtgt tgatttatgt
     5641 aacgacatca ttcgcgaaga gattaaagaa gttgcaccgc atcgtgaacg cagtgcgcta
     5701 ccgacgccgc atgaaattcg caaccacctg gacgattacg ttatcggtca ggaacaggcg
     5761 aaaaaagtgc tggcggtcgc ggtatacaac cactacaaac gtctgcgcaa cggagatacc
     5821 agcaatggcg tcgagttggg caaaagtaac attctgctga tcggtccgac cggttccggt
     5881 aaaacgctgc tggccgaaac gctggcgcgc ctgctggacg ttccgttcac catggctgac
     5941 gcaaccacgc tgaccgaagc cggttatgtg ggcgaagacg ttgaaaacat cattcagaag
     6001 ctgttgcaga agtgcgatta cgacgtacag aaagcgcagc gcgggattgt ctacatcgat
     6061 gaaatcgaca agatttctcg taagtcagac aacccgtcca ttacccgaga cgtttccggt
     6121 gaaggcgtac agcaggcact gttgaaactg atcgaaggta cggtagctgc tgttccgccg
     6181 caaggtgggc gtaaacatcc gcaacaggaa ttcttgcagg ttgatacctc taagatcctg
     6241 tttatctgtg gcggtgcgtt tgccggtctg gataaagtga tttcccatcg tgtagaaacc
     6301 ggttccggca ttggttttgg cgcgacggta aaagcgaagt ccgacaaagc aagcgaaggc
     6361 gagctgctgg cgcaggttga accggaagat ctgatcaagt ttggtctgat ccctgagttc
     6421 attggtcgtc tgccggttgt cgcaacgttg aatgaactga gcgaagaagc tctgattcag
     6481 atcctcaaag agccgaaaaa cgccctgacc aagcagtatc aggcgctgtt taatctggaa
     6541 ggcgtggatc tggaattccg tgacgaggcg ctggatgcta tcgctaagaa agcgatggcg
     6601 cgtaaaaccg gtgcccgtgg cctgcgttct atcgtagaag ccgcactgct cgataccatg
     6661 tacgatctgc cgtccatgga agatgtcgaa aaagtggtta tcgacgagtc ggtaattgat
     6721 ggtcaaagca aaccgttgct gatttatggt aagccggaag cgcaacaggc atctggtgaa
     6781 taattaacca ttcccataca attagttaac caaaaagggg ggattttatc tcccctttaa
     6841 tttttcctct attctcggcg ttgaatgtgg gggaaacatc cccatatact gacgtacatg
     6901 ttaatagatg gcgtgaagca cagtcgtgtc atctgattac ctggcggaaa ttaaactaag
     6961 agagagctct atgaatcctg agcgttctga acgcattgaa atccccgtat tgccgctgcg
     7021 cgatgtggtg gtttatccgc acatggtcat ccccttattt gtcgggcggg aaaaatccat
     7081 ccgttgtctg gaagcggcga tggaccatga taaaaaaatt atgctggtcg cgcagaaaga
     7141 agcttcaacg gatgagccgg gtgtaaacga tcttttcact gtcgggaccg tggcctctat
     7201 attgcagatg ctgaaactgc ctgacggcac cgtcaaagtg ctggtcgagg ggttacagcg
     7261 cgcgcgtatt tctgcgctct ctgacaatgg cgaacacttt tctgcgaagg cggagtatct
     7321 ggagtcgccg accattgatg agcgggaaca ggaagtgctg gtgcgtactg caatcagcca
     7381 gttcgaaggc tacatcaagc taaacaaaaa aatcccacca gaagtgctga cgtcgctgaa
     7441 tagcatcgac gatccggcgc gtctggcgga taccattgct gcacatatgc cgctgaaact
     7501 ggctgacaaa cagtccgttc tggagatgtc cgacgttaac gaacgtctgg aatatctgat
     7561 ggcaatgatg gaatcggaaa tcgatctgct gcaggttgag aaacgcattc gcaaccgcgt
     7621 taaaaagcag atggagaaat cccagcgtga gtactatctg aacgagcaaa tgaaagctat
     7681 tcagaaagaa ctcggtgaaa tggacgacgc gccggacgaa aacgaagccc tgaagcgcaa
     7741 aatcgacgcg gcgaagatgc cgaaagaggc aaaagagaaa gcggaagcag agctgcagaa
     7801 gctgaaaatg atgtctccga tgtcggcaga agcgaccgta gtgcgtggat atatcgactg
     7861 gatggtacag gtgccgtgga atgcgcgtag caaggtcaaa aaagacctgc gtcaggcgca
     7921 ggaaatcctt gataccgacc attatggtct ggagcgcgtg aaagatcgta tccttgagta
     7981 tcttgcggtt caaagccgtg tcaacaaaat caagggaccg atcctctgcc tggtagggcc
     8041 gccgggggta ggtaaaacct ccctgggtca gtccatcgcc aaagccaccg ggcgtaaata
     8101 tgtccgtatg gcgctgggcg gcgtgcgtga tgaagcggaa atccgtggtc accgccgtac
     8161 ttacatcggt tctatgccgg gtaaactgat ccagaaaatg gcgaaagtgg gcgtgaaaaa
     8221 cccgctgttc ctgctcgatg agatcgacaa aatgtcttct gacatgcgtg gcgatccggc
     8281 ctccgcactg cttgaagtgc tggatccaga gcagaacgta gcgttcagcg accactacct
     8341 ggaagtggat tacgatctca gcgacgtaat gtttgtcgct acgtcgaact ccatgaacat
     8401 tccggcaccg ctgctggatc gtatggaagt gattcgcctc tccggttata ccgaagatga
     8461 aaaactgaac atcgccaaac gtcacctgct gccgaagcag attgaacgta atgcactgaa
     8521 aaaaggtgag ctgaccgtcg acgatagcgc cattatcggc attattcgtt actacacccg
     8581 tgaggcgggc gtgcgtggtc tggagcgtga aatctccaaa ctgtgtcgca aagcggttaa
     8641 gcagttactg ctcgataagt cattaaaaca tatcgaaatt aacggcgata acctgcatga
     8701 ctacctcggt gttcagcgtt tcgactatgg gcgcgcggat aacgaaaacc gtgtcggcca
     8761 ggtaaccggt ctggcgtgga cggaagtggg cggtgacttg ctgaccattg aaaccgcgtg
     8821 cgttccgggg aaaggtaaac tgacctatac cggttcgctc ggcgaagtga tgcaggagtc
     8881 tattcaggcg gcgttaacgg tggttcgtgc gcgtgcggaa aaactgggga tcaaccctga
     8941 tttttacgaa aaacgtgaca tccacgtcca cgtaccggaa ggtgcgacgc cgaaagatgg
     9001 tccgagtgcc ggtattgcca tgtgcaccgc gctggtttct tgcctgaccg gtaacccggt
     9061 tcgtgccgat gtggcaatga ccggtgagat cactctgcgt ggtcaggtac tgccgatcgg
     9121 tggtttgaaa gaaaaactac tggcagcgca tcgcggcggg attaaaacag tgttaattcc
     9181 gttcgaaaat aaacgcgatc tggaagagat tcctgacaac gtaattgccg atctggacat
     9241 tcatcctgtg aagcgcattg aggaagttct gactctggcg ctacaaaatg aaccgtctgg
     9301 catgcaggtt gtgactgcaa aatagtgacc tcgcgcaaaa tgcactaata aaaacagggc
     9361 tggcaggcta attcgggctt gccagccttt ttttgtctcg ctaagttaga tggcggatcg
     9421 ggcttgccct tattaagggg tgttgtaagg ggatggctgg cctgatataa ctgctgcgcg
     9481 ttcgtacctt gaaggattca agtgcgatat aaattataaa gaggaagaga agagtgaata
     9541 aatctcaatt gatcgacaag attgctgcag gggctgatat ctctaaagct gcggctggcc
     9601 gtgcgttaga tgctattatt gcttccgtaa ctgaatctct gaaagaaggg gatgatgtag
     9661 cactggtagg ttttggtact tttgccgtca aagagcgtgc tgcccgtact ggccgcaacc
     9721 cgcagaccgg taaagagatc accatcgctg ctgctaaagt accgagcttc cgtgcaggta
     9781 aagcactgaa agacgcggta aactaagcgt tgtccccagt ggggatgtga cgaagttcaa
     9841 gggcgcatct actgatgtgc cttttttatt tgtattcggt gactttctgc gtcttgtggg
     9901 ctgacaattg cccccgtttc ttgtcacaat aggcctttgc gcgcatcgat acgttgcgtg
     9961 aggtacacag tcatctacag cggagtgttg ttacaccatg atggacagct tacgcacggc
    10021 tgcaaacagt ctcgtgctca agattatttt cggtatcatt atcgtgtcgt tcatattgac
    10081 cggcgtgagt ggttacctga ttggcggagg caataactac gccgcaaaag tgaatgacca
    10141 ggaaatcagc cgtgggcaat tcgagaacgc cttcaacagc gagcgtaatc gcatgcagca
    10201 acagctgggc gatcaatact ccgagctggc agcgaacgaa ggctatatga aaaccctgcg
    10261 tcaacaggtg ctgaatcgtc tgatcgacga ggcgcttctg gatcagtacg ctcgtgagct
    10321 gaaactgggt atcagcgatg agcaggttaa acaggcgatt ttcgcgaccc cagccttcca
    10381 ggttgacggc aaatttgata acagccgcta taacggtatc ctcaaccaga tggggatgac
    10441 cgccgatcag tacgcccagg cgctgcgtaa ccagctcact actcaacagc tgattaacgg
    10501 cgttgccggt accgatttta tgctgaaagg tgaaaccgac gagctggcgg cactggtagc
    10561 tcaacaacgc gtggtgcgtg aagcgactat cgatgttaac gcgctggcgg cgaagcagcc
    10621 tgtgaccgaa caggaaattg ccagctacta cgaacaaaac aaaaacaatt tcatgacgcc
    10681 ggaacaattc cgcgtgagtt acatcaagct ggatgccgcg acgatgcagc aaccggttag
    10741 cgatgcggat atccagagct actacgatca gcatcaggat caattcaccc agccgcagcg
    10801 tacccgttac agcatcatcc agaccaaaac tgaagatgaa gcgaaagcgg tacttgatga
    10861 gctgaataag ggcggtgatt ttgctgcatt agccaaagaa aaatctgccg atattatctc
    10921 tgcccgcaac ggcggtgata tgggttggtt agaagatgcc actatcccgg acgaactgaa
    10981 aaatgctggt ctgaaagaaa aaggccaact gtctggtgtt atcaaatctt cggtcggttt
    11041 cctgattgtg cgtctggacg acattcagcc agcgaaagtg aaatcgttag acgaagtacg
    11101 tgacgacatt gcggcgaaag tgaaacacga aaaagccctc gatgcgtact acgcgctgca
    11161 gcagaaagtg agcgatgcgg caagcaacga caccgagtct ctggccggtg cagagcaagc
    11221 tgccggcgtt aaagccactc agacgggttg gttcagcaaa gataacctgc cggaagagtt
    11281 gaacttcaag ccagttgccg acgctatctt caacggcggt ctggtaggtg aaaacggcgc
    11341 gccgggcatc aactctgaca tcatcaccgt agacggcgac cgcgcattcg tgctgcgcat
    11401 cagcgagcac aaaccggaag cggtgaaacc gttggcagat gttcaggaac aagttaaggc
    11461 actggttcag cacaacaaag ctgaacaaca ggcgaaagtg gatgctgaga aactgctggt
    11521 tgatttgaaa gccggcaaag gtgcggaagc tatgcaggct gccggtctga aatttggcga
    11581 gccgaaaacc ttaagccgtt ccggtcgtga cccgattagc caggctgcgt ttgcactgcc
    11641 actgccagcg aaagacaaac cgagctacgg tatggcgacc gatatgcaag gtaatgtggt
    11701 tctgctggcg ctggatgaag tgaaacaagg ttcaatgccg gaagatcaga aaaaagcgat
    11761 ggtgcagggt atcacccaga acaacgcaca aatcgtcttt gaagctctga tgagtaacct
    11821 gcgtaaagag gcgaaaatca aaattggcga tgcgctggaa cagcaataat cctgaagccg
    11881 cctcgcaaaa aaatgctttg cagctgtaac aagaaaaggt cgctttcgcg gccttttcca
    11941 tttctgaaca ttgccatttg tttactgttt tcactgccgt taaggtgatt ccactgttaa
    12001 caaacaagga gaaaacagta tgaaacacgg aattaaagca ctgctcatta ccctgtccct
    12061 ggcctgtgcc ggaatgtctc atagcgcgct ggcggcagct tctgtggcga aaccgacaac
    12121 agtagaaacc aaagcggaag ctcctgcagc acaaagtaaa gcagcagtac cggcgaaagc
    12181 cagtgacgaa gaaggcaccc gggtcagcat taataatgcc agcgcggaag agttagcccg
    12241 cgcgatgaat ggcgttggcc tgaagaaagc gcaggcgatt gtcagttatc gcgaagagta
    12301 cggtccgttt aaaacggtgg aggatctaaa gcaggtgccg gggatgggca attcgctggt
    12361 ggaacgtaat ctggcggtat taaccctgta attaatttgc atagtggcaa ttttttgcca
    12421 gactgaagag gtcataccag ttatgacctc tgtacttata acagttctta taacaacaac
    12481 gtaaggttat tgcgctatgc aaacacaaat caaagttcgt ggatatcatc tcgacgttta
    12541 ccagcacgta aacaacgccc gctaccttga atttctcgaa gaagcccgct ggcatgggtt
    12601 ggaaaatagc gacagttttc actggatgac ggcccataac atcgccttcg tcgtggtcaa
    12661 tatcaatatt aactatcgcc gcccggcggt attaagcgac ctgttaacca ttaccagtca
    12721 gttgcagcaa ttaaacggta aaagtggcat cttaagccag gtcattacac tggagccgga
    12781 aggacaggtg gtagcggatg cgcttattac gtttgtttgt attgatctta aaacgcagaa
    12841 agcattagct ctggaagggg aattgcgcga aaagctggag cagatggtta agtaaacgtt
    12901 ttgtggtgcc ggatgctcaa gccgcatccg gcgacacccg gaataattac ttcaacccgg
    12961 ttttctgctt catcgctgcc atcaccgtcg gtttatcggc cagataatga ttcaaaccgt
    13021 tggcacgtaa attacacgcc gcacaatgac cgcaaccgtc gcctttaatg ccgttataac
    13081 aggtcagcgt ttcgttacgg actaaatcca gcttgccgta ataatctgcc agcgcccagg
    13141 tttccgcttt atcaatccac atcagcggcg tttcaaaacg aatatctttc gccatgccca
    13201 aactgacggc atggtttagt gctttcacaa actcatcgcg gcaatccggg tagccggaga
    13261 aatccgtttc gcagacgccg gtaattacgg cttctgcttt tacctgatac gcatatattg
    13321 ccgccagtgt caggaacaaa atattacgcc ctgggacaaa cgtattcggg ataccatcgg
    13381 cttcaggttc ataatcaggc accggaatgc tgtcacgcgt caggctgctg accgccagct
    13441 cgttgagcag cgttacatca agcaccttat gcgcgcgtgc ccccagtttc agcgccagtt
    13501 cgcgtgccac gtcgatttct gcgcgatggc gttgaccgta atcgaacgtc acgcaatgga
    13561 cttcatcata ttgttgtaat gcctgcacca gacaggtggt ggaatcctgg cctccactga
    13621 acacaacgac agcacgtttc atagataatc ctgcttgaac aataaaaacg ttatggtaac
    13681 gcctgcgatt aacccggacc agctattcat tgcgacggcg cgggaagcca ggcttcggta
    13741 aattcaaacc agccgcgcgg tgtcagtcgc acaccgttca cgccgggagg ggcgctaatg
    13801 cgatagtgat agttgaacag cggcgtcagc gtcgcatctg ccattaactg gctaaaaacc
    13861 gctttcaggg cattaaatcg gttttcctca tcaggcatta cttgcaccgc gtccagtgtc
    13921 gattgcaaat gtgcatatgc tggagcgtcg aaaacatgtg gccacagtgg atcgcaacgc
    13981 agccattgct ccagggtata ttccggtgct tcgccaatta atctgtcgcc catcatgagg
    14041 tctgcgtgtg ccagtagggt cgtgtcgtcc cagttttttg cgttatgaaa aataattgtg
    14101 agttcacagc cttccgctgc cagtgtcgcc tgtaggcgtt ctgccatggt atgaagttct
    14161 atcggtaggt gataaaccag cgtcaaggtt ttcggtagtt tgacttcatc cggtacttgc
    14221 cattgtggaa tagtccagtc tggcagtaat gcatgactgg cggtgatcag gttttctccg
    14281 acttctaacg tttgtaataa accggattga tgaataatgg agatcacttt tcgcgcctgc
    14341 cagagggaca gtcggggact tttgcgcaac gttaaatagc aaaaacctaa actgatgcca
    14401 ctactgacct ggctgacccg ttgcagctcc tcaggtttgc cgatagtgat ttgcacggga
    14461 tgccgacaac tggttcccaa atctttttcg aaaagcggag gagttatcca gtactcaacc
    14521 gctttaagca gcggatgacg taaatggtaa taatcatggc tttccaggcg caccagttcc
    14581 gctgtgaatt gtgttaagcg aaaaggacca gtgccgatca gggggaattg cggatgcgcc
    14641 agatggctgc aatagctcgc cagccggtgc gcaagccagt aatcggggcg atgtaaaaag
    14701 aaggtcagac actgcggatg ggtgacttca atacgcttca cgctaataaa taattgatcc
    14761 agtgctggca gttgtaacag catcaataac cgctggtgta agtgggaggc ttttactgca
    14821 tcgccgttat gccagtgtaa ggttgagcga agataaaaat cccagcgtaa cccgtcagta
    14881 gaggtttccc agtgatgcgc taaatcgcca atcgggcgct gggtattatt atcgaagcgg
    14941 gtcaggccgg aaaatatctg cccggccaga tgctgctcgg cacggccggg caaaaagccc
    15001 ggttgtagcg gttcgagcgg gcgatagtag ggaatacgca acgtgggcgt atcgttttgc
    15061 cattgtccgc ccataaacgg ctgtaacaga gtgcgcaact cacctggggc cagttgcgcc
    15121 agctccagca catcttgctg ctttccggtt tccagcgcct gttccatcat cgcattgcgt
    15181 agcgattctg gcgtgaccag aaagcgtagt tgaccgcgct ttccgcgtcc tgactgcgcc
    15241 tgccactcca gccatcccgc ctcctgtgcc tgacgcaaca ccgtacgaac atggcgttcg
    15301 ctgcaaaaac agcgttcggc cagttcgctg acggtgacgg tttgcggctt tccggcggaa
    15361 ggttgccaca gacgttgata ctggttaaga cggttgagca gtcgcatata aacccggaac
    15421 aatattattt aactattcac tattacttcc gtatatatca ggtgatactc aatcaccatt
    15481 aaccgtgtca cagagtggag aaagaaatgg ctcgtctggc agcatttgat atggatggca
    15541 ctttattgat gcctgaccat catttaggtg agaaaaccct ctctactttg gcgcgactgc
    15601 gtgaacgcga cattaccctc acttttgcca cggggcgtca tgcgctggag atgcagcata
    15661 ttctcggggc gctatcgctg gatgcatatt tgattactgg caacggaacg cgcgtgcatt
    15721 ctctggaagg tgaactttta catcgtgatg atttacctgc ggatgtcgcg gagctggtgc
    15781 tgtatcagca atgggatacc cgagcaagca tgcatatctt caatgacgat ggttggttta
    15841 ccgggaaaga gatccctgcg ttgttgcagg catttgtcta tagcggtttt cgttatcaga
    15901 taatcgatgt caaaaaaatg ccactcggca gcgtcaccaa gatctgcttc tgtggcgatc
    15961 acgacgatct tacacgcttg cagatccagc tatacgaagc attaggcgag cgtgcacatt
    16021 tatgtttttc cgccacggat tgcctcgaag tgctgccggt gggctgcaat aaaggcgctg
    16081 cattgacggt gctgacccaa catttaggtt tatcgttacg cgattgcatg gcctttggtg
    16141 atgcgatgaa cgatcgcgaa atgttaggca gtgtcggtag cggatttatt atgggcaatg
    16201 cgatgccgca actgcgcgcg gagctcccgc atttaccggt gattggacat tgccgaaatc
    16261 aggctgtctc tcactatttg acgcactggc tggactatcc acatctacct tattcccccg
    16321 aataacgaga tcccttccag caccgggcaa ttgcccggtt ttttttgcgt tgaatttgtc
    16381 attttgtgcc gtggtgctta aaccgcacag aataaattgt cgcgatttca cctttaaaat
    16441 agaattaaaa gagaaaaaaa ttctctgtgg aagggctatg ttagataaaa ttgaccgtaa
    16501 gctgctggcc ttgctacagc aggattgcac cctctctttg caggcactgg ctgaagccgt
    16561 taatctgaca accaccccct gctggaagcg tctgaaacgg ctggaggacg acggtatcct
    16621 tatcggcaaa gtcgccctgc tggatccgga aaaaataggc ctcggcctga ccgcctttgt
    16681 gctgataaaa acgcaacatc acagcagcga atggtattgc cgctttgtca cggtggttac
    16741 cgaaatgcca gaagtgctgg ggttctggcg catggctggt gaatacgatt atctgatgcg
    16801 cgtccaggtt gccgacatga aacgctacga cgagttttat aagcgtctgg taaacagtgt
    16861 accggggctg tcggacgtca cttccagctt cgcgatggaa cagattaaat acaccacttc
    16921 tttacccatc gaataaatat ccagaatcaa gtcaggacac aacgcgtgcg attatttgct
    16981 caattaagct ggtatttccg tcgggaatgg cgtcgctatc tcggggctgt cgccttgctt
    17041 gtcattatcg cgatgctgca actggttccg ccaaaagtgg ttggtattgt tgttgatggc
    17101 gtgacagaac aacactttac taccgggcag atcctgatgt ggatcgccac catggtgctg
    17161 attgccgttg tggtttatct cctgcgttac gtctggcgcg tattgctgtt tggtgcgtct
    17221 tatcaactgg ctgttgaact gcgtgaagat tattaccgcc agctaagccg gcagcatcct
    17281 gagttttacc tgcgtcatcg cacgggtgac ctcatggctc gtgcgaccaa tgacgtcgat
    17341 cgcgtcgtgt ttgccgccgg agaaggggtg ctgacgctgg tggattcact ggtaatgggc
    17401 tgcgctgtgt tgattatgat gtctacgcaa attagctggc agttgacctt atttgccctg
    17461 ttgccgatgc cagtgatggc gatcatgatc aagcgcaacg gcgatgcctt gcatgaacgc
    17521 tttaagctgg cacaggcggc gttttccagt cttaatgacc gcacccagga aagcctcacc
    17581 agtatccgca tgatcaaagc ctttggtctg gaagatcgtc agtcggcgtt atttgccgcg
    17641 gatgccgaag ataccggcaa aaaaaacatg cgggtggcgc gtattgatgc tcgtttcgac
    17701 ccgaccatct atatcgcgat tggtatggcg aacttgctgg cgattggcgg cggtagttgg
    17761 atggtggtgc agggcagttt aacgctgggc cagctcacca gttttatgat gtatttaggt
    17821 ctgatgattt ggccaatgct ggcgctggca tggatgttta acattgtgga acgtggcagc
    17881 gcggcgtata gccgaattcg taccatgctg gccgaagcgc cagtcgttat tgatggtagt
    17941 gataaggtgc cagaaggtcg tggtgaactg gatgtgaata ttcgccagtt tacgtatccg
    18001 cagactgacc atcctgcgct ggaaaacgtc aatttcgccc tgaaacccgg tcagatgctg
    18061 ggtatctgcg ggccgactgg ttccggcaaa agtaccctgt tgtcgctcat tcagcgtcat
    18121 ttcgacgtca gcgagggtga catccgtttt catgatattc ctctgacgaa gttacaactc
    18181 gatagctggc gtagccgtct ggcggtggtc agccagacac cattcctttt ttccgacacc
    18241 gtggcgaaca acatcgcgtt gggttgcccg aatgccaccc agcaagagat tgaacacgta
    18301 gcgcggttag ccagcgtaca tgacgatatt ttgcgtctgc cgcaaggtta cgacacagaa
    18361 gtgggcgagc gcggcgtgat gctttctggc ggacaaaaac agcgtatctc cattgctcga
    18421 gcgttattag tcaacgcgga aatcctcatc cttgatgatg cgctttcagc ggtggacgga
    18481 cgtacagagc accagatcct gcataacctg cgtcagtggg ggcagggaag aacggtaatc
    18541 atcagtgccc atcgcctttc tgcactgacg gaagccagtg aaattattgt gatgcagcac
    18601 gggcatatcg gccagcgtgg caatcatgat gtgctggtac aacaaagcgg ctggtatcgc
    18661 gatatgtatc gctatcaaca actggaggcg gcgctcgacg acgctccgga aattcgcgag
    18721 gaggccatcg atgcgtagtt ttagccaact gtggccgact ctcaagcgcc tgttagcgta
    18781 cggttcaccg tggcgtaaac cgttggggat tgcggtcctg atgatgtggg ttgcggcggc
    18841 ggcagaagtc agtgggccgc tgcttatcag ctattttatc gacaatatgg tagcgaaaaa
    18901 taacctgccg ttgaaagtgg ttgcagggct ggcggcggcg tatgttgggc tgcaactgtt
    18961 tgccgccggg ctacattacg cgcagtcgct gctgtttaat cgggcggcag taggcgtagt
    19021 gcaacagttg cgtaccgacg tgatggatgc tgcgttacgc cagccattaa gcgagtttga
    19081 tacccaaccc gtcgggcagg tgatttcccg cgtcactaat gacactgaag tgatccgcga
    19141 tctctacgtt accgtagtgg caactgtcct gcgtagcgcc gcactggtgg gcgcgatgct
    19201 ggtggcgatg ttcagcctcg actggcgaat ggcgctggtg gcgataatga ttttcccggt
    19261 ggtgctggtg gtaatggtga tatatcagcg ttacagcacg ccgattgtcc gtcgtgtgcg
    19321 cgcttatctg gcggatatca acgacggctt taacgaaatc atcaatggca tgagcgttat
    19381 ccagcagttc cgtcagcagg cgcgatttgg cgaacgtatg ggtgaggcca gtcgttcaca
    19441 ctatatggcg aggatgcaaa ccctgcgcct cgacggtttt ctgctgcgtc cgctgctgag
    19501 tctgttttca tcgctcattc tttgtggctt gttgatgctg tttggcttct ctgccagcgg
    19561 caccattgaa gtgggcgtgt tgtatgcgtt catcagttat ttggggcgac ttaacgaacc
    19621 cttaatcgaa ctgaccacgc agcaggcgat gctgcaacag gctgttgttg ctggtgagcg
    19681 cgtttttgag ctgatggacg gaccgcgcca gcaatatggc aatgatgatc gcccgttgca
    19741 gagcggcacc atcgacgtcg ataacgtgtc atttgcttat cgcgatgaca atctggtgct
    19801 aaagaacatt aatctctctg tgccttcgcg caactttgtg gcgctggtcg ggcataccgg
    19861 cagtggcaaa agcaccctcg ccagtttatt gatgggctat tacccgctaa cggaaggtga
    19921 gattcgcctt gatggtcgtc cattaagtac gctaagtcac agcgcgctgc gccagggcgt
    19981 ggcaatggtg cagcaagatc cggtggtgct ggcggatacc ttcctcgcca acgtgacgct
    20041 gggacgtgat atctctgaag aacgcgtctg gcaggcgctg gaaatcgtgc agctggcgga
    20101 gctggcgcgt agcatgagtg atggtattta cacgccgctt ggcgagcagg ggaataatct
    20161 ctcagtcggg caaaagcaac tgctggcact ggcgcgcgtg ctggtggaga cgccgcaaat
    20221 cctgatcctt gatgaggcaa ccgccagcat tgactccggg actgaacagg cgattcaata
    20281 tgctctggcg gcggtgcgtg aacatactac gcttgtggtg attgctcacc gcttatcgac
    20341 tattgttgat gccgacacca ttctggtgct tcatcgtggg caagctgtgg agcagggcac
    20401 tcaccagcaa ctgctggcgg cccagggacg ctactggcag atgtatcaac tgcaacttgc
    20461 gggcgaagag ctggcagcca gcgtgcgtga agaggaatca ttgagcgcct gaatagcgca
    20521 atatttcatc gttggtgcaa aaatgtaacg cactgtgcac tgtcatagtg cgttttcatt
    20581 ttcaatcttc ttaacttcct gctctctttc tcgtttttca tttctggcac accgcttgca
    20641 ataccttctt cgtgtagcag aaccattacc gaattctgac cggaggggat ctatgaagct
    20701 ggtgaccgtg ataatcaaac cattcaagct ggaagacgtt cgtgaagcgt tatcttccat
    20761 tggtattcag ggcctgaccg tcaccgaagt gaaaggtttc gggcgtcaga aaggacatgc
    20821 cgagctgtac cggggggcgg aatacagcgt caactttctg ccaaaagtaa aaattgatgt
    20881 ggcgattgct gatgaccaac tcgatgaagt gatcgatatc gtcagcaaag cggcttacac
    20941 cggaaaaatt ggcgacggca aaatcttcgt cgctgaattg caacgcgtca ttcgtattcg
    21001 taccggcgaa gccgacgaag cggcgctgta atctctggca cacagcaaca ggaacgaaaa
    21061 atgaagatag caacgataaa aactgggctt acttcactgg cgatgctgcc gggactggta
    21121 atggctgcac ctgcggtggc cgataaagcc gacaatgcgt ttatgatgat ttgtactgcg
    21181 ctggtgctgt ttatgaccgt tccggggatt gccctgtttt atggagggct gattcgtggc
    21241 aaaaacgtgc tgtcgatgct gacacaggtg accgtgacgt ttgcactggt ctgtattctc
    21301 tgggtggttt acggctactc gctggcgttt ggcgaaggca acaacttctt cggcaacatt
    21361 aacgggttga tgctgaaaaa cattgaactg acggcggtga tgggcagcat ttatcagtat
    21421 atccacgtgg cgtttcaggg atcgtttgcc tgcattaccg taggcttgat agttggggcg
    21481 ctggcggaac gaatccgctt ctcagctgtg ttgatttttg tggtggtatg gctgacgctc
    21541 tcttacattc cgattgcgca tatggtctgg ggcggcggtt tgctggcttc tcacggtgca
    21601 ctggattttg cgggtggcac ggtggtgcac attaacgccg caatcgccgg tctggtgggc
    21661 gcgtatctga taggcaaacg cgtgggcttc ggcaaagagg cgtttaaacc gcacaacctg
    21721 ccgatggtct tcaccgggac tgccattctc tatatcggtt ggtttggctt taacgccggg
    21781 tcagcgggca cggcgaatga aatcgcagca ctggcatttg tgaatactgt ggtcgcaacg
    21841 gcggcggcaa ttcttggctg gatcttcggt gaatgggcgc tgcgtggtaa gccttcactg
    21901 ctgggagcgt gttctggcgc gattgccggt ctggtcggcg tgacgccagc ctgtggttac
    21961 attggggttg gcggtgcgtt gattatcggc gtggtagctg gtctggcggg cttgtggggc
    22021 gttaccatgc tcaaacgctt gctgcgggtg gatgatccct gcgatgtctt cggtgtgcac
    22081 ggcgtttgtg gtattgtcgg ctgtatcatg accgggattt ttgccgccag ctcgctgggc
    22141 ggcgtgggct tcgctgaagg tgtgacgatg ggccatcagt tgctggtaca gctggaaagc
    22201 atcgccatta cgattgtctg gtctggtgtg gtggcattta tcggctacaa actggcggat
    22261 ttgacggttg gtctgcgtgt accggaagag caggagcgag aagggctgga cgtcaacagc
    22321 cacggcgaga atgcctataa cgcgtaacaa gcactgcaaa aaacagccgg acggttttca
    22381 cctccggcta tttttttaat tgtgattacg catcacccct tcctgaacgg tcgaggcaac
    22441 cagcacgccg tcctgggtat aaaactcacc gcgcacaaag ccgcgtgcgc tggacgccga
    22501 ggtgctttcc acgctataca gcagccattc attcaaatta aacgggcgat ggaaccacat
    22561 ggaatggtca atggtggcaa tctgaatccc cggttcgaga aaaccgatgc cgtgcggctg
    22621 tagagctacc ggcaggaaat taagatcaga agcataaccg agcagatact gatgaacgcg
    22681 cagatcatcc ggcacgctac catttgcgcg gatccacacc tgacgatgtg gttctgcgac
    22741 gtgacctttc agtgggttat gaaactccac cggacggact tccagcggac gatcgcagat
    22801 gaatttatct ttcagcactg gcggcagcag gtgcgccagc gattgggcga tttgcgtttc
    22861 tgaaggcagg ccatcaggcg ctggcgcgga cggcattgtt ttttgatgtt cgaaacccgc
    22921 ttctggtgcc tggaaagagg cagtcatata aaaaatcggt ttgccgtttt gaatagcagc
    22981 aacccggcgg gcgctgaagc tgttaccgtc acgcagcgtt tcgacatcat aaataatcgg
    23041 cttcttacta tcgccagggc gaagaaagta gctgtgaaaa gaatgtacca gccgctcgtc
    23101 agggacggtc tcttttgcag catacaaggc ctgacccacg acctggccgc caaacacctg
    23161 gcgtaaacct aaatcttcac tctggccgcg aaagagtcct tcctcaattt tttccagatt
    23221 taacaatgtc agtaaatttt ttagcgcctg actcatataa ctctccagta acaaagctgc
    23281 cgcagcaagc caaagtgagt tgagtataac gcaaatttgc cactggtccg atgggtgcaa
    23341 tggtctgaat tacgggctaa ttacaggcag aaatgcgtga tgtgtgccac acttgttgac
    23401 gttactattt tgttaaccac tcttccggcg aggaaagtta gcccgctggt gcattgataa
    23461 taaggagaag tgaatgaaac tcgtgcacat ggccagtggt ttagcggttg cgattgcgtt
    23521 ggcggcttgc gcagataaaa gcgcggatat tcagacgcca gcaccggctg caaatacgtc
    23581 tatttcagca acacaacaac ctgctatcca gcaaccgaat gtctccggta ccgtctggat
    23641 ccgtcagaaa gtcgcactgc cgcctgatgc tgtgctgacc gtgacacttt ctgacgcgtc
    23701 gttagccgat gcaccgtcaa aagtgctggc gcagaaagcg gtgcgtaccg aaggtaaaca
    23761 gtcaccattc agctttgttc tgccatttaa cccggcagat gttcagccga acgcgcgtat
    23821 tctgttgagt gcggcgatta ccgtgaatga caaactggta tttattaccg ataccgttca
    23881 gccggtgatc aaccagggcg gaactaaagc tgacctgaca ttggtgccgg tgcagcaaac
    23941 cgccgtgccg gttcaggcca gcggtggcgc aacgactacc gtaccatcaa cttcaccaac
    24001 ccaggtgaat ccgtcttcgg cagttccagc tccaacgcaa tattaagcga agttaaccct
    24061 ctccgcccgg agagggttag tagttccagc gataaagctg caagtcgatt tgcccgcttc
    24121 ccgacaccat cacaccttct gccagtaatg cctgtcgctg acgctgtaaa tccggtccgg
    24181 tcagtgaaat ggtgccgtgg cgattaacca cccggtgcca tggcaaggtg ctgccttcgg
    24241 ggagacgctt taacacaccg cctacctggc gcgcggcgcg cggcgatccc gccagtttcg
    24301 ccacatcacc gtaagtggtg acatagcctt cgggaatagc ggctacgatt tgccagacgc
    24361 gctggggaaa tgaatcttct ttttccatct tttcttcctg aggtaatttt tcagcataat
    24421 ctggaaaaac gcccgagtga agccgcattg cgcaagaaac cagcatctgg cacgcaatgg
    24481 gttgcaatta gccggggcag cagtgataat gcgcctgcgc gttggttctc aacgctctca
    24541 atgggggctc tgttggttct cccgcaacgc tactctgttt accaggtcag gtccggaagg
    24601 aagcagccaa ggcagatgac gcgtgtgccg ggatgtagct ggcagggccc ccacccattt
    24661 ctgcctccca ccgtttcgtc aaaaaatccc aacatggcta aactttaacc acaactgacg
    24721 tcgcaagaat tgtctggctg cgcagtacgc ttcggaggta tgtctgatga agtatgttga
    24781 tggttttgtt gttgccgttc ctgccgataa aaaggatgcc tatcgggaaa tggccgctaa
    24841 ggccgcgcca ttatttaaag agttcggtgc gcttcgtatt gtcgaatgct gggccagcga
    24901 tgtaccggat ggtaaagtga ccgattttcg tatggcggtg aaagcggaag agaatgaaga
    24961 ggttgtcttt agctggattg aatacccttc aaaagaggtc cgcgacgctg ctaatcaaaa
    25021 gatgatgtcg gacccacgga tgaaagagtt cggcgagtcc atgccgtttg acggcaagcg
    25081 aatgatctat ggtggattcg aatcaatcat cgacgagtag gtagcgtgac gggctgtgcg
    25141 gcgtgccgtc agccctaaaa ttcacaaatg ttgctcggcc cagcgtaaga aatcctcttt
    25201 cggtaatgcc ttgctgtaga gccagccctg accgtagtgc acgccatgct ggcgtagcca
    25261 ctcttcttgt ttactggttt cgattccctc cgcaaccatt ttcagtttca gtgtttttgc
    25321 catttcgatg atatgcggcg tgacattttt atattccagc gcatcaacga aagatttatc
    25381 gatcttcaga atgtcgacat ccagatcctg taaatagctt aaacttgaat accctgtacc
    25441 aaaatcatcg agataaattt catggcccgc ctcccggtag cgagaaatta tcggggcgct
    25501 ggttttcgga tcggcaaact cgcgttcagt gagttcaagc gcgatctgtc tgggattaac
    25561 ctgatagtga ttgatcattt cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt
    25621 ggattcaaga ttgatcgaaa tatgctgctg agaatgctga cgcagccagt cgcccatatc
    25681 ttcaaagacg cttcttataa tcagtagcgt caatggctca gaaaggcccg tttgctgtgc
    25741 cagcggaata aaactatctg gtgacaacca actaccgtct gtctgcggcc agcgcgccag
    25801 tgcctcagca ccgacaattt tgccattggc taaggagaca atcggctgat agtgcacgca
    25861 aatatcacga ttttcgatag catcctgcag ccgatgatgc ggtgactgga tacggcgcaa
    25921 aatacgcagc acaaacatcg ctgccagcag gccaatcacc aacccgagcg gtaaccaaat
    25981 aaagacttgc cgatgccagc ctttctgcaa cattttcgtt gaagcccagg tgatgatcga
    26041 aatattcatc tccggtaagg gcaggatatc gtaaatgatt ccattatttt cgatatgctc
    26101 accgggtgtt ttttgtagcc tggtaataat tccctgagca attttatcgc tgctggttat
    26161 gacaacgtta tgggcattgc caataatggc ggcatcaatt tgccatgagc tatagggaat
    26221 gacatcaata aaggaagcgg ggtcgatcat gacgacataa tgtgccgttc ccatggcgac
    26281 catgtaacga ataatgccta aatcgttatg cgatgttaac cagacacgat aaccatcttt
    26341 cgaaatttta cctggctcgg ggaaggtatc gggcgggctt tcatgctcca gagacgaaca
    26401 ttggggaacg ttgttatcga tataaaccac ttcctgaata tagcgataac tgtaagatac
    26461 ccgacgcatt tccatgagat gggcttcgct acaggcagcg ccttgccatc tttccagctc
    26521 ttgcagcgca tctttccctt gtgtcgccac cttattggcc cgaatagcga cgcgggagga
    26581 atacgtattc agctcttcaa taaatgatgt ttctacctgc tgatgggcca gccagatgct
    26641 taagccaaca ggcagcaata ctgaaagaat cagtactccc gaaataaggc cgaccagatg
    26701 tcgtgttctc accagcatat ccttattaac acagtgtagg gaaagtatat ctgattgtgg
    26761 aaaaaggctg gcaggagata aggaggtaga gctggtattt catgaagtga tgggtggaag
    26821 cgagcgttgt catttgacca ggcgtagagc tttacgcctg gtatgtaccc ctaacttaag
    26881 atgttgattc ggcgcgagcg agggtaaaaa tatcgtaaaa agatagttca cctttacgga
    26941 cgatcatttt ttgcagttgt tctttatgaa catccgcgac acgcggagag ttcaggatgg
    27001 cttcaaccag cgcggcaggt acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc
    27061 ggaaatccag cacgtcgata tcttcatagc ggtaacgtgg gtagacatcg aaaaagaaga
    27121 aaccattaag caggataaat aatacaacca gtagccagac agagcctgaa agcgtttcgg
    27181 attgcaacat caccgccagg gtagcaaaaa aagcaacgta cataccgata aacagccccg
    27241 gatgtttacg gataaaactg atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat
    27301 tcagagactc aatcgtttcg gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg
    27361 atatctgcaa ctttccaggg aacaccctat tttaacgtgg gaatagtaga ggggaagtaa
    27421 cagatttgcc acgattttgc ataacagttg agaaaagctg gttacaattt tttaatgatt
    27481 ctggctggat taccgcccac gacaacgttg tccgggacat cttttgtgac aactgcacct
    27541 gaggctacca cgacgttatc accaatggtc acaccagggt taatgactgc acgtccgcca
    27601 atccagacgt tattaccgat ggtgacgggt ttccccagtt cagcaccgct attacgtgct
    27661 acagggtcga tgggatgtgt tgccgtgtag atatgaacgc ctggtgccaa catacagtta
    27721 tcaccaatgc gaatagggca gacatcaagc atcacgcaat cgaagttggc gaaaaaatta
    27781 ttgccgagaa aaatgttata gccatagtca cagcgaaacg ttggctcaat ataagcctct
    27841 gtcacctgac cgaataaatc agcaagaatt tgctggcgta atgtgtgctc ttccgccagg
    27901 gaatgattgt atcggtgaat aagccgacga gcgcgcaggc gatcgcgaga taacgcctca
    27961 tctgccgagt gatacaactc accagcaatc atcttttctt tttctgtgct cattactgac
    28021 ctccggttgc tgaaaatgcg gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc
    28081 cattacatct gtgtgatgaa gatcacatag ggtatgtctt catggcgaaa aaagtgcaaa
    28141 attcttaata aacagccagt tatagctccg aaagcgagtt atttattagc gaataaattt
    28201 ccatactgag gaagggatct tgtcgtacag tttattcatg gtcaattcgg cgaggcggtg
    28261 atctgcggct gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc
    28321 gataacacgc tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt
    28381 tttcgttaaa ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata
    28441 aactcgcagg attctctttc gtcgcattga caaaacaacg gaatagtcga ttacctgact
    28501 tccgccattt ctgaagatcc tgcatattaa taccataact actgaacaac ataaaggtgt
    28561 catccagata ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt
    28621 aattaagtgc gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg
    28681 aggttgggtc gttaacccag ccatgattgc tttcttcaag gtttgcaagg cagtcatgat
    28741 acagggtttc acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat
    28801 ccataacgcg tccccttctt agcggttgaa ctaacggaca cctttcggga tggaaaaaac
    28861 ttactgacct ggacttgccc tctcgtttgt tagcttaact ataagccact ctttgtaggt
    28921 tttcatcgca ttattacgaa aaattacaat taggaaggaa gcgggacagg actcactttt
    28981 tcatactagc accaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt
    29041 cagaattatc gccagaagaa attggtgaca ctaataccag gattgctctg aatatgacgt
    29101 aataaccgag gaatgaataa agaattaccg caaataatta agaatagcct ctaaatggtt
    29161 atggattatt aaatctatta gcgctactta atataaatta actaacaatc a
//
LOCUS       DADTFG010000053        28940 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.053, whole genome shotgun sequence.
ACCESSION   DADTFG010000053 DADTFG010000000
VERSION     DADTFG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.053"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            106..1689
                     /locus_tag="J1138_002104"
     CDS             106..1689
                     /locus_tag="J1138_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA8453905.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEFEV"
     gene            complement(1757..3610)
                     /gene="asmA"
                     /locus_tag="J1138_002105"
     CDS             complement(1757..3610)
                     /gene="asmA"
                     /locus_tag="J1138_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBA8453906.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRISFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3632..4213)
                     /gene="dcd"
                     /locus_tag="J1138_002106"
     CDS             complement(3632..4213)
                     /gene="dcd"
                     /locus_tag="J1138_002106"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBA8453907.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4305..4946)
                     /gene="udk"
                     /locus_tag="J1138_002107"
     CDS             complement(4305..4946)
                     /gene="udk"
                     /locus_tag="J1138_002107"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBA8453908.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5264..8581
                     /locus_tag="J1138_002108"
     CDS             5264..8581
                     /locus_tag="J1138_002108"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA8453909.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVCHDFFQQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PARLLHLIIPADAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     NAQPLDLLVNRSYFAIN"
     gene            complement(8691..9539)
                     /gene="alkA"
                     /locus_tag="J1138_002109"
     CDS             complement(8691..9539)
                     /gene="alkA"
                     /locus_tag="J1138_002109"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBA8453910.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLHLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANSALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9673..11025
                     /gene="yegD"
                     /locus_tag="J1138_002110"
     CDS             9673..11025
                     /gene="yegD"
                     /locus_tag="J1138_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA8453911.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11038..12978)
                     /gene="yegI"
                     /locus_tag="J1138_002111"
     CDS             complement(11038..12978)
                     /gene="yegI"
                     /locus_tag="J1138_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBA8453912.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALEMDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRPLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILIPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALVALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKIAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(12975..13736)
                     /gene="pphC"
                     /locus_tag="J1138_002112"
     CDS             complement(12975..13736)
                     /gene="pphC"
                     /locus_tag="J1138_002112"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBA8453913.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALAMWLP"
     gene            complement(13733..14392)
                     /locus_tag="J1138_002113"
     CDS             complement(13733..14392)
                     /locus_tag="J1138_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA8453914.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14610..14666)
                     /locus_tag="J1138_002114"
     CDS             complement(14610..14666)
                     /locus_tag="J1138_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBA8453915.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            complement(14938..14994)
                     /locus_tag="J1138_002115"
     CDS             complement(14938..14994)
                     /locus_tag="J1138_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBA8453916.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            15273..16520
                     /gene="mdtA"
                     /locus_tag="J1138_002116"
     CDS             15273..16520
                     /gene="mdtA"
                     /locus_tag="J1138_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBA8453917.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKLVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     TQSATTPEEKATSREYAKKGARS"
     gene            16520..19642
                     /gene="mdtB"
                     /locus_tag="J1138_002117"
     CDS             16520..19642
                     /gene="mdtB"
                     /locus_tag="J1138_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBA8453918.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIADS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19643..22720
                     /gene="mdtC"
                     /locus_tag="J1138_002118"
     CDS             19643..22720
                     /gene="mdtC"
                     /locus_tag="J1138_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBA8453919.1"
                     /translation="MKFFTLFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGAVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            22721..24136
                     /locus_tag="J1138_002119"
     CDS             22721..24136
                     /locus_tag="J1138_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8453920.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGFSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGFGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMASIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24133..25536
                     /gene="baeS"
                     /locus_tag="J1138_002120"
     CDS             24133..25536
                     /gene="baeS"
                     /locus_tag="J1138_002120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBA8453921.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            25533..26255
                     /gene="baeR"
                     /locus_tag="J1138_002121"
     CDS             25533..26255
                     /gene="baeR"
                     /locus_tag="J1138_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBA8453922.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26435..26767
                     /locus_tag="J1138_002122"
     CDS             26435..26767
                     /locus_tag="J1138_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBA8453923.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            26914..28275
                     /locus_tag="J1138_002123"
     CDS             26914..28275
                     /locus_tag="J1138_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBA8453924.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28604..28921)
                     /locus_tag="J1138_002124"
     CDS             complement(28604..28921)
                     /locus_tag="J1138_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453925.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6669 a         7769 c         7641 g         6861 t
ORIGIN      
        1 caaataatat gagataaata gtgctgcaac attgcatttt tgccccgatt tatccatgat
       61 cgaattgtga catttgtcat acaacgaata ggttttgtac ttactatgga atggattgcc
      121 gatccgtcta tctgggccgg gttaatcacg ctgattgtga tcgaactggt cctcggcatt
      181 gataacctgg tctttattgc catcctcgcc gaaaaactac cgcctaagca gcgcgaccgc
      241 gcacgggtta ccgggctgtt gctggcgatg ttaatgcgcc tgttactgct ggcgtcaatc
      301 tcctggctgg tcaccctgac tcaaccgctg ttcagcttcc gctcgtttac ctttagcgcc
      361 cgcgatttaa tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac gatggagctg
      421 aacgaacggc tggaagggaa agacagcaat aaccccaccc aacgcaaagg tgcgaagttc
      481 tggggggtgg tgacacaaat tgtggtactg gacgccatct tctcactcga ctcggtgatt
      541 accgccgtcg ggatggtcga ccatttactg gtcatgatgg ccgccgtggt tatcgctatc
      601 agcctgatgt tgatggccag caagccgtta acccagttcg ttaacagtca cccgacgatc
      661 gttattctct gcttaagctt cctgttgatg attggcttta gcctggtggc ggaaggtttc
      721 ggcttcgtca ttccgaaagg ctacctgtac gctgccattg gtttctcggt gatgatcgag
      781 gcgctcaatc agttggctat ctttaaccgg cgacgttttc tttccgctaa tcagacgctg
      841 cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga agatgcggaa
      901 ctggacgccg aaaccgcgtc catgctggtg gatcatggca accagcagat ctttaatccg
      961 caggaacggc ggatgattga acgggtactt aatcttaacc agcgtaccgt cagcagcatt
     1021 atgacgtcgc gccacgatat tgagcatatc gatctcaacg cgccggaaga cgagatccgc
     1081 cagttactgg agcgaaatca gcatacgcgg ctggtggtta ccgacggtga tgacgcagaa
     1141 gatttgctcg gtgttgttca cgttatcgac ctgctacaac agtctctgcg cggcgaaccg
     1201 ctcaacctgc gggtgttgat tcgccagccg ctggtgttcc cggaaacctt accgttgtta
     1261 cctgccctgg agcagttccg taatgcccgc acacactttg cttttgtggt ggatgagttt
     1321 ggctcggtgg aagggattgt gacattaagt gacgtcactg aaaccattgc cggtaactta
     1381 ccgaatgaag tggaagagat cgacgcccgc catgatattc agaagaatgc ggacggttcc
     1441 tggacggcga atggtcatat gccgctggaa gatctggtgc aatatgtgcc gctgccgctg
     1501 gatgaaaaac gtgaatatca caccattgcc gggctattga tggaatattt gcagcgtatt
     1561 ccaaaacctg gcgaagaagt tcaggtgggg gattatttgc ttaaaacgtt gcaggtggaa
     1621 agccatcgcg tgcagaaggt acagattatt ccgctgcata aggatggcga gatggagttc
     1681 gaggtgtgat ggcatgtagg cctgatgcga cgctttacgc gtcttatcag gcctacagga
     1741 caggtcacct ggtaacctac atcttctcca gcaacttctt cacatctttg ccattgcggg
     1801 aatctttatt ccgctcggcc cagtcgttaa gacggcgttt tgcttcatcc tgtagatgtt
     1861 tacgcagtaa ctgatccact tgcaggctgt aattaagttg ctgccagttg ccataaaccc
     1921 gcagcggtac tggcgtttct ttgaggaaat caatcagttt gctttctccg ttccagccac
     1981 ccacgacccg aatatcaaac tgggtgtcgc aggtttgctc cgccagattc aacatgcctt
     2041 cccctgtcag cgccagcaca ggcgattgac cttgcatgtc gtttaacgtc acgacgccat
     2101 ccttcaacgt caaatcggtg gtaaagcggt caagacgcgt cacgttatca aagttttcag
     2161 cggccttcac atcaccacca ttacgttcta ccgcttgctg aatcatctgc tggaagttca
     2221 tcccttccat gcgtgtatca gtcatttcga catgtgcctg tccttgccag ttgtggcgga
     2281 atgcgtcagc atctatgtca gcaccggaaa aatcaccagc cagtgacatc tttccggtca
     2341 acgaaatcgg atagttaaac gccttcagaa tggtgccaat ctcaacgttt tccagccgtg
     2401 gctggaaact tatccgcgga tttattgatg tcgcgtccag cgtgcccggc agtgaaacct
     2461 gtccaccgtt aagtttgccc tgcagttgag taatttccag caaacccgac ttgttggtca
     2521 tttgcgtggc aacatctgta aaattcattc cgcgccagcg cacgttactg gcctgcaaca
     2581 aaatatcagc cgtaaagcct tgcagtccct gataggccgg ttcatcaata cgcgaagaaa
     2641 tgaccgggcg cggcaacgtt gattggctct gcccttgctg cgcggcaccg ttctcaccat
     2701 tcgctgtttc attaaggggg atgaggttgt caagattcag ttgcgggaat tgcagtctca
     2761 gctgccattc cggtttctct gtcaacgtga cctgtgcttg cccgctcagc gtactgtcat
     2821 tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg tgactcctgc cactggacct
     2881 ggaaactccc ctgcccctga ataccttgtt ttggtaaatc ggcaccctgc aactgccagt
     2941 taatttgttc aatagccgcc attaaatcat gcggataatc agaagcatcc accgtaccgt
     3001 taagggatat cgtgagatcg cgctgatcgc gattaacccg cccggagaac tcaaatgagc
     3061 cacgatgttg gggatcttgt tccatttgca ggcggatatt gcggattgtc acctgctcgt
     3121 catcttcatg ctggaacacc agaacgctgt ccgccacctt aagactggat atatcaaacg
     3181 accatccgcg atcgtctgac agatccggca aggtattgtc gcgcggtgca accggagcgt
     3241 cttcactgcg cacagcttcc gtctgcggcg tcagttggat cactgccccc tttagcatca
     3301 cctgcttaac gctcagttga tgactcagta gtggtaaaag cgccacgtcc agacgcatgt
     3361 tgtcggcgcg aaccagtggc tggcttgcgc cctgggcggt gagagacatt cgcccggaga
     3421 ggatactaag ctgcggccag acatgccaac gcagtggccc gtcgagctgc aattgataac
     3481 cgctacgtgc agcaacttgc ttgaccatat agtcgcggaa atcattcgga ttcaccagca
     3541 acactaacgc agataacccg gccaccagca cgaccaggag tatcatcagc gtcgtcagaa
     3601 atcgtctcat ggtatcctca atgggcctga attagtcttt atcgattcgg ctggctaccg
     3661 cgccctgctg gttgcgatat ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc
     3721 cggagagcgg ctcaaagctc agcgcaccaa ttaacatgcc cggacgcagc gccagcggca
     3781 gcttaccgga gttgtaaaac tccagcacaa tgcagccaga ccagcccgga tcgatacggt
     3841 gcgcggtgac gtgcaccatc agccccagac gcgccagtga agaacgcccg tccagccagc
     3901 ccacaagatc ggctggcagc gtcaccgact ccagcgtcac cgccagcgcc agctctcctg
     3961 ggtgaagata aaacgcctcg ccctcgtcga gcacgatttc atcgctcatc acgcggtcaa
     4021 gcgcggcgct cacttcatct ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac
     4081 ggaaggtacg aaatttattg cccaggcgca catccaccgt cgcgccgtta atacgctcca
     4141 ctggcggacg tgggttgatc gacaaacggc cttcatcaag ccaggcttca atatctcggt
     4201 cacacagacg catggcattt tctcctttcg tgcatcactc ccttaacgcc aattacgtca
     4261 agggcatacc aggttatcac tgaacggtac acaatttatc aagcttattc aaagaactga
     4321 cttattttcg ctttcaatat atcgatcgcg atgcggtttt tcccgccgcg cggcacgata
     4381 atgtccgcat attgtttaga aggctcaatg aattgcagaa acatcgggcg cacggttttt
     4441 tgatattgcg ccatcactga atccattgaa cgcccacgct cgttaacgtc acgcttgatg
     4501 cggcgcatca ggcagatatc cagcggggta tcaacgaaaa tggagaagtt aagttcgtca
     4561 cgcaaacgcg catccgtcag caacaaaatg ccttcgagaa tgatgacctt cttcggctca
     4621 accgtcaccg tttctttcat acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt
     4681 gccgagccgc gtttcaacgc ttgtaaatgc tcaagcagca ggctgtgatc catcgcgctg
     4741 ggatggtcgt agttggtctt aacgcgttct tccatcgaca gatggctttg atctttgtaa
     4801 tagcagtctt cgggaattac gccgatgtgt tcatcaccga cttgctcacg taattcacga
     4861 taaagggtac tggcaataag actcttgccg gaagccgatg cgccagcgat accgataatg
     4921 acgcactgat gagactgatc agtcatatat ttagcgacct gattaacctg gatgttagga
     4981 agggggcgac gaagcgccaa acgcggcaat tatagggatt tcatccgcct gataccagtc
     5041 gaatagcgtt gccgcgcgct cagagttaat ttttgacgaa gaattcccgg tggcaaatta
     5101 cgttgatcag ttttatacaa ggtaaaaaat gttatacgca gttgcgcaaa ttatccgcct
     5161 ttacgtcact ttatgagcaa ttcgcatata aaatgtaaaa cttttgtact agcataaaca
     5221 cagaaacgaa tactggcgac ctggccttgc ggataaagcg gtaatgagca aacaatcaca
     5281 gcatgtatta attgccctgc cccacccgct gcttcacctg gtcagtttag gtttagtctc
     5341 gtttatcttc acccttttct cacttgagct ttcgcagttt ggcacccaac tcgccccact
     5401 gtggttcccg acctccatca tgatggtggc gttttatcgc catgccgggc gcatgtggcc
     5461 gggaattgcg ctgagctgct cgctgggaaa tatcgccgca tccatcctgc ttttttccac
     5521 cagctcgctg aacatgacct ggacgaccat caatattgtt gaagccgtgg tcggggcagt
     5581 gctgctacgt aaattgctgc cgtggtataa ccccttgcaa aatctggctg actggctgcg
     5641 tctggcactc ggcagcgcca ttattccgcc tctgttgggg ggtgttctgg ttgtcctgct
     5701 gacgcccgga gacgatcctc tcagggcatt tttgatatgg gtactgtcag aatccatcgg
     5761 cgctctggca ctggtgccgc tgggattgtt atttaaacca cactatctac tgcgccatcg
     5821 caacccaagg ttgctttttg agtcgctgct cacgttagcc atcacgctga cgttaagctg
     5881 gctttcgatg ctgtatctgc cgtggccttt tactttcatt attgtgctgt tgatgtggag
     5941 cgccgtgcgc ctgccacgaa tggaagcctt tttgatcttc cttaccacag tgatgatggt
     6001 atcgctgatg atggccgcgg atccctccct gcttgctacg ccgcgtacgt acctgatgag
     6061 tcatatgccg tggctaccgt ttttgctgat cctgctgccc gccaacatca tgacgatggt
     6121 gatgtatgcc tttcgtgcgg aacgcaaaca catttccgaa agcgaaaccc gctttcggaa
     6181 cgcgatggaa tattccgcca tcggcatggc attagtgggc accgagggac aatggctgca
     6241 atccaacaaa gcgctctgcc agtttctcgg ttacagtcag gaagagctgc gcggactcac
     6301 ctttcagcaa ctgacctggc cggaggatct caataaagat ctgcaacagg ttgaaaagct
     6361 gataagcggt gaaataaaca cctattcaat ggaaaaacgc tactacaacc gcaatggcga
     6421 tgttgtctgg gcgttgcttg ccgtctcact ggtgcgccac acggatggca cgccgctcta
     6481 ttttatcgct cagattgaag acattaacga gctaaaacgc accgaacagg tgaaccagca
     6541 actgatggag cgcatcacgc tggctaacga agcgggcggg attggcatct gggagtggga
     6601 gctgaagccg aatattttta gctgggataa gcggatgttc gagctgtatg aaattcctcc
     6661 gcatatcaaa ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag atcgccagca
     6721 cgccgaaaaa gtgattcgcg attcattgca atcgcgctct ccctttaaac tggaatttcg
     6781 cattaccgtg aaagacggca ttcgccatat ccgcgccctc gctaaccggg tactgaataa
     6841 agaaggtgaa gtcgaacgcc tgctcggcat taatatggat atgacggagg ttaaacagct
     6901 taacgaggca ttgtttcagg aaaaagagcg cctgcacatt acgctagact ccatcggcga
     6961 agccgtggtc tgtattgata tggcaatgaa aattaccttt atgaatccag tggcggagaa
     7021 gatgagcggc tggacgcagg aagaagcgtt aggtgttccg ctcctgacgg tgttgcatat
     7081 tacttttggc gacaacggac cattaatgga gaacatttat agtgctgaca cctcacgttc
     7141 cgcgattgaa caagatgtgg tgttgcactg ccgaagcggt ggcagttacg acgtgcatta
     7201 cagtattacg ccgttaagta ctctggacgg cagcaatata ggctcggtac tggtgattca
     7261 ggacgtcacc gaatcacgca aaatgctgcg ccagctgagc tacagcgcct cccatgatgc
     7321 actgacgcat ctcgccaatc gcgccagttt tgagaagcaa ctgcgtatcc tgctgcaaac
     7381 ggtaaacagt acacaccagc gacatgccct ggtgtttatc gatcttgatc gctttaaagc
     7441 ggtgaatgac agcgccgggc atgcggcggg cgacgcttta ctgcgcgaac tggcgtcgtt
     7501 gatgctgagt atgctgcgct ccagcgacgt gctggcgcgg ctcggtggtg atgaatttgg
     7561 tctgctactg ccagactgca atgttgaaag cgctcgtttt atcgctacac gcattatcag
     7621 tgccgtgaat gactatcact ttatatggga aggacgtgtg catcgggtag gtgccagtgc
     7681 cgggattacc ttgattgatg acaacaatca tcaggcagct gaagtgatgt cgcaggctga
     7741 tatcgcctgt tatgcctcca aaaatggtgg ccggggccgg gtgacgattt acgaaccgca
     7801 gcaagctgcc gcacatagcg aacgggcggc gatatcgctt gatgaacagt ggcggatgat
     7861 taaagagaat cagttgatga tgatcgccca cggcatcgct tcgccacggc tcccggaagc
     7921 aagtaatttg tggctgattt cacttaagct ctggagttgc gaaagcgaga ttattgatga
     7981 acaaacattt cgtcgtagct tcagcgatcc ggcacttagc catgctcttg accaacgggt
     8041 atgccacgat tttttccagc aggccgcaaa agcggttgcc agtaaaggct taagcatcgc
     8101 ccttcccctt tccgttgctg gtttgagtag cgccacgctg gtgaatgaac tgcttgagca
     8161 gctggaaaat agccctctac cagcacggtt attacatctg attattccgg cagacgcgat
     8221 tttcgatcac gcagaaagcg tgcaaaaact gcggctggcg ggatgtcgga tcgtattcag
     8281 tcaggtgggc cgagatctgc aaatcttcaa ctcgctgaaa gcaaatatgg cagattacct
     8341 gctacttgat ggtgagttat gcgccaacgt gcagggtaat ttgatggatg agatgctgat
     8401 tacgatcatt caggggcacg ctcagcggct cgggatgaag actatcgccg ggccagtcgt
     8461 tttaccctta gtgatggata cgctttcggg catcggcgtc gatctgattt atggtgatgt
     8521 gattgccaat gcccaaccgc tggatttgct ggtgaataga agttatttcg cgattaacta
     8581 agggagcgtt tgcccattgc ctgatgcgac gctaacgcgt cttatcaggc ctacaaatcg
     8641 ctcattcccc aggccggata aggcgctcgc accgcatccg gcgaccaacg tcatgcttcg
     8701 tctggttgcc agccttccgt ataccagata tgcaacagcg cataagaacg ccagggtttc
     8761 cagcgctcgg cataacggcg gatttgtgcc ggcgtcattc ccggaaatcg ctgtttaatc
     8821 agataatcat ccggcagaaa aacatctttc gcctgccagc cacgcaaagc aaaataattt
     8881 gccgtccagc gcccgatacc cggaaaggtt tgcagcgttt tcatcgcttg ttctacgtcg
     8941 cccggtattg tcattggtaa ggtgccctcc agcgccgaat ttgccagatg aatcagcgcc
     9001 tctgcccgtt tcaacggcat acctaacgct tttaatgcct gcgggtcggc tactgccagc
     9061 cgctgaggcg tggggaagca gacataatcc ggaaaatcat ccagccgttc gccataaagc
     9121 tgtgccactc tggaggtcaa ttttgccgcc atcgccacgc tcaccagttg gcctaaaatc
     9181 gcccgcacgc cctgctcaaa agcgtcaaca caaccaggta aatgcaatcc tggccgcgcc
     9241 gcgcctaact tgcccaacgc accgttaaca atctgcgggt tacattgcag atcaaacagg
     9301 cggctcattt tcgccagaca ctcagcggca acaggttcta aacctgcact taaatttatg
     9361 tgcagagtat ggcgggctat atccggaata gcagtcacca cgccgcgata ttcgcctacc
     9421 gccagactac gggcataata actgtccgcg accgtttcca cgccgctcac cgcacgggcg
     9481 gcgagaaatc ccaacatcca cgaccagtca tacggcggct gccagttcag ggtatacatc
     9541 gcatctcctt gttaatccgc tttcagcata aacgttattc agacgttacg ctttgctttc
     9601 atattccggt tgtcgcgacg gcaacatttc gctaaagtca cgccccttct tcaccggcat
     9661 ggggattatt tcgtgtttat tggttttgat tacggtacag caaactgttc agtggcggtc
     9721 atgcgcgacg gtaaaccgca tttgctaaaa atggaaaacg acagcacgct gctgccttca
     9781 atgctttgcg cgccaacgcg tgaagcggta agcgaatggc tgtaccgcca tcatgatgtt
     9841 ccggcagacg acgatgaaac gcaggcgctg ttgcgtcggg cgattcgtta taaccgcgaa
     9901 gaagatatcg atgttacggc gaaaagcgtg cagttcggtc tttcctcact ggcacagtac
     9961 attgatgatc cagaagaagt gtggtttgtg aaatcaccaa aatcgttcct cggtgccagc
    10021 ggcttaaaac cgcagcaggt agcgctgttt gaggatctgg tctgcgcaat gatgttgcac
    10081 attcgccaac aggcgcaggc gcagctgccg gaggcaatta ctcaggcggt gattggtcgc
    10141 ccgatcaact tccaggggct gggcggtgat gaagcaaaca cccaggcgca agggattctg
    10201 gaacgcgcgg cgaagcgtgc cgggtttaag gatgtggtat tccagtacga gccggtcgcg
    10261 gctgggctgg attacgaagc caccttgcag gaagaaaaac gagtgctggt agtggatatc
    10321 ggcggtggta cgactgactg ttcattgctg ctgatgggac cgcaatggcg ttcgcgtctc
    10381 gatcgtgaag ccagcctgct ggggcatagt ggttgccgta ttggtggtaa cgatctggat
    10441 atcgcactgg cgtttaagaa cctgatgccg ttactgggca tgggtggcga aaccgaaaaa
    10501 ggcatcgccc tgccgattct gccgtggtgg aatgcggttg ccatcaacga cgttcctgcg
    10561 caaagtgatt tctacagtag tgctaacggt cgtctgctta acgatctggt acgcgatgcc
    10621 cgtgaaccgg aaaaagtggc cctgttacag aaagtctggc gtcagcgttt aagctatcgc
    10681 ctggtgcgta gcgcagaaga gagcaaaatc gccctttcaa gtgtagcgga aacccgcgcc
    10741 tcactgccgt ttatcagcga tgaactggct acgctgatta gccagcaagg gctggaaagc
    10801 gccctcagcc agccactggc gcggattctg gaacaggtgc aactggcgct ggataacgcc
    10861 caggaaaaac cggacgttat ctacctgacc ggcggtagcg cccgttcgcc gctgattaaa
    10921 aaagcgctgg cagaacagtt gccgggcatt ccgattgcag gcggcgatga ctttggctcc
    10981 gtcaccgccg ggctggcacg ctgggcggaa gtggtgtttc gttaattgaa aaatggctca
    11041 gcaacggctt aaatctgcct gcgcctgagc cagtttttct gccgcctgac gtagcggttc
    11101 catcaacggc atggtccgtg ctggcgcatg aaggcggaat cgctgcaact ctgtcgcacc
    11161 gacagtcagc gccgattcca gccgatgtcg tttggcggct atttttgcca ttaccgcttg
    11221 tctgtctgca ggcgttaccg cttcgttcgg cctgaacaca aaacggcgtt cacagctcgc
    11281 tttccagtcg ataaccgcct gggtcagatg atcaccaaaa ccttttactt gcttaacgct
    11341 ccgtcgggta acatccgctg ctgtttcaat accaaaggac cgtaacgccg ctttacgcgc
    11401 agggccaacg ccgggaatag aggcaacatc aataaaaaat ccctccagaa acttctgctt
    11461 ctgccgttcc cttgcggtgt cgtgaagtgc taccagagcg cgtttctctt cttccggtaa
    11521 cccgagaatt tcgtccttca ttttttcgag catcgcccgt ttggcgataa aaccttccag
    11581 cccgcccaac tgctggatct ggctgactaa atggtcatag tccattttcg cgcgattaaa
    11641 cgcctcacgg cgttgctgga tttctgcttt gtacgctttg cttgtcaggc tgccaataat
    11701 ccagatagcc gccagcacag gaatcaaaat aatataacgc ggttctgcct gaaggccgca
    11761 aagcaacagc gataacgctg acagtgcgat ctcaatcaga atgatgtatt cgcgccgtaa
    11821 caggcctgaa ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg gcagttgcaa
    11881 tgctggcggt gctactgatg ccatcaccat cgcccagact ctcgccagca caaaatcacc
    11941 gccggtggta atgacctctt caccgagatc aataaaatag ataacgcctt gattatccag
    12001 cgcacaccac gggcagtcag tcaaatgagc ggggtaaaca tgcattgccg aaacggtaca
    12061 tttctttaac tgttggcgta gtaaatcaag tgccgctacc cacgcttttg ccgtcggacg
    12121 cccggttgcc acgccacttt ccgtgaatgc ctgctgaaac atggcttcaa catcgcccgg
    12181 taacatcgac agcggaatcg atcgtggcgg cggttttaaa ccacgtcgct gattatctga
    12241 cgcgtaggca taacggaaat gggcaatatc catctccagc gcattacccg catcagagat
    12301 aagcggtaca ccggaataag gatgccgccc accaaacaag acgtgaaata tcagcaacgc
    12361 aaggccaaaa ttatcgtgat tttctgtgcg ttcaaagccg acaaatgatg gcatggtttg
    12421 cagctctggc ggcgtaaaat gcgacacgcc gacttcgcat aaatgcagtg taccattggc
    12481 attaatctga aaggagtcgc tatcaatcaa caccactttg ctgtcgcgac ctaccataaa
    12541 gctgttctgg tttacgtcac ccacgacgtg cccgtgctca tgaacagtag caaaagatga
    12601 agcaatattg cgcgcaacat agagtagaaa atcccacgca caatgagggt aactctggcg
    12661 acgatgtgcc gggctataga tcatatgaat cggttcttta ccagaaactt ttggcatcat
    12721 aaaaccgata acttttccgc ctcgtccacc gtgaagcgtt gcctgcggcc aggcgacata
    12781 attcaacaac tgcgcgtcag ctgttgcagc cataaaggca agtttgtcct gttttaaggc
    12841 gggtggcggc gtgtgataaa tcttggcgac gctatcgaca aactcctcaa tatcataaac
    12901 cgcaccttcg ccgcctttgc ccagttcacg gcctggcgta acgcactcac cagtagcagt
    12961 atataaagtg ggtttcatgg taaccacata gccagtgcca acgttttatc atcatccgta
    13021 cgctcgttta ccgccgggct ggacaaaaac tgctttaaca attcaggtaa taaatcgagt
    13081 tgttcctgcg ttgctgacgc cagtccattg aaaaacgggg taaagaaagg cacatgcgga
    13141 gaattatcca gcatatttaa cgccagtcgc tgaataccat ccgtaaatgc tgcaacttta
    13201 tgcgcacgct cagtgctggt gaaagtttcc agtctggaaa cggcatcttc atcggtaatg
    13261 aagtgcgtca tattggtata ttcacccacc atcggcgtga gcggcagttg caaaccgtgc
    13321 cccaaatcta ccaccacgcc gccatcgcca atctgcataa tcaaggtgcc attagctgat
    13381 gaaatcaatc ccaaaaacgt gcaggcaaaa tcgcgaaccg ctaattcttt ggcttccgcc
    13441 tcggcaaaaa gtcgctgacg aatggtcagc acaatgtccg ttgcgaggac atcattaagc
    13501 cccaattccc cgccctgcac tttttgcgac atataagcca tcgcttcatt gacggcgagc
    13561 atcgcacctt cgcccccctg cgagacgctg cctgcgccat cagcgacgaa taccgacaat
    13621 aatggctgct ggtcatttag ccaggcaacc tgcatctggc aggcatcctg gcaaggcaaa
    13681 tcagcgctaa tatgcgacgt tccaacggct gatgcataga ccagtcgcca gctcacactg
    13741 acgtccagcc ttttggcgct tccagcacaa cttccgttcc cggcgtggag cgggaaaccg
    13801 aacgcagcga actggataac cagctaaaca gttcacggaa ttgtaatccc tgcagagaca
    13861 aaggctgacg gacgctgatt tgggccagcg tcttcatatc cgccccctga acaccgatgg
    13921 taaagaaggc aaacttctta tcttcttccc cctgaaacac tttgttggcg gcagcctgcc
    13981 actcatcggt tggtgcgcca tcggtgatca ggaaaatcca cggacgataa taggaaatac
    14041 cattggcacg atattcacgt ttccgctcct caaccatatc cagggctttg gtaatggcag
    14101 cgcccattgg tgtatcgccc tgggcaaaca ggatgggcgg gaaaaaatta gcggcgctgg
    14161 taaagggttg ttccacatgc accgggccga acgtcacaat gccgagttcc actcttttta
    14221 aggccagcga atcggcaagc agctcatcgc gaaaggtaac caatccggcg ttaagttcat
    14281 taatgggtct accgttcata gaaccagaaa catccagcag caaaatgcag gggcaacgtg
    14341 gttctgggtt actggcaaaa tcgctggtgg caaacgtgat ttgttcgctc ataagaaatc
    14401 ctttcttaga gaattattga ggtccggtat cataccgact gcaataatga aataagagca
    14461 aacgaagaaa ctttcagaat taggcccggc ggcgcagaag attttgctat tcgcctcgca
    14521 agaacgtggg cttacagatt gacaacaagg gttgagtgac ataatttcag gagtgagggt
    14581 agagcggggt ttcccccgcc ctggtagtct tagtatgctg ggtagctaat gactaagagc
    14641 accacgatga tgagtagctt catcatgacc ctttccttat ttaaggcccc ttcctcggga
    14701 ggggctttcc cgtttcagcg tcccgctgaa atcgtcggct tacctccttt cgccctgtat
    14761 gcagtctatc gatgacttgc ggataaaata gtttcaagtg tgattattgc gtaacgactc
    14821 gcaaagaata tttgctggca ggatcgcaga ctgcaaagtc tgcggattga caattggacc
    14881 gtgaaggcat actttcagga atgagggttg agcggggttt cccccgccct ggtagtctta
    14941 gtaagccggg aagcttatga ctaagagcac cacgatgatg atccacttca tcatgaccct
    15001 ttccttattt atggcccctt cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat
    15061 cgtcggctta tctcctttcg ccatgcaagc agtctatcgc taacgcgtag ataaaatagt
    15121 ttcatgtgtt attactggat gcgtgctcgc aaatgtgccc gtcattcaga cgattcccga
    15181 cagtgtttca taattcctcc atttttctcc cttattggct ggctacacta gtatcattcc
    15241 gcgaaacgtt tcaggaagag aaactcttaa cgatgaaagg cagttataaa tcccgttggg
    15301 taatcgtaat cgtggtggtt atcgccgcca tcgccgcatt ctggttctgg caaggccgca
    15361 atgactccca gagtgcagcc ccaggggcga cgaaacaagc gcagcaatcg ccagcgggtg
    15421 gtcgccgtgg tatgcgtgcc ggcccattag ctccggttca ggcggcgacc gcagtagaac
    15481 aggctgttcc gcgttacctc accgggcttg gcaccattac cgccgctaat actgttacgg
    15541 tgcgcagccg cgtggacggt caactgatgg cgttacattt ccaggaaggc cagcaggtca
    15601 aagcaggcga tttactggca gaaattgacc caagccagtt caaagttgca ttagcacaag
    15661 cccagggcca actggcaaaa gataaagcca cgctcgccaa cgcccgccgt gacctggcgc
    15721 gttatcaaca actggcaaaa accaatctcg tatctcgtca ggaactggat gcccaacaag
    15781 cgctggtcag tgaaaccgaa ggtaccatta aggctgatga agcaagcgtc gccagcgcgc
    15841 aattgcaact cgactggagc cgtatcaccg caccagttga tggtcgcgtt ggtctcaagc
    15901 tggttgatgt tggtaaccaa atctccagtg gcgacaccac cgggattgtg gtgatcaccc
    15961 aaacacatcc tatcgatctg gtctttactc tgccggaaag cgatatcgcc accgttgtac
    16021 aggcacaaaa agccggaaaa ccgctggtgg tagaagcctg ggatcgcacc aactctaaga
    16081 agttaagtga aggcacgctg ttaagtctcg ataaccaaat cgatgccact accggtacga
    16141 ttaaagtgaa agcacgcttt aataatcagg atgatgcgct tttccctaat cagtttgtta
    16201 acgctcgcat gttagtcgat accgaacaaa acgccgtggt gatccccacc gccgcactgc
    16261 aaatgggcaa cgaaggccat tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac
    16321 atctggtgac accgggcatt caggacagtc agaaagtggt gatccgcgca ggtatttctg
    16381 cgggcgaccg cgtagtgacg gacggaattg atcgcctgac cgaaggggcg aaagtggaag
    16441 tggtggaaac ccagagcgcc actactccgg aagagaaagc caccagccgc gaatacgcga
    16501 aaaaaggagc acgctcctga tgcaggtgtt acctccgagc agcacaggcg gcccgtcgcg
    16561 cctgtttatt atgcgtcctg tcgccaccac gctgctgatg gtggcgatct tactcgccgg
    16621 gattatcggt tatcgcgccc tgcccgtttc ggcgctgccg gaagtggact atccaaccat
    16681 tcaggtggtc acgctttatc caggtgcgag cccggatgtc atgacctctg ccgttaccgc
    16741 gccgctcgaa cgccagttcg ggcagatgtc cggcctgaaa caaatgtcgt cgcaaagttc
    16801 cggcggtgcg tcagttatca ctttacagtt ccagctaaca ttgccgctgg atgtcgccga
    16861 gcaggaagtg caggccgcga ttaacgctgc cactaacctg ctaccgagcg atctgcctaa
    16921 cccgccggtt tacagcaaag tgaacccggc agatccaccg atcatgacgc tcgctgtcac
    16981 ctcaaccgcc atgccgatga cgcaggtgga agatatggtg gaaacccgcg tcgcgcagaa
    17041 aatctcgcag atttccggcg tagggctggt gacactttcc ggcgggcagc gtccggcggt
    17101 acgcgtaaaa ctcaacgctc aggcaattgc cgctctcggt ctgaccagcg aaaccgtgcg
    17161 caccgccatt accggcgcta acgttaactc ggcaaaaggg agcctcgacg gtccttcccg
    17221 cgcggtcacg ctttccgcga acgaccagat gcaatccgcc gaagagtatc gccagctaat
    17281 catcgcctac cagaacggcg caccgattcg tctgggcgat gtcgcaaccg tagagcaagg
    17341 agcagaaaac agctggctcg gcgcgtgggc gaacaaagaa caggccattg tgatgaatgt
    17401 ccagcgccag cccggtgcta acattatctc caccgccgac agcatccggc agatgctacc
    17461 acagctcacc gagagtctgc cgaaatcggt gaaggtgacg gtactttctg accgtaccac
    17521 caatatccgc gcatcggtcg acgataccca gtttgagctg atgatggcta tcgcgctggt
    17581 agtcatgatt atctacctgt ttttgcgcaa tattccggcg accatcattc ccggtgttgc
    17641 tgtgccgctg tcgttaatcg gcaccttcgc ggtcatggtg tttctcgatt tttcaatcaa
    17701 taacctgacg ttgatggcgt taaccatcgc caccggattt gtggtcgatg acgccatcgt
    17761 ggtgatcgag aacatttccc gctatatcga aaaaggcgaa aaaccgttgg cggcggcgct
    17821 caaaggcgcg ggtgaaatcg gctttaccat tatctcgctg accttctcgc tgattgcggt
    17881 gttgatcccc ctgctgttta tgggcgatat cgtcgggcga ctgttccgcg aatttgctat
    17941 taccctggca gtcgcgattt tgatctcggc ggtggtgtcg ctgaccctga caccgatgat
    18001 gtgcgcgcgg atgctcagcc aggagtcgct gcgtaaacag aaccgcttct cccgtgcctc
    18061 ggagaaaatg ttcgacagga taatcgccgc ctatggtcgt ggactggcga aagtgctgaa
    18121 tcatccgtgg ctgaccttaa gcgtggcgct cagcacgctg ctgcttagcg tgctgctgtg
    18181 ggtgttcatt ccgaagggct tcttcccggt gcaggacaac ggcattattc agggcacttt
    18241 gcaggcaccg caatccagct cctttgccaa tatggcccag cggcaacgcc aggtcgcgga
    18301 cgtgattttg caggatccgg cagtgcaaag tttgacctca tttgttggcg ttgatggcac
    18361 taacccatcg ctgaacagtg cgcgtttaca gatcaatctc aaaccactgg atgaacgaga
    18421 tgaccgggtg caaaaagtca tcgcccgtct gcaaacggcg gtggataaag tgccgggcgt
    18481 cgatctcttc ctgcaaccaa cgcaggacct gactattgat actcaggtca gccgcaccca
    18541 gtaccagttt accttgcagg ccacgtcact ggatgcgctc agtacctggg tgccacagtt
    18601 gatggaaaaa ctccagcagc tgccacagct ttctgatgtc tccagcgact ggcaggacaa
    18661 agggctggtg gcgtatgtca atgtcgacag ggacagcgcc agccgtctgg ggatcagcat
    18721 ggcggatgtc gataacgccc tgtacaacgc gtttggtcag cgactgattt ccactattta
    18781 tactcaggct aaccagtatc gcgtggtgct ggaacacaac accgaaaata ccccaggcct
    18841 cgcggcgctg gataccattc gcctgaccag cagcgacggt ggcgtggtgc cgttaagctc
    18901 aattgccaaa attgagcagc gttttgcgcc gctttccatc aaccatctgg atcagttccc
    18961 ggtaacgact atctccttta acgtgccgga taactattcg ctgggcgatg cggtgcaggc
    19021 gattatggac acagagaaga cgctgaatct gccggtggat atcaccacgc agttccaggg
    19081 cagcaccctc gccttccagt cggcattggg cagcactgtc tggctgattg tcgcggcggt
    19141 ggtggcgatg tatatcgtgc tcggcattct gtacgagagc tttattcacc cgatcaccat
    19201 tctctcgacg ctacccaccg caggggtcgg cgcgctgctg gcgttaatga ttgccgatag
    19261 cgaactggat gtgattgcca ttatcggcat tattttgctg atcggtatcg tgaagaagaa
    19321 cgccatcatg atgatcgact tcgcgctggc tgctgagcgc gagcaaggca tgtcgccgcg
    19381 cgaagctatt taccaggctt gtctgttgcg ttttcgtccg atcctgatga ccactctggc
    19441 ggctctgctt ggtgcgctgc cgctgatgtt gagtaccggg gtcggcgcgg aactgcgtcg
    19501 tccgttaggt atcggcatgg tcggcggtct gattgtcagc caggtgctga cgctgtttac
    19561 cacgccggtg atttatctgc tgttcgaccg tctggcattg tggaccaaaa gccgctttgc
    19621 ccgtcatgaa gaggaggcgt aagtgaagtt ttttaccctc ttcatttacc gcccggtggc
    19681 gacgatttta ctgtcggtcg ccattaccct gtgcggcata ctgggtttcc gtatgctgcc
    19741 agtcgccccg ctgccgcagg tcgattttcc ggtgatcatg gtcagcgcct cgctgcccgg
    19801 tgcgtcacca gaaacaatgg cgtcttccgt tgccacgccg ctggagcgct cacttgggcg
    19861 cattgccgga gtcagcgaaa tgacctccag cagttcgctc ggcagcacgc gcattatttt
    19921 gcagtttgat tttgaccggg atatcaacgg cgcagcgcgc gatgtgcagg cggcgatcaa
    19981 cgccgcgcaa agtctgctac ccagcgggat gcccagccgt ccgacctatc gcaaagcgaa
    20041 cccgtcggat gcgccaatta tgatcctcac gctgacatcc gatacttatt cgcagggtga
    20101 actgtacgat ttcgcctcga cacagctggc tccgacgatt tcgcaaatcg acggtgtcgg
    20161 tgatgtcgat gttggcggca gctcgctgcc cgccgtacgc gtcgggctga atccgcaggc
    20221 gctgtttaat cagggcgtct cgctggacga cgtacgcacc gccatcagca atgccaacgt
    20281 gcgtaaaccg cagggagcgc tggaagatgg tactcaccgc tggcagatcc agactaacga
    20341 tgaactgaaa accgccgctg aatatcagcc actgattatt cattacaaca acggcggcgc
    20401 ggttcgtctg ggcgatgtgg cgacggtgac cgactcagta caggatgtgc gcaacgccgg
    20461 gatgaccaac gccaaaccgg ctattttgct gatgatccgc aagctgccgg aagccaacat
    20521 tatccagacg gtagacagca tccgcgcccg tctgccggaa ttacaatcca ccatcccggc
    20581 agcaattgat ctgcaaattg cccaggatcg ctcccctact atccgcgcgt cgctggaaga
    20641 ggttgagcag acgctgatta tctcggtggc gctggtgatt ctggtggtgt ttttgttcct
    20701 gcgttcgggt cgcgccacca ttattcccgc cgttgcggtg ccggtttcgc tgattggtac
    20761 gtttgcggcg atgtacctgt gtggtttcag tctcaataat ctttcgttaa tggcgctcac
    20821 catcgccact ggctttgtgg tggatgacgc catcgtggtg ctggaaaaca ttgcccgtca
    20881 tctggaagcg gggatgaaac cgctacaggc cgcgctacaa ggtactcgcg aagtcggctt
    20941 tacggtgctg tcgatgagtc tgtcactggt ggcggtgttc ctgccgctgc tgttgatggg
    21001 cggattgccg ggtcgcctgt tacgcgagtt tgccgtgacg ctttctgtcg ccattggtat
    21061 ttcgctgctg gtttccctga cgttaacgcc gatgatgtgc ggctggatgc taaaagccag
    21121 caagccgcgc gagcaaaagc gactgcgtgg ctttggtcgc atgttggttg ctctgcaaca
    21181 aggctacggc aagtcgctaa aatgggtgct caatcatacc cgtctggtgg gcgcggtgct
    21241 gcttggcacc attgcgctga atatctggct gtatatctcg atcccgaaaa ccttcttccc
    21301 ggagcaggac accggcgtac tgatgggcgg gatacaggct gaccagagta tttcgtttca
    21361 ggcgatgcgc ggtaagttgc aggacttcat gaaaattatc cgtgacgatc cggcggtgga
    21421 taatgtcacc ggctttaccg gcggttcacg ggtgaacagt ggaatgatgt ttatcaccct
    21481 taaaccacgc ggcgagcgga gcgaaacggc ccaacagatt atcgatcgct tgcgtaaaaa
    21541 actggcgaaa gaaccgggag cgaatctgtt cctgatggcg gtacaggata ttcgcgttgg
    21601 cggacgtcag gctaacgcca gttaccagta cacgttgtta tccgacgacc tggcggcact
    21661 gcgcgaatgg gagccaaaaa tccgcaaaaa actggcgaca ttgccagagc tggctgatgt
    21721 taactccgat caggaagata atggcgcgga gatgaacctt atttacgatc gcgacactat
    21781 ggcgcgattg ggaattgacg tgcaagccgc caacagcctg ttaaataacg ccttcggtca
    21841 gcggcaaatc tcgaccattt accagccgat gaaccagtac aaagtggtga tggaagttga
    21901 tccgcgctat acccaggaca tcagtgcact ggaaaaaatg ttcgttatca ataacgaagg
    21961 aaaagcgatc ccgctgtcgt atttcgctaa atggcaaccg gcgaacgctc cactgtcggt
    22021 gaatcatcag ggattatcgg cggcctcgac catttcgttc aacctgccga ccgggaaatc
    22081 gctctcggac gccagtgcgg cgatcgatcg cgcaatgacc cagcttggtg tgccttcgac
    22141 ggtgcgcggc agttttgccg gaacagcgca agtgttccag gagacgatga actcacaggt
    22201 gatcctgatt atcgccgcca tcgccacggt atatatcgta ctgggtatcc tttacgagag
    22261 ttatgtgcat ccactgacga ttctctccac cctaccctcg gcgggcgtcg gagcgctgtt
    22321 ggcgctggag ctgttcaatg ccccgttcag cctaattgcg ctgataggga tcatgttatt
    22381 aatcggcatc gtgaagaaaa acgccattat gatggtcgat tttgcgcttg aagcccaacg
    22441 gcacggtaac ctgacgccgc aggaagctat tttccaggcc tgtctgctgc gttttcgccc
    22501 gattatgatg actaccctgg cggcgctgtt tggtgcgctg ccgctggtat tgtcgggcgg
    22561 tgacggctcg gagttgcggc aaccattggg gatcaccatt gtcggcggtc tggtcatgag
    22621 ccagcttctg acgctgtata ccacgccggt ggtgtatctc tttttcgacc gtctgcgact
    22681 gcgtttttcg cgtaaaccta aacaagcggt aaccgagtaa atgacagatc ttcccgacag
    22741 cacccgctgg caattgtgga ttgtggcttt cggcttcttt atgcagtcgc tggacaccac
    22801 catcgttaac accgcgcttc cctcaatggc gcaaagcctc ggggaaagtc cgttgcatat
    22861 gcacatggtc attgtctctt atgtactgac cgtggcggtg atgctgcccg ccagcggctg
    22921 gctggcggac aaagtcggcg tgcgcaatat tttctttacc gccatcgtgc tgtttactct
    22981 cggttcactg ttttgcgcgc tttccggcac gctgaacgaa ctgttgctgg cacgcgcgtt
    23041 acagggcgtt ggcggcgcga tgatggtgcc ggtcggcagg ttgacggtga tgaaaatcgt
    23101 accgcgcgag caatatatgg cggcgatgac ctttgtcacg ctgcccggtc agatcgggcc
    23161 gctgcttggc ccggcgctcg gcggtctgct ggtggagtac gcatcgtggc actggatctt
    23221 tttgatcaac attccggtgg ggattattgg cgcgatcacc acactgatgt taatgccgaa
    23281 ctacaccatg cagacgcggc gctttgatct ctccggcttt ctgttgctgg cggttggcat
    23341 ggcggtctta accctggcgc tggacggcag taaaggtaca ggtttctcgc cgctggcgat
    23401 tgctggcctg gtcgcagttg gcgtggtagc actggtgctt tatctgctgc acgcccaaaa
    23461 taacaaccgc gccctgttca gtctgaaact gttccgcact cgtacctttt cgctgggcct
    23521 ggcggggagc tttgccggac gtattggcag tggcatgttg ccctttatga caccggtttt
    23581 cctgcaaatt ggtttcggtt tctcgccgtt tcatgccggg ctgatgatga tcccgatggt
    23641 actcggcagc atgggaatga agcgaattgt ggtacaggta gtgaatcgct ttggttatcg
    23701 tcgggtactg gtggcgacca cgctgggcct gtcgctggtc accctgttgt ttatgactac
    23761 cgctctgctg ggctggtact acgttttgcc gttcgtcctg tttttacaag gaatggtcaa
    23821 ctcgacgcgt ttctcctcca tgaacaccct gacgctgaaa gatctcccgg acaacctggc
    23881 gagcagcggc aacagcctgc tgtcaatgat tatgcaattg tcgatgagta ttggcgtcac
    23941 catcgccggg ctgttgctgg gactttttgg ttcgcagcat gtcagcgtcg acagcggcac
    24001 cacacaaacc gtctttatgt acacctggct tagcatggcg tctatcatcg cccttccggc
    24061 gttcatcttt gccagagtgc cgaacgatac gcatcaaaat gtggctattt cacggcgaaa
    24121 aaggagcgcg caatgaagtt ctggcgaccc ggtattaccg gcaaactgtt tctggcgatt
    24181 ttcgccacct gcattgtctt attgatcacg atgcactggg cggtacgtat cagttttgag
    24241 cgcggcttta tcgattacat caagcatggt aatgaacaac ggctgcaaat gctcggcgat
    24301 gcgcttggtg agcagtacgc ccagcacggg aactggcgct tcctgcgtaa taacgatcgc
    24361 tttgtatttc agatcctacg ttcactggag catgataaca acgaagataa gcccggcccc
    24421 ggtatgccac cacacggctg gcgcacgcaa ttttgggtgg ttgatcaaaa taataaagtg
    24481 ctggttggcc cgcgagcacc ggttccaccc gacggcacac ggcggcccat tatggtcaat
    24541 ggtgcggaag ttggcgcggt gatcgcctcc cctgttgaac gactgacccg taatactgat
    24601 atcaattttg acagacaaca gcggcaaacc agttggctga ttgtggcttt atctacctta
    24661 ttagcggcgc tggcgacatt cccactggcg cgcggtttgc tggctccggt aaaacgactg
    24721 gtggatggca cgcacaaact ggcagcgggt gatttcacta ctcgcgtagc ccccaccagc
    24781 gaagatgagt tgggcagact ggcagaggat ttcaatcaac tcgccagcac actggagaaa
    24841 aaccagcaga tgcgccgcga ttttatggcc gatatttctc atgaactgcg cacgccttta
    24901 gcagtactgc gcggcgaact ggaagccatt caggatggtg tgcgtaaatt cacgccggag
    24961 acgctggctt ctttacaggc agaagtcgga acactgacaa aactggttga cgatctccat
    25021 caattgtcta tgtctgatga aggcgctctc gcctatcaaa aagcaccggt agatttgatc
    25081 ccactgctgg aagtggcggg aggcgcattt cgcgaacgat tcgccagccg tggcctgaaa
    25141 ttgcaatttt ccctgccaga cagtattacc gtatttggcg atcgcgatcg tttaatgcag
    25201 ttgttcaata acttactgga aaacagcctg cgctacactg acagcggcgg cagcctgaaa
    25261 atctctgccg aacagcacga caaaacggtg cgcctgacct ttgccgacag tgcgccgggt
    25321 gtcagtgacg atcagctaca aaaattgttt gaacgttttt atcgcaccga aggttcccgc
    25381 aaccgcgcca gcggtggttc cgggctgggg ctggcgattt gcctgaacat tgttgaagca
    25441 cataacggcc gcattattgc cgcccattcg ccttttggcg ggcttagcat tacagtagag
    25501 ttaccgctgg aacgggattt acagagagaa gtatgaccga gttaccaatc gacgaaaaca
    25561 caccgcgtat tttgatcgtg gaagatgaac cgaagctggg gcagttgctc attgattatc
    25621 tgcgtgctgc gagctatgcg ccaacgttga ttagccacgg cgatcaggta ctgccatatg
    25681 tgcgccagac accaccggat ctgatcctgt tggatctgat gctccctggc accgatggcc
    25741 tgacgctgtg ccgggaaatt cgtcgttttt ctgacattcc gatcgtgatg gtgacggcaa
    25801 aaatcgaaga gatcgaccgc ctgctggggt tggagattgg cgcagatgac tatatctgca
    25861 agccgtacag cccacgggaa gtggtagcgc gcgtcaaaac cattttgcgc cgttgcaaac
    25921 cgcagcgtga gttgcagcaa caggatgctg aaagcccgtt gattatcgac gaaggtcgtt
    25981 ttcaggcttc ctggcgcggt aaaatgcttg acctgacgcc tgcagaattt cgtctgctga
    26041 aaacgctctc tcatgaacca ggaaaagtgt tctcccgcga gcagttgctg aaccatcttt
    26101 atgacgacta ccgcgtggta accgaccgta ccatcgacag ccacattaaa aacctgcgcc
    26161 gcaagctgga atctctcgac gccgaacagt catttatccg cgccgtttac ggcgtcggtt
    26221 accgctggga agccgacgcc tgccgcatcg tttaacattt ttacctgccg gagatggcct
    26281 tcagaccaga tccggcaaca ttatcccacg catggtcagc aaactgacat tatctctccg
    26341 ccctggcagg caccagaaaa tcgctcatac tttaattggc aaacagtacc tttagatgct
    26401 gttttcgata cgcaatttca atcaaggagt cattatggct ggttggtttg aactcagtaa
    26461 gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc aatggtgaga ctatcctcac
    26521 cagcgagctg tatacctcaa aagcctctgc ggaaaagggc atcgcgtcgg tgcgtagcaa
    26581 cagcccgcaa gaagaacgct atgagaaaaa aacggcaagc aatggcaaat tctatttcaa
    26641 tctgaaagcc gccaatcatc aaattatcgg ctccagccag atgtatgcca ccgcgcaatc
    26701 tcgtgaaacc ggaatagcct ccgttaaagc caatggcaca agccagacgg tgaaagacaa
    26761 tacgtaatta cgatgccggg cgtaggttgt tggcgtccgg cgctttacca gttaccccca
    26821 cagcgctaca atgcccgccc ttaaagtggg ggcactcccc taaccgcttc atcaggtgaa
    26881 gcggctctga cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg
    26941 gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg
    27001 ggccagccgc gttactccct gcgtgtgcgc aacaacgaat tcaaccacga aaatcttcag
    27061 ctcggcatca atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca
    27121 ccgcacaacg ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg
    27181 ccggatgcgc tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gcacttcccg
    27241 gaaatgccaa tccacctctc ggtacaggct aacgccgtaa actgggcgac ggtgaaattc
    27301 tggcagcaaa tgggtctgac ccgcgtgatc ctctctcgcg aactgtcact ggaagagatt
    27361 gaagagatcc gcaatcaggt gccggatatg gagatcgaaa tcttcgttca cggcgcgctg
    27421 tgcatggcct actccggtcg ctgcctgctc tctggctata tcaacaagcg cgacccgaac
    27481 cagggcacct gcaccaacgc ctgccgctgg gagtataacg tccaggaagg gaaagaagat
    27541 gacgtcggca acatcgtaca caagtacgag ccgattccgg tgcaaaatgt tgagccgacg
    27601 ctgggtatcg gcgcgccaac cgacaaagtg tttatgatcg aagaagccca gcgtccgggc
    27661 gagtatatga ccgcgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg
    27721 cgcgccatcg cccatgttga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa
    27781 ggccgtacca aatctttcta ctattgcgca cgcaccgcgc aggtttaccg taaagctatc
    27841 gatgacgccg ctgcgggcaa accgttcgat accagcctgc tggaaactct ggaaggtctg
    27901 gcgcatcgtg gctataccga aggtttcctg cgtcgtcata cccacgacga ttatcagaac
    27961 tacgaatacg gttattcggt ttctgaccgt cagcagtttg ttggtgagtt taccggtgag
    28021 cgcaaggggg agctcgcggc ggtagcggtg aaaaataaat tctccgttgg cgacagcctt
    28081 gagctgatga cgccgcaagg caacattaac tttacccttg agcacatgga aaacgccaaa
    28141 ggtgaagcaa tgccggtcgc accaggcgat ggttatactg tgtggctccc ggtgccgcag
    28201 gatcttgagc taaattacgc gctgctgatg cgtaatttct ccggggaaac cacgcgtaac
    28261 ccccacggta agtgattaat ttcgattatt tttcccggat ggaaaattct tagaaaccga
    28321 tcacatacag ctgcatttat taaggttatc atccgtttcg ctgaaaaaca taacccataa
    28381 aatgctagct gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta
    28441 ttttttatgt ataagccgta tcctggtaac cgtcttccat tgaccacatc gaaagaatct
    28501 cccttcatag cacgatgcct ttcacttatt ggcatcgtgc tcgcacaggt tccggttacg
    28561 cacagccaga acgcgcatgt ttgatgctta ccaaaaaata ttctcactct ccacatttga
    28621 atgtcagacg agcgacgcca tgtaatcctg caccttctgt cttcaggtca actatctgca
    28681 tttttttgcc ctgagtaaca cagaaatgaa ctgcatcatt ttttactata ttttctgcac
    28741 cagatattct acccctggct aaagaagctt cggcttcggt gtagtattgg ttatcgagtt
    28801 tacgctgaat attactttta tatgcaagac caaatttacc gatacttgtc tcatcattat
    28861 gtacagcaca cccagacata agaaaaacac taattaatga tatagcagct atctttttca
    28921 tctcaccttc ccccattaaa
//
LOCUS       DADTFG010000054        28737 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.054, whole genome shotgun sequence.
ACCESSION   DADTFG010000054 DADTFG010000000
VERSION     DADTFG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.054"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(27..1151)
                     /locus_tag="J1138_002125"
     CDS             complement(27..1151)
                     /locus_tag="J1138_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA8453926.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTYAIEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKKYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGVCLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVAQMLTSLALKYNESLTPSC"
     gene            1284..2837
                     /locus_tag="J1138_002126"
     CDS             1284..2837
                     /locus_tag="J1138_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA8453927.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDQQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3219..4109
                     /gene="metF"
                     /locus_tag="J1138_002127"
     CDS             3219..4109
                     /gene="metF"
                     /locus_tag="J1138_002127"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBA8453928.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ACDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4438..6618
                     /gene="katG"
                     /locus_tag="J1138_002128"
     CDS             4438..6618
                     /gene="katG"
                     /locus_tag="J1138_002128"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBA8453929.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6712..7617
                     /gene="yijE"
                     /locus_tag="J1138_002129"
     CDS             6712..7617
                     /gene="yijE"
                     /locus_tag="J1138_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBA8453930.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARNPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7644..8261)
                     /locus_tag="J1138_002130"
                     /pseudo
     CDS             complement(7644..8261)
                     /locus_tag="J1138_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(8537..9640)
                     /gene="gldA"
                     /locus_tag="J1138_002131"
     CDS             complement(8537..9640)
                     /gene="gldA"
                     /locus_tag="J1138_002131"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBA8453931.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9651..10313)
                     /gene="fsa"
                     /locus_tag="J1138_002132"
     CDS             complement(9651..10313)
                     /gene="fsa"
                     /locus_tag="J1138_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBA8453932.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWELLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFENDWNAAFGTT
                     HL"
     gene            complement(10325..12826)
                     /gene="ptsP"
                     /locus_tag="J1138_002133"
     CDS             complement(10325..12826)
                     /gene="ptsP"
                     /locus_tag="J1138_002133"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA8453933.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     AHHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13135..14214
                     /locus_tag="J1138_002134"
     CDS             13135..14214
                     /locus_tag="J1138_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBA8453934.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            14229..14549
                     /locus_tag="J1138_002135"
     CDS             14229..14549
                     /locus_tag="J1138_002135"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBA8453935.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14600..16897
                     /locus_tag="J1138_002136"
     CDS             14600..16897
                     /locus_tag="J1138_002136"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBA8453936.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16863..17741
                     /locus_tag="J1138_002137"
     CDS             16863..17741
                     /locus_tag="J1138_002137"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBA8453937.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPDS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17743..18084
                     /locus_tag="J1138_002138"
     CDS             17743..18084
                     /locus_tag="J1138_002138"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBA8453938.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18071..18922)
                     /locus_tag="J1138_002139"
     CDS             complement(18071..18922)
                     /locus_tag="J1138_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8453939.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQSPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVTSLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19148..20881)
                     /gene="cptA"
                     /locus_tag="J1138_002140"
     CDS             complement(19148..20881)
                     /gene="cptA"
                     /locus_tag="J1138_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBA8453940.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21064..23715)
                     /gene="ppc"
                     /locus_tag="J1138_002141"
     CDS             complement(21064..23715)
                     /gene="ppc"
                     /locus_tag="J1138_002141"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBA8453941.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24017..25168)
                     /gene="argE"
                     /locus_tag="J1138_002142"
     CDS             complement(24017..25168)
                     /gene="argE"
                     /locus_tag="J1138_002142"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBA8453942.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25322..26326
                     /gene="argC"
                     /locus_tag="J1138_002143"
     CDS             25322..26326
                     /gene="argC"
                     /locus_tag="J1138_002143"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBA8453943.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26334..27110
                     /gene="argB"
                     /locus_tag="J1138_002144"
     CDS             26334..27110
                     /gene="argB"
                     /locus_tag="J1138_002144"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBA8453944.1"
                     /translation="MMNPLIIKLGGVLLDSEEALDRLFSALVNYRESHQRQLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTNEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27171..28544
                     /gene="argH"
                     /locus_tag="J1138_002145"
     CDS             27171..28544
                     /gene="argH"
                     /locus_tag="J1138_002145"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBA8453945.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
BASE COUNT         6654 a         7204 c         7907 g         6972 t
ORIGIN      
        1 ttatggtcgt ttatcgagtg tattgactaa caagatggag tgagcgattc attgtatttt
       61 aacgccagtg aggtcagcat ttgcgctacg cgacctgcgg ataataacgc gctttgggta
      121 aagtcgtgtt ggtctgtcag ccatgtgata aaacaacgac tggttgccag agaaacattt
      181 gcgccccttt ctaagcagac tcccgtgagt aggcagacca tcttgcagtt attcccctgc
      241 tcccagcgct gccactgatt ttgccaccaa ccatggtatg tcgttccctt aaaatgccag
      301 ggcgtatcag cgttactgtc actgatttct tcttctacga accaaagttc accttgttgt
      361 tggcggaact gatctgccca ggctgtaata attgcgcctc ttagaaaaca ttcgtgtgaa
      421 ataatatgcg ttttgacatc tggatagcgg attttcagta ttctgaaatg cccgtgtgcc
      481 gtgataaccg ggcgtagtat ggctccctta ccgttgcgct tgagtacagc actgccatac
      541 tgaatttcat cgaactggct ccggcgcaag aattgcgctt cacgcatatc taccttctgc
      601 acgatatcgc gatggtgcat agttggttct gtgttgccct tgtaactgta ccgcagactg
      661 tctcctgtct gatgttgcgt ataattcgtg gagagatgaa gaatgttgcc acgagtggcg
      721 tcaagactga ctaaaacata cagtttttgt actgcgctgg ccccttgaaa gggtatgaac
      781 agttgaacgg tttcaatggc ctggggatgc gtaatttctt tctgatattt tttaggcgtc
      841 cagacttttt tgcagttacg gcattgaatg cgttgcgaac cctgtggatt gtgaccataa
      901 caaatcatgt ctgtgccata acataccggg cagaaatggc ctttctcaat tgcataagta
      961 gacaagtgaa ctgacaacca gtcacgaaat tgttgctcgt caaacaacgg cgggtaactg
     1021 ccacatgccc gacagtgcag tgccggataa cccagtttat agtctggcca actgtaatct
     1081 tccgatgagg ccaatcccaa atttcggcaa ccgaacgttt tacaggcatt aatcgtaaac
     1141 agagtcgaca tatatcacca acgatttcac aaggtcgttg attttgtccg gcaactatcc
     1201 cctgcggatg tgattactct cacataaata agtctttcag aacgtaatgt agcaatcctt
     1261 tatctcactg aaagggattg tttatgaaaa ttaaaatact tgctgcgggt atcgcattaa
     1321 cactgccgtt ctgggcttgc gctaaagatg tcactattat ttataccaac gacctccatg
     1381 cgcatgtaga accttataaa gtgccgtgga tcgctgacgg taaacgggat attggcggct
     1441 gggcaaatat cactacgctg gttaagcaag aaaaagcaaa aaataaagcg acatggtttt
     1501 ttgatgcggg cgactatttt accgggccat atatcagcag cctgaccaaa ggtaaggcta
     1561 ttatcgatat tatgaatact atgccgtttg atgccgtcac aataggcaac catgaatttg
     1621 accacggctg ggacaacaca ttattacagt tgagccaggc aaaattccct attgtgcagg
     1681 gcaatatttt ttatcagaac agcagtaaat cattctggga taagccctat accatcatcg
     1741 aaaaagacgg tgtgaaaata ggcgttattg gtttgcacgg agtatttgcc tttaatgata
     1801 cggtatctgc tgccacgcgc gtgggtattg aagcgcgcga tgaaattaaa tggctacaac
     1861 gttatatcga tgaactcaaa ggcaaggttg atttgaccgt cgccctgatc cacgaaggtg
     1921 ttccagcccg ccagtccagt atgggaggca cagatgtgcg tcgcgcgttg gataaagata
     1981 ttcagacggc aagtcaggtg aaagggctgg atattttgat caccggacat gcacatgtgg
     2041 gtacgccgga accgattaaa gtcggcaata cgctcatcct ctccactgac agcggcggga
     2101 ttgatgtggg caaactggtt ctcgactaca aagagaagcc gcataatttt acggtgaaaa
     2161 acttcgagct taaaaccatt tacgctgacg agtggaagcc cgatcagcaa acgaaacagg
     2221 tgatcgacgg ctggaataaa aagcttgatg aagtggtgca acaaacggtg gcgcaatcgc
     2281 cggttgaatt aaaacgtgcc tatggtgaat cggcttccct cgggaacctg gcggctgacg
     2341 ctttactggc agcagcgggt aaaaataccc aactggcgtt aacaaactct ggtggtatcc
     2401 gcaatgagat ccccgcaggg gcgatcacga tgggcggcgt tatcagtacg ttcccgttcc
     2461 ccaacgaact ggtgacgatg gaactcacgg gtaaacaatt acgcagtctg atggaacacg
     2521 gcgcaagttt aagcaatggc gttttacagg tatcgaaagg cctggaaatg aagtacgaca
     2581 gcagcaagcc ggttggtcag cgggtaatca cactgactct gaatggcaaa cccattgaag
     2641 atgcgacggt ttatcacatt gctactcaga gtttccttgc tgatggtgga gatggtttta
     2701 ccgcctttac cgaagggaaa gcgcgtaaca caacgggcgg ttattacgtt tatcacgcgg
     2761 tggttgatta cttcaaagcg ggcaacacca ttacggatga acagctcaac ggtatgcgcg
     2821 tgaaggatat caaataaaag tgcgcactga attgactcaa ctgtttgtta cccgttgatt
     2881 gcgcagatcc tgtattcggc ctacgcgagg gcgcaggccg aatcagatgc cttaacatca
     2941 ccatccggtg gcaggacgtt gtttttcgaa ttagcccagg tgtcgctgtg ttgtcagaat
     3001 atgagtcaaa acgtgcgagg tggattgcgc tatagagaaa tcttttatcg cctctaatta
     3061 agatgagttt ttttcatctt ccctgatttt tcctcaccat cattggttat ttttcggttg
     3121 acgcccttcg gcttttcctt catctttaca tctggacgtc taaacggata gatgtgcaca
     3181 acacaacata taacgacaag cgattgatga ggtaaggtat gagctttttt cacgccagcc
     3241 agcgggatgc cctgaatcag agtctggcag aagtccaggg gcagattaac gtttcgttcg
     3301 aatttttccc gccgcgtacc agtgaaatgg agcagaccct gtggaactcc atcgatcgcc
     3361 ttagcagcct gaaaccgaag tttgtatcgg tgacctatgg cgcaaactcc ggcgagcgcg
     3421 accgtacgca cagcattatt aaaggcatta aagatcgcac cggcctggaa gcggcaccgc
     3481 atcttacctg tattgatgcg acgcctgccg agctgcggac cattgcatgc gactactgga
     3541 ataacggtat tcgccatatc gtggcgctgc gtggcgacct gccgccggga agtggtaagc
     3601 cagaaatgta tgcttctgac ctggtgacac tgttaaaaga agtggcagat ttcgatatct
     3661 ccgtggcggc gtatccggaa gtacatccag aagcgaaaag tgcccaggcc gatttactca
     3721 acctgagacg aaaagtggat gccggagcca accgcgccat tactcagttc ttcttcgatg
     3781 tcgaaagcta cctgcgtttt cgcgatcgct gcgtctcggc aggcattgat gtggaaatca
     3841 ttccgggaat tttgccggtt tcgaacttta aacaggcgaa gaaatttgcc gacatgacca
     3901 acgtgcgtat tccggcgtgg atggcacaaa tgttcgacgg cctggatgac gatgctgaaa
     3961 cccgcaagct ggtgggcgcg aatattgcca tggatatggt gaagatttta agccgtgaag
     4021 gagtgaaaga tttccacttc tatacgctta accgcgctga aatgagttac gcgatttgcc
     4081 atacactggg agttcgacct ggtttataaa tagtgtgcct tttgtgaaaa tcacacagtg
     4141 ttcacaaatt ttaaacagag cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt
     4201 tatcagcctt gttttctccc tcattacttg aaggatacaa atctaaaacc cttttttata
     4261 aagcatttat cctaaatcgg acataatcaa aaagtcttaa ttacgatcaa tttgatctac
     4321 atctctttaa ccaacaatat gtaagatctc aactatcgca tccgtggatt aattcaatta
     4381 taacttctct ctaacgctgt gtatcgtaac ggtaacactg tagaggggag cacattgatg
     4441 agcacgtcag acgatatcca taacacgaca gccaccggca aatgcccgtt ccatcagggc
     4501 ggtcacgacc agagcgcagg tgggggcaca accacgcgcg actggtggcc aaatcaactc
     4561 cgtgttgacc tgttaaacca acattctaat cgttctaacc cgctgggtga ggactttgac
     4621 taccgcaaag aattcagcaa attagattac tacggcctga aaaaagatct gaaagccctg
     4681 ctgacagaat ctcaaccgtg gtggccagcc gactggggca gctacgccgg tctgtttatt
     4741 cgtatggcct ggcacggcgc gggtacttac cgttcaatcg acggtcgcgg cggcgcgggt
     4801 cgtggtcagc aacgttttgc accgctgaac tcctggccgg ataacgtaag cctcgataaa
     4861 gcgcgtcgcc tgttgtggcc aatcaaacag aaatatggtc agaaaatatc ctgggccgac
     4921 ctgtttatcc tcgcgggtaa cgtggcgctg gaaaactccg gcttccgtac cttcggtttt
     4981 ggtgccggtc gtgaagacgt ctgggaaccg gatctggatg ttaactgggg tgatgaaaaa
     5041 gcctggctga ctcaccgtca tccggaagcg ctggcgaaag caccgctggg tgcaaccgag
     5101 atgggcctga tttacgttaa cccggaaggc cctgatcaca gcggcgaacc actttcggca
     5161 gcagcagcta tccgcgcgac cttcggcaac atgggcatga atgacgaaga aaccgtggcg
     5221 ctgattgcgg gtggtcatac gctgggtaaa acccacggtg ccggtccgac atcaaacgta
     5281 ggtcctgatc cagaagcagc accgattgaa gaacaaggtt taggctgggc gagcacttac
     5341 ggcagcggcg ttggcgcaga tgccattacc tctggtctgg aagtggtctg gacccagacg
     5401 ccgacccagt ggagcaacta tttcttcgag aacctgttca agtatgagtg ggtacagacc
     5461 cgcagcccgg ctggcgcaat ccagtttgaa gcggtagacg caccggaaat tatcccggat
     5521 ccgtttgatc cgtcgaagaa acgtaaaccg acgatgctgg tgaccgacct gacgctgcgt
     5581 tttgatcctg agttcgagaa gatctctcgt cgtttcctca acgatccgca ggcgttcaac
     5641 gaagcctttg ctcgtgcctg gttcaaactg acgcacaggg atatggggcc gaaatctcgt
     5701 tacatcgggc cggaagtacc gaaagaagat ctgatctggc aagatccgct gccgcagccg
     5761 atctacaacc cgaccgagca ggacattatc gatctgaaat tcgcgattgc ggattctggt
     5821 ctgtctgtta gtgagctggt atcggtggcc tgggcgtctg cttctacctt ccgtggtggc
     5881 gacaaacgtg gtggtgccaa cggtgcgcgt ctggcattaa tgccgcagcg tgactgggat
     5941 gtgaacgccg cagccgttcg tgctctgcca gttctggaga aaattcagaa agagtctggc
     6001 aaagcttcgc tggcggatat catcgtgctg gcaggtgtgg ttggcgttga gaaagccgca
     6061 agcgccgcag gtttgagcat tcatgtaccg tttgcgccgg gccgcgttga tgcgcgtcag
     6121 gatcagactg acattgagat gttcgaactg ctggagccaa ttgctgacgg tttccgtaac
     6181 tatcgcgctc gtctggacgt ttccaccacc gagtcactgt tgattgataa agcccagcaa
     6241 ctgacgctga ccgcgccgga aatgactgcg ctggtgggcg ggatgcgcgt actgggtgcc
     6301 aacttcgatg gcagcaaaaa cggcgtcttc actgaccgcg ttggcgtatt gagcaatgac
     6361 ttcttcgtga acttgctgga tatgcgttat gagtggaaag cgaccgacga atcgaaagag
     6421 ctgttcgaag gccgtgaccg cgaaactggc gaagtgaaat acacagccag ccgtgcggat
     6481 ctggtctttg gttctaactc tgtcctgcgt gcggtggcgg aagtctacgc cagcagcgat
     6541 gcccacgaga agtttgttaa agacttcgtg gcagcgtggg tgaaagtgat gaacctcgac
     6601 cgtttcgacc tgctgtaatc tgaccttctt cagcgactgc ctttcatgca gtcgctgaag
     6661 tttctttacc ggcgtatagt gtccacagga aaactacaca ctggatctct catgtctgcc
     6721 gcaggaaaga gcaacccact ggcaatcagt ggcctggttg tgctcacact tatctggagt
     6781 tatagctgga ttttcatgaa gcaagtcacc agctacatcg gtgccttcga cttcaccgcc
     6841 ttacgctgca ttttcggcgc tctcgtttta ttcatcgtcc ttttattacg tggtcgcgga
     6901 atgcgcccga cgccgtttaa atacacctta gccattgccc tgttacaaac ctgcgggatg
     6961 gttggcctgg cacagtgggc gctggtcagc ggcggagcgg ggaaggtggc aatcctgagc
     7021 tataccatgc cgttctgggt ggtaattttc gccgcgttgt ttctcggtga acgcctgcga
     7081 cgtgggcaat atttcgcgat actgattgcc gctttcggct tatttttggt gttgcagccg
     7141 tggcaactcg atttctcttc gatgaaaagt gccatgctgg caatcctctc cggtgtgagt
     7201 tggggggcga gcgcgattgt tgctaaacgt ctgtatgccc gcaatccgcg cgtggattta
     7261 ttgtcgctaa catcctggca gatgctgtat gcggcgctgg tgatgagcgt ggtcgcttta
     7321 ctggtgccgc aacgtgaaat tgactggcaa cccaccgtgt tctgggcgct ggcctacagt
     7381 gcgattctgg cgacagcgct ggcgtggagc ttatggttgt ttgtattgaa aaacttgcct
     7441 gccagtattg ccagcttaag cacactggcc gttcccgttt gcggcgtact cttttcctgg
     7501 tggctgctcg gtgagaatcc gggggcagtt gaaggtagcg gtattgtgct gattgtgctg
     7561 gcactggcgc tggtgagccg taagaaaaaa gaagccgtca gtgtaaaaag gatctgaatt
     7621 ttttcttcat gtggggagat ctcttattta gcaaaatacc gatagtgccc caccatccgc
     7681 cagctaaaca gtacatcttc ttcctgcgcg cctgcgccaa tgttatgtat caccagcggc
     7741 gtaccgtcgc gggcgaagcc atctgacacc accccaatat gtgccagccc gttatctaat
     7801 cgccaggaga caatatcgcc cgcttgatag tcactggggt tcttgctggt ggggcgtgtt
     7861 ttatcgtggc gggtaaacta ggtttccaga ttaggcaccc gacggtgatc gatgttgctg
     7921 tccgggcgct ttaactgcca tttttgcggg tacgcagcaa aattcttcgc catatcttcg
     7981 tgcaccagtt tctgcaaatc gaccttctgg ctgcgcaatg cacggatcac cacatcggaa
     8041 catacgccgc gttcttgcgg gacatcaccg ccaggatagg taagctgcac atacgccgga
     8101 tcgtaaaata gcgtgctgcc gatttgctgt ctggcgccgt ctgcgatggc aagattggtg
     8161 ttagcctgga tttgtaccac ggttggtgga acggcgggag attttaagga gtaactggta
     8221 aataccgtca gaagactgaa taacgccagt gaagctttca tctgtttcgt ccgtgaaaat
     8281 aagagtcaca gattcgacct tccgggctaa cgattgttct gtggagaaaa tgtccagaag
     8341 cggataaaaa aacacccgaa caaggtggaa gtttcagatt actggcacag cgtgccggat
     8401 gcggcgtgaa cgccttatcc ggcctacaaa agtacgtaaa tttactatat tgcagagatt
     8461 gcgtaggcct gataagcgta gcgcatcagg caattttgcg ttcaaactcc cggacaagcc
     8521 gggagtttgg agtaggttat tcccactctt gcaggaaacg ctgaccatac tggtcggcta
     8581 ccagcagtgc ggcgtaaacc tgatctggcg tcgcgccgcc aggcatgttg tggatggttt
     8641 caccttctgc acatgccgct tctgccacaa ttcgcatttt cgccgggaca tcttctttaa
     8701 tatccagttg agcgagagtt attggcaaac ctaccgcatg gctaagcgca gctacggttt
     8761 cgatttcctc caccggcgcg ttttccagaa ccagctgcgt cagcgtaccg aatgccactt
     8821 tttcaccatg ataatagtga tgcgcgtccg ggatagcggt caggccgtta tgtaccgcgt
     8881 gtgccgccgc cagcccgccg ctttcaaaac caacgccact caaataggtg ttcgcttcaa
     8941 tcacgcgctc cagcgccgga gtcactacat gctgttcggc ggcaagcatc gctttttcgc
     9001 cttcttccag cagggtgttg tagcacagtt cagccagtgc cagcgctgcc tgagtgcact
     9061 tgccacccgc catggtggtc gcgccgctac gggaacaggc acgcgcttca aaccaggttg
     9121 ccagcgcatc gccaataccc gccgctaaca gacgcgcggg cgcgccagcg acgattttgg
     9181 tgtcgacaat gaccatattc gggttatttg gcaacagcag atagcggtca aactcaccct
     9241 catcggtgta gataacagac aatgcgctgc acggtgcgtc ggtggaagcg atagtcggtg
     9301 cgatcgctac cggaacaccc atgaaatgtg ccagtgcttt ggcagtatcg agggtttttc
     9361 cgccaccgat accgagaatt gcgccacact gcgcagtctc cgcgatgcca cgcagacggt
     9421 cgatctcatt ttgcgaacat tcaccgccaa acggcgcaat ttctactacc agtccagcat
     9481 ctttaaagct tttctcgaca gtggattgag caaaacctaa aacaaatttg tcacccacca
     9541 ctaaccagcg ttccgccagt ggcttcaggt actcgcccag acgattaatt acatctgcgc
     9601 cctggatgta tttacctggt gattgaataa tgcggtccat aattactcct ttagagatga
     9661 gtagtgccaa atgcggcatt ccagtcgttc tcgaacttct ctatagctga ctctaccgca
     9721 ggggtgttaa gcatttgttg cgctacatct aagggcagtg tgatggattc acatccagcc
     9781 agcaaacaat ccagcgcctg acgtggtgtt ttaaagctgg cagccagcac catgctttct
     9841 ggcgcatgca tttccagtaa cgcttgcaac tcctgtacag tacgaatgcc gtcaccgccc
     9901 tgggcatcta cgcggttaac gtatggagca acgtatttgg ctccagccag cgccgccagt
     9961 aatccttgcg cggcgctgta cactgcggtt cccagcgtag taatgccttc tttcttcagc
    10021 attttaattg ctgccagacc ttcagaggtt accggaattt tcaccacaat gcccggaata
    10081 gcgtcgcgca ggcgtttcgc ttctttcacc ataccctgcg cgtcgcggct catggtctga
    10141 gcaaacagaa tgccctcatc accgatcgct ttttgaaggc gcggcagcag ttcccagatg
    10201 gactccttgc tggcagcgat aatgctcggg ttagttgtca ccccggcaat cgggaatatg
    10261 cgtgccagac gttcgacttc tgcgacgtta gcggtgtcca gatacagttc catgatgttt
    10321 ccttttacag ttccagttca tgttgcagca ggctggcgat agcgtcggca gaggcggcgt
    10381 taaccagcgc attacggaat tcttcgtgca tgatgcgacg agcgaggcgc gagaaaatgc
    10441 gcatatgttg atcgcccgcg gcgtgtttgt tcagggttaa cataatgatg aattgcgctt
    10501 catcatcgcc ccagcgcact ggcgcttgca gacgcgctac gctgatggtg gattgctcaa
    10561 tgtgttccga tttactgtgg ggaatggcga aactaaagcc cagtccggta gagaaaacgg
    10621 cctcgcgcgc ccacaagtcg gcttccagtt tacgcggata gcggcagcgg cccgccagca
    10681 gcaggttatc ggtcatgcct ttgagcactt cttctttgct gcgccagtcg ctttccagtg
    10741 tgatgcactc ggcggtgacc agcggtgcgt cctgttgggt catgcggaat tgcgccagca
    10801 ggtgttccac ttccagcgaa gtacggcagg ccattgcctg gttgagcaac tggcggcact
    10861 cacggctatc aagttgcgcc atccgcgctt tcgccgccgg aattgatggt gcgctcatgc
    10921 tgagttcatc cagccctaaa ccgaccagca acggcagcac ggaacctttc gctcccagtt
    10981 caccgcacag accaatccat ttgccctggc gatgcaccgc ctgcacggcg taatcgagcg
    11041 cccgcaagaa ggccggattc aggctgttgt agtgacgagt caccttagcg ttatcgcgat
    11101 ccactgccag cagatattgc gtcaggtcat tactaccaat actaaagaaa tcaatctctt
    11161 cgcagcattg atcgatgatg aacatcaccg acggcacttc cagcatgatg ccgagctgga
    11221 tcttctcatc aaacggaatg tgttcgttac gcagttgctg tttggcttcc gccagttttt
    11281 cttttaccca taagatctct tccattgagg agatcatcgg gatcatgatt ttcaggctgc
    11341 cgtgagcaga ggcgcgcagg atcgaccgta gttgtgtggt aaacaacgac gcgtattctt
    11401 cataaatacg caccgcgcga taaccgagga acgggttagc ttctgccgga atgttcagat
    11461 aatcaacggg tttatcaccg ccaatgtcca tagtgcgcac aataatgctg cgtccgttgg
    11521 cagactccag cgcctgacaa aaaatgttgt acagctcgct ttcacccggt gcactggtgc
    11581 gatccatata gagcatttca gtgcggaaca aaccaacgcc ttccgcacca ttgccgaatg
    11641 cggcctgcgc ttccacggag tgagcgatgt tagcggcaat ttcaatgcgg ataccgtcag
    11701 cggtacgggc ttgttgggtc agccagacac gctgttgctc acgcagggcg tcctgtacgc
    11761 gggcttcctg ctgataataa cgagctacgg cttcccctgg ctcaaccaca atcgccccag
    11821 cgttgccgtc gatataaatc gtttgatgct gccacggagt gagggcatca atatccacac
    11881 caaccagcgt cggaatgttg aacgaacggg caaggatcac cgtatgtgag gtggtgccgc
    11941 cgcttttcag taatagacct ttgaggtgat ttttatccag ttcgaggaac tggctggggg
    12001 tcagttcatc cgccatacaa atggcgggct gcgtcagttt gcccggtgcc gggaagcgtt
    12061 gctcaccgta gatttgctgg agtaactgga agcagacgtc gcgtacgtcc agggcgcgtt
    12121 cttgcaggta gctgctgctg gaacgggaaa actcttcgca gaagtggtgt gcactggcga
    12181 caatcgcttc ggcacagctt aatccggcgc tgacacctgc cagtaaatgt tcgcgcaggg
    12241 aagtatcgcc agccagggat cggtgcgctt ccagaatcgc gctggtagca ccgtcgctat
    12301 ccagcagacg aaactctatg tttttcagta ccagcgtcag accgttttcc agtgcggatt
    12361 gttcggcgtc aacgcctttg gctgcgggaa gattacccag cgcattgaga tctaaagaag
    12421 agatcggcgt cagaatgccg cctgcgctac cgctgcacac ggtgcgggca cggataattt
    12481 gcggattcag attggtcagt gaaaccggca gtggttccag ttcgtcagat tttacttccg
    12541 ccagcggtgc gtcgcagtgg gggaattcat cgcgcagcca ttggcttaaa cgctggtgcg
    12601 cttcctgttc gtcggccccg gaaatcagta actggcagtt atcgcccacc agcgtatcgg
    12661 tgccaatcag cgcaagggcg cttttggcgt tgcccttgcg gtcagtgcgc aggttatgcc
    12721 actcaatttg tgatgaaaaa gtattacaca gcgtttcaac gtggcttgcc ggacgcgcat
    12781 ggacgccgtt aggtagctca caaataaatt ccacaatcag ggccattgcc tttctcccgg
    12841 tacggttttc tgtttacagg ataagagcgc acggcaacgg cctaccatgt gacaaatctg
    12901 ccaaaagctg gacaaatgta atgtaaccgt caatttgcga cgcgtctcac aagacgccgt
    12961 tttgcggtgt acttccggat tatcgcaagt tatgaggcgg atcgcatttt tgtactgata
    13021 ttacaaaagt ccagtaaatg gcctttttat ccactgtttg ctccgcctgc gattgcctat
    13081 tgtttcttcc atcaaaaata tggacatggg ctttcgcaag cccgggagca gcttatgaat
    13141 gagttggtgc agatcctgaa aaatacccgt cagcatttaa tgacgggcgt ttcacacatg
    13201 attcccttcg tggtatcggg cggtattttg ctggcggttt ccgtcatgtt gtatggcaaa
    13261 ggcgcagtgc cggatgccgt agccgatccg aatctgaaaa aactgtttga tatcggcgtt
    13321 gcaggtttga cgctgatggt gcctttcctc gccgcttaca tcggttattc cattgcagaa
    13381 cgttctgcgc tggctccatg cgctatcggt gcctgggttg gtaacagctt tggcgcgggc
    13441 ttctttggtg cgctgatcgc cgggattatc ggcggcatcg tggtgcatta cctgaagaaa
    13501 attccggtgc ataaagttct gcgctcggtg atgccaatct tcatcattcc aatcgtcggc
    13561 acactgatta ccgctggcgt catgatgtgg gggctgggcg agccggtggg ggcgttgacc
    13621 aacagcctga cccagtggct tcaggggatg cagcagggca gcattgttat gctggcggtg
    13681 atcatgggtc tgatgctggc gttcgatatg ggcggcccgg ttaacaaagt ggcctatgcc
    13741 ttcatgctga tttgcgttgc tcagggtgtt tataccgtgg tggctattgc cgctgttggg
    13801 atttgtgttc caccgctggg gatggggctg gcgacgctga ttggtcgtaa aaatttctcc
    13861 gcagaagagc gcgaaaccgg caaggcggca ctggtgatgg gctgtgttgg cgttactgaa
    13921 ggggcgattc ctttcgccgc tgccgatccg ctgcgtgtca ttccttccat catggtcggt
    13981 tctgtttgtg gtgcggtaac tgcggcgctg gtcggtgcgc agtgctatgc aggctggggg
    14041 ggtctgattg tgctgccggt ggttgaaggt aagttgggtt acatcgcggc agtggctgtc
    14101 ggggcggttg tgacggcagt ctgcgtgaac gtgctgaaaa gtctggcgcg taaaaatggg
    14161 tcttcaactg aagagaagga agacgacctg gatctggatt ttgaaattaa ttaattgagg
    14221 aacgagccat gacgaaaatt atcgcagtaa ccgcatgtcc ttcaggtgtt gcccatactt
    14281 atatggcggc agaggcgctg gaaagcgccg cgaaagcgaa aggctgggaa gtgaaagtag
    14341 aaacccaggg gtcgattggt ctggaaaatg aactgactgc ggaagacgtg gcaagcgccg
    14401 atatggtgat tttgaccaaa gatatcggca tcaaatttga agagcgtttt gctggcaaga
    14461 ctatcgtgcg tgtcaacatc agcgacgcgg taaagcgtgc tgacgccatt atgagcaaaa
    14521 ttgaagctca cctggcacaa accgcttaag ttcttatccc gcccgcaagg gcgggttcgc
    14581 tttcccacag gagttcctca tgacgaatcg tatctctcgc ctcaaaactg cactgtttgc
    14641 caatacccgt gaaatctcgc tggagcgggc gttgctttat accgccagcc atcggcaaac
    14701 cgaaggggag ccggtgataa tgcgtcgggc gaaagcaaca gcgtatatcc ttgaacacgt
    14761 tgaaatttcg attcgtgatg aagaactgat tgccggtaac cgcaccgtaa aaccacgcgc
    14821 cgggattatg tcgccggaaa tggaccctta ctggctgctg aaagagctgg atcaattccc
    14881 gacgcgtccg caggaccgct ttgctatcag cgaagaagat aaacgtatct accgcgaaga
    14941 gttgttcccg tactgggaaa aacgttcgat gaaagatttc atcaacgggc agatgacgga
    15001 tgaagtaaaa gccgcgacca gcacgcagat tttcagcatc aaccagacgg ataaaggcca
    15061 ggggcacatt attattgatt acccgcgcct gctgaatcac gggctggggg agctggtggc
    15121 acagatgcag caacattgtc agcaacagcc ggagaatcac ttttatcagg cagcgctgtt
    15181 actgctggaa gcctcgcaga aacatatttt gcgttacgcc gaactggcgg aaacgatggc
    15241 ggcaagctgc actgatggcc cgcgtcgcga agaactgctg accattgcgg agatctcccg
    15301 ccataacgcc gaacataagc cgcagacgtt ctggcaggcg tgccagttat tctggtacat
    15361 gaacatcatt ctgcaatacg aatccaacgc cagttcgcta tcgttggggc gcttcgacca
    15421 gtatatgttg ccgttctatc agacatcatt aacccagggc gaagatccgg cattcctgaa
    15481 agaactgctc gaatctttat gggtgaaatg caacgacatc gtgctgttgc gctccaccag
    15541 tagcgcgcgt tatttcgccg gttttcctac cggctatacc gcactgctcg gcgggttaac
    15601 cgagaacgga cgtagcgcgg tgaacgtgct ttcgttcctt tgcctcgatg cctatcaaag
    15661 cgtgcaatta ccgcaaccga acctgggtgt gcgcactaac gccttgatcg acacgccgtt
    15721 cctgatgaaa accgcagaaa ctattcgcct cggcaccggt attccgcaaa tctttaacga
    15781 tgaagtagtg gtgccagcgt tcctcaaccg tggcgtttcg ctggaagatg cgcgcgacta
    15841 ttccgtagtg ggctgtgtgg aattatctat tcccggcaga acctacggct tgcatgacat
    15901 cgcgatgttt aacctgctga aagtgatgga aatctgcctg catgaaaatg aaggcaacgc
    15961 tgcgctgact tatgaaggtt tactggaaca gatccgcgcc aagatcagcc actacatcac
    16021 cctgatggtt gagggcagca atatttgtga tatcggccat cgcgactggg ctcctgtacc
    16081 gctactctcg tcttttatca gcgattgtct ggaaaaaggc cgcgatatta ccgatggcgg
    16141 cgcgcgttat aacttctccg gcgtacaggg gatcggtatc gccaacctga gcgattctct
    16201 ccatgcgttg aaagggatga tttttgatca acagcgttta agttttgacg aattgctgtc
    16261 ggtattaaaa gccaacttcg caacgccaga aggcgaaaaa gtccgcgctc gcttaattaa
    16321 ccgcttcgag aaatacggta acgatatcga cgaggtggat aacatcagcg ccgaactgtt
    16381 acgccactac tgcaaagaag tggaaaaata ccagaacccg cgcggcggct acttcacgcc
    16441 aggatcgtat accgtttctg ctcacgtccc gttgggatcg gtggttggcg cgacgccaga
    16501 cggtcgtttt gccggagaac agctggcaga cggcggcttg tcacccatgc tgggccagga
    16561 cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag ctcgataata cgctgctgtc
    16621 taacggtacg ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg aagcgggatt
    16681 acgcaaactg gccgacttct tacgggcgtt tacccagctt aagttgcagc atattcagtt
    16741 taacgtggtg aacgccgaca cgttgcggga agcgcaacag cgcccacaag attatgccgg
    16801 gctggtggtg cgcgttgccg gatacagcgc cttctttgtc gaactgtcga aggagatcca
    16861 ggatgacatc atccgccgga cagcgcatca gctgtaacgt tgtggaaacg cgccgcgatg
    16921 atgtggcgcg cattttcaac attcagcgtt attcactgaa tgacggcgag ggcattcgta
    16981 cggtggtctt ttttaaaggc tgtccgcatc tttgcccatg gtgtgctaat ccggagtcga
    17041 tctccggcaa aatccagacg gtgcgcagag aggcgaaatg tctgcactgt gcgaaatgtt
    17101 tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg gattggtcgc gatatcagcc
    17161 ttgacgctct ggaacgggaa gtgatgaaag atgacatttt ctttcgcacg tccggcggtg
    17221 gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga gtttgcgacc cgttttttac
    17281 agcgactgcg gctatggggt gtgtcatgcg ccatcgaaac tgccggagac gcgccagaca
    17341 gcaagctgtt accgctggcg aaattgtgcg atgaagtgtt gttcgattta aaaattatgg
    17401 atgcggctca ggcgcgggat gtggtgaaga tgaacctgca acgcgtgctg gagaatctgc
    17461 gtttgctggt gagtgagggt gtcaacgtga tcccgcgttt accgctgatc cctggtttca
    17521 cgctcagccg ggagaatatg cagcaggcgc tggacgtact gatcccgctg aatatcaggc
    17581 agatccatct gttaccgttt catcagtacg gcgagccgaa ataccgcctg ctggggaaaa
    17641 catggtcgat gaaagaggtt gctgcgccgt cgtcagccga tgtggcaacg atgcgtgaaa
    17701 tggcagaacg ggccggattt caggttaccg tgggaggtta aaatggcata tctggtggca
    17761 gtaaccgcct gcgtaagcgg cgtggcgcat acttatatgg cggcggaacg gctggaaaag
    17821 ttgtgccagt tagagaagtg gggcgtcagc attgaaactc agggcgcgtt gggaacggag
    17881 aatcgtttag cggacgagga tatccgccgg gcggatgttg cgctgttaat tacggatatc
    17941 gagcttgccg gtgccgagcg ttttgaacat tgtcgctacg tgcaatgcag tatctacgct
    18001 ttcctgcgtg agccgcagag ggtgatgagc gcagtgcgca aagtgctttc tgcgccgcag
    18061 caaacccatc ttattctgga gtagtcggtt tttctgtcag ttggctgtgg tactgccgac
    18121 gatactccga cggcgagcgt tcggtatttt tacgaaacag acggcagaag tagttgctgt
    18181 cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag gtcatagcct ttcaataatg
    18241 tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt aaaaccaatg gcccccgttt
    18301 tttgaaacag gtgcgagagg taatttggcg aaatatagaa cgcctgtgcg acagattcgc
    18361 gggtaagtgc ggaggcatag cgttcgtcga tataatcacg aatagcttcg aatagagcct
    18421 ggctgcgtga ggcggtctgt atttggctgc cgagcaaatc gcggcagtgg ctaagcaggc
    18481 tggtgacgat aagtctggcc gtttgctgct cctgcgactg catttgcatt tcattgagcg
    18541 tttgtagcag aaaagaacca atgcgcgggc cgcgtcgggc gacgtgttgc ttcgccagat
    18601 tttgatattg tttgccatcc cattgcacaa cgctgaagcc gagttgctgt ttgccaaaca
    18661 gcacgctaaa ggtggtggcg ggggattgcc attgtggaaa attccagcca ccagcaggga
    18721 cgtacagcac atcgccggga actaacgctg ctccagcgcc ggtatcaata aactcacctt
    18781 ccagcacaat ttccagccgt ggaaaatcga cctgatacgc aagatcgggc accgggccgt
    18841 tgctgctggc aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg cgggagagca
    18901 gatagctgac gtcgtgatac atgttattcc cttaggtatg tagacgggaa aacagtaact
    18961 ggcattatga aaaaaggcca cctgaaaagt gtggcctgat gggagtgaga tgtaaacatt
    19021 gcggcagttt cagtcgactt gtattgccgg atgcggcgta aacgccttat ccggcctacc
    19081 gccgcgatct gtaggccgga taagacgcgt caagcgtcgc atccggcaaa taacatctgg
    19141 ctgataatta ctgattaccc acctgatcgc catacggcag agtgtcgtaa tcgatcagtg
    19201 cgtttttctt atacgggtta ccaatccagc gggtagtttc tttgaactgc ggattcacca
    19261 ctgaacgggt tggatcgtaa ccgtcgtaag ataagcccgc caaatctgac caggtgtgga
    19321 tcagttccgc caggctgtat ttacgatcaa catcctgtga gaaatcacgc ggatgagtcg
    19381 cttgccattt ttctgacgtc cacagcagga acggaatggt gtacatatga cgcgtcgggt
    19441 tgtcttcatt acgcccctga gttttgtgcg gcggcgtgtc gtaaacctct tcaccgtggt
    19501 cagagaaata aaccaggaaa ccgttcggat cggtcgcttt aaagtcttta atcaggctgg
    19561 caaccacgtg atcgttgtac aggttagcat tgtcataatc attatatgac tccagctctt
    19621 ctgcgtttaa tcccggtgga acatgctctg tattaccgtc aaatttgccc tgatcttccg
    19681 gataacggta cttgtacttg atatgcgtac ccagcagatg aacgatgatc agtttcttcg
    19741 gcgcagggtc cttcagcact tcctggaacg gcttcagcac gttggtgtcg tattcacgcg
    19801 cactctgcgt acgttgctgg ttcatgtagt actgcttatc ggtctggcgc gaaaataccg
    19861 tcagcatggt attgcgggcg gtcatcgtct gctggttggt gatccagaag gttttataac
    19921 ccgcctgttt catcatgttc atcagcgacg gctgcgtcag atacagatcc gggttctttt
    19981 cattggcaaa ggtcagcgcc tgttgcagga tttcaatggt gtacggacga gacgtaacca
    20041 cgttattaaa cacggtcaga ttcggatcgg ttttatgcag cgcatccagc tccggcgtgg
    20101 tttcacgcgg ataaccgtac agactcatgc gtccacgctg ggtcgattcg ccaattacca
    20161 gcactaaggt gcgcggttcg ttacccgatt catctttgaa attagccagc ggcggcaagg
    20221 cattattttc attcagtagc tttgttagcg agtttagttg ctgacgatac tgataatagc
    20281 cggtcaggaa ttgccacggt gcggcaggct ccatacgcga tgccaggtta tccaacgttt
    20341 tctcaaacgg cttgttcttg ataaacgtat tcatggcgat cggatgcaga atcaagccat
    20401 aaagcagggc aaaagagaca acataacgcc acggctttgg aatatagacc gggcgcaggc
    20461 gtgtccacag cagaactgcc accgccgtat aggccagcgc gatgagcaca attttcaggc
    20521 tgaaatactg gcttaaatac tcgctggctt cgttggtgtt ggtttcgaac atcacaaaca
    20581 gaacgctctg cgagaactcc tgaccgtaga tgacgtaata gcacagcgcc gccagagagg
    20641 ccgcccatag caccacgccg atcactgcgg caataatttt aatccgcttc ggaaagagga
    20701 ataccgggat caaccacagc gaactgaata acagcgagtc gcgaatgccg ttagtgccac
    20761 tataaccact gatgtaaata atggcctgta gcagagtaga gaaaaaccaa aagtagagca
    20821 gtgcccaacc cagggctttc cagctaaaaa ggggtttagc ctggacttct gtggaatgca
    20881 tagtaagaac ctgtcttgaa aaaaatatcg ccgaatgtaa cgacaattcc ttaaggatat
    20941 ctgaagggat atccagattt tgaataaaat gcagacagaa atatattgaa aacgagggtg
    21001 ttagaacaga agtatttcag aaaaccctcg cgcaaaagca cgagggtttg caggagagga
    21061 agattagccg gtattacgca tacctgccgc aatcccggca atagtgacca ttaacgcctg
    21121 ctcgacgcga ggatccggtt cctggccttc tttttctgcc tggcgggagc ggtgcagcaa
    21181 ctcggcctgc aatacgttca gcgggtcggt gtaaatattc cgtagctgaa tagactctgc
    21241 aatccacggc agatcggcca tcaggtgaga atcgttggca atcgccagca ccactttgat
    21301 gtcttcttct tgcagattgc gtaactcttt acctaacggc cacagtgctt tgtctaccag
    21361 gcgttggtca tagtattccg ccagccacag gtctgctttg gcgaagacca tctccagcat
    21421 gccgagacgc gtcgagaaga atggccaatc gcggcacatg gcttccagct cgctctgttt
    21481 gccatcttcg accacttttt gcagcgccgt acctgcaccc agccaggcgg ggagcatcag
    21541 gcggttttgc gtccaggcaa aaatccacgg aatggcgcgc agcgactcga cgccgccggt
    21601 cgggcgacgt ttcgccggac gtgaacccaa cggcagttta cccaattctt gttccggcgt
    21661 agcggagcgg aagtaaggca caaaatcttt gttttcacgt acgtagccac ggtagagatc
    21721 gcaggagatg actgacagtt catccataat gcgacgccag ctttctttcg gctcaggcgg
    21781 tggcagcagg ttggcttcca gaatcgcccc ggtgtacagc gacaggctgc tgacggtgat
    21841 ttctggcaga ccatatttaa agcggatcat ctcgccctgt tcggtcacgc gcaggccacc
    21901 tttcaggctt cctggcggtt gtgacagcag cgccgcatgg gcaggtgcgc cgccgcgacc
    21961 aatggaaccg ccgcgaccgt ggaacaacgt cagctcaata cccgcttttt cgcaggtttt
    22021 gattaatgca tcctgtgcct ggtattgcgc ccaggaagct gccatcacgc ccgcatcttt
    22081 tgctgagtcg gaatagccaa tcatcaccat ctgtttgccc tgaatcaggc cgcgatacca
    22141 gtcgatattc agcagctggg tcatgacatc gttggcgttg ttcaggtcat cgagggtttc
    22201 aaacagcgga gcaaccggca tcgcaaaccc gatacccgct tctttcagca gcaggtggac
    22261 agccagcacg tcggacggcg ttttcgccat cgagatcacg taggcggcaa tggagccttg
    22321 cggcgcttcg gcaatcacct ggcaggtatc gagcacttcg cgcgtttcgg cgcttggttg
    22381 ccagttgcgc ggtagaagcg gacgtttgga gttcagttcg cggatcagga acgcctgttt
    22441 gtcggcttct gaccagctct cgtagtcgcc gataccgagg tagcgggtca gctcgcccag
    22501 cgcttcggta tggcgggtgc tctcctgacg gatatcaata cggaccagcg gtacgccgaa
    22561 acatttcacg cggcgcaggg tgtcgagcag atcgccgttg gcgataatac ccatgccaca
    22621 cgcctgaagt gactggtagc aagcgtagag cggttcccac agttcttcgt tttgtgtcag
    22681 caggccttct ggttttggca gttcttcgcc tttcaggcgc gcttccagcc atgcctgtgt
    22741 cgccatcagg cgagaacgca ggtttttcat cagatagcga tacggttctg cggcaccctc
    22801 ttcgccaacc agcgccagca gttcaggggt ggcttcaacc atcgacagtt cagaaaccag
    22861 aacctgaata tctttcagga acaaatcggt ggctttccag cggctgagta gcaggacgtg
    22921 gcgggtgata tcggcagtga cgttcgggtt gccgtcgcgg tcaccgccca tccacgaggt
    22981 aaaacgtacc ggaacaaatt cgacgggcag tttgtagccg aggttctctt ccagttgttc
    23041 gtttagttcg cgcaggtaat tcggtacgcc ttgccacagg ctgttttcta ctacggcaaa
    23101 gccccatttg gcttcatcta ccgggcttgg gcgcagttta cggatttcat cggtatgcca
    23161 tgactgggcg atcaactggc gcaggcggcg catcagctgg ttgtgttcgt agtcggcgat
    23221 atctttgtta tcgagctgtt ttaaacaggc gttcacttcc accattttgt ggatcagtgt
    23281 acgacgggta atttcggttg ggtgagccgt gaggaccagt tccagcgaca gcgattccac
    23341 tgcttttttg atggtgtctt cgctcagttc cggctggttt ttcagtttac gcagggtgcg
    23401 ggcgatcact tccgggttgc tggcagcttc gcctttcggc gaaatgctgt ggtattgctc
    23461 ggcggtgttg gccaggttca gaaactgact aaacgcacgc gcaacgggca gcagctcgtc
    23521 gttcgacaaa ttttgtaagg tggtgagcaa ctcctggcgg ttagcatcat tgccagcgcg
    23581 tgaagatttg gacaacttac ggatagtttc tacgcgttca agaatgtgtt ctcctaacgc
    23641 atccttgatg gtttctccca gcactttgcc gagcatactg acattactac gcaatgcgga
    23701 atattgttcg ttcatattac cccagacacc ccatcttatc gtttgatagc cctgtatcct
    23761 tcacgtcgca ttggcgcgaa tatgctcggg ctttgctttt cgtcgtcttt tataaagcca
    23821 cgtaaaagcg gtgacgtcaa atgctgcgaa atcgcttcag caaacgaata aatagcagga
    23881 atttacgcta ttaaattcac gacgctttaa ataagcgtaa cttatgggaa tgttaaaaaa
    23941 tcgcctcgag taacaccaaa ggtgtaggtc ggataagatg cgtaagcatc gcatccgaca
    24001 ttgtttggcc tacgttttaa tgccagcaaa aatggtgaat cacctgggtg atcagttcgc
    24061 gggtgggctt gataaacctc gtctccagat attcatcagg ctgatgagcc tgattaattg
    24121 agccaggccc cagaaccagc gtcgggcata acgtttgaat aaacggcgct tcggtacagt
    24181 aattcaccac ttcggtcttt gctccgagca atttctcaac cacttcaacc agttgatgat
    24241 tcggtgggca ttcatagcca gggatcggcg gatgcagctc gtcgaccgtc agacgacccg
    24301 gccagcgttc gctcactgga gccaatgcat cgttgagcaa accgttaagt tcattgagtg
    24361 tcatgcctgg cagcggacga atatccatat gcaactcaca acaagcgcaa atacggttag
    24421 atgcatcgcc gccgtggata tgcccgaggt tgagcgtagg gtatggcacg gtaaacgcgt
    24481 cgtagtgata acgttctttc aggttatcgc gcaattgcaa aatatgccct atggcgtcgt
    24541 gcatcagttc gatagcgtta actccgcgtg ctggatcgct ggagtggccc gactggccct
    24601 gaatacggat ggcgttagag atatgacctt tatgcgcgcg taccggttgc agcgacgttg
    24661 gctcgccaat gatggcgcaa tccgggcgca gggcggtagt ttcggcaaaa taacgcgctc
    24721 cggccatact ggtttcttca tcggcagtcg ccagaatgta gagcggtttt gcaagcttcg
    24781 tgacgtcgac atcgcgtagc gcatcaagga taaacgcaaa aaagcctttc atgtcggcgg
    24841 tgcctaagcc gtaaagcttg ccgtcatgct ccgtcaacgt aaatggatcg cgcgtccagc
    24901 gtccgtcatc aaatggcacc gtatcggtat gccccgccag caacaaaccg ccagccccct
    24961 gtccgcaact ggccagcata ttaaatttgt tgcgagttcc tggcacaggc tgtacttcca
    25021 cattgaagcc taaatcctta aaccagtccg ccagcagagt gattaaatct gcattgcttt
    25081 gatcgagtgc ctcttccgtg gcgcttattg aaggtgtggc aatcagagca cggtaaatct
    25141 cgataaatgg cggtaatttg tttttcattg ttgacacacc tctggtcatg atagtatcaa
    25201 tattcatgca gtatttatga ataaaaatac actaacgttg agcgtaataa aacccaccag
    25261 ccgtaaggtg aatgttttac gtttaacctg gcaaccagac ataagaaggt gaatagcccc
    25321 gatgttgaat acgctgattg tgggtgccag cggctacgct ggcgcagagc tagtgaccta
    25381 tgtaaatcgc catccgcata tgaacataac cgctttgact gtttcagcgc aaagcaatga
    25441 tgcgggaaag ttaatctccg atttgcatcc gcagctaaaa ggcatcgttg atctgccgtt
    25501 gcagccgatg tcggatatca gcgagtttag cccaggggtg gacgtggtgt ttctcgccac
    25561 cgcccatgaa gtcagccacg atttagcgcc gcaatttctt gaagcaggct gtgtggtgtt
    25621 cgacctttcc ggcgcgtttc gtgttaacga tgtcgccttc tatgaaaaat attacggctt
    25681 tacccatcaa tacccggaac tgctggaaca ggcagcctac ggtctggcgg agtggtgcgg
    25741 taataaatta aaagaagcga acttgattgc cgtgccaggc tgttatccga cggcagcaca
    25801 gctggcgctg aaaccgttga ttgatgccga tcttcttgac ctcaatcagt ggccggtgat
    25861 caacgccacc agcggcgtga gcggtgcagg acgtaaggca gcgatttcaa atagtttttg
    25921 tgaagttagc ctgcaaccgt atggcgtctt tacccatcgc catcaaccag agatcgccac
    25981 acacctcggt gctgacgtta tcttcacccc gcatctgggc aatttcccgc gcggcattct
    26041 cgaaaccatt acctgccgcc tgaaaccggg tgtgagccag gtgcaagtgg cgcaagcgtt
    26101 acagcaggcg tatgcccata aaccactggt gcggctgtat gacaaaggcg ttccggcgct
    26161 gaaaaatgtc gttggactgc cattctgcga tatcggtttt gccgttcagg gcgagcatct
    26221 gattattgtg gcgaccgaag acaacttact gaaaggcgcg gcggcacaag cggtgcagtg
    26281 cgccaatatt cgtttcggct atgcggaaac gcagtctctt atttaagggt gcaatgatga
    26341 atccattaat tatcaaactg ggtggcgtac tgctggatag tgaagaggcg ctggaccgtc
    26401 tgtttagcgc actggtgaat tatcgcgagt cacatcagcg tcagctggtg attgtgcacg
    26461 gcggcggttg cgtggtggat gagctgatga aagggctgaa tctgccggtg aaaaagaaaa
    26521 atggcctgcg ggtgacgcct gcggatcaga tagacattat caccggagca ctggcgggaa
    26581 cggcaaataa aaccctgctg gcatgggcga agaagcatca gattgcggcc gtaggtttgt
    26641 ttctcggtga cggcgacagc gtcaaagtga cccagcttga tgaagagtta ggccatgttg
    26701 gactggcgca gccaggttcg cctaagctta tcaacagctt gctggagaac ggttatctgc
    26761 cggtggtcag ctccattggc gtaacaaacg aagggcaact gatgaacgtc aatgccgacc
    26821 aggcggcgac ggcgctggcg gcaacgctgg gcgcggatct gattttgctc tctgacgtca
    26881 gcggcattct cgacggcaaa gggcaacgta ttgccgaaat gaccgccgcg aaagcagaac
    26941 aactgattga gcagggcatt attactgacg gcatgattgt gaaagtgaac gcggcgctgg
    27001 atgctgcgcg cacgttgggc cgtccggtag atatcgcctc ctggcgtcat gcggaacagc
    27061 ttccggcact gtttaacggt atgccgatgg gtacgcggat tttagcttaa gttttgttgg
    27121 ccggaggcgc agctttccgg cattgaattt caaaataagg aaacagagtt atggcacttt
    27181 ggggcgggcg ttttacccag gcagcagatc aacggttcaa acaattcaac gactcactgc
    27241 gctttgatta ccgtctggcg gagcaggata ttgttggctc tgtggcctgg tccaaagccc
    27301 tggtaacggt cggcgtgtta accgcagaag agcaggcgca actggaagag gcgctgaacg
    27361 tgctgctgga agatgttcgc gccaggccac aacaaatcct tgaaagcgac gccgaagata
    27421 tccatagctg ggtggaaggc aaactgatcg acaaagtggg tcagttaggc aaaaaactgc
    27481 ataccgggcg tagccgtaat gatcaggtag caactgacct gaaactgtgg tgcaaagata
    27541 ccgttagcga gttgctgacg gctaaccggc agttacaatc cgctctggtc gagaccgcgc
    27601 agaacaatca ggacgcggtg atgccgggtt acactcacct gcaacgcgcc cagccggtga
    27661 cgttcgctca ctggtgcctg gcctacgttg agatgctggc gcgtgatgaa agccgtttgc
    27721 aggacgcgct taagcgtctg gatgtcagcc cgctaggttg tggcgcactg gcaggaacgg
    27781 cctatgaaat cgaccgtgaa cagttagcag gctggctggg ctttgcttcg gcgacccgta
    27841 acagtctcga cagcgtttct gaccgtgacc atgtgttgga actgctttct gctgccgcta
    27901 tcggcatggt gcatctgtcg cgttttgctg aagatctgat tttctttaac accggcgaag
    27961 cggggtttgt ggagctttct gaccgtgtga cttccggttc atcattaatg ccgcagaaga
    28021 aaaacccgga tgcgctggag ctgattcgcg gtaaatgcgg tcgggtgcag ggggcgttaa
    28081 ccggcatgat gatgacgctg aaaggtttgc cgctggctta caacaaagat atgcaggaag
    28141 acaaagaagg tctgttcgac gcgctcgata cctggctgga ctgcctgcat atggcggcgc
    28201 tggtgctgga cggcattcag gtgaaacgtc cgcgttgcca ggaagcggcg cagcagggtt
    28261 acgccaacgc tactgaactg gcggattatc tggtggcgaa aggcgtaccg ttccgcgagg
    28321 cgcaccatat tgtgggtgaa gcggtggtgg aagccattcg tcagggcaaa gcgttggaag
    28381 atctgccgct cagtgagttg cagaaattca gccaggtgat tggcgaagat gtctatccga
    28441 ttctgtcgct gcaatcgtgc ctcgacaagc gtgcagcaaa aggcggtgtc tcaccgcaac
    28501 aggtggcgca ggcgattgct tttgcgcagg cgcggttggg gtaatttgtt tatattgctc
    28561 ccctgaaaat ggggagctta ccacgcctac aaagtgtgac acttcgtagg cggataaggc
    28621 gtttacgccg catcaggcag aaatttgtca gtattctggt gaccttaaat ttaacgccca
    28681 accagaacca ttatttgtca cattcctttt tattcttgcg tcttctttac cttcact
//
LOCUS       DADTFG010000055        28577 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.055, whole genome shotgun sequence.
ACCESSION   DADTFG010000055 DADTFG010000000
VERSION     DADTFG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.055"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(138..1358)
                     /locus_tag="J1138_002146"
     CDS             complement(138..1358)
                     /locus_tag="J1138_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8453946.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1576..3228
                     /locus_tag="J1138_002147"
     CDS             1576..3228
                     /locus_tag="J1138_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBA8453947.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQNKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3265..3744)
                     /gene="ybaK"
                     /locus_tag="J1138_002148"
     CDS             complement(3265..3744)
                     /gene="ybaK"
                     /locus_tag="J1138_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBA8453948.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(3948..4742)
                     /locus_tag="J1138_002149"
     CDS             complement(3948..4742)
                     /locus_tag="J1138_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453949.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACETLEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4875..5176
                     /locus_tag="J1138_002150"
                     /pseudo
     CDS             4875..5176
                     /locus_tag="J1138_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5212..5553
                     /locus_tag="J1138_002151"
     CDS             5212..5553
                     /locus_tag="J1138_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBA8453950.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5611..8115)
                     /gene="copA"
                     /locus_tag="J1138_002152"
     CDS             complement(5611..8115)
                     /gene="copA"
                     /locus_tag="J1138_002152"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBA8453951.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALVVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8378..9310
                     /gene="glsA"
                     /locus_tag="J1138_002153"
     CDS             8378..9310
                     /gene="glsA"
                     /locus_tag="J1138_002153"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBA8453952.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            9313..10605
                     /locus_tag="J1138_002154"
     CDS             9313..10605
                     /locus_tag="J1138_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA8453953.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELDKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            10730..11137
                     /locus_tag="J1138_002155"
     CDS             10730..11137
                     /locus_tag="J1138_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HBA8453954.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(11138..12361)
                     /locus_tag="J1138_002156"
     CDS             complement(11138..12361)
                     /locus_tag="J1138_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453955.1"
                     /translation="MFSSFRKPEIRLLKNTAAGVIFSVKSGKGFLRQCVIYDPHSHGL
                     IHSLCWQDVPLIRFWSEAGCPTCAEFVYSGFAEDEQGAARFLSALENWNRPWSGITDA
                     FAALTPLFSCMADGYYLLEDRELYPTDGNGHFFWAATDHSSSNPATVAVWDPEYCSFS
                     DTAPCFLLPGQPPSHFNPERATFYRDKPDARALAWYLPDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATHFNDEQQTLVFPGGECLHKTELQYHIPKLTACKTLPPSAWESFGP
                     DKHISPSETWSEELKQSVSRYPSLDQAWQIVEAGNLSETRIKSMIQQGLGEDEKADII
                     LQALFSTHSPLFIDFARFVISHPAYAIYRPLTFRLMAQNRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKP"
     gene            complement(12479..12937)
                     /locus_tag="J1138_002157"
     CDS             complement(12479..12937)
                     /locus_tag="J1138_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBA8453956.1"
                     /translation="MMELMVVHQHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGQRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(12934..13851)
                     /locus_tag="J1138_002158"
     CDS             complement(12934..13851)
                     /locus_tag="J1138_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBA8453957.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            13997..14674
                     /gene="fetA"
                     /locus_tag="J1138_002159"
     CDS             13997..14674
                     /gene="fetA"
                     /locus_tag="J1138_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBA8453958.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            14661..15440
                     /gene="fetB"
                     /locus_tag="J1138_002160"
     CDS             14661..15440
                     /gene="fetB"
                     /locus_tag="J1138_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBA8453959.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(15503..16357)
                     /gene="cnoX"
                     /locus_tag="J1138_002161"
     CDS             complement(15503..16357)
                     /gene="cnoX"
                     /locus_tag="J1138_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBA8453960.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(16418..17227)
                     /gene="ybbO"
                     /locus_tag="J1138_002162"
     CDS             complement(16418..17227)
                     /gene="ybbO"
                     /locus_tag="J1138_002162"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBA8453961.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(17217..17840)
                     /gene="tesA"
                     /locus_tag="J1138_002163"
     CDS             complement(17217..17840)
                     /gene="tesA"
                     /locus_tag="J1138_002163"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBA8453962.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            17811..18497
                     /locus_tag="J1138_002164"
     CDS             17811..18497
                     /locus_tag="J1138_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8453963.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVRLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEV"
     gene            18494..20908
                     /locus_tag="J1138_002165"
     CDS             18494..20908
                     /locus_tag="J1138_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8453964.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLVLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFAG"
     gene            complement(21149..22243)
                     /gene="mnmH"
                     /locus_tag="J1138_002166"
     CDS             complement(21149..22243)
                     /gene="mnmH"
                     /locus_tag="J1138_002166"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBA8453965.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(22312..23238)
                     /gene="allS"
                     /locus_tag="J1138_002167"
     CDS             complement(22312..23238)
                     /gene="allS"
                     /locus_tag="J1138_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBA8453966.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAAEHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            23468..23950
                     /gene="allA"
                     /locus_tag="J1138_002168"
     CDS             23468..23950
                     /gene="allA"
                     /locus_tag="J1138_002168"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBA8453967.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            24028..24843
                     /gene="allR"
                     /locus_tag="J1138_002169"
     CDS             24028..24843
                     /gene="allR"
                     /locus_tag="J1138_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBA8453968.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            24933..26714
                     /gene="gcl"
                     /locus_tag="J1138_002170"
     CDS             24933..26714
                     /gene="gcl"
                     /locus_tag="J1138_002170"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBA8453969.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAAIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            26727..27503
                     /gene="hyi"
                     /locus_tag="J1138_002171"
     CDS             26727..27503
                     /gene="hyi"
                     /locus_tag="J1138_002171"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBA8453970.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            27603..28481
                     /gene="glxR"
                     /locus_tag="J1138_002172"
     CDS             27603..28481
                     /gene="glxR"
                     /locus_tag="J1138_002172"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBA8453971.1"
                     /translation="MTLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLDAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
BASE COUNT         6802 a         7248 c         7687 g         6840 t
ORIGIN      
        1 attgttgaat tttgcagaaa aatggaatta gctgcaaaaa aagcacggat cggaaaacag
       61 aaggcggttt aacagaggaa aggcttatgg cgactgtaaa acgatgcagc caaagtttgc
      121 ctttggctgc aatgaaatca gtctttatgc cggttatcag gcaggaatat ggtcaacatc
      181 cccaatagtg gcaggaaagc acagatttta tagactaact ctatgctggt gtggtcggcg
      241 ataagcccca gaactgccgc ccccagacct cccatgccga aagcaaaacc gaaaaacagt
      301 ccagaaacca taccgatacg tcccggaagc agctcctggg cgtagaccag aatggcagag
      361 aatgctgaag cgaggataaa tccaataatc accgttaaaa cccccgtcca gtgcaggctg
      421 gcgtagggta aaatcagcgt aaacggcgca acgccgagga tagagcccca aatcacatat
      481 ttccgtccaa ttttatcccc tacaggcccg ccaatcaccg tacctgccgc aacggcaaac
      541 aggaaggcaa acaaatgaag ctgagcattc tggatagata atccgaattt ttgcatcaga
      601 taaaaggtgt aatagctgct gatgctcgcc atatagaaat atttcgagaa aatgaggatt
      661 aacagaatgc tgaccgccag aacaacttta ttgcgcggca gtggattgat aatcgtcgct
      721 ttgggttttc ctttattcat tcggtgctgt gccgagtacc aacggctgat ttgcgccaac
      781 accacgatcg ccagcagtgc cgcaagcaca aaccaggcaa cgttgccttt gccataaggc
      841 gcgataatca ccgccgccag caaggggccc agggaactgc caaagttgcc gccgacctga
      901 aagatagatt gcgccaggcc atgacgcccg ccggaagcca tacgggccac gcgagaagat
      961 tccggatgaa agaccgatga accggtaccg accagcgccg ctgccagcag aactgcgcca
     1021 aaactgcccg ccagcgccag cagcaccaga ccacttaagg taaaacacat gccaatcggc
     1081 aacgaccacg gcatcggata tttatcggtc cagtagccga ccaccggttg cagtagcgaa
     1141 gaggcgagct ggaaggtgag ggttatcatg ccaatctgca taaatgtcag agaaaattct
     1201 gactgaagca gtggataaat cgccagaatc agcgattgga tcatgtcgtt cagcagatgt
     1261 gagaggctga tagcacctaa aataccaaac gatgttcggg ccttagtcgt tgacgcagcc
     1321 gcgcccgcca caggctgggt ttgttcactc attgccatag gaaagtcact ttttcagggt
     1381 tgcgatgtaa agaatgatct tatttgtgat tattacccga ctaacatacc tgtatgcgcc
     1441 gcctgaagga agtctcaacg cggaatacag aatttctaat ctggatgcag atttatcttc
     1501 accggacgca gacttgttta tgatgtcgcg tcatactatt tttcaacacg ttgaaatcag
     1561 gtcagggaga gaagtatgaa attattgcag cggggcgtgg cgttagcact gttaaccaca
     1621 tttacactgg cgagtgaaac tgctctggcg tacgagcaga ataaaaccta caaaattaca
     1681 gttctgcaca ccaatgatca tcatgggcat ttttggcgca atgaatatgg tgaatatggt
     1741 ctggcggcgc aaaaaacgct ggtggatggt atccgcaaag aggttgcggc tgaaggcggt
     1801 agcgtgctgc tactttccgg tggcgacatt aacactggcg tgcccgagtc cgacttacag
     1861 gatgccgaac ctgattttcg cggtatgaat ctggtgggct atgacgcgat ggcgatcggt
     1921 aatcatgaat ttgataaccc gctcaccgta ttacgccagc aggaaaagtg ggctaagttc
     1981 ccgttgcttt ccgccaatat ctaccagaaa agtaccggcg agcgcctgtt taaaccatgg
     2041 gcgctgttta agcgtcagga tctgaaaatt gccgttattg gcctgacgac ggatgacaca
     2101 gccaaaattg gtaatccgga atatttcact gatatcgaat ttcgtaagcc cgctgatgaa
     2161 gcgaagctgg tgattcagga gctgcaacag acagaaaagc cagacattat tatcgcggcg
     2221 acccatatgg ggcattacga caatggtgag cacggctcta acgcaccggg cgatgtggag
     2281 atggcacgcg cgctgcctgc cggatcgctg gcgatgatcg tcggtggtca ctcgcaagat
     2341 ccggtctgta tggcggcaga aaacaaaaaa caggtcgatt acgtgccggg tacgccatgc
     2401 aaaccggatc aacaaaacgg catctggatt gtgcaggcgc atgagtgggg caaatacgtg
     2461 ggacgggctg attttgagtt tcgtaatggc gaaatgaaaa tggttaacta ccagctgatt
     2521 ccggtgaacc tgaagaagaa agtgacctgg gaagacggga aaagcgagcg cgtgctgtac
     2581 acccctgaaa tcgctgaaaa ccagcaaatg atctcgctgt tatcaccgtt ccagaacaaa
     2641 ggtaaagcgc agctggaagt gaaaataggc gaaaccaatg gtcgtctgga aggcgatcgt
     2701 gacaaagtgc gttttgtaca gaccaatatg gggcggttga ttctggcagc acaaatggat
     2761 cgcactggtg ccgactttgc ggtgatgagc ggaggcggaa ttcgtgattc tatcgaagca
     2821 ggtgatatca gctataaaaa cgtactgaaa gtgcagccat tcggcaatgt ggtggtgtat
     2881 gccgacatga ccggtaaaga ggtgattgat tacttgaccg ccgtcgcgca gatgaagcca
     2941 gattcaggtg cctacccgca atttgccaac gttagctttg tggcgaaaga cggtaaactg
     3001 aacgacctga aaatcaaagg cgaaccggtc gatccggcga aaacttaccg catggcgaca
     3061 ttaaacttca atgccaccgg cggggatggc tatccgcgcc ttgataacaa accgggctat
     3121 gtgaataccg gctttattga tgccgaagtg ctgaaagcgt atatccagaa aagctcgccg
     3181 ctggatgtga gtgtttatga accgaaaggt gaggtgagct ggcagtaatc cgaaagtgcc
     3241 ggatgttcgc atccggcaca atgcttaatc gcgacgggcg atatcagcaa atttggcatc
     3301 gagtatcttt gccaggtcgc ttgccgccag ttcgatatcc agaccgcgct tgccgccgga
     3361 aacataaata gtggcaaatt cttgtgcggg ggcgtcgata atcgttggca gacgtttttt
     3421 ctgccccagt gggctaatcc ctccaaccag atatcccgtc gaacgctgcg cgaccatcgg
     3481 atcggccatc tcaactttct tggcacccag cgcttttgct acttttttaa gatccagttg
     3541 acctgcgacc ggcgtaacgg ccacggcaag gtgtttcata tcaccgttca ctgcgaccag
     3601 cagcgttttg tagacctgat ccggattcaa acctaatttt ttgacgactt catcgccaaa
     3661 attggtttca gccggatcgt gctcgtaggt atggatttga aacgaaatct tgtttttttc
     3721 gagtaattta actgcgggtg tcatggtaat tttcctgcct taaacaaaaa aacgaagcaa
     3781 gcatacgcct gatggcggtc caaaaaaaag agtcatcttg cctaagagta ttggcaggat
     3841 ggtgagattg agcgacaatc gagttacacc gtcgcttaaa gtgacggcat aataataaaa
     3901 aaatgaaatt cctctttgac gggccaatag cgatattggc cattttttta gcgcaacatc
     3961 tgcggcaaat tcccttctcc atacaggtgt agtgcaccga ctgcgaccac atagcgcccc
     4021 ggcggcatgg cgcgtaattt atcccgccag gcgagatttc gctgatgcat cagcacatcg
     4081 tacagcgact gactgaacgt attgggcagc gttatttcat tattttgcgg cggcgcattc
     4141 aaccaccagc tcatcatttg ttgcagcaac cgtgcattgg tgtgccagtg ggtcagcgtg
     4201 tcgtccagca gcgccagtcc gttgtcaggg agctggagca acatggcaat ctggttttca
     4261 gccccttcca gttcaatcac gggtttatgt tgttgcttcg ccgcctgcaa tagctggtaa
     4321 tcgataccgt attctgcccg cagccccagt ttttgtgcct gcgtcgcctg aagaaccatc
     4381 gcgatttgcc acagcggttg agtagaaaag agcgacggag aaatgcccat ctcctggcta
     4441 atatgctgca gattttggag ttgctcctcg ctaatgcgct cttccagcgt ctcgcaggca
     4501 ggcaaattag caaaaggcgt atcgctggtg gaaacatccg cctcgacgat cagcgcatcg
     4561 gcgtttttga gctttttgag caaacgggtg ggcaggggag ccatatcgtg gctacccata
     4621 tgaatactgc cgatcagatg aaaatggcga ttgccgggaa gagagatatc gatagcaggc
     4681 caggtgtaat gattaccgcg cagcgcggcc caaagcgttt ttacccggta caacagatcc
     4741 atacgacctc cctttgtgaa atatcatgct agcgcgcggg gagggatggc gcaaatggag
     4801 ttgattgttg cacttgtgca cagtgcttgt gtgcattaca atgattaaat aatggacaag
     4861 gagatgagag aggaatggct caaaagaaaa atataaggag ttttcgtgat gcctggttgg
     4921 ctgatttttt gtccattcaa cgccacatcg aaagatccct gctgaaatcc ataccacgtt
     4981 atcacgaaag ttggacatca ttaatgctgc cacttctcat cgggatttac gctcccctcc
     5041 ggggaaccga tacgaagagt tatccgggaa attacaggaa tactcctcca ttagagtgaa
     5101 taaacagtac cgactgattt ttaaatgggt taatggcaag gccgaagaat tgtttttaga
     5161 cccacacaac tactaaaaca tagctgtcgt attgacgaga aaggtaaaac tatgaaacag
     5221 gcaacaagaa aaccgacgac acccggagat attcttctct atgaatatct ggaaccgctc
     5281 gatttgaaaa tcaatgagtt agcagagttg ctgcatgttc atcgtaatag cgtcagtgca
     5341 ctgatcaata acaatcgtaa actcaccact gagatggcat tccgtctggc gaaagttttt
     5401 gataccacag tcgatttttg gctaaacctc caggcggcag ttgatctttg ggaagttgaa
     5461 aacaacatgc gtacccagga agaattggga cggattgaaa cagtggctga atatttggca
     5521 cgccgtgaag agcgtgcaaa aaaggtcgcg tagaataatt tccctataac ggatgcgcca
     5581 ccctaaagca gcgcatccgc aatgatgtac ttattccttc ggtttaaacc gcagcaatcg
     5641 gttggcgtta ctcactacgg tgatcgacga gagcgccatt gccgctccgg caaccaccgg
     5701 gttaagcagt gttccagtga acggccacaa aataccggcg gcgaccggaa taccaatgct
     5761 gttgtagata aacgcaccga gcaggttctg cttcatgttg cgcagcgttg cgcgggaaat
     5821 tgcgagcgca tccgcaacac ccatcaggct atggcgcatc agggtaatcg ccgcggtttc
     5881 aatggcaaca tcactgccgc cacccatcgc aataccgaca tccgcctgag ccagcgctgg
     5941 cgcatcgtta atgccgtcgc ccaccattgc cacctgacgt ccttcacttt gcagacgttt
     6001 gatcgcttcg gctttaccgt ctggcagcac gccggctatc acctcatcaa tcccggcttc
     6061 tttagcgatc gcattggcgg tggtcgggtt atccccggtt agcatcacca ggcgatatcc
     6121 cgctttatgc aggcgttgca gcgccgccac gctatcacta cgcaacggat cgcgtacagc
     6181 cagcagggct actgctttcc cgtcaaccgc cagcagcaca ggcgttgccc cttgcgatgc
     6241 ctgagctgaa atatctgctt caattgcttt ggtatcgacc tgttggtcat ttaacagcgc
     6301 ctgattgccc agcaataacg catgaccttc agcttcaccg ctcacgccca gcccgcgcaa
     6361 tgtgcggaaa ccgttgacct gcggtagctg catatcacct gctttatcga ggatcgcccg
     6421 tgccagaggg tggctggaac cttgctccag cgccgccgcc agacgcaatg cttgcgcttc
     6481 atcaatatca gcaaatgttt ttactgcgac aacctgcggc ttcccttcgg tcagcgtccc
     6541 ggttttatcg aacactacgg tgtcgagcgt actggcgcgt tgcagcgcgt cggcgtcccg
     6601 taccagcacg ccaaactcag ccgcccgccc gacgccggaa ataatcgaca ttggcgtcgc
     6661 cagccccagc gcacacggac aggcaataat cagcaccgtg gtggcaatca ccagagtata
     6721 gacaatctgc ggtgccggac caaagaaata ccagattgcc gcactgatca gcgcaatcac
     6781 caccactacc ggcacaaata cggctgagat tttatccgcc agctgaccga tttctggctt
     6841 actgctctgg gcctggcgca ccatacgaat aatccgcgac agcgtagtat ggctgccaac
     6901 cgcactggcg cgaaacagca cgctaccgtc ctgcaccact gtcccggcat ggacgctatc
     6961 gccttcgcct ttttgctgcg gtataggttc gcccgtcagc atcgcttcat ccagccatgc
     7021 ttcgccctgg gtaatctcgc catctactgg cacgcgatcg ccggtcgtca ggcgcagcaa
     7081 catacctggt tgcacttccg ccagcggcac gcttttttca ccttcgtcag taaccaggcg
     7141 cgccgtcggc ggagttaaat cgagtaattt ctccagcgcc tttgaagaac gctgacgtgc
     7201 gcgcgcttcc agcatatgac cgagattaat cagaccgata atcatcgcac tggcttcata
     7261 gtaaagatgc cgtgcttcca tcgggaacca ctgcggccac aggttgacgc tcatcgaata
     7321 gagccacgca acacccgttc ccagcgccac cagcgtatcc atcgtcgccg caccgttcag
     7381 caggcttttc catgcgctgc ggtaaaaatg cccgccggcg aaaaccatta ccgccagggt
     7441 tatcaggccg ataactaacc acaggctgcg gttgtcagcg gtgaccatca tgttatcgcc
     7501 gatcatcccc cagaccatca ccgggatacc caccaccagc gcgacaattg cctgccagcg
     7561 gaagcgcttc atcgtagcga cggcggtttc ttgctggcgc tcgcggcgtt tagcgtcatc
     7621 ttcaatcgct tccgcgccgt agcccgcttt ttccaccgcc tgcactaaat cttgtgggga
     7681 ggcactgccc atcaccagcg cagtacgctc cgccaggttt acccgtgcct gagtgacgcc
     7741 cggtacgctt tgcaacgcat tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct
     7801 cagcagcaac tgctggctgt catcgtcatc ggcggtcgct gccggaagcg cctcagaaac
     7861 cgctgtcagt gcttccgacg ggattgatga ctccgccagc ggtttagcct ttgggtggct
     7921 tacagatgcg tcataacccg cttgtttgat ggtttcgatt agctgttctg cactggcagt
     7981 cccggtaacg tgcgcttcag tgatagacac atccgcctgt tcaacatccg ggcgctgttc
     8041 gagactttct ttcacgcgtt taacgcagtg accgcaggac aggccgtcca gggtcaggtc
     8101 gatagtttgt gacataaaac actcctttta agacagtttt gactggctgt gataaaggtt
     8161 aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc aggcgggtaa aagtccgtaa
     8221 agattaaaaa atcggctcga tttgcatcag gattagacat ttatctcttt gttttcctgt
     8281 agttaagttg cgggtgctaa gttaaatcag gatgcatgaa aatcggcacc ggggtgagga
     8341 attacctccc gcatctataa aaaggagtta acaaaagatg ttagatgcaa acaaattaca
     8401 gcaggcagtg gatcaggctt acacccaatt tcactcactt aacggcggac aaaatgccga
     8461 ttacattccc tttctggcga atgtccctgg tcaactggcg gcagtggcta tcgtgaccag
     8521 cgatggcaac gtctatagtg cgggtgacag tgattaccgc tttgcgctgg aatccatctc
     8581 gaaagtctgt acgttagccc ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa
     8641 agttggcgct gacccgaccg gattgccctt taactcagtt atcgccttag agttgcatgg
     8701 cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt gccaccacca gcctgattaa
     8761 cgctgaaaat actgaacaac gctggcagcg aattttacat atccaacaac aactggctgg
     8821 tgagcaggta gcgctctctg atgaagtcaa ccagtcggaa caaacaacca acttccataa
     8881 ccgggccatt gcttggctgc tgtactccgc cggatatctc tattgtgatg caatggaagc
     8941 ctgtgacgtg tacacccgtc agtgctccac gctcatcaat actattgaac tggcaacgct
     9001 tggcgcgacg ctggcggcgg gtggtgtgaa tccgctgacg cataaacgcg ttcttcaggc
     9061 cgacaatgtg ccgtacattc tggccgaaat gatgatggaa gggctgtatg gtcgctccgg
     9121 tgactgggcg tatcgcgttg gtttaccggg caaaagcggt gtaggtggcg gtattctggc
     9181 ggtcgttcct ggcgtgatgg gaattgccgc gttttcacca ccgctggacg aagaaggcaa
     9241 cagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag caactcggct ataacgtgtt
     9301 taagggctga tcattatgaa cacggaaggt aataacggta acaaacccct cggtctatgg
     9361 aacgtcgttt ccatcggcat tggggcaatg gtgggggcgg ggatcttcgc gctgctgggg
     9421 caggctgcgt tgctaatgga agcctcgacc tgggtcgcct ttgcttttgg cggtattgtg
     9481 gcgatgtttt ccggttatgc ctatgcgcgt ctgggggcga gctatcccag caatggcggt
     9541 attatcgact tctttcgtcg cggattaggc aacggcgttt tttcgctggc gctctcgtta
     9601 ttgtacctgt tgacgctggc ggtgagtatc gccatggtcg ctcgtgcttt tggcgcctat
     9661 gccgtgcagt ttttgcatga aggcagccag gaggagcacc ttattttgct ctacgcgttg
     9721 gggatcattg cggtgatgac gcttttcaac tccttaagca accatgcggt agggcggctg
     9781 gaagtgatcc tcgtcggcat taaaatgatg atcctgttat tgctgattat tgccggtgtc
     9841 tggtcgctgc aaccggcgca tatttccgtt tctgcgcccc ccagctccgg ggcgtttttc
     9901 tcctgtatcg ggataacctt ccttgcctat gcgggctttg gcatgatggc gaacgcggcg
     9961 gataaagtga aagatccgga ggtcataatg ccacgggcgt ttctggtggc gattggcgtt
    10021 accacgttgc tttatatctc gctggcgctg gttttgctta gcgatgtatc ggcattagag
    10081 ttagacaaat atgccgatac cgccgtagcg caggctgctt ctccgctcct cgggcatgtg
    10141 gggtatgtga tcgtcgtcat cggcgcttta ctggcgacgg cttcagccat caacgcaaac
    10201 ctgttcgccg tgtttaacat catggacaac atgggcagcg aacgcgaact gccgaagcta
    10261 atgaataaac ctctgtggcg gcagagtact tggggcaaca tcatcgtcgt ggtgttgatt
    10321 atgctgatga cggcggcgct gaatttaggc tcactcgcca gcgttgccag cgccaccttt
    10381 ttgatctgct atctggcggt gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc
    10441 tcgttgccga ttcttatcgt tggtacgttg gttatgttgt tggtgatcgt tggctttatc
    10501 tacagtctct ggtcccaggg cagccgtgcg ctgatatgga ttattggcgc actcttactc
    10561 agccttattg tggcaatggt catgaagcgc aataaaaccg tataacatct ctctgtgcgc
    10621 agtacttcct gtattattgt ggtggcggtc gatattcgca ctggcaaaaa aacgtgcttg
    10681 aatatttgtt gaaacccttt aacaaagcac aggaggcgtt gcgcgaacga tgaacattag
    10741 cgatgtagca aaaattaccg gcctgaccag caaagccatt cgcttctatg aagagaaggg
    10801 gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc acctacacgc agcagcatct
    10861 caacgaactg accttactgc gccaggcacg ccaggtgggc tttaacctgg aggagagcgg
    10921 cgaactggtg aatctgttta acgatccgca gcgtcacagc gccgacgtca aacgtcgtac
    10981 gctggagaaa gtagcggaga tcgaacagca tatcgaagaa ctgcaatcca tgcgtaacca
    11041 gctgctggcg ctggcgaatg cctgccccgg tgatgacagc gccgactgcc cgattatcga
    11101 aaatctctcc ggctgctgtc atcatcgggc ggggtgatta aggcttgcga aaatagtcgt
    11161 ccattatttt cgtcagctcc ggacgttccc cgtcatcatt gatggcaaaa tccagaaaaa
    11221 aagcatctgc ttgcggcgtt cggttttgcg ccatcaaccg gaaggtgagg gggcgataaa
    11281 tggcgtatgc cggatggctg ataacgaatc gggcaaagtc aataaaaagc ggcgagtgtg
    11341 tggaaaacag cgcctgcaaa ataatatcag ccttttcatc ctcccccaaa ccttgttgga
    11401 tcatgctctt aatacgagtt tcgctcaaat tcccggcctc aacaatttgc caggcttgat
    11461 ccagcgaggg gtaccgactg acgctctgct ttaactcttc tgaccaggtt tcggatgggc
    11521 tgatgtgctt atcagggccg aaactctccc atgcgctggg cggtaatgtt ttgcacgcgg
    11581 tgagttttgg tatgtggtat tgcagctctg ttttgtgcag acattccccg ccaggaaata
    11641 ccagggtttg ttgctcatca ttaaaatggg ttgccgtgct gagcaccagt gttttgagcg
    11701 gtcgcccttc cagcgcggcg gcagtagcct tatggtgtcc gtcgagcaga acgcagagat
    11761 aggaatcagg gagataccag gccagagcgc gggcgtccgg tttatcgcga taaaacgtcg
    11821 cacgctcagg attaaaatgt gatggcggct ggccgggtag aagaaagcag ggcgcggtgt
    11881 cactgaaaga gcaatattca ggatcccaga cggcaacggt tgccggatta ctggaactat
    11941 ggtccgttgc cgcccaaaag aaatgcccgt tgccatccgt cgggtaaagt tcacgatctt
    12001 ccagcaaata ataaccatca gccatgcatg agaacagcgg tgtcagcgcg gcaaaagcgt
    12061 ccgttatgcc agaccacggg cgattccagt tttcgagagc agacaaaaac cgggcggctc
    12121 cctgttcatc ctccgcaaaa ccgctgtaga caaattccgc acaggtaggg catccagcct
    12181 cggaccagaa acgtattaac gggacatcct gccagcagag cgaatggata agaccgtgcg
    12241 agtgcggatc gtaaatcaca cattgacgca gaaatccttt cccggacttc accgaaaaaa
    12301 tgacgcccgc agcggtattt ttcagcagac gtatttcagg ttttcgaaag gaggaaaaca
    12361 tggctactcc ttagccgaac attcaattaa cactatgtag ggggctatat gccggatgcg
    12421 gcgtaaagcc ttatccggca tgggttttgg tacagatttc caggtgtgat aagacgcatc
    12481 aggcgatgac cgcacggata atcagcgtta ttccttctat cgcaatcacc tcaacatgcg
    12541 taccagcgcc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    12601 gaccgcgccc gttgaccagc ggagattcca gaacaaaacg ttggccaatc aactgctgcc
    12661 cgcgctggtt taaatgactg tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    12721 tccaccacag ccaggcggcg agcagcgtca ggacggcaaa cattacccct tgccactccc
    12781 aacccagcgg cagcaaccag accaccagac cggtaatcac cgctgccacg ccgctccaca
    12841 acagataacc attgccgccc aacatctcgg ctgccagcag caaaccgccg agacttagcc
    12901 agaaaatatg ttgatggacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    12961 gtctttcacc agctcagcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    13021 catcatcacc actttgctgt tactggacga accgatctgc tgtaacgctt cggtgtattt
    13081 ctgtgcgacg aagtagttca ccgcctgaat atcgccggag gcgatggctt cagacaccat
    13141 tttggtggcg cgggcttctg cttcagcgga acgttcacgc gcttcagcct gtaaaaacgc
    13201 cgactgacgt tcgccttccg ctttcaggat ttgtgactgt ttttcacctt cggctttgag
    13261 gatttccgcc tgacggatcc cttccgcttc aaggatgtaa gcgcgtttgg tacgttccgc
    13321 tttcatctgc gcgttcattg aagagataag ctctgcgggc gggcgcacat cacgaatttc
    13381 aatacgggtg actttaatcc cccacgggtt ggtggcctca tcgacaatat gcagcagacg
    13441 cgagttaatg ctgtcgcgct gagagagcat ttcatcaagt tccattgaac ccagcacggt
    13501 acggatgtta gtcatagtca gattgatgat tgccagctcc agattgctga cttcataagc
    13561 cgcgcgcggc gcgtcaatca cctgaataaa gcacacggcg tcgatggtaa cgttggcgtt
    13621 atctttcgag ataacttcct gggaagggat gtcgagcact tgctccatca tattgatctt
    13681 gcgaccaatg cgatccataa acggcaccac caggcttaga cctggctgta acgttttggt
    13741 atagcgacca aagcgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    13801 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    13861 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    13921 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    13981 gaaaaaggga acagtgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    14041 tctggcgggt gatgcgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    14101 taagttaatt accggtcctt ctggttgtgg caaaagtaca ctgctaaaaa tagttgcttc
    14161 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    14221 gccagaaatc taccgtcagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    14281 tacggtatac gataacctga tttttccctg gcagatccgt aaccagcagc ctgacccggc
    14341 catttttctc gattttcttg aacgcttcgc tttaccggac agcattttga cgaagaatat
    14401 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    14461 gccgaaggtt ttattgctgg atgaaatcac cagtgcgctg gatgaaagta ataaacataa
    14521 cgttaatgag atgatccacc gttatgtacg cgagcaaaat attgctgtgc tgtgggtgac
    14581 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgctgg
    14641 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    14701 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    14761 aagatattct ctggagcgtc gggcgcgcga taatccagct gattattgtc ggctatgtgc
    14821 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    14881 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    14941 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtactg atcctctctg
    15001 ggtcgattga atttatcccg atgcaggtga tccctatcgc cgggatgata gccggcaacg
    15061 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    15121 aacagattca ggagaaactg agtcttggcg cgacgccgaa acaggcttca gcgatattga
    15181 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggtaggct
    15241 tagtgagttt acctggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    15301 cgattaaata tcagattatg gtgaccttta tgctgctctc aactgccagc ctgtctacca
    15361 ttattgcctg ctatttaacc tatcgtaagt tttacaattc gcgccaccaa ttggtggtga
    15421 cgcaattgaa gaagaaatga tgcaatgccg gatgcgcagc atcgcatccg gcgttgtggt
    15481 tcatgtgccg atcaacaaaa catcaataca acaacgcata cagctggcgg cgatacttcg
    15541 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaatgtt ttacgtgtct
    15601 gaccatctgc ggcggtgaga tctttacgca gatgcccaaa cagtaactcc agcgcctctt
    15661 cattgcgccc aacctggtgc agttgcagtg ccagttgcgt cgccagtgcg gcatcttctg
    15721 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca
    15781 gcagttcgat ttgcgccacc agcccctgat agcgggtgtc ctgatcctgc aacggaatgg
    15841 ttttcagcac ggcttctgca tcttcagaac ggttcagcgc aatcagcgtt tctgccaaca
    15901 acaggccgat ttccccgttc tgattcgaca actgccaggc gtctttcagc aatggcagag
    15961 cgtcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    16021 cacgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    16081 agccgtctac tggttgcccg ttctggaaca gatatacggt cggaatcgcg cgcagaccaa
    16141 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    16201 tgtactgcgc cgcgaggctt tccagaattg gggttaactg caaacagtgc tggctacgtt
    16261 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    16321 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    16381 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    16441 tcacgcgccc cggcagcagg cgcttaagca ccattaccgc ccaggtcacc agcgtcaccg
    16501 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    16561 ccggtcccaa cgtaaagcgg gcggcgatgc caggattttc gactggttta tcactttgcg
    16621 tctgattgac gttgtcggtg aagcgagtac gaatgggacc aggttcgatc aggctgactt
    16681 taattccgct gtggcgcagc tccatgcgca gtgcgtctga ccacgcctcc agcgcatatt
    16741 tactggccgc gtaagcgcca cgacccggtg tggagattaa tcccatcacc gatgaggtca
    16801 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    16861 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    16921 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    16981 gggcgatcac ttcgtcggct gcgcgatcaa cactttctgg cgaatccaga tcgatcaaca
    17041 cgccggtaaa tcccatgctg ttcatgcgct caacatcatc cggtttccgg caacctgcca
    17101 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaata ccactggaac
    17161 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    17221 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    17281 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    17341 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    17401 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    17461 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    17521 tgtggctgaa aaccacgcaa accgtcattg ccgcccagtt caaccagcac ccagcgcggc
    17581 tgatgctgtt tcagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcaccgctg
    17641 atgctggcat tgactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    17701 gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg
    17761 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    17821 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    17881 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    17941 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggctggat gacggcagca
    18001 gtggcgaagt gagactggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    18061 agttgcgcgc gaagcacgta ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    18121 acgcgctgga aaacgtcgag cttccggcgc tgctgcgcgg tgagagtagc gcagaaagtc
    18181 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctt gatcatcttc
    18241 cggcacagct ttctggcggc gaacagcaac gagtggcgct ggcgcgagcc tttaatggtc
    18301 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    18361 aaattgccga tctgcttttt tccctcaacc gtgagcatgg caccacgttg attatggtga
    18421 cccacgacct gcaactggcg gcacgctgtg accgctgctt acggctggtg aacgggcagt
    18481 tgcaggagga agtatgattg cacgttggtt ctggcgcgaa tggcgctcgc cgtcgctatt
    18541 aattgtctgg ctggcgctta gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    18601 cgatcgcatg gagaaaggtt taagccagca aagccgtgag tttatggcgg gcgatagggc
    18661 gttgcgcagt tcgcgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    18721 gaaagttggc aagcagctga ctttcgccac catgaccttt gcaggcgaca cgccgcagct
    18781 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    18841 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    18901 taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    18961 agtgattcag gaaccggatt ccggttttaa tcctttccag atagccccac gcctgatgat
    19021 gaatctggcg gatgttgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    19081 ttataaattc ggcggcagcg agaaccagct cgacggctat gagaaatggt tgttacccca
    19141 gcttaaacct gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    19201 gatagaacgt tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcggt
    19261 ggcggcggtt gcggtggcga tgaatcatta ctgtcgcagt cgctacgact tggtggcgat
    19321 cctcaagacg ctgggggcag ggcgagcgca actgcgtaag ttaatcgtcg gtcagtggtt
    19381 gatggtgctg gtgctttcag ccgttaccgg tggggcgata ggcctgttgt tcgaaaacgt
    19441 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    19501 gtggctgtgg gcgctcggca ccatgacggt tatttcgctg ctggtggggc tacgaccgta
    19561 ccgactgtta ctggccacgc agcctttacg cgtattacgt aatgatgtgg tagcgaacgt
    19621 ctggccgctg aagttttatc tgccgattgt caccgtggtg gttgtgctgc tgctcgccgg
    19681 actgatgggt ggcagtatgc tgctctgggc ggtgctggcg ggcgcggtag tactggcttt
    19741 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    19801 gctacctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    19861 gctttcggca ttttcgctct cctttatgct gctggcactg ttgctggtgt tgcgtggcga
    19921 tctgctcgac cgttggcaac aacaactgcc gccagaaagc ccgaactact ttttaatcaa
    19981 catcgctacg gaacaggtta cgccgctgaa agcgtttctc gcggaacatc agatagtccc
    20041 ggaatcgttt tatccggtgg tgcgggcgcg gttgacggcg attaacgaca agccgacaga
    20101 aggcaatgga gatgaggcgc tcaaccgcga gctgaatctc acctggcaaa atacgcggcc
    20161 tgatcataac ccgatcgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    20221 agaggggctg gcgaaacgct taaacgttgc cctcggcgat accgtgactt ttatgggcga
    20281 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    20341 gcctaatttc tattttattt tcccggaagg ggcattagac gggcaaccgc agagctggct
    20401 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    20461 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    20521 ggtaagtcgg gcgctggaag tgatggtggt gctggtcacc gcctgcggca tgttgctgtt
    20581 gctggcgcag gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac
    20641 acttggtgcg gggaaaaaat tgctgcgcac gacattgtgg tgtgagttcg ccatgctcgg
    20701 gtttgtatcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcgg tactgcaatc
    20761 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgtgc tgccgtgcag
    20821 cggtgcgcta ctgctgtcgc tttgcggcgg ctggctgggt gcgcgactgc ttaagggtaa
    20881 ggcgctgttc aggcagtttg cggggtgatg acaggtgtta gattgcctga tgcgctacgc
    20941 ttatcaggcc tacgaagatc gtgcaatata ttgagttttc gaaattttgt aggccggata
    21001 aggcgttcac gccgcatccg gcagttgtgc gccgatgcca gatgcgacgc aggcgcgtct
    21061 tatcatgcct acgaaacagc acttacctgt aggccggata aggcgttcac gccgcatccg
    21121 gcatgaacag cgtgcacaga atcccccatc actgcgcctt aacccattcc gccacttccg
    21181 cccactcacc gcgaaagaca actttttccg cttttttttc aagctgatag cgatacatcg
    21241 ggtcgtaata ttcttcaagc agcggcacta gccaggccag atgaccgtcg gtgctgccgg
    21301 tggtgagttg cgttgtcagc gccgcatcca gccttgcagc cagttcgtta tagcgttgta
    21361 gtcccagccg acgtttaatc gccgaaagcc cgtgatgcag gtattcgcaa tactcctgcc
    21421 agccctgttc gtcgccataa gcgtgggtaa aatcatgatg catacgcaag aaatactctt
    21481 cgttcaggcg ttcaagacgg atctcaaacg gatcttctac caccgcaatc gtcgcctgag
    21541 tcattcgttc gcgcagacat tccggcaggt gattcgaacc gatcatccgg ctttcatctt
    21601 ccagcaccca caagcgcaaa ttctgacggg cgtcggtttt tagcatttcg gcagccagca
    21661 ggttttcaaa actcgcctgg ctaagttgtg gttgtaacgt gcgaccaaaa gcagaaccgc
    21721 gatggcgcgc cagcccttcc agatcaacac cgttcggttg ttgttgcact aacagcgttt
    21781 tgccgcagcc ggtacaaccg ccaatcagca ctatcggttt ttgtgacagt tcaatggtcg
    21841 cctgaatcgc ggtctggcgc agtgccttat aaccgccttc caccagcgga taatcaatcc
    21901 ccgcgtcatg cagccagcgt tgcacaatat gcgagcgctg accgccacgg gcgcagcaga
    21961 gaataccgtg cggattttgc aggcacgctg cccgccaggc gtctatacgc tgctgacgaa
    22021 tttcacccgc caccagctta tgccccagtg ccagcgccgc gtctgaccct tgctgtttat
    22081 agcagatgcc aacggcggcg cgttcatcgt tattcattaa cggtaggttg atagcggcgg
    22141 gcattgcgcc ttgctcaaac tcgatggggg cgcgaacatc aataatgggc gtatcagcaa
    22201 tcagcagggc acgatagtcc tgttccgtgt gtctctcttg catagtaaaa agtgaacctc
    22261 aaatcagctt gcgcgctatt ttacgcgcca acgcgcaagg aaacttgatt tttaactgcg
    22321 tgggttgccg aaaatttcta aaaatccgct gatttccggc ctgcgctggg taaacaaggt
    22381 cacaatatct tctaccgctt tgccgctgcc aaatttgcgc catgccagac tcaatggcga
    22441 aggagggcgc atcgttggga ttacccggct gaccagttgt tgattatcga tcattgactg
    22501 gcaaagtgat tttggcaaaa aaccaatgcc aacgcccgcc agatgggcgg cgattttcgt
    22561 ttccatatcg ggaacaataa tctctttttg ccccggcaat cgccaggcga cgcgtttggt
    22621 taaggtgcgg gcgctgtctt caatattgac cgccggaaag cgccgcaact gtgcttctgt
    22681 tagcggctct tcaacgttcg ccagcggatg ctccgccgcc atgacaaagc gccattgcac
    22741 cgatcctaag ggatcaagac taaaggtatt tgccagcgcc tcggttcccg tgacgccgat
    22801 agccagtgaa aaaccttcgt acaatagcga gtcccagacg cccatataga tttgtcggga
    22861 gatgtgaaat tgggtaaacg ggtaacgttc atttagccac gccagcagcc tggcaacggc
    22921 ctgggggtta tagagcaggt tgttgatgac gatattcacc tggcgttcca cgccatcatt
    22981 cacctgttgt aactcgctgg gcatactttc cagccagctc agccagtctc tggcctgaca
    23041 aagtagatgc tcgccagccg ccgtcaacgt cacgctgcga gtcgtgcgga aaaacagcgc
    23101 cactccggta ttctcttcca gaagtttaat gcgataactg atcgtcgccg tggttttaca
    23161 taatcgttct gccgcttttg aaaaacttcc tgtttcagca accgcaatga aagtccgcaa
    23221 ggtttctgga tcgaacatct tcaggtatcc ccttttaaat ccgcaagttg cgtgattttc
    23281 ttatcctctg atttatcagt atttttacat gataaccctg ttcaatttgt ggactaaatc
    23341 tagttttgga aaaatattcc aacttttgta ttgatgttgt tctcttaagg ttttagattg
    23401 cctgttattg aaaccaagct gaccggtcgg cggtggttga acggaattat gttacaagga
    23461 caaaaagatg aaacttcagg tattaccgtt aagtcaggaa gcctttagtg cttatggcga
    23521 cgtaatcgaa acgcagaaac gggatttttt ccatattaac aatggactgg tggagcgtta
    23581 ccacgatttg gcgctggttg agattcttga gcaagaccgt acgcttatca gcattaaccg
    23641 cgcgcaaccg gcgaatctgc cgctgactat tcacgaactc gaacgtcatc cgctgggtac
    23701 acaggcgttt atcccgatga aaggtgaagt gtttgtggtg gtggtggcgt taggtgacga
    23761 caaaccagac ctgtcaaccc tgcgggcgtt tattaccaat ggcgaacagg gagtgaatta
    23821 ccatcgtaac gtctggcatc acccactttt cgcctggcag cgcgtcaccg attttctgac
    23881 catcgatcgc ggcggcagtg acaactgtga tgttgaaagt attcctgaac aggaactctg
    23941 ttttgcgtga cgcctgcaac cgacttgcat aagataaacg aattgttcat tgtttatgct
    24001 catttgtagg tcggagttaa cgtaggtatg acggaagtta gacggcgcgg caggccagga
    24061 caggcggagc ctgtggcaca gaagggtgca caggcgttag agcggggaat tgcgattctg
    24121 caatatctgg aaaaaagtgg gggaagttcg tcggttagcg atatctctct caatctggat
    24181 ttgccgctct ccacgacctt tcgcttgctg aaggttttac aggcagcgga ttttgtctat
    24241 caggacagtc agttaggctg gtggcatata ggattaggtg tctttaacgt cggtgcagcg
    24301 tacatccata accgcgatgt actctccgtc gccgggccgt ttatgcgccg cctgatgtta
    24361 ctttccggcg aaacggtcaa tgtcgcgatc cgtaacggca atgaagcggt attaattggt
    24421 cagttagagt gtaaatcgat ggtcaggatg tgtgcgccgc tgggcagtcg tctgccgctg
    24481 catgcttccg gagcgggcaa ggcgctgctt tatccgctgg cggaagaaga gttgatgagc
    24541 atcattctgc aaaccggttt gcagcagttt acgccaacga cgcttgtgga tatgcccacc
    24601 ttgctgaagg acctggaaca agcgcgtgaa ctgggctata ccgtagataa agaagagcat
    24661 gttgtcggcc tgaattgtat agcttcagca atttacgatg atgtaggtag tgttgttgcc
    24721 gctatctcca tctccgggcc ttcatcaaga ctgacagaag atcgttttgt cagccagggt
    24781 gagctggtca gagacaccgc ccgcgatatc agcacggcgt tgggactgaa agcacatcca
    24841 tagtgtctgt cgtatcccgc tctgcggagc gggttttttt gacaaaattt gagagttgga
    24901 aaatttttcc aattaataga ggtaggaata aaatggcaaa aatgagagcc gttgacgcgg
    24961 caatgtatgt gctggagaaa gaaggtatca ccaccgcctt cggcgttccg ggagctgcaa
    25021 tcaatccgtt ctactcagcg atgcgtaagc acggcggtat tcgtcacatt ctggcgcgtc
    25081 atgtggaagg tgcttcgcac atggcggaag gttatacccg cgcaacggca gggaatatcg
    25141 gcgtatgtct ggggacttcc ggtcctgcgg gcacggacat gatcaccgcg ctctattccg
    25201 cttctgctga ttccattcct attctgtgta ttaccggcca ggctccgcgc gcccgtctgc
    25261 ataaagaaga tttccaggcc gttgatattg aagcaattgc taaaccggtc agcaaaatgg
    25321 cggttacagt tcgtgaagcg gcgctggtgc ctcgtgtgct gcaacaggcg tttcacctga
    25381 tgcgttctgg tcgcccgggt ccggtactgg tggatttacc gttcgacgta caggttgcgg
    25441 caatcgagtt tgatcctgat atgtacgaac cgctgccggt ctacaaacct gctgccagcc
    25501 gtatgcagat cgaaaaagct gtagaaatgt taatccaggc cgaacgtccg gtgattgttg
    25561 ccggaggcgg ggtaatcaat gctgacgcag ctgcactgtt acaacagttt gctgaactga
    25621 ccagcgttcc ggtgatccca acgctgatgg gctggggctg tatcccggac gatcatgaac
    25681 tgatggccgg gatggtgggc ctgcaaaccg cgcatcgtta cggtaatgca acgttgctgg
    25741 cgtccgacat ggtgtttggt atcggtaacc gttttgctaa ccgtcatacc ggttcggtag
    25801 agaaatacac cgaagggcgc aaaatcgttc atatcgatat tgagccgacg caaattggcc
    25861 gcgtactgtg tccggatctt ggcattgtct ctgatgctaa agcggcgctg acgctgctgg
    25921 ttgaagtggc gcaggagatg caaaaagcgg gtcgtctgcc gtgtcgtaaa gagtgggtcg
    25981 ccgactgcca gcagcgcaaa cgcactttgc tgcgcaaaac ccacttcgac aacgtgccgg
    26041 tgaaaccgca gcgcgtgtat gaagagatga acaaagcctt tggccgcgat gtttgttatg
    26101 tcaccaccat tggcctgtca caaatcgctg cggcacaaat gctgcatgtc tttaaagacc
    26161 gccactggat caactgtggg caggcaggcc cgctgggctg gacgattccg gcggcgctgg
    26221 gggtttgtgc cgctgatcca gagcgcaatg tggtggcgat ttccggtgac ttcgacttcc
    26281 agttcctgat tgaagaatta gccgttggcg cacagttcaa cattccgtac atccatgtac
    26341 tggtcaataa cgcctatctg ggtctgattc gccagtcgca acgtgccttt gatatggatt
    26401 actgcgtaca gctcgcgttc gaaaatatta actccagcga agtgaacggt tatggcgtcg
    26461 accacgtaaa agtagcggaa ggtttaggtt gtaaagcaat tcgcgttttc aaaccggaag
    26521 atattgcacc agcctttgaa caggcaaaag tcttaatggc gcaatatcgg gtaccggtag
    26581 tcgtggaagt tattctcgag cgtgtgacta atatttcgat gggcagtgaa ctggataacg
    26641 tcatggaatt tgaagatatc gccgataacg cagcggatgc accgactgaa acctgtttca
    26701 tgcactatga ataagggaga taaataatgt tacgtttctc tgctaattta tcgatgttat
    26761 ttggtgaata tgattttctc gcccgttttg agaaagctgc gcagtgtggt tttcgcggcg
    26821 ttgaatttat gttcccttat gactacgaca ttgaagaatt aaaacaggtg ctggcgagta
    26881 ataaactcga gcatacgctg cacaatttac cagcgggtga ctgggcggcg ggcgagcggg
    26941 gtattgcctg tattcctggc cgtgaagaag agtttcggga tggcgtagca gcagcgattc
    27001 gttatgctcg tgctctgggt aataaaaaaa ttaactgtct ggtcggtaaa acgccgactg
    27061 gtttcagcag tgaacagatt cacacaacgc ttgtagaaaa cctgcgttat gccgcgaata
    27121 tgctgatgaa agaagatatt ttattactga ttgaacctat taaccatttt gatattcctg
    27181 gtttccatct caccggaacc cggcaggcac tgaaactgat tgacgatgtt ggttgctgca
    27241 atttgaaaat tcagtatgac atttatcaca tgcagcggat ggaaggtgaa ttaactaata
    27301 ccatgactca gtgggccgat aaaattggtc acctgcaaat tgctgataat ccgcatcgcg
    27361 gcgaaccggg aaccggagaa attaattacg aatatctctt tacggtaatt gaaaattctg
    27421 actacaacgg ttgggttggc tgtgaatata aaccccaaac caccacggaa gccggtttac
    27481 gctggatgga tccgtaccgt taaaacgtaa cgctattcag acaatgcttt tttaggccgc
    27541 taagttggca ggggatcgtg ttgtctgaat tcaggaaaag cgaaatttaa aagaggttaa
    27601 ttatgacact gggatttatt ggcttaggca ttatgggtac accgatggcc attaatctgg
    27661 cgcgtgccgg tcatcaactg catgtcacaa ccattggtcc ggttgctgat gaattactgt
    27721 cactggacgc cgttagtgtt gaaactgcgc gccaggtaac agatgcatcg gacatcattt
    27781 ttattatggt gccggatacg ccacaggttg aagacgttct gtttggtgaa aatggttgta
    27841 ccaaagcctc gctgaagggc aaaaccattg ttgatatgag ctccatttcc ccgattgaaa
    27901 ctaaacgttt cgctcgtcag gtgaatgaac tgggcggcga ttatctcgat gcgccagtct
    27961 ccggcggtga aatcggcgcg cgtgaaggga cgttatcgat tatggttggc ggtgatgaag
    28021 cagtatttga acgtgtaaaa ccgctgtttg aactgctcgg taaaaatatc actctcgtgg
    28081 gcggtaacgg cgacggtcaa acctgcaaag tggcaaatca gattatcgtg gcgctcaata
    28141 ttgaagcggt ttctgaagcc ttgttgtttg cttcaaaagc cggtgcggac ccggtacgtg
    28201 tgcgccaggc gctgatgggc ggctttgctt cctcacgtat tctggaagtt cacggtgagc
    28261 gcatgattaa acgcaccttt aatccaggtt tcaaaattgc gctgcaccag aaagatctca
    28321 acctggcact gcaaagtgcg aaagcactgg cgctgaacct gccaaacacc gcgacctgcc
    28381 aggaattgtt taatacctgt gcggcaaacg gtggcagcca gttggatcac tctgcgttag
    28441 tgcaggcgct ggaattaatg gctaaccata aactggcctg atacccgcaa taaaaatggc
    28501 cgatatcaga aaatgaatcg gccagcaata ttaaaaaaag aaagcagcca aagatgttgc
    28561 ttcagtatta aaaataa
//
LOCUS       DADTFG010000056        28422 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.056, whole genome shotgun sequence.
ACCESSION   DADTFG010000056 DADTFG010000000
VERSION     DADTFG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.056"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            153..2003
                     /locus_tag="J1138_002173"
     CDS             153..2003
                     /locus_tag="J1138_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA8453972.1"
                     /translation="MATIIPTISSCNDKITAGEKRLARILERGLGEECCCWYDIPTGD
                     KHLHPDFMILSPEKGIIFLEVKDWFITKIKKANKSNVLYETQNGIQSLKNPIEQVRRY
                     AFEAVNQLKKDPLLCQQDDRYNGNLLFPYGYGVYFSNITREALNNKFTQEELLGIFPS
                     DLIICKDEINEFMSKEDVSSKIHSLIKYDFKCHATQEQLNRVRWHLYPDVRIEREKRS
                     KNRDEFSVDAPTIISILDTQQEQLARSMRDGHRIIHGVAGSGKTLILYHRCQELAKKN
                     DSEKPILVICYNITLAKKLRSMFINHPFAEKIKVKNFHAWCFQQIKENKITIPEGDNI
                     FENMELALTDGFKTGKIKPEQYSAVLIDEGHDFKPEWLKILAKMTDSQDENLLFLYDD
                     AQSIYQKKKALDFTLSSVDIKATGRTTILNINYRNTQQILHFASCIAFNYLNSHIDSS
                     LTYHKPAAGGMNGDYPNLEHFDTQDEEIARAVEWITEQNEQGIPWSEIAILSPSTHTL
                     SNSLSSVLESKNIPFNLIVSSADKKSWTPEQERISVMPLPSSKGLEFHSVVIIDAART
                     RDNSDDLSEDIKRLYVGFTRARCNLLVTLHGEGALNEHLLKTYEQSPQYM"
     gene            2148..2441
                     /locus_tag="J1138_002174"
     CDS             2148..2441
                     /locus_tag="J1138_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HBA8453973.1"
                     /translation="MLLNSSALATGINKFKLPATKFQLLQKLLAKEMKSVVDNTSKYP
                     DWSKRDDIKAKPKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            2656..2994
                     /gene="symE"
                     /locus_tag="J1138_002175"
     CDS             2656..2994
                     /gene="symE"
                     /locus_tag="J1138_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBA8453974.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVISSKTPR"
     gene            complement(3036..3200)
                     /locus_tag="J1138_002176"
                     /pseudo
     CDS             complement(3036..3200)
                     /locus_tag="J1138_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
     gene            3378..3548
                     /locus_tag="J1138_002177"
     CDS             3378..3548
                     /locus_tag="J1138_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA8453975.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSTVQQA
                     YQTLETMNLITP"
     gene            complement(3678..4598)
                     /locus_tag="J1138_002178"
     CDS             complement(3678..4598)
                     /locus_tag="J1138_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBA8453976.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLLLQNGADPEW
                     IQKITGLSAEQMQALEQPLPDREHDSWLES"
     gene            4783..6081
                     /locus_tag="J1138_002179"
     CDS             4783..6081
                     /locus_tag="J1138_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HBA8453977.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALILESMTRNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATLALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRIAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNLYKSMKN"
     gene            6179..7330
                     /locus_tag="J1138_002180"
     CDS             6179..7330
                     /locus_tag="J1138_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HBA8453978.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            7340..8107
                     /locus_tag="J1138_002181"
     CDS             7340..8107
                     /locus_tag="J1138_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453979.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            8104..8361
                     /locus_tag="J1138_002182"
     CDS             8104..8361
                     /locus_tag="J1138_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBA8453980.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            8426..9286
                     /locus_tag="J1138_002183"
     CDS             8426..9286
                     /locus_tag="J1138_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HBA8453981.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFYQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLAKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            9354..10532
                     /locus_tag="J1138_002184"
     CDS             9354..10532
                     /locus_tag="J1138_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8453982.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPIMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMSLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLMLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTAQLFKLQVVTS
                     "
     gene            complement(10545..11099)
                     /gene="kptA"
                     /locus_tag="J1138_002185"
     CDS             complement(10545..11099)
                     /gene="kptA"
                     /locus_tag="J1138_002185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBA8453983.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGTCIRAVQGHSTSQVVIAFIEKTPPQF
                     LYHGTASRFLEAIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            11349..12032
                     /locus_tag="J1138_002186"
     CDS             11349..12032
                     /locus_tag="J1138_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBA8453984.1"
                     /translation="MTQQGDAVAGELATEKVGVKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            12029..12490
                     /locus_tag="J1138_002187"
     CDS             12029..12490
                     /locus_tag="J1138_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8453985.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            12503..13675
                     /gene="iadA"
                     /locus_tag="J1138_002188"
     CDS             12503..13675
                     /gene="iadA"
                     /locus_tag="J1138_002188"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBA8453986.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGITSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13740..14651
                     /gene="hypT"
                     /locus_tag="J1138_002189"
                     /gene_synonym="qseD"
     CDS             13740..14651
                     /gene="hypT"
                     /locus_tag="J1138_002189"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBA8453987.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIEPG"
     gene            14658..15029
                     /locus_tag="J1138_002190"
                     /pseudo
     CDS             14658..15029
                     /locus_tag="J1138_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(15077..15460)
                     /gene="iraD"
                     /locus_tag="J1138_002191"
     CDS             complement(15077..15460)
                     /gene="iraD"
                     /locus_tag="J1138_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBA8453988.1"
                     /translation="MMRQSVQTVLPESTGNNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
     gene            complement(15874..16647)
                     /gene="uxuR"
                     /locus_tag="J1138_002192"
     CDS             complement(15874..16647)
                     /gene="uxuR"
                     /locus_tag="J1138_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBA8453989.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNADSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(16862..18322)
                     /locus_tag="J1138_002193"
     CDS             complement(16862..18322)
                     /locus_tag="J1138_002193"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBA8453990.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(18403..19587)
                     /gene="uxuA"
                     /locus_tag="J1138_002194"
     CDS             complement(18403..19587)
                     /gene="uxuA"
                     /locus_tag="J1138_002194"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBA8453991.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQATE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            19927..21270
                     /gene="gntP"
                     /locus_tag="J1138_002195"
     CDS             19927..21270
                     /gene="gntP"
                     /locus_tag="J1138_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBA8453992.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQI
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(21512..22414)
                     /gene="fimH"
                     /locus_tag="J1138_002196"
     CDS             complement(21512..22414)
                     /gene="fimH"
                     /locus_tag="J1138_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBA8453993.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSSFSGTVKYNGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(22434..22937)
                     /gene="fimG"
                     /locus_tag="J1138_002197"
     CDS             complement(22434..22937)
                     /gene="fimG"
                     /locus_tag="J1138_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBA8453994.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGRVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(22950..23480)
                     /gene="fimF"
                     /locus_tag="J1138_002198"
     CDS             complement(22950..23480)
                     /gene="fimF"
                     /locus_tag="J1138_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBA8453995.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNEIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(23490..26126)
                     /gene="fimD"
                     /locus_tag="J1138_002199"
     CDS             complement(23490..26126)
                     /gene="fimD"
                     /locus_tag="J1138_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HBA8453996.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(26193..26918)
                     /gene="fimC"
                     /locus_tag="J1138_002200"
     CDS             complement(26193..26918)
                     /gene="fimC"
                     /locus_tag="J1138_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBA8453997.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(26955..27494)
                     /locus_tag="J1138_002201"
     CDS             complement(26955..27494)
                     /locus_tag="J1138_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA8453998.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAEACR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(27559..28107)
                     /locus_tag="J1138_002202"
     CDS             complement(27559..28107)
                     /locus_tag="J1138_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8453999.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLDQDGATSSAVGFNIQLNDCDTDVASKAAVAFLGTTID
                     TNHTNVLALQSSAAGSATNVGVQILDKTGTALALDGASFSAQTTLNNGTNTIPFQARY
                     YAIGAATPGAANADATFKVQYQ"
BASE COUNT         7004 a         7197 c         6920 g         7301 t
ORIGIN      
        1 atagacgtcc tattgaggcg taatagatgt attaatttcc aaatatgcaa acattctggt
       61 acagtccaaa aaagaaaaat catcaaccaa aacaatcagc aaataaatta acaacgattg
      121 aatcataatc aacacgaata caagagatta ccatggcaac aataatccca acaatcagta
      181 gctgcaacga taaaattact gccggagaga aaaggctggc aagaattctg gaaagaggac
      241 taggcgaaga gtgctgctgt tggtatgaca ttcccacggg tgataagcat cttcacccag
      301 attttatgat actttcgcca gaaaaaggca ttatttttct cgaggtaaaa gattggttta
      361 ttacaaaaat aaaaaaagcc aacaagtcta atgtgttgta tgagacacaa aatggtattc
      421 aatctttaaa gaatccgata gaacaagtta ggcgatatgc tttcgaagct gtaaaccaat
      481 taaaaaaaga ccccctattg tgccaacagg atgatcgtta caatggcaat cttcttttcc
      541 cctatggata tggcgtctac tttagtaata ttacacgaga agcattaaat aataaattta
      601 ctcaagaaga gttgctcggg atcttcccat cagatctcat catctgcaaa gacgaaataa
      661 atgaattcat gtcaaaagag gatgtttcat ccaaaataca ttcgttgatt aaatatgact
      721 tcaaatgtca tgctacacaa gaacagttga acagggttag atggcactta taccccgatg
      781 tcagaataga aagggaaaag agaagtaaaa accgtgatga atttagtgtc gatgcaccaa
      841 ccatcatttc tatcctggat actcaacagg aacaacttgc cagaagtatg agagatggcc
      901 accggatcat tcatggtgtt gcaggttcag ggaagacatt gatcctctat catcgctgcc
      961 aggaattagc gaaaaaaaat gacagcgaaa agccaatact tgtcatttgc tacaatatta
     1021 ctttagccaa aaaacttcgg tcgatgttca tcaatcatcc ctttgcagag aaaataaagg
     1081 ttaaaaactt tcatgcttgg tgtttccaac aaattaaaga aaacaagatc actattccag
     1141 aaggggataa tatttttgaa aatatggagc tagcgttgac tgatggtttc aagacaggaa
     1201 aaataaaacc agaacaatac agcgctgtat taattgatga aggacatgat tttaagcccg
     1261 aatggttaaa aatcctggca aaaatgactg actctcagga cgagaatttg ctattccttt
     1321 atgatgatgc tcagtcaatt taccaaaaaa agaaagcatt agactttaca ctgtccagcg
     1381 tagatattaa agcgacagga cgcacaacca ttcttaatat aaattataga aacacacagc
     1441 aaatcctaca ctttgccagt tgcattgcat ttaattatct taatagccat atcgatagtt
     1501 ctttaaccta tcataaacct gctgcgggag gaatgaatgg cgattatccc aaccttgaac
     1561 atttcgacac acaggacgaa gaaatagcaa gagcagtcga atggattaca gagcaaaatg
     1621 aacaagggat tccgtggtct gaaattgcta tactaagtcc atcaactcac accctttcta
     1681 actctctaag ttctgtactt gaatcgaaaa acatcccctt taatcttatt gtaagctctg
     1741 cagataaaaa gtcctggacg ccagaacaag agcgtatttc tgttatgcca ttacccagca
     1801 gcaaaggact tgagtttcac tcagttgtca ttattgatgc agcgagaaca cgggataata
     1861 gcgatgattt gagtgaggat ataaaaagac tctatgtagg ttttacccga gcacgttgca
     1921 atctcttggt aaccttgcac ggtgaaggtg cgttaaatga gcatctacta aaaacctatg
     1981 agcaaagccc acaatatatg taaaaatgat aaattatctc cataacgccc ggtaacgctg
     2041 taccgggcgt tatgaatgta tatctaagat attagtcacg cctgatagcc gctaccagaa
     2101 acgccatcat ctccctctca tctttgctgt gataaaagcg taccattttg ctgttaaatt
     2161 ccagcgcttt agccactgga attaacaagt tcaaacttcc ggcaacgaag ttccagttat
     2221 tacaaaaatt actggcaaaa gagatgaaaa gcgtggttga caacacatcg aaatatcctg
     2281 actggagtaa acgcgatgat attaaagcga aaccgaaagt tgaacttatt ctgcttctac
     2341 acaagcataa gttcccgcca gtagcgaatg atgatgttta tatgggtgtg ctggcacagg
     2401 cagagaattt taaagaacat catatgagtg ctttaaatta attatgaagg ccggatacac
     2461 tttgttatcc ggccattctg aattaacgtt aatttctctt ccagatcttt ccagtaacgc
     2521 ttttattatt ttctattttt agctggactt tccctgtatt tactgatgat atatccaggt
     2581 atttagcgcg gtgcgaatgt gcgccaacac acccgcaccg ctcatcacaa tccctatttc
     2641 acgagaatag taatcatgac tgacacgcat tctattgcac aaccgttcga agcagaagtc
     2701 tccccggcaa ataaccgtca attaaccgtg agttatgcga gtcgctaccc ggattacagc
     2761 cgtattcccg ccatcaccct gaaaggtcag tggcttgaag ccgccggttt tgccaccggc
     2821 acggcggtag atgtcaaagt gatggaaggc tgtattgtcc tcaccgccca accacccgcc
     2881 gccgaagaga gcgaactgat gcagtcactg cgccaggtgt gcaagctgtc ggcgcgtaaa
     2941 caaaagcagg tgcaggattt tattggggtg attagtagca aaacgccacg ctaagtttct
     3001 ctccccttcc ccatacggga aggggaattg ccctctcact ccacatcctc cctgcgtaac
     3061 ccaatatccc tcaaccgctc atcactcatc tgctgtaaca cccgtcgggc ctgcatttgc
     3121 cgccaccagc tcctcaccgc ttgccagagt tgtacgaaac cgataaacgg cgctttggct
     3181 ttgttttagt gaaattccat ctgctgcctt cctcatcttt cagaggcttc atcatccgtc
     3241 ttctggcagt tcacaataca gatgcaaata ttagttttat ttaacataca gacaggttaa
     3301 aacagcatct gcatccggtt ttttagcctc atctgtactg gtttttctct ctgtatggtc
     3361 caaataagga atacagcatg acgcgttatc aacatctggc aactctgctt gccgagcgga
     3421 ttgagcaagg gctgtatcgt cacggggaga aattgccgtc ggtgcgtagc ttgagtcagg
     3481 agcacggcgt cagcaccgtg cagcaggcgt atcagacgct ggagacgatg aacctcatca
     3541 ctccgtagcc gcgttcgggt tattttgtcg cgcaacgtaa agcccagccg ccagtaccgt
     3601 cgatgacgcg tccggtggaa attacccagt gggatcaggt gctggatatg ctggaggcac
     3661 atagcgatag ttccatatta gctctcgagc catgaatcgt gctcacggtc aggcaagggc
     3721 tgctctaatg cctgcatttg ctctgccgaa agtccggtaa tcttttgtat ccattcagga
     3781 tctgccccat tctgcaacaa caatcgaaga atgcgctgtt ccccttttat atatccatca
     3841 ttatgaattc gctctgcaat cgtcattatt cgctcccttt gttgtggcag tcgacgggta
     3901 atttcactga taaactcatt aaaacgcgct tcatcaccag tcagtaaaat gtaatttaac
     3961 agcgcagtta tctggctgtc attagcacac tctgtaacca gtaatactac cagttggtca
     4021 ataagcccca tcagatcgcg ctggcgaata tgcttttgga tcaactccaa cagggcaact
     4081 ctgcgatgct gcacaatctc gtcgtctggc acgacagtga tatccaccag cgggaacgcg
     4141 gcggtataaa gcttccgtgc ggtagtcggg tcggcaaatt cgtccagcca gcacagggac
     4201 cagggataag gactacggct accgtgataa aacagcatcg ggatgaccag cggtagctgc
     4261 cggcgtttat catgttcgat atggcgctgc atcaccgcca tggaatatcg cattaggcga
     4321 aaggccatat gaatatcctc acggctctga tgttcaatca ctacataaat atagccatcg
     4381 ccttcacggg tctttaccga ccacagaata tcggaatgta gcgcccgcaa tttttcatcg
     4441 acaaagctgg cggattccag ttttaagcta tcgagatcgc acagttcacg taaatctttg
     4501 ggtaagtgga tctccatgaa atcccgcgcg gtgtcagagt gtgtgagaaa ggatttaaat
     4561 aatgcgtcat gcggcgtgct ggtcgtaaag tttgtcatgg cgatccgtca cctgtagata
     4621 agatggccca actttagcga ctaacaaagc cattcacacc tggctatttc ctattccccg
     4681 aggcggtttc caggctattt ttattcttct gcaaacgata catttttatg gaagatatct
     4741 cgctttttgc gttttcttac cgtcaggctc tattcttacg atatgaataa actcattgaa
     4801 ctcagacgcg ccaaaatgtt ggcgctctct ttactgctta tcgccgctgc tacctttgtc
     4861 gttacgctgt ttttgccgcc caatttttgg gtgagcggtg tgaaggcgat tgctgaagcg
     4921 gcgatggtcg gcgcgctggc ggactggttt gcggtggtgg cgctgtttcg ccgcgtgccg
     4981 attccgatca tttctcgtca tacggcgatt atcccgcgta ataaagaccg gattggcgaa
     5041 aatctcggcc agttcgtgca ggaaaaattt ctcgataccc aatcgctggt ggcattgatt
     5101 cgacgtcacg aaccggcgtt gttgattggc aactggttta gtcagccgga gaacgcccgc
     5161 cgcgttggtc agcatctgtt acagatcatg agcggttttc tcgaactgac cgatgatgcg
     5221 cgtattcagc gcctgcttaa gcgcgcggtc caccgggcga tcgataaggt cgatctttcc
     5281 ggcaccagcg cactgattct ggagagcatg accagaaacg atcgtcatca ggtgctgctc
     5341 gatacgctga tcgcacagtt gatcgccctt ctccagcgcg ataaatcgcg caagtttatc
     5401 gcccagcaaa ttgttcgctg gctggagagc gagcatccac tgaaagccaa aattttgccc
     5461 actgaatggc tgggcgaaca tagcgcggag ttggtttctg acgcggtgaa ttctttgctt
     5521 gatgatatca gccgcgatcg tgcgcatcag atccgccatg cgtttgatcg cgccactctt
     5581 gccctgatcg acaagctgaa aaacgatccg gaaatggcag cgcgagccga tgccgtaaaa
     5641 agctatctga aagaagatga agcttttaac cgctatctca gtgaattgtg gggggattta
     5701 cgggaatggc tgaaagcgga tatcaacagt gaagattctc gtgtgaaaga acgtatcgcg
     5761 cgggcgggtc aatggtttgg cgaaacgtta attgccgatg atgccttgcg ggcgtcgtta
     5821 aatggtcatc tggaacaagc cgcacaccgc attgcgcctg agttttccgc attcctgact
     5881 cgccatatca gcgacacggt aaaaagctgg gacgcacggg atatgtcgcg gcaaatcgag
     5941 ttaaatatcg gcaaagatct ccagtttatc cgtgtcaacg gtacgctggt tggcggttgt
     6001 attggactta ttttgtattt gctgtcgcag ctcccagcct tgttccccct cggcaattta
     6061 tataaatcca tgaaaaacta agtggctaac cgcgagagag atcaaataaa ccacactaaa
     6121 agaatggtaa taatgtggcc tcttttcatt atctcccgtg gtacggggaa ggaaaatcat
     6181 gtcacttgtc accgatctac ccgccatttt cgatcagttc tctgaagctc gccagaaagg
     6241 ctttctcacc gtcatggatc tcaaggagcg cggcattccg ctggttggca cctactgcac
     6301 ctttatgccg caagagatcc cgatggcagc cggtgcggtt gtggtttcgc tatgttccac
     6361 ctctgatgaa accattgaag aagccgagaa agatctgccg cgcaacctct gcccgctgat
     6421 taaaagcagc tacggcttcg gcaaaaccga taaatgcccc tacttctact tttctgatct
     6481 ggtggtcggt gaaaccacct gcgacggcaa aaagaaaatg tatgaataca tggcggagtt
     6541 taagcccgtt catgtgatgc aactgccaaa cagcgttaag gacgatgcct cgcgtgcatt
     6601 atggaaagcc gagatgctgc gcttgcaaaa aacggtagaa gaacgttttg ggcacgagat
     6661 tagcgaagat gctctgcgcg atgccattgc gctgaaaaac cgcgaacgtc gcgcactggc
     6721 caacttttat catttggggc agttaaatcc tccggcgctt agcggcagcg acattctgaa
     6781 agtagtctac ggcgcaacct tccggttcga taaagaggcg ttgatcaatg aactggatgc
     6841 aatgaccgcc cgcgttcgtc agcagtggga agaaggccag cgactggacc cgcgcccacg
     6901 tattttaatc accggctgcc caattggcgg cgcagcagag aaagtggtgc gcgcgattga
     6961 agagaatggc ggctgggttg tcggttatga aaactgcacc ggggcgaaag ccaccgagca
     7021 atgcgtggcg gaaacgggtg atgtgtacga cgcgctggcg gataaatatc tggcgattgg
     7081 ctgctcctgt gtttcgccga acgatcagcg cctgcaaatg ctcagccaga tggttgaaga
     7141 atatcaggtc gatggcgtag ttgatgtgat tttgcaggcg tgccatacct acgcggtgga
     7201 atcgctggcg attaaacgtc atgtgcgcca gcagcacaac attccttata tcgctattga
     7261 aacagactac tccacctcgg atgtcgggca gctcagcacc cgtgtcgcgg cctttattga
     7321 gatgctgtaa ggagtggcag tgacatattc gattggcatt gattccggct caaccgccac
     7381 caaagggatc ttactggctg acggcgtgat tacgcgccgt ttcctcgttc caacatcctt
     7441 tcgcccggca acagcaatta ctgaagcctg ggaaactctg cgcgaagggt tagagacaac
     7501 gccgtttctg acgctcaccg gctacggacg gcaactggtg gattttgccg ataaacaagt
     7561 gacggaaatc tcctgtcacg ggctgggcgc acggtttctt gagccagcaa cgcgcgcggt
     7621 aatcgacatc ggtggtcagg acagcaaagt gattcagctt gatgatgacg gtaacctgtg
     7681 cgatttcctg atgaatgaca aatgcgcggc gggcaccggg cgtttcctgg aggtgatctc
     7741 gcgcacgctt ggcaccagcg tcgagcaact cgacagcatt accgaaaatg tcataccgca
     7801 cgccatcacg agtatgtgca cagtgtttgc tgaatcagaa gtgatcagcc tgcgctctgc
     7861 gggcgtagcg ccagaagcga ttctcgccgg agtgattaac gcgatggcgc ggcggagcgc
     7921 caatttcatt gctcgtctct cctgtgaagc gccgattctg tttactggtg gcgttagcca
     7981 ctgtcaaagg tttacccaca tgctggaaag ccatctggga atgccggtac aaactcaccc
     8041 tgatgcgcaa ttcgctggtg caattggcgc ggcggtgatc ggtcagcggc agagaaaacg
     8101 cgcatgaaag agtatctgtt tttatttcac tccacggtcg gcgtcataca aacccgcaag
     8161 gcgttgcagg cagcgggcat gacttttcgc gtcagcgata ttccccgtga tttacgcggc
     8221 ggctgcgggt tatgcatttg gctgacctgt ccgcctggcg aggaaattca atgggtgatc
     8281 cctgggcata cggaatcagt ttattgtcag caggatggtg gctggcgctg cattgcacat
     8341 tacgggattt ctccgcgtta aggattcatt aagatcgcta cgtatacttc tcgatatctt
     8401 attgatttgc cttaaaggga ttaccgtgac aaaaagtatt tcgttaagta aacgcatctt
     8461 cgttattgtg attctttttg tcattgtggc agtgtgtact ttcttcgtgc aatcttgcgc
     8521 ccgtaaaagc aatcatgcag catcatttca gaattaccac gccaccattg acggcaaaga
     8581 aattgcaggc ataacgaata atatctcttc gctgacctgg tcggcacaaa gcaatactct
     8641 ttttagcact atcaacaaac ctgccgccat tgttgaaatg accaccaatg gtgatttgat
     8701 ccgcacaatt cctctggatt ttgttaagga tctcgagacg atcgaataca ttggcgataa
     8761 tcagtttgtc attagcgatg agcgcgatta cgccatttat gtgatttcac tgaccccaaa
     8821 ctcggaagta aaaatcctca aaaaaataaa aatcccgctg caggaatccc caaccaattg
     8881 cggttttgaa ggattggcct attctcgcca ggatcacact ttctggtttt ttaaagagaa
     8941 aaacccgatt gaggtctaca aagttaacgg gctattaagt agcaatgaat tgcatatcag
     9001 caaagacaag gcactacaac ggcaatttac tttagatgat gtgtccggcg cagaatttta
     9061 ccaacaaaaa aatacgcttc tggtgctgtc tcatgaatct cgcgcactcc aggaagtaac
     9121 gctggttggc gaggttatcg gtgaaatgtc actagccaaa ggcagtcgag gtttgtcgca
     9181 taacatcaaa caggccgaag gggttgcgat ggatgcctct ggtaatattt atatcgttag
     9241 cgaaccaaat cgtttttatc gctttacccc tcaatcatcg cattaacagg ctggcatgtt
     9301 tcaccttttt gttattaact tgttaacagc aaaaatgaca aacaaggtta aacatgcctt
     9361 cgccctcgca tcccgtagaa cgcttttctt tcagcaccgc gcttttcggg atgctggttc
     9421 tgaccttagg tatgggttta ggccgcttcc tttatacacc tatgttgccc atcatgatgg
     9481 cggaaggatc gttctcattt agccagctct cgtggattgc cagcggcaac tatgcggggt
     9541 atctggctgg cagtctgcta ttttcttttg gcgcatttca ccagccatcg cgcctgcgcc
     9601 catttctgtt ggcttctgcc ctggcgagcg ggttgttgat cctcgcaatg gcatggttgc
     9661 cgccatttat tctggtgtta ttgattcgcg tcctggcggg tgtcgccagc gccggaatgc
     9721 tgatttttgg ttcgacgctg attatgcagc acacgcgcca tccttttgtg ctggcagctt
     9781 tgttttctgg cgttggcatt ggcatcgcac tgggcaatga atatgttctg gcaggcctgc
     9841 attttgacct ctcctcgcaa acgttatggc aaggtgccgg ggcgctttct ggcatgatgc
     9901 tgattgcact tacgctttta atgccctcga aaaaacacgc catcacacca atgtcattgg
     9961 caaaaacgga gcaacagata atgagctggt ggttactggc tattctgtac ggcctggcgg
    10021 gatttggtta tatcatcgtc gcgacttacc tgccgctcat ggcgaaagat gcaggctcac
    10081 cattgttaac cgcccatctc tggacgttag tcggcttatc aattgtgcct ggttgctttg
    10141 gctggctatg ggcagcaaaa cgttgggggg ctttgccctg cctgacggcg aatttgctgg
    10201 tgcaggctat tagtgtgttg cttactctcg ccagcgactc gcctctcatg cttatcatca
    10261 gcagtcttgg ttttggcggt acctttatgg gcacgacatc tctggtgatg actatcgccc
    10321 gccaactgag cgtaccggga aatctcaatc ttttaggctt tgtgacactc atttacggca
    10381 ttgggcaaat cctcggcccg gcgctaacca gtatgcttgg caatggaaca tcggcgcttg
    10441 ccagcgccac tctctgcggc gcggcggcgt tgtttattgc tgcgcttatc agcacagcgc
    10501 aattatttaa gctgcaagtg gttacttcat gaggagtgtt tcccttacca ctcaagaaac
    10561 tcaaccgcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa agggagccct
    10621 cgtttcgcca tttcctgtgc tttgacggtt aagataaccg gcgaaccgtg tcttgccccg
    10681 actttgcggg ccgttgcttc atcagcagag agatgcacgt aatggcgctc ccctgcaatc
    10741 agcccctgtt tttttatcgc ctcaaggaaa cggcttgccg taccgtggta gagaaattgt
    10801 ggcggtgttt tttcgatgaa ggcaatgacg acctgcgaag tcgaatgccc ctgaacggcg
    10861 cgaatacacg taccatcact ggaataacta aagcgctttt tatcgctggt ggcgaccaca
    10921 gtatccagta atgcgcgggt taaccgcttg cccgcttttt gggcacaaag tatgagctta
    10981 tcgatatccg cccatccttc acggtccagc acgatgccaa tcgcctctgg tttatgtcgg
    11041 aggacaaaac ttaaaaactt gctcgtctct gccagctctt tgtcgttgta ttttgccatc
    11101 gcgttttcgt ccctgaattt tctgttttta tttactgatg tcattgtcac tgggtttatg
    11161 aattttctgc aactcaatcc tgcgcagaac ctttgccaca cagcgtcatt tttccgctat
    11221 gcaaaaaacg catagcaaga aacatacttg cattttcccc atacccacat gactgataag
    11281 gtttttcagc tccaggaatc ttcctggctc aagcaacaca catacacaaa aaaacaatgg
    11341 ggattgttat gacacaacaa ggggatgctg tagctgggga gctggcgaca gaaaaagtgg
    11401 gtgttaaagg gtatctggcc ttttttctca cgattatctt tttttccgga gttttttccg
    11461 ggacagacag ttggtggcgc gtttttgact tttccgttct gaacggatcg tttgggcaac
    11521 tgcctggtgc gaatggcgct accacctcgt ttcgcggcgc aggtggcact ggagctaaag
    11581 atggctttct cttcgcgctg gaactggctc cgtcagtgat tctctctctg ggaattatct
    11641 cgattaccga tggtcttggc ggattgcgcg ccgcgcagca gttaatgacg cccgtgctaa
    11701 aaccgttgtt gggcattcca ggcatctgtt cactggcgct gattgctaac ctgcaaaaca
    11761 ccgatgccgc cgcaggtatg accaaagaac tggcgcagga aggggaaatt accgaacgtg
    11821 ataaagtgat attcgccgcc tatcaaacca gcggtagcgc catcatcacc aactacttct
    11881 cttccggcgt cgcggtgttt gcctttctgg gcacgtccgt aatcgtacct ttagccgtca
    11941 tcctggtgtt taaatttgtc ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac
    12001 gtcgcaaccc aacgcaagga gcgcaagcat gacaactcag gtccgtaaaa acgtcatgga
    12061 tatgtttatt gatggcgcgc gccgtggctt tactatcgct accaccaacc tgctgccaaa
    12121 cgtggtgatg gcctttgtca tcatccaggc gctgaaaatt actggcctgc tcgactgggt
    12181 gggacatatc tgtgaacccg taatggcgct gtggggattg cccggtgaag cggcaaccgt
    12241 ccttttagcc gcattgatga gcatgggcgg cgcagtcggt gttgccgcca gtctggcaac
    12301 cgctggcgca ttaaccggcc acgatgtcac cgttttactg cccgcgatgt atttaatggg
    12361 caacccggtg caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta
    12421 tccgcatatc attacggtct gcgtgattaa cgcattgctg tcgatctggg ttatgcagct
    12481 tattgtttaa caaggagtta ccatgattga ttattccgca gccggtttta ccctgctgca
    12541 gggagcgcat ttgtatgcgc cggaagatcg gggaatttgc gatgtcctca tcgctaacgg
    12601 caaaattatt gccgttgcca gcaacatccc ttctgacatt gtaccggact gcacggttgt
    12661 cgatctcagc gggcagatcc tctgcccagg ttttattgat caacacgtcc atttgattgg
    12721 tggtggcggc gaagcaggtc ccacgacacg cacgccggaa gtggcgttaa gtcgtctgac
    12781 ggaagcgggc atcacgtcag tggttggcct gctgggcacc gactctatct ctcgccaccc
    12841 ggaatccctg ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg cctggatgct
    12901 gaccggcgct tatcatgtcc cttcccgcac tattacgggt tccgtggaaa aagacgtggc
    12961 gattatcgat cgcgtgattg gtgtgaaatg cgccatctct gatcaccgtt ctgccgcacc
    13021 ggacgtttat cacctggcta atatggcggc ggaatcccgc gttggcggtt tgctcggcgg
    13081 taaacctggc gtcaccgtgt tccacatggg tgacagcaaa aaggcgttac agccagtcta
    13141 tgacctgctg gaaaactgcg atgtgccgat cagcaagctg ctgccgaccc acgttaaccg
    13201 caacgttccg ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca ccatcgacat
    13261 caccagcagc attgacgaac cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc
    13321 gggtattccg ctggcacgcg tcaccctcag ctccgacggc aacggtagcc agccgttctt
    13381 tgatgacgaa ggaaatttaa cccatatcgg tgttgcaggt tttgaaacat tgctggaaac
    13441 cgtgcaagtg ttggtcaaag actatagctt cagccttagc gatgccctgc gcccgctcac
    13501 cagtagcgta gccggtttcc ttaacctgac cgggaaaggc gaaattctgc caggcaatga
    13561 cgcagactta ctggtcatga cgcctgaatt gcgcattgag caggtatacg ctcgcggcaa
    13621 actgatggtc aaagacggca aagcctgcgt gaaaggaacg tttgaaacgg cataaacggt
    13681 tgtcagccgc ccgaatggac gtagaatgcc cgcaagggcg gctgacagag tataacgtaa
    13741 tggatgactg tggtgcggtt ttgcataaca ttgaaaccaa atggctttat gattttctga
    13801 ccctggaaaa atgtcgcaat ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccag
    13861 cattcagccg ccgcatccgt gcgctggaac aggcgattgg tgttgaattg tttaaccgcc
    13921 aggtaacgcc gctgcaactc tcagaacaag ggaaaatctt ccattcgcag atccgccatc
    13981 tgttgcaaca gttagagagc aatctggcag agctgcgtgg cggcagcgat tacgcgcaac
    14041 gtaaaatcaa gattgccgct gcacactctc tttccctcgg tctgttaccg tccattatca
    14101 gccagatgcc gccgctcttt acctgggcga ttgaagccat tgatgttgat gaagcggtcg
    14161 ataaactacg tgaagggcaa agtgactgta ttttttcctt tcacgacgaa gatttgctgg
    14221 aagcaccgtt tgaccacatt cgcttatttg aatctcaatt attccccgtc tgcgccagtg
    14281 acgaacacgg agaagcactt tttaacctcg cgcagccaca ctttccgtta ctgaattaca
    14341 gccgcaactc ctacatgggg cgattgatta atcgcactct gacgcgccac agtgagttga
    14401 gtttcagcac cttttttgtc tcttcgatga gcgagctttt aaagcaggtt gccctcgacg
    14461 gatgtggaat tgcctggttg ccggagtatg ccatccaaca agaaattcgc agcgggcagc
    14521 tcgttgtact gaatcgggac gaattagtta tcccgattca ggcctatgca taccggatga
    14581 atacccgcat gaatccagtt gccgaacgct tctggcgtga actgcgcgag ctggagattg
    14641 aacctggctg aaagttttct gcagaaaatc ttccggcgct gggaattatg attgaggttc
    14701 ctgccgcagt catgattgca gacaaattag ccagtgaagt cgatttcttt tccatcggca
    14761 ccaacgatct gacgcaatat attatggcgg cagacagagg aaatagcacc gtcgcgaaac
    14821 tggtcgatta ttgtaatgac gcggtgatta acgctattgc tatggtgtgt caggccggac
    14881 gtaataatga aattccggtc agtatgtgcg gcgaaatggc tggagatatc cagcaaacag
    14941 cacgactgct caccatgggg attgataagc tcagtgccag tccgtcccgg cttccggcct
    15001 tgaaagcagc aatcagaacc tcccactaat tgtgacctaa cgcgatacgt cttgcgcgta
    15061 tcgcgtttcc cgtcatttac tgcggcatcg tgcgatgcca gctccccttc tgccagacca
    15121 ggtcaaacgt tagcggctca tcatcgtggt acagcatcat ctccagacta atggccaact
    15181 cgtggcaata ggcttctttc agattgacct gcaatgccgc aatacgtggc tcatgaaata
    15241 aaagcgcgga acgtaactgg cggcaatacc aactctggag aagcttgtcg ctctgattat
    15301 gcccttgcca ctcaggcatg cctgaaacaa gtaatattgg tagcggtgaa tggggagaac
    15361 taaaaagttg aaagagatcc cggcatacgg aatcacgcag cgataacgta ttgtttcctg
    15421 tactttcagg taataccgtc tgaactgatt ggcgcatcat tttgcgcact cctgacgttt
    15481 agtggcatct gtatcctgcc ctggctgccg gacaccactg ggttgatgct catattccat
    15541 aatcattcct ttatgctcat tgcgtccctg gcactttcat aagggattta taagatccgc
    15601 caaagataaa acactgtaca gaaaagaata tccactgact tttaatttaa aaaggtatca
    15661 tttgacaaaa gtcattaaac ataacggaaa tacgttaaca tattctgatt attccgaata
    15721 atttctcagc gttaaactga tatataatta ttccaaaaca atatatcgta cgtaatgatt
    15781 aatttacgca taatatattc tgcagaacat aattaactat tcagggaaca tacatagtgc
    15841 aaagcggtta aactttgcac cagaaatgag ggatcaggcg tcgactttat ccagcggcca
    15901 tgaatcaaac agatagccat cgaaatagat gtcatcaaca ttagagaact ccagcaagcg
    15961 ctgcttcaca ttctccagat gctgccacat tgccagcttc gccgctcggg catctttttt
    16021 gattaacgcg gcgaggatct gtttgtgatc gcccaaccac tctttgcgat acaggctgtc
    16081 atccaggtgg ctgtgcaact ggatccacat tggattgttt tcccgccact gccaggactg
    16141 acggaataac tccaccagca tgctgttatg cgttgcttcg gcaatagcga gatggaactg
    16201 catgtcaccg ctttcgctgc tgcccggcgc gctggaagcc agttcacgct cttccagttg
    16261 caatgcctgg cgcattttga cgatatcttc gcgggtagcc tgcagggcgg caaactcggc
    16321 gatgttactt tccagtaact gccgcgcctg taacagctca aaaggaccgg catcgttgca
    16381 gacgttggca tccggactgt ctgcgttgtg gctgcctgag ctatcaagaa catagatacc
    16441 tgcaccccga cgcacttcca ccagcccttt gatctccagc atgatcagcg cttcacgtac
    16501 caccgtccgc gtgacatcaa gcatttctgc aatttcacgc tccggtggca gccgttcgcc
    16561 aggattgtac ggcgtcttta tgatcagatc gcggatcatc gccccgactt cctggtaagg
    16621 tctttgcgca gaggtggcag atttcatggt gttgtgtccg ttaggtgcgg ttaatcaaat
    16681 tttttccgga tgcggcgtga acgccttatc cgacctacaa cgggcaattt tacggcaccc
    16741 gttaagctca aactatacca ttagcactgg atgttggcac gtacaggtgg gcaaaaaaac
    16801 gaaaactggt caaccaaata ttagtttgat tgaccagtaa gtctgtaggg taatcagtta
    16861 gttacagcgc agccacacac tcgcgcgcac cgcgctcaca cagctgctga tatgccgccg
    16921 tcaccgcgcc gacaaagtcg gcgttctgcg gcagatcatc ggcaaagata ccgctcaggc
    16981 ccagcagtgc tttcacgcgg tctgcaccct gatactgcgc gttgatcttc tggaactccg
    17041 ccagcatcgg gtcaaccacg tcaatagcat taccctgctc atccacgccc tgggtgtagc
    17101 gcatccagcc agccacgccc agcgccaggt gacgccagct accgccgttt tgcaggtgca
    17161 gacgcaccgg gtccagcaga cgctgcggta acttctggct gccgtccatt gcaatctgcc
    17221 aggtacggtg acgcagagac gggttgctga aacgctcgat cagcagcgtc gcataggcgt
    17281 tcaggtccgt accttccggc atcgacagcg ttggcgcttg ttcctgcatc atcagggcaa
    17341 aggccgcttt gcgataagcc gggttagtca tggtgtcggc aatagtttca tagccgccga
    17401 ggtaaccgag gtacgccagg aaagagtggc taccgttcag catacgcagc ttcatcattt
    17461 cgaacggtac aacatccgca acaaactgcg cgccgacttt atcccagtcc ggacgaccat
    17521 taacgaagtt atcttcaatt acccactgac ggaacggttc gcaggcaatg gcgcacgggt
    17581 cgtaaacacc cagctggtca gcaatttcct gtaaggtttc tggcgtcgcc gccggaacga
    17641 tgcggtcaac catggtgcac ggaaaggtga cgttttcttc aatccatgcc gccagttgcg
    17701 ggtcacgcgc ctgagccagc ccaagaacgg caaccttcgc cacatgaccg ttttcacgca
    17761 cgttgtcgca ggacatcacc gtaaatgctt tcagcccttt ttcacgacgc agacgcaggg
    17821 cttctacgat gtaaccaatc gcggacttcg gcgcagtcgg gttttcgaga tcgtgcttga
    17881 tcagcgggtt attgagatcc agctgaccgc ttgccgcatc agcgcagtag cctttttccg
    17941 tgaccgtcag agagacaatc gccgtctgcg gacgcgccat cgcgttgaga ataccttcgc
    18001 aaccgtcgat ttccggatgc agcgcttctt tcatcgaacc gataattttc agctcggtgc
    18061 tttccgcgcc tttttccgcc acggtgtaca gcagttgctg ttttttcagg ttttcgatca
    18121 gcacgcggtc gttgcctggc atcaggttaa cttcgcagat accccagtcg ctgtcggtac
    18181 tttccagcag atgatgggta tacagcgcct ggtgcgcgcg gtgaaacgcc ccgcaaccga
    18241 gatgcacaat gcgtgattcc agacgagaat gatcccatga cgggcgggca accggcagat
    18301 tgctgtcaac aatagtagtc atcgtaaact ccagaatccg tcttcagaga gtgacggcaa
    18361 ggaagaagga accggcacgg cgcgcagcca tgccggtggg tattaacggc taaagaaagc
    18421 gcgctggatc gccagttcga caccgcgaac ttcggccagg cctttcagac gaccaattgc
    18481 ggagtaacca ggattggttt tcttcttcag gtcgtccagc atctgatgac cgtggtccgg
    18541 acgcatcggg atcaggtctt ctttgccttc cgctttacga cggtgttctt cttcaacaat
    18601 cgctttcacc acttcgtaca tatcaacgtc accgttcagg tgcgccgctt cgtggaaggt
    18661 tttcgggtta tcttcacgca tggtggagcg cagatgggtg aagtaaatac gcggaccgaa
    18721 ctgcttgatc atatcaacca gatcgttgtc agcacgcacg ccgtaggaac cggtgcacat
    18781 ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc cactgcatat cttcaatggt
    18841 ggaaacaatg cgcggcaggc cgaggatcgg gcgcggcgga tcgtccgggt gaaccgccat
    18901 acgcacgccg acttcttcgg caaccggaat aatcgctttc aggaagacag cgaagttttc
    18961 gcgcagtttg gctttgtcga tatctttgta cagctccagg tgcttacgga actggtcgag
    19021 ggtataccct tcttccgcac ctggcagacc ggcaatgatg ttacgggtca gacgcgcttt
    19081 gtcttcgtcg ctcatggtgg cgaagcgttc agttgcctga gcaatttctt cttcggtgta
    19141 atccgcttcc gcgcctggac gtttcaggat atgcatttcg aatgcagcga attcgatctg
    19201 gtcgaaacgc agcgctttgg agccgtctgg cagcacgtat tcgaggtctg tacgagtcca
    19261 gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg ccgcactgcg ccaggttgcg
    19321 cagagtctgc tgatagttag cgatccactg ctcatagttg ccggtgtggg ttttgatatc
    19381 ttcgtgaatt ggcacgcttt ctacgacaga ccataccagg cctgcgtctt caacgatcgc
    19441 cttgcgtttg aggatctctt caacggacca tacttcgccg ttcgggatat ggtgcagcgc
    19501 ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct aaagaaaccg gatcgtttgg
    19561 gccgtaccag cgccaggtct gttccatgat ttttcctctt tcacatagtg ggttcaacag
    19621 cgaagagacg cggaaccccg gagttgttta ggtatccgga tgaacaaccc ggccacagcc
    19681 gtagcgtcgc atcggatgag atggcgtata agttctacct tttggtaaga catgtcaacc
    19741 cgaattctaa aattggttaa ccacatcaca agaatttcac tctcctggtt gttactgcaa
    19801 attgcagcaa agggtaatcg atggttattg atcagaggat gtgacattca tcgcaacaat
    19861 ggttgaccaa tttacataac atgtcgccca aaataacaac ggttcaacca ggtaaggtaa
    19921 aacgttatgc atgtgcttaa cattctctgg gtggtattcg gcattggcct gatgctggta
    19981 ctgaatttga agttcaaaat caattcgatg gtggctttgt tggtggcggc gctgtccgtc
    20041 gggatgctgg ccggtatgga tttgatgtcg ctgctgcaca ccatgaaagc aggtttcggc
    20101 aacacgctgg gggaactggc gatcatcgtg gtgtttggtg cggtcatcgg taaattgatg
    20161 gtcgactccg gcgctgctca ccagatagcg cataccctgc tggcacgtct cggcctgcgc
    20221 tatgttcaga tatcagtgat tatcatcgga cttatttttg gtctggcgat gttctatgaa
    20281 gtggccttta ttatgctggc gccgctggtg atcgtaattg ccgccgaagc caaaataccg
    20341 ttcctgaaac tggctattcc ggcagtagcg gcggccacca ccgcacattc actgttccct
    20401 ccgcaaccgg gtccggtggc gctggtgaat gcttacggcg cagatatggg gatggtttat
    20461 atctatggcg tcctggtgac tattcccagc gtaatctgtg cgggactgat cttgccgaaa
    20521 ttcctcggca accttgagcg cccgacaccg tcatttctga aagcggatca accggttgat
    20581 atgaacaacc tgccctcttt tggtgtctcg attctggtgc cgctgatccc ggcgatcatt
    20641 atgatctcca ccaccatcgc caatatctgg ctggtaaaag atacccctgc ctgggaagtg
    20701 gttaacttta tcggttcctc gccgattgca atgtttattg cgatggtggt tgcattcgta
    20761 ctctttggta ccgcgcgtgg tcatgacatg cagtgggtga tgaacgcttt tgaaagcgcg
    20821 gtgaagagca ttgcaatggt gattctgatc atcggtgcgg gtggcgtgct gaagcagacc
    20881 atcatcgaca ccggcatcgg cgacaccatc ggcatgttga tgtcccacgg taatatctcg
    20941 ccctacatca tggcatggct gatcaccgta ctgattcgtc tggcgacggg acagggtgtg
    21001 gtttcagcga tgaccgccgc cgggattatc agtgctgcaa tccttgatcc agcaaccggt
    21061 cagctggttg gcgtgaatcc ggcgctgctg gtactggcga cggctgcggg ttccaacacc
    21121 ctcacccaca ttaatgatgc ctcattctgg ctgttcaaag gttactttga cctgtcggta
    21181 aaagacacgt tgaaaacctg gggactgctg gagctggtca actccgtggt tgggctgatt
    21241 attgtgctga ttattagcat ggtagcgtaa aagaacacca gatcctgcca ttggcaggct
    21301 ctgactgatg acaaagtcta aaaatgtgcc cggccagtcc cctcgcccct ccggggagag
    21361 ggttagggtg aggggaacag accagcaaag tgcgctttgt ttatgccaga tgcgacgctg
    21421 acgcgtctta tctggcctac aaagggctaa cgtgcaggtt ttagcttcag gtaatattgc
    21481 gtacctgcat tagcaatgcc ctgtgatttc tttattgata aacaaaagtc acgccaataa
    21541 tcgattgcac attccctgca gtcacctgcc ctccggtacg tgcgtaattt gccgttaatc
    21601 ccagacttac cgccgaagtc cctactgctc ctaacgatac cgtgttattc gctggaataa
    21661 tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgcgc gggtgaaaac gacgcggtat
    21721 tggtgaaaat cgagttgccc gcatctgcgg ttgtgccgga taggtaatac cccaggtttt
    21781 ggcttttcgc acaataaacg gtaagaggaa tcggcactga accagggtag tccggcagag
    21841 taacggtgac atcacgagca gaaacatcac agccgccagt gggcaccacc acatcattat
    21901 tggcgtaaat attccacaca aactggaaat catcgctgtt atagttgttg gtctgtcgca
    21961 aaataagcac ggcaattaat gagccagctt taatcgccac tccccccgca ctgctcaccg
    22021 gcgtcaaata aagcgccacc ggccacggct tatccgttct cgaattataa acaacccgcg
    22081 gcgtttcgct ggtagtaggg aaaggatagc tactgccatt atattttacg gtcccggaaa
    22141 aactagataa cacgccgcca taagccgaac ctcgttgcag tgtgacatag tctgtaatgg
    22201 tttctgggta atcgttatgg caaaagattt gcgtcgaaag atctacgacc aggttttgcc
    22261 ccacattcac ggcaggcgca aggtttacat aaacattggc gctgccaccg ccaataggga
    22321 ttgcggtacc attggcggtt ttacaggcga atgaccaggc atttaccgac cagcccatca
    22381 gcagtacagc aaacagggta ataactcgtt tcattacaat catctctttg ggttcagctg
    22441 taggtatagg tgatgctaat cactgcctga atggttccct gagtggctcc accatttacg
    22501 gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg aggaatcatc cacctgaact
    22561 gttttggttg cgccagtatt caatgtgttg ccactgtcat cctgtagctc taactggatg
    22621 ttttgcgcgg tcccctgatt tttataatat ccggtactgt cggctgcccc gctgaagctg
    22681 gcagtgaccc tcgacgttcc caccggacaa ttagtcaact caagcgcaac atcatgccag
    22741 gccgatgccg ccccggcaga catcagactg aaagaataaa gatcgccgag atctaccgtg
    22801 gcattggtgg tggaaaccgt acacggtttg gcgacgaccc taccgttaac cgtgatggtg
    22861 acatcggctg cctgtatcgt cgcacttgcg agcgccaata ttgccgccaa tacataccca
    22921 cgtttgcacc atttcatgag catctccagt tactgatatt caagagtgaa ggtagccgtg
    22981 gcattgatat gccccgcagt gacaggcacc tgtgtcgcca ttagccgggc gtaaaaattc
    23041 aacgtatttg gtttacccgg cgtcagggtc gtccacgaaa tcgcggacga tggggcatta
    23101 aggggtatct cattttgctg ctcattcaga agctgtattc ccagtcccgc agccgctgac
    23161 accgtatttt caagtgcaag caggttggca ttgtggctat ctgcaacgcc agtaaaccca
    23221 acctttacgg cagaaacagc gttaccacag ggtgacagca aaatacgaaa tggaacgaca
    23281 ggagtcgtcg caccaatgtt gttaaattgc ttcgccgcgt tttccatcag atcaacagta
    23341 aaattggttg attcagcggc cacactacag ccattatctc tgacatagcc gcggatagta
    23401 atcgtgctat ccgcagccaa agcgtgactt accgccagcc acaaaaaagc gcaaagaaga
    23461 taaaaaggtt tgtttctcat cacgccccct taacgacatt cagctgatag ctgggttaat
    23521 aactgctgct gactctctgg tggcagttga taattggcga cacagtgagc attttcctct
    23581 tctccccatt tcacctgcac ttttcccgct aaaggcattc cgctgaggta aacctgacca
    23641 ttatccgcaa caatgccgct actctggcta ctctctgatg tcaccatcgc cccaaacggc
    23701 agcggcttat tattgtgggt tagcgtcatg agcagtttta tcccaacgcg cgctttaaac
    23761 tctgctcgca cgatcgcccc acgggtggga acaacgttag cgaccgcatt atctaaatcg
    23821 acgttatcag ccagggtatt ggtatccagc gccactctgt tttcccgata ttcagtggca
    23881 taaggcagca cggcataacc ccgccagtcg gtacgcactc ctgtctggtt ttcgactttt
    23941 gcatcttttg cgccaggcgc tttaacaagt accaccgtat cgtttaacgg ctgccccagc
    24001 gttacgccat tggcatgagc cagtacccca ccgctgactc cgtaatagag ctgcttaata
    24061 tcatcgctat ggctgtaacc gatattggca ttgccgtaac caccgcgata attcagcgtg
    24121 gcgtagcctg tacttccgct attaccatcg cctcccccgg catagccggt ttgcacgcta
    24181 tagctgaggt tgttgtcttc cagcaacgta ccgtatacac cagccagatt ggtcatccga
    24241 ccgttgagat cgtgtgacat gctgtagctg gcactggcat gtcgccactg agatttactg
    24301 tcagaacgca gccagtggct gaaaggaata ttgacgttaa gcgctaacat ctgatcacgt
    24361 cctttttgcc aggcgttttt cgtcaggcta tagctgagcg tccagttgat atcttcgaac
    24421 gcagtattta atccagcctg gaattgctca tcgacattac tcgttcccca ataagtttga
    24481 tggctaccac tcaaatacag tgttgatgtg cgcccgagtt gctgagtaac ggtgagttgt
    24541 aatttcccgc gtttgttata agcgagattg taatagtcgg tgaatttcgg cttaacctga
    24601 ataactccat cctgtgtttc gatgttgtag ccattcattc gactgtatgt tgtatcagcg
    24661 aaattaaaat acccactggt cgaataacgg taacccacta actgaatatt cgtacctgac
    24721 tcattgagcg atttgttata aagaaaacgc accgattgtc cgtcatgctg actgtcatcg
    24781 ggaagtgtgg aattagcctg cgtcatatcc acagacagag cgcccagtgc ccccatattt
    24841 ttcccgatac cgaaattaaa cgcacgataa cgatccgcca gttgcgttcc accatatatt
    24901 gtccagccag ccggaaggcc gtggagtaat gtactctgga aaaagcgggg tttttcctgt
    24961 tgtgcatttc cactacggta ttctcctgcc gtaatggaat aacgtgtatg cccttcacgc
    25021 tgcaaaagcg ggactgacga atagggtacg gtaaaaatct gcgtactgcc gtcagcctct
    25081 ttaatcgtta cctgcaagtc accactatta cctgcggcat agatatcgtt gatggtaaaa
    25141 ggccccggtg gcaccgtact attataaatg tcatacccat tttgtttaat agtgacctgt
    25201 gcagtaccac gagcaatacc gtggatcacc ggggcaaatc ctctttggct atcgggtaac
    25261 atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa taccatcgaa aatatcgccc
    25321 tgagtataac catcacccaa cgtcagccgg gaacgtaacg gaattatgtc tcgctcaagc
    25381 caggtattga tatgctgcca tttatttttg ctacctgacg aactgtcgct actgttataa
    25441 ctccaggtgg tattgtcgcg taaacgccac gcaccaatat ttaacccact ctgtaggttt
    25501 aaatatgcat aatggctgtt acccccaatc cgattctgta cactatttcc gctgaaatta
    25561 taattgagca atccggcatt aataccggga tcccataact caggaggaat ataaccacgc
    25621 gcgcgattac tcataaatgc ctgagggatc gtcaggttga gtcgctgctg acccacatcc
    25681 agttgcgcag tagcgtcatg gatcattgag gttaatggca cgcaggcatc atccgccagc
    25741 agattcatac cggagacaga agccgtattc agccccatac tggcaagttg cgcgcgtgtc
    25801 aggcagggaa caatcccttg ttcactgtcg cccgtgttaa atgtgacatc acgcgttgcc
    25861 atataaccat tattcaaata gatatcgaca cgatacgtcc ctggcggtaa ttcttgccca
    25921 ttctcaaaac gcgataaatc agccacagcc tggggatcat ccgctaaaaa acgcgggtta
    25981 aaatagagtt cggcagatga caaaggtgcc tgtgcggcaa aagcacaggc aacaaagagc
    26041 cggacaaaaa aaccagccaa acgatgctta cgaatatgca agcattgtgt gtttcgctgg
    26101 taaagtctta aattcagata tgacattatt cctccggttt aaaatcaccc ggcagtcaat
    26161 tctttttatt caggcgaaaa attccccctg cgttattcca ttacgcccgt cattttgggg
    26221 gtaagtgcgc cataatcatt tattgttcgg taagtaatat tgcttcctgc atcagaaggc
    26281 aatttaaccg tgctttcgcc cattggaggc accaatgcat tttcaagaac ccgggttccg
    26341 gcattcaact ctgttaccgt caggtaatag ggtgtcgggt taatcagcgt cagagaattc
    26401 gcgctacgac gaaatcttaa tttttctgcg gcctgatcgg gtggcaacgc taatttagcc
    26461 ggacgatagt acagtttaat gcggctgata attgcgagct gtagcgtatt ctcagtcaat
    26521 tttgatttat ccattgacgg aatcgcttta acattcatcc agaataaact ttcccgatcc
    26581 tgtggcaatt ggttatttgt tgcatcaaga atacgcaagg tattctcttt ttttcccttc
    26641 atcgcaaaca gaggtggcgt cacgataaaa cggccatcct ttacaccatc ggcattttct
    26701 acccatgatt gaattaaata ggtgctattt tcatcattat ttgtcacggc aagttgcact
    26761 tgtttttgcc ctgccggata aattacgcga gtcgcaccta aggccactcc cgcttcagcg
    26821 cgtccggcaa ccatcattgc catgaacatc aggatacctg ccagcaagca gaatgttatt
    26881 tcctgcgatt tccttacatt gacgttttta ttactcactg tcctgttcct gttatcacat
    26941 tatctgctga acaattactg ataggttaaa gagaaccagg cctgggcatt ggcgatgccg
    27001 ccagtaaccc gacgcccggt agcacgatat ttggcgatga aatgtagcga agtagagcct
    27061 gagtaaagcc gtttccagtt tgctggagga cggttaatcg gtacgaggtt tccttcatca
    27121 tcaaacaacg ctacaccaat attggtggct atccctggcc cctctcccac ggaaagcaca
    27181 tccggatttt taccatccgc gacaccgtga aacgccacac ccacacgttc actcaccacc
    27241 gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg cgctatcttc ccctaccgca
    27301 tgaaaccggt tactgctgat ttgccccata ttgaccgtca tttgtttatc accggcttca
    27361 atccggcaag cttccgcaat aatgacgccc tgaaattgca tatttccgcc gggcagcgtg
    27421 gtattccatt tatttccggc cagagcaaac attggcagca acgacgctaa cagaaataat
    27481 ctcttccttt tcatcgttat ttttatcgca caagggtggg cgtccctgcc cgtaatgacg
    27541 tccctgaacc tgggtaggtt attgatactg aaccttgaag gtcgcatccg cattagctgc
    27601 gccaggggtt gcggcaccaa tggcataata acgcgcctgg aacggaatgg tgttggttcc
    27661 gttattcagg gtggtttgtg cactaaatga cgcaccatcc aaagccaacg cagtccctgt
    27721 tttatccagg atctgcacac caacgtttgt tgcgctaccc gcagcagaac tttgcagagc
    27781 cagtacgttg gtatgattcg tgtcaattgt tgtacctaag aaggcaacag cagctttgct
    27841 tgcaacatcg gtatcgcaat cattcagttg aatgttaaaa ccgacagcag agctggtcgc
    27901 tccatcctgg tccagcgatg cggtacgaac ctgtcctaac tgaacggttt gatcaacaga
    27961 gcctgcatca actgcgcaag cggcgttaac aacttcccct ttaaagtgaa cggtcccacc
    28021 atttaccgtc gtagtatcgg ccagagccgc tgcggaactg agggacagag ccgacagaac
    28081 aacgattgcc agagttttaa ttttcatgct gctttccttt aaaaaaacta tttctaaatc
    28141 gacatgggca gtcgttctgt acactttgtt ttgtcaacga gtttgactgc caacactgca
    28201 cagttttccc ccaaaagatg aaacatttgg ggccattttg actcatagag gaaagcatcg
    28261 cgcacaaact ttttcagttt atttgttggc ttaatgttct atagttatat ttatttgcag
    28321 ttttttatat tgcatgaggt ggtttttgga gagaagaatg aggaagttgc gtcgagctac
    28381 agaaacgtta gctttacata tagcggaggt gatgtgaaat ta
//
LOCUS       DADTFG010000057        28399 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.057, whole genome shotgun sequence.
ACCESSION   DADTFG010000057 DADTFG010000000
VERSION     DADTFG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.057"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(33..635)
                     /gene="fimB"
                     /locus_tag="J1138_002203"
     CDS             complement(33..635)
                     /gene="fimB"
                     /locus_tag="J1138_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HBA8454000.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2099..2815
                     /gene="nanC"
                     /locus_tag="J1138_002204"
     CDS             2099..2815
                     /gene="nanC"
                     /locus_tag="J1138_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBA8454001.1"
                     /translation="MKKAKILSGALLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2835..3941
                     /locus_tag="J1138_002205"
     CDS             2835..3941
                     /locus_tag="J1138_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBA8454002.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAINNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPIGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4006..4986
                     /locus_tag="J1138_002206"
     CDS             4006..4986
                     /locus_tag="J1138_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBA8454003.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRSDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(5569..6705)
                     /locus_tag="J1138_002207"
     CDS             complement(5569..6705)
                     /locus_tag="J1138_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454004.1"
                     /translation="MYLYEHRRCFNNIIGYCNSLCYHGKLQPKRGMEKGTIFPAMGYL
                     QIDGRGMKPNGGSRYNPLEAETIAAWLVAHKDDIERHYGEPLYKVVGVVTPFSAQVNA
                     IKTSLRKLEINGKDEQGSLTVGTVHSLQGAERAIVLFSPVYSKHEDGRFLDSNNSILN
                     VAVSRAKDSFLVFGDMDLIEMQPAFSPRGLLAKYLFSSDNNALQFEFQKRQDLISAHT
                     QISTLHGVEQHDGFLNKTLAGAQKKITIISPWLSWQKVEQTGFLASMALARSRGIDIT
                     VVTDKNCNIAHVDDDKRQEKQHLLNDAVEKLNKMGIATKLVNRVHSKIVIEDEELLCV
                     GSFNWFSAAREDKYQRYDTSLVYRGEGVKNEIKAIYGSLDQRQL"
     gene            6873..7172
                     /locus_tag="J1138_002208"
     CDS             6873..7172
                     /locus_tag="J1138_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA8454005.1"
                     /translation="MLGVLTLARDIQLAIVNKMTQVLIGDIYLRQQYDVFWLGYTISP
                     AYTRQGYAIEAITATIDWIKENGFSLIKAGVNPENTLSKKLLIRIGFNFSSIEDE"
     gene            7549..7806
                     /locus_tag="J1138_002209"
     CDS             7549..7806
                     /locus_tag="J1138_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454006.1"
                     /translation="MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYQINTTELNKVRAQLDNR"
     gene            7818..8369
                     /locus_tag="J1138_002210"
     CDS             7818..8369
                     /locus_tag="J1138_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA8454007.1"
                     /translation="MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDES
                     ARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
                     QPMTLTGHIRCAQALMKPEHWRE"
     gene            8421..9167
                     /locus_tag="J1138_002211"
     CDS             8421..9167
                     /locus_tag="J1138_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA8454008.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPGTRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQAKRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGAEELLAQSLKPGGIMLIGEPYWRQLPATEEIAQACGVSS
                     TSDFLTLPGLVGAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNIAPKRYVTYARECFGWGVFALIAR"
     gene            9294..9554
                     /locus_tag="J1138_002212"
     CDS             9294..9554
                     /locus_tag="J1138_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454009.1"
                     /translation="MSQYEQFCQACGMPMSAPDAHSASDQYCAYCSDSNGNLKPWEEA
                     VSGLASFLDSWQKVGPEEAHKRAKRYLTAMPAWAHKAESDLS"
     gene            9953..11074
                     /locus_tag="J1138_002213"
     CDS             9953..11074
                     /locus_tag="J1138_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBA8454010.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTTELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            11071..11349
                     /locus_tag="J1138_002214"
     CDS             11071..11349
                     /locus_tag="J1138_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA8454011.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            11361..12674
                     /locus_tag="J1138_002215"
     CDS             11361..12674
                     /locus_tag="J1138_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA8454012.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLMPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            12687..13493
                     /gene="sgcQ"
                     /locus_tag="J1138_002216"
     CDS             12687..13493
                     /gene="sgcQ"
                     /locus_tag="J1138_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HBA8454013.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            13624..14055
                     /locus_tag="J1138_002217"
     CDS             13624..14055
                     /locus_tag="J1138_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA8454014.1"
                     /translation="MINDIKWVQAQREATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLAAVGNASTDDALFALVSG"
     gene            14067..14699
                     /locus_tag="J1138_002218"
     CDS             14067..14699
                     /locus_tag="J1138_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HBA8454015.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMVARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            14716..15498
                     /locus_tag="J1138_002219"
     CDS             14716..15498
                     /locus_tag="J1138_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8454016.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IANQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            15801..16589
                     /locus_tag="J1138_002220"
     CDS             15801..16589
                     /locus_tag="J1138_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBA8454017.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVECGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            16594..17499
                     /locus_tag="J1138_002221"
     CDS             16594..17499
                     /locus_tag="J1138_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBA8454018.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            17510..19477
                     /locus_tag="J1138_002222"
     CDS             17510..19477
                     /locus_tag="J1138_002222"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HBA8454019.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPNGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQMKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDV
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTQLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            19584..20933
                     /locus_tag="J1138_002223"
     CDS             19584..20933
                     /locus_tag="J1138_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HBA8454020.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            21280..22266
                     /locus_tag="J1138_002224"
     CDS             21280..22266
                     /locus_tag="J1138_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8454021.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(22307..23311)
                     /locus_tag="J1138_002225"
     CDS             complement(22307..23311)
                     /locus_tag="J1138_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HBA8454022.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVVCVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNRLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(23415..24056)
                     /locus_tag="J1138_002226"
     CDS             complement(23415..24056)
                     /locus_tag="J1138_002226"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBA8454023.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGKPEAGKIPSSEWLIHLECYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(24087..25202)
                     /gene="mtnA"
                     /locus_tag="J1138_002227"
     CDS             complement(24087..25202)
                     /gene="mtnA"
                     /locus_tag="J1138_002227"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HBA8454024.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQDMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGAEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPERTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(25212..26471)
                     /locus_tag="J1138_002228"
     CDS             complement(25212..26471)
                     /locus_tag="J1138_002228"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HBA8454025.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWSTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
     gene            complement(27211..27309)
                     /locus_tag="J1138_002229"
                     /pseudo
     CDS             complement(27211..27309)
                     /locus_tag="J1138_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27399..27767)
                     /locus_tag="J1138_002230"
     CDS             complement(27399..27767)
                     /locus_tag="J1138_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBA8454026.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(27930..28151)
                     /locus_tag="J1138_002231"
     CDS             complement(27930..28151)
                     /locus_tag="J1138_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBA8454027.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(28214..>28399)
                     /locus_tag="J1138_002232"
     CDS             complement(28214..>28399)
                     /locus_tag="J1138_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBA8454028.1"
                     /translation="HNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDH
                     LIVGGSQVFSFAEYGLL"
BASE COUNT         7388 a         6886 c         6882 g         7243 t
ORIGIN      
        1 ttttaacacc atccctggta tctcaactat ctctataaaa cagcgtgacg ctgtcgtcct
       61 ctggctctat cccagatgcc gtaaaaacgc cctgcattgc tggcggtata ccagacagta
      121 tgacgaatat tgcgatgccc aagataatcc tggataagtc gcgtatctat tcccatattc
      181 gccaaagcaa aaccacacga atggcgtaac atgtgcggat gaatctccag tgacaacccg
      241 gcattaccac cggaagtcga gataatatgg taaaactgtt gccgagaaag tggattcccc
      301 ttacgtgata aaaataccca ctcgctctca gcatgcggat aagaagtacg gatactcaac
      361 cagtttttta aagcctgaat ttctttgttc aatagcgggt gcgttgttga aaagcctttt
      421 tttaatcgat ggatatatat acactttgcc ttaagatcaa tatccgaaat cctcaatcgg
      481 caaatttcac tcgcccggaa accatgaata aaacaaagta atgtcagaca ataattacgt
      541 gcagcatgag gcccggtatt tgctgcttta aggagtgatt cgatttcact atgggtcagg
      601 aagttccttt ttttgttatc agccttattc ttcatcgttt tcccttataa ttacagacgc
      661 gcactagctg tgctgggtta gcaataatcc aacattttaa tcctgattat gtttataaaa
      721 gcgaacgttt gcttaataac taacgtaagt ggaccagttc ttctgagtga acttaaatgg
      781 agtagcgata gttacttata ataactttgc catacaaacg ccatgctctt gcatgctatg
      841 tacctttata tatttatcaa tctgatacga atcgaataat aaccctcatc attaatgcaa
      901 ctaatcttat ctatatatca tgtgatatgt tctgtaacaa gtaaaatcaa cataaaaaat
      961 tcagcaaaat ttaatatttg ttcaactagc cgtctcacat atgacattat tagtcaactg
     1021 catttgccac aaaatggcta tttcatggaa cgcagtaatt tcttagtggc tattatcatg
     1081 ctaatgctta ggtccctagc gattattcct gcctggttcg tcagcttacg ccatgagatt
     1141 gttacattcc tttttcagac tgtgtatttc ctggagatat gggggaacgc aggcgacgcc
     1201 ttttcatcta cattcgttgg taaatatggc tttggtcact gcgacttaaa ccttcctcaa
     1261 tcacagctat tcgccgggcg atacggcaaa caaactatct gaaacatcat cctgaaaaca
     1321 ccaacatcta caagcctttc cagatcgact tcaggggtga ccacttacaa gccaaaacca
     1381 ggaaacatat ttcatgatga gaattatgat catcctgatg gcgggtaaac acttgttttc
     1441 accacgctca ctgagttagc ttgcaaagct tcccttgttt agaccctaaa taaatttttt
     1501 ataaaacaaa cggttaaaag aaatcattta gtttttgaag accacctcaa gtgttcgtct
     1561 ggcttcacat ggcatcttcc tcttagaaaa agatcgacat attttgtgac acgaattgca
     1621 aatctcgttt tgttgtatgg attgcgtgat ttttgatctg gtataacagg tataaaggtg
     1681 caccgcgata gtcaatgaga cagggcatct cgcaatctat ggcaaacatc acttcagttc
     1741 tttctcatcg ggtgatgaaa acgcacttca gtctgaaagg aatatgaaaa tgagatcaac
     1801 agacactcta ttttatgact ctgggtaaaa tggattgagt aagtgatata gcttacgaac
     1861 attcaaatca attaaacatc agaagagatt ttatactcag gtatttaatc tggttctctg
     1921 tttatttaaa taatgtggaa agagaatttt cacaggagac cttatacaaa aaatataaaa
     1981 tacagctact cgttgccaaa gacactataa gccaggcaaa aaatattaca caacataaat
     2041 gctaattgtt tatgcgggct ttgtattgct ttctgtatcc tacaaatgag tgaaatatat
     2101 gaaaaaggct aaaatacttt ctggcgcatt attactgtgc ttttcgtcac cactgatttc
     2161 tcaggctgca acactggacg tacgtggtgg atatcgtagt ggaagccacg cctatgagac
     2221 tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg gcaagcatgg aaagtaatac
     2281 ctggaatacc attcatgata ataaaaagga aaatgccgca ctcaatgatg ttcaggttga
     2341 agttaattac gcgattaaac ttgatgatca atggacggtg cgcccgggaa tgttaacgca
     2401 ttttagcagc aacggcacac gctacggacc ctacgtaaaa ctgtcctggg acgcgacaaa
     2461 agatcttaat tttggcattc gctatcgtta cgactggaaa gcttaccgac aacaagactt
     2521 atccggtgat atgtctcgtg ataacgttca tcgttgggat ggatatgtca cttaccatat
     2581 taatagtgat ttcaccttcg catggcaaac gacgctatac agcaaacaga acgattatcg
     2641 ctatgcaaac cataagaaat gggcgacgga aaatgcattt gttctacaat accatatgac
     2701 gcccgatatt acgccataca tagaatatga ctacctggac cgtcagggtg tttacaacgg
     2761 cagagataat ttatcggaaa acagttatcg cattggtgtg tcatttaaac tgtagtagac
     2821 aggagacagt cacaatgaat aaaacaataa cggcgcttgc tatcatgatg gcttcatttg
     2881 ccgcaaacgc gtctgtatta ccggaaactc ctgtgccatt taaaagtggt accggagcaa
     2941 ttaataacga cactgtctac attggtttag gtagcgcagg tacggcatgg tacaagctgg
     3001 agacacaggc caaagataaa aaatggacag cgttagctgc attccctggc ggaccaagag
     3061 atcaagcaac ctctgcattt attgatggca atctgtatgt gtttggcggc attggcaaaa
     3121 acagcgaggg cttgactcag gtatttaatg acgtacacaa atacaacccc aaaaccaata
     3181 gctgggttaa attgatgtcg cacgcaccga taggcatggc gggccatgtg acttttgtac
     3241 acaacggcaa ggcttatgtt actggcggtg ttaaccagaa tatcttcaat ggctattttg
     3301 aagatctcaa cgaggctgga aaagattcaa ccgctgtaga taaaatcaat gcccactatt
     3361 ttgacaaaaa agcagaagat tatttcttca ataagtttct gttgtctttt gatccctcaa
     3421 cacagcaatg gagttacgct ggcgaatctc cctggtacgg aacggctggt gcggcggttg
     3481 tgaataaagg tgataaaacc tggcttatta atggcgaagc caaaccagga ttgcgaacgg
     3541 atgccgtatt tgaacttgat ttcaccggta ataatttaaa atggaatagg cttgctcccg
     3601 tctcatcacc agatggcgtc gctggcggtt ttgcggggat aagcaatgat tctcttatat
     3661 ttgccggagg agccggattc aaaggttcac gagaaaatta ccagaacggt aagaactatg
     3721 cgcatgaagg cctgaaaaaa tcatatagca ctgatattca tctttggcat aacgggaaat
     3781 gggataaatc gggtgaatta tcgcaaggtc gggcctacgg agtatcattg ccctggaata
     3841 atagtctatt gattattggc ggtgaaactg caggcggcaa agcggtgacg gattcagttt
     3901 tgatctctgt gaaggataat aaagtcacag tacaaaacta acgcttcagg gcctcagtaa
     3961 aggggccctg ctatcaattt atcatttcaa ttacgcgaaa attatatgga cgcaataata
     4021 tcgcccgatt attactatgt tcttaccgtt gctggtcagt ctaatgctat ggcgtatggc
     4081 gaaggactgc cattaccgga cagggaagat gcgcctcatc ccagaattaa acaattagcg
     4141 agatttgcgc atacgcatcc cggaggcccg tcatgtcact ttaacgacat tattccactg
     4201 actcactgcc cacacgatgt tcaggatatg cagggttatc accatcctct ggcaacgaat
     4261 catcaaacac agtacggcac cgttggccag gcactgcata ttgcacggaa attactaccc
     4321 tttattcctg ataatgcagg ggttctcatc gttccatgtt gccgtggcgg atcggctttt
     4381 accgcgggca gcgaagggac atattcagaa cggcacggag ccagccatga tgcttgtcgt
     4441 tggggaacgg atactccgct ataccaggat ttagtcagca gaacgcgagc cgcactggta
     4501 aaaaacccgc agaacaaatt cctcggcgta tgctggatgc aaggcgaatt tgacttaatg
     4561 accagtgact acgcgtcaca ccctcaacac tttaatcata tggttgaagc ctttcgtagt
     4621 gatctaaaac aataccattc tcagcttaat aatattactg acgcgccgtg gttttgcggc
     4681 gataccacct ggtactggaa agaaaatttc cctcatgcgt atgaagttat ttatggcaat
     4741 tatcaaaata atgttttagc caatattatt ttcgtcgact tccagcaaca aggtgaaaga
     4801 ggactgacga acgcgcctga tgaagatccg gacgatttaa gcacgggata ttacggttca
     4861 gcgtaccggt caccggagaa ctggacgacg gcactgcgaa gcagtcattt cagcgcggca
     4921 gcccgtcggg ggattatttc tgacagattt gtagaagcaa ttttgcagtt ttggcgcgaa
     4981 aggtgagcgg ggagtttatt catgggggat gtccttgtcc gttcccagag cagacaacaa
     5041 aaaacaaatg agatcaaggt aatatctctt cacttgcggg ctcgaacagc aggcaattgc
     5101 caatataata atctggcaca agggtgaaaa acgcgcaccg cataaaccgt tgttattgct
     5161 tacagattag ccggatgcct taatagacat ccgcgccttt tcggttatgg ctcggaaatc
     5221 tacgaacatc tacacagttt actggaacgt tttgggccac agcgttcgca gcaccgtcct
     5281 gatatgccgt tctggcgatt ataagtcgac ggattctggc aaatagacga tgatgatatg
     5341 cttaatgatg ttcaaagaaa ttaggccatt attatattat tgcttgcatt ttctttcatt
     5401 cctaaaagca cccgccatta atcccaggcg cattatgcaa aggaaaatga ttaggtaaac
     5461 aataagattt ataatcagga aaggcgactc gcaataaaac ggaaagtcgc cttaacattg
     5521 gtaaaatatg ccctttacta ctcctgtatc atgaacggct catcacattt aaagctgacg
     5581 ttgatccaga ctaccatata ttgcctttat ttcatttttt acgccttcgc cacggtacac
     5641 caaagacgta tcgtatcgtt gatacttatc ttcccgtgca gcactgaacc agttaaaaga
     5701 tccgacacac agcagttctt catcctcaat cacaattttg ctgtggacac gattaaccaa
     5761 tttcgtagcg atacccatct tattgagctt ttcaacagca tcattcagca gatgttgttt
     5821 ctcctgacgc ttatcatcat cgacatgcgc tatattgcag tttttatccg tcaccacggt
     5881 aatatcaatg ccccgggaac gggccaaagc cattgatgcc agaaatcccg tttgctccac
     5941 tttttgccaa gataaccaag gggaaataat cgttattttc ttttgagctc cggcaagagt
     6001 cttgttcaaa aatccatcat gctgctccac accatgcagc gttgaaattt gtgtatgtgc
     6061 ggaaattaaa tcctgtcgtt tctgaaactc aaattgcaac gcattattat ctgaagaaaa
     6121 gagatatttt gccaataacc ctcgcggcga aaatgcgggc tgcatctcga taaggtccat
     6181 atcgccaaat accagaaaac tatctttagc gcgtgagaca gcaacattga ggatactgtt
     6241 gttgctatca agaaacctgc catcttcatg ttttgagtag acaggagaaa agagaacaat
     6301 cgccctttcc gccccctgca aggagtgcac agtccccact gtcagtgaac cctgctcgtc
     6361 tttgccatta atttccagct tacgcaatga cgttttgata gcattaacct gcgccgaaaa
     6421 tggcgtcaca acaccaacaa ctttgtacag tggttccccg taatggcgct ctatatcatc
     6481 cttatgtgcc accagccatg ctgctattgt ttcagcttca agagggttat aacggctccc
     6541 tccatttggc ttcatgcctc taccatcaat ttgtaaatat cccattgcgg ggaaaatcgt
     6601 ccctttttcc atccctcttt taggctgcaa cttaccgtga tagcaaagtg agttacagta
     6661 gccgatgatg ttattgaaac agcgacgatg ctcatacaga tacattccgc gctcgaactt
     6721 gggatcatac tggtagtgag agttgaactg ggctacctac attacgctgc cagatgctgt
     6781 actttttcca gacccattta ttatcgaata cggtgaccgc ctgagcggcc acctttaatc
     6841 gtgggcagtt acacagaatt atttacaggg ccttgctggg agttcttaca cttgctcgcg
     6901 atattcaatt ggccattgtc aacaagatga cgcaggtttt gattggtgat atctatttac
     6961 gtcagcaata cgatgtgttt tggctcggct ataccatcag tcccgcttat actcgccagg
     7021 gatatgcaat tgaagcaatt accgccacga ttgactggat aaaagagaac ggtttttcac
     7081 tcatcaaagc cggtgtgaat ccggaaaata ccctttcaaa aaaattactt atcaggattg
     7141 gcttcaattt ttcgagcatc gaagatgagt gagcatattt atgttctgga ttaaacaaaa
     7201 ggagaataat ctcccgaagg gaaaagccga tgaggccatt tcaggcgtac ttcaacatgc
     7261 attcgtcagt gtcatctgcc ttttccagta aatgggttgg cattcaattt ccaaagtaca
     7321 tcacactttt tctactgaaa catatccctc ctataactac gtgttaatat tcgctccgta
     7381 aatacctttc aactcaaccc gaggctttga tgctggagaa tgtaatcatc cgataatcag
     7441 ttccccgacc ttttcaggcc ggactgatta tcaatgcgcc gaaatcgaat gcggacaccg
     7501 cggtgtgttt gcacgttttg cacatgatga ttaaataggt tcttcagtat gaatttatcc
     7561 cgtcaggaac aacatacctt acacgttctc gctaaaggta gacgtattgc gcacgtccgc
     7621 gattcttcag gccgcgtcac ttccgttgaa tgctacagcc gcgaagggct gttgctgacc
     7681 gactgcacgc tcgccgtctt caaaaaactc aaaaccaaaa aacttatcaa gtccgtcaat
     7741 ggccagccct atcaaatcaa caccaccgag ctgaataaag ttcgcgctca gctcgataat
     7801 cgctaaggag agtttctatg acagtacacc acttcacttt tcatatcaca gataaaagtg
     7861 atgccagtga tatccgggaa gtcgaaaccc gcgcatttgg cttcagcaaa gaagccgatt
     7921 tagtggcctc tttacttgag gatgaaagcg cgcgccccgc gttatcgctg ctagcccggt
     7981 atgaaggcaa agccgtcggg catattttat ttacccgggc gaccttcaaa ggagaaatgg
     8041 attcaccgct gatgcacatc ctcgctcccc tggcggtcat accggaatac caagggatgg
     8101 gcgtgggagg acggttgata cgcactggca tcgagcacct gaggttgatg ggctgccaga
     8161 cggtttttgt tctcggccac gccacctatt acccccgaca tgggttcgaa ccctgtgccg
     8221 gagacaaagg ctatccggca ccttatccca tcccggagga gcacaaagcc tgctggatga
     8281 tgcaatcact caccgctcag cccatgaccc tgaccggcca catccggtgc gcgcaggccc
     8341 tgatgaaacc ggagcactgg cgtgaataac aatcttgttg tgtccttttt aaggcttaac
     8401 ccaataacag gaattaaata atggatatcc cacgtatttt taccatcagc gaaagtgaac
     8461 accgcatcca taacccgttc accgaagaga agtacgccac gctgggtcgc gtgctgcgca
     8521 tgaagccggg cacccgcatt cttgacctcg gcagcggctc gggcgagatg ctctgcacct
     8581 gggccaggga tcatggcatt acggggactg gcatcgacat gagttcgctc ttcaccgcgc
     8641 aggcaaagcg tcgcgcggaa gaactcggcg tcagcgaacg cgtacatttt attcataacg
     8701 atgcggccgg ttacgtcgca aatgaaaaat gtgacgtggc ggcctgcgtc ggcgcgacat
     8761 ggattgccgg tgggtttgcc ggggcggagg agttgctagc gcaaagcctg aaaccgggcg
     8821 ggatcatgct tatcggcgaa ccctactggc gtcagctacc agcaacagaa gaaatagccc
     8881 aagcctgcgg cgtcagctcg acaagcgatt tcctgacgct gccaggactt gtcggagcgt
     8941 tcgacgatct cggctatgac gtggtggaaa tggtactggc agaccaggaa ggctgggacc
     9001 ggtatgaagc cgcaaaatgg ctgaccatgc gccgctggct ggaagcgaat cctgacgatg
     9061 acttcgccgc cgaagtcagg gccgaactaa acatcgcgcc gaaacgctac gtgacctacg
     9121 cgcgggaatg ctttggctgg ggcgtgttcg cgctcatcgc caggtaacaa caggttcccg
     9181 gtatttctgg ctggaaatat cgaggcttta cctttgcttt gatctgatgt atcacctgca
     9241 caatacatat tcagtatgtt tattaatcaa gatgtcacca taaggagcat catatgtcac
     9301 aatatgaaca gttctgtcag gcttgcggaa tgccgatgtc ggcaccagat gcacacagtg
     9361 ccagtgatca atattgtgcg tactgcagcg acagcaacgg caatctcaaa ccttgggaag
     9421 aagcggtatc aggtttagcc agcttccttg attcctggca aaaagtcggc ccggaggaag
     9481 cacataagcg tgcaaaacgt tatctgacgg caatgccagc ctgggctcat aaagcggaga
     9541 gtgatttgag ctgattatct atactccctc tgaatctatt caggagatcc gatgttttct
     9601 tacattatgc tgggaaccaa cgacctgcca cgcgccatta tattctaaga tccgctgatg
     9661 gagatgctgg ggtatccacg tgcaggacgt aatgaagagg gagcgtcctg caacaaagtg
     9721 gtgcgataac agttaccgcg ttgtattcat cctcattatt tcaaccataa tcactggtct
     9781 ctccagcccg taactttcac aaacgttgag cgcttttatt ccactcggct cacataattt
     9841 tttatttgtt cagctttgcg cgtcttcacg tgaacgttct cacaattatt cgaccagtct
     9901 gcgcctataa aacgctcaac gagtgattgt cttacggtct acggagatag ctatgtcatt
     9961 ttctgtgcag gaaaccctgt tctcgctttt gcagcacaat gcgatttcag gacacgaaaa
    10021 cgctgtcgct gacgtcatgc tgtgcgaatt caggcgtcag gcaaaagagg tctggcgaga
    10081 caggcttggg aatgtcgtcg cgcgctacgg tagtgataaa cccgatgcgc tgcgactgat
    10141 gatttttgcg catatggatg aagtcggttt tatggtgcgc aaaatagagc cgtctggatt
    10201 tttacgcttt gaacgcgtag gcggtcctgc gcaggtcact atggcaggtt ccatcgtcac
    10261 cctcaccggg gacaaaggac cagtcatggg gtgtatcggc attaagtcct accactttgc
    10321 caaaggcgac gagcgcacgc agtcaccttc tgtcgacaaa ctgtggattg atattggtgc
    10381 caaagacaaa gacgacgcta tacggatggg cattcaggtc ggtacgcctg tcactctgta
    10441 taacccgccg caactcctgg caaacgatct ggtgtgcagt aaagcactag acgatcgtct
    10501 gggctgtact gccctgctcg gtgtagcgga tgctatcagt actacggagc ttgatatcgc
    10561 cgtttatctg gtggcttcgg tacaggaaga atttaatatc cgcggcattg ttcccgtatt
    10621 acgccgtgta aaacctgacc tggcgattgg tattgatatc actccatcgt gtgacacccc
    10681 tgatttacac gattattccg aggtcaggat taatcagggc gttgggatca cctgcctgaa
    10741 ctaccatggt cggggaacgc tggccggatt aatcacgcct cctcgcctga tacggatgtt
    10801 ggaacagacg gctcttgaac acaacattcc ggtgcagcga gaagtggctc ccggcgtgat
    10861 aacagaaacc ggttatatcc aggttgagca ggatggtatt ccctgcgcca gtctctctat
    10921 tccttgtcgc tatacccatt ctccggcgga ggttgccagc ctgcgtgatt tgactgattg
    10981 catccgccta ttgaccgctc tggcaggtat gtcagcagca catttccccg ttgagcctga
    11041 ttcaggcact acacaagagg cacatccatt atgaaaaaga tccttgtggc atgcggtacc
    11101 ggcatgtcga cttcaaccat gattgcacac aagctgcaag agttcctgac tgaacagggc
    11161 atttcagcca ctaccgccca atgctgtctg aatgagatcc ctcttaactg taacggtatg
    11221 gaccttattg taacctccat gcgaaccaac agtgattacg gcatccctac gcttaatggc
    11281 gccgccctgc tcacagggat caacgacgac gcgttaaaac aacaaatcaa ggctctgtta
    11341 actcaataaa aggaggcatt atgtttgatt acatcctgtc tctcggcggt accgttttcg
    11401 tccctatcat catgatcgtg attggcctta tcttccgcat cccctggctg caggcgatta
    11461 aggcaggggt aacggttggg attggttttg tcggtatggg actggtgatc gtcatggcga
    11521 tcgacagcct cagtccccct atcaaagtga tgattgagcg tttcggcctg gccctgcatg
    11581 tctttgacgt tggcgcaggc cctgcctccg gcgttggtta tgcgacagcc attggcgcca
    11641 tgattatccc agttattttt cttctcaatg tcgccatgct ggtcacccgt ctgaccaaaa
    11701 cgatgaacgt cgatatttac aattactggc actacgccat tacgggtacg gtggtccagc
    11761 ttatgaccgg tagcctgatt tatggtgtgc tgggggcaat ctgccatgcg gcgctatcgt
    11821 taaaaatggc cgactggacg gcaaaacggg tacagaacat cgtcgggctg gaaggtattt
    11881 cgataccgca aggctacggt tccagttcag taccgctttt cgttctactt gatgctatct
    11941 atgaaaaaat ccctttcatg aagggacgca atatcgatgc tcaggagatc cagaaacgct
    12001 acggcatggt cggcgatccg gtgattatcg gcgtagtgct cggtctgatt tttggcctgg
    12061 cggctggtga aggctttaaa ggttgcgcca gtctgatgat taccgttgcg gcaatcatgg
    12121 tgctgttccc gcgcatgatc cgcctgattg ttgagggcct gatgcccatc tccgacggtg
    12181 caagaaagtt ctttcagaaa tatttcaaag gacgtgaagt gtatatcggt ctggatactg
    12241 ctgtgacgct ggggcatcca accaccattg ctgtcggtct gctactgata cccattatgt
    12301 taattctggc cagcatttta ccgggcaaca aagtgttacc cctcgcagac ctgccggtag
    12361 caccgttctt tatctgtatg gctacggtta tccaccgcgg agacctggtc aggacgttga
    12421 taagcggggt catcgtgatg attaccgtcc tgctgatcgc cacccagttc gcgccttact
    12481 tcactgaaat ggcgctcaaa ggcggtttca gttttgccgg tgaaagtgcg caaatctcag
    12541 cgttgtccgt gggcaatatg ttcggttggt cgatttctga attgatgtcg cttgggatta
    12601 ttggcgttgt cgtggcggtc ggtattgtcg ccagcgtcgt gctgttctta cgtaaacgtg
    12661 aattatcgga ataaggagta ctttcaatga gttggctgaa agaggttatt gggacggaaa
    12721 aagcggtcat cgccatgtgc catctacgtg cattgcccgg cgacccgagt tttgatgcgc
    12781 agttggggat gaactgggtt atcgacaaag cctgggacga tctgatggcg ctccaaaatg
    12841 gtggggtgga tgcagtcatg ttctccaacg agtttagtct tccctatctc acgaaggtgc
    12901 gtccggaaac cactgcggca atggcacgaa tcatcgggca attaatgagc gatatccgca
    12961 tcccgtttgg cgtgaatgtt ctgtgggatc cggtcgcctc attcgacctg gcgatggcta
    13021 ccggcgctaa gtttatccgc gagatattta ccggtgccta tgccagcgac tttggcgtct
    13081 gggacactaa cgtcggtgag accatccgtc atcaacaccg gattggtgca ggcgaagtga
    13141 aaaccttgtt caatatcgtc ccagaggccg ccgtgtatct gggcaatcgc gatatctgtt
    13201 ctattgctaa atcgacagta tttaacaacc atcctgatgc gctctgtgtt tctggcctga
    13261 ccgccgggac gcgtacagac agcgcgctgt taaaacgggt caaagaaacg gtgcctgaca
    13321 ccgtcgtgtt ggccaacacg ggcgtctgcc tggaaaacgt ggaggaacaa ctcagtatcg
    13381 ccgatggctg tgtgaccgca accaccttta aaaaggacgg cgtttttgcc aatttcgttg
    13441 accaggcgcg agtcagtcag tttatggaga aagtgcatca tatacgccga taagatgggg
    13501 ataagcagag cgcttatccc cggcaaagag caataaccct tctgcgatcc ctgccgcctt
    13561 cgtcaaaagt atcgacgggg atcgctacca gattaacccg aatcggcatg gagtcaacga
    13621 cggatgatta acgatattaa atgggtacag gcacaacgtg aagccaccga ctggcgtcag
    13681 gccgtggaaa tcgccacccg cccgctggta gcctatggtg ccgcgcagcc atgctacgtt
    13741 aatgggatca tcgaaaatac ccttaactgg gggccttatt atctgatagc cccaggaatc
    13801 gccttgcctc acgcccgacc agagcaaggc gcaaactata atcaggtcag tatcaccacg
    13861 ctacgcaccc cggtcgcctt tggtaacgag gagtgcgacc ccgtctggct gcttctctgc
    13921 gtcagcgcca cggatgccaa cgcacatatt ttgaccattc agcgcatcag ccagtttatt
    13981 gattcgccac aacgcttagc ggcagtggga aatgccagca cagacgatgc actgtttgcg
    14041 ctggtatcag gttaacggag gcgcttatga tcctgcaccc ttctctcgcc agcgcaaatc
    14101 ctctgcacta tggtcgtgag ctgacggcat tggacaatct ggattttgga agcctgcatc
    14161 tggatattga ggacagcagc ttcattaaca acattacgtt tgggatgaaa acggtgcagg
    14221 ccgtcgccag acagacgcct catcccttgt cctttcattt catggttgcc cggccacagc
    14281 gctggtttaa cgcgctggct gaaatcagac ccgcgtggat cttcgttcat gcagaaacgc
    14341 tggactatcc ttcggaaacg ttaactgaga tccgccatac cggcgcacga gccggactgg
    14401 tatttaatcc ggcaaccccg atcgacgcct ggcgatatct ggcgtctgag ctggacggag
    14461 tgatggtgat gaccagtgag cccgatgggc agggacagcg gtttattccc tccatgtgcg
    14521 agaaaatcca aaaagtacgc acggcttttc ctcagaccga gtgctgggcc gacggtggaa
    14581 ttacgttggc agctgcacag caactggcag cagccggggc gcaacatatg gtgattgggc
    14641 gcgccctgtt ctcttcatct gactatcgcg cgaccttagc tcaattcgcc accctgtaag
    14701 aggagattgt ttctgatgag ccaacagaga cctgaccgta tcaaacagat gctgcactac
    14761 ctgtggcaac atcgtcacct ctccacacag caggccatgg agctttttgg ctatgcggaa
    14821 gcgactgtcc gtcgtgattt ccagtatatt gccaaccagt atccaggcat gatccgcggt
    14881 cacggttgcc tggacttcga cgacagtaca gacgacaaag agtacgtctt cgacgtgaaa
    14941 cgtacattgc aatctgtggc gaaaagagaa atagctgccc ttgcccgaac aatgatcaaa
    15001 gatggcgact gcttttttct cgactccggc tcaacctgcc tcgaactggc gaaatgcctg
    15061 gccgatgcgc gggtaaaggt catctgcaac gacattaaaa tcgccaatga actgggctgt
    15121 tttccccatg tggaaagtta cattatcgga ggactgattc gcccaggtta tttttcggta
    15181 ggagagagtc tggcgctgga gatgatcaat gccttttccg tagaacgtgc gtttatctcc
    15241 tgcgatgcgc tgtcgctgga gacggggatc accaatgcca ccatgtttga ggtcggggtg
    15301 aaaacacgga ttattcaacg ctcgcgtgaa gttattctca tggccgatca ctcaaaattt
    15361 gatgccgttg agccgcatgc tgtagcaaca ctatcatgta ttaaaacgat catcagtgac
    15421 agcgggttac ctgagactat tgcacagcgt tatcagcgag ccggatgtca gttgttttta
    15481 cctcattcaa taaagtgata agtaagttcg ttcgagaggg atttcaagca aaaataatca
    15541 atggcaccca atagaaaata ttggcgatac gctcgaacga ataaagaagc cctatgcgca
    15601 atccacacgc tacgatgttg caacaacacg ccatctactt tttattctca ttcactaaat
    15661 gtggttgttc tggattcatt attcaaagtg tgtacaagat cacatttaat cacttcatta
    15721 cggttcagca tgctgaacaa agcatgtttt ccactatgta atgccgatac catttattcc
    15781 atgagcaaga aggagccatt atggttcgca aagggtgtaa ttcattagtc cgtgctgaaa
    15841 aaatattaac ccacattgca tgggttggta tggcaagtta tatggagtta ttaaataaat
    15901 tccagtaccc caaaagcagc ctgcttaatt tattgaatgt aatggtggag tgtggtttct
    15961 taattaaaaa caaaaacggc tactattcac tgggaataaa aaactacgaa ttgggttgtc
    16021 aggcgttaca ccgtcaaaat atatttgagg tgactaagcg gccaatgcag gagttgtcgc
    16081 tgaaaagtgg actggtttgt catcttgggg cgatggagag catttcggct atctatctcg
    16141 acaagatcga aagtccggac tctgtaccga cgagcaaaag ctggattggc aaaaaacttg
    16201 agttgcatat taccgcgttg ggtaaggcat tattagcctg gaagacacga gaagaactgg
    16261 attatttttt agaagcactc acgttaactc cacatacgcg caatacattt accgataaaa
    16321 aattgtttct ggaagaatta caaaaaacac gccttcgggg atgggccata gacaacgaag
    16381 aatcaaccta tggggcggta tgtttaagta tgccggtatt caatatgtat aacagagtta
    16441 actatgcaat ctcactttct ggcgatccgg tagtttattc aggaaataag atagacagct
    16501 atctggaatt gctccggaaa tgcgctgagc aaatatcata tgggctggga tacagaaacg
    16561 aaaatgagca tttacgaaaa ggaaactgag gtaatgaaaa aattcagcgg cattattcca
    16621 ccggtatcca gcacgtttca tcgtgacgga acccttgata aaaaggcaat gcgcgaagtt
    16681 gccgacttcc tgattaataa aggggtcgac gggctgtttt atctgggtac cggtggtgaa
    16741 tttagccaaa tgaatacagc ccagcgcatg gcgctcgccg aagaagctgt aaccattgtc
    16801 gacgggcgag tgccggtatt gattggcgtc ggttcccctt ccactgacga agcggtcaaa
    16861 ctggcgcagc atgcgcaagc ctacggcgct gatggtatcg tcgccatcaa cccctactac
    16921 tggaaagtcg caccacgaaa tcttgacgac tattaccagc agatcgcccg tagcgtcacc
    16981 ctaccggtga tcctgtacaa ctttccggat ctgacgggtc aggacttaac cccggaaacc
    17041 gtgacgcgtc tggctctgca aaacgagaat atcgttggca tcaaagacac catcgacagc
    17101 gttggtcact tgcgtacgat gatcaacaca gttaagtcgg tacgcccgtc gttttcggta
    17161 ttctgcggtt acgatgatca tttgctgaat acgatgctgc tgggcggcga cggtgcgata
    17221 accgccagcg ctaactttgc tccggaactc tccgtcggca tctaccgcgc ctggcgtgaa
    17281 ggcgatctgg cgaccgctgc gacgctgaat aaaaaactac tacaactgcc cgctatttac
    17341 gccctcgaaa caccgtttgt ctcactgatc aaatacagca tgcagtgtgt cggactgcct
    17401 gtagagacat attgcttacc accgattctt gaagcatcag aagaagcaaa agataaagtc
    17461 cacgtgctgc ttaccgcgca gggcatttta ccagtctgag gagaaactca tgtctgttcg
    17521 caatattttt gctgacgaga gccacgatat ttacaccgtc agaacgcacg ccgatggccc
    17581 gaacggcgaa ctcccattaa ccgcagagat gcttatcaac cgcccgagcg gggatctgtt
    17641 cggtatgacc atgaatgccg gaatgggttg gtctccggac gagctggatc gggacggtat
    17701 tttactgctc agtacactcg gtggcttacg cggcgcagac ggtaaacccg tggcgctggc
    17761 gctgcaccag gggcattacg aactggacat ccagatgaaa gcggcggccg aggttattaa
    17821 agccaaccat gccctgccct atgccgtgta cgtctccgat ccttgtgacg ggcgtactca
    17881 gggtacaacg gggatgtttg attcgctacc ataccgaaat gacgcatcga tggtaatgcg
    17941 ccgccttatt cgctctctgc ccgacgcgaa agcagttatt ggtgtggcga gttgcgataa
    18001 ggggcttccg gccaccatga tggcactcgc cgcgcagcac aacatcgcaa ccgtgctggt
    18061 ccccggcggc gcgacgctgc ccgcaaagga tggagaagac aacggcaagg tgcaaaccat
    18121 tggcgcacgc ttcgccaatg gcgaattatc tctacaggac gcacgccgtg cgggctgtaa
    18181 agcctgtgcc tcttccggcg gcggctgtca atttttgggc actgccggga catctcaggt
    18241 ggtggccgaa ggattggggc tggcaatccc acattcagcc ctggcccctt ccggtgagcc
    18301 tgtgtggcgg gagatcgcca gagcttccgc gcgagctgcg ctgaacctga gtcaaaaagg
    18361 catcaccacc cgggaaattc tcaccgataa agcgatagag aatgcgatga cggtccatgc
    18421 cgcgttcggt ggttcaacaa acctgctgtt acacatcccg gcaattgctc accaggcagg
    18481 ttgccatatc ccgaccgttg atgactggat ccgcatcaac aagcgcgtgc cccgactggt
    18541 gagcgtactg cctaatggcc cggtttatca tccaacggtc aatgccttta tggcaggtgg
    18601 tgtgccggaa gtcatgttgc atctgcgcag cctcggattg ttgcatgaag acgttatgac
    18661 ggttaccggc agcacgctga aagaaaacct cgactggtgg gagcactccg aacggcgtca
    18721 gcggttcaag caactcctgc tcgatcagga acaaatcaac gctgacgaag tgatcatgtc
    18781 tccgcagcaa gcaaaagcgc gcggattaac ctcaactatc accttcccgg tgggcaatat
    18841 tgcgccagaa ggttcggtga tcaaatccac cgccattgac ccctcgatga ttgatgagca
    18901 aggtatctat taccataaag gtgtggcgaa ggtttatctg tccgagaaaa gtgcgattta
    18961 cgatatcaaa catgacaaga tcaaggcggg cgatattctg gtcattattg gcgttggacc
    19021 ttcaggtaca gggatggaag aaacctacca ggttaccagt gccctgaagc atctgtcata
    19081 cggtaagcat gtttcgttaa tcaccgatgc acgtttctcg ggcgtttcta ctggcgcgtg
    19141 catcggccat gtggggccag aagcgctggc cggaggcccc atcggtaaat tacgcaccgg
    19201 ggatgtaatt gaaattaaaa ttgattgtcg cgagcttcac ggcgaagtca atttcctcgg
    19261 aacccgtagc gatgaacaat taccttcaca ggaggaggca actgcaatat taaatgccag
    19321 acccagccat caggatttac ttcccgatcc tgaattgcca gatgataccc agctatgggc
    19381 aatgcttcag gccgtgagtg gcgggacatg gaccggttgt atttatgatg taaacaaaat
    19441 tggcgcggct ttgcgcgatt ttatgaataa aaactgaaag cataaataaa aacatccaga
    19501 cacctgaatt atccgactct gcatcattcg ttatttgctc agtcggataa tgatgggtcg
    19561 ctaccctgtt atgagaggac gttatgccac taattatcgt tgtggcaggg attgctttac
    19621 tcctgctttt aaccataaaa attaagctta atacgtttgt ttcgttaatt attgtctcga
    19681 ttgctgtcgc catcgccagt ggtatggatt tgagtaaagt cgtcacctca gtcgaatccg
    19741 gacttggcgg tacgctgggc catattggtt tgatatttgg cttcggtgtt atgctcggtc
    19801 gcttgctggc cgatgcgggt ggtgcgcagc gaatagcgct gaccatgctg aattatttcg
    19861 ggaaaaataa acttgactgg gcagtggtct gttcggcgtt tattgtcggt atcgcacttt
    19921 ttttcgaagt cggtttaatt cttctggtac ctattttatt cgctattgcc cgtgaagcaa
    19981 aaatatcacc gatgtttatg tgtgtgccga tgctttctgg tttgctggtc gcacacggtt
    20041 ttttgccgcc gcaccccggc ccgactgtta tcgccaggga atatggcgct gatgtaggat
    20101 tggtactgat atacggtatc attgtcggca ttccaacctt tattctttgc ggcccggtat
    20161 tgaataaatt ctgccagcgc attattccgg acgcctttaa aaaagagggg aatatagcct
    20221 ctctcggcgc gacccgccga ttcagcgaaa gcgaaatgcc aggctttggg atcagttttc
    20281 tcacggcaat gctgccggtg atcctgatgg cggttgtcac catcatccag atgactcatg
    20341 ccaaaagtgc cgcggattca ggcttatttt acaacgtgat actgttctta ggtaattcga
    20401 cgatcgccat gctgatttca ctgctgttcg cgatctatac catgggcctg gggcgcggga
    20461 aaacaatccc tgacctgatg gattcttgcg gaaaagctat cgcgggtatt gccggtctgc
    20521 tgctgattat cggtggtggt ggtgcattca agcaagttct catcgactct ggcgttggtc
    20581 agtatatttc caccctggtt tcgggtatgg atattaaccc gatcctgatg gcctggggcg
    20641 tggccgcatt cctgcgtatt tgtctgggtt cagccaccgt cgcggcgatc tctaccgccg
    20701 gtctggtcat cccattgttg gcagtacatc ccaataccaa cctggccttg attacgctgg
    20761 ctaccggtgc cggatcctgt atttgttctc acgttaacga tgccagtttc tggatgatta
    20821 aagacttttt tggcctgacc accaaagaaa cgctgttatc ctggacgctg atgtccacac
    20881 tcttatccat cagtgggctg atttttattc tgctcgccag cttagtttta taatcgggtt
    20941 cttgccagtg agccaacctg ttgttcactg tcccccataa tttagtgagt gacggcaacc
    21001 gtctgcacgg tgacgaatcg ctggcgatca aaaaggggcg atattgaacc gtcgattatg
    21061 cttgatgggt gtactctcag taatactgta aagccggagc atgtctccgg ctttacagta
    21121 tgggggaaca gcgggcgatc gctcaaatgg tatgctggtt tatttcatgt aaaagaataa
    21181 aatatctgta taaaatcatg ccactggcaa tctgcatcaa ctctccgggt aaacagggtt
    21241 aagatgagca tttccccggt ttaatacagg tatacaaaag tggatagaga tcaaacaaac
    21301 agcagcctgt tcaatgatga tcccgtactt catgcaacat ggctgtacta tcaggaaggc
    21361 aaaagccaga ctgaagttgc agctatcatg ggggtgtccc gcgtcacggt tgtcaaatac
    21421 ctgcaaacag cccgggaaaa tggactggtt catatcaacc tagatgtgaa tgtctttggc
    21481 tctatcgacg ctgctttgca aattcgcgat aaattcaatc tccagagagt gatcatcgtg
    21541 ccggatggcg aacatgccgg aaaacgtgat gatacaaagc tgatgcggac tcgcctttca
    21601 cgtgccggtg gcatgtatct gaatcaggtg attgagaatg gtgatgtgct gggggttgcc
    21661 tggggaagaa caatccatca gatgagcaaa accatgacgc cgaagtcatg taaaaatgtc
    21721 accgttatac agatgctcgg ttccatgcca tctcagccag acctgacgat cattgaatcc
    21781 tcatcgcaaa tcgcctacaa gctttcaggc cgtgtcgcct cgctgcacgt gcccgccgtg
    21841 gtatcaagcg ccagactggc catggagtta caggcagagc ctattattcg atcgaatttt
    21901 gacgttctga ctcgctgtac gaaagccttt tttgtcgtgg gtaacgcgct tgatgaaaat
    21961 cccctgattc gggtgggcgt gctgaataag aaggaaatgc agacgtatcg ggatttaggc
    22021 gcagtaggtg tcatttgtgg tcgcttctat gacaaggaag ggatgcccgt cgtggctgat
    22081 gttgaccagc gtattctggg aatcagtctg gctcagctaa ggcagattga acggaagatc
    22141 ttccttgctg gtggggaaag aggctatgac gcaacgttgg gtgccctgct gggaggatat
    22201 gtcacggacc tgattgttga tgaaggaacg gcagagtttt tgctggcgtg tgaattacca
    22261 cactaaaaga atgaacaact gccccatgta gatatggggc aggattttat tgattagcgg
    22321 cgataataag aacaataccc acaatgtatg aaataaacat ggtcagcagc agtttattgg
    22381 ttccggctgg cgcactggca aattctctcc agatgaagac tccccagatc acggcaatca
    22441 ttgtggcccc ctgtcccaga ccgtaggaaa tggcataccc cgcctgacca gacgcaatca
    22501 gactgaatgc cagaccgaca caccaaatca taccgcccag ccagccgcaa acatgatccc
    22561 gtaaactccc ggaaaaatac attttcccgt tgaccggttc tcctgaaata ggttttttca
    22621 tgaccagcct gttaaggaca aagttcgaca aaaacagccc cacggcaaac agaacaagtg
    22681 ctgagtaagg cgtcatcaga ccgctggcag gctggctgaa attgtcagac atggagtccg
    22741 ccaggaagcg gaaaaaccat cccatgatga tacccgccag aatggcagta atgagacctt
    22801 tattacggcg agatttatcc gcctcctgcg tgacacgacc ataggctatc gcggtaaaaa
    22861 taatggcgat cacaacgcag acgacaccga ggaacagaat gagcggatcg ccctgcggat
    22921 tgccgatata ggtggtgatc acacccagag ccagtgccag ccccacaccc acaggaaaag
    22981 ctaccgccat accggcaaga ttaatagaag cgaccagaag aatattggaa atattgaaga
    23041 taatccctgc cagtattgcc gacatcagac tactgctgct cgcctgctga atatcaggta
    23101 taaaactgcg accggcttcg cccatagaac ccagcgtgaa ggcaaataac aaagaacaca
    23161 gcaataatcc aattgaataa tcccaataga atagagggaa ctcccatttt ttattgctga
    23221 caagtttagt tgtattcgcc catgaacccc agcaaatcat ggtaataaaa cacattatta
    23281 tggcgacagc atacgattcc acgataaaca taaataactc cttgtggtag ttataatcaa
    23341 tcgttgtgga cagagggaat agcagccatt ctgcgaatgg ctgtcatatg ttattaatta
    23401 aaataaagct gtaattacgc tttcaaacca tacgttttga atttttcaag gacgatggtc
    23461 atttcggctt cagacagcac gggaacatcg tgatgtaatc cgccacattt gatgtataaa
    23521 tcagccagcg tctcggtttc acccgccagc cacaacgtct tttccagcgt gacgtccatg
    23581 gccagcatac cgtggtgctg catcagcaag gacttacttt ctctgattcc tttagataca
    23641 ccgtcggcca gttcaggact gccaaaggta taatagggaa tacagggaat atgatccgtc
    23701 cccgataccg ccaccatata atgaatggcc ggaattgaat gattatgtat tgccaccgcc
    23761 gtggaattca ccgcatgcgt atgtatgaca gcattcaatt ctggtctggc tttataacac
    23821 tccagatgaa ttaaccattc acttgatggt attttacccg cttcaggttt tcccttatcg
    23881 tctacaaaga caatcatgtc cggcgtcatc tttgaataag cgatcccact cggcgtaatc
    23941 aacatgccac cggtatacct ggcactcaca ttacctgatg ttccttgatt aagacctgac
    24001 gcattcattt cccgacaagt ggaaatgatt ttttctgcta acttaatccg ttccattcct
    24061 gatccttaat agcaatggcc gctgttttaa tgctgttccc cctggagtgc tttactcttc
    24121 aggtcagcaa acatatccgc cagcgctgac ttactggcag cacagactcc ccgctcagta
    24181 atgaagccgg taatgtatcg ggcaggcgtg acgtcgaaag cgtagttccc acaacgggtt
    24241 ctttcagggg cggtattgac ccagctcaat tctccctgag ggtttatccc atagacatgg
    24301 gactgctctt ttccgtcacg ttgctcaatc ggaatgcttt ttccgtcctc gatagtccag
    24361 tcaatagtgg gagaaggtaa cgcgacgtag aagggaacat ggttatcatg ggccgccagc
    24421 gccttaagat aggtaccaat tttattacag acatcccctc tggccgtggt tcgatcggtc
    24481 ccaacaatgc agagatcaac gtcaccgtgt tgcatcaggt ggccacccgc gttgtcggcg
    24541 atcagggtgt gaggaatacc gtgtgagccc agttcaaatg ccgtgagtcc gccctgatta
    24601 cgtggccgcg tttcatccac ccagacgtga acagggattc cgttttcatg cgctttgtag
    24661 atgggagaaa gcgcggttcc ccagtctaca gtcgccagcc agcctgcgtt acaatgtgtc
    24721 aggatattga ccacactccc ggccggtttt ttctgggcta tttcgcggat gacttctgca
    24781 ccatgtaatc caatttgttc acacaacgcg acatcttcct cggcaatttc tgccgccagc
    24841 gcccaggcca catccttgcg ctctgataca cagagatcct tcagtgtgtc tatcatcctg
    24901 tcgagggccc atttgagatt aattgccgtt ggtcgggttt tgataagaag gtcatagtaa
    24961 cgctgcagcc ccatgtcact ggcatcgtga ttcattccca gcgcgatgcc ataggctgca
    25021 acgacaccaa taagcggagc gccacgaacc cacatatcct gaatagccgt agcggccatt
    25081 tccgcagagg taagcgccac cacctcgaac ttaaaaggca acttagtctg atcgatgatt
    25141 tctaccgctt ttccatcccc ggaaacccag accgtacgat agtgtttacc tttaatattc
    25201 ataatgacct cttacttaac cttcgcagac tggatgacgg aatccatatt tttaaactgg
    25261 cgggcattga cgataagatc gcgcgccatg gtcaatgcac gtcgttcaca tcctgcgcga
    25321 agctctgtat tttcaatctg tttcagctcg gcaacgccag caaaaccaat gatccggcga
    25381 ttcatttcca tgccggcatt caccagggaa tcttcaagca gcgtggcaaa aaactgatcc
    25441 tgtgcgtcct caagagcggc cctgttctgt tgatacattt ctgtcgacca cgcgtcgccc
    25501 tgtgttttgt tgtcccagag ctggcggaac tcccgggtga aaacggacca ggtttggaca
    25561 atctgctgaa gcagccattc ctgatagtca gcgcgacgtt gctcattcgc atcccaccca
    25621 gggcgtgaga agtacgccag caggaggttg ccaatatagt tgccgatatc aaacgccatt
    25681 ggccccatga aaccgaactc cggatcaatc actttggtat ccgtgtcggt caccatgatt
    25741 gagcctgaat gaaggtcgcc atgtaacaat gcctgcgcct ctgtcataaa tttgtactta
    25801 taacgcatgg caacctggat catctctaca tcagcccagg ccttatggac ggcatcgtcc
    25861 agctcaggag aagtccagtt attccgttca gcgttgtaat agggctctgt gaagatcaga
    25921 tcttccgtaa ttttgcacag ctcatgattc aacgcgaact cggcagtaag cgcttttttc
    25981 tgttctgctg acatgccaat gtcagacgta ttgaagagag tctgtgctaa aaatctgccg
    26041 atatcttcag ctagtttagg gaatttttta ccgttaatca gttccttacg cagaatcacg
    26101 tgaggagtca gatactccat ggcaaacagc gccatttccg ggtcgtaaaa atagacctgc
    26161 ggaatcagtg tgtgacccgc gtacttggct tcctgacata agacgttgta ctcaaagccc
    26221 gcacggctca gagataaagg ccatccttcg cccccggcac gaagccaggg cagagcttgt
    26281 tttacaatga tggttttatg tgtcccctca acgataaaca ccatgttcag gtttccgtcc
    26341 cccacttctg aaactttcca ctcttcaggt aaccctcccg gttcgcatga aggggtcagt
    26401 ctggacgaga gataacccgg cagcgtgtca catgtcagcg gcttgtaacc tgatggaatt
    26461 gaatccgtca taacagttct ccttgtctac acagaatgat aatcaacttg tcatgtgaca
    26521 actatacatt atcaaatgta aaattatttg tgacgcatgt agattttttg aactgtataa
    26581 aattaaaaat gatgtcgtat ggagtcatga tccttacagg aatcagcaga ggaaaacatc
    26641 tattattacc atgtaattca tgttgttatg tcgcaacaat agtactgcac ggggaattat
    26701 tacgggcaaa cttaggcgaa aggaagtact gaagtgacag aataaatcac actttttgta
    26761 catatgtact tttctattgt cttttgttgt ggaaacaaat ggtgtttgtc atgccgatgc
    26821 taatagacag atagattcga gggccaacag ccccatcatc atgatggagc aacagaggtg
    26881 agtctaacgt ttccgcactt cttatcgatc ggcactaacg atcaatatcg ataaactgat
    26941 ctatcaaaac gattatcttt cttgcaacct gcctggttat ccttctctta cattgctcgc
    27001 agaaaagccc ttttattacg gttagttatc tagctgaggc ttgctacgcc cattttccat
    27061 gtaccccaaa tcacgaggtc acaaaggcgc ggaaagatga agggatgtgc acgttgcaac
    27121 aaaaaaaaga agcgtccttg aacagaggct ctcactcgat aaagaattct gcgacgagtg
    27181 cttattcgat atcgatgagg aaatcaggga atgctggtcc agtaaccggg tgagcagtgt
    27241 ctgccagatg gtgacgggag acgggcagca cgatacctcc cgtacatgcg tgtcgggtaa
    27301 tgttttcata ctgaagattt tcctgatatg cagagataaa aaatgggaca gtggcgtggt
    27361 gaaaacacca ggccggagca gaaggttatt ctggtggttt actttttcgt ttcaggtgtc
    27421 ggataaacag ccagataaac gtaaccacag gagccgaggg tgtcggcttc acaggtcagc
    27481 cccttcgcat acagtgtcac ggtatgctga tggcggggat tcagttcacc actggtgagc
    27541 attaatttca gttgtttcat cagcagcgga aaggcctggt ccagagggta cgcatccgca
    27601 tctctgaacc ggcctctgat accggcacgg tcggcaaggt aatgcaaccg gttaccctcc
    27661 tgcaccagac gggcgccgaa acagggcgtc actgtgcagg gtagtcccca ccagggatgg
    27721 tcgtcattgt catcgggatg cgttgtcccg gaaagtgtgt ctgacacgat aaaatcctca
    27781 cagaaaatcg gtgaatgata gtttaacgat gacgggtcag ccggcgcgca gaccggcttc
    27841 ggtaaaggtg tattcgttga tgagcaggac ctcatccaca gcggcgtctg agctcagcca
    27901 gtcgtactcg ttttccgggt gacggaacat cagttcaggg tgacgctcat cagtcggaca
    27961 tacggaccaa aactgtcctt acggcgttct gcaaacacgg ccagcacacc gggaatatcc
    28021 tgcacttcac gaccggtata cgcttcagca ctgccgtgcc agcggtattt gccggtacag
    28081 aacggaaaaa gacgggatgc cggatgctga cggtgaatac gcatggcttc gccacgggtg
    28141 atgattttca tagtgggata cctctgaaga cagaagataa aagtgaaaac aggtgtgatg
    28201 tggttgtgac gggttaaagc agaccatatt cggcaaagga gaaaacctgg ctgccaccga
    28261 ctatcagatg gtccggcacc cggatatcca ccaggcccag tgcctgtacc agacgttccg
    28321 tgataaggcg gtctgcctta ctgggggtga cttcaccgga cgggtgattg tgtgccagca
    28381 ccacggctgc ggcattgtg
//
LOCUS       DADTFG010000058        28098 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.058, whole genome shotgun sequence.
ACCESSION   DADTFG010000058 DADTFG010000000
VERSION     DADTFG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.058"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            10..86
                     /locus_tag="J1138_002233"
     tRNA            10..86
                     /locus_tag="J1138_002233"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44..46,aa:Met,seq:cat)
     gene            135..209
                     /locus_tag="J1138_002234"
     tRNA            135..209
                     /locus_tag="J1138_002234"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:167..169,aa:Gln,seq:ctg)
     gene            complement(363..1538)
                     /gene="ubiF"
                     /locus_tag="J1138_002235"
     CDS             complement(363..1538)
                     /gene="ubiF"
                     /locus_tag="J1138_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBA8454029.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            1684..3108
                     /gene="miaB"
                     /locus_tag="J1138_002236"
     CDS             1684..3108
                     /gene="miaB"
                     /locus_tag="J1138_002236"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBA8454030.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPELVIARTRKENDLGVGYYQP"
     gene            3222..4301
                     /locus_tag="J1138_002237"
     CDS             3222..4301
                     /locus_tag="J1138_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBA8454031.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4298..4765
                     /gene="ybeY"
                     /locus_tag="J1138_002238"
     CDS             4298..4765
                     /gene="ybeY"
                     /locus_tag="J1138_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBA8454032.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            4855..5733
                     /gene="corC"
                     /locus_tag="J1138_002239"
     CDS             4855..5733
                     /gene="corC"
                     /locus_tag="J1138_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBA8454033.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5758..7296
                     /gene="lnt"
                     /locus_tag="J1138_002240"
     CDS             5758..7296
                     /gene="lnt"
                     /locus_tag="J1138_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBA8454034.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVTVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7423..9300)
                     /locus_tag="J1138_002241"
     CDS             complement(7423..9300)
                     /locus_tag="J1138_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBA8454035.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQAGIDNACHIGGLIAGGALGWLSARLVGQNRFVTEGGIIV
                     AVTLLLTGAIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALTET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGNARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADDRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            9754..10662
                     /gene="gltI"
                     /locus_tag="J1138_002242"
     CDS             9754..10662
                     /gene="gltI"
                     /locus_tag="J1138_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBA8454036.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSNTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10832..11572
                     /gene="gltJ"
                     /locus_tag="J1138_002243"
     CDS             10832..11572
                     /gene="gltJ"
                     /locus_tag="J1138_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBA8454037.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            11572..12246
                     /gene="gltK"
                     /locus_tag="J1138_002244"
     CDS             11572..12246
                     /gene="gltK"
                     /locus_tag="J1138_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBA8454038.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12246..12971
                     /gene="gltL"
                     /locus_tag="J1138_002245"
     CDS             12246..12971
                     /gene="gltL"
                     /locus_tag="J1138_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBA8454039.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIIEDS
                     PKGAFFDNPKSDRAKDFLAKILH"
     gene            13089..14024
                     /gene="rihA"
                     /locus_tag="J1138_002246"
     CDS             13089..14024
                     /gene="rihA"
                     /locus_tag="J1138_002246"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBA8454040.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(14069..14551)
                     /locus_tag="J1138_002247"
     CDS             complement(14069..14551)
                     /locus_tag="J1138_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBA8454041.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            14786..17368
                     /gene="leuS"
                     /locus_tag="J1138_002248"
     CDS             14786..17368
                     /gene="leuS"
                     /locus_tag="J1138_002248"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBA8454042.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVKDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTEMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDVLTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            17383..17964
                     /gene="lptE"
                     /locus_tag="J1138_002249"
     CDS             17383..17964
                     /gene="lptE"
                     /locus_tag="J1138_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBA8454043.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTLATPARVSTTLGN"
     gene            17964..18995
                     /gene="holA"
                     /locus_tag="J1138_002250"
     CDS             17964..18995
                     /gene="holA"
                     /locus_tag="J1138_002250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBA8454044.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            18997..19638
                     /gene="nadD"
                     /locus_tag="J1138_002251"
     CDS             18997..19638
                     /gene="nadD"
                     /locus_tag="J1138_002251"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBA8454045.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSAQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            19662..20273
                     /locus_tag="J1138_002252"
     CDS             19662..20273
                     /locus_tag="J1138_002252"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBA8454046.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            20533..20850
                     /gene="rsfS"
                     /locus_tag="J1138_002253"
                     /gene_synonym="rsfA"
     CDS             20533..20850
                     /gene="rsfS"
                     /locus_tag="J1138_002253"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBA8454047.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            20854..21321
                     /gene="rlmH"
                     /locus_tag="J1138_002254"
     CDS             20854..21321
                     /gene="rlmH"
                     /locus_tag="J1138_002254"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBA8454048.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            21352..23253
                     /gene="mrdA"
                     /locus_tag="J1138_002255"
     CDS             21352..23253
                     /gene="mrdA"
                     /locus_tag="J1138_002255"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBA8454049.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            23256..24368
                     /gene="mrdB"
                     /locus_tag="J1138_002256"
                     /gene_synonym="rodA"
     CDS             23256..24368
                     /gene="mrdB"
                     /locus_tag="J1138_002256"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBA8454050.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            24379..25467
                     /gene="rlpA"
                     /locus_tag="J1138_002257"
     CDS             24379..25467
                     /gene="rlpA"
                     /locus_tag="J1138_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBA8454051.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            25607..26818
                     /gene="dacA"
                     /locus_tag="J1138_002258"
     CDS             25607..26818
                     /gene="dacA"
                     /locus_tag="J1138_002258"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBA8454052.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            26928..27191
                     /locus_tag="J1138_002259"
     CDS             26928..27191
                     /locus_tag="J1138_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBA8454053.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            27292..27933
                     /gene="lipB"
                     /locus_tag="J1138_002260"
     CDS             27292..27933
                     /gene="lipB"
                     /locus_tag="J1138_002260"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBA8454054.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         6836 a         7332 c         7235 g         6695 t
ORIGIN      
        1 aaacaatctg gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac
       61 aggttcgaat cccgtcgtag ccaccaaatt ctgaatgtgt cgaatatgtt cggcaaattc
      121 aaaaaccaat ttgttggggt atcgccaagc ggtaaggcac cggattctga ttccggcatt
      181 ccgaggttcg aatcctcgta ccccagccac attaaaaaag ctcgcttcgg cgagcttttt
      241 gcttttctgc gtatattcaa tgccgaatgc gatgttgact cgtcttatcc ttcaaagtcg
      301 gatgcgacgc tgccgcgtct tatccgacct acggttggca tgcatccagc aatgttgtga
      361 ggctacaacc ctaacgcata tttcagcgcc ttacgtttca acacgccagc acgctccgcc
      421 gccatcaaac caagattacg cacaaaacgc agcggtggca gattattgct gaatccggca
      481 taaaacagat ccataccact ttgcatcatg aagttatccg ccatacgccg catctggtaa
      541 cgcttgagca caggataact ggcccacgct tcgccgtagc tgcgggcgtt caccagaacg
      601 tcaatcaggg catcgacatc acgataacca agattcactc cctgtcccgc cagcggatgg
      661 atggtatgcg cggcatcgcc caccagagca agccctggtt gcacatactg caacgcatgg
      721 cgacgtatca gcggaaacgc accagcggca agcggtgtca cgtaacccag acgcgacggg
      781 aaatgcttcg cgatttccgc ctgtagctgc gccatattca tattctgcaa ctggcgaatg
      841 cgcgccggag agtcatacca caccagcgat gcccagttat caaacaacgg cagaaacgca
      901 cgcggtccgt ccggggtaaa ttgctgccag gtgctgtcgc cgggatcgtt ctcacactgt
      961 acgctaatca acatacacga ctgcgcatac tgccaggcat gaacgccaat tcccgccatc
     1021 tgtcgcacct gcgaatttgc gccgtcggca ccaatcacca gcttcgcgag gatcgtttca
     1081 ccgcccttca gttccagctc ctgaagatca ttatggcgat gcagtgcaat cagcgaggtt
     1141 ggcacacgta acgttacttt cggatgcgct tccagcgcct gccacaacgc ctgttgcagg
     1201 acagtgtttt ccaccatgta gccaagcaac ggtagcttaa gttcagcggc gtcaaacacc
     1261 acatgcgccg tttcccactc ccacgtttcc agtctgcggt aaggatggca acgcatagcc
     1321 tgtactgcat cccagacccc taacccttta agcaatgata ccgaagccgc actaatcgcc
     1381 gagatccgca cgtccggctg gctgtcagcg acaaacggtg caggttgggc atgttcgata
     1441 accattaccg aaaatccgtg ctgagccagt cccagcgcca gtgcgccgcc gaccattcct
     1501 ccgccgacaa tggcaatttc cgttggttga tttgtcatgg tcaatcatcc tgttagcaat
     1561 atgctttaag tttacaggat ttttaccccc aagggctgat aacgctcata ctggtcacaa
     1621 cggcagcaaa gcattacact atgcgccctg cattcctggc tactatttcg caagagcaag
     1681 tcgatgacca aaaaactcca tattaaaacc tggggctgtc agatgaacga gtacgattca
     1741 tcgaagatgg ccgatctgct ggatgccacc cacggctatc aactgaccga cgtggcggaa
     1801 gaagcggatg tgctgctgct gaacacctgc tcaatccgcg agaaggctca ggaaaaagtc
     1861 ttccatcagt tgggtcgctg gaaactgtta aaagagaaga atccagacct gattatcggc
     1921 gtcggcggct gcgtggcatc gcaagaaggc gagcacattc gccagcgcgc ccactatgtc
     1981 gatattattt ttgggccgca aacgctgcac cgcctgccgg agatgatcaa ctccgtgcgc
     2041 ggcgaccgca gcccggttgt agatatcagc ttcccggaaa tcgagaagtt tgaccgtctg
     2101 ccagaaccgc gcgccgaagg gccgaccgcg tttgtctcca tcatggaagg ctgcaataaa
     2161 tattgcacct actgcgtggt gccttacacc cgtggtgaag aggtaagccg tccgtccgac
     2221 gatattctgt ttgagatcgc ccagcttgcg gctcagggcg tgcgtgaagt caacctgctc
     2281 ggtcagaatg tgaatgcctg gcgtggtgag aactacgacg gcaccaccgg aacgtttgcc
     2341 gatctgctgc gtctggttgc cgccatcgac gggatcgatc gtattcgctt taccaccagc
     2401 catccgatcg aattcaccga cgatatcatc gaagtgtatc gcgacacgcc ggagctggtg
     2461 agcttcctgc acctgccggt acagagcggt tccgatcgca ttctgaacct gatgggccgt
     2521 actcacacgg cgctggagta caaagcgatc atccgtaaac tgcgtgcggc gcgtccggat
     2581 attcagatca gttctgactt catcgttggc ttccctggcg aaaccaccga agacttcgag
     2641 aaaacgatga agctgattgc cgacgtcaat ttcgacatga gctacagctt tatcttctct
     2701 gcacgtccgg gtacaccagc cgccgatatg gttgatgatg ttccggaaga agagaagaag
     2761 cagcgtctgt atattctgca agagcgcatt aatcagcaag cgatggcatg gagccgccgg
     2821 atgctcggca ccacccagcg cattctggta gaagggactt cacgtaagag catcatggag
     2881 ctttccggtc gcactgaaaa taaccgcgtg gttaacttcg agggcacgcc ggatatgatc
     2941 ggtaaattcg tcgatgtaga aattaccgac gtctacccga actctctgcg cggtaaagtg
     3001 gtgcgaactg aagatgaaat gggtctgcgc gtggcagaaa caccggaatt ggtgattgcc
     3061 cgtacccgca aagaaaacga ccttggcgtg ggttattatc agccgtaatt ctcaggcccg
     3121 ccgttccggt gggccttgtt ttttctctct ttatccccat ctttatcgca atgcttgcgc
     3181 ctttttcctg tggcgtgaat aatttcctta taggccggtg aatgttcagc ttccggcggc
     3241 atacagataa gaggaacggt ttgaacatag acactcgcga aatcaccctg gagccagcag
     3301 acaatgcgcg tctgttgagc ctgtgcggcc cgtttgatga caacatcaag cagctcgaac
     3361 gccgtctcgg catcgagatc aatcgccgcg ataaccactt taaactgacc ggccgtccga
     3421 tttgcgtcac cgctgcggca gacattctgc gtagcctgta tgtcgatact gccccgatgc
     3481 gtggtcagat tcaggatatc gaaccggaac agatccacct tgcgattaaa gaagcgcggg
     3541 tactggagca aagcgcggag agcgtgccgg agtacggcaa agcggtcaat atcaagacca
     3601 aacgcggcgt aattaagccg cgcacgccaa accaggcgca gtacatcgcc aatattctcg
     3661 accatgacat taccttcggc gttggcccgg cgggtacggg taaaacctac ctggcagtgg
     3721 ctgcggcagt tgatgccctg gagcgtcagg aaattcgccg tattctgctg actcgtccgg
     3781 cggtcgaagc cggtgagaaa ctgggcttcc tgcctggcga tttaagccag aaagtagacc
     3841 cgtatctgcg cccgctgtac gacgcgctgt ttgaaatgct gggctttgag aaagtcgaga
     3901 aactgattga gcgcaacgtt attgaagtcg caccgctggc ctatatgcgt ggtcgtacgc
     3961 tgaacgacgc gtttatcatt cttgatgaaa gccagaacac caccatcgaa cagatgaaga
     4021 tgttcctgac ccgtatcggt tttaactcaa aagcggttat caccggcgac gtcacacaga
     4081 tcgacctgcc gcgtaatacc aagtctggtt tgcgtcatgc catcgaagtg ctggccgatg
     4141 tcgaagagat cagctttaac ttcttccaca gcgaagacgt ggttcgtcac ccggtggtgg
     4201 cgcgtatcgt taacgcctat gaagcctggg aagaagccga acaaaaacgt aaagcggcgc
     4261 tggcagcaga acgcaagcgc gaagaacagg aacaaaaatg agtcaggtga tcctcgattt
     4321 acaactggca tgtgaagata attccgggtt accggaagag agccagtttc agacatggct
     4381 gaatgcggtg atcccgcagt ttcaggaaga atcggaagtg actattcgcg tggtcgatac
     4441 cgccgaaagc cacagtctga atctgaccta tcgcggtaag gataagccga ccaacgtgct
     4501 ctccttcccg tttgaagtac cgcctggcat ggaaatgtcg ctactgggcg atctggttat
     4561 ctgccgtcag gtggttgaga aggaagctca ggagcaaggc aaaccactgg aggcgcactg
     4621 ggcgcatatg gtggtgcacg gcagtctgca tttgttaggt tacgatcaca tcgaagatga
     4681 cgaagcagaa gaaatggaag ccatcgaaac agagattatg cttgctctgg gctatgagga
     4741 tccgtacatt gccgagaaag aataatttgc caggcatttg actggcagac gtccccgccg
     4801 ttggtgatta acgacggcgt tttgattaac taacatgaga acccatacga cgccatgagc
     4861 gacgacaatt cacacagtag tgacacgata agcaacaaga agggattttt ctccctgtta
     4921 ctcagccaac ttttccacgg tgaaccgaaa aaccgtgacg aactgctggc gctgatccgt
     4981 gattccgggc agaacgacct tatcgacgaa gatacgcgcg atatgctcga aggggtgatg
     5041 gacatcgcag accaacgcgt tcgcgacatc atgatccccc gctcccagat gattaccctg
     5101 aaacgcaacc agacgctgga cgaatgcctt gatgtcatca ttgagtccgc ccactcacgt
     5161 ttcccggtga ttagcgaaga caaagatcac attgaaggga ttctgatggc gaaagacttg
     5221 ctgccgttta tgcgcagcga tgctgaagcc ttcagcatgg acaaagtgtt acgtcaggcg
     5281 gttgtcgttc ctgaaagtaa gcgcgtagac cggatgctga aagagtttcg ctctcagcgt
     5341 taccacatgg cgatcgtcat tgacgaattc ggtggggttt ccggtctggt gaccattgaa
     5401 gacatcctgg aactgattgt tggtgagatt gaagacgagt atgacgaaga agatgatatc
     5461 gacttccgtc agctgagtcg tcatacctgg accgtgcgcg cactggcttc tattgaagac
     5521 ttcaacgaag cgttcggcac ccactttagc gatgaagagg ttgacactat cggtggtctg
     5581 gtgatgcagg catttgggca tcttccggcg cgtggcgaaa ccatcgacat cgacggttac
     5641 cagttcaaag tggcgatggc cgacagtcgg cgtattattc aggttcatgt caaaatcccg
     5701 gatgactcac cccagccgaa gctggatgaa taaaaccgaa actggataga taactacatg
     5761 gcttttgcct cattaattga acgccagcgc attcgcctgc tgctggcgtt attattcggt
     5821 gcctgcggaa cgctggcctt ctctccttac gacgtctggc ctgcggcgat tatttcgctg
     5881 atggggcttc aggcgttgac ctttaaccgc cgtccactcc agtctgccgc tattggcttt
     5941 tgctggggat ttggcctctt tggcagcggt attaactggg tctatgtcag catcgcgacc
     6001 tttggcggaa tgcctggccc ggttaacatc ttcctggtgg tactgctggc ggcgtatttg
     6061 tcgctgtata ccggactgtt tgctggcgtg ctgtcgcgtc tgtggccgaa aaccacctgg
     6121 ctgcgcgtgg cgattgccgc ccctgctctc tggcaagtga ccgagtttct gcgcggttgg
     6181 gtgctgactg gcttcccgtg gttacagttc ggctatagcc agattgatgg cccgttaaaa
     6241 gggctggcac cgataatggg cgtggaagcc attaacttcc tgctgatgat ggttagtggc
     6301 ctgctggcac tggcgttggt caaacgcaac tggcgtccgc tggtggtgac cgtcgtgctg
     6361 tttgcccttc ccttcccgct gcgttacatc cagtggttta ccccgcaacc ggagaaaacc
     6421 attcaggttt cgatggttca gggcgatatt ccgcaatcgc tgaaatggga cgaaggccag
     6481 cttcttaata cgctgaagat ttactacaac gcaacggcac cgctgatggg caaatcatcg
     6541 ctgattatct ggccggagtc ggcgataacc gatctggaaa ttaatcagca accgttcctt
     6601 aaagctctgg acggtgagtt gcgcgataaa ggtagctcgc tggtcaccgg gattgtcgac
     6661 gcgcgtctca ataaacagaa ccgctacgat acctacaaca ccatcatcac actgggtaaa
     6721 ggcgcaccgt acagctatga atcagccgat cgctataaca aaaaccatct ggtgccgttt
     6781 ggcgagtttg tcccactgga gtcgatactg cgtccgttag caccgttctt tgatttgccg
     6841 atgtcgtcat tcagccgcgg accatatatt cagccaccgc tgtcgttgaa tggcattcaa
     6901 cttactgcgg ccatttgcta cgaaatcatt ctcggcgagc aagtgcgcga taacttccgc
     6961 ccggataccg actatctgct gaccatctcc aacgatgcgt ggtttggtaa gtctattggt
     7021 ccatggcagc acttccagat ggcgcgaatg cgtgcgctgg agctggcgcg cccactgttg
     7081 cgcagcacca acaacggcat tacggcggtg attggcccgc agggtgagat tcaggcgatg
     7141 atcccgcagt tcacccgcga ggtgttgacc actaacgtga caccaaccac cggactcaca
     7201 ccgtacgcac gaaccggcaa ctggccgctg tgggtgctga cggcgctgtt tggttttgcc
     7261 gctgtactga tgagtctgcg tgctcgcaaa cgttaatgtg cctgatgcgc gacgcttatc
     7321 aggcctacgc gatttttaca atatgttgaa ttttcgcgat tttgtaggat aaagcgtact
     7381 ttttcaacac gctctggggc atgattatca tgcccttttt tcttacggtt cagggataca
     7441 agccacatcg ccatattggg gatgccgaat aattgcctgc ggatcaagcg agcgcgtcct
     7501 gtccatttgg attaacctgt tcttcgccat aacaaatatc tttctatcat ccgctgaaaa
     7561 acgaagatga ttcacgtagc tggcgggata aacgccaata atctgcccgg cattaatatt
     7621 gagcaggatg acataacctt gcaggccata tgacaccaac gctaaatact gaccgtccgg
     7681 gctaataccc tcaacggaat aaacgctcct gctacaccat tgcgtcataa tgctggcttg
     7741 ttgatcctca gctcttatac gtgaaatccc ctggctatcg gcaaaaataa tctcaccatt
     7801 ttttggtcgg aatcccatcg cagaaacgac accacgcgca ttacctgcgg gaaagtcttc
     7861 tttctgatgc gtgatggtat tgatccgcac gatgttacct ctgcctgctc ccggaaccag
     7921 taatgttttt tcatccgggg aaagtgccat aacccaggcg cctggtttac cgctcatccc
     7981 cgtcccgtcg tatttctcca gcgggatatc cgccacatgc tgataagcca ccagatcaat
     8041 cgctgacaca ctattactgg tgcgattcat cacccaggct tttgtgccat cacgcgagag
     8101 gataaagctg tcaggatgtg cgccggtggt aatcgactga ataatctccc ccgtcgccac
     8161 gttaatgacc gataacgcat tcgcttcaaa tgaagtcgca taaatcaact tttcatccag
     8221 gcttaatgcc agcgatctga cgccctgatc tttacagcca ccacaatgtt tgactgactt
     8281 ttctttcgct atgggcgctg taaaggtatg caggattttc ttttgtccga ggtcgaaaac
     8341 aacaatgctg ttcgtgtttt caattgcggc atacaacgta ttaccatccc gactgatcgc
     8401 catatcatgg atatcggcaa tctcagcgag taaatcacca ctggcctgct cacgacttac
     8461 cggagcggat aatgtttccg tgagggcgtt gcgctcctcc actaactgct gctttttttg
     8521 tcgtcgctct tgttcaatct cctgcggata aaacgcttca cgcaggcttt gcctgacctg
     8581 taaaactgat tcatcaatct gctgctgcac aagccagata gccccggtca gaagaagagt
     8641 gaccgcaaca ataatcccgc cttccgtaac gaatcggttt tgcccaacta aacgcgcgct
     8701 cagccaaccc aacgcgcccc ccgcaatcag gccgccaatg tggcaagcgt tatctattcc
     8761 tgcttgccgg gtaccataca acagtgtcag cgccaccata cccaacagag gaaatactcg
     8821 ccgctggttt ttatggtgtg tgcccgcacc tgaagcaagc tgtgttgcta tcgcagcgcc
     8881 cgctattccc ataattgccc cggatgcccc aacgctgatg actatctgct cactttcgcg
     8941 taacgcataa caggcgctga tcagcccacc aaagatgccg gagaataacc agataatcag
     9001 gagtcgccat ttccccagta tccgttccgc cactgtcccc accaccagga gtgcaaacat
     9061 attcatcagc aaatgggaaa aactactgtg cagaaaaaca ctgctcaata agcgccagga
     9121 ttcatccgac aacgtgagag aagcaacgtt agcgccgaga tggatgagtg cggattgatc
     9181 gacttcttca taactcatcc attgtgcgag gtaacaaaat atcccaacac agagcgcaaa
     9241 aaggatcagg gttattgcgg gtaattgaac atttaacggc ttaaccgaag atgcagacat
     9301 aaggtgattc ttccttgaaa agactagtaa aaagcatttt cccgagtgta gttatcatcc
     9361 cggcaaacgt cccacaccga aaagttaaca ctgtctgaaa catgactttt tcaaactgaa
     9421 attacaatcg taattaattc acccctaagt attttttggc atgtaagttg ctttatttac
     9481 tgtgtaagca atgaaatcag ccgcaacgca accgggttgc accaccacaa atcagcggta
     9541 gcaccaaaaa ggtgcggttt aagaattttg cttcggaatg gtgcggcgca cctcgcaaaa
     9601 ataaacaata aaaaagcaat atctttacat ttatccaaac taaatgttac ctaacaaaca
     9661 caacactgca caataaagtc gcagatgata acaacacaaa cactcacaac gggtatccat
     9721 gcgtttttaa cgcagaagat aaaggagttg gatatgcaat tacgtaaacc tgccacagca
     9781 atcctggccc tggcgctttc cgcaggactg gcacaggcag atgacgccgc cccggcagca
     9841 ggcagcacgc tggacaaaat cgccaaaaac ggcgtgattg tcgtcggtca ccgtgaatct
     9901 tcagtacctt tctcttatta cgacaatcag caaaaagtgg tgggctactc gcaggattac
     9961 tccaacgcca ttgttgaagc agtgaaaaag aaactcaaca aaccagactt acaggtgaaa
    10021 ttgattccga tcacctcaca aaaccgtatt ccactgctgc aaaacggcac tttcgatttt
    10081 gaatgcggtt ctaccaccaa caatgccgaa cgccaaaaac aggcggcttt ctctaacact
    10141 attttcgtgg tcggtacgcg cctgctgacc aaaaagggcg gtgatatcaa agattttgcc
    10201 gacctgaaag gcaaagccgt agtcgtcact tccggcacca cctctgaagt tttgctcaac
    10261 aaactgaatg aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac
    10321 tctttccgta ccctggaaag cggtcgtgcc gttgccttta tgatggatga tgctctgctg
    10381 gccggtgaac gtgcgaaagc gaagaaacca gacaactggg aaatcgtcgg caagccgcag
    10441 tctcaggaag cctatggttg catgttgcgt aaagacgatc cgcagttcaa aaagctgatg
    10501 gatgatacca tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataagtgg
    10561 ttcaaaaatc caattccgcc gaaaaacctg aacatgaatt tcgaactgtc agacgaaatg
    10621 aaagcactgt tcaaagaacc gaatgacaag gcactgaact aattacaaga accaggggcg
    10681 gaaattccag ccctctcgat tgttacgtag cacggacaga ctatacgcct ggtggtcgtt
    10741 ccccatcggg ccagaaaacc gcaatacgct gggtaacaat cttcgagggt agcagttaac
    10801 gctgctaccc tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt
    10861 tttacaacaa gccccgttcg gcaacaccac ctatcttggt tggatctgga gtggctttca
    10921 ggtgacgatc gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt
    10981 cggcatttta cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga
    11041 actgttccgc aacgtgccgt tgattgtgca attctttacc tggtatctgg tgatcccgga
    11101 gctgctgcca gagaaaatcg gcatgtggtt taaggcggaa ctggacccta atattcaatt
    11161 tttcctttca tccatgctct gcctggggct gtttaccgcc gcccgtgttt gcgaacaggt
    11221 acgcgccgct attcagtcgc tgccgcgtgg gcaaaaaaat gccgcgctgg cgatgggtct
    11281 gacgctaccg caggcctacc gctacgtact gctgcctaat gcttatcgcg ttatcgtccc
    11341 gccgatgacc tcagagatga tgaacctggt gaaaaactcc gccatcgcct cgactatcgg
    11401 tctggtggat atggcggcgc aggcgggtaa attgctcgat tactcggccc acgcctggga
    11461 gtcgtttacc gccattacgc tggcgtatgt attgattaac gcttttatca tgctggtgat
    11521 gacgctggtt gaacgtaaag ttcgcctgcc cggcaacatg gggggcaaat aatgtacgag
    11581 tttgactgga gttccattgt tccttccctg ccatatctgc tcgacgggct ggtgatcacg
    11641 ctgaaaatca ccgtcacggc agtggtgatt ggtattttgt ggggcaccat gctggcggtg
    11701 atgcgcttgt ccagctttgc gcccgtagcg tggtttgcca aagcctacgt taacgtattc
    11761 cgctcgattc ctttagttat ggttttgctg tggttttacc tgatcgtacc gggttttctg
    11821 caaaacgtgc tgggattatc acccaaaaat gatatccgtt taatttcggc aatggtggcg
    11881 ttttcgatgt ttgaagcggc ctactattca gagattatcc gtgctggtat tcaaagtatt
    11941 tctcgtggtc agtcgagcgc cgcgctggcg ttgggaatga ctcactggca gtcgatgaaa
    12001 ctgattattc tgccgcaggc gttccgcgcg atggtgccgc tgttgctcac tcagggcatc
    12061 gtactgtttc aggatacctc actggtgtat gtgttatctc tggccgattt cttccgtacc
    12121 gcctcaacca ttggtgagcg tgatggtacg caggttgaga tgatcctgtt tgccggattt
    12181 gtttatttcg ttattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca
    12241 gcataatgat taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg
    12301 actgctcaac cgaagtgaaa aaaggcgaag ttgtggtggt ttgcggcccg tctggttccg
    12361 gcaaatcaac gctgattaaa accgtcaacg gcctcgaacc ggtgcagcaa ggtgaaatca
    12421 ccgtcgatgg catcgtggtt aacgacaaga aaaccgatct ggcgaaactg cgttcccgcg
    12481 tcgggatggt gtttcagcat ttcgagctgt tcccccatct gtcgattatc gaaaacctga
    12541 ccctggcgca ggtgaaagtg cttaaacgca ataaagcgcc ggctcgtgaa aaagcattga
    12601 aattgctgga gcgcgtcggg ctttccgcac atgccaataa gtttccggcg cagctttccg
    12661 gcggtcagca gcagcgtgtg gcgatcgctc gcgcattgtg catggatcct attgccatgc
    12721 tgttcgacga accaacgtcg gcgctggatc cggagatgat caacgaagtg ctggacgtga
    12781 tggtcgaact ggcgaacgaa ggaatgacga tgatggtggt gacccacgaa atgggctttg
    12841 cccgtaaagt ggcgaatcgg gtgatcttta tggacgaggg taaaattatc gaagactcgc
    12901 cgaaaggcgc tttcttcgat aatccaaaat cggaccgcgc aaaagacttc ctcgcaaaaa
    12961 tcctgcatta atcactctgg cgcgcatact caattgcgcg ccatctcacg ctttctcgtt
    13021 tcgtccctcg tatctgttac atcccggcgt tacccttgtc gcaaagaaac acacaacaag
    13081 gagcaacaat ggcactgcca attctgttag attgcgaccc aggtcatgac gacgctatcg
    13141 caatcgttct cgccctcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg
    13201 gaaaccagac accagaaaaa accttacgca atgttctgcg tatgctgacc ttgcttaatc
    13261 gcaccgatat tccggtagca agcggtgcgg taaaaccgtt aatgcgtaat ttgattattg
    13321 cggacaacgt gcacggcgaa agcggtctcg acggcccggc attaccggaa ccgacgttcg
    13381 caccgcaaaa ctgtacggcg gtggagctga tggcgaaaac gctgtgtgaa agtgaggaac
    13441 ctgtcaccat tgtttctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccacc
    13501 cggaactgca tagcaaaatt gcccgtatcg tgattatggg cggcgcaatg gggcttggta
    13561 actggacgcc tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct
    13621 tccagtcagg aattccggtg gtgatggccg gtctggatgt tactcacaaa gcacaaatcc
    13681 acgttgaaga cactgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg
    13741 aactgctgga tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac
    13801 cactgcatga cccatgcacc atcgcctggc tgttgaaacc ggagttgttt accactgttg
    13861 agcgctgggt tggcgtggaa acacaaggga aatataccca gggtatgaca gttgttgatt
    13921 attattatct gactggcaat aaaccgaatg ccaccgtaat ggtcgatgtt gatcgtcagg
    13981 gctttgttga tttactggct gatcgtctga aattttacgc ttaatcactc gccggatgac
    14041 atcacgccat ccggcaccac tcatttcatt acggctcgaa cggacgtctc tggaactgat
    14101 catgaccaca tttcgggcat agcgtcagca cttccggtgt atagatcggg agatggaagt
    14161 gacatttctc gcagaccaga tttcccagcc ctaccacttc tccgctgtga taaaccccgt
    14221 gatgattgag gtcctggaaa acttctcgcc attcaagctg cgttttatcg gtgatgtctg
    14281 ccagctcctg ccacaagctt tctttaatca cccgcataaa gacgctgtca gactcttctt
    14341 tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtctgaca gctcgcgtca
    14401 gctcatcgac ctcggttcgc gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt
    14461 ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt gacgcgacca
    14521 gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg ttaactgact
    14581 ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact ctgtaaatta
    14641 atgtatgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg
    14701 ggcgtttggg ctatgctatg cggatctgaa aaaccacatc aacgctacat ttgtagccgt
    14761 attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag agatagaatc
    14821 caaagtacag cttcactggg atgagaagcg cacatttgaa gtaaccgaag acgagagcaa
    14881 agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac acatgggcca
    14941 cgttcgcaac tacaccatcg gtgacgtgat cgcccgctac cagcgtatgc tgggcaaaaa
    15001 cgtcctgcag ccaatcggct gggacgcatt tggtctgcct gcggaaggcg cggcggtgaa
    15061 aaacaacacc gcgccagcac cgtggacgta cgacaacatc gcgtatatga aaaaccagct
    15121 caaaatgctg ggctttggtt atgactggag ccgcgagctg gcgacctgta cgccggaata
    15181 ctaccgttgg gaacagaaat tcttcaccga actgtataaa aaaggcctgg tatataagaa
    15241 gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg aacaagttat
    15301 cgacggctgc tgctggcgct gcgataccaa agttgagcgt aaagagatcc cgcagtggtt
    15361 tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac tggatcactg
    15421 gccagacacc gttaaaacaa tgcagcgtaa ctggatcggt cgttccgaag gcgtggaaat
    15481 caccttcaac gttaaggact atgacaacac gctgaccgtt tacactaccc gcccggacac
    15541 ctttatgggt tgtacctacc tggcggtggc tgcgggtcat ccgctggcac agaaagcggc
    15601 ggaaaataat cctgaactgg ccgcctttat tgacgaatgc cgtaacacca aagttgccga
    15661 agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag cggttcaccc
    15721 attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg aatacggcac
    15781 gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg cctctaaata
    15841 cggcctgaac atcaaaccgg ttatcctggc agctgacggc tctgagccag atctttctca
    15901 gcaagccctg actgaaaaag gcgtgctgtt caactctggc gagtttaatg gtcttgacca
    15961 tgaagcggcc ttcaacgcca ttgccgacaa actgactgaa atgggcgttg gcgagcgtaa
    16021 agtgaactac cgcctgcgcg actggggtgt ttctcgtcag cgttactggg gcgcgccgat
    16081 tccgatggtg acgctggaag acggtaccgt aatgccgacc ccggacgacc agctgccggt
    16141 gatcctgccg gaagatgtgg tgatggacgg aattaccagc ccgattaaag cagatccaga
    16201 gtgggcaaaa actaccgtta acggtatgcc agcactgcgt gaaaccgaca ctttcgacac
    16261 ctttatggag tcctcctggt actatgcgcg ctacacttgc ccggagtaca aagaaggtat
    16321 gctggattcc aaagcggcta actattggct gccggtggat atttacattg gtggtatcga
    16381 acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc gtgatgcagg
    16441 catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga
    16501 tgccttctac tatgttggtg aaaacggcga acgtaactgg gtttccccgg ttgatgctat
    16561 cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact
    16621 ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt
    16681 gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg cttctccggc
    16741 tgatatgact ctcgaatggc aggaatccgg cgtggaaggg gctaaccgct tcctgaaacg
    16801 tgtctggaaa ctggtgtacg agcacacagc aaaaggtgat gttgcggcac tgaacgtcga
    16861 tgtgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacta tcgctaaagt
    16921 aaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga ttatggagct
    16981 gatgaacaaa ctggcgaaag caccgaccga tggcgagcag gatcgcgccc tgatgcagga
    17041 agcgctgctg gcggtagtcc gtatgcttaa cccgttcacc ccgcacatct gcttcacgct
    17101 gtggcaggaa ctgaaaggcg aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga
    17161 aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta aagtccgtgc
    17221 aaaaatcacc gttccggtgg acgcaacgga agaacaggtt cgcgaacgtg ctggccagga
    17281 acatctggta gcaaaatatc ttgatggcgt tactgtacgt aaagtgattt acgtaccagg
    17341 taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca
    17401 ttgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct gcgtgacacc
    17461 acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc gaacgggcca
    17521 ttaagccgtg cggtgcgtaa tcagttacgt ctgaatggtg tcgagttgct tgataaagaa
    17581 accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc gaaagatacc
    17641 gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac ggtgaatgcg
    17701 accgtgttga tccccggccg tgatatctac ccgattagcg ccaaagtctt ccgttcgttc
    17761 ttcgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat cgtaaaagag
    17821 atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg tgctgcggat
    17881 attcgttccg acgaagaaca gacgtcgacc acaacggata ctctggcaac gcctgcacgc
    17941 gtctccacca cgctgggtaa ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc
    18001 tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg ttattgcagg
    18061 aaagccagga cgctgttcgt caggtagctg cggcacaagg attcgaagaa caccacactt
    18121 tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag gctatgagtc
    18181 tgtttgccag tagacaaacg ctattgctgt tgttacccga aaacggaccg aatgcggcga
    18241 tcaatgaaca acttctcaca ctcaccggac ttctccatga cgacctgctg ttgatcgtcc
    18301 gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgct cttgcgaatc
    18361 gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc tgggttgctg
    18421 cgcgcgcaaa acagctcaac ttagaactgg atgacgctgc aaatcaggtg ctctgctact
    18481 gttatgaagg taacctgctg gcgctggccc aggcactgga gcgtttatcg ctactctggc
    18541 cggacggcaa actgacatta ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca
    18601 ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgcg ttgcatattc
    18661 ttcagcagct gcgtctggaa ggcagcgaac cggttatttt gttgcgcaca ttacaacgtg
    18721 aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt
    18781 ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gagcgaggcg ttaaatcgct
    18841 taagccagcc gcagttacgt caggccgtgc aactcctgac acgaacggaa ctcaccctca
    18901 aacaagatta cggtcagtca gtgtgggcag agctggaagg gttatctctt ctgttgtgcc
    18961 ataaacccct ggcggacgta tttatcgacg gttgatatga aatctttaca ggctctgttt
    19021 ggcggcacct ttgatccggt gcactatggt catcttaaac ccgtggaaac gctggcgaat
    19081 ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca tcgtccccag
    19141 ccggaagcga acagcgcgca gcgtaaacac atgcttgaac tggcgatagc tgacaagcca
    19201 ctgtttaccc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca
    19261 ctgaaagagt ggcggcagga gcaaggaccg gacgtgccgc tggcgtttat tattggtcag
    19321 gattcattgc tgacctttcc gacctggtac gaatacgaaa cgatactcga caatgcacat
    19381 ttgatcgtct gtcggcgtcc tggttaccca cttgaaatgg cgcaaccgca ataccagcaa
    19441 tggctggagg atcatttgac acataacccg gaagatcttc accttcagcc tgccggtaaa
    19501 atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg cgaacgtttg
    19561 caaaatggtg aatcgtgtga ggatttatta ccggaaccgg tactgactta cattaaccaa
    19621 caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg tggttaattc
    19681 gtcatggtga aacgcaagcg aatgtcgatg gtctgtacag cggtcatgcg cccacacccc
    19741 tgaccgcgcg cggtattgag caagcgcaaa atctgcatac gctgctacat gatgtttcct
    19801 ttgacctggt tttatgcagt gaactggaac gggcacagca taccgcgcga ctggttctta
    19861 gtgaccgcca gcaccccgtt catatcatcc ctgaactcaa tgaaatgttt tttggcgact
    19921 gggagatgcg acatcatcgt gacctcatgc aagaagatgc cgaaaactat agcgcgtggt
    19981 gcaatgactg gcagcatgct atccccacga acggtgaagg atttcaggca ttttcgcaac
    20041 gtgtggaacg atttatcgca aggcttagtg aatatcagca ctatcagaat attttagtcg
    20101 tcagccatca gggcgtactg agtctgttaa ttgctcgttt aattggcatg cctgccgagt
    20161 ctatgtggca tttccgcgtt gaccaggggt gttggagcgc cattgatatc aaccaaaaat
    20221 tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaatgca tgacttttct
    20281 gtttttttac gggtaagccg caacgaccat tgacagcccc gggcaggctg atattctccg
    20341 cagccagact ttttccgcca gacacgactt tgtagaaatt gttttacaaa aatggcgatg
    20401 caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg gcgacaattg
    20461 tccctaaatc gcctctggtt caggtatact gacagaccat ttttatctat ttgattcacc
    20521 cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa aattgatgac
    20581 ctcaaaggtc aggacatcat cgccttagac gttcagggca aatccagcat caccgactgc
    20641 atgatcatct gtacgggtac gtccagccgc catgttatgt ccattgctga tcacgttgtg
    20701 caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac
    20761 tggattgtcg tggatttggg tgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc
    20821 ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga
    20881 acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa
    20941 gatatgccct tcgagctgat tgaaattccg gccggaaaac gcggcaagaa tgcggacatc
    21001 aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt
    21061 gtcaccctcg atattccagg caagccctgg gatacgccgc agttagccgc tgagctggaa
    21121 cgctggaagc tggatggtcg cgacgtcagt ctacttattg gcgggcctga agggttgtcg
    21181 cctgcctgta aagcggcggc ggagcagagc tggtcgctgt cggcacttac ccttccccat
    21241 ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat caccaccaac
    21301 catccttatc accgtgagtg ataagtgagc tttgagtaga aaacgcagcg gatgaaacta
    21361 cagaactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc
    21421 gcctttttgg ggattttgct gctaaccggc gtgcttatcg ccaacctgta taatctgcaa
    21481 attgttcgct ttaccgacta ccagacccgc tctaatgaaa accgcattaa gctggtgcct
    21541 atcgcaccca gccgcggcat tatctacgac cgtaacggta tccctctggc tctcaaccgc
    21601 actatctacc agatagaaat gatgccggag aaagtcgata acgtgcagca aacgctggac
    21661 gctttgcgca gcgtggttga tctgaccgat gatgatattg ctgcattccg aaaagagcgc
    21721 gcacgttcac accgtttcac ctctattccg gtgaaaacta acctgaccga agtacaagta
    21781 gctcgctttg ccgtcaacca gtaccgtttt ccgggtgttg aagttaaagg ctataaacgt
    21841 cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac
    21901 gataaagacg ttgaacgcct gaataatgac ggcaaactgg ccaactatgc ggcaacgcat
    21961 gatatcggta agctgggcat tgagcgttac tatgaagacg tgctgcacgg tcagaccggt
    22021 tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa agaagtacca
    22081 ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca atatattgaa
    22141 acgctgctgg cgggtagccg cgcagctgtg gtagtcaccg atccgcgtac cggtggggtg
    22201 ttggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg tatctccagc
    22261 aaagattatt ccgccttgct gaacgacccg aatacaccgc tggtgaaccg cgccacacag
    22321 ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc attgagtgcc
    22381 ggggtgatca cgcgcaatac gacgctgttt gaccccggct ggtggcaact gccagggtcg
    22441 gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg
    22501 ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg gatcgatcgc
    22561 ctctccgaat ggatggggaa attcggttat ggtcattaca ccggtatcga cctggcggaa
    22621 gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg
    22681 tatcagggtg acaccattcc ggttggtatc ggtcagggtt actggacggc gacccctatc
    22741 cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt tccgcatttg
    22801 ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacaaccgca tgaaccgccc
    22861 gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta cggtgttgct
    22921 aaccgcccta acggtacggc gcataaatat tttgccagcg caccgtacaa aattgcggcg
    22981 aaatccggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa
    23041 attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata caacaatccg
    23101 caagtggctg tcgccatgat tctggagaac ggtggcgcgg gtccggcggt tggtacgctg
    23161 atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct gcctgcggaa
    23221 aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg aataaaaaaa
    23281 cattctggga taaagtccat ctcgatccca caatgctgct gatcttactg gcattgctgg
    23341 tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg atggagcgta
    23401 aaatcggcca gattgcgatg ggtctggtca tcatggtggt gatggcgcaa attcctccac
    23461 gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg ctggtggcgg
    23521 tagatgcttt cggtgccatc tctaaaggcg ctcaacgctg gctggacctc ggtattgttc
    23581 gttttcaacc gtcggaaatt gccaagatag ccgtaccgtt gatggttgca cgctttatca
    23641 accgcgacgt ttgcccacct tcattgaaga atacagctat cgcgctggtg ctgatattta
    23701 tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc gttgcgcttt
    23761 ccggcctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc gtcgcagtag
    23821 tgctggtagc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat taccagcgcc
    23881 agcgcgtaat gatgctcctg gacccggaat cagacccact cggcgcaggc tatcacatta
    23941 ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg ctgcacggca
    24001 ctcagtcaca gcttgaattt ctccccgaac gccataccga ctttatcttc gcggtactgg
    24061 cggaagagct gggattagtg ggcattttga ttctgctcgc tctttacatt ctgctgatca
    24121 tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc atggctggcg
    24181 gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta agcggtattc
    24241 tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg ctaattgtgc
    24301 tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa
    24361 gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg
    24421 ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc gcagcctgcg
    24481 gtatgtaacg gccccatagt tgaaattagc ggggcagacc cgcgtttcga accactgaac
    24541 gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg
    24601 tctcgattta gccaggcggg actggcggca atctatgatg ccgaacccgg cagtaacctg
    24661 acggcctctg gcgaagcttt cgatccgaca cagttgacgg cggcccatcc aacgcttcca
    24721 atccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt
    24781 aatgatcgcg gcccttatgg caacgaccgc gtgatttcgc tttctcgcgc ggcagctgac
    24841 cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt cgcccaggat
    24901 ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac ttacgccctg
    24961 ccagcacctc ccgatttaag cggtggcgcg ggaacaagtt cagtgcctgt cccgcagggt
    25021 gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag atccgaccgg cgcgccggta
    25081 accagcagcg gtttcctcgg cgcaccaacg accttagcgc ctggtgtact ggaaggcagc
    25141 gaaccgacgc ctgcgccaca gcccgttgtt acagctccgt cgacaacgcc tgcaacctca
    25201 cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt tatggtgcaa
    25261 gtcggggccg ttagcgatca ggctcgtgcg caacagtacc aacagcaact gggacagaag
    25321 ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcggatcca gcttggccca
    25381 ttcgccagca aagccgaagc cagtaccttg cagcaacgtt tgcaaaccga agcccaatta
    25441 cagtcattta ttaccaccgc gcagtagcgt aaagcaggca tctgatgtgt caatttctgt
    25501 aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc atttgctata
    25561 gtagggcact ttttttaatt ccatcacgga tgtcgtagtt cagaccatga ataccatttt
    25621 ttccgctcgt atcatgaagc gcctggcgct caccacggct ctttgcacag cctttatctc
    25681 tgctgcacat gccgatgacc tgaatatcaa aaccatgatc ccgggtgtac cgcagatcga
    25741 cgcggagtcc tacatcctga ttgactataa ctccggcaaa gtactcgccg aacagaacgc
    25801 tgatgtccgc cgcgatcctg ccagcctgac caaaatgatg accagttacg ttatcggcca
    25861 ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc actatcggca acgacgcatg
    25921 ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac cgggcatgca
    25981 ggttccggtt tctcagctga tccgcggtat taacctgcaa tcgggtaacg atgcttgtgt
    26041 cgccatggcc gattttgccg ctggtagcca ggacgctttt gttggcttga tgaacagcta
    26101 cgttaacgcc ctgggcctga aaaataccca cttccagacg gtacacggtc tggatgctga
    26161 tggtcagtac agctccgcgc gagatatggc gctgatcggc caggcgttga tccgtgacgt
    26221 accgaatgaa tactcgatct ataaagaaaa agaatttacg tttaacggta ttcgccagct
    26281 gaaccgtaac ggcctgttat gggataacag cctgaatgtc gacggcatca aaaccggaca
    26341 cactgacaaa gcaggttaca accttgttgc ttctgcgact gaaggccaga tgcgcttgat
    26401 ctctgcggtg atgggcggac gtacttttaa aggccgtgaa gccgaaagta aaaaattgct
    26461 aacctggggc ttccgtttct tcgaaaccgt taacccactg aaagtaggta aagagttcgc
    26521 ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg ttaggggttg ataaagacgt
    26581 gtacctgacc attccgcgtg gccgcatgaa agatctgaaa gccagctatg tgctgaacag
    26641 cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc ggtactatca acttccagct
    26701 tgatggcaaa acgatcgaac aacgcccgct ggttgtgctg caagaaatcc cggaaggcaa
    26761 cttcttcggc aaaatcattg attacattaa attaatgttc catcactggt ttggttaaaa
    26821 atcgaacact tgaaagtgta atttccgtcc ccatatacta agcatcagta aaaaactccc
    26881 gccttctggc gggagttgct atttaattac gttacgccgg agctgacatg aaaaccaaac
    26941 ttaacgaact gcttgaattc cctactcctt ttacttacaa agttatgggg caggcgttac
    27001 ctgagctggt tgatcaggtg gttgaagtgg tacagcgcca tgcgccaggt gactacaccc
    27061 caacggtaaa accaagcagc aaaggcaact accactcggt atctatcact atcaacgcga
    27121 ctcatatcga gcaggttgaa acactgtatg aagaactggg caaaatcgat attgtccgca
    27181 tggttctgta actcgcttct ccgttacccg gctcctggtc gggtaactcc cctctcctcg
    27241 ctgtgatata cttttccccc acttttactc attctccacg gagatgccgt tttgtatcag
    27301 gataaaattc ttgtccgcca gctcggtctt cagccttacg agccaatctc ccaggctatg
    27361 catgaattca ccgatacccg cgatgatagt acccttgatg aaatctggct ggtcgagcac
    27421 tatccggtat tcacccaagg gcaggcagga aaggcggagc atattttaat gccgggtgat
    27481 attccggtga tccagagcga tcgcggtggg caggtgactt atcacgggcc ggggcaacag
    27541 gtgatgtatg tgttgcttaa cctgaaacgc cgtaaactcg gtgtgcgtga actggtgacc
    27601 ttgcttgagc aaacagtggt gaataccctg gctgaactgg gtatagaagc gcatcctcgg
    27661 gctgacgcgc ctggtgtcta tgtcggggaa aagaaaattt gctcactggg tttacgtatt
    27721 cgacgcggtt gttcattcca cggtctggca ttaaacgtca atatggatct ttcaccattt
    27781 ttacgtatta atccttgtgg gtatgcagga atggaaatgg ctaaaatatc acaatggaaa
    27841 cccgaagcga cgactaataa tattgcacca cgtttactgg aaaatatttt agcgctacta
    27901 aacaatcctg acttcgaata tattaccgct taattccata catcaatggc ccaatttaca
    27961 ctgggtcatt actcataata cctgctaatg ctttacatct ccttcgcgtt tgtatatttt
    28021 ctttatcact tgcagaaatg cgatgctacg ccttgctccg tagctcaccc aattggttaa
    28081 ctcattgaaa taattacc
//
LOCUS       DADTFG010000059        28074 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.059, whole genome shotgun sequence.
ACCESSION   DADTFG010000059 DADTFG010000000
VERSION     DADTFG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.059"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(10..1464)
                     /gene="xylB"
                     /locus_tag="J1138_002261"
     CDS             complement(10..1464)
                     /gene="xylB"
                     /locus_tag="J1138_002261"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBA8454055.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQCYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1536..2858)
                     /gene="xylA"
                     /locus_tag="J1138_002262"
     CDS             complement(1536..2858)
                     /gene="xylA"
                     /locus_tag="J1138_002262"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBA8454056.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3224..4216
                     /gene="xylF"
                     /locus_tag="J1138_002263"
     CDS             3224..4216
                     /gene="xylF"
                     /locus_tag="J1138_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8454057.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4294..5835
                     /gene="xylG"
                     /locus_tag="J1138_002264"
     CDS             4294..5835
                     /gene="xylG"
                     /locus_tag="J1138_002264"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBA8454058.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGSISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5813..6994
                     /gene="xylH"
                     /locus_tag="J1138_002265"
     CDS             5813..6994
                     /gene="xylH"
                     /locus_tag="J1138_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBA8454059.1"
                     /translation="MSKSNPSEVKLAVPTSGGLSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7072..8250
                     /locus_tag="J1138_002266"
     CDS             7072..8250
                     /locus_tag="J1138_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HBA8454060.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8358..9182)
                     /locus_tag="J1138_002267"
     CDS             complement(8358..9182)
                     /locus_tag="J1138_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBA8454061.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9502..11532
                     /gene="malS"
                     /locus_tag="J1138_002268"
     CDS             9502..11532
                     /gene="malS"
                     /locus_tag="J1138_002268"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBA8454062.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISLQNTVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTSASGLPVFYKNKTDTHAKDIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHDFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11710..12963
                     /gene="avtA"
                     /locus_tag="J1138_002269"
     CDS             11710..12963
                     /gene="avtA"
                     /locus_tag="J1138_002269"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBA8454063.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13115..13588)
                     /locus_tag="J1138_002270"
     CDS             complement(13115..13588)
                     /locus_tag="J1138_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA8454064.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13690..14538)
                     /gene="yiaJ"
                     /locus_tag="J1138_002271"
     CDS             complement(13690..14538)
                     /gene="yiaJ"
                     /locus_tag="J1138_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBA8454065.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14739..15736
                     /gene="yiaK"
                     /locus_tag="J1138_002272"
                     /pseudo
     CDS             14739..15736
                     /gene="yiaK"
                     /locus_tag="J1138_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
     gene            15748..16212
                     /locus_tag="J1138_002273"
     CDS             15748..16212
                     /locus_tag="J1138_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA8454066.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            16235..17155
                     /locus_tag="J1138_002274"
     CDS             16235..17155
                     /locus_tag="J1138_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HBA8454067.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINALQANKVLALELQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
     gene            17269..17736
                     /gene="yiaM"
                     /locus_tag="J1138_002275"
     CDS             17269..17736
                     /gene="yiaM"
                     /locus_tag="J1138_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HBA8454068.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEK
                     TAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            17739..19016
                     /gene="yiaN"
                     /locus_tag="J1138_002276"
     CDS             17739..19016
                     /gene="yiaN"
                     /locus_tag="J1138_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBA8454069.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGELMNAGGLSQRIVDLPMKLVGHRLGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLLTIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            19029..20015
                     /gene="yiaO"
                     /locus_tag="J1138_002277"
     CDS             19029..20015
                     /gene="yiaO"
                     /locus_tag="J1138_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HBA8454070.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            20019..21515
                     /gene="lyxK"
                     /locus_tag="J1138_002278"
     CDS             20019..21515
                     /gene="lyxK"
                     /locus_tag="J1138_002278"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HBA8454071.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            21512..22174
                     /gene="ulaD"
                     /locus_tag="J1138_002279"
     CDS             21512..22174
                     /gene="ulaD"
                     /locus_tag="J1138_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBA8454072.1"
                     /translation="MSRPLLQLALDHSSIEAAQRDVTRLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRSRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRALAGAANPAQVAGDFHAQIDSIWGGK
                     RA"
     gene            22167..23027
                     /locus_tag="J1138_002280"
     CDS             22167..23027
                     /locus_tag="J1138_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBA8454073.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            23021..23716
                     /gene="araD"
                     /locus_tag="J1138_002281"
     CDS             23021..23716
                     /gene="araD"
                     /locus_tag="J1138_002281"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA8454074.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(23763..25301)
                     /locus_tag="J1138_002282"
     CDS             complement(23763..25301)
                     /locus_tag="J1138_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBA8454075.1"
                     /translation="MTNNPPSARVQPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEIDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(25409..26704)
                     /locus_tag="J1138_002283"
     CDS             complement(25409..26704)
                     /locus_tag="J1138_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBA8454076.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLIRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(26834..27985)
                     /gene="yiaY"
                     /locus_tag="J1138_002284"
     CDS             complement(26834..27985)
                     /gene="yiaY"
                     /locus_tag="J1138_002284"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBA8454077.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         6928 a         7217 c         7059 g         6870 t
ORIGIN      
        1 ggataacgtt tacgccatta atggcagaag ttgctgatag aggcgacgga acgtttctcg
       61 tcgtggctga taagcggcat aacactgcgc atctggtaga tgcgactgtt ctaacggtag
      121 ttgcggcaac aactcaatga gcgatttctc tggattcgcc gcaatctgcg ccagccttgc
      181 tgcgcccagt gctggcccca catcgccacc cgtgcggtaa tccagttgct gaccgctgat
      241 gtccgccagc atttgacgcc agtactcact acgcgccccg ccaccaatca acgtaacact
      301 ttgcggttta atgccgcagg catgcacgac atccatgcca tctgccagcg cataacccac
      361 gccttccagc actgctcgcg ccagttcatt ggggccatgt tgatgagtca aaccaaagaa
      421 aacccctttc gcctggggat tattgtgtgg tgtacgctcg ccggaaagat aaggcagaaa
      481 ccaaactggc tcggcacttt catcagcctg tcgagctgca gtgattaaag cagggacatt
      541 gctcaggccg gttaatttcg cggcccaatc cagacacgaa gccgcactca gcattacaga
      601 cattaaatgc caacgttgcg gtaacgcatg gcaaaagcta tgtacggcgc tttctggctt
      661 gcttaagaac ccttcgctga cagcaaaata gacccccgac gtccccagcg ataacattgc
      721 ctgattagca tcaaccattc ccacaccaac cgcaccagct gcattgtcgc caccgcctgc
      781 gataactggc accgccgcca taccccacgc tttcgcaact tcaggtaaca aagcaccagt
      841 aatttcgctg ccttcgtata atgcgggcat ctggtcacga gataagtggc aagcctgcag
      901 catgagatcg ctccagtcac gctttgcgac atccagccac atagtgccag ctgcgtcaga
      961 catatcgctg gcaaactccc ccgtcatacg cagacgcaag taatctttcg gtaataatac
     1021 tttatcgatt tgacggaata tctccggctc atgccgctga acccatagca atttaggcgc
     1081 agtaaatccg ggcatcatca ggttgccggt aatcacccgc gattgcggaa ctcgcgcttc
     1141 cagcaaagtg cactcttgcg cacagcgccc gtcgttccac aaaatggcag ggcgtaacac
     1201 ccgttgctga gcatccagta aggttgcacc gtgcatctgt ccggcaatac ctaatgcttt
     1261 aacgtcttgc agagaatgct gaacgcccag agctttcatt gcgcgatcag ttgcctgcca
     1321 ccactgttcc gggtcttgtt ccgaccagag tggatgcggg cgcgaaacgg tcagcttttc
     1381 cgtttgcgca gcaaccacct caccctgctc gttgagcaaa ataactttta cgcccgaggt
     1441 gccaagatct atcccgatat acatatcgat cgttccttaa aaaaatgccc ggtatcgcta
     1501 ctgataaccg ggccaacgga ctgcacagtt agccgttatt tgtcgaacag ataatgattt
     1561 accagatttt ccagttgctc ctggcgacca ctctgatgca ccggagacaa attatgttcc
     1621 tgagcatatt tggctaaatc tgccagtgac atttggcctt tcaggatttg ctggcctaat
     1681 tcactattcc agccggaata acgctgcgcg atgcgtttat ccagctcgcc atcttcaatc
     1741 atgcgcgctg caattttcag cgccaacgcc atcgtatcca tcgcgccgat atgaccgtaa
     1801 aacagatcat atttatcagt actttgacga cgtactttgg catcgaagtt cagaccaccg
     1861 gtggtgaaac cgcctgcttt gagaatttca tacatcacca gcgcattctc ttccacactg
     1921 ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc cacggttggc gtcgacagaa
     1981 ccgaacaggc caagcgcaat ggcggtggct atttcatgat ggaaagagtg acctgccagc
     2041 gtcgcgtggt tagcttcaat attcagttta atctcttttt ccagaccaaa ctgtttcagg
     2101 aagccataga ccgtcgcagc atcgtaatca tattgatgtt tggtcggttc ttgcggtttc
     2161 ggttcgataa gcaacgtgcc ctggaaaccg attttatgtt tatgctcaac caccatctgc
     2221 ataaagcggc ccagctgttc acgctcctgg cgcaagtcgg tatttaacag cgtttcgtaa
     2281 ccttcacgac caccccacag gacatagttt tcaccgccca atttatgggt tgcttccatc
     2341 gctgtaacaa cttgcgttgc cgcccaactg aagacttcag gatctgggtt cgtcgccgca
     2401 cccgcgccat agcgagggtt tgtaaagcag ttagcagttc cccacagcag cttcacgccg
     2461 ctctcttctt gcttgcctgc caggacatca accatttgcg caaaattatt gatgtactct
     2521 tttaacgacg cgccctcagg ggaaacatcc acatcgtgga agcaataaaa tggcacatgt
     2581 aacttgtgga aaaactcaaa tgcgacatct gctttacgct tcgccaacgc cagtgcctca
     2641 ccaggctgct gccacggacg attaaacgct cccacaccaa acatatccgc cccgttccag
     2701 cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct cttccatacg cttgcccaac
     2761 accagttcgt cgggattgta gtgacggaat gctaacgggt ttgaggattt tgagccttca
     2821 taacgaacgc gatcgagctg gtcaaaatag gcttgcatat tgaactccat aatcaggtaa
     2881 tgccgcgggt gatggatgat gtcgtaatat tgggcactcc ctttcagttg ctcaattatg
     2941 ttatttcaca ctgctattga gataattcac aagtgtgcgc tcgctcgcaa aataaaatgg
     3001 gatgatgaaa ctgggtaatt cctcgaagag aaaaatgcaa taagtacaat tgcgcaacaa
     3061 aagtaagatc tcggtcataa atcaagaaat aaaccaaaaa tcgtaatcga aagataaaaa
     3121 tctgtaattg ttttcccctg tttagttgct aaaaattggt tacgtttatc gcggtgattg
     3181 ttacttatta aagctgtcct ctaactacag aaggccctac accatgaaaa taaagaacat
     3241 tctactcacc ctttgcacct cacttctgct taccaacgtt gcggcacacg ccaaagaagt
     3301 caaaataggt atggcgattg atgatctccg tcttgaacgc tggcaaaaag atcgagatat
     3361 ttttgtgaaa aaagcagaat ctctcggcgc gaaagtattt gtacagtctg caaatggcaa
     3421 tgaagaaaca caaatgtcgc agattgaaaa catgattaac cggggtgtcg atgttcttgt
     3481 cattattcct tataacggtc aggtattaag taacgttgta aaagaagcca aacaagaagg
     3541 tattaaagta ttagcttacg accgtatgat taacgatgcg gatatcgatt tttatatttc
     3601 tttcgataat gaaaaagtcg gtgaactgca ggcaaaagcc ctggtcgata ttgttccgca
     3661 aggtaattac ttcctgatgg gcggctcgcc ggtagataac aacgccaagc tgttccgcgc
     3721 cgggcaaatg aaagtattaa aaccttatgt tgattccgga aaaattaaag tcgttggtga
     3781 ccaatgggtt gatggctggt taccggaaaa cgcattgaaa attatggaaa acgcgctaac
     3841 cgccaataat aacaaaattg atgctgtagt tgcctcaaac gatgccaccg caggaggggc
     3901 aattcaggca ttaagcgcgc aaggtttatc agggaaagta gcaatttccg gccaggatgc
     3961 ggatcttgca ggtattaaac gtattgctgc cggtacgcaa actatgacgg tgtataaacc
     4021 tattacattg ttggcaaata ctgccgcaga aattgccgtt gagttgggca atggtcagga
     4081 gccaaaagcg gataccacac tgaataatgg cctgaaagat gttccctccc gcctgctgac
     4141 accgatcgat gtgaataaaa acaacatcaa agatacggta gttaaagacg gattccacaa
     4201 agagagcgag ctgtaagcgt tacgccccgg cgcggagcgg gggcgtgatt tctctccatg
     4261 ccgcgtgaat tgattggctt aggtggagtc gttatgcctt atctacttga aatgaagaac
     4321 attaccaaaa ccttcggcag tgtgaaggcg attgataacg tcagcttgcg gttgaatgct
     4381 ggcgaaatcg tctcactctg tggggaaaat gggtcaggta aatcaacgct gatgaaagtg
     4441 ctgtgtggta tttatcccca tggttcctac gaaggcgaaa ttatttttgc gggagaagag
     4501 attcaggcga gtcacatccg cgataccgaa cgcaaaggta tcgccattat tcaccaggaa
     4561 ttggcgctgg tgaaagaact gaccgtgctg gaaaatatct tcctgggtaa cgaaataacc
     4621 cataatggca ttatggatta tgacctgatg acgctacgct gtcagaagct gctcgcacag
     4681 gtcagtttat ccatttcacc tgatacccgc gttggcgatt taggacttgg gcaacaacaa
     4741 ctggttgaaa ttgccaaggc acttaataaa caggtgcgtt tgttaattct cgatgagccg
     4801 acagcctcat taactgagca ggaaacgtcg gttttactgg atattattcg cgatctacaa
     4861 cagcacggta tcgcctgtat ttatatttcg cacaaactca acgaagtcaa agcgatttcc
     4921 gatacgattt gcgttattcg cgacggtcag cacattggca cgcgtgatgc tgctggaatg
     4981 agtgaagacg atattatcac catgatggtc gggcgagagt taaccgcgct ttaccctaat
     5041 gaaccacata ccaccggaga tgaaatatta cgtattgaac atctgacggc atggcatccg
     5101 gttaatcgtc atattaaacg agttaatgat gtctcgtttt ccctgaaacg tggcgaaata
     5161 ctgggtatcg ccggactcgt tggtgccgga cgtaccgaga ctattcagtg cctgtttggt
     5221 gtgtggcccg gacaatggga agggaaaatt tatattgacg gcaaacaggt agatattcgt
     5281 aactgtcagc aagccatcgc ccaggggatt gcgatggtac ccgaagacag aaagcgcgac
     5341 ggcatcgttc cggtaatggc ggttggtaaa aatattaccc tcgccgcact caaaaaattt
     5401 accggtagta ttagccagct tgatgacgcg gcagagcaaa aatgtattct ggaatcaatc
     5461 cagcaactca aagttaaaac gtcgtccccc gaccttgcta ttggacgttt gagcggcggc
     5521 aatcaacaaa aagcgatcct cgctcgctgt ctgttactta acccgcgcat tctcattctt
     5581 gatgaaccca ccaggggtat cgatattggc gcgaaatacg agatctacaa attaattaac
     5641 caactcgtcc agcagggtat tgccgttatt gtcatctctt ccgaattacc tgaagtgctc
     5701 ggccttagcg atcgtgtact ggtgatgcat gaagggaaac taaaagccaa cctgataaat
     5761 cataacctga ctcaggagca ggtgatggaa gccgcattga ggagcgaaca tcatgtcgaa
     5821 aagcaatccg tctgaagtga aattagccgt accgacatcc ggtggccttt ccgggctgaa
     5881 atcactgaat ttgcaggtct tcgtgatgat tgcagctatc atcgcaatca tgctgttctt
     5941 tacctggacc accgatggtg cctacttaag cgcccgtaac gtctccaacc tgttacgcca
     6001 aaccgcgatt accggcatcc tcgcggtagg aatggtgttc gtcataattt ctgctgaaat
     6061 cgacctttcc gtcggctcaa tgatggggct gttaggtggc gtcgcggcga tttgtgacgt
     6121 ctggctaggc tggcctttgc cacttaccat cattgtgacg ttggttctgg gactgcttct
     6181 cggagcctgg aacggatggt gggtcgcgta ccgcaaagtc ccttcattta ttgtcaccct
     6241 cgcgggcatg ttggcatttc gtggcatact cattggcatc accaacggca cgactgtttc
     6301 ccccaccagc gccgcgatgt cacaaattgg gcaaagctat ctacctgcca gcaccggctt
     6361 catcattggc gcgcttggct taatggcttt tgttggctgg caatggcgcg gaagaatgcg
     6421 ccgtcaggct ttgggtttgc agtctccggc ctctaccgca gtagtcggtc gccaggcttt
     6481 aaccgctatc atcgtattag gcgcaatctg gctgttgaat gattaccgtg gtgttcccac
     6541 tcctgttctg ctgctgacgt tgctgttact cggcggaatg tttatggcaa cgcggacggc
     6601 atttggacga cgcatttatg ccatcggcgg caatctggaa gcggcacgtc tctccgggat
     6661 taacgttgaa cgcaccaaac ttgccgtgtt cgctattaac ggattaatgg tagccatcgc
     6721 cggattaatc ctcagttctc gactcggcgc tggttcacct tctgcgggaa atatcgccga
     6781 gctggacgca attgcagcat gtgtgattgg cggcaccagt ctggcaggcg gcgtcgggag
     6841 cgtggctgga gcagtaatgg gggcattcat catggcttca ctggataacg gcatgagtat
     6901 gatggatgta ccgaccttct ggcagtatat cgttaaaggt gcgattctgt tgctggcagt
     6961 atggatggac tccgcaacca aacgccgttc ttgattttga taaaaatttt ctcaaagccg
     7021 gtaacgtatt accggttttg agtttttgca tgattcagca ggaaaagaac catgtttact
     7081 aaacgtcacc gcatcacatt actgttcaat gccaataaag cctatgaccg gcaggtagta
     7141 gaaggcgtag gggaatattt acaggcgtca caatcggaat gggatatttt cattgaagaa
     7201 gatttccgcg cccgcattga taaaatcaag gactggttag gagatggcgt cattgccgac
     7261 ttcgacgaca aacaaatcga gcaagcgctg gctgatgtcg acgtccccat tgttggggtt
     7321 ggtggctcgt atcaccttgc cgaaagttac ccacccgttc attacattgc caccgataac
     7381 tatgcgctgg ttgaaagcgc atttttgcat ttaaaagaga aaggcgttaa ccgctttgct
     7441 ttttatggtc ttccggaatc aagcggtaaa cgttgggcca ctgaacgcga atatgcattt
     7501 cgtcagcttg tcgccgaaga aaagtatcgc ggagtggttt atcaggggtt agaaaccgcg
     7561 ccagagaact ggcaacacgc gcaaaatcgg ctggcagact ggctacaaac gctgccaccg
     7621 caaaccggga ttattgccgt tactgacgcc cgggcgcggc atattctgca agtatgtgaa
     7681 catctacaca ttcccgtacc ggaaaaatta tgcgtgattg gcatcgataa cgaagaactg
     7741 acccgctatc tgtcgcgtgt cgccctttct tcggtcgctc agggcgcacg gcaaatgggc
     7801 tatcaggcgg caaaactgtt gcatcgatta ttagataaag aagaaatgcc gctacagcgg
     7861 attttggtcc caccagttcg cgtcattgaa cggcgctcaa cagattaccg ctcgctgacc
     7921 gatcccgccg ttattcaggc catgcattac attcgtaatc acgcctgtaa agggattaaa
     7981 gtggatcagg tactggatgc ggtgggcata tcacgctcca atcttgagaa gcgttttaaa
     8041 gaagaggtgg gcgaaaccat tcacgccatg attcatgccg aaaagctgga gaaagcgcgc
     8101 agcctgctga tttctactac cttgtcaatt aacgagatat cgcaaatgtg cggttatcca
     8161 tcgctgcaat atttctactc tgtttttaaa aaagcatatg acacaacgcc aaaagagtat
     8221 cgcgatgtaa atagcgaggt catgttgtag cgatgatgag aattatcggc gtcgcatcag
     8281 gtaactcagc acaaccacct gatacgagca aaagccttat ccgcctgaca agtcggataa
     8341 ggctcgagga aatgcaatta catatgcgcc gcgattaacc gttggttatc ctggtacatt
     8401 gcgaacaggt agttgttata actcttccct ttggtcgagt agcccttcag cttgtgaatc
     8461 atcgctgtgg cagtcacttc ctgatccgct ttacgcagtt gtgcacgcga tttacggaac
     8521 gaagagtaag ccgggtgcgt attgaggtta gtgacatagg cgctcaccga ttctttgaca
     8581 gaactaaact gtgagtaccc tttcacttta cccggcgcat tggtacaacg tcctttcatg
     8641 catttcatgc cgaacaggtt gttgttgttg cgcgccagct ttgacgttcc ccaaccgctt
     8701 tctgctgcag ccatcgtcgc caccatactg gtagggataa tgtctacgcg ttcaagcaag
     8761 gtattccacg ggatttttcg cgtattacca gaccacttca ccttgtagcg tttggcgatg
     8821 tctttcagac gcgcacgctc agcaggtgac cattggccct gatactgttt tgaaatcagc
     8881 cagttacgtt ccgcagtaat ggccgcattt tggctggtaa tgtaaggcat tacagtccgg
     8941 agaaacgcct ttttccttgg tgttccggaa gggtattttc gcaaatcagg aagtgaacta
     9001 ctctttgcac tattgcgaga atactcttgt ttactgctta cctgtttatt actagcttta
     9061 gttaagtggg acttttgact cgctgttgtt gtgtgcgtct tcgctaacac ctcactcgaa
     9121 aacaccagag tgagtaacat aagaatcatc gccccatatc gtcgtatggg agtcaaaatc
     9181 atcaggtctc ctggtcggat ttaatcattc caacacctta tattttttac aaatttgaga
     9241 gttgaatctc aaatcatatc aaaaatagct gtcaagagca ccccaaggaa tagtccaaat
     9301 ctgaaactat gtcacgagtt aacgattcag attggcgcta aatcgcagaa aatgtggggt
     9361 ttatcgcaaa attcagccgt tttttgcgcg cgatcgctca cccttgcttc tcatcctgtg
     9421 gacttaccgc tcagggatga gttttgtctg gcttatcgct ggcaaactgt ctgaaatcgc
     9481 agcaataagg actcatccgc catgaaactc gccgcctgtt ttctgacact ccttcctggc
     9541 ttcgccgttg ccgccagctg gacttctccg gggttccctg cctttagcga acaggggacg
     9601 ggaacatttg tcagccacgc gcagttgccc aaaggtacgc gtccactaat gctaaatttt
     9661 gaccaacagt gctggcagcc tgcggatgcg ataaaactca atcagatgct ttccctgcaa
     9721 ccttgtagca acacgccacc tcaatggcga ttgttcaggg acggcgaata tacgctgcaa
     9781 ctagacaccc gctccggtac gccaacattg atgatttccc tccagaacac cgtcgaaccg
     9841 gtagcaagcc tggtccgtga atgcccgaaa tgggatggat taccgctcac actggatgtc
     9901 agcgccactt ttgcggaggg agccgctgtc cgggattatt acagccagca aattgcgata
     9961 gtgaagaacg gtcaaataac gttacaaccc gctgccacca gcaacggttt actcctgctg
    10021 gaacgggcag aaactgacac atccgcccct ttcgactggc ataacgccac ggtttacttt
    10081 gtgctgacag atcgtttcga aaacggcgat cccagtaatg accagagtta cggacgtcat
    10141 aaagacggta tggcggaaat tggcactttt cacggcggcg atttacgcgg cctgaccaac
    10201 aaactggatt atctccagca gttgggcgtt aatgctttat ggataagcgc cccatttgag
    10261 caaattcacg gctgggtcgg cggcggtaca aaaggtgatt tcccgcatta tgcctatcat
    10321 ggttattaca cacaggactg gacgaatctt gatgccaata tgggcagtga agccgatcta
    10381 cggacgctgg ttgatagcgc gcatcagcgc ggtattcgta ttctctttga tgtcgtgatg
    10441 aaccacaccg gctatgccac gctggcggat atgcaggagt atcagtttgg cgcgttatat
    10501 ctttctggtg acgagctgaa aaaaacgctg ggtgaacgct ggagcgactg gaaacctgcc
    10561 gccgggcaaa cctggcatag ctttaacgat tacattaatt tcagcgacaa aacaggctgg
    10621 gataaatggt ggggaaaaaa ctggatcaga acggatatcg gcgattacga caatcctgga
    10681 ttcgacgatc tcactatgtc gctggccttt ttgccggata tcaaaaccga atcaactagc
    10741 gcttctggtc tgccagtgtt ctataaaaac aaaacggata cccacgctaa agacatcgac
    10801 ggctttaccc ctcgcgatta cttaacccac tggttaagtc agtgggtccg cgactatggg
    10861 attgatggtt ttcgggtcga taccgccaaa catgttgagt tgcccgcctg gcagcaactg
    10921 aaaaccgaag ccagcgctgc acttcgcgaa tggaaaaaag ctaaccccga caaagcatta
    10981 gatgacaaac ctttctggat gaccggtgaa gcctggggcc acggcgtgat gcaaagtgat
    11041 tactatcgcc acgacttcga tgcgatgatc aatttcgatt atcaggagca ggcggcgaaa
    11101 gcagtcgatt gtctggcgca gatggatacg acctggcagc aaatggcgga gaaattgcag
    11161 gatttcaacg tgttgagcta cctctcgtcg catgataccc gcctgttccg tgaagggggc
    11221 gacaaagcag cagagttatt attattagcg ccaggcgcgg tacaaatttt ttatggcgat
    11281 gaatcctcgc gtccgttcgg tcctaccggt tctgatccgc tgcaaggtac gcgttcggat
    11341 atgaactggc aggatgttag cggtaaatct gccgccagcg tcgcgcactg gcaaaaaatc
    11401 agccagttcc gcgcccgcca tcccgcaatt ggcgcgggca aacaaacgac actttcgctg
    11461 aagcagggct acggctttgt tcgtgagcat ggcgacgata aagtgctggt catctgggcg
    11521 gggcaacagt gacttttccg gcttcccgtt cgtgagtacc tcgggaagcc gccaaccagg
    11581 ataaaatgtc agccctaatc agcattgcag gataaagcac cgctcactct tcaacagacc
    11641 gatttgcacc ccagcaaatg tagcgttatt gttaccttcc ttgctacaga gttcgacaga
    11701 tatcccgcta tgacattctc cctttttggt gacaaattta cccgccactc cggcattacg
    11761 ctgctgatgg aagatctgaa cgacggttta cgcacgcctg gcgcgattat gctcggcggc
    11821 ggtaatccgg cgcagatccc ggaaatgcag gactacttcc agacgctact gaccgacatg
    11881 ctggaaagtg gcaaagcgac tgatgcactg tgcaattacg acggtccaca ggggaaaacg
    11941 gagctactca cactgcttgc cggaatgctg cgcgagaagt tgggctggga tatcgaacca
    12001 cagaatattg cactaacaaa cggcagccag agcgcgtttt tctacttatt taatctgttt
    12061 gccggacgcc gtgccgatgg tcgggtcaaa aaagtgctgt tcccgctggc accggaatat
    12121 attggctacg ctgacgccgg actggaagaa gatctgtttg tctctgcgcg tccgaatatt
    12181 gaactgctgc cggaaggcca gtttaaatac cacgttgatt ttgagcatct acatattggc
    12241 gaagaaaccg ggatgatttg cgtctcccgg ccgacgaatc caacaggcaa tgtgattact
    12301 gacgaagagt tgctgaagct tgacgcgctg gcgaatcaac acggcattcc gctggtgatt
    12361 gataacgctt atggcgtccc gttcccgggt atcatcttca gcgaagcgcg cccgctatgg
    12421 aatccgaata tcgtgctgtg catgagtctt tccaagctgg ggctacctgg ctcccgctgc
    12481 ggcattatca tcgccaatga aaaaatcatc accgccatca ccaatatgaa cggcattatc
    12541 agcctggcac ctggcggtat tggtccggcg atgatgtgtg aaatgattaa gcgtaacgat
    12601 ctgctgcgct tgtctgaaac agtcatcaaa ccgttttact accagcgtgt tcaggaaact
    12661 atcgccatca ttcgccgcta tttaccggaa gatcgctgcc tgattcataa accggaagga
    12721 gccattttcc tctggctatg gtttaaggat ttgcccatta cgaccgagca gctctaccag
    12781 cgcctgaaag cacgcggcgt gctgatggtg ccggggcaca acttcttccc gggactggat
    12841 aaaccgtggc cgcatacgca tcaatgtatg cgcatgaact acgtaccaga gccggagaaa
    12901 attgaggcgg gtgtgaagat tctggcagaa gagatagaaa gagcctgggc tgaaagtcac
    12961 taaaaattgc ctgatgcgct acgcttatca ggcctacatg atttctgcaa tatattgaat
    13021 ttggtagaat ttgtaggccg gataaggcgt tttagccgca tccggcataa acaaagcgca
    13081 ctatccctgc gttgagaaac gccggaaaaa cgtttcaaac aggctgctgc cgtagccgat
    13141 gtcgctgcac tttctcgaca tccatgcact gcaacgcctt tgtcgggcag gcttcaacac
    13201 aggccggccc cgtcgcccga tgccagcaca gatcgcactt aatcgccctc gcctttttgc
    13261 gcgaagaaac gacctccatc gcaccaaacg ggcaagccag catacagctt ttacagccaa
    13321 tgcaacgtga ttgttcaacg aaaatatgcc catgttcgcg gcttatcgcg tcaacagggc
    13381 agacattcgc gcacggtgca tcttcacact gatgacaggc tactgccgtg gtccagctgt
    13441 ggtctttaat gacacgaata cgggaaataa actcgtctgg tgacaacgca gcgcaatcct
    13501 gattctcctg atgcgacact gcgcaagcca cttcacaggt acggcaaccg atacatttcg
    13561 tcgcatccgc aataataaac cggttcatga tcactccttt gcactaaaac agcagagtgc
    13621 cagagagcgc gtccctactg ctttgatcca acgaaggaaa tgttaaagtt ttggcacttg
    13681 tccaaagggt tatgtaatcg cgccctggtc gtccctgaca gtaaatccca gttcattaga
    13741 aatagcctgc gcggtttcac gcagtggttt caggagattt ttctctccca cctgtttcag
    13801 acgtgatgtc gaaagcgaaa tcgacacggc gtacggcacc cgcccatgaa tatcaaacac
    13861 cggaacagca atacaggaga cgccgagttc gttttcttct ctgtccatcg ccgctccact
    13921 ttcacgaatg tgcgccagtt cgtcgaacat cgcgggcagc tcggtaatgg tattgcgggt
    13981 taacggctgg atctcatgct gatggctttc ccagtatgac ttcacgtagt ccgggtgacc
    14041 aaacgccatg tagatcttgc ccattgcgga acagtagagc ggcatatgct ggcctatata
    14101 ggcacgggtt cgcagcatcc cggttgtggg ttccagctta taaatcaaaa tagcgtgatc
    14161 gtcttcgcgg ctggagaagt taatagtttc accagtggcg atgttcagag cctcaagatg
    14221 cggagcggcg atatgaatga tattcagcga agatagcgcc ttctgcccga cggcaataaa
    14281 tttggtggtc aggcgataac tccctgcggc gggcgcggtg gtcacatagc cgcaggactg
    14341 taatccctgc aataagcgat ggacggtact cttatttaaa ccagccagct ccgaaagatg
    14401 cgccaacgga caaccgtttg gatagttgct caaaatctca atcagcatca acccacgaaa
    14461 cagactctgg cttccggctg ggcgctcttt ttcctgcgcc atctcgttct ctttttttcc
    14521 catcacttct ttccccattt tgtcgcgtcc tgatggtagc gcaaagtgtg ccgtagttca
    14581 cgatctcgac agataattta taaccacttg atttttaagt tttttgaaat tcatcaatca
    14641 gattgctttg ttaaaaagtg atcgatatat ttgaaatcaa gtttcgtata ttgaaatttt
    14701 aagccaaaaa agcgatcaaa aaaacaagga agcctgggat gaaagtgaca tttgagcagt
    14761 taaaagcagc ctttaatcgg gtcttaattt cacgcggcgt tgacagcgaa acggctgacg
    14821 cctgtgcaga gatgttcgcc cgcaccaccg aatcaggcgt ttattctcac ggcgttaatc
    14881 gtttccctcg tttcattcaa caactggaaa acggcgatat cattcctgat gcccaaccca
    14941 aacgtataac cagcctcggc gcaattgaac agtgggacgc ccagcgttcg atcggtaacc
    15001 tgacagcgaa aaagatgatg gatcgcgcca ttgaactggc tgccgatcac ggtattggtc
    15061 tggtggcact acgtaatgcc aaccactgga tgcgcggcgg cagctacggc tggcaggcgg
    15121 cggaaaaagg ctatattggc atttgctgga ccaactccat cgccgtaatg ccaccgtggg
    15181 gcgcaaaaga gtgtcgcatc ggcaccaacc cgctgatcgt cgccattcct tccacgccga
    15241 tcaccatggt cgatatgtcg atgtcgatgt tctcttacgg catgttagaa gttaatcgcc
    15301 tggctggtcg tcagctcccg gtagatggtg gctttgatga tgagggcaat ttgaccaaag
    15361 aacctggcgt tatcgagaaa aatcgccgca ttttgccgat gggctactgg aaggttccgg
    15421 tatgtcgatt gtgctggata tgatcgctac tctcctttct gacggcgcat cggttgccga
    15481 agtcaccgag gacaacagcg acgaatacgg catttcacaa atttttattg ccattgaagt
    15541 ggataagctt atcgacggtc ccacccgcga tgccaaactg caacgcatca tggattacgt
    15601 taccagtgcc gagcgtgctg acgaaaatca ggccattcgc ttacctggcc acgaatttac
    15661 taccctgctg gcagaaaacc gccgtaacgg tattaccgtt gatgacagcg tgtgggcaaa
    15721 aatccaggcg ttataaggag ataagccatg atttttggac atatcgcgca gcctaatccg
    15781 tgccgtttgc ccgccgccat tgaaaaggcg ctcgattttc tgcgcaccac cgacttcaac
    15841 gccctggaac cgggcgttgt cgaaatcgac ggcaagaata tttatgcgca aattatcgat
    15901 ttaaccacac gcgaagcggt ggaaaaccgt ccggaagtgc atcgtcggta tatcgatatt
    15961 cagtttctgg cgtggggtga agagaaaatt ggcattgcca ttgatacggg aaataataaa
    16021 gtcagcgaat cactattaga gcagcgcgat attatttttt atcacgatag tgaacatgaa
    16081 tcatttatag aaatgatacc gggcagctac gctattttct tcccgcagga tgttcatcgc
    16141 ccggcatgta ataaaaatgc cactacggca atacgcaaga tcgtggttaa agtggccctc
    16201 gctgctttat aaaaaatgat tactggagca cataatgaaa acgaataatg ccggttatat
    16261 tatcggcgcg tatccctgtg caccttcttt tcaccaaaga agtgaagagg aagagaagga
    16321 tttctggcga caattatctg agactccaga cattttgggt ctggaacaac cttgcctgga
    16381 aaattttcac cctcttggcg atcaatggtt actccgtcat acgccagaga gctggaaatt
    16441 tgtcgtcacc gcagtgatgg aaaccatgcg tcgccgcagc gaaaacagcg ggtttggtct
    16501 cgcctccagt gatgaagaac agcgccaggc ctgcgtggcg tactatcgcc acctgttcaa
    16561 taaaatcaac gcattgcagg ctaataaagt gctcgcactg gagctccagg ctgcaccgtt
    16621 agcaacgaat cctaatgtta tgcaggcgac ggacgctttt gcccgctcat tgaaagaaat
    16681 cgccagttgg gactggccgt gcaagctggt gctggaacac tgcgacgcca tgacatcttc
    16741 ttccccccga aaaggctttt tgcctttaga aaatgtgctg gaagttatta ccgattatga
    16801 catcagcatc tgtatcaact gggcgcgctc tgccattgaa gggcgcaata caacgctgcc
    16861 gctgacccac acccaaatgg cgaaacaagc cggaaaactc ggcgcgttaa tgttctctgg
    16921 tacgacgcta aacggggcat acggcgaatg gcaggattta cacgcaccgt ttgcaccatt
    16981 ctgcgcagaa agcctgatga ccacagatca tgttcgcgag ttatttaatg tagcagaatc
    17041 gtcaacattg cattttgctg gcatcaagct actggaaata aatgcgacgg ctgatgtgca
    17101 ccatcgcatc gaaatattac gtaacggcat tcactctctg aatgaatctc gttaaatagt
    17161 tccgcttata tatcactttg aatatctaat tagccgtttt ctgataatta ttacgaatat
    17221 taacaacatt agcgcataaa aaatattgca taaccagaag gactcgttat gaaacggata
    17281 ctcgaaggca ttcttgcgat catcatcgca atactgtctt gtattatttt tattaatatt
    17341 attcttcgat atggattcca cacaagtata ttatcgattg atgaattatc ccgactttta
    17401 tttgtctggt taacctttat tggcgcaatc gttgcttata tggacaacag ccatgtgcag
    17461 gttacttttt tagtcgaaaa actctcaccg gctaatcaac aacgtgtttc gctgctaacc
    17521 catacgctca ttttattgct gtgtctgggg ctggcctggg gcgctataga aaaaacagcg
    17581 caagactgga gcaacttatc gcccattctt ggcgtccccg ttggcttaat gtacgccgcc
    17641 gctatcccca ccagtctgat tatcgcattg ctggaattgc gtcacttata ccggcagttc
    17701 acaaataccc ccagccgaaa ccaacaagga gcctgacgat gacaatcctt atatttattg
    17761 tcagtttgtt aggggctatc gcaattggcg ttcccatcgc ctgggcatta ttactgtgcg
    17821 gcatttcatt gatgttctgg atggatatct ttgatgtgca aattctggca caaacgttgg
    17881 taaatggcgc agacagcttc tccctgttag ccatcccctt ctttgtactg gcgggtgagc
    17941 tcatgaacgc gggcggcctg tcgcaacgta tcgtcgactt gccaatgaaa ctggtaggcc
    18001 acaggctagg cggtctgggt tacgtgggcg tgctggcagc gatgatcatg gcaagcctgt
    18061 ccggttctgc ggttgctgat accgcagctg tcgcggcgct gctggtgccg atgatgcgcc
    18121 aggccaacta tccggtaaac cgcgccgccg ggcttatcgg ttccgggggg attattgcgg
    18181 ccattatccc gccttccata ccactcatta tcttcggcgt ctccagcgga ctctccatca
    18241 gcaaactgtt tatggcgggc attgcgccag gcatcatgat gggcgtcacg ctgatggtta
    18301 cctggtggtg gcaggcaaaa cgactgaatt taccttgtca gccgaaagcc tcactgcgag
    18361 aagtctggca atccttagtt tccggtattt gggcgctctt tttaccgata attattattg
    18421 gcggtttccg ttcggggtta tttacaccga ccgaagcagg cgcggttgcc gccttctacg
    18481 ctctgttcgt ctcggtggtg gtctatcgcg agatgacctt ctccacgctt tatcatgtgc
    18541 tgattaacgc ggcaaaaacc acatccgtgg tgatgttcct ggtggcctct gctgcggtat
    18601 ctgcatggct tcttacgatc gccgaattac ccatgatggt atcggagcta ttacaaccgt
    18661 tggttgattc gcctcgctta ttattcatcg tcattatgct cgctattatg gtgatcagta
    18721 cggtcatgga cttaacgcct accgtattaa ttcttacacc agtattaatg ccattagtga
    18781 aagaagcggg aatagatccg gtctatttcg gcatcatgtt tattatcaac tgttcaatat
    18841 cactgattac gcctccagtc ggtaatgttc ttaacgtggt atgcggcgtg gcgaaactta
    18901 aatttgacga tgcggtaaaa ggcgttgcgc cttacgtcat ggttttattc atgttgttag
    18961 cattatttat ttttataccg gagctcatta ccgctcctct taaatggatg agttaaaagg
    19021 aaatcataat gaaattacgt tctgtaacct acgctttatt aattgccgga gtggcgacgt
    19081 tcagcacatc ttctctggcg gcacaatcat tacgtttcgg ttatgaaacc tcacaaaccg
    19141 attcacaaca tattgcggcg aaaaaattta atgaattact gcaggaaaaa accaaaggcg
    19201 aactgaaatt aaaactgttc ccggatagca cccttggtaa tgcgcaggcg atgatcagcg
    19261 gcgtgcgtgg cggcactatt gatatggaga tgtctggctc taacaacttt acgggactct
    19321 cgcccgtcat caacctgctc gatgtcccgt tcctgttccg cgataccgct cacgcgcata
    19381 aaacgctcga cggcaaagtc ggtgatgacc tgaaagtctc ccttgaaggt aaggggctga
    19441 aagtactggc ctactgggaa aacggctggc gcgatgtcac caactcgcgt gcaccggtca
    19501 aaacgcctgc cgacctgaaa gggttgaaaa tccgcaccaa caacagcccg atgaatatcg
    19561 ctgcatttaa agtttttggc gctaacccga tcccgatgcc gtttgccgaa gtctataccg
    19621 ggctggaaac ccgcactatc gacgctcagg aacacccgat caacgtcgtc tggtcagcaa
    19681 aattttatga agtgcagaag tacctttccc tgacccatca cgcctattcc ccgcttctgg
    19741 tggtgatcaa caaagcgaag tttgatggct taacgcctga gttccagcag gcactgatct
    19801 catctgcgca ggaagcgggt agctatcagc gcaaactggt cgccgaagat cagcaaaaaa
    19861 tcatcgacgg tatgaaagaa gcgggcgtgg aagtcatcac cgatctcgac cgcaaagcct
    19921 ttagcgatgc gctgggtacc caggtccgtg acatgtttgt taaagatgtt ccccagggtg
    19981 ctgatctgct gaaagccgtg gatgaggtgc aataaaccat gacgcaatac tggctggggt
    20041 tagattgtgg cggtagctgg ctgaaagccg ggctgtatga ccgagaaggc cgggaggcgg
    20101 gcgtgcagcg cctgccgctg tgcgcattaa gtccacagcc aggctgggca gagcgcgaca
    20161 tggcagaact gtggcaatgt tgcactgctg tcattcgcgc cctgcttacc cattctggcg
    20221 tcagcgggga acaaattgtc ggtatcggca tctccgcgca gggaaagggc ttatttctac
    20281 ttgataaaaa cgacaaaccg ctcggtaacg ccattttgtc ctcggatcgc cgggcgatgg
    20341 aaatcgttcg tcgctggcag gaagatggca tcccggaaaa actctatccg ctgacccgac
    20401 aaaccttatg gaccgggcat ccggtgtcgc tgttacgctg gctgaaagag cacgaaccag
    20461 aacgctacgc gcaaattggc tgcgtgatga tgacgcacga ctacctgcgc tggtgtttaa
    20521 ccggcgtcaa aggttgcgaa gagagcaata tttctgaatc caatctctac aacatgagtc
    20581 gcggggaata tgacccgtgc cttaccgact ggctggggat tgctgaaatc aatcacgccc
    20641 tgccgcctgt tgtcggatct gccgaaatct gcggggaaat caccgctcag acagccgtac
    20701 tgaccggtct gaaagcgggt acccccgttg tcggcggcct gtttgatgtg gtttccaccg
    20761 cactctgcgc cgggctcgaa gacgaattca ccctcaatgc ggtgatgggg acctgggcgg
    20821 tgaccagcgg cataacccac ggtttacgtg acggtgaagc gcatccgtat gtctatggtc
    20881 gctacgttaa cgatggtcaa tttatcgttc acgaagccag ccccacctct tccggcaatc
    20941 tcgaatggtt taccgcacag tggggagaaa tctcatttgc tgagattaat caggccgttg
    21001 ccagtttgcc gaaagccggt ggcgatctct ttttcctgcc gttcctgtac ggcagcaacg
    21061 ccggactcga aatgaccagc ggtttctacg ggatgcaggc cattcacacc cgtgcacacc
    21121 tcttacaggc catctatgaa ggcgtggtgt tcagccatat gactcatctc aaccggatgc
    21181 gcgaacgttt tactgatgtg cacaccctgc gcgtcactgg cggcccggcg cactccgatg
    21241 tctggatgca aatgctggcg gacgtcagcg gtctgcgtat cgagctgccg caggtggaag
    21301 aaaccggctg ctttggtgcg gcacttgccg cccgcgtcgg taccggggtt tatcgcgatt
    21361 tcagcgaagc ccaacgtgac ttacagcacc cggtacgcac cctgctgccg gatatggccg
    21421 cgcatcagct ttaccaacaa aaataccaac gctatcaaca tctcattgcc gcacttcagg
    21481 gttatcacgc ccgcatcaag gagcacacat tatgagtcga ccacttctgc aactggccct
    21541 cgaccactca tcaattgaag ccgcgcagcg cgacgtgacg cggttaaaag acagcgtcga
    21601 tattgtcgaa gcaggcacca ttctctgttt aaacgaaggg cttggcgccg tgaaagcctt
    21661 gcgcgaacag tgccctgaca aaatcatcgt tgctgactgg aaggtcgccg acgcgggtga
    21721 aacgctcgcg caacaggcat ttggcgcagg cgctaactgg atgaccatca tctgcgccgc
    21781 gccgctcgcc acggtagaaa aaggccacgc gatggcacag aactgcggtg gtgaaattca
    21841 gatcgagcta ttcggtaact ggacgctgga cgatgcccgt gactggcatc gtattggcgt
    21901 gcggcaggcc atttatcatc gtagtcgtga cgcgcaggcc agcgggcaac agtggggcga
    21961 ggccgatctg gcacgcatga aggcgctttc cgatatcggc cttgagcttt ccattaccgg
    22021 cgggattacc cctgctgacc tgccgctgtt taaagagatc cgcgtgaaag cgtttattgc
    22081 cggacgggcg ctggcaggcg cagcaaatcc ggcgcaagtg gctggcgatt tccatgcgca
    22141 aatcgactcc atctggggag gcaagcgtgc gtaatcatcc gttaggtatt tatgaaaaag
    22201 cgctggcgaa agatctctcc tggccggagc ggctggtgct ggcaaaaagc tgtggctttg
    22261 attttgtcga aatgtcggtg gatgaaaccg acgaacggtt gtcacgtctt gactggagcg
    22321 ccgcacaacg tgcttcgctg gtcactgcga tgatcgaaac gggtgttgct attccgtcaa
    22381 tgtgcctgtc cgcacatcgt cgttttcctt ttggtagccg ggacgacgcc gtgcgccagc
    22441 gggcgcggga gattatgagt aaagcgattc gcctggcgcg cgatctcggc attcgtacta
    22501 ttcaactggc aggttacgac gtctattacg aggatcatga cgaaggcacc cagcaacggt
    22561 ttgctgaagg gctggcgtgg gcggttgaac aggcagcggc atcacaagtc atgctggcgg
    22621 tggagattat ggacaccgcg tttatgaact ctatcagcaa atggaaaaag tgggacgaaa
    22681 tgctcgcctc gccgtggttc accgtctacc cggacgtcgg caacctcagc gcctggggca
    22741 atgatgttcc cgccgaactg aaactgggca tcgaccgtat cgccgccatc cacctgaaag
    22801 acactcaacc cgttaccgaa catagccccg gacagttccg cgatgtgccg tttggcgaag
    22861 gctgtgtcga ttttgttggc atcttcaaaa cgctgcatga gctgaactat cgcggatcat
    22921 ttctgattga aatgtggacc gaaaaagcca aagagccggt gctggagatt attcaggcgc
    22981 ggcgctggat tgaagcgcgt atgcaggagg ccggatttat atgttagagc aactgaaagc
    23041 tgacgtgctg gcggcaaacc tggcgcttcc cgttcatcat ctggtgacgt tcacctgggg
    23101 caatgtcagc gcgatagacg aaacgcggca atggatggtg attaagcctt ccggcgttga
    23161 gtatgaggta atgaccgccg acgatatggt ggtggttgag atagccagcg gcaaggtggt
    23221 ggaaggcagc aaaaagccct cttccgacac gccaacgcat ctggcgcttt accgtcgctt
    23281 tgcggagatt ggcggcatag tgcataccca ctcgcgccac gccaccatct ggtcgcaggc
    23341 cggacttgat ctccccgcct ggggcactac tcacgccgat tatttttacg gagccatccc
    23401 ctgcacgcgc ctgatgaccg cagaagagat caacggcgag tatgaatacc agaccggcga
    23461 agtgatcatt aaaaccttcg aagagcgtgg gttgaacccg gcacaaatcc cggcggtact
    23521 ggtgcattct cacggcccgt tcgcctgggg aaaaaatgcc gccgatgcgg tgcataacgc
    23581 cgtggtactg gaagagtgcg cctatatggg tctgttctcg cgccagcttg cgccacagct
    23641 ccctgcgatg caaaacgaac tgctggataa gcactacctg cgtaagcatg gggccaatgc
    23701 ctattacggg cagtagccat gcctgagagt gcccggcaat acacgccggg cagcctttca
    23761 catcagaaca gccccaacgg tttatccgag tagctcacca gcaggcactt ggtttgctgg
    23821 taatgctcaa gcatcatttt gtgggtttcg cggccaatgc ctgattgttt gtagccgcca
    23881 aacgccgcat gtgccgggta agcgtgataa cagttggtcc acacgcgccc ggcctgaatg
    23941 ccgcgcccca tcttataggc cagattaccg ttgcggctcc agacgcccgc gcccaggcca
    24001 tattgcgtat cgttcgccag ctccagcgct tcttccatcg ttttgaaggt ggttaccgcc
    24061 agcaccgggc caaaaatctc ctcctggaac acgcgcatat tgttctgacc aaacaaaatc
    24121 gtcggttcga ggtagtagcc gtctttcagt tcgccttcca gcagcttgcg ccgcccgccg
    24181 gtgagcacat cagcgccctc ttttttaccg atatcaatgt agttgaggat ggtttccagt
    24241 tgcccgtgag aaacctgcgc gcccatttgc gtcacgctgt cgagcgggtt accgctgcga
    24301 atgctttcga cacgacggat ggcgcgttcc ataaagcgtt cgtagataga ctcctgcact
    24361 aaagcacggc ttggacaggt gcaaacttcg ccctggttaa aggcaaacag tgcaaagcct
    24421 tccagcgctt tatcgaaaaa ggcatcttct tcatccatca catcagcaaa gaagatattt
    24481 ggcgatttgc cgcccagctc cagcgtcacc ggaataatgt tttgcgtggc gtattgcata
    24541 atttgttggc ccacttccgt tgagccagta aacgccactt tggcgatgcg tttcgaggtc
    24601 gccagatatt cgccaatttc cccacctgcg ccattgacca cgttcaccac ccctggcggc
    24661 agtaaatcac cgacgatttc cattagcagc agtacagaaa gcggagtaag acgtgcgggt
    24721 ttcagcacca cacagttgcc agccgccagc gcgggagcca ttttccagct cgccatcagc
    24781 agcgggaagt tccacgggat aatctgcccc accacgccta acggctcatg gaaatgatag
    24841 gccacggttt cgctatcaat ttcactgatc ccaccttcct gcgcccgaat acacgaggca
    24901 aaatagcgaa aatgatcaat cgccagcggc acgtcggcag cactggtttc gcgaatgggt
    24961 ttaccgttat cccaggtttc tgccgtcgct aaaagttcaa ggttttgttc cattcgatcg
    25021 gcaatcttaa acagaatcgc cgcacgatcc tgcaccgagg tgtgcgccca tttatctttc
    25081 actttgtgcg cagcatccag cgccagatcg atgtctcgtt tgcccgaaga cgccacttcg
    25141 cacagtagct gcccggtcac cggcgtcagg ttctggtaat actcgccatc ggcaggcgct
    25201 acccattcgc caccgataaa gttgtcgtag cgggctttta atttgagggg gaaaccatac
    25261 tcgccgggct gaacacgtgc tgaaggggga ttattggtca tgatcgtctc cttgcggtgt
    25321 gaggtataac aagggtagac gtaactggcg aaatcttcgc cagtcggtga cagctttacg
    25381 acggctatca cgaatttacg ggcaagggtt acagcggata atgcgcgaag tacacggctt
    25441 gctggtcatt aattttcttc tcttgcgcaa acccttcttt aacaattgtc cgtaaatcag
    25501 ctcgagccgt attatcagag acgcccaagc tcgcgctaat ttcagcaact gtgtactctt
    25561 tccccggatt ggcgagcatc acattcagga gcgttacctg gcgcggattc aggcgtttaa
    25621 gcacaccaga gctaaacagc acactatcga gttttgcgct tcgttgtgta atttcatcga
    25681 tatgctgctg ccagttttgc agagcacgtt taatgacacc cgcctgatat tcaaggaaat
    25741 aagtcagatc cataccatct gattcagtgt actggtagct ggcggcatat ttgaccggtg
    25801 cagcatgaag caaacggctg atggaaatat atttaaagac atcgtagccc gatttcagca
    25861 tataccagta aaacagcgcc cgactggtgc gcccgttgcc atcccgaaac ggatgttcat
    25921 gggccagcat aaaatgcaga atgcaggcac ggattaacgg gtgaatatac ccttcatggc
    25981 tgtttaacca ctggcaaact ttttccagcc gttccggtaa caatgccgca gcgggcggtt
    26041 gatgcactat attgccatca tagtcggcga tcacaacatc atctgtttca cgaaattctc
    26101 ccggacgata tttcgcgtca ttgattccac ccataccaat ggcatgtaac tggcgaataa
    26161 gcgcaggtgt cagcggttct gcgagtaagt gcggaatttc cgccatcagc cgggcatttc
    26221 ccgcaatcat atgctcgtct tcagtgcgtg gcgaccgccc tgtttccagc atttttctcg
    26281 ccaccagcgt tgtagtattc gcaccttcca gctgggagct ggtgatcggc tcttccaggc
    26341 gtaactggct taactcggct ccggccccgc gtaaccgttc agtctgatct ttcagcgcca
    26401 gcgcggtagc acgtttgtcg acaagctcgc acgcttctgc aatcaccggg gtaatgttga
    26461 atccggcctg ttcacccgct tcattgcaat aattaattcg ttggatcgcc gcgttgcgcg
    26521 ccagacgggt aagcgtccac gccacatcga cgttttctcc ttttttgatg cggtactgca
    26581 actcatcaaa aggcagataa cgtccttttt catcggtaac ccggttaaag cgcaggtggg
    26641 ctaggagttc ctcacccgat aagtgtgata aaggaataag tccgggaggt gatttgatag
    26701 ccatgtgctt atctcaaaaa agcgcaatat tgagatagtg tatagcagga aagagaaact
    26761 atctcaaaaa aagcaagttt tgagatagtt gaaaaagcgg ctgacaactc gccagccgtt
    26821 gtgggaatga tgattacatt gcggcgcgat aaatcgccac aatttcttcg tgagttgcct
    26881 ggatcgggtt agtaaagcca caggcatctt tcagggcatt agtcgccagt accgcgaaat
    26941 cttcttcttt cacgttcagg tcgcgtaggc ctgccgggat atccactttc ttcgccagtt
    27001 cacggatggc gttaatgcag gcttcagcac cttccgcgtc gtttttacca gtcacgttca
    27061 cgcccattgc agcggcacag tcacgcagac gtgctgcagc gactttgcta ttgaatacct
    27121 gaacgtgtgg cagcagaaca gcattacata caccgtgtgg caggttgtag aaaccgccca
    27181 gctggtgcgc catcgcatga acataaccca gagaagcatt gttgaacgcc ataccggcga
    27241 ggaactgggc ataagccatt gcttcacgcg ctttcgcatt actaccatct tcaacggcta
    27301 acggcaggtt ttcggcaatc atggtcacgg ctttcagtgc gcaagcgtca gtgatcggcg
    27361 tggcggcaat agaaacatac gcttcgatag cgtgcgttaa ggcatccata cccgttgcgg
    27421 cggtcagtga cttcggcata ccgatcatca gagaggagtc attgacagaa agcaacggag
    27481 ttacatgttt atccacaatc gccattttga tatgacgcgc ttcgtcagtg atgatgcaga
    27541 aacgggtcat ttcagaggcc gtacccgccg tggtgttgat ggcgatcatc ggcagctgcg
    27601 gttttgcaga gcggtcaacg ccttcataat cgcggatgtc accgccgttg gctgcaacca
    27661 gcgcaatacc ttttgcgcag tcgtgtggag aaccaccgcc taaggaaatc acgctatcgc
    27721 aattattttc tttaagtaat ttcaaacctg cagcgacgtt ttccgtggtg gggttaggtt
    27781 gggtgccatc ataaataacg ctaaaaatat tgcgttcttc cagtgctttt tgcacatcac
    27841 ccgccatacc caatttcgtt aacatgttgt cggtgacaat taaggtacgg gtaaatccat
    27901 aatctgccat catattcatt gcatcattca atgaatcagc accaataaca ttcacggaag
    27961 gaataaaaaa ggttgaagct gccataacac actcctgaaa gtgtaaaaaa gggcgggcag
    28021 cttactgagg attttcattt gaaaatatga tcaacattac cgtttattaa aata
//
LOCUS       DADTFG010000060        26699 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.060, whole genome shotgun sequence.
ACCESSION   DADTFG010000060 DADTFG010000000
VERSION     DADTFG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.060"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            259..1728
                     /gene="gspA"
                     /locus_tag="J1138_002285"
     CDS             259..1728
                     /gene="gspA"
                     /locus_tag="J1138_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HBA8454078.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTRVFT
                     AKQWRMIYRVLGDNRLRKMQLAVVMSGTIIALTCGWLLLSSFTAPLPVPAWLIPVTPV
                     AKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1730..2149
                     /gene="gspB"
                     /locus_tag="J1138_002286"
     CDS             1730..2149
                     /gene="gspB"
                     /locus_tag="J1138_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HBA8454079.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDEPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2387..2698
                     /gene="rpsJ"
                     /locus_tag="J1138_002287"
     CDS             2387..2698
                     /gene="rpsJ"
                     /locus_tag="J1138_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBA8454080.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2731..3360
                     /gene="rplC"
                     /locus_tag="J1138_002288"
     CDS             2731..3360
                     /gene="rplC"
                     /locus_tag="J1138_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBA8454081.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3371..3976
                     /gene="rplD"
                     /locus_tag="J1138_002289"
     CDS             3371..3976
                     /gene="rplD"
                     /locus_tag="J1138_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBA8454082.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3973..4275
                     /gene="rplW"
                     /locus_tag="J1138_002290"
     CDS             3973..4275
                     /gene="rplW"
                     /locus_tag="J1138_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBA8454083.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4293..5114
                     /gene="rplB"
                     /locus_tag="J1138_002291"
     CDS             4293..5114
                     /gene="rplB"
                     /locus_tag="J1138_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBA8454084.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5131..5409
                     /gene="rpsS"
                     /locus_tag="J1138_002292"
     CDS             5131..5409
                     /gene="rpsS"
                     /locus_tag="J1138_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBA8454085.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5424..5756
                     /gene="rplV"
                     /locus_tag="J1138_002293"
     CDS             5424..5756
                     /gene="rplV"
                     /locus_tag="J1138_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBA8454086.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5774..6475
                     /gene="rpsC"
                     /locus_tag="J1138_002294"
     CDS             5774..6475
                     /gene="rpsC"
                     /locus_tag="J1138_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBA8454087.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6488..6898
                     /gene="rplP"
                     /locus_tag="J1138_002295"
     CDS             6488..6898
                     /gene="rplP"
                     /locus_tag="J1138_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBA8454088.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6898..7089
                     /gene="rpmC"
                     /locus_tag="J1138_002296"
     CDS             6898..7089
                     /gene="rpmC"
                     /locus_tag="J1138_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBA8454089.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7089..7343
                     /gene="rpsQ"
                     /locus_tag="J1138_002297"
     CDS             7089..7343
                     /gene="rpsQ"
                     /locus_tag="J1138_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBA8454090.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7508..7879
                     /gene="rplN"
                     /locus_tag="J1138_002298"
     CDS             7508..7879
                     /gene="rplN"
                     /locus_tag="J1138_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBA8454091.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7890..8204
                     /gene="rplX"
                     /locus_tag="J1138_002299"
     CDS             7890..8204
                     /gene="rplX"
                     /locus_tag="J1138_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBA8454092.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8219..8758
                     /gene="rplE"
                     /locus_tag="J1138_002300"
     CDS             8219..8758
                     /gene="rplE"
                     /locus_tag="J1138_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBA8454093.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8773..9078
                     /gene="rpsN"
                     /locus_tag="J1138_002301"
     CDS             8773..9078
                     /gene="rpsN"
                     /locus_tag="J1138_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBA8454094.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9112..9504
                     /gene="rpsH"
                     /locus_tag="J1138_002302"
     CDS             9112..9504
                     /gene="rpsH"
                     /locus_tag="J1138_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBA8454095.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9517..10050
                     /gene="rplF"
                     /locus_tag="J1138_002303"
     CDS             9517..10050
                     /gene="rplF"
                     /locus_tag="J1138_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBA8454096.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10060..10413
                     /gene="rplR"
                     /locus_tag="J1138_002304"
     CDS             10060..10413
                     /gene="rplR"
                     /locus_tag="J1138_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBA8454097.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10428..10931
                     /gene="rpsE"
                     /locus_tag="J1138_002305"
     CDS             10428..10931
                     /gene="rpsE"
                     /locus_tag="J1138_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBA8454098.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10935..11114
                     /gene="rpmD"
                     /locus_tag="J1138_002306"
     CDS             10935..11114
                     /gene="rpmD"
                     /locus_tag="J1138_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBA8454099.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11118..11552
                     /gene="rplO"
                     /locus_tag="J1138_002307"
     CDS             11118..11552
                     /gene="rplO"
                     /locus_tag="J1138_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBA8454100.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11560..12891
                     /gene="secY"
                     /locus_tag="J1138_002308"
     CDS             11560..12891
                     /gene="secY"
                     /locus_tag="J1138_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBA8454101.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12923..13039
                     /gene="rpmJ"
                     /locus_tag="J1138_002309"
     CDS             12923..13039
                     /gene="rpmJ"
                     /locus_tag="J1138_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBA8454102.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13186..13542
                     /gene="rpsM"
                     /locus_tag="J1138_002310"
     CDS             13186..13542
                     /gene="rpsM"
                     /locus_tag="J1138_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBA8454103.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13559..13948
                     /gene="rpsK"
                     /locus_tag="J1138_002311"
     CDS             13559..13948
                     /gene="rpsK"
                     /locus_tag="J1138_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBA8454104.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13982..14602
                     /gene="rpsD"
                     /locus_tag="J1138_002312"
     CDS             13982..14602
                     /gene="rpsD"
                     /locus_tag="J1138_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBA8454105.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14628..15617
                     /gene="rpoA"
                     /locus_tag="J1138_002313"
     CDS             14628..15617
                     /gene="rpoA"
                     /locus_tag="J1138_002313"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBA8454106.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15658..16041
                     /gene="rplQ"
                     /locus_tag="J1138_002314"
     CDS             15658..16041
                     /gene="rplQ"
                     /locus_tag="J1138_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBA8454107.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            16148..16516
                     /locus_tag="J1138_002315"
     CDS             16148..16516
                     /locus_tag="J1138_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBA8454108.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16527..16952
                     /gene="zntR"
                     /locus_tag="J1138_002316"
     CDS             16527..16952
                     /gene="zntR"
                     /locus_tag="J1138_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBA8454109.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            17008..17226
                     /locus_tag="J1138_002317"
     CDS             17008..17226
                     /locus_tag="J1138_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HBA8454110.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(17223..17636)
                     /gene="mscL"
                     /locus_tag="J1138_002318"
     CDS             complement(17223..17636)
                     /gene="mscL"
                     /locus_tag="J1138_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBA8454111.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17766..19142)
                     /gene="trkA"
                     /locus_tag="J1138_002319"
     CDS             complement(17766..19142)
                     /gene="trkA"
                     /locus_tag="J1138_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBA8454112.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19164..20453)
                     /gene="rsmB"
                     /locus_tag="J1138_002320"
     CDS             complement(19164..20453)
                     /gene="rsmB"
                     /locus_tag="J1138_002320"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBA8454113.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWSHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20505..21452)
                     /gene="fmt"
                     /locus_tag="J1138_002321"
     CDS             complement(20505..21452)
                     /gene="fmt"
                     /locus_tag="J1138_002321"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBA8454114.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(21467..21976)
                     /gene="def"
                     /locus_tag="J1138_002322"
     CDS             complement(21467..21976)
                     /gene="def"
                     /locus_tag="J1138_002322"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBA8454115.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            22106..23230
                     /gene="dprA"
                     /locus_tag="J1138_002323"
     CDS             22106..23230
                     /gene="dprA"
                     /locus_tag="J1138_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBA8454116.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23202..23675
                     /locus_tag="J1138_002324"
     CDS             23202..23675
                     /locus_tag="J1138_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBA8454117.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23704..24246
                     /locus_tag="J1138_002325"
     CDS             23704..24246
                     /locus_tag="J1138_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454118.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            24251..24823
                     /gene="tsaC"
                     /locus_tag="J1138_002326"
     CDS             24251..24823
                     /gene="tsaC"
                     /locus_tag="J1138_002326"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBA8454119.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24828..25646
                     /gene="aroE"
                     /locus_tag="J1138_002327"
     CDS             24828..25646
                     /gene="aroE"
                     /locus_tag="J1138_002327"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBA8454120.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLRGDNTD
                     GIGLLSDLERLSFIRPGLRLLLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMDELEGHEFDLIINATSSGISGDIPAIPPSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25643..25900
                     /locus_tag="J1138_002328"
     CDS             25643..25900
                     /locus_tag="J1138_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBA8454121.1"
                     /translation="MNHAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25876..26430)
                     /locus_tag="J1138_002329"
     CDS             complement(25876..26430)
                     /locus_tag="J1138_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBA8454122.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
BASE COUNT         6837 a         6260 c         6951 g         6651 t
ORIGIN      
        1 ttataaatat aatcaatata ttgatggcag catctttatt atgattcgcc agatggaaag
       61 ggaaaggtag tgtgggcacg atgtatgttc taataaaata gattgggtgg gttgtatatt
      121 gacgttagta taaatagtat taatgccgct tgcatgaatc aagcaaaatt tatttatatt
      181 agtgatatgt atttaattaa taaatacata cattattttt atgattaagg ataatcaata
      241 attaaaacag gaagttctat gtctacgaga agagaagtca ttctttcctg gttgcgtgag
      301 aaacgtcaga cctggcgtct gtgctatttg ttgggtgagg ctggaagtgg aaaaacctgg
      361 ctggcgcagc aactacaaaa agataaacat cgccgtgtga ttactttaag cctcgttgtt
      421 tcctggcaag gtaaggccgc atggatcgtt accgacgata atgcggctga acagggctgc
      481 cgtgacagcg cctggacgcg agatgagatg gcgggtcaat tactgcatgc acttcatcga
      541 actgatagtc gctgccctct tattattatt gaaaatgctc acctgaatca ccgccgcata
      601 cttgatgatc tccaacgcgc gatatctctt attcctgacg gtcaattttt gctgatcggc
      661 agacccgatc gaaaagtcga acgtgatttt aaaaaacagg gcattgaact tgtctcgaca
      721 ggtcgtctga cggagcacga gcttaaggca agcatccttg aaggtcagaa tattgaccag
      781 cccgatctcc ttttaaccgc cagagttctg aaacggatag ctttattatg tcggggagat
      841 cgcagaaagc tggcgcttgc cggcgaaaca attagcttat tacagcaagc cgagcagacc
      901 cgcgttttca ctgcaaaaca atggcgaatg atttaccgcg tattaggtga taaccgcctc
      961 cggaaaatgc agcttgctgt ggtgatgagt ggaaccatta tcgcacttac ctgtggttgg
     1021 ctactgctgt cctctttcac agctccgtta cccgttcctg cgtggctgat acccgtaacg
     1081 ccagtagcta aacaagatat gactaaggat attgcccatg tggtgatgcg cgatagtgag
     1141 gcgctaagcg tgttatacgg cgtatggggg tacgaggtcc cagccgattc ggcctggtgc
     1201 gatcaggcag taagagcggg gctggtatgt aaaagtggga atgcttcttt acaaacattg
     1261 gtggatcaga atttgccctg gatcgcatcg cttaaagtcg gggacaaaaa attgcctgtt
     1321 gtggtcgttc gcgtcggtga tgctaccgtt gatgtactcg tagggcagca aacctggacg
     1381 ctgacacata aatggtttga gttggtatgg acgggtgatt atcttctgtt gtggaaaatg
     1441 tcgccggaag gggagagtac cattacgcgt gatagcagcg aggaagagat tctctggctg
     1501 gaaacgatgt taaaccgcgc attgcatatt tcgacagaat cttcggcaga gtggcgtccc
     1561 ttgttggtag aaaaaataaa gcaatttcaa aaaagccatc acttgaaaac tgacggtgtt
     1621 gttggctttt caacgttagt acatctctgg caggtggcgg gggagagtgc ctacttatat
     1681 cgggatgaag caaatatttc cccagaaaca acagtgaagg ggaaatgaca tgtttgaatt
     1741 ctatatcgcc gcgcgtgagc agaaagaaac gggtcaccct ggtatcttct cccgacaaaa
     1801 acacagcacc ataatatatg tcatttgttt attgctgatt tgcctgtggt ttgcaggaat
     1861 ggtgttggtt ggtgggtatg cccggcagct atgggcactt tggatagtaa aagcagaagt
     1921 cactgtagat gctgaaacgc ccgcttttaa acaatcaaca caacactatt ttttcaaaaa
     1981 acagccactc cccgtcgtcg aatccgtcga ggaggaagat gaaccaggcg tcgcggttga
     2041 gaatgccccc tcttcttctg aagacgaaga aaatactgta gaagaaagcg acgagaaagc
     2101 cggtttaaga gagcgtgtca aaaatgcgct gaacgaactt gagagataac ccaaaggctg
     2161 tttactttac tagcaatacg cttgcgttcg gtggttaagt atgtataatg cgcgggcttg
     2221 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     2281 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     2341 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     2401 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     2461 cgtcgagact gctaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     2521 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     2581 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2641 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2701 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2761 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2821 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2881 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2941 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     3001 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     3061 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     3121 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     3181 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     3241 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     3301 ggcgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     3361 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     3421 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     3481 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     3541 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3601 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3661 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3721 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3781 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgagaacct
     3841 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3901 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3961 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     4021 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     4081 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     4141 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     4201 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     4261 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     4321 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     4381 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     4441 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     4501 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     4561 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4621 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4681 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4741 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4801 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4861 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4921 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4981 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     5041 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     5101 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     5161 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     5221 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     5281 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     5341 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     5401 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     5461 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     5521 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     5581 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5641 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5701 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5761 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5821 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5881 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5941 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     6001 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     6061 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     6121 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     6181 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     6241 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     6301 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     6361 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     6421 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     6481 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     6541 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6601 tggtcgtctg actgcacgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6661 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6721 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6781 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6841 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6901 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6961 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     7021 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     7081 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     7141 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     7201 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     7261 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     7321 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     7381 aatgagccgt ttattttttc tacccatctc cttgaagcgg tgttataatg ccgcgccctc
     7441 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     7501 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     7561 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7621 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7681 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7741 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7801 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7861 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7921 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7981 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     8041 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     8101 caatcttcaa tgcgacaacc ggcaaggctg accgtgtagg ctttagattc gaagacggta
     8161 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     8221 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     8281 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     8341 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatttc
     8401 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     8461 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     8521 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     8581 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8641 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8701 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8761 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8821 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8881 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8941 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     9001 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     9061 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     9121 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     9181 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     9241 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     9301 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     9361 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     9421 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     9481 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     9541 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9601 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9661 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9721 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9781 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9841 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9901 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9961 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
    10021 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
    10081 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
    10141 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
    10201 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
    10261 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
    10321 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
    10381 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
    10441 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
    10501 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
    10561 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10621 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10681 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10741 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10801 ctaaagcgta tggttccacc aacccgatta acgtggttcg tgcaactatc gatggcctgg
    10861 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10921 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10981 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    11041 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    11101 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    11161 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    11221 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    11281 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    11341 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    11401 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    11461 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    11521 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    11581 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11641 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11701 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11761 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11821 gcgtcgatca ttatccagct actgacggtg gttcatccaa cgttggcaga aattaagaaa
    11881 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gctacggtac tctggtgctg
    11941 gcaatattcc agtcgatcgg tattgctact ggtctgccga atatgcctgg tatgcaaggc
    12001 ttggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    12061 accatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    12121 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    12181 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    12241 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    12301 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    12361 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    12421 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    12481 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    12541 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12601 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12661 aaagtaatga cccgcctgac cttggttggt gcgctgtaca ttacctttat ctgcctgatc
    12721 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12781 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12841 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12901 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12961 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    13021 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    13081 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    13141 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    13201 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    13261 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    13321 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    13381 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    13441 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    13501 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    13561 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13621 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13681 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13741 tgcagcacag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13801 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13861 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13921 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13981 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    14041 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    14101 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    14161 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    14221 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    14281 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    14341 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    14401 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    14461 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    14521 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    14581 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14641 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14701 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14761 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14821 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14881 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14941 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    15001 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    15061 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    15121 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    15181 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    15241 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    15301 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    15361 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    15421 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    15481 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    15541 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15601 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15661 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15721 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15781 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15841 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15901 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15961 ttccgtgcag gcgacaatgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    16021 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    16081 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    16141 agtatttatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    16201 aggtgagttt gataacctgg taggtagcgg cgaaccattg atactggatg atgattctca
    16261 tgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgctggtt gcttaccgcc
    16321 agaacttgag cataggaggg aagcaataca gcttctggat attctcaaag gtatccgtca
    16381 tgatgatccg caatatcaag aggttagtcg tcgattgtca ttactggaat tgaagctgcg
    16441 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttgaacaa
    16501 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    16561 aagtaacacc cgacacgatt cgttattacg aaaagcagca gatgatggag catgaagtac
    16621 gcaccgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16681 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16741 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16801 aagtcgaagc tcgtatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaatg
    16861 atacctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16921 aaggggcgag tggcgttaag agtggctgtt gattttttgc actggcagga ttacactcgc
    16981 gccgttaaat aaccaattgg agtttttatg agccgatatc agcatactaa agggcagata
    17041 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    17101 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    17161 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcagacgct
    17221 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    17281 tctttagttg gtgcaggtgt tgctgctgct ggttcttctt ttttccgatt cagtttgttg
    17341 attagcttaa tcgccataaa gatggcaaag gccactatca gaaaatcaaa gacgttttga
    17401 atgaagacac cgtaatgcat cacaacagca gggatatccc cttgggcctc gcgtagcgtg
    17461 acagcaaact gtttaaagtc gatcccgcca attaataagc ccagaggtgg catgatgata
    17521 tcggcaacca gtgaagatac aatcttcccg aatgccgctc cgataatgac acccactgcc
    17581 aaatccacca cgttcccgcg catcgcaaat tcgcgaaatt ctttaataat gctcatgtta
    17641 ttctccctat gaagccgaca accataagtc taacaaatgt taagccattt tcctgccagg
    17701 aatggattta attaatcctt aataatcaaa gggaaataat aaggcgtcat tagacgcctt
    17761 attaattaca agaagaaagg acttggctgg aagagtcttt cgacgtcggt aataaacttt
    17821 ttatctgtga ggaacataat tacgtgatcg ccttgctcaa tgcgcagatt gtcattggca
    17881 atcatcacat cgtttccacg taccaccgct ccaataatcg ttcctggcgg tagcttgatt
    17941 tcgtcaatga ctctgccgac aacgcgtgag gtgctttcat caccgtgagc aacggcttca
    18001 atagcttctg ctacgccgcg gcgtaatgag gaaacaccaa caatatctgc ttttcgcaca
    18061 tggctaagca acgcagaaat agttgcttgt tgtggtgaaa tcgcaatatc gatcacgctc
    18121 ccctgaacca gatccacata agcgcgacgt tggatcaata ccatcacctt tttcgcaccc
    18181 atacgtttgg caagcatggc ggacatgata ttggcctcgt catcgttggt gacggcaata
    18241 aacagatcaa cttgatcgat atgttcttcg gccagcagtt cttgatccga cgcatcacca
    18301 aaaaagacga tcgtattctg caacttttcc gccagttcgg cagcgcgctg ctgattacgt
    18361 tcgatgagtt taacgctgta atctttttcc agacgacgcg ccagccctgc accgatatta
    18421 ccgccaccaa ccagcatgat ccgcttatac ggtttttcca gtcgctgtaa ttcactcatc
    18481 accgcacgga tatgctgtga agcggcaata aagaacactt catcaccagc ttcaacaatg
    18541 gtcgaacctt gcggacgaat ggggcgatcg tggcggaaaa ttgctgccac acgagtatcg
    18601 atatgtggca tatgttcgcg catggtcgaa agtgcattac caatcagcgg gccgccataa
    18661 taggctttaa ccacagccag gctgacttta ccctcagcga agttcaccac ctgcaatgcg
    18721 ccgggatact caatcagtcg gtaaatatta tcgataacca actgctctgg tgcgatcaga
    18781 tgatcaatcg gaacagcatc tgaatgaaat agcttatcgg catcgcgcac gtagtctggt
    18841 gagcggatac gagcgatgcg attaggggtg ttgaaaagtg agtaggctac ctggcaggca
    18901 accatattgg tttcatctga actggttaca gcaaccagca tatcggcgtc gtcggcacct
    18961 gcctcccgca atacgcgtgg atgagagcca tgcccctgca cgactcgcag gtcaaattta
    19021 tcctgtaagg tccgcagacg ctcaccgttg gtatcgacaa cagtaatatc gttgttctcg
    19081 ccaaccaggt tttccgccag tgtgccgcca acctggccgg cacccagaat gataattttc
    19141 atcagtcgcg acccgttatc tcatcacttt ttgattagct tagcgtaaaa gaagccgtcg
    19201 ccctcttcgg caccgggcaa attttgttta cccggttgct ctggtgttcc tgtttcgcaa
    19261 agttcggcat cagcggtacg ttgcaaaaag gctttaatct gcaggctatt ctcttccggt
    19321 aacaccgaac aggtggcata gaccagggtt cctccggatt ttaaatgcga ccagatggcg
    19381 tcgagaattt cagactgcaa ttgcgcgagt tccgggatat cgcgatcacg acgtaaccat
    19441 ttaatatccg gatggcgacg aatcaccccc gttgctgaac aaggcgcatc taataaaatg
    19501 cgatcgaact gttgctcgcc acaccattgg gaagggtagc ggccatcgcc ttgtttcacg
    19561 gtcgccttca taccaaggcg ttttaaattg tcgtaaacgc gagagaggcg ctgctcatca
    19621 atatcgaccg ccagaacttg cgcctctgga gcaacctcaa gaatatgcgt tgttttaccg
    19681 cctggggcgg cgcaaagatc cagaatgtgt tcaccatttt gtggagcaag ccaggtcatg
    19741 caaccttgtg ctgaagcatc ctgaacggta acccatcctt cttcaaaacc gggtagcgca
    19801 tgaacaggtg caggtgtttc cagctgtaca gcatcagggt aatccgcatg cgggaaacct
    19861 ttcattcctg cttcatccag caatgcaagc cagctgtcgc gggaatgatg cgtacgatta
    19921 acgcgcagcc acattggcgg acgctggtta ttggcttcga cgatggattg ccactgctcg
    19981 ggatacgcct tttgcagacg cttcagcaac caggaaggat gcagataacg tgcatcactg
    20041 gcattaaact cggctaataa ctcatcttgc tgacgctgga actgacgtaa tacaccgtta
    20101 atcaatccct taagttgcgg gcgcttaatt gcaactgcgc cttcaaccgt ttcagccagc
    20161 gcggcatgag gtggaatgcg ggtataaagc agttgataca aaccaaccat aatcaggtaa
    20221 tgaacagtac gctgtttgcc tgtcatcgga cgggccatta gcttattaat cagccagtct
    20281 aactgtgaga gcgtacgcag tacgccaaag cataactcct ggagaagagc tttatcttta
    20341 tcggaaactt tttgctggag cggtggcaga atgttgctca gtgattgccc ttgctcaacg
    20401 acttgttcaa tggcctgggc cgccatgcta cgtaaattac gttgtttttt cataagtata
    20461 aatataaaga tgcccggtaa gaccgggctt agaagagtgg actatcaggc cagacggttg
    20521 cccggtacaa accattcccg acgagaattc aggaggtctt gcacgctcat cgctttctta
    20581 cctgcaggtt gcattgagag cagattcagg atgccatcac ctgtcgctac ctgaatgcct
    20641 tgtttattgg cttcaaggat tgttcctggc gcagcctttg ttgtcgtatc gatgacagat
    20701 gctttccaga ctttaaccgg ctgcccttca atttccagcc agctcattgg ccatggattg
    20761 aaagcgcgaa tgcaacgttc aagctgtgct gccgaaagtg accagtcaat acgcgcttct
    20821 tctttactca acttttcggc gtaagtgaca agagtttcgt cctgaacttc tggtttcgcc
    20881 gtgccgtctg ccagttgttt caacgtagta ataagccctt gtgggccaag ctctgccagt
    20941 ttgtcgtaca gcgtaccact ggtatcttct gcagtaatcg ggcaggagag cttatagagc
    21001 atgtcaccgg tgtctaaacc aacatccatt tgcataatgg tcacaccagt ttctgcatca
    21061 cccgcccata gtgagcgttg gattggtgca gcaccgcgcc agcgtggcag cagtgaacca
    21121 tgaacgttga tacagccaag acgcggcatc tccagcacag ctttaggcag aattaaacca
    21181 taggcgacga cgaccataac atcagcctgc agatcggcga ccagttgctg gttttcttgt
    21241 ggacgcaggg aaacaggttg aaaaacgggc agacctttgt cctcagccag aactttaacc
    21301 gggctgggca tcagtttttt accgcgtcct gccggtcggt ctggctgggt aaacacgcca
    21361 acgatgttat gaccagaaga caacagcgcg tcgagatgac gcgctgcaaa gtcaggtgta
    21421 cccgcaaaaa taatacgtag tgattctgac acgttaattc ttatccttaa gcccgggctt
    21481 tcagacgatc cagtttttca actttctgac gaatacgttg ttgtttcagc ggtgacagat
    21541 aatccataaa cagtttgccg accaggtgat ccatctcatg ctgaatacag atggctaaca
    21601 gaccgtctgc ttccagttca aatggtttac cgtcgcggtc cagggcgcga attttaactt
    21661 tctctgcgcg tggcactaaa gcacgttgtt cagggatcga caggcaacct tcttcaatgc
    21721 ctgtttcgcc gcttttttct aaaagctccg gattgattaa caccagccgt tcgtcacggt
    21781 tttccgaaac atcaattaca atgatacgtt gatggatatc aacctgggtt gccgccaggc
    21841 caataccttc ttctgcgtac atcgtctcga acatatcatc gacgatacgc tgaatttctg
    21901 cattcacttc ttctaccggt ttagcaactt tgcgaagccg ctcgtccgga atatgtaaca
    21961 cttgcaaaac tgacataaat ctccagagat gtgttcagga gttagaaaga ttatttcttc
    22021 tattctagac aaatccccct ctgattgaca gcatcactga ccaatcgcaa agattgctaa
    22081 ggctgcttat ggcagggaga taaggatggt cgatacagaa atttggctgc gtttaatgag
    22141 catcagcagc ttgtatggcg atgatatggt ccgtatagct cactggctgg caaaacagtc
    22201 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
    22261 tctttcattt ccgcgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
    22321 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttctggcga cgacagatta
    22381 ccccggcgca ctgtttgttg aaggagaact gcacgcgttg cattcatttc agcttgccgt
    22441 agtggggagt cgggcgcatt catggtatgg cgagcgatgg ggacgattat tttgcgaaac
    22501 tctggcgacg cgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggc
    22561 gcataaagcg gccttacagg taaatggcgt tagcattgct gtattgggga atggacttaa
    22621 taccattcat ccccgccgcc atgctccact ggctgccagt ctacttgagc aaggtggtgc
    22681 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
    22741 tcgcattatc agtggcctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
    22801 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ctttgccagg
    22861 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
    22921 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
    22981 cgcccctgaa aattcatttt attcaccaga tcaggaagac gtggcattgc catttcctga
    23041 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
    23101 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
    23161 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
    23221 atttgtttga aacctatatt cacacagaag ctgagttgcg tgtggatcaa gacaaacttg
    23281 aacaggatct taccgacgca ggatttgatc gagaagatat ctacaatgcc ctgctttggc
    23341 tggaaaagct tgcggattat caggaaggac tggcagaacc gatgcaactg gcctctgatc
    23401 cactctccat gcgtatttat acccctgaag agtgtgaaag actggatgcc agctgtcgtg
    23461 gttttctgct tttccttgag caaattcagg tgcttaacct tgaaacccgt gaaatggtga
    23521 tagagcgagt gctcgcgctg gataccgctg agttcgacct ggaagacctg aaatgggtaa
    23581 tcctgatggt gttgttcaat attccgggct gcgaaaatgc gtaccagcaa atggaagaat
    23641 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagta
    23701 gttatggcga aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
    23761 gctgaactag ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
    23821 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
    23881 gaggggcagg tttgccctgc atgtggcgca aatctggtat tacgccaggg acgctttggt
    23941 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
    24001 gacgaaacag caattacatg cccccaatgc cggacgggcc atctggttca gcgccgctcc
    24061 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
    24121 ttcaaaccca tagctggaga atgccctgag tgccattatc cgctactcat cgaaaagaaa
    24181 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc gatttcggcg
    24241 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
    24301 caatgaagaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
    24361 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cggcgtccgg ttgataaggg
    24421 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgct
    24481 gactgacgcg cagcgtgaaa ccattttttc ccgctggcca ggtcctgtca cctttgtctt
    24541 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
    24601 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
    24661 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
    24721 atttggcgct gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
    24781 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
    24841 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
    24901 agcaactgaa tattgaacat ccctatgggc gtgtgttggc acccatcaat gatttcatca
    24961 acacactgaa cactttcttt agtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
    25021 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgctg
    25081 ttaataccct caagcggtta gaagatggac gcttgcgggg tgacaatacc gatggtatag
    25141 gtttgttaag cgatctggaa cgtctgtctt ttatccgtcc tggtttacgt cttctgctca
    25201 tcggcgctgg tggcgcatct cgcggcgtac tactgccact cctttccctg gactgtgcgg
    25261 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
    25321 ctggcagtat tcacgcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
    25381 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgcca tcgctcattc
    25441 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
    25501 ggtgtgagca gcgaggctca aagcgtactg ctgatggttt aggaatgctg gtggcacaag
    25561 cggctcatgc cttccttctt tggcatggcg ttctgcctga tgtagaaccg gtcatcaaac
    25621 tattgcaaca ggagttgtca gcgtgaatca tgccatccag tttccggata gggaagagtg
    25681 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
    25741 tgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
    25801 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
    25861 acaaagtgaa gatgatcaag gttgggtgtg gttaccctga tccatatatt cgtccttcca
    25921 tttcacgtag ttatttgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
    25981 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
    26041 gaccaggcta cctgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
    26101 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
    26161 accaacagtg acatcttcgc caatggttaa tgggttgcca gctgggttgt acgaggattt
    26221 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
    26281 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
    26341 gtcaccaatc acgacgctgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
    26401 atcgcggtat gggcgtaaaa catcagacat actcacctca acaataaatg atttactaat
    26461 gactttgggt acattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
    26521 gtgtgcgaat gcagcaaaaa gggtgaaaaa acaacaaaca gaaaaaaaga tcaaaaaagt
    26581 acttgtgcaa aaaattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
    26641 cttcacaaga tagtcgtaac aacaaagaga aaaatcctga aattcagggt tgactctga
//
LOCUS       DADTFG010000061        26278 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.061, whole genome shotgun sequence.
ACCESSION   DADTFG010000061 DADTFG010000000
VERSION     DADTFG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.061"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(34..652)
                     /locus_tag="J1138_002330"
                     /pseudo
     CDS             complement(34..652)
                     /locus_tag="J1138_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            919..2414
                     /gene="ansP"
                     /locus_tag="J1138_002331"
                     /pseudo
     CDS             919..2414
                     /gene="ansP"
                     /locus_tag="J1138_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
     gene            complement(2527..3588)
                     /locus_tag="J1138_002332"
     CDS             complement(2527..3588)
                     /locus_tag="J1138_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBA8454123.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3830..5932
                     /locus_tag="J1138_002333"
     CDS             3830..5932
                     /locus_tag="J1138_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA8454124.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRLTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLILNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            complement(6009..6164)
                     /locus_tag="J1138_002334"
                     /pseudo
     CDS             complement(6009..6164)
                     /locus_tag="J1138_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(6164..6232)
                     /locus_tag="J1138_002335"
                     /pseudo
     CDS             complement(6164..6232)
                     /locus_tag="J1138_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase yncb"
     gene            6413..6931
                     /gene="mddA"
                     /locus_tag="J1138_002336"
     CDS             6413..6931
                     /gene="mddA"
                     /locus_tag="J1138_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBA8454125.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            6928..7377
                     /locus_tag="J1138_002337"
     CDS             6928..7377
                     /locus_tag="J1138_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA8454126.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(7378..7611)
                     /locus_tag="J1138_002338"
     CDS             complement(7378..7611)
                     /locus_tag="J1138_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBA8454127.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(7697..7870)
                     /locus_tag="J1138_002339"
     CDS             complement(7697..7870)
                     /locus_tag="J1138_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HBA8454128.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRLLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            8065..8160
                     /gene="yncL"
                     /locus_tag="J1138_002340"
     CDS             8065..8160
                     /gene="yncL"
                     /locus_tag="J1138_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBA8454129.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(8257..9681)
                     /gene="patD"
                     /locus_tag="J1138_002341"
     CDS             complement(8257..9681)
                     /gene="patD"
                     /locus_tag="J1138_002341"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBA8454130.1"
                     /translation="MQHKLLINGELVRGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(9703..10497)
                     /locus_tag="J1138_002342"
     CDS             complement(9703..10497)
                     /locus_tag="J1138_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8454131.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(10487..11428)
                     /locus_tag="J1138_002343"
     CDS             complement(10487..11428)
                     /locus_tag="J1138_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8454132.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(11429..12442)
                     /locus_tag="J1138_002344"
     CDS             complement(11429..12442)
                     /locus_tag="J1138_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8454133.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            complement(12460..13605)
                     /locus_tag="J1138_002345"
     CDS             complement(12460..13605)
                     /locus_tag="J1138_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA8454134.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHNLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(13850..15256)
                     /locus_tag="J1138_002346"
     CDS             complement(13850..15256)
                     /locus_tag="J1138_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA8454135.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNVELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQHAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(15335..15571)
                     /gene="hicB"
                     /locus_tag="J1138_002347"
                     /pseudo
     CDS             complement(15335..15571)
                     /gene="hicB"
                     /locus_tag="J1138_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            15750..15980
                     /locus_tag="J1138_002348"
     CDS             15750..15980
                     /locus_tag="J1138_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HBA8454136.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(16072..18033)
                     /gene="rlhA"
                     /locus_tag="J1138_002349"
     CDS             complement(16072..18033)
                     /gene="rlhA"
                     /locus_tag="J1138_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBA8454137.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(18106..18642)
                     /locus_tag="J1138_002350"
     CDS             complement(18106..18642)
                     /locus_tag="J1138_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8454138.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSSTPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            18695..19906
                     /gene="ydcO"
                     /locus_tag="J1138_002351"
     CDS             18695..19906
                     /gene="ydcO"
                     /locus_tag="J1138_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBA8454139.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNETIGVFIVTNVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDIVFKPVLPTYIPPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            complement(19941..20609)
                     /locus_tag="J1138_002352"
     CDS             complement(19941..20609)
                     /locus_tag="J1138_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBA8454140.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(20912..21505)
                     /gene="tehB"
                     /locus_tag="J1138_002353"
     CDS             complement(20912..21505)
                     /gene="tehB"
                     /locus_tag="J1138_002353"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBA8454141.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(21502..22494)
                     /gene="tehA"
                     /locus_tag="J1138_002354"
     CDS             complement(21502..22494)
                     /gene="tehA"
                     /locus_tag="J1138_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBA8454142.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYELLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            22618..23598
                     /locus_tag="J1138_002355"
     CDS             22618..23598
                     /locus_tag="J1138_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBA8454143.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTPLAGLL"
     gene            complement(23590..24129)
                     /gene="rimL"
                     /locus_tag="J1138_002356"
     CDS             complement(23590..24129)
                     /gene="rimL"
                     /locus_tag="J1138_002356"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBA8454144.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(24192..24416)
                     /locus_tag="J1138_002357"
     CDS             complement(24192..24416)
                     /locus_tag="J1138_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBA8454145.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            complement(24556..26211)
                     /locus_tag="J1138_002358"
     CDS             complement(24556..26211)
                     /locus_tag="J1138_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HBA8454146.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
BASE COUNT         6491 a         6671 c         6471 g         6645 t
ORIGIN      
        1 gtgattcatt aacattaacc tccggcatta ttgttaaata atgacattcc tttttaatac
       61 ttcttgtaat ttcggtatct gacatacagc atcaacaatc gcattaatgt tcggggcatt
      121 atcctgaaac cattcgtgtc ccggtcccca tgtgcgcatg gtgcagagat aacagtccac
      181 cagcgttagt tgctctccaa acacatacgg ttcagcagtc aattgcgaat tcagccagat
      241 ataaagcgat ttgcgatatt caattacact cttctttaac tgttcaggtg cgtcagatgc
      301 ccaacgctta gggtaatcgg cgaaagtgaa cgttggatag acattagcaa ccagccagac
      361 caatagccgt tgaaactgtt gacgttcggc gcgcccaaga ggtggcgcaa ggtccgggcg
      421 acgatcgaga accatcaatg caatcgccgc tgtctccgtc ataatctcgt tatttttaca
      481 gcgccagtgt cggaacctga cacaacggat tcagggtttt taataaatcg cgtgaagttc
      541 cctcgtggtc aaaaccactg acatcaacga actgataagg aatatcagcc agtgttagca
      601 tcagctcact gattgtagag ccccagccgg gtacgccata caccttaatc atatgcccct
      661 cacaatcttt taaagattaa gtgtaggaca gcacacaggt tatccagcaa aaggatttat
      721 cattttttcg tcagttgtat aaatcccctt caggattgta aggggatgtt ttgcatcgtt
      781 tatcgataac gggtaggatg ctgcgccgat agttttatac ccataaagaa taatggtgat
      841 aactatcatc gccaggatga ataaacattg ttcatggcaa cttatatgac tttttcatta
      901 aagcaatcag ggagagcaat gagtaaacac aacaccgaca cttcagatca acacgccgcg
      961 aaacgtcgct ggcttaatgc ccacgaagag gggtatcaca aagcgatggg caatcgccag
     1021 gtacagatga tcgccattgg cggcgcgatt ggtacgggtt tgtttttagg cgcgggggcg
     1081 cgactgcaaa tggcggggcc cgcactggca ctggtctatt tgatctgcgg cctgttttcg
     1141 ttttttattc tgcgtgcatt gggtgagctg gtgttacacc gcccttccag tggcagtttt
     1201 gtctcttatg cccgtgagtt tttgggtgag aaggccgctt atgttgctgg ctggatgtac
     1261 ttcatcaact gggcgatgac ggggattgtt gatattaccg ccgtcgctct gtatatgcat
     1321 tactggggcg cgtttggcgg cgtgccgcag tgggtctttg cgctcgctgc actgaccatc
     1381 gttggcacca tgaatatgat cggtgtgaaa tggtttgcgg agatggagtt ctggtttgcg
     1441 cttattaaag tgctcgccat tgtaaccttt ttggtcgtgg gtacagtgtt cctcggtagt
     1501 ggtcagccgc tggatggcaa cgccactggc tttcatttaa ttaccgataa cggcggcttt
     1561 ttcccccacg gtttgctgcc tgcgctggtg ttgattcagg gcgtagtgtt tgcttttgcc
     1621 tccattgaaa tggtgggtac agcggccgga gaatgtaaag atccgcagac catggtgcct
     1681 aaagcgatta acagcgtgat ttggcgtatt ggcctgtttt acgtcggctc cgtggtgttg
     1741 ctggttatgt tattgccgtg gagcgcgtat caggcggggc aaagtccgtt cgtgacgttt
     1801 ttctctaaac tgggtgtgcc gtacatcggc agcattatga acattgtggt gctgaccgct
     1861 gccctctcca gcctgaattc aggtctgtac tgcaccgggc gtattctgcg ctcgatggcg
     1921 atgggcggtt ccgcaccgag ttttatggcg aaaatgagtc gtcagcatgt gccgtatgcc
     1981 gggattctgg cgacactggt tgtgtatgtc gtcggcgtat tcctcaacta tctggtgccg
     2041 tcacgcgtat ttgagattgt gttgaacttc gcgtcgctgg gaatcatcgc ttcatgggcg
     2101 tttatcatcg tgtgccagat gcgcctgcgt aaagcaatta aagaaggcaa agcagcggat
     2161 gtcagtttta aactgcctgg cgcgcccttc acttcctggc tgacattact gtttttactg
     2221 agtgtcctgg tgctgatggc gttcgattac ccgaacggga cttataccat cgcggcgctg
     2281 ccgattatcg gtattctgct ggttataggt ttggtgtgcg caaacgcgtt gctgaaattc
     2341 acagcactgc gccagtcgtc gaggaagatg aagaaaaaca ggaaattgtg tttaagcctg
     2401 aaacatcgag ttaattgcaa aattgcccgg ctacgtgccg ggcaaattca gactactgac
     2461 gaaggtgccg gatgcggtgt gataaaagtc aggcaattct ttccgttttc accctcgcca
     2521 tatctattac agcgcaatac gaatcacatc gtccggctgg gtagcttcct gctgtttagt
     2581 ggatttttgt ttcacactga catacagcgt tttgccatcg gcagacagcg ccaggctgtt
     2641 cggatgagtc ggcgtatcga acgttttcac cactttatag cttttcgcgt caatcacact
     2701 gactttacct gcctgacgat gcgtcacgta ggcttcatta cgcgctgggt taaacagcac
     2761 agccagtgat tccggtgccg caaccttcgc cagaatattg ccattacggg tatcgaccac
     2821 taacacttct gccgctttag aatcggtgat aaatgcacgc tgattggtgg tatcaaggct
     2881 gatgttgata aagaagtgct ctttgccgtc atccagcagc tttttacggc tgaggatttt
     2941 attgtcggcg gtgtcgatgg taatcaattc gccgtcagcg ttagtggtgt aaagacgttt
     3001 gcctttgcta tccagcgcca gaccggtact cattttaccg gtgttctgga tggcggtttt
     3061 cagtttgata ttctcgccat caacgaccca aatcacgctc tctttaccaa taccactgat
     3121 gtaaacggtg ttcgtggcat catcagctac cagctcacgt ggttgcaacg ggcgtacctc
     3181 ttccgtgcgc ttacgatcat ccagcaccag acgacctttc acttcgcccg ttttggcatc
     3241 tatcgccgtg accgcactat ttacggtgtt accaaaccac aacgtctgag tcgtgttatt
     3301 gatggtggca ccaaacggct tgagatcgtt atggatcgcc tgcgtcactt ccagagtaac
     3361 cggatcaaga cgataaacca cgccgccttt atccagtttg cggctttgcg aagtggcgag
     3421 ccacagcgcg ttttcttgct ggctataagc catttcgtag gcacctttac ctaccgcttt
     3481 acgcagcatt tcttctgcgg cctgcgtact gaatgatgaa gcaacaagca atgaacctaa
     3541 cagtaatgaa ccacgcaggc gcgatgaaaa cagatgacgt aaatgcatga cgactccctt
     3601 tgatgaaccg atatttttgc tgcttcacaa tgcttcgcta agcgtaatca tcgttacgcc
     3661 cttgttataa atgggaatgg taatcattat tttctcaaat gtggagcatt tcttaccgtt
     3721 acgactacaa ttttctcaat tacttaacgt attgtgccgt ttcgttttcc ataaatttac
     3781 aatttcatta ccatgacggt gttttcccat ccttaacctg gcttttttca tgaagatttt
     3841 ttccgtccga cagaccgttt tgcccgcact gcttgtcctt tcccccgttg tttttgccgc
     3901 tgatgaacag accatgattg tcagtgccgc accgcaggtg gtttcagaac tggatacccc
     3961 agcagccgtg agcgtggtgg atggcgagga gatgcgcctg gcaacaccgc gcattaactt
     4021 gtctgaatca ctgaccggag tgcctggttt acaggtacaa aaccggcaga actatgcgca
     4081 agatttacag ctgtcgattc gcggatttgg ctcccgctcc acttacggaa ttcgcggtat
     4141 tcgcctgtat gtggacggta ttcccgctac catgcccgac ggacaagggc aaacatctaa
     4201 catcgattta agcagtgtgc aaaatgtgga agtgctgcgt ggccccttct ctgctctgta
     4261 tggcaacgcg tctggcggtg taatgaatgt caccacccag accggacaac agccaccaac
     4321 cattgaagcc agtagttact acggcagttt tggcagctgg cgctatgggc tgaaagctac
     4381 gggcgcaacg ggcgacggca cacaacccgg cgatgtcgat tacaccgtct caaccacacg
     4441 tttgacgacc cacggctatc gtgaccatag cggcgcacag aaaaatttag ccaatgccaa
     4501 actgggcgta cgcattgatg acgccagcaa attaagcctg attctcaata gtgtggatat
     4561 caaagcagat gacccaggtg ggctaaccga agcagaatgg aaggcgaatc cacaacaagc
     4621 gccccgtgca gaacagtacg acacgcgaaa aaccatcaag caaactcagg ctgggttgcg
     4681 ctatgaacgt agcctgagcg cgcaagatga tatgagtgta atgatgtatg ccggagagcg
     4741 agaaacgacc cagtaccagt caatccccat ggccccgcaa cttaacccgt cacatgcggg
     4801 cggcgtgatt accctgcaac gccattacca ggggatagac agccgctgga cacaccgtgg
     4861 tgaattgggc gttccagtca cgttcacaac cggcctgaac tacgaaaaca tgagtgaaaa
     4921 ccgcaagggt tacaataact tccgcctgaa tcgcggcgtg ccggaatacg ggcaaaaagg
     4981 tgagttgcgc cgcgacgaac gcaatttgat gtggaacgtc gatccctatt tacaaaccca
     5041 gtggcaactg accgacaaac tttcgctgga tgctggcgtg cgctacagct ctgtgtggtt
     5101 tgattccaat gatcattacg ttactccggg taacggcgac gacagcggtg atgccagtta
     5161 tcacaaatgg ctaccagccg gatcgttaaa atatgcaatg accgatgcct ggaatgtcta
     5221 tctggcagcc gggcgcggtt ttgaaacgcc gacgattaat gagctgtctt atcgcgctga
     5281 tgggcaaagc ggtatgaact ttggtttaaa accctccacc aacgatacaa ttgagatcgg
     5341 cagtaaaacg cgtattggtg atgggctgct tagtctcgca ttgttccaga ccgacaccga
     5401 tgatgaaatt gttgtcgata gcagtagcgg tgggcgtacg acttacaaaa atgccggaaa
     5461 gacccgtcgt caaggcgctg aactggcatg ggatcaacgt ttcgcgggag attttcgcgt
     5521 aaaagcgtcc tggacctggc ttgatgcgac ctatcgcagc aatgtttgca atgaacagga
     5581 ttgtaacggt aaccggatgc cagggatcgc ccgtaatatg ggctttgcgt cgataggtta
     5641 tataccggaa gatggttggt atgcaggcac ggaagcgcgt tatatgggcg atattatggc
     5701 agatgatgaa aatacggcca aagcgccttc ttatacactc gtcggcttat tcaccgggta
     5761 taaatacaat taccacaatt taaccgtgga tttatttggt cgtgtcgata atttattcga
     5821 taaagaatac gttggttctg tcattgtcaa tgagtcaaac gggcgatatt acgaacctgc
     5881 gcccggacga aattatggtg tcggcataaa tattgcgtgg cgatttgagt aattatcttt
     5941 aagcccggta tttgcagggc gtattttatt tatcggctgt attgctggca caacatagca
     6001 acgttttttt gcatagtgag gttttcgaca gccagagcaa ctgccattaa ttccagttcg
     6061 tagcggatcc gattgatttc atttaattga cgttttccca cttccggaac tgtaaatgct
     6121 tgcgctggtg cgacaaagag cacatttacc gaaaccagac gtaaggaacc ggcgcgccat
     6181 gcggacgcga ggacagaacc caacatcggt tacgctgcgt ttgttgcccc ataatgctct
     6241 cctgtgcttt tggcatgaat ccatcaatag tagcaggctc acaggattgg ttttgtttgc
     6301 agccgtcaca tcccctctgc aacacgcaat aatttcatag aggaatccgt tgcttcgggc
     6361 tgttttcgat agtattgata acatcatcat cattatttta tcgaggccag acatgtccat
     6421 ccgttttgcc cgaaaagccg actgtgctgc aattgcggaa atttataacc acgccgtgtt
     6481 gtatacggcg gctatctgga atgaccaaac ggtggatgcg gataaccgca ttgcctggtt
     6541 tgaagcgcgg accatagcag gttatccggt gctggtgagt gaagaagatg gcgtagtgac
     6601 gggatatgcc tcgtttggcg actggcgtag ttttgatggt tttcgccata ccgtggaaca
     6661 ttcggtttat gtccatcccg atcatcaggg gaaaggtctg gggcgtaaat tgttaagccg
     6721 attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca
     6781 aaatcaggcc tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg cgcaaatgcc
     6841 gcaggtaggc actaaatttg ggcgctggct ggatctgaca tttatgcagt tgcaactcga
     6901 cgagcgcact gaaccggacg cgattggatg aatcagtcac ttacccttgc ctttttgatt
     6961 gcagccggga ttggtctggt agtccagaac acgctgatgg tgcgcatcac ccagacctcc
     7021 tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct ctttgtttct
     7081 attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag cgtgcgctgg
     7141 tggacactta tacctggctt gctgggatcg ttttttgtct tcgccagtat cagcggatat
     7201 cagaatgtgg gagcggcgac aactattgca gtactggtcg ccagccagct gattggcggt
     7261 ctggtgctgg atatcttccg cagccatggc gtaccacttc gggcgttgat cggcccaatt
     7321 tgtggcgcaa ttttacttgt ggttggcgcc tggctggtgg ccagacgctc attttaatca
     7381 gaggatggta ccgcctttgg ttaactgttc gtggcgcgct tccatatctt ctttatgttt
     7441 gcgaccatga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg
     7501 acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg caatgacgct
     7561 ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca tactttctcc
     7621 ttagccactt gtgaaattta tcgattcatc caaatgatat aagtctgcgt ggtgccgtca
     7681 agccagtgcg taagatttaa aacagtgaaa ataacagcgc caccgggaaa ctcatcggcc
     7741 atgttgcccc caccagaaag gcgcttaaga gacggatgcg tttcttatcg tgagaaagaa
     7801 accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc atgtgttgat
     7861 agagagacat gtacgggcgt ccagccagat gaataaaacg cgcgcaaata tgcgtcattt
     7921 tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc
     7981 cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact atacacatag
     8041 gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt actgataaat
     8101 gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg gtttatttga
     8161 tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc agtgctctga cagtacgcaa
     8221 gatcatctca tactcttacg atctgcaata ttatccttaa tgtttaacca tgacgtggcg
     8281 gacgacggtg taatcctcca gcccataaag tgacatatcc ttgccgtaac cagaaagttt
     8341 ctgcccaccg tgcggcattt cacttaccag catgaaatgg gtattgaccc aggtacaacc
     8401 atattgcagc cgcgcgctga cgcgatgcgc cctgcccaca tctttcgtcc ataccgaaga
     8461 tgcaagtccg tactgactgt cattcgccca gttcaccacc tgttcttcgt tgtcgaagag
     8521 cgtaacactc actaccggac caaatacctc tttttgcacg atggcatcgt cctgtaatgc
     8581 gccagccagc agcgtcggcg catagtaata gccattaccc ttgcgctttt caccgccagt
     8641 gatcactttg atgtgccctg tcgctttcgc ctcttctacc gccttgctga cgcgttcgag
     8701 atgcgccagc gagcttaaag gtccaagctc cgtagactcg tcatccggcg aacccgattt
     8761 taacgttgcc accgcagcac cgagtttttc caccagcgta tcgtaaatgc ctttttgcgc
     8821 gtagatccga caagccgcag tacaatcctg tccggcattg taatagccaa aagtacgtac
     8881 accttcgacc actgcttcaa tatccgcatc atcaaaaaca atcactggcg ctttgccacc
     8941 aagttccata tgagtacgct taatggacgg cgcggtatgg ctgatgatgt gttccccagt
     9001 ggcgatagaa cccgtcagcg acaccatccg cactttggga tgtccggtca gcggatctcc
     9061 caccgttttg cctctgccaa acagcacgtt aatcacgcca gccgggaaga tatctttcgc
     9121 cagctctgcc aacttcaacg cggtcagcgg ggtaatttct gatggtttaa gcactacgca
     9181 gttccctgcc gccagcgccg gagccagttt ccacgcagcc atcatcagcg gataattcca
     9241 cggtgcgata gaagccacta cccccaacgg atcgcgacgg atcatcgaag tatgaccttc
     9301 aagatattca cctgccgcca gaccattcag gcagcgcgcc gcaccggcga aaaagcgaaa
     9361 gacatcgaca atcgccggaa tttcatcatt gaacgcgcta tgcagcggtt tgccacaatt
     9421 acgggactcc agttcggcaa aaacctgacc attttcttca ataacatcag ccagtttcag
     9481 caggcattcc gcacgcgctt ttggcgtggt ttgcccccat tcggcaaatg ctgcatctgc
     9541 cgcgcgaaca gcagcattga cctgctctgc ggatgcctcg gcaatctcca gtaaaacgtc
     9601 ccccgttgcc ggattataga caggctgttt ttccccttcg ccgcgaacca gttctccgtt
     9661 aatcagtaac ttatgttgca tagcgtattc ctgtatcagt ggttattgac cattgtcgcc
     9721 ttcgcgggtt agccaccagg cccccaggat cggcaaggtt gttaccaaca taaccagcag
     9781 tgccaccacg ttggttaccg ggacatcacg cggtcgccca agctgattga gcaaccacaa
     9841 cggtaacgtt cgttcatgac ctgccgtaaa tgtcgtcacg atgatttcat cgaacgacaa
     9901 ggcaaacgcc agcattcctc cagccagtaa cgccgaactg agattcggca acactacgta
     9961 gcggaaggtt tgccagccat tggccccaag atccattgac gcctcaacca gactccacga
    10021 ggtgcggcga aaacgggcga tgacattgtt aaacaccacc actacgcaaa aagtcgcatg
    10081 accgaccacg atggtgaaaa atcccggctc cagattgatg gttttaaagg cggttaatag
    10141 cgccagacca gtgacaatgc ccggcagcgc aatgggcagc aacagtaaca gcgaaatggc
    10201 gtttttgccg aaaaagtctc gtcgccacag cgcggcagct gccagcgtcc ctaacaccag
    10261 cgcgattaat gtcgccagcg ccgccacttt aagtgacagt gtcacggcat caagaatatc
    10321 actacgctgt gctgccacgc taaaccagcg cagcgtcagg ccttgcggtg gaaaactaaa
    10381 cgccgcatct tcagtgttaa acgcataggc agcgataatc aggatgggaa aatgtaggaa
    10441 aaccacgccg ccccaggccg ccagtttgag gaaaaacggt gcgcgttcag agtgcatcga
    10501 acgctcccag acgtttcaca aacgccagat agagtgcgat gagaacaatc ggcaccaggg
    10561 tgaatgccgc cgccatcggc atattgccaa tcgccccctg ctgggaataa accatattgc
    10621 cgataaaata tcctggaggg ccaaccagct gcgggacaat aaaatcgccc agcgtgagtg
    10681 agaaggtaaa gatagagcca gcggcaatac ccgggattgc cagcggcagc accacatagc
    10741 gaaaggtttg tcgtggacgt gcgccaagat cagccgacgc ctgcaacaat gacggcggca
    10801 aacgctcaag cgccgcctgc accgggagga tcatgaacgg caaccagata tagagaaaca
    10861 ccagaaagcg ccccagcccg gaagttgaca gcgtattacc tcccaccgcc ggtagcgtga
    10921 ggaacgcagt cagcagtggt tccagcccca gatgttgtaa aaaccactgc gccacgccat
    10981 cttttgccag caataacgtc caggcatagg ctttaacaat gtagctcgcc cacatcggca
    11041 acattaccgc gatataaaaa aacgctttca tttttccgct ggtatagcgc gccatatacc
    11101 acgccattgg aaaagccaga atggcgctgg cgatagtgac tgccacagcc atggtcagcg
    11161 tgcggagaat gatgtcgtaa ttcgccggat taaacagcgc acggatattc gccagcgtca
    11221 gttccggcgt taccgacatg gtgaaatcgt caaaggtata aaagccctgc cacaacagcg
    11281 tcagcagcga gccgaaatag acaatgccaa accacattag cgggccaagc agcagcaaaa
    11341 acagccccag ccccggatta cgccagaaga aaccggacac cttacccaga cctggacgtg
    11401 aaggtgattg caatacgttc atcgccattc acctctcctc aaccagcgcc accatcacat
    11461 cacgcgacca ggaaaccatc acctgttgtc cgggcgtgag cgtggcaggc agttcttcgc
    11521 ctgtcatatt ggcctgactc accagcagtt tttcaccgcc gctcagtgtc agttcaaaac
    11581 gagttgccgc gccctgatac tgcaccgcct ggatcgtgcc attagcctgc atttcgccag
    11641 gggtgttgag gcggatatgt tccggtcgta gtgcgaagct tcccgtcatg ccacaaagtt
    11701 tctctgccat cagtccatca aaaacattcg atgtcccaac gaatcccgca acaaacggcg
    11761 tgcgcgggcg catatagaga tcacgcgggg aatcgacctg ttcaatgcgt ccattattga
    11821 aaaccgccac ccgatcggac atcgataacg cttcgccctg atcgtgggta acgaagataa
    11881 aagtgatacc gagagactgt tgcagctttt tcagctcaag ctgcatttgc tctcgcaatt
    11941 tgagatccag cgcgccgagc ggttcatcca gcaacaacac acgcggttcg ttcaccagtg
    12001 ctctggcgat ggcaacccgc tggcgctgac caccagaaag ttgtgacggt ttacgttgct
    12061 gtacaaaccc caacgccact ttctcaagcg cctcttgcgc cattgcatgc cgctgctttt
    12121 tattcacgcc tttgaccatc agcccgtagg ctacgttgtc aagaatcgac atatgcggaa
    12181 atagcgcgta gtcctggaag acagtattca cgtcccgctc ccacggtggc agattgctgg
    12241 cgggcgtacc aaaaatagag atagccccgc cggaaagctg ttcgaatcca gcaatcaggc
    12301 gcaggcaagt ggttttgccg gagccggacg gccccagcat agagaagaac tcaccatctt
    12361 ttatcgcaat actgacgcca tctactgcgc gcacgtcacc gtacaaccgt gagacgttgt
    12421 caaactccac tgcgtacgtc ataaaacacc ccagcgaagt tagcgaccgc ccataatggc
    12481 aatgtaatcc tgcgtccagc gactgtaggg aacaaacttg cccccttctg ctataggcgt
    12541 tttccagaag gcgattttat cgaaatagtt aaaaccgttt gtttcacaac ctttctcgcc
    12601 taataacgga ctggctttac acccttgcgg cactactggt aacgagccaa accaggccgc
    12661 cacatcgccc tgcacttttg gggttaatga ccagttcatc catttgtagg cgcaaaccgg
    12721 atgtttcgct tcgctatgta gcatggtggt gtcagcccaa ccggtaacgc cctccttcgg
    12781 gaaaacggta gcgacaggct ggccttcggc tttcagggcg ttggcctgat agggccatgc
    12841 actggaagcc accacaccct cgtttttgaa atcgctcatt tgcacggtag tgtcatgcca
    12901 gtagcgatgg atcaaattat gttgatcgcg cagcactttc agcaccgcct gatactgttc
    12961 ttcggtgagt tgatacggat cactgatgcc caactgtggc tgcgtggctt tgacgaacaa
    13021 cgcggcatcc gcaatgtaga tagggccatc ataagcctgt acccggcctt tattgctctt
    13081 gccgtccggc agattctgct caacaaaaac cacttgccag ctatccggcg gcgtcgggaa
    13141 ggttttagtg ttgtacatca gcaggttcgg cccccattgg taaggtgtgc cgtaaacttt
    13201 gccgccaacg ttaaaccagt cgcctttaac cacgcgcgga tcgagcgctt tccagttggg
    13261 aatcaaggcg gtattaattg gctgcacgcg tttgcccata atcaaacgca gcgaggcatc
    13321 gccggatgcc gtaaccagat cgtaaccccc tttggccatc agactgacca tttcatcgga
    13381 agtcgcggcg gttttaacat tcaccgcgca gcctgtctct ttttcgaatt gcgttaccca
    13441 gtcgtactgt ttatcagttt gtccgcgttc gatgtatccc ggccaggcga taatatccag
    13501 tcgcccttcc ggattctcta aattggtagg cggttcggcg gcgtgagcgg tcattattgt
    13561 catgctgagc gcacacaggc tgctgcgggc aaatgtcttg ctcataaggt cttactcctg
    13621 tcgtaattaa ttgttcagcc gccgtgccgt aaaaatatct ccttgtttaa ccatagacgg
    13681 cggcttgcag gcacaccttc ggccctgaga aaatttaatc aggttgtgag ctaccgcgca
    13741 ttatgccgtc tgtaaggtta tcttctggag tatagacaag gagttaggat atatgactgc
    13801 tattcgaaat tcatattacc caacctgaga ggaattaaat taaacgctat tacagccgtt
    13861 cttgaataag tttgcctaat tgttttaccg cctgttcttc acgttctccc cactgccacg
    13921 cggtattaaa acgaaaaaaa cgtgaccagt tttcaccggt agaaaacata ttacccggcg
    13981 caatactgat atgatgcgtc agtgccacca ggcttaattc tcctgcatct aacggctcgg
    14041 ggagctccag ccagagaaaa taaccgctgt cattgtgatg aattttgact tccgcaggca
    14101 gataacgcag caaagcctgc caggcgtgtt gtttacgttc cgcaagctgg cgacgcagac
    14161 gacgaagatg agcatcgtat cggcgagtag aaaggtaatc caccagcgca agctgcatcg
    14221 gtgagctggt ggaaagcgta ctcatcaact gcaagcgttg aattttacgt gcatgttttc
    14281 cggcggcgac ccaaccaata cgaaaaccag gcaccagaca tttcgaaaac gaagagcaat
    14341 gcaaaacacc atcgtggcga tcccacgctt tcgcaggcag cggtttttcc cgtccaaaat
    14401 aaagttcgct ataaacgtca tcttcaatca gcgttacgtt gtactgattg agcaacgcta
    14461 ccagccgcac ttttttctgc ggcgttaagg taaatccaag tgggttctgg ctattagtca
    14521 tcagccagca cgctttcacc ggatactcct gcaacgccag ttccagcgcc tgaagatcga
    14581 tcccttcttt aatatccgtc gctaccgata acgccttcag ccgtaaccgc tccagcgcct
    14641 gcaacgcgcc gtagaaacaa ggattctcta ctatcaccca atcgcccggt tcagttaccg
    14701 cttgcaaact gaggtttaat gcctctaacg ccccggcggt aatgacaatt tcatcaggag
    14761 aaatggtgat gccctgtaag gcatagcgac gagcaatagc ctgacgcagt tctacgtttc
    14821 ctggcggtaa gttttcaatc acgctcatcg ccgtggcggt tttgcttacc tgcgccagcg
    14881 agcggtttag ttgttggagg gggaaaagtc gcgggtcggg aaaggccgag gcaaacggaa
    14941 ctaccgacgg atcgcgactg gcctgcaaca tatcaaaaat ataggtgttg atatcgactg
    15001 cttcatctcg tgtgactgga atgactgtcg ctttcggcat ttttattgcc tgtggcgcaa
    15061 cgtaataacc cgattgcggt cgcgcgataa tatatccctg actttcgagc aactgatagg
    15121 catggctgac agtcataaag ctcatgccag aaagcgccac ctggtcacgc aacgaaggca
    15181 aacgatcgcc gggttgccag atacccgacg caatctgctc gcgtaattgt tctgcaagct
    15241 gctggtattt tttcattatc gaatcgtaat tatgtgcaga tgattcggca gtctatatca
    15301 gttgttaaaa atgacaactt ttcgttaact gtaattaaac catcaccagc gataactctt
    15361 tgccaagcgc attagccgcg agctggacgg cgtcgatttt tgtcacatgg tgcaaattaa
    15421 ataggcgagt aatctcctgt ttatgtttgc caattcgcct ggctaactct tgctgagtga
    15481 tttctgactg taaaaaagca tttaacagca ataccttaga ggcgacgctc aaaggcactt
    15541 caataaagtg atcgtgacta tttaatggcg aagtttacct ccgtgtgtaa tgtgctcgaa
    15601 aatagcacta aatcgccgca acggcagaat gcaatgcaaa atcaggaagg aagattccag
    15661 aatttctttg ctctaatttg caaaactgaa taataacctc aagaaacgat cggcatcata
    15721 gcggcaaaag gttagaaaaa ggtggcggaa tgttttcgaa ggcgttatcg gttgtcttat
    15781 taacgtgtgc actgttctca ggacaactca tggcagggca caaaggacat gaatttatgt
    15841 gggtaaagaa tgtggatcat cagctgcgtc atgaagcgga cagcgatgaa ttgcgtgctg
    15901 tggcggaaga gtcggcggaa ggtttgcgcg agcattttta ctggcaaaaa tcgcgcaaac
    15961 cagaagcggg acagcgttga gtcatttgtt gccggatgcg gcatgacgcc ttacccggca
    16021 ggcaatatca tgcagatttg atgagtggtg agtaacccga aaactggcgt tttacccttt
    16081 tcgcttcggc aatgttttca gcagatcatc cggacttacg gatgccacta cgcttcccgg
    16141 taacggcatt ttcagtatgt gatttttgat tttgccaatg acgtgcatct cgcatgggcg
    16201 gcaatcaaac tttagcgtta atacttcatc gccgttaacc agttgcattg gcgtcgcctt
    16261 ccagcttttg atattgcctt tcgcctgttt cgggcacaga ttgaaggcaa agcgcagaca
    16321 atgcttggtg atcatcaccg ggacttcgcc cttctcttca tgtgcctcat acgccgcgtc
    16381 aatcagttgc acaccatagc gatgataaaa ttcacgcgct ttctggttgt atacgttcgc
    16441 gaggaaactc agatgcgttt gcggataaac cggcgcagga tcagcaaccg gtttacggct
    16501 gccgcgctgg taactggcaa gacgcgcagc atcaagcata tcggcggctt cacggcggaa
    16561 ctggtttaac agactgtttg gtacaaacag cgcccccggc aaattaattt gcacattgcg
    16621 ggcgtaatag atggtttgcc ccagttttgc cagaccatcc ttcagattgt tcattgcttt
    16681 ttcggcgtta ttggcttcgt cgaactgccc gtccagcgta tgtgtgatgc tgacaccctc
    16741 ttcactggtg agggtcagaa tcagttgttc ctgccagccg cccagttcaa tgtctaccgc
    16801 cacccggcgt tcgctggagg tttttgtcag tgcctgctgc cagttatgat caagatttcg
    16861 gtttagtggg tgatgcgggc gaattttgtg caaatctgct ggcatttcat tgggccagac
    16921 gcggtactga ttttctccgg ttttctctac cgtattagca cgaaaaccga cgacttcacg
    16981 tttaatcatc acgttcaggc catcgccatt tgccagtggc tcggtaacgg caacatcgag
    17041 atgatctttc gctactttca atacttcgcc caccggcagg ccgataaatt tcggcgaatc
    17101 gaacgcgcca atatcgcctt tacgggcatt cacaaaataa tctgtgctac cacggtggaa
    17161 agtcttttcc gtcgatggaa caaagaaatg ttcagtacga cctgatgaag cgcgcgccag
    17221 atcgccacgt tcttcaataa tagcatccag catctgacga taatgggcgg tgatattctt
    17281 cacgtagctc atatctttgt aacgcccttc aatcttgaag gagcgtacgc cagcatcaat
    17341 cagcgcgccg aggttggcag tctgatcgtt atctttcatc gacagcagat gtttttcata
    17401 ggaaaccacc cgcccctgat cgtctttcaa tgtgtatggc aaacggcacg cctgcgagca
    17461 atcgccacgg ttggcgctac gccctgtttg cgcatgagaa atgtagcact gacccgaata
    17521 ggccacgcac aatgcgccat gaataaagaa ttcaatggtc gcgtccgtgg cctggtgaat
    17581 cgcgcggatc tgctcaagat tcagctctcg cgccagcaca atctgcgtga agccaacatc
    17641 agagaggaac ttcgcttttt ctactgtacg aatatcgcac tgcgtactgg cgtgcagttc
    17701 aatcggcgga atatcaagtt ccagaatccc catatcctga acaatcagcg catcgacgcc
    17761 ggtctgatag aggtcggtta tcagccgttg cgcgggttcc agctcatcat catgcaagat
    17821 ggtgttaagt gtgacgaaaa tttttgcacc ataacgatgt gcaaacggca ccaactcggc
    17881 aatatctttc aggctattac tggcattatg acgggcacca aaaccagggc cgccgatata
    17941 aacagcatcg gcaccgtgca aaatagcttc gcgggcaatg gcggcatcgc gtgccgggct
    18001 taacagttca agacgatgag aagatacggt cactttctta tccagtagct gaaaaatggc
    18061 ggctatttta gccacaagcg cgacgttgcg aaatagtttt cccgcttaac tgcgcgggta
    18121 atggatgagg gaatgaaaat gtacggtttg ctccccacca ttacgataga catgcgtgac
    18181 gtctgcagca aatcgaacgc cttccccaca attaaggctc tgccattcac catcaacaca
    18241 cagatcaagt tgtccatcaa tgacgacgac atgttcgatt accccctttt catggggcgt
    18301 cgattcgctt attgcaccgg gtgccatctg aatcgagaag tgttcaaagc agagttgtgg
    18361 atcataagga aacagcgaag tgatcaccat cgcctgccgt tgtggatcat aaactgaagg
    18421 cgtagaggac tgcggcggag aaataaatgt tgaaaatggc acgttcaacc cggtggcaat
    18481 tttccataat gtcgcgaccg tcgggctgga ttcattacgc tcaatctgcc ctaacatcgc
    18541 tttagaaacg cccgtcgctt ctgccagtcg cgaaagactc cagccgcgct gctgacgtag
    18601 ttgtttaagt gtggtggata aaaagcgagc gagattttcc atgcgactcc tccggcaaaa
    18661 cggaagttta tcacttgtgc gttataacgg acaaatgcta cggtacctgt acgttataac
    18721 gcacgaggtg actatgcgtc tgttttctat tcctccaccc acgctactgg cggggtttct
    18781 ggcggtatta attggctacg ccagttcagc ggcaataatc tggcaagcag cgattgtcgc
    18841 cggagccacc actgcacaaa tctctggctg gatgacggcg ctggggctgg caatgggcgt
    18901 cagtacgctg gctctgacat tatggtatcg cgtacctgtt ctcaccgcat ggtcaacgcc
    18961 tggcgcggct ttgttggtca ccggattgca gggactaaca cttaacgaaa ccatcggcgt
    19021 ttttattgtc accaacgtgt taatagtcct ctgcggcata acgggactct ttgctcgtct
    19081 gatgcgcatt attccgcact cgcttgcggc ggcaatgctt gccgggattt tattacgttt
    19141 tggtttacag gcgtttgcca gtctggatgg tcaatttacg ttgtgtggca gtatgttgct
    19201 ggcatggctg gcaaccaggg ccgttgcgcc gcgctatgcg gtaattgccg cgatgattat
    19261 tgggatcgtg atcgttatcg cgcaaggtga cgttgtcaca actgatattg tctttaaacc
    19321 cgttctcccc acttatattc cccctgattt ttcgtttgct cacagtctga gcgttgcact
    19381 cccccttttt ctggtgacga tggcatcgca aaacgcaccg ggtatcgcag caatgaaagc
    19441 agccgggtat tcggctcctg tttcgccgtt gattgtgttt actggattgc tggcgcttgt
    19501 tttttcccct ttcggcgttt attccgtcgg tattgcggca atcaccgcgg ctatttgcca
    19561 aagcccggaa gcgcatccgg ataaagatca acgttggctg gccgctgccg ttgccggcat
    19621 tttttatttg atcgcaggtc tgtttggtag tgccattacc gggatgatgg ctgccctgcc
    19681 cgtaagttgg atccagatgc tggcaggtct tgcgctgttg agtaccattg gcggcagttt
    19741 gtatcaggcg ctgcataatg agcgtgagcg cgacgcggcg gtggtggcat ttctggtaac
    19801 ggcaagtgga ttgacgctgt tcgggattgg ttctgcgttt tggggattaa ttgccggagg
    19861 cgtttgttac gtggtgttga atttaatcgc tgacagaaac cgataataaa aagcgccatc
    19921 taacgatggc gcgtctggaa ctactttttg ttaacgtcaa acatggtggc atcggtcgcc
    19981 atgtcatcaa taacttgttt aatattttcg aaagccattg gcgtactttc gttattcagg
    20041 tctttacctt cgccctgacg aacgaccttg ataaccggtt tattagtcgc tgcatcaatc
    20101 agctcacctt caaaatacaa gcgagtatcc atggtacggt ggcctgtagc catttgcgtc
    20161 cccgcaacca ctaatgcaac aggaaccact tcatagaatt gcagcccttc tttactggta
    20221 tctacaccgg taatggcccc acggaaaatc agactacgcg gcccagcggt ggtaaccagt
    20281 ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg tatagttcaa aattttatcc
    20341 agaactttct gccctacctg ggtagaaggt ttcggtaccg gataataagt gattgggttc
    20401 cagacgatgc tgtcatattt gctttgatca aaactcgggt ctacccaacg taaaacaggt
    20461 ttacctgttg ccgaggttgt ttcttttaaa tcagagtaat tgtttaaaaa accagaatat
    20521 ttatctggct gggtgatttt agaggcacaa ccagacagag ccaacaagcc gcataaagct
    20581 gcaactttcg caaatgatgt ggtacgcatg aatagtttcc tgagatgatt atattacttt
    20641 ctaagttata gcaaaaagcg ccgcttcgtg ttgtggcaaa aaaagtgttg gtgaaaataa
    20701 attttttagc cccttttcag acgacatagt caatgccgaa aaaggggcta agtcaatcat
    20761 cagtagttaa atttcagtaa gttaagcggc taaaaaactt cgactactgt gattaatttc
    20821 acatcccttc gataaaaaat atgcttaccg aaaagaagaa cagtaaacca tctcagcgtg
    20881 gggaattttc acgcgctttg ttcttaccgg atcatttttt acgtgccagc atcgtggcga
    20941 aacgcagttt aatacgatta ccgttggcgt cggtgcggtg cagctcgccg acgtcttcat
    21001 tgtatttcac cctctcccag ccttcgtaat atcgacgtaa ttccccctct ttgaaggcaa
    21061 acgggaagcc gacggtacat ggataatcag cggtatccat cgccgccaca atcaggttgt
    21121 aaccgccagg tttagtgcaa cgttgcatat tggcaatcag ccctgggatg gttttagcct
    21181 caaggaacat cagcaccaca gtcgaaagaa taaaatcgta ctgtccatca aatgtgaggt
    21241 tattcagatc aacgacccgg gtgtgtaaat tatccagatt ttcaatggac ttaatgcgct
    21301 cgacgttggc gatactcatg gcatttttat cccatgcgtc aacatcataa ccattggctg
    21361 ccaggtaaag actgttacga ccattgccac agcccagatc cagcgtttta cccggtttaa
    21421 ccactttcac cgcttccagc acttcagagt gtgtgcgggt taattcatat ttatcagtaa
    21481 aatagttttc atcacgaatg atcattcttt gtcctctgct ttcattaaaa cggcgcgctc
    21541 ggttctgact aacaattttc cctgcatcag aagcgcaaaa gtacggatga gcagtattgc
    21601 aataataaaa ttggtaaaga taaacagcgg caccgccagc gtatggaaaa atccattatc
    21661 gctgccactc cccagatgca aaccggtggt tgccagtgca gatacaccga acgagaaact
    21721 ccagaatgaa gcattaaatg gctgggagag ataccatggc atcagacgta gcataaacag
    21781 cagttgcagc agttcataac cgaaaagcat tttcgccagc gtgtcaccct cgccgccgtt
    21841 gacgctcagc caggcactac aggccaccag cgcaggagcg agctgaatgc cgagtgatgt
    21901 ccgcagtgcc gtgggtaatt ctcccgaact gcgcagacgc tgcaagatca ccggttccag
    21961 gcttagccat gagaaaacgc ctgcgcctaa aaacaccaga ccggcgtcgg tgtagcccaa
    22021 cgcaccacag gccattgcgc tgataaagtt gttggcaact gtcggcagat acagtccagg
    22081 cgtggtagct tcttcagggt gagatccgcg ccataatccc gcagtttgcc aggcggcata
    22141 agccaactga accacgacac caaaactgaa caggcacacc gccagtgggc gaaaccacgg
    22201 aacaaaacca atcgccacca gcatcgtcgt tgccggaaac aaactcacaa aactgctcag
    22261 cactggatgg cgcacttctg ccagcacgct atgcggaaag cgtatgagtc gggtaataaa
    22321 tgcgccagtc aataatcccc agatgatcat cgccagaatc accagcccat cccccaacca
    22381 gtggctgacc tgccaaacct ggctggcata gcgccaggca aatcccatcc ctatcgtccc
    22441 caacacaata ccaaagtagc ctgccggcaa attgagcact ttatcgctct gcatcttctt
    22501 actcattttg tttaatttat aaagtatatt tgaaatgcat tttatggaat tttctcgagc
    22561 atagccagag ccgcagaatt tgctacggtt atagtaagtc acctgtggga aaacgctatg
    22621 cgtaaatatc gtctaagtga agaacagcga gcctttagtt atcaggaaga tggcactaaa
    22681 aaaagtgtgt tattacggca aattatcgcc atgagcgatt ttaacgatgt gatagcagga
    22741 agcgctggcg gctggatcga tcgcgaaacg gtgttagcac aggaaggaaa ttgctggatt
    22801 tacgaccaga atgctattgc gttcggcgga acggtgattt ccgggaatac gcgtatcacc
    22861 ggcaccagcg tgctgtgggg agaggtttac gcaacggata atgtctggat cgataatagt
    22921 gaaatcagcc agggggcata tataagtgac agcgtcacca ttagtgactc attagtttgc
    22981 ggtcaatgta gaatatttgg tcacgcctta attgatcaac attctatgat cgtcgccgca
    23041 caaggcttaa cgcccgacca tcaactcctg ctacaaattt atgatcgcgc gagggttagc
    23101 gcctcgcgta ttgtccacca ggcacaaatt tatggcgatg ccgtcgtccg gtatgctttt
    23161 attgaacatc gcgccgaagt ttttgatttt gccagtattg aaggtaatga agagaacaac
    23221 gtctggctat gcgattgcgc taaagtttac ggtcacgcac aggtgaaagc tggcatagaa
    23281 gaagatgcca ttccgacgat tcattacagt tcacaggtgg cggaatatgc cattgtggaa
    23341 ggtaattgtg tgttaaaaca tcatgtcttg attggcggaa atgccgtggt acgtggcgga
    23401 ccgattctgc tcgacgagca cgttgtcata caaggtgaga gccgtattac tggtgcggtg
    23461 ataattgaaa accatgtgga gttaaccgac cacgccgtcg ttgaagcctt tgatggcgac
    23521 acgattcatg tccgtggtcc aaaagtcatt aacggcgagg agcgcattac tcgaacgccg
    23581 ttggcagggt tattgtgaat cgataatacg cgcgtataag ttcacatcat cataggcatc
    23641 attcaggaac tcagcctgtt tcaggcaacc ttcaaggata aaaccattgc gcaaagcgac
    23701 ctggttgctt tgcgcattgt ccacccgaca tttgatcacg aagcgtctaa gttcaccaga
    23761 ctgagcgtaa tgatgaatca atgcctgcag cgcctgagaa atgatcccct gcccctgatg
    23821 agattcgtcg agccagtagc cgatttcagc ggttttattc agtggttcaa tacgattaaa
    23881 agagataacg ccgataagtt catcttcttt gaaaatcatg aacattttgg catagccgcg
    23941 ttgatgcaac atcacattac cctgcaccgt ttttcgcgtg tcctcttcac tttgaacaaa
    24001 ctgcggccag tttagcgact gctgtaacca ggttttattt ttacagatta actgataaag
    24061 aggtgtgaca tgactttccg caacagcatg taattcaagt gattcgctta cttttatcgt
    24121 ttcagtcatc ttatctccag tcaatccatg cagctgttaa aaaagccgcc tggctggcgg
    24181 cttaggaaag tttacacctc tttgacgctc acctgctgca ggattttgag catctcatct
    24241 tttaactgta gcttttgttt tttcatgcgg accacttccg cattgtaccc tcgaccgtcg
    24301 gaaccttcct ttctggcaat ttcatgatca agtttattgt gtttatcgaa caaggacata
    24361 aagcgaggat tttcgttttt cagacgggat attaaatctc ggtattctgg aaacatacgc
    24421 actccctgtt agtggctgac aaggtgtatc tgaaatacac accatcagat tactcattta
    24481 acttagggaa aaatgcgagc gggatcgtgt ttaaccagac ggcacctccc gaagaagatg
    24541 ccgaagaaaa aacgattaac tcatcacgcg gtcatcaaca tactgacgtt tatccggcgc
    24601 tggcgggaaa tactgataca gccatgtttc actgatagcg tcgccctgac aacgcagata
    24661 catccgcata tcgaccggat cagtggagtc cgaagtcgga taccagtcaa actgaatacg
    24721 ataaccatca atgggttcaa tatagagaat ttcgatttgc ttcgcttccc cactggaaag
    24781 cgtaatcacc ggctcaatgc cttttggcgc ggcagctttc agatcaccac caacgaaatc
    24841 gacggcaaaa cgtcgcgccc atttttcggg atagtgttca cctggcgccc aaccttccgg
    24901 gaaaccgccc atgccggtac gcgtcgccat aacgcgcgct aatgggcaat gaacaggcgg
    24961 ttgcgcactc cagtacagac gatactggaa tgcaaactca tcacccgctt ttacagcttt
    25021 ttctggctgc cagaagcaga caatgttatc cagcgtttcg cccgttgttg ggatttccat
    25081 caggccgatg gtgcccttac cccacttgtt acgcggttcc acccacagac ttgggcgttt
    25141 gttataccag cccataatgt cctgataatg ggagaaatca cgatccagtt gcaataaacc
    25201 aaaccctttc gggttgttgt cggtgtaagc attgaactgc aatttttgcg gattgttcag
    25261 cggacggcaa atccactcgc cgttgccccg ccacatggac agacgatcag agtcatggat
    25321 ttgcggatga atggtgtcgc acatccgacg ttcattagta ccgcagctga acatactggt
    25381 catcggcgca atgcccagct gtttaatgtc tttgcgcgca tacaggtgat tttccacatc
    25441 cataatcacc tgatttttct cacaatgtat agtgaactta taggcaccag taatactggc
    25501 gctatcgagc aacgcataaa cggtaaaggt ggttgcccct ggttttaccg tatcaaacca
    25561 gaaggcggta aagtcgggga actcttcttt gctgtcggtg taagtgtcga tcgccaggcc
    25621 gcgggccgac aaaccgtatt gatatgtgtc atcaacggcg cggaaataac tcgcgccgag
    25681 gaatgatact acatcacggc gcgccagttc gggggcttta aacacgcgaa aaccggcaaa
    25741 accgagatcg ctttgccctt ctaattgttt ggtatccaca cccgcatcgt tgtatttgaa
    25801 caactccggg cgaaagtgaa tttcacgcgc cagatgtgtt gctggatcta cagaaaacat
    25861 acgaacgcgg cgacggaatc ccattcccat atggaaaaac tgagcgtcca gttgacggtt
    25921 ctcaacgtta tgccagagcg atttttcggc gtcgtattga atactgttat aagcctgcgg
    25981 cgtcattgtc gccaaagtgt caggtaacgg acgtggtgca ccacgccatg ctgtttgcgc
    26041 taagtcgtgc gccattgact gtagaatgga gaagtcaaaa cgctgggttt gcccgtcggc
    26101 aatatcagaa tcggccgcga atgccgcctg agaaaaaaga gaagcaatgc cgctggtacc
    26161 gcacacggcg gccatagcca ttgaaccttt aataaatcgt ctacgatcca tacctgaaag
    26221 taagtccttc tggtgttgtg aatggtcccg cgctggtcat tttcagcggc aagaaagt
//
LOCUS       DADTFG010000062        25890 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.062, whole genome shotgun sequence.
ACCESSION   DADTFG010000062 DADTFG010000000
VERSION     DADTFG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.062"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..289)
                     /locus_tag="J1138_002359"
     CDS             complement(<1..289)
                     /locus_tag="J1138_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA8454147.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGA"
     gene            complement(360..2474)
                     /gene="fusA"
                     /locus_tag="J1138_002360"
     CDS             complement(360..2474)
                     /gene="fusA"
                     /locus_tag="J1138_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBA8454148.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2571..3041)
                     /gene="rpsG"
                     /locus_tag="J1138_002361"
     CDS             complement(2571..3041)
                     /gene="rpsG"
                     /locus_tag="J1138_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBA8454149.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3138..3512)
                     /gene="rpsL"
                     /locus_tag="J1138_002362"
     CDS             complement(3138..3512)
                     /gene="rpsL"
                     /locus_tag="J1138_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBA8454150.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3638..3925)
                     /gene="tusB"
                     /locus_tag="J1138_002363"
     CDS             complement(3638..3925)
                     /gene="tusB"
                     /locus_tag="J1138_002363"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBA8454151.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3932..4291)
                     /gene="tusC"
                     /locus_tag="J1138_002364"
     CDS             complement(3932..4291)
                     /gene="tusC"
                     /locus_tag="J1138_002364"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBA8454152.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4291..4677)
                     /gene="tusD"
                     /locus_tag="J1138_002365"
     CDS             complement(4291..4677)
                     /gene="tusD"
                     /locus_tag="J1138_002365"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBA8454153.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRSWQQLNMQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQTGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4677..5399)
                     /locus_tag="J1138_002366"
     CDS             complement(4677..5399)
                     /locus_tag="J1138_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8454154.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5566..6378)
                     /gene="fkpA"
                     /locus_tag="J1138_002367"
     CDS             complement(5566..6378)
                     /gene="fkpA"
                     /locus_tag="J1138_002367"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBA8454155.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6599..6817
                     /locus_tag="J1138_002368"
     CDS             6599..6817
                     /locus_tag="J1138_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBA8454156.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6866..7456)
                     /gene="slyD"
                     /locus_tag="J1138_002369"
     CDS             complement(6866..7456)
                     /gene="slyD"
                     /locus_tag="J1138_002369"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA8454157.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7551..7751)
                     /locus_tag="J1138_002370"
     CDS             complement(7551..7751)
                     /locus_tag="J1138_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBA8454158.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7761..9566)
                     /gene="kefB"
                     /locus_tag="J1138_002371"
     CDS             complement(7761..9566)
                     /gene="kefB"
                     /locus_tag="J1138_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBA8454159.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9566..10117)
                     /gene="kefG"
                     /locus_tag="J1138_002372"
     CDS             complement(9566..10117)
                     /gene="kefG"
                     /locus_tag="J1138_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBA8454160.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGC"
     gene            10248..12161
                     /locus_tag="J1138_002373"
     CDS             10248..12161
                     /locus_tag="J1138_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8454161.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPVSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12161..13183
                     /locus_tag="J1138_002374"
     CDS             12161..13183
                     /locus_tag="J1138_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA8454162.1"
                     /translation="MAQITTTDANEFSSSAKFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHTEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLTQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13177..13395
                     /locus_tag="J1138_002375"
     CDS             13177..13395
                     /locus_tag="J1138_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBA8454163.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVSDVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13449..14318
                     /locus_tag="J1138_002376"
     CDS             13449..14318
                     /locus_tag="J1138_002376"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBA8454164.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14373..14777)
                     /locus_tag="J1138_002377"
     CDS             complement(14373..14777)
                     /locus_tag="J1138_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBA8454165.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15079..15711
                     /gene="crp"
                     /locus_tag="J1138_002378"
     CDS             15079..15711
                     /gene="crp"
                     /locus_tag="J1138_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBA8454166.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15762..17852
                     /locus_tag="J1138_002379"
     CDS             15762..17852
                     /locus_tag="J1138_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454167.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPDKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17915..19138)
                     /gene="argD/dapC"
                     /locus_tag="J1138_002380"
     CDS             complement(17915..19138)
                     /gene="argD/dapC"
                     /locus_tag="J1138_002380"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBA8454168.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAMAKVIGGK"
     gene            complement(19224..19787)
                     /gene="pabA"
                     /locus_tag="J1138_002381"
     CDS             complement(19224..19787)
                     /gene="pabA"
                     /locus_tag="J1138_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBA8454169.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19819..20421)
                     /locus_tag="J1138_002382"
     CDS             complement(19819..20421)
                     /locus_tag="J1138_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBA8454170.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20411..20578)
                     /locus_tag="J1138_002383"
     CDS             complement(20411..20578)
                     /locus_tag="J1138_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBA8454171.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20683..21255)
                     /gene="ppiA"
                     /locus_tag="J1138_002384"
                     /gene_synonym="rotA"
     CDS             complement(20683..21255)
                     /gene="ppiA"
                     /locus_tag="J1138_002384"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBA8454172.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21526..22707
                     /gene="tsgA"
                     /locus_tag="J1138_002385"
     CDS             21526..22707
                     /gene="tsgA"
                     /locus_tag="J1138_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBA8454173.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22969..25512
                     /locus_tag="J1138_002386"
     CDS             22969..25512
                     /locus_tag="J1138_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HBA8454174.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25509..25835
                     /gene="nirD"
                     /locus_tag="J1138_002387"
     CDS             25509..25835
                     /gene="nirD"
                     /locus_tag="J1138_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBA8454175.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
BASE COUNT         6328 a         6989 c         6478 g         6095 t
ORIGIN      
        1 cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct acgtgcgcgt
       61 agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata ccacgagctt
      121 tttcttccgg cgcgttatcg atctggtcga atgcacgagc agcaccgccg taggttttag
      181 ccagtacggt ggtgattgca gcggtcagag tagttttacc gtggtcaacg tggccgatag
      241 taccaacgtt aacgtgcggt tttgtacgtt caaatttttc tttagacacg gctatattcc
      301 ttactatagt gctctcccct tcaggagaga gcacgggact ttggtattaa cccttaggct
      361 tatttaccac gggcttcaat tacggcctga gcaacgttac tcggcgcttc atcatacttc
      421 aggaattcca tagtgtatga tgcgcgacct ttggtcagag aacgcagctg agttgcatat
      481 ccgaacattt cagacagcgg tacttcagcg tggatcttaa cgccagtaac ttcagattcc
      541 tgacctttga gcataccacg acgacggctc aagtcaccga taacgtcacc ggtgttctct
      601 tcaggagttt ctacttcaac cttcatgatc ggctcaagca gaactggttt agctttctta
      661 aagccttctt taaaggcgat agaagcagcc agtttaaacg ccagttcaga ggagtcaacg
      721 tcatggtaag aaccgaagtg cagacgaata cccatgtcta ctaccgggta gcctgccagc
      781 ggacctgctt tcagctgttc ctggatacct ttatcaacgg ccgggatgta ttcgccaggg
      841 attacaccac ctttaatgtc gttgatgaac tcgtagcctt tcgggtttga acccggctcc
      901 agcgggtaca tgtcgataac aacatgacca tactgaccac gaccaccaga ctgtttcgcg
      961 tgtttacctt caacatcggt aactttctgg cggatagttt cacggtaagc aacctgcggt
     1021 ttacctacgt tcgcttcaac gttgaattca cgcttcatac ggtcaacgat gatgtcgagg
     1081 tgcagttcgc ccatacccgc gatgatggtc tggttagatt cttcgtcagt ccatacacgg
     1141 aaagacgggt cttctttagc cagacggccc agagccagac ccattttttc ctggtcagct
     1201 ttggttttcg gttcaactgc gatggagatt accggctcag ggaattccat acgttccaga
     1261 atgatcggcg catccgggtc acacagggtg tcaccagtgg ttacgtcttt cagaccgatt
     1321 gcagctgcga tgtcgcccgc gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc
     1381 tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg agttcagtac ggtatcacca
     1441 gagttaacca caccggagta cacacggaag aaggtcaggt taccaacaaa cgggtcggta
     1501 gcgattttga acgccagtgc agagaacggc tcgtcatcac ttgcgtgacg ctcagccgga
     1561 gtgtctttac cgtcgtccag gataccgttg atcgcaggta cgtcaaccgg ggatggcagg
     1621 taatcaatta ccgcatccag catcgcctga acacctttgt tcttgaacgc agaaccacag
     1681 gttaccagga tgatttcgtt gttcagaacg cgctgacgca gagcaccttt gatttctgct
     1741 tcagtcagtt cttcaccacc caggtatttt tccatcagct cttcagaagc ttcagctgcg
     1801 gattcgatca ggttctggtg ccattcgtta gccagttcaa ccatgtctgc cgggatatct
     1861 tcgtattcga aggttacgcc ctggtcagcg tcgttccagt tgatagcttt cattttcacc
     1921 aggtcaacaa caccggtgaa atgttcttca gcaccaatcg ccagctgcag cggaaccggg
     1981 ttcgcgccca gacgggtttt gatctggtta acaactttca ggaagttcgc acccatgcgg
     2041 tccattttgt taacgaacgc aatacgcgga actttatatt tgtttgcctg acgccatacg
     2101 gtttcagact gcggctgaac accaccaact gcgcagtaaa ccattaccgc accatcgaga
     2161 acacgcatgg aacgttctac ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg
     2221 ttgatgcgat gcggctcata ctgcttagcc ataccagacc agaatgcagt agtcgcagcg
     2281 gaagtgatgg taataccacg ttcctgctcc tgctccatcc agtccatggt tgcagcgccg
     2341 tcatgaactt caccgatttt atggtttaca ccggtgtaga acagaatacg ttcggtagta
     2401 gtggttttac cggcgtcgat gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt
     2461 gttgtacgag ccatttgttt cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa
     2521 gcgggctgct tactggaagc gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg
     2581 tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa cgtcttcacg tttcttaact
     2641 gcagtacctt tgttttctgc agcatcagaa agttcgttcg ccaggcgcag agccatggat
     2701 ttatcaccgc gtttacgagc agcttcaacg atccaacgca ttgccagagc attacgacga
     2761 accggacgga cttcaactgg tacctgataa gtagaaccac caacgcggcg agacttaact
     2821 tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg cttccagttc agatttacca
     2881 gagcgctgag ccagggtctc cagcgcgctg tatacgatag attcagcagt agatttttta
     2941 ccatctacca tcaggatatt tacaaattta gccagcagtt ctgatccgaa cttcggatcc
     3001 ggcagaattt tacgctgacc aatgacgcga cgacgtggca tggaaatact ccgttgttaa
     3061 ttcaggattg tccaaaactc tacgagttta gtttgacatt taagttaaaa cgtttggcct
     3121 tacttaacgg agaaccatta agccttagga cgcttcacgc catacttgga acgagcctgc
     3181 ttacggtctt taacgccgga gcagtcaagc gcaccacgta cggtgtggta acgaacaccc
     3241 gggaggtctt taacacgacc accacggatc aggatcacgg agtgctcctg caggttgtga
     3301 ccttcaccac cgatgtagga agtcacttcg aaaccgttag tcagacgaac acggcatact
     3361 ttacgcagcg cggagttcgg ttttttagga gtggtagtat atacacgagt acatacgcca
     3421 cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt
     3481 ggtttgcgta ccagctggtt aactgttgcc attaaatagc tcctggtttt agcttttgct
     3541 tcgtaaacac gtaataaaac gtcctcacac aatatgagga cgccgaattt tagggcgatg
     3601 ccgaaaaggt gtcaagaaat atacaacgat cccgccatca ccaggccatc tggctggagt
     3661 gcttaaccgt aagtctgacg aaatcagtat agtcaatggg aatgatgtcg ttcgaaattc
     3721 gaccagtcaa accgcgggca ataaggtctt cgttcagagc atagacctta atgggggcat
     3781 tacgcagact ttcaaggtag cggttgccgt caactgcggc agttacgcca tcttgcaata
     3841 atagcagttc gtcaccttca ctgagcagac gcagcagcgc agcaaagtcc gtcagccagg
     3901 gtgaacgatg taatgtgtgc agcataacgc ctcaaaacct caaaataaca tcgtagttgg
     3961 cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgaccaca aaaggtgtct
     4021 gcggatctaa cccgcgttcg cgcagtgaag ccgcacaaac ccagcactgt tcaatgtcat
     4081 acagacccaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca tcgggctttt
     4141 gtcctggcag cagctgaaaa acgccatcag caataaagaa gacggccaga tcgtcagtta
     4201 atgcggaagt tgcgagtaaa gcatctaatc cctcgcggcc agccgcggtg ccatgaggca
     4261 cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt cacaggtcag
     4321 cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccgtctgaa ggtttgacga
     4381 agccagcccc agtcttccgg cttccgtttc atcaacaacg ccacggcgta atgccgccgc
     4441 tacgcagata ttcagcgcca caccatgttg catattcaac tgttgccagc tgcgcacaag
     4501 gtcaaattcg tcgcttgccg gtgaggtcaa ttggttagcg ttatagaccc cttcccgata
     4561 gaaaaaaacg ctgcttaact catggccttc tgctattagc gcctgcgcaa actgaaaagc
     4621 actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa aacgcattac
     4681 ttatcttgcc cctggaaatc accgctcttg aactggcgga tgtagagata gacagtgtgt
     4741 ttggagatgt tcaggcggtc agcaacctgg ttgatcgcgt ctttaatatc gaagatccct
     4801 ttctcgtaga gatttagcac gatctgacga tttttggcgt tattagaaac attgcgatcg
     4861 gcattcactt cttcgatggt aaactccagc gtttgggtaa ccagatcttc aacagaagag
     4921 gcaaagttga cgcttgaacc gacatccgga gtttccggcg gcacaaaagt gctcataatc
     4981 tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac acgctgttcg
     5041 cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc gcgagtaaag
     5101 tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg tagcgccagg
     5161 tcagtaattg gcgaaccaat cttccggcct gtatgttcac cgttagcaat gcgaatggct
     5221 gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc aataagcatc
     5281 gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt ctggtcaaaa
     5341 ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga cctggacatg
     5401 aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc tcattaatga
     5461 tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt atatccggcc
     5521 tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt agtgcttatt ttttagcaga
     5581 gtctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg gtttcacatc
     5641 cagcagctct acgtcaaaca ccagggtaga attcggtggg atccccggaa cacccgcttt
     5701 gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct tgatgttctt
     5761 cagaccttct gtccaacccg ggataacacc gtccagacgg aaagagagcg gttcaccacg
     5821 ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt tcactacaac
     5881 agtatcgctg tctttcgggg cttcgccttt accggcttct actacctgat aaaccagacc
     5941 agtggaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact ctttaccttt
     6001 tgcttcgtta tcagccgcgt ctttttccat cttcgcctga gcagaagact tcacgcgagc
     6061 ttcgaatgct tgcagagtct gttcgatctc ttggtcggaa agtttgctct tatcagcaaa
     6121 tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca gtttttcttg
     6181 ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat aagctgattt
     6241 ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt ttgcagcttc
     6301 agcagcaaaa gtgattggtg catgcagggc aacggccatt gtggtcgcca gcagcgttac
     6361 tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca gggttaacta
     6421 ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca cgccctatta
     6481 tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa atgagtgctg
     6541 tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga ggtgaatcat
     6601 gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg cttttcagga
     6661 aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga tggcgaaact
     6721 gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc agccagccgt cgaacatcgc
     6781 gtcgcaggct gaagaaacgc ctccgccaca ttattgaggc gtaaaaaaag cggggattcc
     6841 ccgctttttt gtcacctttt cgatattagt ggcaaccgca accgccgttg cctttaccgc
     6901 cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgacca ccgcagcaac
     6961 cgtcgtggtc gtgatcgtgg tggtgatcgt gcgcgccgtg aacgtgacca tgagccagtt
     7021 cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg ttctgaccag
     7081 ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg atttcaaccg
     7141 gtaccggacc ctggtcggtt tcagccagga aacgcatacc tacctgcagt tcatcaacgc
     7201 ccataaatac gtctttagga acgcgctgca ccaggttttc gtcgtactga ccgtaagcgt
     7261 cgttcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc agcgctgttt
     7321 ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca ctcaccggag
     7381 actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg accaccaggt
     7441 cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca gattgtagcg
     7501 gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg ctagtccgga
     7561 tgaaaaatcc cgatcacttg ctcatctttg cgaacgtgat cgcgggcttc tttgtctgct
     7621 tctcgcatct gatgtccaca cttaacacat tcaacgatat caatattatt ttcgcgccac
     7681 atcgccattg aatcctgtgc ctgacaggcc gggcattttg cgcccgcaat aaaacgtttt
     7741 cggattgcca tctttaccct ctactcaaat tcatcccagc cgtccagctg gcgtcgttct
     7801 tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct ggcgcgagaa
     7861 atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat atccaggcgg
     7921 cgaaaatgca gttgcgcgcg ctgcgcctga tgcggatgca tgccaagcgt gaccagcgtc
     7981 ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt cacccctgcc
     8041 tgtaataact catgcgcttc cacacgtccg cgcgctcgcg caagaatatg caaatgcgga
     8101 aagtgctgct ggcatatttc caccagcttc atagtgtctt ccggctcgtt acaggtaatg
     8161 acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatctacctg cgtggcgtcg
     8221 ccgtaataaa ctttgtagcc atatttgcgc atcaggttaa cggcactgat atcccgctcc
     8281 agtacagtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg accaaaacgc
     8341 ccaaagccca cgacaatgac ctgcggttta tcatcgttga cccatggttt ttcgtcttct
     8401 tcttccggtc cgttaaactg gcgggatagc catttatcga ccagcttcat cagcagcggc
     8461 gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc gccctggaat
     8521 aagcgttgtg aagaagcggt agaaaagagg acaaaggcaa actcaccgcc ctgactcaac
     8581 acgccagcaa actgcatccg ctcagaactg cgcacgccat acaatcgcgc cagcagatac
     8641 agcacgagaa ttttcaccgc caccagcaca accacactta tcactaccca caacagatgg
     8701 gtataaagca ccccgaggtt gagcgacatg ccgacagaga taaagaacaa accaagcagc
     8761 aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact ttccgccagt
     8821 agcacacccg caataaacgt accgagcgcc atcgacaacc ccagcgcatc cataaacaat
     8881 gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac accagaagct
     8941 gcaataaaac ggaataccgg acgcagtaaa tagcgcccgc caatcagcat gccgacaaac
     9001 gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga acccgccaac
     9061 aacggcacta acgccagtgc cgggatcacc gccagatcct gaaacagcag taccgagaaa
     9121 cctaactggc cggattcgct gcgattcatc cctttctcac gcatcaattg caacgccatt
     9181 gcagttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca tgagaaatgg
     9241 gtcagcatca ataatcctgc cagcaacgcc gcgcttaaca gcacctgtgc cgcgcctacg
     9301 ccaaaaatcg aacgccgcag ttgccaaagc ttggagggat tcaactcaag gccgatgata
     9361 aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac gtcgctaata
     9421 aaccccagcc cccacgggcc aatcgcaatc cccgccagca aatatcccaa cactgcacca
     9481 atacccagcc gcgatgccag cggcaccgca gccaccgccg cgaagagaaa cagcactcct
     9541 gcgagtaaaa aatcagaacc ttccatcagc agcctcctgg agacagcgga tttgccagcc
     9601 agtcaccgta ggctctggcg tggctcgcca gctcctgtgc gctttgccgt ctcgcccagt
     9661 aaataatgat ggggcttaac caatgcatcc ggcacatgcc cgccgccagt tcaaaggggc
     9721 gcagaacatc gctcatcgga tagcgattca acgcgtcata acggtaagca ctttccggct
     9781 ccccggtggt aatcacgcta cgccagtact ttcccgccag ttggtttccc cccggtccgc
     9841 tggcaaaacc acggcttaat acccggtcca gccactcttt cagtagcgcc gggcagctat
     9901 aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat gcctgctcac
     9961 gagggatatc aataaaaaag tcgggatagt gcgcgtaaag gtcgtgcacg gtaacattgc
    10021 tgagctgcgt ggccggttta agcagaaccc ggtttgccac cgagtcctga gattccggat
    10081 gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg gtatggtttt
    10141 tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg cccatacatt
    10201 acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg attgtttttt
    10261 cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc gccaccatca
    10321 accctgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct accctgctgg
    10381 cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg gtaagctggc
    10441 aactggcgtg ggtgaatcag gaaacaccgg cgttaccgca agcggcgctg gaatatgtca
    10501 ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct acacgacgcc aacgaacgta
    10561 acgacgggca cgccattgcg accattcatg gcaagctgga tgctattgac gcatggagta
    10621 ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa caactggagc
    10681 gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc caggcgctga
    10741 tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat ctcgatgccg
    10801 ttatctggct ggaaaaatgg ctgaagagct atcagggcac gctgatcctg atctctcacg
    10861 atcgcgattt cctcgatccg atcgttgata aaattattca tatcgaacaa caaagcatgt
    10921 tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt ctggcgcagc
    10981 aacaagcgat gtatgaaagc cagcaggaac gcgtagcgca tctgcaaagt tatatcgacc
    11041 gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgcattaag atgcttgagc
    11101 gtatggagct gattgccccc gcgcacgtcg acaacccgtt ccgctttagc ttccgcgcgc
    11161 cggaaagcct gccaaatccg ttactgaaga tggaaaaagt cagcgcaggc tatggcgatc
    11221 gcattattct cgactcgatt aaactgaatc tggtccccgg ctcgcgcatt ggtctgctag
    11281 gccgcaacgg cgcgggtaaa tcgacattaa tcaaactgtt agccggcgaa cttgcgccag
    11341 tcagcggtga aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc cagcatcaac
    11401 tggaatacct gcgcgccgac gaatcgccta ttcaacatct ggcacgttta gcgccgcagg
    11461 agctggagca aaaactgcgt gactacctcg gcggctttgg tttccagggc gataaagtaa
    11521 ccgaagaaac gcgccgcttc tccggtgggg aaaaagcccg cctggtgctg gcattaattg
    11581 tctggcagcg tccgaatctg ctgctgctcg acgaaccgac caaccacctt gacctcgaca
    11641 tgcgtcaggc actcaccgaa gcattaatcg agttcgaagg cgcgctggtt gtcgtctcgc
    11701 acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac gatcgtaaag
    11761 tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac gtacaaaagc
    11821 aggaaaacca gaccgacgaa gcgccaaaag agaacgcgaa cagcgcccag gcacgtaaag
    11881 atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca gccactgcgt aaagagattg
    11941 cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg gaagagaaac
    12001 tcggcgacag cgaactgtac gatcaaagcc gtaaagcgga gttgaccgcc tgcctgcaac
    12061 agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcatggctg gaagcccagg
    12121 agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga taacaacgac
    12181 cgatgccaat gaatttagca gcagtgctaa attcacccct atgcgcggct ttagcaattg
    12241 tcatctacaa accatgctgc cgcgtctgtt ccgtcgcaag gtgaaattca ccccgtactg
    12301 gcagcggctg gagttgcccg acggcgattt tgtcgatctc gcgtggagtg aagatcctgc
    12361 acaggcgcgg cataaaccgc gtttagtggt atttcacggg ctggaaggca gtctcaatag
    12421 cccttacgct cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg gcgtggtgat
    12481 gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc attcgggcga
    12541 aaccgaagac gccagttggt ttttacgctg gctgcaacgc gagtttggtc atgcgccaac
    12601 ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg caaaagaagg
    12661 caatgatctc ccgattgatg cggcagtgat tgtctctgcg ccgtttatgc tggaggcctg
    12721 tagttaccat acggaaaagg gcttttcccg catttatcag cgttacttgc tgaacctgtt
    12781 aaaagccaat gccgcgcgca agctggcagc ctaccccgga acactgccga ttaatctcac
    12841 gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctcatcaccg ccagaattca
    12901 cggctacgct gacgctatcg actattatcg tcagtgtagc gccatgccga tgctcaatcg
    12961 gatagccaaa ccgacactga ttattcacgc caaagacgat ccgtttatgg atcatcaggt
    13021 gatcccgaaa ccggaaagtc tccccccgca ggtggagtat cagctgactg aacatggcgg
    13081 tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg agtcacgcat
    13141 tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg gcaagacctc
    13201 tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg caccgattat
    13261 ggtgaacatg agcgtacact tgaacagaaa gtctccgacg tcaaacgcca gctacagtgc
    13321 ggagaagcgg tgctggtatg gtcggagctg cacgaaacgg tcaatatcat gccgcgcagt
    13381 cagtttcgcg aataacctgc aaccgtcggc ggacgagata ataatgatag ctaagtcatg
    13441 gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc ggcgcgggga
    13501 ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg catgcagccg
    13561 aggtggaagg cgacagtttt caccgttata cccgcccgga aatggacatg gcgatccgca
    13621 aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac gacttcggcc
    13681 ttctggaaca aaccttcatt gaatacggtc agagtggcaa agggaaatct cgcaaatatc
    13741 tgcataccta cgatgaagcc gtaccgtgga atcaggtacc ggggacattc accccctggc
    13801 aatctttacc ggaacccact gatgtactgt tttatgaagg tttacacggc ggcgtagtca
    13861 cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtggtg cctatcgtta
    13921 accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac tcacgagaag
    13981 cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc acaccgcagt
    14041 tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacctca aacccgttcg
    14101 cggcaaaagg tatcccgtcg ctggatgaaa gctttgtggt gatccatttt cgtaatctgg
    14161 aagggatcga tttcccctgg ctgctggcga tgttgcaagg ctcattcatt tcccacatca
    14221 atacgttagt ggtgcctggc ggcaaaatgg gtctggcaat ggaattaatt atgctgccgc
    14281 tggtgcaacg attgatggaa ggaaagaaaa tcgagtaacg ctgctattac gccggataaa
    14341 atactatccg gcttcacaac gggatagtta aatcacgcgg caaccacttc atacgagtga
    14401 gtaatattca ccgctttttc cagcatcagc gccactgaac aatatttctc ggcagagaga
    14461 tcaaccgcac gcgcaaccgc tgcgtctttc aggtcacgac cggtgacgat aaaatgcaga
    14521 ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgct cagaggtcaa ttttacttca
    14581 caatcgacca catcctgacg ccctttttgc aggatagaaa ccacatcgat ggcactgcaa
    14641 ccacccgccg ccatcaacac catttccatc gggcttggtg ctttatcgcc tgagttgccg
    14701 tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc ttcgacccac
    14761 ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca gagtacgcgt
    14821 actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc tgaagcgaga caccaggaga
    14881 cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatatatt gatgtactgc
    14941 atgtatgcaa aggacgccac attaccgtgc agtacagttg atagcccctt cccaggtagc
    15001 gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga gaaagcttat
    15061 aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac tctcgaatgg
    15121 ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat tcaccagggt
    15181 gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct gatcaaagac
    15241 gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat tggcgaactg
    15301 ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac cgcctgtgaa
    15361 gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc ggacattctg
    15421 atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa agtgggcaac
    15481 ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaatct ggcaaaacaa
    15541 ccggatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca ggaaatcggt
    15601 cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct ggaagatcag
    15661 aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta atcccgtcag
    15721 agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc agactgattt
    15781 atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg cccgtggccg
    15841 ttgggttaat gcttggcgaa ttacgattcg gcctgctctt ctccctcgtt cctgcctgtt
    15901 gcaatattgc gggccttgat acgcctcata aacgtttttt caaacgctta atcattggtg
    15961 cgtcgctgtt tgccacctgt agcttgctga cacaggtgct actggcaaaa gatgttcccc
    16021 tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgaa ctggggccat
    16081 tgcacgcaaa attgcttccc gcatcgctgc tcgctgccat ttttaccctc agtctggcgg
    16141 gatacatgcc ggtctgggaa ccgttgctca tctatgcgct gggcactttg tggtacggat
    16201 tgtttaactg gttttggttc tggatctggc gcgaacaacc gctacgcgag tcactaagtc
    16261 tgctgtaccg tgaactggca gattactgtg aagccaaata cagcctgctt acccagcaca
    16321 ccgaccctga taaagcgctg ccgccgctgc tggtgcgcca gcaaaaagcg gtcgatctaa
    16381 ttacccagtg ctatcagcaa atgcatatgc tttccgcgca aaataatacc gattacaagc
    16441 ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg gtcagtttgc
    16501 atcagccgga agaggtgcaa aagctggtcg agcgtagcca tgcggaagaa gttatccgct
    16561 ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt ctttaccatc
    16621 gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa atcgcccgcc
    16681 aacacccgga taatccggtt gggcaattct gctactggca tttcagccgc attgctcgtg
    16741 tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa cagcggcgaa
    16801 tgccattact tccggcgctg aaaagttatc tgtcactaaa gtctccagcg ctacgcaatg
    16861 ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgcg cttcatctgc
    16921 cgaagtcgta ctggatcctg atgacagtat tgctggtgac gcaaaatggc tatggcgcaa
    16981 cccgtctgag gattgtaaat cgctccgtgg gaaccgtggt cgggttaatc attgcaggcg
    17041 tgacgctaca ctttaaaatt cccgaaggtt acaccctgac cttgatgctg attaccaccc
    17101 tcgccagcta cctgatattg cgcaaaaact acggctgggc gacggtcggt tttactatta
    17161 ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc cttccgcgtc
    17221 ttatcgatac cattattggt tgtttaattg ctttcggcgg tactgtctgg ctgtggccgc
    17281 agtggcagag cgggttattg cgtaagaacg cccatgacgc tttagaagcc tatcaggacg
    17341 cgattcgctt gattctgagc gaggacccgc aacctacgcc actggcctgg cagcgaatgc
    17401 gggtaaatca ggcgcataac actctgtata actcattgaa tcaggcgatg caggaaccgg
    17461 cgtttaacag ccattatctg gcagatatga aactgtgggt aacgcacagc cagtttattg
    17521 ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg ccaccggaac
    17581 tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag cagcgactgg
    17641 agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg gagatgcagc
    17701 cgcacgaagg cgcggcaggt acgctggagc agcatttaca acgggttatt ggtcatctga
    17761 acaccatgca caccatttcg tcgatggcat ggcggcagcg accgcatcac gggatttggc
    17821 tgagtcgcaa gttgcgggat tcgaaggcgt aatgcaggct aaaccatcgg atacagcgtt
    17881 cacgccgcat ccgacatctt ttgcctgatg cgtcttattt acccccaatc accttcgcca
    17941 tcgcgtgggc gaaacgatgc atcccttcat cgatatccgc atcttccacc accagcgacg
    18001 gcgcaaaacg catcacatca ggcccggcat tcagcaccat tacgccttcc tctgcacccg
    18061 catacaggaa atcacgcgcc tgacctttgt actgtggttt cagctctgcg ccaatcaaca
    18121 gccccatacc gcgaatatcg ctaaacacat cgtactgctg atcgatcttc tgcaaatggt
    18181 caacaaaacg ctggcgtttc gcctgaatgc cttccagcac ttcaggggta ttgatgatat
    18241 caaacgccgc gcccgctact gcacaggcca gaggattacc gccgtaggtg gaaccgtgag
    18301 aaccaggatg aaacgcggaa gcaatctccg ccgtggtcag catggcgcta atcgggaaac
    18361 cgccacctaa cgctttcgca gaggtcagaa tatccggcgt cacgccgtag tgcatatagg
    18421 caaacagatc gccggtccgc cccatcccgc actgcacttc atcaaacacc aataatgcct
    18481 gatgttgatc gcacagctcg cgcaagccct gcaaaaactc tggcgtcgct gccgtcacgc
    18541 cgccctcgcc ctgaatcggc tcaaccacca ccgcacaggt atggtcatcc atcacggctt
    18601 ttactgcgtg aagatcgtta aaaggcacgt ggatgatgtc tgccggtttt ggcccaaagc
    18661 cgtcggaata ttttggctgc ccacccaccg aaacggtaaa cagcgagcga ccatgaaaag
    18721 cgttatggaa ggcaataatt ttggttttga acgggctatg acgcacacag gcgtaatggc
    18781 gtgccagttt aaaggcggtt tcgttagctt ccgtgccgga gttcataaac accacgcgtt
    18841 cggcaaacgt tgcctcaatc agtttacgac caagacgcag cgccggttca ttggtgaaaa
    18901 cgttactgat atgccacaga gtttcgccct gggtttttaa cgcgttcacc agcgcaggat
    18961 ggcaatggcc caacgccgta actgcaatgc cacccgcgaa atcgacatac tccttgcctt
    19021 gctgatccca gattcggctc ccctgacctt ttaccggaat aaactctgcc ggagcataaa
    19081 tcggcagaat cacttcatcg aaagtcgcgc gtgtaattgc tgtttgttca attgccatct
    19141 catgatcacc ctgttacgca taaataaata tgaaattata atcacaaaat atgcataaaa
    19201 aatcactgaa tggcaatcag aaatcagcga tgcagaaaat tagccagcag ttgatgtcct
    19261 tgttcgctaa gaatactttc tggatggaat tgcacacctt ccagatccca ctggcgatgg
    19321 cgaatcccca taatctcgcg ggtttcgctc caggccgtca cttcaaagca ctccggtaat
    19381 gagtcaggtt ccaccaccag cgaatggtag cgtgtcacgg tgagtggatt tgccagcccc
    19441 ttaaatacgc ccacaccatt atgtgtaatc ggcgaggttt tgccgtgcat gacctttgcg
    19501 gcgcgcacaa ctttgccgcc aaatgcctgc gccattgcct gatgaccgag gcagacgcca
    19561 agaatcggca agcgtccggc atagtgacga ataacgtcaa gggagatccc ggcttcatct
    19621 ggcgtacagg ggccgggtga gatgacaatt ttttgtggtt taagggcgtc gatatccgcc
    19681 aacgtcaacg catcgttgcg cttaaccaac acatccgccc ccagttcaca aaagtactgg
    19741 tagaggttcc aggtaaaaga atcgtagtta tcaataagca ggatcatggc ggctccggta
    19801 cgaaagaacc gcgctatttt actcagattc cccggcttcg cttaccactt tgctaaatat
    19861 catctgcagc gcggtgagat cccccattgc cccattctga tttgcctgat tccaggcctc
    19921 tttttcgata ccctgccagc tcagttgata tccggcatga atcgccagtt gctcgaagaa
    19981 gatccgctgt gccaaaccac ttcccacccg gaagggatgc agcacgttga tttcacaata
    20041 gtaatgcgcc agccgctcga cgaacttcgc tttctccagg ccaaccagat aaccttcttc
    20101 ctccagatcc tgcatcaggg cattgccctc tttttcgata taagcaaagt ggcagaacgg
    20161 cgtatcgccc tgataaatat cgacttcgcg cagttgcccc gcccagtcga aaatatcctg
    20221 atacagctgg cgatggatag tgcgcaaatg cggtaaaccc cgaaccaacg ggccaagttc
    20281 aatggtcgca gcacgcagcg ccgtcatttc gtaagcggcc tgttcaagcc gctgctgctg
    20341 gcggatgttc agccggttac gcatgatatc aaggcctgga taaagatacg gatcgcgccc
    20401 ttcgccgaat ttatcgctca tagtgcctcc tcagcgcttc aagacgcgct aaagcctctg
    20461 cggcagtaag agtgactaaa ggcatctcga cgccttcaag acggcgactg gcctggaaat
    20521 tacgattacg ctgaagctcc cagagacggg acttttgctt atcggtgagt ttcttcactt
    20581 gatgcctccc tgaacgtgtc catttgccac aagtataagc ggcaaatccg ggttacgccg
    20641 ggagagcaga agcgcgggca agattgcccg cgcgagaaat cattacggca ggactttagc
    20701 ggaaaggata actaccggtt ttgacggcac attctggtac ggaccaacgt catgagtcgg
    20761 cacctgggaa atcttatcgg caacgtccat gcctttcacc actttaccaa ataccgcgta
    20821 accgaaatca cgctgaccgt ggtcaaggaa ggcgttatcg gcaacgttga taaagaactg
    20881 gctggtggcg ctgtctttgt cagcggtacg cgccatcgcg atggtgccac gcgtgttgcg
    20941 caggccgtta tcagcttcat ttttgattgg cgggtttggt tttttctgct gcatctgctc
    21001 ggtgaaaccg ccgccctgaa tcataaagcc aggaatgacg cggtgaaagg tcgtgttgtt
    21061 ataaaaacca ctgttcacat aatcgacaaa gttttgcaca gacactggcg ctttttgttt
    21121 atccagctcc agttcgatgt taccagctga ggttgtcaac aatacgtgcg ggtccccttt
    21181 cgctgccatt gctgcgggag aaagagcaga aagggcgaaa acagcagcca tcgccgccag
    21241 ggtcgatttg aacatgagat ttccttaaca aacagagcag aaaaaaagca agtgaagaga
    21301 ttctaaatag ccttcaagaa caaggccatc cctttaccta attttacgta tctgaaatat
    21361 atgtaacctg aggataactt tttattccgt gaccaaaatc acctcttaaa atgcaattta
    21421 gcaaccgatt gcaataaaac atttaatcag atcacaaaat cgcctaaaat cgcccgctcg
    21481 cggcatagcc cgctatgcca ttttattttt gaacgcaggc caatcatgac taacagcaat
    21541 cgcatcaagc tcacatggat tagctttctt tcctacgcac tgaccggtgc gttggttatt
    21601 gtcaccggga tggtgatggg aaatatcgcc gattatttca atctgcctgt ttccagcatg
    21661 agtaacacct tcaccttcct caatgccggc attttaatct ctatcttcct caacgcctgg
    21721 ctgatggaaa tcgtcccgtt gaaaacgcag ttacgttttg gctttctcct gatggtgctg
    21781 gcggttgccg gtttgatgtt cagccacagc ctggcgctgt tctcggcggc gatgttcatt
    21841 ctcggggtgg tcagcggcat caccatgtcg attggtacat tcctggtaac acaaatgtat
    21901 gaaggacgtc agcgcggttc acgcctgtta tttaccgact ccttcttcag tatggccggg
    21961 atgattttcc caatgatcgc cgcgtttctg ctggcacgta gcattgagtg gtactgggtt
    22021 tatgcctgca tcgggctggt atatgtcgct atttttattc tgaccttcgg ctgtgagttc
    22081 ccggcgctgg gcaaacatgc gccaaaaacg gatgctccgg tagagaaaga aaagtggggg
    22141 atcggcgtac tgtttctctc tgttgcggcg ctgtgctaca tcctcggtca gttaggtttt
    22201 atctcctggg tgccagaata tgccaaaggt ctgggtatga gcctgaatga cgctggcacg
    22261 ctggtaagta acttctggat gtcgtacatg gtcggcatgt gggcgttcag ctttattctt
    22321 cgcttctttg atttgcagcg cattctgacc gtactggctg gtctggctgc gattctgatg
    22381 tacgtcttta acaccggaac accggcacat atggcgtggt caattctcgc cctgggcttc
    22441 ttctccagcg cgatctatac caccatcatc accctgggtt cacagcagac caaagtaccg
    22501 tcgccaaaac tggttaactt tgtcctgacc tgcgggacca tcggtactat gttgaccttt
    22561 gtggttaccg gcccgattgt tgaacatagt ggtccgcagg cggcgctgct gacggcaaac
    22621 ggtctgtacg ctgtcgtctt tgtgatgtgc ttcctgttag gtttcgtcag ccgtcaccgt
    22681 cagcataaca ccctgacctc tcattaattg ttcatgccgg gcggcactat cgtcgtccgg
    22741 ccttttcctc tcttactctg ctacgtacat ctatttctat aaatccgttc aatttgtctg
    22801 ttttttgcac aaacatgaaa tatcagacaa ttccgtgact taagaaaatt tatacaaatc
    22861 agcaatatac cccttaagga gtatataaag gtgaatttga tttacatcaa taagcggggt
    22921 tgctgaatcg ttaaggtagg cggtaataga aaagaaatcg aggcaaaaat gagcaaagtc
    22981 agactcgcaa ttatcggtaa cggtatggtc ggccatcgct ttatcgaaga tcttcttgat
    23041 aaatctgatg cggccaactt tgatattacc gttttctgtg aagaaccgcg catcgcttat
    23101 gaccgcgtac acctctcgtc ttacttctct caccacaccg ccgaagagct gtcgctggtg
    23161 cgcgaaggct tctacgagaa acacggcatc aaagttctgg tcggcgaacg cgctatcacc
    23221 atcaaccgtc aggagaaggt gattcactcc agcgccggac gtaccgtttt ttatgacaag
    23281 ctgatcatgg cgaccggttc ctacccgtgg atcccgccaa ttaaaggttc tgatactcag
    23341 gactgctttg tctatcgcac cattgaagac ctcaacgcca ttgaatcctg cgctcgtcgc
    23401 agcaagcgcg gtgccgttgt tggtggcggc ctgttaggtc tggaagctgc tggcgcgctg
    23461 aaaaacttag gcattgaaac ccacgttatc gaatttgccc ctatgctgat ggcggaacag
    23521 cttgatcaga tgggcggcga gcagctgcgt cgcaaaatcg aaagtatggg cgtgcgcgtt
    23581 cacaccagca aaaacactct tgagattgtg caggaaggtg ttgaagcgcg taaaaccatg
    23641 cgttttgccg acggcagcga actggaagtc gactttatcg tcttctctac cggtatccgt
    23701 ccgcgcgata agctggcaac ccagtgtggt ctggacgttg ctccgcgtgg gggtatcgtc
    23761 ataaatgatt cctgccagac ttccgatccg gatatctacg ccatcggtga atgcgcaagc
    23821 tggaacaacc gtgtatttgg tctggtagca ccaggctaca aaatggcgca ggtcgccgtt
    23881 gaccatattc ttggtagcga aaacgccttt gaaggtgcag accttagcgc caagctgaaa
    23941 ctgctgggcg tagacgtagg cggtattggt gatgcgcacg gtcgcacgcc tggcgcacgt
    24001 agctacgttt acctcgacga aagcaaagag atctacaaac gcctgattgt cagcgaagac
    24061 aacaaaaccc tgctcggtgc ggtactggtg ggcgatacca gcgactacgg caacctgctg
    24121 caactggtgc tgaacgctat cgagctgccg gaaaacccgg attccctgat cctgccagcg
    24181 cactcgggta gcggtaagcc gtctatcggt gttgataaac tgccggacag cgcgcaaatc
    24241 tgctcctgct tcgacgtcac caaaggcgac ctgattgctg ctatcaacaa aggctgccac
    24301 accgttgcgg cgctgaaagc cgaaaccaaa gccggtacgg gctgcggcgg ttgtattccg
    24361 ctggtcactc aggtactgaa cgcggaactg gcgaaacagg gtatcgaagt taacaacaac
    24421 ctgtgtgaac actttgctta ttcgcgtcag gaactgttcc atttgatccg cgttgaaggc
    24481 attaaaacct tcgaagaact gctggcgaaa cacggcaaag gttacggttg tgaagtttgt
    24541 aaaccgaccg tcggttcgct gttggcctcc tgctggaacg aatacattct gaagccggaa
    24601 catactccgc tgcaggattc taacgacaac ttcctcgcga acatccagaa agacggcacc
    24661 tactcggtga tcccgcgttc tccgggcggt gaaatcaccc cggaagggct gatggcagtg
    24721 ggtcgtatcg cgcgtgaatt taatctctac accaaaatca ccggctctca gcgcctggcg
    24781 atgtttggcg cacagaaaga cgatctgcca gagatctggc gtcaactgat tgaagccggc
    24841 ttcgaaaccg gtcatgccta tgcgaaagca ctgcgtatgg cgaaaacctg cgtgggaagc
    24901 acctggtgcc gctacggtgt tggcgacagc gtcggcctcg gcgtggaact ggaaaaccgt
    24961 tacaaaggca tccgtacgcc gcacaaaatg aagttcggtg tctccggctg tacccgtgaa
    25021 tgttcagaag ctcagggtaa agacgtgggt attatcgcca ccgaaaaagg ctggaacctg
    25081 tacgtttgcg gtaacggcgg catgaaaccg cgtcatgcgg atctgctggc agcggatatc
    25141 gatcgcgaaa cgctgatcaa atatctcgac cgcttcatga tgttctacat ccgtactgcc
    25201 gacaaactga cgcgtaccgc gccgtggtta gaaaacctcg aaggtggcat cgattacctg
    25261 aaagcggtga tcattgacga caaactgggg ctgaacgctc atctggaaga agagatggcg
    25321 cgcctgcggg aagcggtagt gtgcgaatgg actgaaacgg tcaatacgcc gtctgcgcag
    25381 actcgcttca aacacttcat caacagcgac aagcgtgacc cgaacgtgca gatggtgcca
    25441 gagcgcgaac agcaccgtcc ggcaacgccg tatgaacgta tcccggtaac tctggtggag
    25501 gacaacgcat gagccagtgg aaagacatct gcaaaatcga tgacatcctg cctgaaaccg
    25561 gcgtctgcgc gctgttaggt gacgaacaag tagcaatttt ccgcccgtat cacagcgatc
    25621 aggtgtttgc gatcagcaac atcgacccgt tcttcgagtc cagcgtgctg tcacgcgggt
    25681 tgattgcgga acaccagggc gagctgtggg tcgccagtcc gctgaaaaag cagcgttttc
    25741 gcttaagcga cggcttgtgc atggaagacg aacagttttc cgtcaagcat tacgacgcgc
    25801 gagtgaaaga cggcgtggta cagctgcgcg gttaatattt taacgggagg cgcaatgcct
    25861 cccctttttg catggtcctg taataacctt
//
LOCUS       DADTFG010000063        25528 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.063, whole genome shotgun sequence.
ACCESSION   DADTFG010000063 DADTFG010000000
VERSION     DADTFG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.063"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            32..376
                     /locus_tag="J1138_002388"
     CDS             32..376
                     /locus_tag="J1138_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454176.1"
                     /translation="MLSACARTAPVQQISTTVSVGHTQEQVKNAILKAGAQRKWIMTQ
                     VSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDGKIHKNYNRWVRNLDK
                     DIQVNLSTGATL"
     gene            441..1499
                     /locus_tag="J1138_002389"
     CDS             441..1499
                     /locus_tag="J1138_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA8454177.1"
                     /translation="MYEQDTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARDSWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKLCIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVT
                     ERHDNLGVGHTLLICQKK"
     gene            1540..2262
                     /locus_tag="J1138_002390"
     CDS             1540..2262
                     /locus_tag="J1138_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HBA8454178.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSILFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            2259..3080
                     /locus_tag="J1138_002391"
     CDS             2259..3080
                     /locus_tag="J1138_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBA8454179.1"
                     /translation="MSKLMSRLGWAWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVRGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            3055..3312
                     /locus_tag="J1138_002392"
     CDS             3055..3312
                     /locus_tag="J1138_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA8454180.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            3324..3575
                     /locus_tag="J1138_002393"
     CDS             3324..3575
                     /locus_tag="J1138_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA8454181.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            3580..4161
                     /locus_tag="J1138_002394"
     CDS             3580..4161
                     /locus_tag="J1138_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HBA8454182.1"
                     /translation="MSGIRSLPMIKLLTGLLLLVWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTYQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRRKMIKRKTP"
     gene            4158..5516
                     /locus_tag="J1138_002395"
     CDS             4158..5516
                     /locus_tag="J1138_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBA8454183.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNSMNKRVYAQLQELFHD
                     TP"
     gene            5503..5856
                     /locus_tag="J1138_002396"
     CDS             5503..5856
                     /locus_tag="J1138_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HBA8454184.1"
                     /translation="MIRHEIERHLVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            5847..7523
                     /locus_tag="J1138_002397"
     CDS             5847..7523
                     /locus_tag="J1138_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA8454185.1"
                     /translation="MSVNFYPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVSEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKRATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMCHSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FTALLYPVVGVYWLTAARARKASQDWLARVRQQQPQAAKINSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIEGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            7527..7949
                     /locus_tag="J1138_002398"
     CDS             7527..7949
                     /locus_tag="J1138_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBA8454186.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            7946..8551
                     /locus_tag="J1138_002399"
     CDS             7946..8551
                     /locus_tag="J1138_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HBA8454187.1"
                     /translation="MKFLPLLALLISPFVSALTRDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            8520..10838
                     /locus_tag="J1138_002400"
     CDS             8520..10838
                     /locus_tag="J1138_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HBA8454188.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKTLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIASYYPCGIAWVDRKSSF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10835..11419
                     /locus_tag="J1138_002401"
     CDS             10835..11419
                     /locus_tag="J1138_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HBA8454189.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPIRIEQHVFKYHITIQYLGD"
     gene            11421..12590
                     /locus_tag="J1138_002402"
     CDS             11421..12590
                     /locus_tag="J1138_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HBA8454190.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFSRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            12587..13051
                     /locus_tag="J1138_002403"
     CDS             12587..13051
                     /locus_tag="J1138_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HBA8454191.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            13051..13782
                     /gene="fabG"
                     /locus_tag="J1138_002404"
     CDS             13051..13782
                     /gene="fabG"
                     /locus_tag="J1138_002404"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBA8454192.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13779..15008
                     /locus_tag="J1138_002405"
     CDS             13779..15008
                     /locus_tag="J1138_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HBA8454193.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(15848..16879)
                     /locus_tag="J1138_002406"
     CDS             complement(15848..16879)
                     /locus_tag="J1138_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA8454194.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            17150..17593
                     /locus_tag="J1138_002407"
     CDS             17150..17593
                     /locus_tag="J1138_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA8454195.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            17609..17896
                     /locus_tag="J1138_002408"
     CDS             17609..17896
                     /locus_tag="J1138_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA8454196.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            17909..19165
                     /locus_tag="J1138_002409"
     CDS             17909..19165
                     /locus_tag="J1138_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBA8454197.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFASADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(19381..19665)
                     /locus_tag="J1138_002410"
     CDS             complement(19381..19665)
                     /locus_tag="J1138_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA8454198.1"
                     /translation="MEQKTLSAEPRRLFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(20086..21099)
                     /locus_tag="J1138_002411"
     CDS             complement(20086..21099)
                     /locus_tag="J1138_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 domain-containing protein"
                     /protein_id="HBA8454199.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(21111..22427)
                     /gene="fucP"
                     /locus_tag="J1138_002412"
     CDS             complement(21111..22427)
                     /gene="fucP"
                     /locus_tag="J1138_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBA8454200.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(22455..23375)
                     /gene="rbsK"
                     /locus_tag="J1138_002413"
     CDS             complement(22455..23375)
                     /gene="rbsK"
                     /locus_tag="J1138_002413"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBA8454201.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            23678..24460
                     /locus_tag="J1138_002414"
     CDS             23678..24460
                     /locus_tag="J1138_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBA8454202.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(24462..24560)
                     /locus_tag="J1138_002415"
     CDS             complement(24462..24560)
                     /locus_tag="J1138_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HBA8454203.1"
                     /translation="MISQIDKLEYVMKVQRNQSNPTMFNKSTVFFQ"
BASE COUNT         6248 a         6511 c         6410 g         6359 t
ORIGIN      
        1 aaaatggttt gccatcacaa tttttatcag tatgttgagt gcctgtgccc gtacggcccc
       61 agtgcaacag ataagcacca ctgtcagtgt gggtcatact caggagcagg ttaaaaatgc
      121 cattttgaaa gcaggtgcgc agcgcaagtg gattatgacg caagtgtccc ctggagttat
      181 taaagctcgc tatcaaacac gaaatcacgt tgcagaggtt cgtattacat atacagctac
      241 ctactataac atcaaatatg acagtagcct gaatctgcag gcttctgatg gaaaaattca
      301 taaaaactat aaccgctggg tgcgtaacct agataaagat atacaggtta acttatctac
      361 aggagcaacg ttatagtgat gctgatatcg ggctggaatg gcccgatagt atttaacttt
      421 ctctataaga atggttatat atgtacgaac aggacacgct cagcgcactg gatgccatca
      481 ccgaagcaca gcggattgcc tttgctccta tgcttttcca ggcagctttg tgcctgcgca
      541 atgcaggaat attagattat ttggatcaac agggtaaaca gggagctccc cttaatgcga
      601 ttactgagca cacagtcctg aatgaatatg ccgtgggtgt gttactggat atgggcttaa
      661 gcgggcgaat tattacctgt aaagagggaa tttattacct ggcgaaaatc ggtcattacc
      721 tcctgcatga cactatgact cgcgtgaata tggatttcac ccaggatgtc tgttaccagg
      781 ggctcttctt ccttgccgat tcactcaatg aaggaaaacc ttccgggtta aaagtatttg
      841 gcgactggcc gacgatatat ccggcacttt cgcaattacc agacgccgca cgtgatagct
      901 ggtttgcgtt tgatcattac tattcagatg gcgcatttaa cgccgcactg ccttatgtat
      961 tcgccaacaa ccccacaacc ttgtacgatg tgggtggcaa tacgggtaag tgggctttgc
     1021 gctgctgcaa attcaatgaa aacatcgccg ttacgttatt agatctgcca caacaaatcg
     1081 tactggcgaa agaaaatatc gcgaatgcag gtttttctga tcgcatagat ttccatgcgg
     1141 tggatatgct aagcgatgcc ccattaccag gcgaggctga tatctggtgg atgagccaat
     1201 ttttagactg tttttcaccc gaacaaataa ttagcatact cagcaaaatt gccagcgtaa
     1261 tgaagcctgg cgcaaaactt tgcatcatgg agttgttctg ggatgcacag cggtttgaag
     1321 cgacatcgtt tagcctgaat gcatcctcac tttattttac ctgcatggca aacggtaaca
     1381 gccgtttcta tagtgcagag aaattttacg actatctgaa taaagcagga ttccaggtaa
     1441 cagagcgtca cgataattta ggcgttgggc atacactttt gatatgccaa aagaaataat
     1501 cgtcacttaa taattttggt ttaccgcggg cgcgcgttga tgaaatttgc attaaatatt
     1561 accaactggc aggcactggc gcctggactt agcgatgtgc agcaatggca agcatggtcg
     1621 cgccagccgt gggcgattga ccctgccgcg ccgttggcaa aattaagtga actgccaatg
     1681 atgaccgctc gtcgtctcag ttcaggcagc aaactggctg ttgaatgcgg tctgacaatg
     1741 ctacgccgcc atcagccaga cgctgttttg tataccagtc gtcatggcga actggagcgc
     1801 aattaccgca ttgttcatgc actggcaacc gaacaggcgc tttctccgac ggacttcgcg
     1861 ctttccgtac acaactcttc cgtgggtaac ctgaccattg tggccaaaca gccgatcgtt
     1921 tcgtcatcgc tttcggctgg tcgcgacagt ttccagcaag gtttgtgtga agtgcttagc
     1981 ctgctacaag cgggatacca gcgcgtgttg atggtcgatt ttgatggctt tctacctgag
     2041 ttttatcacc cgcaactgcc cgcagaaatg cctacctggc cgtacgccgt cgcgctggtg
     2101 attgaagctg gcgatgactg gcaatgtgaa acgcagccag ccattgcggt aaatgaaaca
     2161 acactgccgc aaagcatctt gtttttacag cactatttac aaaatgcaga tgctttttca
     2221 ctgcctggcg agcgcgtgca atggcgctgg agccgcagat gagtaagctg atgtcgcgcc
     2281 tgggatgggc atggcgactg gtcatgaccg ggttatgttt tgtcctgttt ggtctcggcg
     2341 gcctgctgct ttcagtcgtg tggtttaaca tattgttagt tttggtgtgg gatacttccc
     2401 gccgccgtcg tttggcacgt cgcagcattg ccgccagttt tcgcctgttt ttaactgtcg
     2461 cgaaagggct tggcgtgctg gattatcgta ttgatggcgc tgaaatttta cgtcaggaac
     2521 gcggttgcct cgtcgtagcc aatcatccca cgctgatcga ttacgtgctg ctggcatcag
     2581 tcatgccaga aactgactgc ctggtgaaaa gcgcgctgtt aaaaaatcct tttcttggcg
     2641 gcgtcgtgcg agcggcagat tatttaatca acagcgaagc agaaaccctg ttaccccgct
     2701 gccaacagcg actggcacag ggtgacacta tcctgatttt ccccgaaggc acacgaacga
     2761 agcccggtga aaaaatgacg ttacagcgcg gtgcagcgaa tattgccgtg cgctgcacca
     2821 gcgatttacg catcgtgacg atcggctgta gcgagcatct gctggataaa caaagtaagt
     2881 ggtatgacgt cccgcctgcc aggccgtttt ttacggttga agtccgtggg cgggtagaaa
     2941 tcaataaatt ttacgatgca acttcacaag agccgacgct ggctgcaaga cagttaaacc
     3001 ggcatctttt gcttcaatta cagcaaggtt gtttacctct atcaggaata aataatgcaa
     3061 gcgctttatc tggaaattaa aaacctcatc atcagcaccc tcaatctgga cgacttgact
     3121 cctgatgata ttgatatcaa tgcgccactg tttggcgacg gtttagggct ggactctatc
     3181 gatgcactgg aactgggact ggcagtaaaa aatgagtacg gcatcgtgct ctctgcggaa
     3241 agcgaagaaa tgcgtcagca ctttttctcc gtcgccactc tggcctcttt cattgctgcg
     3301 caacgtgcct gaggaaaacc atcatgacag accaacaaac catttatcag gaagtctcag
     3361 ccctgctggt taagctgttt gaaatcgatc cgcaggacat caaacctgaa gcgcgactgt
     3421 acgaagatct ggaactggac agcatcgacg ccgttgacat gattgtgcac ctgcaaaaga
     3481 aaaccggtaa gaaaatcaag ccggaagagt ttaaagcagt gcgtacagtc caggatgttg
     3541 tcgaggctgt agaacgcctg ctacaagaag gctaatttga tgtcgggcat tcgctcgcta
     3601 ccgatgatta agttgctgac cggattattg ctgctcgtct ggcctttttt aatctggcta
     3661 ggtttggtgc ataacagcct gcactggctg ttgccgctga tggcgctgtt gcttttattg
     3721 cgcctgcgcc agccccgtcg ccagacgggg ccgctccagg tcgtcacaaa aatcgttgct
     3781 gttgtgggca ttgtattgtg tgtctccagt tttctgttga aaacgtacca gttactgctg
     3841 ttttacccgg tagtggttaa cgcagtcatg ctggcggttt ttggcggttc gctgcggtcg
     3901 acaatgccga tagttgagcg gctggcacgt ttgcaggagc cagacctccc ggaaaaggcc
     3961 gtgcgatata cccgccgcgt aacgcaggtc tggtgcctgt tttttatttt caatggcagt
     4021 attgcgttat ttaccacctt gtatggcaac atgcccttgt ggacagcctg gaacggtatc
     4081 attgcttacc ttctgatagg tttcctgact gcaggggaat ggctgatacg tcgtaagatg
     4141 atcaaacgga aaacaccatg aatcagactc tctcactttc acagtggttg acagcctctc
     4201 gccctgtaac aactcctgtt gcctggcttg gggaatatac ctggacactg gggcatctgc
     4261 ggcatgatgt agcgcttttg atcgaccatt tacgcgacca gccaggcaat cgctgggcac
     4321 tttgttttga aaacagctat ctttttattg tagcgttgtt ggccacgcta cactcaggta
     4381 aaatcccggt aatcccgggc cataatcgcg ccgctttgtt aaatgtacaa cgctccttgt
     4441 ttgattgcgt cctgagtgat aaaaggctgg gctggaacgg tccacagttt gttgtaagca
     4501 catctcacca gatgacgacc agcacgatag cctttgatga tattgcttct gacgcgttta
     4561 ttgaactttt cacatcgggt tcaaccggac aaccaaaaca aattgcaaaa cctgttatca
     4621 gccttgatca agaagcaaac atgctggcaa ctcactttgc agaccgacta ctgggctgtc
     4681 gttttgtagc atccgtgatg cctcagcacc tgtatgggct aactttccgt atttttttac
     4741 ccatggcact tggcctgcca ttgcatgccg caatgctctg gtattctgag caacttgccg
     4801 cattaagcca tacctaccgc tatgcctttg tcagcagccc ggcgttcctt aagcgactcg
     4861 accttcatct gacgcctccc ccagtggaaa tgattctctc tgccggcggt atgctaccat
     4921 ggcaggatgt agagaaaact tccacctggc tgaatatatg gcccgatgaa atctatggta
     4981 gtaccgaaac aggtattctc gcatggcgtt accgtcaaca ggataatatc ccctggttta
     5041 ctttttctga tgttcatctt tcacaggaaa gtgagggggt tcgtgtcttt tcgccactaa
     5101 tccctgctga aggattggtg cttgacgata tgttgcaatt caatgaaaac ggtcagttcc
     5161 acctgatcgg acgacgtggt cgagtggtaa aaatagagga gaagcggata tcactggttg
     5221 aaatcgaaca acgacttctg gcacttgacg gtattatgga tgtcgctgca ataccattaa
     5281 tacgcaatgg ccgccaggct atcggtgtcg tggttgtacc aaataaagag gcccgtcaaa
     5341 tatggcaatg ctccggaggg aaaactctgg agttgtcctg gcgcaaagca ctattaccat
     5401 ggcttgaacc tgttgcagta cctcgttact ggcgcattat tgatgaaata ccagtaaaca
     5461 gtatgaacaa gcgtgtctat gcgcaattac aggagttatt tcatgatacg ccatgaaatt
     5521 gagcgccatc tggttcaacc gcaggagatg gaaatcgttc tataccttga cccgatgctc
     5581 tactggttta atggtcattt tgccgtacag cctctgttac caggcgtggc ccagttggac
     5641 tgggtaatgc attatgctac cacgctgctt gctccgggct ggcgttttcg tagtatccag
     5701 aatgttaaat ttctggcgcc acttatacct gagacgactg ttaccctgca attaacatgg
     5761 caggaaacaa gtcaggtact cactttctgc tatcagcgtc acgacggtga cgcccgccac
     5821 accgccagta gcgggaagat ccgtctatgt cggtaaactt ttatccctgc gtgttgatcc
     5881 cctgctacaa ccacggcgcg atgatgccgg gcgtgctggc gcgtcttaag ccatttaatc
     5941 tgccctgtat tgtggtggat gacggcagcg atgccgccac gcaacagcaa ctggataatc
     6001 tggttagcga acagcctggc gtgaccttaa ttcgcctggc agaaaacgca ggtaaaggcg
     6061 cagcagtgat gcgtggcttg caggcagcgg cagacgcagg gttcagccat gcggtgcagg
     6121 tggatgctga cggtcaacat gcgattgaag atatccccaa actgctggca ctcgctgaac
     6181 aacaccctgt ggcgctgatc tccggtcagc caatttacga tgactccatt ccccgctcac
     6241 ggctgtacgg gcgctgggtc acccacgtct gggtatggat cgaaacgctc tccctgcaac
     6301 tgaaagacag catgtgcggt tttcgcgttt atccggttgc gccgacgctg caactggcaa
     6361 aacgcgccac catcggcaag cggatggatt tcgacaccga agtgatggtg cgcctctact
     6421 ggcagggaaa taccagctat ttcgtgccga cccgcgtcac ctatccactg gacgggcttt
     6481 cgcattttga tgccctgaaa gataacgtcc gcatctcgct catgcacacg cgtctgtttt
     6541 tcggcatgtt gccgcgtatt ccttcactgc tgatgtgcca ctcttccagc cactgggcgc
     6601 ggcagagtga agttaaaggg ttatggggaa tgcgcctgat gctgctggtc tggcgtctgc
     6661 tgggaagaac ggcgtttacc gcgctgcttt acccggtggt tggcgtctac tggctcaccg
     6721 ctgctcgtgc gcgtaaagcg tcgcaagact ggctcgcccg tgtgcgccaa caacaaccac
     6781 aggcagcaaa aatcaacagc tatcagcact ttctacgttt cggcaacgcc atgctcgaca
     6841 aaatcgccag ctggcgcggc gagctgcaac tcgggcgtga tgtgctgttt gcgccaggcg
     6901 cagaagcggc gcttgacgtc cgcgatccgc gcggcaaatt gctgctggcc tcacatcttg
     6961 gcgatgtgga agtgtgccgg gcgctggcaa aaattgaggg ctacaaaacc attaacgcgc
     7021 tggtgtttag cgaaaacgcc caacgcttta aacagataat gcaggagatg gctccccagg
     7081 caggcattaa cctgatgccg gtaacagata tcggcccgga aaccgccatc ctgctgaaag
     7141 agaagctgga taacggcgaa tgggtggcga ttgtcggtga ccgcatcgcc gtcaccccgc
     7201 aacgcggcgg cgactggcgc gtctgctgga gtccgtttat gggccagcct gcgcccttcc
     7261 cacaggggcc gtttattctc gcctctattt tgcgctgccc ggtgaatctg attttcgccc
     7321 tacgccagca cggcaagctg catattcact gcgaaacctt tgccgacccg ctgctgttgc
     7381 cgcgtggcga acgccaacag gcgctgcaaa acgctatcga ccactacgcc gcgcgtctgg
     7441 aacattacgc gctccagtcg cccctcgact ggtttaattt tttcgatttc tggcaactgc
     7501 cggaaattca ggacaaggag taaagggtgc ttaacgatcc ccgatttacc gctgaagtcg
     7561 agctgaccat tccctttcac gacgtcgata tgatgggcgt ggcctggcac ggtaactatt
     7621 ttcgctactt tgaagtggcc cgcgaggcgc tgctcaacca gttcaattac ggctatcggc
     7681 agatgaaaga gtccggttat ttgtggccag tggtcgatgc gcgggtgaaa tatcgccacg
     7741 ccctcacctt tgagcaacgc atccgcgtgc gtgcgcatat cgaagagttc gaaaaccgtc
     7801 tgcggattgg ctatcagatt ttcgatgccg aaacgggcaa acgcgccacc accggataca
     7861 ccattcaggt cgcagtcgac gagcaaagcc gcgaactgtg ctttgtcagc cccgatattc
     7921 tgtttgaacg catgggagtc aaaccatgaa atttttaccg ctgctggcgc tgctgattag
     7981 cccgtttgtg agcgccctga cccgggacga tcttcagcaa cgctttaccg aacaaccggt
     8041 gatccgcgcc catttcgatc aaacccggac gattaaagat ctgccgcagc cgctgcgatc
     8101 tcagggtcag atgttgatcg cccgcgacca ggggttattg tgggatcaaa cctcaccgtt
     8161 ccccatgcaa ctattactgg acgataaacg catggtgcag gtgatcaacg gtcagccgcc
     8221 gcaaatcatc acggcagaaa acaacccgca gatgttccag tttaaccacc tgctgcgcgc
     8281 gctgttccag gccgatcgca aagtgctgga acaaaacttc cgcgtcgaat ttgccgacaa
     8341 aggcgaaggc cgctggacgc tgcgcctgac gccgaccacc acgccgctgg acaaaatttt
     8401 caacaccatc gatctcgccg ggcaaaccta tctggagagc attcagctta atgataaaca
     8461 gggcgatcgc accgatatcg ctcttaccca acatcaactg acaccagcgc aactgaccga
     8521 tgacgaacgc caacgctttg ccacccagta aacgccccgc gctgttatgg gggctagtct
     8581 gcctggtcat ggcggcggcg ttgctgatcc tgctgccaca atcacgactg aacagtagcg
     8641 tgctggctat gttacccaaa caggcgatgg gcgatattcc tccggcgctg aatgacggct
     8701 ttatgcagcg tctggaccgc caactggtgt ggctggtcag ccccggtaaa gaggctaatc
     8761 ccagggtggc tcaggagtgg ctgacgctgc tgcaaaaatc cgctgcgctc ggcgacatta
     8821 aagggccaat ggatgccgcc agccagcagg cgtggggagc gtttttctgg cagcaccgca
     8881 acggcctgat tgaccccgac acccgcgccc gcctgcaaaa tggcggcgaa gcgcaggcga
     8941 agtggatcct ctcccagctt tattctgcgt tctccggggt aagcggcaag gagctgcaaa
     9001 acgatccgct gatgttaatg cgcggctcgc agctggcgat ggcgaaaaat ggccagcgtt
     9061 tacgactaat ggacggctgg ctggtgacac aggatcccca gggcaattac tggtatctgc
     9121 tgcacggcga actggcggga tcgtcgtttg atatgcagca aacccaccag ctcatcacga
     9181 ccctgaatac gctggaaaag actctgaaaa cgcgttaccc gcaggcgcag ttgctctcac
     9241 gcggcacggt gttttacagc gattacgcca gccaacaggc gaagcaggat atctccaccc
     9301 tgggcgtggc aacgctgctg ggggtgatat tgctgattgt ggcggtgttc cgctctttgc
     9361 gcccgttgct gctttgcgtg atttccatcg gcatcggcgc gctggcggga acggccgcca
     9421 cattgttgat tttcggtgaa ttacacctga tgacgctggt gatgagcatg agcgttatcg
     9481 gcatttccgc tgactacacg ctctattatc tcaccgagcg gatggtgcac ggcaacgacg
     9541 tttcgccgtg gcaaagcctg gcgaaagtac gcaatgccct gctgctggcg ctgctcacaa
     9601 ccgtggcggc atatctgatt atgatgctcg cccccttccc cggcattcgc cagatggcga
     9661 tttttgccgc cgtcgggttg agcgcctcct gtctgaccgt cctgttctgg catccgtggc
     9721 tgtgccgtgg tctgccggtg cgcccggttc cggcgatggc gctgatgcta cgctggctgg
     9781 cagcgtggcg gcgcaataaa aaactgtcgc ggggtctgcc cgttgcgctg gcgctgtttt
     9841 cgctggcggg gatgtcaatg ctgcgcgtcg atgacgatat ctcgcagtta caggcgctgc
     9901 cgcagcacat tctggcgcag gagaaagcca ttaccgccct gaccggacaa agcgtcgatc
     9961 aaaaatggtt tgtagtttac ggcgactccc cacagcaaac attgcggcga ctggagaaat
    10021 ataccgcctc acttgagtat gcgaaaaaag aggggcttat cagcaactac cgcactattc
    10081 cgctgaactc cctggcgcgg caggaggaag acttagacct gctgaaaacg gctgccccga
    10141 cggtaacaaa agcactgcaa aatgcagggc tgacggcggt gaaaccagat ctcaacgcca
    10201 tgccggtgaa ggtcgatgaa tggctggcaa gccccgccag tgaaggctgg cgtctgctgt
    10261 ggctgacgct ggaaaacggc gaaagcggtg tactggtgcc ggttgaaggg gttaaaagta
    10321 gcgcattgtt gcaggaaatc gccagctatt acccttgcgg cattgcctgg gttgatcgca
    10381 aaagctcctt tgatgaattg ttcgcacttt accgctacgt cttaaccggc ttgttgctgg
    10441 tggcgctggc agtgattgcc tgcggcgcag tggcccgtct cggctggcgc aaagggctta
    10501 tcagcctggt gccttcggtg ctttcgctgg gctgtggtct ggcggtgctg gcgatgagcg
    10561 ggcaggcggt gaatctcttt tcgctgctgg cgctggtgct ggtgcttggc atcggtatca
    10621 actacacgct gtttttcagt aatccgcgcg gtacaccgtt aacttcgcta ctggcgatcg
    10681 cgctggcaat gctcaccacc ttgctgacgc tgggaatgtt ggtattcagc gccacccagg
    10741 ccatcagcag ttttggcatt gtgctggtga gcggtatttt caccgccttc ctgctttcgc
    10801 cgctggctat gcccgataaa aagagaacaa aaaaatgatc aaatccacat tctggcgagc
    10861 gttcgccctg accgctacgc ttatcctcac cggctgtagc cactcgcaac cagaacagga
    10921 aggccgcccg caggcgtggc tgcaacctgg cacgcgcatc acgctgccgg caccagggat
    10981 ttcacccgca gtcaattcgc agcaactgtt gaccggcagc ttcaacggca aaacccagtc
    11041 gctgttagtg atgcttaacg ccgacgatca gaaaatcacc cttgccgggc tgtcgtcggt
    11101 cggcattcgc ctgtttctgg tgacctacga tgcgcaaggg ctacgcgccg agcaatccat
    11161 cgtcgtccca cagttaccgc ccgcaagtca ggtactggct gacgtgatgc tcagccactg
    11221 gccgattagc gcctggcaac cgcaacttcc cgcaggatgg acacttcgcg acaacggtga
    11281 caaacgcgag ctgcgtaacg ccagcggcaa actggtcacg gaaatcacct atctgaatcg
    11341 ccagggaaaa cgcgtgccaa tcagaattga gcagcatgtc tttaaatacc acatcaccat
    11401 tcaatactta ggtgactgat atgatttata tttccgccgt cggcatgatc aacgccctgg
    11461 gaaataacct tgacgaaatc gcagccaacc tggctcgcgg tgtcgccccc ggcatgcgcc
    11521 cacgcgcagg ctggttgcag gggcatccgc aggcggtcct ggctggcgtg gatggcgagc
    11581 ttccgcttat cccggaaaaa ttcgctgcgc atcgctcgcg caacaatcag atcctgctgg
    11641 cggcgctggc gcagctccag ccgcaggttg atgacgctat cgcgaaatat ggctgcgagc
    11701 gcatcgcgat tgtgcttggc accagcacct ccggcctgca tgaaggcgac acgcacgtta
    11761 acctgcacac ccacgggcag ccgagcacca cgtggcacta tgcacaacaa gaactcggcg
    11821 atccgtcgcg ttttctcagc cactggctgg cgctcgacgg cccggcttat accctttcaa
    11881 ccgcctgctc ctccagtgcc agagcaatga tcagcgggcg caggcttatc gaagcggggc
    11941 tggtcgatgc cgccattgtg ggtggcgcag acaccctgag ccgaatgccg attaacggtt
    12001 ttcacagcct cgaatcgctg tcgccaacct tatgtcagcc gtttagtcgt gaccgcgcag
    12061 gtatcaccat tggtgaaggg gcggggctaa tgctgctgac ccgggaaccg cagcccatcg
    12121 cgttgctggg cgttggtgaa tcgagcgacg cttaccatat ttcagccccg catccgcagg
    12181 gcgaaggagc gatccgcgct attaatcagg cgctgaccga tgcgcagctt acgccggatg
    12241 acgtcggcta tatcaacctg cacggcaccg ccacacagct caacgaccag attgaatcaa
    12301 tggtggttaa cgccctgttt ggcgaacgcg taccgtgcag ctccaccaaa catttgaccg
    12361 gacacacgct cggcgcggct ggcattaccg aggcggcaat tagtatgttg attttacagc
    12421 gtgatttacc gctgcccgct caggacttca gcctgtcgcc gcgcgatccc acgctgccac
    12481 cgtgtggcat tatcgaaaaa ccgcagccgc tggcacgtcc ggtgatcctg tccaattcct
    12541 tcgcctttgg cggcaataac gccagcattc tgctcgggag ggtttcgtga gccactattt
    12601 atcccccggc gcttatctgc cccacgacgc gcccatgtta ctgctggaag acgtggtgag
    12661 cgtcagcgat gacagcgcgg tgtgccgcgt gacggtttcg cccagtggcg tactggcacc
    12721 gtttctcgac ccggacggca atctccctgg ctggtttgcc cttgaactga tggcgcaaac
    12781 cgtcggcgtc tggtctggct ggcatcgcca ccagcagggc aaaaacagta ttgagttagg
    12841 gatgatactg ggcgcgcgtg aattgctttg caccactggc attctgcccg caggccagac
    12901 gctgaccatt accgtcaaac tgctgatgca ggacgatcgc ttcggcagct ttgagtgcag
    12961 catcaacgac ggcgaggcga cgggccgcat taacaccttc cagccgaccg cggaagaact
    13021 taccaccctg tttcaacaag gagcgtcctg atgagtcgtt cagttctggt tactggtgcc
    13081 agcaaaggca ttggtcgcgc catcgcctgc aagcttgcgg cagacggctt taacattggc
    13141 gttcactatc accgtgacgc cacaggcgct caggaaacgc tgaatgccat tgtcgccaac
    13201 gggggtaacg ggcgtttgct cagtttcgac gtcgcaaacc gcgaacagtg tcgggaagtg
    13261 ctggagcatg agatcgcgca acacggtgcc tggtacggcg tggtcagtaa cgccgggatc
    13321 gcccgcgatg ccgcttttcc ggctttaagc gatgacgact gggatgcggt gatccacacc
    13381 aacctcgaca gtttctacaa cgtcattcag ccgtgcatta tgccaatgat cggcgcgcgc
    13441 cagggtgggc gcatcatcac gctgtcctcg gtttccggcg taatgggcaa tcgcggccag
    13501 gtgaattaca gcgcagccaa agccggaatt atcggcgcaa caaaagcact ggctatcgaa
    13561 ctcgcaaagc gcaaaatcac cgtcaattgc atcgcgccag ggcttattga taccgggatg
    13621 atcgaaatgg aagaaagcgc cctgaaagag gcaatgtcga tgatccccat gaaacgtatg
    13681 ggccaggcgg aagaagttgc cgggcttgcc agttatttaa tgtctgatat tgcgggttac
    13741 gtcacccgcc aggttatttc catcaacgga gggatgttat gacacgtcgc gtagtgatta
    13801 caggaatggg cggcgtgact gcctttggtg aaaactggca ggacgtttct gcacgcttac
    13861 tggcgtatga aaatgcggtg cgcaaaatgc cggagtggca ggtctacgat gggctgcaca
    13921 ccctgcttgg cgcgcctgtt gatgatttca cgctaccgga gcactacacc cgcaagcgta
    13981 tccgtgcgat gggccgcgtg tcgcaaatgt cgacccgtgc cagcgagctg gcgttagagc
    14041 aggcagggtt gattggcgat ccgatattaa ccagcggcga aacgggcatt gcgtacggtt
    14101 catcgaccgg cagtaccggt ccggtgagcg aatttgccac catgctgact gaaaagcaca
    14161 cgaacaacat caccggcacc acctatgtgc agatgatgcc gcacaccacc gctgttaata
    14221 ctggcctctt tttcggcctg cgcgggcggg tgatcccaac ctccagcgcc tgtacctccg
    14281 gcagccaggc gatcggttat gcgtgggaag ccattcgcca cggttatcaa acggtgatgg
    14341 ttgccggtgg tgccgaagag ttatgccctt cagaagcagc ggtgtttgat accctttttg
    14401 ccaccagcca gcacaatgat gcgccgaaaa ccacaccttc accgttcgat gaaaaccgcg
    14461 acggactggt gattggtgaa ggcgcaggta cgctcattct cgaagaactg gagcatgcca
    14521 aagcgcgcgg cgcgaccatt tacggtgaaa tcgtcggctt tgcgactaac tgcgacgcgg
    14581 cacatattac ccagccgcag cgcgaaacca tgcagtattg tatggagcaa tcgctgaaaa
    14641 tcgcagggtt aagcgcacaa gatatcggct atatttcagc gcatggtacg gcgaccgatc
    14701 gcggtgatat ggcagaaagt ctggcgacag caaccattta cggtgataat gtgccgctct
    14761 cctcgctgaa aagttatttt ggtcatacgc ttggtgcctg tggcgcgctc gaagcctgga
    14821 tgagtctgca aatgatgcgc gaaggttggt ttgcgcctac gctaaacctg aataaaccag
    14881 acccgaattg tggcgcgctg gattacatca tgcatgaagc ccgcaaggtt gattgtgagt
    14941 ttttgcagag taacaacttt gcctttggcg gcattaatac atccatcatt attaagcgct
    15001 ggccctgagt tatacaacca catggacatc ccatgtggtg attcttcaaa aaatctgaat
    15061 caatgaatat gaacctcaaa ttctcttacg catactgaag agcattccgt ataacgattc
    15121 agcagacaaa actttatcag tccacacgaa tcaacggatg cacagatgac acgcccggtt
    15181 atcaccgggc aatggttgcc ataacgggta acataaggga ctgaacagtt gtctttagcg
    15241 caacgaactg atctaacgta cctgaacgga caacctgaaa acagccttcc atcctgcatt
    15301 gtccgccagt gcttattacc aaccacgtct gttgcagtaa agtaatcagt tgcgaacatt
    15361 atttatcaat cgtgacgctg gctcgcgcat ggcagaaaag gtcctgactg agcaacaaca
    15421 ggcatcgtac tgttgctgct ccatgttgtt tatatgaacg catcccgttt gcaaggcttc
    15481 cgtgtttttg acgttgacgg tagattgctg ctctatatcc ttattgcagc cggtgctgcg
    15541 ggcctcaatg agattcgctg actctcgcct tattcacgca acgagcttta agctcagaaa
    15601 aacatccaag tttggtgagt cacagtctta cctgtcgttg tcatcaatat aggaatggat
    15661 aatctggaga gcgatactgc aggctgatat tgcggttttg cgacaagttt ttagcatccg
    15721 cttctacgcc aaatacctga agtaggaatg gatgcgttag cagcatacat ccggaactgc
    15781 gcggggaata gccggaaacg gctatcccta ccacaatagc taactttgta atagcgctct
    15841 tttaggctca tgtactctga cgtaattcta tctggtagtt gaattgcacg atacgcgact
    15901 cttctgattc atcatgatta ttattcaata gtccaatcag taacttacct gtctctatcc
    15961 ccattttttt gtagtcaata cggatggatg tcaaagttgg gtgtgtttga tcgcaattgt
    16021 ctgaaccgtc aagacaagct acagcgatat ttccagggat ttttaacaaa cgccgctggc
    16081 actcaaacat tatacccagc gcaatctctt cgtggctgca aatcacagca tccagctctg
    16141 gttgtcgcag taatatctcg tttaatgcgt aacgccccaa ttgcagactt gcggcatccg
    16201 gcatggtgat aacaagatca gtgttctggt aatggctcag cattgcttta tgccagccgt
    16261 tcagttgctg acgttgcagg cggttgtcca tatgcgcacc gataaatccg atattcttat
    16321 aaccctgctc cagcagatgt tcggtcagtt tatgtgatgc ttcaaaaaat gctgtttcga
    16381 tggtaatttg agccggactg aaaggggaac cgataacgtt aatggtaggt atatttgttt
    16441 tctcgagtat ctggtgagtt ttatcagcca actgagaacc aaaaataact aacgccgccg
    16501 ggttgccttg aagaagcgtc atcagaattt cagcttcttt gagctgatta tactcatgac
    16561 acccaagcag tagctggaat tcatttttat taagcacctg ctgtaatgac tgcataaagc
    16621 gggagctggc tttatctgtc agggaaggaa ccagtacggc gactgtatga ctgtgaccag
    16681 atgcaagggc gccagcagtt ctgttgggaa tataacccaa ctcttctacg gcctgttcta
    16741 tcttttccct cagcttgtca gaaacctgtt ccggtgttct cagcgcgcgg gaaacggtca
    16801 tggttccgac acctgcaaag ttagcgacct cctgcagcgt cacccgtccg gtaccacggc
    16861 gcttacgtgt tttttccatt tcgactccat ggataattac aaccagctgt tttcattact
    16921 tttatttcac cattactctt tccagttttt gcccacggta gcaaatatcc tgttgcattg
    16981 tatatgcagg aaccgaatat agccaaagca tggcggcaga gaaatgatag cgctatcaca
    17041 aagattggta gcgttatcag atagcgctat caaatgcgac aatcatcaca ttaattaatg
    17101 cgtaaaatat ctactctcct gttaattatc atcgggagtt atcagtagca tgttaaaaaa
    17161 tctattaaat acagaggtcg ttcaggtcgt ggaacaggtc aaagactggc gggaagccgt
    17221 ggcgatttca tgccgaccgt tgattgaaaa cggctctatc gaaccgcgtt atgttgatgc
    17281 gatctatcac tcgcacgaca ctatcggtcc gtactatgtg gttggccctg gtattgcgat
    17341 gcctcatgcg cgcccggaag agggggctaa caagctgtca ttagcactga ccctgatccc
    17401 ttcaggtgtg aattttgatg ccgatgaaaa cgatccagtg aaattactga ttgtgctggc
    17461 tgcaaccgac agtaccagcc acattgaagc gatttctcaa ctcgccaagt tattcgataa
    17521 cgaaaaagac atacaggcca ttttgacggc caaaacgacg caggacattt tgtctgttat
    17581 tgcgcgctat taatccagag gaacatttat gaaaattaca gtggtttgcg gtaacggctt
    17641 aggcaccagc ctgatgatgg aaatgagcat taaaagcatt ttgaaggatt tgtccgtcag
    17701 cgctgatgtt gatcacgtcg acctgggctc cgcgaaaggg acgccgagtg atatctatgt
    17761 cggcaccaaa gacattgccg agcagttagc cgcgcagtct gtcgcaggca aaatcgtcgc
    17821 actggaaaat atgattgata agaaagccat gagagaacgt ttgtccgtgg tattaaccga
    17881 attaggcgca ctgtaagctg ggggcgatat ggatttcttt cgttttttga tgagcgatgt
    17941 actttccgaa cccgcagtgc tggtcggttt aatcgccctg attggcctga ttgcacagaa
    18001 aaaaccggta accgaatgca ttaaaggtac cgtcaagacc attatggggt ttgtgatttt
    18061 aggcgcaggg gcgggtttag ttgtgtcgtc actgggtgac ttcgcaaaca ttttccagca
    18121 tgcctttggt attcagggtg tagtgccgaa caatgaagcc attgtttccg tagcgcagaa
    18181 aagcttcggg aaagaaatgg cgatgattat gttctttgcg atggttatca acattatgat
    18241 tgcccgtttc acaccgtgga aatttatctt cctgaccggt catcacacgt tgtttatgtc
    18301 gatgatggta gcggtaattc tggcaacagc aggcatgacc ggcattacgc ttatagccgt
    18361 tggctctttg gttgttggcg tggcaatggt ctttttcccg gctattgcgc atccgtacat
    18421 gaagaaagtg accggttctg atgacgttgc catcggccac ttctcaacat tatcttatgt
    18481 attggccggt ttcatcggca gcaagtttgg taataaagag cactcgactg aagacatgaa
    18541 tgtgccgaaa agtctgctgt tcttgcgcga cacgccggtt gctatctctt ttaccatgag
    18601 cataattttc ctggtgacgt gcctgtttgc gagtgcagat gcggtgaaag agcttagcgg
    18661 tggtaaaaac tggttcatgt tctctatcat gcaatccatt accttcgcag ctggcgtgta
    18721 catcattctg cagggtgtgc gcatggtgat tgcggagatt gtcccggcat tcaaaggtat
    18781 ttcagacaag ctggttccga atgccagacc tgctctcgat tgcccggtcg tcttccctta
    18841 cgcacctaac gctgtactgg ttggtttcct gagtagcttt gctgcaggtt taatcggtat
    18901 gttcacgttg tatctgctga acatgatcgt gattattcct ggtgtggtgc cgcacttctt
    18961 cgtaggtgca gctgcagggg tattcggtaa tgcaacgggt ggacgtcgtg gtgcgatttt
    19021 gggtgccttc gctcagggct tgcttattac cttcctgcca gtattcttat tgccggtgct
    19081 gggtgatatt ggttttgcta acaccacctt cagtgatgct gactttggcg cactgggtat
    19141 tttgttaggg attatcgttc gctaatattt tgatgacatt aatatcctcc aggatttcag
    19201 gaacgtggag gatattaatt cctcttattt tctccatttt tctttcttcc agtcaggact
    19261 gcatccgatt aatcgtaagc gctccgagaa gtacgtagcg taaggattat tttacagctg
    19321 agaagtttca gtgcagcagt tcatgcacgc ggttcgacgg ctagtcattg atcttcataa
    19381 ttacctcgcg taaaaaacgg ggtaaggccg ctcatccagc gtcatctcca caggtaataa
    19441 ttcaacgcca atgccagaag agttcgttac cggaagacgc cgtgatatac gccctttgat
    19501 cagcgagagc ctgagccatt tgaacagaag attatcattg atatcatgct cccgggcaat
    19561 acgggcaaca gaaccccttg gttgtaaagc cagtttaacc atttgaagtt taaactcatt
    19621 tgaaaataac ctgcggggtt cagctgataa tgttttttgt tccataacag gtgtccacta
    19681 gttgaaaagg tgggtaccta tgttaccaat actggcttaa tggctacata cagcagtcgg
    19741 tttacgcata cacttttata gtgcactccg gcctcatgaa tataacggtg gattaccgcc
    19801 gaacgaatcg gaaaatagat actggaaaaa aactgaaatt gtgaccagtt tttgttgacc
    19861 actacaaata aataaaagag ttgcagtgct tgctcggtaa gaaaacaatg gaggttaagc
    19921 tcctcaaaga agctgtggag tacaccactc tacagtagtt gcgcaaattt ggtgtggacg
    19981 ataatgtgat cgtctataag ggcaacgcta tcatagtctt gtcctggcgg gtaaaaaacg
    20041 cgcttacctt aacgataagc gcgccgctgt tcaggccttg agtggttatt caattcctgt
    20101 ggtgactgta aaagtgcgcg tttgctgcgg tgcaacctga atcagcgtgc cattacgttg
    20161 cgcggcaaga tacccctcag gccgacaggt tgcaggtaat gcaaaggcgg ctacctgttg
    20221 ctctccgtta taaaggatcc agcgtgtcac ataatttagt tcagcactgt agaaacgagt
    20281 aacaaacgta gtgccatcgg gagagatcat gcgaaactct ggctgatctg tataagcgtc
    20341 cagtttgtct gcaaagaaga caatttctgg atcataaaat tccggttgac tcagcgtcga
    20401 cagagaggct tctccctgca taatccgttg attaaacgcc agccactgag cggtgggatt
    20461 aacatgcgaa ggcactgatt cacgcaatct taatatttcg tccgggatat tctggctgaa
    20521 tgtagcattt ggtatatatg cataattcat gtggcacata tattgtagtg gcatatctac
    20581 agaagccaga ttggttacgg ccatcttaat atcgaacagt gtagaggatt tgtgaaggac
    20641 cactgttggc tgagccagat aatgatgacc gaaacccatt acatactcgt aacgcccgtt
    20701 aaggcgtaac atatctccgt ctaattccag ccatgcttca tccatcgcgg cacaggccat
    20761 ttcaccgtgt agcagatgag tatcttccgc agatgggcag ccattagcca gcaaacctga
    20821 atgaaaagca aaacagccat aggtctctat cacctctgtc gccggtttag gctggcgaaa
    20881 catattgcac atggtgaggc cgtgtccatc aaattgcgca tcccaaatca tctgccccat
    20941 ccagggaaga ataatcaaat gtccacgact gtttgcaatt ttaagcccct cgacaccgct
    21001 gtcatatcga aaagacgtga cagtaaaatc actattttcc agcaagatac gaggtttctc
    21061 gccaaaaagc gcccgccaca aattaatacg cgtactcata acggttctcc tcaggacgct
    21121 gtgacttcag ccagtgcggt acgtactttg ctttcacgcc agaagtagac tccgacatag
    21181 acaaagcaga gcatagaaac caggaatgaa agctgtagtg agtggaacat atctgcaata
    21241 tatccctgaa ttgccggaac caccgcggca ccgacaatag ccataacaat gactgctcct
    21301 gccatttctg tatgttcgtt atcaactgta tccagtgttc ctgcatagat cgtcgcccag
    21361 caagggccaa acaaaacact taccaggacg gcgacataga ccgcgctgaa acttggagcc
    21421 agtgcaacat atgccaagaa cagcgcccct ataacggaat agagaatcaa tactttttcc
    21481 ggattaaaac gcgtcataag gatattggct ataaacttgc caataaagaa gcaggcaaag
    21541 ctatagacca tgaagtttga agcatcacgt tcgttgatat cgcccaactc cagcgccaga
    21601 cggatggtaa atgaccatac tgcgacctgc atacccacat aaaggaactg cgccacaata
    21661 ccgcgacgaa agcgcggatt tctagccaga tagcgcagcg tatccattgc tgacgggcgt
    21721 ttatggtgac ttgtctgtgc cactttacag gttgggaagc gggttaaaag gaacaacacc
    21781 atgaccacaa ccagaatcat aatcatatac ttatacggtt caagggtgtt ctctaacatc
    21841 agcaccttaa agttgtgaat ttgctcggcg ttcattcctg acatctgctt ttcaaggctt
    21901 tccccctcgg agaaaaccag atatttgccc aataaaatac cagacgcagc accaatcgga
    21961 taaaaggtct ggctgatatt gagccgcaat gtggcatagg cttttggacc gatcattgaa
    22021 ctgtatgtgt tcgctgcagt ttcaaggaaa ctcaggccaa tcgcaatcgc aaaaatagct
    22081 gcaaggaaca tagtgtaggt tgccatatgc gaggcaggga aaaaaagtgt acaaccacca
    22141 atatacagcg tcaggccaat taaaattgcc accttataac tggtcttttt aatcacaagg
    22201 gatgctggta ttgcaattaa aaaataacct ccataaaatg cgctctgcac caatgctgaa
    22261 gcaaagttac ttagcgaaaa tacacttttg aattgagtga ttaatatgtc atttaatgca
    22321 gctgcgcatc cccatagcgg gaataaacac gataacaaaa taaactggaa caagggagtc
    22381 ttattcagat acccatcagg catctgaatg atgtttttat cgttcatagt gctaccttta
    22441 actgtgcagg atgattattc gtttaaggtt aaaaattcat taaattgttc aatactcgga
    22501 taagatgatt gcgtaccttt ccctgtgacg ctgaaagcgg caaagagagc ggcttttttc
    22561 aaagcggctt caacatcacc gctttgaaca taataatggg aaaagcaacc aataaatgcg
    22621 tcaccagcgc cactagtatc aacagcattt actttgaatg caggaacatg gacttcctga
    22681 tcgcgggtca tccataatgc gcctttttcg ctcatggtaa caataatatt gttcagccct
    22741 ttatcaacta acgaacgtgc ggccaaacga atatgatcat aagtatcaac cgacataccg
    22801 gttaatattt ccagttctgt ttcattcggg ataaagaaat cacatttgca ggcataagac
    22861 atatctaact cacgcaatgc cggagccgga tttaataaca cttcaatacc atttttctta
    22921 ccaaactcaa tcgcgtggta aactgtttcc agttgaactt ccagttgtaa aacgatcaat
    22981 ttgcattttt tcagatcttc tgcagctcga tcgatatctt ccggggaaag aaatttattc
    23041 gctcccttaa ttattaatat actattgctc gagttggcat taacaaagat cggtgcaaca
    23101 ccactgctgg tacaggggac tttctcaaca taagtggtat taattcccca tgattcgaga
    23161 ttacgaatag tattatccgc aaaaatatca tcacctactt tagtcagcat caggactttt
    23221 gaattcaatt tagccgccgc caccgcttga ttagcacctt tcccaccaca tccgattttg
    23281 aaggcaggtg cttccagagt ttctccttct ttaggcatct gattagtgta agtaatgaga
    23341 tccaccatat tggaaccaat aactgcaatg tccatttcac tacctcttat aaactttcgc
    23401 ataacaatgg tatttaaata acattagcat gttacttttg catcatttgt gactgagatc
    23461 gcgattagca catcaacccg atgtttattt aatagacttc cagtcttatc actcaggcta
    23521 acactatcta atcataagca acctaacagg attaataccg aaaattcagc agtctatacc
    23581 cttttcattt caaagggtcg gtcgtatagt atggtaacta aaacaatgtt tactaatgcc
    23641 ataatgttat ttttataaca ttttacggag agagttgatg gaaacgaagc aaaaagagcg
    23701 tatccgacgt ttgattgaaa tacttaagaa aaccgacaga atccatttga aagacgcggc
    23761 acgaatgctg gaagtttctg taatgactat tcgtcgcgat ctccatcagg aagatgaacc
    23821 tctgccactg accctactgg gtggctatat tgtaatggtg cataaacccg caccatccat
    23881 gccagtaatc caggacgttc cgagaaatca tcgtgatgac ttacctattg caattctggc
    23941 cgccggaatg gttaatgaaa atgatctgat cttctttgat aatggccagg agataccgct
    24001 cgttataagc atgatcccgg atgcaatcac cttcactggc atctgttact cacatcgtgt
    24061 ctttgttgcg ttgaatgaaa aacctaatgt gacagcaata ctttgtggtg gtacgtatcg
    24121 tgccagaagt gatgcttttt acgatgccag taactcttcg ccattagact ctctcaatcc
    24181 gcgaaaaata tttatttccg ccagcggtgt acatgatcac tttggcgtca gctggtttaa
    24241 tcccgaagat cttgccacta agcgtaaagc gatggcccgt ggactaagga aaattttgct
    24301 cgcccgccac gccttgttcg atgaagtagc ctctgccagc ctcgcaccgc tctctgcatt
    24361 tgatgttctg attagcgagc gtccgttacc ggcagattat gttacgcact gccggaatgc
    24421 ttctgtaaag atcattacac ctgattcaga agacgaatga cttactgaaa aaataccgta
    24481 ctcttgttaa acatcgtcgg attggactga ttacgttgca ctttcatcac atattccagt
    24541 ttatcaattt ggcttatcat ctactccaga cgctggtcat cattgaccaa tagtctgata
    24601 tggcttttgt cgctgtcctg aatcggcagg cagaaaatgc cttcaacgtt aaaagcacga
    24661 cgggaaaaag cccacaaagg ttagtcatta cgcccggata ataaacaggc tcctccctgt
    24721 tatggaagcc gttgtattgt ccataatgtc tgggttttta atgcacactg gataacaacc
    24781 ggacgaatct tcaaatatta ctggcgctga cagccggttt gaattcatct cacaaccctg
    24841 catagagcga atctcctgcc tgcacgtcac tccactccat ggtatcaact tcacactctt
    24901 tatctgcggc tagtttcagt caccagataa gcatcgacta tgaaaggtga gccatgacaa
    24961 cataacgttg gtaacgctct gacgccttaa cggaagatgc ctgccaccac agggaatgta
    25021 aacgactgaa gtgtggcctt taatgccgtg aacggctcat ggtctcctgg cacggttgcc
    25081 gccccaacct gtaacaacat tccacagtac aatgtctgtc agagccggag cctcccatgc
    25141 ctgttgaata aaaccccgga tgcttccccc gattcttcaa gtaatacttc ggttatttac
    25201 actattttat ccctgttgaa tttggttaag ccggtgcaga cgcgccggga aacgcacgcc
    25261 tgaaccggca cattaaaata caatacaacc ccctgttact ggagatacga atggaatgac
    25321 gaggcagcct gtcagtcaca ggagggctgt tggctcaaaa tcaacggaaa ggccaccaac
    25381 accggaatta agctccacag tccccgcact ggcaatccat tcccgggaac ggcattaccg
    25441 caaactgctc cggaggcaac cattacagcc tctgtaacgg attatcagcc ctggcatctt
    25501 ccgacggcac ctcctgccgc ccccacgc
//
LOCUS       DADTFG010000064        25164 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.064, whole genome shotgun sequence.
ACCESSION   DADTFG010000064 DADTFG010000000
VERSION     DADTFG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.064"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..157)
                     /gene="safA"
                     /locus_tag="J1138_002416"
     CDS             complement(<1..157)
                     /gene="safA"
                     /locus_tag="J1138_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBA8454204.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIA"
     gene            complement(405..1544)
                     /locus_tag="J1138_002417"
     CDS             complement(405..1544)
                     /locus_tag="J1138_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding domain
                     protein"
                     /protein_id="HBA8454205.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LDLAVHVATKLNRSHLLTARHSYILPVLGRSEIDMQKSGAQAVTVEDSMSMIHASRGV
                     LKPAGVMLKSECAVVAGIAQAALPQSVVAWEYLVEDYDRIRNDIEAVLPEFADYNQRI
                     RHPGGFHLINAAAERRWMTSSGKANFITSKGLLEDPSSAFNSKLVMATVRSHDQYNTT
                     IYGMDDRYRGVFGQRDVVFMSAKQAKICRVKNGERVNLIALTPDGKRSSRRMDRLKVV
                     IYPMADRSLVTYFPESNHMLTLDNHDPLSGIPGYKSIPVELEPSN"
     gene            complement(1883..2797)
                     /locus_tag="J1138_002418"
     CDS             complement(1883..2797)
                     /locus_tag="J1138_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8454206.1"
                     /translation="MGKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANAAPDATKASGVGVSLIRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
     gene            complement(2857..3360)
                     /locus_tag="J1138_002419"
     CDS             complement(2857..3360)
                     /locus_tag="J1138_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA8454207.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDVITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(3373..3903)
                     /locus_tag="J1138_002420"
     CDS             complement(3373..3903)
                     /locus_tag="J1138_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA8454208.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            3892..4313
                     /locus_tag="J1138_002421"
                     /pseudo
     CDS             3892..4313
                     /locus_tag="J1138_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015584502.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(4520..4744)
                     /gene="hipB"
                     /locus_tag="J1138_002422"
     CDS             complement(4520..4744)
                     /gene="hipB"
                     /locus_tag="J1138_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBA8454209.1"
                     /translation="MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATTSL
                     PTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            complement(4802..5617)
                     /locus_tag="J1138_002423"
                     /pseudo
     CDS             complement(4802..5617)
                     /locus_tag="J1138_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5814..7265)
                     /locus_tag="J1138_002424"
     CDS             complement(5814..7265)
                     /locus_tag="J1138_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBA8454210.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVLGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(7492..8910)
                     /locus_tag="J1138_002425"
     CDS             complement(7492..8910)
                     /locus_tag="J1138_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA8454211.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(9049..9408)
                     /locus_tag="J1138_002426"
     CDS             complement(9049..9408)
                     /locus_tag="J1138_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBA8454212.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(9408..10334)
                     /gene="glsB"
                     /locus_tag="J1138_002427"
     CDS             complement(9408..10334)
                     /gene="glsB"
                     /locus_tag="J1138_002427"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBA8454213.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(10398..11786)
                     /gene="sad"
                     /locus_tag="J1138_002428"
     CDS             complement(10398..11786)
                     /gene="sad"
                     /locus_tag="J1138_002428"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBA8454214.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKIAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRN"
     gene            11887..12768
                     /locus_tag="J1138_002429"
     CDS             11887..12768
                     /locus_tag="J1138_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8454215.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            12846..12929
                     /gene="yneK"
                     /locus_tag="J1138_002430"
                     /pseudo
     CDS             12846..12929
                     /gene="yneK"
                     /locus_tag="J1138_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            13107..14297
                     /gene="ydeA"
                     /locus_tag="J1138_002431"
     CDS             13107..14297
                     /gene="ydeA"
                     /locus_tag="J1138_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBA8454216.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(14322..14987)
                     /gene="marC"
                     /locus_tag="J1138_002432"
     CDS             complement(14322..14987)
                     /gene="marC"
                     /locus_tag="J1138_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBA8454217.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            15199..15633
                     /gene="marR"
                     /locus_tag="J1138_002433"
     CDS             15199..15633
                     /gene="marR"
                     /locus_tag="J1138_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBA8454218.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            15654..16037
                     /gene="marA"
                     /locus_tag="J1138_002434"
     CDS             15654..16037
                     /gene="marA"
                     /locus_tag="J1138_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBA8454219.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            16069..16287
                     /gene="marB"
                     /locus_tag="J1138_002435"
     CDS             16069..16287
                     /gene="marB"
                     /locus_tag="J1138_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBA8454220.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(16344..17783)
                     /locus_tag="J1138_002436"
     CDS             complement(16344..17783)
                     /locus_tag="J1138_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA8454221.1"
                     /translation="MSGFKKDFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAGMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIHFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDLNEREQIMYQAVHYEL
                     VASALAVQTGKLINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPDDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFSSHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(17808..18902)
                     /locus_tag="J1138_002437"
                     /pseudo
     CDS             complement(17808..18902)
                     /locus_tag="J1138_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
     gene            complement(18893..20029)
                     /locus_tag="J1138_002438"
                     /pseudo
     CDS             complement(18893..20029)
                     /locus_tag="J1138_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(20144..20455)
                     /locus_tag="J1138_002439"
     CDS             complement(20144..20455)
                     /locus_tag="J1138_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA8454222.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHATAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            20654..21352
                     /locus_tag="J1138_002440"
     CDS             20654..21352
                     /locus_tag="J1138_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA8454223.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(21397..22296)
                     /gene="eamA"
                     /locus_tag="J1138_002441"
     CDS             complement(21397..22296)
                     /gene="eamA"
                     /locus_tag="J1138_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBA8454224.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            22491..23678
                     /gene="ydeE"
                     /locus_tag="J1138_002442"
     CDS             22491..23678
                     /gene="ydeE"
                     /locus_tag="J1138_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBA8454225.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWSMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            23805..23900
                     /gene="mgtS"
                     /locus_tag="J1138_002443"
     CDS             23805..23900
                     /gene="mgtS"
                     /locus_tag="J1138_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBA8454226.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(24119..25009)
                     /gene="dgcZ"
                     /locus_tag="J1138_002444"
     CDS             complement(24119..25009)
                     /gene="dgcZ"
                     /locus_tag="J1138_002444"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBA8454227.1"
                     /translation="MIKKTTEIDAILLNLNEAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSSMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         6821 a         6123 c         5557 g         6663 t
ORIGIN      
        1 ttgcaatgcg acccatttct gaaataccca gctgatcaat tgccgaaaaa atggctatca
       61 atgtaagggt cagtaataag acaaccacaa ttacagacat gatttcttta tagttgtctc
      121 tttgcgtgat tttgtttttc actgtggtcg catgcatttc aaatgtgttt atttagcgga
      181 taatgttaaa aatcgttaat cagtatgatt aatataacag gctggataaa agaggaaata
      241 ggactggcac ttcagtactg agcggagttt cttacagctg taggcagaag tttatatctt
      301 atagtgtgtg gtttaattta atccatgtga gcaaattgcg cgataatagt caatgaggaa
      361 ttcttctgct gtgcggatga cagcagaaga aatgaggagg cgaattaatt tgatggttct
      421 aattcaaccg gaatactttt atagccagga atgccactta atggatcgtg gttatcaagt
      481 gttagcatgt gattcgattc tggaaaatag gtcaccagtg agcggtcagc catagggtaa
      541 atgaccactt ttaatctatc catgcggcgt gagctacgtt taccgtctgg cgtgagtgca
      601 ataagattaa ctctttcgcc gttttttaca cggcaaattt tagcttgttt agcactcata
      661 aagaccacat ctcgttgacc gaatacccct cgatagcgat catccatacc ataaatcgtc
      721 gtgttgtact gatcgtgact gcgtactgtc gccatgacca gcttactgtt aaacgctgaa
      781 gagggatctt ctaacagccc tttactggta atgaaattag ccttacctga cgacgtcatc
      841 cagcgccttt cagctgctgc gtttatcagg tgaaaaccac caggatgacg gatgcgctgg
      901 ttatagtcgg caaactctgg cagcacagct tcaatgtcat tgcgaatgcg atcataatct
      961 tccaccagat actcccaggc taccacgctc tggggtagtg ctgcttgcgc gattcccgcg
     1021 accactgcac actctgattt cagcattaca ccggcgggtt ttaacacacc acgcgaggca
     1081 tgaatcatcg acattgaatc ttcaacggtt accgcctgcg caccgctttt ttgcatgtca
     1141 atctcgctac gtcccagaac cggcagaata tagctatgcc gtgcggtcag cagatgagag
     1201 cggttaagct tagtggctac gtgtaccgcc agatctaatt cgtgatctcc ccaggtaagt
     1261 aatgattgaa ccgtattctc agcaaagaaa gaggcattta cctgcttatc cgtgacttcc
     1321 caggcgattg cttttgcgcc gttttcacaa atgtcgaatg acgcactgtg cttaggatct
     1381 ggccatgcac atcccggaca gtcaaagccc tctggcttat tcatgtcaaa catggcaata
     1441 acatcctggc gtatatccat ctgcttacgt actgcattcg ctacggattt aacagcaccc
     1501 caaccaccgg cagctccctg gtaggattca atttttttct tcatctgctt atcctgtgtg
     1561 gaaatgaatt attaggattt gtaatagcga tgatgcaaag tatatacaaa ggatttatcg
     1621 tcaacatgaa tatataagag taggggacta ctctcttact atttttactc tattatcgtt
     1681 aaataataga gtagtataaa tacgataagt taagcatcat ctctcatagg gtttaaatta
     1741 acatgattct caatataaaa aataagactc tggcttcaat tgtgcgcgga ttttcttaca
     1801 agtgtaggat aatagccttc acaaaataat tatccgcgca attttcgtga atagagcgca
     1861 tagactaatg tcaaaatcac aattattcat agataaaagt gacaccaatg actgactgga
     1921 cggctccggc tgcaaccttg tttcctgttt gtccataggt tgcgcttaaa cccagaggta
     1981 ctttgctttt atttactgtc cccagggaga cattttctcc tgttgccagt atttttccat
     2041 tacgtatcag agagacaccc acgcccgatg cttttgttgc atctggtgct gcgttagcaa
     2101 acacttgtcg ggcactgtca gtggtggcac ccgaaagata aaatgagagc ttttgttcgc
     2161 tagagcaata tacaccaagg ggtatttctg cgcttccagg aaagtccggg agattcacgg
     2221 tgacattacg actatcaacg gtgcaaccgc ctgttggcat aacaacactg ttatttgaaa
     2281 taatattcca cgtaaaatta cgcggatttc cgcttcctaa cgtggcaatt ttatacatat
     2341 gaatacgggc aataacttcc cctgctttta tgacaactcc accggccgct ccgactgggg
     2401 taatatagag ttttaacggt aatggcatcg gtgtcttatc accaatatcc aatacgttgg
     2461 tatttgtcgt caacgggaaa gggtaggtca cattattcca gtacagtgat cctttatatg
     2521 actgtaggga gcctgcgaaa gctgatcctt gtaccaggtt tatatgatcg gtgtcgtacc
     2581 agccgccgta atcattccaa caacttatat gctgagacaa gtctacaacc agattctggc
     2641 ccggttgtat tacaggatcg agattaacat aaacagaggt tgtacctgct ccaatacttg
     2701 acccgccatc gacattacaa ctgaaggcaa aaactttgcc tgccattaat agaagataaa
     2761 taccgaacag gacctttata ctgaatgttt tacccatgat atatcctaag gttaaaaaat
     2821 tgatttaaaa aggaggaaaa taaaatcaaa ttgatgttat tgccaggtat agatcacatt
     2881 gattagcgcc tcgatcgtac cctggcttgc gtttccattc accgtgatag ctcttgcctt
     2941 aagtggaaac tgtgcattac gagtgatcac atcaacaata accgttttgc tatcgccatt
     3001 ttttaacgtc gcatcctggt catcccttag ctctatctga atattttcgg cagtaccttc
     3061 atttttgtaa taacccgtat tgtcagttga acctgtcacg attgccgtca ctgcacttgt
     3121 ttcaatcgga caatcggtta atgacaaagt aatattgtgc cagccagatg cagaaccagg
     3181 ttgttgcaga ttgcgtgtat aaagatcccc gagattaacg ttagcttctt tggtttgaat
     3241 agtgcatggt ttagcgacta cccgaccatt aacagtgata gtgacatcgg cggcctggag
     3301 agttgctgtg aataatgtgc aaaacgtagc taacaggaac ctcttgtgaa gtcttttcat
     3361 atctgactct gattattgaa attcaagggt aaaggttgcc gaagcgctca ccagtcctgg
     3421 attgacggac ttctgagttg acttcaaacg agcggagtaa ggcaaaatat tgttctgttc
     3481 tggtaccaat gggatccact gcatcccagc atgaaggtca ttcaatttca ccggacgcat
     3541 atttccgtcc agtatttcta tacccaaacc ggaggccgta ttgcttcctt catctagttt
     3601 caacagagta ttattttctg catcctcaat accgttaaat gcaacccgaa ctcccgttgt
     3661 ccctttgctg cattcactta atgtaatctg aaaagggacg actggacttg tgctaccggt
     3721 cgttggaaat tgtctggcac tgtttttttg gagatctacg gtaaaattaa gcgaatccga
     3781 tgagactgtg cagccataat cgaggacgcg cccgctaatt ttaataacac tatctgcgga
     3841 taaagcagaa taggtggtta accccagaca taaaccgagg aaaataatgt tattgtattt
     3901 cataatctat tgttccttag cgacagattg ctgtctgctg gttcagtaag gtaccaggag
     3961 aaaccgttta ctctcgtcta tgacggggct atcaccgaaa atgtcgcagg caaggtcaat
     4021 atccatcctg tgaaatatga tctgcatggc attcagattg cagcgaatgt gtatacacct
     4081 gctaactatg accctgcgaa aaaatatccg gcaatagtgg tggctcatcc gaatggtgcg
     4141 ttaaagagca ggtcgccggt ttgtatgcgc agcgccttgc tgagcatggc tatattactg
     4201 ttaccgccga tgcagcttat cagggggcta gcggtggggt gccacgtaac gtggataaac
     4261 ctgcgaatcg tattgaagat atccacggta tggctgattt catcagccag tatcattttt
     4321 ctaccgaaac gggagggaag tcagaaatca caacgtattg atttatctgt gtgtggatgt
     4381 gaatggtgaa gtaaaggacc ttcacaggca atttaacata atatacatta tgcgcaccaa
     4441 tataagccaa gggagaatcc agtcgttggc ggttcatgat ttttgttctg ctcattaaca
     4501 acgaccaaaa ccccatatct tacttaatac cgtattctcg gcttaaccga ccgtgcaacc
     4561 tcaacacatt ggtttcaact gccgtcacca cgttctccgg aaaatctgtc ggtaatgaag
     4621 ttgtcgcctg cttaatgccg atttttttcg ccaactcgct ctgcgtccag ccgttttgct
     4681 ggcgaaccag tttcattgca ttcgccaatt gcattgggct atagatcttc tgaaagctca
     4741 tcatgtcata cgtccacagc aaatttatcc gcttaagggg atattatcag ttttatcctt
     4801 tatccagcgg taaatcattg ttttcagtac cgaagggatc ttccagttct tccgccagac
     4861 aatccagcga aataaaagtg taggaaatca gcacagagat aaaaggcgtc atgtaatgca
     4921 ggtccacgac cagcgcaaac ggcagcatga tacaaaacag atacacggtg cgatgcaaaa
     4981 tcagggtgta ggcaaaggga attggcgtat aggcaatgcg ctcgcatcct gccaggaccg
     5041 ctgaaatatc attaagccga tcgttgaggc taataaacag gatatctgaa agttgtccat
     5101 tgcgtcgctg aaccgccaac cactccccca ttattaacaa gatgcggtta gctggagagt
     5161 tagaagccag tacacgctga agatcttcag tcttgagata atgagccagc acttccgcct
     5221 gtggctgttt gcgtaatgtc atgcgtaaac agtgggcgaa ggcgatttgc agccgggcaa
     5281 actcccttac acttgccgaa tccggcaatg tcgtttttac ctcgcgcagt aacgaccgcg
     5341 aggcaatcat caactgcccc caaagttttc gcgcttcaac gtaacgggcg taaccggcat
     5401 tattacgaaa accaagaaaa atggcgatgg cgacaccgag aatgctgaac ggtgcgaggg
     5461 tgaatttaat gcccaaatgc gtgtaccagg gcagcatgaa aataacagcg atagaaaaga
     5521 gaaaattgag tagtaagcgc gaggatatct tggataatac tgaaccgtgc cagacaaaaa
     5581 tacggcgcag ccagtgttgt tgtggacgaa caatcatggt tatcttcagg cgtggaaaag
     5641 tcgccctatt aaacgtgatt acgatcacat tctcaagacg ctacttacaa attacctaca
     5701 agcttacaac attaacaggt gctgcatgtg gcacctgtta atgataaagg cacatagaat
     5761 gttgtaacta atatagcgaa ataagaaacc cggtcgaaac cgggttcaga gtattagcac
     5821 aacgggcgta cagcttcgcg catccctttt tcgagaatcg catccaggtc attagcaacc
     5881 tgctcgacca gaccaagcac ttgcgtcagg tcctgctccc agtggtcttt ttctgccaac
     5941 acaatcgcta ccagttcctg ggtcccgata acacggtcac gatactggct ccagagttgc
     6001 tgataacgtt ccagccagtg tgcatcgtcc tgtaccggat atgtttctcc attacgctca
     6061 ccgcgataga acgcaatcaa tgccgctaat gcgaaagtaa ggcgcgccgg aagtgtgccg
     6121 tttgccttct gccctgccag cagctgcggc aggatgcgtg tgcggaactt ggtcataccg
     6181 ttgagcgcga tagacagcag ttgatgcttg atgtacgggt tacggaaacg tccggtgact
     6241 gcactggcga aagactccag ctcatcgcgt ggcaaatcca gtaccggaat aatttcttcg
     6301 tagatggctt tttcaacgaa tgcgcaaatt tcagcatcgt tcatcgcttc acctacggta
     6361 tccagccctg cctggaacgc caccggcacc agcgcggtgt gcgcaccgtt gaggattgcc
     6421 actttgcgtt ctttatacgg tttaatatcg tcaacgatca gcacgttgag cggatatttg
     6481 tccagacgaa gttcagtcgc taaggatttc ggcccctgaa tcacaaacag gtaaaagtgt
     6541 tcagcggtgt cgaggaaacc atcgtggtag cccagttctt cttccagttt agccacttca
     6601 tcacgcggat aaccggtaac gatacggtcc accagcgtag aacagaagct gttagcctga
     6661 tccagccatt gaataaatgc ttctggcagt gcccactctt gcgcatagcg cagcaccagt
     6721 tcacgcaacg cgtcgccatt atagtcaatc aactcacacg ggatgatgat ccaaccttta
     6781 tccagcgcac cgttgaaatg gctaaaacgt tcgaacagca gccgggtcag ttttgccgga
     6841 taacttaccg ctggcgcgtc atcgaattta tcgcccgcat ggtagctgat acccgcttcg
     6901 gtggtgttag agaaaacaaa gcgcatttcc gggttgtgag ccagtttcag gaattcatcg
     6961 tattcactgt agacgctgat ttcacgatta accgagcgaa tcagacgcgc gtcgctgacc
     7021 gcttcaccct tctcattcag gccacggata atggtggtat acagaccatc ctgcgtgctc
     7081 agtgacggcg ggaatgaagt ttcaatcgga cgaacaacga ccacgccaga attcagatcg
     7141 gtgtgctcat tcaggagatc gatttgccag tcaacaaagg cgcgcaggaa gttaccttca
     7201 ccaaactgaa tgatacgttc tggatactgt gcaccgggaa aatcgcgacg atttagtgtt
     7261 ttcacaatgg gttcccttct gattagtcat acaacctgtt taaattggta cgacaggtta
     7321 gcaaacttta atacgccgaa cccctgtttt gatcaactcc tgatgattaa tgagcagttt
     7381 tatgagaaaa gtgtggcgcg gatcatggtt taatcgagga aacaggcctt ttcttggatc
     7441 ataaagtggt agaacacatt gcatttaatt cgctctgagt aaataaagat gtcagacaac
     7501 ttcctcaccg taacgcatag tgctggtacg gttgcgccca tctttcttcg agcgatacag
     7561 acacgtatcg gcttcgacca tcaatttatt aaagtcatcg gtcagcgttc ggtacgatgc
     7621 gcaaccacta ccgacgccaa tacttaccgt gagataaagc gttttttgtt gccaggtgaa
     7681 cggttgaagt tctacgcctt tacggatttt ttccgccatt agcaggccat cgacaggatt
     7741 caccgacggc actgcaacag caaattcttc gccgcccatt cgtgccacca gccccttatc
     7801 accgacaatc ttctgaatat gctgggcaaa cacgcttaac actttatcgc cacattcatg
     7861 tccatagtta tcgttaatgc ttttgaaata gtcgatatca agcagcatga ccgtcagatg
     7921 ttgtgtctgt ttcagcgatg gacttttcag cgcctcataa agaccggacc gtgagtaaac
     7981 ctgggtcaga aaatcaaagt cggctcgcag cgcaacttgc ttcattagcg aattgatcgc
     8041 tgccacgcta aaggaaacca taattgggca tatcgccatc gtggcaatac cgagacgtgc
     8101 ggagaacatt tgcggaatgg agaacggcga accgaccgag atatcaatca ccgaatttgc
     8161 caccagtacg acttccacag caccggtgac aaaggtcaac agacatgtta cctgcggcgt
     8221 atagcgcact gcacaccaga ttaatgcagg gagcggaaac gccagactcc ccgctccgcc
     8281 aatgactacc gaggctataa ccgacacaat aagcgcgata gcaggcatca tctgctctgc
     8341 tttaaagcgc ggcagcactc caggaattgc caacgtcagc atacaaggca cgatcaacac
     8401 gcccgttgag aattgctcac taaaccagtc ggcaagcaaa ggccagaaat ccagactatc
     8461 aatactgacc gaaccaatcg ccccgacaat agcgcataat aatgcggcaa gcagacagta
     8521 attaaatagc cgtaaggcac ttactggctc atacttattt tttcccagac gcttatcccg
     8581 tgcgaccagt aaggcgacgg taacaataaa catcatattg gagaaattga tagccagtga
     8641 aaccagcccc cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc
     8701 atagtaatgc agtcgattaa gccagacata gcgggcaaac acccccgcca ttacgccatt
     8761 cagcggccag aaaagagaaa gttcgtgaac cagtcgtaac tctgcaccaa taaaatagaa
     8821 cagcgtggtt agcacaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa
     8881 acggaaagtt gaaatgggct gtacatgcat taattatctg tccatacaat tgcgggtggc
     8941 aattatctca acgcaataag taaattctta gaaaggatag catgttcgct gacaacggcg
     9001 gcaactaaag atacctaatg aatgccattt tatcgatttc cgtaattatc atggctgatt
     9061 catctgtata accagccagt ggtagattac cgtcacaata taacgttgtt gctcctcact
     9121 taacgcttcc ccctgaggaa tgttatgcca cttagccaga caaccgcgac aacaggtcgc
     9181 ggtggcgtgt tgagcgataa aaaccggatg cccgcgcatg ggggtttgct taccgtcgtt
     9241 agccggtaat gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcaatgac
     9301 tggcgcgcct ttctccagac aatactgacg ctctttcaca cccaaacgaa agcgagagcg
     9361 aaattttgaa cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaactgaa
     9421 cgccctaatt gtttcgtcaa ttgttcaagg actgcaatgc ccgcaagcga gttaccggca
     9481 tcatccagtt ccggactcca gacagcgatg gccatttcat gcggaacaat cgccacaata
     9541 ccgccaccaa cgccagattt tgccggtagt cccacccgcc aggcaaactc ccccgcgttc
     9601 tggtacatac cgctggtcgc catcagcgcg ttaatttgcc gcgcctgcat tggcgtcacc
     9661 acaggttcat caatatgaat agctttcccc tgattcgcca gaaagacaaa cgtccgggcc
     9721 agctctacac agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc
     9781 acgtcatgat ggaaattgcc aaacgacttc atcagccatg cgatagccgc atttcgcgcg
     9841 gaatgttcaa attcggaacg cgctaccacc gtatcgtagg aaatatcaga cacaccactt
     9901 aagccgcgca cgacttccag catacgttgc cgaggagcgc ttaatcgccc ttgcaacata
     9961 tcgcagacca ccagcgcacc ggcattaata aacggattac gcggaatacc ctgctccatt
    10021 tccagttgca ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag
    10081 atttcctctt cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac
    10141 tgaatggaaa aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag
    10201 atagcaatcc ccaatcggga accgtctacc gtagccagcg ccggaatata atccgcgact
    10261 ttaccctgac caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc
    10321 atggcgactg ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca
    10381 gagctgaata tgtagcgtca gttccggtct ttccacaccg tctggatatt acagaattcg
    10441 tgtaagccaa aatgggaaag ctcacgccca aagccactct tcttcacgcc accaaaggcg
    10501 actcgcgcat cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc
    10561 gccatctgtc tggcctgtgt ttcgtcggtg gtgaaaatgg tcgctgaaag gccgaactca
    10621 ctatcattag ccagttccag cgcatgttct gcatctttcg caatggtgat tgccgcaacg
    10681 gggccaaaca tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt
    10741 ggcggatagt agttacctgc cccagcaatc ttttcccctc ccagtaacaa acgcgcaccc
    10801 tgcgccagtg ttttttccac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt
    10861 ggtccgagag cgttctcttc atcacgggga tcgcccattt tcaaggctgc cgcagctgcc
    10921 acaaaacgtt cggtaaatgc cgaagcaatt ccctcttcga tgataaagcg ttttgccgct
    10981 gcacaaacct gtccggtatt ctgataacgt ccggctaccg ccgctttcac cgccagttcc
    11041 agatcggcat cgttaagcac aataaacgga tccgaaccac ccagttccag tacgcatttt
    11101 ttcagtgccg ctccagcctg tgcgccaata gccgctccag cacgaacact tccggtcact
    11161 gtgacagcag caatgcgcga gtctttaatc atcagactga caccgtcgtt gtcggcattc
    11221 agccagccat atacgccttg tgggataccc gcatctttaa acacctgggc aatgagctgt
    11281 gcacagccca tcacattcgg cgcatgttta agtaagtagc cgttaccagc aagaatgatg
    11341 ggaacagcgc cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc
    11401 gtccccaacg gtcgatactc aataaccgcc tgctgatttt ccaccagcgt aggttctgcc
    11461 ttcagcattg ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact
    11521 tcagcgcgcg cctggttgat tggtttgccc atttcacggg tgatcatttg cgccatttct
    11581 tcgctacggg cgcgcagagc cttaccgata tcacgcagtt tttcagcacg ataatctata
    11641 tttgtctcgc gccagtcgcg aaagcctgct gccgccagct gaagcgcgtt ttcgatatcg
    11701 tcaacaccag cccacggcag cacagaaagt tgttcaccgg tggcaggatt tatcgaaatt
    11761 gcatgagttg ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga
    11821 tacgcagatt tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag
    11881 aatgctatgg atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt
    11941 acccaggctg cagcaaaagt gcatcgcgtg ccgtcgaatc tcactacccg tctacgccag
    12001 ctggaaacag aactgggggt tgatctgttt attcgcgaga atcagcgttt acgtctctcg
    12061 cctgccgggc ataacttttt acgctatagc caacaaattc tcacgttagt ggatgaagcg
    12121 cggagcgttg tcgctggcga tgagccgcaa ggtttatttt ctctgggatc gctggaaagc
    12181 accgctgcag tgcgcattcc ggccacgctg gcggaattta accgtcgtta tcccaaaatt
    12241 cagttttcac tttccaccgg cccttccggc actatgctgg aaggtgtgct ggagggaaaa
    12301 ctgaatgcgg cgtttattga tgggcccatt aaccatactg ccattgacgg gataccggta
    12361 taccgcgagg aactgatgat cgtcacgcca caaggacatg cgccagtaac ccgtgccagt
    12421 caggtgaatg gcagtaacat ttatgccttc cgcgccaatt gttcgtatcg tcgccacttc
    12481 gaaagctggt ttcatgctga cggtgccgct ccgggaacta ttcacgagat ggagtcttat
    12541 cacgggatgt tggcctgtgt ggtcgcagga gcaggcattg cgcttattcc acgctctatg
    12601 ctggaaagta tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt
    12661 tggttaacaa cctggctggt ctggcgtcgt ggtgcgaaaa cgcgtccgct tgaggcattt
    12721 attcaactgc tggatgcgcc tgactcggca aaacagggtt accaatgagc tatttttgat
    12781 agttcatgcg gctaatatgc tctatatagt gatgttccga tgacttatga ctatatgggg
    12841 caaatatggt tacgccagta agcatcagca attacatatc tcttcctgat gattttcccg
    12901 ttcgtaatat tgcacctcaa gtaaaagaag aacaggcccc cttcctgatc gtcattaaaa
    12961 tgtgatgtta acaactctct gatgcgtgac agaacaaccg tcagagagat ggaaatgcga
    13021 tccagatcac aaatgcattg tattcacatc attaaccgtt ttaagatcat ttcatcactt
    13081 tttcgcaact cacccgataa tctgttatga caacaaacac tgtttcccgc aaagtagcgt
    13141 ggctacgggt cgttacgctg gcagtcgccg cctttatctt caataccacc gaatttgtcc
    13201 ctgttggcct gctctctgac attgcgcata gttttcacat gcaaaccgct caggtcggca
    13261 tcatgttgac aatttatgca tgggtagtgg cgctaatgtc attgcctttt atgttaatga
    13321 ccagccaggt tgaacgccgc aaattactga tctgcctgtt tgtggtgttt attgccagcc
    13381 acgtactgtc gtttttatcg tggagtttta ccgttctggt gatcagtcgc attggtgtgg
    13441 cttttgcaca tgcgattttc tggtcgatta cggcgtctct ggcgatccgt atggctccag
    13501 ccgggaagcg agcacaggca ttgagtttaa ttgccaccgg tacagcactg gcaatggtat
    13561 taggtttacc tctcgggcgc attgtggggc agtattttgg ctggcgaatg accttcttcg
    13621 cgattggtat cggggcgctt attacccttt tgtgcctgat taagttactt cccttgctgc
    13681 ccagtgagca ttccggttca ttgaaaagcc tcccgctatt gttccgccgc ccggcattga
    13741 tgagcattta tttgttaact gtggtggttg tcaccgccca ttacacggca tacagctata
    13801 tcgagccttt tgtacaaaac attgcgggat tcagcgccaa ctttgccacg gcattactgt
    13861 tattactcgg tggtgcgggc attattggca gcgtgatttt cggtaaactg ggtaatcagt
    13921 atgcgtctgc gttggtgagt acagcgattg cgctattgct ggtgtgcctg gcactgctgc
    13981 tacctgcggc gaacagtgaa atacacctcg gggtgctgag tattttctgg gggatcgcga
    14041 tgatgatcat cgggcttggt atgcaggtta aagtgctggc gctggcacca gatgccaccg
    14101 acgtcgcgat ggcgctattc tccgggatat ttaatattgg aatcggcgcg ggtgcgttgg
    14161 taggtaatca ggtgagtctg cactggtcaa tgtcgatgat tggttatgtg ggcgcggtgc
    14221 ctgcttttgc tgcgttaata tggtcaatca tcatttttcg ccgctggcca gtgacactcg
    14281 aagaacagac gcaatagttg aaaggcccat tcgggccttt tttaatgata cgttttaatg
    14341 atttccagga tgccgttaat aataaactgc acgcccatac ataccagcag gaatcccatc
    14401 agacgggaga tcgcttcaat accccccttg cccaccagcc gcataattgc gccagagctg
    14461 cgtagacttc cccacaaaat aaccgccacc aggaaaaaga tcagcggcgg cgcaaccatc
    14521 agtacccaat cagcgaaggt tgaactctga cgcactgtgg aggccgagct aataatcatc
    14581 gcaatggttc ccggaccggc ggtacttggc attgccagtg gtacaaaagc aatattggca
    14641 ctgggttcat cttccagctc ttccgacttg cttttcgctt ccggtgaatc aatcgctttc
    14701 tgttgcggaa agagcatccg aaaaccgata aacgcgacga ttaagccgcc tgcaattcgc
    14761 agaccgggaa tcgaaatgcc aaatgtatcc atcaccagtt gcccggcgta atacgccacc
    14821 atcatgatgg caaatacgta caccgaggcc atcaacgact gacgattacg ttcggcactg
    14881 ttcatgttgc ctgccaggcc aagaaataac gcgacagttg ttaatgggtt agctaacggc
    14941 agtaacacca ccagccccag gcctattgct ttaaacaaat ctaacattgg tggttgttat
    15001 cctgtgtatc tggtttatca gcgaaaagta taaggggtaa acaaggataa agtgtcactc
    15061 tttagctaac cttgcatcgc attgaccaaa acttgaaccg atttagcaaa acgtggcatc
    15121 ggtcaattca ttcatttgac ttatacttgc ctgggcaata ttatcccctg caactaatta
    15181 cttgccaggg caactaatgt gaaaagtacc agcgatctgt tcaatgaaat tattccattg
    15241 ggtcgcttaa tccatatggt taatcagaag aaagatcgcc tgcttaacga gtatctgtct
    15301 ccgctggata ttaccgcggc acagtttaag gtgctctgct ctatccgctg cgcggcgtgt
    15361 attactccgg ttgaactgaa aaaagtgttg tcggtcgacc tgggggcact gacccgtatg
    15421 ctggatcgcc tggtctgtaa aggctgggtg gaaaggttgc cgaacccgaa tgataagcgc
    15481 ggcgtactgg taaaacttac caccagcggc gcggcaatat gtgaacaatg ccatcaatta
    15541 gttggccagg acctgcatca agaattaaca aaaaacctga cggcggacga agtggcaaca
    15601 cttgagcatt tgcttaagaa agtcctgccg taaacgaaaa aagaggtatg acgatgtcca
    15661 gacgcaatac tgacgctatt accattcata gcattttgga ctggatcgag gacaacctgg
    15721 aatcgccact gtcactggag aaagtgtcag agcgttcggg ttactccaaa tggcacctgc
    15781 aacggatgtt taaaaaagaa accggtcatt cattaggtca atacatccgt agccgtaaga
    15841 tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat ctggcagaac
    15901 gatatggctt cgagtcccaa caaactctga cccgaacctt caaaaattac tttgatgttc
    15961 cgccgcataa ataccggatg accaatatgc aaggtgaatc gcgtttttta catccattaa
    16021 atcattacaa caactagttg ataacgtgac aacgtcactg aggcaatcat gaaaccactt
    16081 ttatccgcaa tagcaactgc gcttattctc ttttctgcgc agggcgttgc ggaacaaacc
    16141 acgcagccgg ttgttacttc ctgtggcaat gtcgtggttg ttcccacatc gcaggaacaa
    16201 ccaccgtttg atttaaatca catgggtact ggcagtgata agtcggatgc gctcggcgtg
    16261 ccctattata atcaacacgc tatgtagtta ttgtcaggct ctgatcacag agcctgacat
    16321 ttccgtaata tcttcctgaa gtctcatata ttttcgccat tgttggcgat gagatcccga
    16381 taccagtaaa acgatgcttt acgtatccgt tccagcgttc ccttcccttc gttgtcttta
    16441 tcgacataaa tcattccgta gcgttttttc atttctcctg ttccggcaga aaccaggtca
    16501 atgcaacccc acggggtgta gccaattaag tcaacaccat cttcaactac ggcttttttc
    16561 atttcccgaa tatgggagga aaagtaatca atgcgatagt gatcgttcac cgtgccgtca
    16621 gcttgtctct ggtcaaccgc accaaatcca ttttcgacaa taaacagcgg caattggaaa
    16681 tgatcccaga accagttgag tgaataacgt agccctgccg gatcaatttg ccatccccat
    16741 tcggatgtat cgatataagg gttgctgacc aaatctcgtg gttcaacata atccagttgc
    16801 ggattatctg gcgaaaattg cgtcgtaaaa gacatgtagt agctaaagcc gataaagtcg
    16861 acacaacctc tggcaagaat cgcgttatca tctggtgtga tatcgaggtt gaatcctttc
    16921 cttgcaaagt aattcagcat atgttgcgga taatatccac gagcatgaac atcagtaaac
    16981 cagtaacgac gatgcatcgc tttcgtggcc atcatcatat cgttgggtgc acacgtcaga
    17041 ggatagatgg ggcacatagc aatcatacag ccgatattaa attcaggatt gatcagtttt
    17101 ccagtctgta ccgccagggc actggcaact aactcataat gtaccgcctg gtacattatt
    17161 tgttcgcgct cattgagatc ttcctctggc gaatacaaga taccggaatt agtaaatgga
    17221 cacaggcttt cgctgaaatt cacctgatta ttgatttcgt taaacgtcat ccagtacttt
    17281 actttttctt tatagcgcgt aaagaccgtt gatgcgaagt ggataaaaaa gtcgatcagt
    17341 ttacggtttc gccagccacc atattttgtc accagatgat aaggcatctc aaaatgcgaa
    17401 agcgtcacca caggttccat tccctgcttc aggcattcat cgaagagatc atcataaaat
    17461 tgtaaaccct cttcattcgg ctcctgttcg tcaccttgcg gaaagattcg tgtccaggca
    17521 atggaagttc gaaagcattt gaatcccatc ccggcaaata actgaatatc tgttttataa
    17581 cgatgataaa aatcaattgc ttcatgattg ggataattaa gtccgtcgat aacgccttct
    17641 gtcacttcac gcggcacccc gtgagcgcca gcagtcatta catcagcgat actgatgcct
    17701 tttcctcctt cattccagcc accttccaat tgatgtgcgg ctaccgcacc gccccataaa
    17761 aaatcttttt taaatcctga catacacaac tccttaacta aaatagatta ccaccagact
    17821 tccgcctgga tgccggccat aaactcgttt ttgctgttct cattaaactt aaaattcgat
    17881 aattcgttat ccagaatatt gatatacgtc ccgtagaagc gaatttctgg tcgtgaacct
    17941 aaaatacttt cacccacttt caatgcatgc cagagtgtcg ttttataagc cgattcattt
    18001 agcgttaccc cctgctgagt tttgttctgt tgcttaaacc agcctaattc aagccccgtc
    18061 tgattccatg tattccagat ccaggccggt cttattacgg tgcgaatact gtcaaaatca
    18121 ctatgagcgc cactttcata actataaaca tcttcgccat gagaatagac aagcgcgtta
    18181 gccataataa tattgtcagt cagatacatc tcgccctgag agattaaacg ccaggcgatc
    18241 ccattggtat ggtcaccata gtaatagcga ccatgcgcca tcgtattact ggcatctgag
    18301 aaactggcaa aactgctggc ataggaatta ttggcaacct gtaacgtaaa ttcattaaac
    18361 gtatcgcgtt gcaagttttg ccgtaaaaca gaagtcagca tccagctatc tttcatttca
    18421 aaataactgt cgtcattttc attatcttgt tgttgatccg ttttattagg tgcggaatat
    18481 ttagccatta atgacaacgt cgcatcatcc cataacggga tattgcgata acgaacatcc
    18541 acagaattgg tattcatctg actggtacgc gtaaaatcac gagagtaaac atcgacatca
    18601 tctcggctta aggacatatc aaacagccct acaccaagcg cccagttttc aatccccaca
    18661 cccgcggcaa catccgtggt taaggttttc cagtccagca tttggatctc atattgcggg
    18721 agtttatgtt tgccgaccca gaattctgcc tctcgtgcga agggtaaaaa acctcgcgtt
    18781 gtcagataaa tatcactgaa ctgcatgata ttttcattgg cactgtcacc aaaccaggca
    18841 tcgttatact gctgacctac gtttccatca taggtaacga ccgcatttgc cgttctaccg
    18901 tcctaaagaa gtaaccaatg gcacagttaa caatgggaac cataataaac ggcaccatca
    18961 tgatggggtt aagtacaata ggaaggccga acattatcgg ttcattgata ttgaaaatac
    19021 cggggataaa tgacattttt gccagatcac ggtattccgc acgcttagaa gcgataaaaa
    19081 tagccaacaa taaccccaac gtcatacctg aaccgccgca gttggcgaat gcatcattaa
    19141 tagaagtcca ggtaatcgga tatggcgcgc cccaggtagt gccgtgttga gcggcccagg
    19201 agagattctc cagattagcc tcagaaaaaa cggtttctcg aattgccgac gttgtattag
    19261 gtccgtggat ccccagcacc cagaggaaat tacccacagc ccccaggata attaccgcaa
    19321 agatattcgt tcccatatgt ttgagcggcg tctggattaa tgtgtaaatg agatcattta
    19381 atcctgccgg ggatatcaat gtcaggcaat aattaagtgc ggaaaagaac accatgacaa
    19441 aaaatattgg caataaaact ttaaatgaac gcgctaccgc aggaggtaca gctgccggca
    19501 tcgttatggt gattttgggg tttcgagcta agcgacaaaa tatttcactg gtgatcaatg
    19561 caacgataac agcaacaaac aacccctgcg cgccgagata tttggtcgtc aggaatgcct
    19621 gcccatctat catctggtat ggggtataaa caataaaaaa tgctcctatt gccgttaatc
    19681 cgcataaaag atcatcttgc tcatagctaa tcgccacatt cctggcgacc aggaaagcaa
    19741 tcataattga catgatattt acagaaccat tcattaccgg agtaaaaata gcttgtgctt
    19801 tttcaaggtt ggggaaaatg ctgttcagat gcagtaatcc ggcaataaaa ccgtcgggcg
    19861 ataatatggc aaagttaatt aatataatta atgagcttgc catgataatt ggaaacgtaa
    19921 aaacgaatcc atctctcacc gctgcaacat gtttttgtga gtttaacttg atagcaactg
    19981 gaacaagaaa acgttccatt ccacgttcga atgatgccat taatcccatt tttttatacc
    20041 tgttgtcctg gatatttata catcccaata aagcatgtat gccatatcca ttttatgatg
    20101 tgtgaaaagt tagttcaggt aatagtttaa taatattaga ttcttactct tgcagtagtt
    20161 tcatcgcaaa ttcaagaact gcctgtccgt tcattgtgcc atagtctttc atctcgataa
    20221 cggctacagg cattttcccc tgtgcaacgg cttcaatctc tggtttctga aagcgaactt
    20281 gcggaccaag taaaacaacg tcaacttcgt tatttttaat tttcccttta gcctctgcaa
    20341 tcgccaatgc ggaaatatta acttcaagtg aaatagcggt agcatgatca atcatacgtt
    20401 taaccagcat actggttgac atgcccgcag cgcaaactaa caatatcttt ttcatatttt
    20461 tccttactgg tatataacag actacattgt gtaaatatca tatcccacag tagctacacg
    20521 tcctggtaca ttgatcacag taatacagca ataaagttgt tcaacaacag ggtgataatt
    20581 aaacgactcg gccatacgat aaactaatca ggtctcaact ccttgttttc aatggctctt
    20641 aaaaggtgca aaagtgattt ttcaaaagat tgcccgctta cttaaatccg agatcaatgg
    20701 caattcatgg catgttggtg atttgttgcc gtcagaagcg gaactcgctg ttcgttataa
    20761 tgtttcgcgt aataccctgc gtaaggcatt atccctgctg gaaggcgaag gaattattca
    20821 cagaaagcat ggttcaggaa catacattca aaaaaagaat tttgttgcac acattgatca
    20881 catgaacagt ttcagtgaaa ttgcacataa aagcggcaaa gaggcaggaa gtcagattat
    20941 gaaatttgaa gtgcaggatg cttctcctac tattgcgact gagctgaatt tagtgactgg
    21001 tgagcaggtt tattacataa agagactgcg attcattgag gataatgcag cacaattaga
    21061 agaaacgtgg atgtcagtgg cacgttttcc tgatttaacc gtatcgcata tgcaaaaatc
    21121 caaattttcg tatattgaga acgaatgcgg gatcaaaatc attggcacct ttgaaacttt
    21181 ctccccgact tttcctaccc cagaaatcgc cagtatttta cggatcagcc cacgggatcc
    21241 catacttaaa attcagaccc aggctgtgga tagtaactct attccgctgg attattcgtt
    21301 actttacagc aatattttcg agttccaggt aaagtacttt ttcccgcgat aaaagcagac
    21361 gcacagagtg ctggcatcac attctgtgcg tatggtttag cctctgacct ttaccgcttt
    21421 acgccaccgc aagccaaata cattgatata cagcccggtc ataatgagca ccgcaccgaa
    21481 aaattgcaga cccgttaagc gttcatccaa caatagtgcc gcacttgcca gtccaactac
    21541 aggcaccagt aacgataacg gtgcaacccg ccaggtttca tagcgtccca gtaacgtccc
    21601 ccagatccca taaccaacaa ttgtcgccac aaatgccaga tacatcagag acaagatggt
    21661 ggtcatatcg atagtaacca gactgtgaat catggttgcg gaaccatcga gaatcagcga
    21721 ggcaacaaag aagggaatga ttgggattaa tgcgctccag attaccagcg acatcaccgc
    21781 cggacgcgtt gcgtgcgaca tgatcttttt attgaagatg ttgccacacg cccaactaaa
    21841 tgctgccgcc agggtcaaca taaagccgag catcgccaca tgctgaccgt tcagactatc
    21901 ttcgattaac accagtacgc caaaaatcgc taaggcgatc cccgccaatt gtttgccatg
    21961 tagtcgttca ccgaaagtaa acgcgccaag cacgatagta aaaaacgctt gtgcctgtaa
    22021 caccaacgag gccagtccag caggcatacc aaagttaata gcgcagaaca gaaaggcaaa
    22081 ctgcgcaaaa ctgatggtta atccataccc cagcagcaaa ttcagtggta ctttcggtcg
    22141 tgcgacaaaa aagatggccg ggaaagcaac cagcataaag cgcaaaccgg ccagcatcag
    22201 cggtggcatg ttatgaagcc ccactttgat gaccacaaaa tttagccccc atacaaccac
    22261 taccagtagc gccaacaccc catcttttcg cgacattcta ccgcctctga atttcatctt
    22321 ttgtaagcaa tcaacttagc tgaatttact tttctttaac agttgattcg ttagtcaccg
    22381 tttacgacgg cattaatgcg caaataagtc gctatacttc ggatttttgc catgctattc
    22441 ctttacatct ctaaaacaaa acataacgaa acgcactgcc ggacagacaa atgaacttat
    22501 ccctacgacg ctctaccagc gcccttcttg cctcgtcgtt gttattaacc atcggacgcg
    22561 gcgctacgct gccgtttatg accatttact tgagtcgcca gtacagcctg agtgtcgatc
    22621 taatcggtta tgcgatgaca attgcgctca ctattggcgt cgtttttagc ctcggttttg
    22681 gtatcctggc ggataagttc gacaagaaac gctatatgtt actggcaatt accgccttcg
    22741 ctagcggttt tattgccatt cctttagtga ataacgtgac gctggtggtg ctcttttttg
    22801 cccttattaa ctgcgcctac tccgtttttg ctaccgtgct gaaagcctgg tttgccgaca
    22861 atctttcgtc caccagcaaa acgaagattt tctcaatcaa ctacaccatg ctcaacattg
    22921 gctggaccat cggtccgcca ctcggcacgc tgttggtgat gcagagcatc aatctgccct
    22981 tctggctggc agctatctgt tccgcgttcc ccatgctttt cattcaaatt tgggtaaagc
    23041 gcagcgagaa aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa gttttattac
    23101 aagataaagc actgttgtgg tttacctgct ctggctttct ggcttctttt gttagcggcg
    23161 catttgcttc atgcatttca caatatgtga tggtgattgc tgatggtgat tttgccgaaa
    23221 aggtggtcgc ggttgttctt ccggtgaatg ctgccatggt ggttacgttg caatattccg
    23281 tgggccgccg ccttaacccg gctaacatcc gcgcgctgat gacagcaggc accctctgtt
    23341 tcgtcatcgg gctggtcggt tttatttttt ccggcaacag cctgctattg tggagtatgt
    23401 cagctgcggt atttactgtc ggtgaaatca tttatgcgcc gggcgagtat atgttgattg
    23461 accatattgc gccgccagga atgaaagcca gctatttttc cgcccagtct ttaggctggc
    23521 ttggtgctgc gattaaccca ttagtgagtg gcatagtact taccagtctg ccgcctttct
    23581 cgctgtttat catcttagcg ttggtgatcg ttgttgcgtg ggtgctgatg ttaaaaggaa
    23641 ttcgtgcaag accgtggggg cagcccgcgc tttgttgatt taagtcgaac acaataagaa
    23701 tttaattcag ccttcgttta ggttacctct gctaatatct ttctcattga gatgaaaatt
    23761 aaggtaagcg aggaaacaca ccacaccata aacggaggca aataatgctg ggtaatatga
    23821 atgtttttat ggccgtactg ggaataattt tattttctgg gtttctggcc gcgtatttca
    23881 gccacaaatg ggatgactaa tgaacggaga taatccctca cctaaccggc cccttgttac
    23941 agttgtgtac aaggggcctg atttttatga cggcgaaaaa aaaccgccag taaaccggcg
    24001 gtgaatgctt gcatggatag atttgtgttt tgcttttacg ctaacaggca ttttcctgca
    24061 ctgataacgg atcgttgaca cagtagcatc agttttctca atgaatgtta aacggagctt
    24121 aaactcggtt aatcacattt tgttcgtcaa taaacatgca gcgattcctt ccggtttgct
    24181 taccctcata cattgccctg tccgctcttc caatgaccac atccagaggc tcttcaggaa
    24241 atgcacgact cacaccagca gtcacggtaa tgttgatatg cccttcagaa tgtgtgatgg
    24301 catggttatc gactaactgg caaattctga cacctgcacg acatgcttct tcatcattag
    24361 tcgctttgac aataatgata aattcttcgc ccccgtagcg ataaaccgtt tcgtaatcgc
    24421 gcgtccaact ggctaagtaa gttgcgaggg tgcgtaatac tacatcgccg attaaatgcc
    24481 cgtaggtatc attaaccaat ttaaatcggt ctatatccaa caacattaaa taaagattca
    24541 gaggttcagc attgcgtaac tgatgatcga aggattcatc aagaacccga cgacccggca
    24601 atcccgtcag aacatccata ctgctacgga tcgtcagcaa ataaattttg taatcggtta
    24661 atgccgcagt aaaagaaagc aacccttcct gaaaagcgtc gaaatgcgcg tcctgccagt
    24721 gattttcaac aatagccagc attaattccc taccacagtt atgcatatgt tgatgggcag
    24781 aatccattag ccgaacgtaa ggtaattcat cgttatcgag tggccccaga tgatcaatcc
    24841 accgaccaaa ctggcacagc ccataagaat ggttatccgt tatttctggc ttactggcat
    24901 ctctcgcgac cacgctgtga aacatactta ccagccactg gtagtgggca tcgatagcct
    24961 cattgagatt taacaagatg gcatcaattt ccgttgtctt cttgatcatt gccactcctt
    25021 tttcacagtt ccttgtgtgc gctattctaa cgagagaaaa gcaaaattac gtcaatattt
    25081 tcatagaaat ccggagttat gagcatctct gagataacat tgtgatttaa aacaaaatca
    25141 gcagataaaa aagtatttaa ttct
//
LOCUS       DADTFG010000065        25083 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.065, whole genome shotgun sequence.
ACCESSION   DADTFG010000065 DADTFG010000000
VERSION     DADTFG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.065"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(105..767)
                     /gene="dedD"
                     /locus_tag="J1138_002445"
     CDS             complement(105..767)
                     /gene="dedD"
                     /locus_tag="J1138_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBA8454228.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(757..2025)
                     /gene="folC"
                     /locus_tag="J1138_002446"
     CDS             complement(757..2025)
                     /gene="folC"
                     /locus_tag="J1138_002446"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBA8454229.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(2095..3009)
                     /gene="accD"
                     /locus_tag="J1138_002447"
     CDS             complement(2095..3009)
                     /gene="accD"
                     /locus_tag="J1138_002447"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBA8454230.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(3165..3824)
                     /locus_tag="J1138_002448"
     CDS             complement(3165..3824)
                     /locus_tag="J1138_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA8454231.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(3907..4719)
                     /gene="truA"
                     /locus_tag="J1138_002449"
     CDS             complement(3907..4719)
                     /gene="truA"
                     /locus_tag="J1138_002449"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBA8454232.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(4719..5732)
                     /locus_tag="J1138_002450"
     CDS             complement(4719..5732)
                     /locus_tag="J1138_002450"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBA8454233.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATATWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCIRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(5798..6934)
                     /gene="pdxB"
                     /locus_tag="J1138_002451"
     CDS             complement(5798..6934)
                     /gene="pdxB"
                     /locus_tag="J1138_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBA8454234.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLSEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            7033..8028
                     /locus_tag="J1138_002452"
     CDS             7033..8028
                     /locus_tag="J1138_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBA8454235.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(8025..9203)
                     /locus_tag="J1138_002453"
     CDS             complement(8025..9203)
                     /locus_tag="J1138_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8454236.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATMFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(9468..10688)
                     /gene="fabB"
                     /locus_tag="J1138_002454"
     CDS             complement(9468..10688)
                     /gene="fabB"
                     /locus_tag="J1138_002454"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBA8454237.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            10847..12853
                     /gene="mnmC"
                     /locus_tag="J1138_002455"
     CDS             10847..12853
                     /gene="mnmC"
                     /locus_tag="J1138_002455"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBA8454238.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLARADLVLAHQHWPELAPWAEQLQAQWPLPLSGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGITQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAEQKGLQIHYQHQLQDLSRKDDGWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPHAQWAKEIDVSDKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWMRKLLKGKAVKAG"
     gene            complement(12974..13252)
                     /locus_tag="J1138_002456"
     CDS             complement(12974..13252)
                     /locus_tag="J1138_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBA8454239.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(13286..13834)
                     /locus_tag="J1138_002457"
     CDS             complement(13286..13834)
                     /locus_tag="J1138_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBA8454240.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(13834..14643)
                     /locus_tag="J1138_002458"
     CDS             complement(13834..14643)
                     /locus_tag="J1138_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBA8454241.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(14643..15467)
                     /gene="mepA"
                     /locus_tag="J1138_002459"
     CDS             complement(14643..15467)
                     /gene="mepA"
                     /locus_tag="J1138_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBA8454242.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVVFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(15471..16556)
                     /gene="aroC"
                     /locus_tag="J1138_002460"
     CDS             complement(15471..16556)
                     /gene="aroC"
                     /locus_tag="J1138_002460"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBA8454243.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGASIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(16591..17523)
                     /gene="prmB"
                     /locus_tag="J1138_002461"
     CDS             complement(16591..17523)
                     /gene="prmB"
                     /locus_tag="J1138_002461"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBA8454244.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            17689..18240
                     /gene="smrB"
                     /locus_tag="J1138_002462"
     CDS             17689..18240
                     /gene="smrB"
                     /locus_tag="J1138_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBA8454245.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(18564..19406)
                     /locus_tag="J1138_002463"
     CDS             complement(18564..19406)
                     /locus_tag="J1138_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBA8454246.1"
                     /translation="MKIFRWLCCLVMLTCAAESLAAKKIATLVTPVAYSGYLTQIRYY
                     LEILTPEGVEYGVYKQHSIVLQAENLDLVSWTGSTAAPTLKMEELNDASKSTCVGLDA
                     YDARSSKSDWICYNASLGVYYDGEVHGCPWLISTYTDSNISSVDGVGLYIGPKVHNSS
                     CPVSVAAYDISWSEDYVVHSKVLSLQSTGGTIEKTLPTFLMESGKLCDGSVMDDRGAY
                     CRFVAQMITFSTSGCDSSKVTVTPNQHPITDKQLHDMVVRVDTSSRQPIDSTCRFQYT
                     LNEL"
     gene            complement(19408..19935)
                     /locus_tag="J1138_002464"
     CDS             complement(19408..19935)
                     /locus_tag="J1138_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8454247.1"
                     /translation="MNLKRISHCAAALAAVLLTPHVQAADNLHFYGNLLSKSCTLVVQ
                     GESLAEVHFPTISRKDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDASSAAQGVGIGMEKTDGTPVPINNTSGATFVLSNGNNNLNFNAWLQAKSGREVT
                     MGDFTASLTATFEYI"
     gene            complement(19932..20411)
                     /locus_tag="J1138_002465"
     CDS             complement(19932..20411)
                     /locus_tag="J1138_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8454248.1"
                     /translation="MKRLAWCLIYGFAGLAQAAINDVTFHGTLVSPPACTISDGKTIE
                     VEFRNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGSQTPYDDSAIETDVS
                     GLGIELQQNGQPFKLGTPLKIDPSTPPTLQAVPMKANDAALSEGTFSAYATLQVDYQ"
     gene            complement(20408..20911)
                     /locus_tag="J1138_002466"
     CDS             complement(20408..20911)
                     /locus_tag="J1138_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8454249.1"
                     /translation="MKAFFLTGSAALLLACGAIVSPVMAGGKIVNLTLRVLVDGPPPC
                     SVKGSAVEFGNVIIKSIDGANYRQPVGYTLNCSNSVSDDLQMQLQATTTTINGETVLS
                     TGISGFGIRIQNAADHSLVAVGNSSWLPFNINTQPDFEAVPVKQSGVQLMASEFNATM
                     TMVVDYQ"
     gene            complement(20928..21680)
                     /locus_tag="J1138_002467"
     CDS             complement(20928..21680)
                     /locus_tag="J1138_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA8454250.1"
                     /translation="MSYKLSCPMLVSTALMALLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRRPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKLASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            complement(21700..24348)
                     /locus_tag="J1138_002468"
     CDS             complement(21700..24348)
                     /locus_tag="J1138_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBA8454251.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKNYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDDDDSSNSTTSKHWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVNTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADKHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKE
                     MYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVG
                     TSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGVNAGEHMMVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAP
                     EIKPVIQDQTRQVTPTEAPTSISATQ"
     gene            complement(24429..24995)
                     /locus_tag="J1138_002469"
                     /pseudo
     CDS             complement(24429..24995)
                     /locus_tag="J1138_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         5848 a         6983 c         6456 g         5796 t
ORIGIN      
        1 aggcaacggc tgtcggatgc ggcgtgaacg ccttatccga cctacggctc tacccctgca
       61 caggcctgat aagatgcgcc agcgtcgcat caggcaagac cgtattaatt cggtgtatag
      121 cccattacca cgccacttaa gccagaaagc tgcttgagct cacccagcga tcctttcagc
      181 ttctctttgg aagcatccgg cccaaccaga atacgggtaa ttttaccctg cactggcgtg
      241 gatggcgacg tataaacccg ataaccggca ccgcgcagct taccgacaat ctcattcact
      301 ttatcggcat ttttcagcgc gcccagttgc acaacataag ctttacccgt tggcgctgct
      361 ttttcttcca cgaccggctt cggttctggc ttcggcgcag gtggtgcttc aacctttggc
      421 ttaagcggct ccaccggttt cggcttcggt ggggcgaccg gtgcaggttc cggttcaaac
      481 tcggtgttat tggctgcaat agtggccgga tcgagcgacg gtgctgcggc atcacctgcc
      541 cgtacctctt ccgctgcgcc ttccggcggc tgcgtcggca acgcctgggt ggcggctggc
      601 atcatatcag gctcatcgcg atcgcccgct tttggcacca gcgggatagc cgcgaactca
      661 tcctgataat gttttttctg cccgtccagc agccctggaa gtacaatcac ccccagcgct
      721 accagcacga tcgtgcccac taaccgattc tgaaacttac ttgccaccgc ttctcctcgc
      781 gtcaatcact tccatgacat gtgcgaccgt gtggaacgaa ccacacacca gcacggtgtc
      841 ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc gcaacgctat caaatgattt
      901 gccgttaccc aaatgctcaa gcagttgttc tgccgtggca ccgcgcggcc cttccagtgg
      961 cgcacaatac cagtcatcaa ccacgctttt caaccaggcc agagttccgg caatatcttt
     1021 atcatgtagc ataccgataa ccgccagcac gcgcccgttt ttcggtagcg ctttcatacg
     1081 cccggtgaga tattccgccg catgtggatt atgcgcgaca tcaaaaataa cgcgtggcga
     1141 ctcgctcaca atctggaaac gtcccggcaa aattgcgctg gcaatcccgt cgcgaatggc
     1201 attttcactg acctccagcc cgctggcacg cagtgccgcc agcgccgttg cggcattcgg
     1261 ttgcgggaca agcggcaacg gcagattttc cagcgtgccg tgagcatcgc taaacgtcca
     1321 gtcatgatcg gtgacggaat agttccactc aacgccccga cgttgtagca gtgcaccttt
     1381 ttcctgcgcc acatcagcaa tggtagaagg catttccggc tcaccgacaa ttgccggttt
     1441 ttcgctgcga aagatacctg ctttctcgcg accaatactt tcgcgatctg gccccagcca
     1501 gtcggtatga tccagcgcaa tactggttac taccgcgaca tcggcatcga caatattggt
     1561 tgcgtccaga cgaccgccca gccctacttc cagaatcact acgtcaagtt gtgcctgctt
     1621 gaacagccac aacgccgaga gcgtaccgta ttcgaaatag gtcagggaaa tatcaccgcg
     1681 tgccgattca atctccgcaa aagaagcggt gtgggccgat tccggcaatt cctgaccctg
     1741 cacacgtacg cgctcggtat aacgcaccag atgcggcgaa ctgtagacgc ccactttgta
     1801 ccctgccgcc atcagaatcg actccagcgt acggcaggtg gtgcctttgc cattcgtacc
     1861 cgcaacggta aacacaaatg gcgctggttt cagaacgcca agacgcgccg cgacctggct
     1921 cacgcgctca aggccgagat cgatagtttt actgtgcagg ttttccagat aagaaagcca
     1981 cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata atcatggtat ccgctgattc
     2041 gttacggtga gaatagcaaa agggcagagc cagtggccct gcccttatca gttatcaggc
     2101 ctcaggttcc tgatccggta ccggtggcac cactacgcct tcacgcggcg cttcaggatt
     2161 cggcgctggc agattcatca acttcgccag aatgctcgcc agtttcaggc gcatttccgg
     2221 acgacggacg atcatgtcga tcgcgccttt ctcgatcagg aattcactgc gctggaatcc
     2281 tggcggcagt ttttcgcgaa cggtctgttc gataacacgc ggaccggcaa agccaattaa
     2341 cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg aaacttgcag aaacaccgcc
     2401 catggtcggg tcggtcagca cggagatgta cggcaagccg cgctcctgca tttttgccag
     2461 tgccgcagag gttttcgcca tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc
     2521 accagaggcg gagaagcaga tcagtgggca gttatcttcc agcgcctgct caacggcacg
     2581 cacgaaacgc gcacctacca cagatcccat ggaaccgccc ataaaggcga actcgaatgc
     2641 cgcagcgaca accggcattc catacagggt gcctttcatc accaccagcg catctttttc
     2701 gccggtttct ttctgcgcag atgccagacg gtctttatac ttcttggagt cacgaaactt
     2761 cagcacatct ttcggctcaa gctcgctacc cagctccaca aggcttcctt catctaacag
     2821 gctatgcagg cgattacgcg ctgtcatacg catgtgatgg tcacacttcg gacagacctc
     2881 aagattacgt tccagctcag cgcggtataa aacctgaccg cagctatcac acttagtcca
     2941 caccccttca ggaatgctcg ccttgcgggt gggagtaatg ttgcttttaa ttcgttcaat
     3001 ccagctcatt agggaccttt ctgtctgaac ctggttcgat gccagtttta tctttgggga
     3061 cgcataatgc catttttgcc cccaacagac catgaatgtt gcacattaaa acataacagc
     3121 ccgaaacttt ggataaaaaa gtggtcgaac cgcagagtta ctttttattt tgcggcgcgt
     3181 gccgctgcgc gtttgtggcg aataaattcg aaaacacctg gcaatattga aacaaaaata
     3241 atgccaacaa tcatcagttt aaggttgctc tgaatgaagg ggagcgtgcc gaagaaatag
     3301 cctgcgtagg taaaaagcag tacccacaac agtgcgccaa tcacgttata agcggcgaaa
     3361 tgacggtacg acatgtggcc cattcccgca acaaacgggg cgaacgttct gacgatcggc
     3421 acaaaacggg cgagaataat cgttttgccg ccatgtttct cataaaactg atgggttttg
     3481 tcgagataac tgcgacggaa aattttcgaa ttgggattac tgaataactt ttcaccgaac
     3541 aaccgcccga tagtgtaatt gaccgcgtca cccacaatcg cggcgatcag catcagcacc
     3601 accatcatat ggacgttgag atcgttggtc tccagtgatg ccaacgctcc ggcaacaaac
     3661 agcaatgaat cacccggtaa aaacggcgtt accaccagac cggtttcaca gaacaaaatt
     3721 aagaacaaaa tggcataaac ccagacgccg tactccgcga ccagctctgc cagatgcacg
     3781 tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt ccatatttgc ctatgccttg
     3841 tactcgtcat ctatcaggct gtaactacgt tggctacagc ctgaaatgtt ccgagcatta
     3901 ttctcgttag tccgccagaa atagcgggcc cattggcggt tttggaagat catatcggtc
     3961 agggtaatcc accgcgacca gatatagccc ttccgctttt gccgttgctg ccgccagcgt
     4021 tctgtccttt gccgccagca actctgctat ccagctctcc ggctggttgt gggcaccgac
     4081 ttccatcagg ctgccgacaa tattcctgac catatgatgt acaaaggcat tcgctttgat
     4141 atctaccacc acataaggac cgtgacgcgt gacattaata tgcataacgt tgcgccacgg
     4201 ggtccgggac tggcactgca ccgcacggaa cgaggtgaaa tcattctcgc ccagcaagca
     4261 ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg taaaaatggg ttaccccttt
     4321 actcaatacc gccgggcgca gccgatgatt gtagatgatg tagcgataac ggcgagccgt
     4381 ggcgctaaat cgggcatgaa aatcatcagg tacagcttta acccaacgca cggcgatgtc
     4441 accaggtaaa ttcgcattta cgcccaacgt ccacgcagcg tctttgcgct gcgcggtcgt
     4501 ttcgaaatgc acaacctgcc cggtgccgtg tacccctgcg tcagtacgcc cggcgcagaa
     4561 gacggtgatc ggttcgttcg ccacctggga gagcgccttt tccagcttct cctgcacact
     4621 gcggacttcg ttctgccgtt gccagccgta atacttactg ccgtcgtact caatgcccag
     4681 cgcaatttta taaactggcg gttgttgctg gtcggacatt agtacagata ctcctgcacc
     4741 agtttctcgg cgatttttac tgccatcagc gcgccgccaa agcgaacgtt atcggccacc
     4801 gaccagaact gaacttgctc cggcataccg tagtcattac gtatgcagcc aacggaaaga
     4861 tgcggcgtac cagaagcatc tcctacctga gtcgggaatt cgttctcttc agagagcaca
     4921 atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg ccgccagcgg acgcagtgct
     4981 tcaaagttga ccatctgggc atgaccgtag aataccggtg cctggacgac gcttgccgaa
     5041 atcatcagcc cttcgtcctg caggattttg cgtacttcgt caacgatacg acgttcttca
     5101 cgcacgctac cttcgctatc cggcagtaac ggcagcatgt tgaacgccag ctgacgcccg
     5161 aagaaatctt cttcgtcaat cggaatgcca ttgagtaatt tagcactctg ccccgctaac
     5221 gcatcgaccg cttttttgcc ctgggcggag gctgaaatca ggctggtaac gctgatacgt
     5281 gataaaccgc cctgatcaat tagcggtttc agtgccgcca gcagctggct ggtcagactg
     5341 tctggtacgg cgatgacatt ccggttgcgg taatcggtca gtacaaacgg gtttacttcc
     5401 ggcaccacca gcggtacgtc gggttcgaga gcaaacaatc cgctgctgtc gatcaccaga
     5461 caacctgagt tggttgcttc ttcaacccag gtagcggtag cttctttgcc tgcgacaaaa
     5521 aacgccagct gcgcctgcgt ccagtcgaat tcagcggcat cctgcacggt gattgtctta
     5581 ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg ccagtgcata aatttcccca
     5641 accgggaact gacgttcagc cagcgtttca agcagggctt cgcccacagc gccagttgcg
     5701 cccaggacgg caatgttcca gccttcagac atggtggttt actccagaaa tagcaaagct
     5761 ccctgccaat gttacagcag agagcatgaa gaagagatta acgtgccgga tgatgaacgg
     5821 cgttaaaacc cagtttacac agcaatgatg ccgcactggc gtcatcacaa atcacataca
     5881 gagatgacca ttcacggcgc tcaagatagt ttttgcgcag tttatcgaac tcacccggta
     5941 tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac atcatacacc aaatgcacca
     6001 gccttttcag cgttggctga tcgagcgggc catgcagcgt aatgcgacca aactctggcg
     6061 cgggcagtaa tgtatccagc gcaacgtgct gttcatgccc aataaacttg ctataagctt
     6121 caaacacttg cgtagtaccg cgtgctttac cttccagggt atagcctgcg atatgcggcg
     6181 tgccgatatc cacttttttc agcaattcca cgttaagttc cggttcgcct tcccagacat
     6241 ccagcactac gcttaacttc tggccttcgc tcaggcaggt cagcagggcg gtattatcga
     6301 cgactgcgcc acggcaggcg ttaatcagga tcgctcccgg tttcaggctg cggatcagtt
     6361 tttcatccgc cagatgcagc gttttgtacg gaccatcttt aaagagtggc gtatggaaag
     6421 tcagaatatc cgcatgctga actaattcat ccagcgagcg gaaatccccc tcatccccac
     6481 ggtcggcgcg gggcggatcg caaagtaagg ttttgatccc taaagcttcc agtcgcgcct
     6541 gtaaacgacg accaacgtta ccaacgccca cgatccccac ggtacgctcg tgcagtgaaa
     6601 atccatcgcg ttcggcaagc atcagcaggg aggagaaaac atattccact accgcaatcg
     6661 cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg ctttaaccat gcttcatcga
     6721 catggtcggt ccccgcggtg gcagtgccaa caaatttaat gggttttcct gccagcaaag
     6781 attcattcac tttcgtgacc gaacgcacca taagcgcatc cgcgtcggcc agttgagcga
     6841 cgggaattgg acgcccggga accgcggtca cctcacccaa acggctaaat aagtcgcggg
     6901 cataaggcat attttcatca acaaggattt tcacgtttgt gttacctgta tgagacgaga
     6961 gttaaccaga caagtgtgcc ataatctcgc ggccaggcat acttgcgaag atttcaggta
     7021 taaggatacg taatgataca acctatttcc ggccctcctc ctgggcaacc accaggtcag
     7081 ggagataacc tgccgtctgg cgcgggcaat cagcctttat ccagtcagca acgtacttcg
     7141 ctggaaagct taatgacgaa agtgacctca ctgacgcaac aacaaagagc agaactgtgg
     7201 gcgggtatca ggcacgatat tggtctgtcg ggagattcac cgctgctttc gcgtcacttc
     7261 cctgccgctg agcataatct ggcgcaacgt ctgttggccg cgcaaaaaag ccattctgcc
     7321 cgccagcttt tagcgcaatt aggggagtat ttacgtctgg ggaataatcg tcaggcggtc
     7381 acggattata tccgtcataa ctttggtcag acgccgctga atcagctctc accggagcaa
     7441 ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg ttattccgca accacagcag
     7501 cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca atgcgctcaa acagctggtg
     7561 accaaacttg cggcagcaac gggggaaccc agcaaacaga tctggcaatc gatgctggaa
     7621 ctttccgggg tgaaagatgg cgagttaatt ccagcgaaac tgtttaacca tctggtgacc
     7681 tggctacagg cgcgtcagac gctaagccag caaaatacgc cgacgcttga atcactacag
     7741 atggcgctga aacaaccttt agatgccagt gaactggcgg cgttatcggc atatatccag
     7801 caaaaatatg gcctttctgc gcaatcatcg ctttcttctg cccaggccga ggatattctt
     7861 aaccagcttt atcaacggcg ggttaaaggg attgagccgc gtgatatgca accgctgctt
     7921 aatccttttc caccgatgat ggacacgttg caaaatatgg caacgcgtcc cgcgctgtgg
     7981 atactgttag tcgcgattat cctgatgctg gtctggctgg ttcgttaacc ccgacgaaac
     8041 gacagtatcg tgacaataat tcccagcacc gcagagatcg ccccggcaag aaataccgaa
     8101 gagtaaccaa acatggtcgc cagcattccc gcaagcggcc cggagacgcc gagggcgata
     8161 tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa cgtgtgaggg gacgcgttta
     8221 accacctcca cgcccagcgc aggaaagata agcgaacatc cggctccagt taacgccgcg
     8281 cccgctaatg cgacccacgc ccctggggct tgccagagca gcaacaagcc caccgtttct
     8341 acaagcagag agacgatcgc cactttcacg ccgccaaaac ggtccggcat ccagccaaac
     8401 atgacgcgca tcacgacaaa tgcgccgcca aacgcggtaa gagtaaagcc cgccatcgcc
     8461 catcctttgc tggcaaagta gagcgaaacg aaagtcccga taaccgcaaa accaacgcct
     8521 tgtagtgcca gacctaaccc tggtttccag ataagcccga caacgctcca cagcgatgga
     8581 cgttcccccg ccagggccgg tactttgcgc actgtgccgt tacaggccca cgccagtaag
     8641 ggtaatgcca ttgtggtgag cgccagtgcg gcaaaaccgt aatggctatg aatcaacagg
     8701 ccaagcggcg caccaacagc gagggcaccg taaatagcca ttccgttcca tgacatcact
     8761 ttgccggaat gttttggccc aacgatgcct aatccccagg tcagagcgcc cgtcagtaac
     8821 tggctttcac caaagccaag aatcaaacgc ccgacgacca acagagcaaa tttgaacggt
     8881 gcggggacgg gcaaaatcgc cgccagcagc aacgcgcccc ccgccagacc acaagctaac
     8941 atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg ccagtcgccc ggcgtaacca
     9001 cgcgtcagca ccgtagccag aaactgaatc ccgacggcaa tgccgaccat agtgttgcca
     9061 tagcccagtt catgatgaac aaacagcggg ataaccggca acggtaaacc tacggtcatg
     9121 taggtgagaa aaaccgcaaa agcgatgcgg aagagcgaaa aattggcaga agatcgtgtt
     9181 tcggtttggc ttacagcagt catgcattac tccagaatgc agcgcaaggc gaggagtatc
     9241 cccgtctcat ctctctggtt tcagggttac ggtgcgttgg caggatttaa cgcgtacgtc
     9301 ttttctgcag gaaatcgaca aagcgggaag tttgcctgga actggcggcg attgtcaatg
     9361 atgtgaaaaa gggaaccatc gggttccctt ttgcgttagt gccggaggcc gcattggcgc
     9421 gtaacgtcgg atgcggcgct ggcgcgtctt atccgaccta ctccgaatta atctttcagc
     9481 ttacgcatta ccagcgtggc gttggtgccg ccgaagccga agctgttaga cataacggtg
     9541 gtcagttcgc gatcggtcgt ttcggtcacg atgttcagac ccgcagcctg ctcgtccagc
     9601 tcttcaatgt tgatgctcgg ggcgataaaa ccgtgttcca gcatcagcag agagtagata
     9661 gcttcctgta cgccagcagc gcccagtgag tgaccggtca ttgctttggt tgcagaaatc
     9721 gccgggctct tatcgccgaa cacttcacgg atagctgcca gttctttcac gtcgccaacc
     9781 ggagtcgaag taccatgaga gttcaggtag tcgattgggg tatcaacacc gtgcatcgcc
     9841 atcttcatgc agcgcactgc accttcgcca gacggagcaa ccatgtctgc gccatcagag
     9901 gttgcgccgt agccaacgat ttcagcatag atgtgagcac cacgcgccag cgcgtgttcc
     9961 agctcttcaa ccactaccat accgccaccg ccagcgataa cgaaaccgtc acggtgagcg
    10021 tcgtaagtac gggaggcttt ttccggggtg tcgttgtatt tagtagacag cgcacccatc
    10081 gcgtcgaatt cgcaagccat ttcccagcac agctcttcgc cgccgccagc aaacacgatg
    10141 tcctgtttgc ccagttggat ctgctctact gcgttaccga tacagtgtgc ggaagtcgca
    10201 cacgcggagc taatggagta gttaacgccg tgaattttaa acggcgtggc gaggcaggca
    10261 gaaacgccgg atgccatcgc tttggtgacc acatacgggc caaccgcttt caggccgcgc
    10321 gggccgcgca ttgcgtcagc gccgaaaacc tggaaacgcg gggagccgcc gccggaacct
    10381 gcaatcaggc caacgcgcgg gttattctgg taagcttccg gggagaggcc agcatctgca
    10441 attgcctgct ccatagaaag gaatgcataa atggatgcgt cgctcataaa gcgcacaact
    10501 ttgcggtcaa tgaggccagt ggtatccagt tttacgttgc cccagacgtg gctacgcatg
    10561 ccggaatcct tcagctcctg agagaaagtg atccctgaac gtccttcacg cagagatgcc
    10621 aggacttcct gctggttatt accgatgctg gaaacaatgc ccaggccagt aatcactgca
    10681 cgtttcattc aatacctctg taagtcgcac atagagtaag tttcgaatgc acaatagcgt
    10741 acacttgtac gccgaacaag tccgatcagc caattaatag agaaatttgc gcagcctgac
    10801 acacatcgct aagatcgagc caccgcctgt aagacgagta acttacgtga aacactactc
    10861 catacaacct gccaacctcg aatttaatgc tgagggtaca cctgtttccc gagattttga
    10921 cgatgtctat ttttccaacg ataacggact ggaagagacg cgttatgttt ttctcggggg
    10981 taaccattta gaggcacgtt ttcctgaaca tccacatcct ctgtttgtgg tagcagagag
    11041 cggcttcggc accggattaa acttcctgac gctatggcag gcatttgatc agtttcgcga
    11101 agcgcatcca caggcgcaat tacaacgctt acatttcatt agttttgaga aatttcccct
    11161 cgcccgtgcg gatttagtct tagcgcatca acactggccg gagctggcgc cgtgggcaga
    11221 acaacttcag gcacaatggc ctttgcccct gtccggttgc catcgtttat tgctcgatga
    11281 aggccgcgta acgctggatt tatggtttgg cgatataaac gaactgacca gccaactgga
    11341 cgattcgcta aatcaaaaag tagatgcctg gtttctggac ggctttgcgc cagcgaaaaa
    11401 cccggatatg tggacacaaa atctgtttaa cgccatggcg cgactggcgc gtccgggcag
    11461 cacgctggcg acctttacgt ctgccggttt tgtccgccgc ggtttgcagg aagccggatt
    11521 cactatgcaa aaacgtaagg gctttgggcg caaacgggaa atgctttgtg gagtgatgga
    11581 acagacatta cccctcccct gctccacgcc gtggtttaac cgtacgggca gcaacaaaca
    11641 ggaagcggca attattggag gcggtattgc cagcgcgttg ttgtcactgg cgctattgcg
    11701 tcgcggctgg caggtaacgc tttattgcgc ggatgaggct cccgcactgg gcgcttccgg
    11761 caatcgccag ggggcgctgt atccgctttt aagtaaacac gatgaagcgc taaaccgttt
    11821 tttctctaat gcgtttactt ttgctcgtcg gttttacgat ttattgcccg ttaaatttga
    11881 tcatgactgg tgcggcatca cgcagttagg ctgggatgaa aaaagccagc ataaaatcgc
    11941 acagatgttg tcgatggatt tacccgcaga actggctgta gccgttgagg ctaatgcggt
    12001 tgaacaaatt acgggcgttg caacaaattg cagcggtatt acttatccgc aaggtggctg
    12061 gctgtgtccg gcggaactga cccgtaatgt gctggaactg gcggaacaga aaggtttgca
    12121 gattcattat caacatcagt tacaggattt atcccgtaag gatgacggtt ggttgttgaa
    12181 ttttgcagga gatcagcaag caacacacag tgtagtggta ctggcgaacg ggcatcaaat
    12241 cagccgtttc agccaaacgt catctctgcc agtatattcg gtggccggtc aagtcagcca
    12301 tattccgaca acgccggaac tggcggagct gaagcaggtg ctgtgctatg acggttatct
    12361 cacgccacaa aatccggcga atcagcacca ttgtattggt gccagttatc atcgcggcag
    12421 cgaagagacg gcgtacagtg atgaagatca gcagcagaat cgccagcggt tgattgattg
    12481 tttcccacac gcgcaatggg caaaagagat tgatgtcagt gataaagagg cgcgctgcgg
    12541 tgtgcgttgt gccactcgcg atcatctgcc aatggtaggc aacgtgcctg attatgacgc
    12601 tacactcgtg gaatatgcgt cgctggcgga gaagaaagat gaagcggtaa gcgcgccggt
    12661 ttatgacgat ctctttatgt ttgctgcttt aggttcgcgc ggtttgtgtt ctgccccgct
    12721 gtgtgccgag attctggcgg cacagatgag tgaagaaccg attccgatgg atgccagtac
    12781 gctggcggcg ttaaacccga accggttatg gatgcggaag ttgttgaagg gtaaagcggt
    12841 taaggcgggg taatctgctt tggcattgtt tgtcggatgc ggcgtaaacg ccttatccga
    12901 cctacgcgtt acctgtaggc ctgataagac gcgataagcg tcgcatcagg catggtgctc
    12961 cagacgtcac aacttactgc tgtgaggcct gctgaaacag gtgttcccac atatcggtta
    13021 ccagcgcctg gtcacgcggt gacaattcac ccgcgccaat ggctttttcc agactctggc
    13081 taacggttgt atgtaccgcc tgagcggagt ggtcatcacc actttccagt tctgcgatgg
    13141 ctaatgtcag gtggccacgc aaatacccac tggcaaacaa ctcatcatca ctggcatggt
    13201 caaccatacc gtcgattaat gccagaatgc gtgattcaaa ctccgcgatc atcttctttc
    13261 ctcattgtaa aacgtggcgc tggcttcagt tgagcgcttc cggccacggg aactgttccg
    13321 ccgtaagttc cggcgtgtga taataatttt gtagtgcttt aatgaagcgt gccggacgtt
    13381 cgggaatgcc gtttgccaga taatccatca cctgcgcatg tacccgccgt tgaaacacca
    13441 cgcggtccgg ttcgaaatcc ccttccagat tgtcgcagct aacattaaac ggatatcccg
    13501 ccgccacgca gaacaaccag tccagcgcct gcggcttcac ttcaacatct tcaaactgac
    13561 tttgcgtctg ggcatcgcgt ccgtccgggc aataccagta accgaaatca accagctcac
    13621 ggcgcgcttt cccggcaata caccagtgcg aaatctcgtg gatggcgctg gcataaaagc
    13681 catgagcgaa gacgattctg ttatacggta cttccgcatc agcaggaaga tagatcggtt
    13741 cgtcgtcgcc tttaatcaga cgggtattaa attcatcggc aaagcagcta ttaaaaatct
    13801 caatcaactg ctcatagtgg tgtgtactgt tcattagttc atccccaacc agtggaggat
    13861 ctcctgtcca tggctatcgt aaagtaattt ggcactcatc accgccgaga cgataacgat
    13921 catcgggcgg atcagctttt gtcctttgct caacaccagt cttgaaccca tgcgcgcgcc
    13981 gaggaactgc cccaccagca tcacaaagcc cgtcgcccaa atcactttgc cgccgagaat
    14041 aaacagcagc agaccgccaa tgtttgacgt tgcgttaagt aatttggcgt gagcagtggc
    14101 tttggcgagg ttgaatccgc acagcgtaac gaaggccagc gcgtaaaacg atccggcagc
    14161 cgggccaaag aatccatcgt aaaaaccgac gcagcccccc gccaccagcg caaatggcag
    14221 tccatacatc cgacgctggc ggtcttcttc acccagcttt ggcatcagca agaaataaag
    14281 accgatacaa atcaccagaa taggcaaaat ctgccgtaag acatcggcct gaacatactg
    14341 caccagtagc gcgccgctca ttgagccgac aaaggtcatg gcgatattga gtttttgatc
    14401 gcttaaacta accactttac ggcgaataaa gtagatagtg gcggaaatag agccaccgca
    14461 ggcttgcagt ttattggttg ccagcgcgtt agcgggagac atccccgccg ccatcaatgc
    14521 cggaatggtg agtaacccac cgccaccggc aatcgagtcg ataaaccccg ccagcatggc
    14581 gacaaaaaag agaactccca gcagcagcgg ggaaaccata aacaggctat taaacgtttc
    14641 cattagatca cgtgctcatc cagtagcgcc tggcaggaag gcggcaacgg aggcggtgtc
    14701 ttcttctcag gctttgttgt tcccggtttt ggaggttcaa accagctttg cagttctgcc
    14761 ccgcaaccat cgcctggtgg cggtaaaggt tgatcctcac actccagact atcggcagga
    14821 caacgtaatc gtacatgcat atgcgcgcga tgctggaacc agggtcgcac tttgcgcaac
    14881 cagtcgcgat cggtgcccgc atcaaggcaa agttgttgtt taatcgccgg attaacaaaa
    14941 atgcgcgtga cgtctttgtc ctgggcggcg agtttgatca agctgaaaat ttctggcttc
    15001 cacagcgcgg ggacaacgtg tttaccgtcg cgggaaacta aatctaacgc ttgcgggcgc
    15061 aagagctgcg cggaggtcca gcgtgttttc ggcagttgca ggaagatatc gacatccagt
    15121 ccggtctggt ggctggcgtg accgccgttg aaacgaccac cagcaggcat ccccatatcg
    15181 ccaatcagca ccgtacccat gcccagattg ctcacctggc gactcagacg ctggataaac
    15241 accaccagat ccggatgtcc gaaatagcga cgctgatcgg tacgcatgac ctgataatgt
    15301 tcggactgta tcggcaacgt gtcagcaccg acgatacagc cattagaaaa actgcctatc
    15361 gattgtgcgc tacccggcac aggttgggtt attttttgcc acggcgtcgc tgccaggctg
    15421 gcgctactgg caagcagggc cagcagcgca atcgcagttt tgttcatttt ttaccagcgt
    15481 ggaatatcag tcttcacatc ggcattttgc gcccgctgcc gtagcaggtg atccatcaaa
    15541 acaatcgcca gcatcgcttc tgcgatcggc accgcgcgga tcccaacaca aggatcgtga
    15601 cggcctttgg tgatcatctc aacttcttcg ccaaagcggt taatagtgcg ccccggcacg
    15661 gtaatgctgg aggttggctt cagcgccata tgggcaatga tttgctgccc gctgctgata
    15721 ccaccaagaa tgccgcccgc atggttgctc tggaaaccgt ctttggtgat ttcgtcgcgg
    15781 ttctggctgc cacgcagcgc taccacgtca aaaccatcac caatttccac gcctttcacc
    15841 gcgttaatgc tcatcagcgc atgggcgatg tcggcatcca ggcggtcaaa gaccggctcg
    15901 ccaagtccgg cgggtacgcc actggcaacg acggtgactt tcgcaccaat agagtcgcct
    15961 tcttttttca gcgcgcgcat cagttcatct aacgcgtcga ttttgtccgg gtccgggcag
    16021 aagaatggat tttgctcgac ctgagaccag tctttgattt ccagtggaat gtcgcccatc
    16081 tgggtcaggc aaccgcgaat ctcaatgcca aatttctcgg cgagatattt tttggcaatc
    16141 gcccctgccg ccacgcgcat agcggtttca cgggcggaag aacgtccacc gccgcgataa
    16201 tcacgcaggc cgtatttttg ttcataggtg taatcggcat gacctggacg gaaaacgtcc
    16261 ttaatcgcac tataatcctg agaacgctgg tcggtatttt cgatcaacaa tccgatgctg
    16321 gcgccagtag taacaccttc aaaaacaccg gagagaattt tgacttgatc cggctcgcgg
    16381 cgctgggtgg tatagcgcga tgtcccaggg cgacgacgat caaggtcatg ttgcaggtcc
    16441 gcttccgtca gcggaatgcc tggcggaaca ccatcgacga tgcagccgag cgccagcccg
    16501 tgcgattcgc cgaaggtggt tacgcgaaag agttgtccaa ttgtgtttcc agccatcacg
    16561 gctccgttat tgttgtgttt gcgtgtttac ttaatcttta taaatcgcga aatgttcacg
    16621 ggccgccagc agctgctctt tggtgagcat aaacacacca tcgccgccgt tatcaaactc
    16681 cagccatgtg aacggaacat ccggatattg ttccataaga tgtaccatgc tgttgcccac
    16741 ttcacaaatc agcacgccat catcagcgag gtaatctgcc gcgttaccga gaatgcgacg
    16801 cgtcagtttc aggccgtcgg tgccagatgc cagacccagt tccggctcgt ggcggtattc
    16861 gtttggcagg tcggacatat cttctgcatc gacgtacggc gggttggtga cgatcaggtc
    16921 gtactgcact ttcggcaagt cacggaacaa gtcggaacga atcggaatga cgttgtggat
    16981 caaaccgtgt tcttcgatgt tctgttcagc aaccgccagc gcgtctggag agatgtccac
    17041 cgcgtcgact tccgcttccg ggaaggcata agcacaggca atggcgatgc agccgctacc
    17101 ggtacacata tctaaaatat gctgcggttg cttgctgatg agtccggcaa atttattgtt
    17161 gatcagttca ccaatcggcg agcgcggcac cagcacgcgt tcatcgacgt aaaattcatg
    17221 gccgcagaac caggctttgt tggtcaggta agccaccgga atgcgctcgt tgacgcggcg
    17281 gatcacgcgc tcaacaatac ggtgtttttc gctggaggtc agacgcgcgg tgcgcatgtc
    17341 ttccggaata tccagcggca ggtagagcga aggcaacacc agttgcacgg cttcatccca
    17401 cgggttatcg gtgccgtggc cgtaccagat attcgccgcg ctgaagcggc tcaccgacca
    17461 gcgcaacatg tcctgaatgg tttgcagctc atttactgct tcatcaacga aaattttatc
    17521 cacgtattcc tccagggcat gatcgcaata atttcggcgg ctagtttgcc atgaagatga
    17581 cgataaatca gcattcacgc gcggtgagtg aggaaaaata cgtttaaaac gatcgattgt
    17641 gctacgagtc gggtaaactg taggaaaatt agaaatagag aaagataaat gaaaaagaaa
    17701 acaacactta gcgaggagga ccaggcgctg tttcgccagt tgatggcggg tactcgcaag
    17761 attaagcagg acacgatagt ccaccgaccg cagcgtaaaa aaatcagcga agtgccagta
    17821 aagcggttga ttcaggaaca ggccgatgcc agccattatt tctctgacga gtttcagcca
    17881 ttattaaata ctgaaggtcc ggtgaaatat gttcgcccgg atgtcagcca ttttgaagcg
    17941 aagaaactgc ggcgcggtga ttattccccg gagttgtttc ttgatttgca cggtctgacg
    18001 cagctacaag ccaaacagga actgggggcg ttgattgccg cctgccgccg tgaacatgtg
    18061 ttttgcgcct gtgtgatgca tggtcatggt aagcatattt tgaagcagca aacgccactg
    18121 tggctggcgc aacatccgca tgtgatggca tttcaccagg caccaaaaga gtatggtggc
    18181 gatgcggcgt tgttggtgct gattgaagtg gaagagtggt tgccgccgga attaccctga
    18241 acattaaaag gtggatgagt cgggccattt agccttgaat actggcgtct cacccctggc
    18301 aataatgaac gtaataatcg ggcattaatt gaaaaacgta agcagctttc cgacagcctt
    18361 ctgtgggcta atggcattgc cccttcagag gggcttgagg atgacgacct gctgcaagcg
    18421 cttatcgacg aattactcac cctcgttgac ggttatctgg cacctttcgc tcctgtggtg
    18481 gcgaatattc taaaaaactg gatgaccgca tgacataacg gacaacggga ccagatggcc
    18541 ccgcatcgtg tgtggtgtat acgctacagc tcgttgagcg tgtactggaa gcggcaggtg
    18601 gaatcaatcg gctggcggct gctggtatcg acacgcacta ccatgtcgtg cagttgcttg
    18661 tcagtgatgg gatgctggtt gggtgtgacc gtcactttcg aactgtcaca gccggaggtg
    18721 gagaaggtga tcatctgcgc cacaaagcgg cagtatgcgc ctcgatcgtc catcacgctg
    18781 ccgtcacaga gcttgccgct ttccatcaaa aaggtgggca gcgttttctc aatcgttcca
    18841 cccgtcgatt gcagcgacag cactttcgaa tggacaacat aatcttcact ccacgaaata
    18901 tcataagcgg cgacggagac ggggcatgat gagttgtgaa ctttggggcc aatatataga
    18961 ccaacaccat ctaccgaaga gatattgctg tcagtatatg tcgagatcaa ccacgggcaa
    19021 ccgtgcacct caccatcata gtacactccg aggctggcgt tgtaacagat ccaatcagat
    19081 ttactcgacc gagcatcata cgcgtcaaga ccaacacagg tagattttga agcatcattg
    19141 agctcctcca ttttcaacgt cggtgcggcg gtactccctg tccaggaaac taaatctaaa
    19201 ttctcagctt gaagcacaat actgtgttgc ttataaacgc catactcaac cccttcaggg
    19261 gtcagaattt caagataata acgtatctgt gttagatacc ccgaatatgc caccggggtc
    19321 accaatgtcg caattttctt cgctgccaga ctttctgctg cgcaagtcag catgaccagg
    19381 cagcacaacc atcggaatat tttcatttta tatgtactca aatgtcgcag tcagggaggc
    19441 ggtaaaatcc cccatcgtca cttcccggcc gcttttcgcc tggagccagg cattaaaatt
    19501 cagattgtta ttgccgttac tcagcacaaa ggtcgcgccg ctggtattgt taatcggcac
    19561 cggtgttcca tcggtttttt ccatgccaat tccgacacct tgcgccgccg aagatgcatc
    19621 cagcgccaga aaaccgggct gttcgctgtc ttccgtgccg gtgagcgtca cccgaacctc
    19681 atactgggct ggccctttgc aatctttcag ctgaaacact accggcaccc gcgccgactg
    19741 cccggtcacc atcaggtctt tacggctgat ggtgggaaaa tgcacctccg ccagcgactc
    19801 gccctgaacg actaacgtgc aggacttgct gagtaaatta ccgtagaaat gcaggttgtc
    19861 agccgcctga acatggggcg tcagcagcac cgccgccagt gcagccgcgc agtgagatat
    19921 ccgttttagg ttcattggta atccacctgt aatgttgcgt aggcagaaaa ggtgccttcg
    19981 ctgagcgccg catcattggc tttcatcggc acagcttgca gcgttggcgg cgttgacggg
    20041 tcaattttca acggtgttcc cagcttaaac ggctggccgt tttgctgtag ctcaatcccc
    20101 aggccgctaa cgtccgtttc aatggcggaa tcatcataag gggtttggct gcccgtccag
    20161 gtcaggctca tctcccaggc gtcgtcgcgc acatcggggt cacatgtgat ggtgtaaggc
    20221 acatcctgac ggaaattatc gccgttaatg ttgtcgataa tgacgttgcg aaactcaact
    20281 tcgatcgtct taccatcgct gatagtacag gcgggtggcg aaaccagcgt gccgtgaaaa
    20341 gtaacgtcgt taatcgcggc ctgcgccagg ccagcaaaac cgtaaattaa gcaccatgcg
    20401 agtcgtttca ttggtagtcc accaccatgg tcatggtcgc gttaaactcc gatgccatca
    20461 gttgcacacc gctctgcttc accggaacgg cttcgaagtc tggctgtgta ttgatgttga
    20521 acggcagcca gctactgtta cccactgcca ccagcgaatg gtcagcggca ttctgaatac
    20581 gaatgccaaa accgctgatt ccagtactca gcaccgtttc gccgttgatg gtcgtggtgg
    20641 tggcctgaag ctgcatttgc aggtcgtcgc tgacactgtt tgagcagttc agggtgtagc
    20701 ccactggctg acggtagttg gcaccatcaa tgcttttaat aatcacattg ccgaactcca
    20761 ccgcgcttcc ctttaccgaa cagggcggcg gtccatccac cagcacgcgc aacgtcaaat
    20821 tgacgatttt gccgcccgcc attaccggtg aaacgatcgc gccacaggcc agcagtagcg
    20881 cggcagatcc ggtcagaaag aacgctttca tgttgcttct cccgttatca acctttgctg
    20941 ctttgatctt catccacctt gcaggtgttc ccttcacaac ggaagatcag cggcatgcgc
    21001 gcgccatagt cgttgatata ggtcagcacc ggggcgctcg ccaattttgc ctttaacggt
    21061 tgtgacgttt tcggtggcat caccagcggt ttaaacccgg cggcagtcag ccctttcttc
    21121 tgtgtgctgg catcgctgat gatgacgtgg tacggcgtcg ggttattgac ggtatagtcc
    21181 tggccactgc gcgtgagtgt gaccttaaac tgccacggtc ttctcatgtc aatcttctcc
    21241 agcgcctttg gtcgccagaa cagcttgatg cgcgtttgca gcgcaatttg cagggtgttg
    21301 gctttcccgg actttggcgg gatctcacgc acgttgtagt agaacaggct ttctcggtcg
    21361 gacggcagcg tgtgaatgtc cggcagcgcc tgaatcttca cctgaccgtt catcatagcg
    21421 tcaatacgct gcaccggcgg cagcaccgcc agcggcgaag tgattttgtt gcccttctcg
    21481 tcttccagcc agctttgcgc caggtaaggc agcttttcgt tgttgttacg caacgtcacg
    21541 ctgatcgatt tatcgctttc gttaaacacc agacgggtgc gatccggggt gaccgatgca
    21601 tgcgccgtca ggctggcggt ggtaagcagt gccatcagcg ctgtgctgac gagcattgga
    21661 caggataatt tataagacat tgatcgttcc ttaatcagat tattgagttg ctgaaattga
    21721 cgtcggcgct tccgttggtg tgacttgccg cgtctggtcc tgaataacag gcttaatttc
    21781 tggcgctgaa gaatcagggg ctgccgttcc cttttgttca cacggcaaca acaggccgct
    21841 gaacaaatcg gcaggtagcg gcttcggcaa tacgatctcg cattgtgcgc tgccttccca
    21901 gaacaccatc atatgctcgc ccgcattgac ccccgccaga tagacattgc cttcatcatc
    21961 cacaatgcca acctgctgct gctcgtcatt tttcacttcc gcgccaaacg gcgggtagct
    22021 accgtcacgc aacctcagta ccgccatcgc tttttgcccg ctgatcactt tgaatttacg
    22081 atagccgatt gcgccttccg tcagagtcgc ctgcaccacc gactgggtgg cttccgcatc
    22141 ttccggcaag ttgtttaaat ctatatacgc ctgattgcga tagtagttgt taatatcggc
    22201 gaccaccgcc ttgccaaaca tattggtgta caccggcgcg ccgttgcttt cgaccggcac
    22261 gttggcaatc ccgtcggcgt caatcagcag gcgcgtgccg cccatgttct gggtacggtg
    22321 cagcgcccca ccatgcgcgg tcagcgttgc cccgccctgt aaggcgatcc ccgccgaacg
    22381 gtattcccct tcatggtagt tggcgctgag atcaaccttc gccagcgaac cgtcgtggct
    22441 cagataacca tccacgctgc cgtgctgctc gctggtgcca acgttaacct ggtagtgcgt
    22501 tgcgtcatcg acgcgcttaa agtaaccgac ctggctgctg tccgacccgc tgccgtagga
    22561 accgttgtag gtcacggtgc tgctgtcgct ccagggaatg ctcatactga ggtacatccc
    22621 cttatccgca ttatcgtcat attcgtagcg atagccggtc accgagatgc tcatgttgcg
    22681 aatgctcccc atattaaaat agtgggaaaa catcaagtta tagtttgtct gttccgggcg
    22741 atcccagtaa gtacgatggg agtagttgag atagatcgaa acacccgcag cggcaaagtt
    22801 ctggttatag gtgatcgtat acatctcttt gtcgttaccg gtccgcgcca tgtccgactg
    22861 gttcgcgtcc agatactcgc tcatggtcat gaagttcttt tcagaaaaac ggtagcctgc
    22921 aaaggtgacg cggctgttga gttcgtcaaa gtctttggca tagctcacgc gaaaggagtt
    22981 gccgtccagt tttcctttgc cgtatgcgct gtcatgatcc aggttgacgt gcgagtgagt
    23041 cacatcgaac gccagcgcgc caaactgtgc gaggtcgcgc cccaccccca tcgccgccga
    23101 ctgatagtgt ttatctgcca gcgcaccacc gtagagcgac cagccatctg ccacccccca
    23161 tgacgcttct ccgccggaga aaaagccgcc ttcggtcttg tggttccagt cttccggacg
    23221 ccccatcatc actttgtaac gcacctgccc ctggcgcgtg aggaatggca tagatgcggt
    23281 cgtaacgtca tattcctgca cctgaccatt ctgttcttca acgcggacgt gcagtgtgcc
    23341 ggagacggag tcgccgatat cctgaatgcg gaatggcccg gctggaacct gggtttcgta
    23401 aagtacccgg cccatctggc taatggtgac ttttgcactg ctgtgcgcca cgccggaaac
    23461 atccggtgca tagccgcgca ggttgggcgg cagcatttgg tcatcggtgt tgacgctgct
    23521 gccgatatag ttaaagccgt cgaaaatatc ggaattgaga taatcttccc ccagcgacag
    23581 cttcgctttt aaagagggca aggctcgcca ggcataataa cgactccagt cccagtgttt
    23641 actcgttgtg ctgttactgc tgtcatcgtc gtcatcgttg ctgcgggtgt gctgataatc
    23701 actttgccag tctgcacgga aacgccatgc ccccaggttc gccccaacgg taccgttgcc
    23761 gctgatatca tgtgagtcct cgcccccctg ctcctggtgg cgagtttgcg cattgaggct
    23821 gtagtcggca atcaggccag gaataccgtc gtcccaacgt gagggagggt cccagtcgct
    23881 gctggtatat tcgaggtaag cctgtggcac tgtcagcagc aacgccgact ggcttaaatc
    23941 attctcgact tccatcccgt ctaattgacc tggtttaagg cattcatcgt tgtgcgtcca
    24001 ctgcaggctt tttgctatcc cttctttcag ccccagcgcc gccactaact caggtgtcag
    24061 gcaggcatag ttttttgttg gatcgttttc agagacgtac cagttaatgt cgtattcatc
    24121 agaaaggggt tgtttattta taaatacacg taaattatat ttacccgcgt cgacataccc
    24181 tttttttgaa aacttaccga gatcaatttt cgtatcgccc tttaactcaa ggaaacgggg
    24241 gtcaaattga attgaatcat cagcccatgc attgactgac ccaccagaga ttgccagcgc
    24301 aatatagcag gcgattcccc gcagccgaaa atttgagtga ttaggcatac acatatccgt
    24361 ttgtgaaaca gtcgtaaatc tgttgcgtgt tgaataaccc cacagcagaa cgctgtgggg
    24421 ttgtcacatc agagataggt aatctggaat gtggtcaggg tttcgaactg gccgagatcc
    24481 ggcgcatcgg tttcacccac cagccaggct ttaaagttca gcgtttgctt gtctttaccc
    24541 ttgctggttg aagtatccat cacgattggc tgaacaatct tttagcccac tttataacct
    24601 ttgccatggg tatcgccgat taccaggctc acgtggttca tggcaacacc gctgtcaaca
    24661 ttgaataacg catagttatc cgtgttggct ggccccgcgt agttagtgcc ggtgaacgtg
    24721 gtctccatgt ttgtttcagt ggtaaacacg caatcctgta aacggatctg gaaatctttc
    24781 ggtgtggtag tgccaccgcc ggtcagttta gccgcaccga tgtcacccat atcgacttcc
    24841 agtttgtgat cgtctggaac gatagagcac ggggagtctt caatggtgcc gtagaagcgc
    24901 gcggtaccgt tgttgcctgc tgcaaaagca gaactggaaa caactgcgcc catgaccatc
    24961 gccgcagcaa tggctgtttt ggcaaactta ctcattattt gtccttaaat cattaagagt
    25021 tataatataa gcacctgaca taaaattaat atatattatg cccgatattt atagttagcc
    25081 att
//
LOCUS       DADTFG010000066        24934 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.066, whole genome shotgun sequence.
ACCESSION   DADTFG010000066 DADTFG010000000
VERSION     DADTFG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.066"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            51..326
                     /locus_tag="J1138_002470"
     CDS             51..326
                     /locus_tag="J1138_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA8454252.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
     gene            complement(403..645)
                     /locus_tag="J1138_002471"
     CDS             complement(403..645)
                     /locus_tag="J1138_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBA8454253.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            998..1918
                     /gene="lpxP"
                     /locus_tag="J1138_002472"
     CDS             998..1918
                     /gene="lpxP"
                     /locus_tag="J1138_002472"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBA8454254.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2274..2345
                     /gene="ypdK"
                     /locus_tag="J1138_002473"
     CDS             2274..2345
                     /gene="ypdK"
                     /locus_tag="J1138_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBA8454255.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2410..3648)
                     /gene="alaC"
                     /locus_tag="J1138_002474"
     CDS             complement(2410..3648)
                     /gene="alaC"
                     /locus_tag="J1138_002474"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBA8454256.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDMAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4025..5722
                     /locus_tag="J1138_002475"
     CDS             4025..5722
                     /locus_tag="J1138_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA8454257.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVMAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5737..6474
                     /locus_tag="J1138_002476"
     CDS             5737..6474
                     /locus_tag="J1138_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA8454258.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTTPAATVTRENDTINLVKDERIIVTPINDIYYAE
                     AHEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTY
                     ILRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6487..7344
                     /locus_tag="J1138_002477"
     CDS             6487..7344
                     /locus_tag="J1138_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8454259.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQHMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARIILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7347..9842)
                     /gene="ptsP"
                     /locus_tag="J1138_002478"
     CDS             complement(7347..9842)
                     /gene="ptsP"
                     /locus_tag="J1138_002478"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA8454260.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     ICAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDKKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGELQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9867..10904)
                     /gene="ypdE"
                     /locus_tag="J1138_002479"
     CDS             complement(9867..10904)
                     /gene="ypdE"
                     /locus_tag="J1138_002479"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBA8454261.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLDEADRQQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAAHQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10904..11989)
                     /gene="ypdF"
                     /locus_tag="J1138_002480"
     CDS             complement(10904..11989)
                     /gene="ypdF"
                     /locus_tag="J1138_002480"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBA8454262.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRRQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYFPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(12005..13252)
                     /locus_tag="J1138_002481"
     CDS             complement(12005..13252)
                     /locus_tag="J1138_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBA8454263.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAVGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIESL"
     gene            complement(13274..13600)
                     /locus_tag="J1138_002482"
     CDS             complement(13274..13600)
                     /locus_tag="J1138_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBA8454264.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13819..14784)
                     /gene="glk"
                     /locus_tag="J1138_002483"
     CDS             complement(13819..14784)
                     /gene="glk"
                     /locus_tag="J1138_002483"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBA8454265.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14988..16244
                     /locus_tag="J1138_002484"
     CDS             14988..16244
                     /locus_tag="J1138_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBA8454266.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPIPPSALPG
                     LIVALILGLAGGVSLGPEHPIITVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRQKQQPPHDNV"
     gene            16359..16684
                     /locus_tag="J1138_002485"
                     /pseudo
     CDS             16359..16684
                     /locus_tag="J1138_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
     gene            complement(16825..18063)
                     /gene="mntH"
                     /locus_tag="J1138_002486"
     CDS             complement(16825..18063)
                     /gene="mntH"
                     /locus_tag="J1138_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HBA8454267.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18399..19601
                     /gene="nupC"
                     /locus_tag="J1138_002487"
     CDS             18399..19601
                     /gene="nupC"
                     /locus_tag="J1138_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBA8454268.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19651..21840)
                     /locus_tag="J1138_002488"
     CDS             complement(19651..21840)
                     /locus_tag="J1138_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HBA8454269.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLSFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKIRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLIMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETPQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22046..22121)
                     /locus_tag="J1138_002489"
     tRNA            complement(22046..22121)
                     /locus_tag="J1138_002489"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22086..22088),aa:Ala,seq:ggc)
     gene            22357..22701
                     /locus_tag="J1138_002490"
     CDS             22357..22701
                     /locus_tag="J1138_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBA8454270.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            22703..23095
                     /locus_tag="J1138_002491"
     CDS             22703..23095
                     /locus_tag="J1138_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBA8454271.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23146..24561)
                     /gene="gltX"
                     /locus_tag="J1138_002492"
     CDS             complement(23146..24561)
                     /gene="gltX"
                     /locus_tag="J1138_002492"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBA8454272.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLNLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            24820..24895
                     /locus_tag="J1138_002493"
     tRNA            24820..24895
                     /locus_tag="J1138_002493"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24853..24855,aa:Val,seq:tac)
BASE COUNT         6257 a         6575 c         6090 g         6012 t
ORIGIN      
        1 acagccataa catcgtaggt tttcagtgaa ccattttcag gatattttct atgaaagtaa
       61 acttaatgct ttttagctta tttttatttg tctctattat ggcatgcaat gtttttgcat
      121 tttctatttc tggtggtggt agtgaggcga gctataaaga gacagaaaaa acgtcagtga
      181 tgacgacaac gcattctaca aaacttcagc catcacaggc gattttgttt aagatgagag
      241 aagatgcacc accattaaac ctcacagaag acatcacgcc tacttatccg acaaaggcga
      301 attatctcat tcatcctttg cgatagcgtc tgtcacctgg caggtgaaca atcatgtctg
      361 atgctgatgt gaacacctgc ccaaatgagc catcctgacc cactatcgcc tgccataccc
      421 caccataaag cgaatcatca agccacaagc cggggcaaga aacataaata tccccgtcac
      481 cagttcatta ccgccagtta accacatggt gaagtaataa atgagtgcag cgaggatcac
      541 catgccgaag aaagaagaga tcgccgatac catgttcagt acctgaccga tgtatccgct
      601 tacgcagaca atacacaggg cgagaaacat ccataaataa atcatagctc aacctcctgc
      661 cgcgctaaaa aacctgtaac ttcagtcact ttaatagtaa gacaatcctt aacctctggc
      721 aatttttgct aagcatcaat gaaaacagca tgttaaatgc aagactgttg tgtacggaaa
      781 aatatttact ttgcacgatt aataatcagt agctgaaagc agtcagcgag tgataaagta
      841 cagataccgc attcatgctc ctcgtggtta tgtcctgact agtctttaca ctctttacag
      901 gaaccattgt cgtacatgat ggcctaatca attgagtgta ccgctgtcga catggaatct
      961 tctgcgatac aacaattcgt atctacagaa ggtaactatg tttccaaaat gcaaattttc
     1021 ccgcgagttt ctgcatcctc gctactggct cacgtggttt gggcttggtg tactctggct
     1081 ttgggtacag cttccttatc ctgttctctg ctttctcggc acgcgtatcg gcacaatggc
     1141 gcgaccattc ctgaaacgtc gtgaatctat cgcccgtaaa aacctggaac tttgtttccc
     1201 gcagcattct gcgaaagagc gcgagaagat gattgccgaa aactttcgtt cactcggcat
     1261 ggcgctggta gaaaccggca tggcgtggtt ctggcccgac agtcgcgtac gtaaatggtt
     1321 tgatgtggaa gggctggata accttaaacg cgcacaaatg caaaatcgcg gcgtaatggt
     1381 tgtcggcgtc cattttatgt cgctggaact gggcggtcgg gtgatgggac tttgccaacc
     1441 aatgatggcc acctatcgcc cgcataataa tcagctgatg gaatgggtgc agacccgtgg
     1501 tcgcatgcgc tctaacaaag cgatgatcgg cagaaataat ctgcgcggca ttgtcagtgc
     1561 actgaagaaa ggtgaagcgg tatggtttgc tcccgatcag gattatggtc gtaaaggcag
     1621 ctccttcgcg ccgttctttg cggtggaaaa tgtcgccaca accaatggca cctatgtcct
     1681 ctctcgtctc tctggcgcag ccatgttgac cgtaacgatg gtaagaaaag cggattacag
     1741 cggatatcgt ttgtacatca ccccagagat ggaaggctac ccgacagatg aaaatcaagc
     1801 cgctgcctat atgaacaaga ttatcgagaa agagatcatg cgcgcaccgg agcagtacct
     1861 ctggatccac cgtcgcttta aaacgcgccc ggtgggtgaa tcgtcgttat acatttaaca
     1921 aacggctctc actctttccc tcctcaaatc agccataata acctcatggt ttttatggct
     1981 tttttcttaa ggttttttcc ttcgctgaat atctaattat taccgtcaga aattaaactg
     2041 tcgccgatat gagacacctg taagttacta aaagtaaata agaaaatgcc tcttgtctcg
     2101 tttcagtttt aggcgtacat tagcgccgtc tcgagcagtt tagctcagaa ttctgggatg
     2161 atgcccacgg taaaatacag gacgtttaag gtcgttttgc ccggtgtcaa ctcactgtat
     2221 caggtgtaat aaagtcattc aggcgtaaca gtagttacgc ggagagatgt aaagtgaaat
     2281 atttctttat gggcatttct tttatggtca tcgtttgggc cggtactttt gccctgatga
     2341 tctaaaagca aactgtgcaa tgaaaaacag gagccatctg gctcctgttt gcatttctga
     2401 cgatggtgtt tattccgcgt tttcgtgaat atgtttgctg ctggcaggta gtaaaccgtc
     2461 ggcacggaac atcgctttaa tcccacgaat cgcctgacga atacggtcgc ggttttcaat
     2521 cagtgcaaag cgcacatgcg tatcgccgta gtcgccaaag ccaatccctg gagagacaca
     2581 gaccttcgct tcgttaagca atttcttggc aaattccagc gagcccatgg cagcatacgg
     2641 ttccgggatt ttcgcccaga catacatcga agccttcggc atttcgacca tccagcccgc
     2701 ttcatgcagc cctttcacca gcacatcacg gcggcgtttg tactgttcgg caatatcgcg
     2761 cacgcactgt tgatcgccct ccagcgccgc aatagccgcc acctgcaacg gcgtaaaggt
     2821 gccgtaatcg tgatagcttt taatacgtgc caacgcgctg accagcgttt tgttaccgac
     2881 cataaagccg atacgccagc ccgccatgtt gtagcttttc gacagcgtaa agaactcgac
     2941 cgccatgtcg cgtgcacctg gcacctgcat gattgacggc gctttccagc catcgtagac
     3001 gatatcggca taggccaggt cgtggaccac cagcacatcg tagcgtttcg ccagcgccac
     3061 aaccttctca aagaattcca gttccacgca ttgcgcggtt gggtttgacg ggaagccgag
     3121 gatcatcatc ttcggtttcg gataactttc acgaatggcg cgctccagtt cgttgaagaa
     3181 atcgacacct tccaccagcg gcactgagcg tacctgcgcc ccggcaatca ccgcgccgta
     3241 aatatgaatg gggtagcttg gattcggcac cagcactgtg tcaccatgat ccagcgtcgc
     3301 cagcatcaga tgcgccaggc cctctttcga accaatagtg acgatggctt ctgattccgg
     3361 gtcgatttca acgtcgtagc gatcctgata ccagcgggaa atggcgcgac gtaaccgcgg
     3421 aatgccgcgt gaagtggagt aaccatgcgt gtccgggcgc tgggccaccg tacataattt
     3481 ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc atactgaaat cgataatatc
     3541 ttcgccgcgc cgacgcgcag ccattttcag ttcagcggta atgttaaaaa cgtagggcgg
     3601 gagacgatca atgcgcgtaa agcgacgttc agggcgagtg tcagccataa tttcctcaga
     3661 ttaacgttag cgcccggacc gtccgagcga cgctgccacg atggtggctg ttttgaaaat
     3721 agcctgatta atttctgtct gtccagcgtt ttagaacatt tttatcaatg gctttaaatg
     3781 aggaactcca cttcaaaaac aggaaattta tctacgctaa caagaaaatt accactcatt
     3841 ttattaacat tataatttca ataaatttac caaacggatg tacattatca agagatgcat
     3901 tgctaataca ccatttatct ttcaatcccc ttttaaaatt gtggtttttc ctcaaaaaag
     3961 gcctgctggt catcagcctt caggttacct atcatagagg tttaatcctt attcagagtc
     4021 acccgtgcac gaaatattca acatgctgct ggcggtcttc gatcgggcag cgttaatgct
     4081 tatctgcctg ttctttctca tccgtatccg cctgtttcgc gaactgttgc acaagtcggc
     4141 gcactcaccg aaagagctgc tcgccgttac cgccattttc tcgctgttcg ccctgttcag
     4201 cacctggtcc ggcgttcccg tagaaggctc gctggtgaac gtacgtatta tcgcggtgat
     4261 gtcaggcgga attctgtttg gcccgtgggt gggcatcatt accggcgtga ttgcgggtat
     4321 tcaccgttat ttaattgaca tcggcggcgt aatggcgatc ccctgcttta tcaccagcat
     4381 tctggcgggt tgtatatcgg gctggatcaa cctgaaaatc cccaaagcac agcgctggcg
     4441 cgtcggtatt ctcggcggca tgttgtgtga aacgttgacc atgattctgg tgattgtctg
     4501 ggcaccaact accgcattgg ggatcgatat cgtctctaaa atcgggattc caatgatcct
     4561 cggcagcgtc tgtatcggct ttattgtgtt gctggtacaa agtgttgagg gagaaaaaga
     4621 ggccagcgcc gcgcggcagg ccaagctggc gctggatatc gccaacaaaa cgctgccgct
     4681 gtttcgccat gtcaatagcg agtcattacg caaagtctgc gaaattatcc gcgatgatat
     4741 tcacgccgat gcggtggcga ttactaatac cgatcatgtg ctggcctatg tcggcgtggg
     4801 cgaacacaac tatcagaatg gcgatgactt cattagcccg actacccgac aggcgatgaa
     4861 ttacggaaaa atcatcatta aaaacaatga tgaagcccac cgcacaccag agattcattc
     4921 catgctggtg atcccattgt gggagaaagg cgttgtgacc ggaacgctga aaatttacta
     4981 ctgccacgcg catcagatta cgtcatcatt acaggaaatg gcggtcggtc tgtcgcaaat
     5041 catctccacg caacttgagg tgtcacgcgc cgagcagtta cgtgaaatgg caaataaggc
     5101 agagcttcgc gccctgcaaa gcaaaattaa tccccatttt ctgtttaacg ctctgaacgc
     5161 tatttcatcg tcaatccgtc tgaatccgga tactgctcgc cagctgatct ttaatctgtc
     5221 gcgttatctg cgctataaca ttgaattaaa agacgatgag caaatcgata tcaaaaaaga
     5281 gctgtatcaa attaaagact atattgccat tgagcaggcc cgctttggcg acaagctgac
     5341 ggttatctat gatattgatg aagaggtgaa ttgctgcatt cccagcctgc tgatccagcc
     5401 gttggtggag aatgccattg tccacggtat tcagccttgc aaaggtaaag gcgttgtcac
     5461 catcagcgtt gcagagtgcg gtaatcgggt acgcattgcg gtgcgggata ccgggcacgg
     5521 catcgatcca aaagtcattg agcgggtcga agcgaacgaa atgccgggca ataaaattgg
     5581 cctgctgaat gtccatcatc gcgtgaagtt attgtatggc gaggggctgc atatccgccg
     5641 cctggaacca ggaacggaaa ttgcgtttta cattcctaac caacgcaccc cagtcgcctc
     5701 acaggctacg ttattgcttt gagccggagt gatattgtga aagtcatcat tgttgaagac
     5761 gaattcctgg cacaacagga actgagctgg ctaattaaag agcacagcca gatggagatt
     5821 gtcggcacct ttgacgacgg tctggacgtg ttgaagtttt tgcagcataa ccgcgtcgac
     5881 gccatttttc tggatatcaa tattccgtcg ctggatggcg tgttgctggc gcaaaacatc
     5941 agccagttcg cccataaacc gtttattgtg ttcatcaccg cgtggaaaga acatgcggtg
     6001 gaagcatttg aactggaagc gtttgactac attctcaaac cgtatcagga atcacgcatt
     6061 accgggatgc tgcaaaaact ggaagcagcc tggcaacaac agcagaccag cagtacgacg
     6121 cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga tgagcgaatc
     6181 atcgtcacgc caattaacga tatctattac gccgaagcgc acgagaaaat gacctttgtc
     6241 tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgagttttg cagcaagctg
     6301 ccgccgtcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa caaaatacgc
     6361 gaaatcgaac cgtggtttaa taacacctac attctgcggc tgaaagatct ggattttgaa
     6421 gtgccggtca gccgtagcaa agttaaagaa ttccgccagt taatgcatct gtaatcggga
     6481 actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga tctgttcagc
     6541 ggcctggtgc ttaacccgca actccttggg cgcgtctggt ttgccagcca gcccgcctca
     6601 ttgccagtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct acgcggcgaa
     6661 tacggcaatc tgctggaagc aaagcagcaa catatggtgg aaggagaaat gctgtttatt
     6721 ccggcacgcg cggctaattt accggtcaac aacaaaccgg tgatgctgtt aagcctggtg
     6781 ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc gttgttacat
     6841 cctgctcgcc agatccagct tcccagcctg caacgcggtg aaggtgaagc gatgcttacc
     6901 gccctcaccc atcttagccg ttcgccactg gagcagaata tcattcagcc gctggtgtta
     6961 agtttgctgc atctttgccg taacgtggtg aatatgccgc cgggcaattc gcaaccgcgc
     7021 ggcgattttc tctatcacag catttgtaac tggattcagg ataattatgc ccagccgctc
     7081 acccgcgaga gcgtggcgca gttttttaat atcacgccca atcatctgtc aaaactgttt
     7141 gctcagcatg gaacaatgcg ttttatcgag tatgttcgct gggtacgaat ggcaaaagcg
     7201 aggataattt tgcaaaaata tcatctgtcg attcatgaag tggcgcagcg ttgcgggttt
     7261 ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttggtctgac gccgggagag
     7321 tatagcgcac gttttcaggg ctaacgtcag aaggttaatt ccgtttccag cagcgtcaga
     7381 atactttgtg catcttgcgc ggcaaacagc gactggcgga agtttttatt caccagttta
     7441 cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcgcc cagcgtcagc
     7501 atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt caccaaccgg
     7561 gcaatgctga tactggaatg acggatccac tgtgatttgg tgtgcgggat cgccacgcca
     7621 aaaccaacgc cagtagtaac aatctcttcc cgctgccaga catcttcttc cagctcgaac
     7681 ggatgttcag tacgcccgtt aacaccgagg ttaccgcaca ggaactggat cgcctgctct
     7741 ttattactaa aggactgatc aacaaagata ttctctagtg ccagcagtgg gcgaacgtct
     7801 tcttccggcg caaacgcggt aagtaacgct tcaatttcct gcgcgctgcg gcactcgcac
     7861 gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaagctg gcttttcact
     7921 gccggaatac gcgggctact catgcttagc tcatccaggc ccagccctag cagtagcggc
     7981 agataacgac tttcaccgcc cagttcaccg caaataccta cccatttgcc gcgctgatgc
     8041 gcagcggtaa caatctgctg caacatgcgc aggaacgatg gcgtaatcgg gttatacagc
     8101 ggcgatacgc gcgggttatt acgatcgact gcatacagat actgggtcat atcgttagaa
     8161 ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca caccgacggc
     8221 acttccacca tgatacccag cgtaatcgtc tctgcatgac gcaggccatc gcgcttaagc
     8281 tcaacgatcg ctttttggag ctcacccttc acccataaga tctgatcgag actgtggacc
     8341 attgggatca tcaactgggc gttgccgaaa ctggcagcgc gcaaaatcgc ccgcaattga
     8401 gtacggaaca ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc gaggaacggg
     8461 ttctcttcct ggggaatgtt gaggtaagga atgcttttat cgccaccgat gtccatcgtg
     8521 cggaagataa tcggcttgtc gcccgccgcc agtagcacct gctggtacgc ttcaaactgt
     8581 tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg aaacagccca
     8641 acgccttccg ctccgttggc aaacgcgcct ggggcttcca gagctgtgcc aatattcgcc
     8701 gcaatatcaa tacgcttttt atcacgggaa taggccagct gcgcggcagc ttgcgcctgt
     8761 tgtttttggc gtttatccgc cagcgtctgc gccacctggt aataaccgct caccgcatcg
     8821 ttgggattaa ttgccagtac gccgcactgg gcatcaagca ctgcaggttg cccggcataa
     8881 cgggcaatcg catccagcgg caggccgctc agtaccggga tcgccgaagc gcgggccaga
     8941 atcagggtat gcgaggtgcg cccggttttc tccagaatca tgcccgcaag attttttaaa
     9001 tcgaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt cggtttttca
     9061 agcaccagat tattgcgcgg ttttagttcc ggccaggtga tatgcagcaa ctgttcgctg
     9121 atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc agaaagtttg
     9181 gcacaaatct gctccatatt gctgatgatc gccgccccca gcccctgatg ctgttctgcc
     9241 atcaggtgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa atgggcgctg
     9301 aggatagttt tgctttcgcc gtcacgctca cgcaattgct ggttcagttg ctcggcaagc
     9361 gttgccagac tgtgctccag tagggtggaa tcttgcgcac tcgcagggat cacccgataa
     9421 ctgtcgaggc tgtcgctctg taacagggtc agtgtaccca cgccaacgcc gcttgccagc
     9481 acgttgccgt acagtaaatc cgggttcagg cggcttaatg aacgcggcag cggatgcgcc
     9541 gtcagttctg ccagcgtcgg ctggacgcta tcgctgtcga taaagcgcac ctggatatac
     9601 tcttccagca ctcgccgcgc ctgctcttca tcgctaccgc taatgttcag gctgcaactg
     9661 tcattaaata aagtgccagt gccaatcagc gccagcgagc ttttcgcatc ggcctttgcg
     9721 ttctggcgat ggttaataaa agtgatttcg ctttgccact ggctgcactg ctctttaagt
     9781 tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg aatcgttaac
     9841 ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac aaccgtctca
     9901 cgcgtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat gtcacggcag
     9961 tcggcaatcg atgcggcgca atgtccgtgg cgggttgcag gccccatcac cactgtgggt
    10021 acgccagtgc cggttaagtg caccgcccca ccgtccgtgc cgccattgct gaacatatct
    10081 gactgcaacg gtacgccaat ttctgccgcc acggtttcga tccaggcggt aagttttggc
    10141 ggcgcaatca gtgacttgtc gcttaacacc agcatcggcc cgttaccaat ctggcgatgg
    10201 ttagctgcgc cataatcaaa gtttttcgcc cagcaggcag tatcaagcac aatggcgaca
    10261 tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta atcccacctc ttcgctggaa
    10321 cttgccacca gccacacttc cgcaggcagt tcggcgtcgt gcagctcacg cagtaacgtc
    10381 accagcagat agcaaccgag gcggtcatca aaggctttcc ccatcactcg ctggtgaggg
    10441 agaacctgaa aagtggtatc gaaggtgacg cgatcgcctg gacgaattcc cgcctgcatc
    10501 acttcgtcac aggagcgcgc gccaatgtcc acgcgcatgg cgctgacgtc attcccctgc
    10561 cggtcgccgt caagcaggcc tggaattttg cactcttcac gggtagtgat acgcaccgac
    10621 tgaagctggt gggcagccat gcgtacattg ccaaccggca acacatcgat cgccccttcg
    10681 cgggagatgc tgcgcaccat aaatcccact tcgtccatat gcgcacagat catcaccttc
    10741 ggacctgtcg actcattgag tcggatcagc accgatccca ggccgtcaaa acggacctct
    10801 ttttgctggc gatccgcttc atccagcagg atctgccgta cttcctgttc cgaggaggcg
    10861 atcgcatctg cctcgctcaa cgcttttaag agcgataaat ccattatgcc tctcccgtga
    10921 gcaacactgt tttcggcatg gcgtagagca cttctgcgcc ttgcggggtg accagcacga
    10981 catcttcaat gcgcacgccc ccttgccctg gaaaataaat ccctggctcc acggtcagta
    11041 acatacctgg ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggttct tcatggactt
    11101 caataccgat agcatgaccg gtgttatggc cgaaaaactt gccataaccg gcctcggtaa
    11161 tcacgcggcg cgcggcatcg tcaacctgct ggcagcgcac gccggggcga attacagaga
    11221 ttgccgcgag ctgtgcctgc aggacaatct gatagacgtt aaacagcggg tgagattcgg
    11281 cgctcacccc ttcgccattc accagcaagg tgcgcgtcat atcagagcag tagccctgat
    11341 agagcgcacc gaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt
    11401 taccgtgcgg cagcgcccca cgccagccac tggcgacaat ggtatcgaaa gaggcttttt
    11461 ctgcgccctg ccggcgcata aaccactcca gttcagcggc tatctcgcat tcgctcatcc
    11521 ccgcctgaat aaagcggcga atatgctctg cgccacgatc ggcaatcccg caggcaaggc
    11581 ggattttctc cacctcttct ggcgttttga tttgccgcag cacatccggc gtggcgctaa
    11641 ccagtttcgc attgagttcg gactgccagc ggtgcgcggt ttcccaactc acctgctggc
    11701 cttcaaaacc gagcgtttgc aactgctcat cggcaatgat ttgtctgacg atggtggcaa
    11761 gcgtgtgcat cgcgtcgagc aaatgcagct ggtaaccttg cgtgcgggct tcaacatccg
    11821 cgtaatagcg cgaatccacc agaatgtgcg cactttcacg gctaatcacc acatagcctg
    11881 atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat
    11941 ccagttgttg tgccttaagc cagtcgcgca gcgaagcgag taatgtcatc tggatttttc
    12001 cttcttacag gctctcgatt aacaatttac ctttgcggaa catcatcaga cgcaagaaca
    12061 ccaccatcag cgcagtaatg attgctccca gcgcgatccc cgccatatag acacccagat
    12121 tagtgaccag cggccacgcc cagatagcgg actccgggaa ccattgcact gcgcccagcc
    12181 agacagcggc ggttgagccg acaatcgcgc cgaccatata cgacggaatg gcggtgatag
    12241 ggctttccag tgcaaacgga atcgcaccct cactgatccc cataaacgcc aggaacatcg
    12301 cggttttacc ctgcggataa agctgggcgt tgaacaagcg tttgccagtt aagcgacgat
    12361 cgataatggt cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgtgcag
    12421 tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac gaaacctacc gctttgttga
    12481 tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc
    12541 ccttctcacc tgcagcggtc agcacggtac ggataccgcc gttgatccag ccaccaaagg
    12601 gggtgatgac gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa
    12661 atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca
    12721 caaggtaacc ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg
    12781 aagaggtcgc ccattgcatc gtgctgggat cgaagttcag cagttgcgtc ggctgggtag
    12841 acatcaagcc gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca
    12901 caaaagcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca
    12961 gccaggcaaa tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat
    13021 ccatgatgcc gatatcagca ggaattttaa gccagctata agcaatcagc tgactgaaag
    13081 cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat
    13141 gctgcgggag ttcaccccag aagctggatt tagaggcctg cggattctta actgccgctg
    13201 ccgcaccgga tgcgccatgc acaacggttg cactgcgttt tttaatggcc ataacaatta
    13261 tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgattc
    13321 ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac
    13381 gttcgttatc ttccggggta actgccacgg agtggatgat gatggtcgct tcggcgatat
    13441 cctgcgccgt caggcgattc tggataccgt ccgcgccctg ggtttcaatt ttcacttcat
    13501 aaccggcttc taccgccgct tcttccagcg cctgagcggc cataaaagtg tgagccaggc
    13561 ccattgggca ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg
    13621 atagttgacg agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg
    13681 catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg
    13741 ggacacaaag aaaatttaat taactgtatg atttaaaaga ttatcgggag agttacctcc
    13801 cgatataaca ggaaggattt agagaatgtg acctaaggtc tggcgtaaat gtgcaccgga
    13861 accgagaagg cccggattgt catggacgat gagatacacc ggaatatcat ggacatattc
    13921 tttaaagcgc cctttatctt caaatgcggc gcggaaacca gaggctttga agaactcaag
    13981 gaagcgcggc acgataccac ccgcaataaa cacgccgcca aatgtcccga gattgagcgc
    14041 cagattgccg ccaaaacggc ccataatgac gcaaaacagc gacaacgcgc ggcggcaatc
    14101 ggtgcagctg tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag
    14161 gcggttgtcg gctttaacaa ttgcgcgata caaattcacc agtccagggc cagaaagcac
    14221 gcgttccgcc gaaacatgac caatttccgc acgcaatatt tcgaggataa tgccctcttc
    14281 ttcactattc ggcgcaaaat caacgtgacc gccttctcct ggcaagctta cccaacgctt
    14341 atcgacatgg accagatgcg caaccccaag ccctgttccg gcaccgtaaa ccgcaatagg
    14401 cttaccttct accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg
    14461 gatcgccatc gatacagcag taaaatcgtt aataatttcc agatggctaa aaccgagatt
    14521 ctttttcatt tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc
    14581 ggtaattggg caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag
    14641 ataaacgcga atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg
    14701 cgagatttca ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc
    14761 accgactaat gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt
    14821 ctcacactgt aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa
    14881 tatcgatctg ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt
    14941 tgcaagctgg gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc
    15001 gagccagaac catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc
    15061 ttattctgat tatggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac
    15121 tgccgggaac tctggggata gcccaggatt cacctctctg gatcatcggt gtattaacgc
    15181 taacgggtat tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagatc
    15241 ccgcctgtga accgcttatc ggcgcaccga ttccgccctc tgcgctacca ggacttatcg
    15301 tagcattaat tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcataa
    15361 ccgtcaatat cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa
    15421 tggagtggac tattttagcc tctgccggaa ccatcggcgc gctgtttggc acacctgttg
    15481 cggcggcatt gatattttcg caaaccttaa atggcagtaa tgaagttccg ctatgggatc
    15541 gtctctttgc gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc
    15601 atcctcattt ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca
    15661 gcggtgcaat tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac
    15721 cacggttgca cgcgatgatg catcaaatga aaaatccggt gttcgtgctg ggtattggcg
    15781 gatttattct cggtattctg ggggttattg gtggaccggt ttcgctgttt aaagggctgg
    15841 atgagatgca gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg
    15901 cggtgataaa acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa
    15961 tcttcccggc agtgtttgtc ggcgtggcat tagggttgat gcttcatgag cacgttcccg
    16021 ccgttccagc ggcaataacc gtttcctgcg ctatactcgg catcgtgctg gtggtaacac
    16081 gcgatggctg gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac
    16141 cgctgctctg tatcgtcatg ctcccggcat ggctgttatt agcaggtaag ccgatgatga
    16201 tggtcaatcg tcagaagcaa cagccacccc acgataacgt ttagcaaaaa atgctctcct
    16261 ttatgattta agagttatgg cgatgatacg gcgctttacc tccccgtaat attgcgttaa
    16321 caggccgctg acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg
    16381 cggccgccct gatggcattt accccgcttg cggcaaacgc aagtgaaatc accctactgc
    16441 catcaatcaa attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc
    16501 actggcgcga ccgtgactac tggcaccgca attatgaatg gcgtaaaacc gctggtggcg
    16561 tcatgataat ggctaccatc gtggctggga taagcgtaaa gcgtatgaac gtggctatcg
    16621 cgaaggctgg cgcgatcgtg acgatcatcg tggaaaaggc cgcggacatg gacaccgcca
    16681 ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc
    16741 ctacaaacca gctctttaac gcgtagggcg gataaagcat ttacgccgca tccgccaatg
    16801 gtgccaaatg cgacgctcat tcaactacaa tcccagtgcc gttcctacca gtaaccagat
    16861 attcagcgcc actaccagca ccacaatcac ccagcctgtc tgttttacgc gtttactgtt
    16921 caccagatcg cccatcaact tgctgtcact ggtgaaaatc aacagtggaa ccagcgccag
    16981 ggcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg gatctaatcc
    17041 catcagaatg acgataaatg acggcagcat ggtgactgta cgacgcaccc acagcgggat
    17101 atgaaagcga atgaagccct gcatcactac ctgccccgcc agtgtcccca ccaccgtcga
    17161 cgaaagcccc gcagccacca gacttaaacc aaaaaccgtt gccgcagcat ggcttaacaa
    17221 cggttgcagc gtcagataag cctcatcaag atcggcaacg ccagtatgac cggaaaagtg
    17281 gaacgccgcc gcagccgtag ccatcatcgc cagattgaca aaaccggcaa tcgtcatggc
    17341 gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg
    17401 ctgagtgagc gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc
    17461 cgctgccagg aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag
    17521 ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa
    17581 cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat
    17641 aaggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac caagaatgag
    17701 tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc
    17761 tgcctgaacc caatagaacc acacgacggg acgcgggtag tgatcgcgaa tctgctccgc
    17821 cagattttta ccggtggcaa tccccagttt ggccgagagg atttgaatca gcatcgccat
    17881 caggttagcc caaacgacaa cccacaataa ctgatagccg aagctggcac ccgcctgaat
    17941 attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat
    18001 taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt
    18061 catcttgtgc ctctaaaaca tagcctttgc tatgttttat gctacgttaa atgagaatga
    18121 ttatcaaatt catttaaatg ggttgtgatg atttctctga tagaccagga ttatgactac
    18181 gaaaagatag atgactcgaa tgttatggtt atgtttaata ttagcacatt tacataacct
    18241 tccgcttcca tacacaacat agcagaaatg tatgacagat cactattttt gaagcttgtc
    18301 acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg
    18361 tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg
    18421 tactggcact tgccgttgtt gcgattctcg cactgcttgt aagcagcgac cgcaaaaaaa
    18481 ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc
    18541 tgaactccga cgttggtctg ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc
    18601 tcggttttgc caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg
    18661 cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcgctg atcggtattc
    18721 tccagcatat tcgcgtattg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag
    18781 tcaacggcat gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt
    18841 ctgaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgcaat cgtatgtaca
    18901 ccatggcagc aacggcgatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca
    18961 tgctggagcc gaaatacgtc gttgcggcgc tggtactgaa catgttcagc acctttatcg
    19021 tgctgtcgct gatcaaccct taccgtgttg atgccagtga agaaaatatt cagatgtcca
    19081 acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca
    19141 aggttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga
    19201 acgcactgtt tgccaccgtg actggctggt ttggctacag tatctccttc cagggcatcc
    19261 tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc
    19321 aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc
    19381 tgcagaaaat tgcttccacg ctctctccgc gtgcggaagg catcatctct gtgttcctgg
    19441 tttccttcgc taacttctct tcaatcggga ttatcgcggg tgcggttaaa ggcctgaatg
    19501 aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg
    19561 tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta agaccataca taaaaaagcc
    19621 ggggataatt ccccggcttt tttacgcctg ttaatcagct aatggctgcg gacgaccaat
    19681 taaatatcct tgcagatatt gcaccccaag tttatgcaat agcgcctgct gctgtggcgt
    19741 ctcgacaaac tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct
    19801 cacaatcatc gcatccagcg tgttcgtgac gatatctttc acaaagacgc catcaatttt
    19861 gatgatatca gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc
    19921 atcaatcgca atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc
    19981 tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgta
    20041 ataacgctta aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg
    20101 cattaaattg actgaaaagc gtggaccttt tttgtcgcaa gggtgtgttg ccagccactt
    20161 caacaaggat tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag
    20221 gggcagaaac ttatccgggg tcataatgcc gccgtcatac ttcagtcgcg cgaggatctc
    20281 atcataacct tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg
    20341 atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt ttagtggttt gcccggaaac
    20401 catctcctct ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag
    20461 ccagcttaac tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca
    20521 ggcagcacca tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag
    20581 gatgttaacc atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag
    20641 ttcactaccc ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg
    20701 gtaaattgag cggatacagt gaacgcgcat tattaagccg taatgacgac tcataaactc
    20761 aagattatca atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg gcgcttgctc
    20821 caacgcacga aagttgggta gaagcgttaa cggatcggtc agcgcctgca aatgccagcg
    20881 gcggttaagc cattcactac gatgataaat acgcaccata tagagcaggc aaacactaaa
    20941 ggaaatcagt actgcgagaa taaatgccag cgaatattcg gttagtaccc cttgcaaaaa
    21001 gttctggttg taattcagaa ggcaaagcgt tgaaacagcc caggtgagat ttaaaaacgg
    21061 atagcgaatc ttaccgacac cgagggtaaa gatgataaag aaaacaggta ccaggtaacc
    21121 ggcaataaag tcattttcat acggtgtgca catcagaagt agcagcacgc taagagctgc
    21181 cagccagctt aaggtaaatg cgcgtttctc tttgctcaac gacggagcga tatccctacg
    21241 ccacaatatc tgtgcaaagt ggggacttac aatcatgcgg gtgagatagt aaaaaagcat
    21301 gttgtaaatc agcactgcgg tgaaaaggct tagcaaatca acgaccgtga aaatggcatc
    21361 cgcatcgccg aaaaaggtag atatctttag cggaaaatca aagaaatttc ccacaagata
    21421 catgctgcat ttgatgccaa tcggcgccac caaaccaagc cagaacagac gttgccagac
    21481 gtatcggctg gtcaatccat aacgccaacg cgtccccaat tgccagcgta atattgcaca
    21541 ggcacacagg actgcaaacg cctggctgcc gatcatgacg gcggtctgta aaaaagagag
    21601 atgaaggttc cacagattgg tgcaaaacat cccgcataaa atgggaacga caccacgcca
    21661 gccaaagata aacaagattg acagcattac gcacagtggc atccacgcca gaaagatata
    21721 attggaatgg ataacagcaa gtggcgaaat taaacgggat agctgaataa gtaccacggt
    21781 gagcgtaaac gctagtgtga atatcttgat attttttatc aggttatgct ccacaaacat
    21841 gagtacacta ctctcactga tcgggttttc aggcacacaa ataataagtt tattcgcatc
    21901 gcgaagcaaa caggggccag cctgttgctg ttttttcagt cagtgatgca ttcaccacac
    21961 ggcactgcca ggatgtagac tgtattacaa aagcggcaaa aggcagagac aaaaaacccc
    22021 cgctttgcag cgagggtttg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
    22081 tgcatgccat gcaagcgctc tcccagctga gctatagccc catcacgtaa agcttgtcga
    22141 gttgacgggc ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgccacgtgt
    22201 agtttcaatc gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt
    22261 agttattcat gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc
    22321 acatacattc agggaattgt tatgttcaag gagaggatga cgccagatga acttgccaga
    22381 ctgaccggct atagccgcca gaccattaat aaatgggtac gcaaggaagg ctggacaacg
    22441 tcaccaaaac caggcgtcca gggtggcaaa gctcgactgg ttcacgtcaa tgaacaggtt
    22501 cgtgaatata ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc agccctttct
    22561 ggtgatgcgc cgcttgaagt gttgctggtg acactggcga aagagatgac gccagttgaa
    22621 caaaaacagt ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta
    22681 gggattcgcg atagcaaata atatgaaaag attacgcaat aaaatgacca ccgaagaact
    22741 ggctgaatgc ctcggcgtgg ctaaacaaac tgttaaccgt tggatcagag aaaaaggctg
    22801 gaaaacggaa aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac
    22861 gcaagtttgc gagtttattc agaacacgcc agccttccat aacacaccga tgcttctgga
    22921 agccgaagaa ccccttgccg aatacgcacc gggtattcgc actcctgctt atcggcaaat
    22981 catcagcgca atagacaaca tgactcacag tgagcaagaa aaagttgcgc aatttttgtc
    23041 acgcgaagga attcgtaatt ttcttgctcg tctcgacata gatgaatcag cataaataaa
    23101 aaacggcagg ggtatctctc ctgccgttta tcttttatgc gctaattact gctgattttc
    23161 gcgttcagca ataaaagcca gcgctttgtt gatacgctcg atactgcggg tcttaccgat
    23221 tgcgtgaacg gtaacatcca gcgccggaga ctgccccgca ccggttacgg cgacacgcag
    23281 cggcatacca actttaccca tacccacttc cagctcatcc gccgtcgcct gaatagcgtg
    23341 atgaacgttt tcagcggtcc agtcagtaat cgcagtcagt ttgtcacgaa ctacttccag
    23401 cggctgacgc gctaccggac gcagatgttt tttcgcggcg tcggcatcga actcagcaaa
    23461 atcttcgtag aaataacggc agctctgtgc catctctttc agcgtcttgc agcgttcgcc
    23521 cagcagtttc accagatcag ccagctgcgg gccgttacgg gtatcgatat tttcctgctc
    23581 aatgtgccac tgtaagtgag tagcaacata ctccggcggc aacgcgttaa tgtagtgatg
    23641 gttcagccac agcagcttgt cggtgttgaa cgcactggca gatttgctga cggcattcag
    23701 agtgaagtat ttaatcatct cttcacgagt gaagatttcc tgatcgccgt gggaccagcc
    23761 cagacgcacc agatagttca gcagtgcttc tggcagataa ccatcatcac gatactgcat
    23821 tacgctgacc gccccgtgac gtttggacag ttttttaccg tcatcgccgt tgatcataga
    23881 aacgtgcgcg taaaccggta ccggcgcttt cagagcctta agaatgttaa tctggcgcgg
    23941 tgtgttgttg atatggtctt cgccacggat aacgtgggtg atttccatat cccagtcatc
    24001 gacaaccaca cagaagttat aggttgggga accatcggta cggcggataa tcagatcatc
    24061 cagctcctgg ttgctgaact cgatcggacc acggatctga tcgtcaaaaa caacagaacc
    24121 ttcctgcggg ttagcaaaac gtacgacaca cggctcatca tcagcgtgat gctcatgact
    24181 gtggcggcag cgaccgtcat aacgcggctt ctcacctttc gccatttgct cttcgcgcag
    24241 cgcttccagg cgctctttag agcaatagca tttataagct gtgccctctt ccagcatctg
    24301 atcgatcacc gcgttgtagc gatcaaaacg tttggtctgg tagtacggac cttcatccca
    24361 ctccaggttc agccagttca tgccatccat aatggcttcg atagcttccg gcgtggaacg
    24421 ctcaagatcg gtgtcttcaa tacgcagcac gaactcaccg ccgtggttac gtgcaaaaag
    24481 ccaggagtaa agagcagtac gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc
    24541 gaagcgagtt ttgattttca tgaaatggcc ttacgtttag aaagatgccg acaaccggca
    24601 aatcctggaa aaattgagtg ggcgatattc tatcactccc gcctgattcc tcaatgtagt
    24661 tcgggttgca accttacgca ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt
    24721 tgcgatcatc ttgcttaaat ttacgacgaa cgaacaattt ctttagaaaa tgcgttgact
    24781 cattttgaac tctccctata atgcgactcc acacagcggg ggtgattagc tcagctggga
    24841 gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccaactac
    24901 tttatgtagg ctccgccgtg tagcaagaaa ttga
//
LOCUS       DADTFG010000067        24682 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.067, whole genome shotgun sequence.
ACCESSION   DADTFG010000067 DADTFG010000000
VERSION     DADTFG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.067"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(3..416)
                     /locus_tag="J1138_002494"
     CDS             complement(3..416)
                     /locus_tag="J1138_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454273.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRL
                     LRQEEQERRKNIEKLIQIERNAKARISRVTKSRTE"
     gene            complement(417..842)
                     /locus_tag="J1138_002495"
     CDS             complement(417..842)
                     /locus_tag="J1138_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBA8454274.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKKTPVVDPDLIWSLPD
                     GEIRRYDRRQNIICSECRKSEVMQRVLVFYQGNFQEVVL"
     gene            complement(883..1968)
                     /locus_tag="J1138_002496"
     CDS             complement(883..1968)
                     /locus_tag="J1138_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA8454275.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGPEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSAFYDRYKTDFHLDVRRNNRRNSARGFEEALKGLLSQ
                     EQEQEKEQDQDKNTMLHGEKIATNQAGDVQNVNPGQPAGTTPEADSGAVQQAMTARPE
                     QSHQLQQPEADSAIQREADQVVPENTGQPVRRVDYPDVFEQVWREYPLRAGANPKKSA
                     FSAWKARLREGVPPEAMLDGVRRYARYLATTGKTGTEFVQRATTFFGPDRNFENPWLL
                     PVSGTNNQRCVNHVSEPDTEIPPGFRG"
     gene            2048..2443
                     /locus_tag="J1138_002497"
     CDS             2048..2443
                     /locus_tag="J1138_002497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454276.1"
                     /translation="MDFATITSSVVLSACVAGVVSLVNGAWQRKSERTIEAQRRAAEA
                     RAKIREMALTLALKEWELHQTISKAKGYGVSGPEVYVFRYFRMLNLMEEDKFTIENLR
                     LIQYDSMCAVAAIQAEIERYREKNGLPMP"
     gene            complement(2678..3103)
                     /locus_tag="J1138_002498"
     CDS             complement(2678..3103)
                     /locus_tag="J1138_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBA8454277.1"
                     /translation="MKISHEHIRMAMNAWAHPDGEKVPTAKITKAYFELGMTFPELYG
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVVMH"
     gene            complement(3087..3410)
                     /locus_tag="J1138_002499"
     CDS             complement(3087..3410)
                     /locus_tag="J1138_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8454278.1"
                     /translation="MKKENYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATRGGVLCEELRPDVDWSYLRRSSCYSLNMLMKQPNDENEHTRNIKRQMI
                     HENQP"
     gene            3536..4012
                     /locus_tag="J1138_002500"
     CDS             3536..4012
                     /locus_tag="J1138_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8454279.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGSVKSKAEVARHFKVQPPSIYDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTSLISDTKSELLDINSLYQAASD
                     EKRAIVAFLLSENATEPGWVDHDVRAYIATMEMKVGKYLKDQESERKSQNITKTGT"
     gene            4333..4485
                     /locus_tag="J1138_002501"
     CDS             4333..4485
                     /locus_tag="J1138_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HBA8454280.1"
                     /translation="MDTLNLSNNESLVCGVFPNQDGTFTAMTYTKSKTFKTAAGAHRW
                     LARNTD"
     gene            4485..4856
                     /locus_tag="J1138_002502"
     CDS             4485..4856
                     /locus_tag="J1138_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454281.1"
                     /translation="MRLTMEFKYLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMAR
                     NVRDAFTGLFSDTALNIHGTEDTAKQFAEKEVEDLSSKSQKQPNNHEINELLLATGFI
                     NINEYERRKKMLSDQSIKISF"
     gene            5572..5760
                     /locus_tag="J1138_002503"
     CDS             5572..5760
                     /locus_tag="J1138_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor"
                     /protein_id="HBA8454282.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     AILSRIALIKLGIFGERQ"
     gene            5757..5945
                     /locus_tag="J1138_002504"
     CDS             5757..5945
                     /locus_tag="J1138_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HBA8454283.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            6041..8512
                     /locus_tag="J1138_002505"
     CDS             6041..8512
                     /locus_tag="J1138_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBA8454284.1"
                     /translation="MSKIFICVAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDSAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDVASASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDIATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPAAKWPEIQAEMRIWKKRREGERKETGKYTSVVDIARS
                     RVNQQNTENSAEKTGAVTVAVRREYKQTWKTLDHELACALWPGDVNAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSADALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGATEISKEEYFARKNGTYTGLPQNVDTAEDSVQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNEPKWVKGIQSRNAE
                     DQNQPGVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDGESLVEVQENAPEEMEGTEDPHKENTDSDQYHASDNKTGETANPL
                     IKVNGHREITSTSRLWHHLMIDLETMGKNPDAPINAIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIERDTIKWWLKQSREAQSAILTDEIPLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGEHHNA
                     LHDTRYQAKYVSAIWQKLIPSQADF"
     gene            8574..8843
                     /locus_tag="J1138_002506"
     CDS             8574..8843
                     /locus_tag="J1138_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBA8454285.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKMGYIVPRPQKIRG
                     RWLIDRRAVFVGPSETGIAPEIHTDDDDALKEILTHVTEATKKQH"
     gene            8812..9930
                     /locus_tag="J1138_002507"
     CDS             8812..9930
                     /locus_tag="J1138_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8454286.1"
                     /translation="MSPRPRKNSTDIACLYEKFDRRTGKVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDSRLKN
                     GDIAATTHKEKARMAAYLVSRLGNHPLKDLEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLALEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKDVIDRC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWIKLVI"
     gene            10097..10891
                     /gene="potC"
                     /locus_tag="J1138_002508"
     CDS             10097..10891
                     /gene="potC"
                     /locus_tag="J1138_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBA8454287.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            10888..11934
                     /gene="potD"
                     /locus_tag="J1138_002509"
     CDS             10888..11934
                     /gene="potD"
                     /locus_tag="J1138_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBA8454288.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            11992..12453
                     /locus_tag="J1138_002510"
     CDS             11992..12453
                     /locus_tag="J1138_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HBA8454289.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            12450..13238
                     /locus_tag="J1138_002511"
     CDS             12450..13238
                     /locus_tag="J1138_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBA8454290.1"
                     /translation="MKKIMILLSLLMFLPLTAISKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVVPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQSINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(13364..14185)
                     /gene="cobB"
                     /locus_tag="J1138_002512"
     CDS             complement(13364..14185)
                     /gene="cobB"
                     /locus_tag="J1138_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBA8454291.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(14201..15112)
                     /gene="nagK"
                     /locus_tag="J1138_002513"
     CDS             complement(14201..15112)
                     /gene="nagK"
                     /locus_tag="J1138_002513"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBA8454292.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(15141..16385)
                     /gene="lolE"
                     /locus_tag="J1138_002514"
     CDS             complement(15141..16385)
                     /gene="lolE"
                     /locus_tag="J1138_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBA8454293.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLKLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(16385..17086)
                     /gene="lolD"
                     /locus_tag="J1138_002515"
     CDS             complement(16385..17086)
                     /gene="lolD"
                     /locus_tag="J1138_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBA8454294.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(17079..18278)
                     /gene="lolC"
                     /locus_tag="J1138_002516"
     CDS             complement(17079..18278)
                     /gene="lolC"
                     /locus_tag="J1138_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBA8454295.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            18540..19613
                     /locus_tag="J1138_002517"
     CDS             18540..19613
                     /locus_tag="J1138_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBA8454296.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERNLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFVLFVLMSVAVNFVPTPWWGMNSVSRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLAVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            19741..23187
                     /gene="mfd"
                     /locus_tag="J1138_002518"
     CDS             19741..23187
                     /gene="mfd"
                     /locus_tag="J1138_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBA8454297.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTMRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVDLRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            23330..24292
                     /gene="ldtC"
                     /locus_tag="J1138_002519"
     CDS             23330..24292
                     /gene="ldtC"
                     /locus_tag="J1138_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBA8454298.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSADSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(24375..24632)
                     /gene="bhsA"
                     /locus_tag="J1138_002520"
     CDS             complement(24375..24632)
                     /gene="bhsA"
                     /locus_tag="J1138_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBA8454299.1"
                     /translation="MKNVKNLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6445 a         6318 c         5986 g         5933 t
ORIGIN      
        1 aattattcag ttcttgattt tgtcaccctg cttatccgcg ctttcgcgtt acgctcaatc
       61 tgaattagct tttctatatt ttttcgcctt tcctgttcct cctgacgcaa tagccttaca
      121 tcatctgcca gtctggtttc tcttttcgcc acagagagca tccagtcaaa tggctccaca
      181 actgcaccgc agattttaca gcggacctga cgctcttttt cgtcaacccg gacagaggcg
      241 tgatgacaat atggtctttc cgatggctca taaagaaaat taacctgatt acgtgggtca
      301 tcctctttta ccggaaataa aacaatatta cttaactcat cttctggttt tatttccatg
      361 ctcctctcct ttgatgcgaa tgccagcggc aattgaagcc tgatagctaa tttcactcac
      421 agcaccacct cctgaaaatt cccctgataa aataccagca cgcgctgcat aacttcgctc
      481 ttccggcact cgctacagat tatgttctga cgcctgtcat aacgacgtat ttctccgtct
      541 ggtaacgacc agataaggtc cggatcaacc acaggtgttt ttttcttcag ctttgccctt
      601 gagagttttt tgcgggcgtt ttgccagtcc ttacgagcct gttcagacgg gaataacccg
      661 taaccagagt tgtatacatc accactggca accagctctc tggcgagaac gctcatcaga
      721 tatcttgtcg cacctgtctt ggcttccagt tgccgtaacg tctcgcgccc actccggcgt
      781 actagctcaa caacctgccc tttaattttt tctcgctctt cttgtgtaaa aacttttgcc
      841 acaagccctc ctgaaaatta cctcatgacc tgaaataaac acttaccccc tgaagcctgg
      901 cggaatttcg gtatccggtt cagaaacatg attcacacaa cgctggttgt tcgtgccgct
      961 taccgggagc aaccaggggt tttcaaaatt ccggtccggt ccaaaaaacg tcgtcgctcg
     1021 ctgaacaaat tccgttcccg ttttcccggt agtcgccagg tatcttgcgt aacgcctcac
     1081 gccatccagc atggcctctg gtggcacccc ctcgcgtaat ctggccttcc aggcactgaa
     1141 agcggatttc ttcgggtttg ccccggcacg caacgggtat tcccgccaga cctgttcgaa
     1201 cacatccgga taatccactc gtctcacagg ctgcccggtg ttttccggga ctacctgatc
     1261 ggcttcccgc tgaatggcgg aatcggcttc aggctgctgc agttggtgtg attgttccgg
     1321 ccttgcggtc atcgcctgct gcacagcgcc cgaatcggct tccggtgtcg tgcctgctgg
     1381 ctgaccagga ttgacgttct gaacatcccc tgcctggttt gtggcgattt tttcgccatg
     1441 aagcatagta tttttatcct gatcctgttc tttctcctgt tcctgttctt ggcttagaag
     1501 cccctttaaa gcctcttcga agccccttgc tgaattccgg cgattattcc gcctcacatc
     1561 cagatggaaa tccgttttat atctgtcgta aaaggctgac agaaaggcgt tttcaggtaa
     1621 tgatgcatac tcattcctga cacctgcaca acggttatct ccaggcttca acgtttcccc
     1681 aacctgccag gctgccattt catggaccca gaccatctcg gcatcatggt catagctaca
     1741 aaaaccagct tcaacagccc ttttaagccc cttcgaagcc ccttccggac caagcccggt
     1801 ttcatgagca aggtacaaaa ctggcaggta atacaaaccc aacatatttg cgtgagggga
     1861 tgtcatcagg taaaaagcaa caacttgcgc ttctgcaccc gcctttctaa gttctctccc
     1921 cgtttctccc agccagaatc gcggggaaac ttttgcataa tcacgcatgt tcacctcgtc
     1981 tggagccaaa ccttcctctg gttataatct gtgattcacc aatcaacaga accagaggag
     2041 gttcgacatg gattttgcaa ctatcacaag tagcgttgtg ttgagtgcgt gtgttgctgg
     2101 cgtggtttcg ctggttaatg gtgcctggca acgcaaatcg gaacgaacca ttgaagcgca
     2161 acgccgggct gctgaagcca gagcaaaaat aagagaaatg gctctcactc tcgcattgaa
     2221 agaatgggag ctccaccaaa ccatatcgaa ggcaaagggg tatggtgtca gcggcccaga
     2281 agtttacgtc tttcgatact ttcgtatgct caacctgatg gaggaagaca aattcacgat
     2341 tgaaaatctc agactgatcc agtacgactc catgtgtgct gttgcagcaa ttcaggcaga
     2401 gatcgagcga tatcgggaga aaaacggcct cccgatgcca taaacaatca gcatttttca
     2461 tctttccaaa tcctcatatc aggcttgcgc ccaatgaacc gacgaagaca atttaaagtc
     2521 catcgggtga agtaatccct gaaaccaaag agataccagg tgaacagatt cacgataaaa
     2581 tgtccggcca gagccccacc aataccacag gcaagaacag taaaaaaatc aggtagattc
     2641 acagccccct ccgtcattgt taacgccatc cgtgctttta gtgcatcacc acggcatttc
     2701 ctgccgggcc accacggttc atctgattga aaccggcaat cgccactgcg acaaaatcat
     2761 cagcgtctct caccagtcgt tcccgcgtct ccaccagctc ccgaaaatag gctgaactgt
     2821 ggctgcgcat tcgggccacc agcaaaggtg gcattgcctt ttcgatcgct ggtaacaacg
     2881 cctgaatttt ttcaacagca tcaggggtgt ctttctctac ccagcggaaa attttctggg
     2941 tattacgagc tagggcttcc ggatggctgt cgccgtacag ttccggaaat gtcattccca
     3001 gttcaaaata cgctttggta atcttcgcag ttggtacttt ctcgccatcc ggatgcgccc
     3061 aggcattcat cgccatgcgg atgtgctcat ggctgatttt catgaatcat ttgcctcttg
     3121 atatttcggg tatgctcatt ttcgtcattt ggttgcttca tcaacatatt cagcgaatag
     3181 catgacgagc gtcgtaagta tgaccagtca acatcaggac gaagttcttc gcacagaaca
     3241 ccacctcttg ttgcacgttc aattgctgga catcgctcag caggcaactg acgtacacct
     3301 ttgatccatt gatttacgct tggaggtgat acacctaaaa gcctagccat tgcagattgc
     3361 ccaccgacaa cagcacacgc tcgtttgaat gaatagttct cttttttcat cgaatgaact
     3421 ccaaaaaaac acgaaacaat attaggcgac gcctaatgca aatgtcaata ggcgtcgcct
     3481 aacgcaaatg aagtaggtat tgcctaattc aatgagcata ggagagtatt aagcaatgct
     3541 tagtggtaaa gacttaggcc gagcgataga gcaggccatt aacaaaaaaa ttgcatcagg
     3601 atccgtcaaa tcaaaggcgg aggtagcgcg tcacttcaaa gtccaaccac catcaatcta
     3661 tgattggatt aagaaaggtt cgataagtaa agataaactt ccagaactat ggcgtttctt
     3721 ttctgatgtg gttggcccag agcattgggg gcttaacgaa taccccatac caacctccct
     3781 catttcagat acaaaaagtg aacttttaga cataaacagc ctttatcaag ccgcctctga
     3841 tgaaaaaaga gccattgtgg cttttctctt atcagaaaat gccacggaac ctggttgggt
     3901 tgatcatgac gttcgcgcct acattgccac aatggagatg aaggtaggta agtatttgaa
     3961 agatcaagaa tcagaacgga aaagccagaa catcaccaaa acgggaactt aaacttatat
     4021 ggtctgatgg gaacttccta gagcccgtta tctagccccc ccatcacctc ttccacatat
     4081 catcgcctat taggtttgca tcaaaaacat taggcgcagc ctattgacaa ttaattaggc
     4141 attacctata gttctcccat accaacccac cccgcccaca gaacaccggg caatacttcg
     4201 agttacccgg cagtgattag gggttaagta gccagcccga ggcgtatgaa catgacggcg
     4261 ggaacacttt gtataacagc gcagcaggtt tttagttccg ctaccccggc gttaagggga
     4321 aatgaggtca acatggatac gctcaatctt agcaacaacg aatctctggt atgcggtgtg
     4381 ttccctaacc aggacggcac cttcaccgcg atgacgtata ccaaaagtaa aacgtttaaa
     4441 accgcagctg gcgcgcatcg ctggttagca agaaacactg actgatgagg ttgacgatgg
     4501 aatttaaata tttaccgcct tcaatccagg agattgcagc acacacactt cgtcatcgtc
     4561 tgaacgaact tgaattggaa tcggtaacaa aaaaagacac tgataatatg gctcgtaatg
     4621 tgcgcgatgc gtttactggg ctgttttcag atacagcttt aaatatccat ggcacagagg
     4681 atacagcaaa acaatttgca gaaaaagaag tggaagatct cagttcaaag agccagaaac
     4741 agccaaacaa ccatgaaatt aacgaactgc ttctggcaac aggcttcata aatattaacg
     4801 aatatgaacg ccgtaaaaaa atgttatctg atcaatccat caagatatct ttttagcatc
     4861 agcgtttcta cgttattaaa accatcagat tttaacatag cttcgattcg ctgatttaaa
     4921 ttgccaacta actgttgata ctgagtagat ggaagcatgt gtaccagctc cttgagaaca
     4981 caataacatg caccaagttt taactcctgc tctgacattt tatcctccat tgaggttact
     5041 ggttgagaat ggagacctct cgtgacagcg tgtggtcgtg cgccggacac ggataagaat
     5101 ccggcactga cagtttactg aaaggatata tccctgaaaa gtcagggcat aacacgaaag
     5161 cgcacggcga gattcctttg catataaggc ttgtcgttaa atttcttcaa ccgtgcgttt
     5221 ccggttgtgg caatccgcga aatagcgcgg cggtaagtat ggcggggtta ttccttcccc
     5281 cgttgaggac accgggttgt caggttgacc atacgcttaa gtgacacccc agccacaaca
     5341 acctctgtta tcacttttct ggtgattcgg cggaaatgga tatccgctct ttttaaagtg
     5401 aattttgtga tgcggtgaat gcggccatgc gcacgcggaa cagttaaaac aagcggtctt
     5461 taacttgcgt aacagacatc aactaacaat ccggcgttaa ttgttaactg gttaacgtca
     5521 cctggaggca ccaggcacgg catcacaaaa ttcattgttg aggacgcgat aatggaaacg
     5581 ttattaccaa acgttaatac gtctgaaggt tgttttgata ttggtgttct gctcagtaat
     5641 aaagcgttta ctgaggatgc cattaatatg cggaaatatg agccttatct gctcaatgat
     5701 aatgccattc tttcccgaat tgcccttatt aaacttggta ttttcggaga acgtcaatga
     5761 cttcagcatt tgcactggtg atgacggttt ttcttataac gggtgaatca cagaatgtga
     5821 ttaccggaat ttatgccagt aaagaatcct gcctccaggc aagagacgag caaaaaattt
     5881 ctggtgaatg cctcccggta aaaaaagtat cgctgtacct gaataacgaa acaccggctg
     5941 gataaccctc cagccatatt aacgccatac caacggatta aaaatgccag caatggcagg
     6001 gattcgttca ccctgaaatc tgtaatgagg ttaaaacaca atgagtaaaa tctttatttg
     6061 cgtggctatt cctgatgaac aggccataaa agaagatagc gctgttgcgg tggccactgc
     6121 cattgaagcc ggtgatgagc gtcgcgcacg cgcaaaattt cactggcaat ttctggagca
     6181 attcccggca gctcaggaca gcgcttataa atttattgtc tgtgaggata aacccggcat
     6241 accccgccct gccctcgatt cctgggatac cgaatatatg caggaaaacc gctgggatgt
     6301 ggcgtctgct tcctttgtcc cggtcgagac tgaatccgat ccgatgaacg tcacctttga
     6361 caagctggcc cctgaagtac agaacgccgt catggttaag ttcgacacat gtgaaaacat
     6421 cactgttgat atggtgatta gcgcacagga attgttgcag gaagacattg caacattcga
     6481 cggccatatc gttgaagcgt tgatgaaaat gccagaagtt aacgccatgt atccggagct
     6541 taagctgcat gccatcgggt gggttaagca taaatgcaag cctgctgcta aatggcccga
     6601 aattcaggca gagatgcgca tctggaaaaa acgtcgcgaa ggtgaacgca aggaaaccgg
     6661 gaaatacaca tctgtcgttg atatcgcccg ttccagagta aaccaacaga acactgaaaa
     6721 ctctgctgaa aaaaccgggg ctgtcactgt tgccgttcgc cgagaataca aacagacatg
     6781 gaaaacactc gaccatgagc tggcctgcgc cctctggccc ggtgatgtga atgcgggaaa
     6841 tattgacggt accatccatc gctgggcgac aaatgaggtt atcgacaagg atcgcgaaga
     6901 ctggaagcgt atctcagcat caatgcgcaa acagcccgaa gcacttggct atgaccgtca
     6961 gactattttt ggccttgtcc gtgaacgtcc gatcgacatt cacaaagatc ccgttgcact
     7021 gaacaaatat atcagcgaat acctgacaac aaagggcgtg tttgagcatg aagaaacaga
     7081 ccagagctct gctgatgctc tccagccgtc agcagcacaa actgctccag tggagacggc
     7141 agaatccgat actcaaaaaa atgaaatcct ggtggaagct gaaccatctg tagagcgtga
     7201 aggaccattt tatttcgtct ttaccgataa gggcggggaa aaatacggca gggcaaacaa
     7261 actttctggt ctggacaagg cgctggctgc cggcgctacc gaaatctcaa aagaggaata
     7321 ttttgcccga aaaaatggca catatacggg cttaccgcaa aatgtagata ccgctgaaga
     7381 ttccgtacaa ccagagccgg taaaagttac cgctgacgaa gtaaacaaaa ttatgcaggc
     7441 agccaatatc agccagcctg acgccgataa attgcttgct gcatcacgtg gtgaatttgt
     7501 tgaagggatt agcgacccta atgagccgaa atgggtgaaa gggatccaga gtcgcaacgc
     7561 tgaggaccag aatcagcccg gcgtgaaaca aaatgagcca gaagcggaac aaaacagccc
     7621 ggatacgcaa caaaacgggc cagaagagca acaaccagaa ccagcagtgc aacaggaagt
     7681 ggagaaagtt tgcaccgcat gcggtcagac cggtggcggc aactgtcctg actgtggtgc
     7741 ggtgatgggc gacgcaacct accaggaaac attcgatgga gagagcctgg ttgaagttca
     7801 ggaaaatgct ccggaggaaa tggaaggcac tgaagatccg cacaaggaga acactgacag
     7861 cgatcagtat cacgccagcg ataataaaac tggcgagaca gcaaatccct taattaaggt
     7921 gaacggtcat cgtgaaatca catccaccag caggttgtgg caccatctga tgattgacct
     7981 tgaaacaatg ggaaaaaatc ctgatgcgcc aataaacgct atagccggta agttttttga
     8041 tccggcaacc ggagagatgg ggccagaatt cagcaaaact atcgatctgg aaaccgcagg
     8101 cggggtcatc gaacgggaca ccattaagtg gtggctgaaa cagtcacgcg aagcacaatc
     8161 cgccattctg accgatgaaa tcccgttgga tgatgcactg ctgcaattcc gggaatttat
     8221 cgacgaaaac tccggtgaat tttttgttca ggtctggggt aacggtgcaa ctttcgacaa
     8281 cgtgatttta cgccgttcat atgaacggca ggggatcccc tgcccgtggc gttacaccaa
     8341 tgatcgcgat gtaagaacga tggttgctct gggactggtg atggatttcg acgcaagaac
     8401 gactattcca ttcgaaggtg aacaccataa cgccctgcac gatacgcgtt accaggcaaa
     8461 atacgtttca gccatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
     8521 cgtcgccagt tgtagttgct attctgcaac tggcgcattc cggagtgatg gccatgagcg
     8581 aacagtacct gataacgctc gacgagtgga aacctaaacg gttcagtctc ccaataacaa
     8641 acactaccct ggtgaaatac ggaaaaatgg gatacatcgt tccaaggcca caaaaaatcc
     8701 gtgggcgttg gctgatagat cgccgagcag tatttgtggg tcctagtgaa acaggaattg
     8761 cgccggaaat tcatactgac gatgatgacg cactgaagga gattttaact catgtcaccg
     8821 aggccacgaa aaaacagcac tgacatagca tgtctttacg aaaagttcga tcgcagaact
     8881 ggcaaggttt actaccagta taaaaatcca gtgactggaa aatttcacgg actcggaaca
     8941 gacaaaggca aggcagaaaa aatcgcttcc acggccaatc aacgaatagc tgcagcagaa
     9001 gcagaatatt tcatgcgcaa aattgatgaa agtccatcag caacaaaacg tcggggtatc
     9061 agattaaaag catgggttga tcgatatctg aaaatacagg actcacgact gaaaaatgga
     9121 gatattgcag ctacaaccca caaagaaaag gcgcgaatgg ctgcatacct ggtttcccgc
     9181 ctgggaaatc acccattgaa agacctggaa gtaagagact ttgcattaat actggatgaa
     9241 tggctggata aagacatggt cagcacagcg agagtaaatc gcggattatg ggttgatatt
     9301 tataaagaag cacaacatgc aggggaagtt cctcctggat ggaatcctcc ggaggctacc
     9361 cgtaaaccga tccctaaagt aaccagagcc aggctcgccc tggaagactg gcaaaaaatt
     9421 tacaacgcaa cgccggaaaa acactttatc cgtaacgcaa tgcttcttgc gattgtcact
     9481 ggtcagcgcc gtgatgacat ttgccacatg cgtttttcag atgtgtggaa cgaacacttg
     9541 catatcaccc agggaaaaac cggaatgcgt ctggcgttac cgcttacact acgctgtgat
     9601 gccattggga taacgttaaa agacgttatt gatagatgcc gagacagaat attaagtcca
     9661 tatctaatcc atagtcggca ccagaaacaa ccaaagccga tgagtaaaga caacctgagc
     9721 gactactttg ccaaagcgcg ggatctggct ggaataattc caccagcagg aaaaacaccg
     9781 cccacatttc atgaacaacg ctctctatca gaacggctgt accgtgcaca gggtatcgat
     9841 acaaaaacat tactaggaca taaagtccag gcaaccaccg atcgctataa cgatacccga
     9901 ggtcaggaat ggattaagtt ggttatttga caaacactat atggaatatc cgtcaccatc
     9961 tcaggaaaaa agtgttgtaa aatgcgtggc cttgttttgg ggaaaagttt tggagagatt
    10021 ttggagaaag ggaaaaattg aataaattca aaatcctgaa agtgctccac cttatgaata
    10081 agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga ccgctatcta
    10141 cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca gctcgcgctt
    10201 tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga acaacgacag
    10261 cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga cgtttgctac
    10321 gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgtg gtaagccgtt
    10381 cgttagcgga atgctgtttg tggtgatgat gtcaccagat atcgtgatgg cgatttcgct
    10441 gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc tgttctcgca
    10501 tatcaccttc tgcctgccat ttgtggtggt gacagtgtat tcgcgcctga aaggttttga
    10561 cgtgcgaatg ctggaagcgg caaaagatct cggtgccagc gaatttacca ttctgcgaaa
    10621 aatcattctg ccactggcaa tgccagcggt agcggcgggc tgggtgttaa gctttaccct
    10681 gtcgatggac gacgtggtgg tttcttcgtt tgtcaccggg ccgagttatg aaattctgcc
    10741 gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg cgctggcaac
    10801 catattactg gtgctgtcgc tggtgatggt aattgccagc cagcttattg ctcgtgataa
    10861 aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct gctcgcggcg
    10921 ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac gctgtatttc
    10981 tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa agaaaccggt
    11041 attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgcgaa gctgaaaacg
    11101 tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga taaaatgcgt
    11161 aaagaaggga tgatccagaa gatcgacaag tcgaagttaa gcaatttcag caacctcgat
    11221 ccagacatgc tcaacaagcc gtttgacccg aataacgact attccattcc gtatatctgg
    11281 ggtgcaacgg cgattggcgt taacggtgat gcggtggatc cgaaatctgt caccagctgg
    11341 gccgatctgt ggaagcctga gtacaaaggc agcctgctgc tgaccgacga tgcccgtgaa
    11401 gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga tccgaaagag
    11461 attgaagctg catacaacga gctgaaaaaa ctgatgccaa acgtcgcggc gtttaactcc
    11521 gataaccccg ctaacccgta tatggaaggc gaagttaacc tcggcatgat ctggaacggt
    11581 tctgctttcg ttgcacgcca ggcgggtacg ccaattgatg tggtatggcc gaaagaaggc
    11641 ggcattttct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa agaaggcgcg
    11701 ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc tgaaactatc
    11761 ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccggaagt ggcgaacgat
    11821 aaaacgctct acccggatgc tgaaaccatt aaaaatggtg aatggcaaaa tgacgtaggc
    11881 tccgccagca gcatttatga agagtattat cagaagctga aagcaggacg ttaattcagc
    11941 aagtgggcgg tggtattacc gcccgttttt tatttagcaa ggatgtggct aatgagccag
    12001 gacagtagag ttttctttcg tatattcttc ggtatcggtc tggtactgat cctcatttct
    12061 gttgttatct tttataacca gttcacctat agcaaagacg cgattcatac tgaaggcgtc
    12121 attgttgata ccgtctggca ctccagtcat tctcatcgca cgggtaaaaa tggttcgtgg
    12181 tatcccgtcg ttgcctttcg tccgacgcca gattacacgc taattttcaa ttcgagtatt
    12241 ggcagcgatt tttatgaaga cagcgaaggc gataaagtta atgtttatta ttctccagga
    12301 catcctgaaa aggctgagat aaataacccg tgggttaatt tttttaaatg ggggtttatc
    12361 ggcattatgg gcgttatttt tatcgccgtc ggtttattaa tcagtatgcc ctcctctaaa
    12421 aagacacgca ggaaacggaa gtcccgccca tgaaaaaaat catgatactg ctcagtttac
    12481 tgatgttcct gcctctgacg gcaatcagta agcccttaat tccaataatg aagacacttt
    12541 ttacggatgt taccggtact gtaccagatg ctgaagagat cgcgcataaa gccgaacttt
    12601 ttcgccaaca aaccggagtt gtaccgttta ttgtggtttt acccgacatt aataatgaag
    12661 ccagtctcag gcaaaatggc aaagcgatgc tggcgcatgc ggcatcttca atgagtaatg
    12721 taaaaggaag tgttctgtta ctctttacta cccgcgagcc acggttaatt atgataacca
    12781 acggccaggt cgaaagcagt atggacgata aacatctcgg ccttctggta gaaaatcaca
    12841 cgctggctta tttacatgcg gatctctggt atcagagtat caataatgca ttggctgttt
    12901 tacaagcaca gatattaaaa caaccgacgc caccactgac atattatccg catcccggac
    12961 aacagcatga aaatgatcca cccggcagta ccacgacatt aggccttttt gcctgggcag
    13021 ttgcattcat cgtctttgct gcatttttta actacacaac gcgcctttat tatgcgttaa
    13081 aatttgcagt ggcgatggct gttgccaata tgggttatca ggctttatgt ttgtatatcg
    13141 ataacagctt tgcgattacc agaatatcgc cactctgggc agggctgatt ggtgtctgta
    13201 cgtttattgc cgcgttacta tggacgagta aacggtaaaa gaggcagtaa agttgccgga
    13261 tgcggcgcga acgccttatc cggcccacaa aacccgcaaa ttcaattaat ttcgcccctt
    13321 cacaggcctg tcaagcgtag cgcatcaggc aatttttccc gctttataat cccttcagca
    13381 acttttcaac aaactctggc accacctggc ttgccggacc gtaatatttc tcggcaaatt
    13441 cattaccaac ctgactcggt tcaagattca gttccacggt gtgcgcgcca tgcagtttcg
    13501 cttcgtgaac aaacccagcc gccggataaa catgccccga agtaccaata gcaatgaaaa
    13561 tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc
    13621 acactacgtg cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg
    13681 taacgtctcc ggtccagtcg aggacctggc cactttgcga acaacgtact ttcagcagtt
    13741 ccccatgcat atgaatcaca ttggtattgc ctgcgcgttc atgcagattg tcgatattct
    13801 gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttcgccagc gcaagatgtg
    13861 cagcgttagg ctgaatttct ggctgctgca actgtcgacg acgggcgtta taaaacgttt
    13921 gcaccagttc aggatcgcga tcaaaacctt ccggcgttgc cacatcttca acccgatgtt
    13981 cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaatcccag
    14041 cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga
    14101 aaaaaatacg ctgacgcaaa cgttcgcgca ggcgacgttt atttttacga aaacgactta
    14161 accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga
    14221 aggccgcacc acgcattccc cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac
    14281 gagctacagg taagagatga cgaggcaacc tttccgccag ttgcgttgtg attgccggga
    14341 aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc
    14401 ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc
    14461 cttgatcata aagcgcaatg atctcaggag cctgcaacgg ttgatgataa tagtgttgat
    14521 acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc
    14581 cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc ggcagacgca
    14641 tatggccaaa ctcgccggta atatagcttt taccggtaat tggtttaccg ttgaaaatca
    14701 acccgccgcc aacgccggtg ccgagaatca accccatcac cagtggatat tgagtaaatt
    14761 catcatccca tgcttcagaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc
    14821 gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca gggacattgg
    14881 cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac
    14941 ctttacagcc aaaacgttgg tcagcttcgg ctaccagctc gcacactgca tctaaaaatg
    15001 cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat
    15061 caaacacgcc tagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc
    15121 cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg
    15181 gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg
    15241 tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata gatatcgctg
    15301 gagaggaact gatgaccgat cagcttttca atccactcaa taatcggggt aagtttaagt
    15361 gaaaccacca cgccgataac cacaccacac aggctgccga acagccctgc cagcaatcca
    15421 taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg
    15481 atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc
    15541 acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga
    15601 tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt ggtcacttca
    15661 cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct
    15721 gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa
    15781 ctgtgatcga gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt
    15841 ggctgcatca gtttatgctc gggattcgag ttggggatca taatcgacac ccaatcgccc
    15901 tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct
    15961 ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc
    16021 tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgcgccact ttccaccagc
    16081 ccggtgaaat tgatatacgg cgcagcggcg gcaatgcccg gcactttctg cacgttatcc
    16141 agtgcttcct gccagttggt ccacggctga tctaccgcct caatttcacc gtgcggtact
    16201 accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg
    16261 atcaataccg ccacgccgag ggcaatgcca atggtagaaa tgacggagat caacgacacc
    16321 atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga taaaggcatc
    16381 gccattactc cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca
    16441 gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg aaggcggtgc
    16501 cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtcggcgtta cgtgcatcga
    16561 ggttaccagt aggttcatcc gccagtacca gccgcgggtt attgaccagc gcacgggcaa
    16621 tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct
    16681 ccagccccac cgcttttaac atctccagcg cacggctgtt gatttcagcg ggctttttct
    16741 tgccaatcaa cagcggcata gccacgtttt ccagggcggt aaaatccggc aacaggtggt
    16801 gaaactgata aataaatccc agcttctggt tacgcagttc cgctttggcc gccgacgaca
    16861 gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc
    16921 ctaacaggtg cagcaaggtg cttttaccgg aaccagagct gccgacgatc gccatcattt
    16981 caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca ctgccttcct
    17041 gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca
    17101 gcgggttgag tggcggcagc gcgccaggaa gggtaaagcg tagacagcag cgcgatagcc
    17161 atcgccacca gcgcaataac aatgacctgt aaaggctcga tagcgaccgg cagcgccgcg
    17221 ccatcaagca ggacgccgat tatcggcatc agattattca actggctggc aagcagtgcg
    17281 ccaagcgccg ctccgaggat cgcaccgata atcccagcgc tggccccttg caccataaag
    17341 accatcatga tttgacgcgg agtcagcccc tgagtctgaa gaatggccac ttcgccctgc
    17401 ttctccatca ccatcagtcc cagcgaggta ataatgttaa acgccgcaac ggcgacaatc
    17461 aggctcagca gcaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct
    17521 ttacggtcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaatgag
    17581 tcgactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc tgccggataa
    17641 cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg
    17701 ttggcggcga aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc
    17761 gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac
    17821 tgtgaggcaa gctgttcacc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt
    17881 ttcacattga ccagatacgg cgttagtgga tctttttgcg ccgggtcgat acccagcatc
    17941 accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg
    18001 acgcgattaa cgccgtccag tttgacggcc gtttccggga gttgctgcgg gttaagagag
    18061 ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg
    18121 cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg
    18181 ccgatggtag aaagccaaga gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg
    18241 taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtta
    18301 gcaaagacac gagtatataa gcgaaagcgg gatgggtaaa cgtctgaact gtaagtgatc
    18361 gtcagggcta atctgaaaag cccccgtaaa tcaatcggct aaagggcctc attttctcat
    18421 cggccttgtg ggctgatctt tatctgaaaa ataaccaaat acagacagat acgcattaca
    18481 ccacgcaact cgccccgcag gattaacagc atgttaaata aaaagagcgt gttgtcctga
    18541 tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga
    18601 tttatcactc ggtcattacc ttttatccgc atctgaccac tttccagcat ccgttatcag
    18661 aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt cgtatgccgg
    18721 tttttttctt tatctctggc tatttgattc gccgctatat cgacagcgta ccgtggggaa
    18781 attgtctcga taaacgcatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc
    18841 agtggctggc gctaagtgca ctaaatcagt ggctggcacc tgagcgcaat ttaagcaatg
    18901 cctccaatgc cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca
    18961 gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtgatatgt aaaattttta
    19021 accgcctggc cctgccgtta tttgttctgt ttgtactgat gagtgtggct gttaatttcg
    19081 tacccacacc gtggtgggga atgaacagtg tgagccgcaa tttgccttat tacagccttg
    19141 gcgcatggtt tggcgcaaca ttaatgacct gtgttaaagc ggtaccgttg cgccgccatc
    19201 tgctgatggc ttctttgctg gctgttctgg cggtcggtgc ctggttgttt aatatctcgc
    19261 tgctgttgtc gctggtatcg attgtggtga tcatgaagct gttttatcag tacgaacaac
    19321 gtttcggtat gcgctccacc agcctgctga atgtgattgg ttccaacacc attgctatct
    19381 acaccaccca tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg
    19441 cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc tttgttagtt
    19501 tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcgc gcattcagcg
    19561 atctgttgtt ctccccgcct tctctgcccg cggccgtcag ttactcccgc taagccatcg
    19621 ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg
    19681 cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt
    19741 atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc
    19801 gagttaactg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg tcacgccgga
    19861 ccagtggtgc ttattgcgcc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc
    19921 cagttcaccg atcagatggt gatgaacctg gcggactggg aaaccctccc ttacgacagt
    19981 ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg
    20041 atgcagcgtg gcgtactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccgcac
    20101 agttttctcc acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca
    20161 ttacgaaccc aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc
    20221 gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat
    20281 cgtcttgatt tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgatagccag
    20341 cgcacgctgg aggaagtaga agagatcaat ctgcttcccg cgcacgaatt tccgaccgat
    20401 aaagcggcaa ttgaactgtt ccgcagccag tggcgtgata ccttcgaagt gaagcgcgac
    20461 ccggaacata tttatcagca agtgagtaaa ggcacattac ctgccgggat cgagtactgg
    20521 cagccgctat tcttcagcga accgctgccg ccactgttca gttatttccc tgccaatacg
    20581 ctgttggtga atactggcga tctggaaaac agtgccgaac gtttccaggc tgacacgctg
    20641 gcgcgttttg agaatcgcgg cgtcgaccca atgcgcccac tgttgccacc acaatcgctc
    20701 tggctgcggg tggatgagct cttttcagag ctgaaaaact ggccgcgtgt gcagctaaaa
    20761 actgaacatt taccgacaaa agcagcgaat gccaatttag gtttccagaa actgccagac
    20821 ctggccattc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact
    20881 ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa
    20941 ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac
    21001 cgtgggcgtt atctgatgat tggcgctgcc gaacatggtt ttgtcgatac gatgcgtaac
    21061 ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg tcgtcaggat
    21121 tctcgccgcg ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt
    21181 cagccggtgg tccatctgga gcacggcgtc ggtcgttatg ccggaatgac cacgctcgaa
    21241 gctggaggca ttactggcga gtatttgatg ctcacctatg ccaacgacgc caaactgtat
    21301 gttccggtgt cgtcactgca tctgattagc cgttacgcgg gtggcgcaga agaaaacgca
    21361 ccactgcata aacttggtgg cgatgcctgg tcacgcgcac ggcagaaagc ggcggaaaaa
    21421 gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc
    21481 ttcgcgttta aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa
    21541 accacgccgg atcaggctca agccattaat gcggtgctta gcgacatgtg tcagccgctg
    21601 gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc
    21661 gccgctttcc tggcggtaga taaccacaaa caggtggcgg tgctggtgcc taccaccctt
    21721 ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc
    21781 gaaatgatct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg
    21841 gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt
    21901 aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag
    21961 cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttaccgcaac gccgatccca
    22021 cgtacgctga atatggcaat gagcggaatg cgcgatctgt cgattatcgc cacgccgccc
    22081 gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagtctggt ggtccgggag
    22141 gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa
    22201 aacatccaga aagccgccga acggctggca gaactggtac ctgaagcgcg gattgccatc
    22261 ggtcacgggc aaatgcgtga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa
    22321 cgtttcaacg tgctggtttg taccaccatt atcgaaaccg ggatcgacat tcctaccgcc
    22381 aacaccatta tcattgaacg cgcggatcac ttcggtctgg cacagttgca ccagttgcgc
    22441 ggtcgcgtcg ggcgttctca ccaccaggca tatgcatggc tactgacgcc gcatccaaaa
    22501 gcgatgacta ccgatgcgca aaaacgtctt gaagcgattg cctcgctgga agatctcggt
    22561 gcaggttttg cgctggcaac gcacgatctg gagatccgcg gcgcaggtga actgcttggc
    22621 gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa
    22681 aacgccgtcg atgcgctgaa agccggacgc gagccgtcgc tggaagatct caccagccag
    22741 caaacagaag tcgacctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg
    22801 aacacgcgtc tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa
    22861 gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc gcgtaccctg
    22921 ctggatattg cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt
    22981 aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc agcctggttg
    23041 attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgtctgaag
    23101 tttattcagg atttgagcga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt
    23161 gaactggaag agaacgcgat cgcttgatgg taaatcgggg gcgtctctgc gcccccatct
    23221 gcaatattta caaattcttt gcacttccct gcattatccg acactgtcac cctccataat
    23281 tcagacttac ttttttatta caataacctt atatttatta tggactttgg tgatgatcaa
    23341 aacgcgtttt tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct
    23401 accggcaaaa gccaacacct ggccgctgcc gtcagcggac agtcgtctgg ttggtgaaaa
    23461 caaatttcat gtggtggaaa atgacggtgg ttctctggaa gcgatcgcca aaaaatataa
    23521 cgtcggcttt ctcgctctgt tacaggctaa ccccggcgtt gatccttacg taccgcgcgc
    23581 gggtagcgtg ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat
    23641 tgtgatcaac attgcggagc tgcgtctcta ttattatccg ccgggaaaaa attcggtaac
    23701 cgtatatccc atcggcattg gtcagttagg tggtgacacg ctgacaccga caatggtgac
    23761 cactgtttct gacaagcgtg caaacccaac ctggacgcca acggcaaaca ttcgcgcgcg
    23821 gtataaggca cagggaattg agttgcctgc ggtagttccg gctggaccgg ataacccaat
    23881 gggccatcat gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa
    23941 cgctgatttc ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga
    24001 tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacacccc
    24061 gattaaagtc tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc
    24121 agagaagatt gatgacgatc cgcagctgct gccaattaca ctgaatagcg caatgcaatc
    24181 atttaaagat gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc
    24241 cgggatgccg gtggatgtcc gccgtcatca agtgagccca caaactctgt aaagctcgga
    24301 taaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa acaagcagta gcaggcatta
    24361 atgagggtta atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt
    24421 acagaagtaa tacggaaaga ttttgcgccc atctcatccg ctttttgcgc caactgctct
    24481 tccagcgatc ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct
    24541 tctggcgtgg actgaacttc gacagccgca aagctggcaa atgacatgga gcttaaaatc
    24601 gccgcagcga tgaggttttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg
    24661 tcgcaagcaa ttacttaacg at
//
LOCUS       DADTFG010000068        24199 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.068, whole genome shotgun sequence.
ACCESSION   DADTFG010000068 DADTFG010000000
VERSION     DADTFG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.068"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            11..1174
                     /gene="ompC"
                     /locus_tag="J1138_002521"
     CDS             11..1174
                     /gene="ompC"
                     /locus_tag="J1138_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBA8454300.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRVTALH
                     YFSDDKGDDGDKTYARLGFKGETQINDQLTGFGHWEYQFSGNKAESEGTAGNKTRLAF
                     AGLKFADLGSIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKSNGDGYGFSASYEFDGFGFVAAYTKSDRTGKQ
                     VNGLNGSKTIEVKDPLTGDVTEKEVSVDSGSVAKGKYAEMWGTGLKYDANNLYLAAIY
                     SETQNMTTFGDTGVADKAQNLEMVAQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQD
                     LVKYIDVGATYYFNKNMSTYVDYKINLIDESEFTQKAGIATDNIVAVGMTYQF"
     gene            1380..2078
                     /locus_tag="J1138_002522"
     CDS             1380..2078
                     /locus_tag="J1138_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA8454301.1"
                     /translation="MQDIHEESLNEAVKSAQSARVVLWEIDLTAQGGERYFFCGELNE
                     KGEPVTWQGRQYQAYPIEGCGFEMNGKGSSARPSLIVSNLFGLVTGMAEDLQSLVGGT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSALTCEKATFILATPAETDG
                     ALFPGRIMLANTCPWDYRGEECGYNGPAVADEFDKPTTDIRKDKCSKCLRGCEMRGNV
                     ANGGFFLSINKLSQ"
     gene            2084..2827
                     /locus_tag="J1138_002523"
     CDS             2084..2827
                     /locus_tag="J1138_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8454302.1"
                     /translation="MEQTESAILAHARRCAPAESCGFVVRTPEGEQYQPCVNISAEPE
                     AYFRIAPEDWLRADMQGEIVALVHSHPGGLPWLSEADRRLQVKSAIPWWLVCRGEIHH
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFAREDDWWRNGQNLYLDNLAEN
                     GFYRVSLSGAQAGDILLCCFGSSVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSVSAFTGIYNDLVAASACM"
     gene            2725..3363
                     /locus_tag="J1138_002524"
     CDS             2725..3363
                     /locus_tag="J1138_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBA8454303.1"
                     /translation="MATTNAFCLASPPLVRICLHGDLQRFGRRISLYVNTAAEAIRAL
                     SLQVPGFRQKLYEGWYQLRIAGRDVSEQELHSRVCEPLNDGDVIHLVPRTEGAKNGGV
                     LQVVAGAVLAVVGYVFSWTGIGAVIGNLGVAMMVGGITQMLTPRAKTPSATTTDNGKR
                     NTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGQVVVIGRG"
     gene            3607..7083
                     /locus_tag="J1138_002525"
     CDS             3607..7083
                     /locus_tag="J1138_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA8454304.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDMDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERSGHWVTEKDITINGKTT
                     SQYLTSVIIGNLPERPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQSYPNTAIV
                     GLQVDAEQFGGQQMAVNYHIRGRIIQVPSNYDPGKRTYSGIWDGTLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYALGQYCDQPVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWTYTAGNVVTGEDGVSFHYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFSVGAEGLRHTPGDIIEISDNAYAGTVIGGRILSVDNASRTLTLD
                     REVTLPEKGTSTVNLINGSGKPVSVDVTGQPSPDRIQISTLPDGVVEHSIWGLALPGL
                     RRRLFRCVAIRENADGTFAITAVQHVPEKEAIVDNGATFEPLSGSLNSVTPPAVQHLT
                     VEVSASDGQYLAVAKWDTPRVVKGVRFSLRLTSGTGENSRLVTSAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYFEIMVVPKLAVYDPTV
                     QFEFWFSEQQITDLLQVEKSARYLGTGSQWSVTGSRIKPGQDFWFYVRSVNLVGKSAF
                     VEASGHASNDAAGYLEMFREKIGKLHLAQELWSQIDNGQLKDEMAEMKTTITETRNEI
                     TQTVNKKLEDQSATIQQIQRVQKETDDSLSALYMLKVQKTKNGIPYVAGIGAGIEDVD
                     GQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNINANSGTLNNVTINQNCRILGKLSANQIEGDLVKTVGKA
                     FPRNNSYASGTITVTVYDDQGFDRQIIIPPVLFRGARHEHQSSKDHTSTWYSTCQLQV
                     QMNGRVIFHEPATDVTRVFSSVIDMPAGRGHVTLTFNVWSNGVNHWTPTTYISDLLVV
                     VMKKSTAGISIS"
     gene            7281..7421
                     /locus_tag="J1138_002526"
     CDS             7281..7421
                     /locus_tag="J1138_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBA8454305.1"
                     /translation="MSQKPLKTTVICITVVLIIWTTHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            7563..9023
                     /locus_tag="J1138_002527"
     CDS             7563..9023
                     /locus_tag="J1138_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8454306.1"
                     /translation="MAVRISGVLKDGAGKPVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEAGLYRVTLNTEGRMPSYVGDILVKADSAPGTLNKFLMDFEDAPYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDPGPQGVQGPKGETGATG
                     PKGERGETGAQGAPGPKGDTGATGPEGKAGPVGPKGDRGDTGLRGERGEKGEKGDPGG
                     PPGPKGDTGPAGPQGPKGDTGPQGPKGDTGPQGPQGKSGSLEDITDREAFIRELGVPR
                     AYGRDISTGAGEWTTEEFVAWLKEQGAFDHTFWMMKVALPAAMNKVITDTGLGKINLG
                     GCAVEVAGTYNAAIVRVTIGEYAATGFLNGTVCTCTVYGDTRKYHWRVDYSTRNKPTT
                     ASLAVNGWERDEATGRIRQWGRIDVSQADGKALSLHSVRFPVAFPSAVLNVQVSAVGD
                     QHTIQMYTLSEPSLQTVTLRTIKGSYGTFFWEATGY"
     gene            9033..9257
                     /locus_tag="J1138_002528"
     CDS             9033..9257
                     /locus_tag="J1138_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8454307.1"
                     /translation="MESYVFSPSANMFYPVSLRAVYEAAGNWPVDGIVVDYAVYKVFA
                     ADAAPAGMKRGVGTEKMPVWVPVSEEGTGK"
     gene            9254..9928
                     /locus_tag="J1138_002529"
     CDS             9254..9928
                     /locus_tag="J1138_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBA8454308.1"
                     /translation="MMHVRNFSYYTPAEPDVAGAIYLKSEDGQDWYECQSRFAEDTLK
                     VVYDSRGVITGYGKDIKLLWPVNQSVAEVPDTPESLKIDLSGRWGFDGEKITDLLTAD
                     RAREQKGAEINAWRSAMENAEYVFEYNGRKWDYGKVTQVRLEPSVAAAKAGILPEGFF
                     WTDAENNDVPMTAEALIALSAAAEKAMFQKGMEIHIRQRTMKKELEKLTSADEILAYP
                     VGWEMK"
     gene            complement(10091..10404)
                     /locus_tag="J1138_002530"
                     /pseudo
     CDS             complement(10091..10404)
                     /locus_tag="J1138_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007794049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(10569..11432)
                     /gene="potB"
                     /locus_tag="J1138_002531"
     CDS             complement(10569..11432)
                     /gene="potB"
                     /locus_tag="J1138_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBA8454309.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
     gene            complement(11416..12552)
                     /gene="potA"
                     /locus_tag="J1138_002532"
     CDS             complement(11416..12552)
                     /gene="potA"
                     /locus_tag="J1138_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBA8454310.1"
                     /translation="MGQSKKLNKQPNSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12802..14028
                     /gene="pepT"
                     /locus_tag="J1138_002533"
     CDS             12802..14028
                     /gene="pepT"
                     /locus_tag="J1138_002533"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBA8454311.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(14077..15198)
                     /locus_tag="J1138_002534"
     CDS             complement(14077..15198)
                     /locus_tag="J1138_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBA8454312.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(15274..16734)
                     /gene="phoQ"
                     /locus_tag="J1138_002535"
     CDS             complement(15274..16734)
                     /gene="phoQ"
                     /locus_tag="J1138_002535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBA8454313.1"
                     /translation="MKKLLHLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(16734..17405)
                     /gene="phoP"
                     /locus_tag="J1138_002536"
     CDS             complement(16734..17405)
                     /gene="phoP"
                     /locus_tag="J1138_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBA8454314.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(17575..18945)
                     /gene="purB"
                     /locus_tag="J1138_002537"
     CDS             complement(17575..18945)
                     /gene="purB"
                     /locus_tag="J1138_002537"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBA8454315.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(18949..19590)
                     /gene="hflD"
                     /locus_tag="J1138_002538"
     CDS             complement(18949..19590)
                     /gene="hflD"
                     /locus_tag="J1138_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBA8454316.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(19626..20732)
                     /gene="mnmA"
                     /locus_tag="J1138_002539"
     CDS             complement(19626..20732)
                     /gene="mnmA"
                     /locus_tag="J1138_002539"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBA8454317.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(20786..21247)
                     /gene="nudJ"
                     /locus_tag="J1138_002540"
     CDS             complement(20786..21247)
                     /gene="nudJ"
                     /locus_tag="J1138_002540"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBA8454318.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(21257..21910)
                     /gene="rluE"
                     /locus_tag="J1138_002541"
     CDS             complement(21257..21910)
                     /gene="rluE"
                     /locus_tag="J1138_002541"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBA8454319.1"
                     /translation="MRQFIISENTMQKTSFRNHKVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGNYSLDNLANGEWRDATD"
     gene            22082..23332
                     /gene="icd"
                     /locus_tag="J1138_002542"
     CDS             22082..23332
                     /gene="icd"
                     /locus_tag="J1138_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBA8454320.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(23446..>24199)
                     /locus_tag="J1138_002543"
     CDS             complement(23446..>24199)
                     /locus_tag="J1138_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8454321.1"
                     /translation="KDITALDIAEIIDAVKAEGHNRMAQVVRMVLIDVFKEAQHAGHV
                     PPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCGMLLALVTGQRLGDI
                     CNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVISQCRDAVVSKYLVHY
                     RHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQRSLSERLYREQGLD
                     TQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
BASE COUNT         5962 a         5798 c         6720 g         5719 t
ORIGIN      
        1 gattaaataa atgaaaaaaa tgacagtggc actttctgct gttgcagtgg cagtgatgtt
       61 tgctgcgggg gctcaggcag cagaagttta taataaagat ggtaataagc tggatcttta
      121 tggtcgtgta actgctctcc attacttctc agacgataaa ggtgatgatg gcgataagac
      181 ctatgcacgt cttggtttta aaggtgagac tcagatcaat gatcaactga caggttttgg
      241 tcactgggaa tatcagtttt caggtaataa ggcagagtct gaagggacgg caggcaataa
      301 aacccgtctg gcatttgccg gtttgaaatt cgctgattta ggtagcattg attacgggcg
      361 taattatggt atcgcttatg atgtcggttc atatacagac gttctgccag aatttggtgg
      421 cgatggctgg acgcagactg ataacttcat gactgctcgt acttccggag tgctgactta
      481 ccgcaataca gatttcttcg gactggtgga tggtcttaac tttgcagccc agtatcaggg
      541 aaaaaatgag cgagatgacc tccagaagtc taatggtgat ggatatggtt tctctgccag
      601 ctatgagttt gatggttttg gttttgtggc agcgtatacc aagtcagatc gtacaggtaa
      661 gcaggttaat ggcctgaatg gtagcaaaac tattgaagtc aaagatcctt taacggggga
      721 tgtgacggaa aaagaagtgt cagttgattc cggcagcgtt gcaaaaggta agtatgctga
      781 gatgtggggg actggcctta aatatgatgc aaacaatctg tatctggctg caatttattc
      841 cgaaacccag aatatgacca cttttggtga taccggggtt gcggataaag cacaaaacct
      901 tgaaatggtt gctcagtatc agttcgattt tggcctgcgt ccatctctgg cttatctgca
      961 gtcccgcgga caggatgtta tggttggtgg tgtgaaccat ggcgaccagg atctggttaa
     1021 atatattgat gtcggtgcga cttattactt taacaaaaat atgtccactt atgttgatta
     1081 taaaattaac ctgattgatg aaagcgaatt tacccagaaa gccggtattg cgacagataa
     1141 tatcgttgct gttgggatga cttatcagtt ctgattattg ctgataagtt aataaataca
     1201 gaccgtctgc ccttacaggc ggtctgtatc aatgaaaaca tagttttcat tggtcactgc
     1261 atcagcaatt gccatgcata tgctgattta attttctgtt attaccttta ttggttttat
     1321 ttaaaactga acgtattgtt cggggcgcgt ctgcgcccct tttttatggg cggatacata
     1381 tgcaggatat tcatgaggag agcctgaacg aggcagtaaa atcagcgcag tccgcccgcg
     1441 tggtgctgtg ggaaatcgac ctgacggcgc aggggggcga acgctatttt ttctgcggtg
     1501 aactcaatga aaagggcgag cctgtcacct ggcaggggcg tcagtatcag gcatacccga
     1561 tagagggctg cggctttgag atgaacggca agggcagcag tgcgcgaccg tcgctgattg
     1621 tctcgaatct gttcggactg gtcaccggca tggcggagga tttgcagagt ctggtcgggg
     1681 gcacggtggt tcgccgccgg gtgtacgccc gttttctcga tgccgtgaat tttgttgcgg
     1741 gaaacccgga ggcagaccct gagcaggagt tgacagatcg ctgggtggtg gagcagttgt
     1801 cagcgctgac ctgcgaaaag gcgaccttta tcctggcgac accggcagag acggacgggg
     1861 cgctgtttcc cggtcgtatc atgctggcca atacctgtcc gtgggattac cgtggagagg
     1921 aatgtgggta taacggcccg gcagtggctg acgagttcga caaacccacc acggatatca
     1981 ggaaagacaa atgcagcaag tgcctgcgcg gttgtgaaat gcgcgggaat gtggcaaacg
     2041 gcggtttttt cctttccatc aataaacttt cacagtgatt atcatggaac agactgaatc
     2101 agccattctg gcgcatgcac ggcggtgtgc gccagcggag tcgtgcggct tcgtggtgag
     2161 aacgccggag ggggagcagt atcagccgtg tgtgaatatt tctgcagagc cggaggcgta
     2221 ttttcgtatt gcgccggaag actggctgcg ggcggatatg cagggggaga ttgtggcgct
     2281 ggtccacagt catcccggcg gtctgccctg gctgagcgag gcggaccggc ggctgcaggt
     2341 aaaaagtgca attccctggt ggctggtctg ccggggcgaa attcatcatt tccgctgcgt
     2401 tccgcacctc accggacggc gttttgaaca cggtgtgacg gactgttaca ccctgttccg
     2461 ggatgcatac catctggcgg ggataacgct gccggatttt gcgcgtgagg atgactggtg
     2521 gcgcaacggt cagaacctgt atctggacaa tctggcggaa aacggctttt accgggtatc
     2581 cctgtccggt gcacaggcgg gagatattct gctgtgctgt tttggttcat cggtacccaa
     2641 tcatgctgcc atttactgtg gcaatggtga gctgcttcac catatacctg aacaactgag
     2701 taaacgggag aggtattctg aaaaatggca acgacgaacg cattctgtct ggcgtcaccg
     2761 ccactggtcc gtatctgcct tcacggggat ttacaacgat ttggtcgccg catcagcctg
     2821 tatgtgaaca cggcagcgga ggccatccgt gccctgtcgc tgcaggtgcc gggattccgg
     2881 cagaaactgt atgagggatg gtatcagtta cgcattgcgg gcagggatgt atctgaacag
     2941 gagcttcatt ccagagtatg tgagccgttg aatgacgggg atgttatcca tctggtgccc
     3001 cggacggagg gggcaaaaaa cggcggtgtt cttcaggtgg tggccggtgc tgttctggct
     3061 gttgtgggat atgtgttcag ctggaccggt attggtgctg tcatcggtaa tcttggggtg
     3121 gccatgatgg tggggggtat cacgcagatg ctcaccccca gggcgaaaac accgtctgcc
     3181 acgacaacag ataatggtaa acggaacacg tatttctcct cactggacaa catgattgcc
     3241 cagggtaacc cgatgccggt gccttatggt gagatgcttg tggggtcgcg gcggatatcc
     3301 caggacatca gcacccgcga tgagggcggg gacgggcagg tggtggttat cggtcggggg
     3361 tgagaataaa cataaaaaat acccacagta ttggccagac tgtggggaaa acgatgaaga
     3421 tgaactttaa ggagttattt tttgcagaag aacagtaacg cagcgtaatt atggtggcta
     3481 caggtaattg ccggaagtgt gaagaaattc agaaatttta ttcactcagc agacaggcac
     3541 cctccggggt gcctgttgtt tttgtacata aacagagact gacatcagac actaaagggt
     3601 gacgcaatgg gtaagggggg cggcaagggg cacacaccgc gtgaggcaaa ggataacctg
     3661 aagtccacgc agatgatgag cgtgattgat gccattggtg aagggccggt tgaaggtccg
     3721 gtgaagggac tgcagagtat tctggtgaac aaaaccccgc tgacggatat ggacggtaat
     3781 cccgtgatac atggcgtgac agccgtctgg cgtgccgggg agcaggagca gacgccgccg
     3841 gaaggctttg agtcttccgg tgcggaaacc gcactgggcg tggaggtgac gagggcgaag
     3901 ccggtgacgc gcaccatcac gtcagcgaac attgaccgtc tgcgggtcac cttcggggtt
     3961 cagtcactgg tggagacgac ctcaaagggt gaccgtaatc cctcttctgt ccggttgctg
     4021 attcagcttg agcgtagcgg gcactgggtg acagagaagg atatcaccat taacggtaag
     4081 accacctcgc agtacctgac gtcggtgata atcggtaacc tccctgagcg tccctttaat
     4141 atccggatgg tcagagagac ggcggacagt accacagacc agctgcagaa cagaacgctc
     4201 tggtcgtcat acaccgaaat catcgatgtg aaacagagct acccgaacac agccatagtg
     4261 gggctgcagg tggatgcgga gcagttcggt gggcagcaga tggcggtgaa ctaccatatc
     4321 cgcgggcgca tcatccaggt gccgtcaaac tatgacccgg gaaaacgtac atacagcggt
     4381 atctgggacg ggactctgaa accggcatac agcaacaacc cggcatggtg tctgtgggat
     4441 atgctgacgc atccacgtta tggtatggga aagcgcctcg gggccgcgga tgtggacaag
     4501 tgggcgctct atgccctcgg acagtactgt gaccagccgg ttccggatgg ctttggtggt
     4561 acagaacccc gtatgacctt taatgcgtac ctgtcacaac agcgtaaggc gtgggacgtg
     4621 ctgggggatt tctgctctgc aatgcgctgt atgccggtat ggaacggaca gacgctgacg
     4681 tttgttcagg acagaccatc ggatgttgtc tggacttata ccgccggtaa tgtggtgacg
     4741 ggggaggatg gggtaagttt tcattacagc ttcagtgccc tcaaggacag acacacggca
     4801 gttgaggtga attacaccga tccgcagaat ggctggcaga catcgactga actggtggag
     4861 gatccggatg cgatactgcg ttacggacgc aatcttctga agatggatgc gttcggctgt
     4921 accagccgtg gtcaggctca ccgggcaggc ctgtgggtga taaagaccga actgctggaa
     4981 acacagacag tggatttcag tgtgggggcg gagggactgc gtcacacgcc cggcgatatc
     5041 attgaaatca gtgataatgc gtatgccggt accgttatcg gcgggcggat cctgtccgtc
     5101 gacaacgcca gccggaccct gacgcttgac cgtgaagtca ccctcccgga aaagggcaca
     5161 tccacggtga atctgataaa cggcagtggt aagccggtga gtgtggatgt taccgggcag
     5221 ccatcacctg acaggataca gatcagcaca ctgcctgacg gggtggtgga acacagtatc
     5281 tgggggctgg cattgccggg actgcgccgc cgtctgttcc ggtgtgtggc gatccgcgaa
     5341 aatgcggacg gtaccttcgc catcacggcg gtgcagcatg tgccggagaa ggaggccatc
     5401 gtggataatg gtgccacctt tgagccgttg tccggttcgc tgaacagtgt cacacctccg
     5461 gcagtacagc atctcacggt ggaggtaagc gcgtctgacg gtcagtatct ggcggtggca
     5521 aaatgggaca cgccgagggt ggtgaagggc gtgcgcttca gtctgcgcct gaccagcgga
     5581 accggtgaaa acagccgtct ggtgaccagc gccatcaccg ccgacacgga gcaccgtttc
     5641 agcggtctgc ctctggggga gtacaccctg acggtcaggg cgataaacag ctatggccag
     5701 cagggggagc ctgccaccac cacgttccgg attaacgcac cggcagcacc tgccagcatt
     5761 gaactgacgc cgggttattt tgaaataatg gtggtaccga aactggctgt atatgatccg
     5821 accgttcagt ttgagttctg gttttcggaa cagcagatta cagatctgtt acaggtggag
     5881 aaatctgccc gttatctggg gacaggcagt cagtggagtg ttaccggttc ccgtattaaa
     5941 ccgggacagg atttctggtt ttacgtacgc agcgttaacc tggtcggtaa atccgcgttt
     6001 gtggaagcta gcgggcatgc cagtaatgat gccgccggat atctggagat gttccgggaa
     6061 aagataggaa aactgcatct ggcacaggaa ttgtggtcgc agattgacaa cggccagttg
     6121 aaggatgaga tggcggaaat gaagaccacc atcacggaaa cccggaacga aatcacacag
     6181 acggtcaata aaaagctgga agaccagagt gccaccatac agcagataca gcgtgtgcag
     6241 aaggagacgg atgacagcct gagcgcactg tacatgctga aggtgcagaa aacgaaaaac
     6301 ggtattccgt atgttgccgg tattggtgca gggattgagg atgttgatgg ccagccactg
     6361 agcaatattc tgctgcaggc ggaccgtatc gcgatgatta acccggagaa cggcaacacc
     6421 acgccgctgt ttgtggcgca ggggaatcag ttgttcatga acgacgtgtt cctgaagcga
     6481 ctgtttgcgg tgagcatcac ctcgtccggc aatcccccga cgttctccct gacgccggaa
     6541 ggcaggctga cggcccgtaa tgcggatatc agcggaaata ttaatgcgaa ctctggcacg
     6601 ctcaataatg tgaccattaa ccagaactgc cggattctgg gaaaactgtc tgcaaaccag
     6661 attgaaggtg acctggtcaa aacagtgggg aaagctttcc cacggaataa cagttatgcc
     6721 agtggcacga taacggtcac cgtttatgat gaccagggat tcgacaggca gatcatcatt
     6781 ccgccggtac tgttccgggg ggcgagacat gaacaccagt ccagcaaaga tcacacttca
     6841 acatggtatt ccacgtgtca gttacaggtg cagatgaacg gaagggtgat ttttcatgag
     6901 ccggcaacgg atgtcacccg ggtgttttcg tccgtcattg atatgccggc aggacggggt
     6961 catgtgaccc tgacgtttaa tgtgtggtcg aacggggtga accactggac gccgacaaca
     7021 tacatcagtg atttactggt tgtggtcatg aaaaaatcca cggcgggtat cagtatttcg
     7081 tgaacatgat gaccggaatt gccggaagga gataaaaacc gtacagtatg cgcgggtgcc
     7141 tttggctgat ggccggaggg aacacctgaa ggccggatgt gaaaaggccc cggacaaaac
     7201 attcatgttt aacccgaggc ctgaccattc atcctaagca agtgaaaggt tagcgcctct
     7261 ccgtaaaagg agcaagcgtt atgtcgcaaa aaccgttaaa aaccaccgtg atttgtatca
     7321 cggtagtgct cattatctgg accacccaca gttcactgtg tgaattccgg ttccggatag
     7381 cgggcgcgga gattgcggcg ttcttacagt gtaaacagta agaaaccgtg gcgggggacc
     7441 ctgtcccccg ctgaccggtt gctgaaggtg gtcagccgga tggcaccttt ttaatcctgt
     7501 aaaccacgat ttttaccgca ggccgggaaa ccggcactgc ggttttttta tggggaaaat
     7561 ctatggcagt cagaatatct ggtgtgctga aggacggcgc agggaagccg gtaccgggat
     7621 gcacgataga gctgaaagcg cgacgtacca ccgaaacggt gattgtcacc acggtggcat
     7681 acgggcagcc gggggagaca ggcagctaca gtatggatgt tgaagcgggg ctgtaccgtg
     7741 tgaccctgaa cacagaaggg cgtatgccgt catacgtcgg tgacattctg gtgaaggcgg
     7801 actctgcacc gggaacactg aataaattcc tgatggactt tgaggatgcg ccatattatc
     7861 cgaaagccct cgctgagctg gaagcggtgg cagcggaaat actgaagcgt gctgaagcct
     7921 ctgcagccag tgcagaggag gccaaaaaac gggcagagaa tgccagaggg ccgaagggtg
     7981 ataaaggcga cccgggaccg cagggggtac agggaccgaa aggtgagaca ggtgcaaccg
     8041 ggccaaaggg ggagcgtggt gaaacaggtg cacagggtgc tccggggcca aaaggggata
     8101 ccggtgccac aggacccgag ggtaaagccg gtcctgtggg accaaagggt gaccgggggg
     8161 atacggggct cagaggtgag cggggcgaga agggagagaa aggagacccg ggtggccctc
     8221 ccgggccaaa gggcgataca ggcccggcgg ggccgcaggg accaaaagga gataccggtc
     8281 cacagggacc aaagggggat accggaccgc aggggcctca gggaaagtca ggcagccttg
     8341 aagatattac agacagggaa gccttcatcc gtgagcttgg tgtgcccaga gcatacgggc
     8401 gtgatatttc aaccggcgca ggtgagtgga cgacagaaga gtttgttgcc tggctgaaag
     8461 agcagggggc atttgaccac accttctgga tgatgaaggt ggcactgcct gcggcgatga
     8521 ataaggttat tacagatacg ggactcggga aaattaacct gggcggatgt gctgtagagg
     8581 tggcaggaac atataacgct gcgattgtcc gtgtgaccat cggggaatac gccgcaacgg
     8641 ggtttctcaa cggaacggtc tgcacctgta ctgtttatgg cgatacccgg aaataccact
     8701 ggcgggtgga ttacagtacc aggaataaac ccacgacggc gagtctggct gtcaatggct
     8761 gggaacgtga tgaggccaca ggccggatac ggcaatgggg gcgtattgac gtgagtcagg
     8821 ctgatggtaa agcactgtcc ctgcactccg ttcgtttccc tgttgctttt ccgagtgctg
     8881 tactgaatgt tcaggtatct gcagtgggag accagcatac cattcagatg tataccctgt
     8941 cagaaccgtc attacagacg gtgacacttc ggactataaa ggggagttac gggacatttt
     9001 tctgggaagc gaccggatac tgaggaggga ttatggagag ttatgttttt tcaccgtccg
     9061 cgaatatgtt ttatccggta tccctgcgtg ccgtttatga ggccgccggg aactggcctg
     9121 tggatggtat cgtggtggac tatgccgtgt ataaagtttt tgcagcggat gctgcaccgg
     9181 cgggcatgaa gcggggcgtc ggtacggaaa aaatgccggt ctgggtgccg gtatcggaag
     9241 agggcacggg aaaatgatgc atgtcagaaa tttttcgtat tacacaccgg cagagccgga
     9301 tgtggcgggt gcgatatacc tgaaatctga ggatggtcag gactggtatg aatgccagtc
     9361 caggtttgcg gaagacacgc tgaaggtggt gtatgacagc cgtggtgtga ttacgggata
     9421 tggtaaggac attaagttgt tgtggccggt gaaccagagt gtggctgaag tgccggacac
     9481 accggagagc ctgaaaattg acctgagcgg acgctggggg tttgacgggg aaaaaatcac
     9541 cgacctgctg acggctgaca gagcccgtga acagaagggg gcagaaataa atgcctggcg
     9601 aagtgccatg gaaaatgcgg agtatgtgtt tgagtacaat ggacggaagt gggactacgg
     9661 caaggtgact caggttcgtc ttgagccgtc ggtggcagca gcaaaagcgg gaatactgcc
     9721 ggagggattt ttctggacag atgcagaaaa taatgacgtg ccgatgacag cggaggcttt
     9781 gatcgcgctc agtgctgcgg cagaaaaggc gatgtttcag aaagggatgg aaatccacat
     9841 tcgccagcgc acgatgaaga aagagctgga aaagctgact agtgcggatg aaatactggc
     9901 atatccggtt ggctgggaga tgaaataata aaaaaagggg ggagaaacca tcccccataa
     9961 aagaaggtca taatgatgac ttagtgtaga tagttacgaa tgcaaccaga tactgccaca
    10021 ggaaattctg ctgtaactgt gatgctgctc gtattttcgc gggcatcagt atttttcaga
    10081 ttttcggaag tcaatatatt gctcgtgcgc ggttacatgc ctcattgatt tcgccatttt
    10141 cgccggacat tgcgaattcc ctgcatgtgg acggacggtt tttgtaaaca gagcaatatg
    10201 cgttttcacc ggggtgccgg ccagagcgac acatcgggga tttttctgat tggtgccgcg
    10261 catacagcga tgaaaggggg atgtttgctc agtgagactg gccgggacgt ttccgccaga
    10321 atcgtcagct tcagcccagt aaaaagagac acgaaaaaat gcacaacagg caccacacgt
    10381 catgtatgga ttaggattgc tcatttttat actatttgtt gttttttgca gcgattagat
    10441 aaaagggggc tgaaaaaaca ttctccaaaa ttagattata attgtttgat tttatggata
    10501 gatgcatgct ttttacgcaa gagtaaaaag catgccaagt ttgccattaa ctctttgaat
    10561 actcagcact aatcatctat ttcggttgcc ttcttattca gcagacgaga agcgcgccag
    10621 taaaccagca acatcaggcc cattacgata gtcagcgtaa tgctggtggc tgcaccaaac
    10681 ggccagtcac gaatattgag gaactgtacc ttgatgacgt taccgatcag caggtttttc
    10741 gcaccgccca tcaggtcgga cacatagaac aggcccatcg ctggcagcat caccagcaga
    10801 catccggcaa taattcccgg catcgttagt gggataatga tgcggataaa ggtctgcaac
    10861 ttgctggcac cgagatcgcg cgccgcctca agcaacggct tatccagttt ttcgatactg
    10921 gagtacagcg gcatcaccat aaacggcagc agaatgtaaa ccagaccgat aatcaccgca
    10981 ctaggcgtga acatgatgcg aattggtgta tcgataacgc ccagccagag caaaaactcg
    11041 ttgagatagc ctttggtgct gaggaaaatt ttcagcccgt aaatacgaat caatgagttg
    11101 gtccagaacg gaacaatcag cagaaacagc agcagcggac gcaccttgtg tggcagcttc
    11161 gccagaaacc aggcaaaagg atagcccagc accaggcagg cgagggtggc gatcagcgcc
    11221 atattcagcg agtgcaatag cacttcaaaa tagagcggat cgagcagacg cgtgtagtta
    11281 tccagcgtaa agaccatttt gacgaaactg gcgtcgtcgc gggtcaaaaa gctggtgcca
    11341 atgatcatca ggttgggcag aaagacaaac aacacaagcc aaccgacaat agtgacaatc
    11401 actacattct ggaacttact tgtgttcttc atcagccagt acgacctccc agctttctac
    11461 ccaattaatg gccatttttt ggtcgagaga gtggtcaaag tcaggatcgt cttcattgaa
    11521 gaattcgctg accatcacca tcttgccatt ttccagctca acaaccgact ccagcgtcat
    11581 gcctttgtag ttacgctcgc gaacgtaacc aatcagccct tcggcgtggt tgtcgtcgtt
    11641 aatctcttca acacgtaagt cttccgggcg cagcagaaca tgcagttttt gccccggttc
    11701 aacggcgaag ttaacgtaga tattacattc gcggccttca acgttggcgc gtacgcgctg
    11761 ctcgtcgaga cgttcgatga cagtggcgtt aaacatattg atttcgccaa taaacccggc
    11821 aacaaacagg tttttcggct cttcgtagat ttcacgcggc gtgccgtctt gctcaatgcg
    11881 accatcgcgc atcaccacga tcctgtccga catggtaagt gcttcttcct ggtcgtgagt
    11941 tacgaacacg aatgtaatgc caagcttacg ctgtaacgct ttcagctcgt tctgcatttg
    12001 cttgcgcagt ttgtaatcca gcgctgagag cgactcatcc agcaacaaca gacgaggctt
    12061 gttaaccacc gcgcgagcaa tggcgacgcg ttgctgttga ccaccagaga gctgatgcgg
    12121 tttgcgttga gcgaaggttt ccagctgcac catccgcagg gcttccatca cgcggggcgt
    12181 aatttcagca gcgggggttt tttgcatgcg caacccaaag gccacatttt cgaacacggt
    12241 catgtggggg aaaagtgcgt agctttggaa aacagtgttc acatagcggt tttccgccgg
    12301 aacgtgggtg atgtcctcgt tatccagcat gatgcgtccg gaatcaacag tttccagacc
    12361 tgcaatcagg cgaagaacgg ttgttttacc gcagccagaa gggccaagca gcgtgaggaa
    12421 ctcgccattg ttgatagtca gatccagctg gggaatgacc tctttaccat caaagcattt
    12481 gcgaattccc gccaattgca ccagcggtga aagcgaattc ggttgtttat tcaatttttt
    12541 actctgtccc atgtaaacgc aacggatggc ttaccgatgc ggggtttgtg gttaaccacc
    12601 ttggtgactc ttaatgaggg cggtaattct acggcaaacc gcttgaatcg ccaatctttg
    12661 ttgtgaatta ctggcttagc tttatattca ttaaggtaat gctgataaat attcccgctt
    12721 gcagggctaa aagtgacctg acgcaatatt tgtcttttct tgcttcttaa taatgttgtc
    12781 acaaaaagtg agggtgacta catggataaa ctacttgagc gatttttgaa ctacgtgtct
    12841 ctggataccc aatcaaaggc aggggtgaga caggttccca gcacggaagg ccaatggaag
    12901 ttattgcatc tgctgaaaga gcagctcgaa gagatggggc ttatcaatgt gaccttaagt
    12961 gagaagggaa ctttgatggc gacgttaccg gctaacgtcc ctggcgatat cccggcgatt
    13021 ggctttattt ctcatgtgga tacctcaccg gattgcagcg gcaaaaatgt gaatccgcaa
    13081 attgttgaaa actatcgcgg tggcgatatt gcgctgggta tcggcgatga agttttatca
    13141 ccggttatgt tcccggtgct gcatcagcta ctgggtcaga cgctgattac caccgatggt
    13201 aaaaccttgt taggtgccga tgacaaagca ggtattgcag aaatcatgac cgcgctggcg
    13261 gtattacaac aaaaaaacat tccgcatggt gatattcgcg tcgcctttac cccggatgaa
    13321 gaagtgggca aaggggcgaa acattttgat gttgatgcct tcgatgcccg ctgggcttac
    13381 accgttgatg gtggtggcgt aggtgaactg gagtttgaaa acttcaacgc cgcgtcggtc
    13441 aatatcaaaa ttgtcggtaa caatgttcac ccgggcacgg cgaaaggagt gatggtaaat
    13501 gcgctgtcgc tggcggcacg tattcatgcg gaagttccgg cggatgaaag cccggaaatg
    13561 acagaaggct atgaaggttt ctatcacctg gcgagcatga aaggcaccgt tgaacgggcc
    13621 gatatgcact acatcatccg tgatttcgac cgtaaacagt ttgaagcgcg taaacgtaaa
    13681 atgatggaga tcgccaaaaa agtgggcaaa gggttacatc ctgattgcta cattgaactg
    13741 gtgattgaag acagttacta caatatgcgc gagaaagtgg ttgagcatcc gcatattctc
    13801 gatatcgccc agcaggcgat gcgtgactgc gatattgaac cggaactgaa accgatccgc
    13861 ggcggtaccg acggcgcgca gttgtcgttt atgggattac cgtgcccgaa cctgttcact
    13921 ggcggttaca actatcatgg taagcatgag tttgtgactc tggaaggtat ggaaaaagcg
    13981 gtgcaggtga tcgtccgtat tgccgagttg acggcgcaac ggaagtaagc gaaaagggat
    14041 gcggcatgtg atgccgcatc cggcttaaat ccaaacttac ccttcgaaga accagtatcc
    14101 gctattgacc agcgccgcga gcatcgcgag gaatgacgga tcttccagcg catcgccaaa
    14161 attctccgcg gtcagcgcaa tgttgctggc gagcgcatcc agtgccggac ggtgcgggga
    14221 atcgatcttc tcaccattgg catacacgtc gtcgccaatg cgcaatacgc gcagaccccc
    14281 caggcgcacc agcacatcac cttgtttcag cgcatcgtag atttcatccg gctgataagg
    14341 cggctctggc ggcgcgatat ccagttcatg acgtgactgg gatataaact cgccaaacca
    14401 ttgtttaaag tgttccggct ggttgatcaa ttcgagcatc atctcacgca gtttatccat
    14461 ctcttgcggc agaacgtccg caggatgagc gcgaggtgga acatcaggat cgctgtagta
    14521 gttgccgccc agttcacgtt gcagcacata atcggcaaat ccgctaatca gttcccgcgt
    14581 atttggcgcg cgaaaaccca cggaatagtt catcgcattt tccagcgcgt agccttcatg
    14641 cgggaatcct ggcggaatat aaagaatatc gccaggctcc agctcttcat cgatgatggc
    14701 ttcgaacgga tcgacctgta acaggtccgg gtgcgggcag tgctgtttca tttgcagctt
    14761 ttcgcccact cgccagcgac gacgaccggt accctgaatg ataaacacgt cgtactgatc
    14821 gagatgcggg ccgacgccgc cgcccggtac agaaaaagag atcatcagat catcaatacg
    14881 ccagtccggt agttcacgga acggtcgcat tagcgcggcg gtcggctcat gccagtggtt
    14941 cactgcttgc actaacaatg accagttagt ttcaccgaga tgatcgtagc tttcgaatgg
    15001 accgtggctg acctgccatt ttccatcctg atgactgacc agtcgactgt cgacttcgct
    15061 ttccatcgcc agacccgcca actcgtctgg agagattggg tcaataaaat tattaaagcc
    15121 gcgttttaac accaccgggc gtttctgcca gtgacgttca agaaaatcgg gccagttaag
    15181 agtgagttgg tattccatgt taagcatcca caggctggta tctgcaaccg attataacgg
    15241 atgcttaacg taatgcgtga agtatggaca tatttattca tctttcggcg cagaatgctg
    15301 gcgaccaaaa atcacttcca tccgcgcacc gcccagcatg ctctctccgg cgacgatttt
    15361 accctcatat tgctcggtga tttcgcgggc taccgccagc cccacacctt gcccagggcg
    15421 taaagtatca acccgttgac cacggtcgaa aatgacctct cgcttgctta atggaatacc
    15481 ggggccatca tcctcgacca caatatagag atgctcgtcg gtttgccttg cagaaatttc
    15541 gacaaactcg aggcaatatt tacaggcgtt atccagcacg ttgcccatca cttcgacaaa
    15601 atcattctgc tcaccaacaa agctgatttc tggcgagata tcgagagaga tattgacccc
    15661 tttgcgttga tacactttgt tcagcgccga ggtgagattg tccaacagtg gcgcgaccgg
    15721 atgcagctcg cggctaagca atgtcccgcc gcgcatactg gcacgatgca ggtagtagcc
    15781 aatttgctgt gaaatgcggc tgatttgctc cagcattacc ggctcagcat cactgacgct
    15841 catcttctca ctacgcagag aacgcagcgt actttgcagc accgccagtg gcgttttcag
    15901 actatgggtc aggtcggtga gcgttgtacg atatttgtcg taacgttcgc gttcactttt
    15961 taacagtcgg ttcaggtttc gtaccagact ggtcagttct cgcgttgtgg ctggattgag
    16021 caattcacgg ttatgttctt ccagttcgcg gacttctttt gccagggctt cgatggggcg
    16081 taaactccac caggcggcga cccacagcag cgggatcact aacagcagat tggctgagag
    16141 cacatagata aaccagctcc agaccatata ggaacttttt agctccaccg gaatggtatc
    16201 caccaccaca atggttaact ttggcatccg cgatgttgcc gggtagacgt ttaccgccac
    16261 cgagtgggtc atctccgcgt cgtcatcatc ttcccgcact tcctgcaact gttgctgtat
    16321 cgaatgatct ccactcagca agaggctggt atcgttaaca tccgcttcaa tttcatgaaa
    16381 accattcgat ttcagccagt caggctggat catcttcatc agccagggca cgtcacgttg
    16441 cgcccataaa agctgcccgt tctcatcata aattagtgtc atcgtggggc tttgcttgtc
    16501 gatattttcg ggtaactcga catgcaactt attgttttcc cacttcgcaa gggtatagaa
    16561 cagattgctc tcgccacgta acagccgaaa cgtggtttta tcgaaactga cgctataacc
    16621 gatcagcgca accattccgt aggcaagcga aagcaccaat actactgctg ccgttgctaa
    16681 cagaaaacgt acccgcagcg agagcgggaa aaaaagatgc agtaattttt tcatcagcgc
    16741 aattcgaaca gatagccctg gccgcgaacg gtggtaatca cttcttgcgg atattgtgcc
    16801 tgtatttttt tgcgcagacg tcccatcagt acatctatgg tatgactttc ccgcagctcc
    16861 gcatccgggt agagttgtag cattaacgaa tctttgctga ccactttgcc attattgcgt
    16921 atcaacgttt ccatgatggt gtattcgaac gcggtcagtt tgatcacttc gtcattaata
    16981 gataattcac ggcgggagag atcaacctga aacggtggta gcgaaatgac ctgtgaagcc
    17041 agaccgctat tacgccgcat taatgcctgc attcgcgcca tcacctcttc aatatgaaac
    17101 ggtttagtca cgtaatcatc agcaccggca cttaatactt cgactttgtc ctgccagctt
    17161 tcacgggcgg ttaataccag aatcggcagt gaaacatcat tgctacgcca gcggcgaatc
    17221 agtgacagac cgtcctcgtc aggcaaaccg agatcgacaa tcgcaatatc cggtaaatgt
    17281 tcattgagat aataatcggc ttctttggca tcttctgcat catcgacctg atgaccagca
    17341 tcctgaatct gaactttaag gtggtgacgt aacaacgcat tgtcttcaac aaccagtacg
    17401 cgcattttta tttctccctg tcttaattat taaaatagtg taacgcgatt atgtggttat
    17461 gggggtaaac attaaataaa ccagcggggg aggggaggta aagtgaaaaa ataaaaagcg
    17521 gataatctta ataagcaggc cggacagcat cgccatccgg cactgttttg aggcttattt
    17581 cagctcatca accatggtga tggcgcgacc aatgtagtta gccggcgtca tcgctttcag
    17641 gcgggctttc tcttcttctg gcaacgccag accatcgata aactgcttca tgccttcggc
    17701 gtcaacgcgc ttaccgcgag tcagctcttt cagcttctcg tacggttttt cgatgccata
    17761 gcgacgcata actgtctgga ttggttcagc cagcacttcc cagttgtgat ccagttcatc
    17821 cagtagatgg tcacggttca cttccagttt actcacgcct ttcagggtgg attgatacgc
    17881 aatcagcgca taaccgatac ccacgccgag gttacgcagc acggtggagt cggtcaggtc
    17941 acgctgccag cgggaaactg gcagtttgct tgccaggtgc tgcaataccg cgttggaaag
    18001 ccccaggttt ccttcggagt tttcgaagtc gatcgggtta actttatgcg gcatggtgga
    18061 agaaccaatc tcaccagcaa tggttttctg tttgaagtgg ttaagggcga tataacccca
    18121 gacgtcacgg tcaaagtcga tcagaatggt gttgaagcgc gcaacgcaat caaacagttc
    18181 ggcaatgtag tcatgcggtt cgatctgggt ggtgtacggg ttccactgaa tacccagcga
    18241 ggtgacgaac tcttcgctga actgatgcca gtcaacttcc gggtaagcgg cgatgtgggc
    18301 gttgtagtta ccgaccgcac cattgatttt gccgaggatc tccacctggt taagctggcg
    18361 gtactggcgc tccatacggt aggcgacgtt agccatctct ttaccgatgg ttgacggcgt
    18421 ggctggctga ccgtgggtac gagacagcag cgggatatcg cgatactgaa cggcgagatc
    18481 tttaatgcca tcaatcagtt gacgccagta cggcaggatc acttcatcac gcgcggtttt
    18541 cagcattaat gcgtgggaga ggttattgat atcttccgaa gtacaggcaa agtggatgaa
    18601 ttcagaaacc gcgtgcagtt ccgggatatc cgccactttt tctttcagga aatactcaac
    18661 cgctttaacg tcgtggttag tggtacgctc gatggttttg atgcgtgctg catcttcttc
    18721 gctgaaactg gcgacaattg catcaaggta accgattgcg tcggcagcaa aagcaggaac
    18781 ttccttgatc gctgcgtgcg cggccagttt ttgcagccaa cgtacttcaa cttgtacacg
    18841 gaatttcagc aaaccgtatt cgctgaaaat cccgcgcagc gcgctgactt tatcgccgta
    18901 gcgtccatcg acaggggaaa cggcggtcag tgaggataat tccatagatc acaactccgg
    18961 ggttaaatga gcaagaattt gttttgcctg agtggtcagg cgattacgag aaaacatcag
    19021 ttgcagacga ccgccgccga cctggtgcca gagcacggcg gcgcgaatgc ctgccagcag
    19081 tgttgcgcga actttcgcct gcacctgtgg gctttgcagt acagcagggg aaccagtgac
    19141 ctgaatgcgc gggccaagcg ggctgataac atcaacatag atggcagcca tcgcgctcat
    19201 cagcgtttcg gactgtaaat cgaagtgttc gagctggcgt tgcaggccgt tgatccggtt
    19261 gcccagagtg tcgagcgcac ctttcgctga ggagagtttg cgctcaagca ccatcagact
    19321 gagtgtgtag cgggttaatt cggcgtttaa gccctggcga ctgctggcat tgagtacgcc
    19381 gagcaaggtt tccagcccga cgcgcaggtt ggcttcgcta ccgccaaaaa ccgccagcgt
    19441 tgagctgggg ttcatatcga taatactgtt gagtgagacg tgtagcgcat cgccatcaca
    19501 atgcccctgg tgagcgagtt gttgcaccag gcgtgccgac tgacaaatac cggcaagggc
    19561 gagggtgatg tcatagtaat tctttgccac gttcactgct tccttgtttt aagtaaagat
    19621 aataatcaga ccggcagcgg cagacgctgc tcaataatac cgccaccgag gcacacttca
    19681 ccgttataga aaacggcaga ctggcccggc gtcacggcgg caaccggctc atcgaaaatc
    19741 acttcaatgc gatcagcgtc cagcgccttg acggtgcacg ggatgtcggt ctggcgatag
    19801 cgggttttta ctgtgcaacg catagtaccg gtgaacggct cgcgatcgac ccagtgcagc
    19861 tgctgggcaa tcagaccgac agacatcaga cgcgggtgtt catggccttg cgcaacaatc
    19921 agaatgttgt tttcgacgtc tttatcgacc acataccacg gttcttcggt gccgtctttg
    19981 gtgccaccga tacccagacc tttacgctga ccgagggtgt gatacatcag cccctggtgc
    20041 tcaccaattt catcgccatc gacggtaatg attttgcccg gttgcgccgg gagataacgg
    20101 cccaggaact cgcggaattt acgctcgccg ataaagcaga taccggtaga atcttttttc
    20161 ttcgcggtga ccagaccgag atcttcagca atcttacgca cctgcggttt ttccagttcg
    20221 ccgaccggga acaggctttg cgcaatttgc tcatggctga gcgtataaag gaagtagctc
    20281 tggtctttat tgctgtccag gccacgcagc aggcggctct tgccatcgac atctgcccga
    20341 cgtacgtaat gaccggtagc gatataatcg gcacctaaat cttcggcggc aaattcgagg
    20401 aaagctttaa atttgatctc tttgttgcac agaatatccg gatttggcgt gcgaccggct
    20461 ttatattcgg caaggaacag ttcgaagacg ttgtcccagt actcggcagc aaagttaacg
    20521 gtgtgcagtt caatgccgag cttgtcgcag acagcctggg catcagccag atccgctgcc
    20581 gctgtgcaat attcctcacc gtcgtcttct tcccagttct tcataaacag gccttcgacc
    20641 tgatatccct gttgttgcaa cagccaggca gaaacggagg aatcgacacc gccggacatg
    20701 ccgacgatta ctttttttgc ggtttcagac attggatcac tcacgacatt gaacttcaag
    20761 gcggcgtatt ctatcacgca gccttttaga tgacaccctt tgtaaaaggc cagttaaaat
    20821 cgccaatcat ctccagcgga tagcgttgcc cgctttgata acaacgaata ctttccgcca
    20881 ccagcggcga acgaagattt gacgcctgta aaatttcttc ggcgctgacc caacggcagc
    20941 agtcgatatc gctgtcatga ggctgcgtcg ggcatatttg ctcaagctca atggcaaaga
    21001 ggaaacgcaa aaacggcgtt ttatcaggcg caatccactg atgcatacga ataaagtgtt
    21061 gcggctgcgc gctaatgccg gtttcttccc acagctcacg ggcggcggct tccactaagg
    21121 tttcatcggc ttccaggtgc ccggcaggtt ggttccataa cgctttacca ttaatcgtct
    21181 cttcaacgac taaaaatttg ccttctgcgt gcaccacgca agcaacggta acgtgcggtt
    21241 taaacatttt gcatccttaa tctgtcgcgt ctcgccattc gccattggca agattatcca
    21301 aagagtaatt ccccatcgca tagcgaatca gtcgcagcgt ggggaaacca acatgggcgg
    21361 tcatgcggcg cacctggcga ttacgtcctt catataaggt gatcttcagc cagctggtgg
    21421 gaatactttt gcgctcacga attggcggat tacggggcca taaccattcc ggctcctcaa
    21481 ccatctctgc acctgcgggc agggtagggc catcatttaa ggttacgcca ttgcgcaacg
    21541 cttcaagggc gtcttgtgtg ggaatacctt ctacctgtac ataatagatt ttcccggtac
    21601 gtttacccgg ctgggttaaa cgcgcctgca aagcgccgtt attggtcagc accagtaacc
    21661 cttcgctgtc gcggtcgaga cgacctgccg catacacccc ctgaaccggg atgaattctt
    21721 ttaatgtttt gcgtccggct tcatcggtga actgcggaag aacatcgtag ggtttattga
    21781 acaggatcac acgcgtgggc tggttttcag gttttcgtct ggtagaacgt tgcgagctga
    21841 atcgcttaac cttgtgattt ctaaaagaag ttttttgcat ggtattttca gagattatga
    21901 attgccgcat tatagcctaa taacgcgcat ctttcatgac ggcaaacaat agggtagtat
    21961 tgacaagcca attacaaatc attaacaaaa aattgctcta aagcatccgt atcgcaggac
    22021 gcaaacgcat atgcaacgtg gtggcagacg agcaaaccag tagcgctcga aggagaggtg
    22081 aatggaaagt aaagtagttg ttccggcaca aggcaagaag atcaccctgc aaaacggcaa
    22141 actcaacgtt cctgaaaatc cgattatccc ttacattgaa ggtgatggaa tcggtgtaga
    22201 tgtaacccca gccatgctga aagtggtcga cgctgcagtc gagaaagcct ataaaggcga
    22261 gcgtaaaatc tcctggatgg aaatttacac cggtgaaaaa tccacacagg tttatggtca
    22321 ggacgtctgg ttgcctgctg aaacccttga tctgattcgt gaatatcgcg ttgccattaa
    22381 aggcccgctg accactccgg ttggtggcgg tattcgctct ctgaacgttg ccctgcgcca
    22441 ggaactggat ctctacatct gcctgcgtcc ggtacgttac tatcagggca ctccaagccc
    22501 ggttaaacac cctgaactga ccgatatggt tatcttccgt gaaaactcgg aagacattta
    22561 tgcgggtatc gaatggaaag cagactctgc cgacgctgag aaagtgatta aattcctgcg
    22621 tgaagagatg ggcgtgaaga aaattcgctt cccggaacat tgcggtatcg gtattaagcc
    22681 gtgttcggaa gaaggcacca aacgtctggt tcgtgcagcg atcgaatacg caattgctaa
    22741 cgatcgtgac tctgtgaccc tggtgcacaa aggcaacatc atgaagttca ccgaaggcgc
    22801 gtttaaagac tggggctacc agctggcgcg tgaagaattt ggcggtgaac tgatcgacgg
    22861 cggcccgtgg ctgaaagtta aaaacccgaa taccggcaaa gagatcgtca ttaaagacgt
    22921 gattgctgat gcattcctgc aacagatcct gctgcgtccg gctgaatatg atgttatcgc
    22981 ctgtatgaac ctgaacggtg actacatttc tgacgccctg gcagcgcagg ttggtggtat
    23041 cggtatcgcc cctggcgcaa acatcggtga cgaatgcgcc ctgtttgaag ccacccacgg
    23101 tactgcgccg aaatatgccg gacaggacaa agtaaaccca ggctctatta ttctctccgc
    23161 tgagatgatg ctgcgccata tgggttggac tgaagcggct gacctgattg ttaaaggtat
    23221 ggaaggcgca atcaatgcca agaccgtaac ctatgacttc gaacgtctga tggaaggcgc
    23281 taaactgctg aaatgttcag agtttggtga cgcgatcatc aagaatatgt aatcactaca
    23341 tgtgttaaat attgaaacgg gcgtataaca cgcccgttgt tttatgatga tgtaaaatct
    23401 tccccaaaac tttccccaaa acccttcccc aaaactggct attttctatg ctgttttgat
    23461 atctacgata atccagtctt taccacgatc atcattgtat cggtcggtca tttttctgga
    23521 tttatggcct aacaactttt gcgtatccag accctgttcc cgatataacc gttctgacag
    23581 agatcgctgc tcatgaaatg tgggcgcagt tccttgctcc cattttatgc cacatttttc
    23641 ccgggccttt ttaaaagccg ttgtcagagt atttgcagaa acctggtctc ctctgtttgc
    23701 ttgagaggta gtgtgacggt aatggaccag atatttacta acaacagcat ccctgcactg
    23761 agatataact tcacgaaggg taatattcag agcatcgcat ttcaggttaa gcggaatagc
    23821 aagttttgaa ccggtttttt cctgagtaat gtgcaacatg tcgtcccata tatcagagaa
    23881 tttcaaattg cagatatcgc ctaaacgttg tccagtaaca agagcaagta gcatgccgca
    23941 ttttaaatag ggctgccgtc tgcttacgct gtcaaatatt gcctgccatt cgggcagtga
    24001 caatctttgg cggtttactc gatttcgcgg ttgttttgtt gcctgcgctg ggttaaatcc
    24061 tggcggaaca tgtcctgcgt gttgtgcttc tttgaagacg tcgatcaaca ccattctcac
    24121 gacttgcgcc atcctgttat gaccttcagc ctttacagca tcaattattt cggcaatatc
    24181 aagtgcggta atatccttg
//
LOCUS       DADTFG010000069        24179 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.069, whole genome shotgun sequence.
ACCESSION   DADTFG010000069 DADTFG010000000
VERSION     DADTFG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.069"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            201..2444
                     /gene="pdeF"
                     /locus_tag="J1138_002544"
     CDS             201..2444
                     /gene="pdeF"
                     /locus_tag="J1138_002544"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBA8454322.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2483..4024)
                     /gene="ppx"
                     /locus_tag="J1138_002545"
     CDS             complement(2483..4024)
                     /gene="ppx"
                     /locus_tag="J1138_002545"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBA8454323.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4029..6095)
                     /gene="ppk1"
                     /locus_tag="J1138_002546"
     CDS             complement(4029..6095)
                     /gene="ppk1"
                     /locus_tag="J1138_002546"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBA8454324.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6267..6905)
                     /gene="purN"
                     /locus_tag="J1138_002547"
     CDS             complement(6267..6905)
                     /gene="purN"
                     /locus_tag="J1138_002547"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBA8454325.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6905..7942)
                     /gene="purM"
                     /locus_tag="J1138_002548"
     CDS             complement(6905..7942)
                     /gene="purM"
                     /locus_tag="J1138_002548"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBA8454326.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8267..8893
                     /gene="upp"
                     /locus_tag="J1138_002549"
     CDS             8267..8893
                     /gene="upp"
                     /locus_tag="J1138_002549"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBA8454327.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8979..10268
                     /gene="uraA"
                     /locus_tag="J1138_002550"
     CDS             8979..10268
                     /gene="uraA"
                     /locus_tag="J1138_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBA8454328.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10318..11064
                     /gene="hda"
                     /locus_tag="J1138_002551"
     CDS             10318..11064
                     /gene="hda"
                     /locus_tag="J1138_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBA8454329.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11170..11529)
                     /gene="arsC"
                     /locus_tag="J1138_002552"
     CDS             complement(11170..11529)
                     /gene="arsC"
                     /locus_tag="J1138_002552"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBA8454330.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEGALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPELVLEIVG"
     gene            complement(11550..13013)
                     /gene="bepA"
                     /locus_tag="J1138_002553"
     CDS             complement(11550..13013)
                     /gene="bepA"
                     /locus_tag="J1138_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBA8454331.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13226..14287
                     /locus_tag="J1138_002554"
     CDS             13226..14287
                     /locus_tag="J1138_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA8454332.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14386..14856)
                     /gene="bcp"
                     /locus_tag="J1138_002555"
     CDS             complement(14386..14856)
                     /gene="bcp"
                     /locus_tag="J1138_002555"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBA8454333.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14856..15428)
                     /locus_tag="J1138_002556"
     CDS             complement(14856..15428)
                     /locus_tag="J1138_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBA8454334.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15574..16452
                     /gene="dapA"
                     /locus_tag="J1138_002557"
     CDS             15574..16452
                     /gene="dapA"
                     /locus_tag="J1138_002557"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBA8454335.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARIINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16469..17503
                     /gene="bamC"
                     /locus_tag="J1138_002558"
     CDS             16469..17503
                     /gene="bamC"
                     /locus_tag="J1138_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBA8454336.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17716..18429
                     /gene="purC"
                     /locus_tag="J1138_002559"
     CDS             17716..18429
                     /gene="purC"
                     /locus_tag="J1138_002559"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBA8454337.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18597..19460
                     /locus_tag="J1138_002560"
     CDS             18597..19460
                     /locus_tag="J1138_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBA8454338.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMDGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19475..21490
                     /locus_tag="J1138_002561"
     CDS             19475..21490
                     /locus_tag="J1138_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HBA8454339.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARQGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQRPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVCTERLCDRITQWQFFH"
     gene            21564..22262
                     /gene="ypfH"
                     /locus_tag="J1138_002562"
     CDS             21564..22262
                     /gene="ypfH"
                     /locus_tag="J1138_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA8454340.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPSGRQWFSVQGITEDNRKARVNAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQKALISAGGDVTLDIVEDLGHAIDNRSMQLALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22343..22543)
                     /locus_tag="J1138_002563"
     CDS             complement(22343..22543)
                     /locus_tag="J1138_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBA8454341.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22571..23698)
                     /gene="dapE"
                     /locus_tag="J1138_002564"
     CDS             complement(22571..23698)
                     /gene="dapE"
                     /locus_tag="J1138_002564"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBA8454342.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNDFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23702..24058)
                     /locus_tag="J1138_002565"
     CDS             complement(23702..24058)
                     /locus_tag="J1138_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBA8454343.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5678 a         6004 c         6327 g         6170 t
ORIGIN      
        1 tgtaataatg atgagttaac atgataaacg taataattca ttaatcaggg ttattttgtt
       61 tgatatatat caattggaac tttcatatga cacttagaat cattgctctc tatagtgatg
      121 aataatcatc attcgaagtc aggtgggatg cctgtctgaa tacacctcct tcaggatgtg
      181 ggggattctg ctgagcatct atgaaactga atgcaactta tataaaaata cgtgataaat
      241 ggtgggggct tccgctgttc ctgccttctt taatcttgcc cattttcgcc cacattaata
      301 ctttcgcgca tatttcttcc ggtgaagttt ttctctttta tctgccactg gcactgatga
      361 tcagcatgat gatgtttttc agctgggcgg cattgccagg gatcgcctta gggatttttg
      421 tccgcaaata tgcagagctg ggtttttacg aaacgctctc attaacggct aattttatta
      481 tcattatcat tctctgttgg ggcggttaca gggtttttac tccccggcgt aacaacgttt
      541 cacatggtga tagccgttta atttcccagc gactattctg gcagattgtg tttcctgcaa
      601 cgctgtttct gatacttttc cagtttgctg cgtttgttgg attactggcg agcagagaaa
      661 atctggtcgg cgtcatgccc tttaacctcg ggaccttaat caattatcag gccttgctgg
      721 tgggtaatct gattggtgtc ccgctgtgct acttcatcat tcgggtggtg cgaaatccgt
      781 tttatttacg tagctattat tcgcaattaa aacagcaggt tgatgccaaa gttaccaaaa
      841 aagagttcgc aatctggcta ctggcattag gtgccttatt gttgctgtta tgcatgccgt
      901 taaatgaaaa aagcacgatt tttagcacca attacacctt gtcattattg ctgcccctga
      961 tgatgtgggg agcgatgcgt tatggttata agctgatttc attgctctgg gcggttgtgt
     1021 tgatgatcag catccacagc tatcaaaatt acattcccat ttatcctggc tataccacgc
     1081 agctgaccat aacctcctcc agttatctgg tattctcttt tattgtcaat tatatggctg
     1141 tactggcaac ccgtcagcga gcggtagtca gacgcattca gcggcttgcg tatgtggacc
     1201 cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc gttacgtgat gccccctggt
     1261 ctgcgctttg ttatttacgc atccctggca tggaaatgct ggttaagaat tatggcatca
     1321 tgctgcggat tcaatacaag caaaaacttt ctcactggct gtcaccattg ctggaaccgg
     1381 gtgaagatgt ttatcagctt tcgggtaacg atctcgcgct gcggctgaat acagaatcgc
     1441 accaggagcg cattaccgca ctggatagcc atctcaagca atttcgtttc ttttgggatg
     1501 gaatgccgat gcaaccgcag attggcgtca gttactgcta tgtgcgctcg ccagtgaatc
     1561 atatctacct gctgctggga gagctaaata cagtggccga actttccatc gtgaccaacg
     1621 ccccggaaaa tatgcagcgt cgcggagcaa tgtatttgca acgcgaattg aaagataaag
     1681 tcgcgatgat gaatcggcta cagcgggcgc tggaacacaa ccattttttc ctgatggccc
     1741 agccgattac cggtatgcgt ggtgatgtct accatgaaat tcttctgcgc atgaaaggtg
     1801 agaatgatga actgatcagc cccgatagct tcttaccggt cgcgcacgaa tttggtttat
     1861 cgtcgagtat cgacatgtgg gtcattgagc atacgctgca atttatggct gaaaacagag
     1921 cgaagatgcc cgctcaccgt tttgctatta atctgtctcc aacctcggta tgtcaggctc
     1981 gttttcctgt tgaagtcagt cagctgctgg ctaaatatca gattgaagcg tggcaactta
     2041 tttttgaagt caccgaaagt aatgctctga ccaatgttaa gcaggcgcaa atcaccttgc
     2101 agcatcttca ggaattaggc tgccagattg cgattgatga tttcggcact ggctacgcca
     2161 gctatgcgcg gcttaaaaat gtgaatgccg atctgcttaa aattgacggc agttttatcc
     2221 gcaatattgt gtcaaatagt ctggattatc agatagtggc gtcgatttgc cacctggcgc
     2281 gaatgaagaa aatgcgggta gtggcagagt acgttgaaaa cgaagagatc cgcgaggcgg
     2341 tgctctcttt ggggatcgat tatatgcagg gttatcttat tggtaagccg caaccgttaa
     2401 ttgatacgct gaatgaaatc gaacccattc gcgaaagtgc ctgaataatg cgggccgaca
     2461 tttctcgtcg gcccgcaaag cattaagcgg cgatttctgg tgtactttct tcttcaattt
     2521 tcaaccgcca gccagccacg ccttcccagt attcttgctc cttttccaga tcaagcagta
     2581 ccagcgcatt ctgactaaac cagtcatgcg ggaaacgcag tgtccagtga ctgtcatcag
     2641 taatcagcgt caatgttggc ggtgtggtgg ttgcctgacg ttgattattg agtaatacgc
     2701 caaggcgcaa tagctgaatc agtggcagga actgtttctt cttaaacaag gtaaagcgcg
     2761 gcagatcgtc gagtttaatc gctttacggt gatagcgcac cagtgtcgcc atcatcagct
     2821 gctgttcctg attaaaaccc ggcaagtcgc tgttttgcag aatataagcg gagtggcgat
     2881 gcaaaccgct gtggttgata ttcaacccga cctcatgcag catggcggcc catcgcagta
     2941 gcgcctccag ttgtggatgc gccagcttcg gttgctgttc ccgccactgt tcgtacattt
     3001 gcatagtggt atccagcacc cgtcgggcct gttcgctgtc gatgtgatac tggttggcga
     3061 ggctgctggc agtgcgacta cgcacatcct gatgacggaa acgtccttcc atttcataca
     3121 gtacgccttc gcgtaacgcc ccgtcagaaa ggcgcagttc acggatggct aaagcatcaa
     3181 acacaccgca taaaatcgcc agtccaggaa cgaagactgt tttccgctct tcggaaagac
     3241 ctggtaaact cagcgatgcg aaattacggt gccgtaaaac ttcttttacc agtttttcca
     3301 gacgttccgg ggtaattatc ccgtctttct cgcccatttc cattaacact tcatgggcgg
     3361 cttttatggt accggaagcg cccattgcca cgttccagcc ctgaattcga aattgccagg
     3421 ttaacgtttc cagtttttgt gctgccgcca tgcgagcgcg ctgaaaattc tctttattga
     3481 tgaccccgcc agggaaataa agctgggcaa agctgacaca acccatccgg cggctttcaa
     3541 cgagaatcgg ttcgaaattt tcaccaatca ccagttctgt agaaccgccg ccaatatcaa
     3601 taaccagttt gcgacctttt tccggttggg tatgttccac gcccataaaa atcagacggg
     3661 cttcttcatt accggaaata atttcgatcg ggtagggaat gactttttcc gcgcgtttca
     3721 gaaagtcagt ggcgttcagc gcctgacgca gcgtatgggt accaactata cagacgctgg
     3781 caggagaaaa cccttgtagc ctttcggcaa atagcgacag acagttcaaa ccgcgcgtca
     3841 ttgcctcttc actcaacata ttatctggcc ccaggccgtc cgccagatgc acccgctgtt
     3901 tcaggcggcc aataatctgc atggcaccat ctaccacacg ggctatgacc atgtgaaaac
     3961 tgtttgaacc aagatcgacc gcagcaaact cctgcggacg aggggattta tcgtgtattg
     4021 gcatagggtt attcagattg ttcgagtgat ttgatgtagt cgtaaatcgc caactgcgcc
     4081 cgtactttgc ggcgattgcc gcggggaacg tagcgattac tgagttcttt atcgatataa
     4141 cgtgctttga ccgtatcgct gaacaatatg tcgatgatgt ccagcacccg ctgcttcagg
     4201 cgcggatcga gcagcggcgt tgccacttca atacgataat caatattgcg cgtcatccag
     4261 tcggcggaag aaaggtagac ctttttatcg ccgccatttt caaaaatata aacccggtca
     4321 tgttcaaggt aacggtcaac aatactgatg gcacgaatgt tgtcgctaat accttccaga
     4381 ttggggatca gagaacacat tccgcgcacc agcagattaa ccggtacgcc ggagctggag
     4441 gccgcataca gacgatcaac caggccttta tcgacaaggt tatttagctt cagggtgata
     4501 ccactgggca gcccttgctg cgcgttggcg atctcgcggt ccaccatttc atacaacaga
     4561 cggcgagagt tttgcggcga caccattaaa taatcaaatg tcaccggacg gtatgggttt
     4621 tcaataaagt tgaacacccg ccgtacttcg ttagtgatgc gcgcatcggc ggtcagcaac
     4681 gaatagtcag tataaagacg cgcggttttt tcgttaaagt tcccggtccc gatgtgtgcg
     4741 taacgcacca cttcaccgtt ttctttacgt gaaatcagga acagtttggc gtgaattttc
     4801 agccccggcg cagagaagat aacgtgcacg cctgcttcgg tcaggcgctt cgcccagtga
     4861 atgttggctt cttcgtcgaa acgcgcctgt aactcaacca ccacggtgac tttcttaccg
     4921 ttgtgtgcgg cgtggatcat cgagtcgatg atgcgtgaat ctttcgccac gcggtaaatg
     4981 ttaattttaa tcgctagtac gctcgggtcg aacgaagcct gacgcagcag ttccagcaca
     5041 tgctcaaagg tgtgataagg ataatagagc aacacatcgc gttcgcgaat ggcatcaaaa
     5101 ccattgcgga actgggcttt atcaaaccaa atatggcgta aacgcggcag tggtttgttc
     5161 accagattgg ctttgccgac attggggaaa ttaataaagt ctttaaaatt atgataacga
     5221 ccaccgggga cgatggagtc gtagcgggaa atagtcagtt tttcgcgtaa cacttcaacc
     5281 agagcattgg gcatatcgcg ctgataaaca aaacgcaccg gctctgcagt taaacgctgc
     5341 ttgagactgg aagacatcaa ctccatcaga ctggcttcca tctcatgcac taaatcgtat
     5401 tcggcatcgc gggtcatctt cattgaatag gcattcagtg cgtcataatc aaagaagcct
     5461 ttgaaaatat catcaaggca gtaacgcaga atgttatcca gaagaatcat cggcttgcgt
     5521 cgacgcggcg cttctggcgg taaattcaca aagcgcggca ctttatctga tgggatctcc
     5581 agcagcgcgt aacggatggt atcgccacgg ataatttcca ccgccagata ggtgtaatca
     5641 tctttcagga actgcactaa gtcagtgtca ggattgatta aaatcggcgt aatgtgctga
     5701 cgcagatact gcttaaaata atgacgcagc cagttttgtt gattgacgga gagctggcgt
     5761 tcattaatca ggaagatctg gttgcgcgcc atctccagca gtagctcgtt gtagaggccg
     5821 tcgaattcct gatcggcttt cagcacccgg gactgaattt tgcccagtaa atggcgggaa
     5881 tgagagttgg aaccttgttc ttcgctaata atgatgcgtc gcttcagttc agcgaagcgg
     5941 actttataga actcatcaag gttattggaa tagatcccca ggaaacgcat cctttcaatc
     6001 agcgggttag atttgtccgc cgcttcctga agcacgcgtt cattgaacga taaccaactg
     6061 agctcttttt cgatgtatag cttttcctga cccattacca cttttactcc gttttattca
     6121 cgggacactg cctggatatt atggcgagca atattggcag atgtccaact atgccagaaa
     6181 agggggaaaa aaactgctgg agcttgcgat ttacaggtga attgaaaaac gccagcggca
     6241 gagccggcgc tttaactacg ggggcattac tcgtcggcag cgtagccctg cggcggcaga
     6301 cgttgaccat ccagccacgc ggcgttttcg tgcattttca gacgaccatc ggcaaaccag
     6361 ctaatcacca gtggataaat agcatgttcc tgagtttgca cgcgggcggt gatgtcatct
     6421 tccgtatcac cagcaaatac cgggactttc gcctgtaaaa taaccgggcc accgtccagt
     6481 tcatcggtga cgaaatgcac cgatgtaccg tgctcctcat cgccattttc cagcgcctga
     6541 cgatgggtgt gtaatccggg atatttcggc agcagagaag ggtgaatgtt cagcaaacgc
     6601 ccggcatagt gggagacaaa cgccgggctg agaatgcgca taaaaccagc cagcacgacc
     6661 acatcgggtg cgtacatgtc gatttcatga atcaactccc ggtcataggc ttcacgactg
     6721 tcaaacgcgc tggtgatgag cgtatgcgtt gcaatacccg cctggcgggc gcgttcaagg
     6781 ccgaacgcgt cggccttatt gctgaaaact gcccgtacgg tgcctttaat tttgttggtt
     6841 ttgcaggcgt caataattgc ctgcaaatta cttccgttgc cggaaataag caccacaata
     6901 ttcattattc gataaccacg cgttgttcgg aatcagaggc tttgatgata ccgattttcc
     6961 acgcgttttc accgttggca ttgagcaggg cgagggcttt gtccacttcc ggagcaggca
     7021 gggcaataat cataccgacg ccgcagttga aggtgcgata catttcatgg cgctcaacat
     7081 tacctgccgt ttgcagccag ttgaacactt ccggccactg ccaggaagat tcatcaatca
     7141 ctgcctgggt attatctggc aatacgcgcg gaatgttttc ccagaagccg ccgccggtca
     7201 ggtgcgcaat ggcatgcaca tcgacctttt caatcaactc cagcactgac ttcacgtaaa
     7261 tgcgggtcgg tgccagcaga tgatcggcta atggcttacc atcaagttcg gtggtttgcg
     7321 gatcacaacc gctgacttca agaattttgc gcaccagcga atagccattc gagtgcgggc
     7381 cgctagagcc gagtgcaatc agcacatcgc cgtcgctgac tttagatccg tcgatgattt
     7441 ctgatttttc taccacgcca acgcagaaac cagcgacatc gtaatcttca ccgtgataca
     7501 tccccggcat ttctgccgtt tcgccaccca ccagtgaaca gcccgattgc agacaacctt
     7561 ccgcaatgcc gctgatcacc gctgaagcgg tatcaacatc cagttttccg gttgcgtaat
     7621 agtcgaggaa aaacagcggt tccgcacctt gcaccaccag gtcattaacg cacatggcga
     7681 ccagatcaat accaatggtg tcgtgacgtt ttaagtccat cgccagacgc agcttggtac
     7741 ctacgccgtc agtgccggaa accagcacag gttcacgata tttttgcggc aatgcacaca
     7801 gcgcaccgaa gccgcccaga ccgcccatca cttccggacg acgcgttttc ttcactacgc
     7861 ctttgattct tccaaccaga gcattacccg cgtcaatatc aacaccggca tctttgtagc
     7921 taagagaggt tttatcggtc actgcttggg tccccacgcg ttacttgcgg tagaaaaata
     7981 aaattcggcg caattctaac agggaaagca aacgtttgcg agactgcttt acacaacctt
     8041 tttgcacgtc ttttccccag gcgcgcggcg aaagaatatt tgtaccaggg caaaggatcg
     8101 ttttcggatg gaataatctt ctttcataac catttgaata taaaataact ttatctcaaa
     8161 ccgttatcat tttgactaaa gtcaacgaaa ataatattgc cgccttgaag aaaggaggta
     8221 taatccgtcg attttttttg tggctgcccc tcaaaggaga aagagtatga agatcgtgga
     8281 agtcaaacac ccactcgtca aacacaagct gggactgatg cgtgagcaag atatcagcac
     8341 caagcgcttt cgcgaactcg cttccgaagt gggtagcctg ctgacttacg aagcgaccgc
     8401 cgacctcgaa acggaaaaag tcactatcga aggctggaac ggcccggtag aaatcgacca
     8461 gatcaaaggt aagaaaatta ccgttgtgcc aattctgcgt gcgggtcttg ggatgatgga
     8521 cggtgtgctg gaaaacgttc cgagtgcgcg catcagtgtc gtcggtatgt accgtaatga
     8581 agaaacgctg gagccggtac cgtacttcca gaaactggtt tctaacatcg atgagcgtat
     8641 ggcgctgatc gttgacccaa tgctggcaac cggtggttcc gttatcgcga ccatcgacct
     8701 gctgaaaaaa gcgggctgca gcagcatcaa agttctggtg ctggtagctg caccagaagg
     8761 tatcgctgcg ctggaaaaag cgcacccgga cgtcgaactg tatactgcat cgattgacca
     8821 gggactgaac gagcacggat acattattcc gggcctcggc gatgccggtg acaaaatctt
     8881 tggtacgaaa taaagaataa aaataattaa agccgacttt aagagtcggc ttttttttga
     8941 gtaaagcgcc tataacacat aatacagagg ataatactat gacgcgccgt gctatcgggg
     9001 tgagtgaaag accgccactt ttacagacaa tcccgcttag tttgcaacat ttgttcgcca
     9061 tgtttggtgc aaccgtcctg gtgcccgtct tatttcatat taacccggcg actgtactgt
     9121 tatttaacgg tataggaaca ctgctgtatc tcttcatctg taaagggaaa attccggctt
     9181 atcttggttc cagctttgcc tttatttcac cggtattgtt actgttgccg ttagggtatg
     9241 aagtcgcgct gggcggcttt attatgtgcg gcgtgctgtt ctgcctggtt tcttttatcg
     9301 tgaagaaagc ggggaccggc tggctggacg tgctgtttcc acctgcggca atgggcgcaa
     9361 tcgttgccgt catcggtctg gagctggcgg gcgtagctgc cggtatggcg ggtttactcc
     9421 cggctgaagg gcaaacgcca gactccaaaa ccatcatcat ctctattacc accctggcgg
     9481 tcacggtttt aggttccgtg ctgtttcgtg gtttcctggc aattatcccg attttaattg
     9541 gcgtgctggt gggctacgcg ctctctttcg caatgggaat tgtcgatacc acgccgatta
     9601 ttaatgctca ctggtttgcg ctgccaactc tctatacgcc gcgcttcgag tggtttgcca
     9661 ttctgactat tctgccagcg gcgttagtgg ttattgccga acacgtaggg cacctggtcg
     9721 taacggctaa tatcgtcaaa aaagatctgc tgcgcgatcc aggtctgcac cgttcgatgt
     9781 ttgctaacgg cttgtcgacc gtgatctccg gcttctttgg ctccacgcca aacaccactt
     9841 acggagaaaa cattggcgtg atggcgatca cccgtgttta cagtacctgg gttatcggcg
     9901 gtgcggcgat tttcgctatc ctgctttcct gcgtcggtaa actggctgct gctatccaga
     9961 tgattccatt gccggtgatg ggcggcgttt cgctgctgct ttacggtgtc atcggtgctt
    10021 ccggtattcg tgtgttgatc gaatcgaaag tggattacaa caaagcacag aacctgatcc
    10081 tgacttccgt tattttgatc atcggcgtca gtggcgcgaa ggtaaacatt ggcgcggcgg
    10141 agctgaaagg tatggcgctg gcgaccatcg tcggtatcgg cttaagtttg atcttcaaac
    10201 tgattagtat gctgcgccct gaagaagtgg tgctggacgc agaagacgcg gatataacag
    10261 acaaataatg taccgaccgg gcagtgttgc tgcccggttc aaacatcatg ggattctgtg
    10321 gtaaacttct cgcgattttg tgaaatcctg gttgaggtat ctctgaacac accggcacag
    10381 ctctctttgc cactttatct tcctgacgac gaaacctttg caagtttctg gccgggggat
    10441 aactcctctt tactggctgc gctgcaaaac gtgctgcgtc aggaacatag cggttacatc
    10501 tatctctggg cacgcgaagg cgcggggcgc agccatctgc tgcacgcggc ttgcgcggaa
    10561 ttgtcacagc gtggcgatgc ggtgggctat gtcccgctgg ataaacgcac ctggtttgtt
    10621 ccggaagtgc tcgacggtat ggagcatttg tcgctggtct gtatcgacaa cattgagtgt
    10681 attgcaggcg atgagttgtg ggagatggcg attttcgatc tctacaatcg aattctggaa
    10741 tcgggcaaaa cacggttgtt gatcaccggc gatcgtccac cgcggcagtt gaatctggga
    10801 ttaccggatc tcgcgtcgcg tctcgactgg gggcagatct acaaattgca gccactttct
    10861 gatgaagata agttgcaggc gctacagtta cgcgcgcgtt tgcgtggttt tgaactgccg
    10921 gaagatgtgg ggcgtttctt gctgaagcgg ctcgacagag aaatgcgcac gttatttatg
    10981 acgctggatc agctggatcg tgcgtcgatt accgcgcaac gtaagctgac cattccgttt
    11041 gtgaaagaaa ttctgaaact atagacaaga cgtttattgt tggatgcgca cccatagtat
    11101 tcgtcggccg gataagacgc ggcaagcgtc gcatccggca atgtgcttca actagcgatg
    11161 gagcacttct taacccacga tttccagtac cagctccggt ggacgaccaa tccgcgcttt
    11221 gccattcgcg accacaatcg ggcgctccat tagctttgga ttatccacca ttgcctgaat
    11281 cagcgcccct tcgctaagcg aactgtcagc caggttcagt tctttataaa gatcctcttt
    11341 ctggcgcatc aattcacgag cgctattcat tcccagcatt ttcagcaaat cacgcagcgt
    11401 tgccgcatcg gcgggtgtct caaggtagag caccacttct ggctccacgc cgttttcttt
    11461 cagcaggttc agcgtttccc ggctctttga acagcgtggg ttatggtaga tttttacctg
    11521 tttggtcatt gttcttcctt taatgcgaat tacatcttgg tataaggctt aaagcgttcc
    11581 tgcagctggc gcaactggtc gatgcgcgca tcgtaacgcg cttgttgcag gctgcctaat
    11641 ttcacctgag aactggcgct actcaacaag gaaatggcct gatcgagtcg cccggcgagc
    11701 gcataacctt ctgctcgcgc agccagttcc tgatcgcggt tatttagtgc ggcttccgcc
    11761 tgtgccagta aatcccagcc gttgctgtca tctttattgt taaaagtgta gcgattcaga
    11821 atattggccg cttcttgtgg ttggccgcct tgtagataag cgttcgccag gttgagctgc
    11881 aacactggat tggtgcgcaa atcacgggca tttttcaagc gattgatcgc ctcattggct
    11941 ttgttttgcc caagatcgat atcagtagcc agatcgagat accatgcgtt accaggttct
    12001 gccgccagta acggttgcag cgttttacgc gcctcgtcgt atttattggc ttccatcgcc
    12061 tgtaaagcac gaccatattg cgccgctcgt tgctgacgaa cgtttccttt cgcccattca
    12121 tccagcaaat cactggtgag ctgattacgt ccggaattat acatccccag tgtgcgcact
    12181 tttgccagat agaaatcttc tgacgattgc accaccatcg ggcgcatctg attggcacgg
    12241 ttacgggcat cagccagacg gctttccggt agtgggtgag tgagcagaat ttctggtggg
    12301 cgcgaagagt aacgcgcctg atcgagtaat ttttcgagga aggtcggcat cgcctgcgga
    12361 tcgaatcccg agcgttgcag cacctgaata ccaatgcggt ccgcttcctg ttcattttgc
    12421 tgggtgaaac tgatcatccc ctggcgcgtt cccgccagtg taccggtcag cgccgccatc
    12481 cccgcctgcg gactggccat cgccagtaaa atagaaccta acgcgccgac ccaggtcagc
    12541 ggcgcgctgc gctgctgatc ttccatcgct cgcgccaggt gacgttgggt gacgtgggag
    12601 atttcgtgcg ccataactga agccagttga ctttcgttat cggaataacg gaacagggca
    12661 gagtgcagca ccacgttgcc gccaaagaaa gcaaaggcgt taatttcgtc gttgttgatc
    12721 agaaagaaat gaaacggtgt cttaaccgaa ttggcatgcg aaaccagacg cattcctagc
    12781 gaattaatgt attgcgttaa cagcggatca ttaattaacg gcgcgctgcc gcgtaactgg
    12841 cggacataat agtcgcccat ctgcatttcc tgaccaatgg aaagcgtgct tcctgcggag
    12901 gttcccatat ccggcaaggt gtctgcgctg tcggcaaacg ccggggctac ctgaccaatg
    12961 gtcatagcag caatgagggt tgcaaccagg ttttttttca actgcctgaa cataacctct
    13021 atcctgtctt tctggagata gtcatttgac cgatactgtg aaatatcgtt cccaccgcgc
    13081 cgcctttgag tgtaacggcg aagattagcc cactcaaatg acttctcagt ttcctgaatc
    13141 cgtcataaaa agcgaagtct tttttgtgac atttcgatac aattgcggat cgcaatcccg
    13201 ctcttgaggt tcagggaagg tctgtatgct cgaaatgttg atgcaatggt atcgccgccg
    13261 ttttagcgac ccggaagcga ttgccttgct ggttattttg gttgctggtt ttggcattat
    13321 ctttttcttt agcggcctac tcgcgccgtt gctggtggct attgtgctgg cctatttgct
    13381 ggaatggccg actgtccgcc tgcagtctat tggctgttcc cgcccttggg cgacatctat
    13441 cgtgctgatc ctgtttgtgg gcatcctgct actgatggcg ttcgtggttc tgcctattgc
    13501 ctggcagcag ggcatctact taatccgcga tatgccaggg atgcttaata aactttctga
    13561 ttttgctgcc acgttaccgc gccggtatcc ggcattaatg gatgcgggga ttattgatgc
    13621 aatggctgaa aatatgcgta gccggatgct gaccatgggc gattcggtgg tgaaaatttc
    13681 cctcgcctcg ttggtcggtt tgctgaccat tgccgtctat ctggtgctgg tgccattgat
    13741 ggtcttcttc ctgctgaaag acaaagagca gatgctgaac gccgttcgcc gggtgctgcc
    13801 gcgcaaccgt ggactggcag gacaggtgtg gaaggagatg aatcaacaaa tcaccaacta
    13861 tatccgcggc aaagtgctgg agatgatcgt ggtagggatc gccacctggc tggggttctt
    13921 gctctttggg ctgaactatt cgctgctgct ggcggtgctg gtcggcttct cggtacttat
    13981 tccgtacatt ggcgcatttg tggtgaccat tccggtggtt ggcgtggcgc tattccagtt
    14041 tggtgctggc acggaattct ggagctgctt cgcggtgtat ctgattattc aggcgctgga
    14101 cggcaacctg ttagtaccgg tgttgttctc cgaagcggtt aacctgcatc ctctggtgat
    14161 tattttatcg gtggtgatct tcggtggttt gtggggattc tggggcgtat tcttcgctat
    14221 tccattggcg acgctgatca aagccgtgat tcacgcctgg cctgatgggc aaatcgcgca
    14281 agactgacct tcagcgaaaa aattttaaac gagtgttgca gggcagtgag aagatttgag
    14341 atgtgctggc cgcaagcgca gccagcacgg aatggagcaa agtaatcagg cgtgttcttt
    14401 cagccagttc agcacaacgt cgtggtgatt gctggttttg aaatcgtcaa agacatgttc
    14461 gattttgcca tcagcgtcaa tcaggaagct gatgcgatga ataccatcgt aggttttgcc
    14521 catgaaggac ttttcacccc agacgccgaa ttgttcgcac acctggtggt cctcatcaga
    14581 caggagcgta aagttaagca gctctttttc cgcaaaacgg gagagttttt cgggtttatc
    14641 ggtgctgata cccagcacat caacgcccgc ttttttcaac tcatccatgt tatcgcgtaa
    14701 gccgcaggcc tgtacggtac agccgggggt catggctttc gggtagaaat aaaccagaac
    14761 acgctgtccc tggaagtcgg tcaaattaac ttgttctccg tcttgatccg gcaagctaaa
    14821 tttcggtgcg atatcaccgg ctttcagtgg attcattact taactccatc ctgttcatca
    14881 tgttgggaat aattgacgac gttaatactg ccttgtgcat tgagttctgt acatagggct
    14941 ttgaacgctt gctcaatatt tgctgcgtcc gcagatgcgg ggctgtgggc agttatctga
    15001 atatgcaact gcgcagccct ttcattttca gcaggttgcg tgcgcgacac cagctccgca
    15061 atgttcatat gatgcgcgtc gaaaagtgct gtgaagcgtt caattaaatg cggggagtct
    15121 gccacatcga cctgaaccca gacagatgct ggcattggcg gacgcggacg cgccgtcgtg
    15181 cgcttcatca cgattaaaag atccagctcg gcacctttca acggtaacgt tgattcaatc
    15241 agagtaatgg cattccatga accggaaagc agcatgataa acgtgaactc ttctcccagc
    15301 atcgccaggc gactgtcttc aatattacag ccgcaactac tgacatgacg ggtgatggtg
    15361 ttcactattc cagggcgatc ggcacccaac gcagtgatca ccagataatg ttgcgatgac
    15421 agtgtcaaac tggttattcc ttcaaggggt gagttgttct taaggaaagc ataaaaaaaa
    15481 catgcataca acaatcagaa cggttctgtc tgcttgcttt taatgccata ccaaacgtac
    15541 cattgagaca cttgtttgca cagaggatgg cccatgttca cgggaagtat tgtcgcgatt
    15601 gttactccga tggatgaaaa aggtaatgtc tgtcgggcta gcttgaaaaa actgattgat
    15661 tatcatgtcg ccagcggtac ttcggcgatc gtttctgttg gcaccactgg cgagtccgct
    15721 accttaaatc atgacgaaca tgctgatgtg gtgatgatga cgctggagct ggctgacggg
    15781 cgcattccgg tgattgccgg gaccggtgct aacgctactg cggaagccat tagcctgacg
    15841 cagcgcttca atgacagcgg tatcgtcggc tgcctgacgg taacccctta ctacaatcgt
    15901 ccgtcgcaag aaggtttgta tcagcatttc aaagccatcg ctgaacatac tgacctgccg
    15961 caaattctgt ataatgtgcc gtcccgtact ggctgcgatc tgctcccgga aacggtgggc
    16021 cgtctggcga aagtaaaaaa tattatcgga atcaaagagg caacagggaa cttaacgcgt
    16081 gtaaaccaga tcaaagagct ggtttcagat gattttgttc tgctgagcgg cgatgatgcg
    16141 agcgcgctgg acttcatgca attaggcggt catggggtta tttccgttac ggctaacgtc
    16201 gcagcgcgtg atatggccca gatgtgcaaa ctggcagcag aagggcattt tgccgaggca
    16261 cgcattatta atcagcgtct gatgccatta cacaacaaac tatttgtcga acccaatcca
    16321 atcccggtga aatgggcatg taaggaactg ggtcttgtgg cgaccgatac gctgcgcctg
    16381 ccaatgacac caatcaccga cagtggtcgt gagacggtca gagcggcgct taagcatgcc
    16441 ggtttgctgt aaagtttagg gagatttgat ggcttactct gttcaaaagt cgcgcctggc
    16501 aaaggttgcg ggtgtttcgc ttgttttatt actcgctgcc tgtagttctg actcacgcta
    16561 taagcgtcag gtcagtggtg atgaagccta cctggaagcg gcaccgcttg cggagcttca
    16621 tgccccggct ggaatgatct tgccggtgac ctccggcgat tatgcaatcc cggtgaccaa
    16681 cggtagtggt gccgtcggta aggcgctgga cattcgtcca ccagcccagc cgctggcact
    16741 ggtttctggc gcgcgtaccc agttcacggg cgataccgct tcattgctgg tggaaaatgg
    16801 tcgtggcaat actctgtggc cgcaggtggt tagcgtgctg caggcgaaaa actacaccat
    16861 cacccaacgt gatgatgctg gtcagacact gaccaccgat tgggtacaat ggaaccgtct
    16921 ggacgaagac gagcagtatc gtggtcgtta tcaaatctct gttaagccgc agggttatca
    16981 gcaggcggtt acggttaaac tgctgaacct ggaacaggcg ggcaaaccgg ttgcagacgc
    17041 ggcgtccatg cagcgttaca gcacggagat gatgaacgtt atttccgccg gtctggataa
    17101 atctgccact gacgccgcga acgctgcgca aaatcgtgcc tccaccacta tggacgtaca
    17161 aagtgcagct gatgacaccg gtttaccaat gctggtcgta cgcgggccgt tcaatgtggt
    17221 ttggcaacgt ctgccagctg cgctggaaaa agtgggcatg aaagtgactg acagcacccg
    17281 ttcgcagggc aacatggccg taacttataa gccgctgtct gacagcgaat ggcacgaact
    17341 gggcgcgagc gatccaggtc tggcatccgg tgactataaa ctgcaggttg gcgatttaga
    17401 taaccgcagc agcctacagt tcatcgatcc gaaaggtcat actctgactc agagtcagaa
    17461 cgacgcgctg gtagctgtct tccaggctgc gtttagcaag taaaaataca gggctggagt
    17521 catccggccc ttttttctga tatgatacgc aaacgtgtgc gtcggcagaa aaacgcgatt
    17581 ttagcggtaa ttcgcacgaa atttgtttgt cggacgtagg tcggataagg cgttcacgcc
    17641 gcatccgaca aaacatccgg cacaccagac agcaaaagat tttaaaacgt taattcacac
    17701 ccaggagtga taaagatgca aaagcaagct gagttgtatc gtggtaaagc gaaaaccgta
    17761 tacagcacgg aaaacccgga cctgttggtg ctcgaattcc gcaatgatac gtcagcaggg
    17821 gatggcgcgc gcattgagca gtttgatcgc aaaggtatgg tgaacaacaa gttcaactac
    17881 ttcattatga gcaaactggc tgaagcgggt atcccgactc aaatggagcg tctgctctcc
    17941 gataccgaat gtctggtgaa aaagctggat atggtgccgg ttgagtgtgt cgtgcgtaac
    18001 cgtgctgctg gctctctggt gaaacgtctt ggaatcgaag aaggtattga gctgaacccg
    18061 ccactgttcg atctgttcct gaaaaacgac gccatgcacg atccgatggt caacgaatct
    18121 tactgcgaaa cctttggctg ggtgagcaaa gagaacctgg cgcgtatgaa agagctgacc
    18181 tacaaagcga acgacgtgct gaaaaaactg ttcgatgatg ctggtctcat tctggtcgac
    18241 ttcaagctgg agtttggcct gtacaaaggt gaagtggtac tgggcgatga gttctccccg
    18301 gacggcagcc gcctgtggga caaagaaacg ctggagaaaa tggacaaaga ccgtttccga
    18361 cagagcctcg gtggcctgat cgaagcctat gaagccgttg cccgccgcct gggtgtacag
    18421 ctggactgat ttttctgttc atcatcttgc cgtgctgctg gcacggcaag acaaccgctc
    18481 tcataagatg tgcattgaac gctcattcct ccgccatttc atcccgcttt cctgtggtaa
    18541 tcttcctctg aaccacctac gatcatttct ataatgaata taatgttgag gtatctatgc
    18601 gttggcaagg gcgacgtgaa agtgacaatg ttgaagacag gcgcaacagc tctggtggtc
    18661 cgtctatgga cggtcccggt tttcgcctgc caagcggtaa aggcgggctg attttactga
    18721 tagtcgtgct ggttgcaggc tactatggcg ttgatttaac cgggttgatg accggacagc
    18781 cggtttccca acagcaatca acgcggtcaa ttagccctaa tgaagacgaa gccgcaaaat
    18841 tcacctcggt gattctggca accacggaag acacctgggg acagcaattc gagaagatgg
    18901 gtaaaaccta tcagcaaccg aagctggtca tgtaccgtgg aatgacgcgt accggctgcg
    18961 gggcgggaca gtccattatg gggccattct attgcccggc ggatggcaca gtttatatcg
    19021 atctctcctt ctatgatgac atgaaagaca aacttggcgc ggatggcgat tttgcccagg
    19081 ggtacgttat cgctcatgaa gtcggtcatc atgtgcagaa actgctaggc atcgagccga
    19141 aagttcgtca actgcaacaa aacgcgacgc aggcggaagt aaaccgctta tctgtgcgta
    19201 tggaactcca ggccgactgt tttgccggtg tatgggggca tagtatgcag cagcaaggtg
    19261 ttctggaaac tggcgatctg gaagaggcgc tgaacgcggc gcaggccatc ggcgatgacc
    19321 gtttacaaca gcaaagtcag gggcgagtag taccagacag tttcactcat ggcacttctc
    19381 agcaacgcta tagctggttt aaacgtggtt tcgacagcgg cgatccggca caatgcaata
    19441 cttttggtaa aagcatttaa cttccggggc agggatggct gaactgactg cgcttcacac
    19501 attaacagcg caaatgaaac gtgaagggat ccgccgcttg ctggtgttga gcggggaaga
    19561 gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc ttacctggcg actggctgtg
    19621 gatttcgccg cagccagatg ctgaaaacca ctgttcaccc tcggcgctac aaactttact
    19681 tgggcgcgag ttccggcatg cggtattcga cgcccgccaa ggctttgatg ccgctgcctt
    19741 tgcggcactt agcggaacgt tgaaagcggg aagctggctg gtgttgttac tccctgtatg
    19801 ggatgagtgg gaaaatcaac ctgatgccga ctcgctgcgc tggagtgatt gccctgaccc
    19861 tattgcgacg ccgcattttg tccagcattt caaacgcgta cttacggcga ataacgacgc
    19921 tatcctctgg cggcaaaacc agccgttctc gttggcgcat tttactcccc gtactgactg
    19981 gcaccccgct accggcgcac cacaaccaga acaacagcaa ctcttacagc agctactgac
    20041 catgccgccg ggcgtggcag cggtaactgc tgcgcgtggg cgcggtaagt cggcgttggc
    20101 agggcaactc atttctcgta ttgcgggcag tgcgattgtc accgcgcccg caaaagcggc
    20161 aacggatgta ctggcacaat ttgcgggtga gaagtttcgc tttattgcac cggatgcctt
    20221 gttagccagc gatgagcaag ccgactggct ggtggtcgat gaagccgcag ctatacctgc
    20281 gccgttgttg tatcaactgg tatcgcgttt tcctcgaacg ttgttaacca ctacggtgca
    20341 gggctacgaa ggcaccggac gtggtttttt gctgaaattt tgcgctcgct ttccgcattt
    20401 acaccgtttt gaactgcaac ggccgatccg ctgggcgcag ggatgcccgc tggaaaaaat
    20461 ggttagtgag gcactggtgt ttgacgatga aaacttcacc catacaccac aaggcaatat
    20521 cgttatttcc gcatttgaac agacgttatg gcgaagcgag ccagaaacgc cgttaaaggt
    20581 ttatcagtta ttgtctggtg cgcactaccg gacttcgccg ctggatttac gccggatgat
    20641 ggatgcacca gggcaacatt ttttacaggc ggctggcgaa aacgagattg ccggggcgct
    20701 gtggctggtg gatgagggcg gattatctca acaactcagt caggcggtat gggcaggttt
    20761 tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg gcgcacggca gcaatccact
    20821 ggcggcgaca ttgcgcggaa ggcgggtcag ccgaatagca gttcatccgg ctcgtcagcg
    20881 ggaaggcaca ggtcagcaac tgattgctgg tgctttgcaa tatacgcagg acctcgacta
    20941 tctttcggtg agttttggtt acaccgggga attatggcgt ttctggcaac gctgcggttt
    21001 tgtgctggtg cgaatgggta atcatcgtga agccagcagc ggttgctata cggcgatggc
    21061 actgttaccg atgagtgatg cgggtaaaca gctggctgaa cgtgagcatt accgtttacg
    21121 tcgcgatgcg caagctctcg cgcagtggaa tggcgaaatg cttcctgttg atccactaaa
    21181 cgatgccgtc ctttctgacg acgactggct tgaactggcc ggttttgctt tcgctcatcg
    21241 tccgctatta acgtcgttag gttgcttaat gcgtctgtta caaaccagcg aactggcatt
    21301 accggcgctg cgtgggcgtt tacagaaaaa cgccagtgac gcgcagttat gtaccacact
    21361 taaactttca ggccgtaagc tgttactggt ccgtcaacgc gaagaagccg cgcaggcgct
    21421 attcgcactt gatgatgttt gcactgagcg tctgtgcgat cgcataacgc aatggcaatt
    21481 ttttcactga ctccttcaat tttctcccat ggtcattgtg agtcagcggc gtaaaattaa
    21541 tgacatcagt taaggagttc gccatgaaac atgaccattt tgttgttcaa agcccggata
    21601 aaccagcaca acagttgtta ctgcttttcc acggtgtcgg ggataacccg gtggcaatgg
    21661 gggaaatagg tagctggttt gcgccgctgt ttcctgatgc gctggtggtg agtgtcggtg
    21721 gcgcggagcc gagcggtaat ccgtcggggc gtcagtggtt ttcggtgcag ggtattacgg
    21781 aagataatcg caaggcgcgt gtgaatgcca tcatgccgac gtttattgag acggtgcgct
    21841 actggcagaa acaaagcgga gtaggggcta atgccacggc gctaatcggt ttctcacaag
    21901 gcgcgattat ggcgctggag agcattaaag ccgaaccggg ccttgcttcg cgtgtcattg
    21961 ccttcaacgg acgctacgcg agcctgccgg aaaccgcgtc tacagccaca acaattcact
    22021 tgattcatgg cggtgaagat ccggttatcg atctggcgca tgcggttgcg gcgcagaaag
    22081 cgttaatcag tgccggtggc gatgtgacgc tggatatcgt ggaggattta ggccatgcga
    22141 tcgacaatcg cagtatgcaa ttagccctgg atcacttgcg ttataccatt ccgaaacatt
    22201 atttcgacga agcgctaagc ggcggtaagc ctggcgatga tgatgtgatt gaaatgatgt
    22261 aataatagcc gggtaggtct gataagactg tatcaggcat catggccgga tgcggcgtta
    22321 acgccttatc cggccactta gcttacttct tcggttgatc ctttttcggc cagtcgtcgt
    22381 catcgtccca cttatcgttg aaatcacgat gcgggggaag ctccggcttg ttggcgagaa
    22441 atttcttatg gtcgacgcgc ttgaggtctt taatcacgtt cagcaggacg cctaccaaaa
    22501 agacaatcac cagaatccac caatatttag ccagccagtc catgcttatt tcctcttgca
    22561 gaaccactca tcaggcgacg agctgttcca tgatacgttg atacatacgg gcaagtagct
    22621 gcaggtcggc agcgttcaca cattcattaa ttttatgaat cgtggcattg accggcccga
    22681 gttccaccac ctgcgccccc atgcgggcaa taaagcgccc gtcggacgtt ccgcctgtgg
    22741 tcagtagctg cggtttaatt tcattatagt gctcaaccgc gttaacgacc gcatccacca
    22801 gtttaccgcg cgcggtcaaa aatggctgcc cggaaagcca ccaatccacc gtatagcgca
    22861 gttgatgttt ttcaagcagg gcaagcacct gcgctttgat catctcatcc gtcagttcgg
    22921 tgctaaagcg gaagttaaac tgcacaaaca gttcacctgg aataacgttg ttactgcccg
    22981 ttcccgcctg aatattggca atctgcatac tggtcgccgg gaagaagtca ttaccctgat
    23041 cccactcaat agccactaat tcattaatga aaggtgctgc gcgatgtacc ggattgtcag
    23101 ccagatgtgg gtaggcaaca tgcccctgaa cgccatgaat ggtaaggttg caggttaacg
    23161 atccgcgacg accatttttc accacatcac ctaccacttc gatactcgac ggttcgccaa
    23221 ccagacagta atcgagacgc tcattacgtg ccattaacgc ttcgacgact tttaccgtac
    23281 cgttgtgggc actagcttct tcatcagagg tgatcagaaa tgccagtcgc cctgtatggt
    23341 tgggatgttg tgcgacaaag cgttctgccg ctaccaccat cgccgccagc gagcctttca
    23401 tatctgccgc accgcgcccg aataacatgc catcacgaat ggtgggttca aacggcggat
    23461 tgatccaacg gtcggcgtcg ccaggcggca ccacgtcggt atgcccggca aaggctaacg
    23521 tttcaccctg cccacgccat gcccaaaaat tctgcgtgtc ggcaaagtcc atgcgttcaa
    23581 cggtaaaacc gatcgcctgc aaacgttcaa tcaacaaagc ctggcatcct gcgtcatcag
    23641 gactcaggga agggcggcga ataagctgtt gtgtcagctc aataaccggg cacgacatag
    23701 actacacctc atggaaaaat tgctgataac tggattcact gaaacccagc agcataggct
    23761 taccaggcgc gcagagcaat ggacgtttga taattgcagg catttcagtc attaatgtcg
    23821 ccgcagaggc cgcatcggtg attttattgc gggtggtttc gtccagttta cgccaggttg
    23881 taccacgggt gttgagtaac gcttcccagc ctaattcgtt gataaaaccg ttcaataatt
    23941 cgctgtccaa tccatcgacg cggtaatcat gaaaacgata gtcgatgtta ttagcttcca
    24001 gccaacggcg agccttctta atggtgtcac aatttttgat gccgtaaagt gtaaccataa
    24061 tgaatccttt ttcgatttat gagaataaat actcatttaa aggtgaaaac attttgcctg
    24121 caaatgatta tcaggaaata taacattatc gaagtattca aattaacaac cattaaata
//
LOCUS       DADTFG010000070        24100 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.070, whole genome shotgun sequence.
ACCESSION   DADTFG010000070 DADTFG010000000
VERSION     DADTFG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.070"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            44..805
                     /locus_tag="J1138_002566"
     CDS             44..805
                     /locus_tag="J1138_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA8454344.1"
                     /translation="MRIFSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            942..2279
                     /gene="adiC"
                     /locus_tag="J1138_002567"
     CDS             942..2279
                     /gene="adiC"
                     /locus_tag="J1138_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBA8454345.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2383..4026
                     /gene="eptA"
                     /locus_tag="J1138_002568"
     CDS             2383..4026
                     /gene="eptA"
                     /locus_tag="J1138_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBA8454346.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNITALNLPGQCINGECYDEVLFHGLEEYINNLQSDGLIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4023..4691
                     /gene="basR"
                     /locus_tag="J1138_002569"
     CDS             4023..4691
                     /gene="basR"
                     /locus_tag="J1138_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBA8454347.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4692..5792
                     /gene="pmrB"
                     /locus_tag="J1138_002570"
     CDS             4692..5792
                     /gene="pmrB"
                     /locus_tag="J1138_002570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBA8454348.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5768..5857)
                     /gene="pmrR"
                     /locus_tag="J1138_002571"
     CDS             complement(5768..5857)
                     /gene="pmrR"
                     /locus_tag="J1138_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBA8454349.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5969..7471)
                     /gene="proP"
                     /locus_tag="J1138_002572"
     CDS             complement(5969..7471)
                     /gene="proP"
                     /locus_tag="J1138_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBA8454350.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7735..8613)
                     /locus_tag="J1138_002573"
     CDS             complement(7735..8613)
                     /locus_tag="J1138_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454351.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNFV"
     gene            complement(8610..10864)
                     /locus_tag="J1138_002574"
                     /pseudo
     CDS             complement(8610..10864)
                     /locus_tag="J1138_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
     gene            11267..11602
                     /locus_tag="J1138_002575"
     CDS             11267..11602
                     /locus_tag="J1138_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBA8454352.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11762..12205
                     /gene="yjdN"
                     /locus_tag="J1138_002576"
     CDS             11762..12205
                     /gene="yjdN"
                     /locus_tag="J1138_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBA8454353.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            12338..12997
                     /gene="phnC"
                     /locus_tag="J1138_002577"
     CDS             12338..12997
                     /gene="phnC"
                     /locus_tag="J1138_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBA8454354.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKA
                     A"
     gene            13022..14038
                     /gene="phnD"
                     /locus_tag="J1138_002578"
     CDS             13022..14038
                     /gene="phnD"
                     /locus_tag="J1138_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8454355.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14144..14923
                     /gene="phnE"
                     /locus_tag="J1138_002579"
     CDS             14144..14923
                     /gene="phnE"
                     /locus_tag="J1138_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBA8454356.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            14944..15669
                     /gene="phnF"
                     /locus_tag="J1138_002580"
     CDS             14944..15669
                     /gene="phnF"
                     /locus_tag="J1138_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBA8454357.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     CFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15670..16122
                     /gene="phnG"
                     /locus_tag="J1138_002581"
     CDS             15670..16122
                     /gene="phnG"
                     /locus_tag="J1138_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBA8454358.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLITPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16119..16703
                     /gene="phnH"
                     /locus_tag="J1138_002582"
     CDS             16119..16703
                     /gene="phnH"
                     /locus_tag="J1138_002582"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBA8454359.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVNQPKQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            16703..17767
                     /gene="phnI"
                     /locus_tag="J1138_002583"
     CDS             16703..17767
                     /gene="phnI"
                     /locus_tag="J1138_002583"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBA8454360.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEYSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMAMVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            17760..18605
                     /gene="phnJ"
                     /locus_tag="J1138_002584"
     CDS             17760..18605
                     /gene="phnJ"
                     /locus_tag="J1138_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBA8454361.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMSALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQN
                     EAKSQ"
     gene            18602..19360
                     /gene="phnK"
                     /locus_tag="J1138_002585"
     CDS             18602..19360
                     /gene="phnK"
                     /locus_tag="J1138_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBA8454362.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19471..20151
                     /gene="phnL"
                     /locus_tag="J1138_002586"
     CDS             19471..20151
                     /gene="phnL"
                     /locus_tag="J1138_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBA8454363.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTHLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            20148..21284
                     /gene="phnM"
                     /locus_tag="J1138_002587"
     CDS             20148..21284
                     /gene="phnM"
                     /locus_tag="J1138_002587"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBA8454364.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            21284..21841
                     /gene="phnN"
                     /locus_tag="J1138_002588"
     CDS             21284..21841
                     /gene="phnN"
                     /locus_tag="J1138_002588"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBA8454365.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            21828..22262
                     /gene="phnO"
                     /locus_tag="J1138_002589"
     CDS             21828..22262
                     /gene="phnO"
                     /locus_tag="J1138_002589"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBA8454366.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            22264..23022
                     /gene="phnP"
                     /locus_tag="J1138_002590"
     CDS             22264..23022
                     /gene="phnP"
                     /locus_tag="J1138_002590"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBA8454367.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCDCVACARARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(23050..23379)
                     /gene="yjdP"
                     /locus_tag="J1138_002591"
     CDS             complement(23050..23379)
                     /gene="yjdP"
                     /locus_tag="J1138_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBA8454368.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQRQVSDDRRKQYNDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23448..23897)
                     /gene="rpiB"
                     /locus_tag="J1138_002592"
     CDS             complement(23448..23897)
                     /gene="rpiB"
                     /locus_tag="J1138_002592"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HBA8454369.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         5757 a         6736 c         6253 g         5354 t
ORIGIN      
        1 tttagcgaga actggtcttt tattcgcact caggagtaca tgtatgagga ttttcagcga
       61 ccaaccttgt attgttttat tgactgaaaa agatgtctgg ataagggtga atgggaaaga
      121 acctattagc cttaaagcta accacatggc gttattaaat tgtgaaaata atattatcga
      181 cgtctcctct cttaataaca ctttggttgc tcatattagt cacgacatca tcaaagatta
      241 cctccggttt ctgaataaag atctctcgca aataccagta tggcaacgta gcgctacgcc
      301 catcctcacc ctgccatgcc tgacgccaga cgtctttcgc gttgccgcgc agcatagcat
      361 gatgcccgct gaaactgagt cagaaaagga acgaacacgt gcattattat tcactgtgct
      421 atcccgtttt ctcgacagta aaaaattcct ttcactaatg atgtatatgt tacgtaattg
      481 tgtaagtgac agcgtttatc aaattattga aagcgatatc cacaaagact ggaatcttag
      541 tatggtagcc agttgtttat gtcttagccc aagtctgtta aagaaaaagc tgaaaagcga
      601 aaacaccagt tatagccaaa taatcaccac ctgccgcatg cgttatgccg taaatgaatt
      661 aatgatggac ggtaaaaata tctctcaggt atcacagtcc tgcggctaca acagtacgtc
      721 gtactttatt tctgtcttta aagacttcta cggtatgacg ccgctgcatt atgttagtca
      781 gcacagagaa cgcactgtcg cctgattttt aaccttaacg aagagctata ttaataacgg
      841 catcagcgat aacccggccg ataataattc aactatcgaa tgcaggcgta tgatatgacg
      901 taatttattg tcacgaagct cgccttcgca ggagtttaat tatgtcttcg gatgctgatg
      961 ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc ggggaatatt atggggtcag
     1021 gtgtttttct gttacctgca aacctggcct ccactggcgg gattgccatt tatggatggt
     1081 tggtgacgat tatcggtgcg ctggggctct cgatggtata cgccaaaatg tcgttcctcg
     1141 acccaagtcc tggtggttct tacgcttacg cccgccgctg ctttggcccg tttctcggtt
     1201 atcaaaccaa cgtcctctac tggctggcct gctggatcgg caatatcgct atggtggtca
     1261 ttggcgtagg atatttaagt tacttcttcc cgattctgaa agatccattg gtattaacca
     1321 tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa cattgtcggt ccgaaaatga
     1381 tcacccgcgt acaggccgtt gctaccgtgc tggcgctgat ccccatcgtc gggattgccg
     1441 tatttggctg gttctggttc cgtggtgaaa cctatatggc ggcatggaac gtcagcggcc
     1501 tgggcacctt cggtgcgatt caaagtaccc ttaacgttac gctgtggtcg ttcatcggtg
     1561 tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc gaaacgcaac gtccctatcg
     1621 ccaccattgg cggggtattg attgctgccg tttgctatgt actttctacc accgcgatta
     1681 tggggatgat ccctaatgcc gcactgcgcg tttctgcttc gccattcggt gatgccgcac
     1741 ggatggcgtt gggtgacacc gccggggcca ttgtttcctt ctgcgcagct gcgggttgct
     1801 taggttcact gggcggctgg acgttgctgg cgggtcaaac ggcgaaagcc gctgccgatg
     1861 acggactgtt cccaccgatt tttgcccgtg taaataaagc gggcacgcca gtggcggggc
     1921 tgattatcgt cggtattttg atgaccatct tccagctcag tagcatttcg ccaaacgcga
     1981 ccaaagaatt cggtctggtt tcttccgtgt cggtcatctt tacactggtg ccatatcttt
     2041 acacctgtgc ggcgttactg ctgctcggcc acggtcactt tggtaaagcg cgcccggcat
     2101 acctggcagt tactaccatt gccttcctct actgcatctg ggccgtagta gggtccggcg
     2161 cgaaagaggt tatgtggtca tttgtcactc tgatggtaat caccgccatg tatgcgctga
     2221 attacaaccg gctgcataaa aacccgtatc ccttagatgc accaataagc aaagattaat
     2281 tccccttaat caagcaaaca taaaagccaa ccttaagaac ttaaggttgg cttaattttg
     2341 ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg tgatgttgaa gcgcctacta
     2401 aaaagaccct ctttaaattt attctcctgg ttattacttg ctacttttta tatctcagtc
     2461 tgcctgaata tcgccttttt taaacaggtg ctacaggtgt taccgctgga ttcactgcat
     2521 aacgtgctgg ttttcttgtc gatgccggtc gtcgcattca gcgtgattaa tattgtcctg
     2581 acactaggct ctttcttatg gcttaatcgt ccactcgcct gcctgtttat tctggtcggt
     2641 gcggctgcac aatatttcat catgacctat ggcatcgtca tcgaccgttc aatgatcacc
     2701 aatatcattg atactacgcc ggcggaaagt tatgcgttga tgacgccacg aatgttatta
     2761 acgctggggt taagtggcgt gttggccgcg attattgcct gctggatcaa aatcaaaccc
     2821 accacctcac gcctgcgcag cgttcttttc cgcggtgcca acatcctgat ttctgtgctg
     2881 ctgattctgc tggttgccgc actgttctac aaagactatg cctcgctgtt tcgcaacaac
     2941 aaagagctgg tgaaatcctt aagcccgtcc aacagcattg ttgctagctg gtcgtggtac
     3001 tctcaccagc ggtcagccaa cctgccactg atacgcattg gtgaagatgc tcaccgcaac
     3061 ccattaatgc aaaatgggaa acgcaaaaac ctgaccattt tgatcgtcgg agaaacgtcg
     3121 cgcgcagaga atttctcgct gaatggttat ccacgcgaaa ccaatcctcg cctggcaaaa
     3181 gataacgtgg tctatttccc taataccgca tcttgtggca cagcaacagc cgtctcagtg
     3241 ccgtgcatgt tctcggatat gccgcgtgag cactacaaag aagagttggc acagcaccag
     3301 gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg tgctgtggaa tgacaacgat
     3361 ggcggctgta aaggcgtttg cgatcgcgta cctcaccaga acatcaccgc gctgaacctg
     3421 cctggtcagt gcatcaacgg cgaatgctat gacgaagtgc tgttccacgg gctggaagag
     3481 tacatcaata atctgcaaag cgatggcctc attgtattac acactatcgg cagccacggc
     3541 ccgacctatt acaaccgcta tccgccgcag ttcaggaaat ttaccccaac ctgcgacact
     3601 aacgagatcc agacctgtac ccaagagcaa ctggtgaaca cttacgacaa cacgctggtt
     3661 tacgtcgact atattgttga taaagcgatt aatctgctga aagaacatca ggataaattt
     3721 accaccagcc tggtttatct ttctgaccac ggtgaatcgt taggtgaaaa tggcatctat
     3781 ctgcacggtc tgccttatgc catcgccccg gatagccaaa aacaggtgcc gatgctgctg
     3841 tggctatcgg aggattatca aaaacggtat caggttgacc agaactgcct gcaaaaacag
     3901 gcgcaaacgc aacactattc acaagacaat ttattctcaa ccttattggg cctgactggc
     3961 gttgagacga agtattacca ggctgcggat gatattctgc aaacttgcag gagagtgagt
     4021 gaatgaaaat tctgattgtt gaagacgata cgctgttatt gcagggattg attctggcgg
     4081 cgcaaaccga aggctacgcg tgcgatggcg tgagcaccgc gcggatggcg gaacaaagcc
     4141 ttgaggccgg tcattacagc ctggtagtac tggatttagg cttacccgat gaagatgggc
     4201 tgcattttct tgcccgtatc cggcagaaaa aatacactct gccggtgtta attctcaccg
     4261 cccgcgatac ccttaccgac aaaatcgccg ggctggatgt cggggccgac gattatctgg
     4321 tgaagccttt tgcgctggaa gagttgcacg cccgtatccg cgccctgcta cgacgccata
     4381 ataatcaggg cgaaagtgag ctgaacgttg gcaacctgac gctgaatatg ggtcgccgcc
     4441 aggtatggat gagcggagaa gaactgattc tgacgcccaa agaatatgct ctgctgtcgc
     4501 gcctgatgct taaagcaggc agcccggttc atcgggaaat tctctacaac gatatctaca
     4561 actgggacaa cgagccctcg accaacaccc tggaagtgca tatccataac ctgcgcgaca
     4621 aagtgggcaa agcccgtatc cgcaccgtgc gcggatttgg ctatatgctg gtcgcaaatg
     4681 aggaaaacta attgaatctg atgcgttttc tgcgccgacc aatatcgctg cgccaacggc
     4741 tgatattgac catcggggcc attttgttgg tgtttgagct gatcagcgtc ttctggctat
     4801 ggcatgaaag taccgagcag attcagctgt ttgagcaggc gctacgcgac aatcgcaaca
     4861 acgatcgcca catcatgcgt gagatccgcg aggcggtcgc cagcctgatt gtccccggcg
     4921 tctttatggt tagcctgacg ctatttatct gctatcaggc ggtacgccgc atcacccgac
     4981 cgctggcgga gctgcaaaaa gagctggaag cgcgcaccgc cgataactta acgcccattg
     5041 ccattcatag cgccaccctc gaaatcgatg cggtggtttc ggcattaaac gatctggtca
     5101 gtcgcctgac cagcacgctg gataacgaaa ggttgtttac cgctgacgtc gcgcacgaac
     5161 tgcgaacgcc actggcgggg gtgcgtttgc atctggaact gctggcgaaa acgcatcaca
     5221 ttgatgtagc accgttagtg gcacgacttg atcagatgat ggagagcgtc tcccagctgc
     5281 tgcaactggc gcgtgccgga cagtcatttt cttccggtaa ttatcaacat gtaaaactgc
     5341 tggaagatgt cattctcccc tcgtatgacg aactcagtac catgctcgac cagcgccagc
     5401 aaaccctgct attgccagag agcgccgccg acatcaccgt tcagggtgat gcaaccctgc
     5461 tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg ttatagccca caaggcagca
     5521 acattatgat taagctgcaa gaagatggcg gagcggtcat ggccgttgaa gatgaaggac
     5581 cgggtattga tgagagtaaa tgcggggagt tgagtaaagc gtttgtacga atggacagcc
     5641 gttatggcgg gattggtctg gggttaagta ttgtcagccg cattacacag ttgcatcacg
     5701 ggcagttttt cctgcaaaac cggcaagaga cttccggcac gcgggcctgg atacggctga
     5761 aaaaagatca gtacgtggca aaccagatat aaagaaagct gctgaccacc agcacgctga
     5821 acacggtagt taaactttca taaacacggt ttttcattgc actctcctcc gttaacctgg
     5881 aggagagtat gcgcgtcaga gattaagtga accttaagag ttcaattgac ctgataagac
     5941 agcgtcacat caggccatcc gtttcagctt attcatcaat tcgcggatgt tgctgcacca
     6001 ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc aatatcatcg attttctgct
     6061 cgatattatc gtaatgctcg acgagaattt ccttcgcttc ctgtatatct gacgccgctg
     6121 gtgtcgcacc tttcaacggt cgattcgccg tctctttcat ggttacgccg gtgattaaac
     6181 caaccaccgc cactaccatc aggtaatagg caggcatcat cagattctgc gagctttcga
     6241 ccagccaggc cgccagcgtt ggcgtcagac cggcaaccag caccgaaata ttaaatgccg
     6301 ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc aggcaaggta gaagccataa
     6361 cgcccgtaaa gcagttaagg atcaccgcca gcatcaataa gccggcaaaa atcaggccga
     6421 tgacgttact gttaatcaga ataaacgccg ggatcgccaa cacaaacagt gcaacactac
     6481 caagaagcac aaacggacga cggccaaaac ggtcactcag caagcccatc accggttgaa
     6541 caaacagcat accgatcata atggcgataa taatcagcac cccgtggtct tcggagtaat
     6601 gcaggttatg cgacaaataa ctcggcatat aggtcagcaa catgtagtaa gtcacgttgg
     6661 tggcaattac cagaccaata catgtcaaca ggctgcgcca gtgtttggtg gcaatctctt
     6721 taaacgagac tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc agtttatcga
     6781 catgctgctg gaacgccgga gtctcttcca gcgcatggcg caggtaaagc ccgataatcc
     6841 ctaacggcag agcgataaag aacggaatac gccagcccca atcgaggaag ttctcttcgc
     6901 cgacaatggt cgaaattaac accactacgc cagctcccag cacaaacccg gcaatagaac
     6961 caaagtccag ccagctgccc ataaagccac gtttacggtc aggggagtat tccgcaacaa
     7021 atattgacgc cccggtatat tcaccgccga ccgagaaacc ttgtgccatc ttacagatca
     7081 acagcagaat cggtgcccaa ataccaatcg tgtcgtagga cggtattaag ccaatacaga
     7141 acgtactgat cgacataatc acaatagtga tagcgaggat cttctggcga ccatatttat
     7201 cgcccaacat accaaagaag agcccgccaa gcggtcgaat cagaaaggga acggagaaag
     7261 tggcgagtgc agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa actttaccta
     7321 atgcgtaagc aacaaaacca taaacaccaa aatcgaacca ttccattgca ttacccagtg
     7381 atgctgcggt aatggcttta cgcagtttac cgtcatcaat aatggtgaca tcacgaaggg
     7441 taatcggttt tacttttttc cttttcagca tagctttcct cgcagagttg gcccatttcc
     7501 gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt gcaggatgca
     7561 atctctgtaa caagcgtagc gggtttgctt acaccctccg gttaaggaat gtacaatcaa
     7621 accagttgac gcctgaattg ggcagttaat gaccttttta ccctaccagt gtttaaattt
     7681 gtgatcaact tcacacatgt atttcgttta ggactcattg gtgtaactga ttatttacac
     7741 aaaatttaca gagaagattt cctcgaccat tgcccccgcc agacgctgcg ctgaacagag
     7801 tcgtggcatc cccagtgcga cagttcgcca gcgttgggtc actgaccagc aaacgggttg
     7861 tttatctgcc gtagaccact ttcccagcca acttaaaaaa tcagcatgat ccccgcccgt
     7921 cgaaacggtc gaagaggcaa accactgatg gtaatagtga cgcgtggtcg gggccgcatt
     7981 gatactctct gccaaatgtt gtgtcgcttt cacgcgcaaa tcacccagca acaacgccac
     8041 cgcttgcggg cttcccagac ggcaggcatc gccaaatgca ccccagcgca actcctccag
     8101 aacgataaag cagcgcccag gcaacgacca ggcgttatac gccccagcac gccagcgggt
     8161 aaaaatttgc tcgctctgtt cacccgcctg caccaatagg cctcgttgcg ttaacgcctt
     8221 ttccttccag cgacaacggg ctgaaaacca ctcatacaag gcggctattt gctgggggag
     8281 ctcagggaaa gcagagtgct ggcggtgatc cattttttgc agcagcgcca gatgttgcgc
     8341 gatctgcgtc agcgccagat gaccgggatc cagcatcgac gccagttttt ccaccaatgt
     8401 cagacgcagg ctaagcggcg catttaacat cccgccgttc gtccatgtct gaagctgcac
     8461 gcgcgcaaga agttcctgca tcagtcgttc acgaaactgc tgctgatggg cttgtggaag
     8521 gcgggcatca tcgccctgcg ccagatccac taaaaagcgc ggatgaaccg actccagcac
     8581 gcgaccgggg ccgtccagta acgtcttggt caatatcgtt ctgccgtgaa aagtgtttga
     8641 atatcatcgc gtaacagctg agcgtcggtg taaatccagc cgtgagtcat cacagtctgc
     8701 tgcaattgct gttgcatcag cctgaccacg gattcatttt gttgacgcag agccagactt
     8761 tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga actcagcgaa aaaagtattc
     8821 atgcctgccg taacagattc atcgacctgc tctgccataa ctttagccat ttgttggcaa
     8881 taaagatcga cttctgcgct taatgctcgt tgcaacacac tgtaatcaac cgtctctgtc
     8941 gggaatttct catttccccg tccccagtcg ggctgattca accagcgcga aaaaatctca
     9001 cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctg cctggcgcga aatgacttca
     9061 ctgaacagct ggcgggtgtt gaagtgggga actacgccgt gaaaaacagg aaaatgaaac
     9121 ccaggacgaa acccagactc actcaattcc attttgactt gttgctcaat ggggcgaata
     9181 acatcggtta acacccggca aagggtggac tcaagctcgg caaagcgcag cgtaaagtcg
     9241 cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac agcgggtacg catctcactt
     9301 aacgactccg aatcatcctg aaacaacgcg gctaactgcg cattcaccgc atcttgctgt
     9361 tgacgcagaa agtggttggt ggaggtcagg gccaactcga tttcatgttt aatctcgccg
     9421 ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt cttcgacctg atggatattt
     9481 tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag gtattcccgc
     9541 gcctgctgcg cgtaattcaa cagtttatgc gcggcagatc gcagagcata caacgaggcg
     9601 ttagcgtaag cggcatgaag caacgcctga attggctggg cgaacagcga atcttcccac
     9661 aactgatcgg cagcatggcg aatatgttcg aggtccgcca gatcggcatg acgccagcgc
     9721 ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt gttgctctgg cggtggtaac
     9781 ttaccgttgt tggctaactc atggcgcgcc cgattcgcca ggtagcccca catcgacgac
     9841 accggaaata tctgctgtgg cgtaatacag cctttcatca gcgttccgga aatcagtgcc
     9901 cgcacctggt cggcgtcgtc actgttacga tcctgttgat cgaacttatt gaccagcaca
     9961 tacaacggca ccgattgccc caccgccaaa attgcctcac ggacctcttc atcggagatc
    10021 gatttcagtt gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc cagctgctgg
    10081 ttaagcattt tttgcagatg cggttgcccg gcttcttttg cagatgcggt tgcccggctt
    10141 catttggccc gggggtatcc agtaacgtca actgaccggg ataactctcc agccccgcca
    10201 gatggacaaa ctccacttca atcacgggaa tatgctcaat ggcggcgtaa gcagaaaaag
    10261 gaaaatcgac gtccagcgcc ttcgccagtc gcaccaaatc attcaaactt ttcagacaat
    10321 gaaaaatagg ttgagcaccc agataatatt tttcgaaagc gatgccattt tcaatccgct
    10381 gcataagcgc acgcatatct ttatctattt ccagcacatc ggtcagatgc ttaatatcgc
    10441 aatcacgcag gcgctgttgt aattgttgaa ttaaatggtc gattggcgcg acatgtgaaa
    10501 aatgcagtac cggttctttt tgcccgggcg tatggcgaat aagcgttggc agcgcagtca
    10561 ttgggcgatt gcgattaggc agaacccccg taccaacaat ggcattaatg gtggttgatt
    10621 tccctgcttt catagtaccg acaatcgcca gcaccatttc cagtcgggaa attttacgca
    10681 actcattatt cagcatcgcg tgacgttcgg cgatattagg ctgactccag ggtaaagcca
    10741 gttgtggcgc atcgtctccg ggtacagaga gaggcatttt ttccagtaac tgcaactgtt
    10801 ggcgagaaag ctgtaacagg cgttcagcct cctgacttaa ctcatacagg gtctgtgtgt
    10861 acatagaaaa tttttcctta aagcaaattt tattactttt atttagccag attgtttttg
    10921 agttctgttt tcggctttta taattactgc aagaaataat tttatattta gagtgttgtt
    10981 ttttattaga ataaataacg tcttccgata cgtttaaaac gtcagaaaga taaaaaatat
    11041 catttgaatt aaaaaaagaa catgtagagc attaacctta tcttaaataa taaatggagg
    11101 caaaaagatt attttctttt tgcgtttcct ttcaaatgaa aacgatcgtc gtctaaaatc
    11161 agcagtaccc ccgacaaact cagggatttt gtgtataatt gcggcctttt tcggcaatct
    11221 gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa atccacatgt cattaccaca
    11281 ctgcccaaaa tgcaactccg aatacaccta cgaagataac ggcatgtaca tctgcccgga
    11341 atgtgcctac gaatggaacg acgcagaacc tgcacaggaa agcgacgagc tgatcgttaa
    11401 agatgctaac ggcaatctgc tggctgacgg cgacagcgtg accatcatta aagatctgaa
    11461 ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa gtgaaaaaca tccgcctggt
    11521 tgaaggcgac cataacatcg attgcaaaat cgacggtttt ggtccgatga aactgaaatc
    11581 tgagtttgtg aaaaagaact gattgtattg tgatcggtag gccggataag gcgtttacgc
    11641 cgcatccggc aacggtggcg actgcctgat gcgacgctga ccgcgtctta tcaggcttcc
    11701 ctctcatctg tataaatttc gaactacact taacgggctt ctcttaactg aggtcaccat
    11761 catgccgtta agtccctacc tctcttttgc cggtaactgt tccgacgcga ttgcctatta
    11821 tcaacgtacg ttgggcgcgg aactgctcta taaaatcagc ttcggcgaaa tgccgaaatc
    11881 agcgcaggac agcgccgaaa actgcccttc cggaatgcaa tttcccgata ccgccatcgc
    11941 tcatgccaac gtgcgcattg caggaagcga catcatgatg agcgatgcta taccgtcagg
    12001 aaaagccagc tactccggct ttacgctggt gctcgattcg caacaggtcg aagaaggaaa
    12061 acgctggttt gacaaccttg ccgctaacgg aaaaatcgaa atggcctggc aggaaacttt
    12121 ctgggcgcat ggcttcggca aagtcaccga taaatttggc gtaccgtgga tgattaatgt
    12181 cgtcaaacaa cagccaacgc aataacccgc cgggaggccc accctcccgc actgtcatcg
    12241 aattcccgtt aactcttcat ctgttagtca cttttaatta accaaatcgt cacaataatc
    12301 cgccacgatg gagccacttt tttagggagg ctgcatcatg caaacgatta tccgtgtcga
    12361 gaagctcgcc aaaaccttca atcagcatca ggcgctgcac gcggttgatc tgaacattca
    12421 tcatggtgaa atggtggctc tgcttgggcc gtcgggttcc ggaaaatcca cccttttacg
    12481 tcacttaagc ggtttgatta ccggcgataa atctgtcggt agccatatcg agctgctggg
    12541 ccgcactgtc cagcgcgaag gccgtctggc ccgcgatatc cgtaaaagcc gcgcccatac
    12601 cggctacatc tttcaacaat tcaacctggt gaatcgcctg agcgtactgg agaacgtgct
    12661 gattggcgcg ctcggcagca cgccattctg gcgaacctgt tttagctact tcacccgcga
    12721 gcagaaacag cgcgcgttac aggcgctgac ccgcgttggc atggtgcact ttgcccatca
    12781 acgcgtttcc acactctccg gcggccagca acagcgtgtg gcgattgccc gcgcgctgat
    12841 gcagcaggcg aaggtcattc tcgctgacga acgcatcgtc gccctgcgcc aggggcacgt
    12901 cttctacgac ggctgcagcc aacagtttga taacgaacgt tttgaccatc tctaccgcag
    12961 cattaaccgc gtcgaagaga acgcgaaagc tgcctgacat ccccatcatt gaggaaaacg
    13021 aatgaacgct aagataattg cctcgctggc cttcaccagc atgttcagcc tcagcaccct
    13081 gttaaatccg gcatacgccg aagagcagga aaaggcgctg aatttcggca ttatttcaac
    13141 ggaatcacag caaaacctga aaccgcaatg gacgccgttc ttacaggata tggagaagaa
    13201 gctgggcgta aaggtcaacg ccttctttgc cccggactac gcgggcatta tccaggggat
    13261 gcgcttcaat aaagtggata tcgcctggta cggcaacctg tcggcgatgg aagcggtgga
    13321 tcgcgccaac ggccaggtct tcgcccagac ggtcgcggcg gatggatcgc cgggttactg
    13381 gagcgtgttg atcgtcaaca aagatagccc gatcaacaac ctgaacgatc tgctggcgaa
    13441 gcggaaagat ctcaccttcg gcaatggcga tcctaactct acctccggct tcctcgtccc
    13501 cggttactac gtcttcgcta aaaacaatat ctccgccagc gacttcaagc gcaccgtcaa
    13561 cgccgggcat gaaaccaatg cgctggccgt cgccaacaag caagtggatg tggcgaccaa
    13621 taacaccgaa aacctcgaca agctgaaaac ctccgcacca gagaagctca aagaactgaa
    13681 ggtgatctgg aaatcgccgc tgatcccagg cgatccgatc gtctggcgca aaaatctgtc
    13741 cgaaaccacc aaagacaaga tctacgactt ctttatgaac tacggcaaaa cgccggaaga
    13801 gaaagcggtg ctggaacgcc tgggctgggc gccgttccgc gcctccagcg acctgcaact
    13861 ggtgccgatt cgccagctcg cgctgtttaa agagatgcaa agcgtgaagg acaacaaagg
    13921 gctgaacgaa caggacaagc tggcaaaaac caccgccatt caggcgcaac tggatgattt
    13981 ggaccgcctg aacaacgcgt taagcgcaat gagttcggtg agtaaagcgg tgcagtaaat
    14041 cgtaggtcgg ataagacgcc ccggcgtcgc atccgacaat gtgcagacgt tgatgccgga
    14101 tgcggtgcaa gcaccttatc cggcctacag accggagcca aacatgcaaa ccatcaccat
    14161 cgccccaccc aagcgcagct ggttctcgct tctgagctgg gccgttgtta tcgccgtgct
    14221 ggtcgtctcg tggcagggcg cggaaatggc accgcttacg ctgattaaag atggcggcaa
    14281 catggcgacg ttcgccgccg acttcttccc gcccgatttc agccagtggc aggattacct
    14341 caccgaaatg gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cggtggttct
    14401 ctccatcccc ttcggcctga tgagcgctga aaacctggta ccgtggtggg tctaccaacc
    14461 tgtacgccgc ctgatggacg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt
    14521 cgtggtcgcc gtcggcctcg gcccgttcgc tggcgtgctg gcgctgttta tccacaccac
    14581 cggcgtactt tccaaactgc tttccgaagc ggtagaagcg attgaacccg gcccggtgga
    14641 gggcattcgc gctaccggtg ccaacaagct ggaagagatc ctctacggcg tgctgccaca
    14701 ggtgatgccg ctgctgatct cctactccct ctatcgtttc gaatccaacg tccgctcggc
    14761 gaccgtcgtc ggcatggtcg gtgcaggcgg gatcggcgtc accctgtggg aagcgattcg
    14821 cggtttccag ttccagcaaa cctgcgccct gatggtgctc atcatcgtca cggtcagcct
    14881 gctggatttc ctctctcaac ggttgcgtaa gcactttatc tgataagcga ggcattgatg
    14941 gctatgcact tgtctacaca tccgaccagc tacccaacac gctatcagga gatagccgca
    15001 aaacttgagc aggagcttcg tcaacactac cgctgcggcg actatcttcc cgccgagcag
    15061 caactggcgg cgcgctttga ggtgaaccgc cacaccctgc gccgcgccat cgaccagctg
    15121 gtggaaaaag gctgggtaca gcgccgtcag ggcgtcggcg tactggtgct gatgcgcccg
    15181 ttcgattacc cgctcaacgc ccaggcgcgt tttagccaga acctgctgga tcagggcagc
    15241 catcccacca gcgaaaaact gctatcggtg ctgcgccccg cctccggtca cgtcgctgac
    15301 gcgctgggga taaccgaggg ggagaacgtc atccacctgc gcaccctgcg tcgggtcaac
    15361 ggcgtcgcac tctgtttaat cgatcactac ttcgcagatc tcaccctctg gccaacgctt
    15421 caatgctttg acagcggttc gctacacgat tttctgcgcg agcaaacggg gattacgcta
    15481 cgacgcagtc agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg ccagcgtctt
    15541 gaaattccga atatgtcacc gctgctgtgc gtgcgcaccc ttaaccaccg tgacggtgaa
    15601 agcagcccgg cggaatactc cgtcagcctg acacgcgccg acatgattga attcactatg
    15661 gagcactgaa tgcacgcaga taccgcgacc cgccagcact ggatgtccgt gctggcgcac
    15721 agccaaccgg ctgaactggc agcccgcctg aacgcgctga acatcaacgc cgattatgag
    15781 gtgatccgcg ccgctgaaac tggcctggtg cagattcagg cgcgaatggg cggcaccggc
    15841 gaacgctttt ttgctggcga cgccacgctc acccgcgccg ccgtgcgtct gactgacggc
    15901 acactcggct atagctgggt actggggcgc gacaaacagc acgccgaacg ttgtgcgctg
    15961 attgatgcgc tgatgcagca aaaccgccac tttcaaaact tatcagaaac ccttattacc
    16021 ccgctggacg ctgaccgtat ggcacgcatt gccgcgcgtc aggccgaagt gaacgccagc
    16081 cgggtcgact tctttacgat ggttcgcgga gacaacgcat gaccctggaa accgctttta
    16141 tgcttcccgt gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc atgagcgagc
    16201 cgggcgtgat tgtcgccctg catcagctca agcgcggctg gcagccgctg aatatcgcca
    16261 ccaccagcgt gctgctgacg ctggccgata acgacacgcc ggtgtggctt gccgcaccat
    16321 tgagcaacga tatcgtcaat cagagcctgc gttttcatac caacgcgccg ctggttaatc
    16381 agccgaaaca ggcgaccttc gcggtaacag atgaggcgat ctccagcgag cagctcaacg
    16441 ccctttccac cggcactgcc gttgcaccag aagcgggcgc aacgctgatt ttacaggtca
    16501 ccagcctgag cggtgggcgt atgttgcgcc tcaccggcgc aggtattgcc gaagaacgga
    16561 tgatcgcccc gcaactgcca aagtgcattc ttcatgaact taccgagcgc ccgcacccgt
    16621 tcccgctcgg catcgacctg atcctgacct gcggcgagcg cctgctggct attccgcgaa
    16681 ccactcatgt ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaaa aggcgatcga
    16741 cgccgcccac gccctgcaag agagccgacg ccggggcgat accgatttgc ctgaactaag
    16801 cgtcgcccag attgaacagc agcttaacct cgcggtagat cgcgtgatga ccgaaggcgg
    16861 tattgccgac cgcgagctgg ccgcgctggc gctgaaacag gccagcggcg ataacgtcga
    16921 agcgattttc ctgttgcgcg cctaccgtac cacgctggcg aagctggcgg tcagtgagcc
    16981 gctcgacacc accgggatgc gtctcgaacg gcgtatttca gcaatttata aagacattcc
    17041 cggcggccaa ctgcttggcc ccacctacga ctacacccat cgcctgctcg atttcaccct
    17101 gctggcaaac ggcgaaacgc caacgctgac caccgctgac agcgaacagc aaccgtcgcc
    17161 acatgtcttc agcctgctgg cgcgtcaggg gctggcgaag tttgaagaat atagcggcgc
    17221 acaaccggac gacatcaccc gcacgccgcc ggtttatccc tgttcgcgtt cttcccgtct
    17281 acaacaatta atgcgcggtg acgaaggcta tttgttggcg ctggcctact ccacccaacg
    17341 cggttacggg cgcaatcacc cgttcgcagg cgaaattcgc agtggctata tcgatatatt
    17401 tattgtgccg gaagagctgg gatttgcggt gaacgtcggc gaactgttga tgaccgagtg
    17461 tgaaatggtc aacggtttta tcgacccgcc ggatgaacca ccgcatttta cacgcggcta
    17521 tgggctggtg ttcggcatga gcgagcgcaa agcaatggcg atggcgatgg ttgaccgtgc
    17581 gttgcaggca ccggaatacg gcgagcacgc aacaggtccg gcacaggatg aagagttcgt
    17641 gctggcccat gccgacaacg tcgaagccgc aggctttgtt tcgcatctca aactcccgca
    17701 ctacgtcgat ttccaggccg aactggagct actcaaacgt ctgcaacagg agcagaacca
    17761 tggctaatct gagcggctac aactttgctt acctcgacga gcagaccaaa cgcatgatcc
    17821 gccgcgctat cttaaaagcg gtggcaatcc ccggttatca ggtgccgttt ggcggacgcg
    17881 agatgccgat gccctacggc tggggaaccg gcggcattca gctcaccgcc agcgtgattg
    17941 gcgaaagcga cgtgttgaag gtgattgacc agggcgcaga cgataccacc aacgccgtgt
    18001 cgattcgtaa cttctttaag cgcgtgaccg gggtgaacac caccgagcgc acggacgatg
    18061 cgacacttat ccagacgcgt caccgcatac ccgaaacgcc gctgaccgaa gatcagatca
    18121 ttatcttcca ggtgccgatc cccgaaccgc tgcgctttat tgagccgcga gagacggaaa
    18181 cgcgcaccat gcacgcgctg gaggagtacg gcgtgatgca ggtgaaactg tatgaagata
    18241 tcgcccgctt cggtcatatc gccaccacct acgcctatcc ggtgaaggtg aacgggcgct
    18301 acgtaatgga cccgtcgccg atcccgaaat tcgataaccc gaaaatggac atgatgtccg
    18361 ccctgcaact gtttggcgcg gggcgcgaga agcgcatcta tgccgtgcca ccgtttaccc
    18421 gcgtggaaag cctcgatttc gatgatcatc cgttcaccgt ccagcagtgg gatgaaccat
    18481 gcgccatctg cggatcgacc cacagttacc tcgacgaagt ggtgctggat gacgccggaa
    18541 accgcatgtt tgtctgctcc gataccgatt attgccgcca acagaacgag gccaaaagcc
    18601 aatgaatcaa ccgttacttt cggtcaataa cctgacccac ctttatgccc cgggcaaagg
    18661 cttcagcgat gtctcttttg atttatggcc gggagaagtt ctgggcattg ttggggagtc
    18721 cggttccggg aagaccacgc tactgaaatc aatttccgcc cgcctgacac cacagcaagg
    18781 ggaaattcgc tacgagaacc gttcgctgta cgcgatgagc gaggccgacc gccgtcgcct
    18841 gctgcgcacc gaatggggtg tggtgcacca gcatccgctt gacggcctgc gccgccaggt
    18901 gtcggcaggc ggcaatatcg gcgaacggtt gatggcaacc ggagcgcgtc attacggcga
    18961 tattcgcgcc accgcgcaga aatggctgga agaggtggag attcccgcca accggatcga
    19021 cgacctgccg accacctttt ccggcggcat gcagcaacgt ttgcagattg cccgcaacct
    19081 ggtgacgcat ccgaagctgg tgtttatgga tgaacccact ggcgggctgg atgtgtcggt
    19141 acaggcgcgc ctgctggacc tgctccgcgg tctggtgatg gagcttaacc tggcggtggt
    19201 gattgtcacc cacgatttag gcgtcgcccg tctgctggca gaccgtttgc tggtaatgaa
    19261 gcaagggaaa gtggtggaga gtgggttgac cgaccgggtg ctcgacgacc cgcaccatcc
    19321 gtatacccag ttgctggtgt catcggtttt gcagaattga gctgatgccg gatgcggcgt
    19381 aaacgcctta tccggcctac aaatgcgctc cccgtaggtc ggataagacg catcagcgtc
    19441 gcatccgaca cccgaaccac gaggcgaaaa atgattaacg tacaaaacgt cagtaaaacc
    19501 ttcatcctgc accagcaaaa cggcgtgcgc ctgcctgtat tacagggcgc ctcactcacc
    19561 gtcaacgccg gcgaatgcgt ggtactccac ggccattccg gcagtggaaa atcaactctg
    19621 ctacgctcgc tgtatgccaa ctatctaccc gacgaagggc aaatccagat caaacacggt
    19681 gatgaatggg tcgacctggt caccgcgcca gcgcgcaaag tggtggagat ccgcaaaacc
    19741 actgtcggct gggtaagcca gtttctgcgc gtcatcccac gtatttcggc actggaagtg
    19801 gtgatgcagc cgctgctcga taccggcgta ccgcgtgaag cctgcgccgc taaagccgcg
    19861 cgtctcctca cccacctgaa cgtgcctgaa cgcctgtggc accttgcgcc atcgaccttc
    19921 tccggtggcg aacagcagcg tgtcaacatc gcccgtggct ttatcgtcga ctaccccatt
    19981 ctgctgctcg acgaacctac cgcctcgctg gacgctaaaa acagcgccgc cgtagtggaa
    20041 ctgatccgcg aagccaaagc ccgcggcgcg gccatcgtcg gtatcttcca tgacgaaacc
    20101 gtgcgcgagc aagtcgccga ccgcctgcac ccaatgggag tctcttcatg attatcaata
    20161 acgtcaaact ggtgctggaa aacgaggtgg tacacggttc tctggagatg caggacggcg
    20221 aaatccgcgc ctttgccgaa agccagagtc acctgtcaga agcaatggac ggcgaaggcg
    20281 gctggctgct accggggctg attgagctgc ataccgataa tctggataaa ttcttcaccc
    20341 cgcgcccgaa agttgactgg cctgcccact cggcgatgag cagccacgac gcgctgatgg
    20401 tggcgagcgg catcaccacc gtgctggacg ccgtggcgat tggtgatgta cgcgacggcg
    20461 gcgatcggct ggagaatctg gagaagatga tcaacgccat cgaagagacg cagaaacgcg
    20521 gcgtcaaccg tgccgagcac cgtctgcacc tacgctgcga actgccgcat cacaccacgc
    20581 tgccgctgtt tgaaaaactg gtacagcgcg aaccggtaac gctggtgtcg ctgatggatc
    20641 actcaccggg ccagcgccag ttcgccaacc gcgaaaaata tcgtgaatat tatcagggca
    20701 aatactcgct caccgatgcg cagatgcaac agtacgaaga ggagcaactg gcgctcgccg
    20761 cacgctggtc acagccgaat cgcgaaacta tcgccgcaat gtgccgcgcg cgacacattg
    20821 cgctcgccag ccacgatgac gccacgcacg cccacgtagc ccaatcccat cagcttggca
    20881 gcgtgatcgc cgaatttcct accacattcg aagcggcaga agcctcgcgc aagcacggca
    20941 tgaacgtact gatgggtgcg ccgaatatcg tgcgcggcgg ttcgcactcc ggcaacgtgg
    21001 cggccagtga actggcacag cttggcctgc tggatatcct ctcttccgac tactaccccg
    21061 ccagcctgct cgatgcggca tttcgcgtcg ccgatgacga gagcaaccgc tttacgctgc
    21121 cgcaggcggt gaagctggtg actaaaaatc cggcgcaggc gcttaatctt caggatcgcg
    21181 gggtgattgg cgagggcaaa cgcgccgacc tggtgctggc gcatcgcaag ggcaaccaca
    21241 ttcatatcga ccacgtctgg cgtcagggta aaagggtgtt ctgatgacgg gaaaactgat
    21301 ttggttaatg ggggcctctg gctccggaaa agacagtctg ttgacggaac tccgccagcg
    21361 ggaacaaact cagctactgg tagcgcatcg ctacatcacg cgcgccgcca gcgccggaag
    21421 tgaaaaccat atcgccctga gcgagcagga gttttttacc cgcgcggggc agaaccttct
    21481 ggccttaagc tggcacgcta acggtctgta ttatggcgtc ggcgtcgaga ttgacctctg
    21541 gctgcacgct ggatttgacg tggtggtcaa cggctcacgc gcccatctgc cgcaggcgcg
    21601 ggcgcgttat caatcggcgc tgctgcccgt ttgtttacag gtttcgccag agatcctgcg
    21661 ccagcgcctg gaaaaccgtg gccgtgaaaa cgccagtgaa attaacgccc ggctggcgcg
    21721 cgctgcccgc tatactcctc aggattgtct tacgctcaat aatgacggca gcctgcgcca
    21781 gtcggtcgac aagctgctga cgctgattca tcagaaggag aaacaccatg cctgcttgtg
    21841 agcttcgccc ggctacgcag tacgacaccg acgcggttta cgcgctgatt tgtgagctaa
    21901 aacaagcgga gtttgaccac caggcgtttc gcgtgggctt taacgccaac ctgcgtgacc
    21961 cgaatatgcg ctaccatctg gcgctgttgg atggcgaggt tgtcggcatg atcggcctgc
    22021 atttgcagtt tcatctgcat catgtcaact ggatcggcga aattcaggaa ctggtggtga
    22081 tgccgcaggc gcgcggtctg aatgtcggca gtaaattact ggcatgggca gaagaagaag
    22141 cccgccaggc cggggccgaa atgaccgagc tttccaccaa cgtgaagcgc cacgacgcgc
    22201 accgatttta cctgcgcgaa ggctacgagc agagccactt ccgcttcacc aaggcgctgt
    22261 aatatgagct tgactctcac gctcaccggc accggcggcg cacagggcgt tccggcatgg
    22321 ggctgtgatt gcgtggcctg cgccagagcg cgacgctcac cgcagtatcg ccgccagcca
    22381 tgcagcggcg tagtgaagtt taacgaggcg atcaccctga tcgacgccgg gcttcacgat
    22441 ctcgccgatc gctggtctcc cggatcgttc cagcagtttt tactgacgca ctaccatatg
    22501 gatcacgtcc aggggctgtt cccgctgcgc tggggcgtgg gcgacacaat cccggtctac
    22561 ggtccaccgg atgagcaggg ctgcgacgat ctgtttaaac atccgggcct gcttgatttc
    22621 agccacacgg tggagccgtt tgtggtgttt gatttgcagg ggttgcaggt cacgccgctg
    22681 ccgcttaacc attcgaaact cactttcggc tatttactgg aaacggcgca cagccgggtg
    22741 gcgtggctat ctgacaccgc aggcctgccg gaaaaaacgc tgaaattttt acgcaataat
    22801 cagccgcagg taatggtgat tgattgcagc cacccgccgc gcgaggatgc accgcgtaat
    22861 cactgtgatt taaataccgt gcttgcgctg aatcaggtta tccgctcgcc gcgggttatt
    22921 ctgacccata tcagccatca gtttgatgcg tggctgatgg aaaacgcgct accatcgggg
    22981 tttgaggcgg ggtttgatgg gatggagatt gaggtggcgt gagggaaaat acccgcgccg
    23041 gcccctatat caacgcccat actcatcttc cattctccgc tcttcatctt ccagttgccg
    23101 acgctcctga tcaagctggc gctggcgatc gtccagctgc ctgcggcgat cttcaaactg
    23161 gcggcggcga tcgttatatt gcttgcgacg atcgtcgctc acctggcgtt gccaaccgtc
    23221 gtcgcgcgaa tcttcatagt ctcgcccacg gtcagggtta taagcgtcgt taatcgcctg
    23281 ctgaatattg ccaatggtgt cgtcgataat atcggcctgg gccggaacgg tggacagcgt
    23341 gagcagggtg aataaaagaa atagcggaaa gcgtttcatt agccaacctc aaaaagaaac
    23401 tctatccaca ttagtcatta ctcatccatg caagtagtgg atgaatctca atttctccgc
    23461 tgctctattg ccgtaatcgc ctccacgcgt tgttgatgac gaccgccttc gtactgtgcg
    23521 cccagccacg catcgacaat catttttgcc agttcgaggc caactactcg tgaaccaaaa
    23581 gccagcacgt tggtgtcgtt atgctgccgc gaaagttgcg cggaataagg ttcgctacaa
    23641 acgaccgcgc gaattccggc aaacttgttc gccgctatcg aaataccgac gccagtgcca
    23701 caaatcaaaa tcccgccatc agcctctccg ccagcaacgg ccagtgcgac ttgactggcg
    23761 taatgcggat aatcagtacg ctctgacgac caggttcctt tatcaatcac ttcaacgcca
    23821 cgctcaacta aatgtgccac tatttcatgt tttaaaatga aaccgacatg atcacagcca
    23881 aatgcaatct ttttcattca tcttccccgc ccgtgcaaaa cttcacaatc aaaaaacatt
    23941 tgtgatgtaa atgaattaaa aacatccaaa atcgatgaaa tccgccttcc cgatctgttt
    24001 tacagagtta catctggcag cgcaatagcg ttaaagacac tcactatcca gcaacatcta
    24061 tcatctaata aaccaggaag acaaataaca tcatgttttt
//
LOCUS       DADTFG010000071        24080 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.071, whole genome shotgun sequence.
ACCESSION   DADTFG010000071 DADTFG010000000
VERSION     DADTFG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.071"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            14..943
                     /gene="aaeR"
                     /locus_tag="J1138_002593"
     CDS             14..943
                     /gene="aaeR"
                     /locus_tag="J1138_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBA8454370.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1071..2516)
                     /gene="tldD"
                     /locus_tag="J1138_002594"
     CDS             complement(1071..2516)
                     /gene="tldD"
                     /locus_tag="J1138_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBA8454371.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2672..6472)
                     /locus_tag="J1138_002595"
     CDS             complement(2672..6472)
                     /locus_tag="J1138_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HBA8454372.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6540..8009)
                     /gene="rng"
                     /locus_tag="J1138_002596"
     CDS             complement(6540..8009)
                     /gene="rng"
                     /locus_tag="J1138_002596"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBA8454373.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(7999..8592)
                     /gene="yhdE"
                     /locus_tag="J1138_002597"
     CDS             complement(7999..8592)
                     /gene="yhdE"
                     /locus_tag="J1138_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBA8454374.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8601..9089)
                     /gene="mreD"
                     /locus_tag="J1138_002598"
     CDS             complement(8601..9089)
                     /gene="mreD"
                     /locus_tag="J1138_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBA8454375.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9089..10192)
                     /gene="mreC"
                     /locus_tag="J1138_002599"
     CDS             complement(9089..10192)
                     /gene="mreC"
                     /locus_tag="J1138_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBA8454376.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10258..11301)
                     /locus_tag="J1138_002600"
     CDS             complement(10258..11301)
                     /locus_tag="J1138_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBA8454377.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11606..13546)
                     /gene="csrD"
                     /locus_tag="J1138_002601"
     CDS             complement(11606..13546)
                     /gene="csrD"
                     /locus_tag="J1138_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBA8454378.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRHIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13698..14672
                     /locus_tag="J1138_002602"
     CDS             13698..14672
                     /locus_tag="J1138_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA8454379.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            14858..14929
                     /locus_tag="J1138_002603"
                     /pseudo
     CDS             14858..14929
                     /locus_tag="J1138_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            15650..16120
                     /gene="accB"
                     /locus_tag="J1138_002604"
                     /gene_synonym="fabE"
     CDS             15650..16120
                     /gene="accB"
                     /locus_tag="J1138_002604"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBA8454380.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16131..17480
                     /gene="accC"
                     /locus_tag="J1138_002605"
     CDS             16131..17480
                     /gene="accC"
                     /locus_tag="J1138_002605"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBA8454381.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17572..18618)
                     /locus_tag="J1138_002606"
     CDS             complement(17572..18618)
                     /locus_tag="J1138_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBA8454382.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDDGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLASHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDIMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFLDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(18615..19577)
                     /locus_tag="J1138_002607"
     CDS             complement(18615..19577)
                     /locus_tag="J1138_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA8454383.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19599..20588)
                     /locus_tag="J1138_002608"
     CDS             complement(19599..20588)
                     /locus_tag="J1138_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8454384.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(20589..22088)
                     /locus_tag="J1138_002609"
     CDS             complement(20589..22088)
                     /locus_tag="J1138_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA8454385.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22149..23039)
                     /locus_tag="J1138_002610"
     CDS             complement(22149..23039)
                     /locus_tag="J1138_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBA8454386.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(23075..23929)
                     /locus_tag="J1138_002611"
     CDS             complement(23075..23929)
                     /locus_tag="J1138_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HBA8454387.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVIRIIG
                     VCGSEGKI"
BASE COUNT         6025 a         6476 c         5706 g         5873 t
ORIGIN      
        1 gtcagagtga ataatggaac gactaaaacg catgtcggtg tttgccaaag tagttgaatt
       61 tggctctttt accgccgccg ccagacagct acagatgagc gtttcgtcca tcagtcagac
      121 ggtgtcaaaa ctggaagatg agttacaggt aaagctgtta aaccgtagta cacgcagcat
      181 tggcctgact gaagccggca gaatttacta ccagggctgc cgtcggatgc tccatgaagt
      241 gcaggatgtt catgagcaac tgtatgcctt caataatacc cccatcggga cgctacgcat
      301 tggctgttct tcaactatgg cacaaaatgt tctcgccggg ctgacagcaa aaatgctgaa
      361 agaataccca ggtttgagcg tcaatctggt taccggaatt cctgcccccg acctgattgc
      421 cgacggtctg gatgtggtga tccgcgtcgg cgcgttgcaa gattccagcc tgttttcccg
      481 ccgtctgggc gcgatgccaa tggtggtgtg cgccgcaaaa agttatctca cacaatacgg
      541 cataccggaa aaacccgccg atttaagtag tcactcatgg cttgaataca gcgtgcgacc
      601 cgacaatgaa tttgaactga tcgcaccgga agggatctcg actcgcctga tcccgcaagg
      661 gagatttgtg actaatgatc cgatgacgct ggtccgctgg ctgacggcgg gtgccgggat
      721 cgcctacgtg ccgttgatgt gggtgatcaa cgagatcaat cgcggggaac tggagatcct
      781 gctaccacgt taccagtcag atccacgccc ggtttatgcg ttatataccg aaaaagataa
      841 gctgccgctg aaggtacaag tcgtgatcaa ctcgctaacg gattattttg ttgaggtcgg
      901 taaattgttt caggagatgc acgggcgcgg gaaagagaag taattaatat tgtactgtta
      961 cgttgtacaa acctgtgcca acgggttccc ctcaccctaa ccccagaggg gcgaggggac
     1021 cgttcgtaca cggagaagga ttaattatcc ttctgaaaat actgaaatta ttacgcagta
     1081 ccgccaacag tcaggttatc aactttcagc gttggctggc ccacgccaac cggcaaactt
     1141 tgcccttctt taccgcagac acccacgccg ttatccagtt tcaggtcgtt gccaaccatc
     1201 gaaatctgct gcatggtttc gataccggaa ccaatcaacg ttgcgccttt caccggctta
     1261 gttactttac cgttttcaat aagatacgct tctgaggtgg agaaaacgaa tttgccggag
     1321 gtgatatcca cctggccgcc accaaagttc ggtgcataga taccgtactc aacggattca
     1381 ataatttcct gcggggtcga tttacccggc agcatatagg tgttggtcat acgcggcatg
     1441 ggcagatgag cgtaggattc acggcgaccg ttgccagtcg gcgtcatccc catcaaacgc
     1501 gcgttgagtt tatcctgcat gtagcctttc agaatgccgt tctcaatcag cacgttgtac
     1561 tggcctggcg taccttcgtc atcaatcgcc accgaaccac ggcgatcgac catcgtgcca
     1621 tcatcaacca cggtacacag ttctgaagcc accaactccc cgacctgtcc actaaatact
     1681 gaagtgccac ggcggttgaa gtcgccttcc agaccgtgac ccaccgcttc atgcaacagc
     1741 acgcccggcc aacctgcgcc aagtactacc ggcatggtgc ccgctggtgc agcaacggca
     1801 gaaagattga ccagcgccat acgcactgct tcttttgccc acgcatccgc acggacttcg
     1861 ccgtcgagat cagcaaggaa gaactcataa ccaaaacgac cgccgccacc actggcaccg
     1921 cgttcgcgtt tgccatcttc ttcgaccaaa acgctcacgg aaagacgcac aagcggacgg
     1981 acatccgccg ctagtgtgcc gtcggtggcc gcaaccagaa ttaattcata gacaccactg
     2041 aggctggcag tcacttcctg tacgcgcttg tccgcttcgc gggcaacctt atcgacgcga
     2101 cgcaggatat ccaacttctc ttcacggctc atgctttgca gcggatctac cgaggtatat
     2161 aacgggctat gctctaccgc gcccagcgtc tgtactttgc catcaccact atcacggacg
     2221 atggtgcgcg ccgcttgtgc actctgttcc agcgccagca ggctgatttg gtcagcataa
     2281 gcaaatccgg ttttttcacc actgattgca cgcacgccaa cgccctgatc gatgttgtaa
     2341 gaaccatctt taataatacg gtcttctaaa acccaggatt cgtgatagct cgactgaaaa
     2401 tagagatcgc cataatcaag gcgacgttcg gccagttgac cgaggatcgc gaacaagtcc
     2461 tgatgtttca ggccgttcgc cgctagcaat tgttcactta ccaggttcag actcatcgtt
     2521 tttgctactc gttagttact gcagtagagg attttttacg gctgccggat gcggcgtgaa
     2581 cgccctgtcc ggcctacggt tatgttctgg ctcgttggcc tttggcaacg attatcatat
     2641 gagattgggg caattacgcg ccctcgtcaa atcattgcgc tttttctttg cgcggttgtc
     2701 gcaacacttc gttgatttgc ggatcgtcca gcggacccga aatgtgatag cgcaaaatgg
     2761 agactttgct ccacagcggt cccagcactt tactggcggc aaacactgcc gcgccaacaa
     2821 tggggttaac cgcaaaggcc gcagccacgc cgaccgtcgc agaaatctct ggtgcgacaa
     2881 ccgcttccat attcaggtcg cgacgtacca gatttaccga ccctttcata gcgatgtccg
     2941 cttccaggcc atccaccagc gtgtcgtcgg tgtgcataac gccgtcttta atccacgcgg
     3001 tgctgcgaat ggagtcaaaa tagaaccctt cgccaaaagt gtctctgaaa tcaaaacgca
     3061 gcttacgcat cagggcatct acgctcaata agcgcagcaa ttgcccggca tgtccggtat
     3121 tgatttcggt aatttcgcct ttacccagtt gagtatgaat gatgccattc aacgtcgcct
     3181 catctggctg ccagggtgct ttgcgccagt gtaaatcgta atccacatta aatgacgact
     3241 ggcgtatggg cgtcgtgaca ccaaaaaatt ctgcggcggc atcaattttc tgcccgcgca
     3301 gttttccttt cagcgaggta cgttcattcc ccggattgtt aacccattca ccatcggcag
     3361 tgagccgcga aaaaccagta tcaatcagtc cattagtcag cgttaacgta tcgccagaaa
     3421 tggtgatatc actgtcaata cgaccgaatt tttgccccca gaaccagcac tctgcgcatc
     3481 gtatttgtgc gtctggccag ccacggaagt taatgcgttc cgttgtcggg aaaggtgatg
     3541 actgtgttga atcaccacga gttttcgcca cgcttgggtt gtaataaaga tatttgatat
     3601 tcgccagcca cggtgcgtta ttacgcatcg ccagcgtggc gttgatttca cgcccttgcg
     3661 cctcaaccag ggtgccattt gccgtcggtt gcgaaacaat actcaggtta ttccattgct
     3721 gatttcccag cgacaacata ggcgtacgta acgttatgtg ttgtgggaaa ctcgctgcac
     3781 caccgacact ctccgccgcg cctttctgga acagggccag ccactcggca ccattcatcg
     3841 gcggcatatt gagttcaacg ccactttgtt ccggcaacgg cgggagcgtt ttactgtctg
     3901 ccgcccaaat agcacgatcg agcgtcagct tttgaccgag caaccagcgg ctattgaaat
     3961 ggttatccgc accagcctgt ccggttaatt caaagctgtt gagattgcca tcaaccttaa
     4021 cgtttaccgg tagtggttca cccgcaggtt tggctaacgg tgaaggtaag tgactgctca
     4081 cattcttcaa atcgccgttc agctctacgt tatacgtcgc gccagcatga taaggcagct
     4141 caatgcccac tttaccatcc cacgccacgc tgccacttaa tgcttcgttc accgctgcag
     4201 gcagaacgcc ggttttcgct ggttgccagt taccgttgag attcaccgcg acctggtagg
     4261 cttttgcccc ttctttggtg gaaaaatcca cgttcaacgg ctgattaaac cagcttgctg
     4321 tcagtggttc actttgcaga tcgccattga taaagctgaa tttaccgctc aaatttttca
     4381 gggtgctgtc gagtggtttg ataaacagac tgttattacg cagcgtcact tcccctttcg
     4441 cggttaccaa ctcgccgttc agcgggatat caagatgtaa gcgagcattc acatcgccgt
     4501 cgagttggag ttcttgcagg gtcgcaccca gagaatcttt cagcggtgtc tcatcaaagt
     4561 aagggccaac ggctttaccc ggacctttaa tgtcagcgtc aatcagcagt ttttctttcg
     4621 agtagtcagg gatcactgcg gtgagattac tcgcgcgcac gccgcccaga ttaacgccat
     4681 cggttttcat ccataaaccg tcgttaataa agtccagttc aatatcaagg ttagttaatg
     4741 caggccagtc cggctggaag gcaaacttcg cgttgcgcag cggtaccagc acttcaaact
     4801 gaccttcgtt gtgtttatag gggaagagtt gcggattacc accataaacc agcgtcgcgt
     4861 tatccgcttc accgccctga atagcgccac ttaagtaatc aaccaggtct ttacccatca
     4921 agttttccgg gaaatagcgc caggcttgtg aaccatcatc ggtactgatg ccagccagaa
     4981 tacccagcca gggttcatcg ttagcaggtt gcaggtaacg aaaaccgccg cgcgcatgga
     5041 cggctttggc tttaacgtca atattacgcc catccagctg gaaacctttg tcattgttca
     5101 gccagcttat cgttgcctgg ccgtcggcga tttccagtgg cgcacggaat accgtttcgt
     5161 aaggcatctt tgcctgcttc atcgacgcgg taagcaaacc attttctacg ctgccggaaa
     5221 gcgtcccgga gaagtgttcc gcacccggta ataatttcca ttgcttccag gccagatcgc
     5281 tccacgatgc ctgaaaacgg gtcttgtctg cagcctgaag cgggatatcc agcgccagag
     5341 tgttaatctt gccgctcggt tgtgtggagc gccaaacatc acccagtgca ggtgaaagtt
     5401 tcgcgaccag cgggcgtacg ccctccaggc ctgccagctc cagattactg gcacgaatcc
     5461 ggagttcgtc actgcgttta ttgtctttgc cgccaacgtc ctgttccggt atccaggcca
     5521 gcgtcaatgc tccgctcggc cagggtttgc cgtccatcgt gatccgtgta tcgggaatag
     5581 agaactgcca tcccggattt tcacgcgtaa tatgcgcagt cagattatcc accgacagcg
     5641 tatgtgtttc cttctcgccc aaccagctgg caccgccctg tttcagccag acgtcaccgc
     5701 cggttacatc gcctttgtcg atcgtcatcc agccttcaag ggagaactgt gccgtttcca
     5761 gcgcaatatt gtcctgcatc catttaccga gccacggctt caggtcgatg tcatccgcct
     5821 ggagccagac gcgaccattg cttaacaacc cctcatcatc gcgcaaatcc atacgcacct
     5881 gcatcacgcc gtgctgtccg gtaaggctgg agaggcttac caggccttcc gcacggtgtc
     5941 gacgtggatc gttcagccag gtgagctgtg ggatcgccag ctcggcgcgc tgaccggatg
     6001 gcgtcaggaa actgacttca ctgtcgcgga gatcgaaatg gtcaaattga cgaagaaaca
     6061 gatcgctgat gtgactggct tccaggctat cattaccacc gccgctggtg ataggagtgt
     6121 tggtgcgaaa acgcagctgc cagaaagtga ggtcgcgaaa ctgccagcgc atatgtaaca
     6181 ggctctgcca gacatccagc gccagagtaa cgcgtttaac cgaaaattcg ccgccatctt
     6241 ttagttctgc acggatgtcg tgcgcttcaa gcgtcggacc aaaattctgc cagctggctg
     6301 agagctgact ggcttctacc ggcatgccag tcgcggattc tattttgttg aggatttccg
     6361 gacgccaggc gtcaagatgc ggtaaagcaa tacgcaggcc gctaaccagc agggcagcga
     6421 tcacaacgag cgcggctcca gtaagcagta aaatccccgg caatcgcctc acccgtcact
     6481 ccttgtctgc taaaaatgtg actcaaaaac cctttgccgg atggcggccc agcatctgtt
     6541 tacatcatta cgacgtcaaa ctgctcctgg ttatagagcg gttcaatttg tactttaacc
     6601 tgtttgccaa cgaaaatttc cacttccgcc agcgagtgtg actcttcgcc tttcaaggct
     6661 tcggctactg ccggagaagc atagaccagg aaacggtcgg agtcgtaagc atggtggaca
     6721 cgaacaatct cgcgcatgat ttcatagcat accgtttcca cggttttcac cgttccgcga
     6781 ccgtggcagg ttgggcattc gttacacagt acgtgctcaa tgctttcgcg ggtgcgttta
     6841 cgcgtcatct ccaccagccc cagcgccgaa aaaccattaa cgctggtttt cacccgatct
     6901 ttgctcagcg cctgctccag cgagtgcagc acgcggcgac ggtgatcttc attattcata
     6961 tcgatgaaat caataatgat aatcccgccc agattgcgca accgtaactg gcgagcgata
     7021 gcctgcgtcg cttcaatatt ggtattgaaa atggtgtcgt ccagattgcg atgaccgaca
     7081 aacgctccgg tattgatgtc cacggtggtc atcgcttcgg tctggtcgat aatcagataa
     7141 ccaccggatt tcagttctac tttgcgttcc agcgctcgct gaatttcgtt ttcgacatca
     7201 aagagatcga aaatcggctg gcgtccggtg taatgctcca gcttgctggt catctcggga
     7261 atgtactccg aggtaaactc cagcaatgct tcgtaagtca ggcgtgagtc aacgcgaatg
     7321 cggtcgagtt cggcgtcggc gaaatcacgc agaacacgct gcgccagcgc cagttctccg
     7381 tacagctgat aacgggtctg cgggcgtttt ttacgctcca taactttggt ccagacgcgt
     7441 ttcagataag cggcatcgga ggccagttca gcctcgccaa ccccttccgc tgcagtacgg
     7501 atgataaacc cgccctgctc gtcgcaatac tctgcgacca cttttttcag gcgttcacgt
     7561 tctgattcgc tttcaattcg ttgggatacc ccaacgtgag aagcccctgg cataaacacc
     7621 agatagcgag aaggcagcgt gatatcggtg gtcaggcgcg cacctttagt gccaagcgga
     7681 tctttcacca cctgcaccat cagatcttgc ccctgacgga ccagttccga gatgtcgcgc
     7741 accgtgaatt gcttttgttc ttcacccgcc acacattcag tgtgcggcat gatgtcagat
     7801 gcatgaagaa acgcggcttt atccagccca atatctacaa aagccgcctg catacccgga
     7861 agtacacgac ttacacgacc cttgtagata ttgcctacta ttccgcgtcg cgcctcacgt
     7921 tcaatatgaa tttcctgcag aataccgcca tcaatatacg ccactcgagt ttccgaaggc
     7981 gttacgttta ccaacaattc agccgtcatg tttatccctt ttctcacgca gtgcgttaaa
     8041 attacttaat aattcatacg tttcaaccag cggtaagccg actacggcgt gaaagctgcc
     8101 atttatcttc ctgacaaaac agccacccag cccctgaata ccgtatgcac ctgctttatc
     8161 taacggttcg ccgctggcga catagcccgc gatgtcttcg tctgttaacg ttctgaaagt
     8221 cacatcggtg accaccaggc aatcgagaat gtgctggctg tcagccaacg ccactgccgt
     8281 catcacctga tgggtctgac ccgataattt gcgcaacatc tgcgccgcat gctctgcgtc
     8341 gcgcggtttc tcgagcactt ctccgttcag gataacgata gtatccgcac ccagcaccgg
     8401 gagatcctgc gccgtttgcg cgacacccgc ctgtgctttc tcgcgcgcca gacgcacaac
     8461 atactgctgc gcgctctcct gcggctgacg ctgctcctca atgcccgtaa caatacgttc
     8521 aaaggtcacg ccaagttgcg caagtaactc ctgacgacgc ggagaaccgg aagctaaata
     8581 cagagaagtc atagaaacct ttattgcact gcaaactgct gacggacttt acgcatcagc
     8641 aagaaaatcc acggccagag caccccattg actacactac tccagaacac ttccggtctg
     8701 aaagagacgt taatcactaa aaactctgcc cagaaaacaa taatatccac caccagcgaa
     8761 agcaacatga cgaccagcgc ctgctgccat aatgcgaggt tgcggaaaag ctggtatttc
     8821 agcgccacca ggtaagcaat gatgctcatc gccaatacgc gtacgccaag cgtcgagccg
     8881 ctgatcagat ccagtatggc acccatcaca aaacctgtgc ccacatttac gcgatggggc
     8941 aaggccagga tccaatacaa caagatgagt aacacccagt ttggccggaa aacaatcagg
     9001 ttatccggcc agggcatgat ctgcagcaac agcgcaatga ggaaagagag ccagattacc
     9061 cagcgtccct ggctacgata gctcgccact attgtcctcc cggcgcacgc gcaggcggtt
     9121 gagcaccact ttgcggcggc gtagcccttt gtggagagcg attagcagcg ggctgtgtgg
     9181 gcgcagcagg cgcagtaact gcatttcctg tcgccggttg ctgcggagtc ggctgagtga
     9241 tccccgttgc cggttcaggt aactttggcc ccatcgcgtc tggcgaaggc aatacctgcg
     9301 gcatcatctg catcaaacgt tcattagcaa cacgatgcac ctcttccggc gtcatcgggt
     9361 tagcgccgtt acgatccgcg ccccacagca gcagcagata acgcaaacgt tgcagtcctg
     9421 cagtcggacg cgcctgaatc acagtataag cgcgctgggt atcgagtttt acggaagaga
     9481 caaccgcgac cggatagcct tccgggaaac gaccgcccag accggaagtc accagcacat
     9541 caccaacacg aatgtccgta ttcgctggca gatgctcaag ctgcaaatca tccgtacaac
     9601 cgttaccggc tgcaattacg cggatatcgt tgcgcagcac ctggattggc agcgcgtggg
     9661 tcgcatcaca aatcagcagc acgcggctgg tcagtttagc gacggccacc acctgaccaa
     9721 caacaccttt gtcgctgata accggctggc cttcataaac gccattaacg ctacctttat
     9781 cgataacaac ttgatcgcta taaggatcgt taaccgtgga gataacctga gtcaccattt
     9841 tctgctcatc ctgacgcagc ggggaaccca gtagctcgcg cagacgcgcg ttctcctgtt
     9901 tgtattgtcc aagcatcagc agttcactgt ttttcagcaa cagttcctga cgtaacgccc
     9961 ggttttcaag ttctagttgg tcacgcgagg ccagcgtctg cgatacgcca tccagcaatt
    10021 cacgaggagc attggaaaca aagtagaaag gactgacggc ggtatccata taagtacgga
    10081 tttgactgaa cgtccccagg cggctgtcgg caataataat gccgagcgcc accagcaccg
    10141 ccagaataag gcgaatctgt agcgacgggc cacggctaaa aattggcttc ataagttatg
    10201 cgtattctcg tatcagacca ggcagggtaa acagacactt cccctgcctg catccgatta
    10261 ctcttcgctg aacaggtcgc cgccgtgcat gtcgatcatt tccagcgctt tgccgccacc
    10321 gcgcgccaca caggtcagcg ggtcttcagc aacaacgact ggaatgccgg tttcttccat
    10381 taacaaacgg tcaaggttac gcagcagtgc gccaccaccg gtaagcacca tgccacgctc
    10441 ggagatgtcg gaagccagtt ccggcgggca ctgttccagt gcaaccatta ccgcgctcac
    10501 aataccggtc agcggttcct gcagtgcttc gaggatttcg ttggagttca gggtaaagcc
    10561 gcgtggaaca ccttctgcaa ggttacggcc acgaacttcg atttcacgta cttcatcgcc
    10621 cgggtaagcc gaaccgattt cgtgcttgat acgttctgcg gtggcttcac cgatcagaga
    10681 accgtaatta cgacgtacat agttgatgat agcttcgtcg aaacggtcac caccaatgcg
    10741 cacggaagag gagtaaacca cgccgttcaa ggagataaca gcaacttcag tagtaccacc
    10801 accgatatca accaccatag aaccggtcgc ttcagaaacc ggcaggccag caccaattgc
    10861 ggcagccatc ggttcttcaa tcaggaagac ttcacgggca ccagcgccct gcgcggattc
    10921 acgaattgcg cggcgttcaa cctgggtcgc gccaaccggc acacaaacca gaacgcgcgg
    10981 gcttggacgc ataaagctgt tgctgtgcac ttgtttgatg aagtgctgga gcattttttc
    11041 agtcacgaag aagtcggcga taacgccgtc tttcattggg cgaatggcag caatattgcc
    11101 cggcgtacgg cccagcatct gcttcgcgtc atgacctact gcagctacgc ttttcggtga
    11161 accggcacga tcctgacgaa tggccaccac ggaaggctca ttcaatacga tgccttgtcc
    11221 ttttacataa atgagggtat tcgcagtacc caggtcaatg gacaagtcat tggaaaacat
    11281 gccacgaaat tttttcaaca tactaaggga taatcctgaa agctggggcg gaaaagaaaa
    11341 tccgcttact ttaccaacca cacgcagcag cgacaaggcg caaaaatcat ctgctacggt
    11401 gaaaattagt gcagttcgtt tcctttgtta caaatctctg cctgagtcca gaaaggctta
    11461 atgcatcagc agcattcctc gcctgtttgc aaccgcgtaa ggtcattcat ctgcatatgt
    11521 gctgcaacaa tctggcgagc agacaagcac actcccatga gacgcagcgc gcgttattct
    11581 acgtgaaaac ggattaaacg gcaggttaaa ccgagtatct ttgtgaatat tttttcacgt
    11641 tagtatcaag tggctgtgag gacgcgaaaa aatccccttg cccacctgta acaccgcgct
    11701 gaatcagggt ctgccactcg cttcgcgaac gcacgccggt ggcgtaaacc tgggtgctgg
    11761 tcccggagca ggcttccacc aggctttgaa ccagcagttg gttctccgtt cgcttctcaa
    11821 tgtttctgac cagccccgga tggagcttga gtaactcaac attaagttct ttgatccaac
    11881 tggtgcttac cagcgtcaaa ccagcctggt tgacggctac ccgtacccct aaagcattca
    11941 ctaaacgaat aacaggttgt aagcgactga tatgttgacc aacatcggcc tctgcaagtt
    12001 caataattat gtgtcttcgt tgcgattttt cacattgcat taacgtatcg cgcagccaac
    12061 gctgaaaacg cgggcgaatc agcgactcaa cggtaacctg aatcgccaga ttttcctctg
    12121 gccagtaacg caatagtgga ataagacggc tgatttgcag gcggtcatac tcttccgata
    12181 aaccaaactg caagaccatc ggcatatact ctgccgagct aacctcttca ttaccatcga
    12241 aaatgcggca catgagttca cgatgatgaa cccggccttc gcgagtaacc gccggttttt
    12301 gataaaggcg cgggccgccg cgactgagca tttgctcgat aagcgtacgc cagcgaacat
    12361 taccgcgtcc tttttcaggc aacgagtcat cgtaaatagc ccagctattg ccgccctgca
    12421 atcccgcatt acgcgtggca gactctgcat gttccattac ctgctcggta tcctgaccac
    12481 tacgccaggc acagatacca atgtggatca tatcgtcgcg atcgagcatt ttattgttcg
    12541 gcaaggtatc gacggctttg attaactgac tggcgatgct ctctgcctct tttaacgtcc
    12601 ggtgcggtaa cagcgcagca aaatcactgc ggtggtaacg cgccagcagt gcgccagggt
    12661 agcgcatcat aaatgtcgat agcagattcg tcagagagaa gaactgttct tcaacctggc
    12721 tgtgccccca ggtatcactc aacatattga aatccggcag acgaatcatc atcacgatcc
    12781 cgtgggtacc tactttctct tgatcttcca gtaacgttgc taactgatta tcgaaaaaga
    12841 gtcggttatt gaggccggtt ttcatgtcct gggcggcata agagcggatc agcgtatcaa
    12901 gacggctgtg ttgttcgcgt gcgttctgaa tttcacaaag cagcgtatcc agcgcactgc
    12961 tggttctggg cggccattca tagatggttc ccaacacatt agagccacgc tcaccgttta
    13021 agatacgagt agcccgggtt tccagcaatt cttgcccggc aagttgccgt tgtaaccagc
    13081 gtaccgccag gaagagcata agaatgataa agccaatcgc ccccgtgagc ggcgcggtag
    13141 tcattaaaga atggaaatag ttgcccatcg gatcctgata aaccagacgc agcgacatcc
    13201 ccggatgctt tatcaacgga acgctcagtt cacgaaacag atcgttggtg ccaaccggac
    13261 gataactacc atttctggcc agggtataaa cctgcttatc accatggagc aggtctacac
    13321 gaacgatatc tgccgacatc attaattcgg taatttgtgg ccttaatgtg ctgaagtcat
    13381 tcgacactag gtgggtatca atcgccgtcg ccaccgcctg aacacgatga ctaaacttat
    13441 actgaatggc gttgtagaaa cttagcgaac agcccagcaa agtcacaaaa attgttaacc
    13501 cggtgagcag cgtgacaaag gccgaaaatt tcgtcgttaa tctcatcctt gtgttaactc
    13561 cgatagtgag gaagcgggca tactagcaaa tcagatttat ctcgcaattt attgcgcttc
    13621 atcggctttg cttttccatt agcgagtata gtcttcagaa attattttcc aatccatcat
    13681 gcacatgagg accacttatg caggcgttac ttttagaaca gcaggacggc aaaactctcg
    13741 catcagtaca gactctggac gaaagtcgcc tgccggaagg cgatgtcacg gttgatgttc
    13801 actggtcgag cctgaactac aaagatgcgc tggcgattac cggtaaggga aaaatcatcc
    13861 gaaattttcc gatgattcct gggattgatt ttgccggaac tgtacgcacc agcgaagatc
    13921 cgcgttttca tgccggtcag gaggtgttac tcactggctg gggcgttggt gaaaaccact
    13981 ggggtgggct ggcggaacag gcgcgagtga aaggtgactg gctggttgcc atgccgcaag
    14041 ggctggacgc gcgtaaagca atgattatcg gtactgccgg ttttaccgcc atgctgtgtg
    14101 tgatggcgct ggaagatgcc ggtgttcgcc cgcaggatgg ggaggttgtc gtgacgggtg
    14161 ccagtggtgg cgtcggcagt accgccgtgg cgctgctgca taagttgggt tatcaggtcg
    14221 ttgctgtttc cggtcgcgaa agtacccatg aatatctgaa aagtttaggt gccagccgta
    14281 ttcttcctcg cgatgagttt gccgaatccc gtcctctgga aaaacaagtc tgggctgggg
    14341 caattgacac cgttggcgac aaagtgctgg caaaagtgct ggcgcaaatg aattacggcg
    14401 gctgcgtggc ggcctgtggt ctggcgggtg gttttactct gccaaccacg gtcatgccat
    14461 ttattctgcg taatgtccgt ttgcaagggg tggattcagt aatgacgcca ccagcacgcc
    14521 gcgcacaagc ctggcagcga ctggtcgccg atttaccgga atcattctat acccaggcgg
    14581 caaaagagat atctctggca gaggcaccga agtttgccga ggccatcatt aataaccaga
    14641 tccagggtcg cacgctggtg aaggttaact aaccatttag cagggaataa taagagaggg
    14701 aactcatttt gaaatcatta ttcaatcggt taacgggaaa agcggttagc cggacagctt
    14761 tcgtcgaaca tcttggtcat gaagttgtac aacatcatcc aaactggaaa gtcatgattt
    14821 cgactgacca taaattgatg cgcattgata ctccaccaaa cagccattat tgatacgcct
    14881 ccgtcgcctg ttaggtttat ggtgctttgc cttggcgacg ctacgcttaa tctgttactg
    14941 atttctggta ccatgggggg aataatctga ttttatttga ttacaaatta accacttaaa
    15001 cctattgaat gcggaacatt gtcagtcggt taattttgcg tgctttagca ttcacatcta
    15061 tccagacgat gaagtgaaaa ttgcgtaatc ctcagcaacc gctgcgtaat gtcgtctatc
    15121 ttgtcgcgat cctggcatcc ctacattatt tgtggtctgt gaagattatc tcattgcagc
    15181 ccctcatctt cgcagggctg gctttacagc ttttcacctt acgttataag aagttccgtc
    15241 gatgatggcg ctaatttcgt gaattgtgcg gcttgttgca aattacacgg tgttgaaggt
    15301 tatttacatg ttagctgttg attatcttcc ctgataagac cagtatttag ctgccaattg
    15361 ctacgaaatc gttataatgt gcgacctcgt cctccctgac gcagtttttg cgctgcggaa
    15421 aaggtgacat tggcgcaacg aaggtatatt ttgttttttg ccggaggata gcagcagatc
    15481 gctgcacaat gtccgtcaag tctaacattg acactctggg gcaaaataga ccggcgtccc
    15541 ggcctgctgg aatttatcgc tatgcataca gctgtcgggg catacgcttt acagacggcg
    15601 gtgaaacgcc tgtcacaatc acactaaaca aagagtacgg aacccactca tggatattcg
    15661 taagattaaa aaactgatcg agctggttga agaatcaggc atctccgaac tggaaatttc
    15721 tgaaggcgaa gagtcagtac gcattagccg tgcagctcct gccgcaagtt tccctgtgat
    15781 gcaacaagct tacgctgcac caatgatgca gcagccagct caatctaacg cagccgctcc
    15841 ggcgaccgtt ccttccatgg aagcgccagc agcagcggaa atcagtggtc acatcgtacg
    15901 ttccccgatg gttggtactt tctaccgcac cccaagcccg gacgcaaaag cgttcatcga
    15961 agtgggtcag aaagtcaacg tgggcgatac cctgtgcatc gttgaagcca tgaaaatgat
    16021 gaaccagatc gaagcggaca aatccggtac cgtgaaagca attctggtcg aaagtggaca
    16081 accggtagaa tttgacgagc cgctggtcgt catcgagtaa cgaggcgaac atgctggata
    16141 aaattgttat tgccaaccgc ggcgagattg cattgcgtat tcttcgtgcc tgtaaagaac
    16201 tgggcatcaa gactgtcgct gtgcactcca gtgcggatcg cgatctaaaa cacgtattac
    16261 tggcagatga aacggtctgt atcggccctg ctccgtcagt aaaaagttat ctgaacatcc
    16321 cggcaatcat cagcgccgct gaaatcaccg gcgcagtagc aatccatccg ggttacggct
    16381 tcctctccga gaacgccaat tttgccgagc aggttgaacg ctccggcttt atcttcatcg
    16441 gcccgaaagc agaaaccatt cgcctgatgg gcgacaaagt atccgcaatc gcggcgatga
    16501 aaaaagcagg cgttccttgc gtaccgggtt ctgacggccc gctgggcgac gatatggata
    16561 aaaaccgcgc cattgctaaa cgcattggtt atccggtgat tatcaaagcc tccggcggcg
    16621 gcggtggtcg cggtatgcgc gtagtgcgca gcgacgctga actggcacaa tccatctcca
    16681 tgacccgtgc ggaagcgaaa gcagctttca gcaacgatat ggtttacatg gagaaatacc
    16741 tggaaaatcc tcgccacgtc gagattcagg tactggctga cggtcagggc aacgctatct
    16801 atctggcgga acgtgactgc tccatgcagc gccgccacca gaaagtggtc gaagaagcac
    16861 cggcaccggg cattaccccg gaactgcgtc gctacatcgg cgaacgttgc gctaaagcgt
    16921 gtgttgatat cggctatcgc ggtgcaggta ctttcgagtt cctgttcgaa aacggcgagt
    16981 tctatttcat cgaaatgaac acccgtattc aggtagaaca cccggttact gaaatgatca
    17041 ccggcgttga cctgatcaaa gaacagctgc gtatcgctgc cggtcaaccg ctgtcgatca
    17101 agcaagaaga agttcacgtt cgcggccatg cggtggaatg ccgtatcaac gccgaagatc
    17161 cgaacacctt cctgccaagt ccgggcaaaa tcacccgttt ccacgcacct ggtggttttg
    17221 gcgtacgttg ggagtctcat atctacgcgg gctacaccgt accgccgtac tatgactcaa
    17281 tgatcggtaa gctgatttgc tacggtgaaa accgtgacgt ggcgattgct cgcatgaaga
    17341 atgcgctgca ggagctgatc atcgacggta tcaaaaccaa cgttgatctg cagatccgca
    17401 tcatgaatga cgaaaacttc cagcatggtg gcactaacat ccactatctg gagaaaaaac
    17461 tcggtcttca ggaaaaataa tttgtgacgc gcggtgtttt catcgcgctt agcaaagaac
    17521 gtaatcaacg ccgggcctgt taatgctacc cggcgttttt attgccgtta actaacggta
    17581 gcctgcaatt tccgctttca acgcgctaat ttcagcatcg ccatcaacaa ttcccagctc
    17641 aaagcagtat ttctttgttt cccctggttc aataaagggc agacgcccct gcgctcgttc
    17701 tttatcacga ccgtcaagaa aactattact gacctccagt cccataacat attcacccgc
    17761 gcctaaattt ttccactgga taaagttggg tagctgcatg atgtcgaatt tctcgtaaat
    17821 tccccacccc agttcggcgt tcaccagcgc aactttactc atcccctgac tgtcgctatt
    17881 caacgtgaga tagtaaacct gctcgtcaaa accgggctgt ggggtacaaa tctccagatg
    17941 actggctaac ccttccgccg catgcggggt tcgtggcgtt acgcttttag ccgacagcaa
    18001 aatttctgcc tgtggcgata ataaaggcca gccgtaatta aagtgataaa gaatttgcag
    18061 cggctggcgg gtgaaaccat gattagtcac cttgtcttca atgcgcatta cattttcgcc
    18121 ataacggcag cgaatggtcc tttccagcgt cagattttca ccgaacaatc gtgtttcccg
    18181 tactttgcca ttgatgacaa attcaatgct gtcgtcggtc cgttctgtgc gataccccac
    18241 ctcttctgct ggagtcgcag ccaaacgccc gtgtaacccc agcgtttctc cgtcatcctc
    18301 acagggcgtg ccaatgtagc taagtccgca ggtagtcagt aaccccgcgt agaagctacg
    18361 taaaaacccg ttgccctgag actcaaaaaa agcgggggcg acaatgccgt tgggggtgat
    18421 aaaagagagc gcagtgtcct tgtagctggc ccatgcaata tccagcgcgc gatcttttac
    18481 caccgtaaat gccagtcccg caccagtacg gacatcaaac gcttctgtcc cctttgctct
    18541 gccactacgt aactcatagt gtttaattcc ggcaacctgc gagagatcgc caatccacga
    18601 ccaaatttcg tttttcattg tgctgcttcc cgaatggaca tgtcatggcg ctggatgaac
    18661 gcttcgattt ccgcaaggcg agacgttccc tccattgggc cacgtcgggt taccgccagt
    18721 gcaccgcagg cgttggcata ttgtaatgcc cgatgggcgt caaaccctag ctggcggcag
    18781 gcaatccatg cgccaccaaa acagtctccc gcaccggtcg ggtcaacttc ttccaccgga
    18841 tagctctcaa catgaaactg ctcattcgcg gagtagtaac tggctccttg attgccgcgc
    18901 ttcacaatca cctctttaac accttcttca agaaaaccag cgattgcacg ctccggcgtc
    18961 gagtgtggcg acaagagcaa cacttccccc tcactgggca tatagatatc cgttaactca
    19021 agaacaaagt ggagggcatc gcgcatttct ggaatatcaa gcatttcttt acgaatattc
    19081 gggtcgaaag aaataacgcc accatttgct ttaacgatgg ttactgcttt ttttactgcg
    19141 tctaccatat ggaatgaaaa cagggatgaa cccataatat gaaaatgggt acaatccttc
    19201 agaatatttt catcaacatg ctgtgccgat aatttaccgc aagcggcatt cttgatatta
    19261 aaaataaaat cgcgatcgcc agagttatga taagtaacaa aggcactacc ggtggcttcc
    19321 agaggtaaga ctgaaatccc cctgatgtcc acaccgtcag cagccagacg atgtatatta
    19381 atatcaccaa agccatcatt ccccacgcag ctaatgatgc cgcacgggac gccaagacgt
    19441 gtcacctggt cgataaaaat agcgggcgca ccactgggat aagggccgtt ccagatacct
    19501 ggttgatcaa atggctggcc gattttactc gccataattt caaccagaat ttctccaatt
    19561 gtgaagacct tactcatatg attaattcct tgaaatgatt agctgcgttt tcttttattt
    19621 cttacgtcaa aataaacggc tacaagaatg actaatcctt taaccaccat ctggtaaaaa
    19681 taaggaacat tcattaagtt catgccatta ttaataacgc cgatggttaa tgcgccgatc
    19741 accgtcccca ccacggttcc atagccgccg ttcaggctgg tgcctcccag tacagccgcg
    19801 gcaatagcat ccagttcgta gctcaacgcg gaattcggtt gcccggaata gaggcgtgag
    19861 gcaaggatca tcccgctgat acctgccagc acggctgtaa tcataaagac tttaattttc
    19921 aagcgggtga catttatccc tgaataaatt gccgcttctt tattaccacc agtaatataa
    19981 atctttcgtc caaaggttgt tttagtaaga atgaaatggt taatcagcaa taagataatt
    20041 aatatccaga ttggaatagg tatatagaga aattctccat tccccagcgc cagaaaggca
    20101 tcatctttaa tcatgatggg cataccatcg gtcactatat aagcgatacc acggaagatc
    20161 cccatagtag caacggtgac aataaacgac gggatccccg ctatagccgt caagccacca
    20221 tttatcatcc ccagagccag tgctgcaata atggataatg ggaccaataa aacaaatgga
    20281 attccccatg aagccccaag cgcagcaaat gtgccaatca atgcaatggt tgaaccgacc
    20341 gataaatcaa tatcgccaag caaaagcaca taagtcattc cataggcggt aattgcaatt
    20401 atcgaaacct gaagaataat attagtaatg ttattggcgg taaaaaaact gtcattaata
    20461 aaactgaaca gaatgattaa caccaccatt ccactgagga taccaccata ttgcctgaag
    20521 acttttttaa aattatttgc aatgggaatt tccgaaggtt tcggactggc aagagttttc
    20581 tctgtcattt aacgtactcc tgttgcagcg gtcattacct gtgtctgcgt taaattttca
    20641 catgaataaa tatcggcaat cgttttatta cgcattacca ccagacgatc gctaagtgcc
    20701 atgacttctg gcagttcaga ggacaccaga ataattgctg tccctgccgc agccagttga
    20761 cgtatcacgt tatagatttc aaattttgcg ccgacatcca ctccgcgcgt tggctcatcg
    20821 agcaataaaa tacgcggagt tttctccagc cacttcgata acacgatttt ctgttgatta
    20881 ccgccgctca gtgctcccac tgcctgatgc acagaactca ctttagtgtt cattctggca
    20941 attgattgca gcgtcttcgc ctcttcttgt cggtgattga tgagtcccat ccgcgaagcg
    21001 ttatattgaa acgccacatg gtgcgtgttc atattgacgt cgtgcatcag cactaaccct
    21061 tcttccttgc ggttttcggt cacaaaagca atgccatgat caatagcctg tttgggatta
    21121 gcgatcctga cttccttacc atcaatcaac acagtcccgt gatacgaaac gaggccaaat
    21181 agcgccttca tcacatcgga acgcccggcc cccaccaggc caaaaaatcc gaggacttcc
    21241 cccggacgca cagcgaaact gacctcttta aaaagcgaag gatgggaaag attttttact
    21301 tccagttttg caggaacatt ttcatccggt ttttccccaa gacgtgctgg ccaaatattt
    21361 ttcatttccc gaccaaccat ttgggcaatc aagtgctgga tatcactgtt tttcttttca
    21421 tgcgtagcga tatactcacc gtcgcggatc accgaaatat catctgaaat ccgcataatt
    21481 tcatccatgc gatgagagat ataaataacc gcacagcctc gctgctttaa tttttcaata
    21541 atgttaaaca gaatctccgc ttcattatca cttagcgagg atgtcggttc atccataatc
    21601 acgacatccg ctgcatagct taaggctttg gcaatttcta ccagttgcct atgtgccatc
    21661 gacatattgc cgaccatttc cagagggtct agtgggatgc ccagttccgc gaggaggttc
    21721 tggcaatctg cgagcatttt tttatcgtta atgatgccaa aacgtcgcgg ttcattattg
    21781 gcataaatat tttccgccac ggtcatatta ttactcaggc tcagttcctg aaaaataatc
    21841 gcaatcccta actggcgaga atgctgcgga ttattaatgg tgatggtttc accacgtagt
    21901 gttatcgttc cctgggttgg cggataaaca ccggccagta ttttcattaa tgtcgattta
    21961 ccggcaccat tctcgcctaa taaagtatgc acccgccctt tacgtaaaga gagtgtaata
    22021 tcttttaatg cctgaacatg accaaatgtc ttcgcaatat gcgaaacatc aagaataaca
    22081 tccgtcataa tgacctcggt gtcacaacaa aacgccaata acgttttgtt gtgaggtgaa
    22141 gatatttact atttacctgt ataaacgccg ggttgtaccg ggatcatttt ttctaccggt
    22201 ttaccattga ggtgatcaac agcggcatca atggctttag cacccatttg atcgggatat
    22261 tgccgaatca cggccgcaaa cgttttttca ctgtcaacgg catcacgcgc ttctttcatg
    22321 ccgtcaaaac caatgatttt gatgttgtcg ttatgcgcag attttgctgc gataaccgct
    22381 gccagcgccg catcatcacc aaagccaaac agaccattca ggttggcatt tgcctgcata
    22441 atgttttgcg cagtcgttaa cgcttcagcc cgcgtaatac caggctgttc agcaacaatt
    22501 ttaatatccg gtgtatcact taaccctttc ttaaaaccat caacgcgcgc aaccaccgat
    22561 tgtagtgccg gataagaaat aatggctaca gtacctttac catgcaaaag tttacccatt
    22621 aacttgcctg cttcaacgcc tcccgcatag ttatcggtcg caacatgcga tacgaccgga
    22681 acgccttccg cagcgacgtc aacggtgata acgggaatac cggccttatc ggctttaatt
    22741 atcgctgcct ggacgccttt cgaatcgacc ggagagataa taatggcatc cactttttta
    22801 gtaataaaat cctcaacatc agaaagttgt ttgtttaaat cctgattggc aatagagaca
    22861 cttaaattga cattgtcttt ttttgcctct tctttcatgg cattcgcaag atcaatatag
    22921 aaaggatgtt gttgtgttaa taatgacgca ccaattttat actcttttgc caacgaactt
    22981 tgcgagaaca atgccaggct caccaacata gctgaaccaa gtaatttaat atttttcata
    23041 aacaatcctc attatttttg tagggtacaa cgtcttaaat ttttccttca ctaccacaga
    23101 cgccaataat acgaattacg acttctttca tggcggcctt tgcgggctgc atatagtgac
    23161 gtggatcatt tgccgcagga ttgttgtaga aatatccttt taacgcatcg gcaaacgcaa
    23221 ttttcaattc tgtcgcgaca ttcactttac aaataccgcg acgaatacag tgctgaacgt
    23281 cctgttccga taagcccgaa gcaccatgca acaccagcgg aatctccact cgctgacgaa
    23341 tctgttccag ccgcacaaaa tccagtcgtg gttcatgcgc atataaacca tgtgctgtgc
    23401 cgattgctac cgccagggag tcaatttggg tacgttgaac aaactctgca gctgcggctg
    23461 gtgaggtata agcggaatct ttttcatcca cccgcagatc gtcctcctgg ccgccaagac
    23521 gccctagctc ggcctcaacg ctaacatcaa agcgatggca aaaggtggta acttcttgca
    23581 ctcgcgcaat gttgtcttca aatggcaaat gggagccatc aatcatgaca gaacggatcc
    23641 ctgcgcgaac cttgttaaaa atgtcatcac tctcttcatg atggtcgaga tgcaggacta
    23701 acggcaggtt gtactcgcga gccagcgatt gtactaacga aattaaactt cccacgccac
    23761 cataacgata tgtaccgggt gttcccgcca gcataacggg agagcgaagt tgtgcagcgc
    23821 tctcaacaac tacctgtaaa gtttcaaggt tatgaatgtt gaaagcaggg acggcatatc
    23881 cctctgactg ggcttttttc agcatgtttt tgcttgaaat aatatacatc gcagctcctg
    23941 ttatttgtcg ttcgccttac aaaatgaaga aagagaatct tcactttcat tttgaaagca
    24001 aactagcaca gcaaaagcag caaaaagcgt gatgcatgtc gaacatcatc aaaataactt
    24061 acaaaaaata acttaattta
//
LOCUS       DADTFG010000072        23887 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.072, whole genome shotgun sequence.
ACCESSION   DADTFG010000072 DADTFG010000000
VERSION     DADTFG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.072"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..2973
                     /locus_tag="J1138_002612"
     CDS             <1..2973
                     /locus_tag="J1138_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA8454388.1"
                     /translation="TNTADIATNASSITTLNADVDTLEKDALLWNGTAFSAKHGVDGA
                     DSKITNVAAGSLADDSTDAVNGAQLKATNDSIAIMGDTISDIEDDALLWDGSSFSAKH
                     GTKGDNSKITNLLAGTVSSDSTDAINGSQLYGVADSFTSYLGGGADISDTGVLSGPTY
                     TIGGTDYTNVGDALAAINTSFSTSLGDALLWDATAGKFSAKHGINNAPSVITDVANGA
                     VSSTSSDAINGSQLYGVSDYIADALGGNAVVNTDGSITTPTYAIAGGSYNNVGDALEA
                     IDTTLDDALLWDTTANGGNGAFSTAHGKDKTASVITNVANGAVSATSRDAINGSQLYS
                     TNKYIADVLGGDAEVNADGSITAPTYTIANTDYNNVGEALDALDNNALLWDEDAGAYN
                     ASHDGNASKITNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNTSKNYIE
                     DNGAGINYVRTNDSGLAFNDASAAGIGSTAVGYNSVADGDNSVAIGHGSNSIVETSIA
                     LGSESVSSRVIVKGSRNTSVSEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGS
                     EAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIG
                     IGLNTLVLADAINGIAIGSNARANHADSIAMGNGSQTTRGAQTNYTAYNMDAPQNSVG
                     EFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTR
                     VTNIENGIGDIVTTGSTKYFKTNTDGADANAQGKDSVAIGSGSIAAADNSVALGTGSV
                     ADEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSN
                     ITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTES
                     KLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQG
                     STNSQGEYSAALGAGIQW"
     gene            3383..5038
                     /gene="lldP"
                     /locus_tag="J1138_002613"
     CDS             3383..5038
                     /gene="lldP"
                     /locus_tag="J1138_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBA8454389.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            5038..5814
                     /gene="lldR"
                     /locus_tag="J1138_002614"
     CDS             5038..5814
                     /gene="lldR"
                     /locus_tag="J1138_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBA8454390.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5811..7001
                     /gene="lldD"
                     /locus_tag="J1138_002615"
     CDS             5811..7001
                     /gene="lldD"
                     /locus_tag="J1138_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBA8454391.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLANNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQVLGKELPAALAPMAK
                     GNAA"
     gene            7049..7522
                     /gene="trmL"
                     /locus_tag="J1138_002616"
     CDS             7049..7522
                     /gene="trmL"
                     /locus_tag="J1138_002616"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBA8454392.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7577..8398)
                     /gene="cysE"
                     /locus_tag="J1138_002617"
     CDS             complement(7577..8398)
                     /gene="cysE"
                     /locus_tag="J1138_002617"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBA8454393.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8478..9497)
                     /gene="gpsA"
                     /locus_tag="J1138_002618"
     CDS             complement(8478..9497)
                     /gene="gpsA"
                     /locus_tag="J1138_002618"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBA8454394.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9497..9964)
                     /gene="secB"
                     /locus_tag="J1138_002619"
     CDS             complement(9497..9964)
                     /gene="secB"
                     /locus_tag="J1138_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBA8454395.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10028..10279)
                     /gene="grxC"
                     /locus_tag="J1138_002620"
     CDS             complement(10028..10279)
                     /gene="grxC"
                     /locus_tag="J1138_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBA8454396.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10420..10851)
                     /locus_tag="J1138_002621"
     CDS             complement(10420..10851)
                     /locus_tag="J1138_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBA8454397.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11096..12640
                     /gene="gpmM"
                     /locus_tag="J1138_002622"
                     /gene_synonym="pgmI"
     CDS             11096..12640
                     /gene="gpmM"
                     /locus_tag="J1138_002622"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBA8454398.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12650..13933
                     /gene="envC"
                     /locus_tag="J1138_002623"
     CDS             12650..13933
                     /gene="envC"
                     /locus_tag="J1138_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBA8454399.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13937..14896
                     /locus_tag="J1138_002624"
     CDS             13937..14896
                     /locus_tag="J1138_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBA8454400.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(14883..15917)
                     /gene="waaH"
                     /locus_tag="J1138_002625"
     CDS             complement(14883..15917)
                     /gene="waaH"
                     /locus_tag="J1138_002625"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBA8454401.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFLTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIRFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16156..17181)
                     /gene="tdh"
                     /locus_tag="J1138_002626"
     CDS             complement(16156..17181)
                     /gene="tdh"
                     /locus_tag="J1138_002626"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBA8454402.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMTELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17191..18387)
                     /gene="kbl"
                     /locus_tag="J1138_002627"
     CDS             complement(17191..18387)
                     /gene="kbl"
                     /locus_tag="J1138_002627"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBA8454403.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(18662..19519)
                     /gene="yibB"
                     /locus_tag="J1138_002628"
     CDS             complement(18662..19519)
                     /gene="yibB"
                     /locus_tag="J1138_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBA8454404.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKSRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLAVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            19823..20755
                     /gene="rfaD"
                     /locus_tag="J1138_002629"
     CDS             19823..20755
                     /gene="rfaD"
                     /locus_tag="J1138_002629"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBA8454405.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            20765..21811
                     /gene="rfaF"
                     /locus_tag="J1138_002630"
     CDS             20765..21811
                     /gene="rfaF"
                     /locus_tag="J1138_002630"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBA8454406.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGVMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNQILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            21815..22807
                     /gene="rfaC"
                     /locus_tag="J1138_002631"
     CDS             21815..22807
                     /gene="rfaC"
                     /locus_tag="J1138_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBA8454407.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIIINKLTSEIR"
     gene            22804..>23887
                     /gene="rfaL"
                     /locus_tag="J1138_002632"
     CDS             22804..>23887
                     /gene="rfaL"
                     /locus_tag="J1138_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBA8454408.1"
                     /translation="MTSTLFFSFEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLVQSPDAGAGMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDIEKIVFFSFISALGLRCFSELIAYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFAVLSAIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPQWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNVVQNKYRSPY"
BASE COUNT         5996 a         6108 c         6041 g         5742 t
ORIGIN      
        1 actaataccg ccgatatagc tacaaacgct agcagtatca ctactctgaa cgcggacgtt
       61 gataccctgg aaaaagatgc cctgctctgg aatggcacag cattcagcgc caaacacggt
      121 gttgatggcg ctgacagcaa aatcaccaac gtcgccgcag gtagcctggc tgatgacagt
      181 actgatgcgg taaacggtgc acagttgaaa gcgaccaacg atagcatcgc tattatgggc
      241 gataccatct ccgatatcga ggacgatgcc ctgttgtggg atggttcttc cttcagtgcc
      301 aaacacggta ctaagggcga taacagcaag atcaccaact tactggcagg cactgtatcc
      361 tctgacagca ctgatgccat taatggctca caactttatg gcgtagcgga ttcatttacg
      421 tcatatcttg gtggtggtgc tgatatcagc gatacgggtg tattaagtgg gccaacctac
      481 actattggtg gtactgacta cactaacgtc ggtgatgctc tggcagccat taacacatca
      541 tttagcacat cactcggcga cgccctactt tgggatgcaa ccgcaggcaa attcagcgcc
      601 aaacacggca ttaataatgc tcccagtgta atcactgatg ttgcaaacgg tgcagtctcg
      661 tccaccagca gcgacgccat taacggttca caactttatg gtgttagtga ctatattgcc
      721 gatgctctgg gcgggaatgc tgtggtgaac actgacggca gtatcactac accaacttat
      781 gccatcgctg gcggcagtta caacaacgtc ggtgacgcgc tggaagcgat cgataccacg
      841 ctggatgatg ctctgctgtg ggatacaaca gccaatggcg gtaacggtgc atttagcacc
      901 gctcacggaa aagataaaac tgccagtgta atcactaacg tcgctaacgg tgcagtctct
      961 gccaccagta gagacgccat taatggctcg cagctctata gcaccaataa gtacattgct
     1021 gatgtactgg gtggtgatgc agaagtcaac gctgacggtt ctatcactgc gccgacttac
     1081 accattgcaa ataccgatta caacaacgtc ggtgaagccc tggatgcgct cgataataac
     1141 gcgctgctgt gggatgaaga cgcaggtgcc tacaacgcca gccatgatgg caatgccagc
     1201 aaaattacca acgttgcggc tggtgatctc tccacaacca gtaccgatgc tgttaacggt
     1261 tcccagttaa acgcaaccaa tatcttggtt acgcaaaata gccaaatgat taaccagctt
     1321 gctggtaaca ccagtaaaaa ctacatcgaa gataacggtg cgggtattaa ctatgtacgt
     1381 accaacgaca gcggcttagc gttcaacgat gccagcgcag caggtattgg ctctaccgct
     1441 gtgggttata actctgttgc cgatggcgat aacagcgtgg caatcggtca tggcagcaac
     1501 agcatcgttg agacgagtat tgcgctgggt agcgaatccg tctccagccg tgtgattgtt
     1561 aaaggttctc gtaacaccag cgtatcggaa gaaggtgttg tgattggtta tgacaccacg
     1621 gatggcgaac tgcttggcgc gttgtcgatt ggtgatgacg gtaaatatcg tcaaatcatc
     1681 aacgtcgcgg atggttctga agcccatgat gcggtcactg ttcgccagtt gcaaaacgcc
     1741 attggtgcag tcgcaaccac accaaccaaa tactatcacg ccaactcaac ggctgaagac
     1801 tcactggcag tcggtgaaga ctcgctggca atgggcgcga aaaccatcgt taatggtaat
     1861 gcgggtattg gtatcggcct gaacacttta gttctggctg atgcgatcaa cggtattgct
     1921 atcggttcta acgcacgcgc aaatcatgcc gacagcattg caatgggtaa tggttctcag
     1981 actacccgtg gcgcgcagac caactacact gcctacaaca tggacgcacc gcagaactct
     2041 gtgggtgagt tctctgtcgg cagtgaagac ggtcaacgtc agatcaccaa cgtcgcagca
     2101 ggttcggcgg ataccgatgc ggttaacgtg ggtcagttga aagtaacgga cgcgcaggtt
     2161 tcccagaata cccagagcat tactaacctg aacactcagg tcactaatct ggatactcgc
     2221 gtgaccaata tcgaaaatgg cattggcgat attgtaacca ccggtagcac taagtacttc
     2281 aagaccaaca ccgatggcgc agacgccaac gcgcagggta aagacagtgt tgcgattggt
     2341 tctggttcca ttgcagccgc tgacaacagc gtcgcactgg gcacgggttc cgtagcagac
     2401 gaagaaaata cgatctctgt gggttcttct accaaccagc gccgtatcac caacgttgct
     2461 gccggtgtta atgccaccga tgcggttaac gtttcgcaac tgaagtcttc tgaagcaggc
     2521 ggcgttcgct acgacaccaa agctgatggc tctatcgact acagcaacat cactctcggt
     2581 ggtggtaacg gcggtacgac tcgcatcagc aacgtttctg ctggcgtgaa taacaacgac
     2641 gcggtgaact atgcgcagtt gaagcaaagt gtgcaggaaa cgaagcaata caccgatcag
     2701 cgcatggttg agatggataa caaactgtcc aaaactgaaa gcaagctgag tggtggtatc
     2761 gcttctgcaa tggcaatgac cggtctgccg caggcttaca cgccgggagc cagcatggcc
     2821 tctattggtg gcggtactta caacggtgaa tcggcagttg ctttaggtgt gtcgatggtg
     2881 agcgccaatg gtcgttgggt ctacaaatta caaggtagta ccaatagcca gggtgaatac
     2941 tccgccgcgc tcggtgccgg tattcagtgg taatgatcca ttaacaaatg ggtacgccac
     3001 attgcctgat gcgctacgct tatcaggcct acggggtgat tcagcactac cctgtaggcc
     3061 ggataaggcg ttcacgccgc atccggcaag ataaggcgca ccagatcaac aatctgaggg
     3121 caattctctg atgggaattg cccttttctt tattacctct ctcccagaat tggccctacc
     3181 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatgtt
     3241 gccaacacat aacatttagt taaccattca ttgtcattac ccctacacaa cacaattggc
     3301 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacggcgtg
     3361 tgtggactgc aggagacctg caatgaatct ctggcaacaa aactatgatc ccgccgggaa
     3421 tatctggctt tccagcctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     3481 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtggcaatcg cccttgccgt
     3541 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     3601 cttctacggg ttgtggccca tcgcgtggat cattattgca gcggtgttcg tctataagat
     3661 ctcggtgaaa accgggcagt ttgacatcat ccgctcgtct attctttcga taacccctga
     3721 ccagcgcctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     3781 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gctttaaacc
     3841 gctgtacgcc gccgggctgt gcctgattgt taacaccgcg ccagtggcat ttggtgcgat
     3901 gggtattccg attctggtcg ccgggcaggt aacaggcatc gacagctttg agattggtca
     3961 gatggtgggg cgtcagctgc cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     4021 tatggacggc tggcgcggta tcaaagaaac ctggcctgcg gtcgtggtgg cgggtggttc
     4081 gtttgctatc gcacagtacc tcagttccaa cttcattggg ccggaactgc cagacattat
     4141 ctcttcgctg gtatcgctgc tctgtctgac actgttcctc aaacgctggc aaccagttcg
     4201 cgtattccgc ttcggtgact taggggcgtc acaggttgat atgacgctgg ctcacacagg
     4261 ttacacggca ggtcaggtgc tgcgcgcctg gacaccgttc ctgttcctga ccgccaccgt
     4321 aacgctgtgg agtatcccgc cgtttaaagc cctgttcgct tcaggcggtg cgctgtatga
     4381 gtgggtgatc aacattccgg tgccgtacct cgataaactg gttgcccgta tgccgccagt
     4441 ggtcagcgaa gcgacagcgt atgccgccgt gtttaagttt gactggttct ctgccactgg
     4501 caccgccatt ctgtttgctg ccctgctctc gattgtctgg ctgaagatga aaccatctga
     4561 cgctatcagc accttcggca gcacgctgaa agaactggct ctgcctatct actccatcgg
     4621 tatggtgctg gcattcgcct ttatctcgaa ctattcagga ctgtcatcaa cactggcgct
     4681 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggcttgg
     4741 ggtattcctg accgggtcgg atacgtcatc taatgctttg tttgccgctt tgcaggctac
     4801 cgccgcacaa caaattggcg tttctgacct gttgctggtt gctgccaaca ccaccggtgg
     4861 tgtcaccggc aaaatgatct ctccgcaatc tatcgctatc gcctgtgcgg cggtaggtct
     4921 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     4981 tatggtgggc gtgatcacca cgcttcaggc ctatgtctta acgtggatga ttccttaatg
     5041 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     5101 gaaaaaaacc tggaagcggg catgaagttg cccgctgaac gtcaactggc gatgcaactc
     5161 ggcgtgtcga gaaattcact gcgcgaggcg ctggcgaaac tggtaagcga aggtgtgctg
     5221 ctcagtcgac gtggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     5281 aacatcgtcc agccgctgaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     5341 ctggaagccc gatacgccat tgaagccagc accgcatggc atgcagcaat gcgcgccaca
     5401 cctggcgaaa aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     5461 ctcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     5521 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctctgt gaagcatagc
     5581 cgccagcgga tgtacctggt acccccggtt ttttcacagc tgaccgaaca acatcaggcg
     5641 gtcattgacg ccatttttgc cggtgatgct gacggggcgc gtaaagcaat gatggcgcac
     5701 cttagctttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccatgcacgc
     5761 attacccgcc tgcctggtga ccataatgag cattcgaggg agaaaaacgc atgattattt
     5821 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     5881 atatggatgg gggggcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     5941 aagtggcgct gcgccagcgt attctgaaaa acatgtctga cttaagccta gaaacgacgc
     6001 tgtttaatga gaaactgtcg atgccggtgg cgctaggtcc ggtaggtttg tgtggcatgt
     6061 atgcgcgacg cggcgaagtt caggctgcca aagccgcgga tgcgcatggc attccgttta
     6121 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc tccagctatc aaacgtccga
     6181 tgtggttcca gctttatgtg ctgcgcgatc gcggctttat gcgtaacgca ctggagcgag
     6241 caaaagcagc gggttgttcg acactggttt tcaccgtgga tatgccaaca ccgggagcgc
     6301 gttatcgtga tgcgcattct gggatgagcg gcccgaacgc ggcaatgcgc cgctatttac
     6361 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccgcatgatt
     6421 taggtaatat ctcggcttac ctcggcaaac cgaccggact ggaagattac atcggttggc
     6481 tggccaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgacttct
     6541 gggatggccc gatggtaatc aaagggatac ttgatccgga agatgcgcgg gatgcagtac
     6601 gctttggcgc tgacggaatt gtggtttcta accacggcgg tcgtcagttg gacggtgtgc
     6661 tctcttctgc gcgtgccctg cctgctattg cagatgccgt gaaaggtgat atcgccattc
     6721 tggcagatag cgggattcgt aacgggctgg acgttgtgcg tatgatcgcg ctcggtgctg
     6781 ataccgtact gctgggccgc gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     6841 tagctaatct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     6901 cgaaatcgat tagcgaaatt acgcaagatt cgctggtgca ggtgctgggt aaagagttgc
     6961 cggcggcatt ggctccgatg gcgaaaggga atgcagctta agggttagac gaatatctgc
     7021 tatcctgccc ccgcactaag ggggcagtat gctaaacatc gtactttacg aaccagaaat
     7081 tccgccaaat actggcaaca tcatccgtct ttgcgctaat accggctttc gtctgcatat
     7141 catcgaaccg atgggatttg cctgggacga taagcgcctg cgccgcgcgg ggctggacta
     7201 tcacgagttt accgccgtta cgcgtcatca tgactatcgc gcgtttctcg aagcagaaaa
     7261 tccccagcgc ctgtttgccc tcaccacgaa aggtacgcct gctcacagcg ccgtaagcta
     7321 tcaggatggc gattatctga tgttcggccc ggaaacacgc ggcctgccag cgagcattct
     7381 tgatgccctg cccactgaac aaaaaattcg tattccaatg gtgccggaca gccgcagcat
     7441 gaacctgtcc aatgcggtgt cggtggtggt gtatgaagcc tggcgccagc tggggtatcc
     7501 gggagcggtg ttgagagatt aagatgcggt aggccggata aggcgttcac gccgcatccg
     7561 gcacgatcac agaatgtcag atcccatcac catactcaaa cgtatggtta atgccgttga
     7621 aatgctgatc catatccatt gacggtttat cgctatccgg cttgccaaca atgcgtgccg
     7681 gaacgccagc ggcagtagtg tgcggcggca ccggttgcag caccacggaa cctgcgccaa
     7741 tcttcgcgcc gcgcccaact tcaatattgc cgagaatttt cgcgcccgcg ccaatcatca
     7801 caccttcacg aattttcggg tgacggtcgc caccagattt acccgtaccg ccaagcgtca
     7861 cagattgcag aatcgatacg tcgttttcaa tcaccgccgt ttcacccaca acgatgcccg
     7921 tcgcgtggtc gagcatgata ccgcgaccaa tttttgccgc cgggtgaata tcaacctgga
     7981 acgttacaga gacctggttt tgtagaaaaa tagccagcgc gcgacggccc tgattccaca
     8041 accagtgacc gatgcgatac gcctgcaaag catgaaaacc cttcagatat agcaacgggg
     8101 ttgagtattt atccaccgcc gggtcacggg tacgcaccgc ctgaatatca caggccgcag
     8161 aggcgatcat ttccgggtcg gcggcgtagg cttcttccac cacttcacgg atagcaatag
     8221 ccggcataat gggcgatgac agcttgttcg ccagcatgta gctcagtgca ctgccaaggt
     8281 tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca
     8341 gcgttctggc ttcagcttta atattgttcc agacaatttc cagttcttca cacgacattg
     8401 cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagtggta
     8461 acaaaggttc cctgggttta gtggctgctg cgctcgtcct tgcgtgcacg acctagtaaa
     8521 gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt
     8581 attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag
     8641 ccttccacca cctgacctat cttttcctgc gcgctttgta catccatgcc ctgaccgagc
     8701 atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc
     8761 agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac
     8821 atttcagcca gtccacgggt gatcagcgcc gtacgcgcat tcgcgccaaa accgataccg
     8881 tcggacatcc ccgcgccaat ggcaatgacg tttttcaccg caccgccaag ctgcacaccg
     8941 atgaaatccg ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgctggagg
     9001 tcatcggcaa aggtctggtc cgtcgaggcc agcgaaatgg ccgtcggtaa acctgccgcc
     9061 agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc
     9121 tcacgcgcca cgtcctgtaa cagacgtccg gtttctgctt ccagcccttt ggtcgcccac
     9181 accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca
     9241 tggctgggta cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt
     9301 tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg
     9361 cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt
     9421 cttgccaggg tgatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa
     9481 gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct
     9541 gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg
     9601 taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc
     9661 agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac
     9721 cgccctgctg aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa
     9781 cacgcagtac cacttcgtaa acgtcatctg ccagttgagt agatgccgta tccagatcaa
     9841 gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag
     9901 aaatatcctt ggtataaata cgttggatct ggaaagtcat ttcggtgttg ttttgttctg
     9961 acatgtgtag aaaaccctta agtgttgtcc ttaaaatact gcgcaatgta atgccaacgt
    10021 tcacacgtta tttcagcagg ggatccagtc caccacgtgc atccaatgca tacaagtcat
    10081 cacagccacc aatgtgctgt gcgtcaataa aaatctgtgg aaccgtggtg cgaccgctgc
    10141 gtttgatcat ctcttcacgc ttagcggcat tgccatcgat cggcaactcc tgaaaactca
    10201 cgcccttgct gctcagcagt gcttttgcac gatggcaata cgggcaggtt tctttggtat
    10261 agatttcaac attggccata aattagctcc tgttttttac cctgaaattt catgttgcag
    10321 ggcggcagca aaatggagtg tccccagaag cttatatcaa taagtcgctg ggggcctaat
    10381 tgcagctaac ggcctgcatc atgaaaaacg gcaggtaaat tatttgccgc gcaccaaagg
    10441 caagttttcg ccagcccagc cagcgacgcc ttctttcagt acgaatactt gcgcaaaacc
    10501 agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccat ctaccacgat
    10561 aaccggtttg tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg
    10621 caacaggttg atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc
    10681 cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt
    10741 cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca
    10801 ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa
    10861 caactcccga cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc gttgcggcaa
    10921 atacagccag cgcgttaact ggaatgcaat tttgcggggc gctacgaaaa aaaagcgtaa
    10981 atctggcatc tgcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg
    11041 attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc
    11101 ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc gcgaagaaca
    11161 gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg
    11221 tccgcatacc ctgatcgacg cttccggttt ggaagtcggt ctgcctgacc gtcagatggg
    11281 caactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac
    11341 tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccggtgt tgactggtgc
    11401 agtagataaa gcgaaaaacg caggcaaagc agtacacatt atgggtctgc tctccgcagg
    11461 tggtgtacac agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg
    11521 cgcagaaaaa atctacctac acgcattcct tgacggtcgc gacactccgc cgcgcagtgc
    11581 tgaatcctcg ctgaaaaaat tcgaagagaa atttgccgcg ctgggtaaag gccgcgtagc
    11641 gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa
    11701 agcttatgac ctgctgactc tggcgcaagg tgagttccag gccgataccg ccgttgccgg
    11761 tttgcaggct gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg
    11821 tgcagaaggt cagccagatg cggcaatgga agacggcgat gcactgattt tcatgaactt
    11881 ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgta aatgccgatt ttgacggctt
    11941 cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg ctgccgacat
    12001 caaaaccgca gtggcttacc cacccgcttc cctggttaac accttcggcg aatggatggc
    12061 gaaaaacgac aaaactcagc tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt
    12121 cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc
    12181 accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa
    12241 actggttgcg gcaatcaaga gcggcaaata cgacaccatt atttgtaact atccgaacgg
    12301 cgacatggta ggtcacaccg gagtgatgga agcggcggtt aaagcggttg aagcgctgga
    12361 tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac
    12421 cgctgaccac ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacagc
    12481 acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttga
    12541 aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc
    12601 gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa
    12661 ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat
    12721 ctacgccagc gttctgagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga
    12781 gcgtgaccaa ctcaaatcca ttcaggctga catcgccgcg aaagagcgcg cggtacgcca
    12841 aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc
    12901 tgaagccacc cgtaaactgc gcgaaacgca aaacacgctt aatcaactga ataaacagat
    12961 tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag
    13021 cctcgccgcg caactggatg ccgcgtttcg tcagggtgaa cataccggta ttcagctgat
    13081 tctcagcggt gaagaaagcc agcgtgggca gcggttacag gcttatttcg gctatctcaa
    13141 ccaggcgcga caagaaacca ttgctcagtt gaaacaaacg cgtgaagaag tcgctatgca
    13201 gcgtgccgaa ctggaagaga aacagagcga gcaacaaacg cttttatatg agcagcgcgc
    13261 ccaacaggcg aagctgaccc aggcgttgag cgagcgtaaa aagacgctgg cagggctgga
    13321 gtcttccatc cagcaaggtc agcaacagtt gagcgagctg cgcgccaacg aatcccgcct
    13381 gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gtgaagcgcg
    13441 cgaagcccag gcggttcgcg accgccagaa agaagcgacg cgcaaaggca ccacctacaa
    13501 gccgaccgaa agcgaaaaat cgctgatgtc ccgtaccggt ggtctgggcg caccgcgcgg
    13561 tcaggcattc tggccagttc gcgggccaac gctgcatcgc tatggcgaac agctacaggg
    13621 tgaattacgc tggaaaggga tggtgattgg tgcctctgaa ggtactgagg ttaaagcgat
    13681 tgccgacggt cgggtgattc tggctgactg gctgcaaggc tacggtctgg tggtggtggt
    13741 tgagcatggt aaaggcgaca tgagtcttta cggctataat cagagcgcac tggtgagcgt
    13801 tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg
    13861 tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg
    13921 gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgccctgtt
    13981 ggcgctctcc tcccccgtaa ttgctggcaa acttgccatc gtcattgatg attttgggta
    14041 tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt
    14101 acccgattca ccgcacgcca gagagatggc gaccaaagca cataacagcg gtcacgaagt
    14161 gttgattcat ctaccgatgg caccactgag taaacagccg ctggagaaaa atacgctacg
    14221 cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc
    14281 ctatgccgtg gggatcaaca accacatggg cagcaagatg acctctaacc tgtttggtat
    14341 gcagaaagtg atgcaggcgc tggagcgtta caatctttac ttcctcgaca gcgtaaccat
    14401 cggtaatacc caggcgatgc gcgccgcgca aggcactggc gtgaaggtga tcaaacggaa
    14461 ggtgttcctc gacgattcgc aaaatgaagc ggacatccgt gtgcaattca atcgcgcaat
    14521 tgacctggcg cgtcgcaacg gttcaaccat tgccattgga catcctcacc cttcaacggt
    14581 acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag
    14641 cagcttgttg aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca ctgtgcccga
    14701 cacaccgcgt aatccattcc gtggcgtgaa gctgtgcaaa ccgaagaagc cgctggaacc
    14761 tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat
    14821 cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cccgaagcgg caaaacttaa
    14881 cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc
    14941 cattgccata agcggaaaga ccacagtaac gcctggtaac ccaccttcac gctgcgcaca
    15001 ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat ccgttggcgg
    15061 gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc gtaggttatt
    15121 tgctgatgaa attccggata aatcgtaatt ttgtcagcat aatttcgatt taatttctcc
    15181 agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttgtt cccttgtcta
    15241 tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg ctcggtatat
    15301 cgcgcacgca gcgcgttaaa cataaattct gttgtccaga caatatcctg atgatgtaat
    15361 ccggcaataa atctaatgtt atttttaaca ataacatcgc ggcgataaac ccccatccag
    15421 accacgtgtg tccaacggcg ggaagaaagt cccatccgca gccagtccgg accagttaat
    15481 acaccggttg agcgaaggcg atcggtaggg atggattgcc aggtttcacc cgtttcacga
    15541 aaacaccaat cagcgttgca ctgcgccacg tcgaggtcgt cctctaacgc catggtcatc
    15601 agcgtttcgt acatggtggg atagacctca tcgtcagcat cgacaaaagc gacatatttt
    15661 cccgttgcca cttcaatccc acgattacgc gccaccgatg cgccagcatt cgcctgatgc
    15721 aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac agaattatcc
    15781 gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt aattaaagat
    15841 tccatacaag tgaggaaatc atcacccgca ttatataacg gaataataac actaagttta
    15901 ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg ttttacaggc
    15961 tttgtattaa gaaactatta agcctgtgag atattatttt taccggtgag agagtcacgt
    16021 cagggtagag agataatgag agcagctata taagagatca gcgcaattac gatatcttga
    16081 ttacactgac aggattttac atatttaacg gattatcctc aggcaaaagg gctggaatac
    16141 cagcccttgt tcgtgttaat cccagctcag aataactttc ccggactggc ccgaacgcat
    16201 agcgtcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga tcggcgagag
    16261 atcgaggcca gactgaatca gcgccgccat cttgtaccag gtttcaaaca tctcacgacc
    16321 gtaaatacct ttaatgaaca agcctttaaa gattactttg gtccagtcga tagacatatc
    16381 ggacggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtgt cgagcatggt
    16441 acgaaacgct ggcggcgcac cggacatttc cagaccgaca tcaaagcctt cggtcatgcc
    16501 cagttctgtc atcacatcat taaggttttc tttagcgacg ttaaccgcac gggtgatacc
    16561 cattttgcgt gccagctcaa ggcggtattc gttaacatca gtgatcacca cattgcgtgc
    16621 accaacgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac cagagaccag
    16681 cacatcctcg ccaaccagat cgaacgacag cgcggtatgc acagcgttac cgaagggatc
    16741 aaaaatggaa gccaggtcgt cggagatatt gtccgggatt ttgaaggcgt tgaacgccgg
    16801 aatcaccaga tactcggcga agcagcccgg acggttaaca ccgacgccga tcgtgttgcg
    16861 gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga tatggccttc
    16921 gccagaaacg cgatcgccaa tcttgaagcc tttcacttcc tgaccaatac ctaccacttc
    16981 accgacatat tcatggccca cgaccatcgg caccgggatg gttttttgcg accactcatc
    17041 ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt taatcagcag
    17101 atcgttatgc ccgagttccg gtacaggaac gtcggtcatc cagatgccct cttccgcttt
    17161 cagtttggat aacgctttca tctcacaccc tcaggcgata acgcccagtt gtttaccaat
    17221 acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg cagacatctg
    17281 ggtacgaata cgcgcctgac ctttaggaac gaccggatag aagaaaccgg tgacgtaaat
    17341 gccctctttt tgcagctcac gggcaaattt ctgcgccact accgcatcgc caagcatgac
    17401 gggaataatg gcgtgatcgg ctcccgccag ggtaaagccc gccgccgaca tttgctcacg
    17461 gaactgacgc gcgttcgccc acagacggtc acgcagctcg ctacccgctt cgaccatctc
    17521 cagcactttg atggatgcgg cgacaatcgc tggtgccagc gagttggaga acaggtacgg
    17581 acgagaacgc tggcgcagcc actcaaccac ttctttgcgc gccgcggtat aaccaccaga
    17641 agccccgccc aacgctttac ccaacgtacc ggtaatgatg tctacacgtc ccatcacatc
    17701 gcagtattca tgggaaccac gaccattttc accgacaaaa ccgaccgcgt gggagtcgtc
    17761 taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca ggttggcaat
    17821 cacgccgtcc atcgagaaca caccatcggt ggcaatcagc acatgacgcg caccggcttc
    17881 acgcgcttct ttcagacgtg cttccagctc ctgcatatcg ttgttggcat agcgatagcg
    17941 tttagcttta cacagacgca caccatcaat aatagacgcg tggttcagtg cgtcggagat
    18001 aatggcgtct tccgcgctca gaagcgtttc aaacaggccg ccgttagcat caaagcagga
    18061 agagtagaga atcgcatctt ccatccccag gaatgccgcc agtttttgtt caagctcttt
    18121 atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac cgtgagaatc
    18181 cattcccgcc tttgccgccg caatcagatc cggatgattc gccagcccga gatagttgtt
    18241 ggcacaaaag ttaatgacgt ggcttccatc agccacagtg atatctgctt gctgcgcaga
    18301 cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca gatcgttggt
    18361 taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa ttccagcaca
    18421 tattacccaa acttataggt cgcgacgaga tgacgcgtta acacttctgc aaaattcagg
    18481 ataaataacg tgtatcttga tgtctcttaa cacctacaga tagtagaaaa actgtacagt
    18541 tttggctggg cataatgatg agtgatttta ctgcattgat ataaaaagaa aaaatgatgc
    18601 cagagcatca tttttccaat ctcatttggt atcaaaacgc tacccactaa tggagggaaa
    18661 cctattttct tgaaaggaaa attctcagcg cctgcatttt tgcccctaaa gtgttactcc
    18721 tgaagcagcg aaacaaatcg aaccattttc ctctccccag atagtttagg ttgaagagat
    18781 cggaacgttt gtaatagcac atgacaaaaa tgccctggtc atcgtccaca atgttattgt
    18841 ttagggttat tttttgactt tccagaacta atttatagaa ttctttccat ttatgctgtg
    18901 aaccaacaat tgcgccacca ataatatata catgattgcc aatcatgaaa tcaaaagcct
    18961 gttgttgaga tgtaacagca agcccttttt ttatagtgaa aagatgcatc ttattttcat
    19021 caaatgggaa atcccaaatt ttcaatcctc gagttacatt tggcttacgg caatagccaa
    19081 aatctatcca cgcaacaaga ggagttttca ctaagcccat gttgatagct ttatttacaa
    19141 aataggcctt aaggttacat accaatacat actctggtga ccagtactct gggtttttta
    19201 attgccgcgg ctcaagtcta tttgtaaacg actcatcttt ttgaattttt tcgatacgac
    19261 ttctgatata tctaaatttc ttttttatat cgataacgat aaccgttgtt ggttttccgt
    19321 tgcgaatcgc ttcaaccctt gatttcaaat cgggagaagt gaaaataatc atttcatttt
    19381 caagtgcggc cagacgctca aagtaactaa aataaacatc aactgaacga gctagttttt
    19441 cacggaaccc tttattagca gtccaatccc ctctaccaat gtcaaaatat gcagtgataa
    19501 ttgtggtgga tgatttcatt actaataaga ccattaatat aaaaaaagaa ttatatcata
    19561 aatattaaca gaatgtatac tgtatattat ccattcaaat gtttcagtta tttatcatac
    19621 atcggaatat tgatactaaa gcactattca cgtgcaaatc caacatccgc catgaaggac
    19681 tggctaaaac ccaaactagt ttgttgcaat tagcatcctt gcatctctat gtaaagggct
    19741 gaagggattc ggatgtgatg gtatgattac agacattcgt gtctgagatt gtctctgact
    19801 ccataattca aaggttacag ttatgatcat cgttaccggc ggcgcgggct ttatcggcag
    19861 caacatcgtt aaagccctga atgacaaagg catcaccgat attctggtgg tggataacct
    19921 gaaagacggc accaagtttg tgaacctggt ggatctggat atcgcggact atatggataa
    19981 ggaagacttc ctgatccaga ttatggctgg cgaagagttc ggcgatgtcg aggcgatttt
    20041 ccacgaaggc gcgtgctctt ccaccaccga gtgggacggc aagtatatga tggataacaa
    20101 ctatcaatac tccaaagagc tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta
    20161 tgcctcttcc gcagccactt acggtggacg cacctccgac tttattgaat cccgcgaata
    20221 tgaaaaaccg ttgaatgtct acggttactc aaaattcctg tttgatgaat atgttcgcca
    20281 aatcctgcct gaagcgaact cacagattgt tggcttccgt tatttcaacg tttatggacc
    20341 gcgtgaaggc cataaaggca gcatggcgag cgtcgctttc catctcaaca cccagcttaa
    20401 taacggtgaa tcaccgaagc tgttcgaagg tagcgagaac ttcaaacgtg acttcgtcta
    20461 tgtgggcgat gtggcagatg taaacctgtg gttcctggaa aatggcgttt ccggcatctt
    20521 caacctcggc accggtcgtg cggaatcctt ccaggcagta gcagatgcta cgcttgctta
    20581 tcacaagaaa ggccagatcg aatacattcc gttcccggat aaactgaaag gccgctacca
    20641 ggcgttcact caggcagatc tgacgaatct gcgcgcggcg ggttacgata aaccgttcaa
    20701 aaccgttgcc gaaggcgtaa cggaatacat ggcctggctg aatcgtgacg cataagagct
    20761 ctgcatgaaa atactggtga tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag
    20821 tctctatcgc acgctccagg cgcgctatcc ccaggcgata atcgacgtga tggcaccggc
    20881 atggtgccgt ccattattat cgcggatgcc ggaagttaac gaagcgatcc ctatgcctct
    20941 cggtcacgga gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagtc tgcgtgaaaa
    21001 gcgctacgac cgcgcctacg tcttacctaa ctccttcaaa tccgcattag tgcctttctt
    21061 cgcaggtatt cctcatcgca ccggctggcg cggcgagatg cgctacggtt tactcaacga
    21121 tgtacgcgtg ctcgataaag aagcctggcc gctaatggtg gaacgctata tcgcgctggc
    21181 ctatgacaaa ggcgttatgc gtaccgcaca agatctgccg cagccattgt tatggccgca
    21241 gttgcaggtg agcgaaggtg aaaaatcata tacctgtaat caattttcgc tctcatcaga
    21301 acgtccgatg attggttttt gcccgggtgc ggagtttggt ccggcaaaac gctggccaca
    21361 ctaccactat gcggagctgg caaagcagct gattgatgaa ggttatcagg tggttctgtt
    21421 tggctcggcg aaagatcatg aagcgggcaa tcagattctt gccgctttga ataccgagca
    21481 gcaggcatgg tgtcggaatc tggcgggaga aacacagctt gatcaagcgg ttatcctgat
    21541 tgcagcctgt aaagccattg ttactaacga ttctggtctg atgcacgttg cggcggcgct
    21601 caatcgtccg ctggttgccc tgtatggacc aagcagcccg gacttcacac caccgctatc
    21661 ccataaagca cgcgtgatcc gcctgattac cggctatcac aaagtgcgta aaggtgacgc
    21721 tgcggagggt tatcaccaga gcttgatcga cattactccc cagcgcgtac tggaagaact
    21781 caacgcgcta ttgttacaag aggaagcctg acggatgcgg gttctgatcg ttaaaacatc
    21841 gtcgatgggc gatgttctcc acacgctgcc cgcactcact gatgcccagc aggcaatccc
    21901 agggattaag tttgactggg tggtggaaga agggttcgca cagattcctt cctggcacgc
    21961 tgccgttgag cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc
    22021 tcccataaaa gctgaacgca aagcgtttcg tgaagcgcta caagcaaaga actatgacgc
    22081 agttatcgac gcccaggggc tggtaaaaag cgcggcgctg gtgacgcgtc tggcgcatgg
    22141 cgtaaagcat ggaatggact ggcaaaccgc tcgcgaaccg ttagccagcc tgttttacaa
    22201 tcgtaaacat catattgcaa aacagcagca cgccgtagaa cgcacccgcg aactgtttgc
    22261 caaaagtctg ggctatagca aaccacaaac ccagggcgat tatgctatcg cacagcattt
    22321 cctgacgaac ctgcctacag atgctggcga atatgccgta tttcttcatg caacaacccg
    22381 agatgataaa cactggccgg aagaacactg gcgagaattg attggtttac tggctgattc
    22441 aggaatacgg attaaacttc cgtggggcgc gccgcatgag gaagaacggg cgaaacgact
    22501 ggcggaagga tttgcttatg ttgaagtatt gccgaagatg agtctggaag gcgttgcccg
    22561 cgtactggct ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc atttaacggc
    22621 ggcactggat agacccaata tcacggttta tggacctacc gatccgggat taattggtgg
    22681 gtatgggaag aatcaagtag agtgtcgttc tacaagtatg tctcttgcag atttgccagc
    22741 tcaaaccgtt tttcaaaatt taaatcttga aataataatc aataagttga catcggagat
    22801 aagatgacct caacattatt tttctctttc gagaaaaaaa actggatagc gtattggaac
    22861 agagctcttg tattcttatt cattaccacc tattttttgg gtgggattac aaggtataaa
    22921 catcttattg ttattcttat gacaataacg acaatcgtct atctctgcaa acggccaaaa
    22981 cattatctat cattatttaa aacatttctt tttggtagtg ttgccatatt aactattgct
    23041 gcattgctat cacttgttca atccccagat gcaggtgctg gcatgaagga aattttcaaa
    23101 gctatcattg aaaatacttt gctatgcaca atagcaatac cggttatatt gagagacgag
    23161 aaaagagaag atatcgaaaa aatcgttttc ttctcattta ttagtgcgtt aggcttacgc
    23221 tgtttttctg aattgattgc ctattataag gactatcaac aagggataat gccattcgca
    23281 gattatagac accgtagcat ttctgactcg atggtttttt tattccctgc attgttaaat
    23341 ctctggctta tcaaatcagc aaaataccgc atttcttttg cggttctaag cgctattttt
    23401 atttttctga tattaggaac tttatccaga ggggcctggc tttccgtgtt agtcattgga
    23461 ttaatatgga ttctgatgtt taaacaatgg aagttactat tagtaggagt aatggtttcc
    23521 atcattgcat tgtcggttat tttcacacat aaggagatga ccgcaaagct aacgtataaa
    23581 cttcaacaaa ctaatagttc ttatcgttat gcaaatggta ctcaaggcag cgcactcgat
    23641 ctaatattag aaaatcctgt tattggttac ggttacggta acgttgcata taaagatgtc
    23701 tataataaac gagtcattga ttatccgcaa tggaccttta gacaatcgat agggccacat
    23761 aattttgcgc tattcatctg gtttggcact ggtttattag ggctggtaag tcttatgatg
    23821 ctatactgtg caatactaaa agagtgtata aaaaatgtcg tccagaataa ataccgctca
    23881 ccatata
//
LOCUS       DADTFG010000073        23680 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.073, whole genome shotgun sequence.
ACCESSION   DADTFG010000073 DADTFG010000000
VERSION     DADTFG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.073"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            38..991
                     /gene="ompT"
                     /locus_tag="J1138_002633"
     CDS             38..991
                     /gene="ompT"
                     /locus_tag="J1138_002633"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HBA8454409.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEAFDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRV
                     TNKKGDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1235..1891
                     /locus_tag="J1138_002634"
     CDS             1235..1891
                     /locus_tag="J1138_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA8454410.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPH
                     FLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHD
                     VFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDG
                     VVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSASGKK
                     "
     gene            complement(1946..2251)
                     /locus_tag="J1138_002635"
     CDS             complement(1946..2251)
                     /locus_tag="J1138_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA8454411.1"
                     /translation="MVVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVT
                     WLSPAGEFQKWNGTSWIKDTEEETSLLEACKMYRVLLNRVDTSTAPDIEWPVNPVRE"
     gene            2333..2938
                     /locus_tag="J1138_002636"
                     /pseudo
     CDS             2333..2938
                     /locus_tag="J1138_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
     gene            complement(2946..3022)
                     /locus_tag="J1138_002637"
     tRNA            complement(2946..3022)
                     /locus_tag="J1138_002637"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2986..2988),aa:Arg,seq:tct)
     gene            complement(3055..3252)
                     /locus_tag="J1138_002638"
     CDS             complement(3055..3252)
                     /locus_tag="J1138_002638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454412.1"
                     /translation="MSILSEAWRAVSFRFLKSKTFCTWLIHTYHDNRRWFAAIVSYIR
                     CGRTLVQQLVQKPLTQQGLTV"
     gene            3293..4159
                     /gene="folD"
                     /locus_tag="J1138_002639"
     CDS             3293..4159
                     /gene="folD"
                     /locus_tag="J1138_002639"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBA8454413.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAVKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            4161..4373
                     /gene="ybcJ"
                     /locus_tag="J1138_002640"
     CDS             4161..4373
                     /gene="ybcJ"
                     /locus_tag="J1138_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBA8454414.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            4481..4954
                     /locus_tag="J1138_002641"
     CDS             4481..4954
                     /locus_tag="J1138_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA8454415.1"
                     /translation="MPTVITHAAVLLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWV"
     gene            complement(5038..6423)
                     /gene="cysS"
                     /locus_tag="J1138_002642"
     CDS             complement(5038..6423)
                     /gene="cysS"
                     /locus_tag="J1138_002642"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBA8454416.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDGEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            6597..7091
                     /gene="ppiB"
                     /locus_tag="J1138_002643"
     CDS             6597..7091
                     /gene="ppiB"
                     /locus_tag="J1138_002643"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBA8454417.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            7094..7816
                     /gene="lpxH"
                     /locus_tag="J1138_002644"
     CDS             7094..7816
                     /gene="lpxH"
                     /locus_tag="J1138_002644"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBA8454418.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7927..8256
                     /locus_tag="J1138_002645"
     CDS             7927..8256
                     /locus_tag="J1138_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454419.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERFILIVGICFFPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            8352..8861
                     /gene="purE"
                     /locus_tag="J1138_002646"
     CDS             8352..8861
                     /gene="purE"
                     /locus_tag="J1138_002646"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBA8454420.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNEWRKAQTDEVL
                     ENPDPRGAA"
     gene            8858..9925
                     /gene="purK"
                     /locus_tag="J1138_002647"
     CDS             8858..9925
                     /gene="purK"
                     /locus_tag="J1138_002647"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBA8454421.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLSE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELS
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(10058..10951)
                     /locus_tag="J1138_002648"
     CDS             complement(10058..10951)
                     /locus_tag="J1138_002648"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA8454422.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(10948..11763)
                     /locus_tag="J1138_002649"
     CDS             complement(10948..11763)
                     /locus_tag="J1138_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA8454423.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFAFSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(11774..13033)
                     /locus_tag="J1138_002650"
     CDS             complement(11774..13033)
                     /locus_tag="J1138_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA8454424.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(13043..14710)
                     /locus_tag="J1138_002651"
     CDS             complement(13043..14710)
                     /locus_tag="J1138_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA8454425.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            15027..16076
                     /gene="allD"
                     /locus_tag="J1138_002652"
     CDS             15027..16076
                     /gene="allD"
                     /locus_tag="J1138_002652"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HBA8454426.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            16098..17333
                     /gene="allC"
                     /locus_tag="J1138_002653"
     CDS             16098..17333
                     /gene="allC"
                     /locus_tag="J1138_002653"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBA8454427.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            17344..18129
                     /gene="allE"
                     /locus_tag="J1138_002654"
     CDS             17344..18129
                     /gene="allE"
                     /locus_tag="J1138_002654"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBA8454428.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFT
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(18258..19403)
                     /gene="glxK"
                     /locus_tag="J1138_002655"
     CDS             complement(18258..19403)
                     /gene="glxK"
                     /locus_tag="J1138_002655"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HBA8454429.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELILHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(19425..20726)
                     /locus_tag="J1138_002656"
     CDS             complement(19425..20726)
                     /locus_tag="J1138_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBA8454430.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(20782..22143)
                     /gene="allB"
                     /locus_tag="J1138_002657"
     CDS             complement(20782..22143)
                     /gene="allB"
                     /locus_tag="J1138_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HBA8454431.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(22203..23657)
                     /locus_tag="J1138_002658"
     CDS             complement(22203..23657)
                     /locus_tag="J1138_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HBA8454432.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         5953 a         6317 c         5883 g         5527 t
ORIGIN      
        1 atactcaatc attaaaacga ttgaatggag accttttatg cgggcgaaac ttctgggaat
       61 agtcctgaca acccctattg cgatcagctc ttttgcttct accgagactt tatcgtttac
      121 tcctgacaac ataaatgcgg acattagtct tggaactctg agcggaaaaa caaaagagcg
      181 tgtttatcta gccgaagaag gaggccgaaa agtcagtcaa cttgactgga aattcaataa
      241 cgctgcaatt attaaaggtg caattaattg ggatttgatg ccccagatat ctatcggggc
      301 tgctggctgg acaacactcg gcagccgagg tggcaatatg gtcgatcagg actggatgga
      361 ttccagtaac cccggaacct ggacggatga aagtagacac cctgatacac aactcaatta
      421 tgccaacgaa tttgatctga atatcaaagg ctggctcctc aacgaaccca attaccgcct
      481 gggactcatg gccggatatc aggaaagccg ttatagcttt acagccagag gtggttccta
      541 tatctacagt tctgaggagg gattcagaga tgatatcggc tccttcccga atggagaaag
      601 agcaatcggc tacaaacaac gttttaaaat gccctacatt ggcttgactg gaagttatcg
      661 ttatgaagat tttgagctag gtggtacatt taaatacagc ggctgggtgg aagcatttga
      721 taacgatgaa cactatgacc caggaaaaag aatcacttat cgcagtaaag tcaaagacca
      781 aaattactat tctgttgcag tcaatgcagg ttattacgta acgcctaacg caaaagttta
      841 tattgaaggc gcatggaatc gggttacgaa taaaaaaggt gatacttcac tttatgatca
      901 caatgataac acttctgact acagcaaaaa tggtgcaggc atagaaaact ataacttcat
      961 cactactgct ggtcttaagt acacctttta acaacgttaa atagattttc tccccggtgg
     1021 tattgtatag ccccggggaa tttgtttacc gttaaaagca aacaatatcc cctaatattg
     1081 taagcattta acctgcgtcc tggcgtgtaa cctcttaccg caacacaaga cccaatatat
     1141 tcaaattcgt tatgccgtgg catcatttaa ccccttgtaa ttcatcgcta taattgattt
     1201 aattcacaaa taaaactata acacggtgaa actaatgaaa aaaaacacag atgatggggc
     1261 taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa
     1321 tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacattt
     1381 aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc acgtacctga
     1441 gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catcgacaag
     1501 ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgatc cttatcccgc
     1561 ggcattacat ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg
     1621 aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgacgatgg
     1681 aactctatac ggagccacaa ttcttggtga cggcgttgtg cacaatagct tcggtcaaaa
     1741 actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg
     1801 cttaacacat atactctcag agcatttcga gaatgttaaa accaaggtta aaggtactgt
     1861 agtaatgttt tccgcttcag ggaaaaaata gcatccagcc gcagcacgtt cttgcgcacg
     1921 acgtgctgcg gcataatccc aatgattact ctctgacagg gttcacaggc cactcaatat
     1981 caggggcagt tgatgtatca actcggttca gcaacacccg atacatctta caggcttcca
     2041 gcaacgaggt ttcttcctcc gtatccttta tccatgatgt gccgttccac ttctgaaact
     2101 cccctgccgg ggataaccag gtgacatttt ccggtaacgg accgagttca gaaataaata
     2161 acgcgtcccc tgatgccacg tcataaaccg ttttaccccg atggtcttca acgagatgcc
     2221 acgatgcctc atcactgttg aaaacaacca cgaagccagc cggaatatct ggcggtgcaa
     2281 tatcggtact gtttgcaggc aaacctgtat gaggtggaat ggcacaagag acttacaaaa
     2341 gaatcaggca aaacgcctga ttgatgagag ccccgaacca ctgaaatctg tcgtcaaatt
     2401 tgcgctggta actgttctga gaaagtcgaa catcataaat ctggagtggc aacagattga
     2461 catgcagcga caagttgcca gggtgaatcc agaagatagc aaatcaaacc gcgccattgg
     2521 tgtggcgctg aacgataccg cctgtaaagt gttgcgtgat caaataggca agcatcacaa
     2581 atgggtgttt gtacatacca aggcggctaa gcgagcagat ggaacatcaa cgcctgcggt
     2641 caggaagatg cgcatcgaca gcaagacatc atggctatca gcttgtcgtc gtgcaggaat
     2701 tgaagatttc cgtttccatg acctcagaca cacctgggca agctggctga ttcagtcagg
     2761 cgtcccatta tcagtgcttc aggaaatggg cggatgggag tccagagaaa tggttcgtag
     2821 atatgctcac cttgcgccta atcatttgac tgagcacgcg aggaaaatag acgacatttt
     2881 tggtgataat gtcccactct ggaattatag aagaaataaa gaaggcgtaa ctgattgaat
     2941 tttaatggcg cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc
     3001 tatccaactg agctaagggc gcattgatac cacggtgtgc tgtaatcgcg tgaattatac
     3061 ggtcaaccct tgctgagtca atggcttttg aaccagttgc tgaacaagtg tacgaccgca
     3121 tctgatgtag ctcactatgg cggcaaacca acgacggtta tcatgatagg tgtgaatcag
     3181 ccaggtacag aaggttttac ttttcagaaa acgaaaactg acagcgcgcc acgcttctga
     3241 caaaatagac acatcccctt cgacctacgt aacagatgga atcctctctc tgatggcagc
     3301 aaagattatt gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt
     3361 tcaggcgcgt attgcagccg gactgcgggc accaggactg gccgttgtgc tggtgggcag
     3421 taaccctgca tcgcaaattt atgtcgcaag caaacgcaag gcttgtgaag aagtcgggtt
     3481 cgtctcccgc tcttatgacc tcccggaaac caccagcgaa gcggagctgc tggagcttat
     3541 cgatgcgctg aatgccgaca acaccattga tggcattctg gttcaactgc cgttaccggc
     3601 gggtattgat aacgtcaaag tgctggaacg tattcatccg gacaaagacg tggacggttt
     3661 ccatccttac aacgtcggtc gtctgtgcca gcgcgcgcca cgtctgcgtc cctgtacccc
     3721 gcgcggtatc gtcacgctgc ttgagcgtta caacattgat accttcggcc ttaacgccgt
     3781 ggtgattggc gcatcgaata tcgtcggtcg cccaatgagc atggaactgc tgctggcagg
     3841 ttgcaccacc accgtgactc accgtttcac taaaaatctg cgtcaccacg tagaaaatgc
     3901 cgatctactg atcgttgccg tcggcaagcc gggctttatt cctggtgact ggatcaaaga
     3961 aggcgcaatt gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga
     4021 cgtcgtgttt gaagacgcgg ttaaacgcgc ctcatacatt acgcctgttc cgggcggcgt
     4081 tggcccgatg acggttgcca cgctgattga aaacacgcta caggcgtgcg ttgaatatca
     4141 tgatccacag ggtgagtaac atggcgacat tttctttagg taaacatccg cacgttgagc
     4201 tgtgcgactt gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga
     4261 tagcagaagg ccaggtgaaa gtcgacggtg cggttgaaac gcgtaaacgc tgcaaaatcg
     4321 tcgccggtca gacagtgagt tttgcaggtc acagcgtaca ggttgttgcc tgatcccttt
     4381 cccggcctgt atcaacaggc cggttctctt ttcagacttt tgccctctac gccccgttca
     4441 tccatgctga tagcatcggc attttgccga agcgaccatc atgccaaccg ttattacgca
     4501 cgccgccgtt ctcctatgca ttggtttagg gttgggttcg aaagccatcc ctccgcgtct
     4561 gttatttgcc ggaatcatcc tggcgatgct gcccgatgct gatgtattgt cgtttaaatt
     4621 tggcgttgct tacggcaatg tttttggtca tcgcgggttt acccattcgc tggtgtttgc
     4681 gtttgttgtc ccgctgttat gtgtgctgat tggacgtcga tggttcaggg ctgggctgat
     4741 tcgctgctgg ctgtttttaa ccgtctcgtt gctatcgcac agcttgctgg attcggtaac
     4801 cactggcggt aaaggggttg gctggctgtg gccgtggtca gatgaacgct ttttcgctcc
     4861 ctggcaggtg attaaagtcg cgccgtttgc attgtctcgt tacaccacgc cgtacgggca
     4921 tcaggtgatt atttctgaat tgatgtgggt gtagttgccg gggatgctac tgatgggaat
     4981 gttgtggtgg cgcaggcgat aaccggatgc gaaaactcgc atccggcaat agcgcaatta
     5041 cttacgacgc caggtggtcc cttgcgggcc atcttccagc acgatcccca tctcgttaag
     5101 gcggtcacgc gccgcatctg ccgccgccca gtctttcgct ttacgggcat ccagacgctg
     5161 ttgaattaac gcttcaatct cagccacttc gccgtcgtct gcctgcgcac cgctttgcag
     5221 gaacgcttcc ggttcttgct ccagcaggcc caatacggca gaaagtttac gcaggtgaga
     5281 cgccattgca ttcgccgctg ccatatcttc tgctttcaga cggttcactt cacgcgccat
     5341 atcaaacagc acggaatagg cttccggggt gttgaaatcg tcatccattg cttcaataaa
     5401 gcgcgcttca aacgcttcgc caccggcagg cgcaacggtt ttgtctgtgc cgcgcaaggc
     5461 agtatagaga cgttccagcg ccgcacgcgc ctgtttcagg ttctcttcgc tgtaattcaa
     5521 ctgactgcga tagtgacccg acatcaggaa gtaacgcaca gtttccgcat cgtagtattt
     5581 cagcacgtca cgcacggtga agaagttacc cagcgattta gacatcttct cgcggtcaac
     5641 catcaccata ccggagtgca tccagtaatt cacatactgg ccatcgtgcg cacaggtgga
     5701 ctgggcaatt tcgttctcgt ggtgcgggaa catcaggtca gaaccgccgc cgtggatatc
     5761 aaagtggtta cccaactgtt tgcagttcat tgccgaacac tcaatgtgcc agccaggacg
     5821 gcccgcgccc cacggagacg gccagctcgg ttcgccctct ttcgacatct tccacagaac
     5881 gaagtccatc gggttgcgtt tgtcgtcgac cacgtcaacg cgcgcgcctg cctgcagctg
     5941 atcgagatcc tgacgcgaca gcacgccata tgtcggatcg gtcgggacgt cgaacatcac
     6001 gtcgccgttg tccgccacat aagcgtgacc tttggcgatc agttgttcag tgagttcaat
     6061 aatttcggcg atatggtgcg tcgcgcgcgg ctccatatcc gggcgcagaa tgttcaaagc
     6121 atcaaaatct ttgtgcattt cggcgatcat gcgatccacc agcgccacaa agctttcgcc
     6181 attttcattg gcgcgtttga tgattttgtc gtcgatatca gtaatgttgc gcacatactt
     6241 cagcttatag ccgaggaaac gcagatagcg cgcaaccacg tcaaaggcaa caaaggtacg
     6301 cccgtgaccg atatgacaga gatcgtaaac ggtgattcca cacacgtaca tgccgacttc
     6361 cccggcgtga ataggcttaa attcctcttt ttggcgtgtc agagtattga agatttttag
     6421 catcgaagat tccgtttaga catgtgtggg taattgagtt gcgtataata cccatatttc
     6481 ccgcgcgaat cagcatacat tgcgtgatga tccgatcgtg cggttatgct ataacaccac
     6541 cctatatatg acccgaaccg ggttgaagca ccaatcaaac ggaacaggat gcaaaaatgg
     6601 ttactttcca caccaatcac ggcgatattg tcatcaaaac ttttgacgat aaagcacctg
     6661 aaacagttaa aaacttcctg gactactgcc gcgaaggttt ttacaacaac accattttcc
     6721 accgtgttat caacggcttt atgattcagg gcggcggttt tgaaccgggc atgaagcaaa
     6781 aaaccaccaa agacccgatc aaaaacgaag cgaacaacgg tctgaaaaat acccgtggta
     6841 cgctggcaat ggcccgtact caggcaccgc actctgcaac tgcccagttc ttcatcaacg
     6901 tggttgataa cgacttcctg aatttctctg gcgaaagcct gcaaggttgg ggctactgcg
     6961 tatttgctga agtggttgaa ggcatggacg tggtagacaa aatcaaaggt gttgcaaccg
     7021 gtcgtagcgg tatgcaccag gacgtgccaa aagaagacgt tatcattgaa agcgtgaccg
     7081 ttagcgagta atcgtggcga cactctttat tgcagatctt catctctgcg cggaagaacc
     7141 ggcgatcacc gccggttttc tgcgtttttt agcgggggaa gcccgcaagg ccgacgcgct
     7201 gtatattctt ggcgatctgt ttgaagcgtg gattggcgac gacgatccca acccactcca
     7261 tcgccagatg gcggcagcga tcaaagcggt atccgattcc ggcgttccct gttatttcat
     7321 tcatggcaac cgtgattttc tgctcggcaa acgctttgcc cgtgaaagcg gcatgacgtt
     7381 attgccggaa gaaaaggtgc tcgaacttta tggtcgtcgg gtgttgatta tgcatggcga
     7441 cacgctgtgc accgatgacg cgggttatca ggcttttcgc gccaaagtcc acaaaccctg
     7501 gctgcaaacg ctatttctcg ccctgccgtt gtttgtgcgc aaacgcattg ccgcgcgaat
     7561 gcgcgcgaac agcaaagaag ccaacagcag taaatcgctg gcgatcatgg acgttaacca
     7621 aaacgcggtg gtcagtgcga tggaaaaaca tcaagtgcaa tggctgatcc acgggcatac
     7681 ccatcgcccg gcggtgcatg aacttatcgc caatcagcaa cctgcttttc gcgtggtact
     7741 gggtgcctgg catacagaag gttcgatggt gaaagtcacg gcggatgacg ttgagctgat
     7801 tcattttcct ttctgaccac gaatggacag attctgaaaa agcaccgaac taacgcaacc
     7861 tgtatatctt ttttaaaatc agcaaggtaa taatctctga gtgaaataac aacagggaat
     7921 attattatgc aacgctgcaa cggatttttg atttctggca tcgcccttac ggcaggagcg
     7981 ccactgtgta ttagctggtt cggtcttgtc ggcttctatt ttggcgcatt gtcactggta
     8041 gctgcggcgg tcatttcctg gatagtctat ttctggctca tttatttagt actcacccgc
     8101 ccccataacc aacgttttgt agaaagattt atattgattg tcggtatttg cttttttcca
     8161 tggagcattg tgttgatatg ggcattcttt gccagttcag ccagagcgca taatcaacac
     8221 gcagaacaaa ttaaaaactc aacaactgac ggttaattcc gcctacgcaa ccgttttcct
     8281 tgcactcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag
     8341 ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca
     8401 aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg aatgtcccgc
     8461 accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa
     8521 gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc
     8581 ctggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg
     8641 cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg
     8701 gtacgctggc gattggtaaa gctggcgcgg caaacgcggc gttactggca gcacaaattc
     8761 ttgcgactca tgataaagaa ctgcaccagc gtctgaatga atggcgcaaa gcacaaactg
     8821 atgaagtgct ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg
     8881 taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg
     8941 gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc
     9001 tgagatcgaa cgctggccgg aaactgcatt aacccgcgag ctggcgcgcc atccggcctt
     9061 tgtgaaccgc gatgtgttcc cgattattgc cgaccgtctg actcagaagc agcttttcga
     9121 taagctccac ctgccgaccg caccgtggca gttactttcc gaacgcagcg agtggcctgc
     9181 ggtgtttgat cgtttaggtg agctggcgat tgttaagcgc cgcactggtg gctatgacgg
     9241 acgcggtcaa tggcgtttac gcgccgatga aaccgaacag ttacctgctg aatgctacgg
     9301 cgaatgtatt gtcgagcagg gcataaactt ctctggtgaa gtgtcgctgg ttggcgcgcg
     9361 cggttttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggcat
     9421 tttgcgcacc agcgtcgctt tcccgcaggc caacgcacag ctgcaggcgc aagctgaaga
     9481 gatgttgtcg gcgattatgc aggagctgag ctatgtaggc gtgatggcaa tggagtgttt
     9541 tgtcacccca caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca
     9601 ctggacgcaa aacggtgcca gcatcagcca gtttgagcta catctgcggg caattaccga
     9661 tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag
     9721 cgatctgaat tatgactggc tgaaactgcc gcttgtgcat ctgcactggt acgacaaaga
     9781 agtccgtccg ggccgtaaag tggggcattt gaatttgacc gacagcgaca catcgcgtct
     9841 gaccgcgacg ctggaagcct taatcccact gctgccgccg gagtatgcca gcggcgtgat
     9901 gtgggcgcag agcaagttca gttaattgat gctcattttc ttgccggatg cggcgtaaac
     9961 gccttatccg gcctaccgtt ccgacaccgc ctgtagacct gataagacgc gccagcatcg
    10021 catcaggcta gatgtgccaa ttgcctggtg cccatcacta cagcgaaata caagtccccg
    10081 cttcgcccgc cagcgtctct tcaatccgcg ataacgcccc aatccacgcg ggtttaccac
    10141 ggcttctgac atagccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
    10201 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
    10261 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
    10321 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccgtctt
    10381 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
    10441 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgta
    10501 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
    10561 ctggctgata aaccggacca ataaatttct ctggctgtaa aaacgccgga tcatcaggcg
    10621 aaacctcaat gcgcgtcagc accgtcgtga caggcggcat ctgcggctgt gcgctcaagc
    10681 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
    10741 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggtccgt
    10801 tgccgtgaac aatcgccagc cgataagaac gggccaggcg cgccagcgcg ggtacagcac
    10861 tggcaatatt gcgatattga ttttctgccg tcagcgcctc accgcgctgg agtaaggcgt
    10921 tgcccccaag agccacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
    10981 ccagaacccc agcagcgtat cggcccctga cgtatgcccc agcgccagca gcgaatcgat
    11041 cgccgctgcg gtacgtttcg gacaactcag agcatgaaca aagtgcagga gtggcgaggc
    11101 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgcagtgg taacgagttg
    11161 aagattgtcg gaaaaggcaa aaaacgggcg accggagcgc gcatctaaag ccccataata
    11221 ccaggccgcc agcagcattc cgctcagcgt gtcatcatga cttggcgtta atccggggcc
    11281 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    11341 acgaaactgg cgtaattcag caggcagcgg atcgcttgcc gccaatgcca gtggcccgaa
    11401 aagcccggtt tcctccgcgc gttgcatcca tgcaacggca agcggttgag gatgcgcagg
    11461 cggcgtaata cgtagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    11521 aatcccttgt gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    11581 gaattgcgcg cggcgaagca cccatccgcc ggggccgaaa ccactcccct gacgatgcag
    11641 cgttaacagt tcgccgcttt ccgtcatcag gttaatcgcc cgccgccaca ctcccgctaa
    11701 ccgccaggac tggcgataat tcggtgcgct acggctggca agtagaggat ggatgatcgt
    11761 cattgtgcgt tcctcaacca atccccatgc tttcagccag tgcttccagc gcctgttcaa
    11821 agcacgccag cggtgcccgc acggtgcctg cgccaatctg cccgatcccc gcctctttat
    11881 gggcgatacc ggtattgatg agtggcgtaa tgccggtttc taccacgcga cgaatgtcca
    11941 gccccaggca cgccccctgg aaatcccagc cggggatctg caattgcata ttgcgttcaa
    12001 ggtaaatttc cgccatctct tcagataccg ctctggccgc ttccatgcca cccgcaccga
    12061 caaagcgcgt tacgccaggt gcggcgatca ttgccgcacc tccgatacca aaggtttcgg
    12121 taatcgcgct atcgcccata tccgggttcg cctgctcctg cgagaagccg gtgaaaaaca
    12181 gaccttgcgg agtgtttaca ggcgcagtaa accagcgttc acccagcccg cttacccgaa
    12241 tcccgaacat attgccgttg cgggtcatag ccgtaacgat gctgcctgcg cggatcatcg
    12301 cgcctgcatc catcgccgcc ttgcagtagg ccatcgcgag attgaggaag aactgatcgg
    12361 tcacgctgag gaaatccatc acttcggcga tgtgctgttt atcatgatcg aggcgagcaa
    12421 tttgtggggc cagcgcacgc attaacagtg cagaggaagc aatattgcgt tgatggaact
    12481 catcgcccat ggtaatgccc tgcgccatca ttgccgtgag atcgataccg cgctccatgc
    12541 gccccagcgc cgcgcttaat actggcatta acacatcgcg catccagcga tggcgagtca
    12601 ggacatcttc gccgtaagcg ccaaaacgca tcactttgcc gataccttcg ttgaggttgc
    12661 agtacgcccg gttgccgtcg gtcacgttct cgaccaccag catcggcata ctggcagaag
    12721 taatgccacc cattggcccg acggcattca catggtgaca aggaatgaag ttcacttccc
    12781 cctgctccag tattgccagc gcctgcgctt catctttcgc ccagccttcg aacagacatg
    12841 cgcccacgca cgcccctttc atgggtccgg tcatctcctg ccagcgcatt ggcggcccgg
    12901 cgtgaagcag tgttttgccc ttgtttagtt cgctgataag tgaagaagcc ggttgcacat
    12961 ccagccagtg tggacgagcg cgacgaattt gttcgataac cgcagcattg gcttgcgcca
    13021 ctgatgtaaa catgggaacc ccttattgca aacgttctaa taaacgagcc agttttttat
    13081 tgccgccggc gactggcgac cattggtaat gcacaaccgg tttgctggcg ctttgtagct
    13141 ccagcgcaaa gctgcgtaat ccgatgttaa tcacggcgac gttttccagt aatgacggtg
    13201 tgtgttgctg tgtggctgac gagagcggat gaattaacgc cgcagccagc aaggtggctt
    13261 ccggtagcga actcactacc gcgatccccg catcttccag cgtggcgatt tgctgcgagc
    13321 ggcattgcgg gtcacgttca gtgcctgtca ccgtggcaat ggcatacagt ggttgattgt
    13381 ctgaacgcgc ggcacaggct ttttgccagg cgctcaccag cgaggcggca ggatcggcgg
    13441 tcgcaccgaa gccaatcacg acatcaagca gcaacacgcg cacttgcggt ttagcgccga
    13501 gatcggcaat taacaggttg cgtaaggtcg ggtcgatcat cggatgggga cgcccgacgg
    13561 tgtaaaaatc atcgcccagg tcgaggatct gatgtccgtc ggcgtccagc atcatgccat
    13621 gttgatgggt atcgtcggct tccacgccaa ggtgtccggc aagtaatccc gccgcttcag
    13681 cagccagcgt accgccggta tacaaaccgc aaataaatcc gctgctgaca ggcgctatcg
    13741 cgttacgtcg cgcagtgacg cgtgaaagca gacaagccag acgagcggcc tcatccagcg
    13801 aggaggcaaa ccagacattc tcgtcgcggg ccaccgccgg ggtataacct aaaaacagcg
    13861 ccaccgtcgg tttgccagtt gctttcatgg cattaacaat tttcagacgc acagtttcgg
    13921 caggtggttt tgaaacaaat gccagcactt cgcttttctc gtctgcactg agcatttcca
    13981 gcgccgttag cgcactgatg ccgcccactt cacggctgag atcgcgcccg ccaagaccaa
    14041 tcgcgtgagt aattccctcc cctgccagcg caatctgtga gcacagctcc tgaatcccgg
    14101 taccggaagc gcctatgacg ccaatgttgc cttccggcat cacgttagca aaagccagcg
    14161 gcgtggcggc aatcatcgac gtaccgcagt ctggccccat caccagcaag cctttttccc
    14221 gcgcgcgggt tttaagttgg atttcatctt ccagcgtgac gttatcggag aacatcatca
    14281 cgttgaggtt gcgatccagc gcctggttcg ccagctccgc cgcatactca ccagccactg
    14341 aaatcagcgc cagattggca tcgggtaatt tctgactggc actgtcccag cgacgcacct
    14401 gcgtcaacgc ctggctgctg cctgaaccct gcgccagttg ttttagcgcc tcttcaagct
    14461 gctgcataac cgcctgcgcg atccccgcat ccgccgcttc gctacgaatt gccacgcaaa
    14521 tatcgttcgg cgtggcgtta ttaaaatcgt catgccagaa acctgtagta tctaataacg
    14581 ctttattggc gggcgtaccc atcattacgg aaacatcatc aacattttct gattcgctga
    14641 gttttcgtga aataatcatt aaactgaccg aatcctgaaa acaccctttt ttaataaagg
    14701 cgtggatcat aacaactcct taaaaattgc acccatggca attgcacgag cagtgttaat
    14761 gtggcgatta cggtatcgat gacaacgagt aaataaggtg atgaatatca cacgtggatt
    14821 attaacaaaa gaattcaaat aaccgccaga ctattacgcg ttatttttta ataagcatgg
    14881 ttgttttaaa aaccaggtta aaagcacgaa gattatcaaa taaattctaa ctatattttt
    14941 ttgcctgtct ggatcacata atccagatat tttccctgta tgttaattgc agtcatgctt
    15001 cacaccgtcg ttaaaaagga agacagatga aaatcagtcg ggaaacactc caccagctaa
    15061 tcgagaataa actctgccag gctgggttaa aacgtgagca cgctgcaacc gtggctgaag
    15121 tattggttta cgccgatgcc agagggatcc actctcatgg cgcggtgcgc gttgaatact
    15181 acgccgaacg catttcaaaa ggcggcacca accgcgaacc ggagtttcgt cttgaggaaa
    15241 ccggaccgtg ctcggcaatt ttacatgccg acaatgccgc cggacaggtc gcggcgaaaa
    15301 tgggtatgga acatgccatc aaaaccgccc agcaaaaagg cgttgcggtg gtcggtatca
    15361 gccggatggg tcacagcggc gcaatctctt attttgtgca gcaggctgcc cgcgccggat
    15421 tgattggcat ttcgctgtgc cagtccgatc caatggtggt gccgtttggc ggcgcggaaa
    15481 tttactacgg cactaaccca ctggcctttg ccgcgccggg agaaggcgat gagatcctta
    15541 ccttcgatat ggcgactacc gtacaggcat ggggaaaagt gctcgacgcc cgctcgcgca
    15601 atatgtctat cccggatacc tgggcagtcg ataaaaacgg tgcaccaaca accgatccgt
    15661 tcgcggtaca tgctctgctc cccgccgctg ggccgaaagg gtatggcctg atgatgatga
    15721 ttgacgtcct ctcaggcgtc ttactcggct taccgttcgg gcgacaggtt agttcgatgt
    15781 atgacgattt acacgccggg cgtaatttgg ggcaattaca tatagttatt aacccgaact
    15841 ttttctcctc cagcgaatta ttccgtcaac atcttagcca gaccatgcgc gaattaaata
    15901 ccattacccc cgcgcccggt tttaatcagg tttattatcc cggacaggat caggatatta
    15961 aacaacgaaa agccgccgtc gaaggcatcg aaattgttga tgatatttac cagtatttaa
    16021 tttccgacgc gctttataac acgtcatacg aaacgaaaaa tccctttgcg caataattat
    16081 tgagacagga ctttcttatg attacacatt tccgtcaagc tatagaagaa acgctgccct
    16141 ggctttcctc ttttggcgct gacccagcgg gtgggatgac ccgtttactt tattcgccgg
    16201 aatggctgga aacccagcag cagtttaaaa aaagaatggc agcaagcggg ctggaaacac
    16261 gtttcgatga agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag
    16321 tggttctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat
    16381 tcggtgcgct ggcggcgtgg ctggctattg actggctgaa aacgcaatac ggcgcgccgt
    16441 tacgtacggt cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgttt
    16501 tctggggcag taaaaatatt tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg
    16561 atgccaaagg aaatagtttt gtcgatgcga tgaaggcttg cggatttact ctgccgaacg
    16621 ccccactaac tccgcgtcag gatattaaag cctttgtcga actgcatatc gaacagggct
    16681 gtgtgctgga aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc
    16741 gttatacggt gacgctgaat ggcgaatcaa accatgcagg caccacgccg atgggttatc
    16801 gtcgtgatac ggtttacgct ttcagtcgca tttgccatca gtctgtcgaa aaagcgaaaa
    16861 agatgggcga cccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtga
    16921 atgtagtgcc gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctaccgtgc
    16981 tgcgcgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggata
    17041 ttggtattga tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagttgg
    17101 tcgccaccct gacagaattg tgtgaaagcg aaaaactgaa ttaccgggtg atgcacagtg
    17161 gtgccgggca cgacgcgcaa attttcgcgc ctcgcgtgcc gacctgcatg attttcattc
    17221 ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattacc gaccttgccg
    17281 aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtta
    17341 taaatgggat atttaaataa cgtcaccggt taccgcgatg atttactggc taaccgtgcg
    17401 attgtgaaac acggtaattt cgcactgtta acaccagacg gtctggtaaa aaatattatt
    17461 ccgggctttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt
    17521 gttgattatc tggtcacact gcatcaaaac ggcggcaacc aacagggctt cggtggcgaa
    17581 ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaagggaaa
    17641 acatttacct taagtgaagg tggctatctt tattgcccgc caggctcctt aatgacgttt
    17701 gttaacgccc aggccgaaga cagccaaatc tttttatata agcgtcgcta tattccggta
    17761 gaaggtcatg cgccgtggct ggtttctggc aatgccagcg aactggaacg cattcattat
    17821 gaaggcatgg acgatgttat tctgctggat tttctgccca aagagttagg ttttgatatg
    17881 aacatgcata tcctctcttt tgcaccaggt gccagccacg gttatatcga aacccacgtt
    17941 caggaacacg gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg
    18001 atcccggtga aaaaaggcga ttacatcttt atgggcgctt attctttaca ggctggttat
    18061 ggcgtagggc gtggtgaagc gttcagctat atttactcga aagattgcaa ccgcgacgta
    18121 gagatttaag tcataccact cgcctgatta tttttagcta tgttggattt tgccacggtt
    18181 tataccgtgg ctttttttgt agtcaattca ggtaaaaaaa gatgcgatat aaatcgcatc
    18241 cgctttaaag gtaatagtca gtttttaatt ccctgaccta ttttaatggc gcaggcaata
    18301 tttcgcgcac tgttgaagag attggtttca ccgctggcga gcacttcggc taaaggtgcc
    18361 agacgtggca aaatgctgaa taccgcatca atgccgtact ggtgcaccac ttccacgcca
    18421 tcacccaata cgccagcaat cccaatcacc gggacattaa actgcttcgc caccgacgcc
    18481 acacccagcg gcgctttacc gcctgccgtt tgcgagtcga tgcgcccttc cccggtgatc
    18541 accagtgctg caccctgcac cgcctgcgca agattgaccg cattcaacac aatctcaata
    18601 cccggtttaa tatccgcatt gaggaatacc gccgccgcaa tgcccatacc gcccgccgcg
    18661 ccgccacccg ccatctggca gacgttgatt tcagttagct gttgtaaaac acgggcgtaa
    18721 ttttgcagcc cctgttccag ctcttcgacc atctccggcg ttgccccttt ttgcgggcca
    18781 aacaccgccg ccgcgccgcg tgcccctacc agcgggttat caacgtcaca tgctacttca
    18841 atatgacagt tcgccagacg cggatcgcat tcattcatct caatacttgc cacgcgcgct
    18901 aaattgccgc cgttagccgc cagcggctga ccgtcggcat caaggaaacg cacaccgagc
    18961 gcctgcgcca tgcccatacc gccgtcgacc gtcgcactgc cgccaatgcc gagaataata
    19021 tggcgaatgc cattatccag cgcatggaga attaactccc ccgtaccaaa actggaggcc
    19081 agcaacggat tacgcttttc aggcgcgacc agcatcaggc cacttgctgc tgccatctca
    19141 atcaccgccg ttttcccgtc gccggtaatg ccataaaaag cattcacttt ttcgcccatc
    19201 ggcccgcaga cttcaagctt cacgatgtta ccgtccgtcg cggcgaccat cgcctccacc
    19261 gtcccttcgc cgccatccgc tatcggcaaa cagatatagt gcgcatcggg aaagagggtc
    19321 gaaaacccgg ctttaattgc ctgacaacat ttttctgcac ttaagctctc tttaaaagag
    19381 tctggcgcaa tgacaatctt cattatcaat cctttttcaa caaattattc gatgcgttcc
    19441 cacggaataa gattatccat aagaacagcc agtaaaattc cgaccagtaa gccgttactg
    19501 agcagaggac gaagcgttaa tggaaggtct tgcagataca caggcggtaa tgccattaaa
    19561 aatatgccga caaataacgg caatgcgagt cgataaatat tgcgagcggt aaacgttatt
    19621 tgctggctaa acaccagcgc ggaaaagagt aaaggcagat aagaaaccag catgactgca
    19681 ctactcacgg gtaaagggat actacaaaac agtcgcgtga gtgccggaac cagtgccacc
    19741 agcaggcaaa taacgctgcc ataaataaac gaacgccgcg tgtaatcgcc tgtctgggtt
    19801 aataaaccaa tggatgaaac aaacggtgaa aatggaatta ccgccagcgg cacggttatc
    19861 agtgtcataa atccggtcgc cacaaagctg cgacgataac gcgtattccc cgcgccctgc
    19921 tgcggataaa aaacatccgt gccccgaatc gcaccgtagg tattgctgat atttaccaga
    19981 cctgtaatca ccgccgtcag aataatcccc ggcgacaaag caccgccact gccgagcggg
    20041 aaccactgcc agtgcaactc accggagagc gaatgcgaag aaggaaagca aaagtaccac
    20101 aacaaccagc cggttattgt accgaccagc aggccgtaac gggcaaaacg ttgtggcagg
    20161 aaaataatca tcgccagtac caggcacatc accgccaccg agagcgcgaa cggcggtaat
    20221 tgaattttaa aattcgggtc ggctatgcca aacggcagcc cgagcatacc tttgaaaaag
    20281 atagtggtca gttgcgcacc cagcatcaac ataaacaaga ccatcacaga cggcgtaaat
    20341 aaccgtgcca ggcgatggcc taatccgcta aaaccaatca acatcgtcag cacgccggag
    20401 agcgcaatcc ccaccgccag actggtagcg atatcgttga tcggtgtccc gcgcgatgct
    20461 tcgccaaggg tgatagtcag gatggttccc caccacaggc cgcccggccc ctccataata
    20521 gcgcgacgat gaccgcagaa cgcctgagcg aagcaggcca gtgcggtagc aagaaaagca
    20581 tattgcgtga gcgtaagcag gctactttgc ggcaactgaa aagcagaaag tagcgtagga
    20641 ggaaccacaa ccgtgttgca aaaaataaag aaaaaccact gaaatcctga taacaggctt
    20701 tcgcggccca ctgcaaaatt gaacataaac acaccttaac cggagaatgc ccgccacaag
    20761 gacgggcatt gcagggccag attactgctg atgtttaagg ataaattgac ctttcggcgc
    20821 aacagggaag ccctgttcga tatcgtaaat cacatcacca cgtaagatgg ttttcgtgat
    20881 acgcgcgcca atagtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc
    20941 attggtaaga acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg
    21001 ggcgatacgg cctttttgct gcagaccgaa aatatctgct gcgttagtcg ccattaattt
    21061 gccgaacatt ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca tcacgtccat
    21121 gcagctttgc agaccagcga taccgcccca cgctttcatg atgttaccgg ctttcatttc
    21181 cggcgggcat ggagagtggt cggaaaccag gcagtctatt tcaccgttaa acagtttttc
    21241 ccacatgcct ttctggtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtacc
    21301 gatttcttcg aactgatcgg tatccagcac aaagtaatgc gggcaggatt cacaagtaac
    21361 atcctgacct tcctgacgtg cacgagtcac ttcctcaaca ccttccgggc tgctgacgtg
    21421 gcaaacgtgc agacggcaac cagcgacttt cgccaggtac agtacgcggc gaatcgcttc
    21481 cacttcggta aataccggac gcgaagccac atagtcatgg gcagttacgc gaccttcacg
    21541 cttcgcttct tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg
    21601 ctggcctagt tcacccagct tctgcgcgcc tttgaagaat tgccagtcat tgacgtcacg
    21661 gaagtcgttg tcgataccgc gatcgccaca ggtcgcaacg aagcatttga aaccgacaac
    21721 acccacttca tccaactcat gcagacggtc aatgttgtaa gacaccaggc caccgagttg
    21781 tgccgcatcg atagtcagct ttcctttagc ggcatcgaac ttcagttcaa ttgaggcgcg
    21841 gtcaaccgtt gcaggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt
    21901 tgctgctgcg cgagtaccgg tttcataacc ttcccagtgg ctgcgacccg gttcagaaat
    21961 atgggtgtgc gcatcaacca tgcccggcga aaccaccaga ccagacgcat ccataacgtc
    22021 ttttgcatcg cccagatcct ggccgatagc agcaattttt ccgtctttaa cggcgatatc
    22081 tacaacgcga gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga
    22141 cataacaaac tccttgtaaa atacagattg gtttttaact caatgttcaa tatcgtgatt
    22201 aattaaccta tggttttttg ttctcctgtt ttttctgctg ttgtacgttt ctttaataag
    22261 gcgtaggccg caaaggcgac gatgacgccg acaaaccatg aaacacgtga taacggttcc
    22321 ataaagggaa taaacttacc gccaagagat aaaataacgg ccaccagagt tactgaaaac
    22381 gcggtgaggt taaaaccgtt atcgtaatat ttataatcgc caggtgcggt gtacagttca
    22441 tcaagattaa tttgcccacg catcaccaca aaataatgcg ccatcatcac accaattacc
    22501 ggaccaagca ttccgccgat aatatcgagg aacagataaa tactgtcctg attttccatt
    22561 aatttccacg ggcagatcag caggctgata atactggcaa tcagtacgcc gtttttatag
    22621 gtcagtttcg tcggtgcaat ggcggcaatc tgataaccag ccggaataat attaccggtg
    22681 gcgttagtag agatagtcgt catcagaata accagtaccg caaagaacga ggcgaacagg
    22741 ctgtcccaac gctgaacaat atccagcacg ttccaggtgt cagcgccgta atgaatactg
    22801 gctccggcaa taatacatac cccggcgacc gcaaacagaa tataggccac aactaaaccc
    22861 agcgtttgcc ccagcgcctg ctcacgaaac gagtgggcgt tttgcgtaaa gtcggatgcg
    22921 ctcaccgccg gtgccgccca gaccgcaacc accgcgttta tcaccaccag aaacaggaag
    22981 ccactgtttt ctgctttctg aataccgccc ggaatgtagt caaagattgg accgatcccg
    23041 accagcgaaa tcgcccaaat cgccataccg ccgaaaacga tatagatgca cgggttaaga
    23101 atggcagtga atttatttaa aacttttccg ccgccaaaac caataccaac gttaaccagc
    23161 cagaagagta aaaaagtaat taagcccggt agagaaaggc ctaacagcgt gaaatcgcca
    23221 ccgagagtca aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc cagtgacccc
    23281 gcgtaacatt gcaggccaaa ccacataatg gcagcaatac cgcctcttaa taatccggga
    23341 aacagtgcac cacgtacgcc gtaagaagca cgcaggatca tggcaaacgg tacgccgtat
    23401 ttactgcccg cagcaccgtt taataccatt accgcggcaa tgaaaaaggc gctgaggata
    23461 attgccagca taatactaaa ggtagacaag ccgagaataa aaaagccgcc aaccatcaca
    23521 taattgggaa cgttatgaac cgaacccatc cataaggtaa aatagttaaa tgttttccag
    23581 gtccgctgac tttgcgtttt cggcagtaga tcttcgctat agccgcgttg ctggaataat
    23641 tttctctgat gttccataaa tccctcttaa tttcacgtat
//
LOCUS       DADTFG010000074        23385 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.074, whole genome shotgun sequence.
ACCESSION   DADTFG010000074 DADTFG010000000
VERSION     DADTFG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.074"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(375..2045)
                     /gene="betA"
                     /locus_tag="J1138_002659"
     CDS             complement(375..2045)
                     /gene="betA"
                     /locus_tag="J1138_002659"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HBA8454433.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIFFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2059..3531)
                     /gene="betB"
                     /locus_tag="J1138_002660"
     CDS             complement(2059..3531)
                     /gene="betB"
                     /locus_tag="J1138_002660"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HBA8454434.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGHTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGCQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANNTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3545..4132)
                     /gene="betI"
                     /locus_tag="J1138_002661"
     CDS             complement(3545..4132)
                     /gene="betI"
                     /locus_tag="J1138_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HBA8454435.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLSTD"
     gene            4261..6294
                     /gene="betT"
                     /locus_tag="J1138_002662"
     CDS             4261..6294
                     /gene="betT"
                     /locus_tag="J1138_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HBA8454436.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMINGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            7172..8260
                     /locus_tag="J1138_002663"
     CDS             7172..8260
                     /locus_tag="J1138_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA8454437.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(8302..9234)
                     /locus_tag="J1138_002664"
     CDS             complement(8302..9234)
                     /locus_tag="J1138_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8454438.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     LLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9326..9823)
                     /locus_tag="J1138_002665"
     CDS             complement(9326..9823)
                     /locus_tag="J1138_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBA8454439.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLAPVSGISGVWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10081..10686
                     /locus_tag="J1138_002666"
     CDS             10081..10686
                     /locus_tag="J1138_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBA8454440.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGANINKQDLTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            10726..11589
                     /locus_tag="J1138_002667"
     CDS             10726..11589
                     /locus_tag="J1138_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA8454441.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIVLPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDDQGITIGQHHHIEMSHCQRWLSPTMQMTAL
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     AVAVASMMGLGIGLTPSADDYLTGLVLILFIPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRSRENIHHFIHDIIYGVPRNSTQVIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            11579..13126
                     /gene="fdrA"
                     /locus_tag="J1138_002668"
     CDS             11579..13126
                     /gene="fdrA"
                     /locus_tag="J1138_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA8454442.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            13126..14544
                     /locus_tag="J1138_002669"
     CDS             13126..14544
                     /locus_tag="J1138_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA8454443.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIASANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKILGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            14570..14749
                     /locus_tag="J1138_002670"
     CDS             14570..14749
                     /locus_tag="J1138_002670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454444.1"
                     /translation="MNVLSGLRVAQESVGLISVAHQALVTTAGCGVNALSGLRMARES
                     VGLISVAHQAVWRWS"
     gene            14781..15731
                     /locus_tag="J1138_002671"
     CDS             14781..15731
                     /locus_tag="J1138_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBA8454445.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPIKPIGEFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            15741..17123
                     /locus_tag="J1138_002672"
     CDS             15741..17123
                     /locus_tag="J1138_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBA8454446.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGPSVPLAHAGGAGTRNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFKCEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGMAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMVAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGEHVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            17500..18550
                     /gene="yahK"
                     /locus_tag="J1138_002673"
                     /pseudo
     CDS             17500..18550
                     /gene="yahK"
                     /locus_tag="J1138_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
     gene            19172..19415
                     /locus_tag="J1138_002674"
                     /pseudo
     CDS             19172..19415
                     /locus_tag="J1138_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19432..20103)
                     /locus_tag="J1138_002675"
     CDS             complement(19432..20103)
                     /locus_tag="J1138_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBA8454447.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMHRLSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLHAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            20250..20525
                     /locus_tag="J1138_002676"
     CDS             20250..20525
                     /locus_tag="J1138_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA8454448.1"
                     /translation="MKIISKMLVGALVFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(20626..22212)
                     /gene="prpR"
                     /locus_tag="J1138_002677"
     CDS             complement(20626..22212)
                     /gene="prpR"
                     /locus_tag="J1138_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HBA8454449.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLASERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQIVLLLYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPVPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            22451..23341
                     /gene="prpB"
                     /locus_tag="J1138_002678"
     CDS             22451..23341
                     /gene="prpB"
                     /locus_tag="J1138_002678"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HBA8454450.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAEAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
BASE COUNT         5707 a         5884 c         6197 g         5597 t
ORIGIN      
        1 agtaaaaata aatcactact aataaattat acgaatcgtg tttaaagctc catgtggttt
       61 tggtctgact tatttttgta ctcgttacaa gaaccctttt cgtatatgct aaccgtgagc
      121 gggaatgaac aggtaaccat agccaggaac attagcggcg taattcttaa gctgagtggc
      181 atttcacctg cggtttatca gtgcattcat agcaccttca tcagccagat tgagcaagtc
      241 agcttagaat ttgctggcga gattgtcacg atgaagcatg agagttacct cagtgtttta
      301 tataaggatt cggtcctcat agtagaacgg taactctcaa tctgatcggt tcctgagtca
      361 gttcacatca cgactcattt tttcgctctc accggcatcc catttgccac aaaatatccc
      421 gccgtgctcc tcggcaacgc ttccttccca cgaatcatat ccgctatttt ctcgccaatc
      481 ataatggtcg tggcgttcaa attgccggtg ataatttgcg gcataattga cgcatccacc
      541 acgcgcaggc cttccagccc gtgtacgcgg ccttcgccgt caaccacagc catctcgtca
      601 taacccattt tgcaggtacc gcacggatga aacgcggttt cggcgtggtt acgcacgaac
      661 tcatcgagct gctcatccgt ctggcattcg acaccagggc tgatttcgcg gccacggtat
      721 tgatccagcg cgggttgatg catgatctcg cgggtgatgc gaattgcgtc gcggaactcc
      781 tgccagtcct gctcgtgcga catgtagtta aacagaattc ccggatgctg gtgcgggtcg
      841 cgggatttaa tccgcacatg cccacggctt ggcgagcgca tcgatccgac atggcattgg
      901 aagccgtgct ctttcactgc gttcgagccg ttatagttaa tcgccactgg caggaaatgg
      961 tactgaatat tcggccacgc aaattcctca cggctgcgaa taaatccgcc tgcttcaaag
     1021 tggttgctgg caccaacgcc ggtgccgcca aacagccact ccgcaccgat tctcggctga
     1081 ttccaccact gcagggcagg gtagagggaa accggttctt tgcactcgta ttgcagatac
     1141 atctccaggt gatcctgaag attttcgccg acgccaggta attcatgcac cagcggaata
     1201 tcaaactccg ccagcagttc agcgttgccg acgccggagc gttgcaggat ctgcggtgaa
     1261 gcaatcgcgc ctgcacataa cagcacttct ttattcgccg ccgcgcgggt cggaatggtg
     1321 ctgtcgcctt ccagccactc gacgcccacc gcgcgtttgc cgtcaaaaaa gatgtgatcg
     1381 gtcatggcgt gagtacgaat ggtcaggtta gggcgcgatt tggcctgatc gagatagcca
     1441 cgcgcggtgc tggcgcgacg gccatgcggc gtgacggtgc gatccatcgg gccaaaacct
     1501 tcctgctgat agccgttgag atcgtccgtg cgcgggtagc ccgcctgcac gcccgcttca
     1561 atcatcgctt caaacagcgg attgacgccg ggtttggagg tggtgacgct caccgggcca
     1621 tcgccaccgt ggtaatcgtt ctcgcccaca tcgcgagtct cggccttgcg atagtagggc
     1681 agacagtcga ggtagctcca gttctccaga ccgggttctt tcgcccagtt atcgagatcc
     1741 agcgcgttac cacggatgta acacatgccg ttgatcagtg atgacccgcc aagcccctta
     1801 ccacgtccgc actccatgcg acggttgttc ataaacggtt ccggttccgt ctcataggcc
     1861 cagttatagc gtttgccctg tagcgggaac gccagcgcgg cgggcatctg ggtgcggaag
     1921 tcaaagcgat agtccgggcc gcctgcttca agcagcagca cggttgtgtt cggatcttca
     1981 gtcagacggg tagcgagaac gttgccggct gagccggcac caataatgat gtagtcaaat
     2041 tgcaaataaa cctcctggtt agaatatgga ctggaattta gccatctcaa cctggatgga
     2101 cttcacctgg gtgtaactct ggagcgtcat cacgccgttc tcgcgaccaa tgccggagtg
     2161 tttgtagccg ccaacgggca tctcagcagg ggattcgccc caggtgttga tccagcaaat
     2221 acccgcttcc agctgatgaa tgacgcgatg cgcgcggttc aggtccgccg tcacgatacc
     2281 cgccgccaga ccgtagtcgg tattgttagc gcggcgaatg acttcgtctt ccgactcgta
     2341 ggtcagaatg gacatcaccg gcccgaaaat ctcttctcgc acaatggtca tttcgtcgcg
     2401 gcaatcggtg aacacggtcg gtgcaaccca tgcgccgtta tcgaagccat cacctttcag
     2461 tacatcgccg ccgcacagta cgcgcgcgcc ttcctctttg cctttggcga tgtaacgcag
     2521 cacgttatcg cgatgcggga agctgaccag cgggccaaag ttagtttgcg gatcgaaaac
     2581 gtcgcccgcg cgaatgcgct caacgcgcgc cagaaccttt tgttcaaatg cggctttgca
     2641 tttcgtcgga acgaagacgc gagtgccgtt ggtacacacc tgaccggagc tgaagaaatt
     2701 cgccatcatg gcgatatcgg cggcgagatc gagatccgca tcgtcaaaaa cgatcagcgg
     2761 cgatttaccg cccagctcca tggtcacttc tttcagggag gaagctgccg agttagccat
     2821 cacttttttg ccgctggcga cgccgccggt aaatgacact ttggcaatgc ccggatgatc
     2881 ggtcagatat tgcccggtct ccgcgcccac gcccggcaac acgttaaata caccgtccgg
     2941 caggcccgct tcgctgtaaa tttcagccag ctttaacgcg gtaagcgggg tgacttcgct
     3001 cggtttgaag atcatcgcgt tacccgccgc cagcgcgggg gcggatttcc acaaagcaat
     3061 ctggatcggg tagttccatg cgccaatccc cgccaccaca cccagcggtt cgcggcgggt
     3121 ataaacaaat gacgtctcac gcaacgggat ctggcagcct tccagcgccg ggatcagccc
     3181 ggcatagtac tccagcacgt ccgcaccggt aacgatatcg acggttgagg tttccgaata
     3241 tgcttttccg gtgtcgaggg tttccagttt tgcgagttcg tcattgcgtt cacgcagaat
     3301 atcaacggcc cgacgcagaa tacgcgagcg ttccatagcg gtcatcgccg cccagatttt
     3361 ttgcccttgt tgggcactct tcacggcgcg atcgacatcc tcacgcccgg cggcctgcac
     3421 ggtcgccagc acgttaccgt tggccgggtt aatggtctcg aaggtgtgac cgctggtggc
     3481 ggaggtataa ccaccatgta tataaagctg ctgttctgcc attcgggaca tcacgtctcc
     3541 tcggttaatc ggtggataaa tgctgagtaa taaagtgacg ggtcagcgag tgtgcgagag
     3601 gtttatccag cggtttgccg ctcagtgccg cgcgcagcca taatccgtca attagcgcgg
     3661 caaggccgta gcccgcttcc tgtgcctgtt ggcgcggtaa ctcgcggcga aattcgctca
     3721 ccagattcga caataagcgg cggctactga cctgctgcaa gcgatagagc atcggctgat
     3781 gcatactgct ggcccagaag gccagccagg ctttcatcgc cgcactgctc acctgggttt
     3841 catcgaagtt tccaccaaca atcgcctgta atcgcagctc tgcactgccc tgcggaagtg
     3901 catgtaatcg attcaaaacc gcgtcacgca gctgactggt gatatcgcgc atggttgctt
     3961 ccagcagacc atttttgtcc ctgaaatagt ggctgatgat ccccgtagaa acgcctgcac
     4021 ggcgggcgat ctgcgcgatc gttgcatcgt gcatgcccac ttcatttatt gcttccagcg
     4081 tggcgtcgat cagttgtctg cgccggatcg actgcatccc caatttgggc attttcgcca
     4141 ctccattcat cagcgttgtt tatctattaa agcggttatt gattggacgt tcaatataaa
     4201 atgtgtctta attgttacta atttgatttt aaatggtaac aataacagtg gggatactgg
     4261 atgacagacc tttcacacag cagggaaaag gacaaaatca atccggtggt gttttacact
     4321 tccgccggac tgattttgtt gttttccctg acaacgatcc tgtttcgcga cttctcggcc
     4381 ctgtggattg gccgcacgct ggactgggtc tctaaaacct tcggttggta ctatctgctg
     4441 gcggcaacgc tctatattgt ctttgtagtc tgtatcgctt gttcgcgttt tggttcggtg
     4501 aagctcgggc cagaacaatc aaaaccggaa ttcagtctgc tgagttgggc ggcgatgctg
     4561 tttgctgccg ggatcggtat cgacctgatg ttcttctccg tagccgaacc ggtaacgcag
     4621 tatatgcagc cgccggaagg cgcgggacag acgattgagg ccgcgcgtca ggcgatggtc
     4681 tggacgctgt ttcactacgg cttaaccggc tggtcgatgt atgcgctgat gggcatggcg
     4741 ctcggatact ttagctatcg ttataatttg ccgctcacta tccgctcggc gctgtacccg
     4801 atcttcggta aacggattaa cgggccgata ggtcactcag tggatattgc tgcggtgatc
     4861 ggcaccatct tcggtattgc cactacgctc ggtatcggtg tggtacagct taactatggc
     4921 ttgagcgtgc tgtttgatat tcccgattcg atggcggcga aagcggcact gatcgccttg
     4981 tcggtgatca tcgccactat ctcggtgaca tccggtgtcg ataagggcat tcgcgtgtta
     5041 tcggaactca acgttgcgct ggcgctggga ttgatcctgt tcgtgttgtt tatgggcgac
     5101 acctcgttcc tgcttaatgc actggtgctg aatgttggcg actatgtgaa tcgctttatg
     5161 ggcatgacgc tcaacagttt tgccttcgac cgtccggttg agtggatgaa taactggacg
     5221 ctcttcttct gggcatggtg ggtggcatgg tcgccgtttg tcggcttgtt cctggcgcgt
     5281 atctcgcgtg ggcgtaccat tcgccagttc gtgctgggca cgttgattat tccgtttacc
     5341 ttcacgctgt tatggctctc ggtattcggc aatagcgcgc tgtatgaaat catccacggc
     5401 ggcgcggcat ttgccgagga agcgatggtc catccggagc gcggcttcta cagcctgctg
     5461 gcgcagtatc cggcgtttac ctttagcgcc tccgtcgcca ccattactgg cctgctgttt
     5521 tatgtgacct cggcggactc cggtgcgttg gtgctgggga atttcacctc gcagcttaaa
     5581 gatatcaaca gcgacgcccc cggctggctg cgcgtcttct ggtcggtggc gattggcctg
     5641 ctgacgctag gcatgctgat gatcaacggg atatccgcgc tgcaaaacac cacggtgatt
     5701 atgggcctgc cgttcagctt tgtgatcttc ttcgtgatgg cggggttgta taaatctctg
     5761 aaggtagaag attaccgccg tgaaagtgcc aaccgcgata ccgcaccgcg accgctgggg
     5821 cttcaggatc gcctgagctg gaaaaaacgt ctctcgcgcc tgatgaatta tccgggcacg
     5881 cgttacacta aacagatgat ggagacggtc tgttatccgg caatggaaga agtggcgcag
     5941 gaactgcggt tgcgcggcgc gtacgtggag ctaaaaaacc tgccgccgga agagggacaa
     6001 cagttggggc atctggattt gttggtgcat atgggcgaag agcagaactt tgtctatcag
     6061 atttggccgc agcaatattc agtgccgggc tttacctacc gcgcacgtag cgggaaatcg
     6121 acctactacc ggctggaaac cttcctgtta gaaggcagcc agggcaacga cctgatggac
     6181 tacagcaaag agcaggtgat caccgatatt ctcgaccagt acgagcggca ccttaacttt
     6241 atccatctcc atcgtgaagc gccgggccat agcgtgatgt tcccggacgc gtgattgtta
     6301 ttgcataaaa ccgcgccatg tctgcatatg gcgcggtttc tcttacctct tgatacgtta
     6361 tatctgtgta cagttaagcc cttagtatcg attgatgatt gccaggcaac agataataaa
     6421 aaagaaaaga actattgaag cccaaaacct acgtttggac tgttgtgaat gttcaataag
     6481 cttagtctta tttaatgtaa atattggtgc taatttgaaa tgacgctttc ttcatgggga
     6541 acgcgtagcg caaatcaact aaaatcaatg taaccgtttg ttgtttaagg tttaattgtc
     6601 tgtgtctgat attttattga ttgttttaaa tattgttttt attgtcattg ttataatgtt
     6661 ggctatctat tgctgaatgg attcagtctt aaaaagcagg ttgttaaggg ataggcatag
     6721 agtaatgacg tgtatgcata accaacatct ttactcatta tgtcattgaa tgttgacgct
     6781 atgtgtttat gaggttgagg tattttcagt tgatctggat tgttaaattc atataatgcg
     6841 cctttactca tgaatgggcg tcaatacgta gtgggaaatt ataaatatcg aaatagtcca
     6901 actacttctt tattaccaaa aatgagtatc tgaattttaa tattgcattc ttgcgtgatt
     6961 atctcctgag tttgacttgt gattaccttt ttaaggtatt tagcgtaact gtttttgagc
     7021 gagcatcaga ggtaaagata atcttcttga tagtgatgtg ggatgttata cgtatggcat
     7081 cgctaatgtt tatggttacc ccttatgtgt gctcaggaat ccacaggtaa tcactcatac
     7141 tgaacagcga taaaagataa aaggtgtgtt catgaattca tgtgattttc gtgtttttct
     7201 gcaagagttc ggtacaacgg ttcatttgtc attgcctggt agcgtatccg agaaagaacg
     7261 actgctactc aagctgctga tgcagggaat gtctgtaaca gaaatatcac agtacagaaa
     7321 tcgcagtgca aagaccattt cacatcaaaa gaaacagctc tttgagaaac tggggattca
     7381 gagcgatatt actttctggc gggatatttt ctttcagtac aatccggaga tcatatccgc
     7441 cacgggtaat aatagtcaca aatatattaa tgataatcac tatcaccata tcgtcacgcc
     7501 tgaagccatc agtctggcgt tggaaaacca cgagtttaaa ccgtggatcc aaccggtttt
     7561 ctgcgcgcag actggagtac tgacgggctg tgaagtgctt gtccgctggg aacatccaca
     7621 aacgggaatg atcccaccgg atcagtttat tcctctggcg gagtcatccg gtcttattgt
     7681 cataatgact cgccagctga tgaaacagac tgcggatatt ctgatgccgg taaaacattt
     7741 gctgccggac aatttccata ttggtatcaa cgtctcggcg ggctgttttt tggctgcggg
     7801 atttgaaaaa gagtgtctga acctggttaa gaaattaggt aacgataaaa tcaagctggt
     7861 tcttgagctg acggaacgta atcctattcc ggtaacgcca gaagccagag cgatatttga
     7921 cagccttcat cagcacaata ttacctttgc gctggatgac tttggtacgg gttatgcgac
     7981 ctatcgttac ttgcaggcgt tcccggtcga ttttattaaa attgacaagt cgtttgtgca
     8041 aatggcgagt gtcgatgaaa tctccggtca tattgtggac aatattgtcg aactggcgcg
     8101 taagcctggt ctgagtatcg tggcggaagg ggtagaaacc caggagcagg cggatttaat
     8161 gatcggtaaa ggcgttcact ttttacaggg ctatttgtac tctccgccag taccgggtaa
     8221 taaatttatc tctgaatggg taatgaaagc aggtggttga tgtaaaccgc tattcacagc
     8281 gcatcgggag gttggcagcg attaattctc ccgatgcagt aaatcctgat agatccccgt
     8341 taatatgcca tttggcgcaa attgtttttt aatccactgc gtcacctgac cggtggcaga
     8401 gtgctgggtt gcgagtaaca tgcgtgaatc ctggcgcggg ttatggattt gtcgggtaac
     8461 cagcaaggat tgcgccatcg cctcacggac catgtaatcc ggcaaaaaac caattccttc
     8521 acctaaaatc tggcactgac atttggtgtt gaaatctggc accagaattg actcctgccc
     8581 atgcagcaac cagcccactt ttttattaat cgtatgcgcg gtgtcctcca ccatgatatt
     8641 agggtagaga cgcagctggc tttcggcgat cggctccggc acaaaggcta atgggtgatc
     8701 cggggcgatg gcaaaagccc agcgaatcgc gccaatctcc gtgtaatcaa tgccgccacc
     8761 gtccagcagc gtgtcgggtg cgccgatagc gatatttgcc tgattattaa taatcgcatc
     8821 ccagacgccg ttatacactt cggtcgtcac ggtaatctgg caggtaggaa actgcttttt
     8881 cagcacctgt aataagcgcg cggtgtgttt gggcgtataa agcagcagat taatacaaat
     8941 gcgcacacgc gcttctatgc cctgcgaaat agtatcgata ctgcgtttaa tggcatgaaa
     9001 atcattcagc aggtcggtag ctttgcggaa aaaataacgc ccggactccg ttaactcaat
     9061 gctgcgggta ctgcgagtga acagcaccac atccagcccc gtctccatac gtttaatggt
     9121 gtagctaatg gcggaagtgg ttaaacccaa ctcttctgcg gctttactga agctgccaaa
     9181 acgtgcagcg gtagtaaatg ccaacagatt ctcttcggta aaaattgagt tcattaaacc
     9241 attcccgcca ttatctaata tgaatttctc cagccatgac tgtagaggta tcggttaaag
     9301 atagataatc atttttgaat aacttttaat acccgtcgcg tttagtcgtc gcttcgctta
     9361 atttttgtcc gccgtattct gaaattatcc ccacgcagtt acccgccaga aaacccagta
     9421 atgtcaccgt ccagttcatc ccgctggcaa aaaatagcgt catgccgaga aagcctccgg
     9481 gaataaaaga cagtaaccag aaacgccctt gccagaccac caccgccgag aagggtacag
     9541 tcgtaatcac gctcgcccag accccggaaa tcccactcac cggggccagc caaccggcag
     9601 ccagcgcggc gaaaaacgcc cacaccatcc cggaataatt aaccgccagg cttctgataa
     9661 accccgattt accgccgcct gcggcataaa aactgcaaaa ggcgacgaag ccaatggttc
     9721 ctaataattc ccatccctga gagagcccga catgtgaaga cactaactgc caaagccctg
     9781 cacaaatacc gacggtgaca cccgttgccg tgagtccgtt catcttgttc ccctctggtt
     9841 agacatggac gaaagctact cttacatgtg cagactatag agaaataaaa cctgattgag
     9901 tgaaagggta tcgggtcaaa gaaacaaata ttgcactacc gcacactgcg aaaagattgt
     9961 tgaacatcat tcaacaaaaa ggcgaaatac ggcgaattaa atttaagcgg gttcttttat
    10021 cagattatcg ttgctattct caattcatcg aaaacagcca caaaaaccag gtgaatgtga
    10081 atgactataa aaaatctacc tgcagactat ttattggctg cgcaacaggg ggatatcgat
    10141 aaagtaaaaa cctgccttgc gctgggtgtc gatattaata cctgcgatcg tcaggggaaa
    10201 acggcaatta cgctggcaag tttatatcag caatatgctt gcgttcaggc attaattgac
    10261 gccggagcga atattaataa acaagacctg acatgtttaa atcctttttt aattagctgt
    10321 ctgaacgatg atttaacgct actacgaatt attttaccgg ctaaacccga tcttaattgc
    10381 gtaacccgtt ttggcggtgt cggcctgacg cctgcctgtg aaaaaggcca tttaagtatt
    10441 gtaaaagagc ttttggcaaa tacggagatt aacgttaacc agactaacca tgttggctgg
    10501 acaccgttgc tggaagcgat tgtgcttaac gatggcggga ttaaacagca ggcgattgtc
    10561 cagttattgc tggaacacgg tgccagcccg catctgaccg ataaatatgg caaaacgccg
    10621 ctggaactgg cgcgggaacg gggctttgaa gagattgcgc agttgctgat cgccgcaggt
    10681 gcataaaccg ggaggcttgt tatcaacaca ccagaaaaac ggtgtgtgtg ggcgctaact
    10741 gcggatgctg attttctggc gcagcggggg caaggacagg ttgaacaggt ctttgccaga
    10801 gcggtaaata tcgtactccc ggctcgccag cagttgctga cgctgctttg tgaagagtac
    10861 gacaatgcgc ctaacagttg tcggttggca ctcactcact ttaatggtct gttccggcat
    10921 ggtgataagg ttcagtttga cgatcaaggc attacgattg gtcaacatca tcatatagag
    10981 atgagtcatt gccagcgttg gctgtcccca accatgcaaa tgaccgctct gaattttcac
    11041 cttatcgcct ggcaacagtg gcacgacatt attcatcagc acctggggga aaatgaaacc
    11101 ctgtttaatt atcgcggcga taatccgttt tatcaggcgt taaataaaga actacatatt
    11161 aaacgtcggg cagttattca ggccgtaaac gataaacaaa atatcgccgt agcggtcgcc
    11221 agtatgatgg gcttagggat tggccttacg ccatcagccg atgattatct gaccgggctg
    11281 gtacttattt tatttattcc cggacatccg gcggaaaaat acaaagagga attttatctc
    11341 ggtctgcaac gcggcagaaa taataccacg ttattaagtg ccataacgct ggaagccgca
    11401 ttacaacaac gcagccggga aaatattcat cattttattc acgatattat ttatggtgtc
    11461 cctaggaatt caacccaggt aatagaaaaa attaaacata ttggctccag ttctggctgc
    11521 gacatgctgt atggcatggc cgatggttgt gcgctgagcc aaacctacgg agggaattat
    11581 gtcagttaaa atagtcatta aaccgaatac ctattttgat tctgtctcgc tgatgtctat
    11641 ctccacgcgt gcaaataaac tcgacggcgt cgagcaggca tttgtggcga tggcgaccga
    11701 aatgaacaaa ggcgtgttga agaatttagg actgctgacg ccggagctgg agcaagcgaa
    11761 aaacggcgac ctgatgattg tcatcaatgg taaatcgggt gtggacaacg agcagttgct
    11821 ggcggagatt gaagaactgt tcaacaccaa agcgcaaagc ggctcgcacg aggcgcgtta
    11881 cgccactatt gccagcgcca aaaagcatat cccggaaagt aacctggcag tgatttcggt
    11941 caacggtctg tttgccgctc gcgaagcgcg tcaggcgctg caaaatgacc tcaacgtgat
    12001 gctgttttcc gataatgtct cagttgaaga tgaactggcg ctcaaacagt tggcccatga
    12061 aaaagggctg ctgatgatgg ggccagactg cggcacggcg attatcaacg gcgcggcgct
    12121 ctgctttggt aacgccgtgc gtcgcggcaa catcggtatt gttggcgcat ctggcaccgg
    12181 cagccaggaa ttgagcgtcc gtatccatga atttggtggc ggcgtttcac aactgatcgg
    12241 caccggcggg cgcgacctga gcgagaaaat cggtggcctg atgatgctcg atgccatcgg
    12301 gatgctggaa aacgatccgc aaactgaaat cattgtgctt atctccaaac cgcctgcgcc
    12361 tgcggtggcc cgcaaagtgc tggaacgcgc acgcgcctgc cgcaagccag tggtggcctg
    12421 cttcctcggt cgtggcgaaa cgccagtgga tgagcagggg ctacagtttg ctcgcggcag
    12481 caaagaggcg gcattgaaag cggtgatgct ctccggcgtg aaacaagaac atctcgacct
    12541 gcatacgctt aaccagccgt tgattgcgga tgtgcgtgcg cgtctgcaac cgcagcagaa
    12601 atacattcgt ggtctgttct gcggcggtac gctgtgtgac gaaaccctgt tcgcggtgat
    12661 ggaaaaacat ggcgatgtct acagcaatat ccagcccgat cctgaattcc gcctgcaaga
    12721 tatcaaccgc agcatcaaac acaccttcct cgactttggc gatgatgact tcaccaacgg
    12781 caagccgcat ccaatgattg acccgaccaa ccgtatcagc cgcttgatcg aagaagcgcg
    12841 cgatccagaa gtggcggtga tcgtgatgga ttttgtgctc ggatttggat cgcatgaaga
    12901 tccggtcggt tccaccatcg aggcgatcaa agaagcgaaa gcgatcgccg ctgccgaagg
    12961 acgcgagttg atcattctcg cctatgtgct gggcaccgat cttgatacgc catcattaga
    13021 acaacaaagc cagatgctgc ttgatgccgg agtgattctg gcgagcagca gcaccaatac
    13081 cggattgctg gcgcgtgaat ttatctgcaa aggggaggaa gcctgatgag cccgtcactg
    13141 tttagccaac cgttgaacgt tattaacgtc ggtatcgcca tgtttagcga tgatctgaaa
    13201 aagcagcatg tagaagtgac ccaactcgac tggacgccgc cggggcaggg caatatgcag
    13261 gtggtgcagg cactggataa tattgccgat tcgccactgg cggacaaaat cgcctccgcg
    13321 aatcagcagg cgctggagcg cattatccag tcgcatccgg tgctaatcgg ctttgatcag
    13381 gcgattaacg tggtgccggg catgacggcg aaaaccattc ttcacgccgg gccgccggtc
    13441 acctgggaaa aaatgtgcgg cgcgatgaaa ggcgcggtca ccggagcgct ggtgtttgaa
    13501 ggactggcga aagatctcga cgaggcggct gagctggcgg cttccgggga gatcaccttc
    13561 tcgccgtgcc acgagcacga ctgtgtggga tcgatggcgg gcgtcacctc ggcctcgatg
    13621 tttatgcaca tcgtgaaaaa caaaacctat ggcaacatcg cttataccaa catgagcgag
    13681 cagatggcga agattttgcg tatgggcgct aacgaccaga gcgtgatcga ccgcctcaac
    13741 tggatgcgcg atgtgcaggg acccatgctg cgcgacgcga tgaagattct cggcgaaatc
    13801 gatctgcgct taatgctggc gcaggcgctg catatgggcg atgaatgcca taaccgcaat
    13861 aacgccggga cgacgctgct gattcaggcg ctgacgccgg ggattattca ggcaggttat
    13921 tccgtcgagc aacagcgcga agtgtttgag tttgtcgcca gcagcgacta cttctccggc
    13981 ccgacgtgga tggcgatgtg taaagcggcg atggatgcgg cgcacggcat cgaatacagc
    14041 accgtggtga ccactatggc gcgtaacggc gtcgagttcg gcctgcgggt cagcggcctg
    14101 ccggggcagt ggtttaccgg cccggcgcag caggtcatcg gcccgatgtt tgccggttat
    14161 aagccggaag attcagggct ggatattggc gacagcgcca tcaccgaaac ctacggtatt
    14221 ggcggatttg cgatggcgac cgcgcccgcg atcgtcgcgc tggtgggggg cacggtggaa
    14281 gaggctatcg atttctcccg tcagatgcgt gaaatcaccc ttggcgaaaa ccccaacgtc
    14341 accattccgc tgctcggttt tatgggcgtg ccgtcggcaa tcgacatcac ccgcgtgggt
    14401 agcagcggca ttctgccggt gatcaacacc gccattgccc ataaagatgc gggcgtcggc
    14461 atgattggcg cgggcattgt gcatccaccc ttcgcctgct tcgagaaagc cattcttggc
    14521 tggtgcgaac gttacggcgt ctgagttaca tgtttatgcc ggatgcgaca tgaatgtctt
    14581 atccggccta cgagtggcgc aagaatctgt aggcctgata agcgtagcgc atcaggcatt
    14641 ggtcaccaca gccggatgcg gcgtgaacgc cttatccggc ctacgaatgg cacgggaatc
    14701 tgtaggcctg ataagcgtag cgcatcaggc agtctggcgt tggtcataac cccatcaccc
    14761 tctgtcgcgg acataacaac atgaaagaac ttgtggtcgt tgccattggt ggcaacagca
    14821 ttatcaaaga taacgccagc cagtcgattg agcatcaggc ggaggcggtg aaagccgtgg
    14881 ccgatacggt gctggaaatg ctggcttccg attacgacat tgtgctgacc cacggcaacg
    14941 ggccgcaggt cgggctggat ttacgccgcg cggagattgc ccacgagcgc gaagggctgc
    15001 ccttaacgcc gctggcgaac tgtgtggcgg atacgcaagg tggcattggc tatctgatcc
    15061 agcaggcgct gaacaaccga ctggcgcgtc acggcgagaa aaaagccgtc accgtggtga
    15121 cccaggtgga agtggataaa aacgatccgg ggtttgccca tcccatcaaa cccattggcg
    15181 aattctttag tgaaagccag cgtgacgaac tacaaaaggc aaaccctgac tggcgttttg
    15241 ttgaagatgc cggacggggc tatcgccgcg tggtcgcctc gccggaaccg aaacgtattg
    15301 tcgaagcacc tgccattaaa gcgctgatcc aacaaggctt tgtggtgatt ggcgcgggcg
    15361 gcggtggaat tcctgtggtg cgcaccgaag cgggggatta ccaaagcgtg gacgcggtta
    15421 tcgacaaaga tctctctacc gcgctactgg ctcgcgaaat tcacgccgac attctggtga
    15481 tcaccactgg cgtcgaaaaa gtgtgtattc actttggcaa accgcagcag caggcgctgg
    15541 atcgcgtaga tattgccacc atgacccgct atatgcagga agggcatttc ccacctggca
    15601 gcatgttgcc aaaaatcatc gccagcctga cgttcctgga acaaggcggc aaagaagtga
    15661 ttatcaccac gccggaatgc ctgcctgcgg cgctacgcgg cgaaacgggc acccatatta
    15721 ttaaaaccta aggacgtaag atgaaagaaa acaatagccg ccgtgaattt ctgagccaga
    15781 gcggtaagat ggttaccgcc gccgcgctgt ttggtccctc agtgccgctc gcccatgcgg
    15841 ggggagctgg cacccgaaac tgcgaagcga acaacaccat gaaaatcact gacccgcatt
    15901 actatctcga taacgtgctg ctggaaaccg gttttgacta cgaaaatggc gtggcggtgc
    15961 agacccgcac ggcgcgccag actgtggaaa ttcagaacgg taaaattgtt gccctgcgcg
    16021 agaacaagca gcatccggac gccacgctgc cgcactatga cgctggcggt aagctgatgc
    16081 tgcccaccac ccgcgacatg catattcatc tcgacaaaac cttctacggc gggccgtggc
    16141 gctcgctcaa tcgtccggca ggcaccacta tccaggacat gatcaaactc gagcagaaaa
    16201 tgctgccgga actgcaaccg tacacgcagg aacgggcgga aaaactgatc gatttattgc
    16261 agtcgaaagg caccaccatt gcccgcagcc actgcaatat cgaaccggtt tccggcctga
    16321 aaaatctgca aaatttgcag gcggtgctgg cgcgacgtca ggcgggcttt aagtgtgaaa
    16381 tcgtcgcctt tccgcagcac ggtttgctgc tgtcgaaatc ggaatcctta atgcgcgaag
    16441 cgatgcaggc gggggcgcat tatgtcggcg ggctggaccc gaccagtgtc gatggcgcga
    16501 tggaaaaatc cctcgacacc atgttccaga ttgcgctgga ctatgacaaa ggcgtcgata
    16561 ttcacctgca cgaaaccacg ccggcaggca tggcagccat caattatatg gttgaaacag
    16621 tggagaaaac gccgcagcta aaaggcaagc tgaccatcag tcacgccttt gcgctggcaa
    16681 cgctcaacga acaacaggta gatgaactgg cgcaccggat ggtggcgcag caaatttcta
    16741 tcgcctcgac ggtgccgatt ggcacgttgc atatgccgct caaacagctg cacgacaaag
    16801 gcgtaaaagt gatgaccggc accgacagcg ttatcgacca ctggtcaccc tacggcctgg
    16861 gcgacatgct ggaaaaagcc aatctgtacg cgcagctcta tattcgccct aacgaacaga
    16921 atttatcccg ctcgctgttt ttagccaccg gtgatgtatt gccgctaaat gaaaaaggcg
    16981 agcacgtgtg gccaaaagcg caggatgacg ccagttttgt gctggtggac gcttcctgtt
    17041 ccgccgaggc ggtggcgcgt atctcgccga gaaccgcaac gttccataaa gggcaactgg
    17101 tgtgggggag tgtggcaggt tgatgcgcat ttgaaacgcc tgcaacggtg aacagtaaga
    17161 gatttaagcc ccagggagtg gggcggcctg agtatgagtg tgttggcaga aatggacatt
    17221 atctttgaag aatatgccct tattgacgtt gtgtagataa ctatttgtct acgtgaaaat
    17281 atcatcagtt ttgccgattt ttgtctttta cggatagcaa atatcacact tataggcgct
    17341 cgccttatgg ggaacgttgc tgctctgttt cattcaggct cgcggcatgt tggggctgga
    17401 tcgggtggtt taaatattgg ctatattcaa tggacgcgtt ttgccgcgat gacatatcag
    17461 gcatcgccaa atacacatag ctaatcagga gtaaacacaa tgaagatcaa agctgttggt
    17521 gcatattccg ctaaacaacc acttgaaccg atggatatca cccggcgtga accgggaccg
    17581 catgatgtca aaattgaaat cgcttactgt ggcgtctgcc attccgatct ccaccaggtc
    17641 cgttccgagt gggcggggac ggtttatccc tgcgtgccgg gccatgaaat tgtagggcgc
    17701 gtggtagccg ttggtgatca ggtagaaaaa tatgcgccgg gcgatctggt tggtgtcggc
    17761 tgcattgtcg acagttgtaa acattgcgaa gagtgtgaag acgggctgga aaactactgt
    17821 gatcacatga ccggaaccta taactcgccg acaccggatg aaccgggcca tactctgggc
    17881 ggctactcac aacagatcgt cgttcatgag cgatatgttc tgcgtattcg tcacccgcaa
    17941 gagcagctgg cggcggtggc tcctttattg tgtgcaggaa tcaccacgta ctcgccgcta
    18001 cgccactggc aggccgggcc gggtaaaaaa gtgggcgtgg tcggcatcgg cggtctggga
    18061 catatgggga ttaaactggc ccacgcgatg gggggcgcat gtggtggcat ttaccacttc
    18121 tgagtcaaaa cgcgaagcgg caaaagccct gggggccgat gaagttgtta actcacgcaa
    18181 tgccgatgag atggtggctc atgtgaagag tttcgatttc attttgaata cagtagctgc
    18241 gccacataat ctcgacgatt ttaccacctt gctgaagcgc gatggcacca taacgctggt
    18301 tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc aacctgatca tgaaacgccg
    18361 cgcgatagct ggttctatga ttggcggcat tccagaaact caggagatgc tcgatttttg
    18421 cgccgaacat ggcatcgtgg ctgatataga gatgattcgg gccgatcaaa ttaatgaagc
    18481 ctatgagcga atgctgcgtg gtgatgtgaa atatcgtttt gttatcgata atcgcacact
    18541 aacagactga aaaaattaat aaacatcctg tggtttaaca tattaacttc gctccactta
    18601 actttttagt taaggagagc gaaataatat caaagtagtg gtaaacctat agcgtaaatt
    18661 cagattgtta aattaacttc actactttac acgttagtta tgagcgattt ctgatagtgt
    18721 ctggttttat cagagcttta ttatctgcga cgtttatttt tatttaagag agccacctgc
    18781 aagttgttat tccaccagag agtgaatcgc aaagcaggtc atcatttcat ctgtatatct
    18841 gtatgccaaa tggcattccc cctccttttt cgccgatatt ttgttgtgaa cggcggagtt
    18901 tcgcttaaaa aagcaccagt agtggtttcg cagctatatg gtgtataaaa aatgttctca
    18961 tacagatgtt ttgtgaatgt gtgggttgac attcatatga aaaaaatcat aattccatca
    19021 tgtttgtgtg gggtcttttc tgtatcttac gcatcgcact caagcctgac agaaaatatg
    19081 ttgtaaggct catatcaaaa accgccatta gctcatcagg aagagcagac gacaaccagg
    19141 attgttgtat ggtacggggt tcgaggcctc gatggcggtc caacttttca aaacttttct
    19201 gaatcagatc cccttattat catccctgca aagtggaaca ttgccattgt ttggctattc
    19261 aggacgggtc agaccaatga aaaagcccac acaggggaga gtggctgaaa tgggaagcta
    19321 aagactcaag taaacttatc ggaaataagg accacgcatt acgggggcta tcgtcgccga
    19381 tggtcgggat ccggcaaatc aggttgatta cgtagagagt aaattatctg ctcaccgctg
    19441 cgtcacccct tcgtaaatca ggcgtagcgc gaatacacca ataattgcac caataacccg
    19501 actggcaacg cgttgcatac gcccataagc acgacgcact gcgggcaaag aaaacgcctg
    19561 actaagaaag actcgccaga taattgatgc gagcacaatc cccgcccagg ccattaatcg
    19621 tgcccatgtt ggtgtttcgg catgtaatgt tactgagaaa atactgataa aaaataaaac
    19681 ggtttgcggg ttagagagat cggtaattaa tccacggcgg aaaaagacat accagggggc
    19741 gctaatcggt tgttgtagtg tgctcatttg cggtgttgac aggcggtgca tgctacacca
    19801 tgcaaaccat aagagataag cgccgccgac gattctgata agcgaaaaaa tctcctcaca
    19861 ctgcgtaatt agcgttgcaa gaccaaacaa acccaacccg gaataaaatg catcgcccag
    19921 cgccacgccc agcccggtca acacccctgc gcgtcgaccg gaagccaggc tggtttgtac
    19981 taccacaaag agattggctc ccggattaaa aaaagtaatc acgaacaatc ctacggtcag
    20041 gtaaacggcg tgcaaaggat ccatagtgat ttcatccata aataagtgaa gtaacttcat
    20101 catatttcta ccaaaaataa tcgggtgcga gagagatcac aaagtgtctt atttccggtt
    20161 actggcgttt atgccctgac tgaactaatt attaatcaac ccaataatgt gggtgggtga
    20221 tagtgtgata acaactctgg agccgtaata tgaaaataat ctctaaaatg ttagtcggtg
    20281 cgttagtgtt tgccgttacc aatgtctatg ccgcagaatt aatgaccaaa gcggaatttg
    20341 aaaaggttgc atcgcaatat gaaaaaatag gtgatatttc aaccagtaat gaaatgtcga
    20401 ccgcagatgc gaaagaagat ttgatcaaaa aagccgatga aaaaggggct gatgtgttgg
    20461 tactgacctc tggtcaaact gacaataaga tccacggcac ggcaaatatt tataagaaga
    20521 agtaattctg aatcctatgt aaacatcccc gatgcgtaag tttatcggtg atcatcatct
    20581 atcgaaattt atgccggata aagcgttcgc gctgcattcg gcagttcagc ttttcagccg
    20641 ccgccagaac gtcgtccggc tgatacctaa ataattcgcc gctgctgtct tatcgccatt
    20701 aaatttctcc agtgcctgtt gtgctgtcag caagcctgga acgggagtct tcgctgactc
    20761 gcgcgccagt tccggcagta gcagctgcaa aaattgcggc gttaaatccg gcgtcgattc
    20821 cacacttaaa aatagcgcca gtcgttccat catattgcgc agttcacgaa tattgcccgg
    20881 ccagtcgtag agcaataata caatctgaca ggtctctaat ccctgacgta atgcggcaga
    20941 aaaagggaca gagagtgccg ccagagacat tttcaaaaag ctttccgcca gcggcagaat
    21001 atccgccacc cgctcgcgta gcggcggcag ttgcaggcgt aaaatactca gccgataaaa
    21061 caggtcacgg cgaaactgcc cttgctgcat atcttcttcc agattgcagt gagtggcgct
    21121 aatgacccgc acatctaccg gaacaggctg atgcccgccg acgcgggtga cctctttttc
    21181 ttccagcacc cgtaacaggc gagtctgcaa aggtagcggc atttcgccaa tctcatccag
    21241 aaacagcgta ccgccgtggg caatttcgaa cagcccggcg cgacctccgc gtcgcgagcc
    21301 ggtgaacgcc ccttcctcat agccaaacag ctctgcttcc agcagcgatt ctgcaatcgc
    21361 cccgcagttg acggcaacaa acggatgtga ctttttgccc tgtcgcgcat cgtggcgggc
    21421 aaaatattcc cgatgaatcg cctgggccgc cagctctttg cccgtccccg tttccccttc
    21481 aatcaacacc gccgcactgg agcgggcata cagcaaaata gtctgccgca cctgttccat
    21541 ctgtggtgat tgaccgagca tatcgcccag cacgtaacga gtacgcaggg cgttgcgggt
    21601 ggcatcgtga gtgttatggc gtaacgacat gcgcgtcata tccagcgcat cgctgaacgc
    21661 ctggcgtacg gtggcggcgg aataaataaa aattccggtc attccggctt cttctgccag
    21721 atcggtaatc agccccgcgc cgaccaccgc ttcggtgccg ttagctttca gctcgttaat
    21781 ctgcccgcgc gcgtcttctt cggtaatgta gctacgttgg tcgaggcgca aattaaaggt
    21841 tttttgaaac gccaccagcg ccggaatagt ttcctgataa gtgacaacgc cgatagaaga
    21901 ggtgagtttt ccggcttttg ccagtgcctg taacacatcg tagccgctcg gtttaatcaa
    21961 aataactggc actgacaggc ggcttttaag atatgcgccg ttagagccag cagcgatgat
    22021 ggcgtcgcag cgttcgcttg ccagtttctt gcggatgtag gtcaccgctt tttcaaagcc
    22081 aagctggata ggggtaatgt tcgccagatg atcaaactcg aggctgatat cgcgaaacag
    22141 ctcgaacagc cgcgttacag ataccgtcca gataaccggt ttgtcgtcat tcagccgtgg
    22201 tggatgtgcc atagcgcacc gcaaagttaa gaaaccgaat attgggttta gtcttgtttc
    22261 ataattgttg caatgaaacg cggtgaaaca ttgcctgaaa cgttaactga aacgcatatt
    22321 tgcggattac ttcatgactt tatttataac aaattgaaat taaacattta attttattca
    22381 gtcaattgtg gcacacccct tgctttgtgt ttatcaacgc aaataacaag ttgataacaa
    22441 aggatgggct atgtctctac actctccagg taaagcgttt cgcgctgcac tgactaaaga
    22501 aaatccattg cagattgttg gcaccattaa cgccaaccat gcgctgttgg cgcagcgtgc
    22561 cggatatcag gcaatttatc tctccggcgg tggcgtggcg gcagggtcgc tggggctgcc
    22621 cgatctcggt atttctactc ttgatgacgt gctgacagat attcgccgta tcaccgacgt
    22681 ttgttcgctg ccgctgctgg tggatgcgga tatcggtttt ggttcttcag cctttaacgt
    22741 ggcgcgtacg gtgaaatcaa tgattaaagc cggtgcggca ggattgcata ttgaagatca
    22801 ggttggtgcg aaacgctgcg gtcatcgtcc gaataaagcg atcgtctcga aagaagagat
    22861 ggtggatcgg atccgcgcgg cggtggatgc gaaaaccgat cctgattttg tgatcatggc
    22921 gcgcaccgat gcgctggcgg tagaggggct ggatgcggca atcgaacgag cggaggccta
    22981 tgttgaagcg ggtgccgaga tgctgttccc ggaagcaatt accgaactcg ccatgtaccg
    23041 ccagtttgcc gatgcggtgc aggtgccgat ccttgccaac attactgaat ttggtgccac
    23101 gccgctgttt accaccgacg aattacgcag cgcccatgtc gcaatggcgc tgtatcccct
    23161 ttctgctttc cgcgccatga accgcgccgc tgaacatgtc tacaacgtcc tgcgccagga
    23221 aggcacgcag aaaagcgtca tcgacaccat gcaaacccgc aacgagctgt acgaaagcat
    23281 caactactac cagtacgaag agaagctcga cgacctgttt gcccgtggtc aggtgaaata
    23341 aaaacgcccg ttggttgtat tcgacaaccg atgcctgatg cgccg
//
LOCUS       DADTFG010000075        23115 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.075, whole genome shotgun sequence.
ACCESSION   DADTFG010000075 DADTFG010000000
VERSION     DADTFG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.075"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(69..2621)
                     /gene="mrcA"
                     /locus_tag="J1138_002679"
     CDS             complement(69..2621)
                     /gene="mrcA"
                     /locus_tag="J1138_002679"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBA8454451.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            2741..3520
                     /gene="hofM"
                     /locus_tag="J1138_002680"
     CDS             2741..3520
                     /gene="hofM"
                     /locus_tag="J1138_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBA8454452.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDALSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            3520..4059
                     /locus_tag="J1138_002681"
     CDS             3520..4059
                     /locus_tag="J1138_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBA8454453.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            4043..4483
                     /locus_tag="J1138_002682"
     CDS             4043..4483
                     /locus_tag="J1138_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBA8454454.1"
                     /translation="MNMFFDWWFATSLRLRQFCWAVWLLVLVTLIFLSLTHHEESDAL
                     IRLRANHHQQWAALYRLVDTTSFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFLLSVEGDDLLFTLQLETPHEG"
     gene            4473..4877
                     /gene="hofP"
                     /locus_tag="J1138_002683"
     CDS             4473..4877
                     /gene="hofP"
                     /locus_tag="J1138_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBA8454455.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            4789..6027
                     /gene="hofQ"
                     /locus_tag="J1138_002684"
     CDS             4789..6027
                     /gene="hofQ"
                     /locus_tag="J1138_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBA8454456.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQVLAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            6428..6949
                     /gene="aroK"
                     /locus_tag="J1138_002685"
     CDS             6428..6949
                     /gene="aroK"
                     /locus_tag="J1138_002685"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBA8454457.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            7006..8094
                     /gene="aroB"
                     /locus_tag="J1138_002686"
     CDS             7006..8094
                     /gene="aroB"
                     /locus_tag="J1138_002686"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBA8454458.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKIRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            8186..9472
                     /gene="damX"
                     /locus_tag="J1138_002687"
     CDS             8186..9472
                     /gene="damX"
                     /locus_tag="J1138_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBA8454459.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            9579..10415
                     /gene="dam"
                     /locus_tag="J1138_002688"
     CDS             9579..10415
                     /gene="dam"
                     /locus_tag="J1138_002688"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBA8454460.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            10433..11110
                     /gene="rpe"
                     /locus_tag="J1138_002689"
     CDS             10433..11110
                     /gene="rpe"
                     /locus_tag="J1138_002689"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA8454461.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLNYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            11103..11861
                     /gene="gph"
                     /locus_tag="J1138_002690"
     CDS             11103..11861
                     /gene="gph"
                     /locus_tag="J1138_002690"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBA8454462.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSLNDLLPALGLPHSENQESKND"
     gene            11854..12858
                     /gene="trpS"
                     /locus_tag="J1138_002691"
     CDS             11854..12858
                     /gene="trpS"
                     /locus_tag="J1138_002691"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBA8454463.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            13018..13923
                     /locus_tag="J1138_002692"
     CDS             13018..13923
                     /locus_tag="J1138_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454464.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVILTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            13940..14302
                     /locus_tag="J1138_002693"
     CDS             13940..14302
                     /locus_tag="J1138_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA8454465.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            14386..15549
                     /locus_tag="J1138_002694"
     CDS             14386..15549
                     /locus_tag="J1138_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBA8454466.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKTGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHTEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHRGEPEIVGRYDSLGNPLEA"
     gene            15550..16776
                     /locus_tag="J1138_002695"
     CDS             15550..16776
                     /locus_tag="J1138_002695"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBA8454467.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSSTWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLPDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLALLVEMMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            16773..17651
                     /locus_tag="J1138_002696"
     CDS             16773..17651
                     /locus_tag="J1138_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBA8454468.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNHTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGVLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            17662..18015
                     /locus_tag="J1138_002697"
     CDS             17662..18015
                     /locus_tag="J1138_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBA8454469.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMVAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            18027..19331
                     /locus_tag="J1138_002698"
     CDS             18027..19331
                     /locus_tag="J1138_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454470.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASANIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            19343..20428
                     /locus_tag="J1138_002699"
     CDS             19343..20428
                     /locus_tag="J1138_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBA8454471.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETILRILRESIASI"
     gene            complement(20536..20703)
                     /locus_tag="J1138_002700"
     CDS             complement(20536..20703)
                     /locus_tag="J1138_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBA8454472.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(20955..22328)
                     /gene="cobA"
                     /locus_tag="J1138_002701"
     CDS             complement(20955..22328)
                     /gene="cobA"
                     /locus_tag="J1138_002701"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBA8454473.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(22347..>23115)
                     /gene="nirC"
                     /locus_tag="J1138_002702"
     CDS             complement(22347..>23115)
                     /gene="nirC"
                     /locus_tag="J1138_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBA8454474.1"
                     /translation="ARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVR
                     PLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMWAILPQTWLGNLVG
                     SVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMALR
                     TEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTL
                     GNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
BASE COUNT         5474 a         5792 c         6388 g         5461 t
ORIGIN      
        1 cccgcatccg gtataaacaa agtgcacttt gtcagcaaac ttaaaaaggc gctgaagcgc
       61 ctttttaatc agaacaattc ctgtgcctcg ccattatcga taatggtcgt tcccacctcg
      121 tgcaccgcct gttgtgtcgg ctgcgtacct tcgatgaaat actcttcgcg gctgttgcca
      181 ccattagcta attgcccggt gctgcgatcg atattcaccg tcacaatacc cggtggcggc
      241 gtcagcggct gctccggcac accttcaaga acggctttca tataagcgtc ccatgcaggc
      301 tgggcactct tggcaccgcc ttcgtaacct gagatctgat ctttaatcgc tccggaagcc
      361 gttgtatgac cgagattacg acggtgatca tcaaagccaa tccagaccga ggtcacaacg
      421 cccggaccgt aacccgagaa ccacgcatct ttcgaactgt tagtggtacc cgtttttccg
      481 cctatgtcat gccgttgtaa atcacgacct gctcgccagc cagtaccctg ccagcctggt
      541 tcaccaaaaa tattggtgtt caaggcactc ttaatcagga atgccagcgg tgtgttgatg
      601 acgtgcggtg cgtactcctg cgcgccagtc ttcgccacta acgcctgatt tgcctgctcc
      661 agctgcggca ttggtacaga aacattctgc tgctcgcggg agatagcgac atcttcaaca
      721 tcgttatttt ccagcacgtt cgatttctgc gtatcaccgt aaatcaccgg aatatcgcat
      781 tccgggcagg ctactttcgg tttcgcttcg aaaatcacgc cgccctgatc gttttcaatt
      841 ttgctgataa accacgggtc caccaggaag ccgccgttcg ccatgaccgc gtagccgcgc
      901 gccacctgca ttggggtgaa ggaagctgaa cccagcgcca gcgattcggt gtggacaatg
      961 ttttgtgccg ggaagccgaa gcgttgcaga tattctgcag cgtagtcgac gcccatcgcc
     1021 cgcattgcgc gtaccatcac cacgtttttc gactgaccca gcccctgacg taagcgaatt
     1081 ggaccggcat attgcggtgg tgagttcttc ggctgccagt cagaaccggc acctgcatcc
     1141 cagcgggaaa ttggcacatc gttcaacata cttgccagcg ttagaccttt atccatcgcc
     1201 gcggtgtaga ggaacggttt gatgttggaa cccacctgac gcagtgcctg ggtggcgcgg
     1261 ttaaacttgc tctgattgaa atcaaagcca ccgaccagcg ccataacggc accgttttgc
     1321 ggattgatcg acaccagcgc cgagttcact tccggcactt gcgccagcca ccatgcatcg
     1381 tcaacttgac gaacccagat ttgctgaccc gtttgcagaa catcggtcac tttacgcggc
     1441 gtcggtccct gctgagtatc cgaacggtaa ggacgcgccc agcgaacacc ttccatactc
     1501 aatacgacgg tcgacccgtc tgccagcatc gccgttgctt cctgaggatt ggcgctggtg
     1561 actgcggcag gcagcagcgg accataggtc ggcagcgcct tcagcgtatc ggtaattttg
     1621 ttgttgtccc acgccgattc gcccactttc cacagcacat ttgccgggcc gcgatagccg
     1681 tggcgcatgt cgtagtccag cacgttatta cgtaccgcct gctgtgcggc ctgctgcact
     1741 ttgcgggtga tggtggtgta aatgcgataa ccgtcttcat aggcactttc gccataacgg
     1801 ttatacatct cctggcgcac catttcgctc aggtatggcg cagagaaagc aatctccggc
     1861 gcgtgatagt tagcgttaat cgcctcagtg cgtgtctgat cgaactgttg ttgggtgata
     1921 tatccttcat ccagcatccg cgacagcacg acgttacgcc gcgcgacggc acgatccatt
     1981 gagtagagcg ggttgaaggt ggaaggcgct ttcggcagcc cggctatcac cgccatttcg
     2041 ttcaacgtca gttggtcgac cgttttaccg aaatagactt gtgccgcagc accgacacca
     2101 taggcgcggt aaccgaggta aatcttgttc agataaagct cgaggatctc gtctttcgtc
     2161 agcaactgtt caatgcgaat cgcgaggaag acttccttaa tcttgcgcat cagcgtgcgt
     2221 tctggactga ggaagaagtt tctcgccagc tgctgggtaa tagtactcgc cccttgtgac
     2281 gcgtgaccgg agaacagcgc cacgcttgcc gcacggaaga tccccaccgg gtcaacgccg
     2341 tgatgctcgt agaagcggct atcttccgtc gcgataaagg ctttcaccat ctccggcggg
     2401 atttgatcca acgtaaccgg aatacgacgt ttctcaccgt attgagcaat cagctcgcca
     2461 tcggcgctgt aaatctgcat cggaatttgc aggcgaacat cttttaatgt cgccacatcc
     2521 ggcagttgtg gctcgatgta gcggtatagg ccataaatcg agcctgctcc cagcagaatg
     2581 caacagactg caaggatcaa aaaatacttt acgaacttca ctggaaattt cccatttagt
     2641 ttcatttggg cagtttataa acaaacgcgc ggtagtataa aggcaagcca gacgcattga
     2701 tatacctgtc agagtgacgg gtgataagga gatcatcaca atggcattta agaactggca
     2761 aattggtttg catttacaac agcaagaagc ggtagcggtt gctatcgtgc ggagcgcaaa
     2821 agaatgctta ttgcaccgct ggtggcggtt gccactggag aacgacatta tcaaagatgg
     2881 gcggattgtt gatgtgcagc ggctggctaa cacgttgtta ccgtggagtc gtgaactgcc
     2941 gcagcgtcat cacattatgt tggcgtttcc cgccagtcgc acattacagc ggtcatttcc
     3001 acgcccgtcg atgtccctcg gtgagcggga gcaaatggcc tggctgtccg ggacgatggc
     3061 ccgtgaactg gatatggatc cggactccct gcgcttcgat tacagcgaag acgcactcag
     3121 tcccgcctat aacgtgactg ccgcgcaaag caaagagctg gcaacgctgc ttactctggc
     3181 agaaaggttg cgtgttcatg ttagcgcgat cactccggat gccagtgcat tacagcgatt
     3241 cctgcctttt ttaccttctc atcagcaatg tctggcctgg cgtgataacg aacagtggct
     3301 gtgggcgacg cgctatagct gggggcgcaa actggcggtg gggatgacta gcgcgaagga
     3361 gctggcggca gcgttatctg ttgatcccga aagcgtcgcg ctatgtggcg aaggcggatt
     3421 tgacccctgg gaggcggttt ctgttcgtca gccgccgcta ccgccaccag gtggagactt
     3481 tgccatcgcg ctggggctgg cgcttgggaa ggtgtactga tgaacccgcc aattaatttt
     3541 ttgccctggc gacagcaacg ccggaccgct tttcttcgtt tctggttgct gatgttcgtt
     3601 gcgcctttgc tgctggccgt cgggataacg ctaatactgc gtctgacaag caatgccgaa
     3661 gcacgcgtaa acgccgtttt gcttcaggcg gaacaacaac tcgcccgcag cttacagata
     3721 acgaaaccac gtttgctgga acgccagcag ttacgcgaac agcgtttgca acgtcagcgc
     3781 cagcgacaat ttacccgcga ctggcaatct gcgctggaag cactggcggc gcttttaccc
     3841 gaacatgcct ggctgacaac gataagctgg cagcagggaa cgctggagat caaggggctg
     3901 acaacaagca ttaccgcgtt aaacgcacta gaaacgtcac tacgccagga tgcttctttt
     3961 catctcaatc agcggggggc cacgcagcag gatgcgcagg gacgctggca atttgagtat
     4021 cagttaacaa ggaaggttag cgatgaacat gttctttgac tggtggttcg ccacatcact
     4081 ccgcctccgc cagttttgct gggcagtctg gttgctggtg ttagttacgc tcatttttct
     4141 gtcattgaca caccatgaag agagcgatgc attaattcga ctacgggcaa atcatcacca
     4201 gcagtgggct gcactgtatc gcctggtaga caccacttcc ttcagcgaag aaaaaacgct
     4261 gcccttttcg ccactggatt ttcagttatc cggcgcgcaa ctggtttact ggcatccatc
     4321 cgcgcaggga ggtgagttgg cgttgaaaac gctgtgggaa gcagtgccgt cggcatttac
     4381 acggctggca gagcgcaacg tcagcgtgag ccgttttttg ttaagcgtgg aaggtgatga
     4441 ccttttgttc acgctacaac tggagacgcc gcatgagggt taaacgctgg ttgttggcag
     4501 gcattgcatt gtgcctttta accggtatgc gtgacccttt taaaccgccg gaagatctat
     4561 gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggaga ggcgagcgca
     4621 tcatcggtgt aataaaagat gggcaaaaaa aatggcgacg ggtgcagcaa aacgatgtgc
     4681 tggaaaacgg ctggacgatc ttacagctga cgccagacgc actaacgctg ggaaccggga
     4741 caaactgcga accgccacac tggttgtggc aacggcaagg agatacaaat gaagcaatgg
     4801 atagccgcac tactgttgat gcttataccc ggcgtacagg cggcaaagcc gcaaaaagtg
     4861 acgctgatgg tggatgacgt tccggtagct caggtgttgc aggtgctggc tgaacaggag
     4921 aagttgaacc tggtggtttc gccagacgtc agcggtacgg tgtcgttaca tttaactgac
     4981 gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggattgat aacgcgccag
     5041 gagggcaaca ttctctcggt gcattccgtt gcctggcaga atgacaatat cgcccgccag
     5101 gaggcggagc agacgcgggc gcaggcaaat ctgccgctgg aaaatcgcaa tattactctg
     5161 caatacgccg acgccggaga gctggcgaaa gcgggggaga agctactgag tgccaaaggg
     5221 agtatgaccg tcgataaacg caccaatcgc cttttgctgc gagataacaa aacggcgtta
     5281 agcacgcttg aacagtgggt atcgcaaatg gatctgccgg tcgggcaggt tgagctctcg
     5341 gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggtgtgaa atggacgctg
     5401 gccgatgcgc aacaagctgg tggcgttggg caagtcacca cgcttggcag cgacctctcc
     5461 gtagcgacgg cgacaacgca tatcggtttt aacattggac gcatcaacgg acgtttactg
     5521 gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc cagtccgcgt
     5581 ctgctggcct cacatcttca gcctgccagc attaaacagg ggagcgaaat tccatatcag
     5641 gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt cctggggatg
     5701 gaagtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca catcagccag
     5761 aacgttccgg ggcaggtgct acagcaggca gatggcgaag tgctggcgat tgataagcag
     5821 gagatcgaaa cgcaggtcga ggtcaaaagc ggagaaacgt tggcgctggg cggcattttt
     5881 acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat tccctggttc
     5941 gggcaattat ttcgtcatga cggaaaagaa gatgaacgac gcgagttagt ggtgtttatc
     6001 acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt tttacgctga
     6061 acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac cgatttgaga
     6121 gttggtgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg cggcattttg
     6181 ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg tagcaaggca
     6241 gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt gccaaacccg
     6301 ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg tttcagttca
     6361 tgtcctgctt cgctctctga gcgaagcggg tttatcatta acgaatagtc ttagtagtac
     6421 cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg ccggaaaaag
     6481 cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt ccgatcaaga
     6541 gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag gcgaagaagg
     6601 cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg gtattgtgct
     6661 ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt ccgctcgtgg
     6721 cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc agcgtgataa
     6781 aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag cgttggccaa
     6841 tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg atgatcaaag
     6901 cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat tctggcttta
     6961 tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga gaggattgtc
     7021 gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt taatgaacca
     7081 gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccaa cgaaaccctg
     7141 gctcctctgt atctcgataa gatccgcggc gtacttgaac aggcgggtgt taacgtcgat
     7201 agcgttatcc tccctgacgg cgagcagtat aaaagcctgg ctgtactcga taccgtcttt
     7261 acggcattgt tacaaaagcc gcatggtcgc gatactactc tggtggcgct tggcggcggc
     7321 gtagtgggtg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt acgtttcatt
     7381 caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa aactgcggtc
     7441 aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcccgcttc agtggtggtg
     7501 gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct ggcagaagtc
     7561 atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga gaatctggat
     7621 gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg ttgtgagctg
     7681 aaagcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt actgaatctg
     7741 ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg gttacatggt
     7801 gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct cgggcagttt
     7861 agttctgccg aaacgcagcg tattataacc ctgctcacgc gggctgggtt accggtcaat
     7921 gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga caagaaagtc
     7981 cttgcgggag agatgcgctt aattcttccg ttggcaattg gtaagagtga agttcgcagc
     8041 ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc gtaacaacaa
     8101 gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca acgtggtaag
     8161 cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg agctgaaacc
     8221 cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt ctgagcgtac
     8281 tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca ctgacgatcg
     8341 ccgtccgact cgtgcgcaaa aagcgcgtaa tgaggaaccg gaaatcgaag aagaaattga
     8401 cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc gtaagcgcaa
     8461 aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggtgtcg gcattctggt
     8521 tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga cctcttccag
     8581 cgatcaaacc gcgtctggcg agaagagtat tgatcttgca ggcaatgcga ccgatcaggc
     8641 caatggcgtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc aggatgtttc
     8701 tctgccgccg atctcttcta cgccgactca agggcaaacc ccggtggcaa cggatggtca
     8761 acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg acccagccac aaaatcagca
     8821 acagttgaac aatgtggcgg tcaattctac attgccgact gaacccgcaa cggttgcgcc
     8881 tgttcgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaaccgctg aacgtccggc
     8941 cactacgcgc ccggctcgtc agcaggcggt gattgaaccg aaaaaaccgc aagcaaccgt
     9001 gaaaacggaa ccgaagccag tcgcacagac gccgaagcga actgaaccag ctgcccctgt
     9061 ggcgagcaca aaggcaccgg ctgcgacttc tacgccagca ccaaaagaga cggcgactac
     9121 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
     9181 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
     9241 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
     9301 ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
     9361 gtatgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgtccaggc
     9421 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
     9481 atctcccgca atggtttatc attgcgggcg ttgcctgaag cgctggatgc tgtcggagct
     9541 ttctccacag ccggagaagg tgtaattagt tagtcagcat gaagaaaaat cgcgcttttt
     9601 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
     9661 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
     9721 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
     9781 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
     9841 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
     9901 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
     9961 gcggtgagtt taacgtgccg tttggccgct acaaaaaacc ctatttcccg gaagcagagt
    10021 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    10081 gcatggcgcg cgcagatgat gcatctgtcg tctattgcga tccgccttat gcgccgctgt
    10141 ctgcgactgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    10201 atctggcgga gatcgctgaa ggtctggttg atcgccatat tccggtgctg atctccaatc
    10261 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    10321 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    10381 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    10441 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    10501 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    10561 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactacg gcattaccgc
    10621 ccctatcgac gtacatctga tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc
    10681 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    10741 gcaactgatt aaagaaaatg gctgtaaagc gggtttggta tttaacccgg cgacacctct
    10801 gagttatctg aattacgtga tggataagct ggatgtgatc ctgctgatgt ccgtcaatcc
    10861 tggtttcggc ggtcagtctt tcattcctca aacactggat aaactgcgcg aagtacgtcg
    10921 tcgtatcgac gagtctggct ttgacattcg actggaagtg gacggtggcg tgaaggtgaa
    10981 caacattggc gaaatcgctg cggcgggcgc ggatatgttt gttgctggtt cggcaatctt
    11041 cgaccagcca gactacaaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    11101 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tctcgacggt acgctggttg
    11161 acagtgcccc cggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttgcctg
    11221 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatatt ctgatggagc
    11281 gcgcattgac ctgggcgcgt caggaacgtg cgactctgcg taaaacaatg ggtaaaccgc
    11341 ccgttgatga cgacattccg gcagaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    11401 gctactatgg cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    11461 tgggcgcgtt gcaggctaaa ggcctgccgc taggcctggt caccaacaaa ccgacgccgt
    11521 tcgtcgcgcc gctgctcgaa gccttagata tcgccaaata cttcagcgtg gttatcggcg
    11581 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgaacgga
    11641 tgggcattgc tccacaacag atgctgtttg ttggcgactc acgcaatgat attcaggcag
    11701 caaaagcggc aggttgccca tcagttggct taacctacgg atataactac ggcgaggcta
    11761 tcgatctcag ccagcctgat gtaatttatc agtctctaaa tgaccttctg cccgcattag
    11821 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    11881 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    11941 aacatgcagg atgactacca ttgcatttac tgtatcgttg accaacacgc gatcaccgtg
    12001 cgccaggatg cacagaagct gcgtaaagcg acgctggata cgctggcgtt gtatctggct
    12061 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtgcc ggaacatgcg
    12121 cagttaggct gggcattgaa ctgctatacc tacttcggcg aactgagccg catgacacag
    12181 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tgactatccg
    12241 gtgctgatgg cagcggacat cctgctgtat caaactaatc tggtgccggt gggtgaagac
    12301 cagaaacagc acctggaact gagccgcgat atcgcccagc gtttcaacgc gctgtacggc
    12361 gatatcttta aggtgccaga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    12421 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    12481 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    12541 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    12601 ctttctgcgg taacgggcca gagcatcccg gaactggaaa aacagttcga aggcaagatg
    12661 tatggtcatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    12721 gaacgttacc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    12781 gcagaaaaag ccagcgcaca cgcttcccgt acgctaaaag cggtgtacga agcgattggt
    12841 tttgtggcga agccgtaatt taacattaat gccaaaaacc ggggaaaccc ggttttttta
    12901 tcctcatttg caataatccg aaaaaatgtg aagcgcctcg ccgtttccac atctcgctgt
    12961 tgcgccttat cttcaaattc ctgaagatat aaacattcat tttattgaaa atatattatg
    13021 cgtcgaacgt ttatcaaaaa agaaggcgtc gttatcacga cactggcccg ttatttgttg
    13081 ggtgaaaagt gcggtaatcg actgaaaacc atagatgagc tggcaactga atgccgttca
    13141 tcggttggcc tgacgcaggc cgcgttgaaa acgctggaat caagcggagc gatacggatt
    13201 gaacgccgtg ggcgcaatgg cagttatctg gtcgagatgg ataacaaagc attgctgact
    13261 catgtggata tcaacaacgt ggtatgtgcg atgcccttgc cctatacccg tttgtacgaa
    13321 ggcctggcga gcggattgaa agcccagttt gatggcattc ctttttacta tgcgcatatg
    13381 cgcggcgcgg atattcgcgt ggagtgttta cttaacggcg tgtatgacat ggcggtggtt
    13441 tcgcgactgg cggcggaaag ttatctcacg caaaaaggat tatgcctcgc gctggagttg
    13501 ggaccgcata cctacgttgg cgagcaccag ttgatttgcc gtaaaggcga gtccgcaaac
    13561 gtgaagcgcg tggggctgga taaccgttcg gcagatcaga aaatcatgac cgatgtcttt
    13621 tttggcgata gtgatgtgga acgagtcgat ctctcttatc acgagagttt acaacgcatt
    13681 gttaaaggcg atgttgacgc ggttatctgg aacgtagtgg cggaaaacga actgaccatg
    13741 ctgggattag aagcgacgcc gctcacagac gatccgcgat ttttacaggc caccgaagcc
    13801 gtgatcctga cgcgtgccga tgattaccca atgcaacaac tgctgcgtgc agttgttgat
    13861 aaacatgccc tgcttgctca tcaacagcga gtagtcagtg gggaacagga acccagttat
    13921 taaacgaaag gcatctgata tggaaaccag actcaacctg ctttgcgatg caggcgttat
    13981 tgataaggac atctgcaaag gcatgatgca ggtcgtcaat gtactggaaa cagagtgcca
    14041 tctaccggtg cgcagtgagc aaggaacgat ggcgatgaca catatggcga gtgcactgat
    14101 gcgcagtcgc cgtggtgaag aaatagagcc gctggatgac gaattgctgg cggaactggc
    14161 gcaatccagc cactggcaag ccgttgtgca attgcatcag gtattgttga aggaatttgc
    14221 actggaagtt aacccgtgtg aagaaggcta tttgctggcc aacctttatg gattatggat
    14281 ggctgctaac gaagaggttt gaagctcccg ataaatccac gtcgtacaac ctaaatattc
    14341 aaaaaaatga atatttaatt caaggaacag aaacgaggca acaagatgtt tgtagaagca
    14401 ttgaaacgcc agaatccggc gctgatttcc gccgcactaa gtttgtggca gcagggcaag
    14461 atcgccccgg acagttgggt gatcgacgtg gatcaagttc tggaaaacgg caagcggctg
    14521 attgagacgg cgcggcttta cggcattgaa ctgtatctga tgaccaaaca attcggtcgt
    14581 aatccgtggc tggcagaaaa actgctggct ctgggctaca gcggcattgt ggcggtggat
    14641 tacaaagagg cgcgagtcat gcgccgcgcc ggtttgccgg tagcgcatca ggggcatctg
    14701 gtacaaatcc cttgtcatca ggtttctgac gccgttgaac agggaaccga cgtcatcact
    14761 gtgtttaccc tcgacaaagc gcgggaaatt tctgcggcag cggtgaagac cggacgtgtg
    14821 cagtctgtgc tgctcaaagt ttatagcgac gatgattttc tttatccggg gcaggagagc
    14881 ggttttgtac agcattcgct gcacgaggtg gttgctgaaa tccagaatct gccggggcta
    14941 catttggcgg gacttaccca tttcccttgc ctgctttggg atgaggctgc cgggaaagtt
    15001 ttgccgacac cgaatcttca cacgctggta caagcacggg atcaactggc gaaatctggt
    15061 attgcgattg agcaactgaa cgcgccttca gcgaccagct gcacgtcgct gccattgctg
    15121 gcggaatacg gtgtgactca taccgaaccc ggtcatgcgc tgacgggtac tattccggca
    15181 aatcaacagg gcgatcagcc tgaacgtatc gcgatgctct ggttaagtga aatctcccat
    15241 catttccgtg gcgacagcta ctgctacggc ggcggttact accgtcgtgg tcacgcgcaa
    15301 catgcgctgg tgtttacgcc agaaaatcaa agaattactg aaacatatct caacgccgtt
    15361 gatgacagca gtatcgacta caccctgccg ctggcaggcg agcatccggt aagcagtgca
    15421 gtggtgctct gttttcgcac gcagattttt atcacccgta gcgatgtggt gctggtgtcc
    15481 ggtattcacc gtggcgaacc agaaatagtt ggtcgttatg acagcctcgg caaccctctg
    15541 gaggcgtaaa tggcgcgatt tgtggtgtta gtgattgata gctttggcgt aggggcaatg
    15601 aaagatgtca cgttggtgcg tccgcaggat gccggggcga atacctgtgg tcacatcctg
    15661 agccagttgc cgcatttgca gctaccaacg ctggagacgc tggggctaat caacgcattg
    15721 ggttatgcgc ctggcgatat gcagccgtca gactcctcca cctggggcgt ggcggaactg
    15781 caacatgaag gtggcgatac ctttatgggg catcaggaga ttttaggtac gcgcccatta
    15841 ccgccgctgc ggatgccctt tcgtgatgtg attgaccgtg ttgagcaggc attagtatcc
    15901 gccggttggc aggtggagcg tcgtggcgat gatctgcaat ttctgtgggt caatcaggcg
    15961 gttgcgattg gcgataatct cgaggcggat ttaggccagg tctataacat caccgccaat
    16021 ctctctgtga tctcttttga cgacgcaatc aaaatgggtc gtattgtgcg tgagcaggta
    16081 caggtcggtc gggtcattac atttggcggc ctgttacccg acagtcaacg cattctcgat
    16141 gccgcagaaa gcaaagaagg gcgctttatt ggcatcaacg cgccgcgttc tggcgcttat
    16201 gacaacggtt tccaggtcgt gcatatgggc tatggtgttg atgaaaaagt gcaggtgccg
    16261 caaaaactgt atgaagcggg cgtgccaacc gtgctggtgg gtaaggtggc agatatcgtc
    16321 agcaatcctt atggcgtgag ctggcaaaac ctggtggata gccagaggat tatggatatc
    16381 accctcgacg aatttaacac gcatccgaca gcgtttattt gcaccaacat tcaggaaacc
    16441 gacctcgctg gtcatgcaga agacgtcgca cgttatgccg aacgtttgca ggtcgttgac
    16501 cgtaatcttg ctctcctggt tgagatgatg cagccagatg attgcctggt cgtgatggcg
    16561 gatcacggta acgatccgac aatcggtcac agccaccata cccgtgaagt agtgccagtg
    16621 ctggtttatc agcaagggct ggtccacacg caactcggtg tgcgcaccac gctttctgat
    16681 gtgggggcta ccgtgtgtga atttttccgt gcgccaccgc cacaaaatgg tcgctctttt
    16741 ctttcctccc tccggtttgc aggagacacc ctatgagttt tgatccgacg ggctacaccc
    16801 tcgcccatga gcatctgcat attgatcttt ccggctttaa aaacaacgtg gactgccgcc
    16861 ttgatcagta tgcgttcatt tgccaggaga tgaacgacct gatggcccgg ggcgtgcgta
    16921 atgtgattga gatgaccaac cgttacatgg ggcgcaatgc gcaatttatg ctcgatgtaa
    16981 tgcgcgaaac cgggatcaac gtggtggcct gtaccggtta ttaccaggac gcgtttttcc
    17041 cggaacatgt ggcgacccgc agcgtgcagg aactggcgca ggagatggtc gatgaaattg
    17101 agcagggtat cgatggcact gacctgaaag ccggaattat cgcggagatc ggctccagcg
    17161 aagggaagat tacgtcgctg gaagagaagg tgtttattgc cgccgcgctg gcacataacc
    17221 ataccggacg cccgatctcc acgcatacgt cgttcagcac gatggggctg gagcaactgg
    17281 ctctgttgca agcccacgga gttgatcttt cgcgcgtcac cgtcggccac tgcgatctga
    17341 aagacaatct cgacaacatt ttgaagatga tcgatctcgg cgcgtatgtg cagttcgaca
    17401 ccatcggcaa gaacagttac tacccggacg aaaagcgcat tgcgatgctc catgcgctac
    17461 gtgaccgtgg ggtgctgaat cgcgtcatgc tgtcgatgga tattacgcgg cgctctcatt
    17521 taaaagccaa cggtggttat ggctatgact ttttattgac cacttttatt ccgcaattgc
    17581 gccagtcagg attcagtcag gccgatgtgg atgtgatgtt acgtgaaaat ccctctcaat
    17641 ttttccaata aggacagact catgaaaaag attggcgttg caggcttaca gcgtgagcag
    17701 attaaaaaaa ccattgaagc gacggctcct ggctgttttg aagttttcat tcacaacgac
    17761 atggaagcgg caatgaaagt gaaatccggg caactggatt actacatcgg cgcgtgtaat
    17821 accggtgcgg gcgcggcatt gtcgattgct atcgcggtga ttggctataa caaaagttgc
    17881 accattgcca agccgggcat taaagcgaaa gacgagcata tcgccaaaat ggtcgctgaa
    17941 ggaaaagtgg cgtttggcct ttccgttgag cacgtcgaac acgcgattcc gatgctgatt
    18001 aaccatctga aataaaaggc acgactatgg atctgtacat tcagattatc gtggtggcgt
    18061 gcctgacggg tatgacatcg cttctggcgc atcgctcggc agccgttttt catgacggta
    18121 tccgcccgat cctgccgcaa ctgattgaag gctatatgaa ccgtcgcgag gcggggagta
    18181 tcgcttttgg tctgagcatt ggttttgtgg cctcggtggg gatctctttt accctgaaaa
    18241 ccgggctgct caacgcatgg ttactctttc ttcctaccga tatcctcggc gtactggcga
    18301 taaacagcct gatggcgttt ggtcttggtg ctatctgggg aatattgatc ctcacctgtc
    18361 tgttgccagt aaaccagctg ctgaccgcgc taccggtgga tgtattaggt agcctggggg
    18421 aattaagctc gccggtggtt tcagcttttg cactgttccc gttggtggcg attttctacc
    18481 agtttggctg gaagcaaagt ctgatcgccg ccgtggtggt actgatgact cgtgtggtgg
    18541 tagtgcgcta tttcccacat cttaaccctg aatccatcga aatctttatt ggcatggtga
    18601 tgctgctggg gatcgcaata actcatgacc tgcgccatcg tgatgaaaat gatatcgatg
    18661 ccagcggtat gtcggtgttt gaagagcgta catcacggat tatcaaaaac ttaccgtata
    18721 tcgccatcgt gggtgcattg attgctgccg ttgccagcat gaagattttc gccggtagtg
    18781 aagtgtcgat cttcacactg gagaaagcgt actccgcagg cgtaacgccg gaacaatcgc
    18841 aaacactaat caaccaggcg gcactggcag aatttatgcg cgggctgggt tttgtgccgt
    18901 tgattgccac caccgcgtta gccaccggtg tgtatgcagt tgcgggcttt acctttgttt
    18961 atgcggtggg ctatctctcg ccgaatccga tggttgcagc ggtattaggc gcagtggtta
    19021 tttcggcgga agtcttgctg cttcgttcga tcggcaaatg gttggggcgc tacccgtcgg
    19081 tacgtaatgc gtcggctaac atccgtaacg ccatgaatat gctgatggaa gtggcgttgc
    19141 tggtcggttc gatctttgcg gcaatcaaaa tggcgggcta taccggattc tctatcgcgg
    19201 ttgccattta cttcctcaac gaatccctgg gccgtccggt acagaaaatg gcggcaccgg
    19261 tcgtagcagt aatgatcacc ggcatcctgc tgaatgttct ttactggctt ggcctgttcg
    19321 ttccggctta aggaaggcac ctatgaagac gtttcctctg caaagcctga cgcttattga
    19381 ggcgcagcaa aagcagtttg cgctggtgga cacgatttgc cgtcattttc ctggcagcga
    19441 gtttcttacc tgcggtgatt tgggcttaac gccggggcta aatcagcccc gcattaccca
    19501 gcgtgtggaa caggtgctgg ctgatgcatt tcacgcacag gctgcggcgc tggtacaggg
    19561 cgcgggtact ggcgcgatcc gtgcggcact ggcggctttg ctcaaatcgg ggcagcgtct
    19621 tctggtgcat gacgcgcctg tttacccgac gacacgggtt attattgagc agatggggct
    19681 aacgctcatt acggctgatt tcaatgatct gtcggcactg aagcaggttg ttgacgagca
    19741 acaacctgat gcagcgctgg tgcagcatac gcgccagcag ccgcaggacc gctatgtgct
    19801 ggcagacgtg ctggcaacgt tgcgcgcggc aggtgttcca gcgttaaccg atgacaacta
    19861 tgcggtgatg aaggtagcgc gaatcggctg cgaatgcggg gcgaatgtgt cgacattttc
    19921 ctgcttcaag ctatttgggc cagagggcgt tggtgcggtg gtaggcgatg ccgatgttat
    19981 tagccggatt cgcgccacgc tctattccgg cggcagtcag attcaggggg cgcaggcatt
    20041 agaggtgttg cgtggtctgg tgctcgcgcc agtgatgcac gcggtgcagg cgggggtatc
    20101 tgaacggttg ctggctttgc ttaacggcgg cgcggtggcg gaagtgaaaa gcgccgtcat
    20161 tgccaatgca cagtcgaagg tattgattgt ggagtttcat cagccgattg ccgccagagt
    20221 gctggaagag gcgcagaaac tcggtgcctt gccttacccg gttggtgcag agtcgaaata
    20281 tgaaatcccg ccgctctttt atcgactttc cggaacgttt cgccaggcga atccgcagtt
    20341 agaacattgc gcgattcgca ttaacccaaa tcgcagcggt gaagagacga tattgcggat
    20401 cttgcgtgaa agtattgcca gcatttaatc gttgtaaacg atatttttga aaagaatgca
    20461 gaaacaaata cgccctcggg taactccgag ggcgtcaggc aattaaaccg gcgttagccg
    20521 atttctgtca atggcttact gtgcagtcgg accgcaaccg ccaatgattt tagaaataga
    20581 aatagccggg tgcagcaggt agtcgtaaga gcagttctta tcacggtttt caatatgacc
    20641 agtacatgca gttaacgtag ccagtactgc gcctaccagt gcaactttaa taaatttgtt
    20701 catttacatt ccctatgtat tatatattaa ttaatattca ttgattatat gaatataagc
    20761 agactgctca ttaagaacga tacaggaata ttcaattagc gtcagtgagc gattgattta
    20821 attttgtaag tcgctgtaac ggtgaatata acccattagc agacaatatt tattaagggg
    20881 ttatgttttt atctgctgaa tatataaaga gagttgtaaa cgaaaaaggc cagacttctc
    20941 tggccttggt taaattaatg gttagagaac cagttcaatt tatcgcgcag gccaacaacc
    21001 cgaccaataa taattagcga tggactgttc atttgctgcg ccagttcgcc cagctgcgtg
    21061 agcgtaccgt caatcacgcg ctgcgtgact gccgttccgt tttcgacaat tgccaccggc
    21121 atttcaccag gcataccgta ttcaatcagc ttttgctgaa tagttgcagc ctgattcagc
    21181 cccatataaa acaccagcgt ctgtttttct gccgccaggt tttcccagtc cagctcgcca
    21241 ccggttttta agtgtccagt aattaagcgt acgctctggg cataatcgcg atgcgtgagc
    21301 ggaatacccg aataggcaga gcaaccagaa gctgcggtaa tacccggaac caccgagaat
    21361 ggaatacccg cgttgcacag tgtttccagc tcttctccgc cacgcccgaa aataaacgga
    21421 tcgccgcctt tcagacgcac cacgcgttta cctttttgcg cttcccgcag caggatctgg
    21481 ttaatctctt cctgcggtac gcagtggtat cccgcgcgtt tgccgacgaa aacgcgatca
    21541 gcatcgcggc gtaccagatt cataatatcg tcagaaacca gacggtcgta gaccaccaca
    21601 tctgcctgct gaatttgttg cagacctttc agtgtcagca gcccggcatc gcccggacct
    21661 gcaccaacta gcaccacttc accgcgatgg tcgagcggtt cattgattaa ctgttcggtg
    21721 gtttcagtaa tggctttctg atcgttgttg gccagcgact gcgccaggcg gtcgttaacg
    21781 aacaatttct cccagaaacg gcgacgctca cccatcgtgg cgaactgttg tttcactcgc
    21841 ccgcgtaatt gcccggcgta tttcgctacc tggcccagat gtaacggcag cagtgattcg
    21901 agtttttcgc gcaacaggcg cgccagaacc ggagaggtgc cgccagagga gaccgctacc
    21961 atgagcggcg agcggtcaat aatcgacggc ataataaagc tggcggcttt cggcgcatcg
    22021 accacgttac agaagatgcg acgagcttca gcggcttcgc tgacgcgctg gttaagcgcg
    22081 tcatcatctg tcgctgcaat cgccagccag caggtgtcga gaaggctttc atcaaatggc
    22141 ccttcgacga gggttaacat gcctgcatct gcccatgcgg tgaactgtgg aataaacgct
    22201 aatgcattca ccgttaagcg agcgcctgcg tctaacagca accttgcttt gcgttccgcg
    22261 acatcaccac cgccgacaat cagacagtcg cgatcgcgta attggcaaaa tataggcaaa
    22321 tgatccacgt aaaaacccct tagtaattaa ccggcagccg tttcagtttg attaaatttg
    22381 tccgcaaccg gacgattcgc tttcggcgta gcataccaat aacccaatcc catgaatacg
    22441 gcacccgata aagtattacc cagcgtcacc cacagcaggt tatgaccaat gcccgccagc
    22501 gtgtaggctt cgctgtggtt gccgaaccag gagagtgcga acagcgtcat gttagcgatg
    22561 gagtgctcgt agccggacgc gataaatgcc agcagacacc accagattgc gataaatttc
    22621 gccgcgcctt ctgtacgcaa tgccatccag atagccagac aaaccagcca gttacacaat
    22681 gcgcctttga agaataatac cattgccggt gcggtggttt tagccagcgc gacggagtga
    22741 acgatgctgg tatctaccgg cagcaggcta ccgccgcccc agctataaag catggcaacg
    22801 aagacggaac cgaccaggtt acccagccag gtttgcggca ggattgccca catttgcccg
    22861 tggctgatgg tgcccgcttt caccccaaag gtgaggaaca tggtgtgtcc ggtgaacaat
    22921 tcagaaccgg cgataatcac cagcgttaag gcgataccaa aggtcgcgcc catcaccaga
    22981 ggacgtacgg acgggtcgag caaattaccg agcgtgaaaa tcaggatgat cccaagaccc
    23041 acatacgcgc ccgccatggc ggagctgacc caaaagccga gcgggttatt tgccgacagg
    23101 cgtgcaatgc gcgca
//
LOCUS       DADTFG010000076        23062 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.076, whole genome shotgun sequence.
ACCESSION   DADTFG010000076 DADTFG010000000
VERSION     DADTFG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.076"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            249..335
                     /gene="leuL"
                     /locus_tag="J1138_002703"
     CDS             249..335
                     /gene="leuL"
                     /locus_tag="J1138_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBA8454475.1"
                     /translation="MTHIVRFIGLLLLNASFLRGRRVSGIQH"
     gene            427..1998
                     /gene="leuA"
                     /locus_tag="J1138_002704"
     CDS             427..1998
                     /gene="leuA"
                     /locus_tag="J1138_002704"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBA8454476.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQIEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            1998..3089
                     /gene="leuB"
                     /locus_tag="J1138_002705"
     CDS             1998..3089
                     /gene="leuB"
                     /locus_tag="J1138_002705"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBA8454477.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSINKANVLQSSILWREIVNEIATEYPD
                     VELTHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3092..4492
                     /gene="leuC"
                     /locus_tag="J1138_002706"
     CDS             3092..4492
                     /gene="leuC"
                     /locus_tag="J1138_002706"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBA8454478.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4503..5108
                     /gene="leuD"
                     /locus_tag="J1138_002707"
     CDS             4503..5108
                     /gene="leuD"
                     /locus_tag="J1138_002707"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBA8454479.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(5216..5347)
                     /gene="sgrT"
                     /locus_tag="J1138_002708"
     CDS             complement(5216..5347)
                     /gene="sgrT"
                     /locus_tag="J1138_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBA8454480.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5436..7091
                     /locus_tag="J1138_002709"
     CDS             5436..7091
                     /locus_tag="J1138_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HBA8454481.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVISSLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLENLTLTFYQDHIEHRVIAGIMQQILASHQVTLKIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      7125..7224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7255..8238
                     /gene="thiB"
                     /locus_tag="J1138_002710"
     CDS             7255..8238
                     /gene="thiB"
                     /locus_tag="J1138_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBA8454482.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAATNFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAEFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            8214..9824
                     /gene="thiP"
                     /locus_tag="J1138_002711"
     CDS             8214..9824
                     /gene="thiP"
                     /locus_tag="J1138_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBA8454483.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSAQLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMHGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            9808..10506
                     /gene="thiQ"
                     /locus_tag="J1138_002712"
     CDS             9808..10506
                     /gene="thiQ"
                     /locus_tag="J1138_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBA8454484.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(10620..11384)
                     /locus_tag="J1138_002713"
     CDS             complement(10620..11384)
                     /locus_tag="J1138_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA8454485.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWYAWLAGIIGCLMGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(11470..12348)
                     /gene="araC"
                     /locus_tag="J1138_002714"
     CDS             complement(11470..12348)
                     /gene="araC"
                     /locus_tag="J1138_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBA8454486.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFHQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            12687..14387
                     /gene="araB"
                     /locus_tag="J1138_002715"
     CDS             12687..14387
                     /gene="araB"
                     /locus_tag="J1138_002715"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBA8454487.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PTSFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESIVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTAQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            14398..15900
                     /gene="araA"
                     /locus_tag="J1138_002716"
     CDS             14398..15900
                     /gene="araA"
                     /locus_tag="J1138_002716"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBA8454488.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            16100..16795
                     /gene="araD"
                     /locus_tag="J1138_002717"
     CDS             16100..16795
                     /gene="araD"
                     /locus_tag="J1138_002717"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA8454489.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            16870..19221
                     /gene="polB"
                     /locus_tag="J1138_002718"
     CDS             16870..19221
                     /gene="polB"
                     /locus_tag="J1138_002718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBA8454490.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREAGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKQHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQSNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDTQL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            19386..22292
                     /gene="rapA"
                     /locus_tag="J1138_002719"
     CDS             19386..22292
                     /gene="rapA"
                     /locus_tag="J1138_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBA8454491.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            22304..22963
                     /gene="rluA"
                     /locus_tag="J1138_002720"
     CDS             22304..22963
                     /gene="rluA"
                     /locus_tag="J1138_002720"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBA8454492.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
BASE COUNT         5430 a         6278 c         6293 g         5061 t
ORIGIN      
        1 cgtgcatttt atcccccttt ccctcgttga ttagatgcaa aaaattattc tgaaatatgt
       61 caaccgatga aaagcatcgg cggttaagca gaaattaata tcgcttgttt taaccaccgc
      121 agcacaatta gctaatttta cggatgccga actcacaatg gcgtgacgtt tttattgctt
      181 cagggttgac atccgttttt gtatccagta actctaaaag catatcgcat tcatctggag
      241 ctgatttaat gactcacatc gttcgcttta tcggtctact actactaaac gcatcttttt
      301 tgcgcggtag acgagtgagc ggcatccagc attaagccag cacgcagtca aacaaaaacc
      361 cgcgccattg cgcgggtttt tttatgcccg aagcgaggcg ctcaaaaaga gacaaggacc
      421 caaaccatga gccagcaagt cattattttc gataccacat tgcgcgacgg tgaacaggcg
      481 ttacaggcaa gcttgagtgt gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg
      541 ggtgttgacg tgatggaagt cggtttcccc gtctcttcgc cgggtgattt tgaatcagtg
      601 cagaccatcg ctcgccaggt taaaaacagc cgtgtatgcg cgttagctcg ctgcgtggaa
      661 aaagatattg acgtggcagc cgaatccctg aaagtcgccg aagccttccg tatccatact
      721 tttattgcca cttcgccaat gcacatcgcc accaagctgc gcagcacgct ggacgaagtg
      781 atcgaacgcg ctatctatat ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt
      841 tcttgcgaag atgccggacg caccccaatt gccgatctgg cgcgagtggt cgaagcggcg
      901 attaatgccg gtgccaccac catcaacatt ccagacaccg tgggctacac catgccattt
      961 gagttcgccg gaatcatcag cggcctgtat gaacgcgtac ccaacatcga caaagccatt
     1021 atttccgtac atacccacga cgatttgggc ctggcggtcg gcaactcact ggcggcggta
     1081 catgccggag cacggcagat agaaggcgca atgaacggga tcggcgagcg tgccggtaac
     1141 tgttcccttg aagaagtcat catggcgatc aaagttcgta aggatattct caacgtccac
     1201 accgccatca atcaccagga gatatggcgc accagccagt tagttagcca gatttgtaat
     1261 atgccgatcc cggcaaacaa agccattgtt ggcagcggcg cattcgcaca ctcctccggt
     1321 atccaccagg atggtgtact gaaaaaccgc gaaaactacg aaatcatgac accagaatct
     1381 attggtctga accaaatcca gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa
     1441 catcgcatgg atgagatggg atataaagaa agtgaatata atttagacaa cctgtacgac
     1501 gctttcctga agctggcgga caaaaaaggc caggtgtttg attacgatct ggaggcgctg
     1561 gccttcatcg gtaagcagca agaagagccg gagcatttcc gtctggatta cttcagcgtg
     1621 cagtcaggtt ctaacgatat tgccactgcc gccgtcaaac tggcctgtgg cgaagaagtc
     1681 aaagcagaag ctgccaacgg taacggtccg gtcgatgccg tctaccaggc gataaaccgc
     1741 atcactgact ataacgtcga actggtgaaa tacagcctga ccgccaaagg ccacggtaaa
     1801 gatgcgctgg gtcaggtgga tattgtcgcc aactacaacg gtcgccgctt ccacggcgtc
     1861 ggcctggcca ccgatattgt cgagtcctcc gccaaagcca tggtgcacgt actgaacaat
     1921 atctggcgtg ccgcagaagt cgaaaaagag ttgcaacgca aagctcaaca caacgaaaac
     1981 aacaaggaaa ccgtgtgatg tcgaagaatt accatattgc cgtattgccg ggggacggta
     2041 ttggtccgga agtgatgacc caggcgctga aagtgctgga tgccgtgcgc aaccgatttg
     2101 cgatgcgtat caccaccagc cattacgatg taggcggcgc cgccattgat aaccacgggc
     2161 agccactgcc gcctgcgaca gttgaaggtt gcgagcaagc cgatgccgtg ttgtttggct
     2221 cggtaggtgg ccccaaatgg gagcatttac caccagacca acaaccggaa cgtggcgcac
     2281 tgctgccttt gcgtaaacac ttcaaattat tcagcaatct gcgtccggca aaactgtatc
     2341 aggggctgga agcgttctgc ccactgcgtg ctgacattgc cgcaaacggc tttgacatcc
     2401 tgtgtgtgcg cgaactgacc ggcggcatct atttcggtca gccaaaaggc cgcgaaggta
     2461 gcggacaata tgaaaaagcg tttgataccg aggtgtacca ccgttttgag atcgagcgta
     2521 ttgcccgcat cgcatttgaa tctgcccgca agcgtcgcca caaagtcacc tcaatcaaca
     2581 aagccaacgt gctgcaatcc tctattttat ggcgggagat cgttaacgag atcgccacgg
     2641 aatacccgga cgtcgaactg acgcatatgt acatcgacaa cgccaccatg cagctgatta
     2701 aagatccatc acagtttgac gtcttgctgt gctccaacct gtttggcgac attctgtctg
     2761 atgagtgcgc aatgatcact ggctcaatgg ggatgttgcc ttccgccagc ctgaacgagc
     2821 aaggttttgg tctgtatgaa ccggcgggcg gctcggcacc cgatatcgca ggtaaaaata
     2881 tcgccaaccc gattgcacaa attctgtcgc tggcgctgct gctgcgttac agcctggatg
     2941 ccgatgatgc ggcttccgcc attgaacgcg ccattaaccg cgcattagaa gaaggcattc
     3001 gcaccgggga tttagcccgt ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca
     3061 ttgcccgcta tgtggcagaa ggggtgtaat catggctaag acgttatacg aaaaattatt
     3121 cgacgctcac gtagtgtacg aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca
     3181 cctggtgcat gaagtgacct caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc
     3241 ggtacgtcag ccgggcaaaa ccttcgccac catggatcac aacgtctcta cccagacgaa
     3301 agacattaat gcctgcggtg aaatggcgcg catccagatg caggagctga tcaaaaactg
     3361 caaagaattt ggcgtcgagc tgtatgacct gaatcacccg tatcagggga tcgtccacgt
     3421 aatggggccg gaacagggcg tgaccttgcc ggggatgacc attgtctgcg gcgactcaca
     3481 taccgccacc cacggcgcgt ttggcgcact ggcctttggt atcggcactt ccgaagttga
     3541 acacgtactg gcaacgcaaa ccctgaaaca gggccgggcg aagaccatga aaattgaagt
     3601 ccagggcaaa gccgcgccgg gcattaccgc aaaagatatc gtgctggcaa ttattggtaa
     3661 aaccggcagc gcaggcggta ccgggcatgt ggtggaattt tgcggcgaag caatccgtga
     3721 tttaagcatg gaaggtcgta tgaccctgtg caatatggca atcgaaatgg gcgcaaaagc
     3781 cggtctggtt gcaccggacg aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc
     3841 gaaaggcaaa gatttcgacg acgccgttgc ctactggaaa accctgcaaa ccgacgaagg
     3901 agcaactttc gataccgttg tcactctgca agcagaagag atttcgccgc aggtcacctg
     3961 gggcaccaat ccaggccagg tgatttctgt gaacgacaat attcccgatc cggcttcgtt
     4021 tgccgatccg gttgaacgcg cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc
     4081 gggtattccg ctgacagaag tggctatcga caaagtgttt attggttcct gtactaactc
     4141 gcgtattgaa gatttacgcg cggcagcgga aatcgccaaa ggccggaaag tcgcgccagg
     4201 cgtacaagcg ctggtggttc ccggctccgg tccggttaaa gcccaggcgg aagcggaagg
     4261 tctggacaaa atttttatcg aagctggttt tgaatggcgc ttgccaggtt gttcaatgtg
     4321 cctggcgatg aacaacgacc gcctgaatcc gggcgaacgt tgcgcctcca ccagcaaccg
     4381 taactttgaa ggccgccagg ggcgcggcgg gcgcacgcat ctggtcagtc cggcaatggc
     4441 tgccgccgcg gctgtgaccg gacatttcgc cgacattcgc aacattaaat aaggagcaca
     4501 ccatggcaga gaaatttatc aaacacaccg gcctggtcgt tccgctggat gccgctaatg
     4561 tcgacacaga tgccattatc ccgaaacagt ttttgcagaa agtcacgcgt acgggttttg
     4621 gcgcgcatct gtttaacgac tggcgttttc tggatgaaaa aggccaacag ccaaacccgg
     4681 acttcgtgct gaacttcccg cagtatcagg gcgcttccat tttgctggca cgagaaaact
     4741 tcggctgcgg ttcctcgcgt gagcacgcgc cgtgggcatt gaccgattac ggttttaagg
     4801 tggtgattgc gccgagtttt gctgacatct tctacggcaa tagctttaac aaccagctgc
     4861 tgccggtgaa attaagcgat gcagaagtgg atgagctgtt tgcgctggtg aaagccaatc
     4921 cggggattca ttttgatgta gacctggaag cgcaagaggt gaaagcggga gagaaaactt
     4981 atcgctttac catcgatgcc ttccgccgcc actgcatgat gaacggtctg gacagcatcg
     5041 gcctgactct gcagcacgac gatgctattg cgtcttatga agagaagcaa ccggcgttta
     5101 tgcgttagtt tgtacaccgt ccggcactca atcgccaaaa agaaaaaagc cagcagataa
     5161 tcactgctgg cgtggcgatt aacaccaata ctcagtcaca catgatgtcc gcaaatcagt
     5221 agtcagaatg actcccctcc cactgcatca gctcttcaag catggttaat cgttgtggga
     5281 cgcttaacca acgcaaccag cacaacttcg ctgtcgcggt aaaatagtgc tgataaaact
     5341 gacgcatggg gcacctcctt gcttcatcgt taggaattat tgacttaata tagggaaaat
     5401 aaaattgctg tcttttgcgc aggagttccc cttttatgcc atctgctcgt ctgcaacaac
     5461 agttcatccg cctgtggcaa tgctgcgagg gtaaatctca ggagaccact ctcaacgaac
     5521 tggcagcgtt actgagttgc tcgcgtcgtc atatgcgcac cctgctcaac accatgcagg
     5581 atcgcggctg gctgacgtgg gaagcggaag tcgggcgcgg taaacgttca cgcctgacat
     5641 tcctctatac cgggctggcg cttcagcaac agcgggcgga agacctgctg gagcaggatc
     5701 gtatcgacca actagtgcag ttggttggcg acaaagcgac tgtgcggcaa atgctggttt
     5761 ctcatctggg ccgcagcttc cgccagggac ggcacatcct gcgcgtgctc tactatcgtc
     5821 cgttgcgtaa tctgctacct ggcagcgcat tgcgccgttc tgaaacccat atcgcccggc
     5881 aaatcttcag ttcgctaacg cgcataaatg aggaaaatgg ggaactggaa gcagacatcg
     5941 cacaccactg gcagcaaata tcaccgcttc actggcgttt ctttttgcgt ccaggagtcc
     6001 attttcacca tggtcgtgaa ctggaaatgg acgatgtgat ctcctcttta aaacgaatca
     6061 atacgcttcc gctctattcg catattgctg acattgtgtc gccgacgccc tggacgctgg
     6121 atatccatct cacgcaaccg gaccgctggt tgccgttact gctggggcaa gttccggcaa
     6181 tgatcctgcc gcgcgaatgg gaaaccctca gtaactttgc cagccatccc atcggcaccg
     6241 gtccgtatgc ggtgatccgc aacagcacca atcaactgaa aattcaggca ttcgatgact
     6301 tcttcggtta ccgggcatta atcgacgaag ttaacgtctg ggttctgccg gaaattgccg
     6361 acgagccagc cggagggctg atgctaaaag gtccacaggg cgaggaaaaa gagattgaaa
     6421 gccgcctgga ggaaggttgc tactatttac tgttcgacag ccgcacccat cgaggggcga
     6481 atcagcaagt cagagactgg gtaagctatg tgctttctcc gacgaatctg gtctatttcg
     6541 ctgaggaaca gtaccagcaa ctgtggttcc cggcttatgg actgctcccc cgttggcacc
     6601 atgcccgccc gacacattgc gaaaaacccg cggggctgga aaaccttacc ctgacctttt
     6661 atcaggatca tattgaacat cgggtgattg ccgggatcat gcagcagatt ctggcaagtc
     6721 accaggtcac gctgaaaatc aaagagatca gctacgatca gtggcatgaa ggagagatcg
     6781 agagcgatat ctggcttaac agcgccaact ttacgctgcc gctggatttt tcgctgttcg
     6841 cgcacttgtg cgaggtgccg ctgctccaac actgtattcc gatcgactgg caagccgacg
     6901 ccgcccgctg gcgcaatggc gaaatgaacc tggcgaactg gtgccagcaa ctggtcgcaa
     6961 gcaaagcaat ggtgccactt atccaccact ggctgatcat tcaggggcaa cgtagtatgc
     7021 gcggcctgcg catgaacacc ctcggctggt tcgattttaa atcagcgtgg tttgcaccac
     7081 cggatccatg attgctggtt gataacaaaa tcactacact aacgccgttc tcaacggggt
     7141 gccacgcagc cgcgtgcgct gagaaaatac ccgtcgaacc tgatccggat aacgccggcg
     7201 aagggatttg aggctccttc tcaagtcctt tgccactctt ttgtgaggtg caaagtgtta
     7261 aaaaaatgtc ttcccctgct gttgctgtgc acagcgcctg ttttcgctaa acctgttctg
     7321 actgtttata cctacgattc ctttgccgct gactggggac ctggtccggt agtcaaaaaa
     7381 gcctttgaag cagactgtaa ttgcgaactg aaactggtgg cgctggaaga tggcgtttcg
     7441 ctgctcaacc gtctacggat ggaaggcaaa aacagcaaag ccgatgtggt gctggggctg
     7501 gataacaatc tgttagacgc cgccagcaaa acagggctgt ttgctaaaag cggtgtggca
     7561 gcggaagccg ttaacgttcc tggcggctgg aataatgaca ctttcgtacc gtttgattac
     7621 ggctatttcg ccttcgtcta tgacaagaac aaactgaaaa atccgccgca aagcctgaaa
     7681 gaattggttg agagcgatca aaactggcgg gtgatttatg aagatccgcg taccagtaca
     7741 ccaggtctgg gtctgttgct atggatgcaa aaagtctatg gcgataacgc cccgcaagcc
     7801 tggcagaaac tggcgaagaa aactgtcacg gtcaccaaag gctggagcga agcctacggc
     7861 ctgtttttaa aaggcgaaag cgatctggta ctgagttaca ccacctctcc ggcttatcac
     7921 attctcgaag agaagaaaga taactacgcc gccacaaact ttagcgaagg tcactatctg
     7981 caggtggaag ttgccgcccg caccgctgcc agcaagcagc cagagctggc gcaaaaattc
     8041 ctccagttta tggtttctcc ggcttttcag aatgcgatcc caaccggcaa ctggatgtat
     8101 ccggtagcaa acgtcactct gcctgcagaa tttgaacaat tgaccaaacc agccaccacg
     8161 ctagagttca cacccgccga agtggcggca caacgtcagg catggattag cgaatggcaa
     8221 cgcgccgtca gccgttaatt cccggctggt taattccagg cgtaagcgcc gccacgctgg
     8281 tggtagcggt tgcactggcg gcgtttctcg ccctgtggtg gaacgcgccg cagggtgact
     8341 gggtggcagt ctggcaggac agctacctgt ggcatgtggt gcgcttctcc ttctggcagg
     8401 cgtttctctc ggcgcagctc tctgtcgtac ccgcaatatt cctcgcccgc gccctctatc
     8461 gcaggcgctt tcctgggcga cagatgctgt tgcgtctgtg cgcaatgacc ttgatcctcc
     8521 cggtgctggt tgctgttttc ggcattctta gcgtctatgg tcgccagggc tggctggcct
     8581 cgctctggca atcacttggt ctggagtgga ccttttcgcc ctacggcctg caaggcattt
     8641 tgctggccca tgtgtttttt aatctgccga tggctagccg tttattactc caggcactgg
     8701 aaaacattcc cggcgaacaa cgtcagcttg ccgcccaact tgggatgcac ggctggcatt
     8761 ttttccgttt cgtcgaatgg ccgtggttac ggcgacaaat cccgccggtt gccgcactaa
     8821 tctttatgct ctgtttcgcc agcttcgcca ccgtgctatc gctgggcggc ggtccgcagg
     8881 cgaccactat cgagctggca atctatcagg cgctgagtta cgactacgat cctgcccgcg
     8941 cggcaatgct ggcgctgatc cagatggtgt gctgcctcgg gctggtgctg ttgagtcagc
     9001 gattgagtaa ggccattgcg cctggtacca cactgctgca aggctggcgc gacccggacg
     9061 atcgtctgca tagtcgcatt tgcgacacgg cgttaattgt gctggcgctg ctgctgttgc
     9121 tgccaccgtt actggcggtg atcgtcgatg gggtaaatcg ccagttgccg gaagtgctgg
     9181 cacaaccggt gctgtggcag gcgctgtgga cctcgttgcg tattgcgctg gcggcaggcg
     9241 tattgtgcgt agtgctgacc atgatgctgc tatggagcag tcgcgaactt cgggcgcggc
     9301 agaaaatgct ggcgggtcag gcgctggaga tgagcggcat gttgatcctc gccatgccgg
     9361 ggattgtgct ggctacgggc ttctttttac tgctcaacaa cactatcggc ctgccacaat
     9421 ctgctgacgg cattgtgatt ttcaccaatg cgttaatggc aatcccttat gcgctgaaag
     9481 tgctggaaaa cccgatgcgc gatatcaccg cccgctacag catgttgtgt cagtcgctgg
     9541 gcattgaagg ctggtcacgc ttaaaagtgg tggagctgcg cgccctgaaa cgtccactgg
     9601 cgcaggcgct ggcctttgct tgtgtgctgt cgattggtga ttttggcgtg gtggcattgt
     9661 tcggtaacga tgatttccgt accctgccgt tttatctcta ccagcaaatt ggctcctatc
     9721 gcagccagga cggcgcggtc accgcgttaa ttctgctgct gctctgtttt ctgctgttta
     9781 ccgtgattga aaaaataccg gggcgaaatg ttaaaactga ctgatatcac ctggctttac
     9841 caccatttgc cgatgcgttt tagcttaacg gtggaacgcg gcgagcaggt ggctatcctc
     9901 gggccaagcg gcgcgggtaa aagtaccctg cttaatttga tcgccggttt tctgacgcca
     9961 gccagcggtt cgctgactat cgacggcgta gatcacacca cgacgccgcc gtcacgccgt
    10021 ccggtgtcga tgctgtttca ggagaacaac ctgttcagcc acctgacggt cgcgcagaac
    10081 atcgggctgg ggctaaatcc ggggttgaaa ctaaatgcgg cgcagcagaa gaaaatgcac
    10141 gcgattgccc accagatggg gattgataat ttaatggcgc ggttacctgg cgagctttcc
    10201 ggcggtcagc gacagcgcgt ggcgttagcg cgttgtctgg tacgcgaaca gcccatttta
    10261 ttgctcgatg aaccgttttc cgcactcgat ccggcgctac gtcaggagat gctgacgctg
    10321 gtgagttcga gctgccaaca gcaaaaaatg acgctcttga tggtgtcgca cagtgtggaa
    10381 gatgcggcgc ggatcgccac gcgctcggtg gtggtcgccg acgggcgtat cgcctggcag
    10441 ggtaagaccg atgagttgtt gagcggtaag gcgagtgctt cggcgctgtt gggaattaaa
    10501 ggttaatatg cactgtcgga tgcggcgtga acgccttatc cgacctacag gtagtgcgtt
    10561 ttgtaggcct gataagacgc ggcaagcgtc gcatcaggca acccgcacaa agctatcctc
    10621 taaaccccaa ccactttacg caaaatatcg atatacaccg gcatcaacgg gtggcgaatt
    10681 aacaccacca gcgccaccac gccgatggca gaaatcaacg gagtcagcca caacaaacga
    10741 ccgcgggaca aataatgact caaacggtca gtcgctttac cgctgcgcca taaccgccag
    10801 cacagccagc ctccaaccca caaaaacacc gctgttgcca gcagcaacca tttaaactca
    10861 ccgctctgca tcccggcagg aatatcgatc gccgcacccg ccagaatccc cggcaggaag
    10921 taaaacggtg gccacagcaa gcagccgata atattcggcg taataaattt tgccaccggg
    10981 agatccagca ttcccgccac cattggtacc agcggacgcg tcgggccgac aaaacgaccg
    11041 accagaatgg tgaacatgct gtgctgatgc agcgcatgtt cggttttatc gagtaatgct
    11101 ttgttcttct tcagaaatga ccagcgatgc aacggctttt taaaacgcca gcccagccag
    11161 aaggagatcc agtcgcccat caggcagcca ataatccccg ccagccaggc gtaccagaaa
    11221 cttaactcac cgctaccaat cagcgctccc agccccgcca tcagcaccgt accgggcaga
    11281 atcaaaccga ccagcgccag cgactccaga aaggccacca acaccaccgc catcaatgaa
    11341 tacacggtgg attgggtaat aaagtgttcc agcaatgctt gcataatgtg cctgtcaaat
    11401 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttatctgatt
    11461 cgttaccaat tatgacaact tggcggctac atcattcact ttttcttcac aaccggcacg
    11521 gaactcgctc gggctggccc cggtgcattt tttaaatact cgcgagaaat agagttgatc
    11581 atcaaaaccg acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
    11641 cttcgcctga ctgatgcgct ggtcctcgcg ccagcttaat acgctaatcc ctaactgctg
    11701 gtggaacaaa tgcgacagac gcgacggcga caggcagaca tgctgtgcga cgctggcgat
    11761 atcaaaatta ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
    11821 atccatcggc ggatgaagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
    11881 aagcagattt atcgccagca attccgaata gcgcccttcc ccttgtccgg cattaatgat
    11941 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaaccggt
    12001 attggcaaat atcgacggcc agttaagcca ttcatgccag taggcacgcg gacgaaagta
    12061 aacccactgg tgataccatt cgtgagcctc cggatgacga ccgtagtgat gaatctctcc
    12121 aggcgggaac agcaaaatat cacccggtcg gcagacaaat tctcgtccct gatttttcac
    12181 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
    12241 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
    12301 gttaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
    12361 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
    12421 cttttactgg ctcttctcgc taacccaacc ggtaaccccg cttattaaaa gcattctgta
    12481 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
    12541 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
    12601 cataagatta gcggatccag cctgacgctt tttttcgcaa ctctctactg tttctccata
    12661 cccgtttttt tggatggagt gaaacgatgg cgattgcaat tggcctcgat tttggcagtg
    12721 attctgtgcg agctttggcg gtggactgcg ccaccggtga agagatcgcc accagcgtag
    12781 agtggtatcc ccgttggcag aacgggcaat tttgtgatgc cccgaataac cagttccgtc
    12841 atcatccgcg tgactacatt gagtcaatgg aagcggcgct gaaaactgtg cttgcagagc
    12901 ttagcgtcga acagcgcgca gctgtggtcg ggattggcgt tgacacaacc ggctcgacgc
    12961 ccgcaccgat tgacgccgac ggtaacgtgc tggcgctgcg accggagttt gccgagaacc
    13021 cgaacgcgat gttcgtattg tggaaagacc acaccgcggt tgaagaagcg gaagagatta
    13081 cccgcttatg tcacacgccg ggcaacgttg actactcccg ctatattggc ggtatttatt
    13141 ccagcgaatg gttctgggca aaaatcctgc atgtaactcg ccaggacaac gccgtggcac
    13201 aatctgccgc atcatggatt gaactgtgcg actgggtgcc agctctgctt tccggtacca
    13261 cccgcccgca ggatattcgt cgcggacgtt gcagcgccgg gcataaatct ctgtggcacg
    13321 aaagctgggg tggcctgccg ccaaccagtt tctttgatga gctggaccca atcctcaatc
    13381 gccatttgcc ttccccacta ttcactgaca cctggactgc cgatattccg gtgggcacct
    13441 tatgcccgga atgggcgcag cgtctcggcc tgcctgaaag catagtgatt tccggcggcg
    13501 cgtttgactg ccatatgggc gcagttggcg caggcgcaca gcctaacgca ctggtaaaag
    13561 ttatcggtac ttccacctgc gacattctga ttgccgacaa acagagcgtt ggcgagcggg
    13621 cagtgaaagg tatttgcggt caggttgatg gcagcgtggt gcctggattt atcggtctgg
    13681 aagcaggcca atcggcgttt ggggatatct acgcctggtt cggtcgcgta ctcggctggc
    13741 cgctggaaca gcttgccgcc cagcatccgg aactgaaaga gcaaatcaac gccagccaga
    13801 aacaactgct tccggcgctg accgaagcat gggccaaaaa tccgtctctg gatcacctgc
    13861 cggtggtgct cgactggttt aacggccgcc gcacaccgaa cgctaaccaa cgcctgaaag
    13921 gggtgattac cgatctgaac ctcgctaccg acgctccgct gctgttcggc ggtttgattg
    13981 ccgccaccgc ctttggcgca cgcgcaatta tggagtgctt caccgctcag gggatcgccg
    14041 tcaataacgt gatggcactg ggcggcatcg cgcgcaaaaa ccaggtcatt atgcaggcct
    14101 gctgcgacgt gctgaatcgc ccgctgcaaa ttgttgcttc tgaccaatgt tgcgcgctcg
    14161 gtgcggcgat tttcgctgcc gtcgccgcaa aagtgcacgc cgatatcccc tcagcccagc
    14221 aaaaaatggc cagtgcggta gagaaaaccc tgcaaccgtg cagcgaacag gcacaacgct
    14281 ttgaacagct ttatcgccgc tatcagcaat gggcgatgag cgccgaacaa cactatcttc
    14341 caacttccgc cccggcacag gctgcccagg ccgttccgac tctataagga cacgacaatg
    14401 acgatttttg ataattatga agtgtggttt gtaattggca gccagcatct ttacggcccg
    14461 gagactctgc gccaggtgac gcaacatgcg gaacacgttg ttaatgcact gaatacagaa
    14521 gcgaagttgc cctgcaaact ggtgctgaaa ccgctgggca ccacgccgga tgaaatcacc
    14581 gctatttgcc gcgacgcgaa ttacgacgat cgttgcgccg ggctggtagt gtggctgcac
    14641 actttctccc cggccaaaat gtggatcaac ggcctgacca tgctcaacaa accgttgctg
    14701 caattccaca cccagttcaa cgcggcgctg ccgtgggaca gcatcgacat ggactttatg
    14761 aacctgaacc agaccgcgca tggaggtcgc gagttcggct tcatcggcgc gcgtatgcgt
    14821 caacaacatg ccgtcgttac cggtcactgg caggataaac aagcacatga gcgtatcggc
    14881 tcctggatgc gtcaggcggt ctctaaacag gatacccgtc atctgaaagt ctgccgtttt
    14941 ggcgataaca tgcgtgaagt agcggtcacc gatggcgata aagttgccgc acagatcaag
    15001 ttcggtttct ccgtcaatac ctgggcggtt ggcgatctgg tgcaggtggt gaactccatc
    15061 agcgacggcg atgttaacgc gctggtcgat gagtacgaaa gctgctacac catgacacct
    15121 gccacacaaa tccacggcga aaaacgacag aacgtgctgg aagcggcgcg tattgagctg
    15181 gggatgaagc gtttcctgga acaaggtggc ttccacgctt tcactactac ctttgaagat
    15241 ttgcacggcc tgaaacagct tccgggtctg gccgtacagc gtctgatgca gcagggctac
    15301 ggctttgcgg gcgaaggcga ctggaaaact gccgccctgc ttcgcatcat gaaggtgatg
    15361 tcaaccggtc tgcagggcgg cacctccttt atggaggact acacctatca cttcgagaaa
    15421 ggtaatgacc tggtactcgg ctcccatatg ctggaagtct gtccgtcgat cgctgtggaa
    15481 gagaaaccga tcctcgacgt tcagcatctc ggtattggcg gtaaagacga tcctgcccgc
    15541 ctgatcttca acactcaaac cggtccagcc attgtcgcca gcctgattga tctcggcgat
    15601 cgttaccgtc tgctggttaa ctgcatcgac acggtgaaaa caccgcactc cctgccgaaa
    15661 ctgccggtgg cgaatgcgct gtggaaagcg caaccggatc taccaaccgc ttccgaagcg
    15721 tggatccttg ctggtggcgc gcaccatacc gtcttcagcc atgcgctgaa cctcaacgat
    15781 atgcgccagt ttgccgagat gcacgacatt gaaatcacgg tgattgataa cgacacccgc
    15841 ctgccagcgt ttaaagacgc gctgcgctgg aacgaagtgt attacgggtt tcgtcgctaa
    15901 gtagccgcat caggtatgta acgcctgatg cgacgctgac gcgtcttatc aggcctacgc
    15961 gttccggatt tgtaggccgg ataagcaaag cgcatccggc attcaccgcc tgatgcgacg
    16021 ccgacgcgtc ttatcaggcc tacacgctgc gattttgtag gccggataag caaagcgcat
    16081 ccggcacgaa ggagtcaaca tgttagaaga tctcaaacgc caggtcttag aagccaacct
    16141 ggcgctgcca aaacacaacc tggtcacgct cacctggggc aatgtcagcg ccgttgatcg
    16201 cgggcgcggc gtcctggtga tcaaaccttc cggcgtcgac tacagcatca tgaccgctga
    16261 cgatatggtc gtggtcagca tcgaaaccgg tgaagtggtt gaaggtacga aaaagccctc
    16321 ctccgacacg ccaactcacc gactgctcta tcaggcattc ccgtctattg gcggcattgt
    16381 gcacacacac tcgcgccacg ccaccatctg ggcgcaggcg ggccagtcaa ttccggcaac
    16441 cggtaccacc cacgccgact atttctacgg tcccattccc tgcacccgca aaatgaccga
    16501 cgcagaaatc aacggtgaat atgagtggga aaccggtaac gtcatcgtag aaaccttcga
    16561 aaaacagggt atcgatgcag cgcaaatgcc cggcgtgctg gtccattctc acggcccatt
    16621 tgcatgggga aaaaacgccg aagatgcggt gcataacgcc atcgtgctgg aagaagtcgc
    16681 ttatatgggg atattctgcc gtcagttagc gccgcagtta ccggatatgc agcaaacgct
    16741 gctggataaa cactatctgc gtaagcatgg cgcgaaggca tattacgggc agtaatgact
    16801 gtataaaacc acagccaatc aaacgaaacc aggctataat caagcctggt tttttattgg
    16861 attttcagcg tggcgcaggc aggttttatc ttaacccgac actggcggga caccccgcaa
    16921 ggaaccgaag tttccttttg gttagcgacg gacaacgggc cgttgcaggt tacgcttgcg
    16981 ccgcaagagt ccgtggcgtt tattcccgcc gatcaggttc cccgcgccca gcatattttg
    17041 cagggtgagc aaggctttcg cctgacgccg ctggcgttaa aggattttca tcgccagccg
    17101 gtgtatggcc tttactgtcg cgcccatcgc cagttgatga attacgaaaa gcgcctgcgt
    17161 gaagctggcg ttaccgtcta cgaggccgat gtgcgcccgc cagaacgcta tctgatggag
    17221 cggtttatca cctcgccagt gtgggtcgag ggtgatatgc gcaacggcgc tatcgttaat
    17281 gcccgtctga aacagcatcc cgactatcgt ccgccgctca agtgggtttc tattgatatt
    17341 gaaaccaccc gccacggtga gctgtactgc atcggcctgg aaggttgcgg gcagcgtatc
    17401 gtttatatgc tggggccaga gaatggcgac gcctccgcgc tcgattttga actggaatac
    17461 gtcgccagcc gcccgctgct actggaaaaa cttaacgcct ggtttgcgac tcatgatcct
    17521 gatgtgatca tcggttggaa cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc
    17581 gagcgttacc gtattccgct gcgtctgggg cgtgataaca gcgagttgga gtggcgcgag
    17641 cacggcttta aaaacggcgt cttttttgct caggccaaag ggcgactgat tatcgacggt
    17701 atcgaggcgc tgaaatccgc gttctggaat ttctcttcat tctcgctgga aaccgtcgct
    17761 caggagttat taggcgaagg aaaatctatc gataacccgt gggatcgaat ggacgaaatt
    17821 gaccgccgtt tcgccgaaga taagcctgcg ctggcaactt ataacctgaa agattgcgag
    17881 ctggtgacgc agatcttcca caaaactgaa atcatgccgt ttttgctcga acgggcgacg
    17941 gtgaacggcc tgccggtaga tcgacacggc ggttcggtgg cagcgtttgg tcatctctat
    18001 tttccccgta tgcatcgcgc tggttatgtc gcgcctaatc tcggcgaagt gccgccgcac
    18061 gccagccctg gcggctacgt gatggattcg cgacctgggc tttatgattc ggtgctggtg
    18121 ctggactaca aaagcctgta cccgtcgatc atccgcacct ttctgattga tcccgtcgga
    18181 ctggtggaag gcatggcgca gcctgatcca gagcacagta ccgaaggttt tctcgatgcc
    18241 tggttctcac gggagaaaca ttgcctgccg gagattgtga ctaacatctg gcacgggcgc
    18301 gatgaagcca aacgtcagag taataagccg ctttcgcagg cgctgaagat cattatgaat
    18361 gccttttatg gtgtgctcgg caccaccgcc tgccgtttct tcgatccacg cctggcatcg
    18421 tcgatcacca tgcgtggtca tcagatcatg cggcaaacca aaacgttgat tgaagcgcag
    18481 ggctatgatg ttatctacgg cgataccgac tcaacgtttg tctggctgaa aggtgcacat
    18541 tcggaagaag aagcagcgaa aatcggtcgt gcactggtgc agcacgttaa cgcctggtgg
    18601 gcggaaacgc tgcaaaaaca acggctgacc agcgcactgg aactggagta tgaaacccat
    18661 ttctgccgtt ttctgatgcc aaccattcgc ggggccgata ccggcagcaa aaagcgctac
    18721 gccggactga ttcaggaggg cgacaaacag cggatggtgt ttaaagggct ggaaaccgtg
    18781 cgcaccgact ggacgccgct ggcccagcag tttcagcagg agctgtacct gcgtatcttc
    18841 cgcaacgagc catatcagga atacgtgcgc gaaaccatcg acaagctgat ggcgggcgaa
    18901 ctggacacgc agctggttta ccgtaaacgc cttcgccgtc ctctgagcga atatcagcgt
    18961 aatgttccgc ctcatgtacg cgccgctcgc cttgccgatg aagaaaacca taagcgcggt
    19021 cgccccttgc aatatcagaa ccgcggcacc attaagtacg tatggaccac caacggcccg
    19081 gagccgctgg actaccaacg ttcaccactg gattacgaac actatctgac ccgccagcta
    19141 caacccgtgg cggagggaat actccctttt attgaggata attttgctac acttatgacc
    19201 gggcaacttg ggctattttg agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg
    19261 acgaatgctt tgccatccag taccatagcg ccctttccat tcctggacct gaataacacc
    19321 actacctcat aagcacggta gcgggtggtt attgcctgca attaaagata tagagccgaa
    19381 cacatatgcc ttttacactt ggtcaacgct ggatcagcga tacagaaagc gaattgggac
    19441 ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt acttttccca tctactggtg
    19501 aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg cgtgatgttc aaccctggtg
    19561 ataccattac cagccatgac ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg
    19621 gcttgctgac ctatatcggt actcgccttg atactgaaga gtccggcgta gccctgcgtg
    19681 aagttttcct tgatagcaaa ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc
    19741 agattgaccg tatggaccgc tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac
    19801 agttccgtat gccgtacagc ggcctgcgcg gtcagcgtac cagcctgatc ccgcaccagc
    19861 tcaacatcgc tcatgatgtt ggtcgccgcc acgcgccgcg cgtcctgctg gctgacgaag
    19921 tgggtttagg gaaaaccatt gaagccggga tgatcctgca tcagcaactg ctctctggcg
    19981 ctgctgaacg tgtgctgatt atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa
    20041 tgctgcgccg tttcaacctg cgctttgctc tgtttgatga tgagcgttat gccgaagctc
    20101 agcacgatgc ttacaacccg ttcgataccg aacagctggt gatttgctcg ctggattttg
    20161 cccgtcgtag caaacagcgt ctggaacatc tctgtgaagc cgaatgggat ctactggtgg
    20221 tcgatgaagc gcatcacctg gtgtggagcg aagatgcgcc gagccgtgaa tatcaggcca
    20281 ttgaacaact ggcagagcac gtgccgggcg ttctgctgct gaccgcaacc ccggaacagc
    20341 tggggatgga aagccacttc gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt
    20401 ttgcgcaatt cgttgaagag cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc
    20461 tgctggctgg taacaaactc agcaatgacg aactgaacat gctcggtgag atgatcggcg
    20521 agcaggatat cgagccgctg ttgcaagcag caaacagcga cagcgaagat gcccagagcg
    20581 cccgtcagga actggtttcg atgctgatgg atcgccacgg taccagccgc gtgctgttcc
    20641 gtaacacccg taacggtgtg aaaggcttcc cgaaacgcga gctgcatact attaagctgc
    20701 cactgccgac gcagtatcag accgctatta aagtctccgg cattatgggc gcacgtaaaa
    20761 gtgcggaaga tcgcgctcgc gatatgctct acccggagcg tatttatcag gaatttgaag
    20821 gcgataacgc cacctggtgg aacttcgatc cgcgcgttga atggctgatg ggctacctga
    20881 ccagccatcg ctctcagaaa gtgctggtga tctgcgccaa agcggccact gcgctgcaac
    20941 tggagcaggt actgcgcgaa cgtgaaggta ttcgcgccgc agtgttccac gaaggcatgt
    21001 cgattatcga acgtgaccgc gctgccgcct ggtttgccga agaagacacc ggcgcacagg
    21061 tactgctgtg ctcggaaatc ggttctgaag gacgtaactt ccagttcgcc agccacatgg
    21121 tgatgtttga cctgccattc aacccggatc tgctggagca gcgtattggt cgtctggatc
    21181 gtatcggtca ggcgcacgat attcagatcc atgtgcctta tctggaaaaa acggctcagt
    21241 cggtgctggt gcgctggtat cacgaaggtc tggatgcatt cgagcacacc tgcccgaccg
    21301 gacgcactat ctacgatagc gtgtacaacg atttgattaa ctatctggcc tcgccggatg
    21361 agaccgaagg ctttgacgat ctgatcaaaa actgccgcga gcagcatgaa gcgctgaaag
    21421 cacaactgga acaggggcgt gaccgcctgt tggagatcca ctccaacggt ggcgaaaaag
    21481 cccaggcact ggcagaaagc attgaagagc aggatgacga taccaacctg atcgccttcg
    21541 ccatgaacct gttcgatatt atcggtatca atcaggacga tcgcggcgac aacatgatcg
    21601 tactgacgcc gtccgatcat atgctggtgc cggacttccc tggcctgtcg gaagacggta
    21661 ttaccatcac ctttgatcgt gaagtggcgc tggcgcgtga agatgcgcag tttattacct
    21721 gggagcatcc gctgatccgc aacggtctgg atctgatcct ttctggcgat accggtagca
    21781 gcacgatttc actgttgaaa aacaaagcgt tgccggtagg tacgctgttg gtggaactga
    21841 tttatgtggt tgaagcccag gctccgaagc agttgcagct caaccgcttc ctgccaccga
    21901 cgccggtacg tatgctgctg gataaaaacg gcaacaacct ggcggcgcag gtagagtttg
    21961 aaacctttaa ccgccagctt aacgcggtta accgtcacac cggcagcaag ctggttaacg
    22021 cggtgcagca ggatgttcac gctatccttc agctggggga agcgcagatc gagaaatctg
    22081 cccgtgcatt gattgatgca gcgcgtaacg aagccgacga aaaactgtct gccgagctgt
    22141 ctcgtctgga agcgctgcgt gcagttaacc cgaacattcg ggacgacgaa ctgaccgcca
    22201 ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca ggcaggctgg cgtctggatg
    22261 ccctgcgttt gatcgttgtg acgcatcagt aacggagccg aagatgggga tggaaaacta
    22321 caatccgccg caggaaccct ggctggttat cctgtatcag gatgaccaca ttatggtggt
    22381 caacaagccg agcggtttgt tgtctgtgcc ggggcgtctg gaagagcaca aagacagcgt
    22441 gatgacgcgc attcagcgtg attatccgca ggcagaatcg gtgcatcgtc tggatatggc
    22501 taccagcggc gtgattgtag tggcgctgac caaagccgcg gagcgggagt taaaacgcca
    22561 gttccgcgag cgcgagccga aaaaacagta tgtggcccgc gtctgggggc atccatcccc
    22621 tgcggaaggt ctggtggatc tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa
    22681 agtctgttac gaaacaggta aacctgcgca gacggagtat gaagtggtgg aatatgcggc
    22741 ggataacacg gcaagagtgg tgttaaaacc gattaccggg cgttcgcatc agctgcgtgt
    22801 gcatatgctg gcgctgggtc atccgattct cggcgatcgt ttttatgcat caccagaagc
    22861 gagagcaatg gcaccgcgtt tgttgctaca tgcagagatg ctgacgatta cccatccggc
    22921 gtatggcaat agtatgacgt ttaaagcacc agcggatttt taatagtcac gctggcgaag
    22981 ttcggtaggc cggagaaggc gttcacgccg catccggcaa ttttgcgccg attgtctgat
    23041 gctgctcgcc ggatgcgacg ct
//
LOCUS       DADTFG010000077        22842 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.077, whole genome shotgun sequence.
ACCESSION   DADTFG010000077 DADTFG010000000
VERSION     DADTFG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.077"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            406..1623
                     /locus_tag="J1138_002721"
     CDS             406..1623
                     /locus_tag="J1138_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBA8454493.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1707..2306
                     /gene="yfbR"
                     /locus_tag="J1138_002722"
     CDS             1707..2306
                     /gene="yfbR"
                     /locus_tag="J1138_002722"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBA8454494.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2365..4197)
                     /locus_tag="J1138_002723"
     CDS             complement(2365..4197)
                     /locus_tag="J1138_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBA8454495.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4284..4934)
                     /gene="hxpA"
                     /locus_tag="J1138_002724"
     CDS             complement(4284..4934)
                     /gene="hxpA"
                     /locus_tag="J1138_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HBA8454496.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4945..5439)
                     /locus_tag="J1138_002725"
     CDS             complement(4945..5439)
                     /locus_tag="J1138_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBA8454497.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5522..5977)
                     /locus_tag="J1138_002726"
     CDS             complement(5522..5977)
                     /locus_tag="J1138_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBA8454498.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6315..7517
                     /gene="ackA"
                     /locus_tag="J1138_002727"
     CDS             6315..7517
                     /gene="ackA"
                     /locus_tag="J1138_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBA8454499.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7592..9736
                     /gene="pta"
                     /locus_tag="J1138_002728"
     CDS             7592..9736
                     /gene="pta"
                     /locus_tag="J1138_002728"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBA8454500.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9926..11446
                     /gene="yfcC"
                     /locus_tag="J1138_002729"
     CDS             9926..11446
                     /gene="yfcC"
                     /locus_tag="J1138_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBA8454501.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11479..12021)
                     /gene="yfcD"
                     /locus_tag="J1138_002730"
     CDS             complement(11479..12021)
                     /gene="yfcD"
                     /locus_tag="J1138_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBA8454502.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12079..12630)
                     /gene="yfcE"
                     /locus_tag="J1138_002731"
     CDS             complement(12079..12630)
                     /gene="yfcE"
                     /locus_tag="J1138_002731"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBA8454503.1"
                     /translation="MKLMFASDIHGSLPATERVLELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12686..13330)
                     /gene="yfcF"
                     /locus_tag="J1138_002732"
     CDS             complement(12686..13330)
                     /gene="yfcF"
                     /locus_tag="J1138_002732"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBA8454504.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13466..14113
                     /gene="yfcG"
                     /locus_tag="J1138_002733"
     CDS             13466..14113
                     /gene="yfcG"
                     /locus_tag="J1138_002733"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBA8454505.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGLALLKAQHGDERSDS"
     gene            14170..14532
                     /gene="folX"
                     /locus_tag="J1138_002734"
     CDS             14170..14532
                     /gene="folX"
                     /locus_tag="J1138_002734"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBA8454506.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14553..15446
                     /locus_tag="J1138_002735"
     CDS             14553..15446
                     /locus_tag="J1138_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HBA8454507.1"
                     /translation="MNIVITGGTGLIGRYLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAGQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15605..16378)
                     /gene="hisP"
                     /locus_tag="J1138_002736"
     CDS             complement(15605..16378)
                     /gene="hisP"
                     /locus_tag="J1138_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBA8454508.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(16386..17102)
                     /locus_tag="J1138_002737"
     CDS             complement(16386..17102)
                     /locus_tag="J1138_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8454509.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17099..17785)
                     /locus_tag="J1138_002738"
     CDS             complement(17099..17785)
                     /locus_tag="J1138_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA8454510.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(17875..18657)
                     /gene="hisJ"
                     /locus_tag="J1138_002739"
     CDS             complement(17875..18657)
                     /gene="hisJ"
                     /locus_tag="J1138_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBA8454511.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRIGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(18878..19660)
                     /gene="argT"
                     /locus_tag="J1138_002740"
     CDS             complement(18878..19660)
                     /gene="argT"
                     /locus_tag="J1138_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBA8454512.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(19926..20495)
                     /locus_tag="J1138_002741"
     CDS             complement(19926..20495)
                     /locus_tag="J1138_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBA8454513.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(20590..22107)
                     /gene="purF"
                     /locus_tag="J1138_002742"
     CDS             complement(20590..22107)
                     /gene="purF"
                     /locus_tag="J1138_002742"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBA8454514.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22144..22632)
                     /gene="cvpA"
                     /locus_tag="J1138_002743"
     CDS             complement(22144..22632)
                     /gene="cvpA"
                     /locus_tag="J1138_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBA8454515.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
BASE COUNT         5755 a         6059 c         5583 g         5445 t
ORIGIN      
        1 agcatcatcc ccattcatta attccgctta acaatagttt cacgccttgc ccccattgtg
       61 acacctcgga aatcatcacg ctgttaaact ctccgtaatg cggaaatatc atcatcgcga
      121 ttcagtagtg aattcttaaa cgaggcattt tcacacaatt atcttacaga taaaaaaacc
      181 acacttacaa ttaagaatca gaacaattca ctacataaca ttgctcatta agcatatgca
      241 cctcattatt ttgccattat taagttatta acagcacaat cgagccttcc cctctggcaa
      301 aatcttattc tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac
      361 ttccacggca gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa
      421 aaatccagca aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca
      481 aaacgcctgg aagaagaagg taacaaggta ctgaaactga acatcggcaa ccctgccccg
      541 ttcggttttg acgcgccaga tgaaattctc gttgacgtga tacgcaacct gcctaccgct
      601 caagggtatt gcgattccaa aggtctttac tccgcgcgta aagccatcat gcagcactac
      661 caggctcgtg gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg
      721 gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct
      781 gcacctgatt acccactatg gaccgcagcg gtttcgctct ccagcggtaa agcggtgcat
      841 tatctttgcg atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt
      901 acgcctcgta cgcgtgggat cgttattatc aacccaaata acccaaccgg cgcggtatat
      961 tccaaagagc ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc
     1021 gccgatgaaa tttatgacaa aattctctac gacgacgctg agcatcactc cattgcgccg
     1081 ctggcacctg acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca
     1141 ggcttccgtc aggggtggat ggtattgaac gggccgaaaa aacacgccaa aggttacatc
     1201 gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc
     1261 attcagaccg cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggaggtcgt
     1321 ctttatgagc agcgtaaccg cgcgtgggaa ttgatcaacg atattccggg cgtttcctgc
     1381 gtgaaacctc gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt
     1441 cacgacgatc agaaaatggt gctggatttc ctgttgcagg aaaaagtcct gttggtgcaa
     1501 gggacggcat tcaactggcc gtggccggat cacttccgca ttgtcacgct accgcgtgtc
     1561 gatgatatcg agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg
     1621 taatcttaat ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg
     1681 atgatgtcat catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc
     1741 gcctgaaact cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg
     1801 aacacagttt gcaggtagcg atggtcgccc atgcgctggc agctattaaa aatcgtaaat
     1861 ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcttcag
     1921 aagtgctcac cggagatctc cctactccgg tgaaatactt caattcgcaa atcgctcagg
     1981 aatacaaggc tattgaaaaa atcgctcagc aaaaactggt cgatatggtt ccggaagagc
     2041 tgcaggatat ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc
     2101 tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg tctggaagag ctcgcggccg
     2161 gaaataatga attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca
     2221 gccaggagat ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg
     2281 agattagcca ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca ggcaataagc
     2341 accggatgcg acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca
     2401 aaccgccatc acgataatgg tgaacggtac ccccaactta acaaagtcac tgaagctgta
     2461 attccccgga cctaaaacca gtgtgttaac cggtgaagat atcggtgtca taaaggcggc
     2521 ggatgctgcc atcgccacga ccatcgcaaa tggataaggc gacaccccca tcgttttggc
     2581 agcagccagc gcaatcggag ccatcaacac cgccgtcgcg gtattagaaa taaatagccc
     2641 aataaccgcc gacaagacaa acaaacaacc cagcatcata tgtggcccat aaccgccgcc
     2701 aatgtccatc agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc
     2761 aaaaggcatc atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct
     2821 ttcggcatcg atacagcgga atttccccat cagcaggcag gcgatgatag cggcaacagg
     2881 attaggaatt tcatcggtca gcatcaacgc caccattagc accagacaga aaatggcatg
     2941 gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac
     3001 gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc cagttaccaa caaccagaat
     3061 gatatcgccc agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg
     3121 ctttagcccc actacattca atccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt
     3181 accaataagt tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg
     3241 gtcagaaaaa tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg
     3301 ccggagatcg acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc
     3361 agaaacccca ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat
     3421 gacgttagca ccataacgtt cacgtaattt gagatcgtcc agccgttgac caatcattgg
     3481 cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag
     3541 atcgcgaaag gttcggcgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag
     3601 catgaaacgc atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg
     3661 tgttacgcta aagaaactga agccgtgata gccttcacgc agcaattcac tgttgactac
     3721 caggttcggc ggcgtcgcca ccagcgtcat catgccgctt atcagcccgg caaaacttaa
     3781 cggcatcatc agacgcgacg gcgacgtttg catacgcatg gcaacgctta acaccacggg
     3841 aataaagata gcaacaacgc cggttgaact cataaacgcg ccaagccccg cgacggtcag
     3901 catcaacaaa accaacattt tgatttcact gttgcccgcc actttgacca gccatgtccc
     3961 cattacagtg gcaacaccgg tacggaccaa accatcgcca ataatgaaca aggcggcaat
     4021 caggacaacg ttaggatcgg aaaagccgga aaatacttcg gggaccgtca gcgttccgct
     4081 taatgcaaac gcgacaataa caaacaaagc gactgcatcc atacgcactc tgcccgtcgc
     4141 aaacaagacg atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac
     4201 ttatccttgt taattgaggg ggataacttg attctgccat aaaaaagccc cgacgagacg
     4261 gggctaaatc atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc
     4321 acagtaattt gctccagact gtggaggact aaatcgactt cattcaggcg cggggtatct
     4381 gccggggcat taaccgcaat aacatggcaa cccgccgcca gacctgaaag cacgccagcg
     4441 ggagcatctt ccaccacaac acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac
     4501 agatacgcat caggttctgg ttttccgcgc ttcacccgct cagcggttac aaacacctct
     4561 ggcgcgggaa gccccgctat tttatgacgc gctcgcgcta ccggcatgga gccagaagtc
     4621 acaatagccc acggaatacc tgctttattc aaatgattga gtaaggcgat tgcccccgga
     4681 agcgcggtaa tgccttcggt ttccgtggcc tcgatgtgct ccagacgcgt aaactcggcg
     4741 gcaatgtctg cctcggattt gcccgccata aaatggcgca gagaggtgat cgcctgttta
     4801 ccgtgaatga aagccagcac ctcttccggc gataacccat gacgtctggc ccagttgctc
     4861 cacgcccgtt ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa
     4921 cctttgcacc gcacgcgggc ctcctcaggc gttaataatt tgattaattt cgttggcgct
     4981 caaatggtac tgacgcgggc aggcatgcca gacattaagc atgcgctgat atttttccca
     5041 cattggcgtc tgggcgttaa aaccgtgagt tccagcgtca aaatgggtat agcgcccttc
     5101 cacattaacc ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc
     5161 gaggaaggtg acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac
     5221 atgcaacgca tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag
     5281 ctcgccaaac tcgcgatcca gttcacgcat ctgtaatcca taaccacgct caatgattgt
     5341 ttgcaggcga cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg
     5401 gttagacaaa ataagacgtt gcgcgttggt catttccatt attgactcct gtatcactct
     5461 actacggtga aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca
     5521 attacaaatc atcaaggaaa gttttatcca gttgtttgaa ggcacgctta agcgtgtcag
     5581 ctaatgcctg gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca
     5641 atttaccgcg aacttcataa aaccagttga ggattgcagg aggtaatggc gttacagaac
     5701 gcttgcccag ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagc gcagtggcaa
     5761 ctgccggccc aagctgaccg cccagagcaa tctgccagca gagagtaaat acggcgatcg
     5821 gcggcataaa acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagactg
     5881 gggcaaggcg tttttccagc ggccacgtct ttgagtaatg ctgtccccgg cgaaacaagc
     5941 taaaaaaatt aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata
     6001 aagattcaaa aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc
     6061 ttccataccc actatcaggt atcctttagc agcctgaagg cctaagtagt acatattcat
     6121 tgagtcgtca aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga
     6181 ctcaagatat ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat
     6241 gtcggtgtca tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa
     6301 ttataggtac ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac
     6361 tgaaatttgc catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat
     6421 gtttccacct gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg
     6481 ctttaggtgc aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg
     6541 cacaaaaacc agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg
     6601 gcgaaaagta taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg
     6661 cagcttcttt tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga
     6721 aatctttccc acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga
     6781 ctatgccgga agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca
     6841 tccgtcgtta cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa
     6901 tgctgaacaa accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt
     6961 ccgtttctgc tatccgcaac ggtaaatgcg ttgacacctc tatgggcctg accccgctgg
     7021 aaggtctggt catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc
     7081 acgacaccct gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc
     7141 tgctgggtct gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga
     7201 aagaagacgc gaagcgcgca atggacgttt actgccaccg cctggccaaa tacatcggtg
     7261 cctacactgc gctgatggat ggtcgtctgg acgctgttgt attcaccggt ggtatcggtg
     7321 aaaatgccgc gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag
     7381 ttgatcatga acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag
     7441 gtacccgtcc tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga
     7501 gccgcctgac tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg
     7561 ccaaatcggc ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac
     7621 cggaaccagc gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa
     7681 aggcgttcgt ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc
     7741 cgatcagact acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct
     7801 gaaaatgagc tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga
     7861 gatcatcgcg aactaccacg ctaacaccaa agacgctgaa gtcgttctgg tggaaggtct
     7921 ggtcccgaca cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ccaaaacgct
     7981 gaacgcagaa atcgtcttcg ttatgtctca gggcactgat actccggaac agttgaaaga
     8041 gcgtatcgaa ctgactcgca acagcttcgg cggtgcaaaa aacaccaata ttaccggcgt
     8101 tatcgttaac aaactgaacg ctccggttga tgagcagggt cgtacccgtc cggatctgtc
     8161 cgagattttt gacgactcca ccaaagcaaa agtgaacaac gttgatccgg cgaagctgca
     8221 agaatccagc ccgctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac
     8281 tcgtgcgatc gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa
     8341 tactcgccgc gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca
     8401 cttccgtgcc ggttctctgc tggtgacttc cgcagaccgc cctgacgtgc tggttgccgc
     8461 ttgcctggct gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga
     8521 aatggacgcg cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt
     8581 tatggtgaac accaacacct ggcagacttc tcttagcctg cagagcttca acctggaagt
     8641 tccggttgac gatcatgagc gtatcgaaaa agttcaggaa tacgttgcta actacatcaa
     8701 cgctgactgg atcgattctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc
     8761 agcgttccgt tatcagctga ctgaacttgc gcgcaaagcg ggcaaacgta tcgttctgcc
     8821 ggaaggtgac gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc
     8881 aacttgcgta ctgctgggta atccagcaga gatcaaccgt gttgcagcct ctcagggtgt
     8941 agaactgggt gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa actatgttgg
     9001 tcgtctggtc gaactgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct
     9061 ggaagacaac gtggttctcg gtacgctgat gctggaacaa gatgaagttg atggtctggt
     9121 ttccggtgct gttcacacca ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac
     9181 tgcaccgggc agctccctgg tatcttccgt gttcttcatg ctgttgccgg aacaggttta
     9241 cgtttacggt gactgtgcga tcaacccgga tccgaccgca gaacagctgg cagaaatcgc
     9301 gattcagtcc gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc
     9361 ctactccacc ggtacttctg gtgctggtag cgacgtagaa aaagttcgcg aagcaactcg
     9421 tctggcgcag gaaaaacgtc ctgatctgat gatcgacggt ccgctgcagt acgacgctgc
     9481 ggtaatggct gacgttgcga aatccaaagc accgaactct ccggttgcag gtcgcgctac
     9541 cgtgttcatc ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc
     9601 tgctgacctg atctctatcg gaccgatgct gcagggtatg cgcaagccgg ttaacgacct
     9661 gtcccgtggc gcactggttg atgatatcgt ctacaccatc gcgctgactg cgattcagtc
     9721 tgcacagcag cagtaatctc gtcatcatcc gcagctttgc gctgcggata tctgaaccgg
     9781 aaatagtcac tatttccggt tttttattct attaatttgc attaatcctt tctgattatc
     9841 ttgcttaact gcgctgcatc aatgaattgc gccatctcac tttgcatact taccactttg
     9901 ttttgtgcaa gggaatattt gcgctatgtc cgcaatcact gaatctaaac caacaagaag
     9961 atgggcaatg cccgatacgt tggtgattat cttttttgtt gccattttaa ccagccttgc
    10021 cacctgggta gttccggtgg gcatgtttga cagtcaggaa gtgcagtatc aggttgatgg
    10081 tcaaacaaaa acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc
    10141 aggcgaacct gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggtct
    10201 gatgaacttc ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat
    10261 catcatgttt atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat
    10321 tgataacggt atcctggcgc ttattcgcca tacccgcgga aatgaaattc tctttattcc
    10381 tgcgctgttt attctgtttt cacttggcgg cgcaatattt ggtatgggag aagaggccgt
    10441 cgcctttgcc attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac
    10501 cgtccttgtg acctatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt
    10561 ttgtgtggtt gttgctcagg gtattgccgg cgttccggtg ctttctggct ccgggttgcg
    10621 catcgtggtg tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc
    10681 acgagtgaaa aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga
    10741 aaagcaggct gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt
    10801 cctgaccgcc gtaatggtct gggttatttg gggcgtgatc gttaatgcct ggtttattcc
    10861 tgaaattgcc agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt
    10921 ccgccttaac ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat
    10981 gatgatcgcc cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggtcggtaa
    11041 tggtgaagcg ggtgatgcca gcgtgttaaa taccatcctc aacagcattg ccaatgccat
    11101 tagcggtctg gataacgcag tcgcggcctg gtttatgttg ctcttccagg cggtatttaa
    11161 tttcttcgtg acgtccggtt ctggtcaggc ggcgttaacc atgccgttac tggcaccgct
    11221 tggcgatctg gtcggtgtta accgtcaggt taccgtgctg gcgttccagt ttggtgatgg
    11281 cttcagccac atcatttacc caacctcagc ttcgttaatg gcgacgctcg gtgtttgccg
    11341 ggtggacttc cgtaactggc tgaaggtggg tgcgaccctg cttggactgc tgtttattat
    11401 gtccagcgtc gtggtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc
    11461 gcgattgcgg ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt
    11521 gcgcttcatc cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc
    11581 ggtgatttct tccggcgtca gccagcaaac ttcactgact tcatcttcct gtagagcgaa
    11641 gggaccgtga gagacgcagc tgaacaacgc gccccagaca cggcaatttt tatcttcgaa
    11701 atagaactgc ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc
    11761 gcgacgcgcg gattccagca gctgctcatc ggcctggact acaccgcctg cggtcgcatc
    11821 taacatgccg ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat
    11881 gccatcatgc acgacgatat aagttgcacg atgacgcaga cactgtgccc gcatttgttc
    11941 ccggctggct tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact
    12001 tgccaaacga cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca
    12061 ttttgagttg tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt
    12121 gagtgcgatt acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc
    12181 tttcggaata ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac
    12241 tggcagatgg gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc
    12301 cgggccaaaa agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg
    12361 ccacggcgcg gttatcggga aatgcagcag catttgatcc acttcgctgt cgcagttgcc
    12421 gcgcacagcg ataaccttat gtgccacttc attaagccgt tcagcgactt tggctggcgc
    12481 gtaaccctcc ggtaaagcat tacgcgggcc atgattcaac acgtcgccaa ggatcaccag
    12541 ccactgggca ccgctttgga caaacaactc cagaacacgt tccgtcgccg gtaacgaccc
    12601 atgaatgtcc gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct
    12661 atgatactgg attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac
    12721 gctggacaga cgctcgctgc cactggaatg tcgcataatc caccaggcgt tccggcacct
    12781 catcgccatg tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc
    12841 attcaccaaa taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt
    12901 tctctgcact ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa
    12961 catccgtcgg gcgttcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatct
    13021 gtcgtgctcg cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg
    13081 caaatcgatc ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga
    13141 tttgtaataa cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt
    13201 caccgctgtc gaggtcgatg gtcttgatat gaaacgacag gcctttctcc tgcaacgcca
    13261 cccaggcgga taacacataa ggggagaaaa aatgggcatc tgaccaaagc gtgatagcgg
    13321 gtttactcat tatgtcctcg gcaataaagg gggcttgcta tcaaactata gagccttttt
    13381 gtcactgtca cgtgacgaat actcacgcaa gtcgccgcgc tttctatact tgtggtgttc
    13441 agatgattag acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg
    13501 tcacaaaatt acgctgtttc tcgaagaagc agggctggat tatcgcttaa taaaggtaga
    13561 cctggggaaa ggaggtcagt ttcgcccgga atttttgcgc atttcgccta acaacaaaat
    13621 tccggcaatt gttgatcatt ctccagccga tggcggcgaa ccgctaagcc tctttgagtc
    13681 tggtgccatt ttgttgtatc tggctgagaa aacgggactc tttttgagtc atgaaacgcg
    13741 tgaacgcgcc gtcacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct
    13801 tgggcaaaat catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg
    13861 ttatcaggtt gaaactcagc gtctgtacca tgtactgaac aagcggctgg aaaattcgcc
    13921 ctggctggga ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc
    13981 ctggactcgc cagcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg
    14041 gatccgttca cgccctgcca ccgggctggc actgctaaaa gcacaacacg gtgatgagcg
    14101 ttcggatagt taacagaaac aggttctcgt gtattatttc attctaagta aaacaacgga
    14161 gaacctgcaa tggcacaacc tgccgctatt attcgtataa agaaccttcg tttacgtacg
    14221 tttatcggaa ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg
    14281 atccactacc ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat
    14341 cgcaccgtaa cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa
    14401 aaattaactc aggatgtgct cgatatcgca cgtgaacatc actgggtgac gtatgctgaa
    14461 gtggagatcg ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc
    14521 tggcagcgtt aaccgccata ttgggaggct gcatgaacat agtgatcacc ggagggacgg
    14581 gattaattgg tcgctatttg attccacgtt tgctggacct gggccatcaa atcacggtag
    14641 tgacgcgtaa cccgcagaaa gccagttccg ttctcggccc tcgggtgaca ctatggcaag
    14701 ggcttgccgg acaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac
    14761 cgattgctga taaacgctgg actcacgagc aaaaagagcg tctctgccaa agccgctgga
    14821 atatcacgca aaaactggtc gatttgatta atgccagcga cacgccaccg tcggtactca
    14881 tttccggctc ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg
    14941 aaccgccgca taacgaattt acccataaac tctgcgcccg ctgggaagaa attgcctgcc
    15001 gggcacaaag tgacaaaacg cgagtgtgcc tgctgcgtac cggcgtagtg ctggcaccgg
    15061 atggcggtat tctcggtaaa atgctgccgc cgtttcgtct tggcctgggc gggccgattg
    15121 gttccggtcg gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct
    15181 ggctgctgga taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca
    15241 atgaacaatt tgcccatgcg ctcggtcatg cgctgcatcg cccggccatt ttgcgcgtcc
    15301 ccgcgaccgc cattcgactg ttaatgggcg aatcttcagt actggtatta ggtggacaac
    15361 gcgcgctgcc taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag
    15421 aggcgctggc ggatgtcgtt cgctgatgtg gtttactgta aacatccgcc agcggaaacg
    15481 ctgatgaata aatatagata attcagaact atttttagcg gactgcaact tttacatttc
    15541 agcggaagcg gactcgcaaa ttccccatga aatactccgg cagcgttaac caccggagag
    15601 gaaattattt cagcgaacct ttaaggaact gttgcagacg agggctttgc gggttaccga
    15661 ataactgctc cggcgcccct tcttcttcta ttttcccctg atggaggaaa atgacgtgag
    15721 tagaaacatg acgagcaaag cccatttcgt gagtcactac caccatggtt ttcccctctt
    15781 ctgccagttg ctgcataata cgcaacactt cgcctaccag ttcaggatcg agcgctgaag
    15841 taggttcatc aaacagtaaa acttccggtt ccatcgctag cgcccgcgcg atagaaacac
    15901 gctgttgctg accaccggac aggtgtaccg ggtatttccc ctgcgcacgt tcgtctatcc
    15961 cgacttttgc cagatatttc accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca
    16021 cctgaatcgg cgcttccatg acgttttcca gcaccgtcat atggctccag agattgaagt
    16081 gctggaatac catcgtcagg cgtgtgcgca ataagcgcag ctgattttta tcggcgactt
    16141 tgagttgacc gtctttatcg cgcaccagat tgatcgtctg gccattgacc acgatcgatc
    16201 cttcgctcgg tttttcgagg aagttaatgc agcgcagaaa ggtactttta cccgatcccg
    16261 acgatccgat gatgcttatg acatctccgg cattcgcttg caatgagacc cctttcagca
    16321 cttcgtgttc gccgtagcgt ttgtgcaaat cgataacgtt taatttattc tcggacatcg
    16381 tattctcagt gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc agctctacga
    16441 aagaggctga tcaggacata agagatgatt aaatagagca ccgcggcaat gccgaaagcg
    16501 gtaaaaggtt gatatgtggc ggcgttaata tcgcgggcaa ttttcagcag atccggcacc
    16561 gtggcagtaa atgccagcgc agtagagtgc agcatcagga tcacttcgtt gctgtacgcc
    16621 ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcagc gatacatttt aaaggtcgag
    16681 aagccatagg ctctggcggc ttcaatttcc ccatgcggca ctgaacggat tgccccggca
    16741 aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt acagttcaga
    16801 ccactgcgaa aaaaggcgtt aaggaactcg gttcccttaa caatctccag cgtgtacatg
    16861 ccggaataga acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat
    16921 aaccagattg gaaactggat gtatttatta cttgagacgc gaccaatcgc cagaaacagc
    16981 gccagaactc cgcctatcac taccgacaaa atcagcagcc acagggtgat cgccacgcca
    17041 gtaaagcgat aaccgtcagt ccacagcagt ggtttccagt attcatgtaa gatttcgatc
    17101 acaggtcagc cctcttcaca cccacggagt agcggcgctc aaggaacagc agcacaccat
    17161 tggaaacggt ggtgaaaaca agataaatca cgccacagac gatagcgaaa tagaacggtt
    17221 cccaggtact tttgcctgcc agttgcgtgg ctttgaccac atcttccagg ccgagtaacg
    17281 aaaccagtgc ggtagatttg aggatcacct gccagttgtt gccaatgcct ggcagggcgt
    17341 aacgcatcat cgccggaaac atgatccgcc gaaacacttg cccacgagta aaaccgaacg
    17401 ccgtcgccgc ctctatatgt cctttcggca ctgccataaa cgctccacga aacgtttcgg
    17461 taaaataagc accgtagata aaaccgagag tgataatacc tgcgaccatc ggatcaatat
    17521 caatctgccc gacgcccatc gcctccgtca ccgtgtttag cgcaatctgc aaaccgtaga
    17581 aaatcagcag catcaatact aaatccggca cgcctcgaat cagcgtggtg tacccttcga
    17641 aaataaggcc cgaaagccga ttttgtgaga gcttaccgcc agcgccaatt aaaccgatga
    17701 ttacagcgag cactacagag ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa
    17761 taacacctga aaacccatac aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc
    17821 tcttgtctcc ctccggatac ccgaagggag cgggctttct ttcaccgatg gctattaacc
    17881 accataaaca tcaaaatcga agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg
    17941 catttcggca aaggctttgt tcagtgcctc gcgcagttcg ttatcttctt tacgcaggcc
    18001 catgccggtc cctacgccaa acagtttttc atctttaaca gacgggccac cgaatttgta
    18061 atctttaccg acgggttgtt tgaggaaacc ttcgctggct gcgacctcat cctggaacgc
    18121 ggcatcaata cgtccggcag tcaggtcaga ataaatattg tcctgcccct gataagaaac
    18181 gatttcaatg ccttttggtg cccagtgttc gttaccgaac gtctcctggg tggtgccctg
    18241 caatacgcct atccgtttgc ctttcagcga ttcgactgtc ggctgaatgt cggaattttt
    18301 cgccaccacc aaacgagaat cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg
    18361 acgtttttcc gtaatggaaa gcgatgacat gatggcgtca atcttcttcg cttttaagga
    18421 cgggattaac gcatcgagtg ggttttcgac aaacgtacat tgcgtattga tgcgtttgca
    18481 caactccttc gccagatcga tatcgaagcc aaccagttca ccttgtgaat tttttgattc
    18541 aaatggcgca taggtcggat cggtaccgat gcggatgttt tgcggaatcg cagcaaatgc
    18601 cgcagttgcg ctggagaagg ccagaaccag agagagcgat agcaccagtt ttttcataat
    18661 gtcctcaact gacagtcttt ttatcgggaa tatttacagg tttgttacac ttatcgtgcc
    18721 ataaaattga ccatcaaggc aaaatatccc gccccgctag acgtattatt cttcattaaa
    18781 aatgcttaag tatgcatttc aatgcaccac gatagtgcaa caaacctggc gtgcaccatg
    18841 atgatcattc catcaggtac agcttccctg cgacgtatca gtcaccgtag acattaaagt
    18901 cgaaatactt tttcgccatc ttgtcgtagg taccgtcctg acgcagctcg ccaagcgcct
    18961 tattaaacgc agccgtcagt tcagcatcat ctttacgcag ccctacaccg gtgccgtcac
    19021 cgaagtattt tttgtctttc actgatgggc ctgcaaaggc gaaatcttta ccggcgggtt
    19081 gcttaaggaa accttcgctg gcagcaactt catcttgtaa cgcagcatcc agacgtcctg
    19141 cagccagatc ggaatagact aaatcctggt tggcataggc caccacatcc acgcctttgc
    19201 tacgccaggt ctcgttagcg tatgcttcct gggttgatcc ctgcagcaca ccaacatgtt
    19261 tacctttcag tgaatccagc gtaggctgaa tcggtgaacc tttggcggca atcaaacgag
    19321 aatctgcggc gtacagcttg tcagagaagg ctatctcctg ctgacgttta tcggtaatgg
    19381 agagcgacga aataatggcg tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa
    19441 agtcactggc aacccaggta catttcacct gcatccgttt gcacatctcg ttaccgagat
    19501 cgatatcaaa gccaacaaag tcacctttgg catctttcga tgagaacggt gcgtaggtgg
    19561 tatcggttcc gatacgtacc gtctccggta gcgccgcata gctggaagcc gctgcggaga
    19621 gaccgactaa caaagacaga gcgagaatcg acttcttcat acataaccct caagtgaaaa
    19681 tggctttttt atgttgtgta ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat
    19741 gccatcttga cgacaacagt aacattcaac gttaaatatg ttaataagac gttgcattat
    19801 tgtcctgaag ttgaagatag caggtatagc ggttgaatcg cagcgtttcg attgaatggc
    19861 agaaacaaaa tgtcgaggat ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag
    19921 cggagttatg cgccctgcca gcgggcaaag agatcttcag gaagggttat cgcaaactgg
    19981 tcaagaacac gattaaccgt ctgatttatc acatcatcaa gggattgtgg gcgatgataa
    20041 aacgccggaa cgggaggcat aatcaccgca ccgatttctg ccgcctgagt cattaaacgc
    20101 agatggccta agtgcaatgg tgtttcacgc acgcagagca ccaacgggcg acgctctttc
    20161 agcacaacat ctgccgcacg ggtcagtaag ccatcagtat agctatggac aatgccggaa
    20221 agggttttga ttgaacaggg taaaatcacc atccccagcg tctggaaaga accggaagag
    20281 atgctggcgg caatatcgcg cgcatcgtgc gtgacatcgg ctaacgcctg cacttcgcgc
    20341 agggaaaaat ccgtttcgag ggataaggtc tggcgcgccg cctggctcat taccagatgc
    20401 gtttcgatat ctgtgacatc gcgcagaacc tgtaataagc gcacgccata aatcgcgccg
    20461 ctggcaccgc tgatgcccac aataagtcgt ttcataaata gttgccctgt cagacttgca
    20521 ggcagacttt gcaggatttc ggcgggagtt gcaagtcagg gtgccagacc ggcaccctga
    20581 gcgaaggcat catccttcgt tatgcatttc gagattttcc acttcgttct gacgttgcac
    20641 tgctttggcg tcgtcattac gtaaagtatc gaggaaatcg aggtagccct gatcaacatc
    20701 tttggtgacg tagacgccgt tgaacaccga gcattcaaac tgctggatat ccgggttttc
    20761 agcgcgaacg gcttcgatca gatcgttcag atcctggaaa atcaacccgt cagcaccgat
    20821 gatctggcga atttcatcta cttcgcgacc gtgagcgatc agttccgtag cgctcggcat
    20881 atcgataccg taaacgttcg ggaagcgaat ttccggtgcc gccgaggcga ggtacacttt
    20941 cttcgcgccg gcttcgcgtg ccatctcgat aatctgctca gaagtggtgc cacggacgat
    21001 ggagtcgtcg accagcagga cgtttttatc gcggaactcg gcgcggttgg cgttcagttt
    21061 acggcgcacg gacttacgac gcagttgctg gcccggcatg atgaaggtgc ggccaacata
    21121 gcggttttta acgaagccct ggcggtacgg tttgcccaga atacgcgcaa tttccagcgc
    21181 gatatcacac gaggtttccg ggatcgggat caccacgtcg atatccagat cttcccattc
    21241 gcgggcaatt ttctcgccca gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat
    21301 tttgtcgatg aacgagtccg ggcgagcaaa gtatacatac tcaaacaggc agggattgct
    21361 gaccggattg tcagcacatt gacgggtaaa caactgccct tcttcagtga tgtaaatcgc
    21421 ttcgcccggc gcgacgtcac gcaggaaatc aaagcccagc gtatcgagcg caacgctttc
    21481 ggaagcgacc atatattctg tacggttctc gtcaatatca cgtttcccca gtaccagcgg
    21541 acgaatcccg tttggatcgc ggaaagcaac cataccgtgg ccgataatca tcgccacaca
    21601 ggcatacgcg ccgcggatta agcggtttgt agcagcgatg gcggcgaaaa tgttgtcggc
    21661 ttccagcggg tagtggcgga agttgtccag ttcgctggca aagatattaa gcagaatttc
    21721 cgagtcggaa gtggtgttga tgtggcggcg tttttcttca aacagttttt tacgcaactc
    21781 gtgagcgttg gtcagattgc cgttgtgggc aagcgtaatg ccatacggag agttaacgta
    21841 aaacggctgc gcttcagagg cgctggagct gccagccgta gggtaacgca catgaccaat
    21901 gcccatattg ccctgcaaac gctgcatatg gcgagcttca aatacatcgc tcaccagccc
    21961 gttcgcttta cgcaaacgga agcagttatt ggcatctatg gtgatgatgc cggcggcatc
    22021 ctgaccgcga tgctgaagca ccgttaaggc atcataaatc gactggttaa ccggcataac
    22081 accggcgata ccgacaatac cgcacatacg tctttttcct cgttaagcca catctcagag
    22141 cacttacgct ctgggcaaga aacttgacga gctttgcaga taatcaaaaa accatctgat
    22201 gataaaactg aactgcggga tcagctgtga tttgctccag tcttcgcttt tcgacacccc
    22261 ggtaaaggag tcgagaaaga agagaatggc agcaacaatc aacacaccgc gcaacgcacc
    22321 gaaacagacg cccaacaccc gatcggtgcc tgacaacccc gttttctcca ccaactggcc
    22381 tatcacgaag ttcacgatag caccaacgat cagggtagcg ataaacagta ccgcgatggc
    22441 aatcccattt cgaaccagtt cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta
    22501 gtaatgactg gcaacaaaga aagcacaacc ccatgtcacc agcgataacg cttcacgaac
    22561 aaagccgcgg atcaggctaa ccagagagga aaaagcaatc accgcgatta tggcgtaatc
    22621 aatccagacc atatgtgtcc cacgatttta cgccctgtca tcctgttcgg ggcgcattct
    22681 aacagaaaaa gaaaacgttt gcgtagggat ttccttcccg cgcatcaata aaaatggcgc
    22741 tgaaaaaata ttcaacgcca tcgacttttt atgcctttgc ggcatcgggc aatgcgtgtc
    22801 ggatgccgca tgaacgcctt atccgaccta cagttctgct cc
//
LOCUS       DADTFG010000078        22277 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.078, whole genome shotgun sequence.
ACCESSION   DADTFG010000078 DADTFG010000000
VERSION     DADTFG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.078"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(150..839)
                     /locus_tag="J1138_002744"
     CDS             complement(150..839)
                     /locus_tag="J1138_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBA8454516.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(950..1654)
                     /locus_tag="J1138_002745"
     CDS             complement(950..1654)
                     /locus_tag="J1138_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8454517.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(1641..2477)
                     /locus_tag="J1138_002746"
     CDS             complement(1641..2477)
                     /locus_tag="J1138_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8454518.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(2470..3303)
                     /locus_tag="J1138_002747"
     CDS             complement(2470..3303)
                     /locus_tag="J1138_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8454519.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(3303..4352)
                     /locus_tag="J1138_002748"
     CDS             complement(3303..4352)
                     /locus_tag="J1138_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8454520.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSVFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            complement(4452..6020)
                     /locus_tag="J1138_002749"
     CDS             complement(4452..6020)
                     /locus_tag="J1138_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA8454521.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKNVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPRALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSAKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            complement(6395..8542)
                     /gene="fdhF"
                     /locus_tag="J1138_002750"
     CDS             complement(6395..8542)
                     /gene="fdhF"
                     /locus_tag="J1138_002750"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8123..8125),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBA8454522.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVTSRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(8740..10206)
                     /gene="mdtP"
                     /locus_tag="J1138_002751"
     CDS             complement(8740..10206)
                     /gene="mdtP"
                     /locus_tag="J1138_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBA8454523.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDSLIQRTLSGSHPLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(10203..12254)
                     /gene="mdtO"
                     /locus_tag="J1138_002752"
     CDS             complement(10203..12254)
                     /gene="mdtO"
                     /locus_tag="J1138_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBA8454524.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEAVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHSITEGQCWQSDWRISESEAMTARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLHIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGV
                     ATALSHSPQITLEETSASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(12254..13285)
                     /gene="mdtN"
                     /locus_tag="J1138_002753"
     CDS             complement(12254..13285)
                     /gene="mdtN"
                     /locus_tag="J1138_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBA8454525.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(13304..13579)
                     /locus_tag="J1138_002754"
     CDS             complement(13304..13579)
                     /locus_tag="J1138_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454526.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(13788..15773)
                     /gene="yjcS"
                     /locus_tag="J1138_002755"
     CDS             complement(13788..15773)
                     /gene="yjcS"
                     /locus_tag="J1138_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBA8454527.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIIAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPRNTQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(16046..16975)
                     /gene="alsK"
                     /locus_tag="J1138_002756"
     CDS             complement(16046..16975)
                     /gene="alsK"
                     /locus_tag="J1138_002756"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HBA8454528.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLYCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQSRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            complement(16959..17654)
                     /gene="rpe"
                     /locus_tag="J1138_002757"
     CDS             complement(16959..17654)
                     /gene="rpe"
                     /locus_tag="J1138_002757"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA8454529.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(17665..18645)
                     /gene="alsC"
                     /locus_tag="J1138_002758"
     CDS             complement(17665..18645)
                     /gene="alsC"
                     /locus_tag="J1138_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HBA8454530.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(18624..20156)
                     /gene="alsA"
                     /locus_tag="J1138_002759"
     CDS             complement(18624..20156)
                     /gene="alsA"
                     /locus_tag="J1138_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HBA8454531.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(20283..21218)
                     /gene="alsB"
                     /locus_tag="J1138_002760"
     CDS             complement(20283..21218)
                     /gene="alsB"
                     /locus_tag="J1138_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HBA8454532.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATKAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(21277..22167)
                     /gene="alsR"
                     /locus_tag="J1138_002761"
                     /gene_synonym="rpiR"
     CDS             complement(21277..22167)
                     /gene="alsR"
                     /locus_tag="J1138_002761"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HBA8454533.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT         5375 a         5817 c         5627 g         5458 t
ORIGIN      
        1 catcaatgtc tgatgcgacg gttgccgcgt cttatcaggc ctacgccaga cagcgcaata
       61 gcctgatttg gcgtgatttt tgtaggtcgg ataaggcgtt tacgccgcat ccgacatcaa
      121 tgcctgatgc gacgtttgct gcgcctacct taaccgttag ttaatttttc aaactcttca
      181 caaccggtat caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgtttg
      241 ttcttctcga taaaaccctc ttcaccgttt aagaacatca ttcccgccca gtattcggaa
      301 taaccggttc gggaaatcgc agagctgcgt ttgaaatacc aggttgcttt gtcatcgtcg
      361 gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca
      421 gagtcgttct ccagatcttc cgaggcgttt tctaacagcg aaatcgcctt tggataatcc
      481 ggtttacccg cttgcgtatt taccagaata tgcgccagcg ttacttcacc ttctttactg
      541 cccgcttgcg ctgctttctc ggcaagcact tttgcttgtg gatagtccag actgaccgga
      601 ttggtgattt tgatctgcgc caacagcgcg caggcatcgg catcaccgct gtcggcggct
      661 ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga
      721 aaatattgtg cgcgtcggtc tccggcctct gctgccttta aatactggct gccaggctcg
      781 gagtcgttcg cgtgggcaaa gaatgtcaaa aacaacatca aagtgataat ttttttcatt
      841 ttgataatga agtttggtag gggagagaca gtataaagtg ttcgggtgga caaaaaaaca
      901 actgtaaaaa gacctgaaag cagaatgaat taatgcaatg tcaggttttt taatccatcc
      961 gatgttcccc cttttccatg gcgtcgcgat caaaaaatcg ctgaattttt ccctcggaca
     1021 tcaatgccgc tcgatcagac atatgcgcga tgacatcggc atcgtgactg accagcagat
     1081 atgtcatttg gtgttccagc ttaagttgat tcaacaaatt aaggatttcc gcttgtaccg
     1141 acatatccaa cgccgaggtg ggttcatcca gcagaagaag ttgtggtcgt aacaacaggg
     1201 cgcgagcaat aaccacacgt tgccgttgac caccggaaag ctggtgtggg tagcgacgag
     1261 cggcgtcgaa aggcaagcca acctgttcca gtgcagtctg gacgcgtttt tctatttctc
     1321 tttcgccacg aattttgagt ggttcagcca gcgttcgcca cagagtatga ttgggatgca
     1381 acgaggcata gggatcctga aacaccattt gcacattgcg gcggagatcg ccctgaaaac
     1441 gcattcctgg tttgatggtg tgcccaaaca gtgacacctg accgtgccag tcgcgctgta
     1501 atccagcaat tacgcgcaaa atcgtggatt ttccgcagcc tgattcgcca attaaactaa
     1561 atgtctctcc ggcattaacc gaaaaactgg cggcagaaac tgcggttttc tcagcaaaag
     1621 tcacctgtaa gtgattaatt tcaacaatcg tcatggtatt tctccggcgt cattgccgtc
     1681 ctgtccagcg tcggcagcat ctgcccgtaa gtttgtgcat ttgggcggca agtccacagt
     1741 gtgcgcgtgt aaggatgcgt tgctgtaggt agcgcagcag cgtgcatttc atccacctta
     1801 tttccctgat acatcaccag cacccgatgg caaaattggg caactaatgg caggtcgtga
     1861 ctgatcagca acattgccat ctgacgttgt tcacactggg taaccagcag ttccagaatc
     1921 tgattacgca ggcgcacatc cagtgctgat gttggctcat cggcaatcaa tacccgggga
     1981 tcgttgatga gcgcaattgc aatcatcact cgctgcccca tgccgccaga aagttcacga
     2041 ggatagcttt gcagtactcg cgtattgagg ccgacagcct gaattgcggt gtttattttc
     2101 tcttctttct cgcggcggtt cagacgttga tggagcgtaa gcgcctcttc cagttgcgct
     2161 tttactgtct ttactggatt aagcgcgtaa cgcggatctt gcaataccat cgcgatatcg
     2221 ttaccgcgca actgttgcca tccacgcgcg ttaagggtga gtgcatcacg ccccagaatg
     2281 ttaagtgtgt ccgcggttac tacacccggt ttacgcacca gccccatcag ggcgcgggcg
     2341 gtcatggact tacctgaacc cgactcaccg accagcgcca gacgttcatt gcctaatgta
     2401 aaactgacat tgttgacgac ccgggactcc ggataatcga cgcagagatt gttgatacta
     2461 agacgtgagt cagtcatgtt gaggctccag aacatcgcgc aagccatcac ccagcagatt
     2521 aaaggccaga ctggcaatta agataattgc gccgggagcg gcggcaatcc accattgatc
     2581 aaaaatgacc tgcataccgt cggcgatcat ggctccccat tccgccattg gcgggcgagc
     2641 accaagccca agaaaaccca ggccagcagc cgccagaatg atacccgcta aatccagcgc
     2701 cagacggaca atggcagatg gcaggcacaa tggtaaaata tgaccagcca gcagacgcct
     2761 gccgcggatg cccatcattt ctgcggcggc aagataatca ctgtgtcgca gacgttggat
     2821 ctcactccgt gcctgacgcg cataagcagg ccaggtggtt aatgccaggg ccagtgcgcc
     2881 gttaaccagc cccggcccca gcatggcaac aaaagcaaaa gccagaatta aacgcggcat
     2941 tgacattact acgtcagtaa aacgcatcag cacgcgttcc agccagccgc cgtagtaccc
     3001 ggctaaaatg ccaatcagca gaccgaccgg gagtgtaatg attgtcacca atgccaccag
     3061 ccccagtgcc gggcggctgc cgtagattaa acgtgaaagt aggtctcgcc cgtaactgtc
     3121 agtgccgagc cagtgctgac gactcggtgc ttgtaaccgc gccgccgcat cctgccagtt
     3181 aggatcgtga ggtgccagcc acggcgcgaa gagcgcaatt agcatcaata acaggataat
     3241 tatcaacccg caaaacgcgg cgggtgaatg gcgtaaacgg cgtaaaaaga gaaacatcgg
     3301 catcagcgta acctcggatc ggtcagccgg accagcaggt cggttaagtt atttatcaac
     3361 acaaagcaga cgccaatcag cagcgttcct cccatgattg ctgtggtatc acccgcgaag
     3421 agcgctgtag tgaggtagcg cccaatacct ggccatgaaa agacggtttc tgtcagtacc
     3481 gcgccttcca gcattgatgt ataggcgagg gcgataacgg tgagcaaggt gccgcgaatg
     3541 ttgggtaaaa catgacgtag caaaattgtc atctcgctgg cccctttggc gcgcgccagc
     3601 agaatatatt ctttattcat ttcgccaaga caggcggaac gagtcaggcg cgtaatactg
     3661 gcgagcgaga agtacgccag taataaaacc ggaagtatca aatggttgat tgcattggta
     3721 aatgcctcgc gatcgccaga aagccacgta tcaatcagcg caaatccgct gcggggttct
     3781 atggtgaatt gataaatatc atccagcctg cccggcccgg cactccattg cagtctggcg
     3841 tagaacagcg ccagcatcag cagaccaagc caaaaaacag ggacggaatt tcccagtaga
     3901 gttaatgttc gcacggtgaa atccagcgga gaaccgacat aacgggcgca aagtacaccc
     3961 gcaatgacgc cgacaacagc accgacaata agcgccaacg ttgccagttc aatggtcgcc
     4021 gggaatactg atagcaagtc ctggagtacc ggttgcccgg tggagctggc gatgcccaga
     4081 tcgccgtgtg ccagattcat caggtaatgc cagaactgga cggctaacgg ctgatccaat
     4141 cctaactggt ggcgtacctg atcataggtg gactggctgg cgtgatcgcc aacgatttgt
     4201 agtacgcgat cgaccggtga gagagccgaa agcgcaaagg taatcagcag caggccgagt
     4261 agcgtcagag caacggtaaa cagcccttgc aacagcgcga gcagagggcg cttgcctcgc
     4321 catctcgcgc gagtggaaaa aacgacagac atgatctttc catacagtga taaatcagcg
     4381 gctggcctgc aatacaggcc agccctggta ccgcgcccgg taacagggct ggacgcgagt
     4441 tgcatacagg gttatttggt tacctgatca aaccagacca tatcggcatt caacccttgt
     4501 tgatacccct gcacattatc acgcatgaca atttgcgttt tgccttgatc aataaagaca
     4561 tacggggaac tacgttgtag ttcttgttgc attttcttat acaaatcaag acgtttagcg
     4621 ctatcaggtt cagcgatggc cgccagcgtt tctttgttca gttccgggat tttccagccg
     4681 tttaacccgg caacggtgct ggatttgcca tcgttatagg cgaaagagct ggcgttggaa
     4741 tgtgcgtcaa aataatcagg caaccacata cgaatggccc cctgatgctg gtgggcgcga
     4801 acgcgagagt agacctgact ccctgcggct ggcagcaaat caacttttac accgccctga
     4861 gcaaagctgg cctgaattga ctgcgcgatg gtaataaatg gcggtttatt ttcgacatca
     4921 agcgtgaagt gcgggtcttt gattccggct ttagcgagga tctcttttgc tttggctgga
     4981 ttaaaggtaa acggtgtttc ttccagtgct ctaggaaagc ccacgggcag gaaactttga
     5041 tgggtaaaat attgtccttt taagagattt ttggtgatgc cgtcgtaatc gaccaaccag
     5101 cgagcggcct cccagaacgc aggattactc atcagcgggt tatccttgtt accggtattg
     5161 aaggccaggt agttctgttc ggcggaggca atgctcatca cttttacgcc aggtttaccc
     5221 tgtaatgcat cgatttgatc ggctcccaga tcacgagcca tatcagcatc accttgttgg
     5281 atcagcaggc gacgcgttgc cggatcggga acatttttga tgatgacgtt tttcagtttc
     5341 ggggcgccgg ttggtgagct ggcgttggct tccatcacga tggcctgatg cggttggtaa
     5401 gtacgcattt taaacgcgcc gctgccagcg gaatgcatct tcagccattc attaccaaag
     5461 tcgttatttt tggcgtttgg cgcaaccagc ttctcgtcaa caattgaggc gattggtgtg
     5521 gagagaatat tcagtgccag cgccgggctg acgtcggcac tccattgtac ctggacggta
     5581 tggtcatcga ttttcttcaa ctggctggcg atattttccg gctgccatcc cagcacattg
     5641 agaataaacg caccggattt attcagtgtg acagcccgga cataagagta aatcacatct
     5701 tccgggcgta ccggattacc agaggcaaat ttcgcatcgc ttttcagttt tatggttagc
     5761 gttttggctg cggggtcagc ctgccaactt tccgccagaa tcggagtgat tttcgtcgga
     5821 tcattacggt ccggttgtac tacgcgttga tacagacttg gtacagtctg aatacttgag
     5881 agttcattag cttctgccgg gtccagactc acaatatcat ccagtccctg ggctacaacc
     5941 aacgtattcg gcggtgtagc ggcaacggtt gtggcggaaa ttgtgcttaa aaccagtaaa
     6001 ggtaatagtt ttttaatcat atgcttccca atccctgaaa ttactttgag gttattaata
     6061 tgctatcaga cgggggttcg ctgcattaat cgcgccaaaa tatcccgtta atagaacgag
     6121 ttaaacggtc gatgatttct caccttaccg aaattttgtt tatcaaatgt aaagtattat
     6181 tacgttaggc cgcgatgaga attgagtaaa gataattctc atttgcaatc gtgactggaa
     6241 tagaagaaca accgagaaga cgtcgctaat aaccaggatg ggctggtttg actgcgcagg
     6301 tatgtataaa aaaatccgga aacagagagt cgtttccgga ttgatacaaa ggcgttgggt
     6361 aatatcgccg cttttaacgt gagtttaagc ggtattacgc cagtgccgct tcgcgcaggc
     6421 gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc tgatcggcga
     6481 tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc gggcttaagt
     6541 tttcggtcac cagctcgtta caggcaccaa tccaccactg gtaggtcatg taaatcgccc
     6601 ctttgttcgg acgatcgctg acttgcgcac gggtgataat tttgccttta cgcgagtgca
     6661 cccaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta ttgatttgtg
     6721 cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccagtc atcgaacggc
     6781 aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtattcg tcggtgagtt
     6841 tgtcgattgg cgctacccag tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa
     6901 acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg cggcaaggcc
     6961 actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa tccgggcaca
     7021 gatgacgcaa ctcgtcccag atctcctggg tgttgttgta gtgcatcgga taacccatac
     7081 gagtggcaat ttcactgatg atttgccagt ccgttttcag atcccacttc ggctcaaccg
     7141 ccttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc
     7201 acgacgttga cggtaaaatg acatccgccg ccgacgcggt tttggtcata aagatgtcct
     7261 gaacgataac cagttccaga tcttcaaagg ctttacgtac tgccgacagt tccgcgtcag
     7321 tttgcagcgg atcttcgccc ataatgtacg ccgctctcac ttcaccatgc gccacgcggt
     7381 gcggcagctc gctgatgcga tagccggtat gagcaggcag gctttccacg ccccaggctt
     7441 tggcgaattt ctcgcggtta gccggatctt tcacgtactg atagcccgga tacgtatccg
     7501 gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc gggtttacac
     7561 ccgcatgtgg cttaccgagg ttaccggtca gcatcgcgag gctggtcaga gaacgcacgg
     7621 tttccacgcc ctgatagaac tgggttaccc ccatgcccca caggatggcg gcgctttccg
     7681 cctgggcata catccgcgcc gcctgacgaa tctcactggc gctgacgccg gtgatatctt
     7741 caaccgactc cggtgtgtag ccttcaacga ttttacgata ctcttcaaag ccttctgtac
     7801 gggaagtgac gaacgctttg tcgtacagat tttcttcaat aatgacatgg cccatcgcat
     7861 tcaacagcgc gatgttcgag ccgtttttca atgcaatgtg catgtcagca atgcgcgcgg
     7921 tttcaatttt acgcggatcg cagacgataa ttttcgcccc gttacgttta gcgttaatta
     7981 cgtgattcgc cacgattggg tgggaatccg ccgggttata cccgaaaacg aacactaaat
     8041 cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc gattggtgca
     8101 gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa
     8161 taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta ccgcgcgagg
     8221 agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc aggcgctcgg
     8281 caacgtaatt cagtgcctca tcccaggaaa caggttcgag tttgccgcca cgctggcgac
     8341 ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg aagtcccagc
     8401 cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct gcctccgccc
     8461 ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg caataggggc
     8521 aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg catacgcgct
     8581 ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc ctgatattac
     8641 gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga
     8701 aacgaaatac cgcgtgacag ccatcacgcg gtagacattt tattttttct cgacgacggg
     8761 agccgcctga tacccgccgc ccagcgattt catcaactga atgctttgga tcacccggcg
     8821 gctgtccagc atcagtaatg acatctcctc ggcaagcact ggcaaccggg cttcggtggc
     8881 ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gctgcgcgct gggtgaagcg
     8941 cgttgcttct acgcgttcag cctgcatctc tcgctcgtcg ttgagcgttt gcagacgcgt
     9001 gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac gttcaatcat
     9061 catgttgctg gcggcgcgag tgccttcgag attggcattc aaccgtccac cgtcaaacag
     9121 cggcaatttc agacccggga tgaagttgaa ctggcgactg gtttttttga ataaggtatc
     9181 caggtggatg gagtccagac cgaaaaacgc tttgatatca aagctcgggt agaacagcgc
     9241 ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg cttgcagatc
     9301 cgggcgtctg gcgagcaact cataagagag cgttgccgga atgccggtct gtactcgtgg
     9361 taatgccacc ggtttgatct ccggcatatc gctggctccc gcgccaatca atgcacgcag
     9421 agattctcgc gtttcagtga tttgcccttt gacggcagca atttgtttat cgaccgccag
     9481 aatctgtgcc cgcgcgccgt ggaaaggcac ttgcgcttcc agaccgtgcg ccactttact
     9541 ctggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca gatcgagcat
     9601 ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca gcgatagctc
     9661 tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcag caaccgctga
     9721 gcgatgcaca ccccacaaat cgagatccag tccggcaaac aaaccgactg tggcttccgt
     9781 atagtacggc ccgtccatac ccagtgctgg cgcatccatc gcataagggc ttaaaaagcc
     9841 gttcgccgag acgcgttggc ggttgagcat ccctaacgct gcgacctgta attgtgaacc
     9901 ggcatctaac aaatcggcct gggactgcgc tttttcttcc cgcagtttcg cttcggcgag
     9961 ggggtgtgaa ccacttagcg tccgttggat cagcgaatcc agctgcgggt cattgagttg
    10021 tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcat cggccagttt
    10081 gatttgctcc ggtttgagct gttgatgagg cgcagaatcc ttacgtacca gggcacagcc
    10141 ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa gttgacgatt
    10201 gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg ggctgtccag
    10261 tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa ggtgggcaga
    10321 atggcctgcg aggcggatgt ctcttctaac gttatttgag gagaatgact gagtgcggta
    10381 gccacaccgg cagcgtattt ctccagggcg tcggcaaact gacctgctct gtccggctgt
    10441 agtgcgttat ccagcgcctg cgccgagttc caggtggcat cccaggcgtg aagaatatcg
    10501 cggccctgac gaataaccgt ttgcgaacgt tcaatcagta atgcgcgttc ttcgctgtcc
    10561 agttgacgct ccagcaccac gcgttggcac atctcttcac aggcattaaa cgccgcatgc
    10621 agacctatgc gaatgtgcag ataagtgcgc agagccgtga cttcctgctg gcgtgggatc
    10681 cgcattactt tactcaacat acccagcgcg ccagccagtt tttgcggcag cgtgcgcgct
    10741 tcgctttcag gccagacaaa ggtgtaaatc accgccgaca ccacggtacc aatgatgata
    10801 cccagagcgc gatcgcgaat ttccaccagg tcgtacaccg ggccaaaaac gttttcgagc
    10861 gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg ttcagagctg
    10921 gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca caaacagcaa ttcgacaata
    10981 ttgtccagcc agggcatgac cagtagcgtg aataacagcg ccagaatcgc gccgcaaaag
    11041 gccccaccaa aacgcagcac catcttctgg tacgacgaac cgacatttgg attagcgacg
    11101 atcacgcagg tcagcataca ggtatgaatg ccttcccagt ccacgccgct gtagaaggtg
    11161 taacagatca aacaggcgag cagcgttttt accgcgtagc gcatatagtc tggattggta
    11221 aaagcatcgg cgaccattga tggcggtttg gctgcgggcg ttggcggcgt attcgggtcc
    11281 atctgaccaa gctgtaacag cgtctggcag atattctcca ggttacattc ccgtgccgtc
    11341 atcgcttcac tttcactgat ccgccagtcg ctttgccagc actgaccttc ggtaatggaa
    11401 tgctgcagct tgttgatttc tgaagctaat ttttgtcgga attcaataat tgcctgagaa
    11461 tcagcaaaag aggtgggatc gtagcgattc agtgtcgagt aaatgtaggt taccgttgcc
    11521 acgcagcttt gccaccatgc gctctgggtt cgccagttgg catcgtccgc gaggcaaaag
    11581 acattgagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg tagcggtgcg
    11641 aggctgtccg tcaggtggct aatggcatca tcaagccgat cattaagcgc ctgatgcatc
    11701 tgcgtaatgg cacgactggg aaaccacagc acgccgatta acgtcatcag caaggttgga
    11761 tagaggccaa caacgataca ccacagcgtt aagcgcacga ccgcttccgg atagtcgagc
    11821 atagcgggga aggtttgccc gtaaatagcg acaatggcga cggcgaaaaa gaccagtccc
    11881 agccgatggg tgcgcatcaa aaacatgcag cccatcagga tcggcccggc gatgatcaat
    11941 cggatcaacg gttcgccata tgaccatttg tagatcaaaa acaggctgcc gatctccagc
    12001 accgtggcaa ccacaaacaa gatcgcgaca aatttggtgt aaaacgcgtt cgactgaata
    12061 ccgtaaaaca gcactgccag tgataacgcc acaaaaggga tctcaaaagt catcgagatc
    12121 agaatcacca gcaggcaacc tacccagagt tgcatcgtct gtggcacgcg ccctggtcgc
    12181 cgctcgctta actcttcatg aaagaacgcc agcagcctga ccaccggtaa tggcagggag
    12241 ttgagcgcgc tcattattgc ggctcaagat tagcgactgc cgaagcgccg atgcggaaca
    12301 tttccgggtc aggtttatcg accatgattt tgaccggaaa acgctgggca acgcggaccc
    12361 agttaataga acgagacact ttcggcaggc cgcccagcac caggccgccg tcatccggta
    12421 gcacgccgta gccaatcgaa tccactttac cctcgaaggt tttgccgctg tcgctcatca
    12481 ggcgaatggt tgcgggtgta cccgagcgaa tatttttcag atcagtttcg cggaagttgg
    12541 caatcacgta ccagtgacga gtgtcgatta gagtgaaaat agggcgcatc gcagatgcaa
    12601 actgcccgac ggaggttttg agggaaatga cccggccatc aaacggcgcg cgaacggtcg
    12661 ccatttccag atgcagtttg gtcagggcaa tatccgcttc cactgccgca cgctgcgcca
    12721 ccagcgcatc cacgccgctg acggcgctgg cggctgactg cgcctgcaac aatacggcat
    12781 taaggtccgc ttctgccgca cgctgtgcgg ttcttgcacg gtcaacctct tccgcagaaa
    12841 caaaaccttc tcgcagtagc ggttcggtac ggcgtaacgt atcgctggct tgtttcgcgg
    12901 cggcacgggc tttttctacc gtggcattaa ccgagtcggc accaaactgt tgcgcgtcaa
    12961 cgctacgctg ggtgagcata atttgcttat ccagcgccgc gagggaggct tcagctttcg
    13021 ccagattggc ttcgtacggg cgcgggtcaa tgcggaacag caaatcgccc tgtttcaccg
    13081 cttggttgtc ggtgaccgcc agttcaacaa tgcgaccgct gacttccggc accacatcaa
    13141 tggtatctgc tgacgcgtaa gcgtcattag ttgatggcgc actgtctacg cgccagataa
    13201 cgaaaacaag ggcaaccagc gccaacgcaa ccactaacag agcagggaat ttactgcgag
    13261 gagctttttt cggcgtactt tccatgagcg cgtctcaaat agattagaaa aatgccagcc
    13321 agaacagcat ggcgtagaga gcaaaaaggg cggtataaat aattccgaca aatgccagat
    13381 tgatatttgt ccgctgaata attcgcctcg tgatgagcgt caaaataagg ctggcaatag
    13441 cgcagaaaaa ccagccggga taataagcgc cgatcaccgg gatcgccgga gataatgagc
    13501 atcccgatac catgaagacg ggaattatcc aggctgtctt tttgagttgc atcgccatgc
    13561 gagagagaac tgttggcata ttaaatacat cctgtctgct gcgaacaata tctgtagtga
    13621 atatcaatat ccattgaagg cgtcagtatg gctattttct tgatgaataa aaatagcttt
    13681 attcagccac cgcattcata acttatatat tttatgtatt aatttgctgt atatattttt
    13741 aaaataaatg cttttggggg cttcagcgcc cccaaaagcg agtgacatta atttggggtt
    13801 acgatattca cccacaaatc gaaattatcc agacaggcga taatttcttc cagtttcgcg
    13861 ccattgccaa taattttggc tttcttcgct ttcaccagat cggccatttt tgcctgtccg
    13921 gtcagcacgg cgtgcagatc ttcacggctg atgtagaaag aggcgtcagc ttgcggttgc
    13981 agtgttttac ggtagttaag cacgctatcg ttcagcgtca ggttgaggtt atcgccgttg
    14041 ctcatattga agttcaggct gatattttta cccgcggctt tcgcgctatc gaggcgaacg
    14101 gacataaagt cgaacagcat ttcgaccgac atcccacgaa tggtgtccgg ggaaccggtg
    14161 gtgccgtggc tgaacttatg caccccttcg cgcagctctt tcgcgccggt caggtagaaa
    14221 ccgcgccatg tggcggactc ggcctgatag cccagctgtt caaagttatt cgcttgcaga
    14281 ttcttcgcga cctggtcacc cgggttggcg gcaatcacct gtttcagcag ttctgccgac
    14341 cagcgataat caccttgctt gttcgcttct tgcgccaggt tgatgacgcg ggcagaaccg
    14401 cccagcgcct gcacgtaacg tttacccatc tccacctgac catacggatg caggttagcc
    14461 gggttaccgt cgtaatagcc aagatagaag ttatataccg cgcgggcgtt gtggctgaca
    14521 gaaccgtaat agccgcggct ggcccagtta ttggcaagcg caggcggcag cttaatcatg
    14581 tcgccgattt cattcatggt gtagccctgg ttcgccaggt gcagggtctg gtcgtgaatg
    14641 tacttgatag tatcgcgata tttaccaata taatcattga tatgcttatt gccccaaacc
    14701 ggccaggtgt gcggcataaa cagtacttcc gcgtcgttac cccacatatc caatgtttcg
    14761 ttcagatact cggtccactt gctggtgtcg cgggttttcg cgccgcgcag ggtgtagaag
    14821 ttgtgcaggg tatgcgtggc attctcggcg gtacacaggg ctttcagggc cggaatatag
    14881 aagtgcattt ccgctggcgc ttcgctacct ggggtcatca ggaagtcaaa ctccaggccg
    14941 tcgataatca tcttctcgcc agttctgacg atagttttag tcggtgcaat aatgctcggg
    15001 tcgcccgttg ccagcgtcac gccaaggcca ttacccacat tgccttgcgt gttacgcggc
    15061 agtaacagac cgtaagagta gagcgcacgg cggctcatga tgttacccgc cagcacgttt
    15121 tcgctgatgg cttcgtccat aaagccagct ggagcaatca cctgaacttt gccggattta
    15181 acatcggctt cagagataat acctttcacg ccgccatagt ggtcggcgtg gctgtgagtg
    15241 tagataacgg caataatggg tttttgcgga cgatgctgga agtaaaggtc aagtgcggct
    15301 tttgcggcag gtggcgtcac tagcgggtca ataacaataa tgcctttctc gccctcaacg
    15361 aacgtaatgt tagagatatc ctggccgcgc acctgataca ttttatcggt gactttgaac
    15421 aggcctgaaa tgccgttgat ctgcgactga cgccacaggc tggggtttac ggtttccggc
    15481 gcagcggcat taatatcaaa tttgtaatca ttcgcgcggt agtaaatttt accgttcgca
    15541 tcacgcagaa taccttcatc cagcagcgga gcgataaatc cacgctgagc atcgtcaaaa
    15601 tcctggcgat cgctaaacgg taatgatttg gcgtaattct gattaacctg ttgggtatat
    15661 tgagtggcag ctttcgcttc atctgttgcg taagcggtat ttatcatcat gccgctggcg
    15721 gcagtgaacg caataacaat cctgctcaaa cggaataacc gagagttatt catataaatc
    15781 tccatttatc atttatgtcg taaatatgta atccatgcaa aatacgtcat ttaaagctgg
    15841 cgttatcata attctgaaat tgcgacttta ataagtggaa gtgtgagcgg aacgatccat
    15901 tttattaggc atttgtgctg aagagggaaa attacagtag aactatcatt gctgggaata
    15961 atattggctg ttttatgtaa ttattatata tattgtacct gtcgttggca cagcgggaca
    16021 ttgcgctact gtgccaacgc tttactcatg ggactttagc acaggactgt ggcaaaaaac
    16081 gttgatgcgc caatattgct gcaccctgag cgccattaaa gtcagaagat gaggcggcaa
    16141 taaagcgcac gacctgatac ggcagtggac ggcgcaggta cttttgggtc atggcaatga
    16201 gagtctcgcg tgggaaggcg ggcatatcca tcacgccacc acccagaatc accgcatcgg
    16261 gatcgaacag attaatgctg gtggcaatgg ctcgtgccgc gttttcaagc agactctgga
    16321 cgaaaggggc gttttccgca tggacgaaaa gatcgctcaa tgggtaattt cgggactgtt
    16381 gttcgtacca gcgtcttagc gccattccag agcaattggt ttccaggcac ccagaattgc
    16441 cacacgcgca gtgttgggtc atatctccca gggggatatg gcccagttcg cctgccacac
    16501 cgtgtgcacc cgtccacggc gcaccgttca tccacactgc gaaccccatc cccgtaccga
    16561 gataggctgc cagaaccagt tgttgcgtaa ggcggttttc tactacgtcc caggaaagtt
    16621 gcaggttaac gtcgcgggaa aactcaaccg gacaattcag cgtattttcg agcttatcgg
    16681 cgagatcata taaatctgcc gctgttaacg gcaggttagg cgtagaaata atggtgcgtt
    16741 tatctttact gaccagcgcc ggaaatccca tcaccagacc atgacagcga gcgttaaagc
    16801 gcctgagttg ctcgtcaatc atttcgccga tacccgacac caggtcggga gcaataactt
    16861 ctgcggtccg ctttttttcg cagtatagcg tttcaccttc tgctgtccgc agacaaaagc
    16921 ggatatgcgt tgcccccata tccacgcccg ctacgacgtt atgctgtttt tgcatgaggc
    16981 tgtacctcgc tttttgcagc cagaatctgc gcagtcataa ttctccatgc ttcgtcgata
    17041 ttttccgcat gattaaacag gccggaagtg ccgacgataa agacatccgc ccctgccgcc
    17101 attagttttt cgtaagttgc ctggttgcag gaaccgtcca cctcaatttc gtactccaga
    17161 ccttctcgtt cacgccatgc cttcagttcg gcaactttat ccagcatttc aggaatgaag
    17221 ggttgtccgg caaaaccggg atcgacagtc atgaccgtaa ttttatcagc cttatggata
    17281 tagtatttca tggcctcaac cggcgtttcc ggattaagaa tcagccccac tttcatgcca
    17341 tgacggcgga tttcatcaat caggcggaat gcctgaccgt tgatagtttc cggatgcaaa
    17401 gtgatgaaat cagctcccgc acgtgccagt tgagcaatgt aatcctgcgg acgcgttacc
    17461 atcagatggc agtcgagcgg tttactcgcc agttttttta cctggcttac gaagaacggt
    17521 gagagcgtca gattcggcac aaagtggccg tccatgatat cgatgtgaaa gtaatcggca
    17581 tggctgtcga taaattcgat ctgttcttta aatttcagca gatccataca cattaacgag
    17641 ggggagattt tcatgttcaa ttccttactt actgataaga cggtcaaggg cgacagccgc
    17701 gataattaat ccgcccatca ccaccagttg gtaataggtt tgtacctgca aaatattcag
    17761 accgttgttg atggtgccga tgatcaaccc gccaatcacc acagagaaaa tgcgcccctt
    17821 gccgccgaag aaactggtgc cgccaatgat ggcgctggca atggcatagg tttcaaaacc
    17881 cataccggca agcggttctg cggcaccgag tcgtgcagtt gagacgacgc ctgccagacc
    17941 tgcacaaaca ccggagatga taaacaccac caggatgtgg aatttcacgt caatcccgga
    18001 atagaacgcc gagtttttgt tgccgcccag tgcgtagatg ttgcgcccga gccgcatacg
    18061 cgttgtcaga aaccaaagga tgagcgcgac aattagcgag aagataacgg ggacaggtat
    18121 cccaattacg ctggcggcaa agaagttcac gaagtcaaat gagaagccgt ataccgagtt
    18181 ggcatcggag atcaccagcg tgatcccacg gaaaatagcg ttggtgccaa gggtgataat
    18241 gaacgggtgt agtcccgtcc agttgaccag gcagccgttg atcgccccca gtgcgccgcc
    18301 aaccagtaca ccgccaatta gcgccgcgag aaacggatca atacctgcca acatcagttt
    18361 agcggtcacc ataccggaaa gcgccagaat cgcgccaacc gaaaggtcga taccggcgac
    18421 caggatagcg aagaattccc ccatgccgat cagtaccgtc acggagcttt gcacaaaaat
    18481 ctgggtaata ttattggtgg tcagaaaata ttctggcgac agcgaaccaa aaatggcgac
    18541 gatgatcgcc aggataaaaa aggtgccgta tttatcccag aacagcgcaa agttgaacgg
    18601 tttcttctcg ctcgcttcgc tttttactct tgtggtaaag cccatgccat aatctcctct
    18661 tcgctcatgt catcgcgatt ggtcaggatt tgcgtcagtc gtccttcgca gaacacggcg
    18721 atgcggtcgc agacggtgat aatttcaggt agttcagatg acaccatcag gatgactttt
    18781 ccgtcgtccg ccagttggcg catcactttg taaatttcgg ctttcgcgcc aacatcgatg
    18841 ccacgggtag gttcatcgaa aatgatcact tccgggcgac agcacagcca tttggagatc
    18901 aggactttct gctggttacc gccggagagt tcagtgatgt tctggtttac cgaatgacat
    18961 ttcagcgcca gcagttcgcg ttgattttca gcggtacgtt gctcgtcaac ttcatgaaac
    19021 aaccccatcg cgcctttata gccgccgtct ttcagactgc ggctgatagc tatgttctga
    19081 gcgatagaaa agttggggaa aaagccgtta tcccggcggc tttcagtgat gtaagccatc
    19141 ccttttttca cggcatccag gggagaacgt ggagagatat ctttgccatt aagacggatt
    19201 tctccgccag cgcgtttatc aacgccaaac aggcagttca tcagttcagt acgtccggaa
    19261 ccgaccagtc cggcaaagcc taatatttct ccccggcaga cgctaaatga gatatcccgg
    19321 accttttttc tgtcacggct ggtgacgttg cgcacctcaa aaaccgtatc gtgcgcaagg
    19381 ttgctgacat tctccttcat cgcgttaaaa cggttttgca attcgcggcc taccatcaga
    19441 cggacgatat cgtcatttga cacatcgctc accatgccgc tgcaaacgct gctgccgtct
    19501 ttcatcaccg tatagcggtc gcaaatacgg cgaatttccg ccaatttatg cgagatatag
    19561 acgatggccg taccctcctt acgcaactga ttcatgatca gaaacagata gtccacctct
    19621 ttattggtga gtgaggaggt gggttcatcc atgatgatga ctttggcatc gagcatcagc
    19681 gttttggcaa tttccagcat ctgcttgtgg ctgatagata agttcgccac tttctcatct
    19741 aaatcaactt tcaagcccac gcgtaataac atcatggcgg cgcggacacg catttctcgc
    19801 cagtcgataa tattgacgcc acagattttt ttcgtcagat gacgaccaat atataaattt
    19861 tccagtacgg ttaattcatc aataacgctg agttcctgat aaataatccc gataccgagt
    19921 tgtgccgcta atttatgatc cagtttgtta tagttaatgt tattaatggt aatggtgcct
    19981 ttggtcggct catgtatccc ggataacact ttcattagcg tagatttacc cgcaccattt
    20041 tctcctagta atgcatgtat ttcaccagga taaaccgtta aattaaccga ctttaatgcg
    20101 tgaaccggac caaaggactt gccgatcccc gccatcgata tatatggcgt ggccataaat
    20161 aacctctctg cacgaaatat cgtattattg cctgatggct acgtttatca ggtctacgtt
    20221 caggttattt tgttggccgg ataagacact tcattagcat catcatccgg catcattcag
    20281 ttttattgag tgaccaggat tgaatcgacc agtttaaatt ccggtgcttt atccagcggg
    20341 ataaccttgc cggatttctc agcgtcaacc atcagcttca gacccgttgc accgatatcc
    20401 gccgggttct gggcaaccgt cgcggtcatt tgtccggctt ccaccatttt gcgggcttcc
    20461 ggaatgccat ctgtaccgac gaccaacact ttgcccgttt ttccagcgtt tgcgactgcc
    20521 tgagcaacac ccattgccat cgtgtcattc gcgcaataga tcgctttaat attcggatta
    20581 cgttgcaata cgttagtggc gacatccagt gctttaatac ggtcccagtc ggcaggctgg
    20641 ctggcgacaa gcttgatctg gcttgctttt ttgaaggctt tggtggcacc attacgacgc
    20701 gcttcaccgg aggcgttacc ggctttaccc tcaatgattg cgacttcacc accttcagcg
    20761 cccaatttgt caataatgaa tgacgcgcct ttcgccccga cggcaacatt atcggtggtg
    20821 acaaaacctt ccacattacc gcctgctttt ttcagattat ccatgtcgat tttttcatcg
    20881 agattgacca gataaatgcc ttttttccat gcgcgggcaa caggcatgac caggttcact
    20941 gaggataatg gtgcgaaggc gatacctttg taatttttat tactgagatc ttcaaataac
    21001 tgcaattgag attgaaaatc gccttctgaa ggagaggcaa aaatatcaac gctgacgccc
    21061 agcgtttttg cttcatcttc aatgcctttt ttcatatcta cccaaaatgg attggagagt
    21121 gttttcaata cgacggcata ttcggcggca gcaaaagcgc tggttgacaa cattaagccc
    21181 acgagtgtgc cgctgaaata tttcagatat ttattcataa tcatgttctc acggtaaggg
    21241 gagaggtaac tccgcgatgg cggaattacc tttttcttat ttcagcgcgc ctggtgagaa
    21301 gaaatcaaca attgcgccgg ttttttgcat attaatatta gcttgctcaa tgttgagctg
    21361 ggcgacagag acaaaaaacg cgtccagcaa agtcagttgt aatattcttg ccgaggcatt
    21421 acgacctaat aacggtgttt ccggggccgg tgagcaaata atataatcgg ccagcttcgc
    21481 tattggtgaa tgatagctat gcgttataca aataatcttt gccccgttct tttttgccag
    21541 ttctacagcc gcttttacat cactggttcg cccggaatga gtcaccacca gcaccacatc
    21601 tccttcctgt aacaacgaag cagacatcat catgatgtga gcgtccggat aggcctggca
    21661 gcgtacgcca atgcgcaaaa acttgtgctg tacatcagca cagatagcat ttgatcctcc
    21721 ggcaccgtac aaatcccgct gtctggcctg atagaaaaag cgggcggcac ggtggatctc
    21781 atcaacgttg acgatcgact gaccttccat aatggtgcgt aaagtgatgt taaatacctt
    21841 attcaccaca tcctgcggcg cttcatcaaa agccaactcg gaaggcaata cttgttctga
    21901 ctgagaaaaa taatcttcca gcgcactgcg taagttacga aagccgctaa accccagcag
    21961 ctttgatacc ttaacgatca tcgcttcaga taccgccaga gcttctgcga catctttaat
    22021 tgcgggtgca caactcaggt taccgggttt gagtaaccac tccacgatgc ggctttcgtt
    22081 ttctgtcatt ccttcctgct tcattcgcag gtaaggcgct aacccgatac cgttcggaag
    22141 cgctgaatca aactctgact ggctcatcac gttctcttgt ccttgaattg aagacgccac
    22201 tatatcacca cagcaagaac agaaaagatg ctggtaactt cacaaaaaaa taaaatttat
    22261 aaagtttgtt ttgttgt
//
LOCUS       DADTFG010000079        22179 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.079, whole genome shotgun sequence.
ACCESSION   DADTFG010000079 DADTFG010000000
VERSION     DADTFG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.079"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(44..775)
                     /gene="rlmB"
                     /locus_tag="J1138_002762"
     CDS             complement(44..775)
                     /gene="rlmB"
                     /locus_tag="J1138_002762"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBA8454534.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(867..3308)
                     /gene="rnr"
                     /locus_tag="J1138_002763"
     CDS             complement(867..3308)
                     /gene="rnr"
                     /locus_tag="J1138_002763"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBA8454535.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKVGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSVKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3347..3772)
                     /gene="nsrR"
                     /locus_tag="J1138_002764"
     CDS             complement(3347..3772)
                     /gene="nsrR"
                     /locus_tag="J1138_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBA8454536.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3977..5275)
                     /gene="purA"
                     /locus_tag="J1138_002765"
     CDS             complement(3977..5275)
                     /gene="purA"
                     /locus_tag="J1138_002765"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBA8454537.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5379..5576)
                     /locus_tag="J1138_002766"
     CDS             complement(5379..5576)
                     /locus_tag="J1138_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBA8454538.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5658..6662)
                     /gene="hflC"
                     /locus_tag="J1138_002767"
     CDS             complement(5658..6662)
                     /gene="hflC"
                     /locus_tag="J1138_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBA8454539.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6665..7924)
                     /gene="hflK"
                     /locus_tag="J1138_002768"
     CDS             complement(6665..7924)
                     /gene="hflK"
                     /locus_tag="J1138_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBA8454540.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8010..9290)
                     /gene="hflX"
                     /locus_tag="J1138_002769"
     CDS             complement(8010..9290)
                     /gene="hflX"
                     /locus_tag="J1138_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBA8454541.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9367..9675)
                     /gene="hfq"
                     /locus_tag="J1138_002770"
     CDS             complement(9367..9675)
                     /gene="hfq"
                     /locus_tag="J1138_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBA8454542.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9761..10711)
                     /gene="miaA"
                     /locus_tag="J1138_002771"
     CDS             complement(9761..10711)
                     /gene="miaA"
                     /locus_tag="J1138_002771"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBA8454543.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAVGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10704..12551)
                     /gene="mutL"
                     /locus_tag="J1138_002772"
     CDS             complement(10704..12551)
                     /gene="mutL"
                     /locus_tag="J1138_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBA8454544.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGANQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGSRPAASWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKVPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12561..13898)
                     /gene="amiB"
                     /locus_tag="J1138_002773"
     CDS             complement(12561..13898)
                     /gene="amiB"
                     /locus_tag="J1138_002773"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBA8454545.1"
                     /translation="MMYRIRNWLVATLLLLCAHVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13917..14378)
                     /gene="tsaE"
                     /locus_tag="J1138_002774"
     CDS             complement(13917..14378)
                     /gene="tsaE"
                     /locus_tag="J1138_002774"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBA8454546.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14350..15897)
                     /gene="nnr"
                     /locus_tag="J1138_002775"
     CDS             complement(14350..15897)
                     /gene="nnr"
                     /locus_tag="J1138_002775"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBA8454547.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGTAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15896..17035
                     /gene="queG"
                     /locus_tag="J1138_002776"
     CDS             15896..17035
                     /gene="queG"
                     /locus_tag="J1138_002776"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBA8454548.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17018..17071)
                     /locus_tag="J1138_002777"
     CDS             complement(17018..17071)
                     /locus_tag="J1138_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454549.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17305..17380)
                     /locus_tag="J1138_002778"
     tRNA            complement(17305..17380)
                     /locus_tag="J1138_002778"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17345..17347),aa:Gly,seq:gcc)
     gene            complement(17532..18080)
                     /gene="pgsA"
                     /locus_tag="J1138_002779"
     CDS             complement(17532..18080)
                     /gene="pgsA"
                     /locus_tag="J1138_002779"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBA8454550.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(18137..19969)
                     /gene="uvrC"
                     /locus_tag="J1138_002780"
     CDS             complement(18137..19969)
                     /gene="uvrC"
                     /locus_tag="J1138_002780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBA8454551.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(19966..20622)
                     /gene="uvrY"
                     /locus_tag="J1138_002781"
     CDS             complement(19966..20622)
                     /gene="uvrY"
                     /locus_tag="J1138_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBA8454552.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            20918..21094
                     /locus_tag="J1138_002782"
     CDS             20918..21094
                     /locus_tag="J1138_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454553.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            21081..21305
                     /locus_tag="J1138_002783"
     CDS             21081..21305
                     /locus_tag="J1138_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBA8454554.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(21373..22095)
                     /gene="sdiA"
                     /locus_tag="J1138_002784"
     CDS             complement(21373..22095)
                     /gene="sdiA"
                     /locus_tag="J1138_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBA8454555.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         5230 a         6181 c         5436 g         5332 t
ORIGIN      
        1 aaaaaccatc caaatctgga tggcttttca taattcagag gaactagctg cgctgacgaa
       61 ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt cagggaagaa acacttcctg
      121 ccattgggat gctgatcaac tcatcgcaat gttcacgggt caggcgacgc ataccttcac
      181 cttccgcgcc catcaccagc gccaggcgtc cggtcatttt gctctgatag agtgtatgat
      241 ccgcctcgcc tgccgtaccg acgatccaga tattctcttc ctgcaacata cgcatggtgc
      301 gcgccagatt agtcacccga atcagcggaa cgctttctgc cgcaccacag gccacttttt
      361 tcgccgtggc gttgagctgt gcggagcgat ctttcggcac aatcaccgca tgaacaccag
      421 cggcgtccgc gctacgcaga cacgcgccga ggttgtgcgg atcggttacg ccgtcgagga
      481 tcagcaggaa cggttgatcg agcgaagcga tcagatccgg cagatcgttt tcctgatact
      541 gccgtcctgg cttcacacga gcgataatgc cctgatgcac ggcaccgtcg cttttctcat
      601 cgagatattg gcggtttgcc aactggataa ccacgccctg agactcaagg gcgtgaatca
      661 gcggtaacag acgtttatct tcacggcctt ttaaaataaa gacttcctga aaacgttcag
      721 gggcgcgctc cagcagggcc tgcactgcat ggatgccgta aatcatttcg ctcattaatg
      781 tactcgttgt ttacgttttg ccgggagcgc cacgcttgcc cggcctacgg ataattcacc
      841 ctcttctgtt tgaagagggt gaattatcac tctgccactt ttttcttcgc cgcacgcttc
      901 gctttggtcg ctgcggctat tttctgcgtt ttcaccgatg gctttttcgc ttttctcgcg
      961 tctttcttcg ctgctttcgg cttcggtttt ttctcgccgc gaaaggcgct gtctggctca
     1021 aagttgacct ttttgccgac ctgacgccgc ttgccgcctt ttttgcctgc atcgcctttt
     1081 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcgct ggagatcagg
     1141 ctaaagtcga ttttgcgctc gtccatatta accgcttcaa cgcgaacttc cacgcgatcg
     1201 cccaggcgat aagtctggcc gctggattca cccatcaggc gttgccctac ctgatcaaag
     1261 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1321 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1381 acctggtcga gcatgaagtc acacttcagc cagtcagaca catcgcgcgt tgcttcatcg
     1441 gcacgacgtt ccgccatcga acagtgctga cccagttgca gcatctcttc catcgaataa
     1501 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatat
     1561 ttaatggcgc ggtgcaacgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1621 taggactgca atgccagacc gaagtgacca cggttttccg gatcgtaaat cgcctgtttc
     1681 atcgagcgca aaagcatggt ttgcagcatt tctgcatcag gacgatcggc aaccgattcc
     1741 agtaactctg catagtcgcg cggttccggc ttgttaccgc ccggcagttc cagccccagc
     1801 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1861 aacagtgccg gttctttggc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     1921 atgcactctt caattaactt gtgggcgtcg ttacgctgag tctgttcgat acgttcaata
     1981 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2041 tcttcacggg ctttatccag cactttatag aggttatgta actcttcgag atgcttaacc
     2101 agcggcgcat actgctcgcg cagatcctga tcgccttgca gtatatgcca gactttggtg
     2161 taggtcagac gcgcgtgaga actcatcacc gcttcgtaga atttgtagcc agtcaggcgg
     2221 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2281 gagcacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2341 accgacgtac cacgattacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2401 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcacagtaa
     2461 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2521 agatcgacac ggcccacttt tgcttcttcc ggcacttctt ctttcagccc ggcaacctgt
     2581 tgctcaacag cctgcggcca gatgtacgga atttcatggg tacgcagagc gatatcaacc
     2641 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggta
     2701 cggcgagtcg ggcgttgagt cagttcaacc accaccacaa agcccatccg cgcgcccatg
     2761 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2821 ccgacgcccg cttcggtgaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     2881 cggacaatac gcgcttcacg acgaccttta cggtcagcgc ccagcggctg agccagcacc
     2941 tgatcgccat gaatgcaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3001 ccttcaactc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3061 tcgaggcgtt ccggcagcgc atagcactga cggcgagtga agaccagttg accatcgcgc
     3121 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3181 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3241 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3301 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3361 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3421 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3481 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3541 caccaatacg tattgcactc gccggtttac ccaagcgaat gccgccattt tttccacgaa
     3601 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3661 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3721 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3781 caaaagggaa atcggggaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3841 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     3901 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     3961 gccaggcgta ccagaattac gcgtcgaacg ggtcgcgcag aatcatggtt tcagtacgat
     4021 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaatg cgcttgatgt
     4081 agttcagtgc cgcctgcggc aggccgctac gatctttcac gccgaaggtg gattcagacc
     4141 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4201 tggtagtcac ttcacgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4261 ggccatccag aacgtccagt ttagtcaggc agaagccaga cagagagttc agctgtaccg
     4321 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4381 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4441 acggacctgc acccacacga gtggagtaag ctttgaggat acccagaacg taatcaacat
     4501 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4561 cgtacggata agtgccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4621 cgaaatcgcc acgctgacgc gcctggtcga gcagatcaga aacgtcaaca accatagaag
     4681 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4741 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4801 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgcc actttatctt
     4861 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     4921 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcggacagca
     4981 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5041 cagccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5101 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5161 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5221 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5281 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5341 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5401 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5461 tcgggcaaat tggtcattgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5521 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5581 tatccttata gaaaaagaaa accaccgaca tccctgagga tgtggtggct ttattgacct
     5641 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5701 tcgctgtccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5761 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5821 ttggctgctt ctgcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     5881 agcgttctgg tcacttcata gtcggcagtt gcacgcagtt tttccgcttc ttcctgacct
     5941 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6001 tcagacactt cggtcggcag gttgatctgc ttaattcgca catcgacaac ttcaataccc
     6061 agcgccgcca tactgttcgg attgatgacc ggaactttgc ccttcgtctc agccgttacg
     6121 cgctctgccg cttcggcaat ggcgttatct gctgcagggg tagtaacttc atcttctgta
     6181 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgagaatcg
     6241 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6301 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag atagtaacgg
     6361 ctgaaatcgc tgatgcgcca tttgatgtaa gagtcgacga tcaggtcttt cttctctttg
     6421 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6481 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6541 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6601 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6661 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6721 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6781 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6841 cacctttcag catctggtct aatggcagaa ccatcaggtt gccaccttta tcgttaacca
     6901 gcactttgcg ggtgttgccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     6961 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7021 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7081 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7141 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7201 gagcagcctg gaagttgacg tccagcagag tgatgcccat gtcatacgga cgaatcgtct
     7261 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7321 ccatggtgta tttaccgata accccgcgca gggcgctgtc ggtagcctga cgcagactgt
     7381 catccgggct ggtcacgcta tacagatatt tttccggatc ggtgacgcgg tactgcacgt
     7441 tcatctcaac gcgcactacg ttctcgtcag acgtcagcat cacaccagaa gctgccagtt
     7501 cacgcacggc ttccacgttc accggtttga cttcgtcaat aaacgtcggt ttccagttca
     7561 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7621 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7681 taacgacgcg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7741 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7801 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7861 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     7921 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     7981 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8041 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8101 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcaggcgc
     8161 ccttcctgcg gcggcagacg caatgtatgc tgcgccacct cgccggaaag acgctccgtc
     8221 aaagcctgaa aaagctgtgg tatccccgct ccggtctgcg cagaaagcca gacacggatc
     8281 ggtttgttct cttcatctct gtcaatacgc ggttcgaaat cgtccagcat atcgatcttg
     8341 ttcatcacca gcagggttgg gatctcgtga gcatcaatct cttcaagaac cgtattcacc
     8401 gcttcgatgt tttcttgtac acgcacatcc gccgcgtcaa tgacgtgcag cagcaatgtg
     8461 gcttgccgcg tctcttgtaa cgtggcttta aatgccgcca ccagatcgtg cggcaggtgg
     8521 cgaataaacc ctacggtatc tgccaacacg gtttcaccga catccgcaac gtcaatacgc
     8581 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8641 atgcgattga aaagagtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8701 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttccagg
     8761 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8821 tcacccggac cacgcaaacc aatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     8881 accaggcgcg tagccagatg gcgcaactgc gccaactcaa cctgcaactt accctcatgg
     8941 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat gacacgacac
     9001 tcgcacaaac gctccaggtt acgctcctgc gccgggctca gggcatggtc aaaaagaacg
     9061 accgaagctc ccgtcgcttt tacggcttcc gcaatttcaa ctgctttacc ttcacctaca
     9121 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9181 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9241 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9301 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9361 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9421 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9481 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9541 caggatcacg aactgatcaa aagactcgat ttgtccttgc agcttaatac cattcaccaa
     9601 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9661 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga atctttcgat
     9721 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9781 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9841 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
     9901 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
     9961 aacccacgca acgaatggaa ggcaaatccg tatgcaaatc tcctcgggca aaaagcgccc
    10021 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca attcgttgat
    10081 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10141 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10201 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatcgatct
    10261 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10321 cccgtacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10381 acaacatcgt accgcccact aacagtggga tccgccccac cgcggtgata tcggccattt
    10441 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10501 tcagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10561 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10621 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10681 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10741 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10801 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10861 tttcgtgcaa tccactgcgc aatatttcca ggttcgaata cggactgctt cgccaggtag
    10921 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    10981 ctgatggtca catgctgcgc atcggactgg aaatcaatac ccagttccgc cagggcagac
    11041 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11101 agcggttggg cgcaaactgg cacttcaccc ggcgtcaatt gtgcctgacg cagccaacgt
    11161 tctgccactg gcaaggataa aagtgaaatg ttgccatcgc gctcaagcaa cgcacagtcg
    11221 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11281 tgcggttctg gcacttttgg tttttgcatc ggagcgggcg tttgcaaaag ctggcgatac
    11341 acttcacctt gctgtttctg gtaaccaggc tgcgcattcg gccaggaggc agccggacga
    11401 ctgcctgagg cgggcgcagg agagtagcgc ggagctaccg gctcacgaac tgccggttca
    11461 gcaaagtgat tgcgcccagc cgccacgcga ttttccggaa tggcacgcgg tgcaggttgt
    11521 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11581 tgatagataa agtcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcaggg
    11641 tgcacgttga cgtccacctg atgcgggtcg atctccagat acaacacaaa tgccggttgc
    11701 tgattggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11761 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtagtgtga
    11821 tttggatcgg ccacccagcc gcgcagcgtg agatcgccgt gttgccattc aatcgccagc
    11881 gcctgttcga gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    11941 tccggcactg cgcggtactg gcgtacaatt ttgccgttat gcgacaggtt gatagtgacg
    12001 tcgaaacgcg ccagcgcaat gcggcggatg atttcatcga tatggttaaa ttcggttttc
    12061 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acaggtccag cacctccagc
    12121 gtcgttccca ccggatgcgc cgccggttta accgtcacgt ccatatcgcg cccttcggca
    12181 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12241 gaactgatac tcgccagcgc ctcaccgcga aagcccagac tgataatggc ttcgagatcg
    12301 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12361 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccgccacg ttcgatatca
    12421 atatcaatgc gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12481 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12541 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12601 gccgtttgtg ccgttgcacc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12661 cgcaggcctt tataaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12721 cgttcttcgc tgttgttact gataaaaccg gtttcgacca gtactgacgg aatatccggc
    12781 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccgatgcgt
    12841 tgcaactgac tgatcatact ggtcgccaca tcatacccta cccgctggga atgaccgaac
    12901 tgtaaatcca gcaccgcctg gcttaaatac gggtcagact gactgttcgc cagcacatcg
    12961 cccgctccgc ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13021 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13081 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13141 cgccccatca ccgagataaa gtaatcaccg tcacgggtta aaacgccctt aaacatcgga
    13201 tcgtcattga gcaaagtacg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13261 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13321 ataattttat cgccagtgtt agccgtcgcg cgcgcagccg gtcgcgttac cgtattactg
    13381 ctgataacac ccgtagtgcg gttactttca gttttaaacg gattgcgcgc tggttcactg
    13441 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacagg cggcggagga
    13501 ggcggtggcg gcgcatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13561 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13621 gcatctttag gcgttccgga gcgaatcgcc ttcaccagat tattaccgct gaacaacaat
    13681 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13741 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13801 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca cgtgcgcgca cagcagcagc
    13861 aacgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    13921 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    13981 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14041 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14101 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14161 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14221 cccagagcct gtaaaaagcc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14281 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14341 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14401 gttaacaata cgctgtagcg tggaaaagag atcggtcgcc agcattccgc gcgttccaaa
    14461 acgcgccgcc agtacgtcag ctgccgtacc gtgtgcgaca cagcccgcgc acgctgcatc
    14521 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14581 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14641 ggcgaccacg gttccggcac ctttcagcac cgctacaccg ccataacgtt gtaccagacg
    14701 ttgggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14761 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14821 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    14881 ggcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    14941 ttccaggctt tcggtaagag agtccatagt cagttcatgc accatcaatt ccggtcgtgc
    15001 agtcagcagc ggcgcaatgt tctcactgcg ggtcagtact cggactaaac cagcaccagc
    15061 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccaat
    15121 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggta tcagccactg
    15181 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15241 cagccccagt gagtcaaaat gcagttgtcc ggtaacatcc cgcgcttttc cagtgagcaa
    15301 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgattaccg cgcctggcgt
    15361 agcgccggtt tcagccagaa ggccggaagg gatatcaacc gccacaatcg gcgcagggtg
    15421 agtattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15481 gccgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15541 gatctcgcct cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15601 tttgtcactt tcctgggcca gcagcgtgac atcaataccg atcgctttgg ccagccgcgc
    15661 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15721 gtcaggataa gccgaacgac acacctggaa tgcggcttcg ccagcgcgaa gcatcagctc
    15781 atagagtgtg agccccagcg catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15841 gtaccagact gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    15901 agagcccctc gatctcaatc aattagcgca aaatattaaa cagtgggggc tggaactggg
    15961 ctttcagcag gtaggtatta ccgataccga tctcagcgcg tccgagccca aactgcaagc
    16021 atggctggac aaacaatacc acggcgaaat ggactggatg gcgcgtcacg gtatgctacg
    16081 tgcccgcccc catgagttat tgcccggtac gctgcgcgtg atcagcgtgc ggatgaatta
    16141 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16201 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aatcgactca aaaagctggg
    16261 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16321 tattctcgag cgcccgttag ctgaaaaagc aggacttggc tggacaggta agcactcact
    16381 tatcctcaat cgcgaggccg gttcgttctt ctttttaggc gaattgctgg tcgatattcc
    16441 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcgtggcct gtatgacgat
    16501 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16561 tctcaccatc gaactggaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16621 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16681 tacagaagac gatttcagcc cgcgtaagcc gctacacgca ccggaactca ttgagttatt
    16741 cgcctggagt gaagagaagt ttttaaaagt cacggaaggt tcggcgattc gccgtatagg
    16801 tcacctgcgt tggctgcgta atatcgccgt agccttaggc aatgccccct gggatgaaac
    16861 gattttgaca gcgctggaaa gtcgtaaagg tgagcaccca cttcttgatg agcacatagc
    16921 gtgggcgatt gcgcagcaaa tcgagaggcg aaatgcgtgc gtggtcgaag tgcaattacc
    16981 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17041 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agccggaaaa aatcgtcaag
    17101 tgatcgatac gacaaaacga aatcttaatt tttaacttat ctatcaatag gttaaaaaaa
    17161 tactattcac cgtgcgaagt ttctttgaaa ttaagattac ggcctgaggc tgtggataac
    17221 tctgtttaca agagtctgtc agagacatca aaaaccagca ataacgcatc gcgtcgctgt
    17281 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17341 ccttggcaag gtcgtgctct accaactgag ctattcccgc attcatcaag caatcagtta
    17401 atcacttgat tttattatcg tctggcaatc agtgccgccg ttcgatgcgt tgcattctac
    17461 ttacctggcg cgatgagtca acgatatttt tcaccacttt tgatcgtttg ctgaaaattg
    17521 cgccgaaacg atcactgatc aagcaaatct gcacgcgcag cgctcaaata ttgcaacatt
    17581 gaccacagag tcagtaccgc agccacaaag aaaagtgcaa taccggcgta ctcaacccaa
    17641 atgttcggac gccacagcag ccaggctaac gccaccatct gggcagtggt tttcactttc
    17701 ccaatccagg agacagccac gctactgcgt ttacccaact ccgccatcca ttcgcgtagc
    17761 gcagaaataa taatttcacg ggcgatcatc gttgctgccg gtaaggtcac ccaccagctg
    17821 tggtaatgct cggttaccag caccatggcg atagccacga gaactttatc tgcgacaggg
    17881 tcaaggaaag caccaaaccg ggtactctga ttccagcggc gtgccagaaa accatcgaac
    17941 cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg caaacggcga ccaggtgaca
    18001 ggcaaataaa agaccaatac aaagaatggg ataaggatga cacggaacag tgtaagcaac
    18061 gtagggatat taaattgcat aatgacgggt aactatctgt tgtcagtaag attaccccta
    18121 tgttgctaca gagacatcaa tgtttcaacg accagaagat cttttctgcc agaccttgcg
    18181 aaatacccgg cacttttgca atttcctcga cgctggcgtt acgtaaacct tgcaaaccgc
    18241 ccatatattt caacaacatt tgccgacgtt ttggcccgac gccttcaatg gtttccaggg
    18301 aactggtatt tttgaccttc gcccgttttt tacggtgccc gccaatcgcg tgatcgtgtg
    18361 attcatcgcg aatatgctgg ataacatgca gcgcgggaga atctggcggc aaactaaatc
    18421 cctcaccttc aggctcaaag aacagcgttt ccagcccagc cttacgatct gctcctttgg
    18481 caacgccaag tagcagcgga tgatttttat cccatgagac atccagttcg gcgaaaacat
    18541 ttttcgcctg cgcaagctgg cctttgccgc cgtcaataag gatcacatcc gggatcttac
    18601 tgtcgtcgat agctttacca taacgccgac gcagcacctg attcatcgcc gcgtaatcat
    18661 cgcccggcgt gatgccagta atgttatagc gccgatactc cgcacgcagc gggccgttgg
    18721 catcaaacac cacacaggaa gcgacggttt gttcacccat ggtatggctg atgtcaaagc
    18781 actccatccg cttcacttcc ggcaatttca acacgctggc aagcgcggtc agccgctggt
    18841 gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt cgccgcattg gtacgcgcga
    18901 gtttcagata acgcgctcta tcgccacgag gtttggtttg aacattaatc ttgcgtcccg
    18961 ccagttctga aagggaatcg gcgagcagcg ttttatcgct aagattaaaa tcgagcagga
    19021 tctcacccgg taaggtgcgc atctggctgc cttgtaaata gaactgacct acgaaggttt
    19081 ccaccacctc gctcagttcc gtaccgccag gcactttcgg gaaatagctg cggctgccga
    19141 gcactttgcc ctgacgaatg aacaatacgt ggacacaagc catacccgca tcgaacgcca
    19201 ctccaataac gtcaaggtcg tcgccggtat tggaaacaaa ctgtttttcg gtgacacgtc
    19261 gcaccgcctg aatttggtcg cgaatccgcg ccgcttcttc aaactccaga ttctggctgg
    19321 cagtttccat acgactaatg agttgcgtaa gcacctgatc atctttgcca gacaaaaaca
    19381 ggcgcacata ctcgacctgc tgagcgtatt cttcttcact caccagtcct tcaacgcacg
    19441 gccccagaca gcgccctatc tgatattgca gacacggacg cgagcggttg cgataaacac
    19501 tgttttcgca ctggcgaatg gggaaaatct tttgcagtag cgccagtgtt tcacgtacgg
    19561 catagccatt cgggaacggg ccgaaatatt cacctttggc atgcttcgct ccacgatgca
    19621 tcgccagacg cgggtgggta tcgccactca ggaagataaa aggatatgat ttatcatcgc
    19681 gtagcaaaac gttgtaacgc ggctgataaa gtttgatgta gttgtgttcc agtaacagcg
    19741 cttcggtttc tgtatgagta accgttacat caatttgctg gatctgggcg accagcgctt
    19801 cggttttgcg cgaagcgagg ttgctacgga aatagctgga aagccgtttt ttcaggtctt
    19861 tcgctttgcc gacatagata accgtaccac cagcatcgta catgcgataa acgcctggct
    19921 ggctggttac ggtttttaaa aacgcttttg cgtcaaactg atcactcact gacttgataa
    19981 tgtctccgca ttacacagac catggcgaat tgccaggtga gtcagctcaa catcgccatg
    20041 aatgtttagt ttactgaaca tacgatagcg gtagctgttc accgttttcg gactgagatt
    20101 gagctgttct gagatctcat tgaccttctg gcccttggtg atcatcagca taatctgcaa
    20161 ttcacgttca gacaaactgg caaatgggct ttctgttttt tctggttcga tctggcttaa
    20221 cgccatttgt tgagcgatat cagaagcaat gtaacgctgc cctgaataga cagaacgaat
    20281 cgcactcacg acttcctgcg gagccgcgcc tttgctgagg tagcccgcag ctccggcctg
    20341 catgactttc gctggtaaag ggttttctgt atggacggta agcatgatga ttttgacatc
    20401 agctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg ccaatgcccg gcatactcat
    20461 gtccattagc accacgtcaa cggcatttgc ccggcaccat ttaacggcgt cttcaccgca
    20521 cgatgcctca ccgacgactt ttataccctt tatatcttcc agaatgcgtc gtatccctgc
    20581 gcgcaccagt tcgtggtcat caacaagtag aacgttgatc aaaggaatat ctccagaaat
    20641 agggataacg ctactgatag ttagtcattc gtatattagc ggtttttatt gcaactttga
    20701 aacgttaaaa atggtgggta ttcgattttt ctctcgtttt tgtcaattcg attgtccaca
    20761 gttatggtca cgcccgtcac caacggcttc cgggatcgtt tcacagtaag cccggcgatg
    20821 aaatgtgaca aaaattgact ttattcagca aaaatgaaaa tcagcccgct gaatatgtaa
    20881 cattaattaa cccgaacaat acgggcaaaa aacattcatg ataaaaatat ttattggtca
    20941 ttatattaac gtattttata gtactgttga tatcacgctc aaaaaacaac cactgctatt
    21001 tttagcaaag cttatggtat actccgccgc cttaacattt ttcaccgcaa attttcattg
    21061 caacatgacg aggaaaataa atgagtacgc ctgatttttc tactgccgag aataatcaag
    21121 aactggcaaa tgaagtctcc tgcctgaaag cgatgctgac gctgatgctg caggcgatgg
    21181 gacaagctga cgcgggccgc gtgatgttaa agatggaaaa acagcttgcg ctgatcgaag
    21241 acgaaaccca ggctgcagta ttttccaaaa cggttaagca aattaaacaa gcctaccgtc
    21301 agtaatataa aaccggctga gagcgtgcct ttcagccggt ttttgcatct ggcacgcaga
    21361 acagaaaaga gatcaaatta agccagtagc ggccgcgtaa caggcaacct gcgtcttatt
    21421 cggtgcattg atttttttct gcatgttttt ctgatggaaa ttgaccgtat tctcagagat
    21481 tgacaaaatc atcgctatct ctgctgatgt cttaccttcc gccgtccatt tcagaatttc
    21541 tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact atttcatcat ttaaacgcat
    21601 cagagccatc agactttcgc gcaccagtag ctgcattttt aattgcagtt catcactaag
    21661 aatgggtatt tcgcgcgtgc tgcaacggga aaaggacaaa aagcccaacg cccgatttgg
    21721 caacattaaa tactgagtga caccgcggcg taaaccatgt gcgcgcgcag cttcccataa
    21781 cggctgtgct tcgctgaata agtcatcatt ccacattaaa tggccctgac taaagttttc
    21841 agggttcagc accggatcaa ttgcgagaaa attttttgcc tgataataac taacccacga
    21901 ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg aatggtaccg ggtggcgaac
    21961 acataacgaa tagtaatcgt actccagctg ctgagcctga agctctattt catggtagac
    22021 ctcttctgcg gcctccatcc tctgaaaacg caacaacatc gtgcgaagcc agctgaaaaa
    22081 atccgtatcc tgcatagtaa accgcaacgc ccctgagagt gagtatcatt tataatgata
    22141 attcaaacta tcacataaat aagatttata tataattta
//
LOCUS       DADTFG010000080        22108 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.080, whole genome shotgun sequence.
ACCESSION   DADTFG010000080 DADTFG010000000
VERSION     DADTFG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.080"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(99..806)
                     /locus_tag="J1138_002785"
     CDS             complement(99..806)
                     /locus_tag="J1138_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBA8454556.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1205..3340
                     /locus_tag="J1138_002786"
     CDS             1205..3340
                     /locus_tag="J1138_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBA8454557.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGANWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3389..4644)
                     /locus_tag="J1138_002787"
                     /pseudo
     CDS             complement(3389..4644)
                     /locus_tag="J1138_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
     gene            complement(4846..5925)
                     /gene="mltC"
                     /locus_tag="J1138_002788"
     CDS             complement(4846..5925)
                     /gene="mltC"
                     /locus_tag="J1138_002788"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBA8454558.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5990..6265)
                     /locus_tag="J1138_002789"
     CDS             complement(5990..6265)
                     /locus_tag="J1138_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBA8454559.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6293..7345)
                     /gene="mutY"
                     /locus_tag="J1138_002790"
     CDS             complement(6293..7345)
                     /gene="mutY"
                     /locus_tag="J1138_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBA8454560.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMVRFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAVLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQISADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7506..8225
                     /gene="trmB"
                     /locus_tag="J1138_002791"
     CDS             7506..8225
                     /gene="trmB"
                     /locus_tag="J1138_002791"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBA8454561.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8225..8551
                     /locus_tag="J1138_002792"
     CDS             8225..8551
                     /locus_tag="J1138_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HBA8454562.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            8735..9454
                     /locus_tag="J1138_002793"
     CDS             8735..9454
                     /locus_tag="J1138_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBA8454563.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9630..10676
                     /gene="ansB"
                     /locus_tag="J1138_002794"
     CDS             9630..10676
                     /gene="ansB"
                     /locus_tag="J1138_002794"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBA8454564.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10793..11800
                     /locus_tag="J1138_002795"
     CDS             10793..11800
                     /locus_tag="J1138_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBA8454565.1"
                     /translation="MKKQWIVGTALLMLMTGNVRADGEPPTENILKDLFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVTILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(11865..13001)
                     /gene="hemW"
                     /locus_tag="J1138_002796"
     CDS             complement(11865..13001)
                     /gene="hemW"
                     /locus_tag="J1138_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBA8454566.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLTADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYSKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRPQLEEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(12994..13587)
                     /locus_tag="J1138_002797"
     CDS             complement(12994..13587)
                     /locus_tag="J1138_002797"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBA8454567.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(13595..13885)
                     /locus_tag="J1138_002798"
     CDS             complement(13595..13885)
                     /locus_tag="J1138_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBA8454568.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(13882..14448)
                     /gene="yggT"
                     /locus_tag="J1138_002799"
     CDS             complement(13882..14448)
                     /gene="yggT"
                     /locus_tag="J1138_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBA8454569.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(14466..15170)
                     /gene="yggS"
                     /locus_tag="J1138_002800"
     CDS             complement(14466..15170)
                     /gene="yggS"
                     /locus_tag="J1138_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBA8454570.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            15188..16168
                     /locus_tag="J1138_002801"
     CDS             15188..16168
                     /locus_tag="J1138_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBA8454571.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFTSGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            complement(16342..16758)
                     /gene="ruvX"
                     /locus_tag="J1138_002802"
     CDS             complement(16342..16758)
                     /gene="ruvX"
                     /locus_tag="J1138_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBA8454572.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQEY"
     gene            complement(16758..17393)
                     /locus_tag="J1138_002803"
     CDS             complement(16758..17393)
                     /locus_tag="J1138_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBA8454573.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(17430..18380)
                     /gene="gshB"
                     /locus_tag="J1138_002804"
     CDS             complement(17430..18380)
                     /gene="gshB"
                     /locus_tag="J1138_002804"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBA8454574.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYIEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(18393..19124)
                     /gene="rsmE"
                     /locus_tag="J1138_002805"
     CDS             complement(18393..19124)
                     /gene="rsmE"
                     /locus_tag="J1138_002805"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBA8454575.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(19204..19911)
                     /gene="endA"
                     /locus_tag="J1138_002806"
     CDS             complement(19204..19911)
                     /gene="endA"
                     /locus_tag="J1138_002806"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBA8454576.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(20006..20503)
                     /locus_tag="J1138_002807"
     CDS             complement(20006..20503)
                     /locus_tag="J1138_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBA8454577.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(20580..21974)
                     /locus_tag="J1138_002808"
     CDS             complement(20580..21974)
                     /locus_tag="J1138_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8454578.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQIMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5461 a         5582 c         5805 g         5260 t
ORIGIN      
        1 cgcattaaac ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta gtgcagactg
       61 cacaatctat catcactttg ccgctattgc acacattgtc aggcaaacca ggcggtccag
      121 gcggcggaaa gcggcattgc cagaaggagt gctggaagca tgttgaccac cgggaacatt
      181 ttaataccac agatgcgtaa tccggttgcc agcagcaata aaccgcctac ggcgctgaag
      241 tctgccatca tcgacggtgt ggtcagcggt aatatcagcg cggcagccca cgccagtgtt
      301 aactggatga tcagtaatgg aatgctaatc accgacaccg caatacccag cgagcaggcg
      361 aagatcatcg ccgtaaagaa atcaagaaat gacttggcga ttaaaatact cggatcgccg
      421 gtcatccctt cgttcatcgc cccgaagatt ccggtgccgc tggcgcaaaa caggacaata
      481 atcgcgacaa aattctgaat aaaagattca tgcgctggct tcttacgcga gtggtgaaac
      541 agattatgcg ctttggcgac cactgtattg acgccttttt ccagcagaca aatttcgccg
      601 attaatgctc cgagtaaggt tgctaaaacc atcgccggaa ggttggcgca tttcaccacc
      661 agtaaaatac caatccccag cgatgccaga ccaaaaattg atgtcatgga gacgcggata
      721 cgttccggta agcgttggct aaggagtgcg ccgaaaacgc cacccagtaa gactgcactt
      781 gcgttgataa aagggccgat gaccacaaga gttcctgtta gctgtattgt ttgattttct
      841 tattatgaac ttttggcgcc gtcgatggct tgtttgttcg caaagtcctg gcttgcacgc
      901 tttagcgaaa ggtgccatga ttgcgcgaat tttctcctct ctgtacggag tttgcccgat
      961 gcacgccacc tccttacatt ctctcgctta tcgccgtttc gcgcgaaact cttctctttt
     1021 tatgctactg ctcatgcggt gaagttaacg cacgctcact gcaggacaac agtaaaatca
     1081 gagcgtttct gcttttactg atgtctggcg gtcggagctg gtgaccagtt tgacccatat
     1141 ctcatggggc agggttttcc tccttgtccg gtattcttac ttcccctaaa cgggttttgc
     1201 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     1261 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     1321 cgctgcggat agtcgcagtg gcattcttgc attgcttaag cgcaccggtt ttcatctacc
     1381 ggtgtttttg tattccgaac atgctgttga attacctgca ggcgttacgg cggtaatcaa
     1441 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     1501 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggtaata gcacctttgc
     1561 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     1621 cgatttcttt ggcgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     1681 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaatttgcag ccaaagtctt
     1741 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     1801 gaatgcgctg ttaacgcgtg gcgatctggt gctcttcgac cgtaacaacc ataagtcgaa
     1861 tcatcatggc gcgttgattc aggcgggggc gacaccggtc tatctggaag cttcacgtaa
     1921 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     1981 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     2041 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     2101 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttacg aacagtttat
     2161 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     2221 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     2281 taaaaaagat aaccatatcc gtggacaggc gcgtttttgc ccgcataagc ggttgaataa
     2341 tgcctttatg ctccatgctt ctaccagccc gttctatccg ctgttcgccg ccctggacgt
     2401 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     2461 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     2521 gcccgttgtt gatggcaaat tgtggcagga ttatccgacg tcagtgttag ccagcgaccg
     2581 ccgttttttc agttttgagc cgggggcgaa ttggcacggc tttgaaggat atgccgcgga
     2641 tcagtatttt gttgatccgt gcaagctgtt actcaccacg ccgggtatcg atgccgaaac
     2701 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactacc tgcgtgagaa
     2761 cggcattgtg ccggagaagt gcgatctcaa ctccattctg ttcttattaa ctccggcgga
     2821 aagccacgag aagctggcac aactggtggc gatgctggcg cagtttgaac agcatattga
     2881 ggatgattcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     2941 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatttgtatg tcagtttcga
     3001 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     3061 tccccaggat gcgcatagcg cttatattcg cggtgaagtg gagttggtgc ggattcgtga
     3121 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     3181 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcgc tggaagaagg
     3241 cgtgaatttg ttgccgggat tttcgccgga gttgcaaggt gtctatagcg aaaccgatgc
     3301 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa acgggtcacc
     3361 ttctggcgac ccgtttttct ttgcgtaatt agtggctaac cgtctgtgta cctgtcggga
     3421 cacgaacgtg tttatatttg aacatcgcca tgaacgcgaa ggccagcacc acggagtaac
     3481 ctgcgaaaat cagccatacg gtctgccagt cggtaatgcc gttttgggtg tacatctcaa
     3541 ccactttacc gctcacgatg ccgccgagga tacagccgaa gccgttggtc atcatcagga
     3601 acatcccctg cgcgctggcg cgaattgccg ggctaacttc tttttcgaca aacaccgaac
     3661 cagagatgtt gaagaagtcg aatgcgcaac cgtaaacgat catcgacagt accagcagta
     3721 cagtaccgaa cggagtcggg tcgccgtaag caaacagagc aaaacgcagg atccacgcca
     3781 caatactgat catcattacg ttcttaatac cgtagcggct taagaagaac gggatggtca
     3841 gaatgaacag ggtttcagag atctgcgaaa tcgacatgat gattgacgca tgctgcacga
     3901 tgaagctgct ggcaaacatc ggatctttgt caaagctgtg caggaaggta ttaccgaaca
     3961 tgttggtaat ctgcagttcc gcgcccagca gcattgagaa gatgaagaag attgccatac
     4021 gcttgttttt aaacagcgcg aatgcatcga ggcccagcag ggttgtccag ctctgattcg
     4081 cttgctgttt agcaaccgga atatgcggca gagtcagggt aaacagaacc agaatggcgg
     4141 aagcgctgcg ccaatataca gctgcatgtg gcttaattcg aagccggaca ggctcaccac
     4201 ccacattgcc atgataaagc cgatggtgcc ccagatacgg attggcggga agtcagtaac
     4261 gatatccatc ccggcatttt gcaggcgata gtaagagatg gtgttgatta acccaagcgt
     4321 tggcatataa gcaaacgagt taatcaatat cacaaggaac atcgcctccg gcgtagtgac
     4381 ctctgccgcc atgaacagcg tgatagcgcc aatggtgtgg caaatggcat agacccattt
     4441 cgcacttaac catttgtcgg ccacaatccc cagcagcgca ggcataaaga ccgcggcgat
     4501 acccagtgaa ctataaactg cgccaataga agcaccgtca aacttcaggg taacaaacat
     4561 ataggagccg agggtcgtca gccaacttcc ccacagacag aactgcagaa aagagaggat
     4621 tttcagctgc agcttaagat tcatgttaat ttcctcacat cgtgatgcgg atgagtgttt
     4681 gaaaaagcac atttgaccgt gcgagatgat gggattctat caacttcgcg gactggtttt
     4741 ttgttacctg tggcaaataa ttgcaaacat tcccaatttg caaaacgaaa ttgtgatgtg
     4801 gataacattt ttacccctga gcatcgtcag gggcggttag tggaattatc ggcggcggta
     4861 ggatttttgc gcggtattca ctttataaag ataacggcga gattccgcag agggatggcg
     4921 ggtcgtcagc gtctgataaa catcgcccgg cgtcatggtg ttaataatat tggccgcctg
     4981 aatcttgtca ttcgaaaaga ctcgcagcac gctgcctgcg ccgccgttat aggcggtgat
     5041 gacggcataa cgccgcgatg ttgggttatc aattccgccg agataaacat tgttcagcat
     5101 cgccagatac gcggtgccgg tatcaatatt gctggcagga tcaaacaaga aactgcggct
     5161 cggcgtgccg gatttcccct gcgagcggaa cacatctttc ccggcagtat gttgtaccac
     5221 ctgcattaat cccagcgcat cggaacggct gaccgcatac gggttaaagg aagattcagt
     5281 ctgcataatt gccagaatca gcgactcatc aacgccatat ttgcgtgacg cctggcggac
     5341 catgccgaga tatttgtgcg cacgtttatc aaggtggttc ggcaccatgt taatggtgac
     5401 gctgtagatg atacgcagcc cgttgctgcg gctcttcaga cggtttttca gcagataatc
     5461 cgcgaagttg cttgcgcgac cttcccagcg aatcggctgc ccggtgttgt ccaccacctg
     5521 accgtaaagg aaaggttctt tcgaaatcgt aatatcatca acgtcggaat agagatcgac
     5581 cgaactcgga tcgtcaccca tcagcaacgt tttgataatt gcccggcgca aatgcgcggc
     5641 aggttctgtc ccggcgatag tttcgatagt aatcgtaccg tcatcgaagt tgatgtggct
     5701 gcgggtttga tattgatcgg tgtatttcac gtagtcctta ggaccggcga tcaccacctc
     5761 tttgaagccc cagatgttct caatattgtg ggcaaattgc cccatcagaa tatcaaaacc
     5821 gttggtatct ttgacccagg cttcgttata ggtatcgcct tttttggtcg tcgaacagga
     5881 gatgagcaac ggcgcaatca aagccagcgc gagatatttt ttcatcattc cgggtgcgtg
     5941 ttgtgtttgt ttttatgcaa gttgccggag gcgtgctccg gcactgtttt tattttttat
     6001 cttccggcgt atagccctcg atatgcacct ctttaccctc gaacaggaag ttgaccatct
     6061 cctgctccag cagcttgcgg tgctcggcat tcatcatgtt gagtttcttt tcattaatca
     6121 gcatggtttg tttgtgctgc cactgcgccc aggcttcttt ggagatctcg ttatagatgc
     6181 gttttcccag ttcgccgggg tacagctgaa aatcctgacc ttctgcttca cgttgcagga
     6241 aagtacaaaa aatcgttctg ctcataaatc atcctcttta tcgactcacg cgctaaaccg
     6301 gcgcgccagt gcgtaactgc tgtaacaaac gctccacggg agccgccagg ccaactgacg
     6361 gcggttgcgc taagttatac cagagcgcat tgtcttcatc catgcagccg gtgaatgacg
     6421 acacgggaag ccacataggc acaatatcta agtggaaatg gctgaaggta tgccgaaacg
     6481 cggtcagctg cgtcaggtta tcggcagaaa tctgtcgttg cgccagccac tgccgcaaac
     6541 tttcttcatc ggcaaactgc gggaaacagt ataaaccgcc ccacaatccg ctcggcggac
     6601 gctgcgccag caatacttca tcttcgtgct gtaacagcaa aaagtagccg gtgcgctctg
     6661 gcagcgtctg tttcggtttt ttgcccggat aaagcgacca gctattgttg gcggcggcaa
     6721 tacatccgtt ttgtagcgga cagagcgagc atttcggctt agagcgcgta caaatcatcg
     6781 cgcccaaatc catcatcgcc tggttaaacc gttccacgcc aaccgcgggc gtcacctgct
     6841 cgctcaaact ccatagttta ttctcgacct cttttttccc gggccagccg cttacagcat
     6901 agcagcgcgc cagcacgcgt ttgacgttac cgtcgagaat cggaaagtgc ttacccagag
     6961 aaagcgagag aatcgcgcct gcggtggaac gcccgacgcc cggcagtact gcaacttcct
     7021 caaaggtttc cgggaattta ccgccgtgta aggccgccac ttgttgtgcc gctttatgca
     7081 gattgcgcgc tcgggcgtaa tagccaagcc cggtccacaa gtggagaact tcgtcgagcg
     7141 gcgcattggc gagatcggtc accgttggga agcgcaccat aaagcgttca aaatagggga
     7201 taacggtcgc aacctgagtt tgttgcaaca tcacttctga gagccatact ttgtagggcg
     7261 tcttgtcaat ttgccagggc agcgttttcc gcccgtattt atcgtaccag tccagaacct
     7321 gggctgaaaa ttgcgacgct tgcatggtca ccgaattcac tgttgttggg ggcaagattg
     7381 cagcacagcg acggcagggt gtaaaccgga actttccgca gccacggcct tatcatgctt
     7441 gcatccggca actaactttg gataatgccc gttttcagaa cactttcaca agcgactaaa
     7501 tctttatgaa aaacgacgtc atctcaccgg aatttgatga aaacggccgc ccgctgcgcc
     7561 gtatccgtag ttttgtgcgc cgccaggggc gactgaccaa aggccaggaa catgcgctgg
     7621 aaaactactg gccggtgatg ggcgttgagt tcagcgaaga tatgctggat ttccctgcgc
     7681 tttttggccg tgaagcgccg gtgacgcttg agattggttt tggcatgggc gcgtcgttgg
     7741 tggcaatggc taaagatcgc cccgagcagg acttcctcgg cattgaagtg cattcaccgg
     7801 gcgttggtgc gtgcctgtct tctgcgcatg aagagggctt aagcaacctg cgcgtgatgt
     7861 gtcacgatgc ggttgaagtg ctgcataaaa tgattcctga caattcattg cgcatggtgc
     7921 agctcttttt ccctgacccg tggcacaaag cgcgccataa taaacgccgt atcgttcagg
     7981 tgccgtttgc cgagctggta aaaagcaaac tgcagctggg gggcatattc catatggcga
     8041 ccgactggga accttatgcg gaacatatgc ttgaagtgat gtcttctatt gacggttata
     8101 aaaacctgtc agagagcaat gattacgtac cgcgtccggc atcacgtccg gtgacgaaat
     8161 ttgaacaacg tggtcatcgt cttggtcacg gagtatggga cttaatgttc gagagggtga
     8221 aataatggca aagaaccgta gccgtcgtct gcgtaaaaaa atgcacatcg acgaattcca
     8281 ggaattagga ttttcggtgg catggcgatt cccggaaggt acatcggaag aacagattga
     8341 taaaaccgtt gatgacttta ttaacgaggt tatcgaaccg aacaaactgg cctttgacgg
     8401 cagcggttat ctggcctggg aaggtctgat ctgcatgcag gaaatcggca aatgcaccga
     8461 agaacatcag gcgattgtgc gtaagtggct ggaagaacgc aaactggaag aggtacgcac
     8521 cagcgaactt ttcgacgttt ggtgggacta agaaagcata cgggcgatga caaatgcaaa
     8581 actgcctgat gtgctacgct tatcaggcct ataaagatgc acgatcgagt aggctggata
     8641 aggcgtttac gccgcatccg gcatggaaaa cgcgcacttt gttatcaatc tggggccagc
     8701 aaatgctggc ctgatttgtt cttgagggaa gactatgatg cgcaaaatgc tgctggcggc
     8761 agcactttca gtgacggcaa tgaccgctca cgccgactac cagtgcagcg tcacgccgcg
     8821 tgacgatgtg attgtcagcc cgcaaaccgt gcaggtgaag ggcgaaaacg gcaatctggt
     8881 gatcacgcca gacggcaacg tgatgtataa cggtaagcaa tattccctga atgccgccca
     8941 gcgcgagcag gcgaaggatt atcaggctga actacgtagc accctgccgt ggattgatga
     9001 aggcgcgaaa agccgcgtcg agaaagcccg tattgcgctg gataaaatta tcgttcagga
     9061 gatgggcgaa agcagcaaaa tgcgcagccg tctgaccaaa cttgatgcgc agctgaaaga
     9121 gcagatgaac cgcattatcg aaacgcgcag cgatggcctg acgtttcact ataaagccat
     9181 tgatcaggtt cgcgccgaag gccagcaatt agtgaatcag gcaatgggcg gaattttaca
     9241 ggacagcatt aatgaaatgg gcgcgaaagc ggtgctgaaa agcggcggta acccattaca
     9301 gaacgtgctg ggaagcctgg gcggtctgca atcctcaatc caaaccgagt ggaaaaagca
     9361 ggaaaaagat ttccagcagt ttggcaaaga tgtttgtagc cgcgttgtga ctctggaaga
     9421 tagccgcaaa gccctggtcg ggaatttaaa ataatcctct attctaagac ggcataatac
     9481 ttttttatgc cgtttaattc ttcgttttgt tacctgcctc taactttgta gatctccaaa
     9541 atatattcat gttgtaaatt gtttaacgtc aagtttccca tacaaagcta agggataatg
     9601 cgtagcgttc acgtaactgg aggaatgaaa tggagttttt caaaaagacg gcacttgccg
     9661 cactggttat gggttttagt ggtgcagcat tggcattacc caatatcacc attttagcaa
     9721 ccggcgggac cattgccggt ggtggtgact ccgcaaccaa atctaactac acagcgggta
     9781 aagttggcgt agaaaatctg gttaatgcgg tgccgcaact gaaggacatt gcgaacgtta
     9841 aaggcgagca ggtagtgaat attggctccc aggacatgaa cgatgatgtc tggctgacac
     9901 tggcgaaaaa aattaacacc gactgcgata aaactgacgg cttcgtcatt acccacggta
     9961 ccgacacgat ggaagaaacc gcttacttcc tcgacctgac ggtgaaatgc gacaaaccgg
    10021 tggtgatggt cggtgcaatg cgcccgtcca cgtctatgag cgcagacggt ccattcaacc
    10081 tgtataacgc ggtagtgacc gcagctgata aagcctccgc taatcgtggc gtactggtag
    10141 tgatgaacga caccgtgctt gatggccgtg atgtcaccaa aaccaacacc accgacgtag
    10201 cgaccttcaa gtctgttaac tacggtcctc tgggttacat tcacaacggt aagattgact
    10261 accaacgtac cccggcacgt aagcacacca gcgacacgcc gttcgatgtc tctaagctga
    10321 atgaactgcc gaaagtcggc attgtttata actacgctaa cgcatccgat cttccggcta
    10381 aagcactggt agatgcgggc tatgatggca tcgttagcgc tggcgtgggt aacggcaacc
    10441 tgtataaaac cgtatttgac acccttgcaa ccgctgcgaa aaacggcact gcagtagtgc
    10501 gttcttcccg cgtaccgacg ggcgctacca ctcaggatgc cgaagtggat gatgcgaaat
    10561 acggcttcgt cgcctctggc acgttgaacc cgcaaaaagc gcgcgttctg ctgcaactgg
    10621 ctctgacgca aactaaagat ccgcagcaga tccagcagat cttcaatcag tactaatcac
    10681 ttcgccccgg tatcgtgccg gggcttattc acttcagact cacgtccatt gccaattttt
    10741 attaccctaa tgataatcac cggaataaat tattccgcgc gagggttttc gggtgaaaaa
    10801 gcaatggatt gttggtacgg cgctgcttat gttgatgact ggtaatgtcc gggcagatgg
    10861 tgaaccgcca actgaaaata ttttaaaaga tctattcaaa aagcagtatc acggcattct
    10921 caagcttgat gtcatcacct taaaaaatct tgatgctaag ggtaatcagg ccacctggtc
    10981 agcggaaggc gatgtctctt ccagtgacga tctctatacc tgggtcggtc agttggcaga
    11041 ttacgagctg ctcgaacaga cctggacgaa agataaaccg gtgaaattct cggcgatgtt
    11101 aaccagtaaa ggaacgcccg cgtctggctg gtcggtgaac ttttactctt ttcaggcggc
    11161 agccagcgat cgtgggcggg tggttgacga tatcaaaacg aataataaat atctgatcgt
    11221 gaatagcgaa gatttcaatt atcgctttag tcagcttgag tctgcgttga ataaccagaa
    11281 taactcgatt cctgcactga aaaaggatgt aaaagcgctt gataaacaaa tggtggcggc
    11341 gcagaaagca gcggatgcat attggggaaa agatgcaaac ggtaagcaaa tgacccgcga
    11401 agacgccttt aaaaaaattc accaacagcg tgatgatttt aataaacaga acgatagtga
    11461 ggcgttcgcg gttaaatatg acaaagaggt ttatcaaccg gcgattgcgg catgtcataa
    11521 acagagtgaa gagtgttatg aagtgccaat tcagcagaaa cgagattttg atatcaatga
    11581 acaacggcga cagacttttc tgcaatcaca aaaacttagc cgtaaattgc aggatgactg
    11641 gataacgctc gaaaaagggc aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa
    11701 aaaagtcacg attttgatga aaattgacga tatcaaccag gctaatgaac gctggaaaaa
    11761 agataccgaa caattgcggc gcaatggggt gattaagtaa tttttgcgat gacgcaagag
    11821 ttgtagccgg atgttgcgta cacgccgcat ccggcaatac aaatttactc agccagaaaa
    11881 agctccagca gcgaatttaa aaacaacttc ccatgttccg ttatctgcca gtaatccgca
    11941 cattctgtga gataaccctg agcgatagcc tcttctaact gtgggcgaat aacctcttct
    12001 gaaaggccag tatactggct aaactccacg cgcggcgcgg cttccagcaa acggaagcga
    12061 ttcataaaga actcaaatgg tttatctgcg gcttcgacat cacgctggct ttccagatac
    12121 cgcccctgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    12181 gggaaggtca ctttgccgtg cgcgccgcag ccaataccaa tgtagtcacc aaagcgccag
    12241 tagttgagat tgtgctggca ctgataaccg ggtttggagt aagcggaagt ttcatattgc
    12301 tgataacccg ctgcggttaa taactgatgc ccctgttcga atatatccca caacgcatcg
    12361 tcgtccggca gcaccggtgg gcgagagcca aacagcgtat taggttcgat ggtcagttga
    12421 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc tagcgcctct
    12481 tccagtgatt gatccggcag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    12541 ccgctcgcca gcttcgcagc tcgtttcgct tcttgcgggc catgaatgcg cccaagtcgt
    12601 ttcagctttt cttcgctaaa actctgcaca ccaatagaga tgcggttcac accagcacgc
    12661 tgataatcga caaagcgatc ggcttcaacc gtaccagggt tcgcttccat cgtaatttct
    12721 gcatccgctg tcagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccggg
    12781 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctgcacttc acggccctga
    12841 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacataatc gtcgtgcggc
    12901 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    12961 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    13021 cagtttcaac gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    13081 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    13141 cgctggttca cgagtaatca cgcctggcca gctaccgtgg cataccagcg gagtgggatc
    13201 ttccgcgtga cgcagatata ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    13261 tacgtctttc attgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    13321 ggaataacgc gcggagtaaa tacccggcgc gccacccagc gcatctaccg ccagaccgga
    13381 gtcgtcggca attgccggta aaccggtcac ttttgccgca tggcgcgctt tcagaatcgc
    13441 gttttcgata aaggtcaggc cggtttcttc agcggaatct acgccgaggt ctgtttgggc
    13501 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    13561 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgatttctg
    13621 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    13681 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    13741 tgttggcctg gccgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    13801 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    13861 catcattaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    13921 ggtcgcctgc aagacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    13981 catcggcgag aaatcaatcc cacccattgc cggtagcaag cggcgaatcg ggcgcagcag
    14041 cggatcggcc agctgaatca gcacgtattc aatcgggcta cgaccctggc ttacccagct
    14101 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    14161 caataaaccg gcaatccaaa tgattggcag gaaggtcacc actttaaaca gcacgatggc
    14221 tttaatgaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg ggcccattgc
    14281 cggaataacg cggcgcagtg gcccgataat cggctgcgtt accttcacta caaactgtga
    14341 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    14401 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    14461 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    14521 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    14581 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    14641 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    14701 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt cagccagttg aatcccggac
    14761 ttactgtttt catcactaat gttaatttga atcagtacgt taagaggggg aagttctgcc
    14821 gggcgctggt cgttcaggcg ggtagcgatg cgcaaacggt cgatggtatg acaccagtcg
    14881 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    14941 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    15001 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    15061 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc
    15121 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    15181 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    15241 acacctgtgc agcgcctggc ccgcacgatg gcgcatacgc ggcagaatgg aagctgcgcc
    15301 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    15361 aatattgctg gagaatggtc agctggattt tgctgtgtcg ctggcggaaa accagcgatt
    15421 gcgcggcagt gcgttcgcac aacggcaagg tatttctctg gcgttacggc tgttaccttc
    15481 gcactgcccg cagctcgaac agcttggcgc accaccggta ttgccggaat tactcaagag
    15541 cgagaatggc ctgattctgg tgacgggggc gacggggagc ggcaaatcta ccacgctggc
    15601 ggcgatggtt ggctatctca atcaacatgc cgatgcgcat attctgacgc tggaagatcc
    15661 tgtggaatat ctctatacca gtcagcgatg tttgatccag cagcgggaga ttggtttgca
    15721 ctgtatgact ttcacatcgg gattgcgggc tgcattgcgg gaagatcctg atgtgatttt
    15781 gctcggagag ctgcgtgata gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    15841 gcatctggtg ctggcgacat tacacacgcg cggcgcagcg caggcagttg agcgactggt
    15901 ggattcattt cctgcgcagg aaaaagatcc cgtgcgtaat caactggcag gtagtttacg
    15961 ggcagtgttg tcacaaaaac tggaagtgga taaacaggaa ggacgcgtgg cgctgtttga
    16021 attactgatt aacacacccg cggtggggaa tttgattcgt gaagggaaaa cccaccagtt
    16081 accgcatgtt attcaaaccg ggcagcaggt ggggatgata acgtttcagc agagttatca
    16141 gcagcgggtg aaagaagggc gcttgtgacg ttgctgtgtc tgatacgaat tgtaaatatg
    16201 cccgatgcgc aagcttatcg ggcctacaag ggaagcgtaa ctttgaattc gcactcactg
    16261 taggttggat aaggcgttta cgccgcatcc aacatgaaac aacgagcaca ttgtcagaaa
    16321 tctgtgcgct agtaaatcgc cttaatattc ctgctcgaaa tagctttcga gaataataac
    16381 cgcagaggca gagtcgactt tgcctttgtt gagcgcccga tagccgccct gttcaaacag
    16441 accggaacgt gcctccacgg tgctaagacg ctcgtcatgg agctttactt caacaccgaa
    16501 acgaccatga atacggttgg caaatttacg cgctctggcg gttaacggct gttcggtgcc
    16561 gtccatattc agcggcaaac cgacgatgat ttcgtccggc tgccactctt tcagtaaacg
    16621 ctcgataatg ttccagtccg gcgtaccgtc ctgcgcttta attgcaggca aagggcgagc
    16681 ggtgccggta atgcgttggc cgaccgctac gccaatgctt ttggtgccga agtcgaaggc
    16741 gagtaaggtt ccactcatca ggcgtgtcct gccacaccag gcatggtgag aatatccaca
    16801 ccaatcagtt ttgccgcctc gcgccagcgg tcggcaatcg gcgttttgaa cagaatattc
    16861 agatctgccg gggccgttag ccacgcgtta tcgagaattt cttgttccag ttgacctttc
    16921 tcccaggagg cataacccag agccaccaat acgtcagacg gttgtttatc ggtgccgagc
    16981 gtttccagca catcgcggga agtggtcatc actgtgttgt ctgaaatgcg aatgctggaa
    17041 gcaaaattgg agggcggagt atgcaaaata aacccgcgat cttcagccag cggaccgccg
    17101 agcataaccg gtttatccag acggattgat tcatcacgcg gctccggcgt gatcttcagc
    17161 ttttccagaa tcccttcaat tttgagattt tccagcggct tgttgacgat gatccccatt
    17221 gcaccattgg tattatgttc gcaaatgtag accacggaac gacggaaaat cggatcctgg
    17281 agagcaggca tggcaataag aaagtgatgc tgtaaattca ttgtcagagg ttctgtttcc
    17341 tggttcaaaa aagcaacagc gcccagtatg cgtggaaaga agggcgctgt cactcagagt
    17401 ctcaacgaga tccttctcgc taaggtgggt tactgctgct gtaaacgtgc ttcgatggca
    17461 tccattaaca ttccggtgat cgacaccgga aactctgctt caatctcacg aatacaggtt
    17521 gggctggtga cgttaatttc agtcagacgg tcgccgatga tatccagacc aacaaaaatc
    17581 agcccttttt ctttcagcgt cggcccgatc tgacgggcga ttttccagtc actttccgtc
    17641 agcggacgag gttcaccgcg accaccggca gccagattgc cacgggtttc gcccccctgc
    17701 ggaatacgcg ccaggcagta cggtaccggc tcgccatcta ccaccagcac gcgtttgtcg
    17761 ccatctttaa tggctggcag gtaattttgc gccatgcagt agcgagtgcc atgctcagtc
    17821 agggtttcgg caatcacgcc gaggtttgga tcgccttctt tcacgcggaa aatcgacgcg
    17881 ccgcccatac cgtccagtgg cttaagaatg atgtcgctgt gtttctccca gaacgctttc
    17941 agctgtgctt tattgcgcgt aaccagcgtt tctggcgtta agtcagagaa ccaggcggta
    18001 aacagtttct cgttacagtc gcgcaggctc tgcggcttat taacgatcag cgtccctttc
    18061 tcttcggcac gttccagaat ataggtcgcg tagataaact cggtatcaaa cggcgggtct
    18121 ttacgcatca ggatcacatc gagatcggcc agcggcagat cctgttcacc gacgaacgaa
    18181 aaccactctt cgtagttctg cttcacattc agcgtgcggg tatgggcgcg ggcttcgcca
    18241 ttgatcagat acagatcgcc catctctata tagtgaagtt cgtaaccacg acgctgtgct
    18301 tccagcaaca tagcgaaact ggaatctttc ttgatgttga tgtttgcgat ggggtccatc
    18361 acgatgccga gcttgatcat tattcttctc cgttagccca aatcgccaaa tcgtacttgt
    18421 agcgcggtaa tggcggtgag cgcagttgtc tctgtacgca aaacgcgagg tcccaacagg
    18481 atatcagtaa attgatagcg ggcagtcatg gcaatttcat ctgccgataa accgccttcc
    18541 gggccaatca gcaggcggac gcgttcaacc ggtaacggca acgtattgat gctgttactg
    18601 gcgcgcgggt gaagattcag tttcagtcct tcatcctgct ctgcacacca ggcttccaga
    18661 tccatcgctg gacggatttc cggcacccgg ttacgaccac actgctcaca ggcagcaatt
    18721 gcaatcttct gccactgctg gagcttcttg ttcagacgtt cactatccag tttaacgccg
    18781 cagcgctcag aaaaaagtgg cgtaatgagg cttacaccga gttcgatcga tttctggata
    18841 gtaaattcca ttttttcacc acgcgacatc acctgaccga ggtgaatatg cagcggagat
    18901 tcgcgatcgt cgatctggcc ttccagcacc ttcacttcca cgcttttttt gctggcgctg
    18961 gtaatttcgg cgtcaaagac ctggttgctg ccgtcaaaca attgcagcgc ctgtcccggc
    19021 cccatacgca gtacgcgccc gatatggttg gcggcatctt cgcaaagcgc gatgtgagaa
    19081 tggctggtca gtggttcagg atgataaatg cgggggatac gcatagttaa aaatccgcgt
    19141 cgtctcccca cgcggttgta cgcgtggggt aggggttaac aaaaagaatc ccgctagtgt
    19201 aggttagctc tttcgcgcct ggcaagcgcg ttgcacatac gggttatgat tgccctgcac
    19261 cttcgcgatg cgttcatcac gctcgcactc ccagtcggta actggataca tcttgtccca
    19321 tgcgttgaac agttgcgttt gctggcgaga gagtgtcagg ttgtattggt cacgcatata
    19381 gaagtaggtg cgcgcaatgg caccgcgtgc acgcgctggt ggttcggcaa ctttttcttt
    19441 gaaatcgacc ttcatggcgc attgaccgta ctggccttca ccgccattcc actggctgta
    19501 cataaagttg ccgcgatcgc cattcacctc accgactgac ggctgcaggt tatgcatatc
    19561 gctttccatc ttgcgataga ccggatcttt ggcgcaattt ttacgtccac cgtcctgcca
    19621 gcactggcgc tggtgaccga attgccaggc aggaacgacg tgttcccact ctacgcggct
    19681 ggcacggttt tcattttttc gcacctgata gccgcacgat tgcagatcaa caacgccttt
    19741 tttgccctgc cagttaattt tacatccgca ataaaacgta ccgggcgcgt cagcgtggac
    19801 ttttaccgcc gcggctttcg cttgagaaaa actattgata ccttcggcca atgccgggcc
    19861 ggaaaatgct gcgctcagta ccaccgcagc aatagacaaa taacggtaca tcacttactc
    19921 cgtgttaaaa cgagccagca acgtagcgaa agttgttttg gtatgcaatc agccagatca
    19981 ggaaagttcc tgatagttca ggtggttatt tcgcaaccag ctgttcgccg cagtgaacac
    20041 agcgatagac ggcttcgcca cgaactacgc gattatgacg gcgtacggta agctgatgct
    20101 cctggcactt gcagcggtag gggaaggtgt tgcggcgcac ggattgcagt tcgaactgat
    20161 gcgtacggcg ggcgggaacg cccagcacgc tttccatcat ccacttccac tctttgccat
    20221 gtggcgctac gcggccgaaa tgtttccata ccagcaaatg tgccagttcg tgcggcacca
    20281 cttcttcaat aaaggcttca ctgttttcca acagcaaaac gggattgagg cgaatttcat
    20341 agctttccag ccaggctgtt ccggcagagg ttccgcgctg ggtgtaagag agttttggct
    20401 ccgggtagtt acgccctagc ttcaggttgg cctgggcgag tttttcccgc aggcgacgca
    20461 taacggcctg ttggatggcg atagggagac gggatgtttt catagcggca gaggatagag
    20521 cgaagaaaag atgactgcaa gaggtggctt cctccatgat gggaggaagc ctgaggagat
    20581 taatcgtgag cgccgatttc gcgcagttta cgacctttca tcagattacg ttcaatatgt
    20641 tccagcgaaa cgtgtttggt ttccggtacc agccacaatg tcagcaggat aaacagtacg
    20701 ttcagagcag catacaccca gaaggtgttg gcgttaccca gcgtgttgag catggtcagg
    20761 aacgttgcgc caacgatcat gttggcaatc cagttggtgg cagtggagca ggtgatgcca
    20821 aaatcgcggc ctttcagcgg ctgaatttcg gagcacagta cccaaatcag cggaccggca
    20881 ctcatggcaa aaccgacaat aaacatcagc agcatggcga tggcgaaata ctgcgccgac
    20941 ggagagtgga taccgatatg catcattgta ccgagtacgc ccataccagc agccatcacc
    21001 aggaagccca gcgttagcgt tggtttacgt ccccagcggt caacaaggcc gattgcgata
    21061 aaggtggcaa gtacgttggt caggccgaca atcactgtcc cccacatttg ctcggtggtg
    21121 ttggtataac ccgccagttc gaagattttc ggcgcgtaat acatgatgac gttcatcccg
    21181 gtgaattgct gcattatctg caatagtacg ccaaggaaca ccgcgcggcg gaagttgctg
    21241 ttctctttaa acagcgccca gccactctgt ttaacctgca aactttcacg gatttcatcc
    21301 agttcgcgtt tcgcttccgc gctggtgtca cgcaggcgta gcagcacgcg ttcggcatca
    21361 acaaaacggc gtttggcggc aaaccaacgt gggctgtctg gcaggaagaa gacaccaatc
    21421 agcagcaaaa ttgccgggat gataatcaca cccagcatcc agcgccatgc accggtgtag
    21481 ctgaaggcgg tatcagaaag ataagcgccg aggatcccga tagtgatcat caactgatac
    21541 atcgagatca tactgccgcg aattttttcc ggcgcaattt cagagaggta cagcggtgcg
    21601 gtataagagg ccacacccac tgccagcccc agcaaaacgc gggaaagaat cagtacttca
    21661 acgtttggcg cagccgcaga gaacagtgaa ccggcaacaa acaagattgc gccgatcatc
    21721 aggctctttt tgcgcccgag tttaaaggag agccagccgc tgcccaccgc accgactgcc
    21781 gcaccgaaca tcatggagct tacgacccat tcttgcgtgt gcgaagtaat ctggaattca
    21841 tctgcaataa acggcagtgc gccagcaatt acaccgatat ccaggccaaa gagtaatccc
    21901 gccagagcgg caaggaagca gacgaaaaac gtcattgcct tgtttgaccg cccctgtttt
    21961 ttagcgtcag gcatgatgcc ctccaatatg gttatttttt attgtgaatt aagataggtg
    22021 agtacgacgt aaaaagatgt gaagcaaatc acatcagtgt aatcgcttac actaacattt
    22081 atcttagtta tgatttaagt gcatgaaa
//
LOCUS       DADTFG010000081        22015 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.081, whole genome shotgun sequence.
ACCESSION   DADTFG010000081 DADTFG010000000
VERSION     DADTFG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.081"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            55..1731
                     /locus_tag="J1138_002809"
     CDS             55..1731
                     /locus_tag="J1138_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBA8454579.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(1861..3165)
                     /locus_tag="J1138_002810"
     CDS             complement(1861..3165)
                     /locus_tag="J1138_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBA8454580.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            3317..4276
                     /gene="aes"
                     /locus_tag="J1138_002811"
     CDS             3317..4276
                     /gene="aes"
                     /locus_tag="J1138_002811"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBA8454581.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(4273..5235)
                     /gene="hemH"
                     /locus_tag="J1138_002812"
     CDS             complement(4273..5235)
                     /gene="hemH"
                     /locus_tag="J1138_002812"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBA8454582.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATSEHIEMMANLVAAYR"
     gene            complement(5367..6011)
                     /gene="adk"
                     /locus_tag="J1138_002813"
     CDS             complement(5367..6011)
                     /gene="adk"
                     /locus_tag="J1138_002813"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBA8454583.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            complement(6192..8066)
                     /gene="htpG"
                     /locus_tag="J1138_002814"
     CDS             complement(6192..8066)
                     /gene="htpG"
                     /locus_tag="J1138_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBA8454584.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(8176..8781)
                     /gene="recR"
                     /locus_tag="J1138_002815"
     CDS             complement(8176..8781)
                     /gene="recR"
                     /locus_tag="J1138_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBA8454585.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRKENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(8781..9110)
                     /locus_tag="J1138_002816"
     CDS             complement(8781..9110)
                     /locus_tag="J1138_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBA8454586.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(9163..11094)
                     /gene="dnaX"
                     /locus_tag="J1138_002817"
     CDS             complement(9163..11094)
                     /gene="dnaX"
                     /locus_tag="J1138_002817"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBA8454587.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     gene            complement(11223..11774)
                     /gene="apt"
                     /locus_tag="J1138_002818"
     CDS             complement(11223..11774)
                     /gene="apt"
                     /locus_tag="J1138_002818"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBA8454588.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(11927..12304)
                     /locus_tag="J1138_002819"
     CDS             complement(11927..12304)
                     /locus_tag="J1138_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBA8454589.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12374..12901
                     /gene="priC"
                     /locus_tag="J1138_002820"
     CDS             12374..12901
                     /gene="priC"
                     /locus_tag="J1138_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBA8454590.1"
                     /translation="MKTTLLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12915..13076
                     /gene="rsmS"
                     /locus_tag="J1138_002821"
     CDS             12915..13076
                     /gene="rsmS"
                     /locus_tag="J1138_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBA8454591.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(13288..16650)
                     /gene="mscK"
                     /locus_tag="J1138_002822"
     CDS             complement(13288..16650)
                     /gene="mscK"
                     /locus_tag="J1138_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBA8454592.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(16778..17425)
                     /gene="acrR"
                     /locus_tag="J1138_002823"
     CDS             complement(16778..17425)
                     /gene="acrR"
                     /locus_tag="J1138_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBA8454593.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            17567..18760
                     /gene="acrA"
                     /locus_tag="J1138_002824"
     CDS             17567..18760
                     /gene="acrA"
                     /locus_tag="J1138_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBA8454594.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETSRINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            18783..21932
                     /locus_tag="J1138_002825"
     CDS             18783..21932
                     /locus_tag="J1138_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBA8454595.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         5053 a         5787 c         5847 g         5328 t
ORIGIN      
        1 tttttggttt tcccggccaa aaatggacaa taaccaccag gaaaaggaga cggaatgcat
       61 cacgccaccc cgcttatcac caccattgtt ggcggccttg tgctcgcctt tatcctcggc
      121 atgctggcca ataaactacg tatttctcct ctggtgggat atctgttagc gggtgtgctg
      181 gcaggaccat tcactccggg ctttgttgcc gataccaagc ttgcgccgga actggctgaa
      241 ctgggcgtta ttttgctgat gttcggcgtc ggtttgcatt tttcgctcaa ggatttgatg
      301 gcggtaaagt ccatcgccat tcccggcgcg atcgcccaga tagccgtggc gacgctgctg
      361 ggtatggcgc tctccgccgt gctgggctgg tcgttaatga ccggtatcgt gttcggctta
      421 tgtctttcca ccgccagtac cgtggtgtta ctgcgcgcac ttgaagaacg gcaattaatt
      481 gacagtcagc gtgggcaaat cgccatcggt tggttgattg tggaagacct ggtgatggtc
      541 ctgacactgg tattgctacc cgcggtagca ggaatgatgg aacagggcga tgtgggcttt
      601 gccactcttg cagtcgatat ggggatcacc atcggcaagg tgatcgcatt tatcgccatt
      661 atgatgctgg taggtcgccg tctggtgccg tggattatgg cacgcagcgc agcaaccggt
      721 tcccgcgagc tgtttaccct gtcggtgctg gcgctggcgt tagggattgc ctttggtgcg
      781 gtagagctgt ttgatgtctc ctttgcactc ggtgcgttct ttgccgggat ggtactgaac
      841 gagtctgaac tgagtcaccg tgccgcccac gatacactgc cattgcgcga cgcgtttgcg
      901 gtgctgtttt ttgtctccgt cgggatgttg tttgatccgt taattctgat tcagcaaccg
      961 ctggcagtac tggcgacgct ggcaattatt ctgtttggta aatcggtcgc ggccctgttc
     1021 cttgtgcgtt tgtttggtca ctcccagcgc acggcgttaa ccatcgccgc cagcctggcg
     1081 cagattggtg agttcgcgtt tatcctggcg ggactaggaa tggcattgaa tttactgccg
     1141 caggccggac aaaacctggt actggcaggg gcgatcctgt cgattatgct caacccggta
     1201 ctgttcgcac tactggagaa atatctggcg aagaccgaaa cgctggaaga gcagacgctg
     1261 gaagaggcaa tcgaagaaga gaagcagatc ccagtggata tttgcaacca tgcgctactg
     1321 gtgggttacg gtcgtgtagg cagcctgctg ggggagaaat tgctcgcctc tgatattccg
     1381 ctggtggtga ttgagacgtc acgaacccgt gttgatgagc tgcgcgagcg cggggtccgc
     1441 gcagtattgg gcaatgcggc gaacgaagaa attatgcaac tggcgcatct ggaatgtgca
     1501 aaatggctga tcctgacgat tcccaacggc tatgaagcgg gcgagattgt ggcatctgcc
     1561 cgcgcgaaaa atccggatat tgagattatt gcccgcgccc attatgacga tgaagtggcg
     1621 tatatcaccg aacgtggtgc gaatcaggta gtgatgggtg agcgtgaaat tgcccgtact
     1681 atgctggaac tgctggaaac gccaccggcg ggtgaggtgg tgacggggta aacggtggtt
     1741 gccggataat aggcgcggat atgtaggcct gataagacgc gccagcgtcg catcaggcat
     1801 tgaaagcaaa atgccagatg cggcgtgaac gccttatctg gcctacgaac cgacgataac
     1861 ttaacgatcc cagtaagact cttccaggct gtcttcacgc tccggcaagc cgcgcgttaa
     1921 acgaggtgaa tgctggttca gtacctgata gctcacacgg ttagcatatt tacacacctg
     1981 cgctaacgat gaataagtta accaggtgaa tttatgtttg ctggagtttg gtacgttgct
     2041 acggtggtag ctgttggcgg taatgtcatg cagcaacgct gccaatgcgc catcccctgc
     2101 tccattagtg ttcatgattt tttccggccc gcccatgtac ggcgcaatgt gcgaatagac
     2161 acgcagcgga ttctggcaat ccttgtggcg catggcgcgg ctaaactcat actggttaaa
     2221 ttccgctata gcacccggca gcagcggatg ctgggtttta cgtttcgctt cgtcttcggt
     2281 aaagcccgcc atatacaagc cgattggccc ggcggtgcac agcaccagat ctacccagtc
     2341 cagcgccttg tcagatgcca acaacggatc gctttctccg gtcaacgctt cggcttcatc
     2401 ttcgttcatc gcaaggatag agacgtggtc tttgaggaat tgctgccacc actgcggatt
     2461 ctcggcaatg acaaacttgg tgcccagcgt cagcaccacc ggtacgttat atttcttcgc
     2521 gtactcaatg gctttcatag ttgcttccgg catgggttca cccggcttgc aacgcaccag
     2581 ataagaggtg agaaccagag ccgaggctcc ggcaatcaca tcttccggaa tactttcagc
     2641 ccgcagctgg ttcatgtggc cggggctgat agcaaaggta cgttccccgg actcgccaat
     2701 cagcgtaaag caacgaccaa ttgggccatc cacgccttgt agatagttaa gatcggtacg
     2761 gctggaggtg ttacacaggt aacgataggc atagctgcca atttcaatat tgctgcacat
     2821 gacgcccagc agcaccgaac ggtcatccgc gagcaccgag tagttgtgca tggtgttacc
     2881 aatggtgcca cccgcaaact gatgggtaat caggtttttc tgttttagtt cctgataaag
     2941 cgcttcggca acgtcatcct caatcaccaa tgaatgcccg gcgcttaatc cataacgctc
     3001 aatgaattca tcatccactt tcgcttcaat atcgaccagc gtttgatcga tacccactac
     3061 ccaggcggcg ctggtttcgt tttctggctg gaattgctga agcagcggat cgcgtgcgtt
     3121 tacggggaag taatgtttgg atttacgttt accgggaaat ttcatggttg tttttacggg
     3181 tggttaactg agcggcgaat gttaacacaa tctgacggga gcgcgcgaga cacagccagt
     3241 ttcaatatca gcaggcggga tcgcattttc atggcagtgg ttccttacaa tgacgtaatt
     3301 tgaaaggagt ttttgcatga agccggaaaa caaattaccc gttctggacc ttatctctgc
     3361 tgaaatgaag accgttgtga atactcttca gccggattta ccgccctggc ctgcaacggg
     3421 aacgattgct gagcaacgac agtattacac gcttgagcgc cgattctgga atgtgggcgc
     3481 tccagaaatg gcaaccagag cttacagggt tccaacaaaa tatgggcagg tgaaaacacg
     3541 tatcttttat ccgcagccag atagcccggc gacgctattt tatttgcatg gaggcggttt
     3601 tattctcggc aatctcgata cccacgatcg catcatgcgc ctgctggcaa gctacagcca
     3661 atgtacggtg attggcattg attacaccct ttcacctgaa gcgcgttttc cgcaagcgat
     3721 agaggaaatt gtggctgcct gttgttattt ccaccagcag gcggaggatt atcaaatcaa
     3781 tatgtcccgc attggttttg ccggtgattc cgcaggtgcc atgctggcgc tcgccagtgc
     3841 gttgtggttg cgcgataaac agatcgattg cggtaaagtt gcgggcgttt tgctgtggta
     3901 tgggctttac ggattacggg attccgtgac tcgtcgtctg ttgggtggtg tctgggatgg
     3961 cttaacgcaa caggatttgc agatgtatga agaggcgtat ttaagcaacg acgcggaccg
     4021 cgagtcgcca tattactgcc tgtttaataa tgatctcact cgcgaagttc cgccctgttt
     4081 tattgccggg gcggagttcg atccactgct ggatgacagc cgtctgcttt accagacgtt
     4141 agcggcgcat cagcagccct gtgagttcaa actctaccca ggcacgctgc acgccttttt
     4201 gcattattca cggatgatga aaaccgccga cgaggctctt cgcgacggcg ctcagttctt
     4261 taccgctcag ctttagcgat acgcggcaac aagattagcc atcatttcga tatgttccga
     4321 cgtggcatta agcgccggaa tatattcata ttttttcccg cccgcaccga ggaagacctc
     4381 acggttttgc tcggcaatct cttccagcgt ctccagacaa tccgcagcaa agcccgggca
     4441 catcacctga atatgaccca cgcctttttc tccgagcatt ttcagcgttt cgtcggtata
     4501 aggcatcagc cagggttccc ggccaaagcg cgactgaaag gtcatcatca ctttttccgg
     4561 tgccatctcc agtgcggaag cgagttcgcg agttgttgtg cggcaacgtt gcgggtaatc
     4621 atcgccttca tctgcataac gctggggaat gccatgataa gagagcaaca gcagatccgg
     4681 ttcgccatgt ttggcaaaag aagcacgtac gctgttcgcc agtgcattaa tataatcgtg
     4741 gttatcggcg taatcacgaa taaacgatat ccccggaatg ctacgtttgc gcgccagaat
     4801 tcgcgccagt tcatcccata ccgcaccgac cgttgaacag gagtattgcg gataaagcgg
     4861 cagcaccaca atatgatcta catgctctgc cagaagttca tctacggcgc tttccagtga
     4921 tggcgagccg tagctcattc ccagcgccac gggcgtctcc ggtaaacgtt gcgccagcgc
     4981 ctgctgttgc tggcggctgt aaaccatcag cggcgagcca ccttccatcc agacagaggc
     5041 atacagcttc gccacacgcg gcgaacgcag cggcaaaatc acgccgcgca gcaacggcca
     5101 ccataacaac cgtgaggtat caaccacgcg tctgtcgctt aaaaattgtt tcagatagcg
     5161 ttttaccgct tcaggtgtgg gggcatcagg cgtacccagg tttgccagca ggataccggt
     5221 tttagtttga cgcattaccg cctcttatcg attcaacttg ttgataattg tagcggaaaa
     5281 gtgaagaaga agaactaatt gctgtaagag gtattgcctg aggaggatga gcagacctgc
     5341 ccgagagcag atctgcggcg gcttgattag ccgagaattt tttccagagc tgcgcgaact
     5401 tcagccaccg gcttggtgcc gtcaactttc gcgtatttgg tgttacccgc ttccgcttct
     5461 ttggagtagt agccgatcag cggtgcagtc atctgatggt attcaaccag acgtttacgt
     5521 acggtctctt cctgatcgtc tttacgggta gtcagctctt caccggtaac gtcgtctttg
     5581 ccttctactt tcggcggatt gaatttaacg tgataaacac gaccagacgg cgcgtgaacg
     5641 cggcggccta cgatacggtc aacaatcagt tcgtccggta cgtcgaattc cagaacgtaa
     5701 tcaacattga tgcccgcttc tttcatcgcg tctgcctgcg gaatggtacg cgggaagccg
     5761 tccaacagga aaccgttgcg gcagtcttcc tgagcaatgc gctctttaac cagcgcgatc
     5821 accagttcgt cggtgaccag tttgccagca tccataatgt cttttgcttg tttacccagc
     5881 tcggagccag atttgaccgc agcacgcagc atatcgccag tggagatttg cggaatacca
     5941 tatttctcca tgatgaactg agcctgagtc cctttccccg cgcccggagc gccaagcaga
     6001 atgatacgca ttgcgaaaat ccccttaaaa tgtgtcgaat tttttgaaaa agcgataaac
     6061 gataccacca acaggcgatt gcctcaagaa aggcacctga ggctgaaacg gttaatggca
     6121 agataatttg cggaaagtgc agatgaagaa aagaaaaatg ccggatgaca cgaaggtcat
     6181 ccggcatgac atcaggaaac cagcagctgg ttcatacgac gaataaacag gttcggatct
     6241 tccagcgtac cgcgttctgc cagcagcgcc tgatccagca gcagttctac ccactcgctg
     6301 aacttcgctt catcttcagt atccgccgca cgtttcacca gtacgtgatc cgggttcagt
     6361 tcgaagatgt atttcacttc tggcactttc tggcccgccg cagcgaacag tttcgccatc
     6421 tgagtgctca tttcgtccgc gtcggtcgaa acgatcgctg gcgtatcggt cagacggtga
     6481 gtcagacgga catctttcac gcgctcgccg agcagggctt tcacacggtc gatgaacgga
     6541 gtcagtgctt tctccgcttc tttcgcgctc tcatcaactt cgtcagccag tttttccagc
     6601 gactcgtcaa ctttagacac agactggaac ggtttaccgt cgaactcagt cagatagttc
     6661 atcatccact catcgatgcg gtcggaaagc agcagaactt cgatgccttt cttacgcagc
     6721 agttccaggt gcgggctgct cttcgctgcc gcgtaactgt ctgcggtgat atagtagatt
     6781 ttctcctgcc cttctttcat gcgggaaacg tagtcttcca gagacacggt ctgcgcagaa
     6841 gaatcggtat gggtagaagc aaaacgcagc agtttggcga tcgcttcctg gttagcgaaa
     6901 tcttccgccg gaccttcttt cagtaccagg ccaaactgtt gccagaaggt ctggtatttt
     6961 tccgcgtcgt ctttcgccag tttttccagc atttgcagca cacgcttggt cagcgcattg
     7021 cgcaggttac gcgttaccgt gctgtcctgg aggatttcac gggaaacgtt cagcggcaga
     7081 tcgctggagt caatcagacc acgcacgaag cgcagatagt tcggcatgaa ctgttctgcg
     7141 tcgtccatga tgaacacacg ctgaacatac agtttcagac cgtgtttatg atcgcggttc
     7201 cacatatccc acggtgcctg agacggaatg tacagcaggc tggtgtactc ctgcttacct
     7261 tcaacgcggt tgtggctcca ggtcagcgga tcgttaaagt cgtgggcgat gtgtttgtag
     7321 aactctttgt actcttcatc ggtgatttcc gacttgttac gagtccacag cgcctgcgct
     7381 ttgttgattt tctcccagga gataatggtt tcgccgtctt tctcttcgcg tttttcgatc
     7441 tctaccggca gcgcgatatg gtcggaatat ttgctgatga tggaacgcac acgccagtca
     7501 tcgaggaact cgtcttcgcc ttcacgcaga tgcagggtga tttcagtacc acgatcttct
     7561 ttggtgatgt cggcaacagt gtattcacct tcgccagccg attcccagaa gacgccgttt
     7621 tctggttttt cgcctgccgc gcgagtacgc acggtcactt tgtcagccac gataaacgca
     7681 gagtagaaac caacaccaaa ctgaccgatc agctggctgt ctttcgcctg gtcagaaccc
     7741 aaggattcga ggaatgattt ggtgccggat ttagcgatag tccccagatg gtcaatcact
     7801 tcgtcgcggg tcatccccac gccgttatcg gagatggtca gcgtgcgctt gtctttatcg
     7861 aaagagacac gaacgcgtag ttcaccatca ccttcgtaca ggtccgggtt agaaagcgcg
     7921 cggaaacgca gcttgtccgc cgcatcggag gcgttagaga taagctcacg caggaagatt
     7981 tctttattgg aatagagaga atggatcatc aggtgcagaa gctgtttcac ttctgactga
     8041 aaaccacgag tttcttgtcc tttcatgtag gtctacctca ataatgccat tttaaggtta
     8101 aaaacaggat gagggagaga tggggacaag ggagagtaat ttcaagcgag agcaggtgat
     8161 cctgctctcg tttgcttaaa aacgaatctt atgacgcccg gcaagggagt gtgacagcgt
     8221 ggtgccatcg accatttcca gttcgccgcc aaccggtacg ccatgagcga ttcggctggc
     8281 ttccacgtca tattgcgcac aaagctcggc aatgtagtta gcggtagctt caccttcaac
     8341 cgtggggttg gtggcgagga tcacttcagt gattttttcc tctgccagac gctgttccag
     8401 acgatcaagc ccgatatcat ccggaccgat gccgtccagc ggtgacaggt gccccatcaa
     8461 cacaaaataa cggcctgaaa actgccccgt ctgctcaatg gcgtagatgt ccgccggact
     8521 ctccaccacg cagatttgac cgttttcctt acgacgcgga ttcgaacaga tgttacagac
     8581 ttcctgttcg gtgaaggtgc ggcaatcggc gcagtggccg atttccgaca tcgcccgggt
     8641 gagcgcctgc gccagacgca tcccgccgct acgatcgcgc tgaagcagcg tgaacgccat
     8701 acgctgcgcc gacttcgggc caacgcccgg cagacagcgc agtgcttcca taagctgtgt
     8761 taacagcggg ctggtttgca tcagaacggc atcttaaagc caggcggcag ctgcattccg
     8821 gaggatacag aggccatttt ttctttctgc gtttcttcaa tacgacgtgc tgcgtcgttg
     8881 aatgctgcag ccaccaggtc ttccagcatc tctttgtcgt cttccagcag gctcgggtcg
     8941 atctctacgc gacggcagtt gtgtgcaccg ttgatggtca cttttaccag acctgcgcca
     9001 gattcgccgg tgacttccag ctgcgcgatc tcttcctgca ttttctgcat tttttcttgc
     9061 atctgctggg cttgcttcat caggttaccc agaccgcctt taccaaacat aggtttctct
     9121 ctcaatcacg ttaaggatga cgaacgtaag ctgtgcttac gatcaaatgg ggcggatact
     9181 ttcttcatcc agctccgcat cgaagaatcg acgcagggtc tgaatattat tatccgcaat
     9241 aatggactcg cgcgcctgcg caagtttttc ttcgtatatc gcctgacgcc attccagcgg
     9301 cgtacgcacc gcgggattat catcttcaac gatagtcagt tcaaccgttg aaccttttaa
     9361 cgtgctcaac gcttcagcca gtttttgctg tgcgccgcgg ttgttcaaat gccgctgaga
     9421 ggagcgcaaa tgcagacata ctgcgttgtc gctctcctct ttccaggcat ttaacgccac
     9481 ctgttcgacc agttttggta gcgaaagttg actcacctga gccgcccacg ggtcgcgctc
     9541 aatggcttct gccgccagct tcgccgccag ttccggcgtt ttttcatgtt ccagcgcttt
     9601 tttcagcgcc ttcggcgtgg cgaccacttc tttttgctgc atcactggag tggtcgcctt
     9661 ccagcgatac gcttcttttt tggctggcgc tttttccagc gccgatggca ccgggcgcgc
     9721 ctgaacgcga tcggtgaccg aagccagtct ttccagcgca gcgttattca ccggccgcgc
     9781 gcgggtagcg gctgccggtt cactcttttt tgctttggtt gctccctgca cgcgctgcaa
     9841 ctgctggcgc gccgccagca cctggctggt ggtttccggg agcggtacag tcggtgcctg
     9901 ctgcggcgct gattgcggtt gcggcggcac ctgtgttggc gtcattactg ccgttggcgc
     9961 aacgggcgca aaggactggc gtggcacttc tggctcaggc agaggcatac gcggatggaa
    10021 cgccagcgcg cgcagcagcg tcatctcaac acccatacgg cggtctggcg catacggtaa
    10081 ttctttgcga ccaatcaaca gcgtctgata gtaaagctga atatccgtcg gcggtatggt
    10141 gcgcgccagt tcacgcatcc gcagctcaat ggcggccatg tcgttgccaa gagcagcagg
    10201 cgaaagttgt accatcgcga tacgatgcaa cagaccaagc atttccacca gcaacgcttc
    10261 ccactcgata ccacgggcag cagcttcatt aattagcgcc attacgcgct cgccgttggc
    10321 ctcgaccatc gcttcaacca gcgataacgc ctgatcgtcg tcaagcgtac ccagcatcgc
    10381 actgaccgcc tgggttgaaa cctggccgtc accgctggca atcgcctggt cggtcagact
    10441 taaggcatct cgcaggctgc cttcagcggc gcgtgccaac aattgcagcg cccgcggctc
    10501 gtgagcgata tgttcttcgt tgaggatgtg ctcaagctga tggcgaattt gctcgacatc
    10561 cagcgccttg agatgaaatt gcagacagcg tgacaaaatc gtcaccggca atttctgcgg
    10621 atcggtcgtc gccagcaaaa acttaacgtg ctctggcggc tcttcaaggg tttttaacag
    10681 tgcgttaaag ctgtgacgcg acagcatatg cacttcgtcg atgagataaa ctttgaaacg
    10741 accacgcgcc ggagcgtact gaacgttatc cagcaggtcg cgggtatctt cgactttagt
    10801 gcgcgaggcg gcgtcgattt caatcagatc gacaaagcgc ccctgctcga tttcacgaca
    10861 gttatcgcac acgccgcacg gcgtcgcggt aatgccggtt tcgcagttta gccccttcgc
    10921 caacagtcgg gcgatagagg tttttccgac gccacgggtg ccggaaaaaa gataagcatg
    10981 atgaatacgc cctaacgaca agccgttcgc cagtgcggtc agcacatgtt cctggccgac
    11041 gacgtcagca aaggtttgtg ggcgccattt tcgggctaag acctgataac tcattggcag
    11101 gctctgaaac gctggaaggt ggattcacga aggggtaatg ctaacacagc cccgtcagaa
    11161 cggcgaggct gaatgcacat cttgtgctgc ccgtagcgtg agcagcgcaa gactgacgat
    11221 aattaatggc ccgggaacgg gacaaggctg tagctggtaa tgccctgttt ttcgagacgc
    11281 tgttcgccgc cgagatcgaa caggttgata atgaacgcag cgtcagctac ttcaccaccc
    11341 agacgacgga tcagtttaac ggtcgcttcg atagtgccgc cggttgccag cagatcgtcc
    11401 accaccagaa ctttgtcgcc cggtttgatg gcatcaacgt ggatttccag ctgatcggtg
    11461 ccgtattcca ggtcgtaagt ttcactgatg gtttcacgcg gcagtttgcc cggtttacgg
    11521 accggtacaa agccaacgcc cagacccaga gctaccggag cgccaaacaa gaagccacgc
    11581 gcttcggtgc cgaccacttt ggtaatgccc gcatttttgt aacgctcaac cagcaagtcg
    11641 atgctgagag cgtaagcttt cgggtcttcc agtaagctgg tgacatcgcg gaaaagaatg
    11701 ccgggttttg ggtagtcctg aatgcttttg atgctatttt tgagatactc aagctgctgt
    11761 gcagtcgcgg tcataagtgt atgcctgctt gttacggtgg tactcacggc gcgttttgaa
    11821 cgtatcaaaa gtaacagttg tttaaccttt gaccgcctgc gcctgtgctc gaaaacgcca
    11881 gaatgtactg gctgtacgca ataattgcaa ctgcgattgt tgtgcttcag tgcttttctt
    11941 gcttttcatc aatcaccgga attcgccaca tataaaaaag caaacaggcg agaattacca
    12001 gcaacatgat gcgcacccac ggcatctgga cgaaccacag cgaaatcgta aacgtaatca
    12061 aaataagcaa aatcgcccgt ggttttgcgc cgcgcggcat cgcatgatgt ttctgccaga
    12121 aacgtagata actgccaaac catgagcggt acagcaacca ggcgtgaaag cgcggggaag
    12181 aacgggcaaa gcaccaggcc gccagcagga taaacggcgt cgtcggtaat accggtaata
    12241 ccacgcccag cgtacccagc actaccgcca gccagccaat gatgattaaa atgattcgtt
    12301 gcatattgag tgttgtcagc ttacagagtg gctactttag cataacaatt atcattttca
    12361 ttgaggtctt atcgtgaaaa ccaccctgct actggaaaaa ctggaaggac agctcgccac
    12421 gctgcgtcag cgttgtgccc cggtagcgca atttgccacg ctaagtgccc gttttgacag
    12481 gcaccttttt cagacccgtg cgacaacact acaggcttgt ctcgatgagg cgggcgataa
    12541 cctggctgcg cttcgtcatg cggttgagca gcaacagctg ccgcaagtcg cctggctggc
    12601 agaacatctg gcggcgcaat tggaggctat agcgcgtgaa gccacagcct ggtcgctacg
    12661 cgagtgggat agcgcaccac cgaaaattgc ccgctggcag cgtaaacgta ttcagcatca
    12721 ggattttgag cggcggctac gtgagatggt tgccgaacgt agagcccgtc tggcacgagc
    12781 gaccgatctc gtggaacagc agacgctgca tcgtgaagtg gaagcctatg aagcgcgcct
    12841 ggcacgctgc cgccatgcgc tggaaaaaat cgaaaacagg ttagcgcgtt taacccgcta
    12901 gcaatggaga gagtatgtca ctggaaaatg cccctgacga tgtcaaactg gccgtcgatt
    12961 tgattgtgct actggaagaa aatcagatcc cagcccgcac cgtgctgcgc gcgctggata
    13021 ttgtaaaacg cgattatgaa aagaaattaa cgcgcgatga tgaggcggaa aagtgagaaa
    13081 taaatggggc cgctaaatgt cccctaagat tgtgggcaaa cgcaaaactg cctgatgcgc
    13141 tacgcttatc aggcctacac cgctcctgca atatattgaa ttcttgcggt ttcgtaggcc
    13201 ggataaggcg gtcacgccgc atccggcatg aacaacgcgc actttgttag taatctcggg
    13261 gcaactaaat gtcgccccct ttggttatta cccaaccgct ggtgtcggat cgtcgccttt
    13321 gtagtcgcgt ttgacttccg tcacctcatc gcccttctcg ttgtgcagat gcacttcaag
    13381 ctggttaaag gcaatgttga tgtcgttttc acggcacaac tgatcgatag tacggttcag
    13441 ctcatcgaca gtacggctac ggtcacgcag ttcacgtaca tacagacgca gctcatgatc
    13501 caacgtgctg gcaccaaatg ccgtaaagaa gacttccggc attggttcgt gcatcaccct
    13561 tgggtgctca gtcgccgcct tcagcaacac tttacgcact ttttccagat cggagccata
    13621 ggctacgccg agacggatca ccagacgcgt agtcgtgtca gtcaacgacc agttgatcag
    13681 acgctcggta acaaacgctt tgttcgggat aatcacttct ttgcgatcga aatcggtaat
    13741 cgttgtcgca cgaatacgga tcttgctgac cgtacccgag aagctaccaa tggttaccgt
    13801 gtcgcctatg cgcaccggac gttcgaacag aatgatcaaa ccggaaacga agttaccgaa
    13861 gatctcttgt aaaccaaaac caagacctac ggataatgct gccgccagcc actggagttt
    13921 atcccacgag acgcccagcg atccgaacac cgtcatcgca ccaacggcaa taatgatgta
    13981 gttaaggatg gtagtaatgg catacgacgc gccctggcgc atattcagtc gcgagagcac
    14041 cagcacttcc agcaaaccag gcaggttgcg aatcaacgcc caggccacca ttgaggcgat
    14101 aatcgcaaac aacagactgc ccatggtgac gtttttcacc accgcagcac cagcttcagt
    14161 agcgttgtaa tgccagagcg taatgctgtc gagatagctg aacacggtga tcaaatcaga
    14221 ccaaattgcc cagaacatga caccgaacag cgcaaacatc agcaacatgg taatacgcag
    14281 cgtctgctgg ttaacctgct ccagcgcaat ggtgggttct tccggcggtt cggcaccttc
    14341 tgcgccctct ttcaccagat tctgccgacg cgccagtgca cgacgccagg cgatacgccg
    14401 cgccgctacg cttaagccgc gcaataccgt ctggtacagc aggttccaga tgatcaccaa
    14461 ataaacggtt tcaatccaac gcccggagag gcgcagcgtg gtgtagaagt aacccgtggc
    14521 ggtcagcacc atcaacgcaa tcgggataat cgacagcacg gtaatggtga ccagtcgcat
    14581 ggtgtgcgac tctttatcac gccagctttc gcggcacatc ggccacacca ggaaggcaat
    14641 cagcagcagg ttgaagaaaa tcatcgcctg ccccagcaca tcatccatca gatgcagcgg
    14701 ggagagttct gccaccacag accagaaatg gatcggcagc aacgcgaggc tgatgcggac
    14761 aatttgccga cgccagtggc tggtctgctg ttccggcata ccaaagtgac gcacggcaac
    14821 accgtttttc tccagcactt tccagcacag gccaaacacc agccagaaaa tcgccagttt
    14881 tttgctgaac gaccacaata actcgctgat attgagctgc atagtcagca gaatcaggcc
    14941 gacagcgaga ataatcaggc acaccggcag tgcgcggatt aggtcaataa gaatcgcttt
    15001 tggcgtattg agctggctat cgttacgcag ggaacctacc gccgaagcca gtttttgttg
    15061 atatgctttc agccagccta aacgccagtg aatcagcccg gcaatcaaca gcagcggcaa
    15121 accagcgagg aaggcgatga atacggcggg ccaggctttt tcccagttca ccgtgatttt
    15181 catcgactta aattcatctt tcagcgtttg cgggaacgct ttaatccagt cccagtccat
    15241 cggacggtta ctgttcaccc aaaaaatttg ctgcgtcagg atggatttca ggtttttcga
    15301 tacgctcatt aactgctgct ggttaatttg caggttaatg gccatcatca gctgattacc
    15361 caactgtttg ttaagctgat ccagcaattc acggcgcata tcaactactt gcaataacgc
    15421 atcgtgaact tcgctgttaa cttcattggt gtgaccttct tccagtttgc tgacgaacgc
    15481 atcgctctgg aacagcgcgt cacgctgctg gttgacttca aactgttcga gacgcaaatc
    15541 cgcgatgcgg ttggtcatgt tttccagttc atccgccgag ggcagcgttt gttgttgctg
    15601 gtaaagaata cgagacaaca gcaggctgcc cttcaggacg gcaatctgct ctttaatatt
    15661 gcgttccgat tgcagcgcac gctccagcca gtttttaact ttgatgtttt gctgcatcaa
    15721 ctgattaccg ttttcagtcg cggtaatcag acgctgactt aactgctggt taatttccag
    15781 ttcctgcttc accagcggat tagcctgaat acgcgcggct tcatccgggg agacagcttc
    15841 ctgcgccgtt ttctcggtta aagtcaggcg cttgctgttt accgcttctt gcaacagttg
    15901 taactggtgc tccagacggg cgctgttcgc cgtcacgtaa tcacgctgct tttgcaaggt
    15961 atcctgcaag acagtgttcc cttccaggct tttacgctgc tggtcaatct cggcattcag
    16021 caaagcctgc tggacctgca ttaacacttt ctggctggga cgtaaggcag tctcgccgac
    16081 atcagtccca tccagacgac tgcgaatttg ttgcagctgc tgcgaagcgt tatacatcgc
    16141 attttgcacg cgttcgggct gcgtctgtaa cgaaaccagt tggctgttat aagacgccag
    16201 atcattttgt gcgttttgca aatcgtccag cgcctgggca acgcgagttt ccagctggcg
    16261 caacgaaagc gtgctcagaa ttttgcgcgt ttcttcgtcg ttatcgacat cactaagtgc
    16321 tgttaacgcc gcggtcgcct ggcgcatttt ttccggcgct tcagcgactt tttgccgtag
    16381 ctgaactgtc tcttctttta cgcgatcgat tttatcgagg gtggctaatg tatctgtcag
    16441 atcctgctgc accagtttgt cctgagcaga aagatctttt tgtttattca gtgagtcaag
    16501 ttgtgcctgc aggtccgctt ttgtcggtaa atcaccattc gatgacgccc gtgcaaacgc
    16561 cgtgttctgg cataacaaga caaaaacaaa agcaataaat gctgaaaaaa caaaatgccg
    16621 tgatcgtttg taatactgga acatagtcat gatgaatgaa ggtttctgaa cctgaagaac
    16681 gacctgaaaa agtcaaaccg caagaatatc acgacgcagt gaaccagaat agcaacgacg
    16741 aaaatgtcca ggaaaattcc tggagtcaga ttcagggtta ttcgttagtg gcagggttac
    16801 gaagcgtggg gcacaggaga tacatctcca gtaagatggc gacgtaatcg cgggcttctt
    16861 ttttaagatc aaaagattgc ggggcaaaga gccagttttc catcaggccg gaaatatagc
    16921 cgcgcataat aattgctgcg cgacgcgtca ttaaatccgc aggcaacatt ttcgcttcaa
    16981 tacaatgttt taacgtttgt tctatacggt cataactttc cagacagaga ttacgttgtg
    17041 cctgttgcac aacagccatt tcgccgacaa attcgcattt gtggaatata atctccatca
    17101 ataatcgacg ccgttcttct gtcaccgtgg actcaagaac atgaattaat atctctctta
    17161 atactgagag tggatcgcca gggaattttg cctgatactc aagctcgagt tcaccaatat
    17221 tggattctga cagttcccag atctcactga acaaatccga cttgtcttta aaatgccagt
    17281 agattgcacc gcgcgtaacg ccagctgctt ttgcaatctc acccagcgag gtggatgata
    17341 ccccctgctg tgagaaaaga cgtagagcca catcgaggat gtgttggcgc gtttcttgcg
    17401 cttcttgttt ggtttttcgt gccatatgtt cgtgaattta caggcgttag atttacatac
    17461 atttgtgaat gtatgtacca tagcacgacg ataatataaa cgcagcaatg ggtttattaa
    17521 cttttgacca ttgaccaatt tgaaatcgga cactcgaggt ttacatatga acaaaaacag
    17581 agggtttacg cctctggcgg tcgttctgat gctctcaggc agcttagccc taacaggatg
    17641 tgacgacaaa caggcccaac aaggtggcca gcagatgccc gccgttggcg tagtaacagt
    17701 caaaactgaa cctctgcaga tcacaaccga gcttccgggt cgcaccagtg cctaccggat
    17761 cgcagaagtt cgtcctcaag ttagcgggat tatcctgaag cgtaatttca aagaaggtag
    17821 cgacatcgaa gcaggtgtct ctctctatca gattgatcct gcgacctatc aggcggcata
    17881 cgacagtgcg aaaggtgatc tggcgaaagc ccaggctgca gccaatatcg cgcaattgac
    17941 ggtgaatcgt tatcagaaat tgctcggtac tcagtacatc agtaagcaag agtacgatca
    18001 ggctctggct gatgcgcaac aggcgaatgc tgcggtaact gcggcgaaag ctgccgttga
    18061 aacttcgcga atcaatctgg cttacaccaa agtcacctct ccgattagtg gtcgcattgg
    18121 taagtcaaac gtgacggaag gcgcattggt acagaacggt caggcgactg cgctggcaac
    18181 cgtgcagcaa cttgatccga tctacgttga tgtgacccag tccagcaacg acttcctgcg
    18241 cctgaaacag gaactggcga atggcacgct gaaacaagag aacggcaaag ccaaagtgtc
    18301 gctgatcacc agtgacggca ttaagttccc gcaggacggt acgctggaat tctctgacgt
    18361 taccgttgat cagaccactg ggtctatcac cctacgcgct atcttcccga acccggatca
    18421 cactctgctg ccgggtatgt tcgtgcgtgc acgtctggaa gaagggctta atccaaacgc
    18481 tattttagtc ccgcaacagg gcgtaacccg tacgccgcgt ggcgatgcca ccgtactggt
    18541 ggttggcgcg gatgacaaag tggaaacccg tccgatcgtt gcaagccagg ctatcggcga
    18601 taagtggctg gtgacagaag gtctgaaagc aggcgatcgc gtagtaataa gtgggctgca
    18661 gaaagtgcgt cctggtgtcc aggtaaaagc acaagaagtt accgctgata ataaccagca
    18721 agccgcaagc ggtgctcagc ctgaacagtc caagtcttaa cttaaacagg agccgttaag
    18781 acatgcctaa tttctttatc gatcgcccga tttttgcgtg ggtgatcgcc attatcatca
    18841 tgttggcagg ggggctggcg atcctcaaac tgccggtggc gcaatatcct acgattgcac
    18901 cgccggcagt aacgatctcc gcctcctacc ctggcgctga tgcgaaaaca gtgcaggaca
    18961 cggtgacaca ggttatcgaa cagaatatga acggtatcga taacctgatg tacatgtcct
    19021 ctaacagtga ctccacgggt accgtgcaga tcaccctgac ctttgagtct ggtactgatg
    19081 cggatatcgc gcaggttcag gtgcagaaca aactgcagct ggcgatgccg ttgctgccgc
    19141 aagaagtaca gcagcaaggg gtgagcgttg agaaatcatc cagcagcttc ctgatggttg
    19201 tcggcgttat caacaccgat ggcaccatga cgcaggagga tatctccgac tacgtggcgg
    19261 cgaatatgaa agatgccatc agccgtacgt cgggcgtggg tgacgttcag ttgttcggtt
    19321 cacagtacgc gatgcgtatc tggatgaacc cgaatgaact gaacaaattc cagctaacgc
    19381 cggttgatgt cattaccgcc atcaaagcac agaacgccca ggttgcagct ggtcagctcg
    19441 gtggtacgcc gccggtgaaa ggccaacagc ttaacgcctc tattattgct cagacgcgtc
    19501 tgacctctac tgaagagttc ggcaaaatcc tgctgaaagt gaatcaggat ggttcccgcg
    19561 tgctactgcg tgatgtggcg aaaattgagc tgggtggtga gaactacgac atcatcgcag
    19621 agtttaacgg ccaaccggct tccggtctgg ggatcaagct ggcgaccggt gcgaacgcgc
    19681 tggataccgc tgcggcaatc cgtgctgaac tggcgaagat ggaaccgttc ttcccgtcgg
    19741 gtctgaaaat tgtttacccg tatgacacca caccgttcgt gaaaatctct attcacgaag
    19801 tggttaaaac gctggtcgaa gcgatcatcc tcgtgttcct ggtaatgtat ctgttcttgc
    19861 agaacttccg cgcgacgttg attccgacca ttgccgtacc ggtggtattg ctcgggacct
    19921 ttgccgtcct tgccgccttt ggcttctcga taaacacgct aacaatgttc gggatggtac
    19981 tcgccatcgg cctgttggtg gatgacgcta tcgttgtggt agaaaacgtt gagcgtgtga
    20041 tggcggaaga aggtttgccg ccaaaagaag ccacccgtaa gtcgatgggg cagattcagg
    20101 gcgctctggt cggtatcgcg atggtactgt cggcggtatt cgtaccgatg gccttctttg
    20161 gcggttctac tggtgctatc tatcgtcagt tctctattac cattgtttca gcaatggcgc
    20221 tgtcggtact ggtggcgttg atcctgactc cggctctttg tgccaccatg ctgaaaccga
    20281 ttgccaaagg cgatcacggg gaaggtaaaa aaggcttctt cggctggttt aaccgcatgt
    20341 tcgagaagag cacgcaccac tacaccgaca gcgtaggcgg tattctgcgc agtacggggc
    20401 gctacctggt gctgtatctg atcatcgtgg tcggcatggc ctatctgttc gtgcgtctgc
    20461 caagctcctt cttgccagat gaggaccagg gcgtatttat gaccatggtt cagctgccag
    20521 caggtgcaac gcaggaacgt acgcagaaag tgctcaatga ggtaacgaat tactatctga
    20581 ccaaagaaaa gaacaacgtt gagtcggtgt tcgccgttaa cggcttcggc tttgcgggac
    20641 gtggtcagaa taccggtatt gcgttcgttt ccttgaagga ctgggccgat cgtccgggcg
    20701 aagaaaacaa agttgaagcg attaccatgc gtgcaacacg tgctttctcg caaatcaaag
    20761 atgcgatggt tttcgccttt aacctgcccg caatcgtgga actgggtacc gcaaccggct
    20821 ttgactttga gctgattgac caggctggcc ttggtcacga aaaactgact caggcgcgta
    20881 accagttgct tgcagaagca gcgaagcacc ctgatatgtt gaccagcgta cgtccaaacg
    20941 gtctggaaga taccccgcag tttaagattg atatcgacca ggaaaaagcg caggcgctgg
    21001 gtgtttctat caacgacatt aacaccactc tgggcgctgc atggggcggt agctatgtga
    21061 acgactttat cgaccgcggt cgtgtgaaga aagtttacgt catgtcagaa gcgaaatacc
    21121 gtatgctgcc ggatgatatc ggcgactggt atgttcgtgc tgctgatggt cagatggtgc
    21181 cgttctcggc gttctcctct tctcgttggg agtacggttc gccgcgtctg gaacgttaca
    21241 acggcctgcc atctatggaa atcttaggcc aggcggcacc gggtaaaagt accggtgaag
    21301 caatggagct gatggaacaa ctggcgagca aactgcctac cggtgttggc tatgactgga
    21361 cgggaatgtc ctatcaggaa cgtctctccg gcaaccaggc accttcactg tacgcgattt
    21421 cgttgattgt cgtgttcctg tgtctggctg cgctgtacga gagctggtcg attccgttct
    21481 ccgttatgct ggtcgttccg ctgggggtta tcggtgcgtt gctggctgcc accttccgtg
    21541 gcctgaccaa tgacgtttac ttccaggtag gcctgctcac aaccattggt ttgtcggcga
    21601 agaacgcgat acttatcgtc gaattcgcca aagacttgat ggataaagaa ggtaaaggtc
    21661 tgattgaagc gacgcttgat gcggtgcgga tgcgtttacg tccaatcctg atgacctcgt
    21721 tggcgtttat cctcggcgtt atgccgctgg ttatcagtac tggtgctggt tccggcgcgc
    21781 agaacgcagt aggtaccggt gtaatgggcg ggatggtgac cgcaacggta ctggcaatct
    21841 tcttcgttcc ggtattcttt gtggtggttc gccgccgctt tagccgcaag aatgaagata
    21901 tcgagcacag ccatactgtc gatcatcatt gatacaacgt gtaatcacta aggccgcgca
    21961 agcggccttt tttatgcata acctacgaac attaaggagt attgaaccac caact
//
LOCUS       DADTFG010000082        21745 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.082, whole genome shotgun sequence.
ACCESSION   DADTFG010000082 DADTFG010000000
VERSION     DADTFG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.082"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            451..1518
                     /locus_tag="J1138_002826"
     CDS             451..1518
                     /locus_tag="J1138_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA8454596.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVMAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1515..4250
                     /gene="rbbA"
                     /locus_tag="J1138_002827"
     CDS             1515..4250
                     /gene="rbbA"
                     /locus_tag="J1138_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBA8454597.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4250..5374
                     /locus_tag="J1138_002828"
     CDS             4250..5374
                     /locus_tag="J1138_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBA8454598.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLLPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(5484..6335)
                     /locus_tag="J1138_002829"
     CDS             complement(5484..6335)
                     /locus_tag="J1138_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HBA8454599.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLGK"
     gene            complement(6367..6636)
                     /locus_tag="J1138_002830"
     CDS             complement(6367..6636)
                     /locus_tag="J1138_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBA8454600.1"
                     /translation="MLSKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCEGDDENEAIQTLTTLFRDRFGEAE"
     gene            complement(6626..8134)
                     /locus_tag="J1138_002831"
     CDS             complement(6626..8134)
                     /locus_tag="J1138_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBA8454601.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GNVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDKHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLLQPGEHAGWV
                     SESLCKTLGFSQSVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAVSLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(8127..9485)
                     /locus_tag="J1138_002832"
     CDS             complement(8127..9485)
                     /locus_tag="J1138_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA8454602.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(9562..9843)
                     /locus_tag="J1138_002833"
     CDS             complement(9562..9843)
                     /locus_tag="J1138_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA8454603.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(9840..10313)
                     /locus_tag="J1138_002834"
     CDS             complement(9840..10313)
                     /locus_tag="J1138_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA8454604.1"
                     /translation="MQGIQFQENFIQRLPAGLSVEQIIHQLAQPLVTAELVVPDFADH
                     VLEREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVVFADMGGDSDPVPVR
                     VIFLLALGESNKQLNALGWIMEMIQDTPFMRALLTMETTEIHTAILNKMKERGEI"
     gene            complement(10338..11084)
                     /locus_tag="J1138_002835"
     CDS             complement(10338..11084)
                     /locus_tag="J1138_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA8454605.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(11283..11684)
                     /gene="nikR"
                     /locus_tag="J1138_002836"
     CDS             complement(11283..11684)
                     /gene="nikR"
                     /locus_tag="J1138_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBA8454606.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(11690..12496)
                     /gene="nikE"
                     /locus_tag="J1138_002837"
     CDS             complement(11690..12496)
                     /gene="nikE"
                     /locus_tag="J1138_002837"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBA8454607.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(12493..13257)
                     /gene="nikD"
                     /locus_tag="J1138_002838"
     CDS             complement(12493..13257)
                     /gene="nikD"
                     /locus_tag="J1138_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBA8454608.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(13257..14090)
                     /gene="nikC"
                     /locus_tag="J1138_002839"
     CDS             complement(13257..14090)
                     /gene="nikC"
                     /locus_tag="J1138_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBA8454609.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(14087..15031)
                     /gene="nikB"
                     /locus_tag="J1138_002840"
     CDS             complement(14087..15031)
                     /gene="nikB"
                     /locus_tag="J1138_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBA8454610.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRTSMLDVAGQRHVTWARLRGLSD
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(15031..16605)
                     /gene="nikA"
                     /locus_tag="J1138_002841"
     CDS             complement(15031..16605)
                     /gene="nikA"
                     /locus_tag="J1138_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBA8454611.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVEYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(16716..17303)
                     /gene="acpT"
                     /locus_tag="J1138_002842"
     CDS             complement(16716..17303)
                     /gene="acpT"
                     /locus_tag="J1138_002842"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBA8454612.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(17358..18407)
                     /locus_tag="J1138_002843"
     CDS             complement(17358..18407)
                     /locus_tag="J1138_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA8454613.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPRFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            18506..19756
                     /locus_tag="J1138_002844"
     CDS             18506..19756
                     /locus_tag="J1138_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8454614.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(19760..20317)
                     /gene="dcrB"
                     /locus_tag="J1138_002845"
     CDS             complement(19760..20317)
                     /gene="dcrB"
                     /locus_tag="J1138_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBA8454615.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(20390..21055)
                     /locus_tag="J1138_002846"
     CDS             complement(20390..21055)
                     /locus_tag="J1138_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBA8454616.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            21276..21521
                     /gene="tusA"
                     /locus_tag="J1138_002847"
     CDS             21276..21521
                     /gene="tusA"
                     /locus_tag="J1138_002847"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBA8454617.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
BASE COUNT         5096 a         5678 c         5884 g         5087 t
ORIGIN      
        1 tttcagaata aattatttta tattcggtat ttattcgcac atcaggcgat aataacgcta
       61 tgtgatagtc atcaggtttc cctgttacgg aaacctgtgc aacagaatct gtgaacctgt
      121 acagaatatt tttggaaaaa acaccagcga acggaagaac atcgcgatgt atcacaagtt
      181 gatggcaatc aaatagacaa catgaatata actgttagta tttaattgat ttcacttatg
      241 agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac tacccgtttt
      301 cactaagcct tccccattgg cgctcaatgc tctgtgcgct ggtcgtattt gccatttcct
      361 tcttatcccg gatggaagaa tccgctgaac atcgttcggt ggcagttgta acacgcgtcg
      421 attgcttaca gggattatgg cgggtcgatt atggataaga gtaagcgcca tctggcgtgg
      481 tgggttgtcg gggcactggc ggtggctgcc gtcatggcgt ggtggctgtt gcgcccggcg
      541 ggtgtgccgg aaggctttgc cgtcagcaat gggcgcattg aagcgacgga agtggatatc
      601 gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca gtttgttcga
      661 gaaggtgaag tgctggcgaa gatggatact cgcgtgttgc aggaacagcg actggaagcc
      721 atcgcgcaaa tcaaagaagc acaaagtgcc gttgccgccg cgcaggcttt gctggagcaa
      781 cgccaaagcg aaactcgtgc cgcacagtcg ctggttaatc aacgccaggc agaactggac
      841 tccgtagcaa aacgtcatac gcgttcccgc tcactggcac aacggggggc aatttctgcg
      901 caacagctgg atgacgaccg cgccgccgct gagagcgccc gagctgcgct ggaatcggca
      961 aaagctcagg tttctgcttc taaagccgct atagaggcgg cacgcaccaa tatcattcag
     1021 gcgcaaactc gcgtcgaagc ggcacaagcc actgaacggc gcattgccgc agatatcgat
     1081 gacagcgaac tgaaagcccc gcgtgacgga cgcgtgcagt atagggttgc cgagccaggc
     1141 gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg atctcagcga cgtctatatg
     1201 accttctttc tgccaaccga acaggcgggc acgctgaaac tgggcggtga agcacgtttg
     1261 attcttgatg ctgcaccaga tctgcgtatt cctgcaacca tcagttttgt cgccagcgtc
     1321 gcccagttca cgccaaaaac cgtcgaaacc agcgatgaac ggctgaaact gatgttccgt
     1381 gtcaaagcgc gtatcccacc ggaattactc cagcagcatc tggaatatgt caaaaccggt
     1441 ttgccgggcg tagcgtgggt gcgggtgaat gaagaacttc cgtggcctga cgacctggtg
     1501 gtgaggttgc cgcaatgacg catctggaac tggttcccgt cccgcctgtc gcgcaactgg
     1561 cgggcgtgag ccagcattat ggaaaaaccg ttgcgctgaa caatatcact ctcgatattc
     1621 cggcccgctg catggttggg ctgattggtc cggatggcgt cgggaagtcg agtttattgt
     1681 cattgatttc cggtgctcgt gtcattgagc agggcaatgt gatggtgctg ggcggcgata
     1741 tgcgcgatcc gaagcatcgc cgtgacgtct gcccgcgcat cgcctggatg ccgcaggggc
     1801 tgggtaaaaa cctctaccac accttgtcgg tgtatgaaaa cgtcgatttt ttcgcccgcc
     1861 tgttcggtca cgacaaagcg gagcgggaag tgcgaattaa tgagctgctg accagcaccg
     1921 gattagcacc gtttcgcgat cgtccggcag ggaaactctc cggcgggatg aagcaaaaac
     1981 ttgggctgtg ctgtgcgtta atccacgacc cggaattgtt gattcttgat gagccaacaa
     2041 cgggggttga cccgctctcc cgcgcccagt tctgggatct gatcgacagc attcgccagc
     2101 ggcagagcaa tatgagcgtg ctggttgcca ccgcctatat ggaagaggcg gaacgcttcg
     2161 actggctggt agcgatgaat gccggagaag tgctggcaac tggcagcgcc gaagagttgc
     2221 ggcagcaaac gcaaagcgcc acgctggaag aagcatttat aaatctgtta ccgcaagcac
     2281 aacggcaggc gcatcaggcg gtagtgatcc caccgtatca acctgaaaac gcagagattg
     2341 ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt cgttgccgtt gatcacgtca
     2401 atttccgcat tccacgcggg gagatttttg gttttcttgg ttcgaacggc tgtggtaaat
     2461 ccaccaccat gaaaatgctt accggattgc tgcccgccag cgaaggtgag gcgtggctgt
     2521 tcgggcaacc ggttgatcca aaagatatcg atactcgtcg ccgggtgggc tatatgtcgc
     2581 aggcgttttc gctctataac gaactcaccg tgcggcaaaa cctggagtta catgctcgtc
     2641 tgtttcatat cccggaagcg gaaattcccg cgcgagtggc tgaaatgagc gagcgtttta
     2701 agctcaacga cgtcgaagat gtattgccgg agtcattgcc gctcggcatt cgccagcggc
     2761 tatcgctggc ggtggcggtg attcatcgcc cggagatgtt aatccttgat gaaccgactt
     2821 ctggcgtcga tccggtggcg agggatatgt tctggcagtt gatggtggat ctctcgcgcc
     2881 aggacaaagt gactatcttc atctccaccc actttatgaa cgaagcggaa cgttgtgacc
     2941 gtatctcact gatgcacgcc ggaaaagtgc tcgccagcgg tacaccgccg gaactggttg
     3001 agaaacgcgg agccgccagt ctggaagagg catttatcgc ttatttgcag gaagcggcag
     3061 ggcagagcaa cgaagctgaa gcgccgccgg tggtacacga caccacccac gcgccacgtc
     3121 agggattcag cctgcgccgt ctgtttagct acagccgccg cgaagcgctg gaactgcgac
     3181 gcgatccggt acgttcgacg ctggcgctga tgggaacggt gatcctgatg ctgataatgg
     3241 gttacggcat cagtatggat gtggaaaacc tgcgctttgc ggtgcttgac cgcgatcaaa
     3301 ccgtcagtag ccaggcgtgg acgctcaatc tctccggttc ccgttacttt atcgaacagc
     3361 caccgctcac cagttatgac gagcttgatc gtcggatgcg tgcgggcgat atcacggtgg
     3421 caattgagat ccctcccaat ttcgggcgcg atatcgcgcg tggtacgccc gtggaactcg
     3481 gtgtctggat cgacggtgcg atgccgagcc gtgccgaaac ggtaaaaggt tacgtgcagg
     3541 ccatgcacca gagctggcta caggatgtgg cgagccgaca atccacgccc gccagccaaa
     3601 gcgggctgat gaacattgag acgcgttatc gctataaccc ggacgtaaaa agcctgccgg
     3661 cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat cccgtcaatg ctaagcgccc
     3721 ttagcgtggt gcgggaaaaa gagctagggt cgattatcaa cctttacgtg actcccacca
     3781 cccgtagcga atttttactt ggcaaacagc tgccatacat tgcgctgggg atgctgaact
     3841 ttttcctgct ctgcgccctg tcggtgtttg tgtttggcgt accgcataaa ggcagtttcc
     3901 tgactctcac cctggcggcg ctgctgtata tcatcatcgc caccggaatg gggctgctga
     3961 tctccacctt tatgaaaagc cagattgccg ccattttcgg tacggcgatt atcacgttga
     4021 tcccggcgac acagttttcc gggatgatcg atccggtagc ttcgctggaa ggtccaggac
     4081 gttggatcgg cgaggtttac ccgaccagtc attttctgac tatcgcccgc ggaacgttct
     4141 cgaaagcgct ggatctgact gatttgtggc aactttttat cccgttgctg atagccatac
     4201 cgctggtgat gggcttaagc atcctgctgc tgaaaaaaca ggagggatga tgcgccattt
     4261 acgtaatatt tttaatctgg gtatcaaaga gttgcgcagt ctgctcggtg ataaagcgat
     4321 gctgacgctg attgtcttct cgtttacggt gtcggtgtat tcgtcagcga ccgttacgcc
     4381 aggatcgttg aacctcgcgc cgatcgccat tgccgatatg gatcaatcgc agttatcgaa
     4441 ccggatcgtt aacagcttct atcgcccgtg gtttttgcta ccggagatga tcaccgccga
     4501 tgagatggat gccgggctgg acgccggacg ttataccttc gcgataaata ttccgcctaa
     4561 ttttcagcgt gatgtcctcg ccggacgcca gccggatatt caggtgaacg ttgatgccac
     4621 gcgcatgagc caggcattta ccggcaatgg gtatatccag aatattatca acggtgaagt
     4681 gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg ttggtatcgc tggaaacacg
     4741 gatgcgcttt aacccgaacc ttgatcccgc gtggtttggc ggggtgatgg cgatcatcaa
     4801 caacattacc atgctggcga ttgtattgac cggatcggcg ctgatccgcg agcgtgaaca
     4861 cggcacggtg gaacacttac tggtgatgcc gataacgccg tttgagatca tgatggcaaa
     4921 ggtctggtcg atggggctgg tggtgctggt ggtgtcggga ttatcgctgg tgctgatggt
     4981 gaaaggcgtg ctgggcgtac cgattgaagg ctcgatcccg ctgtttatgc tgggcgtggc
     5041 gctcagtctg tttgccacca cgtcaatcgg catttttatg gggacgatag cgcgttcaat
     5101 gccgcaactg gggctgctgg tgattctggt gctgctgccg ctgcaaatgc tttccggtgg
     5161 atccacgcca cgcgaaagta tgccgcagat ggtgcaggac attatgctga ccatgccgac
     5221 gacacacttt gttagcctcg cgcaggccat tctctaccgt ggtgccggat tcgaaatcgt
     5281 ctggccgcag ttcctgacac tgatggcaat tggcggcgcg tttttcacca tcgcactgct
     5341 gagattcagg aagacaattg ggacaatggc gtaattacag ttgcccgatg cgtaagctta
     5401 tagggtctac gcattggtgc aatttattga attttagtgg ttttgtaggc cggataaggc
     5461 gtcacgccgc atccggcgaa gatttactta cccaaccgat gcccctcatt catcatcagg
     5521 attttgctat acacatcttc ctcgacggcg ttttttgctg atgccatcac ccgtgccaga
     5581 ttcgcctcgt tgtgattatt cacatacgct ttgccgactg cggtgataaa cgctttgcgc
     5641 agatcgctgg cgatattcac tttagcgacg cgataattaa cgaaactgcg taatatctcc
     5701 ggggcgatac cggagccgcc gtgaataacc agcggcaccg ggctgacttc ggcaatgcgc
     5761 ttcagcaggg ggatatcgat ccgcggtata tcgtccaggc catgaacgtt accaatagaa
     5821 actgccagca tgtcgcaacc ggtgcgttcg acaaaggtct tcaccttttc cggttccgtt
     5881 ttgcaatcgg cttcgctgac gtgatcgtct tctttgccga gaatcgcccc cagctccgct
     5941 tcaaccggta cgccatagga tttacagaaa tcgacagctt cctgggtgaa agcgatattc
     6001 tcttcaaaag agaatgcggc accgtcaatc atgaccgagg taaagcccgc gcgcaccgcc
     6061 tgtttaacat cctcctgagt tttcccgtga tcaagatgaa gactgaccgg aacatccatt
     6121 ttatcagcgt gacgtttcac gatttcataa atgtaatcgt atcccgacag ttgtgcattg
     6181 gtgggcgcaa tctgaatgaa gttgggtaat cctgaacgct caatggcgtt taaaattgaa
     6241 atcgttgtct ccagattggt ggtattaaat gcgcccgcta atacccgttt ttcctgaatg
     6301 cggtcgagca gtatccgtcc attaactaat ggcatattga aaacctcatc ttttaatatc
     6361 agttaattat tcagcttcac cgaagcggtc gcgaaataag gtagtgagag tttgtattgc
     6421 ctcgttttca tcatcacctt cgcagcgtat ttccaccgtt tttccgcctt ttattccggc
     6481 gcgcataata ttcatcatgc tggtggcatt aatatcatta ccttcatagt gcagcgtcac
     6541 tttacagcta tattttttgg cggctttcgc cagacaggcc gccggacgtg catgcaggcc
     6601 agtactgttt ctgacttcca cggttttact caacatagtg ctcctcccga tgatgtaatg
     6661 gcatcacgcc ttcttgctgt aatttcaact gataccagct tttccatttg tgctgataac
     6721 gctgtaattg atggtatctc gcccgattcg gtaacaaacg ttgggctatg gtgacttgcg
     6781 gcggtggaat atccggtgcg gcggttaata atgctccgac agaaaccgcc tcgttaaatg
     6841 caattgcttc aaccggacgc tgtaaaatat ccgcctttaa ttgcagccaa aggggattat
     6901 gggttgccgg acctatcact tttaacgcgc tggtgccggg cacattaaaa caggcgaagc
     6961 agttggcaaa ttccatggtt aatcccagtg agacgctggc aattaacata tcgatattta
     7021 acgtatcgcc aagcccataa aataatcctc gggtatggcg atttttatag ggcgaacccg
     7081 aaccgcgaag atgcggaata aaaacgggaa tgtcatcgag cgaccagttc cccgccaggt
     7141 aatccgcata cccacgattg agcgacacgg cgatctcctc atcggttagc cggaacgtgt
     7201 tacgcagcca ctccagcgcg aaaccgccca cgggcagaga agcaaacagg gtgaactggt
     7261 tggcaagcga gtaacagccg tttgccagct tgtcacgttt ggcctgttca tccagcgtcg
     7321 gttgtgtatc cagttgcaga atgccttccg tggtgcccgt cgagttaagg atatcgcccg
     7381 gcatcatctg caacgcccgc gcacccacca tatggtcatg tccggccagc gtcacgctga
     7441 ccgattgcga gaacccaagc gttttgcaga gcgactcact gacccatccg gcgtgctcac
     7501 ctggctgcaa caatggcgca aatgctgaac agggaacgtg taacaacgcc gccgctttcg
     7561 ctgaccattc gcctttgcgc acgtccagac acagtgtacg gctggctaag gtgatatccg
     7621 ttcgctgttc gccagtcagc cgccagagca gcacttccgg tgcatgtagc cagcgcaggc
     7681 cgcgacgatt gcgcagcgga taatgctcca gcaaccaacg cattttgaag gcagagtaat
     7741 tgctgtgcag tggtaacccg caaatgtcat aaagtgccgc actgtctgcc tcgctaagcg
     7801 ttgccagata ctcttcaccg cgacggtcat accatgccag cattggcgtc agaatctcgc
     7861 catgtttgtc aagaaacacc cctgactcgc caaaactggc aatgctgatc cgcctgactg
     7921 gcagcggcga agcggcgttc agttgcgcta tcgcctggcg gacctcctgc cagagggcgt
     7981 cgatatcgaa atcgacattg ccctgtgggg agatctgttt tgccgtcacg aatttatgcg
     8041 cgcccaacgt cgttgcgtcc aggcaggaaa agcaggtgac tttgcaattg gtggtgccaa
     8101 tatcgataac gatagctgcg ctgttatcag gcataaaccg tgctccgcgt cattcggaaa
     8161 ataccataca gtaccagggc gataaccggc accagaccaa tggcgatgag attgccgttg
     8221 aagatttcca gcagccagaa gcggaacggg ttaccgccat caaggaaact ggagacctgg
     8281 ccgctggagc cttcggcaaa tttgaaatta acgtctttcg ccattccggt aataaacggc
     8341 gcgattttag tcgcaatcca cagatcggca ataatcaccg gaatcgccgc cagaacggtg
     8401 cggaaaatgt tgccacggct ggctaaagca atcatcgacg ccattaccgc caggttggca
     8461 aggtccccaa gcggcaggac ttcattaccc ggaaggacaa acgcgataaa gaccgagatt
     8521 ggcgtcagca gcagggcggt ggagataatc gccgggttac ctacggcaac ggcgatatcc
     8581 aggccgatat agagatcgtc acggtcaggg tatttggcat tgagatactt tttaatggct
     8641 tcagacagcg gtaataaacc ttccatcaga atgcgaacca tgcgtggcag gatgaacatc
     8701 acaccgccaa tgctgatgcc aagcagcaag acttttttga aatcgtatcc ggcaattacg
     8761 ccgagcagaa tgcccagaat agtaccaacc atcatcggtt cgccgaatat gccaaacttt
     8821 ttctgtacgg tttccggatc gatatgaata cgattgaggc cggggatatg gtcgatgatt
     8881 ttgtcgacca acagaccgac cggaaagaac accaccgaag agagcgtcgg caaagagatc
     8941 ccttccaggc caaagtattt ttgtaccagt ggcgcagacc agtcggcgag tttaagtact
     9001 accaccgcag caatcgccgc tgccagcaaa ccaaagaaca ggctgccggt gctgtaataa
     9061 accagcgtac cggcaagggc aaaatgccag tagttccaca tatctacgtc aacggtgcgg
     9121 gttttattta acgccagcat cgccacgtta atcagaatag tcaggggaat aacaaacggg
     9181 gcaattggcg aaccccatgt aatagcggaa agcggcggcc agcctaaatc caccaccggt
     9241 aaattaattc cggtgcgttc aaccatcgcc tgggccgctg gcccgacatt gctggtaaga
     9301 atatcgaaaa tggcataaat accgacaaaa ccaatgccga ccgttaatga cgaacgtaag
     9361 gctttagcgg gttttacttt aaaaaagaga gcaagaataa ataacaccaa cggcaacatt
     9421 accgtgggac caaatcccaa tatatattgc acaatattat agagggtatg cgcgatatcg
     9481 ttcataataa acctcttttt caggcgagag cgcacctcgc gttagcgcct gatggagtgg
     9541 gttgaaatta attattatta attacttttt aaggatcgaa acaatttgcg ccagtacttt
     9601 ctcttcgcca ataccggtaa taatcggcaa gccgttaacc accgggacgt cgagttcata
     9661 aggcacttta gtcgttgaga ctaccagtgc tacgccgtca gtatttccag gtaaagaggc
     9721 aacgttggtt tgggaataat tgacgttaag accgtgctct ttacaatatt ccatgacttt
     9781 ttctgttact gccgtggatg tggcaatacc cgtggcgcag acaaataaaa tggtttgact
     9841 catatttcac ctcgttcttt cattttgttt aagattgctg tgtgtatttc tgttgtttcc
     9901 atcgtaagca gggcgcgcat aaagggcgta tcctgaatca tctccataat ccagcccagg
     9961 gcatttaatt gtttattact ttcgcctaag gcgagcaaaa aaatcaccct gacaggtacg
    10021 ggatcagagt cgccgcccat atcggcaaac actaccggtt ccggcaggat accgacggca
    10081 attgcattgt gcctgacatg tttatggtcc gtgtgcggaa tggcgacaca cggtggttcg
    10141 gtaggcagtc ccgttgggta cgtcgcctca cgttccagta catgatcggc aaaatcaggg
    10201 accaccagtt cggcggtaac caacggttgc gctaactgat ggatgatctg ttcaacgctt
    10261 aaccctgccg gaagtcgttg aataaaattt tcctgaaact gtatgccttg cattttattc
    10321 ctcttaaaaa agtgtactta actcacttcc cgccgttgca gcacggtgcc aagatagtat
    10381 ttatttcctt ttaaccagac gttgccaaac tcaacgggta atcctcggga gaaaaagact
    10441 aactgctcca gatgcaaaac gggtgcatct tcactgatat ctaaaaaatg accgcgttca
    10501 ttaccaatta atcgcgcggc atagcgactt tccgagtaac gaatttttct ttgcgacaaa
    10561 ctttctattg ttggaaataa attgtgttga ttaaaatcga tttccacgat gccggggcat
    10621 agctcaatat tgatacggtt ctctatcagc atcgcttttt catcaccaat tgaacgtaaa
    10681 cgctcaaggt aaagaatatc ctgaccgggc gttattctta atttctctgc cacataacga
    10741 ttagccggtt caatccgtga cgtaataact tcagtggtaa aatgtatttt ctggctttcc
    10801 agcgattccg cgaatgacaa taatccttca cctaacggat atgccacgtt ctcttttttg
    10861 acaaatgttc ccttcccctg gacctgtatc aacacgcctt cttttaccag ttgggcgacc
    10921 gcttttttaa cggttccccg gctcacttcc agcatatcca taatctggta ttccgaagga
    10981 atgcgatcgt cttccaccag atccccggta taaatacttt ctcttatcca ctccaccact
    11041 tgttgataga gcggtatttg tgacgactta ctcaatgatt tcatggcgcg cacctgaaca
    11101 tttacagttg tctatggaca atcatggaca acttcaagcg tatagtaggt gaacaggcga
    11161 aaaagaggat cgaagcgtct cacattttag gggaccaaat cgtgatcaaa aaaatgtgac
    11221 gtgagtcgat atttggcaga agtctactgc gccgtcagga aataacggcg cgttgggagg
    11281 tgttaatctt ccttcggcag gcactgcaaa tgcccgtgcc gcacgccgcg ctgggcgatg
    11341 acgtcatcgg caaaatgctg cacgtcgccc atgtcacctt tcaacacggc gatttccaga
    11401 cagtcgtcgt gattaatgtg cacatgcaga gtagcaaccg agagatcgtg gtgatgatgc
    11461 tgggtggaga caatacggct ggctaagtcg cgtttttcgt gttcatacac atacgacagc
    11521 accgcgaaac cttgtgtgcc gtgttgttga gtggcttcct gcgccagagc gctacgcaga
    11581 atatcgcgga tcgcctcgga acggttgtta taaccacgac gctggctcag actgtccagc
    11641 gtctccagta aatcgtcatc aagcgtgatg gtgactcgtt gcatttgcgt taaacctttt
    11701 ctgtggtgcg acggcgcacg gggaatgcgg gtaataccgc gttttgtagc acacgtccgg
    11761 cgtcagagga aaaggttaat ttatctccca ccacctgggt ttcgacgatt tgtccgttgt
    11821 ccatcaccat cactcgctgg caaaagcgtt ccaccaggcg taagtcatga gtgatgaaca
    11881 ggcaggcggt gccaaactgt tgttgcagct ttttcagcag gcgaatgacg cccgcttgta
    11941 acacgagatc aaggttcgaa accgcttcat ccagaatcag cagtttcggt tcgaccgcca
    12001 gcgcgcgtgc caggcagaca cgctggagct ggccgccgct taactgcggt gggcgtttgt
    12061 cgagcacgct gtcgtcgaga tcgaccgcct tcagcatttc actggcgcgc gccagttgtt
    12121 cggctttttt cagcgatagc aggtggcgca tcggttcacg caggatctcg cgcacggttt
    12181 tgcgcggatt cacggcgctg atggagtcct gaaacaccat ttgaatatca cggcggaacg
    12241 ctttacgttg cgcgcgattg agcttcgcca acggttcgcc gcgccagcta atatttccct
    12301 gtgagggcga ctctaaccct accagcagcc gcgcgagggt actttttccg cagccgctgc
    12361 gccccagcag ggcgacggtt tcaccgcttt tcagggccag ggaaacgtta ttcagcaccg
    12421 cctggtgttg atgtttgccg ctaaatccac cgtgcgcgta gtgatgggaa aggtcggaga
    12481 cgttaagtaa agtcatgatg ccagctccat accgtagagg gcgagatgag cggaaaccag
    12541 gctgcgcgtt accgcatgtt tgggggcgtt aaacagcgtt tctacatcgc cctgttcaac
    12601 aatcttaccg tgagacatta ccgccacatc gtctgccaga cgcgctacca cgcccatatc
    12661 atgggtaacc agcagcattc ccggtgcttg tttttgcata atgctttcca gcagatcgag
    12721 gatgcgcgcc tgtgctacca cgtcgagatc ggtggtcggt tcatcggcga tgataaacgg
    12781 tgattcgcac agcaccgcca tcgcaatcat catgcgctgc aacatgccgc cgctcatctc
    12841 gaacgggtag agcttcagca cgcgcgcggc gttttccagc cccaccgctt ctatggcagc
    12901 ggtaagcgta gcgtcatcgg cgggtttccc cagcgccagg caggtttcac gcgcgtgggt
    12961 gtgcatggtg tgcagcggat taaaggcact gcgcgggttc tgcatgatgg tggcaatttt
    13021 gatgccgcgc agggcgcagg gagaaaccgg ttttccatcg gccaaaattt cccccgccgt
    13081 ctggcgaacg ccagcaggca gaatgcccag cgtcgcggcg caggtcagcg acttcccgct
    13141 gccactaccg ccgactaacg ccagcacacg cccacgttgc agggttaacg atacgccgtg
    13201 caccagcggc tgcgcggcct gtagcgcgat attacggagt tcaatctgtt gtggcattag
    13261 tgtgcgtgct ccgtcaccag atgagggtcc agatgatcgc gcagtgcgtc acccaccagg
    13321 ttaaaggcca tcacgctgat aaacagcgcc agccccggcc agaacatttg cagcggctgg
    13381 gtccagatat actgacgtgc atcgttaatc atcacgcccc attcggcggt cggcgcggtc
    13441 acgccgaggc cgaggaagga catccccgcg acgtgtagca tcatatgacc gatatccagc
    13501 gtcgccagca ccagcagcga ggggatcacc gcacctgcca gatgatcaac aaacacccgc
    13561 acatggcccg cgccagaaag ccgtgatgcc agcacaaact cgcgctggcg cagtgaaatc
    13621 accagactgc gcaccatgcg cgcgtaccat gcccagtgcg aaagggcgat agcgataatt
    13681 acgttggtca acccggtgcc aagcacgccg accataaaga acgacagaat cgaggttggg
    13741 aaggtcataa acatatccgc gacgcgcatg gtggcctgat caacgcgccc gccaatcagc
    13801 ccggcgctgc cgccaataat cagccctaat gtcagcacca acagcaggca ggccattacc
    13861 gagccgagcg acacgcgggt cgctgccatc agccgcgaga aaatatcgcg ccctaaatga
    13921 tcggtgccca gccagtgctg cgcatccggc gagagcaggc gcgacggcaa atcaatagcc
    13981 tgagggtcat acggcagcca ccactggctg gtgagcgcaa tcagcgccag cagggcgata
    14041 atgatcagcg ccaggcgaac cgaccagcgg gaggagagga aaaagttcac gcgtgcgctc
    14101 cttcatgacg acgaatacgc gggtccagcg cggcgttgag caaatcgaca atcaaattac
    14161 agaccacaaa aaccaccacc atcatcagcg taaagcattg gatcaccgga tagtcacggt
    14221 taaaaatcgc cgacaccgca tagcgcccga cgcccggcca ggcaaagatg ttttcgataa
    14281 tcatcgtccc gccaatcagt tcgccaatgt gcatccccac ggcagtgatc atcggcagcg
    14341 aggcattgcg cagaatatgg cggcgttcgg tctgtttgtc gctcagacca cgcagtcgtg
    14401 cccaagtgac gtgacgctga ccggcgacgt ccagcatact ggtgcgcagc aaacgcgcgt
    14461 taatcgccag cgacataaag gcgatggata ccgcaggcaa aatgatgtgc tgccagccgc
    14521 cgtaacccat tgcgggtagc cattgcagat acaccgaaaa cgccatcacc agtaaaaacg
    14581 ccagccagaa gttaggcatc gacacgccga gaaacgcgat gaaacggacg gcgaaatccg
    14641 gcagacgatc gcgatggcgc gctgcccaga taccgagcgg tacggaggtg agcagaatta
    14701 ataccagcgc cgcacctgca agttccagcg tggcgggcag gaaattcagc atatcgtcca
    14761 gcaccgggcg ttggctggcg aatgagatac caaagtcaag atgcagcgcc ttccacaacc
    14821 aggtgccata ctggacgtac agcggctgat ccagccccag catagtgcgg gtagaggcca
    14881 gcatctccgg cgtcggcggc aggttagata aacgcaaata atcaagcgcc gggtcgccgg
    14941 tgccgaggcg cagcatcaaa aaaatgatca ccgaggcggc gagcaccatc gggatcagca
    15001 gcagaaagcg gcgtaataca taacgcaaca ttaaggtttc accggtttaa tctgttcgaa
    15061 cgggatttcg gtggcgatcg gcgcgtaggg gatgttaccc agttccggtt ttgaaaccac
    15121 catcattgag atgtaactga taggcagata aaccgcctcg tcatgcagac gggtcagaat
    15181 gtcgcgatac agcgcctgac gttgcgtttc gtcatgggtc gccagcactt cgccgatctc
    15241 tttatcaatc agcggcttgt cggctaaacc ttgctgtgcc tggaagtcag cgtgcgacgg
    15301 tacgcgcatt gaactcagga aggcgtgtgg atcgtatggc gcgccccagg tgcggtggaa
    15361 aatcatgcca aaacgaccgt cgcgctgacg agcatagata ctgctctctt cttcgccaat
    15421 cagcgagaca tctgcgccaa tctggcgcat atcagcctga atgatttccg ccatcgattt
    15481 gcttaacgca tcggtgccga tgaacgacag ttcaatgcgc agcggctgac catttttctc
    15541 gcggatgtct ttgcctgcag gcagcgtcca accggctttt tccagcaacg ctttcgcttt
    15601 ctgcggatcg tactggcgcg gtttcaggcc gaggttggcg tagggcacag aaggggcaaa
    15661 cagggtgtcg gcgacctgct gggtaccata caacgcgtta tcaatcagcg attttttgtt
    15721 caccgcgtaa ttaagagctt cacggactgc cagctcgttg gtgggggctt tggcggtatt
    15781 gagcgccagc atcacggttt cgatcggctg tgacagttga gtgtggtaag ccgggttctg
    15841 gctaaagcgg gcgaaggtat cgagcggtaa taacccttcg tttccgtaca gcaggtcgat
    15901 atcgccagtt tcaaacgcca ccgcgcgggt agtcgggtcc gggatcacgt taaaggtgat
    15961 ctttttaatc gccggctttt cgccccagta gttttcgtta cggacgaaga catcgtactg
    16021 attcagtttc gattcctgta aaacccacgg cccggtgcca atcggcgctt taattccgtt
    16081 catggtttcg tggtttttaa actgcgaggg tgcgataaaa cggaaaggac ggggcagggc
    16141 cagttcttgc aggaaaggat agtaggcgct tttcagggtg atttgcagct cgtttttgct
    16201 gagtgctttg acatcaacaa tctggtttgc cagctccagc caggcgtgac gttgacggtt
    16261 atcgagcact gcacggaagt tttctgccgc cgcctctgca tcaaacggtt cgccgttgga
    16321 gaatttcacg tcatcacgca gggtgaaggt ccaggtttta ccatcttctg aatgagtcca
    16381 gctttttgcc agccacggga tcaccgaacc gtctgcctga tattccacca gcggttcata
    16441 aaccatgctc tgggcgaaca tttggttagg cgtgtaaagg tgcgggttta gtggcccgac
    16501 attcaccggc caggcggtgg tgatttcatc tggtgcagcg gcatggacga taaaagacgc
    16561 acaagccagc agcgcaaata gagtgcggcg gagtgtagag aacatggtaa ccccaatgga
    16621 ttaaaataga tggcggaaat aagtatgacg attttaagta ttcgtcatac tgattacctg
    16681 ttctggatca atagatgggc agtcgggtgg gcggatcagt taactgaatc gatccattgc
    16741 acactgtcgg cggtgagcgt aaagggggta ggggtgcaga tcgccaggct taaattttct
    16801 agctgacaat ggctgactga cagcgaggag ttataggtgc tgtctacgct gacgatttgc
    16861 caggcgctgc cgccacgctg tttaacgatg gcttctttgc gcgtccagat gcgccagaac
    16921 atttccagtt gctgatcagg atgcacggcg tccatctcgg catgttcccc aaggctgaat
    16981 acggcgttcg ccagccagcg ccagttggcg cgcgggcgaa tcacttcgat atcgcagccg
    17041 acttcgcctt catcgctcaa cagcagggcg atatcgtcac cactatggct taagttgaac
    17101 catagcggcg tttccggcgc gaatgcaggt ttgccttgtt cgccatagat gatctccggt
    17161 agcggggaaa gcgtgtgcga aagcaatgca cgccccgcca gccagcgttc acgtcgtgga
    17221 ccttgcggtg cttgctcgcg taaacccggt ggcagaggag ctgcgcttaa ggtcgaaact
    17281 ttccccagaa ctatccgata catatcaggg ccaacgttta atggaaaatg aaagtgcgta
    17341 tcgtaacact tgtcgcctca tcccggtaac cgacttttcg gtctgcctgg ccccagtaaa
    17401 atcgccagtt tgctgccgcc tttggtggtt tccatccaga ttttacacac gctggttaat
    17461 ggcaccgaaa gtagcatccc taccgggccg agcagccatc cccaaattaa caatgaaaga
    17521 aataccacca tggtggacat ccccaggcga tggcccatca tccgtggttc taaaatattg
    17581 ccgatgacca tatggaccac taaaaacaat gcgccgacca gaatacattc gtaaatacca
    17641 ttaaacagca gcacctgaat cattggcggt acggcggaaa ttaccgcacc gatattgggc
    17701 acgtagttga gcaaaaacgc cagtactgcc cacatcagcg caaactgcac ccccatcagc
    17761 tccagcccca gccagacgat tatgcctgtc cataaactga gtagcgtctt taacgccaga
    17821 taatgcgaaa cgcctttaag tgcgcggtgt agtccggcga tgtgaatctg tggattattc
    17881 aacgcgaaac gcattttgta agggacgtgg cgcacttcaa acagcataaa aactacggtc
    17941 atcaccagca aaagcacgct cgccattgcc ccggaaagcc cggtcattag cgccgtggtg
    18001 aaggtaacca ctttttccga gtccatccgc tgcagtattc gctccggcga catatgcaaa
    18061 ttaagaaaag gcaacatctc ctgcaattta aaaagtttgc gcgtcagctc cttattaaac
    18121 ctcggcagca tagagataaa ttcgttaaac gatgccgcca gtacgccgac cagcgcggtg
    18181 agtgcgatca gcatcaccac aaccacaatc gtaatggcaa cggggcgttg taccccccga
    18241 cgaataaacc aggtgacgag cgggttgaga acgatggcaa aaaacagcgc cagtaatagc
    18301 tgcacaatga tatctgccgc tgcgtgaatg cccgcgagga tcactaccag cgaggccaac
    18361 ttgagcagaa tgtgcatgcc cgttttatcg ggttgagggg tttccattgg ggcttccttg
    18421 tgactttttg tattaagtgt agcgggagtc acgccagcaa tattctgatt cttactgctg
    18481 attttcacgt cagcacatgg taaaaatgaa acactgttgt aaaaatgtgg tgatcctcat
    18541 gcccgaaccc gtagccgaac ccgcgctaaa cggattgcgc ctgaatttgc gcattgtctc
    18601 cattgtcatg tttaacttcg ccagctacct taccatcggg ttgccgctcg ctgtattacc
    18661 gggctatgtc catgatgtaa tgggatttag tgcattctgg gcagggttgg ttatcagcct
    18721 gcaatatttt gccaccttgc tgagccgtcc tcatgccgga cgttacgccg atttactggg
    18781 accaaaaaag attgtcgtct tcggtttatg cggctgcttt ttgagcggtc tgggatatct
    18841 gacggcagga ttaaccgcca gtctgcccgt catcagcctg ttattactgt gcctggggcg
    18901 cgtaatcctt gggattggac aaagttttgc cggaacggga tcgaccctgt ggggtgttgg
    18961 cgtggttggt tcgctacata tcgggcgggt gatttcgtgg aacggtattg tcacttacgg
    19021 ggcgatggcg atgggtgcgc cgttaggtgt cgtgttttat cactggggcg gattgcaggc
    19081 gttagcgtta atcattatgg gcgtggcact ggtggccatt ttgttggcga tcccgcgtcc
    19141 gacggtaaaa gctagtaaag gcaaaccgct gccgtttcgt gcggtgcttg ggcgcgtctg
    19201 gctgtacggt atggcgctgg cactggcttc cgccggattt ggcgttatcg ccacctttat
    19261 cacgctgttt tatgacgcta aaggttggga cggtgcggct ttcgcgctga cgctgtttag
    19321 ctgtgcgttt gtcggtacgc gtttgttatt ccctaacggc attaaccgta tcggcggctt
    19381 aaacgtggcg atgatttgct ttagcgttga gataatcggc ctgctactgg ttggcgtggc
    19441 aactatgccg tggatggcga aaatcggcgt cttactggcg ggggcggggt tttcgctggt
    19501 gttccctgca ttgggcgtag tggcggtaaa agcggttccg cagcaaaatc agggggcggc
    19561 gctggcaact tacaccgtat ttatggattt atcgcttggc gtgaccggac cactggctgg
    19621 gctggtgatg agttgggcgg gcgttccggt gatttatctg gcggcggcgg gactggtcgc
    19681 aatcgcgtta ttactgacgt ggcgattaaa aaaacggcct ccggtggaaa tacctgaggc
    19741 cgcctcatca tcttaaaact tactgaataa ccagcgtatt aatgatgttt tctgcggtgg
    19801 tctgtgcttt ttgctgattg tcagctggca gcgtaatttg catggttagc agttgattac
    19861 ccacgttacc cagaataacg gaagagtacg ccgtctggcc tttcgcggag ataatgctgt
    19921 ctaactgctg cattttgtga cctttcagct caatggcttt attggttacc acttgcagct
    19981 gcggatcgcg gctacgttgc tgatcttcca gacgcttcgc cagcaccgcc agatcttctt
    20041 tcggatcatc gcccatgatg acgatgactg ctttctgccc ggtggcgtcg gaccagacat
    20101 gcatgttatt ggcctgcgtt cccagcttac cgctctggtc ggtcatatcc gctggcagcg
    20161 agaaacttaa cttgccatca agcaggttga cgggattccc ggtcgcgtta ctttctgcga
    20221 ccgaaccctg cgccgtagcg ttagtgtctt tatcatcaca ggccgcaagc cccataacca
    20281 gcaggccaat tccgacatat ttaaccagat tgcgcattga cttcttcctt tcgataaacg
    20341 gccataacgg ctcattcatc catcttatca caactctgat aacgaacctt taactcgcct
    20401 gcaaagcgtt gatttcggat ttatctgcca gtcttttcaa cagcatattg agtaatacgc
    20461 catacattgg caggaagaaa acgatactga ttaacacttt gaaacagtaa tcgaccagcg
    20521 cgatttccat ccagtgttca gccataaagg catccgggct acgccagaag gcaatgaaga
    20581 agaaggccag cgtgtcgctg acgttaccga acagtgtgga cgctgtcggt gccagccacc
    20641 agtgacgact ctgacgcagg cggttaaaaa cgtgcacgtc gaggatctgc cccagcgcgt
    20701 aggccatgaa actggcggtg gcgatacggg cgacaaacag gttgaagtgg gcgagcgcgc
    20761 cgaatccttg ccatgacccc atataaaaca gcgatgagat gacgtaggag atcagcaatg
    20821 cggggatcat taccgcaaag atgatgcgac gagccagcgg cgcgccaaaa atacgcacgg
    20881 tcaggtcagt agcgagaaag ataaacggaa agctaaacgc gccccaggtg gtatgaaaac
    20941 caaaaatgga gaccggaagc tgaaccagat agttactgga ggtgatcacc agcagatgaa
    21001 ataacgataa ccagaacaac gccttatagc gttgcgtttg cgagaaaacg ttcatattgt
    21061 acctttttga ttaaccattg gggtgaggga acccaatacg tacgacacgt ctgttacctt
    21121 aacataataa cgtgctgacc cttttttcga gccgccgcat gatactgctt tacgttgaca
    21181 atgcaatggt taattttcac gcaatcgtta acctggtttg cttacggact cacagggcgt
    21241 aaactagcgc cgttttttta tgtgatgaga agaaaatgac cgatctcttt tccagccctg
    21301 accacacact cgacgcactt ggcctgcgct gcccggaacc agtaatgatg gtgcgcaaaa
    21361 cagtgcgcaa tatgcagcca ggtgaaacgt tgctgattat tgctgacgat ccggcgacga
    21421 cccgtgatat tcctgggttt tgtaccttta tggaacacga actggttgct aaagagacgg
    21481 atggactgcc ttatcgttat ttgattcgta aaggcggttg atgggggaag attggcttcg
    21541 atgccgcctt ttgccctcac cctaaccctc tccccagaga ggcgagggga ccgatcgcgc
    21601 tcaatgttgc tgcttgaaac cggtttgtag gccggataag gcgttcaagc tgcatctggc
    21661 gatacggtgc acaatgcctg atgcgacgct gtcgcgtctt atcatgccta cgagttcgat
    21721 gctctgtgta ggccggataa ggtgt
//
LOCUS       DADTFG010000083        21648 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.083, whole genome shotgun sequence.
ACCESSION   DADTFG010000083 DADTFG010000000
VERSION     DADTFG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.083"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(52..1485)
                     /locus_tag="J1138_002848"
     CDS             complement(52..1485)
                     /locus_tag="J1138_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBA8454618.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFCIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(1561..2613)
                     /locus_tag="J1138_002849"
     CDS             complement(1561..2613)
                     /locus_tag="J1138_002849"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBA8454619.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            2797..3750
                     /locus_tag="J1138_002850"
     CDS             2797..3750
                     /locus_tag="J1138_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8454620.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(3791..4786)
                     /gene="pgl"
                     /locus_tag="J1138_002851"
     CDS             complement(3791..4786)
                     /gene="pgl"
                     /locus_tag="J1138_002851"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBA8454621.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            4941..5759
                     /locus_tag="J1138_002852"
     CDS             4941..5759
                     /locus_tag="J1138_002852"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HBA8454622.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQNFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(5760..6818)
                     /gene="modC"
                     /locus_tag="J1138_002853"
     CDS             complement(5760..6818)
                     /gene="modC"
                     /locus_tag="J1138_002853"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBA8454623.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(6821..7510)
                     /gene="modB"
                     /locus_tag="J1138_002854"
     CDS             complement(6821..7510)
                     /gene="modB"
                     /locus_tag="J1138_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBA8454624.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(7510..8283)
                     /gene="modA"
                     /locus_tag="J1138_002855"
     CDS             complement(7510..8283)
                     /gene="modA"
                     /locus_tag="J1138_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8454625.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(8450..8599)
                     /locus_tag="J1138_002856"
     CDS             complement(8450..8599)
                     /locus_tag="J1138_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HBA8454626.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            8728..9516
                     /gene="modE"
                     /locus_tag="J1138_002857"
     CDS             8728..9516
                     /gene="modE"
                     /locus_tag="J1138_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBA8454627.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPINEATSLQQGQNVTAYFNADSVIIATLC"
     gene            9584..11056
                     /gene="modF"
                     /locus_tag="J1138_002858"
     CDS             9584..11056
                     /gene="modF"
                     /locus_tag="J1138_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBA8454628.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLEGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            11318..12334
                     /gene="galE"
                     /locus_tag="J1138_002859"
     CDS             11318..12334
                     /gene="galE"
                     /locus_tag="J1138_002859"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBA8454629.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            12344..13390
                     /gene="galT"
                     /locus_tag="J1138_002860"
     CDS             12344..13390
                     /gene="galT"
                     /locus_tag="J1138_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBA8454630.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGETYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            13394..14542
                     /gene="galK"
                     /locus_tag="J1138_002861"
     CDS             13394..14542
                     /gene="galK"
                     /locus_tag="J1138_002861"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBA8454631.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALFPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            14536..15576
                     /gene="galM"
                     /locus_tag="J1138_002862"
     CDS             14536..15576
                     /gene="galM"
                     /locus_tag="J1138_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBA8454632.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            15779..16531
                     /gene="gpmA"
                     /locus_tag="J1138_002863"
     CDS             15779..16531
                     /gene="gpmA"
                     /locus_tag="J1138_002863"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBA8454633.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16697..17749)
                     /gene="aroG"
                     /locus_tag="J1138_002864"
     CDS             complement(16697..17749)
                     /gene="aroG"
                     /locus_tag="J1138_002864"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBA8454634.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCTDVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLASAVKARRG"
     gene            18065..18445
                     /locus_tag="J1138_002865"
     CDS             18065..18445
                     /locus_tag="J1138_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454635.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSDGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            18559..19500
                     /gene="zitB"
                     /locus_tag="J1138_002866"
     CDS             18559..19500
                     /gene="zitB"
                     /locus_tag="J1138_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBA8454636.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(19497..20216)
                     /locus_tag="J1138_002867"
     CDS             complement(19497..20216)
                     /locus_tag="J1138_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBA8454637.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(20254..21297)
                     /gene="nadA"
                     /locus_tag="J1138_002868"
     CDS             complement(20254..21297)
                     /gene="nadA"
                     /locus_tag="J1138_002868"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBA8454638.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         5537 a         5648 c         5323 g         5140 t
ORIGIN      
        1 ggtgagcgaa cgcagacgca gcacatgcaa cttgaagtat gacgagtata tttacagcat
       61 gttccagccg atcagcatat tccaccacca cacgccaagg gtgatatgca ccaggaaggt
      121 taatatcgtc agtaccgcac cgaccaacca ccaggattta atatcgttgt aacccacgcc
      181 aaagatgacc ggacctgccg caccgccata gtgagtaacc atgccgccat aggagttaga
      241 gaacaacagt gccagcgcgg ttaacattaa cggtgcgccg gagacattcg ccagcatggc
      301 aaataccggt aacatggcaa cgatataggc actaccagaa gcgaagaaat aacgcacgat
      361 gatgctgagg aaaataataa cgaagaaagc aacgttaccg tgaccatcaa atgccaggtt
      421 atttttaaag acttcagcta accattcgaa gaatttaact ttcgataata aggagcttaa
      481 gccgataata ccgccgtacc agattaaggt attccagccg cctttatttt taaccacgtc
      541 ttcccaggta acgataccca gcagcagcat agttgccata acaacgattg ccacggtgga
      601 ttcatcaacc cccagagact tactgaaaat ccagcccagc agcgccagca caaagacacc
      661 gagcagcatt ttttcgcgga ttttcatcgg tcccagttcg gcaaggcccg ctttagcgat
      721 ggttttgtta tccaccttct taatttctgg tggatacatg gtgtaaatca ccagcggggt
      781 gaccagcagc ataatgatgc ccggcaatcc ggcggctaac gcccatccac cccagctaat
      841 ttgcaggtgc agaatgtcgt tgatcatttt cagcgccaga atgttccccg ccattgcggt
      901 aaagaacata tagctggtgg ttttggtgac catgtaaatg gacatcatca ggtaatgtcc
      961 aacacgacgc ggactttttt ccggttctga tcccaaagcc actgccacgc tgttgatgat
     1021 cggtaacacg atgccgcccg cacgcgcggt gttagacggt gttgccggag ccagtaccag
     1081 atcgagaaat accgtaacgt aacccagacc cagggtagtg ctaccaattt taccaatcag
     1141 cagataggca atacgtttac ctaagcctgt ggttacaaac gcggcgctta aagtaaacgc
     1201 agaaaacacc agccaggtgg taccggaaga gtaaccgctt aatacggcgg tggttttaaa
     1261 ttccccacct gataagttac ccaccacgac catcgatgcg gcgacggcaa ttaacagtac
     1321 gacaggttcc gggaaaggct tgataaccag ccccacaatg gccgccaggt aaatacaaaa
     1381 aagcacccac gccagttcgc ttaatcctgc cggagctggc ataaagccaa taatacatgg
     1441 gatcgctaat atcagaatta gcttccataa cgatttttta ttcatttaat atcctgttct
     1501 ttctgtatga ctcatacaga attcctttat tgagtatcat tatcacgaca acaaaatttt
     1561 tcagggaaga taaacttcac cggaaaatat ttttctggtc gtccgaataa cagatgcgcg
     1621 taacgtcgtg gcatcctgac cttcattact taaatgaacg tcgagcgcac cactggggtg
     1681 ttcaatattg atattgccgt atcctacaga agggacgatt tgtcgggtga cggtaccttc
     1741 caatgcacaa ctactggaaa tagcaatagc accggttatc gccaaagcgc gatggcaaga
     1801 atgcggcata aaataacgca cattaattgc cccgcctttc tgcgctggag aaataagtac
     1861 aggtttaggg ataaccatat tactgacatc gcctaagccc attgctttac ccgcttgtag
     1921 acggatagat tcaatgcggg ctaataatgc tttgtcggca tccagctctg ccggtaattc
     1981 atagcctgtt tttcccagat attcagcagg aataatgaca actggcatcg ccatatcgat
     2041 acaggtcacc gggacatcgt caaaataatc aatctgatta tccgtcggga agacttttcc
     2101 ggttttcgtt ccggcggcat tcaggaaagt gagcgcaacc ggtgcggcag tacccggtac
     2161 gccgtcaatt ctggcgctac cctcgtactc gacaacacca tttggcgttt gcacatcagc
     2221 ttcgataaac gtacccgtat tgacgttgcg gatacgtacg cgggtaactg gcgaagtcgc
     2281 tgcaatcaaa ccattttcaa tggcgaatgc cccaacgcca gacagcatat tgccgcagtt
     2341 aggcgtggta tcgacacgtt gctcatggac gataacttga gcaaacagat aatcgacatc
     2401 tgcacgcgga tcgctggagc ggctaataat ggcgacttta ctggtcagcg gattaccgcc
     2461 gccaataccg tcaatttcca gatcgttacc ggaacccata attgccatca atattttatc
     2521 gcgctgcgtt tgatcttcgg gtagatgttc cgctaacagg aacgcgcccc tcgaggttcc
     2581 acctcgcatc atcacgcagg gtattttttt cataataatg ccctcaattt aaaaacataa
     2641 cttattgcga tatggttaca ttaagggcaa agcatctcta atgactttag ctcaacgatt
     2701 aataacattt tttcattaat ttaagttggc taaatgcagt taaaattttt aactgccagt
     2761 cacccaaaat cgtcgcttcc attttccgga gtaataatga agcatgaatt atcaagtatg
     2821 aaggcatttg tcatactggc agagtccagt tcatttaaca atgctgctaa attactcaat
     2881 attacgcaac ctgcattaac gcgaagaata aaaaaaatgg aggaagattt acacattcaa
     2941 ctatttgagc gtacaactcg caaggttacg ttaacaaaag caggaaaaag gttgctccca
     3001 gaggcgcggg aattaataaa aaaattcgat gaaacgcttt ttaatattcg tgatatgaat
     3061 gcttatcatc gtggtatggt gacgttagcg tgtattccga ccgcagtatt ttatttttta
     3121 ccgctggcaa tcggtaaatt taatgagctg tatcccaata ttaaagtgcg gattctggaa
     3181 caaggcacga ataattgcat ggaatcggtg ctgtgtaacg aatcagactt tggcataaac
     3241 atgaacaacg tcacaaattc atccattgat tttaccccac tggtcaacga gccgttcgtg
     3301 ctggcctgtc ggcgtgacca tccattggcc aaaaagcagc ttgtagaatg gcaggagtta
     3361 gtgggttaca aaatgattgg cgtacgctcc tccagcggca accgactgtt gatagaacaa
     3421 cagctagccg acaagccctg gaagctggac tggttttacg aagtgcgtca tctttcgacg
     3481 tcattagggc tggtggaggc gggactgggg atttcggcgc ttcctggtct ggcaatgccc
     3541 catgcgccct attcttcgat tattggtatc ccactggtgg aacccgttat acgtcggaca
     3601 ttggggatta ttcgccgcaa agatgccgtg ctttctccgg cagctgaacg cttttttgcc
     3661 ttgctgatta atttgtggac tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc
     3721 cagagacatg cgttacagga gataggttaa tacaccggcg caggagagac tgcgccggtt
     3781 aaatcagcgg ttagtgcgcg ttgaccacta cccacattgg cccctgccca accgcatagc
     3841 ggcctttttc atgcagtagc ccctgctcgc caacaatttc gtataccgag atgtggtgag
     3901 atttttgccc ggcggcaatc agatacttgc cgctgtgatc aacattgaag ccgcgcggct
     3961 gggtttccgt tggctggaag ccttctttac tcaacacgct gccatcttcc gaaacgctga
     4021 aaacggtaat cagactggcg gtacggtcgc aggcgtataa atgacgacca tccggggtga
     4081 tatgaatatc agccgcccaa cgggtgtcgg agaagttttc cggcatcata tccagcgtct
     4141 ggacacattc gatattgccg tgcggatctt tcagttccca gacatccact gagctgttta
     4201 actcattgac gcaataggca tattgttcgt ttggatggaa taccatatgg cgtgggccgg
     4261 ccccttcaac ggtagtcact tccgcagggt cctgcgctac cagatgacca tcatcgctga
     4321 ccgtaaacag gcaaatgcga tcctgcttta atgccggaac ccacaacgta cggttgtccg
     4381 gtgagatatt ggcggaatgg caaccgtcca gcccctcgac cacatcgacg acgcccactg
     4441 gcaggccatc ttccagacgc gttacgctca cgttacccgc attgtaagaa ccgacaaaga
     4501 caaactgccc ctggtgatcg gtggaaatat gcgtcgggct acccggcagc gcagactctg
     4561 cagcaaaggt cagcgcgcca tcatccggtg caatacgata cgccagaaca cgaaactccg
     4621 gacggacacc aacgtagagg taacgtttgt ccgggctgac caccatcggc tgcacctgcc
     4681 ccggcacatc gactacctgt gtcagcgtca gtgcgccttc atgattcagg ttccagacgt
     4741 gaatttgctg gctctcaggg ctggcgatat aaactgtttg cttcatgaat gctcctttgc
     4801 atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa
     4861 aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg aaaattctta
     4921 accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct
     4981 tattgacccc gaaaaagacc ctgcttcctt catcgataga agccctggcc cgtgctcgcg
     5041 aagcaggcta ccgattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt
     5101 atcaggcgct ggcgctggat acacctgcta tttgctgtaa cggcacctat ttgtatgatt
     5161 atcatgcaaa aaccgtacta gaagcggacc caatgcctgt taacaaagcc ctgcaactca
     5221 ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtggatgat gcgatggttt
     5281 atgagcaccc gaccggacat gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg
     5341 aacagcgtcc gactttcaca caagtcgctt ctctggctga aacggcgcaa caagttaacg
     5401 ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagaat tttggtaagc
     5461 atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag gttgatattg
     5521 cacgcggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt
     5581 cgatggaaaa cgtcgtggcc ttcggcgata acttcaatga tattagtatg ctggaagccg
     5641 ctggtacagg cgtggcgatg ggcaacgccg atgacgcagt aaaagcgcgc gccaacattg
     5701 tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac ctgatttaat
     5761 caggcggtta tcgacacact tttaatttgc gcatacagcc acaggccagg tttgatcgcc
     5821 agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc gacttccagc
     5881 tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg caatacgtta
     5941 cgaatgctgg tttgctgcgg cggttgtaaa accagcgaga catcggaagc ctgaatgcgg
     6001 atgcgtagcg cagcttgcag cggttcgtcc agcttattga cccacaaatg ctgatcgccc
     6061 agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gtactgtcac tttcagaatg
     6121 ctactttgtt gctctttcgg tagccacgga ttcatcacgc tactacccca gacttcttcc
     6181 agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc tgccagatgg
     6241 aggatttcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc ccgcgtcaga
     6301 cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc cagcggttca
     6361 tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac gcgctgtttt
     6421 tcgcctccgg acaggctgcc tggtaaacga tcaagcaacg gttcaatgcc taaaagcgcc
     6481 accagcttat cgaactgatt gaccatgctt ttcgccatgc cgtagcgcaa attgccacgc
     6541 actttataat gcggaaacag ccgcgcatcc tgaaacacat agcctacgcg acgcttttct
     6601 ggcgtcaggc agataccttt atcggcgtca tttagtaccc gaccattgag gacaatccgc
     6661 cctttttgcg gacgtgtcag cccactgatg gcgttaatca gcgaggtttt tccggcacca
     6721 gagacaccaa aaatagcggt gatgccattg gcgggcagcg tttcattaat cgtcaggcaa
     6781 tggttgccca acgtctggga aaaattcagt tccagcatga ttagcgcccc gcccgctcac
     6841 ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc gcaatagaaa
     6901 taatacacag tctcgccgcg ccactttccc cgccaggggt ctggatcagg gtatacatgg
     6961 cagaaggaat ggtccgcgtt tcaccaggaa tgttcgacac aaaggtgatg gttgcgccaa
     7021 attcaccgag cgaacgagcg aatgccagta ccgtaccaac aataattccc ggcaacgtca
     7081 gcggtaaggt aatagtgaag aagacacgcc agcgcccggc gcccaacgtt cttgcggcct
     7141 gctccagttt gatatcaaca ccttccagcg ccagacgaat cgcccgcacc atcagcggaa
     7201 aagacatgac agcggcagcg agaaccgcac cgcgccagct aaacgcgaaa gaaagaccaa
     7261 accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact aataataggt
     7321 aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacactgtcg agcagagctt
     7381 tgcccggaaa cgtgcaacgt accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa
     7441 acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat tctggatcgg
     7501 tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgcag cctgcggccc
     7561 cttcagataa tcgtaaaatg ctttcactgt cgcattgtta tgcccttcca caacagcaac
     7621 cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt
     7681 gctggcaact gcgtcagaac cgtaaacaat acccagcggc gcttcattgc gttcgaccaa
     7741 cgccagcgcg ccacgaacat cttccgccgg agccagtttc ggggagagcg tatcccatgc
     7801 gcccagtttt tgcagtgctt ctttcgcata aatgccagca gggacatgtt ccggatcacc
     7861 aaccgcgagg cgaccgccat tcagcagcga agtccagttg gttttgctgt cgatggtgaa
     7921 atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga gcagtgtctg
     7981 gcgcgaagcc gtatcgatcg cttttttatc aaccgcataa tccatccatt tctgatcggc
     8041 agaaataaac agatccgcag gcgcacccgc ttcaatctga cgggccaaag tagatgacga
     8101 agcgaaagaa gaaactacat ccacgccttt ctctttttta tactgcgtag caatgtcctg
     8161 cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat ctgccagtgc
     8221 attgccagca acagcgaaag agagtgccgc cccggtaaac aagttcaacc atttacgagc
     8281 cattggtaac cccttaatgt aacgttatgt aggcgtcaat ataacggcaa gttcggaatt
     8341 ttcccagcag ttattgctaa cctttgtgag gtagatgaga aaaaatatcg gcagaaaaag
     8401 caggaagttg agaaaaagaa aatgcccgac taagcgggca ttcagggaat caatgatttt
     8461 gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaaaacttca ccaagaccgt
     8521 aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg aataccagac
     8581 tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt ctaaccgcta
     8641 agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca
     8701 caataagact tttgccagga tattgttatg caggccgaaa tccttctcac ccttaagctc
     8761 caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat tgcgctttcc
     8821 ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc
     8881 attaacgaga tgaatcagtt aagtgagcat attctggttg agcgcgcaac aggcggtaaa
     8941 ggtggaggcg gcgcagtact gacccgctat ggtcagcgac tgattcagct ctatgactta
     9001 ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc cctgccgctg
     9061 aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg taaccagtgg
     9121 ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga tgtcttactg
     9181 gctgatggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc acgtctgggg
     9241 ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg gattactcag
     9301 gacgaggcgg tcgcgcaaaa cgctgacaac caattaccgg gtattattag tcatattgag
     9361 cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac actgtgcgcc
     9421 acagtgccga taaatgaagc gacttctctt cagcaaggac agaatgtcac ggcctacttt
     9481 aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt tgttatgaaa
     9541 cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat
     9601 tttgcaaggc acgtttcgtc ttggcgacac aaaaacgctg caattgcctc ggctaacgtt
     9661 aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agcgggaaat cggcgctggc
     9721 ccgcgcgctg tcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc agttttccca
     9781 catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat ggcagcggaa
     9841 taacaccgat atgctcggcc ctggcgaaga tgacaccggg cgtaccacgg ctgagatcat
     9901 tcaggatgaa gtaaaggata ctccgcgttg catgcaactg gcgcagcagt tcggtattac
     9961 cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa aaaccctgct
    10021 gtgtcaggcg ctgatgtcgg agcctgactt gttgatcctt gatgaaccgt tcgatggcct
    10081 ggatgttgcc tcacgtcagc aactggctga gctactcgcc tcgttacatc agtccggtat
    10141 tactctggta ctggtgctca atcgcttcga tgagatcccg gaatttgtcc agtttgctgg
    10201 cgtgctggcg gattgcacgt tagcggaaac tggcgctaaa gaagaactgc tccaacaagc
    10261 actcgttgcg caactggcgc atagcgaact gcttgaaggt gtgcaaatgc cggagccgga
    10321 tgaaccttca gcacgtcacg ccttacccgc caacgaaccg cgcattgtgc tgaacaatgg
    10381 cgtggtttct tataacgatc gccccattct taataacctt agctggcagg tgaatccagg
    10441 cgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat taagcctgat
    10501 tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggac gacgtcgcgg
    10561 cagcggcgaa accatctggg atatcaaaaa gcatatcggt tacgtcagca gtagtttgca
    10621 tctggattac cgggtcagca ctactgtgcg taatgtgatt ctttctggct attttgattc
    10681 gattggcatt tatcaggccg tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga
    10741 tattctcggc attgataaac gcacggctga cgctcctttc catagtcttt cctggggaca
    10801 gcagcgtctg gcgctgatcg tccgtgcgct agtgaaacat ccgacgttgc ttattctcga
    10861 tgaaccacta caggggcttg atccgctcaa tcgccagctt atccgccgtt ttgttgatgt
    10921 gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg aagatgcgcc
    10981 tgcctgtatt acccatcgtc ttgagttcgt gccggacggt gacttttatc gctatgcgct
    11041 gacaaaaata aactgagtcg gtagtgctga ccgtgccgga ggcggcgtaa acgtgttatc
    11101 cggccaacgg tttgacgcat gcaggcatga aaccgcgtct ttttttcaga taaaaagcgc
    11161 aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa cggctaaatt
    11221 cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct
    11281 atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg ttaccggtgg
    11341 tagcggttac attggaagtc atacctgtgt gcaattactg caaaacggtc atgatgtcat
    11401 cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg
    11461 cggcaaacat ccaacgtttg ttgaaggcga tatccgtaac gaagcgttga tgaccgagat
    11521 cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag ccgttggcga
    11581 atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcaccc tgcgcctgat
    11641 tagcgccatg cgcgccgcta acgtcaaaaa ctttatcttt agctcctccg ccaccgttta
    11701 tggcgatcag cccaaaattc catatgttga aagcttcccg accggcacac cgcaaagccc
    11761 ttacggcaaa agcaagctga tggtggaaca gatcctcacc gatctgcaaa aagcccagcc
    11821 ggactggagc attgccctgc tgcgctactt caacccagtt ggcgcgcatc cgtcgggcga
    11881 tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg cccaggttgc
    11941 tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagacggtac
    12001 tggcgtgcgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga
    12061 aaaactggcg aacaagccag gcgtacacat ctacaacctc ggcgctggcg tgggcagcag
    12121 cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta attatcattt
    12181 tgcaccgcgt cgcgagggcg accttccggc ctactgggcg gacgccagca aagccgaccg
    12241 tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg
    12301 gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc
    12361 cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc tggtttcacc
    12421 gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc
    12481 tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa
    12541 ccccgattac accgggactt acgttttcac taatgacttt gcggccttga tgtctgacac
    12601 gccagatgcg ccagaaagca acgatccgct aatgcgttgc cagagcgcgc gcggtaccag
    12661 ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga gcgttgcggc
    12721 attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctggggg aaacgtaccc
    12781 atgggtgcag gtctttgaaa acaaaggcgc ggcgatgggc tgctctaacc cgcatccgca
    12841 cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag accgtctgca
    12901 aaaagaatat ttcgccgaac agaaatcgcc aatgctggtg gattatgttc agcgcgagct
    12961 ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg taccttactg
    13021 ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc ggatcaccga
    13081 tttgaccgac gcccagcgca gcgatttggc actggcgttg aaaaagctga ccagtcgtta
    13141 tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg cgccatttaa
    13201 tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgttgcgctc
    13261 cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagaaaccc agcgagacct
    13321 gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc
    13381 cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg
    13441 ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac
    13501 cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagctg
    13561 tgcgccacgc gatgaccgta aagttcgcgt aatggcagcc gattatgaaa atcagctcga
    13621 cgagttttcc ctcgatgcgc ccattgtcgc gcatgaaaac tatcaatggg ctaactacgt
    13681 tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg tggacatggt
    13741 gatcagcggc aatgtgccgc agggtgcagg gttaagttct tccgcttcac tggaagtcgc
    13801 ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac aaatcgcgct
    13861 taatggtcag gaagcagaaa accagtttgt aggctgtaac tgtggaatca tggatcaact
    13921 gatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct cactggggac
    13981 caaagcagtt tccatgccca aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg
    14041 taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt
    14101 cttccagcag ccagccctgc gcgatgtcac cattgaagag ttcaacgctg ttgcgcatga
    14161 actggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt
    14221 tgaagctgcc agcgcactgg agcaaggcga cctgaaacgt atgggcgagt tgatggcgga
    14281 gtctcatgcc tctatgcgcg atgatttcga aatcaccgta ccgcaaattg acactctggt
    14341 agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt
    14401 tggtggctgt atcgtcgcgt tgttcccgga agagctggtg cctgccgtac agcaagctgt
    14461 cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc
    14521 acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac
    14581 cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga ctggggtgcg
    14641 actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc gctgcttggc
    14701 tgcgccagcc cggaatgcta tcaggatcag gccgcatttc tgggggcctc tattggtcgt
    14761 tatgccaacc gcatcgccaa tagccgttat acctttgacg gtgaaaccgt gacgctttca
    14821 ccaagtcagg gcgtcaacca attgcatggc ggcccggaag ggttcgacaa acgtcgctgg
    14881 cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat
    14941 cagggtttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga cgataaccgt
    15001 atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac
    15061 gtctatttca atcttgacgg cgagcaatct gacgtgcgca atcacaagtt gcagattctg
    15121 gcggaagaat atctgccggt tgatgaaggc ggcattccgc acgacggcct gaaatctgtc
    15181 gccggaacct cttttgattt ccgcaacgcc aaaatcatcg ccagtgagtt tcttgccgac
    15241 gacgatcagc gcaaagtgaa aggttacgat cacgcgttct tgttacaggc caaaggcgat
    15301 ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac
    15361 accaccgctc cggctctgca attctactcc ggcaacttcc tcggcggcac accgtcgcgg
    15421 ggaaccgaac cttacgccga ctggcaaggg ctggctctgg aaagcgaatt tctgccggac
    15481 agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg cgaagagtat
    15541 tccagcctga cggaatatca gtttattgct cagtaatgtt aagccctcgt catgagggct
    15601 ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact caatattcac
    15661 aacaacctta cactgcgaca ctattttcgc tatggttatg cgtaagcatt gctgttgctt
    15721 cgtcacggca atataatgag aattattatc attaaaagat gatttgagga gtaagtatat
    15781 ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg
    15841 tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc
    15901 aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacacct ctgtgctgaa
    15961 acgcgctatt cataccctgt ggaatgtgtt ggacgaactg gatcaggcat ggctgcccgt
    16021 tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc
    16081 ggaaaccgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg gttttgcggt
    16141 gactccgccg gaactgacta aagatgatga gcgttatccg gggcacgatc cgcgttacgc
    16201 gaaactgagc gagaaagagc tgccgctgac ggaaagcctg gcgctgacca ttgaccgcgt
    16261 gatcccttac tggaatgaaa ccattctgcc acgtatgaag agcggtgagc gcgtcatcat
    16321 cgccgcacac ggtaactcgt tacgtgcgct ggtgaaatat ctcgataaca tgagcgaaga
    16381 agagattctt gagctgaata tcccgaccgg cgtgccgctg gtttatgagt ttgacgagaa
    16441 cttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg cgaaagcagc
    16501 ggcggttgct aaccagggta aagcgaagta aacgtcattc gtttaaaaat gagaaagccg
    16561 actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca ttgcctgatg
    16621 cgacgctgcg cgttttatca ggcctccggt gattaatcgg atacgccact ctgacggcgc
    16681 atccgacaat taaactttac ccgcgacgcg cttttactgc actcgccagt tgacgtaaca
    16741 gagcatcggt atcatcccag ccaatgcagg catcggtgat gctcttaccg taggccagcg
    16801 gttccccgct ctcgaggctc tgattgcctt ccaccagatg gctttccacc atcacgccaa
    16861 taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agtacaaaca tccatctgct
    16921 ttttgaattg ttttgacgag ttagcatggc tgaaatcgat catcacctgc gctggcaggc
    16981 ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt
    17041 taccgccgcg cagaatgata tggcaatcgc cgttaccgct ggtattcaca atcgccgaat
    17101 gcccccattt cgttacggac aggaagcagt gcggcgcacc ggcggcatta atggcatcga
    17161 tagccacttt aatcgtacca tcagtgccat ttttgaagcc taccgggcaa gaaagccctg
    17221 atgccagttc gcggtgcacc tgcgattccg tagtacgtgc gccaattgcg ccccagctca
    17281 tcaggtcagc gagatattgt ggggtgatca tatccaggaa ttcacccgcc gctggcagac
    17341 cgctgtcgtt aatatcgagc agcaatttgc gggcaatacg cagaccgtcg ttgatctgga
    17401 agctgttatc catatgcgga tcgttaatca gccctttcca gcccaccgta gtacgcggct
    17461 tttcaaaata gacgcgcatc acgatttcca gctcatcttg cagctcttca cgcagcgtca
    17521 gcaagcgagc ggcatactct ttagccgcga caggatcatg aattgagcat gggccaatca
    17581 ccaccaacag gcgatcatca ttacctttca ggattttatg gatcgctttt cgggcatggg
    17641 cgaccgtatt cgcggcattt tcagtagcgg ggaatttttc cagcaatgcg acaggaggaa
    17701 gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg cctgttccag
    17761 tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc gtcagaatgt
    17821 gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga
    17881 ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat
    17941 tatttcaaga ttctctgctg ggtatctttg cctggctgaa atcttaaacc attgccttat
    18001 attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca
    18061 tcctatgaaa atgacaaaac tggccacact atttttgact gccactctaa gccttgccag
    18121 cggtgccgca ctggctgccg atagcggagc gcaaagcaat aacggccagg caaacgccgc
    18181 agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga
    18241 taataacggg gtaaataccg gttccgacgg cacaatgctg catccggatg gttcttcaat
    18301 gaacaatgac ggcatgacca aagatgaaga gcacaagaac accatgtgta aagatggtcg
    18361 ctgtccggat atcaataaaa aggtacaaac tggtgatggc atcaacaatg atgtcgatac
    18421 caaaaccgac ggtaccacgc agtaaccctg cgaaatagcc aataatggga gagcggagtc
    18481 tctccctttt aatttttccc ttctaaaagc agtatgatgt cataaagtta agtgagaata
    18541 attaagggat gactcgtcat ggcgcactca cactcacaca cttcctcaca cctgccagaa
    18601 gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctcgta
    18661 gaagtcattg gtggttttct ttccggttct ctggcattgc tggccgatgc gggtcatatg
    18721 ttgaccgata ctgccgccct gcttttcgcc ctactcgcag tgcaattttc ccgtcgtcct
    18781 cccaccattc gccacacttt cggttggcta agattgacca cgctcgcggc gtttgtgaat
    18841 gccatcgccc tggtggtgat tactattttg attgtctggg aggcgataga acgcttccgc
    18901 actccgcgtc cggtcgaggg cggcatgatg atggcaattg ccgtggctgg gctgctggca
    18961 aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtgcga
    19021 gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg
    19081 ttgattatta tctggaccgg ctggacacct gctgacccca ttctctcaat actggtgtcg
    19141 cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gtgtgaatga attacttgaa
    19201 ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gcttgtgccg ggaaatcccg
    19261 gaagtccgca atgtgcacca tgtgcatgta tggatggtag gcgagaagcc ggtgatgacg
    19321 ctgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttagatca gatccaacac
    19381 tacctgatgg atcactatca gattgagcac gccactattc agatggaata tcaaccttgt
    19441 catgggccag actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa
    19501 tgggacagcg cgcgtgaacc tctttcgcgt gcgctgttga tccacatccg gctgccgttg
    19561 agggcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca
    19621 aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta
    19681 cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg
    19741 aaagcgtccg gctgtagttc aggcatcgca acctgtaatc ctaatgcttg catgatcatg
    19801 actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaatc
    19861 gaaacaacgc aaaccgccaa ccagcttagc gccttcggca atggcaacca gcgaattttc
    19921 aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta aatattcgcg
    19981 gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg
    20041 ccaaacaaag tgacgttgat caggccaaag aagtagttac tgatcttctc cagactggca
    20101 aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa
    20161 ccgcctgccc ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcgtt
    20221 cccccaaagc gtaatgtgac gccttattat caattatcca cgtagtgtag ccgcaaaatc
    20281 cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctttcatcaa cataaacctc
    20341 gtgattgctt ccttccagtt ctaatgcctc tgcgatggcc tgaaggccat tcatggccat
    20401 ccacgggcaa tgcgcgcagc tgcgacaggt tgcgccctca cccgcggttg gtgcttccag
    20461 taactcttta tctggcaccg cctgctgcat tttgtagaaa atgccccgat cggttgccac
    20521 gataagccgc tgatgtggca atgttttcgc agcagcgatc agttgactgg tggaaccgac
    20581 cgcatccgcc atctctacaa tagcttgcgg tgattctgga tgcaccagta tggcagcatc
    20641 cgggtattct tcttgcaagc gggttaacgc ctgagtctta aactcatcat gcacaatgca
    20701 ggcaccctgc cagcataaaa tgtctgcgcc cgtctgtttt tgcacgtaac gccccagatg
    20761 tttgtcaggt gcccagatga ttttttcacc caaactatcc agatgatcaa taagttcgac
    20821 ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc
    20881 gtagacgacg acagtacgat cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg
    20941 gcagccgaga tccagtgaac attcagcctg aagtgtcggc atcagaattg ttttttccgg
    21001 actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca acgtagaagc
    21061 gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc caccggtttc
    21121 ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg
    21181 ttcttttagt agacgtttta tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg
    21241 cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatca cgctcatctt
    21301 accatctcgt tctacaggct taacgttaaa accgacttaa gcgtgatatt cgctattatc
    21361 ggcgtaattt gttttatata ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa
    21421 ttacgtatga gatgccgaat gccacacttt gcgtcttatt cctccgacgt agagttgttt
    21481 acgcagcaaa cgcggagtat gtcatcaggg tcaagaattg aaattgagag atgcctgaat
    21541 agcaaaaaag cgcctttagg gcgctttttt gcattggtgg gtcgtgcagg attcgaacct
    21601 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgac
//
LOCUS       DADTFG010000084        21441 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.084, whole genome shotgun sequence.
ACCESSION   DADTFG010000084 DADTFG010000000
VERSION     DADTFG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.084"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            11..6118
                     /gene="irp2"
                     /locus_tag="J1138_002869"
     CDS             11..6118
                     /gene="irp2"
                     /locus_tag="J1138_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HBA8454639.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDKVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYQYPQQLPPPLPVTYRDYLQQPAIQSLNA
                     DSLAWWQAQLDDIPPAPALPLRCMPQDVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQVYESEGELRFNWDFVAALFPAGQVERQFEQYCTLLN
                     RMTEDENSWHLPLAALVPPVKQAEQGTERTSRVCPEHSQSHIAADESTVSLICDAFRE
                     VVGESVTPAQNFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALAAYLTSV
                     ATVEKTKRSRPVRRRQRRI"
     gene            6206..15697
                     /gene="irp1"
                     /locus_tag="J1138_002870"
     CDS             6206..15697
                     /gene="irp1"
                     /locus_tag="J1138_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HBA8454640.1"
                     /translation="MDNLRFSSAPTADSIDASIVQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYHHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLEQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQSHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRFAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLSVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            15694..16794
                     /gene="ybtU"
                     /locus_tag="J1138_002871"
     CDS             15694..16794
                     /gene="ybtU"
                     /locus_tag="J1138_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HBA8454641.1"
                     /translation="MMPSASPKQRILIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            16857..17594
                     /gene="ybtT"
                     /locus_tag="J1138_002872"
     CDS             16857..17594
                     /gene="ybtT"
                     /locus_tag="J1138_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HBA8454642.1"
                     /translation="MCPFAGGSSSAFRHWQAEQLADCALSLVTWPGRDRLRHLEPLRS
                     ITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFETCRLLEQRGLAPQGLIISGCHA
                     PHLHSERQLSHRDDADFIAELIDIGGCSPELRENQELMSLFLPLLRADFYATESYHYD
                     SPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLSHVTGPVVIDGDHFYPIQQARSF
                     FTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            17598..19175
                     /gene="ybtE"
                     /locus_tag="J1138_002873"
     CDS             17598..19175
                     /gene="ybtE"
                     /locus_tag="J1138_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HBA8454643.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            19306..21327
                     /gene="fyuA"
                     /locus_tag="J1138_002874"
     CDS             19306..21327
                     /gene="fyuA"
                     /locus_tag="J1138_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HBA8454644.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKNNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         4332 a         6561 c         6180 g         4368 t
ORIGIN      
        1 aggaggaaga atgatttctg gcgcaccatc tcaggattcg ctgttaccgg acaaccgcca
       61 cgcggctgat taccaacaat tacgcgagcg gctcatacag gaactgaatt taacgccgca
      121 gcagttacat gaagagagca acctgatcca ggccggcctg gattccataa gattgatgag
      181 atggttacac tggtttcgta aaaatggcta ccgccttacc cttcgcgagc tgtatgccgc
      241 ccccacgctg gcggcatgga accagttaat gctcagccgg tcgccggaaa acgcggaaga
      301 agaaacgccg cccgacgaat catcctggcc gaacatgacc gaaagtaccc ccttcccatt
      361 gacgccagta cagcacgcct acctgacggg ccgcatgccg gggcagacgc ttggcggcgt
      421 gggttgccac ctgtatcagg agtttgaagg ccattgtctg acggcgtcgc agctggagca
      481 ggccatcacg accttgctgc aacgccaccc aatgctgcat atcgcctttc gccccgacgg
      541 gcagcaggtc tggctaccgc aaccttactg gaacggcgtc accgttcatg atttacgcca
      601 taacgacgct gaaagccgcc aggcctatct ggacgcactg cgccagcgcc tgagccaccg
      661 tcttttacgc gtggaaatcg gcgaaacgtt tgattttcag ctgacgctct tgccggacaa
      721 tcgccaccgc ctccatgtca atattgacct gctgattatg gatgcctcca gctttacgct
      781 tttcttcgat gagcttaacg ccctgctggc cggagaatcg ctgccggcta tcgacacccg
      841 ctatgatttc cgctcgtatt tgctgcacca gcagaagatc aatcaaccac tgagagacga
      901 cgcgcgcgct tactggctgg cgaaagcatc gacgcttccc cccgcgcccg tcttgccgct
      961 ggcctgcgaa cccgccacgc tacgtgaagt ccgtaatacc cgacgccgca tgattgtccc
     1021 ggcaacacgc tggcacgcct ttagcaaccg ggccggcgag tatggcgtga cgccgacaat
     1081 ggcactggcg acctgttttt ctgccgtgct ggctcgctgg ggcggcctga cgcgtctgct
     1141 gcttaacatc accttattcg accgccagcc gctgcacccg gcggttggcg cgatgcttgc
     1201 cgacttcacc aatattcttc tgctggatac cgcctgcgat ggcgataccg tcagcaacct
     1261 ggcgcgtaaa aaccagctca cgtttacgga ggactgggag catcgccact ggtccggtgt
     1321 cgaattactc cgtgaactca aacgccagca gcgctacccc cacggcgccc cggtggtatt
     1381 taccagcaat ctggggcgtt ccctctacag cagccgcgca gaatcgccgt tgggcgagcc
     1441 ggaatggggc atctcgcaaa cgccgcaggt ctggatagat catctggcgt tcgagcatca
     1501 cggcgaggtc tggctacaat gggacagcaa cgacgcgctg ttccctccgg cgttagtcga
     1561 gacattgttc gacgcctact gccagttgat taaccaactc tgcgatgacg aaagcgcctg
     1621 gcaaaagccg ttcgcagata tgatgcccgc cagccagcgc gcgatacgcg aacgggtcaa
     1681 cgccaccggc gcccccattc ccgaaggctt gctgcatgaa ggcattttcc gtatcgctct
     1741 gcaacagccg caggcgctgg cggtaacgga catgcgttat cagtggaatt atcatgagct
     1801 gacagactat gcccgccgtt gcgcgggcag gttaatcgag tgcggggttc agcccggcga
     1861 taatgtggct atcacgatgt cgaaaggcgc aggacaactt gttgcggttc tggccgtcct
     1921 gctggccggg gcggtttacg ttccagtttc gctggatcag cctgccgcac ggcgcgagaa
     1981 aatctacgct gacgccagcg tccggctggt gctcatttgt cagcacgacg ccagcgccgg
     2041 gtcagacgat attcccgccc ttgcctggca gcaggccatt gaggcggagc cgatcgccaa
     2101 cccggtagta cgcgccccca cgcaaccggc ctacattatc tacacctccg gctctaccgg
     2161 tacgccgaaa ggggtagtca tttctcaccg gggagcgctt aacacctgtt gcgatatcaa
     2221 tacccgctat caggttggcc cgcatgacaa ggtgctggcc ctctccgccc tacattttga
     2281 tttatcggtt tacgacattt ttggcgtact gcgcgcgggc ggcgcgctgg tgatggtgat
     2341 ggaaaatcaa cggcgcgatc ctcacgcatg gtgtgagctg atccagcgcc atcaggtcac
     2401 gctctggaac agcgtcccgg cgctgttcga tatgctgctg acctggtgtg aaggtttcgc
     2461 cgacgccacg ccggaaaacc tgcgcgcagt gatgctttcc ggcgactgga tcgggcttga
     2521 cctccccgcc cgttatcggg ccttccggcc acaaggacaa tttatcgcga tgggcggcgc
     2581 caccgaggcg tctatctggt ctaacgcctg cgaaattcac gacgtccctg cccactggcg
     2641 ctccatccct tacggttttc cgctaaccaa ccaacgctac cgggtggtgg atgaacaggg
     2701 ccgggactgc cctgactggg tgccgggtga attatggatt ggcggcattg gggtcgcgga
     2761 aggctatttc aacgatcccc tgcgtagcga gcagcaattt ttgacgctcc cggacgagcg
     2821 ctggtatcgc accggcgatc tcggctgcta ctggccagat ggcacaatcg agttcctcgg
     2881 tcgtcgcgac aagcaggtca aagtcggagg atatcgcatc gagctgggcg aaatcgaaag
     2941 cgcgctcagc cagctggcgg gggtgaaaca agcaaccgtt ctggcgatcg gcgaaaaaga
     3001 aaaaacgctg gcggcatacg ttgttcctca gggcgaggct ttttgcgtta ccgatcatcg
     3061 gaacccggca ctgccgcagg cgtggcacac gcttgcggga acgttgccct gttgcgccat
     3121 ctcgccagag atctccgcag aacaggtagc cgatttcctt cagcatcgcc tgctaaaact
     3181 gaagccgggt cacaccgctg gcgccgatcc tctccccctg atgaactcac tcgctatcca
     3241 gccgcgctgg caggccgtgg tggaacgctg gttagcattt ctggtgacac aacggcgact
     3301 gaagcccgct gctgaaggtt atcaggtctg cgctggtgaa gaacgcgagg atgagcaccc
     3361 gcacttcagc ggacatgatt taacgttatc gcaaattctt cgcggtgccc gtaacgaact
     3421 gtcgttactg aacgacgcgc agtggtcccc ggaaagcctg gcctttaacc atccggccag
     3481 cgccccgtat attcaggaac tggcgacaat ttgccaacag cttgcacagc gcttacagcg
     3541 cccggtacgc ctgcttgagg tgggaacccg cactggccgc gccgcagaat cgctgttagc
     3601 acagctcaac gccggacaga ttgagtatgt cgggcttgag cagagccagg agatgctgct
     3661 gagcgcccgg cagaggctcg ccccctggcc tggcgcccgt ctgtccctct ggaatgcaga
     3721 cacgctggcg gcgcacgctc actcggcgga cattatctgg cttaataacg ccctgcatcg
     3781 tctgctgccg gaagatcccg ggctccttgc gacattacaa cagcttgccg ttcccggcgc
     3841 gctgctctac gtgatggagt ttcgccagtt aacgccgtcc gccctactca gcacgctcct
     3901 gttaaccaat gggcagccgg aggccttgct gcataacagc gccgactggg cggcattatt
     3961 tagcgcggcc ggcttcaact gtcagcatgg cgatgaggtc gcggggttac aacgcttcct
     4021 cgtacaatgt cctgacaggc aggtgcgccg cgatccccgt caacttcagg ccgccctcgc
     4081 cgggcgtctg ccggggtgga tggtgccgca acggatcgtc ttcctcgacg ccttaccgct
     4141 gacggctaac gggaaaattg actaccaggc gctgaagcgt cgtcataccc ctgaagcgga
     4201 aaacccggcc gaagcggatt taccccaggg cgacattgaa aaacaggttg ccgccctctg
     4261 gcagcaactc ttatcaactg gcaatgtcac cagagaaacc gacttcttcc agcaaggcgg
     4321 cgatagcctg ctggcgaccc gtctgaccgg gcaacttcat caggcaggtt atgaagcgca
     4381 attaagcgac ctgtttaatc atccccggct ggcggatttt gccgccacgc tgcggaaaac
     4441 cgacgtcccg gtcgaacaac cattcgtcca ctcccctgaa gatcgctacc agccctttgc
     4501 gcttaccgac gtgcagcagg cttacctggt ggggcgtcag ccgggctttg ccctgggcgg
     4561 cgtcggctca catttctttg ttgaatttga aattgccgat ctggatctca cccggttgga
     4621 gacggtctgg aaccgattaa tcgcccgcca cgatatgcta cgcgccgtcg tgcgtgatgg
     4681 acagcaacag gtgctcgaac agacgcctcg ctgggtaata cccgcacaca tcctccatac
     4741 gcctgaagag gcgttgcagg tgcgcgaaaa actggcccat caggtactca accccgaagt
     4801 ctggccggta ttcgatctcc aggtcggtta cgttgacggg atgcccgccc gcctgtggct
     4861 gtgtctggat aacctgttac ttgacgggct gagcatgcag attctgttgg cggagctgga
     4921 acatggctac caatatccgc aacagttgcc tccgccgcta cccgtcacct acagggatta
     4981 cctgcaacaa cccgcgatcc agtcgcttaa cgcagattct ctggcatggt ggcaggcgca
     5041 acttgatgat attcctccgg ctcctgcatt gccgctacgt tgcatgcctc aggacgtcga
     5101 aacaccgcgc ttcgcccgcc tgaacggcgc gctggacagc acgcgctggc atcggctgaa
     5161 aaaacgggcg gctgacgccc atctcacccc gtcggccgta ctgttgtcgg tgtggtcaac
     5221 ggttctctct gcatggagtg cacagcctga gttcacgctt aaccttacgc ttttcgacag
     5281 gcgaccgctg cacccgcaaa tcaaccagat tctgggcgat ttcacctcgc tgatgctgct
     5341 gagctggcat cccggcgaaa gctggctgca cagcgcgcag tcactacagc agcggctgag
     5401 ccagaacctc aaccaccgcg atgtgtcagc catccgcgtg atgcgtcaac tggcgcaacg
     5461 gcaaaacgtg cctgccgttc cgatgcccgt cgtctttacc agcgcgctgg gctttgagca
     5521 ggataacttc ctcgcccggc gtaatctgct caaaccggtc tggggcatct cccagacgcc
     5581 gcaggtctgg ctcgatcacc aggtttatga atccgaaggc gaactgcgct ttaactggga
     5641 ttttgtcgcc gcgctgtttc ctgccgggca ggtggaacgt cagtttgagc agtattgcac
     5701 cttgctaaac cgaatgaccg aggatgaaaa cagctggcat ttgccactcg ccgcgctggt
     5761 gcctcccgtt aagcaggcgg agcaaggtac agagcgcaca tcgcgcgtat gccctgagca
     5821 ctctcagtca cacattgcgg cggacgagag caccgtcagc ctgatttgcg acgccttccg
     5881 cgaggtggtt ggcgagtctg tcacgcccgc acaaaacttc tttgaggcgg gcgcaacgtc
     5941 gctgaatctg gtgcaactgc acgttttgtt acaacgtcac gaattttcga ctctgacgtt
     6001 acttgacctc tttacccacc cttctcctgc agccctggcc gcttatctga ccagtgtcgc
     6061 cacggtggag aaaaccaaac gctctcgccc tgttcgccgt cgtcagcggc ggatatagcg
     6121 cgaagcaaac tgattttccc cggaacgcca tcgcgaacgc atggcgttcc attgacttta
     6181 tgaaccttag gaaatgggac cgattatgga taacttgcgc ttctcttctg cgccaacagc
     6241 agattccatt gatgcatcga tcgttcaaca ctacccggac tgcgaacctg tcgcggttat
     6301 cggctacgcc tgccattttc ctgaatcgcc ggatggcgaa acgttctggc aaaatctgct
     6361 ggaaggtcgt gaatgcagcc gacgctttac gcgcgaagag cttctggccg tcggtctgga
     6421 tgccgccatc attgacgatc ctcattatgt caatatcggt acggtgttag acaacgccga
     6481 ctgcttcgac gccaccctgt ttggctattc gcgacaggaa gcggagtcga tggacccgca
     6541 gcagcgcctg tttttgcagg cggtctggca tgcgctggaa catgccggtt atgcccccgg
     6601 cgccgtcccc cataagaccg gcgttttcgc ctcttcccgg atgagtacct accccggtcg
     6661 cgaagcattg aacgtgacag aagtcgcgca ggtaaaaggt ctgcaatctc tgatgggcaa
     6721 tgataaagac tatattgcca cccgcgccgc gtacaaactc aacctgcacg gcccggcgtt
     6781 atcggtacag accgcctgct ccagctcgct ggttgccgtg catctggcct gtgaaagcct
     6841 gcgcgcaggc gaatccgata tggcggttgc cggcggcgtg gcgctctctt tcccccagca
     6901 ggcaggctac cgctaccagc ccggaatgat tttctctcct gatggtcact gtcgtccctt
     6961 tgacgcctcg gctgagggca cctgggccgg taacggtctc ggctgcgtgg tgctgcgtcg
     7021 cctgagagac gcgctgctgt caggcgatcc gattatctcg gtgatcctct ccagcgcggt
     7081 caacaacgac ggcaacagaa aggtcggcta taccgcccct tccgtcgcag ggcaacaggc
     7141 agtcatcgaa gaggcgttaa tgctggcggc catcgacgac aggcaggtag gttacattga
     7201 aacccacggc accggcacac cgctgggcga cgcgattgaa attgaagcgt tacgcaacgt
     7261 ctatgcgcct cgcccgcagg atcagcgctg tgcgctcggt tccgtgaaaa gtaacatggg
     7321 ccatctggat accgcggcgg gcattgccgg actgctgaaa accgttctgg cagtcagtcg
     7381 cgggcaaatt cctcccttac tgaattttca cacccccaac ccggcgctga aacttgaaga
     7441 gagccccttt accataccgg tgtcggcaca ggcatggcag gacgaaatgc gctatgcggg
     7501 cgtctcctcc tttggtattg gcggcaccaa ctgccatatg atcgtcgcct cgctgcccga
     7561 cgcgctcaac gcgcgcctcc ccaatacgga tagcggcaga aaaagtaccg cgctgctgct
     7621 cagcgccgcc agcgacagcg cgttgcggcg gctggcgacg gattatgccg gggcgctgag
     7681 agagaatgcg gatgccagct ctctggcctt cacagccctg cacgcgcgcc gtctcgatct
     7741 ccccttccgc ctggcggcgc cattaaaccg tgaaaccgcc gaggcgctca gcgcctgggc
     7801 cggtgagaaa tcgggggcgc tggtttacag cggccacggc gccagcggca agcaggtgtg
     7861 gctgtttacc ggccagggct cgcactggcg cactatgggt caaacgatgt accatcactc
     7921 aacggcgttt gccgacacgc tggatcgctg tttttccgcc tgtagcgaaa tgctcacgcc
     7981 gtcactgcgc gaagcgatgt ttaaccccga ttcggcgcag ctagacaata tggcctgggc
     8041 gcagccggcg attgtcgcgt ttgaaatcgc gatggcggcg cactggcgtg ctgaaggact
     8101 gaagccagac ttcgccattg ggcattccgt cggtgaattt gccgctgccg ttgtctgcgg
     8161 acactatacg attgaacagg tcatgccact ggtttgtcgg cgcggcgcgc taatgcagca
     8221 gtgcgcaagc ggcgcgatgg tggcggtatt tgcagacgaa gacacgctga tgccgctggc
     8281 tcgccagttt gagctggatc tcgccgccaa caacggtacg caacatacgg tattttccgg
     8341 gccggaagcc cgtctcgcgg tattttgcgc cacgctctcg cagcatgaca ttaactatcg
     8401 tcgcctgagc gtaaccggtg cggcgcactc cgctttactg gagccgatac tcgatcggtt
     8461 ccaggacgcc tgcgcgggac tgcacgcgga gccggggcaa ataccgatta tttccacgct
     8521 caccgccgac gtcattgatg agtcaacgct caaccaggcg gattactggc gccgacacat
     8581 gcgccagccg gtgcgtttta tccagagtat tcaggtggcg catcagctcg gcgcccgcgt
     8641 ttttctggag atggggcccg atgcccagtt ggttgcttgc gggcagcgcg aataccgcga
     8701 taacgcatac tggatagcca gcgcccggcg taacaaagag gcgagcgatg tcctcaatca
     8761 ggccctgctc cagctttacg ctgccggcgt cgccctaccg tgggccgacc tgctggcggg
     8821 cgatggacaa cgtatcgctg cgccatgtta tccgtttgat actgaacgtt actggaaaga
     8881 gcgcgtctcc ccggcctgcg agcctgccga cgcagcgctg tctgccgggc tggaggtggc
     8941 gagtcgcgcc gcgacagcgc tcgatctccc tcgcctggaa gcgcttaaac agtgcgccac
     9001 gcgactgcac gccatctacg tcgatcaact ggtacaacgc tgtaccggcg atgccattga
     9061 gaacggcgtg gacgccatga ccatcatgcg ccgtggacgt ctgctgcccc gctaccagca
     9121 gctactccag cgcctgctga ataactgcgt ggtcgacggc gattaccgct gcaccgacgg
     9181 gcgatacgtc cgcgcccgcc ccattgaaca tcaacagcgg gaatcactgc tgacggaact
     9241 tgccggttat tgtgaaggtt ttcaggctat tcccgacacc atcgcccgtg ccggcgatcg
     9301 gttatatgaa atgatgagcg gcgcggaaga accggtggcg attatcttcc cgcaaagcgc
     9361 ctccgacggc gtggaagtgc tgtatcagga attcagcttt ggccgctatt tcaaccaaat
     9421 cgccgccggg gtattacgcg gcattgtcca gacgcgtcag ccccgccagc cgttgcgtat
     9481 tcttgaggtt ggcggcggaa ccggcggcac caccgcgtgg ctgctgccgg aactcaacgg
     9541 cgttccggcg ctggagtacc atttcaccga tatctcggcg ctgttcaccc gccgcgccca
     9601 gcagaaattc gccgactatg attttgtgaa gtatagcgag ctggatctcg aaaaagaggc
     9661 gcagtctcag ggtttccagg cacagtctta cgatcttatc gtggcggcga acgtaattca
     9721 cgccacccgc catattggcc gcacgctcga taatctgcgc cccctgctca agccgggcgg
     9781 gcgcctgctg atgcgcgaaa tcacccagcc aatgcgtctg tttgacttcg ttttcggccc
     9841 gctggttctt ccgctacagg atctcgacgc ccgcgaaggt gagttattcc tcaccaccgc
     9901 tcagtggcaa caacagtgcc gccacgccgg attcagcaaa gtggcgtggc taccgcagga
     9961 tggcagcccg accgccggga tgagcgaaca tatcattctc gccacgctgc ccggtcaggc
    10021 ggttagcgcc gtaacattca ccgcgccatc agaacccgtg ttggagcagg cgctgacgga
    10081 taacggtgat tatctcgccg actggtctga ttgcgcaggt cagcccgaac ggtttaacgc
    10141 ccgctggcag gaggcctggc gtctgctttc acagagtcat ggcgacgctc tgcctgtgga
    10201 accgcccccc gtcgccgccc cggagtggct ggggaaggtt cgcttaagct ggcaaaacga
    10261 agccttttcc cgcggtcaga tgcgcgttga agcccgtcat cctgctggcg agtggctgcc
    10321 gctatcgccc gccgcgcctc ttcctgcgcc gcaaacgcat tatcaatggc gctggacgcc
    10381 cctcaacgtc gccagcattg accatccgct tacctttagc ttcagcgccg gtacgcttgc
    10441 gcgcagcgac gagctggcgc aatacggcat cattcacgat ccgcacgcct cttcgcgact
    10501 gatgattgtt gaggagagcg aggatacgct ggccttagcg gagaaagtga tagcagcgct
    10561 caccgccagc gcagccggat tgattgtggt tactcgccgc gcgtggcgag tcgaggaaaa
    10621 tgaagcactc tctgcatccc atcacgcgct atgggccttg cttcgcgtcg cggccaacga
    10681 acagccggaa cggttgcttg ccgccatcga tctcgccgaa aacaccccgt gggaaacgct
    10741 gcatcaaggg ttgagcgcag tctcactatc acagcgctgg ctcgccgcac ggggtgacac
    10801 cctttggctc ccttcactag cgcccaatac gggatgcgcc gctgaattac cggcaaacgt
    10861 gtttaccggc gatagccgct ggcatctggt gaccggagcg tttggcggat taggccgctt
    10921 tgccgtgaac tggctcagag aaaaaggggc gcgacgcatc gccctgctgg cgccgcgcgt
    10981 ggatgagtca tggctacgcg acgtggaggg cgggcagacg cgcgtctgcc gttgtgatgt
    11041 gggcgatgcc gggcaactgg ccacggttct tgacgatctg gcggccaacg gcggcattgc
    11101 cggagcgatt catgccgctg gcgtattggc tgacgcgccc ttgcaggagc ttgatgacca
    11161 ccagctggct gccgttttcg cggtaaaagc gcaggcggca agccagctgt tgcaaaccct
    11221 gcgcaaccac gacggacgct atcttattct ctactcttcc gctgccgcca ccctcggcgc
    11281 gccgggtcag agcgcccatg cgctggcctg cggctacctg gacgggctgg cccagcagtt
    11341 ttccaccctt gatgcgccga aaacgctctc tgtcgcctgg ggcgcatggg gagaaagcgg
    11401 tcgggcggcc acgccggaaa tgctggcgac gctcgccagc cgaggtatgg gcgcgttaag
    11461 cgatgccgaa ggctgctggc acctggaaca ggcggtgatg cgcggcgccc cgtggcgact
    11521 ggcgatgcgc gtttttaccg acaaaatgcc cccgttacaa caggctctgt ttaacatcag
    11581 cgccacagaa aaagccgcaa cgccggtcat tcctcctgct gatgacaacg cctttaacgg
    11641 cagcctgagc gatgaaacag cggtgatggc atggctgaaa aagcggattg cggttcagct
    11701 aaggctgagc gatccggcgt cactgcatcc aaaccaggat ctgttgcaac tcggcatgga
    11761 ctcgctgctc ttccttgaac tcagtagcga tattcagcac tacctgagtg tacgcatcaa
    11821 tgcggaacgg gcgtggcagg atctgtctcc tcatggactc acgcagctta tctgttctaa
    11881 gccagaggcg acgcctgccg cttcgcagcc ggaagtattg cggcacgacg ccgacgagcg
    11941 ttatgcgccc ttccctttga cgcccattca gcacgcctac tggctggggc gaacccacct
    12001 cattggctat ggcggcgtcg cctgtcacgt cctgtttgag tgggataaac gccacgatga
    12061 gttcgatctc gccatactgg agaaagcatg gaaccagctc atcgcacgcc acgatatgtt
    12121 gcgtatggtg gttgatgccg acgggcagca gcgaatcctg gcgacaacgc cggagtatca
    12181 catcccgcgt gacgatctgc gcgcgctttc cccggaagaa cagcgcatcg cgctggaaaa
    12241 acggcggcat gaactgagct atcgcgtttt gcctgccgac cagtggcctc tttttgagct
    12301 ggtggtcagc gaaatcgacg attgccatta ccgtctgcat atgaacctcg accttttgca
    12361 gtttgatgtg cagagtttta aagtcatgat ggacgacctg gcgcaggtct ggcgcggtga
    12421 aacgctggcg ccgctcgcta ttaccttccg tgattatgtg atggctgaac aggcgcgccg
    12481 acagacatcg gcatggcacg atgcctggga ttactggcag gaaaaactgc cgcaactgcc
    12541 cttagcgcca gagctgccgg tggttgagac gcccccggaa acgccacact tcaccacctt
    12601 caaatcgacg atcggcaaga cagaatggca ggccgtgaaa cagcgctggc agcagcaagg
    12661 cgtcacaccg tctgccgcgc tgctcacgct gtttgccgcc acccttgagc gctggagccg
    12721 taccacaaca tttacgctga acctgacgtt cttcaatcgc cagccgatcc atccgcaaat
    12781 caaccagttg attggtgatt ttacctccgt cacgctggtt gattttaact tctcagcgcc
    12841 ggtgacgttg caagagcaga tgcaacagac ccaacagcgc ctctggcaaa acatggcgca
    12901 cagtgaaatg aacggtgttg aggtgatccg tgagctgggc cgcctgcgcg gatcacaacg
    12961 tcaaccgctg atgccggtag tgtttaccag tatgctgggg atgacgctgg aaggcatgac
    13021 tatcgatcag gcgatgagcc atctgttcgg cgaaccctgc tatgtattca cgcaaacgcc
    13081 gcaggtctgg ctggatcatc aggtcatgga gagcgacggc gagttgatgt ttagctggta
    13141 ctgcatggac aacgtgctgg aacccggcgc tgccgaggcg atgtttaatg actattgcgc
    13201 catcctgcaa gccgtcatcg ccgcccctga aagcctgaag actctcgcca gcggcatcgc
    13261 cgggcacatt ccccgccgac gctggccgct gaacgcgcag gcggactacg acctgcggga
    13321 tattgagcag gcgacgctcg aataccccgg catccggcag gccagagcgg aaataaccga
    13381 acagggcgcg ttgacgctgg atatcgtgat ggccgacgat ccttcgccat cagcggcgat
    13441 gcctgatgag cacgaactta cccaactggc gctgccgttg cctgagcagg cgcagcttga
    13501 tgagctggag gcgacctggc gctggctgga ggcgcgtgcg ctacagggga tcgcggctac
    13561 gctaaatcgt cacggcctgt ttaccacgcc ggagatcgcc catcgcttta gcgcaatagt
    13621 acaggcgctg tccgcgcaag cgtctcacca gcgtctgctg cgccagtggc tacagtgtct
    13681 gacggaaaga gagtggttaa tccgcgaagg tgaaagctgg cgctgccgca ttccgctcag
    13741 cgagattcct gagcctcagg aagcgtgccc gcaaagccaa tggagccagg cgctggcgca
    13801 gtatctggaa acctgcatcg cccggcacga cgccctcttc tccgggcagt gttctccgct
    13861 ggaattgctg ttcaacgagc agcatcgcgt taccgacgcg ctgtatcgcg acaaccccgc
    13921 cagcgcctgt ctgaatcgct ataccgcgca gattgccgcc ttgtgcagcg cagaacggat
    13981 tctggaggtt ggcgccggaa ccgcagccac taccgcgccg gtgctgaagg ccacgcggaa
    14041 cacgcggcag tcgtaccact tcacggacgt ctccgcgcag ttcctcaatg acgccagagc
    14101 ccgtttccat gatgaatcgc aggtgtctta tgccttgttc gacatcaacc agccgctgga
    14161 tttcaccgcc cacccggagg cgggttacga cctgatcgtt gccgtcaatg tgctccacga
    14221 cgccagccat gtcgtccaga cgttgcgcag attaaaactg ttgctgaaag ccggcggacg
    14281 tttgctgatc gttgaagcga cggagcgaaa cagcgtattc cagctggcga gcgtgggctt
    14341 tattgaggga ttaagcggat accgcgattt ccgccgccgg gatgagaaac cgatgctcac
    14401 ccgctccgca tggcaggagg ttctcgttca ggccgggttt gcaaacgagc tggcgtggcc
    14461 cgcgcaggaa tcgtcgccgc tgcgccagca tctgctggta gcacgttcgc ctggcgtaaa
    14521 tcgcccggat aaaaaagccg tgagccgcta tttacagcag cgctttggca ccggtctgcc
    14581 cattttacag atccggcaaa gagaagcgtt atttacgccg ctgcatgccc cgtctgatgc
    14641 gccgactgag ccagccaaac ccacgccagt tgccgggggg aatccggcgc tggaaaaaca
    14701 ggtggctgaa ctctggcaat cgctgctgtc tcgccccgtg gcaaggcatc acgacttttt
    14761 cgaactgggc ggcgacagcc tgatggcgac aaggatggtc gcgcagctaa accggagagg
    14821 gattgccagg gctaaccttc aggatctgtt cagccattcg acgctgagcg acttctgcgc
    14881 ccatctacag gcggctacgt caggagagga caacccgata cccctttgcc agggcgacgg
    14941 tgaggaaacc ctgtttgtct tccacgcttc agacggcgat atcagcgcct ggctgccgct
    15001 cgctagcgcg ttgaacaggc gcgttttcgg cctgcaagca aaatcgccgc agcgctttgc
    15061 cacgctcgac cagatgatcg atgagtatgt cgggtgcatc cgtcgtcagc agcctcacgg
    15121 cccttatgtg ctggcgggtt ggtcgtatgg cgcgtttctt gcggcgggcg ccgcacaacg
    15181 cctgtacgcc aaaggcgagc aggttcggat ggtgttaatc gatcccgtgt gccgacagga
    15241 tttctgttgc gaaaaccggg cggccctgct gcgcctgtta gccgaaggac aaacgcctct
    15301 ggcactgccc gaacatttcg accagcagac gcccgacagc cagcttgccg actttatcag
    15361 cctcgctaaa acggccggta tggtgtcgca aaacctgacg ctgcaagcgg cagaaacgtg
    15421 gctcgacaac atcgcgcatc tgctgcgttt actgactgag catacgccgg gcgaaagcgt
    15481 tccggtcccc tgtctcatgg tgtatgccgc cgggagaccc gcgcgctgga cgccagcaga
    15541 aaccgagtgg cagggctgga taaacaacgc cgacgacgct gtgattgaag ccagccactg
    15601 gcaaatcatg atggaagccc cccacgttca ggcttgtgcg caacacatta cgcgctggct
    15661 ttgcgcaacc tcaacgcaac cggagaacac gttatgatgc cgtccgcctc cccaaaacaa
    15721 cgcatactga ttgtgggcgc caaatttggc gaaatgtacc tgaatgcctt tatgcagccc
    15781 ccggaggggc tggaactggt cggcctgctg gcgcagggaa gcgcccgttc aagagagctg
    15841 gctcatgcgt ttggcattcc gctgtatacc tcgccggaac agataaccag gatgccggat
    15901 atcgcctgta ttgtcgtgcg ttcgaccgtc gccggaggaa ccggtacgca gcttgccaga
    15961 cactttctga cgcgcggcgt acacgtcatt caggagcatc ccctccaccc ggatgacatc
    16021 agttccttac aaacgctggc gcaggaacag ggctgctgct attgggtaaa cacgttttat
    16081 ccgcatacgc gcgcgggacg cacatggctt cgcgatgcgc agcagctgcg tcgctgcctg
    16141 gctaagacgc cgccggtagt ccacgccacc accagccgac agttgctcta ctccactctg
    16201 gatttgttgt tgctggctct gggcgttgat gccgccgccg tggagtgcga cgtggttggc
    16261 agctttagcg attttcactg tctgagactc ttctggccgg agggagaagc ctgcctgctg
    16321 cttcagcgct atctcgatcc tgacgatcca gatatgcata gccttatcat gcaccgcctg
    16381 ctgctgggct ggccggaagg tcatctttcg ctggaggcga gctacggccc cgtcatctgg
    16441 tcatccagcc tgtttgtcgc agaccatcag gagaacgccc acagcctcta ccgcagaccg
    16501 gaaatcctgc gcgatctgcc gggtctgacg cgcagcgcgg cgcccctgag ctggcgcgac
    16561 tgctgcgaaa ccgtcgggcc ggagggcgtc agctggttgc tacaccagtt gcgcagcaat
    16621 ctggcgggcg aacatccacc agcggcctgc caaagcgtac atcagatcgc gttatcacgt
    16681 ttatggcagc agattttacg taagaccggc aatgcggaga tcagacgcct gacgccgccg
    16741 caccacgacc ggttagccgg tttctacaac gatgatgata aggaggcgct gtgacgcaat
    16801 ctgcaatgtg catcccgctg tggcccgccc ggaacggcaa tactgcgcat ctggtcatgt
    16861 gccctttcgc tggcggcagc agtagcgcgt ttcgccactg gcaagctgag caactggctg
    16921 attgcgcgct ttctctggtg acctggccgg ggcgcgatcg ccttcgccat ctggaaccgc
    16981 tcagaagcat tacacaactg gcggcactgc tggcgaacga gctggaagca tccgtatcgc
    17041 ctgacacgcc gcttttactc gccgggcaca gcatgggggc gcaggtggcg tttgaaacct
    17101 gccgacttct ggagcaacgg gggcttgcgc cacaaggact gattatttcc gggtgccatg
    17161 ccccgcatct gcattctgaa cgccagctca gccatcgcga tgatgccgac tttatcgctg
    17221 agctgataga cattggcgga tgttctcctg aactgcggga aaaccaggaa ttaatgtcgc
    17281 tgtttcttcc tcttctgcgc gctgattttt acgccaccga gagctatcac tacgactcgc
    17341 ccgacgtctg tccgccgctg cgcacgcctg cgctgttatt gtgcggcagc cacgatcgcg
    17401 aagcctcctg gcagcaggtc gatgcctggc gtcagtggct gagccacgtt acaggcccgg
    17461 tggtgattga cggcgatcat ttctatccca ttcaacaagc ccggtccttt tttacgcaga
    17521 ttgtccgcca ttttccccac gcattttctg caatgaccgc gtggcaaaaa cagcccagta
    17581 cttcagaaag gtgacgcatg aattcttcct ttgaatctct gattgaacag tatcccttac
    17641 ccattgccga acagttgcgc cactgggcgg cccgttatgc ctcgcgaatt gccgtcgttg
    17701 atgcaaaggg gtcgttaacc tacagcgcgc ttgatgcaca agttgacgaa cttgccgcag
    17761 gtctgtcatc actgggtttg cgttcggggg agcatgtaat tgtgcagctt cccaacgaca
    17821 acgcgtttgt taccctgctg ttcgccttgt taagactggg cgttatcccc gtgctggcga
    17881 tgccctcgca acgggcgctg gatatcgacg cgctgattga gctggcgcaa cccgtcgctt
    17941 acgttattca cggggaaaac cacgcagagc tggcccgaca gatggcgcac aaacacgcct
    18001 gcttgcgtca tgttctggtc gctggagaga ccgtaagcga cgattttacg ccgctcttct
    18061 cccttcacgg tgagcgacag gcatggccgc agcctgatgt ttccgccacc gcgttgttgt
    18121 tgctctcagg cggcacaacc ggcacgccca aactcatccc gcgccgacat gccgactata
    18181 gctataactt cagcgcttct gctgaactgt gcggcatcag ccaacagagc gtgtatctcg
    18241 ccgtcctccc ggtggcgcat aactttccgc tggcctgccc cggtattctg ggaacgcttg
    18301 cctgcggcgg aaaagtggtg ctgaccgaca gcgccagctg tgatgaggtg atgcctttaa
    18361 tcgcgcagga aagagtgact cacgtcgccc tggttccggc gctggcgcaa ttatgggtgc
    18421 aggccaggga gtgggaagac agcgaccttt cgtcgctgcg cgtcattcag gcaggcggcg
    18481 cccggctcga cccgacgctt gctgagcagg ttatcgccac ctttgactgt accctgcaac
    18541 aggttttcgg tatggcggaa ggcctgctct gttttacccg gctggacgat ccgcatgcca
    18601 ccattctcca cagccagggg cgcccgttgt cccctctgga tgaaatccgc atcgttgatc
    18661 aagacgagaa cgacgtcgcg ccgggcgaaa ccgggcaatt gttaacgcgc ggcccttata
    18721 ccatttcggg ctattaccgc gcccctgccc acaacgcgca ggcctttacc gcgcaagggt
    18781 tttaccgcac aggcgacaat gtcaggctgg atgaggtggg gaacctgcac gttgagggac
    18841 gcataaaaga gcagatcaac cgcgccggag aaaaaatagc cgcggctgaa gtggaatcgg
    18901 cactgctgcg tttagcggaa gtgcaagatt gcgcggtggt cgccgcgccg gacacgctgc
    18961 ttggcgagcg gatttgcgcg tttatcatcg cgcagcaggt gccaactgac tatcagcagt
    19021 tgcgtcaaca actgacccgt atggggctca gcgcgtggaa aattcctgac caaatcgagt
    19081 ttcttgacca ctggccgctc accgccgtcg gcaagataga caaaaaacgc ctgacggctc
    19141 tcgccgtcga ccgttatcgc cattctgccc aataagcgca aaccgacccg aaacaggttg
    19201 aaataaaccc gtttcgggta gcactactat tagaaatagt tatcattttc aattcaccat
    19261 tgtcggtatt tttggcgttt cgccgtctta cagggactca caacaatgaa aatgacacgg
    19321 ctttatcctc tggccttggg gggattattg ctccccgcca ttgctaatgc ccagacttca
    19381 cagcaagacg aaagcacgct ggtggttacc gccagtaaac aatcttcccg ctcggcatca
    19441 gccaacaacg tctcgtctac tgttgtcagc gcgccggaat taagcgacgc cggcgtcacc
    19501 gccagcgaca aactccccag agtcttgccc gggctcaata ttgaaaatag cggcaacatg
    19561 cttttttcga cgatctcgct acgcggcgtc tcttcagcgc aggacttcta taatcccgcc
    19621 gtcaccctgt atgtcgatgg cgtccctcag ctttccacca acaccatcca ggcgcttacc
    19681 gatgtgcaaa gcgtggagtt gctgcgaggc ccacagggaa cgttatatgg caaaagcgct
    19741 cagggcggga tcatcaacat cgtcacccag cagccggaca gcacgccgcg cggctatatt
    19801 gaaggcggcg tcagtagccg cgacagttat cgaagtaagt tcaacctgag cggccccatt
    19861 caggatggcc tgctgtacgg cagcgtcacc ctgttacgcc aggttgatga cggcgacatg
    19921 attaaccccg cgacgggaag cgatgactta ggcggcaccc gcgccagcat agggaatgtg
    19981 aaactgcgtc tggcgccgga cgatcagccc tgggaaatgg gctttgccgc ctcacgcgaa
    20041 tgtacccgcg ccacccagga tgcctatgtg ggatggaatg atattaaggg ccgtaagctg
    20101 tcgatcagcg atggttcacc agacccgtac atgcggcgct gcactgacag ccaaaccctg
    20161 agtgggaaat acaccaccga tgactgggtt ttcaacctga tcagcgcctg gcagcagcag
    20221 cattattcgc gcaccttccc ttccggttcg ttaatcgtca atatgcctca gcgctggaat
    20281 caggatgtgc aggagctgcg cgccgcaacc ctgggcgatg cgcgtaccgt tgatatggtg
    20341 tttgggctgt accggcagaa cacccgcgag aagttaaatt cagcctacga catgccgaca
    20401 atgccttatt taagcagtac cggctatacc accgctgaaa cgctggccgc atacagtgac
    20461 ctgacctggc atttaaccga tcgttttgat atcggcggcg gcgtgcgctt ctcgcatgat
    20521 aaatccagta cacaatatca cggcagcatg ctcggcaacc cgtttggcga ccagggtaag
    20581 aacaatgacg atcaggtgct cgggcagcta tccgcaggct atatgctgac cgatgactgg
    20641 agagtgtata cccgtgtagc ccagggatat aaaccttccg ggtacaacat cgtgcctact
    20701 gcgggtcttg atgccaaacc gttcgtcgcc gagaaatcca tcaactatga acttggcacc
    20761 cgctacgaaa ccgctgacgt cacgctgcaa gccgcgacgt tttataccca caccaaagac
    20821 atgcagcttt actctggccc ggtcgggatg cagacattaa gcaatgcggg taaagccgac
    20881 gccaccggcg ttgagcttga agcgaagtgg cggtttgcgc caggctggtc atgggatatc
    20941 aatggcaacg tgatccgttc cgaattcacc aatgacagtg agttgtatca cggtaaccgg
    21001 gtgccgttcg taccacgtta tggcgcggga agcagcgtga acggcgtgat tgatacgcgc
    21061 tatggcgcac tgatgccccg actggcggtt aatctggtcg ggccgcatta tttcgatggc
    21121 gacaaccagt tgcggcaagg cacctatgcc accctggaca gcagcctggg ctggcaggcg
    21181 actgaacgga tgaacatttc cgtctatgtc gataacctgt tcgaccgtcg ttaccgtacc
    21241 tatggctaca tgaacggcag cagcgccgtc gcacaggtca atatgggtcg caccgtcggt
    21301 atcaatacgc gaattgattt cttctgatta ttgtaaaagg gataccgaaa aggtatccct
    21361 tttacaccac tagttaaaac cagtaactca gcagagtcgc aaaaaatatt aatccatagt
    21421 gattatttaa acaatgaaat t
//
LOCUS       DADTFG010000085        21199 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.085, whole genome shotgun sequence.
ACCESSION   DADTFG010000085 DADTFG010000000
VERSION     DADTFG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.085"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            147..1157
                     /gene="glpX"
                     /locus_tag="J1138_002875"
     CDS             147..1157
                     /gene="glpX"
                     /locus_tag="J1138_002875"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBA8454645.1"
                     /translation="MRRELAIEFSRATESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            1254..2000
                     /gene="fpr"
                     /locus_tag="J1138_002876"
     CDS             1254..2000
                     /gene="fpr"
                     /locus_tag="J1138_002876"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBA8454646.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(2005..2433)
                     /gene="uspD"
                     /locus_tag="J1138_002877"
     CDS             complement(2005..2433)
                     /gene="uspD"
                     /locus_tag="J1138_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBA8454647.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(2460..2759)
                     /locus_tag="J1138_002878"
     CDS             complement(2460..2759)
                     /locus_tag="J1138_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBA8454648.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(2971..3411)
                     /locus_tag="J1138_002879"
     CDS             complement(2971..3411)
                     /locus_tag="J1138_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA8454649.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            3512..4111
                     /locus_tag="J1138_002880"
     CDS             3512..4111
                     /locus_tag="J1138_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBA8454650.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            4219..4986
                     /gene="tpiA"
                     /locus_tag="J1138_002881"
     CDS             4219..4986
                     /gene="tpiA"
                     /locus_tag="J1138_002881"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBA8454651.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(5041..5796)
                     /gene="cdh"
                     /locus_tag="J1138_002882"
     CDS             complement(5041..5796)
                     /gene="cdh"
                     /locus_tag="J1138_002882"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBA8454652.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(5903..6892)
                     /locus_tag="J1138_002883"
     CDS             complement(5903..6892)
                     /locus_tag="J1138_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8454653.1"
                     /translation="MKKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKDTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(7212..8174)
                     /gene="pfkA"
                     /locus_tag="J1138_002884"
     CDS             complement(7212..8174)
                     /gene="pfkA"
                     /locus_tag="J1138_002884"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBA8454654.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(8355..9257)
                     /gene="fieF"
                     /locus_tag="J1138_002885"
     CDS             complement(8355..9257)
                     /gene="fieF"
                     /locus_tag="J1138_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBA8454655.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(9406..9906)
                     /gene="cpxP"
                     /locus_tag="J1138_002886"
     CDS             complement(9406..9906)
                     /gene="cpxP"
                     /locus_tag="J1138_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBA8454656.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            10056..10754
                     /gene="cpxR"
                     /locus_tag="J1138_002887"
     CDS             10056..10754
                     /gene="cpxR"
                     /locus_tag="J1138_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBA8454657.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            10751..12124
                     /gene="cpxA"
                     /locus_tag="J1138_002888"
     CDS             10751..12124
                     /gene="cpxA"
                     /locus_tag="J1138_002888"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBA8454658.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(12295..12969)
                     /gene="yiiM"
                     /locus_tag="J1138_002889"
     CDS             complement(12295..12969)
                     /gene="yiiM"
                     /locus_tag="J1138_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBA8454659.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNAYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKLEDFS
                     RRLWGK"
     gene            complement(13118..14101)
                     /gene="kdgT"
                     /locus_tag="J1138_002890"
     CDS             complement(13118..14101)
                     /gene="kdgT"
                     /locus_tag="J1138_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBA8454660.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGDLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARVAKIEILGTVK"
     gene            complement(14361..14981)
                     /gene="sodA"
                     /locus_tag="J1138_002891"
     CDS             complement(14361..14981)
                     /gene="sodA"
                     /locus_tag="J1138_002891"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBA8454661.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQSYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            15266..16300
                     /gene="rhaT"
                     /locus_tag="J1138_002892"
     CDS             15266..16300
                     /gene="rhaT"
                     /locus_tag="J1138_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBA8454662.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(16297..17235)
                     /gene="rhaR"
                     /locus_tag="J1138_002893"
     CDS             complement(16297..17235)
                     /gene="rhaR"
                     /locus_tag="J1138_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBA8454663.1"
                     /translation="MVFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVSFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(17219..18055)
                     /gene="rhaS"
                     /locus_tag="J1138_002894"
     CDS             complement(17219..18055)
                     /gene="rhaS"
                     /locus_tag="J1138_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBA8454664.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            18343..19812
                     /gene="rhaB"
                     /locus_tag="J1138_002895"
     CDS             18343..19812
                     /gene="rhaB"
                     /locus_tag="J1138_002895"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBA8454665.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNTTTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            19809..21068
                     /gene="rhaA"
                     /locus_tag="J1138_002896"
     CDS             19809..21068
                     /gene="rhaA"
                     /locus_tag="J1138_002896"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBA8454666.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGLLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQITPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
BASE COUNT         5278 a         5517 c         5350 g         5054 t
ORIGIN      
        1 gcgcgtctta tcaggcctac gagtcggata cacatccggc atcacacaga ctctgaaacc
       61 gtgcgggggc aaccccgcac acatcaataa tcccgccctt cccctgtgct acacttcgcg
      121 ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa ttttcccgcg
      181 ccaccgaatc agcagcgctg gctggctaca aatggttagg acgcggcgat aaaaacaccg
      241 cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac attgacggca
      301 ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt ggtgaaaaag
      361 tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa ggcacgcgca
      421 tgacggcgat gggccaggct aacgcgctgg cggtgctggc agtaggcgat aaaggctgtt
      481 tcctcaacgc gccagatatg tatatggaga agctgatcgt cggaccggga gccaaaggca
      541 ccattgatct gaacctgccg ctggcggata acctgcgcaa tgttgcggcg gcgctcggca
      601 aaccgttgag cgaactgacg gtaacgattc tggcaaaacc gcgccacgat gccgttatcg
      661 cagaaatgca acaactcggc gtacgcgtat ttgctattcc ggatggcgac gttgcggcct
      721 caattctcac ctgtatgccg gacagtgaag tcgatgtact gtacggcatt ggcggcgcgc
      781 cggaaggcgt ggtttctgcg gcggtgatcc gcgcattaga tggtgacatg aacggtcgtc
      841 tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt ggcgagcagg
      901 agctggcacg ctgcaaagcg atgggcatcg aagccggtaa agtattgcgt ttgggcgata
      961 tggcgcgcag cgacaacgtc atcttctctg ccaccggtat taccaaaggc gatctgctgg
     1021 aaggtattag ccgcaaaggc aatatcgcca ccaccgaaac gctgctgatc cgtggtaaat
     1081 cacgcaccat tcgtcgcatt cagtccatcc actatctgga tcgtaaagac ccggaaatgc
     1141 aggtgcacat cctctgattg atttgatcga ttgagccttc cagtccttcg ggactggaat
     1201 ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa aacatggctg
     1261 attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg tttagtctca
     1321 ccgttcacgc tcccgtgctt ccgtttaccg ccgggcaatt caccaaactt ggccttgaaa
     1381 ttgaaggcga acgcgttcag cgcgcctact cctatgttaa ctcgcccgat aatccggatc
     1441 tggagtttta cctggtcacc gtccccgatg gcaaattaag tccacgactg gcggcactga
     1501 aaccgggcga tgaagtgcag gtggttagcg aagccgctgg cttctttgtg ctggatgaag
     1561 taccagattg cgaaacgcta tggatgctgg caaccggtac agcgattggc ccttatttat
     1621 cgattctgca attgggcaaa gatttagaac gcttcaaaaa catggtgctg gttcacgccg
     1681 cacgatatgc cgccgactta agctatctac ctctgatgca ggaactggaa aaacgctacg
     1741 aggggaaact gcgcattcag acggtggtca gtcgggaaac ggctgcgggt tcgctcactg
     1801 ggcgattacc ggcgttaatt gaaagtggtg aactggaaag cgcggttggc ctgccgatga
     1861 ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc gatacacaac
     1921 agttgctgaa ggagacccgg cagatgacga aacatttacg tcgccgaccg ggccatatga
     1981 cagcggagca ttactggtaa gcggttactt gtcgataaac ggcacgatca agagatccgc
     2041 actcaactta ttgatcatcc cgcgatatgc cggcatcaaa cggttgataa atgagtggtg
     2101 atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca gtgtttccgg
     2161 catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt tcgtcagttt
     2221 atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg ttgcagggaa
     2281 gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg tcaggtgagc
     2341 gtcattatgt ctggcgagct ccagggcttt attcaccagt aaggcatctt cttcattccc
     2401 ggaaattgcc acgccaatgt gtttataagc catatttaac tccttctaaa gcaactccat
     2461 caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca tgagaaggtg
     2521 aaggccgcat caagctcaac ttgcccttcc agatccttaa tcttataggc cacgctggcc
     2581 ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat aaaggactct
     2641 gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc gttatcaatc
     2701 accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac atctttcata
     2761 gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg cgccgttaaa
     2821 ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt gcttaaggtg
     2881 aagagttcag aaggattttc cgggcattgt cacttgcatc gtggaaataa caatcccgga
     2941 aaaaaatagc gataagagat atattaaagg ttaattactt gatttattgt cggctttata
     3001 cttcacatcc tgggtatctt taccatattt attttcgcct tgtgtgccaa caaacgcacc
     3061 aagatcgata agcatcatca ccagaatcaa tgtcgggaca aaacgcccca ccgcccattg
     3121 ccagacaccc ggtaaaattg cccagttacc cgccagcagc atccacgcca caatcatcag
     3181 aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacggtta ctgccgctgt
     3241 tggccaaagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta aatttttacc
     3301 cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa tccagaaatc
     3361 acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca tgtaagttcc
     3421 ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat atcgttttaa
     3481 tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc tgttttttct
     3541 cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag atacggcaac
     3601 gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca gccagtttcg
     3661 agaagacttt aacagccaga atcccagcct gccactgaac gaatttcgtg ccatcgacag
     3721 cagtcccgac aaagccaatc tcactcgtgc agcaagtaaa attaacgaga acttgtatgc
     3781 ttctactgcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaataa cctggctacc
     3841 catccagggg ccagagcaaa aagccgcgaa agcgaaagct caggcataca tggcagcggt
     3901 gatccgcaca ctcaccccat taatgaccaa aactcaaagc cagaaaaaac tgcagtcgct
     3961 actaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac tgcgttatgt
     4021 tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta agctggcgct
     4081 atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt gccgatgaat
     4141 ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt tattcgctta
     4201 taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa actgaacggc
     4261 agccgccaca tggttcacga gctggtatct aacctgcgta aagagctggc aggtgttgct
     4321 ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa gcgtgaagct
     4381 gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc cggcgcattc
     4441 accggtgaaa cctctgctgc tatgctgaaa gacatcggcg cacagtacat catcatcggc
     4501 cactctgaac gtcgtactta ccacaaagaa tctgacgaac tgatcgcgaa aaaattcgcg
     4561 gtgctgaaag agcagggact gactccggtt ctgtgcatcg gtgaaaccga agcggaaaac
     4621 gaagcgggca aaactgaaga agtttgtgca cgtcagatcg acgcggtact gaaaactcag
     4681 ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc aatcggtact
     4741 ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcatccg tgaccacatt
     4801 gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg ctctgtaaac
     4861 gcgtctaacg ctgcagaact gtttgctcag ccagacatcg acggcgcgct ggttggcggt
     4921 gcttctctga aagctgacgc tttcgccgtg atcgttaaag ctgcagaagc ggctaaacag
     4981 gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctaa actcctcgcc
     5041 ttaacgcaaa atctcacact gatgatcctg gatttcttcg gctgaagcac ggttcagcgt
     5101 cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc gtaccatcgc
     5161 cagcccgtag cttcccatat gttcccgcgc ctcaggcact tcttccgcca gcatcataaa
     5221 tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtact catgcccacg
     5281 caaaccacct ggcagtggca accagcggct actgatgttc gccagattgt tatccagctg
     5341 ttcgcgcaca tcaggacgaa tacaagaaat atgaatatga aaatggtttt gcgtacgccc
     5401 ggtgcgggag ttgatcgcca gagataccgc gcgatcggga acaggctggc cgtatttttg
     5461 gctcataaaa tcacgcgcct gccaggctaa ccagaaaaaa ttaggcgttg acggatcggt
     5521 caacaatggg ctttcagtac cgttaatacg gtacgttggc attaacagat attgcagtgg
     5581 gccatgacga tcttttaaaa ccacgtatcc ggcgttgggt ttgacttccg cacatggcga
     5641 cgggttttgg ttttcctgct ggttgggcaa acattgctca agaactatct tacgtaacgt
     5701 gtctgactct tcaccggtta atttccagta accaataccg gcagccacaa cggcgataac
     5761 tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt acctcaaaga
     5821 gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc agtggcgaga aaccaccggg
     5881 cttgataatt atcctgggga aatcagcgtt tgctgatctg atcgaacgta ccgccgttag
     5941 caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg gtgaataact
     6001 tcaattttgg aaacgcattt tcgtactttt tcgctacctc agcgtcgcgc ggacggtagt
     6061 agtttttcgc tgcaatttcc tgaccttctg gcgagtagag atatttcagg taggcttccg
     6121 ccacctcttt ggtgtctttt ttctcgacca ctttatcgac caccgacacg gttggctctg
     6181 cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt tcattcgctg
     6241 ccagcagagc ttcattttcc caggcaatca gtacatcgcc aattccgcgc tcgacaaaag
     6301 tgttagtgga tccgcgcgcg ccagaatcca gaacttcgac gtttttatac agtgcccgaa
     6361 caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag ccccaggctg
     6421 ccaggtaatt ccagcgcgcg ccaccagagc ttttcggatt aggcgtgatc accgaaaccc
     6481 ccggtttaat cagatcgttc cagtcatgga tctgcttcgg attaccctta cgtaccagga
     6541 aaacaatggt ggaagtgtac ggtgcggaat tatccggcag acgtttgatc cactctttat
     6601 caatccgccc gcgttccgca attgcgtcca cgtcataggc cagagccagc gtgacaacat
     6661 cagcttcaat accgttgatt accgacgtcg cttgtttgcc tgaaccaccg tgtgactgac
     6721 gaatcaccac gttatcgcca gtttgctgtt tccagtgggc gctgaatgcc ttgttgtact
     6781 gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata tcctttgcca
     6841 taacgctggt tgccgccagc aaaaatgtta accctacgcc ccactttttc atcgcccgac
     6901 tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttgaccaaa cattaaagaa
     6961 taaaaaaaga ttggctataa cttgcgagta tatgttgggg gattaaaaag gcggaagaaa
     7021 tccgcctcat attgctgaca aagtgcgctt tgtccatgcc ggatgcggca caaacgcctt
     7081 atccggccta caaaagtttg caaattcaat agattacaga attcatgtag gcctgataag
     7141 cgaagcgcat caggcctttt tgcttctgtc atcggtttca ggctaaagga atctgccttt
     7201 ttccgaaatc attaatacag ttttttcgcg cagtccagcc agtcgccttt gaacggacgc
     7261 ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt ctggatacct
     7321 acgcaacgac caccgtaacc tgccagcagc agctcgatag cgtaagcgcc catacgggaa
     7381 gccagaatac ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc cagcacagtt
     7441 gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg ccagttcgtc aacatcacac
     7501 atatgttcgg taatcgccac gatcgcgtgt tttttacctt tcgcgatacc cgctttgatt
     7561 tcgtttacca ggtcttcacg gctgaattca acttccggaa ccacaacgaa ttcacagcca
     7621 ccggcaatgg ccgcagccaa cgtcagatcg ccacaataac ggcccatcac ttccaccacg
     7681 gaaatacgct ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc tacaacggtg
     7741 ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt gtcgatagtg
     7801 cccggcaggc cgatgcacgg gaagcccatt tcggtcagac gcattgcacc catgtaggaa
     7861 ccgtcaccgc cgataaccac cagcgcgtcg atcccacgtt ttttcaggtt ttcgatagcc
     7921 acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg cagaaccgag gaacgtacca
     7981 ccacggttga tcatgtcaga aacgctgtaa cggtctagct gtaccatacg gtcttcatac
     8041 agacccagat agccgtcata aatgcccatt acttccagac cttctgtcag cgcagaacga
     8101 acaaccccgc gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt caacacaccg
     8161 attttcttaa tcatgactac ctctgaactt tggaatgcaa aatgaaatct gttgccggaa
     8221 gtcttcttgc acatcgaagt gatccaacga atgtgcaaat agtataacaa tcacttcctg
     8281 ctgaattgat tcaggtcagg ccaaatggcg gtattttata cacaaaatgc gggtctggct
     8341 ctcttttata ctgattatga aagcatagac cgtttaccct ccctgggtac gacggaacag
     8401 gggtcctgat ggataatcac atccgatccc ggaaaacgcc gtaaaatagc ctgctctacc
     8461 tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc caaatgaatc
     8521 tgaataaagc gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc gctaacaccc
     8581 ggccaggaag tcacgatatc aataatttct tgccgttcct catcaggcaa tgcgcgatcc
     8641 agtaatgact gtaccgcctc atatcccatg cgtaacgcgc tatataaaat atagatgccg
     8701 atccccaatg caaacagagc atcggcgcga tgccagccgt accaggacaa ccccagcgcc
     8761 agcagaattg cgccgttcat cataacatca gactggtaat gtagcatatc agcccgcacc
     8821 gcctggcttt gcgtccggcg caccacccaa cgctgaaacg agacaaggat aatcgtacaa
     8881 attagcgcca caattgtcac gataaccccg acgcctggat ctgtcatcgg tgttggagat
     8941 accagatgtt gaatacccgt caaaaacagg aatagtgccg aaccggagat aaacatactt
     9001 tgcgccagcg ccgcgaggga ctcagcttta ccgtgaccaa acgagtgatt atcgtcggca
     9061 ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat atccaccagc
     9121 gaatccacca gcgcggcgag aatactcacc gacccggtat accaccatgc aaaaatttta
     9181 atcagcaata gcagcgaagc catcgccgtc gcagcaatcg ccgcccgact gaccagccgt
     9241 ccataagatt gattcataaa tactcccgct atcaactgac ggtagtataa cggaagcaaa
     9301 tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc cacatcatgt
     9361 gggggaagac agggatggtg tctatggcaa ggaaaacagg gtttactact gggaacgtga
     9421 gttgctacta ctcaatagct tcaacgatga acttttttgc cattgcgtca cgtcgcgcaa
     9481 ctgctccatt cgttgttgat gtttctcgtt taaaaccgct tgctgctccg gcgttaacag
     9541 gcgatacatt tggttgcgga cttttgccat ctcaacctga cgagcaattt gctcattcgc
     9601 cattttttct gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg tgacaaggcg
     9661 atgcattgtc tccagttcgc taacattaac aggaggctgt tcgtgccggg cctgttgcat
     9721 aagatctcgc atctgctgac gctgatgttc ggttaaactt atgccgtcga acatatggct
     9781 ctgcgtactg cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg aaccgacttc
     9841 agcagcgtgg cttaatgaac tgactgccag cgttgaggcc atgacggcag cggtaactat
     9901 gcgcatcatt tgctcccaaa atctttctgt cgcgattcaa cgatagagag tttacgattc
     9961 aggctgcaaa catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct
    10021 gatgacgtaa tttctgcctc ggaggtattt aaacaatgaa taaaatcctg ttagttgatg
    10081 atgaccgaga gctgacttcc ctattaaagg agctgctcga gatggaaggc ttcaacgtga
    10141 ttgttgccca cgatggggaa caggcgcttg atcttctgga cgacagcatt gatttacttt
    10201 tgcttgatgt aatgatgccg aagaaaaatg gtatcgacac attaaaagca cttcgccaga
    10261 cacaccagac gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg
    10321 ggcttgagct gggcgcagat gactatctcc caaaaccgtt taatgatcgt gagctggtgg
    10381 cacgtattcg cgcgatcctg cgccgttcgc actggagcga acaacagcaa aacaacgaca
    10441 acggttcacc gacactggaa gttgatgcct taagtctcaa tcctggtcgc caggaagcca
    10501 gcttcgacgg gcaaacactg gagttaaccg gtactgagtt taccctgctc tatttgctgg
    10561 cacagcatct gggtcaggtg gtttcccgtg agcatttaag ccaggaagtg ctgggcaagc
    10621 gcctgacgcc tttcgaccgc gctatcgata tgcacatttc caacctgcgt cgtaaactgc
    10681 cggatcgtaa agatggtcac ccgtggttta aaaccctgcg tggtcgcggc tatctgatgg
    10741 tttctgcttc atgataggca gcttaaccgc gcgcatcttc gccatcttct ggctgacgct
    10801 ggcgctggtg ttgatgttgg ttttgatgtt acccaagctc gattcacgcc agatgaccga
    10861 gcttctggat agcgaacagc gtcagggtct gatgattgag cagcatgtcg aagcggagct
    10921 ggcgaacgat ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg cgattgataa
    10981 gtgggcaccg ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc
    11041 tgaacgcagc gaaatgcaga tcattcgtaa ctttattggt caggccgata acgccgatca
    11101 tccgcagaag aaaaagtatg gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg
    11161 cgaagataat taccaacttt atctgattcg tccggccagc agttctcaat ccgatttcat
    11221 aaacttactg tttgaccgcc cgctattact gctgattgtc accatgctgg tcagtacgcc
    11281 gctgctgttg tggttggcct ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc
    11341 cgatgaagtt gcccagggaa acttacgcca gcacccggaa ctggaagcgg ggccacagga
    11401 attccttgcc gccggtgcca gttttaacca gatggtcacc gcgctggagc gcatgatgac
    11461 ctctcagcag cgtctacttt ctgatatctc tcacgaactg cgcaccccgc tgacgcgtct
    11521 gcaactgggt acggcgttac tgcgccgtcg tagcggtgaa agcaaggaac tggagcgtat
    11581 tgaaactgaa gcgcaacgtc tggacagcat gatcaacgat ctgttggtga tgtcacgtaa
    11641 tcagcagaaa aatgcgctgg ttagcgagac catcaaagcc aaccagttgt ggagtgaagt
    11701 gctggataac gcggcgttcg aagccgagca aatgggcaag tcgttgacgg ttaacttccc
    11761 acctgggccg tggccgctgt acggcaaccc gaacgccctg gaaagtgcgc tggaaaacat
    11821 tgttcgtaat gctctgcgtt attcccatac gaagattgaa gtgggctttg cggtagataa
    11881 agacggtatc accattacgg tggacgacga tggtcctggc gttagcccgg aagatcgcga
    11941 acagattttc cgtccattct atcggaccga tgaagcacgc gatcgtgaat ctggcggtac
    12001 aggtttgggg ctggcgattg tagaaaccgc cattcagcag catcgtggct gggtgaaggc
    12061 agaagacagc ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt ataagcggtc
    12121 ataacgtcaa cattcaggta aaaaaatgcc tgatgcacta cgcttatcag gcctacaaaa
    12181 cctgttgaat ttatgggttt tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg
    12241 agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc ggggttattt
    12301 tccccacaaa cggcgggaaa aatcttcgag cttgccgctc agtcggcgct tttgcatcgt
    12361 tcgcgtccag cttttcgata accctgcggc ggagagtaaa cggtgatact gatcgtcatc
    12421 aaacggcata tgccatgcga tggcggcagc ctcctgcacg gtcacatcgc tgacacggga
    12481 aaccaactcc agtggcgcat cagcagatac caaccctggt gcaattacgc tatacaacca
    12541 ccccacttta cccgtgtttt gcatcaactg cgctatatcg ctgatatcaa aatggtaatt
    12601 gagtttatag caaggtgagc gtggctggct gacctgaatt aatgcctctc cccagcggaa
    12661 aatatcacca atataagcat tactttccgt cagaccgtcg gttgagagat tttcgccaaa
    12721 cgcaggcgca acaaacagct ccgcctgttc cggaaattcc cgcgcccagt agagataatg
    12781 ctcacgagga taatgacaca gcgctctgtc tggcccgccg tggatctttt tctccgcctg
    12841 ttcgtcacct tccagcccca ggtccgtcag catcaactca ccatcaacct ggattttggc
    12901 aattgcgctg ggtttgcctt cgggataagc ctgaatcttg cctgtgtata catcaaccgg
    12961 atatcgcatc tgttccctct ccacgagaaa ttttcccatt agaccatatt tattttatgt
    13021 caggcgtgct gttttcgcgg taagtatcca ggcagaaacg caattgaaaa ccggacgaac
    13081 gttagttcat ccggctgtgg ggattaagga agcaggttta ttttaccgtg cctaaaattt
    13141 cgattttcgc cactctggct ttgactttac gtgaccagat agaggtaaga attggcacca
    13201 gaatcgaagt cacaatgacc gccgtcgcga ccagcgaagt tgctgccgga gccatcggtt
    13261 taaacgcagg caccatttca gcaatcagca caggtgtcgc taccgcggcc cctgcggaac
    13321 tggaagcagc aattccggca gtgccatcgc caccgccaat caatttatcg gcgataatca
    13381 acggaatacc ggtcacgata attactgcca cacccaacag gatccccagc aaacctgtct
    13441 gggcaattac agtcaaatca atggtattgc ccagtgcgaa ggcaaagaat ggaatcagcg
    13501 tctgcaccgc tttgctgaaa aattctcgta actcagggtc aaggtccccc agggcaaagc
    13561 ccaccaggaa cggcaatact gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg
    13621 cagtgcccag aataatcatc gtcatgagcg gcccggactc caacgacatc aacacaaatg
    13681 ccccagcttc ttcttttgtg ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg
    13741 tcatatccat cgccgccacc agcgccaacg ttgaaagtcc ggcaaaaaat ccaacttcaa
    13801 caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt
    13861 tagttaccac cagcgtaccg gatttacgca gtaccgttcc cgtcgcgctt aattttattg
    13921 acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta atcatcccgt
    13981 tggtgaatga tccaaaatat ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa
    14041 ggaatagcgg gacgagcatc atcccccccg ggattttctc aatcgagcgt tttatctgca
    14101 tttccatcac ctgtcggcat atttttaagg gctgatgcgt cattcctcaa cggagcatca
    14161 gtgttgatgg aaacgaagat agacattcat ttgatcaata aaagtgatca aaaccacacc
    14221 aattgaaacg ctgttttatt tttatcggat cattgttttt attttgaaat tgatcacaaa
    14281 aaaaccaccg ccgttggcga tggttcattt tttaagctgc aatgcggcgt aaacgcctca
    14341 ttgcagcagg cggcaaatga ttattttttc gccgcgaaac gtgccgctgc ttcgtcccag
    14401 ttcaccacgt tccagaactc tttaatgtag tccggacggc ggttctggaa tttcagatag
    14461 taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc agaaatggct
    14521 tcgcccatca gcggagagtc ctggttagca gtagaaacca ccgccagttt atcgcctttt
    14581 tgcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttttc aaattctgct
    14641 ttgaagttat caacggaacc gaaatcacgt tcgatagccg ctttcaggtc gccctgcagg
    14701 gtggtgcctt ttttcagacc tttccagaac agactgtggt tagcgtgacc accagcgtta
    14761 ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat cagctcttca
    14821 accggcaggt tggcaaattc tggcaggttt tccagcgccg cgttggcgtt gttgacgtaa
    14881 ctctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg cggttccagg
    14941 gcatcgtaag catacggcag ggatggcagg gtataactca tattcatctc cagtgttgtc
    15001 gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat taaatgatat
    15061 tgaaaatgat tatcaatgcc gtacttttca taagggtatg gttttgcagg aaaatgccca
    15121 agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcggc agcggcggca
    15181 acaggcgaaa ggttaatcga cagcacgatt tttacactca tctcgtcgga gatgtgacgc
    15241 gacgaaaaat gatgaggata agaagatgag taacgcgatt acgatgggga tattttggca
    15301 tttgatcggc gcggccagtg cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg
    15361 gtcatgggaa accatgtggt cagtcggtgg gattgtttcg tggattattc tgccgtgggc
    15421 catcagcgcc ctgttactac cgaatttctg ggcgtattac agctcgttta gtctctctac
    15481 gctgctgcct gtttttctgt tcggcgctat gtgggggatc ggtaatatca actacggcct
    15541 gaccatgcgt tatctcggca tgtcgatggg aattggcatc gccattggca ttacgttgat
    15601 tgtcggtacg ctgatgacgc caattatcaa cggcaatttc gatgtgctga ttaataccga
    15661 aggcggacgc atgacgttgc tcggcgttct ggtggcgctg attggcgtag ggattgtaac
    15721 tcgcgccggg cagttaaaag agcgcaagat gggcattaaa gccgaagagt tcaatctgaa
    15781 aaaagggctg gtgctggcgg tgatgtgcgg cattttctct gccgggatgt cctttgcgat
    15841 gaacgccgca aaaccgatgc atgaagccgc tgccgcgctg ggcgtcgatc ccctgtatgt
    15901 cgctctgcca agctatgttg tcatcatggg cggcggcgca atcatcaacc tcggcttctg
    15961 cttcattcgt ctggcaaaag tgaaagattt gtcgctaaaa gccgacttct cgctggcaaa
    16021 accgctgatc attcacaatg tgttactctc agcactgggc gggttgatgt ggtatctgca
    16081 attctttttc tatgcctggg gccacgcccg cattccggcg cagtatgact acatcagctg
    16141 gatgctgcat atgagcttct atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga
    16201 gtggaacaat gcaggacgcc gtccggtaac ggtgttgagc ctcggttgtg tggtgattat
    16261 tgtcgccgct aacatcgtcg gcatcggcat ggcgaattaa tctttctgcg aattgagatg
    16321 acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa aatagttact
    16381 atcttcaaag ccacattcgg tcgaaatatc actgattaac aggcggctat gctggaggag
    16441 atattgcgca tgacacactc tgacctgtcg cagatattga ttgatggtca ttccagtctg
    16501 ctgacgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac aaaatttatc
    16561 cagcgcaaag ggacttttca ggctagccgc cagccgggta atcagcttat ccagcaacgt
    16621 ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca gcaacatcac
    16681 caactgcccg aacagcaact cagccatttc gttagcaaac gacacatgct gactactttc
    16741 atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca
    16801 gtgtggttgc cctgcgctgg cgctaaatcc cggaatcgct ccctgccagt caagattcag
    16861 cttcagccgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa cggaagcgta
    16921 ggagtgttta tcgtcagcat gaatataaaa gagatcgcca cgggtaatgc gataagggcg
    16981 atcgttgagt acatgcagac cattaccgcg ccagacaatc accagctcac aaaaatcatg
    17041 tgtatgttcg gcaaagacat cttgcggata acggtcagcc acagcgactg cctgctggtc
    17101 gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg ctacctcggc
    17161 cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagga gattcgcgtt
    17221 attgcagaaa accatcccgt ccctgacgaa tatcacgcgg tgaccagtta aactctcggc
    17281 gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccgca gcgataggcg atgtcagtaa
    17341 cgctggcctc gctgtggcgt agcagatgtc gggctttcat cagtcgcagg cggttcaggt
    17401 aacgctgagg cgtcagcccc gtttgctgct taagctggcg atgcagcgta cgcaatgaaa
    17461 gagaaaattg atccgccaca gcgtcccaat tcacctcatc ggcaaaatgg tcctccagcc
    17521 aggccagaag caagttgaga cgtgatgcgc tgttttccag gttctcctgc aaactgcttt
    17581 tacgcagcaa gagcaataat tgcataaata agatctcgcg actggcggtc gagggtaaat
    17641 cattttcccc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc tgcaatacgc
    17701 tgtggttaac gcgccagtga gacggatact gcccatcctg ctcttgtggc agcaactgat
    17761 tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacattg gtcagacaca
    17821 gattatcggt atgttcatac agatgccgat catgatcgcg tacgaaacag accgtcccac
    17881 cggtgatggt atagggctgc ccattaaata catgaatacc cgtgccatgt tcgacaatca
    17941 caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc cggggttcta
    18001 ttgccacgga cgcgttacca gacggaaaaa aatccacact atgtaatacg gtcatactgg
    18061 cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct taccttaaat
    18121 tttcgacgga aaaccacgta aaaaacgtcg atttttcaag atacagcgtg aattttcaga
    18181 aaatgcggtg agcatcacat caccacaatg aagcaaattg tgaacatcat cacgttcatc
    18241 tttccctggt tgccaatggc ccattttcct gtcagtaacg agaaggtcac gaattcaggc
    18301 gctttttaga ctggtcgtaa tgaaattcag caggatcaca ttatgacctt tcgcaattgt
    18361 gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa
    18421 tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca atggcctgca tagccagaac
    18481 ggttatgtca cctggaatgt ggatagcctg gaaagtgcca ttcgccttgg attaaacaag
    18541 gtgtgcgagg aagggattcg tatcgatagc attgggattg atacctgggg cgtggacttt
    18601 gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc
    18661 accaatggcc taatggcgca ggcacaacaa caactcggca aacgcgatat ttatcaacgt
    18721 agcggcatcc agtttctgcc cttcaatacg atctatcagc tacgtgcact gacagagcag
    18781 caaccagaac ttattccaca cattgctcac gctttgctga taccggatta ctttagttat
    18841 cgcctgaccg gcaagatgaa ctgggaatat accaacacca cgaccacgca actggtcaat
    18901 atcaatagcg acgactggga cgagtcgtta ctggcgtgga gcggtgccaa caaagcctgg
    18961 tttggtcgcc cgacgcatcc gggtaatgtc ataggtcact ggatttgccc gcagggtaat
    19021 gagattccgg tggtcgccgt tgccagccat gataccgcca gcgcggttat cgcctcgccg
    19081 ttaaacggtt cacgcgccgc ttatctctct tctggcacct ggtcattgat gggcttcgaa
    19141 agccagacgc catttaccaa tgacacggcg ctggcagcca atatcaccaa cgaaggcggg
    19201 gcggaaggtc gctatcgggt gctgaaaaat attatgggct tatggctgct tcagcgagtg
    19261 cttcaggagc ggcaaatcaa cgatcttccg gcgcttatcg ccgcaacaca ggcacttccg
    19321 gcctgccgct tcatcatcaa tcccaatgac gatcgcttta ttaatcctga cgagatgtgc
    19381 agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga tcccggaaag tgatgctgaa
    19441 ctggcgcgct gtattttcga cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg
    19501 gcgcagctgc gtggtgaaga tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac
    19561 acgctgctca accagctgtg tgccgatgcc tgcggtattc gggtgatcgc agggcctgtt
    19621 gaagcctcga cgctcggcaa tatcggcatc cagttaatga cgctggatga actcaacaat
    19681 gtggatgatt tccgtcaggt cgtcagcact accgcgaatc tgaccacctt tacccctaat
    19741 cctgacagtg aaattgccca ctatgtggcg cagattcact ctacacgaca gacaaaggag
    19801 ctttgcgcat gaccactcaa ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg
    19861 cagtagggat tgatgtcgag gaggcactgc gccaacttga tcgtttaccc gtttcaatgc
    19921 actgctggca gggcgatgat gtttccggtt ttgaaaaccc ggaaggtttg ctgaccggtg
    19981 ggattcaggc tactggtaat tatccgggca aagcgcgtaa tgccagtgag ctacgtgccg
    20041 atctggaaca ggctatgcgg ctgattccag ggccgaaacg gcttaattta catgccatct
    20101 atctggaatc agacacgcca gtctcgcgcg accagatcac accagagcac ttcaaaaact
    20161 gggttgaatg ggcgaaagcc aatcagctcg gtctggattt taacccctcc tgtttttcgc
    20221 atccgctaag cgccgatggc tttacgcttt cccatgccga cgaccgcatt cgccagttct
    20281 ggattgacca ctgtaaagcc agccgccgtg tttcagccta ttttggtgag caactcggca
    20341 caccgtcggt gatgaacatc tggatcccgg atggcatgaa agatattacc gttgaccgtc
    20401 tcgccccacg ccagcgtttg ctggcagcac tggatgaagt gatcagcgag aagctaaacc
    20461 ctgcgcacca tatcgacgcc gttgagagca aattgtttgg cattggcgcg gagagctaca
    20521 cggttggctc caatgagttt tacctggggt atgccaccag ccgccagacg gcgctgtgcc
    20581 tggacgccgg gcatttccac ccgactgaag tgatttccga caagatttcc gccgccatgc
    20641 tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt tcgctgggac agcgatcacg
    20701 tagtgctgct ggatgatgaa acccaggcga ttgccagtga gattgttcgt cacgatctgt
    20761 ttgaccgggt gcatatcggc ctcgacttct tcgatgcctc tatcaaccgc attgccgcgt
    20821 gggtcattgg tacacgcaat atgaaaaaag ccctgctacg tgcgttactg gaacctaccg
    20881 ctgagctgcg caagctggaa gcggcgggcg attacactgc gcgtctggcg ctactggaag
    20941 agcagaaatc gttgccgtgg caggcggtat gggaaatgta ttgtcagcgt cacgatacgc
    21001 cagcgggtag cgaatggctg gagaacgtgc gggcttatga gaaagaaatt ttgagtcgcc
    21061 gcgggtaaac actgccggat gcggcacaag tgccttatcc ggcctacagg tcggcaatag
    21121 ttgtaggcct gataagacgc gacagcgtcg catcaggcat tgattgccgg atgcggcaca
    21181 agtgccttat ccggcctac
//
LOCUS       DADTFG010000086        20729 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.086, whole genome shotgun sequence.
ACCESSION   DADTFG010000086 DADTFG010000000
VERSION     DADTFG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.086"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            71..727
                     /locus_tag="J1138_002897"
     CDS             71..727
                     /locus_tag="J1138_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBA8454667.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEFNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            751..1413
                     /gene="exoX"
                     /locus_tag="J1138_002898"
     CDS             751..1413
                     /gene="exoX"
                     /locus_tag="J1138_002898"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBA8454668.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKIRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1410..3470)
                     /gene="ptrB"
                     /locus_tag="J1138_002899"
     CDS             complement(1410..3470)
                     /gene="ptrB"
                     /locus_tag="J1138_002899"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBA8454669.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     QSAD"
     gene            complement(3679..4338)
                     /locus_tag="J1138_002900"
     CDS             complement(3679..4338)
                     /locus_tag="J1138_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBA8454670.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(4665..5021)
                     /gene="yebF"
                     /locus_tag="J1138_002901"
     CDS             complement(4665..5021)
                     /gene="yebF"
                     /locus_tag="J1138_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBA8454671.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(5088..5378)
                     /gene="yebG"
                     /locus_tag="J1138_002902"
     CDS             complement(5088..5378)
                     /gene="yebG"
                     /locus_tag="J1138_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBA8454672.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            5512..6690
                     /gene="purT"
                     /locus_tag="J1138_002903"
     CDS             5512..6690
                     /gene="purT"
                     /locus_tag="J1138_002903"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBA8454673.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(6746..7387)
                     /locus_tag="J1138_002904"
     CDS             complement(6746..7387)
                     /locus_tag="J1138_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBA8454674.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(7424..9235)
                     /gene="edd"
                     /locus_tag="J1138_002905"
     CDS             complement(7424..9235)
                     /gene="edd"
                     /locus_tag="J1138_002905"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBA8454675.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(9470..10945)
                     /gene="zwf"
                     /locus_tag="J1138_002906"
     CDS             complement(9470..10945)
                     /gene="zwf"
                     /locus_tag="J1138_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBA8454676.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11283..12152
                     /locus_tag="J1138_002907"
     CDS             11283..12152
                     /locus_tag="J1138_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA8454677.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            12280..13722
                     /gene="pyk"
                     /locus_tag="J1138_002908"
     CDS             12280..13722
                     /gene="pyk"
                     /locus_tag="J1138_002908"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBA8454678.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVLPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13854..14825)
                     /gene="lpxM"
                     /locus_tag="J1138_002909"
                     /gene_synonym="msbB"
     CDS             complement(13854..14825)
                     /gene="lpxM"
                     /locus_tag="J1138_002909"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBA8454679.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(14944..16266)
                     /gene="mepM"
                     /locus_tag="J1138_002910"
     CDS             complement(14944..16266)
                     /gene="mepM"
                     /locus_tag="J1138_002910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBA8454680.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(16282..17214)
                     /gene="znuA"
                     /locus_tag="J1138_002911"
     CDS             complement(16282..17214)
                     /gene="znuA"
                     /locus_tag="J1138_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBA8454681.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            17293..18048
                     /gene="znuC"
                     /locus_tag="J1138_002912"
     CDS             17293..18048
                     /gene="znuC"
                     /locus_tag="J1138_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBA8454682.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            18045..18830
                     /gene="znuB"
                     /locus_tag="J1138_002913"
     CDS             18045..18830
                     /gene="znuB"
                     /locus_tag="J1138_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBA8454683.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(18975..19985)
                     /gene="ruvB"
                     /locus_tag="J1138_002914"
     CDS             complement(18975..19985)
                     /gene="ruvB"
                     /locus_tag="J1138_002914"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBA8454684.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(19994..20605)
                     /gene="ruvA"
                     /locus_tag="J1138_002915"
     CDS             complement(19994..20605)
                     /gene="ruvA"
                     /locus_tag="J1138_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBA8454685.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         4943 a         5395 c         5143 g         5248 t
ORIGIN      
        1 cgcctgctaa attatgagta ttttctaaac cgcccccata atttgcagtc attttgaaaa
       61 ggaagtcatt atgtcgttct ggaaagttgc agcagcgcaa tatgagcctc gtaaaacctc
      121 actcaccgaa caggtagctc atcatctgga gtttgtgagg gccgccgcca ggcaacaatg
      181 tcagctgtta gtttttccat cgctttcttt actggggtgt gattattcgc gacgtgccct
      241 tcctgcccca cccgatctct cgctattaga cccgctttgt tatgccgcaa cgacctggcg
      301 aatgaccatc attgccggcc ttcccgttga atttaacgat cgctttattc gtggaattgc
      361 ggtgttcgca ccgtggcgaa aaacgccagg gatctaccat cagagttatg gtgcatgtct
      421 gggccgtcgt tccagaacca tcacggtggt cgatgaacaa ccgcaaggta tggatatgga
      481 cccgacctgt tcactgttta cgacagggca atgtctggga gaacccgacc tgctggcgtc
      541 ggcccgtcgc ttacaatttt tttcacatca gtactcgatt gctgtgctga tggcaaatgc
      601 ccgtggtaac agtgcactat gggatgaaca tggtcgtctc atcgttcgcg ccgatcgtgg
      661 ttcattattg ttagtcggtc agcgttcatc acagggttgg caaggcgata tcattccatt
      721 acgctaggct ttttcggcct ggagcatgcc atgttgcgca ttatcgatac agaaacctgc
      781 ggtttgcagg gagggatcgt tgagattgcc tctgttgatg tcattgacgg aaaaatcgtc
      841 aatcccatga gccacctggt gcgtcccgat cgtcctatta gtccacaggc gatggcgatt
      901 catcgcatca ccgaagccat ggtcgccgat aaaccgtgga ttgaagatgt gatcccgcac
      961 tattacggta gtgaatggta tgtcgcgcat aacgccagct ttgaccgccg cgtactgcct
     1021 gaaatgcccg gtgagtggat ttgcactatg aaactggccc gtcgtttgtg gccagggatc
     1081 aagtacagca atatggcgct atataaaata cggaagctca atgtacagac gccgccgggc
     1141 ctgcatcatc accgcgcgtt gtatgactgt tatatcaccg ccgcgttgct tatcgatatt
     1201 atgaacacct ctggctggac ggcagaacag atggccgata tcaccggacg tccatcgttg
     1261 atgacgacct tcacctttgg caaataccgt ggcaaagcgg tttccgacgt tgccgaacgc
     1321 gatccgggct atctgcgctg gttgtttaat aacctcgaca gcatgagccc ggagctgcgt
     1381 ttaacactga aacattatct ggaaaatact taatccgctg actgagcggg taatgttccc
     1441 tgcgccagcg cgaccagaaa agcatattcc atcgctacgc cttcgtacga tttaaagcga
     1501 ccagatttac cgccatgacc tgagtccatg tcggtacaga gcaataaaag atggtcatcg
     1561 gttttcagct cgcgcaattt agcgacccat tttgccggtt cccaatattg cacctgagaa
     1621 tcgtgtaaac cggtcgttac cagtaaatgc ggataagcct gtgcggtgac gttgtcatac
     1681 gggctgtagc ttttcatata ctcgtaatat tgcggatcct gcggattccc ccactcttca
     1741 aattcgccgg tggtaagagg aattgattca tccagcattg ttgttacaac atcaacaaac
     1801 ggtacctggg cgataacgcc gtggaacaat tccgggcgtt gattaatcgc aacgcccatc
     1861 aacatacccc ccgcactccc gcccatcgca taacagagcg aaggagagcc atagcccagt
     1921 tttaacaatg catcgcaggc atcaagataa tcattaaacg tatttttctt cttcagaaat
     1981 ttaccgtcct cgtaccattg ttgccccagc tcaccgccgc cacgaacatg gacaatggcg
     2041 tagacaaatc cacgatccaa caaactcaag cggctaaaac tgaaatcggc atcaatactt
     2101 gcgccgtaag aaccgtagcc atacaccagt agcgggttgt gtcctttgcg aaaatgtttg
     2161 cgatgataga ccagcgaaac cggaacttcg acgccatcac gggcgactat ccacaggtgt
     2221 tcactgcggt aattcgccgc atcaaaacca ggaacttccg tttgttttaa tacacgacgc
     2281 tcaccggtat ccatatccag ttcaaacaaa gtatctggtg tggtcatgga cgaataacca
     2341 taacgcaatc gcgcggtttc aggttctgga ttgtaggcaa tccaggtcac ataggccgga
     2401 tcatcaaagg caatgccaat gacttcccgg gtcttgcggt taatttgccg caaactggtt
     2461 aacccgcgct gacgctcttc aaccaccagc cagtcggtaa acagcgtaaa cccttccagc
     2521 ataatgttat cacgtggcgg aattaactct tcccactgtt gctcatcacg catacgggta
     2581 cggtataagc caaagttttt accgtggcgg ttggaacgca gataaaaccg atgctggtag
     2641 tgatcaaggc tgtattcgtg atctttgcgg cgaggcagaa aaacaaacgg ctcggcatcg
     2701 gccatttccg cgtccagcag gcgaacttca ctggtggtgg cgctggccag atgaatgacc
     2761 acataatgct tcgaggtcgt tttatgcagg ctgacgtaat aggtatcgtc tttttcttcg
     2821 taaatcagtt tatcttgcga tgctggcgta ccgatggcgt gacgccagac ctgataaggg
     2881 agcagcgtca ctggatgctt gcgaacatag tagaaagtcc aggagtcatt tgcccagaca
     2941 aagctgggtt caacgttatc cagcagttcc gggtaccagt tacctgtttc cagattacga
     3001 aaacgaatgc cgtactgccg tcgggaaaga aaatcttctg ccagtgccat aatggtgtta
     3061 tctggcgtaa tcgccattcc gcccatcgaa taaaactcac tatgagccgc gcgcttgttg
     3121 gcatcgagca atgtttccca ctcgtcccac tcttcactga acgccgattg acgctggtag
     3181 atagcatatt cacagcctgg ttcataaata tgccgatagc ggtagccatt tttgatgtag
     3241 ggcgcagaaa cttctcgttg cggaatgcgg tcgatgattt cctttaagat gcgatcctgc
     3301 aaggcttgtt gcgaggccat cacccgatga ccgtaactat tttcttgttg cagatagtcc
     3361 aggacttccg gctgagaacg cgtatcgtcc cgcagccagt agtaattatc gatgcgcgta
     3421 tcgccatgaa gcgtcatggc gtggggaatg cgggcggctt ttggtagcat gttattgttc
     3481 tttctggttg aaacatctta taagggtggc aaaactcacc ggggatgcaa gcgaaacagg
     3541 gtagtcattg cttagatgat gacaggtaat ggcgcggata tcgactgtta tgcagacaga
     3601 gaaaatcagc ctgttcaaat ggctgtgcgg ttctggataa cccgaaatag tcaacttcag
     3661 gctatccaga gagcggaatt actccgccaa agtacgtttt tgctgttcga gatcgcgttc
     3721 aatgccttca cgaacatcct gggggatttt cagcgcatca cccagtgcat tcaggtaact
     3781 gcgttccata aaatggtcaa tatcaatagc cgcgcagctc aggaaataga tttccagcgc
     3841 ctcttcttca ttgcggaccc cggtagccag gcgttgtgga tccagcggtt gttcgattgc
     3901 ctgctcaatg agtacacgcc ccttctcttc cacaccggct tctcgcaatt gctggtcgat
     3961 tgccgcacgt tctttggcat caatatgacc atcactttta gcggcaaaga ccagcgcaag
     4021 gatcaaacgt tccgtacgct catccagcgg cgtactttgc gcgccaaact gcggttcatc
     4081 ctgatgcgcc gcgcgaattt tatctttgta tttattccac agcaccgtac ccgctaccgc
     4141 tccgccgcca accagtaacg cgtttgtgcc atatttcgta agaagtttac gtgctgattt
     4201 atttgcgacc agcagcccag ccagtccgcc taatgcgcca ggcactaaaa gtttgcccaa
     4261 tccctgatcc gcagacgagg aggtagaaga acggctttgc ccaagcaggg attgcagttg
     4321 atttaaccag ttagccatat ttgctcctca ataaccattt ttagtgcctg tagcatagcg
     4381 gatgaggatg tcagaaaatg tatatcgaga caaaagatgc gcaaataagc ggggcggtag
     4441 gccgttattc aaagaaattc tcgccaggta aggtgttcac tgtatataag gtgataaggg
     4501 tattttaggt gaaagtagtt tgacgttttg agtggaactg tttgatgctg attgcccggt
     4561 tcgacgctgc tgcgtcttct caggcctacg attatgttgc gttgtagggc ggacaaggca
     4621 cccgcgccgc atccgccaac ggtcaatcga attatcagaa gccattaacg ccgctgatat
     4681 tccgccattc ccgctttgca gtccacgctg acctggtaat gaatatcggc acttttacca
     4741 cgcacggtta gcggtactga ccatttatca tctttcccct gaatgtcctg caaactgacc
     4801 catgccacgg gatcggcctg accgacaatt ttttgatcat cagcccagcg cgccacgcga
     4861 ttttgttggt aatcacgttt tacgctcgcg gcaattccgg cagcatccag accttcacac
     4921 tttgggaaag tgaccgactt gctggtttca ttattggcag cgaaaacaga tgcgcaggca
     4981 gaaaccaaca acagccctaa aaacgcccct ctttttttca tgtttttctc catagcacaa
     5041 tgattcagga gaaagcatgg tacaaattgt caggagcgca agttgcttca ggccgcgtgt
     5101 gaggcatctt cctcttcgga ttcagcaccg acgacctgtg gggacggtaa tttgccggtt
     5161 ttcagaatgg tgctcagcac atctttttgt tctgccagcc atagcgaaag ggcttcacgt
     5221 tgctcatctt ccatttgcac tggagaattt gtcagccagg tgtcaagcag atcggccgta
     5281 tcaagcatct tgtcataagc atcggcttcc tttttgctgg taaacgacat tttctcttcg
     5341 ccctcacgaa tgactacgta tttaacttca accgccattt gcagcctctc ataataactg
     5401 tgattttata cagtatattt cttttcagtt gggaaatcaa catcagcaat aaagacacac
     5461 gcaaacgttt tcgtttatac tgcgcgcgga attaatcagg ggatattcgt tatgacgtta
     5521 ttaggcactg cgctgcgtcc ggcagcaact cgcgtgatgt tattaggctc cggtgaactg
     5581 ggtaaagaag tggcaatcga gtgtcagcgt ctcggcgtag aggtgattgc cgtcgatcgc
     5641 tatgccgacg caccagccat gcatgtcgcg catcgctccc atgtcattaa tatgcttgat
     5701 ggtgatgcat tacgccgtgt ggttgaactg gaaaaaccac attatatcgt gccggagatc
     5761 gaagctattg ccaccgatat gctgatccaa cttgaagagg aaggactgaa tgttgtcccc
     5821 tgcgctcgcg caactaaatt aacgatgaat cgcgagggta tccgtcgcct ggcggcagaa
     5881 gagctgcagc tgcccacttc cacttatcgt tttgccgata gcgaaaacct tttccgcgag
     5941 gcggttgccg ccattggcta cccctgtatt gttaaaccgg tgatgagttc ttccggtaaa
     6001 gggcagacgt ttattcgttc tgcagagcaa cttgctcatg catgggagta cgctcagcaa
     6061 ggtggtcgcg ccggagcggg ccgcgtaatt gttgaaggcg tcgttaagtt tgacttcgaa
     6121 attaccctgc taaccgtcag cgcggtggat ggcgtccatt tctgtgcacc ggtaggtcat
     6181 cgccaggaag atggcgacta tcgtgaatcc tggcaaccac agcaaatgag cccgcttgcc
     6241 cttgaacgtg cgcaggagat tgcccgtaaa gtggtgctgg cactgggcgg ttatgggctg
     6301 tttggtgtcg agctgtttgt ctgtggtgat gaggtgattt tcagtgaagt ctcccctcgc
     6361 ccgcatgaca ccgggatggt gacgctaatt tctcaggatc tctcggagtt tgccctgcat
     6421 gtacgtgcct tcctcggact tcctgttggc gggatccgcc agtatggtcc tgctgcttct
     6481 gccgttatcc tgccgcaact gaccagtcag aatgtcacgt ttgataatgt gcagaatgcc
     6541 gtaggcgcag atttgcagat tcgtttattt ggtaagccgg aaattgatgg cagccgtcgt
     6601 ctgggggtag cactggctac tgcagagagt gttgttgacg ccattgaacg cgcgaagcac
     6661 gccgccggac aggtaaaagt acagggttaa acccggcaaa aaaacgctac aaaaatgccc
     6721 gatcctcgat cgggcatttt gacttttaca gcttagcgcc ttctacagct tcacgcgcca
     6781 gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc agccaggaac
     6841 caccgatgca cagcacgctt ttcagcgcca ggtagtcacg gtagttagcc ggagaaatac
     6901 cacctgtcgg gcagaaacgg acctgagaga acggacccgc gatcgcctgc agggctttca
     6961 caccgccgtt agcttcagcc gggaagaatt tgaactcttt caaaccgtag tccataccca
     7021 gcatcagttc ggaaacagtg ctgatccccg gaatcagagg aatagtccct tcggtagcag
     7081 ctttcagcag cggctcggtc agacccgggc taattgcaaa ctgtgcacct gcttcagtga
     7141 cttctgccag ctgctgtgga ttcagcaccg taccggcacc cacaatcgct tcaggcactt
     7201 ctttggcgat agcacggata gcgtcaactg cgcactcggt acgcagagtc acttccagaa
     7261 cgcgcacccc accagcaacc aacgcttttg ccatcggcac cgcgtgttcc agttttttta
     7321 ccacgataac cggtacaacc gggccggtgg tcaggattga ttctgcactt gttttccagt
     7381 ttttcatcag agttttctct cgcctgatta caaatttgtc gtcttaaaaa gtgatacagg
     7441 ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc gctgaataat tcacgtcctg
     7501 ttcccacgcg tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc agttccgctt
     7561 cgtctaccag cagcgtcagt tcgcctgtct gtccattcac acgaatgatg tccccgtcgc
     7621 gcacttttgc cagcagcccg ccatcgtagg cctctggtgt tacgtggata gctgacggca
     7681 ctttacctga agcgccggag agtcgtccat cggtaactaa cgcaattttg aaacaccggt
     7741 ccaataatac accaagtggc ggcatgagtt tatgtaattc tggcattccg ttcgcttttg
     7801 gcccctgatg acggacaaca acgacacaat cgcggtccag taaacccgct tcaaaggccg
     7861 gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc tggttctcta
     7921 ccggcacggc agaggttttc ataaccgctc gccccaggtt accgcttaac acttttgtcc
     7981 caccatgatg agagaaaggt tgttcgaagg acgcgatcac attgttgtcg agtgattttt
     8041 ctgccccttc ccgccagtcc agttcaccat tattcagcca tggttcaagg gtataacgag
     8101 acagaccaaa gcctgccacc gtattgacat cttcatgcag caggcctgct ttgagcagtt
     8161 cacgtaccag aaccggtacg ccacctgccg cctggaagtg gttaatatcg gccggaccgt
     8221 tcgggtagag acgtgccatc agcggtacaa catcagaaag gtcagagaag tcatcccagt
     8281 taatctgaat accggctgcg cgcgccatcg ccaccaggtg catggtgtgg ttagtggaac
     8341 caccggtcgc cagcagtgca acgataccgt tcaccaccac tttctcatcg atcatcttac
     8401 cgatcggcat ccattcatta ccattaccgg tcatgcgcgt aacctggcgc gcagctgcag
     8461 cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa agaagagcct ggcaactgca
     8521 tccccataaa ctccaccacc atctggttgg tgttggcagt accgtagaaa gtacatgttc
     8581 ccggcgcatg ataagacgcg gcttctgact ccagtagtgc catgcggtcc actttacctt
     8641 cggcataaag ctggcgaata cgcacttttt ctttatttgg caaaccactt gccatcggtc
     8701 cagacggcac aaacaccgca ggcaaatgac caaacgacag ggctgccatg gtcagacctg
     8761 ggacaatctt gtcgcacaca ccgagaaaca gagcaccatc aaacatgtta tgggacagcc
     8821 ccaccgccgc agacatcgca atcacttcgc ggcttaacag cgacaattcc attccatcct
     8881 gcccctgggt gacaccatca cacatcgccg gaacaccgcc cgcaacctga ccaaccgcat
     8941 tcgcttcatg cagggcttta cgaatgattt ccggatagtg ttcataaggc tggtgagcgg
     9001 agagcatgtc gttataggag gtgatgatgg cgatattgtt acgcaacatg cttttcaaag
     9061 aggctttgtc ttctggctgg caggcagcga aaccgtgtgc caggttaccg catgccaact
     9121 gcgaacgatg aacggtcgaa gttttcgctt gttctatccg ggcgaggtaa gcagagcgag
     9181 tctcgcgcga acgttcaatg attcgatttg ttacgcgtaa caattgtgga ttcataaagg
     9241 ctcctgaaat tgagttgtca gagcaggatg attcacaaca cgtttcattc agaggattca
     9301 tgactgaaac gcctgtaacc ggagctcata gggcaaaaac gtttcagtca gtgtaataaa
     9361 aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt ctacaatctg
     9421 tgcaagatca tgttaccggt aaaataacca taaaggataa gcccagatat tactcaaact
     9481 cattccagga acgaccgtca cgggtaatca tcgccaccga ggcaacgggg ccccaggttc
     9541 cggcctgata cggtttcggc gcatcattgt ccatcgccca cgcctcagta atggagtcta
     9601 cccatttcca ggcttcttcc acttcatcac gacgtacaaa cagtgcctga ataccacgca
     9661 tggtttccag cagcagacgt tcataggcat ctgccagatg cgtttgatta aaggtttctg
     9721 aatagctcag atccagcttg gtgatttgca ggttatgttt gtggtcaagg ccaggaactt
     9781 tattcagtac ctggatatcc acgccttcat caggttgcag acggatagtc agtttattct
     9841 gcggcagatc ctgccacgac tctttaaaca gattcagttc aggtgttttg aaatagacca
     9901 cgacttcaga acatttggtc ggcagacgtt taccagtacg caggtagaat ggcacaccgg
     9961 cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta ttgctgctct
    10021 tgttcgcgcc ctcttcttcc agatagcccg gcactttttt gccctgagcg aagcccgcag
    10081 tatattgccc gcgtacggtt ttttcgcgca cgttggagcg gtcgatgcgg cgcagagact
    10141 tcagtacttt cactttttca tcgcggatgc tgtctgcgct caggtcagac ggcggagaca
    10201 tcgcaatcat gcaaagaatt tgcagcaggt ggttctggat catgtcgcgc atctgaccgg
    10261 ctttatcaaa atagccccag cgcccttcga tccccacttc ttctgccacg gtaatctcaa
    10321 catgatcaat ggtgcgattg tcccagttgt tcacaaacag agagttagca aaacgcagcg
    10381 ccaacaggtt cagcactgtt tctttaccaa gatagtggtc gatacggtaa acctggcact
    10441 cctcgaagta ttcgccaacc tgatcgttga tttcctgcga ggtcgccagc gacgtcccca
    10501 gcggtttctc catgactacg cgtgccggtt tagcattcag ttttgcctca ccaagccctt
    10561 tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta gttaatggtg atacgatttt
    10621 tttgatccag catcgcgccg agacggctga atgcagcagt gtcattgaca tcaagattac
    10681 aaaaatccag acgtgcactc agggtgtccc ataaaccttc atcaatggtt tctttcatga
    10741 aagtttcgag cgcctcgcgg acaactttgg tataagccgc tttatcccag tcagcacgcc
    10801 ctacgccgat aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg
    10861 aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aggtcacagg
    10921 cctgggctgt ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa
    10981 gccagggtat acttgtaatt ttcttacggt gcactgtact gcttttacga gcttgcgaaa
    11041 actgtaaacg cttatccacc cgtgcgatta cggtaaaagc gcgcaaagtg cggcaaaact
    11101 gataaaaaaa tcatcgtttt ccattgggta aaaatctgac actgatcatg ttatgaaaaa
    11161 aaataacaac ttttttatct gcttttgtca ttaacacagc acacaggcgt aatatctgac
    11221 aaaactgcat ttcgatttct ttcagagcgg aaatcgtcat tacccgtgag tctctttaca
    11281 tcatgaatat gctggaaaaa atccagtctc agctggaaca tttgagcaaa tctgagcgca
    11341 aagttgccga ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgctc
    11401 tggcgctgga agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca
    11461 cacgcggttt tcctgatttt aaacttcatc tggcacaaag tctggcgaat ggcactccct
    11521 atgttaatcg caacgtcaat gaagatgaca gcgttgaatc atacacgggg aaaatatttg
    11581 agtccgcaat ggcaacgctc gatcatgtcc gtcattcact ggataaatct gccatcaacc
    11641 gcgccgtcga cttgctcact caggcaaaaa aaatcgcctt tttcggatta ggttcttcag
    11701 ccgccgttgc ccacgatgcg atgaataagt tctttcgttt taatgttccg gtggtgtact
    11761 ccgatgatat cgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga gacgtggtgg
    11821 tgctgatttc tcatactgga agaacaaaaa atctggtcga gctggcgcag ctggcacgcg
    11881 aaaacgacgc tatggtaatt gccctcacct ctgcgggtac cccactcgcc cgggaagcaa
    11941 cgctggcaat taccctcgac gtaccggaag atactgacat ttatatgccc atggtttctc
    12001 gacttgcaca gctgaccgtg atagatgtgc tggcgacagg atttactttg cggcgcggta
    12061 caaaattcag agataacttg aagcgggtca aggaagcgct gaaggaatcg cgttttgata
    12121 agcagttact taatttaagt gacgatcgct aaaaacgact gtcactgtcc taatcttata
    12181 cgacatccga atgagattaa tttatcgcca tcacggcgtt atttcattcg gatttcatgt
    12241 tcaagcaaca cctggttgtt tcagtcaacg gagtattaca tgtccagaag gcttcgcaga
    12301 actaaaatcg ttaccacgtt aggcccggca acagatcgcg ataataacct tgaaaaagtt
    12361 atcgcggcgg gtgccaacgt tgtacgtatg aatttttctc acggctcgcc tgaagatcac
    12421 aaaatgcgcg cggataaagt tcgtgagatt gccgcaaaac tggggcgtca tgtggctatt
    12481 ctgggtgacc tccaggggcc caaaatccgt gtatccacct ttaaagaagg caaagttttc
    12541 ctcaatattg gggataaatt cctgctcgac gccaacctgg gtaaaggtga aggcgacaaa
    12601 gaaaaagtcg gtatcgacta caaaggcctg cctgctgacg ttttgcctgg tgacatcctg
    12661 ctgctggacg atggtcgcgt ccagttaaaa gtactggaag tccagggcat gaaagtgttc
    12721 accgaagtga ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa acttggcggc
    12781 ggtttgtcgg ctgaagcgct gaccgaaaaa gacaaagcag acattaagac tgcggcgttg
    12841 attggcgtag attacctggc tgtctccttc ccacgctgcg gcgaagatct gaactatgcc
    12901 cgtcgtctgg cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt tgaacgtgcg
    12961 gaagccgttt gcagccagga ggcaatggat gacatcatcc tcgcctctga cgtggtaatg
    13021 gttgctcgcg gcgacctcgg tgtggaaatt ggcgatccgg aactggtcgg cattcagaaa
    13081 gcgttgatcc gtcgtgcacg tcagctaaac cgcgcggtaa tcacggcgac ccagatgatg
    13141 gaatcaatga ttactaaccc gatgccgacg cgtgcagaag tcatggacgt agcaaacgcc
    13201 gttctggatg gtactgacgc tgtgatgctg tcggcagaaa ctgctgctgg tcaatacccg
    13261 tcagaaactg ttgcagccat ggcgcgcgtt tgcctggggg cggaaaaaat cccgagcatc
    13321 aacgtttcta aacaccgtct ggacgttcag ttcgacaatg tggaagaagc cattgccatg
    13381 tcagcaatgt acgcggctaa ccacctgaaa ggcgttacgg cgatcatcac catgaccgaa
    13441 tcgggtcgta ccgcgctgat gacctcccgt atcagctctg gtctgccaat tttcgccatg
    13501 tcacgacatg aacgtacgct gaacctgact gctctctatc gcggcgttac gccggtgcac
    13561 tttgatagcg ctaatgacgg cgtagcagct gccagcgaag cggttaatct gctgcgtgat
    13621 aaaggttact tgatgtctgg tgacctggtg attgtcaccc agggcgacgt gatgagtacc
    13681 gtgggttcta ctaataccac gcgtatttta acggtagagt aagtacgttg ccggatgcgg
    13741 cgaaaacgcc tcatccggcc tacagttcaa tgataattca acagatttcg aatcttctga
    13801 agcaaacttg aacttatcat cagtcgaagg cctctcctcg cgagaggcat tttttatttg
    13861 atgggataaa gatctttgcg cttatacggt tggatttcgc ccggtttgcg agttttcagc
    13921 aattttaata tccaggtgta ttgttctggt cgcggaccaa caaaaatctc gacttcttca
    13981 ttcatccgcc gcgcaatcgt atgatcatcc gcctctaaca gatcatccat cggtgggcgc
    14041 acctgaatcg tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg tacaacgcgc
    14101 gcacggcaca ctttcatcaa acgaccaatc gcgggcaacg tcgctttata ggtggcaaag
    14161 aaatcaacaa attcgctgtg ttctgggcca tgatcctgat cgggtaaata atatccccag
    14221 taaccctgac gtaccgactg gatgaatggt ttaataccat catttctcgc atgcagacga
    14281 ccaccaaagc gacggcgcac cgtgttccag acataatcaa aaaccgggtt gccctgatta
    14341 tggaacatcg ctgccatttt ctgcccttgc gaggccatca gcatggcagg aatatcgacg
    14401 gcccaaccgt gcggcaccag aaaaatcact ttctcgttat tacgccgcat ctcttcgatg
    14461 atctccagcc cttgccagtc aacgcgcggc tgaattttct ccggcccgcg tattgccaac
    14521 tcagccatca ttaccatcgc ttgcggcgcg gtggcaaaca tctcatctac aatcgcttcg
    14581 cgttcagctt cactacgttc tggaaagcag agcgacagat tgattaacgc acgacggcgt
    14641 gagctttttc ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg atcacggaac
    14701 tttggcggcg ttaaagcgat acccgccatc gctgctacgc ccagccatgc tccccagtag
    14761 cgcgggtggc gaaaggattt atcaaactca ggaatgtatt cgctattatt ttttttcgtt
    14821 tccatgcttt tccagtttcg gataaggcaa aaatcaatct ggtgatagtg tagcggcgca
    14881 acttgccccg caccaaataa aaaagccggt actgactgcg taccggctgc gaatggatgt
    14941 taattaatca aaccgtagct gcggcacaat ctctttggcc tgtgccagga attcgcgacg
    15001 atcggagccg gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg
    15061 ctggtttatc catacttcat agtgcagatg cggcccggtt gaacgtccgg tattaccgga
    15121 aagcgcgata cggtcgccac gtttcacctt ctgtcccggt ttcaccagga tcttgcgcaa
    15181 gtgcatataa cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc
    15241 gccactgcgt ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccttg
    15301 tggcatggca aaatcaacac ctctgtgtgg cgcaacgcga ccggtcaccg gattagtacg
    15361 acgcgggtta aagttagatg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc
    15421 tttcgccaga ccagtaccgt tacgatcgta gaatttgcca tcttcagcgc ggattgcgta
    15481 ataatcttta ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc
    15541 atcaagcatt tctcgtgaca ttaacaccgc aaattcatcg ccttttttca gtttgcggaa
    15601 atccatttgc cactgcatgg ctttaatcac tgcgctcact tcggcgctgg ttaaaccggc
    15661 gtttctggcg ctggcaacaa agcttccccc gacggtacct ttcagcagat tgttgaccca
    15721 ctctccttgc tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt
    15781 tcgggtttca cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa
    15841 tgtccaggag agttgttgac cgattttcag gttacgcaat tctttgtcgg cagcagccag
    15901 ttgggtgata tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc
    15961 agtggaaaca acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc
    16021 ctggggaatg gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa
    16081 gagcgaacga atttcgttct gttccagctc aatggttttg acaattggcg tggcatcgcg
    16141 gtgataaaca tagggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa
    16201 cataacgcgg tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc gggctatctg
    16261 ttgcacgtaa tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa
    16321 ttgattcagg aattctgaat agcttgtttt acccagtttg atattcgtcc ccaggggatc
    16381 caacgttccc atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa
    16441 ctgtggctca gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg
    16501 taaacgctgc gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag
    16561 tccgaactgt ttttcgaagt agccgtaagc atcgtgaaaa acgaaataac ctttcccctt
    16621 gagcggcgcg agctcgttac caacctgcgt ttcggttgag gctaattgtg cctcaaaatc
    16681 cttcaggttg gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg
    16741 gattgcaacc gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc
    16801 gccgtgatgg tgatcttcgt cacttttttc cgcgtgatcg tgatcatcat catcgtcgtg
    16861 aatacttttc atcagcagcg gtttcacatc ttctagctgc gcaatcgtta cctgtttcgc
    16921 ttctggtaat ttacttaccg gtttttgcat gaacgcttcc atctccgggc caacccaaac
    16981 gactaagtcc gcgttctgta agcgttttac atccgatgga cgcagtgaat aatcatgttc
    17041 tgaagcgccg tcaggcagta aaacctgcgt ttctgttacc ccatcagcaa tggcagaagc
    17101 gatgaaccca acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc
    17161 gccccagaga gcggcggata atgctgcgaa aagaagcgtt tttttatgta acataatgcg
    17221 accaatcatc gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag
    17281 actaaaatta acatgacaag tctggtttcc ctggaaaatg tctcggtttc ttttggccaa
    17341 cgccgcgtcc tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt
    17401 gggccaaacg gcgcaggtaa gtcgacactg gtacgggtag tgctcggact ggtaacaccc
    17461 gatgaagggg ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc gcagaagctg
    17521 tatctcgaca ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggcaca
    17581 cataaagaag atattttgcc tgcactgaaa cgtgtccagg ccgggcatct gattaacgca
    17641 ccgatgcaaa agctctcggg tggcgaaacg cagcgtgtac tgttagcgcg agcattgtta
    17701 aatcgaccgc aattattagt gctggatgaa cccactcagg gcgtggatgt gaatggtcag
    17761 gtggcgttat atgaccttat tgaccaactg cgtcgtgaac tggattgtgg cgttttaatg
    17821 gtatctcacg atctgcatct ggtaatggca aaaaccgatg aagtgctttg cctgaatcac
    17881 cacatttgtt gttccggcac accggaagtt gtttccctgc atccggagtt tatttctatg
    17941 tttggtcctc gtggtgctga acaactgggt atctatcgcc atcatcataa tcatcgtcac
    18001 gatttacagg gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat
    18061 ttcccggttg gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg
    18121 tagtctggcg tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggcg
    18181 tcgcgtttgg tttgttgctg gacgtgaatc cattctatgc ggtgattgca gttacgctgc
    18241 tgctggcggg cggtctggta tggctcgaga agcgtccaca gctggcgatc gacacgttat
    18301 tagggattat ggcgcacagt gccctgtcgc tgggcctggt ggtcgttagt ctgatgtcta
    18361 atattcgtgt tgatttgatg gcttacctgt tcggtgattt actggcagtg acgccagaag
    18421 atctcatctc tattgcgatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc
    18481 gcaatttgct gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac
    18541 agcgcgtgaa attgttgttg atgctggtga cggcattgac gattggtgta gcgatgaaat
    18601 tcgtcggcgc gttgattatt acttcactgc tgattattcc tgctgctact gcacgtcgct
    18661 ttgcccgcac gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtga
    18721 ctggcggttt aaccttttcc gcattttacg atacacctgc aggcccgtcg gtggtgctat
    18781 gcgcggcact gttatttatt ctcagtatga tgaaaaaaca ggccagctaa tctttcgctg
    18841 aacatatttg tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggt
    18901 aggcctgata agacgaacag cgtcgcatca ggcacactgc cagtgtcgga tgcggcgcga
    18961 gcgaccaatc cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc
    19021 gtcgtcgcca tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat caaataaggt
    19081 tccagtacat cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga
    19141 cctaccggcc caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa
    19201 tcgaaacctt cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag
    19261 atggtgccat cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca
    19321 atacgtggcg taccgcgcgc acgacgagca acttccagcg cgccgtcatc actcatctca
    19381 agccccataa agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac
    19441 tccagacgtt gcacaatacc aaaacgatcg cgcaacggtg atgtcagcga acctgcgcgc
    19501 gtggttgcac caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga
    19561 ccttcaccaa tcatgatatc cagttggtaa tcttccattg ccggatacaa cacctcttcc
    19621 accactggtg aaagacggtg gatctcatca ataaacagta catcgtgtgg ttcaaggtta
    19681 gtgagcattg ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa
    19741 ttaacgccca tttcattggc gacaatattg gcaagcgtag ttttacccaa ccccggagga
    19801 ccaaaaatca atagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc
    19861 tccatctgcg aacgaacctg cggctgacca acatactctt ccagtaattt agggcggatg
    19921 gcgcgatctg ccacatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca
    19981 atcatccttt acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg
    20041 cgtcagggcg agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagtg
    20101 ccaccagcgc agcaaccgct tcctgttcag catcgtcggt cgccgggctg gcaggagacg
    20161 tgagtaccag gtcggcggct ggcgtaaaga gatcgccatg caaaccttta aatcggtctt
    20221 tcatttcgac aatcaagcgt tcggcggttt ttttgccaat acccggcagt ttcaccagtg
    20281 cccccacttc ttcacgctca acggcattaa cgaactgctg cgctgacatt ccggagagga
    20341 tcgccagcgc caacttcggg ccgacgccgt tggttttgat caactctttg aacaacgtgc
    20401 gctcttgttt attgttaaaa ccatacagca gttgcgcgtc ttcacgcacc acaaagtggg
    20461 tgaaaacgat cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat
    20521 gcacttcata gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga
    20581 tgatgcctct gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct
    20641 gtatgataaa aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt
    20701 ctcgatgatc tgaaaaatgg cgcagtata
//
LOCUS       DADTFG010000087        19968 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.087, whole genome shotgun sequence.
ACCESSION   DADTFG010000087 DADTFG010000000
VERSION     DADTFG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.087"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            161..664
                     /gene="tssB"
                     /locus_tag="J1138_002916"
     CDS             161..664
                     /gene="tssB"
                     /locus_tag="J1138_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBA8454686.1"
                     /translation="MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNL
                     KGKPDNTPLDERTAIAINRYNLNAVISEADIEREFVVPAELSDAPNEQMYVNLKVKSM
                     DDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDTREQLIQE
                     LGIAVQK"
     gene            687..2171
                     /gene="tssC"
                     /locus_tag="J1138_002917"
     CDS             687..2171
                     /gene="tssC"
                     /locus_tag="J1138_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HBA8454687.1"
                     /translation="MSVKEEIAPVSASQTAAPPSLLDEIMAQTRVQPKSESYDITRQG
                     VSAFIAAMLQGDSSAEPINILAVDAMIADIDARTSRQMDAIIHAPEFQELESLLRSLK
                     LLVERADTRENIKVHFLNVTQEELLDDFEFAPEITQSAYYKHVYSSGYGQFGGEPVAA
                     VIGNFAFKNTTPDMKLLKYISQVSAMAHSPFLSSVSSEFFGLDSWTELPGIKEPGAIF
                     EGPAYSRWRALRESEDSRYLGLTAPRFLLRHPYSPDENPVKTFRYHEDVSQSHESYLW
                     GNTSFLLAANLAESFAKYRWCPNIIGPSSGGAVKDLPVHLYESMGQMQAKIPTEVLIT
                     DRREYELAEEGFITLTMRKGSDNACFFSANSVQKPKTFPKTPEGKAAETNYKLGTQLP
                     YLFVISRLAHYIKVIQREQLGSWKERSDLERELNTWIRQYVADQENPPAEVRSHRPLR
                     QAKIEVLDVDGEPGWYQVAISVRPHFKYMGASFDLSLVGRLDKE"
     gene            2176..2601
                     /gene="tssE"
                     /locus_tag="J1138_002918"
     CDS             2176..2601
                     /gene="tssE"
                     /locus_tag="J1138_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBA8454688.1"
                     /translation="MLRRDSGPTGSLFERIREAANPPSYQNPKEALIRSIRRNLRQVL
                     NTRSGSCYGSPELGITDLNDESLASSDFRREIRKSISQCILHYEPRITDVVVTAAAPD
                     EYAPVELRFHIVATIDVSETRGVFEFDILLDNHQRYCVE"
     gene            2607..4448
                     /gene="tssF"
                     /locus_tag="J1138_002919"
     CDS             2607..4448
                     /gene="tssF"
                     /locus_tag="J1138_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBA8454689.1"
                     /translation="MAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVER
                     LMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYLRPVPAMTVIAYEPKTDQLKVPV
                     QVCRNELIRSRSEKSSLTAQKVLPDNHDKIVSSVACHFTLARDIWLQPLRILDTRNAS
                     SLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAG
                     EYRLPLPDLWLEAAGFDNRDALLPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLP
                     DDFPVNAFTLRLHFNRPLPADIKLRQDSLRLHCTPAINLFTHYAEPVRPDGRMAEYPL
                     RASHKHPDAYDIFQVSTVTSKVKVSGTEISPGSQARVWPEFESFQHQMEYSRQREVVY
                     WHHRTKTSLFHYGLEHSIAFVHADGSIPDSSRFNDDVITTSLICTNRMIPARLHTGDI
                     CVAVNKNPAVASFRNVTRPTLPLWPVTDGDMHWSLISAMNLNYLSLLDRETLIQILRT
                     FDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDPFICEGE
                     IYLLGTVLSHFFALYASINSYHCLKIINTESQESWEWQEKMGQHALI"
     gene            4412..5461
                     /gene="tssG"
                     /locus_tag="J1138_002920"
     CDS             4412..5461
                     /gene="tssG"
                     /locus_tag="J1138_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBA8454690.1"
                     /translation="MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQD
                     ISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNGQYVLQTRFLGFSGSQSPLPGYY
                     LDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYAL
                     VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRK
                     VAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRER
                     YLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLGDPASSRLGRTSFI
                     GQPKVPPAITIRIRE"
     gene            5466..6758
                     /gene="tagH"
                     /locus_tag="J1138_002921"
     CDS             5466..6758
                     /gene="tagH"
                     /locus_tag="J1138_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HBA8454691.1"
                     /translation="MEPLMAEEKQQSRQASLTLQVMNGNELESGRAAKCLFSENGGDI
                     GHTPECHWQVQDRAGSIAARACTVIRHDGAYCLRCLTPGLMINLAPASSDALIRLRQG
                     DEIQLGALALKVFLHDGAAVTYDERMATPETIVMNRDSLADTLLTTEGQPAYPGMPFQ
                     HQLAPTVAHGFSSDPLQALQTESLMVADDPIAPRVSRPVAPASDLTGANGINTPFMDL
                     PPGNARHEDGDEFSAMAQYHLAVTPLLRGMECPLTLHNSQDADEFLEEAGRALQAAIK
                     GLLSLQQQQNSLSDKHLRPLEDNPLRLDMDYATALNVMFAEGKSPVHLAAPAAIAESL
                     RNIRHHEEANRAAIVEALRVMLDAFSPGNLMRRFAQYRRSHELRQKMDNAWAWQMYSN
                     YYDELASSRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            6755..7279
                     /gene="tssJ"
                     /locus_tag="J1138_002922"
     CDS             6755..7279
                     /gene="tssJ"
                     /locus_tag="J1138_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBA8454692.1"
                     /translation="MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMEQST
                     ELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFQAADYDQIATTALPDVLGKNYI
                     DHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIEPVESIGHHYRLLV
                     HIRASAIEMKKEEN"
     gene            7282..8628
                     /gene="tssK"
                     /locus_tag="J1138_002923"
     CDS             7282..8628
                     /gene="tssK"
                     /locus_tag="J1138_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HBA8454693.1"
                     /translation="MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLTRRLDAQGDFFW
                     GFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPDQDLLPEPFQPGTLSSKESHNIY
                     LALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSG
                     ADDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRA
                     ADLAGRIGSPEQSGIADVAEFMMLQMLNRYQMQFTHRSQLHTLHPEAFYRDLVGLLGE
                     LMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVPRAQNLPLFFSEGTW
                     IATINDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLH
                     TLTAAPRQLPYHEGYVYFELEKGTPAWQDVIKAGALALHISGSFPDLNLQLWAIRG"
     gene            8633..9397
                     /locus_tag="J1138_002924"
     CDS             8633..9397
                     /locus_tag="J1138_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HBA8454694.1"
                     /translation="MSDMSEASVFPVEPESGTPGRQYRLALRGNSLNPMIDAATPLLG
                     MVMRLSTMNSQAMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAAL
                     GNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGF
                     RGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIKH
                     VLCGGLVVLTVFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9407..12169
                     /gene="tssH"
                     /locus_tag="J1138_002925"
     CDS             9407..12169
                     /gene="tssH"
                     /locus_tag="J1138_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBA8454695.1"
                     /translation="MIQIDLATLVKRLNPFAKQALEMAASECMSQQASEITVAHVLLQ
                     MLAIPRNDVRVIAERTGISAEDLRQALTVESYPGGRSAEGYPSFSPMLIEWLKESWLL
                     ASAQMQHSELRSGVLLLTLLHSPLRYIPPAAARLLTAINRDQLQQDFAAWTKESAESV
                     DLAGGQTPRATETGDTLLARYAKNMTADARNGRLDPVLCRDYEIDLMIDILCRRRKNN
                     PVVVGEAGVGKSALIEGLALRIMAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEKRF
                     KGLMAEVIFSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATTWS
                     EYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAAAT
                     LSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLEREL
                     RIGLRTDTSRMTEVLEQYDETLSALDELEVAWQQQQTLVQEIIALRQQLLGVAEDDVA
                     SLPDADAVEDTPPEAEQDSTDAESADDAGSVQPEETAETVSPVQRLAQLTAELDALHN
                     DQLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGGTIKGQDLAIASL
                     HKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQ
                     EKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDK
                     GEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFKPALLAR
                     MEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGADVIIGPEVTDEIMSRVTRAENGA
                     RMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDALPDDAVTPQT
                     EDETVL"
     gene            12166..12909
                     /gene="tagO"
                     /locus_tag="J1138_002926"
     CDS             12166..12909
                     /gene="tagO"
                     /locus_tag="J1138_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HBA8454696.1"
                     /translation="MSRLCVFRQTGWFIAGLMAGLPASAAPAETSSVAGVAVAVATAT
                     PPDATATLQAMQSCRQETAALERLDCYDRILAPEQAGFGGAALVKARYQGETWARATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGTD
                     NAAGKLTFNIDGLARVIAPLRDACHWAGE"
     gene            12914..14356
                     /gene="tssA"
                     /locus_tag="J1138_002927"
     CDS             12914..14356
                     /gene="tssA"
                     /locus_tag="J1138_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBA8454697.1"
                     /translation="MAMDLRNPDVWLAHLLENLPEDKLSAALDDGNADWEFVDSEIVK
                     LGSLAHSQLDIPELQRRGLMLLASETKDFRLLAHLLRTLQHAGDILLASRMLAQYTEH
                     YWTCAAPQNMAHKKRFAAQVIKRFESAVQDFAGNAATIQRDALLGELAKLAQCWQAHN
                     ASELAKATDDLFSLFQRAFRDVAPEMTSSARSAAIAPQATTGYVPDMSPHTVAAQPPV
                     AATPIPQVTVESHDDKAWRDTLLKVAAILCERQPESPQGYRLRRHALWQTITSTPQAE
                     SDGRTPLAAFPVDMMDDYLARLNNADMALWQQVEKSLLLAPYWLDGHYLSAQTALRLG
                     YKQVADAIRDEVTDFLARLPALINLLFNDRTPFVSEQTKQWLASSGSVNQTVPVVQTD
                     EELQAARACFDENGLEAALRYLENLPEGDPRHQFHRQFFGAQLLEEAGMVQLAQQQYR
                     MLFRTGLHMMLSEWEPSLLKALEQKLTAEQ"
     gene            14436..17903
                     /gene="tssM"
                     /locus_tag="J1138_002928"
     CDS             14436..17903
                     /gene="tssM"
                     /locus_tag="J1138_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HBA8454698.1"
                     /translation="MPRFKVSATWLLTLAWIFLLVWIWWQGPKWTLYEQHWLAPLANR
                     WLATAVWGLIALVWLTWRVMKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLR
                     LRRHLDNRRYLWQLPWYMVIGPAGSGKSTLLREGFPSDIVYTPESIRGVEYHPLITPR
                     VGNQAVIFDVDGVLTTPGGDDLLRRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLT
                     ADKSRRETLVQNLRQQLQEIRQSLHCRLPVYVVLTRLDLLNGFAALFHSLDKKDRDAI
                     LGVTFTRRAHESDGWRSELGAFWQTWVQQVNLALSDLVLAQTGAAPRSAVFSFSRQMQ
                     GTGEIVTALLAALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLAT
                     WPLVETTPYFTRRLFPEVLLAEPNLAGENSVWLNSSRRRLTAFSTCGAALAALMVGSW
                     HHYYNQNWQSGVNVLAQAKAFMDVPLPQGTDEFGNLQLPLLNPVRDATLAYGDYRDHG
                     FLADMGLYQGARVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNIAPPESEEKLAVLRVV
                     RMMEDKSGRNNEAVKQYMARRWSNEFHGQRDIQAQLMVHLDYALEHTDWHAQRQSSDS
                     DAVSRWTPYNKPVINAQHELSKLPIYQRVYQTLRTKALSVLPADLNLRDQVGPTFDNV
                     FVAGNDEKLVIPQFLTRYGLQSYFIKQHDGLVELTALDSWVLNLTQSVAYSEADREEI
                     QRHITEQYLSDYTATWRAGMDNLNVRDYETMPALTDALEQIISGDQPFLRALTALRDN
                     THALTLSGKLDDKAKEAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQL
                     TELHRYLLAIQNSPVSGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGKLA
                     DQAWHVVMVEAVRYMEVDWRDNVVKPFNEQLADNYPFNPHATQDASLDSFERFFKPDG
                     ILDNFYKNNLRLFLENDLTFGDDGRMLIREDIRQQLDTAQKIRNIFFSQQNGLGAQFA
                     VETVSLSGNKRRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESQLTLIGTSGRAP
                     RSIAFSGPWAQFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYRVHVDTEDNPFTGGLFS
                     QFRLPDTLY"
     gene            17914..19271
                     /locus_tag="J1138_002929"
                     /pseudo
     CDS             17914..19271
                     /locus_tag="J1138_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069182.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19289..19768
                     /locus_tag="J1138_002930"
     CDS             19289..19768
                     /locus_tag="J1138_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBA8454699.1"
                     /translation="MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSL
                     SHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGINEPYYK
                     LKLANARLSNIGLTVPHTINDSPGQAEESVSFTYESINWEHSVAGTSAYSLWSERIF"
BASE COUNT         4669 a         5586 c         5532 g         4181 t
ORIGIN      
        1 tcaaaaatat aaatagttct tttaatattt tatttcatga gtagaatgcc gatcacaaca
       61 gaaggaaggt tacattatct aactgccact gaaacggtat tgtcggtatt tgtatgcagt
      121 acgaaaatgc tgtgctcatg gcctgaacgg gaacattttt atgagcaaaa tgaacaacaa
      181 tggcggcagt gtcgcgccga aagaaagaat cagcgttcgg tatacaccaa aagttgatgg
      241 ggtggctgct gatatcgaat tgccgctgaa tttattgatc accggcaatc tgaaagggaa
      301 accagataac acgccgttgg atgagcgaac tgccattgct atcaaccgct ataacctcaa
      361 cgcagtcatt tcagaagctg acattgaacg ggagtttgtt gttccggctg aactcagtga
      421 cgctcccaat gagcaaatgt acgtcaacct gaaagtcaaa tctatggatg acctttctcc
      481 tgaccatatt gccagccagg taccagaaat aaaacgtctg ctcgaattgc gtgaagccct
      541 ggtcgcactt aagtctccac taggcaatat tccggccttc cgcgcacaac ttcaggcgct
      601 actggaaaac gaagacaccc gcgagcagct gatccaggaa ctgggtatcg ccgttcagaa
      661 ataattttca cagaaggata ttcagtatgt cagtaaagga agaaattgct cccgtcagcg
      721 cgtctcaaac cgccgcgccc ccatctctgc ttgatgaaat catggcgcaa acccgcgtcc
      781 agcctaaatc agaaagctac gatatcaccc gccagggcgt gagcgccttt attgccgcca
      841 tgttacaggg cgacagtagc gctgaaccta tcaatatcct ggccgttgac gccatgattg
      901 ctgatattga tgcccgaacc agcaggcaga tggatgccat tattcatgcc ccggagtttc
      961 aggaactgga atccctgctg cgctcgctga agctgctggt tgaacgcgcg gatacacggg
     1021 aaaacatcaa agtccatttc ctgaatgtca ctcaggaaga actgctggat gatttcgaat
     1081 ttgcacctga aatcacgcag tctgcgtatt acaagcatgt atattcaagc ggttacggac
     1141 aatttggcgg tgagccagtg gcggcggtaa tcggcaactt tgcctttaag aacaccacgc
     1201 cggatatgaa actgctgaaa tatatcagcc aggtcagcgc gatggcgcac agcccgttcc
     1261 tgtcgtcagt atcatccgaa tttttcggtc tggattcatg gacagagtta ccggggatca
     1321 aagaaccggg tgccatcttt gaaggcccgg cctactcccg ctggcgcgcc ctgcgtgaat
     1381 cggaagattc ccgttacctg ggcctgaccg ccccccggtt cctgctgcgc cacccttact
     1441 caccggatga aaaccctgtc aaaaccttcc gttaccatga agatgtcagc cagagtcatg
     1501 agtcttatct gtgggggaat acctcgtttt tactggccgc caacctggcg gaaagttttg
     1561 ctaagtatcg ctggtgccca aatattattg gcccttccag cggcggcgca gtcaaagacc
     1621 tcccggtgca tctgtatgaa tccatggggc aaatgcaggc aaaaattccg actgaagtgt
     1681 tgatcactga ccggagagag tatgagctag cagaagaggg ctttatcaca ctgaccatgc
     1741 gtaaagggtc tgataatgcg tgctttttct cggccaactc ggtgcagaaa cccaaaacat
     1801 tccccaaaac ccccgaaggc aaggctgcgg agaccaacta taaactcggc acccagctgc
     1861 catacttgtt tgttatcagc aggttagcgc attacatcaa ggtaattcag cgcgaacagc
     1921 ttggctcatg gaaagaacgt agtgacctgg agcgtgagct gaacacctgg attcgccagt
     1981 atgttgctga ccaggaaaat ccgcctgccg aagtccgcag ccaccggcct ttgcgtcagg
     2041 ctaagatcga ggtactggat gtggatggtg agccgggctg gtaccaggtc gcgatttctg
     2101 tcagaccgca ttttaaatac atgggtgcca gtttcgacct gagcctggtc ggacgactgg
     2161 ataaggaata atccgatgct aagacgggat tcaggaccaa ccggcagtct ttttgaacgt
     2221 atccgtgagg cggcaaatcc gccttcatat cagaatccaa aagaagcact gatacgttcc
     2281 atcagacgta acctgcgaca ggtcctgaat acccgctccg gcagttgtta tggctcaccg
     2341 gagctgggga ttactgattt aaatgatgaa tcgctggcct ccagcgattt cagacgagaa
     2401 atccgtaagt ccattagcca gtgcatttta cattacgaac ctcgtattac cgatgttgtt
     2461 gttacggctg cggcaccgga tgaatacgcc cctgtggaac tgcgttttca tattgtggct
     2521 acgatcgatg tcagcgagac caggggagtg tttgaatttg acattttgct ggacaaccat
     2581 caacgctatt gtgtggagtg atctctgtgg cttttgaaga acgttactac cgcgaagagt
     2641 tggattatct gcgccagctt ggcaaactgt tggcgcagga aaaaccgcat ctggcccatt
     2701 ttctggccga gaaagagggt gatccggatg tggagcgact gatggaagcc tttgccttta
     2761 tgtccggtag cctgcgccag aaacttgaag acgaatttcc cgaatttacc catgggctga
     2821 tccgtatgct gtgggcaaac tatttacgcc cggttccggc catgacggtt attgcctatg
     2881 aacccaaaac cgatcagtta aaagtccctg ttcaggtctg tcgtaatgaa ctgatcagaa
     2941 gccgctcaga aaagtcctcc ctgactgctc agaaggtgct gcctgacaat catgataaaa
     3001 tcgtttcttc cgtagcctgc catttcacgc tggcgcgcga tatctggctg caaccactgc
     3061 gcattctcga tacccgcaac gccagcagcc tgaaagaagg catcatagat atcagtttta
     3121 ccgcagataa caatgtctcc cccgccatgc ttgacctgaa caaaatcacg ttctggctgg
     3181 gtaatgaaga cgattatacc cgacaccagc tttatctgtg gttttgcgaa tgcctgatgg
     3241 atgcggaact gattgccgga gagtaccgcc tgccgctgcc ggatctgtgg ctggaggcgg
     3301 cgggttttga caaccgggat gctctgctgc cgtggccgaa aaatgtccac agcggctacc
     3361 gcgtgcttca ggagtatttc tgctatcccg aagccttctt ctttttccat ctgcgcgatg
     3421 ttgcgccgct accagacgat ttcccggtca atgcgtttac gctgcgcctg cactttaacc
     3481 gcccgttacc tgccgacatc aagctgcgcc aggattcgct gcgtctgcac tgtacgccag
     3541 ccattaacct gtttacacat tatgcggaac ccgtcaggcc ggatgggcgg atggcggaat
     3601 acccgctgcg cgccagccat aagcatccgg acgcctatga catttttcag gtcagcacag
     3661 tcaccagtaa agtcaaagtt tcaggtactg aaatcagccc aggtagccag gcgcgtgtct
     3721 ggcccgaatt tgaaagcttc cagcaccaga tggagtacag ccgccagcgg gaagtcgtct
     3781 actggcatca ccggactaaa acgtccctgt ttcactacgg gcttgaacat agtatcgcct
     3841 ttgtccatgc agacggtagc attccggaca gctcacggtt taacgatgac gtgatcacca
     3901 cgtcattaat ctgtaccaac cgtatgatcc ctgcccggct gcataccggt gatatctgcg
     3961 tggccgtcaa caaaaatccg gcagtggcct catttcgtaa tgtcacgcgc ccgacactgc
     4021 cgctctggcc agtcacggac ggcgacatgc actggtcgct gatttccgcg atgaacctga
     4081 actacctctc cctgctggac agggagacac tgatccagat cctgcggacc ttcgacctgc
     4141 caggtgctca ccatccgcag cgggccagac tgtcacgcca gaaactggat gccatagaaa
     4201 aactcgaaac caaacctgtt gacaggttgt ttaaaggcgt accggttcgc gggctggcga
     4261 ccacgctgtg gatacgtccc gatccgttta tctgcgaagg tgaaatttat cttctgggta
     4321 ccgtgctctc gcacttcttt gccctctacg ccagcatcaa ttcgtaccac tgtctgaaaa
     4381 tcattaacac ggaaagtcag gagtcctggg aatggcagga gaaaatgggc cagcacgccc
     4441 tgatatagcg acaaaaccgc cgttaccggc ggatgtgcgc aactgcaatt tctatgtgct
     4501 gatggaggcg ctgtaccgtc ggcatggcgc gccgggccag gacatttccc tgcgtaccga
     4561 gcctgcccgg gagatcgtgc gtttcagctc tgacgccagt atcagttttc cgggtacgga
     4621 cctgagtgcg ctttcccgta gccagaatgg acagtatgta ctgcaaaccc ggttcctggg
     4681 tttttccggc agccagtcgc cgctaccggg ctattatctg gatcagatgg cgcaggagtc
     4741 cgcacagaac gaagacggcc tgaaagaatt tctcgatcta ttcagccacc gctggacaca
     4801 gtttgcctat catgcctggc gcaaataccg ctactacatc tgctttcgta gcggcggtac
     4861 tgacaccttt tcacagcgca tgtacgcact ggtcgggctg ggtaatcaga gcgtgcgtga
     4921 caggcttgcc attaaccaca gcaaaatgct ggcctacgcc ggaattctgg caacaccagg
     4981 ccgcgcgccg gaggttatct gtaatctggt gtcgcactgt tttgatttgc cggatgtcac
     5041 cattgaacgc tggcaactgc gtaaagtcgc cattgatccg gcacaccaga accggctggg
     5101 tgtacgtaat ccgaaaggaa aaacggctgg acatattccg ggacgctcca tactcggcgt
     5161 taattttacc ctcggcgcac gggtgccgga tcgcagcgga aaattcctgt tgcagattgg
     5221 cggcctgtcg cgggagcgtt atctctcatt tttacccgat ggcgaaaatc acctgccgct
     5281 gaccatgttc ctttccttta ttctgcgcga ccagtttgcc tgggatttac ggctgtgcct
     5341 ggcgccacag caggctaaag ggatgcgcct tggcgatccg gccagctccc gcctcggcag
     5401 aaccagcttt attggtcagc cgaaagtacc gcccgccatc acaatccgaa tcagggaata
     5461 attctatgga accacttatg gctgaggaaa aacagcaatc ccgccaggcc tcactgactt
     5521 tacaggtgat gaatggcaac gaactggaaa gcggacgcgc cgcaaaatgc ctgttcagcg
     5581 aaaacggcgg tgatattggc catacgccgg aatgtcactg gcaggtacag gaccgggcgg
     5641 gaagcattgc tgcgcgtgcc tgtaccgtta tccggcacga cggagcctat tgcctgcggt
     5701 gcctgacgcc gggactgatg attaatctgg ccccggcctc ttccgatgcg ctcatccgcc
     5761 tgcgccaggg ggatgagatc cagctcggcg cactggcgct gaaagtgttt ctgcatgacg
     5821 gtgcagctgt tacctatgac gagcggatgg cgaccccgga aacgattgta atgaaccgcg
     5881 acagtctggc ggacaccctg ctgaccaccg agggacaacc ggcgtatccg ggaatgccgt
     5941 tccagcatca gcttgccccc acggtggcgc acggtttttc cagcgatccg ctccaggcac
     6001 tccagacgga aagcctgatg gtggcagatg atccgatcgc cccacgcgtt tcccggcccg
     6061 tagcccccgc atccgatctg acaggtgcaa acgggatcaa cacgccgttt atggatctgc
     6121 cgcccggcaa tgcccgtcat gaggacggcg atgaattttc tgccatggcg cagtaccacc
     6181 tggccgtaac cccgctgctg cgcggtatgg agtgtccgct gacgctgcac aattctcagg
     6241 atgcggatga gtttctcgaa gaggccggac gggcgttgca ggccgccatt aagggattgc
     6301 tgagtctgca acaacaacag aatagcctgt cggataaaca cctgcgtcca ctggaagaca
     6361 acccgttgcg ccttgatatg gactatgcca ccgcgctgaa cgtcatgttc gccgagggta
     6421 aaagcccggt acatctcgca gcacctgcgg ccatcgccga aagcctgcgc aacatccggc
     6481 atcatgaaga ggccaaccgg gcggcgattg tggaagcgtt gcgtgtcatg ctggacgcct
     6541 tctcgccggg caacctgatg cgccgctttg cgcaataccg ccgcagccat gagctgcgcc
     6601 agaaaatgga caacgcctgg gcatggcaga tgtacagcaa ctattacgat gaactggcct
     6661 ccagccgaca gcagggattt gaaatgctgt tcaatgaagt ttatgcccag gtctatgacc
     6721 gggtgctgcg cgaaaaacag cgggagccgg aagcatgaac ggcaaagcgt ttctggcctg
     6781 cgttctgatg agcgttgtgt taactggctg tgaaacagcg aaaaaaatca gccaggtgat
     6841 ccgcaatccg gatattcagg tcggaaagct gatggagcag tcaaccgaac tgaccgtcac
     6901 gctgctgacc gagccggaca gcaacctgac ggcggacggc gaagccgcgc cggtggatgt
     6961 ccagttggtg tatctgagcg acgactccaa attccaggcc gccgattacg atcagattgc
     7021 caccaccgcg ctgcccgacg tgctggggaa gaactatatc gatcaccagg acttcaacct
     7081 gttgccggac accgtaaaaa cactgccgcc gatcaagctg gatgagaaaa ccggttatat
     7141 cggtgtcatt gcctattttt cagacgacca ggccacagaa tggaaacaaa ttgagccggt
     7201 agaaagtatc ggccaccact atcgcctgct ggtgcatatc cgcgccagtg cgattgagat
     7261 gaaaaaagag gaaaactgac gatggcaaca atgaaaaaca aggtgatctg gcaggaaggg
     7321 ctgtttgccc tgccgcagca ttttcagcaa cagcagcgtc actgcgagta cctgctgacc
     7381 cgtcgtctgg atgctcaggg tgattttttc tggggcttta cggaactctc catcaatacc
     7441 gaactgcttg cccagggcaa aatcatgatt gaccgtgcgg cgggctgtat gcctgacggc
     7501 accgtattca gcattcccga ccaggatctg ctgccggaac ctttccagcc gggaaccctt
     7561 tcatcgaaag agagccacaa tatttatctg gcgctgcctg taatcagcga cgtgatcaat
     7621 gaaatccagg ggttgcacag cgccggtcag ggtacggagc gttaccgcct cacccatacc
     7681 cgcgtccgtg attttcacac tgacgaaggc gacgaacagc cggtcggtct ggggcagttg
     7741 atccccagga tagtcagcgg cgcagatgat ctcagcgcga tggtgaccct ccccctgtgc
     7801 cgtatcctca ataaaaatgc taccggcgca ctggtgctgg acaacacctt tattcccacc
     7861 attcaggccg tccgtgtcag cgggttgctc ggggcattct ccggcgaggt gcagggactg
     7921 cttgccaccc gcgccgctga cctggccggg cgcattggtt cgcctgaaca gagcgggatc
     7981 gccgatgtgg ccgaatttat gatgttgcag atgcttaacc gctatcaaat gcagttcacg
     8041 catcgttccc aactgcacac gctgcatccg gaagccttct accgggatct ggtggggctg
     8101 ctgggagaac tgatgacctt taccgaaggt aatcgcctgc cctgcaccgt ggagccgtat
     8161 aatcaccggg atcttaccgc gaccttcacc agaaccgtga tcccggaact gcggcgcgcg
     8221 ctcaataccg tactggtgcc gcgtgcgcag aatctgccgt tgttcttttc agaggggacc
     8281 tggatcgcca ccatcaacga tcccacattg ttgcagtcct gcaagctggt actggcagtg
     8341 cgcgccagga tgccctacga ccaggtacaa cgccagttca tccagcagtc gaaagtggcc
     8401 gctaccgaca aaatccgcag cgtggtcagc gtgcaggtgc cgggtattcc gctgcacacg
     8461 cttaccgctg cgccacgtca gttgccatac cacgaaggtt atgtttactt cgaactggaa
     8521 aaaggcaccc cggcctggca ggacgtgata aaagcgggcg cactggcgct gcatatctcc
     8581 ggcagcttcc ccgatctgaa cctccagctc tgggctataa ggggataagt gaatgagcga
     8641 tatgagtgaa gcgtcggttt ttcccgtgga gccggagagc ggaacaccag gacgccagta
     8701 ccgtctcgcc cttcgcggca acagcctgaa tccaatgatt gacgccgcca cgccgttgct
     8761 cggcatggtg atgcgtctgt ccactatgaa cagtcaggcc atgccggaac atctgtttgc
     8821 ccaggtagta accgatgtcc aggccgtaga gcaactactc caggaacagg gatacgaacc
     8881 gggcgtcatt gtttcgttcc gttacattct ctgcacgttt attgatgaag cggcgctggg
     8941 caacggctgg tccaacaaaa acgaatggat caaacagtcg ctgctggttc attttcataa
     9001 cgaagcctgg ggcggcgaga aagtgtttat cctgctcgaa cgcctgatcc gggagccggt
     9061 acgttaccag gatctgctgg aatttttata cctctgtttt tcgctcggct ttcgcgggcg
     9121 ctacaaggtt gccattcagc aacaggatga atttgagcgg atctaccagc gcctgcacca
     9181 tgccctgcac aaactgcgcg gtgacgcgcc atttccgcta cttcatcaga agaacaacat
     9241 acagggtggg cgttatcagt taatccgacg actgaccatc aagcatgtac tgtgcggcgg
     9301 tcttgttgtg ctgaccgtat tttatctgtt ttatctgctg cgtctggaca gccagacaca
     9361 ggatattctg catcagctca ataaattact ccgataagga catcttatga tccagattga
     9421 tttagccacg ctcgtaaagc ggcttaaccc ctttgcaaaa caggcgctgg aaatggctgc
     9481 ctcggagtgt atgagccagc aggccagtga gatcaccgtt gcccatgtgc tgttacagat
     9541 gctggcaatc ccgcgcaacg acgtaagggt gattgccgaa cgcacaggca tcagtgcgga
     9601 ggatttacgc caggcgctga ccgtggagag ttatcccggt ggacgttccg ccgaaggcta
     9661 tcccagcttc tccccgatgc tgatcgaatg gctcaaggag tcatggctgc tggcctcagc
     9721 ccagatgcag cacagcgaac tgcgcagcgg cgtcctgctg cttactctgc tgcattcacc
     9781 gctgcgctat attccacccg ctgctgcccg tctgctgacg gccataaacc gcgaccagtt
     9841 acagcaggat ttcgcagcat ggacaaaaga atccgccgaa tccgtggatc tggctggcgg
     9901 gcaaacgccc cgcgcaacgg aaaccggcga caccctgctt gcccgctacg ccaaaaacat
     9961 gaccgcagac gcccgtaacg gcaggcttga cccggtactg tgccgcgact atgaaatcga
    10021 cctgatgatc gacattctct gccgccgccg taaaaacaat ccggtggtag tgggcgaagc
    10081 aggcgtgggt aaaagcgcgc tgattgaagg gctggcgctg cgcatcatgg caggccaggt
    10141 gccggacaag ctgaaaaaca ccgatatcat gacccttgac ctgggcgcat tgcaggccgg
    10201 tgcgtcggtg aaaggtgagt ttgaaaaacg cttcaagggg ctgatggcgg aggtcatttt
    10261 ctccccggtg ccggtcattc tgtttatcga cgaagcacat accctgattg gcgcgggcaa
    10321 ccagcagggc gggctggata tatccaacct gctcaaacca gcgctggcgc gcggcgagct
    10381 gaaaaccatc gccgccacca cctggagcga gtacaaaaaa tatttcgaga aagatgcagc
    10441 tttgtcgcgc cgcttccagt tagtgaaggt cagcgaaccc aacgctgccg aagccaccat
    10501 tattctgcgc ggtttgtcgg cggtctatga acaatctcac ggcgtgctga ttgatgatga
    10561 cgccttgcag gccgctgcga cactgagcga gcgttatctc tccgggcgtc agttgccgga
    10621 caaagccatt gatgtgctgg acaccgcctg cgcccgtgtg gccatcaacc tgtcatcgcc
    10681 gccaaaacaa atctcggcac tgaccaccct gagccaccag caggaggcag aaattcgcca
    10741 gcttgagcgc gagcttcgca tcgggctgcg taccgacacc tcacgaatga ctgaggtgtt
    10801 ggagcagtat gatgaaacgc tgtcggcgct ggacgaactg gaagtcgcct ggcagcagca
    10861 gcagacgctg gtacaggaaa ttatcgcgct gcgccagcag ttactgggcg tggcagaaga
    10921 cgacgttgcg tcgttgccgg acgcagatgc cgtggaggat acgccgccag aggcagaaca
    10981 ggatagtacc gatgccgaat cggctgatga tgcaggcagc gtacagccgg aagagaccgc
    11041 tgaaacagtt tccccggtac aacggctggc acaactcact gccgaactgg acgccctgca
    11101 taacgaccag ttgctggttt ccccgcatgt cgataaaaaa cagattgcag cggtgattgc
    11161 tgaatggacc ggcgtgccgc ttaaccgcct gtcacagaat gagatgtcgg tcatcaccga
    11221 cctgccggta tggctcggcg gcaccatcaa aggccaggac ctggcgattg ccagcctgca
    11281 taaacacctg ctgaccgcac gcgccgacct gcgccgtccg ggacgcccgc ttggtgcgtt
    11341 cctgctggct ggccccagcg gcgtgggtaa aaccgaaacc gtcctgcaac tggcggaact
    11401 gctctatggc ggacgtcaat acctgaccac catcaatatg tctgagttcc aggagaaaca
    11461 caccgtttcg cggctgattg gctctcctcc gggttatgtc ggctacggtg aaggcggcgt
    11521 actgaccgaa gcgattcgcc agaaaccgta ctcggtggtg ctgctcgatg aagtagaaaa
    11581 agcgcacccg gatgtactca acctgttcta ccaggcgttt gacaagggcg agatggcaga
    11641 cggtgaaggc cgcctgattg actgtaaaaa tatcgttttc ttcctgacct ccaaccttgg
    11701 ctaccaggtg attgtcgaac acgccgatga cccggaaacc atgcaggaag ccctctatcc
    11761 ggtgctggcg gacttcttta aacctgctct gctggcgcgc atggaagtgg tgccttacct
    11821 accgctgtcg aaagagacgc tcgctaccat tattgccggg aaactggccc gcctggataa
    11881 cgtgctgcgc agccgctttg gcgcggacgt gattattggg ccggaagtga cggacgaaat
    11941 catgagccgc gtcacccgcg cggaaaacgg cgcgaggatg ctggaatcgg ttatcgacgg
    12001 cgacatgctg ccgccgctct cgctgctgct gttgcagaaa atggcggcca acacggcgat
    12061 tgcccggatt cgcctgtcgg cggcagacgg tgcgttcacg gcagatgtgg aagatgcgct
    12121 gcctgatgat gccgtgacac cgcaaacaga ggatgaaacg gttttatgag caggttgtgc
    12181 gttttccggc agacaggatg gtttatcgcg ggcctgatgg cgggcttacc cgccagtgcc
    12241 gcgcccgcag aaacatcgtc agtggccggt gtggcggtag ctgtcgccac agcgacaccg
    12301 ccggatgcga cagccacgtt acaggccatg cagtcctgcc gccaggagac ggcggcgctg
    12361 gagcggcttg actgttatga ccgcattctt gcgccggaac aggctggctt tggtggtgcc
    12421 gcactggtga aagctcgtta ccagggtgaa acctgggccc gcgcgacaga acaggaaaaa
    12481 cgccgccagg gcaacaccac ggagctgctg gtaacgcaag taccgggtga acgcccgacg
    12541 gtggtgataa ccaccccggc tatcggccat gttccgccac gcccggtgct gatgttcagt
    12601 tgcgtggaca atatcacccg gatgcaggtg gcgctgatgc accctctgga tgtccatgat
    12661 atcgccgtca ccctgaatgc cgacagccgg gcattgcgca gccactggtt tgtgcgcgag
    12721 aacggcaccc tgctggagtc cagccggggg ttatccggta tcgatgaaat taagcaactg
    12781 ttcggcgcaa aaacgctgac ggtggatacc ggcacagaca acgccgccgg aaagctgacc
    12841 tttaacattg acgggctggc acgggttatt gcgccgctac gcgacgcctg ccactgggcg
    12901 ggagaataag aatatggcaa tggacttacg taatcccgat gtatggcttg cacacctgct
    12961 ggaaaacctg ccagaagata agctgtccgc cgcgcttgat gatggcaatg cagactggga
    13021 gtttgtcgac agcgaaatcg tcaagctcgg ttcactggcg cacagccagc ttgatatccc
    13081 ggaacttcag cgccggggcc tgatgcttct ggcctcagaa accaaagatt ttcgcctgct
    13141 ggcccacttg ctgcgtaccc tgcaacatgc cggtgatatt ctgctggcct ccaggatgct
    13201 ggcacaatat acagagcact actggacatg cgccgcaccg cagaacatgg cccacaaaaa
    13261 acgctttgcc gcccaggtga taaaacgctt tgagtctgcc gttcaggact ttgccgggaa
    13321 tgctgcgaca atacagcgcg atgctttgct gggagagctg gcaaaactgg cacagtgctg
    13381 gcaggcgcac aacgcgtctg agctggcaaa ggcgacagac gatttatttt ccctgtttca
    13441 gcgcgcgttt cgtgatgtag cgcctgaaat gacatcatct gcacgaagcg ccgccattgc
    13501 gccgcaggcc acgaccggat atgtaccgga tatgtcaccg cacacggttg ccgctcagcc
    13561 tcctgttgca gccactccaa tcccgcaggt caccgttgaa agccacgacg ataaagcatg
    13621 gcgcgacacc ctgctaaagg tggccgccat tctctgtgag cgccagccgg agtcaccgca
    13681 gggttaccga ctgcgccgcc atgcgctgtg gcaaaccatc accagtaccc cgcaggcaga
    13741 aagcgatggc cggaccccac tggcagcctt ccctgtcgat atgatggatg attacctggc
    13801 gcggctgaac aatgccgaca tggcgctgtg gcagcaggtg gaaaaaagcc tgctgctggc
    13861 cccgtactgg ctggacggtc attacctctc agcacagacc gcgctgcgcc tcggttataa
    13921 acaggtggcg gatgctatcc gtgatgaagt taccgatttt ctggcccgcc tgcccgcact
    13981 gatcaacctg ttgtttaacg accgcactcc atttgtgtcc gaacagacga aacagtggct
    14041 ggcatcatcc ggctcagtca accagaccgt cccggttgta cagaccgatg aagaattaca
    14101 ggcggccagg gcgtgcttcg atgaaaacgg gctggaagcc gcgctgcggt acctggaaaa
    14161 cctgcccgaa ggtgatcccc gccaccagtt tcaccgccag tttttcggcg cgcagttact
    14221 ggaagaggcc ggtatggttc agcttgcaca acagcagtac cggatgctct tcagaacagg
    14281 attacacatg atgctatccg aatgggagcc ttccctgctg aaagcactgg aacaaaaact
    14341 cacggcagaa cagtaaaaga taacaaggag ttactgtgtt caaatttccc acatcacgac
    14401 tgttcagcac gttgaaatct gcgctcaggc cagcgatgcc gcggtttaaa gtttccgcca
    14461 cctggctact gacgctggca tggatttttc tgctggtgtg gatctggtgg cagggtccaa
    14521 aatggacgct ctatgagcag cactggctgg ctccgctggc aaaccgctgg ctggcgaccg
    14581 ccgtctgggg acttatcgct ctggtctggc tcacctggcg ggtgatgaag cgtctgcaaa
    14641 agctggaaaa acagcagaaa cagcagcggg aggaagaaaa agatccgttg accgtggaac
    14701 tccaccgcca gcagcaatat ctggatcact ggctgctgcg cctgcgccgc catctggata
    14761 accgccgtta tctgtggcag ttgccgtggt atatggtcat tggtcctgcg ggtagcggca
    14821 aaagcacgct gctgcgcgag ggctttccgt ctgacattgt ttacacgccg gaaagcatcc
    14881 ggggtgtgga ataccacccg ctgatcacac cgcgagtggg caaccaggcg gtaattttcg
    14941 atgttgacgg cgtactgacc actcccggcg gggatgatct gctccgccgc cgcctgcgcg
    15001 aacactggct gggctggctg atgcaaacgc gcgctcgcca gccgctcaac ggtcttatcc
    15061 tgacgctcga tcttcccgat ctgctgacgg cggataaatc ccgccgtgag acactggtac
    15121 aaaatttgcg ccagcaactt caggagatcc gtcagagcct gcactgccgt ctgcccgttt
    15181 acgtggtgct gacacggctg gatctgctga acggctttgc cgcgctgttc cattcactgg
    15241 ataaaaaaga ccgcgatgcg atcctcggcg tcacatttac ccgccgcgcc catgaaagtg
    15301 acggctggcg cagcgaactg ggggctttct ggcagacgtg ggtacaacag gtgaacctgg
    15361 cgctgtcgga tctggtgctc gcacaaaccg gtgctgctcc ccgcagcgct gtgttcagct
    15421 tctcccgtca gatgcaggga acaggagaaa tcgtcaccgc actgctcgcc gcattgctgg
    15481 acggtgagaa catggatgta atgctgcgtg gcgtctggct cacatcctcg ctacagcgtg
    15541 gccaggtgga tgatattttc acgcagtccg ccgcccgcca gtacggactg ggtaacagct
    15601 cgctggcaac ctggcctctg gtggagacga cgccgtattt tactcgccgc ctcttcccgg
    15661 aagtcctgct ggctgagccg aacctggcgg gtgaaaacag cgtctggctg aacagctccc
    15721 ggcgcaggct gaccgccttt tccacctgtg gcgcggcact ggcggcattg atggtcggaa
    15781 gctggcacca ttattacaat cagaactggc agtctggcgt taacgtactg gcacaagcta
    15841 aagcctttat ggacgtacca ctaccgcagg gaacggatga attcggcaat ctgcaattgc
    15901 cattgcttaa cccggtacgc gatgccaccc tggcctatgg tgattatcgc gatcacggtt
    15961 ttctggcgga tatgggattg taccagggcg cccgcgtagg gccgtatgtg gagcaaacct
    16021 acattcagct tcttgagcag cgttatctcc cctcgttaat gaacggcctg atccgggatc
    16081 taaacattgc cccgccagag agcgaagaaa agctcgctgt gctgcgcgta gtgcgcatga
    16141 tggaagacaa aagtgggcgc aacaacgagg cggtaaaaca gtacatggcg cggcgctgga
    16201 gcaatgaatt tcacggccag cgcgatattc aggcgcaact gatggtgcat ctggactatg
    16261 cgctggagca caccgactgg cacgcgcagc gccaaagcag cgacagcgat gctgtaagcc
    16321 gctggacccc ctataataaa ccggtcatta atgcgcaaca tgaactgagc aagctaccca
    16381 tataccagcg tgtctaccag acccttcgca ccaaagcatt aagcgtgtta cccgccgatt
    16441 tgaatttgcg cgaccaggtt ggtcccacct tcgacaacgt gttcgtcgcc ggtaatgatg
    16501 aaaaactggt gatcccgcag tttctcaccc gctatggcct gcaaagctat tttatcaaac
    16561 aacacgacgg tctcgttgag ttgaccgcgc tggattcgtg ggtgctgaac ctgacgcaaa
    16621 gcgtcgccta cagcgaggcc gaccgtgaag agatccagcg ccatatcact gaacagtacc
    16681 tcagtgacta taccgctaca tggcgtgccg gaatggataa tctcaacgtc cgtgactatg
    16741 agaccatgcc ggcgctgacc gacgcgctgg agcagattat cagcggtgat cagccattcc
    16801 tgcgtgctct gacggcgctg cgcgataata cacacgcgct gacgctctcc ggcaaactgg
    16861 acgataaggc gaaggaagcg gcgataaatg agatggatta ccgcctgtta tcccggctgg
    16921 ggcatgagtt cgcgccggaa aatagcgcac tggaggagca aaaggacaag gcgagtactc
    16981 tacaggccgt gtatcagcaa ctgaccgagc tgcaccgtta cctgctggcg atccagaact
    17041 cgccagtgtc tgggaaatcg gcgctgaaag cagtacagct acggctggat caaaacagca
    17101 gcgatccaat cttcgccacc cgtcagatgg caaaaaccct gcctgcgcct cttaaccgct
    17161 gggtaggtaa gctggcggat caggcctggc atgtagtgat ggtggaagcc gttcgttaca
    17221 tggaagtgga ctggcgcgac aatgtagtga aacccttcaa cgagcagctt gccgataact
    17281 atccgtttaa tccgcacgcc acacaggatg cctcactgga ttcgtttgaa cgtttcttta
    17341 aaccggatgg cattctggac aatttctaca agaacaacct gcgcctgttc cttgaaaacg
    17401 atctgacctt tggcgacgac ggcagaatgt taatccgtga agatatccgg cagcaactgg
    17461 atactgcgca gaaaatccgc aacatcttct tcagccagca gaacgggctg ggcgcacagt
    17521 ttgccgtgga aaccgtatcg ctttccggca ataagcggcg cagcgtactt aacctggacg
    17581 gccagttagt ggactacagc cagggacgca actacaccgc ccatctggtc tggccgaaca
    17641 acatgcgtga aggcaatgaa agccagctga cgctgattgg caccagcggc agagcaccgc
    17701 gcagtatcgc gttcagcgga ccgtgggcgc agttccgcct gttcggcgcg ggccagttga
    17761 ccaatgtgac cagtgacacc tttaacgtgc gctttaacgt ggacggcggc gcgatggttt
    17821 accgggtgca tgtggatacc gaagataacc cgttcaccgg cggtctgttc agccagttcc
    17881 gtttaccgga tacgttgtat taaggggata gtgatgagca ataacgcact gacacaaact
    17941 atcgttaccg gcagcgatcc gcgcgccctg ccggagttca gcgccatccg cgaagaaatt
    18001 aacaaagcta accacccgtc acagccggag ctgaactgga aactggtgga atcgctggcg
    18061 ctgtcgattt ttaaggccaa cggggtggat ttgcataccg ccacctacta tacgctggca
    18121 cgtacacgta atcagggact ggcagggttc tgtgaaggcg cggaactgct ggctgcgatg
    18181 atcatccacg aatgggataa attctggccg cagagtggcc cggcgcggac tgagatgctg
    18241 gactggttca acacccgcac cgggaatatc ctgcgccagc aggtttcctt ttccgaaaat
    18301 gatctttcgt tgctctaccg cacggaacgg gctttgcagc ttatctgcga caaactccag
    18361 caagtggaac tgaagcgcca gccgcgcgtg gaaaatctgc tttattttgt gcagaacaca
    18421 cgcaagcggt ttgagccgca gcccagaaac cgtactgata cggcggcaca gaccatggtc
    18481 agaacgctgg tctatgcgcc tgaaggcacg gcttccgcca cagcggaaac catgcccccc
    18541 tacccgacct gccggaaatg cgggtggggg tgcgcggtgt ggcagataac gccgacaaag
    18601 caaaacaggg cgacacggta aaaggttttg ttgccggtgc cgcctgtact gccgttatcg
    18661 catctgcact ctggtggtgg caggcgtatc cgatgcagca gcagcttaca caggtcaggg
    18721 ataccacaca gggagccgcc accgtctggt tggcctcccc ggtactgaaa gaatatgaac
    18781 agtacctgca acagcttctg aatgccccgc cactacagcc gctggaaacc ggaatgcaga
    18841 tgatgcgcac cgcagacacc ctctggccgg aaagcctgca acagcaggaa gccagcagga
    18901 tgtggagcaa cacgctgaga aaccgggcgc aagccagtcc gcagatgaag ggctggcaac
    18961 aagcgcggca gaatttgcgg gattttgcag atttgatgat gaagaaggag acggagaaac
    19021 aaggtttcac cctatcgtat atcaaaacgg tgacctggca ggccgaacgg ttactgaatc
    19081 aagaaacacc actggagtac ctgctgacgc agtaccagga gacccgtaca cagaagcagg
    19141 acacacaggc gttggaaaaa gagatcaacg aacggctgga cgggctgtta agccgctggc
    19201 tgctgctgaa aaataccgga caggatatgg caacggataa cagaaccgaa cctgtccatc
    19261 caactcattg agattaagga gaataacaat ggcaaatccc gtctatctga cactgaacgg
    19321 agaacttcag gggctgattt catccggatg ctcgtcaatg ccgtccatcg gtaacaaagc
    19381 ccagattgca catcaggatc agatcatggt gacgtcactg tcccatggtc tgagccgtgc
    19441 gcagaatgtc aaccatcagg agttgaccat cacgaaaccg gtggacaaat cctcccctct
    19501 gctgggcaag gccatttcag agaatgaatg tctgacctgt gattttgtat tctaccgcac
    19561 caaccgcttt ggaattaatg aaccgtacta caagctgaaa ctggctaacg cccggctctc
    19621 aaacatcggc cttaccgttc cccataccat taacgacagt cctgggcagg cggaagagtc
    19681 tgtgtcattt acctatgaga gcatcaattg ggagcacagc gtcgccggaa ccagtgcata
    19741 cagcctttgg agtgaacgga ttttctaagt taccagggag tctttcatct gcattattgt
    19801 tatattttaa aaaggcaatt taacattata ggataaaatg acaaagtacg gttaatcacg
    19861 aataaaaatt cttaattaac cgtacctgct gttttataga tgtatatgat gtatttttga
    19921 cagttcaacc gaaaactcga attacagctt atatgttgag aggacaat
//
LOCUS       DADTFG010000088        19917 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.088, whole genome shotgun sequence.
ACCESSION   DADTFG010000088 DADTFG010000000
VERSION     DADTFG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.088"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            116..541
                     /gene="nudI"
                     /locus_tag="J1138_002931"
     CDS             116..541
                     /gene="nudI"
                     /locus_tag="J1138_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBA8454700.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(580..1182)
                     /locus_tag="J1138_002932"
     CDS             complement(580..1182)
                     /locus_tag="J1138_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBA8454701.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1490..2629
                     /gene="arnB"
                     /locus_tag="J1138_002933"
     CDS             1490..2629
                     /gene="arnB"
                     /locus_tag="J1138_002933"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBA8454702.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2633..3601
                     /gene="arnC"
                     /locus_tag="J1138_002934"
     CDS             2633..3601
                     /gene="arnC"
                     /locus_tag="J1138_002934"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBA8454703.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3601..5583
                     /locus_tag="J1138_002935"
     CDS             3601..5583
                     /locus_tag="J1138_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-glucuronic acid
                     oxidase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase"
                     /protein_id="HBA8454704.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKSASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQTGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLNWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5580..6470
                     /locus_tag="J1138_002936"
     CDS             5580..6470
                     /locus_tag="J1138_002936"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBA8454705.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            6470..8122
                     /gene="arnT"
                     /locus_tag="J1138_002937"
     CDS             6470..8122
                     /gene="arnT"
                     /locus_tag="J1138_002937"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBA8454706.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVVIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYHQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            8119..8454
                     /gene="arnE"
                     /locus_tag="J1138_002938"
     CDS             8119..8454
                     /gene="arnE"
                     /locus_tag="J1138_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBA8454707.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            8454..8840
                     /gene="arnF"
                     /locus_tag="J1138_002939"
     CDS             8454..8840
                     /gene="arnF"
                     /locus_tag="J1138_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBA8454708.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAANHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8834..9100)
                     /gene="pmrD"
                     /locus_tag="J1138_002940"
     CDS             complement(8834..9100)
                     /gene="pmrD"
                     /locus_tag="J1138_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBA8454709.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(9210..10565)
                     /gene="menE"
                     /locus_tag="J1138_002941"
     CDS             complement(9210..10565)
                     /gene="menE"
                     /locus_tag="J1138_002941"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBA8454710.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVKRQ
                     Q"
     gene            complement(10562..11524)
                     /gene="menC"
                     /locus_tag="J1138_002942"
     CDS             complement(10562..11524)
                     /gene="menC"
                     /locus_tag="J1138_002942"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBA8454711.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALEQLL"
     gene            complement(11524..12381)
                     /gene="menB"
                     /locus_tag="J1138_002943"
     CDS             complement(11524..12381)
                     /gene="menB"
                     /locus_tag="J1138_002943"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBA8454712.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12396..13154)
                     /gene="menH"
                     /locus_tag="J1138_002944"
     CDS             complement(12396..13154)
                     /gene="menH"
                     /locus_tag="J1138_002944"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBA8454713.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTGLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13151..14821)
                     /gene="menD"
                     /locus_tag="J1138_002945"
     CDS             complement(13151..14821)
                     /gene="menD"
                     /locus_tag="J1138_002945"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBA8454714.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14910..16205)
                     /gene="menF"
                     /locus_tag="J1138_002946"
     CDS             complement(14910..16205)
                     /gene="menF"
                     /locus_tag="J1138_002946"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBA8454715.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16284..16589)
                     /gene="elaB"
                     /locus_tag="J1138_002947"
     CDS             complement(16284..16589)
                     /gene="elaB"
                     /locus_tag="J1138_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBA8454716.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16644..17105)
                     /locus_tag="J1138_002948"
     CDS             complement(16644..17105)
                     /locus_tag="J1138_002948"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA8454717.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSETLRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            17170..18087
                     /gene="rbn"
                     /locus_tag="J1138_002949"
     CDS             17170..18087
                     /gene="rbn"
                     /locus_tag="J1138_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBA8454718.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(18208..>19917)
                     /locus_tag="J1138_002950"
     CDS             complement(18208..>19917)
                     /locus_tag="J1138_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA8454719.1"
                     /translation="SLILSGCGPEPEDKKSQQQQPSTPSDQQVLVAQQAAIKEVEQSA
                     AAAKAAADAKALAQQEVQQYSDKQTLQGRLQEAPTFARAAKANATHIANPGTARYQQF
                     DDNPVKQVAQNPLVTFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEVVNYFPSDWDI
                     KDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLVFLIDTS
                     GSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKVEINAAID
                     SLDAEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQ
                     RESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVA
                     KDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFELTLKG
                     QKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKYPQGNESQLVEFPLAFAIKAPSEDM
                     RFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFIRLIGLAKDLDN
                     SQN"
BASE COUNT         4699 a         5266 c         4984 g         4968 t
ORIGIN      
        1 cacacaaaaa taataataaa tgtagttatc atcgcaaaag agattactgt cactttcaca
       61 tatttctgag ttaccgtatt ctcatggttt ctttttattt ataaggaata cccctgtgcg
      121 acaacggacc attgtatgcc ctttgattca aaatgatggt gcttatttgc tgtgtaaaat
      181 ggccgacgat cgcggcgttt tccccggtca atgggcgctt tcgggtggcg gcgtggagcc
      241 tggcgaacga attgaagagg cactacgccg cgaaattcgc gaagaactgg gagaacagct
      301 gcttttgaca gaaatcacgc cgtggacctt cagcgatgat attcgcacca agacgtatgc
      361 agatggtcgc aaggaagaga tttatatgat ttacctgatt tttgactgcg tttctgccaa
      421 ccgagatgtg aaaataaacg aagagtttca ggactacgcg tgggtaaaac ctgaagatct
      481 ggtgcattat gatttgaatg ttgccacccg aaaaacgtta cgtttgaaag gtcttcttta
      541 acaatgatga cagccatcac tggctgtcag attgaaaatt tagtggttaa cgaattcccc
      601 atccagataa actttgcctt tttccacatg catgactaaa ccatccagat aatcaggttt
      661 aaatgtcgca tcacgcttat ctttagcaat atatgtcagg caatgattat gggtaaaaat
      721 aacgatattt ttatcaggtg ctttgcgttg taagtcttta attgcactat aaatctcatt
      781 accgcattga agaaatcgtt tatctaccgt cagcttttta cccgccgaaa accaggtcgc
      841 cgactgaata gtccggacgg tattactgga atagagatca aaatcaggga tatcagcact
      901 aaaagcgttg cccagttcac gggcatcctg ggtacctttg accgtaatac ctgttttatc
      961 tgacaagcat tgattggttg aacgatcgca acgttcagca tggcgaaaca aaacgacaac
     1021 cgggtgctgc tgtgccagtc tggccagtgt tttattgtcg atgcgtggca aaccattgct
     1081 actccaggcg gcatgagtac ccagtccggc aatcgcagcg agcgccagta aaatgatgaa
     1141 atattttttt gacttcaacg aagatcggca aaaagctaac acgtaaactc cacctgtaga
     1201 caagcgcaac cagacaatta ccgtgaaatt gagcgacttt tctggcgata attagcagtt
     1261 ggtgtaatat taaaaatcct atgatgccgg caaaatgcct caaaattttg ccaaatgcaa
     1321 agtctaaata agaaaaaata taaaaatttc aatatttacg tctaatatta gtttcttaag
     1381 gttaagttaa tattctatcc ttaaaatttt gctccaaatg gcaaaatata cacaacactc
     1441 tttatagcaa atataagtgg acaggtattc aatgacggat ggaaaaaaaa tgtcaggatt
     1501 tttgcctttt tcgcgaccag caatgggcgt agaggaactc gctgcagtta aagaggttct
     1561 cgaatccggt tggatcacaa ccggtccgaa gaatcaggcg cttgaacaag ctttttgcca
     1621 gttgacggga aatcagcatg ccatcgcggt cagttcagcc accgccggaa tgcatatcac
     1681 gctaatggcg ttggaaattg gcaagggcga tgaagtgatt acgccttccc tgacctgggt
     1741 ttcaaccctc aatatgattt ccttgctggg tgcaacgccg gtaatggtgg atgtcgaccg
     1801 cgatacgctg atggtcacgc ctgaagcgat cgaagcagcc attacgccac gcactaaagc
     1861 catcattccg gtgcattatg ccggtgcgcc agcagatatt gacgccattc gcgccattgg
     1921 cgaacgttac ggcatcgcag ttatcgaaga tgctgcccat gccgtcggta cgtattacaa
     1981 agggcgacat attggcgcaa aaggtactgc tattttttcg tttcacgcca tcaaaaatat
     2041 tacctgtgcg gaaggtggcc tgattgttac tgataatgaa aatcttgccc gccagctgcg
     2101 gatgctgaaa tttcacggtc tgggtgtcga tgcctatgac agacatacct ggggccgtgc
     2161 accgcaggct gaagtcttaa cgccgggcta taagtacaat ctgaccgata ttaacgccgc
     2221 gattgccctg acacagttag tcaaattaga gcacctcaac acccgtcggc gcgaaattgc
     2281 ccagcaatat cagcaagcac tggcagctct cccctttcag ccattaagcc ttcccgcctg
     2341 gccgcacgtt cacgcctggc atctgtttat tattcgtgtc gatgaacaac gttgtggtat
     2401 cagtcgcgat gcgttgatgg aagcgttaaa agaaagaggc attggtaccg ggttacattt
     2461 ccgcgccgct cacacacaaa aatattatcg cgagcgtttt cccacgctgt cgttaccgaa
     2521 taccgaatgg aatagtgaac gcatctgttc gttgccgctg ttcccggata tgaccaccgc
     2581 cgatgccgac cgcgtcatca cagcccttca gcaactcgca ggacaatgag ccatgtttga
     2641 aatccaccct gttaagaaag tctcggtggt tattcccgtt tataacgagc aggaaagctt
     2701 accggaatta atcaggcgca ccaccgcagc ctgtgaatca ctggggaaag agtatgaaat
     2761 cctgctgatt gatgacggca gtagcgataa ttccgcgcat atgctggtcg aagcctcaca
     2821 agcggagggc agccatattg tgtctatctt gcttaaccgc aattacgggc aacattcagc
     2881 gattatggcg ggattcagtc acgtcaccgg cgacttaatt attaccctcg atgccgatct
     2941 ccagaatccg ccagaggaaa tcccccgtct ggtggcaaaa gccgatgaag gttacgacgt
     3001 ggtagggact gtacgccaga accgccagga cagctggttt cgtaaaaccg cttcgaagat
     3061 gattaaccgg cttattcagc gcaccactgg caaagcgatg ggtgactatg gttgtatgct
     3121 gcgcgcctat cgccgtcata ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt
     3181 tatcccgatt ctggcgaata tcttcgcccg ccgtgccatt gaaattccag tgcaccatgc
     3241 cgagcgtgag tttggtgaat ccaaatacag ctttatgcgc ctgattaatt tgatgtacga
     3301 cctggtgacc tgccttacca caacgccgct acgtatgttg agtttgctcg gcagcattat
     3361 tgcgattggc ggttttagca ttgcggtgct gctggtgatt ttacgcctga ccttcggacc
     3421 acaatgggcg gcagaaggcg tctttatgct atttgccgtg ctgtttactt ttattggcgc
     3481 tcagtttatc ggcatgggat tgctcggtga atatatcggc aggatctaca ccgatgtccg
     3541 cgcccgcccc cgctattttg ttcagcaagt tatccgtcca tccagcaagg aaaatgaata
     3601 atgaaaaccg tcgtttttgc ctaccacgat atgggatgcc tcggtattga agccctgctg
     3661 tctgccggtt acgaaattag cgccattttt acccatactg ataaccccgg tgaaaaagcc
     3721 ttttatggtt cggttgcccg actggcagcg gaaagaggca ttccagttta tgcgccggat
     3781 aacgttaatc atccgctgtg ggtggaacgc attgcccaac tatcaccaga ggtgattttc
     3841 tctttttatt atcgccatct tatttgcgat gagattttgc agctcgctcc gagaggtgca
     3901 tttaatctgc atggttcgct gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg
     3961 ctggtgaacg gtgaaacgga aactggcgtt acattgcacc gaatggtgaa acgtgccgat
     4021 gccggggcca ttgtggcgca actgcgcatt gccattgcgc cagacgatat cgccattacg
     4081 ctgcatcata aattgtgcca tgccgcgcgc cagctactgg aacagacatt acccgccatt
     4141 aaacacggta atattctgga aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc
     4201 agaacgccgg atgacagttt cctcgaatgg cataagtcgg catccgtact gcacaacatg
     4261 gtgcgtgccg ttgccgatcc gtggccgggt gccttcagct atgttggcaa tcagaaattc
     4321 accgtctggt cgtcgcgtgt tcatcctcat gccagcaaag cacagccggg gagcgtgatt
     4381 tctgttgcac cactgctgat tgcctgtggc gatggcgcgc tggaaatcgt cactggacag
     4441 acgggcgacg gcattactat gcagggctcg caattagcgc agacgctggg cctggtgcaa
     4501 ggttcacgct tgaatagcca gcctgcctgt gccgcccgac gccgtacccg ggtactcatc
     4561 ctcggggtga atggctttat tggcaaccat ctgacagaac gcctgctgcg cgaagatcat
     4621 tatgaagttt acggtctgga tattggcagc gatgcgataa gccgttttct gaatcatccg
     4681 cattttcact ttgtcgaagg cgatatcagt attcattccg aatggattga gtatcacgtc
     4741 aaaaaatgtg atgtcgtctt gcctttggtg gcgatagcca cgccgattga atatacccgc
     4801 aacccgctgc gcgtatttga actcgatttc gaagagaatc tgcgcattat ccgctactgc
     4861 gtgaagtacc gtaagcgaat catcttcccg tcgacttcag aagtttatgg gatgtgtagc
     4921 gataaatact tcgatgagga ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc
     4981 tggatttatt cggtgtctaa acaattactt gatcgagtga tctgggccta tggcgaaaaa
     5041 gaaggtttac agttcaccct cttccgcccg tttaactgga tggggccacg actggataac
     5101 cttaatgcag cgcgaattgg cagctcccgc gctattacgc aactcattct caatctggta
     5161 gaaggttcac cgattaagct gattgatggc ggaaaacaaa aacgctgctt tactgatatt
     5221 cgggatggta tcgaggcgtt ataccgcatt atcgaaaatg cgggaaatcg ctgcgatggc
     5281 gaaattatca acattggcaa tcctgagaac gaagcgagca ttgaagaact gggggagatg
     5341 ctgctggcga gcttcgaaaa acatccgctg cgccattact tcccaccgtt tgcgggcttt
     5401 cgtgttgtcg aaagtagcag ctactatggc aaaggatatc aggacgtaga gcatcgtaaa
     5461 ccgagcatcc gcaatgcccg ccgctgcctg aactgggaac cgaaaattga tatgcaggaa
     5521 accatcgacg aaacgctgga tttcttcctg cgcaccgttg atcttacgga taaaccatca
     5581 tgaccaaagt cggcttacgc attgatgtcg atacctttcg tggcacccgt gaaggcgtgc
     5641 cgcgtctgct ggaaatcttg agtaagcata atattcaggc cagcattttt ttcagcgtcg
     5701 gcccggacaa tatgggccgc catctctggc gactggtgaa gccacagttt ttgtggaaga
     5761 tgctgcgctc aaacgcggca tcgctttatg gctgggatat tttactggca ggtacggcct
     5821 ggccaggtaa agagattggt catgccaatg ctgatatcat tcgtgaagcg gctaaacatc
     5881 acgaagtcgg cctgcacgcc tgggatcacc atgcctggca agcccatagc ggtaattggg
     5941 atcggcaaac aatgatcgac gatattgcac gcggccttcg caccctggaa gagattatcg
     6001 gtcaaccggt aacctgttct gccgctgctg gctggcgtgc tgaccaacag gtgatcgaag
     6061 caaaagaagc gttccatttg cgctacaaca gcgattgtcg tggggcgatg ccgtttcgtc
     6121 cattgctcga atctggaacc cccggcactg cgcaaattcc ggtgacctta cccacctggg
     6181 atgaagtgat tggtcgggat gtgaaagcag aagattttaa cggttggtta ctcaaccgca
     6241 tccagcgaga taaaggcacg ccggtttata ccattcatgc cgaagttgaa ggctgcgctt
     6301 atcagcataa ttttgtggat ctcctgaaac gcgcagctca ggaaggcgtg acattttgcc
     6361 ctttaagcga actattatca gggacgttgc cgctcggaca agttgttcgc ggaaatattg
     6421 ccggacgtga aggctggctg ggttgccaac aaatggcggg tagtcactga tgaaatcggt
     6481 acgttacctt atcggcctct tcgcatttat tgcctgctat tacctgttac cgatcagcac
     6541 gcgtctgctc tggcaaccag atgaaacgcg ttatgcggaa atcagtcggg aaatgctggc
     6601 atccggcgac tggattgttc cccatctgtt agggctacgt tatttcgaaa aacccattgc
     6661 cggatactgg attaacagca ttgggcaatg gctatttggc gcgaataact ttggtgtgcg
     6721 ggcaggcgtt atctttgcga ccctgttaac tgccgcgctg gtgacctggt ttactctgcg
     6781 cttatggcgc gataaacgtc tggctttact cgccacagta atttatctct cattgtttat
     6841 tgtctatgcc atcggcactt atgccgtgct cgatccgttt attgccttct ggctggtggc
     6901 gggaatgtgc agcttctggc tggcaatgca ggcacagacg tggaaaggca aaagcgcagg
     6961 atttttactg ctgggaatca cctgcggcat gggggtgatg accaaaggtt ttctcgccct
     7021 tgccgtgccg gtattaagcg tgctgccatg ggtagcaacg caaaaacgct ggaaagatct
     7081 ctttatttac ggctggctgg cggttatcag ttgcgtactg acggttctcc cctggggact
     7141 ggcgatagcg cagcgggagc ctgacttctg gcattatttt ttctgggttg agcatattca
     7201 acgctttgca ctggatgatg cccaacatag agctccgttc tggtactact tgccggtcgt
     7261 cattgccggt agcctgccgt ggctgggatt actccccggt gcactgtacg ctggctggaa
     7321 aaaccgcaag cattccgcaa ccgtctattt gttgagctgg acgataatgc cgctgctgtt
     7381 tttctccgtc gctaaaggca aattgcccac ctatattctt tcctgctttg cacctctggc
     7441 aatgctgatg gcgcattacg cattgctggc tgcaaaaaat aatcctctgg cgttgcggat
     7501 taatggctgg attaatatcg cttttggcgt cactggcatt attgccacat ttgtggtctc
     7561 cccctgggga ccaatgaaca caccggtgtg gcaaaccttc gagagctata aagtcttttg
     7621 tgcctggtct attttttcgc tatgggcatt tttcggctgg tacaccttaa caaacgtcga
     7681 aaagacctgg ccttttgccg cgctttgccc gctggggctg gcgttgctgg taggattttc
     7741 aattcctgac agagtcatgg aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga
     7801 atcacttcag ccaagccgct atattcttac tgatagcgtc ggtgttgccg caggtctggc
     7861 atggagcctg caacgcgatg acatcatcat gtatcaccag acaggtgagt tgaaatatgg
     7921 ccttaattat ccggatgcaa aagggagatt tgtcagcggt gatgagttcg caaactggct
     7981 taatcaacat cgtcaggagg ggattattac actcgtgctt tcggttgacc gcgatgaaga
     8041 tatcaatagt ctcgccattc cgcccgcaga tgtcatcgat cgtcaggagc gtctggtgct
     8101 aattcagtat cggcccaaat gatctggtta acattagtct ttgccagctt gcttagcgtt
     8161 gccgggcagt tgtgtcagaa acaggcaacc tgctttgcgg cggtaaacaa acggcgcaaa
     8221 catatcgtgc tgtggctggg actggcgctg gcttgtcttg gccttgccat ggtgctctgg
     8281 ctgctggtct tgcagaacgt accggtaggc attgcctacc cgatgttaag tctgaatttt
     8341 gtctgggtaa cgctggctgc agtaaaactg tggcacgagc cggtatcgct gcgtcactgg
     8401 tgtggggtgg cgttcattat tggcggcatt gtgatcctcg ggagtacggt gtaatgggcc
     8461 tgatgtgggg attattcagc gtcattatcg cctccgctgc gcaattaagc ctgggttttg
     8521 cggcgaatca tctgccgcca atgacgcact tatgggattt tatcgcggcg ctgttagctt
     8581 ttggcctgga tgcccggata ctgttactcg ggctgctggg ttatctgctt tccgtttttt
     8641 gttggtataa aacactacat aaacttgcgc taagcaaagc ctatgccttg ctcagtatga
     8701 gttatgtgct ggtatggatt gcttcaatgg tacttcccgg ctgggaaggg actttttcgc
     8761 tgaaagcact actgggagta gcctgtatta tgagcgggtt gatgctgatt ttcctgccca
     8821 caacaaaaca acgttactga gttttccctg ccgctttaca ctgccgttcc cactcatcgg
     8881 ggctataaca gcttgcagaa aggactttca ccgtgtgcag cttttcacga ttaatacagt
     8941 aaagcgcatt ctgcaaaggc gagagtaaat cccccacttt cacggcgaag tcactcttca
     9001 cttcggcgat catttttatc gcgccgccag catcgcagag cataattaca tgtctgttat
     9061 cttgtttatt gcagcacgat tttttgacca gccattccat tgcattatcc tgtttgctaa
     9121 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
     9181 cagaaatgag aagtgaaacg gtgggttaat tattgctggc gtttcaccca ctcttttagc
     9241 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggcagagt cagccagcgc
     9301 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgtcaagatc aacgctttgc
     9361 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacgggaacg
     9421 ataaacacct gcaaaaccgc aggatgtgca gcaataacgc gctcgacttc ttccggctga
     9481 ataccctcac cgccactgaa gaatagattg tctaaacgtc cgacaatggt caacttgcca
     9541 ttatgcatct caccgcgatc gcgcgtggcg taccagcctt cgtcattaac cagtggaacc
     9601 cgttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatca
     9661 ttaacgattt tcacttcccg acccggcagc ggcgaaccaa cgtctgccag accgtcggct
     9721 tctttcgcac acactgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaccgaatc
     9781 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaaaacc
     9841 gctttcaggg aaacggaact acggttaacc agcaaacgcc agagttgtgt tggcaccagt
     9901 gaagcgtgag tacagcctgc cagcatctgc tccaatggct gtttatcacg caccgtcatc
     9961 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    10021 aaagagagca accagtcatc gtgatcacca aaaggaatca gcgataacac cccttcggcg
    10081 ctggcaagat gagcctgata agtatgaaca gcggctttcg gcaagccagt ggaaccagag
    10141 gttaacgtca ttgagcacag acgcgtcggc tgccacgccg cggcatgtgc gccttcaacc
    10201 agctgaatgt gcagcgacgc taacgccgga aacgtgtttt ccccttccgg caccagcgca
    10261 aattgcagtg taagattggg cagcaattcc tcaagcagcg gttgcggcag ctgagggttc
    10321 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggccagcag cgtttgcggc
    10381 gtattccacg cccgcaacat cacgccgctg ccctcaacca caccctgcac cgcaaagctg
    10441 gaggctaatt catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    10501 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    10561 atcataacag ctgctccagt gcatcaacgt ccactaacgg cagcaggctc cccggccagc
    10621 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    10681 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    10741 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgcac ttgctcgcgt actttatcca
    10801 ggctgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gctacaaagg
    10861 caaaatccgg ctcgcgcaag ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    10921 acgcacgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    10981 aatccgggtt aacgtatttg gcaaactgct ggcctttcag cggtgtccag gcgcggttcg
    11041 cgtcaagacg caaatgcaga tcaggaattg cctccagcaa cagattcacc accatgccgt
    11101 cgcgcacggc ttcgtacaat cccactttga ccttcgccac tttctcgcca ggcatatcgg
    11161 caagtttgag gatcagatcg tcaggatcgc cattacacag cggtgccgca cggtaattgg
    11221 ccgcctgcgg gagcgtatct gccagctccg ccaatgcaca acttacgcca aaggcaacgg
    11281 aaggcatctg cggtaattcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    11341 cactttgcgc ctcttcccag gtttcctgac tgaagcccgg cagtggggag atctcccccc
    11401 acccttcgcg ctcgccctca cgcaggcaaa catacagccc gtcgcgggtt tttaaccgcc
    11461 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    11521 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    11581 gcgaccttcc tgaccttctt ccgtcatata gaacagcatg gtggcgttgc ccgccagctc
    11641 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagtgc
    11701 catcgggctg ttttgcaaca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    11761 cagcggtaca acggtgttca ccagacccat atccagcgcc tgtttcgcgt cgtactgacg
    11821 gcacaggaac cagatttcac gcgctttttt ctgtccaacg atgcgagcca tgtaggaagc
    11881 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    11941 agccgcgata gtcaggtcgc acatcatgtg caggacgtga ccgccaccga tggagtagcc
    12001 agccaccatc gcgacaaccg gttttggaca ggtacggatc tgacgctgga agtccagcac
    12061 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gtactttctg
    12121 gtcaccaccg gagcagaacg ctttatcgcc tgcaccagtc agaatgatca cgccgatgtt
    12181 gtcgtcataa cgcgcatccg ccagcgcctg gatcatctct tttaccgtca gaggacggaa
    12241 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    12301 ataacgaatg tcctcaaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaagcat
    12361 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    12421 cttgcgatta cgccagcggg attttcccgg tgcgcgttat gtccggcgcg aggaatgaca
    12481 tggcagtcgg cagccagttc cgccgccaga gcgcggaatt tgctgtcacg ttcaccacat
    12541 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcggg ctggacggcg
    12601 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    12661 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccaatcg
    12721 gcaaataccg ccgttaacgg ttctgtacga aaacgctgcg cccattggcg atcggaacgc
    12781 tgacgttccg cgcgttgttc agcattttgc aatccaggat gcccgccttc gacaacaact
    12841 ccacaaagcc ccgccagctc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    12901 taccccacca gccagaagtt aaggatgttg taactaacca gcgttttaca caataagccg
    12961 gtgacatcat caaatccatc gacgctgatc gtcgctgaac caccgtgacc tgggagatca
    13021 acatacaacc gcgagtagtc ggcaaacgcc tcgcccactt cttgccattc atggcaatcg
    13081 ccagaaaaac cgtgcaaaaa caccaaccag ggtaaacctg gtttgccgtg ttttgcctgc
    13141 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    13201 cggtgtcgtt aaccaccatt tcaatcaccg tggtggtcgg cgtacgccag gcatcgacta
    13261 gtgtcgtttc aagttcctgc cagttttgcg gacgatgata tttcagctcg aacatcgcgg
    13321 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcgcgc tcgcttttcg
    13381 gcgttggcaa cagcgaaaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    13441 gtgcggaaac ctggcgcaat aacgccagcg cgttgagatc gtaaagtgcg gagagatcgc
    13501 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gccggcggca gtcgagagca
    13561 gcccgtcgat accgctggca ccacggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    13621 aaagcgcatc aatcagacgc accaccaggc tgttaccgac aaacaattgc ccctgttcag
    13681 gcaagtagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatcg cgacgggcaa
    13741 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    13801 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    13861 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    13921 agcttgcctg ccattggagg agtcgtttcc ccgtcaggct gcttcccagt tgcaccacta
    13981 tttgtgcctg ttgcagttcg ctggtcgctt tggcattgcc taaccagaga tcggcacacg
    14041 gcagcggctg tccggtttgc gacagcacgt cgccaatcag cggccagcca agagtttgcg
    14101 cccacagtgc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    14161 gcttttgtcg ccagaagaac cagtcacgct gtttttcact ttcccgacga ggcgcttcac
    14221 gcagccacgg tttgtcgtcc tgccaccagt cgcccagccg ctgttgccag ctaatcccgg
    14281 tgtcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccaccag
    14341 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    14401 gggtcgggcg cggcagtgaa atactgtgcg tggggtgaga ggcgaacatt cccggctgac
    14461 gaatcgcctg attcgcgccg cagtcaatca gctccggcgg gcgatcggcg gttaacagga
    14521 tcagtttttc tccggttaac ccagcttcaa tcagtgccgg atagagattt gctaccgccg
    14581 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagtgcca
    14641 gatgccccag tccacgctca tcgaaatggg tatgatgaat aaatgcggaa ttctccgccg
    14701 ccgctaacgt taacggtgta gaacgcgagc caggggcgat acagatgtgt ctgacgccgt
    14761 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    14821 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    14881 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    14941 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgcct
    15001 gcgccagcat atagccgcac gatattgccg ctgatttttg ctgagcgcag ggaaacgcag
    15061 aattcacttt gctgtaatga gagatagccc gcagaaccgg cgtaccattc tcgagtgaac
    15121 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    15181 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    15241 cagcggcgaa gatgctgtac tttacgtaga cgcagtacct gcggcggtaa aacatccagc
    15301 gtttgggtat cggcctgcaa tcgttggcag atatcttcca ccaccaacat gttctcgcgc
    15361 tggtttttat catccgccat cagccactca cctaactgct gcgcctgctt atcatcagga
    15421 tgatttgcta ccgttcccgc cagcgcttcg gtacgcagcg ctttgtcacg ccgccgccat
    15481 aaccgttccg gtgaagaacc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    15541 tagcaattca gattgagccg acgactggca gccatcatcg ctgccgcatt gaccggactt
    15601 gcgaaatgca ggtcggttgc ccgagcaagc acaactttgt cgagctcgcc ttcggcgatg
    15661 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    15721 cgcgtggtgg ttaaatgtaa tccaggcagg ggcttgatac tcaccagtgt ggcgataaat
    15781 tcttttgcct tgattgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    15841 gtggctttac cgccacaacg tcgccattcc aggcgtggta aaagtaaatt gccctgcgac
    15901 gggtcaaacg cattcaaccc ccagatgcgt aagtcggcgt gttccgggtg ctggcgaaga
    15961 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cacccaggac ggcagcttct
    16021 tcatcaccat tacgttgttg ccagtagaat tgcggataca cctgctgact ggccagccag
    16081 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    16141 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    16201 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtacgac aaaaaaaatg
    16261 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcccag aaccagccca
    16321 acggccgcac ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    16381 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    16441 acatcatcca gcgctttttc tgcacgcgct ttcagctcaa catatttctg atcggcggga
    16501 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcatcatca
    16561 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgtgtaa aacctgtcac
    16621 taactataga caagggttat tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    16681 gggataccat cttcttcata aatatcggtc acagggatga aaccaaattg cccataaaag
    16741 ttttgtaaat gcgcctgcgc ccccagataa atcggcttct ccggccagcg gcgagtacac
    16801 gattccagcg ccttactcat caattgctga ccgatttttt caccgcgtaa ggtttcgctg
    16861 acaatcaccc gaccaatcac aacgggttgc agttcgtcgt cacttttcag aatcctcgca
    16921 tacgccacca gtgtcccgtt atgccaaccg agaatgtggc gattttctcc ctccagatcg
    16981 tcaccatcga tatcctggta aggacagttt tgttccacta caaataccgc acaacgcagt
    17041 tgtaataagg cataaagctg agaaacggat aaatcagaat gatgcagatc ttgccagtcg
    17101 atcatggaag gctcctttgt gctgtttgct ttatactagc cggaatattt tcagcagcgc
    17161 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgtacgcgt
    17221 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttattt
    17281 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    17341 gacaagattt ttatcagtca ccttcatggc gatcatctct ttggtttacc cggcttactg
    17401 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcacggtatc
    17461 cgtgaatttg tggagaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    17521 attgtcgaaa ttggcgctgg cgaaattttc gatgatggcc tgcgcaaagt gaccgcttat
    17581 ccgctggaac acccactgga atgctatggc tatcgtattg aagaacatga taaaccgggt
    17641 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc caggcccgct gtttcaggaa
    17701 ttaaaagcgg gcaaaacaat catgctggat gatggaaggc agattaatgg cgcagattac
    17761 ttagccgctc cagtgccggg taaagcgctc gctattttcg gcgatacggg cccctgcgat
    17821 gccgcgctgg agctggctaa aggcgtcgac gtaatggttc acgaagcgac tctggatatg
    17881 gcgatggagg ccaaagccaa cagtcgcggc catagttcta cacgccaggc ggcggcacta
    17941 gcccgtgagg ctggagtcgg taagctaatc attacccacg tcagctcgcg ctatgatgac
    18001 aaaggttgtc agcacctgtt acgtgaatgt cggtcaattt tcccggcgac tgaactggcg
    18061 aatgatttcg cggtgtttag catttaagtc gaaagcggtg gttagttagc agattaattt
    18121 aacgatacca acgcatttat ttgacatttt ttcatctgtt tgccggatac gtttgatgtc
    18181 gatatcaggc aaacagaatt atcattttta gttttgtgaa ttgtccagat ctttcgccag
    18241 tccaatcagg cgaataaatt ccgctctgta accctgtgga tcttcccctt ttgccttctg
    18301 agcccactgt ttgatctgct gccaggaagt attattcagg tattcagaac cgcgtaactt
    18361 ttgcccatat gcagccactg ctgcgcgaaa acgcatatct tctgaaggtg cctttattgc
    18421 gaatgccaac gggaattcaa ctaactgact ttcgtttccc tgcgggtatt tccaacgaat
    18481 ctttaaccag gcaagttctt ttgttttgtc cgatttcgct gatttgttat ccggggcata
    18541 gcgtaactta tcaattgatg ctttttgccc tttcagcgtt aattcaaata acaatgttat
    18601 atgtttacct gcgccaatat cacccgcatc aacgttgtcg ttattaaaat cctctgccct
    18661 aagttggcgc ttttcataac ctatctgacg gtattccgtt acccaagcgg ggttaaactc
    18721 aatttgcgct ttgacatctt ttgctacggt aatcaacgtc tgccgcattt cactgttcaa
    18781 cactttctgc gcctctgaga gggtatcaat gtagctatag ttgccgttac caacatcggc
    18841 aattcttacc atcattgcct cgttataatt gctatccccc acgccaagcg tcgacagagt
    18901 aacaccggac tcacgctgtt ttttgaccat tgattcaatc gattttggat cgtcaatgcc
    18961 aacgttaaag tcaccgtcag tggccaataa aatgcgattg ataccacctt taataaaccc
    19021 cttcgctgcc tgctgataag ccatctccag cccggcaccg ccattggtac tgccttctgc
    19081 atccagcgaa tcaattgcag cattaatttc caccttgtga ctaccggaga tagaaggcaa
    19141 cgcaatacga gagtcgccag cgtaggtcac gatggcaatg ttatcctgct cacgaagttc
    19201 tttgaccaat aatttcaacg aagactggat aagtggcaaa cgttcatcag aaatcattga
    19261 accagaagtg tcgataagaa agaccagatt agaagcgggt aactcttcac ttttgcgatc
    19321 tttcgccagg atatcaactt tcagcaatgt tcgctgttca ttccagggtg caggtgccaa
    19381 ttcgtagcgc atagcgaaag gtattggctt agaggccgga atagattgtt tgtctttgat
    19441 atcccagtca gacgggaaat aattgactac ctcctccacc cgcacagcgt ctggcggagg
    19501 taacagccct tgattaagga aacgccgcac attcgcataa ctgccggtgt cgacgtcaag
    19561 gctaaacgtc acgagtgggt tttgcgctac ctgcttaacc ggattatcat cgaactgctg
    19621 gtagcgagcg gttcctggat ttgcgatatg tgtagcgttt gctttagccg ctcgtgcaaa
    19681 tgttggcgct tcctgcaatc tcccctgcaa agtctgtttg tctgaatatt gttgcacttc
    19741 ttgctgggct aaggctttcg cgtctgcagc agctttagcg gcggcggcgc tttgctctac
    19801 ctcttttatt gcagcttgtt gcgcgacaag cacttgttgg tctgaggggg tactgggttg
    19861 ttgttgctga cttttcttat cctcaggttc cggcccacat cctgacaaaa tcaaact
//
LOCUS       DADTFG010000089        19664 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.089, whole genome shotgun sequence.
ACCESSION   DADTFG010000089 DADTFG010000000
VERSION     DADTFG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.089"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            73..615
                     /locus_tag="J1138_002951"
     CDS             73..615
                     /locus_tag="J1138_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454720.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            715..1917
                     /locus_tag="J1138_002952"
     CDS             715..1917
                     /locus_tag="J1138_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBA8454721.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLSGQNIASEHGWIEV
                     VESMTLSV"
     gene            2137..2919
                     /locus_tag="J1138_002953"
     CDS             2137..2919
                     /locus_tag="J1138_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBA8454722.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2934..4139
                     /gene="rhmD"
                     /locus_tag="J1138_002954"
     CDS             2934..4139
                     /gene="rhmD"
                     /locus_tag="J1138_002954"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HBA8454723.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYAIKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4196..5485
                     /locus_tag="J1138_002955"
     CDS             4196..5485
                     /locus_tag="J1138_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8454724.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5503..6306
                     /gene="yfaU"
                     /locus_tag="J1138_002956"
     CDS             5503..6306
                     /gene="yfaU"
                     /locus_tag="J1138_002956"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HBA8454725.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILGVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(6347..6532)
                     /locus_tag="J1138_002957"
     CDS             complement(6347..6532)
                     /locus_tag="J1138_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454726.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLEGMHEQAIKIALRMLEQGIDRDQ
                     VLAATQLSEADLAANNH"
     gene            complement(6545..7504)
                     /locus_tag="J1138_002958"
     CDS             complement(6545..7504)
                     /locus_tag="J1138_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBA8454727.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     KGKVEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCY"
     gene            complement(7697..8887)
                     /gene="glpC"
                     /locus_tag="J1138_002959"
     CDS             complement(7697..8887)
                     /gene="glpC"
                     /locus_tag="J1138_002959"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBA8454728.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8884..10143)
                     /gene="glpB"
                     /locus_tag="J1138_002960"
     CDS             complement(8884..10143)
                     /gene="glpB"
                     /locus_tag="J1138_002960"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBA8454729.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10133..11761)
                     /gene="glpA"
                     /locus_tag="J1138_002961"
     CDS             complement(10133..11761)
                     /gene="glpA"
                     /locus_tag="J1138_002961"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBA8454730.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTTANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQTLHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKMRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12034..13392
                     /gene="glpT"
                     /locus_tag="J1138_002962"
     CDS             12034..13392
                     /gene="glpT"
                     /locus_tag="J1138_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBA8454731.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13397..14473
                     /gene="glpQ"
                     /locus_tag="J1138_002963"
     CDS             13397..14473
                     /gene="glpQ"
                     /locus_tag="J1138_002963"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBA8454732.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNVLEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14676..15326
                     /gene="inaA"
                     /locus_tag="J1138_002964"
     CDS             14676..15326
                     /gene="inaA"
                     /locus_tag="J1138_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBA8454733.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15380..15634)
                     /gene="yfaE"
                     /locus_tag="J1138_002965"
     CDS             complement(15380..15634)
                     /gene="yfaE"
                     /locus_tag="J1138_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBA8454734.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15634..16764)
                     /gene="nrdB"
                     /locus_tag="J1138_002966"
     CDS             complement(15634..16764)
                     /gene="nrdB"
                     /locus_tag="J1138_002966"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBA8454735.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(16954..19239)
                     /gene="nrdA"
                     /locus_tag="J1138_002967"
     CDS             complement(16954..19239)
                     /gene="nrdA"
                     /locus_tag="J1138_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBA8454736.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4511 a         4865 c         5364 g         4924 t
ORIGIN      
        1 aatcctctgc gccaggcagt acaatcccgc agctttggga attttgatta attgcttgtg
       61 ggaaagagga taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct gttggtttct
      121 gcatcggtca atgcaatgag catcagcggc caggcgggta aagaatacac caatattggt
      181 gtcggttttg ggactgaatc gacgggcctg gcgttaagcg gtaactggac acataacgac
      241 gacgacggtg atgccgcggg cgtggggctg gggttgaatc tgcctctcgg gccattaatg
      301 gcgaccgttg gcggaaaagg cgtttacacc aacccgaacg atggtgatga aggttatgca
      361 gcggcagttg gcggtggttt gcagtggcaa attggtgaca gcttccgctt gtttggtgag
      421 tattactact ctccggattc gctctccagc ggaattaaaa gttatgaaga agcgaatgct
      481 ggcgcgcgtt acaccattat gcgtccagtc agcattgagg ctggctaccg ttacctgaac
      541 ctggcaggta aaaatggcaa tcgtgataac gccattgctg atggtccgta tgttggggta
      601 aatgccagct tctgacgcct tccggcgcgg cgttctgtcg cgccattcag tttcttacta
      661 cgccctgtct ccctttgcgt tatagtgatt cgataacaaa agcaggagat aaccatgtta
      721 aaagtggaaa tgttatccac cggggatgaa gtgttacacg ggcaaatcgt tgacactaac
      781 gctgcctggc tggccgattt tttctttcat caggggctgc cattatctcg ccgcaatacg
      841 gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca gcacgccgat
      901 gtgttgatcg ttaacggcgg gctgggaccg accagtgatg atttaagcgc actcgccgct
      961 gcgacagcaa aaggtgaagg cctggtgctg catgaagcct ggctcaaaga gatggaacgc
     1021 tatttccatg aacggggacg agtaatggca ccgagcaacc gtaaacaagc ggagctgcct
     1081 gccagtgctg aatttatcaa taacccggta ggcactgcct gtggttttgc catacagctt
     1141 aatcgttgcc tgatgttctt tacccccggc gtaccgtcag aatttaaggt gatggtcgag
     1201 cacgaaatcc tgccgcgcct gcgcgagcgt ttttctttac cacagccgcc ggtttgcctg
     1261 cgtttgacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga cactctacaa
     1321 ctgccgccgg gcgtaacaat gggctatcgc tcctcaatgc cgattatcga actgaaactc
     1381 accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt taaacgtgtt
     1441 gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat cagtcgcgaa
     1501 ttgcagagcc gccagttcag tctgacgctg agcgagcaat tcaccggtgg tttattggct
     1561 ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg aagtggttcc ttcacaggag
     1621 gaaaccctgg cgcaaactgc gcactggatt acagaacggc gggcgaacca ttttgctggg
     1681 ttggcactgg ctgtttctgg tctggaagat gagcatctca actttgcact agccacgcca
     1741 gacggcactt tcgctttgcg tgtacatttc agtgccacac gctacagtct ggctattcgc
     1801 caggaagtgt gcgcaatgat ggcgcttaat atgctacgcc gttggctgag cggtcagaat
     1861 attgccagcg agcatggctg gattgaggtt gttgagtcca tgaccttatc tgtctgattc
     1921 cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa tttgtccaac
     1981 ctgttgtact caataagaaa aaatccgtgc gctgattcac aaatttgtgc gtaatttctc
     2041 cagtccgctg gacgagaaac gggatttgat gcgcttcaca attttactcc ccctgagcaa
     2101 cgagactgat tccagtatct aagactggag tccagtatgt tggaatcaag caaagttccc
     2161 gccctgacac gggctattga tattctcaat ttgattgccc ggattggccc ctgtagtgct
     2221 gcgacgatca ttgacacatt ggggatccct aaaagcacag cctatttgct gcttaatgaa
     2281 ctcaggcgtc agcgttttct tagccttgat caccaggaaa atttttgttt gtggaccagg
     2341 ctggtggagc tttccggtca tgcgttaagc aagatggatc tccgggaact ggctcgaccg
     2401 cgtttgacgc aactgatgga taccaccggg ctgttatgtc atctggggat tattgataac
     2461 ggcagcgctt attacatttt gaaagtggag tcatcagcca ctatcagcgt gcgttcccat
     2521 gaaggaaaaa gcctttcgct ttatcgctcc ggaataggta aatgcctgct tgcctggcaa
     2581 cctgcagctg tacagcaaag tattatcgaa gggctggtct gggaacgggc gacacccaca
     2641 acgattactc atccacaacg gctctacgag gaactggcgc gtattcgccg tcagggctgg
     2701 agctatgaca acggcgaaga ttatgccgat gtccgttgtg tcgctgcgcc ggtgtttaac
     2761 gccaataacg agcttactgc tgcgatttcg gtggtcggta cccgcttaca aatcaatgaa
     2821 gaataccgtg attatctggc gggcaaggcc attgcctgcg ccagggatat ttcacgtttg
     2881 ttgggatgga aaagtccctt tgacttacag gcctcataac aatggagaac atcatgaccc
     2941 taccaaaaat caaacaggtt cgcgcctggt ttactggcgg tgcgacagca gaaaaaggcg
     3001 ctggcggcgg tgattatcac gaccaggggg cgaatcactg gattgatgat catattgcca
     3061 ccccgatgag taaataccgc gaatacgagc agtcccgcca gtcatttggc attaacgttc
     3121 ttggcacgtt gattgttgaa gttgaagcag aaaacggcca gaccggattc gccgtttcga
     3181 cagccggtga aatgggctgt tttattgtcg aaaaacatct taaccgtttc attgagggta
     3241 aatgcgtcag tgatatcaaa ctgatccacg atcaaatgct caatgccacc ctgtattact
     3301 ccggctctgg tggcctggtg atgaatacga tttcctgtgt cgatctggct ctgtgggatc
     3361 tgttcggcaa agtggtcggg cttccggttt ataaactttt aggcggcgct gttcgtgatg
     3421 agattcagtt ctacgccaca ggtgcgcgtc cggatctggc aaaagagatg ggctttattg
     3481 gtggcaaaat gccgacgcac tgggggccac atgatggtga tgcggggatc cgcaaagatg
     3541 ccgctatggt cgcggatatg cgtgaaaaat gcggtgagga tttctggtta atgctcgact
     3601 gctggatgag tcaggacgtg aactatgcca tcaaactggc ccacgcttgc gcgccctata
     3661 acctgaaatg gatcgaagag tgcctgccgc cacagcagta tgaaggttat cgcgaactga
     3721 aacacaacgc accagcgggg atgatggtca ccagcggtga gcaccacggc acactgcaat
     3781 ctttccgtac gctttcagaa accggtatcg acattatgca gccggatgtt ggctggtgcg
     3841 gtggcttaac cacgctggtg gaaattgccg caatcgccaa atccaggggg caactggtgg
     3901 tgccgcacgg ttcgtctgtt tactcccacc atgcggtgat caccttcacc aatacgccat
     3961 tcagtgaatt cctgatgacc agcccggatt gttcaacgat gcgtccgcaa tttgatccga
     4021 ttctgcttaa tgagccggtt ccggtgaatg gtcgtattca taaatcagtg cttgataaac
     4081 ccggtttcgg cgtcgaactc aatcgtgact gcaatttgaa acgcccctac agccactaat
     4141 cactctgggc gttgctgtga caacggtaac gcccacatat tctaaggagc tttttatgag
     4201 caccgctttg cttgacgccg tggtgaagaa aaaccgcgcg cgtttaattc cgtttatgtt
     4261 ggcgctgtat gtgctggcgt ttctcgaccg ttcgaatatt ggttttgcca aacagaccta
     4321 ccagattgat accgggctga gtaatgaagc ttatgcgctg ggagcaggca ttttctttgt
     4381 ggtatacgcg tttctggggg ttccggcgaa tcttttgatg cgcaaactgg gggcaagaac
     4441 ctggattggt acgacaacac tgctgtgggg atttctttcg gctgccatgg catgggccga
     4501 tactgaagcg aaatttctga ttgttcgcac tctgcttggt gctgcggagg ctgggttttt
     4561 ccctggtatg atttatctca cctcgcaatg gtttccgcag cgtaatcgcg ccagcattat
     4621 ggggctgttc tatatgggcg caccgctggc gttaacactg ggatcaccgc tttctggcgc
     4681 gctgctggag atgcatggat ttatggggca tcccggctgg ttctggatgt ttgtgattga
     4741 aggattgttg gcagtcggcg ctggggtatt cacattcttt tggcttgatg acacaccgga
     4801 gcaggcacgt tttctgagta aacaagaaaa aacgttgctt atcaatcaac tggcaagtga
     4861 agaacaacag aaagtgactt ctcgattgag cgatgcgctg cgtaatggcc gagtctggca
     4921 actggcgatt atctacctga ccattcaggt ggcggtttac ggattaattt tcttcctgcc
     4981 gacccaggtt gctgcattgc tggggacaaa agtgggcttt acggcgtcgg tggtcaccgc
     5041 cattccgtgg gttgcggcct tgtttggaac ctggcttatt ccgcgctatt ccgataaaac
     5101 cggcgaacgg cgtaatgttg cagcgctgac attactggcg gcgggcattg gtattggtct
     5161 gtccgggctg ctttctccag tactggcgat cgtagcgctg tgtgttgcag ccatcgggtt
     5221 tattgccgtg caaccggtgt tctggacgat gccgacacag cttctttccg gtacggcgct
     5281 ggctgcgggg attggtttta taaacctgtt tggtgcagtg ggcgggttta ttgccccgat
     5341 cctgcgcgtg aaagcagaaa cgttattttc cagcgatgcg gcgggattac tgacgctggc
     5401 agcggtggcg gtcatcggtt cgctgattat tttcactctg cgtgtaaatc gcactgttgc
     5461 gcagaccgac gtggcacatc attaaatagt ttaaggaaca cgatgaacgc attattaacc
     5521 aatcccttta aagaacgttt acgcaagggc gaagtgcaaa ttggtctgtg gttaagctca
     5581 acgactgcct atatggcaga aattgccgcc acttccggtt atgactggtt gctgattgac
     5641 ggggagcatg cgccaaacac cattcaggat ctttatcacc agctacaggc ggtagcgccc
     5701 tatgccagcc accccgtgat ccgtccggtg gaaggcagta agccgctgat taaacaagtc
     5761 ctggatattg gcgcgcaaac tctactgatc ccgatggtcg atactgccga tcaggcacgt
     5821 caggtggtgt ctgccacgcg ctatcctccc tacggtgagc gtggtgttgg ggccagtgtg
     5881 gcacgggctg cacgctgggg acgcattgag aattacatgg cgcacgtcaa cgattcgctt
     5941 tgtctgctgg tgcaggtgga aagtaaaaca gcactggata acctggacga aatcctcggc
     6001 gtcgaaggga ttgatggcgt gtttattgga cctgcggatc tttctgcctc gttgggctac
     6061 ccggataacg ccgggcaccc ggaagtgcag cgaattattg aaaccagcat tcggcggatc
     6121 cgtgctgcgg gtaaagcggc tggttttctg gctgtggctc ctgatatggc gcagcaatgc
     6181 ctggcgtggg gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta cagcgatgcc
     6241 ctggatcaac gactggcgat gtttaaatca ggcaaaaatg ggccacgcgt aaaaggtagt
     6301 tattgatatc aaaggcccat ggggatcggc tgtgggcctg tgttaattag tggttattcg
     6361 ctgccagatc ggcttcgctt agctgagtgg ccgcgagcac ctggtcacga tcaatgccct
     6421 gttccagcat gcgcaaggca attttaatgg cttgttcatg catgccttct aatttaccct
     6481 cctgccagcc aatttgatgt ccttcctgcc gtaaccgttc agcaatagtc attaatctct
     6541 ccttttaata acagttcgga atatcagcat cagtgagttg ggttgtcgcc agcacaatct
     6601 cacgttcaaa gccctgttcc agcatgcgca aggcaatttt aatggcttgt tcatgcatac
     6661 cttctacttt gcctttctgc aaaccctcca atttcccctc ctgccaacct tcaattttac
     6721 cttcctgcca accaatttga tgtccttcct gccgtagccg ttcagcaata gtcattaatc
     6781 tctccttttg tagtggtgaa cgttcggcaa tctcctgaat aaagcgggtg aaacgggagg
     6841 tatcgccgca ttgcagcaga taattaaaca gtgtttgtag ctgactgtca ttagtgaagc
     6901 ctctaaccaa aagcgtggtg atcctgtcga ccataccgat taaatcgcgg tcgcgaatat
     6961 gcttttgaat cagttccagc agagctatac gccgatgttg catgatctca tcgtcaggta
     7021 cgatggtgat atccaccaac ggaaaagctt cggtgtacaa ctgccgggca agttgcggat
     7081 cgtcaaactc atccagccag ttgagtgagt acgggtaggg cgaggcttcg ccatgataaa
     7141 acagcaatgg caccaccagc ggaactctgt catagccttt atccaggtga cgctgcatgg
     7201 cggcagtggc atagcgcatt agccgaaaag ccatattctt ttctgcagag ctttgatgtt
     7261 caatcacgca gtagatatag ccgtcaccct cgctggtttc cacggaccac aaaacatccg
     7321 agtagtaagc gcgtaaactt ttttcaataa aactggtggg ttccaggcgt aaggtttgca
     7381 ggttgcaaag cttgcgcagc ggctctggta gatgtatttc cagaaaatcc cgtgcggttt
     7441 cgggtgtgaa cataaaggtt ttaaacaccg catcatgcgg ggaggaggtt gttgattcgg
     7501 tcatggttgt ccgtcacctg atatttatga tgacgcgaca ttacggttat cccaggcctt
     7561 tgacatctgc cagtttcagc ctttgcgaga cgttttcaga ggaattttca ctgcgttgca
     7621 acgcatacat tgggcacgga atcgtgtacg caaaaatgcc cggtaacaac atgcttaccg
     7681 ggcatcagaa aggagtttaa gccagcgcct gtgccagcag cgtaatcgga tgttcgcagc
     7741 gaagacttgt ggacatctca atctgccatt tacaggtttc gcagtcggtg accaccagat
     7801 ctgcgccgct ctcttctatc tggcggaaca gtggtgcgcc gatggcttgt gaagtggggt
     7861 agttctcttt tttgaaaccg taagttcccg caataccgca gcactgggaa tccagcaccg
     7921 ttaactcaag ccccgggatt ttacgcaaca gctccagggt gtagagcgtc cagcccattt
     7981 tttccatatg gcacggagtg tgataaacca ctttcagcgg cagcggtttc agcggtaacg
     8041 ttttgccttc gtccagcttg cgccacagcc agcgggtcgc cagttcgata tgatcgcgca
     8101 agcctttgtt gtcgacattc agcacttccg ggtattcgtc gcgcagggca aatgtacaag
     8161 ttgaggaggt ggcaatcacc ggaatgcctt ttactcccac agcttcgcgg atcgactcta
     8221 cattcgtaat tgcctgtttg cgtgctttat cggtaaaacc gttggcgatc agcggtacgc
     8281 cgcagcattt ttctttgctg agcagttgta cgccggtacc catcgcgttg agcactttaa
     8341 ttaaatcttt gcctagctgc ggatggttgt agttaacgaa gcagccgtga aagaaagcga
     8401 cctggtcttt atactgcgct tgctgagccg ccacgctgcg ataccagcga cggaacgtac
     8461 cgaaggagta tttcggtagc gtgcggcggt ggtctatttt taacgccgca tcaagcaact
     8521 gccgcaccgg tttcagcgag gtagcggtgt tgacgatggg tgcgaacggc gtggaaacgc
     8581 tacccatcag gtcggtatga ctcaacacaa aattacgcag cgacgggcgt gtggtgtcat
     8641 atttcgcccg cgcgcgctgg ataatatcgc caatcttcac atcggacggg caggcgactt
     8701 cacaacgttt gcagttgatg caatatttca gcgcttcgtc atacagtgcg ccatctttta
     8761 aacgcagacg ctcgccatcc ggcccggctt gttttggccc cggataaccc ggattcaccc
     8821 ggctcaccgg gcaggcggtg gtgcagacgg tgcacttaat gcagttttcg aagctggtgt
     8881 cattcattgt tggcctcctg cgcgttgggc aatctgttga gcggcatgta aagcactgac
     8941 ggcacaaaca ccgccgccgc atccctgggc gatgggatca aatccgccca gcaccgaacc
     9001 gatggcaaac aggttctcaa tggtttgccc cgcctgcgac gggcgtagcg cctcatcagt
     9061 ggttacaccg aactgctgcc acggctgcgg cgcaaaaaaa tcgcccttat accattcacc
     9121 ccgcgtagcg gtttgtagca catcaaggcc gagaatcggc tcgcgaatgc cgtcacgttc
     9181 tgccaccagt ccgccgctaa agaaactgcc gctcgcaagc accgcaaatc gtgggcgcag
     9241 cgggatatcg gcgtgattgc gcgtccagat ttcattcact acgccatttt tacaggtcac
     9301 ttttttcact tcatcgcccg gcatccacac gccgccctgg cgcacaaact ggcgctgtaa
     9361 ctggttttgc agacgaatgc ccagcacgga aggcggcagc gttggcaaaa gcatcaatga
     9421 acaaggtagt ttttcattca accaatgcca cagtttgtca tcggccaggc cgaagcaggc
     9481 gggcatcagg atcatttcgc aggtattggc gaccggaata agcgcatcaa gtaacaacgg
     9541 ccagttttct tcattatcaa ggaagcgggc aatattcacc gcccggaact cggtggcgtt
     9601 attgcgcagc acatccagtt ccggcagttc aatttcagcg gtttcaactt taaggtccaa
     9661 ttcgcgcaat gaagccgccg caagatgcgc ctgaaaatcc atcaggccgc taattcccac
     9721 tacacatatt ttcttcgcgg gcagcggcca gacagggact tctggcgaac ttagccaggt
     9781 tgagcgcaga gtgcctagcg gtgtaatccg ctggtgagcc agctctacgc tgccctgcaa
     9841 ttgcgcaccg ctttcagcga ttaatgcctg cgcctggcaa gcgagatcga gcacgcgttg
     9901 tggcccgaga agagtgtaag gatgggcagg tgcctgctga cgcaaagatt ccagtccact
     9961 gtgaatgtct gtcaccggtt gaccatccgg cagatggctc agcaaatcca gcgatccaga
    10021 tgagaaatgc agcgcgcttt gaccacgagt gacaatggca cagcgcaggc cgtgtttttg
    10081 cagttgcagg ccacagagta atccggcgag gccgccgccc ataatgacag tatcaaagcg
    10141 catctttctg ctccttctcc agaccacaca atccctgata aacccagcgg gtaaattcgc
    10201 tttcgcgcag tgcatctccc caggcgatgg gttgcacgcc tttccagcgt tcgtttagga
    10261 aggtggaaag ttgctcgata gattgcgcgg acgtcgtgac gttaaaacgt tgcagcagtc
    10321 cggcagcgcg gcaggcgcag agctcgccct ggcaggtgcc catccccaca cgggtacgac
    10381 ggcgtaaatc cagcaggcta ttaacgttta aattttctac cgcgtactgc acttcacccg
    10441 cagttaccgc ttcgcactca cataccaggc tacggtgcag acggccttcg ctcagccagg
    10501 caggcgtgcg atcgccatga cgataaaccg cagaaccgcg cagcggggca ggcagggaga
    10561 tgactttacg caaggtaact tcagcgggtt cctgtgaacc aggaagtgcc agatcggcgg
    10621 tcgtacaggg gcgcgtgttt cccagtttgc ggcatacggc gtcggtagcc cattccgcca
    10681 tcagccgata ggtcatcagt ttgccaccgg tgatggtgat aaacccgtcc agaccatcgc
    10741 gttcagcatg gtcgagcagc acgatgccac ggctgacgtt acgtccgctc ggatcgtcat
    10801 cgctggcaac cagcgggcgt acgccagagt aggcccgcaa aatgcgcatt ttcgccatca
    10861 cgggggccag tttttcccct tcgcgcagca gaatatcaac ttcttctgcc gtcactcgat
    10921 tatcgtcaat ctcgttgtaa tcaatacgta aagaggtggt gccaatcagc gaaatggtat
    10981 cgccaggcac cagaatatcg gcgtcggaag gtttgcggca gcggttgatc acatgctgat
    11041 taatgcggtg atccatgatc agcagcgatc ctttcgccgg gaacatgcga atgcgcagat
    11101 cggcatattc ggcaatgtgt tgcccccaga tcccggcggc attaactacg acaggcgcat
    11161 gaagtgtctg aatttcgccg gtgagatggt tacgtacacg aacaccgcac accgtcgcgc
    11221 cttcacgaat gagtcccgtg acttcatgag cagtaaggat aaccgcaccg tgttctttgg
    11281 catccagcat atttgctgtg gtcagacgaa atggatcaac ggtgccatcc ggaactttca
    11341 ccgcgccaat cagtgccggg ttaacggcag gttcgataat gcgcgcttgc tgcggatcaa
    11401 tggcttctgc gctgatccct gcttcttcgc aggcgcggat aaaagtggcc tggaaggaga
    11461 ggtcatcttc cggcagggtg ataaacaggc cgttggttgg ttctacgcag tgacgtgcaa
    11521 tgcgtttcag gatctggttt tcactaatac attcgcgggc cgattccgca tcggttaccg
    11581 catagcgcgc accgctgtgc agcaggccgt ggttacgccc ggtggcaccg gttgcgatgt
    11641 cgtggcgctc aaccaaaatc acgcgcagcc cgcgcagggc acagtcgcgg gcaatcccgg
    11701 ctcccgttgc gccaccgcca atgataatca cgtcacttga ttgcgagtcg cgagttttca
    11761 ttgttatccc tctgaagttc gttttttacc atttagccat agtaaaaaca tgaattgttt
    11821 gatttcgcgc atattcgctc ataattcgaa agtgaaacgt gatttcatgc gtcattctga
    11881 acatttcaac catcgtattt aataatgtgt gcggtaattc acatttaatt tatgaatgtt
    11941 ttcttaacat cgcggcaact caagaaacgg caggttctcc cactgaatca ggctgttaat
    12001 cataaataag accacgggcc acggaggcta tcaatgttga gtatttttaa accagcgcca
    12061 cacaaagcgc gcttacctgc cgcggagatc gatccgactt atcgtcgatt gcgctggcaa
    12121 attttcctgg ggatattctt tggctatgct gcttactatt tggttcgtaa gaactttgcg
    12181 ctcgctatgc cttatctggt tgagcaggga ttctcacgcg gtgatttagg ttttgccctt
    12241 tcggggatct cgattgctta tggattttcg aaattcatca tgggttcggt atcggatcgc
    12301 tcgaatccgc gcgttttcct gcccgcaggt ttgattctgg cggcggcagt gatgttgttt
    12361 atgggctttg tgccatgggc gacgtcgagc attgcggtga tgtttgtact gttgttcctc
    12421 tgcggttggt tccaggggat ggggtggccg ccgtgtggtc gtactatggt gcactggtgg
    12481 tcgcagaaag aacgtggcgg tattgtgtca gtgtggaact gtgcgcacaa cgtcggtggt
    12541 ggtattccgc cgctgctgtt cctgctgggg atggcctggt tcaatgactg gcacgcggcg
    12601 ctctatatgc ccgctttctg tgccattctg gtggcactgt tcgcctttgc gatgatgcgc
    12661 gataccccgc aatcctgtgg cctgccgccg atcgaagagt acaaaaatga ttatccggac
    12721 gactataacg aaaaagcgga acaggagctg accgcgaagc agatcttcat gcagtacgtg
    12781 ctgccgaaca aactgctgtg gtatatcgcc atcgccaacg tgttcgttta tctgctgcgt
    12841 tacggcatcc tcgactggtc accgacttat ctgaaagagg ttaagcattt cgcgctggat
    12901 aaatcctcct gggcctactt tctttatgaa tatgcaggta ttccgggcac cctgctgtgc
    12961 ggctggatgt cggataaagt ctttcgtggt aaccgtgggg caaccggcgt cttctttatg
    13021 acactggtga ccatcgcgac tatcgtttac tggatgaacc cggcaggtaa cccaaccgtc
    13081 gatatgattt gtatgattgt tatcggcttc ctgatctatg gccctgtgat gctgatcggt
    13141 ctacatgcgc tggaactggc accgaaaaaa gcggcaggta cggcagcggg ttttaccggg
    13201 ctgtttggtt acctcggcgg ttcggtggcg gcgagcgcga ttgttggcta caccgtggac
    13261 ttcttcggct gggatggcgg ctttatggta atgattggcg gcagcattct ggcggttatc
    13321 ttgttgattg ttgtgatgat tggcgaaaaa cgtcgccatg aacaattact gcaaaaacgc
    13381 aacggaggct aatggcatga aattgacgct gaaaaacctt agcatggcga tcatgatgag
    13441 cactatagtc atgggaagca gtgcaatggc ggcggacagc aacgaaaaaa tagtcatcgc
    13501 ccatcgcggt gccagtggat atttgccgga gcatacgctg ccagcaaaag cgatggcgta
    13561 tgcgcaggga gcggattatc tggaacagga tttggtgatg accaaagacg accatctggt
    13621 tgttctgcat gaccattatc tcgatcgtgt tactgatgtt gccgatcgtt tcccggatcg
    13681 ggcgcgcaaa gacggtcgtt actacgcgat agatttcacg ctggatgaaa ttaagtcgct
    13741 gaaatttacc gaaggtttcg atattgaaaa cggtaaaaaa gtgcagactt atccggggcg
    13801 tttcccaatg ggtaaatctg acttccgggt gcacaccttt gaagaagaga ttgaatttgt
    13861 tcaggggtta aaccactcta ccgggaaaaa tatcggtatc tatccagaaa ttaaagcgcc
    13921 gtggttccat catcaggaag ggaaggatat tgcggcaaaa acgctggaag tgctgaagaa
    13981 atatggttac accggtaagg acgacaaagt ttatttgcaa tgttttgatg ctgatgagct
    14041 gaagcgtatt aagaatgtgc tggaacccaa aatgggtatg gatctcaatc tggtacagtt
    14101 gattgcctat accgactgga atgaaacgca gcagaaacag ccggacggaa gctgggttaa
    14161 ttacaattac gactggatgt ttaagccggg ggctatgaaa caggtggcgg aatatgctga
    14221 tggtattggt ccggattacc atatgttgat tgaggagaca tcgcagccgg gtaatatcaa
    14281 actcactggc atggtgcaag atgctcagca gaataaactg gtagtgcatc cttataccgt
    14341 gcggtcagat aaactgcctg aatacactac tgatgtgaat cagttatatg atgctctgta
    14401 taacaaagcg ggtgtaaatg ggctatttac tgatttccct gataaggcag taaaattcct
    14461 taataaagag taaattaact accggatggc acttatgtgc tatccgatac cactcttcat
    14521 taaagtctgt gggttatcct taattgcaat tatttagagc gagatgactt gttcccggca
    14581 taagatactc gtcaatacaa cacgtattat taagattttc ctcagctaaa catctatagt
    14641 cattgtcgtt aataaaaaac gacaggcgat agggtatggc agtttcagca aagcacgacg
    14701 aatttaatca ctggtgggca acagagggcg actgggttga agagcctaac tatcgccgta
    14761 atggtatgag cggggtgcaa tgcgtcgagc gcaacggcaa aaagctgtat gtaaagcgca
    14821 tgacgcatca tctgtttcat tccgtacgtt atccgttcgg ccgaccaacg attgtccgtg
    14881 aggttgcggt aattaaagaa ctggaacggg ccggtgtcat cgtgccgaag atcgtttttg
    14941 gtgaagcggt gaaaattgag ggtgaatggc gcgcgttgct ggtgactgaa gatatggcgg
    15001 ggttcatcag cattgctgac tggtatgccc ggcatgcagt atcgccttat tctgacgaag
    15061 tacggcaagc catgttgaaa gcagtggcgc tggcgtttaa gaaaatgcat agcgttaatc
    15121 gtcagcatgg ctgttgttat gttcgccata tctatgtgaa aacagaaggt aaggcagaag
    15181 ctggatttct tgacctggag aaaagtcgtc gccgcttgcg tcgtgataaa gcgattaatc
    15241 atgacttccg ccagctggaa aaatatctgg agccgatccc caaagcggac tgggagcagg
    15301 tgaaagcata ttactacgct atgtaatcgt tgtaggccag ataagacgta taagcgtcgt
    15361 atctggcgtt aaaaccaatt cacatctcga tttcaatatc gccttttgcc cggcaacaac
    15421 agggcaaaat ttcccccggc tgaataaaag ctaatggttc ggcaatccag tccacctggc
    15481 ctgcgaccag tcgggtgcga caggagccgc agtaaccttc gcggcactgg tactcaaccg
    15541 ccacattgtg ggattccagc gccgccagaa gggaagggtg ttcatcctgg cacagcagtt
    15601 gtgtgccagt aatgcgcagg gtaacgcggg ccatcagagc tggaagttac tcaaatcgtc
    15661 ggtgtccact tccgcgtcaa tctgcccgac cagataagaa ctgacttcca cttcctgcgg
    15721 agcaacctgc acgttatcag acaccagcca ggtgttgatc cacgggatcg ggttggagcg
    15781 cgtctggaac ggcagatcca aaccgactgc ctgcatacgg atattggtga tgtattcaac
    15841 gtactggcag agaatgtctt tattcagacc aatcatcgaa ccatcacgga acagataatc
    15901 cgcccagtct ttctcttgct gtgccgcctg gacaaacagg tcatagcact cctgcttaca
    15961 ttcttcggca atttccgcca tctccggatc atccgcgccg ctgcgcagca gattcagcat
    16021 atgctgggtg ccggtcaggt gcagggcttc gtcgcgggca atcaggcgaa taattttggc
    16081 gttaccttcc atcagctcac gttcagcgaa tgcgaaagag caggcaaagc tgacgtagaa
    16141 acggatcgct tccagcgcgt taacgctcat caggcagaga tacagttttt tcttcagctc
    16201 gcgcaggcta acggttacgg ttttaccgtt aacggtgtgg gtgccttcgc ccagcagatg
    16261 ccagtagctg gtcatttcga tcagttcatc gtaatagctg gagatccctt ccgcacgttt
    16321 ctggatctgc tcgttggtga cgatatcgtc aaagacaact gacggatcgt taacgatatt
    16381 acggatgata tgggtatagg agcgggaatg aatcgtttct gagaacgccc aggtttctac
    16441 ccaggtttcc agttccggga tcgagatcag cggcagcagt gccacgttcg gactgcgacc
    16501 ctgaatggaa tccagcagcg tctgatattt caggttgctg ataaagatgt gtttttcatg
    16561 ttcaggcagt gcctggtaat cgatacggtc gcgggagacg tcaacttctt ccggacgcca
    16621 gaagaaagag agctgctttt cgatcagctt ttcgaagatg tcatattttt gctgatcgta
    16681 gcgagccacg ttgaccggct gaccaaagaa cattggttct ttgagctgat catttttcgt
    16741 ctgtgaaaag gtggtatatg ccatgaatgt gtcctgttag gagttttatg ccggatgcgc
    16801 ttcgcttatc cggcctacaa accgcatcgt aggctgggta gggcgttcat gccgcatctg
    16861 ggcatccagt gccggatgcg cttcgcttat ccggcctaca aattggagcc gtaggccggg
    16921 tagggcgctc acgccgcatc cggcatcaca atatcagatc ttacatgcgc cgctttcgca
    16981 gccatcgtcc tggattgacg gcaccagatc gtcctgcgcg tcttctgcgc cgtcacgggt
    17041 gttctgataa tacagcgttt tgaccccgaa tttgtaggcg gtgagcaggt ctttcagcaa
    17101 ctgttgcatc ggcacttttc ctgacgggaa acgtgacgga tcgtagttgg tgttagcaga
    17161 gatcgactga tcgataaatt tctgcatgat acccaccagt tgcaggtagc catcgttacc
    17221 cggcatttcc cacagcagct cataggcgtc gtgcagatgc tcgtagtccg gcaccacttg
    17281 gcgcagaata ccgtctttcg acgctttgat gctgacgtaa ccgcgcggcg gttcaatacc
    17341 gttagtggcg ttagagatct gcgaagaagt ctcggacggc atcagggcag aaagcgtgga
    17401 gttacgcagg ccgtgcgttt tgattgactc acgcagtgct tcccagtcgt aatgcagcgg
    17461 ctcattagcg atggcatcca gatctttctt ataggtatcg atcggcagga tccctttcgc
    17521 gtaagtggtt tcgttaaacc acgggcacgc gccttgctct ttcgccagct cattagacgc
    17581 tttcagcagg taatactgaa tggcttcgaa ggttttatgc gtcaggttgt tggcgctgcc
    17641 gtcggagtag cgtttaccgt gcttcgccag gtagtaagcg aagttgatca caccaatacc
    17701 cagcgtacga cgacccatcg ctccacgttt ggcggccggg atcgggtaat cctgataatc
    17761 cagcagcgcg tcgagtgcgc gtactgccag aattgccagc tcttccagtt catccaggct
    17821 gttaattgcg cccaggttga aagcagacag cgtacacagc gcgatttcac cgttctcgtc
    17881 gttgacgtcg ttcagcggtt tggtcggcag ggcgatttcc aggcacaggt tagactggcg
    17941 cactggcgcg atggccggat caaacgggct atgggtattg cagtggtcaa cgttctgaat
    18001 atagatacga ccggtagacg cacgttcctg catcatcagc gagaacagct caacggcttt
    18061 cacacgctgc ttgcggatgc tgtcgtcttt ctcatatttg gtatacaggc gttcaaactc
    18121 ttcctgatcg gcgaagaacg cgtcgtacag ccccggtacg tcggacgggc tgaacagagt
    18181 gatatcttca cctttcagca ggcgggtata catcagtttg ttgatttgaa ccccgtagtc
    18241 catatgacgc acgcggttgc cttcaacccc acggttgttt ttcaacacca gcaggctttc
    18301 cacttccaga tgccacatcg ggtagaacag cgttgccgca ccgccgcgca cgccgccctg
    18361 agagcaggat ttcaccgcgg tctggaaatg tttgtagaac ggaatacaac cggtatggaa
    18421 cgcttcgcca ccgcgaatcg ggctacccag cgcacgaata cgcccggcgt taatgccgat
    18481 cccggcacgc tgggaaacgt atttaacaat cgcgctggag gtggcgttga tggaatccag
    18541 gctgtcgccg cactcaatca gcacgcagga gctgaactga cgagtcgggg tacgcacgcc
    18601 ggacatgatt ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt aaaaacgctt
    18661 cacgtattgc aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg caaccagaat
    18721 ataaaggaac tgagcgcttt cgtagatttc gccggtcacg cggttctgta ccagatattt
    18781 gccttccagc tgcttaacgg cagcataaga gaaggtcata tcacggtcgt gatcgataaa
    18841 cgtgtccatc tgcttgaact cttcttccgt gtagtcttcc agcagatgat tatcgtattt
    18901 gcccatctcg accattttca ccacatggtc gtacagcgca ggcggctcaa actgaccgta
    18961 ggctttttta cgcaggtgga agatcgccag acgtgcggcg agatactgat aatccggcgc
    19021 atcacgggag atcaggtctg cggcagcctt gataatagtt tcgtggatgt cagaggtctt
    19081 gataccgtca taaaactgaa tgtgggagcg cagctcgacc tgggaaatcg aaacgttatg
    19141 cagtccttct gccgcccaat ccagaacgcg atggattttg tcgagattga tgcgctctgt
    19201 gctaccgtcg cgctttgtca ccagcagatt ctgattcatg tatgtcgtac ctgtttttca
    19261 atagcttcat gcagactacc tgtagtgagg gaagctgcta agaacactat atataggggg
    19321 tatgtttggg attcactgca agatagtgtg aaactggccc tcttgcaagt gcataacttt
    19381 gtggataact caggaaggaa aaagtggctt ccgcgcacct taggtcagac aaggtgtccg
    19441 ggaaagtcaa tgggaagaga aaaatttgtt aaaaataacc gttcggtgaa ttcttgaacg
    19501 gtaaaatagg ataggtaaat tgatatagat ggtttattaa tcttgttgct aatgattgcc
    19561 aaaattgatc taggcacaag tcgtcacata atctgatgta tcagttgtgg tcaatatata
    19621 agcgagtgta agcgattgtt gagtcggaaa tggttgtttt atat
//
LOCUS       DADTFG010000090        19646 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.090, whole genome shotgun sequence.
ACCESSION   DADTFG010000090 DADTFG010000000
VERSION     DADTFG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.090"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            44..595
                     /locus_tag="J1138_002968"
     CDS             44..595
                     /locus_tag="J1138_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBA8454737.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQI
                     PTGDLDTAVKYGERVATITARLK"
     gene            complement(773..1765)
                     /gene="rpoS"
                     /locus_tag="J1138_002969"
     CDS             complement(773..1765)
                     /gene="rpoS"
                     /locus_tag="J1138_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBA8454738.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1828..2967)
                     /gene="nlpD"
                     /locus_tag="J1138_002970"
     CDS             complement(1828..2967)
                     /gene="nlpD"
                     /locus_tag="J1138_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBA8454739.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSSGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3107..3733)
                     /gene="pcm"
                     /locus_tag="J1138_002971"
     CDS             complement(3107..3733)
                     /gene="pcm"
                     /locus_tag="J1138_002971"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBA8454740.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3727..4488)
                     /gene="surE"
                     /locus_tag="J1138_002972"
     CDS             complement(3727..4488)
                     /gene="surE"
                     /locus_tag="J1138_002972"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBA8454741.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4469..5518)
                     /gene="truD"
                     /locus_tag="J1138_002973"
     CDS             complement(4469..5518)
                     /gene="truD"
                     /locus_tag="J1138_002973"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBA8454742.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5515..5994)
                     /gene="ispF"
                     /locus_tag="J1138_002974"
     CDS             complement(5515..5994)
                     /gene="ispF"
                     /locus_tag="J1138_002974"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBA8454743.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5994..6704)
                     /gene="ispD"
                     /locus_tag="J1138_002975"
     CDS             complement(5994..6704)
                     /gene="ispD"
                     /locus_tag="J1138_002975"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBA8454744.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6723..7034)
                     /gene="ftsB"
                     /locus_tag="J1138_002976"
     CDS             complement(6723..7034)
                     /gene="ftsB"
                     /locus_tag="J1138_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBA8454745.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7227..7550)
                     /locus_tag="J1138_002977"
     CDS             complement(7227..7550)
                     /locus_tag="J1138_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBA8454746.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7600..8205)
                     /gene="cysC"
                     /locus_tag="J1138_002978"
     CDS             complement(7600..8205)
                     /gene="cysC"
                     /locus_tag="J1138_002978"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBA8454747.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8205..9632)
                     /gene="cysN"
                     /locus_tag="J1138_002979"
     CDS             complement(8205..9632)
                     /gene="cysN"
                     /locus_tag="J1138_002979"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBA8454748.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIHYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9634..10542)
                     /gene="cysD"
                     /locus_tag="J1138_002980"
     CDS             complement(9634..10542)
                     /gene="cysD"
                     /locus_tag="J1138_002980"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBA8454749.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10794..11831
                     /gene="iap"
                     /locus_tag="J1138_002981"
     CDS             10794..11831
                     /gene="iap"
                     /locus_tag="J1138_002981"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBA8454750.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     NTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11956..12108)
                     /locus_tag="J1138_002982"
     CDS             complement(11956..12108)
                     /locus_tag="J1138_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA8454751.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12372..13106)
                     /gene="cysH"
                     /locus_tag="J1138_002983"
     CDS             complement(12372..13106)
                     /gene="cysH"
                     /locus_tag="J1138_002983"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBA8454752.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SDSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13180..14892)
                     /gene="cysI"
                     /locus_tag="J1138_002984"
     CDS             complement(13180..14892)
                     /gene="cysI"
                     /locus_tag="J1138_002984"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBA8454753.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14892..16691)
                     /gene="cysJ"
                     /locus_tag="J1138_002985"
     CDS             complement(14892..16691)
                     /gene="cysJ"
                     /locus_tag="J1138_002985"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBA8454754.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLAASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            17007..17372
                     /gene="queD"
                     /locus_tag="J1138_002986"
     CDS             17007..17372
                     /gene="queD"
                     /locus_tag="J1138_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBA8454755.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            17450..18721
                     /locus_tag="J1138_002987"
     CDS             17450..18721
                     /locus_tag="J1138_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA8454756.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILHARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18712..18972
                     /locus_tag="J1138_002988"
     CDS             18712..18972
                     /locus_tag="J1138_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBA8454757.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18989..19564
                     /locus_tag="J1138_002989"
     CDS             18989..19564
                     /locus_tag="J1138_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBA8454758.1"
                     /translation="MSLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVTQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
BASE COUNT         4557 a         5320 c         4963 g         4806 t
ORIGIN      
        1 aacctgcctc aaaaaatcat tcattagtaa aaaggatatt tatatgcaaa acatagctgt
       61 ggtttattat tcaggatatg gtcacactaa actggttgct gaaaaagtgg cacaaggcgc
      121 ccatgcagat ttaatttcga ttgataacga aggaaatatc actgagcagg agtgggaaaa
      181 actggacgca gccgatgcca ttatcttcgg tgctcccacc tatatgggcg gtgcgccatg
      241 gcaattcaag aaatttgctg atgccagttc caaagcgtgg tttacacgta aatggcagga
      301 taaagtcttc gggggtttca ccaacagcgc cagcctgaat ggcgataaac aagttacgct
      361 gatctatctg caaacgctgg cttcacagca tggcggtctg tgggtgagtt taggtcaggc
      421 tcctgccaat gtgcttgcgt caaccaggga agatgtaaac aatctgggcg gttcagtcgg
      481 tttgctggtg caatctccag ccgatgcggg tgcagaccaa atccctacgg gcgatctgga
      541 tacagccgtg aaatatggtg aacgcgtggc gacaattact gcgcgcttaa aatgatgatt
      601 gccgaatgtg acgctgacgc gtctatccgg tcttctacat ctaaaaagtg ccgtcccctg
      661 gtcagtcaga taaagtttta acgctgtatc cgataacagt tcaacatgta ccatgtacca
      721 aaagaaaaaa ggccagcctc gcttgagact ggcctttctg acagatgctt acttactcgc
      781 ggaacagcgc ttcgatattc agcccctgcg tttgcaggat ttcgcgcaaa cggcgcaggc
      841 cttcaacctg aatctggcga acacgttcac gggtgaggcc aatttcacga cctacatctt
      901 ccagtgttgc cgcttcgtac cccagcaaac cgaatcgacg tgccagcact tcacgctgtt
      961 tggcgttcag ctcgaacagc catttgacga tgctctgctt catatcgtca tcttgcgtgg
     1021 tatcttccgg accattctct ttttcatcgg ccaggatgtc cagcaacgct ttttcggaat
     1081 caccacccag cggggtgtct accgaggtaa tgcgctcgtt aagacgaagc atacggctga
     1141 cgtcatcaac tggcttatcc agttgctctg cgatctcttc cgcacttggt tcgtggtcca
     1201 gcttatggga caactcacgt gcggttcgca ggtaaacgtt cagctccttt acgatgtgaa
     1261 tcggcaaacg aatagtacgg gtttggttca taatcgcccg ttcgatcgtc tggcgaatcc
     1321 accaggttgc gtatgttgag aagcggaaac cacgttccgg atcaaacttc tctaccgcgc
     1381 ggatcagccc caggttgccc tcttcgatca ggtccagcaa cgccaggcca cgattgccat
     1441 aacggcgggc aatttttacc accagacgca agttactctc gatcatccgg cggcgagagg
     1501 cgacatctcc acgcagtgcg cgacgcgcaa aataaacttc ttcttcggcc gttaacagtg
     1561 gtgaataacc aatctcacca aggtaaagct gagtcgcgtc cagcacacgc tgtgtggctc
     1621 cctgcgataa cagttcctct tcggccaaat cgttatcact gggttcctct tctactaagg
     1681 ccttttcgtc aaaaacctca actccgttct catcaaattc cgcatcttca tttaaatcat
     1741 gaactttcag cgtattctga ctcataaggt ggctcctacc cgtgatccct tgacggaaca
     1801 ttcaagcaaa agcctggttc cgccgattta tcgctgcggc aaataacgca gcgggtttac
     1861 ggatttcccc ttgtaacgaa tttcaaaatg caagcgtgtt gaactggttc cggagctacc
     1921 catggttgct attttttgcc ccgccttcac ttcttgttgt tcccggacca gcattgtgtc
     1981 gttatgggcg taggcactca ggtaatcatc attatgtttg atgataatca gattaccgta
     2041 gccgcgcagc gcgttaccgg cataaacaac gcggccatcc gcggtcgcga taattgcctg
     2101 tcctttgctg cctgcgatat caatcccctt gttgcccccc tcagaagcgc caaaggtttc
     2161 gatcactttg ccctcagtcg gccagcgcca ggtggagata ggcgtactgg ttgatgtact
     2221 gctgacagtc ggctcggttg tgcttgctgt tggtaccgtt acaggcgctg tgaccgtggt
     2281 cgcagttggc ttgttgttcg gcaacatttt gttagcactc tgttcacccg aagactcaga
     2341 atacgtaatt gtcggttgcg acgcaacagc aacggtggaa ttttgtgcag gcttgatcac
     2401 aactccttgc tctgctgcgt cggcctgggt aatggcattt ccgcctgtga ttggcgtacc
     2461 ggaagcatta cccacctgta aggtctgacc gacgttcagc gcgtatggtg cctgaatatt
     2521 gttgcgctga gcaaggtcac ggaaatcgtt gccagtaatc caggcgatat aaaaaagcgt
     2581 gtcgcctttt ttcacggtgt atgtactgcc gctataactg cctttcggaa tgttcccata
     2641 ctgacggttg tagacgattc gtccgttttc catctgtacc ggctgctgag ctactggctg
     2701 cactggctgg atttgcggtt gttgagtagc ctgaatttgt ggctgctgta ccggctgaat
     2761 ttgcggttgc tgcgctgtag acgtcgtccc cattttcggc ggcggcgtaa tcaacatacc
     2821 agaattggta tttgcaggcg cattgccatt aacggagctg accggtgccg gtggatttga
     2881 agtgtcagaa cagcctgcca gccatagcga aaccagtgac aatgccgcaa tgcggcgaac
     2941 ggtgaatttt gggcttcccg cgctcattta tcccccagga aaaattggtt aataaccagt
     3001 gacataatta ccgtgcaagg caccatactg aacactggaa aagatgttca cgatacgctg
     3061 acttgcggca aaataaccag gaaaaatcca ggtatttcct cacgttttaa gccagctcac
     3121 ccttcactaa agggacaaag cgcacggcct ccacggtatc gataataaat tcgcctcccc
     3181 gacgacgcac ccgtttcaaa tactggtgct cctcccctac gggtaagacg agaatcccgc
     3241 cttcgtccag ctgcgtcatt agcgcagttg gaatttcagg cggtgccgcc gtaacaatga
     3301 tagcgtcaaa cggcgcgcgt gcctgccaac cttgccatcc atcgccatga cgggttgaaa
     3361 cattatgtaa atcaagattt ttcaggcggc gacgcgcctg ccactgcaag cctttaatcc
     3421 gttcaaccga gcaaacatgt tggacaagat gcgccaggat tgcagtttga tatcccgaac
     3481 cagtgccaat ttccagcacc cgcgactgcg gcgtcagctc gagtaattcg gtcattcgcg
     3541 ccaccatata cggctgcgaa attgtctgcc cctgacctat cggcaaggcg atattgtccc
     3601 aggctttttg ttcaaacgct tcatcaacga atttttcacg cggcacggcg gcaagtgcat
     3661 tcagcacctg ctcatcctga ataccttgcg cacgtaattg atccagaagt gcttgtacgc
     3721 gtctgcttac cattgcgtgc caactcccac gctgtttaac cagtctgaaa ccacatcttg
     3781 cgcgctatgc gcagttaaat ccacatgcag cggcgtgatg gaaacatagc cctcatctac
     3841 cgcagcaaaa tcggtccccg gaccggcatc acatttaccg cccggcgggc caatccagta
     3901 cagcgtattg ccgcgcggat cttgctgcgg gatcacctga tctgccggat gtcgtgtacc
     3961 gcagcgcgtc acgcgaatac ctttgatttg atccaatggt aaatccggaa cgttaatatt
     4021 aagaatacgc ccggtgcgca gcggctcttt acacagtgcg cgcaaaattg agcaggttac
     4081 cgccgcggca gtgtcgtaat gtttatgccc gtcaagcgag acggcaagcg ccggaaaacc
     4141 taaatggcgg ccttccatcg cggccgctac cgtaccggaa taaataacat catcccccag
     4201 attcggcccg gcgttaattc cggacacaac aatgtccggg cgcgggcgca tcagagcatt
     4261 tacgccaaga tagacgcaat cggtcggggt tcccatttgc acagcaatat caccattttc
     4321 aaaggtaaac gtgcgcaggg aggattccag tgtcagagaa ttagaagcgc cgctgcggtt
     4381 acgatcgggg gcgaccacct gaacgtcagc aaactcacgc aaggctttcg ccagcgtttg
     4441 tataccgggt gcatgtaccc cgtcatcatt actcagcaat atgcgcataa tcacctgttg
     4501 tgttgataag ttccctgaca acgctggttg caaaacttcc cgccggaagc cagaaacgga
     4561 tttctacggt gacgtcatcc caccagttcc agcttaattg ttgcggatac agcagcatcg
     4621 ctctgcgcgc ggcttcaact ttttcgcgca ccagtaaggt ttgtaattca gtttcttcgg
     4681 cgacagctgc ttgttcgaat gccagcgctt cacgctgagt tccccattcg ccactgcccg
     4741 gcaatgcggc ggttatcatc agctctttat cgttgacgcg acgctgtaat tccgccagtt
     4801 cttcggtggt tgcgacaaac cagctaccac gtccggctaa ttgtagcgca tcgccgtcaa
     4861 caacttgatt aacgtctgct tttttgaggc gctcagcaac aatctgatta aacaacgcac
     4921 tgcgggctgc cgacaaccaa aaactccgtt tattgcgatc gcgcaccgga gtattggttt
     4981 gcgcccagcg cagcgcaccc tgcaagttgc taccgccaat cccaaaacgt tgggcaccga
     5041 agtagttcgg tacacctttt acgcaaatat cgatcagacg ttgttcaacg tcatcgcgat
     5101 tgctcacttc gcgtagtacc agggtaaagg cgttaccttt cagcgcccct aaacgcagct
     5161 tgcgcttgtg ccgcgcatac tccagcacct ggcagccttc cagttgaaag gcgctcagat
     5221 cgggcatctc tttgcccggc acgcgagcgc ataaccactg ttccgtaaca gcatgtttat
     5281 ctttttgccc agcgaagctg acttcacggg catgaatttt caggaatttc gccagtgcat
     5341 ccgccacaaa acgggtattg cagccgtttt tgaggattct aaccagaata tgctcacctt
     5401 caccatcagg ctcaaagccc aaatcttcca ccaccacaaa gtcttccgga ttggctttca
     5461 gcagcccggt gccttgcggt ttaccgtgga ggtaagtgag attatcaaac tcaatcattt
     5521 tgttgcctta atgagtagcg ccaccgcttc acaggcaatc ccttccccac gtccggtaaa
     5581 tcccagtttt tccgtagtag tggctttcac gttaacatca tccatatggc agccgagatc
     5641 ttcggcaata aacacgcgca tttgtggaat gtgcggcaac atcttcggtg cctgagcgat
     5701 gatagtgaca tcgacgttgc cgagggcata acctttcgcc tgaatacgac gccaggcttc
     5761 gcgcaacagc tcgcggctgt cggcaccttt aaatgccgga tcggtatccg ggaacagctt
     5821 gcctatatct cccagcgccg ccgcgccaag caatgcatcg gtcaacgcat ggagcgccac
     5881 gtcgccatca gaatgcgcca gcagcccttt ttcgtaagga atgcgtacgc caccaatgat
     5941 aattgggcct tcaccgccaa aggcgtgtac gtcaaaaccg tgtccaattc gcattatgta
     6001 ttctcctgat ggatggttcg ggtgaggtaa aattcggcca gcgccaaatc ctccgggcgc
     6061 gttaccttga tgttatccgc gcggccttca acaagttgtg gatgaaaacc gcaatattcc
     6121 agcgccgagg cctcatcggt aatggtagcg ccttcattta gagcgcgcgt caggcagtca
     6181 tgtaacagct cacgagggaa aaattgcggc gtcagcgcgt gccataagcc gttgcgatca
     6241 acggtatgag caatggcatt tttgcccggt tcggcacgtt tcatcgtatc gcgcaccggc
     6301 gcagcgagga tccctcccgt gcggctggtt tcgctcaacg ccaacaatcg cgcgaggtca
     6361 tcctgatgca gacaaggacg agcggcgtca tgcaccaata cccactgcgc gtcgccagcg
     6421 gctttcagac ctgccagcac ggaatcggca cgctcttcac caccatcaac aaccgtgatt
     6481 cgtggatgat tcgccagagg aagttgtgca aagcggctat cgccaggact tatggcaatg
     6541 acgacacgtt tcaccctggg atgcgccagc agcgcatgca ccgagtgttc aagaatggtt
     6601 tgattaccga ttgagagata ttgcttagga cattccgttt gcattcggcg gccaaatccg
     6661 gccgccggaa ccacggcgca aacatccaaa tgagtggttg ccatgttaat tcccgggctg
     6721 atttatcgat tattttgccc cgcagactgt gcgcgctttg acgcgtcagg caccagacga
     6781 taaaacgttt cgcccggcct ggtcatgctg agttcattac gcgcacgctc ttcgagcgcc
     6841 tcctggccgc cattgagatc gtcaatttcg gcaaaaagct gatcgtttcg cgctttaagt
     6901 ttcgcgtttg tagcttgctg tgccgccaca tcattattga cgcgagtata gtcatgtata
     6961 ccgttcttac cgaaccacag cgaatactgt agccagacca gaatagccag caacagcagc
     7021 gttagtttac ccatcctgcc ccctgaaaaa cggcatcttc atcccatgca tccgaagacg
     7081 actctacatc ctctgttggg gataccgcga caacgcgggc aaatgtacca catttgtcca
     7141 ttgttacgta tacccagggc gtgcagaaca taatctcatt attagttacg gattgaatta
     7201 tgaacagaat agagaggaaa gaacaattaa cccagtagcc acataaacag cgcaccaaac
     7261 ataatgccaa ccgtcgccag agtgaaaaca atactgtagc gtagctttcc gtccatcaat
     7321 gaatgcagtg caatccccac cactacagcg acgggcatca gcgccagaaa gaaaggccag
     7381 gtgtagataa aaaagagcag cgtgttagag ccataaatca acatcggaat tgccaacgcg
     7441 cacagccacg ataaaaaacc aaccacagcg cctggcagcg accatgtggt gtcttcaacc
     7501 tcagtaaggc tgtcgttatt tgttaatgta atgttgtggc tgttacgcat atctgatcct
     7561 gttactttga cgaaccgggc atggaaaccc ggcggtgtct caggatctga taatatcgtt
     7621 ctgtctcaac agatctaata attgctgtac caaatttgtt actaattgtt caccattgag
     7681 atgaatttct gccgattcag gcgcttcgta aacggaatct attcccgtaa agttgcgcag
     7741 ttcaccggca cgcgctttct tatataaacc ttttggatcg cgggcttcgc aaatcgccag
     7801 cggcgtatcg acaaacactt cgataaagcg cccttctcct acgcgttcgc gaaccatctg
     7861 gcgttcggcg cggtgtggcg agataaatgc ggtcagcacc accagtccgg cttcaaccat
     7921 caaattcgcc acttcaccga cgcgacggat attctcttta cggtcggcat cgctaaaacc
     7981 gagatcgctg cataatccgt ggcgaacgtt gtcgccatcc agcagatacg tactgacgcc
     8041 aagtttatgt aacgcctcct ccagcgcccc cgcgacggtt gatttaccgg aaccagagag
     8101 gccggtaaac cacagcacaa caccacgatg accgtggtgc tgttcgcgtt gttgcggagt
     8161 tactgggtgg ctatgccaga cgacgttttc gtcatgcaga gccattattt atctcccagt
     8221 aaatcgcgcg caccccaatg cgggaaatgg cggcgaacca gggcattcaa ttccagttcg
     8281 aatgcactga attcagatgg cgcagcagtt gcctggctaa ctggttcgtg caccataccg
     8341 gcacctacgg tcacattact caggcgatcg ataaaaatca gcccaccggt aaccgggttt
     8401 tgctgataac gatctaacac cagtggctcg tcaaaggtga gatctaccag gccgatgccg
     8461 ttcagcggca ggttttcaac ttcgcgttgg gtaaggttat tgatatcaac ctgataatga
     8521 atgccatcaa cacgagcacg cgtcttctta ccggcaattt tgatgtcgta actctggccc
     8581 ggggaaagcg gctgttccgc catccatacc acatccaccg acgcgctctg cacagccggt
     8641 aacgcttcgt ctgccgccag cagcagatcg ccacggctga tgtcgatctc atccgtcagc
     8701 accagggtga tcgcttctcc ggcaaaggct tcttcgcgat cgccatcaaa ggtcacgatc
     8761 cgcgcgacgt ttgattccac accagagggc agcactttga cacgttgccc gacttccacg
     8821 cgaccggatg ccagcgttcc ggcgtagcca cgaaaatcga gattcggacg gttaacgtac
     8881 tgcaccggga agcgcattgg ctgagcatcc accactcgct ggatctccac ggtttccagc
     8941 acttccagca gtgtcggacc gctgtaccac ggcatacttt cactttgcga agccacgttg
     9001 tcgccttcca gtgcggagag cggcacaaag cggatatcca gattacccgg cagctgcccg
     9061 gcaaaagtca gataatcttc acgaatacgg gtgaacgtct cttcactgta atccaccaga
     9121 tccattttgt tgatcgccac gaccagatgt ttgatcccca acagtgtgga gataaaacta
     9181 tgacgacggg tttgatcgag cacgccttta cgggcatcga tcagtaagat cgccagttca
     9241 catgtcgatg cgccagtcgc catattgcgg gtgtactgct cgtgccctgg ggtgtcggcg
     9301 ataataaatt tacgcttctc ggtagagaaa tagcggtagg ccacatcaat agtgatgccc
     9361 tgttcgcgct cagcttgcag gccgtccacc agcagtgcca gatccagctt ttcgccctgg
     9421 gtgccgtgac gcttactgtc gttatgcagc gatgagagct gatcttcgta gatttggcgg
     9481 gtatcgtgca gcaggcgacc aatcagggta cttttgccgt catcgacgct accacaggtc
     9541 agaaaacgca gcaggctttt atgttgttgc gcaatcatcc atgcttcgac gccgccttca
     9601 ttggcgattt gttgtgcaag tgcggtgttc atcttaaaaa tacccctgac gttttttcag
     9661 ctccatagac cccgcctggt cgcggtcaat tacgcggccc tgacgttcac tggtcgtgga
     9721 aaccagcatc tcttcgatga tctccggcag tgtttgcgca tttgactcca ccgcgccggt
     9781 cagcggccaa cagcccagtg tgcggaaacg caccatccgt tttttaatca cttcgcccgg
     9841 ttgcaggtcg atacggttgt catcaatcat catcaacata ccgtcgcgtt ccagaaccgg
     9901 acgttccgca gcaagataca gcggaacaat gtcgatattt tccagccaga tgtattgcca
     9961 gatatcctgc tcggtccagt tagagagcgg gaagacgcgg atgctttcgc ctttgttaat
    10021 ttgcccgttg tagttgtgcc acagctccgg gcgctgattt ttcggatccc agcgatggaa
    10081 gcggtcacgg aaagagtaga ttcgctcttt agcgcgggat ttctcttcgt cacggcgcgc
    10141 accaccgaag gcggcatcaa aaccgtattt gttcagcgcc tgtttcaggc cttcagtttt
    10201 cataatatcg gtatgtttcg cgctgccgtg tacgaatgga ttaatcccca tcgccacgcc
    10261 ttccgggttt ttatgcacca gcagctcgca gccgtaggct ttcgccgtac gatcgcggaa
    10321 ctcatacatc tcgcggaatt tccagccggt atcgacatgc agcaacggga aaggcagcgt
    10381 acctggataa aacgccttgc gcgccagatg cagcatgacg ctggaatctt taccgataga
    10441 gtagagcatc accggatttg agaattctgc agccacctcg cgaataatgt ggatgctttc
    10501 cgcctccagt tgccgcaggt gggtaagtcg tttttgatcc ataaccgttc ctttgcaata
    10561 ccgctatttt cttgccatca gatgtttcga ctatagggag cgtaggagaa cgaatgaaat
    10621 taccaattag aatgagtagt tccttaacgg aataacgatt tggcaaagct aatatcaaaa
    10681 agtgcttaag gcaccggatt tcgggcgttt aggaagattt gaaattgttt tagcgcagca
    10741 gcagtttcat actatggcgg taaaaaaatt tgcatggtat ttaaggactc actatgtttt
    10801 ccgcattgcg ccaccgtacc gctgccctgg cgctcggcgt atgctttatt ctccccgtac
    10861 acgcctcgtc acctaaacct ggcgattttg ctaataccca ggcacgacat attgctactt
    10921 tctttccggg acgcatgacc ggaactcctg cagaaatgtt atctgccgat tatattcgcc
    10981 aacagtttca gcaaatgggt tatcgcagtg atattcggac atttaatagt cggtatattt
    11041 ataccgcccg cgataatcgc aagagctggc ataacgtgac gggaagtacg gtgattgccg
    11101 ctcatgaagg caaagcgccg cagcagatca tcattatggc gcatctggat acttacgccc
    11161 cgctgagcga tgctgacgcc gatgccaatc tcggcgggct aacgttacaa ggaatggatg
    11221 ataacgccgc aggtttaggt gtcatgctgg aactggctga acgcctgaaa aatacgccta
    11281 ccgagtatgg tattcgattt gtggcgacca gcggcgaaga ggaaggaaaa ttaggcgctg
    11341 agaatttact caagcggatg agtgacaccg aaaagaaaaa tacgctgctg gtgattaatc
    11401 tcgataactt aattgttggc gataagctgt atttcaacag tggcgtaaac acacctgaag
    11461 cagtaaggaa attaacgcgc gaccgggcgc tggcaattgc ccgcagccat ggaattgccg
    11521 ccaccacaaa tccaggtttg aataaaaact atccgaaagg caccgggtgt tgtaatgacg
    11581 cagaaatatt cgacaaagcc ggtattgctg tactttcggt ggaagcgact aactggaatc
    11641 ttggcaataa ggatggttat cagcaacgcg caaaaacagc cgcattccct gcaggaaata
    11701 gctggcatga cgtaagactg gataatcaac agcatattga taaggcactt cctggaagaa
    11761 tagaacgccg ctgccgtgat gttatgcgga taatgctacc gttggtgaag gagctggcga
    11821 aggcgtcttg atgggtttga aatgattact cccttgcctg caggaaatca atgccaccag
    11881 aaacagagta taaaaaaggg tgcaggagac caaacaatca caattcagta cgcggggttt
    11941 taccccgcgt ggtggctact tcttcgattc gtaagcgaga actgccttaa actccatacc
    12001 acgttctctg atactcaact cacacaacga gtcgcctacc atcaacgtga atcccagtac
    12061 tgtacaacac agtacgatga ttgccacaag ggcatatttt gtcagcatgt cttgcctccc
    12121 tgcaaagaag agactaacat ccgaattgct acgttcgaac gcgagcctca ggttgataga
    12181 aatatcgcct ggggcttttg tccatctgga acctcgcgaa tgcttaacgc cagacagcct
    12241 caagcaccca atgccattct atacctgtta attctccctg cgacgttctc gttttaatgc
    12301 cggaatgctg ctcgcaaaat aaaaacgccc ccatcatttc tgacagaggc gtttaattta
    12361 tccggcaata tttacccttc gtgtaatcca cattcccttt ttaagccaaa gaaacgcgtt
    12421 tcttcttccg ccatgccggg ttcccattta cgggttgtat gggtatcgcc caccgataaa
    12481 tatccttcat cccataatgg gtgatatttc aggccatgtt tttgcaggta ctgataaata
    12541 gttcggttat cccagtcgat aatcggcagc actttaaata cgccacgctg aattgccagc
    12601 accggtaaat tggcgcggct gtcggattgt tcacggcgca ggccagcaaa ccaggtttgc
    12661 gcattcagtt ctttcagcgc ccggttcatc ggttcgactt tgttgatgtc attgtacttt
    12721 tcaatgcctt caacgccctg ctcccacagt ttgccgtagc gtgcttcctg ccaggcggcg
    12781 ctttcggtag cacggtacac tttcaggttg agcttgagtt tgtccgttaa ctcgtcaata
    12841 aagcggtagg tttccgggaa cagatagcca gtatcggtga ggatcactgg aatatccggg
    12901 cgaatctgat tcaccagatg cagactcacc gccgcctgaa tgccaaagct ggaagaaagt
    12961 acatattcac cgggcagatt atccagcgcc caggctacgc ggccttcagc gtccagtttt
    13021 tccagttggg cgttagtttc cgccagcgcc agaatccggt ctacctttgg cagttcgttc
    13081 agggcgttta gatcgagttt ggacataagt tcctcactgt ttgccttgcc tgatgcaacg
    13141 cttacacatc ttatcaggcc tacaagaccg ggctaatggt taatcccaca aatcgcgcgc
    13201 cggatcgagc accgggcgaa tgatgcccgc acgcaccgta aagtcgccga agccttcacc
    13261 cgcttcgcgc tctttcgccc agcgccctat cagttcatca agcgacgcca ggatttccgg
    13321 ctcggtgatg ttttctttat acatccgtgg gatacgtgtc ccaatgcggt tgccgccaag
    13381 atgcaggttg tagcgccccg gcgctttacc caccaggccc acttccgcca gcatcgcgcg
    13441 accacaaccg ttcgggcagc ctgttacacg catcacgata tgctcatcgc tgacaccatg
    13501 tttcgccatt aaattatcga tgttgtcgat aaaagacggc aggaaacgct ccgcttccgc
    13561 catcgccagc gggcaagtcg ggaatgacac gcaggccatt gagttttcac gctgcggcgt
    13621 gacggcattc attaacccgc tttctttggc gatcttctcg atcttcgctt tctcgctttc
    13681 cggcacgccg gcgatgatca gattctggtt cgccgtaatg cggaaatcac ctttgtggat
    13741 ctttgcgatc tccagcaagc cggttttcag cggccgcccc ggataatcaa gaattcgacc
    13801 attttcgata aacagcgtca ggtgccagtt atcatcaatg cccttaaccc agccaatacg
    13861 atcgccgcgt ccggtaaact catacgggcg aatcggctcg aatttgatcc ccgcgcgacg
    13921 ttcaacttcc gctttaaatg tctcaacccc cacccgctcc agcgtgtatt tggttttggc
    13981 atttttacga tcggttcggt taccccagtc acgctgagtt gtcacgacgg cttccgccac
    14041 cgccagcgta tgctccagcg gcagatagcc aaactcactc gccgtgcggg cgtaggtttt
    14101 cttgttgccg tgttcaatgg aaagcccacc gcccaccagc aggttaaagc ccaccagctt
    14161 gccgttttcg gcgatcgcca cgaagttcat gtcgttggcg tgcagatcga tatcgttctg
    14221 cggcgggatc actaccgtgg ttttgaactt acgcggcagg taggtctgac caaggatcgg
    14281 ctcttcatca gtagtggcga ctttttcctg atcgagccag atctccgcat acgcgcgggt
    14341 acgaggcagc agatgttcag aaattttctt cgcccattca tatgcttccg catgcagctg
    14401 cgactcgtaa gggttcgagg tgcagagtac gttacggttc atgtcgttgg cggtcgccag
    14461 cgcatcaaga ccgactgagt gcagcatctg atgcaccggt ttgacgttct ttttcagaat
    14521 gccgtggaac tggaatgtct ggcggttggt caggcgaatg ctgccataga tggtgttttc
    14581 accggcaaat ttgtcgatcg cctgccactg tttagtggta atcaccccac ccggcagacg
    14641 acagcgaagc agcatcgcgt ggcgcggctc cagcttctgt tcagcacgtt cggcgcgaat
    14701 gtcgcggtca tcctgctgat acatgccgtg gaagcggatc agcaggaagt tatcgccctt
    14761 aaaaccgcct gtcagaccgt cgtttaaatc ttccgcaatg gtgccgcgca ggtagttgct
    14821 ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg accactaaag gccctggatg
    14881 tttttcgctc attagtagac atctcgctga taacggcgct ctacgcgcag ctcacttaaa
    14941 aattcatccg ccgcttcggt gtccatgcca ccaaattcgg caatcacttc cagaagtgcc
    15001 tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga cataaatgtg ggcaccgtca
    15061 ttgatccagc gccacagctc tgcgccctgt tcgcgcagtt tgtcttgtac gtaaattttt
    15121 tctttttgat cgcgcgacca ggcgagatcg atgcgcgtca gcacgccttc tttgacgtaa
    15181 cgctgccatt ccacctggta gaggaaatct tccgtaaagt gtgggttgcc aaagaatagc
    15241 cagtttttac ccggcgcttc gtcggcagca cgctgctgca taaaggcacg gaacggcgcg
    15301 atgccggtgc cagggccaat catgatcacc ggggtttccg gattggttgg caggcggaag
    15361 ttgtcgttat gttcgataaa gacgcggact tcaccctctt cttccacacg atctgcgagg
    15421 aagctggagg caccaccggc acgggcacgg ccttccacgt cgtaacgcac cacaccaacg
    15481 gtaacgtgca cttcgttctc gacttccgcc tgcgaggagg cgatggaata caggcgcggg
    15541 gtcagcgggc gcagcagatt aatcagcgct tcggcatcca gttgcgccgg agagaaacgc
    15601 accatgtcga caatcggcgt cgtcgcggcg taatgctgta atttcgcttt atcgcccacc
    15661 agcggcagca gcgtttcgct gcgggtaagc gtggcatagt tctcaacaat gttggcggtg
    15721 ttgacggtca gttcgaagtg ccactgtagc gcttcgttca gaggcaacgt tttgccctcg
    15781 acggtgacag gttcatcgcc tttcagccac agcagttcga caagttcttt caccagtgcc
    15841 ggatcgttct gataccagac gcccagcgcg tcacccggct ggtaacgcag gccagagtca
    15901 cctaagtcaa tttcaatgtg acgaacgtct ttttcagagt tacgcccggt aattttctgg
    15961 ttaaccgaaa gactggccgc cagcggcgcg tctttgctat acgggctggt gtggatttca
    16021 tttaccgcgc cagtagcgac ggattgcgaa ggcgccgcga caggcgcacg cgatttaagc
    16081 gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct ggtattcaac atcggcatcg
    16141 acacggtcta gcaggcgttc accgcccagt tccgccagct tactgtcaaa atctttcccg
    16201 gactggcaga aaaattcata agagctatcg ccgaggctaa acacggcaaa cgcggtgttt
    16261 tccagctttg gcgctttttt ggagaacagg aacttatgca gcgcgacggc ttcttccggc
    16321 ggttcccctt ccccttgcgt tgacgtcacc acgataagca gtttttcgct ggcgatttgt
    16381 ttgaatttat agtcgcccgc gttcaccagc ttaacgttca gttttgccgc taataaatca
    16441 tcgcgtaatg cttcagcaac ccgacgcgca ttgccagttt gcgaggcgga gataatagtt
    16501 atacccggca tttctgcggc gggcgctggc gtcgctgcaa gcgcagcagg ctgctggttg
    16561 agcacgcccc agaaatagcc agaaacccag gcaagctggg taggagttaa atcggtcgtg
    16621 gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa gcaacgcgga aggtgggacc
    16681 tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc ctgtcagcgc
    16741 ccataaaaca gaagagaagg taaggttaac ggggtgaacg gtgtggatta aagacgggat
    16801 agcgataact aataaccaaa tcgactaacc tgttttagca atagtcttta acaacaaaat
    16861 agattaacca acccaatgaa aaacaaatga atattgccca tcaataagat aaatcagcga
    16921 ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg tactctacgg
    16981 cggttttatc gtcatgtaga gaaattatga tgtccaccac gttatttaaa gatttcacct
    17041 tcgaagccgc tcaccgcttg ccgcacgtcc cacaagggca taaatgtggt cgcctgcacg
    17101 ggcattcctt tatggtgcga ctggaaatta ccggggaagt cgatccgcat acgggctgga
    17161 ttatcgattt cgctgaactg aaagcggcgt ttaaaccaac ttatgagcgc ctcgatcacc
    17221 attatctcaa tgatattcct ggtctggaaa acccaaccag cgaagtttta gcaaaatgga
    17281 tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta aaagaaacct
    17341 gcaccgcagg ttgtatctat cgcggcgaat gataagagtg tgtcggcggt caatttccct
    17401 taagtaacgc tatgttaggg tgttgtgttc tggatatctg gggcatgaca tggaagacga
    17461 ctgcgacatt attattattg gtgccggtat tgctggcacc gcttgcgcgt tacgctgcgc
    17521 gcgagcgggt ttatccgttc tgttactgga acgagcggag attcccggca gcaaaaatct
    17581 ttccggcggg cgtttatata cccatgcgct cgcggaactc ctcccgcaat ttcacctgac
    17641 cgcgcctctt gaacgacgca tcactcacga aagtctttct ctgttaacgc cagatggcgc
    17701 aacgacgttt tccagcttac agcccggcgg tgaatcctgg agtgtattac gtgcacgatt
    17761 cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc ccggtgcgac
    17821 ggtagatgca ctgtatgaag aaaacggcag ggtgtgtggt gtcatttgtg gtgacgatat
    17881 tcttcacgcc cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg cggaacgcca
    17941 cgggttagtg actcgtcctg ctggcgaagc gatggcattg gggatcaaag aagtgctgac
    18001 gctggaacca tccgctattg aagaacgttt tcatctggag aataacgaag gcgcagcgtt
    18061 gttgttcagc ggcgggatct gtgatgactt acccggcggc gcatttcttt atactaatca
    18121 acaaacgctc tcgttaggga ttgtttgccc gctctcctcc cttacgcaaa gtcgtgttcc
    18181 ggcaagcgag ctgctggctc gctttaaaac gcatccggca gtgcgcccgc ttatcaaaaa
    18241 cacggaatca ctggagtatg gtgcgcatct ggtgccagaa ggtggcctgc gcagtatgcc
    18301 ggtgcaatac gccggtaacg gctggctgct ggtgggcgat gcgttgcgca gttgcgtcaa
    18361 taccggaatt tccgtgcgcg gcatggatat ggcgctaact ggcgcgcagg cggcggcaca
    18421 aacgctgata agcgcctgcc aacaccgcga gccgcaaaat ctgtttccgc tttatcatca
    18481 caacgtagag cgcagcctgc tgtgggatgt tctacagcgt tatcagcatg ttccggcgct
    18541 tttgcaacgt cctggctggt atcgggtgtg gcctgggtta atgcaggata tttcccgtga
    18601 tttatgggat caggggaata cacctgttcc accgctgcgc cagttgctct ggcgtcattt
    18661 acgtcgtcac ggcttgtggc atctgacggg cgatgttatc aggagtctgc gatgtctgta
    18721 gcccgtaatc tctggcgcgc tgccgatgcg ccgcacattg ttccagctga ctccgttgag
    18781 cgccagacgg cacaacggtt gattaacgcc tgtccggctg gtcttttttc gctcacacct
    18841 gaaggtgact tacgcgttga ttatcacggc tgcctggagt gtggcacctg ccgtttgctg
    18901 tgcgacgaat caacactaca acagtggcgc tatccgcctt ccggattcgg catcacctac
    18961 cgctttggat aaataacaag gataatttat gtccctctta cacctgctcc gccagaatcc
    19021 ggtgattgct gccgttaaag ataatgccag cctgcaactg gcaatcgact ctgaatgcca
    19081 gtttatttcc gtgttgtacg gcaatatctg caccattagt aatatcgtta aaaagattaa
    19141 gaacgccggg aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt caaataaaga
    19201 agtggtgatt cagtttttga aactggtgac cgaggcggac ggaattatca gtaccaaagc
    19261 ctcaatgctg aaagcagcaa gagcggaagg ttttttctgt attcatcgac tgtttattgt
    19321 tgattcgatt tcgtttcaca acattgataa gcaagttacg caatcgaatc cggattgtat
    19381 tgagatcctg ccaggctgca tgcctaaagt gctgggctgg gtgacggaga aaatccgcca
    19441 accgctgatt gctggcgggc tggtgtgcga tgaagaagat gcgcgtaatg cgattaacgc
    19501 gggtgttgtg gcgctttcca ccacgaatac tggggtctgg acgttagcga aaaaattgct
    19561 ttgacgggat aatcgtaaca agttgaattt ggtgtgattt gtaggctgca cgccacatcc
    19621 gacattcagc gcctgatgcg acgctt
//
LOCUS       DADTFG010000091        19159 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.091, whole genome shotgun sequence.
ACCESSION   DADTFG010000091 DADTFG010000000
VERSION     DADTFG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.091"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            420..1559
                     /locus_tag="J1138_002990"
     CDS             420..1559
                     /locus_tag="J1138_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBA8454759.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1565..2008
                     /gene="wzb"
                     /locus_tag="J1138_002991"
     CDS             1565..2008
                     /gene="wzb"
                     /locus_tag="J1138_002991"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBA8454760.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFTAVYTLLERSARQWAQALNAEQV"
     gene            2011..4173
                     /gene="wzc"
                     /locus_tag="J1138_002992"
     CDS             2011..4173
                     /gene="wzc"
                     /locus_tag="J1138_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBA8454761.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRNRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4263..5102
                     /gene="wcaA"
                     /locus_tag="J1138_002993"
     CDS             4263..5102
                     /gene="wcaA"
                     /locus_tag="J1138_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBA8454762.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWIPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5105..5593
                     /gene="wcaB"
                     /locus_tag="J1138_002994"
     CDS             5105..5593
                     /gene="wcaB"
                     /locus_tag="J1138_002994"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBA8454763.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5590..6807
                     /gene="wcaC"
                     /locus_tag="J1138_002995"
     CDS             5590..6807
                     /gene="wcaC"
                     /locus_tag="J1138_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBA8454764.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALSDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSVLNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6782..8002
                     /gene="wcaD"
                     /locus_tag="J1138_002996"
     CDS             6782..8002
                     /gene="wcaD"
                     /locus_tag="J1138_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBA8454765.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWCAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            8083..8829
                     /gene="wcaE"
                     /locus_tag="J1138_002997"
     CDS             8083..8829
                     /gene="wcaE"
                     /locus_tag="J1138_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBA8454766.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8845..9393
                     /gene="wcaF"
                     /locus_tag="J1138_002998"
     CDS             8845..9393
                     /gene="wcaF"
                     /locus_tag="J1138_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBA8454767.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGHPAVVIRKRVETE"
     gene            9420..10541
                     /gene="gmd"
                     /locus_tag="J1138_002999"
     CDS             9420..10541
                     /gene="gmd"
                     /locus_tag="J1138_002999"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBA8454768.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKDYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10544..11509
                     /gene="fcl"
                     /locus_tag="J1138_003000"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10544..11509
                     /gene="fcl"
                     /locus_tag="J1138_003000"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBA8454769.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11512..11991
                     /locus_tag="J1138_003001"
     CDS             11512..11991
                     /locus_tag="J1138_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBA8454770.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDEMLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11988..13202
                     /gene="wcaI"
                     /locus_tag="J1138_003002"
     CDS             11988..13202
                     /gene="wcaI"
                     /locus_tag="J1138_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBA8454771.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKVAQLA
                     TAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADVDAL
                     RNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLEKMA
                     QQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVI
                     TAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKE
                     NVLRQFINDIRG"
     gene            13205..14641
                     /gene="cpsB"
                     /locus_tag="J1138_003003"
     CDS             13205..14641
                     /gene="cpsB"
                     /locus_tag="J1138_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBA8454772.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNSVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSLQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14834..16204
                     /gene="cpsG"
                     /locus_tag="J1138_003004"
     CDS             14834..16204
                     /gene="cpsG"
                     /locus_tag="J1138_003004"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HBA8454773.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINFKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16259..17653
                     /gene="wcaJ"
                     /locus_tag="J1138_003005"
     CDS             16259..17653
                     /gene="wcaJ"
                     /locus_tag="J1138_003005"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBA8454774.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEASGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17655..19133
                     /gene="wzxC"
                     /locus_tag="J1138_003006"
     CDS             17655..19133
                     /gene="wzxC"
                     /locus_tag="J1138_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HBA8454775.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFIFTV
                     VSAHFWPLAMTAILGYLVNSVVRTLLFGYFGRKIYRPGLHFSLVSVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKAWVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSSALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT         4596 a         4775 c         5317 g         4471 t
ORIGIN      
        1 cttaatataa ttcttaaaaa tagccaattg ccgaattgtt accttgcctg ctattccgtt
       61 agctgtaaca cttcctcctg cattattgga gagccaatat tcaattacca taatgtcacc
      121 ttatattcgg aagttaattt ctgcaataac cctttctcat caacgactgt atgcagagag
      181 ggtttgaaaa attaaactaa atcagtgtat tggtagctaa aaagccaggg gcggtagcgt
      241 gtctggatgc ctgaaagacc agtctgaatt attctgtcaa tagcctgcgg atgaaatcaa
      301 tttttttagg actgatgcca gttaaatttt tattcaatta actgcaaggt aaatgtgcat
      361 ggcgacattg ctattaatag tgcacaggat aattactctg ccaaagtgat aaataaacaa
      421 tgatgaaatc caaaatgaaa ttgatgccat tattggtgtc agtaaccttg ataagcggtt
      481 gcacagtact tccgggcagc aatatgtcga cgatggggaa agacgtcatc aaacagcagg
      541 acgctgattt cgacctcgac aaaatggtga atgtttatcc gttaaccccg cgcctgattg
      601 atcaattacg cccacgaccc aacgtagcgc gccccaatat gacgctggaa agtgagatcg
      661 cgaattacca gtatcgcgtc gggccggggg acgttcttaa tgtcaccgtc tgggatcacc
      721 cggaactcac cacgccagcc ggtcagtacc gcagctccag cgacaccggc aactgggtac
      781 agcctgacgg caccatgttc tacccgtata tcggcaaggt tcacgtcgtg gggaaaacgc
      841 tcgctgaaat ccgcagtgat attaccgggc gcttagcgac gtacatcgct gacccgcagg
      901 tggacgttaa tatcgccgcc ttccgctcgc aaaaagccta tatctccggt caggtgaata
      961 aatccggtca acaggccatt accaacgtgc ctctgaccat tctcgacgct atcaacgccg
     1021 caggtggcct gaccgacacc gctgactggc gcaacgtggt gctaacacac aatggtcgtg
     1081 aagagcgcat ttctttgcag gcgctgatgc aaaacggcga cctcaatcag aaccgactgc
     1141 tttaccccgg cgatattctt tacgtgccac gtaatgatga tctgaaagtg tttgtgatgg
     1201 gtgaagtgaa gaaacagagc accctgaaaa tggactttag cggcatgacc ctgactgaag
     1261 ccctgggcaa tgctgaaggc atcgacatga ccacctccaa cgccagcggc atctttgtca
     1321 ttcgtccgct gaaaggcgag agcgggcgta acggcaagat tgccaatatc taccagctgg
     1381 atatgtccga tgcgacgtcg ctggtaatgg cgacagaatt ccgcctgcaa ccttatgacg
     1441 tggtgtatgt caccaccgcc ccggtatccc gctggaaccg tctgatcaat cagttgctgc
     1501 caactattag cggtgtccgt tacatgacgg atacagccag cgacattcat aactggtaat
     1561 cgtcatgttt aacaacatct tagttgtctg tgtcggcaat atttgccgtt ccccgacggc
     1621 ggaacgctta ctgcaacgtt atcacccgga gctaaaagtg gagtccgctg gactcggcgc
     1681 gctggtcggt aagggcgctg atcctaccgc tatcagcgtc gccgcagaac atcaactgtc
     1741 cctggaaggt cattgcgcac ggcaaatcag ccgccgtctg tgtcgcaact acgacctgat
     1801 tctgaccatg gaaaagcgcc atatcgaacg cttatgcgag atggcaccag agatgcgcgg
     1861 caaagtgatg ctgtttggtc actgggataa cgaatgtgaa atccccgatc cgtatcgcaa
     1921 aagccgggaa acgtttacag cggtgtacac attacttgaa cggtctgccc gccagtgggc
     1981 gcaggcattg aacgcagagc aggtataaga atgacagaaa aagtaaaaca acatgccgct
     2041 ccggtaacgg gcagtgatga aatcgatatt ggtcgcctgg tcggcaccgt cattgaagca
     2101 cgctggtggg tgattggcat taccgccgta ttcgccctct gtgccgtggt ttacaccttc
     2161 ttcgctacgc cgatttatag cgccgacgca ctggtacaaa tcgagcaaag cagcggcaat
     2221 tcgctggtgc aggatatcgg atcggcatta gccaacaaac cgcctgcatc ggacgccgag
     2281 atccagttga ttcgttcgcg cctggtgctt ggtaaaacgg tggacgatct cgaccttgat
     2341 attgcggtga gcaaaaacac gttcccgatt ttcggtgcgg gctgggagcg cctgatggga
     2401 cgccagaacg agacggtgaa agtgaccacc tttaaccgac cgaaggagat ggcggatcag
     2461 gtgtttacgc ttaatgtgct ggacaacaaa aactacaccc tgagcagcga tggcggcttt
     2521 agcgcccgtg ggcaagcggg ccagatgctg aaaaaagaag gcgtcacgct gatggttgaa
     2581 gccattcacg ccagcccggg cagtgagttt accgtcacca aatactccac gctggggatg
     2641 atcaaccaac tgcaaaacag cctgacggta acggagaacg gcaaagacgc aggcgttttg
     2701 agcctgactt ataccggtga agatcgcgaa cagatccgcg acattcttaa cagcatcgcc
     2761 cgtaactatc aggaacaaaa tattgagcgc aaatcggcgg aagcgtcgaa aagcctcgct
     2821 ttccttgcgc aacagttacc ggaagtacgt aaccgccttg atgttgccga aaacaaactg
     2881 aatgccttcc gtcaggataa agattcagtt gatctgccgc tggaagcgaa agcggtgctc
     2941 gattcgatgg tgaacatcga cgcgcagttg aacgaactta cctttaaaga agcggaaatc
     3001 tccaagctgt acaccaaagt tcacccggcg taccgcacgc tgctggagaa acggcaggcg
     3061 ctggaagacg aaaaagccaa acttaatggt cgcgtaacgg cgatgccgaa aacccagcag
     3121 gaaattgtgc gtctgacccg cgatgtcgag tctggtcagc aggtctatat gcagctgctg
     3181 aataaagagc aggagctgaa aatcaccgaa gccagcaccg tcggcgatgt gcgcattgtt
     3241 gacccggcaa tcacccagcc aggtgtactg aaaccgaaga aggggctgat tatcctcggg
     3301 gcgattatcc ttggcctgat gctctctatc gtgggtgtgc tgctgcgctc gttgtttaat
     3361 cgcggtatcg aaagcccgca ggtgctggaa gaacacggta tcagcgtcta tgccagcatc
     3421 ccgctgtcgg agtggcagaa agcgcgcgat agcgtcaaaa ccatcaaagg gattaaacgc
     3481 tataaacaga gccagctact ggcggtgggg aatccaaccg atctggcgat tgaagccatc
     3541 cgcagtctgc gtaccagtct gcacttcgcg atgatgcagg cacagaacaa tgtgttgatg
     3601 atgaccgggg ttagcccgtc aatcggtaaa acctttgtct gcgccaacct ggcggcggtg
     3661 attagccaga ccaataaacg ggtgttgttg atcgactgcg atatgcgcaa aggctacacc
     3721 cacgagctgt tgggcactaa taacgttaat ggcctgtcgg aaattctgat tggacagggc
     3781 gatattacta ccgctgctaa accgacctct attgccaaat ttgacctgat cccgcgcggt
     3841 caggtgccgc caaatccttc tgaactgttg atgagcgaac gctttgccga actggtgaag
     3901 tgggcgagta aaaactacga cctggtgttg attgatacgc cgccgattct ggcagtgacc
     3961 gatgcggcta tagttggtcg tcatgtcgga accacgttga tggtggcgcg ttatgcggtc
     4021 aacacattga aagaagtgga aaccagtctg agccgctttg agcaaaacgg tattccggtg
     4081 aaaggggtga ttctgaactc catcttccgc cgcgccagcg cgtatcagga ttatggctat
     4141 tacgaatacg aatataagtc ggatgcgaaa taacgacgct tgcattcgca ccgccccgta
     4201 ggccggataa ggcgttcacg ccgcatccgg caagcaaacc agctcataag ccagaacaac
     4261 ctatgaaaga caatccgctg atttcaatct atatgccgac ctggaaccgc caacaactgg
     4321 ctattcgggc gataaaatcg gtcctgcgcc aggactacaa caactgggag atgatcatcg
     4381 tggatgattg ctccacctcc tgggaacaac tacaacagta cgtcaccgcc ctcaacgatc
     4441 cgcgtatcac ttacattcac aacgacatta acagcggggc gtgcgcggta cgtaaccagg
     4501 cgattatgct ggcgcaaggg gaatacatca ccgggatcga tgacgacgat gaatggatac
     4561 ccaaccgcct gagcgtcttc ctcgcccata aacagcaact ggtcacacac gcctttttgt
     4621 acgctaacga ctacgtttgc cagggcgaag tttattccca accggcaagc ttgccactgt
     4681 atcccaaatc gccgtattca cgccgcctgt tctataaacg gaatatcatt ggcaatcagg
     4741 tctttacctg ggcgtggcgt ttcaaagagt gtttgtttga taccgaactg aaagcggcac
     4801 aggattacga catcttcctg cggatggtgg tggagtatgg cgaaccgtgg aaagtggaag
     4861 aggcgacgca gatcctgcat atcaatcacg gcgaaatgca gatcacctcg tcaccgaaaa
     4921 aattctccgg atacttccac ttttaccgta agcacaaaga caaatttgac cgcgccagca
     4981 aaaaatatca gctgtttacc ctctaccaga tccgcaataa acgcatgacc tggcgcacct
     5041 tgttgacgct gctgtcggtg cgtaacggca aacgtctggc tgatggtatt cgggggcgct
     5101 aaagatgctg gaagatctgc gcgccaacag ctggagttta cgcccgtgct gcatggttct
     5161 cgcctatcgc gtcgctcatt tttgttcggt gtggcgcaaa aagaacgtcc tcaacaatct
     5221 gtgggcggcc ccgctgctgg tgctgtatcg cattatcacc gaatgctttt tcggttatga
     5281 aatccaggcc gccgcgacta taggccgacg ctttactatc catcacggtt acgccgtggt
     5341 gatcaataaa aacgtggtcg cgggggatga tttcaccatt cgtcacggcg tcactatcgg
     5401 caatcgtggt gccgataaca tggcatgtcc acacattggc aacggcgtcg aactcggtgc
     5461 caacgtcatt attcttggtg atatcacgct tggtaacaac gtcaccgtgg gcgcgggcag
     5521 cgtggtgctc gactctgtcc cggacaacgc gctggtggtg ggagaaaaag cgcgagtgaa
     5581 ggtaattaaa tgaatattct gcaatttaat gtgcgactgg cggaaggcgg ggccgcaggt
     5641 gtggcgttag atctccacca gcgtgcgctg caacaggggc tggcgtcaca ttttgtctac
     5701 ggctacggca aaggcggcaa agagagcgtc agccatcaaa actatccgca ggtcatcaaa
     5761 catacgccgc ggatgaccgc gatggcgaac attgcgttgt ttcgtctgct taatcgcgat
     5821 ctgttcggca atttcaatga gttatatcgc accattactc gcacaccggg cccggtggtt
     5881 ctgcattttc atgtgctgca cagctactgg ttgaatctta agagcgtggt gcgcttttgt
     5941 gaaaaagtga aaaaccataa accggatgtc actctggtct ggacactgca cgaccactgg
     6001 agcgttaccg gacgctgcgc ctttaccgac ggttgcgaag gctggaaaac gggctgccag
     6061 aaatgcccga ccttaaataa ttatccgccg gtgaagattg atcgcgcaca ccaactggtg
     6121 gcgggcaaac ggcagttatt tcgcgagatg ctggcgctgg gctgtcagtt tatttctccc
     6181 agccagcatg tggctgatgc tttcaatagt ctgtacggtc cagggcgttg ccggattatc
     6241 aataacggta ttgatatggc aaccgaagcg attctggcgg atctgcctcc ggtgcgcgaa
     6301 acccagggca agccgaaaat cgcagtggtg gcgcatgatc tgcgttacga cggcaaaact
     6361 aaccagcaac tggtgcgcga gatgatggcg ctgagcgaca aaatcgaact gcatactttc
     6421 ggtaagttct cgccgttcac cgctggtaac gttgttaatc acggctttga aactgacaag
     6481 cgcaagttaa tgagcgtgct caatcagatg gatgcgctgg tgttcagttc tcgcgtcgat
     6541 aactacccgc tgattttgtg tgaggcgtta tcgattggcg tgccggtgat cgccacccat
     6601 agcgatgcgg cgcgggaagt gctgcaaaaa tccggtggta aaaccgtcag cgaagaagat
     6661 gtgctgcaac tggtgcagtt aagcaaaccg gaaatcgcac aggcgatatt tggtaccacg
     6721 ctggctgaat tcagccagcg cagccgcgcc gcctacagtg gacaacagat gctggaggag
     6781 tatgtcaact tctatcagaa tctgtagcta cctgctgctg ccgctgatct acctgctggt
     6841 taacgtcaaa atcgcccagc ttggcgaaag tttccccatc accatcgtca cttttttacc
     6901 tgtcttgtta ctgctgtttt tagagcgcat cagcgttaaa aaattgatga ttgccttagg
     6961 gattggcgcg gggctcacgg cgtttaacta cctgtttggt cagtcgttgg atgccagtaa
     7021 atatgtcact tcaaccatgc tgtttgtcta tattgtgatc attattggca tggtgtggag
     7081 tattcgcttt aaaacaattt cgccacacaa ccatcgcaag atattacgtt tcttttatct
     7141 ggtggtcggg ttggtggtgg tgctggcggc ggtggagatg gcgcaaatta tcctcaccgg
     7201 cggtagcagt attatggagt cgatttcgaa atatctgatt tacagcaaca gctatgtgct
     7261 gaatttcatt aaattcggcg gcaagcgcac gacagcactt tatttcgaac cggcattttt
     7321 cgctctggca ttaatctcaa tttggctcag catcaaacag tttggtatca aaacgcctaa
     7381 aaccgatgct atgattctcg cagggataat attatccgga tcgttttcag gggttatgac
     7441 ctttatcctt ttttatttgc tggagtgggc atttcaatat ctgaataaag aggcgattaa
     7501 gaaaaagtta ccgttagcat tgatttctct ggctgtattc ctggttggtg tggtaatcgc
     7561 gttcccttat atttccaccc gtctgggcga tttaggtaca gaaggatcgt catcgtatta
     7621 tcgtattgtc ggtccgctgg tgatggtcgg ttattctttg acccatattg acggtgtagt
     7681 cagatttggc tcactttatg aatatgtcgc atcattcgga atatttaacg gtgcggatgt
     7741 cggaaaaacc atagacaatg gtttgtatct gctgattatt tatttttcct ggtgcgcagt
     7801 gtttttatcg ctgtggtaca tgggaaaagt gataaaaatg atgatcaacg cttttggtga
     7861 taaccgcaat tttcgcgtgc aattatatct ttttactccg gtatcgctgt tttttaccgg
     7921 ttcgatattt agcccggaat atgcgttttt aatcgtctgc ccgtttattt tgcgaaaagc
     7981 gttaaatata caccctaaat aattcgagtt gcaggaagtg ggtgagcgag gaaaaccaac
     8041 gcacatgcag cttgaagtat gacgggtatt atgaggtaaa acatgttgct tagcataatc
     8101 actgtcgcgt ttcgtaacct cgaaggaata gtcaaaacac acgcctcgct ggcgcatctg
     8161 gcgcaggcgg acgatatcag cttcgaatgg attgttgtcg atggcggttc caatgacggc
     8221 acccgtgagt atctggaaaa tctcaatggt atctataacc tacgctttgt cagcgagcca
     8281 gataacggta tctacgacgc catgaacaaa ggtattgaaa tggcgcaagg caagttcgcg
     8341 ctgtttctca attcaggcga tatttttcat caggatgccg caaattttgt ccgtaagtta
     8401 aaagtgcaaa aagataacat gatgatcacc ggcgatgcgc tgctggattt tggcgacggg
     8461 cataaaatta aacgtagcgc caaaccgggt tggtatattt accacagcct gcccgccagt
     8521 catcaggcga tatttttccc ggtgtccggc ttgaagaaat ggcgttatga cctggaatat
     8581 aaagtttctt ccgattacgc gctggcggcc aaaatgtata aagccggtta tgcatttaaa
     8641 aaactcaatg gcctggtgtc tgaattttct atgggtgggg tatctaccac caataatatg
     8701 gaattgtgtg ctgacgccaa aaaagtccaa cggcagatat tgcacgtgcc tggcttttgg
     8761 gctgaattat cctggcattt acgccaacgt acgacctcaa agacgaaagc cttatataac
     8821 aaaagctgaa tataagggaa aacgatgcag gatttaagtg gattctcggt gccgaaaggg
     8881 ttccggggtg gcaacgctat taaagtgcaa ttgtggtggg cagtacaggc aacaatattt
     8941 gcctggtcgc cacaagtatt gtatcgctgg cgggcatttt tattacgttt attcggcgca
     9001 aaaataggaa aaaacgtagt cattcgtccg tcagtaaaaa ttacctatcc gtggaaatta
     9061 acgttaggtg attacgcgtg ggtcggcgat gaggtcaatt tatataccct cggtgaaata
     9121 accattggcg cacattcggt gatatcgcaa aaaagttatt tatgcactgg tagccacgac
     9181 catgcaagtc aacatttcac cattaacgcc acgcctattg tgattggcga gaaatgctgg
     9241 ctggcaaccg atgtctttgt tgcccctggc gtcacgatcg gcgacggcac cgtcgtgggt
     9301 gcgcgaagca gtgtttttaa atcgcttccg gcaaatgtgg tttgtcgggg gcaccccgca
     9361 gtggtgatac gcaaacgcgt tgaaactgaa taaattcaaa aaatacagag gaataagaca
     9421 tgtcaaaagt cgctctcatc accggtgtaa ccggacaaga cggttcttac ctggcagagt
     9481 ttctgctgga aaaagattac gaggtgcatg gtattaagcg tcgtgcatcg tcattcaaca
     9541 ccgagcgcgt ggatcacatt tatcaggatc cgcacacctg caacccgaaa ttccatctgc
     9601 attatggcga cctgagtgat acctccaacc tgacgcgcat tttgcgtgaa gtgcagccgg
     9661 atgaagtgta caacctgggc gcaatgagcc acgttgcggt ttcttttgag tcaccggaat
     9721 ataccgcaga cgtcgatgcg atgggtacgc tgcgcctgct ggaggcgatc cgcttcctcg
     9781 gtctggaaaa gaaaacccgt ttctatcagg cttccacttc tgaactgtac ggtctggtgc
     9841 aggaaattcc gcagaaagag accacgccgt tctacccgcg atctccgtat gcggttgcca
     9901 aactgtacgc ctactggatc accgttaact accgcgaatc ctacggcatg tacgcctgta
     9961 acggcattct ctttaaccat gaatccccgc gtcgtggcga gactttcgtt acccgcaaaa
    10021 tcacccgcgc aatcgccaac atcgcccagg ggctggagtc gtgcctgtat ctcggcaata
    10081 tggattccct gcgtgactgg ggccacgcca aagactacgt aaaaatgcag tggatgatgc
    10141 tgcaacagga acaaccggaa gatttcgtta ttgccaccgg tgttcagtac tccgtgcgtc
    10201 agttcgtgga aatggcggcg gcacaactgg gcatcaaact gcgctttgaa ggcacgggtg
    10261 ttgaagagaa gggcattgtg gtttccgtca ccgggcatga cgcgccgggc gttaaaccgg
    10321 gtgatgtgat tatcgccgtt gacccgcgct acttccgtcc ggctgaagtt gaaacgctgc
    10381 tcggtgaccc gaccaaagcg cacgaaaaac tgggctggaa accggaaatc accctcagag
    10441 agatggtgtc tgaaatggtg gctaatgacc ttgaagcggc gaaaaaacac tctctgctga
    10501 aatctcacgg ctacgacgtg gcgatcgcgc tggagtcata agcatgagta aacaacgaat
    10561 ttttatcgct ggccatcgcg ggatggtcgg ttctgccatc aggcgacagc tcgaacagcg
    10621 cggtgatgtg gagctggtat tacgcacccg cgacgagctg aacctgctgg acagccgcgc
    10681 cgtgcatgat ttctttgcca gcgaacgtat tgaccaggtc tatctggcgg cggcgaaagt
    10741 gggcggcatt gttgccaaca acacctatcc ggcggatttc atctatcaga acatgatgat
    10801 tgagagcaac atcattcacg ccgcgcatca gaacgacgtg aacaaactgc tgtttctcgg
    10861 atcgtcctgc atctacccga aactggcaaa acagccgatg gcagaaagcg agctgttgca
    10921 gggcacgctg gagccgacca acgagcctta cgccattgcc aaaatcgccg ggatcaaact
    10981 gtgcgaatct tacaatcgcc agtacggacg agattaccgt tcagtcatgc cgaccaacct
    11041 gtacgggccg cacgacaact tccacccgag taactcgcat gtgatccctg cattgctgcg
    11101 ccgcttccac gaggcgacgg cacagaatgc accggacgtg gtggtgtggg gcagcggtac
    11161 accgatgcgc gaatttctgc atgtcgatga tatggcggcg gcgagcattc acgtgatgga
    11221 gctggcgcac gaagtctggc tggagaacac ccagccgatg ctgtcgcaca ttaacgtcgg
    11281 cacgggcgtt gactgcacta tccgcgagct ggcgcaaacc atcgccaaag tggtgggtta
    11341 caaaggtcgg gtggtttttg atgccagtaa accggatggc acgccgcgca aactgctgga
    11401 tgtgacgcgc ctgcatcagc ttggctggta tcacgaaatc tcactggaag cggggcttgc
    11461 cagcacttac cagtggttcc ttgagaatca agaccgcttt cgggggtaat gatgttttta
    11521 cgtcaggaag actttgccac ggtagtgcgc tccactccgc ttgtctctct cgactttatt
    11581 gtcgagaaca gtcgcggcga gtttctgctt ggcaaaagaa ccaaccgccc ggcgcagggt
    11641 tactggtttg tgccgggagg gcgcgtgcag aaagacgaaa tgctggaagc cgcatttgag
    11701 cggctgacga tggcggaact ggggctgcgt ctgccgataa cagcaggcca gttttacggt
    11761 gtctggcagc acttttatga cgacaacttc tccggcacgg atttcaccac tcactatgtg
    11821 gtgctcggtt ttcgcttcag agtagcggaa gaagagctgt tactgccgga tgagcagcat
    11881 gacgattacc gctggctgac gccggacgca ctgctcgcca gtgacaatgt tcacgctaac
    11941 agccgcgcct attttctcgc tgagaagcgt gccggagtac ccggattatg aaaatactgg
    12001 tctacggcat taactactcg ccggagttaa ccggcatcgg caaatacacc ggcgagatgg
    12061 tggaatggct ggcggcgcaa ggtcatgagg tgcgggttat taccgcaccg ccttactacc
    12121 cgcagtggca ggtgggcgag aactattccg cctggcggta caaacgggaa gagggggccg
    12181 ccacggtgtg gcgctgcccg ctgtacgtgc caaaacagcc gagcaccctg aaacgcctgt
    12241 tgcatctcgg cagttttgcc gtcagcagtt ttttcccgtt gatggcgcaa cgtcgctgga
    12301 agccggatcg cattatcggc gtggtgccaa cgctgttttg cacgccggga atgcgcctgc
    12361 tggcgaagct ctctggtgcg cgtaccgtgc tgcatattca ggattacgaa gtggacgcca
    12421 tgctggggct gggccttgcc ggaaaaggca aagtggcaca gctggcaacg gcgttcgaac
    12481 gtagcggact gcataacgtc gataacgtct ccacgatttc gcgctcgatg atgaataaag
    12541 ccatcgaaaa aggcgtggcg gcggaaaacg tcatcttctt ccccaattgg tcggaaatcg
    12601 cccgttttca acatgttgca gacgccgatg ttgacgccct tcgtaaccag cttggcctgc
    12661 cggataacaa aaaaatcatt ctttactccg gcaatattgg tgaaaagcag ggactggaaa
    12721 acgttattga agctgccgat cgcctgcgcg atgaaccgct gatttttgcc attgtcgggc
    12781 agggcggcgg caaagcgcgg ctggaaaaaa tggcgcagca gcgtggactg cgcaacatgc
    12841 aattttttcc gctgcaatcg tatgacgctt tacccgcact gctgaagatg ggggattgcc
    12901 atctggtggt gcaaaaacgc ggcgcggcag atgccgtatt gccgtcgaaa ctgaccaata
    12961 ttctggcggt aggcggtaac gcggtgatta ccgcagaagc ccacaccgaa ctggggcagc
    13021 tttgcgaaac ctttccgggc attgcggttt gcgtggaacc ggaatcggtc gaggcgctgg
    13081 tggcggggat tcgtcaggcg ctcctgctgc ccaaacacaa cacggtggca cgtgaatatg
    13141 ccgaacgcac gctcgataaa gagaacgtgt tacgtcaatt tataaatgat attcggggat
    13201 aattatggcg cagtcgaaac tctatccagt tgtgatggca ggtggctccg gtagccgctt
    13261 atggccgctt tcccgcgtac tttaccccaa gcagttttta tgcctgaaag gcgatctcac
    13321 catgctgcaa accaccatct gccgcctgaa cggcgtggag tgcgaaagcc cggtggtgat
    13381 ttgcaatgag cagcaccgct ttattgtcgc ggaacagctg cgtcaactga acaaactcac
    13441 cgagaacatt attctcgaac cggcagggcg taacactgca ccggccattg cgctggcggc
    13501 gctggcggca aaacgtcata gcccggagaa cgacccatta atgctggtgc tggcggcgga
    13561 tcatgtgatt gccgacgaag atgcgttccg cgccgccgtg cgtaacgcca tgccgtatgc
    13621 tgaagcgggc aagctggtga ccttcggcat tgtgccggat ctacctgaaa ccggatatgg
    13681 ctatattcgt cgcggtgaag tgtctgcggg tgagcaggat acggtggcct ttgaagtggc
    13741 gcagtttgtc gaaaaaccga atctggaaac cgctcaggcc tatgtggcaa gcggcgaata
    13801 ttactggaac agcggcatgt tcctgttccg cgccggacgc tatctcgaag aactgaaaaa
    13861 atatcgtccg gatatcctcg atgcctgcga aaaagcgatg agcgccgtcg atccggatct
    13921 cgattttatt cgcgtggatg aagaagcgtt tctcgcctgc ccggaagagt cggtggatta
    13981 cgcggtcatg gaacgtacgg cagatgccgt tgtggtgccg atggatgcgg gctggagcga
    14041 tgtcggttcc tggtcctctt tatgggagat cagtgcccac accgccgagg gcaacgtttg
    14101 ccatggcgat gtgattaatc acaaaactga aaacagctat gtgtatgccg aatctggcct
    14161 ggtcaccacc gtcggggtga aagatttggt ggtagtgcag accaaagatg cagtgctgat
    14221 tgccgaccgt aactcggtgc aggatgtgaa aaaagtggtc gagcagatca aagctgatgg
    14281 tcgccatgag catcgggtgc atcgcgaagt gtatcgtccg tggggcaaat atgactctat
    14341 cgacgcgggc gaccgctacc aggtgaaacg catcaccgtg aaaccgggcg aaggcttgtc
    14401 gctacagatg caccatcacc gcgcggaaca ctgggtggtt gtcgcgggaa cggcaaaagt
    14461 cactatcgac ggtgatatca aactgcttgg tgaaaacgag tccatttata ttccgctggg
    14521 ggcgacgcat tgcctggaaa acccggggaa aattccgctc gatttaattg aagtgcgctc
    14581 cggctcttat ctcgaagagg atgatgtggt gcgcttcgcg gatcgctacg gacgggtgta
    14641 aacgtcgcat caggcgctgt ttgtcggatg cggtgtgaac gccttatccg acctacggtt
    14701 cgggtttgta ggcctgataa gacgcggcag cgtcgcatca ggcaatgaat gcgaaaccgc
    14761 gatgtaaata acgacaaaaa caaaattggc cgtttcggtc agggccaact attgcctgaa
    14821 aaagggtaac gatatgaaaa aattaacctg ctttaaagcc tatgatattc gcgggaaatt
    14881 aggcgaagaa ctgaatgaag atatcgcctg gcgcattggt cgcgcctatg gcgaatttct
    14941 caaaccgaaa accattgtgt taggcggtga tgtccgcctc accagcgaaa ccttaaaact
    15001 ggcgctggcg aaaggtttac aggatgcggg cgtcgacgtg ctggatatcg gcatgtccgg
    15061 caccgaagaa atctatttcg ccacgttcca tctcggcgtg gatggcggca ttgaagttac
    15121 cgccagccat aacccgatgg attacaacgg catgaagctg gtgcgcgaag gggctcgccc
    15181 gatcagcggc gataccggac tgcgcgatgt ccagcgtctg gcagaagcca acgactttcc
    15241 tcccgtcgat gaaaccaaac gcggtcgcta tcagcaaatc aatctgcgtg acgcttacgt
    15301 tgatcacctg ttcggttata tcaatttcaa aaaccttacg ccgctcaagc tggtgatcaa
    15361 ctccgggaac ggcgcagcgg gtccggtggt ggacgccatc gaagcccgct ttaaagccct
    15421 cggcgcaccg gtggagttaa tcaaagtgca taacacgccg gacggcaatt tccccaacgg
    15481 tattcctaac ccgttgctgc cggaatgtcg cgacgacacc cgcaatgcgg tcatcaaaca
    15541 cggcgcggat atgggcattg cctttgatgg cgattttgac cgctgtttcc tgtttgacga
    15601 aaaagggcag tttattgagg gctactacat tgtcggcctg ctggcagaag cgttcctcga
    15661 aaaaaatccc ggcgcgaaga tcatccacga tccacgtctc tcctggaaca ccattgatgt
    15721 ggtgacggcc gcgggcggca cgccggtgat gtcgaaaacc ggacacgcct ttattaaaga
    15781 acgtatgcgc aaggaagacg ccatctacgg tggcgaaatg agcgcccacc attacttccg
    15841 tgatttcgct tactgcgaca gcggcatgat cccgtggctg ctggtcgccg aactggtgtg
    15901 cctgaaagga aaaacgctgg gcgaactggt gcgcgaccgg atggcggcat tcccggcaag
    15961 cggtgagatc aacagcaaac tggcgcaacc cgttgaggcg attaaccgcg tggaacagca
    16021 ttttagccgt gaggcgctgg cggtggatcg caccgatggc atcagcatga cctttgccga
    16081 ctggcgcttt aatctgcgct cctccaacac cgaaccggtg gtgcggttga atgtggaatc
    16141 acgcggtgat gtgccgctga tggaagcgcg aacgcgaact ctgctgacgt tgctgaacga
    16201 gtaatgtcgg atcttccctt accctactgc gggtaagggg ctaaaaacag gaacaacgat
    16261 gacaaatcta aaaaagcgcg agcgagcgaa aaccaatgca tcgttaatct ctatggtgca
    16321 acgcttttca gatatcacca tcatgtttgc cggactatgg ctggtttgcg aagcgagcgg
    16381 actgtcattc ctctacatgc acctgttggt ggcactgatt acgctggtgg tgttccagat
    16441 gctgggcggc atcaccgatt tttatcgctc atggcgcggt gttcgggcag cgacagaatt
    16501 tgctctgttg ctacaaaact ggacgttaag cgtaattttc agcgccggac tggtggcgtt
    16561 caacaatgat ttcgacacgc aattgaaaat ctggctggcg tggtatgggc tgaccagcat
    16621 cggactggtg gtttgccgtt catgtattcg tattggggcg ggctggctgc gtaatcatgg
    16681 ctataacaag cgtatggtcg cggtggcggg ggatttagcc gccggacaaa tgctgatgga
    16741 gagcttccgt aatcagccgt ggttagggtt tgaagtggtg ggcgtttacc acgacccgaa
    16801 accgggcggc gtttctaacg actgggcggg taatctgcaa cagctggtcg aggatgcgaa
    16861 agcgggcaag attcataacg tctatatcgc gatgcagatg tgcgacggcg cgcgagtgaa
    16921 aaaactggtc catcaactgg cggacaccac ctgttcggtg ctgctgatcc ccgatgtctt
    16981 taccttcaac attctccatt cccgtctcga agagatgaac ggcgttccgg tggtgccgct
    17041 gtacgacacg ccgctttccg gggttaaccg cctgataaaa cgtgcagaag acattgtgct
    17101 ggcgacgctt attctgctgc tgatctcccc ggtgctgtgc tgcattgcgc tggcggtgaa
    17161 actgagctca ccggggccgg ttattttccg ccagactcgc tacggcatgg atggcaagcc
    17221 aatcaaagtg tggaagttcc gttctatgaa agtgatggag aacgacaaag tggtgactca
    17281 ggcgacacag aacgatccgc gcgtcaccaa agtggggaac tttctgcgcc gcacctcgct
    17341 ggacgaactg ccgcagttta tcaatgtgct gactggcggg atgtcgattg tcggtccgcg
    17401 tccgcatgcg gtggcgcata acgaacagta tcgccagctc attgaaggct acatgctgcg
    17461 ccataaagtg aaaccgggca ttactggctg ggcgcagatt aacggctggc gcggcgaaac
    17521 cgacacgctg gagaaaatgg aaaaacgcgt cgagttcgac cttgagtaca tccgcgaatg
    17581 gagcgtctgg ttcgatatca aaatcgtttt cctgacggta ttcaagggct tcgttaacaa
    17641 agcggcatat tgatatgagc ttacgtgaaa aaaccatcag cggcgcgaag tggtcggcga
    17701 ttgccacggt gatcatcatc ggcctcggtc tggtgcaaat gaccgtgctg gcgcggatta
    17761 tagataacca ccagttcggc ctgctgactg tgtcgctggt gattatcgcg ttggcagata
    17821 cactttctga cttcggtatc gctaactcga ttattcagcg aaaagaaatc agccaccttg
    17881 aactcaccac gctgtactgg ctgaacgtcg ggctggggat cgtggtgtgc gtggcggtgt
    17941 ttttgttgag tgatgtcatc ggtgacgtgt tgaataaccc ggacctggca ccgttgatta
    18001 aaacattgtc gctggcgttt gtggttatcc cccacgggca acagttccgc gcgttgatgc
    18061 aaaaggagct ggagttcaac aaaatcggca tgatcgaaac cagcgcggtg ctggcgggct
    18121 tcatttttac ggtggttagc gcccatttct ggccgctggc gatgaccgcg attctcggtt
    18181 atctggtcaa tagtgtggtg agaacgctgc tgtttggcta ctttggccgc aaaatttatc
    18241 gccccggtct gcatttctcg ctggtgtcgg tggcaccgaa cttacgcttt ggtgcctggc
    18301 tgacggcgga cagcatcatc aactatctca acaccaacct ttcaacgctg gtgctggcgc
    18361 gtattctcgg cgctggcgtg gctgggggat acaacctggc gtacaacgtg gccgttgtgc
    18421 caccgatgaa gctgaacccg atcatcaccc gcgtgttgtt tccggcgttc gccaaaattc
    18481 aggacgatac agaaaagctg cgcgtcaact tctacaagct gctgtcggta gtggggatta
    18541 tcaactttcc ggcgctgctc gggctgatgg tggtgtcgaa taactttgta ccgctggtat
    18601 ttggcgaaaa gtggagcagc attattccgg tgctgcaatt gctgtgtatt gtggggctac
    18661 tgcgctccgt gggtaacccg attggttcgc tgctgatggc gaaagcgtgg gtcgatatca
    18721 gctttaaatt caacgtattc aaaacctttc tgtttattcc ggcgattgtt attggtgggc
    18781 agatggcggg cgcaatcggc gtcacgcttg gcttcctgct ggtgcagatt atcaacacca
    18841 ttctgagcta tttcgtgatg attaaaccgg tgctcggttc cagttatcgc cagtacatcc
    18901 tgagtttgtg gctgccgttt tatctctcgc tgccgacgct ggtggttagt tctgcgctag
    18961 gcattgtgct gaaagggcaa ctggcgctgg ggatgctgct ggcggtgcaa atagccgcgg
    19021 gcgtgctggc gtttgtggtg atgattgtgc tgtcgcgcca tccgctggtg gtggaagtga
    19081 agcgtcagtt ttgtcgcagc gaaaaaatga aaatgctttt acgggcgggg tgaatggttg
    19141 ttttcggttc cggcaacgg
//
LOCUS       DADTFG010000092        19153 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.092, whole genome shotgun sequence.
ACCESSION   DADTFG010000092 DADTFG010000000
VERSION     DADTFG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.092"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..4902
                     /locus_tag="J1138_003007"
     CDS             <1..4902
                     /locus_tag="J1138_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA8454776.1"
                     /translation="DNTILDGGTGTRITGDDATANNSGNTTVDGQGSTGTEIAGNNAV
                     VNQDGELDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGIQIDGDKAVVNNDGDSAV
                     SNGGTGTQVNGDEATVNNNGSTTVDGKDSTGTEINGDKAIVNNDGDSTILDGGTGTRI
                     TGDDATANNSGNTTVDGQGSTGTEIAGNNAVVNQDGLLDVSGGGHGIDITGDSATVIN
                     KGNITVTDKDSVGVLINGDRATFANTGHIDVNNSATGMSITTSEGAISQAGSMNVGDF
                     STGMALSGNNNSVTLAAKDLNVTGQKATGVNISGDNNAVDITGNILVDKDQTATNAVD
                     YFYEPSIGVNVSGNSNTVSLDGKLTVVADSELTSHIYADFDGSQENISGLVVSGDDNT
                     VYLNGGIQLVGEENQLTDGSTVASNRKGYGKTPVITVDGKSSIYLNGDSTINGDLPLA
                     YSGMIRLKNSAMIEIGTDATINMQVDSYDHYARSEAQIIFVESGAELINKGDIDTRNI
                     GFAAISGENSTGSNSGNITLSQYNYGLLANAGVGYFTTKGGSAVNNGTITAKVMEQES
                     VINLGASLGLNEANTFYSDANSMMGLDAFDHGYVSNESGGSIEMYGRGNVGMLAIDES
                     TAENAGQITLDALWVDADDTTTLRSNIGNDARSYAVGMAVGTNTYSGPRKNATAVNKQ
                     GGVITVYNAGIGMAAYGASNTVINEGIINLEKNANYDSSLGANSLIGMAAYKSGTAIN
                     EQSGVININADNGQAFYSDGTGTILNYGTICVNTNCLTGNDYNETDSYTSLLYTGGDV
                     ITAQNETQSLAQKATINDKKEGNVVNSGSLSGADIAISSGELVNTSTGIINNAIIIND
                     GELSNEGSVAKVTLNAGTFGNIGTVNSRMFQTGGTFNNQQGGVVQNGANLSKTAIANN
                     AGTWYLGASSSSDSNNASMMEIYNTAVFNNSGDFILNNSRNAVHLYQSGTFYNTGHML
                     ISGANYSGNAINYWNANNNGRFINSGTVDVTAKALATSGVDASTNHAYFWNQNSGIVN
                     FDKDSGVAVKFTHSNYVAQNDGTMNISGNNAIAMEGNKNAQLINNGTINLGAQGTTDT
                     GMIGMQLDSSATADAVIENNGTINIYANNSFAFSMLGSVGHLVNNGTVTIADGVTGAG
                     LIKQGNSVNIEGVNGNNGNNSEVHYADYTLPDVPDSSVSVAASNPSSDGSQNKLNGYV
                     VGTSSDGSAGKLKVNNASLKGVSVNTGFTAGTSSTSVTFDNVVQGSNLTDAETITSTS
                     VVWNAQGTTDASGNVDVTMTKNAYTDVATDSSVNTVAQVLDAGYTNNELYSSLNVGTT
                     AELNSALKQVSGSQATTVFNEARVLSNRFSMLSDAAPEVANGLAFNVVAKGDPRAELG
                     NDTQYDMMALRKSLTLTEHQNLSLEYGIARLDGNGSDTAGDNGVTGGYSQFFGLKHQM
                     AFDNGMSWNNALRYDVHNLDSSRSIAYGDINKTADANVKQQYLEFRSEGAKTFELREG
                     LNVTPYAGVKLRHTLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKEGWSAN
                     ATLEGGPNLSYVKSQRTASVSGAGSQRFNIDDGQSGGGFNSLATMGVKYSSQESALQV
                     DAFHWKEDGISDKGVMLNFKKTF"
     gene            5122..5988
                     /gene="pdxI"
                     /locus_tag="J1138_003008"
     CDS             5122..5988
                     /gene="pdxI"
                     /locus_tag="J1138_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HBA8454777.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPTEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSISQKAG"
     gene            complement(6029..6634)
                     /gene="azoR"
                     /locus_tag="J1138_003009"
     CDS             complement(6029..6634)
                     /gene="azoR"
                     /locus_tag="J1138_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBA8454778.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            6835..10737
                     /gene="hrpA"
                     /locus_tag="J1138_003010"
     CDS             6835..10737
                     /gene="hrpA"
                     /locus_tag="J1138_003010"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBA8454779.1"
                     /translation="MTEQQKLTFTALQQRLDSMMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAIDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            11010..11810
                     /locus_tag="J1138_003011"
     CDS             11010..11810
                     /locus_tag="J1138_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBA8454780.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            12007..13446
                     /gene="aldA"
                     /locus_tag="J1138_003012"
     CDS             12007..13446
                     /gene="aldA"
                     /locus_tag="J1138_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBA8454781.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(13488..14489)
                     /gene="gap"
                     /locus_tag="J1138_003013"
     CDS             complement(13488..14489)
                     /gene="gap"
                     /locus_tag="J1138_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBA8454782.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            14678..15208
                     /gene="cybB"
                     /locus_tag="J1138_003014"
     CDS             14678..15208
                     /gene="cybB"
                     /locus_tag="J1138_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBA8454783.1"
                     /translation="MGNKYSKLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKCPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            15453..15626
                     /locus_tag="J1138_003015"
     CDS             15453..15626
                     /locus_tag="J1138_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454784.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(15698..15847)
                     /gene="hokB"
                     /locus_tag="J1138_003016"
     CDS             complement(15698..15847)
                     /gene="hokB"
                     /locus_tag="J1138_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBA8454785.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQSLYELRFRDGDKEVAALM
                     ACTSR"
     gene            16110..16553
                     /locus_tag="J1138_003017"
                     /pseudo
     CDS             16110..16553
                     /locus_tag="J1138_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(16591..17514)
                     /locus_tag="J1138_003018"
     CDS             complement(16591..17514)
                     /locus_tag="J1138_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8454786.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            17731..19074
                     /locus_tag="J1138_003019"
     CDS             17731..19074
                     /locus_tag="J1138_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA8454787.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQVIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETRACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         4968 a         4444 c         5113 g         4628 t
ORIGIN      
        1 gacaacacga tccttgatgg cggcaccgga acccgcatta cgggtgatga tgcgacggcc
       61 aacaacagtg gcaataccac cgttgacgga cagggctcga ccggtaccga aatcgcgggt
      121 aataacgcgg tggtgaacca ggacggcgag ctggatgtga gcggcggtgg tcacggtatt
      181 gatatcaccg gcgacagcgc aacggtggat aacaaaggcg gtatgaccgt caccgatccg
      241 gattcgattg gcattcagat cgacggcgac aaggcggttg tcaataacga cggtgacagt
      301 gccgtcagta acggcggcac cggcacgcag gttaacggcg acgaagccac cgtaaacaat
      361 aacggcagca ccaccgttga cggcaaggat tccacgggca ccgagataaa cggtgacaaa
      421 gccatcgtca acaacgacgg cgacagcacg atccttgatg gcggcaccgg aacccgcatt
      481 acgggtgatg atgcgacggc caacaacagc ggcaatacca ccgtagacgg tcagggttcg
      541 accggaactg aaatcgcggg taacaacgcg gtggtgaacc aggacggctt actggatgtg
      601 agcggcggcg gtcacggcat tgatatcact ggtgatagcg ccactgtaat caacaaaggc
      661 aatataactg tcacggataa agattcggtt ggtgtgctga ttaacggtga tcgagcgacc
      721 tttgccaata cgggtcatat tgacgtcaat aattcagcta ctggcatgag catcacgacc
      781 agcgaggggg ccatctccca ggcaggtagc atgaatgttg gtgacttttc aacaggcatg
      841 gcgctcagtg gtaataacaa cagtgtgacg ttggcggcga aagatctgaa cgtgaccggg
      901 cagaaggcaa cgggcgtcaa catctctggt gacaataatg ccgttgatat cactggtaat
      961 attttggtag ataaagatca aactgcgact aacgcggtag attattttta cgaaccgtct
     1021 attggtgtga acgttagcgg aaacagtaac actgtatcgc tagacggcaa gttaactgtg
     1081 gtggctgaca gtgaacttac ctcacatatt tatgctgact ttgatggaag ccaggagaat
     1141 atttccgggc ttgtcgtatc tggtgatgat aataccgtgt atctgaatgg tggtatccag
     1201 ctggttggtg aagaaaatca attgactgat ggttctaccg tcgcatcaaa ccgcaagggc
     1261 tatggcaaaa caccagtaat cactgttgat gggaagtcgt ctatttacct gaacggggac
     1321 tccacgatta acggtgattt acctctagcc tattcaggga tgattcggtt gaagaatagc
     1381 gccatgattg agatcggaac ggatgccacg attaacatgc aggtagatag ttatgatcac
     1441 tatgcgagat ctgaagctca aatcatcttt gtagagtctg gtgctgagtt aattaataaa
     1501 ggtgatattg atactcgaaa tattggtttt gccgctattt ctggtgaaaa ttcaacggga
     1561 agtaacagtg gcaatattac tctttctcag tacaactatg gcttgttagc aaatgcagga
     1621 gttggctatt tcacgactaa aggcggtagt gcggttaata atggtacgat tactgcgaaa
     1681 gtcatggaac aagaaagtgt cattaatttg ggagcgtctc tgggtctgaa tgaggcgaac
     1741 actttctact ctgatgcgaa cagtatgatg ggtcttgatg cctttgatca cggatacgtg
     1801 tccaatgaaa gcggtggcag cattgagatg tatggtcgcg gcaacgttgg tatgttggct
     1861 attgatgaga gtacagcaga aaacgctggg caaattacac tggatgccct gtgggttgat
     1921 gcagatgata ccaccacttt gcgcagcaat attggtaatg atgccaggag ctatgccgtt
     1981 ggtatggcag taggtacaaa tacttacagt gggccaagga aaaatgctac ggccgttaat
     2041 aaacagggtg gtgtaataac ggtttataat gctggaatag gtatggccgc ctatggtgcc
     2101 agcaataccg tgattaacga gggtattatc aacctggaga aaaatgctaa ctacgatagt
     2161 agtctgggag caaattctct gatcggtatg gccgcctata aaagtggtac tgccattaat
     2221 gaacagtccg gcgtaattaa tatcaatgct gacaatgggc aggcgtttta tagtgatgga
     2281 accggtacta ttcttaacta tggaacgatt tgtgttaaca ccaattgcct gacgggcaat
     2341 gactataatg agactgactc ttatacctcg ttactttaca cgggtggcga tgtaatcact
     2401 gcgcagaatg aaacacaaag tctggctcaa aaagccacga ttaacgacaa aaaagaaggc
     2461 aatgttgtta acagtggttc gctttccggt gctgatattg cgattagtag cggtgaatta
     2521 gttaatacat cgacgggtat tattaataat gccatcatta tcaatgacgg agagttaagt
     2581 aacgaaggct ctgtggcgaa ggtgacgcta aacgcaggca cgttcgggaa catcggtacg
     2641 gttaactcgc gtatgttcca gacaggaggg acatttaata accagcaagg tggggtcgtg
     2701 caaaacggtg ctaacctgag taaaactgca atcgccaata acgcgggtac ttggtatctc
     2761 ggtgccagca gtagtagtga tagcaataat gccagcatga tggagattta taatactgcg
     2821 gtgtttaaca acagcggtga cttcattctg aataacagca gaaatgctgt tcacttatat
     2881 cagtcaggga cgttctacaa taccggtcat atgttaattt ctggtgctaa ctatagcggt
     2941 aacgcaatta attactggaa cgcgaataat aatggacggt tcattaatag tggtactgtt
     3001 gatgtaactg caaaagcgct agcgaccagc ggtgttgatg ccagtaccaa ccatgcatac
     3061 ttctggaacc aaaatagtgg tatagtcaat tttgataaag acagcggcgt tgctgtgaaa
     3121 ttcacccaca gtaattatgt tgcacagaat gatggcacca tgaatattag cggtaataac
     3181 gctattgcga tggaagggaa taaaaatgca cagttgatta acaacggcac gattaatctt
     3241 ggagcacaag gtacgacaga taccggtatg ataggtatgc agctggatag cagtgcgacg
     3301 gctgatgcgg tgatcgagaa taatggtact atcaacatat acgctaataa ttcctttgcg
     3361 tttagcatgc taggttccgt aggccacctc gtgaataacg gtacggtaac cattgcagat
     3421 ggggtgacgg gggccgggct gattaagcaa ggtaatagcg ttaacattga aggcgtcaat
     3481 ggcaataacg gcaataatag tgaagtgcat tatgctgatt acactttgcc tgatgtacct
     3541 gactcgtcgg tttctgttgc ggccagtaac ccgtccagtg acggaagcca gaacaaactg
     3601 aatggttatg ttgttggaac cagttctgac ggcagcgcgg gcaagctgaa agtaaacaat
     3661 gcaagcctga aaggggtttc tgttaacacc ggatttacgg ctggaaccag ctccacctcc
     3721 gtgaccttcg acaacgtcgt tcagggcagt aatctgaccg acgcagagac gattacatcg
     3781 acctctgtgg tatggaacgc acaaggcact actgacgcca gcggcaacgt tgatgtcacg
     3841 atgactaaaa atgcctacac cgatgtagca acagattcat ctgttaacac cgttgcgcag
     3901 gtactggatg cgggctatac caacaacgag ttgtacagca gcctgaacgt aggcaccacg
     3961 gcggaactga acagtgcgct gaaacaggtc agcggcagcc aggcaacaac ggtatttaat
     4021 gaagcacgag tgttaagtaa ccgcttcagc atgttgtccg atgcagcacc ggaagttgca
     4081 aacggtctgg ccttcaacgt ggtagcgaaa ggcgacccgc gtgctgaact gggtaacgac
     4141 acacagtacg acatgatggc gctgcgtaaa tctctgacgc tgactgaaca tcagaacctg
     4201 agtctggaat acggtattgc gcgtctggat ggtaatggtt cagataccgc aggcgataat
     4261 ggtgtgactg gcggttacag ccagttcttc ggtctgaaac accagatggc gtttgacaac
     4321 ggtatgagct ggaataacgc cctgcgttat gatgtgcata atctcgatag cagtcgctcg
     4381 atagcttacg gcgacatcaa taaaacagcc gatgctaatg tgaaacaaca gtacctggag
     4441 ttccgtagtg aaggagcaaa aacctttgag ctgcgtgaag gtctgaatgt gacgccttat
     4501 gcgggtgtga aactgcgtca tacgctggaa ggtggctatc aggaacgcaa tgcgggtgac
     4561 tttaacctga gcatgaacag cggcagcgaa acggcggtgg acagcatcgt tgggctgaaa
     4621 ctggactatg cagggaaaga aggctggagt gcgaacgcca cgctggaagg tgggccgaat
     4681 ctgagctatg tgaaaagtca acgtactgcg agcgtttcgg gcgcgggaag ccagcgtttc
     4741 aacattgatg atggtcagag tggcggtggt ttcaacagcc tggcgacgat gggtgtgaaa
     4801 tacagcagcc aggaaagtgc gttgcaggta gatgcattcc actggaaaga agacggcatc
     4861 agtgataaag gtgtgatgct aaacttcaag aaaacgttct aatttttagc atgtaataca
     4921 taaaccgcaa cgctgataca ggttgcggtt tttttattgc cggatgcggt gcttgacgtg
     4981 tcttaacctg tctggggggg cgtgtctttc agggcaacag tacaactcat cccgcaatct
     5041 tgtttacacc ctcgcattat ttatctctat tcatttctat actgattttt cttaatccgt
     5101 tttattacag ggcagggtgc gatgagcagc aacacattta ctctcggtac aaaatccgtt
     5161 aaccgtcttg gttatggcgc gatgcaactg gcaggtcctg gggtttttgg ccccccaaga
     5221 gatcgccacg tcgctataac cgtgctgcgc gaggcgctgg cattgggcgt caatcatatt
     5281 gataccagcg acttttatgg tccgcacgtc accaatcaga ttatccgcga agcgctttat
     5341 ccgtactctg acgacctgac aattgtcact aaaattggtg cgcggcgtgg agaggacgct
     5401 tcctggttac ccgcattttc tccggcagag ctgcaaaaag cggtacacga taatctacgt
     5461 aatctcggcc tggacgtact ggatgtggtt aacctgcgcg ttatgatggg ggatggtcat
     5521 ggcccgacgg aaggatcgat tgaggccagc ctgacagtgc tggcggagat gcaacaacaa
     5581 ggcctggtaa aacatattgg cctgagtaac gtcacaccga cgcaggttgc agaggcgcga
     5641 aagattgccg aaatcgtctg cgtgcagaac gaatacaaca ttgcccaccg tgctgatgac
     5701 gcaatgattg atgctttggc tcgtgatggc attgcctacg tgccattctt tccgctcggg
     5761 ggctttacgc cgctgcaatc ctccacgctt tccgatgttg ctacaagcct taatgcgaca
     5821 ccaatgcagg tggcgctggc gtggctgtta cagcgttcac cgaatatttt gctgattcct
     5881 ggcacctctt cggtggggca tctgcgggag aatatggcgt ccgatgaatt agtgctaacg
     5941 gatgacgtgt tggcaacgct ggacagtatt tcgcaaaagg cgggttaaat aaaaaaacct
     6001 cccactttcg tgggaggcat cataaatctt atgcagcaac aatgctgtcg atggctgctt
     6061 tcgcgtcaga ctgtgctttc gctgccattt ccgggccgta cgcgatccct tcggcgaaga
     6121 caaatttcac atcggtaatg ccgataaagc ccaggaatgt ggacagatat ggcgttacca
     6181 gatccgttgg gccatcttta tggatcccac cgcggctggt gataacgatg gcttttttac
     6241 cggtgaccag gccttccgga cccttctcgg tatagcggaa agtaacgcct gcacgtgcaa
     6301 ccaggtcaaa atagtttttc aactgggtcg agatattgaa gttatacatc ggtgccgcaa
     6361 taacgataac gtcgtgggct ttcagctcgg caatcagctc atcggaaagc gccagagctt
     6421 cctgctgacg cggagtcagc ggcgcatcgc tcggacgcag tgcaccaacc agttcgccat
     6481 ccaggaccgg aatcggattt gcagccaggt cacgcacggt gatttcatca gcggagtgct
     6541 tttcgcgcca ttgttcaaca aaataatcgg acaactgatt agactgagag taccctgcca
     6601 ggatgctgga tttaagaact aataccttgc tcatggtgtt tccttataga tatttgaatg
     6661 ggcaatgccc cgttgcttgt tgacacttta ttcacaattc tgccacagag atagcgcaat
     6721 aaatcgaagc ctatgttcga atttattgaa caacgcatag aaagccgcga tgtggtactc
     6781 tatatctatc atttaaaaga aaattaatca ggcagactac tgcccactaa cgttatgaca
     6841 gaacaacaaa aattgacctt tactgccttg cagcagcggc tggattcgat gatgctgcgt
     6901 gacagactgc gtttttctcg ccgtctgcac ggcgtgaaga aggttaaaaa tcctgatgca
     6961 caacaggcca ttttccagga gatggcgaaa gagattgacc atgcggcagg gaaagtcctg
     7021 ctgcgtgaag cggcacgacc ggagattact tacccggaca atttgccggt tagtcagaaa
     7081 aagcaggaca ttctcgaagc gattcgtgac caccaggtgg tgatcgtcgc cggggaaacg
     7141 ggttccggta aaacgactca gctaccgaaa atctgtatgg agctgggtcg cgggattaaa
     7201 gggctgatcg gccataccca gccgcgtcgg ctggcggcgc gaacggtggc gaaccgtatt
     7261 gcggaagagc taaaaacgga gccgggcggt tgcatcggtt acaaagtgcg tttcagcgat
     7321 cacgttagtg ataacacgat ggtcaagctg atgaccgacg gcatcctgct ggcagagatc
     7381 cagcaagacc gcctgctgat gcagtacgac accatcatta ttgacgaagc gcacgaacgc
     7441 agcctgaata tcgattttct tctcggctat ttgaaagagt tgctgccgcg gcgtcctgac
     7501 ctaaaaatta ttatcacttc cgcgactatc gacccggaac gcttttcgcg ccactttaat
     7561 aatgcgccga ttatcgaagt ctccggtcgg acctatccgg tggaagtgcg ctatcgcccg
     7621 attgttgaag aagccgatga caccgagcgc gaccagttgc aggcgatttt tgacgccgta
     7681 gatgaactga gccaggaaag ccctggcgac attctgatct ttatgagcgg tgagcgagaa
     7741 attcgcgata ccgccgatgc gctgaacaag ctgaacttac gccataccga aatcttgccg
     7801 ctttatgcac ggctttccaa cagcgaacag aaccgcgtgt tccagtcgca cagcgggcga
     7861 cgcattgtac tggcgaccaa cgtcgcggaa acctcgctga ccgtaccggg tattaaatac
     7921 gttatcgacc caggtacggc gcgtatcagc cgctacagct atcgcaccaa agtgcagcgt
     7981 ttgccgattg agccaatttc gcaggcttca gctaatcagc gtaaaggccg ctgcggccgt
     8041 gtgtcagaag ggatctgtat tcgtctctat tccgaagacg atttcctctc gcgcccggag
     8101 tttaccgacc cagaaattct gcgtactaac ctggcctcgg ttattttgca gatgaccgca
     8161 ctgggcttgg gcgatatcgc cgcattcccg tttgtcgaag caccggataa acgcaatatc
     8221 caggatggcg tgcgtctgct cgaagagctg ggcgcgatca ccactgatga acaggccagc
     8281 gcctataaac tgacgccgct cggtcgccag ctctcgcagt tgcctgtcga cccacgtctg
     8341 gcgcgtatgg tgctggaagc gcaaaaacat ggctgcgtgc gtgaggcgat gattatcacg
     8401 tccgcgctct ctattcagga cccgcgtgag cggccaatgg acaagcagca ggcatcggac
     8461 gaaaaacatc gtcgcttcca cgacaaagag tctgacttcc tcgcgtttgt aaatctgtgg
     8521 aattatcttg gcgagcagca aaaggcgctt tcttctaacg ccttccgtcg cctgtgtcgt
     8581 actgattatc tcaactatct gcgcgtgcgc gaatggcagg atatctacac ccagttgcgc
     8641 caggtggtga aagaacttgg cattccggtt aacagcgaac cggcggatta tcgcgaaatt
     8701 cacattgcgt tgctgaccgg tttgctttcg catatcggca tgaaagatgc cgataaacaa
     8761 gaatataccg gcgcacgtaa cgcgcgtttc tccatcttcc ccggttctgg tttattcaaa
     8821 aagccgccga aatgggtaat ggtggcggaa ctggtagaaa ccagccgcct gtgggggcgc
     8881 attgcggcac gcatcgatcc agaatgggtg gaacctgtcg ctcagcattt aatcaaacgc
     8941 acctacagcg aaccgcactg ggaacgggcg cagggcgcgg tgatggcaac ggaaaaagtc
     9001 accgtttacg ggttgccgat tgttgctgcg cgtaaggtca actacagcca gatcgatccg
     9061 gcgttatgtc gtgaactctt tattcgccac gcgctggtgg aaggtgactg gcagacgcgt
     9121 cacgcattct tccgtgaaaa cctgaaacta cgggccgaag tggaagaact ggaacacaaa
     9181 actcgtcgcc gcgatattct ggtggatgac gaaacgttgt ttgagttcta cgaccagcgc
     9241 atcagccacg atgtgatttc cgcacgccat ttcgacagtt ggtggaaaaa agtcagccgc
     9301 gaaacgcctg atttgctcaa ctttgaaaag agcatgttga tcaaagaagg ggcggaaaaa
     9361 atcagcaaac tggattaccc gaacttctgg catcagggca atctcaaact gcgtttgagc
     9421 tatcagtttg agcccggcgc ggatgctgac ggtgtgaccg tgcatatccc gctgccgcta
     9481 cttaaccagg ttgaggagag tgggtttgag tggcagatcc ccggtctgcg tcgtgagctg
     9541 atcatcgccc tgattaaatc gctgccgaag ccggtacgcc gtaattttgt gcccgcgcca
     9601 aactacgccg aagcgttttt aggccgcgtc acaccgctgg aattgccgtt gctcgacagc
     9661 cttgaacgag agttacggcg gatgactggc gtcaccgttg accgcgaaga ctggcactgg
     9721 gatcaggtgc ccgatcacct gaaaatcact ttccgcgtgg tggatgacaa aaacaagaag
     9781 ctaaaagaag ggcgctcatt acaggatctg aaagacgccc tgaaaggcaa agtgcaggaa
     9841 acgctgtctg ccgtggcgga tgacggtatc gagcagagcg ggctgcatat ctggagtttt
     9901 ggtcaacttc cggaaagtta cgagcagaag cgcggcaact acaaagtgaa ggcgtggccg
     9961 gcgctggtgg atgagcgtga cagcgtagcg atcaaactgt ttgataaccc gctggaacag
    10021 aagcaggcaa tgtggaacgg tcttcgccgt ttactgctgc taaatattcc atcgccgatc
    10081 aagtacttgc acgaaaagct gccaaacaaa gccaagctgg ggctgtactt taacccgtat
    10141 ggcaaagtgc tggagctgat cgatgactgt atctcctgcg gtgtggatca actgatcgac
    10201 gccaatggtg gcccggtctg gacggaagaa ggctttgctg cgctgcatga aaaagtccgt
    10261 gctgaactga acgacacggt ggtggatatt gcgaagcagg tcgaacaaat cctcactgcg
    10321 gtattcaata tcaataaacg cctgaaaggt cgcgttgata tgactatggc gctgggactg
    10381 tcggacatca aagcgcagat gggcgggctg gtgtatcgcg gttttgtcac tggcaacggt
    10441 tttaaacggt tgggcgacac gttacggtat ttgcaggcga ttgaaaaacg actggaaaaa
    10501 ctggcgattg atccgcatcg tgaccgtgct cagatgctga aagtcgaaaa cgtccagcag
    10561 gcatggcagc aatggatcaa caaactgccg cctgcacgtc gtgaggatga agacgtgaaa
    10621 gaggtccgct ggatgataga agagctgcgc gttagttact tcgctcaaca acttggcacg
    10681 ccttatccga tttcggataa gcgtattttg caggcgatgg agcagattag cggttaacac
    10741 tgctatttgc ctgataaatc taaaatccgg taagcattta gcgccgggtt ttttattaat
    10801 tctaaaaacg ataagggaaa aaattcagga gttcagaaaa atacaattct ctgctgcaag
    10861 atgaataatg tttatctaca gcatttcctt aaaaaatatg tcaggcttgc ggagtggcag
    10921 ttaaggaaat acgacttcct cctttcagag tgctccgctt ctcactatta tctcacgcag
    10981 tattcttaag ggaacgataa ggaggaacca tgaatattac cccgtttccg acgctttcgc
    11041 cggcaaccat agatgccata aatgttatcg gacagtggct ggcgcaggat gatttctccg
    11101 gtgaggtgcc gtatcaggcc gattgcgtga tccttgcagg caatgcggtt atgccgacta
    11161 tcgatgcggc atgtaagatt gcccgcgatc agcaaattcc tttactgatt agtggtggta
    11221 tcggtcactc gacaactttt ttgtatagcg ccatcgcaca gcatccacac tacaacacta
    11281 tccgcaccac tggcagagct gaagctacca tcctggcgga tatcgctcat cagttctggc
    11341 atattccgca tgaaaaaatc tggattgaag accagtcaac aaactgcggt gaaaacgcac
    11401 gctttagcat cgcgctattg aatcaggccg tagaacgagt tcatacggct atcgttgttc
    11461 aggaccccac catgcagcgg cgcacgatgg cgacgttccg ccgtataacc ggggataatc
    11521 ccgatgcacc acgctggtta agttatcccg gattcgttcc tcagttagga aataacgcag
    11581 acagtgtaat ctttgttaat ccgttgcaag gattatggcc ggttgagcgt tatctatcgc
    11641 tactcactgg cgagctgccg cgtttacgcg atgatagcga tggctacggc ccccgcgggc
    11701 gagattttat cgttcacgtt gattttccgg cagaagtcat ccaggcatgg caaacgctga
    11761 aacacgatgc ggtgctcatc gaggcgatgg aaagtcgctc gttacgttaa aaattgcccg
    11821 ttcgtgaacc aattgtttgc aaacgggcat gacttctgac ttttatttct gcctttaatt
    11881 ccttttacac ttgtttttat gaagcccttc acagaattgt cctttcacga tgccgtctct
    11941 ctgataattg atgttaatta acaataaatt taccaaaaac aaacatacaa atcacaggag
    12001 tcgcccatgt cagtacccgt tcaacatcct atgtatatcg atggacagtt tgttacctgg
    12061 cgtggagacg catggattga tgtggtaaac cctgctacag aggctgtcat ttcccgcatt
    12121 cccgatggtc aggccgagga tgcccgtaag gcaatcgatg cagcagaacg tgcacaacca
    12181 gaatgggaag cgttgcctgc tattgaacgc gccagttggt tgcgcaaaat ctccgccggg
    12241 atccgggaac gtgccagtga aatcagtgcg ctgattgtcg aagaaggggg caagatccag
    12301 cagttggctg aagtcgaagt ggcttttact gcggactata tcgattatat ggcggagtgg
    12361 gcacggcgtt acgagggcga gattattcaa agcgatcgtc caggagaaaa tatccttctg
    12421 tttaaacgtg cgcttggcgt gactaccggc attctgccgt ggaactttcc gttcttcctc
    12481 attgcccgca aaatggctcc cgctcttttg accggtaata ccatcgtcat taaacccagt
    12541 gaatttacgc caaacaatgc gattgcattc gccaaaatcg ttgatgaaat aggccttccg
    12601 cgcggcgtgt ttaaccttgt actcgggcgt ggtgaaaccg ttgggcaaga actggcgggt
    12661 aacccaaagg tcgcaatggt cagtatgaca ggcagcgtct ctgcaggtga aaagattatg
    12721 gcgactgcgg cgaaaaatat caccaaagtg tgcctggaat tggggggtaa agcaccggct
    12781 atcgtaatgg acgatgccga tcttgaactg gcagtcaaag ctatcgttga ttcacgcgtc
    12841 attaatagtg ggcaagtgtg taactgtgca gaacgtgttt atgtacagaa aagcatttat
    12901 gatcagttcg tcaatcggct gggtgaagcg atgcaggcgg ttcaatttgg taaccccgct
    12961 gaacgcaacg acattgcgat gggaccgttg attaatgccg cggcgctgga aagggttgag
    13021 caaaaagtgg cgcgcgcagt agaagaaggg gcgagagtgg cgttgggtgg caaagcggta
    13081 gaggggaaag gatattatta tccgccgaca ttgctgctgg atgttcgcca ggaaatgtcg
    13141 attatgcatg aggaaacctt tggcccggtt ctgccggtgg tcgcatttga cacgctggaa
    13201 gaggctatct caatggctaa tgacagtgat tacggcctga cctcatcaat ctatacccaa
    13261 aatctgaacg tcgcgatgaa agccattaaa gggctgaagt ttggtgaaac ttacatcaac
    13321 cgtgaaaact tcgaagctat gcaaggcttc cacgctggat ggcgtaaatc cggtattggc
    13381 ggcgcagatg gtaaacatgg cttgcatgaa tatctgcaga cccaggtggt ttatttacag
    13441 tcgtaatgag tgaaagaggc ggaggttttt tcctccgcct gtgcgcgtca gagtttagcg
    13501 aatttttcga gggtgcgaat aagctgcgtg acgaagccat attcgttatc gtaccaagcg
    13561 accgttttca ccagttgtaa atcgcccacg gcggtaattt ccgtttgcgt ggcatcaaac
    13621 accgaaccga aatggctgcc aatgatatcg gaagagacta tttcttcatc agtataacca
    13681 aatgactcgt tattgtttgt cgccttttta agcgcgttgt tcacctcttc ggcagtcact
    13741 ttctttccga gaatcgacac cagttcagtg accgaacctg ttttcaccgg cacgcgttgc
    13801 gcatgacctt tcagtttgcc gctcagttcc gggatcacca gaccaatggc ttttgccgcc
    13861 cccgtagtgt ggggaatgat attttctgcc gctgcgcgtg aagcacgtaa atctttaccg
    13921 cgcgggccat ccaccagtga ctgggtgccg gtataggcat gaatggtcgt catcgtgccg
    13981 acttctatcc cgaaactgtc atgcaaggct ttggccatcg gcgcaaggca gttagtggtg
    14041 catgacgcca cggaaacaat ggtgtcgttg ccatccagag tgtcgtcatt gacgttataa
    14101 acgatggttt tcatttcacc ggcaggggcg gaaatcaaca ccttcttcgc gccagcatca
    14161 agatgcgcct gcgatttctc ggcggaggta taaaagcctg tacattcgac aatgatttct
    14221 gcgcctttcg ctttccacgg aatattttta gcctcttttt cggcgtaaac cgcgatactt
    14281 ttcccatcaa cgataagtga atcttccgta taatcaacgc tccaggggaa cggtccgtag
    14341 tttgaatcat gtttcagcag gtaggcgaga atttttgggg aagtgagatc attaatagcg
    14401 acaacgtcta tgttgctttt gacttcaagt aatcgacgta acaccagtcg accgatacga
    14461 ccaaaaccat taataccaac tttactcatg gtcttctcct gtcaggaacg ttcggatgaa
    14521 aattgatcct ttccaggctt agaccaggat ggcgggatgg gcaatctaca ttcttgcagc
    14581 gaaacgtaac gtactgaaaa cgggtgaaca atatttaatg aaattttaag aaaagcccgt
    14641 tatgttaacg gcaaattatg ttaaagcagg aaatgttatg ggaaataaat actcaaagtt
    14701 acaaatcagc attcactggc tggtcttttt gctggttatc gtagcgtatt gcgcaatgga
    14761 gtttcgtggt ttcttcccac gtagcgatcg gcccctcatc aacatgattc atgtttcctg
    14821 tggtatctca attctcgtac tgatggtggt tcgtcttttg ttaaggctga aatgcccaac
    14881 cccgccgatt atacctaagc caaaaccgat gatgacggga ctggcgcatt tgggccattt
    14941 ggtgatttat ctcctgttta ttgcgctgcc agtgattggt ttggtgatga tgtataaccg
    15001 gggcaacccg tggtttgcgt ttggtttgac gatgccttac gcttcagagg ccaatttcga
    15061 acgggttgat agcttaaagt cgtggcatga aacgctggcg aatctgggat attttgtcat
    15121 cgggttgcac gctgcggctg cactggcaca ccactatttc tggaaggaca acacacttct
    15181 acgcatgatg ccacgtaaac gttcctgaag gatatttaaa gaaaacgcct gtactaaaac
    15241 cgacccgtgg tacaggcgaa gaatacgggc ctacatcgga agcgcctatt atatttattt
    15301 gtatgacaaa taaaaccata atccttgtct atacgtccat ctggcttatc tttaatcaat
    15361 tcacccgatc tttgatctca tcaacggtat caaaataact cagcgtataa cgttcaaatc
    15421 gaacataaga ttcgataaat catggaggtt atatgaaaaa actggcactt atcctggtta
    15481 tgggaacgct tgtttccttt tatgccgatg ccgggcgcaa accctgttct ggttcgaaag
    15541 gggggatctc acactgtacg gcaggcggca aatttgtttg taatgatggt tctattagtg
    15601 catcgaaaaa aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatccg
    15661 ctagtcagat gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga
    15721 gcgcagcaac ctccttatca ccgtcccgga accgcagttc gtagagcgat tgtcgggtca
    15781 ggagtgtgaa tgtcagaatc gtaatgcaga taatgagcag acacaccacc agagggttgc
    15841 gcttcatagc ctttctcctt gccggatggc gggtaagagg ctaagatctg aattgctagg
    15901 ttcattcgtt ggcctcggtt gatagaaata tcggtcgggg ccttcgtctt tctgattccc
    15961 ggttagcctg aaaacagaaa gtctcaggca cccgtgagca tcctatgtgg tttccttagg
    16021 gacgaaaata atcacctgac gaaattgcgt gctgttttcc agaatttttc gtcattcggt
    16081 ttagccagtt tagccattcg ttactctcgc aaggccgtgg atttgccgtt gtcgccagcg
    16141 aagtacggac actcgcaagc cgcagcgccc agacggcgaa agagattgaa ggcttgatca
    16201 gtgaatcagt caggttaatt gacctgggat cggatgaggt ggcaacggca gggaaaacca
    16261 tgtgcactat tgttgatgcc gtcgcgagtg tcacacatat catgcaggaa atcgccaccg
    16321 cctcgaatga acaaagtcga ggcataacgc aggttagcca ggcgatttct gaaatggata
    16381 aggtgacgca acagaatgct tctctggtag aagaggcctc aacggcagcg gtgtcccttg
    16441 aagaacaggc ggcacgatta actgaggcgg tggacgtatt ccgtctgaac aaacattctg
    16501 tgtcggcaga acctcgcgga gctggtgaac cagttagttt tgctacggtg tgaaaatgtt
    16561 aaaggaggga tcgacagatc ccttcacctt tcagaacggc attgattttc gaatagcgtt
    16621 aatcatcaac tggcaaccag aagagaacgt cgcatctacg cgggtcagta ttccaatcgg
    16681 ttcgcctgca ccatgtcccg gaacaggcag ggctaccagc gtggcatgac gcaggtcgtc
    16741 ttttacagcg ccagaaggga caaaccacac gtaatcgtat tcaaccgtaa gttgacgaga
    16801 tagcgaagca gacagcgttt cgatacaacc cgaaggaatt ttacatccct ggctctgcac
    16861 taatgcatct gaatgctggc gtggcgcagt gccttctggt gatacaacga ccggccattc
    16921 cagcacccgg cttagcgtta cgttctcctg aagtagcggg tgattagggc ggacaaccag
    16981 cttcagcgat tcaagaaaca gcagttcgta attaagcccg ttcatcagtt caggatctga
    17041 catccgacca atgccgatat cgatttcccc ggtttttaaa cccgccagaa tcatagggtt
    17101 actcattgtc gcaacttgca aggtcgtctc tttttgttgc tgatgaaact gacctataac
    17161 cgaaggtaat atccctagtg ccgcagtagg tagcgcacca accctgacga catcattatt
    17221 aagaccttct ttacgatgaa gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac
    17281 tgcatgcgtt aaaaattgtt cgccgggtaa ggtaagttgc gccccctgac gaccacgctc
    17341 aaacaagcga gcgcccgtca gctgctccag ttcattcaat gtcttagaga gcgcaggttg
    17401 actcaaatta agggtttcag ccgcgcgccc caaagttcct tgttgtgcga cagctacgaa
    17461 tgtatgaagg tggcgcaaac ggatgcgctg actaaacaga ctattttttt ccataagcga
    17521 tgttaaaaac gaagcggtgt cgctgacaag tgaagttgtt ttattatgat aacttgattg
    17581 caaaatatta ttaacaatta aagcaactat gttacagcaa aatggataat attgatgttt
    17641 tcgcggcgag atcacagttt gtaaattctt ccagccggag tgaatgcggt tacctacact
    17701 ccagattact gaccactgga ggcagacact atggcgaaca gcatcacggc ggatgagatt
    17761 cgggaacagt tttcgcaggc aatgtcagcc atgtaccagc aagaagttcc gcagtacggc
    17821 acgctgctgg aactggtagc tgatgtaaat ctggcggtgc tggaaaacaa tcctcaactg
    17881 cacgaaaaaa tggtaaatgc agacgagctg gcgcgactga atgttgaacg tcatggggcg
    17941 attcgcgttg ggactgcaca agagcttgct actcttcggc ggatgtttgc cattatgggg
    18001 atgtacccgg tgagctatta cgatctctcg caggcagggg tgccggtaca ttcgacagca
    18061 tttcggccca ttgatgatgc ttctctggcg cgtaatccct tccgcgtttt tacctcgtta
    18121 ctccgtcttg agcttatcga gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt
    18181 cagcgcgata tcttcacccc acgttgtcga caactgttag aggaatatga gcagcagggc
    18241 ggttttaacg aaacacaggc acaggagttt gtgcaggaag ccctggaaac gtttcgctgg
    18301 caccagtcag caacggtaga tgaagaaacc tatcgcgcct tgcacaacga acatcggttg
    18361 attgctgatg tggtctgttt tcctggatgc catatcaacc atctgacgcc acgtacgctg
    18421 gatattgacc gggtgcagtc gatgatgcct gaatgcggaa ttgaacccaa agttctgatc
    18481 gaagggccgc cgcgccgcga ggtgccgatt ttactacgcc agaccagctt taaagcactg
    18541 gaagagacgg tgttgtttgc ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa
    18601 attgagcagc gtggtgtggc attaacgccg aaagggcgac aactgtatga tgatcttctg
    18661 cggaacgctg gaaccgggca ggataatctc actcaccaaa tgcatttaca ggaaaccttc
    18721 cgcacttttc ctgacagtga gtttttaatg cgtcagcaag ggctgggatg gttccggtac
    18781 cgtctgacgc cttcgggtga ggcgcatcgt caggtgattc atcccggaga cgatccacag
    18841 cccttaatag aacgtggttg ggtagtggcg caacccatta cctatgaaga tttcttgccc
    18901 gttagcgcgg cggggatctt ccagtcaaat ctgggtaatg aaacgcgggc atgcaatcac
    18961 ggtgatgcca gtcgcgaagc atttgagcag gcgttggggt gtccggtttt ggatgagttc
    19021 cagctttatc aggaagcgga agaacgcagt aaacgtcgct gtggtttgct ttaaattctg
    19081 accatccgcc tttgcaaaaa tttgcctgat tttacaaacg aatcaggctc atcccatcga
    19141 cataaaaaaa atg
//
LOCUS       DADTFG010000093        18756 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.093, whole genome shotgun sequence.
ACCESSION   DADTFG010000093 DADTFG010000000
VERSION     DADTFG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.093"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            86..1057
                     /locus_tag="J1138_003020"
     CDS             86..1057
                     /locus_tag="J1138_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HBA8454788.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISNGARVTAQDVPITGGPYKNIVANYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQQQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1160..1663
                     /locus_tag="J1138_003021"
     CDS             1160..1663
                     /locus_tag="J1138_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBA8454789.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1730..3187)
                     /gene="nuoN"
                     /locus_tag="J1138_003022"
     CDS             complement(1730..3187)
                     /gene="nuoN"
                     /locus_tag="J1138_003022"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBA8454790.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3194..4723)
                     /gene="nuoM"
                     /locus_tag="J1138_003023"
     CDS             complement(3194..4723)
                     /gene="nuoM"
                     /locus_tag="J1138_003023"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBA8454791.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4887..6728)
                     /gene="nuoL"
                     /locus_tag="J1138_003024"
     CDS             complement(4887..6728)
                     /gene="nuoL"
                     /locus_tag="J1138_003024"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBA8454792.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNITAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6725..7027)
                     /gene="nuoK"
                     /locus_tag="J1138_003025"
     CDS             complement(6725..7027)
                     /gene="nuoK"
                     /locus_tag="J1138_003025"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBA8454793.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7024..7578)
                     /gene="nuoJ"
                     /locus_tag="J1138_003026"
     CDS             complement(7024..7578)
                     /gene="nuoJ"
                     /locus_tag="J1138_003026"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBA8454794.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7590..8132)
                     /gene="nuoI"
                     /locus_tag="J1138_003027"
     CDS             complement(7590..8132)
                     /gene="nuoI"
                     /locus_tag="J1138_003027"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBA8454795.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8147..9124)
                     /gene="nuoH"
                     /locus_tag="J1138_003028"
     CDS             complement(8147..9124)
                     /gene="nuoH"
                     /locus_tag="J1138_003028"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBA8454796.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9121..11847)
                     /gene="nuoG"
                     /locus_tag="J1138_003029"
     CDS             complement(9121..11847)
                     /gene="nuoG"
                     /locus_tag="J1138_003029"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBA8454797.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIIGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSQTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11900..13237)
                     /gene="nuoF"
                     /locus_tag="J1138_003030"
     CDS             complement(11900..13237)
                     /gene="nuoF"
                     /locus_tag="J1138_003030"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBA8454798.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13234..13734)
                     /gene="nuoE"
                     /locus_tag="J1138_003031"
     CDS             complement(13234..13734)
                     /gene="nuoE"
                     /locus_tag="J1138_003031"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBA8454799.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13737..15539)
                     /gene="nuoC"
                     /locus_tag="J1138_003032"
     CDS             complement(13737..15539)
                     /gene="nuoC"
                     /locus_tag="J1138_003032"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBA8454800.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGIIFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15633..16295)
                     /locus_tag="J1138_003033"
     CDS             complement(15633..16295)
                     /locus_tag="J1138_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HBA8454801.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16311..16754)
                     /gene="nuoA"
                     /locus_tag="J1138_003034"
     CDS             complement(16311..16754)
                     /gene="nuoA"
                     /locus_tag="J1138_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBA8454802.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17384..18322)
                     /gene="lrhA"
                     /locus_tag="J1138_003035"
     CDS             complement(17384..18322)
                     /gene="lrhA"
                     /locus_tag="J1138_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBA8454803.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4716 a         5166 c         4819 g         4055 t
ORIGIN      
        1 ataatcctgc taatcttaat aaaatacaat atcgaaaccc ataacaaata atgattcaac
       61 aacgcaaccc attaataatt tgcatatgaa aaaaataaat tttgctttta ttatattatt
      121 tctgttttca ctccctctga ttatttttta ccaaccctgg gtgaatgcat taccgccaac
      181 gccccgacac gcaagtcctg agcaattaga aaaaacagtt cgttatctta cacaaacagt
      241 gcatccacgt agcgccgaca atattgataa tctgaatagg tccgcagagt atataaaaga
      301 ggtctttatc agtaacggtg ccagagttac cgcgcaggat gtccccatta cgggtggccc
      361 ctacaaaaac attgttgcta attatggtcc tgccgatgga ccgctgatta ttattggtgc
      421 gcactatgac tctgtcagta gttatgaaaa cgatcaattg acctatacgc cgggcgcgga
      481 tgataacgcc agcggcgtgg caggattact cgaactggca cgtttgttac agcagcagac
      541 tccagggatt ggcgtccaac tggtcgccta tgcgtcggaa gaaccgccct tctttcgtag
      601 cgatgaaatg gggagcgcgg tacatgcagc ttcgcttgag cgtccagtga aattaatgat
      661 agcgctggag atgattggtt attacgactc aacgcctggt agccaggatt acccatcccc
      721 ggcaatgtcc tggctttatc ccgatcgggg agactttatt gccgtggtcg gcagaatgca
      781 ggatatcaac gccgttcgtc aggtaaaagc ggcattgttg tcatcccggg atttatctgt
      841 ttattctatg aatgccccag gttttattcc cggtattgat ttctctgatc accttaatta
      901 ttggcaacac gatattcccg ccgtaatgat tactgacacc gctttttatc gtaataaaca
      961 ataccacttg cccggtgata tcgcagacag attaaattat cagaaaatgg ctcaggtagt
     1021 ggatggagtt acaaccttgt tatacaacag taaataataa aaatttctcg aggcggatcc
     1081 agaaaaagca acatgcgcat tttattttct ttcgtctgtt ttaagatgcg aaacatgaaa
     1141 ttcaacggag gaaaaataaa tgggaatgat tggctatttt gcagaaattg actcagaaaa
     1201 gattaaccaa ctgcttgagt ctactaaaaa acctttgatg gataatatac atgacacgct
     1261 ttcaggatta cgccgactgg atattgataa acgctgggat tttttacact ttggcctgac
     1321 gggtacatct gcatttgacc ccgcgaagaa tgacccgcta agtcgcgcag tgcttggtga
     1381 acacagccta gaagatggca tagatggctt cctgggattg acctggaatc aggagttagc
     1441 cgcgacaatc gaccgactgg aatcactgga tcgaagtgag ctgcgtaaac agttttccat
     1501 taagcgactc aatgaaatgg agatttatcc gggagtaacc ttttcagaag agttagaggg
     1561 gcaactcttt gcttcgatca tgcttgatat ggaaaagctg attagcgcgt atcgacgtat
     1621 gctgcgacaa gggaatcatg cgctgacggt tattgttggg tgatagtgtc agggcaccaa
     1681 aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa actgactgct tacatcagcg
     1741 gcattgctaa ccgcacaatg ctaatcagtg gttgtggcca tacacccagc accagtacca
     1801 acagtgcaga gatcagcacc acgataccgc ctgcgctgta ctgccagttt gatggtgcat
     1861 cgcgacccgg ttgttccgga gcgtgaaggt ataagctcac cgccacgcgc aggtagtagt
     1921 agaggccgat tgccgaacca acaaccacgg cacccaccag ccaccacaag tgtgcctgga
     1981 caccgaccgc cagcacgtag aacttaccga taaagcccag cgtcattggg ataccagcca
     2041 gtgaaagcat catcaccgtc atcactgccg cgaggatcgg acgatgccag aacagaccgc
     2101 ggtaagagaa cagggaatca gcatccgggc cacgatacgg gctggacatc aggctgacca
     2161 cgccgaacgc gccgaggctg ctgaacagat aaccgaccag gtaaacccct accgcttcca
     2221 tcgacatctc gccggtttgc agcgcaatca gcgccaccag cagatagccg aggtgagaga
     2281 tagatgagta accgagcaga cgtttgatat tggtctggct caacgccatc aggttaccga
     2341 agatgatgga ggcaaaggcg ataatcgcca gcactacgcg aatcgcttca ctgtcaccca
     2401 ccggtgcgta aaggaacaaa cgcatcacca cgccgaagat agcgattttg ctcgccgtcg
     2461 ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga
     2521 acggcaccag agagagtttg aaaccgaggc caacaatcat caggccaaaa cctgccagca
     2581 acagcggctc gttgagcata ccgtcgccga ggtttttacc cagtgcgaca aacgacaggt
     2641 cgccagactg cgcatacacc agcgccatac cgaacagcag gaaagaagac gctgcggcag
     2701 aaaggatggt gtatttgata ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga
     2761 ccaggccaaa cagcggcaaa gagatcagtt cgataccgag gaacagagac gccagatggt
     2821 tggcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg tagaactcgt
     2881 ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg
     2941 ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc agcggcgtaa
     3001 cgtccatagc gcccgcctgg ccgacaaacc agagcgaaac cagcgccgcg ttaagcccaa
     3061 taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa
     3121 ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt tgtggagtta
     3181 ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac tgctggatat
     3241 tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg atagaagccc agcagtacca
     3301 gcagcaccac cagcaacagg atcataaaca gctcacgcag cgacatccct ggcagttcct
     3361 ggctggcaat ctggcttttc gctttgccga agtaagcgcg atgcagcatc gccagcgaat
     3421 aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg acaacctgga
     3481 agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgccaggc atcccaagcg
     3541 ttgccaccgc aaagaacagc gacagtgctg gcagccattt cattttgctc cacagaccgc
     3601 ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccacaa agaataaaca
     3661 gacccgccgc cgacaagccg tgcgcaatca tctggattac cgcgccctgg taggccaact
     3721 ggctgccggt gtagatagca atcagcacga agcccatgtg ggaaaccgag gtgtaggcga
     3781 tcagacgttt gatatcggtc tgggcgaagg ccatccacgc accatagaag atgccgataa
     3841 cacccagcca catagcgatt ggcgcaaact ctgccgacgc gttcgggaac agcggcaagg
     3901 agaaacgcaa cagaccgtaa gcggcggttt tcagcaagat ccccgcgagg tcaacggaac
     3961 cggcggtcgg tgcctgggag tgcgcatccg gtaaccagcc atgcagcgga accaccggca
     4021 ttttcaccgc gaaggcgatg aagaagccca gcatcaacag atattccacg ccgttagaca
     4081 tcggcgtatt cagcagctct tcatagttga aggtccagac gccggtcgca ttgtagtgaa
     4141 cgaaaaccag cgccaggatg gcgatcaaca tcaccagacc actcgcctgg gtgtaaatga
     4201 agaacttggt tgccgccgtg atacgcgttt taccgtcaga ggctttatgc ccccacagtg
     4261 cgatcaggaa gtacatcggc accagcatca tttcccagaa gaagaagaac aggaacatgt
     4321 cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg aggtggaaga
     4381 agccctggta tttttcgatc tctttccacg aacagagtac cgccagcaca ccgagcagac
     4441 cggtcagcac gaccatcagc aacgacagac cgtcaatggc gagatggata gagataccaa
     4501 aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg gcggattgcg
     4561 tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc aatcccatgg
     4621 ttaccagcgc gatccagcgc ggcaccttga cgccaaagcg ttcggtctgc cagcacagga
     4681 agccgccgat aaagggaatt aatattagcc agggtagtaa catggcgatc tttattcctt
     4741 gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca cgacagaaaa ttctcttcgg
     4801 actgggggta gtgtcggatg cgccgcgaag acggcttatc cgacctacgt tcttatatgc
     4861 ccaggggcaa tcccacaatc cttaactcaa cgcagtacca tcaacagtgc cagcaccacg
     4921 accgcaccga tgctcatgga tgccacatac cagcgcagat aaccgttctc gcttaacagc
     4981 agacctttac ctgcaaagcg ggaaaggaca gccgtgatgt tcatcattga gttcagcgga
     5041 tcgcgtttca gcaaccaggc aatacccagg aacggcttga cgaacacttt gtcgtacagc
     5101 cagtcaaagc cccaggcgtt gtaccaccag gtacccagca gacggcccgg cgcactgttg
     5161 gcgatggagg tcaccagagt acgtttaccc agccacagcc aggcagccag cagaatgccg
     5221 accaccgcga ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg cgccagttcc
     5281 gtcgtttgcg gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac gaaggtggaa
     5341 aggatcagca gcacaatcag cggcaggctg tgagttaccc ctttcacggc gtgagcgtga
     5401 atttgttctt ttccgtggaa gacgatgaaa atcatacgga aggtgtaaag cgaggtcata
     5461 aatgcaccga ccagacctgc caccatcaga ttgatatgtc cattcgccat cgcacccgcg
     5521 aggatctcat ccttactgaa gaagcccgca gtgaccagcg gcagtgccga cagtgctgcg
     5581 ccgcccacca ggaagcagag ataaaccagc ggaatagatt tacgcagacc acccatctta
     5641 aagatgttct gttcgtgatg gcaggccaga atgacggaac cggatgccag gaacagcagc
     5701 gctttaaaga acgcgtgggt catcaagtgg aaaatcgccg catcccatgc ctgcacgcca
     5761 agcgcgagga acatgtagcc aatctggctc atggtagagt aagcgagaac acgtttgatg
     5821 tcggtctgta ccagcgccgc aaaaccggcc agcagcagcg taaccgcccc aacaatgccc
     5881 accagatgca gaacttccgg cgtcatcagg aacaggccgt gggtacgggc gatcaggtag
     5941 acacccgcgg ttaccattgt tgcagcgtgg atcagcgcgg agacaggcgt cgggcccgcc
     6001 attgcgtcgg caagccatgt ctgcaacggc aactgagcag atttaccgac cgcaccgccc
     6061 agcagcatca gcgtcgccca catcagcatg ttattgccgt cagcaaagtg cgctggtgcc
     6121 agttccacca tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat gaacagcgcg
     6181 aaagcgagga agacgtcacc cacacgggtc acgacgaacg ctttcattgc cgctgcgcca
     6241 ttcttcggat cggtgtaata gaaaccgatc agcagatagg agcacaggcc cacgccttcc
     6301 cagccgaggt acatcagcag caggttgtcg gcaagcacca gaactaccat gctggcgatg
     6361 aacaggttgg tgtaagcgaa gaagcgagag tagccctctt caccgcgcat ataccaggag
     6421 gcgtacatgt gaataaggaa acccacacca gtgaccaccg agagcatggt cagcgacagg
     6481 ccgtccagca ccaggttaaa accgatgtta aagtcgccta ccgacatcca cgtccacagc
     6541 ggctggctgt atgcctgctc accgttagcg aagaagtcaa cgccgataaa ggcggttacc
     6601 agcgccgcca ggcccacaga gccaacgccg acgatcgccg agacgttttc agaccagcgc
     6661 ccacgggaga acgccagcag gacgaagcca atcaatggca aaataatggt taaggcaagc
     6721 atgttcatcc gcgcatctca cttactgaat cgatgttcag gttctggcga cgacggtgaa
     6781 gttgcagcag cagcgcaagg ccgatactcg cttccgccgc cgcgaggctg atggcgagaa
     6841 tgtacatcac ctgaccgtcg gtctggcccc agtagcttcc ggcgaccaca aaggccaacg
     6901 cagaggcgtt aatcatgatt tccagaccaa tcagcataaa cagcagattg cgacggataa
     6961 ccagaccggt taagccaaga acgaataaga ttgccgcgag gatcagtcca tgttgtaagg
     7021 ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg tctttacgat tgctcagcac
     7081 ttcacccgca cgctcttcac gaccgacgtg gaaggccaca accagacccg cgagcagcag
     7141 catagaagcc agttccaccg ccagtacgta aggcccgaac agcgtaatac ccactgcttt
     7201 agcactgatt ggcgtaccgt cgataccttg atcgttaaca ccgagaatgg cgtaaacaat
     7261 caccaccagc atgatggctg acaaaattgc cggaccaatc cacacctgcg gtttcagcca
     7321 ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc atcatcacca cgaacacgaa
     7381 cagcaccata atggcacccg catagacgat aatttccagc gcaccggcga agtaagcgcc
     7441 cagtgagaag aacaccccag agatcgccag cagcgaaata atcaggtaca gcagcgcgtg
     7501 taccggattg gtatgggtga tcactcgcaa ggtcgcaagt atggctatca ggccacagat
     7561 ataaaaagcg aactccattg cccctctcct tacggtaaca ggctcttgac gtcgataggc
     7621 ttggcttcgt tctctgcttc gcccttatct ttgccgtcga ttgccatacc tgccatccgg
     7681 tagaagttat attccgggta tttgcccgga ccggagatca gcagatcctc tttctcgtaa
     7741 accagatcct ggcgcttgta ttcccccatt tcgaaatccg gggttaactg aatcgccgtg
     7801 gtcggacagg cttcttcgca cagaccacag aaaatgcagc gtgagaagtt gatgcggaaa
     7861 aattccggat accagcgacc gtctttggtt tctgcttttt gcagcgagat acagccgacc
     7921 gggcaggcta ccgcgcagag gttacaggct acgcaacgct cttcgccgtc cggatcgcgg
     7981 gtcagaacga tacgaccacg ataacggggc ggcagataga ccggctcttc cgggtacatt
     8041 cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc agatactacg aacctgggtg
     8101 ccgaaaccta ctaacaattc ttttaaggtc atggtcttat tgccccttat tgcgcctgcc
     8161 agagaatgac agccgccgtt accagcaagt tgatcagcgt cagcggcagg cagattttcc
     8221 agccgaagga cattacctgg tcataacgcg gacgcggtaa cgacgcacga atcaaaatga
     8281 acatcatcat aaagaacgcg gttttcagcg cgaaccagat gaatggcggt aacaacgggc
     8341 cttgccagcc accgaagaac agcgtcacca tcagtgcaga gatggtcaca atcccgatgt
     8401 attcgcccac gaagaacaga ccgaacttca taccggaata ttcaatgtgg taaccatccg
     8461 ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg gtgacacacc gccacgcccg
     8521 cgatggcaaa ggtaataaaa ccaaagaatt gcgggatgac gttccacaca tgcgcctggc
     8581 tattgacgat gtcggtcatg ttgaatgaac cggcctgcgc caccacgccc atcagggaaa
     8641 gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc acgcatcgca cccagcagcg
     8701 agtatttgtt gttacttgac cagcccgcaa acagcaccgc gtaaaccgcc agacctgcca
     8761 tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac cacccaaccc ggactgactg
     8821 gcacaattgc aaaggccagc agcagcgagg taaaggcaat catcggtgcc agggtaaaga
     8881 tgacgcgatc cgagaatttc gggatccagt cttctttaaa gaacattttg atcatgtccg
     8941 caaccagctg gagcgaaccg ccccagccaa cacggttagg tccataacgg ttctggaaca
     9001 gacccagcag gcgacgttcg ccaaagctca tgaatgcccc acaggtgact accaccagca
     9061 ggatcaccac cgctttaagg acggtcagca ggatctcaat cagttccggt gatatccaac
     9121 tcattgttgt gcctccttga gatcctcaag atgcgcgcca gccagcaccg gagcaatgcc
     9181 ggacataccc atcggcaagc ccacctgccc tgctgttagc ccttcggcga tttcaaccgg
     9241 cagcgtgacc gtgttgccat cgtaactaaa ggaaacgcgt gtacctgcgt tcacacccaa
     9301 cttcgcggca tccgctgggt tgagtttgat gtacggctgc ggcatacggc tctggaagac
     9361 cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg taatacggcg cgatacgcca
     9421 tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg aagtaatcca gaccattttc
     9481 gctggtttca aacagacgca cgcccggatc gccaaagcgc agtttgccgc ccacttcgtc
     9541 ctggaatttg ttccacgcct gcggggagtt ccagcctggc gcccaggcaa acggcacttg
     9601 cgaacggtgc gcggtcggct ggttgttacc ttccatcgag aaggtgaaca tggtgtcaat
     9661 atcctgcggc tgacgcggct catgaacgct gatattggcg cgcatggcgg tacggccgct
     9721 atagcgatgc ggttcacggg ccagtttctg cccacgaata cggaatgtcg catccggcgc
     9781 agcatctttg atacctgcca gttccgggat tttcgccaca acagcgtcaa tcacgtggtc
     9841 gagctgcgtc cagtccactt cacggctcag cagggtgcta tgcagcgagt gcagccagcg
     9901 ccagctttcc agcatgacag tttggctgtc gtaataagcc ggatcgtaaa cctggaagaa
     9961 acgttgggcg cggccttcgt tgttgatcac cgtaccgtcg ctttcagcaa agctggcggc
    10021 ggaaagtacc agatgagcgt tttccataat cgctgtgcgt tgatggtcaa ccaccatcac
    10081 cagcggcgct ttagccagcg cagcattcac gcgggtagcg gaagcgtgac gatgcagatc
    10141 gttttccagc accaccaccg cgtcggcgcg tccggtttcc agttcagtta acgcttcttc
    10201 aagcgatcca ccgcctataa tgcccagccc catgctgttg acggaacggg caatcatggt
    10261 gataccgacg tcagcgccgc gacctttcag ggctttcgcc acgttagccg cagcctgaat
    10321 cacttcagcg ctaccagcgt tcgtcccgga gataatcaac ggtttcttcg caccagccag
    10381 tgcctgcacg atgacgtcga ttttgctttg cagctcaggt tcgataccgt caaccgctgg
    10441 cgcggagtta tccagcgcat gagcgatggc aaaacctaaa cgcgcctgat cttcaaccgg
    10501 tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg tcatcaacgt tggtaacaaa
    10561 cagcggatgc ttcgcacgtt gaccgatgtt gaggattgcc gcaatctgcc agtcagccac
    10621 tttctgtgct gccgccattt cgcgcgcttt accttttacc gcctggcgca ccgccagcgc
    10681 aacgcgcgcg ccggtctggg taacgtcttc gcccagcacc agtaccgcat cgtaagattc
    10741 gatttcgcgc agtgccggcg tataaatgcc gccttcacgc agtactttca gcgccagttg
    10801 cagacgttcc tgctcaccgt gagcgatacc ggtatagaag ttttcttcgc ccaccagctc
    10861 acgcagcgca aagttgcttt ccacgctggc acgcggagaa ccaataccga tgactttctt
    10921 cgactgacgc agaatatctg ctgcgccctg cattgcctgt tcggcgttga gggtaatgaa
    10981 atcatcgcca cgacgctgta ctggctgacg cggacgatct ttcaggttga cgtaaccgta
    11041 accgaaacga ccacggtcgc agaggaagta gtggtttacc gtaccgttgt aacggttttc
    11101 gatacgacgc agttcgccat aacgctcgcc ggggctgatg ttacagccga tggaacattg
    11161 ctggcagatg ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct cggagtgcgt
    11221 tttgtcggtg aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa attcgctttc
    11281 cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg tgcgcaccgt aaacgcccag
    11341 gtccgtaccg tcagcgtaat ctttgtagta acgcacacag cggtagcagg cgatgcagcg
    11401 gttcatttcg tgagagatga acggccctaa atcctgatta cggtgggtac gtttggtgaa
    11461 acggtagcga cggaagctgt gtccggtcat cacggtcata tcctgaagat ggcagttacc
    11521 gccttcttca catactggac agtcgtgcgg gtggttggtc attaaccact cgaccacact
    11581 ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat cggaagccgg
    11641 tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt attgcttcac
    11701 cgcacactgg cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa agtaaggaat
    11761 atcaaggccc agagacagac aagcttccag caggttgtcc gctccgttga cctcgtattc
    11821 tttgccgtct acatgaattg tagccattag catgcttcca gttttctcag tcagagactg
    11881 agcgttaatc gaaattcggt taccagcgct ctttcagcag gttcggctga atcccattaa
    11941 tcaaatgggt attgctgaac ggctgtttga ttcccgcctc aaattcttcg cggaaatatt
    12001 tgatggcgct ctgtaacggc tccactgcac caggtgcgtg ggcacagaaa gttttacccg
    12061 ggcctaagaa tcgacacagt tgctcaagtg tttcgatatc gcccggctgg ccttcgccac
    12121 gctccagcgc acgcagaatt ttcacgctcc acggcagacc gtcgcggcat ggcgtacacc
    12181 agccgcagga ctcacgggcg aaaaactctt ccaggttacg caccagcgac accatgttga
    12241 tctcgtggtc aaccgccatc gccagcgccg tacccagacg gcttcccgct ttaccgatac
    12301 tttcgaattc catcggcagg tcaaggtgcg cttcggtcag gaagtcagtc cccgcgccgc
    12361 ctggctgcca ggctttgaat ttcagaccgt cacgcatacc accggcgtag tcttcgagga
    12421 tctcgcgtgc ggtggtgccg aacggcagtt cccacagacc cggatttttc acccgaccgg
    12481 agaagcccat cagcttggtg ccagcatctt tacttttcga gatgttctga taccactcca
    12541 cgccgttagc gaggatcgcc ggaacgttac acagggtttc gacgttgtta acgcaggttg
    12601 gtttacccca tgcaccggag gttgccggga agggcggttt cgagcgtggg ttagcgcgac
    12661 gcccttccag ggagttgatt aacgctgttt cttcaccgca gatgtagcgc cctgccccgg
    12721 tatggacgaa cagttcgaaa tcaaaacctg ttcccataat gtttttgcca agcagacccg
    12781 cttcggtggc ttcggcaatg gcacggcgta gattcactgc cgcttcgata tattcgccgc
    12841 gcaggaagat atagccacgg taagctttca gcgcaaacgc ggagatgagc atgccttcca
    12901 ccagcaggtg cggcagttgc tccatcagca gacggtcttt ataggtgccc ggctccattt
    12961 catcggcgtt acacagtagg taacggatat tcatggattc gtctttcggc atcaggctcc
    13021 acttcagacc agtcgaaaag cccgcaccgc cgcgcccttt cagaccagag tcttttacct
    13081 gattaacgat ttcgtccgga gacaacccgg tcagcgcctt acgcgcgcct tcgtaaccgt
    13141 ttttgctgcg atattcgtcc agccacactg gctgtttgtc atcgcgcaga cgccaggtca
    13201 gcggatgcgt ttcgggagta cggataatgt ttttcattta taccgctcca gcagttcagg
    13261 gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg atcatcatgt ttggcccttt
    13321 atcacagttc cccaggcagc aagttggcag cagcgtaaag cggccatcaa atgtcgtttg
    13381 ccctggtttg atgttcagtt ttttctccag cgccgcctga ataccctggt aaccgttgat
    13441 atgacagacc acgctgtcgc aataacggat cacatggcgg cctactggct gacggaagat
    13501 ctggctataa aacgttgcca cgccttcgac gtcgctcgcc ggaataccca gcacatcggc
    13561 gatcgcgtgg atcgcgccat ccggcaccca gccacgctgc ttctgaacga ttttcagcgc
    13621 ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa tcgcttcacg
    13681 ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt gcataattag
    13741 cggtccacat ctgacataac aaaatcgata ctgcccagat aaacaatcag gtcagacacc
    13801 aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg cgtacggata
    13861 cgggtgcgat aactcatggt gctgccgtcg ctggtcaggt agtaactgtt gatccccttg
    13921 gtcgcctcaa ccatctggaa ggattcattg gcaggcatca ccggacccca cgacacttgc
    13981 aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg cggcggcgtg
    14041 gtcagcgggt gatccgcttt gaacgggcct tccggcatgt tgttgaggca ctgctcaaga
    14101 atacgcagac tctggcgcaa ctcttctact ttcagcatta cgcgggtgta gcagtcagaa
    14161 acaccaccgc ccaccggaat ttcgaagtcg aagttttcat agccagaata aggacgcgcc
    14221 ttacgcacgt cgaagtcgat cccggtggca cgcaggcccg cgccggtggt gccccactcc
    14281 agcgcctctt tcgcgccgta agcggcaacg ccctgggaac gacctttcag aatggtgttt
    14341 tgcagcgccg ctttctcgta agacgccaga cgtttcggca tccagtcgag gaactcacgc
    14401 agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc cgccaatacg gaaccacgcc
    14461 gggtgcatac ggaaaccagt gattgcttcc accagatcgt aaattttctg acgatcggta
    14521 aaggcgaaga acactggcgt catcgcgccg acgtcctgaa taaaggtgga gatatacagc
    14581 aggtgactgt tgatgcggaa cagttcggag agcattacgc gaatgacgtt aacgcgatcc
    14641 ggcacggtga tcccagccag tttctctacc gccagcacat aaggcatttc gttaacgcag
    14701 ccgccgaggt attcgatacg gtcggtatac ggaatgtagc tgtgccagga ctgacgttcg
    14761 cccattttct cagcgccacg gtggtggtaa ccgatgtctg gtacgcagtc gacaatctct
    14821 tcgccatcaa gctgcaaaac gatacggaaa gcaccgtgcg cagacgggtg gttcggaccg
    14881 aggttgagga acatgaagtc ctcattttcg gtgccgcgct tcatccccca ctcttccggt
    14941 ttgaaggtca gggcttccat ctccagatcc tgtttggctt tggtcagctc aaacggcgag
    15001 aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt gacctttcca ggtttgcggc
    15061 atcatgatgc ggcgcaggtt cgggtgaccg tcgaatataa tgccaaatag atcccaggtt
    15121 tcacgctcat accagttagc gtttgggaat aatttggtaa aggtcggtac gtgcaggtcg
    15181 ttttctgcca gcgccacctt cagcatgatg tcgcggttac gatcgataga aatcagatgg
    15241 tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag acgttcgtcc
    15301 atgccgtgta agtcaaacag catgacgtaa ggtttcggca gtttctttaa gaaatcgccc
    15361 acttccagta attgttcacg cttgatccac acaacgggaa ccccggtgcg agtcgcctga
    15421 acagtaaagg catccggccc aaaacggttg cgcagttcgc caatcaccgg atcatcaaga
    15481 tgatcgcggg tctgccaggc gggttcttgc gcggttaagt cggtcatatt gttcaccatt
    15541 gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc gaaatagatt taggaataag
    15601 cgaagaaaat tccctttgcc gacaggcgca aattaaatct cgtcaggtgt acgcaggttg
    15661 gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg attgcatatt ggcgcgataa
    15721 acgccctgat cgccaaccac ccaggagagc ggacgacgtt ctttaccgat agattcctgc
    15781 aacagcatca gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg gatatacaca
    15841 tcaaccggga tgaatttatc gacgccctgc acaacggaat aaatatcgta cataccgccg
    15901 gagttggcac aggcacccat tgagataacc catttgggtt ccagcatctg gtcatacaga
    15961 cgctgaataa ccggtgccat tttggtaaag caggttcctg caaccaccat caggtcagcc
    16021 tgacgcggcg aagcacgcaa tacttcagcg ccaaaacgcg ccacgtcatg caccgcggta
    16081 aacgaagtca ccatctcaac gtaacagcag gaaagaccga agttatacgg ccaaattgag
    16141 tttttacgac cccagttaac catgtcattg agcttgccca taaacacgtt tttgttaact
    16201 tcttgctcca gagggtcggt tacgatctcc tgcttttgca gggggtaacg gtcgttctca
    16261 ccgttgggat ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg ttagcgttga
    16321 cgattagcga tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg cgcgggcgtc
    16381 cagtccagcg cgccaatacg caccagataa accagacctg ccagtaacac aaaaataaaa
    16441 attgcagctt ccacaaagcc gacccagccg ctttcgcgga tagaggttga ccatgcgaac
    16501 agatacagcg cttcaacgtc gaagataacg aagaacatgg ccaccagata aaacttggcg
    16561 gacaggcgta agcgggcgga gccgaccgag tcgataccgg attcaaacgg aacgtttttt
    16621 gacctcgcgc gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag gcaacacagg
    16681 ccaatggcaa cgataagaaa gatagcgaat gcccagtgat gagcgatgac ttcagtggat
    16741 gttgacatac tcattgctta ctcatcaaaa gtagcgcctg attctctgct ctttacggca
    16801 gatggacgcc acatcgattc atggggagga ataaaaaaaa ccttacaata actgcagaaa
    16861 ctattttata cagctaattg atgtggtctt ttactccttt ctataacctt ttgtcaactt
    16921 taacaaaggt ttcttcacac tgatttacac aatgacaaca acatttgcat ttggcgccct
    16981 ttctccccag atcattgacg actccaggaa aattagatgt tgttgaatcg tgtccgttgt
    17041 gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt cctcccccca
    17101 aaagagagta ttttcttgat ctgtgacacg cttttgtcat tctataacaa aaatgcagca
    17161 acaaattcac gtatttttta acattattgt agcaggagat ttttttcagg tgattatttg
    17221 tgcgtacggg gcgtgaatct ctgatgattg aaaaatgaac agttttgtac gttctgcacg
    17281 atacgaggaa ggctttttac taaaaaaaag ccgctggggt ttaaaataaa cccagcggtt
    17341 cgtttttaca ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt
    17401 caatcaacaa tgagtcatct ccttccggag cagacattgg gctgtattgc cacggattat
    17461 ggtagctctc catcgcctga tagatcacct gggccagctc attattactg gacgggtcat
    17521 agcacagcaa atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac
    17581 gcaggtccgg actcatcatc tcgaccggcc ttgccgtcac gccaagaccg gctttcactg
    17641 ccgcacgaac ggccggaagc gtcgatgcga cataagccag tcgccaggga atatctgctt
    17701 tattaagcgt cgccagcacc atatcgcgaa acggacttgg atcatccagc aacacaagag
    17761 ggatcggctc acctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg
    17821 tacgcaggtt caatgcctta aaggtagaag gacgatgggt ggttaccatc aaatccactt
    17881 cctgggattc cagcatttct gccatgtagg cattacgttt aacgcggaca tccagcgcta
    17941 gtttcggata aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt
    18001 catctgaagc accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg
    18061 cctcatcatt aaaacgcagg attttcctgg cgtaaccaag aagttgaatg ccatgttcag
    18121 ttaacagttt gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac
    18181 gctgcatttg ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa
    18241 aagtgttcag atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta
    18301 tcggacgatt tgcacttatc atatattatc acttactggc ggctcatact gagctggtta
    18361 atgctgtgca cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc
    18421 cggaacccgg acgaaagtaa aaatgcatat gagttgcact aaaaaagtga ctcacattgt
    18481 tccgttataa tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa
    18541 gttactcact cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc
    18601 ttgttccact ctcgtacagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg
    18661 aggcggcatc aagagcaggg atccgtcatt tatgcaagca ttttacccca aaaacctttt
    18721 atttataagg gtcattgcga attatctgat gcaaag
//
LOCUS       DADTFG010000094        18724 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.094, whole genome shotgun sequence.
ACCESSION   DADTFG010000094 DADTFG010000000
VERSION     DADTFG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.094"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(549..1301)
                     /locus_tag="J1138_003036"
     CDS             complement(549..1301)
                     /locus_tag="J1138_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBA8454804.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMAMDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEYCAGTGFHNVLREVVKHSRSGESVIQEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHSTPVYKVCGRCNGNRFSRLPTTLARCHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTR"
     gene            complement(1315..2364)
                     /locus_tag="J1138_003037"
     CDS             complement(1315..2364)
                     /locus_tag="J1138_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBA8454805.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRNIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLPAEKNCSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPSQEKTVVSITVDPESPESFMKLPKRHRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHDELHANTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            complement(2366..2644)
                     /locus_tag="J1138_003038"
     CDS             complement(2366..2644)
                     /locus_tag="J1138_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454806.1"
                     /translation="MARNVKYYNSDNSPVLACTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(2711..2962)
                     /locus_tag="J1138_003039"
     CDS             complement(2711..2962)
                     /locus_tag="J1138_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454807.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            complement(3179..3391)
                     /locus_tag="J1138_003040"
     CDS             complement(3179..3391)
                     /locus_tag="J1138_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA8454808.1"
                     /translation="MLDTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            3436..3599
                     /locus_tag="J1138_003041"
                     /pseudo
     CDS             3436..3599
                     /locus_tag="J1138_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3939..4604)
                     /locus_tag="J1138_003042"
     CDS             complement(3939..4604)
                     /locus_tag="J1138_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBA8454809.1"
                     /translation="MTVADFKRPKLELPNGAKKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAKQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAYYPGMVYWD
                     YNWRKQGGSSRMIEISKREQFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     GQE"
     gene            complement(4658..4891)
                     /locus_tag="J1138_003043"
     CDS             complement(4658..4891)
                     /locus_tag="J1138_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBA8454810.1"
                     /translation="MSTSATILDMCCGSRMLWFDKNDEWSIFSDIRKEEHSLCDGRHP
                     IINPDIIADFRALPWLSALHQKQTLPDLDICFR"
     gene            complement(4888..5310)
                     /locus_tag="J1138_003044"
     CDS             complement(4888..5310)
                     /locus_tag="J1138_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBA8454811.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRVLTFYQGNYREVML"
     gene            complement(5351..6316)
                     /locus_tag="J1138_003045"
     CDS             complement(5351..6316)
                     /locus_tag="J1138_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454812.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKKLGLLRCEKLNKSKRDMTNFYTINY
                     ESELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRSNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(6297..6818)
                     /locus_tag="J1138_003046"
     CDS             complement(6297..6818)
                     /locus_tag="J1138_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454813.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGTVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(6802..7029)
                     /locus_tag="J1138_003047"
     CDS             complement(6802..7029)
                     /locus_tag="J1138_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8454814.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            7107..7514
                     /locus_tag="J1138_003048"
     CDS             7107..7514
                     /locus_tag="J1138_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8454815.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            7707..7838
                     /locus_tag="J1138_003049"
     CDS             7707..7838
                     /locus_tag="J1138_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HBA8454816.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARR"
     gene            7864..8439
                     /locus_tag="J1138_003050"
     CDS             7864..8439
                     /locus_tag="J1138_003050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454817.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDA
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ARRTIISTSPNSRTNKSHFERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            8926..9114
                     /gene="dicB"
                     /locus_tag="J1138_003051"
                     /gene_synonym="ftsT"
     CDS             8926..9114
                     /gene="dicB"
                     /locus_tag="J1138_003051"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBA8454818.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            9111..9302
                     /locus_tag="J1138_003052"
     CDS             9111..9302
                     /locus_tag="J1138_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HBA8454819.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            9396..11867
                     /locus_tag="J1138_003053"
     CDS             9396..11867
                     /locus_tag="J1138_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBA8454820.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQTANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            11955..12191
                     /locus_tag="J1138_003054"
     CDS             11955..12191
                     /locus_tag="J1138_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBA8454821.1"
                     /translation="MIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREYKHVAH
                     DGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            12226..13506
                     /locus_tag="J1138_003055"
     CDS             12226..13506
                     /locus_tag="J1138_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA8454822.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRLAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(13526..13636)
                     /locus_tag="J1138_003056"
     CDS             complement(13526..13636)
                     /locus_tag="J1138_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA8454823.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAC"
     gene            complement(13694..14713)
                     /locus_tag="J1138_003057"
     CDS             complement(13694..14713)
                     /locus_tag="J1138_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HBA8454824.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFELDQKHCCKVL
                     LTFSE"
     gene            complement(14725..15939)
                     /gene="rspA"
                     /locus_tag="J1138_003058"
     CDS             complement(14725..15939)
                     /gene="rspA"
                     /locus_tag="J1138_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBA8454825.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(16145..16471)
                     /locus_tag="J1138_003059"
     CDS             complement(16145..16471)
                     /locus_tag="J1138_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBA8454826.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            16606..16947
                     /locus_tag="J1138_003060"
     CDS             16606..16947
                     /locus_tag="J1138_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBA8454827.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            16982..17542
                     /gene="speG"
                     /locus_tag="J1138_003061"
     CDS             16982..17542
                     /gene="speG"
                     /locus_tag="J1138_003061"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HBA8454828.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(17545..18255)
                     /locus_tag="J1138_003062"
     CDS             complement(17545..18255)
                     /locus_tag="J1138_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBA8454829.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            18363..18668
                     /locus_tag="J1138_003063"
     CDS             18363..18668
                     /locus_tag="J1138_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBA8454830.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDNAPAEPQ"
BASE COUNT         5197 a         4615 c         4136 g         4776 t
ORIGIN      
        1 gcaatttata attttcatcc tactgttttc cctgcactgc caacacaagt ttcatcgcaa
       61 cactctgaca agacaccaat aaaatcagtt tcgttatcac cttaattttc ttcatgggta
      121 acaaaccggt aatacatcga tagccagatt tccggcgtaa gccatgtatt tgcagcctga
      181 ttgatgcgcg ttactgttac ctcttccctg catcaggatg gcttctccag atgatatggt
      241 taatcacact ggaacattca cgcataaccg tttccttcat atcctgccgt tttagcatgt
      301 cttttcccct tcttgttaat atcaataact aacacaaatc taaaaaagcc agcaatgtaa
      361 aaaaactgca ttcacggcaa ccataatggt taccgttctg cacaaagttt atgtccacaa
      421 aaaaaccact ctgcgcggct tattgatatt ttccaccggt atgcatataa ggctcatcat
      481 tggaaaactc tttgcacgtt tccttagaaa atacaatcgt tatgtcgcca tcgttcggtg
      541 aaaatcattt atctcgtcac ttttcttaat tgcacctcag catatgcttc ttcctgccag
      601 cactttgtaa ccagtttatc aatgacatct gcatatcctt tgtaccactg ataatccgtc
      661 aggtctggta ccagcttctg gacatgacac cgcgccagtg tcgttggtaa acgactaaac
      721 cggtttccat tgcaacgccc acaaacctta taaacaggcg tgctatgaag ccgggttctt
      781 ttttcatcca ggacaatacc tttcccctta caccctctgc acgctgtgct gacttctccc
      841 ttaccatggc aatgctgaca tagttccttc acccactctt cctggataac agattcccca
      901 cttctggagt gtttcaccac ttcgcgcaat acattatgaa atccagtacc agcacaatac
      961 tcacagcgag ccttacttgc cgcagacctg gaataatcag caaaggcaaa attcacaagg
     1021 taagggatga tctgtaaccg gatttcttca ctcaatttgt tcaatgtcgg attatccatt
     1081 gccatcgcgt aattgagcag accttcaatc gcaaactgag gatcctgaac accaactttt
     1141 gccaggaata aggcaaaacc aagcggagct ttcgactgca ccatcccctg cgcagccatc
     1201 acatctgtta ttgttaaacc acccgagcct gtcgccggtg cgtcatcact caattttgga
     1261 gattttgggg agtaatattt tggtaaagct tcaaggttca tgctcgttct ccacttacgc
     1321 cagtacgcct attgccagcg cacgatcgat aaaacgaaat atcagctcca gctgggagcc
     1381 atacttctct tcaaatgcca cggtattcgc atgcagctcg tcgtgatgct ttctgcacaa
     1441 aggcaacaca aagagatcat gtgctttggt tcccattccg ccctgcccgt gaccaatcag
     1501 atgatgcgga tcgtcggctg gcataccgca gcaagcacac ggctgtgtct taacccagcg
     1561 agtgtatttc tccttaaccc aacggtgacg tttaggcagc ttcatgaaag attccggaga
     1621 ctctggatca acggtgatgc ttaccaccgt cttttcctgt gatgggtttt gttgctggtg
     1681 ggcgtgaggc aacggtgcaa gattttttgt gcgctgtttc aatatgctgg tggcggtctg
     1741 ctctcccggt acgatgtcgc tttcgcggta caccgagcag tttttttccg ctggtaatcc
     1801 cagcgagcga cgtaataccg cttccggtag cgcgtccgcc acctgattgc ggaccgccca
     1861 ccaggataat tcagccagag ataattcacg ctcctgcgta ccgcttattg cgtgaccgat
     1921 gacgtcaatc atccatgctg acaggttttg atgagcaagt tgctcgagtg attcggatgt
     1981 ctggtcacgc agctggttgt cgcagtgcca gcacaacacc attgcgccgg taccataacg
     2041 gtgaataacg gtttcgctgt gatgataatc gccgtgtggc cactggcagg atttaatatt
     2101 gcgcaacagc cagtcagaca atgcaccagc accaccagca gcacgaatta cccgtgcgtt
     2161 actgaaaaac ggcagcaatg ttttgtcttc caccagcggc tggcgaacgg caggaacgac
     2221 cccggacggc agattacgca tgcttttcgg ttccggctcc accagtaccc gggtattgtg
     2281 gaataccggc atggattcac ggcccggctt aacgaccacc agcccgagtt ccggtaccag
     2341 aacaggtcga agtaataccc gcacgttacc tccagatgcg ttgctggaat gtacgggacg
     2401 gacgcggtgg gcgttcggag taaggcaatc tgactgagat tatccagtga cggtagtcga
     2461 gactaagggc tttcttaacc tcgtatccgc gcctgcggta acactgaatt atccattcag
     2521 cctgctcttc agtgcatgga gggtgctgga accattcaga cttgaatgcg tgagaatacc
     2581 gctcgtgcgt gcaggcaaga acgggcgaat tatcagaatt gtaatatttt acgttgcgtg
     2641 ccatcggttt tctccggtgg cacggtgtta ctcagcggga gttcagcccc gcgcaagatt
     2701 gtagatgagt ttattcttct gcaaaagctg aaaagcctgc ttttattccg atctctttca
     2761 gtgcctgtaa tgaagtgaca aactcacctt cgcgcaagat aaatccgtct gtcactcgac
     2821 catccacaaa attaattaac gcagccccat tctttcgcaa acacataatg cggtaatgac
     2881 taacaagatt tccattttca acgcacacag catagaggcc atcttcacaa aaaattttac
     2941 gcagttcttc gatgttcatc atcagaatcc ttccggataa ttagctctcc cctttaaggg
     3001 accatccctc ttatccctgc gcgctactta agtatttttg attctattcc ggcaccgtcc
     3061 agaacttcaa acgcgttgaa aataaaaaca aaaacccgcc gaagcgggtt aagtgcgggt
     3121 gcgttgagga tgcctgccac atcagaggtg gcgagggatt tctccctcgc cgggtctctt
     3181 actcctcagg ttcgtaagct gtgaagacag cgacctccgt ctggccggtt cggattcgta
     3241 cctcgcagag gtctttcctc gttaccagtg ccgtcactat gacggttaaa caaatgacga
     3301 tcagggcgat taacatcgcc ttttgctgct tcatagcctg cttctccttg cctttcggca
     3361 cgtaagaggc taacctacat gtgtctagca tgaaattggc ctcagattaa tgttaagcgt
     3421 cttgcaggac gcgtaatgtt aactggggct tttctctatc tgccgttggt gttcatgccc
     3481 gaggcagata gcctcaagca cccgcagcca ttctacttaa ctaccgttac ctcgccaata
     3541 tgaaatcaat cagtaaggtg atccataaga acaataaatt gccattacag ccacaatagc
     3601 cagcgtgcat ttgagaacca gcacataaat ctcctgctct tgacgtatac aggcactgat
     3661 aaatatgtgt cttcgtgctc gttacttcaa tacgtaagcg tcagttgcgc gtaatcaaat
     3721 cagacaaccg actttaaacc agggacaaac agcaggtaaa ataatcaaac gttattttaa
     3781 gccatatcat ggcttcaaga tttcctctaa tttcaacaag atgagcaggt cattgcttgc
     3841 actgatgacc tgctcattaa tcatgattat ttgaaaatta aggtaggcgt actatatata
     3901 gaaacactgc tacgctccgt tgttggcata atataaaatc attcctgccc ttctttaccg
     3961 tagtggagtt gaccaatttt gattagaggg cgtccctgag atttgcggtg tagattggta
     4021 tcgcgcagtg aatacacgca gccacaatat tcctgctgat agaattgttc gcgcttgctg
     4081 atttcaatca tgcgggaaga gccgccctgc tttcgccagt tataatccca gtacaccatg
     4141 cccggataat acgcgacggc tcgccgtcca cagtcgttaa cctgctgcat atttttccag
     4201 cgtgaaatgc ccagtgagct actgatcaca ctgaaaccat tttcagcggc atacagcgca
     4261 gtccgctcaa aacgcatgtc aaaacacatg gtacaacgga tccctcgttc aggctcccat
     4321 tccattcctt tggctcgctc aaaccagttg tctgtgtcgt aatcagcatc aataaatggc
     4381 acgccgtgtt gtttagcaaa gcgaatattc tcatccttac gaattaaata ctctttctga
     4441 ggatgaatgt tcgggttgta gaaaaagatg gtgtagtcga ttcccgaggc ctgaagcgcc
     4501 tccatcactt caccggaaca tggagcacag caagagtgca gtaatagttt ttttgccccg
     4561 tttgggagct ccaatttggg gcgtttgaaa tcagcaacag tcataaatgt gtttatgagg
     4621 ttcatgaaaa tagcaaaaag tgtagcatca gaatgggcta tcggaaacag atgtctaaat
     4681 ctggtaatgt ctgcttttga tgcaaagcag acaaccacgg cagcgcacga aaatcagcga
     4741 taatgtcggg attaataatc gggtgacgtc catcacatag cgaatgttcc tctttcctga
     4801 tatcgctaaa tatcgaccat tcgtcattct tatcgaacca gagcatgcga ctgccacagc
     4861 acatgtcgag aatggtagcc gatgtactca cagcatcacc tcccgataat tcccctgata
     4921 aaacgtcaga acacgctgca taacttcgct cttccggcac tcgcgacaga ttatgtttag
     4981 acgactgtcg tagcggcgta tttctccatc aggtaatgac cagataaggt ccggatcaac
     5041 cacagcaggt ttcttcacct ttgccctcga gagttttttg cgggcgtttt gccagtcctt
     5101 acgcgcctgt tcagacggaa ataacccgta gccggagttg tatacatcgc cactggctac
     5161 cagctctctg gcaagaacgc tcatcagata tctagtcgca cctgttttag cttccagttg
     5221 ccgtaacgtc tcacgaccgc tctggcgcac gagttccacc acctgccctt taattttttc
     5281 ccgctcttct tgtgtaaaaa cttttgccac aagtccccct taaaattacc tcatgacctg
     5341 aaatcaacat ttatcctctg aaaccaggcg gaatttctgt atccggttca gaaatatgat
     5401 taacacaacg ctgtacaggt gaacgtccca ggcggatgac cagttcgtcc catttttcgc
     5461 ggagctttga cgggctcatg atgtttttta cccagaatgg atctcgctga acccgaccaa
     5521 acatttcgca aatttgtctg tggcttctgc catccagcat ccgcattgtg cgcacgtcat
     5581 tggcccaggc agtccagtta ggctcttttg gtcgcatgat ctcgccatca tcactggctg
     5641 cctgttcgta gagacccacg atccgccccc agatccactg cgcacacgcc aaatcctccc
     5701 tgctacccca ctggcgtttt ttcgcactaa acacaaccgc gtcggagttc cgggttaaaa
     5761 aatcctgttc agtcgtctgc gggtccggtt gcgaagcttc cggacgaaaa gtgtttttat
     5821 tctctgtagt aatctctgtt gtattctctg taagatcatc aggccatttt gacccgatga
     5881 cattggatcg ttttgaacca atggagcgtt tcattttgac ctcttccatc gtgtcatttt
     5941 gacctgatgg agcggcgcat tttgaaccga tggattcgct caatttgcca tcatctaaaa
     6001 gctcgctctc gtagttgatc gtgtagaaat tggtcatatc gcgctttgat ttattgagct
     6061 tttcacaacg caaaagcccc agctttttca gacttgcaaa cgcgcgcttt aacgttgact
     6121 ctgaccagaa tgggaactgt tccagccatt gttccgttgt gttgtaaatc cagcgaacac
     6181 catcacattc catgccggaa ttggtatctc tcaaccagta atgcaactgc tgcaacacaa
     6241 tggcttcatt taagccaatc ttcatcgcaa gctgtgtgtt tataaccagt gggcgttcag
     6301 caaaaagaag gctcataatt ccatccagct ttttgttggt attgctgtcg atacgcaagt
     6361 ttgaaagcaa ttgctttttc tataagttcg tcagtttcac gatccactac ggcaggatca
     6421 gcaaaaagca gtccggactc caccacatcg ccatattctt tgtttaaccc ggcgatcata
     6481 tacgtgatgc tttttccgtc actaatttca cgatacaacc tgaaatcatt aatccggata
     6541 gcctccataa ttgcaggcac tagcgccgtg aacttttcac gcttatccct ggtgtcgata
     6601 gccttccagc gttcgaatat cttcactcga ttaacgccaa gcgctcgctg atcaaccgtg
     6661 ccaccttcat ctgtgacacg ctgaacatcg atgttcgggc gctctttcaa agcccagaat
     6721 gcttcagtga ttaatatcgt cgcctgctcc tgtgtcattc ctggtcgaca tatccaggca
     6781 tccagagcct cacgagcctg ttcaggagtg attttcattg ttcaaccgcc ccgcccgctt
     6841 cgtcttacga tattcgtcat aaaccttggg atcatactga agctcgccgc cagatgcctc
     6901 ctgtagacgc atcgcgcgac cttcaggaac cagctcttgc cattgagaaa cagcagatgg
     6961 gtcaacgcca gcagctttcg ctactttggc tttcgtaccg taaaaattaa ttacgtctga
     7021 tttaaacatc gcacctccaa tattgagttt tctcaatgct aatcactcaa ggaatctcaa
     7081 gtcaagggtt attaagatat ctaaatatga acgagaaaac tttaggtcaa cgcattagag
     7141 aaagacgtaa gcaggtaggc ttaagtcaaa acggtttaag caaagctgct ggcgtatctg
     7201 gctcatcaat ttcattatgg gaaagtgacc atacagcccc gcgcgggcaa aatttgcatc
     7261 gcctcgccga ggtattgcaa tgttcaccaa cctggatact gtttggcgac gaggacaaaa
     7321 caccagctcc ccctgtttca ctcgatagtg ccttagactt atcggaagat gagttagaga
     7381 tgctgcgttt gtatcgtgca cttcctaaat cagagcaaca agcacaactc agcgaactcc
     7441 gcgcccgcgt tgagaatttt aatcgcctgt tcaccgagct attagaggct cgcaaacgca
     7501 acaagcatca ataatcatcc cttcacaaaa ttttaaagcc ttacatttca atgtattggc
     7561 tttatctcgc gccaatactt gagttttctc atcaaaaaac cttgacgaaa aataatgaga
     7621 aaactaaatt acctccatca agacaccgca cggtgttctc agcaaacagt tccgctaccc
     7681 cggcgttaag gggaaatgag gtcagcatgg atactatcga tcttggcaac aacgaatctc
     7741 tggtgtacgg cgtgtttcca aaccaggacg gcacgttcac cgcaatgacg tataccaaaa
     7801 gcaaaacgtt taaaaccgaa aatggtgccc gtcgctagct ggaaagaaac tcaggtgagt
     7861 gatatggatt tcgacacaat catggaaaag gcttacgaag aatacttcga aggccttgcc
     7921 gaaggcgaag aagctctcag cttcagtgag tttaaacagg cactcagaat aagaatgtgc
     7981 tctcacaatg acgcggagca caaatatgag aagcaaaatc agaccgcaga aaattttgtt
     8041 ctggaacccg gagaaacgct tttcaaaatt cccgttacgt gccccatttg cggttttaca
     8101 tcagaagaac ttgacgactc ctgtaacaat caggaaacaa ccaagtatgt cgaagatgat
     8161 accgagtgcg cacgaagaac tattatatcc acgagtccaa actccaggac caataaatct
     8221 cactttgaga gggtgattaa tccactcccc caaaccaata aaaaagatgc cggaggacaa
     8281 aaaacccaat ggaacaacgg gatatctgtc aaaaaaagac gttccattaa agacaaacaa
     8341 cgcagcgcca acaactgtaa gcgctttata ccagtaatca attttcatgt tcttaagcgg
     8401 atttattggt ggttgcgaca ttgcttaatg aatccttaaa actgtggtga ttttaaggat
     8461 accacctcgc ctgacgtggt taaaagcagg cacacaacac gaaagcgcac ggcgaagttc
     8521 gtctcactgt acggtgtcgt taaatttaat tcgaccgtgc gcttccggtt gtggcaaccc
     8581 gcaaaatggc gcggcggtaa gtatggcggg gttattcctt ccccgttgag gacaccgagt
     8641 tgtcaggttg accatacgct taagtgacaa ccccgctgca acgccctctg ttatcaattt
     8701 tctggtgacg tttggcggta tcagttttac tccgtgactg ctctgccgcc gtttttaaag
     8761 tgaattttgt gatgcggtga atgcggctaa gcgcacgcgg aacagttaaa accaaaaaca
     8821 gtgttatggg tggattctct gtatccgacg ttaattgtta actggttaac gtcacctgga
     8881 ggcaccaggc actgcatcac aaaattcatt gttgaggacg cgataatgaa aacgttatta
     8941 ccaaacgtta atacgtctga aggttgtttt gaaattggtg tcactatcag taaccctgta
     9001 tttactgaag atgccattaa caagagaaaa caagaacggg agctattaaa taaaatatgc
     9061 attgtttcaa tgctggctcg tttacgtctg atgccaaaag gatgtgcaca atgaattcag
     9121 catttgcgct tgttctgaca gtttttcttg tttccggagt gccagttgat attgcagtca
     9181 gtgttcacag gacaatgcag gagtgtatga ctgcagcaac cgaacagaaa attcccggta
     9241 actgttaccc ggtcgataaa gttattcacc aggataatat cgaaatcccg gcaggtcttt
     9301 aaaacagttc cgtaataaat atccggtttc attcttatat gccagcaatg gcagggattt
     9361 gttcatcctt aaatctgtca tgaggttaaa acaaaatgag taaagtcttt atttgcgccg
     9421 ctattcctga cgaactggca acaagggaag aaggcgctgt ggctgtagcc acagccattg
     9481 aagctggcga cgaacgccgt gctcgagcaa aatttcactg gcaattcctg gaacattatc
     9541 cggctgctca ggactgcgct tataaattta ttgtctgcga ggataaacct ggcatacccc
     9601 gccctgccct cgattcatgg gatgctgaat atatgcagga aaaccgctgg gatgaggagt
     9661 ctgcttcttt tgtcccggtt gagactgaat ccgatccgat gaacgtcact tttgacaagc
     9721 tggcccctga agtacagaac gctgtcatgg ttaagttcga cacatgtgaa aacatcaccg
     9781 ttgatatggt tattagcgca caggaattgt tgcaggaaga catggcaaca ttcgacggac
     9841 atatcgttga agcgttgatg aaaatgccag aagttaacgc catgtatccg gagcttaagt
     9901 tacacgccat tgggtgggtt aagcataaat gtattcctgg tgctaaatgg cccgaaattc
     9961 aggcagagat gcgcatttgg aaaaaacgtc gcgaaggtga acgcaaggaa accggaaaat
    10021 acacgtctgt tgttgatctc gcccgcgcca gagccaatca acagtacact gaaaattcaa
    10081 caggaaaaat cagcccggtc attgctgcca ttcatcgcga atacaagcag acatggaaaa
    10141 cactggatga cgaactggcc tacgctctct ggcctggtga tgtggatgcc ggaaacattg
    10201 acggcagcat ccatcgctgg gcaaaaaaag aagttatcga caacgaccgc gaagactgga
    10261 agcgtatctc ggcatcaatg cgcaaacagc ctgatgccct tcgctacgac cgccaaacta
    10321 tttttggcct tgtccgtgag cgtccgatcg acattcacaa agatcccgta gcactgaaca
    10381 aatatatctg cgaatacctg acgacaaagg gcgtgtttga gaatgaagaa acagacctgg
    10441 gcactgttga tgttcttcag tcatcagaaa cacaaactga tgcagtggaa actgaggtat
    10501 ctgatatccc aaaaaatgaa accgcgccgg aagctgaacc atctgtagag cgtgaggggc
    10561 cgttctattt cctcttcgca gataaggacg gagaaaaata cggtcgcgca aacaaacttt
    10621 ctggtctgga taaggcactg gctgctggcg ccactgaaat cacaaaagaa gaatattttg
    10681 cccgaaaaaa tggcacatac acgggcttac cgcaaaatgt agataccgct gaagattcag
    10741 aacaaccaga gccgataaaa gttaccgctg acgaagtaaa caaaattatg cagacagcca
    10801 atatcagcca gcctgacgcc gataagttgc ttgctgcatc acgtggtgaa tttgttgaag
    10861 ggattagtga cccgaatgat ccgaaatggg ttaaggggat ccagacccgc gattctgtga
    10921 accagaacca gcatgaatcg gaacggaact accaaaaagc ggaacaaaac agtccaaatg
    10981 cgttacaaaa cgagccagaa acgaaacagc ctgaaccagt ggcgcaacag gaagtggaaa
    11041 aagcctgcac cgcctgcggt cagaccggcg gcggcaactg ccctgattgt ggcgcggtga
    11101 tgggcgacgc aacataccaa gaaacattcg atgaagagta tcaggttgaa gttcaggaag
    11161 atgatccgga ggaaatggaa ggcgctgaac atccacacaa agagaacact ggcggcaatc
    11221 agcatcacaa tagcgataat gaaactggcg agacggcaga tcacccaatt aaggtgaacg
    11281 gtcatcacga aatcacatcc accagcagga cgtgtgacca tctaatgatc gaccttgaaa
    11341 ccatgggaaa aaatcctgat gccccgatca tctcaatagg tgcaatattt ttcgatccgc
    11401 aaaccggaga tatgggaccg gaatttagta agactatcga tctggaaact gctggcggag
    11461 tcattgatcg ggacaccatt aaatggtggc ttaagcaatc acgcgaagcg caatctgcca
    11521 ttatgaccga tgaaatcccg ttagatgatg cactgttaca attgcgggaa tttatcgacg
    11581 aaaactccgg tgaatttttt gttcaggttt ggggaaatgg agccaacttc gacaacacga
    11641 ttttgcgccg ttcatacgaa cggcagggga tcccctgccc gtggcgttac tacaacgatc
    11701 gcgatgtacg cacaatcgtt gagctgggga aagccataga cttcgatgcc agaacggcta
    11761 ttccattcga aggtgagcgc cataatgcac ttgatgacgc ccgttaccag gcaaaatacg
    11821 tttcagttat ctggcaaaaa ctgatcccga gtcaggctga ttcttaatgt tcaactgtcg
    11881 ccggttgtga ctggtattct gcaaccggcg ctcgtctgat gtaagagata aagaaatcga
    11941 tgagcgaagt aatcatgatt gtctctcccg gcaaatgggt atccgaagag cagttaattg
    12001 cgctgaaagg aataaaaaaa ggtacgttaa aaaaggcccg ggaaaaatcg tttatggaag
    12061 gaagggaata taagcatgtc gctcatgacg gtatgccatg ggataacagt ccatgctttt
    12121 acaacctgga agaaattgat cgctggattg agcgccaggc atcagcgaga ccaagacgtc
    12181 atcttacttg actaaaagcc acactaacta atgagagaag ttgaaatgaa atatccgaca
    12241 ggcgtggaaa accatggagg gaaattacgt atctggtttg tttataaagg cgtaagagtc
    12301 agggaaaatc tgggggttcc tgacacagca aaaaacaggc gccttgcagg tgaactacgc
    12361 tcctctgttt gttacgcaat aaaaactggt gttttcgact atgcaaaaca gtttccctcc
    12421 tcacgcaatc tggaaaaatt tggtgaggcc cgacaagatt taaccataaa agaactggct
    12481 gaaaaatttc tggcactgaa agaaactgaa gtcgccaaaa catcactcaa cacataccgt
    12541 gccgtcatca aaaatatcct gagcataatc ggtgaaaaaa atcttgcctc atcgattaat
    12601 aaagaaaaat tactggaggt tcgtaaagag ctactgactg gataccagat ccccaaaagt
    12661 aactatattg ttacacaacc agggagatcg gctgtaactg taaataatta catgacaaat
    12721 cttaacgccg tgttccagtt tggtgttgat aacggttacc tggcagataa tccgtttaag
    12781 gggatctcgc cattaaagga atcaagaacc attccggatc ctctttcgcg ggaggaattt
    12841 atccgtctta tcgatgcgtg cagaaatcag caagcaaaaa atttatggtg tgtttctgtt
    12901 tatactggcg ttcgccctgg tgagctgtgt gcacttggat gggaggacat agatctgaaa
    12961 aatggaacaa tgatgatcag gagaaattta gcaaaagacc gtttcacggt accaaaaaca
    13021 caggcgggaa ccaatcgggt cattcatctt attaagccag caatcgacgc tctccggagt
    13081 cagatgacat taacgagact gagcaaagag catatcattg atgttcacct cagagagtat
    13141 ggcagaacag aaaaacaaaa atgcaccttt gtttttcaac ctgaagtgtc agcgagagta
    13201 aaaaattatg gtgaccattt taccgttgac tcaataaggc agatgtggga cgcagcgata
    13261 aaacgtgccg gactccgcca tcgaaaatca tatcagtcga gacatactta tgcctgctgg
    13321 tcgctgacag ctggtgctaa cccggcattt atagcaaacc agatgggcca tgcagatgcg
    13381 caaatggtat ttcaggtata cggaaaatgg atgtctgaaa acaataatgc acaggtagct
    13441 ttgttaaata cacagttaag cgagtttgcc ccaaccatgc cccataacga agcaatgaaa
    13501 aattaattta atatttatca aatagttaac acgcatgact cttgaaatcc ataaattcaa
    13561 gcgcagtgcc caaccatccc gatactgctg ctttcaccaa atccttagta cttctttcgt
    13621 gtttttctat tgtcataatg gttatctcta aaaaagaggt aagatgcgta ctacttactc
    13681 gccgttattg gtattattca gaaaaagtga gtaagacttt gcagcaatgt ttttgatcca
    13741 gttcaaataa actaatggca tccgcaacat gctggaaatc aaatgtatgg gtaattagtt
    13801 tttctggttt aattaaccct ttacttaacc agtcgataac gaccgggaat ttatttgcat
    13861 ttaagcgtga agagaaaata gagagttctt ttccggtaat tccttgctga atcacttcag
    13921 acggttcact ggagaagccc atcaatacaa tacgtgccgc tggagaagcc agcgttacgg
    13981 cctctttcaa gattgaagga tgacaagcgg catcgataat taaagtcggc ttgatgcctt
    14041 tttcagcgaa actctcgcca agcggtgtct ggctgttatt aatcgcccag tctgccccgc
    14101 tctctttcgc tttttccagt cgttcatcaa tgcgatcggc aacaatcaca tttttaacgt
    14161 tatagacgcc ttttaatacc tgaacgatcg tcaggccgat tgggccggca ccgtaaacca
    14221 gaacggtatc attttcagtc ggttgaccat gaccggtaac gttagccgca atggtaaaag
    14281 gttcgatcat caccgcatat tgatcggcca cagcttcagg aattttccac gcattttttg
    14341 ccggaaccac agcatattca ctgaaaccac cgtcagcgtg cacacctaat acagcaagtg
    14401 tcgtacaaac gttcggctta cctatagagc acggatagca atgcccacag ctgaccaccg
    14461 gatcgacagc aacgcgttca ccgactctgg cgctttccac gccttcaccc actgcatcaa
    14521 tgacgccaaa gaattcatga ccaatgacgc gcggatattt cgcaaaagga ttatgcccac
    14581 ggtaaatatg gctatctgaa ccacaaattc cggcaagttt cacttttact cgtacttcac
    14641 ccgctgacgg ggtgggtatt tcacgttcga taatcgacag ttgattcggt ttttcaatta
    14701 aaatgctttt cattacctta ctccttacca gttccacagc gtgccatctt ccagacgtgc
    14761 gactggcaga taagcaggtt catagggata tttcgccgcc agcttttcat cgaattcgat
    14821 gccaagaccc ggtttttctc ccggatgcat atagccgtta tcgaaagtcc agttgtgcgg
    14881 gaagacttcg agcatttgtt cggaataacc catgtattcc tggacaccga aattggggac
    14941 ccacagatca aagtgcagcg ccgcagccat gcagactggt gacaaatcgg aaggaccgtg
    15001 tgagccagta cgtacctgat acagcgaagc aaaatcggca atccggcgca taccggtaat
    15061 tccgcctgca tgggtcagcg tggtgcggat ataatcgatg agttgctctt caatcagttg
    15121 tttgcagtcc cagatgctgt tgaagacttc acccaccgcg atgggtgtga cggtatgttg
    15181 gcgaatgaga cggaagcatt cctggttttc cgcaggcgtc gggtcttcca tccagaacat
    15241 gcgataatct tcaatgctct taccaaagcg cgccgcttca ataggcgtta agcgatggtg
    15301 catgtcatgc agcagatgtt catcaaaacc aaacttgtta cgtaccgcgt caaacaattt
    15361 cggcatgaaa tcgaggtatt tctccgtcga ccacagctgc tcttccggcc actgtccttt
    15421 ggttgcgggt tcataagcca gacctttacc tttcgacatg ccgtaggtgg ttttcatacc
    15481 agggattccg cactgcacgc ggatggcttt gaatcccagc tcctgatgac gggcataatc
    15541 atccagagct tcatcaatac tgtgaccggt ggtatggcaa taaaccatca ccccttcacg
    15601 agacgcgccg ccgagtaact ggtaaagcgg catgttggca gctttggctt taatatccca
    15661 cagcgccata tcaaccgctg aaatggccga catcgtaacc ggaccgcgac gccagtaagc
    15721 acctttatag aaaaactgcc agatatcttc gatacggtgt gcatcgcgac caataagctg
    15781 cggacaaagg tgatcctgca aataagaggc cacggaaagc tcacgtccat ttagggtggc
    15841 atccccaagg cccgtaatac cgtcctcagt ggtgattttt aatgtgacga aattacgccc
    15901 cggacaggta acaaaaactt cagcctttac gatcttcatg ttcgattcct tgcatcgctt
    15961 gtcgtgatgc atgaaatcta cgcaactgag ctactaccat acaagtatga agatcaaaaa
    16021 aagccggagt gatcacaaaa aaaggcgtat atttgcgccg tgaatggttg acaaaagatg
    16081 aaacagaata ccttttgtca gctgacactt cctcgtatct tattgataaa atggatttat
    16141 gttcctacgt gcgcccccag cccgcaacaa tgatcaacat gccgcaaagc gcaattaacg
    16201 cccccgtcca gtcataaaga gtcagtttca cgccatccac aacgcgcagc caaatcaacg
    16261 ccgtgcagac ataaacgcca ccataagccg cgtaaacacg cccactcgcc gcaggatgca
    16321 acgttaacaa ccagacaaac agcgccagtg aaatccccgc cggaagcaac agccagatac
    16381 tggcgtttcg cttcaaccac agccagggca aaaagcatcc aataatttca cacagcgcag
    16441 tagcaaaaaa tagtaacgtt gttttaatca tctttgtcac ttattgacat cacgtatagt
    16501 tatagggcga cataatatca tcaatataaa caccctcctt gaacgttttg cgttcgcagt
    16561 ggatggtgta gaatcagctt taatcattca tacagggaat gaattatgaa aatcactctc
    16621 agcaaacgaa tcggcctgct cgcttttctg ctgccttgcg cactggcatt gagcacaact
    16681 gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca aagccgccag
    16741 cgcgccgcta tggaaaaaga gcaatggaat gacacgcgta atctgcgcca gaaagtgaat
    16801 aaacgcactg aaaaagagtg ggataaagcc gacgctgctt ttgataaccg cgataaatgt
    16861 gagcaaagcg ccaacatcaa tgcctactgg gagccaaata ctttgcgctg cctggaccgt
    16921 cgaactggcc gcgttattat cccctaacct gttattgatt taaggaatgt aaggacacgt
    16981 tatgccaagc gcccacagtg ttaagctacg cccgctggag cgtgaagatt tacgctatgt
    17041 acatcaactc gacaataacg ccagtgtgat gcgttactgg tttgaggaac cctacgaagc
    17101 ctttgttgaa ctctctgatc tgtatgataa gcatattcac gatcagagcg aacggcgctt
    17161 tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa ttaaccatgt
    17221 tcatcgccgc gcagaatttc agataattat ctccccggag tatcagggga aaggtctggc
    17281 aacccgtgcc gccaaattag caatggacta tggcttcacc gttctcaatc tctataagct
    17341 gtatctgatc gttgataaag agaatgaaaa agcgattcac atttaccgca agcttggctt
    17401 tacggttgaa ggtgaattga tgcatgagtt ctttattaat ggtcaatatc gtaatgccat
    17461 tcgcatgtgt atattccagc atcagtatct ggctgagcac aaaacaccgg gtcagactct
    17521 cctgaagccg accgcacaat agtattaata ataatcgatc gtatttttga tggtgtaaac
    17581 ccgttcgacg gcgggtttta ctccttcatc aacaatgatt agctgacagt ccaccggatt
    17641 ggcgtgattg tcatattcac agctctgttt tacatttcca acccgttgat tattcagtaa
    17701 agtaaccgct gtgtaatcta attttttgat cgggtccgtt gatggcgtgg cgctgacaga
    17761 taatgtttta tcgttacttt tcgtggtttt acccagcgga tacccctgat catcatagcg
    17821 atattccatc tgcatctgtt tgctgctggc tttaatcacg aaaccattat catcggtttc
    17881 ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgca aacgaactct
    17941 tttctccagc gtctcggcat cacggtaata attggcgtcc agtaccaggg caaccacggt
    18001 attattttcc agatccagta attcgagaga atcaaaacag ccttcttccg acaaagtccc
    18061 cgaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga aatcttttac
    18121 gggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct ccggggtaaa
    18181 tgaaagtgtt acctctgtgc ggtcacatcc tgtgagtaat atcgcgagta agcatggcag
    18241 taatttgtat ttcacaacag tcacctgaga gtagagatga ttctcaatca tagtagcaaa
    18301 tacagtactt tacacgttaa atgctatgct taaagaagtt atcttcgcgt aaggagctta
    18361 tgatgaaact ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca cccgcggtac
    18421 tcgccgcgcc gggatcctgt gagcgcatac agagcgatat atcacagcgc attatcaata
    18481 atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccag gttgatcagc
    18541 ctgattccca ggtcgtcggg cattgcgcta atgatacgca taaaattctc tacacccgta
    18601 ccaccagcgg taacgtctct gctcccgcgc agtctaccca ggataacgcg cctgctgaac
    18661 cgcagtaaca cattatcgac tgaacgccgg atacgacaca tccggcgatt tgaacataca
    18721 acat
//
LOCUS       DADTFG010000095        18344 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.095, whole genome shotgun sequence.
ACCESSION   DADTFG010000095 DADTFG010000000
VERSION     DADTFG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.095"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..571)
                     /gene="rsmG"
                     /locus_tag="J1138_003064"
     CDS             complement(<1..571)
                     /gene="rsmG"
                     /locus_tag="J1138_003064"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBA8454831.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQV"
     gene            complement(635..2524)
                     /gene="mnmG"
                     /locus_tag="J1138_003065"
     CDS             complement(635..2524)
                     /gene="mnmG"
                     /locus_tag="J1138_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBA8454832.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2903..3346)
                     /gene="mioC"
                     /locus_tag="J1138_003066"
     CDS             complement(2903..3346)
                     /gene="mioC"
                     /locus_tag="J1138_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBA8454833.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3436..3894)
                     /gene="asnC"
                     /locus_tag="J1138_003067"
     CDS             complement(3436..3894)
                     /gene="asnC"
                     /locus_tag="J1138_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBA8454834.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4046..5038
                     /gene="asnA"
                     /locus_tag="J1138_003068"
     CDS             4046..5038
                     /gene="asnA"
                     /locus_tag="J1138_003068"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBA8454835.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5043..6494)
                     /gene="viaA"
                     /locus_tag="J1138_003069"
     CDS             complement(5043..6494)
                     /gene="viaA"
                     /locus_tag="J1138_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBA8454836.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKMIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6488..7984)
                     /gene="ravA"
                     /locus_tag="J1138_003070"
     CDS             complement(6488..7984)
                     /gene="ravA"
                     /locus_tag="J1138_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBA8454837.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8207..10075
                     /gene="kup"
                     /locus_tag="J1138_003071"
     CDS             8207..10075
                     /gene="kup"
                     /locus_tag="J1138_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBA8454838.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10242..10661
                     /gene="rbsD"
                     /locus_tag="J1138_003072"
     CDS             10242..10661
                     /gene="rbsD"
                     /locus_tag="J1138_003072"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBA8454839.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAGSQAVIRSGECSPYANIILCAGVTF"
     gene            10669..12174
                     /gene="rbsA"
                     /locus_tag="J1138_003073"
     CDS             10669..12174
                     /gene="rbsA"
                     /locus_tag="J1138_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBA8454840.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKLQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12179..13144
                     /gene="rbsC"
                     /locus_tag="J1138_003074"
     CDS             12179..13144
                     /gene="rbsC"
                     /locus_tag="J1138_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBA8454841.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13169..14059
                     /gene="rbsB"
                     /locus_tag="J1138_003075"
     CDS             13169..14059
                     /gene="rbsB"
                     /locus_tag="J1138_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HBA8454842.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKVGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14185..15114
                     /gene="rbsK"
                     /locus_tag="J1138_003076"
     CDS             14185..15114
                     /gene="rbsK"
                     /locus_tag="J1138_003076"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBA8454843.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15118..16110
                     /gene="rbsR"
                     /locus_tag="J1138_003077"
     CDS             15118..16110
                     /gene="rbsR"
                     /locus_tag="J1138_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBA8454844.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16076..17503)
                     /locus_tag="J1138_003078"
     CDS             complement(16076..17503)
                     /locus_tag="J1138_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBA8454845.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYAHKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(17526..18218)
                     /locus_tag="J1138_003079"
     CDS             complement(17526..18218)
                     /locus_tag="J1138_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBA8454846.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAKVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4399 a         4555 c         4808 g         4582 t
ORIGIN      
        1 gaacctgaag tttaaccact gattcgacct gatattcctc gggcaacaaa gcgatttcat
       61 cttccggcat ttgccctttc agcgcgtaga aacggccttg ctcaccagga agatggtggc
      121 accagctcac catatcgttc agagaggcaa aagcgcggct aattacgcca tcaaatggcg
      181 gctctgacgg aaactcttct accctgctct gtactggctc aatattctcc agtttaagct
      241 catgttgcac ctgacgaagg aaacgcacgc gtttaccaag gctatccaac aacgtgaaat
      301 gagcttcagg acgcacgata gagagtggaa tgccaggcag tcccggtccg gtgccgacat
      361 cgataaaccg ttcaccttgc agatacggtg ccaccacaat gctatcgaga atatggcgta
      421 ccagcatctc attaggatcg cggaccgaag tcaggttgta cgctttattc catttatgca
      481 gcatattcac gtaggcaata agctggtttt tctggtgatc ggtaagcgaa atacctgcgt
      541 ctttcagcag taaggagagt ttgttgagca cggtgattac ctgttcttga tgcgttgcct
      601 ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc gctacgacgc agcataccct
      661 gttttttcag ccacaccagc agaatggaga tagccgcagg cgtgacgcca gaaatacgcg
      721 aagcctggcc aatagaggcc ggtttgtgat cgttaagttt ggcgatcact tcgttagaaa
      781 gaccggatac ctggcggtaa tccagcgtcg ctggtagcag ggtattctcg ttacgcagct
      841 gcttttcgat ctcatcttgc tggcgcgcga tataaccttc gtatttaacc tgaatctcaa
      901 cctgttccgc agcctgttca tctgtcaacg caggggcaaa cggtgtcagc gtggttaatt
      961 tttcataagt catttcagga cgacgtagca gatcttcacc actggcttca cgggaaagcg
     1021 gcgcagtcag gtgagcattc acttcggctg cagcttccgc cgacggggtt acccaggtcg
     1081 atttcagacg ctgacgctca cgctcgatat tctcaagttt ctcgttaaag cgcgcccaac
     1141 gttcgtcatc caccaggccc agttcgcgac cgatttcagt caaacgcaga tcagcattat
     1201 cttcgcgcag catcagacga tattctgcgc gcgaggtaaa catacgatac ggttctttgg
     1261 ttcctaaagt gcacaggtca tcaaccagta cgccgagata tgcctgagaa cgcgccggag
     1321 cccaaccttc tttgtcagca gacagacggg cagcgttaag accagccagc aaaccttgcg
     1381 cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc agcaaagaac agcccctgga
     1441 taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc gaagaagtca tactcaatgg
     1501 cataacccgg acgcacgatc ttcgcgtttt ccatcccctg catggagcgg acgatttgca
     1561 tctgcacatc gaacggcagg ctggtggaga taccgttcgg ataaatttcg tttgaggtca
     1621 gcccttccgg ttcaaggaag atctgatgct gatttctgtc ggcgaagcgc atgactttgt
     1681 cttcgatcga cgggcagtag cgtgggccga caccttcgat cacccctgca tacattgggc
     1741 tacgatcgag gttactgcgg atcacatcat gggttttctc gttggtatgg gtgatataac
     1801 acggcacctg ctgtggatgc tgggacgcat tacccataaa cgagaatacc ggcattgggt
     1861 tatcgccatg ctgttgcgcc agcacgctga agtcgatagt acgtgcatca atacgcggcg
     1921 gtgtcccggt tttcagacga ccaacgcgca gcggcagttc acgcaaacgg cgagagagcg
     1981 gaatggacgg cggatcacca gcacggccac cgctgtaatt atccagaccg atatgaattt
     2041 taccgtcgag gaacgtccca acggtgagca cgacagcttt ggcacggaac ttcagtccca
     2101 tttgggtaac ggcaccgacc acgcgatcgt tttcgacaat aagatcttca accgcctgct
     2161 ggaagatcat caggttcggt tggttctcca gcgccgtacg taccgcctga cggtagagca
     2221 cacgatccgc ttgagctcgg gtagcgcgaa ccgccggtcc cttgcttgcg tttagtatcc
     2281 taaactggat acccgcctga tcgatcgctt tcgccatcag accgccgagt gcatccactt
     2341 cttttaccag atgtcccttc ccaataccgc cgatcgccgg gttgcagctc atctgcccca
     2401 gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc catacgcgcc gcggccatcg
     2461 cggcctcggt gcctgcatga cccccgccaa tgatgatgac gtcaaaagga tccggataaa
     2521 acatggtgat tgcctcgcat aacgcggtat gaaaatggat tgaagcccgg gccgtggatt
     2581 ctactcaact ttgtcggctt gagaaagacc tgggatcctg ggtattaaaa agaagatcta
     2641 tttatttaga gatctgttct attgtgatct cttattagga tcgcactgcc ctgtggataa
     2701 caaggatccg gcttttaaga tcaacaacct ggaaaggatc attaactgtg aatgatcggt
     2761 gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc aaaaactgaa
     2821 caaccgttgt tctttggata actaccgttt gatccaagct tcctgacaga gttatccaca
     2881 gcagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc agccattctt
     2941 ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt tcgcccgtct
     3001 gttttgcacc ggaatttttg agttcagcct cgagtttatc aatagcccca caaaaggtgt
     3061 catattcacg actaccaata ccgattgcgc caaagcggac cgcagaaaga tcgggcttct
     3121 gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg
     3181 tggagctgat aaccagccag atccccgagg cagataaatc ttccaacagc ggaccgtgca
     3241 gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct gctacatatt
     3301 cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac cgccaccttt
     3361 attaagagtg gcgtattgta cgctgtgaaa gcgttgggat ctacctgtgg aaaagtatgg
     3421 gattaaaaaa gccgatcagg gcttaatggt acgcatgatc gggttctgca ggacgatcag
     3481 tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta catgctggag
     3541 agcatcgatc gaacggcaca tcacttttat aaagatgctg tagtggccgg ttgtgtaata
     3601 ggcttcagtg acttcatcga ggctttccag ctttgccagc gcggaaggat agtctttggc
     3661 gctctttaat ataatgccga taaagcagcc tacgtcataa ccaagctgct tcgggctgac
     3721 atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc gaacgtgaat
     3781 cgtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc gcgcattgcc
     3841 cattaatgct tccaggatgc cacggtccag attgtcgatt agataatttt ccataggatt
     3901 ttcttatgcg gattgatgat tcattctatt ttagccttct tttttaatga atcaaaagta
     3961 cgtgaggctt tttattgaat gattattgca tgtgtgtcgg tttttgttgc ttaatcataa
     4021 gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac aacgtcaaat
     4081 tagcttcgtg aaatctcact tttctcgtca actggaagaa cgtctggggc tgatcgaagt
     4141 ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt cgggctgtga
     4201 aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc cagtttgaag tggttcattc
     4261 actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg gcgaagggct
     4321 gtacacgcac atgaaagccc ttcgccccga tgaagaccgt ctttccccgc tgcactcggt
     4381 ctatgtagac cagtgggact gggaacgcgt aatgggcgac ggcgagcgtc aattctcgac
     4441 tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag ctgcggttag
     4501 cgaagagttt ggcctggcac cgttcctgcc ggatcagatc catttcgtac acagccagga
     4561 gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga ttgcgaaaga
     4621 tcttggcgcg gtattcctcg tcgggattgg cggcaagttg agtgatggtc atcgccacga
     4681 cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggcc atgcgggtct
     4741 gaacggcgat attctggtat ggaacccggt actggaagat gcgtttgagc tttcttccat
     4801 ggggatccgc gtggatgccg acacgctgaa gcatcagctg gcgctgaccg gtgacgaaga
     4861 tcgcctgcag ctggagtggc atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg
     4921 cggcggtatc gggcagtctc gtttgaccat gctgctgctg caactgccac atatcggcca
     4981 ggttcagtgt ggtgtatggc cggcagctgt tcgtgagagc gtcccttctc tgctgtaata
     5041 atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata
     5101 tgatcgaaaa tgcgcatgat gccgggtttg ccatgtgccg acatcgccac ggcatgaaag
     5161 cgatgctgat gtacccgctg caactctttc actttgctcg tcacgtcgtc aggcaaccgc
     5221 tgggcgataa aatcagaaat caccaccgca tcggcatcaa accattcccg gctttgcaag
     5281 cgttccataa tggcgcgaaa acaactggcc agatcggtgc cgccacgaaa ctgctggctt
     5341 aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa gctcataacg gacgatctcg
     5401 gtggaaaata gcataatata gcagcgccgg ttttcagcga gggctatgcg catcaaggcc
     5461 aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga agtatccacg
     5521 cagacaataa acggtccgcg cggctgttcg tcgtaatctt tatgcaccac cgggcgttcg
     5581 atcatttttt cacgccacga ctcaccatgc aggcgatagg tgagcaactg tttttccacc
     5641 agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag ttctggcggc
     5701 aagagacgta aaatatcatc gctttgctgc agaccatcaa cctgctcagg aactgtcgcc
     5761 ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat cgttgcgcgg tattgatttg
     5821 gcttcccggg aacgccccag ctgttctgcc agacgtttca gttccggctg ttcgttaaga
     5881 aattcaccgt atttcacaat caactgatag tcgccacgtt taagctgacc ggcgctcata
     5941 tcccacagac gaccagctgc ggtattgtta tctgcgagaa tcggttcaag ttgcccgctc
     6001 agcgtcatgc gttcctgaac ttcactcaac agttgttcgc gttcttcttc taatagctgc
     6061 tgatttaacg tcgttgcttg cacgatcagg cttaaacgcc agcgctggag aaaaagcgtg
     6121 tgtaacgctg aagtgatcgt gctgttagca tcaaccaact ggcgggcttg ttctgcccat
     6181 ggggaattca gacgatgcag taagtccagg atttgtggta gctgcacaat aaactgcggc
     6241 gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc cggcgggact
     6301 cgggcatctt tcagccgact gcgcagcgcc tcacgccagc ggggaacatc atcagtgatt
     6361 gccgccttca gtcgtgggaa tttttcaaag aagactgcca gctgcggtga ggccagcagc
     6421 gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac attaagcgta
     6481 tccagcgtta gcattgttgt gcctggcgaa tctgtgcgcc gacatcctgt aggctggctt
     6541 caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg ctgaataaag
     6601 cgtgttgctt gcgccagtcg ctttcaagct cttccagttg ttgttttatt tcacctggca
     6661 gaccttcagc cgatgaacct ggaagtgcta gcgtactgcc ttgtaaactt acatcgcgta
     6721 caacaagatg ttgggcgcta tcgacttcca gattcagttt ctgggcaaag ccgataccgt
     6781 tcagtttgcc gcgaatctca ccacctttac tcagccactg ttccagcgcg ctacgctcaa
     6841 aggagatatg aaccacttcc atatcatgca gttttaacgg tttttgcagc agcagagtca
     6901 gagtggaagc agtaacgtta acagggagtt gatactgctg acgacggcta aaaatgccgc
     6961 ccagacgaat taccgttaag gctgttttat cgctttgttg ctgctgaagt tgcaggtgac
     7021 gttgcacaat cgcgcccagg cgggtcaaca tcccttgctg ttgccaggcg tgaccggtca
     7081 tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat
     7141 ctttcagcaa aatgagatca accggggcga cagcactgcg accgctaaaa aaggcgctgg
     7201 cctgcaataa gcggatcgct tttttccagc gacgatccga gacataaggc gcatccggta
     7261 atttatccag ttgctggcgc agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa
     7321 tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgatctgc aaggaggcag
     7381 gaaccggatt gtcattttca tcctgttgac tggtcagcat ggagcggaaa ttcgctttat
     7441 cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct tccagactgc
     7501 tgtctgcttc cggcagctcg ttggaggccg ccaccagcag acgcatcggg attttttcta
     7561 cgagtgcgcc gttgcggaac tggcgctcgt taatggcggt gagcagggta ttaagaattg
     7621 ccggacccgc tttccagatc tcatccagaa agacgatttc agcttccggc aggtaaccgc
     7681 tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa
     7741 cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca ttctgaaagg
     7801 cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggtgggcca aggaggaaca
     7861 cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta cgttcataaa
     7921 gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa tgagggtgag
     7981 ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt atagacgttt
     8041 cttgctggtg gctaaaatag tctcaaagga ggggtatttt tctttgagcc aggttaatgt
     8101 ggccgcattt aggagtacga ttttgccgtt aatcgtgcat actgtgcgct tttttgtggg
     8161 ccaagggact aagcacacat ttcatatttc aacgaaagac tagtctatga gcactgataa
     8221 taagcaatca ttgcccgcga ttaccctcgc ggcgattgga gttgtctacg gcgatattgg
     8281 taccagcccg ttatatacac ttcgtgaatg tttgtccggc cagtttggtt ttggcgttga
     8341 acgcgatgcc gtatttggct ttttatcgct gatcttctgg ctgctaatct ttgtggtttc
     8401 cattaaatat ctcaccttcg tgatgcgggc agataacgcc ggtgaagggg ggatcctgac
     8461 gttgatgtcg cttgccgggc gtaatacgtc ggcgcgaacc acatcaatgc tggtgattat
     8521 ggggctaatc ggcggcagct ttttctatgg tgaagtcgtc ataacacccg ctatttcggt
     8581 gatgtccgcc attgaaggtc tggaaatcgt cgccccgcag ctggatacct ggatagttcc
     8641 cctctcaatt atcgttctca cattattatt catgattcaa aaacatggca cggctatggt
     8701 cggtaagctg tttgcgccga tcatgctgac ctggtttttg attctggcag ggctggggtt
     8761 acgtagcatt attgctaacc cggaagtgct gcatgcactg aatccgatgt gggcggtgca
     8821 tttcttcctt gaatacaaaa cggtttcttt tattgcatta ggggcagtgg tgctgtcgat
     8881 tacgggggtc gaggcgctgt atgctgatat ggggcacttt ggtaagttcc ctattcgtct
     8941 ggcgtggttc accgtcgtat tgccttcctt aacccttaat tacttcggcc agggagcgct
     9001 gttgttaaag aatccggaag cgattaagaa cccgttcttc ctgttggcac cggactgggc
     9061 gctgatcccg ctgctgatca tcgccgcact ggcgacggta attgcctcgc aggcggttat
     9121 ctctggcgtc ttctcattga cgcgtcaggc ggtacgtctg ggatatttgt cgccgatgcg
     9181 cattattcac acttccgaaa tggagtcagg gcaaatctat attccctttg tgaactggat
     9241 gctctatgtc gcggtcgtga ttgtgattgt cagctttgag cactccagca acctggcggc
     9301 ggcgtacggg attgcggtga ccggaaccat ggtgctgacg tctattctct cgactaccgt
     9361 ggcacgtcag aactggcact ggaataagta ttttgttgcg ctgatcctga ttgctttcct
     9421 ttgtgtcgat attcccttgt tcaccgctaa cctcgataaa ctgctctccg gcggctggtt
     9481 gccattgagc ctcggtacgg tgatgtttat cgtgatgacc acctggaaga gcgagcgttt
     9541 ccgcttgctg cggcggatgc atgaacatgg taactctctg gaagcgatga ttgcttcgct
     9601 ggagaaatca ccgcccgttc gcgtgcctgg gaccgcggtg tatatgtcgc gtgcaatcaa
     9661 cgtcattccc tttgcgctga tgcataacct gaaacataac aaggtattgc atgagcgggt
     9721 gattctgtta actctgcgca ccgaagacgc gccatatgtc cataacgtcc gtcgggtaca
     9781 gattgaacaa ctgtcgccca ctttctggcg cgtggtggca agttatggtt ggcgagaaac
     9841 gccaaacgtg gaagaagttt tccaccgctg cggtctggaa ggattaagtt gccggatgat
     9901 ggaaacctcc ttctttatgt cgcatgagtc gttgatcctc ggcaaacgcc cgtggtattt
     9961 gcgtctgcgc ggcaagctgt acttgctgct gcaacgtaat gcgctgcggg caccggatca
    10021 atttgaaatc ccgccaaaca gagttatcga actgggtact caggtcgaaa tctaacgcca
    10081 gacgcctcct ttctccataa gggggcgttt ttgttttcat ggttaatcac catgtaaaac
    10141 gtttcgaggt tgatcacatt tccgtaacgt cacgatggtt ttcccaactc agtcaggatt
    10201 aaactgcggg tcagcgaaac gtttcgctga tggagaaaaa aatgaaaaaa ggcaccgttc
    10261 ttaattctga tatttcatcg gtgatctccc gactgggaca taccgatacg ctggtggtgt
    10321 gtgatgctgg tttacccatc cccaaaagta caacgcgtat cgatatggca ttaacccagg
    10381 gtgtaccttc ttttatgcag gtgctgggtg tagtcacaaa tgaaatgcag gtcgaggctg
    10441 tcattatcgc ggaagagatc aaacaacata atccgcaact ccacgaaacg ttgctcactc
    10501 accttgagca gctgcaaaaa caccagggaa acaccattga aattcgttac accacgcatg
    10561 aacaattcaa acaacaaacc gcaggaagtc aggcggtaat tcgcagcgga gaatgttctc
    10621 cgtatgcgaa tatcattctc tgtgctggcg tgacgttctg aggccgtcat ggaagcatta
    10681 cttcagctta aaggcatcga taaagccttc ccgggcgtaa aagccctctc gggcgcagcg
    10741 ttaaatgtct atccgggccg cgtgatggcg ctggtgggcg aaaacggcgc gggtaaatcc
    10801 accatgatga aagtgcttac tggtatctat actcgcgatg ccggtacgct tttatggttg
    10861 gggaaagaaa cgacatttac cgggccgaaa tcttcccagg aagccgggat tgggattatc
    10921 caccaggaac tgaacctgat cccgcagtta accattgccg aaaacatttt cctcggtcgt
    10981 gagtttgtta atcgctttgg caaaattgac tggaaaacca tgtatgccga agcggataaa
    11041 ttgctggcta aacttaacct gcgctttaaa agcgacaagc tggtgggcga tctttccatc
    11101 ggtgaccagc aaatggttga aatcgccaaa gtgctgagct ttgagtcgaa agtcatcatt
    11161 atggatgaac cgaccgatgc gctgaccgat accgaaaccg aatccttgtt ccgcgtcatc
    11221 cgcgagctga aattgcaagg ccgcggtatt gtctatatct cccaccgcat gaaagaaatc
    11281 ttcgagattt gcgatgacgt taccgttttt cgtgacggac aatttattgc tgagcgcgaa
    11341 gtggcatcac tgaccgaaga ttctctgatt gagatgatgg tgggccgcaa gctggaagat
    11401 caatatccgc acctggacaa agcgccggga gatatccgcc tgaaagtcga taatctctgc
    11461 ggacctggcg ttaacgatgt ctcttttact ttacgcaaag gcgaaattct tggcgtctct
    11521 ggtttgatgg gcgctgggcg taccgaactg atgaaagtgc tctacggtgc actgccgcgc
    11581 acaagcggtt acgtcaccct ggatgggcat gaagtcgtta cccgttcgcc gcaggatggc
    11641 ctggcaaacg gcattgttta tatctccgaa gaccgtaaac gtgacggttt agtgctgggc
    11701 atgtcagtaa aagagaacat gtcgctgacc gcgctgcgct acttcagccg cgctggcggc
    11761 agtttgaagc atgccgatga acagcaggct gtgagtgatt tcattcgtct gtttaatgtg
    11821 aaaacgccat cgatggaaca ggcaattggt ctgctttccg gtggcaatca gcaaaaagta
    11881 gcgattgccc gtggtctgat gacacgccct aaagtgttga tcctcgatga gcctacccgt
    11941 ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga ttaaccagtt caaagccgat
    12001 ggcttgagca tcattctggt gtcatcggag atgccagaag tattaggcat gagcgatcgc
    12061 atcatcgtca tgcatgaagg gcatctcagc ggggaattta ctcgtgagca ggccacccag
    12121 gaagtgttaa tggctgccgc tgtgggcaag cttaatcgcg tgaatcagga gtaaaaaaat
    12181 gacaacccag actgtctctg gtcgccgtta tttcacgaaa gcgtggctga tggagcagaa
    12241 atcgcttatc gctctgctgg tgctgatcgc gattgtctcg acgttaagcc cgaacttttt
    12301 caccatcaat aacttattca atattcttca gcaaacctcg gtgaacgcca ttatggcggt
    12361 cgggatgacg ctggtgatcc tgacgtcggg tatcgacttg tcggtaggtt ctctgttggc
    12421 gctgaccggc gcagttgctg catctatcgt cggcattgaa gtcaatgcgc tggtggctgt
    12481 cgctgccgct ctcgcgttag gtgccgcaat tggtgcggta actggggtga ttgtagcgaa
    12541 aggtcgcgtc caggcgttta tcgctacgct ggttatgatg cttttactgc gcggcgtgac
    12601 catggtttat accaatggta gcccagtgaa taccggcttt actgagaacg ccgatctgtt
    12661 tggctggttt ggtattggtc gtccgctggg cgtaccgacg ccagtctgga tcatggggat
    12721 tgtcttcctc gcggcctggt acatgctgca tcacacgcgt ctggggcgtt acatctacgc
    12781 gctgggcggc aatgaagcgg caacgcgtct ttctggtatc aacgtcaata aaatcaaaat
    12841 catcgtctat tctctttgtg gtctgctggc atcgctggcc gggatcattg aagtggcgcg
    12901 tctctcctcc gcacaaccca cggcggggac tggctatgag ctggatgcta ttgctgcggt
    12961 ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc attgttggga cgttgatcgg
    13021 cgcattaatt cttggcttcc ttaataatgg attgaatttg ttaggtgttt cctcctatta
    13081 ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg ctggtagaca acaaaaagca
    13141 gtaataacga ctacaggaca tcttgaatat gaacatgaaa aaactggcta ccctggtttc
    13201 cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg gcaaaagaca ccatcgcgct
    13261 ggtggtctcc acgcttaaca acccattctt tgtatcgctg aaagatggcg cgcagaaaga
    13321 ggcggataaa cttggctata acctggtggt gctggactcc cagaacaacc cggcgaaaga
    13381 gctggcgaac gtgcaggact taaccgttcg cggcaccaaa attctgctga ttaacccgac
    13441 cgactccgac gcagtaggta atgctgtgaa gatggctaac caggcgaaca tcccggttat
    13501 cactcttgac cgccaggcaa cgaaaggtga agtggtgagc cacattgcat ctgataacgt
    13561 actgggcggc aaaatcgccg gtgattacat cgcgaagaaa gtgggtgaag gtgccaaagt
    13621 tatcgagctg caaggcattg ccggtacatc cgcagcccgt gaacgtggcg aaggtttcca
    13681 gcaggccgtt gctgctcaca agtttaatgt tcttgccagc cagcctgcag attttgatcg
    13741 caccaaaggt ttgaacgtaa tgcagaacct gttgaccgct catccggatg ttcaggctgt
    13801 attcgcgcag aatgatgaaa tggcgcttgg cgcgctgcgc gcactgcaaa ctgccggtaa
    13861 atcggatgtg atggtcgtcg gatttgacgg tacaccggat ggcgaaaaag cggtgaatga
    13921 tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga aaggcgtcga
    13981 aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg ttgatctgaa
    14041 actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac ataaatacgt
    14101 catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc taacacggtg
    14161 gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc
    14221 attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga aaccgtaacc
    14281 ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggcggt ggctgctggg
    14341 cgtagcggtg cgaatatcgc gtttattgcc tgcactggcg atgacagcat tggtgagagc
    14401 gttcgccagc agctcgccac tgataacatc gatatttctc cggtcagcgt gatcaaaggc
    14461 gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt catcggtatt
    14521 catgccggcg ctaatgctgc cctttccccg gcgctggtgg aagcgcaacg tgagcgtatt
    14581 gccaacgcgt cagcattatt aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg
    14641 gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc ggctcgcgaa
    14701 cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac ggaagcagaa
    14761 aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg
    14821 catgaaaaag gtatccgtac tgtactgatt actttaggaa gtcgtggtgt atgggctagc
    14881 gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga tactattgct
    14941 gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa accattgcca
    15001 gaggcgattc gttttgccca tgcagccgct gcgattgccg taacacgtaa aggcgcacaa
    15061 ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag gtgacgcttg
    15121 gctacaatga aagatgttgc ccgcctggcg ggcgtttcta cctcaacagt ttctcacgtt
    15181 atcaataaag atcgcttcgt cagtgaagcg attacagcca aagttgaagc ggcgattaaa
    15241 gaactcaatt acgcgccatc agctctggcg cgtagcctca aactcaatca aacacatacc
    15301 attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt gcgtggcgtt
    15361 gaacgcagct gcttcgaacg cggttatagt cttgtccttt gcaataccga aggcgatgaa
    15421 cagcggatga atcgtaatct ggaaacgctg atgcaaaaac gcgttgatgg cttgctgtta
    15481 ctgtgcaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct
    15541 accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca ggataactcg
    15601 ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac ccgtattgcc
    15661 tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg ttatcgggct
    15721 gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg tgattttgaa
    15781 tttaacggcg ggtttgacgc tatgcgccaa ctgttatcac atccgctgcg tcctcaggcc
    15841 gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata tcaggcagag
    15901 ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact ggcaagcttt
    15961 atgacgccac cattaacaac tatacaccaa ccgaaagatg aactggggga gctggcgatt
    16021 gatgtactca tccatcggat aacccagccg accctacagc aacaacgatt acaacttact
    16081 ccgattctga tggaacgtgg ttcggcttag atttacgctg tcttttgatc aaattattac
    16141 catcggttgt tttcagtagc atgaacattg ctgctgaagc aacagtaata atgcccatcg
    16201 tgataaacgt atagtggaat tgttcgactg tcgttgtgcc ttccatccct tcgtaaacgc
    16261 gaaggactgc cgcacttaca gcaacgccta aactgatcga cagttgctgc gtgaccgcca
    16321 gaacactgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg atggtattca
    16381 tcgcggtaaa ttgtgtcgac atagccatcc ctaatataaa caacggcaag atcagcatcc
    16441 atatcgccat tgctggtgat tgcaaagaga actgagcgat cattagccct ataatcaccg
    16501 tgatccccac taacgtatgg cgatagccca gacgacgtaa gacttgggta accatcgatt
    16561 ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccagcaata aacgcctgat
    16621 acccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg gtccccagac
    16681 gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat aaatctaatg
    16741 aaattaatgg gtttggcgtg cgtcgcgcat ggagaatata gagaagcagt aacccgatgc
    16801 tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg aatagctcta
    16861 ttccgcttga gaagagaaca aggctgaggc caaacagcaa aaagccagtg gtatcgaatc
    16921 tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgtgcgc gtaaagaagg cccgcaatac
    16981 ctatggggat attgattaaa aatatccagt gccaggttgc ccaggtgacc agcacgccgc
    17041 caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa ttcaatacag
    17101 gaagaagctc attacgagga taagcgcgca gtaaggccag ccgagcaaca ggcatcatca
    17161 ttgcgccgcc tatcccctga ataacccgga agacaaccag ctgtggtagc gagttagaaa
    17221 gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaat atgcgacgcg
    17281 taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc gtcagcgtat
    17341 aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga gcgattgcgg
    17401 gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaagaaggcc atcgccgcga
    17461 tccacggcaa acccgccata ctgcgcttct ttttatcgct cattcaatgt cctgttatcg
    17521 ggttatcact tatctggtga gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca
    17581 tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat cactgtgtcg
    17641 ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc gaatgaggtc
    17701 aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggccatatc gacttcgatc
    17761 cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa cgtattgaga
    17821 tgcgccttct gttccgcggt gccaaccttt gctgccagta ggcaagcttg cggctccaga
    17881 cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc tgtcatccac
    17941 caggtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac tcgggtacca
    18001 attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc ttcgcgtacc
    18061 gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccaggcaa aatggtgccg
    18121 ggttcatatt caccttttaa gatccgctgc gccagcttct cagccagaac atacgaaagg
    18181 tttttctgtg cagctaactg ttgtgcgctt aaaggcatta ctaatcttcc tttttctttt
    18241 tattccttct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg gcaaattgct
    18301 tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcg
//
LOCUS       DADTFG010000096        18305 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.096, whole genome shotgun sequence.
ACCESSION   DADTFG010000096 DADTFG010000000
VERSION     DADTFG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.096"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            67..996
                     /gene="pfkB"
                     /locus_tag="J1138_003080"
     CDS             67..996
                     /gene="pfkB"
                     /locus_tag="J1138_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBA8454847.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEAQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1097..1387
                     /gene="ghoS"
                     /locus_tag="J1138_003081"
     CDS             1097..1387
                     /gene="ghoS"
                     /locus_tag="J1138_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBA8454848.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            1493..2353
                     /locus_tag="J1138_003082"
     CDS             1493..2353
                     /locus_tag="J1138_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBA8454849.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2394..2930)
                     /locus_tag="J1138_003083"
     CDS             complement(2394..2930)
                     /locus_tag="J1138_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454850.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNVFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PLICIAAGYVFFSLLGFI"
     gene            3077..3745
                     /gene="hxpB"
                     /locus_tag="J1138_003084"
     CDS             3077..3745
                     /gene="hxpB"
                     /locus_tag="J1138_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBA8454851.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3909..4499
                     /locus_tag="J1138_003085"
     CDS             3909..4499
                     /locus_tag="J1138_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA8454852.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIKFHRLIKHQVEY"
     gene            4632..6023
                     /locus_tag="J1138_003086"
     CDS             4632..6023
                     /locus_tag="J1138_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBA8454853.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6070..6333)
                     /gene="cedA"
                     /locus_tag="J1138_003087"
     CDS             complement(6070..6333)
                     /gene="cedA"
                     /locus_tag="J1138_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBA8454854.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6516..8777
                     /gene="katE"
                     /locus_tag="J1138_003088"
     CDS             6516..8777
                     /gene="katE"
                     /locus_tag="J1138_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBA8454855.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLENVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILNALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADSADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            complement(8824..9582)
                     /gene="chbG"
                     /locus_tag="J1138_003089"
     CDS             complement(8824..9582)
                     /gene="chbG"
                     /locus_tag="J1138_003089"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBA8454856.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTVMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(9595..10947)
                     /locus_tag="J1138_003090"
     CDS             complement(9595..10947)
                     /locus_tag="J1138_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA8454857.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELNVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(11051..11890)
                     /gene="chbR"
                     /locus_tag="J1138_003091"
     CDS             complement(11051..11890)
                     /gene="chbR"
                     /locus_tag="J1138_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBA8454858.1"
                     /translation="MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYY
                     EFTLVLTGRYFQEINGKRMLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTE
                     FNQ"
     gene            complement(11901..12251)
                     /gene="chbA"
                     /locus_tag="J1138_003092"
     CDS             complement(11901..12251)
                     /gene="chbA"
                     /locus_tag="J1138_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBA8454859.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12302..13660)
                     /gene="chbC"
                     /locus_tag="J1138_003093"
     CDS             complement(12302..13660)
                     /gene="chbC"
                     /locus_tag="J1138_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBA8454860.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(13745..14065)
                     /gene="chbB"
                     /locus_tag="J1138_003094"
     CDS             complement(13745..14065)
                     /gene="chbB"
                     /locus_tag="J1138_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBA8454861.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14365..14703)
                     /gene="osmE"
                     /locus_tag="J1138_003095"
     CDS             complement(14365..14703)
                     /gene="osmE"
                     /locus_tag="J1138_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBA8454862.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            14905..15732
                     /gene="nadE"
                     /locus_tag="J1138_003096"
     CDS             14905..15732
                     /gene="nadE"
                     /locus_tag="J1138_003096"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBA8454863.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            15962..16849
                     /gene="cho"
                     /locus_tag="J1138_003097"
     CDS             15962..16849
                     /gene="cho"
                     /locus_tag="J1138_003097"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBA8454864.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(16809..17384)
                     /gene="ves"
                     /locus_tag="J1138_003098"
     CDS             complement(16809..17384)
                     /gene="ves"
                     /locus_tag="J1138_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBA8454865.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(17587..18072)
                     /gene="spy"
                     /locus_tag="J1138_003099"
     CDS             complement(17587..18072)
                     /gene="spy"
                     /locus_tag="J1138_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBA8454866.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         4698 a         4609 c         4310 g         4688 t
ORIGIN      
        1 tattgttaat ttcttcactt tccgctgatt cggtgccaga ctgaaatcag cctataggag
       61 gaaatgatgg tacgtatcta tacgttgaca cttgcgccct ctcttgatag cgcaacaatt
      121 accccgcaaa tttatcccga aggaaaactg cgctgtaccg caccggtgtt cgaacccggt
      181 ggcggcggca tcaatgtcgc ccgcgccatt gcccatctgg gaggcactgc cacagcgatc
      241 ttcccggcgg gtggcgcgac cggcgaacat ctggtttcac tgttggcgga tgaaaatgtc
      301 cccgtcgcta ctgtagaagc caaagactgg acccggcaga atttacacgt acatgtggaa
      361 gcaagcggtg agcagtatcg ttttgttatg ccaggcgcgg cattaaatga agatgagttt
      421 cgccagctcg aagcgcaagt tctggaaatt gaatccgggg ccatcctggt cataagcgga
      481 agcctgccgc caggtgtgaa gctggaaaaa ttaacccaac tgatttccgc tgcgcaaaaa
      541 caagggatcc gctgcatcgt cgacagttct ggcgaagcgt taagtgcagc actggcgatt
      601 ggtaacatcg agttggttaa gcctaaccaa aaagaactca gtgcgctggt aaatcgcgaa
      661 ctcacccagc cagacgatgt ccgcaaagcc gcgcaggaaa tcgttaatag cggcaaggcc
      721 aaacgggttg tcgtttccct gggtccacaa ggggcgctgg gcgttgatag tgaaaactgc
      781 attcaggtgg tgccaccacc ggtgaaaagc cagagtaccg ttggcgctgg tgacagcatg
      841 gtcggcgcga tgacacttaa actggcagaa aatgcctctc ttgaagagat ggtccgtttt
      901 ggcgtagctg cggggagtgc agccacactc aaccagggaa cacgcctgtg ctcccatgac
      961 gatacgcaaa aaatttacgc gtacctttcc cgctaacaaa aacattcccc cagcattggg
     1021 ggaatcacca ccaacctgtc ggcaacgcgt ttctccgact atgctcaaaa gtcatgtgat
     1081 aacaaagggg tgaactatgg ccagtggcga tcttgtccgt tatgtaataa ccgtcatgtt
     1141 gcatgaggat acattgactg aaattaacga gttgaataat tacctgactc gcgacggttt
     1201 tttgctcacc atgacggatg atgatggcaa tatccatgag ctggggacta acacttttgg
     1261 acttatcagt acccaaagtg aagaagaaat tagagaactg gtttcggggc ttacccacag
     1321 tgcaaccggc aaagatcctg aaatcaccat cacgacctgg gaggaatgga atagcaacag
     1381 aaaataaatg gtttttgggc aataatcagt ctgtggtgtg cgttagctcg tgtttttaca
     1441 ccgcattctt gcgctaacct tatgatctgg cagacaacat gggagagaca tcatgtggca
     1501 ggcaatcagt cgtcttttga gcgagcagtt aggtgaaggc gaaatcgaac tgcgtaatga
     1561 actgcctggc ggagaagtcc atgccgcatg gcatttgcgc tatgcaggac gtgacttttt
     1621 cgtcaaatgt gatgaaaggg aactgcttcc cggttttacc gccgaagccg accaactgga
     1681 gttactgtcg cgtagtaaaa ccgtcaccgt gcctaaggtt tgggcagttg gcgctgaccg
     1741 tgactacagt tttctggtga tggattatct cccacctcgc ccgctggatg cgcatagtgc
     1801 atttattctt ggtcagcaaa ttgcgcgttt acatcaatgg agcgaccaac cacaatttgg
     1861 cctcgatttc gataacgcgc tctccacaac tccacagccc aacacctggc aacgtcgctg
     1921 gtcaactttt tttgctgaac aacggattgg ctggcagctg gaactggcgg cagaaaaagg
     1981 gatcgctttc ggcaatatcg atgccatcgt cgagcatatt cagcagcgtc tcgcctcaca
     2041 tcaaccgcag ccttctctgt tgcacggcga tttatggtcc ggcaactgtg cgctgggtcc
     2101 ggatggcccg tacattttcg acccggcctg ttactggggt gaccgagagt gcgacctggc
     2161 gatgttaccg ctgcatactg aacagccgcc acaaatctat gacggctatc agtcagtatc
     2221 cccgctacct gccgatttcc ttgaacgtca accggtttac caactctaca cgctgttaaa
     2281 tcgtgcaagg ttatttggcg gtcagcattt ggttattgct cagcagtcac tggatagatt
     2341 attagcagca tgatatgggt tgaggataat ggccgctccg tgcggccttt tgattaaata
     2401 aacccaagca gagaaaagaa cacataacct gcagcaatac aaataagcgg caggatatac
     2461 agcgagaaaa actgcaggaa aatagtatgg tgcggaacaa caatacgtga ctcaatttgt
     2521 tcacgcgtta gcccctcttc ccctttggct ttttccagaa tgagttgatc ttcaacacct
     2581 tcccgcaaga agcgcgcctg gcgactcatc cgggctccgg aatcctgcaa cgccagtccg
     2641 acaaaaatca ggatgaaaat cacccagaac ataacgttca cgccaccatt aaaattgggc
     2701 gtcggagagt tgtaccagaa cacattcaaa aaaggcgtat tcgcctgcat catatcgatc
     2761 atgacatgag taaagtcgag catgaccgca ttaatgcctt cctggttttc ctgccgggta
     2821 ttcataaatt tcagcaaaga aatcaatgta gagatcaatg caggtataaa aatcacccaa
     2881 cccaaaatcc ttttcaaaac agcaatgcgt ccagcttgtt gatacgtcat gagttctcct
     2941 tgattaagac gcgtcatttc acttagttta cctgtagata tctgttttcg cccattcttt
     3001 aaaggcgata tgataggcgc ttaatcataa gcacggctta ataccttaca tataatgctc
     3061 taaaggagag gttgtaatgt caaccccgcg tcagattctt gctgcaattt ttgatatgga
     3121 tggattactt atcgactcag aacctttatg ggatcgagcc gaactggatg tgatggcaag
     3181 cctgggggtg gatatctccc gtcgtaacga gctgccggac accttaggtt tacgcatcga
     3241 tatggtggtc gatctttggt acgcccggca accatggaat gggccaagcc gtcaggaagt
     3301 agtagaacaa gttattgccc gtgccatttc actggttgaa gagacacgtc cattattgcc
     3361 aggtgtgcgt gaagccgttg cgttatgcaa agaacaaggt ttattggtgg ggctggcctc
     3421 cgcgtcaccg ctgcatatgc tggaaaaagt attgaccatg tttgatttac gcgacagttt
     3481 cgatgccctc gcctcggctg aaaaattgcc ttacagcaag ccgcatccgc aagtatatct
     3541 cgactgcgca gcaaaactgg gggttgaccc tctgacctgc gtggcactgg aagattcggt
     3601 aaatggcatg atcgcctcta aagcagcccg catgcgttcc atcgtcgttc ctgcgccaga
     3661 agcgcaaaat gatccacgtt ttgtattagc aaacgtcaaa ctctcatcgc tgacagaact
     3721 caccgcaaaa gaccttctcg gttaatgacc agggggcagt gatcacactg cccctggttc
     3781 tttatctgaa tttgcattca aatgacggat taatcgccaa tttaagagaa agagttacac
     3841 cgtcaccact tccgtgcact gtataaaaac cctatactgt acgtatcgac agtttagtga
     3901 gttttatcat gacggcggaa ggtcaccttc tcttttctat tgcttgtgcg gtatttgcca
     3961 aaaatgccga gctgacgccc gtgctggcac agggtgactg gtggcatatt gtcccttccg
     4021 caatcctgac gtgtttgtta ccggacatcg atcacccaaa gtcgtttctt gggcagcgat
     4081 taaagtggat atcaaaaccg atcgcccgcg cttttgggca tcgtggtttt acccacagtc
     4141 tgctggcggt atttgcgctg ctggcaacct tttaccttaa agttcctgaa agctggttca
     4201 ttccggctga tgcgttgcag ggtatggtgc tgggttattt gagccacata cttgccgata
     4261 tgctgacccc cgccggtgtt cccctgctct ggccatgccg ctggcgtttc cgcttgccta
     4321 tcctggttcc ccaaaagggc aaccaactgg aacgttttat ctgcatggca ttatttgtct
     4381 ggtcggtatg gatgccccat tcattacccg aaaacagcgc tgttcgttgg tcatcgcaaa
     4441 tgatcaatac cttacagatc aagtttcatc ggcttattaa gcatcaggtt gaatactaaa
     4501 aaggcaaaaa tcgcctttct ggaataagca attccatttg aatataagag ccagctcaca
     4561 gttctgttaa tcttgcgcca acactatgac tgctacgcag tgatagaaat aataagatca
     4621 ggagaacggg gatgaacttt ccattaattg cgaacatcgt ggtgttcgtt gtactgctgt
     4681 ttgcgctggc tcaaacccgc cacaaacagt ggagtctggc gaaaaaagtg ctggtgggtc
     4741 ttgtgatggg tgtggttttt ggccttgccc tgcataccat ttatggttct gacagccagg
     4801 tacttaaaga ttctgtacag tggtttaaca ttgttggtaa cggctatgtt caactgctgc
     4861 aaatgatcgt tatgccgcta gtcttcgcct ctattctgag cgcggttgcc cgtctgcata
     4921 acgcatctca gttaggcaaa atcagttttc tgaccatcgg tacgcttttg tttaccacgc
     4981 tgattgcagc gctggttggt gtgctggtca ccaacctgtt tggtttgacg gctgaaggtc
     5041 tggttcaggg tggtgcagaa actgcacgtc tgaacgccat cgaaagtaac tatgttggta
     5101 aagtctctga cctgagcgtt ccgcagctgg tcttgtcctt tatcccgaaa aacccgtttg
     5161 ccgatctgac cggagccaat ccgacgtcaa ttatcagcgt ggtaattttt gccgcattcc
     5221 tcggcgtagc tgcgctgaaa ctgctgaagg atgatgcgcc gaaaggtgaa cgcgtcttaa
     5281 ccgctatcga taccctgcaa agctgggtga tgaaactggt tcgcctggtc atgcagttga
     5341 ccccttacgg tgttctggcg ctgatgacca aagtggttgc aggttctaac ctgcaagaca
     5401 tcatcaaact gggaagtttc gttgttgcgt cctacctcgg tctgctgatt atgtttgcag
     5461 tgcatggcct tctgctgggc attaatggcg tgagcccgct gaagtacttc cgtaaggtat
     5521 ggccagtact gacgtttgcc tttaccagcc gttccagtgc tgcgtctatc ccactgaatg
     5581 tggaagcaca aacgcgtcgt ctgggtgttc ctgaatccat cgccagtttc gccgcctctt
     5641 tcggtgcgac cattggtcag aacggctgcg ccggtttgta tccggcaatg ctggcggtga
     5701 tggttgcgcc tacggtgggc attaacccgc tggacccgat gtggattgcg acgctggtcg
     5761 gtattgttac cgttagttcc gcaggcgttg ccggtgtcgg tggtggtgca actttcgccg
     5821 cactgattgt gctgcctgcg atgggcctgc ctgtaacgct ggtggcattg ttaatctccg
     5881 ttgaaccgct tatcgacatg gggcgtacgg cgctaaacgt tagtggctcg atgaccgctg
     5941 gcacgctgac cagccagtgg ctgaagcaaa ccgataaagc cattctggat agcgaagacg
     6001 acgccgaact ggcacaccgt taattctgat ttgttacgtt aaacgcctgt catcccgaca
     6061 ggtgtttttt tattcttcga cctcattttc ctgacgaatc tgatttgccc aacgctgggc
     6121 ggattcaggc acagtaaacg ccggtgagcg cagaaatgac tcccccatca gtacaaacgc
     6181 aacatatttg ccacgcagca tccagacatc acgaaacgaa tccatcttta tcgcatgttc
     6241 tggcggcgcg ggttccgtgc gtgggacata gctaataatc tggcggtttt gctgacggag
     6301 cggtttcttc attactggct tcactaaacg catattaaaa atcagaaaaa ctgtagttta
     6361 gccgatttag cccctgtacg tcccgctttg cgtgtatttc ataacaccgt ttccagaata
     6421 gtttccgaag cgggatctgg ctggtggtct atagttagag ggttttttga ccaaaacagc
     6481 ggccctttca gtaataattt aaggagacga gttcaatgtc gcaacataac gaaaagaacc
     6541 cacatcagca ccagtcacca ctacacgatt ccagcgaagc gaaaccgggg atggactcac
     6601 tggcacctga agacggatct catcgtccag cggctgaacc aacaccgcct ggtgcacaac
     6661 ctaccgcccc agggagcctg aaagcccctg atacgcgtaa cgaaaaactt aattctctgg
     6721 aaaacgtacg caaaggcagt gaaaattatg cgctgaccac taatcagggc gtgcgcatcg
     6781 ccgacgatca aaactcactg cgtgccggta gccgtggtcc aactctgctg gaagatttta
     6841 ttctgcgcga gaaaatcact cactttgacc atgagcgcat cccggaacgt attgttcatg
     6901 cacgcggatc agcagctcac ggttatttcc agccatataa aagcttaagc gatatcacca
     6961 aagcggattt cctctcagat acgaacaaaa tcaccccagt atttgttagg ttctctaccg
     7021 ttcagggcgg cgctggctct gccgataccg tacgtgatat ccgtggcttt gccaccaagt
     7081 tctatactga agagggtatt tttgacctcg ttggcaataa cacgccaatc ttctttatcc
     7141 aggatgcgca taaattcccc gattttgttc atgcggtaaa accagaaccg cactgggcaa
     7201 ttccacaagg gcaaagcgcc cacgatactt tctgggatta tgtttctctg caacctgaaa
     7261 ctctgcacaa cgtgatgtgg gcaatgtcgg atcgcggtat cccgcgcagt taccgcacta
     7321 tggaaggctt cggtattcat accttccgcc tgattaatgc cgaagggaag gcaacgttcg
     7381 tacgtttcca ctggaaacca ctggcaggta aagcctcact cgtttgggat gaagcacaaa
     7441 aattaaccgg acgtgacccg gacttccacc gccgcgagtt gtgggaagcg attgaagcag
     7501 gcgattttcc tgaatacgaa ctgggcttcc agttgattcc tgaagaagac gaattcaagt
     7561 tcgacttcga tcttctcgat ccaaccaaac ttatcccgga agaattggtg cccgttcagc
     7621 gtgtcggcaa aatggtgctc aatcgtaatc cggataactt ctttgctgaa aacgaacagg
     7681 tggctttcca tccagggcat attgtccccg gtctggattt caccaacgac ccgttattgc
     7741 aggggcgttt gttctcttat accgatacac aaatcagtcg tcttggtggg ccgaatttcc
     7801 atgaaattcc gattaaccgc ccgacctgcc cttaccataa tttccagcgt gacggcatgc
     7861 atcgtatggg gatcgacact aaccctgcga attacgaacc gaactcgatc aacgataact
     7921 ggccgcgcga aacaccgccg gggccgaaac gcggcggttt tgaatcatac caggaacgcg
     7981 tggaaggaaa taaagttcgc gagcgcagtc catcgtttgg cgaatattat tcccatccgc
     8041 gtctgttctg gctaagtcag acgccattcg agcagcgcca tattgtcgat ggtttcagtt
     8101 ttgagttaag caaagtagtt cgtccgtata ttcgtgagcg cgttgttgac cagctggcgc
     8161 atattgatct cactctggcc caggcggtgg cgaaaaatct cggtatcgaa ctgactgatg
     8221 accagctgaa tatcacccca cctccggacg tcaacggtct gaaaaaggat ccatccttaa
     8281 gtctgtacgc cattcctgac ggtgatgtga aaggtcgcgt ggtagcaatt ttgcttaatg
     8341 atgaagtgag atcggcagac cttctggcca ttctcaatgc actgaaggcc aaaggcgttc
     8401 atgccaaact gctctactcc cgaatggggg aagtgactgc ggatgacggt acggtgttgc
     8461 ctatagctgc tacctttgca ggtgcgcctt cgctgacggt cgatgcagtc attgtccctt
     8521 gcggcaatat tgcggatatc gctaacaacg gcgatgccaa ctactatctg atggaagcct
     8581 acaaacacct taaaccgatt gcgctggcag gagacgcgcg caagtttaaa gcaacaatca
     8641 aggtcgctga ccagggtgaa gaagggattg cggaagctga cagcgccgat ggtagtttta
     8701 tggatgaact gttaacgctg atgacagcac accgcgtgtg gtcacgcatt cctaagattg
     8761 acaaaatccc ggcgtaaata tggataaggt cacgccccct ggggagtgac cttaccacct
     8821 tacttacaca tcatgataac tacccaaccg ataaccacgc tcagcaatcg catatttcaa
     8881 cgacgctgaa gtcagcacat ccagttccgt taaccgaggg aagcaataag cactctggcg
     8941 aatcgtatta tcgacaaacg ccggatggca cataacttcc aacgagcgtt ccccacgatg
     9001 gctggaatca tcaagcactt gcaggaacaa tgcctcgcta atctcttcac cataaaacgc
     9061 gctactgaat ccctgcgaac tgcgcagatt agccggtaaa tcaccatcgt tagatagcgg
     9121 ttgacggtcg atgcgcaacg caatcccctc ttcagccgca aactttgcca caatcgggaa
     9181 aatctgcggg aacatatgca catgatgatg gctatcaaga tgcgtaggtt tgcgtccaaa
     9241 tagctcaatg aaacgcaaat actgactggc gagctcctga gtaatttctt ccagcggtaa
     9301 agcatcttct tctgccaact gccagatcca ttttcccagc acaccatcgc gggttaaccc
     9361 cggcataact gtcagaggct tgcccatagt aaggacaaag tgcatcccta tggccagact
     9421 cggttcatca cggctcaact gcaccgcatg gtcaatagcc tgcccattca ccagcgccgt
     9481 cgtcgacgtg acaatcccat tgcgacaggc ctcgataatg ccgtagttct gacctttgct
     9541 taagccaaaa tcatcggcat taacaatcag taagcgttcc ataatcagcc tcggttaatg
     9601 tgctttttta agctctgcga tgcagtcggc aaaatttggc agccatttct cgtgcgccag
     9661 aatcatctcg cgtgccagca gctcagcatc gcgatcggaa tgcaccaacg gactaaggtt
     9721 tagcgccagt aagacatcgt taaattctcc actaagtgcg gcgttgctgg cagcaatctc
     9781 gaagcctttg atggtgtgaa tcagccccat cactttatca tcgaaatgcg taatgcgcgg
     9841 atgtggcgtc gcgccatcgc gccccagcgt acaggtcatt tctaccgccc agtctgccgg
     9901 aatattatca atatgcccat gatgcgggat attaacgtaa tgttctgctt gcttgtcgtt
     9961 atagatagcg ttgatcactt cgcacgcagc atcagaataa taagccccac cgcgctgttc
    10021 cagttctttc ggcttaacgt tcaactccgg gtttttatat agctcaaaaa gttgtttctc
    10081 gactttctgt actacctgcg ctcgtgcgcc gcctttgtag tattcgccca tttcaatagc
    10141 cagcatctct ttttgcttga agtaataaag caaataagaa cacggcaaca gattcataga
    10201 acgaattaag ccttcgctaa atggcagatc gaaaatattt ttaacgccag atgcttttaa
    10261 ctgcccggat gccacaccat caagcaattc ggcaaagcgt gatttgccat ttaccagcac
    10321 atccttaatg aacaccatat ggttgaggcc gaacagatcg atagataaat catcgctgtc
    10381 tttcagcatc agaacatcgc gaataaacat cttcatgccg atcggaatat tacacacgcc
    10441 gataaagcgt ttaaatccgg tatgacgata aacggcttca gtgaccattc ccgccgggtt
    10501 agtgaagtta atcacccatg cattcggaca tagttcttcg acatctttta cgatgtcaaa
    10561 aatcaccgga atggtacgca gacctttaaa cagaccgccc gcgccgttag tttcctgacc
    10621 aagataacca tgacttaatg gaatacgttc atccagttcc cgcgccggta attggccaac
    10681 gcgcagttgg gtagtaacga aatcagcatc tttcaatgct tcgcggcgat ccagcgtttt
    10741 ataaagcttc atcgggacgc cagcgttatc aatcatccgt tggcagagat caaaaataat
    10801 atccagtttc gctttaccac cttcgacatc caccagccat aattcgctga ccggcaattc
    10861 gtgataacgc ttaataaatc cttccagtaa ctccggggta tagctgctcc cgccaccaat
    10921 agtgacgact tttaatttct ggctcataat ttctcccttc agtacagaat actgatatct
    10981 ggcatatctg ccccccggac ataaataatc cagcaacagg acagatatgt gaattgtcag
    11041 gtataacgac ttactgatta aattcagtca atttcttacg atagctctta ggcgtaaagg
    11101 acgttaattt cttaaacgtt ttaataaaca agctcgggct actataaccg gcctcaaacg
    11161 caatatccgt cactgaatag ttggtcattt ccagttgttt tttggcaaaa ttaatacgga
    11221 tttcattaat aatctgcatt ggcgttttac catagtatcg ttgagtcgct cgcgtcaaat
    11281 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
    11341 ctttatcatg catcttttct accgtacttt tcagccactg cggtatatca tcaatcacct
    11401 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
    11461 cttccagccc tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca taggtgagaa
    11521 aagcgttatt ggtccggtat acctgcgaag ctacaaagca ataaggcaac aacggcaggt
    11581 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg gcaccaaatt
    11641 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
    11701 acatgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
    11761 aatagtcgtg ctgatgcagt ccactgatac tctcagtttt attgtagata aacacatgga
    11821 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
    11881 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
    11941 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
    12001 ctcactttca tcttaccttc gccagcatcg ccttcaatca gtttcgtctg taccagatgc
    12061 gcttcattca atgccatccg tgactgatcc atcatcgctt ttgctgcggc aaaatcgccc
    12121 tgcttcgcct gtttcagtgc cgcatacgcc aggctacgcg cttgtccgga gttgatgata
    12181 agccccatca ccacttcttc cagctcttca gcttccgttt gcgtatcggg aatattatcg
    12241 agatccatca tacatcgttc ctctctattc ttaccggcac gattacccgt accggcatca
    12301 attaaaattt cagggcgttg gcgatatctt cttcgctctc ttctttatca atcgcgtttt
    12361 gtgctttgtt agcgaccaca acaaagggca gataaattaa cgttgcgata ccaagattga
    12421 agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg
    12481 tgggcatggt ccacggtgca atattggtca ccggtggaat aatgcccatg tagtacgctg
    12541 ccagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt
    12601 tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg ccggacggca
    12661 gcgccagttt tgccacctga cgataatcag cacggcgaga agcgataaag atagccagga
    12721 tcaggcctaa agtcgcacca ctgccaccaa ggaaaataaa ggagtccagc atcggtttgg
    12781 cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat
    12841 aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg
    12901 ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaaaatcaca taggcccagc
    12961 ctaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg atctgatgga
    13021 agtttgtgcc ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa
    13081 aacctggaat taatgctgag aaagagcgcg aaaccgatgt cggaacgcta tcaggtaact
    13141 taatgaccca gttacgatga acaataaagg taaacatttc tgccaccacc aggccaataa
    13201 taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca taggcctcac
    13261 cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaacccg gccgccagcg
    13321 catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca
    13381 ttatccctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca ttaagtgttt
    13441 caatggttga ggcatccagg cgaataccta aggaataaaa aaacgacccc tccccaaaac
    13501 ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc
    13561 gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag
    13621 ggaggagtac cttttcaagc gatgcaataa cattactcat agaaaaatac cctttaaaac
    13681 cgcaatttaa atattgcggt attgatttat gaaataactc tttgacggga aaatttaaaa
    13741 taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc acgcctaaac
    13801 catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac
    13861 gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca tcggcattct
    13921 gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga acttcatatt
    13981 tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc gcagaacaaa
    14041 acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc tgtcagccag
    14101 acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac caacatgact
    14161 ttttttgtgg ctgaaaggag tatacggtat aggcctgagc aatagtattt ctttgacctt
    14221 agaaattgtc tctccttgac ctgtggttat gaccctcttc acatttcggg caaataattc
    14281 gcgaagataa ttaagaacac aggccaaaaa aaaaccggcg caatggccgg tttccgttgt
    14341 tactcaagct ttcaggcgaa gtgattactt cgtagcctgt ggatcagtgt cgtattcagc
    14401 acaggtctga taaccggagt tgatgacatg tccggtatca tctaacgcga caaagtaggt
    14461 ttctgcttta ccatcacgtt gacccaagat gtaggtctgg caagtaccac gagcatggat
    14521 catgctcact tcagacgaag gtttacccgc aatttgtgca acctgcgccc ggctcatgcc
    14581 ttttttgacg tctttcacca caggctgtac aaactggtct ttggtacgat cataagccgt
    14641 acaacccgcc agcatggtta ataccgccgc tgcactcaga attcctgcca tattcttgtt
    14701 catattccgt cctcttgttt atcagcgtgt tagataagcc tggaatacat tgggcgcttt
    14761 ttcaagcccg tgaacgaaac ggctcctctt tcagaggatt cctgtatgac gttttaacca
    14821 ccattcagcc cgctgtcgct tgtcgtttca gtagcaacgg gttagcttta aggaagtttt
    14881 gtcttttctg tctggagggg ttcaatgaca ttgcaacaac aaataataaa ggcgctgggc
    14941 gcaaaaccgc agattaatgc tgaagaggaa attcgtcgta gtatcgattt tctgaaaagc
    15001 tacctgcaaa cttatccgtt cattaaatca ctggtgctcg ggatcagcgg cggtcaggac
    15061 tccacgcttg ccggtaagct gtgccagatg gcgattaatg agctgcgcca ggaaaccggc
    15121 aacgaatcac tgcaatttat tgccgtacgc ctgccctatg gtgttcaggc cgacgaacaa
    15181 gactgccagg atgccatcgc ctttattcaa ccggatcgcg tattaaccgt taatatcaag
    15241 ggcgcggtac tcgccagcga gcaggcattg cgggaagcag gcattgaact gagcgatttt
    15301 gtccgtggca atgaaaaagc gcgtgagcgg atgaaagcac aatatagcat tgcgggtatg
    15361 accagcggtg tcgtggtggg caccgatcat gcagcagaag ctattaccgg attcttcact
    15421 aaatatggtg acggcggtac ggacattaat ccgctgtatc gtctcaacaa acgtcagggt
    15481 aaacagttac tggcggcatt aggttgcccg gaacaccttt ataagaaagc gccaacggcc
    15541 gatcttgaag atgatcgccc ttcactgcca gatgaagtgg cactcggcgt gacctatgac
    15601 aatatcgacg actatctgga agggaaaaac gtgcctgaac aggtcgccag aacaatagag
    15661 aactggtatc tgaaaaccga acataaacgc cgtccgccaa ttaccgtttt cgatgatttc
    15721 tggaagaagt aataatttgc acatattggt ttgtgcgaaa aagagtactt tgttcacgcc
    15781 ggatgcggcg tgaacgcctt attcgcccca caaaactatg caaattcaat atattgcata
    15841 agtcgaatag gcctgacagg cgtagcacgt cagacggtgt aacctttgtc atcaacccgc
    15901 ctctttttta atcgctcccc gcctgttaca ctggatagat aaccagcatt cggagtcaac
    15961 agtggtaagg cgtttaactt ctccgcggct cgaatttgaa gctgcggcaa tttatgaata
    16021 tcccgaacat ttacgttcat tccttaatga cttacccacc cgaccagggg tgtatctgtt
    16081 tcatggcgaa agtgacacca tgccgctcta tatcggcaaa agcattaaca tccgcagtcg
    16141 ggtgctttct catttacgca cccctgatga agccgccatg ctacggcaat cccgacgcat
    16201 cagctggata tgtaccgccg gtgaaatcgg cgctctgctc cttgaagcgc gattaatcaa
    16261 agagcagcag ccgctgttta ataaacgctt gcgccgcaat cgccagctct gcgccctgca
    16321 attaaatgaa aagcgcgtcg atgtggtgta tgccaaagag gtggattttt cacgagcccc
    16381 caacctgttt ggcctgtttg ccaataggcg cgcagctttg caagcattgc agaccatcgc
    16441 tgatgaacaa aaactttgtt atggcctgtt gggactggag ccgttaagtc gcggtcgtgc
    16501 atgttttcgt tcagcgctaa aacgttgcgc cggagcatgc tgcggtaaag agagccatga
    16561 cgatcatgcg ctacgtttgc gccagtctct ggaacgttta cgagtcgttt gttggccgtg
    16621 gaaaggtgcg gtagcgctga aagaacagca cccggaaatg actcaatatc atattattca
    16681 aaactggctg tggctggggg cggttaattc gctgaaagaa gcgacaacgt taattcgggc
    16741 acccgccggg tttgatcacg acggttataa aattctttgt aagccgctgc tttccggtaa
    16801 ctatgaaatt actgaacttg atccggtgaa tgaccagcaa gccagttgat ttcactgaac
    16861 aacagtttac cttgcggttg caaaagccgc agggtgtgcc tgccatcgaa ccagcacgcg
    16921 ccctggtcgg tagtgagcag tttgtcgccc aattgccacg ccccgtttat tacaaacacc
    16981 accccgccgc gtgaaccaaa ggtagtaaac gtacgttcgg caattctgac tttcgcctta
    17041 cagacatcca ggcgtgtcat tatgttgaaa tccatcgaca tctgccctgc cgtcagtttc
    17101 gcttttacca ccaggtccgc tgcaaaggaa aaaggttgta acggttttaa ggtatggtta
    17161 aagcggtctg cgctttcaag gaacatctcg ccgccttcca gcaacgtcac tatcctttcc
    17221 ataccgggaa acaaagaaaa ctccccattc gccgcgatgg atgtaatgct ggcacgccag
    17281 taaaaatcac gttttgccgg tgggaaagtg caaatttcgc gcgtttcacc ggcagcgttt
    17341 cgccacagat tcaccgacat tttacgcata tcaaagtatt ccatacccgc tcccggcccg
    17401 ttcttttcat tgttatcgtt caggtaaccg tggatggtta tcggcaactc gggtgattta
    17461 cgttagtggc gatcaggctt tttttgcatc tgtttgagaa gagaaaagaa aaccgccgat
    17521 cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttacg ttttttggct
    17581 gaaagattat tcagcagttg caggcatttt accttttgcc gctggacgtt ctgtcagacg
    17641 cttctcaaaa ttagcattaa attgcttttt ctgttccggc gtcaggatgt tgtaaatctt
    17701 gttctgggtt tccatgtgcg ccagcatgtt agctttgcgc tgttcttcca tttttgcgat
    17761 ctgcgcttca gcttttgctt tatcgaaggt atcgctggca atgatgtcat gcattgcgcg
    17821 gcgttcttcc agcggcggac gtttcatctg gtcacgctgg cctttcatga tttcgcggat
    17881 ctgctgtttc tgcgcgtcgg tcaggttcag gtctttgaac atcatgtcct catgcggacc
    17941 gaacttgcct ttatggtgca tcatcggctt cgcgtcagcc ggtgctgcgg tagtggtgtc
    18001 tgcggcatgg gccaggttag ccgcgccaag agccagggta gaggcaacaa acagtgcagt
    18061 taatttacgc atattctata tccttccttt cagttattta ttacggcttt cttaagtagc
    18121 gtgccgtgtt gacgagatta actttactga ctttagcgtc aattaatcag agcaacggta
    18181 aaacaatgaa agtgtaaaaa acacttttgc gccaattatg gagaaaaaaa gaaaatttga
    18241 tggagagtga tgagagaata ttacaacacg atgattttgc agagattatg aagaactata
    18301 ccgga
//
LOCUS       DADTFG010000097        18021 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.097, whole genome shotgun sequence.
ACCESSION   DADTFG010000097 DADTFG010000000
VERSION     DADTFG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.097"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(170..916)
                     /gene="cutC"
                     /locus_tag="J1138_003100"
     CDS             complement(170..916)
                     /gene="cutC"
                     /locus_tag="J1138_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBA8454867.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DVHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(930..1496)
                     /locus_tag="J1138_003101"
     CDS             complement(930..1496)
                     /locus_tag="J1138_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA8454868.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLSGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1712..3445
                     /gene="argS"
                     /locus_tag="J1138_003102"
     CDS             1712..3445
                     /gene="argS"
                     /locus_tag="J1138_003102"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBA8454869.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3498..3890)
                     /locus_tag="J1138_003103"
     CDS             complement(3498..3890)
                     /locus_tag="J1138_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBA8454870.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(3887..5968)
                     /gene="flhA"
                     /locus_tag="J1138_003104"
     CDS             complement(3887..5968)
                     /gene="flhA"
                     /locus_tag="J1138_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBA8454871.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDETKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGKL"
     gene            complement(5961..7109)
                     /gene="flhB"
                     /locus_tag="J1138_003105"
     CDS             complement(5961..7109)
                     /gene="flhB"
                     /locus_tag="J1138_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBA8454872.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7318..7962)
                     /gene="cheZ"
                     /locus_tag="J1138_003106"
     CDS             complement(7318..7962)
                     /gene="cheZ"
                     /locus_tag="J1138_003106"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBA8454873.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7973..8362)
                     /gene="cheY"
                     /locus_tag="J1138_003107"
     CDS             complement(7973..8362)
                     /gene="cheY"
                     /locus_tag="J1138_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HBA8454874.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGLDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8377..9426)
                     /locus_tag="J1138_003108"
     CDS             complement(8377..9426)
                     /locus_tag="J1138_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HBA8454875.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAI
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9429..10289)
                     /gene="cheR"
                     /locus_tag="J1138_003109"
     CDS             complement(9429..10289)
                     /gene="cheR"
                     /locus_tag="J1138_003109"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBA8454876.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10308..10534)
                     /locus_tag="J1138_003110"
                     /pseudo
     CDS             complement(10308..10534)
                     /locus_tag="J1138_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10580..12241)
                     /gene="tar"
                     /locus_tag="J1138_003111"
     CDS             complement(10580..12241)
                     /gene="tar"
                     /locus_tag="J1138_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBA8454877.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(12386..12889)
                     /gene="cheW"
                     /locus_tag="J1138_003112"
     CDS             complement(12386..12889)
                     /gene="cheW"
                     /locus_tag="J1138_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBA8454878.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(12910..14874)
                     /gene="cheA"
                     /locus_tag="J1138_003113"
     CDS             complement(12910..14874)
                     /gene="cheA"
                     /locus_tag="J1138_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBA8454879.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNTIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFNYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPDDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(14879..15805)
                     /gene="motB"
                     /locus_tag="J1138_003114"
     CDS             complement(14879..15805)
                     /gene="motB"
                     /locus_tag="J1138_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBA8454880.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(15802..16689)
                     /gene="motA"
                     /locus_tag="J1138_003115"
     CDS             complement(15802..16689)
                     /gene="motA"
                     /locus_tag="J1138_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBA8454881.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(16816..17394)
                     /gene="flhC"
                     /locus_tag="J1138_003116"
     CDS             complement(16816..17394)
                     /gene="flhC"
                     /locus_tag="J1138_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBA8454882.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(17397..17747)
                     /gene="flhD"
                     /locus_tag="J1138_003117"
     CDS             complement(17397..17747)
                     /gene="flhD"
                     /locus_tag="J1138_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBA8454883.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4314 a         5004 c         4428 g         4275 t
ORIGIN      
        1 tatataaaat atttttagca caacgctatg cgcactgtca cccgctcaac agatcacggc
       61 aatcgttcgt tttttatact gctcagggat aaaaaaaggc ccctgttgaa attgcagggg
      121 cctggtacga gcaagcatca tattgggcga catgatgcaa cggtaaaaat catttggcct
      181 gatggcgttc aatgattcct ttcatttcag caaccgccgc cccgtctacg acataacgcg
      241 aatactcgtc cgcgtgtaca tctgatgaca tggacaatcc ttgattacga taacgcatcg
      301 gtgaggcttg ccacgctccc gcggagctat ggacttccag cactccggca tcgaggaagt
      361 ggtgcaggtt ttctgcacgg actcctgctc cggccataat gattggagca tcacgatggg
      421 caataagttc cataattttt gataaacctt gcagtgcgtc tgatttttgc cctgatgtca
      481 gtacccgggc aatgcccaat tccgtaagat tattgagtgt atttaaaggg ttagcgcaca
      541 tatcgaaggc gcgatgaaaa gtcactgcca gcggaccggc agcagccatt attttttcca
      601 ttcgtggcat atcgacattc ccgtcaacat cgagaacgcc cgtcaccagt ccaggaaaac
      661 ctaattcgcg gactgtgcgc acatcctcaa gaatggcggc aaattcaccg tcgctgtagc
      721 aaaaatcacc accgcgtggg cgaattatcg gatgcacagg gatcgtcacc cgctggcgca
      781 cggatttcag tacacccaac gacggcgtta agcccccctc ttttggagct gcgcataatt
      841 caactctgtc agcgccgttt tgctgcgccg ttagcgcgca ttccatgctg taacagcaaa
      901 tttccagtaa tgccattttt gctccttagt tacgccgact gctcgctggc aacgatctct
      961 tcaatggacc acggatgaaa cttaatggtt gttttaccat cggtgaccgc cagcgtcgga
     1021 ttcggtaatc gttcatgctc accttttggc gaactggttt taactgagat ccccggcagg
     1081 ctgagtcctt catcagaaag caatgccagc gcacgggcat tcagcgtttc tggatcaccc
     1141 gacagaacga tttcaatatg ttcccaacct tcgtgcgggt aacgtttttc cccgggccac
     1201 ggtagctcca caatggaaaa ctgccagtgc gcaacctgta ccggttcatg caatttaaac
     1261 agacaaatcg gtctgccatt gatcatattt tctgacaaaa gctcgccgca ctgttcaaac
     1321 ccgcgacgcc agcgttcagc ggtgacgttt tgatggcaac gcaaggaaat gtgatcggca
     1381 gtcagcggag tgatatccaa ccccagacgg cgggaaagtt catctaatgc gtgggtaaat
     1441 cgcggtaaat ccgatgcaat atcctgcagc tcgtcgatag attgccagtt cgccataatc
     1501 actcttcgtc tttcattaaa agcgctaatt taccctgttg ccctgcgcca accaaccgct
     1561 gatttcacgc cgcttctgat gcaatggtga aaacggcaat acgccacgcg cacgttgctg
     1621 acgaaaacag ccatttgcag tatactcccg ccctaatttc tttaactggt gcgggcaatt
     1681 tttgctcgct tcatcaatgt aaggtattcc ggtgaatatt caggctcttc tctcagaaaa
     1741 agtccgtcag gccatgattg cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca
     1801 gtcagcaaaa gttcagttcg gcgactatca ggctaacggc atgatggcag ttgctaaaaa
     1861 actgggtatg gcaccgcgac aattagcaga gcaggtgctg actcatctgg atcttaacgg
     1921 tatcgccagc aaagttgaga tcgccggtcc aggctttatc aacattttcc ttgatccggc
     1981 attcctggct gaacatgttc agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc
     2041 agaaaaacag accattgtgg ttgactactc tgcgccaaac gtggcgaaag agatgcatgt
     2101 cggtcacctg cgctctacca ttattggtga cgcagcagtg cgtactctgg agttcctcgg
     2161 tcacaaagtg attcgcgcaa accacgtcgg cgactggggc actcagttcg gtatgctgat
     2221 tgcatggctg gaaaagcagc agcaggaaaa cgccggtgaa atggagctgg ctgaccttga
     2281 aggtttctac cgcgatgcga aaaagcatta cgacgaggat gaagagtttg ccgagcgtgc
     2341 acgtaactac gtggtaaaac tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgtaa
     2401 actggtcgac atcaccatga cgcagaacca gatcacctac gatcgtctga acgtgacgtt
     2461 gacccgcgat gacgtcatgg gtgaaagtct ctacaaccca atgctgccgg gtatcgtggc
     2521 ggacctgaaa gccaaaggtc tggcagtaga aagtgaaggc gcgaccgttg tattccttga
     2581 tgagttcaaa aacaaggaag gcgaaccgat gggcgttatc attcagaaga aagatggcgg
     2641 ctatctctac accaccactg atatcgcctg cgcgaaatat cgttatgaaa cactgcatgc
     2701 cgatcgcgtg ctgtattaca tcgattcccg tcagcatcaa cacctgatgc aggcatgggc
     2761 gatcgtccgt aaagcgggct atgtaccgga atccgtacca ctggaacacc acatgttcgg
     2821 tatgatgctg ggcaaagatg gtaaaccgtt caaaacccgc gctggcggta cggtgaaact
     2881 ggccgatctg ctggatgaag ccctggagcg tgcacgtcgt ctggtggcag aaaagaaccc
     2941 ggatatgcca gccgacgagc tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa
     3001 atatgcggat ctctccaaaa accgcaccac ggactacatc ttcgactggg acaacatgct
     3061 ggcgtttgag ggtaataccg cgccatacat gcagtatgct tacacgcgtg tattgtccgt
     3121 gttccgtaaa gcagaaatta acgaagagca actggctgca gctccggtaa tcatccgtga
     3181 agatcgtgaa gcgcaactgg cagctcgcct gctgcagttt gaagaaaccc tcactgtggt
     3241 tgcccgtgaa ggcacgccgc atgtgatgtg tgcttacctg tacgatctgg ccggtctgtt
     3301 ctctggcttc tacgagcact gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag
     3361 ccgtctgaaa ctggcacaac tgacggcgaa gacgctgaag ctgggtctgg atacgctggg
     3421 tattgagaca gtagagcgta tgtaatcgcg ttatacggca acagccgatg tcattgcaga
     3481 catcggcttt tttatcatta acgataattt acaatcactt cattccgttg aatctttagc
     3541 ggcggaatta accgcccacc gccaggcact tcccagataa agcgcaacgg ttctgccgcc
     3601 ggtataccgg aaaaggccac ggtggttcca ctctgcccct ctaattcgac acaacgagat
     3661 tgtgaacaca agcgaacccg cagtcctgca ggtgtcggac cgataagctg ataacgccac
     3721 gccaccagcg tcatcaatcc tgaagcaggt tgtcgcgtag aaagaggcgc tgacgacatc
     3781 gattcaccgc gatgatttag cgtaacgccc acgctactcg cctgccacat cccctccccg
     3841 gcggcttgca ccagcagcgg aaacaataat attgctaata aggctctcat aatttgccgc
     3901 caatcgtcgc cgtcatacgg atatgccggt tatctgatag ttccagattc gacagcacca
     3961 ctaactgcgg caagctgcgg cgcaggaagc gagacaataa tggtcgcagc gcgtggttca
     4021 ccaacaatac tggcggcgca cccaacatct cctgacggga tagcgcttcc tgagtttgcg
     4081 ccagtaaacg atccgccagc cctggctcca gtcccccccc gccctgcaac gcctgtagca
     4141 acaaacgttc cagcggtgta tcgaggccaa taacatggac ttcatctttg ccaggaaacc
     4201 actgctgggt aatcgcccgt cccaacgcta cgcgcacgac ggcggttaat tcatgcggat
     4261 cgctttggat gggcgcatgt tccgccagcg tttcgagaat ggtgcgcata tcgcgaatcg
     4321 gcactttttc atcgagcaga ttttgcagca ctttatgcag tgtggtaagc gtgacgacgc
     4381 caggaacgag atcttccgtc agctttggca tctcctgggc gacgcgatcc agcagctgtt
     4441 gcgcctcctg acgaccaaac agctctgcgg catgctggct aatgaggtgg ttaagatgcg
     4501 ttgccaccac cgtgctggcc tcaaccacgg tgtacccctg aatctgcgcc tgctctttaa
     4561 gcgcactttc aatccagata gcattcaggc caaatgccgg atcgacggtc gcctcaccag
     4621 gtaacgtccc ggcagcggtt ccagggttaa tcgccaacca acgccccgga taagcatcac
     4681 cactgcctat ctccacgcct ttcatcaaaa tgcgatagcg ggcaggttgc agatccatat
     4741 tgtcgcgaat gtgtaccact ggcggcagaa accccatctc ctgggcaaat ttcttgcgga
     4801 tactgcgtat acggcccaac aactcaccat cctgctggaa atcgaccatc gggatcagtc
     4861 gataacccac ttccattccc agagaatctt ccagttgtac atcgttccac gtcgcttcga
     4921 caacggtatt attctctgcc atttttaccg gttttggttc agcaggcgct ttttgttcac
     4981 gtccgcggat ccaccaggcc agccccagca atccggcagt gaacagcaaa aataccaggt
     5041 tcggcattcc aggcaccagg ccgagtaaac cgagcacggc ggcgcttaat aacataacgc
     5101 tcggattact gaaaagctga ttcaccatct gctcgccaac atcctgatcg gtgctgacac
     5161 gtgtaacgat gacgcccgcg gcggtagaaa tcaccagtgc cgggatttgt gccaccagac
     5221 cgtcgccaat ggtcaatagc gtataacttt ccgccgcgtg ccccatgctc atgccatgtt
     5281 gcagcacacc gaccagcaat ccgccgacaa cgttaatgac catgatgagg atcccggcga
     5341 tggcatcgcc gcgaacaaac ttacttgccc cgtccatcga gccgtaaaaa tcggcttcct
     5401 gagtcacttc ggagcggcgt tttttcgttt catcttcacc aatcaatccg gcgttaaggt
     5461 cggcgtcaat cgccatctgc ttacccggca taccatcgag aacaaagcgc gcgcccactt
     5521 ccgcaatacg cccggcacct ttggtgataa ccataaagtt gatgatcacg agaatgacga
     5581 acaccacgat accgatagcg aaattgccac caacgaggaa gtgaccgaac gcttcgacca
     5641 cctttccagc cgccgccgcg ccggtatgcc cttccatcaa aatgatacgg gttgaagcca
     5701 cgttaagtgc cagacgcaac agcgtggtaa acaacaggat ggtcggaaac gcagcaaact
     5761 cgagcgtgcg ctgggtaaac atcgccacca gcaacaccat gatcgacaag gcaatattga
     5821 aggtaaacag caggtcgagt atgaatgcgg gcagtggcag taccatcatc gacaagatca
     5881 acaggatcaa aatcggtccg gcaaggatct gccattgtgt cgatttcagg tttgcaggca
     5941 ggcgcagcat cgcggccaga ttactcatgg gtcggtttct cgttaataaa atccagggct
     6001 tccggcaccg gaagatgagt aggttgtaca gggcgctgtc caccagccag acgccagcgt
     6061 ttcagttgcc agacccaggc cagcacttcc gccaccgcgg cgtacagttg acccgggatt
     6121 tgttgaccga tctccgcatg tcgatacagc gcacgcgcca gcggcggcgc ttcaagcgtc
     6181 gggacgttat tttcagcgcc aatttcacga atgcgcagcg cgaccagccc tgcaccttta
     6241 gcgaccactt tcggtgcgct cattttgttt tcgtcatact gcaacgctac cgaatagtgg
     6301 gtcgggttat tgacaatgac atccgctttc ggcacatcgg ccatcatccg acgccgtgcg
     6361 gccgctcgct gcatctgacg gatccgccct ttaacatggg ggtcgccttc gctttgtttg
     6421 aactcatcac gaatatcctg ccgtgacatt cgcagctttt tcaggtggct gaagatttgg
     6481 aaaaagacgt caaatcccac cattggaatg acaccaagca ccaccagcag cgcgcatagc
     6541 cctaccagat ccatcgcatt acccatggcg gtaatcggag actcggccat taagcgcatc
     6601 atctgcggcc agtgatgcca gagaaaaaac cccgtcacgc tgccaaccag gatggttttc
     6661 aaaattgcct taagcaactc cgcgccagtc tgtgccgaga acatccgttt aatgcccggt
     6721 agcgggttga gtttggaaaa cttcggctgc aaggattttc cgctaaatac cagccccccc
     6781 agcataaccg gagaaataat cgccaccagc accacgccgc taatcagcgg tagcagcgcc
     6841 agcatggctt ctctgatcag cagaataatc tgcccgagga tcagattcgg gtcattgata
     6901 atactgtgat caaaatgcag ccctgcggag agcatgcccg acaatcgacg ggccagcgac
     6961 acaccgccaa accagataac actaacgccc accagcaaaa tcagcagtga agtcagttcg
     7021 cgggaacgcg ggatttgccc ctcttcccgc gctttttcta gtcggtgagg tgtgggggct
     7081 tctgttttgt cgtcgctctc gtcagacacg tcgccaatcc tggaaagagt attaagcgta
     7141 aatgatgcca gagcgcaaag tgttcaatgg tttgagtaag gggcaaaaca ggcgggattt
     7201 agggcttttg ccacagcaca tcaggcatgg tgacatgttt gtcgggtacg gcgtaaacgc
     7261 cttatccaac cttgaagatc tgatgtgacg cggccacgtc acatcaggca atacaaatca
     7321 aaatccaaga ctatccaaca aatcgtccac ctgatcctga ctggctacca caccggcttt
     7381 gctggtatcg acctgaggtc cattaagcaa actctggttt tcacgttttg gacgcgactc
     7441 ctgttccggg atgttttcca acagcaccat cagcaactga cgttcgatct cctgaatgac
     7501 atccatcatc cgcttaatga cctgtccggt gagatcctga aaatcctgcg ccatcatgat
     7561 ttccagcagt tgcgcgttag taaagctggt atgtgcgggg acatctgcca gaaattgccg
     7621 cgtgtctgtt accagttcac gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc
     7681 ccaacgttgg gttaacgctt ttgctgattt ctccatttga tcctgatgcg gttgtgacgc
     7741 ctcaacactg ttcagcgccc gctccgcagc ctgggcggtc atctgcacaa catagtacaa
     7801 acgatcgcgc gcatcgggga tagcttccgc ggcttcggca atggcctgat ccagccccag
     7861 ttcccgcaaa ctgtcgcgca gcatacgcgt caggctgccg atgcgcgcaa tgatatcgcc
     7921 agctgaatgc tcgtcagcag gtttgattga tggttgcatc atagtcgcat cctcacatgc
     7981 ccagtttctc aaagattttg ttgagttttt cctccagcgt cgcggcggta aatggtttca
     8041 ccacatagcc actggccccc gcttgcgccg cagcaatgat gttctctttc ttcgcttctg
     8101 cagtcaccat taacactggc aatgccgaca tcgcgccatc cgcacgaatc gttttcagca
     8161 actccaggcc atccatattg ggcatgttcc agtcggagat aacaaatcca taaccgcctg
     8221 cctgcaactt attgagagcg tcgaggccat cttccgcttc ctcaacatta ttgaatccca
     8281 gctctttcag caggttacgc actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa
     8341 atttaagttc tttatccgcc atttcacact cctgatttaa atacgtatcg cctgtccggc
     8401 actaattttt gccagcattt gctgacttac ctggctaaga tcgaccactt cgcagacacc
     8461 acccatattg atggcctcgc gcggcatgcc gaacaccacg caacttgctt cgttttgcgc
     8521 aagggtccat gcccccgcct gacgcatcgc caacattccc gccgcgccgt cgttgcccat
     8581 accggttagg attaccccaa tcgcattacg ccccgcctgt ttggccacag aatggaacaa
     8641 cacatctacc gatggccgat ggcgattaac cgccgggcca tcgtgaattt tgatttgata
     8701 atttgcgcca ctacgcgcca gctccatatg ccgatcgccc ggcgcgatat aggcatgtcc
     8761 cggcaagacg cgctctccgt cttcggcttc tttaaccccg atctggcaaa gcttattaag
     8821 tctgtcggca aaagagcggg tgaaaccggg cggcatatgc tgggtaatta acagtgccgg
     8881 actggaaagc ggcaacggtt gcagtacgtg acgaattgcc tcagttccac ccgttgaagc
     8941 accaatcgca atcagttttt cagaactcaa caacggccct gccttcagcg ttgtcggtac
     9001 cgacaatggc ttatgtgctg caaggctcgc ctttgctgcc gtacgcacct tttcagcaat
     9061 catttcgcta tatgccagca ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc
     9121 tatcgccccc agctccagcg cgcgcagcgt aacttctgac cctttgccgg tcagggaaga
     9181 aaccatcaca acgggcattg gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc
     9241 catccgcggc atttcaacat ccagcgtcaa tacatcggga ttgaatttct taatcaaatc
     9301 acgcgcaacc agcggatcag gggcggtcgc caccatttcc atgtcgctat ggctgttgat
     9361 gatttctgtc atgatctggc gcatcagtgc cgaatcatcg acagataaca ccctgatttt
     9421 gctcatcgtt aatccttact tagcgcatac accgtctgac cacgcagcgt gaagcggcgc
     9481 tcaaggtggc taaagttttc agagtgaccc gcaaacagca atccgtcggg tttaaggagc
     9541 gaaacaaagc ggcgcaaaat ctcctgctgg gtagtttgat cgaagtagat catgacgtta
     9601 cgacagaaga tcgcatcaaa cggccccggc acggtgtact gtttcgccag caaattcagc
     9661 ggggcaaaat caacatagtt cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt
     9721 cccgtccctc gcatgaaata ccgttgcagt tgctgcggcg tcaggttttt caactcttca
     9781 tggcgataaa taccgcttct ggctttttcc agcacttcgg tgtcgatatc gctggcaaac
     9841 actttccagc gtccgggcgc ggtgcccaat gtatcagcca gcgtcatcgc tatgctgtac
     9901 ggctcttcgc cggttgaagc cgccgcgctc catacgcgat actcgccaga acggcgacgt
     9961 gcgtgatccg cgagcagagg aaaatggtgt gcctcacgga aaaatgccgt cagattcgtg
    10021 gtcagcgaat tgataaacgc ctgccactca ccgctgtgct gattagattc cagcaagttc
    10081 aggtaatgac cgaaatccgc cagtcccagc gcacgcaaac gacgaaccag tcggttgtaa
    10141 accatgtcgc gtttatggtc agccagaacg atcccggctc gttgatagat caattgactt
    10201 atccgccgaa aatgcgcgtc ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac
    10261 gtttgcccac agggcagaga tgaagtcata gcgccttctc aatcacttca ggataccact
    10321 ggcgcaattt gtaactgcgc cgactcatgt cgtgctactt catgttcttc aagggtaaat
    10381 accgccacac gcgacgaaag atggtcggcc tgattcagtc gttgcaaatc gtcgcgaaat
    10441 gcccaaaaga catgccgtta ctgccaatct gcaagatccc gcagagaatc aaaattaaaa
    10501 acagcgtggt cgaaattcga atacgattaa acatcaacgc tccccatcag gcggcaatga
    10561 ccgctttagt aaatactcgt caaaatgttt cccagtttgg atcttgttca gcaattcgca
    10621 gtcgtggctg tgccggtggt tgctcactgg caggacggga tggtgtttgc ggtttattgg
    10681 tgagtgggct ggctgccaga cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt
    10741 cttccagcgc agctgcggcg gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga
    10801 cgcgatccat ttccgaaacc gccaatgcga cttgatcgat gccacgactc tgttcatccg
    10861 atgctgatgc aatctcgccc ataatgtcag tcacgcgagt gacagcattg acgatattgt
    10921 tcattgtttc cccagcgctt tcgaccagca ccgaaccggt atcaacgcgc gagacggagt
    10981 cttcaatgag ggctttgatc tcttttgccg cctgtgcact gcgactggca agattacgca
    11041 cttcacccgc caccacggca aaaccacggc cctgttcacc cgctcgcgcg gcttcaacag
    11101 cggcattcag cgcgaggata ttagtctgga aggcaatacc atcaataacg ctgataatgt
    11161 cggcaatttt cttcgaacta tcggcgatct catgcatcgt tttcactacg ccatccacca
    11221 ctttgccgcc gtgctgggcg gtgtcggagg cactttgcgc cagttgcgag gcctggcggg
    11281 cgttatcggc gttttgcttc actgtcgcgg tgagctgctc catgctggcg gcagtttctt
    11341 caagcgcgga tgcctgctgt tcagtacggg aggaaagatc ggtgttgccc gccgcaattt
    11401 cacgggtacc ggcatagatg gcatctgaac cttcgcgcac atgagtgacc gtgtcagtca
    11461 aagagcgttg catatgtgaa acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa
    11521 tggtcagggt attcgccagg ttaccaccgg caatttcgcg aatatgcgca ataatttttg
    11581 ccagcggtgt aagcaacata cggcgaatac cgtaccacgc caccagcaga atcaatacca
    11641 ccaccagcgc gataaccgcc agttgccact gggcaaatcg gtaatcatct gcgttgtcag
    11701 tgacgatatc gcgatacagt ttttcactgc tgagggcgta ctgagcaaac gcttcgccca
    11761 ttgcattttg cattccctgg gttggctgag cgaaataagc tccagtattg ccataatcaa
    11821 gataatcaat cagttcagtt aacgctgtgt gatagttttt atatttttca tcaatattac
    11881 gactggtagc gaccatttca ggtaacggtg ccatgctttt gaatttttta taatgagtcg
    11941 cggcctgggc caatgttttc ctggcgctat cgagcaattc aactttggca ttactttgct
    12001 gattggatga atccatcatc atccgcaccg ctgaacgact caggttaatg cgcgtttgca
    12061 gcattaaatc ccaggttgac gtcagctcgc cctgctgttc ccgtaattga ttggaaacca
    12121 caaagctctt ctggctatgg tgaagggaag aaaaaaacag actgccggaa ataagctgta
    12181 acagtgcgaa tacccccagc accattacca acagcgtgac tacgcggata cggttaatca
    12241 taaggcacct tcctgaaaac aagttgatct cgttatcggc aaggaggggg gaaactttat
    12301 tgcagatgcc acctaccgcg aaattgaaat aaaaaacccg atgcgcagat catcgggttc
    12361 attgcaattg aggaaatcag gagaattacg ccacttctga cgccgcgcta tctaacagcg
    12421 ccatctcttc gctgttcagc agtttttcga tgttcaccag aatcaacatc cggtcgccca
    12481 gtgcacccag tccagtgaga tattcggttg aaagcgtcac ggcaaattcc ggtgccggac
    12541 gaatttgctc cgccgtcaat gaaagcacgt ctgagacgcc gtcaaccacg atgccgacca
    12601 cccgctgtcc gagattcagg acgataacta ccgtgttgtc gttatagtcc acatccacct
    12661 ggctgaactt aattcgtaag tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc
    12721 ctttgataaa cgctggcgtg ttcgcaatcc gtgtcacctg atcgtagcca cggatctcct
    12781 gcactttcag aatatcaata ccgtactctt catcaccaag ggtaaatacc agaaattcct
    12841 ggcctgacgg ctcgctggcc agctttgtta cattcgtcat accggtcata ttgttacctt
    12901 tttactcatt caggcggcgg tgttcgccat acgttgttcg cggtttatcg cctgcaaggc
    12961 ggagacatca acaatcagtg ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc
    13021 ggggactttg cgatagttac tttcaaggtt tttaaccaca acctggtgtt gaccaattaa
    13081 ttgatccacc agcaaggcgt agcggcgacc gccactttgt aagatcacca caattccctg
    13141 ggtggcttcg gttttcgcgc ccgcgacgtt gaacactttc cacagttcga cgataggcag
    13201 atattcaccc cgcacttcca gcacccgctc gccgccagcc agtggatgca gatcggcttc
    13261 acggggttgc agtgattcca tgacagcatt cagcggcaga atgaaaactt catccgcaac
    13321 gcgtacggac atgccgtcaa gaattgccag cgtcagcggc agtaaaatgc ggatcgtagt
    13381 gccagtaccc tgcttcgact ggatctcgac atgaccgccc atctcctgga tattacgttt
    13441 aacgacgtcc atgccgacgc cacgcccgga gacgtcggtg acctgctccg ccgtggagaa
    13501 gccaggggca aatatcagca ttgcaacttc gtcgtcgctc atgttttcgc tgacggtcag
    13561 accttgcgag gccgcttttg ccagaattcg ttcacggttt agccccgcgc catcgtcggt
    13621 cacttcaatg caaatgttgc cgccctgatg ttcggcagac agaattaaat ttccgacgct
    13681 atttttacct gcggcgagcc gtttttctgg cagttcaata ccgtgatcga ggctattgcg
    13741 taccaggtgg gtcagcgggt cgataatgcg ttctatcagg ctcttgtcga gttcagtgga
    13801 actgcccacc agcgtcagtt ctacctgctt gccgagtttt cccgccagat cacgcaccag
    13861 ccggggatag cgactaaaga catattccat cggcatcatg cgaatcgaca tcactgattc
    13921 ctgcaaatca cgagcgttac gttgtaactg ccccatgctg gtaatcaaat caccatgatt
    13981 aaccgggtcc agttcactgg agcgctgggc aagcatggac tgggtgatga ccagctcgcc
    14041 gacgaggtta attaattgat caaccttttc taccgctaca cggatgctgg tggattcact
    14101 gctgcgcgtc gttttttccc gctccacgcg accggttggc gcttgttcgg ctgccagttt
    14161 aagcactggt ggggtggata tttttggcga gacgtctact gtttcaaagg taatctgatc
    14221 ggcttcaatc acaaaacaga gtaccgcagt gatgtcatct tcggcgatgt cgtccggtaa
    14281 tattgccgag agggaatcag cccctttcac cacgtcagtt aacgttgtca gatgccccaa
    14341 ctcttcttcc agcaggtcga cttcgccggc cttcaggcgc gaaaggataa ttcgtcgcgg
    14401 tgactgacta cgactctgct catcttgcgg ttcacttttg gcaaccacac ttaatcgggt
    14461 cactgcggat ggcgtttcgc ctttcgcttc taatgccagt tgacgtaagg cctggcagat
    14521 ataattgaag ctggcggcat ccggctcttg cgactgttta taagcgtcga gctgttcttg
    14581 catgatgtcc ttcgtttcca aaaacagatt gataatgtcg gtgttgagtt gcatctcacc
    14641 tcgtctggct tcatcgagca ggttttccat cagatgcgtg gtttcctgca aaacgctgaa
    14701 gccaaaggtt cctgcccctc ctttgatcga gtgggcagcc cgaaagatgg tattcaattg
    14761 ttcggcatct ggcgcttccg gctgcaaaac cagcaaatgc tgctccatat cagccaacag
    14821 ttcgtccgct tcatcaaaaa atgtctgata aaaatcgctt atatccatgc tcacgctgtc
    14881 acctcggttc ggctgatggc attgtgggaa cacttacctg tggtgcaacc tcaggttttt
    14941 ccagggcgct tactggctca ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg
    15001 cttgtttgtt cagtaccagc aggctgatgc gacggttgac ggcatcatca ggtccgcgat
    15061 cgcttaagcg catcgttgcc gccatgccga cgacacgtaa cactttgccg ccattcaacc
    15121 caccaaccat cagttcgcgg cgggatgcat tggcacgatc ggcagaaagc tcccagttgc
    15181 tgtaaccttt ctcaccgctg gcgtagggaa aatcatcggt atgacccgag aggctaattc
    15241 ggttgggaat accattcaga acgggcgcga tggcgcgcag aatgtcgcgc atatagggtt
    15301 ccacatcggc actgccggtt ctaaacatcg ggcgattctg gctatcgatg atctgaatac
    15361 gtagaccttc ctggaccaga tcgattttga gatgtggacg taacgcccgc agtttcggat
    15421 cggactcaat caactggtcg agatcgccac gcaatttccg caatcgactt tgctccatgc
    15481 gttttttcag ctcttcgatg ttcggctgct tattcacttc cccctggctt tgggtgtaat
    15541 catcaccacc gccgggaatt gggctttcac tattagaaat gcgatcgccg cccgtaaccg
    15601 ctgtcgccag cggagtccgg aagtactccg caatctgaat cagctctttt ggactggaga
    15661 tagagatcag ccacatcacc agaaaaaagg ccatcattgc ggtcataaag tcggcataag
    15721 caatcttcca cgatccatgt gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa
    15781 taatcggatg cgcttgattc ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt
    15841 caccgcacgc acatgctctt ccagctcaat aaacgacgga cgttcgctgg aatagagcgt
    15901 tttgcgacca aactcaacgg cgataggcgg tgcgtaaccg ttcagatttg aaagcagagt
    15961 aactttgacg cactgcatca ttttgctggt ttcggcgctt ttctgacgta aaacactcgc
    16021 taatggggaa ataaatccgt aagccaataa aataccgagg aaagtcccca ccatcgcatg
    16081 agcgataagc gcccccagct cggcggcagg acgatcggct gaacctaacg cgtgaactac
    16141 ccccattaca gccgcaacaa taccaaacgc cggaagtgag tccccgacca gcgccagact
    16201 gttcgccggg acttctgcct cgctttcgtg cgtctcaatc tcttcatcca tcagagcttc
    16261 gatttcgaag gtgttcatgt gaccgctgat aatcaggcgc agataatcga cgataaattc
    16321 aagcatgacg ctatccgcaa ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg
    16381 attttcaata tcgcgttcca gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg
    16441 ataaagcaga gccagcagat ccatatacat tgctttggtg tatttggagc gacgaaacag
    16501 caacggcagc gccttcagcg tgcctttaat cgctttgcca ttattgccga cgataaacga
    16561 cccaatccct gcaccggcaa taatcaccag ttcagcgggt tgatagagtg ctccaaggct
    16621 tccaccggtc atcaaataac cgccgaaaac tgtaccgaga acaaccaggt aacctaataa
    16681 gataagcacg acatcatcct tccactgttg accatgacag gatgttcagt cgtcaggcgt
    16741 taacacgcga taggggcaaa aaaaagcagc ggtacgtcgt taccgctgct ggaatgtttc
    16801 gccacaccgt atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat
    16861 atcggcagga ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gctgacataa
    16921 gctgcaggca aagctgccaa caggttggtg agcgtgggta ataaaattgc cgccgcagca
    16981 gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgcaccaatg tccaggcacg
    17041 ggttaatgcc agcagcggtc cttcttctgc ttgcgggcac tgttcaaggt ataaacggta
    17101 ggctttgatc accgcatcga cgccattaca caagccagtt ttcagtaaaa actgccatgc
    17161 attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa
    17221 tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttatagagtt ttatcagacg
    17281 tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtgatcaa
    17341 ttccattgcc agctgaatat cccgcgcttc ctgaacaatg cttttttcac tcatgatcag
    17401 gcccttttct tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa gcgtgttgag
    17461 agcatgatgc cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg cgtcaactga
    17521 gtaatcgtct ggtggctgtc aaaacggaag tgacaaacca gttgattggt ttctgccagc
    17581 ttaaccattt gcggaagagt cagtgccgct aacgttgtcg ccatttcttc atttatgccg
    17641 agacgaaaca tagcggacgc tttgtcctga acaatcagac gctgtgcaag tagtaaatat
    17701 gacaagttga tgtcataaat gtgtttcagc aactcggagg tatgcattat tcccacccag
    17761 aataaccaac tttattttta tgcggtctca ccgcaccccg tgatgtcgcc gggaagcccc
    17821 ggtaaaaaat aattagcatt agaatagttg cgataagctg caataagcag acccaccttt
    17881 ttggtttaat aagtccttac aaatagaagg gggtctttac acttatctaa gatttttcct
    17941 aaatcgacgc aactgtactc gtcactccac gcacatacaa cggagggggg ctgcgatttt
    18001 caataatgtg tgatgcagat c
//
LOCUS       DADTFG010000098        17991 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.098, whole genome shotgun sequence.
ACCESSION   DADTFG010000098 DADTFG010000000
VERSION     DADTFG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.098"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            341..1240
                     /gene="yegS"
                     /locus_tag="J1138_003118"
     CDS             341..1240
                     /gene="yegS"
                     /locus_tag="J1138_003118"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBA8454884.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAVRFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEEASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1322..2101)
                     /locus_tag="J1138_003119"
     CDS             complement(1322..2101)
                     /locus_tag="J1138_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8454885.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2201..3241)
                     /gene="gatD"
                     /locus_tag="J1138_003120"
     CDS             complement(2201..3241)
                     /gene="gatD"
                     /locus_tag="J1138_003120"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBA8454886.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRDLRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3289..4644)
                     /locus_tag="J1138_003121"
     CDS             complement(3289..4644)
                     /locus_tag="J1138_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HBA8454887.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIMFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4648..4932)
                     /gene="gatB"
                     /locus_tag="J1138_003122"
     CDS             complement(4648..4932)
                     /gene="gatB"
                     /locus_tag="J1138_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBA8454888.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4963..5415)
                     /gene="gatA"
                     /locus_tag="J1138_003123"
     CDS             complement(4963..5415)
                     /gene="gatA"
                     /locus_tag="J1138_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBA8454889.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5425..6687)
                     /gene="gatZ"
                     /locus_tag="J1138_003124"
     CDS             complement(5425..6687)
                     /gene="gatZ"
                     /locus_tag="J1138_003124"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBA8454890.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSRCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6716..7570)
                     /gene="gatY"
                     /locus_tag="J1138_003125"
     CDS             complement(6716..7570)
                     /gene="gatY"
                     /locus_tag="J1138_003125"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBA8454891.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7799..8851)
                     /gene="fbaB"
                     /locus_tag="J1138_003126"
     CDS             complement(7799..8851)
                     /gene="fbaB"
                     /locus_tag="J1138_003126"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBA8454892.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFSANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9108..10385
                     /locus_tag="J1138_003127"
     CDS             9108..10385
                     /locus_tag="J1138_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8454893.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYDDISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10382..11386
                     /locus_tag="J1138_003128"
     CDS             10382..11386
                     /locus_tag="J1138_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBA8454894.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKLVAELENNGVTNGAAMRVSPLGCLLPARDIDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNTIDPALKAELDTVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11383..12348
                     /locus_tag="J1138_003129"
     CDS             11383..12348
                     /locus_tag="J1138_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA8454895.1"
                     /translation="MSGARLHTLLPELTSRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHENV"
     gene            complement(12322..13068)
                     /locus_tag="J1138_003130"
     CDS             complement(12322..13068)
                     /locus_tag="J1138_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA8454896.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRNGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13120..13938)
                     /locus_tag="J1138_003131"
     CDS             complement(13120..13938)
                     /locus_tag="J1138_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBA8454897.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFLRNWQLNRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQGFVDGIKETP"
     gene            complement(14003..14803)
                     /gene="thiD"
                     /locus_tag="J1138_003132"
     CDS             complement(14003..14803)
                     /gene="thiD"
                     /locus_tag="J1138_003132"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBA8454898.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLCSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALCPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14800..15588)
                     /gene="thiM"
                     /locus_tag="J1138_003133"
     CDS             complement(14800..15588)
                     /gene="thiM"
                     /locus_tag="J1138_003133"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBA8454899.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANVIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15623..15719)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15811..16083)
                     /gene="rcnR"
                     /locus_tag="J1138_003134"
     CDS             complement(15811..16083)
                     /gene="rcnR"
                     /locus_tag="J1138_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBA8454900.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16204..17028
                     /locus_tag="J1138_003135"
     CDS             16204..17028
                     /locus_tag="J1138_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBA8454901.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17247..17585
                     /gene="rcnB"
                     /locus_tag="J1138_003136"
     CDS             17247..17585
                     /gene="rcnB"
                     /locus_tag="J1138_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBA8454902.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17667..>17991)
                     /locus_tag="J1138_003137"
     CDS             complement(17667..>17991)
                     /locus_tag="J1138_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8454903.1"
                     /translation="GQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESNGT
                     PVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
BASE COUNT         4423 a         4624 c         4334 g         4610 t
ORIGIN      
        1 ataggattat aataaatgtt ttgtacaaca tttcctacat aagtaggaat tacagacatg
       61 gtggcccttc cgggtaaccc tatggggggc ttaaatatat tttgaatagt atatttaaga
      121 tgccactatt tagttgaata ttagatattt gctctttttt caaatttact ggtggctacc
      181 gttaatattc gctgttccca ttgcaagctc ctggtggtaa ccactgaatc ctccatactt
      241 gaactgactt tttatcctcc gactttcatc ctgttctgac tccacctttt cttttctgct
      301 ctacactatc tacagaccaa tcataaaggc acatacgatc atggcagaat ttcccgccag
      361 cttactgatt cttaatggca aaagtactga caatctaccc ttgcgcgaag caattatgct
      421 gttgcgtgag gaaggaatga cgatccatgt gcgggttacc tgggagaaag gtgatgccgt
      481 acgatttgta gaggaggccc ggaagttagg tgtcgcaaca gtgattgccg gtggtggcga
      541 tggcaccatt aatgaagttt ctacggcatt gattcaatgt gagggggatg acatacccgc
      601 gctgggaatt ttaccattag gaactgccaa tgattttgcc accagtgtag ggattcctga
      661 ggcactggat aaggcgctga aactggcaat tgccggtaac gccattgcga tagatatggc
      721 gcaggtcaac aaacaaacct gttttattaa tatggcgaca ggcggatttg ggacgcgtat
      781 taccacagaa acgccggaaa aattaaaagc cgcgctgggt ggcgtctctt acatcattca
      841 tggcttaatg cgcatggaca ctctgcaacc ggaccgttgt gaaatccgcg gtgaaaactt
      901 tcactggcaa ggtgacgccc tggtcattgg tattggtaac gggcgtcagg ccggtggcgg
      961 tcaacaattg tgcccgaacg cattaattaa cgatggcttg ctgcaactgc gcatttttac
     1021 cggcgatgaa attattccga ctctcgtatc aaccttaaaa tctgacgaag ataacccgaa
     1081 tattatcgaa gaagcttcgt cgtggtttga tatacaagcc ccacacgaaa tcacttttaa
     1141 tcttgatggc gaaccgttga gtgggcaaaa cttccatatt gaaatacttc cggcggcgtt
     1201 gcgttgtcga ttaccaccag attgtccatt gttgcgttaa tcaaactatt ctctgttgaa
     1261 ccagggataa tccgtaatgc cggatgcggt gtaaacacct tatccggcat acagaacaat
     1321 actacaccgt aattaatttt acccgtgtct tctgcaatgc cagtttatcc acctcagaca
     1381 cagcatcatc ggtaataata atgtcaattt tttcgattgg cagtacctga ttaaaaccgc
     1441 gacgattaaa tttcgatgaa tcgagaacgg caatcacttt attcgccgct gtcaccatcg
     1501 caccgctaac ggaataacct tcattaaagg tcgtaatacc attaaccgca tcaataccat
     1561 cagcaccgac aaacattaaa tcggcattaa tatcctgcag ggaacgttca gcaatagatc
     1621 catgcataga tcgcgtttta tggcggaccg tgccaccgca gacgaccaga gtaatgtctt
     1681 tattttcgga aagggcaaac gccgccggga gactgttggt aatcaccgtg atatttttgg
     1741 cggtcattaa tccttcggcg ataagcattg tggtacttcc gctgtcgaga ataacggtca
     1801 tcccttcgtg gatcattttg actgccgcct gcgctattct gtttttcggc gcgctggcga
     1861 gctgaaatcg ctctttaaac ccgacttcct gggtctcggt ttcgctatta ccggacatta
     1921 ttttcgccgc accgccatga aagcgcgtaa ccacgccttt ttgttcgaga aagcgcaaat
     1981 cggcacggat tgtcgcttcc gaggcagcaa atactcccgc cagatcctga acaagcacgg
     2041 ttccctgttc attcactaat tggatgatct tattccttcg ctcgaatgaa ttcatcgtca
     2101 tttacccata ttcaattgtg gctagtgtaa acgaaggggg accattggtg aatgatctgt
     2161 tttgcacaat accgggcctg catcacgctg gcccgcggtt tcagggaatg agcaacactt
     2221 tgcccggcat ggcattacga gcgatgtcac gcaccgcctg ggcgaagctt tcaaagcttc
     2281 cacggtgagc gattaatggc tccaggctta acttacgttc tgtcagcaac cggctcgccg
     2341 tttcccactc ctgccccggc caagggctgg agtagttcat ccaactgccg ataaccgtta
     2401 gctctttacg caatattttg ccaaacgttg cagatgttaa atgcagatcc tgatgcaacg
     2461 tgcctaccag cgccagttgg gcatgaggac cggcaatctc taccgccagt tcgacggttt
     2521 gcgggacgcc agccgtctcg aggataagct gattaaagcg cagatcgcgt aaaacgccct
     2581 gtatttgcgg cgcgctcatt tcacggctgt taaatgtttg catcgcaccg aaagattttg
     2641 ccagtgccag tttttctgag ctaatgtcga tagccgtcac actctttgct cccagcgcga
     2701 cagcgcactg aattgccagc aggccaatgg ttccggcacc aataataata acgtttttat
     2761 tctcacatcc ttgcgctaaa tggaacgcat gcaggccaac ggtaatcggt tcaataaaag
     2821 cgccatcctc aataggcata tccgcaggta gtgcaaagac attgtttcgc ttaacgacaa
     2881 tatattcggc aaatccacca tcacgccgtg agccaataaa atcatatttt gcgcactggg
     2941 aataaaaccc tttcagacac tctggacaag taaaacaggg taataacggc acacaggcaa
     3001 ccgcatcgcc ggggtgtaaa tcatcaacac cggatcccac cgcatcaata tagccactaa
     3061 attcatggcc taacgttatt ggataataat gtgcaccatt tttaaatatc ctgggtaaat
     3121 cggaaccaca taagcccgag ctggcaattt ttacccgcac ctcatcctga tgtttaattt
     3181 caggaatgac gctttctgct acgcgcacga taccatcagt atcattcacc actgatttca
     3241 taaaaactcc tgattgttaa gggggataac ctccccctca ggtaaaaatt attctgcgag
     3301 aacgactttc tcttgtttga taaagccgcg cgccctacgc caggtcatga aaataccggt
     3361 gagataaatg gcgccgataa taatgaaacc gggaatattt tgcggggaga aaacctgaat
     3421 cagtaaccag gtaatgggag aaccgccctg atccattgaa gccaccatac ccccggcttt
     3481 taacgcgccc gcattagccg ccagttgggt gtgcaaacca atagtttgcg tcgcgatcca
     3541 cagggtgatg ctcataatga tgacacccga gattaaggtg cggaacagat ttccacgatg
     3601 caccgcgacc gccatcgcca caaagaagcc gatggtggca aggtcgccaa acggcagcac
     3661 ctgattaccc ggcacacaaa cagcaattaa aatggtgagt ggaataaaaa tcaggcttgc
     3721 cgataccacc gccgtatgtc ccagcagcaa cgccggatca aggccaatca ggaactcctg
     3781 accgccgaac ttcgcctgta aacgactacg ggcctgctta gcgatgggcg ttaaaccatc
     3841 catgatgggt ttaatcaccc gtggcatcag cagcataact gccgccgttt ttaccgccag
     3901 ctgcaataca cctttgacat cgtaacccgc gaggatgccg ataatcagcc ccatcacaaa
     3961 acccacggtg accggttcgc caaaagggcc aaatttgcgc tgaatatcat cggcgctaaa
     4021 tttaattcgg ttaacgcctg ggattttctc gatgatagca tcgaccagca ccgcaatcgg
     4081 ccccatatac gccgacgtac cgtgcggaat agcaatgcct tccagctcaa agaaatttcg
     4141 ggtatcgcgg gcaaaccagt cgccgagctt ataaacaaac gccgcgtgaa ttaccacacc
     4201 cgccatccct atcatccatg aaccggttgc cagatgcagc aacgcgccgg tgaaggtcat
     4261 atgccagata ttccagatat caacatttac cacccgcgtc atacgggtca gtaacatcgc
     4321 cacgttaacc agaatcgcaa tcggaatcgc caccagcgca atttgcgacg cccaggtcat
     4381 gggtgaagag cccggccagc cgacatcgac cacatgcaga ttcaggtcga aattttccgc
     4441 catcgctttc gccgccgggc caatggaatc cagcattaag ccaatcacaa ggccaatgcc
     4501 aacaaagcca atcccgatat gcagacctgc tttaaagcaa tcgcctgcct tcatgcctaa
     4561 tattttagaa aacataatga tgacaatcgg cagcatcacc gtagggccga ggtcgagaat
     4621 ataacgcatg acttctgaaa acataggtca cccctgtaag atagtcagaa ttttattttg
     4681 taatgcttcg ataccgacac cagaaacaaa aggcatgccg tgaactaacg gaatatcgcc
     4741 aaaactacga tccaccctgg cagtggtgca tatcaaatgc acgccatcca tataggtttc
     4801 tatttcatta acccgacact ggattaattc aacaggaata ttatgactct gacacaactc
     4861 tttaatttct tccgccgcca tcgtagaggt cgcaaccgcg cctccgcaag cgacaataat
     4921 tttgcgtttc ataaatagtc ctttttattg ttacagaggg atttattcgt ctgaatctaa
     4981 aacatacttt gtgaagtatt cctttaactg ggtttcagga agagtgatta gtgtctcgac
     5041 gatatcaggc tgttgtaact tgccaaataa acagcgtaaa agtttcaatt gctgctgcgg
     5101 attttccaca atcaacgcaa taaccaacga taccgccacg tcgttatcat catccgcttg
     5161 ctgaaaatga actttatttg ttggccttaa cagataaatg gctgacgact tagcatgaat
     5221 cgcctcacaa tgcggtattg caatggcgtg ctgctcaagc attatcccgg tagggaattc
     5281 tgcttctctg gcaattaatg cctgtggcca ggtatcatga accacacctt tcgcgagcat
     5341 ctcattaccg atatgggtta aaacttcgct acgatcgaca aaagaaattc cgctacgaac
     5401 aaacaggtta gtcatatacc gtccttattc cgcacagccg tagcgatagg cgcgcaaaac
     5461 atcataaatt ttatccataa taagctgatg cggtattgct gataattccc cggactgaat
     5521 gcgttcaaat tgtttcggaa gatactgact aatcatgccc agtgggatgt ccacgccttc
     5581 cagattcacc atcatcgttt cgacgctatt tttaatccgg ctatgcggcc agtaataacg
     5641 aatacgatcc gacaggctgt aacgaatatc cagcaatgaa tcgttaaaac ccgtgcgata
     5701 atattttttc cagtattgcg gttcgtcgag catcacttct tcaatcaccg ccagacaacg
     5761 gctacgattt tctggagcga tgagttcctg ctcaatttgt gccagcgcaa atatcgcctc
     5821 acgtaaagca aaggttaatg ccggaccgac tttcaaaatt gcaaaatgat cgcgaactaa
     5881 ttcccgatag gccgtctgag tctggtaatc ggtggaatgc gcttcataaa ccattttggt
     5941 tttttctatc cattgcgcca acgcctgagc ttcttgcgcc tgataatgaa taatattgct
     6001 atggtcaaat tccacacccg gctgcaccac gatggcaatc acgcgtgtta acgcctctgc
     6061 cagaccgcgg gcaataaacg ctttttgatg cgtgcgtaaa gtattggcgg catcttcaac
     6121 ctgggtgatg tgtactgact gaatggcgct ggcctcaccg cccggaaccg gaacttcagt
     6181 gccaatgaca tagttcagtt gctcacgctg gcaatcagtc gccacacttt ccgccgccag
     6241 acaaagcaca gcggcacgtt cagcaaccgt ttctggcgct aacggtatgg aatcgtcagc
     6301 gcaggacatt gacgcatcaa gatgaatttt actgaagccg gcacgaacat atgcctttac
     6361 cagctcgacg gatttttcca tcgccgcatc cgcattttct tgctgccagc agtttggtcc
     6421 cagatggtcg ccgccgagaa taatacgctc acgagcaaat ccgactttat cggcaatcgc
     6481 aaaaacaaat tcgcgaaagt ctgccggtgt cattccggta taaccgccaa attgattgac
     6541 ctggtttgac gttgcttcaa tcagcacttt acgcgtgctg ttgcgatcaa atgccagcgc
     6601 cgcttcgata accaacggat gtgcagaaca gactgaacat atgccaatat gttcaccagc
     6661 tttatgccgg gcaattaacg ttttcatgtt ttttccttgt taaatggcag gtgcgttatg
     6721 ccctgccctc gcagccacaa tcggcaatca ctttgctcac cacatcgcgc attgcagatt
     6781 tagccgactg caaataatca cggggatcgg tcgcttcggg gtgctcggtc aggtaatttt
     6841 ttaacgcctg cgagaaggca ttttttagct ctgttgcaac gttgattttg catatcccca
     6901 gtttgatggt ttgctgaata tccttagtcg ataatcctga cgcgccatgc agcaccagcg
     6961 gtaaattcac ccactggcga atgttctcca gtcgagaaaa atcaagcgcc ggtgctctgg
     7021 catacatacc gtgagccgtg ccgatggcga ccgccaggga atcaatcccg gttgcctcgg
     7081 caaattcacg cgcctgaacg gggttggtat aaaaagcatc ggcttcattg acttgcacat
     7141 catcttcctg gccgccaagt tgccccagct ccgcttcgac gctgacatca aagcgatggc
     7201 aaaaatccac cacctctttg acccgtgaaa tattttgcgc aaaaggcaaa tgcgaggcgt
     7261 caatcatgac tgagcgcacg ccagaacgaa ccttctgagc gatatcgtca aattttgtgt
     7321 gatggtcgag atgaattgcc agtggatggt gatattgctt cgccatcgcg ctgaccagcg
     7381 ccatcagatt ttctgtaccg gcatgagtaa atgtgccggg cgttccggcg atgatgaccg
     7441 gcgcatgcat gctggcagcg gtttccacca ccacttgcat cgtttcgaga ttgtgaatat
     7501 tgaatgccgg aaccgcataa ccgccgcgct gcgcgttgtt cagcatctgc tttgtcgata
     7561 ccacgtacat tttcacatcc tgttgtttat tttcgatttc aaaacataat gaaattattt
     7621 gtttttaaat gtcgagataa cgatcacaaa aacgacaata tgaaaattgt tcgaggaatg
     7681 aaaggcaaaa aaaacggcct cccgggggaa gccgtaacaa agtacgcgtg gttttatgcc
     7741 ggatgtggcg ttaacgcctt ttccggccta cggggtagtg ctgttcgtat gaagcgcatc
     7801 aggcgatagt aattttgcta tcgagataaa cgtcctgcac ggcgttaatc agtttcacgc
     7861 cgtcagccat cgatttcttg aacgccttac gtccaagaat cagccccatt ccgcctgcgc
     7921 gtttgttgat aaccgcagta cgcactgcat cgctgaggtc agtttcaccg cctgcagcac
     7981 cgccggagtt tatcaacccg gcccggccca tatagcagtt agctaactga taacgcacca
     8041 ggtcaatcgg gttttcactg gtcagtttgc tgtagactcg atcgtcggta taaccgtaat
     8101 taatcgcttt atagccgccg ttattttccg ccattttttg tttgacgata tctgcaccta
     8161 tggttgccgc cagatggttc gcctgaccag tcaggtcggc ggaaacatgg taatcaacgc
     8221 catctttctt aaaggcggag ttacgcaaat aggcccacag cactgtcacc atgcccagct
     8281 cgtgcgcacg ttcaaaagcc gcagaaattt cttcaatctg gcgacgtgac tcttccgagc
     8341 caaaatagat agtcgcacca accgccaccg cgcccatatt gaacgcctgc tccacgctgg
     8401 catacagcgt ttgatcgtag gtattcgggt aacttagcgt ctcgttgtga ttaagtttga
     8461 cgaggaatgg aatgcgatgc gcgtaacgcc gcgataccga tgccagcacg ccgtaagttg
     8521 acgccacaca gttacagccc gcttcgatcg ccagttcaac aatgtttttc gggtcaaagt
     8581 agagcgggtt agcggaaaat gaagctccgg cagagtgctc aacgccctgg tcaaccggca
     8641 gaatagaaag atatcctgtg ccagccagac gcccggtgtt gtacaacgtc tgcatattac
     8701 gtaacaccgc tggcgggcga ttattgtcaa tcatcacgcg gtctacgtag tcatgtccgg
     8761 ggagataaag ctggtcagaa ggaatagtca tacaacggtg ctgtaaaagg ttgtcggcgt
     8821 ctttgccaag caactgcgca atatctgtca ttactatgct cccgtaaatt ccgattggat
     8881 atcggctatg gattgtcctg gcccgccttt tgcgggcaat cataatcctg gtcgttacgg
     8941 gcgagttttt ccatcttttg gatgtttttt cagcgttttc tgttggctcg attcatcaga
     9001 aaaaatgtta ccgcggtcaa gttttcaccg caaaggtatt taaaaggtat tgctaagtgg
     9061 tattgtcatc gcgtacctta cattacctgt catgaaggaa ttaaaagatg aaaacaacag
     9121 caaagctgtc gttcatgatg tttgttgaat ggtttatctg gggcgcgtgg tttgtgccat
     9181 tgtggttgtg gttaagtaaa agcggtttta gtgccggaga aattggctgg tcgtatgcct
     9241 gtaccgccat tgcggcgatc ctgtcgccga ttctggttgg ctccatcact gaccgctttt
     9301 tctcggcgca gaaagtgctg gcggtattga tgttcgctgg tgcggtgctg atgtatttcg
     9361 ccgcgcaaca gaccactttt gccggcttct tcccgttact gctggcctac tcgctaacct
     9421 atatgccgac cattgcgctg actaacagta tcgcttttgc caacgtgccg gatgtggagc
     9481 gtgatttccc acgcattcgt gtgatgggga ctatcggctg gattgcctct ggtctggcat
     9541 gtggattctt gccgcaaatg ctgggttatg acgatatctc accgacgaac atcccgctgc
     9601 tgattaccgc cggcagttct gctctgcttg gtgtgtttgc atttttcctg cccgacacac
     9661 cgccaaaaag caccggcaaa atggatatta aagtcatgct cggcctggat gcgctgatcc
     9721 tgctgcgcga taagaacttc ctcgtctttt tcttctgttc attcctgttt gcgatgccac
     9781 tggcgttcta ttacatcttt gccaacggtt atctgaccga agttggcatg aaaaacgcca
     9841 ctggctggat gacgctcggc cagttctctg aaatcttctt tatgctggca ttgccgtttt
     9901 tcactaaacg ctttggtatc aaaaaggtat tgttgcttgg tctggtcacc gctgcgatcc
     9961 gctatggctt ctttatttac ggtagtgcgg atgaatattt cacctacgcg ttactgttcc
    10021 tcggtatttt gctgcacggc gtaagttacg atttttacta cgttaccgct tacatctatg
    10081 tcgataaaaa agcccccgtg catatgcgta ccgccgcgca ggggctgatc acgctctgct
    10141 gccagggctt cggcagtttg ctcggctatc gtcttggcgg tgtgatgatg gaaaagatgt
    10201 tcgcttatca ggaaccggta aacggactga ctttcaactg ggccgggatg tggactttcg
    10261 gcgcggtgat gattgccatt atcgccgtgc tgttcatgat ttttttccgc gaatccgaca
    10321 acgaaattac ggctatcaag gtcgatgatc gcgatattgc gttgacacaa ggggaagtta
    10381 aatgaaaaca gaacgtattc tcggtgctct ttatgggcag gcgttagggg atgcgatggg
    10441 gatgccctcc gagctttggc cgcgcagtcg cgtcaaagca cactttggct ggattgaccg
    10501 ttttctgcct gggccaaagg agaataacgc ggcctgttat tttaaccgcg ccgaattcac
    10561 cgacgatacc tcgatggcgc tgtgtctggc ggatgcgtta ctggaacgtg aaggcaagat
    10621 cgatccggat ctgattgggc gtaatattct cgactgggcg ctgcgtttcg acgcctttaa
    10681 caaaaacgta ctaggtccga cctcgaagat tgcgcttaac gccattcgcg acggtaaact
    10741 cgttgctgaa ctggaaaata acggcgtgac caacggcgca gcgatgcgcg tctcgccatt
    10801 aggttgtttg cttccggcgc gcgatattga ctcttttatt gatgatgtgg cgctggcctc
    10861 cagcccgacc cataaatccg atctggcggt tgcgggggcg gtagtcatcg catgggcgat
    10921 ttctcgtgcc attgacggag aaagctggtc agcgattgtt gattcactgc cttcaattgc
    10981 gcgacatgct caacaaaaac gcatcactac cttcagcgcc tcactggcag cacgtctgga
    11041 gattgcgctg aaaattgtgc gcaatgctga cggcaccgaa tccgccagcg aacagcttta
    11101 ccaggtcgtt ggcgcaggta ccagcactat tgagtccgtt ccgtgcgcca ttgcgctggt
    11161 tgaactggca caaaccgacc cgaaccgttg cgccgtcctg tgcgctaacc ttggcggcga
    11221 cacagacacc atcggtgcta tggcgacggc aatttgcggc gcgttgcatg gcgttaatac
    11281 tatcgatcct gcgttaaagg cggagctgga tacagtaaat cagcttgatt tcaaccgcta
    11341 tgccacggcg ctggcgaagt atcgccaaca acgggaggcg atatgagcgg cgctcgatta
    11401 cacacgctgc tgcctgaatt aacctcgcgt cagccagtta tggtgattgg cgcggcggtc
    11461 attgatgtga tcgccgacgc ttatgccctc ccctggcgtg ggtgcgatat cgaactgaaa
    11521 cagcagagcg ttaacgttgg cggctgtgca ctgaatattg ccgtggcatt aaagcgcctc
    11581 ggcatcgaag cgggtaatgc cttgccgctc ggtcaggggg tgtgggcgga gattattcgc
    11641 aaccggatgg caaaagagga cttaatcagc ctgatcgata acgccgaagg tgataacggc
    11701 tggtgtctgg cgctggttga gcctgatggc gaacgcactt ttatgtcatt cagcggtgtg
    11761 gaaaatcagt ggaatcgcca gtggctggcg cgattaaccg ttgcgcctgg cagcctgctc
    11821 tatttttccg gttatcaact ggcctcgccc tgcggcgaat tgttagtgga atggctggaa
    11881 aagctgcaag acgtgacgcc gtttatcgat tttggcccac gtattggcga tatcccggat
    11941 gcattactgg cgcggatcat ggcctgtcga cctttagtgt cactcaatcg tcaagaggct
    12001 gagattgccg ccgaacgttt tgctctatcc gcagagataa caacactagg caagcaatgg
    12061 caggagaaat ttgctgcgcc gttgatcgtt cgtctcgata aagagggcgc atggtatttc
    12121 agcaacgacg cttctggctg cattccggca tttccaacgc aagttgtaga caccattggg
    12181 gcgggcgaca gtcatgccgg cggcgtactt gccgggctgg cctctggtct gccactggcg
    12241 gatgccgtat tactgggcaa tgcagtggcg tcgtgggttg tcgggcatcg gggcggtgat
    12301 tgtgcgccaa cgcgcgagga actactcctc gcacacgaaa acgtatagat cgctgcgaca
    12361 gtggctgatg ctgtactcaa taggccgttg ctgttggtca agcgccactt gcttgatcac
    12421 cagcaccggt attttgctat ctaactgaat atgtgactga aactcggcat ccggcatccg
    12481 ggcgctaacg cgggaacgcg tacgttgtgg gtaaatgtgc tggctgcgga agtagtcata
    12541 aagagagatc ccgatggcat caacatcatg aattaaatgt gccggaaccc acgattcctc
    12601 aatcgatacc gcctcttcat cgacatagcg aatacgctta agcaaaaaga catcacttcc
    12661 cgcttcgacc gccagctgct gcgcgacttc ttcgggacat ttcacgacac gcttgttaac
    12721 ccatagcgta tcgggctttt tcccgcgcaa taccacctgc tgagaaaacc cccgcgcttc
    12781 tttcagcgag tattcgaaga tgttgttgat ctgcgtgccg taaccacgcg aacgcgtcac
    12841 cacgccctct tcttccagcg cctgcatcgc cttgcgcacc gtaatgcgcg aaacgccggt
    12901 taactgactt aaatcacgct caccgggcaa aatattgcca tgctccagca ccccgttgcg
    12961 cacggcattt tttaccgttt cggcaaactt aatataaagc ggcgtgttat ccgccgctaa
    13021 aatacgctcg ttcagttggg caatcagctg ggtatgcgct tgttccattt gtctttttcc
    13081 tgccggggaa tctggtggga gtatatcact gcttcatatt taaggcgttt ctttaatccc
    13141 atcaacgaat ccctgcaact cctcctctgt gccattaaac acattaaaat ccaccggacc
    13201 attaatgcca tcgacctgtc cacggtcgga atgctgccag aagcgccagg ccatgccgtc
    13261 attgtccggg cgacgttgat aatagtgagc cacccaccac ggatattcat tgaaatagcc
    13321 cgccagattg gtgtgataaa aaacggctcc tgagtaaata attggctttt ttcccgtacg
    13381 tttttcgacc atttttagcc actgacttac ccgcttgcgt aattcttttg ccgataattt
    13441 tcctcgctct tctacgtcca gtaccgcagg gaaatcgcct tgtgagaaat ccaccgtttg
    13501 cagaaataat cgcgcctgaa ctgaagcgga tactgacggg gagaaataat gatacgcccc
    13561 gcgcagcagg ccattttcgc ggtttagttg ccagttacgc aaaaaatagg ggtccaccag
    13621 cttttcgcct tccgtcgcct taataaaggc aaattgtaag cgaataccgt tatcgcgcat
    13681 ttttgccacc cgctgccagt cgatccgctc ctgccagcgc gaaacatcta ttccgtgaat
    13741 ggtgtaactg gcaggaatgc gaatagcgaa agatttaacc gggcgataac cgtagaagtg
    13801 aatgtagtct ttaatctgaa ttgctgtcga atagaaaaaa ttcaccgtct gacgcggata
    13861 aattgcaacg atggaaatta gcccaagagc gcataataaa acggtgaatt tttttcgact
    13921 ggtgattctc aactgcattg gtttcatccc tgaatgtcag caccaaaggc tgacaataac
    13981 caaagcagct atagtacggt gctcaccacc aggcgtggaa gtggtgaacc ggaccaatac
    14041 cgtgaccaac ttccagcgtg tcggcctggg ctaatgccga tgaaagccag ctttttgcct
    14101 cctgtacggt gtcagcccag tttgtatggc gcgggcatag tgcagccaac gccgcagaga
    14161 gcgtacaacc agtgccgtga gtgtttttgg tcataatgcg ctgtgcggta aaccgttgtt
    14221 caccctcgcg ggtaaacagc cagtccgggc tttgctcatc atccagatga ccacctttca
    14281 ttagcactgc gccacagccc atcgccaaca gcgatcgccc ttgttccagc atttcctgtt
    14341 cggtgcgcgc gtgtggcgcg tcgagcaagg cggctgcttc gggcaagttt ggcgttatta
    14401 atgaaacctg tggcaataat cgactgcata gcgtggcaac tgccgaaggt gaaagcagcg
    14461 ggtcgccgct ttttgccagc ataacggtgt cgagtaccac gttttggatc tgataacgtt
    14521 gcaaccgttc tgccaccgct tcaacaatat cggtttccgc caacataccg attttagtgg
    14581 tgtcgattcg cacatcgctg aacaccgaat cgagctgggc ggcgacaaaa tcaggctcaa
    14641 tgcgatacac cgactgtacg ccacgggtat tttgcgccac tagtgcagta ataactgagc
    14701 aaccataagc gccaagtgcc gagaaggttt taagatcggc ctgaatcccc gcaccaccac
    14761 tcggatcagt accggcaatc gtcagagcgt taattcgttt catgcctgca cctcctgcgt
    14821 caattgccag agcgcatcaa ggaaatgtgg aacaaaactg cctggcccct cgcttctggc
    14881 aactgcgcgt tctccggctt gtttcatcca gtgacaggca gatgcgatat tttccagcgt
    14941 atcgcctggt agcgcacaac aggcagcgac aaccgccgat agtgcacagc cagttcctac
    15001 cactttggtc attaacggat cgccaccgtg aatgccaacg atacgatgtc catcggtaac
    15061 ataatccacc tcgccagtga ccacgacgat tgcgccagtt tcccgtgcca gtgtttgtgc
    15121 agcaggtatc acgttagctg cggcatcagt ggtatccact ccccgtccac cattagcaac
    15181 gccagctaat gccatgattt ccgaagcatt accacgtatg gcggttggct tatgggacag
    15241 aagttccaca caaaaacggc ggcgataatc gagcgcacca accgcaaccg gatcaagcgt
    15301 ccagggcgtt tgtgatcgtg ttgcttgctc aacggcagca ctcatcgctt gcgctcgtgg
    15361 ctgcgtcagc gtgccaacgt taatcaacaa ggcactggcg atagccgcaa actgactggc
    15421 ctcttcggtt tcgataacca tcgctggcga tgcgccgagc gccagcaagg tattggcagt
    15481 aaaagtttgc accacatcgt tggtcatgca gtgcacaaga ggggaatgtt ggtgaaaaag
    15541 gtgtaacgcg tgcgcagatt gcgctgaact cagcaggtcg acttgcatag tttgctcctg
    15601 ccataacgtg aagaagcaat gacctggtgg tccgtgactt ccctacgctg gcattatcca
    15661 gatcaggtga tacgggtatt tctcagcctt cacaaagaag ggcaccccga gtcgttaggt
    15721 tgcgataata aatcgcagag gaggatggta atgtccagcg cacgcgttgt aaacggggaa
    15781 aaacggcagt tttacaatcg cgttaataaa ttatttgata taagaatcca gcaccttcag
    15841 aacgacatcc agatcttctt ctcgttttag ctcatccccc tggtgaacga tgtgttccgt
    15901 cagatgacct ttaatcactt cccgcatcag accgtttacc gcgccacgga tagcagcaat
    15961 ctgttgtaaa actgcagcgc attcatgcgg ttcgtcgagc attttcttga gcgccacgac
    16021 ctggccctga atcttactgg cacgcgcttt cagtttctgt ttatcacgga ttgtatgaga
    16081 catggcaaca cctggttaac aagaatatga aaaatcatag cactattaat ctactggggg
    16141 gtagtatcag gtactggggg ggagtagaat cagattgccg aattaatact aagaattatt
    16201 atcatgaccg aatttacaac acttcttcag caaggaaacg cctggttctt catccccagc
    16261 gccatcttac ttggtgcgct tcatggcctg gaaccggggc actcaaaaac gatgatggcg
    16321 gcgtttatca tcgccatcaa aggcaccatt aaacaggcgg tgatgctcgg actggcagca
    16381 actatttcgc ataccgcagt ggtctggtta attgcctttg gcgggatggt gatcagcaaa
    16441 cgctttactg ctcaatcagc agaaccgtgg ctccagttga tttccgcagt gatcattatt
    16501 agcaccgcgt tctggatgtt ctggcgtacc tggcgcggcg aacgcaactg gctggagaat
    16561 atgcacgagc atgatcatga gcatcatcac cacgatcacg aagatcacca cgaccatgga
    16621 catcatcacc atcacgaaca tggcgagtat caggatgccc atgcacgagc ccatgccaat
    16681 gacattaaac gacgctttga tggtagagag gtcaccaact ggcaaatttt gctatttggc
    16741 ttaaccggtg gccttatccc ctgcccggca gcaattaccg tgctgttgat ttgcattcag
    16801 ttgaaagccc tgacactggg cgcaacactg gtcgtcagtt tcagcctcgg cctggcgtta
    16861 acgcttgtca ccgtaggcgt tggcgcagca atcagcgttc agcaggtcgc aaaacgctgg
    16921 agcggattta acactctcgc taaacgcgct ccctattttt ccagtctgtt gattggctta
    16981 gttggtgtgt atatgggcgt acatggcttc atgggcataa tgcgataact caatccgtta
    17041 ggctcgcatg ttatcttcag ccctggatga ttcttaaatc agctatttcc gctgactctc
    17101 tgcacgctaa ggatgctttt agggcatcct ttcatttaca ctttttacga aatcatggga
    17161 tcactaacaa aatatcgctt gtcagttata ttgtatggca ggaaagatat gcgactgata
    17221 ttacagatcc ccaaagtgga gagtttatga ccattaaaaa taagatgttg ctgggtgcgc
    17281 ttttgctggt taccagtgcc gcctgggccg caccagccac cgcgggttcg accaatacct
    17341 cgggaatttc taagtatgag ttaagcagtt tcattgctga ctttaagcat ttcaaaccag
    17401 gcgataccgt cccagagatg taccgcaccg atgagtacaa cattaagcag tggcagttac
    17461 gtaacctgcc cgcgcctgat gccgggacgc actggaccta tatgggtggc gcgtacgtgt
    17521 tgatcagtga caccgacggt aaaatcatta aagcctacga cggtgagatt ttttatcatc
    17581 gctaaaaaaa agccccctcg gagagagggg ggaatgcaga caccttaata tttttattat
    17641 tagccacttg ctcgtcttgc ttgttattag tcgtatttca cgttgattaa tgcggttgcc
    17701 tccagtgcgc cagatttaac tttgtttgta tcgtagacgt agtaactggc cgttatcgga
    17761 attgaaaccg ttgtatctgt aagttttgac tttgccgcaa tttcttttaa atttgtatat
    17821 cctttactgc tgttagtgat atttaaaagt gtgactggcg taccattact ttcaagaata
    17881 aatcctacgc cattatcttc cgcacctttc aggatcgtgt tgctgttggt taacaagtca
    17941 gaacttgata aatacacctg cattttccgt gatgtttgat tttgttgacc t
//
LOCUS       DADTFG010000099        17645 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.099, whole genome shotgun sequence.
ACCESSION   DADTFG010000099 DADTFG010000000
VERSION     DADTFG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.099"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(138..1010)
                     /locus_tag="J1138_003138"
     CDS             complement(138..1010)
                     /locus_tag="J1138_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBA8454904.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGCEYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(1022..2116)
                     /locus_tag="J1138_003139"
     CDS             complement(1022..2116)
                     /locus_tag="J1138_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBA8454905.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(2149..3147)
                     /locus_tag="J1138_003140"
     CDS             complement(2149..3147)
                     /locus_tag="J1138_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8454906.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFSVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(3172..4683)
                     /locus_tag="J1138_003141"
     CDS             complement(3172..4683)
                     /locus_tag="J1138_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA8454907.1"
                     /translation="MFTVTEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(4706..5689)
                     /locus_tag="J1138_003142"
     CDS             complement(4706..5689)
                     /locus_tag="J1138_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBA8454908.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(5786..9067)
                     /locus_tag="J1138_003143"
     CDS             complement(5786..9067)
                     /locus_tag="J1138_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBA8454909.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQSVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSDRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIILSYQEHQPQVLPLPDVAKA
                     PLAAQDITSTDETWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAVRNGNFDTGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVVKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            9185..10378
                     /locus_tag="J1138_003144"
     CDS             9185..10378
                     /locus_tag="J1138_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA8454910.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(10442..11695)
                     /locus_tag="J1138_003145"
     CDS             complement(10442..11695)
                     /locus_tag="J1138_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBA8454911.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            12023..13213
                     /gene="hmpA"
                     /locus_tag="J1138_003146"
     CDS             12023..13213
                     /gene="hmpA"
                     /locus_tag="J1138_003146"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBA8454912.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPNEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(13258..13596)
                     /gene="glnB"
                     /locus_tag="J1138_003147"
     CDS             complement(13258..13596)
                     /gene="glnB"
                     /locus_tag="J1138_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBA8454913.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(13657..14991)
                     /gene="glrR"
                     /locus_tag="J1138_003148"
     CDS             complement(13657..14991)
                     /gene="glrR"
                     /locus_tag="J1138_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBA8454914.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKLRPLGSNRDIDIDVRIISAT
                     HRDLPKAMTRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(14981..15694)
                     /gene="qseG"
                     /locus_tag="J1138_003149"
     CDS             complement(14981..15694)
                     /gene="qseG"
                     /locus_tag="J1138_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBA8454915.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTARKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(15859..17286)
                     /gene="qseE"
                     /locus_tag="J1138_003150"
     CDS             complement(15859..17286)
                     /gene="qseE"
                     /locus_tag="J1138_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBA8454916.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNSLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
BASE COUNT         4213 a         4913 c         4403 g         4116 t
ORIGIN      
        1 aacgccttat ccggcctacc gctccaacac cgattgtagg cctgataaga cgcagcaagc
       61 gtcgcatcag gcatcgtgca ccgagtgcta caaaatcgaa attaccccgt aggcctgtgc
      121 atccggcatt ttataaatta cagcaacgca acccgcaacg acatctctga aattgttgat
      181 tcccctggtg caagcgcaat gaggtcacca ccttccggac gatgatggtc atccggcgca
      241 tggctcatcg gttccagaca gaagaaatca aacgcatatc ctttatcaaa cgcagggtct
      301 gaaacaaaga tgaaataaca cggtgcaggt ggcgtcgttt ccatgatgat ggcataaccc
      361 tcctgcggct gctcgatgcg ggccttacca ttccatccgg caaaaccatt gtttacccac
      421 tggcgcggca acggcgcgag ctggttaaaa tccagctcct gcggtagctg ctcgcaaaac
      481 tcacccgcca gccactgctc ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc
      541 gtttgcggcg acaacggaaa ataaggatgc cagccggtac caaatggcag cgtctctgcc
      601 ccttgattgg tgacagcgag cgtcaccgtc agcgtatccg ccgttaaatg aaatgcctgg
      661 cttacccgat agtgataaac accgctgaga tgttcataca ccagacacaa gctatcatcg
      721 ctgcgcgaga cgcattgcca ctcgcccaac cagccatcgc cgtgtagata atgcgcatcc
      781 cactcaacat tcggttgcag ttgatactca cacccctgcc agacaaaacg gttgccgctc
      841 acccggtttg caaatggtac cagcggaaaa caagaggcat cggttgccac accgcttttt
      901 ttaccaggac gtagcagcgg cgtcgtatcg cgccagaagc cttcgattac gccgccctga
      961 tcagaaacgt ccagctttag cggcccgtga gataaggtat agatggtcat ccgcagactc
     1021 cttaatccgg gaagttaatc acaactttcc cgcatttacc gctcgccatc agcgcatagg
     1081 catctcctgc ctgttccagt gagaagcgat gggtaatggc attacgcggc cacagtttcc
     1141 agtccgttaa atcgtgggcg catttttcca tatggaacag actggtcacc caggagccaa
     1201 taatccgccg ttgatgatgc atcagatcag cgctaacctc gaactccact tttccggttt
     1261 ccccgatgta aaccacccgt ccccagtcag cagtggattg caacgccagt aagcgacctg
     1321 cggcattacc ggaacaatca agcgcaacat ccgcgccgcc gtgagtgagt tcggcgatta
     1381 tctgcggcag accttcggtg gtcgctaaat agccgtgatc catcactcct aactgtttcg
     1441 ccatcgccag acgttccggc aacatatcaa cgccgatgat cctttttgca ccgcgacctt
     1501 tcgccagcat catcgccatc atgccgaccg gccccagacc gaccaccagt acgttatcac
     1561 tgccggaaac ttcgccgcgc aaaatccctt catacgccgt accaacaccg caactgataa
     1621 acgcgccatc ttcgtaactc agcgcgtctg gcaggaggat cagatctttt tcttccgcca
     1681 gtaggtattc ggcatgaccg ccgtcacgct gccagccgta agccgctttt ccttcgccag
     1741 tacaagaaat aggaaaaccg cgacggcagt tcgggcaaaa accacagcca gaaatgtgat
     1801 acaccagcac gcggtcgccc tctttaaaat ggcgacagcc ttgccccatc gccacaatct
     1861 gcccgcacgg ttcatgaccg ttgataaatc cctggtataa cggtttatcg ggtgccgccg
     1921 ccgtagcgcg gtgctggtgg tagatatagt ggacatcgct tccgcaaatc ccggaggatt
     1981 tcattttgat cagtacctgg ttaatccccg gcgtcggcac cgcaacttcc cgcagatcga
     2041 cggtcgaatt tcctggtaaa taagctgcca gcatcgtttt cataaatccc tcattaacaa
     2101 tacgattaat tttcatccct gccgcacccg cgccaggggc gttaaggttt agcgtttcgc
     2161 tttactgcta cgctgggtca gcaagatatt cgccagcacc gccaccacga tgatgacgcc
     2221 gcgcaccacc tgctggaaaa aggagttaat cccgagcagc accagaccgt taccgattag
     2281 cgtaatcacc agtacgccaa gcagcgtacc gaacaacgag ccgcgaccgc cggaaagcgc
     2341 cgtaccgccg accacgaccg cggcgatgac gtcaaactcc agaccgtttg cggcacctgc
     2401 gttaccggaa ccgaggcggg ccgccaacaa aatgccggtc accgccgcta ataatcccga
     2461 aagggtaaaa ataagaatgc gtacccgacg aacgttaata ccgcacaact gcgctgcagt
     2521 ggcattaccg ccaacagcaa aaaccgagcg cccgaaggca gttttgcggc taatgaacac
     2581 aaacagcgca aataacacca tcatgatcag cgcggatacc ggcacaccga gaaattgtcc
     2641 gcccagccag tccagcactt cgttttcgtt aattggcact ggcaaggcgt tcgtcataaa
     2701 cagccccatt ccgcgcaggg cgctccataa acccagcgtg gcaacaaaac ttggcacgct
     2761 aaacacgccg cgcagcaccc cggcgagcgt tcccatcagc gcacctaaca gcaacaccag
     2821 cagacaagca atcgccagcg gaacgtcaaa ttgcagcaaa aacgccaggc acactgaaac
     2881 aaaagccacc atcggcccaa cgctgacatc aatttcaccg gagataataa tcagcgtcat
     2941 cgcccaggcg gcgatcccaa tggtggcggc atcgcgcagc acgttcatct ggttattcaa
     3001 tgagataaag ccaggcgcat tcagggagaa gaccagataa agaatggcaa tcaccaccag
     3061 caaaccgatc tcattaatat ggcgactgac aaattgtttg agcgagacgc tcttgccctg
     3121 cggcaatggt aatgacgaag cagacatctt caggttcctc ggtaaaattc atcagtgcac
     3181 agacagaatg gcggacatca gctcatccac attgaccgga gagtgaaact cctgcgagaa
     3241 cgtgccgtgc tgtaataaca ggatgcggtc acacaccagc ggcaactcct ccacttcact
     3301 ggagataaac accacgcttt ttccttcggc agccagctcg cggacaatac ggtaaatctg
     3361 ctgtttggct tcgatatcga cgccgcgcgt tggctcgtcg agtaacaaaa tctggctggc
     3421 ggcatagacc caacgaccga tcaccacttt ttgctgattg ccgccagaaa gcgtgccgat
     3481 gggcgtttcg ctactggcgg ccttgaccgt catccgctgc atcacctctt cggtcaggcg
     3541 gcggatggtg gaccattgca gtacaccgtt ggcgctgatt ttttgccgat tggtcagcac
     3601 tgtattttcg tcaacgccca accagggaat gatccccgct tctttgcggt tttctggcgt
     3661 atagccaatg ccgcgtttca gcatgtcgcc gtaatcgggg cgcgtgattt tctcgccgtt
     3721 gataacaatt tcgccctgtt catacgtctc cagcccaaca atcgccttca gcaattcact
     3781 gcgccctgcc cccagcagac cagcaatgcc gagcacttcg ccacgacgta gtgtaaaact
     3841 gatatcctcc agcttgggct tatggcgtaa cgcacggact tccagcacgg cctgatccat
     3901 aatttcctga ggggcaaccg gcgcaatatc aacgtgatcg cgcccgagca ttagcgacac
     3961 aatatgatgc gtagaggtgt tttcgagcat cacatcgccc gccacctgac catcgcgcat
     4021 aacggtggca caggaggcaa tgcggcgaat ttcttccatc cggtggctga cataaatcac
     4081 cgccacgccc agtgccgaca tctttttcac cgcgctgatc accagttcaa cttccgcact
     4141 ggcaagcgag ctggtgggtt catcaagaat gaccacgcgc ggctcgccct tcattacccg
     4201 cgcaatttcc accagctgct tttgcgccgg gcttagtgtt gaaacaagtt gttcaggact
     4261 aacgtcaacg cccaacgcct gcaagcaacg ttgggcgtcc tgcgccattt gcaggtaatc
     4321 aatcatgccg ttgcggcggg gccactgacc gaggcagagg ttttccgcca ctgtcagccc
     4381 ttccaccaga cttaactcct gataaaccgc acgaaccccc agttcagcgg cacggcgagt
     4441 cagcgtagct tcgtcacctt ccagtcgcgt ctcgccaatc cagatatcac cgctatccgg
     4501 acgttcgcta ccggtaagca ttcgaatgag ggtcgatttt cccgcaccgt ttttgcctaa
     4561 cagcgcacga acttcgcctt tattgagcgt gaagttaacg ttatccagcg caacgacgcc
     4621 ggggtaacgc ttatttcctg ccaccacttt tgctaccggg actgcctctg ttaccgtgaa
     4681 catagggacc tctgcgaatc agcgattagg gcagaccatc aacgtgcgtt gccagccact
     4741 gtttaccgtc ttccgttttg gtatagagat cgattggcac ctgaatcact ttttcaccgt
     4801 cggcttgttt attgataacc ttcaatgttt gcgtaaaaac agcattgccc attttcttac
     4861 cggaaatatc caccaccgct ttcagcacct ggttgttttc cagctcctga gcaatttcag
     4921 tggtcatatc agaaccgaaa acagcaattt ttccggcctg attttgatta cgtaccgctt
     4981 ttaccgcgcc gagtgtcgct ccgcccgatt cccccataat ggcgttgaga tccggcgtgg
     5041 agataatcag tttttcaccg acagaaatcg ctttatctaa aacagtccct tcctgattag
     5101 cgacaatttg cgcgccggga acgcgggctt ttaatacttc ttcaaaccct ttacgtcgtt
     5161 gcacacaaac ttcaaaggct tcgcaattga tgacggcaat tttcggctgg tcaattttgt
     5221 tggcaataaa ataatcggcg gcagcgttac ccagtttttt accaaattcc agcgggtcac
     5281 caaccagata tgccgagaca tatttatcga cacccttttg attaatacag gtgttgtagc
     5341 aaatcactgg aatgcccgcc tcactggcgc gacgaacggt gcggctgctg ccattttcag
     5401 aaacagctga caaaataatg gcatcgacat tacgcgccac gagggtatca acaaaggtac
     5461 tttctttcga aatatcaccc tgggcgttag tttcaattaa ctgtacctgt actgaagaat
     5521 cttttgccgc atcctgaacg ccctgacgca ctccggcgta atatccctgg gtatcaaggt
     5581 atattgcgcc aatagtcatt tctttatcag aagccctggc aaatagtgcg ctacccaata
     5641 gcgtagccat taataataaa ttacgggttc ttgtcatttt tttaggcata gtgcctcctg
     5701 tagggttttt atttacaacg gcttatttta attatttgag cgtgagcggc agcgcgtgcc
     5761 gctcaaatat tacagttaat agggtttagt gaacgtagct aaaaataaaa ggcatcacgg
     5821 tggtgaataa tgccgctttc ggccaggctg gattaatcgc catcaattca gcgcgagcag
     5881 attcatagtg cgcaacctcc cctaatcccg cggccgccag catcgccacc atcaggcatt
     5941 tttctttatg ctgctgttgt aaatcgccat acagcgacaa caagtcaggc tgcgagacgg
     6001 caaagaaatc cgcttcgata ctggttttcg ccatctcttg tgcccactgt ttcatttcac
     6061 taaacagttg ctgtgcggtg tgttgttcgc ccagcagtcg cagcgccatt ccttgccaga
     6121 agagataatc aaccggctga tcgttgtaat aactgtggat gttaatggtg cgatcgccgg
     6181 tcgccgccag acgtaaacaa cgcgtcgctt cagtttcatc gccctgcgcg ttggcgcata
     6241 ctgcctgcca gaaccagatg tcgttgtcag tttgccccgg taaacggcct tcgcttaaat
     6301 tctccggata atgcagcgcg gcatgaagca gttcgctggc ctgctgcggc tctctggcat
     6361 caagatgctg ccaggcgcgt aataactggt tgaggataaa ctggctggtg accttccctt
     6421 ccccaccttc ccacgggtgg aatttgcgcg tggcgagaat gtccgccgct ttgtctgcct
     6481 gacccgtgag atgccacaaa ttgagcagtt ctgcggtcat gtcgtcgcgt ttcagcgcaa
     6541 tttccagatt attttccaga cgcgccagtc gtttctccgg tgtgacaccg cttaacttat
     6601 ccagcaaatc ccgttcgaaa agcagacgtg catcctgcgg cgcaagctga taagcattat
     6661 caagataacg cgcggccagc tcataatcgt gctgcttatt ccacgcatgg atcgccaacc
     6721 cgcgccagcc gtcggcaaac tccggcgaca tctctacgca acgctgccat aaggcaatgg
     6781 ctttgccgta gctacgcttg ttgtagtaga agcaggccag taaatgccgg gcaaaccagc
     6841 actcttcgat actctccagc gccgccactt cttccagcgt attcgggaaa cggacaaact
     6901 gcgggaaggc atcaatagct tttacgacca gttctccgcg ttcagctttt ggtagcaaac
     6961 tggcttgcag gtaaagcggt agcgtgcgct gacagtccag tgcgttcagc atctctgccg
     7021 ccagggcggg catgccccag ttaatcagtt gcccggcggt catcagggcg ttaacgtcgc
     7081 gtccctgaca aagacctcgc cactgcgcga gggctgattc gctacgacca tcgaaccagt
     7141 tcagccacca cagagtggcg ttcagcggat aatcacgcag cagtttctcg cgctgcaaac
     7201 gcgcgttgtc ctgacgacca cttaacacca gtagcaggtt atgcaggcaa agcacttcct
     7261 gattggttgg gcaggcgcga agactttgtt ggcaaaaatc cagaccagtg tcgaagttac
     7321 cattacgcac cgccagtcgt gccagaccat aatagccacc ggctttactg ttgccgctcc
     7381 agaccgcacg ccagaaatcc tcttcggctt gttgatattg cccctgacgt tcgtaagcac
     7441 tggcgcgaat caaactcgcc tgtccgcact gcggattttt gttcagcgca tgtgcgcgtt
     7501 tcagtgcctg actggcatat accaccgctt gcgggaaatc tgcacggtta tattccagca
     7561 ttgccagcgc cagattacag cgataatcca gcggatccag cgccacgccg cgcaggtagt
     7621 aatcgaacgg tgaacggctc gcgtgatgat attgctccag atgttgaccg ataaaccagg
     7681 tttcatctgt actggtaatg tcttgtgctg ccagtggcgc tttggcgacg tccggcaacg
     7741 gcaacacttg cggctgatgt tcctgataac tcagtatgat attgccgtcg gcatcggaga
     7801 gctcaatggt cagcctttca ggattgatac cgtgcaacac gccctggatg gcggtcgctg
     7861 gcatcagtgc cacggcatca tcaagtaacg cgttgcattt gccgatttcg cggatcgcca
     7921 ggcggtatcc gttcaacgga gagatggcat acaaccccca ctcaatcccc cgatcactac
     7981 gctggagttt tatcaccgca tcgcgggagg cgttttgcac catgcccaaa gaatggtaag
     8041 gcaggaaata ctgctcaaaa cgcttctctt cataagcatc aagccaggta aaatcgggct
     8101 gattatccgc aaaaatgccg gtcatcagtt cgatatacgg gccgttattg tcggtcagac
     8161 tcttatccca cgcctggcca aattcactgt gtccccagct ccactgtttt ttacctggcg
     8221 cgatatggtg gttggcaacg tgtagcaaac cgccatcttc atcgtgacac cacgcgccaa
     8281 caaaatcgta ctgtgatttt tcagccatat atgaggttgg aacgggcaca tttttatagc
     8341 gagaaatgtc cactccggcg gagtagtcca ctttgtagta agtgccggtg gcgatgggga
     8401 aagcagagac ggcccgtttg ccgtgatcaa acaccgccgt tacatccggc gggaagacgc
     8461 tctgatgccc ttcaccccct ttcactgccg ggttggccca ccacaagaaa tgacgcggcg
     8521 tggcgttgcc gttatagacg cggctggcaa tttccagtgc cgcccggtca gggcgcaggg
     8581 tgaaacctgt catcacctgt aaaccgtgca tcggctccgt ttcgccgacc cacacggttt
     8641 gtgcgccgtc ttcatgggct tcgagggtga aatcgacggg cataaaggtg gtcgggcgat
     8701 ggtgttgcgg ccagttaaac tcaatcccgc cggagatcca cggccccagc agccccacca
     8761 gcgccggttt aatgacttca ttgtgataga caaaatcgcg ctgtttcact ttatcccatg
     8821 cgcgatgcac ccgaccgccc agttccggca ggatcatcac tttgatgtag tcgttttcca
     8881 gccacaccga ctgccaggat ttcagggttt tctgctcgct cagcgtatcg gtcacgccgt
     8941 agggataaac cgcgccggac gatccctgat aaacgcgatt ttccaggaac ataggatgta
     9001 tatcctgcgg cccggtttca taggtcggga tctcaacgcg ctcttgccac acttttactg
     9061 gagtcatggt gccctcaata ttaacaagac atactgaatt aaaagatttg tggcagtgta
     9121 ttgaacaatc tggcaatgtt ttcgcggaat aatcacgcaa ttaactaaac aaggtttagt
     9181 gaagatgaga gcctgcatta ataatcaaca gattcgccac cataacaaat gcgtgattct
     9241 ggaactgctg taccggcaaa agcgcgccaa taaatcaacg ctggcccggc tggcgcaaat
     9301 ttcgattccg gcggtcagta atattttgca ggaactggaa agcgaaaaac gggtggtgaa
     9361 tatcgacgat gaaagccaga cgcgcgggca tagtagcggt acatggctga ttgcgccgga
     9421 aggtgactgg acgctgtgcc tgaacgtgac gcccaccagt attgagtgtc aggtcgctaa
     9481 cgcctgttta agtccgaaag gtgaatttga gtatttacag attgatgcac cgacgccgca
     9541 ggcgctgctg tccgaaatcg aaaaatgctg gcatcgccac cgtaaattgt ggccggaccg
     9601 caccatcaat ctggcgctgg caatccacgg tcaggttgat ccggtgactg gcgtgtcgca
     9661 aaccatgccg caagcgccgt gggcaacacc ggttgaggtg aagtatctgc tggaagagaa
     9721 gctgggcatt cgggtgatgg tcgataatga ctgcgtgatg ctggcgctgg cggagaaatg
     9781 gcaaaataat tcgcaagtgc gtgatttctg cgtaatcaac gttgattacg gcattggctc
     9841 gtcgttcgtg attaacgagc aaatttatcg cggcagtttg tatggcagtg gacagattgg
     9901 ccacaccatc gttaatccgg atggcgtcgt ctgcgactgc gggcgttatg gctgcctgga
     9961 aaccgtcgcc tcgttaagcg cattaaaaaa acaggcgcgg gtatggctaa aatcacaacc
    10021 ggttaatact cagcttgatc ctgaaaaatt gactaccgcg cagttaatcg ctgcctggca
    10081 gagtggagaa ccgtggatca ccagttgggt tgaccacagt gccaacgcca ttggtttgag
    10141 tctgtataac ttcctcaaca tcctcaatat taatcagatt tggttgtacg gtcgcagttg
    10201 tgcctttggt gagaactggc ttaataccat tattcgccag acgggattta acccgttcga
    10261 ccgcgacgaa ggaccgagcg tgaaagcgac acagattggc tttgggcaat taagccgcgc
    10321 acaacaggta ctgggaattg gctatttgta tgttgaggcg cagttacggc agatttgatg
    10381 gcgcgataac gtagaaaggc ttcccgaagg aagccttttt tcaacagatt aaccgtttcg
    10441 cttatgcgta aaccgggtaa cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga
    10501 taacggcttc atcattgata ctgtccagca cgtcacacat ccagccagcc agttctttcg
    10561 cttcggcttc tttaaagcca cgacgggtaa tcgccggagt acctacacga ataccggaag
    10621 tcacaaacgg gctcttcgga tcgttcggta cgctgttttt gttcacggtg atgttagcac
    10681 ggcccagagc ggcgtctgct tctttaccgg tcaggttttt atcaaccaga tcaaccagga
    10741 acaggtggtt atcagtacca ccagaaacca ctttgtagcc gcgttcgagg aacacttcta
    10801 ccatcgcttt ggcgtttttc gcgacctgct gctggtaagt tttgaactca ggttccattg
    10861 cttctttcag agcaaccgct ttaccggcga ttacgtgcat caacggaccg ccctgaccac
    10921 cagggaaaac ggcagagttc agttttttgt acagctcttc gctaccacct ttcgccagga
    10981 tcaggccgcc gcgcggaccc gccagggttt tgtgagtggt ggtagtaaca acgtgagcat
    11041 gaggaaccgg gttagggtag acaccagcgg caaccaggcc cgcaacgtgc gccatatcaa
    11101 cgaacaggta agcaccgatg ctgtcagcga tttcacgcat tttcgcccag tcaaccacgc
    11161 cggaatatgc agagaagcca ccgataatca ttttcggttt gtgttctttg gcttgttttt
    11221 ccagatcggc gtagtcgata tgaccggtag catcgatacc gtaaggaacg atgttgtaca
    11281 gtttaccgga gaagttaacc ggagaaccgt gagtcaggtg accgccatgc gccagattca
    11341 tacccaaaac ggtatcgcct ggttccagca acgcggtgta gaccgcaaag ttagcctggg
    11401 agccggagtg cggctggacg ttagcgtagt cagcgccgaa cagttctttc gcacgatcga
    11461 tcgccagttg ttcaacgata tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat
    11521 aaccttcagc atatttgttg gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg
    11581 tgtagttttc ggaggcgatc agttcgatgt gctcttcctg acgtactttt tcctgctcca
    11641 tagcctgcca cagttcggca tcataatcgg caatgttcat ttcacgcttt aacatccgca
    11701 tctcctgact cagctaacaa taaaattttt ggcctttata ggcggtcctg ttggacaacg
    11761 gcgaacagta taaccgaatc attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa
    11821 cgattacctt caggctacgc aaggctttgg agaataaaga gcttgcaacc ggaaacggat
    11881 ttcttttcag gtttgtgatg caaatttttc acttcatcac attctttctg aaaaacacca
    11941 aagaaccatt tacattgcag ggctattttt tataagatgc attttagata catcaattaa
    12001 gatgcaaaaa aaggaagacc atatgcttga cgctcaaacc atcgctacag taaaagccac
    12061 catcccttta ctggtggaaa cagggccaaa gttaaccgcc catttctacg accgtatgtt
    12121 tactcataac ccagaactca aagaaatttt taacatgagt aaccagcgta atggcgatca
    12181 acgtgaagcc ctgtttaacg ccattgccgc ctacgccagt aatattgaaa acctgcctgc
    12241 gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc agtttccaga tcaaaccgga
    12301 acagtacaac atcgtcggcg aacacctgtt ggcgacgctg gacgaaatgt tcagcccggg
    12361 acaggaagtg ctggacgcgt ggggtaaagc ctatggcgta ctggctaatg tatttatcaa
    12421 tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc ggtggttggg aaggtactcg
    12481 cgatttccgc attgtggcta aaacaccacg cagcgcactt atcaccagct tcgaactgga
    12541 gccggtcgac ggtggcgcag tggcagaata ccgtccgggg caatatctcg gcgtctggct
    12601 gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac tctttgactc gcaaaccgga
    12661 tggcaaaggc tatcgtattg cggtgaaacg cgaagagggt gggcaggtat ccaactggct
    12721 gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc gctccggcag gtgatttctt
    12781 tatggctgtc gcagatgaca caccagtgac gttaatctct gccggtgtcg gtcaaacgcc
    12841 aatgctggca atgctcgaca cgctggcaaa agcaggccac acagcacaag tgaactggtt
    12901 ccatgcggca gaaaatggcg atgttcacgc ctttgccgat gaagttaagg aactgggtca
    12961 atcactgcca cgctttaccg cacacacctg gtatcgtcag ccgaatgaag ccgatcgcgc
    13021 taaaggtcag tttgatagcg aaggtctgat ggatttgagc aaactggaag gtgcgttcag
    13081 cgatccgaca atgcagttct atctctgcgg cccggttggc ttcatgcagt ttgccgcgaa
    13141 acagttagtg gatctgggcg tgaaacagga aaacattcat tacgaatgct ttggcccgca
    13201 taaggtgctg tgatttgatg ttgccggatg gacacatccg gcaacccttg acccgtttta
    13261 aatcgccgca tcatcctctt caccggtacg gatacgaatg acccgtgcca cgtcgaagac
    13321 gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg tatcgacaca
    13381 ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc actttcggca gaaaatccac
    13441 catatactcc gcaccgcggt acagctcggt atggcctttc tggcggccaa aacctttcac
    13501 ctcagtgacc gtcatgccgg taataccgac ttcagccagt gcttcgcgaa catcgtccag
    13561 cttgaagggt ttaataatcg catcaatctt tttcatgcta ttccttgaaa aggtcgcctg
    13621 tcttttgatc tgctaaacgt aacacataac gccaattcat tccttgaaat cgtttgcatc
    13681 cagctcgtgt cgggatagca gtttataaaa ttctgtccgg ttgcgccccg ccattctcgc
    13741 cgcgtgggtg acgttgcctt tggtaatttg cagcagctta cgcaaatagt tgagttcaaa
    13801 ctgattacgt gcctcaacaa aggttggcag cgccgtattt tcaccctcca gcgcctgctc
    13861 caccagcgca tcactaatca ccggagatga ggtcagcgcc acgcactgtt caatcacgtt
    13921 gaccaactgg cgcacattac ccggccagct cgcggtcatc aggcgtttca tcgcatcggt
    13981 ggagaacgcg cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca ggtgatttgc
    14041 cagtagcgga atgtcttctg tgcgctccgc cagggccgga attttcaggc tgacaacgtt
    14101 gaggcggtaa tagaggtctt cacggaattc cccgcgcgtc atcgcttttg gcaggtcgcg
    14161 gtgggtcgca gaaataatcc gcacgtcgat atcaatatcg cggttactgc ccagcgggcg
    14221 caatttacgc tcctgcaaca cgcgcagcag tttgacctgc aacggtgcgg gcatatcgcc
    14281 aatctcatcg agaaatagcg taccgccttc cgccgcctgg aataaacctt cgcgattgct
    14341 gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc tccgactcca gcaattgctc
    14401 gggcaatgcg ccacagttaa tagcaataaa tggtttgctg ttgcgcgggc tggcgttgtg
    14461 gatagcctgg gcgaaaatct ctttcccggt gccgctctgg ccgttaatca aaacgctaac
    14521 gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg ggctgcgggt
    14581 gacaatcgcc tcgcgccagc gttcgtcggt ggctggcgcg gattgttcca gcgcatcgtc
    14641 aattgcctga tatagcgcat ctttatcgac aggcttggtg agaaaactga acacgccctg
    14701 ctgtgttgca gcaacggcat caggaataga accatgcgcg gtaagaataa ttactggcat
    14761 tcccggctgc actttctgga tttcagcaaa cagctgcata ccgtccattt catccatccg
    14821 caggtcgctg atgactaaat ctactttttc gcgattaagt acccgcaatc cttcagcacc
    14881 actttccgcc gtgaccacac tgtagccttc gctggtcagg cgcaggccaa gcagtttcag
    14941 caatcccgga tcgtcatcga ccagtaataa atgcgcaggt ttatggctca tcaggagtga
    15001 cctcatcggt ggatggcgcg ggcttttcac tctcgtgcgg cgtatccggc gagaaatttc
    15061 cagccggttt gcgggtcgag agctggcgtt caatatcggt caggttttcc agcttgcggg
    15121 cagtgagttc cagttgctgt tgtagaacgt gatgttgctg gcgcaaagta tccagctcgc
    15181 tgtcgctgga ctgctggagt ttgctgtagc gttggcgctc ttccgccagt tgcagttgca
    15241 gcgcctgacc gtcgcgccaa agttgataca gtgggcgaac ctgtgccggg atctcggtac
    15301 ttagcgcatc aatgcgcgcg accagctggc ggcgctcata cggcgtaatt ttggcgtcgg
    15361 cgagcaaaat cccttgttta aaggtatttt gccagttgcc gtcgtcatat tggcgggctt
    15421 gctgacgcga ctgcgcaggc attaaacgat cagcacaatc catcgcccgc agccagtaaa
    15481 gcggattggt ttcggttgat ttaccttgca gcgcccagat gtcgctacat tcagtagaaa
    15541 gatagtcagc cagttgataa accggaattt tttcttctgc tggcgtatca atggctggct
    15601 tattgtgact ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc agccacagcc
    15661 gtcggggcaa taatcgttga aaaatgtgtc gcatattcac cagacttaaa gcctatccca
    15721 gtgggcgtaa ttgttgcagg cagtttggac atggacagcg cggagaaacc ggagcgtaca
    15781 tgtcgtacgt gaagatttcg agcactgccc ggggccaaac tggcaaataa aatagcctga
    15841 tgggataggc tgttagcatt atttcgtgtt tttcgacgac ggtaattcaa tgcggaaaca
    15901 aacatcttgc ccgctctcgt cgaccagata cagttcccct tgcatacggc gaatacaatc
    15961 cctggcaatg cttaatccca gaccgctgcc cttcaccgcc ccttttcgct ggtggcttcc
    16021 ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggcg tgcctgtatt
    16081 gatgacatca atataaaccc gcgcaccatg taaactgctg cgaaggcaaa tgttaccgga
    16141 ttcagccccg tagtgcaccg cattggagta aagattatcc agtacgctca tcagcagcat
    16201 tggctccgcc aggcaagctg ttgctttgag atcaacgtcg gtatgcatca ttttagcccg
    16261 tgcgggcagg ctatgagcag aaaccactgt ctccaccagc ggtgctaact caacattctc
    16321 cagttccacc gcactgtccg cctgtttacg gttgtaatca agcagttgtt cgatcagctt
    16381 ttgcaaattg cggctgctgc tatcaagaat gctcaccacc tctttttgct ctggcgtaag
    16441 cggcccgaca acctgatcag ccagtaattc agtgccctcg cgcatactcg ccagtggcgt
    16501 ttttaattca tgagataaat gtcttaaaaa ttgatggcgt tgggattcca gccatgacag
    16561 gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt aactcgctcg gtccactgaa
    16621 cgagacgcta ttgcccagag aacgcccttc ccccagccgg ttgatcatgc gctcgatatt
    16681 tttcaccgga ccgataatca tcctcgtgaa aagcagtacc atcaccagac tcaccagaaa
    16741 tagcaccagc gattgccaac caaaatattg cccacgttcg gcgatttcac gctgaagttg
    16801 ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat tggcactggc
    16861 aaaggcttcc agacgcgcgg cggcagcagc atcgggaccg ctgttgttac actgaagttg
    16921 agccagacta ttcaaatcct gacgtaatgc ctggtagagt ttatcgtccg gcagcacgcc
    16981 tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg ctttgataaa ccttcgcaag
    17041 cgtagggtcg tccagcacgc agtactgacg atagctgcgc tccatctcca gtgccgcgtt
    17101 ggtcatcgct tcactgcgcc gggcatcaat aagcgtagtg cggttaacca gcgccgcctg
    17161 atcgctaagt gcattcaggc tttgccaggc ttgccatgcc agcaccaaca ggggcagcag
    17221 aatcagcaaa aatgccagca ttaccagttg tcgtaatgag cggggaaaaa cgggccagcg
    17281 tttcaaggcg ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg ggaagttcag
    17341 atacaacaaa gccgggaatt acccggcttt gttatggaat aaggcggtgc ctaactcgac
    17401 gtttcgcccg atggttgata tagctacgct gatatcagaa gttggacggc aggcaccttg
    17461 ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg gctgacataa gtcggcgaat
    17521 gagccactgc ttactattat gcagtaactg tgccaataaa gaaaatagtt tggtaacata
    17581 ctgattatac gctgctttga ggggtttatg gcttctccgc tatttgaatg taaatcagcc
    17641 actgt
//
LOCUS       DADTFG010000100        17604 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.100, whole genome shotgun sequence.
ACCESSION   DADTFG010000100 DADTFG010000000
VERSION     DADTFG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.100"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            104..7915
                     /locus_tag="J1138_003151"
                     /pseudo
     CDS             104..7915
                     /locus_tag="J1138_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            7963..8493
                     /locus_tag="J1138_003152"
     CDS             7963..8493
                     /locus_tag="J1138_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HBA8454917.1"
                     /translation="MSSVMKFSVVNAVVLLLLIVLTGVFYMKKSKEIAVVNIDAIYNQ
                     SAAGDMVRKHLNEVKSILEKGYEDAQKAFADNANKEALLLDARQKLERQYVIEENSAK
                     MVLSRAIDNAIGTWRKDNPKNIAVLSSGVVLAHNESADITKEVMALVEKENLVFNQLP
                     KVEVHNKNEEKPEQSK"
     gene            complement(8931..9005)
                     /locus_tag="J1138_003153"
     tRNA            complement(8931..9005)
                     /locus_tag="J1138_003153"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8970..8972),aa:Arg,seq:cct)
     gene            complement(9081..10013)
                     /locus_tag="J1138_003154"
     CDS             complement(9081..10013)
                     /locus_tag="J1138_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBA8454918.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNIIGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            10307..11062
                     /gene="mlaA"
                     /locus_tag="J1138_003155"
     CDS             10307..11062
                     /gene="mlaA"
                     /locus_tag="J1138_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBA8454919.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(11124..12464)
                     /gene="fadL"
                     /locus_tag="J1138_003156"
     CDS             complement(11124..12464)
                     /gene="fadL"
                     /locus_tag="J1138_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBA8454920.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSDLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            12836..13120
                     /locus_tag="J1138_003157"
     CDS             12836..13120
                     /locus_tag="J1138_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HBA8454921.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            13293..14603
                     /gene="fadI"
                     /locus_tag="J1138_003158"
     CDS             13293..14603
                     /gene="fadI"
                     /locus_tag="J1138_003158"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBA8454922.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     GDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            14603..16747
                     /gene="fadJ"
                     /locus_tag="J1138_003159"
     CDS             14603..16747
                     /gene="fadJ"
                     /locus_tag="J1138_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBA8454923.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYLLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            16950..17435
                     /gene="sixA"
                     /locus_tag="J1138_003160"
     CDS             16950..17435
                     /gene="sixA"
                     /locus_tag="J1138_003160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBA8454924.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         4570 a         3862 c         4738 g         4434 t
ORIGIN      
        1 agtcatttgg aaccatacaa cccaaaaatg ggatgttgtt tcagaattga cgagttgtag
       61 aaaaaaatgt aaatcaactc gcttaggaat tgctttatca gcaatggttc tggggggcgc
      121 tattgcaatt aattgcaata acgcaatggc agatgtcata ttgtcaccag actggcgtcc
      181 gggtacgaat aactcgggtg ttggggctgc aacagtaagt ggtaaaaccg aatacatcac
      241 tggtccaaat gtcgtccagt ctggtgggtc aggtcttatc tggatgactg tagaacaggc
      301 aattttaaat ggctacacca ctggagataa tttatccgga ttgatttacg tcaataccgg
      361 agaaaaaaca aaaaccatta cagtgaagga tgaggttact ggcgcttctc aaacacttca
      421 agtgtttgat actgactctt tctcacaacg cgatgcgggg actgggggaa atgaaaccat
      481 tcctggtttt agtggcactg cggatttttt caatgcgaca cgttttgtaa cagccaataa
      541 tggcggtaca gctattttgg atgtgggttc accagcaatc ggtaattttt ttaaaaatac
      601 acagcttgct gtagctgatg gagaaggttc ctctgttgta tggaactcgg tcaatgattt
      661 ttattttcag cctggtgcaa ccatgcaggg gggcggcgtt actcaaaaaa tcattgactc
      721 aatgaaatat gctggaacaa ttactgattg ggcgggaaaa gtacaccata ttaactctct
      781 tgatgattta aagcaatata atcaatattt gataaagtca ctagaggaca aaacgctttc
      841 ttataagcag tatgatgctg aatttaataa ggcccttatt gtcaccaagc ataattacaa
      901 tgtggatatg accgctgggg gacgtataga ctcaactcct tacaaagaaa atgtagggct
      961 gcttgctgtt ctccatgcaa ccaataacgc acgagcaata ttaggtaaaa cgggtaaatt
     1021 aactggagtt cttcctgcct atgggaatgg aggagggatc gttgcaacta atggtgggac
     1081 cggtgttaat gaaggggtca ttgatgccat tggtactgaa atgattgcct atcaagacag
     1141 taccattgtt aacgatggta cactttttgt ttgggataat aatgataaat atgctctcca
     1201 ggcagagggg atggttgccg gtagtaatgg ttcttcagcc attaataatg gtgttattaa
     1261 tattcgccca tttaaaaatg ctttcgctcc agaggggatt aacaccgcaa ttgttgttag
     1321 taatgggggc atggcaacaa ataaaggcac aataaatatt actgccgatg catcaactaa
     1381 tgataataat ggcaaaacac gaggtgtaaa tgttggagct ggaggctctt ttattaactc
     1441 ggccttcggg agcatcaatg taggtattgc ggaggataaa actgcgactc attcggctgt
     1501 gggttctgtt gcgattgaag tacaaaatgg tgcaaacaaa gtcgttaatg aaggtactat
     1561 ttttttgggc aggggggctc aggggaacta cggaatcctg gcaaaggatg ccgggactgt
     1621 tgatgtggta aataaaggga ctatcactat tgacggtcat gacagtgatg cacctgcact
     1681 gaatgttggc atgctggcaa ataatagctc cgggatgaaa aactccggga ttatcaatgt
     1741 taatggtctg aacagcaccg ggctacaggt aatcaatgca ggacagttga attctgacgg
     1801 tacgataaat gttggcggca agggtatcag tagtggtttc cgtaactatg gtgcctgggt
     1861 ggaaggtgcc ggaagcaatg ttaatgtatc cggaaaaatc agtcttgccg gtacgggggc
     1921 tgtgggggtt tttgctaaag atggcggcag tctgaccctg tcaggcaatg gtgcagtgct
     1981 atttggcagc agcgatcaga taggctttta tgtctatgga aaggactctg ccattcataa
     2041 taccggaagc ggtgttatgg atgtgtccac tgaaaactca acattattcc gtattgccag
     2101 tggtgcgaca ttccagggaa ctgcagatgc ttcttctgca cttacggcgt ctggtaagaa
     2161 ctcttatgca cttattgcca cggggaaatc ggatggcggt gtggcctcga cagtaacgtc
     2221 tggaggaatg accatcaacc tgacgggtga gggggctaca gcgactttaa ttgaaggggg
     2281 agcgcagggc acaattgaaa gtaatgccat tatcaatatg gataatgcca gtgcgatagc
     2341 cggtattgcg gatggcaatg gctatgatat ttccggcaaa ctcattaatc cgaaggacaa
     2401 gaccacacta ttaacggcgg gggctcagtt aagttccacc caggataaag tgaccgggta
     2461 tatcgcccgt aatggggcca ctctaaataa taccggtaat atcatcttta ctggaaagaa
     2521 tacagtgggc gtccgggttg aggagggggc tgttggtacc aacagcggaa atattacagt
     2581 tcaggatggt ggtgtgggac taattgctaa tgccacacaa gatgttacaa cgattaataa
     2641 ctcgggaaat ctcgttctca agggaggaga taatgctaac cgtacaacgg gtataaaagc
     2701 atctggtaca acaacaacgg ttaatatgac cgcgggtact atatctttgc agggacaggg
     2761 ggcgattggc gttgaggcca gcaataaagg gactgttaac cttgatggtt cggcagtacc
     2821 gaactttgct gctgacggct ctggtattac cgatcagatt gcttttcgta ttatcggaga
     2881 tggtgcaacc attaagacga atattgcacc gggaactctg ctggatgcca gtggggaacg
     2941 ttctgtactt ttccgtattg aagatggggc aaaacaggcc ggctctctgc tgatgaaaac
     3001 ctccgggaca ggcagccgtg gtatctgggc cacagggaaa gggagcaatg tcctggctga
     3061 tgctggcagt gatttccaga tcctgggcgc tcaggctcag ggattatatg taactggtgg
     3121 tgcgacagcg acgctgaaac agggggcatc agttaacctt gtaggggatg gcgctgttgt
     3181 cgcggaagtt gacggaaatg aatacgctct ggatggcagt attacacaaa cgaatactgg
     3241 ctcggttatt accaatgagg cagatatctc ttcgccgctg aataatgcca agggctttat
     3301 tacgcgtaat cagggactgt tgattaacaa cggcaacatt gatttcacta ccggtacaga
     3361 taatatcggc gtctgggttg ataacggccg ctttgaaaat acaggaagcc gtattgcggt
     3421 caatggcgtt gcattatttg ttgaaggtgc acagtctcag attaccagca caggagggga
     3481 tatcgtcgct gtggatggtg aggctgccat taagctgggg gcgggcgcgt cactgaacct
     3541 ggcagggagt ggcttgggta cgatcgaagg tcagaaaaat gcgcatggca tcctgctgga
     3601 tacaggggct gtggggctgg ttattgatgg tgcgaaaatc aatgttaatg ctgcaggtgc
     3661 ggtcggtcac gggattgaga accgcgcaga aattgaaggc attcagttaa ccaatacgac
     3721 tgaaattaat gtggctgatg gcattggtgt acgtacttct gcctccctgg ccaagaccaa
     3781 cagcggcact attaatgtag acggcagtgg aattgcactg gcgttccaga aagctgacgg
     3841 aagtgaaacc gataataacc tggatatgtc tgactccgct ggattggtca ttaacctgaa
     3901 gggtacggac ggcacgggta ttttcgccaa cactaaagat ggtgctgtcg tgaagagtgg
     3961 tgcaagtgtc aatgttatac aggccgatgg cggttccgct ctggtggtta acaatgcagc
     4021 cagtgaagtg gttcagagcg gtaatctcat ctctgcttct ctgagtcatg ccgtagtgga
     4081 tgcttcaaaa gcacaatcct ttaccaataa aggtcagatt aaagctgcgt ccaccaccgg
     4141 gactgcaatg gcgtttgatg acgccgtgaa taccaccgta ctgaatgaca gcggtgctga
     4201 aattcagggg gttgtggctc tgaacggcgg tgataacaca ttcaccaata aaggcagtat
     4261 taccggaacc gtcagtgcga aagagggtaa caatacattt ttatttgatg atggcagcac
     4321 actgacagga gaagtgactg caggaaatgg caataataat gtaacactca atggtaagac
     4381 tcatgttgat caggttactg ccggtaccgg gaagaacacc ttcaccatta aaggtgaagg
     4441 ggcaacctgg aacctgctgg atggcgggca gggagattct gattccctga tttttgataa
     4501 cgccattcat acgctggatt ctgttgtaaa actacagaat ttcgaacatg tcgggctgaa
     4561 gaacagttca cttgtcactc tgaaggaagc tcttgtgctg accgatgggg ggaacggtcc
     4621 gggttccgtc gatattgaat cgggcagcga actggccatt attcccgcag ttgcaggcaa
     4681 ctttaccttt gatccactgt taacaggcaa aggaacactg tctgcccgtc ttgatgccga
     4741 cacatctgct tttgaattca gccataacgt cggggatcaa tttgccggaa ctctgaagct
     4801 gggtactagt agctttgctc tggaagggct gaatacgagc gggttaaccc atgcaatgct
     4861 gatgtctgaa accgggaata tcacaacggt tggctccggt gttcagcaga ttggcggtct
     4921 tgggttcaat ggcggaacgc tgatttttgg ttccgttatg ccgggcgata ccattgccag
     4981 caacagtatt gaaacctctg ctgcaggtac gctggatatc cgggggaaag gcacaattca
     5041 ggtcaccatg ccagatgaag tgattaatga tattccggct gttgataccc gtaagaattt
     5101 gctggagcag gatgatgcgc agaccctggt cacgctggtg aatgcagcgg gtaccgtcac
     5161 cggtactggc gggcaactgc aactggtgga tgaaaacggg caggctattt ctcacagtca
     5221 gacgtttgat gtcactcagg gcggtgaagt tgtagctcag ggaaattatg actataagct
     5281 gctgggaagc tccgacggta ttaaaggtga cggactgtac ataggctatg ggctgaagtc
     5341 gctggattta cagggaaccg gtgataaagc gctggtgctg acaccgagag cgaatgccca
     5401 gggactgcag acagatcttg gcgcacagtt aacgggggca ggggatctgg ccatcgaagc
     5461 tgcggggcag gttgtcacac tgtctaacgg cggtaataac tacaccgggg atacgctggt
     5521 gcgcagcggc acattacaga tggcaaatga taatgtactt ggcgcaacag gtaatctgaa
     5581 cgtcgccagc aatgccgtct tcagaacaaa cggatacagc cagactgtcg gtgcactgca
     5641 gacagaaacg ggagcgcaca ttcagcttga tagcggtagt gtactaacgg tcagcggcac
     5701 gcagcgtcag ccgggggatg ataatggtgg catcattgaa aacaacgtgc tgtccggtga
     5761 gggaacgctg gctgtcacgg gcagcaatct gacggtacat ggcaccaata tcgggtttac
     5821 cggtaatgcc agccttaccc agggggcgct ggttgaaatg aacggcgcac aggggctggg
     5881 aagccagggc tccatcagct ttgaaagcct taatgatcgc ctggcgattg atatcgccga
     5941 cggtagcgga gtcagcagta acctgagtaa atcgctttcc ggagaaggtt cagtgggtat
     6001 actgaacacc actgatctga cgctttccgg ggataacagt aatttcagcg gtgaattccg
     6061 tgtgcagaaa gatgctgctc tgcgggcttc agatgaaaaa cacctgggca ccgggctgat
     6121 tgacagtgat ggtgtcacct ggcttaccgc cagtggaaac tggttactga agaatgatat
     6181 tactggctcc ggcgctctgg tgaaacaggg ggcgggtaat ctgatcatta atcatgagct
     6241 gacttatacc ggtgatacca cggttgagag tggcgttctg attgtcggtg atgattcagt
     6301 cacccgtgcc gctggcgcga cgttgtcagg atccaaaaac attcatgttc ttaatggcgg
     6361 cacactgagt gggctgggga ccgtttcggg tcaggttaat aaccagggaa cactggcatc
     6421 actgaatgct ctctcaggtt atgaaacggc agaggtgggc aattttaccg ttggctcact
     6481 gaccaatacc ggcgtcattc gtctggccgg tggtaagaca ggaaataccc tgaccgtaaa
     6541 tggcgattac accggtggcg gtacgttaat tatcaatacc gtgctggggg atgatacttc
     6601 taccactgac aaactgattg tcacgggtaa cacctccggt gataccggcg tggtggtgaa
     6661 taatgtccgg ggccaggggg ctcagaccgc tgacggcatt gagattgtgc atgtcggtgg
     6721 gcagtctgac ggtaacttca gactgcagaa ccgtgcggtg gcaggggcgt gggaatattt
     6781 cctgcataaa ggtaatgcgg gtggtactga cggtaactgg tatctgcgtt ctgaattgcc
     6841 gccagaacca caaccgcagc cgcagccaca accgcagccg cagccacaac cgcagccaca
     6901 gccacacccc acgccggata aacctgtaca gaaggtctat cgtccggaag cgggctctta
     6961 tattgcgaac atagcggcag ccaacacgct gttcaacatc agaatgcacg atcgtgaggg
     7021 ggaaacttac tataccgatg tgtttactgg tgagaagaaa gcaacaagta tgtggatgcg
     7081 tcatatcggc ggtcataatc gctggaagga cagcagttca cagctgaata cgcaaagtaa
     7141 ccgttatgtt gtgcaactgg gtggcagcat tgcgcaatgg actgacggtc aggaccgtct
     7201 gcaactgggg attatggccg gttatggaaa tgagaagagt tccacgacat cctcgctttc
     7261 gggatataaa tctaaaggtg cgatcaatgg atacagcacc ggtttatacg gaacgtggca
     7321 gcagaatgac ggtaatgaca acggggctta tgtcgatacc tggatccagt acggatggtt
     7381 taataatacc gttaatggtg aaaaactggc tgcggagtcc tggaaatccc gtggatttac
     7441 cggttcagtg gaagcgggtt atacctttaa agccggcgaa tttaccggta gccagggtag
     7501 tcactatgac tggtatattc agccgcagtc ccagatcacg tggatgaatg taagagccag
     7561 tgagcatacg gagaagaatg gcacgaaagt tcagctcagc ggagacggta atatccagag
     7621 tcgtctgggc gtcagaactt acctgaaagg taagagtgcg tctgatgata ataaggcgca
     7681 ttagtttgag ccatttgtgg aagttaactg gatccataat acacggagct ggggtgtcaa
     7741 aatggataat acggcactca gtcaggacgg agctacaaat attgctgaag tcaaaacggg
     7801 tgttcagggt aaattgtcag ataacctgaa tgtctggggc aatgttggtg ttcaggctgg
     7861 cgataaaggg tacagtgatg cccaggcaat gctgggtatt aaatatatat tctaatcaca
     7921 cagtggcagg aggggacctc ctgccacctc agggttgtca taatgagttc tgtaatgaaa
     7981 ttttctgttg ttaatgcagt agtgctgctg ttgctgattg tacttactgg tgttttttat
     8041 atgaaaaagt caaaagaaat tgcagttgtt aatatcgatg ctatttataa tcagtctgcc
     8101 gccggagaca tggtcagaaa gcatctgaat gaggttaaga gtattcttga aaaaggctat
     8161 gaggatgcgc aaaaagcatt cgcagataac gcgaataaag aggcgctgct tcttgatgcc
     8221 agacaaaaac tggagcgaca atatgttatt gaggaaaata gcgcaaaaat ggtgttatcc
     8281 cgtgcgatag ataatgccat aggtacatgg agaaaagaca atcctaaaaa tattgctgta
     8341 ttgtcttcag gtgttgtgct tgcgcataac gagtcagctg atatcaccaa agaagtcatg
     8401 gctctggttg aaaaagaaaa tctggttttt aatcagttac ccaaagtgga agtgcacaat
     8461 aagaatgaag agaaaccaga acaaagtaag taatcactag attctgacga tttccagaag
     8521 ggagattcag caggccagca tctgtaatca ttcagatttg ctggcttgct gctaaatatg
     8581 gttgctaaga aaaatagcag gccagtaatt tattcagatt actgaatata ataatttttc
     8641 aggaacggtt atgctgaagg tagtatgttt aatcgacaca attatttgct gtgtctaata
     8701 tgaggtgaca tttttatggt cactgaccag caagactgac ccgtattgat tagcctgatt
     8761 attggcaact tattggggta gtcattttga catcgaatgg gggaggtttc cagttaagtt
     8821 cacgaatgca tgatgatttg cgttcacata tcgaagtact ggttgactat actaaggatt
     8881 ttaagataac tcactgattg taagtgggtt attgcgaggg ggatgataga tggtgtcccc
     8941 tgcaggaatc gaacctgcaa ttagccctta ggaggggctc gttatatcca tttaactaag
     9001 aggacaatgc ggcatgagta tacccgctaa tggactacgg ggtaagtaca cagctgctcg
     9061 attgcttaaa ccctcgccat ttatgccggg tttttatcat ttttcttaat gttttccgca
     9121 cgttctgctt tttgacgtgc ttctgctttg cgcttgttgc tcatgtcgtt acgaatctgt
     9181 gcatgactca ttaacgcaaa gataaaggtg ccgccgcaga tgttccccgc taaagtaggt
     9241 agtgcaaagg gccagatgaa atcgctccag tgcagcgtgc cgttaaacac cagatagagg
     9301 atttcaacag aaccgacaac gatgtgggtg gtgtcaccca gggcaataag ccaggtcatc
     9361 aatataatca ccacaatctt tgccgcaccc gctgcaggaa acatccatac catagtggcg
     9421 atcagccagc cggaaatgat cgcgttggca aacatctcgc tgggggtgtt cttcatcaca
     9481 tccatgccga ttttgacaaa tgcatcgcga gtttcttcat tgaagatagg catatattca
     9541 aatgcccacg cagcaatacc tgtcccgata atattaccca gcagcacgat gccccataac
     9601 cgcataagta agccgacgtt gctcattgtc ggtttttgca tgacgggtag taccgcagtt
     9661 acggtgtttt cggtaaataa ttgctggcgg gccattatga cgataataaa accaaaggta
     9721 taaccgagat tctccagcaa gaagctgccc ggcacgcctt ccagttcgac atgaaatatc
     9781 ccttttgcca gtagcgaagc gcccatcgac aggcccgccg caatggctga ccacagtagc
     9841 gccattgcgt cgcgttccag ctctttttca ccatcctggc ggatatgctc atgaattgcc
     9901 atcgcccggg aggggagtcg gtcttcatct atttctattt ttttaccgcg ctctttttct
     9961 tcgctctcaa cttcaatttc gtcgctgtgt tgatcaattt tgtcgttgtc catggtctct
    10021 tctcttgaat tagcacgtat agctaaagcg tagcggcttt tttgctcgca actggcggga
    10081 gttactctga aaatgtagaa aaggccgcgt ttgccttttt ctgtttctat tgaatcaagt
    10141 agcctacagg gcggcgatta ccaggctatg atcaaatcag caaatcaggg cgtctggaca
    10201 tcagttgacg tgctgttaca atcgcccaca cctaaacagg cggatacggt atcgttccgt
    10261 catggatggc aaactgcata agccataaaa aaacagggag acatttatga agcttcgcct
    10321 gtcggcgctt gctctgggaa ctacgcttct ggtggggtgt gcgagttccg gtacagatca
    10381 gcaagggcgt tctgacccgt tagaagggtt taaccgcact atgtacaact tcaacttcaa
    10441 tgtattagac ccgtatattg ttcgaccggt cgctgtcgcc tggcgtgatt atgttccgca
    10501 acctgcgcgt aacggtttga gcaactttac cggcaacctt gaagaacctg cggtgatggt
    10561 taactacttc ttgcagggcg acccttatca ggggatggtc cactttaccc gctttttcct
    10621 gaataccatt ttgggaatgg gcggctttat tgatgttgca gggatggcga acccgaaact
    10681 gcaacggact gaacctcacc gcttcggtag tacgcttggt cattatggtg tgggttatgg
    10741 gccgtacgtt cagttaccgt tctacggtag cttcacgctg cgtgatgacg gtggtgatat
    10801 ggcggatagt ctttacccgg ttctttcctg gctgacctgg ccgatgtctg tgggtaaatg
    10861 gacgcttgaa gggatagaaa ctcgtgcgca gttgttggat tccgacggtc tgctgcgtca
    10921 gtcctccgat ccttatatta tggtgcgtga agcgtacttc cagcgtcatg atttcatcgc
    10981 taatggcggt gaactcaaac cgcaggaaaa tccgaacgcg caagcgattc aggatgattt
    11041 aaaagatatc gattctgaat aagaaacaaa caaaaaaggt gagtcgcaaa actcaccttt
    11101 tttcttatta agctaacgcg ttatcagaac gcgtagttaa agttagtacc gaacagccag
    11161 gctttacctt cagactcgaa ggaataagct gccgctcctt caccttcggt gaatttcact
    11221 ttctgaccat gcatataaga tgcgccaaca tcaatggatg catcttcatt aaatgcgtag
    11281 gttaacccag cacttaacca caaacgatcc tggtcaggaa tggaaataga acgtttatct
    11341 gccggaaccg ggctgtcgtc ataagcgata ccggtacgga aggtccagtt tttatcataa
    11401 tagtaggttg tacccaacgc gatacgatac gcgtcccgga atccttcatc tttatagaac
    11461 agagtctgac cactatcgcc tttcgctttc aattcctgga actggctcca actggtatag
    11521 gccatgctgt agtgaattgc ccactgcggc gcaacacggt tataaccaga aatctcccac
    11581 atctcaggca ggttcagcgt taaggatcca ttttgggttg cccccatcgt gccatacggt
    11641 aagcccatgt tacccaaaat attgttcaga tttgggttaa tggagctttt gtaatcccca
    11701 tcgaagtcga ttttaacttc tgaacgatat gtcagccccc aacggttatc tttatccagt
    11761 tcataaagaa tacccgcgtt ccagccaaag ccccattcgt cacctttcaa atgagcaatt
    11821 tgagagtccg gagtattaat accattggtt aatgaagcgg gtacaccctg ggcaatcaac
    11881 tgttgagaca acaatggccc cagcgaaccg gcgtaacgct caatttttgc cttggcataa
    11941 actgcatcaa aaccaagacc aaaactccag ctattgttaa ggcgataagc cccgctaagg
    12001 ttaaggttga ttgtttgcag atcggtttta ccgccataga taccagcagc gtagttgtcg
    12061 gtaaattccg tagccaggcc atagttggaa gttattgacg ctccccaacc aaactgatcg
    12121 ttaataggcg caacaaagtg caggttaggt acccatgctg tcggtgcgat atttttagca
    12181 tttgcactct gaccggttaa atcagaatgt ccggtaatat ctacatccgg atcaatataa
    12241 accgcacccg cagaaaatgt cgggcggtca aacatggtaa tcaatgcggg gttacggcta
    12301 acgttacctg catcatcggc aattgcacct tcccctgaat aagcccggcc caggccagag
    12361 gaagaaaatt cgtttaactg aaagcctgcc gaccaggcct gggtggagat aagtgccact
    12421 gcgactgcga gagcagactt tgtaaacagg gttttctggc tcatgaccat aacctcaatg
    12481 atttattttt atacaaacta tgttacgtgc tgtaacagga gcgcgaagtg tagggtctga
    12541 ggtacatctt agaaatcaga ccagtggcga gagtataggt cggaccagct ggaatgttgc
    12601 aagtatgttt ctatcttttt tcagatatga tctaagaaac gcgatccagc tggcaaaatt
    12661 gggaggtggc gcactcatgg attcgggtga attttgtttt agatcatttt taagtgtgat
    12721 ttcggtcact tatccgattt tgcagaatta acaactggag cagcactttc cggaagcgta
    12781 aaatatccac aacgtttatc tggcaccttg ggtgcacata cagaggaaat caacgatgag
    12841 taaatgtagt gctgatgaaa ccccggtttg ctgctgtatg gatgttggca ccattatgga
    12901 caactccgac tgcaccgcgt cttacagccg tgtgtttgca aaccgcgcag aagcagaaca
    12961 aacgctggcg gcgctgactg aaaaagcccg tagcgtggaa tccgaaccgt gcaaaatcac
    13021 cccgactttc actgaagagt ctgacggtgt gcgcctggat atcgatttca cttttgcctg
    13081 cgaagctgaa atgctgatct tccagctggg tctgcgttaa ttcctctcgg gtacatacgc
    13141 ccctgtcagt tgctggcagg ggcgttttct tttctcttct tcttttctct ctgtgtcata
    13201 gctcccactt tcccttttct ccacttggct aaatgtaaaa aaatggttaa gactatgatc
    13261 aggtcagacc actttattta caggggagtg ttatgggtca ggttttaccg ctggttaccc
    13321 gccagggcga tcgtatcgcc attgttagcg gtttacgtac gccttttgcc cgacaggcga
    13381 cggcttttca tggcattccc gcggttgatt tagggaagat ggtggtaggc gaactgctgg
    13441 cacgcaccga gatccccgct gaagtgattg aacaactggt cttcggtcag gtcgttcaga
    13501 tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg tactggaatg aatgtgcata
    13561 ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt ccaggcggtt gcaaacgtcg
    13621 cagaaagcct gatggcggga actattcgag cggggattgc cggtggggca gattcctctt
    13681 ccgtattgcc aattggcgtc agtaaaaaac tggcgcgcgt actggttgat gtcaacaaag
    13741 cccgcacaat gagccagcgc ctgaaacttt tttctcgctt acgtttgcgt gacttaatgc
    13801 cggtgcctcc ggcagtggcg gaatactcca ctggattacg gatgggggac accgcagagc
    13861 aaatggcgaa aacctacggc atcacccgag aacagcaaga tgcactggcg caccgttcgc
    13921 atcagcgtgc cgctcaggcg tggtcagaag gtaaactcaa agaagaggtg atgactgcct
    13981 ttatcccccc ttataaacaa ccgcttgtcg aagacaacaa tattcgcggt aattcctctc
    14041 ttgctgatta cgcaaagctg cgcccggcgt ttgatcgtaa acacgggacg gtaacggcgg
    14101 cgaacagtac gccgctgacc gatggcgcgg cagcggtgat cctgatgact gaatcccggg
    14161 cgaaagaatt agggctggta ccgctggggt atctgcgcag ctacgcattt actgcgatag
    14221 atgtctggca ggacatgttg ctcggtccag cctggtcaac accgctggcg ctggaacgtg
    14281 ccggattgac gatgggcgat ctgacattga tcgatatgca cgaagccttt gccgcccaga
    14341 cgctggccaa tattcagttg ctgggtagcg aacgttttgc tcgcgacgta ctggggcgtg
    14401 cacatgccac tggcgaagtg gacgatagca aatttaacgt gcttggcggt tcgattgctt
    14461 atggacatcc cttcgcagcg accggcgcgc ggatgattac ccagacattg catgaacttc
    14521 gccgtcgcgg cggtggattt ggtttagtga ccgcctgtgc tgcgggtggg cttggcgcag
    14581 caatggttct ggaggcggaa taatggaaat ggcatcagcg tttactctta atgttcgtct
    14641 ggacaacatt gctatcatca ccatcgacgt accgggtgag aaaatgaata ccctgaaggc
    14701 ggaatttgcc tcgcaggtac gcgccattat taagcaactc cgtgaaaaca aagagttgcg
    14761 tggcgtggtg tttgtctccg cgaaaccaga caacttcatt gctggcgcag acatcaacat
    14821 gatcggcaac tgcaaaacgg cgcaagaagc ggaagtactg gcgcggcagg ggcaacagtt
    14881 aatggcggag attcatgctt tgcccattcc ggttatcgcg gctattcatg gcgcttgcct
    14941 gggcggcggg ctggagttgg cgctggcgtg ccacggtcgt gtttgtactg acgatcctaa
    15001 aacggtgctc ggtttgcctg aagtacaact tggattgtta cccggttcag gcggtaccca
    15061 gcgtttaccg cgcctgatag gtgtcagcac agcattagag atgatcctca ccggaaaaca
    15121 acttcgggcg aaacaggcag taaagctggg gttggtggat gacgtcgttc cgcactccat
    15181 attgctggaa gccgctgttg agctggcaaa gcaggatcgc ccatcttcac gccctctacc
    15241 tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg ctgctgttca aaatggtcgg
    15301 taagaaaaca gaacacaaaa cgcaaggtaa ctatccggcg acagaacgca tcctggaggt
    15361 tgtcgaaacg ggattagcgc agggcaccag cagcggctat gacgctgaag cacgggcgtt
    15421 tggcgaactg gcgatgacgc cgcaatcgca ggcgctgcgc aatatctttt ttgccagtac
    15481 ggaagtgaaa aaagatcccg gcagtgatgc gccgcctgcg ccattaaaca gcgtggggat
    15541 tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc actgcttgta aagcggggct
    15601 tccggtcaga attaaagata tcaacccgca gggcataaat catgcgctga agtacagttg
    15661 ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa gccagcgaac gtgacaaaca
    15721 gctggcatta atctccggaa cgacggacta ttgcggcttt gcccatcgcg atctgattat
    15781 tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg gtggcggaag tggagcaaaa
    15841 ttgcgctact cataccatct ttgcttcgaa cacgtcatct ttaccgattg gcgatatcgc
    15901 cgctcacgct gcgcgacctg agcaagttat tggcctgcat ttcttcagtc cggtggaaaa
    15961 aatgccgctg gtggagatta ttcctcatgc gagtacatcg gcgcaaacca tcgccaccac
    16021 ggtaaaactg gcgaaaaaac agggcaaaac gccaattgtc gtgcgtgaca aagccgggtt
    16081 ttacgtcaat cgcatcttag cgccttacat caatgaagct atccgcatgt tgaccgaagg
    16141 cgagcgtatt gagcatatcg ataccgcgct agtgaaattt gggtttccgg taggcccaat
    16201 ccaacttttg gatgaggtag gaatcgacac cgggactaaa attatgcctg tactggaagc
    16261 tgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt tcttcaattt tgaacgacga
    16321 tcgcaaaggc agaaaaaatg gccggggttt ctatctttat ggtcagaaag ggcgtaaaag
    16381 caaaaaacag gtcgatcccg ccatttacct gctcattggc gcacaagggc aggggcgact
    16441 ctccgcaccg caggttgctg aacggtgtgt gatgttgatg ctgaatgaag cagtacgttg
    16501 tctggacgag caggttatcc gtagcgtgcg tgacggggat ataggcgcgg tatttggcat
    16561 tggttttccg ccatttctcg gtggaccgtt ccgctatatc gattctctcg gcgcaggcga
    16621 agtggttgca ataatgcaac gactggccac gcagtatggt tcccgtttta ccccttgcga
    16681 tcgtttagtt gagatgagcg agcgtgggga aagtttttgg aaaacaactg caactgacct
    16741 gcaataagaa ggtcaaagtt atatgaatcc gcgctgaatg gcggagtttt gctcaaaatg
    16801 taaacgcata ttgactatac ttacgccatt gaggtaaaaa acagcgtttc attcggtgaa
    16861 tggataaggc acaatgccgg ccaccgtttt ctttctctgg tttcagatga aagaaaacgg
    16921 gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt tatcatgcgt cacggcgacg
    16981 cagccctcga tgccgccagt gattcggttc gtcctctgac cactaacggt tgtgacgaat
    17041 ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga aatcgaacgc gttctggtga
    17101 gcccgttcct gcgagccgag caaacactgg aagaagtagg ggattgtctg aacctgcctt
    17161 ccagtgcaga agttctgccg gagttaacgc cctgcggcga tgttggtctg gtcggtgcct
    17221 atttgcaggc gttgaccaat gaaggtgttg cttcggtact ggtgatctcc cacttaccgt
    17281 tagttgggta tctggttgcc gagttatgtc cgggcgaaac gccgcctatg tttaccactt
    17341 cagcgattgc cagcgtaacg cttgatgaaa gcggaaaagg ccaattcaac tggcaaatga
    17401 gcccttgtaa tttgaagatg gcaaaagcaa tttaagcaat caatgcccgg ttacgtgcgc
    17461 gtataccggg cagtttctcc gtcatacaat cattcttttt cttgtctaaa tgttgttaat
    17521 atttaacaac tctcgatagt tattatattt cccattattg aatcccgtaa taaaataaag
    17581 aagataatgg ttttgtgtct tgaa
//
LOCUS       DADTFG010000101        17586 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.101, whole genome shotgun sequence.
ACCESSION   DADTFG010000101 DADTFG010000000
VERSION     DADTFG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.101"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            341..1129
                     /gene="fabI"
                     /locus_tag="J1138_003161"
     CDS             341..1129
                     /gene="fabI"
                     /locus_tag="J1138_003161"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBA8454925.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1274..2401
                     /locus_tag="J1138_003162"
     CDS             1274..2401
                     /locus_tag="J1138_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBA8454926.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINQWADRDSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            2469..4403
                     /gene="rnb"
                     /locus_tag="J1138_003163"
     CDS             2469..4403
                     /gene="rnb"
                     /locus_tag="J1138_003163"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBA8454927.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(4480..4583)
                     /locus_tag="J1138_003164"
                     /pseudo
     CDS             complement(4480..4583)
                     /locus_tag="J1138_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4637..6622
                     /gene="pdeR"
                     /locus_tag="J1138_003165"
     CDS             4637..6622
                     /gene="pdeR"
                     /locus_tag="J1138_003165"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBA8454928.1"
                     /translation="MKTVRESATLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            6770..6943
                     /locus_tag="J1138_003166"
     CDS             6770..6943
                     /locus_tag="J1138_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454929.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            7032..7781
                     /locus_tag="J1138_003167"
     CDS             7032..7781
                     /locus_tag="J1138_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8454930.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNATIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            8050..8268
                     /gene="osmB"
                     /locus_tag="J1138_003168"
     CDS             8050..8268
                     /gene="osmB"
                     /locus_tag="J1138_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBA8454931.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8394..8720)
                     /gene="yciH"
                     /locus_tag="J1138_003169"
     CDS             complement(8394..8720)
                     /gene="yciH"
                     /locus_tag="J1138_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBA8454932.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
     gene            complement(8720..9457)
                     /gene="pyrF"
                     /locus_tag="J1138_003170"
     CDS             complement(8720..9457)
                     /gene="pyrF"
                     /locus_tag="J1138_003170"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBA8454933.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVSELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREVLVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(9648..10817)
                     /gene="lapB"
                     /locus_tag="J1138_003171"
     CDS             complement(9648..10817)
                     /gene="lapB"
                     /locus_tag="J1138_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBA8454934.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(10824..11132)
                     /gene="lapA"
                     /locus_tag="J1138_003172"
     CDS             complement(10824..11132)
                     /gene="lapA"
                     /locus_tag="J1138_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBA8454935.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            complement(11281..12045)
                     /gene="pgpB"
                     /locus_tag="J1138_003173"
     CDS             complement(11281..12045)
                     /gene="pgpB"
                     /locus_tag="J1138_003173"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBA8454936.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVMWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            12215..12805
                     /gene="ribA"
                     /locus_tag="J1138_003174"
     CDS             12215..12805
                     /gene="ribA"
                     /locus_tag="J1138_003174"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBA8454937.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(12869..15544)
                     /gene="acnA"
                     /locus_tag="J1138_003175"
     CDS             complement(12869..15544)
                     /gene="acnA"
                     /locus_tag="J1138_003175"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBA8454938.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGESVYLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(15694..15789)
                     /locus_tag="J1138_003176"
     CDS             complement(15694..15789)
                     /locus_tag="J1138_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454939.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(15903..16070)
                     /locus_tag="J1138_003177"
     CDS             complement(15903..16070)
                     /locus_tag="J1138_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454940.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(16073..16237)
                     /locus_tag="J1138_003178"
     CDS             complement(16073..16237)
                     /locus_tag="J1138_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBA8454941.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVV
                     VALFIWKLWG"
     gene            complement(16532..17506)
                     /gene="cysB"
                     /locus_tag="J1138_003179"
     CDS             complement(16532..17506)
                     /gene="cysB"
                     /locus_tag="J1138_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBA8454942.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4481 a         4508 c         4165 g         4432 t
ORIGIN      
        1 gtgaagatag ttgacaatat taagttggag tcaattcatc agaaagacag cgacttgttg
       61 tcgccagttc tgttttcatc atcaaactta tcggagctaa tacgaatagt ttcaaagtca
      121 gattctataa gatatttccc caacctcccc cttgcaggcg tcaggagtag ataacgcaaa
      181 atgctctgta ccgcagcttc tctccgctac agaaagcgca actatagcca cccacagcca
      241 ggttgattat aataaccgtt tatctgttcg tactgtttac taaaacgacg aatcgcctga
      301 ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc tttccggtaa
      361 gcgcattctg gtaaccggtg ttgccagcaa actgtccatc gcctacggta tcgctcaggc
      421 gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac tgaaaggccg
      481 cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcagtgcg atgttgccga
      541 agataccagc atcgacacca tgttcgctga actggggaaa gtttggccga aatttgacgg
      601 tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact atgttaacgc
      661 cgttactcgt gaaggcttca aaattgccca cgacatcagc tcctacagct tcgttgcaat
      721 ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc tttcctacct
      781 tggcgctgag cgcgctatcc cgaattacaa cgttatgggt ctggcaaaag cgtctctgga
      841 agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat
      901 ctctgctggt ccgatccgta ctctggcggc ttccggtatc aaagacttcc gcaaaatgct
      961 ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt actattgaag atgtgggtaa
     1021 ctccgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag tggtccacgt
     1081 tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa ctgaaataat cgttctgttg
     1141 gtaaatattg ggcggcgttc tgccgcccgt tatctctgtt atacctttct gatatttgtt
     1201 atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt tcctttacga
     1261 ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt atcatgaaac
     1321 gcaatccagt actgcggagt atgacgttct gcctctggtc ccggaagccg caaaggtcag
     1381 cgatcccttt ctgctcgacg tgatccttga tgaagaaacg ctggcaccct tcctttcatg
     1441 gctggtccct gcgcgcgttc ttgcagtaga gttgttccct gaccagctta ccgtgacccg
     1501 ttcacagacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg ctcaggtttg
     1561 cggcgtccaa cggttatgta actactattc ggcgcgactt acgccgctcc ccgggcctga
     1621 ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc gccaactggc
     1681 tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg ttggtcttac
     1741 tgtctgtgac tgtgtaatca ttaaccaaat cattggtttt attggctttc aggcgcggac
     1801 aattgcgaca tttcaggctt atctcgggca tccagtacgc tggttacccg ggctggagat
     1861 acaaaactac gccgacgcgt cactgtttgc tgatgaatca ttacgctggc gaagcagcta
     1921 tgaggtggaa aaactccctg aagagcacac aaaaagttca actgcagaac tttgccaact
     1981 ggccaacaca ctctctctcc accctatttc actttccctt ctcgaaaagt tgttaaacag
     2041 cacacgggtc aatacacagc cggataatca gcttgcggcg ttgttatgcg cgcggataaa
     2101 tggcagtcct gcttgttttg ccgcctgtat gaattcatca aatgaatata aaaaaatcag
     2161 cccccttctg cgcaaaggcg aaaatgaaat taaccaatgg gctgaccgtg attctgttga
     2221 gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct ttagcgccgc
     2281 ccagttcagc ccattactcg aacacgaaaa atcatcaacg cagattatta atctgctggt
     2341 atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg agacatatta
     2401 accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg ccaccaaaat
     2461 agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa ctgcattccc
     2521 agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc ttcctggaag
     2581 tcgacgcgca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc atgcatggcg
     2641 accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag ccagaagaac
     2701 tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat gaccgtctgg
     2761 ccatcgttcc tgatcatcca ctcttaaaag acgccattcc ttgccgcgca gcccgtggcc
     2821 tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt catccgctga
     2881 aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt gacgatcact
     2941 ttgtaccgtg gtgggttacc cttgcgcgtc ataatctgga aaaagaagca ccagacggcg
     3001 tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgactgcg ctggattttg
     3061 tcaccatcga cagtgccagc acagaagata tggatgacgc ccttttcgcc aaggcgttac
     3121 cggatggcaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg attgctgaag
     3181 gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg cctggcttca
     3241 acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc gccaatgaag
     3301 tccgcccggt actggcatgc cgcatgacgc tctccactga tggcaccatt gaagataata
     3361 tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac caggtttctg
     3421 actggctgga aaataccggt gactggcagc ctgaaagcga agcaattgcc gaacaagtcc
     3481 gtttgctagc gcaaatatgc caacgccgcg gcgagtggcg tcataaccac gcactggtgt
     3541 ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg ctggatatcg
     3601 tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt gccgctaaca
     3661 tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac gtgcatatgg
     3721 gctttgatcc ggcgaatgcc gacgcgttgg cagcgttgct gaaaacgcac ggtctgcatg
     3781 tcgatgctga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt gaactggatg
     3841 cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt gctgaaatta
     3901 gcactgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc tggacttcgc
     3961 cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt atcaaaggcg
     4021 aaactgcgac gcgtccacag gatgaaatca ctgtccaaat ggccgagcgt cgccgtctca
     4081 accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg aaagacaaag
     4141 ccgggaccga cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc ggcatgcgtg
     4201 ttcgtttggt tgataacggc gctatcgcct ttattccagc acctttctta cacgctgtgc
     4261 gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt gaaacggctt
     4321 acaaagtgac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa acccgcagca
     4381 ttattgcgcg cccagtcgcg taatctcctt tcacggccca ttcctcatgg atgggccgtt
     4441 tatttccccg ctcaaccttc ctcatatccc ggcagatttt taactgactt tcgtttgaaa
     4501 actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat gtgtttagcc
     4561 gccgttaatt tcatgtaagt catgttgttc cttttattta actgcggact ccgctgttaa
     4621 ccggaggata tgcatcatga aaaccgttag ggagtccgca acgttgtaca actttctcgg
     4681 atcgcacaat ccatactggc ggttgacgga aagcagcgat gttttgcgct tttctaccac
     4741 cgaaatcaca gaacccgatc gtattttgca actatctgcc gaacaggctg ctcgcatcag
     4801 ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg aaagcgatct
     4861 ttctgtgcat ctggtaggac gaaaaatcaa taaacgagaa tggggaggca acgcgtctgc
     4921 atggcatgac acaccggcgg ttgctcgtga tttatcacat gggctttcct ttgctgagca
     4981 ggtagtttct gaagcacatt ccgcaatagt gattctcgac agccggggga atatccagcg
     5041 cttcaatcgg ttatgtgaag attacacagg gttgaaagaa cacgacgtca ttgggcaaag
     5101 cgtgtttaaa ctgtttatga gtcgtcgtga agctgcggca tccaggcgca ataaccgtgt
     5161 attttttcga agcggcaatg catatgaagt cgaactatgg ataccaacac gtaaaggcca
     5221 gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa acgagatttt
     5281 tttaatctgt tccggcaccg acattaccga agagcgccgc gctcaggagc gactgcgtat
     5341 tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcgatgc aggagttaat
     5401 cgatcacgct attaatcagg cagataacaa taaagttggg gttgtgtatc ttgatttgga
     5461 taattttaaa aaggtcaacg acgcctatgg gcatttgttt ggtgaccagt tattacgcga
     5521 cgtgtcattg gctattttaa gctgtctcga acatgaccag gtattggcgc gtccaggtgg
     5581 cgatgagttt ctggtactgg catccaatac ctcacaaagc gcgctggaag cattggcatc
     5641 acgaattttg acccgcttac gactcccctt tcgcattggt ttaattgaag tttataccag
     5701 ctgttcagta ggtatttcac tctctcccga acatggttcg gacagcgcgg ctattattcg
     5761 tcacgccgac acagcaatgt acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt
     5821 taccccagaa atgaatcagc gggtatttga atatctctgg ctggatacca acttgcgtaa
     5881 agcactggaa aacgatcagt tggttattca ctatcaaccg aaaatcacct ggcgtggcga
     5941 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
     6001 gttggacttc atttcctacg ccgaagaatc agggctaatt gtgcctttag gccgttgggt
     6061 gattctcgat gtcgtacgcc aggtagcgaa gtggcgggat aaaggcatta acctgcgagt
     6121 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
     6181 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cggagagctg
     6241 cctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc gattaggtgc
     6301 gcaagttcat ctggacgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
     6361 tccgatcgat gccatcaaac ttgaccaggt ttttgttcgg gatattcaca aacagcctgt
     6421 ctcgcaatca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
     6481 cgccgaaggt gtagaaagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
     6541 gcgacaagga tttttgtttg ccaaaccgat gcccgccgtt gccttcgaac gctggtataa
     6601 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat attccgtcat
     6661 tatatatcgt attcccggat gataattagc cgttgctgtt aaacacttct cttttagcgg
     6721 caatttttgc attattcatt tttccccttt tgctataagg atgtccttga tgtctgaatt
     6781 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
     6841 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
     6901 tgaatctacg ccagacatcc cgaaggcacc gtgggagatc taacaatcgt gccttcaggt
     6961 tttcctattt gaggatgccc ggcgcgtcag tattttttat ttagtattat aacgttataa
     7021 gaattacagc gatgaattcc cgacaacaaa ctattctaca gatggtcatt gaccagggtc
     7081 aggttagcgt aaccgatctg gcaaaagcca ctggagtttc tgaagttacc attcgccagg
     7141 atctcaacac cctcgaaaaa ctgagttacc tccgacgtgc acatggtttt gcagtttcgc
     7201 ttgatagtga tgacgtcgaa acccgtatga tgagcaacta tacgctgaag cgtgaactcg
     7261 ccgagtttgc cgcgtcactg gttcaaccgg gcgaaaccat ctttatcgaa aatggtagca
     7321 gcaatgccct gctcgccagg actctgggcg agcagaagaa aaatgccact atcatcacgg
     7381 tcagcagcta catcgcgcat ttgctgaaag acgcgccttg tgaagtcatt ttgctcggtg
     7441 gcgtgtacca gaaaaaaagc gaaagtatgg ttggcccttt gacacgccag tgcatccaac
     7501 aggtgcattt cagcaaagca tttattggta ttgatggctg gcaacctgaa actgggttta
     7561 ccggtcgcga tatgatgcgt accgatgtgg tcaatgccgt gctggaaaaa gagtgcgagg
     7621 cgatagtcct gaccgacagc tcgaaatttg gtgctgtaca ttcatactcc atcggccccg
     7681 ttgagcgatt caatcgcgtg attaccgatt cgaaaatacg cgccagcgat ctgatgcatc
     7741 ttgagcacag taaactcacc gttcacgtcg ttgacattta accatttccc ttctctacgg
     7801 atgatttgca gtttggcaaa tcatccgctc taagatgatt cctggttgat aattaagact
     7861 atttacctgt tattaacact ctcaagatat aaaattatta tcagcgatat aacaggaagt
     7921 cattatcacc tgcgtgatat aaccctgcgc gcgagcggat ttcacggaat aatttcacct
     7981 ggcttattct tagctattat agttatagag agcttacttc cgtgaatcat aaattcagga
     8041 gagagtatta tgtttgtaac gagcaaaaaa atgaccgcgg ctgttctggc aattaccctg
     8101 gcaatgtctc tgagtgcctg ttctaactgg tctaaacggg accgcaacac cgcaatcggt
     8161 gcaggtgcag gggcattagg cggtgcagta ctgaccgatg gcagtacgtt gggtacatta
     8221 ggtggtgcag ccgtcggtgg tgttattggt caccaggtcg gtaaataatc gtaaataaat
     8281 gtcgattatc gactcgcaat tataatacgc ttgattctgt tcagtacgaa ataactgaat
     8341 aatacacaca gtaaaatatt cgaaagccac ggatatatcc gtggcttttt atgttagccg
     8401 cctgcgagtt ttaccttcat ccctttcgct tcaagcattg attttaataa atcacgctta
     8461 tcgccctgaa tttcaataat tccatcttta actgctccgc cgcagccgca ttttttcttc
     8521 agttcagctg ccagtttcgt cagttcggca tcatcgagat cgacaccggt aatcaggcaa
     8581 acgcccttac ctttgcgtcc actggtctga cgctgaatac gcaccacacc gtcgccttta
     8641 gggcgtacag gggccgcttt tggctcatca atacgtccgg tttccgttga gtagaccaga
     8701 cgactgttgg aatcactcat catgcactcc gctgtaaaga ggcgttgatc gctttcagcg
     8761 tctgcgctgg atctaccgat tgcgttaccg ggcgaccaat caccatataa tcaacaccag
     8821 ccgacaacgc ctgttctggc gtcataatgc ggcgctggtc accagcatca ctcccctgcg
     8881 gacgaatgcc cggcgtaacc agtttgaact cctgaccgaa tacctgttta aagcgcacag
     8941 cttcctgagc agaacacacc acaccatcaa ggccacattt ttgcgtcagt gccgccagac
     9001 gttctgcata atctgcaggt gacagtgtca cgccaagatc ggccaggtcg ctggcttcca
     9061 tgctggtcaa cactgtcaca gcaatcaaaa gcggtgcatc tttgccaaac ggaaccagta
     9121 cctcacgcgc tgcggccatc atacgcgccc caccggaggc atgaacattc accatccaca
     9181 cgcctaagtc agccgcagca gcgacagcgt gtgctgcagt gttgggaata tcgtggaatt
     9241 tcaggtcaag aaagatatca aaaccacgct gttgaagttc gctcacaaac tgtggcccaa
     9301 acaatgtaaa catctctttg ccgaccttca gacgacaatc gcgtgggtcg atcttgtcga
     9361 caaaggccat cgcggcatcg cgattatgat aatcaagggc aacaactaca ggagaattcg
     9421 taacagcgcg ggaagaagat gaagcagtta acgtcatgac cagaccttct tgatgatggg
     9481 caccggaagt ggcgcgaaac aggtaaacgg cgagcattct acccgtggaa ataaaaaatt
     9541 aacagttgcg atttctcctc tggcaggctt ttccgcgccg tcggtgccgg agcacaatag
     9601 aacatcaata attagtatgt tgtaactaaa gtcggatttt tttaaaatta caggccatca
     9661 agaccgcgaa ttggtttaat ggttgaccag gcccgacaag acggacaatg ccagtagagg
     9721 gtgtatgcgg taaaaccaca tttctggcag cgataacggg gcttactacg taccttctcg
     9781 ccaaccatgt cacgcagcac catcagactc tctttggcac gcccttcttc tgcttcattt
     9841 aagtggtaat ccattaactt atggaacaca cgcatggtcg gatgacgctg aagctggcgt
     9901 gtaatataga cctgtgcggc ctcactaccg tcgcgcgctt cgatgatatc cgcaagcatc
     9961 aattcagcat cggcaccggt gttctcttcc accgcgcgct gcaggaattc tgcccattcg
    10021 gcagttttac ccaactgctg atagcatgtt tgcagcattt ccagcgtttc gctgaccagt
    10081 tctctgtcct gggatatgac acgttgcaaa ctttcgacgg ctttggcgta ttctcctttc
    10141 gccataaaca cgcgtcccat catgatggat acgcgggcgc tgtttttatc tgccgccgca
    10201 ccttttttta gcaaagtcat ggcacgatcg agatcgtcgc tggccatatg ctgcagagct
    10261 aactcacagt agaaatgggc aatttcgacg cgctgtttat ctttacccag cttcaccagg
    10321 cgttcggcaa catcaattgc tttctgccac tcgctggtag cctggtaaat ttgtagcagt
    10381 tgttgcagcg cgccaatgcg gaagtcagtt tcatctgtca gctgattgaa catgtcttcc
    10441 gcgcggtcgt ataacccggc agccatgtaa tcacgcccca attgttgaat cgccaaaagc
    10501 cgctgttcat aggtcagcga ggcgctttcc attagggtct gatggatgcg aatagcgcga
    10561 tcaacttcgc cacgcgaacg gaacaggttt ccgagcgtaa ggtgggcttc aacggtacct
    10621 gtatcctctt taagcatatc gagaaacagg tctaccgctt tatcctgttg attactaagc
    10681 aggaagttaa cccccgctac gtaatcacgc gacaagcggt tggcttcatc ttgcttgttt
    10741 tgttgcgcac ttctgcggcc catataccag ccataggcag cggctacagg caaaagcaga
    10801 aacaacaact ccagcataga aagttattcc ttcgacgcag acgagtgcgg cgctacagcc
    10861 acgtcagtcg cgggtgaaag ctggttttcc agtcgcttta ttttacgttc agcgcgcgcc
    10921 agggaaacac gaactcgcag ccagaacagg ccacaaatca accaaccgat agcaaacccc
    10981 gcagcaaaca ataccgccag caatgtggaa atacggtact ccccttgcgc taacagataa
    11041 ttaaacgtca cctgttgatc gttctgcgca cccaacgtga ccgaaatcac gaagatcgct
    11101 aacaccagta agaaaatgag taaatatttc acattacttc ccgttatgtg gttagagcga
    11161 ataaattatg gtcaacttac cgcaatagcc ctttaaagta ccattttcgc gccgtctgcg
    11221 aaatggcatc cacgcgcatt gttgatgatt taagggctaa attcggaaaa tcaaccagcg
    11281 ttaactttct tgttctcgtt gtgcaatttc gcgattttct tccgcaggtg gtgttaatgg
    11341 cccacaaatt cgttgcgcaa gccacgttgc caccgccacc agcgcccacg aaatcaacgt
    11401 agctacaacc agatcgcgtg gccaatgcat cccgagcagc aggcggcttc ccatgactcc
    11461 cgttgcccag accaacaaga tagcaatggt taacgttcgc cgacgcggcc acagcaaacc
    11521 aacggccagc agtgcccaac tggcagcaaa catcgtgtga ccggaaggaa aggcaaaccc
    11581 cgtctctttc tgccagtgtg aacgcaaata ttgtgggata tttttctctt cagccaactg
    11641 ttctttcacc agatttccgc gttctgctcg ctttaaagtg tagaactcat caaccggaat
    11701 atgatgtgtt ttttccagcc acataacaaa aggtcgtggt tcctggactt tgtctttgat
    11761 ccaggattta acgccttgtc ccataaggat tgccgccgcc agaatggcaa ataacataat
    11821 ggcagcctta atgcgaaaac gcagacacca gagaaaccaa ccgaataaaa tcaaatgtgt
    11881 aatgacgccc cagggctggg tgacagtttc agtaacccaa aaagccgctt ttagtaacca
    11941 actttgttct ccaggttgcc aacgccagcc agaaatccat acggctactg gcatgacaag
    12001 caatagtgca gctcccactg cggtacgtct ggcaatcgaa cgcatggcct ctcctttttg
    12061 ataagtccca caatcataac tgaaaatgcc agttccagga aaaattgaca gatttgtgcc
    12121 attccgtgaa cgatcgacgc gccgtgatta ggtgaacccc ttctcgttat ggcaaaataa
    12181 gccaatacag aaccagcatt atctggagaa tttcatgcag cttaaacgtg tggcagaagc
    12241 caaactgcca accccatggg gcgatttcct gatggtggga tttgaagaac tggcaaccgg
    12301 acacgatcat gtcgcgctag tctatggcga tatttccggg cataccccgg tacttgcgcg
    12361 cgtccattcc gaatgtctga ccggtgacgc cctgttcagc ttgcgctgcg attgtggctt
    12421 ccagctcgaa gcggcattga cgcaaattgc cgaggaaggc cgtggtattt tgctgtatca
    12481 ccgtcaggaa ggtcgtaaca ttggtctgct gaataaaatc cgcgcttacg cactgcagga
    12541 tcaaggttac gatactgtag aggctaacca ccagttaggc ttcgccgctg atgagcgcga
    12601 cttcactctt tgcgctgata tgttcaaact ccttggcgtc aatgaagtcc gcttgttaac
    12661 caataacccg aaaaaagtcg aaattctgac cgaagcaggg attaatatta ttgaacgcgt
    12721 accgttgatt gtaggtcgta accccaataa cgaacattat ctcgatacca aagccgagaa
    12781 aatgggtcat ttgctgaaca aataaccctc ttgcattgtg taattaattt gcttgccgga
    12841 agcaaaataa ccggcaagca aatactcgtt acttcaacat attacgaatg acataatgca
    12901 aaatgccgtc gttctggtag taggtcaact ccgtcgcggt gtcgatacga caacggcagg
    12961 gtacgacttc ctggctacca tccgcgcgcg taagcgtcac cggaaccgtc gcgccgggtt
    13021 gtagattttg cagatcacca atatcaatct tctcttcccc ggtaagctct aacgttttac
    13081 gcgtcacacc ttgtggaaat tccagcggca ggatgcccat gccaattaaa ttcgaacggt
    13141 gaattcgttc aaacgattcg gcaatcacaa cacgaatacc aagcagacgc ggaccttttg
    13201 ccgcccagtc acggctggag cctgatccat actctttccc ggcaatcacc gccagcggcg
    13261 tttgctcctg cttatagcgc attgcggcat cataaataga gacgacatcg ctgtcaggta
    13321 aatggcgcgt catcccccct tcaacgccag gcaccatttc attacggatg cgaatattgg
    13381 cgaaggtgcc gcgcatcatc acttcatggt taccacgccg cgaaccatag gagttaaagt
    13441 ctttccgctc aacaccccga ccttgtagat atcgacccgc cgggctgtcg ggcttaatac
    13501 tgcccgccgg agagatatgg tcagtggtga ctgaatcccc cagcattgcg aggatccgcg
    13561 caccgtgaat atcttccaca ggtgctggtg ttgcctgcat ttcatcaaag aaaggcgata
    13621 agcgaatata ggttgaatcc tcctgccaac cgtaggtatc ggatcgtgtg acgttaattt
    13681 ccttccactc ggctgtgcct tcaaaaactt ctgcgtactc tttgcggaac atttctgtgg
    13741 agacttggtc taccgcacgg gcaatttctt gtgccgatgg ccagatatct ttcagatata
    13801 ccgactcgcc tttgcgatca tggccgatag gctcagaagc caggttgata ttcatatttc
    13861 ccgccagcgc ataggcaacc accagcggcg gcgaggccag ccagttggtt ttaaccagcg
    13921 gatgaatacg gccttcaaag ttacggttgc cggacagcac tgcaccgacg gttaaatcgc
    13981 cttttttgat tgccgtttcg ataggatcgg gcaacggtcc agagttacca atacaggtgg
    14041 tacaaccgta tcccacaagg ttaaatccca gttcatcgag atacggtgtc agtttcgctt
    14101 ttgccagata atcagaaacg actttcgaac ccggtgccag cgacgctttg acccacggtt
    14161 gccgcttgag gcccagagtt acggcttttt tcgccagcaa gcctgcggcc atcagcacac
    14221 ttgggttaga ggtgttggtg cacgacgtta tcgcagcaat gaccacagcg ccatcaggta
    14281 actgatactg atgtccgttc ataacataat cgaccggctg gcgatcttta tgcgtggcat
    14341 tcacttccag ttcgctactg gcagcaaatg cttttggtac atcgggcagt gcaacgcgat
    14401 cctgcgggcg ttttggccct gcaaggctcg cttcaacgtc attcatatcc agttctaaca
    14461 cactggtaaa aattggttca tcgcccggat tacgccacat gccctgcgct ttggcatatt
    14521 tttcgaccaa ctcgacctga tcttcactgc gcccgcttaa acgcatgtaa tcgagggtga
    14581 cagcatcgat tgggaagaag ccacaggtgg caccatattc tggcgacata ttggcaatgg
    14641 tggcgcgatc cgccagcggt agtgaatcca gcccatcacc ataaaattcg acgaatttcc
    14701 ccaccacgcc atgtttgcgc aacatttggg taacagtgag aaccaggtct gtggcggtaa
    14761 taccttcacg taattttccg gtaagtttaa agcccactac gtccgggata agcatggaaa
    14821 ccggctggcc taacattgcg gcttctgctt cgatgccacc aacgccccac cccagcacgc
    14881 caaggccgtt gatcatggtg gtgtgcgagt cagtaccaac gagtgtatcc ggataagcaa
    14941 tccattcacc gtcctgcaat tcgctccaca ctgctttgcc gagatattcg aggttaacct
    15001 gatggcaaat gcctgtgcct ggcggcacga cgctaaaccg actgaacgcc tgctttcccc
    15061 atttcaggaa cacataacgt tcgtggttgc gctccatttc caggcgtacg ttttcttcaa
    15121 atgcctcatc atcaccaaaa cgatcgacgg tcaccgagtg gtcaatgacc aagtcgaccg
    15181 gtgagagtgg gttaaccttt gcagtatcgc cgccgaggcg tttaaccgct tcgcgcattg
    15241 ccgccagatc aacaacggca ggtacgccgg taaagtcctg catcagcact cttgccgggc
    15301 ggtaggcaat ttcacggtca gcatgggcat ttttcagcca tcccgccagc gcgtggatat
    15361 cctcttcggt aaccgattta ccatcctgcc aacgcagcag gttttcgagc aaaactttga
    15421 gtgacttggg tagacgggtg atatcgccca gtgatttggc agcaagcggc aagctgtagt
    15481 agtggtaagt tttatctttg gcctgcaacg tgtccttact ggcttctcgt agggttgacg
    15541 acatagctcc tccttaatga cagggttgcg aagccctgat tctcttcagg gtgaatatta
    15601 aagataacac aaacatcgcg taacgatttg ataacaaccc aaattgataa aagaggagaa
    15661 accttgaggc aaagcaaaca gcggggtcag ggatcagtga gtaatgatcc agaggagttc
    15721 ggcccagaag agcatcgaca gagaaaatgc gcccatccag gaccaatatt tcatatttgt
    15781 gttgatcata ggctacaccc ttaacggtta ttactttgtg ttgagataac tcaacctgtt
    15841 attgctgcgg tgtattaaaa gccctgtaat tcattaaaaa tgggtaataa gaatgatgat
    15901 aattattgtt tcggctgatc atcttcagca aacagttcta taaaggcttg ttgctgcgat
    15961 gttagttgcc agtcgtctgg aataacgacg gaacattccc gttttgcttc tggttcattg
    16021 tcactatgct gacataatgg tgacgactcc agttgctcct gaccgaccat agttatcccc
    16081 acaatttcca aataaacagt gcgacaacca cccagaaaag tgcagaacca agaaaaaccg
    16141 ccagccaggc tttacgtttt aattcaggat tgcggcgggg ttcctgattt ccagaaggca
    16201 ttgctaacct catataatcg ccatcactta tcagcaagac gttgaacgat tggtgtaatt
    16261 aatcatacga tgagacaaat cctaaagaaa ctttagtcaa aaatccagcg aatttacgaa
    16321 tcgtctccag tgaataattc aatcttttga cctgaaatag atatttgaaa actggagttt
    16381 gcgaggtgtg taattagaca ctactttaca ggtaatttac cctataaata tgagggataa
    16441 aaatattgtg caccggatac aaataagtaa caaaggagaa tatattccgg caccttcgct
    16501 acataaaagg tgccgaaaat aacgcaagaa attatttttc cggcagtttt atatctttaa
    16561 acatgacctc aatttcttca ttagagcgca atgcgacagc cgcatcaacg acatcacgcg
    16621 ttaaatgcgg tgcaaaacgc tgaatgaaat catacatata actacgcaaa aaagtactac
    16681 ggcgaaaacc aattttggtt gtactgtggc tgaagatgtc gtgagcatca acacgcacaa
    16741 ggtcgggatc ggcgaccgga tccaccgcca tgctggcaat gacccctacc cccagcccta
    16801 accggacgta agttttaatg acgtcagcat ccgttgccgt gaagacgata cgcggcgtta
    16861 accctgcgcg gttaaaggca gtatccagtt ctgaacgtcc ggtaaagccg aaggtatatg
    16921 tcaccaacgg atattgcgcc agttcttcaa tggtaatggc ttttttacct gccagcgggt
    16981 gatccggagt gactactata gcccgattcc agtggtagca cggtagcatc actaaatctt
    17041 catacagatg cagcgcttcc gtggcgatag cgaaatcggc attgccttta gagacggcat
    17101 cagcaatttg tgtcggcgag ccctggtgca tatgcaaaga aacgcgagga taacgctcaa
    17161 taaagccttt gataacgttt ggtaacgcat agcgtgcctg agtatgcgtg gtggcgatat
    17221 acagcgagcc tttatccggc caggtgtgct ctccggcgac cgattttatg gcatcgactt
    17281 tcgacagaac ttcacgagcg atacgaatta tttcttgtcc tgctggcgtt acctgcgtca
    17341 ggtgcttgcc gcttcgggaa aaaatttgaa tgcctagctc gtcttccagc attctgactt
    17401 gtttactgat cccgggttgt gatgtgtaaa gtccttccgc cgttgatgag acattcaggt
    17461 tatgattgac cacctcaaca atatagcgaa gttgttgtaa tttcatgtta aaccatccac
    17521 ttagacgcca tcagaacatc gttttttcgt cacaccaggc gggtgactaa tacgatttaa
    17581 taataa
//
LOCUS       DADTFG010000102        17510 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.102, whole genome shotgun sequence.
ACCESSION   DADTFG010000102 DADTFG010000000
VERSION     DADTFG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.102"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(104..2551)
                     /gene="glgP"
                     /locus_tag="J1138_003180"
     CDS             complement(104..2551)
                     /gene="glgP"
                     /locus_tag="J1138_003180"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBA8454943.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2570..4003)
                     /gene="glgA"
                     /locus_tag="J1138_003181"
     CDS             complement(2570..4003)
                     /gene="glgA"
                     /locus_tag="J1138_003181"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBA8454944.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGLASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(4003..5298)
                     /gene="glgC"
                     /locus_tag="J1138_003182"
     CDS             complement(4003..5298)
                     /gene="glgC"
                     /locus_tag="J1138_003182"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBA8454945.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5316..7289)
                     /gene="glgX"
                     /locus_tag="J1138_003183"
     CDS             complement(5316..7289)
                     /gene="glgX"
                     /locus_tag="J1138_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBA8454946.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGVFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7286..9472)
                     /gene="glgB"
                     /locus_tag="J1138_003184"
     CDS             complement(7286..9472)
                     /gene="glgB"
                     /locus_tag="J1138_003184"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBA8454947.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYCHHKAMHELDFDSY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9745..10848)
                     /gene="asd"
                     /locus_tag="J1138_003185"
     CDS             complement(9745..10848)
                     /gene="asd"
                     /locus_tag="J1138_003185"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBA8454948.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     ACTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11040..11633
                     /gene="yhgN"
                     /locus_tag="J1138_003186"
     CDS             11040..11633
                     /gene="yhgN"
                     /locus_tag="J1138_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBA8454949.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11690..13030)
                     /gene="gntU"
                     /locus_tag="J1138_003187"
     CDS             complement(11690..13030)
                     /gene="gntU"
                     /locus_tag="J1138_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBA8454950.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13034..13561)
                     /gene="gntK"
                     /locus_tag="J1138_003188"
     CDS             complement(13034..13561)
                     /gene="gntK"
                     /locus_tag="J1138_003188"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBA8454951.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13700..14695)
                     /locus_tag="J1138_003189"
     CDS             complement(13700..14695)
                     /locus_tag="J1138_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GntR"
                     /protein_id="HBA8454952.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14789..15484)
                     /locus_tag="J1138_003190"
     CDS             complement(14789..15484)
                     /locus_tag="J1138_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBA8454953.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(15607..16644)
                     /locus_tag="J1138_003191"
     CDS             complement(15607..16644)
                     /locus_tag="J1138_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA8454954.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            16977..17465
                     /gene="yhhY"
                     /locus_tag="J1138_003192"
     CDS             16977..17465
                     /gene="yhhY"
                     /locus_tag="J1138_003192"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA8454955.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALLNGEYVDAYYMAR
                     VK"
BASE COUNT         4296 a         4764 c         4372 g         4078 t
ORIGIN      
        1 agaataacat acatcaaagt tattgtttat tgtgtataga aataccgtga gttccactct
       61 gtattacttc agcggaacac acggtattta gccgtatttg aacttacaat ctcaccggat
      121 caatatgcca gatatgatcg gcgtactctt tgatcgtacg gtcagaagag aagtagccca
      181 tattggcaat gttcagcatc gcttttgcgg tccactcttc ctgacgctcg tagagttcgt
      241 cgactttatc ctgacaatcg acatagctgc gataatccgc cagtacctgg tagtgatcgc
      301 cgaagttgat cagcgaatcg accagatcgc gatagcgacc cggatcttcc ggactgaata
      361 caccgctgcc gatttgcgtc agcacctgat gcagctcctc atctttctcg tagtattcac
      421 gcggtttgta gccctgacga cgcagttctt ccacttcttc cgctgtgtta ccaaagataa
      481 agatattgtc agcaccgaca tgatccagca tctcgacatt cgcaccgtcc agcgtaccga
      541 tagtcagcgc gccgttaagc gcaaacttca tgttactggt gccggaagct tccgtccccg
      601 ccagcgaaat ctgttcagat agatcggccg ccggaatgat caactgcgcc aggctaacac
      661 tgtagttcgg gatgaacacg actttcagct tgtcgccaat ctgcggatcg ttgttgatca
      721 ctttcgctac gtcattgatc aaatgaataa tgtgcttcgc catgtaatag gccgaagccg
      781 ccttaccgcc aaaaatattc acgcgcggta cccacttcgc atccgggtcg gccttgatgc
      841 ggttatagcg ggtgatcacg tgcaacacat tcatcaattg acgtttgtat tcgtgaatac
      901 gtttgatttg cacatcgaac agcgctttcg gatttaccac cacattcagc tgctgggcga
      961 tatactccgc cagacgcttt ttgttctcca gcttcgcctg atgcaccgcg tgattaacca
     1021 ttgggaaatc acagtgttgt tgcagatcat taagcaggct aaggtcggtg cgccagttgc
     1081 ggcccaggtg ttcgtccaac acggctgaaa gcgatgggtt cgctaccgcc agccagcgac
     1141 gcggcgtcac accgttggtg acgttggtga aacgacccgg gaagattttc gcaaagtcgg
     1201 caaatagcga ttgcaccatc aggttagagt gcagctccga tacaccgtta actttgtggc
     1261 tcaccacaac cgccagccag gccatacgca cacgacgacc gttggattca tcgatgatcg
     1321 acgcccgtcc cagcagatcg gtatcgttcg gatactgttc ctgcaaagtt ttcaggaaat
     1381 agtcgttgat ttcaaagatg atctgcaggt gacgcggcag aattttaccc agcatatcga
     1441 ctggccaggt ttccagcgcc tcgctcatta gcgtgtggtt ggtgtaggag aagacctgac
     1501 aacacacttc aaacgcgtcg tcccagctaa attggtgctc atcgatcagc agacgcatca
     1561 tctcaggaat cgacagcacc ggatgggtat cgttgaggtg aatcgcaatt ttatccgcca
     1621 ggttatcgta ggttttatgc aactgataat ggcggcttaa aatgtcctga atggtcgagg
     1681 aaaccaggaa gtattcctga cgcagtcgca gctcacgccc ggagtaggtg gagtcgtccg
     1741 gatacagtac gcgagatacg ttctcggagt ggtttttatc ttccactgcc gcgaagtagt
     1801 cgccctggtt gaatttacca aggttaattt cgctactggc ttgggcactc cacaaacgca
     1861 acgtgttggt cgcgtcggtg tcgtaacctg ggattatctg atcgtaagcg actcccagaa
     1921 tctcttcggt ttcaatccag cgcgtttttt taccttcctg ctgaatgcga ccgccaaaac
     1981 ggactttata gcgcgtgttg tggcgtttga attcccacgg gttaccgtat tccagccagt
     2041 agtcaggcga ctctttctgg ctaccgttaa cgatgttctg cttgaacata ccgtagtcat
     2101 agcggatgcc gtaaccgcgc cccggcaacc ctaacgtcgc cagagaatcg aggaagcaag
     2161 ccgccagacg gcccaggcca ccgttaccga ggcccgggtc attttcttca tcaatcagct
     2221 cttcgagatt taatcccatc gcttccagtg cgccctgtac atcttcgtaa attcctagcg
     2281 acaacatggc gttggagagc gtacggccaa tcaaaaactc catcgacagg tagtaaacct
     2341 gacgagtttc ttgcgacaac tgggcacggt ttgaacgtaa ccagcgctcc acgagacgat
     2401 cgcgcacagc aaataacgtt gcgttcagcc attcatgttt attggcgacg accggatcct
     2461 ttccaatcgt aaacatcagc ttgtaagcga tagagtgctt aagagcttct acgctaagcg
     2521 tgggcgatga atatgtaaac ggagcattca tataggcgtt tcctgaaaac tatttcaagc
     2581 gatagtaaag ctcacggtac gacttcgccg cgacctgcca gctaaaatcc attgccatag
     2641 cctgacgttg cacaaaccgc cacagtgaag gacgggacca cagtacaaaa gcacgtcgaa
     2701 tagcccgtaa cagcgaccag gcattactat cttcaaagac aaacccactg gcgaggccat
     2761 ctgcaaggtt ctcaagagaa cagtcagaaa ccgtatcagc aagcccaccg gtgcgccgca
     2821 ctaacggcag cgtaccgtac ttcaatccat aaagttgcgt taagccgcac ggttcaaaac
     2881 ggctgggcac cagaatgacg tccgcgccgc ccataatgcg atgcgaaaat gcttcgtgat
     2941 agccaatctg aacgcccacc tggccggggt attccgctgc cgccgcaagg aaaccttcct
     3001 gcagcaccgg atcgcccgcg ccgagtagcg ccagctgccc gccctgctcc agaagaccag
     3061 gtaaggcttc cagcaccagg tcgagacctt tctggctggt aagacggctc accactgcaa
     3121 aaagcggcac tttatcgtca accttaagcc ccattgcgat ttgtaactgg cgcttatttt
     3181 ccgctttatc ttccaacgta tcgcgggtgt aacgcgaggc caacagtaag tccgtctctg
     3241 gactccagat tttctcgtcc acgccgttca gtacgccgga aagacgccct tcacggtgac
     3301 gctgttgcaa cagaccttcc ataccgtagg caaactgcgg ttcggtgatc tcgcgagcgt
     3361 aggttgggct gactgccgta atgtgatcgg catagtacag accggccttc aggaaagaga
     3421 tttgtccgtt gaattccagc ccatgaatat taaagaatga ccatggcaat tggatgtcat
     3481 tcatgtgatg tgcataaaac atgccttgat aggccaggtt gtgcacagta aacaccgact
     3541 tcgccggacg cccgcgcgcc gccagatacg caggcgcaag gcctgcatgc cagtcgtgcg
     3601 catgcaccac atcaggacgc cagaatgggt caagcccgct ggccatttct gccccaaccc
     3661 accccagcag cgcaaaacgc aatacgttgt cggtataggc aaataagttg gtatcgtgat
     3721 acgggcttcc cggacgatca tagagatgcg gcgcgtcaat caggtatatg ccaaccccgt
     3781 tgtaatgacc gaacaacagc gtgatatgtc cggcgaaggt atcacgacgg gatactacct
     3841 gcgcatcggt cacgccacgg cgaatatcgg gaaatgcagg caacagtacg cgagcgtcaa
     3901 cgccgtctgc gatttgtgct gcgggtaatg ccccaataac atcagccaga ccgccggttt
     3961 taagcagcgg gaacatctct gaacatacat gtaaaacctg cattatcgct cctgtttatg
     4021 ccctaacttc cgtagcattt cgcgcgttac cagcacgatg ccttcttctg aacgatagaa
     4081 acgacgtgca tcttcctctg cgttttcacc aatcaccatg ccttccggaa taacacaagc
     4141 acgatcgatg acgcagcggc gcagacggca cgagcgacct acccatactt ccggtaacaa
     4201 tacggcggaa tcaatgttgc agaatgaatt cacgcgaacg cgcgagaaca gaacggactg
     4261 caccaccacc gaaccggaga tcacacaacc gccggatacc agtgagttaa gggtcatccc
     4321 gtggctaccg gagcgatcct gcacgaattt cgctggcggt aatgattcat tgtaggtgcg
     4381 aattggccaa ttgcgatcgt acatatccag ttccggcacc acagaggcca gatcgaggtt
     4441 cgctttccag taagcttcca gcgtacccac atcgcgccag tacggctcgg catccgggtc
     4501 ggattgtacg caagagagcg ggaacgggtg cgcataggcc agaccggctt cggtgatctt
     4561 gggaatcaaa tctttgccaa agtcgtggct ggagttctca tcgcgatcgt cttcttccag
     4621 cagttcatac agatagtcgg cgtcaaagac gtaaataccc atactcgcca gagatttgct
     4681 cggatcgttc ggcattgacg gcgggttagc tggtttttca acgaattcga taattttatc
     4741 gttctcatca accgccataa cgccaaacgc ggaggcttct tcaatcggta ctggcataca
     4801 agcaacggtg caacgtgcgc ctttttcgac gtggtcgata agcatacgcg agtagtcttg
     4861 cttgtagata tggtcgcccg ccaggatcac cacgtattcc gctttataac ggcggataat
     4921 gtcgaggttt tgggtgaccg catctgcggt gccgcgatac cagttttccc ctttcattct
     4981 ctgctgtgct ggcagcagat cgacaaactc gttcatttct tcattgaaga atgaccagcc
     5041 gcgctggatg tgctgcacca gagtgtggga ctggtactgg gtgatcacgc ccatacgacg
     5101 gatcccggag ttgatgcagt tagacagcgc aaagtcgata atgcggaact taccgccgaa
     5161 gtgtacggcc ggttttgctc gcttattggt taaatccttc aggcgggtac cacgtcctcc
     5221 cgccagtatc agggcaacag atttcaatgg cagctggcgc gccaacatta agtgatcgtt
     5281 cttctctaaa ctaaccatga ctaactcctt ttttatcatc tctggaacac acacaatccg
     5341 tgtgcaggtc cctgccagac agccgtaatc actgggttat cctctccagc gaatggggga
     5401 atggcgtgcc actccccagc aggtaaaaca atctctgtta cctcaagcgt ggcgttaatt
     5461 gcgatcaaaa agcgatccga gagcagaatt tgcagctgtt tcggcccgtt ttgccactca
     5521 tccgtgctta aaggttgagc atatcgattt agccaacgga cattgccgtc gccttcttcc
     5581 caccagcgat tctccatcaa agcgggaatg cgcttgcgca gatggattaa cgcggcggta
     5641 aatgcggtta aaccactgct tgcctgcgac cagtccaacc aggttaattg gttatcctga
     5701 cagtaggcat tgttattgcc atgctggctg tgaccatgtt cgtcaccggc cagtaacatc
     5761 ggcgtaccct gggagagcaa caacgttgtt aacagggcgt gaatgctgtc gcgccgccgt
     5821 tcaaccagat caagagtacc gcctaaccct tctttaccat gattgttact gtaattgttg
     5881 ttggtcccgt cgcgattttc ttctccgttt gcttcattgt gtttatggtt gaagcaaacg
     5941 cagtcgcgaa gcgtaaaacc gtcatgcgcg gtgacgagat taatcgcggc actcggcaga
     6001 cgaccattac gtttaaaaac atcgctggag gcagcaaaac gcccagcaaa cactcccaga
     6061 ggtaaatcat aatgcagcca gaaacgacgg gctgcatcgc ggaaatgatc gttccactcg
     6121 gcaaacagcg gcgggaaatt tcccacctga taaccaccag gagcgatatc ccacggttca
     6181 gcaattaact tcacctgcga gagcaccggg cagttctgga tagcggtaaa caacggcgca
     6241 tcctgacgaa actctggcgt acggcccatg actgccgcca gatcaaagcg gaaaccatcg
     6301 acgtggcagg tttctaccca ataacgcagg caggcgctgg cataatctac caccgccgga
     6361 tgactcaaat tgagcgtgtt accacaaccg gtccagttgt gataatcgcc gtcttctctt
     6421 atccaataat agctacggtt gtcgatccca cgcagcgaga ataacgggcc gtcgaggtcc
     6481 agttccgcac tatggttgag cacgatatca agaatgactt cgatacccgc tttatgcagt
     6541 gctttgattg catcgcgaaa ctcatccagc gccgtttctg gcgagcaggc atacgccgga
     6601 tgcagcgcaa acatcgccac cgggttgtaa ccccagtagt tactaagccc catacgttgc
     6661 agacgtggtt cgctggcaaa ttgtgccacc ggcagcagtt ccagcgcggt aatgcccaac
     6721 tgtttcaaat agttgatcat caccggatgc cccagggctt tataagtgcc acggatctcg
     6781 accgggatct ccgggtgcag atacgttaat cctttgacat gggcttcata aatgatggtg
     6841 ctgccccacg gcgtgcgcgg cggggcatca tcctcccagt catagtgatc aaccaccact
     6901 acgcatttcg gcgcaatggc ggcgttgtcg cgatagtcag gttcattatg accggcgtgc
     6961 agcagcgggt tatctttaaa ctccccgtca atttgccgcg cgcaaggatc aatcaacaac
     7021 ttcgccgggt taaagcgatg cccctcggcg ggttgccaag ggccatgaac gcgataacca
     7081 taacgcaaac ccgggcgcgc atccggcaga taaccgtgcc aaatgtcgcc actgtgccct
     7141 ggcaagtcat agcgatgttc ctggccattg gcatcaaaga cacacagctc tacccgctcg
     7201 gcatgagcgg agaaaagtgt gaagttgacg ccctgaccgt cgtaatgcgc gccgagggga
     7261 gtgggtttgc caatggcgag ttgtgtcatt ctgcctcccg aaccagccag atagtggcca
     7321 gaggtggtag cgtcaggctc agtgaatgct gacgaccgtg gctggcaatc tcatcactgt
     7381 gtactgcgcc gccattgcct gcattactgc cgtgatagtg catggaatcg gtattgagga
     7441 tttcacgcca tttacccggc tggttaatac cgaagcgata atcatgacgc ggcaccggtg
     7501 taaagttact ggcaacgatg atttcgttac cctctttatc gcgacgcaca aagatcagca
     7561 ccgagcgttc tttgtcatcc accaccagcc attcaaagcc gtacgagtca aaatccagtt
     7621 catgcattgc tttatggtgg cagtaggtgt ggttcagatc gcgcaccaga cgctggacac
     7681 cgtggtgcca gttatcgccg ccttccaaca gatgccagtc gaggctggcg tcatggttcc
     7741 actcgcggcc ctgggcaaat tcgttcccca tgaacagtag tttcttgccc gggaatgccc
     7801 acatccagcc gtagtaggcg cgcaggttag cgaatttctg ccatgcgtcg cccggcatcc
     7861 ggtcgagaat cgatttttta ccgtggacca cttcatcatg cgacaacggc aggacgaagt
     7921 tttcagtgta gttgtagagc atcccgaagg tcagtttatc gtgatgatac tgacgataaa
     7981 tcgggtcgag cttcatgtag tccagggtgt catgcatcca gccgaggttc cacttgtacc
     8041 agaagcccag accgcccata tcctgcggac gagaaacgcc agggaaatcg gtagactcct
     8101 ccgccattgt cactgcaccg gaaacctgct caccaagaat acggttggta ttacgcaaga
     8161 attcaatcgc ttcaagattc tcgcgaccgc caaattcgtt cgggatccac tccccctctt
     8221 tacggctgta gtcgcgataa atcattgacg ccaccgcatc gacgcgcagc gcatcaatac
     8281 caaaacgttc aatccagtaa agtgcgttac cgacaaggaa gttactgact tcacggcgac
     8341 catagttgta gatcagcgtg ttccagtcct gatgatagcc ttcgcgcgga tcgctgtgtt
     8401 catacaagtt cgtgccatca aattcggcaa gcgcaaagtc atcggtcggg aagtggcctg
     8461 gcacccagtc gagaatcacg ttcagaccag ctgcgtgtgc ggcatcaatg aaataacgga
     8521 agtcgtcgcg agtaccaaaa cggcgggtcg gtgcatacag gccggttggc tgataacccc
     8581 aactgccatc gaagggatgc tcattaatgg gcagtagctc gagatgggta aagcccatcc
     8641 atttagcata aggcaccagt tgatcggcca gctcgcggta gctcaaccag aaattgttgt
     8701 cggtgtggcg acgccaggag ccaaggtgaa cttcataaat agagattggc gcatcaaact
     8761 gattcgcttt tttgcgctct tcagtctgca caaccttttc cggcagcccg caaataagag
     8821 acgcggtttc cgggcgcatt tgcgcttcga aggcataagg gtcggacttc agacgcaagt
     8881 tgccattggc atcaatcatc tcgtatttat agagctgacc gttatgcgcc ccagggataa
     8941 acagttccca gattccgctc tctttacgca gtcgcatcgg atgacggcga ccgtcccagt
     9001 agttgaattg cccaaccacc gagacccgac gggcgtttgg agcccagaca gaaaaacgcg
     9061 tacctgtgac gccatccata gtatctgcat gcgcgcctaa ggtttcatac ggacgtaggt
     9121 gagtaccttc agataatagc caggcatcca tttcctggat tagcgggcca aaacggtaag
     9181 gatcatcaat caggttttgc tgaccatgcc agacaacagc caactgatag cggaaaaaat
     9241 tcttacgtcg cggaatgaca ccgctaaaga atccccgtga gtcgagacac tccagttttg
     9301 cgagtttgcg cccggttttc ggttcaatca cccacacatc ggtagcgtcg ggtaaaaggg
     9361 cacggacttc cagtcccgcg gtggttttat gcatccccag tacggaaaaa ggatccgcaa
     9421 aatggcctgc aattagcgcg ttaatcacgt ctctatcgat acgatcggac atgcttgtct
     9481 tcctgtttta ttgtgtcacc ccatccaact gaatttttga cttctggttg tgacattttt
     9541 ttgacctgaa cggcgcagca ctctgtgcat cctctctgcg tcgtcctcac ttcaggtaag
     9601 gctgtgaata ctcgtgtatt ccgccaccct taaagaatag ccaatgctct atttaactcc
     9661 cggtaaatca tgaaacatct gcgcttactc ctgtattacg cactagcagg ggcggcatcg
     9721 cgccccagat ttaatgaata aagattacgc cagttgacga agcatccgac gcagcggctc
     9781 cgcggccccc cacagcagct ggtcgcccac ggtaaaggct gacaggaact ccggtcccat
     9841 atttagctta cgcaggcggc ctaccggcgt ggtcagcgtg ccggtaacgg cagctggggt
     9901 tagctcacgc atagtgattt cccgatcgtt cggaacaact ttcgcccacg gattgtgcgc
     9961 agccagcagt tcttccacgg tcggaataga cacatctttt ttcaatttaa tagtgcatgc
    10021 ctggctgtgg cagcgcaatg ccccgacacg cacacataaa ccatctaccg gaattacgga
    10081 agatgtgttg aggatcttgt tggtttccgc ctgccctttc cactcttcgc ggctctgacc
    10141 gttatcaagc tgtttgtcga tccacggaat caggctaccc gccagcggca cgccaaagtt
    10201 atctaccggc agctcaccgc tacgggttaa ggttgtgact ttgcgttcga tatcaagaat
    10261 agcagaggac gggttcgcga gttcatctgc cacatggcca tacagatggc ccatttgggt
    10321 taataactca cgcatatgtc gcgcaccacc gccggaagcg gcctggtagg ttgcaacgga
    10381 cacccaatca acaagatcat tggcgaataa accccccagc gacatcaaca tcaggcttac
    10441 ggtacagtta ccgccaacaa aagtcctgat gccattattt aatccgtcgg taatgacgtc
    10501 ctgattgacg gggtcaagaa tgatgatggc gtcatctttc atgcgcagag atgatgctgc
    10561 gtcaatccag taaccttgcc atccgctttc acgaagcttt ggatagattt cgttggtata
    10621 atcgccgccc tgacaggtca caatgatatc gagggccttt agcgcctcca gatcaaaggc
    10681 atcctgaagt gtgccagtgg ttccgccaaa agacggcgca gcctgaccaa gctgagaagt
    10741 agaaaagaag acagggcgaa tggcgtcgaa gtcgcgctct tcaaccatgc gttgcatgag
    10801 aacggagccg accataccgc gccagccgat aaaaccaaca tttttcataa gcgttttttc
    10861 ctgcaaagat gtgtgctgta taaatgtgcc ggtctcctcc tggcacatct ttcaccatac
    10921 aaaaagcagc caaagtcgca agtgaaatta atcaatgata gcgaagccat cagtaatgcg
    10981 acttatcctg ctttgttagc acgcagaaag tccgcggcaa ttatcaggga atttgagtta
    11041 tgaatgaaat catttctgca acagttttat tgatcctgat tatggatccg ctcggaaacc
    11101 tacctatttt catgtccgta ctgaaacata ctgaaccgaa aagacggcgg gcaatcatgg
    11161 tgcgagagtt gcttattgct ctccttgtga tgctggtgtt cctgtttgcg ggcgagaaaa
    11221 ttctggcatt tcttagcctg cgggcagaaa ccgtctccat ttctggcggc atcattctgt
    11281 ttctgatcgc cattaagatg attttcccca gcgcttcagg aaatagcagc gggcttccgg
    11341 caggtgaaga gccgtttatc gtgccgttgg caattccgtt agtcgccggg ccgactattc
    11401 tcgccacgct gatgttgttg tctcatcagt acccgaatca gatgggacat ctggtaattg
    11461 ctctgctgct ggcctggggc ggcacctttg tcatcctgct acagtcttcg ctatttttac
    11521 gtctgctggg cgagaaaggg gtgaacgcac ttgaacgcct gatgggattg attctggtga
    11581 tgatggcaac tcagatgttc ctcgacggca ttcgaatgtg gatgaagggg taacgaggag
    11641 agtttttcga aaagagcccg gtagcgtcag aactaccggg taaacaaact taactcaaca
    11701 gctggaacgc aatcatccca acgatggcac cgacagtgcc gaggatggtt tccatcatgg
    11761 tccaggtttt aagcgtttcg gcttcggtcg cgccggtaaa tttaccgaac aaccagaaac
    11821 ccgcgtcgtt aacgtggctg acaacaatcg aaccaccggc gatacaaatc gacagcgccg
    11881 ccatttgcgc accggagtag ttcagttgtt caatgaccgg catcaccaat cccaccgccg
    11941 ttaaacaggc aacggtggca gaaccctgaa tgatgcgcac tgcagctgcc agcacgaagc
    12001 aggtgatggc aatcggcagg cccatgccgg ttaacgcttc gcccagtgcc ggaccgacac
    12061 cagagtcaac cagcacctgt ttgaacacgc cgcccgcacc aatcaccagc agaatgatcc
    12121 ccgccggttg cagcgcgtga ccgcaaatct ccatcacttt gtcttttggc ataccctgac
    12181 gcattgccag accgtaaatc gccaccaggc aagcaaccag aatcgcggta aacggatgac
    12241 caataaactc gaaccattcg taagcagtag agccttctgg cacaaaacgc gcggcaatgg
    12301 ttttcagccc caccagcacc agtggcaaca ggatcagcga caggctgaat ccgaaggacg
    12361 gcattttgcc ttcgccgaga tgcggttcgc tgatgtcgtc aggaatatgc aactccacgt
    12421 agcggctgat gaaattaccc cacagcggcc cggcaataat cattcccgga attgccgcgc
    12481 acaggccaat caggatcatc cagccaaagt cggcgttcat ctgcgatgcc agcagcattg
    12541 gcgctggccc aggcaccagg aacgcagccg ctgccgccac gcctgcaaat aatgggatta
    12601 ccagcttcac caggttcgtg ccggtgtggc gcgccattga gaaagcaacg ctaatcagca
    12661 gaacaatcgc cacttcaaag aacagcggta gcgcacaaac cagccccgca aggccgatgg
    12721 cataatgcac gcggctgtga ccgaaggatt tgagcatttt gacagcaatc tgatcgaccg
    12781 cgccggtttc atgtaagatc ttaccaaaca tggctcccag ggcgacaacc accgccagga
    12841 agccgagggt gcctcccatc cctttttcca tcgtcgctgc gattttatcg agcggcatac
    12901 cggaaaaaag gccagccccc atggacacca ccattaaagc caggaaagcg tgcatacgcg
    12961 ccttcatgac taaaaacagc agcagtaaaa cagaccctac tgctgttaaa acaagcgtta
    13021 atgtagtcac tacttatttg ccttttttaa taacctcaat ggtgcttgcc acaacacctt
    13081 ccagcggttg atcaatatcc accaccagta catcggtttc gtccgcaccc ggctcctgca
    13141 gcgtttcaaa ctgcgtcacc agcatttggg ttttaaagaa atggcctttg cgcgctttca
    13201 ggcggctttc aatcacatca aaatcgcctt tcaggtagat gaaagagaga ttaggattac
    13261 cttcacgcag caagtcgcga tagtgttttt tcagtgcaga acagacgatc agcgatactt
    13321 tattggtgcg ctgcatagca aacgcggcgt cgttcagcgc ctgcaaccac ggtttgcgat
    13381 cgtcgtcatt cagcggttcg ccagacgcca ttttttcgat attgcagcgc ggatggagga
    13441 aatcgccatc aagaaacgcg gcatgaagtt gatgcgccac ttcactggcg accgcagatt
    13501 tgccgctgcc cgatacgccc atcaagacgt aaatgtggtg atcatggtta gtcgtgctca
    13561 aagtggtgcc cccacaatac aagaattaaa aatgttacgg gtaactgtta tcggtaacat
    13621 tgtccagccg gacaatatca gaagcaatat ccatccgtgc cttaagtgta taagtgtgag
    13681 ctacttcaaa tttgtaggct taaatagatc cgcccggtga caaggtgaaa cctaaatcta
    13741 acattttcgg tgtcacagat tcgccacgaa tacgcgccag caggcgctca gcgccaatgc
    13801 tgcccatccg ctcacgcggc gtcagcacac tcgctagccg tggctccatc acctgaccaa
    13861 tgtcatgacc gtggaaaccg gcaatcgcca tatcgtcagg aactttcaac cccagacgct
    13921 gacattcaaa cgccgcgccg accgccaggt catcattagt acagaacacg ccatccagct
    13981 gcggatattc ccgccgcgcc tggcgaatca gttcaatacc ggaagagtaa gaagaagatt
    14041 gctcaaccat cacgctatac ggcaccagac ctgcatccag cattgcctgt tcgtacccct
    14101 tctgtttgat gatagtacgt tcgtcgagac gtgcgccgag ataggcaatg tggcgatgcc
    14161 cgcgagcaat aatggcagtg gtcatctggc gtgctgcttc aaagttatca aaaccgacgg
    14221 caatatcgag gcatggcgac ttgctgtcca tcagttccac cacgggaatc cccgccactt
    14281 caatcatctt taaggtgcgc ggcgtgtggg tacgttcggt gaggatcagg ccgtcgatat
    14341 tccaggagag catggattcg aggcgttctt gctccatttc cggtttataa ccgtagtgcg
    14401 ccagcatggt ctgataaccg tgcgcgtcgg tgacgctttc gattccgcgt aatacttccg
    14461 cgaaaacctg gttggtgaga gaaggtaaca ggacgccaat cgcccggctg gtggcgttag
    14521 agaggatatc gggcgcacga ttgggaatat agcccagttc atcaagagcg gcggcaatct
    14581 tgccgcgtag agcgacggaa acctgctccg ggttgcgtaa aaaacggctg accgtcattt
    14641 tggtcacgcc tacacggtca gccacatcct gaagtacggg tcttttcttt ttcatcgtcc
    14701 tgaaaggtac aaaagggata gatttcttta gtttaacacg gacgcatcac aaccttcccg
    14761 attcctcagg aaggtttgtg agataaattt agactggcgg taaatcaaac agcaggattt
    14821 cactgtcgct atcagcatgg atggaaatcg cctgctcatc ccagattgcc agaccatcgc
    14881 tggtcgaggc tttcacgccg ttaatggtga cattgccttt caccacctgg atccagacgc
    14941 ggcgttcagc ggcaatctga tgcaccgact gctcatcttt caccagcgcc caacgataca
    15001 gttccatatc ctgatgcact ttcaatgaac catcacgcgc atccggcgaa agcaccagtt
    15061 gtttgccctg cacggcatcg aagcgacgct gttcataacg cggcgtaatg ccgttttctt
    15121 caggcatgat ccagatctga tacagatgca gacgctcggt gctgcttggg ttgtactctg
    15181 agtgacgaat acccgtaccc gcactcataa tctggaactc acccgccgga acctgctctt
    15241 tattgcccat gctgtcctga tgctcaacag taccttccag cacgtaggtc aaaatttcca
    15301 tatctttatg cgggtgagtg ccgaagccct gccctgcttc aatcacgtcg tcgttaatca
    15361 cgcgcagcgc ggagaagccc ataaagttcg gatcgtaata gttggcaaaa gagaaagtat
    15421 gccaggagtc cagccagcca tgatttgcat gaccacgttc atttgctttg cgtaagtaga
    15481 tcatttgtat tcgccccctg aatgatttcg atgggcttag tgtggacccg atccgcctgg
    15541 gatgatagag ggtgaaaatt gacccctctg ttcaaaaaaa ttgaactatt cgaggggcca
    15601 tcaaacttac ttcgcgagag tcactgtgga gggagaagct tgctcaaaac cgcgttcaag
    15661 gatttccaga ttggtaagaa cttcagattc cttgacgtaa tttggcgcac cgttggtgat
    15721 ggtttgatac aacgcatcat aaacgcgccc ataatcgccc atctctggct tcatctcttc
    15781 tcttaccgtc acgccctcgt cattgacata ctccagcaca ccgaccgaat catccgctgc
    15841 gaatcctggt tcgcccggca taatattagc cttcaggctg gtttcctgct ggtcgatacc
    15901 gtatttaata aacgaacctt tcttaccgtg aacgataaat ttcggataat cgattttcac
    15961 cagatggctg gttttgacga tggcttttag atcgccataa aacagctgcg cttcaaaggt
    16021 gtcgtccgga ttggctttat tacgcaggct gcggatgtca taagcgacgt gatccgggcg
    16081 accgaacaga gaaataatct ggtccatcgt atgcacacca aggccataga acgcgccatc
    16141 ctgcggcagc cccggtttgg tttctgccac cgggcggtaa tagtcaaaat ggctttccac
    16201 ttcgacaatc tctcccagct tgccgctttc aatcgctttt ttcgctgtca ggaagcagga
    16261 atcaaagcga cgattctgat acggcgtaac gatcagccct ttgcttttcg ccagtgcaaa
    16321 cagctctttc gcctgcgcaa ttgtcggagt gaacggtttt tcgaccagca catttttccc
    16381 ggcttccagc gcgcgcttcg cgtactcgaa gtggctgtcc gcgtgggtgc agacgacaac
    16441 cagcttaaca tcgggatcgt ttagcacttc gtcgagatcg ctggtgaaat ggatatggga
    16501 ataaatgggg gcctgttctt ccggcttcgc atggcggcga aaaatatgcg cgacatgcca
    16561 gctatccttg cggttaagta catacggcag atggtaacgg gtggtgcttt tgccgaagcc
    16621 aataaaggcg cagttaatga ccatgatttc gtccttttta aggtggttat ttacacctta
    16681 gcgcaaagcg gacgtggttc ctactggagt gcgcataacg aacacaagca cttcccgagg
    16741 ataaattgag aacgaaaggt caaaaaaaag ccagcacccg gctggctaag taatactgga
    16801 agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag ggtcttcctg atcgcgagac
    16861 aataataatc attctcattc gcacttgtcc aacacttttt gcaaaaaaat gcatttgact
    16921 cgcatttgaa agtcaatgat gttgaaaggg acatttaccc caaagaggac aaaggaatga
    16981 gtgagatagt aatacgccac gcagaaacac gcgattacga ggccatcagg cagattcacg
    17041 cccagccgga ggtgtattac aacacgctac aggtgcctca tccttcagaa caaatgtggc
    17101 tggaacgact caccgctcgc cctggcatca agcagttagt cgcctgtatt gatggaatcg
    17161 tcgtaggcca tctcaccatt gacgtgcaac aacgcccacg ccgcagtcat gttgccgatt
    17221 ttggtatctg tgtcgactcc cgttggaaga accgcggcgt cgccagcgcc ctgatgcgag
    17281 agatgattga aatgtgcgac aactggttgc gggtagatcg tattgaacta acggtgttcg
    17341 tcgataacgc gccagcaatt aaggtctata aaaaattcgg ctttgagatt gaagggactg
    17401 gcaaaaagta cgcattactt aatggtgaat atgtcgatgc gtattatatg gcgcgggtga
    17461 agtaagatag tgcccttttt ctgagatgga aaaagggtgt cattcaaaat
//
LOCUS       DADTFG010000103        17429 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.103, whole genome shotgun sequence.
ACCESSION   DADTFG010000103 DADTFG010000000
VERSION     DADTFG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.103"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(361..1557)
                     /gene="hemY"
                     /locus_tag="J1138_003193"
     CDS             complement(361..1557)
                     /gene="hemY"
                     /locus_tag="J1138_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBA8454956.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1560..2765)
                     /gene="hemX"
                     /locus_tag="J1138_003194"
     CDS             complement(1560..2765)
                     /gene="hemX"
                     /locus_tag="J1138_003194"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBA8454957.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     TPAAAPQGE"
     gene            complement(2787..3527)
                     /gene="hemD"
                     /locus_tag="J1138_003195"
     CDS             complement(2787..3527)
                     /gene="hemD"
                     /locus_tag="J1138_003195"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBA8454958.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCKCYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3524..4465)
                     /gene="hemC"
                     /locus_tag="J1138_003196"
     CDS             complement(3524..4465)
                     /gene="hemC"
                     /locus_tag="J1138_003196"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBA8454959.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNTYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGIALAEELLNNGAREILAEVYNGDAPA"
     gene            4852..7398
                     /gene="cyaA"
                     /locus_tag="J1138_003197"
     CDS             4852..7398
                     /gene="cyaA"
                     /locus_tag="J1138_003197"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBA8454960.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDSRLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(7438..7758)
                     /gene="cyaY"
                     /locus_tag="J1138_003198"
     CDS             complement(7438..7758)
                     /gene="cyaY"
                     /locus_tag="J1138_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBA8454961.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7806..8069)
                     /locus_tag="J1138_003199"
     CDS             complement(7806..8069)
                     /locus_tag="J1138_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454962.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQK
                     QGKGVVYLRPYEKTNLSLYRDIIIPVTDESCRNAAADGQTLKP"
     gene            8228..8431
                     /locus_tag="J1138_003200"
     CDS             8228..8431
                     /locus_tag="J1138_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA8454963.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8468..9292
                     /gene="dapF"
                     /locus_tag="J1138_003201"
     CDS             8468..9292
                     /gene="dapF"
                     /locus_tag="J1138_003201"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBA8454964.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9289..9996
                     /locus_tag="J1138_003202"
     CDS             9289..9996
                     /locus_tag="J1138_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBA8454965.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            9993..10889
                     /gene="xerC"
                     /locus_tag="J1138_003203"
     CDS             9993..10889
                     /gene="xerC"
                     /locus_tag="J1138_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBA8454966.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMSRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10889..11605
                     /gene="yigB"
                     /locus_tag="J1138_003204"
     CDS             10889..11605
                     /gene="yigB"
                     /locus_tag="J1138_003204"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBA8454967.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11689..13851
                     /gene="uvrD"
                     /locus_tag="J1138_003205"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11689..13851
                     /gene="uvrD"
                     /locus_tag="J1138_003205"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBA8454968.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13896..14798)
                     /locus_tag="J1138_003206"
     CDS             complement(13896..14798)
                     /locus_tag="J1138_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HBA8454969.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(14881..15645)
                     /locus_tag="J1138_003207"
     CDS             complement(14881..15645)
                     /locus_tag="J1138_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBA8454970.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            16015..16965
                     /gene="corA"
                     /locus_tag="J1138_003208"
     CDS             16015..16965
                     /gene="corA"
                     /locus_tag="J1138_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBA8454971.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17001..17429)
                     /locus_tag="J1138_003209"
     CDS             complement(17001..17429)
                     /locus_tag="J1138_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HBA8454972.1"
                     /translation="MRPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQDEIKS
                     QNPLPEDSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINE
                     YPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEKDNFQQ"
BASE COUNT         3972 a         4543 c         4650 g         4264 t
ORIGIN      
        1 attcactcag gaagttatta ctcaggaagc aaagaggatt acagaattat ctcataacaa
       61 gtgttaaggg atgttatttc ccggttctct gtggcataat aaacgagtag atgctcattc
      121 catctcttat gttcgcctta gtgcctcata aactccggaa tgacgcagag ccgtttacgg
      181 tgcttatcgt ccactgacag atgtcgctta tgcctcatca gacaccatgg acacaacgtt
      241 gagtgaagca cccacttgtt gtcatacaga cctgttttaa cgcctgctcc gtaataagag
      301 caggcgtttt tttatatatc agaaaggccc cggaggtgct tgcctccggg tgagaaagag
      361 ctactgtggc gggttgttct gcaacgttaa catcaaaccg tcgcgacgca tcgctgcggc
      421 ttcttccggc ttgtgcagtc tgtccagcgc gtcggcaagc catgcgtaat cgtaggcgtc
      481 cggacgttgt ttcagcgccg cgcggaaggc gagcgatgct tcctgccatt cgccgtgctt
      541 catcagcgac tgacccagtg tgctccacaa caacgggcga tcgcccacgt ttttgatctg
      601 ttggcgcagc actttttcca gctgttccgg gttattggtt ttcagacgcg ggatcggcag
      661 cagcaggcga tcgtcatact ggcgtttcag gccgtcgatg ataatttgct gggcggtgtc
      721 atggtcatca cactcaataa ggtgttccgc cattgccacc tgtaacgcca cctgatgacg
      781 cgttttccgg ctttggtttt tccaccagtt acgcaaacct tcgctaccgt tatcggcacg
      841 agcctgatcc atcaggccaa tccatgcctg ttgttccagc attgcacgat gttcttcatc
      901 accaacatgg gctttcgcca ttgatgggat gatatccagc agcgaactcc atgcacctgt
      961 gcggatatac gcctgttccg ccagacgtaa tacttccgga tggcgtggcg taacttccag
     1021 cagcttatcc acgccgtggc gtgcagcatg gttttcatta cgggccagtt gcagacgtac
     1081 acgggtgatt tctaccggaa ttgtgtcgtt gccggccagc tccgctgcgc gttccagatg
     1141 ttggttggcg cgtgcttcat caccgcgttg ttgcgcggct tcggcagcca gcagatagtt
     1201 caccaccggt tgttcagcgt gatcggcatt tttcgccatc agcttttcaa cttgctgata
     1261 atcgccttcc gccagtttca gcagcgcctg ttcggtctgc ttacgggcac ggcgacgctt
     1321 acgtccgaca aaccacccac gggtgtgcgc accagtgcgg aagatccgcc gcagtagcca
     1381 ctcaatggca aacaacacga ccatcgccag aatcaaaata atcgccaggc cagtgacgct
     1441 ggtttcgata ttgtagttat cggtctggat cagcacgtaa ccctgatgac cggcaatcat
     1501 cgggccaacc acgatccccg caatcagcaa aacaaagagc aacaacactt ttagcatggt
     1561 tattctcctt gcggcgcggc tgccggagta tcagcttgcg gtgcaggtgc aggtgcaggt
     1621 gcaggtgcag gtgcaggttt agcttccgtt gtccccgctg ccggttgtgc cagcaggtta
     1681 cgcacgcgag tctgcatcag tttttccagc atcgcctggc tttgcagggt ttccgggaga
     1741 tccatcgaga tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg
     1801 gcatcatcag tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga
     1861 taagtctctt cctgatgacg cggtacggct tgtgccgcga ccagcaggcg agagcgaata
     1921 ttttcgcgca gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga
     1981 cggcgaatcg taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc
     2041 cattcgctga tggaactgga aagctcttca ccgtcggaat ccatcggcga accatcgctg
     2101 tcattatcag ccagacgcag gttatctacc tgatttgaaa gctgattaag cttaaggata
     2161 atgccgtcat aatccacctg cgataccgca gaaaggctgg cgatatcatc ggtaattgcc
     2221 cggcgaacgg taatcagact cgggtcattc atatccgcca ggctggcgtc tgcacttttc
     2281 agcaacgctg cagcggtcgt gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc
     2341 accaggaaat cagcctgagc cagcagccag gttttagcat cgctgccgga aatagtcgcg
     2401 accttttgtt ggacttcatc cagctgtttt gccagcgttt cttgctgacg attcgcctgc
     2461 tcaagctgta cagcttgttg cttgataatg ccttccagct cggctttttg gctctcctgg
     2521 gctttttgca gtgccgtaag ttggttagcc agggcatcgc tggtggcagt ctgattgacg
     2581 gcctgttgtt taccccagcc atacaaaccg acgcccgccg ccagagcaat agcgatagcc
     2641 accgcgctga gaatcaatgc ggtattgttc ttactctttt tttctgttgc gacaggttgt
     2701 gacgtggtgt ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc
     2761 gtcattatgg cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca
     2821 gcgttatcgg cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc
     2881 tcactgacga ccaatagtcg acagcgtagt aaccagtgct cacgatacca ttgtgggatc
     2941 agcgaccaga gttgctgcaa catttcaccg ctggtaacaa cgaccgtcgt cacctcgcgg
     3001 gattgccagc gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat
     3061 ttacaaaaag tgacctcagc accgcgcgcc gtcagggtat ccccaattag ctcacgaccg
     3121 ccattgccac gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt
     3181 agcaagactt cgctgatttc ccgatcctgc gggtagagaa tcttctgtcc gcttacggta
     3241 tgtagtgcca gtgcggtggt gcgtccaatg gcgaaataag taggtagtcg gggccattta
     3301 cgatcttgct gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac
     3361 aacagatcgc tctctcccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc
     3421 ggagaaaact caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc
     3481 actaactctt ctcctgcggg agacgggcgg gtgaccagga tactcatgcc ggggcgtctc
     3541 cgttatagac ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg
     3601 caatccccat ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatctgcg
     3661 aaccgtccgg cgcgccgacc aacgcacgca gccagatttc gccatcaata agctcggcgt
     3721 agctaccaat tggcacctga catccgcctt cgagacgggt attcatggcg cgttcggcgg
     3781 taacgcgcag tgcagtttcg tggtgattca gcgcggcgag cagctcgcga gtgcgtgcat
     3841 catcaaggcg gcattcaatg cccaccgcac cttgccctac cgccggaaga gaaatctcgg
     3901 gtggcaacgc ggcgcgaatg cgggactcca gacctaaacg ttttagtccg gctacggcaa
     3961 gaatgatggc atcatattcg ccgttatcca gtttgctcag acgagtgccg acgttgccgc
     4021 gcagggagcg gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac
     4081 tggacgtccc gacgatactg cctgccggta gcgcatccag actgtcatag gtgttggata
     4141 caaaggcatc gcggggatct tcgcgctcgc aaatggtgac cagccccaga ccttgcggga
     4201 attcaacggg cacatctttc attgaatgaa cggcaatatc ggcgcgattt tccaggagcg
     4261 cgacttccag ctctttcaca aataagcctt tcccgcctac tttcgccagc ggcgtatcaa
     4321 gaatcacatc gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg
     4381 ccatcaactt gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg
     4441 caattcttaa aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct
     4501 aacatccttg ccagagcgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt
     4561 aaaatgaggt ctggcagtgg atcctgacag gcgctttgag ccttcgtaat aaggaattta
     4621 cagagaataa acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa
     4681 ggtgttaaat tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg
     4741 cgaaaagtgg taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt
     4801 tgatgttggc ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc
     4861 tatattgaga ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg
     4921 cttgctgcta tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac
     4981 tatcaccatc cgctaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt
     5041 tacacgcctg atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg
     5101 tcagtacagg acccaccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc
     5161 acctcgtccg tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc
     5221 tggctcgata gcgaagagcg ccagttgcta caacgtaaat gtagtctgct ggaaagctgg
     5281 gccgcctcgc tgggtgtgga agtcagcttc ttcctgattg atgaaaaccg cttccgtcat
     5341 aatgaaagcg gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt
     5401 gacgaatttt atcgtaccgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg
     5461 ccgtgcgacg aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg
     5521 ctgacgccaa atgaatggct ggatcttggt ggcttaagct cgctttctgc tgaagagtac
     5581 tttggtgcca gcctttggca gctctacaag agtatcgatt ccccatacaa agcggtgctg
     5641 aaaacactgc tgctggaagc ctattcctgg gaatacccga acccacgtct gctggcgaaa
     5701 gatatcaaac agcgtttgca cgacggcgag attgtatcgt ttggcctcga tccatactgc
     5761 atgatgctgg agcgtgttac tgaatatctg acggctattg aagatttcac ccgtctggat
     5821 ttagttcgcc gctgcttcta tttaaaagtg tgtgaaaaac ttagccgtga acgcgcctgc
     5881 gtaggctggc gtcgtgaagt gttaagccag ttggtgaaag agtgggaatg ggacgattct
     5941 cgtctggcaa tgcttgataa ccgcgctaac tggaagatag atcaggtgcg tgaggcgcac
     6001 aacgagttgc tcgacgcgat gatgcagagc taccgcaatc tgatccgctt tgcgcgtcgc
     6061 aataacctta gcgtttccgc cagcccgcag gatatcggtg ttctgacacg taaactgtac
     6121 gccgcgtttg aagcgttacc gggtaaagtg acgctggtaa acccgcagat ttcacccgat
     6181 ctctcggaac cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt
     6241 tggtatctgt ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa
     6301 tataaccgtt acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctct
     6361 cgcacccgtt tgtatattaa aggtaacggc attgtcgatt tgcctaagtt gcaggagatg
     6421 gtcgcagacg tgtcgcacca tttcccgctg cgcttacctg caccgacacc gaaggcgctc
     6481 tacagcccgt gtgagatccg ccatctggcg attatcgtta acctggaata tgacccgacg
     6541 gcagccttcc gcaatcaggt ggtgcatttt gatttccgta agctggatgt cttcagcttt
     6601 ggcgagaatc aaaattgcct ggtgggtagc gttgacctgc tgtatcgcaa ctcgtggaac
     6661 gaagtgcgta cgctgcactt caacggtgag cagtcgatga ttgaagccct gaaaaccatt
     6721 ctcggcaaaa tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgctacagc
     6781 cagcatctgc gcggcttaat ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa
     6841 ctgcgtcttt ccagcacccg ccaggaaacc gggcgcttca aggcgctgcg cgtctccggt
     6901 caaacctggg gcttgttctt cgaacggctg aatgtatcgg tacagaaact ggaaaacgcc
     6961 atcgagtttt atggcgctat ttcgcataac aaactgcacg gcctgtcagt gcaggttgaa
     7021 accaatcatg tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag
     7081 ttcttcttcg aagaaacgca agacgagaat ggctttaata tctacattct cgacgaaagc
     7141 aaccgggttg aggtgtatca ccactgcgaa ggcagcaaag aggaattggt gcgtgacgtc
     7201 agtcgcttct actcgtcttc gcacgaccgt ttcacctacg gctcaagctt tatcaacttt
     7261 aacttgccgc agttctatca gattgtgaag gttgatggtc gtgaacaggt gattccgttt
     7321 cgcaccaaat ctatcggtaa catgccacct gccaatcagg agcatgacgc gccgctgttg
     7381 cagcagtatt tttcttgatg ctaatgccgg aaagcgaggc ttatccggca ttagctctta
     7441 acgaaaactg acggtttctc ccgcctgctg cgtcgccgcc tgttccagca aatcccagaa
     7501 ggtttcaccg ctacgatcgc aaatccactc gtcgcctttc agatcaaaat ggtagccacc
     7561 ctgtttggtt gccagccaca cctggtgcag tggttcctgg cggttgataa tgattttgct
     7621 gccgttctca aaggtaatgg ttagtacacc gccgttgatt tcacagtcga tatcgctgtc
     7681 gccatcccag tcgtccagat gttcttcaat ggtcagccac agttgatcag ccaggcgatg
     7741 aaattcactg tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca
     7801 tggggtcagg gcttcaaagt ttgcccgtct gctgctgcgt tccggcatga ttcatccgtc
     7861 accggaataa taatatctct atatagcgaa agattggtct tttcataagg gcgcaggtac
     7921 accacgcctt ttccctgttt ctggtaaata ttatgttccg gctcatcaag ccgacatccc
     7981 catgccgacg ataagaacaa cgaacaagac aataaacaaa ccacattgcg atagtgcata
     8041 aagccatcct ggcgcgaggt gccgatcacg aaactaccag caaaacataa atcgccacga
     8101 gtaagcgtta tactcgcagc atttcctcac ttttcagact tcataaagag tcgctaaacg
     8161 cttgctttta cgccttctcc tgcgatgata gaaagcagaa agcgatgaac tttacaggca
     8221 atccataatg aaaaacgtgt ttaaggcact cactgtatta cttactctct tcagcctgac
     8281 gggctgcggt ctgaaaggtc cgctctattt cccgcctgca gataaaaacg caccgccgcc
     8341 gaccaaaccg gtagagacgc aaacgcaatc cacggtgccg gataaaaacg accgcgccac
     8401 tggcgatggt ccatcccagg tgaattacta aaagtcagtt tctgtacccg cgtgattgga
     8461 gtaaatgatg cagttctcga aaatgcatgg ccttggcaac gattttatgg tcgtcgacgc
     8521 ggtaacgcag aatgtctttt tttcaccgga gctgattcgt cgcctggcgg atcggcacct
     8581 gggggtaggg tttgaccaat tgctggtggt tgagccgccg tatgatcctg aactggattt
     8641 tcactatcgc attttcaatg ctgatggcag tgaagtggcg cagtgcggca acggcgcgcg
     8701 ctgctttgcc cgttttgtgc gcctgaaagg gctgaccaat aagcgcgata tccgcgtcag
     8761 caccgccaac gggcggatgg ttctgaccgt caccgatgat gatctggtcc gcgtaaatat
     8821 gggcgaaccc aacttcgaac cttccgccgt gccgtttcgc gctaacaaag cggaaaagac
     8881 ctatattatg cgcgccgccg agcagacaat cttatgcggc gtggtgtcga tgggaaatcc
     8941 gcattgcgtg attcaggtcg atgatgtcga taccgcggcg gtagaaacgc ttggtcctgt
     9001 tctggaaagc cacgagcgtt ttccggagcg cgccaatatc ggttttatgc aagtggttaa
     9061 gcgcgagcat attcgtttac gcgtttatga gcgtggggca ggagaaaccc aggcctgcgg
     9121 cagcggcgcg tgtgcggcgg tggcggtagg gattcagcaa ggtttgctgg ccgaagaagt
     9181 acgcgtggaa ctccctggcg gtcgtcttga tatcgcctgg aaaggtccgg gtcacccgtt
     9241 atatatgact ggcccggcgg tacatgtcta cgacggattt attcatctat gaagcaacca
     9301 ggggaagaac tgcaggaaac actcacggag cttgatgacc gggcggttgt cgattatctg
     9361 attaaaaatc ctgagttttt tatccgcaat gcgcgcgcag tagaagcgat acgtgtgccg
     9421 catccggtac gcggcaccgt ttcgttggtc gagtggcaca tggcccgcgc acgtaatcat
     9481 attcatgttc tggaagagaa catggcgttg ttgatggaac aggctatcgc taacgaaggc
     9541 ctgttttatc gtctactcta cctgcagcgc agtctcaccg ccgccagtag tctcgacgat
     9601 atgctgatgc gtttccatcg ctgggcgcgt gatttagggc tggcaggtgc gagtctgcgc
     9661 ctgtttccgg atcgctggcg cttaggtgcg ccgtcgaacc acactcatct ggcattaagc
     9721 cgtcagtctt tcgaaccgct gcgtattcag cgtctggggc aggaacagca ctatctgggg
     9781 ccgcttaacg gaccggagct tctggtggtg ctaccggaag cgaaagcggt gggatcggtg
     9841 gcgatgtcga tgctgggaag cgatgctgat ttgggggttg tgctgtttac cagtcgcgat
     9901 gccagtcact atcaacaagg gcagggaacg cagttacttc atgaaattgc gctgatgttg
     9961 ccggagcttc tggcgcgttg gattgaacgc gtatgaccga tttacacacc gatgtagaac
    10021 gctacctacg ttatctgagc gtggagcgcc agcttagccc gataaccctg cttaactacc
    10081 agcgacagct tgaggcgatc atccattttg ccagcgaaaa cggcctgcaa agctggcagc
    10141 aatgtgatgt gacgatggtg cgtaattttg ctgtacgcag tcgccgtaaa gggctgggag
    10201 ctgcaagtct ggcgttacgg ctttctgcgc tacgtagctt ttttgactgg ctggtcagcc
    10261 agaatgaact caaagctaac ccggcgaaag gtgtttcggc accgaaagcg ccgcgtcatc
    10321 tgccgaaaaa catcgacgtc gatgatatga gtcggctgct ggatattgat atcaacgatc
    10381 ccctcgccgt gcgcgatcgg gcaatgctgg aagtgatgta cggcgcgggt ctgcgtcttt
    10441 ccgagctggt ggggctcgat atcaaacacc tcgacctgga gtctggcgaa gtgtgggtga
    10501 tgggaaaagg cagcaaagag cgccgcctgc cgattggtcg caacgccgtg gcgtggattg
    10561 agcactggct tgatttgcgc gacctgtttg gtagtgaaga cgacgcgctt ttcctgtcga
    10621 aactgggcaa acgcatctcc gcgcgtaatg tgcagaaacg ctttgccgaa tggggcataa
    10681 aacaagggct gaataatcac gttcacccgc ataaattacg tcactcgttc gctacccata
    10741 tgctggagtc gagcggcgat cttcgtggtg tgcaggagct gctgggtcat gccaacctct
    10801 ccaccacgca aatctatact catcttgatt ttcaacacct tgcctcggtg tacgatgcgg
    10861 cgcatccacg cgccaaacgg gggaaataat gcgtttttac cggcctttgg ggcgcatctc
    10921 ggcgctcacc tttgacctgg atgataccct ttacgataac cgtccggtga ttttgcgcac
    10981 cgagcgagag gcgcttacct ttgtgcaaaa ttatcatccg gcgctgcgca gcttccagaa
    11041 tgaagatctg caacgcctgc gccaggcggt acgggaagcg gaacctgaga tttatcacga
    11101 cgtgacgcgc tggcgttttc gttcgattga acaagcgatg ctcgacgccg ggctgagtgc
    11161 cgaagaagcc agtgcaggcg cacatgcagc aatgatcaac tttgccaaat ggcgcagccg
    11221 gatcgacgtc ccgcagcaaa cccacgacac cttaaaacag ctggcgaaga aatggccgct
    11281 ggtggcgatc accaacggta acgcccagcc ggaactgttt ggtttggggg attattttga
    11341 gtttgtgctg cgcgctggcc cgcacgggcg ctcaaaaccg ttcagcgata tgtacttcct
    11401 ggcggcggaa aaactcaacg tgccgatcgg cgagatctta catgtcgggg acgatctcac
    11461 cactgacgtg ggtggggcaa ttcgcagcgg aatgcaggcc tgttggatca gaccggaaaa
    11521 tggcgatctg atgcaaacct gggacagccg tttactgccg catctggaaa tttcccggtt
    11581 ggcatctctg acctcgctga tataatcagc aaatctgtat atatacccag ctttttggcg
    11641 gagggcgttg cgcttctccg cccaacctat ttttacgcgg cggtgccaat ggacgtttct
    11701 tacctgctcg acagccttaa tgacaaacag cgcgaagcgg tggccgcgcc acgcagcaac
    11761 cttctggtgc tggcgggcgc gggcagtggt aagacgcgcg tactggtgca tcgtatcgcc
    11821 tggttgatga gcgtggaaaa ctgctcgccg tattcgatta tggcggtgac gtttaccaac
    11881 aaagcggcgg cggagatgcg tcatcgtatc gggcaactga tgggcactag ccagggcggt
    11941 atgtgggtcg gcaccttcca cgggctggcg caccgcctgc tgcgtgcgca ccatatggac
    12001 gccaacctgc cgcaggattt ccagatcctc gacagtgaag accagctacg tctgcttaag
    12061 cgtttgatca aggcgatgaa tctcgacgag aagcagtggc cgccgcgtca ggcaatgtgg
    12121 tacatcaaca gccagaaaga tgaggggctg cgtccgcatc atattcagag ctacggtaat
    12181 ccggtggagc agacctggca gaaggtgtat caggcgtatc aggaagcgtg tgaccgcgcg
    12241 ggcctggtgg acttcgccga gctgctgcta cgtgctcacg agttgtggct taacaagccg
    12301 catatccttc agcactatcg cgaacggttt accaatatcc tggtggacga attccaggat
    12361 accaacaaca ttcagtatgc gtggatccgc ctgctggcgg gcgataccgg caaagtgatg
    12421 atcgttggcg atgacgatca gtcaatctac ggctggcgcg gagcgcaggt cgagaatatt
    12481 cagcgtttcc tcaatgattt ccccggtgct gaaactatcc gtctggagca gaactaccgc
    12541 tctaccagca atattctgag cgccgctaac gccctgattg aaaacaataa cgggcgtctg
    12601 ggtaaaaaac tgtggaccga tggcgcggac ggtgagccta tttccctcta ttgcgctttt
    12661 aacgaactcg acgaagcgcg ttttgtggtt aaccgcatca aaacctggca ggacaacggc
    12721 ggggcgctcg ctgagtgcgc cattctctac cgcagcaacg cccagtcgcg tgtgctggaa
    12781 gaggcgttat tgcaggcgag tatgccgtat cgtatttacg gcgggatgcg cttcttcgaa
    12841 cgccaggaaa tcaaagatgc gctctcgtat ctgcgcctga ttgccaaccg caacgacgac
    12901 gcggcctttg agcgtgtggt gaatacgcca acgcggggta ttggtgaccg gacgctggac
    12961 gtggtacgtc agacatcgcg agatcgccag ttaacactct ggcaggcatg tcgtgaactg
    13021 ttgcaggaaa aagccctcgc cggacgtgct gccagcgcct tacagcggtt tatggagctg
    13081 atcgacgcct tagcgcagga aactgccgat atgccgctgc atgtacagac tgaccgggta
    13141 attaaagact ccggcctgcg caccatgtac gagcaggaga agggcgaaaa aggtcagacg
    13201 cgtatcgaaa acttagagga actggtgacg gcaacgcgcc agttcagcta caacgaagaa
    13261 gacgaagatt taatgccgct gcaggcattc ctctcccatg cggcgctgga agcgggcgag
    13321 gggcaggcgg atacctggca ggatgcggtg cagttgatga cgctacactc ggcgaaaggt
    13381 ctggaattcc cgcaggtgtt tatcgttggt atggaagagg gcatgttccc aagccagatg
    13441 tcgctggatg aaggcgggcg tctggaagaa gaacgccgtc tggcctacgt tggcgtaacc
    13501 cgcgcgatgc agaaactgac gctgacctac gcggaaaccc gccgtctgta tggcaaagag
    13561 gtttaccatc gcccgtcgcg ctttatcggt gagttgccgg aagagtgtgt ggaagaggtg
    13621 cgcctgcgcg ccacggtaag ccgcccggtc agccatcagc gtatgggtac gccgatggtc
    13681 gagaacgaca gcggctataa gctcggccag cgcgtacgcc acgctaagtt tggtgaaggc
    13741 accatcgtca atatggaagg cagcggcgag catagccgtt tgcaggtggc attccagggc
    13801 cagggtatta aatggctggt ggcggcttac gcccggttgg agacggtgtg acgttgcgga
    13861 atgcggtgcg ccataccgca ttccgcatca gcaccttact tagcgcactt cttcacatca
    13921 atattccagt cagcaccaga tgcaacctga tgtttctgcg cgaaattatc catattcagc
    13981 gccacggaaa cattcaataa gctgttcaga tacaccatcg gctggccttc cggcacatcg
    14041 ccgaagcttg cgacatacgg cattttccct gcgtattttt gctgggaacc ttcggaaatg
    14101 gtcacacaca acgtgtcgtt gagtttgatt cctgcctgat tcagcaactc gtcgctgata
    14161 ttgctccaga cgttgccgta ctgaatatcg agaatcggga tattgccctt cacttcacct
    14221 tttgttgctt tggctttctg ataagaaagt tcgacaactt ttgcaggaag ctctggcccc
    14281 acctgctcga acgtgatcgc cccggaagcc agacgtgcac cggtgtaggc atacacgtca
    14341 cgaccgtgga aggtatatga cttttcggaa cctttcagac ggttggtttt ttcatcgatt
    14401 tgccggacgc tctcaatacc cagcgattcc gccaccaacg taagcgtacc gttatctggt
    14461 gaaacgaagt actgaccgtt tttggttttc agcactaccg atttacggtc tgtaccaaca
    14521 cctggatcga ccacgctaac aaacaccgaa ccttgcggcc agtaactggc agtctgatac
    14581 aggcggtaag cgccttccca gatgttatat ggtggaattt cgtgtgttaa atcaaatatt
    14641 ttaagattat ggtcaacgcc gaaagcgacc cctttcatcg ccgagacggc gccatctttc
    14701 aggctaaaat ccgtttgtaa aatcaaagcg ttatccgcat aagcggatga tgaccccaga
    14761 caggttaata aaaataatgt tgcgagcgtt tttttcattt ttgtttccct tcatttatct
    14821 gatgattttc cagctgtgta gccagcccca gtttgtctgg cctcctcgag tgtacaatgt
    14881 ttaacctttc tgctcgacgg aaatcatggt gacaaaagga tagcgttgcc acgggattgc
    14941 ctcgcctttc atatacatat gtgaaattgt gccatcaaga taaagcagct gctcaacgtt
    15001 cagtttcgct ttggcataac aggcaaaatc ataaaaattt gtcgcctgct ggctcaacaa
    15061 aaacacggcg ttcccatgtt tattaatccc aacaccgtta cgaattttac gtgaggcgac
    15121 gttgggatga atacgcggat taattacacc gttttccatc aacaatggcc ctgactgcac
    15181 cgcaaactga atctctttac tggttttaaa ggcatccaga cgaacgatgc cgactttatc
    15241 tcccgcgaca taaaacactc cgccaggacg aataaagaaa ttcccttcac ctgaagcgag
    15301 atttaacgcc accttctgct gaccgttttc gatgtacaaa ccgagcggcg catagctttc
    15361 atcatagatg ccgccgttca tcgccatctg cacctgaccc tgactattaa tatccgccag
    15421 cagagcatgt aacgttcccc acgcttcgcc attggctttt tgccagtaca ttttcacccg
    15481 ctctgtttga ggattaacgg tatacgcctg tacggtcagc gtcggatctg agggtgcgca
    15541 atcatcagcg gcaacagcaa acaagggaag taaggtgagg gtgagaaaaa tccgtttgag
    15601 attcaaggtg atcattcctt taccaatgaa tagccgatgc gccattatag gccctgaatg
    15661 tgggattttt ttatcctgtt agcgaccttg acgagtacca aaaagcgcga agttcaacta
    15721 ttgttctgtg gtgttctgtt gcgtgttgac ggcaaaattt tgctggcgta acatgcgcgc
    15781 acgatcactc taagaggaca ttcgccttgg acacacccag tagatactgg ctcattatcc
    15841 tgtcatccag gatcaactcc taaggctatc cctttttgct gatagcctta gcggttgtca
    15901 gcgacctcaa tttttcccgt cgcgctgagt caggctgttt aatggtctga aacccaattt
    15961 gtttctgtgt gcccaccgaa ctgtccgata ttttaagcat tgggagtccc ggtcatgctg
    16021 agcgcatttc aactggaaaa taaccgactg acccggctgg aagtcgaaga gtcacaaccc
    16081 cttgtaaatg cagtatggat tgatcttgtc gaaccggacg acgacgagcg actgcgcgta
    16141 caatctgaac ttggccagag cctggcaacc cgcccggaac tggaagacat cgaagcatcg
    16201 gcacgtttct ttgaagacga tgacggcctg catattcact ccttcttctt ctttgaagat
    16261 gcggaagatc acgccggtaa ctccactgtg gcatttacca tccgtgatgg tcgtctgttt
    16321 actctgcgtg agcgtgaact gcccgctttt cgcctttacc gtatgcgtgc ccgtagccag
    16381 tcaatggtgg acggtaacgc ctacgagctg ctgctggatc tgttcgaaac caaaatcgaa
    16441 cagttggcag atgaaattga aaatatctac agcgacctgg agcagttgag ccgggtaatt
    16501 atggaagggc atcagggcga tgagtatgac gaggcgctct ccacactggc ggaactggaa
    16561 gatatcggct ggaaagttcg cctgtgtctg atggataccc agcgtgcgct gaacttcctg
    16621 gtgcgtaagg cgcgtttacc gggcgggcaa ctggagcagg cgcgtgaaat cctgcgagat
    16681 atcgaatccc tgctgccgca taacgaatcc ctgttccaga aggtgaactt cctgatgcag
    16741 gcggcgatgg gctttatcaa tatcgagcag aaccgcatca tcaaaatctt ctcggtggta
    16801 tccgtggtgt tcctgccacc gacgctggtc gcttccagct acgggatgaa ctttgagttt
    16861 atgccggaac tgaagtggag cttcggctac ccaggcgcga ttatctttat gatcctcgcg
    16921 ggcctggcac cgtatctgta ctttaagcgg aagaactggt tgtaatataa atgccgggta
    16981 aagattgttc tttatccggc ttattgctga aaattgtctt tttcaaagtc atgcgggttg
    17041 aacactcgct ctatcttatt ttcctgggtt agttgatact cattgttatc agcaataaaa
    17101 aaatgagtat tacggtaagg atattcatta ataaccatta aatagttttt tacgatgcaa
    17161 ttatcattgc tcttaatcgt cgggaaacag tagagatctt catacggccc ttcgatatat
    17221 tttttatagt cagtgaagcc attaccgata tcaataacat accaggtatg ccaactatct
    17281 tctggtaggg gattttgact tttaatttca tcctgatgct tcagatacca atttatttta
    17341 tcgctatcag tccatggcag atgatcaacg accagaaaga ctgaattgcc agcatcagag
    17401 tatgtataca aaatctgtac aggacgcaa
//
LOCUS       DADTFG010000104        17419 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.104, whole genome shotgun sequence.
ACCESSION   DADTFG010000104 DADTFG010000000
VERSION     DADTFG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.104"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            101..619
                     /locus_tag="J1138_003210"
     CDS             101..619
                     /locus_tag="J1138_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBA8454973.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            635..1174
                     /locus_tag="J1138_003211"
     CDS             635..1174
                     /locus_tag="J1138_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454974.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1267..2844)
                     /gene="guaA"
                     /locus_tag="J1138_003212"
     CDS             complement(1267..2844)
                     /gene="guaA"
                     /locus_tag="J1138_003212"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBA8454975.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2913..4379)
                     /gene="guaB"
                     /locus_tag="J1138_003213"
     CDS             complement(2913..4379)
                     /gene="guaB"
                     /locus_tag="J1138_003213"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBA8454976.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4541..5917
                     /gene="xseA"
                     /locus_tag="J1138_003214"
     CDS             4541..5917
                     /gene="xseA"
                     /locus_tag="J1138_003214"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBA8454977.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVQQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLITRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5986..14037)
                     /locus_tag="J1138_003215"
     CDS             complement(5986..14037)
                     /locus_tag="J1138_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HBA8454978.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPTKTHEVAFTAKPSEFNADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPAVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLVAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKMTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQASETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEGGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNFYWSSLAGMSGQHHAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGINR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEATLTLTQPGGVGVKTHITAR
                     MRSDFTASDEKDVIFTVITSPDTDKARMWGHMLGIIEANNIFKRPRLADETDNEQGSV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANAIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAKIAATTDRDVSAGSRAQAKVG
                     DTITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQSQGVGLSTPLNIVPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVFSPDGTLTENNEVWSRVSQANASSTSKG
                     GCGANMLPRRSQLSALYDANNGNGVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDIPFTLKRGDGYTRSEEKHVAGSGDALVAPVVVNGGLADETSLNNTAAAYS
                     AITGSDGTKILTITRPDTHGTKTSLTATLYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGGE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDRMNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSVGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQLTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEYPAGQSTLANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSGGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMSVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGNVV
                     AMDDLVVQPLSGSAVTLSESGNTISGMTGADGTASFSLRQDNTPGYKMPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNSKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENTPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14158..15135)
                     /locus_tag="J1138_003216"
     CDS             complement(14158..15135)
                     /locus_tag="J1138_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454979.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELVTKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15190..17367)
                     /gene="sinH"
                     /locus_tag="J1138_003217"
     CDS             complement(15190..17367)
                     /gene="sinH"
                     /locus_tag="J1138_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HBA8454980.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDKYLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEVALFGTDSLEKDPFAVTLGVKYQPV
                     PLIVVGTDFKAGTGDNTDLSVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVILWLKADTTNEHPECVIKDTPEEAIGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNIYRFVLWRDNNKDGVFQQVEKLTDEEMVQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4108 a         4603 c         4327 g         4381 t
ORIGIN      
        1 tttaataaat ttaatctatt cctttatacg caatacattt actttcctct tttgatgatc
       61 ttaaatgtct tatttttcgt aatgtgcata acaaggaata gtgatgaaat ttaaaaaatg
      121 tcttctgcct gtggcaatgt tagcgtcatt cactctggca ggatgccagt caaatgctga
      181 tgatcatgcc gccgatgttt atcaaaccga tcaactgaat accaaacaag aaactaaaac
      241 cgttaatatt atttccattc ttcccgcaaa agttgccgta gacaacgccc aaaataaacg
      301 aaatgcacaa gccttcggcg cgcttattgg tgcagtcgct ggcggtgtaa tcggccacaa
      361 cgtggggtct ggcagcaatt ccggaacgac ggcaggtgca gttggcggcg gagctgttgg
      421 cgcggcagcg ggttctatgg tgaatgataa aaccttagtg gaaggtgttt ctctaacgta
      481 taaggaaggc accaaagtgt atacctccac ccaggtgggt aaagagtgcc agtttacgac
      541 aggtttagcc gttgttatta ccacgacgta taacgaaacg cgtattcagc caaatacgaa
      601 atgtcctgaa aagagctaat gatcaggagg agtcatgaag aaagtttttc tttgcgccat
      661 cttagcctcc ttaagctatc cggctatcgc ctcatcattg caggatcaac tctcggctgt
      721 cgcagaagcg gaacagcaag gtaaaaatga agagcaaagg cagcatgacg aatgggtcgc
      781 ggagcgcaac agggaaatcc agcaagagaa gcaacgtcgc gcaaacgccc aggccgcggc
      841 taataaaaga gcggcaacgg cagcggcgaa taagaaagct cgtcaggata aactggacgc
      901 cgaagccact gcggacaaaa aacgcgatca aagttatgaa gatgagctac gcagcttaga
      961 gattcagaag caaaaactgg cgctggcgaa agaagaagcc cgcgttaagc gagaaaacga
     1021 atttatcgat caggaactga agcacaaagc tgcgcaaacc gatgtggtgc aatctgaagc
     1081 tgacgcaaac aggaatatga ctgaaggcgg tcgcgatctg atgaaaagcg tgggcaaagc
     1141 agaagagaat aaatcagata gctggtttaa ttaatcgatg ttagtaactt caatcctatg
     1201 attcgtgagt ataaaaaacc ctctgtagta acagagggtt ttgttcattc atagtgcaga
     1261 gtcaaatcat tcccactcaa tagtcgctgg tggcttgccg ctgatgtcat acaccacgcg
     1321 ggaaatacca ttcacttcat tgataatgcg gttggaaacg cgaccgagga aatcgtacgg
     1381 cagatgtgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca gagagacaac
     1441 ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtactggca ggaagacggt
     1501 gaacgcctgg ctgactttgt cgtacagatc cgctttacgc agttcttcaa tgaagatggc
     1561 gtcagcacgg cgcagcaggt cacaatactc tttcttcact tcccccagaa cacgaactcc
     1621 aaggcctggt cccgggaacg ggtgacggta caacatgtca tacggtaggc ccagctccag
     1681 accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca ggcccatctt
     1741 catctctttc ggcagcccac ccacgttgtg gtgagatttg atgacgtgtg ctttaccggt
     1801 tgcagaagca gcagattcga taacgtcagg gtagatggtg ccctgcgcca gccacttcac
     1861 gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac cgatgatttt
     1921 acgttttgct tccggatcgt tttcgccagc cagcgctgat aagaagcgat cttctgccgg
     1981 tacgtgaaca atattcagac caaagtgatc gccaaacata tccagaacct gctctgcttc
     2041 gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac cgatagcgcg
     2101 gtgcaacagc attgcggtaa cggaggaatc cacgccacca gagaggccga ggatgacttt
     2161 atcgtcgccc acttgctcgc ggatgcgggc tacagcgtcg tcgataattt tcgctggcgt
     2221 ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc gcataccctg
     2281 gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt cttcgttggc
     2341 catgatggca aacgggcagc tttcggtgct agctacggtg acgaagtcgg acgggatagc
     2401 ggtaacttta tcgccgtggc tcatccagac gtccagcagc ggtttaccat ctgcggtcag
     2461 cgcatcttcg ataccgcgaa ccagtgcgct gtcgtttacg acttcaacct gcgcgtagcc
     2521 aaattcacgt tcattagaag cctcaacgtg accgcccaac tgcatcgcca tggtctgcat
     2581 accatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg gcgcacgcgg
     2641 gctgttttct tcggtggtgc tttccgggcc gccggaaaga ataatgccgc ttggattgaa
     2701 gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa cacccagctc
     2761 acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa tgaggatgcg
     2821 atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa cagataatat
     2881 aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta gttcggggac
     2941 tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tacccgcacc gctgatacgt
     3001 acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag gcccatacag
     3061 gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt ataggctacg
     3121 cggccttcga taccttccgg caccagtttg tcggcagcgt tgtcgctctg gaagtaacgg
     3181 tcagaagaac ctttggacat cgcgcccagg gaacccatac cacggtaaga tttgtaagaa
     3241 cggccctggt agagttcgat ttcacccgga gattcttcag tacctgccag catggaacct
     3301 accatcaccg cgcttgcgcc agcggcgata gctttagcga tatcgcctga gaaacggata
     3361 ccgccgtcag cgataaccgg aatgccggta ccttccaggg cttctactgc atcagcaacg
     3421 gcggtgatct gcggaacacc gacaccagtc acgatacgcg tcgtacagat agaaccaggg
     3481 ccgataccca ctttaacggc actgcaaccg gcttctgcca gcgcgcgggc acctgcagca
     3541 gttgccacgt tgccgccgat gatttgcagg tcaggatatt tagcgcgagt ttcacggata
     3601 cgctgcagaa caccttcaga gtgaccgtga gaggagtcga tcagcagaac gtcaacgcct
     3661 gcggcaacca gcgcatcaac acgctcttcg ttaccagcgc ccgcgccaac cgctgcacct
     3721 acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc tttctggaag
     3781 tctttcacgg tgatcatgcc gatcaggtgg aattcatcat caaccaccag cgccttctca
     3841 acgcgttttt cgtgcatttt agccagtacc acttcacggg cttcaccttc gcgcacggtc
     3901 accagacgct ctttcggtgt catgtaaacg ctaaccggct ggctcaagtc ggtcacaaaa
     3961 cgcacgtcac gaccggtgat aatgccgacc agttcgtttt cttcggtaac aaccgggtag
     4021 ccagcaaagc cgttacgctc ggtcagttct ttcacttcac gcagcgtcgt ggttggcagt
     4081 acggtctgcg gatcggttac cacgccagat tcgtgttttt tcacacggcg aacttcttct
     4141 gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc ctgagccaga
     4201 gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat agggatattc
     4261 agacgaatag ttttcgtcag ctgagtgctg aggtcagcag tattcggcag aacggtagaa
     4321 tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat acgtagcatg
     4381 ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca gagcgtaacc
     4441 gattgcatct accccttttt gcaaaaaatg cttgctatcc ccggagggcg ggttactatc
     4501 gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt ctcaatcccc
     4561 tgcaattttt accgttagcc gcctgaatca aacggttcgt ctgctgcttg agcatgagat
     4621 ggggcaggtg tggatcagcg gcgaaatctc taattttaca caaccagcct ccggtcactg
     4681 gtactttacg cttaaagacg ataccgcgca ggtgcgctgt gcgatgttcc gaaacagcaa
     4741 ccgccgggtc accttccgcc cacagcacgg gcaacaggtt ttagttcgcg ccaatattac
     4801 actctacgag ccccgcggcg attatcaaat tatcgtcgaa agtatgcagc cggccggtga
     4861 aggattgctg caacagaagt acgaacagct taaagcgaag ttgcaggctg aaggtttgtt
     4921 cgatctgcaa tacaaaaaat cactcccctc ccctgcgcat tgcgttggtg tgatcacctc
     4981 aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg atccttctct
     5041 gccagtgatt atttatccca ccgccgtaca gggggatgat gcgccggggc aaatcgttcg
     5101 tgccattgaa ctggcgaata agcgcaacga gtgtgacgtg ttgatcgttg gccgtggcgg
     5161 tggttcgctg gaagatttat ggagttttaa tgatgagcgt gtggcgcggg cgatttttgc
     5221 cagtcgcatt ccgatagtca gcgccgtcgg gcatgagacg gatgtgacga ttgccgactt
     5281 tgttgctgat ctgcgtgcgc caacgccgtc agccgccgct gaagtggtga gccgaaatca
     5341 gcaagaattg ctacgtcagg tgcaatcgac ccatcaacgg ctggaaatgg cgatggatta
     5401 ttatctcgcc aaccgcacgc gtcgttttac gcagattcat catcgcttgc agcaacagca
     5461 tccgcagctc cggctggcac gtcagcaaac tatgctcgaa cgtctgcaaa agcggatgag
     5521 ctttgcgctg gagaatcagc ttaaacgcgc gggtcagcaa cagcagcgat taacacggca
     5581 actcgtccag caaaatccgc agtcgcgtat tcatcgcgcg caaacgcgca ttcagcaact
     5641 ggaatatcgt ttagcagaaa ccctgcgcgc acagcttagc gccacgcgtg aacgtttcgg
     5701 taatgcagta acacacctcg aagccgtaag cccactgtca acgctggcgc gcggttatag
     5761 cgtcaccagc gccgctgatg gcgcggtgtt aaaacaggtt aagcaagtga aagtgggtga
     5821 gacactgatc acacgactgg gagatggtgt ggtgattagt gaagtcagcg ccgtaacaaa
     5881 aacccgcaaa tcacgtaaaa aaacgtctaa tccgtagacc ggataagagg cgccagcatc
     5941 gcatccggca ataactccca ggctactcca gatccgccat gctggttact ggaagcaggt
     6001 caccagaccc ggtttattta ttagcgtgat actacaatcc tgtgctccgg taccttcgtc
     6061 catcccacac tgttgagagg acaaataagg aaaactgggt gaagacggcc atcccagtga
     6121 attaaaaaca gtatgcaaca actctaattc attgtatgtc ggtgtatttt ctatgctgcc
     6181 acactgcctt gcgatatccc atttactggc atcaatgata tgtgcagaag cccagttttc
     6241 gtagttaata atcggtgagg atacgggatt agccccggac ggcaattccg tcgttaacaa
     6301 cggtcgatgt agcgatttac tattcaccac taccgtgtct gccatatgtc cccaaaagtg
     6361 cgccgatgaa acatttgggc tggttggcac cgtaaaaata gcgtccagcg tgtctgtcgc
     6421 ggaagcgtaa ttagccagag taaccgtcag tggcatttta taaccagggg tattatcctg
     6481 gcggagcgaa aaacttgccg tcccatccgc gcctgtcatg ccactaatgg tattaccgct
     6541 ttcactcagc gtaacagccg aacctgataa cggctgaacg acgagatcgt ccatcgccac
     6601 tacattgcca tacaacgttg ccccagcgtt acgcggcgat gcctcaccac gtttcaaagt
     6661 aaattcaaca ttcggcaccg tattcccggc gctgtctttc accgtcacgg taacggacat
     6721 ggcttcgcct tttttcactt tcgccgcctg agtggctgca tcaaacgctg ttgaggtaag
     6781 tgtgacgctg gaaacggttg aacgcgcgtt agccaggcaa ggctgaaatg attttgttga
     6841 acccgtatac agcgtgccat ttctcaaatc aaccgactgg tctttaccat cggcggattt
     6901 atcctctgcc caccaagtgt aatacgcagg ccagccagtt tcagtgacaa acgtgttatt
     6961 cggataacgc gcagacaacg aacttaaatc attgaatagt ggctgccttg ctacttcaca
     7021 agggctcaca ctcgcatctg atttctgtgt tggggtaaag tacggccagt cttcattatt
     7081 gttattgatc ttgccgacgc cagaagtcga tgtcagttca ctccacaatt tcgggcgttc
     7141 aaataccgca ccattcactg ccgtcaaggt tgctggcata tgcccccagt agttagcctt
     7201 cgcagaatcc gggctggtca ctaccgtgaa tatcaggtcc tgattagccg taacctgcgg
     7261 atttcgcgcc aatgaagctg taaacggcgt taccagacca acggtgttat tttgggtaac
     7321 gttgaacgtt atcgtgccat cactgccggt gaagccgctc catgccgacg tattctggtt
     7381 gaaggagata cgcgccgatg tcggggtgac ttcatctaaa atgagatcat ctacggtatt
     7441 ggaggataca gagatccctg aacggctttt cgcctgcacg cgcttgagcg taacaccttc
     7501 gccgccaaca ggattaccat tggcatcttt gatcgtcacc acgatgggaa gcatctcacc
     7561 ttttttcaca gatgacgtgg gggtaccacc attgctggca gttcggccct catcataggc
     7621 agtcgatgac agcgtaaggc ttgctggtgc cgaatgtggc tgcgtcagac agagcattaa
     7681 agcgtatcca ttaatagtcg tcgtcgattg tcccgttttc aaattaatga cgccgtaata
     7741 ccacgtcaac gcgtcagtgg tgctcatttc ataagtccac caatttccac ttccggcagt
     7801 tataggcaac cccaaatctg tcagtattgt cccgcctgga tgatcattat atagcccctg
     7861 taactcattg agacgcggct gatactcaac cgggcaatcc ggcgtaactt tattggtata
     7921 aaaaaggtat gaccacgact cattattatt acttaccgag gaccccaagg tcgcttctgc
     7981 tgccagtagc ggtcgtttaa aggtcacccc cgccctactg gtaaacgtgt cgggcatatg
     8041 accccagtat tttgctttcg gtgagttcgg actggttatc acagtgaaaa taacatccat
     8101 cgtggcactt tttgtggtgt cgcgcatcaa tgttgcagtg agcggagttg ccagcccata
     8161 agtctcgttt tgcgtcacat caaaagtcgc tttaccatca cttcctgtcg tcagaagtgc
     8221 ctgtgcccca ttattataga gtgacatgtt tactccggca ggggtgaccg gcacgatagt
     8281 aagatcatca tacgtagaat ctagcctgtt ttttgcacga tccaacgcag tcccgcgtat
     8341 gagattaaag tttgcgcctg gcaacagatt tcctccgcta tcccgcacaa tcaccgtcat
     8401 ccggatcgcc gtacctttcg ccgccgttgc actagggcga ccatcgttgc tggccgtttt
     8461 ctccgcatcc agtgccgtgg aggtcatctc tatagaagct gccactgcat gagggtttac
     8521 cagacaggac acaaatgcca cggtgtttga taatgccctg gagcttgatc cattcgataa
     8581 attaatagac tgatcgtacc agacgttcgc gtaggcaatt ttatcgtaag cccaccaggt
     8641 gttagtcagc ggaaggccta aatcggtccc tatcgcacca ttaggatgtt cactgtataa
     8701 ctcttgaaat tcgcttaata acggttgata aggttcgccg cagtctgatt tgctgacatc
     8761 attttttgct gccgtattca aagaaagcca tgattcatta ttagccaggg ttgactgccc
     8821 cgctggatac tcggcagtaa gcaatggccg cttaaaggca attcccgacc gggtagtaaa
     8881 cgtttcaggc atatgccccc agtaacttgc cagtggcgta tccgggctgg ttatgacggt
     8941 aaacatgacc ggtaatttgt tggattctat cgtcagctga tacaactggg cgtacatatt
     9001 ggttaccagg cccatagaat catcttgttt taccgtaaag gaggtagtcc cgtccgcgcc
     9061 agtcacccca taaagcaggc ttgaagaact attcatcctc gcagaagcgc ctgccgcgtt
     9121 aattaccgta atcgacttat ttatcatctc ggcattttga cggttcttcg agccttcacg
     9181 tctgagaata aaggggacat tgggcacgct atttccctgc gcatttttag tcaccacgcg
     9241 aatcaccgcg tcttcgcctt ttttcacttt caacgcactc aaggtgtcgt caactttgac
     9301 aaattgcgcc gtatcattcg cctcaacaat gatctgactg gcaacggcga caggcgtggt
     9361 ccggcagctc agtaatgcac caccagatga gggcatctgc gtttcatctc ctgtcttcag
     9421 cgaaacgaca tcatacgtaa aggtttgagt gaacgtatct gcaacggtat tcgagatata
     9481 gaacctcgac actggccagc caatagcatc atgtaatgcg ctactcccct ggtgcgcata
     9541 aatggcccta agttcattgt cagttggaac atactccgcg ccacaaccat ttatcgaggt
     9601 atcgttagcc tggcccgacg ggaacgtaga ccaggcctcc ccctcttcca ccagggtacc
     9661 gacactggtg gtagcactat tctcgtccct cagcaaaggc cttcgataaa taagaccgtc
     9721 atcgcctgtc gccgtttcag ccatatgccc ccagaatttg gcttttggcg aatccgggct
     9781 ggtaagcacc gtaaagacaa cgggaagcgt cgatttagtg ctgctggagg aatccaacgc
     9841 tgccgtgagt tctgttttta gcccggtagt ctggtcttgc ttcaatgtga aagtggtgat
     9901 gccatcagca cccgtcacgc cataaataac cagggtcgtt gaaagaaaat catcttgagt
     9961 atttccccag gcatccgtta ccgccagcga agcaatcgac gtcgtagttg acacattggc
    10021 cctgttcacg ctggtgtttc gtttgagggt aaatgcggta tttcccacag ggttaccctg
    10081 cgcatcttta gtggtcactc gtaatacggc ttcttcacct tttttcgctt tgacaacatt
    10141 attcatacgg tcaaattttg tggggtctgc agcttcaaga acaatttggc ttgccttaac
    10201 aatcggcgtc gtctggcagg taagcagttc ttttgtggca gatggaatta tcgaactggt
    10261 gcctgaactg agattgacgg ccttgaagtc acgctgttcc aggctactac tacctgtcgt
    10321 gctggaaagg taacttgagg cgacgggcca gccctgaacc gttttcatcg cattgccgcg
    10381 gttggcttca tacaatgact ccagcccggc ctgggaggga acatattcac caccacaacc
    10441 attagaggat gtgcttatag cctgggtttg ggtaaattgc gcccagtctt cattctcttc
    10501 tgcaatcgct gttcgccccg acgtactgga taactctttc aacaataatg ggcgtttaaa
    10561 gaccgttcca tctgcagccg tcactgtttc aggcatatgc ccccacattt tcgccttatc
    10621 gctgtccgga ctggttacaa cggtaaaaat ggtatcgagg gtcgcttttt tcgttgtatc
    10681 tgaatagagc gtagcggtca gcgacgtttt cgtgccatgg gtatccgggc gggtaatagt
    10741 gagaatttta gttccgtcgc tgccggttat ggcgctgtat gctgcagcgg tattattaag
    10801 cgaagtttcg tccgccaggc caccattgac cacaaccggc gcaaccagcg catcaccgct
    10861 accagcaacg tgtttttcct ctgagcgggt ataaccatcg ccacgtttca gggtaaaggg
    10921 tatatcaccc aatggattgc cctgcgcatc ttttaccgtc acctttacct gtagcgtttc
    10981 gccttttttc agcttcgccg catttgcagc cgcgttccac tgcgccggat ctaccacttc
    11041 cagcgtaatg ctgctggcag gattgttagc cgtcgtcagg caactgacat aaaccgccgt
    11101 gctaccgcca acggtccgcg ccccatcgtt gagggctatg gtgtaataat gcggtacctg
    11161 atctgcgggc gaactgctcc agtagggctg acgctgcgtt ggccagccgt gtactgtctg
    11221 tacaccgtta ccgttattgg catcatagag cgcgctaagc tgagaacggc gtgggagcat
    11281 attggcgcca cagccacctt tactggtgct ggaggcgttg gcctggctga ctcgcgacca
    11341 gacttcgtta ttttccgtta atgtgccgtc cggactgaac acttccgcag ccagtcgggg
    11401 tcgggcaaag gttaaagcat cgacggtaat cgtctcatcc atatgccccc acatctgcgc
    11461 gccaaccgca tccggactgg tgagcgtagt gaaaatcaca ttgtaattaa cagtattggg
    11521 aatataggag ttcacaggca caatatttag cggcgtcgat aagcccacac cttgcgattg
    11581 cttaatttcc acagtcgcaa aaccctgcgc atccgtagta ccggcataga ctaaagacgg
    11641 ttgcgatgtt ccgtagagtg tgccattcat ctcgattgcg ccgcgggttg gatcatcaaa
    11701 acctgtagtc tgcccctgac gatttttccc catatcttta gtgatggtga acgcggtata
    11761 cggcacgggt gcattattca aggcattaaa ggtgcgtacc gtcatggtga tcgtgtcacc
    11821 cactttggcc tgcgctctgc tgcctgcgga aacatcacga tcggtggtcg cggcaatctt
    11881 ggcgaccatc tcattgcccg aacaagagag atagttttgc ttaaagccgg aataggtgtc
    11941 gatgttgccc gtggccaggt ttaccgacgc gtgcgacgtc tcttccaccg cactaagata
    12001 cgctttttgc aacgttggcc agccatattc ggtgccgatg gcattggctg gatgagcggc
    12061 aaacaaactg tgcaatgaac tttggctcgg aatatgacct aaaccacact cagcctgcat
    12121 actgctattc tgatcaaaca gcgcccagtc ttcgttattt tcccgcaccg atccctgttc
    12181 gttgtcggtt tcatccgcca gccgtgggcg tttaaagata ttgttcgctt caataatgcc
    12241 caacatatgg ccccacatcc gcgctttgtc ggtatcgggg ctggtgatca ccgtaaaaat
    12301 aacgtctttc tcatccgagg cagtaaaatc actgcgcatt ctcgcggtga tgtgagtttt
    12361 cacccccacg ccgcctggct gggtcagcgt cagagtggcc tctccgttag catcagtgat
    12421 cccttcatag gtatgggcgt caactttgcg aacgttactg ccgccatcaa tttgtaccgg
    12481 atgcgtctcc cacgcggcgt cagtttgatt cttacggtta atgccgtcat ccagatgcag
    12541 cgaaaagtag tagtaagcca gcggctgatc gttgttcttg ctatcggtaa tcgtcagacg
    12601 taaagaagcc tcttccccca cctttacttt catcgctttt atcgtcgaat catagctgcc
    12661 cggcgtcagt accagcttcg gttcaacatc gggcgcttct ttatcgacac agctcacaat
    12721 aaacgtcgta tcgtccggtt tttgcgcttc gctgcggttc gaaacatccg ccgcatggtg
    12781 ctggccgctc atccccgcca gtgatgacca gtagaaatta ccctgcatcg gccaaccagc
    12841 cacatcctgc actgacgttg gcaccaccgt cgccagcgca ccgaactgac gcattccggg
    12901 caaaatgtcg caatgtttat ccgcgccgct ccaggtaaag gtggaccagg tttcattgtg
    12961 atcgttaacc gtaccgcctt cattatcgac ttccgccgcc agttttggtc gactaaaagt
    13021 gtaatcacgg gccttcagcg tatcgggcat atgaccccac attgtagcct gcggaacatc
    13081 cgggctggtg aggacggtaa agatcactgc cgtttcactg gcctgagcaa tacccgcgag
    13141 gctcgcgacc aacggcgttt ttacaccagg gccgttggcc tgggtgaccg taaccgtggc
    13201 aacaccattc gcatcggtag taccacgata ttccgtcgcg gtggtggtta attcggtcgt
    13261 gcccagtttt acaggcgcag tattgttcac cacaccctgg cggttttcag catctttgcg
    13321 tttgataaca aaaggaatat tccccacaac attaccgacg caatctttgg tggtgaccgt
    13381 taacgtgata ctgcccccaa ccgtcatttt cgccgcacct gaggattcgt catagcccgc
    13441 agcgctgacg gaaataaggt caccctgcgc gggcgcaatc ttcagcgtca ctttctgatc
    13501 ggcgagcgtc agaacacttt catagggata gcccgtcgag gtgagtaaat ataacagcgg
    13561 catggtctgc tggggatcag gctgcgccca ggcgaccagc gaacgcccgg caagtccctg
    13621 tgcacaaaac gagcgatctg cgactggctg tgggtccagt aaggtgtccg gcgtggcggc
    13681 atccgcccaa actaaagata cagccggtgg cgtttggttt tcccaacgtg gggtggcaat
    13741 gatatcgcct tctctgtctt gcgggttggt gacgagcaat ttagagacgc taacatccgc
    13801 gttgaattca ctcggtttag cggtaaacgc tacctcatgc gtctttgtag gatccagaaa
    13861 gacactgccc tggtagacgg gtatgctggc gctgtcggcg gtgggcacgg taccgttaaa
    13921 ttcgccgacc gtatctgaag agtcttgcca tgcagattct gcccatgacc acccaggcag
    13981 cattgcgctt atcagcaatg cgcataacac aaggatttta ttcagggtca gggacatgac
    14041 agaaactcca ccggttgtta aacggacgct tgtcgcatca acatattttg cttaatccgg
    14101 gattaagcaa aatatgcgga aacagcattc ccttgcggga atgctgctga gtacgcgtta
    14161 tttgtgtttg taaataatgt gaagtttgta accctgtacc ccgtcgccgt tggtcgggat
    14221 ggaaagtgcc tcttttccat cgcgcgcagg gccgttaagg ttggttctgg cttcgtcgtt
    14281 ggtcgcaggg atgaccaaat tgttattatc gaagcttgag ttaacgatgc cacccgaagt
    14341 gccaagctgt tggctgctgc cagaaagtac ccaacggaag tcataattgg cggtgacgtc
    14401 agcatcggta cccgcatcaa acttgccatt tttgttctca tccgagtaca gtttggccac
    14461 gtaggttttg ccaagttcga ttggcgtgct accgttgata aggttgatac tggtaccatc
    14521 aacaaagatg gcgactttca ggttctggtt aaccaccggc cccggtgcaa cgttatcgct
    14581 gtcgcttccg ccgccgctgg cagtagaaac gtcataaaga tgcagcgcag tcccgacgtt
    14641 tggcgtaccg gtctgcgtgg ttggcgtaat ttcaataccg atataacgac cacggtcggc
    14701 atcggtaaga gtgtaactgg tcaccttcgt taccagctct tttggatcac ccccctgcgc
    14761 atccttgtag ctcatccagc ggatagtatc gatagttttc gcgttatcgc ccaatccccc
    14821 aagatccacg tcgccttcgg tatcaccaat ttcccaactt acggttaatt tatcacccga
    14881 gtaaagacgc ttgtccgttt cactgccttg cgggcgagcg ccttttagat cagaagtcaa
    14941 cgttacacgg tcggcatctg tcgtagcact tcccccttcc gaatagagcc acggaatggt
    15001 cccctggaaa atattggttt caccgctgtc gacccaggcg ctgtcttcag ccagcgccac
    15061 aggtgccaca caataacctg ccagtgccag cgctaatgcg atccgggtca gaccgcgtct
    15121 tttatcctgc ttcattgatg ctctccttaa tgaaccttca gcgactggcc gactggcctt
    15181 acgccaatct tattttttgg tatacagcac gcgtaaaccg tacccctgca agccatctcc
    15241 cgcttgtgcg ccataggttt gcgccgcttc acggttagtg gccggaatca caatgtcctc
    15301 attggaagtg ttcgcctgtg cacccacttc accattgatg ctcttgccgg taaattccca
    15361 cttgtagtcg tactgcacca tctcttcgtc agtcagtttt tcgacctgct ggaagacacc
    15421 gtctttattg ttgtcgcgcc ataaaacaaa tcgataaatg ttgttgagcg gcaacggttc
    15481 tttatcttta cctatcaggt tgctgggttt agccgccccc acacgataga taaaagcatt
    15541 tagcccacca atttctgccc cgaggaaagt gacatccaca tagttactgt catcgtaggg
    15601 cgcggctttt gctttccagc gataggtgcc gggtaaggta ctggagaggg tgacagtgcc
    15661 cttttcttta tcttgtttgg caataaaaac gcaggggtta tcggtcgagc agggcttgtt
    15721 ggttaacgtc actcgtttgt cctgcgcgtc gtaaagctga ggcgtaaggt tatcttccgg
    15781 caacgcatcg ccgttgcggt cggtgttatc gccaaagctg tcggttacat cgagatccat
    15841 cactacacca tctacaccat cggcctgtgc gctggcttcg tggctgtggt tgttctcttc
    15901 tggcacgttc gcgatgttat taacaatcaa ctctatttta cggtcctgct gaacggtgat
    15961 ttccaccacg ccagagttct gtcggttgcc cttttcatct tccagcgcca gacgtactcg
    16021 ccaggtattg agtttttctt gttcggcttt gtcagagctg tactgccagg cgggcagcac
    16081 caggttccag tggttattgt taccctctgt cagggtattc tctttgataa ccggcatcac
    16141 cagcgtgcgg gcagcattgt ttttcgcctg ccaggaggca gcaacgattt tgtagtgatg
    16201 gttaatgagc gcgttaatgg tccatttaca tttttccagc ccgatagctt cttcaggcgt
    16261 atctttaatc acgcactcgg gatgttcatt cgtggtgtct gctttcagcc acaaaatgac
    16321 gtcgagggga tctttttctt tgtattccag aacaataaag ttgttacgtt caacaaagtc
    16381 atgacggcta cccatgagcg agtgagccgc gctcactttg tcaggatcaa gctggtcttt
    16441 gagcgggacg ccaaactggt agttcagcgt tgcgttaaca gaaagatcgg tattatcgcc
    16501 agtgccagct ttgaaatctg taccgacaac aatgagcgga acgggctggt atttcacacc
    16561 cagcgtgaca gcgaagggat ctttctccag actatcggta ccaaaaagcg cgacttcatt
    16621 cccataatat tgttcgaaga caatttttcc gccaagctgc gggtaagcgg gtagccaggc
    16681 ttcagcgcga atatcccagc cgcgcgcagg gcgttcttca taaaagtcga aatcttcaga
    16741 gtctttccag tcagaaagtg ggtgataata gttgcctgag aattttaaat aatccgtcca
    16801 ggcttcggcg cctaaaccta aacggcgatg tccacgggta aagtcataat cataaaatat
    16861 atttccaccc aatagatatt tatcaaaatt ataccttacc ccaaggccaa tattcccgat
    16921 cgtacgatct tcttttcgtt gcgcagaaaa ttggctgaaa taaacagtgg tttgattatc
    16981 gtaccaggga acaaaataat cgatggaact gccatccaga tcgccgccct gaccaatgga
    17041 gaggttcgaa cggaccttac cgtagggaga gagtgtgtct tcaatataag attgtgcggt
    17101 tgaggtgatt tgactttgca gatagcttct ggcctgagat tccagcgcct caggggtcaa
    17161 gttttcaaac ccttgcgtgg cggagttgat gacgtaatcg gctccgcgct ctttcagcga
    17221 tttacccttg ttggtttgtt catcctgttg tgctgcctgg ctacctaaat caggtaacga
    17281 ttcgttttgc ttaacattta tctctggcgc cgcgaaagca gcaccagaga taaatgttag
    17341 aataatacag cgtttccatc gcagcatgca ctttccctgt gacaatgata cagtcaaaaa
    17401 taagctttaa tattgtatc
//
LOCUS       DADTFG010000105        17203 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.106, whole genome shotgun sequence.
ACCESSION   DADTFG010000105 DADTFG010000000
VERSION     DADTFG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.106"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..218)
                     /locus_tag="J1138_003218"
     CDS             complement(<1..218)
                     /locus_tag="J1138_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBA8454981.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEP"
     gene            357..731
                     /gene="yobA"
                     /locus_tag="J1138_003219"
     CDS             357..731
                     /gene="yobA"
                     /locus_tag="J1138_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBA8454982.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            735..1607
                     /gene="copD"
                     /locus_tag="J1138_003220"
     CDS             735..1607
                     /gene="copD"
                     /locus_tag="J1138_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBA8454983.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGAGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            1620..1961
                     /locus_tag="J1138_003221"
     CDS             1620..1961
                     /locus_tag="J1138_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBA8454984.1"
                     /translation="MMRKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            2356..3012
                     /gene="pphA"
                     /locus_tag="J1138_003222"
     CDS             2356..3012
                     /gene="pphA"
                     /locus_tag="J1138_003222"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBA8454985.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRSVRGNHEQMAMDALASQQMSLWLINGGD
                     WFIALADNQQKQAKTALEKCQHLPFILELHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(3013..3288)
                     /locus_tag="J1138_003223"
     CDS             complement(3013..3288)
                     /locus_tag="J1138_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBA8454986.1"
                     /translation="MAGYLSGLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDIYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(3309..3545)
                     /locus_tag="J1138_003224"
     CDS             complement(3309..3545)
                     /locus_tag="J1138_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBA8454987.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(3663..5102)
                     /gene="rsmF"
                     /locus_tag="J1138_003225"
     CDS             complement(3663..5102)
                     /gene="rsmF"
                     /locus_tag="J1138_003225"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBA8454988.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVTDFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(5182..7815)
                     /gene="yebT"
                     /locus_tag="J1138_003226"
     CDS             complement(5182..7815)
                     /gene="yebT"
                     /locus_tag="J1138_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBA8454989.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPDGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(7784..9067)
                     /gene="yebS"
                     /locus_tag="J1138_003227"
     CDS             complement(7784..9067)
                     /gene="yebS"
                     /locus_tag="J1138_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBA8454990.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNACFDD"
     gene            9197..9694
                     /locus_tag="J1138_003228"
     CDS             9197..9694
                     /locus_tag="J1138_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBA8454991.1"
                     /translation="MNKAEFYADLNRDFNALMVGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDERGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            9791..10489
                     /gene="proQ"
                     /locus_tag="J1138_003229"
     CDS             9791..10489
                     /gene="proQ"
                     /locus_tag="J1138_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBA8454992.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            10509..12557
                     /gene="prc"
                     /locus_tag="J1138_003230"
     CDS             10509..12557
                     /gene="prc"
                     /locus_tag="J1138_003230"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBA8454993.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIAHFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            12749..13630
                     /gene="htpX"
                     /locus_tag="J1138_003231"
     CDS             12749..13630
                     /gene="htpX"
                     /locus_tag="J1138_003231"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBA8454994.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(13676..15049)
                     /locus_tag="J1138_003232"
     CDS             complement(13676..15049)
                     /locus_tag="J1138_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8454995.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            15226..16017
                     /gene="kdgR"
                     /locus_tag="J1138_003233"
     CDS             15226..16017
                     /gene="kdgR"
                     /locus_tag="J1138_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBA8454996.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(16161..16400)
                     /locus_tag="J1138_003234"
     CDS             complement(16161..16400)
                     /locus_tag="J1138_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454997.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            16559..16702
                     /gene="mgrB"
                     /locus_tag="J1138_003235"
     CDS             16559..16702
                     /gene="mgrB"
                     /locus_tag="J1138_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBA8454998.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            16777..17064
                     /locus_tag="J1138_003236"
     CDS             16777..17064
                     /locus_tag="J1138_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8454999.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         4471 a         4182 c         4397 g         4153 t
ORIGIN      
        1 ggctcgtaag gtaaacgtga cagattgacc gaagccaaac ggtgggcgat aagccgctcg
       61 cgaaaccagc tgcgcaaatg ttcaggctgt tcacgttcaa ccgcttcagc gatcactggc
      121 atattgtagc gttctttaaa tgccacccct gccgccgcca aatcgacatt cactttatcc
      181 atttctgttt gatccagttt agccagattc ttcagcattc ttaatctcct catcattcgc
      241 gggcaaaagt tactaagagt gagccttgat ggcaagccgg aattgatgat cctcgactca
      301 gacattagac aaggttatgc tctgtaaaaa agttattaca aaaaggaata gataatatgg
      361 cttcaactgc acgctccctt cgctacgcgc tggcaatcct gaccacttcg ttggtgacac
      421 cttccgtctg ggcacatgcg catttaacgc atcagtatcc tgcggcaaat gcacaagtga
      481 cagcagctcc gcaggcaatc actttaaact tctcggaagg cgttgaaaca ggattcagtg
      541 gtgcaaaaat cacggggcca aaaaacgaaa atattaaaac attaccggcg aagcggaatg
      601 agcaggacca aaagcaactg attgttccac tggccgattc gctgaaaccc ggaacctaca
      661 ccgttgactg gcatgttgtt tcggtggatg ggcataaaac gaaaggacat tacaccttta
      721 gtgtgaaata aatcatgctg gcgtttacct ggatcgcgct gcgatttatc catttcacct
      781 cgctgatgct ggtttttggc ttcgcaatgt acggtgcctg gctggccccc ttaacgattc
      841 gtcgcttgct gacgaagcgt tttctgcgtt tacaacagca cgctgccgtc tggagtttga
      901 tcagtgccac tgcaatgctc gccgttcagg gcgggttgat gggagcggga tggtcagatg
      961 tattttcgcc aaacatttgg ttggctgttt tgcaaacgca gtttggtggc gtctggttat
     1021 ggcaaattgt tctcgccctc gtaacgttga tcgtcgccct tatgcaaccg cggaatatgc
     1081 cgcgtttgct gtttatgctc actaccgcgc aatttattct gctggcggga gtcgggcatg
     1141 cgacgttgaa tgaaggggta acggcgaaaa tccatcagac taatcacgca attcacctga
     1201 tctgtgcggc ggcgtggttt gggggattac tgccggtgct ctggtgtatg caactcatca
     1261 aaggccgttg gcgacaccag gctattcagg cgctgatgcg tttttcctgg tgcgggcatt
     1321 ttgcggtgat cggcgtactg gcaagcggcg tgcttaatgc attgttgatt accggatttc
     1381 cccccacgct tacgacttac tggggccaac tcttattgct caaagccatc ctggtgatga
     1441 ttatggtggt catcgcgctg gctaatcgtt atgttcttgt accgcgcatg aggcaggatg
     1501 aagatcgtgc ggctccctgg tttgtctgga tgactaaact cgaatgggcg ataggtgcgg
     1561 tagtgctggt gatcatcagc ctgcttgcga ctctcgaacc tttttgatgg attacgaaaa
     1621 tgatgagaaa aagtattctg gcgtttctgt tactcaccag ttctgcagct gcgctggcgg
     1681 caccgcaggt gattaccgtc agccgttttg aagtgggtaa agacaaatgg gcgtttaatc
     1741 gcgaagaggt gatgctgact tgccgaccgg gtaatgcttt gtatgtcatt aacccaagta
     1801 ccctcgtgca gtatccttta aacgatatcg cacaaaagga agttgccagt gggaagacta
     1861 aagcccaacc catttcggtg attcagattg atgatcctaa caatcccggc gaaaaaatga
     1921 gtctggcacc gtttatagag cgagctgaaa aactctgtta attacctaaa atagcctttt
     1981 gatttccaat aaaaaaccgc ctcagttctt tcaccagaac gggcggtttt taacatttaa
     2041 gctgatgacc accacgcttt ttattgacca ttttgcacgc aaactggaaa acctggcgtc
     2101 gtcatctatt cttaaagagc aaggcaacta agcctgcatt aatgccaact tttagcgcac
     2161 ggctctctcc caagagccat ttccctggac cgaatacagg aatcgtgttc ggtctctttt
     2221 tatctgttaa aagccagaag cattcccttc gctgacttta tagtcaacca taacacacac
     2281 tctactgtct gagtccagcg ttttttaaca ttcttgttaa gattatgtga tctttagcgc
     2341 gggaggaaaa tattgatgaa acagcctgcg cccgtttatc agagaattgc gggtcatcaa
     2401 tggcgacata tctggctttc aggcgatata cacggttgtc ttgagcagtt gcgccgcaaa
     2461 ttatggcatt gtcgttttga tccgtggcga gatttactta tctcagtggg agacgttatc
     2521 gatcgtgggc cgcaaagttt acgttgcctg cagttactgg aacaacattg ggttcgttcg
     2581 gtaagaggca atcatgaaca gatggcgatg gatgcgctgg catcccagca gatgtcttta
     2641 tggttgataa atggcggcga ctggtttatt gcgctggcag ataatcaaca gaaacaagcg
     2701 aaaacggcgc tggaaaaatg tcagcatttg ccctttattc ttgaattaca cagtcgcacc
     2761 ggcaaacatg ttattgctca tgccgattat ccagatgatg tctatgaatg gcaaaaggac
     2821 gtcgatttgc atcaggtctt gtggagccgc tcgcgattag gtgaacgcca aaaagggcag
     2881 ggaattgcag gtgctgatca tttctggttt ggtcatacac ctttgcgcca tcgcgtggat
     2941 attggcaacc tgcattatat tgataccggt gctgtctttg ggggagaact gactcttgtg
     3001 caattgcaat aattaaaaat caccgtactc ctgtgcaggt cgccagaaac catctataaa
     3061 atcctcaatc ggaaaacaac cgccatggcg gatccgttga tcgctcatag aataaagaca
     3121 ctgctgttcc gtgttgtaga tatccacaac aatatcttca caaccgccat ccaggtagca
     3181 aacaaaaagt accagcgcga acatttcatc cccgaagtgt ggtgccgtac cgttaagttt
     3241 aggagagatt ttacaacggg ggaataaccc ggacaaataa cccgccatgc cgacgggttc
     3301 tttttggatc aggcaagacg cataatccag gcatcagatt gttgatcgta acgggtacgg
     3361 taaaggacag agtgttcgcc atttagcagg gcagggatac tggtttcagc atcccaggca
     3421 tccagttgca tacataaatc ggggtctgat ttacaaggaa tggttaacgt tcgatcaccc
     3481 ggcgattcgc cgtgtaattc gccgtcgtcg atttcaaaag cgcctatgcg cacactggtt
     3541 ttcatcttcg tgctctccat ttagctgccg gttgtggtac gaccagtccg atcgcaccat
     3601 ttttcagcct agttgaggca gttgaatgtg ccagtcaaaa agtgcgcttt ttttgtgcgc
     3661 tgtcaggcgt taccggtaaa aagtttcccg tcgcgcacca gttcacgcgg gtagctgttt
     3721 ttcagtcgtg aaccaatccg tttggctaaa ccaatcggct gatgctggaa ggtaaccaat
     3781 acatcatccg ccactggcgc ggcttgcggg taaacatcgc gcccgcgata ccactcttcc
     3841 gcttcctgcg gtgtcagttc aaaagcgttc acattgtcgg gggaggcaag ggcaataacg
     3901 gcttcatgct gccagcgata acctttgttg tgcgtttcgg caagtttaat ccccaggcga
     3961 gaaaatcgga ctttaccgat cagggcttca atgcctaccg ggaacaacca cagttctttg
     4021 tcacgctgcc agagtcgcag gttttcatcc cagtttaagc caacacttgc agccgcctga
     4081 cgaatttgac cggcttcgcg atctttcacc gggctgaacg ggaaattgcc cactttgtat
     4141 tttggggtgg gtaaggcggg aatcgcctga gttttacgca gacgagcaac gaagaagcct
     4201 tcgcagtcgt aaatttgtgg gaaaacatgc aaaaagcctt cctcggtcag cgccttgttt
     4261 gcaccaggga agagatcgcc aagcggtaaa aactctactg cgtcgggata agtctctttc
     4321 agccacaggc aaacggcttc gttttcttcc cggtttaagg tacaggtcga gtaaaccagc
     4381 gtaccgccag gacgtaatgc atgaaaggcg ctgtcgatca gttcccgttg ggtcgctgcg
     4441 atttcctgat tgctttctgg tgaccagttt tttagcgcat cgggatcttt acgcaccacg
     4501 ccttcgccgg agcagggagc gtccagcaaa atggcatcga acatttctgg cactgccgca
     4561 ccaaatacgc ggccatcaaa atgcgtgagc gcaacattac tgatgccaca gcggctgata
     4621 ttggcatgta acacttttac ccgactggcg gaaaactcat tggcaaggat tgccccttcg
     4681 ttattcatcc gcgcagcaat ttgcgtcgtt ttggaacctg gtgcagcagc gacatccatc
     4741 acccgctgtg gtgcattacc gtcagcaaac aaggcggcaa caggcaacat tgaactggct
     4801 tcctgaatat aaaacaggcc gcttaaatgc tcggcggtac tacccaacgg caatgcatct
     4861 tcattgtcgc gttcaatcca gaaaccttct tcacaccacg gaattggcgt aagcgtccag
     4921 ccataaggag cagttaattg caggaaatca gtaacggagg ttttcagcgt attaacgcga
     4981 atgctgcggc gcaacgggcg ctgacaggcg gcaagaaaat catcaaaaga gagcgtcgaa
     5041 ggcatcgctt cgcgcatttg tgtcagaaag gcgtccggga aataaacggt gtgttgggcc
     5101 acgagcatat accactggca aaaaaacagg cgcgcagttt agcataaacg ctccggcgca
     5161 ggcacgccgg agcagtgggc attatttggg aagcgcagtt ccccattcac gccactcttt
     5221 cggttcactt tcctgcaaca ggaagtgctt gccttcctgg gctttcggtg ccagtggcgt
     5281 acccggaggc gtggcgaagg cgatgccgcc acggataaac tggttaaagg tgccggtttt
     5341 cactacgccg cccgtcagac caaagtccag actgtaaccc gatgccaacc agaagacgga
     5401 attgttacgc accaggtgtt gatagcgttt actgatgcgc atcgcaatca tcacgcgatc
     5461 tgacaatgtc cccagcgtca ttccggtaac cgtacctact tccagaccac ggaacagcac
     5521 aggtgtaccg atacctaacg aaccggcttc cggcgcttca acaataatgc ttaagccatc
     5581 caggtaacgc gaatcagtaa tggtggcctc ttgtaattca aagtcgcggc gaggattgcc
     5641 ccggcctggt tcgacgttga tatacggctg gaggatagta tcaagatgct caacgccagc
     5701 tgccgaaatt tgcggtgtga ccactgagaa gcgcgtacca ccgcgagcaa aggtctggac
     5761 atattccgga tagagcaccg cctttgcctg tacttcattg cgtgcggtaa tcagatccag
     5821 cgtctggatt tgcccgatat caataccgag atagcgaatt ggcatcccga ccgccagttt
     5881 tccggcatcg aaagcgtgaa gcgtaatttg cccgccaacc gcacgggccg ctgtttcgga
     5941 agcatacaga atacgcttgt cgcctttacg ctgactggcg ctggcaccgc tgaggttatc
     6001 gaagctaata gcccccttta atgctctgga gagcggggat gcctgtacag tcagaccact
     6061 accattcagc tgaactttcg ccccaccttc tgcccagaac acgctgttgc tggtcagaag
     6121 gttgcgatac tccggcttaa tatgcagatc gatatcaaac gcgttagctc gcggacgcac
     6181 agtaataact tcaccaactt caaatttacg gtacagcact accgatcctg cctgcacatc
     6241 cggcagcgtc tctgcactca aactcacggt tgtggtgggt aaatcgctaa ggctgttctc
     6301 cagcgctttt tccagattgg catacagtgg atagctggct ttcatctcgc ctttatcgcc
     6361 cggcagaata cgtattccgc cattgatcca ttctgaggcg ctggcaccga gaaactcaac
     6421 gccatccagc cccaccttca cgtcgacacg gctgttgacg acaaatttgc tatcgccttt
     6481 taccagttcg cgatgctgag gctcgatggc gacggtaaag gtgacgcctt tgctggtgag
     6541 tttacgatcg ataacctggc ctacctgcac gccgtgaaga atgagcggct gaccagcatc
     6601 aataccgtaa ctttctggtg ctgtcagggt cagtgtcaga acatccggct cttgcagcag
     6661 ggctttttcg cctggcacaa caacaaactc tttgcgtggc tcgccatcgc cgggcaccaa
     6721 ctcaaaggtt tttccggtca gcagggcgct gagattggca tcgctaaggg ataatttcgg
     6781 gttgcgtaat tcgatgcggg tattttcacg aagcagggtg acgacgctgg gatcaacggt
     6841 catttcccca gtgactttac caccaggatt taaatccagt ttagtcagct gtccgacttc
     6901 cagcccctga tacattaacg gcgtcgagtc ggcggttaat ccggcaccgt ctggcagttc
     6961 cagttttatt attacgccac gttggctatg ggccaggtct tcatacagac caaaggtatc
     7021 ttccgcttcg gcaggtttcg actcttcagg tgaatcgaag gcaatcgcac cgttaaccag
     7081 tgccgccaga ctttccagtt tcaccttcgc gccactgata ctgacgttgg catcaacgcc
     7141 ggaaacgttc cagaaacggc tacctttttt caccagatcg gtaaaacgcc gctcgatcag
     7201 gacatcaatc accacgcctt gcttgttggg attgatggca tagtcgtaga ctttacccac
     7261 cgggatcttg cggaaataga ccaatgaacc actgctcagt gaaccgagat cgggggcttg
     7321 caggtggatc atcagatcgc cattgtccag ccgatatttc ggttgggtgt cgagtgcgac
     7381 aaagtgatcc tgctctttac ctttacccgg catcatgccg atataattac caccgacgag
     7441 ggcgtccagc ccggagacac ctgccaacga ggcctttggc gtcaccagcc agaactgtgt
     7501 ctcttcgcgc agcgcatctt tcatatcgga cttgatgctg accttgactt caatcttacg
     7561 aagatcgtcg ctgaggctga tatcctgcac tgttccgact tcaacgccct gataacgaac
     7621 aggcgtacgg cctggaacaa taccatccgc cgacataaag tcgatggtga cagtattgcc
     7681 ccggtcctga taactgtccc aaatcagcca accagcaatc attagcgcga tgaacggcag
     7741 cagccagaaa ggtgagatac ggcgtttatt tttaatctgc gcttcagtcg tcgaagcagg
     7801 cgtttcctga ctcatgtgca tcccaaagta agcggctgtc cagccattcc acagcaagaa
     7861 tagtcaaaat taccgctgcg ccgaaataaa acgcagccgg tcccatagta aaagcgagga
     7921 tctgatcgcg attaatcagc gacatggtta aagatatgac aaacaggtcg agcatcgacc
     7981 agcgaccaat ccaggtcacc atccgcagta acagaatgcg tgtgcgtaaa ccttgctggc
     8041 atttaaaatg aatactcaac agtaaagtga acataacaat cactttagta aacggtacta
     8101 gaatactggc gataaacacg attcccgcaa ctgcaatgtt gctacttgcc agcgacataa
     8161 ttccggaaag aatcgtatct tcctgccgac caccattcag ataaatgata gagataggca
     8221 acaggttggc aggtaacaac aaaacgattg acgctaacag cgccgcccag catttttgca
     8281 gactatgacg gcgacgcagg cgtagcggga tatggcaacg cgggcagcga ccacgctgat
     8341 caggatagcc ggtaaaatgg cacccaagac agacacggag tttctcgtcc ctacgcgtag
     8401 cggggcgctg cggataaaat cgctcccaca gttcttcgac attaagatgt gacaatgtca
     8461 ccgtcgttaa aatcaccaac gccacaaaag agaacaagcc gacccccgcc tggatatggg
     8521 cataatcctg tacctttata gaagcaacgc caatgccgac caggtagata tccaacatca
     8581 cccactcttt cagccgctca agcatcagca gtaccgggcg taaattcatt cccaatcggt
     8641 taccaaacca taaataagct atggaggtca ccagaatgag gggggcaccg ataacgcaga
     8701 aaaagaccat cgaccccgtt atcgcatcgc cctgtttggt catttgccag atgccttgca
     8761 taacgttggc gtcaatacga atgcctaaca gccagatatg caagagcggt tcgccccagg
     8821 caaacggcat aagcaacagc atggtgaagg ccattgccgc caggcgcgtt agcgaccagt
     8881 cgcgcccgtc acgaattttt gcctgacagc gcggacaata ggcactttga tgagaattta
     8941 tctcgggcag gctaaacagc atgtcacatt gcgggcaacg ttggtaatca ccacgcggca
     9001 gttcctcgcc gatagccctc actgttatct tctttgtcgg cgtaatttgt ggtgtgttaa
     9061 gagccatgac gtgccaaatg taataagaat taacattaat cttaacccat gatgggaatg
     9121 atcttgagca ttaacgcatt taatgcttat tttaataggc tggcagttcc aggtaagaca
     9181 accaagtgat tatataatga acaaagcaga attttacgcg gatttaaatc gcgactttaa
     9241 cgcgctgatg gtgggagaaa ccagttttct ggcaacgctt gcgaacacca gtgcgttgtt
     9301 atatgagcgt ctcactgacg taaactgggc aggtttttat ttgcttgagg acgatacact
     9361 ggtgctcgga ccatttcagg gcaaaattgc ctgtgtccgg atacccgttg ggcgcggcgt
     9421 atgcggcact gcggttgccc gcaatcaagt gcagcgtatc gaggatgttc acgcgtttga
     9481 cgggcatatt gcctgtgatg cggcgagtaa ttctgaaatt gttctgccac tggtggtgaa
     9541 aaatcagatt attggtgttc tcgacatcga tagtaccgtc ttcggtcgct ttacagacga
     9601 ggatgagcga ggcttacgtc agcttgtggc acagcttgaa aaagtgcttg caacgacgga
     9661 ttacaaaaaa ttctttgcga gcgtcgcagg ataatcaacg gataacgtag catttactga
     9721 tagcgtcatt ataatgacgc ctgttcatgc ctgcgcttgt tggctacgtc cgttgtaatc
     9781 aggaaatttc atggaaaatc aacctaagtt gaatagcagt aaagaagtaa tcgcgtttct
     9841 ggccgaacgt tttccccact gtttcagtgc ggaaggtgaa gcgcgtccgc tgaaaatcgg
     9901 tatttttcag gatttggtcg atcgtgttgc cggggaaatg aacctgagca aaacgcaatt
     9961 gcgatccgct ttacgtctct acacttcgag ctggcgttat ctttacggtg ttaaacccgg
    10021 cgcaacacgt gtcgatcttg acggcaaccc atgcggtgag ctggacgagc aacatgtcga
    10081 gcatgctcgc aagcagcttg aagaagcgaa agcgcgtgtt caggcacagc gtgctgaaca
    10141 gcaagcgaaa aaacgcgaag ctgccgcagc tgctggtgag aaagaagacg caccgcgccg
    10201 cgaacgcaag ccacgtccga ccacgccacg ccgcaaagaa ggcgctgaac gtaaacctcg
    10261 ttcgcaaaag ccggtagaga aagcgccaaa aacagtaaaa gcacctcgcg aagaacagca
    10321 caccccggtt tctgacattt cagctctgac tgtcggacaa gccctgaagg tgaaagcggg
    10381 tcaaaacgcg atggatgcca ccgtattaga aatcaccaaa gacggcgtcc gcgtccagct
    10441 gaattcgggt atgtctttga ttgtgcgcgc agaacacctg gtgttctgaa acggaggccg
    10501 ggccaggcat gaacatgttt tttaggctta ccgcgttagc tggcctgctt gcaatagcag
    10561 gccagacctt cgctgtagaa gatatcacgc gtgctgatca aattccggta ttaaaggaag
    10621 agacgcagca tgcgacggtg agtgagcgcg taacgtcgcg cttcacccgt tctcattatc
    10681 gccagttcga cctcgatcag gcattttcgg ccaaaatctt tgaccgctac ctgaatctgc
    10741 tcgattacag ccacaatgtg ctgctggcaa gcgatgttga acagttcgcg aaaaagaaaa
    10801 ctgagttagg cgatgaactg cgttcaggta aactcgacgt tttctacgat ctctacaatc
    10861 tggcgcaaaa gcgccgtttt gaacgttacc agtacgcttt gtcggtactg gaaaagccga
    10921 tggatttcac cggcaacgac acttataacc ttgaccgcag caaagcgccc tggccgaaaa
    10981 acgaggctga gttgaacgcg ctgtgggaca gtaaagtcaa attcgacgag ttaagcctga
    11041 agctgacagg aaaaacggat aaggaaattc gtgaaaccct gactcgccgc tacaaatttg
    11101 ccattcgtcg tctggcgcaa accaacagcg aagatgtttt ctcgctggca atgacggcgt
    11161 ttgcgcgtga aatcgacccg cataccaact atctttcccc gcgtaatacc gaacagttca
    11221 acactgaaat gagtttgtcg ctggaaggta ttggcgcagt gctgcaaatg gatgatgact
    11281 acaccgttat caattcgatg gtggcaggag gtccggcagc gaagagcaaa gctatcagcg
    11341 ttggtgacaa aattgtcggt gttggtcaaa caggcaagcc gatggttgac gtgattggct
    11401 ggcgtcttga tgatgtggtg gccttaatta aagggcctaa gggcagtaag gttcgtctgg
    11461 aaattttacc tgctggtaaa gggaccaaga cccgtacggt aacgttgacc cgtgaacgta
    11521 ttcgtctcga agaccgcgcg gttaaaatgt cggtgaagac cgtcggtaaa gagaaagtcg
    11581 gcgtgctgga tattccgggc ttctatgtgg gtttgacaga cgatgtcaaa gtgcaactgc
    11641 agaaactgga aaaacagaat gtcagcagtg tgattatcga cctgcgtagc aatggtggtg
    11701 gggcgttgac tgaagccgta tcgctctccg gtctgttcat tccatccggc ccgattgttc
    11761 aggtccgcga taacaacggc aaggttcgtg aagacagcga caccgacgga caggtcttct
    11821 ataaaggccc gctggtggtg ctggttgacc gcttcagtgc ttcggcttca gaaatctttg
    11881 ccgcggcaat gcaggattac ggtcgtgccc tggttgtggg tgaaccgaca tttggtaaag
    11941 gcaccgttca gcagtaccgt tcattgaacc gtatttacga tcagatgtta cgtcctgaat
    12001 ggccagcgct gggttctgtg cagtacacga tccagaaatt ctatcgcgtt aacggcggca
    12061 gtacgcaacg taaaggcgta acgccagaca tcatcatgcc gacgggtaat gaagaaacgg
    12121 aaacgggtga gaaattcgaa gataacgcgc tgccgtggga tagcattgat gccgcgactt
    12181 atgtgaaatc aggagattta acggcctttg aaccggagct gctgaaggaa cataatgcgc
    12241 gtatcgcgaa agatcctgag ttccagaaca tcatgaagga tatcgcgcac ttcaacgcta
    12301 tgaaggacaa gcgcaatatc gtttctctga attacgctgt gcgtgagaaa gagaataatg
    12361 aagatgatgc gacgcgtctg gcgcgtttga acgaacgctt taaacgcgaa ggtaaaccgg
    12421 agttgaagaa actggatgat ctaccgaaag attaccagga gccagatcct tatctggatg
    12481 agacggtgaa tatcgcactc gatctggcga agcttgaaaa agccagaccc gcggaacaac
    12541 ccgctcccgt caagtaatat caatcaggca caagaaattg tgcctgattt tttaacagcg
    12601 gcaagatgcc gtaaatcaga tgctacaaaa tgtaaagttg tgtctttctg gtgacttacg
    12661 cactatccag acttgaaaat agtcgcgtaa cccatacgat gtgggtatcg catattgcgt
    12721 tttgttaaac tgaggtaaaa agaaaattat gatgcgaatc gcgctcttcc tgctaacgaa
    12781 cctggccgta atggtcgttt tcgggctggt actgagcctg acagggatac agtcgagcag
    12841 cgttcagggg ctgatgatca tggccttgct gttcggtttt ggtggttcct tcgtttcgct
    12901 tctgatgtcc aaatggatgg cattacgatc tgttggcggg gaagtgatcg agcaaccgcg
    12961 taacgaaagg gaacgttggc tggtcaatac tgtagcaacc caggctcgtc aggcggggat
    13021 cgctatgccg caagtggcta tctaccatgc gccggacatc aatgcttttg caaccggtgc
    13081 gcgccgtgat gcctctctgg ttgctgtcag caccggtttg ctgcagaaca tgagcccgga
    13141 tgaagccgag gcggtaattg ctcacgaaat cagccacatc gccaatggtg atatggtcac
    13201 catgacgctg attcagggcg tggtgaacac cttcgttatc tttatttccc gtattctggc
    13261 gcagcttgcc gcgggtttta tgggcggaaa ccgtgatgaa ggtgaagaga gcaacggcaa
    13321 cccgctgatc tactttgcgg ttgcaacggt tctggaactg gtgtttggta ttctggcgag
    13381 cattatcacc atgtggttct cgcgtcatcg tgaattccac gccgatgcgg gttcagcaaa
    13441 actggttggt cgcgagaaaa tgattgctgc attgcagcgc ctgaaaacca gctatgaacc
    13501 gcaagaagca accagcatga tggctttctg cattaacggg aagtcgaaat cgctcagtga
    13561 gttgttcatg actcacccgc cgctggataa acgtattgaa gctctgcgta cgggtgaata
    13621 cctgaagtaa tatgaagtaa atactaaaaa gcgcgtcctg atcgacgcgc tttttttatg
    13681 ccctggatcg tggctgagtg atacgtaaac cactgacaca ggcagcaatc actgccagaa
    13741 tagccgcagc tatcagcgag acgtgcgtac cattatcacc aaactgattt agcatcagcg
    13801 ctaccagagc cgcgccgcta ctctgaccca gaagacgagc tgtccctaac atgccactgg
    13861 ctccaccgct acgttcgcga ggagcggagg taataatggt gtggttattg ggtgactgga
    13921 acaagccaaa tccagcgcca cataagatca tcggccagat aatattgata tccgcaggtg
    13981 acgcgggcag cagaaccagg gaaaaaagcc ccgcagccat gatgaataac cctaaagcac
    14041 ccagcaatcc tgcatgtacg cgttcaatca aatagcctgc cagtggagcc atcaccatcg
    14101 ttgctaacgg ccacggtgtc agaagtaaac ctgtttcgac ttcactacgc ccgagcacgg
    14161 tttgcaggta aaagggaagg gaaaccattg ccagcatttg tgcgcagaaa gagcaaacag
    14221 atgtgcaaat agaaagtgaa aacagcggga tacgcagtaa atccaccggt agcagcggta
    14281 cgggaagaga aagctggcgg cgaataaaga aaataccaac gacaaccatt accaccagtt
    14341 ccgcaccaat caatgtcagt gattgcccct gagcgaaacc actcaatgca gtaataagca
    14401 ggccgaaggt taacgcgttc atcacggcgc tgggcaggtc gaaacggggt ttactggcgc
    14461 gagaaccatt gggtggcaga aaacgcatcg ccagaagcag ggcgataata cccaacggta
    14521 cgttgattaa aaataaccat ttccaggatg cgatggagag gattgctgca gcaattgtcg
    14581 gcccggcagc agaagagacg gcaacaataa acgagtttat gcccatccct ctacccagaa
    14641 aacgttgtgg atagatcagg cggataagtg cggtattaac gctcatcaac gctgcaccgc
    14701 cgaaaccttg tatgacacgc gcaagggtga gcatttgcag cgaatcagaa agggcgcaga
    14761 acagtgaaga caacagaaaa acgaccagac cgcatttata aatacgtcga tagccaaaca
    14821 tatcgcccag aaacgaaaac gagagcaggg agatgacaat ggcgatttga taggcgttca
    14881 ctacccagat ggaactggcc ggcgtggcat gaaggtccgt agcgattgtt ggcaaggcga
    14941 cgttggcgat tgcgccgtca aggacagcca tcgaaatacc aatcacaatg gttaatatcg
    15001 caccgtatcg ctggggcaat ggcaggccgt cggcctgaac ttttggcata ggaattttat
    15061 atctttggtg aataatcaac aggggtatga ttctagcact gatattcacg gtggatgtcg
    15121 cagaattgta actaaccggc aaaagatgga ttgcgaccac cctggtgcga aattataata
    15181 aaaaacagtt ctgattttta taaaacactc gcaatgaggt gataaatggc taacgcagat
    15241 ctggataaac agcctgattc tgtatcttcc gtgctaaaag tttttggcat tttgcaggcg
    15301 ctgggtgaag agcgcgaaat agggataacc gagttgtcgc agcgcgttat gatgtcaaaa
    15361 agcaccgttt atcgcttttt acagaccatg aaaaccttag gttatgtggc gcaggaaggg
    15421 gagtcggaga aatattcgct gaccctgaaa ttgtttgaac tgggcgctcg cgcgttgcaa
    15481 aacgtcgatt taattcgtag cgcagatatc cagatgcgtg agatctcccg cctgaccaaa
    15541 gaaactatcc acctcggcgc actggacgaa gatagtattg tttacatcca caaaattgac
    15601 tcaatgtaca atttgcgcat gtattcacgg attgggcgtc gtaatccgct gtacagcacc
    15661 gcgattggta aggtactgct ggcatggcgc gatcgcgatg aagtgatgca aattcttgag
    15721 ggcgtggagt ataaacgcag taccgagcgg accatcacca gtacagaagc gttattaccc
    15781 gttctggacc aggtgcgcga gcaggggtat ggcgaagata atgaagagca ggaagaagga
    15841 ctgcgttgca ttgcggttcc ggtatttgat cgctttggcg tggtcattgc cggtctgagc
    15901 atctccttcc cgacgttgcg tttctctgaa gagcgtttac aggaatatgt cgcaatgttg
    15961 cataccgcgg cgcgcaaaat ttctgcccaa atgggttatc acgactatcc gttctgagga
    16021 aagtaagaac ctgtctgaat atcaaacaga caggttcttt atttagcatg agaaaaataa
    16081 agttgaaggt ggcgttatat taaacgcact tgcgataaga atattttact catgagtgag
    16141 aatctggtta tttattatcc ttaaccatta tcgaccacaa tattgctttt acgtaacaac
    16201 gggcaatctg ttatccccag aaaaccactt ttagtgtgca ggtattgtac cgtgctggtg
    16261 cctctggcgg tcagataggt acattgtaaa cctaatcccg cggcattctc tttgcttcca
    16321 atcaaaacgc catatccgct gagtaataat cctatccata ccagcgctat cagcataact
    16381 gtgcgaatga tgaatcgcat tacaacctct tctcttttta tgttcgctta atcgtagcgg
    16441 caatatgcgc tgaagcaagc gactcattcc gaaaaagcac gaatatcgac atagttaggc
    16501 gctgtttaac taacgcatgc tagtttaatg acataaggta ggtgaaacgg agattggagt
    16561 gaaaaagttt cgatgggtcg ctctggttgt cgtggtgttg gcttgcttgc tgctttgggc
    16621 gcaggtattc aacatgatgt gcgatcagga tgtacaattt ttcagcggaa tttgtgccat
    16681 taaccagttt atcccgtggt gatatcattt tttcagcact agattttctt cctctccagc
    16741 agtggtagaa tagcgcctct actcagaggt ggtgaaatga acgaagttgt aaattcaggc
    16801 gtgatgaaca ttgcgtcttt ggttgtatcg gtggtggttc ttcttatcgg gctcatcttg
    16861 tggtttttta tcaatcgtgc cagttcacgg actaacgaac agattgaact gcttgaggcg
    16921 ttgctggatc agcaaaaacg tcaaaatgca ctgttacgtc gtttgtgcga agcaaacgaa
    16981 cctgagaaag cagataaaaa gaccgttgag agtcaaaaat cggttgaaga cgaagatatt
    17041 attcgtctgg tcgccgaacg ataatccttc ctcaatcttg agtaaccccg tcgttgaagg
    17101 gttactcaat tcatgctgat tatacgcact gtcatggtgc agctgcttca aattaaaact
    17161 ctggtctgac ttcaaataat tatcatatta ttaatttgcg taa
//
LOCUS       DADTFG010000106        16932 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.107, whole genome shotgun sequence.
ACCESSION   DADTFG010000106 DADTFG010000000
VERSION     DADTFG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.107"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(132..470)
                     /locus_tag="J1138_003237"
     CDS             complement(132..470)
                     /locus_tag="J1138_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBA8455000.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPGGGEYWRLKYRV
                     AGKEKLLALGVYPEVTLADAPAKLEEAKRGISGGIDLMEVKREEKIARETQLNNTFKD
                     IALEWHSNKL"
     gene            complement(625..700)
                     /locus_tag="J1138_003238"
     tRNA            complement(625..700)
                     /locus_tag="J1138_003238"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(665..667),aa:Thr,seq:cgt)
     gene            complement(815..2068)
                     /gene="proA"
                     /locus_tag="J1138_003239"
     CDS             complement(815..2068)
                     /gene="proA"
                     /locus_tag="J1138_003239"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBA8455001.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADACANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2080..3183)
                     /gene="proB"
                     /locus_tag="J1138_003240"
     CDS             complement(2080..3183)
                     /gene="proB"
                     /locus_tag="J1138_003240"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBA8455002.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            3472..4527
                     /gene="phoE"
                     /locus_tag="J1138_003241"
     CDS             3472..4527
                     /gene="phoE"
                     /locus_tag="J1138_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBA8455003.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMMPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(4566..4967)
                     /locus_tag="J1138_003242"
     CDS             complement(4566..4967)
                     /locus_tag="J1138_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBA8455004.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAKVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5025..6275)
                     /gene="frsA"
                     /locus_tag="J1138_003243"
     CDS             complement(5025..6275)
                     /gene="frsA"
                     /locus_tag="J1138_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBA8455005.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPGPFPTVLMCGGLDAMQTDYYSLYER
                     YFAPRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRF
                     GANVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASD
                     EALRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIP
                     FNPVYRNFDKGLQEITDWIEKRLC"
     gene            complement(6367..6825)
                     /gene="gpt"
                     /locus_tag="J1138_003244"
     CDS             complement(6367..6825)
                     /gene="gpt"
                     /locus_tag="J1138_003244"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBA8455006.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7086..8543
                     /gene="pepD"
                     /locus_tag="J1138_003245"
     CDS             7086..8543
                     /gene="pepD"
                     /locus_tag="J1138_003245"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBA8455007.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(8600..9214)
                     /locus_tag="J1138_003246"
     CDS             complement(8600..9214)
                     /locus_tag="J1138_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBA8455008.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLEIET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9211..10362)
                     /locus_tag="J1138_003247"
     CDS             complement(9211..10362)
                     /locus_tag="J1138_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBA8455009.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAALFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(10541..10993)
                     /locus_tag="J1138_003248"
     CDS             complement(10541..10993)
                     /locus_tag="J1138_003248"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA8455010.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(11003..11401)
                     /gene="yafO"
                     /locus_tag="J1138_003249"
     CDS             complement(11003..11401)
                     /gene="yafO"
                     /locus_tag="J1138_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBA8455011.1"
                     /translation="MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDAAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKVAEAFRMRF"
     gene            complement(11404..11697)
                     /gene="yafN"
                     /locus_tag="J1138_003250"
     CDS             complement(11404..11697)
                     /gene="yafN"
                     /locus_tag="J1138_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBA8455012.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(11749..12804)
                     /gene="dinB"
                     /locus_tag="J1138_003251"
     CDS             complement(11749..12804)
                     /gene="dinB"
                     /locus_tag="J1138_003251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBA8455013.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12875..13660)
                     /gene="lafU"
                     /locus_tag="J1138_003252"
     CDS             complement(12875..13660)
                     /gene="lafU"
                     /locus_tag="J1138_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBA8455014.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PSKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            13605..15344
                     /gene="flhA"
                     /locus_tag="J1138_003253"
     CDS             13605..15344
                     /gene="flhA"
                     /locus_tag="J1138_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HBA8455015.1"
                     /translation="MLSRSDLLTLLTINFIVVTKGAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRN
                     QLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALS"
     gene            15449..15727
                     /locus_tag="J1138_003254"
     CDS             15449..15727
                     /locus_tag="J1138_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBA8455016.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            15720..16076
                     /locus_tag="J1138_003255"
     CDS             15720..16076
                     /locus_tag="J1138_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBA8455017.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIIDVLEREFSAQM"
     gene            complement(16133..16906)
                     /locus_tag="J1138_003256"
     CDS             complement(16133..16906)
                     /locus_tag="J1138_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBA8455018.1"
                     /translation="MHDQPLLRMPLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         4168 a         4382 c         4209 g         4173 t
ORIGIN      
        1 atataccatt ggaagattta gaaaagcatg tccaggagtt taaggacaaa tctcatactg
       61 attatcggaa gcttttatca ttatatgatt accaagcata caatgtataa ttatatacac
      121 tattattgtg attataattt gttactgtgc cactcaaggg caatatcttt gaaggtgttg
      181 tttaactgcg tttcccgggc aatcttttcc tctcgcttca cttccatcag atcgattccc
      241 ccagagatac ctcttttagc ttcttcaagt tttgcaggag catcagctaa ggtgacctca
      301 ggatacacac ctagtgctaa cagcttctct ttaccagcta cacgatactt gagacgccaa
      361 tattctcctc caccaggttt taccaggagg tacagaccac caccatcagc caacttgtaa
      421 gccttctctt ttggcttggc agtgtctatt tgacgggcat tgagtttcac ttgggggtat
      481 ctccactaaa ctgaacagca aatcccctca attgcttgta gagtttgggg tacttaaata
      541 gacgtcaaaa gactaaaagg ggcattaata tactgattat aagaggtttt taaatacttg
      601 agtagacttg gggagacgtt agaatggtgc cgataatagg agtcgaacct acgaccttcg
      661 cattacgaat gcgctgctct accaactgag ctatatcggc cctgaaagga catgttcacg
      721 aacgtgaatc acggtggaca aggttaaaac taaccgggcg atgcgtcaat ggccttgtga
      781 atcaaatggc tacttttgca tcacccggtt ttatttacgc acgaatggtg taatcaccaa
      841 tgccgatcca cttataagtg gtcagagctt ccagccccat tgggccgcgc gcgtggagtt
      901 tttgtgtgct taccgcaact tccgccccca gtccaaactg gccgccgtcg gtaaaacgcg
      961 tagaggcgtt aacgtaaaca gcggaggaat ccacttcgtt aacaaaacgc tgggcgttgc
     1021 gcatatcgcg ggtcaggatc gcatcggagt gttgcgtgcc gtgttcacga atatgggcaa
     1081 tggcgtcatc cagatcgcta acgattttga cgttcaaatc taatgacaga aactcatcgt
     1141 catactcttc ggctttaaca gccaccacct tcgcggggcc tgcctgcaac tgcgccagcg
     1201 cggctgcatc agcgtgtaac gtcacgccgc tttccgccat ttgtttgctt aatgcgggca
     1261 ggaagctatc agcgatgttt ttattcacca gcaacgtttc taccgtatta catgtgctcg
     1321 gacgctgagt tttcgcgttg acaattactt tcagggcttc agcgatctct gcactttcat
     1381 caacgtaaat atggcatacg cctataccgc ctgtgatcac cgggattgtc gactgctcgc
     1441 ggcacagttt atgcaaacca gccccaccgc gcgggatcag catgtcgatg tatttatcca
     1501 tacgcagcat ttcactgacc agcgcacggt caggattatc aattgcctgc acggcacccg
     1561 ccggtaagcc gcaggatttc agggcgtcct gaatcaccgc taccgttgca gcgttagtgc
     1621 gacaggtttc tttgccgccg cgcaggatca ccgcgttacc ggttttcagg cacagggaag
     1681 caacatcaac cgtcacgttc gggcgcgctt cataaatcac gccaatcacc cccagcggta
     1741 cgcgacgacg ctcaagacgc aggccgctgt ccagtacgcc gccatcgatt acctgcccca
     1801 ccggatcggc gagattgcac acctggcgca catcgtcggc aatgcctttc agccgtgcgg
     1861 gcgtcagtgc cagacggtca agcatcgctt cgctaaggcc attggcacac gcgtctgcaa
     1921 catcctgcgc gttagcgttg aggatacttt cgctttgtgc ttccagttca tcggcgattt
     1981 tttccagcac acgatttttt tcgcggctgg agagttgcgc taatttatac gaggcttgct
     2041 tcgcggcaat gcccatttgt tccagcatcg gccagctcct taacgggtaa tcatgtcatc
     2101 acggtgaacg gcaaccgggc cgtattcata tcccagtatt gcatcaattt cttgcgagtg
     2161 gtgtccggca atacggcgta atgcatcgct gttgtaacga ctgacgccgt gggcgatatc
     2221 gcgaccttcg aggttgcaaa tacggatgac ttcaccacgc gagaaattgc cagtcacgct
     2281 tttaatgcct ttcggcaaca gggagctgcc gcgttccaga atggcggcag ttgccccttc
     2341 atctaccgtg atttcacccg caggcggcgc accgaaaatc cagcgtttac ggttttcaag
     2401 cggagtcgcc tgggcatgga acagcgtacc gacggaaatg ccttccatca catcaccaat
     2461 aacgcccggc ttgctgcccg cggcaataat ggtgtcgata cccgcacggc aagccacgtc
     2521 agcggcctgc aatttggtgc tcataccgcc agttccgagg cctgaaacgc tatcaccggc
     2581 aatcgcgcgc agtgcgtcat caatgccgta cacatcttta atcagttctg cctgcggatt
     2641 gctgcgcggg tcagcggtat acaaaccttt ttgatcggtc agcagcaaca gtttatcggc
     2701 acccgccaga atcgccgcca gcgcagaaag gttatcgtta tcaccgacct taatctctgc
     2761 agtagcgaca gcatcgttct cattaattac cggaacgata ttgttatcga gcaacgcacg
     2821 cagggtatcg cgggcgttca ggaagcgttc acgatcttcc atatcagcac gggtcagcaa
     2881 catctgcccg acatgaatgc cgtaaattga aaacagctgt tcccacagtt gaatcagtcg
     2941 actctgccct accgccgcca gcagctgttt tgaagcgata gtcgctggca attccgggta
     3001 ccccaggtgc tcacgtccgg cggcaatcgc acctgacgtc acaataacaa tccgatgccc
     3061 ggcggcatgt aactgcgcgc actggcgaac aagttcaacg atatgggcac ggttcaggcg
     3121 gcgcgatccg cctgttagca cactggtgcc gagttttacc accagcgtct ggctgtcact
     3181 catgattctc tgccattcaa ttgtaggaaa aaatgatatc aaacgaacgt tttagcagga
     3241 ctgtcgtcgg ttgccaacca tctgcgagca aagcatggcg ttttgttgcg cgggatcagc
     3301 aagcgtagcg gcagttgttt acgcttttat tacagattta ataaattacc acattttaag
     3361 aatattatta atctgtaata tatctttaac aatctcaggt aaaaaacttt cctgttttca
     3421 acgggactct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
     3481 agcactctgg cattagtggt gatgggcatt gtggcatctg tatccgtaca ggccgcagaa
     3541 atatataaca aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
     3601 atgagtgata atgacagtaa agatggcgac cagagttata tccgttttgg ttttaaaggc
     3661 gaaacacaaa ttaacgatca actgactggt tatggtcgtt gggaagctga gtttgccggt
     3721 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
     3781 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
     3841 gcctggaccg atatgttccc ggaatttggt ggcgactcct cggcgcagac cgacaacttt
     3901 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
     3961 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
     4021 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggtggcag cgatttcgcc
     4081 atcagtggag cctataccaa ctctgatcgt accaacgagc agaacctgca aagccgtggc
     4141 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
     4201 ctggcaactt tctattctga aacacgtaaa atgatgccaa tctctggcgg ctttgccaat
     4261 aagacgcaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
     4321 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     4381 aattatgtcg atgttggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     4441 tataaaatca accaactgga tagcgataac aaattgaata ttaataatga tgatattgtc
     4501 gcggttggca tgacctatca gttttaatgg atattgccgg atgtgatgca tccggcacat
     4561 ttcattcacg ccgttaactt caccggttca tcacgaaaat catctgccgg ttccagcttc
     4621 agattcagcg tcgccagcag ctcacgcagc ttctcgtgaa attcacgcag ggtgtgctcc
     4681 agtcgttcaa ccactttggc atcttttatc tgaacactcg tccagtcgcc tgctttatcg
     4741 aacagaccaa actggtaact gtaggtaaaa cgggattcct gcgcttccag ttccatccac
     4801 cagccccaga actcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     4861 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     4921 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc aatctcctgt
     4981 tgtgatgcaa ctgttttacc aaattggtaa aatttagcaa atttttaaca caaacgtttt
     5041 tcgatccagt ctgtgatttc ctgaaggcct ttgtcaaaat tccgatacac cgggttaaat
     5101 gggatctcta acaatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     5161 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgtccc
     5221 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgcaacgc ttcgtcggaa
     5281 gcatcatgca tccccaaacg gctcgccaga acgtcaagat acatttccgg cacctgttgc
     5341 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     5401 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     5461 aaggccgcga cgcgagtgtg atctacccac ggtacgttag gcagcgcctt taagacgtgc
     5521 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     5581 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     5641 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccaggg
     5701 ccatcgcctt tcggcatatg caaaaagccg gtgatgggcg caccgccggg tacggtaaac
     5761 tccatctgcc gcatcgtgcc cggcagacgc tgagcggcct cttcataggc gcggtttgac
     5821 aaagcctgcg cttgctcggc caggtcatct cctttcagat gaggataggc agcaatgttg
     5881 tacaacgtag ccgcatgcag ccagtgtcga ccgctgagtt gcggatcttc ttccgcacag
     5941 gctttttgtt gccacaccat cgcctgggta gcccactcat aaatccagtt gccgccgcgg
     6001 tagccaatca ccgtatcgta taaatcatcg tcggtacgtt cggcatcgct catcacaata
     6061 cgcgcctgga catcgaggat ttcacgtggg tcaatgccgc gccagatcca catcagacgg
     6121 ttaatcatgc gataccagtg agggatggtt ttaccatcaa gggccgactg cacaggcagt
     6181 tgtgcgccgt gactaaagcg gcggactagc gtcgaggttt caggatgttt aaagcggggt
     6241 ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg cagcctccag aatacttact
     6301 ggaaactatt gtaacccgcc tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg
     6361 aaaagattag cgaccggaga ttggcgggac gaatacgacg cccatatccc acggctgttc
     6421 gatccaggta ttttgcggaa tatcaacaac ataatcatca accagcggac gaccggccgg
     6481 ttttgcgaag atggtgacaa agtgcgcttt tggatacatt tcacgaatcg caaccgcagt
     6541 accaccggta tctaccaggt catcaataac gatgaagcct tcgccatcgc cttctgcgcg
     6601 tttcagcact ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac aaacggtatc
     6661 gacatgacga atacccagtt cacgcgccag taacgcaccc ggtaccagac cgccacggct
     6721 tacggcaata atgcctttcc attgttcaga aggcatcagt cggcttgcga gtttacgtgc
     6781 atggatctgc aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa gtgtcccagc
     6841 ctgtttatct acggcttaaa aagtgttcga ggggaaaata ggttgcgcga gattatagag
     6901 atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg cactgcctct
     6961 ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc ttgtgttgac
     7021 aacattttct gctaaccctg tgacctgcaa tactgttttg cgggtgatcg acaaggagac
     7081 ttaacgtgtc tgaactgtct caattatctc cacagccgct gtgggatatt tttgccaaaa
     7141 tctgttctat tcctcacccg tcctatcatg aagagcaact cgctgaatac attgttggtt
     7201 gggcaaaaga gaaaggtttc catgtcgaac gcgatcaggt aggtaatatc ctgattcgta
     7261 aacctgctac cgcaggtatg gaaaatcgta aaccggtcgt cttgcaggcc cacctcgata
     7321 tggtgccgca aaaaaacaac gacaccgtgc atgacttcac taaagatcct atccagcctt
     7381 atattgatgg cgaatgggtt aaagcacgcg gcaccacgct gggtgcggat aacggcattg
     7441 gtatggcctc tgcactggcg gttctggctg acgaaaacgt ggttcacggc ccgctggaag
     7501 tgctgctgac catgaccgaa gaagccggta tggacggtgc ttttggctta cagagcaact
     7561 ggttgcaggc tgatattctg attaacaccg actccgaaga agaaggtgaa atctacatgg
     7621 gttgtgcggg aggtatcgac ttcacctcca acctgcattt agatcgtgaa gcggttccag
     7681 ctggttttga aaccttcaag ttaaccttaa aaggtctgaa aggcggtcac tccggcggtg
     7741 aaatccacgt tggcctgggt aatgccaaca aactgttggt gcgcttcctg gcgggtcatg
     7801 cagaagaact ggacctgcgt cttattgatt tcaacggcgg cacactgcgt aacgccatcc
     7861 cgcgtgaagc ctttgcgacc attgctgtcg cagctgataa agtcgacgcc ctgaaatctc
     7921 tggtggatac ctatcaggag atcctgaaaa acgaactggc agagaaagag aaaaatctgg
     7981 cattgctgct ggactctgtt gcgaacgata aagctgccct gattgcgaaa tctcgcgata
     8041 cctttattcg tctgctgaac gccaccccga acggtgtgat ccgcaactcc gacgtggcaa
     8101 aaggtgtggt cgaaacctcc ctgaacgtcg gtgtggtgac catgaccgac aataacgtag
     8161 aaattcactg cctgatccgt tctctgatcg acagcggtaa agactacgta gtgagcatgc
     8221 tggattcact gggtaaactg gcaggtgcga aaacagaagc aaaaggcgca tatcctggct
     8281 ggcagccgga cgctaactct ccggtaatgc atctggtacg tgaaacctat cagcgtctgt
     8341 tcaacaaaac gccgaacatc cagattatcc acgcgggcct ggaatgtggt ctgttcaaaa
     8401 aaccgtatcc ggaaatggac atggtgtcta tcgggccaac catcaccggt ccacactctc
     8461 cggatgagca agttcatatc gaaagcgttg gccattactg gacactgctg actgaactgc
     8521 tgaaagaaat tccggcgaag taattatttg atttgctgcc ggatggcgtt taatcgcctt
     8581 ccggcagttt catccttcat tatccttcga taaaagccat ccctgtgaac gttcgtcgcg
     8641 ggttgcctcg ttcaatctgg tgatggaaca ttcgccgctg cgatttcagc gccgcgctat
     8701 tttcctgttg ctgttgttcc agcttccagg caatcagtaa tcgcgccagc cgtttgttag
     8761 catgctgact gcgctctgac tgaaccttca cgctaatacc ggatgccaga tgcgtggcgc
     8821 gtaccgccga gtcggtttta ttgacatgtt gaccgcccgg ccctgacgaa cgcagcgtct
     8881 cataacggat tgcatccgat tgttcctgct catcagcggt aaaacgccca atgcccagaa
     8941 accagttttt gcgcccatga tgaggccgat acggactcgg acaaatccac tggatagtgc
     9001 cgcaccagct ttcgcttaga gcccatgcgt tatcgccatc cagagaaatc agcgccgaac
     9061 gcagtgtgtc agagtagcga cccgtttcta tttccagcac cgttaccgcg acgtcctgtc
     9121 gggtagcttc tttaatcagc ctgtccagtg cttttttcac tgcgagacaa cattcttccg
     9181 gcccctgagc agaggagagt tgtagcaaga tcatcctttt ttccctccac tgtttttgag
     9241 cgtcagcacc gggcgcagtc gcgcgacagg aataattaac ccagccagca ccagacattg
     9301 caccacgctt tcagccgatt tataggcttg tggcgcttct tcaaagatga gttgtttatc
     9361 gcgacaaatt acccgactgc caagttcagt ccgcgagagt tgcgtcgccg tgtatttcgc
     9421 tgccagacgc cctttacact cggtgcgccc ccatttacgc cccgccccat gcgccagcga
     9481 gtggagcgtt ttttcgttcg caacaggttt aaccagccag gagtaatcgc cgcgtgaacc
     9541 gggaataatc accagaccgt tgtcatccgg cgtggcgcct ttacgatgca accagccctg
     9601 ttgatcgccg atttggcacg cgctaacaaa gttatgcgcc acatccagaa ccggacttcc
     9661 ggtggcctta acttgttgca ttatgcgcaa agcgatcagc tggcgattaa tacgcgcaaa
     9721 cgccagcgca tcgtcatgtt ccgcaatata ccgtagcgcg tcgtcactgc cttcaggcaa
     9781 accatgatgc gaaaacgagg caatatgccg ctgtaaaata gactgcccca atcccctcga
     9841 gccgctatga accagcagtt gcagatgctg cgcatccaga ccagccagtg caaacagttc
     9901 cgcgtcgata atttgatcaa tctgttgcag ttctgcgaag tggttaccgc caccgatgga
     9961 gccaagcgag ttgcgccacg gatgctgtgc aaatgctgac ggcaggtttt cctccagcca
    10021 gctttcttca gcaacgtcat ccagatctga taatcgcttt tcaaacttat cggcgttgta
    10081 tttgcgagcg agaatatctg tttgccatag cgccatgccg cagccgatat cattgccgac
    10141 cagtgccggg taaaaacgac caacagagaa taacgctgcg ccaatcgggt agccgcgtcc
    10201 ggggtgtaaa tctggcatcc ctactacgcg ctgcatgttg ggtaaatttg ccgtggtgtg
    10261 taattgttgg atcgctaaac tctcgatcca caggtcatca gatgcgatgg taaataccgc
    10321 atcggataag ggacgaatat atttgcccat gtaccattcc atttatgaat aaaaatcagg
    10381 aaatggcaga caaatccagg gcaagaaaaa aagaattaat cctggaaagg tctgcaaaga
    10441 gtcattgata aaggatttgt catgatttac ctccttgcag tttgtggaat aagaaaacgc
    10501 gcacatacta gacgggcgtt attttcattg caagctggac ttaatgttgc ggtttatatc
    10561 gcatataaaa attagtaaac cacgttcccc ggcattcaac gcgctgctgc ttaactgtct
    10621 gaaaaccata acgttcaaaa aagggctttg cggttatgct ggcgtccacc gtaagttcgg
    10681 attcagactt aatcaatggt tttaacaaag cgctggcaac cccgcgtcgg gtgtattccg
    10741 gttcaacaaa taacatatcg atataatgtc caatgcaggt aataaaacca accggttgtg
    10801 cattaataac tgcaacccgc acttgtgatt tcgcgagttt ctccttccag cgagattcgt
    10861 caatctgcgc ccaggcggca atttgctgtg gagaatagtg ctgactcgcc gtcatcgtaa
    10921 cagctcgaag gaaaatagcg catagttgct gaaaatcacc aggctgatag tttcttattt
    10981 gtatgttatt cataatataa attcaaaaac gcatgcgaaa cgcttctgcc actttcccaa
    11041 ttttatgcat ttggttgtta tctcgagcca gtttatgagg ttcaggtttc agaatggcaa
    11101 tgagcaacca tgcttgctca tcaaacgcgc cctgacaata taccaaatgc gctgcgtcat
    11161 tcgttctgct gaattggcgc aactgtggcg gaaatggatt attcacattt gccagatgaa
    11221 tatgagcaac tcgctcaaat ttgattaatg gccaggtaaa cgagtcgtcg tagagtgcat
    11281 cacgaccaaa tatatctggc aaaacaccgt cacgcttata ggaaataaaa tccgccgtta
    11341 acgcttcaag ttcctctgct gtaagttgca ggcgaataag ttttgttttg aatacccgca
    11401 tccttattcc ttaaagtcat tgaaatcatc atccgtcata tcattaagat attgttcagc
    11461 gcggcgtgta atttcctcta atcttgcagc actcggacgg aagcgcgcct ttatgggctt
    11521 tttctcctct tgttctgcaa gcatgtccat taatgcttca aatgcgctgg cacttaagag
    11581 atatcctgcg gggcgattat tagaaagaac cgcaacaggt tgatcaataa agtatttagc
    11641 tgggttttta cgtaactcag tgatattgac cgatttttca gcgagaattc gatgcataca
    11701 gtgatctcct cataataatg cacaccagaa tatacaaaat agtatacatc ataatcccag
    11761 caccagttgt ctttccattt gcgggtcaag caacgtcaca tgcagcccca ccagtcgcac
    11821 accgcgcccg ccgcggcgtt catcccaagt tttacgcgcg gtggcgatta gatcagattt
    11881 attcagccgc ggccagacgt gttcctgggt ggtttgctga aaatcgtcga actttaattt
    11941 caccccctgg cgggcaatca gcaaatcagg ttttaccttt gccaaacgac gctccagttc
    12001 agggtaaagc cgctcgatga tcgcttcaca ttcagaccag tgatgaatat cttccgccat
    12061 cgtgcgttcc acgccgacgg attttcgcaa ccgttcgctg ttaacatcgc gttcgtcaat
    12121 gccctgacta cgctcccaca aaatgcggcc aaatttgcca aaacgtttga gcagagtcac
    12181 cagatcacac ttttgcacat caccgcaggt tcgtagcccc atcgcttcca gtttagccgc
    12241 tgagactttg ccgacgccgg gaatttttgc cagcggtaag gtttgcagaa atgccggaac
    12301 ctcagccgga gtaatcacaa actgaccgtt gggtttattc atgtcggagg cgattttggc
    12361 gagaaacttt actggtgcca cgcccgcaga cgccgtcagt tgcagctcgt tgaagatcgt
    12421 ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg caatggacgc tatcggtgac
    12481 atcgagatag gcttcatcca gtgataacgg ttcaatgcgc gaggtatagc gcgagaaaat
    12541 ttcgcggata tgatttgagg cttctttgta ggcgtcaaag cgccctggaa gcaaggtgag
    12601 atgcgggcat aatttgagcg ccatccctgt cggcatagcg ctacgtacgc caaatttacg
    12661 cgcgggataa ttggcggtgc tgatcacccc ccgtcgttcg cggctgccgc caatagcaat
    12721 agggatatcg cgcagggcgg gattatcgcg catctccacc gctgcgaaaa agcagtccat
    12781 atccacatga atgattttac gcattgctca cctctcaaca ctggtaaagt atacagtgat
    12841 ttcaggggtt gagaaatgcg taaagattca gcatttaacg cgctcgctgc gaaagcacct
    12901 gctgcttatc cagcttttgc accagtggct gcaccacttt atccccatgc tgaccaaagt
    12961 attgatacag cgtatcggac gcacttttgg tcagcaccat aatctcaatg cgccggttgc
    13021 ccgcgctttg cggatttttg gcatccagca acatctggtc cgccattgcg cttacctgca
    13081 tcactttatc ttccggcatt ccggcctctt ccagcacccg acgagccgaa agcgcgcgat
    13141 cgcccgaaag gttccagttg ttgtagatat tgtttttgta ggccatcgca tcggtatgcc
    13201 cggtaataat aattttgtta tcgagcgagt cgaacactgg cgcaagctcc accagcaaag
    13261 ttttaaagaa cggcataatc ttcgcgctgc cgcgttcaaa catattgcgg ttctggtcgt
    13321 ctttaatcag cacgcgtaat ccctggggaa caatctccat ctccagattc gcttccatat
    13381 gcgcatcacg ggcgatggta ttgatactgg ttgccagctc gccaagctcg gtggcggatt
    13441 tcttcttcaa cagcgccgtt ttctcgttga cgtcgcgcgc ttttttctcc gtctcttccg
    13501 gcaccgccac ggtggcaggt tttgacggat gagacgggct gattttattt aacggcgaca
    13561 gcccgccacc attgaaaatc gactgcccgt gcagcgcggc gataatgctt tcacgctcgg
    13621 atttactgac gctgttgaca atcaacttta tcgtcgtcac caagggggcc gagcgtattt
    13681 ccgaggtttc tgcccgcttt accctggacg cgatgcccgg caaacagatg gcgattgacg
    13741 ccgaccttaa cgccgggttg atcaatcagg cgcaggcgca gacccggcgt aaagatgtcg
    13801 ccagcgaggc cgatttctac ggcgcaatgg acggggcatc aaaatttgtg cgcggggacg
    13861 ccatcgccgg gatgatgatt ctggcgatca acctgatcgg cggcgtctgt atcggcatct
    13921 tcaaatacaa cctgagcgcc gacgccgcct tccagcaata tgtgctgatg actatcggcg
    13981 atgggctggt ggcgcaaatc ccttccctgc tgctctccac cgcggcggcg attatcgtca
    14041 cccgcgtcag cgataacggt gatatcgccc atgacgtgcg taatcagttg ctggcaagcc
    14101 cgtcggttct ctacaccgcc actggaatta tgttcgtgct ggcggtggtg ccgggaatgc
    14161 cgcatttccc gttcctgatg ttcagcgccc tgcttggttt taccggctgg cggatgagca
    14221 agcgcccgca agcggcggag gcggaagaga aaagcctcga aacgctaacc cgtacgatga
    14281 cagaaaccag cgagcaacag gtcagttggg aaactattcc gctgatcgaa cctatcagtt
    14341 taagcctcgg ctacaaactg gtggcgctgg tggataaagc gcagggcaac ccgctcaccc
    14401 agcgtattcg cggcgtacgt caggtgattt ccgacggcaa cggcgtgctg ctgccggaga
    14461 tccgtattcg ggaaaacttc cgcctcaagc ccagccagta cgctattttc atcaacggca
    14521 ttaaggctga tgaagcggat attccggcgg ataaactgat ggccctgccc tccagcgaaa
    14581 cctacggcga gatagacggc gtgctgggga acgacccggc gtacgggatg ccggtcacct
    14641 ggattcagcc cgcgcagaaa gcgaaggcgc tgaatatggg gtatcaggtg atcgacagcg
    14701 ccagcgtaat tgccacccat gtgaataaga ttgtgcgcag ctatattcct gatttgttta
    14761 actatgacga tattactcag ctgcataacc gtttgtcgtc gatggcaccg cgactggcgg
    14821 aagatttgag cgcggcgctc aattacagcc agttgctgaa agtgtaccgg gcgctactga
    14881 ctgaaggcgt ttccctgcgc gatatcgtca ccatcgccac cgtgttggtc gccagtagtg
    14941 cggtgactaa agatcatatt ctgctggcgg ccgatgtgcg tctggcgctg cggcgcagca
    15001 ttacccatcc gttcgttcgc aagcaggagc tgacggtgta tacgctgaat aatgagctgg
    15061 aaaatctgct gaccaacgtg gtgaatcagg cgcaacaggc cggcaaagtg atgctcgaca
    15121 gcgtgccggt agacccgaat atgctcaacc agttccagag tacgatgccg caggtgaagg
    15181 agcagatgaa agcggcgggg aaagaccctg tgctgctggt gccaccgcag ttgcgccctt
    15241 tgctggcgcg ctatgcgaga ttgtttgcgc cggggctgca tgtgctgtcg tataacgaag
    15301 tgccggacga gctggagttg aagattatgg gggcgttgag ttgattgagg aatcttttcc
    15361 atgctttgca gcacctgaca ccattaattg acgtcatact gcaaagagac tacgatgaca
    15421 ccatacaatg acgtcatgga ttgatagtat gattaaggtc agcgcgctag gctctcgtca
    15481 tagaaaaaca ttcgacgatg taatgacgga gccgccaaaa tctggaatta aatgggatga
    15541 tgttgttgca ctcattaaag ctgtgggtgg aacaataaaa aataacaacg gctccagacg
    15601 aaaatttcag attggaacga ccaaattcag cacacatgag ccacatccaa agaatgtgat
    15661 ggataaaggc gcagtagcag gtcttaagga gtggtttgtt aactgtgtag gggtagatta
    15721 tgaataaagc atcgaacatt atcacaatta aaggtcaacc agccgcagtg acatttgaag
    15781 ccgatatcaa cgcatttcgc ggcaagttcc tcaatgtaaa cggttattgt gactttgtag
    15841 ccacaagtat tgaggcatta tatcgtgaag gcgaaagcgc gctggatgag tggatggcgg
    15901 attgtgaaga ggacggtatt gccccttttc gtgaagaaga agagcaaaaa cgcctgacgc
    15961 ttcgtgttcc tcatcacatt gattcgcggc tgaccatcgt cgccagacaa cattccattt
    16021 caaaaaacca gctcattatt gatgtcttag aacgtgagtt ttcagcccag atgtaaacag
    16081 gtcggatgcc gcgtggcatc cgaccttttg ccagttcgtc aatattaccc atttacaaaa
    16141 tcgtctcttc cgtcaaaatc ctgcgcgcgc ccaaaaagtg gtcctgccag aaaggttcgg
    16201 ctaaccggct tacccgtatg gtttccccgg tgcgtggcga ttcgataaat tgcccgtctc
    16261 ccagatacac gcccatatga tcggcaatct cacggctgtg aatatggaaa aacagcaaat
    16321 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg atggtagtga tacatctcat
    16381 tggccgtacg cgggagcttc gcctcgagga tcttgttgta ggcataaaaa accaacccgc
    16441 tacagtcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggctta ccaagctgct
    16501 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    16561 ggttgttttc cgccagcgcc tgccagcgtc tatcactcgc cttgagcggc gcaggaaacc
    16621 attctggatg ctgttttatc tgctcccggt tgtgctggcg cagcgccctt ctgctttcgg
    16681 cgttgccttc cacaatgtag ctggcctgct ttttcagatg ggtttggtac tgttttaata
    16741 aacgcgcacg gttttgcatc cgctggcggg cggcgtagct gaaagagata tgtgatgtgg
    16801 tggcgctggc aaatgaagaa aacggcaagc aaagcaacaa taatcccgac aacacaaaag
    16861 agggaatcga cggcaaaggc attctcaata atggctggtc atgcattgat gttgcagtgt
    16921 atcccggccc gt
//
LOCUS       DADTFG010000107        16883 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.108, whole genome shotgun sequence.
ACCESSION   DADTFG010000107 DADTFG010000000
VERSION     DADTFG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.108"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            247..456
                     /gene="mokC"
                     /locus_tag="J1138_003257"
     CDS             247..456
                     /gene="mokC"
                     /locus_tag="J1138_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBA8455019.1"
                     /translation="MQNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(561..1691)
                     /gene="dnaJ"
                     /locus_tag="J1138_003258"
     CDS             complement(561..1691)
                     /gene="dnaJ"
                     /locus_tag="J1138_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBA8455020.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1780..3696)
                     /gene="dnaK"
                     /locus_tag="J1138_003259"
     CDS             complement(1780..3696)
                     /gene="dnaK"
                     /locus_tag="J1138_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBA8455021.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4068..4472
                     /locus_tag="J1138_003260"
     CDS             4068..4472
                     /locus_tag="J1138_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBA8455022.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEEQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4498..5211
                     /gene="msyB"
                     /locus_tag="J1138_003261"
     CDS             4498..5211
                     /gene="msyB"
                     /locus_tag="J1138_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBA8455023.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5360..5926
                     /gene="satP"
                     /locus_tag="J1138_003262"
     CDS             5360..5926
                     /gene="satP"
                     /locus_tag="J1138_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBA8455024.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6131..6718)
                     /gene="mog"
                     /locus_tag="J1138_003263"
     CDS             complement(6131..6718)
                     /gene="mog"
                     /locus_tag="J1138_003263"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBA8455025.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6833..7786)
                     /gene="tal"
                     /locus_tag="J1138_003264"
     CDS             complement(6833..7786)
                     /gene="tal"
                     /locus_tag="J1138_003264"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBA8455026.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            8065..9495
                     /locus_tag="J1138_003265"
     CDS             8065..9495
                     /locus_tag="J1138_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBA8455027.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYAIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     CLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSFPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9565..10341
                     /gene="yaaA"
                     /locus_tag="J1138_003266"
     CDS             9565..10341
                     /gene="yaaA"
                     /locus_tag="J1138_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBA8455028.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10494..10793)
                     /locus_tag="J1138_003267"
     CDS             complement(10494..10793)
                     /locus_tag="J1138_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBA8455029.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPPRHHKKAHHDHHGGNGPGKHHR"
     gene            complement(11008..12294)
                     /gene="thrC"
                     /locus_tag="J1138_003268"
     CDS             complement(11008..12294)
                     /gene="thrC"
                     /locus_tag="J1138_003268"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBA8455030.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12295..13227)
                     /gene="thrB"
                     /locus_tag="J1138_003269"
     CDS             complement(12295..13227)
                     /gene="thrB"
                     /locus_tag="J1138_003269"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBA8455031.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(13229..15691)
                     /gene="thrA"
                     /locus_tag="J1138_003270"
     CDS             complement(13229..15691)
                     /gene="thrA"
                     /locus_tag="J1138_003270"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBA8455032.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15772..15837)
                     /gene="thrL"
                     /locus_tag="J1138_003271"
     CDS             complement(15772..15837)
                     /gene="thrL"
                     /locus_tag="J1138_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBA8455033.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16051..16737)
                     /locus_tag="J1138_003272"
     CDS             complement(16051..16737)
                     /locus_tag="J1138_003272"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBA8455034.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3967 a         4478 c         4143 g         4295 t
ORIGIN      
        1 tgatagagga aaacaccttt ttttgtgaat ccctggatat atttaagtta aataaattga
       61 tgtattgaaa tattattccg caaaaatgcg aggcgtcttc agaaaaagaa ttgataaaac
      121 agaagtgaat taaaagcgca gtataatagc ggcgggtgct tgaggctgtc tgtctcaggc
      181 attggctgaa cggcagatag agaaaagccc cgagtgatat tttaccatca acccgaggcc
      241 ccctatatgc agaacacatg tagagtgcct cttactgacc gaaaggtcaa ggagaagaga
      301 gcaatgaagc agcataaggc gatgattgtc gccctgatcg tcatctgtat caccgccgta
      361 gtggcggcgc tggtaacgag aaaagacctc tgtgaggttc acatccgaac tggccagacg
      421 gaggttgctg ttttcacggc ttacgaatcc gagtaagagc aacggcgggg agtgatcccc
      481 gccactttac aggtgctcgc atatcttcaa cgcaccctgg tttttaccct ggcctgccca
      541 cgggcaggct tttggggagg ttagcgagtc aggtcgtcaa aaaacttctt cacgccatca
      601 aagaagctct ttgaacgcgg gctgttgtgc tcgccggttg ggccaccaaa gctttcttgc
      661 agctcttgca gcagctgttt ctgcttctcg ttcaaaccta ccggtgtttc gacaacaacg
      721 cggcatagca aatcaccctg tgcgccaccg cggacagact tgacgccttt accgcgcata
      781 cggaacagct taccggtctg ggtttcgcca ggcactttca gtttgacgcg accatcaagg
      841 gtcggtactt cgatttcacc acccagcgcc gccatagcga agttgatcgg gacttcgcaa
      901 tacaggttgt tgccttcacg ctcgaaaatc gggtgctgtt taacctgaac ctgaacgtac
      961 agatcgcctg ccggtgcgcc gtgttcaccc gcttcacctt cgcccgcaag acggatgcgg
     1021 tctccagtgt ccacccctgc cgggatttta acggacagcg ttttgctgcg ctcaacacga
     1081 ccatgaccat gacatttgtt gcacggatct ttgatcagcg taccgcggcc ctgacagtgt
     1141 ggacaggtct gctgcacggc aaagaaaccc tggcgcatct gcacctggcc agaaccatga
     1201 caggtcggac aggtctgcgg ctgtgtacct ggttttgcac cgctaccgtg gcaaacgtca
     1261 cactcttcca gagtcggaat gcggatctct ttggtcacgc cacgtacagc ttcttcgagg
     1321 gtgagctcca tgttatagcg taaatcagca ccgcgcgccg cacgttgacg accacgtccg
     1381 ccgccaaaaa tatcgccgaa aacgtcacca aaaatatcgc tgaagtctgc gccgccgcca
     1441 aaaccgccgc cgcccatgcc gccttgctca aacgcagcat gaccatactg atcgtatgcc
     1501 gcacgttttt gcgagtcggt cagaacttca taagcttcct tgatctcttt aaatttcgcc
     1561 tcggcctctt tgtcaccctg gttacggtcc gggtggtatt tcatggccag gcgtttgtag
     1621 gcctttttga tttcacgctc ttccgctgtt ttggaaacgc ctaaaatctc gtaataatct
     1681 tgcttagcca tcttttttga tttgccccta gatgaatgca cgggcggaga ggaaattccc
     1741 cttcgcccgt gtcagtataa ttacccgttt atagggcgat tattttttgt ctttgacttc
     1801 ttcaaattca gcgtcgacaa catcgtcatc tttcgcgttg tttgcagaag catcagcacc
     1861 ggcagtctgc tgctgggcat gttgctgctg ggcgatttcc atcagtttct gggaaacctg
     1921 tgccagttcc tgcattttcg cttcgatagc ggctttgtct tcacctttca gagcagtttc
     1981 cagtgcagtc agcgcagact cgatagcagt tttgtcgtca gccggcagtt tgtcgcctgc
     2041 ttcttcaacc tgcttacggg tgctgtgcag cagatggtcg ccctggttgc gagtctgtac
     2101 cagctcttca aacttacggt cagcttcggc gttagcttct gcgtcgcgta ccattttctg
     2161 gatttcatct tcgttcagac cagaagaagc cttgatagtg atcttctgct ctttaccgct
     2221 gtttttgtct ttcgcggaaa cgtgcaggat accgtcagca tcgatatcga aggtaacttc
     2281 gatctgcggc atgccgcgcg gtgccgggtt gataccatcc aggttgaact gccccagaga
     2341 tttgttatca gccgcacgtt tacgttcacc ctgcagcaca tggatggtta ccgcagactg
     2401 gttgtcttca gcggtagaga acacctggct gtgcttggtc gggatagtgg tgtttttcgc
     2461 gatcagcgtg gtcatcacac cgcccatggt ttcgataccc agagacagcg gggtaacgtc
     2521 cagcagcagt acgtctttta cgtcaccagt cagaacacca ccctgaacag cagcaccgat
     2581 ggctacagct tcgtccgggt taacatcttt acgcggctct ttaccaaaga attcagcaac
     2641 tttcttctga accattggca tacgagtctg accaccaacg aggataacgt cgtcgatatc
     2701 agatacggac aggccagcgt cctgcagtgc aactttcagc ggctcaatgg aacggtttac
     2761 cagatcttca accaggcttt ccagtttcgc acgagtcact ttgatgttca tgtgtttcgg
     2821 accggtcgcg tctgcagtga tgtacggcag gttaacgtcg gtctgctgag cggaagacag
     2881 ttcgattttc gctttttctg ccgcttcttt caggcgctgc attgccagcg gatcgttgcg
     2941 caggtcaatg ccctgatctt tcttgaattc ttcaaccaga tagttgatca gacgactgtc
     3001 gaagtcttca ccacccaggt gggtatcacc gttggttgcc agaacttcga aggttttttc
     3061 gccgtcaact tcgtcgattt cgataatgga aatatcgaaa gtaccaccac ccaggtcata
     3121 aaccgcgata gtacggttgc cagtaccttt gtccagaccg taagccagcg cagctgcggt
     3181 cggttcgttg atgatacgtt ttacttccag accagcgata cggcctgcgt ctttggttgc
     3241 ctgacgctga gcatcgttaa agtatgccgg tacggtaata acagcttcag ttaccggttc
     3301 acccaggtaa tcttcagcgg ttttcttcat ttttttcagc acttcagcag agatctgcgg
     3361 cggtgccatt ttctggcctt taacttcgac ccatgcgtcg ccgttatcag cagcaataat
     3421 tttgaacggc atgatggaaa catcacgctg tacttcttcg tcctggaagc ggcggccaat
     3481 caggcgttta atcgcaaaca gggtgttttg cgggttcgtc actgcctgac gtttagccgg
     3541 ctgaccaacc agagtttcac catcctgggt ataggcaatg atagaaggcg tggtgcgatc
     3601 gccttcggcg ttctccagta cacgaggagt ggtgccatcc ataatcgcta cacaagagtt
     3661 ggtagtaccc aggtcgatac caattatttt acccatctaa acgtctccac tatatattcg
     3721 gtcatcatgt ggttgtgagt ctgtaatagg ggcgaaacgt ctggtttcaa ctgcccaatt
     3781 tcattttttt gcagactcac tcactgcggt tgactactaa atggggtcgt aacccacgtc
     3841 atcaaggggg agatgcaaaa attttttgtg cttttgcgca gaaatttcgc gatatggcag
     3901 gacaagacgt gaaactgaca acaattttat tcatataaag aatgaatcgc agccaaggca
     3961 ttcatcaaaa aattgtaata aaaagaaaat attacgtacc tgaaacttct ctttatcagc
     4021 agtaaactag tttcatcccc ttacccctcc ccaccaagag aatccttatg aaatccgtta
     4081 ttacgatttc cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg
     4141 accataaaat cctcggcgtc attgcaatgc cgcgaaacga aaccaacgat ctggcgctga
     4201 aacttcctgt ttgtcgcatt gttaaacgca tacaactctc agccgaccat ggcgatttgc
     4261 agttaagcgg tgcatcgatt tatttcaaag ccacccgaag cgccagtcag accctgaata
     4321 ttccttcaga aataaaagaa gagcaaacca ctgactggat caacattaac agcgataacg
     4381 acaacaaacg ctgcgtctca aaaatcacct tttccggtca tacagtgaac tcatcggata
     4441 tggccacgct gaaaattatc ggcgacgact aacgccagaa tatagaaacc acaaaaaatg
     4501 aatgttaatt acctgaatga ttcagatctg gattttctcc agcattgtag tgaggaacag
     4561 ttggcaaatt tcgcccggct gctcacccat aatgaaaaag gcaaaactcg cctctccagc
     4621 gtactgatgc gcaacgaact gtttaaatcg atggaaggac atcccgagcg acaccgccgc
     4681 aactggcagc tgattgccgg agaattacag cattttggtg gcgatagtat cgccaacaaa
     4741 ctgcgcggac acggtaaatt gtatcgcgcc attttgctcg atgtttcaaa gcgattgaag
     4801 ctgaaagccg acaaagagat gtctacgttt gaaattgagc aacagttact ggaacaattt
     4861 ctgcgtaata cctggaagaa aatggacgag gaacataagc aggagtttct gcacgcggtc
     4921 gatgccaggg tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga caaattattg
     4981 gcaaaaggtg tgtcgcattt gctttccagc caattgaccc gcattttacg cacccacgca
     5041 gcaatgagcg tgcttgggca tggtttgctg cgcggcgcgg gtctgggagg ccctgtgggc
     5101 gcagcattaa atggggttaa agcggtcagc ggcagtgcct atcgcgtgac aattccagcc
     5161 gtactgcaaa tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg ataccggaaa
     5221 ccggggaaaa ttttcatttt cccctcaaaa gatcgtagac actgccccac tggctgatta
     5281 ttatgccgcg ccctgaaaac actacagtta ttcagggaaa ttatttcacc attcattcga
     5341 tgatgatttt tgaggaatta tgggcaacac taagttggct aatccggcac cgctgggcct
     5401 gatgggcttc ggcatgacca ccattctgct taacctgcac aacgtgggtt attttgctct
     5461 ggacggtatt attcttgcca tgggcatttt ctacggcggc atcgcgcaaa tttttgccgg
     5521 tctgctggag tacaaaaaag gcaacacttt cggtttaacc gcattcacct cttacggttc
     5581 tttctggctg acgctggttg caattctgct gatgccgaaa ctggggctga ccgatgcgcc
     5641 aaatgcacag ttccttggtg tctacctggg tctgtggggc atatttacgc tgtttatgtt
     5701 cttcggcacg ctgaaaggcg cacgcgttct gcaattcgtt ttctttagcc tgaccgtgct
     5761 gtttgccctg ctggcgatcg gtaacattgc cggtaacgcc gcgatcatcc actttgccgg
     5821 ctggattggt cttatctgcg gtgccagtgc aatctatctg gcgatgggtg aagtgctgaa
     5881 cgagcagttt ggtcgcaccg ttctgccgat tggtgagtct cactaattat taagctcccc
     5941 ccgctcgaag ggataagatt gttaacaacg tgcgctttgt ttatgccgga tgcggcgtga
     6001 acgccttatc cggcctacaa aatcgtgcaa attcaatata ttgcaagaag atcgtaggcc
     6061 tgataagcgt agcgcatcag gcagttttgc gtttgtcatc agtctgatcc ccccgcttgg
     6121 ggggattttt ttattcgcta acttcgcgtc ttgcactctt cggtctgaat gctgcaacca
     6181 cttccggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg
     6241 caaaaatacc gtgcaccaca acgttaccct cagcgtcctt cacaccttcc agcgtctctt
     6301 taatagactt cggttgaccg ggtaagttaa ggatcagcgc ctgtttgcga atcaccccca
     6361 cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc atctgttcac
     6421 caaagcctgg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac
     6481 gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca tccaccagct
     6541 cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa
     6601 acggcgtggt tagcgccgat gtcagccatt cttccagcgc agggatgcct ttatcctgat
     6661 aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca
     6721 agatttttcc gtttaaacag tctcgttaaa cagaatgata cactgcgaag ggagtgacag
     6781 acaggtttca gaagaggtag cgtgaccgac ttcccggtca cgctaagtat gattacagca
     6841 gatcgccgat cattttttcc agtttttcct ggtcaacagc aaacttacgg ataccttccg
     6901 ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga
     6961 tacgcgccgg acgcgctttc acttcaccag tgtaagacag tttacgttcg atagcccctt
     7021 cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtcg cagcctgcca
     7081 gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccat
     7141 gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag
     7201 cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca acaaacggcg
     7261 agatcaggaa cacgcccgct tccgcacaag cacgagcctg agcgaaggag aacagcaggg
     7321 tcaggttaca gttgatgcct tctttttcca gctgttccgc agcacggata ccctgccagg
     7381 tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg tagagtttga
     7441 tcaggcgttt tgctttcgca attgacgctt cggtgtcata ggaaagacgc gcatcaactt
     7501 cagttgagat acggcccgga accagtttca ggatttccag accaatattt acggccagtt
     7561 tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga
     7621 cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga atgagagaag
     7681 ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg
     7741 ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct
     7801 ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc
     7861 agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg
     7921 cgttgatatc gcccgccatt tttatacaaa acctcatgta tgctacgcag aagttatcaa
     7981 gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg
     8041 cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt
     8101 tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc
     8161 ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa tagcattcat
     8221 ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc agcgcgcgtg
     8281 ggtagcggca acctggccgg cgttgcgctg gcaattaccg ccggtggacc tggagccgtc
     8341 ttctggatgt gggttgccgc atttatcggc atggcgacct cgtttgccga atgttccctc
     8401 gcacagcttt ataaagaacg tgatgccaat gggcagtttc gtggcggacc ggcatggtat
     8461 atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc
     8521 gcctacgcca taattttcag cggagttcag gcgaacgccg ttgcgcgagc cctgagtttt
     8581 tcttttgatt ttcccccgct ggtgacaggt attattctcg ctgtctttgc tatgctggcg
     8641 atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg
     8701 ataatctggg tactgacctg cctggtgatt tgcgtaatga atatcgggca acttccccac
     8761 gtcatttggt ctatttttga gagtgctttt ggctggcagg aagctgcagg cggcgcggcg
     8821 ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag
     8881 gcgggaatgg ggtcgacgcc aaacgcggca gcggcagctg cgtcctggcc tccgcatccg
     8941 gcagcgcaag gaattgtcca gatgattggc atttttatcg acaccctggt catctgcacg
     9001 gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc gctggaaggt
     9061 attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctggggtgc ggagtttgtt
     9121 accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa
     9181 aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgtct gcggatctgc
     9241 accttcgcaa cggtcatcgg cggcaccttg ctaagttttc cgctgatgtg gcaactggca
     9301 gatatcatta tggcctgcat ggctattacc aatttgaccg ccatattact gctctcgcct
     9361 gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt gcgcccggtg
     9421 tttgatccgt tgcgttatcc ggagatcggt cgccagcttt ctccagacgc gtgggatgat
     9481 gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc taaagtcggc
     9541 ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa aacgcttgat
     9601 taccaaagcc cgttgaccac cacgcgctat acgctgccgg agctgttaga caactcccag
     9661 cagctgatcc atgaagcgcg gaaactgacg cccccgcaga ttagctccct gatgcgcatc
     9721 agcgacaaac tggcgggtat caacgccgct cgctttcatg actggcagcc agatttcaca
     9781 ccggagaatg cccgccaggc gattctggcg tttaaaggtg atgtctacac cggcttgcag
     9841 gccgaaacct tcagcgaaga cgatttcgat tttgcccaac agcatttgcg aatgctttcc
     9901 ggcttgtatg gcgtactccg cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg
     9961 atccgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga tatcatcacc
    10021 aacaagctga acgaggcgct cgcagcacaa ggcgataatg tggtgattaa tctggcgtca
    10081 gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat caagccggtg
    10141 ttcctcgatg agaagaacgg caaatttaag atcatcagct tctacgctaa gaaagcacgc
    10201 ggtctgatga gtcgtttcat tattgaaaat cggctgacca aaccagagca actgacaggt
    10261 tttaatagcg aaggttactt ctttgatgaa gcttcctcca gcaatggcga actggtgttt
    10321 aaacgctacg agcagcgtta agtttgtagt caatgtcgga tgcggcgtga acaccttatc
    10381 cggcctacaa aagcatgcag attcaatata ttgcagaatt cacgtaggcc tgataagacg
    10441 cgccagcgtc gcatcaggcg ctgaatgccg gatagttacc cggcatttgt tatttagcgg
    10501 tgatgtttgc ctggaccatt accgccgtga tgatcatgat gagctttctt atggtgacgc
    10561 ggcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc ctcgccactc ataatgttgt
    10621 ttccaccagc cgtggtcgcg ccagtgaccg ccatcccagt aatagccacg attatcacga
    10681 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg cgctgccatg
    10741 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
    10801 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacggcagt
    10861 tgttattctc cgcaatccta atctctaagt taccgcattt attgagaatt tctccttttt
    10921 ccctgtcgtt tttctccata ttttcttcat aaaaaaagcc gggctgcata aaagcaaacc
    10981 cggcctgatt gagataatga atagatgtta ctgatgattc atcatcaatt tacgcaacgc
    11041 agcaaaatcg gcgggcaggt tatgcgaaag caagggtaaa tcagcacgtt ctgccagctc
    11101 ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa atttcgccgg
    11161 atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac gcagcgcacg
    11221 ataagctacg gcagcgtgcg gctccgaggt atagcccagt tcttttaact cacgcattgt
    11281 ctgttgcgtg gtttcatcat ccacggctgc ataccccagc tctttcagtt gccagatttt
    11341 gcggcggaac aactcttcca cacgcggcca gttgtttggc tggctaacat ccatcgcatt
    11401 ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcgtgcagga aacgtggtac
    11461 ggtatcgttc acgttggtcg cagcaataaa acgcttcacc ggcagaccga gtgacttcgc
    11521 cagcaggcct gccgttaaat caccgaagtt tccactcggt accgagacaa ccaactggtt
    11581 acgtgcttct tgcggcaact gtgcgacagc ctcaaagtaa taacaaatct gcgccagcaa
    11641 tcgactgatg ttgatggagt tagcagaatt cagccccagc gccactttca gttcttcatc
    11701 atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt
    11761 ttcgatattg ccgcccaatg tacagaacag tttttcttgc agtggactga ttttgcctcg
    11821 tggatagagg ataaccactt tcacattcgg taaaccgtag aaagcatgag ccactgccgc
    11881 tccagtatca ccggatgtcg cggtcagaat ggtcactggc ttatcgcccg caatatgggt
    11941 cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg gcccgtggaa
    12001 caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga aggcaaacgc
    12061 cgcacgtacg cgctcttcca ggatttcctg cgggatttca tcaccaataa acgccgagag
    12121 gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt cagtcaggct
    12181 gaattccggc aggtcgtgcg gaaaaaacag cccctgattt ttgcctaacc cctgggttac
    12241 ggcttgcgca aagctgacct gctcattgtg atctttcaga ttgtagagtt tcatttagtt
    12301 ttccagtact cgtgcgcccg ccgtatccag ctggcaaata tgaacaaaac cttcctgatt
    12361 ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg gcttgtcaca
    12421 cagagcgaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc cgatttccgc
    12481 aaccgcctgc cgcgcctgcc ggaagccagg caataaccgt tcacggtagg gttcagcgat
    12541 aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa
    12601 gcctgccaaa tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat
    12661 agccctggct tctgccgtcg agactttaat ccccggatac gccagcaccc acagccactc
    12721 atcaaaccca ggcacttgct ggctgatgat gtcgttttct tcgatcatca actgcatacc
    12781 accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagatac gcccttccag
    12841 ctcgcccatc aaagccagca aacgagtgtc attaagcggc ttgccacagt gttcattcat
    12901 cgccatcagc gccgcgacca ccgaacaggc gctggagcct aagcccgaac cgatcggcat
    12961 attcttttcc agagtcatcg ccactggaat ttgcttgcca agctcctggc aaaaacgctc
    13021 ccagcactga taaacgatat tttcccgtgg ctctgacggc agcttatcgg caaagcgtcc
    13081 gaggttgttg agactgaatg tctctgccgc ctcaaccgtg actacatctc cgagcaatgc
    13141 accatcaaca ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca tattggcact
    13201 ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag agggtacgta
    13261 gcagatcggc aaagacacca gcagctgtaa cgtcattgcc cgcaccatat ccgcgcagca
    13321 ccaacggcag cggctgataa tagtggctat agaaggccag ggcgttttca ccatttttca
    13381 ctttgaacag cggatcatta ccatccactt cggcaatctt cacgcgacag acgccatctt
    13441 catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc gccacacgcg
    13501 cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcgaca tcaccctcgg
    13561 cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc agttcacgtc
    13621 ccgtttcacg agcgagaatc aatagcttac gcgccacatc cataccagaa agatcatctc
    13681 gcgggtccgg ttcggtataa cccatttccc gcgccagtgt ggtcgcctcg gagaaactca
    13741 tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg ccggagaact
    13801 tcatcaattc atcaccagca ttgagcagat tttgcaggtt ctcgataacc ggtaatccag
    13861 ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttccgccgca taacgcaact
    13921 gatggtagta atccatcgac gaggtgttgg cctttttgtt cggcgtaaca acgtggaaac
    13981 cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaagta cagtcaacaa
    14041 tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg agattaaacg
    14101 gctctttggc ttgcgccagt tcttcctgcc agttttccag attaaggcca tgtacattgg
    14161 tgagcagtgc cttcgagtta gcaacaccgc agacacgtaa gtcgatatgt ttattcttca
    14221 accagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca ccgacgccaa
    14281 tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta acgcgcacgc
    14341 cagtggtcgc atcatcgtta ttgaccacga cagagattga gcgttcagaa gatccctgag
    14401 caatggcgac aatgttgata ttggcacggg ccagcgcggc aaagaatttc gccgagatcc
    14461 cacgtaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt tccgtcaccg
    14521 ccaacggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt
    14581 cagctcgcac acagtcgctt tgcggaacgc agaaactgat actgtattcg gaagatgatt
    14641 gcgtaatcag caccacggaa atacgggcgc gtgacatcgc tgcaaagacg cgcgccgcca
    14701 tgccaaccat ccctttcatc cccgggccgg aaacgctgaa cattgccatg ttattcagat
    14761 tggaaatgcc cttgaccggt aattcgtctt catcacggct ggcaccaatg agcgtacctg
    14821 gagcttgagg atttccggta tttttaatca ggcaagggat ctgaaactgg gcgatggggg
    14881 tgatggtgcg ggggtgaaga actttagcgc cgaagtaaga aagctccatc gcttcctgat
    14941 aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cggatcgcag gtataaacac
    15001 cgtcaacatc cgtccagatc tcgcaacaat cggcgcgtaa acaggccgcc agcaccgcag
    15061 cggagtagtc ggaaccgttg cgtcccagaa ccaccagctc gcctttttca ttaccggcag
    15121 tgaaaccagc catcagcacc atgtggtcag ccggaatgcg gcttgccgca atacggcggg
    15181 tggactcagc aatatcaacg gtagattcga ggtaatgccc cactgcaagc agtttttcga
    15241 ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc ataatggcga
    15301 tcgacatttt ctcgccacgg caaatcagcg cagcgttgat gctatccggg cactgcccca
    15361 acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctggtcg acgaaagttt
    15421 tcaattgtgc cagcggaaat cccggctggg cggcggcgag tcccgtcaga agttcggcaa
    15481 aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg gttttttcaa
    15541 tcatcgctac cagatggttg gtgattttgg cgggggcaga gaggacggtg gccacctgcc
    15601 cctgcctggc attgctttcc agaatatcgg caacccgcag aaaacgttct gcatttgcca
    15661 ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct ttggtcgaaa
    15721 aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc
    15781 accgttacct gtggtaatgg tgatggtggt ggtaatggtg gtgctaatgc gtttcatgga
    15841 tgttgtgtac tctgtaattt ttatctgtct gtgcgctatg cctatattgg ttaaagtatt
    15901 tagtgaccta agtcaataaa attttaattt actcacggca ggtaaccagt tcagaagctg
    15961 ctatcagaca ctcttttttt aatccacaca gagacatatt gcccgttgca gtcagaatga
    16021 aaagctgaaa atcacttact aaggcgtttt ttatttggtg atattttttt caatatcatg
    16081 cagcaaacgg tgcaacattg ccgtgtctcg ttgctctaaa agccccaggc gttgttgtaa
    16141 ccagtcgacc agttttatgt catctgccac tgccagagtc gtcagcaatg tcatggctcg
    16201 ttcgcgtaaa gcttgcagtt gatgttggtc tgccgttgtg tcacttttcg ccggttgttg
    16261 tattaatgtt gctaattgat agcaatagac catcactgcc tgccccagat tgagcgaagg
    16321 ataatccgcc accatcggca caccagtaag aacgtcagcc aacgctaact cttcgttagt
    16381 cagcccggaa tcttcgcgac caaacaccag agcggcatgg ctcatccatg aagatttttc
    16441 ctctaacagc ggcaccagtt ctactggcgt agcgtagtaa tgatatttcg cccgactgcg
    16501 cgcagtggtg gcaacagtga aatcgacatc gtgtaacgat tcagctagtg tcttgaaaac
    16561 tttaatatta tcaataatat caccagatcc atgtgcgacc caacgggcga ctggctccag
    16621 gtgtgcctga ctatcaacaa tccgcagttc gctaaaaccc atcgttttca ttgcccgcgc
    16681 cgctgcccca atattttctg ctctggcggg tgcaaccaga ataatcgtta tacgcatatt
    16741 gccactcttc ttgaccaaat aaccgcgaac agggtgatca ttgtcaactt attacgcgtt
    16801 gcgaatttac aaattcttaa cgtaagtcgc aggaaaagcc ctttgcttag cttaaaaaaa
    16861 gctaaactat ttcctgactg tac
//
LOCUS       DADTFG010000108        16732 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.109, whole genome shotgun sequence.
ACCESSION   DADTFG010000108 DADTFG010000000
VERSION     DADTFG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.109"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            346..1116
                     /locus_tag="J1138_003273"
     CDS             346..1116
                     /locus_tag="J1138_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBA8455035.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            1131..2072
                     /locus_tag="J1138_003274"
     CDS             1131..2072
                     /locus_tag="J1138_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBA8455036.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            2123..3409
                     /locus_tag="J1138_003275"
     CDS             2123..3409
                     /locus_tag="J1138_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA8455037.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            3406..3693
                     /gene="fixX"
                     /locus_tag="J1138_003276"
     CDS             3406..3693
                     /gene="fixX"
                     /locus_tag="J1138_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBA8455038.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            3752..5083
                     /locus_tag="J1138_003277"
     CDS             3752..5083
                     /locus_tag="J1138_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8455039.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            5191..5721
                     /gene="kefF"
                     /locus_tag="J1138_003278"
     CDS             5191..5721
                     /gene="kefF"
                     /locus_tag="J1138_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBA8455040.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            5714..7576
                     /gene="kefC"
                     /locus_tag="J1138_003279"
     CDS             5714..7576
                     /gene="kefC"
                     /locus_tag="J1138_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBA8455041.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            7767..8246
                     /gene="folA"
                     /locus_tag="J1138_003280"
     CDS             7767..8246
                     /gene="folA"
                     /locus_tag="J1138_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBA8455042.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            8332..8565
                     /locus_tag="J1138_003281"
     CDS             8332..8565
                     /locus_tag="J1138_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBA8455043.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            8568..8882
                     /locus_tag="J1138_003282"
     CDS             8568..8882
                     /locus_tag="J1138_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein CcdB"
                     /protein_id="HBA8455044.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(8879..9727)
                     /gene="apaH"
                     /locus_tag="J1138_003283"
     CDS             complement(8879..9727)
                     /gene="apaH"
                     /locus_tag="J1138_003283"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HBA8455045.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(9734..10111)
                     /gene="apaG"
                     /locus_tag="J1138_003284"
     CDS             complement(9734..10111)
                     /gene="apaG"
                     /locus_tag="J1138_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBA8455046.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            complement(10114..10935)
                     /gene="rsmA"
                     /locus_tag="J1138_003285"
     CDS             complement(10114..10935)
                     /gene="rsmA"
                     /locus_tag="J1138_003285"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBA8455047.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            complement(10932..11921)
                     /gene="pdxA"
                     /locus_tag="J1138_003286"
     CDS             complement(10932..11921)
                     /gene="pdxA"
                     /locus_tag="J1138_003286"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBA8455048.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            complement(11921..13207)
                     /gene="surA"
                     /locus_tag="J1138_003287"
     CDS             complement(11921..13207)
                     /gene="surA"
                     /locus_tag="J1138_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBA8455049.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            complement(13260..15614)
                     /gene="lptD"
                     /locus_tag="J1138_003288"
     CDS             complement(13260..15614)
                     /gene="lptD"
                     /locus_tag="J1138_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBA8455050.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWKHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFNNKYGSSTDGYATQKFSIGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15869..16684
                     /gene="djlA"
                     /locus_tag="J1138_003289"
     CDS             15869..16684
                     /gene="djlA"
                     /locus_tag="J1138_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBA8455051.1"
                     /translation="MQYWGKIIGVAVALIMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
BASE COUNT         3754 a         4167 c         4671 g         4140 t
ORIGIN      
        1 taagctgcat tctgtgattg ttatcacatt tttgtttcgg gtgaatagag ggcatttttt
       61 cgttaatttt gattattaat cagtttgtta tgttctgttg tgagtaaaaa aatagcatct
      121 gactttcaat attggtgatc cataaaacaa tattgaaaat ttcttttttc tacgccatgt
      181 tttcaatatt ggtgaggagc ttaacaatat tgaaagttgg atttatctgc gtgtgacatt
      241 ttcaatattg gtgattaaag ttttatttcc aaattaaagg gcgtgatatc tgtaattaac
      301 accaccgata tgaacgacgt ttccttcatg atttctggag atgcaatgaa gattattact
      361 tgctataagt gcgtgcctga tgaacaggat attgcggtca ataatgctga tggttcgtta
      421 gacttcagca aagccgatgc caaaataagc caatacgatc tcaacgctat tgaagcggct
      481 tgccagctaa agcaacaggc agcagaggcg caggtgacgg ccttaagtgt gggcggtaaa
      541 gccctgacca acgccaaagg gcgtaaagat gtgctatcgc gcggtccgga tgaactgatt
      601 gtggtgattg atgaccagtt cgagcaggca ctgccgcaac aaacggcgag cgtactggct
      661 gcagccgctc agaaagcagg ctttgatttg atcctctgtg gcgatggttc ttccgacctt
      721 tatgcccagc aggttggttt gctggtaggc gaaatcctta atattccggc agttaacggc
      781 gtcagcaaaa ttatctccct gactgcagat accctcaccg ttgagcgcga actggaagat
      841 gaaacagaaa ccttaagcat tccgctgcct gcggttgttg ctgtttccac tgatatcaac
      901 tccccacaaa ttccttcgat gaaagccatt ctcggcgcgg cgaaaaagcc agtccaggta
      961 tggtcggcgg cggatatcgg ttttaacgca gaggctgcct ggtcagaaca acaggttgcc
     1021 gcgccgaaac agcgcgagcg tcagcgcatc gtgattgaag gcgacggcga agaacagatc
     1081 gccgcatttg ctgaaaatct tcgcaaagtc atttaattac aggggatgct atgaacacgt
     1141 tttctcaagt ctgggtattc agcgataccc cttctcgtct gccggaactg atgaacggtg
     1201 cgcaggcttt agctaatcaa atcaacacgt tcgtcctcaa tgatgccgac ggcgcacagg
     1261 caatccagct cggcgctaat catgtctgga aattaagcgg caaaccggac gagcggatga
     1321 tcgaagatta cgccggtgtc atggctgaca ctattcgcca gcacggcgca gacggtctgg
     1381 tgctgttgcc aaacacccgt cgcggcaaat tactggcggc gaaactgggt taccgcctta
     1441 acgcggcggt gtctaacgat gccagcgctg ttagcgtgca ggacggtaaa gcgacagtga
     1501 aacacatggt ttacggcggt ctggcgattg gcgaagaacg cattgccacg ccgtatgcgg
     1561 ttctgaccat cagcagcggc acgttcgatg tggctcagcc agacgcgtca cgcactggcg
     1621 aaacgcacac cgtggagtgg caggctccgg ctgtggcgat tacccgcacg gcaacccagg
     1681 cgcgtcagag caacagcgtc gatctcgaca aagcccgcct ggtggtcagc gttggtcgcg
     1741 gtattggcag caaagagaac attgcgctgg cagaacagct ttgcaaggcg ataggtgcgg
     1801 agttggcctg ttctcgtccg gtggcggaaa acgaaaaatg gatggagcac gaacgctatg
     1861 tcggtatctc caacctgatg ctgaaacctg aactgtacct ggcggtgggg atctccgggc
     1921 agatccagca catggttggc gctaatgcgt cgcaaaccat tttcgccatc aacaaagata
     1981 aaaatgcgcc gatcttccag tacgcggatt acggcattgt tggcgacgcc gtgaagatcc
     2041 ttccggcact gaccgcagct ttagcgcgtt gatccactct ggcagggctg cattttggcc
     2101 ctgccgctga cagggagctc ttatgtccga agatatcttt gacgccatca tcgtcggtgc
     2161 agggcttgcc ggttcggttg ccgcactggt gctcgcccgc gaaggtgcgc aagtgttagt
     2221 tatcgagcga ggcaattccg ctggtgccaa gaacgtcacc ggcgggcgtc tctatgccca
     2281 cagtctggaa cacattattc ctggtttcgc tgactccgcc cccgtagaac gcctgatcac
     2341 ccatgaaaaa ctcgcgttta tgacggaaaa gtcagcgatg actatggact actgcaatgg
     2401 cgacgaaacc tcgccatccc agcgttctta ctccgttttg cgcagtaaat ttgatgcctg
     2461 gctgatggag caggctgaag aagcgggcgc gcagttaatt accgggatcc gcgtcgataa
     2521 cctcgtacag cgcgatggca aagtcgtcgg tgtagaagcc gatggcgatg tgattgaagc
     2581 gaaaacggtg atccttgctg atggggtgaa ttccatcctt gccgaaaagc tggggatggc
     2641 aaaacgcgtt aaaccgacgg atgtggcggt tggcgtaaag gaactgatcg agttaccgaa
     2701 gtcggttatc gaagaccgtt ttcagttgca gggtaatcag ggcgcggctt gcctgtttgc
     2761 gggatcaccc accgatggcc tgatgggcgg tggcttcctt tataccaatg aaaataccct
     2821 gtcgctgggg ctggtttgtg gtttgcatca tctgcatgac gcaaaaaaat cggtgccgca
     2881 aatgctggaa gatttcaaac aacatccggc cgttgcaccg ctgatcgcgg gcggcaagct
     2941 ggtggaatat tccgctcatg tagtgccgga agcaggtatc aacatgctgc cggagttggt
     3001 tggtgacggc gtattgattg ccggtgatgc tgccggaatg tgtatgaacc tcggttttac
     3061 cattcgcggt atggatctgg cgattgccgc cggggaagca gcagcaaaaa ctgtgctttc
     3121 agcgatgaaa agcgacgatt tcagtaagca aaaactcgcg gaatatcgtc agcatcttga
     3181 gagtggcccg ctgcgcgata tgcgcatgta ccagaaacta ccggcgttcc ttgataaccc
     3241 acgcatgttt agcggctacc cggagctggc ggtgggcgtg gcgcgtgacc tgttcaccat
     3301 tgacggcagt gcgccggaac tgatgcgcaa gaaaatcctc cgccacggca agaaagtggg
     3361 cttcatcaat ctgatcaagg atggcatgaa aggagtgacc gttttatgac ttctcccgtc
     3421 aatgtggacg tcaaactggg cgtcaataaa ttcaatgtcg atgaagagca tccgcacatt
     3481 gttgtgaagg ccgatgcaga taaacaggcc ctggagctgc tggtgaaagc gtgccccgca
     3541 ggtctgtaca agaagcagga tgacggcagt gtgcgcttcg attacgccgg atgtctggag
     3601 tgcggcacct gtcgcattct ggggctgggg tcggcgctgg aacagtggga atacccgcgc
     3661 ggcacctttg gtgtggagtt ccgttacggc tgatgttggt ttgatacgta atgccgtact
     3721 gactctcatt gcaaaaaaac aggaataacc catgcaaccg tccagaaact ttgatgatct
     3781 taaattctcc tctattcacc gccgcatttt gctgtgggga agcggtggtc cgtttctgga
     3841 tggttatgta ctggtaatga ttggcgtggc gctggagcaa ctgactccgg cgctgaaact
     3901 ggacgctgac tggattggct tgctgggcgc gggaacgctc gccgggctgt tcgttggcac
     3961 atcgctgttt ggctatatct ccgataaagt cggacggcgc aaaatgttcc tcattgatat
     4021 catcgccatc ggcgtgatat cggtggcgac gatgtttgtc tcatcccccg tcgaactgtt
     4081 ggtgatgcgg gtacttatcg gcattgttat cggtgcggat tatcccatcg ccacctcaat
     4141 gatcaccgag ttctccagta cccgtcagcg ggcgttttcc atcagcttta tcgccgcgat
     4201 gtggtatgtc ggcgcgacct gcgccgattt ggtcggctat tggctttatg atgtggaagg
     4261 cggctggcgc tggatgctgg gtagcgcagc gattccctgt ttgttgattt tgattggtcg
     4321 attcgaactg cctgaatctc cccgctggtt gttacgcaaa gggcgagtaa aagagtgcga
     4381 agagatgatg ataaaactgt ttggtgaacc ggtggctttc gaggaagagc agccgcagca
     4441 aacccgtttt cgcgatctgt ttaatcgccg ccattttcct tttgttctgt ttgttgccgc
     4501 catctggacc tgccaggtga tccccatgtt tgccatttac acctttggcc cgcaaatcgt
     4561 tggtttgttg ggattggggg ttggcaaaaa cgcggcactg gggaacgtgg tgattagcct
     4621 gttctttatg ctcggctgta ttccgccgat gctgtggcta aacactgccg gacggcgtcc
     4681 attgttgatt ggcagctttg ccatgatgac gctggcgctg gcggttttgg ggctgatccc
     4741 ggatatgggg atctggctgg tagtgatggc ctttgcggtg tatgcctttt tctctggcgg
     4801 gccgggtaat ttgcagtggc tctatcctaa tgaactcttc ccgacggata tccgcgcctc
     4861 tgccgtgggc gtgattatgt ccttaagccg tattggcact attgtttcga cctgggcact
     4921 gccgatcttt atcaataatt acggtatcag taacacgatg ctaatggggg cgggtatctc
     4981 gctgtttggc ttgttgattt ccgtagcgtt tgccccggag actcgaggga tgtcactggc
     5041 gcagaccagc aatatgacga tccgcgggca gagaatgggg taatttgttc agatttctct
     5101 cttttctgaa tcaatattat tgactataag ctgcgtgaat atatgactac actttgtggg
     5161 aaaacaaagg cgtaatcacg cgggctacct atgattctta taatttatgc gcatccatat
     5221 ccgcatcatt cccatgcgaa taaacggatg cttgaacagg caaggacgct ggaaggcgtc
     5281 gaaattcgct ctctttatca actctatcct gacttcaata tcgacattgc cgccgaacag
     5341 gaggcgctgt ctcgtgccga tctgatcgtc tggcagcacc caatgcagtg gtacagcatt
     5401 cctccgctcc tcaaactttg gatcgataaa gttttctcgc acggctgggc ttacggtcac
     5461 ggcggcacgg cgctgcacgg caaacatttg ctgtgggcgg tgacgaccgg cggcggggaa
     5521 agccattttg agattggtgc gcatccgggg tttgatgtgc tgtcgcagcc gctacaggcg
     5581 acggcaatct actgcgggct gaactggctg ccaccgtttg ccatgcactg cacctttatt
     5641 tgtgacgacg aaaccctcga agggcaggcg cggcactata agcaacgtct gctggaatgg
     5701 caggaggcac atcatggata gccatacgct tattcaggcg ctgatttatc tcggttcggc
     5761 ggcgctgatt gttcccattg cggtacgtct cggtctggga tcggttcttg gctacctgat
     5821 cgccggctgc attattggcc cgtgggggct gcgactggtg accgatgccg aatctattct
     5881 gcactttgca gagattggcg tggtgctgat gctgtttatt atcggcctcg aactcgatcc
     5941 acaaaggctg tggaagctgc gtgcggcagt gttcggcggt ggcgcattgc agatggtgat
     6001 ttgcggcggc ctgctggggc tgttttgtat gttacttggg ctgcgctggc aggtcgcaga
     6061 attgatcggc atgacgctgg cgctctcatc aacggcgatt gccatgcagg cgatgaatga
     6121 acgcaatctg atggtgacgc aaatgggccg cagtgccttt gcggtgctgc tgttccagga
     6181 tatcgcggcg atcccgctgg tggcgatgat cccgctactg gcagcgagca gtgcctcgac
     6241 gacgatgggc gcatttgttc tctcggcgtt aaaagtggcg ggcgcgctgg tgctggtggt
     6301 attgctgggg cgctatgtca cgcgtccggc actgcgtttt gttgcccgct ctggcttgcg
     6361 ggaagtgttt agtgccgtgg cgttattcct cgtgtttggc tttggtttgc tgctggaaga
     6421 ggtcggcttg tcgatggcga tgggcgcgtt tctggcgggc gtactgctgg caagctcgga
     6481 ataccgtcat gcgctggaaa gcgatatcga accatttaaa ggtttgctgc tggggctgtt
     6541 tttcatcggc gttggcatgt cgatagactt tggcacgctg cttgaaaatc cgttgcgcat
     6601 tgtcattttg ctgctcggtt tcctcatcat caaaatcgcc atgctgtggc tgattgcccg
     6661 gccgttgcag gtgccaaata aacagcgtcg ctggtttgcg gtgctattag ggcagggtag
     6721 tgagtttgcc tttgtagtat ttggtgcggc gcagatggcg aatgtgctgg agccagagtg
     6781 ggcgaaatcg ctgaccctgg cggtggcact gtcgatggcg gcaacgccga ttctgctggt
     6841 gatcctcaat cgccttgagc aatcttctac tgaagaggcg cgtgaagccg atgagatcga
     6901 cgaagaacag ccgcgggtaa tcattgccgg attcggtcgt tttgggcaga taaccggacg
     6961 tttactgctc tccagcgggg tgaaaatggt ggtgctcgat cacgacccgg accatatcga
     7021 aacgctgcgt aaattcggta tgaaagtgtt ttatggcgat gccacgcgga tggatttact
     7081 ggaatctgcc ggagcggcga aagcggaagt gctgattaac gccatcgacg atccgcaaac
     7141 caacctgcaa ctgacggaga tggtgaaaga acatttccca catttgcaga ttattgcccg
     7201 cgcccgcgat gtcgaccact acattcgttt gcgtcaggca ggcgttgaaa agccggagcg
     7261 tgaaaccttc gaaggtgcgc tgaaaaccgg gcgtctggca ctggaaagtt taggtctggg
     7321 gccgtatgaa gcgcgagaac gcgccgatgt gttccgccgc tttaatattc agatggtgga
     7381 agagatggca atggttgaga acgacaccaa agcccgtgcg gcggtctata aacgcaccag
     7441 cgcgatgtta agtgagatca ttaccgaaga ccgtgaacat ctgtcattaa ttcaacgaca
     7501 tggctggcag ggaaccgaag aagggaaaca tactggcaat atggcggatg aaccggaaac
     7561 gaagccctca tcctaataaa gagtgacgta aatcacgctt tacagctaac tgtttgtttt
     7621 tgtttcattg taatgcggcg agtccaggga gagagcgtgg actcgccagc agaatataaa
     7681 attttcctca acatcatcct cgcaccagtc gacgacggtt tacgctttac gtatagtggc
     7741 gacaattttt ttatcgggaa atctcaatga tcagtctgat tgcggcgtta gcggtagatc
     7801 gcgttatcgg catggaaaac gccatgccgt ggaacctgcc tgccgatctc gcctggttta
     7861 aacgcaacac tttaaataaa cccgtgatta tgggccgcca tacctgggaa tccatcggtc
     7921 gtccgttgcc aggacgcaaa aatattatcc tcagcagtca accgggtacg gacgatcgcg
     7981 taacgtgggt gaagtcggtg gatgaagcca ttgcggcgtg tggtgacgta ccagaaatca
     8041 tggtgattgg cggcggtcgc gtttatgagc agttcctgcc gaaagcgcaa aaactgtatc
     8101 tgacgcatat cgacgcggaa gtggaaggcg acacccattt cccggattac gagccggatg
     8161 actgggaatc ggtattcagt gaattccacg acgccgatgc gcagaactct catagctatt
     8221 gctttgagat tctggagcgg cggtaatttt gtatattaga attccggcat cactgatacg
     8281 tgcagatatc cgtgtatact agacgtataa attgtacagg agcacgagat catgactgca
     8341 aaacgtacca cacaaagtgt gaccgtcacc gtcgaccgtg agttagtcaa tcgcgctcgt
     8401 gatgcaggct taaatatgag cgccaccctt acggttgcgc tcaatgctga acttaaaaaa
     8461 catgcagcaa cacgttggcg tgaagagaac gcagaagcta tcgctgcgtt aaatcaattg
     8521 gctgatgaaa ccggatgttt ctctgatgag taccggagct tctagcgatg caatttacgg
     8581 tataccgcag tcgcggcagg aacgccgcgt ttccctttgt tattgatgtt accagcgaca
     8641 ttattggtga gattaatcgc cgtatcgtta ttccattaac acctattgaa cgatttagcc
     8701 gtattcgccc accagaacgg cttaacccaa tccttttact ggtagacggc aaagaatatg
     8761 tgctcatgac gcatgagact gcaactgttc cagttaacgc gctgggaacg aaattttgcg
     8821 atgccagcgc gcaccgaacg ttgattaaag gagcattaga ttttatgctc gacgggattt
     8881 aagacgctac cgcctctccc tcacccatat ctttatgccg gttcgacggc tggacaaaat
     8941 actgtttatc ttcccagcgc aggcaggtta atgtaccacc ccagcagcag ccggtatcca
     9001 gcgcgtaaat accttccggc gtacctttgc cttccagcga tgcccagtga ccaaaggcga
     9061 tgttgtactc ttcagcgacg gggccaggaa tcgcaaacca cggtttcagt ggggcagggg
     9121 cctcttccgg cgattctttg ctgtacatat ccagttgacc gttcgggaag caaaaacgca
     9181 tacgggtaaa ggcgttggtg ataaaacgca gtcttcccag cccccgcaat tccggtgacc
     9241 agttatttgg catatcgccg tacatggcat caaggaagaa gggataggag tcactcgaca
     9301 gcaccgcttc tacatcgcgc gcgcactctt tggcggtctg cagatcccac tgcggcgtga
     9361 tcccggcgtg ggccatcacc aactttttct cttcgtcgat ttgcagcaaa ggctggcgac
     9421 gcagccagtt aagcagctcg tcggcatccg gcgcttccag cagcggtgtc aggcgatctt
     9481 tcggtttatt gcggctgatc ccggcaaata ccgccagcag atgcagatcg tgattaccca
     9541 gcaccagacg tacgctgtcg cctaaggatt tcacatagcg cagaacatcc agcgaacccg
     9601 ggccgcgcgc gaccagatcg cccgtcagcc agagggtatc tttcccaggg gtaaattcta
     9661 ctttatgcag caatgcgatc agttcatcgt aacaaccatg aacgtcgcca ataaggtatg
     9721 tcgccatatt cttttaatga atgagtgtgg gaacggcgag tcggaatacg ggaatgtcga
     9781 tgctgaaagg gacgccattt tcatcgatca tttcgtagtg accctgcatg gtgcccagcg
     9841 gggtttcaat gattgcaccg ctggtgtact ggtactcttc gccaggcgcg ataagtggct
     9901 ggacgccaac cactccttcg ccctggactt cggtttcacg gccattgcca ttggtgatca
     9961 gccagtaacg ccccaacaac tgcactggcg ctcgccccag attgcgtatg gttacggtat
    10021 aagcaaaaac gtaacgttca ttatcaggtg aagattgggc ctcaatgtag acgctttgaa
    10081 cctgaataca cactcggggc gaattgatca tcgttaactc tcctgcaaag gcgcgttctc
    10141 cgccagatag ttcgccatct ggcaatattg cgcgacagag atattttccg ctcgcatcgc
    10201 cgggtcgatc cccattcccg ttaacacctc gacgctaaac aggttgccga ggctgttacg
    10261 aatggtttta cgacgctggt taaaggcttc agtggtgatg cggctcaaca cgcgaacatc
    10321 tttaaccggg tgaggcatcg ttgcatgagg aaccaggcgc acgacggcgg aatccacttt
    10381 gggtggtggt gtaaaggctg acggcggtac ttccagcacc gggatcacgt tgcaatagta
    10441 ttgcgccatg acgcttaatc gaccatacgc cttgctgttc ggtcctgcaa ccagacgatt
    10501 caccacttct ttttgcaaca taaagtgcat gtcggcaatg gcatcagtat agctaaacag
    10561 atggaacatc aacggcgtgg agatgttata aggcaggttg ccgaaaacac gcagcggctg
    10621 gcccattttc gcggccagtt cgccaaagtt aaaggtcatc gcatcctgct gataaatcgt
    10681 cagtttcggg cctaagaatg gatgcgtttg cagacgtgcc gccagatcgc ggtcaagttc
    10741 gatgaccgtc agctggtcca gacgttcgcc gaccggttcg gtcaatgccg ccagaccggg
    10801 gccgatttcg accatcgcct ggcccttctg cgggttaatg gcagaaacaa tgctgtcgat
    10861 cacgaactga tcgttgagaa agttttgccc gaagcgttta cgggctaagt ggccctggtg
    10921 gactcgatta ttcattgggt gttaacaatc attttgatgg cgagattaag cgccgtaata
    10981 aaactgccga catcggcttt accacgtccc gccagttcaa gcgcggtgcc gtggtccact
    11041 gatgtgcgaa taaagggcag gcccagcgta atgttcacac cgcgcccgaa gccctggtat
    11101 tttagcacgg gaagaccctg atcgtggtac atcgccagca cggcgtcggc gttatcgaga
    11161 tatttcggct gaaacagggt atcggcaggc agcggcccgt tgagtttcat cccctgttcc
    11221 cgcagctcgt tgagtaccgg aataatggtg tctatctctt ccgtacccat atgaccgcct
    11281 tcgcccgcgt gcggattcag cccgcagacc agaatgcgcg gttcggcaat accaaatttg
    11341 gtgcgcaaat cgtgatgcaa aatagcaatc acttcgtgca aaagggcagg ggtgatagca
    11401 tctgcgatat cgcgcagcgg taaatgcgtc gttgccagcg ccacgcgaag ttcttcggtt
    11461 gccagcatca tcaccacttt tttcgcctgc gaacgctctt cgaaaaactc ggtatgaccg
    11521 gtaaacggaa tgcctgcgtc gttaatgacg cctttatgca cggggcctgt gatcagcgca
    11581 gcaaattcac cgttcagaca gccatcacac gctcgcgcca gcgtttccac cacatagtgc
    11641 ccattttcaa ctgctaactg ccccacagtg acagattcac gtagcgcgac aggaagtagc
    11701 gttaatgtgc ccgcagtttg cggttgtgca ggggagttgg gggaataagg gcggagggtg
    11761 agcggcaaac cgagcatcgc tgcccggtcg gtaaggagag tggcatcggc acaaacaacc
    11821 agttcgaccg gccactcacg ctgtgcaagc tggacaatta agtccgggcc aatcccggcg
    11881 ggctcgccgg gagtgatcac aacacgttgg gttttaacca ttagttgctc aggattttaa
    11941 cgtaggcgct ggcacgttgt tcctgcatcc agcttgctgc ttcttccgag aacttacggt
    12001 tcatcagcat gcggtatgca cgatctttct gtgccgcgtc ggttttatcg acattacggg
    12061 tatccagcag ttcgattaaa tgccagccga atgaagagtg aaccggtgca ctcatttgac
    12121 ctttgttcag gcgggtcagg gcgtcacgga aggccggatc gaaaatatct ggtgtagccc
    12181 agccgagatc accgccctgg ttagcagagc ctggatcctg agagaactct ttcgctgcgg
    12241 cagcaaaagt cgttttacca ctcttgatat cggcagcaat ctgttccagt ttcacgcggg
    12301 cctgttcgtc agtcatgatc ggcgacggtt tcagcagaat atggcgagca tggacttcgg
    12361 tcaccgagat atttttgctt tcgccgcgca ggtcgttaac tttcagaata tggaagccaa
    12421 cgccggaacg aatcgggcca acaatgtcgc ctttcttcgc ggtgcttaat gcctgggcga
    12481 agatccccgg caactcctga atacggcccc agcccatctg tccgccgttc agcgcctgct
    12541 ggtcggcaga atgagcaatc gccagcttac cgaaatcagc gccgttacgc gcctgatcga
    12601 caatcgcgcg cgcctggctt tccgcttcgt tcacctgatc agaggtcggg ttttccggca
    12661 gtgggatcag gatgtggctc aggttcagct cagtgctggc gtcgttttgg ttacccactt
    12721 gctgcgccag ggattcgact tcctgcggca ggatggtgat gcgacgacgc acctcgttgt
    12781 tacgcacttc agagataatc atctctttgc ggatctggtt acgataggtg ttgtagttca
    12841 gaccatcgta agccagacgg ctgcgcatct gatccagcgt catgttgttc tgtttcgcga
    12901 tgttagcaat cgcctgatcc agctgctcat cggagatttt cactcccatt ttctgcccca
    12961 tctgcaggat gatttgatcc atgatcaaac gttccatgat ttggtggcgc agcgtcgcgt
    13021 catcaggaag ttgctgcctt gcctgagcag cgttcagttt taccgactgc attaatccat
    13081 caacgtcgct ttccagcacg acgccgttat tgacgacggc tgcgacttta tcgactacct
    13141 ggggggcagc gaaactggta ttcgcgatca tggcgatacc gagaagcagc gttttccagt
    13201 tcttcatact ttttccattt caattaaccg cactgcggat tacgtggtaa atcaacaaat
    13261 cacaaagtgt tttgatacgg cagaatgttc gaacgcagca tctcttgcgt acccagaccg
    13321 tagttggagc tcaggccgcg aagttcgatg ttaaagccga ttgcgttgtc atataccgca
    13381 tgttgtttat cgttatccca accgttcagc ttccgctcgt aaccgacgcg aatcgcatag
    13441 cagcaggagc tgtattgcac acctaacata gagtcggctt gcttgttcgc attggtgtcg
    13501 tagtagtagg ccccaacaat ggaccaacga tcggcaattg gccagctggc gacggcacct
    13561 acctgcgaaa taccattctt atattgctca gcagtggaat agtacttagg cagcgttgcc
    13621 tgaatatatt ccgggctggc gtaacggtaa ttcagctgta ccagacggtc ttcatcccga
    13681 cggtattcaa tgctggagtt actggtcgct acgttatcca gacgtgtatc gtactgaatc
    13741 ccgccacgca atccccaacg atcagagatg cgccagtaag tatcgcctgc ccataccagt
    13801 gagcccgttt tgtcgtcatt ctcccatgtt atgttgtcat cgccagtgcg agactccgtg
    13861 aaatagtaga tttgaccaac ggaaatatta aaacgttcaa cggcagcatc atcatatatg
    13921 cgagatgtga caccggtcgt cacctggtta gcggaggcaa tacggtcaag accgccgtaa
    13981 gtccggtccc ggaacaggcc ggagtagtca gattgcagca gcgacgagtc gtagttatag
    14041 attttgctct gatcgcgata tggcacatac agatactgcg cgcgtggttc cagtgtctgg
    14101 gtatagcccg gcgccagcat ttccatatca cgctcgaaga ccattctacc gtctaccttg
    14161 aactgcggca ttacacggtt agcggactct gccagcctgg tcgtgtttct ggagttatac
    14221 cagtcaagat tggtttgctg ataatgggtt gccagcaact tcgcttcagt attgatgctg
    14281 ccccagttat tagagagcgg cagattgatg gtcggttcca ggtgaacacg agtcgcctct
    14341 ggcatgtcat ctctggtgtt aacaaagtgc actgcctggc cgtaaatacg cgtatcaaac
    14401 ggaccaacat cattctggta gtagttaacg tctaactgcg gctctgccga gtagctactg
    14461 gtgttctgct cgctgaagac ctggaactgc ttggttgaaa cggtggcatt gaagttttgc
    14521 accgcataac caatgctgaa tttttgcgtt gcgtagccgt cggtactgga accgtatttg
    14581 ttattgaagt cattgaagta gctaggatcg ctgactttgg tgtagtcgac gttgaaacgc
    14641 cacacctgat ccatgacccc ggagtgtttc cagtagaaca accaacgacg tgaactgtca
    14701 tcgttcgggt gttcatcttt atagacttta tctgaaggca gatagtccag ttccatcaaa
    14761 ccagcgcccg cctgggagag gtagcggaat tcgttctccc acatgatgtt gccacgacga
    14821 tgcatataat gcggcgtgat ggtggcatcc atatttggcg ctatgttcca gtaatatggc
    14881 aggtagaact caaagtagtt ggtggtggtg tacttggcgt tcgggatcaa gaaaccagag
    14941 cgacgtttgt cacccaccgg caactgcaaa tacgggctat aaaagatcgg taccggaccc
    15001 accttaaagc gggcgttcca gatctccgca acttgttctt cgcggtcatg aataatttcg
    15061 ctacctacca cgctccaggt gtcagaaccc ggcagacagg aggtaaagct accgttatcc
    15121 agaatggtat agcggttttc gccacgttgt ttcatcaggt ccgctttacc gcgaccctgg
    15181 cgacccacca tctggtaatc accctcccag acgttggtat ctttggtgtt cagattcgcc
    15241 cagcctttcg gccctttgag gatcacctgg ttatcgtcgt aatggacatt accgagcgca
    15301 tcaacggtac gtaccggctc cggttgtcct ggtgcctctt tttgatggag ctgcacttcg
    15361 tcggcctgca gacggctgtt accctgcatg atatccacgc tgccagtaaa cacggcgtca
    15421 tccgggtagt cccctttcgc gtggtcagca ttgatagtca cgggtaagtc attggtatcg
    15481 ccctgtacca gaggacggtc atagcttggc acgcccaaca tgcactgtga ggcgaggtcg
    15541 gctgccagtc cctgttgact ataaagggcg gtggcaatca tggtggccag gagagtgggg
    15601 atacgttttt tcatacgttg attttattgt tccatcatcg gtaacgttgc gcgtgacaaa
    15661 cggtcagaga ctaacgtact cgtcatctct acgctagtgt taatcctgtc cgaatagcgt
    15721 ctgtggtgtt aggcacggca ttgaatgaca ggtatgataa tgcaaattat aggcgatgtc
    15781 tcacaataga ccgcagccag aaatgggtaa aagcgccttt atattgtggg agatagccct
    15841 gatatccgtg tgtcgatttg gggaatatat gcagtattgg ggaaaaatca ttggcgtggc
    15901 cgtggcctta ataatgggcg gcggcttttg gggcgtagta ttgggcctgt taattggcca
    15961 tatgtttgat aaagcccgta gccgtaaaat ggcgtggttc gccaaccagc gtgagcgtca
    16021 ggcgctgttt tttgccacca cttttgaagt gatggggcat ttaaccaaat ccaaaggtcg
    16081 cgtcacggaa gctgatattc atatcgccag ccagttgatg gaccgaatga accttcatgg
    16141 cgcttcccgt actgcggcgc aaaatgcgtt ccgggtggga aaatcagaca attacccgct
    16201 gcgcgaaaag atgcgccagt ttcgcagtgt ctgctttggt cgttttgact taattcgtat
    16261 gtttctggag atccagattc aggcggcgtt tgctgatggt tcactgcacc cgaatgaacg
    16321 ggcggtgctg tatgtcattg ctgaagaatt agggatctcc cgcgctcagt ttgaccagtt
    16381 tttgcgcatg atgcagggcg gtgcacagtt tggcggcggt tatcagcagc aatctggcgg
    16441 tggtaactgg cagcaagcgc agcgtggccc gacgctggaa gatgcctgta atgtgctggg
    16501 cgtgaagccg acggatgatg cgaccaccat caaacgtgcc taccgtaagc tgatgagtga
    16561 acaccatccc gataagttgg tggcgaaagg tttgccgcca gagatgatgg agatggcgaa
    16621 gcagaaagcg caggaaattc agcaggccta tgagctgata aagcagcaga aagggtttaa
    16681 ataagtttca acgtgtaggc ctgataagac gcggtaggcc ggataagacg cg
//
LOCUS       DADTFG010000109        16704 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.110, whole genome shotgun sequence.
ACCESSION   DADTFG010000109 DADTFG010000000
VERSION     DADTFG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.110"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(266..802)
                     /locus_tag="J1138_003290"
     CDS             complement(266..802)
                     /locus_tag="J1138_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBA8455052.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSTAPDIVAARKSGTTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLNWLKALDEKYALRVIQIDVS
                     AGEKPGMVNVQRLEFGRG"
     gene            complement(804..1982)
                     /gene="gspL"
                     /locus_tag="J1138_003291"
     CDS             complement(804..1982)
                     /gene="gspL"
                     /locus_tag="J1138_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBA8455053.1"
                     /translation="MSSMLEIFFPLCAADPIRWQRHTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDIPLSLTVVGIAPEELSAWAGADAERLTVTALPAIITYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALVVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALVADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1979..2956)
                     /gene="gspK"
                     /locus_tag="J1138_003292"
     CDS             complement(1979..2956)
                     /gene="gspK"
                     /locus_tag="J1138_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBA8455054.1"
                     /translation="MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYATNQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2953..3558)
                     /gene="gspJ"
                     /locus_tag="J1138_003293"
     CDS             complement(2953..3558)
                     /gene="gspJ"
                     /locus_tag="J1138_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBA8455055.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQASWSSGQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3555..3926)
                     /gene="gspI"
                     /locus_tag="J1138_003294"
     CDS             complement(3555..3926)
                     /gene="gspI"
                     /locus_tag="J1138_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBA8455056.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSLH
                     DDFSPVIQSRRAWFSAVGGQQ"
     gene            complement(3923..4486)
                     /gene="gspH"
                     /locus_tag="J1138_003295"
     CDS             complement(3923..4486)
                     /gene="gspH"
                     /locus_tag="J1138_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBA8455057.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALEMQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLVHDGALSLNQCDEGMP"
     gene            complement(4490..4945)
                     /gene="gspG"
                     /locus_tag="J1138_003296"
     CDS             complement(4490..4945)
                     /gene="gspG"
                     /locus_tag="J1138_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBA8455058.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4962..6185)
                     /gene="gspF"
                     /locus_tag="J1138_003297"
     CDS             complement(4962..6185)
                     /gene="gspF"
                     /locus_tag="J1138_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBA8455059.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEA
                     RMNASAGGLLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALADLRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6185..7678)
                     /gene="gspE"
                     /locus_tag="J1138_003298"
     CDS             complement(6185..7678)
                     /gene="gspE"
                     /locus_tag="J1138_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBA8455060.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSHELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NQSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCS
                     DCRQAAPATDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7678..9738)
                     /gene="gspD"
                     /locus_tag="J1138_003299"
     CDS             complement(7678..9738)
                     /gene="gspD"
                     /locus_tag="J1138_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBA8455061.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRAEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLM
                     QFANGTQIPIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9768..10727)
                     /gene="gspC"
                     /locus_tag="J1138_003300"
     CDS             complement(9768..10727)
                     /gene="gspC"
                     /locus_tag="J1138_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBA8455062.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLSQRQSLNTDKEHLRKIARAM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAASDEAKQAVAEPVVSAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(10745..11155)
                     /gene="gspS2"
                     /locus_tag="J1138_003301"
     CDS             complement(10745..11155)
                     /gene="gspS2"
                     /locus_tag="J1138_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HBA8455063.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11221..12030)
                     /locus_tag="J1138_003302"
     CDS             complement(11221..12030)
                     /locus_tag="J1138_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBA8455064.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12186..>16704)
                     /gene="sslE"
                     /locus_tag="J1138_003303"
     CDS             complement(12186..>16704)
                     /gene="sslE"
                     /locus_tag="J1138_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HBA8455065.1"
                     /translation="LSATLLAGCDGGGSGSSSDTPSVDSGSGTLPEVKPDPTPTPEPT
                     PEPTPDPEPTPDPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSDGFTF
                     TPGNTVSCVVGSTTIATFNTQSEAARSLRAVDKVSFSLEDAQELANSENKKTNAISLV
                     TSSDSCPADAEQLCLTFSSVVDRARFEKLYKQIDLATDNFSKLVNEEVENNAATDKAP
                     STHTSTVVPVTTEGTKPDLNASFVSANAEQFYQYQPTEIILSEGQLVDSLGNGVAGVD
                     YYTNSGRGVTDENGKFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANID
                     QLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGDQNVVLPNE
                     FIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYQS
                     VSKFHVFHDSTNFYGSTGNARGQAVVNISNSAFPILMARNDKNYWLAFGEKRAWDKNE
                     LAYITEAPSIVQPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNGYSW
                     GGGVNSKGECTLSGDSDDMKHFMQNVLRYLSNDIWQPNTKSIMTVGTNLENVYFKKAG
                     QVLGNSAPFAFHEDFTGITVKQLTSYGDLNPEEIPLLILNGFEYVTQWSGDPYAVPLR
                     ADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASSFVRLLDAAGLSMALN
                     KSVVNNDPQGYPDRVRQRRATGIWVYERYPAADGAQPPYTIDPNTGEVTWKYQQDNKP
                     DDKPKLEVASWQEEVEGKQVTRYAFIDEAEYTTEESLEAAKAKIFEKFPGLQECKDST
                     YHYEINCLERRPGTDVPVTGGMYVPRYTQLNLDADTAKAMVQAADLGTNIQRLYQHEL
                     YFRTKGSKGERLNSVDLERLYQNMSVWLWNDTKYRYEEGKEDELGFKTFTEFLNCYAN
                     DAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWD
                     LNIKVDVEKYPGSVSAKGESVTENISLYSNPTKWFAGNMQSTGLWAPAQQDVTIKSSA
                     SVPVTVTVALADDLTGREKHEVALNRPPRVTKTYTLEANGEVTFKVPYGGLIYIKGDS
                     KDDVSANFTFTGVVKAPFYKDGEWKNDLDSPAPLGELESASFVYTTPKKNLEASNFTG
                     GVAEFAKDLDTFASSMNDFYGRNETSGNHRMFTYEALTGHKHRFANDVQISIGDAHSG
                     YPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLNVPGATEVANNVLALYMQDR
                     YLGKMNRVADDITVAPEYLDESNGQAWARGGAGDRLLMYAQLKEWAEKNFDITKWYPD
                     GKLPAFYSEREGMKGWNLFQLMHRKARGDDVGNSTFGGKNYCAESNGNAADTLMLCAS
                     WVAQTDLSEFFKKWNPGANAYQLPGAAEMSFEGGVSQSAYNTLASLKLPKPEQGPETI
                     NKVTEYSMPAE"
BASE COUNT         3687 a         4784 c         4221 g         4012 t
ORIGIN      
        1 caattgttac tcaatgattt attacaacta ttaataatca taatgtgtca aaacacttaa
       61 gcatctttac agtcttagaa aggtgtcgat taacgaaggc tacatggtga aaaacaggtt
      121 ccttcagtgt taccgaatat tccgtggtgg tctggaggga gataattctt gctacatcga
      181 aactcagccc ggtgagcggg tttgacagtt ccgcactcac cattgggcta agggttatca
      241 ggtggggtta aggaaatggc aatacctacc cccgcccaaa ttccagtcgc tgcacattca
      301 ccatcccagg cttctcacct gcgctgacat caatttgtat cacccgcagc gcatattttt
      361 catccagtgc ctttaaccag ttcagcaggt cattaaacac cacaggttct atccagactt
      421 gaatattctc cccacgctcg gcaatccgtt tgatgaccac cgagtgcgcg gaagcgctgt
      481 cgctgatgac ccgcgatacc tgtgctggcg ttgtcgtgcc ggattttcgc gccgcaacaa
      541 tatccggcgc agtgctcttc agtcgcgcgt tcatcgccac gagctgctgc aacatcgtct
      601 cctgttgttc tatccgttcg ctcaacggct gccagatgag aacgtaatat ccggcgctaa
      661 acaggaacac taccgctgcc agtaacatgc ctttttcacg cggagaacgc cccgccaggt
      721 gttgtgtcag ccagtgttcg ccacgactta actggcgttc acgccattgc tgaaaatagt
      781 gaataaattt atcgcgtaac atgttatttc ctccgcaacg ttacgccgcc ggaaaccgca
      841 tccccctctt tctgtaacgc gtcctgttgc acaacataat ctgccaccag tgcgctacgc
      901 agtttgtcga agctggcaaa gttcgcagcc cgcagttgga ggtgaagcgt ctggcgtttt
      961 tgatcaaagg tgaaaccacg catctcaatg tcggaaagtg atgcggattt cagggtactg
     1021 gcgatcgctg acaattctgc gagcagccgg gtatcgtcgg cctgtgggcg atattttttc
     1081 agcgccatcg tcacctgaga gcgtaaattc acaatccgct tctgctccgg gaatagcgtt
     1141 aagaactgtt tctccgcctg ggtgcggctt tgcgccacct gttcgctgac gctccataac
     1201 gtcacgcccc gttccactac cagcgcaacc agaatcagca atatcggcag aatcatcacc
     1261 cgccagcgcg cccactgttt tcggtagctg acacgaggct gccacggccc tgttagcagg
     1321 ttcccttccg gttcgccata agtgataatg gctggcagag ccgtaacggt caggcgttcg
     1381 gcgtccgcac cagcccatgc agacagttct tccggtgcaa tgccgaccac ggttagtgaa
     1441 agcggtatat cctgctcatt gagctgtgcg cggaacatga ccggagccag cgcccgcccg
     1501 gcgctccatc cccggcattc atcgatgcgg cagataaccc gttgcgcatc gccagccata
     1561 aacccacaag gaatagacat ccagtccggc gcgacggtag cgcgggtgat gccgtttgcc
     1621 tgcaaccact gcgcaatgtt gcgcatatgc tgctggtgaa tcacagctac ggttgccagt
     1681 tgctggtcga ttttcaacgg ggcgaaatgc agttcatcga tatcctggtt cagctcttct
     1741 tccagcaagg cgggcagaat cgtcggtatc tgcttgcggg gcacatcagg cagttcaacc
     1801 tgccagacgc tgatccattc gccgggaatg tagagtcgaa tcgcatcagt ttgcagccat
     1861 tgctggagac gttcgtcagc aacgtcaggc cagataccgt gctccacgtc gggtgtatgg
     1921 cgctgccaac ggatgggatc agcggcgcaa agcgggaaaa aaatctcaag catggaactc
     1981 actcactttc tcctgtctga tgccagagaa cagaaaagtg ttgtgggccc atgcggacaa
     2041 ttaacgaatt catcgtcagt tcaatctcat tcacggtgat atctgaacgc agccagaagt
     2101 aattgctgtc cacgctcagg acggttttta gctgtttttt agtacgctca tcgacgtctg
     2161 caagtagcgg ctgagcaaga aattgatcga catcttccca gcccttcgcc ggacgttgtt
     2221 gtaataacgc ccgcgcctga acagggctta accacgggtc aaacagcgcc tcaagaatca
     2281 cactttgcgt gacatctaat gtattgatgt tgatttgctg gcgggccatc ggcagcgcac
     2341 agaccaacgg tttcagtttt tgataaagcc cggcgtccat tccctgtacc acgcgcattt
     2401 cgctgatatc cgccagcggt tgattagtgg cgtagaacgg caccgagcgg gcgagatact
     2461 cgctgtcttc acgccccaga cgcgtctgca cgctgcggtc ttcgtcaata aactcccaca
     2521 ggctttcggc tatcagttcg gcccgataag caggtacatc aaggcgcgag atcagggcaa
     2581 tcagttgttg taccgcgagc ggacgcgacg ccgtcgtcgg ctgagcgagg gcattcaggt
     2641 taaagcaggc ctgtgcgtca cgcagagtga cggcgatttg cccctgcggc agtgggaaaa
     2701 aacgcgggcc agaagcccac ggttgtgcca gatgcacacg cttttcattt ttcaggctca
     2761 gactgagcgc gctcagcgcc aggctttctg cactggcgct gtaccacagc gcctgctggt
     2821 actcctgctg gctgcgcgtt cgcccaagtt gttgctgcat ccgcccggaa agcgtgatgg
     2881 tcaccagcat cataaccgcc agcaatacca gcaccacgac cagtgccatt ccgcgttttg
     2941 gtggtaaggt gatcatgata attgcggccc gcgtaacaac cagatgcgtt caatctcgcc
     3001 ccattgcggc gaatgcagag tcatgcgcac cgccacaggg atcgcctgcc ctgacgacca
     3061 actcgcctgc cagcgcgtgc cgtcgtagaa ctgcaaacgg agcgaatccg ccggaattaa
     3121 tttttgcgtt gttggcttca cgctgcccgc tgcatcggtc agcggccagg ctaaccgttc
     3181 aaggtaaccg tcatgaatgc ggtaaccgac agtgagcaga ttactgcgcg gcaggcgcat
     3241 caacggatta accacgccgc cacgcacaaa gcgcatccct tcactctcag acgccagcac
     3301 gccagcgccc gccagtaacg ctggttcacg ctggccctga tcgcctctta ccggacgcgg
     3361 catcatttgt gtcagatcgt ggttcaaaaa actcatcgtt tgctgcatga ggtttagttt
     3421 ttgatcgtgt ccggcgacgg cgctattcac gcgtgtaacg ccgtttgtca cctgctgcgc
     3481 catcagtgcc agtgaggcaa aaatggctat cgccaccagc atttccagta acgtgaaacc
     3541 agcgcgagcc cttctcactg ttggcctccc acggcgctaa accaggcgcg tcgtgactga
     3601 atcactggcg aaaagtcgtc atgaagactg acttcaatat ccaccgcctg gagcagcgcg
     3661 ttacctgtat tcagagggat cgttcgccag taccagcgac ttcctgccat atcactctcg
     3721 ccctggtgcc tgaccgctcg ttgttcacgg gtcatcaggt aaagcagcgc ggtctggttt
     3781 tcagctaccc aaccagccac cgttttttcc tcaagaacgt gctgattact cagcgcaccg
     3841 ctggcaatct gtaacaccgc catggcggcc aacgcaaaaa tcgccagcgc gagcattact
     3901 tccagcaagg taaatccacg cttcatggca tcccctcatc acattgattg agggataacg
     3961 cgccatcgtg caccagcttt accgcccaac acgcgttttg tgccgccgag tagaagcgca
     4021 gcgtaaacgg cgtggcgggt tcaaaaggcg aaaaacggat ctgcggcgtc ttctttttcg
     4081 cctctttttg cagctccagt tcaatatcgt gcagcgtcag gcgacgacgt tgcatctcca
     4141 gcgcatattc cttctgccag atatcactgc cgggttgtaa cagcatctgc acctgttttg
     4201 gcaccgtaac ctgtgccgat aagcgggtcg aggaaacggg caaccactgt ccgtgacgac
     4261 gctgcataaa ctggtaacct ggtgggtcga ttagcacacc gagtgttttc ccttcgatca
     4321 ctgccctgtc cttaaactgc gcaaagcgag tcagaaaatc ctgcgccgct tttttcgcgg
     4381 gcggttcaga atcggtcgca aaagtctgca ccacgcccgc actggcaagg ccgataagga
     4441 aaatcaccag catgatttcc agaagtgtga atccgcgttc aggcactgat tactgaaact
     4501 cctgcaaatt ccagttaccg atatctgcgc cagcgccctc cccgttttcc tgaccatctg
     4561 cccccagggt ataaacatca aacagccctt tttcacccgg gctgagatac tgataatcat
     4621 tgccccacgg atcctttggc agtcgtttga tgtatccacc ggtacggtag ttgcgggcgt
     4681 ccgccatatt ggctggctgc tggatcagcg cctccagtcc ctgttcggtg gtcggataac
     4741 gcccgttatc cagtcggtac atatccagcg cattctccag cgccacgata tcgctgatgg
     4801 ctttttgccg atcggctttc tctttgttgc ccagcaggtt aggcactacc agacttgcca
     4861 ggacgccaag aataacaatc accaccatca cttccagcag ggtaaaacct gcccgtggtt
     4921 tttgtgtgcg ggataacgaa ttcatgtgat aactccgtaa attacattcc aaccatattg
     4981 ttcagttgca gcatcggttc gaggatggcg atgacgataa acagcaccac gcccgccatc
     5041 atcaccacca gcgccggctc aaacagccct aacgccagcc ccacctgggt atcaaattcc
     5101 cgttcctggt tgaccgcggc ctgttcaagc atggtttcca gctcgccgct ctgttcgccg
     5161 gaggcgatca tgtacagcat catcggcggg aacaggcgca gatccgccag cgcggcgcgc
     5221 aggctgcttc cttcgcggac gcgatctgcc gccagcagca gttgttgctc gacataacga
     5281 tttgccgaca cggcggcggc ggtctgaatg ccttccagca gcggaacact gctggcggtg
     5341 aggatgctta acgtgcggga aaaacgcgcc gtattcagtc cgcgcgcaac acgccccgtc
     5401 acgggcaaac gcagcaaggt tttatcccag cgcaggcgca tcgcaggatt tttgagtagc
     5461 cgttgcccca gcaccagaag ccccagcaaa cccgccagcc agtacacgcc gctggcctgt
     5521 aacgcgtcgc tcatagcgat gagcattcgg gtggaggcgg gtagcgcgtg tccgagatga
     5581 tcaaactgtt cgataatttt cggcactact gccgtcagca aaatagtgac cacgcccgtt
     5641 gccaccacca gcagtaccag cgggtagagc atggcctgca gcaggcgtga tttcaggcgc
     5701 tgtcgctgtt cggtgtaatc cgccaggcga ttgagcacca cgtcgagatg cccggatttt
     5761 tctccggcag cgaccatcga acaaaacagg gagtcaaaga cgcggggatg ttcgcgcagg
     5821 ctgtccgaca gggtataacc ttcctgaatc cggctgcgca gcgccattcc gaggcttttt
     5881 acatgcagtt tttcgctttg ctcactgacc gcctgtaagc aggtttccag cggcattgct
     5941 gcctgcacca gcgttgccag ttgacgagtg aacagcgcca gatctgccgc cgccacgcga
     6001 cgatgtgcgt gccgccgacg ctgcaacaat ccccctgccg atgcattcat ccgggcttca
     6061 atgtgcacgg gaatgaggtc tttaccgcgt aacaattgac gggcatgacg cgcggaatcc
     6121 gcctcaatca tgcctttggt tttgcgacca ttacgctcca gcgcctgata gtaaaacagt
     6181 gccattacgc ctccatggtt acccgcagaa cttcatcgag agaggtttct ccggcgagca
     6241 ctttctcaat gccgttactg cggatacccg cagagtgttg ccggacataa cgttccagct
     6301 ccagttcccc ggcctgacgg tggatcaaat cacgcaatgt ggcgtccacc acaatcagtt
     6361 catggatggc agtacgtccg cgaaaacctt tgtgattaca ggcggggcag ccctgcggat
     6421 ggtacagcgt gacggcatgc gtatcggaga tccccataag acgtttttct tcgtcggtgg
     6481 caggcgcggc ctgacggcag tcggagcaca gcgtgcggac cagtcgctgc gccatcacgc
     6541 ccgtcagact ggaagagagc aggaaaggct caacgcccat atcctgcaaa cgcgtgatcg
     6601 cccccaccgc cgtgttggta tgcagcgtgg aaagtaccag gtgtccggtc agtgaagcct
     6661 gaacagcgat ttctgcggtt tcggtatcgc ggatttcacc gaccatcacc acatccgggt
     6721 cctgacgcaa aatcgcgcgt aggccacggg cgaaggtcat gccgacgcgg gtgttaacct
     6781 gcgtctgacc gatcccttca atcatgtatt cgatagggtc ttcaaccgtg agaatgttac
     6841 gcgattggtt gttcagctcc tgcaatccag cgtacagcgt ggtgcttttg ccggaaccgg
     6901 tcggccccgt caccagaaaa atgccgtgcg gtttgtgtaa cagctggcgc aactgcgcag
     6961 tcagttcgtg actcaacccc agacgttcca gcgtcaggcg ggcctggttt ttgtccagca
     7021 gtcgcagcac cacgcgctcg ccccaggcgg agggcatggt ggagacacgc acgtcaatcg
     7081 cccgaccgcc cagcagcagc gcaatacgcc catcctgcgg tacgcgcttt tcggcaatat
     7141 ccagccgcgc catcaccttg atacgcgata ccagcagcga ggccagtttg cgtcccgggc
     7201 gcagcatttc atgtaatgtg ccgtcaacgc gaaaacggat caccagactc ttttcaaatg
     7261 tctcgatgtg gatatccgaa gcaccttctt taatcgcctc tgccagcata gcgttgatca
     7321 gtttgatgat tggcgcgtcg tcgtcgcttt ccagcagatc ttccgtttcc ggcagttctt
     7381 cagcgagggt aaaaaagtcc tcggcggagc cgagatcttc catcaactgg cgagcctcgg
     7441 aggagtcccg ctgccagacc gcattcagcc gctgttcaaa ttcggcctcg tcgatttgcc
     7501 gcagcgtaaa gggcgcgttc agcccccgtt gtagctcctg caaaacagag agcgacaacg
     7561 gatgaacatg gaggatctcc agcgacgctt cgcaccatgc caccaggcta aaccgacggc
     7621 tgaaactgta gggcagacgc acggtgttag cggtggtttc ctgtgctaca ggcaccatta
     7681 acgcgttctc ccggcattga ggaacgcacg aacttctggc ggtaaggcct gattttgcgc
     7741 aggcaatatc ggctgcgcgg tgtgcggcat caggcttaag ccttgctcat cgcggtagat
     7801 ttgctcggcg cgcatgtagt tatatttgcg ctgcgacacg ccgtctgccg ccataccgtc
     7861 acgcagaatg gtcgggcgga taaacaccat caggttacgt ttttcttttt tatccgccgt
     7921 cgatttaaac aggttaccaa tcaacgggat atcacccagc aacggcactt tcgccacgct
     7981 ttctcccgcc tggtcgtcca tcagaccgcc aagcacgatc agctcaccat cgttagccag
     8041 cacggtggtt ttcagtttgc gctcgccaaa cacgacatcg aggctggtct gcccttccac
     8101 tttcgagact tcctgctcaa tcaccatctg taccgcgtta ccttcgttaa tctgcggcgt
     8161 gactttcagc atgatgccga cttttttcct ctctaccgta ttgaaaggat tgctgttatt
     8221 ggagccaacg gtagatccag ttaataccgg aacgtcctgg cccaccatga agaaggcttc
     8281 ctggttgtcc agcgtggtga tactcggcgt ggagagcacg ttcgagctgg agtcgttttt
     8341 gaccgcctgt accagcgcca tccagtcgcc tttcaccacg ccaaccgccg taccgctaaa
     8401 gccagaaaga agctgagcga gcgtggagag atcgccgtta gtatccggat ttatggtggt
     8461 agcaccgttt tcgctgatca ccgtggaacc tttctgcggc ttggctgcag aaatcgctgc
     8521 ccccagcgta ccaataggga tttgcgtacc gttggcaaac tgcattaatc cggcatcttt
     8581 tgacccccac tgcacgccga agttgatatt gctgccttcg gcaacttcca cgattaacgc
     8641 ctcgacatgc acctgagcac ggcgaatatc cagttgttca atcacgcttt gcagcgactg
     8701 cataatgtcc tgcggcgcag taacaatcag ggcattactg tgtttgctgg cggcgatgga
     8761 gacaacctca cgcccgctac caaccgtgcc ttccgcctct tctttagccg ccgtgagcgt
     8821 accgctgacc tgcttcagca catcgaccag atcttccgct ttgctatatt tgagatagaa
     8881 cacctggctg ttgccgctgc gctccatttc tgaatccagc cgacggatca gacggcgcat
     8941 tttgtctcgc gtggcggggt caccactgac aatcacgctg ttggtgcgtt catcggcgac
     9001 aatttgagat ttcagcgttg ctggctggtt ctcgccgctg tttttggtca ggctttccag
     9061 cacgcgggca atttccgagg ctgaggcgtt atccagcggg atcacctctt cggcgcgatt
     9121 acccgcgtga tccacgcgct ggatcacttc cgtcaggcgc tccacaactg aggcgcgtcc
     9181 ggtaagcata atcacgttgg agggatcgta attaacaacg ttgcctgagc ctgcgctgtc
     9241 aatcatctgg cgcagaatcg gtgccagctc gcgtaccgac acattacgta ccggcacgac
     9301 tttggtgacc atttcatcgc ccgcgtagtt gtcgctgcct tcaccgacca gcggcagcgg
     9361 ctcgactttc gcggcgcttg atttcaccac cttcagcacg tcgttttcca tcggtacgac
     9421 cgcatacccc tgtgcttcca gcaggttaag gaataactgg taatactggc gttcattgag
     9481 cggagtcata gtgcgaatac tcactttccc ctgaacgccc ggccccataa tgatggtttt
     9541 attaaggtta gcgccgacgg tttcgatgaa cgatttcagg tcggtatctt taaaattagc
     9601 ggtgaaagtg gcttcttccg cccagaccgg tgaactacac aatgccgctg ccagcatcag
     9661 cggcagtaaa cgcttttttg ttttgaggcc agttgtcttc ttacgccaga tagacaacgt
     9721 catatcacgc caaaacacga tgaatgattc tcctggatta aatgcggtta gcgcagtgcg
     9781 atggaaatgt cgtagcgcgc acccttgcgt aaaaccgtaa gttgaatgga atccattgaa
     9841 ggtaactgcc gcatcagagc aatcattgct cgtggatcag tgaaatcctg ctgatttagc
     9901 gcaatggcga tatcgccttc cttaaaaccg ctggcatcga acagagaacg atctgccccc
     9961 ggtttcactg cataaccgac aatcccctcc ttacgcacag gcgtaagctg gatatagtta
    10021 aaaattttct gcggatcttt cgccagtgcc tgacgcacgg cagccgggat ctccactggc
    10081 gcactgacga caggttcagc aacagcttgc tttgcttcgt cactggcggc ttttttgctg
    10141 gtcaccgcca caggggggcg ttcctcctct gccagactca gacgttccat tttcccctga
    10201 tagcgcagca tcacatggtc acgattaatt tcctcaatca ctgcgttgtg agagccaagc
    10261 cgttcaccct gcaaatagac ctgctgtttg ccgccttctt caataaccgc gccgggtctg
    10321 gcaccaaagg cgatcccgcg cagcaccaca ttaagacgcg tttctgccac aggcgcagat
    10381 tcaggttgtt ttaccggcgc ggcgacgggt tgatatttgc caaaccagtt ttgttggctg
    10441 actaattgca cgtcattttt atcgaacggt tttgcatccg cacggagcgg tttattcgcc
    10501 gatgaggaaa ccgccgtata ttccgcagaa aaggagaaat agcgccagag tgaatacgcc
    10561 atttttgcag aaataataag cagcatcagc cagaacatcg cgcgtgcaat tttgcgcaga
    10621 tgctctttgt ctgtattaag agattgtctc tggctaagag tgtgacgaat tttagtcagc
    10681 cattgaatga gataaattcg tgcgtcacga aaaacaaccc gcgccaattt atatctaccc
    10741 gacgttatgc tttgactatt ccacaggtgg tacgatccag tttccgctga tactggttac
    10801 ctgtacgtgt atcattaatc gttatgctgt aattaattcc ctcggtgatc attaatttca
    10861 ccgttgggtc agcgcacatt tgtcgttgat agctggttaa aaaggcgtca ggtgtctgcg
    10921 tggtctgagt acccgattcg ctgataatgg tcatttttac cgtcgtgcca ctactttgcg
    10981 ccagcactaa ggtatatccc gcagatttaa tcggcaggtt ttggctgata ttttgcgcct
    11041 gttttttcgc cagtaaactg gcgttttcat tatggctggc gcagccactt aataatcctg
    11101 taacgctcaa caatagcgat attaataccc tccctggcat ctgttttatc gacatggtaa
    11161 ataatctcta aggttattaa taagagttaa aatgtcactt tgataatgac gtggttatca
    11221 ttaaaacaat gcctgtagat aaagtgttgc tataccgccc agacttaaac acggtccaaa
    11281 aggcagtgtg gttgatcctc tttttgtaat aacggcatat atcagaccgc agcatgatgc
    11341 gattaaagcc acattgggta gcgacaacgg ccccacccag ccacctaaag cggcgaagag
    11401 taatacatcg cccatgccta atgcttcttt acgcagaact attccggcta tccagcgcag
    11461 ggagtaaaaa gtgataaacc ccaccagaac gccggtgact gcgtcttgta gcgttaacgg
    11521 actctgctgc gcccatgccg caatcagtcc tgtccacaat acgccctgag taaaaacatc
    11581 gggcagccat tggttgtcga ggtcgatgat gctcgcggca atcagccacg cggataatat
    11641 catcaccgcc agtccccatc cactttctgg ccacaccaga ctcgccagca aaaaagcgag
    11701 tgctgtcaat aattccacca gcggataacg cttgctgatt ttcgcctgac agtcacggca
    11761 gcgccctttg agcatcagcc aggagagcag cggaatattg tcacgaacgc ggatagtctg
    11821 ctgacaatgc ggacagtgcg aacgcggtag cgcaaggctt atttttgact gcgcactcgg
    11881 catttcacca tgaaactccg ccatttgttg gcgcagcatg atggggtaac gccaaatcac
    11941 cacattcaaa aaactgccga tgatcaatcc tccgacggtt gccagtatgg gcatcgccgc
    12001 ggggtattgc tgaaaaacat caaaaagcat ggttaaaggt tgtttgttgt aacttgccgg
    12061 atgcgggcct gcgggtgtat gccatatggc tttcctccag gcccgataag ctggcgcatc
    12121 gggcatatgt gttgacccga tgcgccttat atcatgccgg atgcggcgtg aacgccttat
    12181 ccaggttatt cagcaggcat cgaatattcg gtaaccttat taatggtttc cggcccctgt
    12241 tccggtttcg gcagcttgag cgacgcgagc gtgttgtaag ccgactggct cacaccgccc
    12301 tcgaagctca tctctgccgc tcccggtagc tggtaagcat tcgcacccgg attccatttc
    12361 ttaaagaact ccgaaagatc cgtctgggcg acccaggagg cacacagcat cagcgtgtcg
    12421 gcagcgttac cgttggattc agcacagtaa ttcttgccac caaaggtgct gttaccaaca
    12481 tcatcgccgc gtgctttacg atgcatcaac tggaacaggt tccagccttt catcccttca
    12541 cgctcgctgt agaacgctgg cagcttgcca tctggatacc acttcgtgat atcaaagttt
    12601 ttctctgccc actctttcag ctgtgcgtac atcagcagac ggtcccccgc accgccgcgc
    12661 gcccaagcct gaccgttgct ctcgtccaga tattccggcg cgacggtaat gtcgtcagcg
    12721 acacggttca tcttgccaag atagcgatcc tgcatgtaca gcgccagcac gttgttcgcc
    12781 acttcagttg cacccggtac gttcagcggt gtttctgcag cgttatgacc gacttcgtgc
    12841 caaatcagcc agtcgttcag cggcgtcgtc ggcagcgtgg tgctgttcgt cgagaagctg
    12901 ctgttcatta ccggataacc cgagtgcgca tcaccgatgg agatctgcac atcgttggcg
    12961 aaacggtgtt tatgccccgt caatgcttca taggtaaaca tccggtggtt accgctcgtt
    13021 tcattgcgac cgtagaagtc attcatcgag ctggcaaagg tatccagatc tttagcgaat
    13081 tctgctacac caccagtgaa attgctggcc tcaaggttct tcttcggcgt ggtatagacg
    13141 aacgacgcag actccagctc gcccagcggc gccggtgagt ccagatcgtt tttccattcg
    13201 ccgtctttat agaacggcgc ttttactaca ccggtaaagg tgaagttagc agaaacatca
    13261 tccttactgt cgcccttgat ataaatcaga ccaccataag gcaccttgaa ggtcacttca
    13321 ccgttagcct ccagagtata cgttttagtc actcttggcg gacggttcag cgcaacttca
    13381 tgcttctcac gtccggtcag gtcgtcagcc agcgccacgg taacagtcac tgggactgac
    13441 gccgaagact taatggtgac gtcctgctgg gccggtgccc acaggccggt tgactgcatg
    13501 ttacccgcaa accatttggt cggattcgag tacaggctga tgttttccgt aacgctctca
    13561 ccctttgccg atacggatcc tgggtacttc tccacatcaa ccttaatgtt cagatcccac
    13621 caggaacggc ccagcatcag acgcgtcagc ggtttttcca tatagttgag cggatagctt
    13681 gggttcatca tgcccgcttt gctgctaccg tcaccgtaga tcatgttgtt atcgaccagc
    13741 gattttttca gatctgcgga gcacttggtg ccgcctgcat aggcatcatt ggcgtagcag
    13801 ttcaggaact cggtgaacgt tttaaagccc agctcatctt ccttgccctc ttcgtaacga
    13861 tatttcgtat cgttccacag ccagaccgac atgttctggt acagacgttc cagatcaaca
    13921 ctgttcagac gctcaccttt actgcctttg gtacggaaat aaagctcatg ctgatacagg
    13981 cgctgaatgt tggtgcctaa atccgccgcc tgcaccatcg ctttcgcggt gtcggcgtca
    14041 agattcagtt gcgtatagcg cggaacatac atgccacctg ttaccggaac atccgtgcct
    14101 gggcggcgct ccaaacagtt aatctcgtaa tggtaagtcg agtccttaca ctcctgtaac
    14161 ccaggaaact tctcaaagat ttttgccttt gccgcttcca gagattcttc tgttgtgtat
    14221 tccgcttcat caataaaggc ataacgcgtt acctgtttgc cctcaacttc ctcctgccag
    14281 ctcgcaactt ccagtttcgg cttgtcatca ggcttgttgt cttgctggta tttccaggtc
    14341 acttcccctg tatttgggtc gatggtgtac ggcggttgcg cgccgtctgc agcaggataa
    14401 cgttcataaa cccaaatgcc agtcgcgcga cgctgacgaa cgcgatccgg atacccttgc
    14461 ggatcgttgt tcaccaccga tttgttcaga gccattgaca gacccgcggc atccagcaga
    14521 cgcacaaaac tggacgcgct ctcttcctta agattgctca tcacgttttc catgatcagc
    14581 accgagccac ctttgttcag ataagcgatc agatcggtca catcctgctg agtcagcttc
    14641 ggtttgctgg tatctgcacg cagaggcaca gcatagggat cgccagacca ctgagtcaca
    14701 tattcaaagc cgttgaggat cagcaacgga atctcttccg gattcagatc gccatagctg
    14761 gtcaactgtt taaccgtgat accagtgaaa tcctcatgga aagcaaatgg tgcactattt
    14821 cccaatacct ggcccgcttt tttgaaataa acgttctcca ggttggtgcc gacagtcatg
    14881 atgctcttgg tatttggctg ccagatgtca tttgacaagt agcgcaggac gttctgcata
    14941 aagtgcttca tgtcatcaga atcaccgctg agcgtacact cacctttact attaacacca
    15001 ccgccccaac tgtaaccgtt cgggcaacgc aggatgctgt tgtagtgcgg gttaccgata
    15061 accatcagtt tgccttcacc gacttgcccc agcgaaataa acggcaggtt gaaagtcgca
    15121 gtatcgcgcg taacgttctc tggctgcaca atggaaggcg cttccgtaat gtacgccagc
    15181 tcatttttat cccaggcgcg tttttcgcca aacgccagcc agtagttttt atcattacgc
    15241 gccatcagaa tcgggaatgc cgagttggag atatttacca ccgcctgacc gcgcgcgtta
    15301 ccggtgctgc catagaagtt ggtagagtca tggaagacgt ggaacttgct gacagactga
    15361 tagttcgtat ccacgcccca cagcttgtta ataacgccct gaatctgacc gtcattaaca
    15421 ttgcgtgtcg tcagtgagaa ccagcgagcc tcgttacaac cgtcggtttt cgcacaaatc
    15481 gcggtatcga tctctttggc ctgacccgtc ttaaactgct cgataaattc gttaggcagc
    15541 acaacgtttt gatcgccttc atccagcgtc gcaccgttgg ataacgaaag attgattatc
    15601 tcgttgatca cgttgggata ttcggcaaag accttgcgta catcgtccgg aacaacacgg
    15661 gtattatttt gaccagtcgt cgaataacga tgaatgagct gatcgatatt tgccccgcga
    15721 acttcatcac ccaattcagt cagcgcaatg gtcgacttat tgccacgtac tgagcccagt
    15781 tcaaaggtat cgataccaaa ggagatggtt tcgccccagc taaaggagaa tttaccgttt
    15841 tcgtcagtta cgccacggcc tgaattggtg tagtagtcaa cgccagcaac accgttcccc
    15901 aggctatcca ccagttggcc ttcggaaaga atgatttcag tgggttgata ctgataaaac
    15961 tgttccgcgt tagccgacac gaaggacgcg ttcagatccg gttttgttcc ctcagtcgtg
    16021 actggcacta ccgttgaggt atgggtggac ggcgctttat cagtcgcagc attgttttcc
    16081 acctcttcat tgaccagctt gctgaaattg tctgttgcca gatcaatttg cttatacagt
    16141 ttttcaaatc gcgcgcgatc aaccactgac gagaaagtaa gacaaagctg ttctgcatct
    16201 gcggggcaac tgtcgctgga cgtcaccaga gagatggcgt tggttttctt attttcagaa
    16261 ttcgccagct cctgcgcgtc ctccaggcta aacgacactt tgtcaaccgc acgcaggcta
    16321 cgcgcagctt ctgactgggt gttgaatgtt gcaatggtcg tactgcccac cacacaactc
    16381 acggtattgc ctggcgtaaa ggtaaagcca tcgctggatt caccattaca ggtagcacca
    16441 gttacccgct ggcttccgcc cagggtcaga taacccgttt tcgtaggaac aggttctggc
    16501 tccggttctg gtgtcggctc aggatcaggt gttggatccg gcgtaggttc tgggtccggc
    16561 gtcggctcag gtgtcggctc cggggttggt gttggatcgg gtttcacttc cggcaaagtc
    16621 cctgatccag aatctacaga cggcgtatcg gaggacgatc ctgaaccacc accatcacaa
    16681 ccggctaaca gggttgcgct taaa
//
LOCUS       DADTFG010000110        16652 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.111, whole genome shotgun sequence.
ACCESSION   DADTFG010000110 DADTFG010000000
VERSION     DADTFG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.111"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(198..2630)
                     /locus_tag="J1138_003304"
     CDS             complement(198..2630)
                     /locus_tag="J1138_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBA8455066.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLEHLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(2633..3793)
                     /gene="metB"
                     /locus_tag="J1138_003305"
     CDS             complement(2633..3793)
                     /gene="metB"
                     /locus_tag="J1138_003305"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBA8455067.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEHTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4070..4387
                     /gene="metJ"
                     /locus_tag="J1138_003306"
     CDS             4070..4387
                     /gene="metJ"
                     /locus_tag="J1138_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBA8455068.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            4447..5055
                     /locus_tag="J1138_003307"
     CDS             4447..5055
                     /locus_tag="J1138_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBA8455069.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5116..5328)
                     /gene="rpmE"
                     /locus_tag="J1138_003308"
     CDS             complement(5116..5328)
                     /gene="rpmE"
                     /locus_tag="J1138_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBA8455070.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            5531..7729
                     /gene="priA"
                     /locus_tag="J1138_003309"
     CDS             5531..7729
                     /gene="priA"
                     /locus_tag="J1138_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBA8455071.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            7885..8910
                     /gene="cytR"
                     /locus_tag="J1138_003310"
     CDS             7885..8910
                     /gene="cytR"
                     /locus_tag="J1138_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBA8455072.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9002..9961
                     /gene="ftsN"
                     /locus_tag="J1138_003311"
     CDS             9002..9961
                     /gene="ftsN"
                     /locus_tag="J1138_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBA8455073.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLVQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARVADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10054..10584
                     /gene="hslV"
                     /locus_tag="J1138_003312"
     CDS             10054..10584
                     /gene="hslV"
                     /locus_tag="J1138_003312"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBA8455074.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            10594..11925
                     /gene="hslU"
                     /locus_tag="J1138_003313"
     CDS             10594..11925
                     /gene="hslU"
                     /locus_tag="J1138_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBA8455075.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQIEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            11992..12918
                     /gene="menA"
                     /locus_tag="J1138_003314"
     CDS             11992..12918
                     /gene="menA"
                     /locus_tag="J1138_003314"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBA8455076.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     IVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13011..13496
                     /gene="rraA"
                     /locus_tag="J1138_003315"
     CDS             13011..13496
                     /gene="rraA"
                     /locus_tag="J1138_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBA8455077.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(13581..13826)
                     /gene="zapB"
                     /locus_tag="J1138_003316"
     CDS             complement(13581..13826)
                     /gene="zapB"
                     /locus_tag="J1138_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBA8455078.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            14251..15096
                     /gene="glpF"
                     /locus_tag="J1138_003317"
     CDS             14251..15096
                     /gene="glpF"
                     /locus_tag="J1138_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBA8455079.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15119..16627
                     /gene="glpK"
                     /locus_tag="J1138_003318"
     CDS             15119..16627
                     /gene="glpK"
                     /locus_tag="J1138_003318"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBA8455080.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
BASE COUNT         3947 a         4427 c         4463 g         3815 t
ORIGIN      
        1 ttgttactta atgtcatttt gtaattgtca ttaataactt caccattcac tctgttactt
       61 catcgctcaa cacaaaagcc cccttattac cgggggcact gaccaaaggc aaaatgacaa
      121 tcaatcacca cttccccagg aagaggttta ggggtgacaa caaaagctgg caactgccag
      181 cttttgttca ataatcacta caacaactgc gccagccggt taatatccga ctgaatcgcc
      241 ccggcggtga cgtcgcgccc tgcacctggc ccgcggatca ccagcgggtt gtcgcgatac
      301 cagcggcttt cgatggcaaa gacgttatcg catggcagta gtgatgccag cggatggtct
      361 tcacgcaccg cttccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtaa
      421 cgcagcacca gccccatttc gcgggccgct tccagtcgtt gcaccatctg ctcgttaagt
      481 tcatcgccat tttcaaagaa atggtcgatg ctgccgcctt cgcaatgggc aggtaccagt
      541 gattcaacac gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc
      601 agcttgcgca tcacgtcttt gccggaaagg tcatcacgcg ggtcaggctc ggttaagccc
      661 tgctgccacg cctgatccac cagttcggta aacggcacgc taccgtcgaa ttgcaggaac
      721 agccaggaga gcgtgccaga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg
      781 atcagatcgc gcacggtatg gttgatcggc aaacccgcac cgacagtggc attgtacagc
      841 cagtgacgcc cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctg
      901 gctcctgcca gtttgttggc gctgatgacg tggaaaccgt ggctggcaaa atcgagatac
      961 tgatcagcaa gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg
     1021 gcgcgcatcc acaggaacag cgactcttca tcctgctcga ccgcttcatc gttgaagaag
     1081 gctaacgcgc ggctggcgtc caggccgtca tagctcaaca ggctgcggcg gctgtccacc
     1141 acacctgcca gcacaaattc aaagccggta cgtgccgaaa gcgtgctctg ctcacgggcg
     1201 aacagttcca gccagcggga accgatattg cccttgccga acaataccag gccgatgcgt
     1261 ttttccgcac ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg
     1321 cgcagtactg ccaccaggct gatgccatca tcagactgcc aggtaaactc gaccggctgg
     1381 cctttcagtt gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac gcccgcccca
     1441 accatcgcca ccagcgccag cccctgacgc aggcgcagtt cgccaggtaa tcccgcttcg
     1501 tcgaggattt tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg
     1561 cgatcgttat gcacgccaac cgccagtggg cgcacctgag cacgtttcag gatctggtcg
     1621 atctctttat gcgccagttt gaaatcctgg ctggcgggca cctgaaactc aatcaagcag
     1681 acatcatcat ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg
     1741 cgcgtggaac cttgatccgg cgtgtagcta cagcgcagtt gcaggtcgat ttcgctgcca
     1801 gaaacgggct gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg
     1861 gcttcatcca gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggcg
     1921 ctatataccc cggcgacgtc gctccagatg gttacgcgag aaacacccgc cagcgcaccg
     1981 atttgtgtcg cggaatagtc ggaaccgtta cgccccagca gcaccgtttc accggcgttg
     2041 ttacggctga taaacccggt caccaccaga cgtttgcccg gatgttgtac cagtagctgt
     2101 tgcagcaacg ggtaagaaag tccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt
     2161 aaaaactcgc gggcatcaag ccaggccgct ggcagtcctt gttgattaag caccgcagac
     2221 atcaggcgcg ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta
     2281 ataccgctgt cgagcaacgc cgccaggtgc tcaagatcgc tgacaaaagc gctaatgagg
     2341 ttatcggctt cttcggcggg tagcaggccg ctaatcagat cgcactgata gcgacgcagc
     2401 gtttgttgaa cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc
     2461 aactggttag tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagtac
     2521 tccgccataa tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca
     2581 aatttatgca gttgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct
     2641 tgtttgcagc ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac
     2701 cggtggagat acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggcgcca
     2761 tgcctgcatg tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt
     2821 ccgccagcgt aaacaacgac agcccgccca ggaaacgacg cagcgtgtgc tcatcgccat
     2881 ccagttcaaa actcaacatt gcgccaaagc ctttttgctg gcgcgcggca atttcatgcc
     2941 cctgattttc cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat
     3001 atttcacaat cgcctgcgcg ttgcgctggg ccagctccat tcgcggcacc agcgttcgca
     3061 acccgcgcag cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc
     3121 accaggcaag ttcagtgaca acgtccgggt ctttagcaat cactacgcct gccaccacgt
     3181 cagagtgacc gttcagatat ttcgtgcatg aatgcaacac cagatcggca cctaatgcca
     3241 gtggattttg taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt
     3301 cccttgccag atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc
     3361 tttctaccag taccagtttg gggttttctg ccagtgctgc ccgtaatgcc tgttcatcgc
     3421 cttgatcaac aaacaacacg cgatagcaac cgcgtttcgc caggctgtcg aacaggcgat
     3481 agctaccgcc gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg
     3541 tcgttaccag gtgaatcgcg gacatgccgg tattagtaag tactgcacca gcaccgcctt
     3601 ccagttctgc cagcgctcgc tgaaccacat cgcgcgttgg gttgccgcgc cgcgagtaat
     3661 catgcgcgcg cggttcatta aatccggtaa agttataggt actggaaaga tggatgggag
     3721 ggacaacgca accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct
     3781 gtttacgcgt catgtgatga agttccctgg gcttggtcgg tgaaatgtca ggcaccagag
     3841 taaacattgt gttaatagac gtcaatacat ctggacatct aaacttcttt gcgtatagat
     3901 tgagcaaatc tcaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac
     3961 ccgatgtcac ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag tacccatatt
     4021 aattgtgtgg tctggcctca atttattgac gaagaggatt aagtatctca tggctgaatg
     4081 gagcggcgaa tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa
     4141 aattacggtt tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg
     4201 tcaggtgaac aacctgcgtc acgcgaccaa cagcgagctg ctgtgcgaag cgtttctgca
     4261 tgcctttacc gggcaacctt tgccggatga tgccgacctg cgtaaagagc gcagcgacga
     4321 aattccggag gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga
     4381 atactaaagc gaaaaagagc ggcgcggagt taaccagccg cttttttcat acatggacgt
     4441 ttaacgatga aaatcaggct actgattctc agcctgctgg tttctgtgcc tgcatttgcc
     4501 tggcaaccac aaaccggcga catcatcttt cagatctctc gctcatcgca aagtaaagcg
     4561 atccaactgg cgacccattc cgattacagc cacaccggta tgctggtgat acgcaacaaa
     4621 aagccctacg tttttgaagc ggtcggcccg gtaaaataca ccccactcaa gcagtggatc
     4681 gcccatggtg aaaagggcaa atacgttgtt cgccgcgttg aaggcggact gagtgttgaa
     4741 caacagcaaa aactggcgca aacggcaaaa cgttatctcg gtaaaccgta tgactccagc
     4801 ttctcgtgga gcgacgatcg ccagtactgt tcggaagtgg tgtggaaggt ctaccagaat
     4861 gcgctgggaa tgcgtgtggg cgagcagcag aagctgaaag agttcgatct tagtaacccg
     4921 ctagttcagg caaagctcaa agaacgctac ggcaaaaata ttccgcttga ggaaacggta
     4981 gtttcaccac aagccgtttt cgacgcgcca caactcacca cggtcgccaa agaatggccg
     5041 ctgttttcct ggtaatacga ccaaaattca ggcacaaaaa aagcgccgtg cggcgctttt
     5101 ttcggaaatc cggtcttatt tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac
     5161 acggccaccg gtagcaacat cacgctgttt gccagtgaag aacgggtggc acttgctgca
     5221 cacgtcgagg ttcaggtcat gaccaacggt ggagcggatt ttcattacgt taccgcaaga
     5281 gcagctagca gtaatttctt cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg
     5341 gtttaaggcc gcgtcgctct tccagcccta acgccagaca ccacgcgatg ttaaaagtat
     5401 agcttcaata cgatcatttc gtacgaaggc gcgaaatcat acagaaatta accagcgtat
     5461 gcaaactgat ccgcactctt ctacggcaat gtgtatacta acccaccgaa tttcaagtca
     5521 ggatgatgct atgcccgttg cccacgttgc cttgcccgtt ccgcttcctc gtacctttga
     5581 ctatctgctg ccagaaggca tgacggttaa agccgggagt cgcgtgcgcg tgccgtttgg
     5641 caaacaacag gagcgtatcg gtgttgtggt gtcagtgagc gatgtcagtg agctgccgct
     5701 taacgagctg aaagcggtag ttgaagtgct ggacgttgag ccggtattta ctcactctgt
     5761 ctggcgatta ctgctctggg cggcagatta ctatcatcat ccgattggcg atgtgctgtt
     5821 tcatgccttg ccgattttac tgcgccaggg gcggcctgcg gcgaacgcgc cgatgtggta
     5881 ctggtttgcc actgaacaag gtcaggcggt ggatctgaac agcctgaaac gttcgccaaa
     5941 gcaacaacag gcgctggcag cgttacggca aggcaaaatc tggcgcgacc aggttgccac
     6001 gctcgaattt aatgatgccg cgttgcaggc actgcgcaaa aaaggtctgt gtgatttagc
     6061 aagtgaaaca ccagagttta gcgactggcg aacgaactat gccgtttctg gtgagcggtt
     6121 gcggttgaat accgaacagg ccaccgccgt tggcgcaatt catagcgcgg cagatacttt
     6181 ttctgcctgg ctgctggcgg gcgttaccgg ttccggtaaa acggaggttt atctcagtgt
     6241 actggaaaac gtgctcgctc agggcaaaca ggcgctggtg atggtgccgg aaatcggcct
     6301 gacaccgcaa actatcgccc gttttcgtga acgttttaat gccccggtgg aagttctgca
     6361 ttccggcctg aacgacagcg agcgtctttc ggcgtggttg aaagcgaaaa atggcgaggc
     6421 ggcgattgtc atcggcaccc gctccgcgct gtttacgccg tttaaaaatc tcggcgtgat
     6481 tgttattgat gaagagcacg acagctccta caagcagcag gaaggctggc gctatcatgc
     6541 ccgcgacctg gcggtgtatc gcgcgcatag cgagcaaatc ccgattattc ttggctccgc
     6601 aacacccgcg ctggaaacgt tatgcaacgt ccagcagaaa aaataccgcc tgctgcgcct
     6661 gacgcgtcgg gcggggaatg cgcgtccggc aattcaacac gtgctggatt taaaaggtca
     6721 gaaggtgcag gctggtctgg ctccggcgtt aatcacccgt atgcgccagc atttacaggc
     6781 caacaatcag gttatcctct ttcttaaccg ccgtggcttt gcgcctgcct tgctgtgcca
     6841 cgactgtggt tggatagccg aatgcccacg ttgcgatcac tactacacgc tgcatcaggc
     6901 gcaacagcat ttgcgctgcc accattgcga cagtcagcgt ccggtgccgc gccagtgccc
     6961 ttcttgcggt tccacgcacc tggtacccgt ggggctgggc accgaacagc ttgaacagac
     7021 gctcgcgccg ttgttccctg gtgtccccat ttctcgtatc gaccgcgata ccaccagccg
     7081 caaaggggcg ctggaacagc aactggcgga agtgcatcgc ggcggcgcga ggatcttgat
     7141 tggcacgcaa atgctggcga aaggtcacca ctttccggat gtgacgctgg tggcgttact
     7201 ggatgtggac ggcgcgctgt tttctgccga ttttcgctcg gcagaacgtt tcgcccagct
     7261 ttacacccag gtcgccggtc gtgccgggcg tgcgggtaaa cagggcgaag tggtgctgca
     7321 aacgcaccat ccggaacatc ctctgttgca aacattgctc tataaaggct acgacgcctt
     7381 tgccgaacag gcactggctg aacgacgcat gatgcagctg ccgccgtgga ccagccatgt
     7441 gattgtgcgt gcggaagatc ataacaatca gcacgcgcca ttgttcctgc aacaactgcg
     7501 taatctcatt ctcgccagcc cactggcaga cgagaaactg tgggttctcg gtccggttcc
     7561 ggcgctggcc cctaaacgtg gcggtcgctg gcgctggcag atattgttgc agcacccttc
     7621 ccgcgtgcgc ttgcagcaca tcatcaatgg tacgctggcg cttattaata caataccgga
     7681 ttcccgcaag gtgaaatggg tgctggatgt cgatccgatt gagggttaaa ccgctcacga
     7741 tgcgaggcgg atcgaaaaat tcaatattca tcacactttt catgaaaatt ctgtaaccgt
     7801 tttcacgcgc tatctgctaa aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc
     7861 tgacgtgcca ggcgaggagt gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg
     7921 aaagacgttg ccctcaaggc aaaagtctct acagcgaccg tctcccgagc attaatgaat
     7981 cccgataaag tctcccaggc cactcgtaat cgggttgaaa aagcggcccg ggaagtgggt
     8041 tatttaccgc agcctatggg gcgcaacgtc aagcgtaatg aatcccgcac cattctggtg
     8101 attgtcccgg atatctgcga ccctttcttt agcgaaatta ttcgcggtat cgaagttacg
     8161 gcggcaaatc acggatatct ggtgctgatt ggcgactgtg cgcatcaaaa tcagcaggaa
     8221 aaaaccttta tcgatttgat catcaccaag caaattgatg gcatgttgct gctgggttca
     8281 aggctgccgt ttgatgccag cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg
     8341 aacgaatttg caccggaact ggagctacct accgttcata tagacaatct gaccgccgca
     8401 tttgatgcag taaattattt atatgagcaa gggcataaac ggattggctg tatagccggt
     8461 cccgaagaga tgccgctgtg tcactaccgc ctgcaaggtt acgttcaggc gctgcgtcgc
     8521 tgcggcatta tggttgatcc gcaatacatc gcccgtggcg acttcacctt cgaagccgga
     8581 agcaaagcaa tgcaacagct gcttgatctt ccacaaccgc ctactgctgt cttctgccat
     8641 agcgatgtga tggcgctcgg cgcactttct caggcaaaac gtcaggggct gaaagtcccg
     8701 gaagaccttt ccataatcgg ttttgataac atcgacctga cgcaattttg tgatccgccg
     8761 ctgacaacca tcgcgcagcc gcgttacgaa atcggtcggg aagctatgct gttattgctt
     8821 gatcaaatgc aggggcaaca cgttggcagt ggctctcgtt taatggactg cgaacttatc
     8881 atccggggat caacacgcgc gttaccttaa agtaaacctt taagactgcc gtgactggtc
     8941 aaaggcccgc cgcttaagta acatggcggg ctgacgaacg aataaataca gcgaaacgat
     9001 agtggcacaa cgagattatg tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag
     9061 cacctcacgg aaaaagcaac gaaatctgcc tgcggtttct cccgctatgg tcgctattgc
     9121 tgccgccgtt cttgtgacct ttatcggtgg tctgtacttc attacgcatc acaagaaaga
     9181 agagtccgag acgctgcaaa gccagaaagt gaccggaaac ggactaccac caaaaccaga
     9241 agaacgctgg cgctacatca aagagctgga aagtcgccag ccgggtgtgc gtgcgcctac
     9301 agaaccttct gccggtggtg aagtgaaaac gccggagcaa ctgacaccag aacaacgcca
     9361 gcttcttgag caaatgcagg ctgatatgcg ccagcagcca acgcagctgg ttgaagttcc
     9421 gtggaatgaa cagacgccgg aacagcgtca gcaaacgcta cagcgccagc gtcaggcgca
     9481 gcaattagcg gagcagcaac gcctggttca gcagtcgcgt acgactgaac aaagctggca
     9541 gcagcagacg cgtacgtcgc aagccgcgcc ggtacaggcg caaccgcgcc agtctaaacc
     9601 ggcttctact cagcagccgt accaggatct gctgcaaacc cctgcgcaca cgaccgcgca
     9661 gtcgaagccg cagcaagctg cgccggttgc tcgtgtcgct gacgcgccaa aaccgacggc
     9721 ggagaaaaaa gacgaacgcc gctggatggt gcagtgcggt tcgttcagag gcgcggaaca
     9781 ggcagagacg gtacgtgctc agctggcgtt cgaaggcttt gactcaaaaa ttaccaccaa
     9841 caatggctgg aatcgtgtgg tcattggccc ggtgaaaggc aaagagaacg cagacagcac
     9901 cctcaatcgg ttgaagatgg cgggtcatac aaactgcatt cggctcgccg ccgggggttg
     9961 aaaccctcaa aatccccccc atctataatt gcattatgcc ccgtactttt gtacggggtt
    10021 tgtactctgt attcgtaacc aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt
    10081 aacggccatg tggtcatcgc tggtgatggt caggccacgt tgggcaatac cgtaatgaaa
    10141 ggcaacgtga aaaaggtccg ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc
    10201 ggtactgcgg atgcttttac gctgttcgaa ctgtttgaac gtaaactgga aatgcatcag
    10261 ggccatctgg tcaaagccgc cgttgagctg gcgaaagact ggcgtaccga tcgcatgctg
    10321 cgcaaacttg aagcattgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt
    10381 aacggtgacg tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac
    10441 gcccaggctg cggcgcgcgc gctgttagaa aacactgaac ttagcgcccg tgaaattgct
    10501 gaaaaggcgt tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc
    10561 gaagaattaa gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc
    10621 gtcagcgaac tggataagca catcatcggc caggacaacg ccaagcgttc tgtggcgatt
    10681 gctctgcgta accgctggcg tcgcatgcag ctcaacgaag agctacgcca tgaagtgacc
    10741 ccgaaaaata tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt
    10801 ctggctaagc tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt caccgaagtg
    10861 ggctatgtcg gcaaggaagt ggattctatt attcgcgatc tgaccgatgc cgccgtgaaa
    10921 atggtacgcg tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa
    10981 cgtattctcg acgtgctgat cccacctgct aaaaacaact ggggacagat cgaacagcag
    11041 caggaaccgt ccgctgctcg tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat
    11101 gacaaagaaa tcgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct
    11161 ccgggcatgg aagagatgac cagccagctg cagtccatgt tccagaacct gggcggtcag
    11221 aagcaaaaag cgcgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa
    11281 gcggcgaaac tggtgaaccc ggaagagctg aagcaagatg ctatcgacgc tgttgagcag
    11341 cacgggatcg tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt
    11401 ccggatgttt ctcgtgaagg cgttcagcgt gacctgctgc cgctggtaga aggttgcact
    11461 gtttccacca aacacgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg
    11521 ttccagattg cgaaaccgtc tgacctgatc ccggaactgc aaggtcgtct gccaatccgc
    11581 gttgagctgc aggcactgac caccagcgac ttcgagcgta ttctgaccga gccgaatgcc
    11641 tctatcactg tgcagtacaa agcactgatg gcaactgaag gcgtaaatat cgagtttacc
    11701 gactccggta ttaagcgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac
    11761 atcggtgctc gtcgtttaca taccgttctg gagcgtttaa tggaagagat ttcctacgac
    11821 gccagcgatt taagcggtca aactatcact attgacgcag attatgtgag caaacatctg
    11881 gatgcgttgg tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt
    11941 ttcatcattg tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa
    12001 caacaaatta gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc
    12061 gcctttgctg caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatcca
    12121 ctggtcgccc tgctggcatt gattaccgcc gggctattac agatcctttc taacctcgcc
    12181 aatgattacg gcgatgcggt aaaaggcagc gataaacctg accgcattgg gccgctacgc
    12241 ggcatgcaaa aaggggtcat tacccagcaa gagatgaaac gggcgctcat tattaccatt
    12301 gtgctcatct gtctttccgg gctggcactg gttgcagtgg cgtgccatac gctggccgat
    12361 tttgtcggtt tcctgattct tggtgggttg tcgatcattg ccgctatcac ctacaccgtg
    12421 ggcaatcgtc cttatggtta tatcggtttg ggtgatattt ccgtactggt tttctttggc
    12481 tggttgagcg tcatggggag ctggtattta caggctcata cattgattcc ggcactgatc
    12541 cttccggcaa ccgcatgcgg cctgctggca acggcagtac tgaacattaa taacctgcgt
    12601 gatatcaata gcgaccgcga aaacggcaaa aacacgctgg tggtgcgctt aggcgcagta
    12661 aacgcgcgtc gttatcatgc ctgcctgctg atgggctctc tggtgtgtct ggcgctgttt
    12721 aatctctttt cgctgcatag cctgtggggc tggctgttcc tgctggcggc accgttgctg
    12781 gtgaagcaag ctcgttatgt gatgcgcgaa atggacccgg tggcgatgcg accaatgctg
    12841 gaacgcactg tcaaaggagc gttactgact aacctgctgt ttgttttagg gatattccta
    12901 agccagtggg cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc
    12961 cacatagcgc gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg
    13021 atacttccga gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct
    13081 ccaactttgg cggacgagcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg
    13141 acaacgggtt gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg
    13201 atggcggagg ttctgttcgt cgcgcactgg tcgatgctga actggcgcgt ctggcagtac
    13261 aaaatgaatg ggaaggtctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag
    13321 agttggatat cggcatccag gcgatggcgg caattccggt tggtgccgct ggcgaaggca
    13381 ttggcgaaag cgatgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc
    13441 tttatgccga caataccggg attattcttt cagaagatcc gctggatatt gaatgataaa
    13501 aaaggcaccg caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt
    13561 acgcgaaaga tgaagtgtaa tcagacctct tccatgcgac ccagcagggc ctgtagacgt
    13621 tcctgccagc cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc
    13681 tgatgctggg cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg
    13741 atttccatct gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc
    13801 tcaaacactt ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa
    13861 aatcctcgtc ccgattaccg gtgacgcctt aataaatacg agcgcacttt agttagctcc
    13921 gattgtatga agccgcgcca tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca
    13981 cacttttcat ccttctcctg gtgacataat ccacaccaat cgaaaatgtt aataaatttg
    14041 ttgcgcgaat gatctaacaa acatgcatca tgtacaatca gatggaataa atggcgcgat
    14101 aacgctcatt ttatgacgaa gcacacacat tttaagttcg atatttctcg tttttgctcg
    14161 ttaacgataa gtttacagca tgcctacaag catcgtggag gtccgtgact ttcacgcata
    14221 caacaaacat taactcttca ggatccgatt atgagtcaaa catcaacctt gaaaggccag
    14281 tgcattgctg aattcctcgg taccgggttg ttgatttttt tcggtgtggg ttgcgttgca
    14341 gcactaaaag tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat ttggggactg
    14401 ggggtggcaa tggccatcta cctgaccgca ggggtttccg gcgcgcatct taatcccgct
    14461 gttaccattg cattgtggct gtttgcctgt ttcgacaagc gcaaagttat tccttttatc
    14521 gtttcacaag ttgccggcgc tttctgtgct gcggctttag tttacgggct ttactacaat
    14581 ttatttttcg acttcgagca gactcatcac attgttcgcg gcagcgttga aagtgttgat
    14641 ctggctggca ctttctctac ttaccctaat cctcatatca attttgtgca ggctttcgca
    14701 gttgagatgg tgattaccgc tattctgatg gggctgatcc tggcgttaac ggacgatggc
    14761 aacggtgtac cacgcggccc tttggctccc ttgctgattg gtctactgat tgcggtcatt
    14821 ggcgcatcta tgggcccatt gacgggcttt gccatgaacc cagcgcgtga cttcggtccg
    14881 aaagtctttg cctggctggc gggctggggc aatgtcgcct ttaccggcgg cagagacatt
    14941 ccttacttcc tggtgccgct ttttggccct atcgttggcg cgattgtagg tgcatttgcc
    15001 tatcgcaaac tgattggtcg ccatttgcct tgcgatatct gtgttgtgga agaaaaggag
    15061 accacaactc cttcagaaca aaaagcttcg ctgtaatatg actacgggac aattaaacat
    15121 gactgaaaaa aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt
    15181 aatggatcac gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc
    15241 aaaaccaggc tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct
    15301 ggtagaagtg ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac
    15361 gaaccagcgt gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc
    15421 cattgtctgg cagtgccgtc gtaccgccga gatctgcgaa catctcaaac gcgatggcat
    15481 ggaagagtac attcgtaaca acaccggtct ggtgattgac ccgtacttct ctggcaccaa
    15541 agtgaagtgg atcctcgacc atgtggaagg ctctcgcgag cgtgcgcgtc gtggtgaatt
    15601 gctgtttggt acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt
    15661 gaccgattac accaacgcct ctcgtaccat gttgtttaac atccatactc tggactggga
    15721 cgacaaaatg ctggaggtgc tggatattcc acgcgagatg ctgccagaag tgcgtcgttc
    15781 ttccgaagta tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc
    15841 cgggatcgcc ggtgaccagc aggcggcgct gtttggtcag ttgtgcgtga aagaaggaat
    15901 ggcgaagaac acctatggca ctggctgctt tatgctgatg aacactggcg agaaagcggt
    15961 gaaatcagaa aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtaaacta
    16021 tgcgctggaa ggtgcggtgt ttatggcggg cgcgtccatt cagtggctgc gcgatgagat
    16081 gaagttgatt aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa
    16141 tggtgtgtat gtggttccgg cctttaccgg gctgggtgcg ccatactggg atccgtatgc
    16201 acgcggggcg attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac
    16261 gctggagtct attgcttatc agacgcgtga cgtgctggaa gcaatgcagg ccgactccgg
    16321 tattcgtctg cacgcgctgc gcgtggacgg cggtgcggtg gcgaacaatt tcctgatgca
    16381 gttccagtcc gatattctcg gtacgcgcgt tgagcgcccg gaagtgcgcg aagtcaccgc
    16441 tttgggtgcg gcctatcttg ctggtctggc ggttggcttc tggcagaatc ttgacgagct
    16501 gcaagagaaa gcggtgattg aacgcgagtt ccgtcctggc atcgaaacca ctgagcgtaa
    16561 ttaccgttac gcaggctgga aaaaagcggt caaacgcgcg atggcatggg aagaacacga
    16621 cgagtaataa tgattgcctg atgcgacgct tg
//
LOCUS       DADTFG010000111        16623 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.112, whole genome shotgun sequence.
ACCESSION   DADTFG010000111 DADTFG010000000
VERSION     DADTFG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.112"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            384..590
                     /locus_tag="J1138_003319"
     CDS             384..590
                     /locus_tag="J1138_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455081.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1143..1682
                     /locus_tag="J1138_003320"
     CDS             1143..1682
                     /locus_tag="J1138_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HBA8455082.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATVRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIQVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            1691..3790
                     /locus_tag="J1138_003321"
     CDS             1691..3790
                     /locus_tag="J1138_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA8455083.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WEPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPSPEPVPTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            3787..3999
                     /locus_tag="J1138_003322"
     CDS             3787..3999
                     /locus_tag="J1138_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455084.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3999..5507
                     /locus_tag="J1138_003323"
     CDS             3999..5507
                     /locus_tag="J1138_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA8455085.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQMRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5452..7479
                     /locus_tag="J1138_003324"
     CDS             5452..7479
                     /locus_tag="J1138_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBA8455086.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGMAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGNAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7566..7889
                     /locus_tag="J1138_003325"
     CDS             7566..7889
                     /locus_tag="J1138_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBA8455087.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7882..8157
                     /locus_tag="J1138_003326"
     CDS             7882..8157
                     /locus_tag="J1138_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HBA8455088.1"
                     /translation="MSDPFSRLVARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8169..8759
                     /locus_tag="J1138_003327"
     CDS             8169..8759
                     /locus_tag="J1138_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455089.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRRLPEARFATGHDHQGRPRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8756..9157
                     /locus_tag="J1138_003328"
     CDS             8756..9157
                     /locus_tag="J1138_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455090.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9168..9911
                     /locus_tag="J1138_003329"
     CDS             9168..9911
                     /locus_tag="J1138_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455091.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVAPASVSVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            9972..10358
                     /locus_tag="J1138_003330"
     CDS             9972..10358
                     /locus_tag="J1138_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA8455092.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDDEHEALTPEKS"
     gene            10367..10696
                     /locus_tag="J1138_003331"
     CDS             10367..10696
                     /locus_tag="J1138_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA8455093.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10668..13724
                     /locus_tag="J1138_003332"
     CDS             10668..13724
                     /locus_tag="J1138_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA8455094.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTLVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSISDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATDRMTEAHKQLLALQQRISDLDGKKLTANEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEIAQGDWKNGVLRALQNVTENADNTAGTVEQLFTSAFSSMSDWLATFCTT
                     GKLNFKSFTSSVLSDMSRIMAQIALMKAVKGIASALPFDFVANADGGVYQSADLSRYS
                     GTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQYNI
                     EINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13724..14053
                     /locus_tag="J1138_003333"
     CDS             13724..14053
                     /locus_tag="J1138_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455095.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            14063..14761
                     /locus_tag="J1138_003334"
     CDS             14063..14761
                     /locus_tag="J1138_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA8455096.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14766..15509
                     /locus_tag="J1138_003335"
     CDS             14766..15509
                     /locus_tag="J1138_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455097.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FSCVPHLTGRRFEHGATDCYTLFRDAYHLAGIEMPDFHREDDWWCNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            15407..16054
                     /locus_tag="J1138_003336"
     CDS             15407..16054
                     /locus_tag="J1138_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBA8455098.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     STQLPAFRQKLNDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTATSTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16115..>16623
                     /locus_tag="J1138_003337"
     CDS             16115..>16623
                     /locus_tag="J1138_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA8455099.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVG"
BASE COUNT         3996 a         3849 c         5006 g         3772 t
ORIGIN      
        1 tttagctcag aataaaataa ttcacagttt aaatctttac gcacttgatc gaatatttct
       61 ttaaaaatgg cagcctgagc cattggtaaa ccttccatgt gatacgatgg cgcgtagttt
      121 gcattatcgt gtttattgtt tcaatctggt ctgacctctt tgtgttttgt tgatgattta
      181 tgtcaaatat taagcctgtt tttaatgaat agtgttgatt gcgtaacaaa gttgagcctt
      241 gctggcatcc aggagggata tgcaaccgac agatgtatgt aaggtcgatg tactcaaact
      301 ttcatacttt tcctctttta tgcagaaaga tttgaagtaa tattttaacc gctagatgac
      361 gagcaaacgc atggagcgac aaaatgaata aagaacaatc tgctgatgaa ctctcgttgg
      421 atctgattcg tgtaaaaaat atgcttaata gcaccatttc tatgagttac ccggatgttg
      481 taattgcatg tatagaacat caggtgtctc tggaagcatt cagggcaatt gaggcagcgt
      541 tggtgaagca cgataagaat tcgaaggatt attccctggt ggttgactga gcaccataac
      601 tgctaatcat tcaaactatt tcacctgtga cagagtcaat atcgcattct gtcactgtca
      661 ggctaataca gagctgcaat tcaactactg caatgtcctc gtaattaggt gaatttacaa
      721 tatcgtcctg ttcggatgcc ggctgcattg ctgaagatga ggcatttatg gttcgcatat
      781 tttcccctca tgctcgtcag tcctgtgcgt aggaagaaac aggacactca cactaatttg
      841 tgtgggcatg ctgtgatgtc cttctgaatt attcctatgc cattatgtaa agcgctgtat
      901 cagatgctcg tcacggctgt caggctgtcg ggtcctcccg gtgggggccc ctgccacggg
      961 gcgggagcgt cgcggaaaaa gactagtttt tgcatttcca tggcggcggc agcatgtttg
     1021 gtaatttatt gataattaaa agttatttct cttttcacct gtacaatatt tttttctccc
     1081 tgtcattaga ccagtttgca attaattgaa atatataaat aaacctgatt ttcacctgcc
     1141 agatggagtt gcttatgtca aatgtgagcg ggatcggtga tgcttattac tggagtgttt
     1201 ttaaaatcgc cgaggccttt gggcttcacc gggacacagt aaaaaaacgg ctcctcgcgg
     1261 ccaacactcc tgtggctgcg actgtcaggg ggaaccccgt ttacgccctg cagcatgtcg
     1321 ggcccgccct gtttagtgtg aagcatgagg cagcagactc tgttcatgat ccatcccgta
     1381 tggagccgaa agagagaaag gactggtacc agtctgaaaa tgaaaggatc aagctggaaa
     1441 aggagcagcg aaaactcatc caagttgatg aagtagtcat cgtctattcg tccatgagaa
     1501 aggctgtcgt ccaggttctg gagacaattc cggatgttct tgaacgcgat tgcgccctga
     1561 ctcctcaggc cgtcggcgtt gtacagcagg ccattgatga cctgcgatac actcttcagg
     1621 aaaaatccta cgaggcttgt gctgctgaat taattcctga tgaggaagga gagagtctct
     1681 aggaggaatt atgggttttt catcagcccg aaatttggga agggacatat cggcaggatt
     1741 ttccccacca cgtcgcatgc cgatttcgga ggctgttaaa aaattcatgc gtgttcccaa
     1801 gggggctggt aactcggtgc catgggagcc tgaactgaca ccctacatca ttgagcccat
     1861 gaactgcctg gcatcgcgtg aatacgatgc ggtgattttt gttggtcctg cgcgaacagg
     1921 gaagaccatt ggtctgatcg atggatggat tgtctatacc atcgtttgcg atccttcgga
     1981 catgctcgtt gtgcagatga ccgaagataa ggcccgcgag cattctaaaa agcgcctcga
     2041 cagaacgttc agaagcagtg cggcggtaaa gaaaagaatg agtccacgtc gtaacgacaa
     2101 taatgtccat gataagacgt tcagggatgg ctcgttcctt aaaattggtt ggccctcggt
     2161 caacattatg tcgtcgtcgg attaccggtt tgtcgcctta accgattacg accgttttcc
     2221 ggagaatatc gacagcgagg gtgatggttt ctccctggcc tcaaaacgta ccaccacatt
     2281 tatgtccgcc gggatgactc tggtggagag ctcgccggga cgtgacatct gcgacagcaa
     2341 atggcgacgt aagtcgcctc atgaagcgcc accgacgact ggtattcttt ccctttacaa
     2401 tcgtggtgac cgccgccgct ggtactggcc atgtccgcac tgcggtgaat attttcagcc
     2461 agctatggat gccatgaccg gctaccgtaa tgaaccggat ccctttaaag ctagtgaggc
     2521 ggcgtatcta ctttgcccgc actgcagcgg cattatcact gcggagaaaa agcgtgagct
     2581 caatagtgca ggagtctggt tgcgtgaagg tcaggtcatt gatcgtaacg gcaacgtttc
     2641 cggtgaaccg cgccgctccc gtatcgccag tttctggatg gaagggccag ctgctgcgta
     2701 tcagacctgg gcgcaactgg tttacaaatt actgactgca gaacaggagt atgaagcgac
     2761 aggaagcgaa gaaacactca gggcggttat caacaccgac tggggattgc cttatcttcc
     2821 tcgtgccagc atggagcaac gaaaaagtga actgcttgag cagcgggcag agccagttcc
     2881 ttcccgcagt gttccggatg gcgttaattt ccttgtggcg acagtggatg tgcaggcggg
     2941 acgtcatcgc cgttttgtgg ttcaggtaac gggctatggc agccgtggcg aacgctggat
     3001 tattgatcgt tacaacatca cgcagtcatt gcgcagtgac tgcgacgggg agagccagcg
     3061 aattgatccg gccagctatc cggaagactg ggatgtcctg ctgacggatg tttttcataa
     3121 aagctggccg ctggcctccg atccttctca acaaatgcga ctgatggcaa tggcggtgga
     3181 ctccggcggt gaagacgggg tcactgataa tgcctataaa ttctggcgtc gttgccgtcg
     3241 tgatggcctt ggtaaacgta tttacctgtt taagggcgac agcatccggc gcgcaaaact
     3301 gatcagccgt acattccctg ataacaccgg acgaacgggc cgccgggcgc aggccgcagg
     3361 tgatgtgccg ctctggcttc ttcagacgga tgccctgaaa gaccgggtga ataacgcgtt
     3421 atggcgtgac tcgccaggtc caggctatgt gcatttccct gactggctgg ggagctggtt
     3481 ttacgacgaa ctgacgtatg aagagcggag cagtgacggg aaatggagta agccgggtcg
     3541 cggtgccaac gaagcttttg acctgatggt gtatgccgag gcgctggtca ttctgcatgg
     3601 atacgaaaag atccgctggc cggatgcacc ggagtgggcg agccgggaaa cctggctgga
     3661 gtgtgtcccg gacagtaccg aaccgtcacc ctcaccggaa ccggtaccca cgcctgttaa
     3721 aaaacaaaaa cggaagaaaa cagtaactga cgatgttaac ccctggctga cttccggagg
     3781 atggttatga atcagaatga tattgaagcc atgattcagc gttatacgga agctgaaatg
     3841 gcggtgctgg acggaaaatc cgtcactttt aatggtcagc agatgaccat ggaaaactta
     3901 tctgagatcc ggcagggacg gcaggagtgg gagcgccgcc ttgcggctct gattacacga
     3961 cgacgggggc atcccgggta ccggctggcg aggttctgat ggcaattctt gatgatgtga
     4021 ttggcgtttt ttcaccagga tggaaagcgg caaggctgcg ttcccgtgcg gtgatccagg
     4081 cttatgaggc cgtaaaaacg acgcggacac acaaagcccg gcgggaaaac cgaactgccg
     4141 accagttaag ccagtacggg gccgtgtcgt tacgtgagca ggcccgttac cttgataaca
     4201 accacgatct ggttattggt gtatttgaca agctggaaga acgggtggtg gggaaaaacg
     4261 ggattattgt cgagccacat ccggtattac gcaatggggc cattgcccgt gatctggcag
     4321 cggagattcg cacccgatgg agtgaatggt ctgtcagccc ggaagtcacc gggcagttta
     4381 cccgtccgat gctggaacgt ctgatgctgc gtacctggct gcgcgatggt gaggtgtttg
     4441 cccagatggt ttccgggcgc ataaacagcc tgacgccttc tgccggtgtt catttctggc
     4501 tggaggcgct cgagccagac tttattccca tgaccagtga tgagagcaac aggctgaatc
     4561 agggcgtgtt tgttgatgac tgggggcgtc ccgaaaaata tctggtgtat aaaagccgtc
     4621 ccgtatccgg acggcagatg gaaaccaaag aagtggatgc agagcgaatg ctgcatctta
     4681 aatttgttcg ccgtctgcac cagatgcgcg ggacgtcttt gttgtccggt gtgctgatcc
     4741 gcctcagtgc cctgaaagag tatgaagatt ctgagctgac tgcagcaagg atcgccgctg
     4801 ctctggggat gtacatccgc aaaggcgacg ggcagagcta tgaaccggat ggtaatggca
     4861 gcaaggataa ggaacgcgag cttaccattc agccaggcat tatttacgac gatctgaaac
     4921 ccggcgaaga aattggaatg gtgaagtcgg atcgccccaa tcctaatctt gaaacttttc
     4981 gtaatggtca gttgcgtgca gtggcggctg gcagtcgtct gagtttttcc agtacagcgc
     5041 gcaactataa cggcacttac agcgcccagc gtcaggagct ggttgagtct actgatggct
     5101 acctgatcct gcaggactgg tttattggtg ccgtcacccg cccgatgtat cgtgcatggc
     5161 tgaaacaggc tgtggcatcc ggtgttatca ggttaccccg cgatcttgac cgttcttcac
     5221 tgtataccgc ggtgtattcc ggaccagtga tgccgtggat tgaccctgtt aaggaggctg
     5281 aggcctggaa aatccagatg cgtggtggag cggcgacaga atcagactgg gtacgtgctg
     5341 gtggtcgtaa tccggatgat gtcaaacgtc ggcgcaaggc cgaaattgat gaaaaccgca
     5401 agctggatct ggtatttgat accgatccgg ccagtgataa aggaggcagc agtgccgcaa
     5461 cgaaacgaca ggagccgcag cacaccgacg accagtccga agaataattc ctggttcagg
     5521 atgcaggctg gtcaccagag tgacgcggat atttatattt atgacgagat tggtttctgg
     5581 ggtgttacag cgaagcagtt tatcagtgat ctgaatgcac tgggcgatat cacccacatt
     5641 aatctccata ttaattcacc gggtggcgat gtctttgaag gcatcgccat ttttaatgcg
     5701 ctgaaaacac atggtgcgtc cattaccgtt tatgtcgacg gtatggcggc gtcaatggcg
     5761 tcggtcattg cgatggtggg aaacccggtc attatgccgg aaaacacctt catgatgatt
     5821 cataaaccat ttggctttac gggcggtaat gcggaggaca tgcgcaccta tgccgacctg
     5881 ctcgataaag ttgaggcggt tctgttaccc gcttatgcac agaaaaccgg gaaaaccacc
     5941 gatgaaattg ctgccatgct ggcggatgag acctggatgt ccggtgccga atgtctggca
     6001 catggatttg ctgatcaggt aacgccagcc gttaaggcaa tggcatgtat tcagtcaaaa
     6061 cgtacagagg aatttaaaaa gatgccggaa tccattcgaa acatgattac tccgccacgc
     6121 aacagtgctc cacgcgtaca ggatgatgaa cctgcagcct cccggacgcc agtgcaggca
     6181 gcagcacccg tggtggatga aaacagcatc cgtgcgcagg tcctggcaga gcaaaaagcg
     6241 cgtgtaaacg gtattaatga tctgtttgcc atgtttggcg ggcgttatca gtcgctgcag
     6301 gctcagtgtc ttgccgatcc tgaatgttcg ctggagcagg cccgcgaaaa gctgttgaac
     6361 gagatggggc gcgagtccac gccatccaat aaaaataccc cggctcatat ttatgccggt
     6421 aacggtaatt ttgtggggga cgggatccgc caggcgctga tggcgcgtgc cggatttgaa
     6481 aaaaccgaac gtgataatgt ctacaacggg atgaccctgc gtgaatatgc ccgtatgtca
     6541 ctgactgaac ggggtattgg ggtttccagt tataacccga tgcagatggt cggtgcggcg
     6601 ttcacacaca gtacgtctga cttcggtaat attctgctgg atgttgcgaa caaagccatt
     6661 ctgcagggct gggaagatgc ccctgaaacc tatgaacagt ggacgcggaa aggtcagttg
     6721 tctgatttta aaattgccca tcgtgtgggt atggggggct tcagtgctct gcgtcaggtg
     6781 cgtgaagggg cggaatataa atacgtcacc accggagata aacaggccac tattgcactg
     6841 gcgacctatg gcgagctgtt cagtatcacc cgtcaggcca ttatcaatga tgatctgaat
     6901 atgctgaccg atgtcccgat gaaactgggc cgtgcggcga aatccactat tgccgatctg
     6961 gtttatgcca ttctgacgtc taacccgaaa atctccacag ataatgtaag tctgttcgat
     7021 aaagcgaaac atgcaaacgt actggagagc gctgcaatgg acgtggcatc gctggataaa
     7081 gcccgccagt tgatgcgcgt tcagaaagag ggggagcgtc atctgaatat tcgtcctgcg
     7141 ttcgtactgg taccgacggc gatggagtct gttgctaacc aggtcattcg ctcctcaagt
     7201 gtcaaggggg ctgacattaa cgccggtatt attaacccgg tgaaagattt tgcgaccgtt
     7261 attgcagagc ctcgtcttga tgataacagc cagaccacct tctacctggc tgcgtcaaaa
     7321 ggctccgata cgattgaagt ggcttatctc aacggtgtgg atacgccata tattgatcag
     7381 atggagggct tcagtgtgga tggcgtgaca acgaaagtgc gtattgacgc cggtgtcgcg
     7441 ccagttgatc accgcggtct ggtgaaatgt acggcgtaaa cgtcgcagac aacaactctg
     7501 atggcccgta agggcttttt ttgtacctga aatcagcccc tgaatggggc tgtgcggaga
     7561 cagttatggc aaagaatttt gtagaagaag gaaaaacggt ggcgattgtt gccagtgcag
     7621 ccatcagcag cggagatctg gtgcaggtgg gtgatgtttt tgcggtggcg ctgaccgata
     7681 ttccacaggg tgaaacaggc gacggcatga ccgaaggtgt gtttatgctg cctaagctga
     7741 aaacggatga catgaaaacg ggtaagaagg tttatctgaa gtccggaaaa gttcagctga
     7801 ctaacagcgg ctctgatccg ctggtcgggg ttgtctgggc agatgccgga accagtgcag
     7861 aagaagtgcc ggtaaaactc aatgtctgat cccttttccc ggctggtagc gcgtatggat
     7921 gcgatcacgg tcagaaagat gggaaagaca gcctcgatta atgatgtcga tatgactgtg
     7981 atcccgggag aaacactggc agagctgaat gctctgtccg gacctgcggt ctctctggtg
     8041 gtgttttctt cgggataccg cccacggcgc ggggatcgcg ttgtttatga cggacaacaa
     8101 tggacggtca cacggcatga acgctttaac ggtaagccaa tgatctttat tgagtaaaga
     8161 ggtatgggat gaaggggctt gagaatgcca tccgcaatct gaacagcctt gatacccgta
     8221 tggtgccaca ggccagcgca tgggcgataa accgtgtggc acagaaagcg gtctcggttg
     8281 ccacccggca ggttgccggg aataccgttg cgggagataa ccaggtgaaa gggatccccc
     8341 tgaaactggt acgtcagcgt gtccgggtgt ttaaagccag tccgtcagga aaaatgacgg
     8401 ccaggatccg cgttaaccgg ggcaatctgc ccgccatcaa actgaacaca acacggcggc
     8461 gtgctggtga aggactgaga gtgggaaaat actttttccg gggggcattt gttcagcaac
     8521 tggcgaatgg ccgctggcat gttctgaggc gtcttcctga agcgcgtttt gcaacagggc
     8581 atgaccatca gggcagacca agaaaaaatc gtcttcctgt ggaggtagtg aaaatcccgc
     8641 tgtccggacc gctgacacag gcatttgaag atgcccgcga ccgcatcatt gctgcggaaa
     8701 tgccgaaaca gctggggtat gcactgaaac aacaactgag gttatggctg acccgatgaa
     8761 ccgacataca caaatccgcc aggtcgtact ggcacgcctt cgggaacagt gtggagacag
     8821 cgccacgttt tttgacgggc ttccggcatt tgttgatgcg caggaactgc ctgccgtggc
     8881 ggtgtggctg agtgatgctc agtacaccgg aaaaatgacg gatgaagatg actggcaggc
     8941 tgttctgcat attgctgtct tcatccgggc acaggcaccg gattcagagc tggatatgtg
     9001 gatggagagc accattttcc cggccctgaa tgatgtaccg gcactttccg gactcatcga
     9061 caccctgatc ccactcggtt ttaactatca acgtgataat gagatggcca cctgggcgat
     9121 ggcggaaatc acgtaccaga tcacgtacac gaattaagga ggtggcaatg accacaccaa
     9181 atccactggc aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg
     9241 acgcatttgc gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc
     9301 tgcaaccggg cgaaatgacc gctgatgcag aagatgatac ttatctcgat gatgaagatg
     9361 cagactggaa aacgacaacc caggggcaga aatcagtcgg tgatacttcg gcgacgctgg
     9421 cctggcgtcc gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag
     9481 tctgcgcgtt tcgtatcaaa tatcccaacg gtactgttga tgttttccgt ggctggctga
     9541 gttcactggg taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg
     9601 gtgtggggcg tccgtatctg gcagaggaag gcactgaaac agtgagcgtt accgggctga
     9661 cggtggcacc ggcatctgtc agtgtaaaag tgggagcaac caccacgctg acctttacag
     9721 taaaacctga cggagccagt gacaaagcga tcagtgtgca ttcgtcagat ccacagactg
     9781 ccacggtgac cctgaacggg cttgtagcca cggtgaaagg cgtgaagcag ggcagtgtca
     9841 gcattgtggg catgaccgct gacgggaatt ttgtggctgt ggctgcggtg actgtcagcg
     9901 ccgcaggtta acaggacgat actcatcatt tgccccggtt atccggggct tttttgcagg
     9961 tggagaacat gatgtttctg aaacagggca cgtttaatta tgaaaaacag tccgtggtgc
    10021 tcagtgagct gtccgggctg cagagaattg aatatctggc gtttgttcag cagcgaacgg
    10081 caaagtttga tgccggtgag ggagaactgc cggaggctga acgacagatt gcttttctgc
    10141 ggatggggat ggatatcaat gcctggctgg tttcccgctc actgtggaat gcggatcagt
    10201 ctaaggatgt agagacgctt tgcgcatccg ttattacaac atggtcgtat gatgccctgg
    10261 gtgcgggggc ggagatggtt ctgtcgctga gcggtatggg ggccattgat aatgccgggg
    10321 atgatgagca tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat
    10381 gcggcttgcc cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg aaatgtcggc
    10441 cactgagctt ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat
    10501 ggatgcgcag tttgcctcgc tgaaggcatt gatcgtgaga atggtatccg gcagcagtga
    10561 tgctgcggtg gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga
    10621 cgaagaactg atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag
    10681 ccaacctggt cattgatttg gggattgatg cggcagagtt taaaaatgaa atcccccgta
    10741 tcaaaaacct tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc
    10801 gttttatgga gcgtcagact caggccgccc ggcagacaac gcaggcggcg tcttcggctg
    10861 caacagccgc atccgtccat gcgcagacag tggagaagaa cgcacaggct catgaacgca
    10921 tggcccgcga ggtggagaaa acccgccagc gcatggaggc gctgagccag aaaatgcgcg
    10981 aggaacaggc gcaggccatg gctctggcgg aggctcagga taaagcggct gctgcgtttt
    11041 atcgtcagat tgacagtgtg aaacaggcca gtgcggggct gcaggaatta cagcgtattc
    11101 agcagcagat ccgacaggcc agaaacagtg gcgggattgg tcagcaggat tatctggcgc
    11161 tgatttctga ggttacggcg aaaacccttg ttcttacaca ggctgaggaa gaggctaccc
    11221 gacagaaagt ggcgtttatc cgtcagctta aagagcaggc aacccgccag aatctttctt
    11281 cttctgagtt gcttcgtgct aaggctgccc agctgggggt aagcagtgct gcagaagtgt
    11341 atatccgcaa aatggagcag gcaggaaaag ccacgcattc gctgggtctg aaaagtgcag
    11401 cggcccgtca ggagataggc gttctgatag gtgaactggc ccgcggaaat ttaggtgcgc
    11461 tgaggggatc cggaataacg ctggctaacc gtgccgggtg gatagacaca ttgatgtcac
    11521 cgaaaggcat gatgcttggc ggggttattg gcggtattgc cgcggctgtc tatggtctgg
    11581 gtaaagcctg gtatgacggt cagaaggagg gggaagaatt taaccgccag ctgtcgctga
    11641 cggggcatta tgccggagtc actgccgggc agctgtggac gctcagtcgt gctatttccg
    11701 ggaatggtat cacgcaacat gctgcagccg gtgcgctggc tcaggtggtg gggagtggtg
    11761 catttcgtgg aaacgatatc ggtatggtgg cgagagctgc cgcacagatg gagcgatcgg
    11821 ttggccagtc gatcagcgat accataaatc agtttaagcg gctgaaggat gatcctgtaa
    11881 atgccgcgaa ggctctggac aatgagctgc attttcttac tgccactcag cttgagcaga
    11941 tacgtgtcct tggggatcag ggacggtcca gtgatgctgc acggatagcc atgtctgcac
    12001 tggcagagga aaccggtcgg cgtactgcgg atattgataa taacctcaat gcgcttggta
    12061 gtacgctgaa gtatctgtct gatttgtgga gtcgtttctg ggatgcggcc atgaatattg
    12121 gtcgtgaaga ctcgctggat gaacaaattg ccgctttaca ggagaaagtg tcgcgggcga
    12181 aaagactccc ctggacggca tcatcttctc aggttgaata cgatcagcag cgtcttaacg
    12241 atcttcagga gaaaaaacgc cagaaggatt tgcaggatgc aaaagagcag gcagagcgga
    12301 attatcagga gcaacagaaa cgccgtaatg ctgaaaatgc tgcactgaac cggatgaatg
    12361 aaactgaagc agcacggcat cagcgtgaaa ttgcgcgtat taatgccatg cagtacgccg
    12421 atcaggctgt cagggatgcg gcgatacaac gtgaaaatga acgttacgag aaagccctgg
    12481 catccggtaa gaaaaaaaca cgcgaaaccc gtaatgatga ggccacccgg ttattgctgc
    12541 agtacagtca gcaacaggca caggtggaag ggcagattgc tgctgcaaga cagtcagcag
    12601 gcattgccac tgacaggatg acagaagcgc ataaacagct tctggctctg cagcagcgca
    12661 tcagcgacct ggacgggaaa aaactgacgg caaatgaaaa gagtgtgctt gcccgtaaag
    12721 atgaactgat tcaggcattg acgctgctgg atgtaaaaca gcaggagctt cagaaacaga
    12781 cggcactcaa cgagctgaag aaaaaaacaa ttcagctgac cagtcaactg gctgaagaag
    12841 agcgcgctca gcgtcagcaa catgacctgg atatcgccac ggtgggtatg ggtgatcagc
    12901 agcggcagcg atatcaggta caactgagtc ttcgccagaa ataccagcaa cagctggagc
    12961 agttgaggcg ggatagtgag cagaaaggga catataacac ggatgactac agaaaggccg
    13021 agcaggcgct gacggagagc ctgaaccgac aactgaatga gaatcgccgt tactggcaac
    13081 agcttgaaat tgctcagggt gactggaaaa acggagtcct gcgtgcactc cagaatgtca
    13141 ctgagaatgc ggataacaca gccgggacag tggaacagtt gttcacgtct gcgttcagta
    13201 gcatgagtga ctggctggcg acattctgta ctacaggcaa actcaatttc aaatccttca
    13261 cctcttctgt gctgtcagat atgtccagaa tcatggctca gatagcttta atgaaagcgg
    13321 taaaaggcat tgcttccgcg ctgccttttg attttgtagc taatgctgat ggcggtgttt
    13381 atcagtcggc tgatttgagt cgctacagtg gcacggtggt taaccgtccg acgttttttg
    13441 cttttgcaaa aggcgcgggt gtgatggggg aagctgggcc tgaagccatt ctgccactgc
    13501 gtcgtggtgc tgacggtaag ctgggggttg tggcggatat tggtggttca ggtatggcga
    13561 tgtttgcccc gcagtacaac atcgagatca ataacgatgg cacgaacggg cagataggtc
    13621 cggctgccct gaaggtggtt tatgaccttg ggaaaaaagc ggcagcggac tttatgcaac
    13681 agcaggcccg tgatggtggt cggttaagtg gagcatatcg gtaatggaga cgtttcactg
    13741 gaaagtgcgc ccggatatga atgtggtatc agagccgaaa gtggtgacag tgaagctggg
    13801 cgatggttat gaacagcgtc gtgcggcggg actgaataac cagttgtcga cttacagcgt
    13861 gacgatacgt gttcgtaaat gtgaacaccc atctttaaaa gcctttctgg aacggcacgg
    13921 tggcgtccgc gcatttcagt ggacgccacc ttatgactgg aagccgatca gggtggtttg
    13981 tcgtaaatgg tcggcaagcg tgggggcgct gtgggtaacc ataacggcag attttgaaca
    14041 ggtcgtggca taggaggccc tgatgcagga tattccacag gaaacacatc atgagacgac
    14101 acgccttact cagtcagccc aggtggtgct ctgggaaatc gatctgacag aggtcggtgg
    14161 tgaacgttat tttttctgta atgagcagaa cgaaaaaggt gagccggtca cctggcaggg
    14221 gcggcagtat caggtatacc ctattcaggg gacgggattt gaactgaacg gtaagggtag
    14281 tgctgcccgt ccgacactga cggtctctaa cctgcacggc atggtcaccg ggatggtgga
    14341 agacctgcag agtctggtcg gcggaacggt ggtcaggcgt aaggtttacg cccgttttct
    14401 ggatgcggtg aacttcgtca acggaaacag cgacgccgat ccggagcagg aggtgatcag
    14461 ccgctggcgc atcgagcagt gcagcgaact gagtgcggtc agtgcctcct ttgtgctctc
    14521 cacaccgacg gaaacggatg gtgctgtttt tccggggcgc atcatgctgg ccaacacctg
    14581 cacctggacc tatcgcggtg atgagtgcgg ttatcatggt ccggctgtcg cggatgaata
    14641 tgaccagccg acgtccgata tcacgaagga taaatgcagc aaatgcctga gcggctgtaa
    14701 gtttcgcaat aacgtcggca actttggcgg cttcctttcc attaacaaac tttcgcagta
    14761 atcccatgac acagacagaa tcagcgattc tggcgcacgc ccggcgatgt gcgccagcgg
    14821 agtcgtgcgg cttcgtggtg agaacgccgg aaggggaaag atattttccc tgcgtgaata
    14881 tctccggtga gccggaggcg tatttccgga tgtcgccgga ggactggctg cgggcagaga
    14941 tgcagggtga gattgtggcg ctggtccaca gccaccccgg tggcctgccc tggctgagtg
    15001 aggctgacag gcggctgcag gtgcagagcg atttgccttg gtggctggtc tgccggggtg
    15061 agattcataa attcagctgt gtgccgcatc ttaccgggcg gcgctttgag cacggggcga
    15121 cggactgtta cacgctgttc cgggatgctt atcatctggc ggggattgag atgccggact
    15181 ttcatcgtga ggatgactgg tggtgtaacg gccagaatct ctatctggat aatctggagg
    15241 ccacagggct gtatcaggtg ccgttgtcag cggcgcagcc gggcgatgtg ctgctgtgct
    15301 gttttggttc atcggtgccg aatcatgccg ccatttactg cggcgacggt gagctgttgc
    15361 accatattcc tgaacaactg agcaaacgag agaggtatac cgacaaatgg cagcgacgca
    15421 cacactccct ctggcgtcac cgggcatggc acgcatctgc ctttacgggg atttacaacg
    15481 atttggccgc cgcatcgacc ttcgtgtaaa aacgggggct gaagccatcc gggcgttgtc
    15541 cacacagctc ccggcgtttc gtcagaaact gaatgacggc tggtatcagg tgcgcattgc
    15601 cgggcgtgat gcaggtgaaa ccgaattatc tgcccgtctt aatgagccgc tggcaaatgg
    15661 tgccgtgatc cacatcgtgc cgcgtctggc gggagctaaa agtggcggtg tgtttcaggt
    15721 ggtgctgggg gcggcgctga ttgcggtggc atggtggaac cctgtgggct ggctgggtgc
    15781 cgcggctgta tcgggcatgt atgcggcagg ggccagtatg atcctgggtg gtgtggccca
    15841 gatgctggca ccgaaagccc ggacgcccac agcgaccagc acggataacg gtaagcagaa
    15901 cacctatttc tcatcactgg ataacatggt tgcccagggc aatgttctgc cggttctgta
    15961 cggtgaaatg cgcgtggggt cacgcgtggt ttctcaggag atcagcacgg cagacgaagg
    16021 ggatggtggt caggttgtgg tgattggtcg ctgatgcaaa atgttttatg tgaaaccgcc
    16081 tccgggcggt tttatcgttt atggagcatg acgaatgggt aaaggcagca gtaaggggca
    16141 taccccgcgc gaagcgaagg acaacctgaa gtccacgcag ctgctgagtg tgatcgatgc
    16201 catcagcgaa gggccggttg aaggtccggt ggatggatta aaaagcgtgc tgctgaacag
    16261 tacgccggtg ctggacagtg aggggaatac caatatctcc ggtgtcacgg tggtgttccg
    16321 ggcaggcgag caggagcagt caccgccgga gggatttgaa tcctccggtt ccgagacggt
    16381 gctgggtacg gaagtgaaat atgacacgcc gatcacccgc accattacgt cggcaaacat
    16441 cgaccgtctg cgctttacct tcggtgtaca ggcactggtg gaaaccacct caaagggtga
    16501 caggaatccg tcggaagtcc gtctgctggt tcagatacag cgtaacggtg gctgggtgac
    16561 ggaaaaagac atcaccatta agggcaaaac cacctcgcag tatctggcct cggtggtggt
    16621 ggg
//
LOCUS       DADTFG010000112        16275 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.113, whole genome shotgun sequence.
ACCESSION   DADTFG010000112 DADTFG010000000
VERSION     DADTFG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.113"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            92..196
                     /locus_tag="J1138_003338"
     CDS             92..196
                     /locus_tag="J1138_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBA8455100.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            506..2704
                     /gene="speF"
                     /locus_tag="J1138_003339"
     CDS             506..2704
                     /gene="speF"
                     /locus_tag="J1138_003339"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBA8455101.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     HGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            2701..4020
                     /gene="potE"
                     /locus_tag="J1138_003340"
     CDS             2701..4020
                     /gene="potE"
                     /locus_tag="J1138_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBA8455102.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4077..5717)
                     /gene="pgm"
                     /locus_tag="J1138_003341"
     CDS             complement(4077..5717)
                     /gene="pgm"
                     /locus_tag="J1138_003341"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBA8455103.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVAEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5743..6288)
                     /gene="seqA"
                     /locus_tag="J1138_003342"
     CDS             complement(5743..6288)
                     /gene="seqA"
                     /locus_tag="J1138_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBA8455104.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6473..7237
                     /gene="ybfF"
                     /locus_tag="J1138_003343"
     CDS             6473..7237
                     /gene="ybfF"
                     /locus_tag="J1138_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA8455105.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNNHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7308..7670
                     /gene="ybfE"
                     /locus_tag="J1138_003344"
     CDS             7308..7670
                     /gene="ybfE"
                     /locus_tag="J1138_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBA8455106.1"
                     /translation="MYYGALSIRAGAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7810..8340
                     /gene="fldA"
                     /locus_tag="J1138_003345"
     CDS             7810..8340
                     /gene="fldA"
                     /locus_tag="J1138_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBA8455107.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8550..8636
                     /gene="uof"
                     /locus_tag="J1138_003346"
     CDS             8550..8636
                     /gene="uof"
                     /locus_tag="J1138_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBA8455108.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8629..9075
                     /gene="fur"
                     /locus_tag="J1138_003347"
     CDS             8629..9075
                     /gene="fur"
                     /locus_tag="J1138_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBA8455109.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9159..9485)
                     /locus_tag="J1138_003348"
     CDS             complement(9159..9485)
                     /locus_tag="J1138_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455110.1"
                     /translation="MKKLILIAMIASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9535..10941)
                     /gene="chiP"
                     /locus_tag="J1138_003349"
     CDS             complement(9535..10941)
                     /gene="chiP"
                     /locus_tag="J1138_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBA8455111.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAIFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11383..13047)
                     /gene="glnS"
                     /locus_tag="J1138_003350"
     CDS             complement(11383..13047)
                     /gene="glnS"
                     /locus_tag="J1138_003350"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBA8455112.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13267..14028)
                     /locus_tag="J1138_003351"
     CDS             complement(13267..14028)
                     /locus_tag="J1138_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8455113.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(14030..15016)
                     /locus_tag="J1138_003352"
     CDS             complement(14030..15016)
                     /locus_tag="J1138_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HBA8455114.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAAAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(15009..16244)
                     /locus_tag="J1138_003353"
     CDS             complement(15009..16244)
                     /locus_tag="J1138_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBA8455115.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEMHYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWISVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
BASE COUNT         3938 a         4168 c         4034 g         4135 t
ORIGIN      
        1 atcgtttttt gttgatcttc ttcacagatt atagctattt catggataga ataaccctac
       61 cttcaactga cacagaaaaa ggtaaaggta aatggaaaat aataaccgct taatgcctca
      121 tataaggcgg acaacccata tcatgaagtt tgcccatcgt aatagttttg actttcattt
      181 ctttaatgcc cgctagtctt ctgactaaag ggcaccccag cgtacaggtc tccctgactt
      241 taagcattac aggttaatac ctgtatccct cggtgctcat atactgctaa ccctttcaaa
      301 ctctaaataa ttcgagtcgc agcacatgca acttgaagta tgacgagtat agccagttac
      361 cgggctggtc tgggttattg catctgcaaa aagcaaacta ctgatttatt tatcagcggt
      421 ggggttttgc ttttttccgg cgtgatcaat ttctcctttg agaaattgag gacctgctat
      481 tacctgaaat aaagagatga acaaaatgtc agaattaaaa attgccgtta gccgttcttg
      541 cccggattgt ttttccactc atcgtgaatg cgtgaatatt gacaaaagta attatattga
      601 cgttgccgcc attattttat cagtcaatga tgttgaacat ggaaaactcg atgaaataga
      661 cgctactggc tatggcattc ctgtttttat tgcaacggaa aatgaagaac gtgtaccagc
      721 agaatatctg ccacgtattt ctggtgtctt cgagcattgc gaatcacgga aagagtttta
      781 tggacgtcag ttagaaaccg ctgccagcca ttatgaaacg caactgcgcc cgcccttctt
      841 ccgcgcactg gtcgattatg tcaatcaggg caatagcgct tttgactgcc cgggtcatca
      901 gggaggtgag tttttccgtc gccatccggc aggcaatcag ttcgtggaat attttggcga
      961 aatgctcttc cgctccgact tgtgtaatgc cgacgttgca atgggcgatc tactgattca
     1021 tgaaggtgcg ccatgcattg cacagcaaca tgcggctaaa gtgtttaatg ccgataaaac
     1081 gtactttgtt ttaaacggca cctcctcttc caacaaagtg gttttaaacg ccctgctgac
     1141 accgggtgat ctggtgctgt ttgaccgcaa taaccataag tctaaccacc acggggcctt
     1201 gctacaggct ggtgcaacac cggtgtatct ggaaacagca cgtaacccgt atggttttat
     1261 cggtggcatt gatgcgcact gcttcgaaga aagttatctg cgtgaactga ttacagaagt
     1321 tgcaccgcaa cgggcaaaag aggcgcgtcc gttccgcctc gctgtgattc agttaggcac
     1381 ctacgacggt actatttata acgcccgcca ggtggtggat aaaatcggtc atctgtgtga
     1441 ctacatcctg tttgactcag catgggtcgg ctatgaacag tttattccga tgatggcgga
     1501 ctgttcgccg ctgctactgg agcttaatga gaacgatccg ggtattctgg ttacgcaatc
     1561 tgtgcataaa caacaggctg gtttttctca gacttcacaa attcataaaa aagacagcca
     1621 tatcaaaggg caacagcgtt atgtaccgca caaacgcatg aacaacgcct ttatgatgca
     1681 cgcctccacc agcccgttct atccgctgtt tgccgcactg gatatcaacg ccaaaatgca
     1741 tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg gttaacggta ttaatgcccg
     1801 caaattgatc ctcgataact gccagcacat tcgcccgttc gttcctgaac tggtggatgg
     1861 taaaccctgg cagtcgtatg aaacagcgca aattgcggtt gatctgcgct tctttaaatt
     1921 tgtacccggt gagcactggc attcttttga aggctacgca gagaaccaat actttgtcga
     1981 tccgtgcaaa ctgttgctga caactccagg tattgatgca cgtaacggcg aatatgaagc
     2041 gttcggtgta cccgcaacga ttcttgctaa cttcctgcgc gaaaatggcg tagtgccgga
     2101 aaaatgcgat cttaactcca tcctcttcct gctgactccg gcagaagata tggccaaact
     2161 gcagcaactt gttgccctgc tggtacgctt cgaaaaacta ctggaagctg atgcgcccct
     2221 ggcggaagta ctgccttcca tctacaaaca gcatgaagag cgctacgccg gttataccct
     2281 tcgtcagttg tgccaggaaa tgcatgattt gtatgcccgc cacaacgtga aacaactgca
     2341 aaaagagatg ttccgtaagg aacacttccc acgcgtcagc atgaatccgc aagaagccaa
     2401 ctacgcctac ttacgcggtg aagtggagct ggttcgtctg ccggatgcag aaggccgtat
     2461 cgctgccgaa ggtgcgcttc cttatcctcc gggtgtgctg tgtgttgttc ctggtgaaat
     2521 ctggggtggt gctgttctgc gttacttcag cgctctggaa gaaggcatca acctgctgcc
     2581 aggttttgca ccggagctgc agggtgtcta tatcgaagaa catgatggtc gtaagcaagt
     2641 ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcgcc ctgttgaaag gggaaaaatt
     2701 atgagtcagg cgaaatcgaa caaaatgggt gtcgttcagt taaccatact gacgatggtc
     2761 aacatgatgg gctccggtat catcatgctg ccgacaaagc ttgccgaagt cgggacaatc
     2821 tcaattatct cctggctggt gacagccgta ggctcaatgg cactggcatg ggcattcgca
     2881 aaatgcggta tgttcagccg taaatcagga ggtatgggcg gttatgccga gtatgctttc
     2941 ggtaaatccg gtaactttat ggcgaactat acctacggcg tctcgctgct gattgctaac
     3001 gtcgcgattg ctatttcggc ggttggttac ggcaccgaac tgctcggcgc gagtttgtcg
     3061 ccagtgcaga ttggtcttgc gaccatcggg gtgctgtgga tttgtaccgt ggctaacttt
     3121 ggtggtgcgc gcattaccgg gcaaatcagt agcattaccg tgtggggggt cattattccg
     3181 gtggtaggcc tgtgcattat cggctggttc tggtttagcc cgacgcttta cgttgattcc
     3241 tggaatccgc atcatgcacc gttcttcagt gcggtaggtt cttccatcgc tatgacgctg
     3301 tgggcttttc ttggtctgga gtctgcgtgt gcgaatactg atgtagtgga aaacccggaa
     3361 cgtaatgtgc caatcgcggt actcggcggt acgttaggtg cggcggtgat ttatatcgtc
     3421 tccaccaacg tgattgccgg gattgtgcca aatatggagc tggcaaattc aacggcacca
     3481 tttggtctgg ccttcgcgca gatgttcacg ccggaagtgg gtaaagtcat tatggcgctg
     3541 atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc ccaggtgttt
     3601 aaatcttctg ccgatgaagg gtacttcccg aaaattttct cccgtgtgag caaagtcgat
     3661 gcaccagtac agggaatgct gaccattgtc atcatccaga gcggattatc actgatgacc
     3721 attagcccgt cgctgaacag tcagttcaac gtgctggtta acctggccgt ggtgaccaat
     3781 atcatcccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt cgctaatgtg
     3841 cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat gtacagcttt
     3901 tatgcgctgt actcatccgg ggaagaagcc atgctatacg gttccatcgt gaccttcctc
     3961 ggttggacac tgtatggtct ggtctcacca cgctttgaac tgaaaaataa acacggttaa
     4021 ttaagcaaag caaaaaaggg cgatcttccg accgcccttt ttcttatttg atatgtttac
     4081 gcgtttttca gaacttcgct aacaatctca accgcttctt tctcaatctg cttgcgatgt
     4141 tcttcaccaa ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc
     4201 gcggcgaacc agccgttgtc agtcatcact ttcagaccgc cgatagaagc gccattaccc
     4261 ggtgccgccg tcagacgcgc ggtgatcggg tcacctgcca gggtgctggc gctcaccatt
     4321 tccggagaca gcttagacaa cgccgctttt tgtgcggaag tcgctgctgc ctgcaaacgg
     4381 ttatagctcg gcgcaccaaa gcgttttgcc agttcgttat agtgttcctg cgggttctta
     4441 ccggtgacag cggtgatttc cgccgccagc agacacataa tgatgccgtc tttatcggtg
     4501 gaccacggcg tgccgtcgaa acgcaggaag gaagcgcctg cactctcttc gccgccaaag
     4561 ccgaagctgc catcgaacaa accatcaaca aaccatttga aacctaccgg tacttctacc
     4621 agcttacggc ccaaatcatt gaccacacgg tcgatcatcg cagatgaaac cagcgtttta
     4681 ccgacagcaa catctttgcc ccactgcgga cgatgctgga acaggtagtt gatcgccacc
     4741 gccaggtagt ggttcggatt catcaaacct gccggagtga cgataccgtg acggtcataa
     4801 tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca gtgccagcag gcccgccatc
     4861 gcacactcgg aggagcagtc catacggatc gcaccgtctt tatcgaggtg cataaagcgg
     4921 aaggtttgat cgacctgatc gttaacgatg gtcaggttga ggttgtaata ctcgccaatg
     4981 cgcttccagt attcgatacc ggaaccaccc agcggatcaa cgcccagcgt caggcccgct
     5041 ttctgaatcg ccgccatatc aacgatatcg gccagccctt ccacaaacgg ctgcaccaga
     5101 tcctgctctt tcacatgacc ggatgccatc gcttcgtcga gggagatacg cttcacgcct
     5161 ttcaggccat cagccagcag tgcgttggct ctgtcttccg ccactttagt gacgttggta
     5221 tcagccgggc caccatttgg cggattgtac ttgataccac catcttccgg cgggttatgg
     5281 gacggtgtaa tcacgatacc gtctgccagc gggccacctt ttttattgtg aaccagaatg
     5341 gcattggaaa tggcaggtgt cggagtgaag ccattgtttt cctgcacaat gacatcaacg
     5401 ccgttcgctg ccagcacttc cagtacggaa atgaacgcag gctcggacag ggcgtgagta
     5461 tctttaccca catagcaagg gccagtgatg ccgtttttcg cacgttcttc agcaattgcc
     5521 tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc gcgctgcact gccacggtga
     5581 ccggaagtac cgaatttcac cgcgtgctcc gcattccctg cttctggttt cagtacataa
     5641 tattgcgccg tcagttgggc gacgttaatc aaatcactct gttgtgcagg ttggcctgca
     5701 cgattgtgga ttgccattgc tttgtccttt gtctgcaacg ttttagatag ttccgcaaac
     5761 cttctcaatc aattccgccg ggaattgcat cgactgcatg atgtgttcga tcatgctgca
     5821 tttacggccg gtgttggtgt tggtgatcac ccaatacggc gtaccaggca catgtttcgg
     5881 cttggtctga ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt
     5941 acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt
     6001 agacaacagc agcataaagc gattgaccgc tcgcttttgc tctgcgtatt cgtccgaaag
     6061 cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac
     6121 gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc
     6181 ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg
     6241 gctggcaata tagctgtaga gttcatcatc aacttcaatc gttttcatct taatccagtg
     6301 cagtgtctta gctgaataca agtcgccaag agtatatgaa taaatcccgg cagcggatag
     6361 cgtcaagcct ggcttgcaga aaaactcgga ataaacctta tttgttgcag ccctgtggca
     6421 caatggtcaa cataataccc taacccgaca acgcgaaaaa aagaactttg ccatgaaatt
     6481 gaatatccgc gcgcaaactg cacaaaacca gcacaataat tctcccatcg ttcttgtcca
     6541 tggtctgttt ggcagcctcg acaaccttgg cgtactggct cgcgatctgg taaacaatca
     6601 caatatcatc caggttgata tgcgtaacca cggtctttca ccgagagatc cggtaatgaa
     6661 ttacccggcg atggcgcagg atcttgttga taccctggat gcacagcaga tcgacaaagc
     6721 aacatttatc ggtcactcca tgggtggtaa agcggtaatg gctcttactg cactagcccc
     6781 cgatcgcatt gacaagctgg tggcgattga tattgcgccg gtcgattatc acgttcgccg
     6841 tcatgatgag attttcgcgg ccatcaacgc agtcagtgaa tcagacgcac aaacacgcca
     6901 gcaagcagcg gcaataatgc gccagcatct taatgaagaa ggggtgattc agtttctgtt
     6961 gaaatctttt gttgacgggg agtggcgctt taacgtgcca gtattgtggg atcagtatcc
     7021 gcatattgtg ggttgggaga aaatcccggc atgggatcat cctaccctgt ttatccctgg
     7081 cggcaattct ccgtatgtta gcgagcagta ccgtgatgat ttactggcac aatttccaca
     7141 ggctcgggcg catgtgattg caggcgcggg tcactgggtc catgctgaaa aaccggatgc
     7201 ggtattacgc gctatccgtc gctatctcaa tgattaacgc aactgattaa aaacccagcg
     7261 tccgcgtcgc tttccgcgcg tggacgttgg cgtgactcat cgcgctgatg tattatggcg
     7321 cgctatccat ccgggcaggc gcctggctga ttgtttcccc cgaagtcacc aagatcatgg
     7381 ccaaagaaca aacggaccgt acgacattag atctgttcgc gcacgagcgt cgaccgggac
     7441 gaccgaaaac taatccgctt tcgcgcgatg agcagctgcg tattaataaa cgcaaccaac
     7501 taaaacgcga caaagtacgt ggccttaagc gtgtcgaact gaagctgaac gcggaagctg
     7561 tcgaggcact gaacgagctg gcggaatcgc gcaatatgag ccgtagcgag ctgatcgaag
     7621 agatgttaat gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa atttccactt
     7681 tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg cctgttctgc
     7741 tatgattgcc tttatccgtg ggcaattttc cacccccatt tcaataagtt tcaagaggtt
     7801 atttcactca tggctatcac tggcatcttt ttcggcagcg acaccggtaa taccgaaaat
     7861 atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg ccgatgtcca tgacattgca
     7921 aaaagcagca aagaagatct ggaagcatat gacattctgc tgctgggcat cccaacctgg
     7981 tattacggcg aagcgcagtg tgactgggat gacttcttcc cgactctcga agagattgat
     8041 ttcaatggta aactggttgc gctgtttggt tgtggcgacc aggaagatta cgcagaatac
     8101 ttctgcgatg cattaggcac catccgcgac atcattgaac cgcgcggcgc aactatcgtt
     8161 ggtcactggc caaccgcggg ctaccacttc gaagcgtcaa aaggtctggc tgatgatgac
     8221 cactttgtcg gtctggctat cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta
     8281 gaaaaatggg ttaaacaaat ttctgaagag ctgcatctcg acgaaattct caatgcctga
     8341 tgtgatgcgg cgtagactca tgtctacgcc gtattaatag ataatgccaa tcaaaataat
     8401 tgctacaaat ttgtaacttt tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc
     8461 ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac aatatttgcc agggacttgt
     8521 ggttttcatt taggcgtggc aattctataa tgatacgcat tatctcaaga gcaaattctg
     8581 tcacttcttc taatgaagtg aaccgcttag taacaggaca gattccgcat gactgataac
     8641 aataccgccc taaagaaagc tggcctgaaa gtaacgcttc ctcgcttgaa aatcctggaa
     8701 gttcttcagg agccggacaa ccatcacgtc agtgcggaag atttatacaa acgtctgatc
     8761 gatatgggtg aagaaattgg tctggctacg gtatatcgcg tactgaacca gtttgacgac
     8821 gctggtatcg tcacccgcca caattttgaa ggcggtaaat ccgtatttga actgacacag
     8881 caacatcacc acgatcacct gatctgcctc gactgcggta aggttatcga atttagtgat
     8941 gattccatcg aagcgcgtca gcgtgaaatc gccgcaaaac atggcattcg cctgactaac
     9001 cacagtctct atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga tgagcacgcg
     9061 cacgaaggca aataagccag cctgaacgag aaaagccaac ctgcaggttg gcttttttat
     9121 acaaggaaaa taagaaaagc gagagttaca gctctcactt atttgttatt actgcgaatt
     9181 tcctgccaga ccttatcaca atcggctttc accgcctgat catttccggt ttgcgttgcg
     9241 cgcagacact gctgataatc cagtacgcgt acactttcct gctcggcaaa ctgctgatgt
     9301 tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttccggcgaa
     9361 ccttgcgcgg tgttgataca ggcgctgtaa gcctctttca gacgactgtc ttcctgcggt
     9421 gcggttgatt gcgcacaagc taccagcccc gatgcaatca tggcgatgag aatcagtttt
     9481 ttcatagcaa attcctcaaa tgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa
     9541 gatggtgaat ggtgcgatta ccataaattt cacgtcacgc tcatcctgga agatgttgcc
     9601 gtaaccacca ccccagcttg ggatgtcgga gtggttgtcg tattcggtga agtgcagttt
     9661 gaacatcgtg cctttggcgc gaccgtcctg aatggtgtac accgcatcca ggctgtaggc
     9721 agactcttca atagtccggt ttttatcgta gtacgcatcc ggattgctct gccaggtcgc
     9781 aggtttagca tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg gaagattcca
     9841 gtttttcagg tcatacatcg caccgaagaa gatcgctttt tcgccgttgg cgttgaagtc
     9901 ggaacggtta tcccaccaga tatccaggcg accgttcgag gaagcgtagg ttggagtcat
     9961 acgttgcagg aagtatcctt gctgaccgtc agccttaacc caggtacctt cgaggcgcaa
    10021 atccactacg tcagccgccc ggtaaccaaa ggtcaacgcc tgcagccagg cggtgccgtc
    10081 ataaagatcg ttgacgctgc gatcgtcaac tttatcgcgc gtaccataga actggtagct
    10141 ggtggttaac gggctaccgg cgatatcaaa tttgtagctg gctttggcaa aatattgatc
    10201 gatataccct tcggcctgac caaacgccgc ttccagtacg aagttatttt tgaagtcgta
    10261 cttcgcccca agggagtgta gataatcaac tttggtggtt ttatcgttct gataaaactc
    10321 atccatttcc agatgccacg gtgctttgta ttcgttggtc cacatgtagg agaaactcaa
    10381 cgcaccagca tcgccgtaat caaaattcgc cccggcctcc gccccctgat aagtacccgg
    10441 cataaagctc cagtgtggcg ctaacagcgt ctgaccggtt ggctgaatat aacctgccct
    10501 cgcccaaacc ggaccgtatt taaatttggc cgctgcttta tacaggctta taccgctttt
    10561 atcgccggac cagtcttcgt cataggcttt attacttttt gaaaacgcga tttcgttcgg
    10621 gtgagagctg tcgccgtttt ccgccatttc aatcgccgta aacacggcaa tatcaaggcc
    10681 gaacatatca gcagcataac cagactgaaa atcgaggttg gcgttccagg tggagtgaga
    10741 aaggttggtt ttgtatttat cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca
    10801 gtaatagata ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg
    10861 aattgccata aagcccgaca tagctgtaac accggcgata gccagcgcca gcgtactacg
    10921 tttgccacta aacgcacgca tgggttaatc ctctttgacg tataaattgc tgcaccaaag
    10981 gtgaacagcg aaaaaaataa aaattaaaaa agtagcgttg agaaaagtct cgctgactac
    11041 aggaacagac tatttttcgc gacgcgaatt atcaatctct ttctgtaacc agaatgtaag
    11101 aacatgagca accgcacata ttttgatgat ttttgttaca aggcttaagt ttgcagttga
    11161 aaggcttgca gaagaataaa agcgtgaatt tccagcaaga tgaaaacggt tacattttat
    11221 ttaattatgg tttgtgattt taaatttgtt ttatggataa attgcgaagc ggaattaatt
    11281 cgcgaagaaa agcagaaaaa aacgccgcag agcgcggcgt ttgggataat ttgcatattt
    11341 cttgccggat gcggcgtaaa cgctctatcc gacaacgtaa acttactcgc ctactttcgc
    11401 ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg tagaatggcg
    11461 gctgtcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg caaccgcatc
    11521 tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt taatcaccga
    11581 caggaaatca tccgcagcac cagggttcgg cacgctgaac agacggtcat acaaacggat
    11641 ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga ctttacgacc
    11701 atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg tggtgatatt
    11761 gccttcggca tctttctcga cgcgttctgc cttaatgacg taagcattac gcagacgcac
    11821 ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga aatcggcgcg
    11881 atcgatccaa atctcaccgc taaatggcac ctggcggctg cccatttccg gtttattcgg
    11941 atggttcggc atggtaacca tttcgccttc gccctggtag ttttcgataa ccagtttcac
    12001 cggatcgata accgccattg cgcgcggcgc attttcgttg agatcttcac ggatgcagga
    12061 ttccagtgac gccatctcaa tggtgttgtc ctgcttggtc acaccaatgc gtttgcagaa
    12121 ctcacgaata gaagccgcag tgtaaccacg acgacgcaga ccagaaatgg tcggcatacg
    12181 cgggtcatcc cagccttcaa cgtgcttatc ggtcaccagc aggttcagct tacgcttgga
    12241 catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat
    12301 ggtgatgttg tccagtaccc agtcgtacag acggcggttg tcctggaact caagcgtaca
    12361 cagagagtgc gtaatacctt ccagcgcatc gctgatgcag tgggtgaagt cgtacatcgg
    12421 gtagatgcac cacttgttgc cagtctggtg gtgttcagca aacttaatgc ggtacagcac
    12481 cggatcgcgc atcacgataa acggcgaagc catgtcgatt ttcgcacgca ggcaggcttt
    12541 accttcttca aaaccgccgg tacgcatttt ctcgaacagc gccaggttct cttcaacgct
    12601 gcgatcgcgg tacgggctgt ttttacccgg ctgcgtcaag gtgccgcggt attcgcggat
    12661 ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt cgatcgcata
    12721 ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc agtgaaaacc
    12781 taaccactct acgtcgtttt tgatcgaatc gacatactcg atatcttctt ttaccgggtt
    12841 agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc cgaagttcag
    12901 gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg ggaaacgggt
    12961 gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac gaataaagtt
    13021 agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac aacgtataac
    13081 ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagcgaaa aaatggtttt
    13141 gcgtaagata ttggggcgtt ttctgcgcta ctgccagcag atcctcgcgc tggaaacgta
    13201 aggccaaacg tcgccagcct gacgcattta ttaaattgcc ggatgagctg gccgcagagt
    13261 catagattag tcggctatga ttaaacgcgt cgacaaggac gcaagcgttc tacgcgtctg
    13321 cccatcaagc tgactatccg taataatctc tgtcagttca ttaagatgcg cgatattaaa
    13381 cagcgaccat gaaccgtatt ttgagctgtc agccaggaga attttgcgcg aagcatgcgc
    13441 gataagatcg cgttttagtg cggctttctc ttccgtgggt gctgtaatac ccttttcaag
    13501 atcccagctg ttacagctta aaaatgccac gtccggccag gtattttgca gcaatttacg
    13561 accatgttcg ccaatgcagg actggctgga gtcgtcgata cgtccgccga taatcgtcac
    13621 ttcaatttgc ttgaattcag ccaaaaaaag cgcgatatgc aggtcgctgg tgatcacgcg
    13681 aagcggtaaa tgtgtaatct gccgcgcaag ttcaatcatt gtcgtaccgg catcaaggac
    13741 gacggaatcc cccgctttga ccattgacgc cgctgcgctg gcgatggcat gtttttccgc
    13801 caggctgcgc tgcattttct catgggtagt cggttgggta ggaataaacc ggttgagggt
    13861 cacaccgccg tgtgtacggc tgataacacc ctgctcatcg agttttatca ggtctcgacg
    13921 aatcgtagcc ggcgacgcat tggttaccgc caccagctca tcaacggtga ccagattatg
    13981 gcttttcagg taatccatga tctgctctaa ccggttatat cccttcattt tattctcgcg
    14041 aaatctgcat tgctaactgg atggaaactg ccatgctctc agacttggct tttcctgtcc
    14101 aggcaatatc aaacgcggtg ccgtggtcgg cagaagtgcg aataaatggc aacccggcgg
    14161 tgatattcac cccatcataa aatccaagca gttttaaagg gatatgcccc tgatcgtggt
    14221 acatagccac taccatatcg aatagccctt catggcattg cataaacacg gtatctggcg
    14281 ggcacgggcc agtaacatca atatcttcgg cctgcattgc ttcaatcgca ggcgcgataa
    14341 tctcgatttc ctcagtgccg aataaaccat gttcccctgc atgtggattt acacccgcca
    14401 cggcaatacg cggacgctca ataccaacac gtttcaggaa gtggttcgca actcgaataa
    14461 ccgttttgac cctttcacca ttcaaagtat ccaggaattt acgcagtgag atgtgagtcg
    14521 aaatatggat aaccttgagc ttatcggtat agagcaccat cgcgtactct ttgctgccgg
    14581 taagatgggc cagcaattcc gtatgccccg gatagttatg cccgccaaga tgcagcgctt
    14641 ctttattcag cggcgcagtg gctatggctt tcacctcgcc tgataaggcc agagccgttg
    14701 cacgtttgat acagcgatac gctaaatcgc ctgccgcagc ctgtaccact ccgggtgtca
    14761 gcgcatcggg gttagccagc ggctcatcca tcacgttaat cacgccggga gcaaaatggg
    14821 cgtcgcacac cttttgaatg acgcgtaact ctgcctgcgg cgtgatattc atcgccataa
    14881 tacgtcgcag cgtacgcaca cagcccacca ccaccaccgg ggcaccagca agcccggctt
    14941 cgctcaggga tttaataatg atctccgggc caatacccgc cgggtcaccc atcgttaccg
    15001 caataatatt attcactaca cttctcctcg ataaaattca gaacctgatg caacgtggtc
    15061 tcatcgccaa acccaccggc tttcgtcatc accgaacggg accagacacc gcccagaaag
    15121 tgaccgtatg gaacgcattg cgccaccttc cccgtaatac gaaatccggt cgcgcctaac
    15181 gcactggctg ttgccattgc gacatcacca ccagagagat aaagcgcatc gggcattgtg
    15241 cgaagcagaa cttgccgggt aagttcgcca agaaaccggc aaattttttc cccaagtgca
    15301 gcgcgactca tctgccaacg ctggcaaagg gtgtcgatct gatgccttgc tacagaatcg
    15361 ttacaggtgt ggacaatgca gtggtcgcct ttctgcaagg cctgcacaat acgcgcatca
    15421 tagtcactgg cattgcctgc cagaatgtgt tcaacatcga cgtatatctg tgtgatacgt
    15481 gggtgtgaat gaagtgtggc gatctgccgt tgagcgattt cgctcattga accaataatg
    15541 gccagcacgg tgggtggcgg cataatcgcg gagcgccggg caacggcatc acaaatacca
    15601 gcggagccga ccagcagcag cggttgttta gcctgcatca cagcggcagc gatgcgatcc
    15661 aggtcgctgt ccgtctgcgc atctatcacg ctaatccacg gttgctcata agacaaatgg
    15721 ctgagctgag aaaccgtaac gggctggcat ggaatggccg tctgcaaccg gatgatgtcc
    15781 gcaattcggg cgctcaatac cggggttttc ggatcgctgg caaactcggt ttccgttatc
    15841 cgcacaccgt ttacccaaca ctcaccggcc actgtggtcc ttcccgcctg aggaaatgcg
    15901 ggggcaacca ctgcgcccgt tataccgcat gccttcatca gggcttcaat ttctgcgccg
    15961 atattgcctc gcagcgtgga atctatcttt ttaatcacga gcggcggatt attcgccaga
    16021 gggagatgac tcatcagcga ggttattttc atcgccgcca gcttaggatc catcgcccgg
    16081 ctgtcgctgt taatcaccca tacgctggca tcaccgcgat aatgcatttc aaaggccaca
    16141 tcaactgtat gccccacctg cgccaggctt acccccgcgt cgttggctcc cgtgaaatca
    16201 tcggcaacga cgagaatgct cggcttagtg attccctttt ccatcatcac tccaggttgt
    16261 tttatgataa caatg
//
LOCUS       DADTFG010000113        15900 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.114, whole genome shotgun sequence.
ACCESSION   DADTFG010000113 DADTFG010000000
VERSION     DADTFG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.114"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..1770
                     /gene="ftsK"
                     /locus_tag="J1138_003354"
     CDS             <1..1770
                     /gene="ftsK"
                     /locus_tag="J1138_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBA8455116.1"
                     /translation="QPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQP
                     VAPQPQYQQPQQPVTQQPQYQQPQQPVVPQPQDTLLHPLLMRNGDSRPLHKPTTPLPS
                     LDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPG
                     VKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFR
                     DNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPED
                     VRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRN
                     LAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGK
                     KVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTIL
                     DQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITS
                     DSESEGGVGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQME
                     AQGIVSEQGHNGNREVLAPPPFD"
     gene            1929..2540
                     /gene="lolA"
                     /locus_tag="J1138_003355"
     CDS             1929..2540
                     /gene="lolA"
                     /locus_tag="J1138_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBA8455117.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            2551..3894
                     /locus_tag="J1138_003356"
     CDS             2551..3894
                     /locus_tag="J1138_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBA8455118.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            3985..5277
                     /gene="serS"
                     /locus_tag="J1138_003357"
     CDS             3985..5277
                     /gene="serS"
                     /locus_tag="J1138_003357"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBA8455119.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            5516..7960
                     /gene="dmsA"
                     /locus_tag="J1138_003358"
     CDS             5516..7960
                     /gene="dmsA"
                     /locus_tag="J1138_003358"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBA8455120.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATKMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGISNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            7971..8588
                     /gene="dmsB"
                     /locus_tag="J1138_003359"
     CDS             7971..8588
                     /gene="dmsB"
                     /locus_tag="J1138_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBA8455121.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            8590..9453
                     /locus_tag="J1138_003360"
     CDS             8590..9453
                     /locus_tag="J1138_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBA8455122.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELANIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9489..10115)
                     /locus_tag="J1138_003361"
     CDS             complement(9489..10115)
                     /locus_tag="J1138_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA8455123.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            10429..11577
                     /locus_tag="J1138_003362"
     CDS             10429..11577
                     /locus_tag="J1138_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8455124.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTHVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(11674..12414)
                     /gene="pflA"
                     /locus_tag="J1138_003363"
     CDS             complement(11674..12414)
                     /gene="pflA"
                     /locus_tag="J1138_003363"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBA8455125.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(12606..14888)
                     /gene="pflB"
                     /locus_tag="J1138_003364"
     CDS             complement(12606..14888)
                     /gene="pflB"
                     /locus_tag="J1138_003364"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBA8455126.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(14943..15800)
                     /gene="focA"
                     /locus_tag="J1138_003365"
     CDS             complement(14943..15800)
                     /gene="focA"
                     /locus_tag="J1138_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBA8455127.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         3855 a         3868 c         4231 g         3946 t
ORIGIN      
        1 cagccgcaac aaccggtagc accgcagccg cagtatcagc agccgcaaca accggttgca
       61 ccgcagccgc agtatcagca gccgcaacaa ccagttgcgc cacagccgca atatcaacag
      121 ccgcaacaac cggttgcacc gcagccgcaa tatcagcagc cacaacagcc ggttacacag
      181 cagccgcagt atcagcagcc gcaacagccg gttgtaccac agccgcagga taccctgctt
      241 catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc ataaaccgac gacgccactg
      301 ccttctctgg atttgctgac accgccgccg agcgaagtgg agccggtaga tacctttgcg
      361 cttgaacaaa tggcgcgcct ggtagaagcg cgtctggctg atttccgtat taaagccgat
      421 gttgtcaatt actctccggg gccagttatc actcgctttg aattgaacct ggcaccgggc
      481 gtaaaagcag cgcgcatttc taacttgtca cgtgaccttg cccgttcact ttcgacggtg
      541 gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg taggtctgga gttaccgaat
      601 aaaaaacgac aaaccgttta tctgcgcgaa gttttggata acgccaaatt ccgcgataat
      661 ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg gtgagccggt ggttgccgat
      721 ctggcgaaaa tgccgcactt gttggttgcg gggactaccg gttccggtaa gtctgtcggg
      781 gtgaatgcga tgatcctgag catgctttat aaagcacagc cagaagatgt acgtttcatc
      841 atgatcgacc cgaaaatgct ggagctttcg gtttatgaag gcattccgca cctgttaacg
      901 gaagttgtta ctgatatgaa agatgccgcc aacgcgctgc gctggtgtgt taacgagatg
      961 gagcgtcgtt ataaactgat gtctgcgctg ggtgtgcgta atctggcggg ttataacgaa
     1021 aaaattgctg aagccgatcg catgatgcgt ccgattccag acccgtactg gaagccgggt
     1081 gatagtatgg atgcgcagca tccggtgctg aaaaaagaac catacattgt ggtgttggtg
     1141 gacgaatttg ccgacctgat gatgacggta ggtaaaaaag tggaagagct gatagcacgt
     1201 ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac tggcaacgca gcgtccatcg
     1261 gttgatgtta ttactggtct gattaaagcg aatattccga cccgtatcgc ctttaccgta
     1321 tccagtaaga ttgactcacg taccattctt gatcaggctg gtgcggaatc actgctgggt
     1381 atgggggata tgctctactc tgggccgaac tccacgttgc cggtacgtgt ccatggtgct
     1441 tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact ggaaagcgcg tggtcgccca
     1501 cagtatgttg atggcatcac ctctgacagt gaaagcgaag gtggcgtggg cggtttcgat
     1561 ggcgctgaag aactggatcc gttgttcgat caggcggtgc agtttgtcac tgaaaaacgc
     1621 aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg gttataaccg tgcagcgcgt
     1681 attatcgaac agatggaagc gcaggggatt gtcagcgaac aggggcacaa cggtaatcgt
     1741 gaagtgctgg ccccaccgcc atttgactaa ttaatgcaac gtagaccgga taagacgcga
     1801 tagcgtcgca tccggtattc tatcaactga aaattcagta ttttcttctt tcctcaagct
     1861 gattattagc ctggaataga gagtagaggg aactcccgat tgggagtgac gtaatttgag
     1921 gaataatgat gaaaaaaatt gccatcacct gtgcattact ctcaagctta gtagcaagca
     1981 gcgtttgggc tgatgccgca agcgatctta aaagccgcct ggataaagtc agcagcttcc
     2041 acgccagctt cacacagaaa gtgactgacg gtagcggcgc ggcggtgcag gaaggtcagg
     2101 gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca tatgactcaa cctgatgaaa
     2161 gcattctggt ttctgacggt aaaacactgt ggttctataa cccgttcgtt gagcaagcta
     2221 cggcaacctg gctgaaagat gccaccggta atacgccgtt tatgctgatt gcccgcaacc
     2281 agtccagcga ctggcagcag tacaatatca aacagaatgg cgatgacttt gttctgacgc
     2341 cgaaagccag caatggcaat ctgaagcagt tcaccattaa cgtgggacgt gatggcacaa
     2401 tccatcagtt tagcgcggtg gagcaggacg atcagcgcag cagttatcag ctgaaatccc
     2461 agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac cccgccgcaa ggcgtcacgg
     2521 tagatgatca acgtaagtag aggcacctga gtgagcaatc tgtcgctcga tttttcggat
     2581 aatacttttc aacctctggc cgcgcgtatg cggccagaaa atttagcaca gtatatcggc
     2641 cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg ctatcgaagc cgggcatctg
     2701 cattctatga tcctctgggg gccgccgggt accggcaaaa caaccctcgc tgaagtgatt
     2761 gcccgctatg cgaacgctga tgtggaacgt atttctgccg tcacctctgg tgtgaaagag
     2821 attcgcgagg cgatcgagcg cgctcggcaa aaccgcaatg caggtcgccg cactattctt
     2881 tttgttgacg aagttcaccg tttcaacaaa agccagcagg atgcatttct gccacatatt
     2941 gaagacggca ccatcacttt tattggcgca accactgaaa acccgtcgtt tgagcttaat
     3001 tcggcactgc tttcccgtgc ccgtgtctat ctgttgaaat ccctgagtac agaggatatt
     3061 gagcaagtac taactcaggc gatggaagac aaaacccgtg gctatggtgg tcaggatatt
     3121 gttctgccag atgaaacacg acgcgccatt gctgaactgg tgaatggcga cgcgcgccgg
     3181 gcgttaaata cgctggaaat gatggcggat atggccgaag tcgatgatag cggtaagcgg
     3241 gtcctgaagc ctgaattact gaccgaaatc gccggtgaac gtagcgcccg ctttgataac
     3301 aaaggcgatc gcttttacga tctgatttcc gcactgcata agtcggtacg tggtagcgca
     3361 cccgatgcgg cgctctactg gtatgcgcga attattaccg ccggtggcga tccgttatat
     3421 gtcgcgcgtc gttgcctggc aattgcttcc gaagatgtcg gcaatgccga cccacgggcg
     3481 atgcaggtgg caattgcggc ctgggattgc tttactcgcg ttggcccggc ggaaggtgaa
     3541 cgcgccattg ctcaggcgat tgtttacctg gcctgtgcgc caaaaagcaa cgctgtctac
     3601 actgcgttta aagccgcgct ggccgatgct cgcgaacgtc cggattatga cgtgccggtt
     3661 catttgcgta atgcgccgac gaaattaatg aaggaaatgg gctacgggca ggaatatcgt
     3721 tacgctcatg atgaagcaaa cgcttatgct gccggtgagg tttacttccc gccggaaata
     3781 gcacaaacac gctattattt cccgacaaac aggggccttg aaggcaagat tggcgaaaag
     3841 ctcgcctggc tggctgaaca ggatcaaaat agccccataa aacgctaccg ttaatgttat
     3901 cgttgcggta atgttgttaa tgtatccctg tggtcgcagg ctgtggccac atctcccatt
     3961 taattcgata agcacaggat aagcatgctc gatcccaatc tgctgcgtaa tgagccagac
     4021 gcagtcgctg aaaaactggc acgccggggc tttaagctgg atgtagataa gctgggcgct
     4081 cttgaggagc gtcgtaaagt attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac
     4141 tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt
     4201 ctggaagtga acaaactggg cgaagagctg gatgcagcaa aagccgagct ggatgcttta
     4261 caggctgaaa ttcgcgatat cgcgctaacc atccctaacc tgcctgcaga tgaagtgccg
     4321 gtaggtaaag acgaaaatga caacgttgaa gtcagtcgct ggggcacccc gcgtgagttt
     4381 gactttgaag ttcgtgacca tgtgacgctg ggtgaaatgc actctggcct cgactttgca
     4441 gccgcagtta agttgactgg ttcccgcttt gtggtaatga aagggcagat tgctcgcatg
     4501 caccgcgcac tgtcgcagtt tatgctggat ctgcataccg aacagcatgg ctacagtgag
     4561 aactatgttc cgtacctggt taaccaggac acgctgtacg gtacgggtca gctgccgaaa
     4621 tttgctggcg atctgttcca tactcgtccg ctggaagaag aagcagacac cagtaactat
     4681 gcgctgatcc caacggcaga agttccgctg accaacctgg tacgcggtga aatcatcgat
     4741 gaagatgatc tgccaattaa gatgaccgcc cacaccccat gtttccgttc tgaagctggt
     4801 tcatatggtc gtgacactcg tggtctgatc cgtatgcacc agttcgacaa agttgaaatg
     4861 gtgcagatcg tgcgcccgga agactcaatg gcggcgctgg aagagatgac cggtcatgcg
     4921 gaaaaagtcc tgcagctgct gggcctgccg taccgtaaaa tcatcctttg taccggcgac
     4981 atgggctttg gcgcttgcaa aacttacgac ctggaagtat ggatcccggc acagaacacc
     5041 taccgcgaga tctcttcctg ctccaacgtt tgggatttcc aggcacgtcg tatgcaggca
     5101 cgttgccgta gcaagtcgga caagaaaacc cgtctggttc ataccctgaa cggttctggt
     5161 cttgctgttg gtcgtacgct ggttgcagta atggaaaact atcagcaggc tgatggtcgt
     5221 attgaagtac cagaagttct gcgtccgtat atgaacggac tggaatatat tggctaatac
     5281 ccaatttttc tgaatctaaa aagcgcctgc gggcgctttt tttgtctccc tttgataccg
     5341 aacaataatt actcctcact tacaagtaat actactttcg agtgaaaatc tacctatctc
     5401 tttgattttc aaattattcg atgtatacaa gcctatatag cgatctgcta tagaaataat
     5461 tacacaatac ggtttgttac tggaatcaat cgtgagcaag cttgagtgag ccattatgaa
     5521 aacgaaaatc cctgatgcgg tattggctgc tgaggtgagt cgccgtggtt tggtaaaaac
     5581 gacagcgatc ggcggcctgg caatggccag cagcgcatta acattacctt ttagtcggat
     5641 tgcgcacgct gtcgatagcg ctattccaac aaaatcagac gaaaaggtta tctggagcgc
     5701 ctgtacagtt aactgtggta gtcgctgccc gctacgtatg cacgtagtgg acggtgaaat
     5761 caaatatgtc gaaacggaca ataccggcga tgacaattac gacggcctgc accaggttcg
     5821 cgcctgtctg cgtgggcgtt ccatgcgtcg ccgtgtctac aatccggacc gcctgaaata
     5881 tccgatgaaa cgagtcgggg cgcgcggtga aggcaaattc gagcgcatta gctgggaaga
     5941 agcctacgac atcatcgcga ccaaaatgca gcgcctgatc aaagagtacg gcaacgagtc
     6001 tatctatctg aactatggca ccggtacgct gggcggcacc atgacccgct cgtggccgcc
     6061 gggaaatacc ctggtcgcgc ggctaatgaa ctgctgcggc ggttatctga accattatgg
     6121 cgactactcc tccgcgcaaa ttgcggaagg tctgaactat acctacggcg gctgggcaga
     6181 tggtaacagc ccgtcggata tcgaaaacag taaactggta gtgctgtttg gtaataaccc
     6241 tggcgaaacg cgaatgagtg gcggtggggt gacttactat cttgaacagg cgcgccagaa
     6301 atctaatgct cgcatgatca tcatcgatcc gcgctatacc gacaccggtg ccgggcgtga
     6361 agatgagtgg atccctattc gtccgggaac agatgccgca ctggttaacg gtctggcgta
     6421 tgtcatgatc accgaaaacc tggtagatca ggaattcctc gataaatatt gcgttggcta
     6481 cgacgagaaa accctgccag ccagtgcgcc gaaaaatggt cactataaag cttatattct
     6541 gggtgaagga cccgatggcg tggctaaaac accggaatgg gcctcgcaaa tcaccggtgt
     6601 tccggcagac aaaatcatca aactggctcg tgaaatcggc agtaccaaac cggcgtttat
     6661 cagccaggga tggggcccgc agcgtcatgc taacggtgaa atcgcaaccc gtgctatctc
     6721 gatgctggcg attctgaccg gtaacgttgg tattaacggg gggaacagcg gcgcgcgcga
     6781 aggttcatac agcttaccgt ttgtccgtat gccgactctc gaaaacccga tccagaccag
     6841 catttctatg tttatgtgga ccgatgccat tgaacgtggc ccggaaatga cggcgctgcg
     6901 tgatggtgtg cgcgggaaag ataagctgga tgtgccgatc aaaatgatct ggaactatgc
     6961 cggtaactgc ctgattaatc agcattctga aatcaaccgt acccatgaaa tccttcagga
     7021 tgataagaag tgcgagctga ttgtggttat cgactgccac atgacctcat cggcgaaata
     7081 tgctgacatc ctgctgcctg actgcaccgc ttccgaacag atggactttg cactggatgc
     7141 atcctgcggg aatatgtctt acgttatttt caatgatcag gtcattaaac cgcgctttga
     7201 atgtaagact atctatgaga tgaccagcga actggcaaaa cgtcttggcg tcgaacaaca
     7261 gtttactgaa ggtcgtactc aggaagagtg gatgcggcat ctgtatgccc agtcgcggga
     7321 agcgattcct gaactgccaa cgtttgaaga gttccgtaag caggggatct ttaaaaagcg
     7381 cgatccgcaa gggcatcacg ttgcttacaa agccttccgt gaagatccgc aggctaatcc
     7441 attgaccacg ccatcgggca aaattgagat ctattcacag gcgctggctg acattgccgc
     7501 tacctgggaa ttgccagaag gtgatgtgat cgatccactg ccgatctata cgccgggctt
     7561 tgaaagttat caggatccgc tgaacaaaca gtatccgctg cagcttacgg gtttccacta
     7621 taaatctcgc gttcactcaa cttacggcaa cgttgatgtg ctgaaagcag cttgccgtca
     7681 ggaaatgtgg atcaacccgc ttgatgcgca aaaacgcggt atcagcaacg gcgataaagt
     7741 caggatcttt aatgatcgtg gtgaggttca tattgaggcg aaagtgacgc cacgaatgat
     7801 gccgggtgtg gtcgcactgg gtgaaggtgc ctggtatgac ccggatacaa aacgtgtcga
     7861 taagggcggt tgtattaacg tactgaccac tcaacgtccg tctcctcttg ccaaggggaa
     7921 tccatcacat acgaaccttg ttcaggttga aaaggtgtaa ggagtaaccg atgacaaccc
     7981 agtatggatt ttttattgat tccagccgtt gcaccggttg caaaacctgc gagctggctt
     8041 gtaaagacta caaagatttg acgccagaag tcagcttccg ccgcatttat gaatatgctg
     8101 gtggcgactg gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc tactatctgt
     8161 cgatttcatg taaccactgc gaagatccgg cttgtactaa agtctgcccg agcggtgcga
     8221 tgcataaacg tgaagatggt tttgttgtgg tcgatgaaga tgtatgcatt ggctgccgtt
     8281 actgtcatat ggcttgcccg tatggcgcac cgcaatataa cgaaacgaaa ggccatatga
     8341 ccaaatgcga tggttgttat gaccgtgttg cggagggaaa aaaaccgatc tgcgtagagt
     8401 cctgccccct gcgggcgctg gatttcggcc ctatcgacga gctgcgtaaa aaacatggcg
     8461 atctggcggc cgttgcgccg ttgccgcgag ctcactttac caaaccgaat attgtgatca
     8521 aacccaatgc caacagccgc ccaaccgggg ataccactgg ctatctggca aacccgaagg
     8581 aggtgtgaga tgggaagtgg atggcatgaa tggccgctga tgatcttcac ggtcttcggg
     8641 caatgtgtag cgggtggttt tatcgttctg gctttggcgc tgctcaaagg cgacctgcga
     8701 gcagaagccc agcagcgtgt tatcgcctgc atgttgggtt tatgggtgct gatgggcatt
     8761 ggctttatcg cctctatgct tcatcttggt tcaccgatgc gcgcttttaa ctcgctcaat
     8821 cgggtagggg cttcagcact cagtaacgaa attgccagcg gttcgatctt ttttgctgta
     8881 ggcggcatcg gctggttgct ggcaatgctg aaaaagcttt caccggcatt gcgtacgctt
     8941 tggctggtgg tgacgatggt tcttggcgta gtctttgtct ggatgatggt gcgtgtgtat
     9001 aacagcatcg atacagtacc cacctggtac agcatctgga caccgatggg cttcttcctg
     9061 acgatgttta tgggcggccc gttactgggt tacctgttgc tgagtctggc tggtgtcgat
     9121 ggctgggcga tgcgtctgct gccagccatt tctgttctgg cactggtagt gagtggcgtg
     9181 atgtcagtga tgcagggcgc agagctggcg aatattcata gctctgtgca gcaggccgca
     9241 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
     9301 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactttct
     9361 gtatcattca ttctgcttct ggcaggggaa ttaatcggtc gtggcgtatt ctatggtttg
     9421 catatgaccg tggggatggc tgtcgcaagt taatcagtaa caaccggggt ttcggccccg
     9481 gttcgctttt atttctgctt cgttaacgtg tcataactgg tcatcaagtt acgataatca
     9541 ggaatgtggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcgcgatgc
     9601 agctcgcacg ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
     9661 catgccgaat gccgggtaat ttcattaaag gtccctgacg cgtcagtcac gacaaagacg
     9721 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
     9781 ccggcaataa ttaactgttt tttacctgtc gctttgacag cttttacaaa atcttcgtta
     9841 tcccaggcgt taatatttcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
     9901 agttctggaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
     9961 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    10021 tcgatatctc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    10081 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    10141 gtacgtctaa atcggtcaga cgtgatgcat tgctctgaaa gcatagacgg gaaatatgag
    10201 tttgctgtga ccatgaaatt tttcgactga acgaagcgta tgaaatttgt gttagatcaa
    10261 taaaaacaat cagatgagat taatcgtctc ttcagcatta gcgttatgaa tgagtaatat
    10321 aaatcgataa taccgccgct gcgatatgcg cgtattttca cccattgaca atgtttttgg
    10381 cggtggcatg atgcgcatga aatttgaact tcctcacggt tttaattcat gtccacgtat
    10441 acccggcctg tcatgctttt gctgtctggc ctgcttttgt tgactctggc gattgcggtg
    10501 ttaaatacac tcgtgccgct ttggctcgcc caggaacaca tgtctacatg gcaggtaggc
    10561 gttgtcagct catcctattt taccggcaac cttgtcggta cattgctgac agggtatgtc
    10621 attaaacgca ttggctttaa ccgtagctat tatctggcct ccttcatttt tgccgctggc
    10681 tgtgccggcc ttggcctgat gattggattc tggagttggt tggcgtggcg ttttgtcgcg
    10741 ggcatcggct gtgccatgat ttgggtggtt gttgagagtg cgctgatgtg cagtgggacg
    10801 tcgcgtaacc gcgggcggtt gcttgctgcg tatatgatgg tttattacgt cggaacgttt
    10861 ttaggccagt tactggtcag caaagtctca accgagttga tgagtgtatt gccgtgggtt
    10921 acaggtttga cgttggcagg aatcttaccg ctgttgttta cgcatgtcct gaatcagcag
    10981 gctgaaaacc atgattcgac gtcaattacg tcaatgctaa aactccgtca ggcgcggctt
    11041 ggcgtgaatg gctgcattat ctcaggaatc gtgctgggat ctctatatgg cctgatgccg
    11101 ctgtacctca atcacaaagg ggtgagcaat gccagcattg gtttctggat ggcggtactg
    11161 gtcagtgcgg gtatcctcgg gcaatggccg attggacgtc tggcggataa gtttggtcga
    11221 ctgctggtgt tgcgtgttca ggtctttgtc gtcattctcg gcagtattgc gatgcttagc
    11281 caggcggcga tggccccagc gttattcatc ctcggtgccg ctggctttac gctatatccg
    11341 gtggcgatgg cctgggcttg tgagaaagtt gaacatcatc aactggtagc gatgaaccag
    11401 gccttactgt tgagctatac cgtgggaagc ctgcttggcc cgtcatttac cgctatgcta
    11461 atgcagaatt tctccgataa tttattattt atcatgatcg ccagcgtatc gtttatctat
    11521 ttgctgatgc tgcttcgtaa cgccggtcat acgccgaaac ccgtggctca cgtgtaaatg
    11581 aattcaagca gagtgtgaac ttactgtttc acactctgct tttctgtgca caaaaaagcg
    11641 gcaacttaat aaagttgccg ctttacgggg aaattagaac atgaccttat gaccgtactg
    11701 ctcaagaatg cctttcacgc gttccatggt ctctttcttc ggtggtttaa caccatcgag
    11761 tttgtattct tcacccattg ccacccattt gtgtttgccc agttcgtggt aggggaggag
    11821 ctcgattttc tcaacgttgc ccatatcacg ggtaaattca ccaaggcgat gcgctgaatc
    11881 gtcatcgtca gaccagcctg ggacaacaac atagcggatc cacaccttca catttttgtt
    11941 cgccagatat ttagcgaact ccagcgtgcg gtggttggaa actccaacca gattttggtg
    12001 gatctcgtcg ttcatctgtt tgagatcgag cattaccagg tcggttactt ccagcagttc
    12061 atcaatcacc ggatcgtaac gacgaacaaa accgttggtg tccagacagg tatgaatgcc
    12121 ttcttttttg caggcgcgga accagtcacg aacaaactca gcttgtagga ttgcctcacc
    12181 gccggatgcg gtaacgccgc cgccggaagc gttcataaag tggcgatagg tcaccacttc
    12241 cttcatcaaa tcttcaacgg taacttcttt accgccatgc gtatcccagg tgtcgcggtt
    12301 atgacaatac aggcagcgca tcaggcagcc ctggaaaaag gtgataaagc gaatacccgg
    12361 gccgtctacg gttccacagg attcaaagga gtgaatgcga ccaataactg acattgcggt
    12421 gtttctccag atgtggccca tctgaggccg tgttggtgcg cagctcgaag gctacgtcga
    12481 gtctgttttg gcagtcacct taaagtatag atagctgaca aaaaaggctc tcgtgctaaa
    12541 aaaggcccca ctttcgtgga gcctttattg tacgcttttt actgtacgat ttcagtcaaa
    12601 tctaattaca tagattgagt gaaggtacga gtaataacgt cctgctgctg ttctttagtc
    12661 agcgagttga aacgtactgc gtagccagat acacggatgg tcagctgcgg atatttttcc
    12721 gggttttcca tcgcgtcgag cagcatttca cggttcatca cgttaacgtt caggtgctga
    12781 ccaccttcga tggatgcttc gtggtggaag taaccatcca tcagaccagc caggttggtc
    12841 ttacgaactt cgtcgtcttt acccagtgcg ttcggaacga tagagaaggt gtaggagata
    12901 ccatctttag cgtaagcaaa cggcagttta gcaacggaag tcagagacgc tacagcacct
    12961 ttctggtcac gaccgtgcat cgggttagca cccggtccga acggcgcgcc agcacgacga
    13021 ccgtctgggg tgttaccagt tttcttacca tacacaacgt tagaagtgat ggtcagaaca
    13081 gactgagtcg ggatagcgtc acggtaggtg tgcagtttct gaattttctt catgaaacgt
    13141 tctaccaggt caacagccag gtcatctaca cgcggatcgt tgttaccaaa ctgcgggtat
    13201 tcgccttcga tttcgaagtc gatggccaga ccgtcttcgt cacgaatcgg tttaactttc
    13261 gcatatttga ttgcagacag ggagtcagca gcaacggaca gaccagcgat accacacgcc
    13321 atggtgcgga taacgtcacg gtcgtgcagc gccatcagag aggcttcgta gctgtacttg
    13381 tcgtgcatgt agtggatgat gttcagtgca gtgatgtact gtttagccag ccagtccatg
    13441 aagtgatcca tgcgctccat cacttcatca tagttcagga catcgccttt gatcggttca
    13501 gacttcggac caacctgcat tttcagtttt tcgtcaacgc cgccgttgat tgcgtacagc
    13561 atggttttcg ccaggtttgc acgcgcaccg aagaactgca tttgtttacc aacgatcatc
    13621 gggcttacgc agcaagcaat agcgtagtca tcgttgttga agtccggacg catcaggtca
    13681 tcgttctcat actgcagaga agaggtgtcg atggacactt tagcggcgaa tttcttgaag
    13741 ttcagcggca gtttttcaga ccacagaatg gtcatgttcg gttccggaga cggacccatg
    13801 gtgtacaggg tgttcaggaa acggaagctg tttttggtaa ccagggtacg accgtcgagg
    13861 cccataccac cgatagattc ggttgcccag atcgggtcgc cagagaacag ttcatcgtat
    13921 tccggagtac gcaggaagcg aaccatacgc agtttcatga ccaggtggtc aaccatttcc
    13981 tgcgcttctt gttcggtgat cttgccagct ttcaggtcac gttcgatgta cacatccagg
    14041 aaggtggagg tacgaccgaa ggacattgca gcaccgttct gagacttaac agcagccagg
    14101 tagccgaagt aagtccactg gatagcttcc tgagcgttgg tagccggacc agagatgtcg
    14161 tagccgtatt tcgcagccat ttctttcatc tgacccagag cgcggtgctg ttcagcgatt
    14221 tcttcgcgca gacggatagt ctgttccagg tttacgccgt tttccagatc agcctgcaga
    14281 gaggtgaact gtgcaagttt gtctttcatc aggtagtcga taccgtacag cgcaacgcga
    14341 cggtagtcac cgatgatacg accacggcca taagcatctg gcagaccggt cagaacaccg
    14401 gatttacggc aacgcaggat gtccggagtg taaacgtcga acacgccctg gttgtgagtt
    14461 ttacggtatt cagtgaagat ttttttgatc atcgggtcca gttcgcggtt gtacgctttg
    14521 caggaacctt cgatcatttt gataccaccg aacgggataa gagcacgttt cagcggagct
    14581 tcagtctgca gaccaacgat tttctcaagc tgcttgttga tgtaaccagc gtcgtgagag
    14641 gtgatggtgg aagcaacagc ggtgtcaaag tcaactggcg catgagtgcg gttttccagt
    14701 ttaacgcctt ccattacttt gtcccacagg gtggtggtcg cttcagtagc gccagccagg
    14761 aaggactcgt caccctcgta cggagtgtag tttttctgaa tgaagtcacg gacgtttact
    14821 tcattctgcc agtcaccttt ggtaaaacct tcccaggctg tggctaactt ttcattaagc
    14881 tcggacatgt aacacctacc ttcttaagtg gattttttat ttactgcgta cttcgacaac
    14941 cattaatggt gatcgttttc acgcaggtaa atgacccagt atgtcaaccc aaccaacaaa
    15001 ccaccgccga taatattacc gatcgtaacc ggaatcaggt tatcagtgat gaaattcatc
    15061 acggtcaggt gagaaaaatt ttccggtgca gaaccgacag cggtccagaa ttccggagat
    15121 gcgaagtcgc ggattacaat acccatcggg atcataaaca tgtttgcgat actgtgctca
    15181 aaaccgctgg caacaaacat cgcgaccggc agcaccataa tgaacgcttt gtccatcagg
    15241 ctgcggccag aataactcat ccatactgcc agacatacca tcaggttcgc caggatacca
    15301 agacagacgg cctcaataaa agtatggtgc actttgtggt cggcggtttg taggacgttt
    15361 agtccccatt gaccatttgc ggtcatatac tcgccggaaa gccacattaa aagtacaaac
    15421 agcagtgcgc cgaccaggtt gccaaaataa acatttagcc agtttttcgc caactgaccc
    15481 caggtgatgc gcccactcgc cttggcaaca acaatcaaca ctgtggaagt aaagagatcg
    15541 gctccgcaga caacacaaag aatcaacccc agagagaagc aaatgccgcc aaccagtttt
    15601 gccatgccga agggcattgt acctgtgcca gtggttgctg tgatatagaa gacgaatgcg
    15661 attgagatga aaacaccggc ggtaatcgcc agatagaaag tcttaagcgg atgtttcgtt
    15721 gctttataga cacctgcctc ttcggccact ttggccattg cagcaggaag taaaagatca
    15781 aaagggttgt cagctttcac actaactctc tctttattaa gtcggcgacg agatactaac
    15841 aaagcattat agatgagaaa ttgatataga tcatatctcg cctggcttat aggcccgtaa
//
LOCUS       DADTFG010000114        15859 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.115, whole genome shotgun sequence.
ACCESSION   DADTFG010000114 DADTFG010000000
VERSION     DADTFG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.115"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(66..791)
                     /gene="phoU"
                     /locus_tag="J1138_003366"
     CDS             complement(66..791)
                     /gene="phoU"
                     /locus_tag="J1138_003366"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBA8455128.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(806..1579)
                     /gene="pstB"
                     /locus_tag="J1138_003367"
     CDS             complement(806..1579)
                     /gene="pstB"
                     /locus_tag="J1138_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBA8455129.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1670..2560)
                     /gene="pstA"
                     /locus_tag="J1138_003368"
     CDS             complement(1670..2560)
                     /gene="pstA"
                     /locus_tag="J1138_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBA8455130.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2560..3519)
                     /gene="pstC"
                     /locus_tag="J1138_003369"
     CDS             complement(2560..3519)
                     /gene="pstC"
                     /locus_tag="J1138_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBA8455131.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3606..4646)
                     /gene="pstS"
                     /locus_tag="J1138_003370"
     CDS             complement(3606..4646)
                     /gene="pstS"
                     /locus_tag="J1138_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBA8455132.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4960..6789)
                     /gene="glmS"
                     /locus_tag="J1138_003371"
     CDS             complement(4960..6789)
                     /gene="glmS"
                     /locus_tag="J1138_003371"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBA8455133.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6951..8321)
                     /gene="glmU"
                     /locus_tag="J1138_003372"
     CDS             complement(6951..8321)
                     /gene="glmU"
                     /locus_tag="J1138_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBA8455134.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQHLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQADKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPIKKK"
     gene            complement(8672..9091)
                     /gene="atpC"
                     /locus_tag="J1138_003373"
     CDS             complement(8672..9091)
                     /gene="atpC"
                     /locus_tag="J1138_003373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBA8455135.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9112..10494)
                     /gene="atpD"
                     /locus_tag="J1138_003374"
     CDS             complement(9112..10494)
                     /gene="atpD"
                     /locus_tag="J1138_003374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBA8455136.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10521..11384)
                     /gene="atpG"
                     /locus_tag="J1138_003375"
     CDS             complement(10521..11384)
                     /gene="atpG"
                     /locus_tag="J1138_003375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBA8455137.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11435..12976)
                     /gene="atpA"
                     /locus_tag="J1138_003376"
     CDS             complement(11435..12976)
                     /gene="atpA"
                     /locus_tag="J1138_003376"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBA8455138.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12989..13522)
                     /gene="atpH"
                     /locus_tag="J1138_003377"
     CDS             complement(12989..13522)
                     /gene="atpH"
                     /locus_tag="J1138_003377"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBA8455139.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13537..14007)
                     /gene="atpF"
                     /locus_tag="J1138_003378"
     CDS             complement(13537..14007)
                     /gene="atpF"
                     /locus_tag="J1138_003378"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBA8455140.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14069..14308)
                     /gene="atpE"
                     /locus_tag="J1138_003379"
     CDS             complement(14069..14308)
                     /gene="atpE"
                     /locus_tag="J1138_003379"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBA8455141.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14355..15170)
                     /gene="atpB"
                     /locus_tag="J1138_003380"
     CDS             complement(14355..15170)
                     /gene="atpB"
                     /locus_tag="J1138_003380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBA8455142.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15179..15559)
                     /gene="atpI"
                     /locus_tag="J1138_003381"
     CDS             complement(15179..15559)
                     /gene="atpI"
                     /locus_tag="J1138_003381"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBA8455143.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3806 a         4303 c         3966 g         3784 t
ORIGIN      
        1 taggatttta taaagttata tataacaaat cccaataatt gaattattgg gatttacttg
       61 gtgaattatt tatcgctatc tttttctgcc agcagtttat cgagctcgtc accaccgaca
      121 tgacggaaat cctgcccctt cacgtagtag aagataaact cacaaatatt ctggcagcgg
      181 tcgccaatac gttcgataga acgcgcacag aataacgcgg taagtacgct cggaatggta
      241 cgcgaatctt ccatcatgta ggtcatcagt tgacgaacaa taccttcgta ctcctgatcg
      301 acttttttat cttcacgata aatacgtacc gcttcgtcaa tgtccatccg cgcgaacgcg
      361 tccagcacgt cgtgcagcat ctggatggta tgacggccca gcgactccag acttaccagc
      421 aatggctgat gctgctggga gaatttctcc agtgcagtac ggcagatttt gtccgccacg
      481 tcgccaatac gctccagctc ggcaatggtt ttactgatca ccataaccag acgcaggtcg
      541 ctcgccgtcg gctgacgttt ggcgataatg cgcacgcagg cttcatcgat cgccacttcc
      601 atcatgttga cgttcttgtc gccttcgatg acgcgcttcg ccagatcgct gtcctggtta
      661 tgcatcgcgg tgattgcatc agaaagctgc tgctccacca tgccgcccat ggtcatcacc
      721 tgcgtgcgga tgctttccag ttcggcgttg aactggccgg aaatatgttt attaagattg
      781 agactgtcca taacgcactc ctgaatcaac cgtaacgacc ggtgatgtag tcttctgttt
      841 gtttcttcgc tggcttggtg aacagatcgt ccgtgttgct gaactcaatc aattcgccca
      901 ggtacataaa cgccgtgtgg tcggaacaac gcgcagcctg ctgcatgttg tgggtgacga
      961 tcaccacggt gtaatcctgc ttcagttcgg tgatcagctc ttcaatacgc ccggtagaga
     1021 tagggtcgag tgccgaacac ggttcgtcga gcagcagcac ttccgggcga atggcgatac
     1081 cacgcgcaat gcacagacgc tgttgctgac caccagagag agagtaaccg ctctggtgca
     1141 atttatcttt ggtttcgttc cacaatgcgg ctttggtcaa tgcccactgc acgcgctcgt
     1201 ccatgtcagc acgggagagc ttctcaaaca gacgcacgcc aaaagcgatg ttgtcgtaga
     1261 tggacattgg gaacggcgtc ggtttctgga acaccatgcc cactttcgca cgcagcaggg
     1321 cgatatcctg agagttggtc aggatgttgt cgccatcgag cagaatttca ccttccgcac
     1381 gctgttccgg gtacagttca aacattttgt tgaaggtacg cagcagcgtt gatttaccgc
     1441 agccggacgg cccgataaac gccgtaacct ggtttttggc gatatccagg ttgatgtttt
     1501 tcagggcatg gaatttgccg tagtagaagt tcaaattacg aacctgaatt ttactcgggg
     1561 cagtttcaac catactcatt gcaatctttt cctcatcgtg cctgatgcac ttcgcgaatc
     1621 aggcttaccg taggccggat aaggcgttta cgccgcatcc agcaaagtgt taaccgtgtt
     1681 tattcttcgc aaaaacaacg cgcgccagaa tgttcagcag cagtacgcac agggtaatga
     1741 tcaacacccc ggcccaggcc agttgctgcc attccgcaaa cgggctcatc gcaaacttaa
     1801 agatcgtcac cggcaggttg gcgatcggct gcatcatgtc cgtgctccag aactggttgg
     1861 agagcgcagt aaacagcagc ggcgcggttt caccagcaat acgtgcaatc gccagcagga
     1921 taccggtcat gatcccggac accgacgctt tcagcgtgat cgaggcgatc atcttccact
     1981 tcggcgtacc cagcgcataa gccgcttcac gcaggctgtc cggcaccagt ttcagcatgt
     2041 tctcggtggt gcggataaca atcggcacct gcaacagcgc cagcgcaatc acgcccgccc
     2101 agccggagaa gtgctccatc tgcgccacca caatggtgta aacaaacaga ccaaccacaa
     2161 tcgacggcgc agagagcaga atgtcgttaa tgaagcgaat cacttctgcc agccaggatt
     2221 tacgaccata ttcagccagg taaatccccg ccataatgcc cagcggcgta ccgaatacgg
     2281 tagcccacaa aattaacagg ccgctacccg ccagggcgtt cgccagacca ccgccttccg
     2341 tattgggtgg tggcgtcatt tcggtgaaca gcgccagcga cataccgtcg ataccgcgag
     2401 tgatggtgga cattaaaatc cagatcagcc agaacagacc gaaggccatc gtcgccattg
     2461 agagcgtcag cgcaatacgg tttttgaggc ggcgacgcgc ctgcattttg cggcgggatt
     2521 cagccagtgc cgcagtggtt tgcatttcaa ccatagccat tagcgtgccc cctcattctt
     2581 agccaggcgc ataatcataa acttcgatgc ggcgaggacg atgaaggtaa tcacaaacag
     2641 gatcaggccc agttccatca gtgcagcgac gtgcagaccg gattccgctt ccgcaaattc
     2701 gttggccagc gcagaggtga tactgttgcc cggcatatac agcgaggcgc tgtcgagttg
     2761 gtaggtgtta ccgatgataa aggtcaccgc catggtttca ccgagcgcgc gccccagccc
     2821 caacataatg ccgccgataa caccattttt ggtgaacgga agaacgatac gccagataac
     2881 ttcccaggtg gtgcagccaa taccgtaggc cgactctttc atcatcaccg gggtttgttc
     2941 gaatacatcg cgcattaccg ctgcaatgta cggaataatc atgatggcga ggatcacgcc
     3001 tgccgcgagg ataccgatac caaaggccgg gccggagaac agcgcgccga caatcgggat
     3061 attcgacatg atattgccga ccggctcctg gaagtaaacg gcgaacagcg gcgcaaagat
     3121 aaacaggccc cacatgccgt aaacgatact tggaatggca gccagcagct caatggcgat
     3181 acccagcggg cgtttcagcc agccaggcgc aagctcggtc aggaacaggg cgataccaaa
     3241 actcaccggg acggcgatca gcagcgcgat aaacgaggtc accagcgtac cgtagatcgg
     3301 caccagcgcc ccgtagatat cgttcggcgc atcccactct ttggtccaca ggaaagccag
     3361 accaaatttc tgaatgctcg gccaggatga gatgatcaga gagacaataa tgccacccaa
     3421 catcaatagc acaatcagcg ccgccagttt taccagcacg ctgaaaatta tgtcgccctt
     3481 tttacccggt gggttaaaag caggcttggt tgcagccata agttactctt cagttaaacg
     3541 cgtttacaaa gtcattttgt aatgccggat gcggcgtaaa acaccgtacc cggcctggag
     3601 ttttattagt acagcggctt gccgctactg tctttaatat tggtcttcca cgcagcgcga
     3661 acctgttcaa ctacgctatc cggcaggctg gcataatcca ggtcgttcgc ctgtttagcc
     3721 ccggttttgt atgcccagtc gaagaacttc agcacttcgg tgccttgttc tggtttcttc
     3781 tgatctttat ggatcagaat gaacgtggta gaggtaatag gccatgcatc ttcgcctttc
     3841 tggttggtca gatcctgagc gaatgttttg ctccagtctg cgccttttgc tgcattagcg
     3901 aagttttctt cggtcggact caccggtttg ccatcagcgg agatcagttt ggtgtacgcc
     3961 aggttgttct gcttcgcgta agcatattca acgtaaccaa ttgcacccgg cagacgctga
     4021 acgaacgcgg cgataccgtc gttaccttta ccgcccagac cgatcggcca ttttacggta
     4081 gagccagtac caacgttgtt tttccactct tcgttcactt tcgccaggta gctggtgaag
     4141 acgaaggaag tcccggagcc atctgcgcgg cgtactacag caatgttctg tgaaggcagt
     4201 ttcagacccg gattcagttt ggcgatggct tcatcatccc acttcttgat tttgcccagg
     4261 tagatgtcgc cgagggtttt accatccagc accagttcgc cagacttcag acctggaata
     4321 ttaaccgcca gcaccacgcc gccaatcacg gtcgggaact ggaacaggcc ttcctgagcc
     4381 agtttttcgt cagataacgg cgcatcagaa gcaccgaaat caacggtatt agcgataatc
     4441 tgttttacgc caccggaaga accgataccc tggtagttaa ctttattacc ggtttctttc
     4501 tggtaagtgt cagcccattt ggcatacacc ggcgcaggga aggttgcacc tgcacctgtc
     4561 aggcttgctt ctgcaaacac agagaaagca ctcatcgata aggtcgcggc gacaacagtt
     4621 gcgacggtgg tacgcataac tttcataatg tctcctggga ggattcataa agcattgttt
     4681 gttggctacg agaagcaaaa taggacaaac aggtgacagt tatatgtaag gtttatgaca
     4741 gttttatgac agagagagaa agtctttagt ctgatttaaa taagcgttga tattcagtta
     4801 attacaaata ttaataacga agagatgaca gaaaaatttt cattctgtga cagagaaaaa
     4861 gtagccgaag atgacggttt gtcacatgga gttggcagga tgtttgatta aaaacataac
     4921 aggaagaaaa atgccccgct tacgcagggc atccatttat tactcaaccg ttaccgattt
     4981 tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcaacgt ggtaagccag
     5041 tagttgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat gcggcatctc
     5101 aataatgtgc atattgtcac tgctcacaaa acccgcatcc tgatcggcga agacatacaa
     5161 ctgaccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca gcaattcgtt
     5221 gttcggtgca acgacgataa ccggcatatc ggcatcaatc agcgccagcg gaccgtgttt
     5281 cagttcgcct gcagcgtagg cttcagcgtg aatgtaagag atctctttca gcttcaatgc
     5341 accttccagc gcgattgggt actgatcgcc acggcccagg aacagcgcgt gatgtttgtc
     5401 agagaaatct tctgccagcg cttcaatgcg tttgtcctga gacagcatct gctcaatacg
     5461 gctcggcagc gcctgcagac catgcacgat gtcatgttca atggaggcat ccagaccttt
     5521 caggcgagac agtttcgcca ccagcatcaa cagcacagtt aactgggtgg tgaatgcttt
     5581 ggtggaggcc acgccaattt ccgtaccggc gttggtcatc agcgccagat cggattcgcg
     5641 caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac ccagctcttt
     5701 cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca aggtgatcat
     5761 caggctgtta cgacgcacgg cagatttgcg atagcggaat tcagaggcga tttcgacgtc
     5821 gcacggaata cctgccagcg attcaaacca gtagcgggaa accataccgg agttataaga
     5881 agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcat cggcgttcgg
     5941 tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg tgtttttgat
     6001 cgcgttcggc tgttcgtaga tctctttctg catgtagtgg cggtaaatac ctttatcgcc
     6061 cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc cagttttatc
     6121 gaagatgttt accgaacggc gagtgatttc cgcaatatcg ccctcttcaa ggaagataaa
     6181 gcgacgagtc actggcaaca gcgccagctg gtcagaagcg ataaagtttt cgcccatacc
     6241 caggccaatc accagcggac taccagaacg tgccgccagc agggtatccg gatgacggga
     6301 gtccatgatc actgtaccgt acgcaccacg cagttgcggg atagcacgca gaactgcctc
     6361 acgcagagta ccgccttgtt tcagctccca gttcaccaaa tgtgcaatca cttcggtgtc
     6421 ggtttcagaa acgaaggtat agccacgcgc ttttaactct tcacgcagcg gttcatggtt
     6481 ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat gcgcgttagc
     6541 ttctgaaggt tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc cgccatgcag
     6601 aggatgttct tccgctgcct gagccagcat ctgtacttta ccgaggcgac gcaggcgggt
     6661 catatgacct tcagcatcca ccacggccag gccggcagag tcatatccgc gatattccag
     6721 acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg cgccaacaat
     6781 tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta tgcccgtttt
     6841 ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg cttgtgggcg
     6901 ggagattatg ttatctcctc atcccatatg accggattac ccggccagaa ttactttttc
     6961 tttatcggac gacgccagcc ttctttttga gtctgcggca cacggctgat agccagggca
     7021 ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc tttgcctact
     7081 gttaccgggg ccaccagttg agtgtcggaa ccgacaaaca catcgtcgcc gataatggtc
     7141 ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcaccgat gttaacgtta
     7201 tcgccaattt ccgcatcgcc caggtaagtc agatgaccag ctttcgagcc tttacccaga
     7261 cgcgcttttt tcatctcaac gaagttaccg acgtgtgcac cttccagcaa ctcagcacca
     7321 ggacgcagac gggcaaacgg gccaatagta caggccgctg ccagattcgc atcttccacg
     7381 acggtatatg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat cacgcaaccg
     7441 gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac gttagtatca
     7501 atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc tggatcgcgc
     7561 agcataacgc ctgctaacag cagtttatca gcctgttcgg actggtaaac gcgctccagt
     7621 cgggagagtt gcaggcggtt attcacgcct tctacttcgc ttaaacgttg cggatgaacg
     7681 gcgacgattt cacgtccttc ctgatacgcc agcgcaataa tgtcggtgat gtagtattcg
     7741 ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc gccgttggca
     7801 atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc atctttgtgc
     7861 tcaacaatgc cggtaacttt gccgttttca cgggtgatac gtccataacc ggtcggatca
     7921 tccagtttta ccgtcagcag accaatgcca ccttgcggtt tagcatcacg caggtgctgg
     7981 agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc ttcatcatcg
     8041 gcaaagaaag gtgcggcctg ctgcatcgca tgacccgtac ccagctgttc tgcctgaagc
     8101 acccagttca ggttgtcgtc tttcagcgcc tgcttaagca gatcgccgcc gtgaccatac
     8161 acaaggtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg ctgaaccatc
     8221 gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg cgtgcctttg
     8281 cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct gactgtaatt
     8341 tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat cgtgaaatgg
     8401 acagaggata aattgttcaa taacggatta tccccgctga cgaaagcgcc attttcgacc
     8461 atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag cgtcaggtgg
     8521 atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acagggtgtt ttatatcagc
     8581 aggatctatg tgaacgctct tcaggacggg tcaacgcgta aaaaaaagcc agcctgttac
     8641 cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc aactcgataa
     8701 cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa tctacgtcgc
     8761 cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct cgcgcttcgt
     8821 cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca ggctgcactt
     8881 caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc acgatgcgaa
     8941 tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc cccagttcac
     9001 cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt tgctctgcgc
     9061 tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc gttaaagttt
     9121 tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct gctccggcag
     9181 gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt tcagggagac
     9241 gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg acaggaagcg
     9301 ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt catccatacc
     9361 caggatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact gaacgccacg
     9421 cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc tggtggagtc
     9481 cagcgggtca acggccgggt agatacccag agatgcgatc tgacggctca gtaccacggt
     9541 tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat ccgcaggtac
     9601 gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac gttcctgcag
     9661 aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca tacggcccag
     9721 cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa cgaacagcag
     9781 aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca gagcaacgcg
     9841 cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt tgtcgataac
     9901 gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt cacctacgcc
     9961 cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaataagct ccatcatgtt
    10021 tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct tagcgaacgg
    10081 acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt ttgacagctc
    10141 ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga tctcgccttt
    10201 catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt tacctaccgg
    10261 gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac cgtcggagga
    10321 acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt ccagcaccag
    10381 acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg catcctgagg
    10441 gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag ccatcttaaa
    10501 tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc ggtgagttcc
    10561 tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt aatcaggctg
    10621 ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc gctggccagg
    10681 ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag ggtatccagc
    10741 aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tgtgtttcag atcatcatca
    10801 tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat ggtgttaata
    10861 aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaggc ctgcaacatc
    10921 acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc ggtgacctgg
    10981 gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc gatcattgcg
    11041 aggtcgcatt gaacgccttt gtcggtccag gtcttcattt ccgccagcag ttttttgaac
    11101 aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag gtagcccacg
    11161 cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc gtgtgcaagg
    11221 tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat gcgatcctgc
    11281 gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt ttgcgtgttc
    11341 tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt ctcctcaatg
    11401 ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt gctttgaagg
    11461 aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg gtctggttga
    11521 tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg gcttcgaagc
    11581 tgccgatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg aacagaacca
    11641 gagactgttg cgctacggac atcggcgcat actgtttctg tttcagcagt tcggtcactt
    11701 tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca aactgagaga
    11761 acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac agttttttca
    11821 tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg ttaaccgcag
    11881 gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg gtaatggaga
    11941 ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata atcggcagcg
    12001 cagtcagaga accggttttc cctttcactt cacctttggt gaaagcttca acgtattcgg
    12061 cgttaacacg cgcagcacgc tccagcagac gagagtggag gtagaatacg tcgcccggga
    12121 atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa gcaacagcct
    12181 gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg aagtattcac
    12241 ccattgcgca accagcgtac ggtgccaggt attgcagtgc tgcggattca gacgcggttg
    12301 ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt accacgttag
    12361 aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg gaatcgcgct
    12421 ggttgatgat ggcatcgata gccagtgcgg ttttaccggt ctgacggtca ccgatgatca
    12481 attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa ccggtctgta
    12541 ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct acagcagaga
    12601 agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg ttaaccacac
    12661 ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac ttaactttca
    12721 tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag tcgcgctcga
    12781 ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc tgcatacaat
    12841 cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta ccttcgttgt
    12901 gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg ctgatttcgg
    12961 tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca aggcgctcaa
    13021 gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata acgcctgcca
    13081 ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt ttttccatcg
    13141 cagcagaaat tttcgcgagc tgttgttcac tcaacgcggc agcggaaatg acgtctacct
    13201 cagcggtagc ctcactcaca gcacgcaggt gaataaactg ctccagaaca tccgggagcg
    13261 cgttaagacg accattttcg gccattaccc gaatcaggtt ctgaccgttt tcgtccagtt
    13321 gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc gcgccagaga
    13381 gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc atgtcctgcc
    13441 agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg taggggcgag
    13501 ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga caagtttatc
    13561 cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct cggcgccagc
    13621 aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac gctcggcttc
    13681 aatttccgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct cagctttcgc
    13741 ttcgtccaga atctgcgagc ggcgtttgtt agcctgctcg atgattacct gggcttccgc
    13801 tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt ccttatgtgc
    13861 tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga tggctgccat
    13921 taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg cgatggcctg
    13981 gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta acgttctgat
    14041 attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg tacagaccca
    14101 gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag aactgagtac
    14161 gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta cccccgagga
    14221 tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg gcagccatgt
    14281 acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag ttaaaacgta
    14341 gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat cgtcagaacc
    14401 atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca cggcacattc
    14461 aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat cagctcaccg
    14521 gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag caggcttacc
    14581 ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg cagcgtcaac
    14641 tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat caggataaat
    14701 acgcccagtg ccatagacag cgttacgttc acgtcagcag acggaaccac acgcagtgca
    14761 ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag taaatccatc
    14821 aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat cagcttgctt
    14881 ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag ctcaatcgcg
    14941 gtctgaaact tacctggcac gccgctggtt gcctttttgg ctacactacg gaataaaacc
    15001 aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat tgtccagaag
    15061 gtggctgggg ggttatgtgg atccaccagc gagaatgtac gcaggtccag ctgaaggtta
    15121 ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat gatgcctttt
    15181 accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc aaaacccacg
    15241 taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc agtaacacca
    15301 acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc cggcctttcg
    15361 ctggtgtatg cgcctggtga cgccaggcaa atatcataaa caaaacgtta ggcagaaaga
    15421 ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg ctgaacagca
    15481 atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca acgtttcgac
    15541 tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta tgccgcgtgt
    15601 cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa attatacggt
    15661 gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt aaatattttt
    15721 ccagtgcata caattgcgac ttttctgcaa accctgttcg atcatgaaaa actgtaaata
    15781 acgcgtaaac actggtgata aagcgtgctt cagatcacat attgcgcatg ttcgcgcaca
    15841 gcatatttat ttacttggc
//
LOCUS       DADTFG010000115        15716 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.116, whole genome shotgun sequence.
ACCESSION   DADTFG010000115 DADTFG010000000
VERSION     DADTFG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.116"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            18..878
                     /locus_tag="J1138_003382"
     CDS             18..878
                     /locus_tag="J1138_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8455144.1"
                     /translation="MEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFD
                     RVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILPAVIA
                     RYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDELVVFA
                     APTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIK
                     HAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFL
                     DYCDPANVPR"
     gene            1083..2552
                     /gene="lysP"
                     /locus_tag="J1138_003383"
     CDS             1083..2552
                     /gene="lysP"
                     /locus_tag="J1138_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBA8455145.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2844..4835
                     /gene="cirA"
                     /locus_tag="J1138_003384"
                     /pseudo
     CDS             2844..4835
                     /gene="cirA"
                     /locus_tag="J1138_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(4867..5703)
                     /locus_tag="J1138_003385"
     CDS             complement(4867..5703)
                     /locus_tag="J1138_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HBA8455146.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5961..6629
                     /gene="folE"
                     /locus_tag="J1138_003386"
     CDS             5961..6629
                     /gene="folE"
                     /locus_tag="J1138_003386"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBA8455147.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6646..7803
                     /locus_tag="J1138_003387"
     CDS             6646..7803
                     /locus_tag="J1138_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBA8455148.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGGISDSQTSRA
                     WTPDTSAILYEKHWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            7945..8985
                     /gene="galS"
                     /locus_tag="J1138_003388"
     CDS             7945..8985
                     /gene="galS"
                     /locus_tag="J1138_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBA8455149.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9265..10263
                     /gene="mglB"
                     /locus_tag="J1138_003389"
     CDS             9265..10263
                     /gene="mglB"
                     /locus_tag="J1138_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBA8455150.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10324..11844
                     /gene="mglA"
                     /locus_tag="J1138_003390"
     CDS             10324..11844
                     /gene="mglA"
                     /locus_tag="J1138_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBA8455151.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            11860..12870
                     /gene="mglC"
                     /locus_tag="J1138_003391"
     CDS             11860..12870
                     /gene="mglC"
                     /locus_tag="J1138_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBA8455152.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(13113..14348)
                     /gene="preA"
                     /locus_tag="J1138_003392"
     CDS             complement(13113..14348)
                     /gene="preA"
                     /locus_tag="J1138_003392"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBA8455153.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELHDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(14342..15580)
                     /locus_tag="J1138_003393"
     CDS             complement(14342..15580)
                     /locus_tag="J1138_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA8455154.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     IAADKIILAIGQHARLDAFAELEPQRNTIETQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGTCSC"
BASE COUNT         3766 a         3840 c         4115 g         3995 t
ORIGIN      
        1 acatcaccct ccggcagttg gaagtttttg cagaagtatt gaaaagtgga tcaaccaccc
       61 aggcatcggt gatgctggcg ttgtcgcaat cagcagtgag cgcagccttg accgacctgg
      121 aagggcagct tggcgttcaa ctgtttgatc gcgtggggaa aagactggtt gttaatgaac
      181 acgggcggct gctctatccg cgagcgttgg cactgcttga acaggcggtt gaaatcgaac
      241 aactgtttcg cgaagacaac ggcgcgattc gtatctatgc cagtagtacc atcggtaact
      301 acattctgcc tgcagttatc gcccgttatc gccatgatta tccgcagttg ccgattgaac
      361 ttagcgttgg gaatagccag gacgtgatgc aagcggtgct ggatttccgc gttgatattg
      421 gctttattga aggaccgtgc cacagcactg aaatcatttc tgaaccgtgg ctggaagacg
      481 agctggtggt tttcgccgcg ccgacttcgc cgttggctcg tggtccggtc accttagaac
      541 agctggccgc tgcgccgtgg atcctgcgtg aacgcggttc cggcacgcgg gagattgtcg
      601 attatctgtt gctgtcacat ttaccgaagt ttgagatggc gatggaatta ggtaactccg
      661 aggcaatcaa acatgcggtg cgtcatgggt tgggaattag ttgcctgtcg cgacgggtga
      721 ttgaagatca attgcaggca ggcacattaa gtgaagttgc ggtccctctg ccgcgcctga
      781 tgcgtacgtt gtggcgtata catcatcggc aaaaacacct ttccaacgcg ctacggcgct
      841 ttctggacta ttgcgatccc gcgaatgtac cgcgttaagg tgctgtacaa gaacatgctg
      901 gtgctgtgtc gatttcgtga cgcagcgccc tcagcatgca ttcgccagaa aagagattgg
      961 ctgctttact tataatccct gggcgatcat gaaggtgtct tataaccgtg tatttctgcc
     1021 ggaaggattg ccaatcgtct gctacaatcg cgcctcattt ttaagatgga tagcattttt
     1081 gtatgggttc cgaaactaaa actacagaag cgccgggctt acgccgtgaa ttaaaggcgc
     1141 gtcacctgac gatgattgcc attggcggtt ccatcggtac aggtcttttt gttgcctctg
     1201 gcgcaacgat ttctcaggca ggtccgggcg gggcattgct ctcgtatatg ctgattggcc
     1261 tgatggttta cttcctgatg accagtctcg gtgaactggc tgcatatatg ccggtttccg
     1321 gttcgtttgc cacttacggt cagaactatg ttgaagaagg ctttggcttc gcgctgggct
     1381 ggaactactg gtacaactgg gcggtgacta ttgccgttga cctggttgca gcacagctgg
     1441 tcatgagctg gtggttcccg gatacaccgg gctggatctg gagtgcgttg ttcctcggcg
     1501 ttatcttcct gttgaactac atctctgttc gcggctttgg tgaagcggaa tactggttct
     1561 cactgatcaa agtcacgaca gttattgtct ttatcatcgt tggcgtgctg atgattatcg
     1621 gtatcttcaa aggcgcgcag cctgcaggct ggagcaactg gacaatcggc gaagcgccgt
     1681 ttgctggtgg ttttgcggcg atgatcggcg tagctatgat tgtcggcttc tctttccagg
     1741 gaaccgagct gatcggtatt gctgcaggcg agtccgaaga tccggcgaaa aacattccac
     1801 gcgcggtacg tcaggtattc tggcgaatcc tgttgttcta tgtgttcgcg atcctgatta
     1861 tcagcctgat tattccgtat accgatccga gcctgctgcg taacgatgtt aaagacatca
     1921 gcgtcagtcc gttcaccctg gtgttccagc acgcgggtct gctctctgcg gcggcggtga
     1981 tgaacgcagt tattctgacg gcggtgctgt cagcaggtaa ctccggtatg tatgcgtcta
     2041 ctcgtatgct gtataccctg gcgtgtgacg gtaaagcgcc gcgcattttc gctaaactgt
     2101 cgcgtggtgg cgtgccgcgt aatgcgctgt atgcgacgac ggtgattgcc ggtttgtgct
     2161 tcctgacctc catgtttggc aaccagacgg tatacctgtg gctgctgaac acctccggga
     2221 tgacgggttt tatcgcttgg ctggggattg ccattagcca ctatcgtttc cgtcgcgggt
     2281 acgtattgca gggacacgac attaacgatc tgccgtaccg ttccggtttc ttcccactgg
     2341 ggccgatctt cgcattcatt ctgtgtctga ttatcacttt gggccagaac tacgaagcgt
     2401 tcctgaaaga taccattgac tggggcggcg tagcggcaac gtatattggt atcccgctgt
     2461 tcctgattat ttggttcggc tacaagctga ttaaaggaac tcacttcgta cgctacagcg
     2521 aaatgaagtt cccgcagaac gataagaaat aagtttcctc ccttccttgc taagccctct
     2581 caaccgagag ggctttttca attccatttc cctgacaaat catgcggata taaaatttaa
     2641 catttggatt gataattgtt atcgtttgca ttatcgttac gccgcaatca aaaaaggctg
     2701 acaaatcaga ggctgttccg gctttctggg atgatcacct gcatacaaat aagtccaccg
     2761 cgatgctgcc gtacgcaagg ggacgtaaag aagatgtgag cgatacccat tttattttcg
     2821 tagttacctc atggagatat ggaatgttta ggttgaaccc tttcgtacgg gtcgggctgt
     2881 gtttgtccgc tatttcttgt gcatggcctg tgttagcggt cgatgatgat ggcgaaacga
     2941 tggttgtcac tgcatcttcc gttgaacaaa accttaaaga tgctcccgcc agtatcagcg
     3001 tcattaccca ggaagacctg cagcgaaaac cggtacagaa tctgaaggat gtcctcaaag
     3061 aagtgcctgg cgtacaactg acgaacgaag gggataaccg taagggcgta agtattcgtg
     3121 gtctggacag cagctacacc ctgattcttg tcgacggtaa acgcgttaac tcccgcaatg
     3181 ccgtcttccg ccacaatgat ttcgatctga actggatccc ggtcgattcc atcgaacgta
     3241 tttaagtggt ccgtggcccg atgtcgtcgc tgtacggttc cgatgcgctc ggcggtgtag
     3301 tgaatatcat caccaaaaaa atcggtcaga aatggtcggg caccgttacc gtcgatacca
     3361 cccttcagga acatcgcgat cgcggtgata cctataacgg tcaattcttt accagcggac
     3421 cattaattga cggcgtgctg ggaatgaaag cttacggcag cctggcaaaa cgtgaaaagg
     3481 atgacccgca aaactcaacg accaccgata ccggagaaac gccgcgtatt gaaggattct
     3541 ccagccgcga cggcaatgtc gaatttgcct ggacaccgaa tcaaaatcac gattttactg
     3601 ccggatacgg tttcgaccgt caggatcgtg attccgactc gctggacaaa aaccgcctgg
     3661 aacgccagaa ctactccgtc agccataatg ggcgttggga ttacggcacc agcgaactga
     3721 aatactacgg tgagaaagtc gagaacaaaa accctggcaa cagcagcccg ataacttccg
     3781 aaagcaatac ggtcgacggc aaatacacgt tgccgctgac ggcgattaat cagtttctca
     3841 cggttggcgg tgaatggcgt cacgacaaac ttagcgatgc ggtgaacctg agcgggggaa
     3901 ccagctccaa aacgtctgcc agccagtacg cgctgtttgt ggaagatgaa tggcggatct
     3961 tcgagccgct ggcgctgacg accggcgtgc gtatggacga tcacgaaacc tacggtgaac
     4021 actggagtcc gcgtgcctac ctggtttata acgccaccga caccgtaacg gtgaaagggg
     4081 gctgggcgac ggcatttaaa gccccttctc tgttgcaact tagccctgac tggacgagca
     4141 attcctgccg tggcgcatgt aagattgtgg gtagcccgga tctgaaacca gaaaccagcg
     4201 aaagttggga gctggggctt tactacatgg gcgaagaagg ctggctggaa ggggttgaat
     4261 ccagcgttac cgttttccgt aacgatgtga aagatcgtat cagcattagc cgtacgtctg
     4321 acgtcaatgc tgcaccaggc taccaaaact ttgtcggttt tgagacgggc gctaacggac
     4381 ggcgcatacc ggtatttagc tactacaacg ttaacaaagc acgtattcag ggcgtggaaa
     4441 ccgaactgaa aattccgttc aacgatgaat ggaaactgtc gatcaactac acctacaacg
     4501 atggtcgtga tgtcagcaac ggcgaaaaca aaccgctatc cgatctgccg ttccatactg
     4561 ctaacggtac gctggactgg aaaccgctgg cgctggaaga ctggtcattc tatgtttctg
     4621 gtcactatac cgggcagaaa cgcgccgata gcgcgacggc taaaacaccg ggcggttata
     4681 ccatctggaa taccggcgcg gcctggcagg tgactaaaga cgtcaaactg cgcgcaggcg
     4741 tgctgaacct tggcgacaag gatctcagtc gtgacgacta cagctataac gaagacggac
     4801 gtcgttactt tatggcagtg gattatcgct tctgatgaga agatgcccgg cgaaccgggc
     4861 ggactttcac ttcagtaaat actgcgcatg gaagcgcagg tgatcctcta taaaagaggc
     4921 gatgaagtag taactgtgat catatcccgg ctgaatacgc agcgtcatcg gccacgcttt
     4981 ctggcgcgcg gcttctgcca gtactgcagg ttgcagttgg tcagcaagaa actgatcatt
     5041 atcaccctga tcaataagcg tcgggatcgc atcctgcgcg ttactggcat acatcagcgc
     5101 gcaactgtcc cattccagcc atgcattttt gtcctcacct aaataagtgc taaacgcttt
     5161 gattccccac gggacgctgc acggattcac aattggcgca aaggcggaaa cgctggtgta
     5221 tttacccgga tttttcagcg ccataatcag tgcaccgtga ccacccatgg aatgaccgct
     5281 aatggcgcag cggtcgctga cattaaattg cgactgaacc agcgccggta attcatcgcg
     5341 cagataatca tacatccggt aatgcgtcgc ccacggcggt tgcgtggcgt taagataaaa
     5401 ccccgcgccc tgtcctaaat cgtagccatc gtcgttggca acctgttcgc cgcgcgggct
     5461 ggtgtctggc atcaccagta caatccccag ttccgccgcc acgcgctggg cacccgcctt
     5521 ggtggtgaag ttctcgtcat tgcaggttaa tccggaaagc cagtacagca ctggtggcgg
     5581 ggtgtgatca cgaggtggtg ggagaaagat actgaacgtc atcgggcagt ttaaggtact
     5641 ggagtcgtgt cgccagcgtt gctgccagcc ttcaaaacag cggtgctctt cgagcatttc
     5701 catgcgtggc tccttgttgt gttgtttgca atttcttcat cataccgttt attcaatgtg
     5761 ctgtgagtaa ctttcacttc cgtatttgca taacgatgtt ttaacatctg ctgatgaaag
     5821 gcagcggcaa ttacaataat tatcgctgtg aatactggat tatgtgcgcc gcctcacgca
     5881 caataatcag gctgtaaatc agcttaataa ctttgccccc acgcagggcg gaggcgtcac
     5941 acctgcagga gaaatcataa atgccatcac tcagtaaaga agcggccctg gttcatgaag
     6001 cgttagttgc gcgaggactg gaaacaccgc tgcgcccgcc cgtgcatgaa atggataacg
     6061 aaacgcgcaa aagccttatt gctggtcata tgaccgaaat catgcagctg ctgaatctcg
     6121 acctggctga tgacagtttg atggaaacgc cgcatcgcat cgctaaaatg tatgtcgatg
     6181 aaattttctc cggtctggat tacgccaatt tcccgaaaat caccctcatt gaaaacaaaa
     6241 tgaaggtcga tgaaatggtc accgtgcgcg atatcactct gaccagcacc tgtgaacacc
     6301 attttgttac catcgatggc aaagcgacgg tggcctatat cccgaaagat tcggtcatcg
     6361 gtctgtcaaa aattaaccgt attgtgcagt tctttgccca gcgtccgcag gtacaagaac
     6421 gtctgacgca gcaaattctc attgcgctac aaacgctgct gggcaccaat aacgtggctg
     6481 tctctatcga cgcggtgcat tactgcgtga aggcgcgtgg catccgcgat gcaaccagtg
     6541 ccacgacaac gacctctctt ggtggattgt tcaaatccag tcagaatacg cgccacgagt
     6601 ttctgcgcgc tgtgcgtcat cacaactgat aaaaaggcag gaaccgtgga gcgcaacgtc
     6661 acgctcgatt ttgttcgcgg cgtcgcaatt ctggggatcc tgctattaaa catcagcgcc
     6721 tttgggttac caaaggcggc ttatctcaat cccgcctggt acggcgctat tacgccacag
     6781 gatgcatgga cctgggcatt tctcgatctc atcggccagg tgaaattcct cacgcttttt
     6841 gcgctgttgt ttggtgcggg cctgcaaatg ttgctgcccc gtggcagacg atggatccag
     6901 tcgcggttaa cgctgttagt cttgctgggc tttattcacg gtttattgtt ctgggacggc
     6961 gatattctgc tggcttacgg gctggtgggc ttaatctgct ggcggctggt gcgcgatgcg
     7021 ccatcggtaa aaagcctgtt taataccggc gtcatgcttt atctggtggg gcttggcgtt
     7081 ttgctgttat tgggggggat ttccgatagc cagaccagcc gcgcctggac acctgataca
     7141 tcggctattt tgtatgaaaa gcactggaaa cttcacggtg gtgttgaagc gatcagtaat
     7201 cgtgccgatg gtgttggcaa cagtttactg gcactgggcg cacagtatgg ctggcaactg
     7261 gcagggatga tgctcattgg tgccgcattg atgcgcagtg gctggctgaa agggcagttc
     7321 agcttacgtc actatcgtcg tactggtttt gtgctggtgg cgattggggt gatcattaac
     7381 cttcctgcca tcgccctgca atggcgactg gactgggcgt atcgttggtg cgccttctta
     7441 cttcagatgc cgcgggaact gagtgcgccg tttcaggcga ttggctatgc gtcgctgttt
     7501 tatggcttct ggccgcaatt aagccgcttt aagctggtgc tggcaatcgc ctgtgtcgga
     7561 cggatggcac tgaccaacta tctattgcaa acgctgattt gtactacgct tttttaccac
     7621 ctcggtttgt ttatgcagtt tgaccgtctg gagctgctgg cgtttgttat tccggtatgg
     7681 ttggcgaata tcttcttctc tgttatctgg ctgcgttact tccgccaggg gccggtggaa
     7741 tggctctggc gtcagttaac tttgcgtgct gccggaccga caatatctaa aacatcaaga
     7801 taacgatctg gatcacattc gttaacaaaa cggctgtaac cgtttccatt gctgtgactc
     7861 gattcacgaa gtcctgtatt cagtgctgac aaaatagccg ccagcaagca gtcatttact
     7921 gcaatctcat aacaggtagt gaatatgatc accattcgtg atgtagcgcg tcaggctggc
     7981 gtctctgtgg caacggtttc ccgggtgctc aataacagca cgctggtcag tgccgatacg
     8041 cgtgaagcag taatgaaagc cgtgagtgag ctggattatc ggccaaacgc caatgcccag
     8101 gcgctggcaa ctcaggttag cgataccatt ggcgtggtgg tgatggacgt ttctgatgcg
     8161 tttttcggcg cgctggtaaa agcggtggat ctggtcgccc agcagcatca gaaatacgtg
     8221 ctaatcggca atagctatca tgaagcggaa aaagagcgtc acgccattga ggtgttaatt
     8281 cgccagcgtt gtaatgcgtt gattgttcac tcaaaagcat tgagtgacga tgaactggca
     8341 caatttatgg ataacattcc cggtatggtg ttaatcaacc gcgttgtgcc ggggtacgcc
     8401 catcgttgcg tttgcctgga taatctcagc ggtgcccgaa tggcgacgcg catgttgctg
     8461 aataacggtc atcaacgtat tggttatctt tcttccagcc acggcattga agatgacgcc
     8521 atgcgtaaag caggctggat gagtgcgttg aaagagcagg atattattcc gccggaaagc
     8581 tggattggca ctggcacgcc ggacatgccg ggcggtgagg cggcgatggt tgaactgctg
     8641 gggcgcaatc tacaactaac cgctgtattt gcttataacg acaatatggc cgctggcgca
     8701 ctgacagcat taaaagataa tggcattgcg attccgttac atctctcaat cattggtttc
     8761 gatgatattc ccatcgcccg ttacaccgac ccgcaattaa cgactgtgcg ttatcccatt
     8821 gcttcaatgg ctaaattagc taccgaactg gccttgcagg gggcagcaga caatattgat
     8881 cctcgtgcca gccactgttt tatgccgacg ttagtgcgtc gccattctgt cgcaacacgc
     8941 cagaatgcgg cggcgatcac taactcaaca aatcaggcga tgtaaccgct ttcaatctgt
     9001 gagtgatttc acagtatctt aacaatgtga tggctatgat tgcaccgttt taacgttgta
     9061 acccgtatgt aacagtgaat aatcactttc accgaggtaa tagcgtcata acaacaatta
     9121 aagccgtttt ctggagcgtt accgggcatg gaagaacgaa ttttaaaaag tgagcttcgg
     9181 cgttcagtaa cacttcatta actctactgc cccgccgagc atttatctca agcactaccc
     9241 tgcataagaa aaaccggaga taccatgaat aagaaggtgt taaccctgtc tgctgtgatg
     9301 gccagcatgt tattcggtgc cgctgcacac gctgctgata ctcgcattgg tgtaacaatc
     9361 tataagtacg acgataactt tatgtctgta gtgcgcaagg ctattgagca agatgcgaaa
     9421 gccgcgccag atgttcagct gctgatgaat gattctcaga atgaccagtc caagcagaac
     9481 gatcagatcg acgtattgct ggcgaaaggg gtgaaggcac tggcaatcaa cctggttgac
     9541 ccggcagctg cgggtacggt gattgagaaa gcgcgtgggc aaaacgtgcc ggtggttttc
     9601 ttcaacaaag aaccgtctcg taaggcgctg gatagctacg acaaagccta ctacgttggc
     9661 actgactcca aagagtccgg cattattcag ggtgatttga ttgctaaaca ctgggcggcg
     9721 aatcagggtt gggatctgaa caaagacggt cagattcaat tcgtactgct gaaaggtgag
     9781 ccggggcatc cggatgcaga agcacgtacc acttacgtga ttaaagaatt gaacgacaaa
     9841 ggcattaaaa ctgaacagtt acagttagat accgctatgt gggataccgc tcaggcgaaa
     9901 gataagatgg acgcctggct gtctggcccg aacgccaaca aaatcgaggt ggttatcgcc
     9961 aacaacgacg cgatggcaat gggcgcggta gaagcactga aagcacacaa caagtccagc
    10021 attccggtgt ttggcgtcga tgcgctgcca gaagcgctgg cgctggtgaa atccggtgca
    10081 ctggcgggca ccgtactgaa cgatgctaac aaccaggcga aagcgacctt tgatctggcg
    10141 aaaaacctgg ccgatggcaa aggtgcggct gatggcacca actggaaaat cgacaacaaa
    10201 gtagtccgcg taccttatgt tggcgtagat aaagacaacc tggctgaatt cagcaagaaa
    10261 taagactgat tgggcgcagt cgtttactgc gcccttattg tatgaccaac aaggtaaaat
    10321 tatatggtca gctcaacgac tccgtcttcc ggggaatact tgttggaaat gagcggtatc
    10381 aacaagtctt ttcctggtgt taaggcactt gataacgtta acttaaaagt ccggccacat
    10441 tctatccatg cattaatggg ggaaaatggt gcgggaaaat caacattatt aaaatgcctg
    10501 tttggtattt atcaaaaaga ctccggcacc attttattcc agggtaaaga gatcgatttc
    10561 cattccgcca aagaagcact ggaaaatggt atttcgatgg tacatcagga gttaaacctg
    10621 gtattacaac gttcggtgat ggacaacatg tggctggggc gatatcccac caaaggcatg
    10681 tttgtcgatc aggacaaaat gtaccgcgaa accaaagcga tttttgatga actggatatt
    10741 gatatcgatc cgcgtgcgcg cgtcggtaca ttatctgttt cgcaaatgca gatgatcgaa
    10801 atcgccaaag cgttttccta taacgcgaaa attgtgatta tggatgaacc gacttcttcg
    10861 ttaaccgaaa aagaggtcaa tcatctgttc actattattc gtaaattaaa agagcgcggc
    10921 tgcggtattg tttatatctc gcataaaatg gaagaaatct tccagttatg tgatgaagtt
    10981 accgtattgc gcgacggtca gtggatcgct accgaaccgc tggcaggact gacgatggac
    11041 aagatcatcg ccatgatggt tgggcgttct cttaaccagc gtttccctga caaagaaaac
    11101 aagccgggcg aagtgattct ggaagtgcgt aacctgacgt cgctgcgcca gccgtcgata
    11161 cgcgatgtct cgtttgatct gcataaaggg gagattctcg gtatagccgg tctggtggga
    11221 gcgaaacgta ccgatattgt tgagacgtta tttggtattc gcgagaaatc ggctggcacc
    11281 attacgttgc acggcaaaaa gatcaataac cataatgcca acgaagccat aaaccacgga
    11341 tttgcactgg taactgaaga gcgccgctca acgggaattt atgcctatct ggatattggc
    11401 tttaactcgt taatttccaa tatccgcaac tataaaaata aagttggttt actggataac
    11461 tcgcggatga aaagcgatac ccagtgggtg attgattcga tgcgggtaaa aacaccgggg
    11521 catcggacgc aaattggttc gctctccggt ggtaatcagc aaaaagtgat tattggtcgc
    11581 tggctgttaa cgcaaccaga aatattaatg ctcgatgaac cgacgcgcgg tattgatgtt
    11641 ggggctaagt ttgagattta tcagttaatt gccgaactgg cgaagaaagg caaggggatt
    11701 attattatct cctccgaaat gccagagttg ttagggataa cagaccgtat tctggttatg
    11761 agcaatggtc tcgtttccgg aattgtcgat acaaaaacaa caacgcaaag cgaaattctg
    11821 cgtcttgcgt ctttgcacct ttaagattag gggcttccca tgagtgcgtt aaataagaaa
    11881 agttttctta cttacctgaa agagggcggt atttacgtcg ttcttttagt tttgctggcg
    11941 attattattt tccaggaccc aacattttta agtctgttga acttaagtaa tattctcacc
    12001 cagtcatcgg tgcgtattat tatcgcgctt ggtgtggcag ggttaattgt cacccagggg
    12061 actgaccttt ccgcaggtcg ccaggtgggg ctggcggcag tagtagcagc gacgttattg
    12121 cagtccatgg ataacgctaa caaagtgttc ccggaaatgg cgacgatgcc gattgcgctg
    12181 gttattctga ttgtctgtgc cattggtgcg gtgatcggtt tgatcaacgg gctgattatc
    12241 gcctatctca acgtgacgcc gttcattacc acgctcggca cgatgattat cgtctacggc
    12301 attaactcgc tctattacga ctttgtcggg gcgtcgccaa tttccggttt tgacagtggc
    12361 ttctctacct ttgctcaagg ctttgtcgcg ctggggagtt tccgtctctc ttacatcacc
    12421 ttctatgcgt tgattgcggt ggtgtttgtc tgggtgttat ggaacaaaac ccgcttcggt
    12481 aagaacattt ttgccattgg cggtaacccg gaagcggcga aagtatctgg tgtcaacgtc
    12541 ggcctgaacc tgttgatgat ctacgcgttg tctggtgtgt tctatgcctt cggcgggatg
    12601 ttagaagccg gacgtatcgg ctcagccacc aacaacctcg gctttatgta tgagctggat
    12661 gctatcgcgg cgtgcgtggt aggcggcgta tcgttcagcg gcggtgtggg gacggtaatt
    12721 ggtgtggtga ccggggtaat tatttttacc gtcattaact atggcctgac gtatatcggc
    12781 gtaaacccgt actggcagta catcatcaaa ggggcgatta ttatcttcgc cgtggcgctg
    12841 gattcactga aatacgcgcg taagaagtga tgtgattgtg gtccgcattc gttcataaac
    12901 gcacataaca tcatgatctg tgtggttttg tagtccggat aaggcgttca cgccgcatcc
    12961 ggcgacagga gccaaatgcc ggatgcaacg ctggcgcgtt ttatccggcc tacaatgagc
    13021 acaacatact gatttatatt gttttgtatg ccgggtaagg cgttcacgcc gcatccggcg
    13081 acaggaacta aatgccggat gcgtcgctgg ttttacaacg ttaccgggtg ttctttctcg
    13141 cctttcttaa acttcacttc cccaagatca atacaaccta ccgggcagac atgaccacaa
    13201 agcagacaac ccacgcattt ctcggtatta caatgcggtg tgcgggtttt ctcgctccat
    13261 tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac agcgtccaca gccaacacat
    13321 ttatcaagat tgatatgggg atagacaata taactgcggt ctaaatcttc cgccgggaca
    13381 atattgtgat tcgccagacc caccatctcc tgcaacgaat caaacccttg atcggcgaga
    13441 taatgcgaca acccgcttgc catatcttcc actatccgat acccgtactg cataatgccg
    13501 gtggtcacct gcaacgttgc tgcgccgagc aataaaaact ccgccgcatc ttcccaggtt
    13561 tcaatgccgc cgataccgct gatcgggaaa tcgtgcagtt ctggatgggt gcgcatttgc
    13621 tgaataaagc gtagggcgat cggttttacc gctttaccgg aatatccgga aatactcgat
    13681 ttaccgttaa cgatcggcat accaattttc tgattaagat cgatattggt gatggattta
    13741 acggtgttaa tcgccgcaat gccatctgcg ccgccgcgct tcgccgccag cgccacttcg
    13801 cacatatcac caatattcgg cgtcatcttc gccagcattg gcagcgtgga accacgtttt
    13861 actgcccgac aatatttttc taccagctcc gggctttgcc cgacatcgct acccatcgca
    13921 tgagaagtca tttgcggaca ggagaagtta cactcgatca tatccgcgcc agcttcttgc
    13981 accagacgcg ccagctcttc ccattgctgc tcattttcgc ccatgattga agcgatcaat
    14041 actttgtccg ggtaatcttc tttcagccga cgcagggcgg ccagattctc ttccaacgga
    14101 tgttcagcaa tctgctccat atttttgaag ccgataaaac cggtatcttc tttcaccaga
    14161 tgatcaaaac gcggcgagac ttcgttggcg ataaaaaaac cgatcgtttt aaacaccacg
    14221 ccgccccaac ctgtgtcgta ggctttggca cacatctcat agcagttgcc taccggcgaa
    14281 gaagaaaggc agaacgggtt gggaaacttc acgccgcaaa aagtaatcga aagatctttc
    14341 gttaacatga gcaagttccc tctaaatagt gatgaatcgc ctcggcggct tctttcccgg
    14401 ttttcactgc atagaccacg gttttgtcac cctcaacaat atcgccagca gcaaagactt
    14461 gcgggtcgcg ggtctggtaa ttttgcgttt cgatggtgtt acgctgcggc tctaactccg
    14521 caaaggcatc cagtctggca tgctgaccga tggcgagaat aattttgtcc gccgctatcg
    14581 ttagttcacc cggtaaacga acatgcttaa aggtgacttt attgccttcc acggctactg
    14641 gtgtaaatcc atcgatgatc gaaacgccca gctctctggc gctggtaaac tctttttcgc
    14701 ttgccggaaa ctcatctaac tcttcacgcg ctacgcaggt taccgcctga cagccgagaa
    14761 ctttcagcgt gctggctacg tccatcgcga catcaccgcc gccgataatt aatgcgcttt
    14821 gtggaatgct gatatcgcct tgcgcctgtc gtgcacgttg caggaagtcg acggcaatct
    14881 caacgtcgct atgctcaaac agcgataacc cggaaccgct cgataaccct acggtgacca
    14941 gtaccgcgcg gttttccgct ttcagctgct ccagggtaag tgttttaccg atttcgttat
    15001 tgcacttaat ggtcacgccc attttttcta tacgggcgat ttctgcatcc agcaccgatt
    15061 gcggtaaccg gaattgcgga atgccgttac gcaaccagcc accgggttgt gcttctttct
    15121 catagatcgt gacgtcataa ccctggtttg tcagcgtcac actggcctgc aatccggcag
    15181 gacctgcgcc aataatggcg actttgccga gcgttttagt accgggctga taaatttcca
    15241 tcccagtttg ttgttcaaaa tcagtaacaa aacgctgtaa acgaccgata tcaatgggag
    15301 catcaacacc ggcacgtgta caaccgcttt ggcataattt ctccgtcggg cacactctgg
    15361 cacaaacggc accgagggca ttattttcgc gaattgtctc ggcagcgcct ttaaaattac
    15421 gaaagtagat tgagcgaata aatttccccg gatcggtctg tgccgggcaa gcctgactac
    15481 agggagcgtc atgacataat aaacagcgag aggcttcttt aatcgccagt aaaggcgtaa
    15541 aagccggagt gagttcatcc agataatttt gttgcggcat gtgatcttcc ttaatgctat
    15601 gagtaattaa ggcgaacgct aaataaatat tgcgtgctga tattaaggct atgcgcgtgg
    15661 ggaaatttaa actgtgaaag gattcacaaa tagctgcggt tcagaatgtg atatcc
//
LOCUS       DADTFG010000116        15557 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.117, whole genome shotgun sequence.
ACCESSION   DADTFG010000116 DADTFG010000000
VERSION     DADTFG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.117"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            36..602
                     /locus_tag="J1138_003394"
     CDS             36..602
                     /locus_tag="J1138_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBA8455155.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLSDAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            606..1181
                     /locus_tag="J1138_003395"
     CDS             606..1181
                     /locus_tag="J1138_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBA8455156.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGNLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1223..2275)
                     /locus_tag="J1138_003396"
     CDS             complement(1223..2275)
                     /locus_tag="J1138_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBA8455157.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPEPTE"
     gene            2500..3420
                     /locus_tag="J1138_003397"
     CDS             2500..3420
                     /locus_tag="J1138_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HBA8455158.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3592..4818
                     /gene="mdtG"
                     /locus_tag="J1138_003398"
     CDS             3592..4818
                     /gene="mdtG"
                     /locus_tag="J1138_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HBA8455159.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKIMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKIMITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            4901..5275
                     /locus_tag="J1138_003399"
     CDS             4901..5275
                     /locus_tag="J1138_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HBA8455160.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(5276..5503)
                     /locus_tag="J1138_003400"
     CDS             complement(5276..5503)
                     /locus_tag="J1138_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBA8455161.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDVHHGPYE"
     gene            complement(5675..8218)
                     /gene="mdoH"
                     /locus_tag="J1138_003401"
     CDS             complement(5675..8218)
                     /gene="mdoH"
                     /locus_tag="J1138_003401"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBA8455162.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8211..9746)
                     /gene="mdoG"
                     /locus_tag="J1138_003402"
     CDS             complement(8211..9746)
                     /gene="mdoG"
                     /locus_tag="J1138_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBA8455163.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10140..11297
                     /gene="mdoC"
                     /locus_tag="J1138_003403"
     CDS             10140..11297
                     /gene="mdoC"
                     /locus_tag="J1138_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBA8455164.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11305..12786)
                     /locus_tag="J1138_003404"
     CDS             complement(11305..12786)
                     /locus_tag="J1138_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBA8455165.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPQLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDNILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHDDAMTHRYLRKMESSPFINHLVDGTLSLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHSKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(12728..13261)
                     /gene="ymdB"
                     /locus_tag="J1138_003405"
     CDS             complement(12728..13261)
                     /gene="ymdB"
                     /locus_tag="J1138_003405"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBA8455166.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13356..13667)
                     /locus_tag="J1138_003406"
     CDS             complement(13356..13667)
                     /locus_tag="J1138_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA8455167.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFNGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13788..14120)
                     /gene="csgC"
                     /locus_tag="J1138_003407"
     CDS             complement(13788..14120)
                     /gene="csgC"
                     /locus_tag="J1138_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBA8455168.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQLIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPSSSEKS"
     gene            complement(14179..14637)
                     /gene="csgA"
                     /locus_tag="J1138_003408"
     CDS             complement(14179..14637)
                     /gene="csgA"
                     /locus_tag="J1138_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBA8455169.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14678..15133)
                     /gene="csgB"
                     /locus_tag="J1138_003409"
     CDS             complement(14678..15133)
                     /gene="csgB"
                     /locus_tag="J1138_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBA8455170.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3882 a         3979 c         3671 g         4025 t
ORIGIN      
        1 tttctttctc tcgcaaattc gttttggatg tacttatgtc attcacaaat acccctgaac
       61 gctatggtgt tatatctgcg gccttacatt ggctaagcgc aattattgtc tatggcatgt
      121 ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat caccaggcac
      181 cggaactgca taaaagcatc ggcattttgt taatgatggg gctggttatt cgcgttctgt
      241 ggcgcgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg
      301 cagcaaaagc tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct
      361 atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg
      421 cgaccctctc cgacgctggc gcacaggctg actttgccgg tgccctgcat ttttggctcg
      481 cgtggagcgt tgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca
      541 tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat
      601 aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcttc cttgatgttc tctgccggtt
      661 cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaacttcc
      721 gcatccagca tcttggctat agctggttat atggcacctt caaagatttc gacggtactt
      781 ttacctttga tgaaaaaaat ccagctgccg ataaagtgaa tgtgacaatt aacaccacca
      841 gcgtcgatac taatcacgcc gaacgcgata aacatcttcg cagtgcggat ttcctcaata
      901 ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac
      961 tggatattac cggcaatctg acgctgaatg gtgtaaccaa gcccgtcaca ctggaagcga
     1021 aattaattgg tcagggtgac gacccgtggg gcggtaaacg tgcaggcttt gaggccgaag
     1081 gcaaaatcaa gctcaaagac ttcaatatca agacagattt aggtccagcg tctcaggaag
     1141 tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat gttgccggat
     1201 ggcacgcatc cggcaatgat atttattctg tcggttcagg aatgcccagt gttgtattca
     1261 gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc
     1321 gctgttcctc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt
     1381 atttttccgc acatactgga cactgaataa acaacagatg gcagccatca tttttacagt
     1441 tggtatggct gtcgcacggc gcaccgcact ggtggcagtg cgcgataatc tcatcagata
     1501 tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc
     1561 cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt
     1621 tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt
     1681 acatgacgat ttttttatct ttatgtgcct gcatcatctc aactgctttt ggcagctgct
     1741 cacggaaggt atctgccggt atttccagcg cgttttcaaa gtgccccact tcatactcat
     1801 agtggttacg catgtcgata aacaatgcat cgggatcgtc aagcatggcg ttcacttccg
     1861 ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg
     1921 caacaatgcg atcgcgtacc ttcatacgca gtacccagaa ggatttcccg tcatcatcca
     1981 acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa
     2041 atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat
     2101 acactcgccc aaaaacattc agcgcggtaa acagttgata taaagcgtca cgggtcacct
     2161 taggatcggc aatgtggaaa tacttataaa acgaaatggt ggttcgcggt tcgctctcag
     2221 ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt
     2281 acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat
     2341 taacatccac acattttacg ctacatttta gcattaaaaa ttatttgtta tttacaagcg
     2401 cggcaatttc gcccagtctt cagtcacaaa ttttggttgc ggacgaaaaa atgcgacaat
     2461 acatacaatt gcccgtatag gttgaaaaac aggattgata tgacgaatct acccaagttc
     2521 tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg
     2581 ttggtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg
     2641 gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc
     2701 ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc
     2761 atgggcctga tggaaaccgg catggcgtgg ttctggccgg accgccgaat cgcccgctgg
     2821 acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg
     2881 ttagttggca tccattttct gacactggag ctgggtgcgc ggcagtttgg tatgcaggaa
     2941 ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg
     3001 ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa
     3061 gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca
     3121 agcgttttcg tcccgttgtt tgccgttgag caggctgcga ccacgaccgg aacctggatg
     3181 ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc
     3241 aaagggtatc agttgattat actgccgcca gagtgttctc cgccgctgga tgatgccgaa
     3301 actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag
     3361 tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa
     3421 atcttccatg ctggatgctt cataatggca tccggcatta ccacagaaaa tccccctgat
     3481 ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct
     3541 tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc
     3601 tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt
     3661 tttcttaccg gtgccgcctt cagcctggta atgcccttct tacccctcta cgttgagcag
     3721 cttggcgtta ccggccactc cgccctgaat atgtggtccg gtattgtctt cagcattaca
     3781 tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg taaaggccga
     3841 aaaatcatgc tattacgctc tgctctcggc atgggcatcg tgatggtgtt gatgggactg
     3901 gcacaaaata tctggcagtt tttgatcctg cgggcgcttc ttggtttact tggcggattt
     3961 gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag cggctgggcg
     4021 ctgggtacgc tctccacagg cggcgttagc ggcgcgttgc tcggcccaat ggctggcggt
     4081 ttgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag tgtgctcata
     4141 ctctgctttt tcgtcaccct gttttgcatc agagaaaaat tccagccggt cagcaaaaaa
     4201 gagatgctgc atatgcggga agtggtgaca tcacttaaaa acccgaaact ggtactcagc
     4261 ctgtttgtca ccacgttaat catccaggtg gcgacgggct caattgcccc cattctgacg
     4321 ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag tggcatgatc
     4381 gcctcggtgc caggcgtggc ggctctgcta agtgcaccac gactcggcaa acttggcgat
     4441 cgaatcggac ccgaaaagat catgattacg gcgctgatct tttctgtact gctgttgatc
     4501 ccaatgtctt acgttcagac gccattacaa cttgggattt tacgtttttt gctcggtgcc
     4561 gccgatggtg cactactccc cgccgtacag acactattag tttataactc gagcaaccag
     4621 atcgccgggc gtatcttcag ctataaccaa tcgtttcgtg atattggcaa cgttaccgga
     4681 ccattgatgg gagcagcgat ttcagcgaac tacggtttca gggcggtatt tctcgtcacc
     4741 gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg tcgtcgaatc
     4801 ccccagatat cgaactgatt tttcgccttt catacttgca aaagcggaga atcagctatc
     4861 cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt acgcaacgct
     4921 tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg gcatcgacgc
     4981 cgaaaacgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg acggcgacat
     5041 catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt tacccatgct
     5101 tagcggtgaa gctgcgcaaa gtgtctttga cggcgactat gatgagatag agatccgcca
     5161 ggagtggcaa gatgagaata cattacatga atgggacgag ggggaatttc agcttgagcc
     5221 accgctggat accgaggaag gacgcactgc agctgatgag tgggatgaac gttaatcact
     5281 catacgggcc atgatgtacg tcgatcggca gcagtaaagt atcgaaaacc agggagaatg
     5341 gcagatcaag gatcgtgacg taacgccagg cggagtcacg cacgtcccat tgcacaccgg
     5401 gataatattg gttgccatgc ccctgccccg gtatagtgcg actaatgatg ctgccacagc
     5461 cactcagcaa ggtcaccatt atgctcatca caattaaacg catttgatac ctcttccgta
     5521 aaaaataccg gcataacgcc ggtatttttg ttggctcgtt gtttttgccg gatgcggcgt
     5581 gaacgcttta cccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg
     5641 cctgataagc gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac
     5701 gtaatgccag tggattgagc tttatccctt cgtaataact cacccatgag gaatatctct
     5761 ccggggaatt ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca
     5821 ccaggcgacg atcgcgattc agcttctctg gcgtctcgtt cagcgcctgt tcaacgtggc
     5881 ggtcacgggc gatttccagc accttgctgg cgcggtgacg cgcggtcgcc attgcggttg
     5941 ccagagcgtt aaatgacggg ttaaacaccg cgtgcataaa accatcatca agggagcgtt
     6001 gacgattcat ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt
     6061 ccgggatcag gaacagtttc cagcgtttgg ttcgcagacc aacggtggca cggctggaaa
     6121 tcaccgaaac aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagaa
     6181 aacgcagatc caaccacgcc ataccaactg cccacactaa ccctaacagc agctgtgagc
     6241 cgtggcgttt gaacgcttcc ccccaggaag tggagtcatc atcacgctgc ggtgaattcc
     6301 acaccacttc ccagccaagg aacgcgctga cgacgaagac cgtatggaac agcatgcgta
     6361 ccggagccag cagcacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga
     6421 agccgccgta ttctttcgtt cctttgcacc agataagcaa aatgctcaat agcttcggca
     6481 ggaacaacag caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg
     6541 gccataccgg gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgta
     6601 ctacctgcaa tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag
     6661 acatcacgcc cgtcaggaac accgcacggt gaaccgggtg catacccttc accaggaaca
     6721 gacggaagtt catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca
     6781 agttaggcgg caattcttca taagaacccg ggagatcgta agcaatccag accccccaac
     6841 ctgcacggcg catcaacgcc gcttccacga agtcatgtga caggattgaa ccggcaaaag
     6901 aaccttcgcc cggcagcgga gccagtgcac agtgctcgat aaacggtttc acgcggataa
     6961 tcgcgttatg cccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa
     7021 acagtggccc atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatgc
     7081 cggacgcttt cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaggcgca
     7141 ccaggccgca caaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc
     7201 tgtactggct gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc
     7261 gacggcggcg gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca
     7321 tccaggcttt ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga
     7381 catcaaaatg tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag
     7441 caaaaacacg gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg
     7501 ggtttaatgg ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa
     7561 gcagttgcag gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta
     7621 ccgcaaagag gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa
     7681 cccacacatc ctgaccaacc atatccatag gattaatcag cgcccacccc tgataaggaa
     7741 gaatggtctt catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa
     7801 tgtaacggcg gatggtaccg acagtacgcc acttttgctc gctctcctgc tcttctttgg
     7861 tcaaacgagc cagatagcgc ggcgtcacat cgcgtccacg caggcgatcc cagaaacggc
     7921 ctaccgggtt ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg
     7981 cctttagctg atcgcgccct tcgtcgtctt tgattaactg tccatcagca agtgaatctg
     8041 gccaggcttg ttccagacgc gcctttaccg agccttgcgg ggagtcatcc tcccgcgccc
     8101 aggtgcggtg ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg
     8161 ccgctttctc gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg
     8221 gcaggtaact ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca
     8281 cgcatttcag tggttttctt ggcatctttc actttcacac gcatcaccag acgccaacct
     8341 ttggtaaccg ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg
     8401 gtttgcgctg tgaccggggt atcctctggc agttttttca tctcagcgcc ggtaaaatcg
     8461 accacaaagg cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc
     8521 gttgaacgac gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca
     8581 cggctgaagg tgatggtgta tttaaagttc atctctttac ccggctccgg caactgatcc
     8641 ggcgtccagt aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc
     8701 tcaacgctgc ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acgaagatcg
     8761 taacgatcat cgagatcttc aaagcgggag aaatcacgac cacgctgcaa tagaccgaag
     8821 ccttgcgggt tttccatcga gaagctgctg accgctaaat gtttcgggtt attcaacgga
     8881 cgccagatcc attcgccatt accagcatgg atagacagac cgttagagtc gtgcaactcc
     8941 ggacgatagt tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac
     9001 ggtgcaaccc ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca
     9061 accgtgtcac gtcctggcat aactacgaat ttgtaagcac ctgtcgcgcg cggcgagtca
     9121 agcaatgcat aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct
     9181 ttgaagcgtg gaaattcttc acccgatggc aaggcggtat caattgccag gccgcgtgca
     9241 gaaaggccat aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg
     9301 acgatttcat cgtttttatc tttgctgttg atcgggtaaa gcactttgaa accggcaaaa
     9361 ccaaggtctt ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg
     9421 ctgtatttga ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg
     9481 aagtacatac cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac
     9541 gctttgtcat gattaaactg gatctgctga tagtccgcgt atttcatatc gcggaaaacg
     9601 gagggcaagt tgcttttggg cgcctcatag cctttcccgg ctaaggattg agcttgcttt
     9661 gcgacatcat caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca
     9721 gcactcaacc aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc
     9781 cccctttgtg tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa
     9841 cagaatcccg gtatcgttca gcacactggc tcatggatta agcacctgat tgcaaccgtt
     9901 ttcactttcc gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca
     9961 aatttattta tcaaccgtct tactgacgct tccattctgg ttagtggtag cgatgatgct
    10021 atgcataaaa accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca
    10081 agcctaaagg cataacccga cagggataag acgaaaaatc cgagattagt taaccatata
    10141 tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt
    10201 tgttagggat cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata
    10261 gcgccgaacc gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc
    10321 aggtgttttt cgttatatcc gggtactttt cctacatgct ttttttacgc tatcccttga
    10381 aaaaatggtg gaaagtacgt gtcgaacgtg tagggatccc gatgttaaca gccatcccct
    10441 tactgacatt gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc
    10501 ctgggctgtc attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt
    10561 ggtttttact ggtgttagtg gtcatgacga cgctgtgcgt atggatattt aaacgcatca
    10621 gaaataattt agaaaattct gataaaacga ataaaaaatt ctcgatggta aaattatcgg
    10681 tgattttttt gtgcctcggc atcggttatg cggtaataag aagaacgatt tttattgtgt
    10741 atccacccat tctgagtaac ggcatgttca attttattgt catgcaaacg ttattttatt
    10801 tgccgttctt tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta
    10861 ccacgccgtc tcgtggctgt acccttgcag cagcattggc gtttgtcgct tatttactca
    10921 accagcgcta tggcagtggc gatgcctgga tgtatgaaac cgagtcggtg atcactatgg
    10981 tcctcggtct gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc
    11041 agtcagcgcg ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc
    11101 cgttaacgct gtttttcggc gcatacatta caccacacat cacctccaac tggcttggtt
    11161 ttctctgtgg cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac
    11221 gcatcccatt attgaagttt ttgttctctg gtaaaccggt tgttaagcgt gagaacgata
    11281 aagcaccagc ccgttaagcc gcgtttacaa taaccattcc acgggcaata tcgacgccag
    11341 tttgaccata acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt
    11401 ctttgaatga cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc
    11461 cgcatcatac tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc
    11521 tatcacgaag cccatttcag tattgagcaa cgttgaacgc ggatcgaaat tgaaagagcc
    11581 gataaacacc gttttaccat cgatactaaa ggttttagca tgcaggctgg cccctgaatt
    11641 accggttatg ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata
    11701 taattccacc ccatagcgga gcaatttttt gcgccagcgc gcgtatcctg catggacgac
    11761 agcaacatcg ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac
    11821 catccgtaag agttgcgcca cacctgcacg tgtcggtaca aaataggaag agataatatc
    11881 gatgcgttca ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg
    11941 ttttgccttg ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat
    12001 aagcgacaac gttccatcaa ccagatgatt tataaacgga ctggattcca ttttgcgtaa
    12061 ataacgatgc gtcatggcat cgtcatgcca ggatgccggt aactcgatgc gatccgccat
    12121 ttcaccttcc gggacatcca gcacctgctg taagggggaa accgatttgc aataccaata
    12181 gcgggcgaaa tcatcggcaa cgtcctctac cacgggtcct attgccatga catctaaatc
    12241 cgaaaaaagt ggctcttccc ctgcaccaaa ataggcatca ccaatatttc gtcctcccac
    12301 cagcgtcacc acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga
    12361 aaagtcggtg atataaccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg
    12421 gacttcaatg cgtggatggc tgtcaagcaa gcgtaaaatg ttatcaagtc cgggcgtatt
    12481 gttgtcgtcc agcaacaaac gtacgcgaac gccacgcttt gctgcggcta acagggcgga
    12541 aaacagtaac cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagtgtatg
    12601 ctctgccatt tcagccagac gataccgggc ggcaaacgca tccagacttt tctccagagg
    12661 aaaaagtcca cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagctg
    12721 aggcaaatca ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt
    12781 catcataaca gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa
    12841 cggtttttac tgcgatttca gccgctgccg cacgggggta accataaacc ccagtactga
    12901 ttgcaggaaa agccactgac gtatagctgt ttgccgccac cagtcgtagg ctattgagat
    12961 aggcatcctg caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg
    13021 tgtgcactac ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag
    13081 ggcaatcgcc ctgctgctgc ctgactttta aacaggcatc cagcagggcc ggacccgctg
    13141 cgcgatgaat ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca
    13201 caatcacatc aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca
    13261 taacgcctcc ttacaccttc ttgtttccta agtgtacagc aggctttttg gttaaatcgc
    13321 atttttctgc tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca
    13381 gccagctttt tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta
    13441 acattgccgc tcattaacgc tttgctggaa taggttttac gcggaacaga accattctgt
    13501 gggcagttca tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt
    13561 tggccattaa aatggatcac gcccccttgc gcggtgcttc ctgcaatggc aataaaagga
    13621 aaaaacaaac tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc
    13681 tcaatgatta gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca
    13741 gcagaccatt ctctccaggt tcatcttatg ctcgatattt caacaaatta agacttttct
    13801 gaagaggacg gccattgttg tgataaatga agtgactgtc catcagaaac agtaacaact
    13861 attttcaccc gatcgtccgg ggaaatattt aaactcaact tcgtcaaagc aatgagttga
    13921 ttagcaggca atgaaagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg
    13981 cgcagggaca atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata
    14041 atggtgtaca tatccccttg ctgggtcgta ttaaaggtta tctgactgga aagtgccgca
    14101 aggagtaata acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc
    14161 tttcatactg atgatgtatt agtactgatg agcggtcgcg ttgttaccaa agccaacctg
    14221 agtgacgtta acgctggagt tagatgcagt ctggtcaaca gcagcaccgt taccgccacc
    14281 gaactgttta acagtcatag tagaatcttt accattccac tgatcaagag tagcgctgtt
    14341 gccgaaacca cgctgagtca gatcgattga gctgtcatca gaaccttggc ccacatctgc
    14401 gccattaccg ccgccgtgct gggtaatggt cagatcagag ttacgggcgt cagcttgcag
    14461 agcaagagca gagttaccgc caccgtactg gtaaatattc agctctgaat tcgggccgct
    14521 gttattaccg ccaccaccgt ggttgccacc gccgccgccg tactgaggaa caacacctgc
    14581 cagagcgcta ccagagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta
    14641 aaacccccat cggattgatt taaaagtcga atggaaatta acgttgtgtc acgcgaatag
    14701 ccatttgcga ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat
    14761 ttgctttatt accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg
    14821 aaatactggc atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg
    14881 tctgatcaat ctttgcccgg ttgctactac cttcttgcgc aacaaccgcc aaaagttttg
    14941 agcctccctg ccgtaactga gcactattat tagtcccagc ttgaccaatt atggctgcct
    15001 gattaaatga agacttactc aattcattta ccgcaaagtt atattctgaa ttagctaaat
    15061 cataacctgc tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca
    15121 atttgttttt catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc
    15181 aacttggttg ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc
    15241 tgcggaaata tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca
    15301 aagtattaaa acccacgtaa atacgctgtt atctacgcaa aaatattttg ttttctttta
    15361 aatctccgtt ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat
    15421 tgctgcgata tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaatt
    15481 gtccacggtg gtatggagaa aaaacaagaa cgtttttcat gacgaaagga ctacagcgaa
    15541 atatttttta tatgcat
//
LOCUS       DADTFG010000117        15521 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.118, whole genome shotgun sequence.
ACCESSION   DADTFG010000117 DADTFG010000000
VERSION     DADTFG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.118"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..360)
                     /locus_tag="J1138_003410"
     CDS             complement(<1..360)
                     /locus_tag="J1138_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455171.1"
                     /translation="MSLSQFSSGRTPSNVLVNSWCNALNEAISTSATICAEDLYKGGH
                     WATAKAILDDYPIDLWILSAGLGLLHHKDNVVPYKATFAVGYDESIPLYSQEYVGKSF
                     HRTWWKEITNRSIFKSKH"
     gene            complement(859..2103)
                     /locus_tag="J1138_003411"
     CDS             complement(859..2103)
                     /locus_tag="J1138_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8455172.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGSGMHLLVHPNGSKYWRLQYRF
                     DGKQKMLALGVYPDVSLADARTRRDDARNLLANNIDPGDKKKSDKIEQEEARTFEQLA
                     VEWHATNKKWSEEHSRRVLKSLEDNLFPAIGKRNIADLKTRDLLAPIKSVELSGRLEV
                     ASRLQQRTTAIMRYAVQSGLLDYNPAQEMVGAVASSNRQHRPALQLKRIPELLRRIDS
                     YTGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETSMWTIPAEREAIDGVKHSQRGS
                     KMRTPHLVPLSHQALAILKDVYKLSGDRDFVFIGDHDHRKPMSENTVNKALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLDERRLML
                     QWWADFLDANRESEVSPYDFGK"
     gene            complement(2290..2374)
                     /locus_tag="J1138_003412"
     tRNA            complement(2290..2374)
                     /locus_tag="J1138_003412"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2338..2340),aa:Leu,seq:caa)
     gene            2570..3589
                     /gene="ahr"
                     /locus_tag="J1138_003413"
     CDS             2570..3589
                     /gene="ahr"
                     /locus_tag="J1138_003413"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBA8455173.1"
                     /translation="MSMIKSYAAKEAGGELEIYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADL"
     gene            3719..5221
                     /locus_tag="J1138_003414"
     CDS             3719..5221
                     /locus_tag="J1138_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBA8455174.1"
                     /translation="MSEPLLIARTPDTELFLLPAMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTTSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGTAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPTFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSSVYGKYEDDVDRESAYEMLQKGFQASIEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5382..6464)
                     /gene="lptG"
                     /locus_tag="J1138_003415"
     CDS             complement(5382..6464)
                     /gene="lptG"
                     /locus_tag="J1138_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBA8455175.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6464..7564)
                     /gene="lptF"
                     /locus_tag="J1138_003416"
     CDS             complement(6464..7564)
                     /gene="lptF"
                     /locus_tag="J1138_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBA8455176.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7831..9342
                     /gene="pepA"
                     /locus_tag="J1138_003417"
     CDS             7831..9342
                     /gene="pepA"
                     /locus_tag="J1138_003417"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBA8455177.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9600..10043
                     /gene="holC"
                     /locus_tag="J1138_003418"
     CDS             9600..10043
                     /gene="holC"
                     /locus_tag="J1138_003418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBA8455178.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10043..12898
                     /gene="valS"
                     /locus_tag="J1138_003419"
     CDS             10043..12898
                     /gene="valS"
                     /locus_tag="J1138_003419"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBA8455179.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12945..14132)
                     /locus_tag="J1138_003420"
     CDS             complement(12945..14132)
                     /locus_tag="J1138_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HBA8455180.1"
                     /translation="MNDVNIGKDNSRHSFVFTGKGGEYFLICLVNFLLTIITLGIYGP
                     WALIKCRRYIYQHVTLKGQPFSYKGTGGAIFVSMLLIVAVYLFSISCFAGQYFALGLF
                     LFALLICGIPCMAVKSLQYQANMTSLNGIRFGFNCSMLRAWWVMLGLPVLLALVFWFA
                     LYLIAQVTTSIGGLFFNLVALSLLSAIGLGVVHGITYSKWMPLLGNNATFGIHKFSIQ
                     VNVKECIKGCMLAILTMVPFIIVIGIMIAPVFQQLMMMTMLGRSDAGSEFVLQYYPQI
                     MASYFLYFVAILVFASYLYVTLRNLFLNNLTLANGTIRFHSSVTAIGMLLRMLAVLMG
                     SSITCGLAYPWLKMWMVSWIANNTHVQGDLDSLELTNDDKPQDSGSLMWISRGIMPYV
                     PFI"
     gene            14325..14828
                     /locus_tag="J1138_003421"
     CDS             14325..14828
                     /locus_tag="J1138_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA8455181.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYKHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            complement(15005..15421)
                     /gene="rraB"
                     /locus_tag="J1138_003422"
     CDS             complement(15005..15421)
                     /gene="rraB"
                     /locus_tag="J1138_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBA8455182.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
BASE COUNT         3983 a         3925 c         3976 g         3637 t
ORIGIN      
        1 atgcttagat ttaaaaatag atctattggt tatttctttc caccatgttc tatgaaaact
       61 tttccctaca tattcttgag aatataatgg tattgattcg tcatatccaa cagcaaaggt
      121 cgctttataa ggtacaacat tgtctttgtg gtgaaggagt cctaaaccag cagaaagaat
      181 ccataaatca ataggataat catctaagat ggctttagcc gtcgcccagt gcccaccttt
      241 ataaagatcc tctgcacata tagttgcaga tgtagaaata gcttcattca gagcattgca
      301 ccaagaattc acgagcacat ttgaaggggt tcggcctgat gaaaattgac tcagactcaa
      361 tatatcccta ctaccgtagt gtttgccgtt tgtacatgtg gttattaagt tgatgttgct
      421 cacaatcgcc cccccagaat cgtgttacac aagctacagg agaaaaaagg ggatgacaag
      481 aacggagtaa tcagtccctg aaaatttttt cagggactga tgttagtgag gaatatactc
      541 gaaatactca tcttgagaat gagaacatct ttggcatcgc ctcagaatac ctttttcctc
      601 aagagagaga caaaaatttc gacacgactc aagaaggtcg tcaggatttt catttttata
      661 tttagaaatc aacgctctag ctagggtgtc ataactaaaa cctcccgttg agtgcgatac
      721 aatgctcatc gcaagttgag tatcgaaagt tttcatagtg cacctctgag tcgccagaat
      781 aatacagtta taaaaaagta taggttatag ttcaagccgg cttttcaaaa tcgaacatag
      841 caagcatcag gaattagact atttgccaaa gtcataaggg ctaacttcgc tttccctatt
      901 cgcatccaaa aaatcagccc accactgcag catcagtcgc cgttcatcaa gatgttcggc
      961 cttatgaatg taagctgcgc gtactgagtt acgttccata tggctcatct gcctttccac
     1021 tgcatccctc gaccacaatc cagactcgat caatgaacta caggccattg tcctgaaacc
     1081 gtgcccacaa acctctacct tcgtgtcata acccataacg cgcagcgcct tgttcaccgt
     1141 gttttcactc atcggtttgc gatggtcatg atcgccgatg aaaacaaaat cgcgatcacc
     1201 actaagtttg taaacatctt taagaattgc taaagcttga tgagaaagcg gcaccagatg
     1261 aggcgtacgc atttttgaac cacgctgtga atgctttact ccgtcaatag cttcgcgctc
     1321 tgcaggtatt gtccacattg atgtttcgaa atcgatctca gaccaacgag caaagcgtag
     1381 ttcacttgaa cgaatgaata ccaggagcgt gagttcgacg gcaagtctag taagaggtct
     1441 accggtataa ctatctatgc gccgtaatag ttctgggatt cgcttcaact gaagagcagg
     1501 tcgatgctgt ctgttacttg aagcaacagc gccaaccatc tcctgcgcgg ggttatagtc
     1561 cagtaaacca ctctgcacgg catagcgcat aattgccgtg gttcgctgtt gtaatcgtga
     1621 agcaacctca agccgtcctg acagttcaac ggatttgatt ggggctagca ggtctcgggt
     1681 tttgagatcc gcaatattcc gcttaccaat agctggaaac agattatcct ccaggctttt
     1741 cagtactcgc ctgctatgtt cttccgacca ctttttattt gttgcatgcc actcaactgc
     1801 gagttgttca aaagtgcggg cttcttcttg ctcaatttta tcacttttct ttttgtctcc
     1861 cgggtcgatg ttgtttgcca acaaattacg agcgtcgtca cggcgagtcc gtgcatcggc
     1921 aagcgacacg tcaggataaa cacctaaagc cagcatcttt tgcttgccgt caaaacggta
     1981 ctgcaaacgc cagtacttag aaccattagg gtggacaaga agatgcatac cactgccatc
     2041 agttaacttg tattgcttat ctgttggctt agctgttctg actttgatat ctgttagagc
     2101 catgcttatc ccctccctgt tggtacaagc attatcgaac cggaaatacc atcatctgta
     2161 ccaacatctg ttggtagatg tacgttgaag tcggttgacc ttgagagagt taatatagcg
     2221 ggaatgatgg gtaaatactg gatttcaggc acaaaaaaag acgtccgttg acgtctattg
     2281 atgttccgat ggtgccgaag gccggactcg aaccggcacg tatttctacg gttgattttg
     2341 aatcaactgc gtctaccgat ttcgccactt cggcactgaa gaggtatgcg gaaaacgttg
     2401 tggattatac ctgtcgcacg ccaccatgca agcgccagct tgctcgcacc tcgctaagtg
     2461 ctgaaatttt cagcattaca ctcagcgatg tgtaaccttt gtcacactcc aggcaccccg
     2521 ccctgccatg ctctacactt cccagacaac accagagaag gaccaaaaaa tgtcgatgat
     2581 aaaaagctat gccgcaaaag aagcgggcgg cgaactggaa atttatgagt acgatcccgg
     2641 tgagctgaag ccacaagatg ttgaagtgca ggtggattac tgcgggatct gccattccga
     2701 tctgtcgatg atcgataacg aatggggatt ttcacaatat ccgctggttg ccgggcatga
     2761 ggtgattggt cgcgtggtgg cgctcgggag cgccgcgcag gataaaggtt tgcaggtcgg
     2821 tcagcgtgtc gggattggct ggacggcgcg tagctgcggg cattgcgatg cctgtattag
     2881 cggtaatcag atcaactgcg agcaaggtgc ggtgccgacg attatgaatc gcggtggctt
     2941 tgccgagaag ttgcgtgcgg actggcaatg ggtgattcca ctaccagaaa atattgatat
     3001 cgagtccgcc gggccgctgt tgtgcggcgg tatcacggtc tttaaaccac tgttgatgca
     3061 ccatatcact gctaccagtc gcgttggggt aattggtatt ggcgggctgg ggcatatcgc
     3121 tataaaactt ctgcacgcaa tgggatgcga ggtgacagcc tttagttcaa atccggcgaa
     3181 agagcaggaa gtactggcga tgggtgccga taaagtggtg aatagccgcg atccacaggc
     3241 actgaaagca ctggcggggc agtttgatct cattatcaac accgtcaacg tcagcctcga
     3301 ctggcagcct tattttgagg cgctgacgta tggcggtaat ttccatacgg tcggtgcggt
     3361 tctcacgccg ctgtctgttc cggcctttac gttaattgcg ggcgatcgca gtgtctctgg
     3421 ctctgctacc ggcacgcctt atgagttgcg taagctgatg cgctttgccg cccgcagcaa
     3481 ggttgcgccg actaccgaac tgttcccgat gtcgaaaatt aacgacgcca tccagcatgt
     3541 gcgggatggt aaggcgcgtt atcgcgtggt tctgaaagcc gatttatgat ccctttgccg
     3601 ggtgcctgtc gcccggcact taaatctctt tggaaggcgt ctcgcaatct gctgaattta
     3661 gcggtgaata gtgcgcagcc gttgcctata ctccaggcag gaaactggag gaaatctcat
     3721 gagcgaaccc ctgttaattg cccgcacgcc ggacacagaa ctgtttttac tacccgctat
     3781 ggctaaccgt cacggactga tcactggcgc aacggggaca ggtaaaactg tcacgctgca
     3841 aaaactggca gaatctctgt cagaaatcgg tgttccggtg tttatggccg atgtgaaagg
     3901 cgatctgacc ggcatcgcgc aggcaggaac gacctcggaa aaactgctcg caaggcttaa
     3961 aaatatcggt gtcaatgact ggcaaccgca tgccaatccg gtggtggtgt gggatatctt
     4021 tggcgagaaa ggccatccgg tgcgggcgac ggtttcggat ctggggccgc tgttgctggc
     4081 acgactgttg aatctcaacg atgtgcaatc tggcgtgctg aatatcattt tccgcattgc
     4141 tgacgatcag ggattgttgc tgctcgactt taaagatctg cgggcaatta cccagtacat
     4201 cggcgataac gccaaatcct tccagaatca gtacggtaat atcagtagtg catcggttgg
     4261 tgccatccag cgcgggctgt tgtcgctgga acagcaaggc acagcacact tctttggtga
     4321 gccgatgctg gatatcaaag actggatgcg caccgatgcc aacggtaaag gcgttatcaa
     4381 tatccttagc gccgaaaaac tctaccagat gccgaaactg tacgccgcca gcctgttgtg
     4441 gatgctttca gagttgtatg aacaattgcc ggaagcgggc gatctggaga aaccaaaact
     4501 ggtgtttttc ttcgacgaag cgcatctgct gtttaacgat gcaccgcagg tactgctgga
     4561 taagattgag caggtgataa ggcttattcg ctcaaaaggc gtgggcgtct ggttcgtttc
     4621 gcaaaacccg tcagatatcc cggataacgt gctcgggcag ctcggtaatc gcgttcaaca
     4681 cgctttgcgt gcttttacgc ctaaagatca gaaagcagtg aaggcagcgg cgcaaaccat
     4741 gcgggccaat ccgacgtttg ataccgaaaa ggcaatccag gaactgggga cgggcgaagc
     4801 gttaatctcg tttctcgatg cgaaaggaag tccttctgtg gtggaacggg cgatggtgat
     4861 tgcgccttgt tcgcggatgg ggccggtgac ggaagatgag cgcaatggct tgattaacca
     4921 ctcctcggtg tatggcaagt acgaggatga cgtggaccgg gaatccgcct atgagatgtt
     4981 gcaaaaaggc tttcaggcca gtatcgagca gcaaaataat cctcccgcga aagggaaaga
     5041 ggtggcggtg gatgacggta ttctcggtgg attgaaggat attttgtttg gcactaccgg
     5101 accacgcggc gggaagaaag atggtgtggt gcaaacaatg gcaaaaagcg ccgcccgcca
     5161 ggtgacgaat cagattgtgc gcgggatgtt ggggagtttg ctggggggga gaagaaggta
     5221 agccaggtgc gatttgtctt tgcctgatgc gacgctggcg cgccgcatca ggcctacact
     5281 actgcaatat attgaatttt catgatcttt caggcatgaa cgaaacacac tttgctaaca
     5341 ataataaagg gagctttcgc tccctttatt cgttcattcg gttacgattt tctcattaac
     5401 agccacaggc tgattaagaa gaagctggcg cttggcaaca gtgcgccgat gatcggcggg
     5461 atgccgtaaa ccaacgtcag cgggccgaag atctggtcca gtacgtagaa gacaaaaccg
     5521 aagctaatac cagtgaccac acgcacgccc atcggtacgc tacgcagtgg gccaaagatg
     5581 aacgacagcg ccatcagcat catcaccgcc acggatagcg gctggaagat tttgctccac
     5641 atgttgagct gataacgtcc ggcatcctga ccgctcgact tcagatactt cacatagttg
     5701 tgcaaaccgc tgatagagag tgcatccggg tccagcgcca ccacgcccag tttgtctggc
     5761 gtgaggttgg ttttccaggt gccgctcacc gtctgcgaac cggtgatctg tttcggattg
     5821 gtcaaatcag attcatcaac ctgcgacaga cgccagactt tatgttctgg gtcaaacttc
     5881 gcggtagcgg catagcgtac ggattgcaga cgacgattct cgttaaaggc ataaatactg
     5941 atgccaccta actcttcgtc acctttaacc cgctcaatgt agacgaagtt gttgccatct
     6001 ttcgcccata aaccttgctg ggtagagagc aacgagccgc cgtacatcgc ctgcgcacgg
     6061 tagttacgcg ccatctgctc gccctgcggc gcgacccatt caccaatcgc catcgtcagc
     6121 aagaccagcg gaatggcggt tttcatcacc gacagcgcca cctgcatacg ggtaaaacca
     6181 gaagcctgca tcaccaccag ttcgctgcgc tgcgccagca tcccaagacc aagcaacgcc
     6241 ccaagcagag ccgccatcgg gaagaagatt tgcacatctt tcggcacgct cagcaaggta
     6301 tacattcctg cgcctaacgc gtcgtaactc ccctgcccgg cttttttcag ctgatcgaca
     6361 aacttgataa tgccggacag cgacaccagc atgaacagcg tcatcatgat ggtggtgaaa
     6421 atagttttac cgatatagcg gtcaagtacg ccaaaaggtt gcatcacacc gctcctttac
     6481 gcgaaaaact ggcgcgcagg cggcggaccg gcacggtgtc ccaaaggttg agaacaatcg
     6541 ccaaagccag ataaatcagg ttaacggtcc acatccacag cgtcgggtcc agcttacctt
     6601 taccgccgtt cgatttcagg gaggtctgga tcaggaagaa caacagatac agcagcatgg
     6661 ctggcagcat cgatagtacg cgcccctgac gcgggttaac cacgctcagc ggtacgacca
     6721 taagtgccat cataaacacg gtgaacacca acgtgatacg ccagttcagt tctgcgcgag
     6781 cacgatcggt gtcagtgttc cacaatgtgc gcatgtccat ctggtcggta tcgttcgggt
     6841 cgagcgccac cgcctggtga ccaatgatcg cctgataatc ctggaaatcc gtaatgcgga
     6901 aatcacgtaa cagtgcagtg ccttcgaagc gcgttccctg gttgagagtg acgacctggg
     6961 agccgtcgcg cagctgggtt aaatgtccgg aatcggccac caccacagaa ggtcgtgcat
     7021 taccttttgg tcgaatttgc gcgaggaaca catctttgaa atcgctgccg tcaacgcttt
     7081 cgatgaacag caccgagctg ccattagtcg cttgctggaa ttgcccttgc gccagcgccg
     7141 ccatgccagg gttcgctttc gcttctgcta acacttcatc ctgatgacgc gatgaccatg
     7201 gtcccgccca catcacgtta accgccgcga cgattgccgt gaacaccgca aggatcattg
     7261 ccgctttcac cagcaccgct ttgctcagac cgcaggcatg cattaccgta atttcacttt
     7321 cggtatacag tttgcccagc gtcatcagca gcccgaggaa caggcttaat ggcaggataa
     7381 gctgcgccat ttccggcacg cccaacccga gaagggagag caccagattc gccggaatat
     7441 cgccgtcaac cgctgcgccg aggatcctca ctaacttttg acagaagaag atcaaaagca
     7501 agatgaagag tatcgccagc tggcttttga gcgtctcccg caccagatat cttatgatta
     7561 tcactttaaa tacgcccgta aaaactcgtc ttttgcagga ttttagcttg tttcatggct
     7621 taaacgtcat ttattctctt gagtcgtcga aatcgtcgct aagataatta tactcaacgg
     7681 attcacctct cagattttgt tctgacgtgc caatgccgta ataacgttaa gattaacacg
     7741 aagtcatcgc aacagcggac atgagttacg aaagcttgca attctatctg tagccaccgc
     7801 cgttgtcttt aagattcagg agcgtagtgc atggagttta gtgtaaaaag cggtagcccg
     7861 gagaaacagc ggagtgcctg catcgtcgtg ggcgtcttcg aaccacgtcg cctttctccg
     7921 attgcagaac agctcgataa aatcagcgat gggtacatca gcgccctgct acgtcggggc
     7981 gaactggaag gaaaaccggg gcagacattg ttgctgcacc atgtgccgaa tgtactttcc
     8041 gagcgaattc tccttattgg ttgcggcaaa gaacgtgagc tggatgagcg tcagtacaag
     8101 caggttattc agaaaaccat taatacgctg aatgatactg gctcaatgga agcggtctgc
     8161 tttctgactg aactgcacgt taaaggccgt aacaactact ggaaagtgcg tcaggctgtc
     8221 gagacggcaa aagagacgct ctacagtttc gatcagttga aaacgaacaa gagcgagccg
     8281 cgtcgtccgc tgcgtaaaat ggtgttcaac gtgccgaccc gccgtgagct gaccagcggt
     8341 gaacgcgcga tccagcatgg tctggcgatt gccgcaggga ttaaagccgc gaaagatctc
     8401 ggcaatatgc caccgaatat ctgtaacgct gcttacctcg cctctcaggc gcgccagttg
     8461 gctgacagct acagcaagaa tgtcatcacc cgcgttatcg gcgaacagca gatgaaagag
     8521 ctggggatgc attcctatct ggctgtcggt cagggttcgc aaaacgaatc gctgatgtca
     8581 gtgattgagt acaaaggcaa cgcgtcggaa gatgcacgcc caatcgtgct ggtgggtaaa
     8641 ggtttaacct tcgactccgg cggtatctcg atcaagcctt cagaaggcat ggatgagatg
     8701 aagtacgata tgtgcggtgc ggcagcggtc tacggcgtga tgcgtatggt cgcggagcta
     8761 caactgccga ttaacgttat cggcgtgctg gcaggctgcg aaaacatgcc tggcggtcgt
     8821 gcctatcgtc cgggcgatgt gttaaccacc atgtccggtc aaaccgtaga agtgctgaat
     8881 accgacgccg aaggccgcct ggtactgtgc gatgtgttaa cttacgttga gcgttttgag
     8941 ccggaagcgg tgattgacgt ggcgacgctg accggtgcct gcgtgatcgc gctaggccat
     9001 cacattaccg gtctgatggc gaaccataac ccactggccc atgaactgat tgccgcgtct
     9061 gaacaatccg gtgaccgtgc atggcgctta cctctgggcg acgagtacca ggaacaactg
     9121 gagtccaatt ttgccgatat ggcgaacatt ggcggtcgtc ctggtggggc gattaccgca
     9181 ggttgcttcc tgtcgcgctt tacccgtaag tataactggg cgcacctaga tatcgccggt
     9241 actgcctggc gttctggtaa ggcgaaaggc gcaaccggtc gcccggtggc gttgctggca
     9301 cagttcctgc taaaccgcgc cgggtttaac ggcgaagagt aattgcatca ggctgtgttg
     9361 ttattgtcgg atgcggcgtg aacgccttat ccgacctaca cagcactgaa ctcgtaggcc
     9421 tgataagacg cgacagcgtc gcatcaggca aggctgttat tgtcggatgc gacgtgaacg
     9481 ccttatccga cctacacaca gcacttaact tgtaggcctg ataagacgcg gcagcgtcgc
     9541 atcaggcgct gcggtgtata cctgatgcgt atttaaatcc accacaagaa gccccatata
     9601 tgaaaaacgc aacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg
     9661 ttgagcagtt ggtgtgtgaa attgccgcag aacgttggcg cagtggtaag cgcgtactca
     9721 tcgcctgtga agatgaaaag caggcttacc gactggatga agccctgtgg gcgcgtccgg
     9781 cagaaagctt tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg
     9841 agatcgcctg gccgcaaaag cgcagcagca gcccgcgcga tatattgatt agtctgcgaa
     9901 caagctttgc agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag
     9961 attctctgaa acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc
    10021 tgaatacggc aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc
    10081 gctttacgag cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga
    10141 aagtttctgc atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc
    10201 cttccagcaa accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac
    10261 cctgtggcag gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa
    10321 gattgccgca gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa
    10381 aatctgggaa tggaaagcgg aatccggcgg caccattacc cgtcagatgc gccgtctcgg
    10441 caactccgtc gactgggagc gtgaacgctt caccatggac gaaggcctgt ccaatgcggt
    10501 gaaagaagtt ttcgttcgtc tgtataaaga agacctgatt taccgtggca aacgcctggt
    10561 aaactgggac ccgaaactgc gcaccgctat ctccgacctg gaagtggaaa accgcgaatc
    10621 gaaaggttcg atgtggcaca tccgctatcc gctggctgac ggcgcgaaaa ccgcagacgg
    10681 taaagattat ctggtggttg cgactacccg tccggaaacc ctgctgggcg atactggcgt
    10741 ggccgttaac ccggaagatc cgcgttataa agatctgatt ggcaaatatg tcattctgcc
    10801 gctggttaac cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac
    10861 cggctgcgtg aagatcaccc cagcgcacga ctttaacgac tatgaagtcg gtaaacgtca
    10921 cgccctgccg atgatcaaca tcctgacctt tgacggcgat atccgtgaaa gcgcccaggt
    10981 gttcgatacc aaaggtaacg aatctgacgt ttattccagc gaaatcccgg cagagttcca
    11041 gaaactggag cgttttgctg cacgtaaagc cgtcgttgct gcggttgacg cgctcggcct
    11101 gctggaagaa attaaaccgc acgacctgac cgttccttat ggcgaccgtg gtggcgtggt
    11161 tatcgaaccg atgctgaccg accagtggta cgtgcgtgcc gatgtactgg cgaaaccggc
    11221 agttgaagcg gttgagaatg gcgacattca gttcgtaccg aagcagtacg aaaacatgta
    11281 tttctcctgg atgcgcgata ttcaggactg gtgtatctct cgtcagctat ggtggggtca
    11341 ccgtatcccg gcatggtatg acgaagcggg taatgtttat gttggccgca acgaagacga
    11401 agtgcgtaaa gaaaataacc tcggtgctga tgttgcgctg cgtcaggatg aagacgttct
    11461 cgacacctgg ttctcctccg cgctgtggac cttctccacg ctcggctggc cggaaaacac
    11521 cgacgcactg cgccagttcc acccaaccag cgtgatggta tccggcttcg acatcatctt
    11581 cttctggatt gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa
    11641 accgcaggtg ccgttccaca ccgtttacat gaccggcctg attcgtgatg acgaaggcca
    11701 gaagatgtcc aaatccaaag gtaacgtgat tgacccacta gatatggttg acggtatttc
    11761 gctgccggaa ctgctggaaa aacgtaccgg caatatgatg cagccgcagc tggcggacaa
    11821 aatccgtaag cgcaccgaga agcagttccc gaacggcatt gagccgcacg gtactgacgc
    11881 gctgcgcttc accctggcgg cgctggcttc taccggtcgt gacatcaact gggatatgaa
    11941 gcgtctggaa ggttaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct
    12001 gatgaatacg gaaggtcagg attgcggctt taacggcggt gaaatgacgc tgtcgctggc
    12061 ggatcgctgg attctggcag agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga
    12121 cagcttccgc ttcgatatcg ccgcaggcat tctgtatgag ttcacctgga accagttctg
    12181 cgactggtat ctggagctga ccaagccggt aatgaacggt ggaacagaag ccgaactgcg
    12241 cggtactcgc catacgctgg tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat
    12301 cattccgttc atcaccgaaa ccatctggca gcgagtgaaa gtactttgcg gtatcactgc
    12361 cgataccatc atgctgcagc cgttcccgca gtacgatgcg tctcaggttg atgaagccgc
    12421 actggccgac accgagtggc tgaaacaggc gatcgttgct gtacgtaaca tccgtgcaga
    12481 aatgaacatc gcaccgggta aaccgctgga gctgctgctg cgtggttgca gcgcggatgc
    12541 agaacgtcgc gtaaatgaaa accgcggctt cctgcaaacg ctggcgcgtc tggaaagcat
    12601 caccgtgctg cctgctgatg ataaaggtcc ggtttccgtt acaaagatcg tcgacggcgc
    12661 agaactgctg atcccgatgg caggcctcat caacaaagaa gatgagctgg cacgtctggc
    12721 gaaagaagta gcgaagatcg aaggcgaaat cagccgtatt gagaacaagc tggcgaacga
    12781 aggctttgtc gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaga agctggaagg
    12841 ctatgcagaa gcgaaagcga agctgattga acagcaggct gttatcgccg cgctgtaata
    12901 ctcatagtga aaacgaaggc cggagcatgc tccggccttt ttatttagat aaacggaaca
    12961 tacggcataa taccgcgaga tatccacatt aacgaaccgc tatcctgcgg tttgtcatca
    13021 ttggttaact ccagcgaatc aaggtcgccc tggacatggg tattgttagc aatccagcta
    13081 accatccaca ttttcagcca gggataagcc agaccacagg taattgagga ccccattaat
    13141 accgccaaca tgcgcagaag cataccgatc gcggtaacag atgaatgaaa acgaattgtc
    13201 ccatttgcca gcgtcagatt attcaaaaac agattgcgta acgtgacata gagataactg
    13261 gcaaaaacaa ggatcgcgac aaaatacagg aaataagaag ccataatctg cgggtaatat
    13321 tgtaatacaa actcactgcc agcatcagac ctacccaaca tggtcatcat cattaactgc
    13381 tggaatactg gtgcgatcat aataccaatc acaatgatga aaggaaccat ggttaatatt
    13441 gcgagcatgc aacctttaat gcattctttt acgtttacct gaatagaaaa cttatgaatg
    13501 ccaaaagttg cattattacc cagcaatggc atccatttac tataggttat accatggaca
    13561 acacctagcc cgatggcaga aagcaaactt aatgcgacta agttaaagaa taaccctcct
    13621 attgatgtcg tcacttgtgc aataagatac agtgcgaacc aaaaaactaa cgccaacaga
    13681 acaggcagtc ctagcataac ccaccatgca cggagcattg agcaattaaa gccaaagcga
    13741 atgccattta gtgaagtcat attcgcctga tactgtaggc tcttcactgc catacaagga
    13801 ataccgcaga tcaagagagc aaacaggaat aaaccgagcg caaagtattg acctgcaaaa
    13861 caagaaatac tgaaaagata aaccgccaca ataagtaaca tgctaacaaa aatggcacca
    13921 cctgttccct tataactaaa tggttgcccc ttgagtgtca catgttgata aatataacga
    13981 cggcatttta tcaatgccca cggcccatat attcccagag taataattgt cagtaaaaaa
    14041 ttcacaagac aaataagaaa atactcacca cctttgcctg tgaaaacaaa cgagtgccga
    14101 gaattatctt tcccgatgtt tacatcattc atgaactata tccctaaaat aaattccatt
    14161 cataccgata ataattccag tcaattgata ctggttcgcc tagaatacgc tttatctcag
    14221 agagatatgc gtaatatttt ctgatttatc ttcttcttgc accacctaag cgagggtggt
    14281 attaaagtga gctataaatc atacatttga aattgagcta tgctatgaat gttgtcgctt
    14341 ctcccgcact ccgtttgcgc aaacttaccg tcgccgataa tcctgctatc gcccacgtca
    14401 ttcgtcaggt atccgccgaa tacggtctta ccgctgataa aggctacacc gtcgccgatc
    14461 cgaaccttga cgagctgtat caagtatata gtcagcctgg ccacgcatat tgggtggttg
    14521 agtacgaggg tgaagtggtc ggcggcggtg ggattgcgcc attaaccggg agtgagtcag
    14581 acatttgcga actgcaaaag atgtattttc ttccggctat ccgcggcaaa gggctggcaa
    14641 aaaaactggc cttaatggca atggagcagg cgcgagaaat gggtttcaaa cgctgctatc
    14701 tggaaacgac cgctttttta aaggaagcca ttgcgcttta taagcatttg ggctttgagc
    14761 atatcgacta tgcgcttggc tgcacgggtc atgtcgattg tgaagtacgg atgctacgca
    14821 aactttgaaa aatgcccgcg agcttagact cgcgggcatt cgaacattaa ccacgtgctt
    14881 tttgagtatg caggatgtga cgtaaatgcc ttatccgggc tacaaattca ccaggttgca
    14941 ggaactacct gggcctgata tgcgtagcgc atcaggtaac gcagcctttg tcgtcaacgc
    15001 ttaattagtg acgaataccg tcatcgtctt catcgacaaa atcttcatcg tcgccgtctt
    15061 cgccgttagg atcttcaaag taagtgcccc aaccatcgta ctcaacgtca aatttctccg
    15121 ccagcgtcat cagttgttca acctgggcat cgatcaggtc ggcattcaac gcgcactcgc
    15181 tgaggatgtc gcagcaaatc acgatgtcac catcttcaac ttccagctct tctggatcgg
    15241 tcacttcgta accgagttta aacgcttcaa ctgctgcttt ttccagggtt tccagatcgt
    15301 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    15361 attcttcaat aatcaagcgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    15421 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    15481 tccacctttg tgacaaagat ttatgcttta gacttgcaaa t
//
LOCUS       DADTFG010000118        15324 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.119, whole genome shotgun sequence.
ACCESSION   DADTFG010000118 DADTFG010000000
VERSION     DADTFG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.119"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            281..1051
                     /locus_tag="J1138_003423"
     CDS             281..1051
                     /locus_tag="J1138_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBA8455183.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1042..1515
                     /locus_tag="J1138_003424"
     CDS             1042..1515
                     /locus_tag="J1138_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBA8455184.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1623..2162
                     /gene="dnaT"
                     /locus_tag="J1138_003425"
     CDS             1623..2162
                     /gene="dnaT"
                     /locus_tag="J1138_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBA8455185.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     ITPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2165..2902
                     /gene="dnaC"
                     /locus_tag="J1138_003426"
     CDS             2165..2902
                     /gene="dnaC"
                     /locus_tag="J1138_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBA8455186.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2951..3445
                     /locus_tag="J1138_003427"
     CDS             2951..3445
                     /locus_tag="J1138_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBA8455187.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3699..5990
                     /gene="opgB"
                     /locus_tag="J1138_003428"
     CDS             3699..5990
                     /gene="opgB"
                     /locus_tag="J1138_003428"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBA8455188.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6130..7152)
                     /locus_tag="J1138_003429"
     CDS             complement(6130..7152)
                     /locus_tag="J1138_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA8455189.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSLARREHVATRLELPVLDPLAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7291..8196
                     /locus_tag="J1138_003430"
     CDS             7291..8196
                     /locus_tag="J1138_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA8455190.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8367..10019)
                     /locus_tag="J1138_003431"
     CDS             complement(8367..10019)
                     /locus_tag="J1138_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBA8455191.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
     gene            10710..11681
                     /locus_tag="J1138_003432"
     CDS             10710..11681
                     /locus_tag="J1138_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HBA8455192.1"
                     /translation="MSKIIAMLITVSTFFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFGTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            11745..12218
                     /locus_tag="J1138_003433"
     CDS             11745..12218
                     /locus_tag="J1138_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBA8455193.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            12215..13486
                     /locus_tag="J1138_003434"
     CDS             12215..13486
                     /locus_tag="J1138_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBA8455194.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WHALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(13529..15193)
                     /gene="tsr"
                     /locus_tag="J1138_003435"
     CDS             complement(13529..15193)
                     /gene="tsr"
                     /locus_tag="J1138_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBA8455195.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDAGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQREASAVVKTVTPATPRKMAVADSEENWETF"
BASE COUNT         3960 a         3897 c         3596 g         3871 t
ORIGIN      
        1 tctgatggtt ttaacttgtt aacattaaaa aacgcgccca gctatctatt atcctgactc
       61 gtaaaggcca aataacaaat tcattatagt gataatattc atgcgatagt ttgtttacgg
      121 cgagagataa ttcgcttttt atcaccgagc atctcccgat aaccaggatt aatcagcgca
      181 attttgatct taacctaccg caaaacaacc gtgcttcatg atacaaatgg attctcatta
      241 tctgaacaat acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg
      301 agccgtaaca cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag
      361 cgcgttggtt gatgagcttt cctcacgact gggtcgggcg ttgggaatgg acagcgtcga
      421 aagttctatc tcttcgaacg ccatagtgct gacaaccatt aaagatgggc aatgcctgac
      481 atcgacacgt aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca
      541 cattgtaatt ctggcggagc atcatctgct ggattacaaa ggcgtagaga aacgatttag
      601 ccaaattcag ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcctg
      661 cgcctgtttc tgtaaactca ataaaggtgg ctgggatggt gccgtcatca ccttcctggc
      721 cagtacggcc gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccacagat
      781 caacttttgc ctcaccgctt tcgccgccac caccatttcc ggattgcttt tgcaacttcc
      841 cactttcagc aataccccca ccattgcgat ggccgccagc gttctgctgc tggtgccggg
      901 ctttccgttg ataaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc
      961 acgctgggca atcgccagtc tgctgacact ggctacctgt gtcggcgtag taatggcact
     1021 gacgatttgg gggctacgcg gatgggtgtg atcgaatttc tgttagcgtt ggcgcaggat
     1081 atgatcctcg ccgccattcc tgcggtcggc tttgcgatgg tgttcaacgt tcccgttcga
     1141 gcgttacgct ggtgtgcgct gcttggtgcg ataggtcatg gttcccgaat gatcttaatg
     1201 accagcgggc tgaatattga atggtcaacc tttatggcct ctatgctggt cggcaccatt
     1261 ggtattcaat ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggctgtt
     1321 atccctatgt tccctggcat atcagcttat accgcaatga tttcggcggt aaaaatcagc
     1381 cagctaggtt acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca
     1441 tcgattgttg gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag
     1501 cgccctcgcg tataaaattg cctcacttaa agcacgcgga tgagagacag cctcctctcc
     1561 tccgtgtgtt actataaaag ttatctccct tctcgttcat cgttccatat ttgagaaaca
     1621 gtatgtcttc cagagttttg accccggacg tcgttggtat tgacgccctg gtacacgatc
     1681 accaaaccgt tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc
     1741 cggcgtttta tgccatcact cctgcacgcc tggctgaact gctggcgctg gaagaaaagc
     1801 tggcgcgtcc gggaagtgac gtggccctgg acgatcaact ctatcaggaa ccgcaaaccg
     1861 ctcccgttgc cgtacccatg gggaaatttg ccatgtatcc ggactggcaa cccgatgccg
     1921 actttatccg cctggcggcg ctatggggcg tggcactaag agagccggtg accgccgaag
     1981 aactggcgtc attcattgcc tactggcagg cggaaggcaa agtctttcac catgtgcagt
     2041 ggcaacaaaa actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga
     2101 aacgagatgt gaatacggtc agtgaacctg acagccaaat tccaccagga ttcagagggt
     2161 aacgatgaaa aacgttggcg acctgatgca acgcctgcaa aaaatgatgc ctgcccatat
     2221 caaacctgcg ttcaaaacgg gtgaagaatt actggcctgg cagaaagaac aaggggcgat
     2281 ccgctccgcc gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg
     2341 ctccggtatt cgtccactgc atcagaactg ctcctttgag aactatcgcg ttgagtgtga
     2401 agggcaaatg aatgcgttaa gcaaagcgcg ccagtatgtc gaagagtttg acggcaacat
     2461 cgccagcttc atcttttctg gtaagccagg aaccggcaaa aaccatctgg cggcggcaat
     2521 ctgcaacgag ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg ccgatattat
     2581 gtcggcgatg aaagatacct tcaggaatag cggtaccagc gaagaacaac tgcttaacga
     2641 tctgagtaac gttgatctgc tggtgatcga tgagatcggc gtgcagaccg aatcgaaata
     2701 cgaaaaagtg atcattaacc agatcgtcga tcgccgctct tcttccaaac gcccaaccgg
     2761 gatgctgacc aacagtaata tggaagagat gaccaagttg ctgggcgaac gcgtgatgga
     2821 ccgtatgcgc ctgggtaaca gtttgtgggt gatctttaac tgggatagct accgtagccg
     2881 tgtaacaggt aaagagtatt aatccccgtt tctggcggta tactgcgaca caattcaacc
     2941 ttgagtaatg atgatgaaaa ctattaaaca tcttctgtgc tgtgccattg ccgccagcgc
     3001 attaatttcc accggggtgc atgctgcgtc ctggaaagat gcgctctcca gcgccgccag
     3061 cgaacttggc aaccaaaaca acacgacaca ggaaggcggt tggtcgctcg cgtcattaac
     3121 taacttgctt agcagcggga accaggcctt aagcgcagat aacatgaaca acgccgcagg
     3181 cattctgcaa tactgcgcga agcaaaagct ggcttcggta accgatgccg aaaacatcaa
     3241 gaatcaggta ctggaaaaac tgggcctgaa cagtgaagag caaaaagaag acaccaacta
     3301 tctggatggc attcagggtt tgctgaaaac caaagatggt cagcaactca atctggataa
     3361 tattggaacg actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa
     3421 gcaggggctg aacttcattt cctgaaccgc gaaacgccgc gttttagctg ttacccggct
     3481 gacgcggctc tctcctgtcc ttttccctgc tcctggacgg ttttacccct gttacacggg
     3541 aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc ctaacaacat cgcatttttt
     3601 tgacactaga attgcagcaa tatggcgacg ccattacatt gtgcttctca aaaagcttaa
     3661 acgttggcta aacggcccgg tttagtgagg atcgcccgtt gtcagaacta ctctctttcg
     3721 ccctttttct cgcctctgtg ctgatttacg catggaaagc gggacgtaac acctggtggt
     3781 ttgcagccac attaacggta ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca
     3841 gcgactattt tactggcgat ggtattaacg acgcggttct ctatacctta accaacagcc
     3901 tgaccggtgc tggtgtcagc aaatacatcc tgcctggtat cggcattgtg ctggggctgg
     3961 cggcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat ccgcaccatt
     4021 ttggttacag cctgctggca ctcttactgg cgctgggttc agtggatgcc agcccggcat
     4081 ttcgtcagat aacggaactg gtgaaatccc agtcacgcga cggcgacccg gactttgcgg
     4141 cttattataa agagccgtcg aaaactatcc ctgacccgaa actcaacctg gtttatatct
     4201 acggcgaaag tctcgagcgg acctattttg ataacgaggc tttcccggat ctcacgcctg
     4261 aactgggcgc gttgaaaaat gaaggcctgg atttcagcca cacgcagcag ctgccaggaa
     4321 cggattacac gattgcgggc atggtggctt ctcagtgcgg cataccgctg tttgccccat
     4381 ttgaaggcaa cgcctccgcc tctgtttcca gcttcttccc gcagaacatc tgtctgggcg
     4441 atatcctgaa aaactcgggt tatcagaact atttcgtgca gggcgcgaat ctgcgttttg
     4501 ccggtaaaga tgtgttcctg aaatcgcacg gcttcgacca cttatacggc tcagaagagc
     4561 tgaaaagcgt ggtggccgac ccgcactatc gcaacgactg gggattctac gacgataccg
     4621 ttctcgatga agcgtggaaa aagtttgaag agctttcccg ctcaggtcag cgattctcac
     4681 tgtttaccct gacagtcgat acccatcacc cggatggttt tatctcgcgt acctgtaacc
     4741 gcaaaaaata tgattttgac ggtaagccga atcagtcatt cagcgcggta agttgcagcc
     4801 aggagaatat tgcgacgttt atcaacaaaa tcaaagcgtc accgtggttt aaagataccg
     4861 tcatcgtcgt ctcttctgac catttagcga tgaacaatac ggcgtggaaa taccttaata
     4921 agcaggatcg caataacctg ttttttgtca tccgtggcga caagccgcag caagagacgc
     4981 tggcagtgaa gcgtaatacg atggataacg gcgcgacggt gctggacatt ctcggtggcg
     5041 ataactatct cggacttggc cgtagcagtt tatccgggca gtcaatgtcg gaaatcttcc
     5101 tcaatatcaa agagaaaaca ttggcgtgga agccggatat catccgcctg tggaaattcc
     5161 ctaaagagat gaaagagttc accatcgacc agcagaaaaa catgattgcc ttctcaggta
     5221 gccatttccg tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa ccgctgccgg
     5281 aaagcgaata ctcagcaccg ctgcgtttcc agctggcaga tttcgctcca cgcgacaatt
     5341 tcgtctgggt tgaccgttgc tacaagatgg cacaactctg ggctccggaa ctggcactct
     5401 ccaccgactg gtgtgtctcg caagggcagc ttggcggtca gcaaattgtt cagcatgttg
     5461 acaaagcaat atggaaggga aaaacggcat ttaaagatac ggtcatcgac atggcgcgtt
     5521 acaaaggcaa tgtcgatacg ctaaagattg ttgataacga tattcgctac aaagccgaca
     5581 gtttcatctt taacgtcgct ggtgcgccag aagaggtgaa acagtttagc gggatttctc
     5641 gtccagagtc gtggggccgc tggtccaacg cacagctggg cgatgaagta aaaatcgagt
     5701 acaagcatcc gctgccgaag aaatttgacc tggtgattac cgccaaagca tacggcaata
     5761 acgccagccg tcctattccg gtacgcgtag gcaatgaaga acaaaccctt gtgctgggca
     5821 atgaagtgac caccaccaca ctgcatttcg ataacccaac cgatgccgac acactggtaa
     5881 ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat cctcggacac tcgccgcgta
     5941 agctcgggat cggcatggtg gaaattaaag tggtagaacg tgaagggtaa ttcctgactg
     6001 atgtgtaggt cgcataaggt gcgacctaca caccttctcc ggcctacaaa acattattga
     6061 gaaacagaaa aaaaacagcg cccaacactt ccccggacgc tgcatggcaa ctttaataat
     6121 ctcgccttat cagaaagtaa ttacgccctt aatcaactca cgattgttaa tcacatcgcg
     6181 ctcgtaggtt tctgccagcg tggcgaacgg atagcggtgg gttaacatca tgtcggcagt
     6241 gattttccct tccgccatca atcgaccgac tttagcgaaa tcttccggcg tggcgttgcg
     6301 gctgcccatc atcgtcgttt cttttttatg gaattccgga tcggagaact gcaactcacc
     6361 tttaaacagg ccgacaaata ccaccgtgcc gccgtgacga atcagattta cggtgttatt
     6421 catcgcatgt tgattacctg tcgcgtcaat cactttctgc gccagcgaac caccaaactg
     6481 cgcccgcagc tgcgcgtcaa aatcttcggc taacgggtcc agaacaggta attccagacg
     6541 cgttgccaca tgttcacggc gcgccagact ggtatccgcc accaccacct gtgcgccatc
     6601 ggctttagcg attgccgccg cgcccagacc gattggcccc gccccgacca ccagcacctg
     6661 ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat
     6721 caatgctgcc gcctgcgggt caataccgtc tgcgggcaaa atgttcgcca ccggcacgct
     6781 caaatactca ctaaaaccgc catcctgatg cacgccaatg actgaaattt tttcacagca
     6841 attggtacga ccgcttttac acgccgggca ttgctgacaa gaaatatacg gaattaccgc
     6901 aacttgctgt ccgatattaa aattgtgaat gttttttcct gattcaataa tctccccaca
     6961 tatttcatgg cctaaaacgc gtggataact gaaaaatggc tgattgcccg cccaggcatg
     7021 aatatctgta ccgcaaatcc cgacagtttt aattttaatt aatgcttcat tatcgcctgg
     7081 caccggtctt tcccggactt cccatactaa attctttatt tcctgacaaa ttaatacttt
     7141 cattgtggac atgattcacc gctcctttta ttttatgact ttcagatatc gtcgaattta
     7201 taagagcgcc tgaatttacc cataaaatat gcggatgatc gcgcataaaa atgttttaaa
     7261 tcggctttta atgccggact ggagggaaga atgagtcgtt cgcagaattt acgtcatagc
     7321 gtcataaatc aggtgattga agatatggcg aggggcaata ttccttctcc gctgccttca
     7381 caaagcggac tggcagaaat gtataacatc agtcgcacta cggtacgcca catactccag
     7441 catttaagcg catgtggcgt gctcaccctg gtggggaaaa attatgttat cgcccgcaaa
     7501 cccgaacaga acgatggatt tgattgcatc acggcctcgc tgaaagagca aaaccgtctt
     7561 ttcgagcagg catttttcac catgattaac ctgcgccagt tacgcgccgg agaaagcttt
     7621 tccgaacttc agctggcgcg agcggcaagc gtcagtccgg tagtggtacg agaatatctt
     7681 ttgaaattcg gtcgttataa tcttattcaa agcgaaaagc gcggccaatg gagcatgaag
     7741 cagttcgatc aatcctacgc cgagcagctg tttgagctac gcgaaatgct cgaaacacat
     7801 tccctgcaac actttctcaa ccttcccgat gatgaccctc gctggttgca ggcgaaaacc
     7861 atgctcgaac gtcacaggac attgcgcgac agcattggca gcaactttcg aatgttttct
     7921 caattagacc gtgattttca tgccatgctg ttatccgctg ctgacaacat cttttttaat
     7981 caatcacttg aaattatatc ggtgatcttt cattttcact atcaatggga tgaaagcgat
     8041 ctcaaacagc gcaatatcat tgctgttgat gaacatatga ccattctcag cgccctgatt
     8101 tgccgtagcg atttagatgc aataacggcg ttgcgaaacc atctggatac cgccaaacaa
     8161 tcaatgattc gttccatcag gcaacatcac cgataaatta agtacagatt aaaaacattg
     8221 attcttggtt tatatcacta tgttgtatta atgcagtatt aatccaggcg attgttctgc
     8281 ccataaggaa atacataggg aataaacgat gctggaagta ttactctcag ccatcattta
     8341 attccctatt actggcagcg ataaaattat cggctatcca tgattaacgt ttccagcaca
     8401 tctaattcac tgactatttt tgccagtgtt tcatcactga cttcttgcct tgcgcgcatc
     8461 tggtaaatag ccgcgcgttt ggcatttaac gccgccagat taaatgttct ttccagcttc
     8521 gcacgctgca tatgcaggga attatcatta ggcgacatat cctcttcact atgtcgcata
     8581 taaccgataa ctctggcccc cgtttccata atcaactcgt tatcgaggtt ttctgttctg
     8641 gcgcgcagca aacgttgctc ggtcttttgc acactcagta tggccatttt agccataaac
     8701 agatcggcag cttcctcttc tttttgtcgg ttatcgctga aaatccccac atttttcccc
     8761 agtaataacg gcagaataat aatgccgcaa accagcgaaa agaggatcac acctgtggcg
     8821 agaaatacca gcaagtaacg cccgggaaaa ggtgtgccat ccggcagcaa taacgggata
     8881 gaaagcaccc ccgcaagcgt gatagctccg cgcacccccg caaaggtcgc cagcagcata
     8941 tgacgcgttt tatatccagc aaacagcatc ggatgtttgc cgggtcgttt acggctatac
     9001 agacgcatca gccaaagcca gcccaggcgc acaaccatta acgccaggta gatccaaatg
     9061 acatcaagga gcagcgatcc ggtgtccacc agtggatcgt tttctgcacg gatcagtgat
     9121 tccgaaataa tgcccggcag ttgtaagcct aaaatcagaa acaccatgcc gttaaagaca
     9181 tattcaagca tcgaccagct acttttagct ttaattctca ggcgaatagg cgcgtggtgg
     9241 actacgccaa tgcgactggt tgttaacccc gcggcgacag cggcaagaat gccagataag
     9301 ccaaaatgtt ccgcaaccag ataaacaata aacggcaaca gaagcatcaa tacaatctga
     9361 atggaaggtt catcgtgagc atatcggctg atcaacagca gccctttacc atataaccag
     9421 gtaacagcga tccccacggc aataccgccc aacgcaacga caaaaaacgc aatggttgcg
     9481 ccatgaacgc tgaattccat cgttcccatc gtcactgcaa cagcaaattt caacgccacc
     9541 aggccggaag catcattcat caacgcttcg ccttcgagca cttcctggat gtttttgctg
     9601 atacgccctt tcccgacaat ccccagcaac gccacggcat cggtaggcga caacacggcg
     9661 gctaatgcga aagcagcgat gagcggcaca ttcggtaaga gtaaatgaat cacatagccg
     9721 acaccaaaaa tagtgattag taccaacaca agtgccaggc tgataatttc ccggctgtgc
     9781 ttgatcaatt ctttaggttg tatttttgaa ctttcagcaa acaacagggg tggtataaac
     9841 aataagagaa aaagttcagg attgaattcc acatgcagac caaatatagg ctgggccagt
     9901 actgcgccag cggcaatctg gattaaagga agtggtatct ggacaggaga cagacgtgta
     9961 agcacgctgg ttaatgatac taccaacgta agaattaaaa ttgttaaaag tatttccata
    10021 taataaacag gcttcctcat tcataaataa tggatattat taatttaact tcataaaaag
    10081 cgctctggtt agcacttaac gttaatcatt aatcactgcg ttcactcata tgatcagaag
    10141 gaacccagta aaatgagttt tcaagttgat aacaaattca ccacgtgaat atttactgcc
    10201 tttccccgtt gtaacgaatc gtccgttact ctctgatttc cttgccgaac aagttattta
    10261 gtgaatgacg gctttctttt aatatcagca tgaattatac gttcagcaaa tgtattaaat
    10321 cagcactaaa taaatttaat ggataccacc attctgcgtc aacacttagt ttttatctgt
    10381 attgacaatt taaaatggcc gcagccaaat taatgatttt atttttaata aatcatcaac
    10441 agaataagag atcttatcag cggtaactaa actcatcaga gggtgttcct tgtaacgtca
    10501 tgcattacat aactttaaaa tatacaatca aaaaatgcat taaaaaccga ttaaaagcag
    10561 caaagtatta cccggtgcac gaatttatga tacatctgca gccattctgc tattcgctga
    10621 ctacttttca gtaatgactg ctttttcata aggtttcact cccgacaaca atgttggagt
    10681 tttattttct tagcatggag taagtaaaga tgagcaaaat aatagcgatg ctaattactg
    10741 tcagtacctt ttttttaccg tcaataatcc aggcaaaacc tatttcaata aaagttgcct
    10801 atgaaaataa ccctggagaa cccctggatg tagtcatgcg ttactgggca gacctattaa
    10861 ataaaaaaag taatggcgaa ataacacttg cgctctatcc cagctcccaa ttgggatcaa
    10921 aacaggatgt caccgagcaa gccatgatgg gcatgaatgt cataaccttg agtgatgtgg
    10981 cctttcttgc cgattatgaa cccgatttgg ggattttgtt tggaccgtat ctgactgacg
    11041 atccgcaaaa actgttcaaa atatatgaaa gcgactggtt taaacaaaaa aatgaaagtc
    11101 tgaagaaaaa gggaattcat gtggtaatga ataattatct ctatggcact cgacagatta
    11161 tttcgaaaaa acccattcgc accgttgacg atctcgcagg tttaaaaatt cgcgttccta
    11221 ataacgtcat gcaaataaaa gccattcagg cgatgggggc aacaccaact cccatgccat
    11281 taggcgaagt ttatcccgca ttaacccagg gcgttattga cggcgtagaa aacccaattt
    11341 cagtgctgca agggcaaaaa ctgtatgagc aggcaagata tctgacaatg gttaactacc
    11401 tgacaaatac ctcagtgtgg ataggtggtg aagccttctt tggcaccctt tcaccagaac
    11461 aactggagat gatccaccag accggctatg aagcgggtgt ctatagccag aaactcactc
    11521 ttgagcgtga tgccgaaatg ttgaaaacca tgcaggcagc cggcgtcgaa gtgatctatc
    11581 ccgatactgg accattccag aagaaagcac gcgaagttta ctctcaattc ccggaatgga
    11641 caccaggtct gtacgaaacc attcaacagc aactgcaata agcattaatg ctcagcgtcc
    11701 tgcctcatct ctcttaggca ggacacgcac taacaggagg tttcatgcgt ctttttggca
    11761 aagtggcaga atttagtgct gcctttgcgc tattcgttct ggttgtggtg acaattggag
    11821 cggtatttat gcgttatttc atcggccaac cgcttcaatg gacagaagaa atgtcgggaa
    11881 tgctgatgat ttgggtggtg atgttaggtg gagtggtggc ggaacgtgac agggcgcacc
    11941 tgaccattcc cttcctgatg gagatgcttc caggcaaact caggcgtgtt attgcagtac
    12001 tggtcgccct tctctccatc gcactgctgc tttatatggc ctggctgggc taccgactag
    12061 ccgaaatggc gcagtttaaa gtgacacaaa ttctgaaggt ttcatggttc tggatcgatc
    12121 tggcggtccc tgtcggcgca ctggcaacgg ctatttatac cctctactgg ttaataaacg
    12181 atacaaaaca atccgatagc ggagacaaaa tgcaatgagt tggttattga ttattccgct
    12241 aatgatcgtc tgtttgtttc tcaacattcg ggtctatctg gcgatgttta ttggcatcat
    12301 cgcttacttt ttattcttct ccagcgtacc gattgaaatt gccgtgcaaa gactaatcgc
    12361 gccaacgcaa agcccatcat tgctggcaat tccgtttttt atcttgctcg gcacactaat
    12421 gagctacacc ggcattgccg aacgcatcct gcacattgcc aacgttctgg tggggaaaat
    12481 gcgcggcgga ctgggcgtgg caaacattct cgtcagcacc atgatgggcg gcgtcagcgc
    12541 ctctaacctt gctgacgcgg cgatgctgtc gcgcatgatg gtgccggaga tggagcgtaa
    12601 aggctacaat cgcgcgtttg ccgccgccat taccgctggt ggctcgttag tcaccccgat
    12661 cataccgcct ggaatagccc tgattattta cggtctggtt gccgatgtct ctatcggaaa
    12721 aatgtttatc gccggactgg tacctggttt gctatgcgca gtcgtactga tgtttaccgt
    12781 ctaccttgtc gcccgcaaaa ctaacgctaa accctcgcgt gaaagctggc ctacgggcaa
    12841 agaggtggtg ttctcgctgg gacaggcctg gcatgcgctg ttcctcattt ttgctgtggt
    12901 gggtggtatt cgcgctaaca ttttcacccc aacagaagca ggtgcagtag cggtgctaat
    12961 cgtgctggcg attggctttt ttatctatcg tgaaatgcgg atttctcatg tcgtcaaagc
    13021 attgggcgaa acggcacgcg caaccgcatc ggtgatgctg gtaatcatgg ccagcgccgc
    13081 attgggatgg attttctcaa tggaacatgc tggtgtcgca gtggcgaatt ttgtgacttc
    13141 gctgaccgaa aacaaataca tgttcctgtt gattatcaac atattgctac tgacgctggg
    13201 gatgtttctg gaaggaaacg ccattttgct gatcctggta ccactattaa aaccggtggt
    13261 cgaacacttt ggcatcgatc cggtgcattt tggcatcatc atgatcttca acctgtcgat
    13321 tggcgcattc acgccaccgg taggtaccgt gatgctgctg gtatgtaata tcacgcaagt
    13381 ttcggtggga aatttcttta aacaatccct gcccctactg gcggctctac tgttaatgct
    13441 aatgctggtg acatatatcc cggcgatttc actgttcctg gtttaataat aaaaacgccg
    13501 gatgaaatac tcatccggca tcattacgtt aaaacgtttc ccagttctcc tcgctatctg
    13561 ccacggccat tttgcgcggc gtagctggcg tcacggtttt taccacagcc gatgcttcac
    13621 gctgctgttg ctgctgttgc tgaatccgga acactgccac tgcttcggtc aggcgactgg
    13681 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttttgtt
    13741 gggttacccg gtccatctca gcaaccgcta agccaacctg atcgatgcca cggctctgct
    13801 catcagaagc agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcactgacaa
    13861 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccagcatcc actttcccca
    13921 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg agcgctacgc tgggccagat
    13981 tacgcacttc tcccgcgacc accgcaaaac cacgtccttg ctcacccgca cgcgcagcct
    14041 caaccgccgc gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga
    14101 taatatcggc gattttctgc gaactggtgg agatatcgcg catggtctgc accacgttat
    14161 ccaccacttt gccgccgcgt tgcgccgttt cggaagcact taacgccaga tggctggcct
    14221 ggcgcgcatt ttcggcattc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    14281 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg
    14341 cgatttcgct ggcaccgcta taaatggcat tggccccgtt gcgcacatca ccgacggtac
    14401 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    14461 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    14521 ggcgattcat tggcgcgacc agcgaggctt taataccgaa ccagacggcg aggatgaccg
    14581 ccagtacgac gatcatcacg cctaccagaa tccacatcgc ctggctgtag gaggcattgt
    14641 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    14701 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    14761 cacctaacag ttgtatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    14821 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    14881 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    14941 aaccgctacc aatattgttc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    15001 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    15061 tttgtaaaac agtgaaattt tctttgtcgt tctttaaggc attaaagaac agaccgcctg
    15121 aagtcagttg taaaaggcca aaaacggcca aaaccagcag taggctggtc acaattttga
    15181 tacgttttaa catggtttct ctattcctgt ggaccgatgc aagattttcg gccaggaaag
    15241 gaaaaacttt atgaatatcc cgcggagatg acgccgagta attttattca ccccctgaat
    15301 ttaaaaagcc attatttaca taaa
//
LOCUS       DADTFG010000119        15194 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.120, whole genome shotgun sequence.
ACCESSION   DADTFG010000119 DADTFG010000000
VERSION     DADTFG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.120"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(77..898)
                     /gene="dapB"
                     /locus_tag="J1138_003436"
     CDS             complement(77..898)
                     /gene="dapB"
                     /locus_tag="J1138_003436"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBA8455196.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(1059..1424)
                     /locus_tag="J1138_003437"
     CDS             complement(1059..1424)
                     /locus_tag="J1138_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA8455197.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPVSFILTLIFAVPAIALAVRRMHDIGYSGWWVTIAVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1447..2361)
                     /gene="rihC"
                     /locus_tag="J1138_003438"
     CDS             complement(1447..2361)
                     /gene="rihC"
                     /locus_tag="J1138_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBA8455198.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNCQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYICRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2427..3377)
                     /gene="ispH"
                     /locus_tag="J1138_003439"
                     /gene_synonym="lytB"
     CDS             complement(2427..3377)
                     /gene="ispH"
                     /locus_tag="J1138_003439"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBA8455199.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDATDIQEEWVKEAKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3379..3828)
                     /gene="fkpB"
                     /locus_tag="J1138_003440"
     CDS             complement(3379..3828)
                     /gene="fkpB"
                     /locus_tag="J1138_003440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBA8455200.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3936..4430)
                     /gene="lspA"
                     /locus_tag="J1138_003441"
     CDS             complement(3936..4430)
                     /gene="lspA"
                     /locus_tag="J1138_003441"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HBA8455201.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4430..7246)
                     /gene="ileS"
                     /locus_tag="J1138_003442"
     CDS             complement(4430..7246)
                     /gene="ileS"
                     /locus_tag="J1138_003442"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBA8455202.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7289..8230)
                     /gene="ribF"
                     /locus_tag="J1138_003443"
     CDS             complement(7289..8230)
                     /gene="ribF"
                     /locus_tag="J1138_003443"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBA8455203.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHCALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8238..8456)
                     /locus_tag="J1138_003444"
     CDS             complement(8238..8456)
                     /locus_tag="J1138_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HBA8455204.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8559..8822
                     /gene="rpsT"
                     /locus_tag="J1138_003445"
     CDS             8559..8822
                     /gene="rpsT"
                     /locus_tag="J1138_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBA8455205.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(8918..9823)
                     /gene="nhaR"
                     /locus_tag="J1138_003446"
     CDS             complement(8918..9823)
                     /gene="nhaR"
                     /locus_tag="J1138_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBA8455206.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(9883..11049)
                     /gene="nhaA"
                     /locus_tag="J1138_003447"
     CDS             complement(9883..11049)
                     /gene="nhaA"
                     /locus_tag="J1138_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBA8455207.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLVVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            complement(11284..12543)
                     /locus_tag="J1138_003448"
     CDS             complement(11284..12543)
                     /locus_tag="J1138_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA8455208.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            complement(12672..14165)
                     /locus_tag="J1138_003449"
                     /pseudo
     CDS             complement(12672..14165)
                     /locus_tag="J1138_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14185..14946)
                     /locus_tag="J1138_003450"
     CDS             complement(14185..14946)
                     /locus_tag="J1138_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455209.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDYLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
BASE COUNT         3990 a         4014 c         3465 g         3725 t
ORIGIN      
        1 aagggccaaa aattaaagcc ctttatattc aatgaattaa atgtgttatt tttgcatcac
       61 cataaatatc ttgtggttac agattattga gatcaagcac atctcgcata tcaaaaagac
      121 cactttcctt accactcaac cacaaagccg atcttaccgc accgttagca aatgtcatgc
      181 ggctggatgc cttatgggtg atctccagac gctcgccaat atcggcaaac atcgcggtat
      241 gttcgccaac gatgtcacct gcacgcacgg tggcaaaacc aatggtgcca ggcacacgtt
      301 caccggtatg gccttcacga ctgtagaccg cgcaatcttt cagatcttta tcaagagcgt
      361 gggcgatcgc ctctcccatt gccagtgcgg tgcctgacgg cgcatcaact ttatgtctat
      421 gatgtgcttc aataatttcg atatcggtgt agtcccccat cactttggct gctttctcca
      481 gcagtttaag catgacgtta acgccaacgc taaaattggc ggcaaagaca atcgcaatat
      541 cggcggcggc gtcacgaatc gcctgtttac cggcttcgtc aaaccccgta gtgccgatca
      601 ccatcccttt gccatgctgg cgacaaaaag cgagatggtt cagcgtacct tccggacggg
      661 taaaatcgat aaacacatca aaatcatctt ttatcgcatc aagactactt tgcacagtaa
      721 cgcctgtttt cccagctccg gctagttcgc ccgcatcgct gcccagtaaa gaagatcctt
      781 cgcgctccag cgccgcgccc agctgcacgc cttctaatgc cagcgccgcc tgaatcaact
      841 ggcggcccat acggcccccg gctcccgcga tggcaacgcg gatgtttgca tcatgcatag
      901 ctattctctt ttgttaattt gcatagaccg ttttcagagt aaccagcgca tcgctacgcc
      961 gccagccaaa aacgctgata attagaaatt aatgataaac agggagggat atcagtgaaa
     1021 ggcatgacgc agaaagtgat gcgtcatacc ctctggcatt atgcctggtc ttgtgacttt
     1081 gacggcaggc agcacagtat aagcagaatt acgcccgtaa cagggatcaa cacggcaatg
     1141 gtaacccacc accctgaata tccgatatcg tgcatccttc tgacggctaa cgctatcgcc
     1201 ggtacggcaa aaatcaacgt taaaataaat gacacgggca gcgcaaaaaa aatgaaaaca
     1261 ccatagtcat tgttaattgt taccgctaac caccaacata aaagcggaag cagacagata
     1321 acgccactac caatcataaa cgaccagaac tcgaagcgat tggaaacacc ttcataatta
     1381 aagatgtttt tccacccttc cagatacgct tctccatacg tcatagaaca cttccttgtt
     1441 ttattattag agcgccaggg ccagcacctc agccacccac tgctggaagc ctttcacatc
     1501 cagatccagc gccacctgta cattggctgg tttgcccagg caaccgtcga tatcaacaac
     1561 cgtcgtgcct gaggtaaatt caccctgagt ttctaccgcg acaaaacacg gtttgagagt
     1621 gaacaattcc gggcgcacca gccaggcgat agcgcaaaga tcgtgcatac gtagtccact
     1681 ttgcatactg ccgctacggt agtggctaaa cagggcgtgt agcatttttc cggtgcgatt
     1741 caattccggc aaagtagcga gatagtcagg ggttaatatt gcctgattgg tgacatccag
     1801 accgcacatg acgatttcga tgccgctacg gaagacacag gctgccgctt ctggatcggc
     1861 agcgatatta aactcggcgt ttggtgtgca gttgccgcga ccagcagaac cgcccatgat
     1921 caccagacgg caaatatacg gcttacattc cgggcattgt gaaagtaaca gcgcaatatt
     1981 ggttaacggg ccgatggcta ccagggtaac gggctctggc gcacgcatca gggcatcacg
     2041 aattgccaga aacgccggta tacccagtgg ctgacagttg tgttcaacaa agtcatagcc
     2101 agccattccc gattcaccgt gtacagatgc cgcatcgcgc ggcgcacgaa ccagtggtac
     2161 ggcagcgccc tgggcaagcg gaatatccac attccagaaa tgcagcagtt gcagggcgtt
     2221 gcgggtggtt ttctctaccg aaacattacc cgcgacagtg gtcatcagtt gcagatcgag
     2281 ttcgggggca aaaatcgcgg cggcaatggc aacggcatcg tcaatgccgg ggtctgtatc
     2341 gaggaagata ggtaaacgca tgttttctcc ataaaaaatg ccggtaacaa gaccggcatt
     2401 ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac acgcagctct
     2461 ttcggcactt cgaaaacaat gttttcttca cgaccttcca gcggaatggc ttcgccaccg
     2521 ccaagctgct gtaaacgtgc gactacgttc tgtaccagaa tatccggagc tgatgcgcca
     2581 gcagtcacgc cgacgcattt cgcctctttc acccactctt cctggatatc tgtcgcatcg
     2641 tcaatcaaaa acgcgcgttt gcccatacgc tgggccagtt ccgccagacg gttggagttg
     2701 gaagagtttt tcgaaccaac caccagtact acttccgcct gctctgccag ggcgcgtacc
     2761 gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc gacaattttc
     2821 gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga cagcgtggtt
     2881 tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac atcgtctggc
     2941 gattcgacca gatacattcc cccttccggg ttgctgtact ggcccatcgt cccttccact
     3001 tccgggtgcc cggcgtgacc gatgagaata gattcttcac cacgacggct ggcgcgggcg
     3061 acttccatat gcactttggt caccagcgga caggtggcgt caaataccgt caaatcacgg
     3121 cttttcgctt cattacgtac cgcctgagaa acaccgtgtg cggagaaaat caggatcgcg
     3181 ccgtccggta cttcgctaat ctgctcaata aagatagccc cacgctcgcg caggctatcg
     3241 accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc gtaaatggcc
     3301 agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc acgcgggttg
     3361 gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca cttcaatatc
     3421 aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg agtcgccgtt
     3481 aatttcgcgg atcacgccag gcatctcact gccatccatt gcggtaaaaa gcatgattgc
     3541 gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact gaatcaggtc
     3601 cggtgacggc acgccaaacg ccgcatcagg ctccagcgag aaggtggttt tatcgcccac
     3661 tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcac ccaggcggaa
     3721 cagcgccggt ttaccgttgt tgcgggtaga ctcagcggtg gtgccatcat cgagtttcag
     3781 cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc aggtttgctc
     3841 ttatattttg aggatttttg ccggatgcgg cctacgattt cgcagcaatc tgtaggccgg
     3901 ataagatgcg tcagcatcgc atccggcagg gtttattatt gttttttcgc tttagaaggt
     3961 aaaaaacctt ccagcacaat cagtgccgca ccgacacaaa tggcagtatc ggcaaggttg
     4021 aaggtggcga agtgccagtc gccgacatag aagtcgatca tatcgacaac gaagccgtgc
     4081 cacaggcggt cgaacaggtt gcccagtgcg ccgccaataa tcagcgcgta agcgatattg
     4141 tttagcttct gcgtggcctt cgagcgatac atcatcactg ccaggatcac gctaatacca
     4201 atcgcaatac cggcaaagaa ccaacgctgc cagccgccgc tatcggcaag gaaactaaac
     4261 gctgcgccat agttacgcgc ataatgcaga ttaagcgacg ggaacagcgg gaccgtatcc
     4321 cccagagcaa agttctggag gatcaggtat ttgctgccca gatcgataat cagcacgact
     4381 accaccagcc acagccagcg tagccctgtt gaacagatcg attgactcat caggcaaact
     4441 tacgtttttc accgtcaccg gcgacgttgc tgacacagcg gccgcagatt tctgcgtgtt
     4501 ccgccacctt gccgacatcc tgggtgtagt gccagcagcg agggcacttc tcaccttcgg
     4561 ctttactcaa cgcgactttc agccctttga ggacttcgct ctgctgggcg tcagcaggtg
     4621 cgtcgttata gtctgcaacg gtagcgccgg aggtcaacag gacaaatcgt aattcatcgc
     4681 ccagcgcggt cagtttcgcc gccagttccg gttctgcata caaggttact gccgcttcca
     4741 gcgagccgcc cactttctta tcggcacgcg cttgctcaat gactttgttc acttcgccac
     4801 gcactttcaa cagctcgtcc cagaacgcat cgttcattgc ttcactgtct gccagaccaa
     4861 acaggccttc gtaccactcg ccggtgaaga cgtatttttc acgttcgccc ggcaggtaac
     4921 cccacacttc atcagcggtg aaggagagga ttggtgccat ccagcgaacc agcgcttctg
     4981 cgatgtgata cagcgctgtc tggcagctac gacgcgccac gctgtccgct ttggcggtat
     5041 actgacggtc tttgatgatg tcgaggtaga aggaacccat ctcaacggag cagaagcgca
     5101 tcagacgctg taccacttcg tggaaatcgt atgcttcgta cgccttgagg atgtcttcct
     5161 gtgccgcttt cgcacaacct acggcccagc gatccagtac caccatctct tccggtttca
     5221 ccatatcttt tgccggatca aaaccgttca ggtttgccag caggaagcgc gcggtgttac
     5281 ggatacgacg atagctgtcg gcagcacgtt tcaggatctc gtcagaaacg gccatttcgc
     5341 cagtgtagtc ggttgatgcc acccacagac gcagaatatc cgcacccagt ttgttcatca
     5401 catcctgcgg cgaaacggtg ttaccgatgg atttagacat cttgcgaccc tgaccatcca
     5461 cggtaaagcc gtgagtcagt acctgacgat atggcgcttt gcccttcatc gcggtagaga
     5521 tcatcaggga agacatgaac cagccacggt gttggtcaga accttccaga tacatgtccg
     5581 ctgcgtgacc ggcaaattcc ggacgcacat caacaacgga agagtgggta gatccggagt
     5641 caaaccatac atccagcgta tccggtactt tcacatactg gtcagcttcg tcgccgagga
     5701 tctctttcgc atcgagatcc caccacgcct ggatgccgtc aacttcaacg cgttttgcca
     5761 cttcttccat cagttcgaga gtacgcggat gcagttcttc tgtgtctttg tgcacgaaca
     5821 gtgacatcgg cacgccccag gtacgctgac gagagataca ccagtcagga cggttagcaa
     5881 ccatcgactc gatacgcgcc tggccccagt cagggatcca ctgcacgcct ttgatctctt
     5941 tcagtgactg cgcacgcaga cctttctgat ccatgctgac gaaccactgc ggcgtcgcgc
     6001 ggaagatgat cggcgtttta tgacgccagc agcacggata gctgtgttgc attttctcaa
     6061 cgtgcaacag tgcgcctttt tcctgcaaca acgcaatgac aatatcgttc gctttgaaga
     6121 cgttaacgcc atccagagtc gggtaagtac ccggcagata agtgccgtcc gggccaaccg
     6181 ggttagcggt ttccagacca tatttttgac cgatcacata gtcgtccgga ccgtggcctg
     6241 gcgcggtatg aacggcaccg gtaccggcat ccagcgtaac gtggtcgccg agaattgccg
     6301 gaacatcgaa gtccataaac ggatgggtaa agcgcaacag ttccagctcg gcacctttca
     6361 ccgtgccaag aatggtgtaa tcgctaacgc cgatacgctg cattacgctt tcaaccagat
     6421 ctttcgcgag gatcacggcc tgaccgtcga tttgcaccag cgcataatca aaatcaggtg
     6481 caatggagat tgcgcggtta gcaggcagcg tccacggcgt ggtggtccag ataaccagcg
     6541 aaattgggcc gttaacatta cttacgccaa atttcgtttt cagcgcatcc tgatcgaccg
     6601 cctggaaagc gacgtcgatg gacggagaag ttttgtcgta atactcaact tccgcttctg
     6661 ccagtgcaga acggcagtca acgcaccagt gcaccggctt cgcgcctttg tgcaggtgac
     6721 cgttgccgat gattttgccc agcgcgcgga tgatgttggc ttcagttttg aagtccatgg
     6781 tcaggtacgg gtgcgaccag tcgcccagca cgcccagacg gataaagtct ttgcgctgac
     6841 cgtcaacctg ggtcgcagcg tattcgcggc acttggcgcg gaactcagcg gcggtgaatt
     6901 tctcccccgg cttaccgtat tcttgctcta ctttcagttc gattggcaga ccatgacagt
     6961 cccagccagg cacatacggc gagtcatatc cagaaagccc tttggactta atgataatgt
     7021 ctttcagaat cttgttaacc gagtgaccaa tatgaatgct gccattcgca taaggagggc
     7081 catcatgcag aatgaaggtt tttttgcctt ttttagctgc acgaatgatg ccgtacagat
     7141 catcatcagt ccaacgcgcc agcattcccg gttcgcgctt ggcgagatcg ccacgcatcg
     7201 ggaaccctgt ttccggcaaa ttcagggttg atttatagtc actcatcaga ttctcggttc
     7261 cgtatttcgg tttgattaca taacaggctt aagccggttt tgttagccca aaaaattcgc
     7321 gggcggttaa ttcatcacgc gcaatctgcg ctttcagttc gtccagcgat gcaaatcgct
     7381 gctcattgcg tattttttta cgcagcacta cttgtatatg gcgaccataa aggtccattg
     7441 caacatctaa caaatgcact tccagttgct ggcgaatacc ggcaaccgtt gggcgtgttc
     7501 cgatgtttgc aacgccgggt aacggctttt cgccaaggcc caacacttct accgcataaa
     7561 cccctttcac cggggaaacc tgacggcgta gcggtacatt cgccgtcggg aaacctatag
     7621 tgcgccctaa ttcatcaccg tggactacac gcccggagat agcaaacggg tgccccagta
     7681 aactttctgc cagagccaga ttgtcatccg caagcgcctg acgcacggcg gtgctgctga
     7741 tacgcacgcc accttcgcaa aaagtttgcg tgctggtgat atcgaagccg tattccatgc
     7801 ccgctttctg taataacaag aaatcgcctt cacgaccagc gccaaagcgg aaatcgtcac
     7861 ctacggcaag aaattttacc cgcaagtgct tcaccagaag atcactgatg aagttttgcg
     7921 cggttaacgc cgcaaaacgc ctgtcaaaac gcacgcacag cacgtaatca acaccacact
     7981 ctgcaagata acgcagtttt tcccgcagcc gggtgagccg tgccggggct ttatcagtag
     8041 caaacagttc cagtggttga ggttcaaaaa gcatcaccat caccggtaag ttgcgcttgc
     8101 gcccttcttc ctgcaagccc tgtaacagcg cgcaatgacc gcgatgcacg ccgtcgaaat
     8161 taccaatagt cagcacacac ccttcttgcg gggcctggct gagattatgt atgccgcgta
     8221 tcagcttcat gtctggctca aaacagtgaa aatcgtccga gtataccttg tacagcggtc
     8281 aaggttaacc ggcgattgag taccgaaatc agaaagaagt ggcttttcat tgctatggcg
     8341 caaatcatgg gaagaaactg accgcctgct gcaatttttc tcgcggaaaa gctgtattca
     8401 caccccgcaa gctggtagaa tcctgcgcca tcactacgta acgagtgccg gcacattaac
     8461 ggcgcttatt tgcacaaatc cattgacaaa agaaggctaa aagggcatat tcctcggcct
     8521 ttgaattgtc catatagaac acatttggga gttggacctt ggctaatatc aaatcagcta
     8581 agaagcgcgc cattcagtct gaaaaggctc gtaagcacaa cgcaagccgt cgctctatga
     8641 tgcgtacttt catcaagaaa gtatacgcag ctatcgaagc tggcgacaaa gctgctgcac
     8701 agaaagcatt taacgaaatg caaccaatcg tggaccgtca ggctgctaaa ggtctgatcc
     8761 acaaaaacaa agctgcacgt cataaggcta acctgactgc acagatcaac aaactggctt
     8821 aattgcctgt tgttgtcagc tttgtaaaaa aacccgctct ggcgggtttt tttatatcca
     8881 tctgcaggcc aaaattcggc ctgcaaagta caaatgttta ttttgaagct ggagtaaaca
     8941 gcgcagaata gtctgtattg cagatacgct gtactgcagg gtgctgaatc atcctttcgg
     9001 caaaaatcgc gtggtactct tccatcacgt tctcaacacg gcctatctcc acaaccgagt
     9061 catcgttata gaaatcatta gcgtaaagcg aaggtgcaac gaaaatagcg ttatgcgtcg
     9121 ccccaaaggc tttcatcaac gcagcatcat caaactcacc caaaatttcg acgttcaagc
     9181 cctgggagtt aaaccagttt aatagtttac gccccaacat tgagcgacgc cccggaataa
     9241 gtaaacgacg ctcttcaaga caggcaggaa acggcttttc tggtagtggg ttagtgcacc
     9301 agaaactgac accacattcg ccaattttca tggaaaacag cccttcctgc tgagtggaat
     9361 cgatcggaca gtcagagatg atcatatcca gtttatgctg actcaactgc tctaaaagca
     9421 tctcgtgcgt cgattcgaaa cagcgtagat ggatctgctc gtcttccaca actgcggcat
     9481 ccagaacact gctgaccaga cgtttggaaa gtgcatctgc cacaccaaca tcaaacaata
     9541 agttggactc tttgcgatag ttgacgatat ccagcatttc ctggcttaag gtgaacattt
     9601 tatcggcata gcgatagacc agttccccca gttcgctggg ttccagacca cgtcctttac
     9661 gcttaaatag tttcccttgc aggcgctctt ccagcgcccg gatctgcccg gtaatggttt
     9721 gtggtgttaa ataaagcgcc tccgctgcgc caaccacaga accttctttg tagacatgcc
     9781 agaagtaata caagtggttg taattgatat gagacatgct catttctctc cctgataaca
     9841 atgaaaaggg agccgtttat ggctccccag tacatcgtcc tgtcaaactg atggacgtaa
     9901 acgaacgcgt aaccagctat atccaattac cgccgaagaa attgaaccga caaggatacc
     9961 taattttgcc cagttaatca gttctggatc tacgctacca aatgccaggc tggcaataaa
    10021 gatagacata gtaaaaccga taccgcacag gataccaacc gccataattt gctggtaagt
    10081 cgttccctct ggcagatgtg ccaatttcaa acgcagcgcc aaccagcaga acagactaat
    10141 acccagtggc ttgccaatca gcaaaccagc gatgatccct aatggcagga tggaggtcaa
    10201 accttccagc gtgacacctt gcagtgaaac gccagcatta gcaaatgcaa acagcggcaa
    10261 aatcagatac gccacccatg gatgcaaaac atgctccaga cgtttagccg gagagcgccc
    10321 atgcttctct ttcaaaggaa taaagaagcc gacaatgacg ccagccaggg ttgcgtgaac
    10381 ccccgatttc aacaccgctg tccacagcac cacgccaacc agaatataaa cgcccgtgcg
    10441 gcgtacacca cacagattca ataccacgag taccgcaatt gctacagccg cgacgccaag
    10501 agaggccatc gataagtcat tagtgtagaa caatgcgatg ataatgatgg ccccaagatc
    10561 gtcgataata gccagagcca tcaaaaagat cttcagcgct aacggaacac gacttcccaa
    10621 cagcgccaac acaccaagtg caaaggcaat gtcagtcgcc gccgggattg cccagccttc
    10681 gcgggtaatc ggatcggcat agttaaaagc cagatagagc aatgccggga caatcatccc
    10741 gccgattgcg gcaataacag gaaatgccgc ctggcgcaga ctggccagcg aaccttgcat
    10801 cagctcgcgt ttaacttcca gaccaaccaa caggaaaaat accgccatca gagcgtcatt
    10861 gatccatagc agcatgttct tgttgatctc aagtgtccca acccggagct gaaccggcgt
    10921 ctcaagaaag tcgtgatacc atccactggt tgcaccgctg ttggccataa tcatcgctaa
    10981 tacagcggca ataatgagaa taatgcctcc cgaggcatca ctgctaaaga atcgatgcag
    11041 atgtttcact ttttatttct cttcaggtga atagatcgtt ttcacgattt taccccgcac
    11101 gaatcatcgt taaaatcaga ttatagtgta ggaatagctc gctttttacg atctattaaa
    11161 agaattgcgc catcagcgag agatgagttt ttgatgcccg aaagcggcgg ctgatgacct
    11221 taaaccccat taatcatagt ctaatgtcaa gatttctaaa gaaattattc ggaaaagtaa
    11281 taattaaatt gttccatgct ctagtttttt attccacaga tgtttgagga tatctaatgt
    11341 agaaacgaca ttcgcaggag acattcggtt caatgatata aggaaaggat ggttctccac
    11401 aaagtggata aaacgcgtcg cactgttcaa tgttgcatgc cgcatctcgc ggactttctt
    11461 actgccagtg agattgcata cagccattga ataattgcgg ttagaaaatg cacgccagac
    11521 attatttcgt accccaccac taaggaataa tcgcggtgga aaatcaatgg tggcgggtgc
    11581 cacaccgcgc tctttgacaa aggaaatatg gcagcgacct tgatgaatat gctgcaaatt
    11641 agccatcagg cagccgccca atataaagca ttcgtgatca aggtaatgct gaacgttgtt
    11701 ggaatgctca tcggtcagta ttctgaccag ttcaaaatga cgatatgccg gaagaaccgg
    11761 ttttaccaac attcccggat cgttttgatg taaaactgcc gggccgtaat caatatcaaa
    11821 caaggtatcc cgccgcatag taagtaactc tttgcgctgg acatgatgtg ccagcgtccc
    11881 tggagacata cgctcttcat agttcgatgt gtattgataa tgttgctcta cggcagatgg
    11941 ggagtaattg gttgagatag caaccaccct gccgctttgt tcttctgcgg taacaagagc
    12001 ataaagagag ttattgctac cattatactt gacgcgaaaa gcgcgggtac tcagggtaat
    12061 atcagaagct gggaaacact gtcgactttc tttatagttt gcttcacgcg ctaattttaa
    12121 cagttcacgg ctaagtccag tgctgttaag aagtaatgac tttctgatat ccgcaagtgc
    12181 ctccccttgc tcaatcatca atgctaactg ggctcctcgt ggtgtggtaa ttacgtgttc
    12241 cagagtgata aatgttttct cacaatgatg gcaatacatt cttctctggc cttgtgcaga
    12301 atatccatat tttttgaggg acgtgcctcc gcaagttgaa cattggcaaa tcaaacctct
    12361 ccaggagtga ttcacaatat tacgataaat attaagcgac tgttcagata tcactggaaa
    12421 caagtaacca cattcacggc ataaaatatt tttaccctgt gcgacataat cctggctatt
    12481 cagcaacccc aagtttttac atcctggtgt tttgcaacaa tcaacattta cgttggtaaa
    12541 catatccact ccctgcgaat agccgtatct tgatattgtt caacgcgaaa agaacctgat
    12601 tcaatgcgta gcactgaatc aggcgggcat agccctaaat ggcatacatt acatttatta
    12661 tgattatcag gttagcccct ggtcaccgct tctggtttat cacctctggc tttcattaga
    12721 gaatcattat caatatcagt tttatatttt aaaaacttac catacatctg tttttcaata
    12781 tctggttttt taccgatcag attaagtgtt tcataacgat cagatttcag attgtagaga
    12841 tatttcggtt tattattgcg atcgataatc atcttccagt caccatcacg gatcgcccat
    12901 tcatcggttg gatcatcctg gaatggcata tcaatcccga aaattaatgg cttttcgcgt
    12961 ttcaatgctt tttgctcaag aacaggaacc agcgattcac catcgaaagt acggtctgta
    13021 ggtaatttga agttcatcat tttcgctaaa gtaggcatcc agtccagacc ataaacgggt
    13081 gtatctgaaa ccattccctg tggtagatgt ttaccatatt taataatggc tggaacacga
    13141 attccgcctt cccaaaggtt atccttgcga ccgcgtaatc catccgtttc ccctgccaaa
    13201 ttcagctcat acactttgcg cgcttcacgc gttaccggac cgttatcact ggtaaaaata
    13261 acgattgtgt tatcttcttc acccatcgct ttgattttat ccagcacttt tccaacctgt
    13321 gcatccagat agctgatatt ggcataatat tcccccacac cacgccaggg tttgtctgcc
    13381 cagtcgccat aaaataaatc aggatgctgc ttctgatacg cgctcatata ttgtgagtac
    13441 atgtcgaggt attttttggg cgaagccagg gggctatgca cttcggtaaa agcaacatag
    13501 aggaagaaag gcttgctgtc ctttttgtta tccagccagt tgacgacttc cgaactgaca
    13561 tactcaccgc tcattttatc ggctcgtgga gtgggttgcc cattacgtag ccagcctgtc
    13621 gggtaaacca tgccataacg cgggcgttct ttagcgttat ccagcgtggc gtcggtaaca
    13681 aagcccgccg tattagccag tgagtaatca aagcccatat cttgtgcttg tggctgatcg
    13741 gtgcgatcgc cgcctgcatt cagatgcagc ttacccatca ttgccgtgtc gtacccttgc
    13801 gctttgagta gattagcaat cgtgagttcg ttacgcccta aggcaacatc tttgcctgaa
    13861 ggaatccatg agcgaattcc agtacgaaat ggcatccggc cggttaatag ccctgcgcgt
    13921 gaaggtgaac ttaaaggagc gggggcatag tagtcagtaa atttgacccc ttcctaggca
    13981 agcctgtcga tattaggtgt tttaacgatc tgatgaccat atgttgctaa atcgccataa
    14041 cctaaatcat ccgccataat aatgactaaa ttaggttgtt tagcctctgc ggctaaggca
    14101 ggactaaaag cattccctgt gcaaactgca aggccgatca aactggccat taacgttttc
    14161 tgcataatta ttccctgata aaaattagaa atcataacca agcatataaa tcatttgatc
    14221 gccaaagcca tcgtagccca gcttattatc gaaataacgc cacgtcacac ttgctttcca
    14281 gcgaggatag aacttccagg caatagttaa accgccattt aacccgttcc ggccaaattg
    14341 ctgctccgtg taagcatcgt tcctgtccag ttctatttca ttccagttag ataaaacaaa
    14401 tttttcgtca aataacgtaa atggtaatac tgctgtccag ccaacaacat aaccattcca
    14461 accattcgtt tgaccatatt tagctgatac gtagtcacca gattgattat gcaatccaat
    14521 atacggttta aaaaagcccc actcgccgtt ccagccgagg taaccgaatc cataaaacat
    14581 gtccagatag tcaccccatg agttatccca ttggccataa attttgccaa agaatgtcat
    14641 atcagagtca aagagtctga catgcgtcat tgctgaaacc gtatgatttc tccctgctac
    14701 agcatgattg agtacattac tttcataaaa tgagtaaaat tctgcttctt taaaatttac
    14761 cccaaaatcc gcagtcactt tccatacttc accacggtga gtgtttacaa atccactatt
    14821 ccagtctgca taacccgcat ttattgaacc aaaggttcct ttatattcta atgaagaata
    14881 tgcaggaaaa tgatagatag ctgttatcaa ggtacacgca ataagaagtc gtcttttggc
    14941 agacatataa tatgttctcc attcattgtt tttatgattc cataggaatt aatacaccag
    15001 acaattcatt tttattaaga atgaccaggc cagtatattt aaagatgact atcgaagcgt
    15061 gatgaagatc gcagattact tttgcgaacg ggcggaggga tatcgtgttt cagaaaatgt
    15121 gtctgtttta aacatcaccc tttgatttat aggcattttt catgatgaac ttagcattag
    15181 cggctgaacg tatt
//
LOCUS       DADTFG010000120        14999 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.121, whole genome shotgun sequence.
ACCESSION   DADTFG010000120 DADTFG010000000
VERSION     DADTFG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.121"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(23..2302)
                     /gene="maeB"
                     /locus_tag="J1138_003451"
     CDS             complement(23..2302)
                     /gene="maeB"
                     /locus_tag="J1138_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBA8455210.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2591..3541
                     /gene="tal"
                     /locus_tag="J1138_003452"
     CDS             2591..3541
                     /gene="tal"
                     /locus_tag="J1138_003452"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBA8455211.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMAPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3561..5564
                     /gene="tkt"
                     /locus_tag="J1138_003453"
     CDS             3561..5564
                     /gene="tkt"
                     /locus_tag="J1138_003453"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA8455212.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5667..6710)
                     /locus_tag="J1138_003454"
     CDS             complement(5667..6710)
                     /locus_tag="J1138_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBA8455213.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6836..7411)
                     /gene="nudK"
                     /locus_tag="J1138_003455"
     CDS             complement(6836..7411)
                     /gene="nudK"
                     /locus_tag="J1138_003455"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBA8455214.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATLLLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7479..9458)
                     /gene="aegA"
                     /locus_tag="J1138_003456"
     CDS             complement(7479..9458)
                     /gene="aegA"
                     /locus_tag="J1138_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBA8455215.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEQQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9664..11364
                     /gene="narQ"
                     /locus_tag="J1138_003457"
     CDS             9664..11364
                     /gene="narQ"
                     /locus_tag="J1138_003457"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBA8455216.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNHRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11528..14641
                     /gene="acrD"
                     /locus_tag="J1138_003458"
     CDS             11528..14641
                     /gene="acrD"
                     /locus_tag="J1138_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBA8455217.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNSERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14740..14799)
                     /gene="ypfM"
                     /locus_tag="J1138_003459"
     CDS             complement(14740..14799)
                     /gene="ypfM"
                     /locus_tag="J1138_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBA8455218.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3496 a         3986 c         3930 g         3587 t
ORIGIN      
        1 aagcgtgaga gttaaaaaag aattacagcg gttgggtttg cgcttcaacc acggccagcg
       61 ccaccatgtt gacgatacga cgtaccgatg cgatcggcgt taacacgtga accggtttcg
      121 ccacgcccat cagcaccgga ccgacagtca caccttccga gctggaaacg cgcagtaagt
      181 tgtaactaat gcgggcagct tccatgttcg gcatcaccag aatattagcg gaacctttca
      241 aagggctgtc cggcatacgg tcgttgcgaa tcgcttccac cagcgctgca tcgccgtgca
      301 tttcaccatc aatcatcagt tctggcgcac gttccctgac cagttccagc gcctgacgca
      361 ttttgctcga cgacgggcag tcagaagaac caaagttgga gtgcgacaac agagccacac
      421 gcggctcaat accaaaacga cgaacagttt ctgccgccat caaggtgatc tccgccagct
      481 cttctgcatc aggctcatca ttgacgtagg tatcggcaat aaaggtatta ccactcggca
      541 acagcagcgc gttcattgca cctgcggtgt gaacgccatc gcgataacca aagacatttt
      601 tcaccacgct aaagtgttca tgataatcgc ccaccgtacc gcaaatcatt gcatcggctt
      661 cgccacgctg aaccatgatc gcgccgatca ctgtcgggtt actgatcagc gcacgctgcg
      721 cctgttcctg agtgacaccg cgacgcttca tgatctggaa gtattcggtc cagtactctt
      781 taaagcgcgg atcggattcg ttattgacga tctcaaaatc aacgcccgcc ttgatctgca
      841 agcccagttt ctgaatgcgc atttcgatca cgttcggacg accaataagg atcggtttcg
      901 ccagtcccag cgttaccagt tcctgagtgg catgcagaac gcgcgcctct tccccttccg
      961 gcagaactac gcgcttcggc gctttgcgag cctgggagaa aatcggcttc ataaacaggt
     1021 tagttttgta aacgaactct gtcagcttgt cgatgtagac gtcgaaatca gcaatcggac
     1081 gcgtcgccac gcccgactcc atcgccgctt tcgccaccgc aggagcgatc ttaacgatca
     1141 agcgcggatc aaacggtttt ggaatgatgt attccggacc aaagctcaga tcctgatcgc
     1201 catacgctga agccaccact tcactctgtt ccgcatgggc cagttctgca atcgcgcgta
     1261 ccgccgccag tttcatctct tcgttgatgg cggttgcgcc aacgtccagc gcgccacgga
     1321 agataaacgg gaagcaaagg acgttgttca cctggttcgg gtagtcggaa cggccggtac
     1381 agatgatggc atccggacgc acttctttcg ccagcggcgg cagaatttcc ggttccgggt
     1441 tcgccagcgc caggatcatt ggcgcacggg ccattttctt caccatttcc tgggtcagca
     1501 ctttcgggcc ggaacagccg aggaaaatat ccgcgccttc aatcacatca tcgagggtac
     1561 gtttaccgtc atccaccacc gcgtatgcgg ctttggtttc cgccatgttt ggctcacggc
     1621 cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc agacccagcg
     1681 ctaccagcag gttcatacag gcgattgctg cggcacccgc gccagaaacc accatccgca
     1741 cgtcggagat gtttttctcc accacgcgca agccgttgag gatggcggca gtgctgataa
     1801 ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt catccgctcg cgcagtttct
     1861 gttcaatata gaaacattct ggcgctttaa tgtcttcgag gttgatgccg ccgaaggtcg
     1921 gttcgagcgc agcgacgact tcaataaatt tgtccgggtc gagttcatca acttcaatgt
     1981 caaatacatc aatcccggcg aatttcttaa acagaacgcc cttgccttcc atcaccggtt
     2041 tgcctgccag cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta gagatcaccg
     2101 ctaccaggtt accgcgcgcg gtatatttgt aggcttttaa cgggtctttt tcgatttcaa
     2161 gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc gttgccagag
     2221 gcttggttgg agaaacctgg attttccctg gaactggaaa ttcatggaaa tcaagtgcac
     2281 tttgttttaa ctggtcatcc atttggttgt tcctttcacg taacgttcac aaataaagtg
     2341 tgtgggcaac agcccctgtc cacaacgtgg cgcacattat taccctgccg gagtctacag
     2401 actttgagca agtccaaact ctcaccatta atataatgtt ttggtaataa tcctataaca
     2461 ctgatgttac ctgcttaatc cagcaatacc atgcctgtct gctatgcttt tttgatgcgt
     2521 ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc taacacttta cttttcaagg
     2581 agtatttcct atgaacgagt tagacggcat caaacagttc accactgtcg tggcagacag
     2641 cggcgatatt gagtccattc gccattatca tccccaggat gccaccacca atccttcgct
     2701 gttactcaag gctgccggat tatcacaata tgagcattta atagacgatg ctatcgcctg
     2761 gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg acaaactggc
     2821 ggtcaatttc ggtgctgaaa ttctcaaaat cgtacccggt cgcgtgtcaa cagaagttga
     2881 tgcacgcctc tcttttgata aagaaaagag tattgagaag gcgcgccatc tggtggactt
     2941 gtatcagcaa caaggcgttg agaaatcacg cattctgatc aagctggctt cgacctggga
     3001 aggaattcgc gcggcagaag agctggaaaa agaaggtatt aactgcaacc tgacgctgct
     3061 gttttctttt gcacaggcgc gggcctgtgc agaagcaggc gtttttctga tttcgccgtt
     3121 tgtcgggcgt atttatgact ggtatcaggc acgcaagccg atggccccgt atgtggtgga
     3181 agaagatcca ggcgttaaat cagtacgcaa tatctacgac tactataagc aacaccacta
     3241 tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg ccttaaccgg
     3301 ctgcgaccga ctgactatcg caccgaattt gctgaaggag ctacaggaaa aagtttcgcc
     3361 agtggtacgt aaattaatcc caccttctca gacgttccca cgcccagctc ccatgagcga
     3421 agcggagttc cgttgggagc acaatcagga tgcgatggcg gtagaaaaac tgtctgaagg
     3481 cattcgtctg ttcgccgttg atcaacgcaa actggaagat cttcttgccg ccaaactata
     3541 aaccagccac ggagtgttat atgtcccgaa aagaccttgc caatgcgatt cgcgcactca
     3601 gtatggatgc ggtacaaaaa gccaattctg gtcatcccgg cgcgccgatg ggcatggctg
     3661 atattgccga agtgctgtgg aacgattttc ttaaacataa ccccaccgac ccaacctggt
     3721 atgaccgtga ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc tacagtttgc
     3781 tgcatctgac cggttacgac ctgccactgg aagaactgaa gaacttccgt cagttgcatt
     3841 cgaaaacccc tggtcacccg gaaatcggct atacgccagg tgttgaaacc accaccggtc
     3901 ctcttggaca aggtttggcg aacgccgtcg ggctggcgat agcagagcgt acgctggcgg
     3961 cgcagtttaa tcagccggat catgaaattg tcgatcactt cacctatgtg tttatgggcg
     4021 acggctgcct gatggaaggt atctctcacg aagtctgttc gctggcgggc acgctggggc
     4081 tgggcaagct gattggtttt tacgatcaca acggtatttc catcgacggt gaaaccgaag
     4141 gctggtttac cgacgatacc gcaaaacgat ttgaagccta tcactggcat gtgatccatg
     4201 aaatcgacgg tcacgatcca caggcggtga aggaagcgat ccttgaagcg caaagcgtga
     4261 aagataagcc gtcgctgatt atctgccgta cggtgattgg ctttggttcg ccgaataaag
     4321 caggtaagga agaggcgcac ggcgcaccac tgggggaaga agaagtggcg ctggcacggc
     4381 aaaaactggg ctggcaccat ccgccatttg agatccctaa agagatttat cacgcctggg
     4441 atgcccgcga aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt gccgcctata
     4501 aaaaggctca tccgcaactg gcagaagagt ttacccgtcg gatgagcggc ggtttgccga
     4561 aggactggga gaaaacgact cagaaatata tcaatgagtt gcaggcgaat ccggcgaaaa
     4621 tcgctacccg taaggcttcg caaaatacgc ttaacgctta cgggccgatg ctgcctgagt
     4681 tgctcggcgg ttcggcggat ctggctccca gcaacctgac catctggaaa ggttctgtgt
     4741 cgctgaagga agatccggcg ggcaactaca ttcactacgg ggtgcgtgaa tttggtatga
     4801 ccgctatcgc caacggcatc gcgcaccacg gcggctttgt gccgtatacc gcgacgttcc
     4861 tgatgtttgt tgaatacgcc cgtaacgccg cgcggatggc ggcactgatg aaagcgcggc
     4921 agattatggt ttatacccac gactcaattg gtctgggcga agatggtccg acgcaccagg
     4981 ctgttgagca actggctagt ctgcgcttaa cgccaaattt cagcacctgg cgaccgtgcg
     5041 atcaggtgga agcggcggtt ggctggaagc tggcggttga gcgccacaac ggaccgacag
     5101 cactgatcct ctcaaggcag aatctggcac aggtagaacg aacaccggat caggttaaag
     5161 agattgctcg tggcggttat gtgctgaaag acagcggcgg taagccagat attattctga
     5221 ttgccaccgg ttcagagatg gaaatcaccc tgcaagcggc agagaaatta gcgggagaag
     5281 gtcgcaatgt tcgcgtggtt tccctgccct cgaccgatat tttcgatgcc caggatgagg
     5341 aatatcgcga gtcggtactg ccttcaaatg tttctgcccg cgtggcagta gaagcgggta
     5401 ttgccgatta ctggtacaaa tatgttggtc tgaaaggggc aattgtcggg atgacaggtt
     5461 atggggaatc tgctccggcg gataagctgt tcccgttctt tggctttacc gccgagaata
     5521 ttgtggcaaa agcgcataag gtactgggag tgaaaggtgc ctgatggtga tcgccggatg
     5581 ctgtatgttc ccctcaccct aaccctctcc ccagaggggc gaggggacgg tccgtgcgct
     5641 ttttaggggc gcatctggga ttttgtttaa cgggtgatcc acaacgtggg ccaggcgtct
     5701 ggcccatgcc agttatcgca ggtgggttct gcggcgtaac gcaccaggcg aaaacgctga
     5761 ccgtcaaagc gccagcgcgc ctgaatgcca caatcgctta atccgcgccc ttttgctaag
     5821 gtcaccagtt cacgcgattt ttcatcgaat gttgcgttca tcagttccag ttcattggtc
     5881 tcctgaccgt tgttgaacgg caaacgcaac cgtaccgggc gcgaggccag cggcttttta
     5941 cgcgacacaa tccatgccaa atcaatggtg ttataggccc cagcctcaca gctaatcatc
     6001 atcagcgctt tatcatcggt cagcgcagtg acattcacct cacgacgtaa cggatcaagc
     6061 gagcagcgca gaccattcat ccgccagttt ccataatcca gcaaatcgtt acgttcttcg
     6121 agtgagagtg gcgtcggcgt tgggttaacc accgcgacct ctttcagcgc aggcgctggc
     6181 ggtacgctga gcggcggttc gtcccctttc tttatccacg cggtctcact gccaacgcgc
     6241 ttttgctggg catcaataaa caacaacgcc gcttttaagc cactcagaga aatagtctga
     6301 ttgccgtcgc gtaaggtgat cgccctccct tcctgaatca tctgcaaaaa cgcggtgatg
     6361 gttgccgtat catcggttac taataaccat ggtgaaatcc gccacttgtc accacttaac
     6421 gccaacggct caccatctaa cagcagccgt ggcgctatct ccccttctga cgcatccggc
     6481 gacttcaacc cgccgcgctc aatacgtaaa acggcatcgg tatgcgcccc ggcgctgcgg
     6541 ctcagggtca tcaccagtcc attatgatcg cccgtattgc gcgctacaca gaaattttga
     6601 ttattacagg tgacctgcca gtcggaaaac gcccgttgcg ctggtgccgc ccacaccaaa
     6661 gacatcggca acaaagcgaa aaaaaagaga aggaaaatgc gatatcgcat ggacggtacg
     6721 accccagaag ttcaaacaaa cagcacaagt cgtatcttcg ttgcgcaggt agcgcggctc
     6781 aatcggattt gtcggattga gccaataata aacaacgaaa tattattgtt atttttcagt
     6841 ccattaaatg tgacatttgc aaatagttaa gcaataacac cgtcttaccg tcacgtatct
     6901 cgccggtttt aatcatctcc agcgcctggc tgaacggcag ctcaagcact tcaatatctt
     6961 catcttcgac accaccaccg gcgttagcgc gctggctgtc actgtattcg gcgataaaaa
     7021 agtggattag ctcagtcaca ccgcctggcg acatatacag ttcaaataat ttgcggactt
     7081 cgccaacttc atagcccgtc tcttcaatcg cttctttgcg aatgcacact tccggttcgt
     7141 cgttatccag cagcccggcg caggtttcaa tcagttgccc gctttcattg ccattaaccc
     7201 aggtagcgac gcggaactgg cgaatcagaa ccacggtctt tttcttcgcg ttgtacagga
     7261 ggagcgtcgc accattaccg cgatcgtaaa cttcacgttt atggcggata acttcgccat
     7321 ctttgcgcgt gagatcgtaa gtaatgttgt gcagggtgaa atagttatcg gagagaattt
     7381 tgtctttaac gagggtgatt tgttgcgtca taccgactcc acagcgcgaa atgaacaatt
     7441 atcttacgct gtgaagtcgg gtttgtctgc gcaggctatc agtgagattt gactgatttt
     7501 acgcccagcc agtcaatgat cccctgtgcc gcatgacgtc cttctgccat tgcggtaacc
     7561 accagatccg caccacgcac ggcgtcacca ccggcgaaga ttttcggatt cgtggtctgg
     7621 taacggtact ggctttccac atccgcgatg atgcggcccc atttgtctac cgttacaccg
     7681 tgcgactcca gccacggcat cccgtgcgga ttgaagccaa acgccataat caccgcatcg
     7741 gctggcatga caaattcact gccttccacc ggcactggac gccgacgccc ctgggcatcc
     7801 ggctcgccaa gacgcgtgcg caggaaacga atcccgcaga cgtgaccttg ttcattcagt
     7861 tcaagcgcca ccggctggac gttaaattcg aagttggccc cctcttcacg ggcgttcttc
     7921 acctctttct tcgagcctgg catgttagct tcatcacgac gataagcgca ggtgacgtta
     7981 ctcgcgccgt ggcgcagcgc agtacgcaca cagtccatcg cggtgtcgcc gccgcccagt
     8041 accacgacgt taagtccggc agtattgata aacggctctt ccggtagctc ttcgagtccc
     8101 atcacctgtt tagtattggc gatgaggaac ggcagcgcgt cataaacgcc cggcgcatct
     8161 tcattgggta aacccgcttt catggagcgg taagtgccta cgccaacgaa gaccgcgtcg
     8221 tattgttcca aaagcgaatt caaagagaca tctttaccca cttcacaatt gagttcgaag
     8281 tgaatcccca tcgcgctgaa gatttcccga cggcgtgcca gcagggattt atccagtttg
     8341 aaagaaggaa tgccgaaagt gagcaagcca ccgatttctg gatggcgatc gtacaccgtc
     8401 accgccacgc cattgcgggt gagaacatct gcacaggcca gccctgccgg acccgcaccg
     8461 ataatcgcca cccgcttgtc cactttggtg acatggctta agtcaggacg ccaacctttc
     8521 gccaacgcct gatctgaaat gtagcgttca atgttaccga tagttactgc gccgtgctca
     8581 tcgcgaatag tacaggcacc ttcacacaaa cggtcttgcg ggcaaacgcg tccggtaatt
     8641 tccggcaggg tgttggtctg gtgagaaagc tcgacggcgg cgtcgatgtt tccggctttc
     8701 accagttcaa tccactgcgg tatatggtta tgcagcgggc aggtccattc acaaacgctg
     8761 tgctcgccac acttaaggca gcgcgaggct tcccgttgtg cctgatcggc acggaatggc
     8821 agataaattt catcaaaacc ggttttgcgc gcttcaatcg ccagtttatc tggctcgcca
     8881 cgcgcgggcg ttgcctgcat ttgctcgact ttactcatta ccggcatttc ttgcgccgcg
     8941 gtactggcat gccacggttg ttgttcctga cgtgcggtgc gcaagcggcg ggatttcgcc
     9001 atgccggaga gtgcggcgtc agtgaccagt tgcagcgcgt ccgccgggca attttcgaca
     9061 caggcaggac cgttttcgcg ccctgcacaa aggtcgcatt tatgcgccgt ggcttttact
     9121 tttcctgccg cgacgggtgt cagaacgatt tgcatcgtac caaatggaca ggccaccacg
     9181 caggatttac agccaataca cttttgctga ttgacctgaa tgctgtcatc aacgtggctg
     9241 attgcgccat tagggcagct acgggcgcag ggcgcatctt cacaatggtg acaggtcact
     9301 gcactacgtt gctgctgatg tttgataacc gtaattcggg gatgaaaatg gcgttggctc
     9361 aggacatgtt gctcgtcatt gtgagccatg acacaggcga tttcacaagc atgacacccc
     9421 agacattgct gactgttggc cataataaaa cgattcataa cgaccttctt ttttggttgt
     9481 aaaaacctta ttctttatat gagtgttgtt attacccgac ttacagggga atcggcaatg
     9541 ttcatgtgcc cagaataagt aactatttcg ctataaactg tggcagatca aataatccca
     9601 tcactgacta aattgcgttt cagcgaactg gaacattaat gattttttgt ggagaagacg
     9661 cgtgtgattg ttaaacgacc cgtctcggcc agtctggccc gggccttttt ttacattgtg
     9721 ctgctgtcga ttctttccac gggtatcgct ctgctaacgc tggcgagcag tttacgcgac
     9781 gctgaggcta tcaatattgc cggatcgctg cgtatgcaga gttaccgcct gggctacgac
     9841 ttgcaaagtg gcagtccaca actcaatgca catcgccagt tatttcagca ggcactgcat
     9901 tcaccggtat taaccaatct caacgtctgg tatgtgccag aggcagtaaa aactcgctat
     9961 gcgcatctga atgccaactg gctggagatg aatcatcggc tcagcaaggg cgacttgccg
    10021 tggtatcagg ccaatattaa taattatgtt aatcagatag acctgttcgt gctggcttta
    10081 cagcactacg ctgaacgtaa aatgctgctg gtggtggcga tttccctggc tggcggcatc
    10141 ggtattttca cgctggtctt ttttactttg cgccgcatac gccatcaggt ggttgccccg
    10201 ctgaatcagc tggttaccgc cagtcagcgt attgaacacg ggcagttcga ctcgccgccg
    10261 ctggatacca gcctgccgaa tgagcttggc ctgcttgcaa aaacctttaa ccagatgtcg
    10321 agcgagctgc ataaattgta ccgttcgctg gaggcgtcag tagaagaaaa gacccgcgat
    10381 ctccacgagg ccaagcgtcg tctggaggtg ttgtatcagt gttcgcaggc gctaaacacc
    10441 agccagattg atgtgcattg tttccgccat attttgcaga ttgttcgcga caatgaagcg
    10501 gctgaatatc tggagttaaa tgtcggtgac aactggcgga ttagcgaagg gcagccaaac
    10561 ccggaattgc cgatgcagat tttaccggtg acaatgcaag agacggttta cggcgaactg
    10621 cactggcaaa atagtcacgt ttcatcatca gaaccgctgc ttaacagcgt ttcgtcgatg
    10681 ctgggacgcg gtttgtactt taatcaggcg cagaagcatt ttcagcagtt attgttgatg
    10741 gaagaacgtg cgaccatcgc ccgcgaattg cacgactcgc tggctcaggt actttcttac
    10801 ttacgtatcc agttgacgtt actgaagcgt tcgataccgg aagataacgc caccgcacaa
    10861 agtatcatgg ccgatttttc ccaggcgttg aatgatgctt atcggcagtt acgcgagctg
    10921 ttgactactt tccgcctgac gctgcagcag gcggatctcc cctccgcgtt gagggaaatg
    10981 ctggatacgt tacaaaatca aaccagcgcc aaactgaccc tcgactgccg tctgccaacc
    11041 ctggcgctgg atgcgcaaat gcaggtgcat ttgttgcaaa ttattcgcga agcggtgctg
    11101 aatgcgatga agcacgccaa cgccagcgaa atcgccgtta gctgcgtcac cgcgccggat
    11161 ggcaatcaca cagtctatat tcgcgacaac gggattggca tcggtgaacc gaaagaaccc
    11221 gaaggccatt atggtctgaa tatcatgcgc gaacgcgcgg aaagattggg tgggacgctg
    11281 actttttcac aaccttccgg cggcggcacg ttagtgagta ttagctttcg ctctgcggag
    11341 ggtgaggaaa gtcagctgat gtaatgcctc ttattgacca aagaatagtg gcacttaagg
    11401 ttcagtataa aagggcatga taatttacat taactccttt ttttctccac gattggctcg
    11461 taccttgccg ctacagtgaa gcaagccaag cctacaacga tacgcagaaa cacgaggtcc
    11521 tcttttaatg gcgaatttct ttattgatcg ccccattttt gcctgggtgc tggcaatcct
    11581 gttgtgtctg acaggtaccc tggcgatttt ttcattgccc gttgaacaat accccgatct
    11641 cgcgccaccg aatgtgcgag tgaccgctaa ctatcccggc gcatcggccc agacgctgga
    11701 aaacaccgtg acccaggtta tcgagcaaaa tatgaccggc ctcgataatc tgatgtatat
    11761 gtcatctcag agcagcggca ccggtcaggc atctgtcacg ttaagtttta aagcaggcac
    11821 cgatccggac gaagccgtgc agcaagtaca aaaccagctg caatcagcca tgcgaaagtt
    11881 accgcaggcg gtgcaaaatc agggcgtgac ggtgcgtaaa accggcgata ccaacattct
    11941 gaccattgcc ttcgtctcta ccgatggttc gatggataaa caggatattg ccgattatgt
    12001 tgccagtaat attcaggacc cgttaagccg cgtgaatggc gtcggggata tcgatgccta
    12061 tggttcgcaa tattccatgc gtatctggct ggacccggcg aaactcaaca gtttccagat
    12121 gacggctaaa gatgtcaccg atgccattga atcacagaac gcgcagattg cggttgggca
    12181 acttggtggt acgccttccg tcgataagca ggcgctcaac gccaccatta acgcccagtc
    12241 actgctgcaa acaccagaac agttccgcga tatcaccttg cgggtgaatc aggacggctc
    12301 agaggtaagg ctgggcgatg tcgccaccgt cgaaatgggg gcggagaaat acgattatct
    12361 tagccgcttc aatggtaagc cagcctccgg gctgggggtt aaactggcct ccggcgcgaa
    12421 cgaaatggcg acagcggagc tggtgctcaa tcgtctcgac gagctggcgc agtattttcc
    12481 acacggactg gaatacaagg tggcgtatga aaccacctcg tttgttaaag cctccattga
    12541 agacgtggtg aaaacgcttc tggaagctat cgccctggtt ttcctcgtta tgtatctgtt
    12601 cctgcaaaac ttccgcgcca cgctgatacc cactatcgcc gtgccggtgg tgttgatggg
    12661 aaccttctcc gtgctttacg ccttcggtta cagtgtcaac accttaacca tgttcgcgat
    12721 ggtgctggcg atcggtctgc tggtggatga tgccatcgtg gtggtggaaa acgtcgaacg
    12781 tattatgagt gaggaaggac tcactcctcg cgaagccaca cgtaaatcga tggggcagat
    12841 ccagggggca ctggtcggga ttgcgatggt gctttcggcg gtatttgtgc caatggcctt
    12901 cttcggcggc accaccgggg ccatctatcg ccagttctct attaccattg ttgcggcgat
    12961 ggtgctgtca gtactggtag cgatgatcct cactcctgct ctgtgtgcca cgctacttaa
    13021 gccactgaaa aaaggtgagc atcacgggca aaaaggcttt tttgcctggt ttaaccagat
    13081 gtttaaccgc aactccgaac gctacgaaaa aggggtggcg aaaattctcc accgtagcct
    13141 gcgctggatt gtgatttatg tcctgctgct tggcggcatg gtgttcctgt tcctgcgttt
    13201 gccgacgtcg ttcttaccgc tggaagaccg tggcatgttt actacctcgg tacagttgcc
    13261 cagcggttca acccagcaac agaccctgaa agtcgttgag caaatcgaga aatattactt
    13321 cacccatgaa aaagacaaca tcatgtcggt gtttgccacc gtgggttctg gccctggggg
    13381 taacgggcaa aacgtggcgc gaatgtttat ccgcctgaaa gactggagcg aacgcgacag
    13441 taagaccggc acctcgtttg ccattatcga gcgtgcaacg aaggcgttta acaagattaa
    13501 agaagctcgc gttatcgcca gcagcccgcc agcaattagc ggtcttggta gttctgcggg
    13561 ttttgatatg gagttgcagg accacgctgg agcgggtcac gatgcgctga tggcagcacg
    13621 aaaccagttg ctggcgctgg cagcggaaaa cccggagcta acccgtgtgc gccataacgg
    13681 cctcgacgac agtccgcagt tgcagattga tatcgaccag cgtaaagctc aggcgctggg
    13741 cgttgctatc gacgatatta acgacacact gcaaaccgcc tggggttcga gctatgtgaa
    13801 tgactttatg gatcgcggtc gcgtgaagaa agtctatgtg caggcagctg cgccgtatcg
    13861 catgctgcca gatgacatca atctctggta tgtccgaaat aaagatggcg gcatggtgcc
    13921 cttctctgct ttcgcgacct cacgctggga gacaggctcg ccgcgtctgg aacgctataa
    13981 cggttattct gcggttgaga ttgttgggga agccgcaccg ggggtcagta ccggtacggc
    14041 gatggatatt atggaatcgt tagtgaagca gttgccaaac ggctttggtc tggagtggac
    14101 ggcgatgtcg tatcaggagc ggctttccgg cgcgcaggct ccggcgctgt acgccatttc
    14161 cttgctggtg gtattcctgt gtctggctgc gctgtatgaa agctggtcgg tgccgttctc
    14221 ggtaatgctg gtcgtgccgc tgggggttat cggcgcgctg ctggcaacct ggatgcgcgg
    14281 gctggaaaac gatgtttact tccaggtggg cctgttaacg gtcattggtt tatcggcgaa
    14341 aaacgctatc ctgatcgtcg agtttgctaa cgagatgaac caaaaaggcc acgacctgtt
    14401 tgaagcgacg ctccacgcct gccgtcagcg tttacgcccg attctgatga cctcgctggc
    14461 atttatcttc ggcgtattgc caatggcaac cagcacaggt gccggttccg gtggtcagca
    14521 tgcggtgggt actggcgtaa tgggcgggat gatttcggcc actattctgg ctatttactt
    14581 cgtgccgctg ttctttgtgc tggtgcgccg ccgcttcccg ctgaagccgc gcccggaata
    14641 agcaataaaa aaggcgacat gccaatgtgt cgcccttttc aactttccga ttaagaacct
    14701 gctcagcggg ttcttgctgt ttgtactttg tctcaggaat tacttacgaa gcataacttc
    14761 gataaagtct ttccagttcc ccagttcacg ttcaatcata acaacctctc ttataattat
    14821 gggtattcta cggaaacaat ataccgtggt gaagctaatt tactcgattg ctgtgatgac
    14881 tacctccggg ggacaaacct tatgtaaata ctatggtcct gcggtgatga tttgtatgtg
    14941 atgcaccgca acatttcgag atattcatac ggcatctaat actgatttaa ttctggtta
//
LOCUS       DADTFG010000121        14946 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.122, whole genome shotgun sequence.
ACCESSION   DADTFG010000121 DADTFG010000000
VERSION     DADTFG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.122"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            190..1308
                     /locus_tag="J1138_003460"
     CDS             190..1308
                     /locus_tag="J1138_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA8455219.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGETIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKDPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGLGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILSGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVI"
     gene            1351..1475
                     /locus_tag="J1138_003461"
                     /pseudo
     CDS             1351..1475
                     /locus_tag="J1138_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1529..1813)
                     /locus_tag="J1138_003462"
     CDS             complement(1529..1813)
                     /locus_tag="J1138_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455220.1"
                     /translation="MLDRHCKVYIACSSIINLVNCETKQRTLFERIYFSQYWAKGDVI
                     AKRAPISQWEPYSEESLLVIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            1790..1894
                     /locus_tag="J1138_003463"
                     /pseudo
     CDS             1790..1894
                     /locus_tag="J1138_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1931..2416)
                     /locus_tag="J1138_003464"
                     /pseudo
     CDS             complement(1931..2416)
                     /locus_tag="J1138_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            complement(2540..2860)
                     /locus_tag="J1138_003465"
     CDS             complement(2540..2860)
                     /locus_tag="J1138_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455221.1"
                     /translation="MDIWPEFQRDLEMYRDVVLSIKRNLRLYEECIESLVHQIGSTNF
                     DNAQPLFDDLFRMQSELATMLYKYEYKPGKRIQDLIYHLDRDDFYSRKYWHKKFSDGL
                     AWPE"
     gene            complement(2874..12737)
                     /locus_tag="J1138_003466"
     CDS             complement(2874..12737)
                     /locus_tag="J1138_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HBA8455222.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARD
                     GDITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVSSRSDIVLSNGTLNS
                     DKDLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAARDIVTVASDTLTTQG
                     QITAGQNLTASATTLTQDGILLAKGHAGLDAGTLNNSGAVQGASLTLGSTTLSNSGSL
                     LSGGPLTVNTRDFTQSGRTGAKGKVDITASGKLTSTGSLVSDDVLVLKAQDVTQNGVL
                     SGGKGLTVSAQALSSGKKSVTHSDAAMTLNVTTVALDGENSAGDTLRVQADKLSTAAG
                     AQLQSGKNLSINARDARLAGTQAAQQTMAVNASEKLTHSGKSSAPSLSLSAPELTSSG
                     VLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSG
                     LITGDNGLTLNTASLSNPGKIIADTLNVRATTLDGDGLLQGAGALALAGDTLSQGRNG
                     RWLTAGDLSLRGKTLHTAGTTQGQNLTVQADRWANSGSVLATGNLTASATGQLTSTGD
                     IMSQGDTTLKAATMDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTIRQNSVTNSGTLT
                     GIAALTLAARMASPQPALMNNGGSLLTSGDLTITAGSITSSGHWQGKRVLITADSLAN
                     SGAIQAADSLTAHLTGELVSTAGSKVTSNGEMALSALNLSNSGQWIAKNLTLKANSLT
                     SAGDITGVDTLTLTVNQTLNNQANGKLLSAGVLTLKADSVTNDGQLQGNATTITAGQL
                     TNGGHLQGETLTLAASGGVNNRSGGVLMSRNALNVSTATLSNQGTIQGGGGVSLNATD
                     RLQNDGKILSGSNLTLTAQVLANTGSGLVQAATLLLDVVNTVNGGRVLATGSADVKGT
                     TLNNTGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQHGTGRLYSAGNLLLDAQ
                     DFSGQGQVVATGDVTLKLIAALTNHGTLAAGKTLSVTSQNAITNGGVMQGDAMVLGAG
                     EAFTNNGMLTAGKGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGSLT
                     MNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWVQKDASGGANTEIINTSG
                     NIETHQGDIVVRTGHLLNQREGFSATTTTRTNPSSIQGMGNALVDIPLSLLPDGSYGY
                     FTREVENQHGTPCNGHGACNITMDTLYYYAPFADSATQRFLSSQNITTVTGADNPAGR
                     IASGRNLSAEAERLENRASFILANGDIALSGRELSNQSWQTGTENEYLVYRYDPKTFY
                     GSYATGSLDKLPLLSPEFENNTIRFSLDGREKDYTPGKTYYSVIQAGGDVKTRFTSSI
                     NNGTTTAHAGSVSPVVSAPVLNTLSQQTGGDSLTQTALQQYEPVVVGSPQWHDELAGA
                     LKNIAGGSPLTGQTGISDDWPLPSGNNGYLVPSTDPDSPYLITVNPKLDGLGQVDSHL
                     FAGLYELLGAKPGQAPRETAPSYTDEKQFLGSSYFLDRLGLKPEKDYRFLGDAVFDTR
                     YVSNAVLSRTGSRYLNGLGSDTEQMRYLMDNAARQQKGLGLEFGVALTAEQIAQLDGS
                     ILWWESATINGQTVMVPKLYLSPEDITLHNGSVISGNNVQLAGGNITNSGGSINAQNG
                     LSLDSTGYIDNLNAGLISAGGSLDLSAIGDISNISSVISGKTVQLESVSGNISNITRR
                     QQWNAGSDSRYGGVHLSGTDTGPVATIKGTDSLSLDAGKNIDITGATVSSGGTLGMSA
                     GNDINIAANLISGSKSQSGFWHTDDNSASSTTSQGSSISAGGNLAMAAGHNLDVTASS
                     VSAGHSALLSAGNDLSLNAVRESKNSRNGRSESHESHAAVSTVTAGDNLLLVAGRDVA
                     SQAAGVAAENNVVIRGGRDVNLVAESAGAGDSYTSKKKKEINETVRQQGTEIASGGDT
                     TVNAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTH
                     TISEDSASREAGSLLSGNRVTVNAGDNLTVEGSDVVADQDVSLAAGNHVDVLAATSTD
                     TSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAG
                     KQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAIN
                     NAVTSAQETKESSDSRLKALQATKTALSGVQAGQAATMASATGDPNATGVSLSLTTQK
                     SKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDAAN
                     DVLLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVNAAKGSEKGNGTEWT
                     ETTIDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSVAA
                     GGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDITVGRHTQLDGAVIA
                     STATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMISAGG
                     HSGHAEGTTQAAVAEGTITIRDRDNQKQNLANLSRDPAHANDSISPIFDKEKEQRRLQ
                     TVGLISDIGSQVADIARTQGELNALKAAQDKYGPVPADATEEQRQAYLAKLRDTPEYK
                     KEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPELAHLLKSTEKDPAVNA
                     IAHAILGGTVAAMQGNNVAAGAAGAATGELAARAIAGMLYPGVKQSDLSEEQKQTIST
                     LATVSAGLAGGLTGNSTASAAVGAQSGKNAVENNYLSVSEKTELEIAKQTLKNSKDPA
                     EREKAQQKYDALLEKDIASDKEVIAACSNGNASSSACASARLKVIASKEGYEDGPYNS
                     KYSQQYADAYGQIVNLLDITSVDAQNQQQVKNAMINYFMVTKGVDRQTAESYTETTQG
                     LEIIAASVTPLIGQAASNKLSYLGIGKKISFDGDFYTVDGMKFSKSYYEKLWEQGRPA
                     PFVQAREVLNSNPKIEPDPRGAPGYLRYEGAGLEMIYNPKTGQVGHIQPVKVK"
     gene            complement(12750..14522)
                     /locus_tag="J1138_003467"
     CDS             complement(12750..14522)
                     /locus_tag="J1138_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HBA8455223.1"
                     /translation="MQRRQNNILTITSLLSRALSVPCCDMFRRSSPWICYLSLSVFSG
                     FFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYITS
                     RAFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPGRTLKMVFPGMEGKVLNLRDIEQG
                     MEQINRLRTESVQIEISPGDREGWSVVTLTALPEWPVTGSVGIDNSGQKNTGTGQLNG
                     VLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYLST
                     IDNRGWLWRSTGDLQTHRLGLSHVLFRNGNMKTALTGGLQHRIIHNYLDDVLLQGSSR
                     KLTSFSVGLNHTHKFLGGVGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSVSAS
                     FQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRNEL
                     SWSLFSLPYVGTVRAVTALDGGWLHSDRDDPYSSGTLWGAAAGLSTTSGHVSGSFTAG
                     LPLVYPDWLAPDHLTVYWRVAVAF"
BASE COUNT         3298 a         4095 c         3720 g         3833 t
ORIGIN      
        1 ttataactta ggggcaactg gaggaatggc cgcacctgta ttagctacat acatttctgg
       61 atattatggc ttaggtgttt cattattcat tgttacggtt gcattctctg ccttattaat
      121 tttgttagtt ggttttgata ttccaggtaa aatttataaa ctatccgtgg ctaaataatt
      181 ggaggcttta tgattaatta tggcgttgtt ggtgttggat actttggcgc tgaattagct
      241 cgttttatga atatgcatga taatgcaaaa attacatgtg tatacgatcc tgaaaatgga
      301 gaaactattg cccgtgaatt gcaatgtatc aatatgtcaa gcttggatgc tttagtttca
      361 agtaaattag tcgattgtgt gatcgtagcc accccaaatt atctgcataa agatccagta
      421 attaaagcag caaagaataa gaagcatgtt ttttgtgaaa aaccaattgc attaagttat
      481 gaagattgtg tggatatggt caaagcgtgt aaagaagctg gtgtgacctt tatggctggg
      541 catattatga attttttcaa tggagttcaa tatgcacgga agttaattaa agaaggtgtt
      601 atcggcgaaa tattatcatg tcataccaag agaaatggct gggaaaacaa acaggagaga
      661 ctttcctgga aaaagatgaa agaacaatct ggtggacatc tatatcatca tatacatgag
      721 ttagattgtg ttcagcattt acttggagaa ataccagaga cggttactat gattggtgga
      781 aatttagctc attctggtct agggtttggc aatgaagatg atatgttatt tatgaccttg
      841 gaattcccgt caggaaaact agcaacctta gagtggggga gtgcatttaa ctggccagaa
      901 cattatgtca tcatcaatgg aactaaaggc tctattaaaa ttgatatgca agaaacagca
      961 gggtcactta ggattggcgg tcagacaaag cattttttgg tccatgaaac acaagaagaa
     1021 gatgatgatc gtcggaaagg caatatgacc tcagaaatgg atggcgctat agcatatggt
     1081 catcctggaa aaaaaacacc attatggctt gccagtttaa taagaaagga gacgttattc
     1141 ctccataata tcctcagtgg tgcaaaacct gaagaagatt atattgacct tctcaatggt
     1201 gaggcggcca tgtcggcgat tgctactgct gatgctgcca ctctttcaag atcgcaggac
     1261 aggaaagtga aaatcagtga gatcattaaa catacatcag taatataaaa actatacgcc
     1321 tcttgattta attcaggagg ccttttatgt atgatcaaga attttatctt cgataatctc
     1381 ataattttag cagcgccatt tatgataaaa aatcccttaa aaccaatcta atctttttct
     1441 tcctctgtgt atttgttccc cgtatggcgt cataatttat gcatacgtga tgcgggatag
     1501 ttacaatatg cagcgatagc gctaagtttt agttaaaatc ccccattagc gggatatggg
     1561 gatctctggg aggttcaggt tttagcatcg cgactttaat acgacagacg ctggtgacga
     1621 tgatgacaag cagtgactct tccgaataag gttcccactg actaattgga gcgcgcttcg
     1681 caataacatc tcccttcgcc cagtattggg aaaagtagat acgttcaaac agtgtacgct
     1741 gtttcgtctc acagttcacg agattgatga tcgatgaaca ggcaatataa accttgcagt
     1801 ggcgatcgag cattacaatg aaaaccatcc gcatagtgcg ttgggttatc gctctccgcg
     1861 agaatatcga cgtcagcggg taatgttaac ttaagataca gcccctgtct ggaaataagg
     1921 gggtaagaac acccgcatcg gtaactatta tgactctggc cacagggttt acagcctcgg
     1981 caagggcatc tgggaagttt ttttgtatct gcgagttctg ctgtttttct gacgggacta
     2041 cccagcttaa caggggaagc gagcatccat cgcagagcag gctggcgcgg agcacatgat
     2101 gctcctgaga tgaatatcca ctccagtcaa cggcgatgac acaaagagat agctggctag
     2161 tcagcatggc aataatatta ttgaaaatca gtgggatatc gcggtgaaga gattcattac
     2221 cgagcagtcg gtcaacgcgt ttgattttat ttttgacccg agccaagccg ggtaaataac
     2281 gcccgatgct ggtcaacgtc agtgatgcgc cattgatgag agcgatggtg gcatccagca
     2341 aagcattttg tcggtatttg tgaaacggtg ctaacgcatc gtggaaaaaa ttctgacata
     2401 cttgtcgagc aggcatagaa gcgatctcat tgaattggtt gcacaaatca gtagatcaca
     2461 aaactctatg cctgtctttt ttacccgccc attactaggg attcctcagc gctaactgag
     2521 ggcgggattt ctacttctat tattcaggcc atgccaatcc atcactgaat tttttatgcc
     2581 aatattttct tgagtaaaaa tcatccctgt ccagatgata tattaaatct tgtatccttt
     2641 tgcctggctt atattcatat ttgtataaca tagtagcaag ttcagattgc atacgaaaga
     2701 ggtcatcaaa tagaggctgc gcattatcaa aattggtgct tccgatctga tgaactaacg
     2761 attcaataca ctcctcatac aaccgaaggt tacgttttat tgataaaaca acatctcgat
     2821 acatctcaag atctcgttga aattcaggcc aaatatccat ttacttcctc tttttattta
     2881 accttgactg gttgaatatg accaacttgt cccgttttcg ggttatagat catttcaaga
     2941 cctgcacctt catatcttaa gtagccaggt gcgcctctag gatccggctc aatttttgga
     3001 ttgctattca acacttctct tgcttgcacg aatggcgcag gtctgccttg ctcccataat
     3061 ttctcataat aacttttgct gaatttcatt ccatctacag tataaaaatc accatcaaag
     3121 gatatctttt tcccaatacc aaggtagctt aatttatttg atgcagcttg ccctataaga
     3181 ggcgttaccg aagccgcaat gatctccaag ccctgcgttg tttcggtgta gctttctgcg
     3241 gtctgacgat ccaccccttt ggtgaccatg aaatagttaa tcatggcgtt tttcacctgc
     3301 tgctgattct gtgcatcaac actggtgata tcgaggagat tgactatctg accatacgca
     3361 tcagcgtatt gctggctgta tttcgagtta taagggccat cttcgtaccc ttctttcgac
     3421 gcaataactt tcagtcttgc gctggcacac gctgaactac tcgcattacc attactacag
     3481 gcagcaataa cctccttatc actggcaata tctttttcaa gcaatgcatc atatttctgc
     3541 tgtgcctttt cacgctctgc cggatcttta ctgtttttaa gcgtctgttt tgctatctca
     3601 agttctgtct tttcagacac gctcaggtag ttattctcaa cagcattctt cccgctctgt
     3661 gctcccactg ctgctgatgc ggtgctgttt ccggttagac cgccggccag ccctgctgac
     3721 acagttgcca gcgtacttat cgtctgcttc tgctcttcac tgaggtctga ctgcttcact
     3781 cccggataca gcattccggc aatcgctctg gctgccagct ctcccgttgc tgcgcctgct
     3841 gcaccagcag caacattatt accctgcatt gccgctactg taccgcccag aatggcgtga
     3901 gcaatggcgt ttactgccgg gtctttttct gttgatttca gcagatgcgc cagttccggt
     3961 gcagaagctc cggccagagc acccgctaaa ttaccacccg ccagtccctg aagcgcggct
     4021 gtcgcggcct gtatacctcg ctgaatctca ctgccagtgc catacttttc ctgttccttt
     4081 ttgtattccg gcgtatcacg cagttttgcc agatatgcct gccgctgttc ttccgtcgca
     4141 tccgccggaa caggcccata tttatcctgc gcagccttca atgcattcag ttccccctgc
     4201 gtccgcgcga tatccgccac ctgactgcca atgtcactga taagccccac tgtctgcaga
     4261 cgcctctgct ctttctcctt gtcaaatatc gggctgatac tgtcattagc gtgcgcaggg
     4321 tcacggctca ggttcgccag attctgcttc tgattgtccc tgtcccggat ggtgatagtg
     4381 ccttcagcca ctgcggcctg agtcgttcct tccgcatgtc cgctgtgacc tccggcggat
     4441 atcatgccac ccggcatgtt accctgaaat ttatccccga agctgccacc accgctcaga
     4501 ctgattccac tgtgactgac tttataatcc gcttcgttgt gaaggtcact gaaccccagc
     4561 gttccggtat ccaggtggtt tttatccggt gtggcagtgg aggcaatcac cgcaccatcc
     4621 agttgggtat gtctgcccac tgtaatgtcg aagccgccgt caccggcaaa cattccggtt
     4681 tgttcagcaa cggagtcaaa gcggctcttc atcttatccc gggaggcagc gatgtaacct
     4741 gagccggtca tggagccaaa ggtaaaactg ccgccggcag ccacgctggt ctgtttactg
     4801 tcgtacttac tggtgtcctg ctggctgctt atcagcaggt cgtggcccac atcggcgata
     4861 atcctgttgc cgttgacctg agcaccgttc agtaccgtat cccgaccact gttgatggtg
     4921 acggttttac cgctatctat tgtggtttca gtccactcag taccgttacc tttctcgctg
     4981 ccttttgccg cattaacgct ggcaaagacg ctgataccgg caccgttacc accggcaccg
     5041 atactgacac ccacgccacc gccactgctg ctgttcctgc ccgttgtttt ttgtgtgttt
     5101 gcagcgccac tcaacagaac atcattcgcg gcatccaggc ttgtgttacc accggcctta
     5161 agctggcttc cggcaatcac aatatctccg cggttatcgc ccctgttttt accggttgcg
     5221 acaacagaca gattattccc ggcattcagc gtactgccgg atactgtgtc actttcagaa
     5281 tgttgctgtg atttcgattt ctgggtggtg agcgacaggc tgactcccgt cgcattcggg
     5341 tcaccggttg cggaggccat tgtcgcagcc tgtccggcct gcacaccaga cagcgctgtc
     5401 tttgtggcct gcagggcttt cagacggctg tcactgctct ccttcgtctc ctgtgcactg
     5461 gtaaccgcat tattgatggc actgcccacc gtgccggaaa gggcaaccgt cagcccgctt
     5521 ttcttctgct caaatttttc gtccacagta cgacggtcat gccccgggtc aaccaccaca
     5581 ctgtcaccgg taatgctgat atcccggttc gcaatcacat ccgaaccgct gatatgagcc
     5641 tgtttgcccg cggtaatact gacattaccg gcagtggagc cgatggtgct ggcactctga
     5701 ctctgcgttg tcccggcctc gcgccggtcg tgcgttgtct tactgctgcc aatggtgaaa
     5761 ccaataccgc cggtacccat cagaccggat ttcttcgttt ccttaaagcg ccaggacgta
     5821 tctgtactgg tggcagcaag aacatcaaca tggttacccg ccgccagtga cacatcctgg
     5881 tcagccacca catccgaacc ctctaccgtc aggttatcac cggcgttaac ggtcacgcgg
     5941 ttccccgaca gcagggagcc tgcttcacgg gaggcactgt cctcactgat ggtgtgggtg
     6001 gttttcttac tgagaaaacc tccgcttttt ttcttcgttt ccagatagtg atagtcactt
     6061 tctgtcgccg tggtcagggc aacatcacga ccggcattca cgctgatatt gccggttgcg
     6121 gtaacggatg acgcaacagc ggtgatatcc cgtcctgcgt tgacggtggt gtcaccaccg
     6181 ctggcgattt ccgttccctg ctgacggact gtctcgttaa tctctttctt tttcttcgac
     6241 gtatagctgt cgcctgcacc ggcagactct gccaccaggt tcacatcacg tccgccccgg
     6301 atgaccacgt tattttccgc agccacaccg gcagcctgac tggcaacatc acgaccggca
     6361 acaaggagga ggttatcgcc cgccgtcacc gtggacactg ctgcgtggct ttcatgactt
     6421 tctgacctgc cgttgcgact gtttttgctt tccctgactg cattcagact caggtcgtta
     6481 cctgcagaaa gcagggcgct gtgcccggca gaaacagagg atgccgtgac atccagatta
     6541 tggcctgcag ccatcgccag gttaccgccg gcgctgatgc tgctgccctg tgaggtggtg
     6601 gatgatgcac tgttgtcatc agtgtgccag aaaccggact gacttttgct cccgcttatc
     6661 aggtttgcgg caatgttgat atcattaccc gcagacattc caagggttcc accggacgag
     6721 accgttgccc cggtaatatc aatgtttttc cctgcatcca gtgaaagtga atcagtgcct
     6781 ttaatggtcg caaccggacc ggtgtccgta ccgctgagat gcacaccacc atatcggctg
     6841 tcactgcccg cattccattg ctgacgccgg gtgatattgc tgatgttgcc actcacgctt
     6901 tccagttgta cggttttacc gctgatgact gagctgatat tgctgatatc cccgatggcg
     6961 ctcaggtcta ggctaccgcc cgcgcttatc agccccgcat tcaggttgtc gatatagccg
     7021 gtactgtcga gcgagaggcc gttttgtgcg ttgatgctgc cgccgctgtt ggtgatattg
     7081 ccacccgcaa gctgcacgtt gttcccgctg ataacgctgc cgttatgcag ggtgatatct
     7141 tccggcgaca gatacagttt cgggaccatg actgtctgtc cgttgatggt ggctgactcc
     7201 caccacagta tgctgccgtc aagctgagca atctgttcag ctgtcagcgc cacaccaaac
     7261 tctaatccca gtcctttctg ttgtctggcc gcgttatcca tcagataccg catctgttcc
     7321 gtgtctgaac ccagtccgtt gagataacgt gaacccgtcc ggctcagcac cgcgttactg
     7381 acataccggg tatcaaagac cgcatccccc aggaaacgat aatctttttc cggtttcagc
     7441 ccgaggcggt caagaaaata cgatgagccc agaaactgtt tttcatcggt atacgacgga
     7501 gccgtttcac gtggcgcctg acccggtttc gctccaagaa gctcatacag tccggcaaac
     7561 aaatggctgt ccacctgtcc gagaccatcc agtttcgggt tcaccgtaat cagatacgga
     7621 ctgtccgggt ccgtggacgg aaccaggtat ccattgttgc cggaaggcag tggccagtca
     7681 tcactgatac cggtctgacc ggtcagtggc gaacctccgg caatattttt cagggcacct
     7741 gccagttcat cgtgccattg cggagagcca accaccaccg gctcatactg ctgcagcgct
     7801 gtctgtgtca gactgtctcc gccggtctgc tgacttaacg tattcagtac cggtgcagag
     7861 accaccggac tgacactacc tgcatgtgca gtggttgttc cgttattgat actgctggta
     7921 aaacgggtct taacatcccc gcccgcctga ataacggaat aatacgtctt accgggcgtg
     7981 taatcttttt cccggccatc cagtgaaaat ctgatggtat tgttttcaaa ttccggtgac
     8041 agcaggggca gtttatccag agagcctgtg gcatagctac cgtaaaacgt tttcgggtcg
     8101 tagcggtata ccagatattc attctctgtc cccgtctgcc agctctgatt gcttaactct
     8161 ctgcccgaaa gtgcgatatc cccattcgcc aggataaatg acgcccggtt ttccagtcgt
     8221 tcagcctcag cagaaagatt acgccctgac gcaatgcggc ctgccggatt atcagcaccg
     8281 gttactgttg tgatgttctg gctgctgaga aagcgctgtg tggcactgtc agcaaacgga
     8341 gcgtaataat aaagcgtatc cattgtgata ttgcatgccc cgtgcccgtt gcagggcgta
     8401 ccgtgctgat tttcaacttc acgggtgaaa tagccatagc tgccgtcagg aagaagggaa
     8461 aggggaatat caaccagagc atttcccatt ccctgaatgg atgaggggtt agtccgggtt
     8521 gttgttgtgg cagaaaatcc ctcccgctgg ttcagaagat gcccggtcct tacaacaata
     8581 tcgccctgat gcgtctcaat attcccggag gtattgataa tctctgtgtt tgcaccgccg
     8641 gaagcatcct tctgtaccca cagactgttg ccggccagga tatcaccatg ctggttatgc
     8701 agacggtctg taaacagctt caggttattc cccgcataaa tcagcgcact gttcagcagg
     8761 gtaccggcca cattcattgt cagactgcct gccgtgccgg taaaaccact gatggtgata
     8821 tcactccggc tgttcagact cacatcgcca ccggcctgaa gtgaacccgg tgcgttaagg
     8881 aaaagacgct gtgcgctgaa aacactgttg cctttaccgg cagtcagcat tccattgttg
     8941 gtgaatgcct ctccggcacc gagcaccatg gcatcaccct gcatcacacc gccgttggtg
     9001 atggcatttt gcgacgtgac ggaaagggtt ttccctgcgg ccagggtacc atgattcgtg
     9061 agggcagcaa tcagtttcag tgtgacatca ccggtggcca ccacctgacc ctgaccactg
     9121 aagtcctgag cgtcaagcag caggttgcct gcactgtaca gccgccctgt accatgttgc
     9181 agcagtgaac tgcccttgac gccaagcccg gaggttccca gcagggtacc gctgttgctg
     9241 aatgtgtggt aattcaccag caggtccgca ccctgaagcg taccggtatt attcagcgtg
     9301 gttcctttaa cgtcggcact gccggtggca agtacgcgtc cgccgttgac agtattcacc
     9361 acatccagca gcagggtggc agcctgtacc agtccactgc cggtattcgc cagcacctgc
     9421 gccgtcagcg tgaggttact gccggagagg attttgccgt cgttctgcag acggtctgtg
     9481 gcgttcaggg aaaccccgcc accaccctgt atcgtgccct ggttactcag ggtcgcagta
     9541 ctgacattca gtgcattccg gctcatcaga acaccaccgg aacggttgtt cacgccaccg
     9601 gaggcggcca gcgtcagcgt ttcgccctgc agatgcccgc cgtttgtgag ttgtcctgcc
     9661 gtgatggtgg tggcatttcc ctgtaattgc ccgtcgtttg tgacactgtc tgccttcagc
     9721 gtcagcacac ctgcactgag cagttttccg ttcgcctgat tgttcagcgt ctgattcacc
     9781 gtgagcgtga gagtatccac accggtgatg tcacccgcac tggtcagtga gttcgccttc
     9841 agggtcagat tttttgcaat ccattgtccg ctgttgctta aattcagtgc actgagcgcc
     9901 atttcaccgt tcgaggtgac tttgctgccc gctgtgctga cgagctcacc cgtcagatgt
     9961 gcagtcaggc tgtcagccgc ctggatcgcc ccgctgtttg ccagactgtc tgcggtgatc
    10021 agcacccgtt tgccctgcca gtgtccggaa ctggtaatac tgcctgcggt gattgtcaga
    10081 tcgccgctgg tcagcaatga acctccgtta ttcatcagcg caggttgagg ggatgccata
    10141 cgggcggcaa gcgtcagcgc ggctatcccg gtgagcgtgc cactgttggt gacactgttc
    10201 tggcgaatcg tgacatggtt accctggaca gtgccgctgt tatccagtga gtttccatca
    10261 agggagagcg tgccggccga aagcagactg ccccggttgt ccatggtggc tgctttcagc
    10321 gtggtgtcac cctggctcat gatatcgccg gtactggtca actgaccggt tgccgaagca
    10381 gtaaggttac cggttgccag cacggaacca ctgttcgccc atctgtccgc ctgcacggtg
    10441 agattctgtc cctgcgtggt ccctgcggta tgcagtgttt taccccggag ggagaggtcg
    10501 cccgccgtca gccagcgtcc gttacggccc tgtgagaggg tgtcgcctgc aagcgccagt
    10561 gcaccggcgc cctgcaacag accatcacca tccagcgtgg tcgccctgac gttcagcgtg
    10621 tcagcgatga tttttcccgg attgctgagg gaggcagtgt ttaacgttaa accattatca
    10681 ccggtgataa gcccgctgtt gcggatgtcc ggtatatcca gcgtcaggtc tgcagcactg
    10741 tacagcgtgc cgttctgctg attatcaagc ctctgtgtgt taacggtgag tgaggcctcc
    10801 ccctgcaaca gaccactgtt ggtcagggtc tgtgactgtg tattcagggc ggaaccaaca
    10861 agcacaccgc tgctggtcag ttccggcgca ctgaggctga gcgacggggc gctgcttttc
    10921 ccgctgtggg tgagcttttc actggcgttc accgccatgg tctgttgtgc tgcctgcgta
    10981 cctgcaagac gtgcatctct ggcgttgatg ctgagatttt taccgctctg aagctgtgcg
    11041 cccgctgcgg tactcagttt gtctgcctga acccggaggg tgtcaccggc actgttttcc
    11101 ccgtccagcg ccactgttgt cacattcagc gtcatcgcag catcgctgtg ggtgaccgat
    11161 tttttaccgg agctcagcgc ctgcgcactg accgtcagcc ctttgccgcc ggacagcaca
    11221 ccgttctgtg tcacatcctg cgccttcagc accagtacat catcgctcac cagcgaacct
    11281 gtactggtca gtttcccact ggccgtgata tccactttgc ccttcgcgcc agtgcggccg
    11341 ctctgggtaa agtcgcgggt attcacggtc aggggaccgc cactgagcag ggagccactg
    11401 ttgctgagcg ttgtactgcc gagcgtcagg gaagccccct gaacagcacc actgttattc
    11461 agcgtgccgg catcgagtcc cgcatgacct ttcgccagca atattccgtc ctgtgtcagc
    11521 gtggtggcgc tggccgtgag attctgcccg gcggttatct gtccctgtgt tgtcagcgtg
    11581 tcactggcga cagtcacgat atcgcgggcc gcgttaatct ggctggcggt atcctgtgtg
    11641 atgtttttcg cggcaagcgt tacatcccgg ccggcagtca gtttttcatt ctgttgagtg
    11701 attctgccgc cggcggtcag gctgaggtcc ttgtcgctgt taagcgttcc attgctgaga
    11761 acgatatcgc tccggctgct gacgctcagg ttacctcccg ctttatggtc gccggttaag
    11821 gtgacgccct gaccttttgc agtgacggcc cccgtggcga gactgttgtt gacagtcagt
    11881 ctgccgctgg catccagggt gatatcgcca tcgcgggcat aaaggttacc aagattaacc
    11941 ccgacaccac tttcagtgga ggtcagatga atacgcctgg cgtacattcc accgagcgcg
    12001 ccggtatcaa cggcaacttt cggcacatca ccttcgccct tcagggcact gacgcgacca
    12061 tctgcagtta tccggttagc gcctgcagtg acagttaaat ccttcgcatg aagcgcggca
    12121 ttcacttccg ttgcacgggc aataatggat acggcatcgc tccgggtgcc gtccaggccc
    12181 gcgccattga tggtgatact gccttcagtc acctccagcg cctgcaggct gccgtcggca
    12241 ttcatcacag gtctgcctgt ggtgagcgtc gcgtgcggcg tgttgataaa accacagccg
    12301 tcacaggtga taccatacgg gttggcaacc atcacattcg ccgctttgcc ggccacttcc
    12361 gtatagccct gcagcagtga acggttaccg ccggtcactt cgttgatgat acccttcgct
    12421 tccccgcccg ctttcaggtt cgggttattc tgtatcagtc caccaagctg cgtcggatta
    12481 agcttaccgg tggcattatt gagaatcagc ccttccttcc cgacgttgta atccgtaaac
    12541 cggttatgcg aaatcccggc cccgttcggc gtggcaatgt tcacgaccgg cacaccattt
    12601 gccgctttat ccattccggc cccgttttgt ggggtgatga cggcccccac agccggtaac
    12661 aacggctgcc cggcgataat cgtactgata aggtaactca gcaggcggta agtgaagcga
    12721 acgggaggct gatgcatggt aataatccct taaaacgcga cggcaacgcg ccagtaaacc
    12781 gtgagatggt caggggcaag ccagtccggg taaacaagag gcagtccggc agtgaacgaa
    12841 ccggaaacat ggccactggt ggtgctgagc ccggcagcag caccccacag cgtgccggac
    12901 gagtacgggt catctctgtc agagtgcagc cagccaccgt ccagtgcagt cactgcacgg
    12961 acagttccca catatggcag ggagaacaga gaccaggaca gctcatttcg cagataacca
    13021 ccgttattac cggagatata ctgctcctta aagccacgca ctgaactctc gcccccgagg
    13081 ctcagttgtt ccacaccatg aagacggtcc ggtgaccact gggcataagc gctggtcagc
    13141 caccacaccc tgtccgtgac ggggcgctga aaactggcac tcaccgacca tttccggaac
    13201 tgatttacgg gcaggtctcc ccttttcccg tggtcgcttt ctgcgccgaa ccagggcatc
    13261 ccccgtgtga ataccggatt cagtgttccg acacccccca gaaacttgtg tgtgtgattc
    13321 agcccgacag aaaatgaagt gagtttacgg ctgctgccct gaagcagaac atcatccaga
    13381 taattgtgaa taatgcggtg ctgcagacct ccggtcagtg ctgtcttcat gttcccgtta
    13441 cggaacagga catgcgacag tcccagccgg tgagtctgca ggtctcccgt ggaacgccac
    13501 agccagcccc ggttatctat ggtgctgagg tagtcactcc atgaatacgt gtaatccacc
    13561 agggtatagc catacggcag actgacaccg gcggcaaaat tcctcgcatc atgtgacacc
    13621 gaaaagtcac tgctccgtcc cccgctgaca aaccagttgt cagccagccc cagaggatta
    13681 ttaaaggaaa ggacaccatt taactgccca gtaccggtat tcttctgccc gctgttgtcg
    13741 atgcccacgc tccctgtgac aggccattcc ggcaatgccg tcagtgtcac caccgaccat
    13801 ccctcacggt caccgggcga tatttcaatc tgtaccgact ccgtacgcag acgattaatc
    13861 tgctccatcc cctgctcaat atcccgcagg ttcagaacct ttccctccat tcccggaaaa
    13921 accatcttca gggtgcggcc aggaaggtca gcgccttccg cccggatttg ctgcagcctg
    13981 ccttccatga ccgttatgtg cagtacgccc cctgaaaggt cctgctctgt cagaaaggcc
    14041 cggctggtga tatatccccg gcgtatatag ccgtccgtca cggcatccgt gaccgcggtc
    14101 agccccgtga tattcagaca ctgattcacc cacggtgcca ccagtctgtc ggtttctgca
    14161 gacgtcagtc gggtggcccc actgacaaca atgcgtgaca ccgtaaagca gggaccttca
    14221 gtaccggcag acgtttccgg agacggcgtg atggtcagcg gcgcactgcg ctccagcgca
    14281 tcacgctgac gctggttttc atccagcaac tgctgctgtt gctgctgaat tgcactgcgg
    14341 tcacccggag acagcatggc tgccggagac gaaaatgcgg ggataaaaaa accagaaaaa
    14401 acggagaggg agagatagca tatccacgga gagctgcgcc ggaacatatc acaacagggg
    14461 acagacaacg cacgggataa aagggacgtt atcgtcagaa tgttgttctg ccggcgctgc
    14521 atgttctccc cgtattctgg cctgtgtgat gccagtgtaa attcatgtca aaaagtgtca
    14581 gcgagaaaat ttacacctga aaaagtctgt catgaaacag ttttatgaca agacatcaaa
    14641 aaaatgtcaa aaacgtgatc tttcttcagc ttttcagaat attgagtacc aggaagtcaa
    14701 tatcaattct gggacacata tgcatttacc attaaaaccg ggcacaacaa gctgttcagt
    14761 ctcaatccat accccgtgtt ttatcaatat cagaataaga atatctgcgg ataaataaaa
    14821 aaggaatgtc gagggagctt accgcagagg gctctccacc tcacaaacca tttacaaaaa
    14881 tgataccggc atatctttgg tccctgtcac aaccagacat tgacaatcca atataacccc
    14941 aaatat
//
LOCUS       DADTFG010000122        14678 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.123, whole genome shotgun sequence.
ACCESSION   DADTFG010000122 DADTFG010000000
VERSION     DADTFG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.123"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            29..322
                     /gene="hcp"
                     /locus_tag="J1138_003468"
     CDS             29..322
                     /gene="hcp"
                     /locus_tag="J1138_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HBA8455224.1"
                     /translation="MSDEIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGR"
     gene            473..1162
                     /locus_tag="J1138_003469"
     CDS             473..1162
                     /locus_tag="J1138_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA8455225.1"
                     /translation="MAFPDNYNRLFIPRQKTKDNNGLKTLNSKAVGRLLAAGGIYNGN
                     IEGFRETANKLGGDAPAGYDQVMDNKGLLIAGASIVAGVVLGRMRFPDLESFEHFGAR
                     GAVSGRPFNPDLAGGPIENLTIDGVTINREGIAIVEKHISRFDHDPANDVMISRLKKI
                     ANKELLPEKYDLNYYTHECREYQRYCNLGWETGEPNGLDGYELWNNAHTATLEDFKIK
                     DTDLFHPDAKK"
     gene            1159..1416
                     /locus_tag="J1138_003470"
     CDS             1159..1416
                     /locus_tag="J1138_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA8455226.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            complement(1905..4166)
                     /gene="metE"
                     /locus_tag="J1138_003471"
     CDS             complement(1905..4166)
                     /gene="metE"
                     /locus_tag="J1138_003471"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBA8455227.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4284..5237
                     /gene="metR"
                     /locus_tag="J1138_003472"
     CDS             4284..5237
                     /gene="metR"
                     /locus_tag="J1138_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBA8455228.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5125..6024)
                     /gene="bioP"
                     /locus_tag="J1138_003473"
     CDS             complement(5125..6024)
                     /gene="bioP"
                     /locus_tag="J1138_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBA8455229.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6100..6900)
                     /gene="yigL"
                     /locus_tag="J1138_003474"
     CDS             complement(6100..6900)
                     /gene="yigL"
                     /locus_tag="J1138_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBA8455230.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6908..7930)
                     /gene="pldB"
                     /locus_tag="J1138_003475"
     CDS             complement(6908..7930)
                     /gene="pldB"
                     /locus_tag="J1138_003475"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBA8455231.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8041..8661
                     /gene="rhtB"
                     /locus_tag="J1138_003476"
     CDS             8041..8661
                     /gene="rhtB"
                     /locus_tag="J1138_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBA8455232.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8722..9342)
                     /gene="rhtC"
                     /locus_tag="J1138_003477"
     CDS             complement(8722..9342)
                     /gene="rhtC"
                     /locus_tag="J1138_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBA8455233.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9406..11235)
                     /gene="recQ"
                     /locus_tag="J1138_003478"
     CDS             complement(9406..11235)
                     /gene="recQ"
                     /locus_tag="J1138_003478"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBA8455234.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     AVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11360..12229)
                     /gene="pldA"
                     /locus_tag="J1138_003479"
     CDS             complement(11360..12229)
                     /gene="pldA"
                     /locus_tag="J1138_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBA8455235.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12394..12861
                     /locus_tag="J1138_003480"
     CDS             12394..12861
                     /locus_tag="J1138_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBA8455236.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12913..13803
                     /gene="rarD"
                     /locus_tag="J1138_003481"
     CDS             12913..13803
                     /gene="rarD"
                     /locus_tag="J1138_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBA8455237.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            14113..>14678
                     /locus_tag="J1138_003482"
     CDS             14113..>14678
                     /locus_tag="J1138_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HBA8455238.1"
                     /translation="MNNILGSSINMSCTLFETITLFDDFSADDMQYGDMEEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFS
                     FFGQYKTLIQDLIDHFRYENGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDIS
                     STPTIGYRPLLLQKIKTELLSSRLNKFN"
BASE COUNT         3788 a         3740 c         3562 g         3588 t
ORIGIN      
        1 atcaagcaat aaagagagtt catcaaagat gagtgatgaa atttacctga ccataaccgg
       61 agaacaacaa ggatgtattt cctctcgttg cggaacgagc gcatcaatag gtaatcgctg
      121 gcagattggc catgaagatg aaatcttcgc gttctcgcta tctaatagta ttacgaatac
      181 aggcaaaggg tcacaattac atggccttag tttctgtaag ttgattgaca aaagctcccc
      241 gttacttatt aacgctatta acaataacga gcagttgttc atggagtttg acttctatcg
      301 gatcaataga tttggtcgat aggagaaata ttacaacatt caactccgcg gcgcgctctt
      361 atcagcaatc aatcacctgt ttactgagaa caatctggat acagaaacca tcactgtcag
      421 ttatgaatat atactctcca agcatctgat tgctaatact gaatttagtc atttggcatt
      481 tcccgataac tacaaccgtc tgtttatacc acggcaaaaa actaaagata ataatggcct
      541 caaaacactc aatagtaaag cggttggcag actgcttgct gctggcggca tatacaatgg
      601 gaatatcgaa ggattcaggg aaacagcaaa caagttggga ggagatgcac ctgctggtta
      661 cgatcaagtt atggataaca aagggttact catcgctggt gcttcaatag ttgctggagt
      721 tgttctggga agaatgagat tccctgattt ggaatcattt gagcatttcg gagccagagg
      781 agcagtatca ggaagaccat ttaatcccga ccttgccggt ggtccaatag aaaacctgac
      841 gatagatggc gtaacaatca atagggaagg tatcgctatt gttgaaaagc atatctccag
      901 atttgatcat gatccagcta atgatgtaat gatttcaagg ctaaaaaaaa tagcaaataa
      961 agaattgctt cctgaaaaat acgatctgaa ctactatact catgaatgcc gcgagtatca
     1021 gcgttattgt aatttaggat gggaaacagg tgaaccgaat ggtttagatg gatatgaatt
     1081 atggaataat gcacatactg caactctaga agactttaaa attaaagata ctgacttatt
     1141 tcatccggat gcaaaaaaat gacgattacg atagttgaat ttaacgtgct gaaagttatg
     1201 gctgagaaag atattgactg gagctggatg gtgctggatc gtactttagc tatacgaaat
     1261 attccaggat ttggcaacgt agcgaatatc gtaacaaagc tggttaatca tgggttggtt
     1321 gatatcgtaa atggtgaggg caactctaaa ccacgctaca gagtttctca atatggattg
     1381 aatcttatca aagaacaaca agacaacctc ttctgatcca gccattacag gagcatgatt
     1441 tacattgtga tctttcaata agatactcat caattttggt acagaatatc gtgatcacta
     1501 aaaatgaact taacgtgctg aacgttatga ctgagaaaga tataaactgg aactggatga
     1561 atctcgatcg cacactatcc ataaggcaaa taccaggatt cggtaatgtt gttaatattg
     1621 ttaacaaatt agctaacgaa ggtttggtta ttattgagga tagcgggcat aagtctatgc
     1681 ctcactacca cgttagtgaa aagggataca atctcgtgca acgtgagaat aaataacgta
     1741 gcgttgatta ctgaacggaa tcactcaaaa ctgtgttttg cgaaaaaaac gattcggtta
     1801 tttctactcc tgaagatctg cttcaatctg gcactcatgc aaaatggctt ccagtactgg
     1861 aagccattat cgtactgcaa tttacaatga ctcgttaata ctcatcatcc ccgacgcaaa
     1921 ttctgcgccg cctgcaccat gttcgccagt gccgcgcggg tttctggcca gccgcgcgtt
     1981 ttcaggccac agtccgggtt gacccacaga cgctctgccg gaatgcgttt tgccgctttc
     2041 ttcagcaagg cttcaatcca ttccacgctc ggtacgtttg gcgagtgaat gtcatagacg
     2101 ccaggaccga tttcatttgg ataatcaaac tcttcaaacg actccagcaa ctccatgtcg
     2161 gaacgcgagg tttcgatggt gatgacgtct gcgtccagcg ccgcaatcga atccatgatg
     2221 tcgttgaact cgcagtaaca catgtgagtg tggatttgtg tgtcatcttt cgccacggcg
     2281 gcgttgatac ggaaagcctc cacgccccac tggagatagg catcccagtc gctgcgacgc
     2341 agtggtaaac cttcgcgcaa tgccggttcg tcaatctgaa tgatgccaat tccagcggct
     2401 tccagatcgg ccacttcatc acgcagtgcc agcgcaatct gtttggcgat ggtttcacgg
     2461 ctgacatctt cacgcgggaa cgaccagcag agaatagtca ccgggccggt caacatccct
     2521 ttcaccggtt tatcagtcag ggattgcgca tattttgccc actccacggt aatcggtgcc
     2581 gggcggctaa cgtcaccaat aacaatcggt ggcttcacgc agcgggaacc gtagctctgt
     2641 acccaaccgt tttgcgtaaa gacaaagcca tccagatgct cgccaaagta ttccaccatg
     2701 tcattacgct cggcctcgcc atgtaccagc acatccagtc ccaaacgttc ctgctcaaca
     2761 atggcctgct tgatatgttc cgcaatgccc gtgcggtagt tattggcgtc gagattaccc
     2821 tttttaaaat ccagacgcag ggtacgaatc tccgtggttt gcgggaagga accaatcgtg
     2881 gtggtcggcc acgcgggcag tttaaaacgc gcacgctgag cttcagcacg cacttcatag
     2941 acattcgcac gctgactgtc ctgggcggtg atcgccgcca gacgcttttc tactgccgga
     3001 ttatgtacac gagtagagtg acgacgcgcc tgaatcggag cgctccactc tgccagagct
     3061 gccgtatcac cactgttcaa cgcatcgcgc agcaatgcca gttcatgaca tttttgcagg
     3121 gcgaaggcaa accagctttt cacttctgca tcaagacgcg tttccacgct caggtcgatg
     3181 gggctgtgca gcaacgagca ggaagatgcc acccacaaat cgcgtttgcc gacaatgtcc
     3241 ttaatttgcg catatttctc ggtaagatcg gcgcgccaga cgttacgacc attgataaga
     3301 cccgcagaca gcagccagtc agaaggcaga cgcttgtgca gttcagcaac gtcatcttta
     3361 ccatgtacga gatcgacatg cagaccctga acaggcagcg cagtaatcgt gtcgaggttt
     3421 ggcgttacgc cttcaaaata ggtagtcagc agcagtttca cctgcccctg cagagcatcg
     3481 taagcgggtt tgtacgcatc cagccacgcc tgcggcagtt ccagaaccag cgcgggttca
     3541 tcaatctgta cccactcgat gccgcgtttt gccagttctg ccagcacttg ctgataaacc
     3601 ggcagaatgt cgttcagcag gctcaggcga tcaaactgtt cacctttcac tttacccagc
     3661 cacaggtagg taaccggccc cagcagcacg ggcttgacct tgtggcccag cgccagcgcc
     3721 tcgtccactt cctccagcag ctgcgtccag gtcagtttga actgttggcc tttaacgaac
     3781 tccggcacca tgtagtgata gttggtgtta aaccatttgg tcatttccgc tgccgccgca
     3841 ggttcgccag tcggtgcacg tccacgacca atacggaaca gggtgtcgat atctaccgaa
     3901 ccatctttgt tctggtgacg cgccggaacg ttacccagca gcaggctggt ggtcaggaca
     3961 tgatcgtacc aggcaaaatc acccaccggc agcaggtcga tacctgcttg cttttgttga
     4021 tcccagtgac gagcacgcag ttcacgccct accgccagca gttcttcacg cgtggagttc
     4081 cccgcccaat aactctcttg cgcttttttc agctcgcgac gcaggccaac gcgagggaaa
     4141 ccgagggtgt gattaagaat tgtcattata tgccccttat ggaattttta gtatttagcc
     4201 gtccagatgt ttacacatcc ataattggcg gttactgtat attcctcaag cgcaaattat
     4261 tcatgccgaa gtgaaggact ttcatgatcg aagtaaaaca cctgaaaacg ctacaagcgt
     4321 tgcggaactg cggctcactc gcagccgctg cggcgacgtt gcatcaaacg caatccgccc
     4381 tgtctcacca gtttagcgat ctggaacaac gccttggctt ccggctattt gtgcgtaaga
     4441 gccagccgct acgctttaca ccgcagggag aaatcctgtt gcaactggca aaccaggtac
     4501 tgccgcaaat tagccaggca ctgcaagcct gcaatgaacc gcagcagacg cgtctgcgca
     4561 ttgccattga gtgccatagc tgtattcagt ggctgacgcc cgcgttagaa aatttccata
     4621 agaactggcc gcaggtggag atggatttta aatcaggcgt aacgtttgac ccgcagcctg
     4681 cattgcagca gggcgagctg gatctggtga tgacctccga tattctgccg cgcagtggcc
     4741 tgcattattc gccgatgttc gattatgaag tgcgtctggt gttggcacct gaccatccac
     4801 tggcggcgaa aacgcgtatt acaccggaag atctcgccag cgaaacgttg ttaatttatc
     4861 cggtgcagcg tagccgactg gatgtctggc gccattttct tcagccagca ggcgttagcc
     4921 cgtcgctgaa aagcgtcgat aacaccttgt tgttgattca gatggttgcc gcgcggatgg
     4981 gtattgccgc cctgccgcac tgggtggtgg agagttttga gcgccagggt ctggtcgtaa
     5041 ccaaaaccct gggcgaagga ttgtggagcc gactgtacgc cgccgtgcgc gatggcgagc
     5101 agcgtcagcc gattacagaa gcgtttattc gctcagcgcg caatcacgcc tgcgatcatc
     5161 tgccgtttgt gaagagcgcg gagcgaccca cttacgatgc acccacagtg aggccaggat
     5221 caccagcgcg cctgtaataa acgttggcca gtgcggctgt tggtgccaga tagccaggtt
     5281 aaccaacagc cctgccggaa cgtgcatatt attcataatg cccagcgttc cggcgtccac
     5341 ctgcgtcgcg ccgtagttcc acataaagta gccaataccg gaagccacca cgccaagaaa
     5401 caccagaatg ccccattgca gcgtggtttg cggcattttc tgcgcatttc ccagcaagaa
     5461 ccatgcaatc actgccacca ggaacgcgcc aagatagaac cacgcaaagg cgttatgctg
     5521 cggcatcggg cgagtttcca tcagacgttt gtaacccacc atgccgatgg caaaagtgat
     5581 attggagagc tgcaccagca acaagccagt ccagaaatgg tcggtgacct gatcatagcg
     5641 aataatcccg gcaccaatca ccgccaacaa ggcgctaaaa gcatagcccc agcgcagacg
     5701 gcgcttactc atgatgtcat aaatcagcgt gatgtagagc ggcgtcagta cggtgaacag
     5761 caacagttcg gagaccgtca ggtagagata ggcgcggaaa ctcagcatat acatcacacc
     5821 gagctgcatc gcgcccacca gcatatacag gccaaccgtt ttcaggctat tgccacgggt
     5881 gcgcagaaac ggcagaaaaa cgagtgccgc cagaccaacg cgcaccagca ccgcaaaata
     5941 gctatcgacg tgccccgcaa ggtactcgcc ataaaagcta aaggagaagg cccacagaat
     6001 cgtggtgatg ataagtagcg ccacaatgga aaactctttg attaacgagt atccattgta
     6061 gcgaagtatc aggttgacaa ctgaccaaat aaagaacgat tacgataaat agagcttacg
     6121 cagataatgc ggcactgcgt cctcggcgtt agtaccaatc acttccagct ctggatgcag
     6181 atctttcagc cgttgatgcg cgctgcccat aatgcagcct ttccccgcca tcgacagcat
     6241 ttcggcatcg ttcattccat cgccaaaggc aatacaatct tgcaggctgt agcccagttt
     6301 cttcgctacc gcttccaggg catggccttt tgaaacgccg ccagccatga cttccaggca
     6361 ggttaaggta gagaaactga cattgacgcg atcgccccag cgagcgttaa tcgcttgctc
     6421 cagtggcaga agtttttcat gggaatcgca ggtgaagaac actttgctga cgccttccgg
     6481 ctccagcaat cccggctcat acagcgcata tttgaacacc gcttctttaa aaaagcgcat
     6541 ctcttccggg cgatggcgat tcataaacca ttcgtcgtcg cgataaacgt tagtaatgat
     6601 gtccggattg tcgttgacta cgccaaacag atcactggca atgtcgcgat ccaggttatg
     6661 agcaaaaatc aggttgccat ccagatcgtg aacgcgcgcg ccattggagg taatcatgta
     6721 agacttaatc tccagattat cgcgaatttg ccccacatca acgtggtgac gaccggtcgc
     6781 aaacacaaag ttgatgccgc gcgcggtgag cagcttcaga gtttctttgg cgtaagggga
     6841 taacgtatgg tcgggagaaa gtaacgtgcc atctaaatca gacgcaacaa cctggtacat
     6901 aagaaattta aacctctgta gagcggtttc cgctgggtga gttatgcctg ttgaaaaaat
     6961 caacgatagc gtggagcgcg actgagcgca ttgcgtcctt ttcaaaaagg atctcatggt
     7021 acgcaccttt aattaccaac ggccgtcctc cttcgacagg atggcccgca gcggtgcgta
     7081 gttcacaaaa acggtcgtgc atgcggttat ccaccacgcg ttcctcttca gcttgtaaga
     7141 gaagcgttgg cgtggcgtca tcacccgcac cggctaacac ctgttcgcca gccagaatac
     7201 tttcgcgtac ccaatggtag gtcggcccac cgacgcgaat cgttgggtca tcagcataga
     7261 aacgtaagtt acgtcgatat cgctgtctgc tgtgggtcag tacgttgata gcaaacggca
     7321 acgcgcgcca gcgcccggtg cctatcgcat agccatcacg gaaacgtgga tgcgcttcgg
     7381 cccagttaag gatctgccgt gccataaatg acggcatacg aatcacaatc ccaaacattg
     7441 gcgcagttag cgcaatggcg tcacatacac ctggatggcg ttgcagaaat aatgtggaga
     7501 tcgcaccgcc catcgaatgt gccagtatat agcgtttacg ccacggaccg ggctgaacct
     7561 cctgttgcca gaatgccgcc agatcatcaa cataatcatt aaagcgatta acatgcccga
     7621 gatgtggatc ggctaacagg cgaccggaac gtccctgccc gcgatggtcg atgattaaaa
     7681 catcaaaccc caaatggaac aggtcgtagg ccagttccgc atattttacg tagctctcaa
     7741 tacgccccgg acagatgact accacccggt catggtgctg tgcgcgaaaa cggacaaagc
     7801 gaaccggaat gtcatccaca ccagtaaact ctgcttcatc acgctgacgc cagaaatcag
     7861 tcagcggtcc catggtaaaa gcagcaaacg cgttttctct tgtttcccag tctttttgct
     7921 gctgaaacat cgggtaatct gcctcttaaa ccacgtaaaa tcgttttttt aagcgtgcct
     7981 gacacaacgc tgcgacagta gcgtattgtg gcacaaaaat agacacaccg ggagttcatc
     8041 atgaccttag aatggtggtt tgcctacctg ctgacatcga tcattttaag cctgtcgcca
     8101 ggctctggcg caatcaacac catgaccacc tcgctcaacc acggttatcg cggcgcggtg
     8161 gcgtctattg ctgggcttca gaccggactg gcgattcata ttgtgctggt tggcgtgggg
     8221 ttggggacgc tattttcccg ctcagtgatt gcgtttgaag tgttgaagtg ggcaggcgca
     8281 gcttacttga tttggctggg aatacagcaa tggcgcgccg ctggcgcaat tgatcttaaa
     8341 tcgctggcct ctactcaatc gcgccgacat ttgttccagc gcgcagtttt tgtgaatctc
     8401 accaatccca aaagtattgt gtttctggcg gcgctatttc cgcaattcat catgccgcag
     8461 cagccacaac tgatgcagta tatcgtgctc ggcgtcacca ctattgtggt cgatattatt
     8521 gtgatgatcg gttacgccac ccttgctcaa cggattgctc tatggattaa aggaccaaag
     8581 cagatgaagg cgctgaataa gattttcggc tcgttattta tgctggtggg agcgctgtta
     8641 gcatcggcga ggcatgcgtg aaaataatgt cggatgcggc gtaaacgcct tatccgactt
     8701 actctgatga cgcgtctggc attaacgcga aataatcaaa tgaatgccaa atccggcaaa
     8761 taacgccccg gcaaaaccat caatccactt cgccagacgt tgataaccac ggcgcatttg
     8821 cggcagagca aacaggctgg caacgacggt aaaccacgcc agtgtttcga caataatcaa
     8881 cgcgaaaatg ccccagcgtt cggtggtgcc gacattatca ccgacaaaca gcgagaacac
     8941 cgaaccaaag tagataatcg ctttagggtt cgcgagattg gtcagtaagc ccttaagaaa
     9001 actgcgcccg cttttcgcca gttcgacttg tggcgcaggg gcagaaacaa cctctttttt
     9061 cagtgcaccg cgtagcatct ggtaacccat ccagcagaga tacaggccac cgcccaccat
     9121 aatcagcgta tgcagccagg ccattttttc gataatcaaa tgtaggccaa gcaacgcaat
     9181 cccagcccaa accattacgc cgcaggtaat gcccagcacg cccatcatcg cttctttacg
     9241 ggaacgacta acagcggtct gagagacaaa aaagaaatcg ggaccggggc tcataagcgc
     9301 cacaatgtgc accatggcga cggtgaggaa taacatcaac atacattaga ctcgcggggg
     9361 aaacgtttac ggagtcttca tcctggcact tttttatgct gctgactact cttcgtcgtc
     9421 accatcgaca tgcgcacgaa tcagcgccat aaacggtttg ccaaagcgtt ccagcttgcg
     9481 catcccaacg ccgttaacgc tgagcatttc gctggcggtg atcggcatct gctccgccat
     9541 ctcaatcaag gttgcgtcgt taaacaccac gtacggcggg acattgcttt catcggcaat
     9601 cgatttacgc agtttgcgta atttggcgaa cagtttgcga tcatagttgc cgccgaacga
     9661 tttctgcatc gctttcggtt tgagcgccac gatacgcggc acggcaagtt gcaaagagga
     9721 ttcgccgcgc agcaccggac gcgcagcctc tgtcagttgt agggcagaat gctgggcaat
     9781 attttgcgtc accaggccga ggtgaatcag ctggcggatc acgctcaccc aatgttcatg
     9841 gcttttatca cggcccatgc catagacttt cagtttgtca tgaccatagt cgcggatacg
     9901 ctgattatta gcaccgcgaa tcacttccac cacataaccc atcccaaacc gctgattcac
     9961 acgaccaatg gtggaaaggg caatctgcgc atcggttgaa ccatcgtact gttttggcgg
    10021 atcgaggcag atatcgcagt tcccgcacgg ctcctgacgc ccttcgccaa aatagttcag
    10081 caataccaaa cgacgacaag tttgcgcttc ggcaaacgcg cccatcgcat tgagtttatg
    10141 acgttcgata tcctgcaact gcccctgcgg cttctcttcc agacaacggc gcagccacgc
    10201 catatcagcc ggatcgtaaa acagcatcgc ttccgcgggc aggccatcac gcccggcgcg
    10261 tccagtttcc tgataatagg attcgatatt gcgcggaata tcaaaatgca ccacgaagcg
    10321 aacgttaggc ttgttgatgc ccatgccgaa cgccaccgtc gccaccacaa tttgcaggtc
    10381 atcgcgctgg aatttttcct gcacatcagc gcgaacatta ttttccagcc cggcatgata
    10441 ggccgccgcg ctaattccct tgctttgcag gcgcgcagcg gtgtcttcta ctttcgcacg
    10501 actattacag taaataatcc ccgacttacc gcgttgttcc tgtacataac gcatcaactg
    10561 atcgagcggt ttgaatttct ccatcagcat gtagcgaata ttcggacggt caaaactgct
    10621 gatttgaatt agcggatcgt tcagccccag caggcgcacg atatcctggc gcgtggtgtc
    10681 gtcggcggtg gcagtcagcg ccataaacgg cagcgtcgga aaacgctgac gcagctgacc
    10741 gagggcagcg tattccgggc ggaaatcgtg gccccattgg gagatacagt gcgcttcatc
    10801 aacggctaat aacaccggat tccagtgcgc cagatgctca agaaagttat ccagcatcag
    10861 gcgttccggc gcgatataaa gcaggcggat ttgcccggtg cgacagcctg tcataacttc
    10921 aagttgctgc tcgcgggttt gcgtcgagtt aaggcacgcc gccgccacgc cgttggcttg
    10981 cagttgatcc acctgatctt tcatcaagga aatcagcggt gaaacaacca cggtaaggcc
    11041 gtttagcaat aaggcaggga tttgatagca aagggatttt ccgccaccag tgggcatgac
    11101 ggccaggcaa tcgcgaccgg aaagtacggt gtcgataatc tcttcctggc cggggcgaaa
    11161 ctgttggtag ccaaaggttt cttgtaaaac ctgtttagct ccggactcca gattcaacac
    11221 ttccgcctgc gccacattca ccccgattgc ctgaaataaa aacaggcgct attttcagcg
    11281 cctgcgcgag aaactgcaat gcttttagtc tctgagaggg taatcctgga tgctggattc
    11341 cagatttgcc aaccattcat caaaacaggt cgtttagcat aactcccaca ccaacgcggg
    11401 tctggttgaa gttatagtcg atgagcgatt cgccgtaacc gctgtaaacc tgagtataaa
    11461 ggcgcacatg tttggtgatc gggtagctta agcctaactc cgcgccgccg taaccggtgt
    11521 tccagttgta ctgccctttc gcgctcagta ccgcatcacc gaggtgatag ccgattttaa
    11581 gctggtagta acccatatat ttggtgatgt ccgggttatc gtccgtattc cccaccacat
    11641 accacggctt cacctctacc agccagttac cgttttctgc catcaggcgg gtataaaggc
    11701 ggttccagct gcgggaagtc gggtctgaac gcccgtttga gtcgtggttg tagcccattt
    11761 ccacatcgcg cagcgtccag ccagcaaagt tgtaatccgt ggcaaaaccg aggaacaatt
    11821 gcggttcgta gttagtttca cgaaacggtg atgactcatc gctattagaa agctgccacc
    11881 aggatttctg tgtgtaggag gcgcccagta cagagtttgg ccccaaaatc ccacgccaca
    11941 gcggaaacgc caggctcaac tggaacttta cttcatcctt acgcgcgttt tccgcccagt
    12001 cgtaactggc aatcgcttct ttattcagat cgctggtttg ggtgtagatg atgtagttgg
    12061 tgtcataagg ataaagggtg aacggattgt catgctcctg cagcatattg gcgataatac
    12121 tgccacgcac cgctggcgcg tcatgcacct ctttcaccgt tgcctcttgt gcatatactg
    12181 ccataggcaa cataaacacc ggcaacaacc agccctgcag agtccgcatc gctcgtgttc
    12241 tccgtaacga attattgata aggtgaatat ttttctgcgg gctattttac atacttaact
    12301 ccatagccgt tagttatccc ttcctaaagt agaaaccaac aacaaagaag cataaaatca
    12361 acatttcatt aaccaatgaa gtgtaaggat gttatgtctg ccgtactgac cgctgaacaa
    12421 gccctcaaat tagtgggtga gatgtttgtt tatcacatgc catttaaccg cgcattgggg
    12481 atggaactgg agcgttacga aaaagagttc gcacagctgg cctttaaaaa tcagccgatg
    12541 atggtgggca actgggcgca aagcatttta cacggcgggg tcattgcgtc ggcgctggat
    12601 gtcgccgcag gtctggtgtg cgtgggaagt accttaaccc gccacgaaac catcagtgaa
    12661 gatgaactac gccagcggct atcgcggatg gggaccattg atcttcgcgt cgattatctg
    12721 cgcccaggca gaggcgagcg ttttactgct accagtagcc tgttacgtgc aggcaataaa
    12781 gtcgccgtcg cccgcgttga attacacaat gaagaacagc tttatattgc cagtgccacc
    12841 gccacctata tggtaggttg aagacataaa agcaggtaaa ctgctgttac caattaaaaa
    12901 tgagtattcc cgatggatgc aaaacaaacg cggcagggcg tattactcgc tcttgccgct
    12961 tattttattt ggggtattgc gccagcgtac ttcaaactga tttactacgt gcccgccgat
    13021 gagatcctga cgcatcgcgt gatctggtcg tttttcttta tggtggtgct gatgagcatt
    13081 tgccgccagt ggtcctattt aaaaacgctg attcagacgc cacagaaaat ttttatgctg
    13141 gcagtctctg ccgtgctgat tggcggcaac tggctactgt ttatctgggc ggtgaacaat
    13201 caccatatgc tggaagcgag ccttggttac tttattaacc cgctggtgaa cattgtgctg
    13261 gggatgattt tcctcggcga gcgattccgc cggatgcaat ggctggcggt gattctggcg
    13321 atatgtggag tgctggttca gttgtggact tttggttcac tgccgatcat cgcgctgggg
    13381 ctggcgttta gttttgcttt ctacggcctg gtgcgcaaga agattgctgt tgaagcgcaa
    13441 accggcatgt taatcgaaac tatgtggttg ctgcccgtgg cggcgattta cctgtttgct
    13501 attgccgaca gctcaaccag tcatatgggg caaaacccga tgtcgctgaa tttactgctg
    13561 atcgccgccg gtattgtcac caccgtgcca ctgttgtgtt ttactgcagc ggcgacacgc
    13621 ctgcgtctct caacgttagg ctttttccag tacattggtc cgacgctgat gttcctgctg
    13681 gctgtgacgt tttatggtga aaaaccgggt gccgataaga tggtaacttt cacctttatt
    13741 tgggtggcgc tggcaatttt tgtgatggat gcgatttata cgcagcgtag aacgtccaaa
    13801 taaaaaataa atatattctg aaaaaatatg ccgactcttg catgagagtg ggcatattgc
    13861 catgctgaca ggagactcac ataaggaaag gcagaatttc cagacgtcgt tgtatatttt
    13921 catttgcttt aataaatttc atttttggta aattcagcat tatgttatta gcgatgagca
    13981 agaccaatca ttaaccaagt tatcttcaga atgtaataaa aaatgtgaag tgcatttctt
    14041 ttttaaaatt ttttacccta ttatttctta tcaaattaac attgccctta attataagaa
    14101 aggagaatat aaatgaacaa catattaggc tcatcaatta atatgtcctg tacacttttc
    14161 gaaactatta ctttattcga tgatttttct gctgatgata tgcagtatgg ggatatggaa
    14221 gaacaagatt ttctctctct cggcttaagc gacatctctg caaaagttga tccttaccgc
    14281 ctcattaaat accattttcc tgggccgaat tctacttatg gtgcttttag tgttccaact
    14341 tctggaacaa aaatatcaca aagtgaatgt attgatattt tatttgcaga aatgaaagat
    14401 ctggcaaaaa tgttttcctt ttttggacaa tacaaaacac tcatccagga tctgattgat
    14461 cattttagat atgaaaatgg caacagtttc catagccagg aattgaactt atcttttcat
    14521 gaacgaataa acaaatatga ttataatagc ccaatacgtg taatcaaaga atgtatcgaa
    14581 aatgatattt cttcaacacc tacgatagga taccggcctc ttctcctaca aaagattaag
    14641 accgaattac tttcatcaag actaaataaa ttcaatga
//
LOCUS       DADTFG010000123        14629 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.124, whole genome shotgun sequence.
ACCESSION   DADTFG010000123 DADTFG010000000
VERSION     DADTFG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.124"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(379..2043)
                     /gene="asnB"
                     /locus_tag="J1138_003483"
     CDS             complement(379..2043)
                     /gene="asnB"
                     /locus_tag="J1138_003483"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBA8455239.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2299..3051)
                     /gene="nagD"
                     /locus_tag="J1138_003484"
     CDS             complement(2299..3051)
                     /gene="nagD"
                     /locus_tag="J1138_003484"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBA8455240.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(3099..4319)
                     /gene="nagC"
                     /locus_tag="J1138_003485"
                     /gene_synonym="nagR"
     CDS             complement(3099..4319)
                     /gene="nagC"
                     /locus_tag="J1138_003485"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBA8455241.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            complement(4328..5476)
                     /gene="nagA"
                     /locus_tag="J1138_003486"
     CDS             complement(4328..5476)
                     /gene="nagA"
                     /locus_tag="J1138_003486"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBA8455242.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAISVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            complement(5536..6336)
                     /gene="nagB"
                     /locus_tag="J1138_003487"
     CDS             complement(5536..6336)
                     /gene="nagB"
                     /locus_tag="J1138_003487"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBA8455243.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            6669..8615
                     /gene="nagE"
                     /locus_tag="J1138_003488"
     CDS             6669..8615
                     /gene="nagE"
                     /locus_tag="J1138_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBA8455244.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            complement(8704..9984)
                     /locus_tag="J1138_003489"
     CDS             complement(8704..9984)
                     /locus_tag="J1138_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane transport protein"
                     /protein_id="HBA8455245.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(9998..10468)
                     /locus_tag="J1138_003490"
     CDS             complement(9998..10468)
                     /locus_tag="J1138_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBA8455246.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            complement(10465..11634)
                     /locus_tag="J1138_003491"
     CDS             complement(10465..11634)
                     /locus_tag="J1138_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBA8455247.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHNGTLVALF
                     RSRWADNIYISHSADNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     EHRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            complement(11670..12428)
                     /locus_tag="J1138_003492"
     CDS             complement(11670..12428)
                     /locus_tag="J1138_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA8455248.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLTETHESHSH"
     gene            complement(12488..13375)
                     /locus_tag="J1138_003493"
     CDS             complement(12488..13375)
                     /locus_tag="J1138_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBA8455249.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADALSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSAGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANIAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            complement(13379..14536)
                     /locus_tag="J1138_003494"
     CDS             complement(13379..14536)
                     /locus_tag="J1138_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBA8455250.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDAPGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     AALGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
BASE COUNT         3563 a         3764 c         3761 g         3541 t
ORIGIN      
        1 cttttttttc gcgatacctt atcggcgttg cggggcgcat tatgcgtata gagccttgca
       61 gcgtcaacct ctttttcaag gaaaattgct cgaaagtgac tgtttggtta ggttgcgaac
      121 agcatggcgc tatattcgtc aattattgtt tactttgtgt tttttcccca ccctacagcc
      181 attcttttgt catacaggat gaaattcgga attcaacaat agtggtggga aattaatcta
      241 tgaaatactg gcctacagta atgagttgtc aaacatcgat gtggcaaacc cggaacattt
      301 ccttactgca tatcagaatc aacaagcgcc ccagtaactg aaattgcccc ggatttctcc
      361 ggggcaatat tacacgtctt atttatatgc cgactggtga acgccaaccg cgcgaccaga
      421 cggatcgtcc attttcttga acgcttcatc ccactcaatc gctttagcgg aagaacaagc
      481 gacggaagga ccgcccggca cgcactcagc ggcgctcgga agcgggaaca attcttcaaa
      541 gatctcccgg tacagatacg cttcttttga ggtcggcgtg ttgtacggga agcggaagcg
      601 ggcagtttcc agctgttgat cagaaacctg ctgcgctgcc acttctttca gggtgtcgat
      661 ccaactgtaa ccgacgccat cggagaactg ctctttttgc cgccaggcca cgcttgcagg
      721 cagatacgct tcaaaacatt cacgcaggat gtgtttttcc attttgccgt taccgcacat
      781 tttatcctgt gggttaatgc gcatcgccac atcaaggaat tttttgtcga ggaacggaac
      841 gcgtgcttcc acgccccagg ctgacatcgc tttgttggca cgcgcgcagt catacatatg
      901 cagggccagc agtttacgca ccgtctcttc atgcaactct ttagcgttcg gcgctttgtg
      961 gaagtataga taaccgccaa acacttcgtc agaaccttca ccggacagca ccattttaat
     1021 gcccatcgcc ttgatcttac gcgacattaa atacatcggt gttgaagcgc gaatagtggt
     1081 cacatcataa gtttcgatgt ggtaaatcac gtcgcggatg gcatccagac cttcctgtac
     1141 agtgaagtga atttcgtgat gcaccgtgcc cagatggttt gccacttcct gggctgcttt
     1201 cagatccggt gagcccggca gacctacagc aaaggagtgc aactgcggcc accaggcttc
     1261 agagcgttcc tgatcttcca cgcgacgggc ggcgtatttc ttggtgatag cggaaataat
     1321 tgaggaatcc agaccaccag aaagcagcac accgtaaggc acatcagaca tcagatggct
     1381 tttaacggaa tcttccagtg cctgacgcag ctcgtttttg tcggtcacgt tatctttcac
     1441 cgcatcgtag tcgaaccagt cgcgatgata ataagaacgg atttcgccgt cctgactcca
     1501 caaatagctc cccgccggaa actctttaat cgtgcggcaa actggcacga gggctttcat
     1561 ttctgaggcc acatacagct gaccgtgttc gtcatagccc atatacagtg ggatgatccc
     1621 cagatggtcg cgaccaatca gatatgcatc tttttcgctg tcgtacaggg caaaggcaaa
     1681 catgccctgc aagtcgtcga gaaattccgg ccctttttcc tgatacagcg cgaggatcac
     1741 ttcacagtca gacccggtct ggaactggta acgatcgcca tattcagcgc gcaatgcctg
     1801 gtggttgtag atttcaccgt ttactgccag cacatgagtt ttttgttggt tgtagagagg
     1861 ttgcgccccc gcgttaacgt caacaattga cagacgttcg tgggcgagga tggcgttatc
     1921 gctggcataa ataccggacc agtccgggcc acgatgacgc atcaggcgtg acagctcgag
     1981 tgctttctta cgcagctcaa ctgcgtctgt tttgatatcg aatacgccaa aaattgaaca
     2041 cataaccttc tccgttaacc tggtgtttac tgcgtgttgt gtttacttgt taaaaaaatg
     2101 ccgcaaagca gcacggtgcg caagcgattt ggcagtggaa aaataaaaaa cgtaatggcg
     2161 attgctgatt gatgaaaaag gggagggctt ttgggtatat ttattgatgg atcgataatt
     2221 tttgacgggt tttattgaat ggggtgattt taatgatggg taaaaataag aaaccacgcc
     2281 ctgaggcgtg gttgctattc agataacgtc gatttcagcg accgatgggt aaatccaact
     2341 tgggcggaac ggcatactgt cgatatcgtc gagtgacgaa acaccagaaa gcaccagaat
     2401 cgtctccaga ccggcctgga agcccgccag aatatcggta cgcaagttat cgccgacaat
     2461 caccgtttct tccgaatgcg cctgcatttt gtttaatgcc gcgcggatga tccacggact
     2521 cggctttcca acatagaacg gtttgcgccc ggagattttc tcgatccctg cacacaacgc
     2581 gccacaggcg ggataaaaac cgcgcccgtg ggtgtccggg ttggtggcga taaagcgtgc
     2641 accgttagcg acgaaatagg ctgctttatg catcatgtcc cagttgtagg aacgcgtttc
     2701 gccaacaatc acaaaatcag ggttcacatc ggtaatagtg aaaccggctt tgtacagttc
     2761 atgaatcagt gcgccttcgc ccaccacata cgctttcttg ccttcctggc gacgcaggaa
     2821 atcggcggtc gccatcgcgg aggtataaaa cacgctatcc ggtacatcga caccagcggt
     2881 ggcaaagcgg ttcgccagat cttgcccggt ctgcgaagga tagttggtca gcaacaccag
     2941 cggcaggcct ttatccataa tcccgtgcaa aaattccgct gcacccggta cggcgacgtt
     3001 atcgtgcatc agcacgccgt cgatatcgca aattacattt ttaatggtca tggactaccc
     3061 agaatattga caacaataag cgccactata aaagcacatt aattttccag caaatgctgg
     3121 agcaaaatac cgttgagcat ggcgcgtttt accagcgcaa aagcgccgat tgccgagcgg
     3181 tgatccaact cagaacgtac caccggtaga ttagtacgaa acgccttcag cgcctgggta
     3241 ttaatgcagc tttcaatagc agggagcagc actttatcgg cttcggtgat ttctccagca
     3301 ataacaattt tttgcggatt aaataagttg atagcaatgg cgatggtttt acccagatga
     3361 cgaccgacat attcaatcac ttccgaggcc aggctatcgc ctttgttcgc ggctttgcag
     3421 atagttttga tggtgcagtc gtccagtggc acacggctct ggtagccctg ctttaacaga
     3481 ttcaacaccc gttgttcaat ggcagcattg gcggcgatag tttccagaca gccaaagttg
     3541 ccgcagtggc agcgttcacc cagcggttcg acctgaatat ggccaatttc accgacgttg
     3601 ccgttgcgcc cgataaaaat tcgcccgtta gagataatcc cggccccggt tccgcgatgg
     3661 acacgcacca gaatggagtc ttcgcaatcc tgacttgcac cgaagtagtg ctctgccagc
     3721 gccagactgc ggatatcgtg gccgacaaaa caggtcactt taaaacgctc ttccagtgct
     3781 tcaaccagcc cccagttttc tacctgaata tgcggcatgt aatgaatttt gccgctgtcc
     3841 gggtctacaa gccctggcag gatcaccgaa atcgcgatca gctcgcgtag cttgcgctgg
     3901 tagctatcaa taaactgagc aatggcattc agcagggcat gttccagcgt ctgctgggta
     3961 cgttccggca gcgggtaatg ttcttctgcc agcactttgc tgctgagatc aaacaaggtg
     4021 atggtggcgt cgtgacgacc aagccgtacg ccgattgcgt ggaaattgcg ggtttcggtg
     4081 acgatggaga tagcgcggcg gcccccggtg gaggcctgct gatcaacttc tttgatcagc
     4141 ccgcgttcga taagctgacg cgtaattttg gttacgctgg cgggggcaag ctgactttgc
     4201 tcggcaatct gaatccgcga gattggcccg tactggtcaa tcaggcgata aaccgccgcg
     4261 ctgttaagct gttttacgag atcaacatta cctatctgag cttgtccgcc tggtgtcata
     4321 ctttctctta ttgagttacg acctcgttac cgttaacgat ggtcctggtg attttaaaat
     4381 caggtgtgaa tgcagtcagg ttggctactt tacctgcggc gagcgtgccg agacgtttct
     4441 caacgctaat cgcacgtgcc ggatagagcg tcgccatacg cagcacttca tccagtgcga
     4501 taccacaatg ttcgaccaga ttacgcacgc cttcaatcat ggttaaggat gaaccgctta
     4561 acgtaccgtt ctcatccaca caaagtccat tacggtagta tattgtttta cccgcaaaaa
     4621 tgaactgttc aatgttggca cctgctggcg cggtggcatc ggtaaccaga cacaatttgt
     4681 cgcctttcag acgtttagcg ttacgaatgt tggcgtaatc aacatgcagg ccatcagcga
     4741 taataccgca ataaatgtca gcttcgtcga ggatcgcgcc cgccaggccc ggttcacgac
     4801 cggtaatata cggcatcgcg ttgtacagat gggtggcaaa ggtaatcccc gcgcggaaac
     4861 cggctttcgc ttctttcaac gtcgcgttgg agtgaccagc agaaaccaca atcccggcat
     4921 ttgccagttt gctgatgact tccgctggaa ccatttccgg tgccagagtc actttggtaa
     4981 tcacgtcggc gttctcacac aggaaatcga ccagcgcggc atcaggctta cgcacaaaat
     5041 tcggattatg ggtgcctttt tttaccagat tcagccacgg accttccaga tgcagaccta
     5101 acgcctggtt cggatgtttt gccaggtact cgcgcataac gcgcacgccc tgtttcatca
     5161 gctcatcgct ggtggtgata agcgtcggca gatagttagt acagcctgat ttctcattgg
     5221 ctttctgcat gatttccagc gtttccacgc tgaccgcttc agcggtgtcg ttaaactgta
     5281 cgccgccgca gccgtttaac tgcacatcga taaaaccggg ggagagaatg gccccgttca
     5341 gtgaacgttg ttcgatctct ggcggcagtt ccgctaccgg acagacgctt ttaatcaggc
     5401 catcagcgat aacaaccgcg tggtcatcaa gaaattcgtg gccggtaaag atccggccct
     5461 gggttaatgc atacattctg acccccgatt ttaaaaaata atgccctgag caaggagcca
     5521 gggcagggat aacaattaca gacccttgat attttctgct tctaattcat tgaaatatct
     5581 taaagtctta actttcagct ccatggtgga aggttcatcg cacaccatga tcgcttttgg
     5641 atgcagttgc agacaactga tggtccacat gtggttcacg caaccttcaa cggcggcctg
     5701 cagtgccagt gctttctgac tacccagcac cagaatcatc acttcttcgg catccagcaa
     5761 tgtaccaaca ccgacagtca gggcatattt tggcacctga ttaacatcgt tatcaaagaa
     5821 acgagagttc gcgacgcgag tgtcatgagt cagggttttg atacgagtac gagaagccag
     5881 agaagacgcc ggttcgttaa atgcaatatg accgtcgtta cctacaccgc ccataaacag
     5941 atgaattttt ccgtaagaac ggattttttc ttcatactgg cggcattcgg cgtcgatatc
     6001 cggggcgttg ccgttgagaa ggttgatatt ttctgctgga atatcaacgt gatcgaagaa
     6061 attacggtgc ataaagctgt agtagctttc cggatgctct ttcggcagac cgacatattc
     6121 gtccatgttg aaggtgacaa cgtgcttaaa gctgacctgg cctgctttat gcatttcgac
     6181 taacgcttta taggtggtca tcggcgtgcc gccagtcggc aggcccagta caaacggacg
     6241 atcggcagtc ggtttgaacg cattgatacg attgacgata tggcgagcag cccatttgcc
     6301 gacctgttca gcggtagtca gggggatcag tctcattatt cacctcaata agtaaaatgt
     6361 aagccgttgg cggattaggc atctttaagc gtaacctgga tttgcgcaga caggcgtcaa
     6421 tccgacctga ttttttgaat gataaaataa gttttctggt ttagccagta aaagggagtg
     6481 atgataacga tatttggtga caaaactcac aaaagacacg cgtttaattt gcgatacgaa
     6541 ttaaattttc acacactctg tagcagatga tctaacaatc tgattacaga acatcggcag
     6601 tacaatttgc agcaaaataa aaatacggct ttaaacgagc caaatagggt tctcgtaggg
     6661 ggaataagat gaatatttta ggttttttcc agcgactcgg tagggcgtta cagctcccta
     6721 tcgcggtgct gccggtggcg gcgctgttgc tgcgattcgg tcagccagat ttacttaacg
     6781 ttgcgtttat tgcccaggcg ggcggtgcga tttttgataa cctcgcatta atcttcgcca
     6841 tcggcgtggc atccagctgg tcgaaagaca gcgcaggtgc ggcggcactg gcgggtgcgg
     6901 taggttactt tgtgttaacc aaagcgatgg tgaccatcaa cccagaaatt aacatgggtg
     6961 tactggcggg tatcattacc ggtctggttg gtggcgcagc ctataaccgt tggtccgata
     7021 ttaaactgcc ggacttcctg agcttcttcg gcggcaaacg ctttgtgccg atcgccaccg
     7081 gcttcttctg tctggtgctg gcggccattt ttggttacgt ctggccgccg gtacagcacg
     7141 ctatccatgc aggcggcgag tggatcgttt ctgcgggcgc gctgggttcc ggtatctttg
     7201 gtttcatcaa ccgtctgctg atcccaaccg gtctgcatca ggtgctgaac accatcgcct
     7261 ggttccagat tggtgaattc accaacgcgg cgggaacggt tttccacggt gatatcaacc
     7321 gcttctatgc cggtgacggc accgcgggga tgttcatgtc cggcttcttc ccgattatga
     7381 tgttcggtct gccgggtgcg gcgctggcga tgtacttcgc agcaccgaaa gagcgtcgtc
     7441 cgatggttgg cgggatgctg ctttctgttg ctgttactgc gttcctgacc ggtgtgactg
     7501 agccgctgga attcctgttc atgttccttg caccgctgct gtacctcctg cacgcactgc
     7561 tgaccggtat cagcctgttt gtggcaacgt tgctgggtat ccatgccggc ttctctttct
     7621 ctgcgggggc tatcgactac gcgttgatgt ataatctgcc ggccgccagc cagaacgtct
     7681 ggatgctgct ggtgatgggg gttgtcttct tcgctatcta cttcgtggtg ttcagtttgg
     7741 ttatccgcat gttcaacctg aaaacgccgg gtcgtgaaga taaagaagac gagatcgtta
     7801 ctgaagaggc taacagcaat actgaagaag gtctcaatca actggcaacc aactatattg
     7861 ctgcggttgg cggtactgac aacctgaaag ccattgatgc ctgtattact cgtctgcgcc
     7921 ttaccgtggt tgactcagct cgcgtcaacg atgcgatgtg taaacgtctg ggtgcttctg
     7981 gggtagtgaa actgaacaaa cagactattc aggtgattgt tggcgcgaaa gcagaatcca
     8041 tcggcgatgc gatgaagaaa gtcgttgccc gtggtccggt agccgctgcg tcagctgaag
     8101 caactccggc aactgccgct cctgtagcaa aaccgcaggc tgtaccaaac gcggtatcta
     8161 tcgcggagct ggtatccccg attaccgggg atgttgtggc actggatcag gttcctgacg
     8221 aagcattcgc cagcaaagcg gtgggtgacg gtgtggcggt gaaaccgaca gataaaatcg
     8281 tcgtatcacc agccgcagga acaatcgtga aaatcttcaa caccaaccac gcattctgtc
     8341 tggaaaccga aaaaggcgcg gagatcgtcg tccatatggg tatcgacacc gtagcgctgg
     8401 aaggtaaagg ctttaaacgt ctggtggaag agggcgcgca ggtaagcgca gggcaaccga
     8461 ttctggaaat ggatctggat tacctgaacg ctaacgcccg ttcgatgatt agcccggtgg
     8521 tttgcagcaa tatcgatgat ttcagcggcc ttatcattaa agctcagggc catgttgtgg
     8581 cgggtcaaac accgctgtat gaaatcaaaa agtaatctgc tttatgtctg atgcgacgcg
     8641 gcagcgtcgc atccaacaat aaaaagcggc agggatcttc tctgccgctt tttttgtctg
     8701 agattacatt gggaagataa aaacagcgcc tgcacaaagc gccagaatgc ggatgagata
     8761 cattggtaca gagaacaccc agaattcggg aagtttgtat ttccccattc cgaggatcat
     8821 ggctggcatt ccgtcaatag gcatatagcc gccattccag ccagaaatca ccaccgcaat
     8881 cgcggcagcg gtcgggttaa gcccaaggct agtacaggcg gcaatggcga tgggggcaaa
     8941 aatatatact gaccccatat tcgaacccgt gaaggtggcg caggtactgg taagcaaagc
     9001 aaaggcgaca ataaatacaa acggtgagac gccagaaccg agcacacctg caacggcatc
     9061 gccaaccatc gccgtaaagc cggtgcccgc cagtgcatca gctacaccga tcacccccgc
     9121 catcattaaa atgaccggag cccccatatg atcgcgaact tctttgaagt ccagtacatc
     9181 gatcatcaga acaaacgcac ccgcaagacc cggaataacg aaggcgatat ttcccagaat
     9241 gttcattagc atcatgccga taacgctgac agcgaatgcg gcaatagtgc aatattcttt
     9301 ccaggcagga agggcgttaa acgtttcctc gcgtgccgcc agcgcatcct cggaagcatc
     9361 cgcaatggga tgatccggaa gaaaacggta ggcaataaga ctccatgcga ggaaagccgc
     9421 tgacatgatg agattcacca gcgcaaagcg ccccatcgaa atctcctgat gaaaccccgc
     9481 ggtttcgagt acggtcgccg taacgccaaa gagaagcgcg agattgatag gaagaagcgg
     9541 gtggttggtg gcaaatccca ggggcatcat taatttcgat gttggtagct ttttactgta
     9601 cggaatggtg gataccagcg acagaattag cacgtaataa ccggtcgctc cggtgccggc
     9661 aagactggca cccagcataa caatgactaa caacagccca tagctgcggt aactgccttt
     9721 attgaccagg cttatcatcg cagatttaac gttgctgata agccgggttt tttgtagcgc
     9781 cgccatgacc accataaacc ctgcaatcat caccacgctt ggattgacga atccggcaaa
     9841 agcctgttga acggtcgaaa gtcccgatac aaccagcaat aaacacgcaa gcagtggcgg
     9901 tgcgccaaaa ggcattttgt ccgacatgat cagcacgatc atcaggacca gtattcctaa
     9961 tgccaggatc atttccattg tcattttttc tctcctttta ggcttccagc atgtcgcgcg
    10021 gtactttaaa gaccgctttg cgtattgaag tagccgtgcc aattgcgcac agcgcctggt
    10081 gaggttcctc aggcataaag acggcgaaat caccggcgtg tagggtgatg gcgacactgt
    10141 tttcagggga tggggcgata aacagatcgg gcttacgttc ttcatcgttt tcgcgagcga
    10201 tgggccgcgt tcccgcatta atgccctcac aaccagtaat caccacctgt atatcggccc
    10261 atagatggtg atactccgta tggcggagtt cggcgggttg cgtgtctgaa gttcccagcg
    10321 tgcagaacca ccgacatcct tcaggttgcc agcgaccgtc ctctctggtg gataacgccg
    10381 ataacgaaca ctccgggcgc agcagaatat ttctcaccca ggcaggaagc cccgccagcg
    10441 acaaatggtt gagattaccg ataatcatga tgcctgcccc ttaacccact cgggggagat
    10501 gcgtacgtac ttgatggcct gacgccacca ggtgtaagcg atatgtagcg taccgtccgg
    10561 gctttgtttg atgctggggt aggagaactc acgatttagc ttttccaggg aattgttggt
    10621 catgcagtag ccgtcgcctt catcgagatt gcgccgccac ggccagcttt tcccaccatc
    10681 ggcagaaatc gctaccgtca tcggcgcgcg tggggccccc cagaaagcgg agcgtccgac
    10741 ggctgtcggt tctttgcggc tgtcgtcgcc atcgtcgatt tcgtcataaa gcgacgcgcg
    10801 atgttctact gcgcctgccg cactcatcgc gttgtacacc agcgccagtt cgccgctggc
    10861 aagcgtcgtt acctgaatag acgaattgtt gttgggcaat tcggttgctt gcggcaccga
    10921 ccaactctcg ccgttgtccg cggaatggct gatatagatg ttgtctgccc aacggctgcg
    10981 aaagagagca accaacgtac cgttgtgcag cattgtgata ttcatatgaa cacagccaag
    11041 actttgcggc acctcgacat cgcgccagga gtgaccaccg tcagcggaaa ttttcactgc
    11101 gctgatatca tcattgccga cccatttttc gccgggttgg gtacggcaat aaaagacggg
    11161 cagcagccag ttgccgttat caagcaccgt gattggctgt cgaatgaacg taccgggttt
    11221 atcaagcagg gtggcaatct cgccccaggt ttgtccgagg tcgtctgact tacggtagcg
    11281 gacgatggcg gtgtcctgat taccggatat ttgtgcggtc cataggagcc acagcacgtt
    11341 atccggcgcg agaaacagca ccgggttttg ctctgaacgc gtgtcatcgt gggaaagttt
    11401 gaccgcatcg ctccactgca ttccgccagc gggcaatcgc gatccccaga cagaaatatc
    11461 ggcaatacct tcctgcgttc ccgcaaacca gacgcacatt aacgcgccat ccggcaaagg
    11521 cagaatattc gccgcgtggt tttgcgggca ggacgagggc agcatggcgg taagcacatg
    11581 cgaatcgttt tcggatggac ggatgacgcc gtcacgaata agggctacag acatagttag
    11641 actccatctt aaagttggta atagatttat taatgcgagt gcgattcatg cgtttcggtc
    11701 aatgtcgaaa cttttttatt ttttctttca cggataatat tgagtgtctc aacacttaat
    11761 gagaaaaata tcgcgaagta gagatactct ttggcaatat gaatgttgag actatcggca
    11821 accagcaata caccgacaaa caataaaaag gttaacgcca gtatttttat tgacggcgtg
    11881 gcgttaacga aatcaccaat tgttttcgcc gcaaacatca tgacaccgac ggcaatcatt
    11941 acggcggcaa tcatgataaa tatatgctga gaaaggccga cggcggtgat caccgaatcc
    12001 aggctaaaca cgatatcgag cagcattatc tgtacaatgg caccgcacaa cgacagttgc
    12061 cgcgatccgc tgccatgttc gtcttcctgc gcgggcgaca gctcttcgcg tatctcctgt
    12121 atcgctttat aaatcaggaa cagcccgccc ccgagcagaa tcaaggtgcg gaacgaaatc
    12181 tccagcgtca gcacggtaaa aagcggctgg gtaatatgtg aaagccacgc aatggatatc
    12241 agcaataata tccgcataat catcgcgcag cttaaaccca gtttacgcgc catattttgt
    12301 tgtgataacg gaagttttgc caccaccagt gaaagaaaaa taatattatc caccccaaga
    12361 ataatttcca gaaaggtgag ggtaattaaa gtgagccaga tatctggctg cataaaccac
    12421 aaaaacatgg ggtaatcctt tattctcttt atctaaactt tatagcgggc tggataatag
    12481 agttaattta attaatgtta taatgacgaa taatttgttg aatctcgctg atctgttcag
    12541 gcgtgaaatt aaccgcgtag cggctatcac caacatcatg ccccataagc tgcaacgctt
    12601 ttttcaccgc agccggggag aatgctactt tataaagatc ggtacgcagg cccgttacac
    12661 tctcctgtgc ttcataagac ccttcaatat caccggcatg gaagcgagac cagatggcat
    12721 tgatttctcg cggcgcgatg ttggcgagcc cagagataca ggctgaacat ccctcgacaa
    12781 accctttatg gatcagcgaa tccgggccgt tgagcacatt aaagccgtca atgtcgcgca
    12841 ccgcatcgag aaaacccttc aggctttcgt cgcttccggc gctgtcttta atcccaatga
    12901 tattggggtg cgacgccaga gcacgcgcgg tttctggctg gatcgtattg ccagtgcggg
    12961 ccgggatgtt atagagaaac atcggaatgc tcagtgcgtc ggcaattgcg ctgtaatgcg
    13021 caatcaactc ttcctgtttc agcggtacga agtaaggggc aatgaccgac acggcatcta
    13081 cgccaagttt ttcaatctgc tgacccagac gaatcgtttc gcgcgtagag atctcgccaa
    13141 tgtgtgccac gaccggcgca cgaccagcca cttcatccac gcaggtttgc gtaacgctga
    13201 gcttctcctg ttcattcaac acaaaaaatt cgccgttggt gccgccgcaa aaaatcgcgt
    13261 tacccgcttc cagttgacga ttcacctgca cttttaacgc cgggatattc agactgccgg
    13321 tcgcggtaaa cggggtgacg atagcggtta atacaccttc aatggttttc ggcatgattt
    13381 attccttaaa ttgatgttgc ggaaacagtg tttggtaaat ggcctgtacg tcctgtagat
    13441 caagctggca cggcacattg ttcataaggc gcttcacgtt cagtgccgcc tcgctcagag
    13501 aggcaatatc ctctggcggt acgccgagag ccgcgagatt gttgggtagc ttgagttgat
    13561 tgactaatgc ctgtaaccag gtcaccaggg catgagattt ttcttccgcg ctcagggcgg
    13621 tttccgcatc gggaatgcaa tcccagaccc gggcaaattt ctcgaccgcc caggggcgaa
    13681 caaacgccat gcacggcgcc agcaagatgg cattcgcgac gccatgcggc agatgatatt
    13741 tgccacctaa cgggtaggag agcgcatgaa cgagatgcgt gcccgcatgg gttatcgcta
    13801 cgccgccata gtaagacgcc cacagcattt ccagttttgc gcgcagatcc tgaggatcgt
    13861 tcacggcggg ttgaatgtgc cggaaaagtt tacttaaccc agtcagcgcc gcgttatcgc
    13921 tgaccggatt tgccacggtc gcggtaaaac actccagtaa gtggcaaaga gcatcaatgc
    13981 ccgtggacgc cgcaatatgc gcgggcatgc tggtggtcag ttccgggaaa agcgccacat
    14041 agtctggtaa cagcacctgg gaaataatac ccaccttcgt gctttgctca gggattgcca
    14101 gaatcgcatt cggcgtggct tctgagccgg ttccggcggt tgtaggaatc aaccatgatt
    14161 gcacccgctg agtcggtttt tcacccgcaa gcagcgcatc cagccccggt gattgtggat
    14221 ggcaaagcac cgatagcagc ttcgccacat ccaacacgct gccaccgccg atcccgacca
    14281 ccacatcaaa agaggcatcg ccaggggcat ccattagctg gcgcacatca tgatgcgtgg
    14341 gctctgcggg cacattatcg ataacgttaa cctgcgggca gtgcttttcc agtaaggcgc
    14401 gaatctgctg cacaccgtcg agctgcgcaa tattacggtc agtgaccagc agcatttttc
    14461 tgatgtcggg taagagcgac gtcagggaag ggatagcgcc tgcgccgctc agtatcgtgc
    14521 tattgataat taccacaaat aacctctctt ctgttgattg aattcaatca ttcaaaatgc
    14581 cgtagcctac gtaatatggt ctaattttat aatttattcg agtgattaa
//
LOCUS       DADTFG010000124        14469 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.125, whole genome shotgun sequence.
ACCESSION   DADTFG010000124 DADTFG010000000
VERSION     DADTFG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.125"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            186..431
                     /locus_tag="J1138_003495"
     CDS             186..431
                     /locus_tag="J1138_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455251.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEEVTTINIKSGEQAA"
     gene            428..877
                     /locus_tag="J1138_003496"
     CDS             428..877
                     /locus_tag="J1138_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBA8455252.1"
                     /translation="MTAQISVYGRLVDDPQTKQTSKGTPMTLARMAVSLPYSQSDDGQ
                     ATMWLSVLAFGRQADALAKHHKGELLSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTVRPGGKKGQQGQATDALNRAKQQADQQGSHPPVGDNEQWGDDIPF"
     gene            1095..2135
                     /locus_tag="J1138_003497"
     CDS             1095..2135
                     /locus_tag="J1138_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBA8455253.1"
                     /translation="MVDLYSPTQLVQVVNAVDVQKQLNALFTSLFFTRSVMFESRDII
                     LDTIDDPNIPIAAFCSPMVGSKVSRDEGYESKTIRPGYMKPKSSIDPNKLAVRPAGVS
                     PEQYNAFGARNIKVKQAIVNQAKAIRARIEWLAVQAITTGKNIIEGDGIERYELDWNI
                     KPQNIITQSGGAEWSGKDKETFDPNDDIESYAEFSEGVTNIIIMGGNVWKKYRSFRAI
                     KEALDTRRGSNSELETALKDLGDSVSFKGYMGDVAIVVYSGRYTDEDGTEKHFLDPDL
                     MVLGNTALQGIVAYGGIQDPELIRMGLTKAELAPKNYIVSGDPAIEYVQTHSAPQPIP
                     ARINRFVTVRIG"
     gene            2145..2486
                     /locus_tag="J1138_003498"
     CDS             2145..2486
                     /locus_tag="J1138_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBA8455254.1"
                     /translation="MATHYSELMAGTEALVTTLGIFSANKGVIPAFTPLMQEDATGAL
                     VVWDGSSVGKAVYVSAVQIDTAKKTQAQVYKTGVLNVDALNWPESVKELSVKIAAFVG
                     SGISVQPLARV"
     gene            2498..2881
                     /locus_tag="J1138_003499"
     CDS             2498..2881
                     /locus_tag="J1138_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBA8455255.1"
                     /translation="MQNDYNDLKPIAEMMYPNPAVEELKAIADKMCLSERLVDMNQVM
                     EITTLSRRTLLNLEASGEFPERVQVTEGRKAWYLSEVIDWINNIPRASEYCRVPVPKK
                     PDAALCLKIERVRRNARDGRYKLIG"
     gene            3127..3678
                     /locus_tag="J1138_003500"
     CDS             3127..3678
                     /locus_tag="J1138_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBA8455256.1"
                     /translation="MEANKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLLPGTIEYERHRLTRAQADAQELKNA
                     SETGEVIDTAFATYALSKLTGEVGAIIDSLPLAICRQFPGMEKRYQDFIKMEVSKAFI
                     RAYQISDNIPDMAERYIKDNQRS"
     gene            complement(3785..4690)
                     /locus_tag="J1138_003501"
     CDS             complement(3785..4690)
                     /locus_tag="J1138_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HBA8455257.1"
                     /translation="MRSPNRMHERSTFYKYMSLNTAKIVLDSCSLRWSSPVLFNDPFD
                     VPREVMPGINEINIGKALARKLIAELITPREDIQNLNPRIRTMLSDFQKSFPLGIPAG
                     LIERFQEMVNIPPVGIGAPAAIQEMKDVWRGMLNNRRILCLSESPIITPMWNHYADKY
                     KGIVIEFDCVDFLDSAWLIAKPMKYTDEMPLTHTAEGMAELLFMPDNKSIEYINNEII
                     FIKTEEWAYEREWRISTYARPNSTGQYSDLKFHPFELKSVILGPLFDASEFDEVASLV
                     KMYPRAKLFKSSFGNDRKISIVPLP"
     gene            4851..5615
                     /locus_tag="J1138_003502"
     CDS             4851..5615
                     /locus_tag="J1138_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBA8455258.1"
                     /translation="MTLRRLPDEDPQNLADPAYRRRRIIMQNMRDEELAIAQVEEMQA
                     VSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSGGTEWSKRDKSTYDPTDDIEAYAL
                     NASGVVNIIVFDPKGWALFRSFKAVKDKLDTRRGSHSELETAVKDLGKAVSYKGMYGD
                     VAIVVHSGQYVENGVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINASARYP
                     KNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQLA"
     gene            5657..6055
                     /locus_tag="J1138_003503"
     CDS             5657..6055
                     /locus_tag="J1138_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBA8455259.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDTAGQDTPLSPENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            6067..6420
                     /locus_tag="J1138_003504"
     CDS             6067..6420
                     /locus_tag="J1138_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455260.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            6432..7010
                     /locus_tag="J1138_003505"
     CDS             6432..7010
                     /locus_tag="J1138_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455261.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            7007..7402
                     /locus_tag="J1138_003506"
     CDS             7007..7402
                     /locus_tag="J1138_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455262.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            7410..8150
                     /locus_tag="J1138_003507"
     CDS             7410..8150
                     /locus_tag="J1138_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455263.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVSFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            8166..8588
                     /locus_tag="J1138_003508"
     CDS             8166..8588
                     /locus_tag="J1138_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA8455264.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            8570..9004
                     /locus_tag="J1138_003509"
     CDS             8570..9004
                     /locus_tag="J1138_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA8455265.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            8997..11558
                     /locus_tag="J1138_003510"
     CDS             8997..11558
                     /locus_tag="J1138_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA8455266.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTTQEMLIKAEAAFKK
                     ADDIWSLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDL
                     LRGNWMAGLRSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASDGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGGTGSPAQMRRSEG
                     IRFEQNNNVVIQNDGTNGLPGPQMLKAVYDMARKGARDEIQAQMRDGGLFSGGGR"
     gene            11555..11884
                     /locus_tag="J1138_003511"
     CDS             11555..11884
                     /locus_tag="J1138_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455267.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            11884..12582
                     /locus_tag="J1138_003512"
     CDS             11884..12582
                     /locus_tag="J1138_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA8455268.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGEHYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            12588..13331
                     /locus_tag="J1138_003513"
     CDS             12588..13331
                     /locus_tag="J1138_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455269.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGVIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDLPDFYRHDDWWKSGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASIFV"
     gene            13268..13900
                     /locus_tag="J1138_003514"
     CDS             13268..13900
                     /locus_tag="J1138_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBA8455270.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            13961..>14469
                     /locus_tag="J1138_003515"
     CDS             13961..>14469
                     /locus_tag="J1138_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA8455271.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQTLVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVG"
BASE COUNT         3563 a         3349 c         4294 g         3263 t
ORIGIN      
        1 ttcactgttc acctttttga ttgtttattg agcttcaagg gtgaacagtg gtgaacagtt
       61 ggtgaatagt ttttgtgaaa ctgttcaccc attaacatta tgaattaaaa gagaaaatat
      121 caaaaggtga acaggtgaag ggttaaaacg caaaaatttt aatttactgc tgtgagataa
      181 agcctatgac agcgaagcac acaaaaaaat cacaatcgca cgcccttgat ttgacggaac
      241 actggttaag ggtgtcgata aaaatcatcg accgcaacgc cggggaagga tacgcgaaag
      301 cacatcccga actgattagc gcattcatga caacggcggc tgcaaacttt gccactctga
      361 ccgaacggga gattgctgaa gcggaggaag tgacaacaat caatattaag tccggagagc
      421 aggcagcatg acggcgcaaa tatcagttta cgggcggttg gtggacgacc cgcagacaaa
      481 acagaccagc aagggcaccc ccatgacgct ggcgcgtatg gcggtatcac tgccctacag
      541 tcagtcggat gacggtcagg cgacgatgtg gttatctgtc ctggcgtttg gcagacaagc
      601 cgacgcgctg gcaaagcatc acaaaggcga actcctgagc gtggcgggta atatgcaggt
      661 gagccagtgg actggacaga acggtgaaac gcggcagggc tggcaggtta tcgcagacag
      721 cgtaatcagt gcgcgaacgg tgcgaccggg cggcaaaaaa ggtcaacagg gtcaggctac
      781 tgacgcactg aacagagcaa aacaacaggc agatcagcaa ggaagccatc caccagtggg
      841 agataatgag caatggggag atgatattcc gttttaaata ttgccaataa aaaaggccgg
      901 aaaaaaataa attttccggc atgctacata aatcccgacc aaagggagtg aagatattaa
      961 cactaattat ccgcgctgaa gttgttatcc caaaacttta tacaacattg cactcggttg
     1021 catgtattcg catgacaaat atcggtgata gcatatatcc acaattattt ttaatgaatg
     1081 caaagaggat gcgtatggtt gatttatatt cgcctaccca gcttgtacag gtggttaatg
     1141 ctgtagatgt acaaaaacaa ctaaatgcgt tgtttaccag tttgtttttt actcgctcgg
     1201 taatgtttga atcgcgcgat attattcttg atacaatcga cgatccaaat atcccaattg
     1261 cagcgttttg ttctcctatg gtgggtagta aagtttcacg tgacgaaggg tacgaatcaa
     1321 aaacaattcg tccaggctat atgaagccga aaagcagcat tgatccaaat aagttagctg
     1381 tgcgccctgc tggtgtatca cctgagcaat acaatgcttt tggagcgcgt aatattaaag
     1441 ttaaacaggc gattgtaaat caggctaaag ctattcgtgc acgtattgaa tggcttgccg
     1501 ttcaggcaat cacaacgggg aaaaatatca ttgagggcga tggtattgaa cgttatgagc
     1561 tggactggaa tattaaacca caaaatatca tcactcagtc tggcggtgct gagtggtcag
     1621 gtaaggataa agaaactttt gatccaaatg atgatattga gagctacgca gaatttagtg
     1681 agggcgtcac taatatcatc attatgggcg gtaatgtatg gaagaaatac cgttcattca
     1741 gagcgataaa agaggctctg gatacccgtc gtggttctaa ttccgaactg gaaacggccc
     1801 ttaaagacct tggtgattcg gtgagtttta aagggtatat gggcgatgtt gcgattgttg
     1861 tatacagcgg gcgttatacc gacgaggacg gaactgaaaa acatttcctt gatcctgatt
     1921 tgatggtgct tggcaatacg gctcttcagg ggattgtcgc ctatggcggt attcaggatc
     1981 cggagctaat ccgaatgggg ctgactaaag ccgaacttgc accgaaaaac tatattgtgt
     2041 ctggtgatcc ggctattgaa tatgtgcaga cacattcagc accacagcca ataccggccc
     2101 gcatcaatcg ttttgttacc gttcgcattg gctaaggggg agcaatggct actcattact
     2161 ctgaactcat ggctggcact gaagcactgg tgactacgct ggggatattt tcagctaata
     2221 aaggggtaat tcctgcattt acgccactga tgcaggaaga tgcaacaggt gcactggtgg
     2281 tatgggatgg ttcgagcgta ggtaaagcgg tttatgtttc cgctgtacaa atcgacaccg
     2341 cgaaaaaaac acaggctcag gtctataaga caggtgtctt aaatgttgat gctctgaact
     2401 ggcctgagtc tgtaaaagaa ctgtcagtaa agattgcagc gtttgttggc tcaggtattt
     2461 ctgttcagcc gctggctcgt gtgtaaaggg ggatacaatg cagaatgatt acaatgacct
     2521 taagccaatt gccgaaatga tgtacccgaa tccagctgta gaggaattaa aagctatcgc
     2581 tgacaaaatg tgtttaagcg agcgccttgt tgatatgaat caggtgatgg aaattacaac
     2641 cctgagtcgg cgtacactgc taaaccttga ggctagtgga gagttcccgg agcgtgtgca
     2701 ggttacggaa gggcgtaagg cctggtattt aagtgaagtg atcgactgga taaataatat
     2761 tcctcgcgct tctgaatatt gccgcgtacc tgtcccaaaa aagccagatg cggcgctatg
     2821 cctcaagatt gagcgtgtac gccgcaatgc acgggatggt cgctataagc tgattggttg
     2881 atgaaattag ggcccgctct ggctggcggg tcctttccgg cgatctgaca ggctacgggg
     2941 cgtcaggcgc gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc
     3001 ttcctcgccg taacttaatg tttttattta aaacaccctc tgaaaagaaa ggaaacgaca
     3061 ggtgctaaaa acaggctttt tggcctctgt cgtttccttt ctctggtttt gtccgtggaa
     3121 tgaacaatgg aagccaacaa aaagcagctg gctgacattt tcggtgcgag tatccgtacc
     3181 attcagaact ggcaggagca gggaatgccc gttctgcgag gcggtggcaa gggtaatgag
     3241 gtgctttatg actctgccgc cgtcataaaa tggtatgccg aaagggatgc tgaaattgag
     3301 aacgaaaagc tgcgccggga ggttgaagaa ctgcggcagg ccagcgaggc agatctcctg
     3361 ccagggacta ttgagtatga acgccatcga cttacgcgtg cgcaggccga cgcacaggaa
     3421 ctgaagaatg ccagcgaaac aggtgaggta attgacactg catttgcgac ttatgccttg
     3481 tcgaaattaa ctggggaagt tggagcaatc attgatagtc ttccacttgc gatctgtcga
     3541 cagtttcctg gcatggaaaa acggtatcag gacttcatta agatggaggt cagtaaagca
     3601 tttattcgag cttaccagat ctcagataac attccagata tggctgaaag gtatatcaag
     3661 gataatcagc gaagttaaaa caaatgaaac cataggtagg aagagtgctt aggtggttct
     3721 ttgttgctta aaagtcgcca gtaaaactgg cgatagattt tatttgtcaa aagcatttga
     3781 ccttttaagg tagtggtact atggatattt ttctgtcgtt cccaaatgaa cttttaaata
     3841 atttcgctct tggatacatc ttaactagtg atgcgacttc atcgaactcg ctggcatcaa
     3901 aaagaggacc aagtattaca gattttaact caaaagggtg aaattttaag tcgctatatt
     3961 gaccagtgct atttggccta gcgtaggttg aaattctcca ctctctttca tatgcccatt
     4021 cttctgtttt tatgaatatt atctcattat tgatatattc aattgactta ttgtctggca
     4081 tgaaaagcaa ctcggccatc ccttcagcag tgtgagttag aggcatttca tctgtgtatt
     4141 tcataggttt ggctattaac caagcgctat ccagaaagtc tacacaatca aattcgataa
     4201 ctataccttt atatttatcg gcatagtgat tccacatagg agtgattata gggctctcag
     4261 ataaacacaa aatccttctg ttattaagca ttcctcgcca gacatctttc atttcttgta
     4321 ttgccgcagg agcgccaatc ccaactggtg gaatgtttac catttcttga aatctttcaa
     4381 ttaatcctgc gggtatgcct aaaggaaatg acttttggaa atcacttagc attgttctaa
     4441 ttctagggtt taaattttgt atgtcttctc tgggggtaat caactccgct attaacttgc
     4501 gggctaaagc tttgccaata ttaatttcgt taataccagg cataacttct cttggaacat
     4561 caaaagggtc attaaatagg accggagaac tccatctaag gctgcatgaa tcaagaacaa
     4621 tcttcgcggt attcaacgac atatacttat aaaatgttga gcgttcatgc attctgttgg
     4681 gtgagcgcat agattcctct tgttaaatga gtgatggttt tcatccatgt attgcacgtt
     4741 gtattgcatt caaggatttt ccatttcaat tctatagcta aaacgtaatc aaatcattca
     4801 catcctttca tatttgactc atgtagccga agcatgaggt gaatccgcag atgaccctgc
     4861 gtcgcctgcc ggatgaagat ccgcagaatc tggcggaccc ggcttaccgc cgccgtcgca
     4921 tcatcatgca gaacatgcgt gacgaagagc tggccattgc ccaggtcgaa gagatgcagg
     4981 cagtttctgc cgtgcttaag ggcaaataca ccatgaccgg tgaagccttt gatccggttg
     5041 aggtggatat ggggcgcagt gcggcaaaca acatcacaca gtccggtggc acggagtgga
     5101 gcaagcgcga caagtccacg tatgacccga ccgacgatat cgaagcctac gcgctgaacg
     5161 ccagcggcgt ggtgaatatc atcgtgtttg atccgaaagg atgggcgctg ttccgttcct
     5221 tcaaggccgt caaggataag ctggataccc gtcgcggctc tcattccgag ctggagacag
     5281 cggtaaaaga cctgggcaaa gcggtgtctt ataagggaat gtatggcgat gtggccatcg
     5341 tcgtgcattc cggacagtac gtggaaaacg gcgtcaaaaa gaacttcctg ccggacaaca
     5401 cgatggtgct ggggaacact caggcacgcg gtctgcgtac ctatggctgt attcaggatg
     5461 cggacgcaca gcgcgaaggc attaacgcct ctgcccgtta cccgaaaaac tgggtgacta
     5521 ccggcgatcc ggcgcgtgag ttcaccatga ttcagtcagc accgctgatg ctgctggctg
     5581 accctgatga attcgtgtcc gtacaactgg cgtaatcatg gcccttcggg gccattttct
     5641 ctctgtggag gagtccatga cgaaagatga actgattgcc cgtctccgct cgctgggtga
     5701 gcaactgaac cgtgatgtca gcctgacggg gacgaaagaa gaactggcgc tccgtgtggc
     5761 agagctggaa gaggagcttg atgacacgga tgacactgcc ggtcaggaca cccctctcag
     5821 cccggaaaat gtgctgaccg ggcatgaaaa tgaggtggta tcagcgcagc cggataccgt
     5881 gattcaggat acggctgaac tggtcacggt cgtggcactg gtgacgctgc atactgatgc
     5941 acttcacgcc acgagggata aacctgtggc atttgtgctg ccgggaacgg cgtttcgtgt
     6001 ctctgccggt gtggcagccg aaatgacaga acgtggcctg gccagaatgc aataacggga
     6061 ggcgctgtgg ctgatttcga taacctgttc gatgctgcca ttgcccgcgc tgatgaaacg
     6121 atacgcgggt atatgggaac gtcagccacc atgacatccg gtgagcagtc cggtgctgtg
     6181 atacgtggtg tttttgatga ccctgaaaat atcagctatg ccggacaggg tgtgcgcgtt
     6241 gaaggctcca gcccgtccct gtttgtccgg actgatgatg tgcggcagct gcggcgtgga
     6301 gacacgctga ccatcggtga ggaaaacttc tggatagacc gggtttcgcc ggatgatggc
     6361 ggaagctgtc atctctggct tggacggggc gtaccgcctg ccgttaaccg tcgtcgctga
     6421 aaggaggagg tatggccata aaaggtcttg agcaggccgt tgaaaacctc agccgtatca
     6481 gcaaaacggc ggtgcctggt gccgcagcaa tggccattaa ccgcgttgct tcatccgcga
     6541 tatcgcagtc ggcgtcacag gttgcccgtg agacaaaggt acgccggaaa ctggtaaagg
     6601 aaagggccag gctgaaaagg gccacggtca aaaatccgca ggccagaatc aaggttaacc
     6661 ggggggattt gcccgtaatc aggctgggta acgcgcgggt tgtcctgtcc cgacgcaggc
     6721 gtcgtaaaaa ggggcagcgt tcagccctga aaggtggcgg cagtgtgctt gtggtgggaa
     6781 accgtcgtat tcccggcgcg tttattcagc aactgaaaaa tggccgctgg catgtcatgc
     6841 agcgtgtggc cgggaaaaac cgttacccca ttgatgtggt gaaaatcccg atggcggtgc
     6901 cgcttaccac ggcgtttaaa cagaatattg agcggatacg gcgtgagcgt cttccgaaag
     6961 agctgggcta tgcgctgcag catcaactga ggatggtaat aaagcgatga aacatactga
     7021 actccgagca gccgtactgg atgcactgga gaagcatgac accggggcga cgctttttga
     7081 tggtcgcccc gctgtttttg atgaggaaga ttttccggca attgccgttt atctcaccgg
     7141 cgctgaatac acgggcgaag agctggacag cgatacctgg caggcggagc tgcatattga
     7201 agttttcctg cctgctcagg tgccggattc agagctggat tcgtggatgg agtcccggat
     7261 ttatccggtg atgagcgata tcccggcact gtcagatttg atcaccagta tggtggccag
     7321 tggctatgac taccggcgcg acgatgatgc gggcctgtgg agttcagccg atctgactta
     7381 tgtcattacc tatgaaatgt gaggacgata tgcctgtacc aaatccagta atgccggtga
     7441 aaggggccgg gaccacactg tgggtttata aggggaacgg tgacccttat gcgaacccgc
     7501 tttcagacgt tgactggtcg cgtctggcta aagttaaaga cctgacgccc ggcgaactga
     7561 ccgctgagtc ctatgacgac agctatctcg atgatgaaga tgcggactgg actgcgaccg
     7621 ggcaggggca gaaatctgcc ggagatacca gcttcacgct ggcgtggatg cccggagagc
     7681 aggggcagca ggcgctgctg gcgtggttta atgaagggga tacccgtgcc tataaaatcc
     7741 gcttcccgaa cggcacggtc gatgtgttcc gcggctgggt cagcagtatc ggtaaggcgg
     7801 tgacggcgaa ggaagtgatc acccgcacgg tgaaagtcac caacgtggga cgtccgtcga
     7861 tggcagaaga tcgcagcacg gtaacagcgg caaccggcat gaccgtgacg cctgccagca
     7921 cttcggtggt gaaagggcag agcaccacgc tgaccgtgtc attccagccg gaaggcgcaa
     7981 ccgacaagag cttccgtgcg gtgtctgcgg ataaaacaaa agccaccgtg tcggtcagtg
     8041 gtatgaccat caccgtgaaa ggtgttgctg caggcaaggt caacattccg gttgtatccg
     8101 gtaatggtga acttgctgtg gttgcagaaa tcaccgtcac cgacagttaa tccggagagt
     8161 cagcgatgtt cctgaaaacc gaatcatttg aatataacgg tgtgagcgtc acgctttctg
     8221 aactgtcagc cctgcagcgt attgagcatc tcgccctgct gaaacgacag gcagaacagg
     8281 cgggatccag tctcaatcga caggtgagcg tggaagatct cgtcagaacc ggtgcttttc
     8341 tggtggcgat gtccctgtgg catagccatc cgcagaagac aaagatgccg tccatgaatg
     8401 aagccgttaa acaaattgag caggaagtgc ttaccacctg gcccacagag gcaattgctc
     8461 aggctgaaaa tgtggtaatg cgtctgtccg gtatgtctga gtttgttgtg aatgatgcac
     8521 ctgaacaggc agatgacgcc gggcccgcag agcctgtttc tgcgggaaag tgttcgacgg
     8581 tgagctgagt tttgccctga aactggcgcg tgagatgggg cgacccgact ggcgcgccat
     8641 gcttgccggg atgtcatcca cggagtatgc cgactggcac cgcttttaca gtacccatta
     8701 ttttcatgat gttctgctgg atatgcactt ttccgggctg acgtacaccg tgctcagcct
     8761 gtttttcagc gatccggata tgcatccgct ggatttcagt ctgctgaacc ggcgcgaggc
     8821 tgacgaagag cctgaagatg atgtgctgat gcagaaagcg gcagggcttg ccggaggcgt
     8881 tcgttttggc ccggacggga atgaagttat ccccgcttcc ccggatgtgg cggacatgac
     8941 ggaggatgac gtaatgctga tgacagtatc agaagggatc gcaggaggag tccggtatgg
     9001 ctgaaccggt aggcgatctg gtcgttgatt taagtctgga tgcggccaga tttgacgagc
     9061 agatggccag agtcaggcgt catttttccg gtacggaaag tgatgcgaaa aaaacagcgg
     9121 cagtcgttga acagtcgctg agccgacagg cactggctgc acagaaagcg gggatttccg
     9181 tcgggcagta taaagccgcc atgcgtatgc tgcctgcgca gttcaccgac gtggccacgc
     9241 agcttgcagg cgggcaaagt ccgtggctga tcctgctgca acagggtggt caggtgaagg
     9301 actccttcgg cgggatgatc cccatgttca gagggcttgc cggtgcgatc accctgccga
     9361 tggtcggggc cacctcgctg gcggtggcga ccggtgcgct ggcgtatgcc tggtatcagg
     9421 gcaactcaac cctgtccgat ttcaacaaaa cgctggtcct ttccggcaat cagtcgggtc
     9481 tgacggcaga tcgtatgctg gtcctgtcca gagccgggca ggcggcaggg ctgacgttta
     9541 accagaccag cgagtcactg acggcgctgg tgaatgcagg tgtgcgtggt ggtgagcagt
     9601 ttgaggcgat cagccagagt gtggcgcgtt tctcctctgc atccggcgtg gaggtggaca
     9661 aggtcgctga agccttcggg aagctgacca cagacccgac gtcgggactg acagcgatgg
     9721 cacgtcagtt ccataacgtg acggcggagc agattgcgta tgttgctcag ttgcagcgtt
     9781 ccggcgatga agccggggca ttgcaggcgg cgaacgaggc cgcgacgaaa gggtttgatg
     9841 accagacccg ccgcctgaaa gagaacatgg gtacgctgga gacctgggca gacaggacag
     9901 cgcgggcatt caaatccatg tgggatgcgg tgctggatat tggtcgtcct gataccacgc
     9961 aggagatgct gattaaggca gaggctgcgt ttaagaaagc ggacgacatc tggagtctgc
    10021 gcaaggatga ttattttgtt aacgatgaag cgcgggcgcg ttactgggat gatcgtgaaa
    10081 aggcccgtct tgcgcttgaa gccgcgagaa agaaggctga gcagcagact caacaggaca
    10141 aaaatgcgca gcagcagagc gataccgaag cgtcacggct gaaatatacc gaagaggcgc
    10201 agaaggctta cgaacgcctg cagacgccgc tggagaaata taccgcccgt caggaagaac
    10261 tgaataaggc actgaaagac gggaaaatcc tgcaggcaga ttacaacacg ctgatggcgg
    10321 cggcgaaaaa ggactatgaa gcgacgctga aaaagccgaa acagtccggc gtgaaggtgt
    10381 ctgcgggcga tcgtcaggaa gacagtgctc atgctgccct gctgacgctt caggcagaac
    10441 tccggacgct ggagaagcat gccggagcga atgagaaaat cagccagcag cgccgggatt
    10501 tgtggaaggc ggagagtcag ttcgcggtac tggaggaggc ggcacaacgt cgccagctgt
    10561 ccgcacagga gaaatccctg ctggcgcata aagatgagac gctggagtac aaacgccagc
    10621 tggctgcact tggcgataag gtcacgtatc aggagcgcct gaatgcgctg gcgcagcagg
    10681 cggataaatt cgcacagcag caacgggcaa aacgggccgc cattgatgcg aaaagccggg
    10741 ggctgactga ccggcaggca gcgcgggaag ccacagaaca gcgcctgaag gaacagtatg
    10801 gcgataatcc tctggcgctg aataacgtca tgtcagagca gaaaaagacc tgggcggctg
    10861 aagacctgct tcgcgggaac tggatggcag gcctcaggtc cggctggagc gagtggaaag
    10921 agagtgccac ggacagtatg tcgcaggtta aaagtgctgc cacgcagacc tttgatggta
    10981 ttgcgcagaa tatggcggcg atgctgaccg gcagtgaaca gaactggcgc agcttcaccc
    11041 gctcagtgct gtccatgatg acggaaattc tgcttaagca ggcaatggtg gggattgtcg
    11101 ggagtatcgg cagcgccatt ggcggggctg ttggtggcgg cgcatcagcg tcagacggta
    11161 cagccattca ggctgctgcg gcgaaattcc attttgcgac cggaggattt acgggaaccg
    11221 gcggcaaata tgagccagcg gggattgttc accgtggtga atttgtcttc acgaaggagg
    11281 caaccagccg gattggcgta ggaaatcttt accggctgat gcgcggctat gccaccggtg
    11341 gttatgtcgg tggcaccgga agtccggcgc aaatgcggcg ttcagagggt atcagatttg
    11401 agcagaacaa caacgtggtg attcagaacg acggtacgaa tggtctgcca ggtccacaga
    11461 tgctgaaggc agtgtatgac atggcccgca agggtgcccg tgatgaaatt caggcacaga
    11521 tgcgcgatgg tggtctgttc tccggaggtg ggcgatgaag accttccgct ggaaagtgaa
    11581 acccggtatg gatgtggctt cggccccttc cgtcaggaaa gtgcgctttg gtgatggcta
    11641 ttcccagcga gcgcctgccg ggctgaatgc cgacctgaaa acgtacagcg tgacgctttc
    11701 tgttccccgt tgggaggcca cggcgctgga gtcgtttctg gctgagcacg ggggctggaa
    11761 agccttcctg tggacgccgc cttatgagtg gcggcagata aaggtgacct gcgcaaaatg
    11821 gtcgtcgcgg gtcagtatgc tgcgtgttga gttcagcgca gagtttgaac aggtggtgaa
    11881 ctgatgcagg atatccggca ggaaacactg aatgaatgca cccgtgcgga gcagtcggcc
    11941 agcgtggtgc tctgggaaat cgacctgaca gaggtcggtg gagaacatta ttttttctgt
    12001 aatgagcaga acgaaaaagg tgagccggtc acctggcagg ggcgacagta tcagccgtat
    12061 cccattcagg ggagtggttt tgaactgaat ggcaaaggca ccagtacgcg ccccacgctg
    12121 acggtttcta acctgtacgg tatggttacc ggtatggtgg aagatatgca gagtctggtc
    12181 ggcggaacgg tggtcaggcg taaggtttac gcccgttttc tggatgcggt gaacttcgtc
    12241 aacggaaaca gcgacgccga tccggagcag gaggtgatca gtcgctggcg catcgagcag
    12301 tgcagcgaac tgagcgcggt cagtgcctcc tttgtactgt ccacgccgac ggaaacggac
    12361 ggcgctgttt ttccgggacg tatcatgctg gccaacacct gcacctggac ctatcgcggc
    12421 gatgagtgcg gttatagcgg tccggcggtc gcggatgaat atgatcagcc gacgtccgat
    12481 atcacgaagg ataaatgcag caaatgcctg agtggctgta agttccgcaa taacgtcggc
    12541 aactttggcg gcttcctttc cattaacaaa ctttcgcagt aaatcccatg acagagacag
    12601 aatcagcgat tctggcgcac gcccggcgat gcgcgccagc ggagtcgtgc ggcttcgtgg
    12661 tgagaacgcc ggagggggaa agatattttc cctgcgtgaa tatctccggt gagccggagg
    12721 cgtatttccg tatgtcgccg gaagactggc tgcaggcaga aatgcagggt gagattgtgg
    12781 cgctggtcca cagccacccc ggtggtctgc cctggctgag tgaggccgac aggcggctgc
    12841 aggtgcagag tgatttgccg tggtggctgg tctgccgggg agtgattcat aagttccgct
    12901 gtgtgccgca tctcaccggg cggcgttttg agcacggggt gacagactgt tatacgctgt
    12961 tccgcgatgc ctatcatctt gcaggtatcg atttgccgga tttttaccga catgatgact
    13021 ggtggaaatc aggtcagaat ctctatctgg ataatctgga ggccacaggg ctgtatcagg
    13081 tggcgttgtc atcagcacaa ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc
    13141 cgaatcatgc cgccatttac tgtggtgatg gcgagctgct gcaccatatt cctgaacaac
    13201 tgagcaaacg agagaggtat accgacaaat ggcagcgacg cacacactcc ctctggcgtc
    13261 accgggcatg gcacgcatct gcctttacgg ggatttacaa cgatttggcc gccgcatcga
    13321 tcttcgtgtg aaaacggggg ctgaagctat ccgggcgctg gccacacagc tcccggcgtt
    13381 tcgtcagaaa ctgagcgacg gctggtatca ggtacggatt gccggacggg atgtcagcac
    13441 gtccggatta acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt
    13501 tcccagagtc gccggggcca agtcaggtgg cgtattccag attgtcctgg gagctgccgc
    13561 cattgccgga tcattcttta ctgccggagc cacccttgca gcatgggggg cagccattgg
    13621 ggccggtggt atgaccggta tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt
    13681 ggcgcagatg ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggtaa
    13741 gcagaacacg tatttctctt cactggataa catggttgcc cagggtaatg ttctgcccgt
    13801 tctgtacggt gaaatgcgcg tggggtcacg cgtggtttct caggagatca gcacggcaga
    13861 cgaaggggac ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa
    13921 accgcctgcg ggcggttttg tcgtttatgg agcgtgagga atgggtaaag gcagcagtaa
    13981 ggggcatacc ccgcgcgaag cgaaggacaa cctgaagtcc acgcagttgc tgagtgtgat
    14041 cgatgccatc agcgaagggc cgattgaagg tccggtggat ggattaaaaa gcgtgctgct
    14101 gaacagtacg ccggtgctgg acactgaggg gaataccaac atatccggtg tcacggtggt
    14161 gttccgtgcc ggtgagcagg agcagacacc gccggagggg tttgaatcct ccggctccga
    14221 gacggtgctc ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc
    14281 aaacattgac cgtctgcgtt ttactttcgg cgtgcagaca ctggtggaaa ccacctcaaa
    14341 gggggacagg aatccgtcgg aagtccgcct gctggttcag atacagcgta acggtggctg
    14401 ggtgacggaa aaagacatca ccattaaggg caaaaccacc tcgcagtatc tggcctcggt
    14461 ggtggtggg
//
LOCUS       DADTFG010000125        14411 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.126, whole genome shotgun sequence.
ACCESSION   DADTFG010000125 DADTFG010000000
VERSION     DADTFG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.126"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(248..1897)
                     /gene="pgi"
                     /locus_tag="J1138_003516"
     CDS             complement(248..1897)
                     /gene="pgi"
                     /locus_tag="J1138_003516"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBA8455272.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2422..3771
                     /gene="lysC"
                     /locus_tag="J1138_003517"
     CDS             2422..3771
                     /gene="lysC"
                     /locus_tag="J1138_003517"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBA8455273.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4024..4296
                     /locus_tag="J1138_003518"
     CDS             4024..4296
                     /locus_tag="J1138_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBA8455274.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4297..5169)
                     /gene="rluF"
                     /locus_tag="J1138_003519"
     CDS             complement(4297..5169)
                     /gene="rluF"
                     /locus_tag="J1138_003519"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBA8455275.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5517..6464
                     /locus_tag="J1138_003520"
     CDS             5517..6464
                     /locus_tag="J1138_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBA8455276.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDIQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6534..7340
                     /locus_tag="J1138_003521"
     CDS             6534..7340
                     /locus_tag="J1138_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA8455277.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEINHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7350..7757
                     /locus_tag="J1138_003522"
     CDS             7350..7757
                     /locus_tag="J1138_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose IIA component"
                     /protein_id="HBA8455278.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7757..8251
                     /locus_tag="J1138_003523"
     CDS             7757..8251
                     /locus_tag="J1138_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBA8455279.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8317..9114
                     /locus_tag="J1138_003524"
     CDS             8317..9114
                     /locus_tag="J1138_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBA8455280.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            9125..9949
                     /locus_tag="J1138_003525"
     CDS             9125..9949
                     /locus_tag="J1138_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA8455281.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10001..11230
                     /locus_tag="J1138_003526"
     CDS             10001..11230
                     /locus_tag="J1138_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBA8455282.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLPTD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11612..12301
                     /gene="pepE"
                     /locus_tag="J1138_003527"
     CDS             11612..12301
                     /gene="pepE"
                     /locus_tag="J1138_003527"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBA8455283.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12391..14022)
                     /locus_tag="J1138_003528"
     CDS             complement(12391..14022)
                     /locus_tag="J1138_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBA8455284.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGAVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         3523 a         3627 c         3750 g         3511 t
ORIGIN      
        1 aggattaacc tcacggtatg attttcgtta aatgacagac aagaggattt atctgataaa
       61 acaatgccga cttatagtcg gcattgtttt taaggctatt tgatcaatcg tactatgtat
      121 taattgccga atacgatgta catatcggca tcgacctgta ggcctgataa gacgcgaccg
      181 cgtcgcatca ggcatcggtt gccggatgcg gcgtgaacgc cttatccggc ctacatatcg
      241 acaatgatta accgcgccac gctttatagc ggttaatcag accattggtc gagctatcgt
      301 ggctgctgat ttctttatca tctttcagct ctggcagaat acggttcgcc agttgtttac
      361 ccagctccac gccccactgg tcgaaggtga agatgttcag gatcacgccc tgagtaaaga
      421 ttttgtgctc atacagcgca atcaacgccc ccaggctgaa cggagtgatt tcacgcagca
      481 ggatggagtt agtcgggcgg ttaccttcga acactttgaa cggcaccacg tagtcaagcg
      541 ttgccggatc tttaccctga tcgcgatatt cctgctcaac cacttcgcgg gatttaccaa
      601 acgccagcgc ttcggtctga gcaaagaagt tagacagcag tttctggtga tgatcagaga
      661 gcgggttatg ggtgatagcc ggagcgatga aatcgcacgg taccattttg gttccctggt
      721 ggatcagctg atagaacgcg tgctgaccgt tcgtgccagg ttcaccccag ataatcgggc
      781 cagtctggta atccacaacc ttaccgttac ggtcaacata cttaccgtta gactccatat
      841 tgccctgctg gaagtacgcc gcgaaacggt gcatatactg gtcatacggc agaatcgctt
      901 cagtttccgc gccaaagaaa ttgttgtacc agatgccaat cagcgccagc agtacaggca
      961 ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat cgcgtgtgcg ccagaaagca
     1021 gttcaacgaa gttatcaaag ccgatggaga gaacaatcga caggccaatc gctgaccaca
     1081 aagagtaacg accgccgacc cagtcccaga attcgaacat gttggcagta tcaataccaa
     1141 actcgccaac ggctttggcg ttggtggaaa gcgccgcaaa gtgtttcgca acgtgtttct
     1201 catcacctgc cgctttcagg aaccagtcac gcgcgctatg ggcgttggtc atggtttcct
     1261 gagtggtgaa ggttttagat gctaccagga acagcgtggt ttccgggttt acttttttca
     1321 gcacttccgc gatgtgagtc ccatcgacgt tagaaacaaa gtgcatgttc aggtggtttt
     1381 tgtatggacg cagtgcttca gtcaccatgt acgggccgag gtcagaaccg ccgatcccga
     1441 tgttcactac gtcagtgatt gccttgccgg tatagccttt ccactcaccg gaaataatcg
     1501 cttccgagaa ggttttcatc ttctccagca ctgcgttgac ttccggcata acatctttgc
     1561 catcaaccag aatcggggta ttgctacggt tacgcagcgc tacgtgcagt acggcgcggt
     1621 tttcagtgcg gttgatcttc tcgccagaga acatcgactt aatcgcgccc gccagatcgc
     1681 actctttcgc cagatcttgt aatttcgcca gcgtctcttc agtgatgcgg tttttggagt
     1741 aatccaccag catctgatcg tcgaaggttg cggagaactt agaaaaacga tcgccgtctt
     1801 tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc gaagtgtttc tgtagtgcct
     1861 gccaggcagc ggtctgcgtt ggattgatgt ttttcattag caatactctt ctgattttga
     1921 gaattgtgac tttggaagat tgtagcgcca gtcacagaaa aatgtgatgg ttttagtgcc
     1981 gttagcgtaa tgttgagtgt aaacccttag cgcagcgaag catttattag ctgaactact
     2041 gaccgccagg agtggatgaa aaatccgcat gaccccatcg ttgacaaccg ccccgctcac
     2101 cctttattta taaatgtact acctgcgcta gcgcaggcca gaagaggcgc gttgcccaag
     2161 taacggtgtt ggaggagcca gtcctgtgat aacacctgag ggggtgcatc gccgaggtga
     2221 ttgaacggct ggccacgttc atcatcggct acaggggctg aatcccctgg gttgtcacca
     2281 gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac ttctgggtga aaatagtagc
     2341 gaagtatcgc tctgcgccca cccgtcttcc gctcttccct tgtgccaagg ctgaaaatgg
     2401 atcccctgac acgaggtagt tatgtctgaa attgttgtct ccaaatttgg cggtaccagc
     2461 gtagctgatt ttgacgccat gaaccgcagc gctgatattg tgctttctga tgccaacgta
     2521 cgtttagttg tcctctcggc ttctgctggt atcactaatc tactggtcgc tttagctgaa
     2581 ggactggaac ctggcgagcg attcgaaaaa ctcgacgcaa tccgcaatat ccagtttgcc
     2641 attctggaac gtctgcgtta cccgaacgtt atccgtgaag agattgaacg tctgctggag
     2701 aatattactg ttctggcaga agcggcggcg ctggcaacgt ctccggccct gacagatgaa
     2761 ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg tcgagatcct gcgcgaacgc
     2821 gatgttcagg cacagtggtt tgatgtacgt aaagtgatgc gtaccaacga tcgatttggt
     2881 cgtgcagagc cagatgtagc cgcgctggcg gaactggccg cgctgcagct gctcccacgc
     2941 ctcaatgaag gcttagtgat cacccaggga tttatcggca gcgaaaataa aggtcgtaca
     3001 acgacgcttg gccgtggagg cagcgattat acggcagcct tgctggcgga ggctttacac
     3061 gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct acaccaccga tccgcgcgtg
     3121 gtttccgcag caaaacgcat tgatgaaatc gcgtttgccg aagcggcaga gatggcaact
     3181 tttggtgcaa aagtactgca tccggcaacg ttgctacccg cagtacgcag cgatatccca
     3241 gtctttgtcg gctccagcaa agacccacgc gcaggtggta cgctggtgtg caataaaact
     3301 gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca atcagactct gctcactttg
     3361 cacagcctga atatgctgca ttctcgcggt ttcctcgcgg aagttttcgg catcctcgcg
     3421 cggcataata tttcggtaga cttaatcacc acgtcagaag tgagtgtggc attaaccctt
     3481 gataccaccg gttcaacctc cactggcgat acgttgctga cgcaatctct gctgatggag
     3541 ctgtctgcac tgtgccgggt ggaagtggaa gagggtctgg cgctggtcgc gttgattggc
     3601 aatgacctgt caaaagcctg cggcgttggc aaagaggtat tcggcgtact ggaaccgttc
     3661 aacattcgca tgatttgtta cggcgcatcc agccataacc tgtgcttcct ggtgcccggc
     3721 gaagatgccg agcaggtagt gcagaagctg cattttaatt tgtttgagta aacacagtat
     3781 ggcccggaag acattatttc gggccgtatt gtttttcttg tcattatgcc cataaataaa
     3841 cgagtctatt ccctgttaac cagcgtcttt atcggagaat aattgccttt aattttttat
     3901 ccgcatctct aactaattat cgaaagagat aaatagttaa gagaaggcaa aatgaatatt
     3961 atcagttctg ctcgcaaacg aattcccgta cactgaactc tctggctcac tgagggcacc
     4021 atcatggcac tcccccgtat tacccaaaaa gagatgactg aacgcgagca gcgtgaactt
     4081 aaaacactgc tggatcgggc gcggattgcg catggtcgcg tactgaccaa ctccgaaact
     4141 aacagcatta agaaagagta catcgataaa ttaatggtcg agcgtgaggc tgaagcgaaa
     4201 aaagcccgcc aattgaagaa aaagcaggct tataaaccgg atcctgaggc gtcatttagc
     4261 tggtcagcaa atacgtcgac gcgcggcagg cgctgattaa cgccccttct ttttacgccc
     4321 cggcgaggta aaacgcttac cgttggacgc cgggcgaccg cctttttccg ccgttttttc
     4381 catcttaacg actggacgtt tgatgcctac cgttttcggt ttggccttcg ctttaggttt
     4441 tgcctcggaa gaggaatttt caatgagctt aaagagatcg attaactcat cgtcggttaa
     4501 atcgcgccac tcacccagcg gaatgccgct taagctgacg ttcataatgc gcgtacgttc
     4561 cagctttttc acttcgtaac caaagtgctc gcacatgcga cgaatctgac ggttcagccc
     4621 ctgcaccagg gtaatgcgga agacaaacgg tgcttctttt ttgactttgc actttttagt
     4681 tactgtcccg aggatcggta ctcccgcccc cataccacga ataaattcat cggtaatcgg
     4741 tttatcaacc gtcaccagat actctttctc atgatcgtta cccgcacgga ggatcttatt
     4801 caccagatcg ccgtgattgg tgaggaaaat cagcccctgg gaatctttat ccaggcggcc
     4861 aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc gctcgccatc
     4921 ttcggtggtg ctgacaatac cgacgggctt gttcagggcg ataagtacca aatcttcgga
     4981 ttcccgaggc tcaatcaact gaccatttac tttcacaacg tcgccgggtt tcacctgatc
     5041 gccaatggtg gctcgcttgc cattaaggaa cacattgcct tgctcgatat agcgatccgc
     5101 ttcgcggcgt gagcaaattc cgctttcgct gatgtattta tttaaacgga ctgatgagtc
     5161 gggcagcata aattctcctg taaaacgcgg aatataccgt acctcttagc ggggaaaaac
     5221 agggttatgc ggcaacgtcg aaattaaatg tgatctgcca cgccttttat cacaaaagtg
     5281 tctccggaag tacgatgcaa atttgcctgg caaaataaga ataatcaaca aattacgcat
     5341 cgctgcacac ctgtgctgat gactactacg cagagaaaca aatgtgcaag agaaagcgct
     5401 atttcccccc atgcaaagag tgaaaaatcg ctttgaaagg ccatcatgat ggggttatat
     5461 cagatcaagc ggtgagaaag cgtatttgca caaatgtgca gaaccaggag gcggcaatgg
     5521 aaaacagtga cgatatccgt ttgattgtga agattgccca actctattac gaacaggata
     5581 tgacgcaggc gcaaattgcg cgcgaactgg gtatttatcg cactaccatc agccgcttgc
     5641 ttaaacgagg ccgcgatcag ggaattgtca ccatcgccat caactatgac tacaacgaaa
     5701 atctctggct ggagcagcaa gtgaagcaaa agtttggcct gaaagacgtt gtggtggtgt
     5761 caggaaatga tgaggatgaa gatattcaac tggcgatgat ggggttacac ggcgcgcaac
     5821 tgctggatcg cttgctggaa cctggcgata ttgtcggttt ttcctggggc cgcgcggtga
     5881 gcgcactggt tgaaaacttg ccgcaggcgg ggcaatcgcg gcagttaatt tgcgtgccga
     5941 ttattggcgg cccgtccggt aaactcgaaa gccgctatca cgtaaacaca ttaacctaca
     6001 gcgcggcagc gaagctgaaa ggggaatcgc atctcgcgga ttttccggcg ctgctggata
     6061 acccattaat tcgtaatggg atcatgcagt ctcagcactt taaaaccatc tctgcctact
     6121 gggataatct ggatgtcgcc ctggtgggaa ttggctcacc ggccattcgc gacggcgcta
     6181 actggcatgc gttttatggt ggtgaagaga gtgacgacct gaatgcccgc caggttgctg
     6241 gcgatatttg ctcgcgcttt tttgatattc acggcgcaat ggttgaaacg aatatgagcg
     6301 aaaaaacact ctctatcgaa atgaataaat taaagcaggc acggtattcc attggcattg
     6361 ccatgagcga agaaaaatac agcggaattg ttggtgcact gcgtggaaaa tatattaatt
     6421 gtctggtaac gaatagcagc acagctgaac tattactgaa ataaaaatct tcccgatgtt
     6481 taccgtaata aaaattacgg cgggtcccta ctatctaaaa tcaggagtct gttatgcaaa
     6541 cgtggttaaa tttgcaggat aaaatcatta ttgtcaccgg tggcgcatcc ggtattggtc
     6601 tggcgattgt agaggaatta ttagcacaag gcgcgaatgt acagatggtc gatattcacg
     6661 gtggcgatgg tcaatatgaa agccataaag gttatcagtt ctggccgacc gatatttcca
     6721 gcgccaaaga gataaatcat acggtagcag aaattatcca gcgttttggt cgcatcgacg
     6781 gtctggttaa caacgccggg gtcaatttcc cgcgtctgct ggtcgatgag aaagcgcctg
     6841 ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat ggtcaatatc aaccagaaag
     6901 gcgtttttct gatgtcgcag gcggtggcgc gacagatggt caaacaacat gatggcgtga
     6961 ttgtgaatgt ttcctcagaa agtgggctgg aaggctcaga aggccaaagc tgttacgccg
     7021 cgaccaaagc cgcgctcaat agcttcacgc gctcctggtc gaaagagctg ggcaagcacg
     7081 gtatccgtgt ggtcggtatc gcgccgggga ttctggaaaa aacaggactg cgcacgccgg
     7141 aatatgaaga agcgctggcg tggacgcgca atatcaccgt cgagcagctg cgtgaaggct
     7201 ataccaaaaa cgccattcct attgggcgcg ccggaagatt agcagaagtg gctgattttg
     7261 tttgttatct gctgtctgaa cgcgccagct atatcaccgg agtaaccact aacattgcag
     7321 gcggcaaaac gcgcggctaa ggaggcttta tggtcaatgc cattttttgc gcccacggca
     7381 aattagcctg cgccatgctg gaatcggttc agatggtgta cggcgatgcc agggtcgagg
     7441 ccgttgaatt tgtgcccggc gagaacgccg gagacattgt ggcaaagctg gagaagttag
     7501 taagcattca caatcaagat gagtggctaa tcgccgtgga tttacagtgc ggcagcccgt
     7561 ggaatgcggc agcgatgctt gcgatgcgca atcctcgtct gcgggtaatc agcggccttt
     7621 ctctgccgct ggcgctcgaa ctggttgata accaggacag catgaatatt gatgaactgt
     7681 gtgaacacct gacgcaaatc gcaaagcaaa cctgcgtcgt ctggagacag gtggcaacag
     7741 cggaggagga tttctgatga acattacgct cgcccgcatt gatgaccgcc tgatccacgg
     7801 ccaggtcact accgtctggt ctaaagtggc taacgcccaa cgcattatta tctgcaatga
     7861 cgaagtttat aacgatgaag ttcgccggac attattgcgt caggctgcgc caccgggaat
     7921 gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt tatcacaacc cgcaatatca
     7981 ggacgaaacc gttttttatc ttttcactcg cccacaggat gccttagcaa tggttcgcca
     8041 gggggtaaaa ataggtaccc tgaatattgg cggtatggcc tggcgtcctg gtaagaaaca
     8101 gttaaccaaa gcagtttcat tagatgacga tgatattaac gcatttcatg agttaaataa
     8161 tcttggcgtc atactggatt tacgcgtcgt agcgtcagat ccctcaatca atattatcga
     8221 caaaataaac gaacagttaa tcgcaaatta aataatagcg cctgtgtaca tatgctttaa
     8281 caggcaggga tgacgacact taaaaggtga caaatcatgg aaataagtac cctacaaatc
     8341 attgctatat ttcttttttc ctgtattgcc ggaatgggca gcgtgctgga tgaatttcag
     8401 actcatcgtc cgttaattgc ctgtacggtg attggtttaa ttctcggtga tttaaaaacc
     8461 ggaattatgc tcggtggtac gctggaattg atagctctcg gctggatgaa cgtcggcgcg
     8521 gcgcaatctc cggattctgc actcgccagc ataatctccg ccattctggt tatcgttggt
     8581 cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg tggctgcggc aggccaggtg
     8641 ctgacggtgt ttgcccgtac catcaccgta gtgttccagc acgcggcgga taaagcagcg
     8701 gaagaagcac ggtttcgcac tctcgatatt ctgcatgtct ccgcgcttgg cgtgcaggca
     8761 ctgcgcgttg ctattccggc actgattgtc tcgctgttcg tcagcgctga tatggtgagc
     8821 aatatgctga gcgccattcc cgaatttgtg acccgtggac tgcagattgc tggcggtttt
     8881 atcgtggtgg tcggttacgc catggtgctt cgcatgatgg gcgtgaaata tttgatgcct
     8941 ttctttttcc tcggtttcct cgcaggtggc tacctcgatc tcagtctgct ggcgttcggt
     9001 ggcgtcggcg tgatcatggc cctgctctac atccagttaa atccacagtg gcgtaaagct
     9061 gaaccacacc cccagaccac cactatcacc gcccttgacc aacttgatga ttaacggagc
     9121 catcatggaa cagagaaaaa ttacacgcag cgatctggtg agcatgtttc tgcgctccaa
     9181 cctgcaacag gcatccttta actttgaacg tattcacggg ctgggttttt gctacgacat
     9241 gatccccgcc atcaaacgac tttacccatt aaaagaggat caggttgcgg cgctcaggcg
     9301 acacctggtg ttcttcaata ccacgccagc cgtatgtggc ccggtcatcg gcgtcactgc
     9361 cgccatggaa gaggcgcgag ccaacggcgc ggaaattgat gacggtacca ttaacggcat
     9421 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     9481 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     9541 cctgctgttc ttctttattt tcaacgcggt gcgtctggcg atgaagtggt atggcctaca
     9601 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcggtaatt tgctgcaaaa
     9661 actcaccgaa ggcgcgtcga ttctcgggct gtttgtgatg ggcgtgctgg tgaccaaatg
     9721 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcct gccgccgatg gtgccaccgt
     9781 caccataacc gtgcagaaca tcctcgacca actttgccct ggtttgctgg cgctcggtct
     9841 gacgctgctg atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     9901 cctgtttggt ttagggatta tcggtaatgc gctgggcttc ctgtcctgat tcttcgcccc
     9961 ggcacgactg ccggggtcat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
    10021 tctttatggc aaacgtgacc tgcgtctgga aacctttgac cttcctgaaa tgcaggagga
    10081 tgaaatcctc gcgacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
    10141 tctgggtgaa aaccataaaa aagtacccga cgatgtggcg accaatccca tcatcatcgg
    10201 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
    10261 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
    10321 ctactccttc ccgtgggtag gcggcgaagc gacgcatgtg gttattccca acgaggtcat
    10381 ggaacaagat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cgctggttga
    10441 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
    10501 taaccacacg atggggattc gcccgcaagg gcgcacgctg atcctcggcg gcaccggacc
    10561 aatgggactg ttggcgattg attatgcgct acatggaccc gttaaccctt cgctgctcgt
    10621 cattaccgat accgacaacg acaaattgag ttatgcacgc aagcactatc catcagaacc
    10681 gcaaacactg attcattatc tcaatgccgc cgatgcagca tttgatacgt taatggcact
    10741 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
    10801 tctcgcctct tccttgctgc cgacagatgg ttgtctgaat ttcttcgccg gaccgcagga
    10861 taagcacttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
    10921 gggcacatca ggcggcaata ccgacgacat gcgcgcagcg gtcaaattga ttgaagagaa
    10981 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
    11041 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
    11101 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcaattc tggcgcgtca
    11161 tcaggggatc tggtccggtg aggcggagca atacctgctc gcccatgcag aggcaatttc
    11221 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgccgc ggcaacttcg
    11281 cgacctgatg tgcggctgat tgcagaagtg gcacaatgtg cacgagaagg ataaaaaaac
    11341 atcagcgcct taagaggtgc tcaaattggt ggcaaagtgc acattgttta tgccggatgc
    11401 ggaatgaagc cttattcggc ctacataatc ttgaaaattc aatatattgc agttatctgg
    11461 taggccaggt aagcgtagcg catcaggcaa tcctgtgttt gtcatcagtc tcagcgcctg
    11521 tgcaatatgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcattc
    11581 cttccgtgtt ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
    11641 gctgccgggt aaagcctggc tggaacacgc gctgccgctg attgctgaac agttgcaggg
    11701 gcgccgttct gcggtattta ttcctttcgc tggcgtaacg cagacctggg atgattacac
    11761 agcgaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
    11821 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
    11881 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gcacccatta ctgacgtggt
    11941 gaaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
    12001 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
    12061 cccgctacaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
    12121 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
    12181 gccggaaggt aactggatca cagtaagtaa aggtcacgct acgctgggtg gcccgaacac
    12241 cacttatgtg tttaaggctg gtgaagaagc ggtgccgctg gaagcgggtc accgttttta
    12301 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgctttac
    12361 gcgtcttatc aggccttcag ggtcacggta ttaatactcg tcccgtcctt catcttcatc
    12421 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcagac gctgcatatc
    12481 tcccagtaag cctaaatgta gcgaactggt ttcaatactc tgcacgtttt gctggtgcag
    12541 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
    12601 gcgcaaacga cgggcgctgg taacagcgcc agagaagaaa accgacattg ccagttttaa
    12661 attactgagc aattgctcat agagcgcatc cagttccttc aagccatcaa gcgaaaatgc
    12721 tcgccttgct gccagcgatt tgtcggcaat ttcactcccc atgcgctcga cgatatcgga
    12781 ggcctgttca aggttgagcg acatttcgat aatctccgcc cagcggcgcg actcttcttc
    12841 tgccagttcc tcttttggca tccgcgccag atacagctta atggcggtat agagaacgtt
    12901 gatgtcatca gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
    12961 attcaaccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgggcagc
    13021 gttcgccaga gcaagcgtgg gcgtatccag cgcgctgaca tccagatgtt taggccgtag
    13081 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
    13141 aacgaatggc aacatgacca ggcagcgtac aaggttgtag aagacgtgga aatagatcac
    13201 caattccgct tttggcaatg gcaacttccc cattgtctct gccagcaaat gaacaaacgg
    13261 caggataatc aggctaccca ccagcttaaa cagcaggcta cccagcgcga cacggcgggc
    13321 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggtttgcacc
    13381 aatcaccaga caaagcgcca ctgggaaaga gataatgcct gcggcggtca gagtcgcggt
    13441 cagcagtacc gcggcaaggc tggagtagct gataatggcg aacatcgcac caatcagcgc
    13501 atccagcaaa atgtcgccag tcagcgaggc aaagatcacc tgaacgccgt ttgcctgagt
    13561 gatcggcgtt acggcctgca caatcaactc cagcgccagt aaaatcagcc caagaccaat
    13621 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
    13681 gataaaaata agtaacggtg acagccagga taaatcgaag gtgagaatac gcgccatcag
    13741 cgccgtcccg acatctgcac ccagcacaat caccagcgca ggcgcgagag caaccagatc
    13801 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgca ccagtgcggt
    13861 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
    13921 acgcagacgc gcgccgaaga cgcgcattac gcctgttcga acaatatgag tcccccagac
    13981 cagcagggcg acggcagaaa gcaggtgaag caaagttagc acggaatcag gttctccttt
    14041 ttgttatgca ttttcctgtt aagatttttc taatcacgtt gtttttatcc aggcatttct
    14101 cttgtatgta ctttcagtat aagagattta tacaagaaag catttaaaaa ggcatttata
    14161 taaaaatcca cctgtatctg ttgctatttt tgtcacactt tcccctgtcg tttacaccag
    14221 ataatcgttg tcacttttgt gacctctgag aagataaaca acgaaatatt taagaccaga
    14281 tcctgtgaat aaaaataatt aagtaacaac ggtattacgg agattgaaca gcttacggta
    14341 gcaaaacgca taaactcctg aatcgccaaa aaagagaaac taaataattt ttgtcttaaa
    14401 aaacattgcg c
//
LOCUS       DADTFG010000126        14356 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.127, whole genome shotgun sequence.
ACCESSION   DADTFG010000126 DADTFG010000000
VERSION     DADTFG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.127"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(161..490)
                     /locus_tag="J1138_003529"
     CDS             complement(161..490)
                     /locus_tag="J1138_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455285.1"
                     /translation="MKTFRWKVKPGMEVTSQPSVMEVRFGDGYSQRAPAGLNADLKIY
                     NVSLSVSRDDARRLEDFLAEHGGWKAFLWTPPYTYRQIKVTCAKWTTRINMLRVEFSA
                     EFKQVVS"
     gene            complement(487..3069)
                     /locus_tag="J1138_003530"
     CDS             complement(487..3069)
                     /locus_tag="J1138_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA8455286.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMTRVRRHFSGLETDARKTAGAV
                     EQSLNRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQSPWLILLQQGGQVKD
                     SFGGLIPMFRSLAGAVTLPAVGITSLVAATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANEAV
                     TKGFDEQTRRLKANMGTLETWADKVGSAFKSMWDAVLDIGRPESSAEMLNKAQQAFDE
                     ADKKWQWYQSRSNRRGKTSAYLSNLRGAWDDRENARLGLSAATLQADLEKASEMAARD
                     RAESEASRLKYTEEAQKAYERLQTPLDKYTARQKELNKALKDGKILQADYNTLMASAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGAHEKISQQRRDL
                     WKAESQFAVLAEAAQRRQLSVQEKSLLAHKDETLEYKRQLADLGDKVEHQKRLNELAQ
                     QAVRFEQQQSAKQAAIRAKARGLTDRQAKRESEEQRLRDVYRDNPQALAQVTGALKQT
                     WADEDMLRGDWLAGFKSGWGEWVESATDSFSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILIKQAMVGIVGSIGSAIGGAIGGAGASASTGTAIQAAAANFHF
                     ATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYADGGYVGGSGSQA
                     QMRRADGISFNQNNNVVIQNDATNGSIGPQALALVYDMARKGARDEIQAQMRDGGIFS
                     GGGR"
     gene            complement(3050..3463)
                     /locus_tag="J1138_003531"
     CDS             complement(3050..3463)
                     /locus_tag="J1138_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA8455287.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDAQLDMHFSGLTY
                     AVLSLFFCDPEMHPSDFSLLVPRREEEQTEMPDEEEILMQKAAGLAGGVRFGGEGEGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            complement(3490..3918)
                     /locus_tag="J1138_003532"
     CDS             complement(3490..3918)
                     /locus_tag="J1138_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA8455288.1"
                     /translation="MFLKTEQFEYNGVTLTLSELSALQRIEHLALLKQQEEQAAASGN
                     PQVSVEELVRTGAFLVAMSLWHNHPQKTQMPSMNEAVMKIEQEVLTTWPADAIARAEN
                     VVLRLSGMTGPVRMESDFTEEAENVDTDVSYSAGKPSTAS"
     gene            complement(3934..4683)
                     /locus_tag="J1138_003533"
     CDS             complement(3934..4683)
                     /locus_tag="J1138_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455289.1"
                     /translation="MATPNPLVPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEINVTDGAAG"
     gene            complement(4691..5086)
                     /locus_tag="J1138_003534"
     CDS             complement(4691..5086)
                     /locus_tag="J1138_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455290.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMERRIYPAMAAIPALAGMITTM
                     VQQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(5083..5616)
                     /locus_tag="J1138_003535"
     CDS             complement(5083..5616)
                     /locus_tag="J1138_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455291.1"
                     /translation="MAIKGLDQAIENLSRIRKSAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERARLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKSGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKKRYPIDVVKIPMAVPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
     gene            complement(5632..5985)
                     /locus_tag="J1138_003536"
     CDS             complement(5632..5985)
                     /locus_tag="J1138_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455292.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGTQRGGEVHGVFD
                     DPESLGFTGSGVRIEGSSPSLFVRTDMVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPVVNRRR"
     gene            complement(5978..6361)
                     /locus_tag="J1138_003537"
     CDS             complement(5978..6361)
                     /locus_tag="J1138_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBA8455293.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMYGDESGAEKNYTEDAGQLNNTDDPDDVKAVRVRKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSPLATAMIQHGTADHA"
     gene            complement(6413..7441)
                     /locus_tag="J1138_003538"
     CDS             complement(6413..7441)
                     /locus_tag="J1138_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBA8455294.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRMVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGIVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(7499..7846)
                     /locus_tag="J1138_003539"
     CDS             complement(7499..7846)
                     /locus_tag="J1138_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBA8455295.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIHWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(7883..9388)
                     /locus_tag="J1138_003540"
     CDS             complement(7883..9388)
                     /locus_tag="J1138_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBA8455296.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEVQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(9378..10970)
                     /locus_tag="J1138_003541"
     CDS             complement(9378..10970)
                     /locus_tag="J1138_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA8455297.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGYGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQEQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            complement(10967..11173)
                     /locus_tag="J1138_003542"
     CDS             complement(10967..11173)
                     /locus_tag="J1138_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-stabilizing protein"
                     /protein_id="HBA8455298.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRCRRRPLGVRL"
     gene            complement(11157..13085)
                     /locus_tag="J1138_003543"
     CDS             complement(11157..13085)
                     /locus_tag="J1138_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA8455299.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVLPMTAVEWADQYYYLPKES
                     SYGDGEWKTLPFQVAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVSCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDKPESVFYLCEH
                     HGCVIHQSEPDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVDEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDPAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            complement(13057..13566)
                     /locus_tag="J1138_003544"
     CDS             complement(13057..13566)
                     /locus_tag="J1138_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBA8455300.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
BASE COUNT         3160 a         4446 c         3347 g         3403 t
ORIGIN      
        1 taaaatagct gcccattcaa tattcaaaat gtaagagaat gtaaaaaaaa gatccagaca
       61 acactttata ataagaaatg agacaaataa atatataaaa tagcaattta ttattttaat
      121 tattttcact acttttattt attttattat tttatccact tcagcttact acctgtttaa
      181 actcagcact aaattccaca cgcagcatat ttatcctggt ggtccattta gcacacgtca
      241 cctttatctg cctgtacgta taaggcggcg tccacagaaa cgccttccag cccccgtgct
      301 ctgccagaaa atcttccagc cgtctggcat catcacggga cacagacagc gaaacgttgt
      361 agattttcag atccgcattc agcccggcag gcgcacgctg ggaatacccg tccccaaaac
      421 gcacttccat aaccgacggt tgagaggtca cttccattcc cggtttcact ttccagcgaa
      481 atgttttcaa cgcccacctc cggagaatat accaccatca cgcatctgtg cctgaatctc
      541 atcacgggct cccttacggg ccatgtcata aaccagcgcc agtgcctgcg gaccgatact
      601 gccgttcgtg gcatcattct gaatcaccac attgttgttc tgattaaaac ttatgccatc
      661 tgcccgccgc atctgagcct ggcttccgga accacccaca taaccgccat ccgcataccc
      721 ccgcatcagg cggtacaggt tgccgacacc aatccggctg gttgcctcct tcgtgaacac
      781 aaactcacca cggtgaacaa tccccgcagg ctcatatttt ccgccagtgc cggtgaatcc
      841 accggtcgcg aaatgaaagt tcgctgccgc agcctgaatg gctgtccccg tggaagctga
      901 tgcgccagca ccaccaatgg ctcccccgat ggcgctgccg atactcccga caatccccac
      961 catggcctgt ttaatcagga tttccgtcat catggacagc accgaacggg tgaaactgcg
     1021 ccagttctgt tcactgccgg tcagcattgc cgccatattc tgtgcaatac catcaaaggt
     1081 ctgcgttgca gcacttttaa cctgcgaaaa actgtccgtc gcactttcca cccactcacc
     1141 ccagccagac ttaaacccgg ccagccagtc accgcgcagc atgtcttcat ccgcccatgt
     1201 ctgtttcagt gccccggtga cctgtgccag cgcctgcgga ttatcgcggt acacgtcacg
     1261 aagacgctgc tcttcagact cacgcttcgc ctgacgatcg gtgagaccgc gggcttttgc
     1321 tctgattgct gcctgcttcg cgctctgctg ctgttcaaac cgcaccgcct gctgtgccag
     1381 ctcattcagg cgtttctggt gttcaacctt gtcgcccagg tcagccagct ggcgtttgta
     1441 ttccagcgtc tcatctttat gcgccagcag ggatttctcc tgtacggaca gctggcgacg
     1501 ttgtgccgcc tccgccagta ccgcgaactg actttccgcc ttccataaat cccggcgctg
     1561 ctggctgatt ttctcatgcg caccgctgtg tttttccagc gtccggagct cggtttcaag
     1621 cgccagcagg gcagcatgcg cctggtcttc ctgacgttca ccggctgaca cttttacacc
     1681 tgacgacttc ggttttttca gcgtcgactc atagtccttt ttcgccgacg ccatcagcgt
     1741 gttgtaatct gcctgcagga ttttcccgtc tttcagtgcc ttattcagtt ccttctggcg
     1801 ggcggtatat ttatccagcg gcgtctgcag gcgttcatat gccttctgcg cctcttcggt
     1861 gtacttcagc cgtgaggcct ctgattcagc ccgatcccgt gccgccattt cgctggcttt
     1921 ttccaggtct gcctgcagtg tcgccgctga aagaccgagg cgggcgtttt cgcggtcgtc
     1981 ccatgcgccg cgcagattag aaagatacgc tgacgttttt ccgcgccggt tactgcgact
     2041 ctgataccat tgccattttt tatccgcctc atcgaatgcc tgctgtgcct tattcagcat
     2101 ttctgcagat gactcaggac gcccgatatc cagcactgca tcccacatgg atttaaacgc
     2161 actgccgacc ttatccgccc aggtctccag cgttcccatg ttcgctttca ggcggcgggt
     2221 ctgctcatca aacccttttg tgacggcctc gtttgcggcc tgcaatgccc cggcctcatc
     2281 acccgaacgc tgcagttgcg caacatacgc aatctgctct gccgtcacgt tacggaactg
     2341 gcgcgccatc gccatcagcc ccgacgtcgg gtcggtggtc agttttccga aagcctctgc
     2401 aaccttgtcc acttccacac cggatgcaga agcaaaacgc gccacactct gattaatggc
     2461 atcaaactgt tcaccaccac gcacaccggc gttcaccagt gctgccagtg actcactcgc
     2521 ctgattaaac gtcagccctg ctgcctgtcc tgctctggag agagtcagca tgcgatctgc
     2581 ggtcagcccg gactgattac cggaaagaac cagggtttta ttaaacgctg aaagcgtgga
     2641 gtccccctgg taccaggcat acgccagcgc cccggttgct gcaacgagtg atgtgatccc
     2701 gacagcaggt agcgtgacgg caccagcaag gctccggaac atggggatca gcccgccgaa
     2761 ggagtccttc acctgtccgc cctgctgcag caggatcaac cacggactct gaccaccggc
     2821 aagctgcgtg gcgatatccg tgaactgtgc gggcagggta cgcatggcgg ctttatactg
     2881 cccgacggaa atcccggctt tttgtgcggc cagcgcctga cggttcagac tctgctcaac
     2941 ggcaccggcg gtttttctgg cgtcagtctc cagtcctgaa aaatgacgcc ttacgcgggt
     3001 catctgctca tcgaaacgga ccgcatccag actcaggtca ataacaagat caccaaccgg
     3061 ctgggacata tctcacacct ccggaaatcc ccgctgaagc catcattaat gcaacatcat
     3121 cctcgctgac atccaccaca tccgcagaag gtgaaatatc gccctcaccc tccccaccaa
     3181 accggacgcc tccggcaagt cctgccgctt tctgcatcag aatttcttcc tcatccggca
     3241 tctccgtctg ctcttcctct cgccggggga caagcagact gaaatccgag ggatgcattt
     3301 ctggatcgca aaaaaacagg ctgagtacgg cgtacgtcag cccggaaaaa tgcatatcca
     3361 gttgggcatc ctgaaaataa tgcgtgcggt aaaaacgtcg ccagtcggca tattcggtgg
     3421 atgtcatccc ggcaagcatg gcgcgccagt caggcctccc catctcacgc gccagtctga
     3481 gggcaaaatt cagctcgccg tcgaaggctt tcccgcggaa taagaaacgt cagtatcaac
     3541 attttctgcc tcttccgtga aatcactttc catccggaca ggcccggtca tcccggacag
     3601 acgcaatacc acattttcag cccgggcaat ggcatcggca ggccaggtgg tgagcacttc
     3661 ctgctcaatc ttcatcacgg cctcattcat ggacggcatc tgcgttttct gtggatggtt
     3721 atgccacagg gacatcgcca ccagaaaagc gccggttctg accagctctt ccacgctcac
     3781 ctgcgggttg ccactggcgg cggcctgttc ttcctgctgt ttcagcaaag ccagatgctc
     3841 aatacgctgc agcgcggaca gctccgacag cgtgagggtc acaccgttat attcaaattg
     3901 ttctgttttc agaaacatgc tttatctctc acctcagcca gcggcaccat ccgtgacgtt
     3961 aatttcagca actgcagcaa actcaccatt gccggtgaca acagggatct gcgctttacc
     4021 tgccgcaaca cctttcaccg tgatcgtgtt ccctttcacg gtaatggtcg cgaaattctg
     4081 attcgctgac gtggcgcgga acgttttatc cgttgcgcct tccggctgaa cggccacggt
     4141 cagggtgata ttctgacctt ttgccacatt gcccgttggt ggcgtcacgg taataccggt
     4201 gaccggtgtg atgtcccccc gatcctccgc cagcgaaggg cgaccgatat tggtaatttt
     4261 taccgtacgg gtgatcacct ctttggcggt caccgcttta ccaatggcgc tcacccagcc
     4321 acggaacaca tccaccgtac cgttcgggaa gcggattttg taggcccggg tctcgctgct
     4381 gtcaaaccag gcaatcaaat cacgctgccc tttctcgccg ggcttccagg ccagcgtaaa
     4441 actggtgtca cctgcagatt tctgcccctg cccggtggat acccagtcag catcctcatc
     4501 atccagatag ttatcgtcgt aggattctgc cgtcatctcg ccgggggtca gatcctttat
     4561 ttttgccagg cgagtccact catcatctga cagcgggttt gcataagcat cgcccttacc
     4621 ggtgtaaacc cagagcgtgg tgccggaacc ttttaccggc accaggggat ttggtgttgc
     4681 catatcatcc tcacatttcg taggtaatgg aatacgtcag atcagcagaa ctccataacg
     4741 ccatatcgtc atcgcgacga tactcatagc cctgctgcac catcgtggta atcatgcccg
     4801 ccagtgcagg gatcgccgcc atcgccgggt aaatccgcct ttccatccac tgatcaagct
     4861 ctgaatccgg tacctgtgcc ggtaaaaaca cctcaatatg cagtgtggcc cgccaggtat
     4921 ctgcatccag ctcttcaccg gtatactctg catccgtcag ataaaccgcg accgccggaa
     4981 aatccgcctc ctcaaaaaca gcggggcgac catcaaacag cgtcgccccg gtgtcatgct
     5041 tctccagtgc atccagcact gccgcacgga tatcagtatg tttcatcgtt ttatcgcaat
     5101 cctcagttgt tgtttcagcg catatgccag ttctttgggc aggcgttcac gccggatacg
     5161 gtcaacattc tcatcaaaag cctgtttcag tgggaccgcc atcgggattt tcaccacatc
     5221 aatggggtaa cgttttttcc cggccacacg ctgcatgaca tgccagcgac cgttttttaa
     5281 tcgctggata aatgcccgct gataacgatg ctgaccggat ttgagtatgc tgttcggacg
     5341 acgccccagc attctgatcc ccagcttaat cacaggaaga tcaccgcggt taacgataat
     5401 ttttgcattc ggatttctga ccgtagcccg tttcagtctg gcacgctctt taaccagttt
     5461 ccggctcacc cgggtctccc gggcgacctg tgacgaagac tgattaatcg ccgttgtggc
     5521 cacgcggtta atggccattg ctgaagcagc cggaatggcg cttttacgaa tccggctcag
     5581 attttcaatc gcctgatcaa gcccttttat cgccatcatt tccccctgcg tttatcgtcg
     5641 ccggttaact acgggtggtt gcccacggtt gagccagaga taacagctgc ccccgtcatc
     5701 cggagaaaca cgatccaccc agaacatctc gccgttaatg gtcagcgtgt caccacgccg
     5761 cacagcacga accatatccg tccgcacaaa taatgacggg ctgcttcctt caatacggac
     5821 cccactgccg gtaaaaccca gcgactccgg atcgtcaaaa acgccatgaa cttccccgcc
     5881 acgctgtgta ccggaggtga actgcgcact gatacccatc acttcaacaa ttgtgctgtc
     5941 caccccggca agagcggcat caaaggcatt ctgaaagtca cgcatgatca gccgttccgt
     6001 gctgtatcat ggctgtcgcc agtggtgatg gcaccagaac acgcatcccc cggaacgtca
     6061 gctcaacagg acggcctgtc tccgggcaat accccatcac atgcaggcac ttgcgcacac
     6121 ggacagcttt aacatcatcc ggatcatcag tgttgttcaa ctgcccggca tcctctgtgt
     6181 aatttttctc agccccgctc tcatcgccgt acataatgcc ctcccgggaa gcagcaagct
     6241 cctcttccca ctcagacaca cgttgcgcaa tatccgcagc actccccgac atatccgcct
     6301 cgcgccccag caggccagcc agttgacgaa gacgattcag attttcttct tttgttgcca
     6361 tctcagtctc ctgtgaataa agacacgggg gcatttcgcc cccgctcacg gattatttca
     6421 cctgtaccac cacaaactca tccgggtccg gcaacaccat cagcggcgca gactgcgtca
     6481 tggtaaattc acgggccgga tcgcccacgg tcagccagtg tttcggataa cgggaagagg
     6541 ccaccacacc ctcagacaac gcctgcgcat cctgaatggc accatagcaa cgaatgccct
     6601 ctgctgccgt attccccagg accagtgtgc cctccggcag ataacgtttt tcggtaccgt
     6661 cctctgccac ataagacgtt ttcgccacca caatggccag atcgccgtaa taccccttga
     6721 aggacaccac tgcgcccaga tctttcactg ccgtttcgag ttgtgaattt gagccgcgac
     6781 gggtatccag tttttcgcgg aacagtttaa agccattcag cagacgccag acgataccgt
     6841 ccatgatggc aatattcaca agaccgctgg cctgatcgca gtagaggtca atatcatgcg
     6901 tcggatcaaa cgtatcacgg tcctgctcag accatttttt accgtcagcc tgctcaatgt
     6961 tatttccttc agagcgcccg aaatccacct cgacagtatc aaactgctcc ccttccatcg
     7021 tgtatttgcc atacagcacg gcattcaccg cctgcatttc ttccacctgg acaatcgcgt
     7081 gctcttcctg tttgaggtta tcggtaatga tacgcagacg gcggtaggcc gggtcgttca
     7141 gctgagccgg atcttcaccg ggaagacgct caaccgcctg ctggtaatta aattcgtgtt
     7201 tcggcttgac gtagcccgga cgtaacacgc gggtttcacc accacgatga cgcagcactt
     7261 ttccttcaac aaccggggag acataggccg ccaccggcgt ttttccggta attttgtcca
     7321 gcatcacttc ttcggtatgg aaattcacca tacggcggaa aaacagctcc agaaacagcg
     7381 cacgaaattt aactttttgt tcggtataac cgagtaactg gcgggtcgta aacaatccca
     7441 taaatcagtt cctttcattc agaaatcagt caggccaccg cggtggcctg ataacgtgtt
     7501 acggcagcgc cgcgtgactc agggcactgc cggcaaaggc gttggccttt ttgtgttcat
     7561 ccacactttc aggccagtgg attgcctccg tcgcaaaggt tcccgacttg taatacgtca
     7621 gcgccgtctc tgtgccttca agcggcagta ccagtatgcc aaccgcacta ccggctttct
     7681 gtccgtccca gaccaccagt ttcccggtgg cttcatccag catcaggggc gtcagtgccg
     7741 gtgttgccga ggaaatcccg ctgctgcctg tggcggtatg agccggatca ttaccggcaa
     7801 aaatacgtac ttccgcacgc tgttcagtga tggttttcgt caccatattg taaaaacctc
     7861 ctgttgatgg tcagcactgg cttcatggca tggccatgag cattttcacg tccgcatcac
     7921 cgtctgctga cgtctgtggc acgccaccct gtaccgctgc cggtgaatgg ttcgccatga
     7981 aatgttcaaa catggcggtt gtggatgcag agaccggttc tgccttacct gatcccgcag
     8041 ccagcacagc ccgggcgctc tccacagtca ttcccgggca ggcagccagc tgttcagcct
     8101 gcacctcagc cccttttgcc tcatccagtg ccatgatctg atcacggagt gatggtccgg
     8161 catccgcctg cggtgaagca gccaggatcg ggcgggcttt ttccaccgtc atctccggca
     8221 tcgccgccag cgttgccgcc agttgttcac gaccgttcgc ttcttcacac gccataatgc
     8281 gatcggcttc actctgcgtg gatgccaccg gctgctgcgg tgccgccgcg gccagaatcg
     8341 cccgggcctg ttcaacgctc atgccctgtt gtcctgccag catcgtggca agctgttcac
     8401 gtcctttcgc ttcctggcat gtcaggatcc ccatcactcg ctggttctcc tgcgcggcgg
     8461 cttccgttgc agttaattgc ggcatagtgc ctcctctgac attactgttc agcgccgtgg
     8521 ccatcacact gatggcatcc gacgcattga ttaattcatc cgccagcccg gcctcaatgc
     8581 cggactgacc ttcaaaaaca gcggcctctg ttcccgtgac tgcatcaaca gacagaccgg
     8641 taaacatcgc cactttttcg gcaaacatcc ggcgcgccgc atcaatccgc tgctgcatgt
     8701 cctggcgaac ctctgccggc aacgcttcaa actgattgcc atccaccttg tgcgcccctg
     8761 agtaaatcag cgtgatatcc acaccggcct gcgccagatg accggcatag ctgacatggc
     8821 tcatcatcac gccaatggaa ccgatacggg atgtctgggt aaccagccgt cgggagcagg
     8881 ccgacgccag cagcatggct gcagaacagg ccgtgtcatt gcacagtgcc cagaccggct
     8941 tctgctgacg gaggcggtaa atcatgtcag cgcagtcaaa cgcgccggcg gcctgcccgc
     9001 ccggactgtc aatgtccagc agtacgcccc gcacctggct atccgccatt gcctgctgaa
     9061 gacaggcgac aataccgtca tagcctgtca ttccggaaaa tggccgcata ccacccagcc
     9121 ggtgcaccag cgtgccggtc accggcagta cagcaatacc gttcaccacc ctgtaaacac
     9181 gggccggtcg tttacctccg gccatgtact cgtccgtttc agccagcatt ccgggagcat
     9241 caaactgtac ctgctgttgt ggtaccgaaa gacttgctgc ccccatctcg cgcccgagcg
     9301 cgcaaaagaa aacccgcgca taggcgggct ccagaagcag cggttcattg aatgctgcgg
     9361 caataatgtg tgaaagatta cgtctcacgt ggtgttgtct cctcttccgg cctgcgactc
     9421 tccgctatct gctgctgata cgcctgcgct atccacaccg gacgtgagag tccggctttt
     9481 tgccgctcag cagattccct gacctgctgg cggaaaatgt cctgataatc ctcgcccatc
     9541 agcgccagct ctttctcata cgtgctcagt ccggcctcaa tgcgcatcac tgattcctgg
     9601 acttccttga gcccgtcaat ggccattctt ccggcaccaa tccactctgc ccgtgaccag
     9661 gctgatcgcg cctgataaaa atcaaaacgt gcccgtggcg gacgaataat cccccgaaga
     9721 agtgcctctt ccagccagca ggaaaacatc tgcgtggcca gccgggccgc aataaatttt
     9781 cgccgcccca taaaatagcg ccacgactca ttggcggagg cgcgggcact tgagtaactg
     9841 accttcgagt aatcacggga caactgttcg taggaaacgc caagaccggc ggcgatatac
     9901 cgcagcagcg cctgttcaag cgcagaaaat ccattgtctg aatcctgcgc agtctgtagt
     9961 ttcagatcat caccagggaa aaggtgcgga attttgacac cgcccagtgt cacgttattc
    10021 gtgtcatacc agctggaaaa cttctccaga atattaataa gcggattatc cttctgctcc
    10081 tgtggcgcgc cggcgatata ttcaaaggcc ttttcggtat caagttcact ttcaatcgtc
    10141 gctgcataca tcgccttcac tatggccgac tgaagctgtg ttgcctgcag ggaatcgagc
    10201 atcttcagcc gttccatgac gctgtaaaac tgattagccc cacgggtctg cccgtcctcc
    10261 accggctcga aaatatgcag catggccgga cgcccggtgg gaagttcacg cgggatccgt
    10321 tcccatcgtc cactaccaga gaacggaaaa tcatcctcac agatatggta cgcgacggca
    10381 cggccatatc gatcgacctc cacaccggcc cgcagaaaac ggttccccat accgtatcca
    10441 ggcgtgtcca cccgtttcgg actcacggct ttaaaacgcg tacggaataa ctgcgtggtt
    10501 tccgtatccc agaccggctg cacaaagatt tcgccgttaa acgcatgaac gcccacacct
    10561 tcacggataa attccgtgaa cgtgcgtttt ccttccacgt cgatctcgcc aaacatccct
    10621 tcggcgtatt ccgaccaggc cgcctccacc tcatcgacaa agctttttgc tgcggtctcc
    10681 cgcatcccca gccagcgcca gttcggacgg tagctgatca gaaacatatg cccgacaatg
    10741 tgatccttat gcagagccac cgcattagcc gctattccgt tattgcgcac cagatcatct
    10801 gcccgggcat tccccagacg caacgcgggc agcagggccg catcggcact ctgcgccggt
    10861 ggcaaccact cagccatttg cccgccaaat cctgcaccgc ccccgttgta gctgagactc
    10921 tcacgaagcg gaacgccgtt cacatcaatc aggacaggcg ttcgtttcat aatctcactc
    10981 ccagcggacg acggcgacac cgggttgtcc ccagtaccga ctccgcatca ttgatcgccc
    11041 ggttaagctc atccagagaa gccgccgtat attcaattct gcgaccatct ttctggacag
    11101 acaccacccg tttaccggtt aataaatcaa ggcgcgcctg acgcagcgcc tgcagttcag
    11161 cgactgtaac cattcactcc tccggacagc ttcgctgcca gttctttaag agttggccgg
    11221 gtcgtctctt cttcccggga ttttgccagt acagccggat caagctgcca gcgttgcacg
    11281 gacacacgta atgccgcgta ggcatacacc aggcagtcca gcgcttcgtt acgccgcttt
    11341 ttgttatccc acagcagacg catctttcct ttttcccact tctccacaag ctcttccgcg
    11401 accagttgct gcgcctctgt ctgcgaaaaa atctccggat catcaggaaa acggatggca
    11461 tacgacgtgg cttcatccac aggcgtggga tcggctttca tacgggcata gagaatttct
    11521 tttgcggtgt ccgtccccac ttcacacaga tacacgcccc gctgattgcg ggtttttggc
    11581 atggtgatca ccggcttgcc atagacagat gcgcctttta ccggcagcac ccggaaaaca
    11641 ccgtgttttt ttgacctctg ataaacgatt tcgccatcga tccccccgat gtcccagcag
    11701 acacgggaaa tagtcatttc ggttccgtct gcatgacggt attttttgtt gatcgccgca
    11761 tccacacgta acagcgtctc ttcctcatcg ggacgcccca taatgatgat tttatccacc
    11821 agaaaggctt cctctcccgg agcccatccc cagacataca tctcaaaacg gttacgctgc
    11881 gagtcaatgc ccgccgtcag ataaaccacc cgggcaggca ctgcagccgt gtaacgcaca
    11941 accttatcca tcagtacctg gtgatcgagt ttttcgccca cggcctcttc ccaggtctcg
    12001 cccagcgtgg tgttcacaaa ggttttcacg ccattgggat ctttcagtgc atccagccag
    12061 tcatagacta tctgtaccca ggtggtgaac ggactgtacg ccgtccagat atggaacgtg
    12121 atggagcgcg gcggcggaat ttcatcaccc cgggcgctga aaaacatcag gccgtcacgg
    12181 gtccacatgc ccgtgttttc acagatccag cgcccgttgc tctggtcagg ctcagactga
    12241 tggatcacgc agccatgatg ttcacagagg tagaaaacgc tttcaggttt atccttctcc
    12301 catttgaggc caaaaggcgt ggactcatcg ccaaatttca gatactgcgc ctccccacag
    12361 tgcgggcagg gcacataaaa acgcatgaaa tgtgccgact cgttggccgc tttttcgatc
    12421 tggcaggtgc ctttgatttt aggcgtcgag ccgcgaatgg attttggcca tacagagccc
    12481 tcaatacgtt tatcccccag cagggttggc gaaccctctt tttcgacatc cggttcgaac
    12541 gaggaaagct cgtcataaca gaccacatcc acggattttt cacggtagtt tttggcggca
    12601 gcaccaccca gacaccagaa ccccacaccg gaggaaaagc gtttcagcgt gagggtattg
    12661 tcacgatgtt tacgtcccag ccagggcgac agttttttca ggcaggaaac atcgcgaatc
    12721 gtcgcctcca catgagactt cataaaatct tcagcggcag aatccgtggg ctgaaaaagc
    12781 aggctgtttc gggatttatg ctcaataaaa tacccgacca cccccagcaa catctttgta
    12841 tagccaacac gggcagattt aatcaggtta accgtgcgaa cctggtcgtt acccatacag
    12901 ttcataatgg cgacctggaa cggcagcgtt ttccattcgc cgtcaccgta tgaagactct
    12961 ttaggcagat aataatactg gtcagcccat tcaactgccg tcatcggcaa taccctgacc
    13021 agtggttgca gcgcaaccga aacggcagcc atcatattat tcagttgttg ctctgatata
    13081 ttcatcgagt aaatccggta atttatcccc tgcacgcgca cactgatttg ccccctttgc
    13141 aataagggtt ttcagatggt caagatgacg tggcgttaaa tccgggaact gtcgctgcat
    13201 ggataacgga atggaatcaa gcgtactgga taacgccatt gccagcttac tgagggcaaa
    13261 aatacaaaat ccggtgtcga tcagttttcc ttttgacacc tcatttttta gctgttgtgt
    13321 aacggcctgt tctgctgtca gttcccatct ggcaataagc aatttctctt catagtcttc
    13381 ttcgctatcg ccatcaggca catcgttttt acttctcctc agatacgata tgtaaaaatc
    13441 acgccaggca tccagatcca gttgccctcg cttattcgag accggggcac ccggtaattt
    13501 ctgcaatctg cgaagctggc gatcggtcag actcaaatgc ctggcaactt cagtctgcgt
    13561 agccactcct cacacctcgc aaaaactctc acctcacaat cacgacaaaa ccggtcatgt
    13621 ccggtttaca tgcccatttt ttgcgcatgt ccggttcaca gaagacctgt ttttatattt
    13681 ttcatatagt taacctacag agaaaccgga catggatccc ggaaaatttt cataaatagc
    13741 gaaaacccgc gaggtcgccg ccccgtaacc tgtcggatcg ccggaaagga cccgttaaaa
    13801 tgataacgat tatcaataat gcctgattcc attctctctg gtgatcatgc cggacgggtg
    13861 attctgaatc cgccctctcg catggctgtc agcatggata atgcgaaaat acaaaacatg
    13921 attaattcac tgtactcagg ttaagcttca tgggtgcatc ccatactgac atatgattaa
    13981 actgcaccat attgctcttc tcctgtactg gtgcactgcc gcttttgtca tgttccctga
    14041 gggaaccttg agacactgga cagaaaaata atctgtactg actttacttt ttctgcactg
    14101 attgtgtttt ctgacattta gtgaccagag aaaaaatacg gtattgccat tcagttaaca
    14161 cgaatattcc gtgttgatta caataagtaa aataatataa aaacatggtc gcaaaaataa
    14221 accatctctt ctattcgtgc cattttccgc cgcataccac catgcggcat ttttttatca
    14281 tcctgccagt atctggatac ctgtttttta tttccatcat cgccataccg gcatgtgtat
    14341 gccctgctgt tgacga
//
LOCUS       DADTFG010000127        14084 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.128, whole genome shotgun sequence.
ACCESSION   DADTFG010000127 DADTFG010000000
VERSION     DADTFG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.128"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(138..1184)
                     /gene="aroH"
                     /locus_tag="J1138_003545"
     CDS             complement(138..1184)
                     /gene="aroH"
                     /locus_tag="J1138_003545"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBA8455301.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQPRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHAEDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1341..2174)
                     /gene="ppsR"
                     /locus_tag="J1138_003546"
     CDS             complement(1341..2174)
                     /gene="ppsR"
                     /locus_tag="J1138_003546"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBA8455302.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2507..4885
                     /gene="ppsA"
                     /locus_tag="J1138_003547"
     CDS             2507..4885
                     /gene="ppsA"
                     /locus_tag="J1138_003547"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBA8455303.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYGQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKQTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4942..6642)
                     /gene="fadK"
                     /locus_tag="J1138_003548"
     CDS             complement(4942..6642)
                     /gene="fadK"
                     /locus_tag="J1138_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416216.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBA8455304.1"
                     /translation="MHPTGPHLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKTGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSQILADKTPLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE
                     "
     gene            complement(6645..6938)
                     /locus_tag="J1138_003549"
     CDS             complement(6645..6938)
                     /locus_tag="J1138_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBA8455305.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(6935..8224)
                     /locus_tag="J1138_003550"
     CDS             complement(6935..8224)
                     /locus_tag="J1138_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA8455306.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLSDIAHAQKSVPQMLEDFKQHPAIRPLISSGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8279..9217)
                     /locus_tag="J1138_003551"
     CDS             complement(8279..9217)
                     /locus_tag="J1138_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBA8455307.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(9237..10001)
                     /locus_tag="J1138_003552"
     CDS             complement(9237..10001)
                     /locus_tag="J1138_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBA8455308.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGELLQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTKRKHIILDNDSPEAIAELAEHLKKALN"
     gene            10317..11228
                     /locus_tag="J1138_003553"
     CDS             10317..11228
                     /locus_tag="J1138_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA8455309.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(11271..12422)
                     /locus_tag="J1138_003554"
     CDS             complement(11271..12422)
                     /locus_tag="J1138_003554"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBA8455310.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(12436..14031)
                     /locus_tag="J1138_003555"
     CDS             complement(12436..14031)
                     /locus_tag="J1138_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBA8455311.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     ELIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT         3429 a         3566 c         3537 g         3552 t
ORIGIN      
        1 acgcataaaa attaataata atttatataa caatgattct tattatcaat attatggcgg
       61 gccagagcaa gcagtaattt gttgagaaga caacaagttg cgcagaaatg cccatcccgt
      121 caggaatggg cacgcattca gaagcgagta tctaccgcag aggcgagttt ttcgaccagg
      181 cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta agtgagcggc
      241 tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat aattccagca
      301 atcgccgtag agccattgcg gatttgctga caaatatcct cacaaacttc taactggcga
      361 cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc aggtaaatca
      421 aactcgtgca gcgtatcgca ggctgcggcg atatcctcgg catgataatt cggttttttg
      481 ccaccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat ggtcatctga
      541 ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat agcatccacg
      601 gcaatccgcg tgttgccatc ggtaccattt ttaaaaccca ccggacagga gagtgccgaa
      661 gccatttcgc ggtggatctg actttcggta gtacgtgcgc caatcgcgcc ccaactgatt
      721 aaatcagcaa taaactgacc ggtcaccata tcgaggaact cagtcgcggt agggacgccc
      781 agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt tacccgatag
      841 ctaccgttta aatctggatc ggagatgagt cctttccagc cgacaaccgt tcgtggtttt
      901 tcaaaatagg tgcgcattac gatttccagc cgtggctggt actggttgcg tagcgactgc
      961 agacgggtgg cgtactccat cgcagcggtg agatcgtgga tcgagcaggg gccaataatg
     1021 accaacagtc gcttatcttc accattcagt attttttcaa ttctacggcg ggagtcggtg
     1081 acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc gggcgttacc
     1141 aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag tctcagaaat
     1201 ttgttgcttc tacggcagga ctaccgggaa gtgatgcgca tcacgataaa ccaatcgcag
     1261 ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat aacaaaaaag
     1321 taagccaatg cactagttct ctagtacatt cggcggctaa ggcccatgat atcgaggatc
     1381 ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat ctgattttta
     1441 cggtacaacg cttccacttc cgcgacttcc atcctgcact gacgaagcga ggcatagcgg
     1501 ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg
     1561 aacaatttgt gctgaagtgg tttgagcgac gcgggcagca ccagattatc catatcgtcg
     1621 gcaataaagg ggtaatttgc cgcgcggata ccaaattgca ttgccagata cagactggtg
     1681 ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctggtc cagattgcgc
     1741 aacgaaatac cgtcatcgtg ggcaagggtg taatcaatcg ccgcaatgcg cgcatcatat
     1801 ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt tggatccagt
     1861 ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca aaagccttca
     1921 ctttgcaaga tgatagcgcg aatctccggc aacacgatgg agtagaagac cagcgggcgc
     1981 acacctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc acggctctca
     2041 ttttcgacaa acggcagcgt aatgctgctg atagtgacgg gaaattgtga cattactgcg
     2101 tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac gtggcgatca
     2161 acagcattat ccattttgtt cttcccgtga tgcagacata attctatgaa agcataaatt
     2221 aaaaacgcac agaagcgtag aacgttatgt ctggtttata aaatgaaact taaattttat
     2281 tttttatgaa aacagcattt catatttatg gtttcgttta taccgatggt ttatgtggaa
     2341 attgtcgaag agagcagatt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
     2401 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
     2461 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
     2521 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
     2581 gggcaaaaat gcctccctgg gtgaaatgat tactaacctt tccggaatgg gtgtttccgt
     2641 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
     2701 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta cccagcttgc
     2761 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
     2821 aaacgccatc cgcgaagcct atggacagct ttccgccgat gacgaaaacg cttcgtttgc
     2881 ggtgcgttcc tccgctactg cagaagatat gccggacgct tcttttgccg gtcagcagga
     2941 aactttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaagc atgtatttgc
     3001 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg accatcgtgg
     3061 cgtagcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
     3121 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
     3181 gggccttggt gaaatggtcg tgcagggtgc ggttaatccg gatgagtttt atgtgcataa
     3241 acagacactt gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
     3301 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
     3361 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
     3421 ggccgtacaa attgagaaac actacggtcg cccgatggat attgagtggg cgaaagatgg
     3481 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
     3541 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
     3601 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
     3661 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
     3721 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
     3781 cgctcgtgaa ctcggcattc cggcggtagt gggctgtggt gatgcaacag aacggatgaa
     3841 agacggcgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
     3901 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggacc tgccgttgaa
     3961 ggtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgaacga
     4021 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
     4081 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
     4141 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
     4201 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctttctgatt ttaaatcgaa
     4261 cgaatatgcc aatctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
     4321 cggcttccgt ggcgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcgctgga
     4381 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg actaacgttg agatcatgat
     4441 cccgttcgtg cgtaccgttg atcaggcgaa agcggtggtt gaggaactgg cgcgtcaggg
     4501 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaattc cgtccaacgc
     4561 cttgctggca gagcagttcc tggaatattt cgacggcttc tcaattggct caaacgacat
     4621 gacgcagctg gcgctcggtc tggatcgtga ctccggcgtg gtgtctgaac tgttcgatga
     4681 gcgcaacgat gcggtgaaag cactgctgtc gatggcgatt cgtgccgcga agaaacaggg
     4741 caaatatgtc gggatttgcg gtcagggtcc atccgaccac gaagactttg ctgcatggtt
     4801 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
     4861 aagcctggct gaactgaaga aataaaataa atacccggcg gcgttttgtc gccgggttta
     4921 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgag ttaatcgccg
     4981 cataatatct ttccttaaca aaaacttttg tattttacct gagacagtgc gcggtagttt
     5041 ttctattacc acgatatgtt caggatattt atatttcgcg acccgtttac ggctaaaaaa
     5101 agccaccacc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
     5161 tgaacgttca cctaaacgtt catcgggcat cgcaaccaca caggcatcgt gaattttagg
     5221 atgctgcaat aaaatatctt ctacttcacg gctgctaata ttttcgccgc cgcggacaat
     5281 aatatctttc ttgcgcccgg ttatttttat atagccagcc tcatccatgc ggcagagatc
     5341 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
     5401 ataccccata aacacattgg ggccacgcga ggcttcttca ccttcgcaac ctggcggtaa
     5461 agtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccgtcggt
     5521 gtgcataaag cgcggcaaag gatcgtcgag attcaccacc gcatgcggcg aactttctgt
     5581 ggaaccataa acacttaata atttaatgcc gcgctgctgg cattctcgcg ccactttttt
     5641 ggggattgtg gtacctccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
     5701 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg ccgagcatac aggtgcaacg
     5761 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
     5821 agcaccaatc aaaaaaggtg ctgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
     5881 cgcaggcatc ataaagacat cctgccaggt cagatttagc cgcgcgcaat aagcccgctc
     5941 actggcgaga atattgttat gcgttagcat cacacccttt ggcagaccct cggttccgga
     6001 ggtaaatagc acagcggcta attcatcgcc gtgggtcgtt atcgccgtgg tcagaggggt
     6061 tttgtcggcg agaatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
     6121 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctactgg
     6181 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
     6241 ccacaccagt tctgcttccc gccaggaagg taacagcggc acggaaaccg caccggtttt
     6301 caggcaggca agatagataa cggtaaattc acaccagcct ggcagttgaa atgcgatgcg
     6361 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
     6421 gagcgcgcta taggtgtacg ttgcaccatg attatcgacc acggcaattt tgtctggcat
     6481 cgcacgagcg gtctgctgcc agtaatcggc cagcgaagca tcgccccata acccttgctg
     6541 acgatacgcc gcacgacgtt gttcgttgaa cgttaatgtc actttcattt tggactctcg
     6601 aaacagaaca tcaggcccga gatgcgggcc tgtgggatgc atgattagcc gtagcgaaat
     6661 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt gccgcacagc
     6721 acccgacagg tgccgcactc cagacaaccg gcggaatcga aatgaatgtt tcctgcgtca
     6781 tcctgcttat aaagtccggc agggcaagct ttcattaatt tacggaattc attgatatcg
     6841 ggatttgccg ccaaaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
     6901 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
     6961 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
     7021 ccggctggtt tggtttgccg tcaatggtga acatatcgtt catgatgtcg gcgaccattc
     7081 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cggaaatgct
     7141 gcatatcgcg cataacacaa ctttgttcca gctcacgttt gtattgcgcc agactgctgg
     7201 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagctgatg
     7261 caatggctaa atccatgccg cgaactgtaa aacccaaatt caggcagaag cctgcggcgt
     7321 caccaacgat catcacgcca tcgttaacca gctgcggcac cattgccaga ccgccctccg
     7381 gcaccatatg cgcggaatat tcaagcagtt tgccgctgct aatcagcggg cgaatggcgg
     7441 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
     7501 tcaatccaca aaccagcccc aacgatacgg aatctttgtt ggtatagagg aatccgccgc
     7561 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
     7621 cattaaagcg atcgttgatc tgttctggtg tcagacctat aacctcttta acaccaacag
     7681 cgtaatgatg cggatcggaa gcggaaacca ttcccagcga gcggccaagc atcgagttaa
     7741 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccggcc tggacgccag
     7801 tgaccttgtt tccttcacga accagcgcat cgacgcgtac gcccgggata aactgcgcgc
     7861 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
     7921 acgcgtgttg cggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
     7981 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatgctgcaa
     8041 agcctggaat aattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
     8101 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgc gccatgacca
     8161 gtgcggcaac gctaccggca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
     8221 acatgcgtta tgctcccttc ccgctgcgtt tacagcggga gatgtggttg aaagggaatt
     8281 agcgattcaa ctggctaatc agggcaggga cgactttgta gatatcgccc accagaccgt
     8341 agtcggcata gttgaagatt ggcgcatttt tatctttatt aatggcgaca atcacttttg
     8401 cgccgttgcc gccaaccata tgctggatct gcccggagat ccccagcgtc aggtagagat
     8461 cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc cagttctcgc
     8521 cttcggcaat tggacgtgaa cagccgactt cagcattcag cactgccgcc agttcgtgga
     8581 ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca cgttttgctt
     8641 tgctcaggtc cacgctgctt gccgctttcg cacggcgttc gcgacagaga atttcatgac
     8701 gcggggcaac ataaggtacc gtttctgtcg ggcactgatg agagatatca cgggtgcacg
     8761 gctcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc ttttcctgag
     8821 cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg acaatatcca
     8881 ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt gcggccagag
     8941 ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct ttcagcaggg
     9001 cagcaatgct ttcggcgtaa ttttcagtac gttgcagcgc gtcgttttgc tcaagaacat
     9061 aaatgcattt tggcccataa ggcataaccg cctgcgcctg ctcggtattt tgtacaatgg
     9121 catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa cgttcaggat
     9181 tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg taggcttcag
     9241 ttcagggctt ttttcagatg ttccgccagc tcggcaatgg cctccggcga atcgttatcg
     9301 agaatgatgt gcttacgttt tgtttgcggc ggtacgcgaa taccggtcag ttcagcaagt
     9361 ggtgcgctct ggctccagcc aatatcactt gcctgccact gatttaccgg ttttttaccc
     9421 gcgccgagga tggctttcat cgaaggaata cgtggtacgt taatatcgga ggtgacgcag
     9481 agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt gcgttcaatc
     9541 accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg aagttgcaga
     9601 agttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc ttcaccaaag
     9661 atcagtaaat cgaagccgat cttttcaact gctgtcgcca gcgcctttgc ggtatcgaga
     9721 ggcagtgcat gctcaagttg tgcatcctgc accatataca ggctgtgcgg cccgcgggat
     9781 agcacgtctt tgcgcacttt cgagttctgc aacaaagagc cgccgacggt cagcgcggct
     9841 atctcatcgt catctgttgc aagctggctt gcagcttcaa tggcattgag atcgaactgg
     9901 ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac aacaatgtcc
     9961 tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc tgtgagtcgc
    10021 atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc gatagcggtg
    10081 cggggttttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa aaaatgcgat
    10141 cgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgtg caatcttgca
    10201 ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc gcaatacacg
    10261 ccgtatttta tccggcatat attgtttttg tttgctcatg gaatagggag gttgtcatgt
    10321 atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa acgccacggc
    10381 tttcacgttt cgcatttagc gatgatccaa aatgggagtc tggacatcac gttcatgaca
    10441 atgaaaccga gctgatttac gtcaagaaag gggtagcaag gtttaccatc gattcttcgt
    10501 tatatgtcgc gcatgccgat gacattgtgg tgatagaacg tggcaggttg catgcggtgg
    10561 cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc tttcagtttc
    10621 agggggctga ggaaaatcag ctactgcaac cgcattcttg cccggtaatt gccgcaggac
    10681 agggaaaaga agtcattaaa accttattta atgagctaag tgtgattttg ccgcaaagta
    10741 aaaatagcca aacatcttcg ttatgggacg catttgccta tacgttagca attctttact
    10801 acgaaaactt taaaaatgct tatcgttcgg agcagggata tattaaaaaa gatgttctga
    10861 taaaagatat tcttttctat ctgaataata actatcgcga aaaaatcact ttagagcagt
    10921 tatcgaaaaa atttcgtgcc agcgtcagtt atatctgcca tgaatttacc aaagagtatc
    10981 gtatttcccc tattaactat gttattcaac ggcgtatgac ggaagcgaaa tggtcactca
    11041 ctaatactga attatctcag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc
    11101 actttgccaa actgtttttg cgccatgtcg gctgttcgcc cagcgattac cgcaggcaat
    11161 ttaaaaactg ttttgcggaa caagaaatcc tatctgaatt tcctcaaccg gtaagtcttg
    11221 ccggataaat ctggcggcgc agtttgttaa gaggctgcgc cgcctgcaga ttatttgttc
    11281 tgataatctt tcaggatctg ccgacctgct acgtaaatca taatttcgtc tgtaccgccg
    11341 ccgatacgtt cacaacggac atcacgccag aagcgggaga cgcgcgcctc atcggtatag
    11401 cccagaccgc ccatgatttg aatcgcatca tcaatgactt ccattgcggt acgtgcgcag
    11461 tacagttttg ccagcgccgc gctggtgcgc agtgactgat gctgatcggc ttgccatgcc
    11521 actttcaaca ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc
    11581 tggatcatct ggttatgacc aatgggctta ccaaaagcga tacgctggtt ggcatagcgg
    11641 gcggcatctt cgaatgcgca ttcggcaaag ccggtgctgc gtgcggcgtt gatcaggcgc
    11701 tccatctcaa agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg
    11761 ctctcttcaa cttccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg
    11821 attttatgca gtggattgat tttaatgccg ggtttactgg agtcgaccca ccacagagta
    11881 aaggcctttt tcggatcttt cggttgcgga tcgcgcgcca acaccagcat atatgggtac
    11941 tctttcgcac cggtaataaa ggttttctgt ccgttgatgt aaaccttgcc gtttttacgc
    12001 gtgtaagtgg tagtggcact gttgttgtct gagcctgcgc ctggctccgt caatgccagg
    12061 gcataggcgg gatcacctgt ttccagggtg ctctctgccg ttttacgtag ctgctctgca
    12121 gaaccgaaac ggcgcatact gtgaatacat tgaccgttgg taatcaaaaa tgccggagca
    12181 ccgcattttg acacttccat cagcgccagc atttgggtga cgtaatccgc agggatacca
    12241 ccaaattctt ccggcacgcc aagcatggaa ataccgttat ccgccagtgc ccgcataaac
    12301 tcacgcgggt atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc
    12361 gtaatcagtt cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatcc
    12421 atcattatac tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat
    12481 aaataatctt tcgtccatca gtttcagttc tggcgaaatc actggggtga aatccatttt
    12541 gtcgagaata tctttttgta aatcgacgcc aggagcgatt tcgattaaat gcaggccgtc
    12601 ttcttttaac gtgaatacgg cgcgctcagt gatataacga acatccagcc ctcgctcgag
    12661 agcgattttt ccgctgaaag ttatttccgg tagttcccga ataaatttct tcacccgtcc
    12721 ttcctggacg atatttaatt tgccgtcggt aatttctgtt tttaaactgc ccgcagttaa
    12781 tgtgccgcag aacacgattt tcttggaagt ggcactgata tcaataaatc caccagtgcc
    12841 catgatttta ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa
    12901 actcaaataa caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc
    12961 cagaatggcg cgggtattca cgttcgcgcc aaaggcgatc ccctgtgaag taataccgcc
    13021 aatcggacct gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgggcgac
    13081 caggccaatg ccgtcagcaa taccgacgcc gacattcccc actgcgcctt tgcgcatttc
    13141 gaataatgca cgccgtgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtgct
    13201 gtcatcaaga gtgaagtcac cagaaataaa gcggttaacc ggcgcaccgc catacagttg
    13261 ggtttgatcc ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt
    13321 aggatgcagc gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt
    13381 attgtgcacc gcctgagcaa taaccaatgc gtcgagatac atcacctcat cttcaaaagt
    13441 ggcgtagcct tcgctgtcgc aggtggtagc gcgaatgaag gcgatatctg gcgctatcgc
    13501 tttgtaatag agatattctt tgttatcaaa ctcgaccagt ttaatcagtt cttctttagt
    13561 gacttcattc agtttgccgc cttgctggcg tggatcgaca aatgtcccga tgccaatatc
    13621 actaataata ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg
    13681 tggatagtta taagcaataa ttttattttg ttctgcgaga tcagaaatac gcggcgattg
    13741 tccccagtga ccgcataatg cccatttcac cagaccttct tgcgccagcg gactaatacc
    13801 acggtcggcg cgatcgccaa gccctgttgg actaataatc gataaattac gtggtgtttg
    13861 agtctgttta tatttatcag caagagcagt aattaacgtg gtggcttcca gaataccgcc
    13921 gccagcgcct aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc
    13981 cgacaggacc ggcactcgtc cattaatacg aggtggtttt acaggtttca ttgttaattc
    14041 ctgattcagg tgtgattttc cctacgccag atggagaatt agcg
//
LOCUS       DADTFG010000128        13704 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.129, whole genome shotgun sequence.
ACCESSION   DADTFG010000128 DADTFG010000000
VERSION     DADTFG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.129"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(87..1046)
                     /gene="ybtA"
                     /locus_tag="J1138_003556"
     CDS             complement(87..1046)
                     /gene="ybtA"
                     /locus_tag="J1138_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HBA8455312.1"
                     /translation="MMESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            1303..3015
                     /gene="ybtP"
                     /locus_tag="J1138_003557"
     CDS             1303..3015
                     /gene="ybtP"
                     /locus_tag="J1138_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HBA8455313.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLAHKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            3002..4804
                     /gene="ybtQ"
                     /locus_tag="J1138_003558"
     CDS             3002..4804
                     /gene="ybtQ"
                     /locus_tag="J1138_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HBA8455314.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLAPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            4797..6077
                     /gene="ybtX"
                     /locus_tag="J1138_003559"
     CDS             4797..6077
                     /gene="ybtX"
                     /locus_tag="J1138_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HBA8455315.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRVLLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            6105..7409
                     /gene="ybtS"
                     /locus_tag="J1138_003560"
     CDS             6105..7409
                     /gene="ybtS"
                     /locus_tag="J1138_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HBA8455316.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(7603..8843)
                     /locus_tag="J1138_003561"
                     /pseudo
     CDS             complement(7603..8843)
                     /locus_tag="J1138_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(9005..9080)
                     /locus_tag="J1138_003562"
     tRNA            complement(9005..9080)
                     /locus_tag="J1138_003562"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9045..9047),aa:Asn,seq:gtt)
     gene            complement(9181..9978)
                     /gene="mtfA"
                     /locus_tag="J1138_003563"
     CDS             complement(9181..9978)
                     /gene="mtfA"
                     /locus_tag="J1138_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBA8455317.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVAIAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            10072..10161
                     /locus_tag="J1138_003564"
     tRNA            10072..10161
                     /locus_tag="J1138_003564"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10106..10108,aa:Ser,seq:cga)
     gene            10440..11276
                     /locus_tag="J1138_003565"
     CDS             10440..11276
                     /locus_tag="J1138_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455318.1"
                     /translation="MKRSEIRKALEAWFDVERYEAIEKLTLQQFYVEVERRILAYRML
                     LSRNTIPTFNRLMLDDYRNKILSGEIFFSGDTATLGHELARTYAVNPTTRSHAQFYAK
                     TLALTEATPELSALSESEFLSEYLKETSLNNLSRITVDIHLEEASTEEIIEHLKVLIP
                     QWKRQLKMKSPAPREYRFGKSTFRKIIEYRLIPMMDLIFWGEDNGIKIPLSLISSLLH
                     EDSDNDRDEGMLKATDYPLAMAFLTDASYLKSLEDYMMENNQLKDSPVEKHVEDDRNK
                     KK"
     gene            11492..11710
                     /locus_tag="J1138_003566"
     CDS             11492..11710
                     /locus_tag="J1138_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBA8455319.1"
                     /translation="MKENIETSVKRFIRVPDVLNRVGFSRTTLYERIKEGNFPDRVKI
                     GPRCVAFVESEIDEWIEAAIRNSRQNAA"
     gene            11792..12082
                     /locus_tag="J1138_003567"
     CDS             11792..12082
                     /locus_tag="J1138_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455320.1"
                     /translation="MKIELITTKQFIEQAECYFRSYMDGLRRNAPDDFYYFLNNKYNM
                     NDIMESIIKKTRYHFYDDTEEDQSNRIYGEVSHCKVKQHLRQLWIIYKCVYR"
     gene            12135..12431
                     /locus_tag="J1138_003568"
     CDS             12135..12431
                     /locus_tag="J1138_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455321.1"
                     /translation="MNTFKNKNTEIFYVVSLHVYAEIFNSKDKTTSNMIITHVMDHEF
                     VCKLIDLAMRNAEKHLLKKAWKKNAAEKLSEVDFKEVRQALAKMHYTVLAESIC"
     gene            12505..12747
                     /locus_tag="J1138_003569"
     CDS             12505..12747
                     /locus_tag="J1138_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA8455322.1"
                     /translation="MIDEFHVMYMYKKIQAEAATTDIKTLEQLLKKFRSVVNERREEY
                     YQEIGEKKAHKLKMKRLQRMLKKMACDRVSPEDLKE"
     gene            12856..13206
                     /locus_tag="J1138_003570"
     CDS             12856..13206
                     /locus_tag="J1138_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455323.1"
                     /translation="MGFEAKKKHAIAIMESKRMWRSNYAPPLLHLAWKAGLKIPPLPF
                     ASFWQITLLMGGWFGPAWGICMWFFTWKDTGIQLGTAFVSSVVAGICFGVLMAAYHWW
                     RKVVNNLPDWDSLG"
     gene            complement(13270..13674)
                     /locus_tag="J1138_003571"
     CDS             complement(13270..13674)
                     /locus_tag="J1138_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HBA8455324.1"
                     /translation="MSEALKALNNIRTLRAQARETDLATLEEMLEKLTTIVEDRREEE
                     NSARKEQEERQAKLEAFRQKLLEDGIDPTELLASVGSSQPKTKSTRAPRPAKYKYTDE
                     NGNEQTWTGQGRTPKAIAAAIEAGKTLEDFAI"
BASE COUNT         3262 a         3270 c         3746 g         3426 t
ORIGIN      
        1 gggttggtaa caggcggaaa gaacagaggc ccggatgggg tatccgggcc tctgtcaggg
       61 aggagtttag gggggcgcga ccccggttac atcccgcgtt taaaggtcga aggagttacg
      121 ccaaactgtt tctggaaggc ggcagagaaa tggctggcgt tggcatagcc cagatcggcc
      181 gccaccgtca tgacggaggt attgccgttg gccaggcgtc ttctggcttc ctgcattcgt
      241 tcagcctgaa acaggccata gacgctgttg ttgaacagaa cgcggaagcc tcgcttaatt
      301 ttgagaacgc tcagaccgga ctctctggcc agctccgcga cgcccggcgc ttgcgtcaga
      361 tcagccagca gcagatcctt cgcccgcagc agtttctggc gttccatcgg gttgaagtgg
      421 caggagaggc aactggcgtc ttcgcgatgt tcatccagca ccaggctgag catgaccaga
      481 ctttgcccct gcaaccatag cgtcgacggc ttttgttcat gactgaagcc gccgtgcatg
      541 cgcatcagcg cgtgacgcaa ggcctgagcc gttaagtggg tttcagcatt acaacgctgc
      601 tgctggcaac agcagtggga gtcgatctta ttcttaatga ccgcgctaat atccggcacc
      661 cacagcgcga gggtttcagg gctgaaggag agggtaatgc tctcgaatcg gccacaatag
      721 gaggcgctgc cgcgacagtc tggcgtatgg gtaatgtaat ggcgattgcg cgcaagctcc
      781 cagtcggtgt ggcgccggag tccctgaatc gcaaagcgcg atgtgccttc caggatgcac
      841 gagaagttaa tcctgcccag atcgtcgtgt agctgcaacg tctcaggccg cgcaaaatgg
      901 cccctccaga gcaaaatatc cataccttcc tgcaacgaac agcgcagcag ttcacactga
      961 gcaggaatat tgccatcgtt tgcaggtttc ccgaccacca actggtgaat agagatttca
     1021 gattgcgttt gcggtgactc catcatgacc tggttatctc cctgtggctt agaccaatgc
     1081 gggtataaat agaaccgaat ggggtagatg agttattgat aatggttatt attcacatta
     1141 attctcagaa cgccaccctg aattgtcgtt tttggcgtcc gtaaaaattc atcaggaaat
     1201 tcagttactc ccatgtcatc tcaatcatcc aatactgaat cgctgtcccg atttcctctc
     1261 tggcaggtga ttacaccggt tcgcaggaag gttattttag ctatggcgct ggcggggctg
     1321 gcggcattaa ccagcctggg ggcgctcctt tttctggcgt ggagcctgcg tgacattcgc
     1381 gcgacgcctg acgctattcc ggcctggccg ctgggcggcg tgatcggctg cgtggtctta
     1441 acttttgttc tgcgcttaca ggcgttcaac acctctcatt acgcggcttt tcatctggag
     1501 aacattctgc gcagcaggtt agcccataaa gcattgcagc ttccgcctgg cgtgttacag
     1561 caaatgggca gcgggtcagt ggcgaaagtg atgctggatg acgtgaagtc gttacatatt
     1621 tttgtggccg acagcacgcc gctctatgcc cgcgcgatca tcatgccgct ggcgacaatc
     1681 gttatcttgt tctggctgga ctggcggctg gcaatcgcga cgctgggggt actggcgttt
     1741 ggatcggttg ttctcgtgct cgcccgccag cgttcggaag atatggctca gcgttatcat
     1801 aaggcgcgcg agcaggttag cgcggcggtt attgagttcg tgcaggccat gcccgtggtg
     1861 agaacgtttg atagcggcag caccagtttt ttgcgctatc aacgcgccct tgaagagtgg
     1921 gtcgatgtgc tcaaaacctg gtatcgcaaa gccggttttt cagcgcgttt ttccttctcg
     1981 attctgaatc ctctcccgac cctgtttgtc ctgatctggt cgggatacgg cctgttgcac
     2041 tatggcagtt tcgattttat cgcgtgggtg gccgttttac tgctgggcag cggaatggcc
     2101 gaagccgtaa tgccaatgat gatgctcaat aacctggtcg cgcaaacgcg tttaagcatt
     2161 cagcgtattt atcaggttct cgcgatgccg gagttatcgc tgccgcagtc tgaccagcag
     2221 ccgcaagagg cgagcattac ctttgagcag gtgagctttc attatccgca agcgcgtact
     2281 ggcgccgcgt tgcaggaggt gagctttcat gtgcctgccg ggcaaattgt ggcgctggtc
     2341 gggccaagcg gcgccggaaa aagcaccgtg gcgcgcttgc tgctgcgtta cgccgacccg
     2401 gacaaaggcc atatccgtat tggcggcgtg gatctgcgtg atatgcagac ggacaccctg
     2461 atgaagcaac tctcgtttgt gtttcaggac aacttccttt ttgccgacac gatagccaat
     2521 aacattcgtc tgggcgcgcc ggatacgccg ctggaggcgg ttatagcggc ggccagagtg
     2581 gcgcaggccc atgattttat tagcgctctg ccagaaggtt acaacactcg agtcggggaa
     2641 cgtggggtat ttctctccgg cggccagcgg cagcgcatta ctatcgcccg ggcgcttttg
     2701 caggatcgcc ccatcctggt gctcgatgag gcgacggcgt ttgctgaccc ggaaaacgaa
     2761 gcggcgctta tcaaggcgct cgcggcggcc atgcgtggcc ggacggtcat catggtcgcg
     2821 catcgtctct caatggtgac tcaggccgat gtgatattgc tgttttccga cggacagctc
     2881 agggaaatgg ggaaccatac gcaactgttg gcgcagggcg gcctgtatca acggctctgg
     2941 caacactatc agcaggcgca gcattgggtg ccgggtggaa cacaggaaga ggtggtggaa
     3001 aatgaaagac aataatcctg cggataacct ggcctggcgc gtcatctggc gccagcttat
     3061 ctccagcgtt ggcagtcagg ccaggatgct gcgacgcagt atgctggcgc tgttgctggc
     3121 ggcattcatg caggggatcg cctttgcctg tctttatccg atcattgatg cgctgttacg
     3181 gggagacgcg ccgcaacttc ttaactgggc tatggccttc agcgtcgccg caattgtgac
     3241 gctggtgcta cgctggtatg gcctgggctt tgaataccgt ggtcatctgg cgcaggccac
     3301 ccatgagttg cgcctgcgac ttggcgagca gttacgccgc gtgccgctgg agaagctcca
     3361 gcgcggcagg gcgggtgaaa tgaacgcctt gctgctgggc agcgtggatg aaaacctcaa
     3421 ttatgttatt gcgatagcca atattttgct gctcaccatt gtcacgccgc tgacggcgtc
     3481 gctggcgaca ttgtggatag actggcggct ggggctggtg atgttgctga tcttccctct
     3541 gctggtgccg ttttattact ggcgccgccc ggcgatgcgg cgacaaatgc agacgctggg
     3601 ggaagcgcac cagcgtctga gcggcgatat cgttgaattt gctcagggga tgatggtatt
     3661 gcgcacctgc ggcagcgatg ccgataaaag ccgggcgctg ctggcgcatt tcaatgcgct
     3721 ggaaaactta cagacccgca ctcaccgtca gggcgctggc gcgacgatgc tgatcgccag
     3781 cgtcgtggag ttgggcctac aggtggtggt gttatccggg atcgtctggg tagtgacggg
     3841 cactctgaac ctcgcctttt tgattgccgc cgtcgcgatg attatgcgct tcgcagaacc
     3901 gatggcgatg tttatcagct acacctcggt tgtggaactg atcgccagcg ccctgcaacg
     3961 gattgagcag tttatggcga tagcaccgct tcccgtcgca gagcaaagcg agatgccgga
     4021 acgttacgat atccgctttg acaacgtcag ctatcgctac gaagaaggtg acggccacgc
     4081 gcttaatcat gtttctttga cgttcccggc agccagtatg agcgcgctgg tgggtgcctc
     4141 cggcgcaggc aaaactacgg tcaccaaact gttaatgcgc tatgccgatc cgcagcaagg
     4201 gcagatttct attggcggcg tcgatattcg ccgcctggcg ccggaacagc tcaatagcct
     4261 gatttctgtt gttttccagg atgtctggct gtttgatgac acgctgctgg cgaatatccg
     4321 tatcgcgcgc ccacaggcga cgcggcagga ggtagaagag gccgcccgcg cggcgcagtg
     4381 tcttgagttt atttcccgtc ttccgcaagg ctggctgacg ccaatgggag agatgggcgg
     4441 ccagctatcg ggcggcgagc gccagcggat ttccattgcc agagcgttat tgaaaaacgc
     4501 gccggtcgtc attctcgatg aaccgactgc cgcgctggat attgaaagcg agctggcggt
     4561 gcaaaaagcg atcgataacc tggtttacaa ccggacggtg attatcatcg ctcaccgttt
     4621 atccaccatc gccggggccg gaaacattct ggtgatggaa gagggacagg tggttgagca
     4681 gggtactcat gcgcaattgc tctcacatca tgggcgttat caggcgctgt ggcaggcgca
     4741 aatggccgcg cgcgtgtggc gcgacgacgg ggtttccgcg tctggagagt gggtgcatga
     4801 gtgatgttca gtcgaatgtg aaaccgctga cgttgacgac cgggcgggtg atttttgcta
     4861 ttgccggcgt ctatgtgacg cagagtctgg tatcggcgct gtctatgcag tccttacccg
     4921 cgctggtgcg cgctgctggc ggttcgctgg cgcttgccgg tgcgacaacc ctgtttatgc
     4981 tgccctgggc gctgaagttt atttgggcgc cgtggatcga gcgctggcgg cttccgcccg
     5041 gtagccagga acgccgttcc cgcatgttaa tcctgcgtgg tcaggtcgcg ctagcggcga
     5101 tcctgactat tgccgcagcg attggctggt ttgggcgaga agggggattc cccgatacgc
     5161 aaatcgtcgc gttatttgtt ctgtttatgg tggcaggcac ggtcgcctcc accattgata
     5221 tcgccagcga cggcttttgt gtcgatcaac tgacccgcac gggttacggc tggggaaaca
     5281 gcgtgcaggt cggcggcagc tatctgggaa tgatgtgcgg cggcggggtg ttcctgatgc
     5341 tgtcggcggc atccggctgg cctgtcgcca tgctgatgat ggcggtgctg attatggcgc
     5401 tgtcactccc gctgtggcgc attacggagc cgacgcgaac agcgactatc ccgcatgttc
     5461 cggcgttagg ttatgcgcta aggaggaagc aggcgcgcct gggcttactg ctggtattga
     5521 tgctgaattc aggcatgcgg tttgtgctgc ctcttctggc accgctgttg ttggatcatg
     5581 ggttgagcat gtccgcattg ggcgcgctgt tcagcggcgg caatattgca gcgggcatag
     5641 caggaacgct ggctggcgga ttgctgatga aatacacctc acccggcaga gtgctgttga
     5701 cggcttatgg cgtccagggg atcgcgctgc tggcggtggt gatgacgctc atgatggcgc
     5761 cgggtcatct gctgctgcag attctccagt gtctggtcat tgtccagtcc atttcgctgg
     5821 cctgcgcgct ggtctgtctt tacgccacgc tgatgtcgct ttcatcgcct ttgcaggccg
     5881 gtgtcgactt caccctcttt caatgtactg atgcggcaat cgccattctg gcaggcgtta
     5941 tcggtggcgt tgttgctcaa cattttggct atgcggcctg cttcctgttt gccggggcat
     6001 tcacgttgct ggcggcgtgg gttgcttata tccggctgca ttcggcaaga gaactgatga
     6061 caagcgcaat tgattgatcc gcgaataaca cagagaggta agggatgaaa atcagtgaat
     6121 ttctacatct ggcgttacca gaggaacaat ggctaccgac gatttctggc gttttacgcc
     6181 agttcgcaga agaagagtgt tatgtctatg agcgtcaacc ctgttggtat ttaggcaaag
     6241 ggtgccaggc acggctgcac attaatgccg acggaacgca ggcgacattt attgatgatg
     6301 ccggggagca aaaatgggcg gtggattcca ttgccgactg cgcgcgtcgt tttatggcgc
     6361 atcctcaggt gaaaggacgt cgggtatatg gacaggttgg gttcaacttt gcggcgcatg
     6421 cgcgggggat tgcctttaac gccggggagt ggccgctgct gacgttaacc gttccccgtg
     6481 aagaacttat ttttgaaaag ggaaatgtca ccgtttatgc ggactccgcc gacgggtgcc
     6541 gacgtttgtg cgagtgggta aaagaggccg gtacaacgac gcagaacgca ccactggcgg
     6601 tggataccgc cctcaatggt gaggcatata aacaacaggt tgcgcgcgcc gttgcggaga
     6661 tccgccgtgg cgagtatgtc aaagtgattg tctcgcgcgc cattcccctg ccatcgcgga
     6721 ttgatatgcc cgccacgctg ttatacgggc ggcaggcaaa cacgcctgtg cgctcgttta
     6781 tgttccgtca ggaaggacgc gaagcgctgg gctttagccc ggaactggtg atgtcagtga
     6841 cgggcaataa agtggtcact gaaccgcttg cgggcacccg cgatcgcatg ggaaacccgg
     6901 agcataataa ggcgaaagag gcagaactgc tgcacgacag taaagaggtg cttgagcata
     6961 tcctttctgt caaagaagct attgctgaac tggaggccgt ttgccagccg ggcagcgtgg
     7021 tggttgaaga tttaatgtcg gttcgccagc gcggcagcgt tcagcatctg gggtctggcg
     7081 tgagcggtca gcttgcggaa aacaaggatg cctgggatgc gtttaccgtg ctgtttccgt
     7141 cgattaccgc ctcaggtatc cctaaaaatg ctgctctgaa cgcgattatg caaattgaga
     7201 agacgccgcg agagctttat tccggcgcaa tcctgctgct ggacgatacg cgctttgatg
     7261 cggcgctagt tctgcgttcc gtatttcagg atagccagcg ctgctggata caggcggggg
     7321 cgggaatcat cgcgcaatct acgccggaac gcgaactgac agaaacccgg gagaaattag
     7381 cgagcattgc gccctattta atggtgtagg agtaactgtt ttctaacctc acaaccagac
     7441 tgtccaaaga caattgagtc gctctttgat tcctctgtga ctctattgtc ttcttttggg
     7501 ataaaatagg tgtttttcgt catgaagttg ataaaagtct atatagaacc acctcttgtg
     7561 gaggtggtgg taatttgcca gagtgtggga ttcgttagta tactatggga taaacttatt
     7621 tttaccaatt gtataagggg gtgcataaga ttctcggcat gcttccagat aatccgacca
     7681 ccactgcatc atcgctttgc gggcttccag gtgctctgcc ttatgaatat aagccatgcg
     7741 cacggtattg cgctcctgat gactcatctg gcgttctact gcgtcctttg cccataaacc
     7801 cgattccatc agcgcactgc atgccattgc ccggaagccg tgaccgcaga tatccttttt
     7861 cgtgtcgtaa cccatcaccc gcagtgcctt attgaccgtg ttttcacaca ttggcttata
     7921 cgggttatgg ttgccgggga agatcagttc attattaccg gtgatatcct taatctgttt
     7981 cagaatggcg atggactgtt ctgagagggg gacgatatgc ggcattcgca tttttgcccc
     8041 gcggccggaa taacgcacgc caataatggg ttctcgcgtc gcgggtatcg tccagactcg
     8101 gtttgtgaaa tcaatctctg accagcgggc gaaacgcagt tcactggagc gaatgaacac
     8161 atgcagcatc agcagaacgg catgccgggt cagttcacgg ccctgatgat atgccccaat
     8221 acgttcaagc agttcaggca gccgctccag cggcagggca ggatagtgcc gtctgacagg
     8281 aggtgtggtc acgccgccaa ggtttgctgc agggttcgta tcgattaact cctgatggac
     8341 cgcatggcgc attatgttac tcaggtgctg ccgtgtgcgg gacgcaacct ccagcagacc
     8401 tttttcctcg atccctttca gcaggtcaat gaaatgacgg ggtttaagtt ctgatacagg
     8461 taggttcccg atgaccggaa agatgtgatt gttcaggctg gcaagcagac ggtcggcggt
     8521 gttctgcgac catttcctgt tacttttatg ccacgccagc gccacgtttt taaaaacttt
     8581 ctccggtgtt cgtgagccac gttcagcagc ccgctgctgt accgggttga tattcagcgc
     8641 cagtatttta cggatacctt cacgttgctg tcgcgcatca gacagggaga tggctggata
     8701 ggcacccagc gcaatgcggg attctttacc gctaatacgg tatttgagat accagtggcg
     8761 ggagccaccc ggcttgaccc gcagatacag accgtgagaa cagaaggctt gagggtgcgg
     8821 atttttgcgt cggtaaggga tatatggggg tcactccatt atcgaactaa cctgacccca
     8881 gatttgacca ccaatttttc ccgatgcgta gggtaaaatc aaaatgcacc gagaagagtt
     8941 ttcacactaa cctgttgaat caacatcaga taaagattcg caaggatgca tgaaaacaga
     9001 aaattggctc ctctgactgg actcgaacca gtgacatacg gattaacagt ccgccgttct
     9061 accgactgaa ctacagagga atcgtgtgaa cggggcgaat attacttagc ggtaccttgt
     9121 ctgtcaacac taaatttcat aagccatttc aattggttaa ttaaccttca aacttgttaa
     9181 ttaatgaaca ttcgtcgccg aaaacgagtc tgtatcatta gcgtgatgca gtctctgcaa
     9241 aggatcttgt tgataaaatt gacagaaacg ttgccacaat gaagggaaac gaggagcaaa
     9301 aagttctggg gcgctaaaga aatattcaga aagtacggca aaacattcag caggatcact
     9361 ggcagcataa gcatcaatgc tcgccgcatt ctcaccaact aattcgattt cttcctgaat
     9421 gttgttcatt gcagcatgaa gatcatgttc ccagccagca acctcacgca acgaaataaa
     9481 gggaactccg ctggcgcgat cgccgttacg ggtgtccagc ttatgagcga cttcatgaat
     9541 aatcaggtta aaaccagaag catcaaaaga atcttgtata tccaaccagt tcaaaacgat
     9601 aggcccttgc tgccagctct gacctgactg aacaatacgt tggttatgca ccagaccgat
     9661 atcgtcttcc cattcatcat cgaccacaaa tggcgcagga taaattaaga cttcatgaaa
     9721 accatccagc cattccagtc ctaactccag aacgggcagg caaaatagaa gtgctatccg
     9781 gcagcttctt aatgaattca gctcaaagcc ctgtaaagga acaagccgtt tttgctgtaa
     9841 aaaacgttcg gcaatagcga ccaatttgct ttgttcctgt tctgtcagac acgttaaaag
     9901 ggggatcgat agtgcttcct gccagggaag ggcagtttga tgtgctgatt cttgtacttt
     9961 ccagggccac ttaatcatcg ttttgctcgc aaactcgtca cttgaacaaa attgcacgga
    10021 cagggactgt taaaatgcca aatttcctgg catcatggca accatctgaa cggagagatg
    10081 ccggagcggc tgaacggacc ggtctcgaaa accggagtag gggcaactct accgggggtt
    10141 caaatccccc tctctccgcc aatcattcaa caaaatcaat aacttacaaa gcatttttga
    10201 ttttgtcatg cataaatccc tgcattaaaa ttcctttctc tcgctcaatt tttcttacta
    10261 ctgatgctgt tttttaccat tttgctgcgc gtcgggcatc cacttttatg ctctcgagtt
    10321 cacactctta acaacaactt agccattccc ccgtctctga tgacaaaaca gttgaacttc
    10381 aattgttcca aacggatcct gtgaagatat atcaaaggaa atggattgct ggaggatgga
    10441 tgaaaagaag tgaaatcagg aaagcactgg aagcatggtt tgatgtggaa cgatatgagg
    10501 cgatagagaa actcacctta cagcagtttt atgttgaggt tgagcggcgt atcttggctt
    10561 acagaatgtt gttgagccgg aacaccatac ctacctttaa ccgattaatg ctggatgatt
    10621 accgcaacaa gatcctcagc ggtgaaatct ttttcagtgg tgataccgct acccttggac
    10681 atgagcttgc cagaacctac gcggtaaatc ctacgacacg aagccacgcg caattttatg
    10741 cgaaaacgct ggcattaacc gaagccacgc ctgagctttc cgcgttaagc gaatccgaat
    10801 ttctgtccga gtatctaaaa gaaacatccc ttaataatct ttcacgcatt actgtcgata
    10861 ttcatctgga agaggcatca actgaagaaa tcattgaaca tctgaaagtg ctgatccctc
    10921 aatggaagag acagcttaag atgaagtctc cagcgccaag agagtatcgt ttcggcaaaa
    10981 gcacattcag aaaaatcatt gaatatcgtc ttatcccgat gatggacctg atcttctggg
    11041 gagaagataa cggcattaaa atcccgcttt cactgatttc ttcgctgctt cacgaggaca
    11101 gcgacaacga tcgtgatgaa ggaatgctaa aggcaacgga ctacccgtta gcaatggctt
    11161 ttctgacgga tgcgagctat ctgaaatcgc ttgaagatta catgatggaa aacaaccaat
    11221 tgaaagactc ccctgttgaa aagcatgtag aagacgacag gaataagaaa aaataagcca
    11281 agtaaaaggg ttacgttcgc tccgagcgaa cgtgatctca tccagtgtta accgttatct
    11341 ttccatccgt gccccttcga atttttcaaa aaaaacttct ttgaacgtcc ctaaattacc
    11401 ataagcaaaa tcgctatcat tgttcacgtc gaaacaacat agagaaaagc gaaatctcat
    11461 agagattact tacaataata gaggacagac aatgaaagaa aatattgaaa ccagcgtaaa
    11521 acgatttatc cgcgttcctg atgttttaaa tcgagtggga ttcagtcgca cgaccttata
    11581 tgaacgtatt aaggaaggaa actttccaga cagagtaaaa ataggtccga ggtgtgtcgc
    11641 ttttgttgaa agtgagattg atgaatggat tgaagcggct attcgaaact cacgccagaa
    11701 cgcagcataa aaacagaaac aagatctcgt gtcgaacaga taactgttct cacatggcat
    11761 cctattatcc taagcaataa tggagaaaaa tatgaagata gagcttatta cgacaaaaca
    11821 gtttattgaa caagccgaat gttatttcag gagctatatg gacggattgc ggaggaatgc
    11881 accagatgat ttttattatt ttctcaacaa taaatacaat atgaatgaca ttatggaaag
    11941 tatcattaaa aaaacgagat atcattttta cgatgacaca gaagaagacc aaagtaatcg
    12001 gatttatgga gaagtaagcc actgtaaggt aaaacaacac cttcgtcagc tttggattat
    12061 ctataagtgt gtataccgct aatatttata caaatatttt gctattccaa tattaaacta
    12121 aagaggaaat aaaaatgaat acatttaaaa ataaaaatac tgaaatcttt tatgttgtgt
    12181 ccttgcatgt ctacgctgag atttttaact caaaggataa aacgaccagc aatatgatta
    12241 taacgcatgt aatggatcat gagtttgttt gtaagctaat cgatttggcg atgagaaatg
    12301 ctgagaagca tcttctgaaa aaagcatgga aaaagaacgc ggctgaaaaa ctatctgagg
    12361 tggattttaa agaggttagg caggcattag ccaaaatgca ctacactgtt ctggcggaat
    12421 caatatgctg atatattctg atgtctatcg gccccttctt ggggctgata acaaatctta
    12481 tttgtctgtc caaggagcaa aaaaatgatt gatgaatttc atgtgatgta tatgtataaa
    12541 aaaatccaag cggaagccgc aactactgac atcaaaacac ttgaacagct tttgaaaaag
    12601 ttcaggagcg tggtcaacga acgtcgtgaa gaatactatc aggagattgg tgagaaaaaa
    12661 gctcataagt tgaaaatgaa aagattacaa agaatgctta agaaaatggc atgtgaccgg
    12721 gtttcgccag aagatctaaa ggaatgacta tttgagttat ttttgactta catacatgga
    12781 attgataatc attattgtct attgtgcttc tttatcaata gatagatgag taaaggtgga
    12841 tctggaggga accgtatggg ctttgaagct aaaaagaaac acgctattgc catcatggaa
    12901 agcaagagga tgtggcgaag taattatgcc cctcctttgc tacacctcgc atggaaagct
    12961 ggactcaaga taccaccgct accttttgcc tcattctggc aaatcacttt gttgatgggt
    13021 ggatggtttg gtccggcatg ggggatctgt atgtggtttt ttacttggaa ggatacaggg
    13081 atacaactgg gcactgcatt tgtctcaagc gtcgtcgcag gtatttgttt tggtgttctt
    13141 atggctgcgt atcattggtg gcgaaaggtc gttaacaacc tgccagactg ggatagtctt
    13201 ggatgaataa tcggatattt tccgggcctg ttatcttgag aacagtgaag cccggatatg
    13261 ctgtagcggt tagatagcaa agtcttccag tgtcttacca gcttcgatag cagcggcgat
    13321 tgctttagga gtacggccct gacccgtcca agtctgctca ttaccgtttt catctgtata
    13381 tttgtattta gcaggacgag gagcacgagt tgatttggtt ttaggctggg atgaaccaac
    13441 tgaagcgagt agttctgtag gatcgatacc gtcttctaac aatttctggc ggaaggcttc
    13501 cagtttagcc tgacgttctt cttgttcttt acgggctgaa ttttcttcct cacggcgatc
    13561 ttcaacgatt gtggtgagtt tttccagcat ctcttccaga gttgccagat ctgtttcacg
    13621 agcctgtgcg cgaagtgtac gaatattgtt cagtgcctta agagcttcac tcatagtatg
    13681 tcatccggtg ttttaaaata ggcg
//
LOCUS       DADTFG010000129        13689 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.130, whole genome shotgun sequence.
ACCESSION   DADTFG010000129 DADTFG010000000
VERSION     DADTFG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.130"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(1..254)
                     /locus_tag="J1138_003572"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="J1138_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            777..2705
                     /gene="thrS"
                     /locus_tag="J1138_003573"
     CDS             777..2705
                     /gene="thrS"
                     /locus_tag="J1138_003573"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBA8455325.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2709..3251
                     /gene="infC"
                     /locus_tag="J1138_003574"
     CDS             2709..3251
                     /gene="infC"
                     /locus_tag="J1138_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBA8455326.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3348..3545
                     /gene="rpmI"
                     /locus_tag="J1138_003575"
     CDS             3348..3545
                     /gene="rpmI"
                     /locus_tag="J1138_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBA8455327.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3598..3954
                     /gene="rplT"
                     /locus_tag="J1138_003576"
     CDS             3598..3954
                     /gene="rplT"
                     /locus_tag="J1138_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBA8455328.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            4077..4121
                     /locus_tag="J1138_003577"
     CDS             4077..4121
                     /locus_tag="J1138_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HBA8455329.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4405..5388
                     /gene="pheS"
                     /locus_tag="J1138_003578"
     CDS             4405..5388
                     /gene="pheS"
                     /locus_tag="J1138_003578"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBA8455330.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHITLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5403..7790
                     /gene="pheT"
                     /locus_tag="J1138_003579"
     CDS             5403..7790
                     /gene="pheT"
                     /locus_tag="J1138_003579"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBA8455331.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVSNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLDADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARIYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7795..8094
                     /gene="ihfA"
                     /locus_tag="J1138_003580"
     CDS             7795..8094
                     /gene="ihfA"
                     /locus_tag="J1138_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBA8455332.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8195..9175
                     /gene="btuC"
                     /locus_tag="J1138_003581"
     CDS             8195..9175
                     /gene="btuC"
                     /locus_tag="J1138_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBA8455333.1"
                     /translation="MLTLARQQQRQNFRWLLCLSVLMLLALLLSLCAGELWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9237..9788
                     /gene="btuE"
                     /locus_tag="J1138_003582"
     CDS             9237..9788
                     /gene="btuE"
                     /locus_tag="J1138_003582"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBA8455334.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9788..10537
                     /gene="btuD"
                     /locus_tag="J1138_003583"
     CDS             9788..10537
                     /gene="btuD"
                     /locus_tag="J1138_003583"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBA8455335.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10615..11079
                     /locus_tag="J1138_003584"
     CDS             10615..11079
                     /locus_tag="J1138_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HBA8455336.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11326..12039
                     /gene="ydiV"
                     /locus_tag="J1138_003585"
     CDS             11326..12039
                     /gene="ydiV"
                     /locus_tag="J1138_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBA8455337.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELSKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12102..13538
                     /locus_tag="J1138_003586"
     CDS             12102..13538
                     /locus_tag="J1138_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBA8455338.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVESGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLDDEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13542..>13689)
                     /gene="hemP"
                     /locus_tag="J1138_003587"
     CDS             complement(13542..>13689)
                     /gene="hemP"
                     /locus_tag="J1138_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBA8455339.1"
                     /translation="SHKTASPQPIRRITSQTLLGPDGKLIIDHDGQEYLLRKTQAGKL
                     LLTK"
BASE COUNT         3413 a         3188 c         3677 g         3411 t
ORIGIN      
        1 gtgaagtggt aaagctaatg ataaaaaggg cagtggtata acaattgtgt gatatttttt
       61 cgacagcttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taaatcagag
      121 gtatcaagta gggtgatgag ctggcgcgca aattgattaa taggctctga tgaaggcgtt
      181 agtatgtcgc tatcgatgtg cattctaaga ggagactcat aatgagttcc gataatgata
      241 tcgtttttac tcataatcta ttcgccttat atatgtctgg agattttgag gatgatgatt
      301 gattattatc gccagaacga aatgcgcacg aaaaattgct ataagaatta tgtcactttt
      361 atcattgcga tatgactact tcgtcattaa tctcatgatc gtttaaatac taccgccaaa
      421 tcacgcaacg aactttgctg gattcagttt atgctgctgt aaatccgctc gtagtaaacc
      481 tttcagacac tctgtgatgt tatcagtggt tctttaagcg ttttgctggt gtactcacta
      541 caaacgaatt gcgaatcgaa tcaatgtgaa acggaaaggt acaatctccc tttttttagt
      601 gttaacatcg ttcaaccggg tggaaggtga gaggttaatg tctttcattt gtgtacctga
      661 ttgcgaacca atttagcatt tgttggctag atggtttcgc aatgaactgt taataaacaa
      721 atttttcttt gtatgtgatc tttcgtgtgg gtcaccactg caaataagga tataaaatgc
      781 ctgttataac tcttcctgat ggcagccaac gccattacga ccacgctgta agccccatgg
      841 atgttgcgct ggacattggt ccaggtctgg cgaaagcctg tattgcaggg cgcgttaatg
      901 gtgaactggt tgatgcttgc gatctgattg aaaacgacgc acaactgtcg atcattaccg
      961 ccaaagacga agaaggtctg gagatcattc gtcactcctg tgcgcacctg ttagggcacg
     1021 cgattaaaca actttggccg cataccaaaa tggcaatcgg cccggttatt gacaacggtt
     1081 tttattacga cgttgatctt gaccgcacgt taacccagga agatgtcgaa gcactcgaga
     1141 agcggatgca tgagcttgct gagaaaaact acgacgtcat taagaagaaa gtcagctggc
     1201 acgaagcgcg tgaaactttc gccaaccgtg gggagagcta caaagtctcc attcttgacg
     1261 aaaacatcgc ccatgatgac aagccaggtc tgtacttcca tgaagaatat gtcgatatgt
     1321 gccgcggtcc gcacgtaccg aacatgcgtt tctgccatca tttcaaacta atgaaaacgg
     1381 caggggctta ctggcgtggc gacagcaata acaaaatgtt gcaacgtatt tacggtacgg
     1441 cgtgggcaga caaaaaagcg cttaacgctt acctgcaacg cctggaagaa gccgcgaaac
     1501 gcgaccaccg taaaatcggt aaacagctcg acctgtacca tatgcaggaa gaagcgccgg
     1561 gtatggtatt ctggcacaac gacggctgga ccatcttccg tgaactggaa gtgtttgttc
     1621 gttctaaact gaaagagtac cagtatcagg aagttaaagg tccgttcatg atggaccgtg
     1681 tcctgtggga aaaaaccggt cactgggaca actacaaaga tgcaatgttc accacgtctt
     1741 ctgagaaccg tgaatactgc attaagccga tgaactgccc ggggcacgta caaattttca
     1801 accaggggct gaagtcttat cgcgatctgc cgctgcgtat ggccgagttt ggtagctgcc
     1861 accgtaacga gccgtcaggt tcgctgcatg gcctgatgcg cgtgcgtggt tttacccagg
     1921 atgacgcgca tatcttctgt actgaagaac aaattcgcga tgaagttaac ggatgtatcc
     1981 gtttagtcta tgatatgtac agcacttttg gcttcgagaa gatcgtcgtc aaactctcca
     2041 ctcgtcctga aaaacgtatt ggcagcgacg aaatgtggga tcgtgctgag gcggacctgg
     2101 cggttgcgct ggaagaaaac aacatcccgt ttgaatatca actgggtgag ggcgctttct
     2161 acggtccgaa aattgaattt accctgtatg actgcctcga tcgtgcatgg cagtgcggta
     2221 cagtacagct ggacttctcc ttgccgtctc gtctgagcgc ttcctatgtg ggcgaagaca
     2281 acgagcgtaa ggttccggta atgattcacc gcgcaattct ggggtcgatg gaacgtttta
     2341 tcggtatcct gaccgaagaa tttgctggtt tcttcccgac ctggcttgcg ccggttcagg
     2401 ttgttatcat gaatattacc gattcacagt ctgattacgt taacgaattg acgcaaaaac
     2461 tatcaaatgc gggcattcgt gttaaagcag acttgagaaa tgagaagatt ggctttaaaa
     2521 tccgcgagca cactttgcgt cgcgtcccat atatgctggt ctgtggtgat aaagaggtgg
     2581 aatcaggcaa agttgccgtt cgcacccgcc gtggtaaaga cctgggaagc atggacgtaa
     2641 atgaagtgat cgagaagctg caacaagaga ttcgcagccg cagtcttaaa caattggagg
     2701 aataaggtat taaaggcgga aaacgagttc aaacggcgcg ccctaaccgt atcaatggcg
     2761 aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg cgagcagctt ggtattgtga
     2821 gtctgagaga agctctggag aaagcagaag aagccggagt agacttagtc gagatcagcc
     2881 ctaacgccga gccgccggtt tgtcgtataa tggattacgg caaattcctc tatgaaaaga
     2941 gcaagtcttc taaggaacag aagaaaaagc aaaaagttat ccaggttaag gaaattaaat
     3001 tccgtcctgg tacagatgaa ggcgactatc aggtaaaact ccgcagcctg attcgctttc
     3061 tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg cggtcgtgag atggcgcacc
     3121 agcaaatcgg tatggaagtg cttaatcgcg tgaaagacga tttgcaagaa ctggcagtgg
     3181 tcgaatcctt cccaacgaag atcgaaggcc gccagatgat catggtgctc gctcctaaga
     3241 agaaacagta aggccttcaa gtagcactgt ctgtggagcc tttgggcttc acaggcattg
     3301 ttcgcctttg tttcgtttat taacaatgcg aagtggaagt tattaaaatg ccaaaaatta
     3361 agaccgtacg cggtgctgct aagcgcttca aaaaaaccgg taaaggtggt tttaagcaca
     3421 agcacgctaa cctgcgtcac attctgacca aaaaagcgac caaacgtaaa cgtcacctgc
     3481 gtccgaaagc catggtttcc aaaggcgatc tgggcctggt aatcgcgtgc ctgccgtacg
     3541 cataagccgt taacgttttt aactttttaa ttagaatata gatacaggag agcacatatg
     3601 gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca agaaaatttt gaaacaagct
     3661 aaaggctact acggtgcgcg ttctcgcgta taccgcgttg ccttccaggc tgttatcaaa
     3721 gctggtcagt atgcttaccg cgaccgtcgt caacgtaagc gtcagttccg tcaactgtgg
     3781 attgcgcgta tcaacgcagc agcacgtcag aacggtattt cttacagcaa attcatcaat
     3841 ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc tggctgatat cgcagtattc
     3901 gacaaagtag cgttcaccgc tctggttgaa aaagcgaaag cagctctggc ataagccagt
     3961 tgaaagaggg agctagtctc cctcttttcg tttcaacgcc atcaaaacat tgacttttat
     4021 cgccgtagcc ttttcaataa aggtcttttg aagagtacca aaaggtaacg caagcaatga
     4081 atgctgctat tttccgcttc tttttttact ttagcacctg actccaggag gctagcgcgt
     4141 gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt ggaggctttt tttgtatgcg
     4201 cgttttgaaa aataaaaagc ctgtctcatc aggcgtaatt gtcgcagcca gtctggacac
     4261 ggccagcgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4321 accaaaatgg caagtaaaat agcctgatgg gatgggctct aagtccaacg aaccagtgtc
     4381 accactgaca caatgaggaa aaacatgtca catctcgcag aactggttgc cagtgcgaag
     4441 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4501 ggtaaaaaag ggcacataac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4561 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4621 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4681 gatgtctccc tgccgggtcg tcgcattgaa aacggcgggc tgcatccggt gacccgtacc
     4741 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4801 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4861 gctgaccacg acactttttg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     4921 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgc
     4981 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5041 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5101 aacttctttg aggaagattt gcagatccgc ttccgtcctt cctacttccc gtttaccgaa
     5161 ccttctgcag aagtggacgt catggggaaa aacggtaaat ggctggaagt actgggctgc
     5221 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5281 ttcgccttcg ggatggggat ggagcgtctg accatgttgc gttacggcgt caccgacctg
     5341 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5401 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgacg
     5461 cgctggcaaa tcaaatcacg atggcgggcc tggaagttga tggtgtagaa ccggttgccg
     5521 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5581 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5641 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc gaccattggt gctgttctgc
     5701 cgggtgattt caaaattaaa gccgcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5761 gctccttctc tgagctggga atttctgacg atcataacgg cattatcgag ctgcctgcgg
     5821 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgacaac accatcgaaa
     5881 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgcgacgttg
     5941 ccgtgtcgaa ccagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6001 acgacacgct gccgattgca gtagaagcgc cggatgcctg cccgcgctat cttggccgtg
     6061 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6121 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgtg ctgctcgaac
     6181 tgggccagcc aatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6241 tggcgaaaga gggcgaaacg ctggtgttgc tcgacggcac tgaagcgaag ctggatgctg
     6301 acaccctggt catcgccgac cacaacaagg cgctggcgat gggcggcata tttggtggcg
     6361 aacactctgg cgtgaatgac gaaacgcaaa acgttctgct ggaatgcgct ttcttcagcc
     6421 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6481 atgagcgtgg cgttgatccg gcactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6541 tgatcgacat ctgcggtggt gaagcgggtc cggtaattga tatcactaac gaagcaacgc
     6601 tgccgaagcg tgcaaccatc actttacgtc gtagcaaact ggatcgcctg atcggccatc
     6661 atattgctga tgagcaggta acagacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6721 gcaaagacga gtggcaggca gttgcgccga gttggcgttt cgacatggag attgaagaag
     6781 atctggtcga agaagtcgcg cgtatttacg gctacaacaa catcccggat gagccggtac
     6841 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtaaaaa
     6901 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta tagcttcgtt gatccgaaag
     6961 tgcagcagat gatccatcct ggcgttgaag ccttactgct gccaagcccg atctctgttg
     7021 aaatgtcagc aatgcgtctt tccctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7081 agaaccgtca gcagaatcgt gtacgcattt tcgaaagcgg tctgcgcttc gtgccagata
     7141 ctcaggcacc gttgggtatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7201 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7261 atcttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7321 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7381 gttttgttgg ggttgttcat cctgaactgg aacgtaaact ggatctcaac ggtcgcactc
     7441 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7501 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7561 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7621 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7681 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7741 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7801 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7861 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     7921 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     7981 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8041 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8101 ctaactaaaa aggccgctct gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8161 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8221 gcgacaaaat tttcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8281 aagcctttgc gccggtgaac tatggatttt gccaggtgac tggttttctc ctcgtggcga
     8341 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8401 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8461 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8521 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8581 tttaatactc ttacgtttcg ctcgtcgtca tctttcgacc agtcggttat tgctggctgg
     8641 cgttgcatta gggattatct gtagcgcact aatgacgtgg gctatctact tttccacctc
     8701 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8761 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8821 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccctgtg
     8881 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     8941 ggcgggtgct atcggcttta ttggtctggt gatcccacat attctccggc tgtgtggttt
     9001 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9061 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgccaattg gcgtggtcac
     9121 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9181 aaagacggtc tatgattaaa agctaagatt tttactgacc acacccagga gaaacgatgc
     9241 aagattccat tctgacgacc gtagtgaaag atatcgacgg tgaagtgacc acgctggaga
     9301 agtacgccgg taatgtgctg ttgattgtca atgtcgcctc aaagtgtggc ttaacgccgc
     9361 aatatgagca gttggagaat attcagaaag cctgggctga tcgtggtttt gtggtgctgg
     9421 gattcccgtg taaccagttt ctggagcaag aaccgggtag cgatgaagag attaaaactt
     9481 actgcaccac cacatggggg gtgacgttcc cgatgttcag taagattgaa gttaatggcg
     9541 aaggacgcca tccgctgtat caaaaattga ttgccgcagc gccgactgca gtcgcgccgg
     9601 aagagagcgg attctatgcc cgtatggtca gcaaaggtcg tgcaccgctg taccctgatg
     9661 atattttatg gaattttgag aaatttttag ttggcaggga cggattggtc atccagcgtt
     9721 tttccccgga tatgacgccg gaagatccca ttgtgatgga aagcattaaa ctggcgttgg
     9781 caaaataatg tctattgtga tgcagttaca ggatgttgcg gaatctaccc gcctggggcc
     9841 gctttctggc gaggttcggg ctggggagat cctgcacctg gtggggccga atggcgcggg
     9901 taagagtacc ttactggcgc gaatggccgg aatgaccagc ggtaagggaa gcattcagtt
     9961 cgcggggcaa ccactggaag catggtctgc aacaaagctc gcgctgcatc gcgcctatct
    10021 ttcacaacag cagacgccgc cgtttgcaat gccggtctgg cactacctga cactgcatca
    10081 gcacgataaa acgcgtaccg aactactgaa tgatgtcgca ggggcgctgg ctcttgatga
    10141 caaactcgga cgtagcacca atcaactttc cggcggtgaa tggcaacgcg tacgtcttgc
    10201 tgcggtggtg ctgcaaatca caccacaagc caatcccgca ggccaattgc tgcttcttga
    10261 tgagccgatg aacagtcttg atgttgcgca acaaagcgcc ttagacaaaa ttctgagcgc
    10321 gctgtgtcag caaggactgg cgattgtgat gagcagtcac gatctcaacc acacattgcg
    10381 tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg ctggccagtg gacgcagaga
    10441 agaggtgctc acgccggcaa atctggcgca ggcctatggg atgaattttc gccgtctgga
    10501 tatcgaaggt cacagaatgc tgatttcgac catctgattt cattagtatc ttgaaaatcg
    10561 ggtaattacg acgctaaatt aatgcaagaa taaaaaacag aggaaccgta cggaatgcgt
    10621 ttctgcctta ttttgatcac agcactgttt ctggccgggt gtagccacca taaagcaccg
    10681 ccgccaaatg ccagactttc tgattcgatt accgttattg ccggtttgaa cgaccagcta
    10741 caaagctggc atggcacgcc gtatcgttat ggtggcatga cgcggcgcgg tgtggactgt
    10801 tcgggatttg tggttgtgac gatgcgcgat cgtttcgatt tgcagctgcc ccgtgaaacc
    10861 aagcaacaag cctctattgg cacgcaaatt gataaagacg agttgctgcc aggtgacctg
    10921 gtctttttca aaacgggttc cggacaaaat ggtttgcatg taggtattta cgataccaac
    10981 aaccaattta tccacgcctc taccagcaag ggagtgatgc gttcctcact tgataatgtc
    11041 tattggcaga aaaatttctg gcaggcgaga cgaatctagt atgtcgaaaa atggacggcg
    11101 atgatacatc gccgtccatt tactcctgaa tacagtagta ttttgcagac aaaaattccg
    11161 taaatggatt attcgtttat aatcaggata atctaaaaaa aggacccctg aaaaagtggt
    11221 taacatggaa caaatgaaag ttactccttc tgtaattgtt atgtcttatg gcctcatccc
    11281 ttttagccgg atactgaaaa acatccttcg agagggacgg ttaccatgaa gatttttttg
    11341 gagaatcttt atcattctga ttgctatttc ctgccgataa gagataacca gcaagacctt
    11401 gtcggcgttg agttaattac ccatttctcc agtgaagatg gtacggttcg tatcccgacg
    11461 agtcgggtta tagcgcaact taccgaggag caacattggc aattattttc tgaacagttg
    11521 gaattactaa aatcatgcca acactttttt attcaacata aactctttgc ctggcttaat
    11581 ttaacgccac aagtggctac attattatta gacagggata atttcgcagg cgaactatca
    11641 aaatatccct ttattgaact gttgattaat gagaattacc cgcatttcaa tgaaggaaaa
    11701 gataatcgcg atcttttatc cttatcgcaa atgtatcccc ttgtgttagg taatttgggt
    11761 gcaggtaata gcacaatgaa agcggttttt gatggtcttt tcacccgcgt catgctggat
    11821 aaaagtttta ttcagcaaca aatcacgcat cgttccttcg aaccgtttat tcgtgcgatc
    11881 caggcgcaaa tttccccttg ttgcaattgc atcatcgcgg ggggcattga tacgccagaa
    11941 atattggcac agattatccc ctttgatttc catgctctcc agggatgcct gtggcctgcg
    12001 gttcctataa atcagataac gacgttggtt cagcgataac cctcctgttt gctggtgttt
    12061 aggacgagag taaccgtcta cactatcaaa caggaggatc tatgaccctg tcttttatta
    12121 cccgctggcg cgatgaattg ccagaaacct atacagcact ttcccctacg cctttaaata
    12181 atgcccggct gatttggcat aataccgaac tggctaacac gctgagtatt ccatcgtcgc
    12241 tgtttaaaaa tggcgcaggt gtctggggcg gcgaaaccct actgcctggt atgtcaccac
    12301 tggcgcaggt ttacagtggt catcagtttg gcgtctgggc gggccaactg ggtgatgggc
    12361 gcggcatttt actcggcgaa caactgcttg ctgatggcac tacaatggac tggcatctga
    12421 aaggtgctgg cctgacgcct tattcgcgaa tgggtgatgg acgggcggtt ttacgttcga
    12481 cgatacgaga aagtctcgcc agtgaggcga tgcattatct gggcattccg acgacccgcg
    12541 ctttaagtat cgtcaccagc gattcgccag tgtatcggga aacggtggag tcaggcgcga
    12601 tgctgatgcg tgtggcacca agtcatctgc gctttggtca tttcgaacat ttttactatc
    12661 gccgcgagcc ggaaaaggtt cgtcagttag ctgactttgc cattcgtcat tactggtcac
    12721 atcttgatga tgaggaggac aaataccgtc tctggtttac cgatgttgtc gcacgtaccg
    12781 cctcgttaat tgcccaatgg cagacggtcg gctttgctca tggggtgatg aataccgaca
    12841 acatgtcgct gctggggctg acgcttgatt acgggccgtt tggttttctt gatgattacg
    12901 aacccggttt tatttgtaat cactcggatc atcaagggcg ttacagcttt gataatcaac
    12961 ctgccgtcgc gttgtggaat ttacagcgtc tggcgcagac attgtcgcca tttgttgccg
    13021 tagatggcct gaatgaggcc ctggacagct atcagcaggt tttgttgacg cattatggac
    13081 aacggatgcg gcagaaactg ggcttcatga cggagcaaaa agaggataac gcgctactta
    13141 atgaattatt cagtctgatg gcgcgagagc gcagcgatta tacccgcaca ttccgcatgc
    13201 tgagtctgac cgagcagcac agcgcggcgt caccgctacg tgatgagttt attgatcgtg
    13261 cggcatttga tgactggttt gcccgttatc gggggcgttt gcaacaagac gagattaccg
    13321 atagtgagcg tcagcaactg atgcaaagcg ttaacccggc tctggtgttg cgcaactggt
    13381 tggcgcaacg ggcgattgaa gcggcagaaa agggtgatat gacggaattg caccgcctgc
    13441 atgaggcgtt gcgaaatcct ttcagcgaca gagatgatga ctatgtcagt cgtccacccg
    13501 actggggtaa acggctggaa gtcagctgct cgagttaaag gttacttggt caacagcagc
    13561 ttgccagcct gagttttacg gagcagatat tcttgcccgt catgatcgat aatcagtttg
    13621 ccatccggac ctaacagtgt ctggctggta attcgccgga tgggctgcgg ggaagcggtc
    13681 ttgtgactg
//
LOCUS       DADTFG010000130        13514 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.131, whole genome shotgun sequence.
ACCESSION   DADTFG010000130 DADTFG010000000
VERSION     DADTFG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.131"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(351..1148)
                     /locus_tag="J1138_003588"
     CDS             complement(351..1148)
                     /locus_tag="J1138_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8455340.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFHAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSIFRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1158..1709)
                     /gene="ybcL"
                     /locus_tag="J1138_003589"
     CDS             complement(1158..1709)
                     /gene="ybcL"
                     /locus_tag="J1138_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBA8455341.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1878..2210)
                     /gene="emrE"
                     /locus_tag="J1138_003590"
     CDS             complement(1878..2210)
                     /gene="emrE"
                     /locus_tag="J1138_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBA8455342.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2555..2869)
                     /gene="fliE"
                     /locus_tag="J1138_003591"
     CDS             complement(2555..2869)
                     /gene="fliE"
                     /locus_tag="J1138_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBA8455343.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3084..4742
                     /gene="fliF"
                     /locus_tag="J1138_003592"
     CDS             3084..4742
                     /gene="fliF"
                     /locus_tag="J1138_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HBA8455344.1"
                     /translation="MNATAVQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLQVLLVAWLLWRKAVRPQLTRRAEAVKAVQQQAQTREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4735..5730
                     /gene="fliG"
                     /locus_tag="J1138_003593"
     CDS             4735..5730
                     /gene="fliG"
                     /locus_tag="J1138_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBA8455345.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5723..6409
                     /gene="fliH"
                     /locus_tag="J1138_003594"
     CDS             5723..6409
                     /gene="fliH"
                     /locus_tag="J1138_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBA8455346.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMAESEETIIEEVEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYLEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6409..7782
                     /gene="fliI"
                     /locus_tag="J1138_003595"
     CDS             6409..7782
                     /gene="fliI"
                     /locus_tag="J1138_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBA8455347.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLESYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7801..8244
                     /gene="fliJ"
                     /locus_tag="J1138_003596"
     CDS             7801..8244
                     /gene="fliJ"
                     /locus_tag="J1138_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBA8455348.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8241..9368
                     /gene="fliK"
                     /locus_tag="J1138_003597"
     CDS             8241..9368
                     /gene="fliK"
                     /locus_tag="J1138_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBA8455349.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9473..9937
                     /gene="fliL"
                     /locus_tag="J1138_003598"
     CDS             9473..9937
                     /gene="fliL"
                     /locus_tag="J1138_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBA8455350.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9942..10946
                     /gene="fliM"
                     /locus_tag="J1138_003599"
     CDS             9942..10946
                     /gene="fliM"
                     /locus_tag="J1138_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBA8455351.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10943..11356
                     /gene="fliN"
                     /locus_tag="J1138_003600"
     CDS             10943..11356
                     /gene="fliN"
                     /locus_tag="J1138_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBA8455352.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGALQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11359..11724
                     /gene="fliO"
                     /locus_tag="J1138_003601"
     CDS             11359..11724
                     /gene="fliO"
                     /locus_tag="J1138_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBA8455353.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEV
                     PQTDFQSVMKNLLKCGGRS"
     gene            11724..12461
                     /gene="fliP"
                     /locus_tag="J1138_003602"
     CDS             11724..12461
                     /gene="fliP"
                     /locus_tag="J1138_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBA8455354.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12471..12740
                     /gene="fliQ"
                     /locus_tag="J1138_003603"
     CDS             12471..12740
                     /gene="fliQ"
                     /locus_tag="J1138_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBA8455355.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12749..>13514
                     /gene="fliR"
                     /locus_tag="J1138_003604"
     CDS             12749..>13514
                     /gene="fliR"
                     /locus_tag="J1138_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBA8455356.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLIFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAELMPLIAPFCEHLFSEIFNLLADII"
BASE COUNT         3343 a         3480 c         3511 g         3180 t
ORIGIN      
        1 ccccattttt aataataccc agtgaacaat taacgtatca actagattta ataacaacga
       61 tatccgtacc taacctgata tctaatgtca taagtatccc tttaattatc ccctcaacct
      121 cttgtaactt tcccactctt gagctccaca tagtccaaac gccggatgca aaaaaatcac
      181 acgtgcattt ctgaaattgt gatggaggaa gatagtatca attatatgtg cttataaacg
      241 tccccgatta gcgcagaagt caccggattg ttcaggatac aataggttga gtatgccgta
      301 tttattttcc aaaataaaaa tcgatgctgt tgctccttca taaaatactt ttactctctg
      361 gacgcttttc ttagctctct tggtgttatc ttaaaacgat tatggaacct ttcagtaaaa
      421 cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt tactgtcgtt
      481 tgtatagcag acaacgcatt atttaacctc acgtcgtcca atatactttg aaaacttatt
      541 ccctcgcttg ccagacggcg atgcaatgtg gaaacagaaa tgtagagata tctcgctacc
      601 ttgtttgctg tccattttgt tccaggttcg gatagcatta ggttataaca acgacttatt
      661 aatgattgtt tactatctga taaaagtaaa caattaacat gatgtgctcc taatgaaagt
      721 agaacgccca ttgctaagtg ttcctgaatt tgcgttgaga agcctcggaa aatagatgtt
      781 tttagttgct cccaacaata tattaactcg gggttctgag gtaagcagaa acctgtcgtg
      841 ttacgaattt gatcagttat cgcatgaaat ttttgaaact tttcaattag atcaatggga
      901 taataaagca tttctgcaag ataaagacct gcttctggat aattcgcaat ataaaattca
      961 tatccacagg gaaataatat tatctgatta ttatcaacag ttagactatg cgtctcccaa
     1021 ttgataactt tttttccctg acggatacga cataaagctg gcataagagg cttaaccctg
     1081 tgaatctcat gatgtttatg catccgtatt tcttcaatct ttaggttaga cttatctttt
     1141 gccagcatac tcttacccta ctttatctca taaactggtg ttatctcagc ggttgcaatt
     1201 ttattggcat taagcatata accaactaac gctccgctgg aattagaatc cacaggaatc
     1261 ttatcagttt ttagagccca tactttaaac tggtaatgat ggggtttatc tcctttggga
     1321 ggacatgctc caccaaatcc tgcataacca aaatcatttc ggccttgaac agcaccagtc
     1381 ggaagttttg taccatcacg tcttcctgca tcagcgggca aatatgttac agttgctgga
     1441 atattagcaa cagtccagtg ccaccatcca ctgcctgtag gagcatctgg atcatacaca
     1501 gttacggcaa agcttttggt gccttcagga gcaccagacc aggttaatga gggcgatgta
     1561 ttaccccctt cacacccaaa tccagaaaat acatgagacg ttgtaagttg ctctcctgtt
     1621 tttatttcat tactagtgac ctgaaatgct gcggcctgcg cagaaaatgt tatgaatgcc
     1681 aatacacttg aaacgataag ttttttcata aaaacctcat tgttgtgacc tatcgttatt
     1741 ttatttgatg tgccttgatc tcattatgca taaaggcgca atgatcgcgc aaatgcaatc
     1801 ataattataa aggtgccccg aacattagcc ataatatgca caaatcagat tcctgttatc
     1861 tcaaggataa ttatttttta atgcggtgca cttcgtgaca acaagttaat gactaacaca
     1921 ccggcacaaa tcaacatcat gcctatgata gctggcaggt ccagtcgttg accaaaaaat
     1981 ccccaggaaa gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc
     2041 cctgtaggta tataagccag cgtctgagct aataaccaga atgatgcaca atagcaaata
     2101 attgtgccaa cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta
     2161 ccaatgacct ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct
     2221 ttcctgttca tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc
     2281 tggtgtatgt gtatccaggg ggctctggcg tggcaagaga taaaggcagg aacaataatg
     2341 atcagaagga accccaggct aacgaatgag ttcactcttc ctgggagcaa agcgaagaca
     2401 aaacatgtta tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat caggtatcca
     2461 ttttaaacca gaaattgagt gagtttgccc catccatgcc ccacgctgtg ggatcggata
     2521 taattaaaca agcatagtca acaggttaca caacctacac ctgcatggac atcacttcct
     2581 gatacgccgc caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt
     2641 tttgcatatc ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt
     2701 ctgcctgcgt gcgggcggct gtttgtgtat cgcttatgcg atcgagcgcg gcgtgcagct
     2761 gcccggcaaa actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg
     2821 tagcctgtaa ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc
     2881 ggataatttc tggtagcaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca
     2941 acaaaaaaac gggtttattg gcggatagaa aaaaacgaaa gcacaaataa tgggagcgtc
     3001 aatttttcga gtttgctgac ccgggagtga gtcttgttcc actttgccaa taacgccgtc
     3061 cataatcagc cacgaggtgc gcgatgaatg cgactgcagt ccagacaaaa tctcttgagt
     3121 ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat tgttgccggt tccgcagcag
     3181 tggcggtcat ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc acattattca
     3241 gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc
     3301 cttaccgctt cagcgaagcc agcggcgcta ttgaagttcc ggcagataaa gttcacgaac
     3361 tgcgcctgcg cctggcgcaa caaggtttgc caaaaggcgg cgcggtcggt ttcgaactgc
     3421 tcgatcagga aaagtttggt atcagccagt tcagcgaaca ggtgaattat cagcgggcgc
     3481 tggaaggcga gctttctcgt accatcgaaa ctatcggccc ggtaaaaggg gcgcgcgtac
     3541 atctggcaat gccgaaaccg tctttattcg tccgtgaaca aaaatcccct tctgcatcgg
     3601 tgacggtaaa tctgttaccc ggccgcgcac tcgatgaagg gcaaattagc gccattgtac
     3661 atctggtttc cagcgcggtg gctggtctgc cgccgggaaa cgtcacgctg gtggatcagg
     3721 gcggacatct gttaacccag tccaacacca gcgggcgcga tcttaatgac gctcagttga
     3781 aatatgccag cgatgtcgaa ggccgtattc agcggcgtat tgaagcgatc ctgtcgccta
     3841 ttgttggtaa cggtaatatt cacgcccagg tcacggcgca gctggacttc gccagtaaag
     3901 aacaaacgga agaacagtat cgccctaacg gtgatgaatc tcaggcggcg cttcgttcac
     3961 gccagcttaa tgagagcgag caaagcggtt ccggttatcc gggcggcgta ccgggggcgt
     4021 tgtcgaatca accggcacct gcgaataacg cgccaatcag cacgcctccg gcaaatcaaa
     4081 ataaccgcca gcagcaggcg agcaccacca gcaatagtgg gccgcgtagc acacagcgta
     4141 atgaaaccag taactacgaa gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg
     4201 atgtgcaacg tctgtcagtc gcggtagtgg tgaattacaa aaccttgcca gacggtaaac
     4261 cgttgcctct cagcaacgaa cagatgaagc aaattgaaga tctgacccgc gaggcgatgg
     4321 gcttttctga aaaacgcggt gactcgctca atgtcgtcaa ctcgccgttc aatagcagtg
     4381 acgaaagcgg cggagaactg ccgttctggc aacagcaagc gtttatcgat cagttacttg
     4441 ctgccggtcg ctggttgcag gtactgctgg tggcgtggct gctgtggcgg aaagcggtac
     4501 gcccgcagct aacacgtcgc gccgaggcgg tgaaggctgt gcagcaacag gcgcagaccc
     4561 gcgaggaagt ggaagatgcg gtggaagtcc gcctgagcaa agacgaacaa ctccaacaac
     4621 ggcgcgctaa ccaacgactg ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg
     4681 ataacgatcc gcgcgtggtg gcgctggtca ttcgccagtg gataaataac gatcatgagt
     4741 aacctgacag gcaccgataa aagcgtcatc ctgctgatga ccattggcga agaccgggcg
     4801 gcagaggtgt tcaagcacct ctcccagcgc gaagtgcaaa ccctgagcgc tgcaatggcg
     4861 aacgtcacgc agatctccaa caagcagcta accgatgtgc tggcggagtt tgagcaagaa
     4921 gctgaacagt ttgctgcact gaatatcaac gccaacgatt atctgcgttc ggtattggtc
     4981 aaagctctgg gtgaagaacg tgccgccagc ctgctggaag atattctcga aacccgcgat
     5041 accgccagcg gtattgaaac gctcaacttt atggagccgc agagcgccgc cgatctgatt
     5101 cgcgatgagc atccgcaaat tatcgccacc attctggtgc atctgaagcg cgcccaggcc
     5161 gccgatattc tggcgttgtt cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc
     5221 acctttggcg gcgtgcagcc agccgcgctg gccgaactga ccgaagtact gaatggcttg
     5281 ctcgacggtc agaatctcaa gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt
     5341 atcaacctga tgaaaacgca gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac
     5401 ggcgagctgg cgcagaaaat catcgacgag atgttcctgt tcgagaatct ggtggatgtc
     5461 gatgatcgca gcattcagcg tctgttgcag gaagtggatt ccgaatcgct gctgatcgcg
     5521 ctgaaagggg ccgagcagcc actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc
     5581 gccgatattc tgcgcgacga tctcgccaac cgtggtccgg tgcgtctgtc gcaggtggaa
     5641 aacgaacaga aagcgattct gctgattgtg cgccgtcttg ccgaaactgg cgagatggtg
     5701 attggcagcg gcgaggatac ctatgtctga taatctgccg tggaaaacct ggacgccaga
     5761 cgatctcgcg ccaccaccgg cagagtttgt gccaatggcc gagtcggaag aaaccattat
     5821 tgaagaggtc gaacccagcc ttgagcagca actggcgcaa ctgcaaatgc aggcccacga
     5881 gcagggttat caggcgggga ttgccgaagg tcgccagcaa ggtcacgaac agggctatct
     5941 ggaaggactg gcccaggggc tggagcaagg tctggcagag gcgaaggctc aacaagcgcc
     6001 aattcatgcc cggatgcagc aactggtcag cgaatttcaa actacccttg atgcgcttga
     6061 tagtgtgata gcttcgcgcc tgatgcagat ggcgctggag gcagcacgtc aggtcatagg
     6121 tcagacgcca acggtggata actcggcact gatcaaacag atccaacagt tgttgcagca
     6181 agaaccgtta ttcagcggga aaccacagct gcgcgtacac ccggatgatc tgcaacgtgt
     6241 ggatgatatg ctcggcgcaa ctttaagttt gcatggctgg cgcttgcggg gcgatcccac
     6301 cctccatcct ggcggctgta aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc
     6361 cacccgctgg caagaactct gccgtctggc agcaccagga gtggtgtaat gaccacgcgc
     6421 ctgactcgct ggctaaccac gctggataac tttgaagcca aaatggcgca gttgcctgcg
     6481 gtacgtcgct acgggcgatt aacccgcgct accgggctgg tgctggaagc caccggatta
     6541 caattgccgc tcggcgcaac ctgtgtcatt gagcgccaga acggcagcga aacgcacgaa
     6601 gtagaaagcg aagtcgttgg ctttaacggt caacggctgt ttttaatgcc gctggaggaa
     6661 gtggaaggtg tcctgcccgg cgcgcgtgta tatgccaaaa acatttcggc agaagggctg
     6721 caaagcggca agcagttgcc gctcggtccg gcgttattag gtcgtgttct ggacggtagc
     6781 ggtaaaccgc tcgatggcct gcccttcccc gatacgacgg aaaccggtgc gctgattacc
     6841 ccgccattta acccgttgca acgtacaccg attgaacatg tgctggacac cggcgtgcgc
     6901 ccaatcaatg ccctgcttac cgtcggacgc gggcagcgta tggggctgtt tgccgggtcc
     6961 ggcgttggga aaagtgtgct gctggggatg atggcacgtt acacccgcgc cgatgtcatt
     7021 gtcgtgggtt tgattggtga acgtgggcgc gaagtaaaag attttattga gaatatcctc
     7081 ggtgccgaag gacgtgcacg ctcagtggtg attgccgctc cggcggatgt ttctccgctc
     7141 ctgcgaatgc agggtgccgc ctatgccacg cgcattgccg aagattttcg cgatcgtggt
     7201 cagcatgtgt tgctgattat ggactccctc acccgctacg cgatggccca gcgtgaaatt
     7261 gcgctggcga ttggcgaacc acctgccact aaaggttatc caccgtcggt gtttgccaaa
     7321 ttaccggcac tggtcgagcg tgccggaaat ggtattagcg gcggcggctc gattaccgcg
     7381 ttttataccg tgctcaccga aggcgatgac cagcaggacc ccattgctga ctccgcgcgg
     7441 gcaatcctcg acggtcacat tgtgctgtct cgccgactgg cggaagccgg gcactatccg
     7501 gctatcgata ttgaagcgtc gatcagtcgc gcaatgacgg cgttgatcag tgagcaacat
     7561 tacgcgcgag tgcgcacctt caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg
     7621 gttagcgtcg gcgcgtatgc caaaggcagc gatccgatgc tcgacaaagc catcgccctg
     7681 tggccgcagc tggagagcta tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg
     7741 tctctccagg ggctggagcg tattttcccg acagtgtcat aacccaggag ataacggcag
     7801 atggcggaac atggtgcgct ggcgaccctg aaagatctgg cagaaaaaga ggtagaggat
     7861 gccgctcgcc tgctgggtga aatgcgtcgc ggatgtcagc aggcggaaga acagctcaaa
     7921 atgctgatcg attatcagaa tgaatatcgc aataacctca atagcgatat gagtgccggg
     7981 atgaccagca accgctggat caactatcag cagtttatcc agacgctgga aaaagccatc
     8041 actcagcatc gccagcaact taatcagtgg acgcagaaag ttgacattgc cctgaacagc
     8101 tggcgagaaa agaaacaacg tttgcaggcc tggcagacac tgcaggaacg gcaatccacg
     8161 gcggcactgc ttgcagaaaa ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc
     8221 gccgccatga ggaaacctga atgattcgct tagcgccctt gattaccgcc gacgttgaca
     8281 ccaccacatt gcctggcggc aaagccagcg atgctgcaca agattttctc gcgttgttga
     8341 gcgaagcatt agcaggcgag acaacaaccg acaaagcggc cccccagttg ctggtggcaa
     8401 cagataagcc cacgacaaaa ggcgaaccgc tggtcagcga tattgtttct gatgcgcaac
     8461 aagcggattt actgattcct gtggatgaaa cacttcctgt catcaacgac gaacaatcca
     8521 catcaacacc gttaaccacc gctcagacga tgacgttggc tgcggtggct gacaaaaata
     8581 cgacaaaaga cgaaaaagcg gatgacctta atgaagacgt caccgcaagt ctgagcgccc
     8641 tttttgcgat gttgccgggt tttgacaata cgcccaaagt gactgatgca ccgtcaaccg
     8701 tgttaccggc agagaaacca acgctcttca caaaactgac ttctgcgcaa ctcactacag
     8761 cacagcctga tgacgccccc ggcacaccag ctcagccatt aacaccgctg gtagcagaag
     8821 cccagagtaa agcggaagtc atcagcacgc cttcgccggt gaccgctgac gccagcccgc
     8881 taatcactcc acaccagaca cagccactgc ccaccgtcgc cgcgcctgtt ttgagtgcac
     8941 cgctgggttc tcacgaatgg caacaatcat taagccagca tatttcgctg ttcacccgcc
     9001 aggggcaaca aagtgcagag ttgcgtctgc acccacagga tttaggtgaa gtgcaaattt
     9061 ccctcaaagt ggatgataac caggcgcaga tccagatgat ttcaccgcat cagcacgtac
     9121 gcgccgccct ggaagcagcg ctgccggtac tgcgtacgca gctggccgaa agtggcattc
     9181 agttagggca aagcaacatc agtggcgaaa gctttagtgg tcagcagcag gccgcttccc
     9241 agcaacagca aagccaacgc acagcaaacc atgaacctct ggcgggggaa gacgacgata
     9301 cgcttccggt tcccgtctct ttacaagggc gtgtaacagg taacagcggc gttgatattt
     9361 tcgcctaacg tcagaggtag caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa
     9421 gacgcaggat aattagccga taagcagtag cgacacagga agaccgcaac acatgactga
     9481 ttacgcgata agcaagaaaa gcaagcgatc gctttggatc ccgattctgg tattcattac
     9541 cctcgcggcc tgtgccagcg caggttacag ctactggcat tcgcatcagg ttgccgctga
     9601 cgacaaagcg cagcaacgcg tcgtgccctc cccggtcttc tatgcgctgg atacctttac
     9661 ggtcaatttg ggcgatgcgg atcgcgtgct ttatatcggc ataaccctgc gactgaaaga
     9721 tgaagctacc cgctcgcggc tgagtgagta tttgccggaa gtccgtagcc gtttgctgtt
     9781 actgttttcg cgtcaggatg ctgccgtact ggcgacagaa gaaggcaaga aaaacctgat
     9841 tgccgagatt aaaaccacac tttccacccc gcttgttgcc gggcaaccga aacaggatgt
     9901 caccgacgtg ctgtataccg cttttattct gcgataacga catgggcgat agtattcttt
     9961 ctcaagctga aattgatgcg ctgttgaatg gcgacagcga agtcaaagac gaaccgacag
    10021 ccagtgttag cggcgaaagt gacattcgtc cgtacgatcc gaatacccaa cgacgggttg
    10081 tgcgcgaacg tttgcaggcg ctggaaatca ttaatgagcg ctttgctcgc cattttcgta
    10141 tggggctgtt caacctgctg cgccgtagcc cggatattac cgtcggggcc atccgcattc
    10201 agccgtacca tgaatttgcc cgcaacctgc cggtgccgac caacctgaac cttattcatc
    10261 tgaaaccgct gcgcggcaca gggctggtgg tgttctcgcc gagtctggtg tttatcgccg
    10321 tggataacct gtttggcggc gatggacgct tcccgaccaa agtggaaggc cgcgagttta
    10381 cccataccga acagcgcgtc atcaaccgca tgttgaaact ggcgcttgaa ggctatagcg
    10441 acgcatggaa ggcgattaat ccgctggaag ttgagtacgt gcgttcggaa atgcaggtga
    10501 aatttaccaa tatcaccacc tcgccaaacg acattgtggt taacacgccg ttccatgtgg
    10561 agattggcaa cctgaccggc gaatttaata tctgcctgcc attcagcatg atcgagccgc
    10621 tacgggaatt gttggttaac ccgccgctgg aaaactcgcg taatgaagat cagaactggc
    10681 gcgataacct ggtgcgccag gtgcagcatt cacagctgga actggtggcc aactttgccg
    10741 atatctcgct acgcctgtcg cagattttaa aactgaaacc cggcgacgtc ctgccgatag
    10801 aaaaacccga tcgcatcatc gcccatgttg acggcgtccc ggttctgacc agtcagtacg
    10861 gcactctcaa cggtcagtat gcgttacgga tagaacattt gattaacccg attttaaatt
    10921 ctctgaacga ggaacagccc aaatgagtga catgaataat ccggccgatg acaacaacgg
    10981 cgcaatggac gatctgtggg ctgaagcgtt gagcgagcaa aaatcaacca gcggaaaaag
    11041 cgctgccgac gcggtgttcc agcaatttgg cggtggtgat gtcagcgggg cgttgcagga
    11101 tatcgacctg attatggata ttccggtcaa gctgaccgtc gagctgggcc gtacgcggat
    11161 gaccatcaaa gagctgttgc gtctgacgca agggtcagtc gtggcgctgg acggtctggc
    11221 gggcgaaccg ctggatattc tgatcaacgg ttatttaatc gcccagggcg aagtggtggt
    11281 tgttgccgat aaatatggcg tgcggatcac cgatatcatt accccgtccg agcggatgcg
    11341 ccgcctgagc cgttagtgat gaataaccat gctactgtgc aatcttccgc gccggtttct
    11401 gctgagccac tgctgcaggt gagcggcgca ctcatcgcca ttattgccct gatcctcgct
    11461 gctgcctggc tggtaaaacg gctgggattt gcccctaaac gcactggcgt taacggtctg
    11521 aaaattagcg ccagtgcttc gctgggcgcg cgtgaacggg tcgtggtggt cgatgtggaa
    11581 gatgcacggc tggtgctcgg cgttaccgca gggcaaatca atctgctgca taaacttccc
    11641 ccttcagcac caacggaaga ggtaccgcaa accgattttc agtcggtcat gaaaaatttg
    11701 cttaagtgtg gcgggagatc ctgatgcgtc gtttgttttc tgtcgcaccg gtccttctct
    11761 ggctgattac gcccctcgcc ttcgcgcaac tgccgggtat caccagccag ccgctacctg
    11821 gcggtggaca aagctggtcg ctcccggtgc agacgctggt gttcatcacc tcgttgacgt
    11881 tcattccagc gattttactg atgatgacca gtttcacccg catcattatt gtttttggtt
    11941 tattgcgtaa cgcgctggga acgccctccg cgccacctaa ccaggtattg ctggggctgg
    12001 cactgttttt gacctttttt attatgtcac cggtgatcga caaaatttat gtcgatgcgt
    12061 accagccatt cagcgaagag aaaatctcaa tgcaggaggc gctggaaaaa ggggcgcagc
    12121 cgctgcgtga atttatgctg cgtcagaccc gcgaggcgga tttagggttg tttgccagac
    12181 tggcgaatac cggcccgttg cagggacctg aagctgtgcc gatgcgcatt ttgctcccgg
    12241 cctacgtgac cagcgagttg aaaactgcat ttcagatagg cttcacgatt ttcatccctt
    12301 ttttgattat cgacctggtg attgccagcg tgttgatggc attggggatg atgatggttc
    12361 cccctgccac cattgctctg ccctttaaac tgatgctgtt tgtactggtg gatggctggc
    12421 aattgctggt cggttcgctg gcgcagagct tttacagcta gagaggcaaa atgacacctg
    12481 aatcggtcat gatgatggga actgaagcga tgaaagtcgc gctggcactg gcagccccgc
    12541 tgttgctggt ggcgctggtc acgggcctta tcatcagtat tttgcaggca gccacgcaga
    12601 ttaacgaaat gacgctgtcg tttattccga aaatcatcgc cgtatttatc gccattatta
    12661 ttgccggacc gtggatgctc aatctgttgc tggattacgt ccgcaccttg ttcactaacc
    12721 tgccgtatat catcgggtag cccgtacaat gatgcaggtg acaagcgatc aatggctttc
    12781 ctggttaagc ctgtacttct ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc
    12841 aattctgagc gaacgcagcg tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac
    12901 gttcgccatt gccccatcat tacctgccaa cgatgttcct gttttttcgt tctttgctct
    12961 gtggctggcc gtgcagcaga tcctgatcgg cattgcgctt ggttttacca tgcaatttgc
    13021 ctttgccgct gtgcgaaccg ctggcgaaat tatcggtctg caaatggggc tgtcatttgc
    13081 gacatttgtc gatcctggca gccatctcaa tatgcccgtt ttagcgcgca tcatggatat
    13141 gctggcatta ctgctgttcc tgatatttaa cggtcattta tggttgattt cactgctggt
    13201 cgataccttt cacaccctgc cgattggtgg tgaaccgttg aacagcaatg cgtttctggc
    13261 acttaccaaa gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat
    13321 tactctgttg ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt
    13381 atccattttt gttattggat ttccattaac tctgactgtc ggcatctctt taatggcgga
    13441 attaatgccg ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct
    13501 ggctgatatt atta
//
LOCUS       DADTFG010000131        13512 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.132, whole genome shotgun sequence.
ACCESSION   DADTFG010000131 DADTFG010000000
VERSION     DADTFG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.132"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..442
                     /locus_tag="J1138_003605"
     CDS             <1..442
                     /locus_tag="J1138_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBA8455357.1"
                     /translation="GFAWGAGFLDGLGEGAGRAAQPTVGLGATASMGAGDIAKSAIGG
                     GASKAASTLSDYYIKRAEQYHPVIPIGAGNEVTVVFQEGFQMKTIEEVAAEKESRQQG
                     QTEGSDQPKSGQSLNGFNTDQMLNHLGKLEPAQFGLGTSTTGER"
     gene            447..665
                     /locus_tag="J1138_003606"
     CDS             447..665
                     /locus_tag="J1138_003606"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455358.1"
                     /translation="MNNNDKPNIWYFDDFPFMGVASAEIGNRVVCGRTGAGKTTLMQS
                     IVDKYSADAVRQGISIAPKPPSEDEAKS"
     gene            662..1048
                     /locus_tag="J1138_003607"
     CDS             662..1048
                     /locus_tag="J1138_003607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455359.1"
                     /translation="MTERKMTGLYVFLLLAWWGGWLALDMWRIEHLLTDADKWAGFRR
                     DILWCAGSAVVSVILFIMTADKKLAVYVLLGFLAAAVGISTAFVVSDAFIHWGFDMSL
                     FSMAISVVGIATVPMLPFAAFLVARS"
     gene            1073..1612
                     /gene="traV"
                     /locus_tag="J1138_003608"
     CDS             1073..1612
                     /gene="traV"
                     /locus_tag="J1138_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBA8455360.1"
                     /translation="MKSLLLLPALGGVLLLAGCAGVSSEFGCNATTSDSCMTMEQANE
                     KAKSLEEGSEAKPAAAALPRLADGDFAAVGRDIGPVPVSVGSTSRLLPRQRAASSAPV
                     VPAYPAIAAPSAIVTTVSTCTLPRCAGVGSTRPARQAEQTAQLWIAPWIDSQDVYHQP
                     GKVLFVVKPAVWGQPQSVY"
     gene            1625..4255
                     /gene="traC"
                     /locus_tag="J1138_003609"
     CDS             1625..4255
                     /gene="traC"
                     /locus_tag="J1138_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBA8455361.1"
                     /translation="MNLLDKVTHTANSLLSALKMPDEAAAANKVLGDMNFPQLSSVLP
                     YRDYDEESGLFINAGSIGFVLEALPLAGANEQVVLTLESLLRTKLPRGIALSVHLMAS
                     KRVGDLVESGLRDFSWSGKDADKFNAITRAYYLMAAADRFSLPPGVDMPLTLRNYRVF
                     ISYCVKSKRRNKALITELENLVKVLRASLMGAKIPTESLNDEDFINVVGEIINHDPSQ
                     LYNKRRKLDPLKDLNFQCIDNGFGMDVYPDCVKINLRDAGSKKGSVARVMNFQLEQNP
                     EMAFLWNTADNYGNLLYPELSIATPFIITLTLVVEEQTKTSFEANRKYIDLEKKSRTS
                     YAKFFPGTLEEMKEWGDLRQSLASNQTAIVSFFYNVTVFCEDNDSVALRCEQDVINSY
                     RKNGFDLISPRFKQHWNFLACLPFMAEHGVFQDLAMHGAVHRAKTLNVVNLMPIVADN
                     RLAPSGLLAPTYRNQLAFIDIYSEVMRNTNYNMAVAGTSGAGKTGLIQPMLRSVLDTG
                     GAAWVFDMGDGYKSLCENMGGVYIDAQTLKFNPFANVKDINLSATQIANLISVMASPD
                     GNLDEVHESLLEMAVKAAWLSKQKNARIDDVRDFLRDAMDEEVYRDSPTIRNRLHEMT
                     ILLDKYCSDGTYGEFFNSDEPSLRDDVNMVVLEMGGLKNRPDLLVAVMFSLMIYIEGR
                     MYQTSRQSKKLCVIDEGWKLLNFKNAKAGEFIEEGYRTARRHRGAYITITQNIKDFDS
                     DTASSAAKAAWGNSSYKIILMQDASEFKTYLQKNPDNFTQLQQDMIGKFESAKDQWFS
                     SFMLMVSGKVSWHRLFVDPLSRAMYSSQGQDFEFMQARREQGVSVHDAVYELAHRNFG
                     EEMARIERWVEENYTGRAAA"
     gene            4252..4671
                     /gene="trbI"
                     /locus_tag="J1138_003610"
     CDS             4252..4671
                     /gene="trbI"
                     /locus_tag="J1138_003610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBA8455362.1"
                     /translation="MTLPAIITPPNTPAQKRAIVLWGTVCVLIAVLLSVGATLWLLQR
                     QPAIVTFDMKQTMEAFYESAAKQSLPPERSKLLAERFTGALQTSIAQYQQRNNVIILV
                     SPAVVGGARDVTRELQRDIAARMQRGNIVSVGEETNL"
     gene            4668..5522
                     /locus_tag="J1138_003611"
     CDS             4668..5522
                     /locus_tag="J1138_003611"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013488.1,HMM:NF036765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBA8455363.1"
                     /translation="MNSSAISFMAGFVVCFVIMFVTNLGRNQMPATITATPVVFTPAQ
                     QDAIGPLARDYLLAHPDILLQMSEKLEAQQQTAANRTVQTALTTQLELQATLLHDPAT
                     PVVHPDGGVTVVQFFDYQCIWCARMAPVVADFVRDNPDVRFVFKEFPIFGSRWPMSVQ
                     AARTGLTLWQLKGGEAYLAYHNAVFASGENEGRLSQPTLNAALQRAGAAPMTVLPETE
                     KTLLATRELAQQLNISGTPAFIVMPTRDARADQVTPLYGAVDVKALKAAVAQAKQADI
                     PSQAVPHG"
     gene            5515..5940
                     /locus_tag="J1138_003612"
     CDS             5515..5940
                     /locus_tag="J1138_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019453530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA8455364.1"
                     /translation="MAKLTKAQRAVLREKFGGRCAYCGCELPEKGWHADHLEPVMRES
                     EQDMAAAEKGLFKLKATGKVLHESRDAIENLNPACAPCNLFKTTFSLETFREQIAAQA
                     ERARAYSVNFRTAERFGLIEVKPAPVVFWFERYQEAERG"
     gene            5996..6586
                     /gene="traW"
                     /locus_tag="J1138_003613"
     CDS             5996..6586
                     /gene="traW"
                     /locus_tag="J1138_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBA8455365.1"
                     /translation="MAVAKDFGTWGDLYPVAEPDMLQTIHERLTALQRSGRWDEEMTA
                     FKERTVKHSQRPEPVAGIGKTERYAERFFDPSVQLSSDLKDDKGRVFARKGDVINPLA
                     TVPFAQTLYFIDGDDPQQVAWMKAQQPETLMVKVILVNGDIPKTSAALDSRIYFDQGG
                     ALSRRFGLTTVPARITAAPSGLRLRIESIPAAGSAN"
     gene            6598..6975
                     /locus_tag="J1138_003614"
     CDS             6598..6975
                     /locus_tag="J1138_003614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455366.1"
                     /translation="MFVTRKRFIATENQLQSCRQLLAARHGELMAIRQAASAKGGIYK
                     CILECREVAHELREQLPADQDGHCPYLFDKLALIDSLLGTLAALLPPQDEARPTARMV
                     AAGDVQEIYRGTQWATHCLGRRV"
     gene            6972..7970
                     /gene="traU"
                     /locus_tag="J1138_003615"
     CDS             6972..7970
                     /gene="traU"
                     /locus_tag="J1138_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBA8455367.1"
                     /translation="MTRCWLSALLVVAGLFSAPSQAADAACQGRFVNPITDICWRCIF
                     PLSIGSIPVGKGDLPDTSNPGSPIQFCPAPPPIFQRIGMAMGYWEPIAMTDVTRSPGC
                     MVNLGSFSINLGKIGTGTGGSSGKGDVTTAFYHVHWYKYPLTYWLNIITSVGCLQGGD
                     LDIAYLSEIDPTWVDSSLTTILNPEAVVFANPLAQGACAADAIASGFHLPIDALFWCA
                     GSQGSMYPFNGWVAKEISPLQSSVLVSEKMAFKLHRQGQIMESIGKDRAVCFEYPSPI
                     MPKERWRYQMVNMYPDSARCHPMGRSVMRWEVGHNSPASKKNFGYVMWRKRNCVFL"
     gene            8044..8655
                     /gene="trbC"
                     /locus_tag="J1138_003616"
     CDS             8044..8655
                     /gene="trbC"
                     /locus_tag="J1138_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011412250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBA8455368.1"
                     /translation="MIAVGQMAADPVATAQQQATVQVDSNRAWLREQEKRSADLHQNT
                     PVPEFLRNQPPRSLAQDDQSFIDQLAAKQRQDAVEQPAEGALYFVSFAIPEEGMRRML
                     QEARRFGIPATLRGMVNGDMRETVQRVQKLVEAGGIDGVQIDPQPYTQYGVTAVPALV
                     VRCAAGFDVVRGNLLMAQALKKVAKDGDCAATAQALLDKESGK"
     gene            8652..10556
                     /gene="traN"
                     /locus_tag="J1138_003617"
     CDS             8652..10556
                     /gene="traN"
                     /locus_tag="J1138_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBA8455369.1"
                     /translation="MTHLVKSLPGLLATHALVLGTFIFATGDCRAEGDDVFQTGSEFA
                     KQIAGQGTGTLQNTNPANVLPNYTDNPKEKGYYGGVTAGDANNLKTDGGTAWGQTEVG
                     QAVTDSVLKNPKEPISHDAPFIQAGKEIEGKAESIVGKTGPDCKAEQVTRSEFTRHVC
                     ERDTAVMRTCERATDIGGHYETRYEDRNAHIDKSMIRWWKEGNRIHGTFTAPFTGKLT
                     TGSIYLELQDDLHSYGVPLAFSLLGNEVRYGRQDWGNRPLNVGNVTLTQGQQVDIFLT
                     MDPGGHLDTFLDAMVRQLSGGGGGSPMVFYVKFDGSSSVQVWVPTTSTPADCPLAQEA
                     GAVKVNTVCSEPGGERTVTVNGQTYPVWASCWRFTDTYRLQEAGQGSCEAYANNPACT
                     IATHGCAFSDEYGQCLHENVTYSCETRTTGNVMLCGGQMYCLDGECDKAQKGTQGNDF
                     AHAVSELAAVAAAGKDVAALNGIDVRAFTGKGQSCRKAFAGFSDCCKDSGWGNSVGLA
                     HCDSEEKALGKAKENKLTVSVGEYCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRAW
                     QLGIGFGDAKSPDCRGITVDELQRINFDQLDFANFYEDLMKNQKVPEDAALLEKVKQQ
                     IAQRMQEAAK"
     gene            10553..11347
                     /gene="traF"
                     /locus_tag="J1138_003618"
     CDS             10553..11347
                     /gene="traF"
                     /locus_tag="J1138_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBA8455370.1"
                     /translation="MKTRGTSNVNLAARGLANRRAAALLGLSLLGAAFTAQAGGWQWY
                     EREAKPADDEMAPPPTVAVAPAGALQKLQLLQQSLKERLADAILYPSTENFVKFFQMQ
                     NYFTQQAGLFERSAQRAFLAHPELDYNLKHSHYNGTVKSQLAADFAAQREAIGKMAQQ
                     YGLMLFYRGAEPIDGQLVSVVKGFRDSYGLSVVPVTIDGVVNPAMPESRRDAGQSQRL
                     GISHFPALVLVDPRSGAVKPLAYGFISQDDLAKQFLNVFTDFKPNY"
     gene            11358..11672
                     /locus_tag="J1138_003619"
     CDS             11358..11672
                     /locus_tag="J1138_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBA8455371.1"
                     /translation="MRKFKFPDIDATGGWVAAFGIWFHIVAGLVHRAPDMAVTLAEFI
                     ALTLTVCGGYKILDELARVPKRQREEAEKQAYIAAHREQAPSPASTDEQHGAGEVQNA
                     DR"
     gene            11659..12195
                     /gene="trbB"
                     /locus_tag="J1138_003620"
     CDS             11659..12195
                     /gene="trbB"
                     /locus_tag="J1138_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBA8455372.1"
                     /translation="MRIVNIAWLALLLTGLAHGSTIDEIAALQKNAGVNRAQSATSVP
                     APRPAAAPRWFSLSNGARVNLNDWKVVLFMQGHCPYCHQFDPLLKSLSERVGFSVFAY
                     TFDGQGDATFPEAIPAPPEVMRTFFPGLPVASPTTFLVNVNTLATYPMIQGASDERGF
                     MARLDTVLQEALMRGGQQ"
     gene            12192..>13512
                     /locus_tag="J1138_003621"
     CDS             12192..>13512
                     /locus_tag="J1138_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBA8455373.1"
                     /translation="MTLRTRFGGIALGLLLTVPAVADMNSDMNQFFDKLGFASNTTAP
                     AVWQGQAAGYISGGSIYARTGVKQIQLISMSLPDINAGCGGIDAYLGAFSFINGEQLQ
                     RFTKQIMSNAAGYFFDLALQTTVPELKDAKDFIQKMASDINSMNMSSCQAAQGIVGGL
                     FPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKGDSVRDRAGEKDKERISKNIN
                     IMWDALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLPPRTTDQSIIKALMEGGA
                     ARVYHCNDSDKCLKVVADSTTTISPDNALKGQIKKMLFSIESKARNDERLTDAEKGLI
                     SSTHVPLLTYLTDPQMLGVSSAMVHQLADYVGYDILMQYLQELMGGARAMLATGNYPE
                     ATVEQINNNFTQAGQLIASLQSRVQVQTDALLVVEQQMRYMRQQVSAR"
BASE COUNT         2780 a         3917 c         4309 g         2506 t
ORIGIN      
        1 cggctttgcc tggggcgccg ggttccttga cgggttgggc gagggtgctg gccgggcggc
       61 gcagccgacc gttggtcttg gggccacggc cagcatgggc gcgggcgata ttgccaaatc
      121 ggccatcggc gggggggcca gcaaagccgc ctcgacgctc agcgactact acatcaagcg
      181 ggcggagcag tatcacccgg tgattccgat cggcgccggt aacgaagtca cggtggtgtt
      241 ccaggagggc ttccagatga aaaccatcga ggaagtggcg gcggaaaaag agagccgtca
      301 gcaggggcag accgaaggga gcgatcagcc caaatccggg caaagcctga acgggttcaa
      361 caccgatcag atgctgaacc acctcggcaa gcttgaaccg gctcagttcg gtctcggcac
      421 atcaacaaca ggagagcggt aacgctatga ataacaacga caaacccaat atctggtatt
      481 tcgatgactt tcccttcatg ggtgtcgctt ctgcagaaat cgggaaccgt gtcgtctgcg
      541 gccgaaccgg tgcggggaaa acgacgctga tgcagtccat cgtggataaa tacagcgcgg
      601 atgccgtgcg gcagggcatt tctattgcac cgaaaccgcc ttctgaggat gaggccaaat
      661 catgactgaa agaaaaatga cggggctata tgtcttttta ttgctggcct ggtggggcgg
      721 ctggcttgca ctcgatatgt ggcgcattga gcacctgctg accgatgccg acaagtgggc
      781 cggttttcgg cgtgacattc tctggtgtgc ggggtcggcg gtggtctccg tgattctttt
      841 cataatgacc gccgacaaaa aactcgccgt ttacgtactg ctgggtttct tggctgcggc
      901 agtgggcatc tcgaccgcgt tcgtcgtcag tgacgctttt attcactggg gatttgatat
      961 gtcgctgttc tcaatggcga tttcggtagt tggaatagcg acggtgccaa tgttgccgtt
     1021 tgcggctttt ctcgtggcaa ggtcataagg agactcaatg aaaacgcaca tcatgaaatc
     1081 actgctttta ctccccgccc tgggtggcgt gctgctgctg gccggttgtg ctggcgtcag
     1141 cagcgaattc ggctgtaacg ccaccacctc tgacagctgc atgacgatgg agcaagctaa
     1201 cgagaaagcc aagtcgctgg aagagggcag cgaagcaaag ccggctgcgg ccgcattgcc
     1261 tcgcctggct gacggcgact ttgcggctgt ggggcgagat atcggccccg ttcctgtatc
     1321 agtcggcagc acttcccgtt tgttgcctcg tcagagggcc gcatcctcgg cgccggtggt
     1381 tccggcgtat ccggcgattg cggcgccgtc cgccatcgtg acgaccgtca gtacctgcac
     1441 gttgccgcgc tgtgccggtg tcggcagcac ccgaccggcc cgccaggccg agcagaccgc
     1501 acagctgtgg atcgcaccct ggattgacag tcaggacgtc tatcaccagc cgggcaaggt
     1561 gctgttcgtg gttaaacccg ccgtctgggg ccagccccag tccgtgtact gacaggagtc
     1621 gtaaatgaat ttgctcgaca aggtcaccca tactgccaac agcctgctgt cggcgctgaa
     1681 aatgccggat gaagccgccg ccgccaacaa ggtgttgggt gacatgaact ttccccagct
     1741 gagcagcgtg ctgccctatc gcgactacga cgaagagagc gggctgttta tcaatgccgg
     1801 ctccatcggc tttgtgctgg aagcgctgcc gctggccggc gccaacgagc aggtggttct
     1861 gacgctcgaa agtctgctgc gcaccaagtt gccgcgtggg atcgcgttgt ccgttcacct
     1921 gatggccagc aagcgcgtcg gcgatctggt ggaatccggt ctgcgtgact tctcctggtc
     1981 gggcaaggac gcagacaaat tcaacgccat cacgcgtgcc tactacctga tggcggcggc
     2041 agaccggttt tccctgccgc cgggcgtcga tatgccgctg accctgcgca actaccgggt
     2101 gttcatctcc tattgtgtca agagcaagcg ccgcaacaaa gcgctcatca ccgagctgga
     2161 gaacctggtc aaggtgctgc gagcgtcgct gatgggggcg aaaattccga cagagtcgct
     2221 taatgacgaa gactttatca acgttgtggg cgaaatcatc aaccacgatc cgagccagct
     2281 gtacaacaaa cgtcgcaaac tcgacccgct caaagacctg aacttccagt gcatcgacaa
     2341 cgggtttggc atggacgtgt atcccgactg cgtcaaaatc aacctgcgtg atgccggcag
     2401 caagaaaggc agcgtggcgc gggtgatgaa tttccagctc gagcagaacc cggagatggc
     2461 cttcctgtgg aacaccgccg acaactacgg caacctgctg taccccgagc tgtcgatcgc
     2521 cacgccgttc attattacgc tgacgctggt cgttgaagaa cagaccaaga cctctttcga
     2581 ggcgaaccgc aagtacatcg atctggagaa aaaatcccgc acctcctacg ccaaattctt
     2641 ccccggcacg ctggaggaga tgaaggaatg gggcgatctg cgccagagtc tggcgtccaa
     2701 ccagacggcg atcgtttcct tcttctataa cgtgacggtg ttctgcgaag acaacgacag
     2761 cgtggcgctg cgttgcgagc aggacgtcat caacagctac cgcaagaacg gctttgattt
     2821 gatatcgccg cgcttcaagc agcactggaa cttcctggcc tgcctgccat ttatggccga
     2881 gcacggcgtc ttccaggatt tggcgatgca tggcgcggtg catcgcgcca agaccctgaa
     2941 cgtggtcaac ctgatgccga tcgtcgccga caaccgtctg gcgccgtccg gtctgctggc
     3001 gcccacctac cgcaaccaac tggcgttcat cgacatctac tccgaggtga tgcgtaacac
     3061 caactacaac atggcggtgg ccgggacgtc aggcgcgggt aaaaccggtc ttattcagcc
     3121 gatgctgcgc agcgtgctgg ataccggtgg cgccgcctgg gtgttcgata tgggcgatgg
     3181 ttacaagagc ctgtgtgaaa acatgggcgg ggtttacatc gacgcccaga cgctgaagtt
     3241 caacccgttc gccaacgtga aggacatcaa cctgtcggcg acgcagatcg ccaacctgat
     3301 ttcggtgatg gccagccccg acggcaacct ggacgaagtt catgagagcc tgctggagat
     3361 ggcggtcaag gcagcctggc tgagcaagca aaagaatgcc cgtatcgatg atgtgcgtga
     3421 ctttctgcgc gacgcaatgg atgaggaggt ctatcgcgat tcgccgacca tccgcaaccg
     3481 cctgcatgaa atgaccatcc tgctggataa atactgctcc gacggcacct atggtgagtt
     3541 cttcaactct gacgaaccgt cgctgcgcga tgacgtcaac atggtggtgc tggagatggg
     3601 cggccttaaa aaccgccctg atctgctggt tgcggtgatg ttctcgctga tgatctacat
     3661 cgagggacgg atgtaccaga catcgcgtca gagcaagaag ctgtgcgtta tcgacgaagg
     3721 ctggaagctg ctcaacttca agaatgccaa ggcgggcgag tttatcgaag agggttaccg
     3781 taccgcgcgc cgtcaccgcg gggcgtacat caccattacc cagaacatca aggactttga
     3841 ctcggacacc gccagctcgg ccgcgaaagc cgcctggggg aactcctcct acaagatcat
     3901 cctgatgcag gacgcctccg agttcaaaac ctatctgcag aaaaacccgg acaacttcac
     3961 ccagctgcag caggacatga tcgggaagtt cgaatcggcc aaagaccagt ggttcagctc
     4021 cttcatgctg atggtctccg gcaaggtcag ctggcaccgc ctgtttgtcg atccgctgag
     4081 ccgcgccatg tactcctccc agggccagga cttcgagttc atgcaggccc ggcgcgaaca
     4141 gggggtatcg gtgcatgacg ccgtgtatga gctggcgcac cgcaactttg gcgaggagat
     4201 ggcgcgcatc gagcgctggg tggaagaaaa ctacacaggg agggcggccg catgacactg
     4261 cctgccatta tcacgccgcc aaatactccg gcacagaaac gcgccattgt gctgtggggt
     4321 acggtctgcg tactgattgc cgttctgctc agcgtggggg ccacgctgtg gctgctgcag
     4381 cgtcagccgg cgatcgtcac cttcgatatg aaacagacca tggaggcgtt ctacgagagc
     4441 gccgccaaac aatccctgcc gccggagcgc agcaagctgc tggccgaacg gtttaccggc
     4501 gcgctgcaaa ctagcatcgc gcaataccag cagcgcaaca acgtgatcat cctggtctcg
     4561 ccggcggtag tgggcggtgc cagggacgtg acacgcgagc tacagcggga tattgccgcc
     4621 aggatgcagc ggggcaatat cgtcagtgta ggtgaggaga caaacctatg aatagttcag
     4681 caatcagttt tatggcgggc tttgttgttt gttttgtaat catgttcgtt acgaatctgg
     4741 ggagaaatca aatgccagcc accatcacgg cgactccagt ggtgtttacc ccggctcagc
     4801 aggatgccat cgggccactt gcccgcgatt acctgctggc gcacccggac atcctgctgc
     4861 agatgagtga gaagctggag gcgcagcaac agactgcggc caatcgcacg gtgcaaaccg
     4921 cgttgaccac gcagctggaa ctgcaggcga cgttactgca cgatccggcg acgccggttg
     4981 tgcatcctga cggcggcgtg accgtggtgc agttcttcga ctatcagtgc atctggtgcg
     5041 cgcgtatggc gccggtggtg gccgactttg tgcgggacaa tcccgatgtg cgctttgtgt
     5101 tcaaggagtt ccccattttt ggcagccgct ggccgatgtc ggtgcaggca gcacgtaccg
     5161 gcctgacgct gtggcaactc aagggcggtg aggcctatct tgcctaccac aatgcggtgt
     5221 ttgcctccgg tgagaacgaa gggcgtttgt cccaacctac gcttaatgcg gccctccaga
     5281 gagccggcgc cgcgccgatg accgtgctgc ccgagacgga gaaaacgttg ctggccacgc
     5341 gcgagctggc ccagcagctg aacatcagcg gcacccccgc ctttattgtt atgccgaccc
     5401 gtgatgcgcg ggcggatcag gtgacgccgc tgtacggcgc cgtcgatgtg aaggcgctga
     5461 aggctgcggt tgcgcaggca aaacaagcgg atattccctc acaggcggtg ccgcatggct
     5521 aaattgacca aagcacaacg cgcggtattg cgcgagaagt ttggcggccg atgcgcctac
     5581 tgcggttgtg aactgccgga gaaaggctgg catgccgatc acctcgagcc ggtgatgcga
     5641 gaatccgagc aggacatggc cgccgccgaa aaaggtctgt tcaaactgaa agcgaccggc
     5701 aaggttttgc atgaaagccg cgatgcaatc gagaacctta acccggcgtg cgccccgtgc
     5761 aacctgttca aaacgacttt cagcctcgag acgttccgcg agcagatagc tgcgcaagct
     5821 gagcgagcgc gggcttacag cgtcaatttc cgcactgccg agcgcttcgg gttgattgaa
     5881 gtcaaacccg cgcccgtcgt gttttggttc gaacggtatc aggaggcaga gcgtggctaa
     5941 gcctttacgt aaaagcctgc tgatagcggt gctcgggctt tcacagctta cgagcatggc
     6001 ggtggccaaa gacttcggca cctggggcga cctgtacccg gtggccgagc cggacatgct
     6061 gcagaccatt cacgaacgtc tgaccgcgct gcagcgcagc ggccgttggg atgaggaaat
     6121 gacggcgttc aaagagcgca cagtgaagca cagccagcgg cctgagccgg tcgccggcat
     6181 cggaaaaacc gagcgctatg ccgaacgctt cttcgacccg agcgtgcagc tttcttctga
     6241 cctgaaagac gacaaggggc gcgtgtttgc ccgcaagggc gacgttatca acccgctggc
     6301 cacagtgccg ttcgcgcaga cgctgtattt catcgacggc gacgatccgc agcaggttgc
     6361 ctggatgaag gcgcagcagc cggagaccct gatggtgaag gtcattctgg tcaacggcga
     6421 cattcccaag acatctgccg cgctcgacag ccgtatctac ttcgatcagg gcggggcgct
     6481 gtcgcgccgg ttcgggctga cgacggtgcc ggcgcgcatc acggcggcgc cgagcggcct
     6541 gcggctgcgt atcgaatcca ttcctgccgc cggcagcgcg aactgaaggg gaccagtatg
     6601 tttgtgacac gtaaacgttt tattgcgacc gagaatcagc tgcagagctg tcggcaactg
     6661 ctggccgcgc gccacggcga gttgatggcc atccgccagg cggcgtcggc aaagggtggg
     6721 atctacaaat gcattctgga gtgccgtgag gtagcccacg agctgcgtga gcagctgcct
     6781 gccgatcagg acggtcactg cccttatttg ttcgacaagc tggcgcttat cgacagtctg
     6841 ctgggcaccc tggctgcgtt attaccgccg caggatgagg cacgtcccac cgcccggatg
     6901 gttgccgccg gtgatgtgca agagatttac cggggaacgc agtgggcgac gcattgcctg
     6961 gggcgtcgcg tatgaccagg tgctggctct cggcgttgct ggtcgtggca ggcctgtttt
     7021 cggcaccgtc tcaggcagcg gatgcggcct gtcaggggcg atttgtcaat ccgatcaccg
     7081 atatctgctg gcgctgcatc ttcccgctct cgattgggag cattccggtc gggaaagggg
     7141 atttgccgga taccagtaat ccgggcagcc ctattcagtt ttgcccggca ccgccgccga
     7201 tttttcaacg tatcgggatg gcgatggggt actgggagcc gatcgccatg actgacgtga
     7261 cccgttcgcc gggctgcatg gtcaatctgg gcagctttag tatcaatctc ggcaagattg
     7321 gcaccggcac gggtggcagc agtggcaaag gggacgtgac caccgcgttc tatcacgtcc
     7381 actggtacaa gtacccgctg acctactggc tgaacatcat cacgtcagtg ggctgcctgc
     7441 agggtggtga cctggatatt gcctatctgt cggaaatcga tccgacctgg gtcgacagca
     7501 gcctgactac cattctcaac ccggaagccg tggtctttgc caacccgctg gcgcagggcg
     7561 cctgcgcggc cgacgccatt gccagcggat tccatctgcc gatcgacgca ttgttctggt
     7621 gtgccggcag tcaggggtcg atgtacccgt tcaacggctg ggtggccaag gaaatcagcc
     7681 cgctgcagtc gtcggtactg gtctcggaaa agatggcatt caagctgcac cgtcaggggc
     7741 aaatcatgga gtccatcggc aaagaccggg cggtctgttt cgagtatccg tcgccgatca
     7801 tgccgaaaga gcggtggcgt tatcagatgg tgaacatgta cccggacagc gctcgctgtc
     7861 acccgatggg gcgcagcgtg atgcgctggg aagtgggcca caactcgccg gccagcaaga
     7921 agaacttcgg ctacgtcatg tggcgcaagc gcaactgcgt ctttttataa ggagaaggtc
     7981 atgacgatat tcagcaaggc gacggggttg ttggcggtgc tcctgacggg ggcaaccgta
     8041 ccgatgatcg ctgtcgggca aatggcggca gatcccgttg ctaccgcaca gcagcaggcg
     8101 accgtccagg tggacagcaa ccgggcatgg ctgcgcgagc aggaaaagcg ctcggctgac
     8161 ctgcaccaaa acaccccggt gccggagttc ctgcgcaatc aaccgccgcg ttcgctggcg
     8221 caggacgacc agtcctttat tgaccagctg gcggcgaagc agcggcagga cgccgtcgag
     8281 caaccggccg agggggcgct gtacttcgtg tccttcgcta ttcccgagga aggtatgcgc
     8341 cgtatgttgc aggaggcgcg ccgcttcggc attccggcca cgttgcgcgg catggtgaat
     8401 ggcgacatgc gcgaaacggt gcagcgggtg caaaaactgg tggaagccgg cggcatcgac
     8461 ggcgtgcaaa tcgacccgca gccctacaca cagtacggcg tgaccgccgt gccagcgttg
     8521 gttgtgcgct gcgcggccgg cttcgatgtg gtgcgcggca acctgctgat ggcgcaggcg
     8581 ctgaagaagg tcgccaagga cggcgactgc gccgccacgg ctcaggcgct gctggacaag
     8641 gagagcggaa aatgacccac ctggtgaagt cactgcccgg actgctggca acacatgccc
     8701 tggtgttggg gacgtttatc ttcgccaccg gcgattgccg ggcggaaggt gatgacgtgt
     8761 tccagaccgg cagcgagttt gccaaacaga tcgccggtca gggcacgggg acgctgcaga
     8821 acaccaaccc ggccaacgtg ctgccgaact acaccgacaa cccgaaagaa aaaggctatt
     8881 acggcggcgt gacggccggg gatgccaaca atctcaagac agacggcggc accgcatggg
     8941 gccagaccga ggtggggcag gcggtgaccg attcggtgct caagaacccc aaggagccga
     9001 tttctcatga tgcgcccttt atccaggccg gcaaggagat tgaaggcaag gccgagagca
     9061 tcgtcggcaa gaccggcccg gactgcaagg ccgagcaggt gacgcgcagt gaattcaccc
     9121 gccatgtgtg cgagcgcgat acggcggtga tgcgtacctg cgagcgcgcc accgacatcg
     9181 gtggccacta tgagacccgc tatgaagacc gtaacgcaca catcgataaa tcgatgatcc
     9241 gttggtggaa agaaggcaac cgcattcacg gcacctttac ggcgccgttt accggcaagc
     9301 tcactacggg ttcgatatac ctcgagctgc aggacgatct gcacagctat ggcgtaccac
     9361 tggccttttc actgctgggg aatgaggtgc gctatggccg tcaggactgg ggcaaccggc
     9421 cgctgaacgt cggtaacgtc acgctcacgc aaggtcagca ggtcgatatt ttcctcacca
     9481 tggaccccgg tggccacctg gatacctttc tggatgcgat ggtcaggcag ctgagcggtg
     9541 gtgggggcgg ctcgccgatg gtgttctacg tgaagttcga cggcagttcg tcggtgcagg
     9601 tctgggtgcc gaccacgtcg acgccggcag actgtccact ggcgcaggag gccggggcgg
     9661 ttaaggtcaa taccgtctgc agtgagccag gcggggaacg cacggtgacg gtcaacggcc
     9721 agacctaccc ggtgtgggcc agctgctggc gctttaccga cacctatcgg ctgcaggagg
     9781 ccggccaggg cagctgcgaa gcctatgcca acaatccggc ctgcactatt gccacgcacg
     9841 gctgtgcctt ctccgatgag tatggccagt gcctgcacga gaacgtgacc tactcctgcg
     9901 aaacccgcac caccgggaac gtgatgctgt gcggcggcca gatgtactgc ctggacgggg
     9961 agtgcgacaa ggcgcagaag ggcacccagg gtaacgattt tgcgcacgcc gtttccgagc
    10021 tggccgcagt ggcggccgcc ggcaaggacg tggcggccct gaacggcatt gatgtgcgcg
    10081 ccttcaccgg taaggggcag tcctgccgca aggcgtttgc cggtttcagc gactgctgca
    10141 aggactccgg gtggggtaac agcgtcgggc tggcgcactg cgacagcgaa gagaaggcgc
    10201 tgggcaaggc gaaagagaac aaattgaccg tcagcgtcgg cgaatactgc agcaagaaag
    10261 tgctgggtgt ctgcctgcag aagaagcgca gctactgcca gttcgacagc aagctggcgc
    10321 agattgttca gcagcagggg cgcgcgtggc agctgggtat cggctttggc gacgcaaaat
    10381 cccccgactg ccggggcatt accgtagacg agctgcagcg catcaacttc gaccagctgg
    10441 acttcgccaa tttctatgaa gacctgatga agaaccagaa ggtgcccgaa gacgcggcac
    10501 tgctggagaa ggttaagcag caaatcgcac aacgtatgca ggaggcagcc aagtgaaaac
    10561 gcgcgggact tcaaatgtca atttggcggc acgcgggttg gccaaccgca gggcggcagc
    10621 gctgttgggc ctgagcctgc tgggcgcggc gttcaccgcg caggccggcg gctggcagtg
    10681 gtatgagcgg gaggccaaac ccgctgacga cgagatggca ccgccgccga cagtagcggt
    10741 cgcgccggcc ggcgcactgc aaaagttgca gctgctgcag cagtcgctca aggaacggct
    10801 ggcagacgcc atcctgtacc ccagcaccga gaacttcgtg aagttcttcc agatgcagaa
    10861 ctactttacc cagcaggcgg ggctgtttga gcgctctgcg cagcgcgcgt tcctggcgca
    10921 cccggagctg gactacaacc tgaagcacag ccactacaac ggcacggtga aaagccagct
    10981 ggccgccgac tttgccgcgc agcgcgaggc catcggcaaa atggcgcagc agtacgggct
    11041 gatgctgttc tatcgcggcg cggagccgat cgacggtcag ctggtgagcg tggtgaaggg
    11101 ctttcgggac agctacgggc tgtcggtggt gccggtgact atcgacgggg tggtcaaccc
    11161 tgcgatgccg gagagtcggc gcgatgcggg acagtcacaa cgactgggca tcagccactt
    11221 cccggcgctg gtgctggttg acccgcgcag cggcgcggtc aagccgctgg cctacggctt
    11281 tatcagccag gacgatctgg ccaaacagtt cctgaacgtc tttaccgact tcaaacccaa
    11341 ttattaagga gcgttgaatg cgtaaattta aattccctga catcgacgcg accggcggtt
    11401 gggtcgcggc cttcgggatc tggttccata ttgtggccgg cctggtgcac cgggcgccgg
    11461 acatggcggt gacgctggcc gagtttatcg cgctcaccct gacggtgtgc ggcggctaca
    11521 aaatcctcga tgagctggcg cgcgtcccca aacggcagcg tgaggaggcg gaaaagcagg
    11581 cctatatcgc ggcacatcgt gaacaggcgc cgtcgccggc aagcacagac gaacagcacg
    11641 gggccgggga ggtgcagaat gcggatcgtt aacatcgcct ggctggcgct gctgttaacc
    11701 gggctggcac atggctccac gattgatgag atagcggcgc tgcagaaaaa cgccggcgtc
    11761 aatcgggcgc aatcagcgac gtccgtcccc gctccgcggc cggctgcagc gccacgctgg
    11821 ttttcgctca gcaacggcgc gcgcgtcaac ctcaatgact ggaaggtggt gctgtttatg
    11881 caggggcatt gcccgtactg ccaccagttt gatccgctgc tgaaatcgct gtcggagcgg
    11941 gtcggctttt ccgtgttcgc ctacaccttc gacggccagg gcgatgctac gttcccggag
    12001 gccatcccgg cgccgccgga ggtgatgcgc accttcttcc ccggcctgcc ggtggcgtcg
    12061 ccgacaacgt ttttggtgaa cgtcaatacg ctggcgacct acccgatgat ccagggggcc
    12121 agcgatgagc gcggctttat ggcgcgtctt gataccgtgt tacaggaagc gctgatgagg
    12181 ggaggtcaac aatgactttg cgcactcggt ttggcggcat cgcactgggg ctgcttttga
    12241 cggtgccggc cgtggccgac atgaacagcg acatgaacca gttttttgac aagctggggt
    12301 ttgcgtccaa caccacggca ccggcggtct ggcaggggca ggcggccggg tatatctcgg
    12361 gcgggtcgat ttacgcccgc accggcgtga agcaaataca gctgatctcg atgtcgttgc
    12421 cggatatcaa tgccggttgc ggcggcatcg atgcgtacct gggcgcgttc agctttatca
    12481 acggcgagca gctgcagcgc tttaccaaac agatcatgtc caacgccgcc ggctacttct
    12541 tcgatctggc cttgcagacc accgtgccgg agctgaaaga cgccaaagac ttcatccaga
    12601 aaatggccag cgacatcaac agcatgaaca tgagcagctg ccaggcggcg cagggcattg
    12661 tcggcgggct gttcccgcgc acgcaggtct ctcagcagaa ggtctgccag gacattgccg
    12721 gcgaatccaa catcttcgcc gactgggctg cctcgcggca gggctgcacg gtgggtggca
    12781 aaggggactc ggtgcgtgac cgggcaggag agaaggataa ggagcgtatc tccaagaaca
    12841 tcaacatcat gtgggatgcg ttatccaaaa accggatgtt cgacggcaac aaggagttga
    12901 aggagttcgt gatgacgctg accggctcgc tggtgttcgg ccccaacggc gaaatcacgc
    12961 cgctgccgcc acgcactacc gatcagagca ttatcaaggc gctgatggaa ggcggcgcgg
    13021 cccgcgttta tcactgcaac gacagcgaca agtgcctgaa ggtggtggcc gacagcacca
    13081 cgacgataag tccggacaat gccctgaaag ggcaaatcaa aaaaatgctg ttcagcatcg
    13141 agagcaaggc gcgcaacgac gagcggctga cggatgcgga gaaaggtctg atttccagca
    13201 cgcatgtacc gttgctgact tacctgaccg acccgcaaat gctcggcgtg tccagcgcga
    13261 tggtgcacca actggccgac tacgtgggtt acgacatcct gatgcagtac ctgcaggagc
    13321 tgatgggcgg cgcgcgcgcc atgctggcga ccgggaacta cccggaggcc accgttgagc
    13381 aaatcaacaa caacttcacg caggccgggc agcttatcgc ctccctgcaa tcgcgcgttc
    13441 aggtgcagac cgatgccctg ctggtcgtcg agcagcagat gcgttacatg cgccagcaag
    13501 tgtcggcacg cc
//
LOCUS       DADTFG010000132        13504 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.133, whole genome shotgun sequence.
ACCESSION   DADTFG010000132 DADTFG010000000
VERSION     DADTFG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.133"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(107..859)
                     /gene="yecC"
                     /locus_tag="J1138_003622"
     CDS             complement(107..859)
                     /gene="yecC"
                     /locus_tag="J1138_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBA8455374.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(856..1524)
                     /gene="tcyL"
                     /locus_tag="J1138_003623"
     CDS             complement(856..1524)
                     /gene="tcyL"
                     /locus_tag="J1138_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBA8455375.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1539..2525)
                     /gene="dcyD"
                     /locus_tag="J1138_003624"
     CDS             complement(1539..2525)
                     /gene="dcyD"
                     /locus_tag="J1138_003624"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBA8455376.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNTQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2630..3430)
                     /gene="tcyJ"
                     /locus_tag="J1138_003625"
     CDS             complement(2630..3430)
                     /gene="tcyJ"
                     /locus_tag="J1138_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8455377.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3518..4069)
                     /gene="fliZ"
                     /locus_tag="J1138_003626"
     CDS             complement(3518..4069)
                     /gene="fliZ"
                     /locus_tag="J1138_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBA8455378.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(4115..4834)
                     /locus_tag="J1138_003627"
     CDS             complement(4115..4834)
                     /locus_tag="J1138_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBA8455379.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5154..6464)
                     /locus_tag="J1138_003628"
     CDS             complement(5154..6464)
                     /locus_tag="J1138_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HBA8455380.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTISIDL
                     QKIDSSTLGLNGFSVSGQSLNVSDSITQITGAAGTKPVGVDFTAVAKDLTTATGKTVD
                     VSSLTLHNTLDAKGAATSQFVVQSGNDFYSASINHTDGKVTLNKADVEYTDTDNGLTT
                     AATQKDQLIKVAADSDGSAAGYVTFQGKNYATTVSTALDDNTAAKATDNKVVVELSTA
                     KPTAQFSGASSADPLALLDKAIAQVDTFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQS
                     RIQDADYATEVSNMSKAQIIQQAGNSVLSKANQVPQQVLSLLQG"
     gene            6712..8127
                     /gene="fliD"
                     /locus_tag="J1138_003629"
     CDS             6712..8127
                     /gene="fliD"
                     /locus_tag="J1138_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBA8455381.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSDNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            8143..8553
                     /gene="fliS"
                     /locus_tag="J1138_003630"
     CDS             8143..8553
                     /gene="fliS"
                     /locus_tag="J1138_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBA8455382.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            8553..8918
                     /gene="fliT"
                     /locus_tag="J1138_003631"
     CDS             8553..8918
                     /gene="fliT"
                     /locus_tag="J1138_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBA8455383.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8996..10483
                     /gene="amyA"
                     /locus_tag="J1138_003632"
     CDS             8996..10483
                     /gene="amyA"
                     /locus_tag="J1138_003632"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBA8455384.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10517..10930)
                     /locus_tag="J1138_003633"
     CDS             complement(10517..10930)
                     /locus_tag="J1138_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA8455385.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11117..12322
                     /gene="yedE"
                     /locus_tag="J1138_003634"
     CDS             11117..12322
                     /gene="yedE"
                     /locus_tag="J1138_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBA8455386.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            12319..12552
                     /gene="yedF"
                     /locus_tag="J1138_003635"
     CDS             12319..12552
                     /gene="yedF"
                     /locus_tag="J1138_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBA8455387.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12661..13329
                     /locus_tag="J1138_003636"
     CDS             12661..13329
                     /locus_tag="J1138_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBA8455388.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFIAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPRVLSPEAAREWMRQEVGDKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAEL
                     IQPV"
BASE COUNT         3332 a         3477 c         3366 g         3329 t
ORIGIN      
        1 ttctcttatg ttattaatga gttatgctga tttgttaagc agttttatca cgtttgaaat
       61 ggcgtcccgc cccgactggt gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa
      121 acttctccag gaactggcgg gtgcgaggct gctgaggatc agcaaataac gcttttgcta
      181 ccccctgctc gactatccgc ccctggtcca taaaaatcgc ccggtccgca acatcccggg
      241 caaagctcat ttcgtgcgtc acaatcacca tcgtgcgctt ttcctgcgcc agctgacgaa
      301 tggtgttcag gacttcgccc accagctctg gatccagcgc cgacgttggc tcatcaaaca
      361 aaatcacttc aggacgcatt gccagagcac gcgcaatcgc aacgcgctgc tgttgaccgc
      421 cagacaaacg acgtggataa ctggtttctt taccggccag cccaactttc gccagcagct
      481 cgcgagcgcg cgccgtggcc tcttctttcg gttcgccttt gacgatcacc ggcccttcaa
      541 taatgttttc cagcaccgta cgatgcggaa acagattaaa gttctggaag acaaacccga
      601 cgtgctgacg caactggcga atcagcgatt tttgctgact taatgaacgt gcagtatcaa
      661 tagtgatatc gccaacggtg atggttcccg cttccggttg ttccagcaga tttatgctgc
      721 gtagcaacgt ggttttgccg gaaccactcg gaccgataat agccactact tcgccaggct
      781 tcacctcaag gtcgatacca tgcagcaccg tctgaccgtg gaattttttc accaggttct
      841 taacttcaat ggcactcatt tgggttccct ctcctggcga ttaagttgat tctcaaaatg
      901 gttctgcaac gtcgataaca cagtcgccat aatccagtag atgagcgaag ccgccaggta
      961 catggtgaac acctccagcg tgcgcgaggt aatcaactga gcctgacgga acagctccgg
     1021 cacctggatt gtcgcggcca gcgacgtatc ttttaccagg ctgataaagc tgttcgacag
     1081 cggcggcaac gccacccgcg ccgcctgtgg caaaatcgcg cgacgcatgg tctgccatgg
     1141 cgtcatgcca atactggctg cagcttccca ctgaccttta tcaatagaag aaatggcggc
     1201 gcgcagcgtt tcggcagcat aggcggcagt gttcagcgac agaccaatca ttgctgacgg
     1261 aatgggatct aattcgatac caaactgtgg cagtccgtaa tagatcataa acagttgtgc
     1321 gatgagcggc gtaccacgaa aaatagagat ataaaaacgc gccagccagc gcacgggcca
     1381 gattggcgac aggcgcatca gcgcgagaat aaacccgagc agtaagccaa aaaacatgcc
     1441 gccgatactg agttgcagcg tatacccagc ccctttcagc aggaacggca aggaatcaat
     1501 aaccagttgt atactttctt gcatgagcgt tcccggagct aaacgtgggg atgataggcg
     1561 aacagcgcag gcgcaccgcc ggtatgaata aacaaaatcg gcccttcatc tttgaagcgt
     1621 ttctgactga taccgtcaat cagccccgcc atcgcttttc cggtatacac aggatcaagc
     1681 agaatgcctt ccagccgcgc cagcaatttc actgcttcca tgccttcgtc gttcggcacg
     1741 ccgtagccag gtgcaaaata gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc
     1801 agttctttcg caatcgcctg ttggaggtta accactttcg gcaattgatc ggcaacagaa
     1861 cgcgacacag tcacgccaat cagttcgctt tcaggcatca ggtgttccag cccaacagcc
     1921 agtccggcgt gagttccggc actgcccgat gcgaccacca ccgacgaaat attaaccgcc
     1981 ccttcacact gttgcgcgat ttccagcgca ctctccacat aacccagcgc gcccaaagca
     2041 ttagaaccgc caaccggaat gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc
     2101 agctcttcca gttgggtatt gggatcggtc agtgcgtcgc acatttcaat ctgggtattg
     2161 aacagatcca gcaacaaacg attgccgttg gttaaatagt tttctgcggt tgtgccaata
     2221 ggattttcca gcagcgccac gcagtgcaga ccgagtttcg cagcgactgc ggcagtctgg
     2281 cgcacatggt tagactggat cgccccggca gtaatcagcg tatcggcacc ttcacgcaga
     2341 gcatctgccg cgagaaattc cagcttacgt aatttattgc cgcccattgc catgggagtg
     2401 acgtcatccc gtttgatgaa aatttcccgt ccaagataat cagaaaagcg cggcagatat
     2461 tcgagcggcg ttggcgcgcc gataaactcc agccgtggaa aacgggttaa attatgcagt
     2521 ggcataacag cctccgatgt gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg
     2581 taaataaaaa aggcgctagt gaaagcgccc ttttttgtca ttatgctgat tatttggtca
     2641 catcagcacc aaaccatttt tcggaaaggg cttgcaaagt gccatctttt tgcatttccg
     2701 caattgcatc attcaccgct ttcagcaggt cttcatttcc tttacgcagc gccacgccag
     2761 actcctgacg ggagaatgct tcaccggtta ctgccagcgt atcgttggtt tttttcacca
     2821 gatccagcgc cgccagacga tcaacgagga tcgcatcgat acggcctacg cgcagatcct
     2881 gatatttggt cgggtcatca tcataggtac gcacatcgac gccctgaaca ttctgacgca
     2941 gccactcttc atagttggtg cccagaccga cacccacttt tttgcctttc agatcatcag
     3001 ccgttttaat ggtgccttcg ttgccttttt tcaccagcgc ctgaatacca gaaatggtgt
     3061 acggggtaga gaaatcgtat tttttcttgc gctcgtcaga aatggtgacc tggttaatca
     3121 ccacatcaat gcgtttagag tccagcgacg ccagcatacc gtcccatttg gtcggtttta
     3181 gtgacgcctc aacgccaaga tgttttgcca gctgttgggc aaattccact tcaaaaccgg
     3241 ttaatttgcc gtcatccccc tgaaaactga acggcggata agttccttcc agccctacca
     3301 gcagcgtgcc gcgctctttg actttattaa gcagaccttc atctgcaaaa cttttaacgc
     3361 tcatacccgc aaccagcgcc acggccatca cacccatcaa tgcctgacgt cccagatgtg
     3421 ctaatttcat atttaccccg aatgttgtta tgtctgtttg cagtgtagag ccattgtttg
     3481 taaacacaaa aacaactccg ctacatctta ttcttattta atatatatca gaagcaggtt
     3541 ggctggagga tttctgcaca agtccggtac acgtttgacg ctgatagtgt tgatacttcc
     3601 gtaacgcgat gcggtaattg ttggtgctgg tggagggcag ccacggggcg aggatttcat
     3661 caaggaaacc gtcctgcagc tgatcgaggg aaatattttg ctcgtgtaga tgattcccca
     3721 aacggcgcag acgaacgaca tattcacgca ccgtaccagg gctcatttca gtttgctcaa
     3781 aaagaaattg cttaaagccg ataatatcga aaaaatcact ctgcgttttg caatgtaaat
     3841 caccgcaaaa tcggcacaat gccgcccatg attgttgttc cacttgccag ccattttcat
     3901 caagcagcgt gtccaggcgg gaaatctcga ttttattcac tattttgccg tcgcgaacga
     3961 gggtaatgcg atcgagtaat ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt
     4021 ctttaagata gcggcttaat ggccgtcttt tcaggtgctg caccatcatt aagaactcct
     4081 ggtagtcaaa attaaagtgc ggcatttact gacgttataa cttacccagt ttagtgcgta
     4141 accgtttaat agcctggctg tgtaactgac tgacccgcga ttccccgacc tccagcaccg
     4201 caccaatctc tttgagattc agctcttcct gataatagag ggttaatacc agtttttcgc
     4261 gctccggcaa cgtttcgatg gcttccatca cccgctggcg cagattactg tccagcagtt
     4321 gttgtagcgg gttttctcgc tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct
     4381 cttcgcgcca ctcatcgtag gagaagagct ggctgttatt ggtgtcgagc aacatttggc
     4441 gataatcggc aatatcgatc cctaaacgtt ccgcaacctc agtttccgtg gcgttgcggc
     4501 caagttcctg ctccagttgc cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc
     4561 tgcgcggcac ccagtcacgg ctgcgaagtt catccagcat agcgccacgg atacgctgca
     4621 ctgcgtaagt tgtaaatgcc gttccttgta gggcgtcata gcgttcgacg gcattaagta
     4681 acccaatgcc gcccgcctgt agcagatcgt caagttccac gctcgcgggc agtcgaacct
     4741 gcaggcgcaa tgcttcgtga cgcaccagcg ggacataacg ctgccacagc gagtgtttat
     4801 ccattacacc ttcagcggta tagagtgaat tcacgataaa cagccctgcg ttatatgagt
     4861 tatcggcatg attatccgtt tctgcagggt ttttaatcgg acgattagtg ggtgaaatga
     4921 ggggttattt gggggttacc ggtaaattgc gggcagaaaa aaccccgccg ttggcgggga
     4981 agcacgttgc tgacaaatta ccattcatgt tgccggatgc ggcgtaaacg ccttatccgg
     5041 cctacaaaaa tgtgcaaatt caataaattg caattcccct tgtaggcctg ataagcgcag
     5101 cgcatcaggc aatttggcgt tgccgtcagt ctcagttaat caggttacaa cgattaaccc
     5161 tgcagcagag acagaacctg ctgcggaacc tggttagctt tggacagcac cgagttacct
     5221 gcctgctgga tgatctgcgc tttcgacatg ttggacactt ctgtagcata gtcggcgtcc
     5281 tgaatacggg actgcgcttc agacaggttg gtggtggtgt tgttcaggtt ggttactgcg
     5341 gaatccagac ggttttgcac cgcaccgagg gaggagcgga aagtatcaac ctgtgcaata
     5401 gctttgtcta aaagtgccag tggatcagca gaagaagccc ctgagaactg tgcagtcggt
     5461 tttgctgttg ataattcaac aacaacttta ttatctgttg cttttgccgc agtattatca
     5521 tcaagtgccg ttgaaaccgt tgtagcgtag tttttacctt ggaatgttac atatcccgca
     5581 gccgagccgt cagagtcagc ggcaacttta atcagttgat ctttctgagt agccgcagtc
     5641 gttagtccat tatcggtgtc tgtgtattcg acatcggctt tattcaacgt gactttgccg
     5701 tctgtatgat taatcgacgc ggagtagaaa tcattgccgg attgaacgac gaactgtgat
     5761 gtagcagccc ctttcgcatc cagagtgttg tgtaacgtca ggctagaaac atcgactgtt
     5821 ttacctgtcg cagtagtcag atctttcgca acagcagtga aatcaacacc aacaggtttt
     5881 gtcccggcgg caccggtaat ttgagtaatg gaatcactaa cgttaagtga ctgacccgaa
     5941 acggagaaac cgttcagtcc taatgtagaa gagtcaatct tctgcaaatc aatgctaata
     6001 gtctgcccat cattcgcgcc aacctgaatc ttcatcgaac catctttcgc cagcacattc
     6061 acgccgttga actgggtctg accggaaacg cgatcaattt catccagacg ggatttgatt
     6121 tcgtcctgaa tagaagacag gtcagaatca gagttagtcc cggtcgtcgc ctgaacggtc
     6181 aactcacgca cacgctgcaa gttgttgttg atttcagaca gcgcgccttc agtggtctgc
     6241 gccagagaga taccgtcgtt ggcgttacgt gcggcctgag tcagaccttt gatgttagaa
     6301 gtgaagcggt tagcaatcgc ctggcccgcg gcgtcatctt tagcgctgtt aatacgcaga
     6361 ccagaagaga ggcgctcgat agaagtcgac agcgcagact ggtttttgtt gatgttgttc
     6421 tgagtgatca gcgagaggct gttggtatta atgacttgtg ccataattca ttttcctgtt
     6481 ttcaagtcta cggtttaagt attgggcttc cacccttcgg cttcgtcgcc gtcaaccctg
     6541 ttatcgtctg gtcactcaca acctttagaa tttttttcta gcggtcgaaa tactcccctt
     6601 cctcgttgct gttcgtgcca ttaaggcaaa aaataaatcg taaactttac cccgcttacg
     6661 ccgataaccc cggtattcgt tctatacacg tcgcatgatt aaggaaacta tatggctagt
     6721 atttcaacgc ttggggttgg gtcggggctg gacttaagca gcatccttga cagtctggag
     6781 gcggcagaaa aatctaccct gacgccgata tcaaaacagc aaagctctta caccgcgaaa
     6841 ctgagcgcct acgggacgct gaaaagtgca ctagagagtt tccagacagc caacacagca
     6901 ctgaataaag ccgatttatt taccgccacc tcaaccacca gcagttcatc ggctttcagc
     6961 gccaccacca cgggcagcgc gattgctggg aaatacacca ttagcgttag ccaactggcg
     7021 caggcacaga cgctgacaac aaaaaatagt caaaaagaca gtaaagccgc catcgccacc
     7081 agcgatagcg tactgaccat ccaacaaggc ggcggtaaag atccggtcac gattgatatc
     7141 agcgcgggta actcgtcact ttccggcatt cgcgatgcca ttaacaacgc caaagctggt
     7201 gtcagcgcca gtatcatcaa tgtgggaaac ggcgaatatc gtctgtccat caccgctaac
     7261 gatacaggca gcgataacgc tatgaaactt agcgtcagcg gcgacagcgc cctggaaagt
     7321 tttatgggtt acaacggaac gccgggtgat agcagcaatg gcatgattga aagcgtcacc
     7381 gcgcaaaacg ccaaactgac agtcaataac gttgagattg aaaacagcag taacactatc
     7441 agcgactcac tggaagatat caccctcaac ctgaatgacg tcaccaccgg taatcagaca
     7501 ctgaccatca gtaaagacac atccaaagct gaaaatgcgg ttaaagcgtg ggtggatgcc
     7561 tataacacat tgcaggatac attcagcagc ttaaccaaat acaccgctgt cgatgccgga
     7621 gctgaaagtc aggactccag taatggcgca ttactcggtg actccacact gcgtaccatc
     7681 cagactcagc ttaaaacctt gcttgccaat actcacagca gttcaaacta taaaacgcta
     7741 gcacagattg gcattaccag cgatgccagt accggcaagc tggaaatcgc gactgacaaa
     7801 ttgcaaacgg cgctaaaaaa tgatgccgct ggtatcggtg aaatgtttat aggtgacggc
     7861 aaaagtactg gggtaaccac cggcattagt agcaacctga ccagttggct ctcttccacc
     7921 gggataatcc aggcagcaaa agatggcgtc agcaaaacgc tgaataattt gaccgaccag
     7981 tacaacgcag ccagtgaacg tatcgatacc ttaatgacgc gctataaagc ccagttcacc
     8041 caattagatg tgttaatgaa ctcgctgaac tccactagta gctatctgac acagcagttc
     8101 gacacctcaa atagcaactc gaaataatct tcaggagaga ctatgtacgc ggcaaaaggc
     8161 acccaggcct atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag ccagcagcag
     8221 ctggtcacca tgctatttga tggagtgctg agcgcactgg ttagagcgcg cctgtttatg
     8281 caggacaaca atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa catcattgag
     8341 aacgggctgc gggtgagtct tgatgaagag agcaaagacg aactaaccca aaacttgatt
     8401 gctctttata gctatatggt caggcgcttg ctgcaagcca atttacgcaa cgatgtctcc
     8461 gcagtcgaag aagtggaagc attaatgcgc aatattgccg atgcctggaa agagtcttta
     8521 ctctcccctt ctttgattca ggacccagac tgatgaacaa tgcaccgcat ttgtatttcg
     8581 cctggcaaca actcgtcgaa aaaagtcagc tcatgttacg cctggcgaca gaagaacaat
     8641 gggacgaact catcgccagc gaaatggcgt atgtgaatgc ggtgcaggag atcgcacacc
     8701 tgactgaaga ggttgccccg tccaccacga tgcaggagca gctccgcccg atgctgcgcc
     8761 tgattctcga caacgaaagc aaggtaaagc agttattaca gattcggatg gatgaactgg
     8821 cgaaactggt cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg
     8881 gcggctttgt gctggctccg caggataacc tcttttgaat cttaatgagc cgatggctcg
     8941 cgaataatcc gattacggct acgcttctaa tattcccctt gaatggagtc gaagaatgcg
     9001 taatcccacg ctgttacaat gttttcactg gtattacccg gaaggcggta agctctggcc
     9061 tgaactggcc gagcgcgcag acggttttaa tgatattggt atcaatatgg tctggttgcc
     9121 gcccgcctat aaaggcgcat cgggcgggta ttcggtcggc tacgactcct atgatttatt
     9181 tgatttaggt gagtttgatc agaaaggcag catccctact aaatatggtg ataaagcaca
     9241 actgctggcc gccattgacg ccctgaaacg taatgacatt gcggtgctgc tggacgtggt
     9301 agtcaaccac aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc
     9361 tgatgaccgt acgcaaattg acgaagaaat cattgagtgt gaaggctgga cgcgttacac
     9421 cttccccgct cgtgccgggc aatactcgca gtttatctgg gatttcaaat gttttagcgg
     9481 tatcgaccat atcgaaaacc ctgatgaaga tggcattttt aaaattgtta acgactatac
     9541 cggagaaggc tggaacgatc aggttgatga tgaactcggt aatttcgatt atctgatggg
     9601 tgagaatatc gattttcgca atcatgccgt gacggaagag attaaatact gggcgcgctg
     9661 ggtgatggaa caaacgcaat gcgacggctt tcgtcttgat gcggtcaaac atattccggc
     9721 atggttttat aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat
     9781 tgtggctgaa tactggtcgc atgaagtcga taaactgcaa acgtatattg atcaggtgga
     9841 aggcaaaacc atgctgtttg atgcgccgct gcagatgaaa ttccatgaag catcgcgcat
     9901 gggtcgcgac tacgacatga cgcagatctt cactgggacg ctggtggaag ccgatccttt
     9961 tcacgccgta accctcgttg ccaaccacga cacccaaccg ttgcaagccc tcgaagcacc
    10021 ggtcgaaccg tggtttaaac cgctggcgta tgccttaatt ttgttgcggg aaaatggcgt
    10081 tccttcggtg ttctatccgg acctctacgg tgcacattac gaagatgtcg gtggtgacgg
    10141 gcaaacctat ccgatagata tgccaataat cgaacagctt gatgagttaa ttctcgcccg
    10201 tcagcgtttc gcccacggtg tacagacgtt atttttcgac catccgaact gcattgcctt
    10261 tagccgcagt ggcaccgacg aatatcccgg ctgcgtggtg gtcatgtcga acggagatga
    10321 tggcgaaaaa accattcatc tgggagagaa ttacggcaat aaaacctggc gtgattttct
    10381 ggggaaccgg caagagagcg tagtgacaga cgaaaacggc gaagcgacct tcttttgcaa
    10441 cggcggcagc gttagcgtgt gggttatcga agaggtgatt taaatccatc cccggcggca
    10501 agccggggag atttcattac ggcagtggcg tcggtaacgg cgctttatcc agcgcggcgg
    10561 cacactctgc cgtcgggcga tccacacgct ccatcgtcat accatcgtac tcaatggtgt
    10621 taccgtcacg ctcaacctcg tacagctcac gttttacggt aacgttagtg agatcatcag
    10681 acatcaatgt caacttaccc ggcacggcaa tcacccgctg ccactgacgg caatccaggg
    10741 tatcgccctc tttagtgaca atgaggctgc ctatcgcttc cggactgacc agcgcacgtt
    10801 gcggcccttt ggtctgccag taacccgtca gccagtccgg cgcgggggtt ttcacgacat
    10861 tgttatagtt ttcgacttcg gcacagcccg ccagcaacat gagcgcacct gcaattgcga
    10921 gttttttcat cattcgtcct gcatgagaag aaaagaggat tgtggcatta aagccctgat
    10981 gtcgccagcc tttccaggct cagagagaaa tttgatctaa ccattatttt tgtgaattat
    11041 taccccgaaa ataaatttgc atggtgtatg attcgcgact cttgtttcaa accttctgag
    11101 ttcagaggct acattcatgt catggcagca attcaaacac gcctggttga ttaaattctg
    11161 ggcacccatc cctgcggtca tcgcggcggg tattctctcc acttactatt ttggcatcac
    11221 tggcaccttt tgggcagtca cgggtgaatt tacccgttgg ggcggtcagc tcctgcagtt
    11281 gttcggcgtc catgctgaag agtggggtta ttttaaaatt atccatctgg aaggatcgcc
    11341 attaacccgc atcgacggaa tgatgatcct cggtatgttt ggcggctgtt ttgccgcagc
    11401 gctgtgggcc aacaatgtca aactgcgcat gccgcgcagc cgtatccgca ttatgcaggc
    11461 cattattggc ggtattatcg ccggttttgg cgcacgtctg gcaatgggct gtaacctggc
    11521 ggcgttcttt accggaattc cacagttctc gctgcacgcc tggttctttg ccatcgccac
    11581 tgccattggt tcatggtttg gcgcgcgctt taccctgctg cccatcttcc gtattcccgt
    11641 gaaaatgcag aaagtttctg ctgcctcacc gctgacgcaa aaaccggatc aggcgcggcg
    11701 tcgttttcgt ctcgggatgc tggtcttttt cggcatgctg ggctgggcgc tgctgacggc
    11761 gatgaaccag cccaaactgg ggctggcaat gctgtttggc gtcggctttg gtttactgat
    11821 tgaacgtgcg caaatctgct ttacttcggc gttccgcgat atgtggatca ccggacgtac
    11881 ccatatggcg aaagcaatca ttatcggtat ggcggtaagt gccatcggga tcttcagtta
    11941 cgtacagtta ggcgttgaac ccaaaatcat gtgggcagga ccaaacgcgg taattggtgg
    12001 tttactgttt ggttttggca tcgtgctggc aggcggctgc gaaaccggct ggatgtaccg
    12061 cgcggtagaa ggccaggtgc actactggtg ggtcggtctg ggcaacgtga tcggctcaac
    12121 gattttggcg tactactggg atgatttcgc tccggcgctg gccaccgact gggacaaaat
    12181 caacctcctg aaaacctttg gcccgatggg cggcctgctg gtgacatatt tgctgttgtt
    12241 tgcagctcta atgttgatta tcggctggga aaaacgcttc ttccgccgtg cggcaccgca
    12301 gattgctaag gagatcgcat gaaaaatatc gttcctgatt accgtcttga tatggtgggt
    12361 gaaccctgcc cttatccggc tgtcgcaacc cttgaggcga tgccgcagtt aaaaaaaggg
    12421 gagatcctgg aagtggtgag cgactgtccg cagtcgatca ataatattcc actggatgcg
    12481 cgtaatcacg gctatacggt gctggatatt cagcaagacg ggccgaccat tcgttattta
    12541 attcagaagt aatccctcat tcccgctgtg gcccacagcg ggaacatcgt tacctctcta
    12601 tcacatagat tttacccgcc ccctctccta ttcttttcag tgtgacctca ggaggtttct
    12661 atgtgcggac gctttgccca atcccaaacg cgtgaagatt acctcgcgtt tctcgcggaa
    12721 gatattgaac gcgatattcc ctacgatccc gaacccattg gcagatacaa cgtcgcgccg
    12781 ggaaccaaag ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc
    12841 tggggatatg ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact
    12901 gccgcaacca gtcgtatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc
    12961 gatggctggt ttgagtggaa aaaagaaggc gacaaaaaac agccttattt tatctatcgc
    13021 gctgatggac aacctgtttt tatagccgcg ataggtagca caccgtttga gcgtggtgac
    13081 gaagccgaag gatttttaat tgtcactgca gcagcagacc agggtctggt agatattcat
    13141 gaccgccgcc cacgagtact gtcgccagaa gctgcacggg aatggatgcg gcaagaggtt
    13201 ggcgataaag aagcctcaga aatcgcggcc agtggctgtg ttccagcaaa ccagttcacc
    13261 tggcacccgg tatcgtgcgc ggtgggtaat gttaaaaacc agggcgcgga gttaattcaa
    13321 cctgtttgat aagcctggag attatctaca cctggatgga catcattgta ctatgccccg
    13381 tacatgcccc atcacatcct caacatcaaa gtattagagc cttgcaaacc attatgcgtc
    13441 tcagtttgtc tcatctatct tacacaaaaa aaaatagaac taatatcaag cggcgctata
    13501 ttgc
//
LOCUS       DADTFG010000133        13498 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.134, whole genome shotgun sequence.
ACCESSION   DADTFG010000133 DADTFG010000000
VERSION     DADTFG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.134"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            234..1343
                     /gene="livk"
                     /locus_tag="J1138_003637"
     CDS             234..1343
                     /gene="livk"
                     /locus_tag="J1138_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBA8455389.1"
                     /translation="MKRNAKTIIAGMIALTISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1391..2317
                     /gene="livH"
                     /locus_tag="J1138_003638"
     CDS             1391..2317
                     /gene="livH"
                     /locus_tag="J1138_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBA8455390.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSAYALIAIGYTMVYGIIGMINFAHG
                     EVYMISSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2314..3591
                     /locus_tag="J1138_003639"
     CDS             2314..3591
                     /locus_tag="J1138_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HBA8455391.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3588..4355
                     /gene="livG"
                     /locus_tag="J1138_003640"
     CDS             3588..4355
                     /gene="livG"
                     /locus_tag="J1138_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBA8455392.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4357..5070
                     /gene="livF"
                     /locus_tag="J1138_003641"
     CDS             4357..5070
                     /gene="livF"
                     /locus_tag="J1138_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBA8455393.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5467..6783
                     /gene="ugpB"
                     /locus_tag="J1138_003642"
     CDS             5467..6783
                     /gene="ugpB"
                     /locus_tag="J1138_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBA8455394.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            6881..7768
                     /gene="ugpA"
                     /locus_tag="J1138_003643"
     CDS             6881..7768
                     /gene="ugpA"
                     /locus_tag="J1138_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBA8455395.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7765..8610
                     /gene="ugpE"
                     /locus_tag="J1138_003644"
     CDS             7765..8610
                     /gene="ugpE"
                     /locus_tag="J1138_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBA8455396.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8612..9682
                     /locus_tag="J1138_003645"
     CDS             8612..9682
                     /locus_tag="J1138_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HBA8455397.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLLLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            9679..10422
                     /gene="ugpQ"
                     /locus_tag="J1138_003646"
     CDS             9679..10422
                     /gene="ugpQ"
                     /locus_tag="J1138_003646"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBA8455398.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWHSKAFKGEPLPLLSQVAK
                     RCRKHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10409..10849)
                     /locus_tag="J1138_003647"
     CDS             complement(10409..10849)
                     /locus_tag="J1138_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBA8455399.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10969..12702
                     /gene="ggt"
                     /locus_tag="J1138_003648"
     CDS             10969..12702
                     /gene="ggt"
                     /locus_tag="J1138_003648"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBA8455400.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSNKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADTMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMIGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(12740..13024)
                     /locus_tag="J1138_003649"
     CDS             complement(12740..13024)
                     /locus_tag="J1138_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455401.1"
                     /translation="MITYHDAFAKANNYLDDANLPVVITLHGRFSQDWYFCFEAREFL
                     ETGDEAARLAGNAPFIVDKGSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(13224..13379)
                     /locus_tag="J1138_003650"
                     /pseudo
     CDS             complement(13224..13379)
                     /locus_tag="J1138_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3068 a         3370 c         3787 g         3273 t
ORIGIN      
        1 ccgttatttt atgctgacaa cggcgctttt ttctgtttat ctatcaataa attcagaata
       61 ttatctgttc ttaatcgact gaaaaatagg gattttaatc gctatcatca caaaatactg
      121 cgctaaaccc ttaatcagac aggtaaaaac agtgcagtat aaaaaaagaa cagtctgatt
      181 tgttaacaca taaaaacaaa gcaacacaac atcacgaatg gggatttttg actatgaaac
      241 ggaatgcgaa aactatcatc gcagggatga ttgcactgac aatttcacac accgctatgg
      301 ctgacgatat taaagtcgcc gttgtcggcg cgatgtccgg cccgattgcc cagtggggcg
      361 atatggaatt taacggcgcg cgtcaggcaa ttaaagacat taatgccaaa gggggaatta
      421 agggcgataa actggttggc gtggaatatg acgacgcctg cgacccgaaa caagccgttg
      481 cggtcgccaa caaaatcgtt aacgacggca ttaaatatgt tattggtcat ctgtgttcct
      541 cctctaccca acctgcatca gatatctatg aagacgaagg tattctgatg atctcgccgg
      601 gagcgaccaa tccggagctg acccaacgcg gttatcaata catcatgcgt actgccgggc
      661 tggactcttc ccaggggcca acggcggcaa aatacattct tgagacggtg aagccccagc
      721 gcatcgccat tattcacgac aaacaacagt atggcgaagg gctggcacgt tcggtgcagg
      781 acgggctgaa agcggctaac gccaacgtcg tcttcttcga cggcattacc gctggagaga
      841 aagatttctc cgcgctgatc gcccgcctga aaaaagaaaa catcgacttc gtttactacg
      901 gcggttacta cccggaaatg gggcagatgc tgcgccaggc ccgttccgtt ggcttgaaaa
      961 cccagtttat ggggccggaa ggtgtcggta atgcgtcgct gtcgaacatt gccggtgatg
     1021 ccgctgaagg catgttggtc accatgccaa aacgctatga ccaggatccg acaaaccagg
     1081 gcatcgttga tgcgctgaaa gccgacaaga aagatccgtc cgggccctat gtctggatca
     1141 cctacgcggc ggtgcaatct ctggcaactg cccttgaacg taccggcagc gatgagccgc
     1201 tggcgctggt gaaagattta aaagctaacg gtgcaaacac cgtaattggg ccgctgaact
     1261 gggatgaaaa aggcgatctt aagggattcg attttggtgt cttccagtgg cacgccgacg
     1321 gttcatccac ggcagccaag tgatcatcct accgtccgtg aaaaacgggc gggtttagaa
     1381 aggttacctt atgtctgagc agtttttgta tttcttgcag cagatgttta acggcgtcac
     1441 gctgggcagt gcctacgcgc tgatagccat cggctacacc atggtttacg gcattatcgg
     1501 catgattaac ttcgcccacg gcgaggttta catgattagt agctatgtct catttatgat
     1561 catcgccgca ctgatgatga tgggtattga taccggctgg ctgctggtgg cggcgggatt
     1621 cgtcggcgca atcgtcattg ccagcgccta cggctggagt atcgaacggg tagcctatcg
     1681 cccggtgcgt aactctaagc gcctgattgc gcttatttcc gcaatcggca tgtctatctt
     1741 cctgcaaaac tacgtcagcc tgaccgaagg ttcgcgcgac gtggcgctgc ccagcctgtt
     1801 taacggtcag tgggtagtgg gtcatagcga aaacttctct gcctctatta ctaccatgca
     1861 ggcggttatc tggattgtta ctttcctcgc catgctggca ctgacgattt tcattcgcta
     1921 ttcccgtatg gggcgagcct gccgtgcctg cgcggaagat ctgaaaatgg cgagcctgct
     1981 tggcatcaac accgaccggg tgattgcgct gacctttgtg attggcgcgg cgatggcggc
     2041 agtggcgggc gtgctgctcg gtcagttcta cggcgtaatt aacccctaca tcggctttat
     2101 ggccgggatg aaagccttta ccgctgcggt gctcggtggg attggcagca ttccgggggc
     2161 gatgattggc gggctgatcc tggggattgc ggaggcgctc tcttctgcct atctgagtac
     2221 ggaatataaa gatgtggtct cattcgccct gctgatcctg gtgctgctgg tgatgccgac
     2281 cggtattctg ggtcgcccgg aggtagagaa agtatgaaac cgatgcatat tgcaatggcg
     2341 ctgctctctg ccgctatgtt ctttgtgctg gcgggcgtct ttatgggcgt gcaactggag
     2401 ctggatggca ccaaactggt ggtcgacacg gcttcagaca tccgttggca gtgggtgttt
     2461 atcggcactg cggtggtctt tttcttccag cttttgcgac ctgctttcca gaaagggcta
     2521 aaaagcgttt ccgggccgaa gtttattctg cccgcgattg atggctccac ggtgaagcag
     2581 aaactgttcc tcgtggcgct gctggtgctt gcggtggcgt ggccgtttat ggtgtcgcgt
     2641 gggacggtgg atatcgccac tctgaccatg atctacatta ttctcggtct cgggctgaac
     2701 gtggttgttg gtctttctgg tctgctggtg ctgggatacg gcggctttta cgccatcggc
     2761 gcttacactt ttgcgctgct caatcactat tacggtctgg gcttctggac ctgtctgccg
     2821 attgccgggt tgatggcggc ggcggcgggc ttcctgctcg gttttccggt gctgcgtctg
     2881 cgcggtgact atctggcgat cgttaccctc gggttcggcg aaattgtgcg catattgctg
     2941 ctcaataaca ccgaaattac cggcggaccg aacggtatca gtcagattcc gaaaccgacg
     3001 ctcttcggac tcgagttcag ccgtaccgcc cgcgaaggcg gctgggacac gttcagcaat
     3061 ttctttggcc tgaaatacga tccctccgat cgcgtcattt tcctctatct ggtggcgttg
     3121 ctgctagtgg tgttaagcct gtttgtcatt aaccgcctgc tgcggatgcc gctggggcgt
     3181 gcgtgggaag cgttgcgtga agatgaaatc gcttgtcgtt cgctgggctt aagcccgcgt
     3241 cgtatcaagc tgactgcctt taccatcagc gctgcgtttg ccggttttgc tggaacgttg
     3301 tttgctgcgc gccagggctt tgtcagccct gaatccttca cttttgccga atcggcgttt
     3361 gtgctggcga tagtggtgct cggcggtatg ggctcgcaat ttgcagtgat tctggcggct
     3421 gttttgctgg tggtatcgcg cgagttgatg cgtgatttca acgaatacag catgttaatg
     3481 ctcggtggtt tgatggtgct gatgatgatc tggcgtccgc agggcttgct gcccatgacg
     3541 cgcccgcaac tcaagctgaa aaacggcgca gcaaaaggag agcaggcatg agtcagccat
     3601 tattatctgt taacggcctg atgatgcgct tcggcggcct gctggcggtg aacaacgtca
     3661 atcttgaact gtacccgcag gagatcgtct cgttaatcgg ccctaacggt gccggaaaaa
     3721 ccacggtctt caactgcctg accggattct ataaacccac cggcggcacc attttactgc
     3781 gcgatcagca tctggaaggt ttaccggggc agcaaattgc ccgcatgggc gtggtgcgca
     3841 ccttccagca tgtacgtctg ttccgtgaaa tgacggtgat tgaaaacctg ctggtggcac
     3901 agcatcagca actgaaaact gggctgttct ccggcctgtt gaaaaccccc tctttccgtc
     3961 gcgcccagag cgaagcgctc gaccgcgccg ctacctggct tgagcgcatt ggtttgctgg
     4021 aacatgccaa ccgtcaggcg agtaacctgg cctatggtga ccagcgtcgt ctggagattg
     4081 cccgctgtat ggtaacgcag ccggagattt taatgctcga cgaaccggca gcaggtctta
     4141 acccgaaaga gacgaaagag ctggatgagc tgattgccga actgcgtaac catcacaaca
     4201 ccactatctt gttgattgaa cacgatatga agctggtgat ggggatttca gacagaattt
     4261 acgtggtcaa tcaggggacg ccgctggcaa acggtacacc ggagcagatc cgtaataacc
     4321 cggacgtgat ccgtgcctat ttaggtgagg cataagatgg aaaaagtcat gttgtccttt
     4381 gacaaagtca gcgcccacta cggcaaaatc caggcgctgc atgaggtgag cctgcatatc
     4441 aatcagggcg agattgtcac cctcattggc gcgaacgggg cggggaaaac caccttgctc
     4501 ggcacgttat gcggcgatcc gcgcgccacc agcgggcgaa ttgtgtttga tgataaagac
     4561 attaccgact ggcagacggc gaaaatcatg cgcgaagcgg tagcgattgt cccggaagga
     4621 cgtcgcgtct tctcgcggat gacggtggaa gagaacctgg cgatgggcgg cttttttgcc
     4681 gaacgcgacc agttccagga gcgcataaag tgggtctatg aactgtttcc acgtctgcat
     4741 gagcgccgtg ttcagcgagc gggcactatg tctggcggtg aacagcagat gctggcgatt
     4801 ggtcgcgcgc tgatgagcaa cccgcgtttg ttgttgcttg atgagccatc gctcgggctg
     4861 gcaccgatta tcatccagca aattttcgac accatcgaac aactgcgcga gcaggggatg
     4921 accatcttcc ttgtcgagca gaacgccaac caggcgctaa agctggcgga tcgcggctac
     4981 gtgctggaaa acggtcatgt agtgctctcc gataccggcg acgcgctgct ggcgaatgaa
     5041 gcggtgagaa gtgcgtattt aggcgggtaa taacacgttg attgataagg agtcaaaaga
     5101 ctcctttgag acaggtgaca aatgtaaaat tgcctgatgc gctgcgctta tcaggcctac
     5161 tgggtgagtt gcaatatatt gaatttgcac gatcttgtag gccggataag gcgtttacgc
     5221 cgcatccggc atgaaacgat gagtaatctg tagagtttga ttcagacctt ctatttttcc
     5281 gcttatccgt gccccatctc ccattttccc tcgcccgcgc cgtcaccgcc ttgtcatctt
     5341 tctgacacct tactatctta aaaatgtaac aaaatagtta tttttctgta attcgagcat
     5401 gtcatgttac tccgcgagca taaaacgcgt gaattcgcgc attcggtaca acaagagaga
     5461 taatcaatga aaccgttacg ttatacagct tcagcactgg cgctcggact ggctttaatg
     5521 gcaaatgcgc aggcagtgac gaccattccg ttctggcatt ctatggaagg ggaactgggt
     5581 aaagaggtgg attctctggc ccaacgtttt aacgccgaaa acccggatta caaaattgta
     5641 ccgacctata aaggcaacta cgaacagaat ttaagcgcgg ggattgccgc atttcgtacc
     5701 ggcaacgctc cggctatttt gcaggtttat gaagttggca ccgccaccat gatggcgtcg
     5761 aaagccatta aaccggtgta tgacgtattt aaagaggcag ggattcaatt cgatgagtcg
     5821 cagtttgtgc cgacggtttc aggctattac tccgacagca aaaccggcca cttactctcc
     5881 cagccgttca acagctcgac tcccgttctc tattacaaca aagacgcctt caagaaagca
     5941 ggattagacc cggaacagcc gccgaaaacc tggcaggatc tggcggacta tgccgcaaaa
     6001 ctgaaagcct ccggtatgaa gtgcggctac gccagcggct ggcagggatg gatccaactg
     6061 gaaaacttta gcgcctggaa cggtttgccg tttgccagca aaaacaacgg ctttgacggc
     6121 acagacgcgg tgcttgagtt caacaagccg gagcaggtga aacacatcgc catgctcgaa
     6181 gagatgaaca agaagggcga tttcagctac gttgggcgta aggatgaatc caccgagaag
     6241 ttctataacg gtgattgcgc gatgacgacc gcctcttccg gttctcttgc caacattcgc
     6301 gagtacgcca aatttaacta tggcgtaggc atgatgcctt acgacgccga tgcgaaagac
     6361 gcgccgcaaa acgccattat cggcggagcc agtctatggg taatgcaggg taaagataaa
     6421 gaaacctaca ccggcgtggc gaagttcctc gacttcctcg caaagccaga aaacgctgcc
     6481 gagtggcatc agaaaaccgg ctatctgcca atcaccaaag cggcgtatga cctgacccgt
     6541 gagcagggct tttacgagaa aaacccagga gcggatattg ccacgcgtca gatgctgaac
     6601 aagccaccgt tgccgttcac caaagggctg cgtctgggca acatgccgca gatccgcgtg
     6661 attgtggatg aagagctgga gagcgtgtgg accggtaaga agacaccaca gcaggcgctg
     6721 gataccgccg ttgagcgtgg gaaccagtta ctgcgccgct ttgagcagtc tacgaagtct
     6781 taatcagtgt aatgtcggat gcgtttcgct tatctgacct ggcatcgcgt gtaggccgga
     6841 taagcgaagc gcatccggca cagttcagga attaaccgta atgtcatcat cccgtccggt
     6901 gttccgctcg cgctggctgc cttatttgct ggtcgcgccg cagctcatca tcaccgttat
     6961 cttttttatc tggcctgcgg gcgaagcgtt gtggtactcg ctgcaaagcg tcgatccgtt
     7021 tggtttctcc agccagtttg tcggcctgga taacttcgtc acgctgtttc atgacagcta
     7081 ttatctcgac tccttctgga cgacgataaa attcagcact tttgtcaccg tcagcggttt
     7141 gctggtatcg ctgttctttg cggcgctggt ggagtacatc gtgcgcggca gccgtttcta
     7201 tcaaacctta atgctgctgc cgtatgccgt ggctcccgcc gttgccgccg tattatggat
     7261 cttcctgttt aaccctggtc gcgggctgat cacccatttt ctcgctgagt tcggctacga
     7321 ctggaaccac gcgcaaaaca gcggtcaggc gatgtttctg gtggtgtttg cctcggtgtg
     7381 gaagcaaatc agctacaact tcctgttctt ctatgccgcg ctgcaatcca ttccccgttc
     7441 gttgatcgaa gccgcagcca tcgacggtgc aggcccgatt cgccgcttct ttaagattgc
     7501 gctgccgctt atcgctccgg tgagtttctt cctgctggtg gtgaacctgg tgtacgcctt
     7561 cttcgacacc ttcccggtga tcgacgccgc tacgtccggc gggccggttc aggccaccac
     7621 aacgctgatt tataagatct accgcgaagg ctttaccgga ctggatctgg cttcgtctgc
     7681 cgcgcagtcg gtggtgttga tgttcctcgt catcgtgctg acggtggtgc agttccgcta
     7741 tgttgaaagc aaggtacgtt accaatgatt gagaaccgtc cgtggctgac aatattcagc
     7801 cataccatgc tgatcctcgg gatcgcggtg atcctcttcc cgctgtacgt ggcgtttgtc
     7861 gcggcgacgc tggataaaca agaagtctat gccgcaccga tgacgctaat ccccggcaca
     7921 catctgctgg aaaacatcca caacatctgg gtaaacgggg taggcacgaa tagcgcgccg
     7981 ttctggcgga tgttgcttaa cagctttgtg atggcgttca gcattacgct cggcaaaatt
     8041 accgtctcga tgctctcggc atttgccatt gtctggtttc gttttccgct acgtaacctc
     8101 ttcttctgga tgatttttat caccctgatg ctgccggttg aagtgcgtat cttcccgacg
     8161 gtggaagtca tcgccaacct gaaaatgctc gacagctacg caggtttaac gctgccgctg
     8221 atggcctcgg cgaccgccac gtttctgttt cgccagttct ttatgaccct accggatgaa
     8281 ctggtggaag ccgcgcggat cgacggcgcg tcgccaatgc gtttcttttg cgacatcgtt
     8341 tttccgctgt ccaaaactaa tctggcggcg ctgtttgtga ttacctttat ttacggctgg
     8401 aaccagtatt tgtggccgtt gttgattatt accgacgttg atctcggcac caccgtggca
     8461 gggattaaag ggatgatcgc cacaggcgaa ggcacaacgg aatggaactc ggtgatggcg
     8521 gcgatgttgt taaccctgat cccaccagta gtgattgttt tagtgatgca gcgtgccttc
     8581 gtgcgcggcc tggtcgatag tgagaaataa gatggcagga ctgaaattac aggcagtaac
     8641 caaaagctgg gatggcaaaa cccaggtcat taaaccgctg acccttgatg tggcggatgg
     8701 cgaatttatc gtgatggtcg ggccgtctgg ctgcgggaaa tcgacgttat tgcgaatggt
     8761 tgccggactg gagcgggtaa cgacgggcga tatctggatc gaccgcaagc gtgtcaccga
     8821 aatggagccg aaagatcgcg ggattgcgat ggtgttccag aactacgcgc tctatccgca
     8881 tatgagcgta gaggagaaca tggcgtgggg gctgaaaatt cgcggcatgg gcaagcaaca
     8941 aattgccgaa cgtgttaaag aagcggcgcg cattctggag ctggacggcc tgcttaagcg
     9001 ccgcccgcgt gagctatctg gcggtcagcg tcagcgtgtg gcgatggggc gtgcgattgt
     9061 gcgtgagccg gcagtgttcc tgtttgatga gccgctctct aacctcgatg ccaagttgcg
     9121 tgtgcagatg cgtcttgaac tgcaacagct gcaccgtcgc ctgaaaacga cttcactcta
     9181 cgttacccac gatcaggttg aggcgatgac gctcgcccag cgagtaatgg tgatgaacgg
     9241 cggcgttgcc gaacagattg gcacgccagt tgaagtgtac gaaaagcccg ccagcctgtt
     9301 tgtggcgagt tttatcggca gtccggcgat gaatctgctg gcagggcgtg tgaataacga
     9361 aggtacgcat ttcgaactgg atggtggtat tacgctgctg ctaaacggtg gctaccgtca
     9421 gtatgccggg cgtaaaatga ccctcggcat tcgcccggaa catatagcgc taagctcgca
     9481 ggcagaaggt ggtgtgccgc tggtgatgga cacgctggag atcctcggcg cagataacct
     9541 ggcgcacgga cgctggggcg aacagaagct ggtggtgcga ctggcgcatc aggagcgccc
     9601 gacggcaggc agcacgctgt ggctgcatct gccggaaaat cagctgcatc tttttgatgg
     9661 tgaaacagga caacgagtat gagtaactgg ccttatcccc gcatcgtcgc ccatcgtggt
     9721 ggcggtaagc tggccccgga aaacactctg gcggcaatcg acgtcggggc aagatacggt
     9781 cataagatga tcgaatttga cgcaaagtta tcgaaagatg gcgagatctt cctgctccat
     9841 gacgacaatc ttgaacgcac cagcaacggc tggggcgtcg cgggtgaact gaactggcag
     9901 gatttactgc gcgtggatgc aggcagttgg cacagcaaag cgtttaaagg tgagccgctg
     9961 ccgttgcttt cgcaggtggc gaaacgttgt cgcaaacacg ggatgatggc gaatatcgaa
    10021 atcaaaccca ccactggcac tggaccatta acgggcaaaa cggtggcgtt ggcggcacgc
    10081 gaactgtgga ccggtatgac gccgccgctg ctgtcatcgt ttgagattga tgctttagaa
    10141 gccgcgcaac aggcagcacc ggaactgccg cgtggtttat tgcttgatga atggcgcgac
    10201 gactggcgag aattgaccgc gcggctgggc tgcgtctcta ttcatctcaa tcataagttg
    10261 ctcgataaag cgcgagtgat gcagttgaaa gacgccggat tacggattct ggtttatacc
    10321 gtcaataaac cccagcgcgc ggcagagttg ctgcgctggg gcgtggattg catctgtacc
    10381 gatgcgattg acgtgattgg tccgaacttt acggcccaat agttttcaac ggaatgtcag
    10441 gctgcggcgt gctgggctga ttcagcatgt cgccgtttct cctctccggc agcatatgct
    10501 gctgattcaa cgaactatcc ggattacggt tgctgtttaa catcccgccg ttggtattgg
    10561 gcagcatttg ctgccgggcg ggatttcgct cccccggctg tgactgcaac acccgctgag
    10621 aattattgtt tatctggttt tctaaatgct gctgttgcag ctgcgtttgc gttttcagtt
    10681 gctggttcag catccctttt tgctggattt gctgcgtctg catttgagtt tgcatccgct
    10741 gctggctggg gatctgatat cccggctggt tagggttgtt cagagtatta atgggctgtg
    10801 caaagccgac aaacggcagg agtgccgtca aaagcagaag tcgtttcatc gcgtatcctc
    10861 ctctgaagat attctttaag tttactcgct tcccggcaaa acgatgatta attcagagtt
    10921 atataccagg cttagctggg gttgcccctt aatctctgga gaataacgat gataaaaccg
    10981 acgtttttac gccgggtggc cattgctgct ctgctcacag gaagttgttt tagtacggta
    11041 gccgcgccac ctgtttctta tggtgtggag gaagatgtct tccatccggt acgcgctaag
    11101 cagggaatgg tggcgtctgt agatgctact gccactcagg tgggggtgga tatcctcaag
    11161 gagggcggga atgccgttga tgctgccgtg gcggtgggct acgcgctggc agtaacgcat
    11221 ccgcaggcag ggaatctggg cggcggtggt tttatgctga tccgctcgaa aaatggcaat
    11281 accacggcta tcgatttccg cgaaatggca cccgccaaag cgacccgcga tatgttcctc
    11341 gatgatcagg gcaacccgga cagcaacaaa tcactcactt cgcatctggc ttccggcaca
    11401 cccggtacgg tagcgggttt ctcgttggcg ctggataaat acggcaccat gccgttgaat
    11461 aaagtggtgc aaccggcgtt taaactggca cgcgatggtt ttatcgttaa cgacgcgctg
    11521 gctgacgatc tcaaaaccta cggcagtgaa gtgctgccga accacgaaaa cagcaaagct
    11581 atcttctgga aagagggcga accgctgaaa aagggcgaca agctggtgca ggcgaacctg
    11641 gcaaagagcc tggagatgat tgccgaaaat ggcccggacg aattctacaa aggcacaata
    11701 gcggagcaga tcgctcagga gatgcagaaa aacggcggcc tgatcacgaa agaagattta
    11761 gcggcctata aagcggtcga gcgcacaccg ataagcggcg attatcgcgg gtatcaggtt
    11821 tactccatgc caccgccatc ttccggcggg atccatatcg tacaaatcct caatattctg
    11881 gaaaacttcg atatgaagaa atacggcttt ggcagcgccg atacgatgca aatcatggca
    11941 gaagctgaga aatacgccta cgccgaccgc tcggaatatc ttggcgaccc ggattttgtc
    12001 aaagtaccgt ggcaggcgct gaccaataaa gcctatgcca aatctattgc cgatcaaatt
    12061 gatatcaaca aagcgaagcc gtccagcgaa attcgccccg gcaagcttgc gccttatgag
    12121 agtaatcaaa ctacccatta ctcagtggtg gataaagatg gtaacgcggt ggcggtgacc
    12181 tatacgctga acaccacctt cggtacgggc attgtcgcgg gcgagagcgg tattctgctt
    12241 aataaccaaa tggatgattt ctccgccaaa ccgggcgtgc cgaacgttta cgggctggtg
    12301 ggcggcgatg ccaacgccgt cgggccgaat aaacgcccgc tgtcgtcgat gtcaccaacc
    12361 attgtggtca aggatggtaa aacctggctg gtcaccggaa gcccaggcgg tagccggatt
    12421 atcaccactg tgctgcaaat ggtggtgaat agcatcgatt atggaatgaa cgtcgccgaa
    12481 gcgaccaatg cgccgcgttt ccaccatcag tggttgccgg acgagctgcg tgtcgaaaaa
    12541 gggtttagcc cggatacgat caagctgctg gaagcaaaag gtcagaaagt ggcgctgaaa
    12601 gaggcgatgg gcagtacaca aagcattatg attgggccgg acggtgagct gtacggtgca
    12661 tccgacccgc gctcggtgga tgatttaacg gcggggtatt aaggttagcg gccctcttag
    12721 tgggaagagg gccgtattgt cagggcaagc cgaaggtagc cttttttatt tcataatcct
    12781 gtagatattc ttccagtggc tttgccgttc ccagagaatg aatttcacca ctgcctttat
    12841 caacaataaa aggtgcgtta ccagctaagc gcgcggcctc atctccagtt tcgagaaatt
    12901 ctcgtgcttc gaaacagaaa taccagtcct ggctaaagcg tccatgtaga gtaatgacga
    12961 ccgggagatt tgcatcatca aggtaattgt tcgctttcgc gaatgcgtcg tgataagtaa
    13021 tcataattat aataaatatt attgtttagc gttatattat tattcctgcg ttatacattg
    13081 agcaaagacg cgttcatctt cgtcaaagat attattaaat gctggttttg aaaagagttc
    13141 atcggataag gtatagtcac aaaataagta tctgctaata ttttgtgatt tattttccag
    13201 aacgtcgaac cggatttgct catttaagat atcagaatct tatgattctg gtaaaattct
    13261 aaccaaatca acatgtcact aagatcatca tcgccattag ctatttcagt ttaaatagcg
    13321 actacatgtc ttcggtccat ctcacttaag gagtgtagtt ccgttgtaag tttttctata
    13381 gcttgtattg ataaatttcg atcaagaaat ttactgctgg taaactctaa aaagatggca
    13441 tggtttacaa tgattttggt taccttttta ttattatgaa caactgtcca tgatttcg
//
LOCUS       DADTFG010000134        13411 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.135, whole genome shotgun sequence.
ACCESSION   DADTFG010000134 DADTFG010000000
VERSION     DADTFG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.135"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(62..970)
                     /gene="galU"
                     /locus_tag="J1138_003651"
     CDS             complement(62..970)
                     /gene="galU"
                     /locus_tag="J1138_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBA8455402.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKVWLEEEMGIKK"
     gene            complement(1172..2185)
                     /gene="rssB"
                     /locus_tag="J1138_003652"
     CDS             complement(1172..2185)
                     /gene="rssB"
                     /locus_tag="J1138_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBA8455403.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2277..3182)
                     /gene="rssA"
                     /locus_tag="J1138_003653"
     CDS             complement(2277..3182)
                     /gene="rssA"
                     /locus_tag="J1138_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBA8455404.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3295..3753
                     /locus_tag="J1138_003654"
     CDS             3295..3753
                     /locus_tag="J1138_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBA8455405.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3803..4645
                     /gene="purU"
                     /locus_tag="J1138_003655"
     CDS             3803..4645
                     /gene="purU"
                     /locus_tag="J1138_003655"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBA8455406.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4805..4889
                     /locus_tag="J1138_003656"
     tRNA            4805..4889
                     /locus_tag="J1138_003656"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4839..4841,aa:Tyr,seq:gta)
     gene            4936..5067
                     /locus_tag="J1138_003657"
     ncRNA           4936..5067
                     /ncRNA_class="other"
                     /locus_tag="J1138_003657"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            5320..6519
                     /locus_tag="J1138_003658"
     CDS             5320..6519
                     /locus_tag="J1138_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBA8455407.1"
                     /translation="MPARQVCQNFFRDALAPFHKYRQNALLDATIALINGASLTLTSI
                     GRYLPGNAQVKNKIKRVDRPLGNESLHHDIPLIFRNIISMLTSKLSLCVIAVDWSGYP
                     SQEYHVLRASLICDGHSIPLLSWIVPSEKQQNAKIQQAFLNTLSEAVNPKARVIIVTD
                     AGFQNAWFRHIKSLGWDFIGRIRGNKQLHLARKGECWFRRQELQASNKPEYLGPGTLS
                     RAEYARCDGHFYLHKKEPKGRRNKRSRCGIARPSQIKDARSAAKEPWLIFSSTDDFKP
                     REIMKLYSRRMQIEQNFRDEKSERFGFGLRASYSCSAGRMLVLSLLATLSTIVLWLIG
                     YHAENQGLHLRYQANSIKTRRVISYLTLAENVLRHSPLILKRTVLSTILNHLTRTYQN
                     MVLVYYR"
     gene            complement(6572..7249)
                     /gene="narI"
                     /locus_tag="J1138_003659"
     CDS             complement(6572..7249)
                     /gene="narI"
                     /locus_tag="J1138_003659"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBA8455408.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7249..7959)
                     /gene="narJ"
                     /locus_tag="J1138_003660"
     CDS             complement(7249..7959)
                     /gene="narJ"
                     /locus_tag="J1138_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBA8455409.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(7956..9494)
                     /gene="narH"
                     /locus_tag="J1138_003661"
     CDS             complement(7956..9494)
                     /gene="narH"
                     /locus_tag="J1138_003661"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBA8455410.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9491..13234)
                     /locus_tag="J1138_003662"
     CDS             complement(9491..13234)
                     /locus_tag="J1138_003662"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBA8455411.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3247 a         3585 c         3294 g         3285 t
ORIGIN      
        1 agctataaaa aaacggcgtc gattgctcaa caccgtttcg tggaaaacac cgatacggat
       61 gttacttctt aatgcccatc tcttcttcaa gccagacttt aaattccgtg ccaagggtgt
      121 tatgacgaat accgtattca acgaaggcct gcatgtaacc taatttatta ccgcagtcat
      181 ggctcttccc tttcatatga taggcttcaa ccgtttcttt ttcgatcagc atatcaattg
      241 cgtcggtgag ctgaatttca tcaccagctc caggaggggt ttttgccagc aacggccaaa
      301 tatccgcgct aagtacgtaa cgacccacaa tagcgagatt agacggcgca acatccgctt
      361 ttggtttttc aaccacgcca accatcggta cgctttcacc cggcgctaat tcaacgcctt
      421 tgcaatccac aacgccatat gcggtcacat cagcaaccgg ttcaaccatg atctggctat
      481 gacccgtttc atcaaagcgg cggatcatct ctgccaggtt atcctgtgat aaatcggatt
      541 catattcatc cagaataacg tcaggcaaaa taacagctac cggttcatca cccactaccg
      601 ggtgagcaca caataccgcg tgtcccaggc ctttcgccag cccctgacga acttgcataa
      661 tagtcacgtg cggcggacaa atagactgca cttcatcaag cagttgacgt tttacacgtt
      721 tttccagcat tgcttccagt tcaaaactgg tatcaaagtg gttttcaata gagtttttag
      781 atgagtgagt aaccagcaca atttcagtaa tgccagccgc aatacattca ttcacgacgt
      841 attgaattaa tggcttatcg acaagtggca gcatctcttt cgggatggct ttcgtcgccg
      901 gcaacatcct ggttcctaat cccgctacgg ggataacggc ttttttgact ttcgtattaa
      961 tggcagccat ttaaattctc ctggactgtt cgtgttttga acgtgttcat atttctgtat
     1021 cgcatcccag tatatcagca ccccgaaggg gctaagttct gaaaaggagg cgagttagca
     1081 taattaagac aattacttat acgaatagcg acgatagtac caccagccga cattagcagg
     1141 taatgcaaat ttagcccgtg ttatcgtttg ctcattctgc agacaacatc aagcgcagcc
     1201 gaccaccggt tccccatatt tggcattgcc aggcatcgca tcgctggctc aattgattca
     1261 aataagcgtt acctaaagtg cctaacggaa caccattact gatttgcacc tgatgttcgc
     1321 cggtatttaa cgtcgcattc agacccgcag aaaccagaat gagatttttc agttcgcgat
     1381 gataatagcc aactaatagc ggaaactgcc ccggcagatt ggcctgacgg agtaaatggt
     1441 ttacctgctt caataacgcg cccaactctg gcaaccgttg attttggtgt gcaagctgtt
     1501 cctgtaataa tccgttaaac aatgcgcgta ataacaaggc agcaagtacg ccattatgtc
     1561 cagctcgggt gacatcaagg caataaaatg ccagatcgtt ttccgaaagt gcggcaatat
     1621 caagcaccag gccgggtttg tccgcggcaa ctaattgacg ataattaacc cggcattggg
     1681 aaatcacctg ctgaaccggc ggttgtagtt cctgtaataa tttcgccgct gcagcagggt
     1741 tatcaaccat tgcatcccag tcgcgaaaaa gcctttcctc ttcctcaacg cgggaattaa
     1801 acatgctggg atagagacag gcaaaaacca tctcgcgcaa gcgattcaga tctttaaccg
     1861 gtttcagcaa aacatcttca acacccagac gtaacgcttt ggcaatatct gccatatttt
     1921 cagtggcaga tatcaccaga actggtgtct ggtcacctct gttgcgtata tgttccagca
     1981 gtttaagccc gttcattcgt ggcatcgcga tatcacatat catcaggtct ggagtgaaac
     2041 ctcccagcaa ctcaagggca tccaccccat cagccgccag taccgttgtc gctcccaatg
     2101 aggaaaacca cgaatccaga agcgagcgaa atacctgctc atcttcaaca atgagaatct
     2161 gttttccgac caatggctgc gtcatgttct ctcccctgac tggctttact caatagtggc
     2221 acgctattgc cacctacgcc tgtcagaatt aacttaagtg tagataaaaa ttctggtcaa
     2281 atgttggtgc gtaccaacgg caaaagttcg tccattttct tttccactgc cagttgtccg
     2341 gccgcaatgg cagcgtgcgc gcgatggaaa tcaagcgtag atatttgcgg gcaaacaggt
     2401 tgaatcagaa tatcgggcgg atcacctgcc atgcggttcc ttttaaggcg gttctccagc
     2461 acctggatag aagtggtcat aatctctgtt gccgttggcg ctgtcaccgc ccgacgtgtc
     2521 gttatgctgc ctaacctttc tttcagacgc gcatgccacg gcagagaatc accattctcg
     2581 ctattttctt cactgacatt aaaggagagc aaatcttgtt gcatcaaatg agcatcgtgc
     2641 tgcaggtcga ccgctatcac aatatcagcc cccaatgcac gcgtgaggga aataggaatt
     2701 gggttaacta cagcgccatc aaccagccag tagccgttat gcgcaacggg tgccattagt
     2761 cctggaatac tgcatgatgc acgaatagca agatggagat cgccttcagt aaaccataat
     2821 tcacgcccag tacttaaatt ggtggcaaca gccgcgaagc gacgggaaca attttcgatc
     2881 tctgtttccg gcattatttc gcgatattga ttgaagacac gctcgccgcg cagtaaccca
     2941 ccgcgctgcc aggagagatc catcaggcgt aaaacatccc aataactgaa agaggtcacc
     3001 caatcttcca gcgcagataa tcgatcgcat gcataggcag cgcccaccag cgaaccaatt
     3061 gaacatcctg caacgatatc aatttcaata cccacttttt ttagcgcatt aattacgcca
     3121 atatgcgacc aacctcttgc cgcgccagat cccagcgcca gccctatttt tatctttctc
     3181 attattcccg caagattccc ctggaatgca atcgtagcca catcgctact gctcggttaa
     3241 catagtgcta ctcaggcggt taatacgccg ttattgtttc cagggagatt atttgtgtct
     3301 cagctttgtc cctgtggtag tgctgtcgag tatagcctat gttgccaccc ttatgtgtct
     3361 ggtgaaaagg ttgcacctga tccagaacat ctcatgcgtt cgcgttactg cgcttttgtg
     3421 atgcaagacg cagattattt aataaagacc tggcatccat cttgtggagc agcggcgtta
     3481 cgagccgaat tgattgccgg atttgcgcat actgaatggt tgggattgac cgtatttgaa
     3541 cattgctggc aggacggcgg caatatcgga tttgtcagtt ttgttgctcg atttaccgag
     3601 ggtggcaaaa caggtgcaat aattgaacgt tcgcgatttt tgaaagaaaa cggtcaatgg
     3661 tactatattg acggtacacg tccgcagttt ggccgcaacg atccctgccc ttgtggttca
     3721 ggtaaaaaat ttaaaaagtg ctgcggccaa taatggttga cggtacggtt tagcaaacac
     3781 tctcaacaag gtttttccag caatgcattc actccaacgt aaagttctgc gtactatttg
     3841 tccggaccaa aaaggtctga tcgcacgtat taccaatatt tgctacaagc acgagttaaa
     3901 tatcgtgcag aacaatgaat ttgtagatca tcgtaccggg cgctttttta tgcgcacgga
     3961 actggaaggg atttttaatg attccaccct gctggcggat ctcgatagcg cattgccaga
     4021 aggctccgtg cgcgagctga atcctgccgg tcgtcgccgg atagtgattc tggtcactaa
     4081 agaagcgcat tgccttggcg atttgttgat gaaagccaat tacggcggcc tggatgtcga
     4141 aatcgcggca gttattggta accacgatac tttacgttct ctggttgagc ggtttgatat
     4201 tccgtttgag ctggtaagcc atgaagggtt aagccgcaac gagcacgatc aaaagatggc
     4261 ggatgccatt gatgcttatc aacctgacta tgtggtgctg gcgaagtata tgcgggtatt
     4321 aacgccggaa tttgtggcac gcttcccgaa taagatcatc aatattcacc attcattcct
     4381 gccagcgttt atcggcgcac gtccttatca ccaggcctat gagcgtggcg tgaagattat
     4441 tggcgcaacc gctcactatg tgaatgacaa tctggacgaa ggcccaatca tcatgcagga
     4501 cgttattcat gtcgatcata cctacacagc tgaagatatg atgcgcgcag gtcgtgacgt
     4561 cgagaaaaac gtcttaagtc gtgcgctata caaagtactg gcgcagcgcg tctttgttta
     4621 cggtaatcgg acgattattc tttaatcgcc agcaaaaata actggttagc tttaatccgt
     4681 tacggatgaa aattacgcaa ccagttcatt tttctcaacg taacacttta cagcggcgcg
     4741 tcatttgata tgatgcgccc cgcttcccga taagggagca ggccagtaaa aagcattacc
     4801 ccgtggtggg gttcccgagc ggccaaaggg agcagactgt aaatctgccg tcatcgactt
     4861 cgaaggttcg aatccttccc ccaccaccat cgctttcaaa agcccctgaa ttctcaaacg
     4921 aatccgcaat tgaatattct gcccatgcgg ggaaggatga gaagcttcga ccaaggttcg
     4981 actcgagcgc cagcgagaga gcgttgccgc aggcaacgac ccgtagggcg aagcgcgcag
     5041 cgctgagtaa tccttccccc accaccattt cttcctgaaa tactcaaaac ctgttactcc
     5101 gagctaattc tgcccaatgg caccgcgttt accggaccta aaatatcctc ccctgtaggt
     5161 cggataagcg aagcgcatcc gacaactccc cccgcaccgt gcccataaaa aaaacccgcc
     5221 cattactgag gaatccccag taatgggcgg gtaaaaaaag acaggcatag agttttgtga
     5281 tctactgact tgtgcaacca attcaatgag atctcctcta tgcctgctcg ccaagtatgt
     5341 cagaattttt tccgcgatgc tttagcacca tttcacaaat accgacaaaa tgctttgttg
     5401 gatgccacta tcgcactaat caatggcgca tctctgacgc tgaccagcat cggacgttat
     5461 ttacccggta atgctcaggt caaaaataaa ataaaacgtg ttgatcgtcc gctcggtaat
     5521 gagtctcttc atcacgacat ccccctgatt ttcagaaaca ttatttccat gctgacgagc
     5581 aaactgtcac tgtgtgtcat cgccgttgac tggagtggat acccctctca ggagtatcac
     5641 gtactccgcg ccagcctgat ttgcgatgga cattcgattc cattgctgag ttggatagtt
     5701 ccttcggaaa aacagcaaaa tgcaaagata cagcaagcct ttcttaatac tctttccgaa
     5761 gcggtaaacc caaaagcaag ggtaatcatc gtcacggatg cagggttcca gaatgcctgg
     5821 tttcggcaca taaaatcact cggctgggat tttattggcc ggatacgagg caataaacaa
     5881 ctgcatctgg cgagaaaagg ggaatgctgg ttcaggcgtc aggaattaca ggccagtaac
     5941 aaacctgaat atctggggcc ggggacgctt tcccgggcgg aatacgcccg ttgcgatggt
     6001 catttttatt tgcacaaaaa agagccgaaa ggccggagaa ataaacgttc gcgttgtggt
     6061 atagccagac catcacagat aaaagatgcc aggtcggcgg caaaagaacc atggttaata
     6121 ttcagcagca cagatgactt taaaccgcga gaaatcatga agttatacag ccgccgaatg
     6181 cagattgagc aaaacttcag ggatgaaaaa agtgagcgtt ttgggttcgg tctgcgagcc
     6241 agttacagct gttcggcagg aagaatgctg gtactgagcc tgctggcaac actgagcaca
     6301 atagtgctgt ggcttattgg ctatcacgct gaaaatcaag ggttacatct tagatatcag
     6361 gccaacagca ttaaaacgcg gcgggtaatc tcgtatctga cgttagcgga gaatgtctta
     6421 cgacactccc cgctaatttt aaaacgaacg gtgctaagca ccattcttaa ccacctcacc
     6481 agaacctacc agaatatggt gttggtttat tatcgctgat ttatggggat ccctcagcgc
     6541 cgaagcaggg tctatgtgaa ctaaaattcg cttagtgacg agcgcgcacc agctgatact
     6601 tacgcgtcag atactccacc gggacgctcc agatgtgtac cagacgcgag aacgggaaca
     6661 gcaggaataa cgtcatcccc aacaccaggt gcagacggaa gataaacgcc acgccatcaa
     6721 gatgttgaga agcgccaccg tggaaggtca ctaccgactg cgcccagcca accagtttca
     6781 tcatctcgct accgtccata tgctgggcgg agaacggaat ggtcagcagg cccagcgcgc
     6841 actggataac gagcagcgac aggatcagga tatccgctcc ggtagtcgtt gcacgcacgc
     6901 gtgggctgaa cagacgacgt ttcaacaaca gcacgccgcc aatcagacac agtacgccgc
     6961 tggcaccacc agcaaacatc gccattttct gttttacttc aatcggcagc caggcttcat
     7021 acatccagtg cggcgtcagc ataccgaaga agtgaccgac aaaaatcccc agaataccga
     7081 tatggaacag gttcgacgcc agattcatcc ctttgcgatc cagcatctgg ctggacgccg
     7141 cacgccatgt gtactgcccg tagtcataac gcagccagct accaatcagg aagaccgccc
     7201 cggctatgta cgggtagata tcaaagaaga acatattcag gaattgcatt agtgctgtcc
     7261 tccggtggtg atattcagat attgcggcgc gacggcaccg gcaaagcgac gctgatgagc
     7321 agtgattgct gaatcaccgc agcctttttc agcaaagaat ttaacctgct cttcttccca
     7381 gactgcatcc agcgcctgcg gcgtatcgtc gcgcgcttca tcggcaattt tttccgccac
     7441 tttgtcgctg tcgattgcgg tattcgccag tttcagcagc agatcaaaca gcacggcata
     7501 acggctttca cgctgttgca gacgcgcgct cagcaatgcc agaatcggcg caatatcttt
     7561 caaaccctcc acggcttcgc tttgcggcag ctgcgccagg tactccagat acagcggcag
     7621 atggtcgggc aattcgcggc tgtttaactg caagccgtgc tgctcgtact gcgccagcag
     7681 atccaccatc gcctggccgc ggtcacggga ttcaccgtga acatgttcaa atagcaacag
     7741 tgacgtggcg cggccacggt cgaacagttc gctgtactgc gcctgggcat cgagcggatc
     7801 catcgtcgtt aaatcgcgca ggaaaatgcc cagcgcatgg gcatcctctt ttggcagatt
     7861 tttcgacgcg gcaatcgcct caaacatctc ttgttgatgc tgccataagg cagcatccgg
     7921 atattcaagg agacgcgata caatcacgag ttcgatcatg ggtgcggctc cgttttgctg
     7981 gttacatcga tggcatcgat acggcggctg ttgaacagat tgaatttggt atctgaaccg
     8041 tggcagccat caccaaaggt aaagccgcag ccatttttct ccgggaaggc ttcccgcgcc
     8101 agttcacgat gactgctcgg caccacaaag cgatcttcgt agttggcaat agccagataa
     8161 cgatacatct cctgtgcctg ggcttcggtc agaccgacct cttccagcgc acgggtatcg
     8221 actttaccgt caacggtttc agcacgtttg taatgacgca tcgccagcat acgtttcagc
     8281 gcgcgcagta ccggtttggt atcaccggcg gtcagcagat tcgccagata ctgtaccgga
     8341 atacgcaggc tttcgacgtc tggcagaatg ccgttgctgc ccagttcacc cgcgtctgcc
     8401 gcagactgaa tcggcgacag aggcggcacg taccagacca tcggcagtgt gcgatattcc
     8461 ggatgcagcg gcagcgccag tttccattcc atcgccattt tataaactgg cgactgctgc
     8521 gcagcctcaa taacgctcag cggaataccg tctttaatcg cctgctcgat gactttcgga
     8581 tcgttcggat cgaggaacac atccagctga cgctggtaga gatctttctc gttctcggta
     8641 cttgccgcac gttcaatcgc gtcggcatcg tacagcagca cgccaagata acggatacga
     8701 ccgacacagg tttctgaaca tattgtcggc tggccggctt caatacgcgg atagcagaag
     8761 atgcacttct cagacttacc gctcttccag ttgaagtaga tttttttgta cgggcatccg
     8821 gtgatgcaca tacgccagcc acggcattta tcctggtcga tcaggacgat gccatcttct
     8881 tcacgcttgt aaatcgcacc gctcgggcag gtcgccacac atgccgggtt cagacagtgt
     8941 tcgcacaggc gcggcaaata catcatgaag gtgttttcga actggctata catcgccttc
     9001 tggatgttgt cgaagttctt gtctttcgcc agcttgtcaa actcaccgcc caggtcatct
     9061 tcccagttcg gccctttttc gattttcgcc atccgttcgc cggtaatcag tgaacgcgga
     9121 cgggcaatcg gctgtgattt gctgccttcc ggcgcggtat gcaggttctg ataatcaaaa
     9181 tcgaacggct cgtaataatc gtcgatcccc ggcagatgcg ggttagcgaa gattttaccc
     9241 agcagcatgg cacggttacc catgcgcggc tgcagtttgc cgttgatttt acggatccag
     9301 ccgcctttgt atttttcctg gttttcccag tcagtcggga agccctggcc cggcttggtt
     9361 tccacgttgt tgaaccacgc gtattccacg ccttcacggc tggtccagac gtttttacag
     9421 gtcactgaac aggtgtggca gccgatgcac ttatcgagat tcagcaccat gccgacttgt
     9481 gaacgaattt tcattttacg ctctcctgta cctggtcatt gccttcgcca tctaaccagt
     9541 caatgttctt catcttacgc actacaacaa actcatcgcg gttagaacct acggtgccgt
     9601 agtagttaaa gccatatgcc agatgggcat agccgccgat catatgcgtc ggtttcggcg
     9661 tgatacgggt gaccgagtta tggataccac cacgctgttg ggtaatttcc gaaccaggca
     9721 ggttaacgat acgttcctgc gcgtggtaca tcatggtcat ccctgccgga acacgctggc
     9781 tgacaaccgc acgggcagtc agtgcaccgt tgctgttgaa gacttcaatc cagtcgttat
     9841 cggcgatacc cagctctttg gcatcggctt cactcagcca gaccaccgga ccaccgcgac
     9901 ctaaagtcag catcagcagg ttgtcgctgt aggtggagtg gataccccac ttctggtgtg
     9961 gcgtcaggaa gttgagcgct ttttccgggt tgccattgga tttctggccc atcacttctt
    10021 tcaccgaacg ggtgtcgatc ggcggacgat aaaccagcag gctttcaccg aaatcacgca
    10081 tccattggtg atcctgatac agctgctgac ggccagagag cgtacgccac gggatcagct
    10141 cgtgaacgtt ggtgtaaccg gcgttgtaag aaacgtgttc atcttcaagg ccagaccatg
    10201 tcgggctgga gatgattttg cgcggctgtg cctgaatatc gcggaagcga atcttctcgt
    10261 cttctttatt cagcgccaga tgcgtatggt cacggccggt aaactcgctc agggcagccc
    10321 aggcttttac agctacctga ccattggttt ccggtgccag cgtcaggatc atctctgccg
    10381 catcaattgc ggtattgagc atcggctggc cttttgccgg accttccgct ttggtgtagt
    10441 tgagcttacg caggagatcc atctcgctct gggtgttcca ggcaatccct ttaccgccgt
    10501 taccgatttt ctccatcagc gggccgatag aggtaaagcg ttcgtaagtt gctggataat
    10561 cgcgctctac gaccataatg tgcggtgcag ttttacctgg gatcaggtcg cattcgcctt
    10621 ttttccagtc tttcacatcc agcggttgcg ccagttcagc ggcagagtcg tgctggatag
    10681 gcagcgtgac gatgtcagtt tctttaccca gatgaccaac gcacacttcg gagaatttct
    10741 tcgcgatggc tttgtagatt tcccagtcgc ttttcgcttc ccaggccgga tcgaccgccg
    10801 cagacagcgg gtgaataaac ggatgcatat ccgaagtatt catgtcgtct ttttcgtacc
    10861 aggtcgccgt cggcaaaatg atgtcggaat agagacaggt gctcgacaga cggaagtcca
    10921 gcgtcaccac cagatccagc ttgccttcca gaccgttgtc ctgccagtcc acttcttccg
    10981 gcttcacgcc gccctgctga cccaggtctt tgccctggat accgtgctcc gtccccagca
    11041 ggtacttgag cataaactca tgacctttac cggaagaacc gagcaggtta gaacgccaga
    11101 tgaacaggtt acgcgggtgg tttttaccgt tttccggctg ttccgcagcg aaacggatag
    11161 aaccctcttt cagggatttc accgtatagt ccaccggatt catcccggct ttttctgctt
    11221 cgcgagcgat agtcagcggg ttagtgccta actgcggcgc agacggcagc cagcccatgc
    11281 gttctgcacg gacgttaaag tcgatcaagt gtccggtata gcgagattta tccgccatcg
    11341 gcgacagcag ttcttccgca gtcacggttt cataacgcca ctggctggag tggttataga
    11401 aataagaggt actgttcatg tgacgcgccg gacgctgcca gtcgagggca aacgccagcg
    11461 gctgccagcc ggtttgcgga cgcagttttt cctgacctac atagtgcgcc cagccaccgc
    11521 cgctctgacc gacacagccg cagaaaatca gcatgttgat cagaccacga tagttcatgt
    11581 ccaggtgata ccagtggttc agcccagcgc cgacgataat catcgaacga ccgtgcgttt
    11641 tatcagcgtt atcggcaaat tcacgggcga tgcgaataat ctggctgcga gaaacgccgg
    11701 taatctgctc tgcccaggca ggggtgtaag ctttcacatc gtcatagctg gttgcacagt
    11761 taacgtcgtt caggccacgt tccagaccgt agtttgccag cgtcagatca taaacggtgg
    11821 tcaccagggc ggtgctgcca tcagccagtt gcaggcgttt caccggcagc ttatgcagca
    11881 gcacgttttc cagttccact ttgttgaagt gttccgtgcc atcgccacca aagtacggga
    11941 agcccacctc agcgatctca tcctggctac ccagcaggct cagttgcagc tcggtttctt
    12001 cgccagtttt gccgtcgcgc tgctcaagat tccatttgcc cttctcgccc cagcggaagc
    12061 caatagaacc gttcggcgca accatttcac cattggtatt aaaggcgaca gttttccatt
    12121 ccggattgtt ttcctggccc agtgcatcca ccagatcagc agcgcgcagc atacgacccg
    12181 cagcgtagta accgtcgcgc tcttccagca tcaccagcat cggcatgtcg gtgtagcggc
    12241 gcacatagtc ggtgaaatac tggcttgggt tgtcgaggtg gaattcacgc agcattacgt
    12301 gacccattgc cagcgccatt gccgcatcgg tgccctgttt cggtgccagc cacagatcgc
    12361 acagtttggc gatttcagcg tagtctggtg tgacggcaac agttttggtt cctttgtaac
    12421 gcacttcagt aaagaagtga gcatccgggg tacgcgtctg cggcacgttt gaaccccagg
    12481 cgatgatgta gctggagttg taccagtcag cagattccgg tacgtcagtt tgctcacccc
    12541 aggtttgcgg agacgcagga ggcaggtcgc agtaccagtc gtagaagctt aagcaagtac
    12601 cgccaatcag cgagagatag cgcgcacccg atgcgtaaga aaccatcgac attgccggaa
    12661 ttggcgagaa gccagcgaca cggtccgggc cgtaattttt gatggtgtaa acgttagatg
    12721 cggcgatcag ttcgttcact tcctgccagg aagaacgaac aaaaccaccg cgtccacgcg
    12781 cctgcttaaa gcttttcgct ttatcggcgt cttcaatgat agaagcccat gcctcaaccg
    12841 gatcgctatg cagcgccttc gcttcacgcc acattttcat caggcgtttg cgcatcatcg
    12901 ggtatttcag gcggttagcg ctgtatagat accaggagta gctggcaccg cgcgggcagc
    12961 cgcgaggttc atggtttggc aggtccgggc gagtacgcgg atagtcagtc tgctgggttt
    13021 cccaggtgac cagaccgttt ttgacgtaga ttttccagct gcaggagccg gtgcagttta
    13081 ccccgtgggt agagcggacg attttgtcat gctgccaacg ctggcgatat ccatcctccc
    13141 agtcacggtt ggtattgaga agctggccat gcccatcggc aaaggtttca cccttctgtt
    13201 tgaagtagcg aaaccggtcc aggaatttac tcatcggttt tctcctgtgg gagcctgtcg
    13261 gccctctgat aaatcgacat tgctaatgtt taagggcaag gtaacgctct gaaacagcgt
    13321 gggaattgat aacgatcaag agtgatgggg atgaaaaata aagtaaattc ctggaatacc
    13381 cctttaggat aattaacatt tatagcataa c
//
LOCUS       DADTFG010000135        13240 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.136, whole genome shotgun sequence.
ACCESSION   DADTFG010000135 DADTFG010000000
VERSION     DADTFG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.136"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..628
                     /gene="csy1"
                     /locus_tag="J1138_003663"
     CDS             <1..628
                     /gene="csy1"
                     /locus_tag="J1138_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HBA8455412.1"
                     /translation="WHSQPDIRFPNLAEMHFGGTKPQNISLLNSVRGGRVWLLPSMPP
                     AWGPLDRAPQKMHSIFALRGDFNRAASGIIARMTYLLKVDTNNVHIRTVRAKYIDELI
                     DLLFMQASAFQQEKWQGWSAQSPDLPRHQQLWLDPWRSLSDETFKQEREKNDWQVTVA
                     DDFARWLNYRLKKSSFDVGAVEQKEWRSQSLFAQRMREMEAVLQEALK"
     gene            625..1548
                     /gene="csy2"
                     /locus_tag="J1138_003664"
     CDS             625..1548
                     /gene="csy2"
                     /locus_tag="J1138_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HBA8455413.1"
                     /translation="MSYLLLLPHIRIENANAVSGLTWGFPSMTHFLGYVHALSRKVVD
                     EFGVSFDGCAVVSHEQHIQAYSSGRDFQFALTRNPLTREGKTASFNEEGRMHLTVSLL
                     IECNGEITNGEYGRKVLCDYLKMLCQSHKLAGGSIVSMRDPQLFHAPEDEKQLRKIVW
                     RLMPGYALYDRSEWLAEHHQQHPDISLLDAWLDFAAIKYQAESPAEDNSAKWVYQPKP
                     MPGFLVPLMCGYQRISPVYAPGEVENARDTVTPFAFAEAVYGIGEWRGLHRTTDLQAL
                     MWRYRTTDTGYYCSATPVVDDFTFNEYDDLE"
     gene            1559..2560
                     /gene="csy3"
                     /locus_tag="J1138_003665"
     CDS             1559..2560
                     /gene="csy3"
                     /locus_tag="J1138_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HBA8455414.1"
                     /translation="MAKAPVAIKTASVLAFERKLATSDAVMYAGNWQGNEWQPIEIQE
                     KAVRGTISNRLKNAIVSDPTKLDAEIQKANLQRVDVASLPVDTDTLKVVFTLRVLGNL
                     STPSVCNDMAYQEALSGVIEGYISEHGFKELTLRYALNLANGRFLWRNRIGAEQIQVK
                     VTANETSWTFNSHDFSLRQFDQGQAAVTQLAAIIEQGLSAKEWVMLTVEAQVRLGAGQ
                     EVFPSQELVLDSNSSKSRVLYQVAGIAGIHSQKIGNALRTIDTWHPKVDELGAIAVEP
                     YGSVTSRGVACRQPKEKLDFYTLLDNWVTKGMKPDVEQQHYVMAVLIRGGVFGEKSE"
     gene            2571..3125
                     /gene="cas6f"
                     /locus_tag="J1138_003666"
     CDS             2571..3125
                     /gene="cas6f"
                     /locus_tag="J1138_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HBA8455415.1"
                     /translation="MDHYLEIRVLPDPEFSSEMLMAALFAKLHRVLGARGQGDIGVSF
                     PDVNVMPGARLRLHGSAQALQALEASTWRKGLTDYCQCSPVTPVPEIKGWRVVSRVQV
                     KSNPQRLLRRSVKKGWLTEEQAIERLATQAEQRTDLPFLNMKSLSSQQLFKLFIRHGD
                     LLKEPVKGEFSSYGLSATATIPWF"
     gene            complement(3236..3323)
                     /locus_tag="J1138_003667"
     tRNA            complement(3236..3323)
                     /locus_tag="J1138_003667"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3287..3289),aa:Ser,seq:gga)
     repeat_region   3666..3874
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3726..3753
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            complement(3987..4205)
                     /gene="infA"
                     /locus_tag="J1138_003668"
     CDS             complement(3987..4205)
                     /gene="infA"
                     /locus_tag="J1138_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBA8455416.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(4490..5194)
                     /gene="aat"
                     /locus_tag="J1138_003669"
     CDS             complement(4490..5194)
                     /gene="aat"
                     /locus_tag="J1138_003669"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBA8455417.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(5236..6957)
                     /gene="cydC"
                     /locus_tag="J1138_003670"
     CDS             complement(5236..6957)
                     /gene="cydC"
                     /locus_tag="J1138_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBA8455418.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(6958..8724)
                     /gene="cydD"
                     /locus_tag="J1138_003671"
     CDS             complement(6958..8724)
                     /gene="cydD"
                     /locus_tag="J1138_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBA8455419.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIISQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGIDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNTASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(8847..9812)
                     /gene="trxB"
                     /locus_tag="J1138_003672"
     CDS             complement(8847..9812)
                     /gene="trxB"
                     /locus_tag="J1138_003672"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBA8455420.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHINRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10357..10851
                     /gene="lrp"
                     /locus_tag="J1138_003673"
     CDS             10357..10851
                     /gene="lrp"
                     /locus_tag="J1138_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBA8455421.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            10986..>13240
                     /gene="ftsK"
                     /locus_tag="J1138_003674"
     CDS             10986..>13240
                     /gene="ftsK"
                     /locus_tag="J1138_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBA8455422.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPTRPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPV"
BASE COUNT         3403 a         3319 c         3421 g         3097 t
ORIGIN      
        1 atggcactcc cagccagata tccggtttcc taatcttgcc gaaatgcatt tcggcggcac
       61 aaaaccgcag aatatttctt tgctaaacag tgtgcgtggt gggcgcgtct ggctgttgcc
      121 gtcaatgccg cctgcctggg ggccgctgga tcgcgcacca caaaaaatgc acagtatttt
      181 tgccttacgc ggcgatttca atagggcggc ttcaggcatc atagcccgga tgacttacct
      241 gctgaaggtt gacacaaata atgttcatat ccgaactgtg cgggcgaaat atattgatga
      301 gttgatcgat ttgctgttca tgcaggcatc cgcttttcag caagaaaaat ggcagggatg
      361 gtccgcacaa tcaccagact tacctcgcca ccaacaactt tggctggacc cgtggcgaag
      421 cctctcagat gagactttca aacaggagcg tgagaaaaac gactggcaag taactgtcgc
      481 cgatgatttc gcacgctggc tgaactatcg cctgaaaaaa tccagttttg acgtaggcgc
      541 tgtcgaacaa aaagagtggc gcagccagtc tctgttcgct caacggatgc gtgaaatgga
      601 agctgttttg caggaggcgc tgaaatgagt tatctgctct tgttacccca tatacgcatc
      661 gaaaacgcta acgctgtctc cgggttgacc tggggattcc cgtcgatgac ccattttctc
      721 ggttatgtcc atgcgctttc tcgcaaagtc gtggatgaat ttggcgtaag ttttgatggt
      781 tgtgcggtag taagccatga acagcatatt caggcgtaca gctctgggcg cgattttcag
      841 ttcgctctca cccgtaaccc attaaccaga gaaggtaaaa ccgcctcgtt caatgaagag
      901 ggccgcatgc acttgacggt ttctctactg attgaatgta atggcgaaat caccaatggt
      961 gaatatggca gaaaagttct ttgtgactat ctgaaaatgc tttgccagag ccacaaactg
     1021 gcgggcggca gcattgtcag tatgcgcgat ccgcagcttt ttcatgcgcc tgaagatgaa
     1081 aagcaactgc gtaaaatcgt ctggcggtta atgccgggtt atgcgcttta cgatcgcagt
     1141 gaatggctgg ctgaacatca ccaacaacat cccgacatct cgttgctgga tgcctggctt
     1201 gatttcgccg cgattaaata tcaggccgaa tctccggctg aagacaacag cgcaaaatgg
     1261 gtataccagc cgaagccaat gcctggtttc ctggtaccgt taatgtgtgg ctaccaacgc
     1321 atttctcctg tttacgcacc cggtgaagtt gaaaatgcgc gcgacaccgt gacgccattt
     1381 gcctttgccg aagcggtata tggcattggc gaatggcgcg gattacatcg cactacagac
     1441 ttgcaagcgc tgatgtggcg ttaccgcaca acggacactg gctactattg ttcggcaacg
     1501 ccagttgttg atgattttac ctttaatgaa tatgacgatt tagaataagg acataattat
     1561 ggcaaaggct cctgtagcaa taaaaacggc gtcggtactg gcatttgagc gcaaactggc
     1621 aacctctgac gcggtaatgt acgcaggtaa ctggcaagga aatgaatggc aacccattga
     1681 gattcaggag aaagcggtcc gcggaaccat ctctaatcgc ctgaaaaatg ccattgtcag
     1741 cgatcccaca aaactggatg ctgaaatcca aaaagccaac ctgcaacggg tggatgtggc
     1801 ttcgcttccg gtagatacgg atacgttaaa ggtggtgttt acgctgcgtg tgctgggtaa
     1861 tttatccaca ccgtcagtat gtaatgatat ggcttaccag gaggcgcttt ccggggtcat
     1921 tgaaggttac atcagcgaac atggctttaa agagctgacg cttcgctatg cgctgaatct
     1981 ggcaaatggt cgttttctgt ggcgcaaccg cattggtgcc gaacagatcc aggtgaaagt
     2041 gactgctaac gagacgtcat ggacttttaa cagccatgac ttctctttac gtcaatttga
     2101 ccagggacag gcagcggtca cgcagctggc ggccattata gagcaaggat taagcgcaaa
     2161 agagtgggtg atgcttaccg ttgaagcgca ggttcgtctt ggtgccgggc aagaagtttt
     2221 tccgtcgcag gagctggtac tcgacagcaa cagtagtaaa agtcgcgtgt tataccaggt
     2281 ggctggcatt gccggtattc actctcagaa gattggcaat gctttacgta ccattgatac
     2341 ctggcatcca aaggttgatg aattgggggc tatagccgtg gaaccttacg gttctgtaac
     2401 cagtcgcggt gtggcctgcc gtcaaccgaa agaaaaactc gacttttata ccttactgga
     2461 caattgggtg actaaaggga tgaaaccaga cgttgagcag cagcactacg tgatggctgt
     2521 gctgatccgt ggcggtgtct ttggtgagaa atcggaataa ggagctgcca atggatcact
     2581 accttgagat ccgcgttctg ccagatcctg aattttcgag cgaaatgttg atggcggcgt
     2641 tatttgcaaa actgcatcgg gtattaggtg caagagggca aggcgatatc ggcgtgagtt
     2701 tccctgacgt aaatgttatg cctggagcgc gtttacgctt gcatggtagc gcccaggcat
     2761 tgcaggcgct tgaagcctca acgtggcgta aaggactaac ggactattgc caatgttcac
     2821 ctgtcactcc cgttccagaa ataaaaggct ggcgagtagt aagccgtgtg caggttaaaa
     2881 gtaaccctca gcgtctgtta aggcgttcag tgaaaaaagg ctggctaact gaagaacagg
     2941 caattgaacg actggcgacg caggctgaac agcggactga tttacctttt ctcaatatga
     3001 agagtttgtc gagtcagcaa ctgttcaagt tgtttatccg tcatggtgat ttgctcaaag
     3061 aaccggttaa aggggaattt agtagttatg gattaagcgc aaccgccact attccctggt
     3121 tttgatgctt tgtaattttt aacaggcctg gccatgtggt tgggcctttt tatgcgcgcc
     3181 atgcccaccg catacaaaaa aagcccgtac tttcgtacga gctcttcttt aaagatggcg
     3241 gtgaggcggg gattcgaacc ccggatacgt tgccgtatac acactttcca ggcgtgctcc
     3301 ttcagccact cggacacctc accaaattgt tttgctgcca gacctcatag gtggcaacgg
     3361 ggcgctacta tagggagttg gagtgaaacg gtcaagaaga atttatatag attgatttgt
     3421 ttggttacgc aatgaacacg ctgttcgcgg gacggagatt atgaccgtat gtgttctggt
     3481 caattgttta tcaaaagcta tgcagaaaat atgagattga agaaatacca aaccgaccct
     3541 ttttctaggt tgtaatgtaa ctcattgatt ttcttattgc tattttgaag tctggaaaaa
     3601 gggtttgaat ctgcgatttt gtaagtttta acagtaaatc aatcggatag tctgctatta
     3661 ttccagtcca ctgccgtaca ggcagcttag aaattaaaat aaatgcaacg gacaaagaag
     3721 ccattgttca ctgccgtaca ggcagcttag aaatggattc caaaccgccc accaacaaaa
     3781 acaggtgttc actgccgtac aggcagctta gaaagatacg atcgaattta gtcactgaat
     3841 acccccgttc actgccgtac aggcagtctt caaactcaac actccatcct ctaatattta
     3901 ttccccataa ctcatagacg caaaaaaggc cggttaaacc gaccttttac tcattctttc
     3961 tcttcgccca tcaggcggta aaacaatcag cgactacgga agacaatgcg gcctttgctc
     4021 aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg tcaggatgcg gatgtagttt
     4081 ttgcgcattt taccggagat gtgtgcagta accacgtgac cgttttctaa ctctacgcgg
     4141 aacatggtat taggcaacgt ttcaagaacg gtaccttgca tttcaatatt gtcttctttg
     4201 gccatctaat cctctggggt atcactaccg taatttgaac cggcaagata atgccgaagt
     4261 tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa acaggttcgg cacattactc
     4321 cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat aagggagcgg tgagataaac
     4381 gatgggcgtt acctgacgcg aaaaattcct tatcggcagc ggggtaatga gcgtaaccaa
     4441 ctctgcgacc gcaattataa cactctgggg agaaatgtgc cgaaaacatt cattcttgtg
     4501 gtgaaaacaa gcatcgaggt acccagaaat tattcggcaa tcgtccgagg cgcatttgat
     4561 tgagataatt aaggtaatcc cggcggggaa tttcgcaggc accaagcgat gctgtgtgat
     4621 cgttaaggac ctggcagtcg ataagtttac cgccatgacg gataaattca tcacagaaga
     4681 ccagaagcgc cgttttggac gcattttcca tccggctgaa catggactcg ccacaaaata
     4741 gtgttccctg ggccacgccg tacataccgc cgacaagctc atcttcacgc cagacttcaa
     4801 tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc ttcgaccacg ccacgcgtga
     4861 tccaggttcc ttcttcgcga tcgctggcac agccttcgat gacctgacca aaagcgtaat
     4921 tcatcgtgac acgatagggt gagcgtttat gaaatcgctt catactacgg ctgatatgca
     4981 gtgattctgg ccataacacc gcgcggggat cgggcgacca ccagaggatg gggtcgccag
     5041 gagaaaacca cgggaaaata ccacgctgat aagccattaa taggcgcgca gggctaagat
     5101 cgccccccag tgccagcagg ccgttaggct cacgtaatgc gccttccggg gaagggaagg
     5161 ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc aaaactccac gcaagtcgga
     5221 tcgttcaata atagcttaca aaccctgctt aaactggtaa taacgcccct gtctggcaag
     5281 cagttctgcg tgagtacctt gctcaataat ttgcccgttg tccatcacta ttatttgttg
     5341 gaaacgagag agtccgcgaa gtcgatgggt gaccattaac accgttttct cgcacatcat
     5401 ttctgcaagc aattcaagga tctgactttc ggttgtggca tctaagcctt ctgtaggttc
     5461 atccagcaat accagcggcg catcatgtaa cagcgcacgg gcgatagcca gacggcgcag
     5521 ttcaccaccg gagagctggc gtccgccttc acctaaccaa ctgttgagac ctgcatcctc
     5581 gagcagcttc tccagaccaa cgcgacgcaa gatctccgcc agagcctcat cactactacc
     5641 aggcgaggcg agtaacagat tatcacgcag cgtggcgcta aacagatgca ctcgctgggg
     5701 aacaacgctg atggtctgtc gtagagccgc ttcattcagg ctggctatgg gactatcgtt
     5761 aagcaaaatc tcgccctgtt gcgggtccca tgcgcgggtc agcagttgta acagtgttga
     5821 tttgccgcat ccggttcgcc cgagaatcgc aatatgttcc ccggcgttta cctgaagata
     5881 aatcccttta agtgcctgtt gggattgctc cggataagtg aactgaacat cccgtaatgt
     5941 cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg aaggtgactt ccggtttttg
     6001 atccgttaaa tcagtgatcc gtacggcaga ggcaatgact tgccccagat gctgaaatgc
     6061 acccgttact ggtgccagcg cttcaaacgc ggctaacgcg cagaagacaa acagggcaat
     6121 taacgcgccg ggttgagcat tgccgccaac gccaccggac gccatccaca gcatcaggat
     6181 caccgctaac gcgccaatga gcagcattat cgcttgcgac aatgcggtca gttcagattg
     6241 acggcgttgc gcttccagcc actgaatttc tgtattctct aattgcgtgc gataacgatc
     6301 gctggcacca aaaatggtca gctcagcttg cccttgcagc caggccgtca gttgttggcg
     6361 atactgcccg cgaagatgag tcagattttg cccggtgctt tttcccgcac gataaaacag
     6421 tggtggcatc aggaaaagcg tcagtaacat aatgccgccc agcgtaaagg cgagggtgaa
     6481 atcaaggaaa cttaacccga ttgtcaccac cataatcacc acaaaagcac ctaccagcgg
     6541 cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca tccgccacca cgcggttgag
     6601 taattcaccc tgacgatagc gcgccagtcc ggcaggggag aggggcagca atttgctgaa
     6661 ggtgtatatg cgcagatgct gcaacacgcg gaaagtcgcg tcgtgactta ccagacgttc
     6721 aaaataacgc ccggcagtac gggtgattgc tgcgccacgc acgcccgcag cgggtagcat
     6781 atagttgaag ctgtacagtc cggcaacccc cgctaccgct gaggcggaga ggaaccagcc
     6841 ggaaagggtc aacagaccga tactggcgag cagcgtgaca attgccagca cgataccaag
     6901 acttaacatc catttatgac gtttatacag tgccagatag ggtagcaaag cgcgcattta
     6961 aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg ccgccagcca cacttaattc
     7021 cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt acccaaatga catcccagtc
     7081 agcaagatct tctaactggt gggtgaccat taacgttgtc tggcgcagag aggcggtatt
     7141 cagcgcctcc attacgcgct gttcactgtg agcatcaagg ctggcagcgg gttcatccaa
     7201 cagtaatagc gaacagggat ttagtaacgc acgggccacc gccacgcgct gcgcctgccc
     7261 cacggaaagg cgggcagcct ggtcaccaac aggcgtatca atgccttgcg gcaggagcgg
     7321 tagaaactca ctgacccagg cgttatccag cgctgtttgt aactcttgtt cgctggcatc
     7381 aggtcgcgcc agtagtacgt tatcccgtaa tgttgctgcc ggtaattgtg ggttttgccc
     7441 aacccaggag agatgtttac gccatgattc cggtgataaa tcgcgtaatt ctatcccgtt
     7501 gattcgtagc gatccctgat atgagagaaa accagaaagc gcgttcaaca gtgaactttt
     7561 acctgaaccg ctgcgaccaa ccaacactgc tcgttggcct gctggcaaag taaaattcag
     7621 cggtccggcc agcgttttac cttccggcga cgtgataaac agatcctcgg cttcaatggt
     7681 caccggatcg gtcgatgcta attccgcctc accgcgctgc ggatgggcga gcggggtttc
     7741 cataaacgtt ttcaaactgt cagctgcacc aacagcctgg gctttagcat gataaaacgt
     7801 accgagatcg cgtaatggct ggaaaaactc tggcgcaagg atcagggcca gaaaacccgc
     7861 agccagcgtc acgccagtat cgtagtgacc aaaatccagc tcgccgaggt aggaaaaacc
     7921 aaagtagacc gccaccagag caatcgacag cgaggtaaaa aattcgagaa tgccggagga
     7981 taaaaacgcc agccgtagca cttccattgt ccgttggcgg aaatcttccg aagcagaacg
     8041 aatactttca atttcagctt caccacgacc aaaaatacgc aatgtttcca tgccgcgcag
     8101 gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga aagttacgtc ggttagcatc
     8161 ggcagccccc attccaacca gcgccataaa taacggaatt agcggtgcag tgcccagcag
     8221 aatgagcgcc gcggcccagt tagaggggaa gatagccacc acaatcagca gcggcaccga
     8281 cactgccagc gccatttgcg gcaggtagcg tgcatagtaa tcatgcatat cgtcaatttg
     8341 ctcgagtacc agcgtcgccc agctccccgc tggtttaccc tgaatccacg ctggccctgc
     8401 ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag cggatatgct gcccggcgtg
     8461 ataacccacc cgttcgcgta accagaccac ccatgcgcgc agtacaaagg tcagaaacag
     8521 taacgtaaag ggaagcagca gggcttcacg gggaatattc tccataatca tatgttgcag
     8581 aattcgcgcc atgaaccagg cctgggaaat gatcaatatg ccgctcacaa agcccagcag
     8641 acgagaaata ttcagccaac gttgggagat gacgctttgc tgttttaacc agcgggttaa
     8701 ttctttttga cgggatttat tcattgcacg cttagcaggt gagttatcag aattatttgc
     8761 agagcaatgt tacaacgggg aaaaaataaa ggcgacccat agtcgcatgg tgtcgccttc
     8821 tttacttttg ttactgattt gtaaaattat tttgcgtcag ctaaaccgtc gaggtagcgt
     8881 tccgcatcaa gagctgccat gcagcctgta ccagcagatg taatggcctg gcgataaatg
     8941 tgatccatca cgtcgcctgc ggcaaagacg ccagggatgc tggtctgggt ggcattacca
     9001 tgaatacccg actgtacttt gatgtagccg ttttccagtt ccagctgccc ttcgaaaatg
     9061 gcagtattcg ggctgtgacc gatagcaaca aacagaccgg caacgtcgag tgactcgatg
     9121 ttatcgctgt tttgcgtatc gcgcagacga acgccagtga cgcccatctg atcgccggtc
     9181 acttcttcca gcgtacggtt gatgtgcaga ataatgttgc cgttctccac tttatccatc
     9241 agacgcttaa tgaggatttt ttccgcacgg aaaccatctc ggcggtgaat cagatgaact
     9301 tccgaagcga tgttagacag atacagcgcc tcttcaaccg cggtattgcc gccgccgatg
     9361 accgcaactt tctggttgcg atagaagaaa ccgtcgcagg ttgcacaagc agaaactcca
     9421 cggcctttaa aggcttcttc agagggcagg ccgagatagc gagcagaagc tccggtggca
     9481 ataatcagcg cgtcgcaagt gtattcgccg ctatcgccag tcagacggaa cggacgattc
     9541 tgcaaatcaa ctttgttgat gtgatcaaag atgatttcag tttcaaattt ggtggcatgt
     9601 tcgtgcatgc gctccattaa taacggaccg gtcagatcgt ttggatcgcc aggccagttt
     9661 tccacttccg tggtggtggt cagttggccg cctttttcca tgccggtaat cagcacaggt
     9721 tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc ccgccgggcc tgaacccagg
     9781 ataagcagtt tactgtgttt ggtcgtgccc atgagatccc catagttgtt ggcagacaat
     9841 ggcaggattg tagggaattt acagacgtaa aaaaagagta tgacgatttt gttaacaatt
     9901 tgtgcaatcg gcagcatcga taagcaggtc aaattctcac gtcattatca cctctgctac
     9961 ttaaatttcc cgctttataa gccgattaaa tgatgaataa acgcccctgt taatgaatat
    10021 ctggcatgtt gtactaaaaa tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa
    10081 acattcgagg aagaaaaaaa aacagtattc ttatatgcgc ataaccatgc atgtaaatac
    10141 catgtttacc gtgctagtga aatctacgta tggcgtggac agacgccatt cgtgatgtcg
    10201 atagctgcca caaggcaacg gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc
    10261 ttcatgattt cggtctatcg tgacgggtag cgactctgaa cagtgatgtt tcagggtcag
    10321 acaggagtag ggaaggaata cagagagaca ataataatgg tagatagcaa gaagcgccct
    10381 ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt
    10441 atttctaacg tcgagctttc taaacgtgtg ggactttccc caacgccgtg ccttgagcgt
    10501 gtgcgtcggc tggaaagaca agggtttatt cagggctata cggcgctgct gaacccccat
    10561 tatctggatg catcacttct ggtattcgtt gagattactc tgaatcgtgg cgcaccggat
    10621 gtgtttgaac aattcaatac cgctgtacaa aaacttgaag aaattcagga gtgtcattta
    10681 gtatccggtg atttcgacta cctgttgaaa acacgcgtgc cggatatgtc tgcttaccgt
    10741 aagttactgg gggaaaccct gctgcgtctg cctggcgtca atgacacacg gacatacgtt
    10801 gttatggaag aagtcaagca gagtaatcgt ctggttatta agacgcgcta acacggaaca
    10861 ggtgcaaaat cggcgtattt tgattacact cctgttaatc catacagcaa cagtactggg
    10921 gtaacctggt actgttgtcc gttttagcat cgggcaggaa aagcctgtaa cctggagagc
    10981 ctttcttgag ccaggaatac actgaagaca aagaagtcac attgacaaag ttaagtagcg
    11041 gccgccgtct tctggaggcg ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg
    11101 ctgccttact aagctttaac ccttcggacc ccagctggtc gcaaacggcc tggcatgaac
    11161 ctatccataa tttaggtggg atgcccggtg cgtggttggc agatacgctg ttctttattt
    11221 ttggcgtgat ggcttacacc attcccgtca ttattgtcgg cggttgttgg tttgcctggc
    11281 gtcatcagtc cagcgacgaa tacattgatt attttgccgt ttcgctacgc atcattggcg
    11341 ttttggcgct catccttacc tcctgtggtc tggcggcaat caacgctgac gatatctggt
    11401 attttgcctc cggtggcgtc attggcagct tactaagcac tacgctacaa ccactgctac
    11461 acagtagcgg gggaactatt gcgctgctct gcgtttgggc agcgggcctg acgctgttca
    11521 ccggttggtc atgggtgacc attgctgaaa aactcggcgg ctggatttta aatattctta
    11581 ccttcgccag taatcgtacc cgtcgcgatg atacctgggt cgatgaagat gagtatgaag
    11641 acgacgaaga gtatgaagat gaaaatcacg gcaaacagca tgaatcacgc cgtgcccgta
    11701 ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg
    11761 ggcggcaaac agatgctgcg ctgttctccg gtaagcggat ggatgatgaa gaagagatta
    11821 cctacactgc acgcggtgtg gctgccgacc ctgacgacgt cctgttttcg ggcaatcgtg
    11881 caacgcagcc agaatatgac gaatacgatc cattattaaa cggtgcgcca attaccgaac
    11941 ctgttgctgt agcagctgct gctaccacgg cgacacaaag ctgggctgca ccggttgaac
    12001 ctgtgactca gacgccgcca gttgcctctg ttgatgttcc acctacgcaa cctacagtag
    12061 cctggcagcc tgtaccgggg ccgcaaacgg gagaaccggt tattgcacct gcaccggaag
    12121 gttacccaca gcagtcacaa tatgcgcagc ctgcagtgca atataatgag ccgctgcaac
    12181 aacctgtaca gccacagcag ccgtattatg cgcctgcggc tgaacaacct gtgcaacaac
    12241 cttactacgc tcctgcggcg gaacaacctg ttcagcaacc gtattacgcc cctgcgccag
    12301 aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccact tttgctccac
    12361 agtctacata ccagacggag caaacttatc agcagccagc tgctcaggag ccgttgtacc
    12421 aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga
    12481 caaaacccac gcgtccgccg ctttactatt ttgaagaagt ggaagagaag cgagcccgtg
    12541 aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa gaaccggaac
    12601 cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta gaagccgctg
    12661 ccgctgtttc tccgctggca tctggtgtga aaaaagcgac actggcgacg ggggctgccg
    12721 caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt ccgcaggtca
    12781 aagaggggat tggcccgcag ttgccacgac cgaaacgcat ccgcgtgcca actcgtcgtg
    12841 aactggcgtc ttacggtatt aagctgccct cacagcgtgc ggcagaagaa aaagcccgcg
    12901 aagcccagcg taatcagtac gattctggcg atcagtacaa cgatgatgaa atcgatgcga
    12961 tgcagcagga tgaactggca cgtcagttcg cccagacgca gcagcaacgc tatggcgaac
    13021 agtatcaaca tgatgtgccc gtgaacacag aagatgcaga tgctgcggca gaggcagaac
    13081 tggctcgtca gtttgctcaa actcaacaac aacgttattc cggcgaacaa ccggctgggg
    13141 cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg
    13201 gtccacacga accgctgttt acgccaattg ttgaacctgt
//
LOCUS       DADTFG010000136        13152 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.137, whole genome shotgun sequence.
ACCESSION   DADTFG010000136 DADTFG010000000
VERSION     DADTFG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.137"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            135..1634
                     /gene="pitB"
                     /locus_tag="J1138_003675"
     CDS             135..1634
                     /gene="pitB"
                     /locus_tag="J1138_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBA8455423.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLNGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMQCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1683..2375)
                     /locus_tag="J1138_003676"
     CDS             complement(1683..2375)
                     /locus_tag="J1138_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBA8455424.1"
                     /translation="MQSIIPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            2550..3263
                     /locus_tag="J1138_003677"
     CDS             2550..3263
                     /locus_tag="J1138_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBA8455425.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELASRMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKNQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3295..4053
                     /locus_tag="J1138_003678"
     CDS             3295..4053
                     /locus_tag="J1138_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455426.1"
                     /translation="MDALQTQTVKSTTAPQPNYIPGLIAVVGCDGTGKSTLTNDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKIRMF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4099..5448
                     /locus_tag="J1138_003679"
     CDS             4099..5448
                     /locus_tag="J1138_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBA8455427.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNSTGEKS"
     gene            5448..6284
                     /locus_tag="J1138_003680"
     CDS             5448..6284
                     /locus_tag="J1138_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455428.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGHFQDGFRNGKKNIVSFYGNLLRVYLSSPVWWLVNYSLRHSQ
                     LETVNGFIAYRQKQMYILKEIIAKPQLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALLIFRFLAAINGLMGDKHDDMVADDMDNRRSYDAPVALDSDIRQRLELLISRFPLYP
                     EQ"
     gene            6284..6844
                     /locus_tag="J1138_003681"
     CDS             6284..6844
                     /locus_tag="J1138_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBA8455429.1"
                     /translation="MPSRWIFALIIALLAPGAAAHNFVTGKTVTPVYIQEGGELLLNR
                     DDEIHYQKWNSTQLTGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFSQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWQLPEQSSTILVLNKAGRI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6875..7945
                     /gene="lptF"
                     /locus_tag="J1138_003682"
     CDS             6875..7945
                     /gene="lptF"
                     /locus_tag="J1138_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBA8455430.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYASVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7942..9021
                     /gene="lptG"
                     /locus_tag="J1138_003683"
     CDS             7942..9021
                     /gene="lptG"
                     /locus_tag="J1138_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBA8455431.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(9056..10228)
                     /locus_tag="J1138_003684"
     CDS             complement(9056..10228)
                     /locus_tag="J1138_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBA8455432.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10228..10476)
                     /locus_tag="J1138_003685"
     CDS             complement(10228..10476)
                     /locus_tag="J1138_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA8455433.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENN"
     gene            complement(10508..11422)
                     /locus_tag="J1138_003686"
     CDS             complement(10508..11422)
                     /locus_tag="J1138_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBA8455434.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPQPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11419..13143)
                     /locus_tag="J1138_003687"
     CDS             complement(11419..13143)
                     /locus_tag="J1138_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBA8455435.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVVLQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
BASE COUNT         3156 a         3254 c         3401 g         3341 t
ORIGIN      
        1 atgaaggctt attgtctgat acacaagaaa taacacgctt tttatcgtta aaaaatgata
       61 tttcactttg cccacgccgt gaaagtcact gataatgcct ccgttcgtaa attcaaaatg
      121 gcgtaatcta atatatgcta aatttatttg ttggccttga tatatatacg gggcttttgt
      181 tattacttgc tctggcattt gtgttgttct atgaagcaat caatggtttt catgacacgg
      241 cgaatgcggt ggcaaccgtt atttataccc gtgccatgca accacagctt gctgtggtca
      301 tggcggcatt ttttaacttt tttggcgtat tgcttggcgg gcttagcgtt gcctatgcca
      361 tcgtccatat gttgccaacc gatttgttgc tgaatatggg gtcaactcac ggtctggcga
      421 tggtcttttc catgctgctg gcggcgatta tctggaatct gggaacgtgg ttctttggtt
      481 taccggcctc cagttcgcac actttgattg gtgcgattat cggcattggt ttaaccaacg
      541 cgctgttaaa tggttcgtcg gtgatggatg cgttaaacct gcgcgaagtc accaagattt
      601 tctcttcact cattgtctct cccatcgtgg ggctggtgat tgcggggggg ctaatttttc
      661 tgctacgccg tttctggagt gggacgaaaa agcgtgaccg tattcatcgt attcctgaag
      721 atcgcaaaaa gaaaaaaggc aaacgtaagc cgccattctg gacgcgtatt gccctcattg
      781 tttccgctgc gggcgtggcg ttttcacatg gcgcgaatga cgggcagaaa ggaattggtc
      841 tggtgatgct ggtgctggtg gggattgctc ccgccgggtt tgtcgtcaat atgaacgcgt
      901 ccggctatga aattacccgt acccgcgatg ccgtcaccaa cttcgaacac tacctgcaac
      961 agcatcctga actgccgcag aagttgatta cgatggaacc tccattgcct gcgacatcga
     1021 ctgatggcac gcaagtaaca gagtttcact gtcatccggc aaataccttt gatgcgattg
     1081 cgcgcgttaa aacgatgctg ccaggcaata tggaaagtta cgagccgtta agcgtgagtc
     1141 agcgcagcca gctgcgccgc attatgcagt gcatctctga tacctccgcg aagctggcga
     1201 aactgccagg cgtcagtaaa gaagaccaga acctgctgaa aaaactgcgc agcgatatgt
     1261 taagcaccat tgagtacgca ccggtatgga tcatcatggc ggtagcactg gcgctcggca
     1321 tcggcaccat gattggctgg cgtcgtgtag cgatgaccat cggtgagaag attggtaagc
     1381 gcggcatgac gtatgcgcaa ggcatggcgg cacaaatgac ggcggcagtg tctatcggcc
     1441 ttgccagtta tattgggatg cccgtctcca caacacacgt actctcgtct gcagtagcag
     1501 ggacgatggt ggtggacggc ggtgggttac agcgtaaaac ggtaaccagc atcctgatgg
     1561 cgtgggtgtt tactttaccg gcggcgattt ttctttctgg tgggttgtac tggatagcat
     1621 tgcagttgat ttaaccttcc tgaaaatgcc cggtcctggc gatcgggcat ttccattttt
     1681 cactagtgat aaccacgcgc ggtcataaaa tccgtaatcg ctttttctgc atcaaccagc
     1741 accttttcca gtggctgatt ggcgtcgata tcaaccagtt gtgcaccacc aaaggttaac
     1801 tgtggcgtta tggcaatctt cctcgccagc gattcccgtt tatggtcggg tttacgcgcg
     1861 caggcaactt caaggtcaac attaagtttg atgaccagat cgggcttgtg gctcgccatc
     1921 cagtgaaatg ctttacgttc ctgacttgcc agccatgaga caaaacgacc accttcaacg
     1981 ttaggcggaa acaccgtacc atcataagcg ccaggaatct ggtcctgcgg ataacggtcg
     2041 gttaaaacaa ttaacccgcg acgacgacag gcaagcatat gacgaaagcg cagtaagcgg
     2101 cgggcgacaa acgctgtaat taccagcgcc ggaactggtc caggcaattt ttttgctgtt
     2161 tttacctgat ttcgttcaat tgttttatgt aaggattttc ccatcaacgg taattttgtc
     2221 actgcacgac cgacatttcc ggcctgtttt cctaaatgaa ccctttcggc agcaccatat
     2281 ttttcgacaa cggtaataag atgttcacac accgttgact tgcctgaacc atcgctacca
     2341 ataacggcaa ttaatggagg tattattgac tgcatgaata tatccttaaa tttaaatatc
     2401 catccaaaat atttatttgg ttaatatgtt tttgatgaaa gcgtaattca ggtcaatgtc
     2461 acaattaacc attgtcacaa taaggttgaa cggatattct gagcacggac cacgtaatat
     2521 tcaatacatt atttgctgcc gggtttttcg tgaatcaacg taatatgtca ataattaatt
     2581 ccacgccagt gcgtgttatt gccattgtag gatgtgatgg ttcaggtaaa tcgaccctca
     2641 cggcaagcct ggtaaatgaa ctggcatcaa gaatgccaac agaacacatt tatctcgggc
     2701 aatcgtccgg gcgaattggc gaatggattt cacagctacc tgttattggc gcaccttttg
     2761 ggcgttattt gcgaagtaaa gcggcacatg tgcacgaaaa gccctcaaca ccgcctggca
     2821 atattactgc gctggttatc tatctgcttt cctgctggcg ggcgtacaag tttcgcaaaa
     2881 tgttgtgtaa aaaccagcaa ggctatctgc tcatcaccga ccgctacccg caggttgaag
     2941 tgccgggttt ccgctttgat ggcccgcaat tggcaaaaac cacgggcggt aacggttgga
     3001 taaaaatgtt aaggcagcgt gagctgaagc tgtaccaatg gatggcgtct tatttgcctg
     3061 tattgttgat tcgtcttggc attgatgaac aaaccgcgtt tgcgcgtaaa cctgaccacc
     3121 aactggcagc gttacaggaa aaaatcgccg ttacgccgca actgacattc aatggcgcca
     3181 ggatccttga actggatggt cggcaaccag ccgatgaaat tctgcaagcg tcactacgcg
     3241 caattcacgc cgcgctttcc tgatcaccaa aatggatagg ggatgattaa acgaatggat
     3301 gcactacaaa cacaaactgt taagagtaca accgcgccgc agcccaacta cattccgggg
     3361 ctgattgcgg tggtcgggtg tgatggcacc ggtaaatcca cactgaccaa tgacctggtg
     3421 aaatcgctgc aacaacactg gcaaaccgag cggcgctatc tggggctgct ctccggcgaa
     3481 gacggcgaca aaatcaaacg attgccgttg gttggtgtct ggctcgaacg gcgactggcg
     3541 gccaaatcct cgaaaaccca aagcatgaaa accaaatctc cggcgctatg ggcggcggtg
     3601 attatgtact gcttctcgct gcgaagaatg gcgaatctgc gcaaggttca gcgactggcg
     3661 caaagtggcg ttctggtggt cagcgatcgc ttcccgcagg ctgaaatttc gggcttttat
     3721 tatgacggac cggggattgg cgtcgaacgt gcgaccggga aaatcagaat gtttctggcg
     3781 cagcgtgaac ggcgtttata ccaaaaaatg gcgcaatacc gtcctgaatt aatcattcgc
     3841 ttggggattg atattgatac tgccatctcc cgcaagcctg accatgacta tgccgagctg
     3901 caggacaaaa tcggcgtcat gtcgacgatt ggctataacg gcacaaaaat tcttgagatt
     3961 gattccagag cgccttacag cgaggtgctg gaacaggcac agaaggcggt ttctctggtt
     4021 gccatcgttt ctgaccgccg aagtttaact taaggctggc gggatacttt ttactatcaa
     4081 cgataaggtc aggctgaatt ggcgggtttt aacatcaaac attggtttgc agatggcgcg
     4141 tttcgcacca ttattcgcaa tagcgcctgg ttaggctcca gtaatgttgt gagcgctttg
     4201 ctggggctgt tggcgctctc gtgtgccggt aaagggatga cgcccgccat gtttggcgta
     4261 ctggtaattg tgcaatcgta cgccaaatcg atcagcgatt ttattaagtt tcagacatgg
     4321 caactggtgg ttcagtacgg aacgccggcc ttgaccaaca ataatccgca gcaatttcgc
     4381 aatgttgtct cattttcctt ctcgctggat atcgtcagcg gcgcggtggc gattgtcggc
     4441 ggcattgcct tactgccatt cctttcccat tcattaggtc tggatgacca aagcttttgg
     4501 ctggcggcgc tctattgcac gctcattcct tcaatggctt cctccacgcc gaccggcatt
     4561 ctgcgtgcgg tagatcgctt tgatttgatt gctgtacagc aggcgacgaa accttttctg
     4621 cgcgcagcgg ggagcgtcgt agcctggtat tttgactttg gttttgcggg ttttgttatt
     4681 gcctggtacg tgtcgaatct ggttggcggc accatgtact ggtggtttgc cgcgcgcgaa
     4741 ttacgccgca gaaatatcca taacgccttc aaattgaatc tgtttgagtc tgcccgacac
     4801 attaaaggcg cgtggagttt tgtctggtca accaacattg cccactccat ctggtcggcg
     4861 cgtaactcgt gcagcacagt gttggtgggg atcgtgttag gacccgctgc cgccgggtta
     4921 tttaaaatcg ccatgacatt cttcgatgcc gccggaacgc cagcgggttt actgggcaaa
     4981 agtttttacc cagaggtgat gcgtttagat ccacgcacca ccagaccgtg gttgttaggc
     5041 gtgaagtccg gtttactggc gggcggaatc ggtattctgg tggcgctcgc ggtgttgatc
     5101 gtcggcaagc cgctcatttc gctggtgttt ggcgttaagt atctcgaagc gtatgacctg
     5161 attcaggtga tgctgggcgc aattgtgatt tcgatgctgg gcttcccaca ggagtcattg
     5221 ctattgatgg cgggaaaaca gcgcgctttt ctcgtggcgc aaaccatcgc ctcaatcggc
     5281 tacatcgtat tgctgttcat gttctgtcat ctgtttggcg tgctgggggc tgcatttgct
     5341 tacttcggtg ggcaatgtct ggatgtggtg ctctcgctaa ttccgacctt aaaggcattt
     5401 tttcagcgcc attccttgct ttataactca accggagaga aatcctgatg atgagaaaat
     5461 atttcccgct tgaggcgagc gagcgtctat ttgttgcgat tgaagaagat gatgttgttg
     5521 atgcacaggt cagtttgccg ccgactatcg cactaagttg taccaccgaa atcatccatg
     5581 ataattacgc gttatgcctg aaattctggc tggatggtgt taatcgtcag gagttactgc
     5641 gtttaatccg caaacaggcg aaaggcgatg agctgacgac agacgagcgc aaacagttta
     5701 aatacatgcg agcgcgctac aagcatctgc gttttgcgca acggctgtat ttaaagaagc
     5761 atcaggctgg atttttattc ggcaaaacga ccgtttttct tgggcatttt caggatggtt
     5821 ttcgtaacgg taagaaaaat attgtgtcgt tttacggtaa cctgttacgt gtttatttga
     5881 gctcccctgt ctggtggctg gtgaactatt cattgcgcca tagccagtta gaaaccgtga
     5941 acggttttat cgcctaccgc cagaagcaaa tgtacatcct gaaagagatt attgctaaac
     6001 cgcaactaac tggcagggaa tttcatgatg tgcgaaaaat catcagccag caggtttctt
     6061 attacgacac cttaagatcg cttgatcctg aaaataaaga ggcgttactg attttccgtt
     6121 ttctcgccgc cattaatggt ctgatggggg ataagcacga cgacatggtg gcggatgata
     6181 tggataaccg tcggtcatat gatgcaccgg tggcgctgga tagcgatatt cgccagcgtt
     6241 tagaactgct tatttcgcgt ttcccactct atccggagca ataatgccat ctcgttggat
     6301 tttcgctctg atcatcgcac tcttagcgcc aggcgcggcg gcacacaatt tcgtcactgg
     6361 caaaacggtt actcctgttt atatccagga aggcggcgag ctgcttctga atcgcgacga
     6421 cgaaattcac taccaaaagt ggaacagcac acaattgacg ggaaaagtcc gcattattca
     6481 gtatatcgcc ggacgtaagt ctgcgaagaa aaagaactca ctgttaatta aagcggttga
     6541 agcggcaaac ttttcgcagg atcgttttca gcccaccact attgtgaaca ccgatgatgc
     6601 catctttggt actggctatt ttgtcgttgg caaaatcgaa aaaaataaac gccgctatcc
     6661 gtgggcgcaa ttcgttattg acggcaatgg tctgggacgc gttgcctggc aattgccgga
     6721 gcagagttca accattctgg tattgaataa agccgggcga attcagtggg caaaggacgg
     6781 cagcctgaca ccggaagagg tagatcatgt cattgcgctg gcgcagaaac tgatcaatga
     6841 ataaaaaatt caaaggattg agggcgttag cgagatgaag ctggttgaac actacatcat
     6901 gcgtggtacg cgccgtctgg tgctgattat cgtcggtttt ctgatcttta ttttcgccag
     6961 ctactccgca cagcgttatc tgaccgaagc ggcaaatggg acattagctc ttgatgttgt
     7021 gctggatatc gttttctaca aagtgctgat tgcactggag atgctgttac ctgttggact
     7081 atatgcttca gttggcgtga cgctagggca aatgtatacc gactcggaaa ttaccgctat
     7141 ctccgcggcg gggggcagtc cgggacgttt gtacaaagcc gttctttatc tggcgatacc
     7201 gttaagtatt tttgtcaccc ttctgtcgat gtatggtcgt ccctgggctt atgcgcagat
     7261 ttatcaactg gagcaacaat cgcagtcgga gctggatgtt cgtcagttgc gggcaaagaa
     7321 atttaacact aacgataacg gacgaatgat cctttcgcag acggttgatc aggataataa
     7381 tcgcctgact gacgcgctga tttatacttc tactgccaat cgaacccgca ttttccgcgc
     7441 ccgttcggtt gatgtggttg acccatcacc cgagaaaccg accgttatgt tgcataacgg
     7501 gaccgcctat cttctcgatc atcaggggcg tgacgacaac gaacagatct accgtaatct
     7561 gcaattacat ctgaatccgc tggatcaaag ccctaacgtc aaacgcaaag caaaatcggt
     7621 cacggagctg gcgcgctccg cctttcctgc cgatcatgcc gaactgcaat ggcgacaaag
     7681 ccgtggcctg accgcattgt tgatggcgct gctggccatt tcattaagtc gggtaaaacc
     7741 gcgacagggg cgattttcaa cattattgcc actgacgttg ctgtttattg ccatttttta
     7801 tggcggcgac gtctgccgta cgctggtggc taacggcgcg attcccctca ttcctggttt
     7861 gtggttagta cccggactca tgctaatggg cctgctgatg ctggtcgcac gcgacttctc
     7921 tttgctgcag aaattttccc gatgaatgtt ttcagtcgct atttaatccg tcatctcttt
     7981 ctcggttttg ccgccgccgc agggctattg ctgccgcttt ttaccacctt caacctgatt
     8041 aacgaactgg atgatgtcag cccgggcggt tatcgctgga ctcaggcggt gctggtggtg
     8101 ctaatgacct taccgcgcac actggtcgaa ctttcgccgt ttatcgcctt attgggaggg
     8161 attgttggcc tggggcagtt atcgaaaaac agtgagctta ccgccattcg cagcacgggg
     8221 ttttctatct tccgtattgc actggtggcg ctggttgccg ggatattgtg gactgtttcg
     8281 ttaggcgcga tagatgagtg ggtggcgtcg ccattgcagc aacaggcgct gcaaatcaaa
     8341 tcgaccgcca ccgcgttggg ggaggacgat gacattaccg gcaatatgct gtgggccagg
     8401 cgcggcaatg aatttgtgac ggtgaaatcg ctgaatgagc agggccagcc ggtgggcgtg
     8461 gagatatttc attatcgcga cgatctctcg cttgaatcct acatttatgc acgcagtgcc
     8521 accattgaag acgacaaaac gtgggtcctg catggtgtga atcataaaaa atggctgaat
     8581 ggcaaagaaa cgctggaaac attagataat cttgcctggc aatcggcctt caccagtatg
     8641 aatcttgaag agttatcaat gccggggaat actttttctg tccgtcagct taatcattac
     8701 atccattatt tgcaggaaac cgggcaaccc agcagcgaat accgccttgc actgtgggaa
     8761 aaactggggc aaccgatcct gaccctggcg atgattttgc tggctgtgcc gtttaccttt
     8821 agcgccccgc gctcgccagg gatgggtagc cgtctcgctg taggcgtcat cgttggctta
     8881 ctcacctgga tcagctatca aatcatggtc aatttgggat tgctatttgc gttaagcgca
     8941 cctgttaccg cgctcggttt accgatagcg tttgtgctgg tggcgttgag cctggtgtat
     9001 tggtatgaca gacaacatta aacccgccgt ggcgcaacac cacggcgggc tcacattact
     9061 gcttcagagt agcaaatgcc tgaatgatgg catcaatttg ctcacgactg tgggcagcgt
     9121 ttacgctaca gcgcagcagc gtaatgcctg caggcgctgc aggtggcagg ataagattca
     9181 cataaacgcc cagcgaaata agctcgcgcc agatgcgtaa accttcctct ttcgaaccga
     9241 ttattaccgg cactaccggg ctaatatgtg aacttaactc ataacccagt ttttgcagac
     9301 cgtcatacag atggttggcg ttatccatca gcttttgccg cagctctggt tgtgtggcaa
     9361 tagttcttaa cgacgagcgc acggtcgcaa tgcaggacgg agaaggagag gcggtaaaaa
     9421 tatacgggcg gctgccgtag cgcagcactt ccatcgcttc cgaaccgaca gcaaaaccgc
     9481 caatcgaggc cagactttta ctgaaagtac caacgataat atccacatca tcttcaacgc
     9541 cgacagcttc agccagcccg cgccccgttg cgcctaacac gccaaacgaa tgcgcttcat
     9601 cgacaatcaa ataaccaccg agacgacgtt tgatatcaac aatttccgcc agcggcgcaa
     9661 cgtcaccgag catactgtag atgccctcaa cgatgataat cgcctctttc gcccgttcgc
     9721 cgaggcgaac catgcgccgt tccagatctt tagcatcgtt atggcggaaa cgaataattt
     9781 ccgcgccacc gagagaacag gcgtcataaa tactggcgtg gctgtcggca tccagtaaca
     9841 ccacggcgtt atggtcggca agtgcgctga tgacgcctaa attagcggta taaccggttg
     9901 aaaaaacgat agcggtaggg cgattgaaaa actcggcaat ctctttctct aacgccaggt
     9961 gcggcgcata actgccgttt gccatacgag aaccggtggt gccggtgcct tgggccgcta
    10021 acgctgcctg accttcggcg atggcatcat gattaaaggt taaaccgagg taattattgg
    10081 tcccggccag aatcactttt ttattgccga tccgaccttc ggtggcggaa tacacttcat
    10141 caatacaggt gccaaacggt gttaacccgt tgtcactaaa ttgcagtcgc tcgccagcaa
    10201 ggcgcgcata tttatcgtat agccccatta gttattctcc agccatggaa gtagagtttc
    10261 cattaattgc gcaggggttt tgacatccag cagaatatta ataggaatgg aaatatcgaa
    10321 tctgtcttcc agcatcatta agagatccat cactttgatc gactccaggc caagatcgtt
    10381 gacgagatcg ctgtcgggct taatttccac accgttttcg accaaatcct gtaaacagga
    10441 aaggatgtaa tccattacta tttcacgatt taccatagaa acgttaacac acctttaaac
    10501 aagacaatca aaatagcccg ttgcgtaatg cgtgttccag gctaatcccg ggaaaccaat
    10561 taatatcttc agaaatacgg ttattacttg ccgaccagtc ggcatgggtt aattcacgaa
    10621 ttttcgagcg agtcagcatc ggttctttac ccgccagacg gctcaacgcg gtactgatat
    10681 ccgcaaggca ggtgagtagc ggctgaggaa taccgaccat ccgcacggaa ccacagcgga
    10741 catcggcaac aagctgttgg acacgttgcc agtcatagcc tccggcgacg ccatcgcata
    10801 attcataggt ttgcgtctgt acagtttcgg cgcttaacca ctgacccact gcttgcgcaa
    10861 aatcggtgac gtgaagaaaa gagagctgtg tctctggcgt gccaagtcgt ggcagcagac
    10921 cgcgcagcat ccagtcaaac agcggtttta actctttatc gccgggacca tacacggctg
    10981 tcgggcgaaa aacgcccaac gtaatttcgt cagccattgc agtcagccgt tgttcggcga
    11041 cgtgtttgga atttgcgtac caggagagtt cgggatggcg cgccgccagc gaagagataa
    11101 acagaaaacg ttggcaaaag ccgctctctt ttgccgcttg catcaggcgc aggctgccgt
    11161 caacgttgca gcgggtaaaa atctcttctt tgtgcccgcg cacttgcccg gcgcaatgga
    11221 ccaccgcgct ggctccggca accagttcgc taagcgaatg tgtatcttcc agcgaaccgc
    11281 gcacccaagt gagattatca ttgacgtgag cgcgggcagt acgcgtcaat gcgcgaacgt
    11341 gaaagccgcg ggcgagcagg ttatcaataa tatatttacc gataaacccg gtagcgcccg
    11401 tcaccgcgac agtttgattc atgccaggga ttcctgcaca tgaagactgg cagcataagc
    11461 cttctgataa cgttttttcg cttccgcacg ggcaggcttg ccggaggatg ttcggggaat
    11521 actgtggggc ggcaacagat cgatatccgc ggtaacgcca aattcgcttt ggatccgagc
    11581 tgccagcgcg tggataagct gcccgcgacg ttcttcgtcg ctgatccgac actggatctg
    11641 caaaatgatt ttttcctggg cggtaacaaa agcaatcgca tcgccagaat gaatttccgg
    11701 ctcctgttcc gcaatgtatt caatatcctg cggccagata ttacggccac gaataataat
    11761 cagatcttta atgcgtccgg tgacatacag ataaccgtcc agcagataac caaggtctcc
    11821 ggtgtctaac cagcccgtcg cggcaatctc gtcttgcgaa acctggtcgc caaagtaccc
    11881 gctcatcagg ctggggccgg agatgcaaat atggcctacc acacgttccg cgacaggtat
    11941 atctgcttca ttgcggattt caatgccatg ttccggcaac gctttaccgc agttgacgaa
    12001 agtcgatacg gcgcgtgttt cagcacccgg cgcgacggct ttaccctggt attcgaggat
    12061 gtcgcgatcc acttcgttaa ccacaacccc gcaggcttca tcagagaagc tgacagccag
    12121 cgcattttct gccagtccgt agcacggcat gaaagtttta tcgtcaaagt taacctgacg
    12181 gaaacattca gcgaattgat ggagttgttc tgcggatatg ggttctgcgc caataccagc
    12241 gacgcgccag caggaaagat ccagttcagc gagatctttt tcattcacgc ggcgctggca
    12301 caattcatag ccaaacggcg gtgcaacgga aacggtgcca cgatttttac tgatcaattt
    12361 aagccattgc agaggacgca tggcaaaatc ctgagtgcgc aaataatcta ctgaaagctg
    12421 cgtggcgacg ggggtcagga gaaagccgac cagtcccata tcatggtaga aaggcagcca
    12481 ggagacgcag cggtcgccag ggcgtaattt aatcccatcg tggcttatag cacgtagatt
    12541 agccatcact tcgtgatggg tgataatgac gccacgggga aaacgggtgc tgccggaggt
    12601 gtactggagg taggcgatat cgtttggaac tggacgctgg agcacaacat cggcttccgg
    12661 taatgcctta aaccaggcgt ggcttaaaac atgtaattcg gggttgttgt tatgcgtcgc
    12721 ggcattgacc agtggcaacc actcatcacc agtgataatg gctgcgggct ggcaacttgc
    12781 cagtaaaccc tgcagtttgg cgctccagga atcccgctga cctacgccca ttggaatcgc
    12841 caacgggacg gcgactaagc cggcatactg gcaggcaaaa aaagcctcta caaacccgct
    12901 actcgtttcg gcaatcagcg ccacgcgatc gccttttttc aggttcagcg ataacaacct
    12961 tttcgcacca gcttcggcac gcgcttttaa cgtctgatat tccagttgat cttcaagttg
    13021 gcaacgtctg tcataaaaat tcattccggc gctactcaga gcggcgtagt ccaaagcatc
    13081 aaccagcgtt ggaaaatcgg catagcgcat aggtagggaa tgcgtaaaga ttttattaga
    13141 catatacacc ac
//
LOCUS       DADTFG010000137        13138 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.138, whole genome shotgun sequence.
ACCESSION   DADTFG010000137 DADTFG010000000
VERSION     DADTFG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.138"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            188..1390
                     /gene="proV"
                     /locus_tag="J1138_003688"
     CDS             188..1390
                     /gene="proV"
                     /locus_tag="J1138_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBA8455436.1"
                     /translation="MAIKLEVKNLYKIFGEHPERAFKLLDKGLTKDRLFEKTGLSLGV
                     KDATLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINPEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1383..2447
                     /gene="proW"
                     /locus_tag="J1138_003689"
     CDS             1383..2447
                     /gene="proW"
                     /locus_tag="J1138_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBA8455437.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2504..3496
                     /gene="proX"
                     /locus_tag="J1138_003690"
     CDS             2504..3496
                     /gene="proX"
                     /locus_tag="J1138_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBA8455438.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3688..4872
                     /locus_tag="J1138_003691"
     CDS             3688..4872
                     /locus_tag="J1138_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8455439.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4996..5733
                     /locus_tag="J1138_003692"
     CDS             4996..5733
                     /locus_tag="J1138_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBA8455440.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5723..6058
                     /gene="ygaH"
                     /locus_tag="J1138_003693"
     CDS             5723..6058
                     /gene="ygaH"
                     /locus_tag="J1138_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBA8455441.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(6077..6178)
                     /locus_tag="J1138_003694"
     CDS             complement(6077..6178)
                     /locus_tag="J1138_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455442.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            6149..6679
                     /gene="emrR"
                     /locus_tag="J1138_003695"
     CDS             6149..6679
                     /gene="emrR"
                     /locus_tag="J1138_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBA8455443.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6806..7978
                     /gene="emrA"
                     /locus_tag="J1138_003696"
     CDS             6806..7978
                     /gene="emrA"
                     /locus_tag="J1138_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBA8455444.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7995..9533
                     /gene="emrB"
                     /locus_tag="J1138_003697"
     CDS             7995..9533
                     /gene="emrB"
                     /locus_tag="J1138_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBA8455445.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9597..10112)
                     /gene="luxS"
                     /locus_tag="J1138_003698"
     CDS             complement(9597..10112)
                     /gene="luxS"
                     /locus_tag="J1138_003698"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBA8455446.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10262..11818)
                     /gene="gshA"
                     /locus_tag="J1138_003699"
     CDS             complement(10262..11818)
                     /gene="gshA"
                     /locus_tag="J1138_003699"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBA8455447.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11891..12319)
                     /locus_tag="J1138_003700"
     CDS             complement(11891..12319)
                     /locus_tag="J1138_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA8455448.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLIRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12316..12882)
                     /gene="yqaB"
                     /locus_tag="J1138_003701"
     CDS             complement(12316..12882)
                     /gene="yqaB"
                     /locus_tag="J1138_003701"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBA8455449.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         3063 a         3468 c         3370 g         3237 t
ORIGIN      
        1 ccgtcaattt tgagtcacgg gccaaattac cataactaat taaaaccttg atgtaaacca
       61 ctaatatccc atttaaatga cattgagggt tgtctcagat attcactgtg ttagggtaaa
      121 aagctctttt attttgctaa ggacagccca taacgcttta attaaaccag aggaaacagc
      181 aaattgcatg gcaattaaac tcgaagtaaa gaacctgtat aagatatttg gcgagcaccc
      241 ggaacgcgca ttcaaactgc tggataaagg tctgacaaaa gatcggctgt ttgaaaaaac
      301 cggcttatcg cttggcgtaa aagacgccac tctggccatt gaagaaggcg agatatttgt
      361 catcatggga ttatccggtt cgggtaaatc cacaatggta cgccttctca atcgcctgat
      421 tgaacccacc cgcgggcaag tgctgattga tggtgtggat attgccaaaa tatccgacgc
      481 cgaactccgt gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat
      541 gccgcatatg accgtgctgg acaatactgc attcggtatg gaattggccg gaattaatcc
      601 cgaagaacgc cgggaaaaag cccttgatgc actgcgtcag gtcgggctgg aaaattatgc
      661 ccacagctac ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc
      721 gttagcgatt aatccggata tattattaat ggacgaagcc ttctcagcac tcgatccatt
      781 aattcgcacc gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat
      841 tgtctttatt tcccacgatc tcgatgaagc catgcgtatt ggcgaccgaa ttgccattat
      901 gcaaaatggt gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa
      961 tgattatgtc cgtaccttct tccgtggcgt cgatattagt caggtattca gtgcgaaaga
     1021 tattgctcgc cggacaccga acggcttaat tcgtaaaacc cccggcttcg gcccgcgttc
     1081 ggcactgaaa ttattgcagg atgaagatcg cgaatatggc tacgtcatcg aacgcggtaa
     1141 taagtttgtc ggcgctgtgt ctatcgattc gcttaaagcc gcgttaacgc agcaacaagg
     1201 tcttgatgcg gcgctgattg atgcgccgtt agcggtcgat gcacaaacgc ctcttagcga
     1261 gttgctctct catgtcggac aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca
     1321 gtatgtcggc atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa
     1381 taatggctga tcaaaataat ccgtgggata ccacgccagc ggcggacagc gccgcacaat
     1441 ccgcagacgc ctggggtaca ccggcgactg caccgactga cggcggtggt gctgactggc
     1501 tgaccagtac gcctgcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa
     1561 cgctgatccc gctcgacagt tgggtcactg aagggatcga ctgggtcgtt acccattttc
     1621 gtcccgtctt tcagggcgtg cgcgttccgg ttgattacat cctcaacggt ttccagcaat
     1681 tgctgctggg tatgcccgcg ccggtggcga ttatcgtttt cgctctcatc gcctggcaga
     1741 tttccggggt cggaatgggc gtggcgacgc tggtttcgct aattgccatc ggcgcaatcg
     1801 gtgcctggtc acaggcaatg gttaccctgg cgctggtgtt aacagccctg ctgttctgta
     1861 tcgtcatagg tttgccgttg gggatctggc tggcgagaag tccgcgagcg gcgaaaatta
     1921 ttcgtccact gcttgatgcc atgcagacca cgcccgcgtt tgtttatctg gtgccaatcg
     1981 tcatgctgtt tggtatcggc aacgtgccgg gcgtggtggt gacgatcatc tttgcgctgc
     2041 cgccgattat ccgtctgacg attctgggaa ttaaccaggt tccggcggat ctgattgaag
     2101 cctcgcgctc attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg
     2161 cgatgccaac cattatggcg ggcgttaacc agactctgat gctggccctt tctatggtgg
     2221 tcatcgcctc gatgattgcc gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc
     2281 gtctggatat ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc
     2341 tcgaccgcct gacgcaggcc gttgggcgcg actcacgcag tcgtggcaac cgtcgctggt
     2401 acaccactgg ccctgtcggt ctgctgaccc gcccattcat taagtaactc tgcacttgcc
     2461 cggtgacgcc gggcatatca ccccgccaaa aaaaggaata acaatgcgac atagcgtact
     2521 ttttgcgaca gcgtttgcca cgcttatctc tacacaaact tttgctgccg acctgccggg
     2581 caaaggcatt actgttaatc cagttcaaag caccatcacc gaagaaacct tccagacact
     2641 gctggtcagt cgtgcgctgg agaaattagg ttataccgtc aataaaccca gcgaagtgga
     2701 ttacaacgtt ggctacacct cacttgcttc cggcgatgca accttcaccg cagtgaactg
     2761 gacgccactg catgacaaca tgtacgaagc tgccggtggc gataagaaat tttatcgtga
     2821 aggagtattt gttaacggcg cggcacaggg ttacctgatc gataagaaaa ctgccgacca
     2881 gtataaaatt accaacatcg cgcaacttaa agatccgaag atcgccaaac tgttcgatac
     2941 caacggcgac ggtaaagcgg atttaaccgg ctgtaaccca ggctggggct gcgaaggtgc
     3001 gatcaaccac cagcttgccg cgtatggact gaccaatacc gtgacgcata atcaggggaa
     3061 ctacgcagcg atgatggctg acaccatcag tcgttacaaa gagggtaagc cggtgtttta
     3121 ctacacctgg acgccgtact gggtgagtaa cgaactgaaa ccggggaaag atgtggtctg
     3181 gttgcaggtg ccgttctccg cactgccggg cgataaaaat gccgatacca aactgccgaa
     3241 tggcgcgaat tacggcttcc cggtcagtac catgcatatc gttgccaaca aagcctgggc
     3301 cgagaaaaac ccggcagcag cgaaactgtt tgccattatg cagttaccag tggctgatat
     3361 taacgcccag aacgccatta tgcatgacgg caaagcctca gaaggcgata ttcagggcca
     3421 cgttgatggc tggatcaaag cccaccagca gcagttcgat ggctgggtga atgaggcgct
     3481 ggcagcgcag aagtaatttt tattcgggcg gataaggcgt ttttgcctca tccgccgctc
     3541 tgtacacaat gcctgatgcg acgctggtgc gtcttatcat acctacaaac cacaccgcac
     3601 atttcaacaa tctatctttc atcccatatt catcaacatc cgctattatt gatttccagc
     3661 ttaatcatca cctgatgaac aaaaataatg actaaaccta atcatgagct tagcccggcg
     3721 ctgatcgtgc tgatgtctat cgccaccggc ctggcggtag ccagtaacta ttacgcccag
     3781 ccattgctcg acaccatcgc gcgtaacttt tccctttccg ccagttcggc aggctttatt
     3841 gttaccgccg cgcagttggg ctatgcagca ggtctgctgt ttcttgttcc cctcggtgat
     3901 atgtttgaac gccgccgcct gattgtctcg atgaccttac tggcggcatg cggcatgtta
     3961 atcactgcca gcagtcagtc gctggcgatg atgatcctcg gtacagcatt aaccggttta
     4021 ttttcagtcg tggcacaaat tctggttcca ctggcagcga cgctggcttc accggacaaa
     4081 cgcggcaaag tggttggcac cattatgagc ggtctgctat tggggatctt actcgcacgg
     4141 accgttgccg gattgctggc gaatctcggc ggctggcgca ccgtcttttg ggttgcttcg
     4201 atgttaatgg cactgatggc gctggcatta tggcgtggtc tgccacaaat gaaatcagaa
     4261 acccacctca actacccaca gttgttgggt tccgttttca gcatgtttat cagcaataag
     4321 atcctgcgca cccgcgcgtt gctgggctgc ctgacctttg ccaacttcag cattctctgg
     4381 acctcaatgg cctttttgct tgccgctcca ccttttaact acagcgatgg tgtaattggt
     4441 ctgtttgggc ttgcgggagc tgccggagcg ttgggcgctc gtccggcggg cggttttgcc
     4501 gataagggca aatcgcacca caccacaact ttcggtctgc tgctgctgtt actttcatgg
     4561 ctggcaatct ggtttggtca cacttccgta ctggcgctga ttatcggcat cctggtgctg
     4621 gacctcaccg tgcagggcgt gcatatcact aaccagacgg taatttatcg aatacaccct
     4681 gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg cggtgccgct
     4741 ggttcgctaa tttcagcctc agcctggcaa catggcagtt gggctggcgt gtgtctggct
     4801 ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca tcgtcaggaa
     4861 gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag ggtgttaaga
     4921 agcccatcag tctgataagg ttaagatatt cattcagtct atttataata ttaacaatcg
     4981 ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc ggcgaccttc
     5041 atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt ggcctttgcg
     5101 ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt tttctcctgc
     5161 atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc cgggagtagt
     5221 ttgtgggttg ctgcactgac cgtcatggca atggatgttc gccatgtttt gtatggcccg
     5281 tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct gtgggcgttt
     5341 ggcctgacgg atgaggtttt tgccgccgca accgcaaaac tggtacgcaa taatcgccgc
     5401 tggagcgaaa actggatgat cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt
     5461 acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc cgttgaagca
     5521 gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc tttccagcgc
     5581 aaacaatctc tttgcgttac cgcagcgtta gttggtgccc ttgcaggcgt aacgctattt
     5641 tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc gttaatccag
     5701 gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt tactggttgg
     5761 cgcggcgaat tattgcttcc gctatctgcc gctgcgcctg cgtgtgggta atgcccgccc
     5821 aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct cgatatgcgc
     5881 tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt tcgtgcccac
     5941 actggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca ttatcatccc
     6001 aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga ttatataacc
     6061 cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc gttactatat
     6121 cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca ttgaacaaat
     6181 gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc ttctgacccg
     6241 tctttgcatg catatgcaaa gcaagctgct ggaaaaccgc aataaaatgc tgaaggctca
     6301 ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg aaaaccacag
     6361 tattcagcct tctgaattga gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat
     6421 tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt gaaagcgata acgatcgccg
     6481 ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg ttttaccgcc
     6541 gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa aagaccagct
     6601 cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacagg acggtgtggt
     6661 tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa
     6721 ataaccggcc agcatcgtaa catgctggcc tttttggcaa gcaggtcggc tcagccgatg
     6781 agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa ccccgcagca
     6841 accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct tgctctttat
     6901 aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt tcgaagaaac
     6961 cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt
     7021 gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgacgtgc tggtcactct
     7081 cgacccgaca gatgctcgcc aggcatttga aaaagccaaa actgcactgg cttccagcgt
     7141 tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata ttgaggtgca
     7201 aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc
     7261 caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgttacca gtgcccaggc
     7321 acaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc tggggactaa
     7381 actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg cctggctggc
     7441 gctggagcgt acccgtattg tcagtccgat gaccggttat gtctcccgcc gcgcggtaca
     7501 gcctggggcg caaattagcc caacgacgcc gctgatggcg gtcgttccag ccaccaatat
     7561 gtgggtggat gccaacttta aagagacgca gattgccaat atgcgtatcg gtcagccggt
     7621 caccatcacc accgatattt acggcgatga tgtgaaatac accggaaaag tggttggtct
     7681 ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcaa ccggtaactg
     7741 gatcaaagtc gttcagcgtc tgcctgttcg tattgaactg gaccagaaac agctcgagca
     7801 atacccgctg cgtatcggtt tgtccacgct ggtgagcgtc aataccacta accgtgacgg
     7861 tcaggtactg gcaaataaag tacgttccac accggtagcg gtaagcaccg cgcgtgaaat
     7921 cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg ctggctaatc
     7981 cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcacaactg gtcattatga
     8041 cgattgcgct gtcactggcg acattcatgc aggtgctgga ctccaccatt gctaacgtgg
     8101 cgatccccac tatcgccggg aatctgggct catcactcag tcagggaacg tgggtgatca
     8161 cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg gcaaagcgcg
     8221 tcggggaagt gaaattgttc ctctggtcaa ccatcgcctt tgctattgcg tcgtgggcgt
     8281 gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag gggattgtcg
     8341 ccgggccgct gatcccgctt tcgcaaagtc tattgctgaa taactacccg ccagccaaac
     8401 gctcgatcgc gctggcgttg tggtcgatga cggtgattgt tgcgccaatt tgcggcccga
     8461 tcctcggcgg gtatatcagc gataattacc actggggctg gatattcttc atcaacgtac
     8521 caattggcgt ggcggtggtg ttgatgaccc tgcaaaccct gcgcggacgt gaaacccgca
     8581 ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt atcggcagcc
     8641 tgcagattat gctcgaccgc ggtaaagagc tggactggtt ctcatcacag gaaattatca
     8701 tcctcaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag ctgaccgacg
     8761 ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc ggctgcttgt
     8821 gtatcagcct cgcgtatatg ctctacttcg gcgcgattgt tctgctgccg cagttgttgc
     8881 aggaggtcta cggttacacg gcgacgtggg cagggctggc ctctgcgccg gtaggaatta
     8941 ttccggtgat cctgtcgccg attatcggcc gcttcgcgca taaactggat atgcgccgac
     9001 tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc tatacctttg
     9061 aaccgggtat ggattttggc gcgtcggcct ggccacagtt tatccagggt tttgcggtgg
     9121 cctgcttctt tatgccgctg accaccatta cgctgtccgg tttgccaccg gaacgactgg
     9181 cggcggcatc gagcctctcc aactttacgc gaacgctggc ggggtctatc ggcacgtcga
     9241 taaccacgac catgtggacc aaccgcgagt cgctgcacca tgcacagttg actgagtcgg
     9301 taaacccgtt caacccgaat gctcaggcga tgtacagtca actggaaggg cttggtatga
     9361 cgcaacagca ggcatcaggc tggattgccc agcagataac caatcagggg ctgattattt
     9421 ctgccaatga gatcttctgg atgtcagccg ggatattcct cgtcttgctg gggctggtgt
     9481 ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac taagtacaac
     9541 taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt tactgactag
     9601 atgtgcagtt cctgcaactt ctctttcggc agcgccagtt cttcgttgct gttgatgcgc
     9661 acgtcacgtt ccagaatgtt acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg
     9721 taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg cactttcagc
     9781 acgtcttcca ttgccgcttt ccaggcatca gcgacacgct gctcatctgg cgtaccaatc
     9841 agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca
     9901 ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt atggatccct
     9961 ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg
    10021 tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta
    10081 tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa
    10141 attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat
    10201 catcatccct gaattcagag atgaaatttt ggccactcac gagtggcctt tttcttttcc
    10261 gtcaggcgtg cttttccagc cacaccgcaa acggttcggt atcagcggct tccatttcct
    10321 gctgacggcg ttcagacgcc tcgcgctcgg cgacaaaatc ctcttcgcgc agaatttcca
    10381 gcggctcttc acgcagcaga ttacggtacg cttcagcaaa cgctttgcct gttccgccaa
    10441 taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga
    10501 agcaggcgac cagttcatca cacactttct gatacgcttc gccgccgtta atgctatcca
    10561 gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc ggtaacggga
    10621 actgcgcagt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct tcgaggatca
    10681 cccggttcca gttaacgcgt gtacaggcaa gttcgctact gctcatttcc ggtgcgtcag
    10741 ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgttgttca tctacaccaa
    10801 tcggcgagaa cgggttgata tccagcgagc ggacttcaat atattcaatg ccgccacgta
    10861 acagcgcatc agaaggcgac tcaccactgc gggtaacgcg tttgggacga atcggcgcgt
    10921 acagttcgtt ttcaatctgc agcacattgc tgttgatttg cagccgctta ccgtctttct
    10981 caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcca
    11041 cgtactcgta gagatcattg aaggtgatac caagattgct ttgcgattta ttggtatagc
    11101 caagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg cactcggttt
    11161 tctcaaacgg cagcgacgtt ggttttcctt gcaagaaaga agaacaaatc gctggagacg
    11221 caccaaacag ataaggaatg acccaaccga agcgatagta gttacggata acacggaaat
    11281 aacccgcaga tattttctct ttggcatcag cgcccgagat atcaccgcac ttcgcttgcc
    11341 agaatgccat tggcaaagag aaattatagt gcacgccgga aatggtttgc atcagcgcgc
    11401 cgtagcgatt tttcagccct tcacggtaca gcgttttaaa gcgaccggtg ttagaagtgc
    11461 cgtactgtgc cagttcgatg tcctgacctt ctgcgatata gcatggcata cttaacggcc
    11521 acatccgctc atcgcccata ttgcgcgccg tatagcgatg cagatcgcgc ataaaggtca
    11581 gcatatgttc aatatcacca tccactggtg taataaattc cagcaatgct tccgcaaaat
    11641 cggtagtaat ccatttatgg gtcagtgcag aacctaatgc ttcaggatga cctgttgttg
    11701 ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct
    11761 ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat
    11821 tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc
    11881 aggcctacaa ttagtgccac cacatcatgc cctgaacggt agctgctgca actgcaacat
    11941 agcgtaacgc tttaccaagg cacaaaaaaa agattaccgg tccccacgag atgcgcatcc
    12001 atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga
    12061 ctgcaccata gcgtatcagc cagccggtag ctttctcttg ccagcgcgat gttttacgca
    12121 atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg
    12181 ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc accagcacga
    12241 cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa
    12301 gcgataacgc ttcactcaca gcaagcgaac atccacggcg tccatgcctg ctgcacgggc
    12361 cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat
    12421 acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga cgtgatcggc
    12481 agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgt gccagcaatg cctcagcgat
    12541 ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccagc ttttcaccac
    12601 ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg cttctgtttt
    12661 ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa tcgcctgagc
    12721 aatacgccag gtgggcgagc cattaagcgc aatcatcgcc tgaacgtcgt actgaagacc
    12781 gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctcggtat ccaggatggt
    12841 gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct cgcaagtaga
    12901 aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggag aaggagaaga
    12961 aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa ggaaattttt
    13021 tggagaagat ggtgcatccg ggaggatgac tcactggcgt tcgcccttcg ggccgttgct
    13081 ggcgtgcggt taatatcgct acctcttcga taccttcatt gctgaaggtt acggagaa
//
LOCUS       DADTFG010000138        13136 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.139, whole genome shotgun sequence.
ACCESSION   DADTFG010000138 DADTFG010000000
VERSION     DADTFG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.139"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(84..191)
                     /gene="rprA"
                     /locus_tag="J1138_003702"
     ncRNA           complement(84..191)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="J1138_003702"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(377..1489)
                     /gene="ydiK"
                     /locus_tag="J1138_003703"
     CDS             complement(377..1489)
                     /gene="ydiK"
                     /locus_tag="J1138_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBA8455450.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVGQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            1703..4759
                     /locus_tag="J1138_003704"
     CDS             1703..4759
                     /locus_tag="J1138_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBA8455451.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALSAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4756..5166
                     /gene="menI"
                     /locus_tag="J1138_003705"
     CDS             4756..5166
                     /gene="menI"
                     /locus_tag="J1138_003705"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBA8455452.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5266..5454
                     /locus_tag="J1138_003706"
     CDS             5266..5454
                     /locus_tag="J1138_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455453.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6003..6371
                     /gene="sufA"
                     /locus_tag="J1138_003707"
     CDS             6003..6371
                     /gene="sufA"
                     /locus_tag="J1138_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBA8455454.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6380..7867
                     /gene="sufB"
                     /locus_tag="J1138_003708"
     CDS             6380..7867
                     /gene="sufB"
                     /locus_tag="J1138_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBA8455455.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7877..8623
                     /gene="sufC"
                     /locus_tag="J1138_003709"
     CDS             7877..8623
                     /gene="sufC"
                     /locus_tag="J1138_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBA8455456.1"
                     /translation="MLSIKDLHVSVEDKAILRGLGLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8598..9869
                     /gene="sufD"
                     /locus_tag="J1138_003710"
     CDS             8598..9869
                     /gene="sufD"
                     /locus_tag="J1138_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBA8455457.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVCIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDCQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9866..11086
                     /gene="sufS"
                     /locus_tag="J1138_003711"
     CDS             9866..11086
                     /gene="sufS"
                     /locus_tag="J1138_003711"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBA8455458.1"
                     /translation="MTYSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLEMLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11099..11515
                     /gene="sufE"
                     /locus_tag="J1138_003712"
     CDS             11099..11515
                     /gene="sufE"
                     /locus_tag="J1138_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBA8455459.1"
                     /translation="MALLPYKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11664..12668
                     /gene="ldtE"
                     /locus_tag="J1138_003713"
     CDS             11664..12668
                     /gene="ldtE"
                     /locus_tag="J1138_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBA8455460.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12732..12968)
                     /locus_tag="J1138_003714"
     CDS             complement(12732..12968)
                     /locus_tag="J1138_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HBA8455461.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3319 a         3214 c         3543 g         3060 t
ORIGIN      
        1 tgctgacggc ttgaagagag tcacagtatc ttgtgcaaca ttattggaaa gagtgagggg
       61 cgaggtagcg aagcggaaaa atgttaaaaa aaagcccatc gtgggagatg ggcaaagact
      121 acacacagca attcgttgtt tcactcaggg gatttccatg cttataaatc aatatgttga
      181 tttataaccg tgagctaata gtaggcatac ggactttttg cgtcgatcag attcgtctca
      241 ttagtttaaa aactgtaaag aatttgcgac aggtactacc agctgaatgg cggtaagcag
      301 gaaaatatgg ggaagagcaa gcaaatatca attagcaggg gttaacaatc aggcggcaga
      361 gccgcctgat gagaaattac ttattcgatt tctcgatttc gccaagttct tcgagaattt
      421 cttccggttg gtccgtcggc ggtgggactt cttccaccca cgcggcaaaa agacgccagg
      481 aaacggctaa cagaaccgga ccaataaaca ggccgatcat cccgaaagca atcaaaccac
      541 caataacgcc agagaggatc aggatcagcg gtaaatcggc acccatgcga attaacattg
      601 ggcggatgac gttatcgagc gtgccaacca caccgctcca aactaacaat accgttcccc
      661 aggtggtatc gccagtccag tagagccaga taatcgccgg aatcagtacc ggcaacggtc
      721 caagctggac aaggcaggag aggatcatta acaccgtgag caaagttgca taaggtacgc
      781 cggatacggc gaggccgata ccgccaagca ctgcctgtac taacgccgtc accacgacac
      841 ccagcgccac cgcgcggata gcttgtgccg ccagcagcac tgcggcatct ccacgaacgc
      901 ctgccagacg ggttgcaaaa tggcgaatgc cttgccccac ttgttcaccg cgccagtaca
      961 gcagggcgct gaagagaagc atcagcgcac aatgcaccat aaagcgcccg atatgcgccg
     1021 cctgcccaac gaaccaggtg gtggtggtgc caatataagg acggactttc gccatgatcg
     1081 ccgtcccccc catatccagc aaattgtgcc agcctgcata tagcttcgcg ccaatcaccg
     1141 gaatggtatt aagccacgcc agatcgggta acgtcatgtc accactggaa atggctttaa
     1201 ttagcgggcc gctgccgtcg acgatactgt taaccagcaa agcaataggg atgataaaca
     1261 ccatcactaa taacagcgtc atcaccagaa cggcgagaga gcggcgacca aacatgatct
     1321 tttgcaaacg taacaatacc ggccaggtgg cgataaccac tgtaccggcc catgcaaagc
     1381 cgagaataaa gggttgtaca atccacagac atgccacaat catgatggct aaaaacagca
     1441 ccgaaagcag aatttgtgcg acatccctgg gctgacgaac atttaccata actacttttc
     1501 accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac gcgaaactca cctgcataat
     1561 gatgagcaat ttcagcggtt tttaacaggt cgattctgcc cttaattctg atgggcgtgt
     1621 aagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa cgattaatct ctcatcacag
     1681 accgcaggaa agggtcaata taatgattcc acagatttcc caggcacccg gcgtcgttca
     1741 actggtgctt aattttttgc aagagctgga gcaacagggt tttaccggcg atacggcgac
     1801 aagttatgcc gatcgtctga caatgtcgac tgacaacagt atttaccaac ttctccccga
     1861 tgcggtggta tttccgcgtt caaccgcaga tgtagcgctg atcgcccgtc ttgccgcgca
     1921 ggaacgctat tcatcgttga tctttacccc ccgcggcggc ggcaccggca ctaacggaca
     1981 ggcgctcaac caggggatta ttgttgatat gtcccgccat atgaaccgca tcatcgaaat
     2041 taaccctgaa gagggctggg tgcgcgttga ggccggggtg attaaagacc aactcaatca
     2101 gtacctgaaa ccctttggct acttttttgc gccagaactt tcgaccagta accgggcaac
     2161 gctcggcggg atgatcaata ccgatgcatc cggtcaggga tcgctggtct atggcaaaac
     2221 gtcagatcac gtgcttggcg tacgcgcggt gttgttgggg ggcgatattc tcgatacgca
     2281 acctttaccc gtcgaactgg cggaaacgct gggtaaatcc aataccacaa tcgggcgaat
     2341 ttataacacg gtttatcaac gttgccgtca gcaacgccag ttaatcatcg acaacttccc
     2401 caagcttaac cgctttctta ccggttacga tctgcgtcat gtctttaacg atgagatgac
     2461 cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg acgctggcct ttattaccga
     2521 agcgcgactg gatattacgc gcttgcctaa agtacgccgt ctggtgaacg tcaaatatga
     2581 ctcttttgac tccgcgctgc gtaacgcgcc gtttatggtt gaggcgcggg cgctttcggt
     2641 agagacggtg gactcaaaag tgctgaatct ggcgcgggaa gatattgtct ggcattccgt
     2701 cagcgagttg attaccgatg tgcctgataa agagatgctc gggctgaaca ttgtggaatt
     2761 tgccggcgat gatgaggcgc tgattgacga gcgagttaat gcactctgtg tgcggcttga
     2821 tgagctgatt gccagccaac aggcaggagt gatcggctgg caggtgtgcc gcgagctggc
     2881 gggtgttgaa cgtatctatg cgatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa
     2941 aggtgctgct aagccaattc cgtttgctga ggatacctgc gtaccgccgg aacacctggc
     3001 ggattatatt gctgaatttc gcgcgctgct cgacagccac ggcttaagct acggtatgtt
     3061 cggtcacgtc gacgcaggtg tcttgcacgt ccgtccggca ctggatatgt gcgatccgca
     3121 acaagagatt ttgatgaagc aaatctctga tgatgtagtg gcgcttaccg cgaaatacgg
     3181 tggtttgttg tggggcgaac acggcaaagg ttttcgcgcc gaatacagcc cggcgttttt
     3241 cggtgaggaa ctttttgcag aactgcgtaa agtgaaagcg gcatttgacc cgcataaccg
     3301 actcaacccc gggaagattt gcccgccaga aggtctcgat gcgccgatga tgaaagtgga
     3361 cgcggtgaag cgcggtacat tcgatcgaca gatccccatt gcggtacgcc agcagtggcg
     3421 tggtgcaatg gagtgtaacg gcaacggttt atgcttcaac tttgatgctc gtagtccgat
     3481 gtgcccgtcg atgaagatca cccagaaccg gattcattca ccgaaagggc gcgcaacgct
     3541 ggtacgtgaa tggctgcgtt tgttggcgga tcgcggcgtt gacccactca aactggaaca
     3601 agaactgcct gaatccggcg tcagtttgcg gacgttaatt gcccgcacgc gcaatagttg
     3661 gcatgcgaat aaaggcgaat atgacttctc acacgaagtc aaagaggcga tgtcgggctg
     3721 tctggcctgt aaagcttgtt cgactcagtg ccccatcaaa attgatgtgc cggagtttcg
     3781 ctctcgtttc ctgcaactct atcacacccg ttatttacgc ccgctgcgcg accacctcgt
     3841 cgctacggtt gagagttacg caccgctgat ggcacgcgcg ccgaaaacct ttaacttctt
     3901 cattaaccag ccgctggtgc gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc
     3961 gctgctgtca gtcccctcgc tacaacaaca aatggtgggg catcgctcgg caaacatgac
     4021 gctggaacag cttgaagctc tcagtgcaga gcagaaagcg cgcacggtgc tggtggtgca
     4081 ggaccccttt accagctatt acgatgctca agtggtggcg gattttgtcc gtctggtcga
     4141 aaaattaggt ttccagcccg tgttactgcc attttcgcca aatggcaaag cccagcatat
     4201 caaaggtttt cttaatcgtt ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg
     4261 tatggcgaag ctgggtatgc caatggtagg cgtcgacccg gcgctggtac tttgttatcg
     4321 cgatgaatat aaactggctc tgggcgagga gcgtggcgag tttaacgtct tactggcgaa
     4381 tgaatggctg gcaagcgcac ttgagtcaca gccagtggct acagtcagcg gtgaatcatg
     4441 gtatttcttt ggtcactgta ccgaagttac cgccttgccg ggcgcgccag cacaatgggc
     4501 ggcaatattt gcccgttttg gcgcgaaact ggaaaatgtc agcgtggggt gctgcggcat
     4561 ggcagggact tacggacatg aagcgaaaaa ccataaaaat tcgctcggga tctatgaatt
     4621 atcctggcat caggctatgc agcgactgcc gcgtaaccgc tgtctggcga ccggatattc
     4681 ctgccgtagt caggtaaaac gggttgaagg cacaggggta cgccatcctg tgcaggcttt
     4741 actggagatt attaaatgat atggaaacga aaaatcaccc tggaagcact gaatgctatg
     4801 ggggaaggaa acatggtggg attgctggat attcgctttg aacatattgg tgatgacacc
     4861 cttgaagcga caatgccagt agactcacga acaaagcagc ctttcgggtt gctgcatgga
     4921 ggtgcatccg tggtactggc cgaaagtatc ggttccgttg ccggttattt atgtaccgaa
     4981 ggtgagcaaa aagtggttgg tctggaaatc aatgctaacc acgtccgctc ggcacgagaa
     5041 gggcgggtgc gcggcgtatg caaaccgttg catctcggtt cgcgtcacca ggtctggcag
     5101 attgaaatct tcgatgagaa agggcgtttg tgctgttcgt cacgattgac gaccgctatt
     5161 ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt ttggttatta tctgcacagt
     5221 aaacggtggt atactgtgca ggtcttgtct aaaagcgagg atgctatgtc tactcaactt
     5281 gatcccaccc aactggccat tgaattttta cgccgtgacc aaagcaatct ttcacctgcc
     5341 caatatctca agcgtctgaa acaattagaa cttgaatttg ctgacctgct tactctctct
     5401 tctgctgaat taaaagaaga aatctacttc gcctggcgtc tgggcgtgca ttgatcctga
     5461 tttaatgtcg cgagttcgcc aaatcggcct ggattaatct tcgtccaggt tcgagggcct
     5521 tcttaatact gcctatctac ccgcctgata acgttcaaaa ataatacaaa tcgcagtttg
     5581 tgttaaaaca gtgggttagc tttatgagat tatccttatc gccccttcaa gagctaagcc
     5641 actgtgagtg ccggagataa gcgccggatg gggtagaaac ccttaagcct gtgtcgcaca
     5701 gacttaaggg ttttcttatt tctgaacgct gcactttttt acctatccct taaatagtca
     5761 ttttcaatac aactttttct ttttcatcac agccttaatg ctccttagat aataatcatt
     5821 atctaacaat gagataccta attcttagcg tgcctgttaa cccacaaatc agggtctatg
     5881 cttattaaaa gcaggtaaaa agcatgtttt acgctaaagc ccctgcgttt gctgggttga
     5941 actgataatc attatcacta acatgctgtt atacgctgaa agcgatgaag tgaggtaaat
     6001 cgatggacat gcattcagga acctttaatc cacaggattt cgcctggcaa ggcttaacgc
     6061 tgacacccgc agcggcgata cacatccgcg aactggtggc aaagcagcct ggtatgatcg
     6121 gcgtgcgctt aggcgttaag caaacaggct gcgcgggctt tggctatgtg ctcgacagtg
     6181 ttagcgagcc ggacaaagac gatctgctgt ttgaacacga cggcgcgaag ctgtttgtcc
     6241 cgctgcaagc gatgcctttt attgatggca cggaagtcga tttcgttcgc gaaggactta
     6301 atcagatatt caaatttcac aaccctaaag cccagaatga atgtggctgt ggcgaaagct
     6361 ttggggtata ggcggtacta tgtctcgtaa tactgaagca actgacgatg tcaaaacctg
     6421 gaccggcggc ccgctgaatt ataaagaagg attcttcacc cagttagcca ccgatgagct
     6481 ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg gcgaagcgta atgagccgga
     6541 gtggatgctg gagtttcgtc tcaacgccta tcatgcatgg ctggagatgg aagaaccgca
     6601 ctggttgaaa gcgcactacg acaagctgaa ttatcaggat tacagctact actcggcacc
     6661 atcgtgcggt aattgtgacg acaactgcgc gtctgaaccc ggcgcggtgc agcaaactgg
     6721 cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt gagcagttgg gcgttcccgt
     6781 gcgggaaggc aaagaggtgg cagtggatgc cattttcgac tctgtttcgg ttgccactac
     6841 ttatcgcgaa aaactggcgg agcagggaat tattttttgt tcctttggcg aggcgatcca
     6901 cgatcacccg gaactggtgc gtaaatatct cggcaccgtg gtgccgggga atgacaactt
     6961 ctttgccgca cttaatgcgg cggtagcctc tgacggtacg tttatttatg tgcctaaagg
     7021 cgtgcgttgc ccgatggaac tttccaccta ttttcgcatt aacgcggaaa aaaccgggca
     7081 gtttgagcgc accattctgg tggccgacga agacagctat gttagctaca ttgaaggttg
     7141 ttccgctccg gtgcgtgaca gctatcagtt acacgcggcg gtggtggaag tcatcatcca
     7201 taaaaacgcc gaggtgaaat attccacggt acaaaactgg ttccctggcg ataacaacac
     7261 cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc gaaggcgaaa acagcaaaat
     7321 gtcatggacg caatcagaaa ccgggtcagc gattacgtgg aaatatccca gctgcatttt
     7381 gcgcggcgat aactccattg gtgagtttta ctcagtggcg ctgaccagcg gtcatcagca
     7441 agcggatacc ggcaccaaga tgatccacat cggtaaaaac accaaatcga ccattatctc
     7501 gaaagggatc tctgccggac atagtcagaa cagttatcgc ggcttagtga aaatcatgcc
     7561 gacggcaacc aatgcgcgca atttcactca gtgcgattca atgctgattg gcgctaattg
     7621 tggggcgcat accttcccgt atgtcgaatg tcgcaataac agcgctcagc tggagcacga
     7681 ggcaacgaca tcacgtattg gtgaagatca actgttttac tgcctgcaac gcgggatcag
     7741 cgaagaagat gccatctcga tgattgttaa cggtttttgc aaagacgtgt tctcggaact
     7801 gccgctggaa tttgccgttg aagcacaaaa actcctcgcc atcagtcttg aacacagcgt
     7861 cggataagga ataaacatgt taagtattaa agatttacac gtcagcgtgg aagataaagc
     7921 tatcctgcgt ggattaggcc tcgacgttcg tcccggcgaa gttcacgcca ttatggggcc
     7981 caatggttcg ggcaaaagta ccttatcggc aacgcttgcc gggcgagaag attatgaagt
     8041 gacgagcggc acggttgagt ttaaaggcaa agatttgctt gcgctgtcgc cggaagatcg
     8101 tgcaggtgaa ggcatcttta tggccttcca gtatccggtg gaaattcccg gcgtcagtaa
     8161 ccagtttttc ctgcaaacgg cacttaatgc ggtgcgcagc tatcgcgggc aggaaacgct
     8221 cgaccgcttt gattttcagg atttgatgga agagaaaatc gctctcctga agatgccgga
     8281 agatttatta acccgttcgg taaacgttgg tttttccggc ggcgagaaaa agcgcaacga
     8341 tattttgcaa atggcggtgc tggaaccgga gttatgcatt cttgatgagt cagactccgg
     8401 gctggatatt gacgcattaa aagtggttgc cgatggcgtg aactcgctgc gtgacggcaa
     8461 gcgttcattc atcattgtta cgcactacca acgcattctc gactacatca agcctgatta
     8521 cgtccatgtg ctgtatcagg gacgaattgt gaaatccggc gatttcacgt tggtcaaaca
     8581 actggaggag cagggttatg gctggcttac cgaacagcag taacgcactg caacagtggc
     8641 atcatttgtt tgaagctgaa ggggcgaaac gctccccgca ggcacagcag catttacaac
     8701 aattgctgcg taccggactg ccgacacgga aacatgaaaa ctggaaatat acgccgctgg
     8761 aagggctgac caatagccag tttgtctgca ttgcgggaga gatatcccca cagcagcgag
     8821 atgccttagc gttaacgtta gacgccgtgc ggctggtgtt tgtcgatgga cgttacgtgc
     8881 ccgcgctgag cgatgcaact gaaggcagcg gatatgaagt gagcattaac gacgactgtc
     8941 aggggttacc cgacgctatt caggcggaag tgtttctgca tttgacggaa agcctggcac
     9001 aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacgacc ggcaaagcca ttgctgttaa
     9061 tgcatatcac ccagggcgtg gcaggtgaag aggtgaatac tgcccattac cgacatcatc
     9121 tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca ttttgtcagc cttaatgatg
     9181 ctcgtcactt caccggcgca cggttcacta tcaacgtcgc agcgaacgcc cacttgcagc
     9241 atatcaagct ggcgtttgaa aacccggtca gtcaccactt tgcccataac gatttgttgc
     9301 tggctgacga tgccaccgca tttagccaca gtttcctgct gggtggcgca gtgttacgac
     9361 acaacaccag tacgcaactc aatggcgaaa acagcacgct gcggatcaat agcctggcga
     9421 tgccggtgaa aaacgaggtg tgtgataccc gtacctggct ggaacataat aaaggttttt
     9481 gtaacagccg acagttgcac aaaactatcg tcagcgacaa aggccgtgcg gtgtttaacg
     9541 gtctgatcaa cgtcgcgcag cacgccatca aaacggatgg tcagatgacc aacaataatc
     9601 tgctgatggg caaactggcg gaggtggata cgaaaccgca gctggaaatc tatgcagatg
     9661 atgtgaaatg cagccacggc gcgacggtgg ggcgtattga tgatgaacag atgttctatc
     9721 tgcgctcgcg cgggatcaat cagcaggatg cccagcagat gatcatttac gccttcgctg
     9781 ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca gcaggtgctg gcccgaatcg
     9841 gtcaacggct gccaggaggt gcaagatgac ttattccgtc gacaaagtgc gggccgactt
     9901 tccggtgctt tcgcgtgagg taaacggttt gccgctggct tatctcgaca gcgccgccag
     9961 tgcacagaaa ccgagccagg tgattgacgc cgaggccgag ttttatcgtc atggctacgc
    10021 ggcggtgcat cgcggtattc ataccttaag cgcccaggcg accgagaaaa tggagaacgt
    10081 gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg gaagagctgg tgttcgtccg
    10141 cggcacgacg gaagggatca atctggtcgc caatagctgg ggtaacagca atgtgcgggc
    10201 gggcgataac atcatcatca gtcagatgga gcaccacgct aacattgtcc cctggcagat
    10261 gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg ctcaaccccg acggtacgct
    10321 gcaactggag atgctaccta atctgttcga tgagaaaact cgcctgctgg caattactca
    10381 tgtctccaac gtgctgggaa cggaaaatcc gctggcggaa atgatcacgc ttgcgcacca
    10441 gcatggcgca aaagtgctgg tggatggcgc tcaggcggtg atgcatcatc cggtggatgt
    10501 tcaggcgctg gattgcgatt tttacgtgtt ttccgggcat aaactgtatg gccccaccgg
    10561 gattggcatt ctttatgtca aagaagcctt gttgcaggag atgccgccgt gggaaggggg
    10621 cggttctatg atcgccaccg tcagcctgag tgaaggcact acctggacca aagcaccttg
    10681 gcggtttgaa gccggtacac ccaataccgg gggcatcatt ggtcttggcg cggcgctgga
    10741 gtatgtgtcg gcgctggggc ttaataacat agccgagtat gaacagaatc tgatgcacta
    10801 cgcgctatca cagctggaat ctgtaccgga tctcactctc tatggcccgc agaacaggct
    10861 tggcgttatt gcttttaatc tcggtaaaca ccacgcctat gatgttggca gttttctcga
    10921 taactacggc attgctgtgc gtaccggaca tcactgcgca atgccattga tggcctatta
    10981 caacgttcct gctatgtgtc gggcgtcgct ggccatgtat aacacccatg aagaagtgga
    11041 tcgtctggtg accggcctgc aacgtattca ccgtctgctg ggataacagg gaggcactat
    11101 ggctttattg ccgtataaag aaaaattgct gcgtaatttt ttacgctgcg ccaactggga
    11161 agagaaatat ctctacatta ttgagctggg ccagcgtctg ccagaattac gcgacgaaga
    11221 cagaagccca caaaatagca ttcagggctg ccagagtcag gtgtggattg tcatgcgcca
    11281 gaatgcgcag ggaattattg aattgcaggg cgacagcgat gcggcgattg tgaaagggct
    11341 aattgcggtc gtctttattc tctacgatca gatgacgccg caggatattg tcaatttcga
    11401 tgtgcgtccg tggtttgaaa aaatggcgct cacccaacat ctcaccccct ctcgttcaca
    11461 aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc gctgcactta gctaaactaa
    11521 agggacagct ttcatccggt tggctcacat tgccggatgg tcaatgttta cactgacctg
    11581 cacaggagtg tttcggtttc aggtgaacat aagcgccatc cgacgtttca gcgtgagtct
    11641 ccggcaaata caggaggttt acaatgaaac gcgcgtcttt gcttacactc acgcttatcg
    11701 gcgcttttag cgccatccag gctgcctggg cggttgatta tccgctacca ccaaccggaa
    11761 gccgactggt tgggcaaaat caaacgtata cggtgcaaga aggggataaa aaccttcagg
    11821 ccatcgcccg acgttttgat actgcggcaa tgttgatcct tgaagccaat aacactatcg
    11881 ccccggtgcc aaaacccggt acgacgataa ctattccttc gcaactgtta ttacctgatg
    11941 cgccgcgtca ggggattatc gttaacctgg cagagctgcg cctttattat tatccgccgg
    12001 gagaaaatat cgtgcaggtt tatccgatag gtattggttt gcaggggctg gaaacgccag
    12061 tgatggaaac gcgtgtgggg cagaaaatcc ctaacccaac ctggacgcct acggcgggca
    12121 ttcgtcagcg ttcgttggag cgtggcatta aattaccgcc agtcgttcct gccgggccaa
    12181 ataacccact aggacgttac gcactgcgcc tcgcgcatgg taatggcgaa tacctcattc
    12241 atggcaccag tgcgccggac agcgtcggtt tgcgcgtcag ttcgggctgt attcgcatga
    12301 atgcgccgga tattaaagcc ttattctcca gcgtgcggac gggaacgccg gtgaaagtga
    12361 tcaacgaacc ggtgaaatac tccgtggaac cgaacgggat gcgttatgtt gaagtacatc
    12421 gaccactatc agcagaagaa cagcagaacg ttcagacaat gccatacaca ctgccagcag
    12481 gctttacgca atttaaagac aataagggtg tagatcagaa attagtcgat aaagcgttgt
    12541 atcgtcgggc agggtatccg gttgcggtga gcagtggagc aactcccgca gccagcaatg
    12601 cgccttcagt agagtcagcg cagaatggtg aaccagagca agggaatatg ttacgcgcga
    12661 cgcagtagcg gtgaatagca ggcaaaaaaa atggcgcaca gtgtgcgcca tttttcactt
    12721 cacaggtact attacttgcg gtatttagta gccatgttgt ccagacgctg gttagcacga
    12781 gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg cgttcacgtc gttgctcagc
    12841 tggtcaactt tagcgttcag agtctgaacg tcagaagaca gctgatcgat tttagcgttg
    12901 ctggagcaac ctgccagcag agtagaaccc aggattaccg cgcccagtac cagtttagta
    12961 gctttcatta ttaataccct ctagattgag ttaatctcca tgtagcgtta caagtattac
    13021 acaaagtttt ttatgttgag aatatttttt tgatgggaag gcacttattt ttgatcgttc
    13081 gctcaaagaa gcatcgaacg ctgggtttta gataaaaaaa ttgagagaaa acagga
//
LOCUS       DADTFG010000139        12523 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.140, whole genome shotgun sequence.
ACCESSION   DADTFG010000139 DADTFG010000000
VERSION     DADTFG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.140"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            25..537
                     /gene="hlyC"
                     /locus_tag="J1138_003715"
     CDS             25..537
                     /gene="hlyC"
                     /locus_tag="J1138_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin-activating
                     lysine-acyltransferase HlyC"
                     /protein_id="HBA8455462.1"
                     /translation="MNRNNPLEVLGHVSWLWASSPLHRNWPVSLFAINVLPAIRANQY
                     ALLTRDNYPVAYCSWANLSLENEIKYLNDVTSLLAEDWTSGDRKWFIDWIAPFGDNGA
                     LYKYMRKKFPDELFRAIRVDPKTHVGKVSEFHGGKIDKQLANKIFKQYHHELITEVKN
                     KSDFNFSLTG"
     gene            549..3623
                     /gene="hlyA"
                     /locus_tag="J1138_003716"
     CDS             549..3623
                     /gene="hlyA"
                     /locus_tag="J1138_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin hemolysin HlyA"
                     /protein_id="HBA8455463.1"
                     /translation="MPTITTAQIKSTLQSAKQSAANKLHSAGQSTKDALKKAAEQTRN
                     AGNRLILLIPKDYKGQGSSLNDLVRTADELGIEVQYDEKNGTAITKQVFGTAEKLIGL
                     TERGVTIFAPQLDKLLQKYQKAGNKLGGSAENIGDNLGKAGSVLSTFQNFLGTALSSM
                     KIDELIKKQKSGGNVSSSELAKASIELINQLVDTAASLNNNVNSFSQQLNKLGSVLSN
                     TKHLTGVGNKLQNLPNLDNIGAGLDTVSGILSVISASFILSNADADTGTKAAAGVELT
                     TKVLGNVGKGISQYIIAQRAAQGLSTSAAAAGLIASVVTLAISPLSFLSIADKFKRAN
                     KIEEYSQRFKKLGYDGDSLLAAFHKETGAIDASLTTISTVLASVSSGISAAATTSLVG
                     APVSALVGAVTGIISGILEASKQAMFEHVASKMADVIAEWEKKHGKNYFENGYDARHA
                     AFLEDNFKILSQYNKEYSVERSVLITQQHWDTLIGELAGVTRNGDKTLSGKSYIDYYE
                     EGKRLEKKPDEFQKQVFDPLKGNIDLSDSKSSTLLKFVTPLLTPGEEIRERRQSGKYE
                     YITELLVKGVDKWTVKGVQDKGSVYDYSNLIQHASVGNNQYREIRIESHLGDGDDKVF
                     LAAGSANIYAGKGHDVVYYDKTDTGYLTIDGTKATEAGNYTVTRVLGGDVKVLQEVVK
                     EQEVSVGKRTEKTQYRSYEFTHINGTELTETDNLYSVEELIGTNRADKFFGSKFTDIF
                     HGADGDDHIEGNDGNDRLYGDKGNDTLRGGNGDDQLYGGDGNDKLTGGVGNNYLNGGD
                     GDDELQVQGNSFAKNVLSGGKGNDKLYGSEGADLLDGGEGNDLLKGGYGNDIYRYLSG
                     YGHHIIDDDGGKEDKLSLADIDFRDVAFRREGNDLIMYKAEGNVLSIGHKNGITFRNW
                     FEKESGDISNHQIEQIVDKGGRVITPDSLKKALEYQQSNNKASYVYGNDALAYGSQDN
                     LNPLINEISKIISAAGNFDVKEERSAASLLQLSGNASDFSYGRNSITLTASA"
     gene            3695..5818
                     /locus_tag="J1138_003717"
     CDS             3695..5818
                     /locus_tag="J1138_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HBA8455464.1"
                     /translation="MDSCHKIDYGLYALEILAQYHNVSVNPEEIKHRFDTDGTGLGLT
                     SWLLAAKSLELKVKQVKKTIDRLNFISLPALVWREDGRHFILTKVSKEANRYLIFDLE
                     QRNPRVLEQSEFEALYQGHIILIASRSSVAGKLAKFDFTWFIPAIIKYRRIFIETLVV
                     SVFLQLFALITPLFFQVVMDKVLVHRGFSTLNVITVALSVVVVFEIILSGLRTYIFAH
                     STSRIDVELGAKLFRHLLALPISYFESRRVGDTVARVRELDQIRNFLTGQALTSVLDL
                     LFSFIFFAVMWYYSPKLTLVILFSLPCYAAWSVFISPILRRRLDDKFSRNADNQSFLV
                     ESVTAINTIKAMAVSPQMTNIWDKQLAGYVAAGFKVTVLATIGQQGIQLIQKTVMIIN
                     LWLGAHLVISGDLSIGQLIAFNMLAGQIVAPVIRLAQIWQDFQQVGISVTRLGDVLNS
                     PTESYHGKLALPEINGDITFRNIRFRYKPDSPVILDNINLSIKQGEVIGIVGRSGSGK
                     STLTKLIQRFYIPENGQVLIDGHDLALADPNWLRRQVGVVLQDNVLLNRSIIDNISLA
                     NPGMSVEKVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNNP
                     KILIFDEATSALDYESEHIIMRNMHKICKGRTVIIIAHRLSTVKNADRIIVMEKGKIV
                     EQGKHKELLSEPESLYSYLYQLQSD"
     gene            5837..7273
                     /gene="hlyD"
                     /locus_tag="J1138_003718"
     CDS             5837..7273
                     /gene="hlyD"
                     /locus_tag="J1138_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin T1SS ABC transporter subunit
                     HlyD"
                     /protein_id="HBA8455465.1"
                     /translation="MKTWLMGFSEFLLRYKLVWSETWKIRKQLDTPVREKDENEFLPA
                     HLELIETPVSRRPRLVAYFIMGFLVIAVILSVLGQVEIVATANGKLTLSGRSKEIKPI
                     ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQTRLEQTRYQILSRSI
                     ELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLT
                     ILARINRYENLSRVEKSRLDDFRSLLHKQAIAKHAVLEQENKYVEAANELRVYKSQLE
                     QIESEILSAKEEYQLVTQLFKNEILDKLRQTTDSIELLTLELEKNEERQQASVIRAPV
                     SGKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEA
                     FPYTRYGYLVGKVKNINLDAIEDQKLGLVFNVIVSVEENDLSTGNKHIPLSSGMAVTA
                     EIKTGMRSVISYLLSPLEESVTESLHER"
     gene            complement(7645..7953)
                     /locus_tag="J1138_003719"
                     /pseudo
     CDS             complement(7645..7953)
                     /locus_tag="J1138_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7970..9046)
                     /locus_tag="J1138_003720"
     CDS             complement(7970..9046)
                     /locus_tag="J1138_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc21 family transposase"
                     /protein_id="HBA8455466.1"
                     /translation="MAGKVTETAVVGGVDTHKDLHVAAVVDQNNKVLGTQFFSTTRQG
                     YRQMLAWMTSFGALKRIGVECTGTYGSGLLRYLQNAGLDVLEVTAPDRMERRKRGKSD
                     TIDAECAAHAAFSGIRTVTPKTRNGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDDKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRQINSIPITA"
     gene            complement(9328..10184)
                     /locus_tag="J1138_003721"
                     /pseudo
     CDS             complement(9328..10184)
                     /locus_tag="J1138_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10476..10649)
                     /locus_tag="J1138_003722"
                     /pseudo
     CDS             complement(10476..10649)
                     /locus_tag="J1138_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(10870..11079)
                     /locus_tag="J1138_003723"
     CDS             complement(10870..11079)
                     /locus_tag="J1138_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455467.1"
                     /translation="MADSADIAYENEQFSMSIRLKNRIRNRLPETGFCYNCGEPVKTG
                     LFCDGDCREDYEKRERFGKINTDNV"
     gene            complement(11218..11586)
                     /locus_tag="J1138_003724"
     CDS             complement(11218..11586)
                     /locus_tag="J1138_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455468.1"
                     /translation="MAESGFISLELSRSQAPVTISKTAILFAEADNDTPESTHLHCVG
                     GITHTVNGVFNDIAELLPRFIKTHRRDNGQPAVAIHDWNVSYIEKGNDASAVVYFTES
                     GSSITVRESYEDIIAKFHAL"
     gene            complement(12090..12323)
                     /locus_tag="J1138_003725"
     CDS             complement(12090..12323)
                     /locus_tag="J1138_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HBA8455469.1"
                     /translation="MSEYMKNEILEFLNRHNGRKTAEIAEALAVTDYQARYYLLLLEK
                     EGMVQRSPLRRGMATYWFLKGEKQAGQSCSSTT"
BASE COUNT         3556 a         2503 c         2903 g         3561 t
ORIGIN      
        1 tcaatggaga tagtatcatt tataatgaac agaaacaatc cattagaggt tcttgggcat
       61 gtatcctggc tctgggccag ttccccacta cacagaaact ggccagtctc tttgtttgca
      121 ataaatgtat tacctgcaat acgggctaac caatatgctt tattaacccg ggataattac
      181 cctgttgcat attgtagttg ggctaattta agtttagaaa atgaaattaa atatcttaat
      241 gatgttactt cattactcgc agaagactgg acttcaggtg atcgtaaatg gttcattgac
      301 tggattgctc ctttcgggga taacggtgcc ctgtacaaat atatgcgaaa aaaattccct
      361 gatgaactat tcagagccat cagggtggat cccaaaactc atgttggtaa agtatcagaa
      421 tttcacggag gtaaaattga taaacagtta gcgaataaaa tttttaaaca atatcaccac
      481 gagttaataa ctgaagtaaa aaacaagtca gatttcaatt tttcattaac aggttaagag
      541 gtaattaaat gccaacaata accactgcac aaattaaaag cacactgcag tctgcaaagc
      601 aatccgctgc aaataaattg cactcagcag gacaaagcac gaaagatgca ttaaaaaaag
      661 cagcagagca aacccgcaat gcgggaaaca gactcatttt acttatccct aaagattata
      721 aaggacaggg ttcaagcctt aatgaccttg tcaggacggc agatgaactg ggaattgaag
      781 tccagtatga tgaaaagaat ggcacggcga ttactaaaca ggtattcggc acagcagaga
      841 aactcattgg cctcaccgaa cggggagtga ctatctttgc accacaatta gacaaattac
      901 tgcaaaagta tcaaaaagcg ggtaataaat taggcggcag tgctgaaaat ataggtgata
      961 acttaggaaa ggcaggcagt gtactgtcaa catttcaaaa ttttctgggt actgcacttt
     1021 cctcaatgaa aatagacgaa ctgataaaga aacaaaaatc tggtggcaat gtcagttctt
     1081 ctgaactggc aaaagcgagt attgagctaa tcaaccaact cgtggacaca gctgccagcc
     1141 ttaataataa tgttaactca ttttctcaac aactcaataa gctgggaagt gtattatcca
     1201 atacaaagca cctgaccggt gttggtaata agttacagaa tttacctaac cttgataata
     1261 tcggtgcagg gttagatact gtatcgggta ttttatctgt gatttcagca agtttcattc
     1321 tgagcaatgc agatgcagat accggaacta aagctgcagc aggtgttgaa ttaacaacga
     1381 aagtactggg taatgttgga aaaggtattt ctcaatatat tatcgcacag cgtgcagcac
     1441 aggggttatc tacatctgct gctgctgccg gtttaattgc ttctgttgtg acactggcaa
     1501 ttagtccact ctcattcctg tccattgccg ataagtttaa acgtgccaat aaaatagagg
     1561 agtattcaca acgattcaaa aaacttggat acgatggtga cagtttactt gctgctttcc
     1621 acaaagaaac aggagctatt gatgcatcgt taacaacgat aagcactgtt ctggcttcag
     1681 tatcttcagg tattagtgct gctgcaacga catctctggt tggtgcaccg gtaagcgcgc
     1741 tggtaggggc tgttacgggg ataatttcag gtatccttga ggcttcaaaa caggcaatgt
     1801 ttgaacatgt cgccagtaaa atggccgatg ttattgctga atgggagaaa aaacacggca
     1861 aaaattactt tgaaaatgga tatgatgccc gccatgctgc atttttagaa gataacttta
     1921 aaatattatc tcagtataat aaagagtatt ctgttgaaag atcagtcctc attactcagc
     1981 aacattggga tacgctgata ggtgagttag ctggtgtcac cagaaatgga gacaaaacac
     2041 tcagtggtaa aagttatatt gactattatg aagaaggaaa acgtctggag aaaaaaccgg
     2101 atgaattcca gaagcaagtc tttgacccat tgaaaggaaa tattgacctt tctgacagca
     2161 aatcttctac gttattgaaa tttgttacac cattgttaac tcccggtgag gaaattcgtg
     2221 aaaggaggca gtccggaaaa tatgaatata ttaccgagtt attagtcaag ggtgttgata
     2281 aatggacggt gaagggggtt caggataagg ggtctgtata tgattactct aacctgattc
     2341 agcatgcatc agtcggtaat aaccagtatc gggaaattcg tattgagtca cacctgggag
     2401 acggggatga taaggtcttt ttagctgccg gctcagccaa tatctacgca ggaaaggggc
     2461 atgatgttgt ttactatgat aaaacagata ccggttatct gaccattgat ggtacaaaag
     2521 caactgaagc cggtaattac acggtaacac gtgtacttgg tggcgatgtt aaggttttac
     2581 aggaagttgt gaaggagcag gaggtttcag ttggtaaaag gactgaaaaa acacaatatc
     2641 gaagctatga attcactcat attaatggca cagaattaac cgagacagat aacttatact
     2701 ccgtggagga gctcatcgga accaaccgtg ctgataagtt ttttggcagc aaatttacag
     2761 atatcttcca tggcgcggat ggtgatgacc atatagaagg aaatgatggg aatgaccgct
     2821 tatatggtga taaaggtaat gacacgctga ggggcggaaa cggggatgac cagctctatg
     2881 gcggtgatgg taacgataag ctaaccggag gtgtgggtaa taactacctt aatggcggag
     2941 acggggatga tgagcttcag gttcagggaa attcttttgc taaaaatgta ttatccggtg
     3001 gaaaaggtaa tgacaagctg tacggcagtg agggggcaga tctgcttgat ggtggagagg
     3061 ggaatgatct cctgaaaggc ggatatggta atgatattta tcgttatctg tcaggatatg
     3121 gccatcatat tattgacgat gatgggggga aagaggataa actcagtttg gctgatattg
     3181 atttccggga tgtggccttc aggcgagaag gtaatgacct catcatgtat aaagctgaag
     3241 gtaatgttct ttccattggt cataaaaatg gtattacatt caggaactgg tttgaaaaag
     3301 agtcaggtga tatctctaat caccagatag agcagattgt tgataaaggt ggccgggtaa
     3361 tcacacctga ttcccttaaa aaggcacttg agtatcaaca gagtaataat aaggcaagtt
     3421 atgtgtatgg gaatgatgca ttagcctatg gaagtcagga taatcttaat ccattaatta
     3481 atgaaatcag caaaatcatt tcagctgcag gtaacttcga tgttaaggag gaaagatctg
     3541 ccgcttcttt attgcagttg tccggtaatg ccagtgattt ttcatatgga cggaactcga
     3601 taactttaac agcatcagca taatttatta atttaaatga tagcaatctt actgggctgt
     3661 gccacataag attgctattt tttttggagt cataatggat tcttgtcata aaattgatta
     3721 tgggttatac gccctggaga ttttagccca ataccataac gtctctgtta acccggaaga
     3781 aattaaacat agatttgaca cagacgggac tggtctggga ttaacgtcat ggttgcttgc
     3841 tgcgaaatct ttagaactaa aggtaaaaca ggtaaaaaaa acaattgacc gattaaactt
     3901 tatttctctg cccgcattag tctggagaga ggatggacgt cattttattc tgactaaagt
     3961 cagtaaagaa gcaaacagat atcttatttt tgatctggag cagcgaaatc cccgtgttct
     4021 cgaacagtct gagtttgagg cgttatatca ggggcatatt attcttatcg cttcccgttc
     4081 ttctgttgcc gggaaactgg cgaaatttga ctttacctgg tttattcctg ccattataaa
     4141 atacaggaga atatttattg aaacccttgt tgtgtctgtt tttttacaat tatttgcatt
     4201 aataaccccc cttttttttc aggtggttat ggacaaagta ttagtgcaca ggggattttc
     4261 aactcttaat gttattactg tcgcattatc tgttgtggtg gtgtttgaga ttatactcag
     4321 cggtttaaga acttacattt ttgcacatag tacaagtcgg attgatgttg agttgggtgc
     4381 caaactcttc cggcatttac tggcgctacc gatctcttat tttgagagtc gtcgtgttgg
     4441 tgatactgtt gccagggtaa gagaattaga ccagatccgt aattttctga caggacaggc
     4501 attaacatct gttctggact tattattttc attcatattt tttgcggtaa tgtggtatta
     4561 cagtccaaag cttactctgg tgatcttatt ttcgctgcct tgttatgctg catggtctgt
     4621 ttttattagc cccattttgc gacgtcgcct tgatgataag ttttcacgga atgcggataa
     4681 tcaatctttc ctggtggaat cagtcacggc gattaacact ataaaagcta tggcagtctc
     4741 acctcagatg acgaacatat gggacaaaca attggcagga tatgttgctg caggcttcaa
     4801 agtgacagta ttagcaacca ttggtcaaca aggaatacag ttaatacaaa agactgttat
     4861 gatcatcaac ctgtggttgg gagcacacct ggttatttcc ggggatttaa gtattggtca
     4921 gttaattgct tttaatatgc ttgctggtca gattgttgca ccggttattc gccttgcaca
     4981 aatctggcag gatttccagc aggttggtat atcagttacc cgccttggtg atgtgcttaa
     5041 ctctccaact gaaagttatc atgggaaact ggcattaccg gaaattaatg gtgatatcac
     5101 ttttcgtaat atccggtttc gctataagcc tgactctccg gttattttag ataatatcaa
     5161 tctcagtatt aagcaggggg aggttattgg tattgtcgga cgttctggtt caggaaaaag
     5221 cacattaact aaattaattc aacgttttta tattcctgaa aatggccagg tcttaattga
     5281 tggacatgat cttgcgttgg ccgatcctaa ctggttacgt cgtcaggtgg gggttgtgtt
     5341 gcaggacaat gtgctgctta atcgcagtat tattgataat atctcactgg ctaatcctgg
     5401 tatgtccgtc gaaaaagtta tttatgcagc gaaattagca ggcgctcatg attttatttc
     5461 tgaattgcgt gaggggtata acaccattgt cggggaacag ggggcaggat tatccggagg
     5521 tcaacgtcaa cgcatcgcaa ttgcaagggc gctggtgaac aaccctaaaa tacttatttt
     5581 tgatgaagca accagtgctc tggattatga gtcggagcat atcatcatgc gcaatatgca
     5641 caaaatatgt aagggcagaa cggttataat cattgctcat cgtctgtcta cagtaaaaaa
     5701 tgcagaccgc attattgtca tggaaaaagg gaaaattgtt gaacagggta aacataagga
     5761 actgctttct gaaccggaaa gtttatacag ttacttatat cagttacagt cagactaaca
     5821 gaaagaacag aagaatatga aaacatggtt aatggggttc agcgagttcc tgttgcgcta
     5881 taaacttgtc tggagtgaaa catggaaaat ccggaagcaa ttagatactc cggtacgtga
     5941 aaaggacgaa aatgaattct tacccgctca tctggaatta attgaaacgc cggtatccag
     6001 acggccgcgt ctggttgctt attttattat ggggtttctg gttattgctg tcattttatc
     6061 tgttttaggt caggtggaaa ttgttgccac tgcaaatggg aaattaacac taagtgggcg
     6121 tagcaaagaa attaaaccta ttgaaaactc aatagttaaa gaaattatcg taaaagaagg
     6181 agagtcagtc cggaaagggg atgtgttatt aaagcttaca gcactgggag ctgaagctga
     6241 tacgttaaaa acacagtcat cactgttaca gaccaggctg gaacaaactc ggtatcaaat
     6301 tctgagcagg tcaattgaat taaataaact acctgaactg aagcttcctg atgagcctta
     6361 ttttcagaat gtatctgaag aggaagtact gcgtttaact tctttgataa aagaacagtt
     6421 ttccacatgg caaaatcaga agtatcaaaa agaactgaat ctggataaga aaagagcaga
     6481 gcgattaaca atacttgccc gtataaaccg ttatgaaaat ttatcgagag ttgaaaaaag
     6541 ccgtctggat gatttcagga gtttattgca taaacaggca attgcaaaac atgctgtact
     6601 tgagcaggag aataaatatg tcgaggcagc aaatgaatta cgggtttata aatcgcaact
     6661 ggagcaaatt gagagtgaga tattgtctgc aaaagaagaa tatcagcttg tcacgcagct
     6721 ttttaaaaat gaaattttag acaagctaag acaaacaaca gacagcattg agttattaac
     6781 tctggagtta gagaaaaatg aagagcgtca acaggcttca gtaatcaggg cccctgtttc
     6841 gggaaaagtt cagcaactga aggttcatac tgaaggtggg gttgttacaa cagcggaaac
     6901 actgatggtc atcgttccgg aagatgacac gctggaggtt actgctctgg tacaaaataa
     6961 agatattggt tttattaacg tcgggcagaa tgccatcatt aaagtggagg cctttcctta
     7021 cacccgatat ggttatctgg tgggtaaggt gaaaaatata aatttagatg caatagaaga
     7081 ccagaaactg ggactcgttt ttaatgtcat tgtttctgtt gaagagaatg atttgtcaac
     7141 cgggaataag cacattccat taagctcggg tatggctgtc actgcagaaa taaagactgg
     7201 aatgcgaagc gtaatcagct atcttcttag tcctctggaa gagtctgtaa cagaaagttt
     7261 acatgagcgt taagtctcag agccgcggta tccggctcat atcttctcct gtcatgtgtg
     7321 actgcactgt tttgtggcaa gccagtactg actatccact ggaattcggt agtcattaac
     7381 tatggcattg atgtccttta taactgtctt acagggcgta taacacatcc cgcgacggaa
     7441 gcttatcaca gaggaagagg taagcttcag aatggaaagt atatgagaag actgtgattg
     7501 tttaaaaata cacaatccgc tgtgaggtgg agtcagggga ggtactggcc cgcagaagcg
     7561 gagaatggtt gatgtatgag gcgagtgtga catttttcat gaaagtaaaa caggtgataa
     7621 cctcctttgg ttagggggta gtggtcaact aattttggcc acacgacctg actgttcgtg
     7681 gaacagctgc tctgattcat ttggcgttaa gccaccgtta taccagtggg gccggatggc
     7741 actgtaatag cctgtgatgt agcggattat cgcgctctga gcctcgttga agctgttata
     7801 gcccttcgtc ggcacccatt cggtcttcag gctccggaag aaccgctcca tcggggcatt
     7861 atcccagcag ttaccccgtc gactcatact ctgctttatc cgacagcgcc acagagcctg
     7921 ccggtactgg cggctggtat agtggctgcc ctggacgccc ttctaagagt caagccgtta
     7981 tcgggatgct gttgatctgc ctgttttgat tacgcagtaa tgtataaact tcgcgtgaga
     8041 tatagcgctt caggcagcgt attgcttcca tttttgtatg tccttccgct actcgtctgg
     8101 cgacatattc cttcgtttta tcgtcagttc gcaaacgtcc gatggcaatg atgtgaagtg
     8161 cactatttgc agcacgatct ccaccccggt taagtcgata acgattcgtt tttcctgaag
     8221 atacgggaac agggctgaca ccacacagtg ccgcaaaacc tgattctgat cttaatcgtt
     8281 ggggattgtc tcctgccgtg atcagcaact gcgaagcgct ttcgtatcca atagcattac
     8341 gtttaatcag ttcaggcgcc agctcatcga caattgccgc aatcatgaca tccaaatcag
     8401 cgatttcgtc atgtaactcg agatagcgtc gggcaaggga ctttaatgaa atacgataaa
     8461 cgttggtaac attgcggtat tcactggcat caggccgcca ggatcccaga gtcctgatga
     8521 gctgcatgcg cgtcatattt ctgagctgtt cacgtaattc atccggggca gagataatat
     8581 tggaatggat aatctggaga gcgactctgc gggctgatat tgctgttttt cggcaagttt
     8641 ttaatacccg cagagactca atcatgccat tgcgcgtttt gggtgtgacg gttcttattc
     8701 cggagaatgc ggcgtgagcg gcacattcag catcaatcgt gtcactttta ccccgtttgc
     8761 gtcgctccat ccgatctggc gcagtcacct caagaacgtc taacccggca ttctgtaaat
     8821 agcgaagcag acctgatcca taggtgccag tacactcaac accaattcgc tttaatgccc
     8881 caaacgaagt catccatgcc agcatctgcc ggtaaccttg ccgtgttgtg gagaaaaact
     8941 gggtccccag aactttattg ttctgatcta cgacagcggc aacgtgcaga tctttatgtg
     9001 tatccacgcc acccacaaca gcggtttccg taactttacc tgccataaca gagctcctgt
     9061 gaggaaacag agttgaatct ccagacagat aacccggaca ggacagtaac gagacaaacc
     9121 gtcaggccct tcttgagtca cgcgcatcgg tgaggagacg cctcgcatga aggcgcttcc
     9181 ggcaaccgac aggtccaggg caggacacaa agggtcgatc gctgtgtgag tcatgatgcg
     9241 ggaaggtctt cactgcatca gtaatcacac cagtctggtt aaacagcaaa cagagtgatg
     9301 ataaaaactg ataccggcat tattactgtc actgtggaac atcacgcctg ttggcttacc
     9361 ccgaagctcc caggccatct gcaacgcttt gaccgtgagg gcagagtccg gcgacgtcga
     9421 catcgcccag cccacaggtt tgcgggcgaa cagatccagc actactgcca gataagccca
     9481 gcattttccc gtccagatat aggtcacatc gccgcaccag acctgatccg gcgcggtaac
     9541 cgcgaactgc cggtcgagat ggttcggtat ttcaacgtgt tcgttcccgc cgcgtttgta
     9601 tttatgcacc ggtacctggc aactggagat atccagctct ttcatcagct taccggccag
     9661 ccatcgcccg agtctgacgc ccttagcgct gaccatcgtg gcgatacttc tcgcgccagc
     9721 agagccaccg ctggcgttcc agacttcact gacgagactc cgtttgacgg cacgctcagc
     9781 atgaggcccc ctgctgtttt tacgaatgta gcgataactg cttcggtgaa caccgaacag
     9841 ccggcaaaat ggcgctaccg ggtagtgcgc cctaagactg tctattatcg tgaactgttc
     9901 agggagtccg acatcaagag cgcggtagcc ttttttagga tttcattctc catttcaagg
     9961 cgttgtattc gttttctcat ttccctgagt tcagtctgct caggtgtcag aggcagcccc
    10021 gggggcgttt tcccctggcg ctccatacgt aacgatttta cccagcggtt gatggcggag
    10081 aggctgacgt tcatcgcctt agccgcttcg ccgtgagtgt agttctgatc caggaccagt
    10141 tttgccgctt cgactttaaa ttcagcagta aatgctttgc tcattggttc acctgtaaga
    10201 tgttgaggtg agcatatcac ctctactcag gtggccaaat tcagtgtgcc actacatatg
    10261 tagtcaggcg tgataaattt catatggaac aggtatgcgt tttatttgtg ataacagtta
    10321 atgaggtgtt tccatacacc ctgaagttac ctgtaatatt agcgggggat ttgaatgatg
    10381 ttgcgtggtc tgcgaccact cgtttattca tgcaaataag tggactgctg gatccacggt
    10441 aagagtacag cgagggccgt attgacgggg atgtgttatt cagcgggcag tactatgcgc
    10501 cacggaagca gttcgctgac acggttgacc ggccagtcag ctatgacgcc agacacatgg
    10561 cgaaggtagt tttctggatc ctcgtcgttc agtttgcacg tcccgatcag gctgtacagt
    10621 agcactcccc gctcaccacc atgctcagag ctgcgtatta ccgtgaagga gatcggtgag
    10681 taaccctctg tgtcggcaca ttatagccgt cacatcggat aactgttatc cttctgttct
    10741 gatatattct gggaggtgat gtttcactcc tgataagagc attactaatt acagctgctt
    10801 ttcggataac attcgggcag ttttctttaa ttctgaagtc tgaaagagat atcagtaatt
    10861 gtattacttt taaacattgt cagtatttat ttttccaaat cgttcacgtt tctcataatc
    10921 ttcccgacag tcaccatcac aaaacaatcc tgtcttaaca ggttctccgc agttatagca
    10981 gaatcctgtt tcagggagtc tattccggat acgatttttt agtctgatgc tcatgctgaa
    11041 ttgttcattt tcataagcaa tatctgcact atctgccata aacgatcctc tgaggatacc
    11101 acatctttat aacccaccac cgaaatatta caaagtaata ctcattgtat aatctttaac
    11161 ggcaggggca ggataattgt atcctgcccc tgctttaatt ctcagcacag gtgttgacta
    11221 aagagcgtga aactttgcta ttatgtcttc gtaagattca cggacggtta tacttgagcc
    11281 tgattctgtg aagtaaacaa cagcagaagc atcgttgcct ttttcaatgt atgaaacatt
    11341 ccagtcatgg atagccactg cgggctgacc attatcccga cggtgcgtct taatgaatcg
    11401 cggaagtaat tctgcaatat cgttaaaaac accatttacg gtatgagtga taccaccaac
    11461 gcaatgtaga tgagttgact ccggggtatc attgtctgct tctgcaaaga gtatagctgt
    11521 cttgctaatt gtaacaggcg cctgtgagcg ggataattcg agagaaataa acccggattc
    11581 tgccataaaa actccagttt gtgatgttat atcatttcat gtgttttttt gtttaacaga
    11641 aaatctttct catgtcaata ttatgagtgg tttctgcctg taaactaact ttcatctccc
    11701 gaaatctgtg agagctaagg atcgtggcgt ttatgatcag aagcaggttg taaatgacag
    11761 gagcattgcg ccgggatgcg tatgttctcc ccggacagac agtctcagtt cgtagaatct
    11821 ataaattact gctactgatg ctgccgggga aaggcgtaac gaaaaaacag cctccgttac
    11881 cggacagcaa ggaggctgaa tggagtttac aggatttgct tttgtataat gtctggccat
    11941 gcagtaaaac cggacaggtt ttattatcat gtgaggtatt ctgacataaa atgctggatt
    12001 tttattttgt gacgaatgct gcaaaattga atctgcactc tgatgtaact tttatctgtt
    12061 tcagtgaagc atgcccacag attgagttat taagttgtgg aagaacagct ctgccccgcc
    12121 tgcttctctc ctttcaaaaa ccagtatgtt gccatgcccc gtcttaatgg tgagcgctga
    12181 accatacctt ctttttccag taataacagg taatagcggg cctggtaatc cgttaccgcc
    12241 agcgcctctg ctatttctgc agttttcctt ccattatgcc tgttcagaaa ttccagtatt
    12301 tcattcttca tatattcact catctcactg taacaaagtt tcttcgaata ataaaaatca
    12361 tgctctctgt tatcaacgga aaggtatttt tattctctgt gtttgcttta tttgtgaaat
    12421 ttagtgaatt tgctttttgt tggctttatt tgatgtgtgt cacattttgc gtgttatttt
    12481 tctgtgaaaa gaaagtccgt aaaaatgcat ttagacgatc ttt
//
LOCUS       DADTFG010000140        12506 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.141, whole genome shotgun sequence.
ACCESSION   DADTFG010000140 DADTFG010000000
VERSION     DADTFG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.141"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            36..401
                     /locus_tag="J1138_003726"
                     /pseudo
     CDS             36..401
                     /locus_tag="J1138_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            585..1151
                     /gene="slp"
                     /locus_tag="J1138_003727"
     CDS             585..1151
                     /gene="slp"
                     /locus_tag="J1138_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBA8455470.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLNSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1304..1834
                     /locus_tag="J1138_003728"
     CDS             1304..1834
                     /locus_tag="J1138_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8455471.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(1891..2919)
                     /gene="chuS"
                     /locus_tag="J1138_003729"
     CDS             complement(1891..2919)
                     /gene="chuS"
                     /locus_tag="J1138_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HBA8455472.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGSFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYAADNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(2968..4950)
                     /locus_tag="J1138_003730"
     CDS             complement(2968..4950)
                     /locus_tag="J1138_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HBA8455473.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            5634..6548
                     /locus_tag="J1138_003731"
     CDS             5634..6548
                     /locus_tag="J1138_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter substrate-binding protein"
                     /protein_id="HBA8455474.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            6568..7905
                     /locus_tag="J1138_003732"
     CDS             6568..7905
                     /locus_tag="J1138_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HBA8455475.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            7918..8412
                     /gene="hutX"
                     /locus_tag="J1138_003733"
     CDS             7918..8412
                     /gene="hutX"
                     /locus_tag="J1138_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HBA8455476.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            8412..9035
                     /gene="chuY"
                     /locus_tag="J1138_003734"
     CDS             8412..9035
                     /gene="chuY"
                     /locus_tag="J1138_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HBA8455477.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            9120..10076
                     /locus_tag="J1138_003735"
     CDS             9120..10076
                     /locus_tag="J1138_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA8455478.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            10073..10843
                     /locus_tag="J1138_003736"
     CDS             10073..10843
                     /locus_tag="J1138_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HBA8455479.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVTA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(10895..11542)
                     /locus_tag="J1138_003737"
     CDS             complement(10895..11542)
                     /locus_tag="J1138_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBA8455480.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11606..11932)
                     /gene="hdeB"
                     /locus_tag="J1138_003738"
     CDS             complement(11606..11932)
                     /gene="hdeB"
                     /locus_tag="J1138_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBA8455481.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(12048..12380)
                     /gene="hdeA"
                     /locus_tag="J1138_003739"
     CDS             complement(12048..12380)
                     /gene="hdeA"
                     /locus_tag="J1138_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBA8455482.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTKDKKASFKDKVKGE
                     WDKIKKDM"
BASE COUNT         3300 a         3080 c         2883 g         3243 t
ORIGIN      
        1 tcttagcata aaacaaatga tgaataagga atgttatgtc aattgacttt accccaggtg
       61 taataaatac atataacggt gatatttata actgcacaac aaataccgat aatgttaaaa
      121 cgccagacac accgaagtgg ccttgtgata accgggaaga acaacacccc attaattccc
      181 cattctctgg agaatggata cacctctgaa caatttgatt tagcgcaaca acgccaacaa
      241 attaatgcgt gccatacgaa caccacatat acaaatgcag actactcaaa gttgaggctc
      301 aacttgtaag tcttattaat aacattgaaa cgataagctc gacaccactt ccgcaaaaga
      361 cacaatcaat cattagacac gaaaagatta aaaacctctg aatatagatt atggaacaat
      421 ggatactcat gccacgccca aaaattaact gagctcaact aaacagaaga gatataaaca
      481 tcagacaggt ttacgttact atcaggcata tcgcctcaga atcagatgaa aactataaag
      541 aaatatctat tatggtttta atatttgttg ataaggatag taacatgaac atgacaaaag
      601 gtgcactcat cctcagcctt tcatttttgc ttgccgcatg tagttcaatt ccgcaaaata
      661 tcaaaggcaa taaccaacct gatattcaaa aaagttttgt tgctgttcat aaccagccgg
      721 ggttatatgt tggtcaacaa gcgcgctttg gtgggaaggt tatcaacgtt atcaatggca
      781 aaacggatac gttgttagaa atcgctgtat taccgttgaa tagctatgcg aagcctgata
      841 ttgaagccaa ctatcagggc cgactgctcg ccagacaaag cggcttcctt gatccagtga
      901 actatcgtaa tcactttgtt accatcctcg gtaccattca gggtgaacaa cctggcttta
      961 tcaataaagt cccgtataac ttcctggaag tgaatatgca gggcatccag gtgtggcatt
     1021 tgagagaagt ggttaatacc acctataact tgtgggatta cggctatggt gcattctggc
     1081 cggaaccggg ctggggggct ccttactaca ccaatgcggt gagtcaggta acacctgagc
     1141 tggtcaaata acaccaccga aagatgcaga tgctttctca gcatctgcat catgcattac
     1201 atcaaattaa tacacagtaa gctaactatt attataagcc ctgtcctgtt aattaccttt
     1261 ggcaaactga ttataaagtt aatgtccgta ccaggagtcg gttatgtttc ttataattac
     1321 cagggatacg atgtttttca ccgcgatgaa aaacattctg agtaaaggta atgtcgttca
     1381 tatacagaac gaagaagaga tcgacgtaat gttgcatcag aatgccttcg tcattattga
     1441 tacattaatg aataatgtat ttcattctaa ttttctcact caaattgaac gattaaaacc
     1501 tgtccatgta attattttct ccccctttaa tattaaacgc tgcctgggga aagtaccggt
     1561 gacctttgtt ccgcgaacta tcgccatcat cgactttgtc gcactcatca atggcagtta
     1621 ctgctctgtg cctgaagcgg atgtgtccct ctcgcgcaag cagcatcagg ttctgagctg
     1681 tattgcgaat caaatgacaa cggaagatat tctggagaaa ctgaaaatat cgctaaaaac
     1741 gttctactgc cataaacaca atatcatgat gatcctcaat cttaagcgga tcaatgagct
     1801 ggtacgccat cagcatatta attatctggt gtgaacgatt gaacagtata aagaggccca
     1861 gcaacagcca gacctcccgt taattatacg ttatgcagta acgccttccg gtatcaacga
     1921 agcaatttgc ttacgccatt gcgcttgctc ctgttcacct tctgtacgtt gaccataaag
     1981 ttgcgctatc tgcgtaccat catgggcaaa caattccagg ctggttacgt ggccatcgct
     2041 ggtcggttta cgggtaaccc aggcttcagc aatgctctct tctaatagat gaagggtaaa
     2101 cgtcgggttg aaaatattca gccaaccttt cattggcacc actttttcta ccacaccggt
     2161 gaaaatctgt acgcagccac ggttgccaac aaacaccatg atttcattgc catcctgctg
     2221 tgcagattca agaatttgcg ccaacgcact gttggatact ttgcaggcca aatcgtctgc
     2281 caccagattg aacgcctgtt ggcgcgtcag gttgtggcgc ttgagcaacg taaaaaactg
     2341 atgaacgtcg gtcatcgccc gccactcttg ctcgaccaca ctggcatcgg ctcgcgtttg
     2401 aacaactggc gcatcaacgg cttttaactc aagcggcgta ttctcatcgg tgataaaccg
     2461 tgccagaagc tcactccatg ccgccatatc ggtattgtcg gcggcataaa cttttagtaa
     2521 tgcatcgccc tgatgatcaa agaactgaat actctggcgt tcgccacgag ccgtgttttc
     2581 tttgatgtgg aaaacactgg cccattgatt gagaaacaga cgtaaatcca gcgcgcgcgg
     2641 attgaggatc aatccggcat gtccgttcaa atgctggttt gtgaacgagc caacttgctc
     2701 atggactgca tattcattac gacaaataca tttggtttca ccaacgcttt ccagcgccgc
     2761 cagaatttca cgaatatcgc cgcgcatccg ccacgcgtcg tgcgtgacgc gcgcaaatgc
     2821 cagttctgct tcgctgatat tcattaaacc tgcgatgtca cgcgcgtact ttccgggatt
     2881 ttgttctttt aactcaagcc agcgtgtgta gtggttcatt gtctctcttc cttccagata
     2941 aatgccccga aatatcgggg cagatgatta ccattgataa ctcacgaaaa tttttccgtt
     3001 acgaccatcc tgtgggatgc cttgcggcga ccagtactct ttatcgaagg cgttgcccaa
     3061 taccagagta gtggtcatac ctttgagcgc ctgttgtcct tgataactga cgtagaaatc
     3121 attcacacca tagccaggtt gtttgctgta gctgctgctg atatgtgttg agcgatcggc
     3181 aaacgtaccg acccaaccaa cagagaagcc actgtgagcg atcggaatat tcagggtact
     3241 ggtaacggta tccgggttaa tgctggagat atattccccg gtatcggtgt ctttgccgcg
     3301 ggtacggtta taggccacat caaggctaaa cagatcagtg gtatatttcg tcatcacatc
     3361 ccagccccag attttggcgt tcgggacgtt atacgacata gttgtcgccg ccgcgaaatc
     3421 gacggtcgtg gagatataat ctttcgcttt ggtatcaaag tagctggctt taaattccag
     3481 agcatcattg gacaacatca ggtcatcaaa acgcagccca aaaccgtact cctgagtttc
     3541 gttagtttcc ggacgtaagt tcggatttgg cacccaatag ttggtataga agcgaccaat
     3601 cgagaagtgt ttagaatcgt tatacatttc gcccatcgtc ggggcgcgga atgcctgagc
     3661 atatgagcca aataacatca gccagttggt cgggttgata gtcatccccg cacgagatga
     3721 ccatttgtcg gcatcaacat ctttgtagcc gtcgctgcta ccgcgataac tgtcatagcg
     3781 ggttccgcca agcagggtaa tcggcagatc gcgtaaggtg atctcatctt gtagccagcc
     3841 agagctaaaa tcgatttttg cttgcgggaa gcccgtcgtc gcgccacccg gatgttgttc
     3901 ctgacgataa tactcaccgc catatgtcag taagtgagaa gcgaaactgt cggcaaatag
     3961 agtggaacgg ttctccagcc ttgctccttt tgttatctgt tcacgatact cgccggaact
     4021 ccccgtgttt tgcgcattaa tacggacttc cgaccagtat atttttgcat ctgcatttaa
     4081 ccagtcgtta ccctgcgggg cgagtttata agaaagctgc gcatcgcgtt gaattgttga
     4141 acgatcgacc atcgggttgc tgctatcaga agcttcaacg gtctgcggat tttttggttc
     4201 acgcgcgtcg ttgttgtagt aacgcactaa accgctcaga gactgggctg aatcaatttg
     4261 ccaggtccct ttcgccagca tgttattaat ggattcgtca ttcggcgcgg tttcaccatt
     4321 gctctggcgt aaatcacccc gatcgcgact ggaccaagcc acaataccat ccagattttc
     4381 agttcgccca aacgcgctcg cgcctaatcc caggctatgg tcccccgtgc caccagtacc
     4441 aaagacacga aaaccactgc tttgtccttc ctgcaataaa tcttttgcat cgaccgtatc
     4501 gtaggagatc actccaccca gcgcgccact gccatacagt aatgctgaag gtccgcgaac
     4561 aatctcaaca cgcttgatca gcgccggatc gagaaaagtg ccattcaggt gtccggtatc
     4621 cgttccctgg cgaacaccat cgacaagaac cagcacgccg cgatgatcat agccacgcat
     4681 atttacatcc tgaccgttgg ttcgtccggt accatcaaga gtaattccag gaacatgacg
     4741 cagcaaatca gtggctgaag tagcagtttg attttcagga gcggaagtgt cgataacgct
     4801 gaccatcata ggcgcttcga aggaactacg tgcattcccc gttgccgtaa cggtcatggt
     4861 ttcagtagca aaagcaaacg ttggcaaggt ggcagaaaca gccaaagcca acaaactcaa
     4921 acgcaacgag gtaaattgcg gacgtgacat agcgattctc catgaggaaa ataaaaaatg
     4981 ctggctgcct gggttaatac cggggtggct ggcgaaaaat aaatttattt acaattcaaa
     5041 tgattagata tcaccacggg aagctattag cgtatatatc ccgtaaaatt gccgaatatt
     5101 tctgataatt cagcaaaagc acgccctctt tgtcaggcaa agtagtacgc cacgtctgaa
     5161 gtgaagagtg ctgtgcgttt ccccttacaa aagatatgat tttgttatcc atgatgtcat
     5221 attttgttaa actcaaacgt gataatgaga attattatcg atcaattttt tatgatcaag
     5281 ctttttttaa ccgccattaa cacaataaac agggggttat gaaatctatc cagcatccag
     5341 aaaaccatcc tgtgtttatg gctatccccc cacaacctca tcacaaaacg ttgccgttaa
     5401 cagtgataat tttctcttct ctttgctctg caagccgcga tatctccggg ttctcagctt
     5461 tttcccgcgc tgaaatttaa tcggtaacaa gaacattagc tgacgaagca tcttgataaa
     5521 aatcaaaaat aaatacgctt gtaacgccgt gcgtattgaa tcgacggttg tatttcatat
     5581 ataactcaaa tgataatcat gatcattctc aatttgataa gagttctcac attatgccaa
     5641 ggatcatcac caggccgttt cttttttccc cactaacgtt atgtatttca gccgtggcct
     5701 ctgccgcgaa gaccatggta aaacgaaaaa aattgtttac ggcattactg gctcttagct
     5761 ggacttttag cgtaacggct gccgaacgta tcgtggtcgc aggaggatcg ctgacggagc
     5821 taatctacgc gatgggcgct ggcaaacgcg tggttggtgt cgatgaaacg acatcctatc
     5881 caccagaaac cgccaaactg ccgcatattg gttactggaa acaactcagc agcgaaggca
     5941 ttttgtcact tcgcccggat agcgtaatta cctggcagga tgcaggaccg caaattgtgc
     6001 tcgaccaact gcgggcgcaa aaagtcaacg tcgtcactct gccgcgtgta ccagccaccc
     6061 ttgagcagat gtacgccaac attcgccaac tggcaaaaac gttacaggtt cctgaacaag
     6121 gcgaggcgct ggtgacacag atcaaccaac gcctggagcg agtacagcaa aacgtggcgg
     6181 ccaaaaaagc cccggttaaa gccatgttta ttctctccgc aggcggaagt gcgccacagg
     6241 ttgccggtaa aggcagcgtc gcggacgcca ttctttcgct tgccggagca gaaaacgtcg
     6301 ctacccacca gcagtacaaa agctacagcg cggaatcgct gattgcggct aaccctgaag
     6361 tgattgtcgt cacctcacaa atggtcgatg gcgatattaa tcggctacgt tctattgccg
     6421 gaattaccca cactgccgca tggaaaaacc agcgcattat caccgttgat caaaacctga
     6481 ttctgggaat gggtccgcgt attgctgatg tcgttgagtc tttacaccag cagctttggc
     6541 cgcaataaat ggatttaaat cagacgaatg aacacaaata acaccctgga tctcacgcca
     6601 cattttgctt tagacggtga tcagccattt aaagacaggc gcgccatgat gccgtttcgt
     6661 ggggccattc cggttgccaa agagcaactg gcacagacct ggcaagagat gatcaatcaa
     6721 acggtatcac cacgtaagcg actggtttac ctacacattc ctttttgtgc gacacactgc
     6781 acgttttgtg gtttttatca gaaccgtttt aatgaagatg cgtgtgctca ttataccgac
     6841 gccctgattc gtgaaataga aatggaagca gacagcgtat tgcatcagtc ggcccccatc
     6901 cacgcggtct atttcggtgg cggtacgcct tccgcgcttt cagcacacga tttggccaga
     6961 atcatcacca cattacgaga aaagctgcca ctggcccctg attgtgaaat cactatcgaa
     7021 ggccgggtac tgaattttga cgctgagcga atcgatgctt gccttgatgc tggagcaaac
     7081 cgcttctcaa ttggtattca gtcgtttaac agcaaaatcc gcaagaaaat ggcccgcacc
     7141 tcggatggcc caaccgctat tgcgtttatg gaaagcctgg tcaaacgcga ccgtgccgcg
     7201 gtggtctgtg acctgctgtt tggtctgccg ggtcaggatg cgcaaacctg gggagaagat
     7261 ctggctattg cccgcgatat cggtctcgac ggcgtcgatc tctatgcgct caatgtcctg
     7321 cccaatacac cgctgggcaa agccgtggaa aatgggcgta ctaccgtgcc ctctccggca
     7381 gaacgtcgcg atctttacct gcaagggtgt gattttatgg acgatgccgg ttggcgctgc
     7441 atcagtaaca gccactgggg ccgtaccaca cgcgaacgca atctctataa cctgctgatt
     7501 aaacaaggtg ccgattgtct ggcctttggt tccggagccg gcggttcgat taatggttac
     7561 tcctggatga acgaacgcaa tcttcagacc tggcatgaat ccgtcgcagc aggcaaaaaa
     7621 ccgctgatga tgatcatgcg taacgccgaa cgtaacgcgc agtggcgtca taccttgcag
     7681 tcgggcattg aaacggcgcg tgtaccgctg gacgaactta cgccacatgc cgaaaaactc
     7741 gcgccgttac tggcccagtg gcaccaaaaa ggcttaagcc gcgatgcgtc aacgtgtctg
     7801 cggttgacca atgaaggtcg tttctgggca agcaacattt tgcagtctct taatgaactg
     7861 attcaggtac ttaatgcgcc agcaattgcg cttgaaaaac cataacagga gtgatttatg
     7921 agccatgtct cgttacagga atttttgaaa acggagccag acggtacgct ggaggcgatc
     7981 gccgaacaat acaacaccac gctgctggag gtagtgaaaa atctgccctc tccgactgtt
     8041 gtgtcaggtg ataaattcga tactgtctgg gataccgtct gcgaatgggg caacgtcacc
     8101 acgttagtac atactaccga tgtaatactc gaatttagcg gcgaactgcc ttcgggtttc
     8161 catcgccacg gctatttcaa cctacgcgga aagcacggca tgtccgggca tatcaaagca
     8221 gaaaactgca cgcatattgc cttgatcgaa cgtaaattta tgggcatgga caccgcctct
     8281 attctctttt tcaataagga aggcagcgca atgctgaaaa tcttcctcgg tagggatgat
     8341 catcgtcagc tcctgagcga acaagtcagc gccttccatg ctctggcagc atcgttaaag
     8401 gaacacgcct gatgacaccg tggctactct ttggcgcagg cggaaaaggt gtgggtgcca
     8461 gaacgcttga actggcgctg gcggaacaac gtccggtcgt tgctgtcatt cgtcatgccg
     8521 atgccgccac gaaactggcg caacaaggcg tacaggtttt tacaggcgac gcctgtgatg
     8581 ccagcgtagt cgcggctgct tgccgcgcag ctggcccgga tgcgcttatc atctcaacaa
     8641 tgggcggagc tcaggattat ctggcgcaca gaacagtgat tgatgaggcc gagaaggctg
     8701 gcatcagtcg catgattctg gtgacctctt tgggatgcgg cgatagctgg ccgtttttat
     8761 cagagcgggc aaaggctgcg tttggtcagg cggtacggga aaaaacgctg gcggaaagct
     8821 ggttgcaaac cagccagctt gattatgcca ttctccgccc cggcggtctg cttgatggtg
     8881 cggcaacggg caaagcacaa cggatacaaa atcaggagtg tcatggtttt gttaaccgtg
     8941 ctgatgtcgc cgcacatatc catgaactgg caaatgcgcc agcacttaac cagcaggtct
     9001 acagtctgat tgaaccggac ctgaaaccgg cgtgatcccc cggcgcggta tgtccgcgcc
     9061 gtaatgtcgt aacggagttt tgcgtgctca aggattcact ctcttccgcc agggtcttta
     9121 tggggctatc actattattg ctagctctgg tgctgtttgg tgccagtcag ggagcgttaa
     9181 agatcagttt tgatgccctt tttgatgagg aataccgcga tatctggctc aatattcgtc
     9241 taccacgggt tttgctggcg gtgctggtag gtgcagcgtt ggcaaccgca ggggtaatta
     9301 tgcaagggct ttttcgcaac ccaatggctg accctggatt acttggcgtc agtagcggtt
     9361 ctgcattaat ggttggcgtt gctattgtgc tgcccttctc cttcccagtg gtgctggtgc
     9421 tttatgagca aatggtgttt gccattgccg gaagtttagt ggtctgcacc atcatttttc
     9481 tcatcacgca gcgccatcgc gatggcagca tgatgcaatt attactcgcc ggtatcgcca
     9541 tcaatgccct gtgcggcgca gcgatcggca tcctgagcta tatcggcgat gagcagcagc
     9601 tacgacaact cacattgtgg atgatgggca atcttggaca ggcgcaatgg ccgacgttat
     9661 tggttgccag ttcattcatc ctaccggcca ttatcgcaac aacttgtctc gccggaacgc
     9721 tgaatttact gcagctcggt gatgaagaag cccactacct cggcgtgaac gtgaagcgta
     9781 aacgccagca attactgtta gtgagctcac tgctcgttgg tgctgccgta tcggtaagcg
     9841 gtattatcgg ctttattggc ctagtgatcc cgcatctgat tcgcatgact accggggcaa
     9901 atcaccgctg gctaatccct tgttccgccc tcgccggagc ctgtttattg ctgatggcag
     9961 acacgcttgc ccgcacgctg gtacagccag cagaaatgcc cgtgggatta ttaaccagcc
    10021 tgcttggtgg cccttatttt atgtggttga ttctgcgcaa ccggaggatc acatgatcag
    10081 cgcccagaat ctggtttata gccttcaggg gcggcgtctg actgataatg tctcgctgac
    10141 ttttcccggt ggggaaattg ttgccatcct cgggcctaat ggtgcgggaa aatctacgct
    10201 gcttcgccag ctaaccggct atcttcaacc tgatagcggc gaatgtcggt tgtttaataa
    10261 gccattaaat gaatggtcga ttactgaact ggcgaaacac cgggccgtga tgcgtcaaaa
    10321 tagccacatg gcgtttccat tcagcgtaca agaagttatc cagatggggc ggcatccgca
    10381 tcgtactggt aatcaggata atgaaacagc acaaataatg gcgttatgtg actgtcaggc
    10441 gttagcaaac cgcgactatc ggcaattatc cggtggtgaa caacagcgcg tgcaattagc
    10501 gcgcctgctg gtgcaactct gggaacctac tccctcaccg aaatggttat ttcttgatga
    10561 accaacttcg gcgctggata ttcaccatca gcagcacttg tttcgtttat tacgccagtt
    10621 agttcacgaa cggcagttca atgtttgctg cgtattgcac gatctaaatc ttgccgcgcg
    10681 ttatgcagac cgtgttgtat taatgcaaaa aggaaaggtt accgcaaatg gtaagccgca
    10741 ggacgtattg acacagcagg cgctaacaat gctctacggc gcggatatta ccgtacttaa
    10801 agatcctgcc aaccattcac cactgatcgt cctcgatcat taaaaccaac aggagtaccg
    10861 acctggtact cctgcgaata aataactata cagattagct aattgaaacg cctttcaccc
    10921 ctgccatacc ttttaataat cgcaacagat cttctatcga cgttgtggca tgaagctgga
    10981 tatcaatggc gaccacttca tgatcttcat tttcctgcaa cgaaactaaa tccgttttta
    11041 ttttttgctg cttaaaccag tcgagcattg ataccgtatt accgttcact aatgtcagct
    11101 gtaaatggta atttttgttc atcaggcgga aggttaattg atggaagact tccagcacca
    11161 gcaaggtcat cactgaacca taaataccca gttcgtacat accgctgcca ataaccatac
    11221 caatggcggc ggtcacccag atatccgctg ccgtcgtcag acctacaata ttttggttac
    11281 gaacgagaat gttacctgca ccgataaacc cgacgcccgt cacaacctga gcagcaatac
    11341 ggctgggatc cagtccgacg tgacctaaag acagcacgtc agcaaaacca tatttcgaaa
    11401 caatcataaa caaggcgctt cccatgccaa ttaatacatg tgtgcgtaac cctgctcctt
    11461 tgccgcgcat ttgcctttcc atgccaatag cgccacaggc aattaccgca agtataagtc
    11521 gtataataaa ttctgctgtc atataaaaat gcaaaaagga gcagcaagat ggctcaactt
    11581 gctactcctt tttacttgta ccttattaat tcggcaagtc attagatgct tgatttttga
    11641 aggtatacaa atttttctgc gggtttttct tgcagtattc gatcacttta ggaatttgag
    11701 tgagatcggt ttcatttaaa gtaacggtat cgccaccttt atatactgtt tcttcatgca
    11761 gcatccacca cgcaactggt gtcattgctt ttggattcag atcaataaat tcctggcagg
    11821 tcatatcttt agcggattca ttggctgcca gcgcagattg tgcgttcacc agtgacaaag
    11881 ccgctacagc gcccataaaa ataaacgctt tacggagaga tgaaatgttc attttgtaac
    11941 ccattcaata tagagattat atacaaacag gaggaatagc tgcttttaaa gaagatattc
    12001 cggcctgttc aatatgcaag gaaatacgat gtaacgcatt cccggaatta catatctttc
    12061 ttaattttgt cccattcgcc tttaacttta tctttaaagc tggctttttt atccttagta
    12121 caagcctgaa cgatagctgg ggttacggtt gcaataccct gaacatctaa aaccgcatct
    12181 tctggtttat ctttgttgtt cagtgcttca gcaaaaccaa ctgcagttgg ctggaaggat
    12241 tcgtccacag ccaggaaatc ttcacaggtc caggagttga ccggtttttt gttatcagct
    12301 gctttttgtg catccgctgc attgctcact actggcagaa gaagcagacc accaagaata
    12361 acgcctaata cttttttcat cgtaatatcc tcaactataa agtgaaagag ccgtcacgaa
    12421 tcaatttcga cactgaggtt ataacctggt tttctgtata tgtcatgttg atggaaaata
    12481 tcaaaatcag atatttttat ttcaat
//
LOCUS       DADTFG010000141        12351 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.142, whole genome shotgun sequence.
ACCESSION   DADTFG010000141 DADTFG010000000
VERSION     DADTFG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.142"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            67..450
                     /locus_tag="J1138_003740"
     CDS             67..450
                     /locus_tag="J1138_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455483.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVLAMFKQK"
     gene            complement(514..957)
                     /locus_tag="J1138_003741"
     CDS             complement(514..957)
                     /locus_tag="J1138_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA8455484.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTERSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="J1138_003742"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="J1138_003742"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBA8455485.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2312..3913
                     /gene="aceB"
                     /locus_tag="J1138_003743"
     CDS             2312..3913
                     /gene="aceB"
                     /locus_tag="J1138_003743"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBA8455486.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKISGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYSNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGETIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSHGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3943..5247
                     /gene="aceA"
                     /locus_tag="J1138_003744"
     CDS             3943..5247
                     /gene="aceA"
                     /locus_tag="J1138_003744"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBA8455487.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5296..7020
                     /gene="aceK"
                     /locus_tag="J1138_003745"
     CDS             5296..7020
                     /gene="aceK"
                     /locus_tag="J1138_003745"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBA8455488.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(7037..7861)
                     /gene="iclR"
                     /locus_tag="J1138_003746"
     CDS             complement(7037..7861)
                     /gene="iclR"
                     /locus_tag="J1138_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBA8455489.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            8061..11744
                     /gene="metH"
                     /locus_tag="J1138_003747"
     CDS             8061..11744
                     /gene="metH"
                     /locus_tag="J1138_003747"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBA8455490.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
     gene            11804..12124
                     /locus_tag="J1138_003748"
     CDS             11804..12124
                     /locus_tag="J1138_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455491.1"
                     /translation="MIKIDEIDFFAFEGCSESEVTELLGKKPTGYNDWIINIKTGVEW
                     VFYDGRYWLDFRKSKEGIRCISCSILYHDKSKGRIDVLFSEGIADRGLKNTGGVLIQK
                     RPQL"
     gene            12167..>12351
                     /locus_tag="J1138_003749"
     CDS             12167..>12351
                     /locus_tag="J1138_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455492.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNGRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVA"
BASE COUNT         3034 a         3104 c         3283 g         2930 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcctgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt tccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaacccaa ccggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggctgtaca ggccgcgctc
     1561 aatatcctct atggtattcc taagcaaacc cgcaccgaca aactctctgg cgtttacgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattatac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgcatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccgggctaga cccggatgta ccgtataact atttcccgca caatgatccg
     1921 caaaatacac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa cacacctcag caagtgaata aattttattt atattgttat
     2161 cagtaagtta acatgtattt ttaattaaaa tggaaattgt ttttgatttt gcattttaaa
     2221 tgagtagtct tagttgtgct gaatgaaaag cgcacaacga tccttcgttc acagtgggga
     2281 agttttcgga tccatgacga ggagctgcac gatgactgaa caggcaacaa caatcgatga
     2341 actggctttt acaaggccgt atggcgagca ggagaagcaa attcttactg ccgaagcggt
     2401 agaatttctg actgagctgg tgacgcattt tacgccacaa cgcaataaac ttctggcagc
     2461 gcgtattcag cagcagcagg atatcgataa cggaacgttg cctgatttca tttcggaaac
     2521 agcttccatt cgtgatgctg actggaaaat tagcgggatt cctgcggact tagaagatcg
     2581 tcgcgtagag attaccggac ctgtagagcg caagatggtg atcaacgcgc tcaacgccaa
     2641 tgtgaaagtc tttatggccg atttcgaaga ttcactggca ccagactgga acaaagtgat
     2701 cgatgggcaa attaacctgc gcgatgcggt taacggcacc atcagctata gcaatgaagc
     2761 aggcaaaatt tatcagctca agcccaatcc cgcggttttg atttgtcggg tacgcggtct
     2821 gcacttgccg gaaaaacatg tcacctggcg tggtgagaca atccccggta gcctgtttga
     2881 ttttgcgctc tatttcttcc acaactatca ggcactgttg gcaaagggca gcggtcccta
     2941 tttctatctg ccgaaaaccc agtcctggca ggaagcggcc tggtggagcg aagtcttcag
     3001 ctatgcagaa gatcgcttta atctgccgcg cggcaccatc aaggcgacgt tgctgattga
     3061 aacgctgccc gccgtgttcc agatggatga aatccttcac gcgctgcgtg accatattgt
     3121 tggtctgaac tgcggtcgtt gggattacat cttcagttat atcaaaacat tgaaaaacta
     3181 tcccgatcgc gtcctgccag acagacaggc agtgacgatg gataaaccct tcctgaatgc
     3241 ttactcacgc ctgttgatta aaacctgcca taaacgcggt gcttttgcga tgggcggcat
     3301 ggcggcgttt attccaagca aagatgaaga gcgcaataac caggtgctcg acaaagtaaa
     3361 agcggataaa tcgctggaag ccaataacgg tcacgatggc acatggatcg ctcacccggg
     3421 ccttgctgat acggcaatgg cggtattcaa cgatattctc ggctcccgca aaaaccagct
     3481 tgaggtgatg cgcgaacaag acgcgccgat tactgccgat cagctgctgg caccttgtga
     3541 tggtgaacgc accgaagaag gtatgcgcgc caacattcgc gtggctgtgc agtacatcga
     3601 agcgtggatc tctggcaatg gctgtgtgcc gatttatggc ctgatggaag atgcggcgac
     3661 ggctgaaatt tcccgtacct caatctggca gtggatccat catcaaaaaa cgttgagcaa
     3721 tggcaaaccg gtgactaaag ccttgttccg ccagatgctg ggcgaagaga tgaaagtcat
     3781 tgccagcgaa ctgggcgaag aacgtttctc ccatgggcgt tttgacgatg ccgcacgctt
     3841 gatggaacag atcaccactt ccgatgagtt aattgatttc ctgaccctgc caggctaccg
     3901 cctgttagcg taaaccacca cataactatg gagcatctgc acatgaaaac ccgtacacaa
     3961 caaattgaag aattacagaa agagtggact caaccgcgtt gggaaggcat tactcgcccg
     4021 tacagcgcgg aagatgtggt gaaattacgc ggttcagtca atccggaatg caccctggcg
     4081 caactgggcg cggcgaaaat gtggcgtctg ctgcacggcg agtcgaaaaa aggctacatc
     4141 aacagcctcg gtgcactgac tggtggtcag gcgctgcaac aggcgaaagc gggtattgaa
     4201 gcagtctatc tgtcgggatg gcaggtagcg gcggacgcta acctggcggc cagcatgtat
     4261 ccggatcagt cgctctatcc ggcaaactcg gtgccagcgg tggtggagcg gatcaacaat
     4321 actttccgcc gtgcggatca gatccaatgg tccgcgggca ttgagccggg cgatccgcgc
     4381 tatgtcgatt acttcctgcc gatcgtcgcc gatgcggagg ccggttttgg cggtgtactg
     4441 aatgcctttg aactgatgaa agcgatgatt gaagccggtg cagcggcagt tcacttcgaa
     4501 gatcagctgg catcagtgaa gaaatgtggt cacatgggcg gcaaagtttt ggtgccaact
     4561 caggaagcta ttcagaaact ggtcgcggcg cgtctggcag ctgatgtgac gggcgttcca
     4621 accctgctgg ttgctcgtac cgatgccgat gcggcggatc tgattacctc cgattgcgac
     4681 ccatatgaca gcgaatttat tactggcgag cgtaccagtg aaggcttctt ccgtactcat
     4741 gcgggtattg agcaagcgat cagccgtggc ctggcgtatg cgccatatgc tgacctggta
     4801 tggtgtgaaa cctccacgcc ggacctggaa ctggcgcgtc gctttgcaca agccatccac
     4861 gcgaaatatc cgggcaaact gctggcttat aactgctcgc cgtcgttcaa ctggcagaaa
     4921 aacctcgacg acaaaactat tgccagcttc cagcagcagc tgtcggatat gggctacaag
     4981 ttccagttca tcaccctggc aggtatccac agcatgtggt tcaacatgtt tgacctggca
     5041 aacgcctatg cccagggcga gggtatgaag cactatgttg agaaagtgca gcagccggaa
     5101 tttgccgccg cgaaagatgg ctataccttc gtatctcacc agcaggaagt gggtacaggt
     5161 tacttcgata aagtgacgac cattattcag ggcggcacgt cgtcggttac cgcactgact
     5221 ggctccactg aagaatcgca gttctaacaa ccaacgcccc tttccgtaag gcgaggggcg
     5281 aattaaggag aggcgatgcc gcgtggcctg gaattactga ttgcacaaac cattttgcaa
     5341 ggtttcgacg ctcagtatgg tcgattcctc gaagtgactt ccggggcgca gcagcgtttc
     5401 gagcaagccg actggcatgc tgtccagcag gcgatgaaaa accgtattca tctttacgat
     5461 catcacgtgg gtctggtcgt ggagcaactg cgctgcatta ctaacggcca aagcacggac
     5521 gcggcatttt tactgcgcgt taaagagcat tacacccggc tgttgccaga ttacccgcgc
     5581 ttcgagattg cggagagctt tttcaactcc gtgtactgtc ggttatttga ccaccgctcg
     5641 cttactcccg agcggctttt tatctttagc tcccagccag agcgccgctt tcgtaccatt
     5701 ccacgcccac tggcgaaaga ctttcacccc gatcacggct gggaatctct gctgatgcgc
     5761 gttatcagcg atctgccgct gcgtctgcgt tggcagaata aaagccgtga cattcattac
     5821 atcgttcgcc atctgacgga aacgctgggg acagacaacc tcgcggaaag tcatttacag
     5881 gtggcgaacg aactgtttta ccgcaataag gcagcctggc tggtgggcaa actaataacg
     5941 ccttccggca cgttgccatt tttattgccg atccaccaga cggacgacgg cgagttattt
     6001 attgatacct gcctgacgac gaccgccgaa gcgagcattg tttttggctt tgcgcgttct
     6061 tattttatgg tttatgcacc gctgcccgca gcgctggtcg agtggctacg ggaaattctg
     6121 ccaggtaaaa ccaccgctga attgtatatg gctatcggct gccagaagca cgctaaaacc
     6181 gaaagctacc gcgaatatct cgtttatcta cagggctgta atgagcagtt cattgaagcg
     6241 ccgggtattc gtggaatggt gatgttggtg tttacgttgc cgggttttga tcgggtattt
     6301 aaagtcatca aagacaggtt cgcgccgcag aaagagatgt ctgccgctca cgttcgtgcc
     6361 tgctatcaac tggtgaaaga gcacgatcgc gtgggccgaa tggcggacac ccaggagttt
     6421 gaaaactttg tgctggagaa gcggcatatt tccccggcat taatggaatt actgctccag
     6481 gaagcagcgg aaaaaatcac cgatctcggc gaacaaattg tgattcgcca tctctatatc
     6541 gaacgacgga tggtgccgct taatatctgg cttgagcaag ttgaggggca gcaattgcgt
     6601 gatgccattg aagagtacgg caacgccatt cgccagcttg ccgctgccaa catcttccct
     6661 ggggatatgt tatttaaaaa ctttggcgtc acccgtcacg ggcgcgtggt gttttatgat
     6721 tacgacgaaa tttgctacat gacggaagtg aatttccgcg acatcccgct gccgcgctac
     6781 ccggaagacg aacttgccag cgaaccgtgg tacagcgtct cgccgggcga tgttttcccg
     6841 gaagagttcc gtcactggtt atgtgccgac ccgcgtattg gtccgctatt tgaagagatg
     6901 cacgccgact tgttccgcgc tgattactgg cgcgcactac aaaaccgcat acgtgaaggg
     6961 catgtggaag atgtttatgc gtatcggcgc aggcagcggt ttagcgttcg attcgtataa
     7021 atcagaggcg aaaaagtcag cgtattccac cgtacgccag cgtcacttcc ttcgccgctt
     7081 taatcaccat cgcgccaaac tcggtcacgc ggtcatcggt aatacgtgaa atcggtccgg
     7141 aaattgaaat tgcggcaaac ggttcgcggt gctcatcgaa aatacacgct gcaaggcaac
     7201 gcagccccag cgcatgttcc tcatcgtcaa atgaataacc ccgtttgcgc gtttgggcga
     7261 gatcttcttt taaatgcaca ggagacacca gcgtcgcgtg ggtataggca tgtaaccctt
     7321 tgcggtgcag cagcttcgtc acctgttctt cgctcagttg ggccagaaag gctttacccg
     7381 caccggaagc gtgcatcggc aatttaccgc cgataggcgc ggacatacgc atcaggtgag
     7441 tacactgtac ctggtcgata ataatcgctt cgtgattgct ttgatcaagc accgccatat
     7501 tgaccgtttc gccagactct tccattagat tgcgcaggat agggtgaaca atcgctaaca
     7561 aattacggct ctggagaaag ctgctaccga ccataaaggc gtgtgcgccg attgcccagt
     7621 gacccagttc gccgacctga cggacaaagc cctgttgctg catcgtggtt agcaggcggt
     7681 gggtcgtgga attgggtaac ccggcttgct gtgccagttc cgtgagtgcc acactgccat
     7741 tggattcggc aatccactcc agtaatttca ggccacgcgt taaagactga acctgtccag
     7801 tcgctggtgc ggtggcaacg gcgggttttc tgccgcgttt tgcgggaatg ggtgcgacca
     7861 tgacagtctc ctttttctgt atcgtggaaa tcattttcat ttttattatc agcgaatgca
     7921 atagttgctg aacagatccg atgagttaat gttgaacaaa tctcatgttg cgtggtggtc
     7981 gcttttacca cagatgcgtt tatgccagta tggtttgttg aatttttatt aaatctgggt
     8041 taagcgtgtc gggagcaagt gtgagcagca aagtggaaca actgcgtgcg cagttaaatg
     8101 aacgtattct ggtgctggac ggcggtatgg gcaccatgat ccagagctat cgactgaacg
     8161 aagccgattt tcgtggtgaa cgctttgccg actggccatg cgatctcaaa ggcaacaacg
     8221 acctgctggt gctcagtaaa ccggaagtga tcgccgctat ccacaacgcc tactttgaag
     8281 cgggcgcgga tatcatcgaa accaacacct tcaactccac gactattgcg atggcggatt
     8341 accaaatgga atccctgtcg gcggaaatca actttgcggc ggcgaaactg gcgcgcgcct
     8401 gtgctgacga gtggactgcc cgcacgccgg agaaaccgcg ctacgttgcc ggtgttctcg
     8461 gcccgaccaa ccgcacggcg tctatttctc cggacgtcaa cgatccggca ttccgcaaca
     8521 tcacttttga ccagctggtg gcggcttatc gagaatccac caaagcgctg gtggaaggtg
     8581 gcgcggacct gatcctgatt gaaaccgttt tcgacaccct taacgccaaa gcggcggtct
     8641 ttgcggtgaa aacggagttt gaagcgctgg gcgttgaact gccgattatg atctccggca
     8701 ccatcaccga cgcctccggg cgcacgctct ccgggcagac tactgaagcg ttttacaact
     8761 ccctgcgcca cgccgaagcg ctgacctttg gcctgaactg tgcgctgggg ccagacgaac
     8821 tgcgccagta cgtacaggaa ctgtcgcgta ttgcggaatg ctacgtcacc gcccacccga
     8881 acgccgggct gcccaacgcc tttggtgaat acgatctcga cgccgacacg atggcaaaac
     8941 agatacgtga atgggcagaa gcaggctttc tcaatatcgt cggcggctgc tgcggcacca
     9001 cgccacaaca tattgcagcg atgagtcgtg cagtagaagg attagcgccg cgcaaactac
     9061 cggaaattcc cgtagcctgc cgtttgtccg gcctggagcc gctgaacatt ggcgaagata
     9121 gcctgtttgt gaacgtgggt gaacgcacca acgtcaccgg ttccgctaag ttcaagcgac
     9181 tgatcaaaga agagaaatac agcgaggcgc tggatgtggc gcgtcagcag gtggaaaacg
     9241 gtgcgcagat tatcgatatc aacatggatg aagggatgct cgacgccgaa gcggcgatgg
     9301 tgcgttttct caatctgatt gccggtgaac cggatatcgc tcgcgtgccg attatgatcg
     9361 actcctcaaa atgggacgtt attgaaaaag gtctgaagtg tatccagggc aaaggcattg
     9421 ttaactctat ctcgatgaaa gagggtgtcg acgcctttat ccatcacgcg aaactgttgc
     9481 gtcgctacgg tgcggcagtc gtggtaatgg cttttgacga acagggccag gccgataccc
     9541 gcgcacggaa aatcgagatt tgccgtcggg cgtacaaaat cctcaccgaa gaggttggct
     9601 tcccggctga agatatcatc ttcgacccga acatcttcgc ggtcgcaact ggcattgaag
     9661 agcacaacaa ttacgcgcag gactttatcg gcgcgtgtga agacatcaaa cgtgaactgc
     9721 cgcacgcact gatttccggc ggcgtttcta acgtttcttt ctcattccgt ggcaacgatc
     9781 cggtgcgcga agccattcac gcggtgttcc tctactacgc tattcgcaat ggcatggata
     9841 tggggatcgt caacgccggg caactggcga tttacgacga cctgcctacc gagctacgcg
     9901 acgcggtgga ggatgtgatc ctcaaccgtc gcgacgatgg caccgagcgt ttactcgagc
     9961 ttgccgagaa atatcgcggc agcaaaaccg acgacaccgc taacacccag caggcggagt
    10021 ggcgctcgtg ggaagtgaat aaacgtctgg aatactcgct ggtcaaaggc attaccgagt
    10081 ttatcgagca ggataccgaa gaagcccgcc agcaggctac gcgtccgatt gaagtgattg
    10141 aaggcccgtt gatggacggc atgaacgtgg tcggcgacct gtttggcgag ggcaaaatgt
    10201 tcctgccgca ggtggtgaaa tcggcgcgcg taatgaagca ggcggtggcg tatctcgaac
    10261 cgtttattga agccagcaaa gagcagggta aaaccaacgg caagatggtg atcgccaccg
    10321 tgaaaggcga tgtccacgac atcggtaaaa atatcgttgg cgtggtgctg caatgtaaca
    10381 actacgaaat tgtcgatctc ggcgtgatgg tgcctgcgga aaaaattctc cgtaccgcta
    10441 aagaagtgaa tgctgatttg attggccttt cagggctgat cacgccgtcg ctggacgaga
    10501 tggttaacgt ggcgaaagag atggagcgtc agggcttcac cattccgcta ttgattggcg
    10561 gcgcgaccac ctcaaaagcg cacacggcgg tgaaaatcga gcagaactac agcggcccga
    10621 cggtgtatgt gcagaacgca tcgcgcaccg ttggtgtggt ggcggcgctg ctttccgaca
    10681 ctcagcgcga tgatttcgtt gctcgtaccc gcaaggagta cgaaaccgtg cgtattcagc
    10741 acggacgaaa gaaaccacgc acaccaccgg tcacgctgga agcggcgcgt gataacgact
    10801 tcgcttttga ctggcaggct tacacgccgc cggtggcgca ccgtctcggt gtgcaggaag
    10861 tcgaagccag catcgaaacg ctgcgtaatt acatcgactg gacgccgttc tttatgacct
    10921 ggtcgctggc cgggaagtat ccgcgcattc tggaagatga agtggtaggt gttgaggcgc
    10981 agcggctgtt taaagacgcc aacgacatgc tggataaatt aagcgccgaa aaaatgctga
    11041 acccgcgtgg tgtggtgggc ctgttcccgg caaaccgtgt gggcgatgac attgaaatct
    11101 accgtgacga aacgcgtacc catgtgatta atgtcagcca ccatctacgc caacagaccg
    11161 aaaaaaccgg cttcgctaac tactgtctcg ctgacttcgt tgcgccgaag gtttccggca
    11221 aagcagatta catcggcgca tttgccgtga ctggcgggct ggaagaggac gcactagctg
    11281 atgcctttga agcacagcat gatgattaca acaaaatcat ggtgaaagcg cttgccgacc
    11341 gtctggcgga agcctttgcg gagtatctcc atgagcgtgt gcgtaaagtc tactggggct
    11401 atgcgccgaa cgagaacctc agcaacgaag agctgatccg cgaaaactac cagggcatcc
    11461 gtccggcacc gggctaccct gcctgcccgg aacatacgga aaaagccaca atctgggagc
    11521 tgctggaagt ggaaaaacat acaggtatga aactcacgga atccttcgcc atgtggccgg
    11581 gtgcatcggt ttcgggttgg tacttcagtc atccagacag caagtattac gcggtggcgc
    11641 aaattcagcg cgatcaggtt gaagactatg cccgccgtaa aggaatgagc gttaccgaag
    11701 ttgagcgttg gttggcaccg aatctggggt atgatgccga gtaagtgata acaccggggt
    11761 atttgccccg gtttttaatg tcataaataa tggattattg aacatgataa aaattgatga
    11821 aatagatttt tttgctttcg aaggatgttc agagtccgaa gttactgaac tattaggaaa
    11881 aaaaccaaca ggttataatg attggataat taatataaaa acaggtgtgg agtgggtatt
    11941 ttatgatggt cgttattggt tagattttcg taaatccaaa gagggtataa ggtgtatctc
    12001 atgcagtatt ctgtatcacg ataaaagtaa agggcgtatc gatgtcttgt ttagtgaagg
    12061 tatagctgac agaggtttaa aaaatacggg tggagtgtta atccaaaaac gcccgcaatt
    12121 ataataattc ctagtaatat ttgttttcgt aaaaaggatt tactttatgc aacgtcaata
    12181 taaaaaatac attcttcttt tttggggtgt tatggatgtt atcgcgatag ccacatatct
    12241 gttctactcg cttggtaatg gtcgtatccc tttttattcc gatatcgttc attcactttc
    12301 tttacttaag gatatcggtg ttgaaggcgg tttttacgct tatgcagtcg c
//
LOCUS       DADTFG010000142        12118 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.143, whole genome shotgun sequence.
ACCESSION   DADTFG010000142 DADTFG010000000
VERSION     DADTFG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.143"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..1820)
                     /gene="selB"
                     /locus_tag="J1138_003750"
     CDS             complement(<1..1820)
                     /gene="selB"
                     /locus_tag="J1138_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBA8455493.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSAEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
     gene            complement(1817..3208)
                     /gene="selA"
                     /locus_tag="J1138_003751"
     CDS             complement(1817..3208)
                     /gene="selA"
                     /locus_tag="J1138_003751"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBA8455494.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3306..3914)
                     /locus_tag="J1138_003752"
     CDS             complement(3306..3914)
                     /locus_tag="J1138_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBA8455495.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3985..5121)
                     /locus_tag="J1138_003753"
     CDS             complement(3985..5121)
                     /locus_tag="J1138_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA8455496.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5124..5486)
                     /locus_tag="J1138_003754"
     CDS             complement(5124..5486)
                     /locus_tag="J1138_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBA8455497.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVAPQGDVPDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6023..7936
                     /locus_tag="J1138_003755"
     CDS             6023..7936
                     /locus_tag="J1138_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBA8455498.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8255..9403
                     /gene="mtlD"
                     /locus_tag="J1138_003756"
     CDS             8255..9403
                     /gene="mtlD"
                     /locus_tag="J1138_003756"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBA8455499.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9403..9990
                     /gene="mtlR"
                     /locus_tag="J1138_003757"
     CDS             9403..9990
                     /gene="mtlR"
                     /locus_tag="J1138_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HBA8455500.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(10001..10210)
                     /locus_tag="J1138_003758"
     CDS             complement(10001..10210)
                     /locus_tag="J1138_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455501.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            10494..10856
                     /locus_tag="J1138_003759"
     CDS             10494..10856
                     /locus_tag="J1138_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBA8455502.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYVELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            11415..12098
                     /locus_tag="J1138_003760"
     CDS             11415..12098
                     /locus_tag="J1138_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBA8455503.1"
                     /translation="MNLKKTLLSVLMILQLCLLVGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGKILSIDAVENGSVIRISLVNLLSVPVSNMGFYATWGGEKPTDINAL
                     AKWQQLLFSTSMNSSLKLLPGQWQDINLTLKGVSPNNLKYLKLAINMANIQFDRLQPA
                     ESPQRKNKK"
BASE COUNT         3036 a         3204 c         3014 g         2864 t
ORIGIN      
        1 tcgcgcaata aatgatcatt tccacgacga cgcgtaaagc caatacggtc aaaatattcc
       61 agaatctgaa ttgccagctt gcggcctacg cctaagcgat cgcggaaatc cgccgcgcag
      121 gttgaaccgc attcctgatc gagatcgcgg atcatattgg caaattcgac aatccgatcg
      181 ttacggtaat aacgatcttt gacgatcgca gtaattatcc cttgttgcgc cgcctggcgt
      241 agcgtcaggc gcattgcctg ctcgtcggtt cccgtctctt ttgccaggtc acgcacccac
      301 catggctcgt caccaaacag cggctctgct ttttgccaga tggcctgctg ctctgcactg
      361 aagcccgctt tatgatccgg cagatgcagc cagccgtgat ggctgtggat gtcgccgctt
      421 tcgcgcatct tttcaatcag caacagtacc agcgcttcat cttccattgg caacgccata
      481 cgtcgcagac gttcgcgccc aggaccaggt tcatcgcgat gttgctcatg ataagtcgct
      541 aatgtatcga gaattttccg ctgccagcgg gcggcaactg gcgcattcaa caagctgtaa
      601 ccagcctgaa tatagccagg ctgttgcagc agttcgcgca tcccttcgcc gttgagctgc
      661 cgtgcccagg cgaaatccgc aaggttaacc gcgccgcgtt ccagatgaac agataacgca
      721 tcggcatcgc tctgcgcccg tgcaagagac gccagccatt gcagatattc cggtttacgc
      781 ttaccgcgac gcggcgggtt aagcatcacc acacgcgccc ccgccagcgt gttgcgggcc
      841 gagatatcgc gcagcactaa gcgatcgtta tccgccagcc acagcggcgt gtcgaacacc
      901 agctcggcaa ggttatcttc cagcagcgaa acgcgtcccg taacgtggct ggcggcgtgg
      961 tgaatatgca gcggctgcca ctgggtcagc ggtgtatggg tttgaagttc gacgatcacc
     1021 cgtgtgaacg gctccggcgg cacatcggca agcagccagt cgccacggtt aatctgctct
     1081 ttttccgcat cacccgcgat gttaagcgcg atacgctgcc cggcatgggc agtttctgtt
     1141 ggctgatttt gcgcatgcag cgcacgcacg cgcatcggtt tattcacacc agtcagccag
     1201 agtgaatcgc ctactttcac ttccccgctt aacgccgtac cggtgacgac cagcccggca
     1261 ccttttacgg taaatgcgcg gtcgatcgcg aggcggaaac tatgttggct ggcgtgttca
     1321 cgttctggta actgaagcag atgctcgcgc agggcatcaa ttccccgacc ttcggttgct
     1381 gcggtgataa acagttttgc ctcagcaaaa ccgtattccc gcagcacctc ctttacctgg
     1441 cgttcaacct catcaacacg cgcttcgtcc acgcgatcgg ctttggtcag cgccactgtc
     1501 agcatcgggt taccggtcag ctgcaaaatc gccagatgct cacgggtctg tgccatcaca
     1561 ccgtcatcgc acgccaccac caacagcgcg tgatcgatac caccaacgcc cgccagcatg
     1621 ttggaaagaa acttttcatg accgggtacg tcgataaaac caggcacgcg accatccggc
     1681 tgcggccagt aggcatagcc gagatcgatg gtcatgccgc gctttttttc ttccggcaga
     1741 cggtcggcat ttacgcccgt aatcgcctgc aacaaggttg ttttgccgtg gtcaacgtgt
     1801 ccggcagtcg caataatcat ttcaacaaca tctccaaaaa ccgttgctca tcttcaaggc
     1861 agcgtaaatc cagccacaat cgtccgtcat aaatacgacc aatcaccggc actggcaatt
     1921 cacgccagcg ggcggctaat gactcaaggt ggctaccgcg tccatcatgg ggggtaaacg
     1981 ttaatgccgc gctcggcagg cgatcaaccg gcagcgaacc actgccgatc tgcgaaagac
     2041 atggcataac ctgtaccgca aactccgcgc cgtaatgtgc ggcaagggga gcctgtaaac
     2101 gttgtgcctg gatttgaatg acctctgcgc tgcgggtaag caggcgcagg gtcggtaatt
     2161 tttcactcag agcttcaggg tgtaaataaa gacgcaacgt ggcttccagc gccgcgaggg
     2221 tcattttatc cgcgcgtaat gcacgcttca gcgggtggct ttgcaggcgg gcgatcatct
     2281 cttttttacc aacaataatt cctgcctgcg gcccgcctaa cagcttgtcg ccggagaagc
     2341 tcaccagact gacgcccgcc gcaatcaact cctgcggcat tggctctttc ggcaaaccgt
     2401 actggctaag atcgaccagc gagccactgc ctaaatcagt caccactgga acatctagtt
     2461 ctttgccgag cgccaccagt tccgcttcat ctatcgcttt ggtgaacccc tgaatgctgt
     2521 agttactggt atgtactttc atcaacagtg cggtattttc attcaccgcc tgacgataat
     2581 cattcgcgtg cgtgcggttg gtggtcccta cttcgtgtag ggtgcatcct gcctgacgca
     2641 taacatcggg aatacgaaac gcgccgccaa tctccaccag ttcgccgcga gaaaccacca
     2701 cctctttccc gctggcagtg gccgccaaca tcaataacac cgccgccgca ttgttattaa
     2761 cgatacaggc atcttccgcc cccgtaatac ggcacagcag ctgcgccagc gcccgatcgc
     2821 gatgtccgcg tccggcgtcg tccagatcgt actcgagggt cactggcgaa cgcatagcct
     2881 tcgtaacggc ttccaccgcg gcttccgcct gtaaagctcg cccaaggttg gtatgcagca
     2941 cggttcctgt caggttgatt accggacgca gcgcgctttg cgcctctttc gtcaaccggg
     3001 catcgacttc ttgcgcccag ttttcacacc acgcgggcag cgtctggctg tcacgaatca
     3061 cttctcgcgc ttcgtcaagc atctgacgca gcaattccac cacgcgggtg tgaccataag
     3121 tatcacgcaa agaaaggaag gagctatcgc gcaataagcg atcaatagcc ggaagttgac
     3181 tatagaggga acgcgtttcg gttgtcatag gaaacctggc tgatcaaggc cctctcacac
     3241 ggagaagggc gtttaacata accacggatt gtaacgtgag atgggtcagg aggacatatc
     3301 gcgcatcaag cctttggcgg ttcggtgcga gcaaaacttt cgcggctaaa caggttttcg
     3361 accagtttga ctaagtgtgg gcggtcgaca caccagcctg gcgcaacgcg gcggaaattg
     3421 agatatccga cggcacaggc aatggcgata gtcgccagat tgaccgtatc ggttttgagc
     3481 gtgccatcga ccagatatcc ttccagcaca tccagactgc ggttgatttt ctcacgctga
     3541 cgtaacaatt catcttcaga ctgctgcgcc gccggacgcg cctgttcacg caccgatacc
     3601 agcccggcat ccataatgcc atccgccagt gcctcaattt tgcgcacccg caacgactca
     3661 agcggatcgc gcggcaacat cgccggagca acattcatta attcaatata ttcagcgatg
     3721 atcggtgaat caaaccagca ttcgccctct tcggtcacca gtaccggtac ttttcctaac
     3781 gggttaaatt gcgccacgcc gttatccgcg ttatagggca gttcattaat aaattcgaac
     3841 gttatgccct tttctaacaa cagaatagaa agtttgcgta caaacgggct ggtgtagcta
     3901 ccgacgagtt tcatgccgag tcctttgtgc gaggaaaaat atcagtatgg ctcaggctcg
     3961 cgcaaaacac aggtgataca agtctcaatg atccaaataa agataatgca tccaactggt
     4021 cattcttagc agcactttcc gcatcaccga aacatggctg aaatggtcgg agtaaactgc
     4081 cacctgggcg tagatgccgt cgggtaaggc atcgatatca gcgttagggt ccagttcaat
     4141 ggttcccagc acaccgtccg tgccgggcac gaccgttaat gattgcaata ccccctgcgc
     4201 ctgataagaa ccgcctggca caacaggtaa aatactggtc agtttgccgt gaaacacctg
     4261 cccaggtagc gcgttaaaca ccacttccgc atcatcgccg ggtttcagac gtaacagcga
     4321 gttttgccga aattgggcga caatttgccg tttttgctcg gggatgaaga ccatcaccgg
     4381 acgcagcggc aaggcagcgg cgtatgtacc tgggcggatc agtacctgag tgacgtagcc
     4441 attgctcggt gcgcgaatga cggtctgctc aaggttatat tttgcttcag taagttgcgc
     4501 tcttaagctc acaatctgcg attgctcgcc gttaaccata ctgtcgagct ggctctggat
     4561 ctgcgcctgc tccgccaccg agcctttcac cagcgcatcc tgcgcgagga aattttgccg
     4621 cgcatcgtcg atgtcacgtt ccgagaacgg attcaccgcc gcctggctgc ctttcagata
     4681 acgttgataa tttttaaaca gacggtcgcg ctccgctgaa acctgggtgg tgttggcctg
     4741 cgcttcagtg agctgcgcgc gcagcgtctt tatattatgc gtcgccgtca tcaggtcagc
     4801 ctgaagcctg tcaactcgcg cctggtaacg aaccgggtcg agcttaaaaa gcacctcgcc
     4861 cttctgaata agctgattat ttttgtcggt gacttcagta acaattcccg tcacctgtgg
     4921 cgtgatgggg atcgctatca ctgccttttg cgcggtaaaa gtgtaagggt ggttgtagtt
     4981 catcaacaaa atcaaaccac tcaccagaaa cacgcctccc agcgccgccg tcgccagcgt
     5041 ccactgattc accggaatgc ggaagatttt aaagaccgcc cacgccagcg ccacgtaagt
     5101 taaaacaatc aatagatcca taattagatc tccgggaacg tggaatagtc agtctttttc
     5161 tcggcggcga gttggtgctc cagccgggaa atccggtcag aaagtgcggc gatttccggg
     5221 tctggcacgt cgccttgcgg cgcaacatgt gactgcatgc cccagccgcg ctccggttga
     5281 tagagcgtcg cccagatcca caggaacggc cagataacat gcagagtaaa caggctcacc
     5341 cagcccgccg tatgaatagc gtcggcatgg ggatggttgc gctttttggc aatcagataa
     5401 gggatgtcat gtatcgcgat gattccataa aaaatcacca gaaagacgaa gatcagcact
     5461 cccaacgcga aatagtttag aaacatatct gcctcggatt cacgtttatc agtgttgttt
     5521 tttggctggc agccagaagg gagtcaggct gatattttga caataatccg ggttcgcgat
     5581 tctcgccata acaccaaaga ataattttaa gaggtgatga gttgcttagt tacataacga
     5641 tttcatgacg aaggcataac atgctgtaga tcacatcagg tgaacgccgt aagaaaatat
     5701 cttgtgattc agatcacaaa gattcaacaa accatcaaaa taaaaatgtg acataactca
     5761 catttaaatg ccatttttag cgaaaatcgc cgccttgttg cttttttaca caagcatttt
     5821 gtgatgagca tcacgtcaat tacctctcta ccccctacat ttatgtgact gatatcacac
     5881 aaaaggccgc cgactggaca gttaaccgat tcagtgccag atttcgcagt atctacaagg
     5941 tccggctacc tctgccgcca cattaacaaa aaacctcggg cttccagcct gcgcgacagc
     6001 aaacataaga aggggtgttt ttatgtcatc cgatattaag atcaaagtgc aaagctttgg
     6061 tcgtttcctc agcaacatgg tgatgccaaa tatcggcgca tttatcgcgt ggggtatcat
     6121 caccgcgtta tttattccaa cagggtggtt accgaacgaa acgctggcga agctggtcgg
     6181 gccgatgatc acttatctcc tgccgctgct gatcggttat accggtggta agctggttgg
     6241 cggcgaacgt ggcggcgtag tcggtgccat caccaccatg ggcgttatcg tcggcgcaga
     6301 catgccgatg ttcctcggtt ctatgattgc aggtccgctg ggcggctggt gcattaagca
     6361 cttcgaccgc tgggtagacg gtaagatcaa atccggtttt gagatgctgg tgaataactt
     6421 ctccgcaggc atcatcggga tgatcctcgc tattctggca tttctcggca ttggcccggt
     6481 tgttgaagcc ctgtctaaaa tgctggcggc gggcgttaac ttcatggttg tccatgacat
     6541 gctgccgctg gcgtctatct ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat
     6601 taaccacggt atcttctcgc cgctgggtat tcagcagtcc catgaactgg gtaaatccat
     6661 cttcttcctg attgaagcta acccaggtcc gggtatgggc gtgctgctgg cgtacatgtt
     6721 ctttggtcgt ggtagcgcta aacagtctgc gggcggtgcg gcaatcatcc acttcctggg
     6781 cggtatccac gaaatctact tcccgtatgt gctgatgaat ccgcgtctga ttctggccgt
     6841 tatcctcggt ggtatgactg gcgtgttcac gctgactatc ctgggcggtg gtctggtttc
     6901 tccggcatct ccgggttcta tccttgctgt actggcgatg acaccaaaag gtgcatactt
     6961 cgctaacatc gcgggtgtgt gtgcggcgat ggctgtttcc ttcgttgtct ctgctatttt
     7021 gctgaaaacc agcaaagtga aagaagaaga tgatattgaa gctgctgcac gtcgcgttca
     7081 ggaaatgaaa gctgagtcta aaggcgcgtc tccgctgtct gcgggcgatg tgactaacga
     7141 cctgagccac gtacgtaaaa tcatcgttgc ctgtgatgcc ggtatgggtt ccagtgcgat
     7201 gggtgcaggc gtgctgcgta agaaaattca ggatgcaggt ctgtcgcaga tttcggtcac
     7261 taacagcgcg atcaacaacc tgccgccaga tgtggacctg gtcatcactc accgtgacct
     7321 gaccgaacgc gctatgcgcc aggttccgca ggcacagcat atttcgctga ccaacttcct
     7381 cgacagcggc ctgtacacca gcctgaccga acgtctggtt gctgcccagc gccatactga
     7441 aaacgaagtg aaagtgaaag acagtctgaa agacagcttt gacgattcca gtgctaatct
     7501 gttcaaactg ggcgcggaga acatcttcct cggtcgcaaa gcggccacca aagaagaagc
     7561 gattcgtttt gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca
     7621 ggcgatgttg gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc
     7681 acacggtacg gttgaagcga aagatcgcgt actgaaaacg ggcgtcgtgt tctgccagta
     7741 cccggaaggc gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat
     7801 tgctgcccgt aacaacgagc acattcaggt gatcaccagc ctgaccaacg cactggatga
     7861 tgagtccgtt atcgagcgtc tggcgcacac caccagcgtg gatgaagtgc tggaactgct
     7921 ggcaggtcgt aagtaatcca ttcccaccct ctccgcatga agaaggtggg gctaattgcc
     7981 tgatgcgctt cgcttatcag gcctacaaca taaagcacaa tttgttttgt cggtcggata
     8041 aggtgtttac gccgcatccg gcagtcggta cacaatgcct gatgcgacgc tgacgcgtct
     8101 tatcaggcct acaggtcagt gccaaacacg taggccggat aaggcgttta cgccgcatcc
     8161 ggcaaaaagc ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc
     8221 ctctcgggta aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg
     8281 taatatcggt cgtggcttta tcggtaaact gctggcggac gcgggtatcc aactgacgtt
     8341 tgccgatgta aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca
     8401 tgtggtcggc gaaaccgagc aggtggatac cgtttctggc gtcgatgccg tcagcagcat
     8461 tggcgatgat gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc
     8521 tgtcgtgctg gaacgtattg ctccggccat cgccaaaggg ctggtgaaac gtaaagaaca
     8581 aggtaatgaa tccccactga acatcatcgc ctgtgaaaac atggtccgcg gcaccacgca
     8641 gctgaaaggc catgtgatga acgccctgcc ggaagatgcc aaagcgtggg tagaagaaca
     8701 cgttggcttt gttgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa
     8761 cgatccgctg gaagtgacgg tagaaacctt cagcgaatgg attgtcgata aaacgcagtt
     8821 caaaggcacg ctgccaaaca tccctggcat ggaattaacc gacaatctga tggcatttgt
     8881 cgaacgtaaa ctcttcaccc tgaatacagg tcatgctata accgcgtacc tcggaaaact
     8941 ggccggtcat cagaccattc gtgacgcaat tctcgacgag aaaatccgcg cggtggtaaa
     9001 aggggcaatg gaagaaagcg gcgcggtatt gatcaagcgc tacggctttg atgctgacaa
     9061 acatgcggca tacatccaga aaatcctcgg tcgtttcgag aacccgtacc tgaaagatga
     9121 tgtagaacgc gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa
     9181 gccactgctt ggaaccctgg aatatggtct gccacataaa aacctgattg aaggtattgc
     9241 cgctgcaatg cacttccgca gtgaagatga cccgcaggct caggaactgg cagcgctgat
     9301 tgccgacaaa ggcccacaag ccgcgctggc acagatttcc ggtcttgatg ccaacagcga
     9361 ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag
     9421 gataccctgc gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag
     9481 gcctttgaaa accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg
     9541 atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc
     9601 aaagacgatt acgcggtgaa gtatgctgta gaaccgttac tcgacggcga tggtccgctg
     9661 ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa
     9721 tacgaagatg cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag
     9781 tacgctttta ccgacgacga aatacttgga ccctttggtg aactgcattg cgtggcggcg
     9841 ttaccaccgc cgccacagtt tgaacctgca gactccagtt tgtatgcaat gcaaattcag
     9901 cgttaccaac aggctgtgcg atcaacaatg gtcctttcac tgacagagct gatttccaaa
     9961 atcagcttaa aaaaagcctt tcaaaagtaa gtactttcgc ttacttctcc tctacctgtt
    10021 tcggacgata aagctgacga taatactcca gacgttgtag atataacggc acgctttcat
    10081 caggtattcc ggacggaatc gcgttacggg gaggtagttt tttcagatac tcccggaacg
    10141 cctggctgga tgccatgaaa tctacggctt tatcgataag aagcggaacg ttttcaccta
    10201 atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt
    10261 agtttagatc ccggtataaa tgacattaag gaaaacgtgc tcaatcctca ataatcacct
    10321 gcttctgttg actatctttt ttctttaaat ttcttaacta atcaatgtat tgggtgattt
    10381 tttaacgcat aaatggttac aatctggaac tattacaatg ccatcagttg tgaacaaagc
    10441 acctggtcgc gcatactaga ggctataaat ttatctctat cagaagtcat cacatgaaag
    10501 aagtcgaaaa aaacgaaatc aaacgtctca gcgatcgtct ggacgccatc cgtcaccagc
    10561 aggccgatct atcgctggtt gaagccgcag acaaatatgt cgaactggaa aaagagaaag
    10621 ccacgctgga agcagaaatt gctcgcctgc gcgaagttca cagccagaaa ctcagcaaag
    10681 aagcgcagaa actgatgaag atgccgttcc agcgtgcaat caccaaaaaa gagcaggctg
    10741 atatgggcaa actgaaaaaa agtgttcgcg gactggtggt tgtgcaccca atgaccgcgc
    10801 tgggccgtga aatgggcctg caagagatga cagggttctc aaagactgcg ttttaagttc
    10861 ctttctcgtc gctgtaatca cagcctctca ccatttactc gctcctgctg gcacatgcag
    10921 gagcctgttt cagagatcga atctttataa cgttcttaac attatatatt caactccacc
    10981 tacacttctg agcgatcgta gcaggctaaa ttctgaaaat caggtacaca ttaaagaatg
    11041 atgtatcaat atcaacaatg aattgccgca tatcaatgct tgaaacataa ttaattattt
    11101 aaatttttca atcagcttcg caaattccac aataaaacat cactcaactg aatatttatc
    11161 tttccctgcc ctgtgccttt atccggtgaa acatttgaca tcgattgaca ccgatcatta
    11221 aaatccgcgc gattaatatt attggctaca aactgaaatc gtttgacatt taataacata
    11281 ttcgcacaac caattaatac aaaaactctc tgacaaattt attttgcgga tatgccccac
    11341 tccaatcatt cataaaaaaa atgattgtgc cgtaattatc aactgtatcg ggattatgaa
    11401 aaggaattca tcctatgaac ctaaagaaaa cactgttaag cgtgttaatg atattgcaac
    11461 tttgcttatt ggtagggtgt gactatattg aaaaagcgag taaggtcgac gatctcgtta
    11521 cacagcaaga gttacaaaaa agcaaaattg aggcgcttga aaaacaacaa gaactcgaca
    11581 agcgcaagat agaacacttt gaaaaacaac aaactaccat cataaacagt acaaaaacac
    11641 tcgctggtgt ggtgaaggca gttaaagaca aacaggacga atttgtcttt acagaattta
    11701 acccggcaca aacccaatac ttcattttaa ataacggttc tgttggtttg gcagggaaaa
    11761 tactgtctat tgacgcagta gaaaacggca gtgttattcg tatttcactg gttaacttat
    11821 taagtgttcc cgtatcaaat atgggtttct acgcgacatg ggggggagaa aaacccaccg
    11881 acatcaacgc attagcaaaa tggcagcaat tgctatttag tacctcaatg aactcctccc
    11941 tgaaattatt accaggtcaa tggcaagaca ttaatttgac gctaaaaggt gtctcgccca
    12001 acaacctcaa atatctgaaa ttagccatca acatggcaaa tattcagttc gaccgtcttc
    12061 aacctgctga atctccacag cggaaaaaca aaaaataaat gcagcttctg cgcttata
//
LOCUS       DADTFG010000143        12091 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.144, whole genome shotgun sequence.
ACCESSION   DADTFG010000143 DADTFG010000000
VERSION     DADTFG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.144"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            38..538
                     /gene="tssB"
                     /locus_tag="J1138_003761"
     CDS             38..538
                     /gene="tssB"
                     /locus_tag="J1138_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBA8455504.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            597..2135
                     /gene="tssC"
                     /locus_tag="J1138_003762"
     CDS             597..2135
                     /gene="tssC"
                     /locus_tag="J1138_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HBA8455505.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            2155..3492
                     /gene="tssK"
                     /locus_tag="J1138_003763"
     CDS             2155..3492
                     /gene="tssK"
                     /locus_tag="J1138_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HBA8455506.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALALGKLKTQAVRLRFADGTLIDSDVSDILPPACDLRTLEGDSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFFLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVQLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGSCMIYAPGTMGDLKPELFAVLRT"
     gene            3489..4154
                     /locus_tag="J1138_003764"
     CDS             3489..4154
                     /locus_tag="J1138_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HBA8455507.1"
                     /translation="MKKDMDINIDALLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQTIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFDVLPETAVLNVPLSTRQHPLLHSPFFWLVTLALLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4167..5819
                     /locus_tag="J1138_003765"
     CDS             4167..5819
                     /locus_tag="J1138_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBA8455508.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWSVFISPSGVLRWAGAAAIVLAVA
                     ALLIYRRRQAWTEMTGDAGLSSLPPETYRQPVVLVCGGLSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGIHRDVAVLAGRLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERASSPWFICTGGEVQVATSTETTMPAQWIAQSGVQE
                     RSQRLCYLLKAESLMQWLNLNVLTALNGPEAKCPPLAMAVGLVTSLPAVDNNLWQVWI
                     TARTGLTTDIADTGTDATLPFPDALLRQLPRQSDFTPLRRACVTMLGITTAAGITMLC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDNATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5877..6368
                     /locus_tag="J1138_003766"
     CDS             5877..6368
                     /locus_tag="J1138_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014070071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBA8455509.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6560..9205
                     /gene="tssH"
                     /locus_tag="J1138_003767"
     CDS             6560..9205
                     /gene="tssH"
                     /locus_tag="J1138_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBA8455510.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPSPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDEYPA
                     RQPQVTEPLTSALPETATADAPAKTLTEKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPEASPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSTELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDQLTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9217..11727
                     /gene="vgrG"
                     /locus_tag="J1138_003768"
     CDS             9217..11727
                     /gene="vgrG"
                     /locus_tag="J1138_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBA8455511.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGHEQLSSPFRYDIQFT
                     SSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADTQDKAQGQVREMAP
                     AMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGVAI
                     ASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAQKSIVITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIESGEFNAKFLFTDDDGLPYANTKYIACFSDGTQKE
                     GITDENGYTENFNTDSKQTIDVRLLNQNIDMILGGVHE"
     gene            11720..>12091
                     /locus_tag="J1138_003769"
     CDS             11720..>12091
                     /locus_tag="J1138_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455512.1"
                     /translation="MSNDRGSSSGENQKTEQCIQEFQVTNQFKKMMKESLEEQKKVID
                     KRVKTLTHWSDEAEDEFRRIFGVPSEKIITIKFKLNGEVTKETKSARAVIQEAVDRMK
                     FICDKLSADKGECKEVTFIDKY"
BASE COUNT         2865 a         3285 c         3504 g         2437 t
ORIGIN      
        1 ataaccatca gaaaaagtgg atgatgagga aaaggatatg gccgacagtt tccagaatga
       61 agttcccgcc gctcgtgtaa atatcaagct tgatctgcat acaggcaatg caaaaaagaa
      121 agttgaactc cccctcaagc ttcttgccgt aggcgattac agtaacggaa aagagcagcg
      181 tccgctgtcc gaacgggaca agattgatat caataaaaac aacttcaaca gcgtcatggc
      241 tgagttttcg cctgcggtta atttaacagt agaagatacg ctaagcggaa acggtaatga
      301 acaaaatatc gcgcttgagt ttaaaagcct gaaggatttt gaacccgaac aggtagcaaa
      361 aaatattccg cagcttcgtg tattactcgc tatgcgcaat ttactacgtg acctgaaatc
      421 caatctgctg gataacgcaa cgtttcgccg tgaactggaa aatatcctga aagacccgac
      481 gctcagttca gaactgcgtg atgaactggc gaaaattgcg ccgcaggaga acgtttaacc
      541 cgaacgcgta ttcaggttaa accggagaag aaaggatcac tgaaaaggaa atgctgatgt
      601 ctgtacaaca agaacatgcc acctctgaaa ctgcaacact caccaccact gagtccggcg
      661 gcgtttatca gtccctgttc gataaaatta atttaacccc ggtctcttcc attcaggaaa
      721 tagatttatg gcaaaacagc gaaacgctgg ctgatgcctc acccgatgag cgcgtgacag
      781 cagcgattca cgttctgctt tcctgtctgg cgaaatcagg cgaggacgtg gtcaagctcg
      841 acaagagcct gctggatttt catatcgacg atctggatca gaaaatcagt aaacagcttg
      901 atgcggtcat gcaccaccct gaattccaga aagtcgagtc gctgtggcgt ggtacatggt
      961 tcgtcgtaca gcgcactgat tttcgcaaaa atgtcagaat tgaattgctg gatatcagca
     1021 aagagcatct gcgtcaggat tttgatgatt caccggaaat cattcagagt ggtttatatc
     1081 gccatacata cattcaggag tacgatacgc cgggtggcga acctgtagcc tcattaatat
     1141 ccagctatga atttgataac agcccgcagg atattgccct gctgcgtaat atttccagag
     1201 tgtctgccgc ttcccatatg ccttttatcg gttctgtcgg accgaaattc ttccttaaaa
     1261 attcgatgga agaagtcgcc gcgattaaag atatcggcaa ctactttgac cgcgcagaat
     1321 atattaaatg gaagtcgttc cgcgatacgg atgacagccg ctatgtggga ttagtgatgc
     1381 cgcgcgtgct gggccgtctg ccctatgggc cggacacggt gccggtacgc agctttaact
     1441 atgtggaaga agtcaaaggc ccggatcacg aaaaatacct gtggacaaac gcctcgttcg
     1501 cctttgccgc caatatggtg aagagctttg tgaataatgg ctggtgcgtg cagatccgtg
     1561 gcccacaggc gggcggcgca gtggccgatc tgccgatcca tctttacgat ctcggcaccg
     1621 gcaatcaggt caaaattccg tccgaagtga tgatcccgga aacccgcgag tttgagttcg
     1681 ccaaccttgg ctttattcca ctctcttatt acaaaaaccg cgattacgcc tgctttttct
     1741 cggcaaactc cgcccagaaa ccggcgctgt atgataccgc tgacgccacc gccaacagcc
     1801 gtatcaacgc ccgtttgccc tatatcttcc tgctgtcccg cattgctcac tacctgaaaa
     1861 ttattcagcg cgagaatatc ggcaccacca aagaccgccg cgtgctggaa ctggagctta
     1921 acacctggat ccgcacgctg gtgacggaaa tgaccgatcc gggcgatgaa cttcaggctt
     1981 cgcatccact gcgcgacggg aaagttatcg tcgaggacat agaggacaat ccgggctttt
     2041 tccgcgtcag actctttgcc gtgccgcatt tccagatcga agggatggac gtcaaccttt
     2101 ctctggtttc ccagatgcca aaagcaaaag cctgatcggg cggtggtgtg atgtatgaaa
     2161 atttgtcgac cactgtgggc tgaggggaca ttcctgtctt ctcagcagtt tcagcagcag
     2221 gcccgctggg aagcgttcag caatgacagc attgcgcagc tttgtattcg ccatccgtgg
     2281 gggatcgcaa acgtgctctt tgaccgggat gccctggcgc tcggaaagct gaaaacgcag
     2341 gcagtacgcc tgcgttttgc tgatggtacg ctgatcgatt cagacgtctc ggatattctg
     2401 ccgcctgcct gtgacctgcg cacgctggag ggcgacagcg cgattgtttt gctggcgctg
     2461 ccgcttgcgc atggtaacgg cggtaatctc ggtcagggcg agcaggctga aaggccgctg
     2521 cgctatcgtc aggaatggca aaaggtgcag gatatctacg gcagtgacag tgaagatatg
     2581 gccgttgagc gccatgcact gtccctgcgc tttgcccatg ataacaatca ggattacatc
     2641 acctgtccgc tggcgcgtct ggtgcgggat gttcagggca actggacgca ggatgagagc
     2701 tatattccgc cgttactggc gtttaacgcc catgacgggc tggtacagcg tctggacaca
     2761 ctgctgttgc agctacgggc gaagtgccag cgtctgatgg cgatgcgccg ggaaagcaac
     2821 cagcgtatgg cggactttgc cgtcgccgat gtctccctgt tctggctgct taacgcgctg
     2881 aattccgccg aaccggtgct gagcgatttc ctgcgctacc cggcggttca ccccgaactg
     2941 gtctggcggg agctggcgag gcttgccggt gcgctgctga cgttttccct tgaacataac
     3001 gtcagcgccg ttccgcccta tgtccacgaa tctccgtcca ccgtctttcc gccacttttc
     3061 ttcctgctca gcgagctgct ggaagccagc ctgccgtcgc gggtgatcgc gctggatctg
     3121 gcgtccctgc cgggcaaccg ctggaaagcc gacctgcacg atccgcgcct gcgggaagag
     3181 gccgattttt atctgtccgt tcgttcctca ctgcctgcgc atcaggtact gcatcagcta
     3241 ccgctggtct gcaaaatcgg tgcgcccgat gatgtcacgc tgcttatcaa cgtggcgctg
     3301 aacggtgttc aactggtgcc gcttacctcg gttccggcgg cgctgccgct gcggctggag
     3361 aaccagtatt ttgcgctgga tatgcacagt gacgcggcta aatcgatgct ggagtccggg
     3421 agctgcatga tttatgcgcc gggaaccatg ggcgacctga aacctgaact gtttgcggtg
     3481 ctgcgcacat gaaaaaagat atggatatca atatcgatgc gctgctgcgc gacacgtttc
     3541 tgacggtggt ggaactgcgt cagggaacca ccgtgcgtca cggtatcgag ctgtatcgcc
     3601 actgtcagcg gcaggtggaa ctggtccgcg aacgtctgaa agatgccgga ttcagtcggg
     3661 agaacgtgga gcacataact tacgcgcagt gcgccctgct ggacgaaacg gtcctgagcc
     3721 gtagcggcat ggacgacggt cagaccatct ggatgaagga tccactccag tcgcatttct
     3781 tcaacaccct gcaggcaggt gagctgctgt acgaacggat gaagcaggtg ctacaggagc
     3841 ccgcccccgc gcaggccgtg ctgacctgct ttcaccgtgt cctgctgctg ggatttcgcg
     3901 ggcgttatca ggatccggca gccccggagc gcgaccagct tatttcgacg ctcaacgggc
     3961 aggtcgcgcc ctttgacgtg ctgccggaaa cggcggtact gaacgtgccg ttatccaccc
     4021 gtcagcaccc tttgctgcat tcgccgtttt tctggctggt aacactggcg ctgctactgg
     4081 cgggcgtctg gtgggggcta caccactggc tgaatgtact ggtagatgaa ctgctgccgc
     4141 aaagcctgcg ttaaagggag tcgtgagtga ggaacacgct gaaacaggcc atcgtgctgt
     4201 ggggaatggt gttactgctg gtgctgtggt cggtgtttat cagtccgtct ggcgtgctga
     4261 gatgggccgg tgcggcggct atcgttctgg cggttgccgc gttgttgatt taccggcgca
     4321 ggcaggcgtg gacggagatg accggcgatg ccgggttgtc atcgctgccg ccggaaacct
     4381 accggcagcc ggtagtgctg gtctgcggcg gtctgtcggc gcacctgttc actgacagcc
     4441 cggtccggca ggtttcagaa gggctgtatc tgcatgttcc tgatgaagaa cagcttgtgg
     4501 cgcaggtgga gcgattgctg acccttcgcc cggcgtgggc atcgcagctt gccgtggcgt
     4561 ataccgtcat gcccggcata caccgggatg tggcggttct ggccggacgg ctgcgacggt
     4621 ttgcccacag tatggcgacg gtgcgtcgcc gggcgggcgt aaacgtcccc tggcttctct
     4681 ggagcgggct gtccggctcg ccgttgccgg aaagagcgag ttcaccgtgg tttatctgta
     4741 ccggcggcga agttcaggta gcaacatcca cagagaccac catgcccgcg cagtggattg
     4801 cacaatccgg cgtacaggag cgcagtcagc gactctgtta cctgctgaaa gctgaaagcc
     4861 tgatgcagtg gctgaatctt aatgtgctga cggcactgaa cggcccggag gcgaaatgcc
     4921 cgccactggc gatggccgtg gggctggtca cctcgttgcc agcggtggat aacaacctgt
     4981 ggcaagtgtg gatcaccgcc agaaccggcc tgacgacgga tatcgcggac accggcacag
     5041 acgccacgct gccgtttccg gatgctctgt tacggcagtt gccgcgtcag tcggacttta
     5101 ccccgctgcg gcgggcctgc gtgaccatgc tgggcattac caccgcggcg ggtatcacca
     5161 tgttgtgcct gtcagccacg gcaaatcgcc agttattacg gcaggtcggt gacgatctgc
     5221 accggtttta tgccgtcccg gtggaggaat ttatcaccaa agcccgtcac ctgtcggtgc
     5281 tgaaagacaa tgcgaccatg ctcgatgggt attaccggga aggagaacca ctgcgcctcg
     5341 gtctggggtt ataccccggc gaacgcatcc gccagccggt attacgcgcc attcgcgact
     5401 ggcgtccgcc tgaacaaaaa atggaggtga cggcttcgct tcaggttcag accgtgcgtc
     5461 ttgacagtat gtcgctgttt gacgtcggac aggcccgcct gaaagacggc tcgacaaaag
     5521 tgctggtgga tgcgctggtg aacatccggg caaaaccggg ctggctgatc ctcgtggccg
     5581 gatataccga cgccaccggc gatgaaaaaa gcaatcagca gttatcgctg cggcgtgccg
     5641 aagcggtgcg caactggatg ctgcagacca gcgacatccc ggccacctgt tttgccgtac
     5701 agggactggg cgagagccag cctgcggcga ccaacgacac gccacagggc cgggcagtca
     5761 accggcgtgt cgaaatcagt cttgttccgc gttctgacgc ctgtcaggac gtgaaataaa
     5821 acataccgcc ggaagaaggc ggtgcttcaa tcacactaac aaggagagta attctcatgg
     5881 ctattcctgc ttatctctgg ctgaaagatg acggcggcgc ggatatcaaa ggttccgtgg
     5941 acgttcaggg gcgcgaaggt agcatcgaag tggtggcgct ggatcacgat gtgtacatcc
     6001 cgaccgacaa taacaccggc aaactgaccg gtacccgtac tcacaagccc tttacgttta
     6061 ccaaagaaat cgatgcctcc agcccgtatc tctacaaagc tgtgaccacc ggacagaccc
     6121 tgaaaacggc agaatttaaa ttttaccgca tcaacgatgc cggtcaggaa gtggagtact
     6181 tcaacatcac gcttgataac gtcaagctgg tcagagtcgc tccgcttatg cacgacatca
     6241 aggatccttc cagagagaag cataaccacc tggaacgtat tgagttccgc tacgagaaaa
     6301 tcacctggac ttacaaagac ggcaacatca ttcattccga ctcgtggaat gagcgtcctt
     6361 ccgcctgata actgcggcag acaggaaact gtctgccttt ctgttgtgtg ctttgtccag
     6421 tgccgcaggc accggtactg agcaaagcat acccacaatc tggtgaaacc tgatgggcct
     6481 gagtgaggta acggctatgg gcggtagcgt gcctgaacgc agatcgcagg aatacagaac
     6541 acgataagga aacccttcta tgacaggaaa tcaccccgcc gcgctgctgc gtcgccttaa
     6601 cccatactgt gcacgggcgc tggacgccgc cgcctcgctg tgtcagaccc gcgcccatgc
     6661 ggaaataacc attgaacact ggctgctgaa actgctggag cagggagaag gcgatatcac
     6721 ggtgattgcc cgccgctatg aatgggatat cgacacgctc tggcagtctc tgctggcaca
     6781 tctggacacc ttacccagcc cggtccgcga acgtcctcaa ctttctgaac cgctggcagc
     6841 gcttatccga caggcgtggc tgatagcgtc actggaaggc gacgatccac aaatccgcag
     6901 ccagcatctg ctgatggcgc tgacagaaaa accgatgctg cccgcctgta atgacctgtg
     6961 ggtattgctg agtctgagcc gcgtgcagct tgagcggctg cgtcccctgc tggatgcgca
     7021 gtcggatgaa tatccggcac gtcagccaca ggtcaccgaa ccgctgacct ctgcactgcc
     7081 ggagacggca acggcggacg caccggcaaa aacgctgacg gagaaacagg atgacgccct
     7141 gctggcggtg cttaaccgct ttaccgaaga cgtgacggaa aaagcccgca gcgggcgaat
     7201 cgacccggta ttcgggcgcg acacggaaat tcgccagatg gtcgatatcc tctcccgtcg
     7261 ccgcaaaaac aacccgattc tggtgggaga accgggggtg ggcaaaaccg cgctggtgga
     7321 agggctggcg ctgcgtatca ccgaaggcaa cgtgccggac agcctgaaaa cggtgcatat
     7381 ccgcacgctg gacctcggtc tgttacaggc tggcgcgggc gttaaaggtg aatttgaaca
     7441 gcggctgaaa aatgtcatcg atgcagtgca gaaatcaccg gagccggtac tgctgtttat
     7501 tgatgaagcc cataccatta tcggtgcggg taatcaggca ggcggcgcgg atgcagcgaa
     7561 cctgctgaaa ccggcactgg caaggggcga actgcgcacc atcgcggcga ccacgtggag
     7621 cgaatacaaa cagtattttg agcgcgacgc cgcgctggag cgccgcttcc agatggttaa
     7681 ggttgacgag ccggatgatg acaccgcctg tctgatgctg cggggactga aggctcgcta
     7741 tgcacagcac cacggcgtgc atatgctgga cagcgccatt cagaccgccg tgcgcctgtc
     7801 gcgccgctat ctgaccggac gccagctgcc ggacaaggcg gttgatttgc tggataccgc
     7861 cggggcaaga gtccgcatga gccttgacac cctgccggaa ccgttgacgc agcttcatgc
     7921 gcgactggcg gcactggata ttgagcggga agcgattgag caggacagcg tattttatcc
     7981 cgaagccagc ccggagcggc tggcggaact gaccgatttg cgtgatgagc tacaggcaga
     8041 agccgggcat ctggaagcgc agtatcagca ggaaaaggca ctggcgcagc agattatgac
     8101 gttgcgtcag gaaggaacag acagcactga actgcaacag caactgcgaa cgcatcaggg
     8161 ctttgcaccg ctgctggcgc tggatgtgga cgcccgcgcc gtcgctacgg tggtggcgga
     8221 ctggaccggc atcccgctct catccctgct cagggacgag cagagcgatc tgctcagtat
     8281 ggaacagagc cttgaaaacc gcgtggtcgg gcaacgcccg gcgctctgcg ccatcgcaca
     8341 gcggctgcgg gcggctaaaa ccggcctcac gccggagaac ggcccacagg gggtattcct
     8401 gctgaccggc cccagcggca ccggcaaaac cgaaactgcg ctcacactgg ccgacaccct
     8461 gtttggtggt gaaaaatccc ttatcaccat taatctttcg gaataccagg aaccgcatac
     8521 cgtttcccag ttgaaaggtt cgcctccggg ctatgtcggc tacggtcagg gcggcgtact
     8581 taccgaagcc gttcgcaaac gcccttacag cgtggtgctg ctcgacgaag tggaaaaggc
     8641 gcatcgcgac gtgatgaacc tgttctatca ggtgttcgac cggggcttta tgcgcgacgg
     8701 cgaagggcgg gaaatcgact tccgcaacac cgtcattctg atgaccgcca atctgggcag
     8761 cgaccacatc atgcagctgc tggaggaaaa accggacgcc acggacgcag acctgcatga
     8821 actgctgtat ccccttctgc gagaccattt tcagcctgca ctgatggcgc gttttcagac
     8881 ggtgatttac cgcccgctgg gacaggaagc gatgcgcacc attgtggaaa tgaaactggc
     8941 gcaggtggtc cgccgtcttc accagcacta cggactggaa acggaaatca acgacagcct
     9001 gtatgaccag cttaccgccg cctgcctgct gccggacacc ggtgcgcgta atatcgacag
     9061 cctgctgaac cagcaaatcc tgccggtctt aagccagcag ttgctggcac agcaggccgc
     9121 gcaccgtaaa ccagcccaac tacggcttgg ctgggatgag gaggacggga ttgtactgga
     9181 gttcgctacg gaagagatgc aataacggag gctgtcatgg gtattcagaa tttcgaccac
     9241 tcacaccaca agctcaaaat tcgcggcctg aaaagcccgg tggacgtgct gacttttaca
     9301 ggccacgaac agctcagctc acccttccgc tacgacattc agtttaccag cagcgataaa
     9361 gccatcgcgc cggaatcggt gctgatgcag gacggcgcat tcagcctgac ggcaccgccc
     9421 gtacagggaa tgccggtaca gactgccctg cgcaccctgc acggcgtcat taccggcttt
     9481 aaacatctct catcgtcaca ggacgaagcc cgttacgaag tgcgccttga accccgtatg
     9541 gcgctgctta cccgcagccg ccagaacgcc atctaccaga accagacggt gccgcaaatc
     9601 gtcgaaaaaa tcctgcgcga gcgacaccag atgcgcggtc aggacttcgt gtttaacctc
     9661 aaaagcgaat acccggcccg cgaacaggtg atgcagtatg gcgaggacga cctgacgttt
     9721 gtctcacgcc tgctgtcgga agtgggtatc tggttccgct tcgccaccga cgcccggctg
     9781 aaaatcgaag ttatcgaatt ttacgacgac cagagcggct atgagcgcgg gctgacgctg
     9841 ccgcttcgcc atccttctgg gctgtttgat ggtgaaacgg aagccgtatg ggggctgaac
     9901 accgcgtaca gcgtggtgga aaaaagcgtc accacgcggg actacaacta ccgcaccgcc
     9961 acggcggaga tgatgacaga gcagcatgac gccaccgggg gcgataacac cacctacggc
    10021 gaagcctatc actacgccga caacttcctg caaaaaggcg ataaagaggc agcggaaagc
    10081 ggcgcgtttt acgcccgtat ccgccacgaa cgctacctga atgaacaggc tattctcaaa
    10141 ggtcagagca ccagttcact gctgatgccg gggctggaga taaaagttca gggcgacgac
    10201 gcgccagcgg tgttccgcaa gggcgtactg attaccggcg tgacggccag cgcggcgcgt
    10261 gaccgcagct atgaactgac gtttaccgct atcccgtact cagaacgcta cggctaccgc
    10321 ccggcgttta tcccccgccc ggtcatggcc ggaacgctcc cggcgcgggt caccagcacg
    10381 gtgaagaacg atatctatgc ccatatcgac aaggacgggc gctaccgggt caaccttgac
    10441 tttgaccgcg atacgtggaa accgggctat gagagcctgt gggtacgcca gtcgaggccc
    10501 tatgccgggg atacctacgg cctgcacctg ccgctgctgg caggaacgga agtcagcatc
    10561 gcctttgagg aaggcaaccc ggacaggccg tatatcgccg gggtaaagca cgactcggcg
    10621 cacaccgacc atgtgaccat ccagaactac aagcgcaacg tgctgcgtac cccggccaac
    10681 aataaaatcc gcctcgacga tgagcgcggc aaagagcata tcaaggtcag caccgaatac
    10741 ggcggcaaga gccagctaaa cctcggtcat ctggtggatg ccgggaaaca gcagcgcggg
    10801 gaaggctttg aactgcgtac tgatctgtgg ggtgcggtgc gggcaaagaa aggcatcttt
    10861 atcagcgccg atacgcagga caaggcgcag ggtcaggtgc gcgaaatggc tccggcgatg
    10921 gcaatactgg atggcgcaca gtcacagatg aagtcgctgt ccactgatgc gcagacggcg
    10981 aatgccgatc cggcagatct gtcatcacag atagcgctgt tgcagcagag tgtaaaagat
    11041 ctgacgcagg ctgcgatcct gctaagcgca cccaaaggcg tggcgatcgc cagcggagag
    11101 cacctgcaac tggcggcatc gaaaaacctg atcgcgaacg caggaaatca cgctgatatc
    11161 ggtgttgtga agaatatgtt tatcggtgtg ggtcaggcgc tgagcgtgtt tgtgcgtaaa
    11221 gcgggcatca aactgtttgc caacaagggg gcgatctcgg tacaggcaca gaacgacctg
    11281 atggaactgt tggcacagaa atcgatagtg atcaccagta cggaagatga gatcaagatc
    11341 accgcgaaga agaaaatcac ccttaacggc ggtggcagct acatccggct ggatgcctgc
    11401 ggaatagaag cgggaacgcc gggagaatat aacgttaagg cgggatatta tgggcgtaag
    11461 ccgaaggcga agctgacacc ggagttgatg gcgtttccag tgattgagag cggggaattt
    11521 aatgctaagt tcctttttac tgatgatgat ggtctacctt atgccaacac aaaatatatt
    11581 gcatgttttt ctgatggcac tcaaaaagaa ggtattactg atgagaatgg gtatacagaa
    11641 aattttaata ctgattccaa acaaacaatt gatgtcagat tattgaatca aaatatagac
    11701 atgatattgg gaggcgtgca tgagtaatga tagaggtagt tcaagtggtg aaaatcagaa
    11761 aactgaacaa tgcatacagg aatttcaagt tacgaatcaa ttcaagaaaa tgatgaagga
    11821 gtctctagag gaacaaaaaa aggtaattga taaaagggta aaaacattaa cgcactggag
    11881 tgatgaagca gaagatgaat tcaggagaat atttggcgtg ccatctgaaa aaataataac
    11941 cataaaattt aaactgaacg gagaagtaac aaaagaaaca aaaagtgcgc gagcagttat
    12001 tcaagaagcc gtggacagaa tgaaatttat atgtgataaa ttatctgctg ataaagggga
    12061 gtgcaaagaa gttactttta tagataaata t
//
LOCUS       DADTFG010000144        11872 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.145, whole genome shotgun sequence.
ACCESSION   DADTFG010000144 DADTFG010000000
VERSION     DADTFG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.145"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            10..612
                     /locus_tag="J1138_003770"
     CDS             10..612
                     /locus_tag="J1138_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBA8455513.1"
                     /translation="MNINYPAEYEIGDIVFTCISAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(614..1135)
                     /gene="ruvC"
                     /locus_tag="J1138_003771"
     CDS             complement(614..1135)
                     /gene="ruvC"
                     /locus_tag="J1138_003771"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBA8455514.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1170..1910)
                     /locus_tag="J1138_003772"
     CDS             complement(1170..1910)
                     /locus_tag="J1138_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA8455515.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1939..2391)
                     /gene="nudB"
                     /locus_tag="J1138_003773"
     CDS             complement(1939..2391)
                     /gene="nudB"
                     /locus_tag="J1138_003773"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBA8455516.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2384..4156)
                     /gene="aspS"
                     /locus_tag="J1138_003774"
     CDS             complement(2384..4156)
                     /gene="aspS"
                     /locus_tag="J1138_003774"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBA8455517.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4466..5032
                     /locus_tag="J1138_003775"
     CDS             4466..5032
                     /locus_tag="J1138_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA8455518.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPYTADEVVNRAGKLAAKF
                     RANGQPVFLVRVGWSADYAEALKQSVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5029..5847
                     /locus_tag="J1138_003776"
     CDS             5029..5847
                     /locus_tag="J1138_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBA8455519.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIVQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5900..6295
                     /locus_tag="J1138_003777"
     CDS             5900..6295
                     /locus_tag="J1138_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455520.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6336..7079
                     /gene="cmoA"
                     /locus_tag="J1138_003778"
     CDS             6336..7079
                     /gene="cmoA"
                     /locus_tag="J1138_003778"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBA8455521.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7076..8047
                     /gene="cmoB"
                     /locus_tag="J1138_003779"
     CDS             7076..8047
                     /gene="cmoB"
                     /locus_tag="J1138_003779"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBA8455522.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVMPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8212..10641)
                     /locus_tag="J1138_003780"
     CDS             complement(8212..10641)
                     /locus_tag="J1138_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HBA8455523.1"
                     /translation="MTLTRREFIKHSGIAAGTLVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPV
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGITAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAVQA"
     gene            complement(10666..11766)
                     /locus_tag="J1138_003781"
     CDS             complement(10666..11766)
                     /locus_tag="J1138_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HBA8455524.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVTPGTD
                     LTVVDASGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALIDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNTWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2716 a         3147 c         2907 g         3102 t
ORIGIN      
        1 aatgacaacg tgaatattaa ttatcctgct gaatatgaaa ttggtgatat cgtctttaca
       61 tgtataagtg ctgccttatt tggtcaaata tcagctgcat caaattgctg gagtaatcac
      121 gtcgggatca ttatcggtca taacggtgaa gactttctgg ttgcagaaag ccgtgttccc
      181 ctctcaacca tcactacgct atcccgtttt attaaacgct ctgctaatca acgctatgct
      241 ataaagcgat tagacgccgg actaacagaa caacaaaaac aacgaattgt tgaacaggtt
      301 ccttcccggc tacgcaaact ttaccacacc ggttttaaat acgaatcttc gcgccagttc
      361 tgttcaaaat ttgtttttga tatttataaa gaggcgctat gtattccggt gggtgaaata
      421 gagacgtttg gagaattgtt aaatagcaat ccaaatgcaa aactcacttt ctggaaattc
      481 tggttcttag gttctattcc gtgggagcgt aaaaccgtca cgccagccag tttgtggcat
      541 catccgggtt tggtgttgat tcacgcggtg ggagttgaaa cgcctcagcc tgaactgacc
      601 gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagtcgcgat tcgctcattt
      661 gcatcgcatt ctgactaacg tggcagtggg tgatggcaat cgccagcgca tcggcggcat
      721 ccgcctgtgg attagcgggc agtttcagca aggtgcggac catgtgctgc acctggcttt
      781 tttcggcact accaatacct accactgttt gctttacctg acgtgccgca tattcaaata
      841 ccggcaattc ctgattcacc gccgccacaa tcgccacgcc gcgcgcctgc cccagtttca
      901 gggctgagtc agcgttcttc gccataaaga cctgttcaat ggcgaaataa tcaggctgga
      961 attgggtgat gatttccgtc acgcccgcat agatgagctt cagacgagac ggtaaatcat
     1021 ccactttggt gcgtatgcat ccgctaccca ggtaggacag ttgcctgcct acctggcgga
     1081 tgacgccata gccggtcacg cgcgaacccg ggtcaatgcc gagaataata gccatcacgc
     1141 gtctccgttt tgctgtttag caggcctcat cagagagtcg ctgcaacctc atcagagatt
     1201 tcaccgttat ggtaaacttc ctgcacgtcg tcgcaatctt ccagcatatc gatcagacgc
     1261 atcagtttcg gtgcggtttc tgcatccata tcagctttgg tggacgggat catggaaact
     1321 tccgcgctgt ctgctttcag acctgccgct tccagagcgt cgcgtacttt gcccatttct
     1381 tcccatgcag tgtagacatc aatcgcgccg tcatcatagg tcacaacgtc ttcagcaccg
     1441 gcttccaggg ctgcttccat gatggtgtct tcatcgcctt tctcgaagga gatcacgcct
     1501 tttttgctga acaaataagc tacggaacca tcagtaccga ggttaccgcc acatttgctg
     1561 aatgcatgac gcacttcagc aacggtacgg ttgcggttgt cagacagaca ttcaatcatg
     1621 attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt tgcatcatca
     1681 tcaccgccca cacctcgtgc aattgcgcgg ttcagagtgt cacgggtcat gttgttagac
     1741 agtgctttat caattgctgc acgcaaacgc gggttagcgt ccggatcacc accgcccagc
     1801 ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg cttagcatcc
     1861 tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat aaaaatatct
     1921 ccagatagcc ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg cctgccggtt
     1981 gctccaggac ttagtgagcg ccgccgcagc agacgcatca agccacttgt aagccagatg
     2041 ttcagtgaaa acgatctggc gctcgtgcgg aagcgcaaga cagaaccatg attccgtatt
     2101 acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa actctaccgt
     2161 gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga cctcttcctt
     2221 tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc cggttaccga
     2281 ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg tatcttgtgc
     2341 gtagatgacc actaagatcg aaacgggacg cttataagcc atatcagtta ttctcagcct
     2401 tcttcacaac ctgaatgctc agctcagcca gtgcagtcgg gttagcaaag ctcggtgctt
     2461 cagtcatcag acacgctgcc gccgtggttt tcgggaaggc gataacgtca cggatattgt
     2521 cggtgccggt cagcagcatc gtcagacggt caagaccgaa tgccagacct gcgtgcggcg
     2581 gagtaccgta tttcagggcg tcgagcagga agccgaattt ctcgcgctgt tcctcttcgt
     2641 tgatacccag aataccaaac accgtctgct gcatatcacc attatggata cgcacagaac
     2701 caccgcccac ttcgtaacca ttgatgacca tatcgtaagc gttagccacc gcattttccg
     2761 gtgcagcttt cagttctgct gccgtcatgt ctttcggtga ggtgaacgga tggtgcattg
     2821 ctgtcaggcc gccttcaccg tcgtcttcaa acatcgggaa gtcgataacc cacagcggtg
     2881 cccatttgct ttcgtcggtc agaccaaggt ctttacccac tttcaggcgc agtgcgccca
     2941 tcgcgtcggc aacaattttc ttgttgtcgg caccgaagaa aatcatatcg ccatcttgcg
     3001 cgccagtacg ctccaggatg gcttcgatga tttctgcatt aaggaacttc gctaccgggc
     3061 tattgatacc ttccagacct ttcgcgcgtt cgttaacttt gatgtaagcc agacctttcg
     3121 cgccgtagat tttaacgaag ttaccgtatt cgtcgatctg cttacgggtc aacgatgcgc
     3181 cgcccggaac gcgcagagcg gcaacacggc ctttcggatc attcgccgga cctgcaaata
     3241 ctgcaaactc aacagatttc agcagatcgg caacgtcggt cagttccatc gggttacgca
     3301 gatccggttt atcagaacca taacggcgtt ctgcttctgc aaaggtcatt accgggaaat
     3361 cgcccagatc cacgcccttc acttccagcc acagatgacg caccagcgct tccatcactt
     3421 cacgcacttg cggcgcggtc atgaaagaag tttccacatc gatctgagta aattcaggct
     3481 gacggtcagc acgcaggtct tcgtcacgga agcatttaac gatctgatag tagcggtcaa
     3541 atccggacat catcagtagc tgtttgaaca actgcgggga ttgcggcagc gcgtagaatt
     3601 tacctttgtg cacacgagaa ggcaccaggt agtcacgcgc gccttcaggc gtggctttgg
     3661 tcagcatcgg agtttcgatg tcgaggaagc cgtggtcatc cataaaacgg cgcaccaggc
     3721 tggtgatttt agcgcgggtt ttcaggcgct gagccatttc cgggcgacgc aggtcgaggt
     3781 agcggtattt cagacgcgct tcttcggtgt tgacgtggtt agagtcaagc ggcagaacat
     3841 ctgcacggtt gatgatagtc agcgaggacg ccagtacttc gatttcgcca gtcgccatat
     3901 cgcggttaat atttttttcg tcacgcgcac gtacggtgcc cgtgacctga atgcagaact
     3961 cattacgcag ttcagaggcc agctttaacg cgtccgcacg atccggatcg aaaaatacct
     4021 gcacgatacc ttcgcggtcg cgcatatcga tgaagatcag gctaccaaga tcacgacgac
     4081 ggttgaccca accacacaga gtcacctgct gccccacgtg ggacaaacgg agctgtccac
     4141 aatattctgt acgcatgaga tatcccttaa cttagctgcc ggcggatgcc ccctgctgcg
     4201 caggtgacca agtcgcagcg ttagctgtat gtcacaactg aatgaaaaaa ggcggctatt
     4261 atactggaaa ttctgccgca ccgtaagagc ctggcccgcg ctggaacgcc tcgttaccac
     4321 tttatatcgg gcctgaaatc agactctacg ccagtttgct ataaaggtgt tgcccgaact
     4381 cataaaaatt aacaaaattt gtcgttccgc catcggctaa tcgcattaag gtgagaggca
     4441 cgattttgtt ttatcaggag tcatcatgct tgaacttaat gctaaaacca ccgcgctggt
     4501 ggtgattgat ttacaagaag gcatcttacc ttttgccgga ggtccatata ctgccgatga
     4561 ggtggttaat cgcgccggga agctggcggc gaaatttcgc gcgaacggtc agcccgtgtt
     4621 tctggtgcgc gttggctggt ctgccgatta cgccgaagca ttaaaacagt cggttgatgc
     4681 cccctccccc gcaaaagtgt tgcccgaaaa ctggtggcaa catcctgctg cattaggtgc
     4741 aaccgacagc gatatcgaaa tcatcaaacg tcaatggggt gcgttttacg gtacggatct
     4801 ggagttgcaa ttacgccgcc ggggtatcga tacaatagtg ttatgtggga tctcgaccaa
     4861 tatcggtgtt gaatccaccg cccgcaatgc ctgggaactc ggttttaatc tggtgattgc
     4921 cgaagatgcc tgtagcgccg ctagcgccga gcagcacaat aacagcatta atcatatcta
     4981 cccgcgcatc gcccgtgtgc gtagcgttga agagatcctc aacgcgttat gatttacatc
     5041 ggtttgccac aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag
     5101 tatgcccgcc actttaactg tgtggagggt aacaccacgc tttatgccct gccgaaaccc
     5161 gaggttgtcc tgcgctggcg tgagcagacc acagatgact tccgcttctg ttttaagttt
     5221 ccggcgacta tttcgcatca ggcagcatta cggcattgcg atgatttagt gactgaattt
     5281 ttgacccgca tgtcaccgtt ggctccgcgc attggacaat actggctgca actgcctgcc
     5341 acattcggcc cacgggagct gcctgcgctt tggcattttc tcgattctct tcccggcgaa
     5401 tttaattatg gtgtggaagt ccgccatcca cagtttttcg ccaaagggga ggaggaacaa
     5461 acgcttaatc gcggtttaca tcagcgcggc gttaatcggg tgattttaga cagccgcccg
     5521 gttcatgcag cacgtccaca cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa
     5581 gtgccggtac atgctgtact gacggcgaaa aatccactga tccgttttat cggtagtgat
     5641 gatatgacgc aaaaccggga attatttcag gtctggttac aaaaattagc gcagtggcat
     5701 cagaccacta cgccttatct ttttttacat acgccagata ttgtccaggc cccggaactg
     5761 gtacataccc tgtgggaaga cttacgtaaa acgcttccag agatcggagc agttccggct
     5821 attccacagc aatcttctct tttctgaatt tgccacctat catagacagg tgccatcggc
     5881 cattttaaag ggagtttgta tggtaagcgc gctgtatgcc gttttaagtg cgttgttatt
     5941 aatgaagttc tcttttgatg tcgttcgcct gcgaatgcag taccgcgttg cctatggcga
     6001 cggcggtttt agcgaactgc aaagcgctat tcgcattcat ggtaacgcgg tggaatatat
     6061 tcctatcgcg attgtgttga tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt
     6121 gcatatttgc ggcatcgttt tgcttgctgg tcgtctgatg cattattacg gttttcatca
     6181 ccgtctgttc cgctggcgac gttctggcat gagcgccacc tggtgtgcgc tgttgctgat
     6241 ggtgctggcg aatctttggt atatgccctg ggagttggtt ttctccctgc gttagcgcac
     6301 aatacgccac tttctttttc ccggattttt acgttatgtc tcaccgcgac acgctatttt
     6361 ctgcccctat cgccagactg ggcgactgga cctttgatga acgggtagct gaagtcttcc
     6421 cggatatgat ccagcgttcc gttcccggct attccaatat tatttccatg attggtatgt
     6481 tagccgagcg cttcgttcaa cctggtacgc aggtttacga tctggggtgt tctctgggcg
     6541 cggcgacgct ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt attgccatcg
     6601 ataactcccc ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat aaagctccta
     6661 cgccagtaga cgttattgaa ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg
     6721 tggtgctgaa ttttaccctg caattcctgg aaccttccga gcgccaggcg ttactggata
     6781 aaatttatca agggctgaac ccgggcggtg cgctggtgct ttcggaaaaa ttcagtttcg
     6841 aagatgccaa agttggtgaa ttgctgttca acatgcacca cgactttaaa cgtgccaacg
     6901 gttacagcga actggagatc agccagaaac gcagcatgct ggaaaacgtg atgctgaccg
     6961 attccgtgga aacccataaa gcacgcctgc ataaagccgg ttttgagcat agcgagctgt
     7021 ggttccagtg ctttaacttt ggttcactgg tggcattaaa agcagaggac gctgcatgat
     7081 cgactttggt aacttttatt ctctgattgc caaaaatcat ctttcacact ggctcgaaac
     7141 gctgcccgcg cagattgcta actggcagcg cgagcagcag cacgggctgt ttaagcagtg
     7201 gtccaacgcg gtggaatttc tgcctgaaat taaaccgtat cgtctggatt tattgcatag
     7261 cgtaaccgcc gaaagcgaag agccactgag cgccgggcaa attaagcgca ttgaaacgct
     7321 gatgcgcaac ctgatgccgt ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga
     7381 taccgaatgg cgttccgact ggaaatggga tcgcgttatg ccccatcttt ctgatttaac
     7441 cgggcgcacc attcttgatg ttggctgcgg gagcggttat cacatgtggc gcatgattgg
     7501 cgcaggcgcg cacctcgcgg tggggatcga ccctacgcaa ctgtttctgt gccagtttga
     7561 agcggtgcgt aaactactgg gtaacgatca gcgcgcacat ttgttaccgt taggtattga
     7621 acaacttccg gcactgaaag cctttgatac cgtcttttcg atgggcgtgc tttatcatcg
     7681 tcgttcaccg ctggagcatc tctggcagtt aaaagaccaa ctggtgaatg aaggcgaact
     7741 ggtgctggaa acgctggtta ttgatggcga cgaaaacacg gtgctggtgc cgggcgatcg
     7801 ttacgctcaa atgcgtaatg tctatttcat tccttccgcg ctggcgctga aaaactggct
     7861 gaagaagtgt ggttttgttg atattcgcat tgcagatgtg agcgttacca ccacagaaga
     7921 gcagcgacgc accgaatgga tggtcaccga gtctctggcc gattttctcg acccgcatga
     7981 tccgggtaaa acggtggaag gttatcctgc gcctaaacgc gcggtgctga ttgcgcgcaa
     8041 gccgtaaagg tctggtaata ctgccggatg cggcgtgagc gccttatccg gcctacaaaa
     8101 ccttgctaat tcaatatatt gcagggacta tgtaggcctg ataagcatag cgcatcaggc
     8161 agttttacgt ttgcataacc tcagcgcccg tttccgggcg cttttcacgt cttatgcctg
     8221 aacagctggc tgatcaaacg ccgttaactt cagcgcattg cccgtatatt tttcgatata
     8281 caccagcgca gagttaccgg cacaggcatt cgccagctgc gagctgggga tatccgccgt
     8341 taacacgttc gcactaccgt ttttacacaa gccattttcc agatctggcc atgcaccttc
     8401 atgcacgcat accacgcctt ttttgatccc gtcagtcacc accgcgcctg tcagaatctg
     8461 accgcgcttg ttccagacac gcaccagatc gccattcgca ataccaaagc gagcagcatc
     8521 ttcggtgtga atagtgattg gttcacgatc ggcaaccgca tattttttac gcagttccgc
     8581 atagttaagc tgactgtgta aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt
     8641 ctcgtcggcg gtacccttcc actcatcagg cgcaagccag gttgggtgtg ccgggcaatc
     8701 cttatagcca aatttttcca gcgttctgga gtaaatctca attttgccgc ttggcgtacc
     8761 cagcgcattt ttcaccggat cggcgcggaa atcaccataa cgaatgtact gttcgttctt
     8821 ctcgctgcgg cgcatttcga tcagtttatt ttgctgccag aaggcattaa acatcggcat
     8881 agtcacgcgt tgcgcacggg cacctttctg agcggcatca tagaaaaatt tcagccacgc
     8941 catttcatct ttaccttcgg tatagatctc ttttccgcca ggtttgagta attcagcaag
     9001 atcggcaaac acgtcaaaat cgttgcgcgc ttcaaattgc ggagcgacag cctgcttcat
     9061 cggcacaata tgctggttgc tgtaatcacc ggtcatcgtc aggtcattgc gctcaaacga
     9121 tgtggtgatc ggtaatacga tatctgcgtg tttggctgct gcggtccagt agcattcaga
     9181 aacgacgatc atctccggtt tctgccatgc tttaatcaga cgattagtat cctggtgatg
     9241 ggtaaagtta ccgccacccg cccaccagat cattttgata ttcggatacg tttgttcttt
     9301 accgttatgc tgatatttgc cgccaggatt ttccagcgca tcgacgatac gggcaacagg
     9361 aatcgccgta attccgccat catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga
     9421 catttctggt agcacgccgc caacacgcgt cgggttgcca ccgttggagt aatgataaga
     9481 gaaaccaaac ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag
     9541 catccagtgt ttctgttcac catattgctg gcgctgaatt ccccagcctg ccatcagcat
     9601 tgtgcgattt gccgccatca gttcagcgag tttgactatc tgcgcttccg gtacgccagt
     9661 aatttccgcc gcccaggccg cgctcttcgg cgtattatcg ctcttacctg tcagatactc
     9721 ttcaaactgc ggatacccgg tagtgtattt ttcaaggaac actttatcgt gtttgccttg
     9781 tgtcatcagg gtatgcgcaa tccctaacat cagtgccaca tcggtgccca tattcggtgc
     9841 gatccaggtg gcattatcgc caaagaattc gatggtttcg gagcggatag gatcaatggc
     9901 aatcactggt ttgccagatt ttttcagctg atggaagtat tccagccctt gctcatcggt
     9961 actgctccag gcaattttga gcgtgttcag cgggttcatc ccccacagca ccaccacctg
    10021 gctgttttcc agaataagcg gccaggaggt ctgctgttca tacacctcta cagaaccgac
    10081 cacatgcggc atgatcacct gtgccgctcc ggttgaataa tcgccgctat gcccggaata
    10141 accgcccgcc aggttcatat aacgttgcag taaggtttgc gctttatgca acacaccaga
    10201 agaacgccag ccataagatc cggcaaaaat ggccgatggt ccgttagctt tgcgaatgcg
    10261 atcgtgttgt tcatgaatca gttttaatgc ttgttcccag ctcacctgta cataggtatc
    10321 ttcgccacga cctttcaccg gttgcagtgg attatcgaga tagctttttc tcaccatcgg
    10381 atgctgaata cgcgccgtgg tgtgtacctg atccgccgcc gtagactgta aggagttcgg
    10441 tatggttttc gccagcgcgc ctgtcgaaga aacaatcttg ccgtccttca cttctacgtt
    10501 catcgctccc caacggcccg cagtgagaat tttgccgccc ttctcttctg cccatgcggg
    10561 tagcggtgca gccgatgtca ccaccagcgt ccctgccgca ataccgctat gtttaataaa
    10621 ttcacgtctt gttaatgtca taacttcctc cctgatcaac gaggatcact gtttctcggt
    10681 aatatctttg gcgttgtact ggaagtaccg cgtcaaaatg tccagttcgt tttcgctcat
    10741 gctggttcgc gcacccattc ctttggcaat ggacggccat gtattgacgg tgtaatggtc
    10801 ggcggcaata ggggcatggc agccagcgca ataggtatcg tcaagttttt cagcgtattg
    10861 ccatagcggt ttacggtccg ctaatgcggg atcgataagc gccccctgca aagacgcctg
    10921 acgccattga ttgccgtatt cgtcagcctg ccattccccg tttaccgtta gcgccttgat
    10981 accttcttca cttaatgtgg cgagcgccag ccgttggcct gccgccaggt agagcgtgtt
    11041 ttcactgccc tgcatttgat aaccctgcaa ccgaacaatt ggctgtttgc cactggcatc
    11101 aacgacggta agatcggtac caggagtcac ggtagccagt tcgcctatgc gagaagtttt
    11161 gaaaggataa atatgtgcgc cattagtaac tgaagtagcg gcctgacttt ccagctcatg
    11221 cgccgcgttg tcatccattt ttatttctgg cggaaaatgg gcaatgcctt tatggcaatc
    11281 gatacaggtt tcgccgtcct tttgcgcttt attatgcatt ttctgcgcag attcactttg
    11341 cgaggcaata tccatggcat caaaagaatg gcaactacgg caggttgcag agtcagttgc
    11401 ttttaattct ttccatactg tttcggccat ttcctggcga tgagcttcga acttatcgtc
    11461 actgtctatt ttgccgctaa caaattcatg ataaatatct ttagatgctt ttaatttagc
    11521 aaataaataa tccatccctg actttggaat atggcaatca gcacattccg cacgtatccc
    11581 tttctggttt gaaaagtgga cagttccctg atattcctca aaaggtttac tcatcgagtg
    11641 gcaggaaagg caaaatgcgg tatccgacgt tttatgtaag gctttttgcg ccagcaataa
    11701 cccgccgcca ccaaccacaa ccgctatcag caaaaacaat aacccaatgc gttttttccc
    11761 tcgcatagat tcctctaatt aaattccagg acaccgatac cattgtataa ataaatacac
    11821 aacgaattga ctacttgctt aattcggcac aacactgcct gaaataatcg at
//
LOCUS       DADTFG010000145        11736 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.146, whole genome shotgun sequence.
ACCESSION   DADTFG010000145 DADTFG010000000
VERSION     DADTFG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.146"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(13..294)
                     /locus_tag="J1138_003782"
     CDS             complement(13..294)
                     /locus_tag="J1138_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBA8455525.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(557..1666)
                     /gene="apbC"
                     /locus_tag="J1138_003783"
     CDS             complement(557..1666)
                     /gene="apbC"
                     /locus_tag="J1138_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBA8455526.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1798..3831
                     /gene="metG"
                     /locus_tag="J1138_003784"
     CDS             1798..3831
                     /gene="metG"
                     /locus_tag="J1138_003784"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBA8455527.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3972..7760
                     /locus_tag="J1138_003785"
     CDS             3972..7760
                     /locus_tag="J1138_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBA8455528.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTQGQSQVKSFAD
                     AVAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNTTATFDFSACSLEWQNTVAQAISQIDDLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAVPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     VASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPGEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIT
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWTGRKCPSGRVMGLANKGWIKGTPQDAGWIGWMINPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7770..8012
                     /locus_tag="J1138_003786"
     CDS             7770..8012
                     /locus_tag="J1138_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBA8455529.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKTLSGVMKTFHWSGSPPSRRPST"
     gene            8048..11401
                     /locus_tag="J1138_003787"
     CDS             8048..11401
                     /locus_tag="J1138_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBA8455530.1"
                     /translation="MAALACIAQNDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYES
                     DPLVVTLQYQNEDYGYGYRSETYNEFDLRLRKHLSLAEETCWQRCADKLIAALPGITK
                     VRRPFIALNLPEKPEIANELVSLECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIF
                     SDREASYMSHENHFGYAACAALLREQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRT
                     LLLVADKNKPSLQRVAKYSKNFPHATLAALAELLALKAPPARPGYPIIEDKKLPAQQK
                     ARDEYWHTLLQTLMASQPQLAEEVMPCLSTQAQAVVNGYLSASPKLAFESTHSNDLPE
                     ILVSPPWRGKKKTALLRLDLVPLELAPKARWQPGERERLAATESARYFSTGSFTERME
                     RKSGRVVLQELGFGDDVWLFRNYILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTE
                     AKSAEQALRSGDVEALINTWENDSYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEK
                     KHLFAGEDYFLSILGLDTLPGLLLAFSHHPKETFPLILNFGATELALPVARVWRRFAA
                     QRDLARQWILQWPEHTATALIPLVFTKPSDNSEAALLALRLLYEQGHGELLQTVANRW
                     QRTDVWPALEQLLKLGPIEIYPARIPKAPDFWHPAMWSRPRLIKNNQPVTDDALEIIG
                     EMLRFTQGGRFYGGLEQLKTFCQPQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFG
                     DESTARDLTTQILAWPQEGKSARAVSGLNILTKMNNDMALIQLHYISQRAKSRPLRDN
                     AAEFLQVVAENRGLSQEELADRLVPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFD
                     QQNVRQKSVPRLRADDDQLKTPEALARLKGLKKDATQVSKNLLPRLETALRTTRRWSL
                     ADFHSLFVNHPFTRLVTQRLIWGVYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPAD
                     ALIGIAHPLEMTAEMRSEFAQLFADYEIMPPFRQLTRRTVLLTPDESASNSLNRWEGK
                     SATVGQLMGMRYKGWESGYEDAFVYDLGAYRLVLKFSPGFNHYSTDSKALMSFRSLRV
                     YRDNKSVTFAELDVFDLSEALSAPDVIFH"
     gene            11462..11736
                     /locus_tag="J1138_003788"
                     /pseudo
     CDS             11462..11736
                     /locus_tag="J1138_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3048 a         2995 c         2895 g         2798 t
ORIGIN      
        1 cagggtggca aattaatcgt agcgatttaa cacgattcga cgtttgctat cgctatctgc
       61 agcaatcact tccgtcgtta cacctttaac tggcgaagaa acctgatgac gggtatcaac
      121 attatacgtt aactgaccac aatgctgttg aggttgacca ttaaccatag ccatactgca
      181 tgttggggga ctgatcctac cattaatcgt taatgtagca gtttctgaag agaacgcggg
      241 agaagccaat ccataggcaa gaaaaataat tcctgacagc cagtacttat tcattgaacg
      301 ttatccctgt agtaaaggtt atgcctggaa agaatgagtg cataacaaac tatagctgta
      361 catccactac acagccacga aggatgataa tgaagcatcg ccggtacgat caatcgactt
      421 tgtagagttt cggacgaagg tccgcagaat attcgcagca ttaaataagt gttcattaat
      481 gcggcaaaaa caaatacacc gcatcaatgt aacatctctg cggtgtatga agaataacca
      541 gaatggttaa tacgcgttag accgcgcgga aggaaatctc gcctggaatg acttcaccct
      601 gccagtagag ctgagctgca acgcggtcag ccagctggcg gtagatagcg gtaaattcgc
      661 tctcaggacg gctgataacc gttggcgtgc ctttatcgag atcctcacgt aaggagatgt
      721 gtaacggcat ctgccccagc agctgtgtgt ggtatttctc ggccagtttc tctgcaccac
      781 cggtaccgaa aatcggttca tggtgaccac agttactgca aatatgcaca ctcatgttct
      841 cgacaatacc gagcaccgga acctcgactt tctcgaacat cacaatgcct tttttcgcat
      901 cgatcaacgc gatgtcctgc ggggtggtga ccacgactgc accggttact ggaatgtttt
      961 gtgccaatgt caattgaatg tcaccggtac ctggcggcat atcaagcacc agatagtcga
     1021 gatccggcca caaggtttcc tgcaacattt gcattaacgc cttgctcgcc attgggccac
     1081 gccacaccat cgcattgtcg tctgtcacca gataaccaat tgaattagtt gccaggccat
     1141 gagacataat cggtgccata tgggtaccgt ccggcgaagt tggacgctga ttttctgcgc
     1201 ccagcatggt agggattgat gggccgtaga tatcggcgtc cagaatgccg actttcgccc
     1261 cttccgccgc cagtgccaac gccaggttta ccgccgtgga ggatttaccc acgccacctt
     1321 taccggaact gaccgcgata atgttcttca cgccgttaat tcccggctga ttcttcacgc
     1381 gtttcagagt ggctatgtta tgcgacagtt tccagtcgat agctttcgct ccggtgatgc
     1441 gcagcagttc ggcgctacat tgctctttta actcttcgaa cgcgctatgc cagacgaatg
     1501 gcataaccag ttccacatgc agcgtgtcgt ccatccaggc gacatggtgc aacgctttca
     1561 gcgttgtgag gttatgcttc agggttgggt gctgaaaatt ggccagtgtc ccggcgacca
     1621 ttgcgcgcaa ggcttctggt gatttggcct gggattgttc gttcatcccg actccttttt
     1681 tgtatagata aaccatcagc tgatagttta cctgaagaat atagagaagt acttacttaa
     1741 cattttccca tttggtacta tctaagccct tttcactatt aagaagtaat gcctactatg
     1801 actcaagtcg cgaagaaaat tctggtgacg tgcgcactgc cgtacgctaa cggctcaatc
     1861 cacctcggcc atatgctgga gcacatccag gctgatgtct gggtccgtta ccagcgaatg
     1921 cgcggccacg aggtcaactt catctgcgcc gacgatgccc acggcacgcc gatcatgctt
     1981 aaagctcagc agcttggtat caccccggag cagatgattg gcgaaatgag tcaggaacat
     2041 cagactgatt tcgcaggctt taacatcagc tatgacaact atcactcgac gcacagcgaa
     2101 gagaatcgcc agttgtcaga acttatctac tctcgtctga aagaaaacgg ttttattaaa
     2161 aaccgcacca tctctcagct gtacgatccg gaaaaaggca tgttcctgcc ggaccgtttt
     2221 gtaaaaggca cctgcccgaa atgtaaatcc ccggatcaat acggcgataa ctgcgaagtc
     2281 tgcggcgcga cctacagccc gactgaactg atcgagccga aatcggtggt ttctggtgct
     2341 accccggtaa tgcgtgattc tgaacacttc ttctttgatc tgccctcttt cagcgaaatg
     2401 ctgcaggcat ggacccgcag cggtgcgttg caggagcagg tggcaaataa aatgcaggag
     2461 tggtttgaat ctggcctgca acagtgggat atctcccgcg acgcgcctta tttcggtttt
     2521 gaaattccga acgcgccggg caaatatttc tatgtctggc tggacgcacc gattggctat
     2581 atgggctctt tcaagaatct gtgtgacaag cgcggcgaca gcgtaagctt cgataaatac
     2641 tggaagaaag attccactgc cgagctgtat cacttcatcg gtaaagatat cgtttacttc
     2701 cacagcctgt tctggcctgc catgctggaa ggcagcaact tccgcaagcc gaccaacctg
     2761 tttgttcacg gctatgtgac ggtgaacggc gcgaaaatgt ccaagtctcg cggcaccttt
     2821 attaaagcca gcacctggct gaatcatttt gacgccgaca gcctgcgcta ctactacacc
     2881 gccaaactgt cttcgcgcat tgatgatatc gatctcaacc tggaagattt cgttcagcgc
     2941 gtgaatgccg atatcgtgaa caaagtggtt aacctggcgt cccgtaacgc tggctttatc
     3001 aataagcgtt ttgacggtgt gctggcaagc gagctggctg acccgcagtt gtacaaaacc
     3061 ttcactgatg ccgctgaagt gattggtgaa gcgtgggaaa gccgcgaatt tggtaaagct
     3121 atccgtgaaa tcatggcgct ggctgacctg gctaaccgct atgtcgatga acaggctccg
     3181 tgggtggtgg cgaagcagga aggtcgcgat gccgatctgc aggcgatttg ctcaatgggc
     3241 atcaacctgt tccgcgtgct gatgacttac ctgaaaccgg tactgccgaa actgaccgag
     3301 cgcgcagaag cattcctcaa tacagaactg acctgggatg gtatccagca accgctgttg
     3361 ggccataaag tgaatccatt caaggcgctg tataaccgca tcgatatgaa acaggttgaa
     3421 gcactggtgg aagcatccaa agaggaagta aaagccacag ccgcaccggt aactggccca
     3481 ctggctgacg acccgattca ggaaaccatc acctttgacg acttcgccaa agttgacctg
     3541 cgcgtggcgc tgattgaaaa cgcagagttt gttgaaggtt ctgacaaact gctgcgcctg
     3601 acgctggatc tcggcggtga aaaacgcaat gtcttctccg gcattcgttc tgcttacccg
     3661 gatccgcaag cactaattgg tcgtcacacc attatggtgg ctaacctggc accacgtaaa
     3721 atgcgcttcg gtatctccga aggcatggta atggctgctg gtcctggtgg gaaagatatc
     3781 ttcctgctaa gcccggatgc cggtgctaaa ccgggtcatc aggtgaaata atcccccttc
     3841 aaggcgctgc atcgacagcg ccttttcttt ataaattcct aaagttgttc tcttgcgatt
     3901 ttgtctctct ctaacccgca taaatactgg tagcatctgc attcaactgg ataaaattac
     3961 agggatgcag aatgagacac tttatctatc aggacgaaaa atcacataaa ttctgggcgg
     4021 tggagcaaca gggaaacgag ttgcatatca gttggggaaa agttggtacc caagggcaaa
     4081 gccaggtaaa aagttttgct gatgctgtgg cagcggaaaa agcggagctg aagctgattg
     4141 cggagaaggt gaagaaggga tatgtggagc aagcgaagga taattctttg caaccttccc
     4201 aaacggtaac ggactctctc aaggtagcgg acttatccac cattattcag gaacaaccct
     4261 ctttcgtagc agaaacccgt gcggctgaca aaaatacaga tgctgtttta ccgtggctgg
     4321 cgaaagatat tgctgtcgtt tttccgcccg aagttgtaca caccacgtta agtcatcgcc
     4381 gctttcccgg agttcctgtt cagcaagcag acaaattgac ccaattacgt cgcttagcct
     4441 gtagtgtgtc gcaacgggat aatacaacag ccacatttga cttcagcgcc tgttctttag
     4501 aatggcaaaa caccgtcgcc caggcgatca gtcagatcga cgacctgaaa acaacacagc
     4561 taccatcacc cgtaatggct gtactcacgg cacttgaaat gaaatgcaca agatataaag
     4621 agcgtgagga tgttatggat cagattattc aggaaggcgg tctggaatat gctactgatg
     4681 taataataca tcttcaacaa atatctattg aatgggatta tgtcaacaat aatattgttt
     4741 tcctgtcgtc tggcatttca cctgattatt tgcagcaata ttccagcttt gaattacgcc
     4801 tacgtaaaca tttatcactg gcggaagagt ctctctggca aaaatgtgca caaaaactta
     4861 ttgccgcagt tccacatatt ccagaatggc ggcaaccatt aattgctttg ttattgcccg
     4921 aaaaaccaga aattgcacat gaaattgccc agcgtctact ggggcaaaag aaattaccct
     4981 cgctggagtg gctaaaaata gtggcgactg atgagcacat tcttgcctca ttagaaaaat
     5041 atcacgagcc atatgccatt tttgatgatt actattgtgg tgcgatatgg tcagccaccg
     5101 tattacagga gcaaggtgtt gcggccctgc cccgatttgc tccctatgtc gcaagtgact
     5161 actgcgccga tgtgttgcgt catatcaatc atccgttcgc attaacactg cttatacgtg
     5221 tagcagggca tactaaacgc tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac
     5281 acgcagcact ggcagcactg gcggaacttc tagtgcaaaa agaagagaat agttggcgca
     5341 ttatgctaat gacgatgctt atctcgcaac cgacactagc agaacaggtc attccctggc
     5401 tttcgacacc cgcagttgca gtgctgaaat catgccagca acaactgaca cagccctcaa
     5461 accatgccag cgccgatcta ctgccggcca tagtagtctc cccaccatgg ctttcgaaaa
     5521 agaaaaaatc gccgattccg gtgctggatt tagcaccgct caacctcgaa tccatatgca
     5581 caataaccga cacagaagct aaagagtttc aaactcattg ggactgggaa ccgcataagc
     5641 caggtgaagg tgctaaaaat ttcctttact cgctggggta tcgccgctgg gattttgaca
     5701 catataaata tatcggcgct tctgactcgg caattgacgc atgggaacgt gaggacttcg
     5761 ctacactcat ccaaatgttt aaagcccacc atgcaccgta tcagggggag tggcatctca
     5821 actcacttcc ctttttacct atgcaaaaag cgattaagct atgggaattt ctcagcaaag
     5881 agccgcatac cgcgataaaa ccagtcatgc tttatttgcg actggcaggt atgagtggtt
     5941 ttctacactc gttttcacgc taccctcaag aaggttttgc ggttgctaat tattttgccg
     6001 caacggaact ggctcctgcc gtcgcccgcg ccttcaacaa actcaaaaca ttacgacaag
     6061 acgccagtag ctggctgctg aaatacccgg aacacgccat taccggcctg ctgcctgcag
     6121 cgctcggcaa agccagtgaa gcacaggaca acgcccgtgc tgccttgcgt atgcttaccg
     6181 aaaacggtca tcagccattg ctgcaagaaa tcgcccggcg ttataaccag ccggaagtaa
     6241 ccgatgcggt gaacgctcta cttgcgctcg atcccttaga taatcacccg acaaaaatcc
     6301 ccactcttcc gaccttttat cagccatcgc tctggacgcg cccgttatta aaagcaaacg
     6361 cccaatcact gcctgatagc gcactcctcc acctcggtga aatgctccgc ttccctcagg
     6421 aagaggcact gtatccggga ttattgcagg tgaaagatgc ctgtaccacc gactcactgg
     6481 ctgaatttgc ctgggatctg tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga
     6541 gttgggcgtt cactgcgtta ggcgttctcg gtaacgatga caccgcccgc aaactgacgc
     6601 ctttgattcg cgcctggcct ggtgaatccc agcataaacg cgccaccgtc gggttggata
     6661 ttctcgctgc tatcggtagt gatatcgccc tgatgcagct taacggcatc acccagaaac
     6721 tgaaattcaa agcattacag gagcgggcaa aagaaaaaat tgccgacatt gccgagagcc
     6781 gcgaactcac ggtggcggag cttgaagatc ggttagcacc ggatctcggt ctggatgata
     6841 atggttcgtt gttgctggat ttcggcccac gccagttcac cgtcagcttt gatgaaacct
     6901 taaaaccgtt tgtgcgtgac gcttccggca gtcgtctgaa agacctgccc aaaccaaaca
     6961 aaagcgatga tgaatcacag gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag
     7021 atgcgcgtac cgtcgccgcc cagcaggtag caaggctgga atccgccatg tgcctgcgtc
     7081 gccgctggtc accggaaaac tttcagctct tcctggttga gcatccgctg gtccgccact
     7141 taacccgccg tctgatttgg ggcgtttata gcgccgaaaa ccagctactg gcttgctttc
     7201 gcgtagcgga agataacagc tacagcaccg ctgacgatga tcttttcacc ctgccggaag
     7261 gcgatatctc tattggtatt cctcacgttc tggaaatatc accgacggat gctgccgcct
     7321 tcgggcagct ttttgccgac tacgaactgt taccaccgtt ccgccagctt gatcgtaaca
     7381 gttacgcgct gacagaagcc gagcgcaacg ccagcgaact gacacgctgg acaggcagaa
     7441 aatgcccgag tggtcgggta atggggctgg cgaataaagg ctggataaag ggcaccccgc
     7501 aggatgctgg ctggatcggc tggatgatca accccctggg ccgctggtcg ttaatcatgg
     7561 aaatcgatga aggttttgcg gtaggcatgt cgccagccga actgagtgcc gagcaactct
     7621 taagcaagct gtggctatgg gaaggcaaag cggaaagcta tggctggggg agtaattcaa
     7681 cacaggaagc gcagttctcc gtactcgatg ccatcaccgc cagcgagcta attaacgata
     7741 ttgaagccct gtttgaataa ggaaagagca tggacaagga attaccgtgg ctggcggata
     7801 acgcccaact ggaacttaaa tataaaaaag gcaaaacacc actcagccat cgcaattggc
     7861 caggcgaacc ggtgcctgtt atcactgaaa gcatcattca gacactgggt gatgaattgc
     7921 tacaaaaagc agagaagaaa aaaacattgt ctggcgttat gaaaactttt cactggagtg
     7981 gcagtccgcc atcacgcagg ccatcaactt gatcggcgaa cacaaaccct caatcacggc
     8041 ccggacaatg gcggcgctag cctgtatcgc gcaaaatgat agccaacagt tgctcgacga
     8101 aatcgtccaa caagaggggc tggaatatgc aactgaggtg gtgattgcac gccagtttat
     8161 tgtacggtgt tatgagagtg atcctctggt agtaacattg cagtatcaga acgaggacta
     8221 tggctatggt tatcgctcag aaacctataa cgaattcgat ctccgattac gtaagcatct
     8281 ctctctggca gaggaaacct gctggcagcg ttgcgccgac aaactcattg ccgcactgcc
     8341 aggaataacc aaagttcgcc gcccttttat tgctctgaac ctcccggaaa aacccgaaat
     8401 cgccaacgag ctggtaagcc ttgaatgttc gcaaactcat tttcgctcaa aagagtggtt
     8461 aaaagttgtt gctgatgacc ctaaggcggt gaaagaactc gcacgttact ggagccagga
     8521 tatatttagc gatcgagaag ccagctacat gtcgcatgaa aaccacttcg gctacgcggc
     8581 ctgcgctgcc cttttgcgcg aacaaggact ggcagccgtt ccgcgcctcg cgatgtatgc
     8641 acataaagaa gattgcggca gtctgctggt acaaattaac catccgcaag tcatccgcac
     8701 cttgctactg gtggctgata aaaacaaacc cagcctgcaa cgtgtggcta aatacagtaa
     8761 aaacttcccc catgcgacgc tcgccgcact ggcagaactg ctggcgttaa aagccccccc
     8821 tgcgcgccct ggttatccca tcatcgaaga caaaaagctg cctgcacagc aaaaagcacg
     8881 tgatgaatac tggcatacgc tgttacaaac gctgatggca tcgcagccac aactggcaga
     8941 agaggtgatg ccgtgcttaa gtacacaagc ccaggcagtg gtgaatggtt atttatcggc
     9001 atcgccaaaa cttgctttcg agagtactca tagcaacgac ctgccagaaa tacttgtttc
     9061 tccaccgtgg cgaggtaaga aaaaaacggc attgctgcgc cttgatttag tgcctcttga
     9121 attagcgccc aaagcccgct ggcaaccagg cgaacgagag agacttgccg ccacagagtc
     9181 tgcccgttat ttcagcacgg gatcttttac ggaacgcatg gaacgaaaaa gtggtcgagt
     9241 tgtactacag gagctaggtt ttggggatga tgtatggctg ttccggaatt atatactgcc
     9301 cggcaaactg gatgcggcgc gcaaatcatt agtgggtcag tggcattact ccccgcgcag
     9361 agtagaggaa atcaacaatg gctggcactc cacggaagca aaatcagccg aacaggcgct
     9421 tcgttccggc gacgttgaag cgttaattaa cacctgggaa aatgatagct actcacgtta
     9481 tcgtccggaa aagagtgtct ggaacctgta tttattggca cagttgccgc gtgagatggc
     9541 tttgactttc tggctgcgta tcaatgagaa aaagcatctg ttcgcgggtg aggactattt
     9601 tctcagtatc ctcgggttgg atacattacc tggcctgtta ttggcttttt cacatcatcc
     9661 aaaagaaaca tttccgttaa ttttaaattt cggcgcaaca gaactggccc tgcccgttgc
     9721 ccgcgtctgg cgacgtttcg cggcgcagcg tgatctggct cgccagtgga ttttacaatg
     9781 gccggaacat acggctactg cacttatccc tcttgtcttt acaaaaccca gcgataatag
     9841 cgaagctgca ttacttgccc tgcgtttact gtatgaacaa gggcatggcg aattactgca
     9901 aaccgtggca aaccgctggc agcgtacaga tgtatggcct gccctggagc aattacttaa
     9961 actgggtcca atagaaattt acccggcacg tattccaaaa gctcctgatt tctggcatcc
    10021 ggcaatgtgg tccaggccgc gccttatcaa gaataatcag cctgttaccg atgacgctct
    10081 ggaaattatc ggcgaaatgc tgcgctttac ccagggcgga cgtttttatg gcggactgga
    10141 acagctgaaa acgttttgcc agccgcaaac gctggcagct tttgcctggg atcttttcac
    10201 cgcgtggcaa caagctggtg ctcccgcaaa agacaactgg gcatttctgg cgttaagtct
    10261 ctttggcgac gaaagcacgg cacgggatct gacgacacag atcctcgcct ggccacaaga
    10321 gggcaaatct gcccgtgctg tcagtggtct gaatatactt actaagatga ataatgatat
    10381 ggcactgata cagctgcatt atatatcgca acgggcgaaa tcccgcccct tacgtgataa
    10441 cgcggcggaa tttcttcagg tggtcgcaga aaatcgcggg ctaagccagg aagagttagc
    10501 ggacagatta gtcccaacac tgggtcttga tgatccgcag gcgttgattt ttgattttgg
    10561 tccacggcaa tttaccgttc gcttcgatga aaacctcaac ccggttatct ttgatcagca
    10621 aaacgttcgc cagaaaagcg ttccccggtt gcgcgccgat gacgatcaac tgaaaacccc
    10681 cgaggcactg gcccgactca aagggctaaa aaaagatgcg actcaggtga gcaaaaacct
    10741 gctcccgcgt cttgaaactg ccctgcgcac cacccgacgc tggtcgctgg cagattttca
    10801 ttctctgttt gttaatcatc cctttacccg tctggttact cagcgattaa tatggggggt
    10861 ttatccggca aatgaaccgc gtcgtttact taacgccttt cgtgtggccg ctgaggggga
    10921 gttctgcaat gagcaagatg agccaattga cctgcctgcc gatgctctga ttggcattgc
    10981 ccacccgtta gaaatgacag cagaaatgcg cagtgaattt gcacagcttt ttgccgatta
    11041 cgaaattatg ccgccttttc gccagttgac gcggcgcaca gtgctgctca cgcctgacga
    11101 gtcagccagt aatagtttga atcgctggga aggtaaatct gctaccgttg ggcagcttat
    11161 gggaatgcga tacaaaggct gggagtcagg ctatgaggac gcatttgtct atgacttagg
    11221 tgcgtaccgg ctggtcctta agttttcacc cggttttaac cattacagta ctgatagcaa
    11281 agcgctaatg agcttccgtt ctcttcgggt ttaccgtgac aataaatccg tcacttttgc
    11341 cgaacttgat gtgtttgatt tgagtgaggc attaagcgca cctgacgtca ttttccatta
    11401 aaacacagcg ggcagtgatg caactgcccg ttatcaacac gacctaccag cgggggaagc
    11461 gatgatcgtg caaaaagagc tggttgctat ttacgattat gagatccctg tacctgaaga
    11521 gccgttttcc ttcagacttg aaatccataa atgctctgaa ttatttacag gttccgtcta
    11581 tcgactggag cgattccggc tacatccaac atttcatcaa cgtgatcgag aagatgccga
    11641 ccactaataa atgatgcgtt gatttatata agagatgagt ttattgatga gcggaaatta
    11701 cgaggtgaat cacatgaaac agtaatagca attttt
//
LOCUS       DADTFG010000146        11411 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.147, whole genome shotgun sequence.
ACCESSION   DADTFG010000146 DADTFG010000000
VERSION     DADTFG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.147"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            167..526
                     /locus_tag="J1138_003789"
     CDS             167..526
                     /locus_tag="J1138_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBA8455531.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITTRADLEGWNPGFTEKMVGWAKKMESGERIVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(646..825)
                     /locus_tag="J1138_003790"
     CDS             complement(646..825)
                     /locus_tag="J1138_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBA8455532.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            899..2260
                     /gene="pabB"
                     /locus_tag="J1138_003791"
     CDS             899..2260
                     /gene="pabB"
                     /locus_tag="J1138_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBA8455533.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTTINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2264..2842
                     /locus_tag="J1138_003792"
     CDS             2264..2842
                     /locus_tag="J1138_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBA8455534.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3026..4390
                     /locus_tag="J1138_003793"
     CDS             3026..4390
                     /locus_tag="J1138_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine dehydratase 1"
                     /protein_id="HBA8455535.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4521..6119
                     /locus_tag="J1138_003794"
     CDS             4521..6119
                     /locus_tag="J1138_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA8455536.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6123..7673)
                     /gene="yoaE"
                     /locus_tag="J1138_003795"
     CDS             complement(6123..7673)
                     /gene="yoaE"
                     /locus_tag="J1138_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBA8455537.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8136..9107
                     /gene="manX"
                     /locus_tag="J1138_003796"
     CDS             8136..9107
                     /gene="manX"
                     /locus_tag="J1138_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBA8455538.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9170..9970
                     /locus_tag="J1138_003797"
     CDS             9170..9970
                     /locus_tag="J1138_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBA8455539.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9983..10834
                     /locus_tag="J1138_003798"
     CDS             9983..10834
                     /locus_tag="J1138_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBA8455540.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10889..11347
                     /locus_tag="J1138_003799"
     CDS             10889..11347
                     /locus_tag="J1138_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBA8455541.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLAEDGVLVMQLEQRRLLIRVLNIDDLEKIYKLLVSTQ"
BASE COUNT         2792 a         2883 c         2868 g         2868 t
ORIGIN      
        1 gcgtctctgg ttttctttct tagctgaaca atttggttat agcgtgagaa ctgaccgtta
       61 attttctccc tcttactgga taacacacca tgagctgcta cctttaaaac acctgaatgt
      121 caacaggtta actcgtgcca tatcgttata atccctttga tattagatgc aaattaaggt
      181 catatatagc cttatcgata acatggttaa ttttaaggat aagaatatgc ctgctgtaat
      241 agataaagcc ctggatttca ttggtgccat ggatgtatca gcaccaacac caagttcgat
      301 gaatgaaagt acggcgaagg gaatctttaa atatttaaaa gaactgggag tacccgcgag
      361 tgccgctgat attacgacgc gagctgacct ggaaggctgg aacccggggt tcacggaaaa
      421 aatggttgga tgggcaaaaa aaatggagtc tggtgaacgc attgtgatta aaaatcctga
      481 atacttttca acatatatgc aggaagaact aaaagcactg gtctaagtta aatttacatc
      541 agcataaatg ggtcaggacg cttttaatca catatgaaaa gcgtcctttt tctcacaatc
      601 aaataaccat cggcttctat aacgaaagcg atacggataa gtgtgttact cgtcttcatc
      661 ctcaaaacgc gccacgattc gctcgccgga atggttggca cgcagctctt ctgccaccag
      721 cgcgattgcc tggccgctgc tcatcccttg agccatcagt tcctggatcc gctcgacagc
      781 tttttgctgc tgttcatggg tgagtgaagg taaacctgca aacattgtta actcctgcta
      841 aattgttggc gctaattatt tcatgctacc cggcacatag ccagtagagt caggactgat
      901 gaagacgtta tctcccgctg tgattacttt accctggcgt caggacgccg ctgaatttta
      961 tttctcacgc ttaagccacc tgccgtgggc gatgctttta cactccggct atgccgatca
     1021 tccatatagc cgctttgata ttgtggtcgc cgagccgatt tgcactttaa ccactttcgg
     1081 taaagaaacc gttgttagtg aaagcgaaaa acgcacaacg accactgatg acccgctaca
     1141 ggtgctccag caggtgctgg atcgcgcaga cattcgccca gcgcataacg aagatttgcc
     1201 atttcagggc ggcgcgctgg ggttgtttgg ctacgatctg ggccgccgtt ttgagtcact
     1261 gccagaaatt gcgcagcaag atatcgttct gccggatatg gcagtgggta tctacgactg
     1321 ggcgctggtt gtcgaccacc agcgtcaaac agtttctttg ctgagtcata atgatgtcaa
     1381 tgcccgtcgg gcctggctgg aaagccagca attctcgccg caggaagatt tcacgctcac
     1441 ttccgactgg caatccaata tgacccgcga gcagtacggc gaaaaatttc gccaggtaca
     1501 ggaatatctg cacagcggtg attgctatca ggtgaatctc gcccagcgtt ttcatgcgac
     1561 ctattctggc gatgaatggc aggcattcct tcagcttaat caggccaacc gcgcgccgtt
     1621 tagcgctttt ttacgccttg agcagggcgc gattttaagc ctttcaccag agcggtttat
     1681 cctttgtgat aatagtgaaa tccagacccg cccgattaaa ggcacgctac cacgcctgcc
     1741 cgatcctcag gaagatagca aacaagcaga gaaactggcg aactcagcga aagatcgtgc
     1801 cgaaaatctg atgattgtcg atttaatgcg taatgatatc ggtcgtgttg ccgtagcagg
     1861 ttcggtaaaa gtaccagagc tgttcgtggt ggaacccttc cctgccgtgc atcatctggt
     1921 cagcaccata acggcgcaac taccagaaca gttacacgcc agcgatctgc tgcgcgcggc
     1981 ttttcctggt ggctcaataa ccggggctcc gaaagtacgg gctatggaaa ttatcgacga
     2041 actggaaccg cagcgacgca atgcctggtg cggcagcatt ggctatttga gcttttgcgg
     2101 caacatggac accagcatta ctatccgcac gctgactacc attaacggac aaatttactg
     2161 ctctgcgggc ggtggaattg ttgccgatag ccaggaagaa gcggaatatc aggaaacttt
     2221 tgataaagtt aataagatat tacgccaact ggagaagtaa gacgtggaat accgtagcct
     2281 gacgcttgat gattttttat cgcgctttca acttttgcgc ccgcaaatta accgggaaac
     2341 cttaaatcat cgtcaggctg ccgtgttaat ccccatcgtc cgccgaccgc aaccggggtt
     2401 gttgctgact cagcgttcga ttcatctgcg taaacacgct ggacaagtgg cattccctgg
     2461 aggcgcagtc gatgacacgg acgcatcagt tatcgccgct gcgctgcgcg aagctgaaga
     2521 agaggtcgct ataccgcctt ccgccgttga ggttatcggc gtgctgccgc ccgtcgatag
     2581 cgtcactggc tatcaggtaa ccccagtggt cggcattatc ccgcccgatc tgccgtatcg
     2641 cgccagtgaa gatgaagtct ccgccgtctt tgaaatgccg ctcgcccagg cattacatct
     2701 gggtcgttat catcctttag atatctaccg ccgtggcgat tcacatcggg tatggctgtc
     2761 ctggtacgaa cagtattttg tatggggaat gaccgcaggc ataattcgtg agctggcgct
     2821 gcaaattggt gtgaaaccct gactatactt atctttacat ctacaaaaca ctacttgaga
     2881 caatcatcgc aatattagtt aaatcgcggt ttttgattag tttaattcat gtgaatagtt
     2941 aagccagtcg ccgcgttccc tcttacacta tgcgctgtta ttagttcgtt actggaagtc
     3001 cagtcacctt gtcaggagta ttatcgtgat tagtctattc gacatgttta aggtggggat
     3061 tggtccctca tcttcccata ccgtagggcc tatgaaggcg ggtaaacagt tcgtcgatga
     3121 tctggtcgaa aaaggcttac tggatagcgt tactcgcgtt gccgtggacg tttatggttc
     3181 actgtcgctg acgggtaaag gccaccacac cgatatcgcc attattatgg gtcttgcagg
     3241 taacgaacct gccaccgtgg atatcgacag tattcccggt tttattcgcg acgtagaaga
     3301 gcgcgaacgt ctgctgctgg cacagggacg gcatgaagtg gatttcccgc gcgacaacgg
     3361 gatgcgtttt cataacggca acctgccgct gcatgaaaac ggtatgcaaa tccacgccta
     3421 taacggcgat gaagtcgtct acagcaaaac ttattattcc atcggcggcg gttttatcgt
     3481 cgatgaagaa cactttggtc aggatgccgc caacgaagtg agcgtgccgt atccgttcaa
     3541 atctgccacc gaactgctcg cgtactgtaa tgaaaccggc tattcgctgt ctggtctcgc
     3601 tatgcagaac gaactggcgc tgcacagcaa gaaagagatc gacgagtatt tcgctcatgt
     3661 ctggcaaacc atgcaggcat gtatcgatcg cgggatgaac accgaaggcg tactgccagg
     3721 cccgctgcgc gtgccgcgtc gtgcgtctgc cctgcgccgg atgctggttt ccagcgataa
     3781 actgtctaac gatccaatga atgtcattga ctgggtaaac atgtttgcgc tggcagttaa
     3841 cgaagagaac gccgccggtg gtcgcgtggt aactgcgcca accaacggtg cttgtggcat
     3901 tgttccggca gtgctggctt actatgacca ctttattgag tccgtcagcc cggacatcta
     3961 tacccgctac tttatggcag cgggtgcgat tggtgcactg tataaaatga acgcctctat
     4021 ttccggtgcg gaagtcggtt gccagggcga agtgggtgtt gcctgttcaa tggctgctgc
     4081 gggccttgcc gagctgttgg gcggtagccc ggaacaggtt tgtgtggcag cggaaattgg
     4141 catggagcac aacctcggtc tgacctgcga ccctgttgca gggcaagttc aggtgccgtg
     4201 cattgaacgt aacgccattg cgtcagtcaa ggcgattaac gccgcgcgga tggccctgcg
     4261 tcgcaccagt gcaccgcgcg tctcgctgga taaggtcatc gaaacgatgt acgaaaccgg
     4321 taaggacatg aacgctaaat accgcgaaac ctcacgcggt ggtctggcaa tcaaagtcca
     4381 gtgtgactaa tacttcttat tcgcccatct gcaacggatg ggcgaattta ttcccgcttt
     4441 ctcgtctgct gtaattttcc ccactacact tccactgttg cgtcaggcgt ttgtcgccat
     4501 acgcttacag ggtggcccgc atgcaaaaag cacaacggat cattaaaacc tatcgccgta
     4561 atcgaatgat tgtttgtacg atttgcgcac tcgttacgct cgcttcgacc ctgagcgtgc
     4621 gatttatttc acagcgtaac ttaaatcaac aacgggtagt acaattcgcc aatcacgctg
     4681 tagaggaatt agataaagta ctgcttcccc tacaggcagg tagcgaagtc ttgcttccgc
     4741 tgattggtct gccctgctct gtcgcccatt tgccattacg taaacaggcg gcaaaactcc
     4801 aaactgtgcg atccattggc ctggtgcaag acggcacact ttattgctcc agcatttttg
     4861 gttatcgcaa tgtgcccgtc gtggacattc tggctgaact tcctgcaccg caaccacttt
     4921 tacgactgac gaccgaccgt gccctgataa aaggcagtcc ggttttgatt caatggacgc
     4981 ctgcagcggg cagtagcaat gctggggtca tggagatgat taacatcgac ttactgacgg
     5041 caatgctcct tgaaccacaa ctgccgcaaa tcagtagcgc cagcctgacg gtggacagtc
     5101 ggcatttgct ctatggtaat gggctggtag attcccttcc gcaacctgaa aacaatgaaa
     5161 actatcaggt ttcttcgcaa cgctttcctt ttaccattaa cgttaatggt ccgggggcta
     5221 cggcgctggc atggcactat cttccaacac aattaccgct ggcggtgctg ctaagtttac
     5281 ttgtgggcta tatcgcctgg ctggcgaccg cttaccgaat gagtttttcc cgcgaaatta
     5341 atctgggcct ggcgcaacat gagttcgaat tgttctgcca gcctttgctt aatgcgcgca
     5401 gccagcaatg tattggcgta gagattttgc tgcgctggaa caatccgcgt cagggctgga
     5461 tttcgccgga tgtgtttatt cctatcgcgg aagaacatca tttaattgtg ccactgaccc
     5521 gctatgtgat ggcagaaacc attcgtcagc gccatgtttt cccgatgagt agtcagtttc
     5581 atgttggcat taacgtcgca cccagccatt ttcgccgtgg tgtgctgata aaagatctca
     5641 atcagtactg gtttagcgct cacccgattc agcaactgat cctcgaaatc accgaacgcg
     5701 atgccttact ggatgttgat tatcggattg cccgcgagct acatcgtaaa aacgtcaaac
     5761 tggcgattga tgacttcggc accggcaata gttcattttc ctggcttgaa acattacgtc
     5821 ctgacgtgct gaaaattgat aagtcattta ccgcagctat aggttctgac gcggttaact
     5881 cgacggtgac cgatatcatc atcgcactgg ggcaaagact gaatattgaa ctggtggcgg
     5941 agggtgtgga aacgcaagaa caggcgaaat atttgcgccg tcatggcgtc catattttgc
     6001 aagggtattt gtacgcacag ccgatgccac tacgtgattt tcccaaatgg ctggcgggca
     6061 gccaaccgcc gcccgcccgg cataatggac atatcacccc cattatgccg ttacgttaac
     6121 gcttactcat cttcatcgtg cgccggttgc tctttaacaa tgcgaaccag atcaacacga
     6181 taatcattgg cttcaatgat ggtgatatgc agtggcccta catcaatcac atcgcccaca
     6241 cggggaatgt gaccatttgc cgagatcacg aggcccgcga ccgtcgcgat atcatcgtca
     6301 tcggcaaggt gctcaacatc aagcgcctgc tgcaaggcat gcaaatctgt accgcctttt
     6361 accagccagc cgtcaccgtc ggtaatgatt tccggcgttt cgtcagcgtc cgggaattca
     6421 cccgcaatgg cttccagcac atccagcggc gtgaccagac cttgtaccac accaaactcg
     6481 ttggtcacga taacaaagct cccgcgagca cgacgcagca cgcccagcag gttgatcgga
     6541 tcgagggttt ccgggacgat aatcgccgga gacgccgaag ctatcgccgc cacatcaact
     6601 ccctcttcca gcgccaccag cagttcttta gcacgtacga tgccgataat ttcatccagt
     6661 tcaccgcgac ataccgggaa caaactgtgc ggtgaggaga gcagttgctc gcggatttca
     6721 tcgaccccga gattagcatc aacccagctt atttcaccgc gcggcgtcat gatcccgcgc
     6781 agagaacgcg acgccagcgt cagtacgccg ttaatcatgt aacgttcttc ttcggcaaac
     6841 gcaccttccg ggatcggcat cgggttatcg gcatcgtgct gaacattggc ctgacgtttc
     6901 ccgcccatca aacgcaggat ggcatcggca gtacgcgctc gcagcggcaa agtcgactgg
     6961 tgacgaataa agttgcgacg cgcaatctgg ttaaacactt cgatgatgat cgagaagcca
     7021 atcgcggcat acaggtaacc tttcggaatg tggaaaccga aaccttctgc caccagactc
     7081 agaccaatca ttaacaggaa gctcagacag agcaccacca ccgtggggtg ctggttaacg
     7141 aatcgcgtca gcggttttga tgccagcagc ataaccgcca tcgcaatcac taccgccgcc
     7201 atcatcaccg gcaaatggtt aaccatccct actgcagtaa ttaccgcatc caacgagaag
     7261 acggcgtcaa ggatgacaat ctgtgtgacg accacccaga aactggcgta gcctttaccg
     7321 tggccggaat catgatcgcg gttttccagc cgttcatgca gttcagttgt tgctttgaac
     7381 agcaagaata tgcccccgaa caacataatc aggtcgcgtc cggagaagga gaaatccatg
     7441 acggtaaata gcggtttggt cagcgtgacc atccatgaaa tcagcgacag cagccccaga
     7501 cgcataatca gcgccagtga taatcccagc aaacgcgctt tatcgcgttg ttttggcggc
     7561 agtttgtcag caagaatggc gatgaagacc aggttatcga tacccagcac aatttcgaga
     7621 acaacaagcg tgagtagccc cgcccaaatc gaggggtcca ttaagaattc catgacaagc
     7681 tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc aggcgtaacg acaaaatgca
     7741 aacgaaaggt gcggcataaa gtgccagaaa ggcaggcgtt aaaaggcctg atgctgaaat
     7801 gacgtcggtg acgatccata ctgcgggcta ctgccctata ctccatggtt gttaaacagg
     7861 agttaaacat atcagagacg cctctgattt ggcaaagatt taccttcctt tgcaaacgaa
     7921 tgtgacaagg atattttacc tttcgaaatt tctgctaatc gaaagttaaa ttacggatct
     7981 tcatcacata aaataatttt ttcgatatct aaaataaatc gcgaaacgca ggggtttttg
     8041 gttgtagccc ttatctgaat cgattcgatt gtggacgacg attcaaaaac acatctggca
     8101 cgttgaggtg ttaacgataa taaaggaggt agcaagtgac cattgctatt gttataggca
     8161 cacatggttg ggctgcagag cagttgctta aaacggcaga aatgctgtta ggcgagcagg
     8221 aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc cgaaacgctg attgaaaagt
     8281 acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt gctgtttctc gttgatacat
     8341 ggggaggcag cccattcaat gctgccagcc gcattgtcgt cgacaaagag cattatgaag
     8401 tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt aatggcccga gatgatgacc
     8461 ctagctttga tgaactggtg gcactggcag tagaaacagg ccgtgaaggc gtgaaagcac
     8521 tgaaagccaa accgattgaa aaagccgcgc cagcacccgc tgccgcagca cccaaagtgg
     8581 ctccaactcc ggcaaaacca atgggaccga acgactacat ggttattggc cttgcgcgta
     8641 tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg gaccaaagaa accaatgtct
     8701 cccgtattat tgttgttagt gatgaagtgg ctgcggatac cgttcgtaag acactgctca
     8761 cccaggttgc acctccgggc gtaacagcac acgtagttga tgttgccaaa atgattcgcg
     8821 tctacaacaa cccgaaatat gctggcgaac gcgtaatgct gttatttacc aacccaacag
     8881 atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc tgttaacgtc ggtggtatgg
     8941 cattccgtca gggtaaaacc caggtgaata acgcggtttc ggttgatgaa aaagatatcg
     9001 aggcgttcaa gaaactgaat gcgcgcggta ttgagctgga agtccgtaag gtttccaccg
     9061 atccgaaact gaaaatgatg gatctgatca gcaaaatcga taagtaacgt attgtgttga
     9121 ttatcattca gttttcacac tttagtctta cgtaaacagg agaagtacaa tggagattac
     9181 cactcttcaa attgtgctgg tatttatcgt agcctgtatc gcaggtatgg gatcaatcct
     9241 cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc ctggtgggta tcgttcttgg
     9301 ggatatgaaa accggtatta ttatcggtgg tacgctggaa atgatcgcgc tgggctggat
     9361 gaacatcggt gctgccgttg cgcctgacgc cgccctggct tctatcattt ctaccattct
     9421 ggttatcgca ggtcatcaga gcattggtgc aggtatcgca ctggcaatcc ctctggccgc
     9481 tgcaggccag gtactgacca tcatcgttcg tactattacc gttgctttcc agcacgctgc
     9541 ggataaggca gctgataacg gcaacctgac agcaatttcc tggatccacg tttcttctct
     9601 gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc gttgcgctgt ctgttggtac
     9661 cagcgaagtc cagaacatgc tgaatgcgat tccggaagtg gtgaccaatg gtctgaatat
     9721 cgccggtggc atgatcgtgg tggttggtta tgcgatggtt atcaacatga tgcgtgctgg
     9781 ctacctgatg ccgttcttct acctcggctt cgtaaccgca gcattcacca actttaacct
     9841 ggttgctctg ggtgtgattg gtactgttat ggcagtgctc tacatccaac ttagcccgaa
     9901 atacaaccgc gtagccggtg cgcctgctca ggctgctggt aacaacgatc tcgataacga
     9961 actggactaa caggtgagcg aaatggttga tacaactcaa actaccaccg agaaaaaact
    10021 cactcaaagt gatattcgtg gcgtcttcct gcgttctaac ctctttcagg gttcatggaa
    10081 cttcgaacgt atgcaggcac tgggtttctg cttctctatg gtaccggcaa ttcgtcgcct
    10141 ctaccctgag aacaacgaag ctcgtaaaca ggctattcgc cgtcacctgg agttctttaa
    10201 cacccagcca ttcgtggctg cgccgattct cggcgtaacc ctggcactgg aagaacagcg
    10261 tgctaatggc gcagagatcg atgacggtgc tatcaacggc atcaaagtcg gtttgatggg
    10321 gccgctggct ggtgtaggcg acccgatctt ctggggaacg gtacgtccgg tatttgcagc
    10381 gctgggggct ggtatcgcga tgagcggcag cctgttaggt ccgctgctgt tcttcatcct
    10441 gtttaacctg gtgcgtctgg caacccgtta ctacggcgta gcgtatggtt actccaaagg
    10501 tatcgatatc gttaaagata tgggtggtgg cttcctgcaa aaactgacgg aaggggcgtc
    10561 tatcctcggc ctgtttgtca tgggggcgtt ggttaataag tggacacatg tcaacatccc
    10621 actggtggtc tctcgcatta ctgaccagac gggcaaagaa cacgttacca ctgtccagac
    10681 tattctggac cagttaatgc cgggcctggt accactgctg ctgacctttg cttgtatgtg
    10741 gctgttgcgc aaaaaagtta acccgctgtg gatcatcgtt ggcttcttcg tcatcggtat
    10801 cgctggttac gcttgcggcc tgctgggact gtaagactgt tgtacactac cggggccttt
    10861 tggccccgtt tttttatctg gaggattaat gacaatcacg gacctggtac tgattctttt
    10921 catcgccgca cttctggcct tcgcgatcta cgatcagttc atcatgcccc gccgtaacgg
    10981 ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc atcgatagcg ttatcttcgt
    11041 cggattgatt gtcattctta tctataacaa cgtcacgaat catggcgcgc ttataacaac
    11101 atggttatta agtgcactgg ctctgatggg tttttatata ttctggattc gcgttccgaa
    11161 gatcatcttt aaacaaaaag gttttttctt cgccaatgtc tggattgaat atagccgaat
    11221 caaagcgatg aacttggcgg aagatggcgt gttggtgatg caattagaac agcgtcggct
    11281 gttaatccgc gttctaaata tcgacgattt ggaaaaaatt tataagcttc tcgtttcaac
    11341 tcaataagtt atgaatttag ccaaagctat gtttagtgta tttttaataa tcagacatag
    11401 cttaggctat a
//
LOCUS       DADTFG010000147        11342 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.148, whole genome shotgun sequence.
ACCESSION   DADTFG010000147 DADTFG010000000
VERSION     DADTFG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.148"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            292..507
                     /gene="essD"
                     /locus_tag="J1138_003800"
     CDS             292..507
                     /gene="essD"
                     /locus_tag="J1138_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBA8455542.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            507..1004
                     /locus_tag="J1138_003801"
     CDS             507..1004
                     /locus_tag="J1138_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA8455543.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYITVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGSCDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1221..1403
                     /gene="rzoD"
                     /locus_tag="J1138_003802"
     CDS             1221..1403
                     /gene="rzoD"
                     /locus_tag="J1138_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HBA8455544.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1494..1787)
                     /gene="iss"
                     /locus_tag="J1138_003803"
     CDS             complement(1494..1787)
                     /gene="iss"
                     /locus_tag="J1138_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HBA8455545.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGSAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            2270..2596
                     /locus_tag="J1138_003804"
     CDS             2270..2596
                     /locus_tag="J1138_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HBA8455546.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            complement(2719..3072)
                     /locus_tag="J1138_003805"
     CDS             complement(2719..3072)
                     /locus_tag="J1138_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455547.1"
                     /translation="MPHTVHYPEQPSQGHVTPPPSRDAPVRFVLCCVAKTDNIRIYLV
                     FHDEFTQRLIEEGKMVSKNKAHCRRMLQALQQTRAGIFEQLENCQHTLPEYIAISSET
                     SATLIHRVPPEKKKK"
     gene            3548..4093
                     /locus_tag="J1138_003806"
     CDS             3548..4093
                     /locus_tag="J1138_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBA8455548.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEMLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            4068..5993
                     /locus_tag="J1138_003807"
     CDS             4068..5993
                     /locus_tag="J1138_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA8455549.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5990..6196
                     /locus_tag="J1138_003808"
     CDS             5990..6196
                     /locus_tag="J1138_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lambda prophage-derived head-to-tail joining
                     protein W"
                     /protein_id="HBA8455550.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            6193..7794
                     /locus_tag="J1138_003809"
     CDS             6193..7794
                     /locus_tag="J1138_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA8455551.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWMPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAHFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            7775..9091
                     /locus_tag="J1138_003810"
                     /pseudo
     CDS             7775..9091
                     /locus_tag="J1138_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
     gene            9101..9433
                     /locus_tag="J1138_003811"
     CDS             9101..9433
                     /locus_tag="J1138_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBA8455552.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            9495..9764
                     /locus_tag="J1138_003812"
     CDS             9495..9764
                     /locus_tag="J1138_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBA8455553.1"
                     /translation="MYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQIPG
                     LVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHLAWLFEAFV"
     gene            9897..11132
                     /locus_tag="J1138_003813"
     CDS             9897..11132
                     /locus_tag="J1138_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8455554.1"
                     /translation="MAGENKLSDKALKGYLGKPREKQITIADGKGLSIRVSTKGAVSF
                     VFFYRLAGGRAAPVWLTLGKYPDMSLKQAREKRDECRGWLADKRDPRIQIKIQAEERL
                     KPVTVEDALNYWYENYCKVRRKTHAVTLGRFRKHIFPYIGHLPVNDTHLYEWLDCFDR
                     IKRNAPVMAAYVFSDTKLALRFCRVRQYATCDVLKDLRMSDVGQIAGKRDRVLDEAEL
                     GQLWKAIFVEPDLKLMSEYTRKMFVLCTVFGCRMSEARLSEWSEWDLESWVWTVPKDH
                     SKTGVEIVRPVPEILRQWVTDVHEETKHTGYVLGSLRIRESVSKIGGKIGKRLGHEKQ
                     WSLHDLRRTLSTHLSDLGVEFYVVEQLLGHALPGVAGVYNRSKFMAKKLDALELWTTY
                     LNSIAGADSKVTILKQKVG"
     gene            11134..>11342
                     /locus_tag="J1138_003814"
     CDS             11134..>11342
                     /locus_tag="J1138_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBA8455555.1"
                     /translation="MKKMAIVDKKGLEYIPNIDRMIREKECRELTTLANSTRWKLEKE
                     GKFPKRIKIGSTAVAYRLSEVQAWIR"
BASE COUNT         2798 a         2688 c         3181 g         2675 t
ORIGIN      
        1 tgaagttttc ttatgcattt gtggtggtgt tttgaacact cggtggcatt ctcacaaata
       61 tcatttagta gtttacgtac gtaaaaaatt ggttatgctg ttaagagtgg ttacttcgtc
      121 acacagctta aacccgccgt cgagcgggtt tttccatttt ttgagtctcg atattagctg
      181 ataacccaat acctgagtta ttcactgact ccgaatctgt tacgtttctg cctttattgc
      241 gatacgtagt atccccttaa tttacacccg ctttgtctgc gaggtggggt tatgaaatcc
      301 atggataagt taacaacggg tgtcgcctat ggtacctcag caggtagtgc cgggtactgg
      361 tttttacagt tgctcgataa agtcacgccc tcacagtggg cggcaatagg tgtgctgggt
      421 agcctggtat ttggcctgct gacgtacctg acaaaccttt atttcaagat taaagaagat
      481 aagcgcaagg ctgcgagagg tgaataatgc ctccatcatt acgaaaagcc gttgctgctg
      541 ctattggtgg cggagcaatt gctatagcat cagtgttaat cactggccca agtggtaacg
      601 atggtctgga aggtgtcagc tacataccat acaaagatat tgttggtgta tggactgtat
      661 gtcacgggca tacaggaaaa gacatcatgc tcggtaaaac gtataccaaa gcagaatgca
      721 aagcactctt gaataaagac cttgccactg tcgccagaca aattaacccg tacatcacag
      781 tcgatatacc ggaaacaacg cgcggcgctc tttactcatt cgtttacaac gtgggtgctg
      841 gtaatttcag aacatcgacg cttcttcgca aaataaacca gggcgatatc aaaggctcat
      901 gtgatcagct acgtcgctgg acatatgctg gcggtaagca atggaaaggt ctcatgactc
      961 gtcgtgagat tgagcgtgaa atctgtttgt ggggtcagca atgaacagag taaccgcgat
     1021 tatctccgct ctggttatct gcatcatcgt ctgcctgtca tgggctgtta atcattaccg
     1081 tgataacgcc attacctaca aagcccagcg cgacaaaaat gccagagaac tgaagctggc
     1141 gaacgcggca attactgaca tgcagatgcg tcagcgtgat gttgctgcgc tcgatgcaaa
     1201 atacacgaag gagttagctg atgcgaaagc tgaaaatgat gctctgcgtg atgatgttgc
     1261 cgctggtcgt cgtcggttgc acatcaaagc agtctgtcag tcagtgcgtg aagccaccac
     1321 cgcctccggc gtggataatg cagcctcccc ccgactggca gacaccgctg aacgggatta
     1381 tttcaccctc agagagaggc tgatcactat gcaaaaacaa ctggaaggaa cccagaagta
     1441 tattaatgag cagtgcagat agagctgccc atatcgatgg gcaactcatg caattattgt
     1501 gagcaataca cacgcgcttc cagcggagta taaatgccta aagtaataaa accgagcaat
     1561 ccatttacga atgtttgctg ggtttctgtt ttaacaacat tttctgcgct gccacaaatt
     1621 ttggctgcat caacagtttt ctcctgtcca attcccgaaa cgaagaagtg atgggtgatg
     1681 gtttcctttg gtgttactgc tgtcggtttg tttccaacag taaacgtctg ttgagcacat
     1741 cctgtaataa gcattgccag agcggcagaa aacaacattt ttttcatctt attatcctgc
     1801 attgttaaaa acggcagaat cctatgtgac aacaattaaa cgatagttaa atggattgat
     1861 gaaaattaaa actatatagg tgtacgctca gactattgga ggaagttggg gacactcaga
     1921 atcctgtgga atgaaataaa ccggtctatc cgtccattac ccttttagct gcgctgtatc
     1981 gtcgccgtat tcccgcatta accatgaccg tagcccgacg gggaattcct tctgcgtgag
     2041 tgtgcgggaa taatcaaaaa cgatgcacac cgagttttta ccgcgttaat gattcgcggg
     2101 tttatcccgg tgcgatggtg gaagaaaccg gaagctgtat tacagaaagt gctactactg
     2161 tatcccgatg cgatgtatgt aatgtgagtc agataatggc acaggatgtg gtgatgtggc
     2221 agtctggaac acaggatata ttgtcagaat aagacccgta ggaataaaaa tgaaaagacg
     2281 ccttttacta ctttttctgt tatctgtcct ggcagtggga tgctcgcagc aaaaagctga
     2341 tgagccccgg caattagtga cggtgtatcc acgatatccg gaatatgctg cagcaaatta
     2401 tatcaaggga ctggttgagg ttaagttcga tattggtgct gatgggactg tgacacggat
     2461 cgtttttctc cgctcagagc ctcataattt gtttcgtgat gaagtggtga aggccatggc
     2521 gaaatggcga tttgaaaaga atcgtccctg tcagggagtg aagagacaat ttatctttac
     2581 gccgtcacgt ccctgatgct tccaggtaga gaggggctgg aagcaggaga aaaatgaaag
     2641 agccagcggt tatatttttg tcatggctga cgaggaatga tggaagaagg cgttgtatgc
     2701 cacacaacgc ctcactgttc atttcttctt tttctctggt ggaacccgat gaataagagt
     2761 tgcactggtt tccgatgaga tggcgatata ctcgggcaaa gtatgctggc agttttccaa
     2821 ctgctcaaaa atacctgctc tcgtctgttg caatgcctgc agcatgcggc ggcaatgcgc
     2881 cttgttttta ctaaccatct ttccttcctc tatcagtcgc tgcgtgaact catcatggaa
     2941 taccaggtaa atgcggatgt tatcggtttt ggctacgcag catagtacaa aacggacagg
     3001 tgcatcccgg gacgggggag gcgtcacatg tccctgtgat ggttgttccg ggtaatgcac
     3061 tgtgtggggc ataaaaatgt ccgataattt tactttctac cgcagttagt tgattcgttg
     3121 gtcctggtag cacattgggc gaggatttaa atgccaggca actgaaggat gatgttgcaa
     3181 gggagatagc gagaatattt ctgattttca tttgatgatg cctctgtgtg aaatgacggt
     3241 aaacgacgca cttgtgccgg cacataatag caagcaccat aatagatcag attcgattct
     3301 tgctgtaagt gataatcatt ctcgtttttg ggtcctttcc gtcgatccaa caggttacgg
     3361 ggcggcgacc tcgcggtttt tcactattta tgaaaatttt ccggtttaag gcgtttccgt
     3421 tcttcttcgc cgtaacctaa tgtttttatt taaaaccccc ctgaaaagaa aggaaacgac
     3481 aggtgctgaa aacgggcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga
     3541 atgaacaatg gaagtcaaca aaaagcagct ggctgacatt ttcggcgcga gtatccgtac
     3601 cattcagaac tggcaggagc agggaatgcc cgttctgcgg ggtggtggca agggtaatga
     3661 ggtactttat gactctgccg ccgccataaa atggtatgcc gaaagggatg ctgaaattga
     3721 gaacgaaatg ctgcgccggg aagttgaaga actgaggcag gacagcgaga cagatctcca
     3781 gccagggact attgagtacg aacgccatcg acttacgcgt gcgcaggccg acgcacagga
     3841 gctgaagaat gccagagact ccgctgaagt ggtggaaacc gcattctgta ctttcgtgct
     3901 gtcgcggatc gcaggtgaaa ttgccagtat tctcgacggg atccccctgt cggtgcagcg
     3961 gcgttttccg gaactggaaa accgacatgt tgatttcctg aaacgggata tcatcaaagc
     4021 catgaacaaa gcagccgcac tggatgaact gataccgggg ttgctgagtg aatatatcga
     4081 acagtcaggt taacaggctg cggcattttg tccgcgccgg gcttcgctca ctgttcaggc
     4141 cggagccaca gaccgccgtt gaatgggcgg atgccagtta ctatctcccg aaagaatccg
     4201 cataccagga agggcgctgg gaaacactgc cctttcagcg ggccatcatg aatgcgatgg
     4261 gcagcgacta catccgcgag gtgaatgtgg tgaagtctgc ccgtgttggt tattccaaaa
     4321 tgctgttggg tgtttatgcc tacttcatag agcataagca gcgcaacaca cttatctggt
     4381 tgccgacgga tggtgatgcc gagaacttta tgaaaaccca cgtcgagccg accatccgtg
     4441 atattccatc gctgctggcg ctggcaccgt ggtatggcaa aaagcaccgg gataacacgc
     4501 tcaccatgaa gcgtttcacc aatggtcgtg gcttctggtg cctgggcggt aaagcggcaa
     4561 aaaactaccg tgaaaagtcg gtggatgtgg cgggttatga tgaactcgct gcctttgatg
     4621 atgatattga acaggaaggc tctccgacgt tcctgggcga taagcgtatt gaaggctcgg
     4681 tctggccaaa gtccatccgt ggctccacgc ccaaagtgag aggcacctgc cagattgagc
     4741 gtgcagccag tgaatccccg cattttatgc gttttcatgt tgcctgcccg cactgcgggg
     4801 aggagcagta ccttaaattt ggcgataaag agacgccgtt tggcctcaaa tggacgccgg
     4861 atgatccctc cagcgtgttt tatctctgcg agcataatgc ctgcgtcatc cgccagcagg
     4921 agctggactt tactgatgcc cgttatatct gcgaaaagac cgggatctgg acccgtgatg
     4981 gcattctctg gttttcgtca tccggtgaag agattgagcc gcctgacagt gtgacctttc
     5041 acatctggac agcgtacagc ccgttcacca cctgggtgca gattgtcaaa gactggatga
     5101 aaacgaaagg ggatacggga aaacgtaaaa ccttcgtgaa caccacgctc ggtgagacgt
     5161 gggaagcgaa aatcggcgaa cgtccggatg ctgaagtgat ggcagagcgg aaagagcatt
     5221 attcagcgcc cgttcccgac cgtgtggctt acctgaccgc cggtatcgac tcccagctgg
     5281 accgctacga aatgcgcgta tggggatggg ggccgggtga ggaaagctgg ctgattgacc
     5341 ggcagattat tatgggccgc cacgatgatg aacagacgct gctgcgtgtg gatgaggcca
     5401 tcaataaaac ctatacccgc cggaatggtg cagaaatgtc ggtatcccgt atctgctggg
     5461 atattggcgg gattgacccg actattgtgt atgaacgctc gaaaaagcat gggctgttcc
     5521 gggtgatccc cattaaaggg gcatccgtct acggaaagcc ggtggccagc atgccacgta
     5581 agcgaaacaa aaacggggtt taccttaccg aaatcggtac ggataccgcg aaagagcaga
     5641 tttataaccg cttcacactg acgccggaag gggatgaacc gcttcccggt gccgttcact
     5701 tcccgaataa cccggatatt tttgatctga ccgaagcgca gcagctgact gctgaagagc
     5761 aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa aagcgacgca
     5821 atgaggcact cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt atttcccgct
     5881 ggcagctgga tcttagtgca ctgctggcga gcctgcagga agaggatggt gcagcaacca
     5941 acaagaaaac actggcagat tacgcccgtg ccttatccgg agaggatgaa tgacgcgaca
     6001 ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa aacgggtggc
     6061 aacggtacag aaagacggac ggagagtgga gtttacggcc acttccgtgt ctgacctgaa
     6121 aaaatacatt gcggagctgg aagtgcagac cggcatgaca cagcgacgca ggggacctgc
     6181 aggattttat gtatgaaaac gcccaccatt cccacccttc tggggccgga cggcatgaca
     6241 tcgctgcgcg aatatgccgg ttatcacggc ggtggcagcg gatttggagg gcagttgcgg
     6301 gcgtggaacc caccgagtga aagtgtggat gcagccctgt tgcccaactt tacccgtggc
     6361 aatgcccgcg cagacgatct ggtacgcaat aacggctatg ccgccaacgc catccagctg
     6421 catcaggatc atatcgtcgg atcttttttc cggctcagtc atcgcccaag ctggcgctat
     6481 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     6541 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     6601 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     6661 tgggatacca gtccctcgcg actgttccgg acacagttcc ggatggtcag cccgaagcgt
     6721 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     6781 gacagcggcg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     6841 cagaaatgga catggatgcc ccgtgagtta cccggcgggc gcgcctcgtt cattcacgtt
     6901 tttgaacccg tggaggacgg gcagacccgc ggtgcaaatg tgttttacag cgtgatggag
     6961 cagatgaaga tgctcgacac gctgcagaac acgcagctgc agagcgccat tgtgaaggcg
     7021 atgtatgccg ccaccattga aagtgagctg gatacgcagt cagcgatgga ttttattctg
     7081 ggcgcgaaca gtcaggagca gcgggaaagg ctgaccggct ggattggtga aattgccgcg
     7141 tattacgccg cagcaccggt ccggcttgga ggcgcaaaag tgccacacct gatgccgggt
     7201 gactcactga acctgcagac ggctcaggac acggataacg gctactccgt ttttgagcag
     7261 tcgctgctac ggtatatcgc agccgggctg ggtgtctcgt atgagcagct ttcccggaat
     7321 tacgcccaga tgagctactc cacggcacgg gccagcgcga acgagtcgtg ggcgcacttt
     7381 atggggcggc gaaaattcgt cgcatcccgt caggcgagcc agatgtttct gtgctggctg
     7441 gaagaggcca tcgttcgccg cgtggtgacg ttaccttcaa aagcgcgctt cagctttcag
     7501 gaagcccgca gtgcctgggg gaactgcgac tggataggct ccggtcgtat ggccatcgat
     7561 ggtctgaaag aagttcagga agcggtgatg ctgatagaag ccggactgag cacctacgag
     7621 aaagagtgcg cgaaacgcgg tgacgactat caggaaattt ttgcccagca ggtccgtgaa
     7681 acgatggagc gccgcgcagc tggtcttaaa ccgcccgcct gggcggctgc ggcatttgaa
     7741 tccgggctgc gacaatcaac agaggaggag aagagtgaca gcagagctgc gtaatctccc
     7801 gcatattgcc agcatggcct ttaatgagcc gctgatgctt gaacccgcct atgcgcgggt
     7861 tttcttttgt gcgcttgcag gccagcttgg gatcagccgc ctgacggatg cggtgtccgg
     7921 cgacagcctg actgcccagg aggcactcgc gacgctggca ttatccggtg atgatgacgg
     7981 accacgacag gcccgcagtt atcaggtcat gaacggcatc gccgtgctgc cggtgtccgg
     8041 cacgctggtc agccggacgc gggcgctgca gccgtactcg gggatgaccg gttacaacgg
     8101 cattatcgcc cgtctgcaac aggctgccag cgatccgatg gtgaacggca ttctgctcga
     8161 tatggacacg cccggcggga tggtggcggg ggcatttgac tgcgctgaca tcatcgcccg
     8221 tgtgcgtgac ataaaaccgg tatgggcgct ggccaacgac atgaactgca gtgcaggtca
     8281 gctgcttgcc agtgccgcct cccggcgtct ggtcacgcag accgcccgga caggctccat
     8341 cggcgtcatg atggctcaca gtaattacgg tgctgcgctg gagaaacagg gcgtggaaat
     8401 cacgctgatt tacagcggca gccataaggt ggatggcaac ccctacagcc atcttccgga
     8461 tgacgtccgg gagacactgc agtcccggat ggatgcaacc cgccggatgt ttgcgcagaa
     8521 ggtgtcggca tataccggcc tgtccgtgca ggctgtgctg gatacctagg ctgcagtgta
     8581 cagcggtcag gaggccattg atgccggact ggctgatgaa cttgttaaca gcaccgatgc
     8641 gatcaccgtc atgcgtgatg cactggatgc acgtaaatcc cgtctctcag gagggcgaat
     8701 gaccaaagag actcaatcaa caactgtttc agccactgcc tcgcaggctg acgttactga
     8761 cgtggtgcca gcgacggagg gcgacgccag cgcggcgcaa ccggacgtga acgcgcagat
     8821 caccgcagcg gttgcggcag aaaacagccg cattatgggg atcctcaact gtgaggaggc
     8881 tcacggacgc gaagaacagg cgcgcgttct ggcagaaacc cccggaatga ccgtggaaac
     8941 ggcccgccgc attctggccg cagcaccaca gagtgcacag gcgcgcagtg atactgcgct
     9001 ggatcgtctg atgcaggggg ctccggcacc gctggctgca ggtaacctgg catctgatgc
     9061 cgttaacgat ttgctgaaca caccagtgta agggatgttt atgacgagca aagaaacctt
     9121 tacccattac cagccgctgg gcaacagtga cccggctcat accgcaaccg cgcccggcgg
     9181 attgagtgcg aaagcgcctg caatgacccc gctgatgctg gacacctcca cccgtaagct
     9241 ggttgcgtgg gatggcacca ccgacggtgc tgccgttggc attcttgcgg ttgctgctga
     9301 ccagaccagc accacgctga cgttctacaa gtccggcacg ttccgttatg aggatgtgct
     9361 ctggccggag gctgccagcg acgagacgaa aaaacggacc gcgtttgccg gaacggcaat
     9421 cagcatcgtt taactttacc cttcatcact aaaggccgcc tgtgcggctt tttttacggg
     9481 atttttttat gtcgatgtac acaaccgccc agctgctggc ggcaaatgag cagaaattta
     9541 agtttgatcc gctgtttctg cgtctctttt tccgtgagag ctatcccttc accacggaga
     9601 aagtctatct ctcacaaatt ccgggactgg taaacatggc gctgtacgtt tcgccgattg
     9661 tttccggtga ggttatccgt tcccgtggcg gctccacctc tgaatttacg ccgggatatg
     9721 tcaagccgaa gcacttagca tggctttttg aggctttcgt gtagttgctg gtttttacac
     9781 ttaatctttt gataataaag aataagttta tctggcgctt tcactggatt ttcctcgtta
     9841 tctgtgtgtt gcaatcatct ctgtattgca gcttgtattg ctttttgggg ctaaaaatgg
     9901 ctggcgagaa caaactgagc gacaaagcac ttaaaggata tctggggaaa cccagagaaa
     9961 agcagatcac cattgctgat ggaaaggggc tttctattcg tgtgagtacc aagggggctg
    10021 tgagctttgt tttcttctac aggttagcag gtggccgggc tgctccggtc tggctaacgt
    10081 tgggtaaata tcctgatatg tcactcaaac aggcaaggga aaagcgcgac gagtgccgtg
    10141 gttggttggc tgacaaacgt gatccgcgta tccagattaa gattcaggct gaagaacgct
    10201 taaagccggt cacagtggag gatgcactaa attactggta tgaaaattac tgtaaggtgc
    10261 gtcgtaaaac tcatgctgta acgcttggca gatttcgaaa gcatatcttt ccctatatcg
    10321 gtcatttgcc cgtaaatgac actcacctat atgaatggct ggactgtttt gaccgaatta
    10381 aacgtaatgc accagttatg gcggcgtatg ttttttctga cactaaatta gctcttcgtt
    10441 tttgtcgggt acgccagtac gcgacgtgtg atgttttaaa ggatttgcgc atgagtgatg
    10501 tggggcagat tgcaggtaag cgggatcggg ttctggatga agccgaactc ggccagctct
    10561 ggaaggcaat ttttgtcgag cctgatttaa aactaatgtc tgaatacacg cgaaaaatgt
    10621 ttgtgctttg tacagtattt ggatgtcgaa tgagtgaagc ccgattatca gaatggagcg
    10681 aatgggatct cgaaagttgg gtttggactg taccaaaaga tcactcaaaa actggtgttg
    10741 aaatcgtcag accagtacct gaaattctac gacagtgggt aacggatgtt cacgaagaga
    10801 caaaacatac tggttatgtg ctgggaagtc tgcgaattag agaaagcgta agcaagattg
    10861 gggggaaaat cggtaaacgt ttgggccatg aaaaacaatg gtcactacac gaccttagaa
    10921 gaacgctatc tactcatcta agtgatctcg gtgttgaatt ttatgtagta gaacaactgt
    10981 taggccatgc gctacctggc gtggcgggtg tttacaaccg gagtaagttt atggctaaaa
    11041 aactggatgc tctggaactc tggactacat atctcaatag catcgcaggt gctgattcaa
    11101 aagtgacaat cctcaaacaa aaggttggtt aacatgaaaa aaatggcaat tgttgataaa
    11161 aagggtctgg agtacattcc taacattgac cgaatgatcc gtgagaaaga atgtcgggag
    11221 ctaaccactc ttgcgaacag cacacgctgg aagctggaga aggaagggaa atttcctaag
    11281 cggatcaaga ttggctccac tgctgttgca tatcgtcttt cagaagtgca ggcatggatt
    11341 cg
//
LOCUS       DADTFG010000148        11305 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.149, whole genome shotgun sequence.
ACCESSION   DADTFG010000148 DADTFG010000000
VERSION     DADTFG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.149"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(486..911)
                     /gene="arsC"
                     /locus_tag="J1138_003815"
     CDS             complement(486..911)
                     /gene="arsC"
                     /locus_tag="J1138_003815"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBA8455556.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEKLGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1246..1329
                     /locus_tag="J1138_003816"
     CDS             1246..1329
                     /locus_tag="J1138_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBA8455557.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1383..2735)
                     /gene="gorA"
                     /locus_tag="J1138_003817"
     CDS             complement(1383..2735)
                     /gene="gorA"
                     /locus_tag="J1138_003817"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBA8455558.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2807..3649)
                     /locus_tag="J1138_003818"
     CDS             complement(2807..3649)
                     /locus_tag="J1138_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HBA8455559.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3852..5894
                     /gene="prlC"
                     /locus_tag="J1138_003819"
     CDS             3852..5894
                     /gene="prlC"
                     /locus_tag="J1138_003819"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBA8455560.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERIVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENDELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5902..6654
                     /gene="rsmJ"
                     /locus_tag="J1138_003820"
     CDS             5902..6654
                     /gene="rsmJ"
                     /locus_tag="J1138_003820"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBA8455561.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6703..8172)
                     /gene="dtpB"
                     /locus_tag="J1138_003821"
     CDS             complement(6703..8172)
                     /gene="dtpB"
                     /locus_tag="J1138_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBA8455562.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8489..8923)
                     /gene="uspA"
                     /locus_tag="J1138_003822"
     CDS             complement(8489..8923)
                     /gene="uspA"
                     /locus_tag="J1138_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBA8455563.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9314..9649
                     /gene="uspB"
                     /locus_tag="J1138_003823"
     CDS             9314..9649
                     /gene="uspB"
                     /locus_tag="J1138_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBA8455564.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9720..11219)
                     /gene="pitA"
                     /locus_tag="J1138_003824"
     CDS             complement(9720..11219)
                     /gene="pitA"
                     /locus_tag="J1138_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBA8455565.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT         2838 a         2883 c         2988 g         2596 t
ORIGIN      
        1 cttttatttc catctggtaa tatttgagtg caaaaaaagg caataatcat atcgcaaaag
       61 aaaacgttaa caactatcga aaatgctcgt tttaaaaagg tatgatgttc tctgatagtg
      121 tgtgaagtga tatttttgtg atgttttaga tatgtaataa taaaataggt taacgtatgt
      181 tgaaattatt tattgtgtaa accactgata aatcataatc cagtacatct ttaaactgct
      241 gcactgtatc gctgataact aagcagctca ctgatacacc atgctggacg cgataattgg
      301 aatctcacgc agggagtact gtgtaaatag caaaatcgcc tgatacgcta cgactgtcag
      361 gcctacaaat aacatacaat tcatcgaatt atcataactt tgtaggtcgg ataaaacgcg
      421 ccagcgtcgc atccgacata aataaagcgc atttttctaa caacctgtgg gggaatatag
      481 ccgctttatt tcaggcgatt acccgcctca tcaaccactt tctcgccatc ttccttggtg
      541 aacgcgcctt tttgcgcatc tggcagaatt tccagcacga cttctgaagg gcggcacagg
      601 cgagttccct gtggcgtcac cacaatcggg cgattaatca gaatcgggtg ctgaagcata
      661 aagtcgatta actgatcgtg agtaaattta tcttctgcaa ggcccagttt ctcatacggc
      721 tcgacgtttt tacgcagcaa cgcgcgtacg gtaatcccca tatcggcaat gagttttacc
      781 agctcatcgc gcgtcggtgg tgtttccaga taatagataa tggtcggttc tgtaccgctg
      841 ttgcggatca tctccagcgt attacgcgac gtaccgcagg ccgggttgtg ataaatggta
      901 atgttgctca tatcagtatc tcattacgat gtgaaagaga ggtgtagcgc cagcgccacc
      961 agcgttacaa accaaagcgg acaagtcaaa aacacatatg gtttaacgaa tatgtaagtg
     1021 tagagaaagg tgtaggtgtg aattaagcgt tgggcattca tttgaaagga ggtctgaatc
     1081 aggacgtaaa aaaaccgact tcgcgtcggt ttttttactg ttcagccctg agttggtggc
     1141 tctggctgga gtgagatagc ccacatctaa caatgagaca tgcgccgtga caggcaatgg
     1201 atgagtaagc ggatgcattc tcactccatc gcatggagaa aacgggtgat tgataaagca
     1261 atcatcgttc ttggggcgtt aattgcgctg ctggaactga tccgctttct gcttcagctt
     1321 ctgaactgat agcggaaacg tagctaaggg ctaagagcac actactctta gccctttaac
     1381 atttaacgca ttgtcacgaa ctcttctgcc gctgtcgggt gaatggcaac cgtgttgtcg
     1441 aagtcttttt tggttgcacc catcttcagc gccaccgcga agccctgcaa catttcgtcc
     1501 ataccaaaac caatgccgtg aataccgaca atcttctctt ccggtccaac gcataccagt
     1561 ttcatgcgac acggctggcg gtgagtggtg acggcggtat acatcgcggt gaaagaggat
     1621 ttatacactt tcacctgatc gtcgccgtac tgctcgcgcg cctgcggctc cgttaaacca
     1681 acagtaccaa tcggcggatg gctgaagacc acggtcggaa tgttgctgta atccagatgc
     1741 tcatccggct tgttattaaa caggcgttca gagagacggc gacctgctgc aacagccacc
     1801 ggtgtcagtt ccactgcacc cgtgttatcg cccaccgcgt aaataccttc aacgttggtg
     1861 ttttgatatt tatcgacgac gatatagcct ttttcgttgg ttttaacgcc agcggcttcc
     1921 aggttgatgt tgtcattggc aggctcgcga ccaatcgccc aaattaggca atccaccgtt
     1981 tcactgcgac catcttccag ctccagcgtc aggctaccat cggcattttt cactaccgct
     2041 ttcgggatgg cgttggtgtg caactgcggg ccttcggcgt tcatcacttc aaccagcgtt
     2101 tcagagatca tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa cagatgcgtc
     2161 ttcgcaccga ggccgttaat cacgcccgcc agttcaacgg cgatgtaacc cgcgccaaca
     2221 accgctacgc gctctggcaa agccggaagg gcgaagaatc cgtcggaatc aataccgtat
     2281 tccacgcctg gaatatccgg gtggctcgga cgaccaccgg tggcgatcag aatatgatcg
     2341 gccgtgatgg tttcgccgtt gacttcaatg gttttggcat caacgaagcg ggcaaagcct
     2401 ttgattacat caacgttatt tttaccgagc acgttctcat aggaagtatg aatacggtcg
     2461 atataggcgg tacggctggc gatcaacgtt tcccagttga atttattgat agtggtatca
     2521 aaaccataat ccgggccgta catatggatc gcttcacgga tttgcgccgc gtgccacatc
     2581 acttttttcg gcacacagcc aacatttacg caggtgccgc ccagctcttt ggcttcaatc
     2641 agtgcgcatt tctggccgta catagccgcg cggttgatgg aggcgatacc gccgctgccg
     2701 ccgccgatgg cgatgtaatc atagtgttta gtcatgacat attgtcctta tcgttgatta
     2761 ccgcgattgt agcgcgagac gtaataggtg ccagcaatgg ctgcaattac tctggcacga
     2821 tccagcttac ggtggcgtgc ccggtgcctg ccggaaccag tttgctgtgc agccacggca
     2881 gcacgttatt catctgctgt tccagcttcc acggcgggtt aatcacaatc atgccagaag
     2941 cggtcatgcc acggcgatcg ctgtctggca gcaccgccag ttcaatttgc agaattttgc
     3001 gaatgccggt cgcttccagg tcgtggatca tgcgcttaat ttgctggcgc agcaccactg
     3061 gataccacaa tgcgtaagtg ccagtggcga aacgtttgta accttctgct atcccgctga
     3121 ccaccgcctg atagtcggtt ttcatttcat acggcgggtc gataaggatt aaaccacggc
     3181 gggaaaccgg cggcagcttg gccttaagct gctggaaacc gtcggctttt tcgacgcgcg
     3241 cacggctgtc tttctgaaat tcagaacgca gcaacgggta atcgctcggg tgcagttcgg
     3301 tcagttgcag gctgtcctgt tcacgcagta gctggcgagc aatcaacggc gaaccggggt
     3361 aataacgcaa ctgcccgcta cggttgaagt gttttaccac attgatgtac gcctccagct
     3421 ccgcaggcaa atcgtcctgc tgccagatac gggcgatgcc ttcgagatat tcgccggtac
     3481 gctcggcatg ttcgctgcct aactgataac gcccggcccc tgcgtgggtg tcgagataga
     3541 gaaacggttt atctttctct ttcagcgact caatgatcag gctctgaacg gtatgtttaa
     3601 ggacgtcggc gtggttgcca gcgtgaaagc tgtggcgata actgagcatg ggtaaaggtg
     3661 ttccggtaag taaaaatcgg cccgtattcg gggcgcacaa aagcctatca ggatagtata
     3721 ccgaaaagag gccgccgccg cgaaagcgta acgtttctca ttgaaattca ctacacttaa
     3781 ccccatgcta caaacattat gtaaagcgcc tgttgagcgc ttccttaacc tctttaacca
     3841 ggactgcgct aatgacgaat ccgttactga ctccctttga attgcctccg ttttctaaaa
     3901 ttctcccgga acatgtcgtt ccagccgtga ctaaggcgct gaacgactgc cgcgaaaatg
     3961 tggagcgcat agtagcgcaa ggggcaccgt acacctggga aaatctctgc cagccgttgg
     4021 cggaagtgga cgatgtgctg gggcgtatct tctccccggt cagccatctg aactcggtga
     4081 aaaatagccc ggaactgcgt gaagcgtacg aacaaaccct gccgctgctg tcagaataca
     4141 gcacctgggt agggcaacat gaagggctgt ataaggcata tcgcgacctg cgtgatggcg
     4201 atcattacgc cacgctgaac acggcgcaga aaaaagcggt tgataacgca ctgcgcgact
     4261 tcgaactctc tggcatcggt ctgccgaaag agaaacagca gcgttacggc gaaattgcta
     4321 cccgtctttc tgaactgggc aatcagtaca gcaacaacgt cctcgatgcg acgatgggct
     4381 ggaccaaact cgttaccgac gaagcggagc tggcggggat gccagaaagc gcgctggctg
     4441 cggcaaaagc ccaggccgaa gcgaaagagc tggaaggtta tttgctgacg ctggatatcc
     4501 caagctacct gccggtaatg acctactgcg acaaccaggc cctgcgtgaa gagatgtatc
     4561 gcgcttatag cactcgtgct tccgatcaag gtccgaacgc cggtaagtgg gacaacagca
     4621 aggtgatgga agagatcctc gcgctgcgtc acgaactggc gcaactgctg ggctttgaaa
     4681 actacgcctt caaatcactt gccactaaaa tggcagaaaa cccgcagcag gtgctggatt
     4741 tcttaaccga tctggcaaaa cgcgcgcgcc cgcaaggcga aaaagagctg gcgcaactgc
     4801 gcgccttcgc caaagccgaa tttggcgtcg atgagttaca accgtgggat atcgcttact
     4861 acagcgaaaa acagaaacag cacctctaca gcatcagcga tgaacaactg cgtccgtact
     4921 tcccggaaaa caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt tacggcatca
     4981 ccgctaaaga gcgtaaagat gttgatgtct ggcatccgga tgtacgtttc ttcgagctgt
     5041 atgacgagaa cgacgaactg cgcggcagct tctacctcga cctgtatgcc cgtgaaaaca
     5101 aacgcggcgg ggcgtggatg gatgactgcg taggccagat gcgtaaagcc gacggttcgc
     5161 tgcaaaaacc ggtcgcgtat ctgacctgca acttcaaccg cccggtaaat ggtaaaccgg
     5221 cgctgtttac ccatgacgaa gtgatcaccc tgttccacga gttcggtcac ggcctgcacc
     5281 atatgctgac ccgcattgaa accgctggtg tttccgggat cagtggggtg ccgtgggatg
     5341 cggtcgaact gccgagccag tttatggaaa actggtgctg ggagccggag gcgctggcgt
     5401 ttatctccgg tcactatgaa accggcgagc cgctgccgaa ggagctgctg gataaaatgc
     5461 tggcagcgaa aaattaccag gcggcgctgt ttattctgcg ccagctggag ttcggtctgt
     5521 tcgatttccg cctccatgcc gagttccgtc cggatcaggg ggcgaaaatc ctcgaaactc
     5581 tggcagaaat caagaaactg gttgccgtgg taccgtctcc gtcatggggc cgtttcccgc
     5641 atgctttcag ccacattttt gccggtggtt atgcggcagg ttactacagc tacctgtggg
     5701 ccgatgtgct ggcggcagat gccttctcgc gttttgagga agagggcatt ttcaaccgtg
     5761 aaactgggca atcgttcctc gacaacattc tgagccgtgg cggttcagaa gagccgatgg
     5821 agctgttcaa acgcttccgt ggtcgtgaac cacagctgga tgcgatgctg gagcattacg
     5881 gcattaaggg ctgaacattc agtgaaaatc tgcttaattg atgaaacagg cgccggagac
     5941 ggcgccttat ctgttctggc ggctcgctgg gggctggagc acgatgaaga caacctgatg
     6001 gcgctggtgt taacgccgga gcacctggaa ctgcgcaagc gtgatgagcc gaaacttggc
     6061 ggcatctttg ttgattttgt tggcggagcg atggcgcacc gacgcaaatt cggcggcgga
     6121 cgtggtgaag cggtggcaaa agcagtgggc attaaaggcg attatttgcc ggatgtggtg
     6181 gacgccaccg caggattagg gcgcgatgcc tttgtgctgg cttcggtcgg ctgccgcgtg
     6241 cggatgctgg agcgcaatcc ggtggttgcc gcgttgctcg acgatggtct ggcgcgtggt
     6301 tatgcggatg cggaaatcgg cggctggtta caggaacgtt tgcagttgat tcacgcctcc
     6361 agtctgacgg cgctgagcga tatcaccccg cgtccgcagg tagtttatct tgacccaatg
     6421 ttcccgcata agcagaaaag cgcgctggtg aaaaaagaga tgcgcgtgtt tcagtcgctg
     6481 gtggggccgg atcttgatgc cgatggattg ttggagcctg cgcggttgct ggcaaccaaa
     6541 cgcgtggtgg tgaagcgtcc ggactatgcg ccgccgctgg cgaatgtcgc cacgccaaac
     6601 gcggtagtca ctaaagggca tcgctttgat atttatgccg gaacgccggt gtaaaagtaa
     6661 agcggggtag cgataacgct accccgcttt ctgcaagaat aattaatggc tttccggcgt
     6721 cgcaatcatg cgtttcagcc acggcaccat caatagcatc actacggcaa cgcccagcgt
     6781 aaccaggcca atcttaccaa acacgttggt atagacgggc aacgtctcaa gtggatcggt
     6841 aatgttgtct ggtactgcgg taaatgttgc cacatagccg cccagcaaga acgcggcagc
     6901 ctgcgtcagg aaccacatcc cgagaataaa gcccatcaaa tgctgcggca ccagggcagc
     6961 aatcatcgcc aggccaaggg cgctaataaa cagttcacct aagctctgga ataagtacac
     7021 cagcacgata aaccatggcg atgtcagccc ctgggcatcc gcaaaccaca ttccagccgc
     7081 tgcagccgtc aaaaagccca gtgagcacat aaacatgccg agagtaaact tcatcggcat
     7141 cgagagatct ttgcctttgt tacccagatg cgtgtaaatg cctgccagta ttgggctggc
     7201 gagtaccacc cagaacgggt taagcgcctg gaagctgacc gggttgatgg aaaagccgag
     7261 aatttcatga tgcacgttgt tgatggcaaa gaagttcagc gacgttggca tctgggcgta
     7321 gagaatgtaa aacaccaccg cttcgagcat caggacaaag gcgacaaaca ttttattgcg
     7381 cccggtttta tccagcttga atgcctgacg aaagaagatg atggtgacga cgatggagag
     7441 aacaatcaga accagattgg cgacttctac gttgtgcatc agccaggcgc agacgaagat
     7501 catcaccacg ctgccaagta atacgtacaa cagtttactg aagctcatcg gccggaagtc
     7561 gggttcagaa ccaatgtctt tcaccattcc acgacaggcg atgtaaacca gtaatgcgat
     7621 aattaaccct gcaccgcaca ggttgtaggt gactgaatag ccgaatttat cagcgatcac
     7681 aggggccagc gataacgcta tcaacgagcc gatattgatc gacatataga acagggtgaa
     7741 tgcgccatca agccgcggat ctttcggcgg gtagcacttc gaaagcaagc tggctgggtt
     7801 agctttaaac aggccgttac cgacagcgat agtccccagg gcgatgaaaa tcaggtcagg
     7861 cttaagtagc gacatgccgg tcatgaagta gccaatcgcc agcacaagtg ctccgagaac
     7921 aatggtgcgt ttggttccca gcaggtggtc gccaacatag ccaccaatgg aaatgaggcc
     7981 atagaccagc gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg agaacccaag
     8041 ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga atcgctccca
     8101 taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc ccatgggtgt
     8161 tgttgtattc atatttatta accttccaat accatcctga aaagtattaa cgcgtaaggg
     8221 ataacgcgcg aaaaagttta catctgtcag agattcttaa tattgatgcg gagaatagat
     8281 taatacacta ttacaacaga aaataaccag ctgatcacac taatgatata aaatatgatg
     8341 gtgttaaatg ttgtattggc aggggttttg attgcgtacg tagaccgcag gccagataag
     8401 gcgtttacgc tgcatcaggt aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg
     8461 cgttcaggaa cacgtcgtgg agggaagatt attcttcttc gtcgcgcagc ggaacaatca
     8521 gcatatcaac gtgaacggtg ttgatcagct gacgtgcgga agacatcagt ttgctccaga
     8581 agtcctggtg gtgaccacaa accaccagat ccatatcgta tttcttgatt gcatcgacca
     8641 gtacctggcc cagatcgccg ctgccgctca gggtttcagt gattgggtag cctgcattgg
     8701 ttgaaagctc ggtcagtgca tgatgtgtct cttcagagat gcgtttctgc atatcaccca
     8761 gattcacatc gataagcccg gtgtataggt cagagtagtt tacatctacg tggatcagag
     8821 aaactttcgc attgtagggg cgagccatag agactgcttt ctctaccaga actttgcttt
     8881 ccggggagag gtcgaccgcg atgagaatgt gtttataagc catagtgtta ctccttccat
     8941 aaagttgtcg atgactggcc agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc
     9001 gaactacgtg cgcggggcta acccagccgt aaagattcag tgttaagacc atccttacct
     9061 tatagcgacc cggatgatcc gtcaatccgc cttgcttact cgttagataa acaaattttc
     9121 gacatgaata tattgatagt ggttaacctt ctggaaaaaa aacaacctga tctcctacac
     9181 tatctataga gccgctcgga tgttggtcac cacaaaagcg gtttctccag gacctggtgt
     9241 taactgattg gcagcgtatc gggagcggta gccccggggt ggaactccgc gggcaggtcg
     9301 ccggggagga ggtatgataa gcaccgtcgc attattttgg gctttatgtg tcgtttgcat
     9361 tgttaacatg gcgcgctatt tctcatcact acgcgcgttg ttagtggtac tgcgtaactg
     9421 cgatccattg ctctatcaat atgttgatgg agggggcttt tttacctcac atggccaacc
     9481 caacaaacag gtgcgtctcg tttggtatat ctatgcccaa cgttatcgcg atcatcacga
     9541 tgatgagttt attcgccgct gtgagcgggt gcgtcggcag tttattctga ccagcgcatt
     9601 gtgtggtctg gtggtggtca gcctgattgc attgatgatt tggcattaag caattcagca
     9661 ataatgacgc ataaaaaaag cgggccagta aactgacccg ctcgttttgg tgcgtacaat
     9721 tacaggaact gcaaggagag ccagtacagc ccgccggaaa gcagtaccgc agccggaagg
     9781 gtaaacaccc aggccatcag aatactggtt acggttttac gctgtaagcc gccgccatct
     9841 accaccatcg tccccgcgac agaagaggag agtacgtgag tagtggaaac cggcatcccg
     9901 gtataacttg ccaggccgat agacacagcc gccgtcatct gggcagacat cccctgagcg
     9961 taggtcatgc ctttcttacc gattttctca ccgatagtcg ttgccacacg acgccagcca
    10021 atcatcgtac cgatacctaa cgccagcgcg accgccatga tgatccacac cggcgcatac
    10081 tcgatggtgc taagcatgtc ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc
    10141 acgccaggca tcttcaccac tttatcgata gtgtcagaaa cgcacagcat aatgcggcgc
    10201 atctggctac gttgatcaag cgacagcttg tcgtagcttt ccacatcggt agtcaacatg
    10261 cctttcaggc ggttgagcgc gttaatggta ttcgacggat ggcaatggaa ctccgcgggt
    10321 tgcgttgcgc cagcttccgg agccggtact aactgatcag caccggtcgc ttgtttcagc
    10381 agcgcaggat gctgctcaaa gtaagcttcg acattgttga tggcatcacg ggtacgggtg
    10441 atttcgtagc cagtggcatt catgttcacc acgaagcctg ctggtgcgac accaatcaat
    10501 accaacataa ccagaccaat gcctttctga ccatcgttcg cgccgtgcga aaacgccacg
    10561 ccgatagcgg aaaggatcag cgcaatacgc gtccagaacg gcggcttttt cttgccgtct
    10621 ttcttttcac gctccgctgg ggtcaggtgg atacgggcgc gtttcttggt gccgctccag
    10681 tagcgacgca gcaagaaaat cagaccgcca gcaaacacca ggccgacaat aggggaaacg
    10741 atcagagaac cgaaaatact taatactttc gggatattga gtgcatccac cactgacgtc
    10801 ccggtcatca acgcattggt taaaccaatc ccgatgatcg cgccaatcag cgtatgagag
    10861 ctggatgcag gtaaaccaaa gtaccaggta cccaggttcc agataatcgc cgccagcaac
    10921 atagagaaca ccatggcaag gccatgagac gatcccatat taagcagcag atccgtcggc
    10981 agcatatgca caatggcata ggcaacactc agaccaccca gcaaaacacc caaaaagttg
    11041 aataccgccg ccataaccac ggcgagctga gaacgcatcg cgcgggtata gataacggtt
    11101 gccacggcgt tggctgtgtc atggaaacca ttgatggctt cgtagaacag cacaaaagcc
    11161 agtgcaagca ataataacag cccggtatgc aaatccaggc cagcaaacaa atgtagcata
    11221 ggacgttacg ccattttgag gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg
    11281 ggcaaagtga aatatagact ttttt
//
LOCUS       DADTFG010000149        11245 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.150, whole genome shotgun sequence.
ACCESSION   DADTFG010000149 DADTFG010000000
VERSION     DADTFG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.150"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(72..1436)
                     /gene="pgtP"
                     /locus_tag="J1138_003825"
     CDS             complement(72..1436)
                     /gene="pgtP"
                     /locus_tag="J1138_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter PgtP"
                     /protein_id="HBA8455566.1"
                     /translation="MLSIFKTGQSADSVPVEKIQVTYRRYRMQALLSVFLGYLAYYIV
                     RNNFTLSTPYLKEQLDLSATQIGVLSSCMLIAYGISKGVMSSLADKASPKVFMACGLV
                     LCAIVNVGLGFSTAFWIFAALVVLNGLFQGMGVGPSFITIANWFPRRERGRVGAFWNI
                     SHNVGGGIVAPIVGAAFALLGSEHWQGASYIVPACVAIVFAVIVLILGKGSPRQEGLP
                     SLEEMMPEEKVVLNTRQTVKAPENMSAFQIFCTYVLRNKNAWYVSLVDVFVYMVRFGM
                     ISWLPIYLLTVKHFSKEQMSVAFLFFEWAAIPSTLLAGWLSDKLFKGRRMPLAMICMA
                     LIFICLIGYWKSESLFMVTIFAAIVGCLIYVPQFLASVQTMEIVPSFAVGSAVGLRGF
                     MSYIFGASLGTSLFGIMVDHIGWHGGFYLLGCGIICCIIFCWLSHRGAIELERHRAAY
                     IKEH"
     gene            1835..3052
                     /gene="pgtC"
                     /locus_tag="J1138_003826"
     CDS             1835..3052
                     /gene="pgtC"
                     /locus_tag="J1138_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transport regulator PgtC"
                     /protein_id="HBA8455567.1"
                     /translation="MILLLLLMDAGAVRAQRNELVMATTFSPGATAWIIQRWQTEPES
                     VMIRTLNRTSASLEQLLDTANVENVDLILTSSPMLLQHLQEHQKLAPFDDAPAESQNL
                     VPESIRATSVAVAISGFGLLINRPALSVKHLPAPADWDDLALPIYQDALLMSSPSRSD
                     TNHLMVESLLQQKGWVKGWETLLTSAGNLVTISSRSFGVADKIKSGLGVAGPVIDNYA
                     NLLLNDPHLSFTYFPRSAVSPTYVAILKKSPHADAARRFIHYLLSPKGQRILADANTG
                     KYPVTPLAADNPRVTQQQLLMNQPPLNYHLILKRQPLVQRLFDTAISFRLAQLKDAWR
                     ALHSTEARLKKPLPEIRALLTQVPVAAASSEDPVWLAQFDNKSFAEQQMMEWQLWFLN
                     NQRLAIKKLEELK"
     gene            3049..5058
                     /gene="pgtB"
                     /locus_tag="J1138_003827"
     CDS             3049..5058
                     /gene="pgtB"
                     /locus_tag="J1138_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004137022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PgtB"
                     /protein_id="HBA8455568.1"
                     /translation="MKNITLWQRLRQVSISTSLRCAFLMGALLTLIVSSVSLYSWHEQ
                     SSQIRYSLDEYFPRIHSAFLIEGNLNLVVDQLNEFLQAPNTTVRLQLRTQIIQHLDTI
                     ERLSRGLSSRERQQLTVILQDSRSLLSELDRALYNMFLLREKVSELSARIDWLHDDFT
                     TELNSLVQDFTWQQGTLLDQIASRLGDTAQYLKRSREVQNEQQQVYTLARIENQIVDD
                     LRDRLNELKSGRDDDIQVETHIRYFENLKKTADENIRMLDDWPGTITLRQTIDELLDM
                     GIVKNKMPATMREYVTAQKALEDASRTREATLGRFRTLLEAQLGSTHQQMQMFNQRME
                     QIVRVSGGLILVATALALLLAWVFNHYFIRSRLVKRFTLLNQAVVQIGLGGTETAIPV
                     YGNDELGRIAGLLRHTLGQLNVQKQQLEQEITDRKVIEADLRATQDELIQTAKLAVVG
                     QTMTTLAHEINQPLNALSMYLFTARRAIEQTQTEQASMMLGKAEGVIGRIDAIIRSLR
                     QFTRRAELEISLHSVDLAQMFSAAWELLAMRHRPLQATLVLPQGTATVSGDEVRTQQV
                     LVNVLANALDVCGQGAVITVNWQMQGKTLNVFIGDNGPGWPEALLPSLLKPFTTSKEV
                     GLGIGLSICVSLMEQMKGELRLASTMTRNACVVLQFRLTDVEDAK"
     gene            5048..6295
                     /gene="pgtA"
                     /locus_tag="J1138_003828"
     CDS             5048..6295
                     /gene="pgtA"
                     /locus_tag="J1138_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PgtA"
                     /protein_id="HBA8455569.1"
                     /translation="MLSSEYSILLIDDDADVLDAYTQLLEQSGYRVFACNNPFEAQTW
                     IQPDWPGIVLSDVCMPGCSGIDLMMLFHQDDQQIPILLITGHGDVPMAVDAVKKGAWD
                     FLQKPVDPGKLLSLVEEALRQRQSIIARRQYCQQTLQVELIGRSEWINQYRRRLQQLS
                     ETDIAVWLYGAPGTGRMTGARYLHQFGRNAQGEFIYRELTPDNAPQLNDFIALAQGGT
                     LVLSHPEHLTREQQYHLVQLQSQEHRPFRLIGIGDTSLVELAASNHIIAELYYCFAMT
                     QIACLPLTQRPDDIEPLFRHYLCKACQRLNHPVPEVGKEMLKEMMRRMWPNNVRELAN
                     AAELFTVGVLPLAETANPLMHVGTPTPLDRRVEDAERQIITEALNIHQGRINEVAEYL
                     QIPRKKLYLRMKKYGLSKEHYKI"
     gene            complement(6382..7080)
                     /locus_tag="J1138_003829"
     CDS             complement(6382..7080)
                     /locus_tag="J1138_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute-binding protein"
                     /protein_id="HBA8455570.1"
                     /translation="MRVLAAGSLRRVWKSLVSEYQADNIQCDFGPAGILRERIEAGEA
                     CDFFASANMTHPQILMSAGRALCIKPFASNRLCLYVRANKFNENDDWYSLLNRETLRI
                     GTSTAGCDPSGDYTQELFENMGSVGEKIRQRAVALVGGRHSLPLPGNAIAAQWLIEND
                     YTDLFIGYANYVPGLQSIDSVKVIEIPEPYNPIAIYGFACLTDKALPLADFLVSPVAR
                     GILEQHGFMPPGTL"
     gene            complement(7080..8132)
                     /locus_tag="J1138_003830"
     CDS             complement(7080..8132)
                     /locus_tag="J1138_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA8455571.1"
                     /translation="MKINNKLFLLLIFLLSLSFTTQAERTFTDQIGREVKVPDKVDRI
                     VVLQHQTLNLLVQMNATDKIVGVMSNWKQQLGSNYARLAPELSNKASLGDLTHVDAEK
                     LVALHPQVVFVTNYAPQEMIDKISSLGIPAVAISLRHDDEGERNKLNPVMADEEQAYV
                     KGLYEGIMLIGNIINKPEEAKALIKATENGRRMVSNRLQLLPEEQRVRAYMANPELTT
                     YGSGKYTGLMMKHAGAVNVAASTIKGFKQVSIEQVIEWNPQVIFVQNRYPAVVNEIQS
                     SPQWQVIDAVKNHCVYLMPEYAKAWGYPMPEAMGIGELWMAKKLYPEKFNDVDMHKIV
                     NGWYRTFYRTDYQGED"
     gene            8342..9409
                     /locus_tag="J1138_003831"
     CDS             8342..9409
                     /locus_tag="J1138_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA8455572.1"
                     /translation="MPHPIHSPSPETIKYQYHQLVHKRIILISIITLAMLSSLFLDII
                     LGSSNLPVSELWHALAHPFSRDTSVHVIVWEIRLPYALMALLVGMTLGLAGAEMQTIL
                     NNPLASPFTLGVSSAAAFGAALAIVLGIGIPGISAAWFVPANAFIFALLSSLLLDSMS
                     RWLRIASSGMILLGIALVFTFNALVAIMQFIADEDTLQGLVFWTMGSLARASWNRLGI
                     LSGVFIIVFLWSFSRSWQLTALRMGEERALSFGINAQQLRLSSLLRISLLAALSVAFV
                     GPIGFIGLVSPHISRMLFGEEHRFYLTGSVLTGGLVLSLASIASKNIISGVIIPIGIV
                     TSLVGVPLFISIILRYRRSLL"
     gene            9406..10179
                     /locus_tag="J1138_003832"
     CDS             9406..10179
                     /locus_tag="J1138_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8455573.1"
                     /translation="MKGLSLHKLNAGYQKKNVICDLSLPELQRGKITIILGPNGCGKS
                     TLLRALAGLNKANGKVFLDGVNLMQMPFSARADSTIFLPQFLPHDVHLNVLESILVSL
                     RASKMGGKHEKTQSIAILKQLGIEHLAFCYLDQLSGGQRQLVGLAQSLVRNPSILLLD
                     EPLSALDLNHQFHVMDLVKSETRNRNIVTIMVVHDLNIALRYGDYVVMLKKGKLIASG
                     KPKEVITPENLANVYRIRGRIEHCSHGLLQLIVDGVIPD"
     gene            complement(10418..11032)
                     /locus_tag="J1138_003833"
     CDS             complement(10418..11032)
                     /locus_tag="J1138_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA8455574.1"
                     /translation="MASERKKAIVIGAYSAFIELGFAQTSTTEIARRAKVSKRTIYEV
                     FSNKKEIFSEVIKEYKALFLDLPRPENEELPLNEALFRIFRLDISQEDAKEREALLKL
                     MTRESILFPDLSDYLYTTKTLRSRELLIEWLDIQQAQGRITVKNTGNCVGMLMDIVFG
                     ALIPRRWVLDLNEDQTLTPEGRQQQLEEIKERIETVIQVIKNSI"
     gene            11025..11245
                     /locus_tag="J1138_003834"
                     /pseudo
     CDS             11025..11245
                     /locus_tag="J1138_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3143 a         2616 c         2479 g         3007 t
ORIGIN      
        1 ctctgatttc cagatatctt ataaatcata atatactcca ctccccaagg agacggccct
       61 ggggaaggta atcagtgttc ttttatatat gcggctctgt gacgttcaag ttcaattgca
      121 ccacgatgtg ataaccagca gaaaatgatg caacaaataa taccgcagcc aagaagataa
      181 aatccgccat gccagccaat atgatcgacc ataataccaa acaggctggt gcccagagac
      241 gcaccgaaga tatagctcat aaaaccgcgt aagcctactg cagaaccaac agcaaagctg
      301 ggaacgatct ccatagtctg aacggaagcc agaaattgtg gaacgtaaat caggcaacca
      361 acaatggcag caaaaattgt caccataaac agcgattcac ttttccagta gccaatcagg
      421 caaatgaaaa tcagcgccat acaaatcatc gccaatggca tacgacgccc tttaaacagt
      481 ttgtctgaca accaaccggc aagtagcgtg gaagggattg cagcccattc aaaaaataaa
      541 aacgcgacgc tcatttgttc tttagaaaaa tgtttcaccg tcagcaggta aataggcaac
      601 cagctaatca tcccgaagcg caccatgtat acaaatacgt caaccagtga gacataccag
      661 gcatttttgt tgcgtaatac ataagtgcag aaaatctgaa aggcgctcat gttttctggt
      721 gcttttaccg tctgtcgggt attcaggacg actttttctt ccggcatcat ctcttccaga
      781 gagggtagac cttcctgacg tggggaacct ttaccgagaa tcagcacaat taccgcaaaa
      841 acgatagcca cgcaggccgg aacgatatag ctcgcacctt gccagtgctc gctgccgagt
      901 agggcaaaag cggcaccaac aataggggca acaataccac caccgacgtt atgagagata
      961 ttccagaaag caccaacccg accacgctcc cggcgaggga accagttagc aatagtgatg
     1021 aaagaaggac caacgcccat tccctggaaa agaccattca gaacaaccaa tgccgcaaaa
     1081 atccagaatg cagtgctgaa tcccaggcca acgttaacga tggcacataa caccagccca
     1141 cacgccataa agactttcgg actggcttta tcggcaaggc tactcatcac tcctttgctg
     1201 ataccatagg cgataagcat acagctactc agtacgccaa tttgtgtggc gctgagatct
     1261 aattgctctt taagataagg cgtcgataaa gtgaaattat tacgcacgat atagtatgca
     1321 agatacccca gaaatacgct aagtaacgcc tgcatacgat agcgacgata tgtcacctga
     1381 attttctcca ccggaacact atccgccgat tgccccgttt taaatattga taacatgtct
     1441 tcacctgtat tttatattga tatgaacatc aggagtattc tgacaatgag agtcatataa
     1501 ttgagatgaa acatcaaact gaaaaaatgt gtcacaattg acacaaaata ataaaatcaa
     1561 agcctgtttt tgacccattt tattgatgca tagacaaggt agatttatgc tttatctggt
     1621 ttgttgttag catgtgcgga tattaacaaa tctcatgctg attatcgggt aatctctgat
     1681 taatatatta acggtctctt tgagcagacg tgttatcaga cgaagtgtct gaaacgggta
     1741 atttttgtct caatgcgatt tttgcggtgt attaatattt atgacctgga tcacaaaccg
     1801 attttttagt tggtgcgaaa tcggcatcaa aaccatgatt cttttactgt tgctgatgga
     1861 cgccggggcc gtcagggctc aacgaaatga actggtgatg gccaccacat tctcacccgg
     1921 agctaccgcg tggataatac agcgctggca aacagagcct gagtcggtaa tgatccgtac
     1981 gcttaaccgc accagtgcct cactggaaca gttgcttgat acggccaacg tagaaaacgt
     2041 cgatcttatc ctgacttcat caccaatgct gctccagcac cttcaggagc accagaaact
     2101 ggccccgttt gatgatgcac ccgcagaaag ccaaaacctg gtgccggagt cgatccgtgc
     2161 aacctccgtt gcagtagcaa tatcaggttt tggtctgctc attaatcgtc cggcgctttc
     2221 tgtaaaacac cttcctgccc ctgctgactg ggacgatctt gctttgccga tctatcagga
     2281 cgctttattg atgagtagtc cgtcgcgttc agatactaac catttaatgg ttgagtcatt
     2341 actacagcaa aaaggctggg tgaaaggatg ggaaacgttg ctgacaagtg caggaaacct
     2401 ggtgacaatc tcctcccgca gttttggcgt agccgataaa ataaaaagcg gactcggcgt
     2461 tgccggcccc gttatcgata actatgctaa tttactgtta aatgaccccc atctctcttt
     2521 tacctatttc ccccggtctg cggtatcccc cacctacgtt gcaattctca agaaaagccc
     2581 acacgccgat gcagcccgtc gctttattca ctacctgtta agtccgaaag gacagcgtat
     2641 tctggccgat gccaatacag gaaaatatcc ggtgacacca cttgccgcag acaatccgcg
     2701 ggtaacgcaa caacagctcc tgatgaatca acccccgctg aattatcacc ttatcctgaa
     2761 acgtcagcct ctggtgcagc gcttgtttga tacggcaatt agctttcggt tagcacagct
     2821 aaaagatgcc tggcgagcgc tgcacagtac cgaggcacgt ctgaagaaac cattaccaga
     2881 aatacgtgca ctgttaacac aagtgcccgt tgcagcggcc agtagtgaag atcccgtctg
     2941 gctggcacag ttcgataaca aaagctttgc cgagcagcaa atgatggaat ggcaactctg
     3001 gtttctgaat aaccagcgcc tggcgataaa aaaactggaa gagctgaaat gaaaaacata
     3061 acgctgtggc agcgtttaag acaggtcagt atcagtacca gcttacgttg cgcatttctg
     3121 atgggggcac ttctgaccct gattgtcagt agtgtcagtc tgtattcatg gcatgaacaa
     3181 agctcacaaa ttcgttactc gctggatgag tattttcccc gtattcactc tgctttcctt
     3241 attgaaggga acctgaatct ggtggtagac caactaaatg aatttttgca ggctcccaac
     3301 accacggtgc gattgcaact tcgtacccag attattcagc atctcgacac catagaacgg
     3361 cttagtcggg gactgtcatc ccgggaacgc caacaactga cggtcatttt gcaggacagt
     3421 cgatcactgt tatccgagtt ggatcgtgcg ctttacaaca tgtttttact acgggaaaag
     3481 gtgagtgagc tatcagcgcg gattgactgg ttacacgatg attttactac cgagcttaat
     3541 tctttagtgc aggatttcac ctggcagcag ggaacgctgc tggatcaaat cgcctcccga
     3601 ctgggcgata cggcgcaata cctgaagcga tctcgtgaag tgcaaaatga acagcagcag
     3661 gtttataccc tggcacgcat tgaaaatcag attgttgacg atctgcgtga cagactcaat
     3721 gagctcaaat caggacgtga tgacgacata caggtggaaa ctcatatccg ttattttgaa
     3781 aatctgaaaa aaacggcaga tgaaaatata cgtatgctgg atgactggcc tggcaccatt
     3841 accctgaggc agaccatcga tgaattgctg gatatgggaa tcgtaaaaaa caaaatgccg
     3901 gctacgatgc gtgaatatgt caccgcccaa aaagccttag aggatgccag tcgcaccagg
     3961 gaagcgacac tgggtcgctt cagaacgtta ctggaagcgc agcttggcag tactcatcaa
     4021 caaatgcaga tgtttaatca acgaatggaa caaattgttc gcgttagcgg tgggctgatc
     4081 ctggtggcga cagcactggc gttactgctg gcatgggtat tcaaccatta ttttatccgc
     4141 tcacggttgg tgaaacgctt taccctactg aatcaggccg ttgtgcaaat tggtctggga
     4201 ggcacggaaa cggctattcc tgtttatggg aatgatgaac tggggagaat tgcaggatta
     4261 ttacgccata ctctcgggca gctcaatgtg caaaaacagc aacttgaaca agaaattacc
     4321 gatcgaaagg tgatagaagc cgatctgcga gccacccagg acgaactgat tcagacagcg
     4381 aagctggcgg tagtcgggca aacgatgacc acgctggccc acgagatcaa tcagccgcta
     4441 aatgcgctgt cgatgtatct gtttacagcc cgcagggcca ttgaacagac ccagacagaa
     4501 caggccagca tgatgcttgg taaagccgaa ggggtgattg gtcgcattga cgccattatc
     4561 cgttcactac ggcagtttac ccggcgcgcc gaactggaaa tatcactcca ttccgttgat
     4621 ttagcacaga tgttcagtgc ggcctgggaa cttctggcca tgcgtcatcg ccctttgcaa
     4681 gctacgcttg ttctgccgca aggtacagcc acagtttcag gcgatgaggt cagaacccag
     4741 caggtactgg ttaacgtact ggcgaatgcg cttgatgttt gtgggcaagg cgctgtcatt
     4801 accgttaact ggcaaatgca gggtaaaacg ctgaacgtat tcattggcga taatggcccg
     4861 ggctggcctg aggcattgtt gccttcgtta ttgaagccgt ttaccaccag taaagaagta
     4921 ggactgggta ttggtctttc aatttgtgtg tcgttgatgg agcaaatgaa aggtgaatta
     4981 cggctggcat caacgatgac caggaatgcc tgtgtggtac tgcaattcag actaacggat
     5041 gtggaagatg ctaagtagtg aatattcgat tctgctgatt gatgatgatg ctgacgtgct
     5101 ggacgcctac acacagttac ttgaacagtc aggctatcgg gtttttgcct gtaacaatcc
     5161 ttttgaggcg cagacgtgga tacaaccaga ctggccaggc attgtgctaa gtgatgtgtg
     5221 tatgcctggc tgctctggta tcgatttgat gatgttattt caccaggatg atcaacagat
     5281 ccccattctg cttattaccg ggcacggcga cgtgccaatg gcagttgatg cggtaaaaaa
     5341 aggcgcgtgg gattttttgc aaaaaccggt tgatcccggc aaattgcttt ctctggttga
     5401 agaggcgcta cgccagcgtc aatcaatcat tgcgcgtcgt cagtattgtc aacagacgtt
     5461 gcaggtggag ttgataggga gaagtgaatg gataaatcag tatcggcgac gtttacagca
     5521 gttgtctgaa acagatattg ccgtctggct ctacggtgcc ccaggcacag gacggatgac
     5581 cggtgcacgt tatctgcacc aatttggacg taatgcacaa ggcgaattta tctaccgcga
     5641 gctgacacca gacaatgcac cacagcttaa tgatttcatt gcgctggctc aggggggaac
     5701 attagtgctc agccatcctg aacacctgac gcgtgaacag cagtaccatc tggttcagtt
     5761 acaaagccag gaacaccgac cttttcgcct gattggtatt ggagacactt cgctggtcga
     5821 gctggccgcc agcaaccata tcatcgcgga gctctattac tgctttgcaa tgacacaaat
     5881 cgcttgcctg ccattaactc aacgccccga tgatattgaa ccactgtttc gccattacct
     5941 ttgtaaagca tgtcaacggc ttaaccatcc tgttccggaa gtgggaaaag agatgctcaa
     6001 agagatgatg cgccgcatgt ggccaaataa tgtgcgagag ctggccaatg cagcagaact
     6061 atttactgta ggagtattac cgctggcaga aacagcaaat ccgttgatgc acgtcggaac
     6121 cccgacaccg cttgacagac gagtggagga cgctgagcga cagataatca cagaggcact
     6181 taatatccat caggggcgta tcaatgaggt tgctgaatat ttacaaatcc cacggaaaaa
     6241 actttatctc agaatgaaaa aatatggtct tagtaaggag cattataaaa tttaaccatc
     6301 agacttgtga gcttgttcat aatgttatgt cattgcctga tgttgataaa aagacggacg
     6361 ctccccctgt aaaacagggg gctataacgt acctggaggc ataaacccat gctgttcaag
     6421 tatacctctg gcaacaggtg aaactaaaaa gtcggcaagt ggcagggcct tatcggtcag
     6481 acaggcaaat ccatagatag caatcggatt ataaggttcc ggtatttcta taacttttac
     6541 tgaatcaatt gattgcaagc caggaacata attggcataa ccgatgaaca gatcagtata
     6601 atcattttca attaaccact gcgctgctat cgcatttcct ggaagaggca acgaatgcct
     6661 ccccccaact aatgctacag cccgttgtct tattttttca ccgacactgc ccatattttc
     6721 aaacagttcc tgagtgtaat caccagatgg atcacatccc gccgttgatg ttccgattcg
     6781 caatgtttcc cgatttaata aagaatacca gtcgtcattc tcattgaatt tattcgcccg
     6841 aacatacaaa cacaaacgat tgctggcaaa aggtttaata cacaatgctc gtcctgcgga
     6901 cattaatatc tgtgggtgag tcatattggc tgatgcaaaa aaatcgcatg cctcacccgc
     6961 ctcaatacgc tcccttaata tacccgctgg tccaaaatca cactgtatat tatcggcctg
     7021 atactctgac acaagtgatt tccatacccg gcgtaaactg cccgcagcaa gcactcgcat
     7081 tagtcttcac cctgataatc agtacggtaa aacgttctat accagccatt gactatttta
     7141 tgcatatcaa catcattgaa tttttctgga tacagctttt tcgccatcca caattcccca
     7201 atccccatag cctcgggcat cggatagccc catgctttgg catactctgg catcaaataa
     7261 acacaatgat ttttgacagc atctattacc tgccactgtg ggcttgactg tatttcattc
     7321 actactgcag gataacgatt ctgcacaaaa attacctgag gattccattc aatgacttgc
     7381 tctatcgaga cctgtttgaa acctttaatg gtggaagcgg cgacgtttac tgcgccagca
     7441 tgtttcatca ttaatcctgt atattttccg gaaccataag tggtcaattc aggattcgcc
     7501 atataggcac gaacacgctg ctcttcgggt agaagttgca ggcggttact caccattctg
     7561 cggccatttt cagttgcctt gattaacgct ttagcttctt caggcttatt gattatatta
     7621 ccaatcagca taatcccttc atataaccct tttacgtaag cctgttcttc atctgccatg
     7681 actgggttaa gtttatttct ctccccctca tcgtcatgcc gcaaagaaat cgctactgcc
     7741 ggaattccca gactactgat tttatctatc atttcctgcg gagcataatt ggtgacaaaa
     7801 acgacttgcg gatgcaaagc caccaatttt tcggcatcaa catgggttaa atcgcctaat
     7861 gacgccttgt tgctcagttc tggcgcaaga cgtgcataat tactgccaag ctgttgtttc
     7921 cagtttgaca taacaccaac gattttatca gttgcattca tctgcactaa aagattcaac
     7981 gtttgatgct ggagcaccac gatacgatca actttgtcgg gtactttcac ctcccgacca
     8041 atctgatcgg tgaaagttcg ttcagcttgc gtagtaaatg ataaagaaag caaaaaaatc
     8101 agtaataaaa acaacttatt atttattttc atattccatc acactacaaa tcggtatata
     8161 aataactata tatcgaaatg gtagcaccag tcaggctgtg ggaaaagcga ttccggtata
     8221 tttttgatgt gaccgtatgc agagaatgtg acgtttatgt gttcagcaga ggttttcaat
     8281 tccgttatct gtaaccctga atacgcatga tcataatagc aaagacaaag gagtcgttac
     8341 gttgccgcac cctatccatt caccatcgcc tgagacgata aaatatcagt accatcagct
     8401 tgtacataaa cggattatac tgatttcaat aataacgctg gcgatgctct cctcactatt
     8461 tctggacatc atactgggct cttccaattt gccagtcagt gagctatggc atgctttagc
     8521 tcatccgttt agtcgtgata ccagcgtcca tgtcattgtc tgggaaatac gattacccta
     8581 cgccttaatg gctttattgg ttggcatgac gctaggactc gcgggggcag aaatgcaaac
     8641 tatcctcaac aatccattgg caagtccctt tacacttgga gtctcttctg cggcagcatt
     8701 tggtgcagct ctggcgattg tattaggaat tggtattcct ggtatatcgg cagcatggtt
     8761 tgttcctgca aacgcgttta tatttgctct tctgtcatcg ttattacttg atagcatgtc
     8821 tcgttggcta agaatagcaa gctcaggcat gattctgctg ggtatagcat tggtattcac
     8881 gtttaatgct cttgtggcaa taatgcagtt tattgcggat gaggatacac tccaggggct
     8941 ggtattctgg acaatgggaa gcctggctcg tgcatcctgg aacagactgg gtattttatc
     9001 aggcgtattt atcattgttt ttttatggtc attttcccgc tcatggcaac ttacggcatt
     9061 acgcatggga gaagaaagag cgttaagttt tggtatcaat gcacagcaac tgcgtctttc
     9121 ctccctgtta cgtataagtt tgcttgcagc attatcggta gcctttgttg ggccgattgg
     9181 ttttattggt cttgtctctc cacatatttc ccggatgcta tttggtgagg aacatcgttt
     9241 ctatctaaca ggaagtgtat taaccggagg tctggttctt tcactggctt caattgcctc
     9301 gaaaaacatt atttcgggtg tcattattcc aataggcatc gtcacctcgc ttgtgggtgt
     9361 tccattattt attagtatta ttctgcgcta tcgcaggagt ttgttatgaa aggtctttca
     9421 ctgcataaat taaatgcagg ttatcagaaa aaaaacgtaa tatgtgatct cagtttacct
     9481 gaactgcaac gaggaaaaat aacaatcatt ctgggaccaa atggttgcgg taaatcgaca
     9541 ttgttacgtg cactggcggg actgaataag gccaacggta aggtatttct tgatggtgtc
     9601 aacctaatgc aaatgccatt ttctgcgcgt gccgacagta caatattttt gccacagttt
     9661 ttaccacatg atgtgcatct gaatgtgttg gaatctattc tggtttccct acgcgcgtca
     9721 aagatgggcg ggaaacatga aaaaacacaa tcaatagcta ttctcaaaca actggggata
     9781 gaacatctgg ccttttgtta tctggatcaa ctatccggag gacaaagaca actcgtcggt
     9841 ttggcacaat cattggtccg taatccatcg atattactac tggatgagcc actaagtgca
     9901 ttagatttaa accatcagtt ccatgttatg gatttagtaa aaagtgagac acgtaatcgc
     9961 aatatagtca ccattatggt agttcacgac ttaaatattg cactacgata tggtgattat
    10021 gtcgttatgt taaaaaaagg caaacttatt gccagtggaa aacccaaaga ggtcattacg
    10081 ccagagaatc tggctaatgt ttatcgcatc cgtggacgaa tagagcattg ttctcatggc
    10141 ttgttacaac ttatcgttga tggtgtcatt ccggattaac aaaatggatg gaagatttct
    10201 ctcccggaac ttctcatcac tttttgacac catcatcaac gatgattaac taaaacagtg
    10261 ttccctttga tgatgagttt gtttatatgc agcatgagga ttagctcgtg ctttatatca
    10321 gaactcacct catataaaaa cgagtaattt tcaacttttc aaggtccaat gcaaggtcaa
    10381 tttacaatga atgccctgtt ggcctaaaat acacccatca aatactattt ttgatcactt
    10441 gtataacggt ttcgatacgc tcttttattt cctctagttg ctgctgacga ccttcaggtg
    10501 ttaatgtctg atcttcgttc agatccaaaa cccatcgacg tggaatcaat gcaccaaaaa
    10561 caatatccat taacatcccc acacaattcc ccgtattttt aacagtaatg cgtccttgag
    10621 cttgctgaat atccaaccac tcaattaaca gttctcttga gcgaagtgtt ttagttgtat
    10681 ataaatagtc tgaaagatct ggaaagagaa tagactcacg cgtcattaat tttaataacg
    10741 cttctctctc tttagcgtct tcctgactta tatctaatcg gaatatgcga aacagtgctt
    10801 cattaagagg gagctcttca ttttccggtc tcggaagatc gagaaacagt gctttatatt
    10861 ccttaataac ttcagagaaa atttcttttt tattgctgaa cacttcataa atcgttcttt
    10921 ttgatacttt tgcccggcgg gcaatttctg tggttgaggt ttgagcaaaa ccaagctcaa
    10981 tgaaggcact gtacgcccca ataactattg cttttttacg ctcactggcc atcgatggtg
    11041 tgttctggcg cacaccggat acatcagaga acgatgcagc cttcccccgc cagacacatg
    11101 ccgggaaccc ggcgctctac ccgcaggtca aaatggtctg ccagatggaa ctgaccagcc
    11161 atctgctgac ggctgcagcc ttcggcacga tgaagaacag cgaaaatgag cttgctgagc
    11221 aacttataga acaaaccggc gataa
//
LOCUS       DADTFG010000150        10927 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.151, whole genome shotgun sequence.
ACCESSION   DADTFG010000150 DADTFG010000000
VERSION     DADTFG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.151"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            93..1235
                     /locus_tag="J1138_003835"
     CDS             93..1235
                     /locus_tag="J1138_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA8455575.1"
                     /translation="MECDLLMIKIEKVINKKDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTELTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1242..2006)
                     /gene="glcC"
                     /locus_tag="J1138_003836"
     CDS             complement(1242..2006)
                     /gene="glcC"
                     /locus_tag="J1138_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBA8455576.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2257..3756
                     /gene="glcD"
                     /locus_tag="J1138_003837"
     CDS             2257..3756
                     /gene="glcD"
                     /locus_tag="J1138_003837"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBA8455577.1"
                     /translation="MSILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQMLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3756..4808
                     /gene="glcE"
                     /locus_tag="J1138_003838"
     CDS             3756..4808
                     /gene="glcE"
                     /locus_tag="J1138_003838"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBA8455578.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4819..6042
                     /gene="glcF"
                     /locus_tag="J1138_003839"
     CDS             4819..6042
                     /gene="glcF"
                     /locus_tag="J1138_003839"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBA8455579.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGMVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6047..6451
                     /locus_tag="J1138_003840"
     CDS             6047..6451
                     /locus_tag="J1138_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBA8455580.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6473..8644
                     /gene="glcB"
                     /locus_tag="J1138_003841"
     CDS             6473..8644
                     /gene="glcB"
                     /locus_tag="J1138_003841"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBA8455581.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDHVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGNTAALTCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFSRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            9000..10682
                     /gene="glcA"
                     /locus_tag="J1138_003842"
     CDS             9000..10682
                     /gene="glcA"
                     /locus_tag="J1138_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBA8455582.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2540 a         2724 c         3069 g         2594 t
ORIGIN      
        1 tttgtttgta ttcattgggt tgattattaa gatgccgttc gttgctgttg caacttgttt
       61 ttcaccgtta attatttgtt atccacagag atatggaatg cgatctgctg atgattaaaa
      121 ttgaaaaagt aattaataaa aaagacctta aggcatttat cgcttttccg tcatcacttt
      181 atcccgatga tccaaactgg ataccccctt tattcattga acgcagcgaa catttgtctg
      241 cgaagaatcc ggggacggac catatcatct ggcaggcgtg ggtggcaaaa aaagcggggc
      301 agatagttgg gcgtattacc gcgcaaatag ataccttgca ccgtgagcgt tacggtgaag
      361 ataccggtca tttcggaatg attgatgcca ttgatgatcc gcaggttttt gccgcgttgt
      421 ttggcgcagc ggaggcgtgg ttgaagtcac aaggtgcaag taagatcagc gggcctttca
      481 gcctgaatat caatcaggaa agcggattac tgattgaagg ttttgacaca cctccctgtg
      541 cgatgatgcc acacggcaaa ccgtggtatg ccgcgcatat tgaacaactg ggttatcaca
      601 aaggcattga tttactggcg tggtggatgc agcgaaccga gctcactttc tctccggcgc
      661 taaaaaaact gatggatcag gtgcgcaaaa aagtaaccat tcgctgcatc aatcgccagc
      721 ggtttgccga agagatgcag atcctgcgtg agattttcaa ttctggctgg cagcacaact
      781 ggggatttgt gccgtttacc gaacatgaat tcgcgaccat gggcgatcaa cttaaatatc
      841 tggtgccgga cgatatgatc tacatcgctg agattgattc tgcaccctgc gcgtttattg
      901 tcggcttgcc gaacatcaac gaggcgattg ccgatctgaa cggatcgctc tttcccttcg
      961 gctgggcaaa attgctgtgg cgtttgaaag tcagcggtgt gcgaactgcg cgagtgccgc
     1021 tgatgggcgt gcgggacgag tatcagttca gccgcatcgg cccggtgatt gcgctgttat
     1081 tgattgaagc cttacgcgat ccgtttgccc gccggaagat cgatgcgctg gaaatgtcat
     1141 ggatcctcga aaccaataca ggcatgaata acatgctgga acgcattggg gcggaaccgt
     1201 acaagcgtta ccgattgtat gaaaagcaga tttgatggca gtcacccctg cttcatctcc
     1261 agcggcaccg ccgagcgaat caaatggtgg ccttccagct cgatatcgtg gagattcttt
     1321 ttcacggtcc gcacatgatc gcgtgctgcg cgctgggcaa cgtgcggcag ccgctgcaac
     1381 accgcgttgt agatccgcgc atgctggcga tcgatctgct ttttttgctg tggtcgatgg
     1441 tagagattat ttacgctggc aaacaccgaa ttaaacatca ggtcggtcaa tgattgcagc
     1501 gtaaacacca gcacctgatt gtgagaagcc tgacaaatgg cgagatggaa agcgtgatcc
     1561 aactgcgcat gttcgatcag cgaaatctct ttgttgttct cactggcggc gagcattttt
     1621 tcataacagc gggttatcac aacaaaatca gcctgggttc ccagcgttgc cgccagcctt
     1681 gccgattcgc cctccagtaa tgcgcgaacg tcgagcagat cgtacagcgt tcgcggctgc
     1741 gtactgaaca gatggatcag cgggctggcg tcctgcaccc gattaagccg tgcgacacga
     1801 gaatcgcgac cctgcgccgt ttcaataatc ccgcgcccgc gcagcacggt cagcccttca
     1861 cgcagtgcgg agcgtgagaa gccgagcttt tcacacagtc gacgttccga gggaagcggc
     1921 tgaccgacct tcaacacgcc gtcgataatt aaccgttcga tactctctgc aaccacttcg
     1981 caaatagggc gacgttcatc tttcattccc ggacctcgtg cacaggtagg accaattttt
     2041 ctgttcttaa ctcgcaaaac acgcacatca cgtaagtgat tatgttacat caatttaaca
     2101 ttaagttaac caaagcaagg tcacagagct ggaaaaaaaa tggtctgacc ggtagctaaa
     2161 gagatagacg aaaacgaaaa gcccgcttaa taactgttca cagaagcagc gcgaaaaaat
     2221 cagctgccac acaacacaac aaagcgaacc ctactcatga gcatcttgta cgaagagcgt
     2281 cttgatggcg ctttacccga agtcgaccgc acatcggtac tgatggcact gcgtgagcat
     2341 gtccctggac tggagatcct gcataccgat gaggagatca ttccttacga gtgtgacggg
     2401 ttgagcgcgt atcgcacgcg tccattactg gttgttctgc ctaagcagat ggaacaggtg
     2461 acggcgattc tggctgtctg ccaccgcctg cgtgtgccgg tggtgacccg tggcgcaggc
     2521 accgggcttt ctggtggcgc gctgccgctg gaaaaaggtg tgttgttggt gatggcgcgc
     2581 tttaaagaga tcctcgacat taaccccatc ggtcgccgcg cgcgcgtgca gccgggcgtg
     2641 cgtaacctgg cgatctccca ggccgttgcg ccgcataatc tctattacgc gccggatccg
     2701 tcctcacaaa tcgcctgttc gattggcggc aatgtggcgg aaaatgccgg tggcgttcac
     2761 tgcctgaaat atggtctaac cgtacataac ctgctgaaaa ttgaagtgca aatgctggat
     2821 ggcgaggcgc tgacgctggg atcggacgcg ctggattcgc ctggttttga cctgctggcg
     2881 ctgtttaccg gatcggaagg tatgctcggc gtgaccaccg aagtaacggt gaagctgttg
     2941 ccgaagccgc ccgtggcgcg ggttctgtta gccagctttg actcggtaga aaaagccgga
     3001 cttgcggttg gtgacatcat cgccaatggc attatccccg gtgggctgga gatgatggat
     3061 aacctgtcga ttcgcgcggc ggaagatttt attcatgccg gttatcccgt cgatgccgaa
     3121 gcgattttgt tatgcgaact ggacggcgtg gagtctgacg tacaggaaga ctgcgagcag
     3181 gttaacgaca tcttgttgaa cgcgggtgcg actgacgtcc gtctggcaca ggacgaagca
     3241 gagcgagtac gtttctgggc cggtcgcaaa aatgcgttcc cggcggtagg acgtatctcc
     3301 ccggattact actgcatgga tggcaccatc ccgcgccgcg ccctgccagg cgtactggaa
     3361 ggcattgccc gtttatcgca gcaatatgat ttacgcgttg ccaacgtctt tcatgccgga
     3421 gacggcaaca tgcacccgtt aatccttttc gatgccaacg aacccggtga atttgcccgc
     3481 gcggaagaac tgggcgggaa gatcctcgaa ctctgcgttg aagttggcgg cagcatcagt
     3541 ggggaacatg gcatcgggcg cgaaaaaatc aatcaaatgt gcgcccagtt caacagcgat
     3601 gaaatcacga ccttccatgc ggtcaaggcg gcgtttgacc ccgatggttt gctgaaccca
     3661 ggaaaaaaca ttcccacgct acaccgctgt gctgaatttg gtgccatgca tgtgcatcac
     3721 ggtcatttac cttttcctga actggagcgt ttctgatgct acgcgagtgt gattacagcc
     3781 aggcgctgct ggagcaggtg aatcaggcga ttagcgataa aacgccgctg gtgattcagg
     3841 gcagcaatag taaagccttt ttaggtcgcc ctgtcaccgg gcaaacgctg gatgtgcgtt
     3901 gtcatcgcgg cattgttaat tacgacccga ccgagctggt gataaccgcg cgtgccggaa
     3961 cgccgctggt ggcgattgaa gcagcgctgg agagcgccgg gcaaatgctc ccctgcgagc
     4021 cgccgcatta tggtgaagaa gccacctggg gcgggatggt cgcttgcggg ctggcggggc
     4081 cgcgtcgccc gtggagtggt tcggtccgcg attttgtcct cggcacgcgc attattaccg
     4141 gcactggaaa acatctgcgt tttggtggcg aagtgatgaa aaacgttgcc ggatacgatc
     4201 tctcacggtt aatggccgga agctacggtt gtcttggcgt gctcactgaa atctcgatga
     4261 aagtgttacc gcgaccgcgc gcctccctga gcctgcgtcg ggaaatcagc ctgcaagaag
     4321 ccatgaatga aatcgcccag tggcaactcc agccgctacc cattagtggc ttatgttact
     4381 tcgacaatgc gttgtggatc cgccttgagg gcggcgaagg atcggtaaaa gcagcgcgtg
     4441 aactgctggg tggcgaagag gttgcaggtc agttctggca gcaattgcgt gaacaacaac
     4501 tgccgttctt ctcgttacca ggtaccttat ggcgcatttc attacccagt gatgcgccga
     4561 tgatggattt acccggcgag caactgatcg actggggcgg ggcgttacgc tggctgaaat
     4621 cgacagccga tgataatcaa atccatcgca tcgcccgcaa cgctggcggt catgcgaccc
     4681 ggtttagtgc cggagacggt ggctttgccc cgctaccggc acctttattc cgctatcacc
     4741 agcagcttaa acagcagctc gatccttgcg gcgtgtttaa ccccggtcgc atgtacgcgg
     4801 aactttgagg agcaggctat gcaaacccga ttaacagaag agatgcggca gaacgcgcgc
     4861 gcgctggaag ccgacagcat cctgcgcgcc tgtgttcact gcggattttg taccgcaacc
     4921 tgcccaacct atcagcttct gggcgatgaa ctggacgggc cgcgcgggcg catctatctg
     4981 attaaacagg tgctggaagg caacgaagtc acgcttaaaa cacaggagca tctcgatcgc
     5041 tgtctcactt gccgcaattg tgaaaccacc tgtccttctg gtgtgcgtta tcacaatttg
     5101 ctggatatcg ggcgtgatat tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg
     5161 ctgcgcgaag gattgcgcca ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag
     5221 gtagggatgg tgctgcgacc gtttttaccg gaacaggtca gagcaaaact gcctgctgaa
     5281 accgtgaaag ctaaaccgcg tccaccactg cgccataagc gtcgggtttt aatgctggaa
     5341 ggctgcgccc agcctacgct ttcgcccaac accaacgcgg caactgcgcg agtgctggat
     5401 cgtctgggga tcagcgtcat gccagctaac gaagcaggct gttgtggcgc ggtggactat
     5461 catcttaatg cgcaagagaa agggttagcg cgggcgcgca ataatattga tgcctggtgg
     5521 cccgcgattg aagcaggtgc cgaggcaatt ttgcaaaccg ccagcggttg tggcgcgttt
     5581 gtcaaagagt atgggcagat gctgaaaaac gatgcgttgt atgccgataa agcgcgtcag
     5641 gtcagtgagc tggcggtcga tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg
     5701 gcaattcgcg gcgataaaaa actggccttc cactgcccgt gtaccctaca acatgcgcaa
     5761 aagctgaacg gcgaagtgga aaaagtgttg cttcgtcttg gatttacctt aacggacgtt
     5821 cccgacagcc atctgtgctg cggttcagcg ggaacatatg cgttaacgca tcccgatctg
     5881 gcacgccagc tgcgggataa caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc
     5941 gtcaccgcca acattggttg ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt
     6001 cactggattg aaattgtaga acaagccctt gaaaaggaat aacaaaatga aaactaaagt
     6061 cattcttagc cagcaaatgg cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa
     6121 aaataactgg tctgtttcca ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag
     6181 tcgcatggac gattgcgcgc cgattgcggc ttacatctcc caggagaaag cgcgtaccgc
     6241 cgcgctgggg cgtcgtgaaa ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc
     6301 gttcgtgact gcgccgttac tgacgtcgct ggaaggtggc gtaccggttg ttgtggatgg
     6361 gcaaattatt ggtgccgtgg gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc
     6421 gaaagcggca gcagcggtgt tggcgaaata agcgaaaacg aggagataaa caatgagtca
     6481 aaccataacc cagggccgtt tacgcattga cgccaatttt aaacgttttg tggatgaaga
     6541 agttttgcct ggcgtggaac tggatgctgc cgcgttctgg cacaatgttg atgagatcgt
     6601 tcacgatctg gcgccagaga atcgtcagtt gctggcagag cgcgatcgca ttcaggcggc
     6661 gcttgatgag tggcatcgca gcaatccggg gccggtaaaa gataaagcgg cctataaatc
     6721 tttcctgcgt gaactgggct acctggtgcc acagcctgac cacgttacgg tggaaaccac
     6781 gggcatcgac agcgaaatca ccagtcaggc ggggccacag ctggtggttc cggcgatgaa
     6841 cgcccgctac gcgctgaacg cggcgaacgc tcgctggggc tcactgtacg atgcgttata
     6901 cggcagcgac atcatcccgc aggaaggggc gatggtcagc ggctacgatc cgcagcgtgg
     6961 tgagcaggtt atcgcctggg ttcggcgttt cctcgatgaa tcactaccgc tggaaaacgg
     7021 cagctatcag gatgtggtgg cgtttaaggt ggtcgataaa caattacgca tccagttgaa
     7081 aaatggtaaa gaaaccacgt tacgtactcc agcacagttt gtcggttacc gtggcaatac
     7141 tgctgcgctg acctgcattt tgctgaaaaa taacggcctg catattgagc tacaaatcga
     7201 tgccaacggg cggattggca aagacgattc ggcgcacatc aacgatgtta tcgtcgaagc
     7261 ggccatcagt accattctcg actgcgaaga ttcggttgcg gcggttgatg cggaagataa
     7321 aatcctgctg taccgcaacc tgctgggcct gatgcagggg actctgcaag agaaaatgga
     7381 gaagaacggt cggcaaatcg tgcgtaaact gaatgacgat cgtcagtaca ctgccgccga
     7441 tggatctgaa atttctctgc acggacgctc gttgctgttt atccgcaacg tgggtcattt
     7501 gatgaccatt cctgtgattt gggacagcga aggcaatgaa atcccggaag gcattcttga
     7561 tggcgtcatg actggcgcga ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac
     7621 tggcagcgtc tatattgtga aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa
     7681 caaactgttt tcccgcgttg agacaatgct cggtatggca ccgaataccc tgaaaatggg
     7741 cattatggat gaagaacgcc ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg
     7801 caaccgcgtg gcgttcatca ataccggttt cctcgaccgt accggcgatg aaatgcattc
     7861 ggtgatggaa gcgggcccga tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa
     7921 agcctacgaa cgtaataacg tgctttccgg tctgttctgt gggctacgtg gaaaagcgca
     7981 aatcggtaaa ggcatgtggg caatgccgga cctgatggca gacatgtaca gccagaaggg
     8041 cgaccaattg cgtgccgggg caaacacggc ctgggttccg tcgccaactg ccgctacgct
     8101 ccatgcgctg cactaccacc aaaccaacgt acagagcgtg caagccaaca ttgcccagac
     8161 cgagttcaat gctgaatttg aaccgctgct ggacgatctg ctgacgattc cggttgctga
     8221 aaacgctaac tggtcggcgc aagagatcca acaagagctg gataacaacg tgcaggggat
     8281 tctggggtac gtggtgcgtt gggtggagca ggggattggt tgttcaaaag tgccggatat
     8341 tcacaacgtg gcgttgatgg aagaccgcgc aacgctgcgt atctccagcc agcatatcgc
     8401 caactggtta cgtcacggta ttctgaccaa agatcaggtg caggcgtcgc tggagaatat
     8461 ggcgaaagtg gttgatcagc aaaacgctgg cgatccggct tatcgtccaa tggtggagaa
     8521 tttcgctaac tcgtgtgctt ttaaagctgc ctgcgatttg atcttcctcg gcgtgaaaca
     8581 gccaaacggc tataccgaac cgttattaca cgcatggcgt ttacgcgaaa aagaaaatca
     8641 ttaatagcgt caaaaaaagc cccggcaaga tttattgtcg gggctatgat agtaactgcg
     8701 gtagtacatt tatcccggaa ataaccggag cgattatcag gggttaacaa tattaaatac
     8761 ccgtagcgca tcactagcgg gattaaaaca acaatatgga catcttaacg atactctgcg
     8821 cctgcgtgtg cggaacagaa acccttaccc gatatacttg ttatgccaga ggtagcaatt
     8881 gcgttagcgg cgtactgttg ctgtaaagta taacgcctat attttacagt tcagtcctct
     8941 tttgcttatt acaaccttaa gtatatcaag catataaaga taataagaga ctgaacaata
     9001 tggttacctg gacccaaatg tatatgccga tgggaggact ggggctatcc gctctggtcg
     9061 ccctgatccc aataatattc ttcttcgttg cactcgcggt attacgtctg aaaggacatg
     9121 tcgctggagc aataaccctt atattatcta ttctgattgc gatattcgcc tttaaaatgc
     9181 cgattgatat ggcatttgct gctgcgggct atggctttat ttatggttta tggcctattg
     9241 cgtggattat tgtcgcggcg gtattcctgt ataaattaac cgttgccagc ggccagttcg
     9301 atattatccg cagctcggtt atctccatca ccgacgatca gcgtttgcag gtgttactga
     9361 ttggtttctc ctttggtgcg ctgctggaag gggcggctgg ctttggcgca ccggtggcga
     9421 ttaccggtgc tctactggtg ggcctgggct tcaaaccgct atacgcggcg gggctgtgtc
     9481 tgattgccaa cactgcgccg gtggcgtttg gtgcgttggg tgtgccaatt ctggtcgccg
     9541 gtcaggtaac gggaatcgat ccgttcgaca ttggcgcaat ggcgggacgt cagttaccgt
     9601 tcctgtcggt tcttgtgccg ttctggctgg tagcaatgat ggacggctgg aaaggggtga
     9661 aagagacgtg gccagcggcg ctggttgcag ggggaagctt cgctgtcact cagttcttta
     9721 cctctaacta tattggtccg gaactgccgg atattacttc ggcgctggtg agtatcgtct
     9781 cactttcttt atttcttaaa gtctggcggc caaaaaatac cgaaacggca atcagtatgg
     9841 gacaatccgc aggtgcgatg gtggtgaata aaccgtcttc tggcggtccc gtgccttcag
     9901 aatatcgtct ggggcaaatc attcgtgcgt ggtcaccgtt tttaatctta acggtgttgg
     9961 tcaccatctg gaccatgaag ccgtttaaag cgttatttgc tccgggcggc gcgttttatt
    10021 cgctggtgat taatttccag atccctcatt tgcatcaaca agtgttgaaa gcggccccca
    10081 ttgtcgccca gccgacgcca atggacgcgg tgtttaaatt cgaccccctc tctgctggtg
    10141 gcaccgctat ttttattgcg gcgattatct cgatcttcat cctcggcgtg gggatcaaga
    10201 aaggtattgg tgtctttgcc gaaacgctaa ttagtctgaa gtggccgata ctgtcgattg
    10261 gcatggtgct ggcatttgcc ttcgtgacca actattccgg catgtccacc acgctggcgc
    10321 tggtactggc tggtacaggc gtgatgttcc cgttcttctc accgttcctc ggctggctgg
    10381 gcgtattcct taccggctct gacacttctt caaacgccct gtttggttca ctgcaatcga
    10441 ccacggcgca gcaaatcaac gtctctgaca ccctgctggt ggcagcaaac accagcggcg
    10501 gcgtaactgg caagatgatc tccccgcaat ctatcgccgt ggcctgcgcc gcgacgggca
    10561 tggtgggtcg agaatctgaa ctgttccgct acaccgtgaa gcacagtctg atttttgcca
    10621 gcgttatcgg cattatcacg ctgttgcagg cgtatgtgtt taccgggatg ttagtttcgt
    10681 aagtcaataa agccggatga tcgttaatat catccggctt cgtttacatt attctgtctt
    10741 attaatggaa aattatttcc tgctttatta tccatgcggc aataatgttt tgcgttcaga
    10801 taacatgcta aataattaag agcgtgagaa catctatttt ttatcacgca aaacaagtct
    10861 gtattaatta tgttcgcttg atgagcaaac tccgttctat ctgttgcttt tgcaatctta
    10921 atcgtta
//
LOCUS       DADTFG010000151        10864 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.152, whole genome shotgun sequence.
ACCESSION   DADTFG010000151 DADTFG010000000
VERSION     DADTFG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.152"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            252..1007
                     /locus_tag="J1138_003843"
     CDS             252..1007
                     /locus_tag="J1138_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8455583.1"
                     /translation="MYLVDERRKLILEDLMSAGTVYVSDLARKYEVTYETIRKDLTHL
                     EQKGLLIKSHGGATLKQRAIEHPFQVREKENNSYKKAIARKALDFIPPNSSMIIGTGS
                     TTFELANLLCMKSGFKIFTDSLPVASVLIESDNQVFLFGGELRRKSSSVFGGWAVSMI
                     NDIHVDLCFIGTDGFSNMTGPSTPSSSDAFLDRTIISHSDKKYILGDYTKFERSSLFK
                     ICDWSEITALITNDHADKEGIAEVAKYTSVITC"
     gene            1346..1789
                     /locus_tag="J1138_003844"
     CDS             1346..1789
                     /locus_tag="J1138_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HBA8455584.1"
                     /translation="MISISVADGAGLQNYDAVLEHIYNTHRETNTVKESWLKAMRKRE
                     AEYPTGIELEGYSIAIPHCDSEHASQPAIYIIRLPEAIPVNRADEDGILWVRLVVNLI
                     VTDPADQLRLLKSLFNHLQNEEFYQNLLTLPVDDAKALFVSKIIQ"
     gene            1808..2101
                     /locus_tag="J1138_003845"
     CDS             1808..2101
                     /locus_tag="J1138_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA8455585.1"
                     /translation="MINILVACGSGVATSTLAADEVKSVCAEYGITAFKINKCSMAEL
                     SSEMAHADIVLTTNNYKGDIGKPHMSVADFVTGINEGALRKKLGELLTELANS"
     gene            2122..3375
                     /locus_tag="J1138_003846"
     CDS             2122..3375
                     /locus_tag="J1138_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA8455586.1"
                     /translation="MEMLKNLMETITGMGATAILPLVIFILGLVFRMKPGAAIKSGIT
                     VGIGFIGLGLVVGLLNSSLQPAIEYYSKVGSGFTVADIGWPAVGAAAWVAPFAALVIP
                     VGIVLNLILVRLKLTKTLNVDIWNYMHFLVPGALAYFVFDSFIIGFSVAVALSIAALF
                     IGDLIAPRWQKYYGLEGTTCTTMIHIGWTLPFAWVVNKIIDYIPGLNKLDVDLNSVQK
                     RLGVFGEPAIIGIIVGALLGVLTKQAITTIVPMAMGVAGVMVLLPKVVGVLMEGLNPI
                     GKSAKEIMQKQMGKDAELNIGMDCALALGDPATVTVTVITIPLTMLCALVLPDIKIFP
                     IGVLMSIIYMTTMTVMVSNGNVVRSIISTLLFCVVVMYLGGYVAPGATQFLAGAGVGL
                     QGQGTDFVLTGPWEILTYWLSTVLH"
     gene            3398..4042
                     /gene="rpe"
                     /locus_tag="J1138_003847"
     CDS             3398..4042
                     /gene="rpe"
                     /locus_tag="J1138_003847"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA8455587.1"
                     /translation="MAKILPSIFGADILRLKDEIHFLEEEQTEILHVDLMDGTYVSNI
                     AFGPNQIAAMKKASSMIFDVHMMLANPERHIDDVINTGAEMISVHYESTPHVHYIIQK
                     IKKAGRKAGVVLNPGTPGSVIEYLLDDIDYILLMTINPGQPGQTFIHKSLEKIRNTKK
                     MISGRDIQIEVDGGVNLDNVKQVKEAGADLIVVGGALFNKQPHESYQALKIAVQ"
     gene            4138..4890
                     /locus_tag="J1138_003848"
     CDS             4138..4890
                     /locus_tag="J1138_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA8455588.1"
                     /translation="MSNFAGKVVLVTGGAHGIGKGIAKQFADRGAHTVIVDYNEENGK
                     ATAAELTFGDVKSLFVKTDVSDPQALVDVREKVIATFGRIDVLILNAGVAFANKVNNI
                     SFEEWNKTIAINLSGLFNTVKAFYDDFLTNKGSIVYISSGSALSGTGGGVSYPASKAG
                     GEGLIRGLAKELGPKGVNVNAIAPRLIDTGEMMRVNYPTQADLDAVLEKIPVRRLGTI
                     DDVANLAIFLADKDNSYIQGQTILMDGGRTIA"
     gene            4906..5466
                     /locus_tag="J1138_003849"
     CDS             4906..5466
                     /locus_tag="J1138_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA8455589.1"
                     /translation="MSVQENTINILHELENNALKIDDSQAAQFISQIRNARHIFLQGA
                     GRSGIAIRAFANRLLHLGFSVSVVGEISSPHTQPGDLLIIGSGSGETTSLKSLAQKAV
                     DSGVNVALVTMKADSTIGKLAQSVLVLPGSVKDDNNRVAGTFAQPMGSAFEQLCFITY
                     DAIVLALMSELGESSATMFTRHADLE"
     gene            complement(5524..5599)
                     /locus_tag="J1138_003850"
     tRNA            complement(5524..5599)
                     /locus_tag="J1138_003850"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5564..5566),aa:Asn,seq:gtt)
     gene            5926..6843
                     /gene="nac"
                     /locus_tag="J1138_003851"
     CDS             5926..6843
                     /gene="nac"
                     /locus_tag="J1138_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HBA8455590.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            6945..7895
                     /gene="cbl"
                     /locus_tag="J1138_003852"
     CDS             6945..7895
                     /gene="cbl"
                     /locus_tag="J1138_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBA8455591.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            7932..8153
                     /locus_tag="J1138_003853"
     CDS             7932..8153
                     /locus_tag="J1138_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455592.1"
                     /translation="MAPLTGIAFASKWLISKLNQSYLPVKTTRMTTNRSWLHKFGLSF
                     PTACGDSYYSVPTWEALILSPSGTVSKER"
     gene            8196..8594
                     /locus_tag="J1138_003854"
     CDS             8196..8594
                     /locus_tag="J1138_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455593.1"
                     /translation="MLINEELVDKIDTKNYGDGSNNVFVIWTLSLKSSTKSECFKFLG
                     FVHLQSVLVEISSSPEKYYKLRIHQERVMEGMMPEEPKAIVIEDTEWGCIHSQYHYQD
                     KDPAIITCIVNVNNTDLIATELVNSVLNTS"
     gene            complement(8684..8884)
                     /locus_tag="J1138_003855"
                     /pseudo
     CDS             complement(8684..8884)
                     /locus_tag="J1138_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8902..9752)
                     /locus_tag="J1138_003856"
                     /pseudo
     CDS             complement(8902..9752)
                     /locus_tag="J1138_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9898..10632)
                     /gene="clbA"
                     /locus_tag="J1138_003857"
     CDS             complement(9898..10632)
                     /gene="clbA"
                     /locus_tag="J1138_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis phosphopantetheinyl
                     transferase ClbA"
                     /protein_id="HBA8455594.1"
                     /translation="MRIDILIGHTSFFHQTSRDNFLHYLNEEEIKRYDQFHFVSDKEL
                     YILSRILLKTALKRYQPDVSLQSWQFSTCKYGKPFIVFPQLAKKIFFNLSHTIDTVAV
                     AISSHCELGVDIEQIRDLDNSYLNISQHFFTPQEATNIVSLPRYEGQLLFWKMWTLKE
                     AYIKYRGKGLSLGLDCIEFHLTNKKLTSKYRGSPVYFSQWKICNSFLALASPLITPKI
                     TIELFPMQSQLYHHDYQLIHSSNGQN"
     gene            complement(10633..10845)
                     /gene="clbR"
                     /locus_tag="J1138_003858"
     CDS             complement(10633..10845)
                     /gene="clbR"
                     /locus_tag="J1138_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis LuxR family
                     transcriptional regulator ClbR"
                     /protein_id="HBA8455595.1"
                     /translation="MDKFKEKNPLSLRERQVLRMLAQGDEYSQISHNLNISINTVKFH
                     VKNIKHKIQARNTNHAIHIANRNEII"
BASE COUNT         3044 a         2111 c         2518 g         3191 t
ORIGIN      
        1 atttttatga tattaaaaac caataaaaac aaataaaaaa taaattaagt caattgagac
       61 aaattgggat tgtacagtgg cgttgtaatg aataataaaa attcatttat tatcaatcgg
      121 atatatggct tttgaactat tgtctttatt gggcttattt gtactttcca cggttagtgt
      181 ttgtttttgt ttgcatcatc tgtgctatgc ttggcgtttt ctcagatggg caatagattt
      241 caggaggaag gatgtatctg gttgatgaaa ggcgaaagtt aatacttgaa gacttaatgt
      301 cggcaggtac ggtctatgtc agtgatctcg caagaaagta tgaagtaacg tatgaaacca
      361 tcaggaaaga tttgacccat cttgaacaaa aagggttgct gatcaaaagc catggtgggg
      421 cgactttaaa gcaaagggcc atagagcatc cctttcaggt cagggaaaaa gagaataact
      481 cttataagaa ggcgattgct cgtaaagcgc ttgattttat tccgcctaat agttcgatga
      541 ttatcggtac gggcagtacc acttttgagc tggctaacct gttatgtatg aagtctgggt
      601 ttaaaatctt cactgattcg ctccccgttg ccagtgtgtt gattgaatct gacaaccagg
      661 tttttctctt tggcggcgaa ctgcgaagaa agagttcctc agtgtttggg gggtgggcgg
      721 tatctatgat caacgatata catgttgatc tctgtttcat tggaaccgac ggctttagta
      781 acatgactgg tccatcaacg ccttcttctt ctgatgcgtt tttggatcgc acgataattt
      841 cgcactcaga caaaaaatat atcctcggag attacaccaa gtttgagcgc agcagtttgt
      901 ttaagatctg cgactggtcg gagatcaccg cccttattac caacgatcat gccgacaaag
      961 aggggattgc cgaagtcgca aaatacacgt cagtaataac ctgctagaac ttactacgac
     1021 acgccttgtc ccttcgaatc agcttagcca aatgtaatca tttcccaatt atcaagtgag
     1081 catccctggt gatgcttact tatcgatctc tattcaattc gtcctctact tattgtcagt
     1141 tttacttcct ttttcgcgaa gaaatcattt tttaattaca caatgctcat atgagcataa
     1201 agattatcgc cattatgtgc ttttttgtga tctggattta atttctcaga tctcgttatt
     1261 tatccacagg atcgttgtgt tataactgct catatgaata tttgttaatg ctcatatgag
     1321 cattgtgggg tgaggggaaa aggatatgat atcgatcagt gttgccgatg gtgcgggact
     1381 acagaactac gatgccgtgc ttgaacatat ttataacacg catcgtgaaa cgaatacggt
     1441 gaaagagagc tggctcaagg ctatgcgtaa acgcgaagcg gaatatccga caggaataga
     1501 actggaaggt tattctatcg ctataccaca ctgtgatagt gaacatgcga gtcaaccggc
     1561 tatttatatc atccgtctgc cagaagcaat acctgtgaac agggctgatg aagatggaat
     1621 attgtgggtc agactcgtcg ttaatcttat tgttacggat cctgctgacc agcttcgatt
     1681 gttaaagtca ttatttaacc atctgcaaaa tgaagaattt taccaaaact tactgacact
     1741 tcccgtcgat gatgcaaaag cactttttgt gtccaaaata atccaatagt aaaaactgga
     1801 gtttattatg attaatattc ttgttgcttg tggtagcggt gtcgctacat ctacgcttgc
     1861 tgctgatgaa gtgaagtcag tatgtgctga atatggtatt acggcattta aaatcaacaa
     1921 atgcagtatg gctgaacttt cttctgagat ggctcatgca gatattgtac tcacaactaa
     1981 taactataaa ggtgatattg ggaaaccaca tatgagcgtt gcagattttg taacgggtat
     2041 taatgaagga gcattacgca agaaattagg agagttatta actgaattag caaatagcta
     2101 aacctgacag gaataataaa aatggaaatg ttgaaaaatt taatggaaac aatcaccgga
     2161 atgggggcga ctgcaatact gccgctggtg atatttattt taggtctggt gttcagaatg
     2221 aaaccaggtg ccgcgatcaa gtcgggcatc acagtcggta ttggttttat tggtttaggg
     2281 ttggttgttg gtttattaaa cagttcatta cagccagcta ttgaatatta ctcaaaagta
     2341 ggtagtggtt ttacggttgc agatattggt tggccagcag tcggtgctgc tgcatgggta
     2401 gcaccttttg ccgcattagt gataccagtt ggcatcgtac ttaacctgat tctggtgcgc
     2461 ctgaaattaa ccaaaacgtt gaatgttgat atctggaact atatgcattt tttagttccg
     2521 ggtgctctgg catattttgt gttcgacagt tttatcatcg gtttctctgt cgcggtggct
     2581 ttgagtattg cggctctgtt tattggcgat ttgattgcgc ccagatggca aaaatattat
     2641 ggactcgagg gaacgacctg taccacgatg attcatatcg gctggactct accgttcgcc
     2701 tgggtagtaa ataaaatcat tgattacatc cccgggttaa ataagttaga tgttgactta
     2761 aacagcgtgc aaaaacgcct gggtgtcttt ggtgaacctg caattatcgg tattatcgtt
     2821 ggcgcattac tcggagtttt aacgaaacag gccattacca caattgttcc catggcgatg
     2881 ggggttgctg gcgtaatggt attgttacct aaagtggtcg gtgtgctgat ggaaggtctt
     2941 aacccgattg ggaaaagtgc caaagaaatc atgcaaaaac agatgggtaa agatgctgaa
     3001 ttaaacatcg gtatggattg tgcactggca ttgggggatc cggcgacggt caccgtgaca
     3061 gtcattacca ttcctttaac catgctatgt gctctggtat tacctgatat taagatcttc
     3121 ccaattggcg tattgatgtc aattatttat atgaccacca tgaccgtaat ggtcagcaat
     3181 ggtaacgtgg ttcgttcgat tatctcgacc ttgttattct gcgttgtagt gatgtattta
     3241 ggcggttatg tcgcaccagg ggcaacgcaa tttttagctg gagccggtgt aggcttgcaa
     3301 ggacaaggta cagattttgt attaaccggc ccgtgggaaa ttttaaccta ttggttgagc
     3361 accgtattac attgatttaa taatctggag tttttaaatg gcaaaaatat taccttctat
     3421 atttggcgcg gatattttac gcctgaaaga tgaaattcat tttctggaag aggaacaaac
     3481 tgaaatattg cacgttgatt taatggatgg cacttacgtc agtaatattg cctttggccc
     3541 aaatcaaatt gcagcaatga aaaaagccag ttcgatgatc tttgatgtgc atatgatgct
     3601 ggcaaatcct gagcgacata ttgatgatgt aatcaatacc ggggcggaga tgatttctgt
     3661 acattatgaa tctacgccac atgtacatta tattattcaa aaaatcaaaa aagcggggcg
     3721 caaagcaggt gttgtgctta atcctgggac acctggatct gtcatcgagt atttattaga
     3781 tgatattgat tatatattgt taatgacaat taaccccggg caaccaggcc agacatttat
     3841 tcataaaagt ctggagaaaa tccgtaatac caaaaaaatg atatctggca gagatattca
     3901 aattgaagtg gatggtggcg ttaatcttga taatgtgaaa caagttaaag aagcgggggc
     3961 tgaccttatt gttgttggcg gtgcattatt taacaaacag cctcatgaaa gttaccaggc
     4021 attaaaaatc gctgttcagt gatcacaatc taaaaatttt acatccagct aagtgaggtt
     4081 ttcgatttca tatcgggagg aaaacctcaa ctaaaaagtc tttaatggag aaaagtaatg
     4141 agtaattttg caggtaaagt ggttttagtt accggaggtg ctcacggtat tggcaaaggg
     4201 atagctaaac aatttgctga tcgtggtgca catactgtta ttgttgacta taacgaagag
     4261 aacggcaagg ccactgcagc agagttaact tttggcgatg ttaaatcgct gtttgttaaa
     4321 acagatgtct ccgatccaca ggcattagtc gatgtacggg aaaaggtaat tgctaccttt
     4381 ggtcgtattg atgtgttgat attaaatgcc ggtgtggcat ttgccaataa agttaacaat
     4441 atttcttttg aggaatggaa taagactatc gcaattaact tatcaggatt atttaatacc
     4501 gttaaggcgt tttatgatga tttcctgaca aataagggat ctattgttta tatctcatcg
     4561 ggatcagcgc tttcggggac tgggggcggt gtgagttatc ctgccagcaa agcgggaggt
     4621 gaagggttga ttcgcggtct ggcaaaggag ttaggaccaa aaggcgtcaa tgttaatgcg
     4681 atcgctcctc gcttaataga caccggagag atgatgcggg tcaattatcc aacacaagcc
     4741 gatttagacg ctgtcctgga aaaaataccc gtccgtcgtc tggggactat cgatgatgtg
     4801 gctaacctgg caatattttt ggcggataaa gataactctt atattcaggg acaaaccatt
     4861 ttaatggatg gtggccggac tattgcttaa aaagggagag aaaaaatgag tgttcaggaa
     4921 aatactatta atattctgca tgaattagag aataatgcgc tgaagattga cgattctcag
     4981 gctgcacagt ttatttcgca aatcagaaat gcccggcata tttttttgca gggagctggg
     5041 cgtagtggca ttgctattcg cgcttttgct aatcgcttgc tgcacttagg tttttcagta
     5101 agcgttgtgg gagaaatatc atcaccgcat acccaacctg gagatctatt aattattggg
     5161 tcaggcagtg gtgaaactac cagtttgaaa agtctggcac aaaaagcagt tgatagcgga
     5221 gtaaacgtcg ctctggtcac tatgaaagct gattcgacga tcggtaaatt agctcaatct
     5281 gtgttggtac taccaggaag tgtgaaagac gataacaatc gtgttgcagg cactttcgcg
     5341 caaccaatgg gctcagcttt tgagcaactc tgctttatta cttatgatgc aattgttctg
     5401 gcgttgatgt cggaactggg tgaaagcagt gccacaatgt ttacccgaca tgctgatctg
     5461 gaatgaccaa taagttggta attagaaagc aaaaagcctg ctcgaagcag gcttttttga
     5521 atttggctcc tctgactgga ctcgaaccag tgacatacgg attaacagtc cgccgttcta
     5581 ccgactgaac tacagaggaa tcgtgtgaac ggggcgcata ttaatggcag tgcatggtga
     5641 tgtcaaaggg tgaaatgcat ttttttgttt gtttgctgcc aatttcaaca aagtggtgag
     5701 ctttcaatct tattgggtaa tgaaccatcg tggtgcatac cctcctttta tagggcaggt
     5761 gaatgcgact gctgataaaa agggccaatg acgaatctat cataatctct tatttgacag
     5821 ttggttagct tgcgcatcaa cgctaaaata gaactggcaa gcatcttgca ttctggttgt
     5881 aagtaatggc ggcacttggg ccgattctta aaaaccggag gcaacatgaa cttcagacgc
     5941 ctgaaatact tcgtaaaaat tgtagatatt ggtagcctga cccaggctgc tgaagtattg
     6001 catatcgcac aaccagcgct cagccagcag gttgccacac tggaaggtga gttaaatcaa
     6061 cagcttttga ttcgcacaaa acggggcgtt acgccaacag acgccggaaa aattctctat
     6121 acccatgcac gggccatttt acgtcagtgt gagcaggccc aactggcggt gcataacgtt
     6181 ggtcagtcat tatcggggca agtctcgatt ggctttgcgc caggaaccgc tgcgtcatcc
     6241 atcaccatgc ccttattaca ggcggttcgc gctgaattcc cggaggtcgt tatctatctt
     6301 catgaaaata gtggtgcagt gcttaacgaa aaactgataa atcaccaact cgatatggcg
     6361 gtgatttatg agcattcccc tgtggctggg gtatccagtc aggctttgct gaaagaagat
     6421 cttttcctgg taggaactca agattgcccg gggcaaagcg ttgatgttaa tgctattgcg
     6481 caaatgaacc tttttctccc cagtgattac agcgctgtta gacttcgtgt tgatgaggct
     6541 ttttccctac ggcggctcac ggcaaaagtc attggtgaaa ttgagtctat tgccacgctt
     6601 accgcagcga ttgccagcgg catgggcgtt gcagtattac ctgaatcggc tgcgcgttcg
     6661 ttatgtggcg cagtaaatgg gtggatgtca cgcattacca ctccttcaat gagtctctct
     6721 ttgtccttaa atttacccgc cagagcaaac ttgtcaccac aggcacaggc agtgaaagag
     6781 ttgttaatgt cagtgattag ttctccagtg atggaaaaaa ggcagtggca attggtgagc
     6841 taagcgttat atcgggatgg aataagatgc gggtttttat tatttgttat gccgggcatt
     6901 agactttaac aataacggga tatctgaact gcccggagtt taccgtgaat ttccaacaac
     6961 taaagataat ccgcgaggct gcacgtcagg attacaacct gacagaggtt gcgaatatgc
     7021 tttttacctc acagtcaggc gttagccgtc atattcggga actggaggat gaacttggca
     7081 tcgaaatatt tgttcgacga ggtaagcgac tgctgggcat gactgaaccg ggcaaagcat
     7141 tactggtcat tgcagaacgt attctgaatg aagccagtaa tgttcgtcgg cttgcagacc
     7201 tgtttaccaa cgatacgtct ggtgttctca ctattgcaac gacgcacact caggcacgtt
     7261 atagcttgcc agaggtcatt aaagcttttc gcgaactttt cccggaggtt cggctcgagc
     7321 taatccaggg tacgccacag gaaattgcga cgttgttgca aaatggcgaa gctgatattg
     7381 gtatcgccag cgagcgtttg agtaatgacc cgcagctcgt cgccttcccg tggtttcgct
     7441 ggcaccatag tttgcttgtt ccactcgatc atcccttaac gcaaattaca ccattgacgc
     7501 tggaatcaat agcgaagtgg ccgttaatta cttaccgaca gggaattacg gggcgctcac
     7561 gcattgatga cgcatttgcc cgcaaaggtt tgccggcaga tattgtatta agtgcacagg
     7621 attctgatgt cattaaaacc tatgttgctc ttgggcttgg gatcggatta gttgccgaac
     7681 aatccagcgg cgaacaagag gaaaagaatt taatccgcct ggatacgcgg catcttttcg
     7741 atgctaatac tgtctggttg ggactgaagc gagggcaact tcagcgtaac tatgtctggc
     7801 gctttctgga actttgtaat gcaggactgt cagttgagga tatcaagcgc caggtgatgg
     7861 aaaacagtga agaggaaatt gattatcaga tatagcaaaa agcccgctat aaaagcgggc
     7921 ttttcagaaa tttggctcct ctgactggga tcgcctttgc cagtaagtgg ctaataagta
     7981 agctaaacca gagttatctt cctgtcaaga ccaccagaat gaccaccaat aggagctggc
     8041 tacataaatt tggtttgagc ttccctactg catgtggtga tagttactat agtgtcccta
     8101 cgtgggaagc gctgatcctc tcccctagtg gaactgtgtc taaagagcga taacagtccg
     8161 ctcagggcgt gaagcggaag ttgctaaatc agtctatgtt aatcaacgag gagctggtag
     8221 ataagattga cactaaaaat tatggtgatg gttccaataa cgtgtttgtt atctggactt
     8281 tatcattaaa atcctctaca aaatccgagt gttttaaatt tctaggcttc gtacatctac
     8341 aatctgtact cgttgaaatt tcttcatctc cagagaagta ctacaagtta cgtatccatc
     8401 aagaacgtgt tatggaaggt atgatgcctg aagaaccaaa agccatagtt atagaagata
     8461 ctgagtgggg gtgtatccac tcgcagtatc actaccaaga taaagatcct gcgataataa
     8521 catgtatcgt aaacgtaaat aatactgact taatagctac agagcttgtg aactcagtgt
     8581 tgaatacatc ctgaatttct tctctcgatg ctggtacaca gcgatcatat aaagttaccg
     8641 cgaaagaaac gactgatcct accctcgtaa tatggacact gctctaagcg aggttctggt
     8701 tttcaaattg ttccggagtg agaccgccac accaactgtg ccgtcgccag cgactgtaat
     8761 cacattcgat ataattaaac accgttgccc gcattatttc ccggctgata aagtgttctc
     8821 catggagaca ttccactttc agtgaatgaa agaagctttc cacgcaggca ttatcgtagc
     8881 agcagtgtaa atagacccat tttagttcca tacacttttt gagatcccgg ccaaataatg
     8941 gcgctgtcgg tattcctccg gtgtcatatt attcagcgat tcatgcgggc gttcacagtt
     9001 atattttgat aaccattttt cagtgatttc ccgtacttca ttcagcgttc tgaacagata
     9061 aaaatcgagt atttctgttc ggtatgtccg gttaaagcgc tcaatgaaag cgttctgcgt
     9121 cggcttaccc ggctggataa actccagttt tattgcatgt ttctctgccc attcagccag
     9181 tgccagggag ataaattccg ggccgttatc cagacgaagc atggccggat agccacggtt
     9241 tgccgcgatc ctgtcgagta cacggaccac tcgctgagct ggcagattca gatcgatttc
     9301 aatcgacaat gcctcacggt taaaatcatc aacgacattg aacatgcgaa aacgacggcc
     9361 acagaccagg gcatcatgca taaaatcgac agaccagctc aggttcagac tccggcaggc
     9421 ctgacggata ctgagttcga acgtcgttat cagatgagta accagctcac gcttaaaggc
     9481 tggttttaaa gctttttttc gataacgtct ttcagcgccc ggttctccag actcaggtcg
     9541 gcaaacattt gttttagacg gcggttctcg tcctcaagat ccttgatttt cttaatatca
     9601 gaagcctcca tgccgccgta tttggacttc cagttgtaat aggtggcttc agagattccg
     9661 gcctcgcgac agacatcttt aacagttcgt ccggcttcaa ccgacttaat cacggcgatg
     9721 atctgatgct cagtaaaacg ggctttacgc atagcgctct ccttcgttgg cagattgatt
     9781 atgccggatg atctctaaat gtgaatggca cgattatgcg ggatacttac accaccgacg
     9841 gaatatgaaa atcaatatta tcgacggctc agaagtgtct agattatccg tggcgattca
     9901 attctgccca tttgacgaat gaattagctg atagtcgtgg tgataaagtt gggactgcat
     9961 aggaaatagc tcaatagtta ttttaggggt gatgagtgga gaggctaatg cgagaaatga
    10021 gttacatatt ttccattgag agaaataaac aggtgaacct ctatattttg aagttagttt
    10081 tttatttgtt aaatgaaatt caatacaatc cagtcctaaa gataggcctt tacctcgata
    10141 tttgatgtaa gcttctttga gcgtccacat tttccaaaaa agtaattgac cttcataacg
    10201 aggaagtgaa actatgttag tagcttcctg tggagtaaaa aaatgctgac tgatattcag
    10261 ataagagttg tctaaatctc ttatttgttc aatatcgaca ccaagctcgc agtgagaact
    10321 aatagcaacg gctactgtat ctatagtatg ggaaaggtta aaaaaaatct tttttgccaa
    10381 ctgaggaaaa actataaatg gtttgccata tttgcacgta ctaaattgcc atgattgtaa
    10441 tgagacatca ggttgatatc tttttagtgc tgttttgagc aggatacggc ttaaaatata
    10501 gagttcttta tcactcacaa aatgaaactg atcatagcgt tttatttctt cctcattgag
    10561 atagtgaagg aagttatctc tactggtttg atgaaaaaaa ctagtatgtc caattaatat
    10621 atcaatcctc atttagataa tctcattcct gttagcaatg tgtatagcgt gattcgtatt
    10681 ccgagcttgt attttatgtt tgatgttttt cacatgaaac tttactgtgt ttattgatat
    10741 gttaagatta tgtgatattt gagagtactc atcaccttgt gccagcatgc gcaatacttg
    10801 tctttcacgc agagataacg ggtttttttc tttgaactta tccatgtttc cccccatcct
    10861 gaat
//
LOCUS       DADTFG010000152        10852 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.153, whole genome shotgun sequence.
ACCESSION   DADTFG010000152 DADTFG010000000
VERSION     DADTFG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.153"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(43..2241)
                     /gene="zntA"
                     /locus_tag="J1138_003859"
     CDS             complement(43..2241)
                     /gene="zntA"
                     /locus_tag="J1138_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBA8455596.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCGGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTLVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGLFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(2315..2941)
                     /locus_tag="J1138_003860"
     CDS             complement(2315..2941)
                     /locus_tag="J1138_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBA8455597.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            3082..3441
                     /locus_tag="J1138_003861"
     CDS             3082..3441
                     /locus_tag="J1138_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBA8455598.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSHKREKPLNDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(3444..3713)
                     /locus_tag="J1138_003862"
     CDS             complement(3444..3713)
                     /locus_tag="J1138_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBA8455599.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(3703..4299)
                     /gene="rsmD"
                     /locus_tag="J1138_003863"
     CDS             complement(3703..4299)
                     /gene="rsmD"
                     /locus_tag="J1138_003863"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBA8455600.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            4449..5945
                     /gene="ftsY"
                     /locus_tag="J1138_003864"
     CDS             4449..5945
                     /gene="ftsY"
                     /locus_tag="J1138_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBA8455601.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            5948..6616
                     /gene="ftsE"
                     /locus_tag="J1138_003865"
     CDS             5948..6616
                     /gene="ftsE"
                     /locus_tag="J1138_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBA8455602.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            6609..7667
                     /gene="ftsX"
                     /locus_tag="J1138_003866"
     CDS             6609..7667
                     /gene="ftsX"
                     /locus_tag="J1138_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBA8455603.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            7912..8766
                     /gene="rpoH"
                     /locus_tag="J1138_003867"
     CDS             7912..8766
                     /gene="rpoH"
                     /locus_tag="J1138_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBA8455604.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            9037..10140
                     /locus_tag="J1138_003868"
     CDS             9037..10140
                     /locus_tag="J1138_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA8455605.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(10328..10711)
                     /gene="panM"
                     /locus_tag="J1138_003869"
     CDS             complement(10328..10711)
                     /gene="panM"
                     /locus_tag="J1138_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBA8455606.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPDVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKH"
BASE COUNT         2678 a         2805 c         2983 g         2386 t
ORIGIN      
        1 acgccgcatc cggcattgtt ggaccgtaga ctgatagcct ctttatttcc tgcgcaataa
       61 ccgtaacgca ttcgccgtca ccagcaccgt cgcacccgta tctgccagca ctgccagcca
      121 tagtccggtc atccctaaca gcgtggtgac gaggaagagc cctttcagcc ccagcgcaat
      181 ggtgatgttc tggcggatat tggcgtgagt ggcgcgtgcc agttcaatca tttgcaccag
      241 gccgcgcagg tggttatggg ttaatgctgc atctgcggtt tccagggcca cgtccgtgcc
      301 actgcccatt gcgatcccaa tggcggcggc tttcatcgct ggcgcgtcgt taataccgtc
      361 gccgaccatc gccagcggcg catgttgatt cagctcggtc accgctttaa ctttatcttc
      421 cggcaagagg cccgctttaa attccagccc cagttccccg gcgattgccg ctgctgcgcg
      481 tggattatcg ccggtgagga tcaccccttt gacacccagc gcgttcagtt cactgatagc
      541 agttgcagca tcggcgcgca gggtatcctg caacgcaatg acacccagca cgtcatcatt
      601 acgtactacc agcactaccg tttgcccggc gctttccagt tcattaatca aaccagcaaa
      661 tgcatcagca ggatgttttc cggcagcgca tatcaacacg cgctcaccgt taacctgcgc
      721 ttcaatgcca gacccgacca gcgcccgctg tgattgggcg gtgggaatgg cgagttcagc
      781 aacctgtgct tcgcgcacaa tggcctgtgc cagtggatgc gtcgcgcctt gctcgaccgc
      841 cgccgccagt gtcagcagtt cagattcact aatacccgtt gccggatgaa tcgcggtaac
      901 gcgcggttta ccgacggtca gcgtaccggt tttatcaaac gccaccaggg taacacgacc
      961 cagctgctcc agcgccgcgc cgcctttaat caacgcccca cgacgcgctg ctgccgccag
     1021 cccggaggta atcgccgcgg gcgtggagat aactaaagca cacgggcaac caatcagcag
     1081 cagcgtcagc cctttataaa tccactcctg ccagctggcg gcaaacagca gcggcggcac
     1141 cagcgttacc agcagggcga cggccataat cgctggcgtg taaatacggc tgaaacggtc
     1201 gataaaccgc tcaataggtg cgcggcgctc ttcggcttct tcaatcagtt tcagaatccg
     1261 gtcaatggcg ctggcacccg gttctgacag cacttccagc gttaccagac ggtctacgct
     1321 ggtggcgcct gcaggaactt tatcgcccgt cgcgcgctcc acaggaatgg attctccggt
     1381 cagggcactt tcatcaaaac tggcaaacgg tgagagcagt ttaccgtcgg caggcaaacg
     1441 cccacctgcc gcaacttcaa tcacatcgcc aggccgcagg ctgtttattg ccacctcttc
     1501 acgctcaccg ttacgcagac gcgtggcggt ttctggcttc agcgccatta acgcgctaac
     1561 gccctgacgc gcgcggctgg cggcccagcc ttccagtcgt tcaccaatca aaaacagcag
     1621 caacaccatc gcagcttcag ccgttgcgcc aataaacagc gcaccaatag cggctacgct
     1681 cattaaggtt tcaatggcga agtagctgcc agatttgatc agccgtaatg cctgacgagc
     1741 aattgggtac agcccaacca gcgtggtcgc gataaacgcc agttgaccaa acggatgatt
     1801 gaactgctcc agcccccaac tgattgccat catcacgata agcgtaatca gcggcagatt
     1861 ctctttcagg cgtgattctt gtggttcgtc ggaggcctgt tcatcgcgca gggaatagcc
     1921 cgctttttgc accgcagatt caacttgtgc ccggatgtca ttgtcggcat ccaccaccag
     1981 tttttcggtg gcgaacaaca cctgtacctg attcacgcct gcaagctggc gcacggcatt
     2041 ttccactttg cgcgcacagg cggcgcagtc cataccgctg actttccagc tatagcgggt
     2101 gccggagacg ttttcagaga gagttggcgt gctggaacat gcgccgccgc agcaacagtc
     2161 gttggtgttc tgtaccgtag ttagcggttt gaacgcagca aattgagggg ctttcttgcc
     2221 gtgattgtca ggagtcgaca tggcatcctc cggttaagtt ttttctcatt aaccgaagga
     2281 tacactctgg agtcgactcc agagtcaagt tttatcagag atacagcgag cggacgatga
     2341 ggaagtgacc agcaaagtag caggccgcgg caatggcgtt atccgcacgg aagcgtcggc
     2401 gatagtgact acccagccag acaaagttac tgataaacag caacgaagcg ccgacaaacg
     2461 cagagagcgc cggagcggtc ggacggaaga accacagttc acctgccagc cacaccatca
     2521 ccagcgtcat gccgataaac gtgcagatag gccaacgata ctcttccaga cgcgtccaga
     2581 taatcgccag taacagcgcg cccagaacca gcagcaccaa cggcagaggc cagaagaaag
     2641 agagcgtcat ctgactggcg aaatagatgg tgtacagcag gtgcgagagg aaaaacgcgc
     2701 cgatggcgta catcagacgt tgacgtggca acagggttag cgcatcgccc agcagtgagg
     2761 cacacagccc cgccagcacc agatagctaa tggcgtcgaa catcggcgct tgccaggcca
     2821 gcaacagcag gagaagaagg gttaacggtt taaacaccca gcgttgccag gttggcccac
     2881 gatacgatgc atccacagat agccatgcgg aaagacagac agcgataaac gaccaaagca
     2941 tcttagttcc ttgaattcgt tattttttcg taagcggaat gcctacggtc ttgtttccag
     3001 tttagtggcg agggtggacg gatgacaaca ccataactgg cagggtatgc ttatttccgc
     3061 attttccgat gagggataaa gatgagtaaa ccacctcttt tctttattgt tatcattggc
     3121 ttaatcatcg tcgccgcatc gtttcgtttt atgcaacaac gacgggaaaa agctgataat
     3181 gatatggcac cgctccagca aaagctggtg gtggtgagcc acaagcggga aaaaccgctt
     3241 aacgatcgcc gttcgcgcca gcaggaagtg actccggcag gcaccagtat gcgctatgag
     3301 gcaagcttca aaccgcaaag cggaggaatg gagcagacgt ttcgcctcga cgcccagcag
     3361 taccacgccc tgacagtggg tgataaaggt acgctgagct ataaaggaac gcgctttgtc
     3421 agctttgtag gcgaacaata aggttatttt ttaactttaa atttcttctg ccagaccagc
     3481 agttcaaaca cgccgaaaag gaaaatccgc accttttcgg cggtggtcat ctgcgggcca
     3541 tctttcggta aagtggattt caatagcgcc agctgcatac catgcatcag cacggtaaaa
     3601 atcagcgcca cgttaacgaa aatattcagc gggcgtggga agggatgcac caggttgagg
     3661 attaaaaatc cccaaacaca gagcattaac aaacgaccaa tattaatcag catcactttc
     3721 tccttgtgct tcgcgttgat acagccgata agccacctga cccgccactt tttcccgatg
     3781 taatgaccag tttgccggaa cagtgggcag accgttttcg acttcgcttt cgacataaat
     3841 cagggcttcg tcagccagcc agccgttatc ttccagtaaa tttatcgtct cttctaacaa
     3901 gccacggcgg aacggtggat cgacaaacac gatattatgc ggtgtgcctt tttgcgccag
     3961 gaatgacatc gcgttgctgt tcaccacgcg tgcattgcct gcttttagtg tcgccagatt
     4021 cttaattaac tgctgagaaa ccgcacgatc catctcaatc aacgtggccc ccgcagcgta
     4081 gcgcgataac gcttccagcc ccagcgcgcc gctcccggcg aagcaatcca gacattgggc
     4141 gtcaacaatg accggagcca gccagttaaa caacgtttcg cgtacgcggt cggtggtggg
     4201 gcgcagacct ggactatcag gaaccgggag tttacggcct cgccactgcc cgccaataat
     4261 gcggatttgg ccgctgccgg aatgattcgg ttttttcatg ataaattgct caatccgcca
     4321 gataacacat acttgcaggc ggtgatgtga attaagttaa gtgatagact atttcatcat
     4381 ttttttagct gctatgtaca tagcgttaac gctgtgccat gaagcaacag cgaggagtgt
     4441 agtcgcaaat ggcgaaagaa aaaaaacgtg gctttttttc ctggctgggc tttggtcaaa
     4501 aagagcagac cccggaaaaa gagacagaag ttcagaatga acaaccagtt gtagaagaaa
     4561 tcgttcaggc gcaagagcct gtgaaggctt ctgaacacgc cgttgaagag cagccgcagg
     4621 cgcatactga agccgaggcg gaaacttttg ctgccgacgt tgtggaagtt actgaacagg
     4681 ttgctgaaag tgaaaaagcg cagcctgaag cggaagtcgt tgcacagccg gaaccggtcg
     4741 tagaagaaac gccggagcca gtggctatcg aacgtgaaga actgccgttg ccggaagacg
     4801 tcaacgccga agcggtttcg ccagaagagt ggcaggccga agcggaaacc gtagagattg
     4861 tcgaagcggc ggaagaagaa gcggctaaag aagaaatcac cgacgaagag ccggaggcac
     4921 aggcgctggc tgccgaagcg gcagaagagg cggtaatggt ggtttctccg gcagaagaag
     4981 agcagccggt ggaagaaatc gctcaggagc aggaaaaacc gaccaaagaa ggtttcttcg
     5041 cgcgcctgaa acgcagcctg ttaaaaacca aagaaaatct cggttccgga tttatcagcc
     5101 tgttccgcgg taaaaaaatc gacgatgatc tgtttgaaga gctggaagaa cagctgttga
     5161 tcgccgatgt gggcgtggaa accacacgta aaattatcac caatctgaca gaaggcgcat
     5221 cccgcaagca gcttcgtgat gccgaggcgc tctatggcct gctgaaagaa gagatgggcg
     5281 agattctggc gaaagtcgat gagccgctga atgttgaagg taaaacgccg ttcgtgatcc
     5341 tgatggtggg cgtcaacggt gtgggtaaaa ccacgacgat tggtaagctg gcgcgtcagt
     5401 ttgagcagca gggtaaatcg gtgatgctgg cggcaggcga tactttccgt gcggcagcgg
     5461 ttgaacagct tcaggtctgg ggtcagcgca acaatattcc ggtgattgcc cagcataccg
     5521 gtgctgattc cgcttctgtt atcttcgacg ccattcaggc cgctaaagcg cgtaacatcg
     5581 acgtcctgat tgccgatacc gccggacgcc tgcaaaacaa atcgcacctg atggaagagt
     5641 tgaagaaaat cgtccgcgtg atgaagaaac tcgacgttga agctccgcat gaagttatgc
     5701 tgactattga tgccagcacc gggcagaatg cggtaagcca ggccaaactg ttccacgaag
     5761 ccgttggctt aaccggcatc acgctaacga aactggacgg cacggcgaaa ggcggggtaa
     5821 ttttctcggt ggctgaccag tttggtatcc ctatccgcta cattggtgtc ggcgaacgta
     5881 ttgaggattt gcgtccgttt aaggcggacg actttataga ggcacttttt gcccgagagg
     5941 attaacaatg attcgctttg aacatgtcag caaggcttat ctcggtggga gacaggcgct
     6001 gcagggcgtt acgttccata tgcagccggg tgagatggcg tttctgaccg gtcattccgg
     6061 cgcagggaaa agtaccctcc tgaagctgat ctgtgggatt gagcggccca gcgccgggaa
     6121 aatctggttt agcggccatg acatcacgcg tctgaaaaac cgtgaagttc cgtttctgcg
     6181 ccgccagatt ggcatgattt tccaggatca ccatctactg atggaccgta ctgtctatga
     6241 taacgtggcg atcccgctga ttatcgccgg tgccagcggt gatgacattc gtcgccgggt
     6301 gtcggcggcg ctggataaag tcgggctact ggacaaagcg aagaacttcc ctattcagct
     6361 ttccggcggt gaacaacagc gtgttggcat tgcccgcgcg gtggtgaaca agcccgcggt
     6421 actgctggcg gacgaaccga ctggtaacct ggacgacgcg ctgtcggaag gcattttacg
     6481 tctgtttgaa gagtttaacc gcgttggagt aaccgtattg atggcaacgc acgacatcaa
     6541 cctgatttcg cgacgttcct atcgcatgct caccctgagc gatggtcact tgcatggagg
     6601 cgtgggccat gaataagcgc gatgcaatca atcacattcg gcagtttggc gggcgtctgg
     6661 atcgcttccg taaatcggtc ggcggctctg gcgacggcgg tcgtaacgcg ccgaaacgcg
     6721 cgaaatcctc gccaaaaccg gtaaatcgca aaaccaacgt tttcaacgaa caggtacgct
     6781 atgccttcca cggcgcattg caggatctga aaagcaaacc gttcgccacg tttttaacgg
     6841 tgatggttat cgccatctcc ctgacgctgc ccagcgtctg ttatatggtg tacaaaaacg
     6901 ttaaccaggc ggcgacgcaa tattatccgt caccgcaaat cactgtttat ctgcaaaaaa
     6961 cgctggatga tgacgccgct gcgggcgtgg tggcacagtt gcaggccgag caaggcgtgg
     7021 agaaagtgaa ttatctttct cgtgaagacg cactgggcga gttccgtaac tggtctggtt
     7081 ttggtggtgc gctggatatg ctggaagaaa acccgcttcc ggcagtggcg gtggtgatcc
     7141 cgaaactcga ttttcagggg acggagtcac tgaatacgct gcgtgatcgt atcacgcaga
     7201 ttaacggcat tgacgaagtg cggatggatg acagctggtt tgcccgcctg gcggcgttga
     7261 ccgggctggt cgggcgcgtt tcggcaatga tcggcgtatt gatggtggcg gcagtgttcc
     7321 tcgtcatcgg taacagtgtg cgcctgagta tttttgctcg ccgtgattct attaacgtgc
     7381 agaaactgat tggtgcgacg gatggtttca tcctgcgtcc gttcctgtac ggtggcgcat
     7441 tgctgggatt ttctggcgca ttgttgtcac taattttgtc agaaattctg gtgctgcgtt
     7501 tgtcatcggc ggttgcggaa gtggcacagg ttttcggaac gaagtttgat atcaatggct
     7561 tatcgttcga tgaatgcctg ctattgctgc tggtatgctc gatgattggc tgggtggcag
     7621 cgtggcttgc cacggtacaa catttacgcc actttacgcc tgaataataa aagcgtgtta
     7681 tactctttcc ctgcaatggg ttccgtagca gggaaagaga ccccgttgtc tcttcccggt
     7741 atttcatctc tatgtcacat tttgtgcgta atttattcac agcgttgcat tgaacttgtg
     7801 gataaaatca cggtctgata aaacagtgaa tgataacctc gttgctctta agctctggca
     7861 cagttgttgc taccactgaa gcgccagaag atatcgattg agaggatttg aatgactgac
     7921 aaaatgcaaa gtttagcttt agccccagtt ggcaacctgg attcctacat ccgggcagct
     7981 aacgcgtggc cgatgttgtc ggctgacgag gagcgggcgc tggctgaaaa gctgcattac
     8041 catggcgatc tggaagcagc taaaacgctg atcctgtctc acctgcggtt tgttgttcat
     8101 attgctcgta attatgcggg ctatggcctg ccacaggcgg atttgattca ggaaggtaac
     8161 atcggcctga tgaaagcagt gcgccgtttt aacccggaag tgggtgtgcg cctggtctcc
     8221 ttcgccgttc actggatcaa agcagagatc cacgaatacg ttctgcgtaa ctggcgtatc
     8281 gtcaaagttg cgactaccaa agcgcaacgc aaactgttct tcaacctgcg taaaaccaag
     8341 cagcgtctgg gctggtttaa ccaggatgaa gtcgagatgg tggcccgtga actgggcgta
     8401 accagcaaag acgtacgtga gatggaatca cgtatggcgg cacaggacat gacctttgac
     8461 ctgtcttccg acgacgattc cgacagccag ccgatggctc cggtgctcta tctgcaggat
     8521 aaatcatcta actttgccga cggtattgaa gatgataact gggaagagca ggcggcaaac
     8581 cgcctgaccg atgcgatgca aggtctggac gagcgtagtc aggatatcat ccgcgcgcgc
     8641 tggctggatg aagacaacaa gtccacgttg caggaactgg ctgaccgtta cggtgtttcc
     8701 gctgagcgtg tgcgtcagct ggaaaagaac gcgatgaaaa aattgcgcgc tgccattgaa
     8761 gcgtaatttc cgctattaag cagagaaccc tggatgagag tccggggttt ttgttttttg
     8821 ggcctctaca ataatcaatt ccccctccgg caaaacgcca atccccacgc agattgttaa
     8881 taaactgtca aaatagctat tccaatatca taaaaatggg gtatgtttta gcagagtatg
     8941 ctgctaaagc acgggtagct acgtataaaa cgaaataaag tgctgcacaa caacatcaca
     9001 acacacgtaa taaccagaag agtggggatt ctcaggatga acataaaggg taaagcgtta
     9061 ctggcaggat gtatcgcgct ggcattcagc aatatggctc tggcagaaga tattaaagtc
     9121 gccgtcgtag gcgcaatgtc cggtccggtg gcgcagtacg gtgaccagga gtttactggc
     9181 gcagagcagg cggttgcgga tatcaatgct aaaggtggta tcaaaggcaa caaactgcaa
     9241 atcgtaaaat atgacgatgc ctgtgacccg aaacaggcgg ttgcggtggc gaacaaagtc
     9301 gttaacgacg gcattaaata cgtgattggt cacctctgct cttcatcaac gcagccagcg
     9361 tctgacatct acgaagacga aggcatttta atgatcaccc cagcggcaac cgcgccggag
     9421 ctgaccgccc gtggctatca gctgatcctg cgcaccaccg gcctggactc cgaccagggg
     9481 ccgacggcgg cgaaatatat tcttgagaaa gtgaaaccgc agcgtattgc tatcgtccac
     9541 gacaaacagc aatacggcga gggtctggcg cgtgcggtgc aggacggcct gaagaaaggc
     9601 aatgctaatg tggtgttctt tgatggtatt accgccgggg aaaaagattt ctcaacgctg
     9661 gtggcgcgtc tgaagaaaga gaatatcgac ttcgtttact acggcggtta ccacccggaa
     9721 atggggcaga tcctgcgtca ggcacgtgcg gcagggctga aaacccagtt tatggggccg
     9781 gaaggcgtgg ctaacgtctc gctgtctaac attgcgggtg aatcagcaga agggctgctg
     9841 gtgaccaagc cgaagaacta cgatcaggtt ccggcgaaca aaccgattgt tgacgcgatc
     9901 aaagcgaaaa aacaggaccc aagcggcgcg ttcgtctgga ccacctacgc cgcgctgcaa
     9961 tctttgcaag cgggcctgaa tcagtctgac gatccggcgg agatcgctaa atacctgaaa
    10021 gcgaactcgg ttgatacggt gatggggccg ctgacctggg atgagaaagg cgatctgaaa
    10081 ggctttgagt tcggtgtgtt tgactggcac gccaacggca cggcgactga tgcgaagtaa
    10141 tcattaatcg gcaactttgg gttgccgcca aattgctaat atcgagtacg ttgcttcatg
    10201 ccggatgcgg cgtaaacgcc ttatccggcc tacaagattc aaagaaatca gtaaattgca
    10261 gcacacattg taggcctgat aagcgtagcg catcaggcaa tatcactttt gaaatcaaac
    10321 ttgacgatta atgcttctcc cagccaccct gttgtgccgt aaatcccagt gctcgcataa
    10381 acgctgtcat cacgccgcga tcttccacgc cagcatccgc catccaccaa catgaaacgt
    10441 caggattgtt acgcaaaacc tcttccagta gatattgccc cacaccgcga cgtcgggtga
    10501 cttcccgcac gcgtagagaa tccagagccc cctcggtgcc gcttaaggtt acccgcacgg
    10561 cagcgagcag gcgctcgtta aaacgcgcgg cgtagatacg gtggttatcg tcaacctgta
    10621 aagaggaagg ggaatactcc ggccagatct tttgcaggtc aatccggtcc tggtcgctaa
    10681 agttttctaa tcgaatgatg gtcagcttca tggggaaccc gtgtaaatca caaaagtgga
    10741 accagtgtag cgaaataatt taatcggagg ctttctcttt tttatttctt ttggcaggtg
    10801 attaattttt tttcagcaat aattacaaag ttaaaacatt agagaatgaa aa
//
LOCUS       DADTFG010000153        10709 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.154, whole genome shotgun sequence.
ACCESSION   DADTFG010000153 DADTFG010000000
VERSION     DADTFG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.154"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(80..1318)
                     /gene="cca"
                     /locus_tag="J1138_003870"
     CDS             complement(80..1318)
                     /gene="cca"
                     /locus_tag="J1138_003870"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBA8455607.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(1382..2002)
                     /locus_tag="J1138_003871"
     CDS             complement(1382..2002)
                     /locus_tag="J1138_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBA8455608.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            2244..3545
                     /locus_tag="J1138_003872"
     CDS             2244..3545
                     /locus_tag="J1138_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBA8455609.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            3568..6408
                     /gene="glnE"
                     /locus_tag="J1138_003873"
     CDS             3568..6408
                     /gene="glnE"
                     /locus_tag="J1138_003873"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBA8455610.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNTQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRDLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELTRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLMGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFNAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            6456..7889
                     /gene="hldE"
                     /locus_tag="J1138_003874"
     CDS             6456..7889
                     /gene="hldE"
                     /locus_tag="J1138_003874"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBA8455611.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQLDKKG"
     gene            complement(8009..8068)
                     /gene="ibsE"
                     /locus_tag="J1138_003875"
     CDS             complement(8009..8068)
                     /gene="ibsE"
                     /locus_tag="J1138_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBA8455612.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8308..9969)
                     /locus_tag="J1138_003876"
     CDS             complement(8308..9969)
                     /locus_tag="J1138_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBA8455613.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            complement(9996..10625)
                     /locus_tag="J1138_003877"
     CDS             complement(9996..10625)
                     /locus_tag="J1138_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HBA8455614.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTFWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWSQIL"
BASE COUNT         2479 a         2707 c         2954 g         2569 t
ORIGIN      
        1 cgtcttatca ggcctacaac tgtcgttgac atgtagccgg ataagacgct tgcgtcgcat
       61 ccggcaaaat atccaacact cactcaggtt ttgggcaacg ctgttccttc cagctggcta
      121 ccgccgcaat ccgccgtcgg gtcagctcct cacgaatctc cacaccttta aatcctgctt
      181 caacgacagc ttttgtcggc actgactgcg ccacttccca ggcttcgcgc aaccagcggc
      241 cttgcgggta gtccgccgat tcaaaaccgg ttctgccgcg cacgtcagcc tcgctggtca
      301 gcgccagttg ctcgacacgc tgcggtttac gccaggcatc aatggaatca aacaatttaa
      361 caatggtttt cgggttcagc attggaaagg tgtggatgag atcgtgaaac tcagctacca
      421 gtctggctaa atcgcgaatt tcatttggca cgcgcagacg ctggcatagt tgttccacta
      481 acttcacacc cgccgggcca tgaccatgat gacgtggcca aagttctggc ggcgtcagtc
      541 ctttaccgag atcgtggcat aacgttgcga aacggacatc gacctgcgga ctcagcatcg
      601 ccgccattga gagcgtcatt aaggtatgaa tacccgtgtc gatttccgga tgccacctgg
      661 caggggccgg aacgccaaac agggcgtcaa tttccgggaa taaaacgcgc agtgcgccgc
      721 aatcgcgcag tacctggaag aacacctggg gattgcgggt ggtgagggcg ttttccgtct
      781 ctttccatac ccgttcaggc gtcaggtgtt ccagttcacc cgcatgggtc atctcgcgca
      841 tcagcgccag agtttcatcg gcaatacgga aactgaggtg ggcataacgc gcagcaaaac
      901 gcgctacgcg caacacgcgt aacggatctt cgccaaaagc gggggaaaca tggcgcaaca
      961 gacgattttg cagatcgccc agaccgttgt acgggtcgat aatctcgccg ttatcgtcct
     1021 gggccagcgc attaatggtc agatcgcgac gcttaagatc atcttccagc gtgacatccg
     1081 gtgcggcata gcaggtaaaa ccggtgtaac cggaaccgga tttccgttca gtacgtgcca
     1141 gcgcatactc ttcatgcgtt tgcggatgca gaaaaacagg aaaatcgcgg cctacctgct
     1201 ggtagcccgc gtcgagcatc tcctgtggcg tactgccgac caccacccaa tctctgtctt
     1261 tgaccggtag ccctaacaat gcatcccgaa cagcaccacc gaccagataa atcttcacgc
     1321 catcatccct tcgtcaaata cacaattcct aataataaga cagtgcaacg aagaaggcga
     1381 tttagttcat ccagcgatct ttgcgtttgc ggcttgggat caggtgcggc agtaccagac
     1441 cgagcagcaa gccaagcccc agcacgccgc cgccatacat aaaccattgc atgatgatgg
     1501 tgcgctgttt gtcatccagc tgtacgctgg cggcatcgac ctttttctgt gcgacaatca
     1561 gctcgttttt cagtttctga ttttcttctt ttaacccgtt gatcacgctg tcgctctgcg
     1621 ccactttttg ctgcatttct gcggtgcgct gattccaggt gttatcgata ttggtgagtt
     1681 tatcggtcag ggttttgacc tgattttcca gatctggcac acgggagcgc aggcttggct
     1741 cagtgctaag ttgtttcaac gggatccagg cggtacggcc agagctgtct ttcacttggg
     1801 cataattggt gttggcgtca gtttgtaata aggtcacttc ctcgccggcg ttaaccgtgc
     1861 ccacgaggcg ataatgatct cccggaccgc tacggaccca ggtattcagt tcatcagaaa
     1921 catagcgcgt ttcttcagcg tgtgagacgg cagtggcgct aagtgcgagt aaagttaatc
     1981 cgatcaggcg taattttggc atcaggctgt cgttattgtc atggaaagtg gaacgatagt
     2041 agtggcatca gtgtcgctac gcaaagcatt cgacatcaat cggaatcctc tgtgtcaggt
     2101 cttgccactg cttcaaactt ttacgcccgt caaattgccc gttgcatggc aatttggcgc
     2161 aaaatactat ctactgacaa aaaagatcgc attaagttcg ccgttcatcg tcacttatgt
     2221 gacataaccg ataagtaaag gctatggctc aggaaatcga attaaagttt attgttaatc
     2281 acagtgccgt tgaggcgttg cgtgaccatc tcaatacgct gggcggcgag caccatgatc
     2341 ccgtgcagtt gctgaatatt tactacgaaa cgccggataa ctggctgcgt gggcacgaca
     2401 tgggcttgcg tattcgtggc gaaaacggtc gctatgagat gaccatgaaa gttgcaggaa
     2461 gagtgacagg cggcttacac cagcgcccgg aatataacgt ggcgttgagc gaaccgacgc
     2521 tcgacctggc gcagttaccg acggaagtct ggccgaacgg cgaattgcct accgatctcg
     2581 cttcccgcgt gcagccgctg tttagcaccg atttttatcg cgaaaagtgg ctggtagagg
     2641 ttgatggtag ccaaattgaa atcgccctcg accaggggga agtgaaagcg ggtgaatttg
     2701 ctgaacctat ctgcgagctg gaactggaac tgcttagcgg cgacacgcgc gcggtgctga
     2761 aactggcgaa ccaactggta tcgcaaaccg gattacgcca gggcagcctg agcaaagcag
     2821 cgcgtggcta tcatctggcg cagggcaatc cggcgcgtga aatcaaaccg accaccattt
     2881 tgcatgttgc ggcaaaagct gatgtggaac aggggctgga agcggcgttc gagctggcgt
     2941 tagcgcaatg gcagtatcat gaagaactgt gggtacgcgg caacgatgcg gcgaaagagc
     3001 aggtgctggc agccattggc ctggtccgtc atacactgat gctgttcggc ggtatcgtgc
     3061 cgcgtaaagc gagcactcac ttacgtgatc tgctgactca atgcgaggcg accattgctt
     3121 ctgcggtttc tgccgtaacg gcggtttact ctaccgaaac ggcaatggcg aagctggcgt
     3181 tgaccgaatg gctggtaagc aaagcctggc agccgttttt agatgccaaa gcgcagagta
     3241 aaatgagcga ttccttcaaa cgctttgccg atatccatct ttcccgccat gctgctgaac
     3301 ttaaaagcgt tttctgccag ccgttaggcg atcgctaccg tgaccaattg ccacgcctga
     3361 cgcgtgatat tgactcaata ctgttgctgg cgggttacta cgatcctgtc gtcgcgcaag
     3421 cctggctgga gaactggcag gggctgcgtc acgctattgc gaccgggcaa cgcattgaaa
     3481 ttgaacattt ccgtaatgag gcaaacaatc aggaaccgtt ctggttgcac agcggaaaac
     3541 gttaatcagg caaggataaa atcgcttatg aagccgctct cttcaccgtt acagcagtac
     3601 tggcagaccg ttgttgagcg gctgccagag cctttagccg aggaatcact tagcgcacag
     3661 gcgaagtcag tacttacttt cagtgatttt gtgcaggaca gcgtgattgc gcatccagag
     3721 tggctgacgg aactggaaag ccaaccgccg caggccgacg aatggcagca ttacgctgca
     3781 tggttgcagg aggcgctcag taatgtgagt gacgaagccg ggttaatgcg cgagctgcgg
     3841 ctattccggc ggcgcattat ggtgcgcatc gcctgggcgc aaacgctggc tctggttact
     3901 gaagagagca tattgcagca gctcagccat ctggcggaga cgctgattgt tgcggcgcgt
     3961 gactggctgt atgacgcctg ctgccgcgaa tggggaacgc cgtgcaatac gcagggcgaa
     4021 gcgcaaccgc tgctgatttt aggcatgggt aagctgggcg gcggggaact gaatttctcc
     4081 tctgatatcg atctgatttt tgcctggccg gaacatggtt gtacccaggg tggacgccgg
     4141 gaactggata acgcgcagtt ttttacccgc atggggcagc ggctgattaa agtgctggat
     4201 caaccaacgc aggatggttt cgtctatcgc gtggatatgc ggctgcgtcc gtttggtgaa
     4261 agtggcccgc tggtgctgag ttttgcggcg ctggaagatt attaccagga gcaggggcgc
     4321 gactgggagc gttacgcgat ggtcaaggcg cggattatgg gggatagcga cggcgtctat
     4381 gctaacgagt tgcgtgcgat gctgcgtccg tttgttttcc gtcgttacat cgatttcagc
     4441 gtgattcagt cgctgcgcaa catgaaagga atgattgccc gtgaagtgcg tcgacgcgat
     4501 ttgaccgaca acatcaaact tggcgcaggc ggcattcgcg aaattgaatt tatcgtccag
     4561 gtgttccagc tcattcgcgg cgggcgcgaa ccgtcgctgc aatcgcgctc tttactgcca
     4621 acgctcagcg ccattgccgc gctgcatctg ctttctgaaa acgatgctga acaattgcga
     4681 gtggcgtatc tgttcctgcg gcgtctggaa aacctgctgc aaagcattaa cgacgaacaa
     4741 acccagacgc ttccttctga tgagcttacc cgtgcgcggc tggcgtgggc gatggacttt
     4801 gctgactggc cgcaattgac cggggtgctg accgcacata tggccaatgt gcgccgggtg
     4861 tttaatgaat tgattggcga cgacgaaagt gaaactcagg aagagtcgct gtcggaacag
     4921 tggcgtgagc tgtggcagga tgcgttgcag gaagatgaca ccacgccagt gctggcgcat
     4981 cttagcgagg atgatcgcaa acaggtgcta atgctgattg ctgattttcg caaagagctg
     5041 gataagcgca ccatcgggcc gcgaggacgt caggtgctcg accatctgat gccacatctg
     5101 ctaagtgatg tctgtgcgcg tgaagacgct gctgtcacgc tgtcgcgcat taccgccttg
     5161 ctgatgggga ttgttactcg caccacttat cttgagctgc ttagtgaatt ccctgcggcg
     5221 cttaaacatt tgatttctct gtgtgctgca tcgccgatga ttgccagcca gctagcgcgt
     5281 tatccattat tgctggatga attgctcgat ccaaataccc tctaccaacc aacggcgacg
     5341 gatgcctacc gtgatgaact gcgccagtat ttgctgcgcg tgccggaaga tgacgaagaa
     5401 caacagcttg aggcgctgcg tcagttcaaa caggcgcagc tgttacgcat tgcggcagcg
     5461 gatatcgccg gtacgctacc ggtaatgaaa gtgagcgatc acttaacctg gctggcagaa
     5521 gccatgattg atgccgtcgt tcagcaggcg tgggttcaaa tggttgcccg ctacggtaag
     5581 ccaaatcacc tgaacgaacg cgaagggcgt ggttttgcgg tggtcggcta cggcaagctg
     5641 ggtggctggg agttaggcta cagttccgat cttgacctga tcttcctcca tgattgccca
     5701 atggatgcga tgactgacgg cgagcgggaa atcgacgggc gacagtttta tctgcgtctg
     5761 gcgcaacgca ttatgcatct gttcagtacg cgaacctctt ccggcatttt gtatgaagtg
     5821 gatgcacgac tgcgtccgtc cggggcggcg ggaatgctgg tgacatccgc agaagcattt
     5881 gctgattatc agaaaaacga ggcctggacg tgggagcatc aggcgctggt gcgtgcgcgt
     5941 gtagtgtacg gcgatccgca gctcaccgcg cactttaacg cagtacgccg ggagattatg
     6001 acgctgccgc gcgaaggcaa aaccctgcaa acagaagtgc gggaaatgcg cgagaaaatg
     6061 cgtgcccatc tcggcaataa acatcgcgat cgctttgata tcaaagccga tgaaggcgga
     6121 attaccgata tcgaatttat tactcaatat ctggtgttgc gctacgctca cgaaaaaccg
     6181 aagttaacgc gctggtcaga caacgtgcgc attctggaac tactggcgca aaacgacatt
     6241 atggaagagc aggaagcgat ggcgctgacc cgtgcttaca cgacgctgcg cgatgaactt
     6301 catcatctgg cattacagga attgccgggc catgtgtcgg aggattgctt caccgcagag
     6361 cgtgaactgg tgcgggcaag ctggcagaag tggctggtgg aagaatgaag tatggtatta
     6421 tcgcgcgcaa attttgaatc tctcaggaga caggaatgaa agtaacgctg ccagagtttg
     6481 aacgtgcagg agtgatggtg gttggtgatg tgatgctgga tcgttactgg tacggtccca
     6541 ccagtcgtat ctcgccggaa gcgccggtgc ccgtggttaa agtgaatacc atcgaagaac
     6601 gtccgggcgg cgcggctaac gtggcgatga atatcgcttc tctcggtgct aatgcacgcc
     6661 tggtcgggtt gacgggcatt gacgatgcgg cgcgcgcgct cagtaaatct ctggccgacg
     6721 tcaacgtcaa atgcgacttt gtttctgtac cgacgcatcc gaccatcacc aaattacggg
     6781 tactttcccg caaccaacaa ctgatccgtc tggatttcga agaaggtttc gaaggggttg
     6841 atccgcagcc gctgcacgag cggattaatc aggcactgag ttcgattggc gcgctggtgc
     6901 tttctgacta cgccaaaggt gcgctggcaa gcgtacagca gatgatccaa ctggcgcgta
     6961 aagcgggtgt tccggtgctg attgatccaa aaggtaccga ttttgagcgc taccgcggcg
     7021 ctacgctgtt aacgccgaat ctctcggaat ttgaagctgt tgtcggtaaa tgtaagaccg
     7081 aggaagagat tgttgagcgc ggcatgaaac tgattgccga ttacgaactc tcggctctgt
     7141 tagtgacccg ttccgaacag ggtatgtcgc tgctgcaacc gggtaaagcg ccgctgcata
     7201 tgccaaccca ggcgcaggaa gtgtatgacg ttaccggtgc gggcgacacg gtgattggcg
     7261 tcctggcggc aacgctggca gcgggtaatt cactggaaga agcctgcttc tttgccaatg
     7321 cggcggcagg cgtggtggtc ggcaaactgg ggacatccac ggtttcgccg atcgagctgg
     7381 aaaatgcagt acgtggacgt gcagataccg gctttggcgt gatgaccgaa gaggaactga
     7441 agcttgctgt agcggcagcg cgtaaacgcg gtgaaaaagt ggtgatgacc aatggcgtct
     7501 ttgacatcct gcacgccggg cacgtctctt atctggcaaa tgcccgtaaa ctgggtgacc
     7561 gtttgattgt cgctgtgaac agcgatgcct ccaccaaacg gctgaaaggg gattcccgcc
     7621 ccgttaaccc gctcgaacag cgtatgattg tgctgggcgc actggaagcg gtcgactggg
     7681 tggtgtcgtt tgaagaggac acgccgcagc gcttgatcgc cgggatcttg ccagatctgc
     7741 tggtgaaagg cggcgactat aaaccagaag agattgccgg gagtaaagaa gtctgggcca
     7801 acggtggcga agtgctggtg ctcaactttg aagacggttg ctcgacgacc aacatcatca
     7861 agaagatcca actggataaa aaaggctaac tggaaagcgg ttcacagatc tcgcttcgta
     7921 gcaggcaggc ctattgacgc tgttgttttt tcaacctaaa gtaaaggaac aagggtaagg
     7981 gaggatttct cccccctctg atgagttgtt agtaagtcgg gaaacttaac agtaacaaca
     8041 caaccagtat gatgacgaac ttcatcataa ccctttcctt ctataaggcc ccttcctcgg
     8101 gaggggcttt cccgtttcag ccccttgctg actccccgat tgttgatgag cgccggaccc
     8161 cgcgcaacgc tttcccagag gcagttttac cttccccgag cagtcacgca aaatgctgca
     8221 acagcctgat catttgcacc ttttagcgga acgcagatat aaaaaatccc cggctttttg
     8281 ggccagggat tattcataga agagggttta ctctgctttt tcttcgacgg gagttgttga
     8341 gcttaaggat gaagtcaatg ccgccagtga gccgcctgaa acgccaattt cattgagcaa
     8401 ggagtcaatc agcggtgcct gtgtgcggta agagagtgcg gctgataacg cttgttctgc
     8461 caggtttccg ccccccacat tacccgtgtt tgcatcaccc gtagcgccac cacgattcag
     8521 cccatcgacc tgaataattt tgatgccgtc gatagacttc attggttcca cggatttctc
     8581 aattaccgca ggcagcgcct gcaataatgc cagtttgaat ttaaggctgg tttgttcatc
     8641 agaaagtacg ttgatagcat cgttcagcgc acgttgtgct tctgcttccg ccagaccctt
     8701 tttacgtgta gcttcggcta actcaacgat agccgccgcc tgcatttctg cggcttcttt
     8761 ttctgctttc gcccgcacgg tcagttcaac cgctttggtt tctgcatcct gcgctgcggc
     8821 gatcaacgcc acttgtttag cacgatcggc ttcggcagtc tggcgagtgg tttctacgtt
     8881 ttgttgggcg cttactgctt ctgcgagtgc aaggttagca cgcgcttctg cctgggactg
     8941 ttgttccgat ttggcggcaa tagcgatcga tttcgtctgg ttggcgattt cggtgacctg
     9001 ctgctgttcg atctctttaa tgcgaacttc acgttcagct tcaacttttc tggaacggac
     9061 ggcctgttcg cggtcgattt ccgtttcctg aatctgtcgt tcagccagaa ttcgcgtctg
     9121 ctctgcttcc cgacgacgct cggcctcaaa agccgcaatt ttcgcattct gctcagcggt
     9181 acgggtttta acctgctgct cctgctcaag cgtcataaac gcttcttgct gttcaatctc
     9241 cagcttccgc gaaagcgcat cacggttttt ctcacgcacc gcaacttcta catcctgttc
     9301 aacttcgtta cgttcgcgac gacggcgctc cgtctcctga gtcagtttgg ttaaaccttc
     9361 ggcgtcaaag gcattgttcg gattgaaatg ttctttcgag gtctggttaa agttggtaag
     9421 tgaaacgctt tccagctcca ggccattttt cgacagatct tcagctacgg tattttgtac
     9481 tccctgcacg aagttctcgc gggtatcctg taactcatgc atggtcattt gtgccgctgt
     9541 tgcacggagg gcatcgacga atttatcctc aaccagcata cgtaaatctt ctggtgatag
     9601 agtgcgttgc cccagcgtct gggccgcagt ggcgatgcct tctactgacg gttttacccg
     9661 tacaaagaaa gccactacaa catcaacacg catacgatct ttcgtaatca ggctatcaat
     9721 agtagaacgg ctgacttcca gcttcagggt attcatattg atgggaatta tttcatgaaa
     9781 gatcggcatc acgattgcgc caccgctcat caccactttt tgcccactta aaccagtacg
     9841 aacaaatgct tgctctgccg aagcgcgacg atagagcctg gcgaaaataa ttccaataat
     9901 aaacagaatg catacggcga taatcgcggt aaacatccag gagggcacag aattaacgat
     9961 atcatccatt attaaatttc cttttgatta ataaatcaca atatctggga ccagggattg
    10021 ttttccgcca aatatcgcgt tgcagaaagt cggcaaataa ttaacacttt gtctccttta
    10081 gtgaaagttt ttccttcttc aggctccagt agcaaataat gtatttggcc aaattgatcg
    10141 gtgagttttc cttcgcaggg gttacctgaa gtggcttgat ggccggtaat taatgccatg
    10201 ctaccaacat attcttcttc tgtaatagct gaactatggt cgcgtggaat ccagggtgca
    10261 acgactttac cggtataatg cactgcaata attgtaaaca gtaaactgac aggaaccacg
    10321 aacaggttgg ggagtggtga ttgccagaac gtaacgcagg catgttgcaa caggatgcca
    10381 ataagaccaa aaaaaccagc gagtaagcag aggacgacga gagctggtaa tcttccaata
    10441 ttgagataat gaagtgcctg gctaatatga cctgtggtta tggaatcata atggtcaaga
    10501 tgtgcatcaa gagcaccgga gagcatgtga ccgcagataa gtgctaaaat ttctaaaagg
    10561 ccgatcaaga gtacaaagga aatagcgaaa agataagggg tgttatagtc ggcgaataaa
    10621 atcatatgtc actccaatgt ccatttagtg acgcaatatt tactttggaa tagtttcctt
    10681 gcttacattt gtctttgtta ataataaca
//
LOCUS       DADTFG010000154        10620 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.155, whole genome shotgun sequence.
ACCESSION   DADTFG010000154 DADTFG010000000
VERSION     DADTFG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.155"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            160..1068
                     /locus_tag="J1138_003878"
     CDS             160..1068
                     /locus_tag="J1138_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBA8455615.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1288..3279)
                     /gene="tkt"
                     /locus_tag="J1138_003879"
     CDS             complement(1288..3279)
                     /gene="tkt"
                     /locus_tag="J1138_003879"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA8455616.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3557..4315
                     /locus_tag="J1138_003880"
     CDS             3557..4315
                     /locus_tag="J1138_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBA8455617.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYARRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIHDRMSADGVK"
     gene            complement(4519..5439)
                     /gene="speB"
                     /locus_tag="J1138_003881"
     CDS             complement(4519..5439)
                     /gene="speB"
                     /locus_tag="J1138_003881"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBA8455618.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5575..6306)
                     /locus_tag="J1138_003882"
     CDS             complement(5575..6306)
                     /locus_tag="J1138_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455619.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPTQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6452..8428)
                     /gene="speA"
                     /locus_tag="J1138_003883"
     CDS             complement(6452..8428)
                     /gene="speA"
                     /locus_tag="J1138_003883"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBA8455620.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8437..8568)
                     /gene="yqgB"
                     /locus_tag="J1138_003884"
     CDS             complement(8437..8568)
                     /gene="yqgB"
                     /locus_tag="J1138_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBA8455621.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8704..8919
                     /locus_tag="J1138_003885"
     CDS             8704..8919
                     /locus_tag="J1138_003885"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455622.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            9223..10377
                     /gene="metK"
                     /locus_tag="J1138_003886"
     CDS             9223..10377
                     /gene="metK"
                     /locus_tag="J1138_003886"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBA8455623.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2641 a         2836 c         2671 g         2472 t
ORIGIN      
        1 atacacatct ctattttctt attgaatgtt cgaaaacata cttattacgt ttattatttt
       61 ttcgctcatc ccctttataa attctgaatt atcggctgtt gtctgttttc tcatcacccc
      121 atgtgacata ttcctatact ttactcaagg aagattcata tgaaagcacc tgtttcatta
      181 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      241 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      301 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      361 gtagagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      421 cgcgaccagc aaccggcaca aatgacagta tcagtcagtt tccacattaa accttcagaa
      481 gcaggcgcgg tttataccac ctacaacacc attgaagcat taaaagatcg tctgattgtc
      541 cgtcagttgc caactcaact ggagaacgtt ttcggacaat ataccgccat cagcgccgta
      601 caggatcgta caaaactggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      661 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      721 tcgattgaag accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      781 acggagaaaa tccaggcgca aatagcggta actcaggccc aggcggaagc agacagtaaa
      841 ctagcggcag ccaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      901 actattcgtt taaaaagtgc ggctgaagca gaagctatcc gcctgcgcgg tgaagctctg
      961 cgggagaatc ccggacttgt tgccctcacc actgctgagc gttgggacgg taaactgccc
     1021 gataccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1081 tttcgcggcc cttgagactg atagcaaaga caaaactgcc tgatgcgcta cgcttatcag
     1141 gcctacatac ttcctgcaat atgttgaatt tgcaaaatct tgtaggacgg ataaggcgtt
     1201 cacgccgcat ccggcataaa caacgcgcac tttgccaaca atctgaaggg ccgcatttgc
     1261 ggcccttctc acaaagcatc ttaccgatta cagcagcgct tttgctttcg caacaacgtt
     1321 gtcaacggtg aagccgaact cttcaaacag ctgctctgcc ggagcagatt caccgaaggt
     1381 ggtcatgccg acgatagcac cgttcaggcc aacatacttg taccagtagt cagcaatacc
     1441 cgcttctacc gcaacgcgtg cagtaaccgc tttcggcagt acggattcac ggtaagccgc
     1501 atcctgcttg tcgaatgcgt cggtagacgg catggaaacc acgcgcgctt tcacgccttc
     1561 ggcagtcagt ttttcgtagg cggcaacagc cagttcaact tccgaaccgg tcgcgatgaa
     1621 gatcagttcc ggctgaccgg cacagtcttt cagcacataa ccaccgcgcg cgatgtttgc
     1681 cagctgctct tcagtacgct cctgctgcgc caggttctga cgggagagga taagcgcagt
     1741 cgggccgtcc tgacgctcaa cgccgtattt ccacgctact gcggattcaa cctggtcaca
     1801 cggacgccat gtagacatgt tcggggtcac gcgcagagaa gcgatctgtt ctaccggctg
     1861 gtgagtcggg ccatcttcgc ccagaccgat ggagtcgtgg gtgtaaacca tcacctgacg
     1921 ctgtttcatc agcgcagcca tacgtacggc attacgtgcg tattccacga acatcaggaa
     1981 ggttgaggta tatggcagga aaccaccgtg cagggagata ccgttggcaa tcgcagtcat
     2041 accgaattca cgtacaccgt agtggatgta gttacccgca gcatcttcgt tgattgcttt
     2101 agaaccagac cacagggtca ggttagacgg cgccaggtca gcggagccgc cgaggaattc
     2161 cggcaacagc ggcccgaacg cttcgatagc attctgagac gctttacggc tggcgatctt
     2221 cgccggatta gcctgaagtt tagcgatgaa ctctttcgct ttggcgtcga agtcagacgg
     2281 catttcacct ttcatacggc gggtaaattc agcggcttcc tgcggataag ctttcgcgta
     2341 agcagcgaat ttctcgttcc atgcggactc tttcgcctgg cctgcttctt tcgcatccca
     2401 ctgagcatag atttcagacg ggatttcgaa cggcgcgtat ttccagccca gctgttcgcg
     2461 ggtcagggca atttcagcgt cgcccagcgg cgcaccgtgg gagtcgtggg taccggcttt
     2521 gttcggggaa ccgaaaccga tgatggtttt gcacatcagc agggacggtt tgtcagtcac
     2581 tgcgcgcgct tcttctactg cgcgtttgat ggatgccgcg tcatgaccgt cgatgtcgcg
     2641 aataacgtgc cagccgtaag cttcgaaacg cattgcggtg tcgtcggtaa accagccttc
     2701 aacgtgacca tcgatagaaa taccgttgtc atcgtagaac gcaatcagtt tacccagctt
     2761 cagcgtaccc gccagagagc aaacttcgtg ggagatgcct tccatcatgc agccgtcgcc
     2821 catgaaggcg taggtgtagt ggtcgacgat gtcgtggccc ggacggttaa actgcgccgc
     2881 cagcgttttt tctgcaatcg ccataccgac tgcgttggca ataccctgac ccagcggacc
     2941 ggtggtggtt tctacacctg cggtgtaacc cacttccggg tggcccggag ttttagagtg
     3001 cagctgacgg aagtttttca gttcttccat cggcagatcg taaccggtga ggtgcagcag
     3061 gctgtagatc agcatggagc cgtggccgtt ggacagcacg aagcggtcac ggtcagccca
     3121 ggacggattc tgcgggttgt gtttcaggaa atcacgccac aggacttcgg caatgtcagc
     3181 catacccata ggggcacccg ggtgaccgga tttggctttc tgtactgcgt ccatgctcag
     3241 cgcacgaata gcattggcaa gctctttacg tgaggacatt ttgactccag atcggatgat
     3301 gaagggcaca cccttaacga cttgacgaca gcgcgttttg ggctacgccg gaaaatttgc
     3361 caacaattta ccgcaagccg cgcgtcatgt acatggaaca tccttttgcc gcttcagaaa
     3421 tctctggatc atgctcgcat gttgcgcaat ccactcgccc gtccgctgcg cttttcctta
     3481 tactgagact gagcgtcgat tcacctgcaa acggcgcatt tttagaataa tcctgacctt
     3541 gtgcggaaga gaaaacatga aaattcgcgc cttattggta gcaatgagcg tggctacggt
     3601 gctgaccggt tgccagaata tggactccag tggactgctc tcatcaggag cggaagcttt
     3661 tcaggcttac agtttgagtg atgcgcaggt gaaaaaactg agcgatcagg catgccagga
     3721 gatggacagc aaagcgacga ttgcgccagc caatagcgaa tatgctagac gtctgacgac
     3781 aatttctcgc gcgttaggcg ataacatcaa cggtcagccg gtgaattaca aagtgtatat
     3841 ggcgaaggat gtgaacgcct ttgcaatggc taacggctgt atccgcgtct atagcgggct
     3901 gatggatatg atgacggata acgaagtcga agcggtgctc ggtcacgaaa tggggcacgt
     3961 ggcattaggc catgtgaaga aaggaatgca ggtagcactt ggtacaaacg caattcgcgt
     4021 tgcggcggcc tctgcaggcg gaattgtcgg cagtttgtca caatcacaac tgggagacct
     4081 gggcgaaaag ctagtcaatt cgcaatttac ccaacgtcag gaatcagagg ccgatgatta
     4141 ctcctacgac ttgctgcgcc aacgcggtat cagcccggca ggtcttgcca ccagctttga
     4201 aaaactggcg aaactggaag aaggccgcca aagctcaatg tttgacgacc atcctgcatc
     4261 cgccgaacgc gcccagcata ttcacgatcg tatgagcgcg gatggagtta agtaaagcca
     4321 ggtagtgccg aacgtaggtc ggataaggcg ttcacgccgc atctgacaag aatggcacat
     4381 ttgttactcc gtgcgcattg ccggatgcga tgctggcgta tcttatccgg cctacgggtg
     4441 ccgagtgtag gtcggataag gcgttcacgc cgcatccgac attaatggca catcacccgc
     4501 gcgcctctta tctggcgctt actcgccctt tttcgccgcc tggatataca gcatctccag
     4561 cgccagcgtc gccgcagcca gagcggtgat ttccgactga tcgtatgccg gagccacctc
     4621 cactacgtcc atcccaacga tgttgagatc tttcaagccg cgtaccagtt taatggcgcg
     4681 atcggaggtc aggccgccaa ttaccggcgt accggtgcct ggtgcaaaag caggatccag
     4741 gcagtcgata tcaaaggtca ggtaaaccgg catatcaccc acaatctgtt tcacctgggc
     4801 gataatgtca tccacgctgc gatcgttcac ctggcaggcg tccagcacgg taaagccgtt
     4861 gtctttatca aactcggtac gaataccaat ctgcacggaa tgattcgggt cgatcagacc
     4921 ttctttcggc gcggtataga acatggtgcc gtggtcaaat tcacaaccgt tagcgtaggt
     4981 atcggtgtgg gcatcaaagt gtaccaacgc cattttgccg aaatgcttcg catgagcgcg
     5041 cagcagcggc agcgtaacaa agtggtcacc accgaaggag agcatacgct taccggcagc
     5101 cagcagcttc tcggcgtgcg cctgcagctt ctcgctcatc tcacgggcat ctccgaaggc
     5161 atataccaga tcgccgcagt ccacgacgtt caggcgctca cgcatgtcga aattccacgg
     5221 gaagcgattg tgttcccagg ccagattcgt cgaaacctga cggatcgctg ccggaccgtg
     5281 gcgaccaccc gcacgaccag aagtggccat atcgaacggc acgccagtaa tcacccagtc
     5341 agcatcgctg tcatacggct ggaagttcat cggcaggcgt aaaaaaccaa aggcgttgga
     5401 aaccagtgag ttatcgtatt gatgacctaa ggtgctcatg gatggactcc tattacaaag
     5461 tcgatataaa aaaacccctc cgcgtcgtta ggcccgacga ggaagggtta gatttgcgaa
     5521 acacaagttt gtgctaatta tcgccctaaa tgggcaaggc atcaagcaag gttactattt
     5581 ccgacacggt ggcggcgcat agccattgtt atctgccgga aaggcttgtc cagagcacgg
     5641 cggcagcgcg ttttgcgtcg gaccttgtaa ttcaaaggcg gcaaagccga ttaccccggt
     5701 attacgcaca tcgccttgcg ccgaattggc tgcgtaggaa tcagcaacgt tggcgaactg
     5761 aaaagccgct tcggtatgtt tatctttgcg gaatccttta atcgctaacg aatctccggc
     5821 attcacaata tagccattgt tattcagcga accttgcttg ccgttcagca catccaggcc
     5881 atcaaccgta gcaacgatct cgtagttggt gttacgactg taattacgga catataattg
     5941 ataattcatt cctactctgg catgaacaat atagcgtggt tcattatttg acgaatcata
     6001 aacccgcgta ataggtagag aattaaaatt accatcgcga atagcatatt caagttcgtt
     6061 aatacgaatg ctataaacgc ggtcgtagcc cgaaggaaca gtagtcgaat aactaataac
     6121 gatcatctct gccggatcgc gccagactcg ttcagcagat acgccctgaa ctgatgagtg
     6181 tacatcccca ccccaggcgg tgccgacatt gcctgatgca ctgctattat actgcgcatt
     6241 actggaacaa ccggccagca gcacggctaa tgctatcaac gcgctacgta ctttccattt
     6301 tttcataacc gttccctctg catttaatta atgcagcgat tgtagggaga ttatttaata
     6361 agaaaagcag ctaaattatt tttaagaata tataaaaaga aagcgggccg atttacgtaa
     6421 ataaatcgac ccgccagcgg aataatctta attattcatc ttcaagatag gtataaccgt
     6481 acaaacctgc ctcgaactct tcaaggaact gttgttgcag ttcagcatca agatcggttt
     6541 tcttcacttg atcacggaac tgggttaaca acgttttcgg atcgagctgt acatattgca
     6601 gcatatccgc cacggtatcg ccttcgtcag acagttctac ttctacgctg ccgtcaggga
     6661 agacgaacac gtcaaccgct tcggtatcac cgaacaggtt gtgcatgttg ccgaggatct
     6721 cctgatatgc gccgaccatg aagaaaccga gcatcggcgg attctctgga tcgtactccg
     6781 gcattggcat tgtcgtggca ataccatcac catcgatgta gtgatcaata gcaccgtcag
     6841 aatcacaggt aatatccagc agcacagcgc ggcgttccgg cacttgatcc agcccttcca
     6901 gcggcagaac cgggaacaac tggtcgatcc cccacgcatc cggcatcgac tggaacagcg
     6961 agaagttgac gtacattttg tccgccatac gttcctgcag ctcgtcgata atcggacgat
     7021 gagcacggtt ttgcggatcc agctgctttt gcacttcatg gcacatgctc aaataaagct
     7081 gctcagccca tgcgcgttct tgcaggctaa aggtgccgga agagtagccg atatgaatgt
     7141 cgtgcagatc catctggctg tcgtgtaacc attcacgcag agaacggcga gttcccggtt
     7201 cgtgcatttc ctgccaggtt tcccacatgc tttgcagggc gcgcggtgcg tcttctgcag
     7261 gcgcggtcgg caccgtgtat tcgttacgtt ccacgccgat gatattcgac accagcacgg
     7321 tgtgatgcgc agtcaccgca cgacccgatt cggtgattac cgtcggatgt ggcagaccat
     7381 tttcttcaca cgcatcacca atcgcccaga tgatgttgtt ggcgtattca ttgaggccgt
     7441 agtttacgga gcagtcagac tgcgaacggg tgccttcata gtccacgccc agaccgccgc
     7501 cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag ttccacatag aaacgcgcgg
     7561 attcacgaac gcctgtcgcg atatcgcgaa tattcgccat ctgcgaaccg agatggaagt
     7621 gcagcagttg cagactgtcg agacgcccgg cttcacgcag cgtttcaacc agttgcagta
     7681 cctgagtcgc cgccaggcca aacttcgatt tttccccgcc ggaggactgc catttacccg
     7741 agccttgcga cgccagacgt gcacgcacgc ccagacgagg aacgacattc agacgttctg
     7801 cttcatccag cacaatggca atttctgaca tcttctcaat gaccagatag accttgtggc
     7861 ccatcttctc gccaattaat gccaggcgga tatattcgcg gtccttataa ccgttgcaga
     7921 cgatgacgct acgggtcatg ccagcatgtg ccagcaccgc catcaactcg gctttggaac
     7981 cggcttccag gcccagcggt tcgcccgaat gaatcaggga ctcaatcacg cggcggtgct
     8041 ggttaacttt gatcggataa acgaggaagt aatcgccgtt atagccgtag gattcccgcg
     8101 ctcgtttgaa cgcggcgtta atggaacgca aacggtgctg caggatctgt gggaaacaga
     8161 acagtgcagg cagacgctga ccctgtgctt cgcgagtttt cactaactgc gcgagatcga
     8221 cgcgagcttc cgggacgtcc gggtccgggc acacgctgat gtggcccagc tcgttaacgt
     8281 catagtagtt attgccccac caggcaatat tgtaagtacg cagcatcttg ctggcttcct
     8341 gggagctcat tgcaacctcc tgcatggagc gtagtacacc gtgttcgccc gctgacgaag
     8401 gcaaacccat agacatgtcg tcagacatag cgaacctcaa attattttat taagtgtaaa
     8461 acagttaacg actatcgcag cctggaactg cgataacaac ccataagcac atggattttc
     8521 cagcagtgaa tgctgacgct ccaactgcgc aaccggtttc tttttcataa cattattaag
     8581 cacataaccg aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc ttgtttttac
     8641 aagagcgccc ttgttcagtc ctcagtagct gtaaccagct cttgaatcct gagaagcgcc
     8701 gagatgggta taacatcggc aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc
     8761 ttcaccagaa cggtgagaca ggttaagcag cagacaacgg ttcattattt cgtatcacct
     8821 ccacggccgc ctgttaagac gaacccacaa gccaaaactc tgttttcaac ccggctggaa
     8881 gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac
     8941 cccacaatgg cagaaaattg caaaagataa atacgcagaa tgccagcatt gtcaggaaaa
     9001 atttccagcc acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg aaattgggtg
     9061 aagaactgac ctaaaatagc catccagatg ttaatccatc cataccgatt aacactcaga
     9121 ctgccagtgt ttttaacctg cagagtcgtg gtaggatccg ctaccacaga aaatccacac
     9181 aacagtttga gctaaccaaa ttctctttag gtgatattaa atatggcaaa acaccttttt
     9241 acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat ctctgatgcc
     9301 gttttagacg cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta
     9361 aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa
     9421 gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtac attccgacat gggctttgac
     9481 gctaactcct gtgcggttct gagcgccatc ggtaaacagt ctcctgacat caaccagggc
     9541 gttgaccgtg ccgatccgct ggaacagggc gcgggcgacc agggtctgat gtttggctac
     9601 gcaactaatg aaaccgacgt gctgatgcca gcacctatca cttatgcaca ccgtctggta
     9661 cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa
     9721 agccaggtga ctttccagta tgacgacggc aaaatcgttg gcattgatgc tgtcgtgctt
     9781 tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag
     9841 atcatcaagc cgattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac
     9901 ccgaccggtc gtttcgttat cggtggcccg atgggtgact gcggtctgac tggtcgtaaa
     9961 atcatcgttg atacctacgg cggcatggcg cgtcacggtg gcggtgcatt ctctggtaaa
    10021 gacccatcaa aagtggaccg ttccgcagcc tacgcagcac gttatgtcgc gaaaaacatt
    10081 gttgctgctg gcctggccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct
    10141 gaaccgactt ccatcatggt agaaactttc ggtactgaga aagtgccttc tgaacaactg
    10201 actctgctgg tacgtgaatt cttcgacctg cgcccatacg gtctgattca gatgctggat
    10261 ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc
    10321 ccgtgggaaa aaaccgacaa agcgcagctg ctgcgtgatg ctgccggtct gaagtaatct
    10381 ttcttcacct gctttcaaag gccagcctcg cgctggcctt tttcttttac ctcaccgcgg
    10441 tggacaggca ttcacgccgc atccggcaaa aaaaccgccc gcacaataac atcattcttc
    10501 ctcaacacgt tttaccgcaa attatcatca ctactgaaac cgattacacc aaccacgcca
    10561 gccaaatatt tgtaatattt tcatattatt attcagcctt cacagttgtt acattttttt
//
LOCUS       DADTFG010000155        10615 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.156, whole genome shotgun sequence.
ACCESSION   DADTFG010000155 DADTFG010000000
VERSION     DADTFG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.156"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            347..1156
                     /gene="pduF"
                     /locus_tag="J1138_003887"
     CDS             347..1156
                     /gene="pduF"
                     /locus_tag="J1138_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HBA8455624.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
     gene            1359..2273
                     /gene="pocR"
                     /locus_tag="J1138_003888"
     CDS             1359..2273
                     /gene="pocR"
                     /locus_tag="J1138_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HBA8455625.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPGPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSDNAAMETV"
     regulatory      2365..2540
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2758..3195
                     /locus_tag="J1138_003889"
     CDS             2758..3195
                     /locus_tag="J1138_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBA8455626.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PFDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLNGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            3329..3874
                     /gene="cobU"
                     /locus_tag="J1138_003890"
     CDS             3329..3874
                     /gene="cobU"
                     /locus_tag="J1138_003890"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBA8455627.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3871..4614
                     /gene="cobS"
                     /locus_tag="J1138_003891"
     CDS             3871..4614
                     /gene="cobS"
                     /locus_tag="J1138_003891"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBA8455628.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            4626..5705
                     /gene="cobT"
                     /locus_tag="J1138_003892"
     CDS             4626..5705
                     /gene="cobT"
                     /locus_tag="J1138_003892"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBA8455629.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            5767..6693
                     /gene="ldtA"
                     /locus_tag="J1138_003893"
     CDS             5767..6693
                     /gene="ldtA"
                     /locus_tag="J1138_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA8455630.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPVSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVNINRNGF"
     gene            6867..7832
                     /locus_tag="J1138_003894"
     CDS             6867..7832
                     /locus_tag="J1138_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455631.1"
                     /translation="MTKQNKNSRWQGYDPRYIYSLVVRRYFADMKTKIEIAEELGVSR
                     FKVARLIDEAIEQDYVRFIFPKQQAMDEEIANNLRKKFHLEDAIVLSVAESWTTQEEL
                     NHKLGEVTAEYLMQSLHEGMKVGIAWGRVLSSTVSKLSKLPPLDVVQLSGVHPGIEFS
                     QGPIDLIHKIAAISQGKAHPMYVPMWVDDEELAARLAGDPAVLDTQQYYSQLDVVITG
                     IGDWKSGSSSLCKIFPDTWCEALFQQDIAADVCISLVSREGKILHSPIERLGFGISTD
                     QLQKAKKVIGVAGGEEKYEGILASLKSGLLNVLITDFDTAIKLLD"
     gene            complement(7884..8342)
                     /locus_tag="J1138_003895"
     CDS             complement(7884..8342)
                     /locus_tag="J1138_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA8455632.1"
                     /translation="MKFEILVNDVDNSISDYQTAITFAGTQLLEKGYITAEYIDACLE
                     REKSYPTGLMMANGQGIAIPHADYTLVNVNSISIVRFANDVTFGQMEDADLTVDCSIM
                     FNLAFATSDQHMSILRRLFTLFQDNAFVETCRNLPATRVAKYVEDTLAAS"
     gene            complement(8353..8637)
                     /locus_tag="J1138_003896"
     CDS             complement(8353..8637)
                     /locus_tag="J1138_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA8455633.1"
                     /translation="MKKILVVCGNGIASSSIMVSVLQDFLKEEKIEAQVDKSSLMACT
                     SDTFNSYDLVVSSTKLDNPAITTRVIVGAGLLTGLGEDEIFAAVKEEMLK"
     gene            complement(8661..10031)
                     /locus_tag="J1138_003897"
     CDS             complement(8661..10031)
                     /locus_tag="J1138_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA8455634.1"
                     /translation="MELITQFINDLGNFIFIPVIFLVLMKILGRPLSECISSAIKVGI
                     GFIALTMTIKLMLEKMQPAVTGLAEATGSSLSAIDVGGAATAVMGFGSSMGAIIIPLC
                     VAVNIAMLVARLTDCVNVDVFNLHQNASMGAIVGVYSGSFLYGALTAALFHVWALIAA
                     DLGAKNNEKFFNLPKGVAISHPVANTYLLFAYPFNWIYDRIPGFRNLNVTAETIQKRF
                     GILGDPTMVGFIIGILLGFCGYGWKSPYHTIIASLQLGMYLAAVMLLLPRMTSIMMEG
                     LVPLSNVARKKLVKRFPDRQITVGMDTALIVGHPSVIAPALLLIPVIVILAVILPGNR
                     VMPLGDLSQFVFFIACMVPVFNGNIIRTWVTSIILFGGGLYIASWMAPATNEVFQKFG
                     TNPDASVMYSSLNPSANPFTGLFAALSHVGIIGYVMAGILLLSIGYLIKQKSRRQIET
                     DLEKAL"
     gene            complement(10035..10571)
                     /locus_tag="J1138_003898"
     CDS             complement(10035..10571)
                     /locus_tag="J1138_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBA8455635.1"
                     /translation="MKTKIAIASDDVGFARKEEIKKYLIEEKNMEIVYDPVTCKEQGV
                     NNFARLADEMAGIIQRDECRLGIYICGTGIGFTCQINKHWGIRAVAVTNPYSAKRARL
                     SNNAQVIGLGCRVNDLEYSKMIVDAWLDNPFDFTTARENSKKNLLEAERNDNALLVKP
                     ADVRWNMGFRPDDEKSEG"
BASE COUNT         2718 a         2259 c         2692 g         2946 t
ORIGIN      
        1 aatgtgaatt aattgcgttg gctagtgagt ttaatcgcga gtaatatttc gttgagtttg
       61 gttcgaagga aggaactgtg atatttgttt caattttatt atgtcatgat ggcaattttt
      121 tattgtaaaa gtttggtgga aattattact ttcaacactg attaacgctg tgttgcatga
      181 ttcggcaatg acaataattt gttatgttag cacgattttt acacgagtct gtcatgcatg
      241 ttggcaatat tttctgcaga gcaatttttt atagttgcgt tcggtataca caacaatcag
      301 cgcatagttg gctgataaag aaaaactccg taaagaaggt gtcactatga atgattcact
      361 caaggcgcaa tgcgttgccg agtttttggg caccggactg ttcctctttt ttggcattgg
      421 ttgtttgtgc gccctgaagg ttgccggggc aagtctggga ctgtgggaaa tttgtatcat
      481 ttggggatta gggatttcgc ttgctgttta ccttacggca ggtatttccg gcgctcatct
      541 caacccggca gttaccattg ctctgtggct gtttgcctct ttcccagccc gtaaagtgct
      601 gccattttgt gtggcgcaat tagcgggtgc ctttggtggt gcggttctgg catattctct
      661 ctacagcagt ttgtttaccg attttgaatc tgctcacaat atagtgcgtg gtagcgcaga
      721 aagtttacaa ctcgccagta tcttcagtac ttatccagca gcggcgatta atgtatggca
      781 agctgcgttg gtcgaagtgg tcataacgtc catgttaatg gggttgatta tggcgttgac
      841 cgatgacggt aatggcgtac ccaaaggacc gcttgcgcct ttactcattg gtattctggt
      901 tgctgttatt ggtgcttcta ccggaccatt aaccggtttt gccatgaatc cagcgcgtga
      961 ttttggacct aaaattttca cctggtttgc gggttggggg gaaattgcga tgactggtgg
     1021 acgcaatatt ccttatttca ttgtacccat tattgcaccg atcattgggg catgtgttgg
     1081 tgcggcgatt tatcgctatc tcattgcaaa gaatttacct gtcaatagtg taacgcctaa
     1141 agaaatcagc gaataacgaa ataatctggc agttataaac gtttattttt ttactgtttg
     1201 tgatctgctt tccgaactta ttaaaaagtc tcccgcttca cggtggcttt tgttattgtc
     1261 ctttgtgctt ctcgtcagta aatcatcaag gatgatgtga agattaaatg taaaacaatt
     1321 gttttgctat aacaataaat taactgggag gttttatcat gatttctgcg gcgacactta
     1381 attcagaact cattaataaa atcgcgcagg attttgcgca ggcgaccagc cttgcggttg
     1441 tcgtggtcaa tattcacggc gatgaaattt ccgagctgtt taacttcact ccattttgtc
     1501 agatgatgcg gcaacaccct cagctgagca ctcgatgtcg catgagcgat cgttgtgggg
     1561 gtctggaagc ctctaaatcc gatcagcctt gtatttatcg ctgtcatgcc ggattaaccg
     1621 atttctctat tccactggta atcgccggac atttagttgg ttttgtattg tgcgggcaag
     1681 ttcgtctgcg taatgatgat gtcgatttgg tcgatattct gaatgtcgat gattgctggc
     1741 aggcagatcc cgaattactt aatgaatttc gcaaagtccc tgagatggat tactcaagag
     1801 ttatggcctc tgcggatttg ttgaagttga ttgtagaaaa ttgcctaaaa aaacaactca
     1861 attttgtggt tattaaagag aacaaatctc agtcagattc ggtgcgccag actcgtgcgc
     1921 ctggtcccca cgacaataaa atgaaaaaag ctttacgtta tattgatgcg catttatcag
     1981 acgatttgcg tctggaagat gtcgcctcgc acgtttatct aagcccttat tatttcagta
     2041 agttatttaa gaaataccat ggaatcgggt ttaatgcgtg ggtaaaccaa caacggatgg
     2101 ccagcgctcg tgagttactg tgccatagtg actggagcat cgccagcatt gcgcgtaatt
     2161 taggtttttc gcaaacaagt tatttttgca aagttttccg tcagacttat caggttacgc
     2221 cgcaggtgta tcgacagcaa gtgagtgata atgcggcgat ggaaacggta tagagtaaat
     2281 gccccaaaaa aagaggacgg tttaaataaa ctctgcaatg aataattaac gttatttccc
     2341 tgttatggga aaagtgaaat cagctcataa catacgctga caggtgtggc acatttgtgc
     2401 ctgggaagag ggtgaaaatc cctcgcagcc cccgctgctg taatgctgac aaacccgaaa
     2461 atgccactga tcgcgagatt gggaaggtcg ggagaggatg acgctaagcc agaagaccag
     2521 ccagtcagtc aataccaaca acttcggtgg gaagtgggtt gcccggaagc gtccggtaga
     2581 gtacaggcgc gtctttatac ccctaccacc ctgaaaggat cagggtatgg cggcatcatt
     2641 tagttctttc atcgcagtgc cgacaggcac cagggctgca cttaaggtgt ggcaatcttg
     2701 tttcatggct tatcaaccag accgccatta caggaaatta agtggagggc gaggggcatg
     2761 aaaacgataa aacccgaatc gatagctctt ttttgcctct tatctggaga tgtggatctg
     2821 gcgaaacatc tggcggaaat attgccaata acctgttttg ttggtgagga ctggcagcaa
     2881 gatttccagc cttttgacgg tgggcctgtg caatcgttat cagatgcgta tagcgattat
     2941 tcagtgctgc tattgatagc agctgaaagc gagatatccg taccagaatg gctcgctcat
     3001 tctgagggaa cgacggtaat tttgattgca gaacacaatg cccgtttgct gaatggtccg
     3061 gctggtgggg ctgtagcatt aatccattat ctgaatgaga tctgtcatgc cagagaaacc
     3121 gctgtcaatg acgcattttg tcggacgtta acacgaggaa gtctggctat tccgcaatgc
     3181 catcttcttt cgtaatggat tgagaatgct ggatttttaa ttatattacg cgcagtatta
     3241 atcgcgtaaa tgttttgtgg tcagcaacag tgggggatcc attgttctgt tcaatgaata
     3301 ccatcataca tttgaatcag gagtcggtat gatgattttg gtgacgggag gcgcacggag
     3361 cgggaagagt cgccacgcag aggtgcttat tggggactct tcacaggttc tgtatatcgc
     3421 tacctcgcaa atccttgatg atgagatggc tgcacggata gaacatcatc ggcaaagccg
     3481 cccggagcac tggcgcacag tggagcgctg gcaacatctt gatgaattaa ttcatgcaga
     3541 cattaacccg aatgaggctg tgttgctgga atgcgttacc acaatggtga ctaatctgtt
     3601 gtttgattat ggcggcgata aagaccctga tgaatgggat tatcaggcga tggaacaggc
     3661 gattaatgct gagattcagt cgttgattgc tgcctgccaa cgttgccccg caaaggttgt
     3721 attagtgact aacgaagtgg gaatggggat tgtgccggag agtcgtctgg cacgacattt
     3781 tcgtgatatt gccgggcggg taaatcagca gttggccgct gcggcaaatg aagtatggct
     3841 ggtggtttcg ggtattggag taaaaatcaa atgagtaaat tattttgggc gatgctctca
     3901 tttattacgc gtctgcctgt accgcgtcgc tggtcccagg gactggattt cgagcattat
     3961 tctcgcggta ttattacttt tcctttgatt ggattattgc ttggcgcgat tagcgggctg
     4021 gtcttcatgg tgctgcaggc atggtgtggc gtaccactgg cggcactgtt tagcgtactt
     4081 gtgctggcgc tgatgaccgg gggattccat ctggacggcc tcgctgatac ctgcgatggc
     4141 gtattttctg cacgtagccg tgatcgcatg ttggaaatca tgcgtgatag ccgtttaggc
     4201 acccacggcg gtctggcatt gatttttgtg gtactggcaa agattctggt gttaagcgag
     4261 ttggctctgc gtggcgaacc gatccttgca tcgctggcgg cagcatgtgc ggtcagccgc
     4321 gggactgccg cattattgat gtatcgtcat cgttatgcgc gggaagaagg gcttggcaat
     4381 gtgttcattg gcaaaattga tgggcgacaa acctgcgtca cactcggctt agccgctatt
     4441 tttgctgccg tattattgcc gggtatgcat ggtgtggctg ctatggtggt gacgatggtg
     4501 gcgattttca tcctcggtca gcttctcaaa cgtacgctgg gcgggcaaac gggcgatacg
     4561 ctgggagcag ccatagagtt tggtgaactg gttttcctgc tggcactgct ataaagagac
     4621 gtgacatgca aatacttgcc gatttactga atacgatccc agccatcaat tctgccgcta
     4681 tgtcgcgtgc acaacggcat gttgacgggt tactcaaacc tgttggtagc ctgggaaagc
     4741 tggaggcgct tgccatacaa ctggcaggaa tgccggggtt gaatggcata ccgcatgtgg
     4801 gcaaaaaagc agtactggtt atgtgtgccg atcacggcgt ctgggaggaa ggggtcgcta
     4861 tttctccaaa agaagtgaca gccatacagg ctgaaaatat gacccgtgga acaaccggcg
     4921 tgtgtgtgct ggcagcacaa gcgggcgcta acgtccacgt aattgatgtt ggtattgata
     4981 ctgctgagcc tatccccggg cttatcaaca tgcgtgtcgc acgaggtagc ggcaatattg
     5041 cttcagctcc ggcaatgagt cgccgtcagg ctgaaaagtt gcttttggac gtcatatgtt
     5101 atacgcggga gctggcaaaa aacggtgtca cgctgtttgg tgtaggtgaa ctggggatgg
     5161 caaacacgac accggcagcg gcaatagtca gcacaatcac tggccgggat cctgaagaag
     5221 tggttgggat tggcgcaaac ctgccgacag ataaactggc taataaaatt gatgttgtgc
     5281 gtcgggcgat tacgttgaat caaccaaatc ctcaggatgg tgttgatgtc ctggcaaaag
     5341 tgggtggatt tgatttggtc ggaatagctg gagtgatgtt aggtgctgct tcctgcggtt
     5401 tacccgtgtt gctggatgga tttctttctt atgctgctgc gctcgcagcc tgccagatgt
     5461 ctcctgcaat caaaccgtat ctcattcctt ctcacttgtc ggcagaaaaa ggcgcgcgta
     5521 tagcgctctc gcatttgggg ctggagcctt atctcaatat ggatatgcgt ttaggtgagg
     5581 ggagtggtgc agctctggcg atgtccatca tcgaagctgc ttgtgcgata tacaacaaca
     5641 tgggcgaact tgctgccagt aatattgttc taccggggaa tacgacttct gatttgaaca
     5701 gctaaaaacg aaaagtctga taaaagctat acttatcgga tagtattcgc gacttaggaa
     5761 agggacatga tgcgtcgtgt aaatattctt tgctcatttg ctctgctttt tgccagccaa
     5821 aatagcctgg cggtaactta tccattacct ccagagggta gccgtttagt ggggcagtcg
     5881 cttactgtaa ctgttcctga tcacaatact cagccgctgg agacttttgc cgcacaatac
     5941 gggcaagggt taagtaatat gctggaagcg aatccgggcg ctgatgtttt tttgccgaag
     6001 cctggctcac aactcaccat tccgcagcaa ctgattttgc ccgccactgt tcgtaaaggg
     6061 attgtggtta acgtcgctga aatgcgtctt tattactatc cgccagtcag taatacagtg
     6121 gaagtctttc ctattggtat cggccaggct gggcgagaaa ccccgcgtaa ctgggtgact
     6181 accgttgaac gtaaacaaga agcaccaacc tggacgccaa cgccgaacac tcggcgcgaa
     6241 tatgcgaaac gaggggagag tttgcccgca tttgttcctg cggggcccga taaccccatg
     6301 gggctgtacg cgatttatat tggcaggttg tatgccatcc acggtaccaa tgccaatttt
     6361 ggtattgggc tccgggtaag tcagggctgt attcgtctgc gcaatgacga tatcaaatat
     6421 ctgtttgata atgttcctgt aggcactcgt gtacaaatta ttgatcagcc agtgaaatac
     6481 acaacggaac cggatggttc aaagtggctg gaagttcatg agccgctgtc gcgcaatcgt
     6541 gcagaatatg agtctgaccg aaaagtgcca ttgccggtaa ccccatcttt gcgggcgttt
     6601 atcaacgggc aagaagttga tgtaaatcgc gcaaatgctg cgttgcaacg tcgatcggga
     6661 atgcctgtga atatcaaccg taacggattt tgacattcaa aacgtctggt ttgtcggtct
     6721 gtgtaagcat tccgtcatcc agaacaggtt gtttcgaatc atacaaatga taattttctt
     6781 ctcatatgcg catctcttat gagcataaac gttatactct tctgatttat acatgtttgc
     6841 ggatgacatg atcaatggaa aagagaatga cgaaacagaa taaaaacagc cgttggcagg
     6901 gttacgatcc tcgttacata tactcgctcg tggtcagacg ctattttgcg gatatgaaaa
     6961 ccaagattga gattgctgag gagcttggcg tttcccgctt taaagttgcc agattgatcg
     7021 atgaggcgat cgaacaggat tatgtgaggt ttatcttccc taagcagcaa gcgatggatg
     7081 aagaaatcgc caataatcta cggaaaaaat ttcatctgga agatgcaatt gttctttcag
     7141 ttgctgaatc atggacgacg caagaagaac tcaatcacaa attgggtgaa gtcaccgctg
     7201 aatatcttat gcaatctctt catgaaggta tgaaagtggg gatcgcctgg ggacgtgtat
     7261 tatcaagtac ggtcagtaag ttgagcaagc tgcctccttt agacgttgtg cagttatctg
     7321 gcgtacatcc ggggatagag tttagtcaag ggccaataga tcttattcat aagatcgctg
     7381 ccatttcgca gggaaaagcg cacccaatgt acgtgccgat gtgggtcgat gacgaagagc
     7441 ttgctgccag actggcaggt gatccagcgg tattagatac acagcaatat tactcgcagt
     7501 tggatgtggt tatcaccggg ataggtgact ggaaatcagg ttcttcaagc ttgtgtaaaa
     7561 tatttccgga tacctggtgc gaagctttgt ttcaacaaga tatcgctgcg gatgtgtgta
     7621 tctcgttggt cagcagggaa gggaagattc ttcatagccc tattgaacgt ctgggatttg
     7681 gcatttcgac ggatcaacta caaaaagcca aaaaagtgat tggtgtcgct ggaggggaag
     7741 aaaaatatga aggcattctt gcttcgctga aatctggact tttaaacgtc ttaattactg
     7801 attttgatac ggctattaaa cttctggatt aatagccgta tcagtgaaga aagggcagta
     7861 ccgctgattc tgccctttta atttcaggat gctgcgagag tatcctctac atatttagct
     7921 acgcgcgttg caggtaaatt gcggcaagtc tcaacaaagg cgttatcctg aaacagtgtg
     7981 aataaacgac gcaatatcga catatgttga tcactggtgg caaatgccag gttaaacata
     8041 atgctacaat caactgttaa atcagcatct tccatttgcc cgaaagtgac atcattggcg
     8101 aaacgaacaa tactgatact gtttacattt accagcgtgt aatcggcatg gggaatggcg
     8161 ataccctgac cgtttgccat cattagccct gtcggatagc ttttttcacg ttccaggcag
     8221 gcgtcaatgt attctgcagt gatgtagcct ttttccaata attgcgtacc agcaaaggtg
     8281 atcgccgttt gatagtcact gatgctgtta tcaacatcat tcaccagaat ttcaaatttc
     8341 attgcacacc tgttatttca acatctcttc tttaactgca gcgaaaatct catcttcgcc
     8401 cagaccagtc agtaatccag cgccaacgat gacacgagta gtaatggccg ggttatcgag
     8461 tttggtcgat gaaaccacca ggtcatagct gttgaaggta tcgctggtgc atgccattaa
     8521 cgaagattta tcgacttgcg cttcaatttt ctcttctttc aggaagtcct gcagtacaga
     8581 gaccatgatg gaagatgagg caattccgtt tccgcaaact actaagattt ttttcatttg
     8641 attattcctc tgtaaaagat ttaaagcgct ttttctaaat ccgtttcaat ctggcgacgt
     8701 gatttttgtt taattaagta tccaatagat aacaaaagga tacctgccat cacatagcca
     8761 atgattccaa catggctcag ggcggcaaac agaccagtaa atggattcgc agacgggtta
     8821 agcgaagagt acatcacgct ggcatccggg tttgtaccaa acttctggaa gacttcgttg
     8881 gtagccggtg ccatccacga tgcaatatac agaccaccac cgaacaaaat gatcgaggtc
     8941 acccaggtgc gaataatgtt gccattgaaa acaggcacca tgcaggcaat gaaaaacaca
     9001 aactgagaga ggtcacccag tggcataacg cggttgccgg gcaagatcac tgcgaggatc
     9061 acaatcaccg ggatcagtaa taatgccggg gcgataactg acggatgacc cacaatcagg
     9121 gcagtgtcca ttccaacagt gatttgacga tccgggaaac gtttgaccag ttttttgcgt
     9181 gccacgttgg aaagcggaac aagcccttcc atcatgatag aggtcatacg tggcaacaga
     9241 agcatgactg cagccagata catccctaac tgtaggctgg caattatggt gtggtagggg
     9301 gatttccagc cataaccaca aaagcccaac aaaataccaa taataaaacc aaccatagtt
     9361 ggatcgccca gaatgccaaa ccgtttttga atagtttcgg cggtcacatt cagattacgg
     9421 aagcctggga tgcgatcata aatccagttg aatggataag cgaaaagtaa gtaggtattg
     9481 gcaaccgggt gagagatcgc aacacctttt ggcaggttaa agaatttttc gttattttta
     9541 gcaccgagat cggcagcgat cagcgcccag acatggaata gcgcggcggt caatgcgcca
     9601 tacaggaagc taccagaata gacgccaaca attgccccca ttgacgcatt ttgatgaagg
     9661 ttgaaaacat caacgttaac acagtcagtc aggcgggcga ccagcattgc aatatttacc
     9721 gcaacacaga ggggaataat gatagcgccc atgctggagc caaatcccat cactgcagtc
     9781 gctgcgccac caacatcgat ggcactgagc gaggaacctg ttgcttctgc taatccggtg
     9841 actgccggtt gcattttttc cagcatcagt ttgatggtca tggttagcgc aatgaaacca
     9901 atgcctactt tgatggcaga cgagatacat tcagaaagag gacggccaag tatcttcatc
     9961 agtaccagga agatgaccgg gataaatata aaatttccca gatcgtttat aaattgcgtg
    10021 atcagttcca tacattaacc ctcagatttt tcatcatctg gtcggaaacc catattccag
    10081 cgtacatcag cgggcttaac cagcaatgca ttatcgttac gttcagcttc cagtaagttc
    10141 tttttagaat tttctctggc ggtggtgaag tcgaatgggt tatccaacca tgcatcgaca
    10201 atcattttgc tgtattcgag atcgttgacc cggcaaccca gtccaataac ttgtgcgtta
    10261 ttgctgagtc gcgcccgctt tgcggagtag gggttagtaa ccgcaacggc tcttatgccc
    10321 cagtgtttgt tgatctgaca agtaaagcca atgccagtcc cacaaatgta aattcccaga
    10381 cggcactcat cgcgttgaat aatgcctgcc atttcgtcgg ccagacgcgc gaagttattg
    10441 acaccttgtt ccttacatgt gacgggatcg tacacgatct ccatgttttt ctcttctatg
    10501 aggtattttt ttatctcctc tttacgtgca aaacctacat catcgcttgc gatagcaatc
    10561 ttcgttttca tttcattttg cctgtcgtta agtaactaaa gccaagtatg ctgtt
//
LOCUS       DADTFG010000156        10574 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.157, whole genome shotgun sequence.
ACCESSION   DADTFG010000156 DADTFG010000000
VERSION     DADTFG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.157"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(49..957)
                     /gene="melR"
                     /locus_tag="J1138_003899"
     CDS             complement(49..957)
                     /gene="melR"
                     /locus_tag="J1138_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBA8455636.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSQNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1240..2595
                     /gene="melA"
                     /locus_tag="J1138_003900"
     CDS             1240..2595
                     /gene="melA"
                     /locus_tag="J1138_003900"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBA8455637.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(2592..3242)
                     /locus_tag="J1138_003901"
     CDS             complement(2592..3242)
                     /locus_tag="J1138_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBA8455638.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGAPWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(3365..5011)
                     /locus_tag="J1138_003902"
     CDS             complement(3365..5011)
                     /locus_tag="J1138_003902"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBA8455639.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(5089..6429)
                     /locus_tag="J1138_003903"
     CDS             complement(5089..6429)
                     /locus_tag="J1138_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBA8455640.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(7000..7719)
                     /gene="dcuR"
                     /locus_tag="J1138_003904"
     CDS             complement(7000..7719)
                     /gene="dcuR"
                     /locus_tag="J1138_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBA8455641.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(7716..9347)
                     /gene="dcuS"
                     /locus_tag="J1138_003905"
     CDS             complement(7716..9347)
                     /gene="dcuS"
                     /locus_tag="J1138_003905"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBA8455642.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MHSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            9528..9758
                     /gene="yjdI"
                     /locus_tag="J1138_003906"
     CDS             9528..9758
                     /gene="yjdI"
                     /locus_tag="J1138_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBA8455643.1"
                     /translation="MDQALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDMCPSGALKYRHK"
     gene            9770..10042
                     /locus_tag="J1138_003907"
     CDS             9770..10042
                     /locus_tag="J1138_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA8455644.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            10271..10567
                     /gene="ghoS"
                     /locus_tag="J1138_003908"
     CDS             10271..10567
                     /gene="ghoS"
                     /locus_tag="J1138_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBA8455645.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
BASE COUNT         2614 a         2544 c         2741 g         2675 t
ORIGIN      
        1 ttattatcga cggctaaact atttttttgg ctgatactga tatcgtcttt agccgggaaa
       61 cgtctggcgg cgctgttggc taagtttgcg gtattgttgc ggcgacatgc cgacatattt
      121 gccgaacgtg ctgtaaaaac ggctactcga acgaaagcct gccgtcaggg caatatcgag
      181 aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg ttgatgcgca tcgcggtaat
      241 gtactgtttc atcgtcaatt gcatgacccg ctggaatatc cccattgcat agttggcgtt
      301 aagtttgacg tgctcagcca catcgttgat ggtcagcgcc tgatcatagt tttcggcaat
      361 aaagcccagc atctggctga cataaaattg cgcatggcgc gagacgctgt ttttgtgtgt
      421 gcgcgaggtt ttattgacca gaatcggttc ccagccagag aggctaaatc gcttgagcat
      481 caggccaatt tcatcaatgg cgagctggcg aatttgctcg ttctgactgt ttaattcctg
      541 ctgccagcgg cgcacttcaa acgggctaag ttgctgtgtc gccagtgatt tgatcaccat
      601 gccgtgagtg acgtggttaa tcaggtcttt atccagcggc caggagagaa acagatgcat
      661 cggcagatta aaaatcgcca tgctctgaca ggttccggta tctgttagtt ggtgcggtgt
      721 acaggcccag aacagcgtga tatgaccctg attgatattc actttttcat tgttgatcag
      781 gtattccaca tcgccatcga aaggcacatt cacttcgacc tgaccatgcc agtggctggt
      841 gggcatgata tgcggtgcgc gaaactcaat ttccatccgc tggtattctg aatacagcga
      901 cagtgggcta cgggtctgct tttcgtcgct gctgcacata aacgtatctg tattcatgga
      961 tgactctctt tcctggaata tcagaattat ggcaggagtg agggaggatg actgcgagtg
     1021 ggagcacggt tttcaccctc ttcccacagc ggcgagggga gcgtccgagc atcatgaggc
     1081 cgaaaactct gcttttcagg taacttattc ccataaactc agatttactg ctgcttcacg
     1141 caggatctga gtttatggga atgctcaacc tggaagacgg aggttttctg cagattcgcc
     1201 tgccatgatg aagttattca agcaagccag gagatctgca tgatgtctgc acccaaaatt
     1261 acatttatcg gcgctggttc gacgattttc gttaaaaata ttcttggtga tgtgttccat
     1321 cgcgaggcgc tgaaaacggc gcatattgcc ctgatggaca ttgatcccac ccgcctggaa
     1381 gagtcgcaca ttgtggtgcg taagctgatg gattcagcag gggccagcgg caaaatcacc
     1441 tgccacaccc aacagaaaga agccttacag gatgctgatt ttgtcgtggt ggcatttcag
     1501 attggcggtt atgaaccttg cacggtgact gatttcgagg tctgtaagcg gcatggtctg
     1561 gaacaaacca ttgccgatac gctggggccg ggcggcatta tgcgtgcgct acgtaccatt
     1621 ccgcacctgt ggcagatttg cgaggacatg acggaagtct gccccgatgc caccatgctc
     1681 aactacgtta acccaatggc gatgaatacc tgggcgatgt atgcccgcta tccgcatatc
     1741 aagcaggtag ggctgtgcca ttcggtgcag ggaacggcgg aagagctggc gcgtgacctc
     1801 aatatcgatc cggctacgct gcgttaccgt tgcgcaggta tcaaccatat ggcgttttac
     1861 ctggaactgg agcgtaaaac cgcagacggt agctacgtga atctctatcc tgaattgctg
     1921 gcggcttatg aagcagggca ggcaccgaag ccaaatattc atggtaatac tcgctgccag
     1981 aatattgtgc gttacgaaat gttcaaaaag ctgggctact tcgtcacaga atcgtcagaa
     2041 cattttgctg agtacacacc gtggtttatt aagccaggtc gtgaggattt gattgagcgt
     2101 tataaagtac cgctggatga gtacccgaaa cgctgcgtcg agcagctggc gaactggcat
     2161 aaagagctgg aggagtataa aaacgcctcc cggattgata ttaaaccgtc acgggaatat
     2221 gccagcacaa tcatgaacgc tatctggact ggcgagccga gtgtgattta tggcaatgtc
     2281 cgtaacgatg gcttgattga taacctgcca caaggatgtt gcgtggaagt ggcctgtctg
     2341 gttgatgcta atggcattca gccgaccaaa gccggtacgc taccttcgca tctggccgcc
     2401 ctgatgcaaa ccaacatcaa cgtacagacg ctgctgacag aagctattct tacggaaaat
     2461 cgcgaccgtg tttaccacgc cgcgatgatg gacccgcata ctgccgccgt gctgggcatt
     2521 gacgaaatat atgctcttgt tgacgacctg attgccgccc acggcgactg gctgccaggc
     2581 tggttgcacc gttaaggggt aatcgcttcc ggcgctctgg tgattaagcc aaagcgccgt
     2641 agctggcggc aatgaaagcg ggcgaggaat atcaccgtcg ttaatgcgcc cagtagtgcg
     2701 cagaacatat cggattgcgt atcccacggg gcgccttgag taccgagaaa atcatctgcg
     2761 ccctggccca ttgccagcgc tgcccaccac tcgattaact catacatcgc gcttatcgcc
     2821 agcgcgacac aacagaccag aaacgccacc attttacgcc cgcgaacgta catcccgcgc
     2881 acgaggatct ctcgtgccac cagtgcaggc accagcccct ggaaaaaatg ccccagtttg
     2941 tcatacggat tacggctcaa ccccagccat tcctgcacct caaaaccaac ggggactttc
     3001 gcgtaggtgt attgcccgcc gaccatcagg atgatggcgt gaaagaaaat gagcgtatag
     3061 agcagcggcg ttaacgggta acgtttagcg gtggcaagca gtagcggcac gacaataatc
     3121 accggtgtca cttccatcag ccaggtgagt ttgtcatggg ccgaaatgcc ggtataaatc
     3181 aggattaacg ttagcgccag tgcgccggtg ttaagaatta acggcttgag tgtgcgggtc
     3241 atggtgagtt cgcaagtcag ggaaaatact gactattgac cagtaagcgt gaaattacaa
     3301 tcgcaaaaga ggtggcccag ggggatcacc tggcagcatg ctgccaggcg ctgggccgaa
     3361 gaggttactt agtgcagttc gcgcactgtt tgttgacgat ctgctggaag aagtcgttac
     3421 ctttgtcatc gaccaggata aacgccggga aatcttctac ttcgattttc cagatagctt
     3481 ccatacccag ctccggataa gcgacgcatt ccagatgctt gatgctctgc tgcgccagta
     3541 ccgccgccgg accgccgatg ctaccgaggt agaagccgcc gtgtttatgg cacgcgtcgg
     3601 taacctgctg gctgcggtta cctttcgcca gcatgatcat gctgccgccg tgggattgca
     3661 gcagatccac gtaggagtcc atacgacctg cggtggttgg gccaagtgaa cctgatggat
     3721 aaccggcagg cgttttcgcc ggacccgcgt agtagatcgg gtgatctttg atgtactgcg
     3781 gcagttcttt accggcgtca atcagctctt tcagcttggc gtgtgcaata tcgcgaccca
     3841 cgataatggt gccggtgagc gacaagcggg tggataccgg gtattgcgaa agctgggcaa
     3901 ggatctcttt catcgggcgg ttaaggtcga ctttcactgc ttcgccttca cctgcctgac
     3961 gcagttcttc tggaatgtac tggcctggat tgtgttccag tttttcgatc cagatacctt
     4021 cgcggttgat tttcgcttta atgttacggt cagcggagca ggagactccc atgcctaccg
     4081 ggcaggatgc gccgtgacgt ggcagacgga taacgcggat gtcgtgcgcg aagtatttac
     4141 cgccaaactg cgcgccaaga ccgagtttct gggcctcttc cagcagttcc tgttccagct
     4201 ggatatcgcg gaacgcctga ccatgttcgt tcccttccgt cggcagttca tcgtaatagt
     4261 gagcgcttgc taacttgacg gttttcaggt tggtttccgc agacgtaccg ccaatcacaa
     4321 acgcgatatg gtacggcggg caggctgcag taccgagggt acgcattttc tcgacgagga
     4381 agtttttcag tttgccgggc gtcagcaggg ctttggtttc ctggtagaga tacgttttgt
     4441 tggcagagcc gccgccttta gcgacacaga ggaatttgta ctcatcgcca tctaccgcgt
     4501 acaggtcgat ttgcgcaggc aggttcgtgc cggtgttgac ctctttatac atgtccagcg
     4561 gcgcgttctg tgaatagcgc aggttatctt cgatataggt gttatagacg cctttcgaca
     4621 gcgcttcttc atcaccgcca ccggtccaca cgcgctgacc ttttttaccg acgatgatcg
     4681 cggtgccggt atcctggcag gtcggcagca cgcctttggc ggcgatttcg gagtttctta
     4741 agaattgcag cgccacgtac ttgtcgtttt cgctggcttc tggatcgtga agaatagccg
     4801 caacctgttt ctggtgtgct gggcggagca tgaaagaagc gtcgtgaaag gcttgctgcg
     4861 ccagcagggt cagggcttct ggttccactt tcaggatggt ttcgccgtcg aagtcggcaa
     4921 cgctaacgta atcggaagtg agtagatagt attcggtatt gtctttcccc atcgggaaag
     4981 gtgcctggta gataaagggt ttgtttgaca tagcttccag cctgtaactc tgtatttgtt
     5041 attcgaaaat ggcgtgccgc gtagttacgg gcggcacgca aagtgcattt ataagaaccc
     5101 gtacatcgcg gcgaagatcc agccgaagac gcacgatacg ctcacaccaa tcaaccccgg
     5161 cagaataaag ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg aacggtcaaa
     5221 ctgaatcgct gccagatcgc tcggataagt cggcaggatg taataaccgt agcaagccgg
     5281 tgctgaagcc acgatgtatg ccggatcaac gccgatcgcc agcgcgaccg gaacaatcgc
     5341 cgccagcgct gcagcctgag agtttacaaa cttggaaacc agcagcagaa caatggcata
     5401 ggcccacgga tactctttca ccatttcacc cagtacgccc tgaatttcag acatatgcgc
     5461 accgaacatg gtttcagcca tccatgcgat accgtacacc gccacgatgg cgatcatacc
     5521 ggaacggaag acttcgtttt ttgagataga cgcgggattg gttttggtca ggataataat
     5581 cagcgccccg gtcagcagca taaacatctg aataaccagt accatcgaca gcggtttgcc
     5641 gccgaaggat ggacgcaggt ccgaatcagc accaagaagg gcgactacag cgattgcccc
     5701 gaggaagatc cacattgcca gccagttgct tttcggcagt tttttatcca gcagcgtcgc
     5761 ggtatcaccg taaacatact cacggttttc cggtacggag atgaatttct ggaactcttc
     5821 gtctttatcc agatctttac cgcggaacca gctgaagata ccgatcgcca ggataccgat
     5881 taacgtcgat ggaatggtga ttgccagcag gtcgaggaac tcaagatggc gaccatcaaa
     5941 ggtgacatta cccagcatcg caaccaaaga tacgaccgca accgacaccg gactggcgat
     6001 aatccccatc tgtgcaccga tagaacttgc cgccatcgga cgttccggac ggatgttgtt
     6061 cttaatagca acgtcgtaga tgatcggcag aatggtgtaa accacatgac ccgtaccgca
     6121 aagaatggtc agcgtacagg tcacaaacgg cgcgacaatt gagacatatt tcgggttgcg
     6181 acgcagcagc ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag cttgcaaggt
     6241 cgccgatgcc gccaccaccg caatgataac cagcatgaca tcaaccggtg gtttacctgg
     6301 ctgaaggtgg aagacgaaga ccagaatgac cagaccgata ccgcctaata aacccagcgc
     6361 gatacccccc tttctggcac cataaaacag acatatcagt attatgataa gttggatagt
     6421 aaataacatg tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa tagatcacag
     6481 tcacgttctg ttttgtatga actgttttca gagcctgact aaatatccgt ttggtcgtta
     6541 ccggcttgag caaatacctc acagtgaata ttggctgatt aaagcagcgg caaatttatg
     6601 cactgtgata agcggctttt tcagagagaa agactctctg gcgatgggtt tatagtttgt
     6661 acagcgcaga tgttaatgtt ttgtttgctg ttatcttatt gatatttatc ggtctgtttt
     6721 ttgttctcag ggcaggtatc attaggttta tagattaatc tgatctaccc atttgtgggt
     6781 aaaaatacac ataatgtggg tgacataata gttaattaac ttttgttagc gttttgaaat
     6841 taaaaacacc gttcatctga agagatatta atttttagcg atgatggagg gataattata
     6901 tttgatctgg cacaagtttt actgatgaat aatgtaactt gtgccaggaa tattttgcat
     6961 tacggtaata attattactt acagataacc agcgatccgt tattggcaat attgtttcag
     7021 cagtgagtag tgttcagcct gaatacggta acggtaaacc ggacgccccg taacgccata
     7081 atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt tacggcagga
     7141 aacacgcgag atgttaacct cgttggccag ctcgtcggtt gaaaactcat agtcctgatg
     7201 cgcgtcaatc cactggcaca gcgtgcgtaa cgtctgcggc gttaagcctt ttggcaagcg
     7261 acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct cggcctggtc
     7321 ataatactga tgtttttcca gcgccatttt cttttgccgc cagccggtga gcgcctcttc
     7381 aaagcgggaa gcctggaagg gtttgatcag gtaatccaca acaccgtaat gcagcgaatc
     7441 tttgatggtt gctgcatcgg cagcggagga gatgacaatc acatcacttt tgcaacgcgc
     7501 gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga tatctagcaa
     7561 tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct ccagcgtcga
     7621 agccgttcca cagcattgaa agcccgggat ttgtgccacg tatcggcgat tcagctccgc
     7681 gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct ctccccgtcc
     7741 cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac ggcgatgctg
     7801 ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg ctcgcttcct
     7861 tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat ccccggtcca
     7921 tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac gctaatttcg
     7981 ccaccgggtt ccggccctaa tgcctccagc gcgttttcta tcagatttcc caacgtggta
     8041 atcagcgtcg cgacctgatc ctcactaccg ctgtctggca gctggctttc actgtttaaa
     8101 atcagcgtat ggcctaaatc ggtcgcacgg ttaatcttgc tgattaaaaa accagcgata
     8161 accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt attggctgtt
     8221 ttgagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc gagaatcaca
     8281 tgcaatttat tcataaattc gtgggatcgt tcacgaagtg cgtcagcata gttgaccaga
     8341 ccgtcgagtc gctgcatcag tttacgtact tcagttttgt ccctgaaggt tgaaatggca
     8401 ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag tcggtcctta
     8461 atcgtaatct cttcgtcgcg gcgtggggtt ccgtcgcgta acacttccga gacatctacc
     8521 acctgtgacc acgagtggct tagcgtcgac agtttctcat cgtcctgcga cttacggtaa
     8581 ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc gtccacggca
     8641 acgacgcctt ctttgatgga ctgcaacatg gcctggcgtt gctcaaacag tgtggagatt
     8701 tcgtagggtt ccaggccgaa aaggattttt ttcagtacgt taaccagaat gcatgtgccg
     8761 atcagtccga ccagcatgcc aaatagtacc gaccagataa tgctccagcg actgtcattg
     8821 atctgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat ttgtttatga
     8881 ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc gcgattgata
     8941 gcgacatttt cttcgccatt cagcgcttta aggatgtcat cacctttaaa tggctgacca
     9001 atacgctggg cttcaggatg cgagtagcga agactgtgca tatcggtaac gacaataaac
     9061 agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggattccact ctcctgcggt
     9121 tttttctgca agccctgacg gatttccggc gagtcggcga gggtacgcgc tactgccaga
     9181 gccttgttag ctagcccgtc tcgcgtcata tcactgattt gcgagaagta aatcagatgc
     9241 accaccaata gcaccgagaa cagtaccgcg ctgaccatta agatcacagt ggtactcaat
     9301 ttcatcggac gtttgcgtaa catacggtag ggcaatgaat gtctcatcag cttccttgtg
     9361 tgacaaattt cttaagcatt atctctgatg aggcgggtaa ttcaaaggga gtaagaatga
     9421 ttggctatat aggggaagag actctggcaa cggaaactgc cagtgctgta tgaagattcc
     9481 ggggctatgc ttatagtgaa aaccatactg atgagagagg gaaggtcatg gatcaggcgc
     9541 tactggacgg gggttatcgc tgttataccg gcgaaaatat cgatgtctat tttaacactg
     9601 cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt aatctcaaac
     9661 gaaagccgtg gatcatgccg gatgaagtcg acgtcaccac tgtggtgaaa gtgattgata
     9721 tgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa tggaaatacg
     9781 cgaaggccac aataaatttt acattaatga cgaacaaggc aagcaaatcg ctgaaattgt
     9841 ctttgtgccg accggagaga atttagcgat tatcgaacat accgatgtcg atgaaagcct
     9901 gaaagggcaa gggattggta aacagctggt tgcgaaagtc gtggaaaaaa tgcgtcggga
     9961 aaaacgaaaa attatcccac tatgcccatt tgcgaaacac gaatttgata aaacacggga
    10021 gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt catgctctcc
    10081 gcggtgtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat ggtcagagag
    10141 agcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaaaagaa tatctcctat
    10201 atgagaatca tcattcgggg ttaataagtt ttgcgtcccc agagcgttca atattgatag
    10261 gagtcatatt atggaaggta aaaacaagtt caatacttat gttgtttctt ttgattatcc
    10321 atcatcttat tcctcagtgt tcttaaggtt aagatcattg atgtatgata tgaatttctc
    10381 ctctatcgtg gctgatgaat atgggatacc acgacaactg aatgaaaact ccttcgcaat
    10441 aacgacatcg ttagccgcaa gtgaaatcga agatttaatc aggctcaaat gcttagactt
    10501 accggatatt gattttgacc tcagcattat gacagttgat gactatttcc gtcagtttta
    10561 caagtagcca ggaa
//
LOCUS       DADTFG010000157        10572 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.158, whole genome shotgun sequence.
ACCESSION   DADTFG010000157 DADTFG010000000
VERSION     DADTFG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.158"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            164..2305
                     /locus_tag="J1138_003909"
     CDS             164..2305
                     /locus_tag="J1138_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HBA8455646.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQENYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2369..4261)
                     /gene="parE"
                     /locus_tag="J1138_003910"
     CDS             complement(2369..4261)
                     /gene="parE"
                     /locus_tag="J1138_003910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBA8455647.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4290..4871)
                     /gene="yqiA"
                     /locus_tag="J1138_003911"
     CDS             complement(4290..4871)
                     /gene="yqiA"
                     /locus_tag="J1138_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBA8455648.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4871..5698)
                     /gene="cpdA"
                     /locus_tag="J1138_003912"
     CDS             complement(4871..5698)
                     /gene="cpdA"
                     /locus_tag="J1138_003912"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBA8455649.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPGRHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(5723..6145)
                     /locus_tag="J1138_003913"
     CDS             complement(5723..6145)
                     /locus_tag="J1138_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBA8455650.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6146..6775)
                     /gene="nudF"
                     /locus_tag="J1138_003914"
     CDS             complement(6146..6775)
                     /gene="nudF"
                     /locus_tag="J1138_003914"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBA8455651.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6980..8461
                     /gene="tolC"
                     /locus_tag="J1138_003915"
     CDS             6980..8461
                     /gene="tolC"
                     /locus_tag="J1138_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBA8455652.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYTPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8609..9280
                     /locus_tag="J1138_003916"
     CDS             8609..9280
                     /locus_tag="J1138_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBA8455653.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDEIV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9286..10446
                     /locus_tag="J1138_003917"
     CDS             9286..10446
                     /locus_tag="J1138_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBA8455654.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2682 a         2801 c         2591 g         2498 t
ORIGIN      
        1 tagcaatttt ttgttatttc tcatttacta ctacattact gcgtgattga atgattctca
       61 attacatatc cgatggcgaa ataacaaacg gatagtgctt agtgagcaat aagaaacaat
      121 tttcactcgc gacaatccgc gagtttttgg gataacagac taaatggcta tgttcacacc
      181 ttcattctca ggactcaaag gtcgggcgct cttttcactg ctttttgcgg caccgatgat
      241 tcatgcaaca gactctgtaa cgaccaaaga tggcgaaaca atcactgtta cagcagatgc
      301 aaataccgca actgaggcaa ccgatggtta tcaacctctg agcacctcca cggcgacatt
      361 aaccgatatg ccgatgctgg atatcccgca ggtggtcaat acggttagcg atcaggttct
      421 ggaaaaccag aatgcgacaa cgctggatga ggcgctttat aacgtcagta acgtggtaca
      481 gaccaataca ttaggcggga ctcaggatgc ttttgtacgc cgtgggtttg gcgcaaaccg
      541 ggatggctcc atcatgacca acggtctgcg aaccgtactt cctcgtagtt tcaacgccgc
      601 aacagagcgt gtggaagtgc taaaaggccc ggcctccacg ctgtatggca ttctcgatcc
      661 tggcggactg attaacgtcg tgaccaagcg cccggaaaaa acattccatg gttcggtttc
      721 agccacctcc tccagttttg gtggcggcac tgggcaactt gatatcacag gtcccattga
      781 aggcactcag ctggcgtatc gccttaccgg ggaagtgcag gatgaagatt actggcgaaa
      841 cttcggtaaa gagcgcagta catttattgc cccgtcactc acctggtttg gtgataatgc
      901 aacagtaacc atgctctatt cccatcggga ctataaaact ccattcgatc gtggaacgat
      961 tttcgacctt acgacgaaac agcccgtaaa cgttgatcga aaaatacgtt ttgacgaacc
     1021 gtttaatatt acagatggtc agtccgatct ggcgcaactc aacgcagaat atcatctcaa
     1081 tagccagtgg acagcgcgct ttgattacag ctacagccag gataaataca gcgataatca
     1141 ggcgcgtgtt accgcgtatg atgcaacgac aggaacactg acacggcgtg ttgatgcaac
     1201 tcagggatct acccagcgta tgcatgctac tcgtgcggat ctgcaaggga atgttgatat
     1261 tgccggattc tataatgaga ttctgggtgg ggtgtcatat gaatattatg atcttctgcg
     1321 tacagatatg attcgctgta aaaaagctaa agatttcaat atatacaacc ctgtttatgg
     1381 taataccagc aaatgtacaa cggtttcggc gtcggacagc gatcagacga tcaaacagga
     1441 gaactactca gcttatgcac aggacgcgct ctatctgacc gataactgga ttgccgtcgc
     1501 cgggatccgc tatcagtatt acacgcaata tgcgggtaaa ggccgtcctt ttaatgtcaa
     1561 tactgacagc cgcgatgaac aatggacgcc caaactgggg ttagtctaca aactgacgcc
     1621 atcggtatcc ttatttgcca attattcgca aacatttatg ccgcagtcgt caattgccag
     1681 ctacattggc gatcttccac cagaatcatc taatgcttac gaagtcgggg caaaattcga
     1741 gctattcgat ggtatcaccg cagatattgc gctgtttgat atccataaac gtaatgtgtt
     1801 gtataccgaa agtattggtg atgaaaccat cgccaaaacg gcaggccgcg ttcgttcaag
     1861 aggggtagaa gtcgaccttg cgggagcatt aactgaaaac attaatatca ttgccagcta
     1921 cggctatacc gatgcaaagg ttctggaaga tcctgattat gcagggaaac cattgccgaa
     1981 tgttcctcgt cataccggtt cgctattcct gacctatgat attcataaca tgccaggcaa
     2041 taacacactg acgtttggcg gtggcggaca tggtgtaagc cgtcgttcgg caaccaatgg
     2101 ggctgactat tatctgccag gctattttgt tgccgatgcc ttcgccgcat acaaaatgaa
     2161 attgcagtat ccggtcacac tgcaattaaa cgtcaaaaac ctgtttgata aaacgtatta
     2221 cacctcttcc atcgccacaa ataatctggg caaccagatt ggcgatccgc gtgaagtgca
     2281 attcacggtg aaaatggaat tttgatcaaa aaagcccggt atacacttgt ataccgggct
     2341 atgttttatt cttctggcgg gaggattatc agacctcaat ctccgccatg tcgccttttt
     2401 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
     2461 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
     2521 gatcaagcgt ggtttcgcgc aattgcatcg ggttcatttc cccgagacct ttaaaacgct
     2581 ggacgtttgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
     2641 ccgtcagcgc gtaataaacc tctttcccga ggtcaatacg gtagagcggt ggcagtgcaa
     2701 cgtaaacgtg accgtgtttc accaatgcgc ggaagtgttt tacaaacaaa gcgcagagca
     2761 gcgtggcaat gtgcagacca tcggagtcag catccgccag gatacagatc ttgccgtaac
     2821 gaagctggct caaatcgtcg ctgtcaggat cgataccgat agctaccgaa atatcgtgca
     2881 cttcctgcga agccagcact tcgtcggaag agacttccca ggtattaagg atcttacctt
     2941 tcagtggcat gatcgcctga tattcgcgat cgcgcgcctg cttggcagat ccgcctgcgg
     3001 agtcaccttc cacaaggaat agttcggtac ggttaagatc ctgcgcggta cagtcagcca
     3061 atttaccagg caacgccggg ccgctggtta gctttttacg caccactttt ttagccgcac
     3121 gcatacgacg ctgggcgctg gaaatcgcca tctctgccaa cagctccgcc gcctgaacgt
     3181 tctggttcag ccacaggatg aaggcatctt tcaccacgcc cgaaacgaat gccgcacact
     3241 gacgcgaaga gagacgttct ttggtctgcc cggcaaactg cggatcctgc atttttactg
     3301 acagcacata ggcgcagcga tcccagatat cttccgccga cagctttaca ccgcgcggca
     3361 gaatgttgcg gtattcacag aactcacgca tcgcgtccag caggccctga cgcaggccat
     3421 taacatgggt accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt
     3481 caccgccttc cggcagccac agtagcgccc agtccaccgc ttcagtatcg ccagcgaaat
     3541 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
     3601 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaaag
     3661 tgatctcaac gcccgggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
     3721 cagaaaaacg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
     3781 tattgcgttt accgcaagtg ccgacaacct gtaaatcttg caccttttcg ccattttcaa
     3841 aggcgatgtt atagatctga ccatcgcggc gtacgttaac ttctacgcgc ttcgacaggg
     3901 cgttaactac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
     3961 agaatttacc gcccgcgtgc agacggcaaa gaatcagttc aaccgccggt accccctctt
     4021 ccgggtgaat atctaccggc atcccgcgcc catcgtcaat cacttctaac gactggtcgg
     4081 catgtaagat tacatccacg cgttttgcgt ggcccgccag tgcttcatcg acactgttat
     4141 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
     4201 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
     4261 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
     4321 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
     4381 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
     4441 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
     4501 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt ctcgttctga
     4561 ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggttgaccac cactgcgggc
     4621 agcataaaac attgcgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
     4681 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcagaa
     4741 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
     4801 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
     4861 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaagcgtgcg tccgccaggc
     4921 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
     4981 cgatggtgtc cagcgtaaag ttagaacagt gcggcttaaa ctgcacacag gtcgacggcg
     5041 ttgccagcaa gcggcgacca ttccagtcga gatccagctc ctgatgaatg tgaccgcaca
     5101 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
     5161 ggctgtgttg atcaagccaa ctacaacctg caggtagcgg atgatgatgc agcagcagca
     5221 acgtatggcg tcctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
     5281 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
     5341 caccaataaa cacgcgcttc gccggggaga tacccgcatc ctgtaacgca ctgtacatcg
     5401 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
     5461 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
     5521 caatcaggtc gaattcgtgc tggtgtggcc gaatcgcctc cagcaccgcc tggtagcttt
     5581 cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga gtgtcggtaa
     5641 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
     5701 ggtgtcctta ggtttcacga cgctaataaa ccggaatcgc catcgctcca tgtgctaaac
     5761 agtatcgcaa ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca
     5821 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
     5881 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
     5941 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
     6001 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
     6061 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
     6121 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
     6181 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
     6241 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
     6301 gaccgtgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
     6361 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
     6421 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttcc ccctcttcaa
     6481 tcatcccggc aaccatctcc agtagccagg gggtttcgct ggtgtcgtac gcggcaatcc
     6541 gaatctgctc aatcagcaca acttcatcac gtactgggtc aaagggtagc aagactgcgg
     6601 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
     6661 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
     6721 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
     6781 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
     6841 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
     6901 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
     6961 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctctc
     7021 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
     7081 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
     7141 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
     7201 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac
     7261 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagctgg
     7321 gattcaggac gtcacgtatc agaccgatca acaaaccttg atcctcaaca ccgcgaccgc
     7381 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcgc aaaaagaagc
     7441 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
     7501 cgacgtgcag aacgcccgcg cacagtacga taccgtgctg gcgaacgaag tgaccgcacg
     7561 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
     7621 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacacgct
     7681 gctgaaagaa gccgaaaaac gtaacctgtc gttgttacag gcgcgcttga gccaggacct
     7741 ggcgcgcgag caaattcgcc aggcgcagga cggtcactta ccgactctgg atttgacggc
     7801 ctctaccgga atttctgaca cctcttacag cggttcaaaa acccgtggtg ccgctggtac
     7861 ccagtatgac gatagcaata tgggccagaa caaagttggc ctgagcttct cgctgccgat
     7921 ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc
     7981 cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
     8041 caacattaat gcttctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
     8101 aagctcatta gacgcgatgg aagcgggtta ctcggttggt acgcgtacca ttgttgatgt
     8161 gttggatgcg accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa
     8221 ctacctgatt aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct
     8281 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcccc
     8341 gcaaacgccg gaacagaatg ctattgctga tggttatacg cctgatagcc cggcaccagt
     8401 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
     8461 acgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
     8521 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
     8581 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
     8641 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
     8701 ttttatgctg gcgggctgtg aaaagagtga tgaaatagtg tctctctatc aaaatgctga
     8761 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
     8821 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
     8881 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
     8941 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
     9001 gggacgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
     9061 cccggccagc ccggcttacg gtaaatatac cgacgcgacc ggtaaaaact atggcgcagc
     9121 ccagccaggc cgaaccatga ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac
     9181 tactactgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
     9241 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
     9301 attaccgagc gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc
     9361 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgctcaggtt
     9421 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
     9481 gtgatcgcca gcgatgagct gatgaccaaa ttccgcatac caaaacacac ctggagtttt
     9541 gtgcgccagt catggctgac gcaccagcca tcgctttatt cgcgtcttga tctggcatgg
     9601 gatggtactg gcgaacctaa acttctggaa aataacgccg atacgccaac gtcactatac
     9661 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
     9721 gagggcagcg accagtttaa cagtttgcaa gaaaagctga tcgatcgctt cgttgagctg
     9781 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
     9841 cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc
     9901 ctctacatcg acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
     9961 gtgatttcca acctgttcaa gctgtatccg tgggaattta tgttgcgtga gatgttctcc
    10021 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    10081 aacaaagcgc ttctaccgct actgtgggag atgttcccga atcacccgaa cctgctgccc
    10141 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    10201 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    10261 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    10321 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    10381 cgtgaagacc gtgcattgat cacccaggat atgtctcggt tttatccgca tatttttgtt
    10441 gagtaataca gcttaatatt gccccgataa gcggggcaat ctcttataat ccaccattca
    10501 taactcccat caatatataa acacatacat atccaacctt tatttaacat taatactcaa
    10561 tttaatgtta aa
//
LOCUS       DADTFG010000158        10477 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.159, whole genome shotgun sequence.
ACCESSION   DADTFG010000158 DADTFG010000000
VERSION     DADTFG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.159"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            102..2279
                     /gene="iroN"
                     /locus_tag="J1138_003918"
     CDS             102..2279
                     /gene="iroN"
                     /locus_tag="J1138_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HBA8455655.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKYSDDDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSEEGATRRANFSLSGPLAGDALTTRLYGN
                     LNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDRDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKERLNWNTNATWMITSEQKDTGNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(2324..3280)
                     /gene="iroE"
                     /locus_tag="J1138_003919"
     CDS             complement(2324..3280)
                     /gene="iroE"
                     /locus_tag="J1138_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HBA8455656.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSTTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(3365..4594)
                     /gene="iroD"
                     /locus_tag="J1138_003920"
     CDS             complement(3365..4594)
                     /gene="iroD"
                     /locus_tag="J1138_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HBA8455657.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            complement(4698..8483)
                     /gene="iroC"
                     /locus_tag="J1138_003921"
     CDS             complement(4698..8483)
                     /gene="iroC"
                     /locus_tag="J1138_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HBA8455658.1"
                     /translation="MIIICQPPSRSHASITMRDRSVLAFLRNDPASGSYFTDGGGLMP
                     ANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAALTPLLTRQAVNDALAGN
                     PARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRGRVFDSIQKLDGAGQDA
                     LRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVMLWMSPAMTLIVVCVLVC
                     LAITALRARRRVFAQTGMASDQLANLTEHIREVLAQISVVKSCVAEMRETHWLDRQSR
                     QIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHGRIDLGTFVAFASFLAM
                     LTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESINSQVVGLELENMSFDY
                     HHGDRHILSDISFSLRAGETVAVVGASGSGKSTLLMLLARFYDPCSGKIWLNTSEGRQ
                     NLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATADDIRRAAAAAGASDFIN
                     ALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDDTTSAVDAVTEAEINTA
                     LGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPAELEARCPAFRALMTGD
                     SDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPENAVQQALAGKGRKVTS
                     LLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGDMSTIGLCALLALCLVI
                     VGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEKHADSRLTRMTVDVDSL
                     ARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPVAALATMIYRRLSTPAY
                     AQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSERFRATRVRAQKYLAVY
                     FPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQFYGPVQQLSGIVDAWQ
                     QATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSYPDSHEPALNKLTLTIP
                     EGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDDASLTEYRRQIGLVDQD
                     VALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQGFRTPVNNGGADLSAG
                     QRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDVVHAGKHSALIVAHRLT
                     TAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSSTALHRQHNMKEETPG"
     gene            complement(8497..9612)
                     /gene="iroB"
                     /locus_tag="J1138_003922"
     CDS             complement(8497..9612)
                     /gene="iroB"
                     /locus_tag="J1138_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HBA8455659.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIRGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPDRKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            10150..10449
                     /locus_tag="J1138_003923"
     CDS             10150..10449
                     /locus_tag="J1138_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455660.1"
                     /translation="MKPTLLQTVENICLQILFVLFLFTGTLIDVEALVSCSSVAKNVQ
                     RTPASSVSSSVYSASVSRTAADIMLLSNNPLFAPGQVSFFGCSLAASGIHVTILE"
BASE COUNT         2484 a         2821 c         2816 g         2356 t
ORIGIN      
        1 tcattcttat ataaaaacaa taatggcagt aagaaatatc tggcaaggat gtgagcttaa
       61 cgatcaaaca ttttaagtta ggcatttatt agggaatagg aatgagaatt aacaaaatcc
      121 tctggtcgct aactgtgctc ctagttgggt tgaatagcca ggtatcagta gccaaatact
      181 ccgacgatga taatgacgag actctggtgg tggaagccac cgctgagcag gtattaaaac
      241 agcagccggg cgtgtcggtt attaccagcg aggatattaa aaagacccct ccggtaaacg
      301 acctttcaga tattattcgt aaaatgcctg gtgttaatct taccggcaat agcgcctcgg
      361 gcacacgcgg taataaccgc cagatcgata ttcgtggtat ggggccggaa aacaccttaa
      421 ttttaattga tggtgtaccg gtgacgtcac gtaactccgt gcgttatagc tggcgtgggg
      481 agcgtgatac ccgcggtgac accaactggg tgccaccgga acaggttgag cgtattgaag
      541 tgatccgcgg ccctgcggcg gcgcgctacg gttcgggggc cgccgggggg gtggtgaaca
      601 tcattaccaa acgtcccacc aacgactggc acggttcgct gtcgttatac accaaccagc
      661 cggaaagtag cgaagagggc gctacgcgtc gcgccaattt cagccttagt gggcctctgg
      721 ctggtgatgc tcttaccacg cgtttgtatg gtaacctgaa taaaacggat gctgacagtt
      781 gggatattaa ttctccggtc ggtacgaaaa acgcagccgg gcatgaaggg gtacgtaaca
      841 aagatattaa cggcgttgtc tcgtggaaat taaatccgca gcagattctc gatttcgaag
      901 ccggatatag ccgccagggg aatatctatg cgggcgatac gcagaacagt tcttccagtg
      961 cagttaccga aagcctggca aaatccggca aagagacgaa ccgcctgtac cgacagaatt
     1021 atggcattac gcataatggt atctgggact ggggacaaag tcgctttggt gtttattacg
     1081 agaaaaccaa taatacccgc atgaatgaag gattatccgg cggtggtgaa ggacgtattt
     1141 tagcgggtga aaagtttacg accaatcgcc tgagttcctg gcgaaccagc ggtgagctta
     1201 atattccttt gaatgtgatg gttgatcaaa cgctgaccgt tggtgcagag tggaaccgcg
     1261 ataagctcga tgatccttcc tctaccagcc tgacggtgaa tgacagagat atcagcggta
     1321 tttctggctc tgctgcggat cgcagcagta aaaatcattc tcaaatcagt gcgctgtata
     1381 ttgaagataa cattgagccg gttcctggca cgaatatcat tcccggcctg cgctttgatt
     1441 atctcagcga ctccggcggg aacttcagcc ccagtctgaa tctttcgcag gaattgggcg
     1501 attatttcaa agtcaaagca ggggttgccc gaacctttaa agccccaaac ctgtatcaat
     1561 ccagtgaagg ctatctgctc tactcgaaag gcaatggctg tccaaaagat attacatcag
     1621 gcgggtgcta cctgatcggt aataaagatc tcgatccgga aatcagcgtc aataaagaaa
     1681 ttggactgga gttcacctgg gaagattacc acgcaagtgt gacctacttc cgcaatgatt
     1741 accagaataa gatcgtggcc ggggataacg ttatcgggca aaccgcttca ggcgcatata
     1801 tcctcaagtg gcagaatggc gggaaagctc tggtggacgg tatcgaagcc agtatgtctt
     1861 tcccactggt gaaagagcgt ctgaactgga ataccaatgc cacatggatg atcacttcgg
     1921 agcaaaaaga caccggtaat cctctgtcgg tcatcccgaa atatactatc aataactcgc
     1981 ttaactggac catcacccag gcgttttctg ccagcgtcaa ctggacgtta tatggcagac
     2041 aaaaaccgcg tactcatgcg gaaacccgca gtgaagatac tggcggtcta tcaggtaaag
     2101 agctgggcgc ttattcactg gtggggacga acttcaatta cgatattaat aaaaatctgc
     2161 gtcttaatgt cggcgtcagt aatatcctca ataaacagat cttccgatct tctgaagggg
     2221 cgaataccta taacgagcca ggccgggctt actatgccgg agttaccgca tcattctgat
     2281 cgtagtgttt caggcgtact gcccgcgtaa aagcgggcag tgtttagtgg cttaactcat
     2341 gacaacctgc tgtgtaattt gcgttttcac cactgatatc taacagtgcc tggcgaaagg
     2401 aggcattgaa catcggcccg tgtcccaggt tggggaaatc ccaaaatacg gcattgacgc
     2461 ctttatcttt cagtatagtg agggtggtat gaattttcga cagcaccccg acagcatgcg
     2521 tttcccggtt atcaccctgt gtcgccgagc cttccattat cgccaggtgt ttggcgcaga
     2581 attgcagagg ctcaaccgcc gtaacgcggc ttagcaaagc atcataacct ctgcccaacg
     2641 acgggctggc gctgtagtac gaccggaagt aagaggagga cagccaggaa tccagcacga
     2701 agaggccgcc gtaggagtgc ccccataagc cgcggcgttg ccgatcgata ttcagtccct
     2761 gttccacttt tggggcaata cgcgtttcca gtaactggcg gaagttgttg ctgccaccac
     2821 tcttacggct aaaacgccct gagtggagat ctgtttttct gctttctgct gctggcgtat
     2881 agtcgtaagc cctgctgttg agatcgaaag ggaggttggt ctgatacccg acagccacga
     2941 tcactggcgg tgttttttct gacaattgtt tgagcagttc gtcatccagg cggtccataa
     3001 ctgcgttacc gtcaagcata tataaaatcg ggtaacccga tgccggtgcg gttgtattcg
     3061 gcacggccgt ccatacccga taatggcgtg tgccatcgac agagtcgaaa gaggtggtgc
     3121 tgaaatggta aaacacggag cctttatcgg ctatattcgg ccccagtggc cgcatatccg
     3181 gcttcgcata aacttgcgca ctacagataa gggtgaggca agaaagatga aagaaaatcg
     3241 ctttatgcgg gcgtgttgag cgatactcgc gggcatacat ctgatgatta ttccttttcc
     3301 gcaggtgaca atttgattta ttcaggaagt ctgtaccgtt tcctgaaagt gtttgtgggt
     3361 caactcaacc ctgtagtaaa ccaatcccgt caatcagtgc accgcgccac catgcgtaat
     3421 cgtgaccacc ggtgtagatt gcgcaatggc tttcgacgcc agcggtaatc agccgctggt
     3481 gaagctgctg aacgtgaggc actgtcgaac cttccagcga tcccacgcac aggctgatac
     3541 gtacgccctg cggtggggca gaaagcagat gctcactcac ccatgaggta tcggtttcgc
     3601 tgaacaagcc tggtcgactg gttctttctg gcgtccacca cattgaagga gagtggctga
     3661 gcaccagacc gaacgtttcc ggtgcgtaac gagcccccat tagcgcactg atcccgccga
     3721 ggctctgccc ggccagcact gtgcgcgaac ggtctgccca ctgacgctgc ggttgttcag
     3781 cgcgaatcat cggcagcaga tgtccggcga tatcctttat cagtttgctg cgcccgccga
     3841 gtatctcagt gcgttcatgt tcattaatgt tgtcaatgcc cagtacagcc acgggcacga
     3901 tgcgcccgtt atttatggcg gcgtcaattg ccgcgcatac gccaaggtga tcaaaccagg
     3961 tttcaccatc gggcagcacg accagcccga ggggctgaga aatatcaaca tccggaatat
     4021 agagccgaac ctggcgagat ttcccggcga taatgcgcat ggaggtaagc agttgtccgg
     4081 tgtggaagcc gccgttccat tccgacaggg cgggagcttt atcaagtgtc agcactgatt
     4141 cctttgcgtt cccccgaacg ttgatctccg gcattttgtt tagcggatcg gcttttccgg
     4201 cgagggtggc aaaacggcct ccggacagtg caatcgtctc agccgtagtg ccggggggga
     4261 tttccagcag cgaataggag ccgcagtaac ttgcgggtaa acgcagtgtc agtgtccaga
     4321 tatccgtttc gggaagggcg ctcatcattc ctttttctac gtgctcttta tccgtcaccc
     4381 ggttcagacg cagataaacg ccctgcagag gatgtcgggc gcgccacaga aaggtcactt
     4441 ctcgctcatc ttccgctcct tcgactggcg tcacaagagg aacattcagc gactcagctt
     4501 cgcgccagaa gtcagtaagg ttagcaatgt ggcccgctgc cagttggttg cgcaggctgg
     4561 cgatagcaga gggatgttgt tgcatgttca gcattcatac tctttaaaaa ttaaggatgt
     4621 cagtataaat aattaaagcg gctattcacg gtaccgccgc ttctgactgt gtttttaata
     4681 cattacgtgt ccagtaacta tcccggggtt tcctccttca tgttgtgctg gcgatggagc
     4741 gcagtactgc tgacgctgtc atgccataag cgggtataga ggccgcccgc agataacagt
     4801 tgttcgtggg ttccgtattc cgcaagtaac cccttatcaa taacggcaat cagatcgcag
     4861 cgttgcgcgg tggtcagacg atgtgcaaca atcagcgccg agtgcttccc ggcatgcacg
     4921 acatctgtta acgatgacat cagtcgttct tcggatgtgc gatccagaca tgacgtggct
     4981 tcgtcgagca gcaggatgtg agcattcgcc agttgcgcgc gggccagcgc aatcaactgg
     5041 cgctgacctg cagacagatc ggctccgccg ttattcactg gtgtccggaa tccctgcggc
     5101 agattgcaca ccatctcata cagccctgcc cgctgtgagg caatttcaac gtcttcattg
     5161 gtggcggatg gccgtgaata acgaatgttt tctgcaatat cactactaaa cagtgctaca
     5221 tcctgatcga caagcccaat ctggcgacga tactcagtga gcgaggcatc atcgagcatt
     5281 tgcacaccga ttctgatgtt gccgtgcgtg gggaaataca acccggcaat cagcttaatc
     5341 agcgtcgact tacccgcacc gctgcgaccg acgaccgcga caaccattcc ctctgggatc
     5401 gtcagggtaa gtttgttcag agctggctcg tgactgtcgg gataactgaa cgtgacctca
     5461 tcaagatgca gtgcaccggt gacagggagg accgaagagg agccgaggtt ctcagtgcct
     5521 tctgtcgcca gtagttcatc aatatgtttg ccgctggctg tcgcctgctg ccaggcgtcg
     5581 acaatccctg ataactgctg cactggccca tagaattgcc ccagcaacag gaagaaagcc
     5641 gccagtaccc cggcagtcat ttctccagcg gcgacctgcg aagctcccac taacagaaca
     5701 gcggcatagg aggcctcggt gcagaatgtc aggaacggaa aatagactgc aaggtatttt
     5761 tgtgctcgca cacgggttgc gcggaaacgc tccgataacg cgcgcagccg ggcgccctcc
     5821 agttcctgct gaccatgcga ttgcacgaca cgcaggccgg agactttttc ctgcagggtg
     5881 ctgttgactt tgcctatttc cagccgtgcc tgtgcataag caggggtact gaggcggcga
     5941 taaatcatgg ttgccagtgc ggccactggc actgcgctta atgccgtcag cgccaggaac
     6001 gggtcgagcc agaacatggt ggcggcgatt gcgaacatcg tcaccagact ggtggccgca
     6061 ccggcaaggc cgttttgcag aaagcgggcg agagagtcca catcaacggt catgcgggta
     6121 agacgcgaat cggcatgctt ttcatgccag gggagtccaa gacgcagcat atggccgaag
     6181 ctgcgcaagc gcaccgaatg ctgaactgat tccgccgctc tggcggcgaa gaccgtctgc
     6241 agagaataac tgcaccagcc cacaatgacc aggcacaggg cgagcagggc acagaggccg
     6301 atggtcgaca tatcgcctgc ggcgacaccg gagtcaatgc cgtgctgcaa cagtatcagc
     6361 acccctacgc ctgccgcaga atcgagtgcg atcagcagag cggcgatgac gaacatccac
     6421 gccacaggct tcagtagtga cgtgactttg cgacctttac cggccagcgc ctgctgtact
     6481 gcattttccg gtacgcgggt catacgggcg acaaaacctt tatcccccgt atccgttaca
     6541 tcatcttgtg tcgctggttc agccggccac aattcgttgt ggctattgtg ggacgtggcc
     6601 agaaaatcgc tgtcgccggt catcagtgcg cggaacgccg gacagcgcgc ttcaagttct
     6661 gccggggttc cggtatccac catacggccc ttatccagca ccacaacccg gctggctagc
     6721 tgaagtgttg aacgccgtcg ggcaatcacc agcagcatat gcccttcgtc agcatagcga
     6781 cccagcgcgg tattaatctc cgcttccgta acagcatcaa ccgctgaggt ggtatcatcc
     6841 aggattaaca cgtccggtgc agtaatgagc gcccgcgcca gcgctattcg ctgcctctgc
     6901 ccgccggaaa gattcgtacc ccgttcggtt aacaggctat cgaagccttt cggcagggcg
     6961 ttgataaaat cgctggctcc tgcagcagct gccgcacggc gaatgtcgtc cgccgttgcc
     7021 tgagggtggc cataggcgat attttccgcc accgtaccgg caaacagaaa agcatcttca
     7081 aatacgatgc ctacccggcg acgaagcgcc tccagtctga tatcgcgaag attttgtcgg
     7141 ccttcgctgg tgttgagcca tatctttccg gagcagggat cataaaaacg cgccagcagc
     7201 atcaacaggg tcgattttcc tgaacccgat gcgcccacca ccgccacggt ttccccagcg
     7261 cgcagggaaa aggagatatc gctgaggata tgtctgtcgc catggtggta gtcaaagctc
     7321 atattctcca gttccagtcc gacaacctga ctgttaatcg actccgtccc gtcctccatc
     7381 tgtgaacggg tgtcgatcag tgcaaacacc cgctccacgg acgcctgagt gcgctgtgcg
     7441 ataaccagaa acgatgccag tacgcgggtt ggcccggtca gcatcgcgag gaagctggcg
     7501 aatgcaacga aggtaccgag atcgatccgc ccgtgcatga ccgaccaccc gccgtagcac
     7561 agcaggacga tttgcccgag caccggtagc gccagcatag tggccccagg catcgcctgc
     7621 gagataaccg caccgatgcg tacacgcaca atctgccgcg actgcctatc gagccagtgc
     7681 gtttcacgca tctctgccac acaggatttt accactgaga tctgtgccag cacctcgcgt
     7741 atatgttcgg tgagattcgc cagttggtcc gaggccattc cggtttgcgc gaagacccta
     7801 cgacgcgcac gaagcgcggt gatcgccagg cataccagta cgcacacgac gataagcgtc
     7861 atggcagggg acatccacag catcacggca atgccggcaa tgtaataagt gaacactgcc
     7921 agcggcaccg ggcacatctg cagcagggta tgcacctgct gcagatcgct gttggtccgt
     7981 gaaatcacct gcccggtgcg cagcgcatcc tggcctgcgc cgtcaagttt ctgaatactg
     8041 tcgaataccc gtcctctgag ggtatgctgc acccagagtg agagcatccc ggcataacca
     8101 cggcgcacat agttaccgat gaaatcaaaa aaagcgatca acagtaaccc gcaggccagc
     8161 cacggcaggc gggccggatt gcccgccagt gcgtcattca cagcctgtct tgtcagcagt
     8221 ggcgtcagcg cagccagcaa aatagtcgat actgacgcta ccaccacgat gacactaagt
     8281 ttcttacgtt cccagcacac gcgcgccagg cgaactatcc atgactgagc cggtgtggga
     8341 gtgtgattcg caggcattaa gcctcctccg tctgtaaaat acgatccact ggccggatcg
     8401 ttccgcaaaa aagccagcac actccggtcc cgcattgtta tggatgcatg actcctggat
     8461 gggggctggc aaatgattat catgaaggtg tagtggctac cctttctgta ccattgtgat
     8521 caggcttttc gccacttcac cagggcaggg ttgcgccgcc atttccgctg ctacctcttc
     8581 agaggctttg cgaagtgagc gattgttgag gaaagcattg atcatgttac tcgacaggcc
     8641 gacgtcgccg gggataatcc cgcagccgcg ttcggcaaca acgcgggcat ttaccgggcg
     8701 atcggctccc tggccgaaga ctatttgtgg aataccagcg tgtagcgctg tcagggtatt
     8761 gcctgctccg ccatgatgaa tgaaaccatc tgcgccgttg aggaagacac ccatgggtat
     8821 ccagtcaacc aggcgaacat ttgatggcag cgagcgcaaa tcggaccgcg cattggctga
     8881 aatatgcagg atgatctcgg catcgacctc actggcagaa tccataaccc aggcgattag
     8941 atcaaggccg tcaaccatcg gtttgacagt gccgagactc accagaagac gtttacgatc
     9001 gggccttctc tcccaccatg gttcccacac cgcaccaccg ttatatggga cgtactgcat
     9061 cggaataatg ggttcaccgt cgttttccag aatgctcata ctcggcggtg ttacgtcgat
     9121 ccaggccata tcgcgtggtg tggccccgac attgtggcga cgataggcgt ccgtgagcga
     9181 acgcgttaca cccctgatat gccagggggt atggccgaag cctacggttt gcatcacgac
     9241 cggtatatca tatttggcgg caatcagcgg cccgatgact ccgagtggag ggtagatgat
     9301 gaggtcaggc cgccagtgtc cggcaaactc caccagatgg tctgccattt cttcgctgaa
     9361 gaatgagaag ttgcccattt gggttccgat gttacttttt ttccgctgtg cctcatggtg
     9421 gcggtaacca gcttcggaat ccagaccggg ggcagcgtca aacaccacca acccggcctc
     9481 tgccgctttt tgtgcgaatt gtcctccgct ggcgatcagt acttcatgac cgttaacgcg
     9541 aaatgcctgc gccagggaca gcacaggata caacagccca tagagaggcg ggcctacaaa
     9601 caaaatacgc atgagaaaat ccctctccgc ttgacgatct aaatcaggca gtcgacgcac
     9661 tcatatgcca gcagcaggac acgacttttc gttaaacctg ctttaccaga tgctgcgcca
     9721 tttgtgcttc accagcgtta tggacagatc agtcagagac ttttccgtga catacacagg
     9781 tgaacaggga actccgtatc cccacttcca ttaacgctta cgcgccattg tcctgttgaa
     9841 catcccggcg tcggacagct acgccgggat gttctgacgc atttgtgcag tgttagaccg
     9901 acaatgaatc tggaaagatc cgtgactttt tagacacacg gcttgcatca tcctgatagt
     9961 ctcgccttcg ctggcctgta agtaaaatga ccttgtactg gcgatgcgtg ttccctttca
    10021 gccttaccag agacaacgtt agcgcaaatg ctaatgataa taattaccaa tatcattaag
    10081 gtattgcaac gataattaaa aacttaatga aaagggaagc actcagagga ctatccgggg
    10141 gtatttaaat tgaaacctac gctgctccag accgtggaga atatttgcct gcaaattctg
    10201 ttcgtgctgt tcctgttcac cggaacgctg atagacgttg aggcattggt gagctgttct
    10261 tccgttgcca aaaatgtaca gagaactccg gccagcagcg tttcatcctc tgtctatagc
    10321 gcaagcgtgt cgagaactgc tgctgatatt atgctgttgt cgaataatcc gctttttgct
    10381 ccgggtcagg tgagtttttt cggctgttcg ttggcagcgt caggtattca cgtcaccatt
    10441 ctggagtaat gccagtcaat taagcaactg acaggct
//
LOCUS       DADTFG010000159        10325 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.160, whole genome shotgun sequence.
ACCESSION   DADTFG010000159 DADTFG010000000
VERSION     DADTFG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.160"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            100..642
                     /gene="ecpR"
                     /locus_tag="J1138_003924"
                     /gene_synonym="matA"
     CDS             100..642
                     /gene="ecpR"
                     /locus_tag="J1138_003924"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HBA8455661.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            716..1303
                     /gene="ecpA"
                     /locus_tag="J1138_003925"
                     /gene_synonym="matB"
     CDS             716..1303
                     /gene="ecpA"
                     /locus_tag="J1138_003925"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HBA8455662.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1360..2028
                     /locus_tag="J1138_003926"
     CDS             1360..2028
                     /locus_tag="J1138_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HBA8455663.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTHVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2054..4579
                     /locus_tag="J1138_003927"
     CDS             2054..4579
                     /locus_tag="J1138_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455664.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSMSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4569..6212
                     /gene="ecpD"
                     /locus_tag="J1138_003928"
     CDS             4569..6212
                     /gene="ecpD"
                     /locus_tag="J1138_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HBA8455665.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6181..6891
                     /locus_tag="J1138_003929"
     CDS             6181..6891
                     /locus_tag="J1138_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HBA8455666.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPSGGEVSTEPVVVMDSILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7198..7527
                     /locus_tag="J1138_003930"
     CDS             7198..7527
                     /locus_tag="J1138_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBA8455667.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGQNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7539..7702)
                     /locus_tag="J1138_003931"
                     /pseudo
     CDS             complement(7539..7702)
                     /locus_tag="J1138_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7776..8390)
                     /locus_tag="J1138_003932"
     CDS             complement(7776..8390)
                     /locus_tag="J1138_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HBA8455668.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8839..8997
                     /locus_tag="J1138_003933"
                     /pseudo
     CDS             8839..8997
                     /locus_tag="J1138_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9214..9907)
                     /locus_tag="J1138_003934"
                     /pseudo
     CDS             complement(9214..9907)
                     /locus_tag="J1138_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2831 a         2568 c         2505 g         2421 t
ORIGIN      
        1 atttactaaa gaaactgaat gtacctgtaa aaattacagg tttggaaagt agtgacatgg
       61 caaaatgatt acagcaggga ctatgaggtt aaaaaccata tggaatgtca aaaccgttct
      121 gataaataca tctggtctcc ccatgacgcc tacttctata aaggactatc tgaactgatt
      181 gtggatatcg acagattaat ttatctatcg ctggagaaaa tcagaaaaga tttcgtgttt
      241 atcaatctca atacggattc tttaactgag tttataaacc gtgataatga gtggttatcc
      301 gcggtaaagg ggaaacaggt cgtattgatt gcggccagaa agtcagaagc cttagcaaat
      361 tattggtatt acaacagcaa tattaggggc gtggtatacg ctggactgag tcgtgatatt
      421 agaaaagaac tggcctatgt gattaatggc aggttcctga gaaaagatat taagaaagat
      481 aaaatcactg accgggaaat ggaaattatc cgcatgacgg ctcagggaat gctgcctaaa
      541 tcgattgcca gaattgaaaa ttgtagtgtg aagacagtgt atacccatcg gcggaatgca
      601 gaggccaagc tgtactcaaa attatataag ttggttcagt aaactccagg caagttagtt
      661 ttaaaaatga ctcaccggga cttcatgtcc tcaattcaac tcgggaagaa aagcaatgaa
      721 aaaaaaggtt ctggcaatag ctctggtaac ggtgtttacc ggtacaggtg tagcgcaggc
      781 tgctgacgta acagctcagg ctgtagcgac ctggtcagca acagccaaaa aagacaccac
      841 cagtaagctg gttgtgacgc cactcggtag cctggcgttc cagtatgccg aaggcattaa
      901 aggttttaac tcacaaaaag gtctatttga cgtggctatc gagggtgact caacggctac
      961 cgcctttaaa ctgacctcac gccttatcac caacacctta acccagttgg atacctcagg
     1021 ttccacactg aatgtgggcg tggattataa cggcgcggca gtcgaaaaaa ctggcgatac
     1081 cgtgatgatc gataccgcca acggcgtact gggcggcaac cttagcccac tggctaacgg
     1141 ttacaatgcc agcaatcgta ccaccgcaca ggatggtttc actttctcca tcatcagcgg
     1201 caccaccaat ggtaccaccg cagtaaccga ttacagcact ctaccggaag gcatctggag
     1261 cggcgacgtt agcgtacagt tcgacgctac ctggaccagt taatctctct gatgtaccag
     1321 caggggtacc cccctgcttt attcctggac ggaattgtta tgaaaaagca ccttctgcct
     1381 ctcgctctgc tgttttccgg aatatcaccg gcccaggcgc tggatgtcgg cgatatatca
     1441 tcgtttatga acagtgacag cagcacgcta agcaaaacga tccaaaacag taccgacagt
     1501 ggccgcctca tcaatatccg tctcgaacgg ctctcttcac cgcttgacga cgggcaggtt
     1561 atcgcaatgg acaagccgga tgagttgcta ctcactcccg ccagcttgct gctacccgcc
     1621 caagccagcg aagtgatccg cttcttctat aagggaccag cagatgaaaa agagcgctac
     1681 taccgcattg tctggtttga tcaggccctc agtgatgcgc agcgcgataa tgccaaccgt
     1741 agcgctgtgg ccactgcttc cgcccgcatc ggcaccattc tggtcgtcgc accccgccag
     1801 gcgaactacc actttcagta cgccaacggc tccctgacaa atacaggaaa tgcgacgttg
     1861 cggatcctcg cctacggccc ctgcctgaaa gccgccaatg gtaaggagtg taaagagaat
     1921 tactacctga tgccgggcaa gtcgcgtcgt tttacccacg tggacacggc ggataacaaa
     1981 ggacgggttg cactttggca gggtgataag ttcattcccg tgaaatagat agctgtgcag
     2041 atggataacg acaatgcctt tacgacggtt ctccccagga ctgaaagccc agtttgcctt
     2101 cggcatggtc tttttgttcg ttcagcccga tgccagcgct gctgacataa gtgcgcagca
     2161 aataggtggg gtgattattc cgcaggcctt cagtcaggcg cttcaggacg gcatgagcgt
     2221 cccgctctat attcatctcg ccggtagcca gggtcgccag gacgatcagc gaatcggcag
     2281 cgcttttatc tggttggatg atggacagtt acgcatccgg aaaatacagc tggaagagag
     2341 tgaagataac gccagtgtca gcgaacaaac tcgacagcaa ctgatgactc tggccaacgc
     2401 cccgttcaat gaggccctta ccatccccct gactgacaac gcgcagttgg atctcagctt
     2461 gcgccaactg ctgctgcagc tggtggtcaa gcgcgaagcg ctgggcaccg tactacgctc
     2521 acgtagcgaa gacatcgggc agtccagtgt taacaccctc agcagtaatc tgagctataa
     2581 cttcggcgtc tataacaacc agttgcgtaa cggcgggagc aacacctcca gctatctgtc
     2641 gctgaataac gttactgcgc tgcgcgaaca tcacgtggtg ctcgacggct cactgtacgg
     2701 gattggtagc ggtcaacagg acagtgaatt atataaagcg atgtatgaac gcgattttgc
     2761 tggtcaccga tttgccggtg gaatgctcga cacctggaac ttgcagtcct tagggccgat
     2821 gaccgccatt tcagcaggga agatttacgg cctttcctgg ggaaaccagg ccagctccac
     2881 catcttcgac agcagccaat cggccacgcc agtgatcgcc tttttaccgg cggcgggcga
     2941 agtacatctc acccgtgatg ggcgactact aagcgtgcag aacttcacca tgggcaatca
     3001 tgaagtggat acccggggtc taccgtacgg tatttacgat gtggaagttg aggtaatcgt
     3061 taacggtcgc gtgatcagca aacgcaccca gcgggtcaat aagctgttta gccgggggcg
     3121 tggcgtcggt gcaccgctgg cgtggcagat atggggcggt agctttcata tggatcgctg
     3181 gtcggaaaac gggaaaaaga cgcgaccagc caaagagagt tggctggcag gtgcctcgac
     3241 ctccggctca atgagtacgt ttagctgggc ggcaacggga tatggatacg ataatcaggc
     3301 ggtgggtgaa acccgtctga cgctgccgct tgggggggcg atcaacgtta acctgcaaaa
     3361 catgctggcc agtgacagct catggagcaa catcgccagc atcagcgcca ctctacctgg
     3421 aggcttcagt tcgctgtggg ttaaccagga aaaaacccgc attggcaatc aattgcgacg
     3481 tagcgatgcc gacaaccgtg caatcggcgg cacactcaac ctgaactcac tgtggtcgaa
     3541 gctgggtacg ttcagcatca gctacaatga tgaccgccgc tacaacagcc attattacac
     3601 ggcagattac tatcaaagtg tctacagcgg tacctttggt tcgcttggcc tgcgggccgg
     3661 tattcagcgc tataacaacg gcgacagcag cgccaataca gggaaatata tcgctctcga
     3721 tctctcgcta ccactgggca actggtttag cgcagggatg acccatcaaa acggctacac
     3781 catggcaaac ctgtcagcac gcaaacagtt tgatgaagga accattcgca ctgttggtgc
     3841 caatctgtca cgagccatct ccggcgatac cggtgatgac aaaaccctca gcggtggggc
     3901 gtatgcacag ttcgacgctc gttacgccag cggaacgctg aacgtcaata gcgcggcgga
     3961 cggctacatc aatactaatt tgaccgccaa cggcagcgtc ggctggcagg gtaaaaacat
     4021 cgctgccagc gggcggaccg atggcaacgc tggggtgata ttcgacactg gcctggagaa
     4081 cgatggtcag atcagcgcca aaatcaacgg gcggattttc ccgcttaacg gcaagcgtaa
     4141 ctatctcccg ctctctccct atggaagata tgaggtggag ttgcagaaca gcaaaaactc
     4201 actcgacagt tacgatatcg tcagcggtcg caaaagtcat ctgactctct atccaggcaa
     4261 tgtcgctgtc attgagccag aggtgaagca gatggttacc gtctccggtc gtatccgtgc
     4321 ggaagacggc acactgctgg ctaacgcacg gattaacaac catatcggcc gaacccgaac
     4381 cgatgaaaac ggcgagtttg tcatggacgt ggataagaaa taccccacta tcgattttcg
     4441 ctacagtggc aataaaacct gcgaagtggc actggaactc aaccaggcgc gcggtgccgt
     4501 ctgggtcggt gatgtggtct gcagcggcct ctcatcgtgg gcggcggtga cgcagacagg
     4561 agaagagaat gagagttaac ctactgatag cgatgataat ctttgcgcta atctggcctg
     4621 caaccgcgct cagagcggca gtgagcaaaa caacctgggc ggatgctccg gcacgcgagt
     4681 ttgtgtttgt cgaaaacaac tcagacgaca actttttcgt cactcctggc ggggcgctgg
     4741 atccgcgcct gaccggtgcc aaccgctgga ccggtttaaa atacaatggt tcaggaacca
     4801 tctatcagca aagcctcggc tacattgata acggttacaa caccggcctt tataccaact
     4861 ggaagtttga tatgtggctg gaaaattcac cagtttcatc tcctttaact ggcttgcgct
     4921 gcatcaactg gtacgcaggg tgtaatatga ccaccagtct tatcctgccg caaaccaccg
     4981 acgccagtgg attttatggc gcgacggtga ccagcggcgg cgcgaaatgg atgcacggca
     5041 tgttgtcaga cgcgttttac cagtatctgc aacaaatgcc cgtcggcagc agctttacaa
     5101 tgaccatcaa tgcctgccag acctctgtga actatgacgc cagcagcggc gcacgctgta
     5161 aggatcaggc ctccggcaac tggtatgttc gcaacgtcac ccatacgaaa gcagcaaatc
     5221 tgcggttgat aaatacccac tcgctggcgg aagtatttat caacagcgac ggagtaccga
     5281 ctctgggcga agggaacgcc gactgccgga cgcaaaccat cggcagccgt tcaggattaa
     5341 gttgtaagat ggttaactat accctgcaaa caaacggact cagcaacacc tcaatccata
     5401 tattcccggc gatcgccaac tcgtcgttag cctcggccgt cggggcgtac gatatgcagt
     5461 tcagtctgaa tggcagttca tggaaaccgg tgagcaatac tgcctattac tacaccttca
     5521 acgagatgaa gagcgcagac tcgatctatg ttttcttctc gagcaacttc tttaaacaga
     5581 tggtgaacct cgggatcagc gatatcaaca ccaaagatct attcaacttt cgctttcaga
     5641 acaccacatc accggagtct ggctggtatg aattttctac ctccaacacg ctgattatca
     5701 aaccccgtga tttcagcatc agtattatct ccgatgaata tactcagaca ccgtcgcggg
     5761 agggatatgt tggcagcggc gagtcggcac tcgatttcgg ctatatcgta accaccagcg
     5821 gtaaaacagc tgccgacgaa gtgctgatca aggtgaccgg acccgcgcag gtgattggcg
     5881 ggcgctccta ttgtgtcttc agctccgatg acggtaaggc gaaagtaccg ttcccggcga
     5941 cactttcctt tattacccgc aacggagcta caaaaaccta cgatgccggg tgcgatgata
     6001 gctggcggga tatgaccgat gcgctgtggt tgaccacacc gtggactgat atctctggcg
     6061 aagtggggca gatggataag accacagtca aattttcgat tccaatggat aacgccattt
     6121 ctctgcgtac ggtagatgat aacggctggt ttggcgaagt cagcgcttca ggagagattc
     6181 atgttcaggc gacgtggcgt aacattaact aaggccctgc tgacagtggt ctgtatgctg
     6241 gcggcacctt tgacacaggc gatttcggtc ggcaatctga ctttttcact gccgtccgag
     6301 actgactttg tcagcaaacg tgtcgtgaat aacaacaaaa gcgcacggat ataccgtatt
     6361 gccatcagtg ctattgatag cccgggcagc agtgaattgc gcacccgacc ggtggatggt
     6421 gaactacttt tcgccccccg ccagctggcg ttgcaggctg gtgagagcga gtattttaaa
     6481 ttttactatc atggtccacg ggataaccgc gagcgctact accgggtctc atttcgcgag
     6541 gtccccactc gtaacctgac aaaacgcagc ccaagcggcg gtgaggtcag cacggagccg
     6601 gtggtggtga tggatagcat tctggtagta cgaccacgtc aggttcagtt taaatggtcc
     6661 ttcgatcagg tgaccggaac ggtaagtaac accggcaaca cgtggtttaa gctactgatt
     6721 aaaccaggat gtgattcgac cgaagaggaa ggcgatgcct ggtatctacg tccgggagac
     6781 gtggttcatc agcctgagtt acgtcagccg gggaatcact atctggtcta taacgacaaa
     6841 ttcattaaga ttagcgattc ctgtccggct aagccccctt cggcggacta agacttatcc
     6901 atcggacgag ggaagaattc cacctcagag actcaaaaat cgtttaaatg atgtggaagc
     6961 gatcgtcaat gtcaccgatg agctttctca tatgcacacc acagtgttca ggagacacgg
     7021 aaaggtacca ggcggcctgc taacgagtgg tttcttcact aattaggacg taaaatcggt
     7081 aacggctgga aatcattcaa tactcacact atcgaaagta caccagccaa ccgcgatacg
     7141 tccctgcata cgacgtaccg cagttctctt tacgatttat agctgtactg gtgaattatg
     7201 agcaatctga atccatgcat gacgtgtggt gcctgttgtg catttttccg cgtctctttt
     7261 tactgggccg aagccgacga tgctggcggt accattcccg ccaggctcac tgaacaaata
     7321 tccccttttc accgatgcat gagcggtact aatcagaaaa atccccgatg tattgccctt
     7381 gcaggaacac cgggccaaaa tgcctgctgc acgatatatg aaaatcgatc gtccacatgc
     7441 agagaattcg ccatgtctgg tgagaacgga gaagtcaatg aggcttgcaa tcgtgcaaga
     7501 gctaaatacg ggctaacacc gttataaata tacaataatt aattgcattg ccccgccagc
     7561 tgtaatagcg gggcttcgtt tttcagggac aacaaaacct gaaacttctt ccattttcca
     7621 gcaacaatgc ctcttcttct gtagcgttct ccggatctac atcatcctga agtagcgcga
     7681 tatgatcact gactgtccgc atcagaatat gaaaagcaac gtttttaacc tataaaaatg
     7741 gcgctatatt tgcgccattt ttatcattca aagcattatc tatttgagcc taaagggatc
     7801 tcaggatctg gctcatgggt aattctgttt cgtaaatctc tgcgaataat ttcaatagac
     7861 cagaaccaga ctaaatgtcc aaaaatttca gagacattct cataccacgg aagatcaaag
     7921 agaggtggtg taagtcccat gagagggaat gaaatcatat gaacaaaaag ttgcgctaaa
     7981 gcacctgcca gtaagccctg ccaaagttta attttcggaa atacttcggc gaccacacag
     8041 taaccaacgg caaaaactat cgaaaagatt atgtgcgtta caccaaccca gttaaagaca
     8101 tgcccggcga aggtataaac agccgaattt ggatctgtca gtcccaacca atcccgaaga
     8161 aaaatgtacg gaggattgag aaagttacgg gagcaatcaa tttggcctgc agccctgatt
     8221 aatgattccg gaccacacgc tgcattaaac atatcaaccg gactacgtgg tggcaatgga
     8281 acttctgccc cccacttaac aaatgcggaa acaacgccag caatcagccc aatgaatgca
     8341 gcaagaccat aacgtctgcg gtttggtgga gtttgttcaa atatattcat atctaccctg
     8401 cttgtaccat tatgttatac acctctttag gagtattcat aaaacaaggc aaatgtaaag
     8461 aactgtattg ttttgtataa ccacatggtt acctaatcgc caattaatat aagctccatt
     8521 atttctccat atttttatgt tagaagtgac aaggagaaat gtaatgtttc tctcagagaa
     8581 tataaatcat tccttttgcc caccttaatg gtgggctttt ttatgcaaaa cctgaacgtc
     8641 ctgtggtatt cctcaggcat aatgattttc gcgattaacc aacaaccaga ccagcaaaga
     8701 cgccaccaca agcacagtaa catccatcag gcttgccaca ccccatgcgt gcggctcaaa
     8761 gccgccatgg catattaatc cgcttgccat tttcgaacag ctgattggtt tggtccggtg
     8821 tttccctgct tgcacttaaa aatgagcgga taggaaactc tcgaaatggt tcgtcgatat
     8881 gctcatctcg cacctaatca ccttaccgaa cacgcacggt aaatagattc gatcctgaac
     8941 ccatcggtcc caaatctgtc ccaatcaaaa aataaggaag gtattaacga tgtgtaacct
     9001 attgatttaa aatggataat ataaacagat tgtaaaaagc aagacattga tatgtatata
     9061 aaaaaactat tacgcaatca ttagataccc ccagatttag ttgtaaagca agcacacttt
     9121 gcatttcgag acaatccagg taaagactct tacctcccat tttgtgattt ttatacttat
     9181 gatgataagt tgaacgttgg gtagtgcttc caatcagtag aacatgtgct tttcaatgaa
     9241 ggaaccgatg actttttcgt ggatttcaac ggaacgcgag aagcagattg ttttacttac
     9301 cagccgcttg atgcgggtgc ggagggtcaa gttgttacgt tcaatacgtt gcgtgaagat
     9361 tttgccggtc aggtatttct ccttcggcac ttctctggcg tagctgcccc agtcatcgcg
     9421 gccaccatgc cgatacagaa cggagtcaac agggctaaca attcccgaca ggcctcatca
     9481 ttccgtggcc cgaaagtgta agccaacaca ccgccagttt tagtgttcta cgcataccag
     9541 agccagtgtt gttgcgcctt gttgccaacg aagctccaca gctcatcaag ttcacagata
     9601 agggcaacat cagcgtgagc tgactgacgc agatgttatc cgctggggcg agagcttttt
     9661 aatgtccgta tgacggtatt gatgctgatt ttcagtgttc tggcgatatc acggcccctg
     9721 tgccattatg cgccatttca acgatcagct ccttaaagcc gggttttctg gcttcataag
     9781 agtaagtgag ctggaaaaca cgtttacatg aacgacaacg gaaacgctca tgctgggaac
     9841 aactacgacc atgtcgataa actttatcgg aattacagcg cggacgatga acagtaacgg
     9901 tagccatgga aaaccttaaa taccagtaga ttaccagaaa cttctatcaa ttgaagacat
     9961 taccgttttt gattacagca aatgttttat taattaccca accatcgact aaatccgtta
    10021 gaacgtcgaa tgatgccatt cgtaaataca ttaaaaagac tgttgtaatc aacgaagatg
    10081 gaaaacagtg tgttaaaact aatctgaact tcagaaaaac agatgatggt atggtgatat
    10141 atatatccgc tctgaaccgc cacgcaattt aaaataaaaa tcaacaaata tcgtcgatat
    10201 ccatatatac catttttctg tagttgtatt agcgtcgcat attttatgac tttaagtcat
    10261 aagtcaaaac acctgagaaa aagcaaacaa cattttcatt accaccaatg aaaaccacag
    10321 acaaa
//
LOCUS       DADTFG010000160        10287 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.161, whole genome shotgun sequence.
ACCESSION   DADTFG010000160 DADTFG010000000
VERSION     DADTFG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.161"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(11..1429)
                     /gene="phrB"
                     /locus_tag="J1138_003935"
     CDS             complement(11..1429)
                     /gene="phrB"
                     /locus_tag="J1138_003935"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBA8455669.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVETVKQVCAENRITHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(1426..1935)
                     /locus_tag="J1138_003936"
     CDS             complement(1426..1935)
                     /locus_tag="J1138_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBA8455670.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDQTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            complement(2036..2242)
                     /locus_tag="J1138_003937"
     CDS             complement(2036..2242)
                     /locus_tag="J1138_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBA8455671.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            2555..2644
                     /gene="kdpF"
                     /locus_tag="J1138_003938"
     CDS             2555..2644
                     /gene="kdpF"
                     /locus_tag="J1138_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBA8455672.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            2644..4317
                     /gene="kdpA"
                     /locus_tag="J1138_003939"
     CDS             2644..4317
                     /gene="kdpA"
                     /locus_tag="J1138_003939"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBA8455673.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     VLFRLPGVSDSEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQLLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLAPVALLIALFFIQQGALQNFLPYQAVTTIEGSQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            4340..6388
                     /gene="kdpB"
                     /locus_tag="J1138_003940"
     CDS             4340..6388
                     /gene="kdpB"
                     /locus_tag="J1138_003940"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBA8455674.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            6397..6969
                     /locus_tag="J1138_003941"
     CDS             6397..6969
                     /locus_tag="J1138_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HBA8455675.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPNASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            6962..9646
                     /gene="kdbD"
                     /locus_tag="J1138_003942"
     CDS             6962..9646
                     /gene="kdbD"
                     /locus_tag="J1138_003942"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HBA8455676.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDAPPARTLNNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            9643..>10287
                     /gene="kdpE"
                     /locus_tag="J1138_003943"
     CDS             9643..>10287
                     /gene="kdpE"
                     /locus_tag="J1138_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBA8455677.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFIT"
BASE COUNT         2216 a         2742 c         2898 g         2431 t
ORIGIN      
        1 ctgcgtttta tcaggctcct ttcctcgctt cttcgtacgc tgccagcgtt cgcagccttg
       61 cctctttgtg atcgactatc ggttgcggat aatccaacgt cacacctgtt ttctctgccc
      121 acttccacgg ctcatgcacc gctttccccg gtacatcgcg cagctccggt agccactggc
      181 ggataaattc gccttcgcgg tcaaatttct cgccctgagt tgtcgggttg aaaatacgaa
      241 aatacggcgc tgcatcagta cccgtagaag cggcccactg ccagccaccg ttattagctg
      301 ccaaatcacc atcaatcagc tgcgacatga aatatcgctc gccttcgcgc cagtcgatca
      361 acaaatcttt caccagaaaa ctggctgtaa tcatccgtag ccggttatgc atccagccag
      421 tgctgttaag ctgacgcatg gcagcatcga caatcgggta tcccgttttg ccttcctgcc
      481 aggcctttaa atgggcggga ttactttgcc actgtacacg atccgtccag gcaataaacg
      541 gacgatgttt acacagcgaa ggataatacg tcatcaaatg gcggtagaac tcgcgccata
      601 tcagctcatt aagccagacg ctccccggtc cgccttccag cacctgcggt tgttccgcca
      661 acaggcgatg taaacactgg cgaggcgata acccgcccgt tgccaggctg gcggacaaac
      721 gactggtgcc ttccactgcc ggaaaatctc gttgttgctc atactctccg gcaccgttct
      781 ggcaaaactg gcgtaattgc gcaatcgccg ctttttcttc caccgggaaa tgcgcagtat
      841 cgaaagactg acgaggataa ttcagcgtaa tggatggcgc gggctctatc gatccgctac
      901 tacgaacttt tggtgcagca acgcactccg gcatcccttc ccgcagccgt ttcaaccatg
      961 cgtttttaaa aggcgtaaag actttgtaca tctcatgatt gccagtcatc accgcccccg
     1021 gcggcaggat cacgctgtca tcaaatcctt cacacaccac gttacgcagc gtcctctcag
     1081 cctgcacatc ccgcgcccgc tcattcacct catactgata gttataaaac agatgggtaa
     1141 tgcgattttc cgcgcacacc tgtttaactg tttcgacact ggcagcaaag tcatccactt
     1201 cgcggaacaa taaaggaatg cctttttccg ccagcgcgtt ttgcagcgca ttaagttgtg
     1261 tattgataag ttcagcctga cgcggcgaca cattatgcgc cgcccactgg cgtggtgtag
     1321 cgatatacaa cgccagcacg cgcgcagacg aattgcggca ggcggcagcc agcgcgagat
     1381 tatcgtgctg acgtaaatcc tggcgaaacc agaccagatg ggtagtcata aaactcctgc
     1441 atggcgcaag ttaatcacac gcggccaaag ttcgaaataa cgctgccctg aaagccaggc
     1501 gtcaggataa agcgccatat agtgtttgat acgcatcagc ggcgcaagaa gcggttgctc
     1561 gccacggcga taactgtcga taagcgccgc cagctgctgg cgttctgtgg aatcaatatg
     1621 tgggcgaaaa tatccctgaa cgtgcatcaa aacgttagtc tgatcacgcc ggttggcagg
     1681 gtgagaaagc aaaacaatca cgcgttggcg gtactggttg taatagtcat ccagactttg
     1741 ccaccggtga atatcagcca caaacgggcc aagtttgcgg tactccggct gagaatgcgc
     1801 cagaaagacg agtttatagc ggctgtgata atcgagaagc gccccgcgcg ttagtcctcg
     1861 atctttgaga ttgtgtaact catgcaaagc aaaaatgcgg ataagggtgc tgtcatcaaa
     1921 aagttgttga ttcattactg ctcctgaacg gtttacagga gtaagtgtag acgccgaaaa
     1981 aagaaaaccg ccagcgtttc caccggcggt tttctttaaa cgtcatgttt ggcaatcagt
     2041 aaaaatcacc ggttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg
     2101 accagacgcg gtgcaggtag ctataaaagc gggcgcggtc tttccagaaa agcatgaaag
     2161 gcagcgccag aacgcccgct gcaaccgcac aagtacggcg taaaaagata agccatgcag
     2221 gatattcttt gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca
     2281 ccggaaaaat gaattcgttt atctgatgaa aatagtaccg ctgtttgtgt aattttacta
     2341 ctcatccgac cacttatttt tgcttattga tggtttattt acattcaccg tgtaattaag
     2401 ttacataaaa gttaaattaa tactaagcat tagttaaatc atggcttttg ccatttttat
     2461 acttttttta caccccgccc gcagattttt gcgaaatctt tgcagccaga actctaccct
     2521 tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga tagccggcgt
     2581 gttgctggtg tttttattac tgggttatct ggtttatgcc ctgatcaatg cggaggcgtt
     2641 ctgatggctg cgcaagggtt cttacttatc gccacgtttt tactggtatt aatggtgctg
     2701 gcacgtcctt taggcagcgg gctggcgcgg ctgattaatg acatacctct tcccggtaca
     2761 gcgggtgttg aacgcgtact ttttcgttta cctggcgtct ctgacagtga gatgaactgg
     2821 aagcagtatc tctgtgccat tctcggcctg aacatgctgg ggctggcggt gctgtttttt
     2881 atgttgctcg gtcagcacta tctgccgctt aatccacagc tgttgccggg gctgtcgtgg
     2941 gatctggcgc tcaataccgc cgtcagcttt gtcacgaata ccaactggca atcttatagc
     3001 ggtgaaacca cgttgagcta tttcagccag atggcgggct taacggtgca aaactttctt
     3061 tctgccgcca gcgggattgc ggtgattttt gcgcttattc gcgcgtttac ccgccagagc
     3121 atgagcacgc tcggtaatgc ctgggtcgat ctgctacgca tcacgttatg ggtgttagcc
     3181 cctgtggcat tgttgatcgc actgtttttt atacaacaag gtgcgctgca aaactttttg
     3241 ccttatcagg ctgtgacgac cattgaagga tcgcaacagc tgttaccaat ggggccagta
     3301 gcttctcagg aagcgatcaa aatgctcgga actaacggcg gtggcttttt taatgccaac
     3361 tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcaaat gctggcgatt
     3421 ttcttgatcc caacagcgct gtgctttgcc tttggtgaag tggcgggcga tcgtcgccag
     3481 gggcgcatgt tgctgtgggc gatgtcggtg atttttgtca tctgcgtagg cgtagtgatg
     3541 tgggcagaag ttcagggtaa tccgcatctg ctggcgctcg gcgcggacag cagcatcaat
     3601 atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact
     3661 acggcggctt cctgcggcgc ggtgattgcg atgcatgatt cgtttaccgc tcttggtggc
     3721 atggtgccga tgtggttgat gcaaattggt gaagtggtat tcggcggcgt cggttccggc
     3781 ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct gatgattggt
     3841 cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg agatgaaact gactgcactg
     3901 gcgattctgg tcaccccgac gctggtgctg atgggcgcgg cgctggcaat gatgaccgac
     3961 gccggacgta gcgccatgct caaccctggc ccgcatggtt ttagcgaagt gctgtacgcc
     4021 gtgtcatccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc caactctcca
     4081 ttctggaact gtttactggc gttgtgcatg tttgtcgggc gcttcggggt gattatcccg
     4141 gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag ctccggcaca
     4201 ttgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc
     4261 gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggaatatct ctcctgatga
     4321 tattgagtga gcactgaata tgagtcgtaa acaactggcg ctattcgaac caacacttgt
     4381 cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc
     4441 ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca tcgcgatggc
     4501 aagcggtgtg atgcctggca atgcgctgtt tagcgcggca attagcggct ggctgtgggt
     4561 caccgtactg ttcgctaatt tcgccgaggc gttggcagaa ggccgcagta aagcgcaggc
     4621 caacagtctg aaaggggtga aaaaaaccgc ttttgctcgc aaactgcgcg aaccgaaata
     4681 tggcgctgcg gcggacaaag ttccagctga tcaactgcgt aaaggtgaca tcgtgctggt
     4741 agaagctggc gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga
     4801 tgaaagcgcc atcaccgggg aatcggctcc ggtgatccgt gaatccggcg gtgattttgc
     4861 ctccgtcact ggcggcacgc gtattctttc tgactggctg gtgattgagt gcagcgttaa
     4921 ccccggcgag acatttctgg atcggatgat cgcgatggtg gaaggtgcgc agcgacgtaa
     4981 aacgccgaac gagattgccc tgaccattct cctgattgcc ctgactatcg tctttttact
     5041 ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgcagtca gcgtaacggt
     5101 actggtggca ctgctggtct gtctgatccc aaccactatt ggcggcctgt tgtcagcgat
     5161 tggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtt attgccacca gcgggcgagc
     5221 agttgaagca gcgggtgatg ttgacgttct gctgctggat aaaaccggca ccatcaccct
     5281 cggtaaccgt caggcgtcag agtttatccc cgcacaaggc gtggaggaaa aagcgctggc
     5341 cgacgccgca caactggctt cgctggctga tgaaacaccg gaaggccgca gtattgtgat
     5401 cctcgccaag cagcgtttta acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt
     5461 tgtaccgttt actgcgcaaa gccggatgag cgggatcaac atcgacaacc gcatgatccg
     5521 taaaggttct gtcgatgcca ttcgccgcca tgttgaggct aacggcggtc acttccctgc
     5581 cgatgttgat caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc tggtggtggt
     5641 ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag gcggtattaa
     5701 agagcgcttc gcccagctgc gtaaaatggg cattaaaacg gtgatgatta ccggcgataa
     5761 ccgcctgact gccgccgcga tagctgcgga agcgggtgtc gatgattttc tcgccgaagc
     5821 aacaccggag gccaagctgg cactgattcg tcagtatcag gcggaaggcc gtttagtggc
     5881 gatgaccggc gatggcacca acgatgctcc ggcgctggca caggccgatg ttgcggtggc
     5941 gatgaactcc ggcacccagg cggcgaaaga agcgggcaat atggtggatc tcgactccaa
     6001 cccgaccaag ttgatcgagg tagtgcacat tggcaaacag atgctgatga cccgtggctc
     6061 gttgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta ttccagcggc
     6121 gttcgcggca acgtatccgc agttaaatgc gctgaacatc atgcgcctgc attcgcccga
     6181 ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt tgattcccct
     6241 ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc gccgtaactt
     6301 atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag tcattgattt
     6361 actgctaacc gtttgcggtc tggtgtgagg tttaccatga gtggattacg tccggcatta
     6421 tcgacatttc tctttctgtt attgattact ggcggcgttt acccgctgct gaccaccgca
     6481 ctggggcaat ggtggtttcc ctggcaggcc aacggttcat tgattcgtga aggtgatacg
     6541 gtgcgcggtt cggcattaat cgggcaaaat tttaccggca acggctattt tcatggtcgc
     6601 ccgtcggcaa cagcagaaat gccctataat ccacaggctt ctggcgggag caacctggcg
     6661 gtcagtaacc cggagctgga taaacagata gccgcacgcg ttgctgcatt acgggctgct
     6721 aacccgaatg ccagcacgaa cgttccggtt gaactggtga cggcgtcagc aagcgggctg
     6781 gacaataaca tcaccccgca agcggcggcc tggcagatcc cacgcgtggc gaaagcgcgt
     6841 aatctcagcg ttgaacagct cacgcaactg atcgcaaaat acagccaaca accgctggtg
     6901 aaatatatcg gccagccggt cgtcaacatt gtcgaactca atctggcact ggataagctt
     6961 gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaag ctgccgcgcc
     7021 gcatcggggg aagctgaaag ttttcttcgg tgcctgcgca ggcgtcggga agacctgggc
     7081 gatgctggcg gaagcccagc gactgcgggc gcaagggctg gatattgtgg ttggcgtggt
     7141 agaaacccac ggtcggaaag ataccgccgc catgctgaaa ggtctggctg ttctgccgtt
     7201 aaaacgccag gcgtaccgtg ggcggcacat cagcgagttt gatctcgatg ccgccctcgc
     7261 ccgccgtccg gcactgatct taatggacga actggcgcac agtaatgcgc caggttcacg
     7321 tcatcctaaa cgctggcagg acattgagga gctgctggaa gctggcattg atgttttcac
     7381 taccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat
     7441 tcaggtgcgg gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg tggtgctggt
     7501 ggacttgccc ccggacgatc tgcgccagcg actgaaagaa ggcaaagtct atattgccgg
     7561 gcaggcggag cgcgccattg aacatttttt ccgcaaaggt aatctgatcg ccctgcgcga
     7621 actggcactg cgccgtactg ccgatcgcgt agatgagcaa atgcgcgcct ggcgggggca
     7681 tcctggcgaa gagaaagtct ggcacacacg cgacgcgatc ctattatgca tcgggcataa
     7741 caccggcagt gagaaactgg tccgcgcggc ggcgcggttg gcctcgcgcc tgggcagtgt
     7801 ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg
     7861 ggcaattctc agcgccttac gtctggcgca ggagctgggc gcggagacgg caacactttc
     7921 tgatccagcg gaagaaaaag cagtagtgcg ttatgcccgt gaacataatc tcggcaagat
     7981 tattctcggt cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact
     8041 ggcgcgtatc gcccccgatc tcgatcaggt gctggtcgca cttgatgccc cccccgcccg
     8101 cacgcttaat aacgcgccgg atagccgctc ttttaaagac aagtggcggg tacagattca
     8161 gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc ttaattgcca tgcagtggct
     8221 gatggcgttt gatgccgcca acctggtgat gctgtatctg cttggcgtcg tggtggtggc
     8281 actattttac ggacgctggc cttcggtggt tgccaccgtc attaatgtgg tgagtttcga
     8341 tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat atctgctgac
     8401 cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgctg gcgtgcgtta
     8461 tcaggcgcgg gtagcccgtt accgcgaaca acgcacacgg cacttatatg aaatgtccaa
     8521 agctctggct gtgggccgta gttcgcagga tatcgccgcc accagcgaac agtttattgc
     8581 ctccacgttt catgcccgta gccaggtgtt gctgcctgat gacaacggta aattgcagcc
     8641 tttaacacat ccgcaaggaa tgacgccgtg ggacgatgcc atcgcgcagt ggagttttga
     8701 taaaggcctg cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt accagatttt
     8761 gccgctaaaa agcggcgaga aaacctacgg actggtggtg gtggaaccgg ggaatctgcg
     8821 ccagttgatg atcccggaac agcagcgcct gctggaaaca tttacgttgt tagtcgccaa
     8881 tgcccttgag cggctgacac tgaccgccag cgaagaacag gcgcggatgg caagcgaacg
     8941 tgaacagatc cgcaacgccc tgctggcggc actttcgcat gatttacgca cgccgcttac
     9001 ggtgctgttc ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag gatcgcccca
     9061 cgctcgccag gccagcgaga ttcgtcagca tgtgctgaac accacccgac tggtgaataa
     9121 tctactggat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag agtggttaac
     9181 gctggaagaa gtggtcggca gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat
     9241 caatctttct ctgccagagc cgctgacctt aatccacgtt gacgggccac tctttgaacg
     9301 ggtgctgatt aacctgctgg agaacgcggt gaaatatgcg ggggcgcagg ccgaaattgg
     9361 tatcaatgcc cacgttgagg gcgaaaatct acaactggat gtctgggata acggccccgg
     9421 tcttccacca ggccaggagc agacgatatt tgataagttt gctcgcggga ataaagagtc
     9481 ggcggtaccg ggtgtagggc ttgggctggc aatttgtcgg gcgatagtgg atgtacacgg
     9541 cggcaccatt accgcattca accgaccgga aggtggtgcc tgttttcgtg ttacacttcc
     9601 ccagcaaaca gcccctgaac ttgaagaatt tcatgaggat atgtgacaaa cgttctgatt
     9661 gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg cgacggtatg
     9721 cgcgtctttg aggccgaaac gctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca
     9781 gatttgatta ttctcgatct cggcctgccc gacggtgatg ggattgagtt tatccgcgac
     9841 ctgcgccagt ggagcgcggt gccggtgatt gtgctttccg cacgcagcga agagagcgat
     9901 aaaatcgccg cgctggatgc cggagcggat gattatctga gtaagccgtt tggcattggc
     9961 gaattgcagg cccgtctgcg cgtcgcatta cgccgccact ccgccaccac cacgcccgat
    10021 ccgctggtga aattttctga tgttaccgtc gatttagccg cccgcgttat ccaccgcggc
    10081 gaggaagagg tgcatctcac accgattgag ttccgcctgc tggccgtgct gctcaacaac
    10141 gccggaaaag tgctcaccca gcgccagtta ctcaatcagg tgtggggacc aaacgcggtc
    10201 gaacacagcc actatttgcg tatttatatg gggcatctgc gacaaaaact ggagcaagat
    10261 cccgcccgcc cccgccattt tattact
//
LOCUS       DADTFG010000161        10273 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.162, whole genome shotgun sequence.
ACCESSION   DADTFG010000161 DADTFG010000000
VERSION     DADTFG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.162"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..1821
                     /locus_tag="J1138_003944"
     CDS             <1..1821
                     /locus_tag="J1138_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA8455678.1"
                     /translation="GYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAI
                     VDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRL
                     TVAADDGSERLVSTARTAETTYRFTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAP
                     AAPSQIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYW
                     IAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLNFFKGEIGKTHLAQE
                     LWTQIDNGQLAPDLAEIRTSITGVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLN
                     SMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAMINPANGNTKPMFVG
                     QGDQIFMNEVFLKYLTAPTITSGGNPPTFSLTPDGRLSAKNADISGNVNANSGTLNNV
                     TINQNCRILGKLSANQIEGDIVKTVGKAFPRNGSYASGTITVTVYDDQAFDRQIVVPP
                     VLFRGGKHENFNSNNQQSYWYSTCKLQVLKNGQEIFQQPATDVSRVFSSVIDMPAGHG
                     HVTLTFNVSSYGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            1880..3940
                     /locus_tag="J1138_003945"
     CDS             1880..3940
                     /locus_tag="J1138_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA8455679.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGAITVYEDSKPGTLNDFLGAATEDDVRPEA
                     LYRFEKMVEEVARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAAGATKA
                     GQKKDEALSAATRAEKAADRAEVAAEVTAEPYANIVPPLPDVWIPFNDSLDMIAGFSP
                     GYKKIAIGDDVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQR
                     TNYMLNSESPASWGKSSNMDVPETGTDSFGFTYGKFVCNDSLVGQTSAINMASIAATK
                     SVDVSGDNKYVTTSCRFKTERQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGA
                     AGRITARVRKDKTTGWIFAEATIQAIDGELKIGSQIQYSPEQGGATVSGDYIYLATPQ
                     VENGPCVSSFIISGGSATTRASDLVSIPTRNNLYKLPFTFLLEIHKNWDIAPNAAPRV
                     WDIAAANTGQSAIAAINRGSGKLYMSLSNPSGSYVNSAATDVFAEKTTFGCIAKADGH
                     FHVVTNGKAVNEVYCEYNGVTADKNIRFGGQTNTGERHLFGHIRNFRIWHKELNDRQL
                     KEVV"
     gene            3937..4215
                     /locus_tag="J1138_003946"
     CDS             3937..4215
                     /locus_tag="J1138_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455680.1"
                     /translation="MKDLTLKFHDKLQFKAFLSSLGWAEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCIRNDGYFVNIRMLDDLFDVSVFSDYVVELETPLREWS"
     gene            4228..4521
                     /locus_tag="J1138_003947"
     CDS             4228..4521
                     /locus_tag="J1138_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455681.1"
                     /translation="MDISPLLHALCAVAAQILVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            complement(4613..5470)
                     /gene="sitD"
                     /locus_tag="J1138_003948"
     CDS             complement(4613..5470)
                     /gene="sitD"
                     /locus_tag="J1138_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBA8455682.1"
                     /translation="MMTLLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(5467..6324)
                     /gene="sitC"
                     /locus_tag="J1138_003949"
     CDS             complement(5467..6324)
                     /gene="sitC"
                     /locus_tag="J1138_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBA8455683.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGILANRRR
                     AHKALEDRS"
     gene            complement(6321..7148)
                     /gene="sitB"
                     /locus_tag="J1138_003950"
     CDS             complement(6321..7148)
                     /gene="sitB"
                     /locus_tag="J1138_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBA8455684.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(7148..8038)
                     /gene="sitA"
                     /locus_tag="J1138_003951"
     CDS             complement(7148..8038)
                     /gene="sitA"
                     /locus_tag="J1138_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBA8455685.1"
                     /translation="MLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNARIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            8482..8934
                     /locus_tag="J1138_003952"
     CDS             8482..8934
                     /locus_tag="J1138_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA8455686.1"
                     /translation="MNYFIRPATPDDINKIIEFTTQEAQESEGSVIDLVAVERGVSSA
                     FSENPRSKYWVMEDSSGTVIANTSVVKEWSDFHGNDYWWIQSFYIAPEYRGMGLADEL
                     IKHLIKEAKSEKALELRLYVHGDNGRAIRAYERCGFIESPYTIMKVGL"
     gene            9189..9773
                     /locus_tag="J1138_003953"
     CDS             9189..9773
                     /locus_tag="J1138_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8455687.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(9798..10235)
                     /locus_tag="J1138_003954"
     CDS             complement(9798..10235)
                     /locus_tag="J1138_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA8455688.1"
                     /translation="MLKIRMSRPSEAPEIIQIWKNSVDATHDFLTAHDRQEIEKEVVS
                     FFSETPVLVATNQEDQPLGFMFLHEGHLEALFVDASARGLGVGKHLISHALALHPDLS
                     VDVNEQNHQAVGFYQHMGFKLSGRSERDNQGRPYPLLHLYKAM"
BASE COUNT         2818 a         2361 c         2608 g         2486 t
ORIGIN      
        1 ggatacagcg tgtgggggct gaagctgccg acgctgcgcc agcgcctgtt ccgctgcgtg
       61 agtatccgtg agaacgacga cggcacgtat gccatcaccg ccgtgcagca tgtaccggaa
      121 aaagaagcca tcgtggataa cggggcgcac tttgacggcg accagagcgg cacggtgaat
      181 ggtgtcacgc cgccagcagt gcagcacctg actgccgaag tcaccgcaga cagcggggag
      241 tatcaggtac tggcccgctg ggacacgccg aaggtggtga agggcgtaag tttcatgctt
      301 cgcctgaccg tggcagcgga tgacggcagt gagcggctgg tcagcacagc ccggacggcg
      361 gaaaccacat accgcttcac acaactggcg ctggggaact acaggctgac agtccgggca
      421 gtaaatgcgt gggggcagca gggcgatccg gcatcggtat cgttccggat tgccgcaccg
      481 gcagcgccgt cgcagattga gctgacaccg gggtattttc agataaccgc cacgccgcat
      541 cttgcggttt atgatccgac ggtacagttt gagttctggt tctcggaaaa gcggattgcg
      601 gatatcaggc aggttgaaac cacagcacgc tatcttggca cggcgctgta ctggatagcc
      661 gccagtatca atatcaaacc gggccatgat tattattttt acatccgcag tgtgaacacc
      721 gttggcaaat cggcatttgt ggaggctgtt ggccagccga gtgatgatgc atccggctat
      781 ctgaattttt tcaaaggaga gatagggaaa acccatctgg ctcaggagtt gtggacgcag
      841 attgataacg gtcagcttgc gcctgacctg gctgaaatca ggacgtccat tacgggtgtc
      901 agcaatgaaa tcacgcagac cgtcaataag aaactggaag accagagtgc agcgatccag
      961 cagatacaga aggttcaggt tgatacaaat aataacctga acagcatgtg ggctgtgaag
     1021 ctgcagcaga tgcaggacgg acgcctttat atcgcgggta ttggtgccgg tattgagaat
     1081 actcctgacg gtatgcagag tcaggtgctg ctggcggcgg acaggattgc gatgattaat
     1141 cctgcgaatg gcaacacaaa gccgatgttt gttgggcagg gcgatcagat attcatgaac
     1201 gaagtgttcc tgaaatatct gacggctccc accattacca gcggtggcaa tcccccaacg
     1261 ttttcactga cacctgatgg tcgactttct gcgaaaaatg cggatatcag cggtaacgtg
     1321 aacgcgaact ccgggacgct caacaacgtc acgattaatc agaactgtcg gattctggga
     1381 aaactgtcag ctaaccagat tgaaggtgat attgtcaaaa cggtgggaaa agcctttccg
     1441 agaaatggca gttatgccag cggtacaata acggtcactg tgtacgatga ccaggctttt
     1501 gaccgtcaga tagtagtccc ccccgttctg tttcgtgggg gtaagcatga aaacttcaac
     1561 agcaacaacc aacagtcata ctggtattca acctgtaagc tgcaggtact gaagaacgga
     1621 caggaaatct ttcagcaacc cgcgacggat gtcagcaggg tattttcatc agtcattgat
     1681 atgcctgccg gacacggcca tgtcaccctg actttcaatg tttcttcata tggtgctaat
     1741 aactggacgc caacgaccag tatcagcgac cttcttgttg ttgtgatgaa gaaatctaca
     1801 gccggtatca gtatcagttg aattttataa cccagatacg ggcgccagaa atggcgcctt
     1861 ttttatttgt ggagtgaata tggcagtaca gatttcaggc gtgctgaaag acggtgcagg
     1921 aaaaccgata cagaactgca ccattcagct caaagcaaaa cgtaacagca ccacggttgt
     1981 ggtgaacacg gtggcctcag aaaatccgga tgaagccgga cgttacagca tggatgtcga
     2041 gtatggccag tacagcgtta tcctgctggt tgaaggtttt ccgccttcac atgccggggc
     2101 tatcaccgtg tatgaggatt ctaagccggg gacattgaat gattttctgg gcgctgcaac
     2161 agaagatgat gttcgtccgg aggcactgta tcgttttgaa aagatggtgg aagaggtggc
     2221 acgcaacgct gaagccgcct ctcagagcgc agcggcagca aagaaatcag aaacagcagc
     2281 ggcatcgtcc aggaatgcgg cgaaaacatc agagacgaat gcaggtaaca gcgcgaaagc
     2341 ggcagcttct tcaaaaacag ccgcacaaaa cgcagcaaca gcggcagaac gttcagagac
     2401 aaatgcccgt gcgtcagaag aagcctccgc agacagtgaa gaggcttccc gccgtaatgc
     2461 agagtcagcc gctgaaaatg ccggagtcgc caccacaaaa gcgcgggagg ccgcagcagg
     2521 cgcaacaaag gccgggcaga aaaaggatga ggctctgtcg gcagcgacac gggctgaaaa
     2581 ggcggcagac cgcgcagaag tcgcagcgga agtgactgca gagccctatg cgaatatagt
     2641 gccgccgctg cctgatgtgt ggataccgtt taacgattca ctggatatga ttgcgggttt
     2701 ttctccgggc tataaaaaaa tagctattgg tgacgatgtg gttcaggtcg ccagtgataa
     2761 acaggttaat ttcagtcgcg catcaacggc aacatatatc aacaaatctg gcgaactgaa
     2821 aacggcggaa attaatgagc cgcgatttga gtgtgatggc ctgcttattg agggacaaag
     2881 aacgaactac atgctcaatt cggaaagtcc agccagctgg gggaagtcat caaacatgga
     2941 tgtgcccgaa accgggacgg atagttttgg ttttacttat ggaaagtttg tctgcaacga
     3001 ttctctggtt gggcaaactt cggctattaa tatggcatca attgctgcaa caaagtcagt
     3061 tgatgtctca ggcgataaca agtacgtgac aacctcatgc cgttttaaaa cagaacgaca
     3121 ggtaaggtta cgtatacggt ttgataagta tgatggtagt gcaacaactt ttcttggcga
     3181 tgcgtacatt gatacgcaaa cgcttgaaat taatatgaca ggtggtgctg ccggcagaat
     3241 tacggcacga gtcaggaagg ataagaccac aggctggatt tttgcagagg caacgattca
     3301 ggcaattgat ggtgagttaa aaataggctc tcagatacag tattctcctg agcagggtgg
     3361 ggcaacagta tctggtgact atatttatct tgccacccca caagtagaga atgggccgtg
     3421 tgtatcatca tttattattt caggaggcag cgcaacgaca agagccagtg atttggttag
     3481 tatccccacc agaaataatc tttataagtt accatttact tttttacttg agattcataa
     3541 aaactgggat attgcaccaa acgccgcacc ccgcgtgtgg gatatagcag cagccaatac
     3601 cgggcaatca gcaattgcag caatcaacag aggtagtggt aagttatata tgagtctgtc
     3661 aaacccttca ggctcgtatg ttaatagcgc agcgacagat gtatttgcag agaaaaccac
     3721 atttggatgt attgcaaaag ctgatggtca ctttcatgtg gtgacaaatg gtaaagcggt
     3781 taatgaagtt tattgtgaat ataatggcgt gaccgctgat aaaaatatcc gatttggagg
     3841 gcagacgaat actggagaac gacatctgtt tggccatatt cgcaatttcc gcatatggca
     3901 taaagaatta aatgacaggc aattaaaaga ggtcgtatga aagatttaac tttgaagttt
     3961 catgacaaac tgcagtttaa ggccttcctg tcatctcttg gctgggcgga agatgaagac
     4021 ctccagaata aactgttagt tgatgaaatt ggtttcacct acacagaaac aggggtaaca
     4081 gaagagggag aacctgtctg tatccggaat gatggttatt ttgtcaacat tcgcatgctt
     4141 gatgacttgt ttgatgtttc tgtattctct gattatgtcg tggagctgga aacaccgctt
     4201 cgggaatgga gctgaaagga ggaaataatg gatataagcc ccttacttca tgcactttgt
     4261 gctgtggctg cgcagatact ggttggtctt tttaccggaa actgggctta cggagcgata
     4321 gccggttgta cgttcttcat tgcgcgtgaa catacccagg cagaatatcg ctggattgaa
     4381 atgttcgggc atggcaagcg aatgaatatg ccgtggtggg gcggttttga tccgcgtgct
     4441 tgggatgtgg caagcctgat ggattttgct gtgccggtgg tggcgtgtct gctggtctgg
     4501 ctgttggtta atcgtgggtg aaaaaaggtg agctgtatat gcaacggagg aagaaacccc
     4561 gttgctggaa gccaggaaaa agtatcgggt gttgctgtcc tcggatgttg tgctaattgg
     4621 gtgataacag atcctgtgta tgggagtttc cctttttgcg tgcgttgatg ctggtaacgg
     4681 caaacgtcat gatgaacaaa accgcgaata agacgacaat cgtcggagct ggtgcgctgt
     4741 cgataaaaaa ggacaaccag acaccagtca ttgacactat taccgatatg ccagtcgcca
     4801 gtaggagcgc aatatgaaag cgttgtgtca ggagtaccgc aatggcgcca ggcgcaatga
     4861 gcagagaaat ggagagaatg atacctaccg ctttcagcgt cgccacaatg gtcagagaga
     4921 ccatgcacag cagtccataa tgcagccatc tcgtatgcaa accgcttacc tgcgcctgtt
     4981 gataatcaaa gctgaacagc aaaaaatccc gccattttac gctaataaca agtgtaacca
     5041 gcccggcaat aatcatcgtc tggattatat cgccgatggt tatcccgagc atgtcgccga
     5101 aaagaatatg gtcaagatgt acgtctggtt tgactgcaat atacaagatc agacctgcgg
     5161 caaacatgcc agaaaaaaca atccccatca ctgtatcttg cttgattcgg ctgttgtctt
     5221 tcaggtatcc cgtcgccacc gcgcagaata ctccggcgac gaatgcccct gtagccaaag
     5281 gtaaccccag aatccaggca agcacgatgc caggaaaaac cgcatgactc atcgcgtcac
     5341 ccatcagtgc ccaacctttc aggaccagaa agaccgacag cagcgcacag ggtatggaaa
     5401 ctaccagtga aatcagcagt gcgtgactca taaaggtgaa ctgcagtggc tccagtaata
     5461 atgtcatcat gagcgatcct ccagcgcctt atgcgcacgc cgacgatttg ccaaaatccc
     5521 atgggtgggc gcaaagacaa atgctaaaag aaacagcagt gtctgcgcca ccacgatgat
     5581 tccacctgtc gcgccgtcca gaaagtaact gacccacgcg ccgaggaaac tagttacact
     5641 gccaatagtt acagcaatca tgagcaggcg gggaaaacga tcggttaaca accatgctgt
     5701 tgcaccggga gtcaccacca gacagatcac cagaaaggca ccaaccgttt gcagcgccgc
     5761 aacggtcgaa accgacagca gggtgaagaa gaggagtttt aatctgcccg gatgcaatcc
     5821 aatagcacgg gcgtgattct cgtcgaaaaa ggtcaccata agatctttcc atttgaaaaa
     5881 aagcacgatg atcgagagaa taccaatgat agtgagttgc aggatatccg ccggatcgat
     5941 tgccagaata ttgcccagga cgatggtctg aatgttcaca gatgtcgggt tcaatgacac
     6001 cataaacagc cccaggccaa aaaaggacga gaagatcagg ccgatgatgg catcttcttt
     6061 taagcgggtg cgttggttaa gaaacagcat gctgcccgca gccagtccgc cggaaaagaa
     6121 ggctcccaga gaaaacggaa gccccagcat atatgccccc gcaacacccg ggacaataga
     6181 gtgcgacaac gcatcgccaa tcagcgacca gccttttagc atcagatagc atgacaggaa
     6241 agcgcagaga ccaccgacca tcgccgagac ccacatcgca ttgagcatat attcatagct
     6301 gaagggttcc agtagcacgt tcatctttct tccctctgca ccgctgacgg tcgatgagcc
     6361 acaaaagggc gttcatcgtc agtaataatg ctttcttccg agccgttaag tgtgacatgg
     6421 cgcagtacgc cgctaaaagc cagctctagg ttctcggctg taaaagtggt gtctgtcggc
     6481 ccgcttgcca ggacggtgcc tttgaccatg accgtataat cgcaaaatgt cgtgacggat
     6541 ccgagattgt gtgttgagac aagcatcgtt ttgccttccg cgcgtaactc cctcaacagg
     6601 ctgatgattt tggcttcggt tttcacatca acgccagtaa aaggttcatc cagcaggatc
     6661 acatcaccct gctgtgcaat cgctctcgcc agaaagacgc gtttcttttg tccaccggaa
     6721 agctcgccga tttgtcgatg gcgaaaattc accatatcga cacgttccag cgcatcggtg
     6781 acaatttgac gatccctctt tttggcgata cgcaacatac ccatatgccc atagcggccc
     6841 atcattacca catcttccac cagtacagga aatgaccagt caacctcttc tgactgaggg
     6901 acgtaggcaa ccaggttttt ctgtaacgcc tgtcgcgtgg gaatacccag aacagatatc
     6961 tttccgctgg tcagacgcac aaatcccata atcgctttaa acaacgtcga ttttccggaa
     7021 ccgtttaccc caaccagagc ggcaatagat ccacttggta ctgtgaagga tgcatcgcgc
     7081 agtgctgtgt gcccgttacg ccaggtgaca gtaacatcat taacgacaat gcctgcagat
     7141 tgcatcatta tttctccctt ttcccggctt taattccctg taccagcgta ctggtagtaa
     7201 ccttaagaag gtcgatatac gtcggtaccg ggccgttttc tgtgctcagg gaatcgacat
     7261 agagcacacc gccgtagtgt gcgccggttt cacgcgcaac ctgacgcgct ggtttatcgg
     7321 aaatcgtact ctcgctaaag actgccggga tatgattttt cttaactata tcaacaacct
     7381 tacgtacctg ctgcggtgtt ccttgttgat cggcattaat cggccacaga taaagctctt
     7441 ttagccccaa atcgcgtgcg agataagaaa aagccccttc actggtgacc atccatcgct
     7501 gattctcagg gagttccgta atctgcttac gcaggggggc aagggtttgg gtaatcttgg
     7561 ctttataagt atcggcattg cgttggtagg tttgtgcatt tgccggatcg tattttatca
     7621 acgcatcacg aatattatcg acgtaaatcc gagcattatc tggcgacatc catgcatggg
     7681 ggttaggttt gccctcatag ggtccttcgg tgatccctac aggcgtcaca cccgaagaga
     7741 caattacttc tggaacccca ttgagatgct ggtaaaagcg ttggaaccac aattccagat
     7801 tcataccatt ggcgagaatc agttgtgccc cctgcgcacg tttaatatcg ccaggggtag
     7861 gctgatactc atgaatttct gcaccaggct tggttatgga tgagacttct gcagcatctc
     7921 ccgccacgtt tttggccata tccgcgatga tggtaaatgt tgtaatgacc ttgaattttt
     7981 cagttgcact tgcctgaaat gcgatcgagc aggtgagtgc gaggcccccc aagagcatgg
     8041 ttactttttt tatcgagtgc ataatttagt acctatagtg agcaaaatta gaacaaacat
     8101 agcaacggct atgttttata gcacaaccta tataaaatga aaatgattat cattagcaac
     8161 tttgaggccc aaaacaggga ttaggttgtt tcctgctggc gacgtgttat tgaggaaaaa
     8221 agtaaggcag ctagtaggct ttgaaataac gttctgcggt tggctgatgc aatttcgaga
     8281 gtgcatgtat tgaatgattt ctagccgtta ctgattttcg tattttttgc atgagatgat
     8341 ttgaacgtcc tcgcaccaat cttccatgat tgctcgtcat tgtctctagg actgctatgt
     8401 gccaggagcg gacttttaac aggatttgat gtacggtatt gggcgattcg tatagcagca
     8461 acgaaaatga gagccagact tatgaactac tttattcggc cagctacgcc tgacgatatt
     8521 aataaaatca tcgaattcac cactcaggaa gcgcaagaat cagaaggttc agtgattgac
     8581 cttgttgctg tggagcgtgg tgtatcgtcg gcgttcagtg aaaaccctcg ttcaaaatat
     8641 tgggtcatgg aagattcttc aggaacggta attgccaata cctctgtcgt aaaagaatgg
     8701 agtgatttcc atggtaatga ctactggtgg attcaaagct tttatatcgc cccggagtat
     8761 cgagggatgg gactggctga tgagctcatc aaacatctta taaaagaagc caaatcagaa
     8821 aaggcacttg agcttcgctt atatgtccat ggagataacg ggcgtgcgat tcgtgcttat
     8881 gagcgatgtg gtttcattga atctccgtat accattatga aagtgggttt ataacttccc
     8941 caaatacagc cttcttaatg ctctacctga aatcaggaca caatgccgca agcggcatct
     9001 ttcattcaaa tgatgttctt gaacgttcta ggcacataac gacaacttcc gctcctcgct
     9061 cataacgggc gttcatttat ggcaaaaagg cttaagttta gaaaacatgc gttatagacg
     9121 taaaaaatca cttatttaat cacaaattat gtaatggcat aatcaggttg gtgaaagaga
     9181 gggcgtaaat ggataaaaga gctaaaaatc agatagtcga tagcgatatt gcacgtttgt
     9241 tactcaagct cagaaagtct cgaaacttga ctgttacaga gcttgcccaa cgatctggtg
     9301 tcagtcaggc gatgatcagc aaagttgaac gaggcacctc aagccccagt gcaacaatac
     9361 ttagtcggtt agctaatgca ctgaatatta cgctatcaaa gctttttgca gagcttgaga
     9421 tgcaacagaa ttcacttgta cttttggctg accagcaaca acactggatt gatgaagaaa
     9481 caggcattac ccgctggtct ttgtccccag cgggtgcatg tccggaacta ataaaagttg
     9541 aaatcccccc tatggggcaa ttgactatcc ctgcctctgc aaatgagcat ctttctggac
     9601 aaactctatg gatgttaagc ggcagccttg attttcgagt caatcatcag actcatcatt
     9661 tacaagccgg tgattgtctt gctcttacct ttgccagcga gtaccaaatg agaaacccag
     9721 atagcggcca gagttgttct tacattgtcg ctttcagtca aaaaaatcca tgatgtaatt
     9781 ctcctaaaat aatcatgtca cattgcctta taaagatgta atagagggta gggtctgcct
     9841 tgattgtccc gctcggaacg tcctgaaagt ttaaagccca tatgttgata aaaacctaca
     9901 gcttgatgat tttgttcatt tacatcaacg cttagatcag gatgtagagc cagcgcatgt
     9961 gagatcagat gtttcccgac gccaagtccg cgagctgacg catcaacaaa aagagcctct
    10021 aaatgtccct catgcaaaaa catgaatcct agtggctgat cttcctggtt tgttgctacc
    10081 agcacagggg tttctgaaaa aaagctgaca acttcttttt caatctcctg ccggtcatgg
    10141 gctgtaagaa aatcgtgtgt ggcatcaacg gaatttttcc aaatttggat aatttctggt
    10201 gcttcagatg gtcgcgacat tctgattttt aacatgttat ctcccttaaa gtggtcgttg
    10261 cttgtgtctt gag
//
LOCUS       DADTFG010000162        10151 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.163, whole genome shotgun sequence.
ACCESSION   DADTFG010000162 DADTFG010000000
VERSION     DADTFG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.163"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            173..325
                     /gene="hokA"
                     /locus_tag="J1138_003955"
     CDS             173..325
                     /gene="hokA"
                     /locus_tag="J1138_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBA8455689.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(513..725)
                     /gene="cspA"
                     /locus_tag="J1138_003956"
     CDS             complement(513..725)
                     /gene="cspA"
                     /locus_tag="J1138_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBA8455690.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1006..1296)
                     /locus_tag="J1138_003957"
     CDS             complement(1006..1296)
                     /locus_tag="J1138_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBA8455691.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1730..2440
                     /locus_tag="J1138_003958"
     CDS             1730..2440
                     /locus_tag="J1138_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBA8455692.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2490..3464)
                     /gene="ghrB"
                     /locus_tag="J1138_003959"
     CDS             complement(2490..3464)
                     /gene="ghrB"
                     /locus_tag="J1138_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBA8455693.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3568..4227)
                     /locus_tag="J1138_003960"
     CDS             complement(3568..4227)
                     /locus_tag="J1138_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBA8455694.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     EDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4380..6713
                     /locus_tag="J1138_003961"
     CDS             4380..6713
                     /locus_tag="J1138_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HBA8455695.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFADLSERWQKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGSAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPEDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLIAGGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYTGPELTLTAFEPPASS"
     gene            complement(6682..7122)
                     /locus_tag="J1138_003962"
     CDS             complement(6682..7122)
                     /locus_tag="J1138_003962"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA8455696.1"
                     /translation="MIREVQRSELPAILQLWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7119..7682)
                     /gene="tag"
                     /locus_tag="J1138_003963"
     CDS             complement(7119..7682)
                     /gene="tag"
                     /locus_tag="J1138_003963"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBA8455697.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDIERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7840..8538
                     /locus_tag="J1138_003964"
     CDS             7840..8538
                     /locus_tag="J1138_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA8455698.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAAFHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8767..9969
                     /locus_tag="J1138_003965"
     CDS             8767..9969
                     /locus_tag="J1138_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8455699.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2426 a         2426 c         2600 g         2699 t
ORIGIN      
        1 acttattaag ctagaatagc tgcgggtgct tgagactgtt tgtctcaggt attcaccgaa
       61 aggcagacag agaaaagccc cacctgacta taaatcaaag tgaggcccct ctatgcatag
      121 acaccataag gttagcctct tactcgttgg aaatcaacac agggggctgg gaatgccgca
      181 gaaatataga ttactttctt taatagtgat ttgtttcacg cttttatttt tcacctggat
      241 gataagagat tcactgtgtg aattgcatat taaacaggag agttatgagc tggcggcgtt
      301 tttagcctgc aaattgaaag agtaagcgtc ttcggcggga aattattccc gcctttctta
      361 cggtgttgcg cattctcatt gcacccaaat ttattcttca caaaaataac actagattct
      421 attacgcgat cgattattta tttcctgaaa aacaaataaa aaaatccccg ccaaatggca
      481 gggatcttag attctgtgct tttaagcaga gattacaggc tggttacgtt accagctgcc
      541 gggcctttag cgccgctttc gatggtgaag gacactttct gaccttcgtc cagagatttg
      601 taaccatcgt tctggatagc agagaagtgt acgaacacgt ctttagagcc atcgtcagga
      661 gtgatgaagc cgaagccttt gtcagcgttg aaccatttta cgataccagt cattttaccg
      721 gacatagtgt attaccttta ataattaatt gtgcctttcg gcgatatggc gtgctttaca
      781 gattttgaag cgttaaagga atgtgcacta cgaggggtat caacgataac tcttgaaggg
      841 acttgcctta ctacactgct ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc
      901 acgcattggt gggtgatgca acaattattt ttcatattta tgattaatcg gttggaaaac
      961 ggtcctgtta tcaggaccgt aaacagaaat aaagtggata aaagtctatt ccatcaactg
     1021 cttacttaat gccgggttgg cttgaatcaa acgcatcaat tttagttcgg cacttgaagg
     1081 cttcacgcgt ctggattccc attcctttac catggcgact gatacgccca aaacccgggc
     1141 aaaatcatcg atttttaatc ctgtcccttt tcgtaactgc tcaatttcgg tacaggacgt
     1201 tgttctgtgc gtcagggtaa ttttctgcgt ttcatcttta aaaacaatct gttccaggct
     1261 gctcaacagc tcatgcattg gatctttata ttccattgaa aactcctcaa atcacactgc
     1321 gggatcgtga attgcatcga agctcattaa gaatagtcgg gaaaaacaga cacagcgggg
     1381 tgtagtcagg gcatactctg actatgattc gattcagcca gtcatttagc gtgacttata
     1441 gccacctaac gatctgataa atgccgatat atctcttacc actcccggtt tgtaaagatt
     1501 tcgaaaatct aacgttctgc gaaggcgtaa atctctggct acaaaaaagg caacggcaat
     1561 atcatttttt aacaatgaaa caacaaatta accttgtttc attttctcca tgattttacc
     1621 aggaagatta cgtagaaagg gtatcctgca agcctgtcct ggactatcct tgtcacgtca
     1681 gacacgcgtg tgtcgttgtg cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg
     1741 aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag
     1801 tgggtgtttt gataaagaag gcgatcagcg taaagcgttt atcgacttcc tgcagaatac
     1861 agtgatgcgt agcggtgaac gtctaccaac cctgactgcc gatcagaaaa aacagtttgg
     1921 tccttttgtc tctgattacg cgattctgta tggttattct cagcaggtga atcaggcgat
     1981 ggattccggt ctgcgcccgg ttgtagacag cgttaacgca attcgcgtac cgcaggatta
     2041 cgttacgcaa agcggtccgc tgcgtgaaat gaacggttcg ctgggtgtgc tggcgcaaca
     2101 gctgcaaaat gcgaagttgc aggccgatgc tgcgcactct gcgttaaaac agagtgatga
     2161 cctgaaaccg gtctttgatc aggcattcac caaagtggta acgacgccag ctgatgcatt
     2221 gcaaccgttg attccggcgg cacaaacttt cacgcaacaa ctggtgatgg ttggagacta
     2281 cattgctcag caaggtactc aggtgagctt tgtcgcaaat ggcatccagt tcccgacttc
     2341 acagcaggca agtgaatata acaaactgat tgcgccatta ccggcacaac atcaggcgtt
     2401 taatcaggcc tggactacag cagttactgc aacccaataa agagtaaaag cccgagcgat
     2461 tatcgggctt tactccacgc agtcgcggct tagtccgcga cgtgcggatt cacacagttc
     2521 ttctcaacct ttccttgtaa cgcatcaatc aaattatcca cggcacaggc ggccatgcca
     2581 taacgcgtct catgagtggc agatccaata tgcggtactg cgacgacgtt ggccattgag
     2641 agcaacggcg aatctacgga cagtggctct tgttcgaaga catccagccc ggcggcgtga
     2701 atttcccctt tctgcaatgc agcaatcagt gcattttcat caaccaccgg gccacgtccg
     2761 gcattaatga aaatggcgga ggatttcatt ttggcgaatt gttctgcgcc aaacagatga
     2821 tgcgtctcat ccgttaacgg caggatcagg caaacgaaat ctgactcttg taacagagta
     2881 tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg
     2941 ttatagagga tgggcatgtt gaaaccaaag tgcgcacgtt gtgccagcgc cataccgatc
     3001 cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt cagtgccgta ccagtccggg
     3061 cctatgctcg cggtccattc gcctgctttt acccgttctg ctacctccac aacccgacga
     3121 gcggtagaca acaccagcgc catcagcgta tcggcgacgg tttctgttaa tacggttggt
     3181 gtgtgcatca gcagaatttt tcgggcggta agcgcatcga catcaaaatt gtcatagccg
     3241 acggagatcg ttgatgtggc acgcagtttc ggcatttttt ccagcaatgc ggcatcaaca
     3301 ttctcatttg aacccagcaa gccttcagct tcggcaaaaa ttgctgcatt ttgttcgacg
     3361 gtttgtgggc tgaggtttgc cacctggtga acggtgaaat gttcttgcag gcgttgcagt
     3421 aaatcatcag gtaaggcttt gtagaggata acggacggct tcatgcttct ctccattcac
     3481 tgataaggcg atcaccttag cctgaaatat gatgaaggaa aactgaagaa tgccaggtga
     3541 tttctgcatc acctgacatg aaagggatta cagtgggctt aaggtaattt ctacacggcg
     3601 gttttgcgct ttaccttctg cggtgctgtt gctggcgatt gggttagccg ggccaaggcc
     3661 ttgagtacgg atgcggctgg cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc
     3721 cgcacgctgc tgggagagac gcatgttcag gtcgtgactc cccgtgctgt cggtataacc
     3781 aatcacgtta accgccgttt tcggatactc tttcagtacc attgccacgc cggtcagggt
     3841 gttagcgccc gccggtttca gggttgcgct gctgctgtcg aaggtcacat tgttcggcat
     3901 attgaggata atgttatcct cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc
     3961 acgcagcttc gcttcctgca catccatgta ataaccaacg ccgccgccca gagctgcgcc
     4021 tgctgctgcg ccaatgagcg cgcctttacc gcgatctttc ttcgaagaag agagcgcacc
     4081 aatacccgcg ccaacgagag agcccagacc tgcgccgata gcagatttac ctgcttcgcg
     4141 ttcgccggtg taagggttag ttgtacagcc agataccgcc agagcgccac tcactacggc
     4201 ggcaataaga taaacacgtt tcttcattgt taatccttaa taaccttttt attctttgcc
     4261 acgggttccg tggcgggaga ttatgccgcg tgagcatgaa gattattcct gggaatactc
     4321 ggaaatttgt aagtaatatt taactgctca atacatctaa cctttcagga gtcttcggtt
     4381 tggccaactc atcctcacga tattccgttc tgactgccgc ccactggggg cctatgctgg
     4441 ttgaaaccga cggcgaaacc gtgtttagct cgcgtggcgc gttagccaca ggaatggaaa
     4501 actccttgca gagcgcggtt cgcgaccagg ttcacagcaa tacgcgggtg cgatttccaa
     4561 tggtgcgcaa aggctttctt gcgtcaccgg aaaatccgca aggcattcgt gggcaggatg
     4621 aatttgttcg cgtgagttgg gatgaggcgc tggagcttat tcaccatcag cataaacgca
     4681 ttcgtgaggc ttatggtccg gcatcgattt ttgctggttc ctacggctgg cgttcaaacg
     4741 gcgtgctgca taaggcctcg acattattac aacgctatat ggcgctggca ggcggttata
     4801 ccgggcatct gggggattat tcgaccggcg cggcacaggc gatcatgccg tatgtcgtgg
     4861 gtggcagcga agtttatcaa cagcaaacca gttggccgct ggtgctggaa catagcgatg
     4921 tcgtggtgct gtggagtgct aacccactca atacgctgaa aattgcgtgg aatgcatccg
     4981 atgagcaggg gctttcttac ttttccgcac tgcgtgacag cgggaaaaag ctgatctgca
     5041 ttgatccaat gcgatcggaa accatcgatt tctttggcga taaaatggag tgggtggcac
     5101 cacacatggg caccgatgtt gcgttgatgc tggggatcgc ccatacgctg gtggaaaatg
     5161 gttggcacga cgaagcgttt ctggcgcgtt gcaccacagg ttatgccgtc ttcgcctctt
     5221 atttgctggg cgagagtgac ggtatagcga aaaacgccga atgggcggca gagatttgtg
     5281 gtgttggcgc agcgaaaatc cgcgagctgg cggctatttt ccaccaaaat acaaccatgc
     5341 tgatggcagg ctggggaatg cagcgccaac agtttggtga gcaaaaacac tggatgctcg
     5401 tcacgctggc ggcaatgttg gggcaaatcg gcacacccgg cggcggtttt ggtctttctt
     5461 accattttgc caatggtggt aaccccacgc gccgcgctgc ggtgctctct tccatgcagg
     5521 gcagcttgcc gggtggcacc gatgcggtag ataaaatccc tgtcgcccgc attgttgaag
     5581 cactggaaaa ccctggtggc gcatatcaac ataacggtat ggaccgacat ttcccggata
     5641 ttcgttttat ctggtgggcg ggtggtgcca actttactca tcatcaggat accaatcgcc
     5701 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc cgaatgcttc tggacggctg
     5761 cggcgaaaca cgcggatatc gttctgcctg cgaccacctc ttttgagcgt aatgatctca
     5821 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     5881 gcgatgaagc gcgtaatgat tttgatgttt ttgccgactt gagtgaacgt tggcagaagg
     5941 gcggttatgc acgttttacg gaaggaaaaa gtgagctgca atggctggaa acgttttata
     6001 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     6061 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagtgagcgg tttattcgct
     6121 tcgccgattt tcgccgcgat ccgcaggcgc atccattaaa aaccgccagc ggtaagattg
     6181 aaatcttctc gcagcgtatt gccgattacg cttatccgga ttgcccgggg catccaatgt
     6241 ggctggcgcc ggacgaatgg cagggcagtg ccgaaccgga acagttgcag gtactttctg
     6301 ctcatccggc gcatcgtctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     6361 cggtggcaaa tcgtgagcct gtcaccattc atcctgaaga tgcccagacg cgcggcatac
     6421 aagatggcga tactgttcgg ctgtggaact cacgcgggca aattcttgcc ggcgcggtca
     6481 ttagcgaggg aattaaacct ggcgtgattt gtattcacga aggcgcatgg ccggatttgg
     6541 atttaatcgc tggcggcatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     6601 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     6661 acaccggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     6721 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcatggta
     6781 gaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatggcgctg
     6841 ctgcacatac tgcattagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     6901 cattgctgcg aggaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcttc
     6961 ttcccagacc cagttttgcg cgttggcaag atacgcatcc cgcaccagcg gaatgcagtc
     7021 acgccagtaa ctcgatttta taaagggatg cccccaggtt gtactttcca gccacagttg
     7081 gaggatcgcg gggagttctg aacgttgcac ttcccgaatc atggtttatt tcccggatgg
     7141 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     7201 attgtggtac cgacaaactt aaaaccacgt ttttttagtg ccttagatag ggcgtcggag
     7261 gcgggtgtag atgtggggat ttcgctcaac gttgtggctt gtgtcacctg tggttgatga
     7321 ttgacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     7381 gcccgcgcat taccaataat tgcctgaatt ttcccacgat ggcggataat cccggcgtcc
     7441 tgtaccagtc tttcgatatc ctcttcctgc attgcggcaa ctttcaccgg atcgaactga
     7501 tgaaagcatg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     7561 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     7621 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     7681 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     7741 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     7801 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     7861 ggtcgctggc agctaagcct gcttgcgagc gtggtaatca gtgccttttt tctcaacaca
     7921 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     7981 tacacctggg atagcgatca tcaacctgat tacaacgata ttttgtcgca acgtattcaa
     8041 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaacccc tgtggatgtg
     8101 accagcagta tgagtatggg ctggaatttt cctttatatg agcaggttac aaccggcccg
     8161 gtcgcggcat ttcattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     8221 actaccacgc tggccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     8281 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     8341 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     8401 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgctgcctat
     8461 gctgcgctat ctcaggcaga aaataccact aacaatagcg actatctgta tacgatgggg
     8521 gttagcgcca gattttaacg ttatagatac aattgaaacc cataaataac aactaatgtg
     8581 gcgatagtgg ggactaactt actaaataat aaatttggcg aataattgtc gagtcattca
     8641 ttcctgaact aatgcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     8701 ataaagcgca cttcgttatg gttgtcagca gagatttttc ctttttatta ctgcaggaat
     8761 actgccatga caccttcaaa ttatcagcgt actcgctggc tgacactcat cggtactatc
     8821 attacccagt ttgctctggg gtcggtttat acctggagtc tgtttaacgg tgcgctttct
     8881 gccaagctgg gggctcctgt aagccaggtt gctttctctt tcggcttgtt aagtctgggg
     8941 ctggcaattt cgtcttctgt tgcgggcaaa ttgcaggaac gttttggtgt taaacgcgtc
     9001 actatggctt ccggcatttt gctgggatta ggtttcttcc tgacagcaca ttccaacaac
     9061 ctgatgatgc tgtggttaag cgccggtgtg ttggttggtc tggcggatgg tgcgggttac
     9121 ctgttgacgc tctctaactg cgtgaagtgg tttccggagc gtaagggact tatctctgct
     9181 tttgctatcg gttcttatgg tctgggcagc ctgggtttta aatttatcga cacgcacctg
     9241 ctcgaaacgg tgggtctgga aaaaaccttt gttatttggg gcgcgattgt actggtgatg
     9301 attgtctttg gcgcaacgtt aatgaaagat gcgccgaagc aggaagtgaa aaccagcaat
     9361 ggtgtggtgg agaaggacta caccctggca gagtcgatgc gtaaaccgca gtactggatg
     9421 ttagcggtta tgttcctgac tgcgtgcatg agtggtctgt atgtgattgg tgtagcgaaa
     9481 gatatcgctc aaagtctggc gcatcttgat gcaatttccg cagccaatgc tgtgacggtt
     9541 atttccatcg ccaacctttc tggtcgtctg gtgctgggca ttctgtctga caaaatcgcc
     9601 cgtatccgtg ttatcaccat tggtcaggtg attgcgctgg tgggtatggc ggccctgctg
     9661 tttgcaccat tgaatgcagt gacgttcttt gcagcgattg cctgtgtggc atttaacttt
     9721 ggtggcacga ttacggtgtt cccgtcactg gtcagtgagt tcttcggcct caataacctg
     9781 gcgaaaaact acggcgtcat ttacctcggt ttcggtatcg gcagcatttg tggttcgatt
     9841 atcgcttcac tgtttggcgg attctatgtg actttctacg tgattttcgc cctgctgatt
     9901 ctgtcactgg cgctttctac gaccattcgc cagccagagc agaaaatgtt gcgtgaagca
     9961 catatttaga aatatgttgc tgctgaaata aatacgatga gggagacttc gaagtctccc
    10021 tttttttatg gttgcatgtt aaaagaacaa tggcatccaa aaaatgaata tttttcgttt
    10081 atacattctt tatgtaaata taaatatttt cttaaaaaaa cggtaattaa attgcgtttt
    10141 atgtgtaatt g
//
LOCUS       DADTFG010000163        10029 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.164, whole genome shotgun sequence.
ACCESSION   DADTFG010000163 DADTFG010000000
VERSION     DADTFG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.164"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            19..1599
                     /locus_tag="J1138_003966"
     CDS             19..1599
                     /locus_tag="J1138_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA8455700.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESNPVTLNVHEDTAIADIPTPDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIY"
     gene            1599..2774
                     /locus_tag="J1138_003967"
     CDS             1599..2774
                     /locus_tag="J1138_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HBA8455701.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IAYNECEGWLKSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFD
                     EFYSRLIHEGKVGIMAGHVYGTEGEGYLRLNLACGREKLYMGLTRLVSVIKNINQGE"
     gene            2777..3373
                     /locus_tag="J1138_003968"
     CDS             2777..3373
                     /locus_tag="J1138_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA8455702.1"
                     /translation="MNNTDLIYLIKHFMHNELKAVEEVIYSPLSEFANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3445..4392
                     /locus_tag="J1138_003969"
     CDS             3445..4392
                     /locus_tag="J1138_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBA8455703.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDGYQADTLSLLPGVRIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSGLENFISH
                     KYLSMMRNGALIINVARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(4692..6068)
                     /locus_tag="J1138_003970"
     CDS             complement(4692..6068)
                     /locus_tag="J1138_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein VasL"
                     /protein_id="HBA8455704.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDL
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PLSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKMAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(6068..6496)
                     /gene="tssE"
                     /locus_tag="J1138_003971"
     CDS             complement(6068..6496)
                     /gene="tssE"
                     /locus_tag="J1138_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBA8455705.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQISERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(6500..7036)
                     /gene="tssJ"
                     /locus_tag="J1138_003972"
     CDS             complement(6500..7036)
                     /gene="tssJ"
                     /locus_tag="J1138_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBA8455706.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(7017..8096)
                     /gene="tssG"
                     /locus_tag="J1138_003973"
     CDS             complement(7017..8096)
                     /gene="tssG"
                     /locus_tag="J1138_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBA8455707.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(8060..9820)
                     /gene="tssF"
                     /locus_tag="J1138_003974"
     CDS             complement(8060..9820)
                     /gene="tssF"
                     /locus_tag="J1138_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBA8455708.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFA
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQDRRMLGAHFS
                     PMGFEDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         2505 a         2380 c         2524 g         2620 t
ORIGIN      
        1 ttatatgaga taacccacat gaaacaaaag aaagcctgga gtttttttca gagcctgggg
       61 aaggcattta tgtatcccat tgctctgcta agtgtatgtg gcatgatgct agggctggga
      121 agtggtttag ccagtgatga tatggcaaag ttaattccat ttctggctat tccaataatt
      181 aaaaccatac ttgatttcat tgttagtctt ggtttgtttg cctttgttaa tttacctgta
      241 ttgtttgcga tagcgattcc cttaggatta ttaaaagata aagaggataa agcctatggt
      301 gctttttctg gcttaattgg ttttatggcg atgcatctgg gaacgaactt ttatcttaaa
      361 cagcacgact tattggtcgt tgctgaccaa atgtcgacac atgggcaaac catcattctg
      421 gggatccagt cctacaatac cagcgtgttg gggggaattg ttgctgggtt attagtcgcc
      481 agcatgtata aaaagatcgt taatttacgc attccagaat cattaggttt ttatagcggc
      541 ccacgtctgg tgcctatcat tacactgatt gtgatgagtg gatttggtct gatcattcct
      601 tttatctggc cgccgttttt caatcttttc atgctcattg gacactggat ttcaacttcc
      661 ggtcctgttg gttatttctt ctatgcagtt gccgaacgtg tgacgattcc ttttggctta
      721 aaccatctgg tgacgtcagt tttccgcttt acgccaatcg gcggttcggc tgtgattggc
      781 ggcgaagaat attacggcac cctgaacatg tttatggcat acgttaaaga gaatgcggtc
      841 attccgctgg atttggcggg gaaaatggag cagggcaaac tgatgattca gtatggtctg
      901 gctggtgccg cgctggcgat gtatcgcact gctcatgctc aaaacagaaa ggctatcaaa
      961 gcattgctta tttccggggt gcttacggtg attattggcg gcgtcagcga accgattgag
     1021 tttctgttct tatttgtcag tccactgctg tttgtcttcc atgcctttat gaatggattc
     1081 gctaacatgg ttctgccata tatgggcgtg aagatgggat ttactggcga tctgattcaa
     1141 tttattagct ttggcgtatt gcgtggcaca agaacaggtt ggccgatcgc ggtgtgtgtc
     1201 gaagtggcct atttcttcat ttattacttt gtgttccgtt ggaccattct taaatttaac
     1261 ctgatgaccg taggccgtga agagtccaat cctgtcacgc tgaacgttca cgaagatacg
     1321 gctatagcgg atatcccaac tcctgataaa tcagagctgc aagcggcgga gcagatggtt
     1381 aaggcacttg gtggtaaaga gaatattaag tcactggata attgcgtaac tcgtttacgt
     1441 ttaacaatcg cagatatggg attgattgac gaagttgcaa taaaaagagc tggcgggatt
     1501 gcggttgtta aacttgatca aaatacccta caagtcatta tcggcactaa agtcatcgcc
     1561 ctgcgtcggg atatggataa ctatatgggg atatactgat ggatgttttt aatactcctg
     1621 tttcccgcaa aggaacatat tgtacccaat gggacttttg cgaggacaga tttggtgtaa
     1681 aagatgtact accattctct atttcagata tggatcttcc gataccagac gctatcacca
     1741 gagcattaaa aaaacgcctg gaacacccga ttttagggta cagtcgttgg caacatggtg
     1801 aatatctgaa tgcgatagta aactggtatt atcaacaata tcaaacagat attaaacctg
     1861 aatggataac ctatagcccc agtgtgatgt actctattgc aaaagcaata gagttattga
     1921 catcgcatgg tgataatatt ctcgttttta cacctgtcta taatgcattt tttgatgtaa
     1981 ttaaacatag cgagcgaaat attctgacag cctctttgat tataaaaaat gaggggggct
     2041 acgctatcaa ctggcaagat tttgatctta agattaaaag cgcaaagatg gtgttattat
     2101 gtaatcctca taacccgaca ggaacagtct ggtcggaaga agaactgcat aaaatcgctg
     2161 caagctgtac aaggcataat gtctggttat gttcagatga aatccatagt gattttgttt
     2221 ttaatcgtag ctttacatcc gcgcttaaaa taaaaaaaga aaaagtagta gtatttaact
     2281 cgatatcgaa aacatttaat gttcctgcac taacaggatc gtatatgatt tcaactgacg
     2341 ataattttaa tcataagttc agaacgatat cgaggtatcg cgattttgta aactccccat
     2401 cagtactgaa tataattgca actattattg cctataacga atgtgaggga tggttaaagt
     2461 cattaaaggc acatattgcc tctaacattc aattcaccca gcagtatctg aatgaaaata
     2521 ttccagaatt gatcgtaagg ccagctgatg gttgctattt ttcctggata gattgttctg
     2581 ctattggtta tccttttgat gaattctaca gtcgattaat tcatgaaggc aaagtgggca
     2641 ttatggcggg gcatgtctat ggaacggagg gggagggcta ccttcgtctg aacctggcct
     2701 gcggtcgaga aaaattatat atggggctta cgcgtcttgt gagtgtaatt aaaaatatta
     2761 atcagggaga ataaaaatga ataacacgga tcttatctat ctcattaaac attttatgca
     2821 taacgaactt aaagcagtag aagaggttat ttattcccca ctttctgaat tcgctaattt
     2881 aattaaagta ttacaatctt gtcagggaaa agttgttttt attggtgttg gtaagtccgg
     2941 tattattgcc agaaaactcg cagcaacttt tgccagtacc ggaactccct cgttttttgt
     3001 tcacggtacg gaagcggtac acggcgacct tggaatggtg gcgaaagacg atgttgttat
     3061 tcttatttca aacagtggtg agacggcgga aatcctggcg acactgccta gtttgaaaaa
     3121 aatgggtaat tatttgatct cttttacccg cagtcatcac tcatcgcttg cgataagttg
     3181 tgatttatct gttgaaatcc ccgtcaaaag tgaggcggat aatttaggtt tagcaccgtc
     3241 atgctcgtcg actgttgttc tggttgttgg tgatgcggtg gcgcttgcac tctctgaatt
     3301 gaaaaaattt actcgtgctg atttcggctt atatcatcca ggaggtgcac tcggcattaa
     3361 agcaaattca taatttcaaa atataaatac agtaaccggt cacgccatct tttggggtgg
     3421 cgttctttga agtgagtgtc cgctatgaaa aatgttctgg tcaccgtccc cagcttttca
     3481 gcacgctgtg tttccgccag caagttgcta agagagaata attttaatct tattattaag
     3541 aataatgtgg agcatctgct taaatcagaa tccactgcat tacgagaatc tatttgcgcg
     3601 gtcatcgccg gaaaagatgg ttatcaggcg gacacgctat cattgctgcc tggtgtgaga
     3661 ataatatcga gattcgggac ggggatagac aatattgatc ttcgtgcggc acagcaatcc
     3721 ggtattgttg tcaataatgc ggttgggatt aattcaaatg cggttgcgga atttatcatt
     3781 ggacttatct tcgcgagcat gagaaatatc cctggcagct atcatgcgat gcaaaatggc
     3841 tactggggtg agtcgcatgg ctgtgaatta caagggaaac ggattggcct ggttggctac
     3901 ggtaatatag gtaaaactct ggcgaaaagg ttatccggtt ttgatgttga gcttttagct
     3961 tttgacaaac aacccgacta tcaggttgct gacaaagctg gagttcagtt tgtatcaatt
     4021 gaagatatct ttatgcagtc gcatgtcatc attgttcttt tgcctttttc ttctgggctg
     4081 gagaacttca ttagtcataa atatttgtcg atgatgcgca atggtgctct gatcatcaat
     4141 gtcgcaaggg gaaagttgct ggatgagggc gctttacttc aggtgattga agaacgaaat
     4201 gtgtttgcgg cgctggatgt gtttagctct gaaccgttgg cgcaatttag cccactgcta
     4261 catgccaaaa atatcattac aacgccgcat atcgcggccg ccaccgttga gtcataccag
     4321 caaaccggga tacacgttgc ccagtcgata attgattact tcgcaggaag ggagataaaa
     4381 aacgtgttgt gagttcagcg aacaaagatt ttaaatacaa aaaaaccgca atttgtagaa
     4441 cacattgcgg tttgtcagca ttctaataaa ttggttgcgg gggccacagt caagaggtta
     4501 atccaatctt ttgaaactta aggtattcac tgttcccgaa cccaagctat ctaccgaaat
     4561 atacactcat tatctgatgt gaatcaacga gtagatcgaa tggcctgttc ctctcttttt
     4621 ttgtacacag agactccttt aaaaaatggc aaggtcacat atcccttatt ggtacataaa
     4681 tcccccgatg tttactgact ttgttccacc ttcgtatcca gtaaaatgac atagcgattc
     4741 aacaactgat ttaaccgggt atcaatttgc gtcaacagcg ttggcgaaac ctctccggtc
     4801 tgtttttgct cttccagttg ccgaagcagt tcttccagcg gcggctcctt taacgactgc
     4861 cggatgccgt acactgccat ttttagttcc gaaccggtca gatatctccc cttacgttca
     4921 tccagtgcat ccagctgcgc cgacaggcgc tgtagctggc tctgcgcctg agcatagcca
     4981 ttcagtgcct cagcagacag ggcgctcagc tcccgctgtt tgcgccactg cgcgttcagc
     5041 gccctgacct gaggattatc cggccacagg gcatcaagct ggcgtagctg gcgatatccc
     5101 tgctccagtg gccagcgggc aggcaatttg tttaactgct ccagttgcat ctgacttgcc
     5161 gcaattgttc tgtcctgagc cagcagcgcc ttatccttta catccagctt tgccagttcg
     5221 ccgctctcca gcgacagcgg caacggcatg acagacagtt gtattcgctg gattagcgcg
     5281 tccggctgat taagctgcga ccagccccat aatcctgttc cgccgagacc ggccatcaca
     5341 accattccgg ccataaatat cggccatgtc cgctgccggg agatttcagg ggatggaacg
     5401 ggtggactgg catccttatc gttttgctga acggatctgg ttgcaaaaac cggctgtgga
     5461 ctttcctctg actttttcgg ttgattcatt tcccggcggg gtaattcagg gggagtgata
     5521 gtggtttccg ctccctgagg cgccagccgt tccagacgca atgcggtatt gcgaaactgc
     5581 ccggccagta gatccagttt cgtcatatgc cacagttcac actgttgcag cgtggtttcg
     5641 atggcggaaa gatgctgtac acaacggtag atctgcggca gatcctgata ctgaatatcc
     5701 agcgttctca gtgcctgctg cattttttct gtgacccagc tgagcagtga tattctggaa
     5761 tgtacctgta ccggccagaa gtcagcccag cgctggagca gtatatccag tgaaccgagt
     5821 ccgtctgcca tccctgccag cccctgccgt ctggttatcg ccagtacgta acagaccagc
     5881 gtctgtagtt ccacgccatt ttgccggaac agcgcgaggc agagtttttc cacctgacgc
     5941 cagtccacat ccggtcgggc cgggtgagtc agtttactca tttcagcctg cagttcaccg
     6001 gattccttat aagtacaggg atcgccgccc atgacgaact ggctgataaa attcgcgtta
     6061 cttgccatca gtacgcccgc tcctgttgtt tgagatgacg cagaacaatt cgtccttccg
     6121 gagaaaatac gccatcaaag cgtatccagc cggtatcggg aagctgcgcg tggatcaggt
     6181 aattaagatg ccccggacgg ggctggggga gtaattccac ctgtatctga ctcaggcgtg
     6241 gttcataacg cagcagtgtt tcttcaatct gacgcatcag gccatgaata cccgcagcca
     6301 tcccctgatg gatcagactg agatccggca gaccgtaatc cggcaaatgc gccagcgtgc
     6361 ctgcccggca gttgataatg cgttgcatat tgtccatcac ggaaaggata agctgatccc
     6421 gctcgctgat ctgttccagc ggtaactcgc cgctgaaact gaacgtcagc atttcatata
     6481 aggaaggtgt cgacatcggt tatttatcct tgagaggcag cagtcgcagg tcgcttctca
     6541 tcagttcaat cgagcgcggc ttgtcggcct ccagttcgtc ccttttgatc accagacgcc
     6601 agctgtcgct tttttcatca gggtgataaa actgcccaat aatggcgaca aactgcgtct
     6661 ctttatccag cggcacattc agggatgccg tgccttccgg ttttacccag acggtgtgtt
     6721 ttgccagtac gtccttctcc agcgtcttct cttcctgcat aaacagggtg tcgtaatccg
     6781 ccttgtcaaa atcttcgcgg gttttcagtt gccagacatg caccatcgtg gaaagcggcg
     6841 tatcttccgc atccgtattc agggcgctgc gggagaagaa atcgagatgc agtattttga
     6901 tttgtttgta gaaaacggct gaggccacgc ttttcgattc atccaccact ttctgaatca
     6961 gcccgcagcc actcagaaca accgtcagca tcgccataca gagcaacgcc ggacgtttaa
     7021 aacgaatagg aaacatgagt catactctcc ctgttacgaa tggaaaaagc gggtattaat
     7081 ccctgataac gccccagact gaccgtcact gttccggtcg cgggcgcggc aaaggaggcg
     7141 cgaaggatgc cggtacggct aatctgtaca cgctgttttc ccagccgggc agcaggcagc
     7201 aggctgacgg gcagcgtcag ttgcagacgg gcctgacagc gccagcccag ataaacgcgc
     7261 agcagcacca gcaaatcgct gtgcagttgc ccgccgggca gccagccacg tgcttctgcg
     7321 gcatcttcgg tatacagttt cagcagcaac tggctgttga cgtcggtacc ggtacgcccc
     7381 agcagcgttc gcgttttcag gctgatgcgt gaattttttg acagacccgc aggggccggg
     7441 agaattacag gctgcggatc gtgaggcacc accgtcgctt tggtcagcgg ggcaagcagc
     7501 cctaccagtg ccgtgacgcc ttccgccgtg cgcgtcggca ggcgcatcac gctgagcagg
     7561 gccagaaaac gggataccgg cgtggcgata tggttctcgc tgccgggaat acccagcccg
     7621 atgagtccca gcagacaacg ggaaatgtca tccacggcac ctgcttcaaa cgacgccggg
     7681 tagttatatt tgcgccagat gcggtagtac tgagtaataa agcgatggtt aaaaatatcg
     7741 agaaacgcca tcactgcgtc atgtccgtca tgacgctggg taatgtagtc cagataggcg
     7801 gttggcagcg gcgaatccac gccgtacagg ccgagaaaag tggtgcgcac cgtggggggc
     7861 gcgtcgggat tgcccacgtc acgttcaacg ttttttaact cgctgaccgg aaagcccata
     7921 ccgggatgag gacgaaagcg caccgcatcg gtggcggggc tgtcggtact gcccagcggc
     7981 gcgttctgct ggctctgttc cagcaactgg caaaaacggt aaaaattgat ccgcgcaata
     8041 tccggggcca gccggtcggt cagcccggta cgcgctggct gtgatgctct gtccattgca
     8101 ggcactttcc tgtgggttgc agaattaagg tcagttgagt gaataagtgg atatcggcat
     8161 acagtgcgaa gaagcggtgc agcagttcac caaatagcgt gatatcacct tctccggcaa
     8221 agccgttact gtccagcgtc acctgaatat ccacgccacg cagcaaaaag cctctttcaa
     8281 aacgctgtat caggctgtgc tgaacgtcga caatcgcctc cagacgacgg cggttcatct
     8341 cgctttcggt ccagtcgtac aatgccagcg ttccgcgcag aatttcggcg ttatccatca
     8401 tcgataaaaa gttagaacca aggtgactga gcacccgcca gtggaagcgg tcacgcgccg
     8461 ggggatagca gggctgcgtc ggagcgcaca ggttgcgcac ccgcaggacg ttctgtgagg
     8521 catgaactgg cgtatcgagc agcgtgctct gtaaagcctt acggggaagc tgcccgttag
     8581 taccggtcag gcttaaagag agcgtttcat cctccagcat ccggtcagta tcaaatgcat
     8641 cgccgcccag aatcagccag gtgtcatgca ggccggaagg gccgcgcttt acccgcgtgt
     8701 ggtaatagcg ttccggcgca tcgtggcgca gcatcccgcc ccggtgacga aagctggaga
     8761 aggggacata cgcctgactg ccggtatggc gcgaagaaat aatgttgtcg acggaataaa
     8821 tctcggtatg cccgtcctga atgcgcatcg ggcgcagcag aaattcgttt tccagtggcg
     8881 aaaggtgtaa cggatcggct tcgagcggga acaggttgat gaccggcacg cagtgcagac
     8941 ggatattatc gctgtcaaac ggcaggtcgt gaggccagtt ctcgttcagc accacgtcaa
     9001 tctcaaagcc ggttatcccc tccggccatg caacctgctc cagcccgttc agcgccacga
     9061 acataaactt ctcgcggaag gtgaaatact ccagcagcaa ctggtaaccg ctgaaggctg
     9121 actcaccttt tggccagagc ctgtcatcat cctcaaagcc catcggggaa aagtgtgcgc
     9181 caagcatccg gcgatcctgc cccggcagac gcagccagaa ctgctgcgtg ccaagcgtta
     9241 aagcccggtg caacgcacag gctaccgggc tgtcggcatt gagatagagc ggcaggcggg
     9301 ataaatcgat ctgcgaccag tcaccaacca gcgggccgca cgcaaagcgc agacggataa
     9361 ccgagcgtcc gtccggttca tattgcaatg acacgtccgg caacgccagc ggatgcagcg
     9421 tgatatcacg cgtggcactg tagacgcagc gggttctgcg ctcgccaaca gggcgcgaca
     9481 atacctgaaa atcgctcagc gtttcgctct gcttcatctg ccggtgatct gtcgacagtt
     9541 caaccacgga aagtgacggg atggttcgca ggtagtgggg ccacagcagg ctgaccagcc
     9601 cttcagtcag ttccggcaga tcgtcgtcca gcttttcgcg caggcggccc attaaaaaag
     9661 cgaagccctc gaacaggcgc tccacatagg gatcgcgtgc gccgggttta tcaagattaa
     9721 gcatcgcagc acggtcgggg taagcctgcg caaattcttt acccgcttca cgcaggtagc
     9781 gcatttcggc ttcatagtag cgttgggtca ggtcgtccat tcagagagtt atttcccatt
     9841 taactaaaaa gttagataac agtaaaaaga aaatatattt tacagttcat aaaacaccag
     9901 taaaaaacaa caataaccca taaagatgag cgcaattaaa aaaccattac aatcatctgt
     9961 taaaaataaa caatgactac tttgcttata gtattgccgg tcagttactt catcttcctg
    10021 ggtattaca
//
LOCUS       DADTFG010000164         9949 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.165, whole genome shotgun sequence.
ACCESSION   DADTFG010000164 DADTFG010000000
VERSION     DADTFG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.165"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            242..1336
                     /locus_tag="J1138_003975"
     CDS             242..1336
                     /locus_tag="J1138_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HBA8455709.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1360..1572)
                     /locus_tag="J1138_003976"
     CDS             complement(1360..1572)
                     /locus_tag="J1138_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBA8455710.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1832..2140
                     /locus_tag="J1138_003977"
     CDS             1832..2140
                     /locus_tag="J1138_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HBA8455711.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2199..3293)
                     /gene="yaiW"
                     /locus_tag="J1138_003978"
     CDS             complement(2199..3293)
                     /gene="yaiW"
                     /locus_tag="J1138_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBA8455712.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3306..4526)
                     /gene="sbmA"
                     /locus_tag="J1138_003979"
     CDS             complement(3306..4526)
                     /gene="sbmA"
                     /locus_tag="J1138_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBA8455713.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4878..6035
                     /gene="ampH"
                     /locus_tag="J1138_003980"
     CDS             4878..6035
                     /gene="ampH"
                     /locus_tag="J1138_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBA8455714.1"
                     /translation="MKRSLLFSAVLYAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6036..6659)
                     /gene="iprA"
                     /locus_tag="J1138_003981"
     CDS             complement(6036..6659)
                     /gene="iprA"
                     /locus_tag="J1138_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBA8455715.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRNGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6745..9732)
                     /locus_tag="J1138_003982"
     CDS             complement(6745..9732)
                     /locus_tag="J1138_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA8455716.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQVDISNSVIHGAITSELPFGYYNITPAEVNAKGEVVTPQTYNGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVACIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSVI
                     DTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATDHLTVDSYSTVNLTEST
                     GWNNYSHLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVVSGVFD
                     INSSDYVLNADLVNDRTWDTAQANYGYGVVAMNSDGHLTINGNGDVDNGTELDNSSVD
                     NVVAATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAE
                     QRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVS
                     YFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVD
                     QDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKA
                     GYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTY
                     SEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTGGSAVRVGLGTQFSFTKNFSAYTD
                     ANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2590 a         2472 c         2334 g         2553 t
ORIGIN      
        1 cttcgttatc ttataattaa gagaaaaaac tcaccacatg gtcatttaag ccaggaataa
       61 attagcttcc cgttcatcat ttttgtgatc ttgcacacat ctttgagata acgcactttc
      121 ttcaccagta cgttgctcgc tttgtcagca gaagattgca acattctgtc agaaggccgc
      181 gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa
      241 gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc
      301 tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg atgttgtgct
      361 gctgggcatt gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa
      421 tgcagacgat cctgcccata ttgcgttgcg tccttcggcg accagccttg cgcaggtgcc
      481 aggtaaacat gagcatcagc ttatcgacgc acaaaacggt cagccgttgc cgacggtgga
      541 tgtcattttc ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct
      601 gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg gcttcagcgg cctgtatgga
      661 taaagatgtc accaaacgtc tgctgcgcga tgccgggctg aacattgcgc catttattac
      721 cctgacgcgc gctaatcgtc acaacatcag ttttgccgaa gtggagtcta aactggggtt
      781 accgctgttt gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag
      841 tgaagaacag tacacaattg ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt
      901 tgagcaaggg atcaaaggtc gtgaaatcga atgcgcagtt cttggcaacg ataacccgca
      961 agcaagcacc tgtggcgaga tcgtactcac cagcgatttc tatgcctacg acaccaagta
     1021 cattgacgaa gatggcgcga aagtggtagt tccagcagcc attgcgccag aaatcaacga
     1081 taagatccgg gcgattgccg ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg
     1141 tgtagacgtg tttttaaccc cagagaacga agtggtgatc aacgagatca acaccctgcc
     1201 tggcttcacc aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac
     1261 cgacctgatc acgcgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcact
     1321 gaaaaccaca atgtaatatt caaacgcccg gtaatattct tattgggcgt tttcagtttc
     1381 ttcatcgcga cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag
     1441 accgacggca taggtctgcc aaccttttgc ttcaaatccc agtaacccca ccacgccatt
     1501 aagaatgaat ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac
     1561 aggcagattc atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac
     1621 tgtctgtggt gtagacaaca ctttcgcaat tgttaataaa tttaacttaa ggttgctatt
     1681 ttgtaactca caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat
     1741 tctgatattg acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca
     1801 gaagagaagc agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct
     1861 gtttagcgga aaatatcgca atgcatcttc aacgcctggc aatattgcct atgcgttgtt
     1921 tgtgctgttt tgcttttggg ctggggcgca attgctgaac ctgctagtgc atgcgcccgg
     1981 cgtctatgag cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt
     2041 aggtgtcggc accatttttg ggctgatccc gtttttagta ggctgcctca tttttgcagt
     2101 agtggcgcta tggctgcact ggcgacatcg ccgccagtaa gatttacgtt gccagatgcg
     2161 gcctaaaacg ccttatctgg cgtgacattc ctgtttgatc actgtttcat acaacgcgcc
     2221 cgccgttcgt ctacgcgctt cgcaaaccag gccgtagtca gattgcgggt gattttcggg
     2281 ctttccagtt gaatgccagg caacatttcg cggggtagtg atttaccggt tttcgcttcg
     2341 gcaagttgat aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcccctttc
     2401 tctaactgac ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt
     2461 gccagttcgg ttttcccggg ctctttactg tcgtaacgaa ttaaatcacc gtccagtgcc
     2521 agcttcacgc cgctggcctt actgaccgcg ttctgaaacg cggcattacg gctggcgtac
     2581 cagccagcgt taaaatcagc aaaacggtat atcggcgcgc tatagctggc gggataattc
     2641 agtaaatggt aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacggact
     2701 gtaccgtcca ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatactgacc
     2761 tgcatcggcc caccggtgcg caccggattg agcgaaccaa acagcgtctg ccccattggc
     2821 accatgctga tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct
     2881 aaccgttcgc tatagctttt gccgtttggc gatttgattt ttagcgcggt atgcaccaga
     2941 aaagcaggaa tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt
     3001 aagcccggaa cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc
     3061 gagcagacgt tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct
     3121 ttcgcccagg catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgcc
     3181 acatctatcg ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct
     3241 aaaaccagaa ccgccaacaa tgacagcgaa gatagaggat tgacgcgcga cattacttct
     3301 cctttttagc tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca
     3361 aagctgcgca gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc
     3421 aggtactgga aagcaccgcg aacctgacca aaaacgttag taatctgcgt catcaggccg
     3481 agtgtaatcg taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg
     3541 acctgcaaat agagaatgcg ggcgatattg aaatacatat agtgaaaata gaggcggaaa
     3601 tagtttttcc gtacggcgct aaaaagctcg cgtaccgtag gcggcgtcgc gcgcgtggca
     3661 tcgtcttcac cataaaccag ctctttacgg taggcagcct ctacacgctg gtttttaaac
     3721 tccagccccg gcagtttgat ccctaccact gccagtaatc cggtccccat cagcgaccag
     3781 acgatagcgg caatcaccag accatacgga atgtgcccga caatcggcag ctccggcaca
     3841 tgtgcggaga gcgttaccag caccggcagg aaggcgatca acgtcatgat agcgttgata
     3901 aagctgacgc ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc
     3961 tgcgcggccc cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc
     4021 gccgtacgcc agcggaacac gtagtgactg acaaagaagt tgttcagcac actgatcacc
     4081 accgcgatca gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatggtg
     4141 actttatgcg gcgaacttag cgccgtttga atcagatcat agaacggcgc ataccaggcg
     4201 ttgacggcaa ccccgacttc caccaaaaac caggtgacga agataatcag cgcagtaccg
     4261 agtattgacc agtattgcca acggtgcggg ctatagataa accagaacaa tgcaaaaagt
     4321 cctacgcaaa caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg
     4381 gcgctaatcg ggatctgccc ggaagcgccg gtgatacgcg ccacccaatc acccccaccg
     4441 gcttgccaga agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc
     4501 ggctttggga aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa
     4561 ctgctgtgta ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt
     4621 gacccatagc aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacgggtttc
     4681 acaaaccaaa gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat
     4741 tggtgagcag gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac
     4801 taattcgccc tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca
     4861 atctatggac accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tatgcggcgt
     4921 cattgacctc cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg
     4981 tcgatcgtta tgccgatcat attttttacg gcagcggtgc cacggggatg gcactggttg
     5041 ttatcgacgg taaccagcgc gtctttcgca gttatggcga aacgcgacct ggtaataacg
     5101 ttcgcccgca gctggattcc gtcatacgta ttgcttccct caccaagtta atgaccagtg
     5161 agatgctggt gaaattgctc gaccagggga ccgtgaagtt aaacgatccg ctaagtaaat
     5221 atgctccgcc aggcgcacgc gtaccgacct acaatgggac gcccattacg ctggtgaatc
     5281 tggcaaccca taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg
     5341 tatttgtctg gccaacgcgc gagcaacgct ggaaatacct ttccactgcc aaactaaaag
     5401 ccgcgccggg cagccaggcc gcgtactcca acctcgcatt cgatctgctg gcagatgcac
     5461 tggcgaatgc gtccggcaag ccttataccc agttgtttga agagcagatt acccgtccgt
     5521 tggggatgaa agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg
     5581 ctgagcgcgg tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct
     5641 attccacgcc aggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc
     5701 agcgcagtaa ccaggctgat cgcatgcaga cgttgattta tcagcgcgcg cagtttacaa
     5761 aagtgattgg catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata
     5821 tggccccgaa agagggtcgt ccggggatta ttcagaagac aggtggtggt ggcggtttca
     5881 ttacctatat ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt
     5941 cgccgttgac gcgctttaaa aatatgagcg atggcatcaa tgacctggtg accgagctaa
     6001 gcgggaataa accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg
     6061 atagcgacca gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga
     6121 acttccgcaa cgacagaacg cgatatacgt gtacgttgat ggatataatt catcacgcca
     6181 atacgtgatc gcaattcttc attccagtca atcattgata ataacgtcgc gcggatttgt
     6241 tcgtaggaat tatgcccaat gagctgaacg tcgcgtaatt ccagaattct attttgccag
     6301 gctaaccagt aaaaagcgtc tcgccagagt tgattttgtt caataagagt aatggtttgt
     6361 ttggctggca aatgatatcc cgtacagttt ccttctgata ttaatttgta tggtatatcg
     6421 tttttcatta aaccatcagc cagccccata atataaggag cctgtgtaat accgataagt
     6481 acgttttctt ctctacgcaa agagataacg ccctctaaaa taacgaaagt atcttcgcta
     6541 ttgacatcac tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgttt
     6601 ctggacaaac atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt
     6661 tgcgcgtcct ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta
     6721 cctatttctt cggagtgaag aatattacca ggtatattta acacccacat tcgcggacca
     6781 gtcttgatct acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt
     6841 ggtgaagctg aactgagtac ccagaccaac acgtaccgca gaccctccag taccgttatc
     6901 gatggaatca ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt
     6961 gaagtacgga gtcagagcct ggtcttcgct gtaggtgaag gtataacctg catctacacc
     7021 cagttcataa cgcatgctgt cgtaagactg accatcaact ttcatgtcgt tgctcagctg
     7081 gtaatcatca ccagactgga acagaccaga aacgctgccg taaggagtca cgtaacctgc
     7141 atcacccagt ttgaagtcgt aaccggcttt caaaccaaag ccccaagcgt cggagttggt
     7201 gctgccgtca acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga
     7261 gtagctcaag ctaccgtcaa caaagacgtt gttcgcgaag tgagcagaag agtagatgta
     7321 ggcagtctgg ctgtcttgat ccacctgacc agaacggtca ttcatatcac ctttagcgaa
     7381 gcctgcagcc gcaccgacga tccacttagc gttgttaccg tcaattttgg tatcaacacc
     7441 gaccatgatg ccgttaacat cctgatcata gttgatggtg ccgttgtcgc cgttgaagtt
     7501 accaccgaag tagcttaccc atgcgccgcc gttatcagcc agaccatgac gagagttggt
     7561 cagacgagta ccaacggtgt cttgttccag gttccagata ttggtgttcg cagacgggat
     7621 gctcagcgcc atgttagcgt agtcggtcag ctccatctgt tgcagaacaa cggtgttacc
     7681 acgctgctca gcctgatagg tgtatgcacc caggtcagct ttgttagcag cagagaaggt
     7741 cgcgttgctg tttttgtcgt tgacgtagat cagctcgttg ccgttgtagt cagcgataga
     7801 accagcacca gtagcgttgt cgatacgaac tttgtagtta ccggttgccg caacaacgtt
     7861 gtctacagag ctgttatcca gttcagtacc gttgtctacg tcgccgttac cgttgatagt
     7921 cagatgacca tcagagttca tagcaacaac accgtagccg tagttagcct gagcggtatc
     7981 ccaagtacgg tcgttcacca ggtcagcgtt cagaacataa tcgctgctgt tgatatcgaa
     8041 cacaccagaa actacattgc cgttgtggtc agcgatgttg ccgctagtca gttccagttt
     8101 gtcagtacgg aaagcttcag tatcaaactg atcaacgttc acatccagta caccaccgtt
     8161 agttacggtg atggtattgg catacaggtg agaatagtta ttccagccag tagattcggt
     8221 taaattaacg gtactgtagc tatcaacagt caggtggtct gtagcaacca aaccatcttc
     8281 accaatattc agtgaggagc cgttggttaa accaatggta tctgagacca gagaggaatc
     8341 agtaacattt acctgagagc cattatttac agctaatgta tcaattactg aactattaac
     8401 ggtattccat tctgaaccat tgttcagtgc tacgttaaat acgccgctct ggaatacctt
     8461 gttgtcaata atacgacccc aatcgttagc ttcgatagtg gcaccagggg tcaggctgtt
     8521 cggcgcaaca tcatacatat ctgccggagc aattacttca gcactcatgt ttgctgagcc
     8581 aacccactta ctaccgttgt tcagagtaat gttcagttca tcaacgttct gagcatcatc
     8641 tacccaacca ccgttggtat caaggacacc gtcaccattg ctgtcatggc cggttgaata
     8701 gaagaatacg ccatcgttat tccagtggct tgtgaactcg acatcaccca acagggttga
     8761 atggtcaaag tttacattgt ttttcatgga ataatttgag ccagcattgt cagagaatga
     8821 cagagctacg tcattttcgc caccattacc agcgtaatca cttggttcag cagatttacc
     8881 gaagaagcca ttgctttctt ccagcggagt ctgtgaccct gaagttacag tggagttaga
     8941 cacattaatg ttgttgtccc actgatgata ttcgctagta taagtcgtgt tgcttgtatt
     9001 gccttcagac agcgcaatac aagctacacg ggagttgtta gtaatattga tattgctttc
     9061 aacatcaaga tttactgcaa cacccagagc ggtatagaac gtttcattgg ttgacttaga
     9121 tttatcacca tcaagatatg tgtcagtgaa gtaaagagct tcatagtcat catcaatggt
     9181 ggaattggat acgttcagag tgaatacgtc accatcgtac caattgtagt caaggtcagt
     9241 agtgattccg ttatagaaat tatagccatt ataggtctgt ggagttacaa cttcaccttt
     9301 agcattaact tctgcaggag taatgttgta ataaccaaat ggcagttcag aggtgattgc
     9361 cccatgaatt actgaattgc tgatatctac ctggttaaca gaaccgctag cgtaatcatg
     9421 attcaagtaa taagtagaaa tggtaccgtt aacggtatcg cctttaaaga cggtatcgaa
     9481 atcgccattg gcattgttat agtaaacaaa accattgtaa gttacgtagt taccgtctga
     9541 acccatcggg tccatgccgc cagcatcggc aattccatca ttgtcagtgt catacggcca
     9601 aactaaatcg gtcgtggtgt tgtcatgata cccccaggta tcatagtttg ctgcattagc
     9661 ctgagaggtg atagccagag tgcaagccaa tgctaattgt gatactacaa gtttcttttt
     9721 ccaggagtgc atttgtcatc cctcctcagg gacgtaataa gttgatccat caattatcaa
     9781 tgcatagaaa acatattatg tttcgcgttt acgattatgc ggcgttatag aggaatggtt
     9841 caattattct ttcccctaag tccgattgcg gacatatata aagcacgtga acacacacaa
     9901 agaaagaaat aacaaaaaca caatatttca atacgttaat gaaagcgtt
//
LOCUS       DADTFG010000165         9918 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.166, whole genome shotgun sequence.
ACCESSION   DADTFG010000165 DADTFG010000000
VERSION     DADTFG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.166"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(236..2809)
                     /gene="clpB"
                     /locus_tag="J1138_003983"
     CDS             complement(236..2809)
                     /gene="clpB"
                     /locus_tag="J1138_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBA8455717.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2939..3670)
                     /gene="pgeF"
                     /locus_tag="J1138_003984"
     CDS             complement(2939..3670)
                     /gene="pgeF"
                     /locus_tag="J1138_003984"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBA8455718.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRTGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGGRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3667..4647)
                     /gene="rluD"
                     /locus_tag="J1138_003985"
     CDS             complement(3667..4647)
                     /gene="rluD"
                     /locus_tag="J1138_003985"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBA8455719.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            4782..5519
                     /gene="bamD"
                     /locus_tag="J1138_003986"
     CDS             4782..5519
                     /gene="bamD"
                     /locus_tag="J1138_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBA8455720.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5789..6130
                     /gene="raiA"
                     /locus_tag="J1138_003987"
     CDS             5789..6130
                     /gene="raiA"
                     /locus_tag="J1138_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBA8455721.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            6234..6281
                     /locus_tag="J1138_003988"
     CDS             6234..6281
                     /locus_tag="J1138_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455722.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6379..7539
                     /gene="pheA"
                     /locus_tag="J1138_003989"
     CDS             6379..7539
                     /gene="pheA"
                     /locus_tag="J1138_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBA8455723.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSAINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAVMQKALKDLGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7582..8703)
                     /gene="tyrA"
                     /locus_tag="J1138_003990"
     CDS             complement(7582..8703)
                     /gene="tyrA"
                     /locus_tag="J1138_003990"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBA8455724.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8714..9784)
                     /gene="aroF"
                     /locus_tag="J1138_003991"
     CDS             complement(8714..9784)
                     /gene="aroF"
                     /locus_tag="J1138_003991"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBA8455725.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2518 a         2636 c         2322 g         2442 t
ORIGIN      
        1 caagcattta tttttgcttt ctctgccgga attctcagga gaaccccgct gacccggcgg
       61 cgtgtttgcc gttgttccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac
      121 aagcaaaaaa tataaaaact ttattgttcg ctcattttcc aggcaaaaca tctttaatat
      181 agtcttttcc gtctaactta tagacaaaaa cgagccccga aggctcgttt tatcattact
      241 ggacggcgac gatccggtct tcattaactt ccaggcgaat aactttaccc ggaaccaatt
      301 caccagacag tatttgctgt gccagcgggt tttcgatctg ctgctggata gcacgcttca
      361 gaggacgtgc accatagacc ggatcgtaac cgttctcgct cagcagtttc agcgcctcgt
      421 cagaaatgtg gatttcataa ccacgttctt ccagacgttt gtacagacgt ttcaactgaa
      481 tctgcgcaat cgacgcaatg tgttgttcac ccagcggatg gaagacgacg acttcatcga
      541 tacggttaat gaattccgga cggaagttat ggcttaccac accgagcacc agctctttca
      601 tgtgcgcata atccagttca ccaaaacgtt cctgaatcag atcggaaccg aggttagagg
      661 tcataatgac gaccgtatta cggaagtcga ccgttctccc ctgcccgtca gtcagacgcc
      721 catcatccag tacctgcaac agaatgttga agacatccgg atgcgctttt tccacttcat
      781 ccagcaggat gacggaatac ggacgacgac gcaccgcttc ggtcagatag ccaccttctt
      841 cataaccaac atatcccgga ggcgcaccaa ccagacgaga caccgagtgt ttctccataa
      901 actcggacat atcgatacgg accatcgcct cgtcgctatc aaacataaag ttcgccagcg
      961 ccttacaaag ctctgttttc cccacacccg ttgggccgag gaacaggaat gaaccaatcg
     1021 ggcgatttgg atccgccagc cccgcacggc tacgacgaat agcgttagat accgcatcaa
     1081 ccgcttcgtt ctgaccaata acgcgatggt gcagttcttg ctccatacgc agcagttttt
     1141 cgcgctcgct ttccatcatg cgagaaaccg gaattcccgt ccaacgcgcc agcacttcag
     1201 caatttcggc atcggtcact ttattacgca acagacgcat agttttgcct tcgagctgcg
     1261 ttgcggcttc cagttgcttt tccagttccg ggattttgcc gtattgcagt tcagacatcc
     1321 gcgccaggtc acccacacgg cgagcctgtt caatagcgat tttcgcctgt tccagttccg
     1381 ctttaatggt ctgcgtacca gaaagcgagg ctttctctgc tttccactct tcttctaact
     1441 cggagtactg acgttctttg tcgctcagtt cttcgttgag catatccagg cgttttttac
     1501 tggcttcatc agactctttc attaacgcct gttgttccag tttgagctgg atgatacgac
     1561 gatcgagtcg gtcgagttct tctggttttg agtcaatctg catacgaatg ctggatgctg
     1621 cttcatcgat caggtcgatg gctttatccg gcagctgacg gtcagcaatg tagcgatgag
     1681 acaacgtcgc tgctgcaaca attgccgggt cagtaatttg cacatggtgg tgcagctcgt
     1741 aacgttcttt caggccacgc agaatagcaa tggtatcttc aacagaaggc tcggcaacaa
     1801 acactttctg gaaacgacgt tccagcgcag catctttttc gatgtactgg cgatattcgt
     1861 caagcgtcgt ggcacctacg cagtgcaatt caccacgcgc cagtgcaggt ttcagcatgt
     1921 ttccggcgtc cattgcgcca tcggctttac ccgcgccgac catggtatgt aattcgtcga
     1981 taaacaggat gacgttgcct tcctgtttag caagatcgtt aagtacgcct tttaaacgtt
     2041 cttcaaactc accgcgatat ttcgccccag ccaccagcgc gcccatatcc agcgccagta
     2101 cgcggcggcc tttcaaccct tcgggcactt cgccgttgat aatacgctgc gccagacctt
     2161 caacgatggc agttttaccg acgccgggtt caccaatcaa taccgggtta tttttagtac
     2221 gacgttgcag cacctgaatg gtacggcgaa tttcttcatc acgaccaatc accggatcga
     2281 gtttgccttg ttcggctcgt tcggtaaggt cgatggtata ttttttcaaa gcctgacgtt
     2341 ggtcttcagc accttgatcg ttcacgcttt cacctccacg catttgttca atcgcttgag
     2401 taatgttggc ggtggtcgcc cctgctgctt tcaggatgtc ggccagcgtg ccgcgagact
     2461 caagtgccgc cagaacgaac agttctgacg agataaagtt atcaccacgt ttttgcgcca
     2521 gcttgtcgca aagattaaga acgcgcacca gatcctgtga tggctggaca tcaccaccag
     2581 taccttcaac ctgcggtaaa cgatttaatg cctgattgat atctgtgcgc aactggccag
     2641 catttatgcc agcggatgtt aataaaggac taaccgaacc cccttcctga ttcagcaggg
     2701 cgctcattaa atgaagtggt tcgatgaatt ggttgtcgtg cccgagtgca agtgattggg
     2761 catcggcaag agcaagctgg aatttattag taagacgatc cagacgcata actcctccca
     2821 taacggatca aagttgctac tggagattaa atgaggtcat ccctcaatta ttcaaggtta
     2881 ttgaccagat taatgtgaaa agaaaatcac gcgtaccgga tcgtcttgat tctttaggtt
     2941 atatcagcca aatgaaactt gccatacgac cggtggtctt gtcgcgacga taagagaaga
     3001 aagtctcatt ttccgtatat gtacaacggc cgccgccgaa aatttgttca acgccaacgc
     3061 tcgccagacg ctgtcgggca aggagataaa tatccgccag atacttatca ccatgctgaa
     3121 tgaaagctgt actcgctttg gcgtctactg ccataaacgc ctcgcgaacc tccgccccca
     3181 cttcaaacgc gcgtggacca attgccggcc ctaaccaggc gagaatattt tccggattat
     3241 cagcaaaaca agaaaccgtc tcttccagca cgccagaaca cagcccgcgc cagcctgcat
     3301 gagcggcggc aacttccgtt cccgtacgat tgcaaaacag cacagggagg cagtcagccg
     3361 tcatcactgc gcaaaccgtg ccgggcgtat tgctataaga ggcatcagct cgttttgagg
     3421 cataaggttc gccagtaagc ttaagcacat ctttgccgtg tacctgctca agccagaccg
     3481 gttttgaagg caaattgccc gcagcaaaaa gtcgcttgcg attctcttca acgtgatccg
     3541 ggttatcacc acaatgagcg ccaaggttga gagagtcgta aggcggcaag ctcacgccgc
     3601 cgatacgggt agagctacag gccgcaacac cttttggcag cggccactgc gggacaatca
     3661 gcttactcat aaccagtcca cttcatcctt atgctcttcg aaatcggcgc gcatctcctc
     3721 aatcagctcc accatatctt gtggaatagg cgcatgccat tccatttcga taccggagat
     3781 cgggtgataa agacgcagca tggttgcatg aagcgcctgg cggtcaaact tacgcagcgt
     3841 ggagataaac gcttccgaag caccttttgg cggacgcgga cgaccaccat aaaccggatc
     3901 gcccaccagc ggatgagtga tatgggccat atgcacgcgg atctggtgcg tacgtccagt
     3961 ttccagacgc aaccgcagac gcgtgtgcac acggaagtgt tccatgatgc gatagtgagt
     4021 gaccgccggt ttacccatcg gatgcaccgc catgtgagta cgtttggtcg ggtggcggct
     4081 gattggttca tctactgtac cgccagcggt catatgacca attgccaccg cttcatactc
     4141 acgagtaatt tcacgccgtt gcaaagattc gactaaacgc gtctgagccg gaacggtttt
     4201 tgccacaacc atcaggccgg tggtgtcttt atccagacga tggacgatgc ccgcacgcgg
     4261 tacatcggca atgggcggat agtaatgaag caacgcattc agtaccgtac catccgggtt
     4321 acccgcgcca ggatgtacca ccaggtcgcg tggtttatta atgacgatga tatcttcatc
     4381 ttcatagacg atatccagcg ggatatcctg cggttcaaaa cgcgcttctt cttcaatctc
     4441 agcgttgatg gccacctgct cgccacccaa tactttttct ttcggcttat cacacacttt
     4501 gccgttaacc agcacgcgct ggtcgaggat ccattctttt attcgcgaac gtgaataatc
     4561 cgggaacatt tcggccaaag cctgatctaa gcgttgaccg agttggtttt cggacaccgt
     4621 tgcagtgagc tgtactcgtt gtgccatata tactgcttct tcgtttatcg ttggggtttt
     4681 acggctttgc cgtttaatat agtgtgctat tgtagctggt cttaaccggg agcaggaaca
     4741 gagaatctcc cgtattacat tttgaggaaa gtcaaaacgt catgacgcgc atgaaatatc
     4801 tggtggcagc cgccacacta agcctgtttt tggcgggttg ctcggggtca aaggaagaag
     4861 tacctgataa tccgccaaat gaaatttacg cgactgcaca acaaaagctg caggacggta
     4921 actggagaca ggcaataacg caactggaag cgttagataa tcgctatccg tttggtccgt
     4981 attcgcagca ggtgcagctg gatctcatct acgcctacta taaaaacgcc gatttgccgt
     5041 tagcacaggc tgccatcgat cgttttattc gccttaaccc gacccatccg aatatcgatt
     5101 atgtcatgta catgcgtggc ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt
     5161 tctttggcgt tgaccgtagc gatcgcgatc ctcaacatgc acgagctgcg tttagtgact
     5221 tttccaaact ggtgcgcggc tatccgaaca gtcagtacac caccgatgcc accaaacgtc
     5281 tggtattcct gaaagatcgt ctggcgaaat atgaatactc cgtggccgag tattatacag
     5341 aacgtggtgc atgggttgct gtcgttaacc gcgtagaagg catgttgcgc gactatccgg
     5401 atacccaggc tacgcgtgat gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga
     5461 tgaatgcgca agctgaaaaa gtagcgaaaa tcatcgccgc aaacagcagc aatacataac
     5521 agaaacctga aacacaaaac ggcagccttg agctgccgtt tttttattct gttagttgtg
     5581 aaactgaagc gatttagtca ctatcgatct catcaaatat ggctcgcttt gagatattcc
     5641 tcaagtaaaa aaacacctct tcctgcgatt tctcacaaaa aagattcgtt gacaaaaagt
     5701 gacaaaatta tgagatttcc atcacacatt ttgacatcag gaacgttatg ctgaattcac
     5761 caagacggga agacaagagg taaaatttat gacaatgaac attaccagca aacaaatgga
     5821 aattactccg gccatccgcc aacatgtcgc agaccgtctc gccaaactgg aaaaatggca
     5881 aacacatctg attaatccac atatcattct gtccaaagag ccacaagggt ttgttgctga
     5941 cgccacaatc aatacaccta acggcgttct ggttgccagt ggtaaacatg aagatatgta
     6001 caccgcaatt aacgaattga tcaacaagct ggaacggcag ctcaataaac tgcagcacaa
     6061 aggcgaagca cgtcgtgccg caacatcggt gaaagacgcc aacttcgtcg aagaagttga
     6121 agaagagtag tcctttatat tgagtgtatc gccaacgcgc cttcgggcgc gttttttgtt
     6181 gacagcgtga aaacagtacg ggtactgtac taaagtcact taaggaaaca aacatgaaac
     6241 acacaccgtt tttcttcgca ttctttttta ccttcccctg aatgggaggc gtttcgtcgt
     6301 gtgaaacaga atgcgaagac gaacaataag gcctcccaaa tcgggggcct tttttattga
     6361 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6421 agcgcgctgg atgaaaaatt attagcatta ctggcagagc gacgcgaact ggccgtcgag
     6481 gtgggaaaag ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg tgaacgcgat
     6541 ttgctggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6601 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     6661 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6721 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6781 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6841 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6901 cataccagtt tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6961 tccggcacta ctgatttatc cgccatcaat acggtctaca gccatccgca gccattccag
     7021 caatgcagca aattccttaa tcgttatccg cactggaaga ttgaatatac cgaaagtacg
     7081 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     7141 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcga
     7201 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtttc tgaccaggtt
     7261 ccggcgaaaa cgacgttgtt aatggcgacc ggacaacaag ctggtgcact ggttgaagcg
     7321 ttgctggtac tgcgcaacca cagtctgatt atgacccgtc tggaatcacg tccgattcac
     7381 ggtaatccgt gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggta
     7441 atgcaaaaag cattgaaaga tttaggggaa atcacccgtt cgatgaaggt attgggctgt
     7501 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaagtgc cggatgatgt
     7561 gaatcatccg gcactggatt attactggcg attgtcattc gcctgacgca ataacacgcg
     7621 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7681 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7741 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag
     7801 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7861 agagagcgcc agtagttgct caagctgaac attttcttct gccaggtgca gcccataagc
     7921 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7981 gctaatacga tgcaaccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     8041 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     8101 gccgaacatc gggtgtaacc ccagcaccgg gccatcgtgc gccgccagca tggcctgtaa
     8161 tggtccattt ttcaccgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8221 tttgccgata acttgctcag taacgtggat gggcacacta acaatcacca ttccggcatc
     8281 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8341 cgagagtgtc agcatcttct cgaacagacg tcccatctga ccgccaccac cgacgataac
     8401 cactgggcgc agtgacggac aaagcgtttt aaatcctttg tcgttttcac tggagtaaga
     8461 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8521 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8581 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     8641 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8701 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     8761 cacgcagcaa ggcatctgtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8821 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
     8881 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8941 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     9001 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     9061 agtttggcgc tttaccaccg cgcaggatca catgcccgtc cggattcccc tgagtttgta
     9121 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcac
     9181 gcatagcgtt aattgccgtt gccagactgc catcggtgcc gtttttaaaa ccaaccggca
     9241 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9301 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9361 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9421 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9481 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9541 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9601 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttt
     9661 tacgcgagtc agcaatctgg gcttcttgtt gcaggcttaa tggaaaagcg gccttcagtt
     9721 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtctttct
     9781 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9841 aacgataaca cataaagtaa agttttcaat ccatatttcg tacatatttc tttacataag
     9901 caatttattc gcgctttt
//
LOCUS       DADTFG010000166         9879 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.167, whole genome shotgun sequence.
ACCESSION   DADTFG010000166 DADTFG010000000
VERSION     DADTFG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.167"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            252..875
                     /gene="rcsA"
                     /locus_tag="J1138_003992"
     CDS             252..875
                     /gene="rcsA"
                     /locus_tag="J1138_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBA8455726.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTMTSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(919..1161)
                     /gene="dsrB"
                     /locus_tag="J1138_003993"
     CDS             complement(919..1161)
                     /gene="dsrB"
                     /locus_tag="J1138_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBA8455727.1"
                     /translation="MPGKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            1270..1497
                     /gene="yodD"
                     /locus_tag="J1138_003994"
     CDS             1270..1497
                     /gene="yodD"
                     /locus_tag="J1138_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBA8455728.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1795..2610
                     /locus_tag="J1138_003995"
     CDS             1795..2610
                     /locus_tag="J1138_003995"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBA8455729.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQNIDGFPRIISGISHSEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(2607..4301)
                     /gene="yedQ"
                     /locus_tag="J1138_003996"
     CDS             complement(2607..4301)
                     /gene="yedQ"
                     /locus_tag="J1138_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBA8455730.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTSMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKACPLAKLCHTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4472..4654)
                     /locus_tag="J1138_003997"
     CDS             complement(4472..4654)
                     /locus_tag="J1138_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBA8455731.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(4733..5650)
                     /locus_tag="J1138_003998"
     CDS             complement(4733..5650)
                     /locus_tag="J1138_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBA8455732.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5823..6743
                     /gene="yedA"
                     /locus_tag="J1138_003999"
     CDS             5823..6743
                     /gene="yedA"
                     /locus_tag="J1138_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBA8455733.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(6732..7202)
                     /locus_tag="J1138_004000"
     CDS             complement(6732..7202)
                     /locus_tag="J1138_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HBA8455734.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7183..8601)
                     /locus_tag="J1138_004001"
     CDS             complement(7183..8601)
                     /locus_tag="J1138_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBA8455735.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8668..9363)
                     /locus_tag="J1138_004002"
     CDS             complement(8668..9363)
                     /locus_tag="J1138_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBA8455736.1"
                     /translation="MELQHWQAQFDNWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(9403..9768)
                     /locus_tag="J1138_004003"
     CDS             complement(9403..9768)
                     /locus_tag="J1138_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA8455737.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGGIAWYTRPRPARCRRYE"
BASE COUNT         2446 a         2606 c         2376 g         2451 t
ORIGIN      
        1 aaaaataatg tctgcgaaca tcttccagga tactcctgta gcgaaatatt tgttttaagc
       61 tcactcacat atcgcaacat ttactttact ttaagacaat tccaggcaaa ttatacaaca
      121 ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg
      181 ttgttccgtt tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt
      241 gagggtatgc catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa
      301 ccgggtatct gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg
      361 atgaccttgc catagcttgt gattcacagc gcccttcagt ggtgtttatt aatgaggact
      421 gtttcatcca cgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca
      481 atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg
      541 tcagaaaaaa tttattgatc agttctaaat cgattaaacc ggaatctctc gacgatatcc
      601 ttggcgatat tctgaaaaaa gagacaacga tgacctcgtt tttaaatatg ccgacgttat
      661 cattgagccg aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc
      721 aaatctccga ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta
      781 aacgtaagat caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata
      841 atgtgactaa tggtattttt gtcaacatgc gctaacacat tctgactggt ggtttcccac
      901 cagtcaggct gaataagatt actctgcttt ctcaacaaag ataccgtcct gatgccctgc
      961 ttcattaaag aaccagatgc cgagcgggta gtcttccagc gaaaccaggt acattgtgcc
     1021 ttcactaaac tcctcaactg ccagtaccac gccaggacga cgcggaccgc catccgtttt
     1081 gactgttacc cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga
     1141 acaatttcgt tttcctggca gcgccaggcg cgcgcgagtg ctgattttct cgacggtcta
     1201 tacttaagag atgccaggcg gacttaacaa ctggcggcaa caacagagta acggttgcga
     1261 ggaaagatga tgaaaaccgc aaaagagtac agcgataccg caaaacgtga ggtcagcgtc
     1321 gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc
     1381 cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg
     1441 gcggatgcct tcgaactgga tatccatgac ttcagcgtct ctgaagtgaa tcgttgaatg
     1501 cacaataaaa aaatcccgac cctgaggggg tcgggatgaa acttgcttaa gcaagaagca
     1561 cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatct gcaatttttt
     1621 tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg
     1681 acgcgcgtca ccccttggtg gctctggtgt caacgggatc gctcccgaaa aaatcatctt
     1741 tgattatttg tgctgttttg agaatcatcc tggtaagggg agtactgcgc gaccatgttt
     1801 tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct ggacagtcat
     1861 agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc caatattccc
     1921 gtcattctct gtagcagtaa aacttcagcg gaaatgctgt acttgcaaaa aatgttgggg
     1981 ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg
     2041 cagaatatag acggttttcc acgcatcatc tcaggtatta gccatagcga aatctgccag
     2101 gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac
     2161 gatgcaacca ttgccgaatg gacgggatta agccgtagcc aggcggcgct gacgcagctg
     2221 catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc
     2281 gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg
     2341 gatgcctctg ccggaaaaga tcaggctgcc aactggatta tcgcgaccta tcaacaattg
     2401 tcaggcaaac gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg
     2461 gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt ccatcttcat
     2521 gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa
     2581 gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc
     2641 ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca
     2701 taatcaccgg tttcctcgct gctgcttacc cccagcgagg cactgatgcg tatcgttgta
     2761 ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc
     2821 gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc
     2881 ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca
     2941 cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc
     3001 tgaatgacag aaaaaggatg ttggtgcgtg tgacacaatt tagcgagcgg acaggctttt
     3061 tcgaacaatg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc
     3121 aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata ccaggagagg
     3181 agtaacatgc tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa
     3241 tcgccgcgca cgccttcgct tagcgtatgg acgcgcacca gcacaccgtc gaaatggtcc
     3301 agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc
     3361 attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca
     3421 ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg
     3481 tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc
     3541 agcacgccat accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca
     3601 gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct
     3661 cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca
     3721 ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc
     3781 gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt
     3841 gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggagatt cccctcgctg
     3901 actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc
     3961 caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa
     4021 tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg
     4081 ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctggctg
     4141 gatatatagg catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc
     4201 aataccacga taaagcagag attaacgaca tgaccaggcc ccaggcggcg tgcgagtttt
     4261 ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg
     4321 cttttttatg attctggcat aacccggcga tagcgtaccg caaaacagcg ataattttat
     4381 aatggtggca tgataagacg cgactatcat tcattacagg cattgggaca ccaggccgga
     4441 tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct
     4501 cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc
     4561 aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt
     4621 taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac
     4681 ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag tattaatgtg
     4741 cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg ccgatgataa
     4801 atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc
     4861 cggcgaaatg ttcaatggcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc
     4921 cgacgaggaa catcgccagc gtgccgacaa tcgataatgc tttcatcagc cagggcgcga
     4981 taatcaataa ccctttacct aatgcctgca tcagcgcgct ggatttttcc gccagccaat
     5041 accccaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta
     5101 ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcttct gccacaatcc
     5161 ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc gcccctttaa
     5221 ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctggc
     5281 tctgcgccgg atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc
     5341 cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa
     5401 acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg
     5461 ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat
     5521 tgagcgataa atcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca
     5581 cggagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg
     5641 ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca
     5701 gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct
     5761 cgccagcaga atgattttca cgtttactat gaaccacctt tttatttccg ccgtgaggga
     5821 ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt
     5881 ggggctcaac ctattttgtc attcggattg gcgtggaaag ctggcctccg ttaatgatgg
     5941 cgggcgttcg attcctggca gccggtattt tattgatggc atttttgcta ctgcgcggac
     6001 acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg
     6061 ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg
     6121 ccgtagtggt tgcaaccgtg cccctcttta ccctgtgctt cagccgcctg tttggcatta
     6181 aaacgcgcaa actggaatgg gtgggtatcg ccattgggct tgccggaatc atcatgctca
     6241 atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga
     6301 ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta gggatgatgg
     6361 cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg
     6421 gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc tatctggcgc
     6481 tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat gtcagtccgg
     6541 ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac
     6601 tgggtggcga aacgctgtcg aaaattgaat ggctggcgct cggcgtaatt gttttcgcgg
     6661 tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta
     6721 ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
     6781 tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc
     6841 ccacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg caagcgactg
     6901 atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa ctcggttcga
     6961 gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat
     7021 atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga
     7081 acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta
     7141 tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc
     7201 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
     7261 tgccaccgcg gcaaagaccg gcacgaccac cgagttaccg aactggcgat aggcctgagt
     7321 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
     7381 gcattcacga ggtgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc
     7441 tttctcaccc gtggccatat cccagccgcg gtcgattaaa atttccgcgc catctttgta
     7501 ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata
     7561 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
     7621 caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac
     7681 tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag
     7741 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc
     7801 gtcgatgatt ttcggatcgt ccggcccgtt atctgctgcg tcggcaacgt catagcccag
     7861 ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt
     7921 tttgacattt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc
     7981 aaagaacagc gtaccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
     8041 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccagccagta aaacatcgtg
     8101 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg
     8161 actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg
     8221 gttggcttta taagtgcgta cagcatgttt gttccattcg ctggtaaaca cgcactgtcc
     8281 gccaatcgat tcaaaaccgc gacggatgcc gccaattccg gcaaatagat cgataaagcg
     8341 aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa
     8401 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
     8461 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcgttt taacatcata
     8521 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
     8581 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
     8641 taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt
     8701 gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca taaactcgac
     8761 taaaaattgc gcgttgtgct gtgccagctg cttgcctctt gcggtttgca tggtttgcgg
     8821 taatttcagt aactttgtct ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg
     8881 atgctgtgcg aacggatctt cgccatcaaa cagcgccaag cccaatgccc ctgaaacggc
     8941 aaatacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat
     9001 tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg
     9061 acaaacggcc tcaattttct ccgccggaaa ttgcgcgaac tcttcacgga gcaggcgacg
     9121 cgtctcttct gccgccagga ttgaagaacg ctgccgttgc ggatgatttt tcgccaggct
     9181 cacgatatcg tgaaaataac aggcggttaa aatcaccagc atatcgacat cgtcatctgc
     9241 cgccagtttc tgtgccgtag cccagacacg gcgaaaatga cacaagtcat gcgccgcgtc
     9301 ctgatgttga tgatggtttt tcaaccagtt atcgaactgt gcctgccagt gttgtaactc
     9361 catacgcgct ccgttgccaa agagtcgcaa ccttagcagt ttttattcat agcgtctgca
     9421 acgtgcggga cgaggtcggg tgtaccatgc aatgccgcct aacaaggcac caaccagggc
     9481 cataaacaga aatccgacca atgctcctaa ccacttcccc gcggtagatc ctaaatcact
     9541 ttcaaatccc gacacggggg catcggggat ccggctcatc acccagatgt atcgcgccat
     9601 tgcccagacg aaatagccac accatgtata gaaagcccac aaagccaatt tgccgccagg
     9661 gctgcgagag agtctctctt ccatctttgt cgaaccgtat tccattttga gataacccgg
     9721 aaaattcaga cttagaacta ttatgtgata aaagtcacat ttttccacat tggatgagcc
     9781 agtcaagact caatcatttt ttgaacaaat agataaattt tgcgcaacgg cggtgaatac
     9841 aggaaaaatc gatttgaacg atgttattta tctttgatt
//
LOCUS       DADTFG010000167         9814 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.168, whole genome shotgun sequence.
ACCESSION   DADTFG010000167 DADTFG010000000
VERSION     DADTFG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.168"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..389)
                     /locus_tag="J1138_004004"
     CDS             complement(<1..389)
                     /locus_tag="J1138_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBA8455738.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHL"
     gene            complement(379..615)
                     /locus_tag="J1138_004005"
     CDS             complement(379..615)
                     /locus_tag="J1138_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBA8455739.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            complement(755..994)
                     /locus_tag="J1138_004006"
     CDS             complement(755..994)
                     /locus_tag="J1138_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBA8455740.1"
                     /translation="MFRVIDPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRRK"
     gene            complement(978..1304)
                     /locus_tag="J1138_004007"
     CDS             complement(978..1304)
                     /locus_tag="J1138_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBA8455741.1"
                     /translation="MANLQLAVKSEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTITHPHFGDKPVKVFAIKVNISTEYQSAQHKV
                     KNVQSD"
     gene            complement(1304..1525)
                     /locus_tag="J1138_004008"
     CDS             complement(1304..1525)
                     /locus_tag="J1138_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBA8455742.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYLAVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELIRKQRGIK"
     gene            complement(1624..1905)
                     /locus_tag="J1138_004009"
     CDS             complement(1624..1905)
                     /locus_tag="J1138_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBA8455743.1"
                     /translation="MHFSGSGLHILCAYACRHGACSVTPQQENALRSIARQANSEIKK
                     VRQQFPDKNVNDICRSVLKKHRETVTLKGFTPTHLSLAIGMLNGVFKER"
     gene            complement(1916..2107)
                     /locus_tag="J1138_004010"
     CDS             complement(1916..2107)
                     /locus_tag="J1138_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HBA8455744.1"
                     /translation="MHKASPVELRTSIDLAHSLAQIGVRFVPIPAETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(2080..2262)
                     /locus_tag="J1138_004011"
     CDS             complement(2080..2262)
                     /locus_tag="J1138_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HBA8455745.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFHGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            complement(2259..2939)
                     /locus_tag="J1138_004012"
     CDS             complement(2259..2939)
                     /locus_tag="J1138_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBA8455746.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKNWPDMKMSYFHTLLAEVCTGVAPEVNAKALEWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESIRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(2936..3721)
                     /gene="bet"
                     /locus_tag="J1138_004013"
     CDS             complement(2936..3721)
                     /gene="bet"
                     /locus_tag="J1138_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HBA8455747.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRTSSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(3727..4023)
                     /locus_tag="J1138_004014"
     CDS             complement(3727..4023)
                     /locus_tag="J1138_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBA8455748.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLSQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(4099..4305)
                     /locus_tag="J1138_004015"
     CDS             complement(4099..4305)
                     /locus_tag="J1138_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HBA8455749.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            complement(4783..5646)
                     /locus_tag="J1138_004016"
     CDS             complement(4783..5646)
                     /locus_tag="J1138_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455750.1"
                     /translation="MDCDALQDIKISLRYDGKDALNHEIDLNCLGESLKGFSKVLSTA
                     ASFSVTQKYSKYINYQEVKVYAREAKANCFTLEAVLNFATQNQLFSGIAATILGAILQ
                     YIFARNSNKKDEMKALQQSLEKAIEALGNKDAGTIDKLISLIDRMAVELRPSVRQAVS
                     PIGNTCDQISVATNVDGCLLKVNERDKAEIDRLDDDEVLGLREYRAFLTEFDAQNMTA
                     KIILDGDDSKKRITAEISDPAAGKKNNPYIRALSAYISTKGDPSAVFTITAKATVKKG
                     QINRLFIVDAK"
     gene            complement(5713..6405)
                     /locus_tag="J1138_004017"
     CDS             complement(5713..6405)
                     /locus_tag="J1138_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8455751.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPTVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            6516..6743
                     /locus_tag="J1138_004018"
     CDS             6516..6743
                     /locus_tag="J1138_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8455752.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            6774..7313
                     /locus_tag="J1138_004019"
     CDS             6774..7313
                     /locus_tag="J1138_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBA8455753.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAEDN
                     QDVVAALIVNEYRAQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            7400..8329
                     /locus_tag="J1138_004020"
     CDS             7400..8329
                     /locus_tag="J1138_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBA8455754.1"
                     /translation="MANTAEIFNFPVPDAAQKEPRVADLDDGYTRIANELLEAVMLAG
                     LTQHQLLVFLAVMRKTYGFNKKLDWVSNEQLSELTGILPHKCSAAKSVLVKRGIFIQS
                     GRNIGINNVVSEWSTLPESGKKNKVYLKEVNLPESGKKSLPKSGKGTYPNQVNTKDKL
                     TKDNIKPFSSENSGESSDQPENDLPVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTI
                     APSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWT
                     QLEINRNKQQAGMIASKPKLDLTNTDWIYGVDL"
     gene            8326..9027
                     /locus_tag="J1138_004021"
     CDS             8326..9027
                     /locus_tag="J1138_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBA8455755.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLAKRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQIPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGARV"
     gene            9024..9311
                     /locus_tag="J1138_004022"
     CDS             9024..9311
                     /locus_tag="J1138_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HBA8455756.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAMYGRG"
     gene            9597..>9814
                     /locus_tag="J1138_004023"
     CDS             9597..>9814
                     /locus_tag="J1138_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006176174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455757.1"
                     /translation="MITRNLGEETINSIIEEDNLLSEQVQVIDGCIVINIEYEYPIPL
                     NTCSTYQQILHWVWHLAEKTWMTQIVTRR"
BASE COUNT         2509 a         2295 c         2336 g         2674 t
ORIGIN      
        1 aggtgttgca ttccacaatg ctcacggaaa agacgaatgg gtttgccttt ttgccgatag
       61 gagttgggtc ttagttcatt atgttgcagc ctgtcctctt ggatagaaat atatttatca
      121 agccattctg tcaccgtaat gtctgagcgc ctgcctttca ttctttccag acgctcattg
      181 acgcttaata tttgtctggt acgttgttca gcaataatgg tatttgcttc agtagcaact
      241 tgttttgctt cattctcatc agttcctaag ctatgaaaac gaccggatag tggatgtttg
      301 tattgccaat atacctttcc ggttcgctta tctaatttgc aatataaatt gggtatagag
      361 attttgtgag atcggggtct agcagccatc agcgattatc ctttggagtt ttgggtttgc
      421 gtttattggg agttgcggtt ctgcaagcgt tcctacaaaa cgggaatttc ggtcaatcat
      481 ccagtagcga ccaactttta tagcgggtgg ggccatcatt ttcccttgcg cgtatttttt
      541 cagaactcgc tcacttggtg ctaagtcccc aaattcttct ttagcccagt cctgtaaagt
      601 gattagtcga gacatttgtc ctcctcttag ctgctgaggg agtttgtgac cgatatatct
      661 gacatgatat taagctcatg gcaggtacat ctcttgactg gtcatagaga taaatttaat
      721 gctgagaaat gcagtattga atttatcaat ttttctattt cctgcgcatg tcacgtaact
      781 tcttaatgtg ttctgccgtt tcgatctctt ctgctatccg atctgcatca gctttattca
      841 caggttcaaa gtcatgatta aagcggaaca tgctggcgat acatgttctg ccttttcgga
      901 tgtagtgaac tttgttgtgg gtagaacgca ggattttgca gggagtgccg tggtggtcga
      961 cgtaccaggt gttagggtca atcactctga acattcttga ccttgtgttg tgcggattga
     1021 tattcagtgc tgatattcac ctttatagcg aacaccttta ccggtttatc gccaaagtgc
     1081 ggatgtgtga ttgtcttcac ttcatatccg tcatacggaa catcaattct gcgactggaa
     1141 tcgtcgcgct tcggatatcc ctttgtgata atcaggcggt catattcccg gaacataatt
     1201 cgcttattcc agtagtcatt acacaagcga tactcttccg ttttctctcc gcgaatcatg
     1261 gcatcgaagt attcactttt aacagcaagt tgcaggttag ccatcactta atcccccttt
     1321 gtttgcggat aagctccaga tcttgctggc aactggcgca agtccgacaa ccctgaacgg
     1381 ccaggcgtct tcgttcatct atcggatcgc cacactcaca acaatgagtg gcagatacag
     1441 ccaggtagtt caggcggcgc atttttattg ctgtgttgcg ctgtaattct tctatttctg
     1501 atgctgaatc aatgatgtct gccatctttc attaatccct gaactgttgg ttaatacgct
     1561 tgagggtgaa tgcgaacaat aaaaaaggag cctgtagctc cctgataatt ttgcttttca
     1621 tgttcatcgt tccttaaaga cgccgtttaa catgccgatt gccaggctta aatgagtcgg
     1681 tgtgaatccc ttcagcgtta ccgtttcgcg gtgcttcttc agtacgctac ggcaaatgtc
     1741 attgacgttt ttatccggaa actgctgtct gacttttttg atttcagaat tagcctgacg
     1801 ggcaatgctg cgaagggcgt tttcttgctg aggtgtcact gaacaagccc catgtcggca
     1861 agcataagca cacagaatat gaagcccgct gccagaaaaa tgcattcagt ggttgtcata
     1921 cctggtctct ctcatctgct tctgctttcg ccaccatcat ttccagcttt tgtgaaaggg
     1981 atgtggctaa cgtatgaaat tcttcgtctg tttctgctgg tattggcaca aacctgactc
     2041 caatttgagc aaggctatgt gccaaatcaa tactcgttct taactcaaca ggagatgctt
     2101 tgtgcatacc gccccccgtt tattatttat ctcctcagcc agccgctggg ctttcagcgg
     2161 atttcggata acagaaagac cgtgaaatac ccagcctcgc tttgtaacgg agtagacgaa
     2221 agtgatcgcg cctacccgga tattatcgtg aggatgggtc atcgccattg ctctccaaat
     2281 acaaaaccaa tttcagccag tgcttcgtcc attttttcga tgaactccgg caccatctcg
     2341 tcaaaactcg ccatgtactt ttcatcccgc tcaaccacga cataatgcag gccttcacgc
     2401 ttcattcgtg ggtcatagtt ggcaaagtac caggcatctt ttcgtgtcac ccacatgctg
     2461 tactgcacct gggccatgta agccgacttt atggcctcga aaccaccgag ccggaacttc
     2521 atgaaatccc gggaggtaaa cgggcatttc agctcaaggc cattgccgtc actgcataaa
     2581 ccatcgggag agcaggcggt acgaatactt tcgtcgcgat agatgatcgg ggattcagta
     2641 acattcacgc cggaagtgaa ttcaaacaga gttctggcgt cgttctcgta ctgttttccc
     2701 cattccagcg ccttagcatt aacttccgga gccacaccgg tgcaaacctc ggcaagcagg
     2761 gtgtggaagt aggacatttt catgtcaggc cagttctttc cggagcgggg ttttgctatc
     2821 acgttgtgaa cttctgaagc tgtgatgacg ccgagccgta atttgtgcca cgcatcatcc
     2881 ccctgctcga cagctctcac gtcgatcccg gtacgctgca ggataatgtc tggtgtcatg
     2941 ctgccacctt ctgctcagtg gctttctgtt tcaggaatcc aagagctttc actgcttcgg
     3001 cctgagtcag ttctgacgat gtgcgaatgt cgcggcgaaa tatctgggaa cagagcggca
     3061 ataagtcgtc atcccatgtt ttatccaagg cgatcagcag agtgttaatc tcctgcatgg
     3121 tttcatcgtt aaccggagtg atgtcgcgtt ccggctgacg ttctgcagtg tatgcggtat
     3181 tttcgacaat gcgctcggct tcatccttgt catagatacc cgcaaatccg aaggccagac
     3241 gggcacactg aatcatggct ttatgccgta acatccgttt gggatgcgac tgccacggtc
     3301 cggtgatttc tctgccttcg cgggttttga atggttcgcg gcggcattca tccatccact
     3361 cggtaacgca gatcggatga ttgcggtctt tgcggtaaat ccggcacgta catgattcat
     3421 tgtcctgctc aaagtccatg ccatcaaact gctggttttc attgatgatg cgggaccagc
     3481 catcaacgcc caccaccgga acaatgccgt tctgcttatc agggaaggcg taaatttctt
     3541 tcgtccacgg attaaggccg tactggttgg cgacgatcaa caatgcgatg aactgcgcat
     3601 cgctggcatc acctttaaat gccgtctggc gaagagtggt gatcagttcc tgtgggtcga
     3661 cagaatccat gccgacacgt tcagccagct tccctgccag cgttgcgagt gctgtactca
     3721 tccgttttat acctctgaat caatatcaac ctggtggtga gcaatggttt caaccatgta
     3781 ccggatgtgt tctgccatgc gctcctgaaa ctcaacatcg tcatcaaacg cacgggtaat
     3841 ggcttttttg ctggccccgt ggcgttgcaa atgatcgatg cagagtgatt caaacaggtg
     3901 ctgggacaga cccttttcca tgtcgtctgc cagttctgcc tctttctctt cacgggcgat
     3961 ctgctggtag tgacgtgccc agctctgagc ctcaagacga tcctgaatgt aataagcgtt
     4021 catggctgaa ctcctgaaaa tggctgtgaa aatatcgccc gcgaaatgcc cggctgatta
     4081 ggaaaacagg aaaggggatt agtgattgag gccgttaccg cgtccgtcga gaaaaacttc
     4141 cacgagcaaa tcacgggtat aagtgcgctc gatgccgcga tgcagataaa gccgtccgcg
     4201 taaattagct gatgcagtcc aggtaccatc tttgtgtttg accagcattc ctggcatgac
     4261 cgcacctcga ttaacggtct gcgttccgta atgttgatga accataaaaa ctcctgcccg
     4321 taagctgggc tgctgaacat atagagactt ctgcgcgtat tcaggcggtg gatggccgcc
     4381 ggttgtcata actaagccgc ctcgttgaag cgactgaggt atgaggtgtt gagttgattt
     4441 cagctggtca caccgacgtt cacgcgtccg cttcacccct cgcactcccc ggagcctgct
     4501 gaaattcaag ctgcggatct aagcggtcat cgcaacggtg aatcaggtga ttgccgtatc
     4561 gttgtgttgt tgcgacatgg tgataatagc tattgctatt ggctgtatca atacttattg
     4621 ctattgattg atgtgttttg atattaactg tttgatagca aaaagaatta attttgtgac
     4681 ttgcatcgca tagcgataac tgaagcgagg tcgtggtggt tttttgaacg gtttatgtga
     4741 tgaggggagg caaaagaaaa cccggcacga cggccgggga aatcatttcg catctacaat
     4801 aaataacctg tttatctggc cttttttaac agtagccttt gcggttatag tgaatactgc
     4861 agatggatca cctttggttg atatatatgc actaagagct ctaatatacg ggttattctt
     4921 ctttcctgca gctggatcgc taatttcagc agtgattctc ttttttgagt catcaccatc
     4981 taaaattatt ttagctgtca tattttgtgc atcaaattct gtaagaaaag cacgatactc
     5041 acgaagaccg agaacttcat catcatcaag cctatcaatt tcagctttat ctctctcgtt
     5101 aacttttaga aggcagccgt caacatttgt tgcaacactt atctgatcgc aagtattacc
     5161 aataggtgat actgcctgcc ttacagaggg gcgaagctct acagccattc ggtcaatcaa
     5221 agagatcaac ttatcaatgg ttccagcatc cttgtttcct agtgcctcta tggccttttc
     5281 aagtgactgc tgcaaagctt tcatttcatc tttcttgtta gaatttctcg caaaaatata
     5341 ttgtagtatt gcgccaagta tagttgcggc gatccccgag aacaactggt tctgagtggc
     5401 gaagttaaga actgcttcaa gagtaaagca gttagctttt gcttcgcgtg cgtaaacctt
     5461 aacttcctga taattaatgt atttactata tttttgtgta acagagaaag aagctgctgt
     5521 tgagagaact ttagaaaaac cctttaggga ttctcctagg cagttcaaat ctatttcatg
     5581 atttaaagca tcttttccgt catacctaag agagattttt atatcctgta aagcgtcaca
     5641 atccataaat cgctctcgtc taattctaat taaatttact atcccctaaa cgactcatca
     5701 gcacagtact gattatccat gtttcctgta cgtctggggc atgctcccaa taaccttacc
     5761 gaagatgaac acccggttca tctcgtcttt ctcgatcggg tcccacggtg agtagctctt
     5821 gttatcagag ataaccagca gcttatcctt catcatttgc aggcgcttta catgggctgt
     5881 gtcgtcgtac agaaacgcat agataccatc accgtcgaaa gatttaaccg tgatatcaac
     5941 gaacagcaga tcacctggtt cgatcgttcc tgacatgctg tcaccacgca cgttaatgat
     6001 gcggatattt tccgccttcc tgccatcgaa catgtgacga gcatcgtcaa acgagtactc
     6061 aaccgagcgt aggacttcta caaactcacg gttgatgact cccggcccgg cactcacttc
     6121 tatatcaaga acgtcaatct tgaagtattt ggaatggttg acagtaggct tccctgattg
     6181 ttgaccgtca tttctcatcg ggcctatgcc tgatgagagc cactctgttc gaacacccaa
     6241 tgcattagct atttcaacaa tttttgttga gccgcgcgcg ttgccgcttg tcagtctcca
     6301 gattgtgggt tgagctacgc cagacgcctt tgcaagagcg ccttgagaca tcccagattg
     6361 ttccatcgct aggtttaagc gatcagcaag agtttctttt ttcataagtt ttaatttata
     6421 cgcttgcgta ttgatggtca aaacacgttt tgctattgct acggttaata cgcattgcta
     6481 ttattcattc attgtaatac caataggaat tgataatgac aaatcaaacc attcaactcg
     6541 caatcagtat tacaggtagt caaaaacgac tggcagatct atgcggtgta gcccagccca
     6601 ctgtttggcg ttggctacac ggtggcggaa ttgatgcccg ctatgtaatg aaaattgtct
     6661 cagccactgg tggaaagatt aagccagcag atattcgtcc cgacctcgca ccattgttta
     6721 acgcgagtaa ttctgccgcc taatctgcgg cgttaactga taaggcgatg attatgcaac
     6781 cacttacata ccaacagact agcggattta gcccgactgc ggtgataaat cgttctcaaa
     6841 taaaacaggt gccaggccac gaaaaaattc gtgatgccgt ccgcgcctgg tctgctgaag
     6901 ataatcagga tgtagttgcc gcactcattg tgaatgagta tcgagcacag ggcggcggca
     6961 ccatcgattt tcctgatgat gtcagtcgtg cacgccagaa gctgttccgc ttcctcgata
     7021 acaaattcga ttctgaaaaa taccgaaata acgtgcgtga actgaccccg gcaattctgg
     7081 cggtactacc gctggaatat cgcggccacc tggttgagca ggatagcttc atggctcggc
     7141 tggctgaaat ggaaaaggaa ctcagtgagg caaagcaggc ggtcattctc aatgcaccac
     7201 gccaccagaa actgaaggag atgagtgaag gcattgtgtc gatgtttcga gtggacccgg
     7261 atctggctgg tccattgatg gcgatggtca ccaccatgct gggggcaata tgacaggttc
     7321 aaaaatggcg aaagtcggtc tgcgggaaca gaaccgactt tcaggtgcaa atcgtaacac
     7381 actcattgcg ggaggaatta tggcaaacac tgctgagata ttcaattttc cagtgccgga
     7441 tgcggcacaa aaggagccgc gcgtggcaga tctcgatgat ggttatacgc gcattgcaaa
     7501 tgagttgctg gaagctgtaa tgctggccgg attaacacag caccagcttc tggtcttcct
     7561 ggctgtcatg cgcaaaacat atggctttaa taaaaaactg gattgggtga gcaacgagca
     7621 actttccgag ttgaccggga tattgccgca caagtgttct gctgcaaaaa gtgttctggt
     7681 aaagcgtggg atttttattc agagcgggcg gaatatcggc attaataatg tggtcagtga
     7741 atggtcaaca ttacccgaat caggtaagaa aaataaagtt tacctgaaag aggtaaattt
     7801 acctgaatca ggtaagaaaa gtttacccaa atcaggtaaa ggcacttacc cgaatcaggt
     7861 aaacacaaaa gacaaactaa caaaagacaa tataaaacct ttttcgtccg agaattctgg
     7921 cgaatcctct gaccaaccag aaaacgatct tcctgtggtg aaaccagatg ctgcaattca
     7981 gagcggcagc aagtggggga cagcagaaga cctgaccgcc gcagagtgga tgtttgacat
     8041 ggtgaagacc atcgcgccat cagccagaaa accgaatttt gcagggtggg ctaacgatat
     8101 ccgcctgatg cgtgaacgtg acggacgtaa ccaccgcgac atgtgcgtgc tgttccgctg
     8161 ggcatgccag gacaacttct ggtccggtaa cgtgctaagt ccggccaaac tccgcgacaa
     8221 gtggacccaa ctcgaaatca accgtaacaa gcaacaggca ggcatgatag ccagcaaacc
     8281 aaaactcgac ctgacaaaca ctgactggat ttacggggtg gatttatgaa aaacatcgcc
     8341 gcacagatgg ttaactttga ccgtgagcag atgcgtcgga tcgccaacaa catgccggaa
     8401 cagtacgacg aaaagccgca ggtacagcag gtagcgcaga tcatcaacgg tgtgttcagc
     8461 cagttactgg caactttccc ggcgagcctg gctaagcgtg accagaatga actgaacgaa
     8521 atccgccgcc agtgggtgct ggcttttcgg gaaaacggga tcaccacaat ggaacaggtt
     8581 aacgctggaa tgcgcgtagc ccgtcggcag aatcgaccat tcctgccatc acccgggcag
     8641 tttgtcgcct ggtgccggga agaagcatcc gttaccgccg ggctgccaaa cgccagcgag
     8701 ctggttgata tggtttacga gtattgccgg aagcgtggcc tgtatccgga cgcagagtct
     8761 tatccatgga aatcgaacgc gcactactgg ctggttacca acctgtacca gaacatgcga
     8821 gccaatgcgc tgactgacgc ggaattacgg cgcaaggctg ccgatgaact gacctgtatg
     8881 acagcgcgaa ttaaccgtgg tgaggctata cctgaaccag taaaacaaat tcctgtcatg
     8941 ggcggtagac ctctaaatcg tgcacaggct ctggcgaaga tcgcagaaat caaagctaag
     9001 tttgggctga aaggagcaag ggtatgacgg gcaaagaggc aattattcat tacctgggga
     9061 ctcataagaa cttctgtgcg caggacgttg ccgcggtaac aggcgcaacc gtaaccagca
     9121 taaatcaggc tgcggctaaa atggcgcggg caggaatcct ggtcgttgat ggtaaggtct
     9181 ggcgaacggt gtattatcgg ttcgctacca gagaagaacg ggaaggaaag atgagcacga
     9241 acctgatttt taaggagtgt cgccagagtg ccgcgatgaa gcgagtgttg gcgatgtatg
     9301 ggcgaggatg agtgatggaa cggggcttga cctgtatgct gtccgtgtac ttgcaaggaa
     9361 atttagatgc agtacgagat gagtccatca aaaataaact caaaaatcaa agtgagtagg
     9421 tacatcgttt gcaatcgaca atgccctata atagatgttg aataattgca taaaatgatg
     9481 ctatacctct tttgccttat tcatattgag tttaattctt ctcaacagaa aggacttatc
     9541 gacgcacgat aagtgtcata tatgcaattg tttatcaaca caaaatctga ggaattatga
     9601 ttactagaaa tcttggcgaa gaaacaatca atagtattat cgaagaagat aatctgctgt
     9661 cagagcaggt tcaagtcatt gatggttgca tcgttataaa tattgaatat gagtacccca
     9721 ttcccttgaa tacatgctca acttatcaac aaattttgca ttgggtatgg cacttggcgg
     9781 aaaaaacatg gatgacgcaa attgtcactc gtcg
//
LOCUS       DADTFG010000168         9805 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.169, whole genome shotgun sequence.
ACCESSION   DADTFG010000168 DADTFG010000000
VERSION     DADTFG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.169"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            156..806
                     /gene="csgD"
                     /locus_tag="J1138_004024"
     CDS             156..806
                     /gene="csgD"
                     /locus_tag="J1138_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBA8455758.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            811..1200
                     /gene="csgE"
                     /locus_tag="J1138_004025"
     CDS             811..1200
                     /gene="csgE"
                     /locus_tag="J1138_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBA8455759.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1225..1641
                     /gene="csgF"
                     /locus_tag="J1138_004026"
     CDS             1225..1641
                     /gene="csgF"
                     /locus_tag="J1138_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBA8455760.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1668..2501
                     /gene="csgG"
                     /locus_tag="J1138_004027"
     CDS             1668..2501
                     /gene="csgG"
                     /locus_tag="J1138_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBA8455761.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2565..3056)
                     /locus_tag="J1138_004028"
     CDS             complement(2565..3056)
                     /locus_tag="J1138_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBA8455762.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(3158..3712)
                     /locus_tag="J1138_004029"
     CDS             complement(3158..3712)
                     /locus_tag="J1138_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA8455763.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3736..4473)
                     /locus_tag="J1138_004030"
     CDS             complement(3736..4473)
                     /locus_tag="J1138_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBA8455764.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4528..5466)
                     /gene="ghrA"
                     /locus_tag="J1138_004031"
     CDS             complement(4528..5466)
                     /gene="ghrA"
                     /locus_tag="J1138_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBA8455765.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQTRVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            5699..5786
                     /locus_tag="J1138_004032"
     tRNA            5699..5786
                     /locus_tag="J1138_004032"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5733..5735,aa:Ser,seq:gga)
     gene            complement(5936..6779)
                     /locus_tag="J1138_004033"
                     /pseudo
     CDS             complement(5936..6779)
                     /locus_tag="J1138_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6876..7073)
                     /locus_tag="J1138_004034"
     CDS             complement(6876..7073)
                     /locus_tag="J1138_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBA8455766.1"
                     /translation="MKSLTTETALDILITWLQDNIDRESGIIFDNDEDKTDSAALLPC
                     IEQAREGIRTLRQLQLLHQNR"
     gene            complement(7070..7573)
                     /locus_tag="J1138_004035"
     CDS             complement(7070..7573)
                     /locus_tag="J1138_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455767.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSMSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAEYLPGLPTF
                     DRSPGTT"
     gene            complement(7570..7947)
                     /locus_tag="J1138_004036"
     CDS             complement(7570..7947)
                     /locus_tag="J1138_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBA8455768.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMVRDNYRTVNNITLGKHPGAKQ"
     gene            complement(7994..8368)
                     /locus_tag="J1138_004037"
     CDS             complement(7994..8368)
                     /locus_tag="J1138_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBA8455769.1"
                     /translation="MSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            complement(8418..9062)
                     /locus_tag="J1138_004038"
     CDS             complement(8418..9062)
                     /locus_tag="J1138_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HBA8455770.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQALT
                     ADAEAAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEAIHINAYTFTEAGLHAG"
     gene            complement(9081..9302)
                     /locus_tag="J1138_004039"
     CDS             complement(9081..9302)
                     /locus_tag="J1138_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBA8455771.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTETYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(9365..>9805)
                     /locus_tag="J1138_004040"
     CDS             complement(9365..>9805)
                     /locus_tag="J1138_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBA8455772.1"
                     /translation="TRERSLILRALKTLDRHLHEPGVAFTSTRAAREWLILNMAGLER
                     EEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALHHNAAAVVLAHNHPSGEV
                     TPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         2462 a         2436 c         2431 g         2476 t
ORIGIN      
        1 ttgaaatcag atgtaatcca ttagttttat attttaccca tttagggctg atttattact
       61 acacacagca gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc
      121 aggtgtgcga tcaataaaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
      181 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
      241 accttaaaca atcgctggca attacaggaa aattacataa tattcaacgt tctctggacg
      301 atatatcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
      361 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
      421 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
      481 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgcggcgaat
      541 gctactttac gcaaaagctt gccagttacc tgattacgca ttcaggtaac tatcgttata
      601 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
      661 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
      721 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
      781 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
      841 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
      901 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
      961 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc gcgatgggga
     1021 agctggatca caataacggt gaatcaggac gttattttcc agactttttt atttccgttg
     1081 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     1141 cgtcgccaga taaaccaggc gttattaagt acgggcgatt tggcgcatga tgaattttaa
     1201 ataataaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     1261 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     1321 ggtaacccaa ataacggtgc ttttttatta aatagcgccc aggcacaaaa ctcttataaa
     1381 gatccgagct ataacgatga ctttggtatt gaaacgccat cagcgttaga taactttact
     1441 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     1501 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     1561 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     1621 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     1681 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     1741 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     1801 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     1861 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccacggca atgctggtca
     1921 cggcattgaa agattctcgc tggtttatac cgctggagcg ccagggctta caaaatctgc
     1981 ttaacgagcg caagattatt cgtgcggcac aagaaaacgg cacagttgcc attaataacc
     2041 gaatcccgct gcaatcttta acggcggcaa atatcatggt tgaaggttcg attatcggtt
     2101 atgaaagcaa cgtcaaatcg ggcggggttg gggcaagata ttttgggatc ggtgccgaca
     2161 cgcaatacca gctcgatcag attgccgtga acctgcgcgt cgtcaatgtg agtaccggtg
     2221 agatcctctc ctcggtgaac accagtaaga cgatactttc ttatgaagtt caggccgggg
     2281 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcgaacg
     2341 aacctgttat gctgtgcctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     2401 atggtatcga ccgtggtctg tgggatttgc aaaataaagc agaacgacag aatgacattc
     2461 tggtgaaata ccgccatatg tcggttccac cggaatcctg atagataaaa aagccgaaat
     2521 tatcatccgg cattttacgg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     2581 atttgctgct cacggtgagc cgtctttgca ctacgcgccg ccaaccacag gccgctgttt
     2641 ttcattgcgt aaccaaatac cagtccaagc gccagcgaag gtaacaccag tttccagtca
     2701 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     2761 agctgtttgg cctggataca catcagaaac gcgacaatac cggttatgac ataaccgagt
     2821 atttccagat gtggtgccaa tgcactaccg taaataatga ccatcgccca caccacgcca
     2881 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaggcaaag
     2941 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     3001 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     3061 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     3121 aattacccgt tgcagttatg agtgatgcag atcacattta ctcttcagaa tcttcttcca
     3181 gctcgtccca cattgcacta atagcatcgc gggttagcgg tgccatggtg cgccagaaag
     3241 gcgtagttgc atgggcctcc actttgccaa ggaacgcacc acaccagggt aacagatact
     3301 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     3361 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgctagt ggcatccctc
     3421 gttcggaaag aaaagcgcgc acttccgctt ccgttgcgcc ctcaacccat gcgctacgat
     3481 atggcggcac agcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     3541 tcatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     3601 cagccagttt cccctcacga atcagggtaa acaacggcac cagtaaagga tcttgcggtt
     3661 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcattc attaatatat
     3721 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     3781 aaggaagttc agtaaacggc gtggcgaaac attcaaaatg cgctctggcg gaaaatctac
     3841 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcag tgtgagaatc
     3901 cgagcctaat gccacccaac ctccagcgtc acgtaccgct gcggctaccg cacgacagtt
     3961 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     4021 atgtttcgcg gctgcttcag caacggcttt aacatctatc tcatatttcg gatttccggg
     4081 atggcttatt atatgcacat tgccgctggc gatagtggcg atcatcgctt gtgtattggt
     4141 cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     4201 atcaaacatt ttgccgctgc agtcaatttc accatcaaca tttttaatgt tagcttcgat
     4261 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     4321 ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     4381 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gcgtgctggc
     4441 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     4501 aaggatagca ggaaacctga tgctttatta gtagccgcgt gcgcggtcga cttgcccgca
     4561 gaccctctcc cctttttcga gctgggcaat ggtgcgcgaa atgtaatcca cagcttcagc
     4621 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     4681 actttccggc ggtaagggtt cacgattaaa aacatccagc attgcgcctt taactttgcc
     4741 gctatccagc gccgcgagca ggtcatcttc cacgacatga acaccacgcg ccaggttgag
     4801 gagatacgcg ccatccggta atttttcgag taattgttga ttaataatgc cgacggtttc
     4861 aggtgtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagagagttc
     4921 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     4981 cagcgggaag cgccaggttt gcagactctg tgcaacttta ctgcccagta cgcctgcgcc
     5041 caaaatgccg atggtaaaat cttcccgatg atattcagga agcggttgcc aatgcgaact
     5101 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     5161 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     5221 cttcagcatt tcagggtgag cctgtagctt gctcaaaata gaatcaacac ctgccccaag
     5281 tgcaaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     5341 agcataatcg gctgattcat tgtctccgct tttccatgct ctgactcttg tctgaggaat
     5401 agctttgtgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     5461 atccatactg actcccgaag agtgttggcg aaatttattc gcacttatcg ttatgatcta
     5521 caaagaccac cagcataaca aatccgtggt cggtggcaaa aaaagcaaat ttcgcttatt
     5581 aaaactgcat attgattgaa gtttgaataa tcgagcgatt ttttaaaaaa gtttgttgac
     5641 ctcaggtcat gatttcccta aattagcgac cgttccagca agacagggac gacaatttgg
     5701 tgaggtgtcc gagtggctga aggagcacgc ctggaaagtg tgtatacggc aacgtatcgg
     5761 gggttcgaat ccccccctca ccgccagatt acaagcgcat gttattgttt aacatgcgct
     5821 tttttattta tccttccaga cagtatgcca ccgggtatgc catctgaaac cggtttatat
     5881 gccccacgat catatcttat cggacaattt ccgagctttg agcatactgg caaagctata
     5941 ctgccagcat gtcaggatag tgtttttgcg ataatatcat tcagtctgtc aaccagttct
     6001 ggcttcctga acgtgatgcg cgcagagccc ttcttaaagt atttaataat gaacatctca
     6061 tcttcatagc attcttttcc ctgaagagac cggatgtgat cgtccagatg aatagttatg
     6121 ttttccctgt tgtcggggat cggtttgccg ctgaacagat gcagcattct ttccaggtcg
     6181 gcaagtctat ctgcctgttg cccggtgata agatgcaatc cccaccggtc ccacctcatc
     6241 agattgttga cgataatttt attgccaaat ttgcaggggc aattggtttt gtaattccag
     6301 ctcaaccctc tgaagaggtt gatcactcct cgctcaaaca cttcatcctt attctggtgt
     6361 agctgctcaa acgtactcag aatattctct tcactgattt ccggaaaatt atcgtactcc
     6421 agtgatcggt accatgtatc atgggtctgc gcatccatta atgacagcat cccggagcgt
     6481 tgcatcaggt cacgccagat ttcacggtca aggttgcggg taatggcttt cattgcggtt
     6541 tccggtttct cagtcagcca gcaaccacag cgaaaatcct gtttcatggc ccagtccagt
     6601 gcggttttac cgccaatact ttctgtcatt gatgagattt cagccagttg ccggataagt
     6661 gtgtcgattt gcagcagtgt actctttcgt acagccagaa tgtggcggat actggttgag
     6721 cagataacgt ccgtatgccc gttggccact tccggacgag agactgtgat tgcagacata
     6781 atcgatattc cttttcgcag ataaatgaaa acgccagccc tgttcaggct ggcgtcagtg
     6841 gaatgaagtc tggtgagtga gataatgagt aagactcacc ggttctggtg cagaagctgc
     6901 agttggcgca gggtacggat accctccctg gcctgttcga tacaaggcaa cagtgctgcc
     6961 gaatcagttt tatcctcatc gttgtcgaag ataattcccg attcgcggtc gatattgtcc
     7021 tgcagccacg taatcagaat atccagcgct gtttccgtag ttaatgattt catgttgttc
     7081 ctggtgacct gtcgaaagtg ggcaggcccg gcagatattc agcgctggca tccagatgaa
     7141 tgagactgac gccataacgt cgggtaagtg tgacaaccag acgacggaac gtttttgaca
     7201 gccccaggcg tttaagccgc agaaccgggt atgaccacgc atcggtacgt aacagatatc
     7261 cgctgcctgt aaaatgaatc cattctgatt cgccggtgtc attcgtctga tgcgacatcg
     7321 agtacagcca ggcgctgtct tttgccgtga tatgcgcggt actgcagcgt atgccggtca
     7381 gggcagcaaa tggtggcgtg cagtctgttg aggcccggtc agattcatca acaacacgaa
     7441 gggagtgacc gttatcgcag accacgttaa tcagttcagc aagctcaata ccatcgacgt
     7501 caacgacaat gcgccccatg ttcagtgcct gcacgttaat gctgtcggct tccagcgtca
     7561 gggccagttt cattgtttcg cccccggatg tttacccagg gtaatgttat ttaccgttct
     7621 gtaattgtcg cggaccatca ggccggttgc ccggcgggcc cggaggatat caatactgtt
     7681 tattaactga gaacgggtac aagcgctgaa tcctggctgg tcggtacgta ccagtgcgta
     7741 tttttcaacg agaaagttca ccgcatcgca cagtgaaatg cctgcctcaa tatgctgctc
     7801 aatcacacgt tcatcagcga acggtgtgtc attcagtgtg aggccgtaat gctggtccaa
     7861 cagttgggtg agcagtgtct gccagatggt gacgggagac gggcagcgcg atgcctcccg
     7921 tacgtgtgtg tcaggtaatg ttttcatctt aagttctctg taactggtag tgctaaaggt
     7981 gagatattcg gggttatgag gtggttgcgg gtgccagtgt cggataaaca gccatataaa
     8041 cgtaaccaca gctgccgagg gtgtcggctt cgcaggtcag ccctctggca tacagcgtga
     8101 cggtatgctg atggcgggga ttcagttcac cgctggtgag catgagttcc agttgtttca
     8161 tcagcagtgg aaaagcctgg tccagatggt atgcatccgc gtcgctgaac tgccctctga
     8221 taccggcgcg gtcggcaagg taatgcaact gattgccctc ctgcaccaga cgtgccccga
     8281 aacagggcgt cacggtgcag ggtagccccc accaggggcg gtcgttgttg tcgtcgggat
     8341 aatttgtttc atggagcgtg tctgacacga taaagtcctc acagaaaatc ggtgaatgat
     8401 agtttaacga tgacaggtca gccggcgtgg agaccggctt cggtgaaggt atatgcattg
     8461 atgtgaatgg cctcatctac agcggcgtct gaggtcagcc agtcgtactc gttttccagc
     8521 tggcggtaca gccagaaggc caggtcgcgc agggcttcgc tgacagcggc ttccgcatcg
     8581 gctgtcagtg cctgaccagt cggactgttc cgggtgacgg tgatatccat gctgtacgga
     8641 tggcagtaac gtccccggtg actgatttca gcctgtaact gccagaagtt acgcttctgt
     8701 accgcctgca gggcatccgc gatacgatga agctcacggt cctgaggggc atattccctg
     8761 attttccggg gtgtcgctgg ctgccagtgc cagcgccctt caaaacaggc tccgtccccc
     8821 tggctgcaga atccgctgaa ccagatgcac ggctcttcca taaacctgcc gttgctgagc
     8881 cggaaaacgc gctggcggag gtcaattccg aaaatgccac agacctccct gaagtcctca
     8941 tacacgtttt cataccagtt gctgtcagca tgatgctgac ggtaccagtc gcgggctttt
     9001 tcctgtgcgg tggaggatag ctcatgcagt gtatacacgg tggtggtgat aattctgctc
     9061 atcagaatgc ccttactgac tcagttcaga gtgacgctca tcagccggac atacgggcca
     9121 aaactgtcct tacggcgttc agcaaacacg gccagcacac cgggaatatc ctgcacttca
     9181 cgaccggtat acgtttcagt gctgccgtgc cagcggtatt tgccggtaca gaacggaaaa
     9241 agacgggatg caggatgctg ttggtgaata cgcatggctt caccacgggt gatgattttc
     9301 ataatgggat acctctgaag acagaagata aaagtgaaaa caggtgtgat gtggttgtga
     9361 cgggttaaag cagaccatgt tcggcaaagg agaaaacctg gctgccaccg actatcagat
     9421 ggtccggcac ccggatatcc accaggccca gtgcctgtac cagacgttcc gtgataagcc
     9481 ggtctgcctt gctgggtgtg acttcaccgg acgggtgatt atgtgccagc accacggctg
     9541 cggcattgtg gtgcagggcg cgtttaatca cttcccgggg atggacttcc gtgcggttga
     9601 tggtgccggt gaagagggtt tcaccggcaa tcagctgatt ctggttgttc agatacagca
     9661 cccggaactc ttcacgttcc agtcccgcca tgttcagaat cagccattcc cgtgccgcac
     9721 gggtggaggt gaaggccacg ccgggttcat gaagatggcg gtccagggtt ttcagggccc
     9781 gcagaatgag gctgcgctct ctggt
//
LOCUS       DADTFG010000169         9799 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.170, whole genome shotgun sequence.
ACCESSION   DADTFG010000169 DADTFG010000000
VERSION     DADTFG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.170"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            37..651
                     /gene="evgA"
                     /locus_tag="J1138_004041"
     CDS             37..651
                     /gene="evgA"
                     /locus_tag="J1138_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBA8455773.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            656..4249
                     /gene="evgS"
                     /locus_tag="J1138_004042"
     CDS             656..4249
                     /gene="evgS"
                     /locus_tag="J1138_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBA8455774.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEVFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FAAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(4305..5450)
                     /gene="yfdE"
                     /locus_tag="J1138_004043"
     CDS             complement(4305..5450)
                     /gene="yfdE"
                     /locus_tag="J1138_004043"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBA8455775.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5524..6468)
                     /gene="yfdV"
                     /locus_tag="J1138_004044"
     CDS             complement(5524..6468)
                     /gene="yfdV"
                     /locus_tag="J1138_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBA8455776.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6538..8232)
                     /gene="oxc"
                     /locus_tag="J1138_004045"
     CDS             complement(6538..8232)
                     /gene="oxc"
                     /locus_tag="J1138_004045"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBA8455777.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALGAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8286..9536)
                     /gene="frc"
                     /locus_tag="J1138_004046"
     CDS             complement(8286..9536)
                     /gene="frc"
                     /locus_tag="J1138_004046"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBA8455778.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPSVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2899 a         2238 c         2013 g         2649 t
ORIGIN      
        1 gagaagggaa atgcttcatt gcaaagggaa taatctatga acgcaataat tattgatgac
       61 catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat tgaaatctta
      121 gcagagttga ctgaaggcgg aagcgccgtt cagcgggtgg aaacacttaa gcctgatatc
      181 gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga aacgctgaga
      241 aagcgccaat atagcggaat tattattatt gtctccgcta aaaatgacca tttttacggg
      301 aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg catgaacaat
      361 atcattgcag ctattgaagc tgcaaaaaat ggctactgct atttcccttt ctctctcaac
      421 cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc gaaacaagaa
      481 attagtgtca tgcggtatat tcttgatggc aaggataata atgacattgc tgaaaaaatg
      541 ttcatcagca acaaaactgt cagcacttat aaaagtcgct tgatggaaaa attagaatgc
      601 aaatcactga tggatcttta cacattcgca caacgtaaca aaatcggcta accacatgaa
      661 gtttttaccc tatatttttc ttctctgttg tggtctttgg tcgaccataa gcttcgcaga
      721 cggagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac ttgatccact
      781 acgtctgagc aacaaggaat tacgttggct cgcgagcaaa aaaaatcttg tgattgcagt
      841 acacaagtcc caaacagcta cgttgttgca taccgattcg cagcaacgga ttcgtggtat
      901 taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaattaa cactccggga
      961 atacgcagat catcaaaaag caatggacgc gctggaagac ggtgaagttg atatagtgtt
     1021 atcacattta gttgcttcgc cgcctcttaa tgatgacatt gctgcaacta atcctctgat
     1081 aattaccttt ccggcgctgg tcactaccct tcacgattca atgcgaccgc ttacctcatc
     1141 aaaaccagta aatattgctc gagtagcaaa ttatccccca gacgaggtaa ttcatcaatc
     1201 atttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag catccgtctc
     1261 agccggacag aatgattact ttattggtag taacatcatt accagcagta tgatttcccg
     1321 ttattttact cactccttaa atgtagtgaa gtattataac tcaccgcgtc aatataactt
     1381 tttattaacc agaaaagatt caatcgttct taatgaagta ctcaatcgat ttgttgatgc
     1441 tttaacaaat gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc
     1501 ctttctgaac aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc
     1561 cgatttaaag gtgctggaaa atccttactc gccaccctat tctatgacag atgaaactgg
     1621 ctcagttcga ggcgtgatgg gtgatattct taatattatt accttgcaaa caggtttaaa
     1681 tttttctccg atcaccgttt cacacaatat tcatgctggg acacagctta accccggtgg
     1741 atgggatata ttgcccgccg ctatttatag tgaagatcga gaaaataatg tttcatttgc
     1801 tgaagtcttc ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca
     1861 aacattaaaa aaaggaatga aagttgccat tccatattat tatgaacttc attcgcaatt
     1921 aaaagagatg tatccggagg tagagtggat aaaagtcgat aacgccagcg ctgcatttca
     1981 caaggtcaag gaaggtgaac ttgatgctct ggtcgcgaca cagttaaatt cgcgttacat
     2041 gatcgaccat tactatccta atgaacttta tcattttctt attcccggcg ttcagaatgc
     2101 atcactttcg tttgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc
     2161 actgaatgca attcccccaa gcgaagttct gcgcctgacc gaaaaatgga ttaaaatgcc
     2221 caatgtgacc attgacacat gggacctcta tagcgagcaa ttttatattg ttacaacatt
     2281 atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg
     2341 tcgtcgtaaa gttattcagg gtgatttaga aaaccaaata tcattccgga aagcgctctc
     2401 ggattcctta ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcacaa
     2461 tagtgcattt gaacattatt tcactgctga ttactataaa aatgcaatgt taccattaga
     2521 aaatagtgaa tcccccttta aagatgtttt ttctaatacg catgaagtca cagcagaaac
     2581 gaaagaaaac cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag
     2641 atgcattaat cactggcata ccttatgtaa tctgccagca agcgaacatg ctgtttatat
     2701 ttgtggttgg caagatatta ccgagacgcg tgatttaatt catgcactcg aagtagagag
     2761 aaataaagcg atcaatgcaa ctgtcgcaaa aagccagttt ctggcaacaa tgagtcacga
     2821 aataagaaca ccaataagct ccattatggg cttcctggaa ctactgtcgg gttctggtct
     2881 tagcaaggaa caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct
     2941 cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca
     3001 accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt tcgctgcgat
     3061 tgctgcaagc aaatcgatcg cattaagttg cagcagtacg cttcctgaac gctacctggt
     3121 taagatcgac cctcaggcat ttaagcaggt cttatctaat ttactgagta atgctctcaa
     3181 atttactacc gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca
     3241 cgctgttatc aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca
     3301 acaactgttt aaacgctata gccaaacaag tgcaggtcgt cagcaaacag gttctggttt
     3361 aggcttaatg atctgcaaag aattaattaa aaacatgcag ggcgatttgt cattagaaag
     3421 tcatccaggc ataggaacga tatttacgat cacaatcccg gtagaaatta cccaacaagt
     3481 ggcggctgtc gaggcaaaag cagaacaacc tataacacta cctgaaaagt tgagcatatt
     3541 aatcgcggat gatcatccga ccaataggct attactcaaa cgccagctaa atctattagg
     3601 atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca
     3661 ttatgatctg cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg
     3721 caaactccgt gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc
     3781 taacgaacgt gaaaaagggt taaattgcgg catgaactta tgtttgttca aaccgttgac
     3841 tctggatgta ctgaaaacac atttaagtca gttacaccag gttgcgcata ttgcacctca
     3901 gtatcgccac cttgatatcg aggccctgaa gaataatacg gcgaatgatc tacaactgat
     3961 gcaggagatt ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca
     4021 tgcactagaa gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc
     4081 ggctaacatc ctgaatttgc aaaagttgat taatattagc caccagttag aaataacacc
     4141 tgtttcagat gacagtaagc ctgaaattct tcagttgctg aactctgtaa aagaacacat
     4201 tgcagagctg gaccaggaga ttactgtttt ctgtcagcaa aatgactaaa tagcggctcc
     4261 cacaatgttc aaatgtggga gctatttacc agcacatctt acatttatga tgagaactcc
     4321 tggcgaattt gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc
     4381 gcgcagccgc tgatttttat cggattaccc ggcatcatta ttcccccggc ttcaatcaac
     4441 atatttctcg cctgagtttg tggtaaatta atggcctcag ccacacttaa taacggcgcg
     4501 acgggcacac caacttcatg tattctggct aaccaaactt cagctacctg cgtttttaac
     4561 gtccgctcaa tatattcttt aagaatagcc tggttatgta cgcgtaaaat attgctgcta
     4621 aatcggggat cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc
     4681 ttgtcattac cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt
     4741 gccatgtagg gatggcgatt tcccaggcgt tgtggtgact tcccagtcgc gatatatgcc
     4801 atcagtccat gctccagaaa actcagagtg gcatcaaaca tcgctatatc gacatgcgcc
     4861 cctctctggc tcttttcgcg gccataaagt gcactcacta ttccactgaa taaataaaca
     4921 ccgccacaga gatccgcaag agaggtacca acgcgcactg gcggagcatc agggtatcct
     4981 gtttccatca ttatcccgct cattgcctga atgatggtat cgtaggcagg agcatctttt
     5041 agcggaccgg tatgtccgaa acctgacgat gaagcatata taaggcgcgg gttgatttct
     5101 tgtaaccttt cccatgaaaa cccgagtttt tccattgtac ctgggcgaaa attctcagct
     5161 aatacatcag cttgtttaag catatttata aatatacttt tatcgtgatc attctttaaa
     5221 tcaagaacca cactctcttt tccatgatta ataaaactgt aatagagcga ctgtccatcc
     5281 acatagggac caaatgtgcg ggtatcatca ccatgacccg gcggctcaac tttaattacc
     5341 cttgccccca tattacaaag aagttgagtt ccgaaaggcc cattaaggac atgcgtcata
     5401 tcgataacta ataagccttc aaacggccct ttgcattcat tatttgtcat ttgcttatgc
     5461 cttgaaaaaa gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat
     5521 taattatgaa accagtcgac tgacataaat ccacaaagga gccgtgacga caaaacccag
     5581 tacgctcaca gccaatgacg ctgtaccagt gcgggtgtag acattaaacc ggctggcaat
     5641 aatgattccg gagaatgccg gcggtaatgc gcctgccagt accatcatct gcaaatgttc
     5701 gctattcaaa tgacatgcca tacctacgag aagcagcgcc agtggcatca gaatcagctt
     5761 caggaaggtg ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa
     5821 ccccgcagcg aataccgcca cccctgagtt agccttcgca atcagattaa aggttgggtc
     5881 ccatgctgcc ggaattttta ccccaaccaa caccaggatc gttgccagaa caggtgccca
     5941 tactactggc tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc
     6001 cgctcctgag gaaggattca gcaaatacag accaatagga atagtaattg cgttaacaat
     6061 aatagaaata attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag
     6121 aactgcaaac cccaggaatc caatggtagg tgaacctgca attaatgcac atactgctgc
     6181 ttctgcatgg gtacgtttaa aaaatttgta gcaaccgaac caggagaaaa agaaacatcc
     6241 gacaataacc acaagtgata caagggtcag gcgagtgtcc gcaaaaatca tttccctgtt
     6301 tgcccgggta atagaaacaa atagagccgc aggaagagcg tagtttaata ccagtttatt
     6361 aaatgcccga gcttgatcct ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag
     6421 catgattacg ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc
     6481 gataagggtc cccttaccat cgcataataa gttggcaaga ggagacgatg tcagtattta
     6541 attaccagcg acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc
     6601 tgggtcgatg accacattaa taatggtcgg tttgcgcgac tgaattccgg tggttaaagc
     6661 atgacgaagt tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg catccattaa
     6721 tttgtcatac cttgcatggt gcaacagatc cgttggcgat ggtgcaccag cgccgctgag
     6781 gtcaacaccg tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag
     6841 gttatatcga caaatcgttt caatctccat cccactgaaa ccaaaagcac tatcaccttc
     6901 aatggcgaca accggagaac cagaggtcac gctagcaccg atggcatagc ccataccgat
     6961 gcccatgaca ccccaggtgc cacaatccag acgacgacgt ggtttataca tatcaataat
     7021 atttcgtgca ttatccaggg tatttgcccc ttcattaact aaataaatat cctggttctc
     7081 gcgcaaaaca tcgcgcacgg cacccaatgc attaaagtaa tttaatggtt gggtatctgt
     7141 acttaacttt tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg
     7201 ccacaccagt ggagtcgtga atgtgttttg tttcaattct gccagcatac cttgcatact
     7261 ggatgcaata tcgccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg
     7321 ttcaatatcc agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccag
     7381 taaccaattc agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga
     7441 acgcgcagct gccgcagaaa gtggatgcgt atcttcgagg atccctttcg ccatagacat
     7501 tggcaggaat ggaatctggg cactttcaat aaattcacga agctgttcat cagattgtga
     7561 atacgccgcg cctttgccaa gaataattaa tggtcgttca gcttttgcta aaagcgaaat
     7621 tgcgctagtg actgacttcg ggcatggcaa taatgctggc gacggatttt caactttaac
     7681 aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc
     7741 aagataaaca ccgccaggac gacccgatac tgagacccgg atagcgcgtg ccaatgcaat
     7801 gccaagatcc tgcggctgat taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt
     7861 catttggtcc agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga
     7921 gccgctaatc atgatcatcg gaaaaccatt taccgttgca ttggccaatg cggtcaaacc
     7981 gttgaggaat cctggcgcag acactgtcag gcagatccct ggtttttggg taagaaaacc
     8041 gcttgctgca gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc
     8101 ttccgcctgg gcatggcgcg ccatatccgt cacaggaata cctacaacac cataaatagt
     8161 gtcaatatta ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag
     8221 ttgatctgac atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt
     8281 taatatcaga tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc
     8341 tcctgcaata cagcagcggt atgttcacct aatagcggcg cagctttaat atccggcgta
     8401 aaggcagaga atttcattgg acaaccaact gtcagatatt ttccacgcaa cggttgttcc
     8461 acttcgacaa cactgccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt
     8521 aaaaccggtg cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca
     8581 atagtgacag tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgggca
     8641 tgggctgtac tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg
     8701 ttttcccagt tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag
     8761 cctttacatt tcaggatcca gccaggctga ccgccaccac ccgcattacc accgcgggga
     8821 actgcatcac caaatgtgcc attcggatac tgcgggtatt cttccagata ccccaactta
     8881 tcgagacgct gctggtcgcg taatttcacg cggcaaaggt tcaacacggc atcctgcatt
     8941 gacatggtga ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa
     9001 ccgatcagca aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc
     9061 ggaccgtccc aaaaacctgt agtagatgcc gcgccacccg ctgcctgagc aacgttttca
     9121 taggctttta cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc
     9181 agacgtggat tgatttcttg aatatgctcc caggtgaagc ccatatgatc aatggcccct
     9241 ggatgaaagt tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg
     9301 ccttccgctg ttttggtatt taattcaata gaacgtttgt tactgttaag catggtgaag
     9361 taaagcgcat cgatatcagg aatatctcgc agctggtggc gcgttacgtc accaacgctg
     9421 ggacgttcaa ttttaatgac gtcagcgcca aaccaggcca gcatttgagt acaagatggg
     9481 ccagattgca caccggtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca
     9541 atacctatta gttaattaaa tatgtgttaa tttagcgttt atttaaggca taacgcctct
     9601 ataaataatc tgcaaaatat cgtcgtagct gcactcgata tttttaaatt cctgtatcaa
     9661 gtgctgaaaa tcatttcatc gctacttcca attcctttct gcatggtaag tagtgaggac
     9721 gaaaagaaga atataataaa tattggttcg cggtaatcag agagagccgc gacatttgtg
     9781 atgttattct tacaactgt
//
LOCUS       DADTFG010000170         9726 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.171, whole genome shotgun sequence.
ACCESSION   DADTFG010000170 DADTFG010000000
VERSION     DADTFG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.171"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(167..1168)
                     /locus_tag="J1138_004047"
                     /pseudo
     CDS             complement(167..1168)
                     /locus_tag="J1138_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1209..2201)
                     /locus_tag="J1138_004048"
     CDS             complement(1209..2201)
                     /locus_tag="J1138_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8455779.1"
                     /translation="MLKKVIFLIAMSGCGSAVADRSTVFYTFNDTLNSPNGRLYSTVG
                     VPVPINISGTSNASLSYRECNGFTWSHGLFWSEYFAWMVIPKSIVYNGVVIYLDIQSK
                     GGWSLDAEDNDNYYFVQGYAWDEVNTSGNTCAFSGDNRPLSWTFGGVTMSAYLPADMP
                     LGMHTIPIKFLRGIQRNNFSYIGGRYKIPSSTMKTFTFRSVSNLNFEVIGGCRPSVQS
                     LDIEHGSLSIDNANGHMVHKSLSVYCDIPASINIYLLPNTPGNYSSTGTNVGLGNGWD
                     SIVSLDGVERDKENIRWSSAGSKTITVGSRLYGEAGKIKPGNLSGSMTMVFSIR"
     gene            complement(2246..2746)
                     /locus_tag="J1138_004049"
     CDS             complement(2246..2746)
                     /locus_tag="J1138_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042092232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8455780.1"
                     /translation="MIRLSLFISLLLTSVAVLADVQINIRGNVYIPPCTINNGQNIVV
                     DFGNVNPEHVDNSRGEVTKTISISCKYKSGSPWIKVTGNAMAGQTNVLSTNITSFGIA
                     LYQGKGTATRLILGDGSGNGYRLTTGLDAANSTFTFTSVPFRSGGSTLNGGEFHATAN
                     ISIIYI"
     gene            complement(2822..3343)
                     /gene="papE"
                     /locus_tag="J1138_004050"
     CDS             complement(2822..3343)
                     /gene="papE"
                     /locus_tag="J1138_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapE"
                     /protein_id="HBA8455781.1"
                     /translation="MKKIRGLCLPVMLGAVLMSQHVHAVDNLTFRGKLIIPACTVSNT
                     TVDWQDVEIQTLSQNGNHEKEFTVNMRCPYNLGTMKVTITATNTYNNAILVQNTSNTS
                     SDGLLVYLYNSNAGNIGTAITLGTPFTPGKITGNNADKTISLHAKLGYKGNMQNLIAG
                     PFSATATLVASYS"
     gene            complement(3370..3906)
                     /locus_tag="J1138_004051"
     CDS             complement(3370..3906)
                     /locus_tag="J1138_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA8455782.1"
                     /translation="MIKSTGALLLFAALSAGQAMASDVAFRGNLLDRPCHVSGDSLNK
                     HVVFKTRASRDFWYPPGRSPTESFVIRLENCHATAVGKIVTLTFKGTEEAALPGHLKV
                     TGVNAGRLGIALLDTDGSSLLKPGASHNKGQGEKVTGNSLELPFGAYVVATPEALRTK
                     SVVPGDYEATATFELTYR"
     gene            complement(3916..4497)
                     /locus_tag="J1138_004052"
     CDS             complement(3916..4497)
                     /locus_tag="J1138_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein papJ"
                     /protein_id="HBA8455783.1"
                     /translation="MVVNKTTAVLYLIALSLSGFIHTFLRAEERGIYDDVFTADELHH
                     YRINERGGRTGSLAVSGALLSSPCTLVSNEVPLSLRPENHSASAGAPLMLRLAGCGDG
                     GALQPGKRGVAMTVSGSLVTGPGSGSALLPDRKLSGCDHLVIHDGDTFLLCRPDRRQE
                     EMLAAWRKRATQEGEYSDARSNPAMLRLSIKYE"
     gene            complement(4534..5268)
                     /locus_tag="J1138_004053"
     CDS             complement(4534..5268)
                     /locus_tag="J1138_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA8455784.1"
                     /translation="MWKLSMIRKKILMAAIPLFVISGADAAVSLDRTRAVFDGSEKSM
                     TLDISNDNKQLPYLAQAWIENENQEKIITGPVIATPPVQRLEPGAKSMVRLSTTPDIS
                     KLPQDRESLFYFNLREIPPRSEKANVLQIALQTKIKLFYRPAAIKTRPNEVWQDQLIL
                     NKVSGGYRIENPTPYYVTVIGLGGSEKQAEEGEFETVMLSPRSEQTVKSANYNTPYLS
                     YINDYGGRPVLSFICNGSRCSVKKEK"
     gene            complement(5339..7849)
                     /gene="papC"
                     /locus_tag="J1138_004054"
     CDS             complement(5339..7849)
                     /gene="papC"
                     /locus_tag="J1138_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /protein_id="HBA8455785.1"
                     /translation="MKDRIPFAVNNITCVILLSLFCNAASAVEFNTDVLDAADKKNID
                     FTRFSEAGYVLPGQYLLDVIVNGQSISPASLQISFVEPALSGDKAEKKLPQACLTSDM
                     VRLMGLTAESLDKVVYWHDGQCADFHGLPGVDIRPDTGAGVLRINMPQAWLEYSDATW
                     LPPSRWDDGIPGLMLDYNLNGTVSRNYQGGDSHQFSYNGTVGGNLGPWRLRADYQGSQ
                     EQSRYNGEKTTNRNFTWSRFYLFRAIPRWRANLTLGENNINSDIFRSWSYTGASLESD
                     DRMLPPRLRGYAPQITGIAETNARVVVSQQGRVLYDSMVPAGPFSIQDLDSSVRGRLD
                     VEVIEQNGRKKTFQVDTASVPYLTRPGQVRYKLVSGRSRGYGHETEGPVFATGEASWG
                     LSNQWSLYGGAVLAGDYNALAAGAGWDLGVPGTLSADITQSVARIEGERTFQGKSWRL
                     SYSKRFDNADADITFAGYRFSERNYMTMEQYLNARYRNDYSSREKEMYTVTLNKNVAD
                     WSTSFNLQYSRQTYWDIRKTDYYTVSVNRYFNVFGLQGVAVGLSASRSKYLGRDNDSA
                     YLRISVPLGTGTASYSGSMSNDRYVNMAGYTDTFNDGLDSYSLNAGLNSGGGLTSQHQ
                     INAYYSHRSPLANLSANIASLQKGYTSFGVSASGGATITGKGAALHAGGMSGGTRLLV
                     DTDGVGGVPVDGGQVVTNRWGTGVVTDISSYYRNTTSVDLKRLPDDVEATRSVVESAL
                     TEGAIGYRKFSVLKGKRLFAILRLADGSQPPFGASVTSEKGRELGMVADEGLAWLSGV
                     TPGETLSVNWDGKIQCQVNVPETAISDQQLLLPCTPQK"
     gene            complement(7907..8494)
                     /gene="papH"
                     /locus_tag="J1138_004055"
     CDS             complement(7907..8494)
                     /gene="papH"
                     /locus_tag="J1138_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapH"
                     /protein_id="HBA8455786.1"
                     /translation="MRLRFSVPLFFVCCAFFRGVFAGPFPPPGMTLPEYWGEENVWWD
                     GRATFHGEVVRPACTLAMEDAWQIIDMGETPVRDLQNGFSGPERKFSLRLRNCEFNSQ
                     GGNLFSDSRIRVTFDGVRGETPDKFNLSGQAKGINLQIADARGNIARAGKVMPAIPLT
                     GNEEALDYTLRIVRNGKKLEAGNYFAVLGFRVDYE"
     gene            complement(8564..9118)
                     /gene="papA"
                     /locus_tag="J1138_004056"
     CDS             complement(8564..9118)
                     /gene="papA"
                     /locus_tag="J1138_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
                     /protein_id="HBA8455787.1"
                     /translation="MIKSVIAGAVAMAVVSFGVNAAPTIPQGQGKVTFNGTVVDAPCS
                     ISQKSADQSIDFGQLSKSFLEAGGVSKPMDLDIELVNCDITAFKGGNGAKKGTVKLAF
                     TGPIVNGHSDELDTNGGTGTAIVVQGAGKNVVFDGSEGDANTLKDGENVLHYTAVVKK
                     SSAVGAAVTEGAFSAVANFNLTYQ"
     gene            complement(9326..9640)
                     /locus_tag="J1138_004057"
     CDS             complement(9326..9640)
                     /locus_tag="J1138_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon regulatory protein
                     PapB"
                     /protein_id="HBA8455788.1"
                     /translation="MAHHEVISRSGNAFLLNIRESVLLPGSMSEMHFFLLIGISSIHS
                     DRVILAMKDYLVGGHSRKEVCEKYQMNNGYFSTTLGRLIRLNALAARLAPYYTDESSA
                     FD"
BASE COUNT         2581 a         2598 c         1996 g         2551 t
ORIGIN      
        1 cagtcatccg gtaaacatcg actttccact acaatttatt cctacctgat ttctgtatga
       61 cagagtagtc ccgacaggca gacggataaa ttcccgcaga taaaagaaca taaatgctgg
      121 ctgctgacaa gtctgtgaaa aaggattatt gtagtggttc acaaaatcag gacaccggaa
      181 taacctgttt ccacttttct tcatgtaagc aaggcggtaa accatcgttg ttcgtgtgag
      241 gtcgataaac gttgtaataa ccattaatcc actggtttat atcacgtacc gcatggataa
      301 aatcaccata accacctttc ggaagccatt catttttaag gctgcgaaag actctttcca
      361 tcggcgaatt atccaggcca ttccctctgc aactcatact ttgcattacc ccataacgcc
      421 agagtaactt tctgtattta ttgcttttat actgaacacc ttgatctgaa tgaaacagca
      481 gacggccatc acgtgatcgt gtttccagtg cattacgcaa agccctacac accaactcag
      541 catcagcggt taatgagagg gctgaaccga taatccgccg tgaatataaa tcaacaacga
      601 gcgcgagcta acaccatttg tcatgcaggc gaataaaact gatgtcgccg caccagacgc
      661 ggtctggtgc ggcggggtga aattgccggt tcagtaaatt tggcaatggc ggacttttgt
      721 cttcgttgac ccggtagtga tgtttaacca tctgtcgact tgtcagcccg cattcccgca
      781 tcagtcgtcg tgccagccac cggcctgcat caacgctact ctggcgcaac atctgactga
      841 ttgcccggct accggctgcg ccacgactga gagcatggaa agccctcacc cgacttcgta
      901 agtcaattct ttgcacatta acaggacgcg tcacctgcgc gtaataaacg ctacggttaa
      961 taccgaataa atgacaaata acccacactg gccactttgc tttcagccgt gtgattagcg
     1021 cgacagcttc ccggggattt cgctcatcag cacggcagcc tgctttagta tttctttttc
     1081 catctcaacg cgctttaagc tgctgaattt cgcgttgttc agggataata gcattaccag
     1141 cggactcaat accctgaaat tcctgcctct ttgtggcgca gatataacaa taaaaccgtc
     1201 ttctttgact aacgaatact aaaaaccatt gtcatagaac ctgacaggtt tccaggtttt
     1261 atctttccgg cctcaccata taaacgactc cccactgtaa ttgtttttga accagcactg
     1321 ctccatctga tgttttcttt gtctcgctcc acgccatcaa gagaaactat tgaatcccat
     1381 ccatttccga ggccgacatt tgtacctgta gaactgtaat tcccgggtgt atttggcaat
     1441 aaataaatat taatgctggc aggaatatca cagtaaacag agagagactt atgtaccata
     1501 tgaccattag cattatcaat tgagagacta ccatgctcga tatctaaaga ctgtaccgag
     1561 ggacgacacc caccaataac ttcaaaattt aaatttgaca cacttctgaa tgtaaaagtt
     1621 ttcatcgtgg atgatggtat cttataacgt ccaccaatat aagagaagtt atttcgttgt
     1681 ataccacgta gaaatttaat ggggatcgta tgcattccta atggcatatc agcaggaaga
     1741 tatgcgctca tagttacacc accaaatgtc catgataaag gcctgttgtc gccagaaaat
     1801 gcacatgtat taccagaagt gttcacctca tcccatgcat aaccctgcac aaagtaatag
     1861 ttatcattat cctccgcatc caatgaccag cctcctttgc tctgtatgtc cagataaatc
     1921 actaccccgt tatatacaat actcttgggt ataaccatcc atgcgaaata ttctgaccag
     1981 aacagcccat gtgaccatgt aaaaccatta cactctctgt aactcaggct tgcatttgac
     2041 gtcccgctta tattaattgg aactggtaca ccgactgtcg aataaagccg cccattggga
     2101 ctattcaatg tatcattaaa tgtataaaat actgtgcttc tgtctgcaac agcagaacca
     2161 caaccggaca tggcaatcag aaaaatcacc ttcttcagca tataaaagac tctctctgaa
     2221 aataagcaac atatacacaa agaatttata tgtatataat gcttatattg gctgtagcat
     2281 gaaattctcc cccattcagg gtagagcctc cactcctgaa aggcacagaa gtaaaggtga
     2341 acgttgaatt cgctgcatcc agaccagttg tcaaccggta accgtttcct gaaccgtcac
     2401 ctaatatgag tcgggttgct gttccttttc cctggtacag cgctatacca aaactggtta
     2461 tatttgtaga cagtacatta gtctgcccag ccattgcatt acctgtgacc tttatccagg
     2521 gggaaccact cttatactta caggatatgc ttatggtttt tgtgacttca ccacgtgagt
     2581 tgtccacgtg ctcaggatta acattcccaa aatcaacaac aatattctgc ccgttattaa
     2641 tggtgcatgg ggggatataa acatttcccc tgatgttaat ctgcacatca gccagtacag
     2701 cgaccgatgt cagaagcaac gatataaata atgataaacg aatcattccc ccctccggag
     2761 agcggtacag aaaacatttt attttacgag atataaaatt aacgtatttt agttgatact
     2821 attacgaata tgatgcaacc agcgttgctg ttgcagagaa aggaccggct atcaaattct
     2881 gcatattccc tttatatcca agtttggcat gaagtgatat agttttatct gcattattac
     2941 ctgtgatttt tccgggcgta aatggagtcc ctaaagttat cgcagtccca atatttcctg
     3001 cattactgtt ataaagataa acgagtaacc catcagaaga tgtgtttgat gtattctgaa
     3061 ctaaaatagc attgttataa gtgtttgttg ccgttatcgt aaccttcatt gttcccagat
     3121 tataaggaca ccgcatattc acagtaaact ctttttcgtg atttccattt tgactcaggg
     3181 tctgaatctc tacatcctgc cagtcaacag ttgtgttgct tacagtacag gcaggaataa
     3241 tcagttttcc tctgaaggtc agattatcaa ctgcatgtac atgctgagac attaacactg
     3301 cccccagcat taccggaaga cacaaacctc ttatcttttt catctgaaat atcctgtaca
     3361 aaaattttgc taacgatatg tcaattcaaa cgtggctgtt gcttcataat caccgggtac
     3421 cacactcttc gtccgcaggg cttccggcgt tgccacaaca tacgcgccga aaggaagctc
     3481 aagactgttt ccggtaacct tttccccctg gcctttgtta tgggaagcgc cgggtttcag
     3541 cagactgctg ccatcggtgt ccagcagtgc aatgcctaac cggccggcat tcactccggt
     3601 taccttcaga tggcccggga gggccgcctc ttccgtcccc ttaaaggtca gggtcacaat
     3661 tttgccaact gctgttgcat ggcagttttc cagcctgatg acaaacgact ctgtcggcga
     3721 acgtccgggc ggataccaga aatccctgga cgcgcgggtt ttgaagacga catgtttatt
     3781 cagactgtca ccggacacat ggcagggtct gtcaagcaga ttacccctga atgccacatc
     3841 tgaggccatt gcctgcccgg cagacagtgc tgcaaacaat aaaagagctc ctgtgctttt
     3901 tatcatcaca ttcccttact catattttat gctcagacgc agcatggccg gattgctcct
     3961 ggcatcagaa tactcaccct cctgtgtcgc ccttttcctc caggcggcca gcatctcctc
     4021 ctgccgccgg tcaggccggc acagtaaaaa ggtatcacca tcgtgtataa caagatggtc
     4081 acagccggat agcttacggt caggaagtaa agcacttccg cttccgggac cggttaccag
     4141 tgagccggag actgtcatcg caacgccccg ttttccgggc tgaagtgcac caccgtcccc
     4201 acatcctgcc agcctcagca tcagaggtgc tccggctgac gcagagtgat tctccggccg
     4261 gaggcttaac ggcacctcat tactcaccag cgtgcagggt gaggacagca gtgcaccact
     4321 gactgccagg cttccggtgc gtcccccccg ttcatttatc cggtaatgat gcaactcatc
     4381 tgcagtaaag acgtcatcgt atatgccccg ctcttcagcc cgcaggaaag tatggatgaa
     4441 accactcagc gacagtgcaa taagatacag tactgctgtt gttttattca caaccataat
     4501 atcccacccg catttaaccg ttattgcggt acattatttc tcttttttca cagagcaacg
     4561 gctgccatta cagataaacg acagtaccgg gcgaccacca tagtcgttaa tataagacag
     4621 ataaggggta ttataatttg ccgattttac tgtctgctct gaacggggag acagcatcac
     4681 ggtttcaaac tcaccttcct ctgcctgctt ttcacttcct cccagaccaa taacagtgac
     4741 ataatagggc gttgggtttt caatacgata cccaccgctg actttgttca gaattaactg
     4801 gtcctgccat acttcatttg gtctggtttt aattgctgcc gggcgataaa aaagctttat
     4861 tttggtctgt aaggctatct gcagtacatt ggccttttca ctcctcggcg gtatttcccg
     4921 gagattaaaa taaaacagtg attccctgtc ctgaggaagt ttactgatat ccggtgtggt
     4981 actcagcctg accatgcttt tcgcacccgg ctcaaggcgc tgaaccggag gggtggcaat
     5041 aaccggccct gtaataattt tttcctgatt ttcattttct atccatgcct gagcaagata
     5101 gggcagttgt ttgttatcat tggagatatc aagcgtcatt gacttctcac tcccgtcaaa
     5161 caccgcgcgg gttctgtcca gcgaaacagc agcgtctgcc ccggatataa caaacagggg
     5221 gatggcagcc atcagaatct tttttcgaat catacttaat ttccacattc tgtaatttca
     5281 cctggtccgg aaaatggcat aaccgcattc atcagccgtt aatattccgg actttcattt
     5341 atttctgagg cgtacaggga agcaataact gctggtcaga tattgctgtc tccggtacat
     5401 ttacctgaca ctgtattttt ccatcccagt ttaccgacag ggtttccccc ggcgtcacgc
     5461 cactcaacca ggcaaggcct tcgtcggcca ccatgcccag ctcccggcct ttttcactgg
     5521 ttacactggc accaaacggg ggctgagagc catcagcaag acgcagtatt gcaaacagac
     5581 gtttcccttt aagcacgctg aatttccggt aaccaatggc accttctgtc agcgccgatt
     5641 ccacaacaga acgggttgct tccacatcat ccggtaagcg cttcaggtca acagaggttg
     5701 tattccggta ataactgctg atgtcagtca ccacgcccgt tccccagcga tttgtcacca
     5761 cctgcccgcc atcaaccggt acacctccca caccatccgt gtcaacaaga agacgtgttc
     5821 caccggacat tccccctgca tgtaacgccg caccttttcc ggtaattgtt gccccaccgg
     5881 aagcactgac gccgaaagac gtatatcctt tctgcaggga tgcaatattc gcggacaaat
     5941 ttgccagcgg actacgatga ctgtaatagg cattaatctg atgttgcgat gtcagtccac
     6001 cgccactgtt aaggccggcg ttcaggctgt agctgtccag tccgtcatta aacgtgtcag
     6061 tgtagccggc catattcaca taacggtcat tgctcatact gccactgtag ctcgctgtcc
     6121 ccgttcccag cggcacggat atacgcaggt aagcagaatc gttatcacgc cccagatatt
     6181 tagaccttga ggctgacaat ccaaccgcca caccctgcag tccgaaaaca ttaaagtagc
     6241 ggttgacgct caccgtataa tagtccgttt tccgtatgtc ccagtatgtc tgacggctgt
     6301 actgcaggtt aaaagaggtg ctccagtccg ccacgttttt attcagcgta acggtataca
     6361 tctctttttc ccgactgctg taatcattac ggtagcgggc gttcaggtac tgctccatgg
     6421 tcatatagtt tcgctctgag aaacgatacc cggcgaacgt aatgtcggca tccgcattat
     6481 caaaccgttt ggagtagctc agacgccagg attttccctg aaacgttctc tctccctcaa
     6541 tacgggctac tgactgcgtg atatcagcgg aaagggtccc cggcacaccc aggtcccagc
     6601 cggcaccggc tgccagtgca ttataatcac cggcaagcac agccccgcca tacagcgacc
     6661 actggttact gagcccccag gatgcctctc cggtcgcaaa tacaggccct tcggtctcat
     6721 gcccgtatcc gcgggaacga ccggagacaa gtttataccg gacctgtccc ggacgcgtca
     6781 gataaggaac cgaggccgta tcgacctgaa aggttttctt ccgtccgttc tgttcaataa
     6841 cctcaacatc aagacgtccg cgaactgaac tgtccaggtc ctgaatactg aatggccctg
     6901 cggggaccat cgagtcgtac agcacccgtc cctgctgcga caccacaaca cgggcattag
     6961 tctccgcaat cccggtaatc tgcggtgcat agcctcgcag tctgggcggc agcatccggt
     7021 catcgctttc caggctggct cccgtataac tccatgaccg gaatatatct gagttgatat
     7081 tattctcgcc cagcgtcagg tttgcccgcc atcgtggaat ggcacggaac agataaaagc
     7141 gactccatgt gaaatttctg tttgtcgttt tttccccgtt gtagcggctc tgctcctggc
     7201 ttccctgata gtcagcccgc aggcgccagg gccccagatt cccccccaca gtcccgttat
     7261 aactgaactg atgagagtct cctccctgat aattacggga aaccgtcccg ttgaggttat
     7321 aatccagcat cagtccggga atgccgtcgt cccagcgtga gggaggcagc caggtggcat
     7381 cagaatactc aagccaggcc tgcggcatat tgatgcgtaa tacgcccgct ccggtatcag
     7441 gacgaatatc cactcccggc aacccatgaa aatccgcaca ctgaccatca tgccagtaaa
     7501 caactttatc cagagattct gctgttaacc ccatcagtct gaccatatct gatgtcagac
     7561 aggcctgcgg caattttttt tctgccttat ctcctgacaa cgcaggttca acaaatgaaa
     7621 tctgtaacga tgcgggagaa atactttgcc cgttaacaat cacatccaga agatattgcc
     7681 ccggcagaac atagccggct tctgaaaaac gggtgaagtc aatatttttc ttgtccgctg
     7741 cgtcaagtac atctgtatta aactcaacgg cactggctgc gttacaaaac agagacaaca
     7801 atatcacaca ggtaatattg ttgactgcaa aaggtattct gtctttcatt ccacgcatca
     7861 ccagattcac aaaaagataa ataaccggac atctcaccgg agtgactcac tcataatcga
     7921 cccggaatcc cagcacggca aaataatttc cggcttcaag tttttttccg tttctcacaa
     7981 ttctgagggt gtaatccagc gcttcttcat tacccgtcaa tggtattgca ggcattactt
     8041 tccctgcccg ggcaatattt cccctggcat cagctatctg cagattaatg ccttttgcct
     8101 gaccggataa attaaactta tccggcgttt caccccggac accatcgaaa gtcaccctta
     8161 tccgggaatc agagaaaagg tttccaccct gactgttaaa ttcacagttc ctgagccgta
     8221 ggctgaattt tctttcaggt ccggaaaaac cattctgtaa atcccgtacc ggggtttccc
     8281 ccatatcgat aatctgccag gcgtcttcca ttgccagggt acaggcaggt ctgacaacct
     8341 caccatgaaa agtagccctg ccatcccacc atacgttttc ttctccccag tactcaggaa
     8401 gggtcatgcc gggcggagga aacggaccgg caaaaacacc acgaaaaaat gcacagcaaa
     8461 caaagaaaag cggaacagag aatcgcagtc tcatgacaaa taccctgaga aataaacagc
     8521 ggaagtgctc cgctgtttac cggacctgat tatcgtatta ctgttactga taggtcaggt
     8581 tgaaattcgc aacggcagag aaggcacctt cagtaacagc ggcaccaacg gctgacgact
     8641 tcttaacaac agcagtataa tgcagcacgt tttcaccatc tttcagggta ttagcatcac
     8701 cttcggagcc atcgaagaca acgtttttac ctgccccctg aactacgata gctgtgcccg
     8761 taccaccatt tgtatctagc tcatcagaat gtccattaac tatcgggcca gtaaaagcca
     8821 gcttaacagt cccttttttg gcgccattac cacctttaaa ggcagtaata tcacaattaa
     8881 ccaattcaat atctaagtcc attggtttgg atacacctcc tgcctcaagg aagctttttg
     8941 aaagctgtcc aaaatcaata gactgatcag ctgatttctg agaaatgctg catggagcat
     9001 caacaacagt tccgttaaaa gttactttac cctgcccctg tggaatagtt ggagcagcat
     9061 ttacaccaaa agacaccact gccatagcta ccgcaccggc aataaccgac ttaatcataa
     9121 ataacaacct ctttttcatt actgaacaca ccagagatat ctggcaattt aaaatgaccc
     9181 gattaccttt aataaccaga tcaatacaaa taatattctc gtattttcag ggcaggttat
     9241 ttatcgacag aatactgata taacctgtct gctttctgat aagggcttct ttttttatct
     9301 tccagaaact ccggaatgcc ataatttagt caaatgccga cgactcatct gtataataag
     9361 gtgcaagcct tgctgcaaga gcattcagcc gtataagtct ccccagcgtt gtactgaaat
     9421 acccattatt catctggtat ttctcgcaga cctccttacg ggagtgccca cctaccagat
     9481 agtccttcat agccagaatg accctgtcac tgtgaataga agaaatacct atcagtaaaa
     9541 aaaaatgcat ttcagacata gagccgggca acagtacgct ctcgcgtata ttcagcaaaa
     9601 acgcatttcc tgaccgactg atgacttcat gatgcgccat gtttccccct tctgtcgggc
     9661 ccctggatat atgcttccgg cccggaaata ctcttcacgc aataagttaa atttagtttt
     9721 ttatgt
//
LOCUS       DADTFG010000171         9633 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.172, whole genome shotgun sequence.
ACCESSION   DADTFG010000171 DADTFG010000000
VERSION     DADTFG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.172"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            178..1242
                     /gene="ulaG"
                     /locus_tag="J1138_004058"
     CDS             178..1242
                     /gene="ulaG"
                     /locus_tag="J1138_004058"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBA8455789.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GTLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1350..2105
                     /gene="ulaR"
                     /locus_tag="J1138_004059"
     CDS             1350..2105
                     /gene="ulaR"
                     /locus_tag="J1138_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBA8455790.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2102..2851)
                     /gene="yjfP"
                     /locus_tag="J1138_004060"
     CDS             complement(2102..2851)
                     /gene="yjfP"
                     /locus_tag="J1138_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA8455791.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTIKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3033..3362
                     /gene="bsmA"
                     /locus_tag="J1138_004061"
     CDS             3033..3362
                     /gene="bsmA"
                     /locus_tag="J1138_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBA8455792.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3511..3786
                     /gene="yjfN"
                     /locus_tag="J1138_004062"
     CDS             3511..3786
                     /gene="yjfN"
                     /locus_tag="J1138_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBA8455793.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3903..5528)
                     /locus_tag="J1138_004063"
     CDS             complement(3903..5528)
                     /locus_tag="J1138_004063"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBA8455794.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGASWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGVRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHRLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5612..6775)
                     /locus_tag="J1138_004064"
     CDS             complement(5612..6775)
                     /locus_tag="J1138_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBA8455795.1"
                     /translation="MLRHNVTVRWDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDTRCSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6778..7416)
                     /locus_tag="J1138_004065"
     CDS             complement(6778..7416)
                     /locus_tag="J1138_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBA8455796.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7426..7824)
                     /locus_tag="J1138_004066"
     CDS             complement(7426..7824)
                     /locus_tag="J1138_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBA8455797.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7842..8501)
                     /locus_tag="J1138_004067"
     CDS             complement(7842..8501)
                     /locus_tag="J1138_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HBA8455798.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDAL
                     LIELPGEEYTVAAVSCIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPMTSLHII
                     G"
     gene            complement(8551..9249)
                     /locus_tag="J1138_004068"
     CDS             complement(8551..9249)
                     /locus_tag="J1138_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBA8455799.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9268..>9633)
                     /locus_tag="J1138_004069"
     CDS             complement(9268..>9633)
                     /locus_tag="J1138_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HBA8455800.1"
                     /translation="QTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQMIIETFI
                     CPVSSISNPDEFNTFLLRNQKLMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEI
                     TSLVDNALDLAEITEEYSH"
BASE COUNT         2521 a         2536 c         2272 g         2304 t
ORIGIN      
        1 ttcatttagt taattgcatt acacattcaa tttaatcttt ccacactcaa aatccacaat
       61 aactctcctg ctaaaaagtc aaaaaatggt cattgccttc ctgaaaaaat agtgtcagga
      121 ttaatcaaaa ccaatcacat attgattcga attgaactat cacgaaggta aatggcgatg
      181 agtaaagtga aaagtatcac ccgtgagtcc tggatcctga gcactttccc ggagtggggt
      241 agctggctga atgaagaaat tgagcaagag caggtcgcac ctggcacttt cgcaatgtgg
      301 tggctcggct gtaccgggat ctggttgaaa tcagaaggcg gtgccaacgt ttgtgttgat
      361 ttttggtgcg gtactggcaa acaaagccac ggtaacccgt taatgaaaca gggtcaccag
      421 atgcagcgca tggctggcgt gaaaaaactg cagccaaacc tgcgcaccac cccgtttgtt
      481 cttgatccgt ttgcgattcg ccagatcgac gcggtactgg cgactcacga tcacaacgat
      541 catatcgacg ttaacgtcgc tgctgccgtg atgcagaact gtgctgatga cgtaccgttc
      601 attggaccga aaacctgcgt tgatttgtgg attggctggg gcgtaccgaa agagcgttgc
      661 atcgtcgtta aaccgggcga cgtagtgaag gtaaaagaca ttgaaattca tgcacttgac
      721 gctttcgacc gcacggcact gatcaccctg cctgccgatc aaaaagcagc tggcgtactg
      781 ccggacggca tggacgatcg cgcggtgaac tatctgttca aaaccccagg cggtaccctg
      841 tatcacagcg gcgattccca ctactctaac tactatgcaa aacatggtaa tgagcatcag
      901 atcgacgtgg ctttaggttc atacggcgaa aacccacgcg gtattaccga caaaatgacc
      961 agtgctgata tcttgcgtat gggcgaagcg ctgaatgcga aagtagtgat cccgttccac
     1021 cacgatatct ggtcaaactt ccaggccgat ccgcaagaga tccgcgtgct gtgggagatg
     1081 aaaaaagatc gcctgaagta tggcttcaag ccgtttatct ggcaggtcgg cggcaaattt
     1141 acctggccgc tggataaaga caacttcgag taccactatc cgcgtggttt cgatgattgc
     1201 ttcactattg aaccagatct gccgttcaag tcgttcctgt aatctgtcgc ttgtaccgga
     1261 tagcgtagag atgctcgcgc tatccggcat agtatttcct ggctatttca aatatcatct
     1321 aaaaaaatca aattttatcg gaatagctca tgactgaagc acaaagacat caaatcctcc
     1381 tggaaatgct cgcacaattg ggctttgtga ccgttgagaa agtcgttgag cgtctgggaa
     1441 tttcgcctgc cactgcacga cgcgatatca ataaacttga cgaaagcggc aaactgaaaa
     1501 aagtgcgcaa tggcgcagaa gctattaccc aacagcgccc gcgctggacg ccgatgaatc
     1561 tgcatcaagc gcagaatcac gatgaaaaag tacgtattgc taaagcggcc tcgcagctgg
     1621 ttaatccggg cgaaagcgta gtcatcaact gcggctccac cgcgtttctg cttgggcggg
     1681 aaatgtgtgg caagccagtg caaatcatca ctaattatct accgctggca aattacctga
     1741 tcgatcagga gcatgacagc gtgatcatta tgggcggaca gtacaacaaa agtcagtcca
     1801 tcactttaag cccgcaaggc agcgaaaaca gtctctatgc cgggcactgg atgtttacca
     1861 gcggcaaagg actgacagca gaagggttgt ataaaaccga tatgctgacg gcaatggcag
     1921 agcagaagat gctgagcgtg gtagggaaac tggtggtact ggttgatagc agtaagattg
     1981 gcgaacgcgc gggaatgctt tttagccgtg ccgatcaaat cgatatgctg atcaccggca
     2041 aaaatgctaa cccggaaatc ctgcaacaac tggaagcgca aggggtcagc attctgcgtg
     2101 tttaaagatg ctggcggaaa aatgtcacgg cagcatctaa cgcctcaggc gtaatgcggt
     2161 gacgcacgcc tggctgccat gaacaggtta gcagtttatc ccgccccgtc tcgcttaagg
     2221 cctgctgcaa acgtagtgat tcgtcggcag gcacgacgtc atcgtcaagg ccatgccaca
     2281 gcagtagagg tctgtcacca agttgttcca ggtggtttgt cgcttcccac tctgccagcg
     2341 gcgcgacaat gttattgaat tcattctgct gtgctgccgt ttcaggtatc agcggtggaa
     2401 acagtgaacg ggcgagcgat gtaaaatagc ccgatcccat catgctggcg gtacatttta
     2461 tcgtggggtg gcgagcggta attcccagtg ccgtcatcgc gcccatcgac gcgccaccga
     2521 ctgccagacg gtcatcaagc agccagtttt cttcggctat tgccgcacgt aaggtagtga
     2581 attcctgcat actttgtagc aagatttgcc agaattgatt taaccgccgc gctgcgtcgc
     2641 cactaaaacg gctaccgtga tcgggcgcat ccggcatgat cacccgcaaa ccagcctgcg
     2701 ccagcgcaac ggcaaaatag ctatacacca gactggacga agtaaagccg tgataaaaaa
     2761 tcacacacgg taacggggta tctttttgcc cgacaggata agcatgaaga acgggaatat
     2821 ctgccagctc gcgtgattct atttcaatca tctgtctctc ctttttgtct ggtatgacat
     2881 gaagaaatcc ttagcacaag tttctgctgg cggtattagt tcataatgtt gagatgtggg
     2941 ttacgctttc gtcacgtttt cattcgaaaa tcgcgtacca ggtaacaatt cgggaacatt
     3001 cccccaaagt taaattaata aggcactaca ctatggttag caggaaacgt aatagcgtta
     3061 tttaccggtt tgccagttta ttattggtgc tgatgttaag tgcctgtagc gcactgcaag
     3121 gtacgccaca gccagcaccg ccagtaacgg accatccgca agagattcgc cgcgaccaga
     3181 cgcaagggtt acagcgaata ggtagcgtaa gcacgatggt tcggggttct ccggatgacg
     3241 cattagcaga aatcagagcg aaagctgtcg ctgcaaaagc tgattattac gttgtcgtca
     3301 tggtggacga aaccatcgtg acaggacagt ggtattcaca agccattttg taccgtaaat
     3361 aaatcagaca acttacatag tcagctttac attgctttgc gtccctatgc gtttacctgt
     3421 gcacaataaa ttaccggctg gaagctcggg cgtttattac cgcgatggaa tgccctgcaa
     3481 catgtgggga aacgaaaaat ggagctgacg atgaaacaat tacttgcctc accctcgctg
     3541 caattagtga cttatcctgc gagcgccacg gcacagtctg cagaattcgc cagtgctgac
     3601 tgcgtgacgg gcctgaatga aataggtcag atctcagtta gcaatatttc aggggatccg
     3661 caggacgtgg aacgtatcgt ggcgttaaag gccgatgaac aaggtgcgtc atggtaccgc
     3721 attattacca tgtacgaaga ccagcagcct gataactggc gcgtacaggc gatactgtat
     3781 gcgtgagtgt catctacagc aaatgaacaa tgccggatgc ggcgcgagcg ccttgtccgg
     3841 cctacaattt taaaatattt catttagtta atcgaaacca gcgtcgcatc agtcgtatac
     3901 gcttacaaac acactcctcc cgtcgcccgc agcaataaat cattctggat ctgctctgac
     3961 aaccgtacgc cgccgcgcgt atctaacatc acctgacacc acgcctgcgc cattggcgga
     4021 gaggcatatt tcaacatttg cgcaccgcag cccagcagga atagccgatg agtaatctct
     4081 cgccccaatt cttcagctgg cttacgcagc tgctgctgta aacgacgaac cgcgcgatca
     4141 aaatagcgat cctgcccttt cacttccaca aatgcttccg acaataagtc gtatacgccc
     4201 gcttgcttat tgagaacgcg cagcacatcc aggcacataa tattgccgga accttcccaa
     4261 atactgttta ccggcatctc ccggtaaagc cgcggcagct cgctctcctc gcaataacca
     4321 atgccgccca gcacctccat cgcttcggcc acaaacggaa tgccacgttt gcagatcaca
     4381 aatttcgccg caggcgtaaa taaacgcgcc cacagagctt ctttggcatc ggcgcgccgg
     4441 tcccacgctc gcgcaagacg aaacagcaac gccgtttgcc cttcaagctg aagtgccatg
     4501 cgacttaaga catgacgcat aaggggctgt tggatcaatg gattaccaaa aacatggcgt
     4561 tgatgtgcat gataaatagc cagcgaaaat gcacggcgca tcatggcatg gctacccagg
     4621 gcgcaatcaa aacgcgtcat cccacccatt ttcaggatca gacgaactcc ttccccttcc
     4681 tgccccaaca accagccaat ggcatcctga aactccactt cgcagctggc gttagagcga
     4741 ttacccagct tatctttcag ccgctcgagg cgaatcgcgt tgcgttgccc gtcaggcaaa
     4801 aagcgcggca caaaaaagca ggacaaaccg cccgcggtct gtgccagtac cagatgcgca
     4861 tcgctttgcg gcaccgagaa aaaccattta tgccccacca gccgataaga accatcgtcc
     4921 agacgctctg cgcgggtggt gttgctcatg acatcggaac cgccctgctt ttccgtcatt
     4981 cccatgccga tcaacaagcc acgtttttgc ccacctggca ataagtgaga atcgtagcga
     5041 tcgctcagca gcggcgtggt ccagtcctga aacggcgcgg gtaacatctg taacaacaat
     5101 ggcgtggcgg caaaggtcat cgttatcgga cataacgacc cagcctcaac ctgcgcatgt
     5161 aacataaaac gcgccgcgcg cgccacaaat gcgccggagc gagcgtcttc ttcccaggca
     5221 agattgtgca cccgattggt acatagcgcc tgcatcagca ggtgccaggc ggggtgaaaa
     5281 cgcacatcgt ccagacggcg tccttgcgca tcgtagcgca ataattcagg cggattcaca
     5341 ttcgccagcc gccccagttc aagggattca gccgttccta actgctgacc aatactggcg
     5401 agaaaatcgc tatcccagct agcaccttca cgcgttaccg cttcgcaaag cgcgccatca
     5461 gacaggtata agttgctgtt atttaatggt ataggttgat taaaaacggt gtgagtttgc
     5521 cagtgcactg tgtctccctc catcaatggc agtcaccatt agtatggtca ctgccatgga
     5581 ttcatgacag aaagagattg ctaaaacatt cttatccagc aatgtaatgc ggaacgaaac
     5641 gtgaggtgtc tttggtgatc agcgtgttat cttcacggat ccccattccg cacgcttcat
     5701 catcgacaat ccaactaccg atgagtgtgt agctatcgcc aaatcgcggc agaggttgaa
     5761 acgcctggta gatcatcggt tcatcggcgt aatcaccatc agcgtggtca acaacgttat
     5821 tctgaccgtc gaaaatggtg acgtttccgc cttcgcgcga gtaaatcggt ttacgcacat
     5881 agctttcgcc agcggcaatc tgcggttttt caccatcgaa ccaggatgca agaaggttag
     5941 gatgaccagg gaagaagcgc caaagcagcg gcattagccc tttattactc aagatacttt
     6001 tccataacgg ctccacccat tgctcacgac gcttgcgcag cagcgggccg ttatcgtcac
     6061 gcatcatcca ctccagcgga tacagcttaa atgcacgctg gatgacatta tcatcaagat
     6121 cggtcagtac gccgccgacg cccaaaccga gctcttcaat gtagataaac cgagactcct
     6181 gccctgcctg ctcggcgcag tcctgcaagt acagcacggt actcctgtct tcgtcggtat
     6241 cctgacagca gcaaaaataa aacggttccc gactgtaaag ctcgctaaag cgtgaaatca
     6301 ggcgctcctg aatggcattg tactgatcgg catcgcgcgg aataatgccg ctgcaccggg
     6361 tatcttccag ccacaaccac tggaaataag ccgactcgta caatgaagtt ggcgtatcgg
     6421 cgttgtactc cagcaactta accggtgcat taccacacca ggcaaaatcc atacggccat
     6481 acaacgaagg atcgcgtgcg cgccagcttt cggcgatcac atcccagtac aacggcggga
     6541 tcgccagttg cgtcaggatc tcttcatctt taaccgcgcg atccaccacc tccaggcaca
     6601 tctgatgcag ttccgcagtc ggtttttcga tctgctcttc aatctggcgc agagtaaaac
     6661 gataagcccg actctcatcc caatagattt cattgtcgat gatatgaaag tcaaatccgt
     6721 tatcggcggc aatccggtcc agatcccacc gcacagtaac gttgtgtctc agcatgatta
     6781 gcctccccaa tgcccacggg cgctggaaga acgaccatag ccgccgcgag aaacggttga
     6841 cgcttttttg gtggtaaagc cgcccgaaga gtaagactct tttttgccag aaccggagcg
     6901 ccaggagtaa tcaccggaag tgttgcgcca gacggggcgg gaagcaaatg aggatccgcc
     6961 gctgttatag acgaacggct catcgcgatc tttacgaata acccggctta acaaaaatcc
     7021 agaaacgacc gggatccaac tctgttcaac attgtcgtag tagcaatttt cgtacttaga
     7081 ctgacaattc tgctgagtca tattcttcgg aacatcggca taaaatgccg ctttggcgtt
     7141 gttccagcca tgagcacaga tatctgaatt attaccatca tcaatacaat cctgcacagt
     7201 cgcgtaaaac gttccgtcgc cgtcgttatc gacatcgcca ctatcgccac aacctttcaa
     7261 tacgaaaaaa gcggcaccgc ccattagtgc caacgttaag tatttttgtg cgtagcgatt
     7321 gcggcgcggc tcaaaaggat tatgcggtct accaatgcga ctgattgccc catgtccgat
     7381 atttttgtta tttctggatt tgcgttttct ggccatgatc gtccattacc atgtcataca
     7441 agctgcgtta aaaataccgc cagccagcgc ggcggttccc ataaacattc ctgctgcggt
     7501 attgtgatta ataatttttt cgcttaatgc aggcatataa agcctgacgc cagcaaaaac
     7561 gagtaattga atcaccagag caatcccgcc ccatgcgaaa tagtctggaa tactcaccgc
     7621 attgattgcc gcactggata aggggatcac gtaccccaac aatgttccgc tgaacgccag
     7681 tgccgccgcg gtgttattgt ttttgattaa ctgccattcg ttgtgcggtg taattttgga
     7741 gtagataaac aggaaaataa tcaccatcgc cacgccaata aaaaaatagg cactaaaggc
     7801 aagaagtgaa tccagtatat gcattgtaaa cgtccttgta attaaccaat aatatgaagt
     7861 gatgtcatcg ggatatcgac acccagcgcg cgcgaaaata accactccgg ctcgccgaga
     7921 tcgttaaaag attcctcacc atttaacagc aaatattcgt aagtatcttc ggtcacctgc
     7981 cgttggtagc ccatgctaaa attatgcact tcccatttag catggttttg attttctact
     8041 ttttccagca tgtagacggg ttcgatattt cccggttctt cgctgttaaa aaatcgctgc
     8101 cagcgttttt cctgccagtt taaggtcatt gcccccatcg ctttggcgtt gatcgcctcg
     8161 cgccagtgac tctctttact gataccgtag ctctcttcat agacaaagag cttgatatca
     8221 tcaatgtcat caatatcttc gccgccagtg gtattgattt gcaggaactc atcgcccgaa
     8281 gtgtagtaac ggaaaatctg gctacccccg cccagatcga tgcaactgac ggcggccacg
     8341 gtatattctt cacccggtag ctcgatcagc aatgcatctt ccagcaaacg aaacgctaac
     8401 gtatcgagcg taaaaccact attgagatgc agacccaacg gaccgcgagc gatagcaggc
     8461 ttattatcct tgccaaacag acgctggaaa aaaccagaca tattttatcc ttaaaatagt
     8521 tgccagcctt gcagtgaggc tggcaaaaaa ttactcgccc tgttggcgtt gcagtcttgc
     8581 taatacatcc tgggcgctac ttttattgct accgccaatt ccggcttccg ccagcttttc
     8641 atcgagatcg cgaccgtctg cgactttttc caactgtgcg gcagcatcca ggcgagcctg
     8701 acgttcggcc tgacgcgttt gcaggcgttt taaggattct gccgctgtcg aaacgctgga
     8761 agatgcgcca acagtagagg tcgttacagc ctgttgtgca cgctgcatgg cttcagtggc
     8821 tttaacgact tccatttgct gctcaaactg agcgatacgc tgcgctgtcg ctgtaactgc
     8881 tttttccacg ccatcacgag agacttccag attcgacaac acttgctctt cagcggtaat
     8941 gagattctca aggcgcgcga tttcttcagc aacttcgtta atcaacgacg gattcacgtt
     9001 cttgcttaac gcttccagcg cacgagcttc cagactggct ttgcgctcac gtaaatcttt
     9061 cagcttatcg tgacttaatt tcactcgcgc cagcagatca acgcgagatt ttccggcttt
     9121 atcgagttca gctttagcgt cacgaatatg ctgttccagc atgcgcacgc cctgggtttc
     9181 ttcaatggac tcttccgcct gggagataaa cgatttcccc agcgtaaata aactttttaa
     9241 aattcccata aatacccgtc cttgtaatta gtgtgaatat tcttctgtaa tttcagccag
     9301 atccaatgcg ttatctacca gcgaggttat ctcaagcagg atatcttcaa gagaagattt
     9361 aagcgataac gcaccgaaaa caatgtaata ctcttcctgt tgcacgctgg agatccctac
     9421 cgatgacaat ggcatcagct tctgatttct taataagaag gtattaaatt catctggatt
     9481 gctgatacta cttaccggac aaataaaggt ttcgatgatc atttggcggg aagtaaaaag
     9541 aatattgatt tgcaaatcgc cataatcatt cattttaata attaatgcgc tttcgctatt
     9601 tgttacatca atattttgtt caggtacagt ttg
//
LOCUS       DADTFG010000172         9613 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.173, whole genome shotgun sequence.
ACCESSION   DADTFG010000172 DADTFG010000000
VERSION     DADTFG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.173"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            4..144
                     /locus_tag="J1138_004070"
     CDS             4..144
                     /locus_tag="J1138_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HBA8455801.1"
                     /translation="MIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFS
                     LF"
     gene            complement(263..1780)
                     /gene="lysS"
                     /locus_tag="J1138_004071"
     CDS             complement(263..1780)
                     /gene="lysS"
                     /locus_tag="J1138_004071"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBA8455802.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(2017..3474)
                     /gene="dtpC"
                     /locus_tag="J1138_004072"
     CDS             complement(2017..3474)
                     /gene="dtpC"
                     /locus_tag="J1138_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBA8455803.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(3533..5680)
                     /gene="cadA"
                     /locus_tag="J1138_004073"
     CDS             complement(3533..5680)
                     /gene="cadA"
                     /locus_tag="J1138_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBA8455804.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(5760..7094)
                     /gene="cadB"
                     /locus_tag="J1138_004074"
     CDS             complement(5760..7094)
                     /gene="cadB"
                     /locus_tag="J1138_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBA8455805.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDNNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7460..8998)
                     /gene="cadC"
                     /locus_tag="J1138_004075"
     CDS             complement(7460..8998)
                     /gene="cadC"
                     /locus_tag="J1138_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator CadC"
                     /protein_id="HBA8455806.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         2702 a         2236 c         2181 g         2494 t
ORIGIN      
        1 tatgtgattg gtgtgaacat atcctttgtc attatctggt ttatctcaca tgagaaaaca
       61 catattcgtt tacttagtgc atttctggtc ggaataacct ggccaatgag tctgcctgtg
      121 gcattacttt tttctctctt ttaggagctg tagttgagtg gacccgctgg ttatcttgaa
      181 acgaagcgtc tctcctcaaa actggatttg cggaatgacg aacgaagcag tcgcctgact
      241 gcttcgttca ttaaagtgaa atttatttca gtgggcgcat cgccgggaag agaataacgt
      301 cgcgaatagt atgactgttt gtaaacagca taatcattcg gtcgatacca atacccagac
      361 cagcggttgg cggcagacca tattccagcg cagtcacgta atcttcgtca tagaacatgg
      421 cttcgtcgtc acctgcagct ttagcgttaa cctgttcctg gaaacgttca gcctgatctt
      481 ctgcgtcgtt taattcgcta aaaccattac caatttcacg accaccgatg aagaattcaa
      541 aacggtcggt gatttccggg ttaacatcat tacggcgcgc cagcggggac acttctgccg
      601 gatattccgt aataaaggtt ggctgaatca gatgtgcttc tgccacttca tcaaagatct
      661 ccgtaacaat acgtcccagt ccccagcttt tctctaccgt aataccgata gattcagcta
      721 atgctttagc agcatcaaaa ttatccagat ccgccatatc ggtttctgga cgatattttt
      781 tgattgcttc gcgcatggtg agtttttcaa acggtttgcc gaaatcaaac acatgctcgc
      841 cataagtgac tttagtggta cccagtacct cttgtgccag ggtgcggaac agtgactccg
      901 tcagttcaat caaatcatgg taatccgcat atgccatgta gagttccatc attgtgaact
      961 caggattatg gcgaacagaa ataccttcat tacggaagtt acggttgatt tcgaataccc
     1021 gttcaaaacc gcctacaacc agacgtttca gatacagctc cggcgcgata cgcaggtaca
     1081 tatctaaatc cagagcgtta tgatgggtaa taaacgggcg agcagatgcc ccacctggaa
     1141 ttacctgcat catcggggtt tctacttcca taaagccgcg tgcgaccatg aactgacgga
     1201 tagcggccag aatttttgaa cggacaacaa acgtttgacg ggatttatcg ttagcgatga
     1261 ggtccagata acgttgacga taacggactt cctgatcctg cagaccatgg aatttatctg
     1321 gtaaaggacg tagtgcttta gtcagcaggc gcagctcagt acagtgaatg gaaagctcac
     1381 ccgtttgcgt cttaaacagc gtaccgcggg caccgataat gtcacccaga tcccattttt
     1441 taaactgatc gttataaaca ccttctggca ggctatctct tgcaacgtac agttgaatac
     1501 ggccaccgac atcctgcaac gttacaaagg aggctttccc cataatacga cgggtcatca
     1561 ttcggccagc aaccgagact tcaatgttta aggattccag ttcctggtta tccttcgcat
     1621 caaactcttc gtgcaactgg tcagaggtat ggtcgcggcg aaaatcattg ggaaacgcca
     1681 caccttgctg acgtagtgcc gccagttttt cgcggcgatt tctcagttca tcgttaaaat
     1741 caatagcctc attggctccc cgtgtttctc gttcagacat tttggttcct ctaaatccag
     1801 ctttcaattt agcgtagata aagagacaga tcggtacact gtattatctg cctcgactat
     1861 taataactca gtttatctta tcgaaactaa cgtttacctc aagttgatat cactaataaa
     1921 taaccataaa aatggtttaa atgacttatt aatctcgatt tgctagtgtg ataaactgaa
     1981 agttaattta ttcataacca attgttttta cgacagttaa tcgttgctct cctgtatcat
     2041 attcgtcggc gtgctaaaca gaaaacgggt agcaaaggcc aataccacga tgatcgcgac
     2101 acaagccaac gtccactctc ccatctgaga aaagaatcgc tggtaagcgg caattgccat
     2161 accgctaatt tgcgactctg tcgtctgctg tgccacgacg cctgccagcc agttggcgac
     2221 tgcgcctgtt gccagcatat aaataccagt taatacgcca gacattttca gacgcgtgat
     2281 ttgcgcaatc gccaccgggt caataaagag ttcagcaaag cccattagcg ccagcccgga
     2341 tatcatcacg cccattgacg cttgaccgcc agccgctgca tgtcgggcat caaatgccag
     2401 caacataaag ccacaagcca tcagcagtaa gccaaaggca aacttcagcc agacgcgcaa
     2461 tgctgaattg ccgcggcttt ctggcgacgc cagccaggcc agaacaaccc cagcgagcat
     2521 caccgcaatg gcattcaccg actggaatag tgctgtaggt acttcaatat tgaatgcctg
     2581 acgattcaca aagcgatcga taaacaagct gatggtactg ccgccctgtt gtgccagtac
     2641 ccagaacaac gtcccgacaa acatcaacaa tacaatttgc caaagagcac ggcggtgttc
     2701 ggggaatttg atcatcatgc gggcaatgat ttgtgcggca atgaggcaaa cgatcgccag
     2761 caaatatccg gaccagtcgt tctccagcag cagggtaaaa aatactgggg ctaaacagag
     2821 catcaccact aaccagctcc atactggtaa ggcaaatttg acgctagtga gcgctttttt
     2881 atccatacta cgcgtggact ggaaatgacg atgaccactt aagaaaatca acaaaccgat
     2941 aaacatgccg cctcccgcaa gggcaaagcc aacatgccat ccataccact gagcagccag
     3001 gccgcaggcg atgggcgcgg cgatagaacc gatattgccc gcagcataca gcagcgaaaa
     3061 accgccatca cgtcgatgat cgttctcgtc gtagagctcg ccaagcaaac agctgatgtt
     3121 tgatttgaat aaaccgtagc cacaaataat gattgccagc gccagataca ggctaaaggt
     3181 tgaatttgta tcaatgccca gcaccacatg gccaagggtc attaacagcg cgccggcaat
     3241 cactgcagtg cggttgccga gcaggcggtc ggcaagccag ccgccgagaa taggggtaac
     3301 gtaaaccaga gaagcatatg cgctgaacag gctgatggca tggttatcat caaaaccaag
     3361 ctgatgggtg agatagagaa tgagtaaggc acgcatgccg taaaaactga agtactccca
     3421 gatttggatc gccacgatat agtatatcgc gcgcggctgt gagggtgttt tcatgtgttc
     3481 tccttatgag caaaaaaggg aagtggcaag ccacttccct tgtacgagct aattattttt
     3541 tgctttcttc tttcaatacc ttaacggtat agcggccatc agcctgacgg tatgcaccgt
     3601 gaatatcggt ttcaaagccc ggatagtgag cgccgatttc acacagcatc tgcaggaact
     3661 ccagaaccgg acggctttct tcggtgatca tttcacccgg cattaccaga ggaactcccg
     3721 gcggatacgg aaggatcata ttggcgttaa tacgacctac catttcgtcg aggtaaactt
     3781 cttcggtcat accgtgcagc tctttctgga acgcagcata cggagtcatt accatagtcg
     3841 gcagaacttc aaatgcgcga tacatcagat ccggcagatt gtggtgaaca atcagtttgt
     3901 ggatattctg agccagttcc tgaatacgca tgttttcata gaattcagga tcttcacgat
     3961 acagagacgg cagcatgttt ttcacacgca ggttcagatc gaacgcacgt ttgaagtcag
     4021 tcagagcacg cagcaggctc agtgctttgg tcttatcgat accgatgctg aacaggaaca
     4081 gcaggttata cggaccggtt ttctcaacaa cgatgccatg ttcgtcgagg tatttcgcca
     4141 cgatgctggc cggaatacca aagtcgctca tggtgccgtc tttttccatc cccggagtca
     4201 gcagggtgac tttgatcggg tcaagataca tgtgctcgtt atcgatgttt ttgaagccgt
     4261 gccaggtgct gtcagaacgc agcggccagc attcagtcgt atcgatatga tccggctgcc
     4321 atacatcaaa gaaccagcca tcagattccg ttctcagacg tttgatctct ttacggaatt
     4381 tgatcgcacg ttcaatggaa ccattgatca gacgtttacc tgcattgcct ttcatcatcg
     4441 ccgcagcggt ttcagtggac gccacgatac cgtagtgcgg agaggtggtg gtgtgcatca
     4501 tgtaggcttc gttaaaggtt tcttcgttta cgtcaccttt aacgtggatc atggaagcct
     4561 gagagaacgc cgccagcagt ttgtgagtag actgggtttc gtaaatcact ttcccttcta
     4621 cacggccacc gctcataccg catttacctt cgtaaatcgg tgagaagttg gtgtaaggca
     4681 cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg aagtcggtgt
     4741 tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc caggttgcgt
     4801 ttggtgtttc tttcacgcgc ttagcaatgg tagcgtgctg gaattcactc tgtgggatac
     4861 caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta acatcgctca
     4921 tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg gtgctgcctg
     4981 ccggagcaga gtacatacca acaattttgt tcgcagtgga agtaccgttg gtcaccatgt
     5041 agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg tgtggaccac
     5101 tgtgatccag cagagaaccc aattcagata ctgaaatgga aatatcagat ttcatggtgt
     5161 tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat gcagtaccac
     5221 ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta aacagtgctt
     5281 tggtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg atcttgttag
     5341 caatatcatc agcagcaccc agcgcatatt caaagaagct aatctgtaaa cgcaggtcat
     5401 tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc aggttctcgt
     5461 tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca aaaataacgc
     5521 cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg ttcgggtaaa
     5581 caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt tcttctttaa
     5641 aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct ccaggtaaaa
     5701 aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa tacttttaat
     5761 taatgtgcgt tagacgctgt gtggttatcc attgagtggc tctggcgctc gtgcattttg
     5821 cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag ctcgaaggag
     5881 cttgcgccca tcagcgcgat gaagcagaac acgcaaccca gtacagagca gatcaggctg
     5941 acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga gtagaagtac
     6001 ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc agatgcttta
     6061 ccaccggcag agttcatcag agtgatgagg atcatcaggg cagtcatttt cactgcagcc
     6121 agcagcagac cttttttcgg aataccgttg ttgtcgactt caccataaac tttcgggaag
     6181 ttaccgtcgt tagcggcacg tacacctgcc tggcctacca acatcatcca ggagcccaga
     6241 gaagtcagac acgcaaaggc ggtgaatgca gaaaccagtg gcgcagccca gttaccgagg
     6301 atagttgaag cactgattgc aaacggagca ccggaagccg ccattacaga agacggatac
     6361 ataccggaaa gcacctgagt cgcagcgatg taaacaatac ctgctaaacc agtacccagc
     6421 atggttgcca gcggaacggt acgtttcggg tttttaacca taccagtact tactgctgcg
     6481 gattcaactc ccacgaaggc ccacaggcag agcagaatac ttttaatgat cgcatgacca
     6541 tcagtggtat ccgcagtatt ccagttagct gcataagttg ctgcatcaaa ccaatgccag
     6601 ccaacaatag cagtcatcac cacaggaata agaaccagca ccagaccaat agtggttaaa
     6661 cggcttaccc aggtaccgcc gagcatattt acaaaggtaa atacccagac gatagcaata
     6721 caggcgatac ccgccggaac aggatcattt aatactggga agaaggtgga aagataagat
     6781 acagcggtaa taccaattgc caggttacca atccagttag catggtaata aagaacacct
     6841 gtctgaaaac caaatgcagg ggaaatttct ccggcatacg caattgggcc accttgttgc
     6901 gggttttttg ttgccagtcg ggcatataca tatgccagcg acattgcacc aataatagag
     6961 ataatccaac cccagatagc aataccaccg atacttgcta ggttcgcagg taataatgca
     7021 ataccgctcc ccatcatatt accggcaaca acaccggtac aggcaaatag cccgatcttc
     7081 ttggcagaac tcatgctctt ctcctaattt catttttgaa tttggagtcc gggtcatgat
     7141 gtataactat ttcctgacca gaccaaactg gcgctaagat tactcacgaa aaaaggatta
     7201 atcctaaaga ttaggtgaaa taacacaaaa gtttctgtaa gtgagaactt gaggtttttt
     7261 attaacacat caggatcgca agttgatatc atgaaaagat aaacattcaa tgtttacaat
     7321 gcattgcgtg acattctctg gttaaattta tgtaataaaa attatgcggc aaataaattg
     7381 ccgcaacata ttataccaac gggaacatac aaaaactcaa caacaaatat tcccgagcat
     7441 gaatcaaccc gggagttact tattctgaag cgagaaattt gtcgagataa ggtacaacat
     7501 aaggaacaga agtctggaat ataccatttt caatccagta aagggtgttt gcccctgggc
     7561 gtaaattaaa ggcggtgaga tatgcatcag ctgcttcccg gttcatcccc ttcatttcat
     7621 aaaccttgcc aagcaacaca taatttagcc aggacatttc aagatcaatg ccggtattta
     7681 tcgcctgata agactcatct gttttacctt ttaccagggc actgaccgct tttatttgat
     7741 atataatgga caggttgttc aattccggca gtgtaacaat gttatctatt tctgtgttca
     7801 gtgctgctaa ttgtttttcg tctaaaggat gttgagaatg gcgcacgata tcaactaatg
     7861 ctttttctgc tctcgcgtag gtaaattctg gggatgattg aacaatctca cctaataatt
     7921 cactggcacg atccaatgat ttatcatcac catgcagtaa ataatcatgt gcctgataaa
     7981 aattagttaa taacgcacca cgatgcggca aaatttgctg gagcatctcc tgcattcgtt
     8041 gtggccacgg ttggtttaac gcttttgata aactctggag caaatcattt tgaatcgcca
     8101 gctgattacc gttagtgatg acataacgtt tatccagcat ggttgaacca tctgcattgt
     8161 ctaccaattt taccgacata aagcattgtt gagcacggta ttggcgctga ttaacaaacg
     8221 caatagataa tgttttaccg gaactgctcg gttcatcaat gttgtagttg attttgtcat
     8281 gcaccataaa ggtggagaag gtgttaagtg atgtcgccac caaatcaccc acgcctatcg
     8341 cgtaagagag ctgatacgga gaactccagc tgttacaact cttattaacc atattaatgt
     8401 caatatcgcg tggattgagc aaaatgcgcg atttactcat aggaagacgt gtttcaagac
     8461 ttgaaaacgc taccagtgcg acacagatac ctaacgacaa caggaaaaaa aaccataccc
     8521 aaaaggtagt gaatcgtttg cttttaactg gggattgttc aggtggcgtt gtggtgtttt
     8581 gaatgttaag gctgtgggag ggagaatctg tggcaggaac cgcctctggt atagggggag
     8641 gcgaagatag cattatttcc tctccctctt cttcgctgta ccagataacc ggcaccatta
     8701 atttatagcc gcgctttggt acagtagcga tatagacagg actatcttca tcattatctt
     8761 ttaatgactt acgtagttct gagatactct gcgtcacaac gtgattggtg acaatacttc
     8821 tcttccagac attatcgata agttcatccc tgctaagtac ttcgccactg tgttgagcaa
     8881 agaaaaccag aagatcgatt aatctcggct caagggtaag ttgacgccca ttgcggctaa
     8941 tttggtttat ggacggagta acaagccatt cgccaacgcg aactacaggt tgttgcataa
     9001 tagaaactca ttcgaaaagg gaatgatgca atgataattg ccataaccta ttttttccat
     9061 ctatagatgg gtttatttac atattattgg tgaatgcaaa acgttatttt tactcgccat
     9121 aaacttcttg attacatagt attacgaaag gattttactg agaaccagaa gtaattttcc
     9181 ttaccatcaa aattcatcat ctttgccaaa gaaaaatgtt cagaaaataa tccatggaaa
     9241 attgtccgga gcgcttacta ttttaatgga ttgttagtct ttgcatgagc aaacggactg
     9301 atacatttct ctttgttctc attcagaaaa tctcatcagt tggcgttctg cccgatgttg
     9361 tgctttatgg gtgcgttacg ctcctgatga cgtcatttga cgttcaacag catcacgggg
     9421 ccgcacgaca tttcacgtca gtcagcgcta tagctcagaa acaaattttc ccgaattggg
     9481 atatgcccgc aaattgctgg tgatgtgggg agaatctggt tgagttcggt agaattgatt
     9541 tggcggcagg acgcttaaat cgtggcgtcc tgaaacgaaa aacggacctc cgtggaggtc
     9601 cgtttatatg aat
//
LOCUS       DADTFG010000173         9594 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.174, whole genome shotgun sequence.
ACCESSION   DADTFG010000173 DADTFG010000000
VERSION     DADTFG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.174"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            200..1357
                     /gene="acrE"
                     /locus_tag="J1138_004076"
     CDS             200..1357
                     /gene="acrE"
                     /locus_tag="J1138_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBA8455807.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADATVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1369..4473
                     /gene="acrF"
                     /locus_tag="J1138_004077"
     CDS             1369..4473
                     /gene="acrF"
                     /locus_tag="J1138_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HBA8455808.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIILVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTILTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4726..4947
                     /locus_tag="J1138_004078"
     CDS             4726..4947
                     /locus_tag="J1138_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455809.1"
                     /translation="MKRLISVTLLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5378..6403
                     /locus_tag="J1138_004079"
     CDS             5378..6403
                     /locus_tag="J1138_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8455810.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGVNSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6471..7652
                     /locus_tag="J1138_004080"
     CDS             6471..7652
                     /locus_tag="J1138_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA8455811.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQIFAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            7662..8765
                     /locus_tag="J1138_004081"
     CDS             7662..8765
                     /locus_tag="J1138_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA8455812.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSLSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPYRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8773..9531
                     /locus_tag="J1138_004082"
     CDS             8773..9531
                     /locus_tag="J1138_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBA8455813.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT         2287 a         2281 c         2469 g         2557 t
ORIGIN      
        1 tttaaatccg cgtcaaaata aaacaatagg atattaatct ttttttgtgt ttatgtgcct
       61 tgagtcgcca gtattcataa ctattcctta catcgacgaa tgataatttg taggatagcg
      121 aactgtattt ttctttctgc gagttaacgc attgcctttt tgggcaaata acgcgctttt
      181 ggttttttga ggaatagtaa tgacgaaaca tgccaggttt ttcctcctgc cctcctttat
      241 tctgatctcc gcggctttaa tcgccggttg taacgataag ggagaagaga aagctcacgt
      301 cggtgaaccg caggttaccg ttcatattgt gaaaacggcc ccgttagaag ttaagactga
      361 attaccaggc cgcaccaatg cttaccgtat agccgaagtt cgcccacagg ttagcgggat
      421 cgtactgaat cgcaatttca ctgaaggcag cgatgtgcaa gcaggccagt ccctgtacca
      481 gatcgatccc gcgacctatc aggcaagtta tgacagcgcg aaaggcgaac tagcgaaaag
      541 tgaagccgcc gccgccatcg cgcatttgac ggtaaaacgt tacgttccgc tcgtgggtac
      601 gaaatacatc agtcagcagg agtacgacca ggccattgct gatgctcgcc aggccgatgc
      661 caccgtgatt gccgcaaaag ccacagttga aagcgctcgc atcaatctcg cttataccaa
      721 agtgactgcg cctattagcg gacgtatcgg caaatcgact gtgaccgaag gcgctcttgt
      781 cactaatggg caaacgactg aactggcgac tgtccagcag ctcgatccta tctacgttga
      841 tgtgacccaa tccagcaacg attttatgag gctgaagcaa tccgtagagc aaggaaattt
      901 gcataaggaa aacgccacca gcaacgtaga gttggtcatg gagaacggtc aaacctatcc
      961 cctgaaaggt acgctgcaat tctccgatgt gaccgttgat gaaagcaccg gctccataac
     1021 cctacgtgct gtcttcccta acccgcaaca tacgcttttg ccgggtatgt ttgtgcgcgc
     1081 acggattgat gaaggcgtcc aacctgacgc cattcttatc ccgcaacaag gcgttagccg
     1141 cacaccacgt ggtgatgcaa ccgtgctgat tgttaacaat aaaagtcagg ttgaagcgcg
     1201 ccctgtcgtt gccagtcagg cgattggcga taaatggttg attagcgaag gtctgaaatc
     1261 tggcgatcaa gtcattgtca gcggcctgca aaaagcgcgt ccgggagagc aggttaaagc
     1321 cactaccgat acccccgcag atactgcatc gaagtaaggc aatctgacat ggcaaacttt
     1381 tttattcgac gaccgatatt tgcatgggtg ctggccatta ttctgatgat ggcgggcgca
     1441 ctggcgatcc tacaattgcc cgtcgcccag tatccaacca ttgcgccgcc tgcggtttct
     1501 gtttcggcaa actatccggg tgctgatgcg cagaccgtgc aggatacagt gacgcaggtt
     1561 atcgaacaga atatgaacgg tatcgataac ctgatgtata tgtcctccac cagcgattcc
     1621 gccggtagcg tgacaattac ccttaccttc cagtccggga ccgatcctga tatcgcgcaa
     1681 gtacaggtgc agaacaaact ccagctcgcc acgccgttgc tgccgcagga ggttcagcag
     1741 caggggatca gtgttgaaaa gtccagtagc agctatttga tggtggcggg ctttgtctct
     1801 gataaccctg gcaccacaca ggacgatatc tcggactatg tggcctctaa cgttaaagat
     1861 acgcttagcc gtctgaatgg cgtcggtgac gtacagcttt tcggcgcaca gtatgcgatg
     1921 cgtatctggc tggacgccga tctgctaaac aaatataaac tgacaccggt tgatgtgatt
     1981 aaccagttga aggtacagaa cgatcagatc gctgccggac agttgggcgg aacgccagcg
     2041 ttaccagggc aacagttgaa cgcctcgatt attgctcaga cgcggttaaa aaatccggaa
     2101 gaattcggca aagtgaccct gcgcgtaaac agtgacggct cggtggtacg cctgaaagat
     2161 gtcgcacggg ttgaacttgg cggtgaaaac tataacgtta tcgctcgtat caacggaaaa
     2221 ccggcggcgg gcctggggat taagctggca accggcgcga atgctctcga taccgcgaaa
     2281 gccattaagg caaaactggc ggaattacag ccattcttcc cgcagggaat gaaggttctc
     2341 tacccttatg acaccacgcc tttcgtccag ctttctatcc aggaagtggt gaaaacgctg
     2401 ttcgaagcca ttatcctggt attcctagtg atgtatctgt tcttgcagaa catgcgggca
     2461 acattgatac ccaccattgc tgtacctgtc gtgttgttag ggacatttgc catactcgcc
     2521 gcttttggtt actccatcaa tacgctaacg atgtttggga tggtacttgc catcggactt
     2581 cttgttgatg atgcgatagt cgttgtggag aacgtcgagc gcgtaatgat ggaggataaa
     2641 ctccccccca gagaagcgac ggaaaaatcg atgtcgcaaa tccagggcgc gctggtaggt
     2701 atcgcgatgg tactgtcggc ggtatttatt ccgatggcat tcttcggtgg ctcaacaggg
     2761 gcaatttatc gacagttctc tatcactatt gtttcagcaa tggcgctttc agtactggtt
     2821 gcgctgattc tcacccccgc gttatgtgca acattgctga aaccagtctc tgccgagcat
     2881 cacgaaaata agggaggatt cttcggctgg tttaacacca ctttcgatca tagcgttaat
     2941 cactacacca atagcgttgg caaaatcctc ggttccacag ggcgatattt actgatctat
     3001 gcgctgattg ttgcaggaat ggtggtgttg tttttacgtc ttccgtcttc cttcttacct
     3061 gaagaggatc agggtgtctt tctgaccatg attcagttac ccgctggcgc gacgcaagag
     3121 cggacgcaaa aagtgttgga tcaagtgacg gattactatc tgaagaacga gaaagcgaac
     3181 gttgaaagtg tctttacagt taacggcttt agcttcagcg gccaggcaca aaacgccggt
     3241 atggccttcg tcagtctgaa accgtgggaa gagcgtagtg gtgacgaaaa cagtgcggaa
     3301 gcagtaatcc atcgtgcaaa aatggaattg ggcaagatcc gcgacggttt tgtcattcca
     3361 tttaatatgc cagccattgt tgaactaggc acggcaacgg gtttcgactt tgagttaatt
     3421 gatcaggctg ggctgggtca cgatgccctg acccaggccc gtaaccagtt gcttggcatg
     3481 gcggcgcaac atcctgccag cttagtcagc gtgcgcccta atggcctgga agacaccgcg
     3541 cagtttaaac tggaagttga ccaggaaaag gcgcaggcat taggtgtttc actttctgac
     3601 atcaatcaga ccattttaac ggcgttgggt gggacttacg ttaacgactt catcgaccgt
     3661 ggtcgcgtga aaaaggtgta tgttcaggcg gatgccaaat tccgtatgct gccagaagat
     3721 gtcgataaac tttatgtccg cagcgccaac ggcgaaatgg tgccattctc tgcctttacc
     3781 acttcacatt gggtgtatgg ctctccgcga ctggaacgct acaacggtct gccgtcaatg
     3841 gagattcagg gggaagccgc gccaggaacc agttccggcg atgctatggc gttgatggaa
     3901 aaccttgcgt caaaattacc ggcgggcatt ggttatgact ggacgggtat gtcatatcag
     3961 gaacgtttat cgggaaacca ggctcccgct ctggtagcaa tttcctttgt ggttgttttc
     4021 ctgtgccttg ctgcactcta tgaaagctgg tcaattcctg tctcggttat gttggtcgtg
     4081 ccgttaggga ttgtcggcgt gctactggcg gcaacactct ttaatcaaaa aaatgacgtc
     4141 tactttatgg tgggcttgct aacgacaatt ggcttgtcgg ccaaaaacgc tattttgatt
     4201 gttgagttcg ctaaagatct catggagaaa gagggtaaag gtgttgttga agcgacacta
     4261 atggcagtac gtatgcgtct gcgtcctatc ctgatgacct ctctcgcctt tattctcggc
     4321 gtattaccgc tggctatcag taacggggcc ggcagtggcg cgcaaaacgc agtcggtatc
     4381 ggggtaatgg gcggaatggt ctctgcaacg ttgctggcaa tcttcttcgt gccagtgttc
     4441 tttgtggtga tccgtcgatg ctttaaagga taaatcagaa acataaaggc gctttcaggc
     4501 gcctttttta ttttcagtga accccataaa aattaataag atattcttct tctcactctt
     4561 taaaaacttt ctatagttcc cgctcccttc actattttta caattcacat aaattgtcat
     4621 tattcattcc gcaagattta taacctccag tgataaaatg tcattcagct cgttaataag
     4681 agagttaact tattaagcgt tagcgtttat tactgaggta acaccatgaa aagattaatt
     4741 tctgtgacat tgctcaccgc attgctggcg ggctgtgctc acgattcgcc ctgcgttccg
     4801 gtttatgacg accagggtcg tctggttcac accaatacct gtatgaaagg cacgactcag
     4861 gataactggg aaacggctgg ggctattgcc ggcggggcag cagctgttgc tggcctgacg
     4921 atggggatca tcgctctgtc gaaataacta aacagggcgc ggagctccgc gctcttttta
     4981 acgatacagt atctttccat aagataaaat attgctatat tctgctgggt aattccctgc
     5041 tgatttagca tgttatatca cgttatttcg ctgctttcga aataacgcat caatttaact
     5101 ttcacacatc ccaccagaaa tatactcacg ctaacaatca ttattctgct tagtaaaaaa
     5161 cgcgtaaata ttttgcgcca aaatgtggcg catgtttcat tttcgcacca ttgcggggcg
     5221 ctgtttttat tatttcctgt ctacactcag cttattgcga cgcgaaatcg tgcgcaaaag
     5281 ctggcactac ttttgcttat aagaggatgg ccacagacag gtaaaagacg tttcccaaac
     5341 gtcctataac gataattttc gcctcacagg aagcattatg aaaaagatga tgatagccac
     5401 actggctgcc gccagcgtgc tgcttgccgt tgcaaatcag gcgcatgctg gcgcgacgct
     5461 tgatgccgtt cagaaaaaag gttttgtgca atgcgggatc agtgatggat tacctgggtt
     5521 ctcttatgcc gatgctgacg gtaagttttc aggtattgat gtagatgttt gtcgtggcgt
     5581 tgccgctgct gtattcggtg acgatacgaa agtgaaatat accccgctca ctgcaaaaga
     5641 acgcttcacc gctttacagt caggggaggt ggatttgctc tcccgtaata cgacctggac
     5701 ttcatctcgc gatgccggga tgggaatggc atttactggc gtcacttatt acgacggcat
     5761 tggcttcctg acgcacgata aagcggggct aaaaagcgcg aaagaactgg atggcgctac
     5821 cgtctgtatt caggcgggta ctgatactga actcaatgtt gccgattact tcaaggcaaa
     5881 caatatgaag tacacaccag tgactttcga tcgctctgac gaatcagcga aggcactgga
     5941 atctggtcgc tgcgatacgc tagcctcgga tcaatcacaa ctgtatgccc tgcgcatcaa
     6001 attaagcaac ccagctgaat ggattgtctt accggaagtt atctctaaag aaccgcttgg
     6061 gccggtagtt cgtcgtggcg atgatgaatg gttctcgatt gtacgctgga cgcttttcgc
     6121 catgctgaat gctgaagaga tgggcgttaa ctcgcaaaac gtcgatgaaa aagcggctaa
     6181 tccagcaacg cctgatatgg cacatctgct gggtaaagag ggcgattacg gcaaggatct
     6241 gaagctggat aataaatggg cttacaacat tatcaaacag gtgggtaact actcggaaat
     6301 ttttgagcgt aacgtaggtt cagaaagccc gctgaaaatt aaacgtgggc aaaataatct
     6361 ctggaataac ggcggtattc agtacgcgcc gcccgtgcgt taagtcggtg tgaggtaacg
     6421 agcgtcgcta cggcggcgct ctcttcagcg ttatgctttc cgaggtttct atgtctcatc
     6481 gccgctcaac cgttaaaggc tcactctcct ttgccaaccc tacggttcgc gcctggttat
     6541 tccagatctt tgccgttgtt gctgttgtcg gaattgttgg ttggctattt cacaacactg
     6601 tgacgaatct cagtaatcgt ggcattactt caggttttgc ctttctggat cgcggcgctg
     6661 gcttcggtat tgtccagcat ttgatcgatt accagcaggg tgatacctac ggacgcgttt
     6721 tcattgttgg cttactcaat acgctactag tttctgcatt gtgtattgta ttcgcttctg
     6781 ttctgggctt ctttatcggt ctggcgcgac tttcggataa ctggctgcta cgaaagcttt
     6841 ccacaattta tattgagatc ttccgtaata ttccgccgct gctgcaaatc ttcttctggt
     6901 actttgccgt gttgcgcaat ttgcccggac cacgccaggc tgtgagcgcg tttgatctgg
     6961 cctttttgag caatcgtggg ctttatattc cgtcaccgca gctgggagac ggatttattg
     7021 cgtttatcct ggctgttgtt atggctatag tcctttctgt tgggctattc cgatttaata
     7081 aaacatacca gataaagacc ggacagctgc gccgcacctg gccgatcgcc gcagtgttga
     7141 tcattggttt gcctttactg gcgcaatggc tttttggcgc agcactacac tgggatgtcc
     7201 cagccctacg aggctttaac ttccgcggcg ggatggtttt aattcctgaa ctggcagcct
     7261 taacgctggc actttcggtt tatacctctg catttatcgc cgagattatc cgcgctggga
     7321 tccaggcagt gccttatggt caacatgaag ctgctcggtc actgggatta cccaacccgg
     7381 ttacgctacg ccaggtcatt attccccagg cactgcgggt gattattcca ccgttaacca
     7441 gccagtatct caacatcgtc aaaaactcct ctcttgccgc cgcgattggc tatcccgata
     7501 tggtttcgct gtttgctggc accgtgctga accagacggg gcaagccatc gagacgatag
     7561 ccatgaccat gtcggtctat ctgattatca gcctgactat ctcgctgctg atgaatatct
     7621 ataaccgccg catcgcgatc gttgaacgct aaggagtcat gatgacaaaa gtattgctgt
     7681 ctcatccccc gcgcccggcg agccataact caagccgcgc gatggtgtgg gtgcgaaaaa
     7741 atctgttctc cagcttgagc aatagcctac tgactattgg ctgcatatgg ttgatgtggg
     7801 aactgatccc accgttgctg aactgggcat ttttgcaggc taactgggtt ggctcaacgc
     7861 gtgccgactg cacaaaagcc ggtgcctgtt gggtcttcat ccacgaacga tttggtcagt
     7921 ttatgtatgg gctttaccca cacgaccaac gctggcgaat taacctcgca ttactgattg
     7981 ggcttgtgtc gatcgcacca atgttctgga aaatactccc gtatcgcggt cgctatattg
     8041 cggtatgggc ggtgatttac ccactgattg tctggtggct gatgtatggc gggtttcttg
     8101 gtcttgagcg ggttgaaacc cggcaatggg gcgggctgac gctaacttta attattgcat
     8161 cagttgggat tgcgggggcg ctgccgtggg ggatcttact ggcgttaggt cgccgctccc
     8221 atatgccgat tgtgcgtatc ttatcggtca tttttatcga gttctggcgc ggcgtaccgc
     8281 tgattaccgt attgtttatg tcttcggtca tgctgccgtt gtttatggca gaaggcacca
     8341 gtatcgacaa attgatccgc gcgctggttg gcgtgatcct gtttcagtca gcatatgttg
     8401 cggaagtcgt gcgaggcgga ttacaggcgc tgcctaaagg gcaatatgaa gcggcagagt
     8461 cgctggcgtt gggttactgg aaaacccagg ggctggttat tctgccacag gcgttgaagc
     8521 tggtgattcc tgggctggta aataccatca tcgcactctt caaagatacc agcctggtga
     8581 tcatcatcgg gttgttcgat cttttcagta gcgttcagca ggcaaccgtt gatcccgcct
     8641 ggttgggtat gtcgacggaa gggtatgttt tcgccgcgct gatctactgg atcttctgtt
     8701 tcagcatgtc acgttatagc caacatctgg aaaaacgttt taacaccggg cgtacaccgc
     8761 attgaggaca ctatgagcca aattttactg caacctgcta acgcgatgat tacgctggaa
     8821 aacgtcaata aatggtatgg acaattccat gttttgaaaa atataaattt aaccgtgcaa
     8881 ccgggagaac ggatcgttct gtgtggccct tctggttccg gtaaatcgac aaccattcgt
     8941 tgtattaatc atctggaaga acatcaacag ggacggatcg tggtggatgg catcgaactt
     9001 aatgaagata tccgcaatat tgagcgcgtc aggcaggaag tgggaatggt ctttcagcat
     9061 ttcaatctct tccctcatct gaccgttcta cagaactgta ccctggcacc gatttgggta
     9121 cgcaagatgc ctaaaaaaga ggctgaagct ctggcgatgc attaccttga gcgggtgaga
     9181 attgccgaac atgcccagaa gtttcccgga cagatttctg gtggtcagca gcaacgcgtt
     9241 gccatagcac gttcactttg tatgaaacca aagatcatgc tatttgatga acctacgtca
     9301 gcgctcgatc cggagatggt gaaagaagtg ctggatacga tgattgggct ggcgcagtcg
     9361 ggtatgacaa tgttgtgtgt aacacatgag atggggtttg cacgaaccgt cgctgacagg
     9421 gtgattttta tggatcgtgg ggagatagtg gaacaagctg caccggatga gttttttgcg
     9481 catcctaaat cagagcgtac gagggcattt ttatcgcagg tgatccatta attgaatgtt
     9541 agttcgaaaa gcaaaaaggc catcctttcg gatggccttt cgcttgattt gatg
//
LOCUS       DADTFG010000174         9332 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.175, whole genome shotgun sequence.
ACCESSION   DADTFG010000174 DADTFG010000000
VERSION     DADTFG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.175"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(164..955)
                     /gene="cpoB"
                     /locus_tag="J1138_004083"
     CDS             complement(164..955)
                     /gene="cpoB"
                     /locus_tag="J1138_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBA8455814.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(965..1486)
                     /gene="pal"
                     /locus_tag="J1138_004084"
     CDS             complement(965..1486)
                     /gene="pal"
                     /locus_tag="J1138_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBA8455815.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="J1138_004085"
     CDS             complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="J1138_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBA8455816.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2946..4211)
                     /gene="tolA"
                     /locus_tag="J1138_004086"
     CDS             complement(2946..4211)
                     /gene="tolA"
                     /locus_tag="J1138_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBA8455817.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4276..4704)
                     /gene="tolR"
                     /locus_tag="J1138_004087"
     CDS             complement(4276..4704)
                     /gene="tolR"
                     /locus_tag="J1138_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBA8455818.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4708..5400)
                     /gene="tolQ"
                     /locus_tag="J1138_004088"
     CDS             complement(4708..5400)
                     /gene="tolQ"
                     /locus_tag="J1138_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBA8455819.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5397..5801)
                     /gene="ybgC"
                     /locus_tag="J1138_004089"
     CDS             complement(5397..5801)
                     /gene="ybgC"
                     /locus_tag="J1138_004089"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBA8455820.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5951..6244)
                     /gene="ybgE"
                     /locus_tag="J1138_004090"
     CDS             complement(5951..6244)
                     /gene="ybgE"
                     /locus_tag="J1138_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBA8455821.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6244..6357)
                     /gene="cydX"
                     /locus_tag="J1138_004091"
     CDS             complement(6244..6357)
                     /gene="cydX"
                     /locus_tag="J1138_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBA8455822.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6372..7511)
                     /gene="cydB"
                     /locus_tag="J1138_004092"
     CDS             complement(6372..7511)
                     /gene="cydB"
                     /locus_tag="J1138_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBA8455823.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7527..9095)
                     /gene="cydA"
                     /locus_tag="J1138_004093"
     CDS             complement(7527..9095)
                     /gene="cydA"
                     /locus_tag="J1138_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBA8455824.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2298 a         2540 c         2223 g         2271 t
ORIGIN      
        1 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
       61 ttcagacttg agcgcaacaa aaatttcgat gtagatcact caactgctta tgattcgcac
      121 gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc gcgttcagac
      181 gtttttgcgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      241 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat aacgccgact ttaaacatcg
      301 catctgcagc cttcggtgac ttcggatagt ttttcactac cgaagcaaaa taatacgccg
      361 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      421 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      481 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcaccgc
      541 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
      601 ccacaccgct ttgatcgccg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
      661 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
      721 cctgaatctg accacgcagg gaatcaatat cggattgatt atcagagagt tgttgctgga
      781 gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg acgcggtctt
      841 cgaccgagcc tgagccgaca ctactgattg gtgcctgagc aaaagcggcc cagggggccg
      901 ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg ctcatgcaat
      961 tctcttagta aaccagtacc gcacgacggt ttttggcata cgccgcttcg tcatgaccca
     1021 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtcagca gaaacgcctt
     1081 taccctgcag atacatctta acagcgttcg cacgacgttc acccagggag atgttgtatt
     1141 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1201 gcaggaagtt tgcgtgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1261 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1321 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg gcacccagca
     1381 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1441 caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1501 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1561 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     1621 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     1681 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     1741 atgtgctgct gcccaccgtt ggagctgacc attaccataa atttaccgtc gctgctgaca
     1801 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     1861 ttcactttat aaacctgcgg acgaccggcc tggtcagaag taaatgccag gttctggctg
     1921 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     1981 ccagaagcca aatccattac gtacaggttc aggctaccgg ttttcgacaa ggcgaatgcc
     2041 agtttgctgc cgtctggcga gaatgcaggt gcaccgttgt gacgcgggaa tgaagccacc
     2101 tgacgtacag cgccatttgc cagcgtctga ataaccagcg cggaacgacc gctttcgaag
     2161 gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag cggttgcggt
     2221 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2281 aactgaccgc cgttagtctg aacaacgtag gcaatacggg tacggaacgc acctttaata
     2341 ccggtcagtt tttcaaatac ttcgtcactg gcggtatgac cagcataacg cagccactgc
     2401 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2461 agttgataag caacattgta agaaccatcc ggattcggag tgacctgacc gacaaccaca
     2521 gcgtcaatgc ccagcgcgga ccatgcagct ggttgtactt cctgagcact acccggctgc
     2581 tgtggcagac gagcacgatc taacggatta aatttaccgc tgttacgcaa gtcagcagca
     2641 acgatgccgc caatatcttc aggtgccgca ccaggccccg cccactggaa aggaacaaca
     2701 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     2761 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     2821 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     2881 aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtcggaa cagtcaacat
     2941 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3001 ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcttgaca aagtgcggga
     3061 tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt tatgcgcagc
     3121 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3181 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3241 ctcccggcag gcgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3301 ccagagctta gctcaccgaa aatatcatct gcctcagcgg cagcttttgc tgcagcggct
     3361 ttttcagctg ctgcttttgc cgctgccgct tttttgtctg cggcggcttt ttctgccgct
     3421 gctttcttat cagctgctgc cttttcagca gccgcttttt ctgctgcttt tttgtcggct
     3481 gccgctttct ctgctgccgc tttcttatca gctgcagcct tttcagcagc cgctttcttc
     3541 tcagcttctg ccttggcttt ttcagcagct tcggttgccg ctttctttct tgcttcagct
     3601 gcagctgctt ctgccgcttc agctttcttc ttcagcgccg cagcagcagc ctcggctttt
     3661 ttctgcgctt cgactgcggc tttggcggct tctgcttctg cttttttctt cgcgtctgcc
     3721 gccgctttct tcgctgcttc ttccgcagct ttagcatccg cttcggcctt cgctttagca
     3781 tctgccgccg ctttcgctgc cgcctcttcc gcttgcttct gctttaactc ggcctgtttt
     3841 gcggcttctt cagcctgctt tttctgctcc tgagccgcta accgctcttt ctcaagttgc
     3901 ttcaggcgtt cctgttcagc cgcttgtttc tcacgcagtt cttcagcagc ctgctgctct
     3961 ttcatcttgc gctgctcatc agaacgcttc gcgcttgatt cctggctttg catgcgtttg
     4021 tactgctcaa ctaccgcacc tgaatcaacc atgacagcgt cgatggagga accaccgccg
     4081 cctccggctg aagcttctat attctcatcg aacgaactcc agatcagcgc cgcaaataag
     4141 atgacatgca gcactgctga aataattatc gcccgcttga gcttgtcgtt ttgttcggtt
     4201 gcctttgaca ctctcggttt ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa
     4261 gggaaacgca gatgtttaga taggctgcgt cattaaacca accgatttca cacccgcact
     4321 atgtaacaag ttcagtgctt taattatttc atcgtaaggc acatcttttg cgccaccgat
     4381 cagaaagacc gttttcgggt tggccttgaa acggctggac acttccgcca ccacctgctc
     4441 tggtggtaaa cgctccaggc gatctttctc aaccaccacg gtgtactgac caataccaga
     4501 cacttcaaca atcactggcg gattatcatt actgctcacc gcctgtgatt cagtagcgtc
     4561 tggcagatcg acctccacgc tctgggtgat gatgggcgct gtcgccataa agatcagcaa
     4621 cagcaccagc agtacgtcca gcaacggtac aatgttgatt tcggacttga gatcgcgacg
     4681 acctcgtcca cgcgctctgg ccatggctta ccccttgttg ctctcgctaa cggtaaacgc
     4741 ctggcggtgc agaatcgcgg taaactcttc cataaagttg tcgtaattca gttccagttt
     4801 gtttacgcgc tggttgaggc ggttataagc cattaccgcc ggaattgcgg cgaacaaacc
     4861 aattgccgtc gcaatcaacg cttctgcgat accgggcgca accatttgca gtgttgcttg
     4921 ttttaccgcc ccgagggcga taaaggcgtg catgatcccc cagaccgtac caaacagacc
     4981 aatatacggg ctgatggagc caaccgtacc gaggaaagga atgtgcgttt ccagattttc
     5041 aagttcacga ttcatggaaa tacgcatcgc acgcgacgcc ccttccacta cggcttccgg
     5101 cgcatggcta ttggcacgat gcaggcgcac aaactctttg aagccgctgt aaaagatttg
     5161 ttccgaaccg ctcagattat cccgtttccc ctggctctct tgatagaggc gagagagttc
     5221 gattccagac cagaatttat cttcaaacgc ttcggcttcg cgcgccgctg cgttaagaat
     5281 acgggtccgc tggataataa tggcccagga tgcgattgaa aaaccaatca aaatcaacat
     5341 gataagttta accagaaggc tagccttcag gaacaaatca aggatattca tgtcagtcac
     5401 tgcttaaact ccgcgacaat agacttggga agcgcacgag gcttcatttt gagtgggtca
     5461 acgcaaacaa ccagaacctc tgcttcattc aacaaagtat tctcggcgtt gacaatacgt
     5521 tgcgtgaaaa ccaaagaggt gccacgcatt gatgttattt cagtctgtat ttcgagcata
     5581 tcgtcgagcc gcgcaggtgc gtaatattcc accgtcattt tacgtaccac aaaggcaacg
     5641 cgttcagcca tcagcgcctg ttgactgaaa tgatgatgac gcagcatctc tgtgcgtgct
     5701 ctttcataaa aagcgacgta actggcgtgg tacaccacac caccggcatc ggtatcttca
     5761 tagtagacac gaaccggcca tcgaaacagc gttgtattca ctttacatcc cggtaatgca
     5821 acaaaagtta gagcttttaa acgctgctac tatacgcgcg cgattgaggt ttgggaatga
     5881 gggcaagtta agggagcgta aaaatttatg ggcctgactc ggcccataaa tttttaaaga
     5941 gaattctgat ttaaaagaag aagaaaatca gcccgacaat gagaacaata tcggcaagca
     6001 atgggcaaaa aatcccttgc cagagaacct tctgcggacg aaagcccacg ccgtgaatca
     6061 caccagcaca gacggcccac ataagcagta aaccatgcca gatttccagt tcactggtct
     6121 tcgcggcgaa acgtgatggg tcccaaaaca tacatcctgc taacagaagt gccatcacga
     6181 aggaaagcgc ccgcaggggg cgcttgtcca ttaccgcata caaggttgcg ataatcttac
     6241 tcatcagatg tcttcttgac cggctttgcc tgattcgacg tgctcaagcg ccagcgcggt
     6301 gattacccca aacgaacagg caagaagcgt tcccagaatc catgcgaaat accacatttt
     6361 tagctcctta cttagtacag agagtgggtg ttacgttcaa tatcttcttt ggtgatacga
     6421 ccgaacattt tccagtaaca ccaggcggtg tagagcagaa tgatcggtac cagaaccacc
     6481 gcaacccagg tcatgacgtt aagcgtcagc tggctggaag ttgcatccca cattgtcaga
     6541 cttgcgttca tcatggtgct ggacggcatc acaaacggga acattgcgat accggcagtc
     6601 aggatgatgc aggccagcgt cagggaggag aatacaaacg cccacgcggc tttatccata
     6661 cgtgcagtca ggatggtcag cagcggcaga accacaccca gtgccggaat agcccacaga
     6721 attggcgtgt tgttgaagtt aaccagccat gcgccagctt cacgaaccac ttctttattc
     6781 agcgggttag aggctgcgta atggtccatt gtcgatttca cgacataacc atcgataccg
     6841 tacatcaccc atacgccagc cagtgcgaag cagaccagcg tcaccagtgc cgccacctga
     6901 gccgttgcac gggtacgcag gtgcagttcg cccacggtac gcatttgcag ataggttgcg
     6961 ccctgagtaa tgatcatccc tacgctcacc acacctgcca gcaggccgaa cgggttaagc
     7021 agctggaaga agttaccggt gtagtacaga cgcagatatt catcaacgtt gaatggtacg
     7081 ccctgcaaca ggttaccgaa cgctacacca atcaccagcg gcggaacgaa gctaccaatg
     7141 aagatgcccc agtcccacat gttacgccag cgggtttctt caatcttgga gcggtagtca
     7201 aaaccgaccg gacggaagaa caaagacgcc agcacgagga tcatcgccac atagaagccg
     7261 gagaatgcag cggcatagac catcggccag gcagcaaaca gtgcgccgcc tgcggtgatc
     7321 agccaaacct ggttaccgtc ccagtgtggt gcgatggagt taatcataat tcgacgctcg
     7381 gtgtcgttac gaccgaggaa acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg
     7441 actgcaaaac caattagcag aacgccaacc agcagccacc agataaaacg caatacttca
     7501 taatcgatca tttgacgact cctgtcttag cgtgccggct gagtagtcgt ggaagactgc
     7561 tcaaagtgat agcgaccggt tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt
     7621 aagaacaatt ctgccaccag gaacagggta tacaggccgc aaatcagcac catcgagaag
     7681 atgagatcgc ctgcggtcag tgacgagttc gccacagctg tcggcagcac ttcaccgata
     7741 gcccacggtt ggcggccata ttcagccacg aaccagcccg cttctacagc aatccacggc
     7801 agcggaatac cgtacagcgc ggcgcgcagc agccattttt tctcgccaat gcggtttcgg
     7861 atgacactcc agaaagagag cgcgatgatt gccagcagca ggaagccaca cgccaccatg
     7921 atacggaacg caaagtacag cggcgctaca cgcggaatgg agtctttggt tgcctgttga
     7981 atctgagctt cagtcgcatc agccacgttt ggcgtatagc gtttcagcag cagaccgtaa
     8041 ccgagatctt tcttcatgct attgaactgg tcacgaacgg cctggtcagt agaaccagaa
     8101 cgcagttgtt cgagcagaga gtacgccttc atcccgttac gaatgcgttc ttcatgctgc
     8161 accatcagtt ctttcagacc gataactgga gtatccacgg aacgcgttgc aatgatgccc
     8221 agcgcgtaag ggatctggat cgcaaatttg ttcgtttcct cttcctgatc aggaatgccg
     8281 aacagagtaa aggcagcagg tgcaggttgc gtttcccact cggcttcgat agcagccagt
     8341 ttggttttct gcacgtcgcc catttcgtag ccggattcat cacccagaac aataacagac
     8401 agaacggcag ccataccgaa gctggcagcg atagcaaagg agcgtttagc gaaggcgaag
     8461 tcacgacctt tcagcatata ccatgcgctg ataccgagga tgaacattgc gccagtcaca
     8521 taaccagaag ctacggtgtg aacgaatttc acctgagcaa ccgggttaag caccagctcg
     8581 gagaagctca ccatctccat acgcatagtt tcaaagttga aatcggacgc gattgggttt
     8641 tgcatccagc cgtttgcaac cagaatccac agtgcggaca ggtttgaacc gagcgccacc
     8701 agccaggtga cacacatatg ctgaacttta cccagacgat cccaaccgaa gaagaacaga
     8761 cctacaaagg tggattcgag gaagaaggcc atcagacctt cgattgccag cggcgcaccg
     8821 aagatatccc ctacatagtg ggaatagtaa gaccagttag tcccgaactg gaactccatg
     8881 gtcagaccgg tagccacacc cagagcaaag ttgataccaa acaacttgcc ccagaacttg
     8941 gtcatatctt tataaatctg tttgccggaa aggacgtaga ccgtttccat aatggccagc
     9001 aggaacgcca taccgagcgt cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca
     9061 aactgtaagc gcgacagttc gactatatct aacatcatga ctccttgctc atcgcatgaa
     9121 gactccgaga gtgaaccccg ttagaaaggg tcacacgcat gccccaatac aaaattattt
     9181 gctcgcctct atcgttgcca tttcatcaaa agaggaaaag acaatgataa aaaagttaca
     9241 aatgtgttaa tgaataaacc aaattgatcc cgtaattata ttacgccgca aaatccttac
     9301 aataaacagg tttttattga gcaaatttgc at
//
LOCUS       DADTFG010000175         9306 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.176, whole genome shotgun sequence.
ACCESSION   DADTFG010000175 DADTFG010000000
VERSION     DADTFG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.176"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            254..538
                     /locus_tag="J1138_004094"
     CDS             254..538
                     /locus_tag="J1138_004094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455825.1"
                     /translation="MMWPEACFMLLQSSLYSLLDGEQVLFSFWELNKKQYEGFEFYQI
                     FSGNIVASCSSCSGVSSNSSAHYARCTDFMRHYSLNNYAACQLGRQACLS"
     gene            1002..1078
                     /locus_tag="J1138_004095"
     tRNA            1002..1078
                     /locus_tag="J1138_004095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1036..1038,aa:Arg,seq:tct)
     gene            complement(1357..2634)
                     /locus_tag="J1138_004096"
     CDS             complement(1357..2634)
                     /locus_tag="J1138_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBA8455826.1"
                     /translation="MVLSDCYSWDNEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSQSTAQVEGAYRLIRNPSVSPQAIAEAGFTATVRACEAHPLLLALEDTTTINFSHS
                     TASDDQGNTTTNPKTHGLLAHSVLMYAPDSALPVGFIEQQRWSRVTDTYGVKHQRKER
                     PCEEKESYRWQQASERMAERLGEIQKRVITVCDREADIWHYLHYKVSHGQRFVVRAAQ
                     NSRLEEAPGKLFELPEVLATAGSHTLNVMQKGGRAARQARMFIRYSEVSIKIATTVAS
                     AADARRIVSHYERRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIKAFIAVRVLG
                     LRQEGISEETQNDSCKKILTPTEWKLLWVKLEGKQLPSQTPTLKWACLKLGRWHDSKR
                     TGRPGWVVMWDGWFRLQDMVEGYPVMKSLDQEI"
     gene            2980..4950
                     /locus_tag="J1138_004097"
     CDS             2980..4950
                     /locus_tag="J1138_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA8455827.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMVKDTITIVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGEHAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNSTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYVDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHRGVYAGWRAELSASATHVKKGDPHQAGYTIHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            4969..5760
                     /locus_tag="J1138_004098"
     CDS             4969..5760
                     /locus_tag="J1138_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HBA8455828.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRDFFENPRLYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLS
                     DISRFSENVKLKVLYKTSPLAGAIMELDSVSYLTSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
     gene            6024..7223
                     /locus_tag="J1138_004099"
     CDS             6024..7223
                     /locus_tag="J1138_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HBA8455829.1"
                     /translation="MNRIPVIIINGFLGAGKTTLMKNLLTQANRNHLAVSVIVNDMSD
                     LDVDGVLIANTEIVNIANNNFVSITSDSISSQSGIKTVDKAIKNMLRHYLPDVIFVET
                     SGGSHPLPLIKYLQTHERLQLKSLLSLVDTVMLNDDYNGGKALIPAFQENLFHGKRRI
                     ENLLAEQIMFCSTLLLTKKDRLSFDIVTDVAKAIHPLNPYVNVIAVSWGNLKLAELLT
                     LPDYDFNRVGLLIRELEDTITVEEAKISAQNGEIISRVIKDDRPFHPQRLWETYHYFM
                     GMGIYRSKGFFWLPGRPDMALLWNQAAGSINLEFISYWNSGVLADPDNHLTHEERSVL
                     QKKVNKTMGRFGDRRCHLTIIGRSDEVHDFTSALINCFLSEEEIVWWQSGGVFADPWP
                     VNISRLN"
     gene            complement(7330..7515)
                     /locus_tag="J1138_004100"
                     /pseudo
     CDS             complement(7330..7515)
                     /locus_tag="J1138_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            7576..7896
                     /locus_tag="J1138_004101"
                     /pseudo
     CDS             7576..7896
                     /locus_tag="J1138_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            8173..>9306
                     /locus_tag="J1138_004102"
     CDS             8173..>9306
                     /locus_tag="J1138_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455830.1"
                     /translation="MGKIKYWLIVGFIILFAIFYIAISDRDSTLSRLKSAGENGDVEA
                     QYALGLMYLYGEILDVDYQQAKIWYEKAADQNDPRAQAKLGVMYANGLGVNQDYQQSK
                     LWYEKAAAQNDVDAQFLLGEMYDDGLGVSQDYQHAKMWYEKAAAQNDERAQVNLAVLY
                     AKGNGVEQDYRQAKSWYEKAAAQNSPDAQFALGILYANANGVEQDYQQAKDWYEKAAE
                     QNFANAQFNLGMLYYKGEGVKQNFRQAREWFEKAASQNQPNAQYNLGQIYYYGQGVTQ
                     SYRQAKDWFEKAAEKGHVDAQYNLGVIYENGEGVSQNYQQAKAWYEKAASQNDAQAQF
                     ELGVMNELGQGESIDLKQARHYYERSCNNGLKKGCERLKELLYK"
BASE COUNT         2561 a         1999 c         2263 g         2483 t
ORIGIN      
        1 ttaagagaga ttacttcacc agacaacttg aactcgctgc agggcgtttt ttatgcccga
       61 aaagctgcac agtacgttaa atatgctggt gattgtgcag tgagtttctc caagcctgat
      121 gtgacttgac tgaatgatat cacatttgta atgaaaaata aatcaatgtt tttaagttgc
      181 taggtgcttg tttctatagc tggggcactt ccttttacta tggctttcca tggtgggggc
      241 ggagctggtg ttgatgatgt ggccagaagc ctgttttatg ctgttacagt catcgttata
      301 ttccttattg gatggggagc aggtattgtt ttctttctgg gaattaaaca aaaaacaata
      361 tgaaggattt gagttttatc agattttttc tggcaatatt gttgcttctt gttcctcctg
      421 ctctggagtt tccagtaatt catcggcaca ttacgccagg tgcactgact ttatgcgtca
      481 ttacagcctt aataattacg ctgcttgcca gcttgggcgg caggcttgtc tcagttaaat
      541 gtatttcgga gatttcgttt attcgtcgtc accggattga gtgcatgatg gcgggattta
      601 tgatttattt ctggacattc tctcttattg ctggttggta caagccccag tttaagaagg
      661 aatatcagac actccactat gagaatgcta ggtattacgt tcttgcacgc tatgacggtc
      721 gtttggtttt atcacaatcg tacaggagcg ggagtaggaa gtttgttata atcaatggcg
      781 gacatagact ttgagattaa tgtagtcaga gtgcgttaaa attcccttag aaatcgggaa
      841 taggtagtac tagatacgca ggatttaatc agtcgtattg agatggaatt gcagagttaa
      901 actggcatgg attattttgt acccagccct taagaacaat ggtgagagat agtaactcat
      961 aatcgccaat tcactgtttt aaatttagta agggccgcta tccgccactg gctcatcggg
     1021 aaagagcatc agccttctaa gttgactgtg cgaggttcga gtcctcggtg gcggtccagt
     1081 actgatttag ctcagtaggt agggaattgc cttgtaatca gtaggtcacc agttcgactc
     1141 ttgctgtctc gccccatatt ccaataggct cgctttgacg gggcttttgt atgtcgcttg
     1201 cgatatgtta cagtaccgtt atcagtctgt agaaaataca cacaggagtg aaatgttaaa
     1261 ttacccacaa ctcagagagc ggagtgttgt cctgttagtg gggaatttgt taaaagaatc
     1321 cagcatggtg aatctccctg tgcggctgtc tcttgatcag atctcctgat caagagactt
     1381 catcaccgga taaccctcaa ccatatcctg aagtctgaac cagccatccc acataactac
     1441 ccaaccaggg cggcctgtgc gtttgctgtc atgccatcgc cccagtttca gacaggccca
     1501 tttcagtgtc ggcgtctgtg acggaagctg ttttccttcc agcttaaccc acagcagttt
     1561 ccactctgtc ggcgtcagta ttttcttaca gctgtcattt tgtgtttctt cactgatacc
     1621 ttcctgccgc aggcccagca cccgtaccgc gataaacgcc ttgataacca ccatgcgctc
     1681 aaggttatcc cgggtctgca ttcgcagtga ttccacacat gtaccaccac ttttccacgc
     1741 cttgtggtat tcctctatca gccagcgtcg ctcgtaatgg ctgacgatac gtcgcgcatc
     1801 ggcggcactc gccactgttg tcgcgatttt tatgctgact tcgctgtacc tgatgaacat
     1861 ccgggcctgg cgggctgccc gccctccttt ttgcatcaca ttcagcgtgt ggcttcccgc
     1921 ggttgccagg acttccggca gttcgaagag cttgccgggt gcttcttcca gccggctatt
     1981 ctgtgcagca cgcaccacga agcgctgtcc gtggctgact ttataatgca ggtaatgcca
     2041 gatatccgct tcccggtcac agacagtgat tacccgtttc tgtatctccc ccagccgttc
     2101 ggccatacgc tccgaagcct gctgccagcg gtaactttct ttttcttcac agggacgttc
     2161 ttttcgctgg tgcttaacac cgtaggtgtc cgtgacccga ctccagcgct gctgttcgat
     2221 aaaaccgact ggcagggcgc tgtcaggggc gtacatcagg acagagtgag ccagcagccc
     2281 gtgcgtcttc gggttagtgg tggtattccc ctggtcatca gatgccgtac tgtggctgaa
     2341 gttaatggtg gtggtgtctt ccagtgcgag gagcagcgga tgagcctcac atgcccttac
     2401 agtggcggta aatccggctt cggcaatggc ttgcggggac acagacgggt tacgtatcag
     2461 gcggtacgca ccttcaacct gagcagtgga ctgggatgat ttcacaatag aaagacctgc
     2521 atgctgagcg agagaagagg tcagtgacac aaggcgtcgt gtacgacgcg gatcaccgag
     2581 acgggcatgt ccagactgct cgttatccca tgaataacaa tcagaaagta ccataacaga
     2641 cccgaagaaa atgaaatata agaaaagatc aacgagtgaa gaaaaagttc aaaaaatggc
     2701 tgccggggag gaaggaaagt accggatgga aagagtcccc cctaaagcag actgacagac
     2761 ataacaaatc cccgggggga tttgtgtata agagacagta cttatctgga ggtaattgca
     2821 atatctctgt gaacttacac agggtgggct taccgcatac actgacactt agcggatcga
     2881 cagaacatta ttaacagagc atcactgaac gctacataat cagagttgca taaataaaat
     2941 gttatgatat aacatcacaa tcacaacatt tgttttcgaa tgtatatgaa tgtaatcaga
     3001 actgtcattt gtacattaat tatacttccg gtgggactac aggcagcgac cagtcattct
     3061 tctatggtta aagatacaat caccattgtc gcgacaggaa atcagaacac ggtatttgaa
     3121 acgccgtcga tggtcagtgt cgtcacgaat gacacaccgt ggagtcagaa tgcggttaca
     3181 tcggccggca tgctgaaagg tgttgccggt ctcagccaga ctggtgcagg acggaccaat
     3241 gggcagacct ttaatttacg cggctatgac aaaagcgggg tacttgttct tgttgacggc
     3301 gttcgccaac tcagtgacat ggcaaaaagc agtggcactt atctggatcc ggcactcgtc
     3361 aaacgtatcg aagttgtccg cgggccaaac tccagtctgt acggcagtgg cgggctggga
     3421 ggtgtagtgg acttcagaac tgccgatgca gcagattttc ttccccccgg agagacaaac
     3481 ggtttaagtc tgtggggaaa tatcgccagt ggtgaccaca gcacaggctc ggggctcacc
     3541 tggtttggta aaactggaaa aacagatgcg ctcctttctg tcattatgcg taaaagaggt
     3601 aatatctatc aaagtgatgg tgagcacgca cctaacaagg aaaaacctgc agccctgttt
     3661 gcgaaaggct ctgtcggtat aacagacagt aacaaagcag gtgccagctt gcgtctctac
     3721 cggaataaca ccactgaacc gggcaattcc actcagacac atggtgacag cggcctgcgt
     3781 gacagaaaaa cagtacaaaa tgacgtacag ttctggtacc agtacgctcc tgtggataac
     3841 agcctcatca atgtaaagtc aacgttatat ctcagtgata tcactatcaa gacaaacggt
     3901 cacaacaaaa cggcagaatg gagaaacaac agaacctccg gtgttaatgt tgtcaacagg
     3961 agtcatactc tgatttttcc gggagcccat cagttaagtt atggcgctga atattaccgt
     4021 cagcagcaga agccagaagg ctctgccaca ctatatccgg aaggaaacat tgactttaca
     4081 tcattgtatt tccaggatga aatgacaatg aaaagctacc cggttaacat tatcgtcggt
     4141 tcccgctatg accggtacaa gagcttcaat ccccgtgccg gagaactgaa agccgaacgc
     4201 ctgtccccaa gggcggcgat ttcagtctca ccgacagact ggctgatgat gtacggctcc
     4261 atatcctctg cattccgagc gcccacaatg gcagaaatgt acagggatga tgtacatttt
     4321 taccgcaagg gtaaacccaa ttactgggtt cctaacctta atctgaaacc agaaaataac
     4381 atcacccgtg agattggcgc aggtattcaa ctggatggcc tgcttacaga caatgaccgg
     4441 ctgcagttaa aaggcggata tttcggaacg gatgccagaa actatattgc cacacgcgtg
     4501 gatatgaaac ggatgcgttc ttattcttat aatgtatccc gggcccgtat ctggggatgg
     4561 gatatgcagg gtaattacca gtctgattat gttgactgga tgctttctta taaccggacg
     4621 gaaagtatgg atgccagcag cagggaatgg ctgggctccg gcaatcctga cacacttatc
     4681 agtgacatca gcatacctgt tggtcataga ggcgtttatg ccggatggcg tgctgaactt
     4741 tcagcatcag ccacgcatgt gaaaaaaggc gatccccatc aggctggtta taccatacat
     4801 tccttttcac tgtcttataa gcctgtaagt gttaaaggct ttgaggcgtc agtaactctg
     4861 gataatgcct tcaacaagct tgccatgaat ggcaaaggtg tgccgctttc aggcagaact
     4921 gtcagtcttt atacccgtta tcagtggtaa cctcgcagga aataaatcat gatgaaaaat
     4981 acaggctata tcttagctct ttgtctgaca gcatcggggc atgtcctggc ccatgatgtc
     5041 tggattacag gtaaacaggc agagaacaac gttaccgcag agattggtta tggtcataat
     5101 ttcccctcaa aggggacaat tcctgacaga agggatttct ttgaaaatcc ccggctttat
     5161 aacgggaaag agacaataac actgaagcca gcgtccacgg attatgtcta taaaactgag
     5221 tctgcaagca aagataatgg ttacgttctg tcaacgtata tgaaaccggg atactggtcg
     5281 agaacctcgt caggatggaa accggtcagc cgggagggca gaaatgatgt ggcttactgt
     5341 gaatttgtca ctaaatatgc aaaatctttt attcctggtg aacagcagat gccagcacaa
     5401 ctctatcagt ctccaacagg gcatgagctt gaaatcattc cgttatccga tataagtcgt
     5461 ttcagtgaaa atgtgaagct gaaagttctg tataaaacgt ccccgctcgc cggagctatc
     5521 atggagcttg actcggtcag ttatctgaca tcatcccgtc atactcatgc agttgagcac
     5581 aaacatcctg ttcataaagc agaactcacc tttgtaacta atgaggatgg tatcgtcaca
     5641 gtaccttctc ttcatatcgg acagtggctg gcgaaagtcc aaaataagaa aagttttcag
     5701 gacaaaagcc tgtgtgatga aactgtcgat gtggcaacct taagcttctc ccgaaattaa
     5761 tgatactatt cctgccctgt attttacggg cagggtatat caaccggaaa tagcctactg
     5821 acatccatgt aaaaaaactc ataattattt tgttgatgta tcttacctaa tacattgata
     5881 gctccagttt ttataccaga gcattctcgt tgtccctatg tacttgtctg caatatcact
     5941 gatatgcagg aataatattc ttggcatgga gtcagttccc cgtcatctta ggtatcactt
     6001 taactcacag gtgtccagta aatatgaaca ggatcccagt aatcattatc aatggtttcc
     6061 ttggcgcagg aaaaaccact cttatgaaaa acctgctcac acaggcaaac aggaatcact
     6121 tagctgtatc tgtcattgtc aatgatatga gcgatctgga tgttgatggt gtacttattg
     6181 caaatacaga aatcgttaat attgcgaaca ataatttcgt gagtatcaca tctgatagta
     6241 tcagcagcca gagtgggatt aagacggtcg acaaggcaat taaaaatatg ctcaggcatt
     6301 atttgcctga cgttatattt gtcgaaacat ccggaggtag ccacccactg ccattaatta
     6361 agtatctgca gactcatgag cgattacagc taaaatcact tttatcactt gtcgataccg
     6421 ttatgctcaa tgatgactat aacggaggga aagcactgat acctgcattt caggagaatc
     6481 ttttccatgg caagcgcaga atagagaatt tactggctga gcaaatcatg ttttgtagca
     6541 ctttgctgtt aaccaaaaag gatcgactgt cattcgacat tgtcaccgat gtggcgaaag
     6601 caatccatcc tctgaatcct tacgtaaatg tgatagctgt gtcatggggg aaccttaaac
     6661 tagcagagtt actaaccctt ccggattacg attttaaccg ggtcggatta cttatcagag
     6721 aactggaaga cacaatcaca gtagaagaag caaaaatttc agcccaaaat ggcgaaataa
     6781 tttcgcgagt gattaaagat gacaggccat tccacccaca aagattatgg gaaacctatc
     6841 attattttat gggaatgggt atttatcgta gtaaaggctt tttctggcta ccaggaagac
     6901 ctgacatggc tctgctctgg aaccaggccg caggaagtat caatctggag tttatcagct
     6961 actggaattc cggtgtattg gcagaccctg ataatcacct gacccacgaa gagcgctctg
     7021 ttttgcagaa aaaagtcaat aaaactatgg ggcggtttgg agacaggaga tgccatttaa
     7081 cgattatagg gcgttctgat gaagtccatg attttacctc cgccttaatt aactgttttc
     7141 tttctgaaga agaaattgtc tggtggcaat caggtggcgt tttcgcggat ccctggcccg
     7201 ttaatatatc ccggctgaac tgatgattaa cgcgatattg caaatatggc atatgaatcg
     7261 atcgtagttt ggcattcgaa tcgatcatga tttgggtaaa cgttcacgga gcgccgtatt
     7321 gacgtttatt tattgggtga gtacgacgtt ccatggcagg agttcatcaa cacggttaga
     7381 aggccattcc ggcaggacgt tgaggatatg gcgcagatac gcttccggat cgataccgtt
     7441 cagtcggcag gcgccgatca gcccgtacag cagcgcacta cgctctccac cgtgatcgtt
     7501 gccgaagaac atgtagatga tatgaccttt atatactcac tatagcattg gtgggatatt
     7561 ccagaggagg tcatcatgta ttcggaaaat attgcggctt acgtcgggct ggatgtgcat
     7621 aaagccccct agcagttgct attgttgcat ctgaacgact tggcaaggtt cgttattatg
     7681 gtatggtacg attaacaacg aagcccagtc tgtacgtcga ttatttcaga aatgcaggga
     7741 ctttacggaa acattctttc ctgctatgaa gctggcccct gtggttttgg gctatatcat
     7801 caactcacag cgatgaatat aaaatgctag gttattgcct cttcccagat agtaaaatca
     7861 ccgatagaca ggatcaaaaa tgatcatcgg gatgcaaccg ctaccgtttg cctcgttctg
     7921 gaaataacct tgctgatggg ggtatggttt ggtacgttgt ggggatttgt atgtggtttt
     7981 cttcatggaa agacagtggt atgcaaccgg gcgttgcatt tgtctcaagc atagtcgcag
     8041 gcatttgctt tggtgttctc atggctgtgt accattagtg tcgaaaggta gttaacaatc
     8101 ttccagactg ggaccgtctg gagtaaagat cgttgtagag gctatatact ttttaaatgg
     8161 actgggggtg agatgggaaa aataaaatat tggctaatag taggatttat tatacttttt
     8221 gcgatttttt acattgctat tagtgacagg gattctacgc tttctaggtt gaaatcagca
     8281 ggtgaaaacg gagatgtaga agctcagtat gctttggggc tcatgtattt gtatggagaa
     8341 attctggatg ttgattatca gcaggcaaag atttggtatg aaaaagccgc tgaccaaaat
     8401 gatccgcgtg cgcaggccaa actcggtgtg atgtatgcaa atggtctcgg ggtaaatcag
     8461 gattatcagc aatcaaaatt atggtatgaa aaggcggctg cgcaaaatga tgttgatgcg
     8521 caatttttgc ttggggagat gtatgacgat ggtctcgggg taagccaaga ctaccagcat
     8581 gcaaagatgt ggtatgaaaa agcggctgct caaaatgatg agcgtgctca ggtcaatctc
     8641 gctgttctat acgcaaaggg taatggtgtt gaacaggatt atcgacaggc caaaagctgg
     8701 tatgaaaagg ctgcagctca aaatagtcct gatgcgcagt tcgctcttgg aattctgtat
     8761 gccaatgcta atggtgtgga gcaggactat cagcaggcaa aagactggta tgagaaagca
     8821 gcagaacaaa atttcgccaa tgctcagttt aatcttggta tgctctatta caaaggtgag
     8881 ggtgttaaac aaaactttcg gcaagccaga gaatggtttg aaaaagccgc atctcaaaat
     8941 cagccgaatg cccaatataa tttaggtcag atttattact acggtcaggg tgtgactcag
     9001 agctatcgac aggcgaaaga ctggtttgaa aaagcggcag agaaaggtca tgtcgatgct
     9061 caatataatc tcggtgtaat atacgaaaat ggtgaaggtg tgagtcagaa ctatcaacag
     9121 gcaaaggctt ggtatgaaaa ggcagcctca caaaatgatg cgcaggcgca gttcgaactt
     9181 ggcgttatga atgaactggg tcagggtgaa agcatagacc tgaaacaagc aagacattac
     9241 tacgagcggt catgtaataa tgggcttaag aaaggttgtg aacggttaaa agagttatta
     9301 tacaaa
//
LOCUS       DADTFG010000176         9125 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.177, whole genome shotgun sequence.
ACCESSION   DADTFG010000176 DADTFG010000000
VERSION     DADTFG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.177"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(65..391)
                     /locus_tag="J1138_004103"
     CDS             complement(65..391)
                     /locus_tag="J1138_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBA8455831.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(399..584)
                     /locus_tag="J1138_004104"
     CDS             complement(399..584)
                     /locus_tag="J1138_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBA8455832.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(581..3220)
                     /locus_tag="J1138_004105"
     CDS             complement(581..3220)
                     /locus_tag="J1138_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBA8455833.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVKLDSACLAKL
                     PQAEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            3428..4417
                     /gene="ldhA"
                     /locus_tag="J1138_004106"
     CDS             3428..4417
                     /gene="ldhA"
                     /locus_tag="J1138_004106"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBA8455834.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4528..4950
                     /gene="hslJ"
                     /locus_tag="J1138_004107"
     CDS             4528..4950
                     /gene="hslJ"
                     /locus_tag="J1138_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBA8455835.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4947..5213)
                     /locus_tag="J1138_004108"
     CDS             complement(4947..5213)
                     /locus_tag="J1138_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBA8455836.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5487..9011
                     /gene="nifJ"
                     /locus_tag="J1138_004109"
     CDS             5487..9011
                     /gene="nifJ"
                     /locus_tag="J1138_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBA8455837.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAASVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPTELLT
                     ALKSDATPEVRRAQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2265 a         2398 c         2241 g         2221 t
ORIGIN      
        1 tgtttccgga aaatagatta atcgtcttct cctgtagcgt tatcccgata ttgcggtttt
       61 gctattaaaa ttttcgcacc catttaccgt ttatcccttg tacatattcc ccgggtttgg
      121 cccgttctac aagcttttgc ccggcgagtt tcgcgatatc atccaccgac acattattct
      181 gctttgccag ttgttgatag ctcgctttac gttcggcatt gatgtcggtt actaatttct
      241 cagtttcagc atctgttttc agcgcaacca gataaccata aaatgtttcc cctacccgcc
      301 cctgagttct ggcttcatcc agagtcaatg ccattacatt gctgcttacc aagccaacaa
      361 gaaatgcaca aagaagtaat gttcttttca tcatcacctc aaaaaagatc gctacgggtc
      421 tcaagcagct cttcgacatc tttgtctgcc ttgatgatga tctcatgctc aattttaacg
      481 ttcatattga tagtgatcgg ttccttaggt gcagcgactt caatgcgagg cgtacagcca
      541 accagcataa aagatgacgt caacgcagcc agtaaaattt tcattgttta tcctcacact
      601 cttttccttg cggacagtca tttcccggta gacgcgcgtt ctgctccagc catgcctgga
      661 gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat attctcttca tgatggtaat
      721 taagatttac cgtaccgctt ttaccatcaa cgcgactggt acctgtaata ttggcctgca
      781 tggttaataa accgagatta tctaaattaa tttttgtcga tgaacggcta atttccatat
      841 agcgcaacca gttaattgct gaacccgcgg tcatattgtc tttcaccacc gcatctgcgg
      901 tgtctttatc cagccgcaag gtcatcggcc cgctatttgc cagccagccg tctttcacta
      961 tccatttttc gttgtttagc cataacggca atgcaccact aaatgccccg gacatggcga
     1021 attgtttcgg attgacggcg ctaaccagtt cgctggagga aagattattc agccgcaaca
     1081 gcgccgggtc atgttgcggc atacgtaatt gtttcatcag cacgttaccg cctaacacat
     1141 caacgctaac atcagtcaac agcaatggtt cttcttcagt ccacggataa cgaccttgca
     1201 aatcagccgt aatatttttc gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa
     1261 cgggaccgcg agtccccaga tgccaggctc catcggcaaa acggaaaggc aggacaaaat
     1321 cgacaccgtt aacctgatta tctggcatcc aggcactacc acccttcaac acgccgtgcc
     1381 ctcccgcgcg gaatccttgt tcaggcgcag ctgaaaatgc aacctgagca tacagttcac
     1441 catcacgtaa gttcatcttc cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt
     1501 gtttaggcca ccaggcgtta ccgcgcagac gaataccgtc ccagcgacca tttacccgaa
     1561 ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag gaaatacgtc ggatcacgcc
     1621 catcgacact aaattttaag gttgatggcg gtaacacact gccgccagtg aacagcgttt
     1681 gcccggcgtc cagtgacagc gcgccgctga atgagggatg ctgtgcgtca cgtccccaga
     1741 cgacgggctt gtcgagaatt aatcgcggct tttctaccgt catcgtaccg tattgtaact
     1801 ggtcgaaacc ggtggagaga tcggtcagcg taatcgtgct gtcatgccac tcaccttttc
     1861 ctgcgacatc ccaggtggca ttcatcggtg taaaactccc ttttccccag tagcgccact
     1921 gccagcggcc agcgtcaggg agaaaatcat tcgccagccc atccatatgt agcacgaaat
     1981 cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt
     2041 gggtgacttt tacacccgct aaaggccagc ggatttcatc gatgtccagc gaatcaatga
     2101 ctcttccctt tgaacgaagt aacgcgccag gctcaaaggt cagcgttggg tcagacagac
     2161 ttccacttag ctgcgcaggt aaacgggcat ataaaatgag gtccgcctgt ttcgcttcac
     2221 cggtcagctg cagaggcagc tggctgttat ccatgcttaa ttttcctggg ccaaaattta
     2281 acaccgcgtt gcctttaccc gcttgcccct gggtcagcac actcagtcgc ccgctgacca
     2341 gagcgttctc gagccctgcc tgccagttgt cgactttaac acccagtcgg ccactcaaag
     2401 gaaaacctgc atacggccag ctccagcgac catcgcttac ggtcaattgt tgacgagtaa
     2461 tttgccacgg caaatcgagc aacggatcgc cgttatcccg tgccagcaca atcaattgcc
     2521 cgctattttc ctgccagtcc agctcggcat ccaccagtga cggttcctgc ggcaagttta
     2581 acgtcgcagt agcatgacca cttaccggaa gtccatccgg cacgagcggc atagtaaatt
     2641 cccccaccag ttttaccggc ggctgatttt caaacgcggc gacatccagt tcgctgactg
     2701 taagttgttg ccctttcagc tggccttgaa atttaacttt ttcgccctga taacgcagtt
     2761 gctggatatc ggaggttaat gcgagagaga gttttccctg ccattcctgc cagggagaaa
     2821 aaatcagttt atcgatattg atccaggtgt taggcagcat ggactgccac tgcgcgaggg
     2881 tttttggagc ggctggcgat tgttccgcct gcggcaattt cgccagacaa gcagaatcaa
     2941 gttttaccgt gccgacgttc aataaccagc ggctgggatg tgaaaggctg gcgttggtga
     3001 tatgcgcaag ctgacaatct cccaccagat aacggagatc ggggatgatt aaacctttac
     3061 gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg
     3121 ccagcgtggg aacccacagc ccgagcgtca tcagcagcgt caacggcaca agaataatca
     3181 gtaataacag cgcgagaacg gctttatatt tacccagcat gggtagttaa tatcctgatt
     3241 tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg tttaaccgtt cagttgaagg
     3301 ttgcgcctac actaagcata gttgttgatg aatttttcaa tatcgccata tctttcaatt
     3361 atatttgaaa ttttgtaaaa tatttttagt agcttaaatg tgattcaaca tcactggaga
     3421 aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt acctgcaaca
     3481 ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga cggaaaaaac
     3541 cgctaaaact gccaatggct gcgaagcggt atgtattttc gtaaacgatg acggcagccg
     3601 cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccttgc gctgtgccgg
     3661 tttcaataac gtcgaccttg acgcggcaaa agaactgggg ctcaaagtag tccgtgttcc
     3721 agcctatgat ccagaggccg ttgctgaaca cgccatcggt atgatgatga cgctgaaccg
     3781 ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac ttctctctgg aaggtctgac
     3841 cggctttact atgtatggca aaacggcagg cgttatcggt accggtaaaa tcggtgtggc
     3901 aatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc cgtatccaag
     3961 tgcggcggcg ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc
     4021 agacgttatc tctctgcact gcccgctgac accggaaaac taccatctgt tgaacgaagc
     4081 cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg gtgcattgat
     4141 tgattctcag gcggcaattg aagcgctgaa aaatcagaaa attggttcgt tgggtatgga
     4201 cgtgtatgag aacgaacgcg atctgttctt tgaagataaa tccaacgacg taattcagga
     4261 tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc atcaggcatt
     4321 cctgacagca gaagcgctga ccagtatttc tcagactacg ctgcaaaact taagcaatct
     4381 ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct tgccgctccc ctgcattcca
     4441 ggggagctta ttcagataat ccccaaagac ctttcatcct ctattcttaa aatagtcctg
     4501 aatcagaaac agtaattgag aaccacaatg aagaaagtag ccgcgcttgt tgcgctaagc
     4561 ctgctgatgg cgggatgtgt aagtagtgac aaaattgctg taacgccaga acagctacag
     4621 catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg
     4681 ccagaaatca gctttggtga aaagatgatg atttccggca gcatgtgtaa ccgctttagc
     4741 ggtgaaggca aactgtctaa tggtgaactg accgccaaag ggctggcaat gacccgtatg
     4801 atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat gctgaaagaa
     4861 ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta
     4921 acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc ggcaagtgac
     4981 tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt accatccagt
     5041 tggcgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc actactggac
     5101 atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac aggttcacta
     5161 ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg catcttttct
     5221 tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca gagggcggcg
     5281 tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat ttttttgccc
     5341 tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga aaatgcagaa
     5401 gaaagccatt tgctaaaatt gaacgattac tactgggcgc gcagcaattt cgtgcgcccc
     5461 tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc gcggttgctt
     5521 cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc tattaccccc agttccacga
     5581 tggcagaaca ggctgatgca tgggccggaa acggcttgaa aaacgtttgg ggagacacac
     5641 cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat ggtgctttgc
     5701 aaactggtgc cctctcaaca tcgtttacgt catcgcaggg tttgctgctg atgatcccaa
     5761 cgctgtacaa actggcaggc gaactgacgc cgtttgtcct gcatgttgcg gcacgtaccg
     5821 ttgccacaca tgcactctct atttttggcg atcattccga cgttatggcg gtgcgccaga
     5881 cgggttgcgc gatgttgtgt gcagcaagcg tccaggaagc gcaagacttt gctctcattt
     5941 cgcacatcgc gacgctgaaa agccgcgtgc catttattca tttctttgat ggtttccgca
     6001 catctcacga aatcaataaa attgtcccgc tggccgatga cacgattctt gaactgatgc
     6061 cgcaggcaga aattgatgct catcgcgccc gggcactcaa cccggaacat ccggtgatcc
     6121 gcggtacgtc cgccaatcct gacacttatt tccagtctcg cgaagccacc aacccatggt
     6181 acaacgcggt ctatgaccat gttgaacagg cgatgaatga tttcgctgcc gcgacaggtc
     6241 gtcagtatca gccgtttgag tattacgggc atccgcaagc ggaacgggtg attatcctga
     6301 tgggctcagc cattggcaca tgtgaagaag tggttgatga attgctgacc cgtggcgaaa
     6361 aagtcggcgt gctgaaagtt cgcctgtacc gccccttctc cgccaaacac ttgctgcaag
     6421 ctctgccggg atccgtgcgc aacgtggcgg tactggacag aaccaaagaa cccggtgccc
     6481 aggcagaacc gctctatctg gatgtgatga ctgcactggc agaagccttt aataatggcg
     6541 agcgtgaaac cctgccccgt gtcattggtg ggcgctatgg tctttcatcc aaagaatttg
     6601 gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct
     6661 ttacggttgg tatttacgat gatgtaacca atctgtcact gccgttgccg gaaaacaccc
     6721 taccaaactc ggcgaaactg gaagccttgt tttatggcct tggtagtgat ggcagcgttt
     6781 ccgcgaccaa aaacaatatc aagattatcg gaaattccac gccgtggtac gcacagggct
     6841 attttgttta cgactccaaa aaggcgggcg gcctgacggt ttctcacctt cgcgtgagcg
     6901 aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc tgccaccagt
     6961 tgcagtttat cgataaatat cagatggctg agcgtttaaa acctggcggc attttcctgc
     7021 tcaacacgcc gtacagcgca gctgaagtgt ggtcgcgctt gccgcaggaa gttcaggccg
     7081 tgctaaacca gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc gcccgcgaat
     7141 gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat ctgacgcaga
     7201 ttctgcctgg cgatagcgcc ctagcagaat tgcagggtgc gattgccaaa agttacagta
     7261 gcaaaggcca ggatctggtg gaacgcaact ggcaggctct ggcgctggca cgtgaatccg
     7321 tagaagaagt tccactgcag ccagttaatc cgcatagcgc caatcgaccg ccagtggttt
     7381 ccgatgccgc tcctgatttc gtgaaaaccg taaccgctgc gatgctcgcc gggcttggcg
     7441 acgccctccc cgtttcggcg ctgccgccag acggcacctg gccgatgggc accacgcgct
     7501 gggaaaaacg caatatcgcc gaagagatcc ccatctggaa agaggaactc tgtacccaat
     7561 gtaaccactg tgtcgccgct tgcccacact cagctattcg cgcaaaagtg gtaccgcctg
     7621 aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc
     7681 gcgggcagaa atatgtcttg caggtggctc cggaagattg caccggttgt aacctgtgcg
     7741 tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc aatatgatgt
     7801 cgcgcctgga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc aacctgccag
     7861 aaatcgaccg tagcaaactg gaacgtattg atattcgtac atcgcagctg attacaccgc
     7921 tgtttgaata ttccggtgct tgctccggtt gtggcgagac gccgtatatt aaattgctga
     7981 ctcagctcta tggcgaccgg atgttgatcg ctaatgccac tggctgttct tcaatatacg
     8041 gcggtaacct gccttcaaca ccatatacca ccgatgccaa tggtcgtgga ccggcatggg
     8101 cgaactcgct gtttgaagat aacgccgaat ttggccttgg tttccgcctg acggtcgatc
     8161 aacaccgtgt ccgcgtgctg cgtctgctcg atcaatttgc cgataaaatc ccgacggaat
     8221 tactgactgc gttgaaatca gacgccacgc cagaggttcg tcgtgcacag gttgcagctt
     8281 tacgccagca actcaacgat gttgccgaag cacatgaact gctgcgtgac gcagatgcac
     8341 tggtggaaaa atcaatctgg ctgattggtg gcgatggctg ggcttacgat atcggctttg
     8401 gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg ctggatacgc
     8461 aatgctattc caacaccggt ggtcaggcat cgaaagcgac accgctgggt gcagtaacta
     8521 agtttggcga gcatggcaaa cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt
     8581 acggtcatgt ttatgtggcg cagatttctc tcggcgcgca gttgaaccag acggtgaaag
     8641 cgattcagga agcggaagca tatccggggc catcgctgat catcgcttat agcccatgtg
     8701 aagagcatgg ttacgatctg gcactcagcc acgatcagat gcgccaactc actgctaccg
     8761 gcttctggcc gttatatcgc ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg
     8821 cgctggattc acgcccgccg tcagaagcac tggaagaaac gttacttcac gagcagcgtt
     8881 tccgtcgtct gaattcgcag cagccagaag tggcagaaca gttatggaaa gatgctgcag
     8941 ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca
     9001 acaccgatta aatgctctgg ataaggatta tccaattcta aaaaaaagcc cggacgactg
     9061 ttcgggcttg tcttcttatg tgttggaaaa tcagtagcaa tgcaatggcc caacagaaaa
     9121 taaaa
//
LOCUS       DADTFG010000177         9076 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.178, whole genome shotgun sequence.
ACCESSION   DADTFG010000177 DADTFG010000000
VERSION     DADTFG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.178"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            219..4466
                     /gene="fdeC"
                     /locus_tag="J1138_004110"
     CDS             219..4466
                     /gene="fdeC"
                     /locus_tag="J1138_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HBA8455838.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVSNSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTMELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEASVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVEDKNGNPVPGTLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSDAQTITFVADKATATVSGIE
                     VIGNYALADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAATSDVHSLVADGKT
                     TATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNKP
                     GTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4591..5448)
                     /locus_tag="J1138_004111"
     CDS             complement(4591..5448)
                     /locus_tag="J1138_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8455839.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5696..6565
                     /locus_tag="J1138_004112"
     CDS             5696..6565
                     /locus_tag="J1138_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA8455840.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6725..7318)
                     /gene="rclC"
                     /locus_tag="J1138_004113"
     CDS             complement(6725..7318)
                     /gene="rclC"
                     /locus_tag="J1138_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBA8455841.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7330..7566)
                     /locus_tag="J1138_004114"
     CDS             complement(7330..7566)
                     /locus_tag="J1138_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA8455842.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(7675..9000)
                     /locus_tag="J1138_004115"
     CDS             complement(7675..9000)
                     /locus_tag="J1138_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBA8455843.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTANNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         2507 a         2043 c         2236 g         2290 t
ORIGIN      
        1 aaatttttac ccataaataa aaatataatc ccgcgcccaa ccacctgatg agtggctaca
       61 gacactggat atattaggtg gcggtgtact ttcttacata aaggtatttc cttttctgtg
      121 gaaaaggaaa ccgggaaatc cccggttttt ctgacaagca gacgccatta tttgtgtctg
      181 cctatgttcg ttaattcgtt catcaggaaa ttatctcaat gtcacgttat aaaacagaca
      241 ataaacagcc acgatttcgt tattcagttc tggcccgctg cgtggcgtgg gcaaatatct
      301 ctgttcaggt tctttttcca ctcgcagtca cctttacgcc agtaatggca gcacgtgcgc
      361 agcatgcggt tcagccacgg ttgagcatgg aaaatactac ggtaactgct gataataacg
      421 tggagaaaaa tgtcgcgtcg cttgccgcta atgccgggac atttttaagc agtcagccag
      481 atagcgatgc gacacgtaac tttattaccg gaatggccac cgctaaagct aaccaggaaa
      541 ttcaggagtg gctcgggaaa tacggtactg cgcgcgtcaa actgaatgtc gataaaaatt
      601 tctcgctgaa ggactcttcg ctggaaatgc tttatccgat ttatgataca ccgacaaata
      661 tgttgttcac tcagggggca atacatcgta ccgacgatcg tactcagtca aatattggtt
      721 ttggctggcg tcatttttca gaaaatgact ggatggcggg ggtgaatact tttatcgatc
      781 atgatttatc ccgtagtcat acccgcattg gtgttggtgc ggaatactgg cgtgattatt
      841 tgaaactgag cgccaatggt tatatccggg cttctggctg gaaaaaatcg ccggatgttg
      901 aggattatca ggaacgcccg gcgaatggct gggatattcg tgctgagggc tatttacctg
      961 cctggccgca gcttggcgca agcctgatgt atgaacagta ttatggcgat gaagtcgggc
     1021 tgtttggtaa agataaacgc cagaaagatc cacatgcgat taccgctgaa gtgaattaca
     1081 cgccagtgcc tcttctgacc ctgagtgccg ggcataagca gggcaagagt ggtgagaatg
     1141 acactcgctt tggcctggaa gttaattatc ggattggcga acctctggaa aaacaactcg
     1201 atacagacag cattcgcgag cgtcgaatgc tggcaggcag ccgctatgac ctggttgagc
     1261 gtaataacaa tatcgttctt gagtatcgca aatctgaagt gatccgtatt gctctgcctg
     1321 agcgtattga aggcaagggc ggccagacgg tttccctggg gcttgtggtc agcaaagcaa
     1381 ctcacggtct gaaaaatgtg caatgggaag cgccgtcttt gctggccgca ggcggaaaaa
     1441 ttacggggca gggcaatcag tggcaagtga cgctcccggc ttatcaggca ggcaaagaca
     1501 attattatgc gatttcagcg attgcctacg ataacaaagg caatgcctcg aaacgtgtgc
     1561 agacagaagt ggttattagc ggagctggta tgagcgccga tcgtacggcg ttaacgcttg
     1621 acggtcagag ccgtattcaa atgcttgcta acggtaatga gcaaaagccg ctggtgctgt
     1681 ctctgcgcga cgccgagggc cagccagtca cgggcatgaa agatcagatc aagactgaac
     1741 taaccttcaa accggctgga aatattgtga ctcgtaccct gaaggccact aaatcacagg
     1801 caaagccaac actgggtgag ttcaccgaaa ctgaagcagg ggtgtatcag tctgtcttta
     1861 ctaccggaac gcagtcaggt gaggcaacga ttactgttag cgttgatgac atgagcaaaa
     1921 ctgtcactgc agaactgcgg gccacgatga tggatgtgtc aaactccacc ctgagtgcta
     1981 acgagccgtc aggtgatgtg gttgctgatg gtcagcaagc ctacacgctg acactgacag
     2041 cggtggactc cgagggtaat ccggtgacgg gagaagccag ccgcctgcga cttgttccgc
     2101 aagacactaa tggtgtaacc gttggtgcca tttcggaaat aaaaccaggg gtttacagcg
     2161 ccacggtttc ttcgacccgt gccggaaacg ttgttgtgcg tgccttcagc gagcagtatc
     2221 agctgggcac attacaacaa acgctgaagt ttgttgccgg gccgcttgat gcagcacatt
     2281 cgtccatcac cctgaatcct gataaaccgg tggttggcgg tacagttacg gcaatctgga
     2341 cggcaaaaga tgctaatgac aaccctgtaa ctggcctcaa tccggatgca ccgtcattat
     2401 cgggcgcagc tgctgctggt tctacggcat caggctggac ggataatggc gacgggacct
     2461 ggactgcgca gatttctctc ggcactacgg cgggtgaatt agacgttatg ccgaagctca
     2521 atgggcagga cgcggcagca aatgcggcaa aagtaaccgt ggtggctgat gcattatctt
     2581 caaaccagtc gaaagtctct gtcgcagaag atcacgtaaa agccggtgaa agcacaaccg
     2641 taacgctggt ggcgaaagat gcgcatggca acgctatcag tggtctttcg ttgtcggcaa
     2701 gtttgacggg gaccgcctct gaaggggcga ccgtttccag ttggaccgaa aaaggtgacg
     2761 gttcctatgt tgctacgtta actacaggcg gaaagacggg cgagcttcgt gtcatgccgc
     2821 tcttcaacgg ccagcctgca gccaccgaag ccgcgcagct gactgttatt gccggagaga
     2881 tgtcatcagc gaactctacg cttgttgcgg acaataaaac tccaacggtt aaaacgacga
     2941 tggaactcac cttcaccgtg aaggatgcgt acgggaatcc ggtcaccggg ctgaagccag
     3001 atgcaccagt gtttagtggt gccgccagca cggggagtga gcgtccttca gcaggaaact
     3061 ggacagagaa aggtaatggg gtctacgtgt cgaccttaac gctgggatct gccgcgggtc
     3121 agttgtctgt gatgccgcga gtgaacggcc aaaatgccgt tgctcagcca ctggtgctga
     3181 atgttgcagg tgacgcatct aaggctgaga ttcgtgatat gacagtgaag gttaataacc
     3241 aactggctaa tggacagtct gctaaccaga taaccctgac cgtcgtggac agctatggta
     3301 acccgttgca ggggcaagaa gttacgctga ctttaccgca gggtgtgacc agcaagacgg
     3361 ggaatacagt aacaaccaat gcggcaggga aagtggacat tgagcttatg tcaacggttg
     3421 caggggaact tgagatcgag gcctcggtga aaaactctca gaagacggtc aaggtgaaat
     3481 tcaaggcgga tttcagtacc ggtcaggcga gcctggaggt agacgccgct gctcaaaaag
     3541 tggcaaacgg caaagatgcc tttacgctga cggcgaccgt tgaggataaa aacggtaacc
     3601 ctgttccagg cactctggtg acctttaatc tgccccgggg tgtcaagccg cttactggcg
     3661 ataatgtctg ggtgaaagcc aacgatgagg ggaaagcaga gttgcaggtg gtttccgtga
     3721 ctgccggaac ctatgagatc acggcgtcgg caggaaatga ccagccttcg gatgcgcaga
     3781 ctataacgtt tgtggccgat aaggcgaccg caaccgtctc cggtattgag gtgattggca
     3841 actatgcact ggcggacggc aaaaccaaac agacgtataa agttacggtg actgatgcca
     3901 ataacaacct gctgaaagat agcgaagtga cgctgactgc cagcccggaa aatttagttc
     3961 tgactcccaa tgggacggcg acaacgaatg agcaagggca ggctattttc accgccacga
     4021 ccactgtcgc agcgacatat acactcacgg cgaaagtgga acaggccgac ggtcaggaat
     4081 cgacgaaaac tgccgaatct aaattcgtcg cggatgataa aaacgcggag ctcgccgcaa
     4141 catctgatgt gcattctctg gtggcggacg ggaagactac tgcaacactg acggttactc
     4201 tgatgtcggg tgtcaacccc gtaggaggaa ccatgtgggt cgacattgag gctccggaag
     4261 gggtgacaga ggcggattat cagttcctgc cgtcgaaaaa tgaccatttc gcgagcggga
     4321 aaatcacgcg tacatttagt accaacaagc caggtacata cacattcaca ttcaactctt
     4381 tgacatatgg agggtatgaa atgaaaccag tgactgtgac aattaacgcc gttcctgcag
     4441 atactgaagg cgctgaggag aaataaaaac gacgatgtaa taaataataa tggcgttgac
     4501 atgcgcagcg ccattattta ctgaatacct accctggtac cgattagacg gtggcagggt
     4561 agggtaaatc ttatgtgatg gatttacagg ctaaaccggg cgaacgggga taaaaatttc
     4621 gcaaacaatg ttattgctat caatttcgac gaagttttca ttgtaataaa atcgctcaat
     4681 atcactgccg ttcactctgg ctaaccccct ggcaggtaat tccatcagat agaggtttcg
     4741 cgcccattca gggtagtgct cccactcacc ttcatagcgg aaagaggcat acagtcctcg
     4801 gttaattgtc cactctttcg acatctctga atcggtttgt ttgcataccg taccggcaaa
     4861 agtttcaact gtgagcaaat cgaccgattt tgttgacggt tcgaacgtgg agacgaaata
     4921 gatatccgtc ttaaaattct tcatacatct gactaaatga tgacgtcgca gtttgacttc
     4981 ttcatccggt aatttattcg acacttcaat atcatattta aatgaattgc caaaaatagg
     5041 tgtctcagga aagttgacta atctacactc cgtcttttgc tgttgacgta ttaaaaaaga
     5101 agggaagata tttgcgagat cccaataatc acggtggcgg tattcacggg gagagcagcc
     5161 gaataacttt ttaaattcgc ggctgaatga ctgctgtgaa tcaaaatgca aagagagtgc
     5221 aatatcaagc atagatttcg cggagagccg gacaagaatg gcggcacgac aaagccttcg
     5281 tttgcgaatg tattctccca aaggcacatg catgaaattt cggaacagaa gctggatgtt
     5341 gcgtctgctg tagccagatt tctgtgcgat atcttcgatt gaaatagggt gctcaagatt
     5401 gcactcaatc cactccagga gctgctgtag aatcttctgc ctgatcatca cattctccgt
     5461 taattctttg tcatatcgca cattttcgtt atgtcctggg ccctaagtgt atcaaaaaca
     5521 gcaaggcgca aggaaaaagc actgatagcg tctgcttcat tgccccaaaa atagagagat
     5581 ttctgccctc cattattaat gaattttttg cataagtgat atcaaaatcc acatactaat
     5641 ttgaggttac gttttaacgt agactcattg ctcataccta atggagggac tgacagtgga
     5701 attttccgta ttaagtaaca atctgaaaat gccgatgatg ggatttggtg tttttcaggt
     5761 taccgataaa gacgtgtgca aacagtcagt gttgaatgct atccgcacgg gttatcgact
     5821 catcgacaca gcagcggtat acggtaatga agatgccgtc ggtgaagccg ttcgtgaggc
     5881 catatctgaa ggtttatgta cccgagaaga gttatttata acgtctaaat tgtgggtgca
     5941 ggatatgttg aatcaggata ttgcagcagc aggtattgaa gcatcattaa aaaaatcggg
     6001 gctagaatac ttcgatctct atttattgca ccaggccatg cgcgattatt tcagtgcgtg
     6061 gcgtgcactc gaagatgcct atgaagaggg caaattaaaa gcgattgggg tttccaattt
     6121 ctaccctcat gttctggcga acttttgtga aacggtaaga gttaaaccga tggtcaacca
     6181 ggtcgagttg catccctatt ttgcccaacc agaggcgctg gcaaccatga agtattataa
     6241 cgtgcagcct gaagcatggg ctccgttagg tggtggacga cataaaccct ttgaaaataa
     6301 tctgcttcag agtattgcag atgcccatca aaaatcaatt tctcaggtca ttctgcgttg
     6361 gaatattcaa cggggagtgg tcgttattcc gaaatcaaca catcaacagc gtatcgaaga
     6421 aaattttgct atctgggatt tctcactgac agagaaagaa atggcgcaaa ttagttcgct
     6481 tgatttgggt tatgttgggg aatcggtaaa acattttaat cctgagtttg ttcgtggttg
     6541 tcttgctgta aaaatacatg attgatatta atcaattatt ttacctgaga cgacaagaat
     6601 cttttaatag gggagtgata ttgaccttca ctctgtcata tctccggtaa tatggcgtca
     6661 ggcttcatca tcagaaaggg agcctgacgc ctgaaaaagt gaacaacaga cagtgttcgg
     6721 attatcaata ttcagttttt aacgttgaac tggattcatt actgcgttgt ttaagaatat
     6781 cccgcgccga atctgccatt atcatgacag cacctgccag catcagagta tctttcaata
     6841 ccagacgacc agcaccggat aaataaggga aaccatgatg agcgtcaccc aatgcgggta
     6901 cccatgcctc cggggtggtg attaaaaatg agagtgttac cagcggcgtg gtaaatgcca
     6961 tcagcccacc caataaacct aaccagcgat tgacaggatt agccaaaacc agcagagcaa
     7021 taatcacctc cacgacgcca agaccgttgg aaaaaccata ggtattatta gccgtttgcc
     7081 atgctcttgc ttctggtttg tattcgcctt cgtgagtcag atgctgttta tagtcttccg
     7141 ggtgttcata aaagaacgac attagtggac tgtttgcgac gaatggtgta atgctgtctg
     7201 cctcgtaagg gacaaacttt aataacccaa tccacataaa aacaatcgca atactcagac
     7261 gaatcaatgt caggccaatt ttatctcccc gacttaacag gtgcaggtat ctttccattg
     7321 taaacatcct tatttataca atactgctgt tgcggttgaa ttattatcaa ttcgcatcga
     7381 tgtaactttc caggatgaag cattatattt tctggccata tcagcaatgg cattctcaag
     7441 aactgtcggt gatgctgata ctgatgagac gctgatatgt ttcagaatta ttttatcatc
     7501 tgcattggtg gaaaatgcca taacgccaga taatagtgtt gcaaataaaa cagatttttt
     7561 aaacatataa aataccatca atgaagtgat taagaagtta taaagtaaca gggaattaat
     7621 aagattcttt tctctgagac gccagaatat ttgttctggc gtctgatttt gagtttattt
     7681 gactaatgaa aatagatcat tgagtgattc gctcatcgac ggatgagtaa atatctgatc
     7741 gcgtaatatg ctataaggca gcccggcatc catcaccatt ttcactatat tgatcatctc
     7801 gtgggagtca acacacagca gtgatgcccc taatatacgt tgggttttat tatcaacaat
     7861 cgcttttaat accccacgag tatcattcat cactctggca cgcggaattg cagctacagg
     7921 caatgtcacc acctgaatat cagcaccact ctctctggcc tgttcttctg tcataccaac
     7981 cctggacagt ggcggtgtca tgaatacgga ataaggcaca tttttccgat catcagtact
     8041 gcgtctgcct tctcccagca actcatcacg cacaatgcgg taatcatcca gtgaaatgta
     8101 agtaaattgc agcccgccgg taacatctcc catcgcccaa atattgtttg cggtggtatg
     8161 taattgcttg tcaacgacaa ttgccccacg ttcgtttact gcgataccgg cattttctgg
     8221 atgtaacgaa gcggtagccg gttgacgacc ggaagctatt aacagtgcat ccaccgccag
     8281 ttgggcgtgc tcgctatgca cttgcacttg attttcatgg tgactgattc gctccacatg
     8341 ggcattgagg attatatcga caccctgatc gcgtaaaatc gtcgcgatat tatcagcaat
     8401 atcccgatct tcccgaggca aaaacagcga agctgcctct aaaatggtta ctttgctgcc
     8461 aaaattagcg aacatagagg cgaactcaac gccaatatat ccgccgccca aaatacctaa
     8521 atgcccaggc aattctttta gattaagtaa tccggtgctg tcatatacac caggcgtggt
     8581 ggtaattcca ggaattggcg gaaccacggc ttgtgcaccg gtattaataa aaattttctc
     8641 gccatgaatc tccagatttc ccccaggccg atgaacacgc aggctatgat tattgataaa
     8701 ctccgcctgg ccgtcgatca cgtcgatatt gggcatatcc gcaagattat gaaaattctt
     8761 attacgtaaa aaattaacca cttcattttt acgctgtatg gcacggacaa aatctgtgtg
     8821 ctgctgtgcg tcatgaacca atgttttggt tgggatacag ccgatattaa tacaggtccc
     8881 gccatacatt gcatttgatt gttcgatgag agccacacgc caacctgctt ttgccagcgt
     8941 gacggctaat gtttttccag ccttgccaaa accaataata actgcctgat atttattcat
     9001 gatgatttcc tgtcgaaacg cgttcatttg acatcatgac tatagacgca aaagagcaaa
     9061 taacatttct tcacaa
//
LOCUS       DADTFG010000178         9044 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.179, whole genome shotgun sequence.
ACCESSION   DADTFG010000178 DADTFG010000000
VERSION     DADTFG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.179"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..842)
                     /gene="gspL"
                     /locus_tag="J1138_004116"
     CDS             complement(<1..842)
                     /gene="gspL"
                     /locus_tag="J1138_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBA8455844.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIAFWQTLKIEDQL
                     QQQQ"
     gene            complement(857..1840)
                     /gene="gspK"
                     /locus_tag="J1138_004117"
     CDS             complement(857..1840)
                     /gene="gspK"
                     /locus_tag="J1138_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBA8455845.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLNTESISDEQSQGWQSHIFFNRKQQSVQIYRRTLQLY"
     gene            complement(1833..2420)
                     /gene="gspJ"
                     /locus_tag="J1138_004118"
     CDS             complement(1833..2420)
                     /gene="gspJ"
                     /locus_tag="J1138_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBA8455846.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            complement(2413..2790)
                     /gene="gspI"
                     /locus_tag="J1138_004119"
     CDS             complement(2413..2790)
                     /gene="gspI"
                     /locus_tag="J1138_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBA8455847.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLEHTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            complement(2787..3296)
                     /gene="gspH"
                     /locus_tag="J1138_004120"
     CDS             complement(2787..3296)
                     /gene="gspH"
                     /locus_tag="J1138_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBA8455848.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            complement(3304..3741)
                     /gene="gspG"
                     /locus_tag="J1138_004121"
     CDS             complement(3304..3741)
                     /gene="gspG"
                     /locus_tag="J1138_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBA8455849.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            complement(3751..4947)
                     /gene="gspF"
                     /locus_tag="J1138_004122"
     CDS             complement(3751..4947)
                     /gene="gspF"
                     /locus_tag="J1138_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBA8455850.1"
                     /translation="MNYRYRAMTLDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNNRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            complement(4944..6425)
                     /gene="gspE"
                     /locus_tag="J1138_004123"
     CDS             complement(4944..6425)
                     /gene="gspE"
                     /locus_tag="J1138_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBA8455851.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVIRGDTSWDEVMRVASATLENEA"
     gene            complement(6435..8387)
                     /gene="gspD"
                     /locus_tag="J1138_004124"
     CDS             complement(6435..8387)
                     /gene="gspD"
                     /locus_tag="J1138_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBA8455852.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            complement(8371..>9044)
                     /gene="gspC"
                     /locus_tag="J1138_004125"
     CDS             complement(8371..>9044)
                     /gene="gspC"
                     /locus_tag="J1138_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBA8455853.1"
                     /translation="INQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIA
                     LNGIVLTSNDETSFVLINEGNEQKRYSLNEALESVPGTFIRKINKTSVVFETHGHYKK
                     VTLHPGLPDIIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQP
                     GDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQR
                     NEGTQ"
BASE COUNT         2129 a         2199 c         2340 g         2376 t
ORIGIN      
        1 tgctgttgtt gctgaagttg atcttcaatc ttaagcgttt gccagaacgc gatgccccgg
       61 ctgccaacaa aagaaagaat ggcgagaacg agacagctta ctgccaggcg ggcgagccat
      121 tttcccgatt tgcttacagt tttctgttta cgaaaaacac catgcagcat gtcatagcgc
      181 tggaatgcaa tatcggcaga atacaaactc agtgatggta tttcaggatg ctggataagt
      241 ggattggccg ctgcaacgcc gtgaggcgct acaccataac aacgcatatt ctctggcgga
      301 aactggttag caagatgctg taaccagtgt tcatccagtt cgttgaaggc atgtgcggca
      361 ctgcgaataa gccagcttgt ttgctggtta accagagtcc agctatctac ttcccaggga
      421 agatagcagc catctggcaa aacccgcgtc acgttgagtc cggcagtatg taaacgctca
      481 agataacgac tgagtttctc cgactgaatg cctatcacct cgacactttc gttttgttta
      541 tcgacaaccg tccagtgcca attttggctg gcgtcgggta atgtctcctc cgccaaccac
      601 tgtaatgttt gcgcggtgag tttgtatttg gcgttgggca gcgtgaggga acgatataaa
      661 agaccctcag gtgggatcaa aaggtgaaca tttccggcac cgggctgatc ggccagcgac
      721 tccagcggaa gatcgtctgt gagcgtatgt accgaagata cgctatctgc ggagaacgtc
      781 atccattccc aatgcttacg cagcgtcgag aaagaacgta ttaccatcaa cgattcaggc
      841 atcacgatcc ttatgattag tagagttgca gcgtacgccg atagatttgg acgctctgct
      901 gtttacggtt gaaaaaaata tggctttgcc atccctggga ttgttcgtcg ctgatacttt
      961 ccgtattcag cgtaaaatag cggctatagg gaaagagatg ccctttcacc tgagcaacca
     1021 ggggcttcac gccggagaaa tcctgctgcg cccagtaaag aaatgcatct gtggttagcc
     1081 agccttccct gggccttttt tgtaataaga cccgagcgtc agcatccgtg atgttattga
     1141 ggaataatgc tctgaagagc ggaatatcgt tctccgttag catattaaga ttgatgctaa
     1201 gttccgaggt cgggagaacg cagacatacg ggataagccg ctgatagatg ttttcggtta
     1261 tgccttttat ctgacgcaat tcaccggtca gaaaaagcat ttgattggcg ctatggcgag
     1321 gcggcgtttg gctttggtaa aagttatctt ctgcgccgtg aaagcgtggc gaatcatcgg
     1381 catcaatata gtcagcgatt gattgcacga tctcatcggt gtttccgcga tcgataccgg
     1441 cgttaatcag cagggcactg aatacctggg cggggaaatc aggggaagcc agcggggcgt
     1501 cagagatttt tgctaaggca ttgagattaa aacagtgctg ggcatcgcgt aactgccact
     1561 tcactgtatt gccattttcc agctgcaact gttgcggttg tgcccagtac tgctgcaacg
     1621 tggtttgtct gtcgttatct ttaacgtcct gggtgaggct ggcaagcgca agtttctccg
     1681 ccagctcaat atcatattgc cgttgcaaat gatggtttac ctggcgggtt cgatgaagct
     1741 ggccgtgaaa actgatcgtc atgtctgcag ccagagcagc catgagcgcc agtaacatga
     1801 gtacaatgag cagtgctacg ccgcgttgtt cattattcat ggttattatt cccggcctgc
     1861 gtctgagctg gcgactcttc tctcgctaac tgctcaggta atgcaaaacg gcgctgtaac
     1921 gccccatatt gctgagtctt cagatgtaac gtgactgaga gaggaagctc ctggctttct
     1981 ccgctgttac tttccagaag aaagctctcg acatgttcca gcatcgcctg ggcgggttga
     2041 tccttcctgc catccacaga agtacgaaca gcacggtaga gcgtatgatt tcgcagatac
     2101 cagtgaacag tgagtaaggt ttgtgcttca ctgagagggg ccagcggatc gcgactttgc
     2161 gtggtaaaaa tcgcttcttc acccaacacc atgattttgt ccgtactccg attccgccgg
     2221 gcgaccagtt gcaggagatc gttatcaaga atggtgatgg tgcgctgtag ctgattgaac
     2281 tgctggattt ctttctgtga acgttgatgc aactcggaag cttgagaaaa aatcataaac
     2341 gccagcacgc taagcattga gaaaatcgcc agcgccgcca tgacttccag caatgtgaaa
     2401 ccctgctggc gattaatcat cttgtgcctg cccggattta ttattgatgc tttgatagcg
     2461 cgtaagcgag gttgtctgac cgctgggtaa tgtcaccgtt atggtgtgtt ccagaagcgt
     2521 gccgtctttg cttgagtgga tatcgctgcg ccagttccac tcttcgccat ttattaactc
     2581 tttgccagaa ctggacgtgt tttcctcgtc gaatgaatct tgtgactgaa gctggttatc
     2641 cgctatccac aatgccagcg tctcattacg catccgctcg atggcagttc gttgcccttg
     2701 catactgctc attaaagtca gcgcaacagc ggtgaaaata ctcatcgcca gtaagacttc
     2761 aagcagtgtc atccctgatt gtttgttcat gggcgggtat ctctggcgag cgtttgatcc
     2821 aggggccatg agcctgaact gactaacgtc aggagcggct caccggtccc ggcattagcc
     2881 atcaacaaag agaagggcgt gatttgtccg tcggccagaa tcaccacctg cggttggttg
     2941 ctgtcatcag gtttaaatgg ctgaagttga attgagagtt cttcacccca gtcatttttg
     3001 ctctcgtctg cggcatcttc ttgtaatgga acccagcgcc aggctgatgg tgttttgccc
     3061 ggtaccataa tgcgccatgc tgaatcagag aaatggatac cgacaggctg accactgagc
     3121 gtggcgcggt cgatggctaa ttcaagtgca gcggtaaacc gcgccgctgt ttcgcgcgca
     3181 cgagtgctgg cggcatcctc ccgaccatat gtgaagagca ccacacttgc cgtgatagcg
     3241 accagcgcca gcaccagcat catctccagc agggtaaacc cgcgttgctg attcatcgct
     3301 gacttacttt ttcttcttgc tcaaacccca gttggtgatg tcgtcctcgg ttcccatttc
     3361 accatcgggt cctgctgaaa gcagatcgta tgcaccatgt tcaccaggat taacgaggac
     3421 ataatcattg ccccagggat cggcaggcag acgcttgata taaccttcct tgttatagtt
     3481 tgcggccagc ggtggcagtg tcggcgcttc tactaaggat tcaagcccct gatttgtggt
     3541 tggatagcga tgattgtcga gcttgtacat atccagggcg ttttccagcg ccacaatatc
     3601 gctgacggct ttttgtttat ccgccttttc tttattgccc atcaggttgg gaaccaccag
     3661 gctggcgagt acgccaatga tgacaattac caccataatt tccagtaatg taaaaccgcg
     3721 ttgcttatcc gttgcgcgca tatgatttcc ttaattaatc attgagttaa gttgaagaag
     3781 aggttggagt accgacacga caataaacag gacgatcagt gccatcgtaa taatgagtgc
     3841 tggctcgaag atggagagcg ttaaggcgat ccgattttgt tggagtgtct cctggttatc
     3901 tgcggctctg accattaacg tgccgagttg cccgcttttt tcgccagagg ccaccatgta
     3961 gagcatcatc ggcgggaaaa ttgcggtttg ttccagtgaa agatgaatgc tgttaccctg
     4021 gcgaacgttt tctgccgcat ttgccaggcg ctggcgaatt tcgaggttgt tgaggctttc
     4081 ggtggacaaa ttcatcccat ccagcagagg gacgccgctg gattgcaaaa tacttaaagt
     4141 gcggagatag cgtgcgctgt taatggcgca aatcagcggg ccgatgagcg caacgcgcag
     4201 taacatggca tgaaaacggt ggcggttatt gccgcgtttt aaccagagcc agaaacccac
     4261 agcgacaata aatactgtcg ctaataatgt cgggccggta cgttgcaacg tgtcgctcag
     4321 acctaaaaga atgcgtgtac tcagtggcag ttgctgcttc atatgcacga actgttcggt
     4381 aattttgggc acgacagcag tgaggagaat aatcacgacc acaatcgcca ccgtagtgag
     4441 catgcagggg tagatcagtg actgaatgag cttgctgcgg attttctgcc ggttttcatt
     4501 gtaatcagcc agcttttcca acaccggggc cagcagaccg ctcttttcgc ccgcttttac
     4561 cagggtacgg tagagcgaat cgaaaagcgt gggaaaatgt tgtaacgcat cggaaagggg
     4621 atgcccttca aggatggcgc tgcgtacctg atttaacacg tcagccagtc gattattact
     4681 gctttgttga ccaattacgg caaggctctc ttccaggggt aatgccgctg cgcttaaggt
     4741 tgccaactgc cgggtgaaaa gcgtcagttc actatggctg atcctcgggc gacgtgtttt
     4801 tactcccgaa cttttttggg ggcgaatatc caacaggaaa agcccttctt cacgcagccg
     4861 cagtcgtgcc tgacgttcat cgttagcatc aatgatcccc tgcaattttt gaccatccag
     4921 ggtcatggcg cgatagcgat aattcatgct tcgttctcca gcgtggcact ggcgacgcgc
     4981 ataacctcat cccaggaggt gtcaccgcgt atcacttttt gcaggccatt tttgattaag
     5041 gcattgtgtt gttgccggac cagtcgctcc agtgcttgtt catccacatt ttcatgaata
     5101 gcggcccgca attccggcgt caccaccatc atttcgtgga tcgccatgcg cccctggtag
     5161 ccggattgat ggcaatgagg gcagcctacg ggagtgccaa ttgtcgtcac cgcgagctga
     5221 tgatatttaa acatctgcgc ttgctggggc gatacgggcg tgaattgtcg gcattgcgga
     5281 cacagacgac gaaccagacg ttgcgcgata atccctgcca gtgaagacga aagcaggaat
     5341 gattcgacgc ccatgtcgcg tagccgggtg actgcgcctg atgcactgtt agtgtggagt
     5401 gttgagagta ccagatggcc tgtgagcgag gcctgaaccg caatttgcgc ggtttctgta
     5461 tcacgaatct cccccaccat gacgacatcc gggtcctggc gaagtatggc gcgcaggccg
     5521 cgagcgaaag acatatccac gcgggtattc acctgcgttt gtcctatgcc ttccagttca
     5581 tattccacgg gatcttctac cgtcagaata ttacggccag gggtattaag cgccgaaaga
     5641 atggcgtaga gcgtggtgct tttaccggag cctgtcggcc ctgttaccag gataataccg
     5701 tgcggaagct gaatgagatt ttctaaatcc tgtttatccg ctgctgtcat ccccaggttg
     5761 ttcaatgaaa gctggaggct gtttttatcc agcaggcgga gcacagcgcg ttcaccatag
     5821 atggatggca gtgtggatac gcggacatct atgttacgtc gcccgatacg caaactgatt
     5881 cttccatcct gtggaatacg tttttcggcg atatcaagac gagccatgac cttaattcgg
     5941 gagataagca gtgccgccag ttttttgttg ggctgtaaaa ttgtccgcaa aacgccgtcg
     6001 atgcgaaaac ggatcgacat tgttttttca taggtttcaa tgtggatatc ggaggcggtt
     6061 tctttgatgg cctcactcaa aatagcattg atcaagcgga taaccggtgc cgctgaatct
     6121 tcattcagga gatcttcatt tgcgggcatc tcttccgaaa gcgagagaag atcgacggat
     6181 tgatcgatat cctgcgcaat ctgttgcgac tcaccggtat tctgatggaa caccgcttcc
     6241 agtcgggctt caaaatcagc ctgtgacaag ctggtcatgg tctggcactg gccaacttga
     6301 cgttgaatat cgagtaaccg ttcgaatggc gtatcggcga gataaataat ctcgccctct
     6361 gaatagagat aaccaattcg ttgtgccagc gcccaactgg cggggtacgg tgagtgaatt
     6421 ctcataagaa tgcctcaccg tgacgatggc gcaggggcgt gactgttgaa cgtgttctca
     6481 ttcagcaccg gcaaatcttc cgagccaacc agcgcttttg atttcccgtc gattcgcagc
     6541 tgttgctcct gacggtagcg ggtgtatttc tcttttgaca gtgagcgata aacatcatcg
     6601 tcacggataa tcgtcgggcg gataaatacc atcaggttgc gtttagcgcg ctcggtcgag
     6661 gtatagcgga agagttgtcc cactaaagga atatcgccaa gcagaggaac ctttgacact
     6721 tgctctttag aaaaatcatc cagcaatccg cccaggacca ccgtttcacc ggttttaacc
     6781 agcacggcgt tttgaatagt acgggtgtta aatgtcgggc cgagcgtcga attagacgaa
     6841 gagtcaacgc tggaaacctc ctgctctatt tcgagcagta ccgcgtcgcc ttcattgacc
     6901 tgcggagtga ctttgagttt tgtgcccacc gttttgcgtt cgacagtatt aaagacgtta
     6961 tccccagaag tggtctgaga gccggatagc accggcacat cctgaccaac gttgaaggac
     7021 gcgagtttat tatccagcgt tacgatgctc ggggtggcga ggatgtcatt tttattgtta
     7081 ctggccagcg cagtcaacaa tacgccccag tcgccattga agaagcctgc agccatgcca
     7141 ttgtaggcgc taaacatatc ccaggcagga ttcgcgctgg tgatcccgcc attcttttta
     7201 taatcagcta caccttgcgc agcgttaaaa accggtaatc ccgtattggt aaattgctgt
     7261 gcgccaacgt ttttattcgc ccattgcacg ccgaggttga gtccatttcc atcctgaact
     7321 tcgacgatga ttgcctcaac cagcacctgt gcacggcgaa tatccagacg cgcaattacc
     7381 gtggcgagtt tctcctggac ggactggtca gcggtaatga ccaacgagtt ggtctgttca
     7441 tcggcggtaa tggccacatt atccatcgca gaagtagaag aaggcttacg cgaattccct
     7501 ttttcatctt tcagcttttc ggaaacaccg gttagcactt ccaccagatt cgtggcttta
     7561 gcatatttca ggtaatagac ccgggtattt ccttcctcgc tctcttctac atcaaggctt
     7621 ttcagtaatg aagttatgcg ctggcgtgct ttttccggcc cgctgatgat aagagagttg
     7681 gttcgcttat ccgccacaat cttcgcggaa aggagggcag gcatctggct tttaccgtgg
     7741 ctttcgctga ttaattgatt aagaatctcg gcgagatctt ccgctgatgc atattccaga
     7801 tgaataattt gctgcttctc tgtgccgatg acatcaacgc gctttatgac ttcaatcagt
     7861 ttattaatgg tggaggcacg accggtcaga ataagaacgt tggagggttc ataatgcaca
     7921 acattaccga cgctacccgc atccatcatc tggcggagca ggggagctag gtcacgagca
     7981 ggaacgttct caagcggtac gattcgggtg accaactcat cacctacgcc tggacgagaa
     8041 ctgtcagcaa tcatccctgg cgatgttttt acattagctg agcgcaccac tttcagaaaa
     8101 ccattgtcca gtgtgatcac ggaataaccg taaagatcaa gaatacttaa aaagaactgg
     8161 tagtactcct gttggctaaa cgtatcatta ctgcgtacgg aaatggttcc ctgtaccgaa
     8221 gggtcgatca ggatcgtttt gccaagatgc tgacccacta tttccacgaa ctggcggata
     8281 tcggcgttat tgaagttagc gccgtattgt tcgttctcag cgtgtcctgc acaaggcatg
     8341 agtagtgctg ccagcaagca gcaggtgatt ttattgagtc ccttcattgc gctgtccatt
     8401 cattcctgta agttccgcga cggaaacatt tatcaagcgg ggtacgccgt tgcgacgaat
     8461 tgtaaactga gcactttgtt gagttagcag taaacttaat gcctgtgaaa cctcatcagg
     8521 gtgggtcagc gaaagattat tgatcctgag ggcgatatcc ccaggttgca gcaagctggt
     8581 ggtaaatgca ttgagaccct tacgaggatt caatcgaaga ccgtatatct gttctccatc
     8641 acgtataggt gtggcgataa tataatcggc gagtacattt tgattttccg aatcaggctg
     8701 cttgatgatg tcaggtagcc ctggatggag agttactttt ttgtaatgcc cgtgagtttc
     8761 aaacacaacg ctggttttat ttatttttct aataaacgtt cccggcactg actcaagggc
     8821 ttcgttgagg gagtatcgct tttgttcgtt cccctcatta attaagacaa aagaagtctc
     8881 atcattcgat gtgagtacaa tgccattaag cgctatagag agcttaggtg tttcctgatt
     8941 aactgccacg ggatttgcat cttttacccc agcatgattt tcgttgcgcc ataaagcggc
     9001 aaggctgaat tcagactgaa atgcatttgt tggttgatta atga
//
LOCUS       DADTFG010000179         8676 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.180, whole genome shotgun sequence.
ACCESSION   DADTFG010000179 DADTFG010000000
VERSION     DADTFG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.180"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(59..919)
                     /locus_tag="J1138_004126"
     CDS             complement(59..919)
                     /locus_tag="J1138_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBA8455854.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPFGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(916..1695)
                     /locus_tag="J1138_004127"
     CDS             complement(916..1695)
                     /locus_tag="J1138_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBA8455855.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWLAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLVQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMATGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(1673..3082)
                     /locus_tag="J1138_004128"
     CDS             complement(1673..3082)
                     /locus_tag="J1138_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8455856.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     RFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPADSFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHTVSV"
     gene            complement(3104..4558)
                     /locus_tag="J1138_004129"
     CDS             complement(3104..4558)
                     /locus_tag="J1138_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBA8455857.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGVLCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPYADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWTLLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(4628..5413)
                     /locus_tag="J1138_004130"
     CDS             complement(4628..5413)
                     /locus_tag="J1138_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA8455858.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATCSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            5731..7008
                     /locus_tag="J1138_004131"
     CDS             5731..7008
                     /locus_tag="J1138_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8455859.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVTMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            7035..8513
                     /locus_tag="J1138_004132"
     CDS             7035..8513
                     /locus_tag="J1138_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA8455860.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAELPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPTRRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         2173 a         2283 c         2112 g         2108 t
ORIGIN      
        1 cttatcaggc ctacaagtcc ccagcccaga accatcggca ggatcatccg gcagcacgtt
       61 attggcaatc tgcgtgaatg tttgtcacca gtgcctccag caccactttc cagtcatcaa
      121 ccacgccaac atctgcctgt gaaaataccg ctgcactggc gtcatgatta atcgccacca
      181 caaacttgct attgcgtacg cccgccatta acgctgctgc gccggaagcg ccaacaacaa
      241 tacacacctc aggtgccagc agatgtccgg aaataccaat cactttttcg gcatcaacgc
      301 caccgttcat cactcgtgcc cgactgtagc ccacttcggc tcccatcttt tctgccagca
      361 tagcgatttc ctggttatct gcctctccgc cctgccccac caccagcacc cgtctggcct
      421 cagctaatgg gtcacgggta acatttttca ggttttcgac gctgataagc cagtctggaa
      481 gcgcgccggg gacaatgttc agttgctgca tcccaaaggg caacgtggcg tttttatctg
      541 ctcccgcctg acgtgccagc gaaaggcata gcgggcgttt ttctgtttgc agcgtcgccg
      601 tcaacgcatt tccccagtgg gatttgcgca cgcttacggt tggaatatct aacgaagtca
      661 cctggcagat gcttgcacca tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa
      721 acgcgcccgg aggaaaaagg acaacctccg caggcgttcg ttgccactgt tccaccagcg
      781 catccagcac ctgttccgca accatcggct gtggttctat ttgccaatgc gttagtgtgc
      841 tgccgctgaa atcctgcgcc gccagccagc ccgccattgc ggcatgttcc tgatttattg
      901 tcacaattgc gatattcatg gctgcatcct ttggcgtaaa tagtcctgcc atagcttttg
      961 cgctttttct gctaccgttt gcccatcaat cagcgccgct ctgcgtcgtt gctctggcct
     1021 tgccagttgc aggcactgta tcgctggcgc ttccgcggca acagtttcgc gggtaatctc
     1081 cgctttcccc gtcgccattc gctggcgcat tcccggtact ggcaaggcca cttcaccaca
     1141 ctgacgcacg gcaatcaccg caggcaagcg aacccggcaa cagcgcagtc catgttcagt
     1201 acgctgttcg agggtgataa acggcgcgtc gagagtaaaa cgctccacct gggtgaagca
     1261 gggccaaacc agcatttccg ccaacaaaaa tggcgtttgc ccattctgcc cttcgctgct
     1321 ttggcagcca gtaattatca gatccagcgg attctggtac tgccattcgg caatatggcg
     1381 agcgacaaat tccggcgcaa agcgcagatc tgccgccgtc tccagcaata cagcttcctc
     1441 aaacccaagt gccatgagat agcgcagcca gtgcaacgcc cgttcatccc ccatgctcaa
     1501 tgcggttaaa gacatcggcg taccgttttt tctctgcacc aacagcagcg cggcagcggc
     1561 ctgttcatca gcaccgagta aacttcgcag tagcgaaaca tccggtccgc tattaccctg
     1621 agccgccgcc agccactctt tttccgccag catcccggca tccggttcgg ctttaaacgc
     1681 taacagtatg ttcattcgca cctcctacat ttcccgccgc caccagcggg agtgctttgg
     1741 tttctggtgc ccatagccag gtcacaacaa aaccaaccaa caacactgcc gccaggagca
     1801 ataaggtggc ttgcattccc cactgtgcca gcacccacgg cagcaggcca gtacttaccg
     1861 ccgcccccaa ccgactcatg gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag
     1921 gaaaactatc cgcaggcaaa atgcccacca gattactgac tgccgaaatg gtggtgctga
     1981 agagaacaaa aagcagcagc gttaatgaac tgccggaagg caaacaggcc atcactacca
     2041 gcgttgccgc cagcagcaaa aaacttccca gtaaaaatct gcgatgcgct aacaagtggg
     2101 tcaacactaa ccctaataac gcgccaacaa ttaacaacgc attaagcatc aggctggcag
     2161 tcagcgcatc ttccagacca atagtttggg cgatggtcgg cagccaggta taaatcacaa
     2221 accatgggat tacgaggcag acaaagaaga cgctgttaaa cgccgtgcgc cgccagtagc
     2281 gcgaagagaa caaggttttg atgtgtttat gggtcgccgt tgccacttca tcgcccagta
     2341 aaacatgcgg gccaaaatag cgatgaacga tagcgtgagc ttctgcaaaa cgcccctggc
     2401 gcagtagcca gcgtggcgat tccggcgtgc cccaacgtag cagcgtaatc agcaaggctg
     2461 gtaacgccgc tgatgccagc agccagcgcc aggcctccgg gctttcggaa ataaagtgat
     2521 gtccggcaat acttgccagc acatagccta cggtccacac cacactgaat gcgcccagca
     2581 aaataccgcg atggcggcgc ggggaaaatt cagccagcaa ggtgtgaccg actgagtaat
     2641 cgcctcccag cccaatgccg atcaaaatgc gcaatccaat aagatgctct ggcgtggtgg
     2701 caaaaaactg taaaaacgaa gcaagcgtaa tcagcataaa actgaaggtg aagatttttt
     2761 gccgcccgat atggtcggag atccacccca aaaccaggct gccaagaaac aaaccaagta
     2821 acgccgagcc gccgatcatt cccgccataa acggcgtcag ttgcatggcg ggcgtaagct
     2881 gaataatggc gtaaccaata acgccgagga cataaccgtc ggtcaggtgt gcgccgaaag
     2941 tgagcgcagc aattcgacag tgaaaacggt taagcggtaa atcatccatt cgcaccggtg
     3001 aagtgttcat ctttacccct tcattgtccg gtaataagaa cggcttctcg cctgagaagc
     3061 cgaaaaggca acgccccgtc atcgcggaac gctgcctgac acattatttt tcaatcggat
     3121 agatagtacc cgtgttcata atgccattag gatcgaactg ttttttcaga ccttccagca
     3181 acgtccacgc gctgccgtgt tccagtttgc tccagtgaac gcgatgttta ccaataccgt
     3241 ggtggtgcac catcgaaccg ccgaggcgaa tggtttcttc gcaaatgatt ttgttgagcg
     3301 gattgtggta cttgtcgatt tcctcttccg gcttacagtc aacgacgttg tagtcgtaga
     3361 cgaagtacat gttggtgccg ttctggtagc tgtgagagga gtgaccgccc agcatggtga
     3421 tgtcatcggc gtacgggaac tcggtacgaa tacggttaat aacgctttcg tagatttcgt
     3481 gaatgcagct ccagcagccg gacacttcag tggtaaagcc catgttgccg gttttgagga
     3541 tctgcacacg ttcggcagcc actttatccg gtccccagtt caggttgtta aaccaggttt
     3601 cgatcagctt gctgtccacg cgctggcatt gcgggtaacg cgccacgatt tcagcaatcc
     3661 cttcgcccgt cgccttcgca atgcgagggt taccttcagc cataaagatc agcacgcatt
     3721 tgccgtcggc aaaatgggtg aagtgttggg tgccatcttc agcatcatac aaacgagcaa
     3781 tcgacggacg atacccttcc accatgattt cacgcaggat gttgaagccg gttttcatgt
     3841 cttccagcac atagccatag aagagattgt tttccggcgt aaatttgaag attttcactg
     3901 tgacttcagt gatatagcac agtacacctt cgttgccgat gatgatatga cgaatgtccg
     3961 ggccagccgc gcgacgtggc acgtttttaa tgcgtgtgac ggtgccatct gccagcaccg
     4021 cttccagacc aacgaccata tcctcgattg cgccgtagag tgtggaaaac tgcccgatac
     4081 tgcgggtcgc caccaggccg cccatctgcg ccagcggctt tgactgcgga gaatgccccg
     4141 tggtgtaacc tttttcacgc aacgcgtttt ccagcacctc cagcggaacg ccacattgcg
     4201 ccgtggcctg catattctca atatcaatat taatgatttg attcatggcg gagccgtcga
     4261 gcaccaccga gttttccaca acggtttcca gcccaccttc ggtggcggaa gcaccggtac
     4321 gtggcacacc gttaattttg tgcgcattca taaaattcag cacacgggat acttgttctg
     4381 tagaaccgag ttttacgacc gctgccggaa tcggcaaagt ataaattcca tgaatatccg
     4441 gaaatttacg aaaacggtca atactgtttt tctttaatac cgtttcatcg gtaattacgc
     4501 gatctgcacc aacaatttcc tttagctggt cgacaatcgc tacgcgagat aaagacatag
     4561 taattccttc ctgataataa aaatggaata gttattaata agagaaaaca gaaaccaccg
     4621 ttatgaatta gcgcactaaa taaccgccat caaccactaa taaatgtccg ttgacataat
     4681 tcgatgccgg acttgcgagg aatacggctg cacccattaa atcctgagta tcaccccaac
     4741 ggtttgccgg aatatgatca agaacacgct ggttagtttc tggattacta caggtcgcca
     4801 gcgtaatatc ggttgcataa taaccaggag caataccatt cacctgaata ttatattggc
     4861 ctaattcatc gcaataggct ttggtgaatc cggcaagagc atgtttagtg gcagaataag
     4921 caggtgacca ttgaccacct aagtaagaga acaatgaaca gatattaatg attttaccgc
     4981 ttttctgcgg gatcataatt tttgccgctt cgtagcttaa ctcgaatgcg gcggtcaggt
     5041 tcacgtcaat catcgggtcc cagtcggcac gaccaaagtc cagcacctta ttcagcttac
     5101 aaataccggc attgttaacc agaatatcta cggtaccgaa gcgctcacag caggcagcga
     5161 taatcttctg cggcgcgcct tctgcggtga tatccacctg cataaaatca acttcgacac
     5221 cctgtttttc aatcatttcc tttgtttcgc cgttatcttt gacgaaacta ggaataaaga
     5281 catttgcgcc agctttggcc aacgccatgg caaatgcctg gcctaaaccg ctattcccgc
     5341 cggtaacaat tgctgtttta cctttcaggg agaaaaaatc cattgagaac gcattgagag
     5401 attcgattga catagttagc tccaatttcc gtaaggcaaa aaaaaagaaa ggtaacttcc
     5461 cccgcgagag ggaagttacc tttctcatta tctctggtaa ctggagataa tttttagcat
     5521 aaggacataa ctgctaatgt gatcgtaatc acagtgtgat gttcgtattg tcaaataatt
     5581 taaaaaagca gaagcgtgat tactctcaca caacacctct gctttccatg ttatttctta
     5641 ttgcaattac tagagacctg agaaaagtaa tccacctgaa agggcggctt acttttctct
     5701 ttttttgtct attacaaatg ggaatacgat atgcaacaca actcatatcg ccgttggata
     5761 accctcgcga taattagttt tagcggcggc gttagtttcg acctggctta tttacgttat
     5821 atttatcaaa ttcccatggc aaaatttatg ggattcagca ataccgagat aggtttaata
     5881 atgagtacct ttggtattgc ggctattatt ctttatgccc ccagcggcgt tattgccgat
     5941 aaattttcac atcgcaaaat gattacttcc gcgatgatca ttaccggatt actgggtctg
     6001 ttaatggcaa catatccacc gctgtgggta atgctctgta ttcaggtcgc ctttgcgata
     6061 acgacgattt taatgctgtg gtcggtgtcg attaaagccg catcgttgct gggcgatcat
     6121 agcgagcaag ggaaaattat gggctggatg gaagggctgc gcggcgtcgg tgtaatgtcg
     6181 ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac cggatgacag cgccagcctg
     6241 aaaacggtca ttattatcta tagtgtggtt tacattctgc tgggcatttt gtgttggttt
     6301 tttgttagcg ataacaacaa tctgcgcagt gccaataacg aagaaaaaca gtcattccag
     6361 cttagcgaca tcctggccgt tttgcgcatc agcaccacct ggtattgcag catggtgatt
     6421 tttggtgtct tcaccatcta cgccattctg agttactcca ccaactatct gaccgaaatg
     6481 tacggcatgt cactggtggc ggcgagctac atggggattg tgatcaacaa aatcttccgc
     6541 gcgttatgcg gcccacttgg cggcattatc accacctaca gcaaagtgaa atcccctacc
     6601 cgcgtggtcc aaatcctttc catcatcggt ttgctggcat tgacagccct gctcgtcacg
     6661 aactctaacc cgcaatcggt cactatgggg attggcctga ttttattact gggattcacc
     6721 tgttacgcct cacgcgggct gtattgggcc tgccctggcg aagcgagaac accgtcttac
     6781 attatgggca ccacggtagg tatttgttcg gtgattggat ttctgccgga tgtcttcgtt
     6841 tacccgatta tcggctactg gcaagacacc ctgcccgctg cagaagccta ccgcaacatg
     6901 tggctaatgg gcatggcggc gcttggcatg gtgatcatct ttaccttttt gctgttccaa
     6961 aaaattcgta ctgctgatag cgcccccgca atggctagca gcaagtaagc caggaaaatt
     7021 acaggagaaa aatcatgtcg aaaaaataca tcatagggat tgatggcgga agtcagagca
     7081 caaaagtggt gatgtacgat ctggaaggta atgtggtttg cgaaggcaaa ggcttattgc
     7141 agccgatgca cacgccagat gccgataccg cagaacatcc tgacgacgat ttatgggcat
     7201 cattatgctt tgccggccac gatttgatga gccagtttgc cgggaataaa gaagatattg
     7261 tcggcattgg cctgggctca atccgttgct gccgtgcgct attgaaagcc gatggtacgc
     7321 cagccgcgcc gttgattagc tggcaggatg cacgcgttac acgtccttac gagcacacca
     7381 atcctgacgt ggcctatgtc acctcttttt cgggttatct gacacatcgc ttaaccggcg
     7441 agtttaaaga caatatcgcc aactattttg gtcagtggcc agtggattat aagagctggg
     7501 catggagcga agatgccgcg gtaatggaaa agtttaatat cccccgccag atgctttttg
     7561 acgtgcaaat gccgggcacc attcttgggc atatcacacc acaagctgcg ctggcgacac
     7621 atttcccggc tgagctgccg gttgtttgta ccaccagtga taaaccggta gaagctctgg
     7681 gagccgggtt actggatgat gaaacggcgg taatttctct tggcacctat atcgcgttga
     7741 tgatgaacgg taaagcactg ccgaaagatc cggtcgcgta ctggccgatt atgtcttcta
     7801 ttccgcaaac attgctgtat gaaggttacg gtatccgcaa aggcatgtgg acagtgagct
     7861 ggctgcgcga catgttaggc gagtcgttaa ttcaggatgc caaagcacag gatctttcac
     7921 cggaagattt gctcaacaaa aaagcgtctt gcgtgccacc tggttgtaat gggctgatga
     7981 cggtgctgga ctggctgacc aatccgtggg aaccgtacaa acgcggaatt atgatcggct
     8041 ttgattccag tatggattac gcatggatat atcgttcgat actggaaagc gtggcgctga
     8101 cgctgaagaa taattacgac aatatgtgtc atgaaatgaa tcactttgca aagcatgtga
     8161 tcattactgg cggcggttcg aacagcgatc tgtttatgca gatttttgcc gacgtgttca
     8221 accttccgac acgccgaaac gccattaacg gttgtgcaag tctgggggct gcgatcaata
     8281 cggcggtagg tctggggcta tacccggatt acacaacggc tgtcgataaa atggttcgcg
     8341 tgaaagatat cttcataccg attgagagca atgccaaacg ctacgacgcg atgaataaag
     8401 gcattttcaa agacctaacc aaacatactg atgtgatcct gaaaaaatcg tatgaagtga
     8461 tgcatgggga attggggaat gcggattcga tccagagctg gtcgaatgcg taagtgagga
     8521 aggccgggcg ggaaactgtc cggcctgaac atacctgaat ggttatcccc gctgacgcgg
     8581 ggaactcata tccggttttt gctggtgggc cgcgccgttc ggtttatccc cgctggtgcg
     8641 gggaactctc taaacataac ctattattaa ttaatt
//
LOCUS       DADTFG010000180         8534 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.181, whole genome shotgun sequence.
ACCESSION   DADTFG010000180 DADTFG010000000
VERSION     DADTFG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.181"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(187..1689)
                     /locus_tag="J1138_004133"
                     /pseudo
     CDS             complement(187..1689)
                     /locus_tag="J1138_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(1871..2611)
                     /locus_tag="J1138_004134"
     CDS             complement(1871..2611)
                     /locus_tag="J1138_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HBA8455861.1"
                     /translation="MNKVFVVSVVAAACVFAVNAGAEEGKSGFYLTGKAGTSVVSLSD
                     QRFLSGDEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWISAGIGYARIHQKTTG
                     ISTWDYEYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGMTYNF"
     gene            complement(2829..3197)
                     /locus_tag="J1138_004135"
     CDS             complement(2829..3197)
                     /locus_tag="J1138_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455862.1"
                     /translation="MSAQEKTVQIDSFDAKFLDINTLSRLIYERKSFVNENVSDVSET
                     VKRVEHEIEKTKLSCRVYTEYRSTALAGSLWSPTVILGVASAVAIGVHNLSTWNPDYE
                     IGKNYIKRRLSVKYKKAEVL"
     gene            3931..4310
                     /locus_tag="J1138_004136"
                     /pseudo
     CDS             3931..4310
                     /locus_tag="J1138_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232055.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
     gene            complement(4506..4808)
                     /locus_tag="J1138_004137"
     CDS             complement(4506..4808)
                     /locus_tag="J1138_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBA8455863.1"
                     /translation="MVLLTAGHLIPDRYVLVDAHQQPVDFTQGEYGLLLALAQNSRRV
                     LICEQLLDLIHGETTEVFDRTIYVLIMRLCRKIEINPHWCFASPIQRLASFWSNLS"
     gene            5106..5447
                     /locus_tag="J1138_004138"
     CDS             5106..5447
                     /locus_tag="J1138_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455864.1"
                     /translation="MNLKNIILSTVLSIASCHALAVGNSQIALSNLHVENTPSGTKAI
                     VGYAENKSNSVVKNAYVKFNLLKDGVVIGQTIDTASNLEPGQKWKIQAFINTFNGSPD
                     SFKVTEVKVVN"
     gene            complement(5782..6364)
                     /locus_tag="J1138_004139"
                     /pseudo
     CDS             complement(5782..6364)
                     /locus_tag="J1138_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6465..6677
                     /locus_tag="J1138_004140"
                     /pseudo
     CDS             6465..6677
                     /locus_tag="J1138_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7001..7717)
                     /locus_tag="J1138_004141"
     CDS             complement(7001..7717)
                     /locus_tag="J1138_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455865.1"
                     /translation="MNSNALLKNSSLFVAYMGCLGWGSAYFYGWGVSFYYGFPWWVVS
                     AGIDDVARSLFHAITIMVILFLSWGAGVLFFLGIKNKASMHELSFFRLFLASFLLFVP
                     VVIEFSVLKNHLALKLLTLSVAVSLILVFLIRTCGHRVSASCFSESVFVKKHISEICF
                     VGFVIYFWVFSFSVGFYKPQFKKEYEMMNYNDGWYYVLARYDTTLVLSKSFKSGNGRF
                     LVIGSEQLKDYEFNMVRVNL"
     gene            complement(8210..8501)
                     /locus_tag="J1138_004142"
                     /pseudo
     CDS             complement(8210..8501)
                     /locus_tag="J1138_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
BASE COUNT         2515 a         1970 c         1787 g         2262 t
ORIGIN      
        1 tttcataatg ctttattgtc aaacacgtta tggctgacat caaaaaaacc ggatttcctc
       61 tgccagcggg taatcacctc cccggtgttt tcggttggtc tggttactcc tgtctggtta
      121 ttagcaagat aattgctata aacagtggaa aactcatcgt acataatctg gtgatgaaca
      181 ttacgcttat tttcccttga ccggaagaat cagaggctgt ggtttcagac agtctgccag
      241 tacattcctc tctccgttaa aaaccataac gggttcatta tcttcgtctg ttagcggatt
      301 gaatggcggt atattttcag tacgaatgcc ggtcagccac tgaaaaatac ctgcgaaatg
      361 acgggcactg atttttctgc tgacggactg atgagacgtg atgtcactgg cggtaataat
      421 caggggaacg ctgtagcctc cttgtatatg gccatcatga tgaacaggat tagcactatc
      481 gctgaccgac atcccatggt cagaaaagta aagcatggcg aaatgacggg aatgccggcg
      541 aaggatacca tcaagctgac cgagaaagtt atccagttta ctgatgctgg cgaggtaaca
      601 ggcaaccttt cggggatact gctccaggta atgattcggc taggagtgaa gccggtcaca
      661 cgggttcgga tgagacccca tcatgtgcag gaatatcacc ttcggagagg atttatccgc
      721 cagtgcacgt tctgtttcct gtaacaacaa catgtcatcc gttttacggg aagcaaagct
      781 gcctttcttg aggaaaacgg tatgctccgc atcagaagca ataacagaga tgcgtgtatc
      841 atgctccccc agctttccct gattggatat ccaccatgtg ctgtatcctg cttttgctgc
      901 cagcgccacc acgttgttgc cggagtcagg gttctgctca tagtcataaa tcagtgtccg
      961 gctcagggaa ggtacggtac tggctgctgc cgatgtatag ccgtcaataa ataaaccggg
     1021 agctgtattc agccacggcg tggttggcac gggatagcca tataccgaca tataatccct
     1081 gcgcacactc tcaccagtga caataacaat tgtgtcatat aacggtgttc ccggcaggat
     1141 tttccagttg tcagccccgt gctgactcaa ttgtttataa tgttgcattt cacgcaatgt
     1201 gtcagttgtc cccacaacag ttcctttaac catccgcaac ggccagctgt ttactgagca
     1261 taatacgaac agcagcagtg ccagccagtt acggtgacca cggcggtgtg ttcgccagaa
     1321 aatcaccatg aatacctgaa tcgcggcact gaccagaaaa tgataaacag gaatcatccc
     1381 ggtaaactcc gctgcctcat cagttgtggt ctgcaacaac gcgacaataa aactgttgtt
     1441 gattttaccg tacgtcatac cggcaggcgc atacagtgca caacagaaca gaaataacag
     1501 cgctgtaatg gatgtgaggg tatttctgtg tgcaaggagc agaaggagaa acagaagcag
     1561 cacatttcct gttgcattct tctcagtgta tccgcatgca attgtggtta ttgcagaaac
     1621 aacaaaaaag aataaaaaca ataaaatccg gggggtgttg cccggacaaa acagttttct
     1681 gatattcatc ggagtatatc gacaacatta ttatgaagag aacaagataa taaaaatcag
     1741 aaattatctg taaaacagat aacagacaac cctgcagtaa tagactgcag ggtatttctg
     1801 tttaattgca gaagatatgc aattatctta attacagaat atacgcaatt atcgttcagg
     1861 agcagtgtcg tcagaagtta taagtcatac caagcatgat gtcatgactt ttaacatcaa
     1921 cttctgactt atatttatcg ccccactcgt ccttgtaact cacactgctg tcacctgcat
     1981 caagatagcg atagctgagg tccagagcga tatccggggt tacgtcatag cggacacccg
     2041 cgccaaggct ccatgcgaag ttgtcagcag agcctgaacg tgacaacgat tcgcgaccac
     2101 tgcttccgta ctcataatcc caggtactga taccggttgt tttctggtga attctggcgt
     2161 agccaatccc tgcagatatc catggtgtga atgcgctgtc attccggaag tcatagtacg
     2221 cattcagcat tagtgtgttg actgacacct cattcttcag gtcatcacgc cagtaaccac
     2281 ctgaccaact gtctttatct acgttatact tcgaatcagc ttttccacga gcgtaaaact
     2341 ccagttctgt acgaaccgga atactgaact gcggataaaa atcataacca gccgcgatac
     2401 caccactgaa taccgtatca tggtcatcgc cgcctttata ctttgacgtt tcttcctcat
     2461 ctcctgacag gaaacgctgg tctgaaagtg acaccacaga ggtaccggct ttaccggtca
     2521 gataaaaacc gcttttacct tcctcagcac cagcatttac tgcaaataca caggctgcgg
     2581 ccaccactga aacaacaaaa accttattca tttcaattat ctccgttatt gtatcatcat
     2641 tttaatatat atttttaata tttaaaaacc gggctaaagg cagcctcaac actccacact
     2701 acggaagcca gacttttact ctacacaaaa taaatatatc ttctcagtga ggacgtgaac
     2761 gcctgtctga aattatgtga atacaacctc cccagaatac aaacaggcag gagcactgga
     2821 gtaaccgatc acaatacttc tgccttttta tattttaccg ataatctccg tttaatatag
     2881 ttcttaccga tttcataatc ggggttccat gtagataaat tatgaacacc tattgctact
     2941 gcactcgcca ctcccagaat tacggttggt gaccataaag aaccagctaa agctgtactc
     3001 ctgtactcag tatatacacg acaacttaat ttcgttttct ctatttcatg ctccactctt
     3061 tttaccgttt cactaacatc ggaaacattt tcgttcacaa aactcttacg ttcataaata
     3121 agcctgctta atgtatttat atctaaaaat ttcgcatcaa aagaatcaat ctgtactgtt
     3181 ttttcttgtg ctgacatctt cttttcctgg ctggtatggc aagtgacgga gacaagagaa
     3241 acgttttaag ctcagttatc tccgccatca tttcccacga atgacaagta attttgccta
     3301 ttttaaaacc atgcaaaagg cagggtaaaa ggagaaaatt cgatcgaata gatcgacaaa
     3361 atcgatcata catgatgaag attttttatc gaatccataa aaatagtgac agctaaccgg
     3421 cgttgcagga acagtcagaa atgggcgttt gggaaagagc catagcacta cgtcgtcgct
     3481 gacatagagg aactgtgctt tgttgataag atcctttata cggcaaccaa tccactggac
     3541 aaaagatgaa ctacgtaatc accgggttct cactgacgaa atacagaagt taatgacaca
     3601 actgtgccat gtaccttgta caacagcggt ggaaagctct cagaacaatg gaattgcaga
     3661 aaggtgttaa aacgatgaaa gccttcatac ccaaatcgaa tgtaagaacg gcagtaaaga
     3721 ctgaattgcg taaccttgca gtagctcgag tattacactg catagtgtgc agggttatct
     3781 cccatcgaga aaatatcggc gccagcgaat aacgtcacct tagatgtagc agttgccaaa
     3841 tagtgactca agggcgggct taccgcatac actgacactt agcggatcga cagaatatta
     3901 ttagcagatc atcactgaac gctacgtaat tatcgtaata aaggcttttt ctggcaacca
     3961 ggaagacctg acatggctct gctctggaac caggccgcag gaagcatcaa tctggagttt
     4021 atcagctact ggaattccgg tgtattggca gcccctgata atcacctgcc ccacgaagag
     4081 cgctctgctt tgcagaaatt ctggggcggt ttggagacag gagatgccat ttaacgatta
     4141 taggacgttc tgatgaagtc catgatttta cctccgcctt aattaactgt tttctttctg
     4201 aagaaaaaat tgtctggtgg caatcaggtg gcattttccc ggatccctgg cccgctaata
     4261 tatcccggct gaacagacga ttaacgcgat attgcaaata tggcatctga atcgatcatg
     4321 atttggcggc atccatctcc gttcacagtt tggcatgctt ccgatcattg attggcatcc
     4381 agccagtcac tccgggaatt aactcttttc accgcagtct ccattgccgt gtttaaacca
     4441 atggagacgt caatgtccaa aaagagaata tccaggagca ctatggatac ctgttttaag
     4501 atccttcagc tcaagttcga ccagaagctg gctaaccgtt gtatcggact tgcaaaacac
     4561 caatggggat tgatctctat tttgcgacac agacgcatta tcaatacata gatggtgcga
     4621 tcaaatacct cagtggtctc accgtggatc aaatccagca attgctcaca gattaagact
     4681 cgtcgggagt tttgagccaa cgccagcagt aacccatatt caccttgagt gaaatctaca
     4741 ggctgttgat gagcatcaac cagcacgtaa cggtccggga tcaagtgtcc agccgttaaa
     4801 agaaccactc tactaccctg ctcgacctaa gcctcggcgt tcagccgcct gaacgggtat
     4861 ggcaagggtg aaaagaaaca gcatccccac agtaccgacc agacgacagg atgatgctgg
     4921 aacagaaagc attcgcacct cccttagaat tagacagtgc gtacaggata cgtaagacag
     4981 ggtgacgggg cggcgataaa ctctatttac aaagctgaaa attttctgac gatgaaaaac
     5041 tattcaacaa ggttatctga ggcgttaaaa taaccagctc gattaacgac taacttgagg
     5101 tgaatatgaa tttaaaaaat ataattttaa gtactgtttt atcaatcgct agttgtcatg
     5161 ccctggctgt aggtaattct caaatagcgc tatctaacct tcatgtggaa aacaccccaa
     5221 gtgggacgaa ggcaattgtg ggttatgctg aaaataaatc aaactctgtt gtcaaaaatg
     5281 cttatgtaaa atttaatctt ttaaaagatg gagtcgttat cggtcaaaca atcgacacag
     5341 catccaatct tgaaccaggc cagaagtgga aaatacaagc cttcattaac acctttaacg
     5401 gcagccctga ctcattcaaa gtcactgaag tgaaagtagt taactgagtg tgccactgac
     5461 tgccgctaca aaaattatat ggaggtcctg ggaaagggca gctctgcacc aggccgccat
     5521 aaaggaatca aatactctcg ttgtttatga caccgaagaa cttttatgtg agattaccca
     5581 gcggccccga gtttcccctg aagtcggtga gttgcagttt caccgtgcgg atcgcgtagt
     5641 ggacatttcg cacaagtcga aaaccatata agagcgtatg aaaatcactc tcctcggccc
     5701 catcgccatc agcatggccc gctgaacaat acaatcccgc aatgcagggc tccccagtgg
     5761 tcgctgtttg ctgttgcttt tagggatagc aacatgttca tgaccaccac gtttataccg
     5821 gtgagtcggc tgctgacagc tgaccacccc cagctctttc atgagcctgc cagcaagcca
     5881 gcgtcccatc tggtagcctc tccgggttgc cattgtggcg atgcttcttg ctccggccga
     5941 accgtggctg atgccatgta gctcaagtac ctgactgtgt aatacagccc atctggtttt
     6001 tcaggacggt ttttccagta tctgtagcta ctgcgatgaa ccctgaacac atggcagagt
     6061 gtgaccaccg gataatgcgc tctgagtttc ccgattatcg ggaactattc agggagtctg
     6121 atatcaagag cgcggtagcc ttttttaata tcgtattttg ttcctccaga cgacgcacct
     6181 gcttttccag ctcacgaata cgttgctgct ctgatgtgag tggtactgca gaaggcgtga
     6241 tatcctgacg ttcccgtctg agctgacata cccatgcctc cagagtagag aaaccaacat
     6301 tcatagcttc agtagcttcc cggtatgagt aacctttatc aacgataagt tgggcgcatt
     6361 ccagattaaa ctcaggagag aaggtacgct ttgttttctt gttgttttat gttgtggtga
     6421 gagtatcacc tttaatcagg tggccaaatt tactgtgcca ctacaacgcg atggaggaga
     6481 gcatcaatgg tctttacaaa acggaggtaa tacaccgtaa gagctggaaa aaccggacag
     6541 aagtgaaact ggccacacta acgtgggtgg actggtataa caatcgacga ttactgaaaa
     6601 ggctgggcca tattcctccg acagaaacag aaaaagctta ttatgcctcc atcggaaaca
     6661 atgatctggc agcctgagtt cacagataaa atactctcca ggaaaaccgg gatggttcac
     6721 cggagtgtaa aagtgtggct taaaattatg cagttgccgc ctggcggtca agactgtcgc
     6781 tacaggataa atcgctccgg tgaaacacaa ctgcgcaaat caccatactg tgcatacgta
     6841 ttaccgtgaa ggagatcggt gagttacggg gttattgggt gatctccttc catgaaaata
     6901 cgcagagcac ttaaatcgct gctggcgcgc actaaaaacg tgtcaaccta gttccattag
     6961 cgtaacatca aagcgagtat ttaagaaaca ggagagcaac ctacaggtta acccgaacca
     7021 tattaaattc ataatctttt aactgctcag acccgatcac cagaaatctg ccattacctg
     7081 acttaaatga ttttgataaa acaagagtgg tatcatatct ggctaaaaca taataccacc
     7141 cgtcattata attcatcatt tcatattctt tcttgaattg cggcttgtaa aagccaacag
     7201 aaaatgaaaa aacccagaaa tatatgacaa agccgacaaa acatatttca gaaatatgtt
     7261 tctttacaaa gacactctct gaaaaacatg atgctgaaac acggtggcca caggttctta
     7321 taagaaaaac aagaataagg gataccgcta ctgataaggt aagaagtttt aatgccagat
     7381 gattctttag cacagagaac tcaatcacca ccggaacaaa caataagaat gaggcaagaa
     7441 aaagtctgaa aaagctcaat tcatgcatgc ttgctttgtt ttttattcca agaaagaaaa
     7501 gtactcccgc gccccatgag agaaacagga tgaccattat agtaattgca tgaaacaggc
     7561 ttcttgcaac atcgtcaata cctgcactga caacccacca tgggaaacca taataaaaag
     7621 agacacccca cccgtaaaaa tatgcacttc cccatccgag acatcccata taagccacaa
     7681 ataaggaaga atttttgagc aacgcattgc tgttcatttt tacccctgta aaactggaat
     7741 aagtaacaga attgcattga cattgtcaag atgaacagca tgcattgtaa cagtctgcag
     7801 tttatgttct ccgtggataa ttattcttat ttcatggtga tttttgttat attttaaaat
     7861 gcatatctca ccatttagtt ttttatatca caaaaacata gtgatgcaat catacacacc
     7921 tgcctctatc gtacagagaa aacgggcaat tatttgtatt attccttcat aacgacttca
     7981 gtatacagga tgaccgcaac cacgctcctt ctcctgcccg ggcgaagtgc ctggttcaga
     8041 caattacatt aactggctga aattttcgac gcggtacagg tctccctgct ttgaaaggtg
     8101 gcctggcgct gcaatttttt acttttgttt ttgtatccgg aaaaacacat ctgcaagaat
     8161 cattattctc ttgcctgaag ctatccagca tcctcagata tttcgcactt tatgagctgg
     8221 catcagaaaa atgtgccagt aattttttat taatgacttc aaacagttcc tgttcatcat
     8281 gggtcatgat aatcccagat ttctttcaga tacatgcttt gtgcaagagt gagcctctga
     8341 ataaaaaaca taccttcaga tgtcagactg atttttttag cacggcgatc ctccctgcta
     8401 tccgtccttt ttacatatcc ggctttttct aaaaaatctg taatgcgggt aatattagtt
     8461 ctggtaaact gaagtttctg gcttatctct gaaggtgaca gacaatggtt ctccgctgcg
     8521 aataatgtgg tgag
//
LOCUS       DADTFG010000181         8514 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.182, whole genome shotgun sequence.
ACCESSION   DADTFG010000181 DADTFG010000000
VERSION     DADTFG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.182"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(115..855)
                     /locus_tag="J1138_004143"
     CDS             complement(115..855)
                     /locus_tag="J1138_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA8455866.1"
                     /translation="MSGAVNSILEVKSGRTEYILSPVFEPVWNQTGKIFAFEMLSDIR
                     SAKDGRKICASVFFRSAPPDIQYKILISQLKTAETLHKWCLQKKIMLSVNISRVVALY
                     LRKHGIPGRPGTHIRLEVSEDFPAVALKPGDDPLLRFLSERFTLWLDDFGSGNAGLMW
                     GLSGMFERVKISHEFFHYALKNRSAMPLLHAVADTVACHNRGVILEGVENEALFRIAR
                     DMNVQGCQGWLYRRVGADELSAITEQYG"
     gene            complement(1159..2058)
                     /locus_tag="J1138_004144"
     CDS             complement(1159..2058)
                     /locus_tag="J1138_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148673348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8455867.1"
                     /translation="MAYSQPSFALLCRNNQTGQTFQSGDSSFNITLSPTVQYDKAITV
                     LDLNQLVLCQNEDAFGQNYDYLRVRQGTGFSPSLDAKTYGRLDFTNRLSGYSQTLPLQ
                     QDTRPTEAYWQYGVWKPFPAKMYLYPEPGVFGKLIHAGELVATVYVNKFSTMGQEAGE
                     RNFTWRFYATNDVYIQTGTCRVSSNNVKVDLPSYPGGPVTVPLTLRCDQTQSVSYTLS
                     GSVTGSGNTVFANTATSGAGGVGVQLSDNAGLVPAGQPRSLGQVGSSPVSLGLKASYA
                     LTGQASPTPGAVQSVINVTFSYN"
     gene            complement(2120..2623)
                     /gene="focG"
                     /locus_tag="J1138_004145"
     CDS             complement(2120..2623)
                     /gene="focG"
                     /locus_tag="J1138_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151062262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1C fimbria minor subunit FocG"
                     /protein_id="HBA8455868.1"
                     /translation="MKLKAIILATGLINCIAFSAQAVDTTITVTGRVLPRTCTIGNGG
                     NPNATVVLDNAYTSDLIAANSTSQWKNFSLTLTNCQNVNNVTATFGGTAENTNYYRNT
                     GDATNIMVELQEQGNGNTPLKVGSTKVVTVSNGQATFNLKVRAVSKGNAGAGSINSQI
                     TVTYTYA"
     gene            complement(2645..3172)
                     /gene="sfaG"
                     /locus_tag="J1138_004146"
     CDS             complement(2645..3172)
                     /gene="sfaG"
                     /locus_tag="J1138_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial adhesin minor pilin SfaG/FocF"
                     /protein_id="HBA8455869.1"
                     /translation="MVKDIIKTVTFSCMLAGSMFVTCHVCAVGSVVNITGNVQDNTCD
                     VDINSRNFDVSLGSYDSRQFTAAGDTTPASVFHVGLTSCGSAVRAVKLTFTGTPDNQE
                     AGLIQINSINGARGVGIQLLDKDKHELKINVPTTIALMPGTQTIAFYARLKATYLPVK
                     AGNVDAVVNFVLDYQ"
     gene            complement(3185..5815)
                     /gene="sfaF"
                     /locus_tag="J1138_004147"
                     /gene_synonym="focD"
     CDS             complement(3185..5815)
                     /gene="sfaF"
                     /locus_tag="J1138_004147"
                     /gene_synonym="focD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial biogenesis usher protein
                     SfaF/FocD"
                     /protein_id="HBA8455870.1"
                     /translation="MFSGDGGQLLSDKSLTGSAGGGNNRMKFNILPLAFFIGIIVSPA
                     RAELYFNPRFLSDDPDAVADLSAFTQGQELPPGVYRVDIYLNDTYISTRDVQFQMSQD
                     GKQLAPCLSPEHMSAMGVNRYAVPGMERLPADTCTSLNSMIQGATFRFDVGQQRLYLT
                     VPQIYMSNQARGYIAPEYWDNGITAALLNYDFSGNRVRDSYGGTSDYAYLNLKTGLNI
                     GSWRLRDNTSWSYSAGKGYSQNNWQHINTWLERDIVPLRSRLTMGDSYTRGDIFDGVN
                     FRGIQLASDDNMVPDSQRGYAPTIHGISRGTSRISIRQNGYEIYQSTLPPGPFEINDI
                     YPAGSGGDLQVTLQEADGSVQRFNVPWSSVPVLQREGHLKYALSAGEFRSGGHQQDNP
                     RFAEGTLKYGLPAGWTVYGGAWIAERYRAFNLGVGKNMGWLGAVSLDATRANARLPDE
                     SRHDGQSYRFLYNKSLTETGTNIQLIGYRYSTRGYFSFADTAWKKMSGYSVLTQDGVI
                     QIQPKYTDYYNLAYNKRGRVQVSISQQTGKSSTLYLSGSHQSYWGTDRTDRQLNAGFN
                     SSVNDISWSLNYSLSRNAWQHETDRILSFDVSIPFSHWMRSDSTSAWRNASARYSQTL
                     EAHGQAASTAGLYGTLLGDNNLGYSIQSGYTRGGYEGSSKTGYASLNYRGGYGNASAG
                     YSHSGGYRQLYYGLSGGILAHANGLTLSQPLGDTLILVRAPGASDTRIENQTGVSTDW
                     RGYAVLPYATDYRENRVALDTNTLADNVDIENTVVSVVPTHGAVVRADYKTRVGVKVL
                     MTLMRNGKAVPFGSVVTARNGGSSIAGENGQVYLSGMPLSGQVSVKWGSQTTDQCTAD
                     YKLPKESAGQILSHVTVSCR"
     gene            complement(5885..6580)
                     /locus_tag="J1138_004148"
     CDS             complement(5885..6580)
                     /locus_tag="J1138_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial biogenesis chaperone SfaE/FocC"
                     /protein_id="HBA8455871.1"
                     /translation="MMKHMRIWAVLASFLVFFYIPQSYAGVALGATRVIYPEGQKQVQ
                     LAVTNNDDKSSYLIQSWIENAEGKKDARFVITPPLFSMQGKKENTLRIIDATNGQMPE
                     DRESLFWVNVKAIPAMDKAKTGENYLQFAIVSRIKLLYRPQGLVIPPEQAPGKLEFTR
                     ENGGLTLLNPTPYYLTVTDLKAGNKSLENTMVPPQGKVTVNIPGGYTGGDITYKTIND
                     YGALTEQVRGVVK"
     gene            complement(6621..7145)
                     /gene="sfaD"
                     /locus_tag="J1138_004149"
     CDS             complement(6621..7145)
                     /gene="sfaD"
                     /locus_tag="J1138_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial minor subunit SfaD"
                     /protein_id="HBA8455872.1"
                     /translation="MAVMLFLLSPAALAGNHWHVMLPGGNMRFQGKIIAEACSLALSD
                     RQMTVDMGQLSSNRFHAAGEYGDSVGFDIHLQDCSTVVSQGVGISFYGVSDIHEPELL
                     SVDEENDASDGIAIALFNESGELVKLNQPPENWVHLTRGDMKLHMQARYKATHYPVTG
                     GKANGQVWFSLTYL"
     gene            complement(7231..7773)
                     /locus_tag="J1138_004150"
     CDS             complement(7231..7773)
                     /locus_tag="J1138_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8455873.1"
                     /translation="MKLKFISMAVFSALTLGVATSASAAIVNGGTVHFKGEVVEAACA
                     VNMNSADQTVQLGQVRAAKLDADGKMSSPVGFDIELNDCSSAASSGASIIFSGPQGGQ
                     ADILSLQGSSAGSATNVGIKVMDYKGAAVKFDGVTVSSEYTITDGTIKIPFQARYVAV
                     GGAAGAGIANADATFKVQYQ"
     gene            complement(8146..8475)
                     /locus_tag="J1138_004151"
     CDS             complement(8146..8475)
                     /locus_tag="J1138_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial major subunit operon
                     transcriptional regulator"
                     /protein_id="HBA8455874.1"
                     /translation="MAQHEVITRGGDAFLLKLRESALSSGSMSEEQFFLLIGISSIHS
                     DRVILAMKDYLVSGHSRKDVCEKYQMNNGYFSTTLGRLTRLNVLVARLAPYYTDSVSA
                     IAEAASL"
BASE COUNT         2210 a         2285 c         1768 g         2251 t
ORIGIN      
        1 aatttattcc tgcctgattt ctgtataact gagatatccc ggcacagcgt acagataaat
       61 tccctcagat aaaagaacat aaatgctggc tgctgacaag tctgtgaaaa aggattatcc
      121 atactgctca gtaatcgcgg ataattcatc agctcccaca cgcctgtaga gccatccctg
      181 acagccctgg acattcatgt ctctggcaat acggaacaac gcttcatttt caacaccttc
      241 aagtattacg ccccggttat gacaggcaac agtgtctgcc acagcatgga gaagcggcat
      301 tgcactcctg tttttcaggg cataatgaaa aaactcatga cttattttta cccgttcaaa
      361 catcccggac agtccccaca ttaagcctgc atttcctgag ccgaaatcat ccagccagag
      421 ggtgaaacgt tctgacaaaa aacgtaacag ggggtcatca ccgggtttta acgccactgc
      481 cggaaagtcc tcactgacct caagcctgat atgtgttccc ggccgccccg ggatgccatg
      541 ttttctcaga tacagggcta ccacccggga aatattgaca ctcagcatta ttttcttctg
      601 cagacaccat ttatgaagcg tttccgcagt cttcaactga ctgatgagta ttttgtactg
      661 aatatccgga ggcgcacttc tgaaaaaaac agatgcgcag attttcctcc cgtcttttgc
      721 tgaacggata tccgagagca tttcaaaagc aaatattttc ccggtctgat tccatacagg
      781 ctcaaaaacc ggtgagagga tatattctgt tctgcctgat ttcacctcca gtatactgtt
      841 aactgcccct gacattactc ctccggaaat atgagcccct cccgaccaga aggtctggct
      901 ggataagcgc agttcttctg atttgatttt taaaccctca gatttaccat tatctttatg
      961 attactgaca acgctcagaa ccagatatac ggctcatact ggttctgttt atctttgaca
     1021 aagaccatat gacagactcg ttccgtcaca acaccggaaa caatactatt atcagctatt
     1081 tttctccaca aacagtgata cattaaatga aaaactggat ataaagagca gtaatatcat
     1141 taccgccaca actgcattct agttgtagct aaaagtcaca tttatcactg actggacagc
     1201 accgggcgtc ggacttgcct gaccggtcag agcataagag gccttcagcc ccagactcac
     1261 aggagagctg cctacctgtc ccagagacct cggttgtccg gccggaacca gccccgcgtt
     1321 gtccgacaac tgtacaccca caccgccggc ccctgatgtt gccgtatttg cgaatacagt
     1381 attaccactt cctgttacag aacctgacag ggtatagctg accgactgtg tctggtcgca
     1441 acgtaaagta agagggactg ttaccggccc tccgggatag gacggaaggt caactttgac
     1501 attgttcgat gagaccctgc atgtacctgt ctggatatag acatcattcg ttgcatagaa
     1561 acgccaggtg aaatttctct cccctgcctc ctgccccatg gtggaaaact tattaacata
     1621 aactgtggcc actaattctc cggcatgtat cagtttcccg aaaacgcccg gctcaggata
     1681 aaggtacatt tttgccggga aaggtttcca tacaccatat tgccagtaag cttctgttgg
     1741 ccttgtgtct tgctgcagcg gtaacgtctg actataccca gaaagcctgt ttgtaaagtc
     1801 cagccttccg tatgttttag catctaatga aggagaaaaa ccggttccct gtctgaccct
     1861 gagatagtca tagttctgac caaaggcatc ttcattctga cataacacca gttgatttaa
     1921 atccagaact gtaatggctt tatcatactg aactgttggg gaaagagtga tattgaagct
     1981 actgtctcca gactgaaaag tctgcccggt ctggttattt ctgcacaaca gtgcaaacga
     2041 tggctgggaa tatgccatta taaatgtgaa taaaacacag ccctcgagga cggtgctttt
     2101 cttaaagagg ggataatatt tacgcatagg tataggtgac agtaatttgt gaattaatac
     2161 ttcccgcacc agcattacct ttgcttacgg cacggacttt aagattgaat gtcgcctgcc
     2221 cattgctcac tgtaacaact tttgttgaac caactttcaa gggggtatta ccattacctt
     2281 gttcctgcag ctcaaccatg atattagtag catcccctgt atttctgtaa taatttgtat
     2341 tttctgcggt tccaccaaag gtggcagtaa cattgtttac attctgacaa ttcgtcaatg
     2401 tcaacgaaaa atttttccac tgagaggtgc tgttggctgc tatcaggtca gaagtgtaag
     2461 cgttatccaa aacaacggtg gcgtttgggt ttcctccatt accaatggta caggtacgtg
     2521 gcaataccct ccctgtaaca gtaatcgtcg tatccactgc ctgtgctgaa aacgcaatac
     2581 agttaataag accggtggcc aatataatag ctttcagttt catctgtttt cctctgtgtt
     2641 atatttactg atagtcaagt acaaaattaa ccaccgcatc aacattaccg gcctttaccg
     2701 gaagataagt ggctttcaga cgggcataaa acgctatggt ctgtgttccc ggcattaacg
     2761 caattgttgt cggcacatta attttcagct catgtttatc cttatcaaga agctgaatcc
     2821 ccacaccccg tgctccattt atgctgttaa tctgaataag ccccgcctcc tgattatctg
     2881 gtgtgcccgt aaatgtcagc ttcactgcac ggacagcact gccgcaggag gtcaacccga
     2941 catgaaatac cgacgcaggt gtggtatcac cagctgcggt aaactgtcgg ctgtcataac
     3001 ttcccagact gacatcaaag tttcgggagt taatgtcaac atcgcaggtg ttatcctgaa
     3061 cattgcctgt aatattcacc acagaaccca ctgcacagac atgacaggta acgaacatac
     3121 tgccggccag catgcaggag aatgtcactg ttttaataat atccttcacc atttccctgc
     3181 ctctttacct gcaactaacc gtgacatgac ttaatatctg tccggcgctt tccttcggta
     3241 atttataatc tgcagtacac tggtccgtgg tctgactccc ccatttcaca ctaacctgtc
     3301 ctgacagagg cattccgctc agataaacct gaccattctc tccggcaata ctgctccccc
     3361 cgtttctggc cgtaacaaca gagccaaaag gaactgcttt cccgtttctc atcagcgtca
     3421 tcagcacttt cacacccaca cgggttttat agtcagcccg cacaacggca ccatgcgtgg
     3481 gcacgacgct gaccacggta ttctctatat caacattatc tgcaagggtg ttggtatcca
     3541 gtgccacccg gttttcacga tagtctgttg cataaggcag tacagcatac cccctccagt
     3601 ctgtagacac ccccgtctgg ttttcaatgc gcgtatcaga tgcacctggt gcacggacaa
     3661 gaatcagcgt atctcccaga ggctggctca gtgtcagacc attggcatgt gccagaatac
     3721 cgccactcag cccataatac agttgccggt aacccccact gtgactgtat cctgcactgg
     3781 cattaccgta tccccctctg taattcagag aggcatatcc tgttttactg ctcccctcat
     3841 accctccccg ggtataacca ctctggatgc tgtatcccag attattgtcc cccagcaatg
     3901 tgccatacag ccctgctgta ctggcagcct gtccgtgagc ctccagggtc tgactgtaac
     3961 gggcactggc gtttctccat gcagatgtgc tgtccgaacg catccagtgg ctgaacggaa
     4021 tgctgacatc aaatgacagt atccggtcag tttcatgttg ccatgcattc cgggacaggc
     4081 tgtagttcag ggaccagctg atgtcattca cagatgagtt aaagccagca ttaagctgac
     4141 ggtccgtcct gtccgttccc cagtaactct ggtggctgcc actcagatac aacgttgacg
     4201 atttccccgt ctgctggctg atactcacct gcacccttcc ccgcttgttg taagccagat
     4261 tgtagtaatc cgtatacttc ggctgtatct gtatcactcc gtcctgggta agaacactgt
     4321 aaccactcat tttcttccag gcggtatcag caaagctgaa atagccccgt gtggagtagc
     4381 ggtatccgat taactggata tttgtccctg tttccgtcag tgacttgtta tacagaaaac
     4441 gatatgactg accgtcatgc cggctttcat ccggtaaccg ggcattcgca cgggtggcat
     4501 ccagtgaaac agcacccagc cagcccatgt ttttacccac ccccagattg aaggcacggt
     4561 agcgttcagc tatccaggct cctccataca ccgtccagcc tgccggcaga ccatacttca
     4621 gcgttccttc agcaaaacgg ggattatcct gctgatgtcc gccactgcga aactctccgg
     4681 cactgagagc atatttcaga tgtccttcac gttgcagtac aggcaccgaa gaccatggca
     4741 cattaaatcg ctgtacactg ccatcagctt cctgcagggt tacctgtaag tcacctccac
     4801 tcccggccgg ataaatatcg tttatctcga acgggcctgg aggtagcgta ctctggtaaa
     4861 tttcataccc gttctggcgg atactaatcc gggatgttcc ccgggaaata ccgtgaattg
     4921 tcggtgcata ccctctctgg ctgtccggaa ccatattgtc atcagaggcc agttgaatac
     4981 ccctgaaatt tacaccgtca aaaatatccc cccgggtata actgtccccc atcgtcagac
     5041 gggagcgcag gggaactata tcccgctcca gccaggtgtt aatgtgctgc cagttattct
     5101 ggctgtaccc ctttccggca ctgtaactcc agctggtgtt gtcccgaaga cgccagctcc
     5161 cgatattcag tcctgttttc agattcagat aggcataatc gctggtgcca ccatagctgt
     5221 cgcgcacccg attaccactg aagtcataat tcagcaatgc cgctgtaatt ccgttatccc
     5281 agtattccgg cgcaatataa ccacgggcct gattactcat atatatctga ggaacggtca
     5341 ggtacaatcg ctgctgcccg acatcaaacc ggaatgtggc accctgaatc atggaattca
     5401 gtgatgtgca ggtgtcagcc ggcagtctct ccatacccgg cacggcataa cggtttaccc
     5461 ccatcgcact catatgctcc ggactcaggc agggggcaag ttgcttcccg tcctgactca
     5521 tctgaaactg cacgtcacgg gttgagatat acgtatcatt cagataaata tccacccggt
     5581 acacgccggg cggaagctcc tgcccctgtg taaatgcaga caggtccgca accgcatccg
     5641 ggtcatcaga cagaaaacgc ggattaaaat agagctcagc ccgggccgga gaaacaatta
     5701 tcccgataaa aaatgccagc ggcagtatat taaatttcat tctgttatta cccccgccag
     5761 cagagccggt cagtgattta tcagacagga gttgtccgcc atctccggaa aacatgtcag
     5821 cccccacact gagcgaaaaa ataaaatcag cagccaaaca caaccagtac cagaaataac
     5881 tgagttactt caccaccccc ctcacctgtt cagtcagagc accataatcg ttaattgttt
     5941 tataagtaat atctcctccg gtgtatcctc cagggatatt cacagtgacc tttccctgtg
     6001 ggggtaccat ggtgttttca agacttttat ttccggcctt taagtcagtc actgtcagat
     6061 aatacggcgt cggattaagc agagtcagcc ctccgttttc acgagtgaac tctaatttac
     6121 ccggtgcctg ttctggcgga ataaccagtc cctgtggacg gtagagcagt ttaattctgc
     6181 tgacaattgc aaactgcaga taattctcgc cggttttcgc cttatccatg gccgggatgg
     6241 ccttgacatt cacccagaac aaactttccc tgtcttccgg catctgtccg tttgtggcat
     6301 caataattct cagggtattc tctttctttc cctgcatgga aaataacgga ggagtaatta
     6361 caaacctggc atcctttttt ccttcagcat tttcaatcca tgactgaata aggtaactac
     6421 ttttatcatc attatttgtt accgccagtt gtacctgttt ttgcccttca gggtaaatca
     6481 cacgggtggc acccagagca accccggcat agctctgcgg aatataaaaa aagactaaaa
     6541 atgatgccag aacggcccat atacgcatgt gcttcatcat gttattaaca gccttaatta
     6601 aatggcgtta tatctgccag ttacagatag gtcagagaaa accatacctg tccatttgcc
     6661 tttcccccgg tgacgggata atgtgtggcc ttatacctcg cctgcatatg cagtttcata
     6721 tctccgcggg taagatgcac ccagttctcc ggaggctgat taagttttac cagctctccg
     6781 ctttcattaa ataaagcgat agcgattccg tctgaagcat cattctcctc atccaccgac
     6841 agcaactccg gctcgtgaat atctgacacg ccataaaagg aaatccccac cccctgactg
     6901 acgacagtgc tgcagtcctg aaggtgaata tcaaagccca cagagtcccc atactcaccg
     6961 gcggcgtgaa atcgattgct gctgagttgc cccatatcca ctgtcatctg cctgtctgac
     7021 agggcaagac tgcatgcttc agcaattatt tttccctgga agcgcatgtt tcctcccgga
     7081 agcatgacat gccagtggtt tccggccagt gcagcgggag acagcaaaaa taacattact
     7141 gccattttcg atattttcat taatctcacc cccgcatgga taaaaacagc cctccctggc
     7201 cgggtatata tccgttacac tgttctgata ttactggtac tgaactttaa aggtggcgtc
     7261 ggcgttggca ataccagcac cagctgcacc acctacagca acatagcgag cctggaatgg
     7321 gattttaatt gtgccatcag taatggtata ttctgagcta actgtaaccc catcgaactt
     7381 gactgctgca cctttataat ccattacttt tatcccgaca tttgttgctg aacccgctga
     7441 gctaccctgc aaggataaaa tgtctgcctg cccaccctgg gggccagaaa aaataattga
     7501 agctcctgat gaggcggctg agctacaatc atttaattca atatcaaaac caacaggtga
     7561 actcattttt ccgtcagcat ctaattttgc cgctcgaact tgccccaatt gaactgtctg
     7621 gtctgctgag ttcatattca cagcgcatgc agcctcaaca acctctcctt taaagtgaac
     7681 agtaccgcca ttgacgattg cggcagacgc acttgtcgca acacccaggg tcagagctga
     7741 aaatacagcc atggagatga attttaactt catatttaat tcctgttttt aattgaacta
     7801 tgcacaccat atctgtcatc acaacagaca caatcaatat cccgagcgac gggaatcttt
     7861 ttcaggagaa cttcataaat gcaatgtgag aaatacactc aggcagcatt tatgacattg
     7921 tctcccgata atattttccg gataaccgac aggaatcttc tctatagata agattccctg
     7981 gatgataagc tatgggttag tgttcctgtc tccacaccga atgctttagc aaaagcagag
     8041 aagctgaaag catacctcgt tgataacacc cgtactctca tttgactaca ggcttatatt
     8101 tctcgcgtat tatgtattgc cccggcgttt tttattgtcc gtattttaca gggaagcagc
     8161 ttcagctata gctgatacac tatcagtata ataaggagca agtctggcaa cgaggacatt
     8221 caacctggta aggcgcccaa gcgttgtact gaaatatcca ttattcatct ggtatttctc
     8281 gcagacatct ttacgggaat gaccactgac cagataatcc ttcatagcga gaataactct
     8341 gtcactgtga atagaggaaa tacctatcag taaaaaaaat tgttcttcag acatggagcc
     8401 ggatgacagc gcgctctcac gtaattttag taaaaaagca tcccctcccc gggtaataac
     8461 ttcatgctgt gccattcgat actccccacc ccgccaccta cagggctgcc ttaa
//
LOCUS       DADTFG010000182         8255 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.183, whole genome shotgun sequence.
ACCESSION   DADTFG010000182 DADTFG010000000
VERSION     DADTFG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.183"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            300..1283
                     /locus_tag="J1138_004152"
     CDS             300..1283
                     /locus_tag="J1138_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HBA8455875.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAMTNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVML
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1355..2503
                     /locus_tag="J1138_004153"
     CDS             1355..2503
                     /locus_tag="J1138_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HBA8455876.1"
                     /translation="MLIKVKSAVSWMCARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2527..4203
                     /locus_tag="J1138_004154"
     CDS             2527..4203
                     /locus_tag="J1138_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter KpsD"
                     /protein_id="HBA8455877.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQ
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4213..4953
                     /gene="kdsB"
                     /locus_tag="J1138_004155"
     CDS             4213..4953
                     /gene="kdsB"
                     /locus_tag="J1138_004155"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBA8455878.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMQEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4950..6977
                     /locus_tag="J1138_004156"
     CDS             4950..6977
                     /locus_tag="J1138_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HBA8455879.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERADIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSQVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSNLFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQKMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKVILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTIADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            7012..8181
                     /locus_tag="J1138_004157"
                     /pseudo
     CDS             7012..8181
                     /locus_tag="J1138_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
BASE COUNT         2146 a         1959 c         2135 g         2015 t
ORIGIN      
        1 tgagaaacat tttaataaat gataaaaatc ctaaattcct tgttcataat gtaggggtgt
       61 gttaaatagg tgggcaaaat ttggttcccc ttctcgcctg taatattgca gcatcttcgc
      121 acctccatga gacattgcga cttaataaga aggtgataag tcctgcatat aagcatggac
      181 tgaccatggt tttatattca ttaaaaattt tgttacaacc cattgattta gcataaataa
      241 attatagtgg gttcgggttt gttgtgactg tggcattatt tccgtgcaaa ggagctgata
      301 tgtctgaaag acatttacct gatgaccaga gcagtactat cgatccatat ctaattacct
      361 ctgttcgcca gactctggca gaacaaggcg cagcattaca aaacttgtct aaacaactgg
      421 attccgggca gtaccagcgt gtccttaatt tgataatgaa ctgtaaaggg cacgttattc
      481 tttcgggaat gggtaaatca gggcatgtcg gtcgtaaaat gtcagcgacg ctggcctcta
      541 cgggtacgcc tagtttcttt attcatcctg cagaagcttt ccatggcgat ctgggcatga
      601 ttacgcctta cgatcttctg atccttattt ctgccagcgg tgaaacggat gaaatcctca
      661 agctagttcc ttcactgaaa aatttcggca accgaattat cgccatgacc aataatggaa
      721 attccacgct ggcgaaaaat gctgatgccg tgctggaact ccacatggcg aatgaaacct
      781 gcccgaataa tcttgcacca acaacgtcta ccacgctgac gatggcgatc ggcgatgcgc
      841 tggcgattgc catgatccac caacgcaaat ttatgccgaa tgattttgcg cgctatcacc
      901 cgggcggttc attaggtcgt cgcctgctga cccgcgttgc tgatgtcatg ctgcatgatg
      961 ttcctgcggt acagctggat gcgtcattta aaaccgtgat tcaacgtatc accagcggat
     1021 gccagggaat ggtgatggta gaagacgcag aaggtgggct agcgggcatt atcaccgacg
     1081 gtgacctgcg tcgctttatg gaaaaagagg attctctgac atccgccacg gctgcgcaga
     1141 tgatgacacg tgaaccgctg acgctaccgg aagacaccat gatcattgaa gcggaagaaa
     1201 aaatgcaaaa acaccgcgtc tcaacgttat tggtgaccaa caaggcaaat aaagtcactg
     1261 gccttgtgcg cattttcgac taattaagca acggggtgcg agaggttagt ctctcattga
     1321 gtttgcgccc cactgaaact ttgataatcg ttacatgttg ataaaagtga agtctgccgt
     1381 atcctggatg tgtgctcgtc tatctgccat ctcactggca gatatccaaa aacacctggc
     1441 gaaaatcatc attctggcac cgatggcggt gctgctgatc tatctggcta tcttcagcca
     1501 gcctcgctat atgagcgagt cgaaagtcgc cattaaacgc tcggatgatt taaacagcgg
     1561 cagcctgaat tttggtctgc ttctgggtgc ctctaacccc agttccgcag aagatgcgtt
     1621 gtatctgaaa gagtacatca actcgccgga tatgctggcg gcgctggata agcaactaaa
     1681 ttttcgtgaa gcgtttagcc acagcgggct cgattttctt aatcatctta gcaaggatga
     1741 aaccgcagaa ggcttcctga agtactacaa ggaccgtatc aacgtctcgt atgacgataa
     1801 aaccggatta ctgaatattc agacgcaggg ctttagcccg gagtttgcgc ttaagtttaa
     1861 ccagaccgtg ctgaaagagt cagagcgctt tatcaatgag atgtcacatc gcatcgcgcg
     1921 tgaccagctt gcctttgcag aaacggagat ggaaaaggca cgccagcgtc tggacgccag
     1981 caaagcggaa ttgctctctt atcaggacaa caacaacgtt ctggatccac aggcacaggc
     2041 acaggcggcg agcacgttag tgaatacgct gatgggccag aagatccaga tggaagcgga
     2101 cctgcggaac ttgctgacgt atctgcgtga ggacgccccg caagttgtga gtgcgcgtaa
     2161 tgcgattcag tcattgcagg cacaaattga cgaagaaaaa agcaaaatca cagcgccgca
     2221 gggtgacaag ctaaaccgta tggcggtgga ttttgaagaa atcaaatcaa aagtagagtt
     2281 caacaccgag ctgtacaaac tgaccctgac ctccattgaa aagacccgtg tagaagcggc
     2341 tcgtaagctc aaggtgctgt cagtgatcag ttcgccacag ttgccgcagg aatcgtcttt
     2401 tccaaatatc ccttatttga tcgcctgctg gttactggtg tgctgcctgc tgttcggcac
     2461 cctgaaactg ttgctggctg ttattgaaga tcaccgagac taacgctgtc gctgaatgag
     2521 tttgtgatga aattatttaa atcaatttta ctgattgccg cctgtcacgc ggcgcaggcc
     2581 agcgcggcca ttgatattaa cgctgaccca aacctgacag gagccgcgcc gcttaccggt
     2641 attctgaacg ggcaacagtc ggatacgcaa aacatgagcg gcttcgacaa taccccgccg
     2701 cccgcaccgc cggtggtaat gagccgtatg tttggtgctc aacttttcaa cggcaccagc
     2761 gcggatagcg gtgcgacggt aggattcaac cctgactata ttctgaatcc gggtgatagc
     2821 attcaggttc gcttgtgggg tgcgttcacc tttgatggtg cgttacaggt tgatcccaaa
     2881 ggtaatattt tcctgccgaa cgttggtccg gtgaaagttg ctggcgtcag taatagtcag
     2941 ctaaatgccc tggtcacatc caaagtgaag gaagtatacc agtccaacgt caacgtctac
     3001 gcctccttat tacaggcgca gccagtaaaa gtgtacgtga ccggatttgt gcgtaatcct
     3061 ggtctgtatg gcggcgtgac gtctgattcg ttactcaatt atctgatcaa ggctggcggc
     3121 gttgatccag agcgcggaag ttacgttgat attgtggtca agcgcggtaa ccgcgtgcgc
     3181 tccaacgtca acctgtacga cttcctgctg aacggcaaac tggggctttc gcagttcgcc
     3241 gatggtgaca ccatcatcgt cgggccgcgt cagcatactt tcagcgttca gggcgatgtc
     3301 tttaacagct acgactttga gttccgcgaa agcagcattc ccgtaacgga agcgttgagc
     3361 tgggcgcgcc ctaagcctgg cgcgactcac attacgatta tgcgtaaaca ggggctgcaa
     3421 aaacgcagcg aatactatcc gatcagttct gcgccaggcc gtatgttgca aaatggcgat
     3481 accttaatcg tgagcactga ccgctatgcc ggcaccattc aggtgcgggt tgaaggcgca
     3541 cactccggtg aacatgccat ggtactgcct tatggttcca ctatgcgtgc ggttctggaa
     3601 aaagtccgcc cgaacagcat gtcgcagatg aacgcagttc agctttatcg cccatcagtg
     3661 gctcagcgtc agaaagagat gctgaatctc tcgctgcaaa agctggagga agcatcactt
     3721 tctgcccagt cctccaccaa agaagaagcc agcctgcgaa tgcaggaagc acaactgatc
     3781 agccgctttg tggcgaaagc acgcaccgtt gttccgaaag gtgaagtgat cctcaacgaa
     3841 tccaatattg attctgttct gcttgaagat ggcgacgtca tcaatattcc ggagaaaaca
     3901 tcgctggtta tggttcatgg cgaagtgctg ttcccgaacg cggtgagctg gcagaagggt
     3961 atgaccaccg aggattacat cgagaaatgt ggtggcctga cgcagaaatc gggtaacgcc
     4021 agaattatcg tcattcgtca gaacggtgca gccgtcaacg cagaagatgt ggattcactc
     4081 aaaccgggcg atgagattat ggttctgccg aaatatgaat cgaaaaacat tgaagttacc
     4141 cgtggtattt ccaccatcct ctatcagctg gcggtgggtg caaaagtgat tctgtctttg
     4201 taaggagttg aaatgagcaa agcagttatt gtcattccgg ctcgttatgg ctcatcgcgc
     4261 ctgccgggta agccactgct cgatattgtt ggtaaaccga tgatccagca tgtttacgag
     4321 cgtgcgttac aggtggcggg cgttgcggaa gtttgggtgg caacagacga tcagcgtgtt
     4381 gaaaaggccg tgcaggcatt tggcgggaaa gccatcatga cgcgcaacga tcatgaatcc
     4441 ggcaccgatc ggctggtcga ggtgatgcat aaagtcgagg cagatatcta cattaacctg
     4501 cagggcgacg aaccaatgat tcgcccgcgg gatgtagaaa cgctgttaca aggaatgcgt
     4561 gatgaccccg cgttgccagt ggcaacgcta tgccacgcga tttctgccga agaagcgacc
     4621 gagccaagca cggtaaaagt ggtggtaaat acccaccagg atgcgcttta tttcagccgt
     4681 tcgcctattc cgtatccgcg taatgctgaa aaagcgcgct acctgaaaca cgttggtatt
     4741 tacgcttatc gtcgcgatgt gctgcaaaac tacagccagt taccggagtc catgcaggag
     4801 caggcagaat cactggagca gctgcggttg atgagcgcgg ggatcaacat ccgtacattt
     4861 gaggttgccg caaccggtcc gggcgtcgac accccggcat gcctggaaaa agtgcgcgcc
     4921 ctgatggcac aggaactggc tgaaaacgca tgattggcat ttactcgcct ggcatctggc
     4981 gtattccgca tctggagaaa tttctggcgc aaccgtgcca gaaactttct ctgctgcgcc
     5041 ctgttccgca agaagttgat gctatcgccg tgtggggaca tcgtcccagc gcggcgaaac
     5101 cagtcgccat cgccaaagca gcgggaaaac ccgtcattcg tctggaagat ggatttgtgc
     5161 gttcgctgga tcttggcgtc aatggcgagc cgccgctttc tctggtggtg gatgattgtg
     5221 gcatttacta cgatgccagc aagccttcag cactggagaa actggtacag gataaagccg
     5281 gaaatacagc tctgataagc caggccagag aagcgatgca caccatcgtg accggggatt
     5341 tgtcgaaata taacctggca cctgcgtttg tggctgatga gtcagaacgt gcagacatcg
     5401 ttctggttgt cgatcagaca tttaatgata tgtcagtgac gtatggcaat gctggcccgc
     5461 atgagtttgc tgccatgctg gaagccgcga tggcggaaaa tcctcaagcc gaaatttggg
     5521 tgaaggtgca cccagatgta ctggaaggaa agaaaacagg ttatttcgct gatctgcgtg
     5581 ccacgcaacg agtacgtttg attgccgaga atgtcagccc gcagtcgcta ttgcgacacg
     5641 tttcccaggt ttacgtcgtg acctcccagt acggctttga agccttgctg gcaggaaaac
     5701 cagtaacatg tttcggccag ccttggtatg caggttgggg tttaaccgac gatcgtcatc
     5761 cacagtccgc tttgttatct gcccgacgcg gttctgccac gctggaggaa ctttttgccg
     5821 ctgcatacct gcgttactgt cgctatatcg atccgcaaac gggagaagta agcaatctat
     5881 ttaccgtgct gcaatggctg caattacaac gtcggcatct gcaacagcgt aatggttatt
     5941 tatgggcgcc aggcttaacg ctgtggaagt cagcgatcct gaaacctttc ttgcaaacgg
     6001 caacaaaccg gctgagtttt tctcgtcgct gtactgcggc gagcgcctgc gtggtatggg
     6061 gtgtaaaggg agaacagcaa tggcgagccg aagcgcagcg aaaatcactg ccgttatggc
     6121 gaatggaaga tggttttctg cgttcatccg gacttggctc tgacctgctg ccgccgctat
     6181 cgctggtgct ggataaacgc ggtatttact atgacgccac gcgccccagc gatctggaag
     6241 tgctgctgaa tcatagccag ctaacgctgg cgcagaagat gcgagctgaa aaattacgcc
     6301 agcgactggt tgaaagcaaa ctgagtaagt acaacctggg agccgatttc tctctaccag
     6361 ccgaagccaa agataaaaaa gttatcctgg tgccgggtca ggtagaagac gatgcctcaa
     6421 ttaaaaccgg caccgtttct attaagagca accttgagtt attacgcaca gtacgcgagc
     6481 gtaatccgca cgcttacatt atttataaac cgcacccgga tgtactggtg gggaatcgca
     6541 agggcaatat tccgacagaa ctaattgctg aactcgctga ttatcaggca ctggacgcag
     6601 atattattca atgcattcag cgcgcagatg aagtgcacac catgacatca ttgtccgggt
     6661 ttgaagcgtt attacatggc aagcaagttc attgttacgg cctgcccttc tatgccggtt
     6721 ggggtttaac cgttgatgaa catcattgcc cgcgccgcga gcaaaaatta acgatagcag
     6781 acttgatcta tcaggcgttg attgtttacc caacatatat tcacccaaca cggctacagc
     6841 ctattacggt tgaggaagct gcggaatatt taatccagac gccgcgtaag ccgatgttta
     6901 ttacccgaaa aaaagcggga cgggtaatac gctattaccg caaattaatt atgttctgca
     6961 aggtcagatt tggctaaaca atttcatagt tgactattac gcaaattatt tatgcaaggt
     7021 aatgcactaa ccgttttatt atccggtaaa aaatatctgc tattgcaggg gccgatggga
     7081 ccttttttca atgacgtcgc cgaatggtta gagtcattag gacgtaacgc tgtgaatgtt
     7141 gtattcaacg gtggggatcg tttttactgc cgccatcgac aatacctggc ctactaccaa
     7201 acgccgaaag agttccccgg atggttacgg gatctccacc ggcaatatga ctttgatacc
     7261 atcctctgct ttggtgactg ccgcccattg cacaaagaag caaaacgttg ggcaaagtcg
     7321 aaagggatcc gctttctggc atttgaggaa ggatatttac gcccgcaatt tattaccgtt
     7381 gaagaaggcg gagtaaacgc atattcatcg ctaccgcgcg atccggattt ttatcgtaag
     7441 ttaccagata tgcctgcgcc gcacgttgag aacttaaaac cttcaacgat gaaacgtata
     7501 ggtcatgcga tgtggtatta cctgatgggc tggcattacc gccatgagtt ccctcgctac
     7561 cgccaccata aatcgttttc cccctggtat gaagcacgtt gctgggttcg tgcatactgg
     7621 cgcaagcaac tttacaaggt aacacagcgt aaggtattgc cgaggttaat gaatgagctg
     7681 gatcagcgtt attatcttgc cgttttgcag gtgtataacg atagccagat tcgtaaccac
     7741 agcaattata acgatgtgcg tgactatatt aatgaagtca tgtactcatt ttcacgtaaa
     7801 gcaccgaaag aaagttattt ggtgatcaag caccatccaa tggatcgtgg tcacagactc
     7861 tatcgaccat taattaagcg gttgagtaag gaatatggct taggggaacg agtcatttat
     7921 gtgcacgatc tcccgatgcc ggaattatta cgccacgcaa aagcggtggt gacaattaac
     7981 agtacggcgg ggatctctgc gctgattcat aacaaaccac tcaaagtgat gggcaatgcc
     8041 ctgtacgaca tcaaaggctt gacgtatcaa ggacatttgc accagttctg gcaggccgat
     8101 tttaaaccgg atatgaaact gtttaagaag tttcgggggt atttattggt gaagacacag
     8161 gttaattggg tttattatgg agatgtaaag aaagggttaa gatggattat aaggacagat
     8221 ttttcttaca tcaaaaataa taagatacaa tcacc
//
LOCUS       DADTFG010000183         8067 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.184, whole genome shotgun sequence.
ACCESSION   DADTFG010000183 DADTFG010000000
VERSION     DADTFG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.184"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            89..478
                     /locus_tag="J1138_004158"
     CDS             89..478
                     /locus_tag="J1138_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HBA8455880.1"
                     /translation="MDVNPSLTMENISELSTSSEPSQQGVFPVICTRLHPGSVMKRQL
                     LTGWGPVFIIGDDPFSLRWMSEHLEILKSLNALGLVVNVESVERMEVLQQRADGLLLL
                     PVICDNFVQALQLNAYPVLITEMEISQ"
     gene            504..698
                     /locus_tag="J1138_004159"
                     /pseudo
     CDS             504..698
                     /locus_tag="J1138_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(749..1519)
                     /gene="yafV"
                     /locus_tag="J1138_004160"
     CDS             complement(749..1519)
                     /gene="yafV"
                     /locus_tag="J1138_004160"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBA8455881.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
     gene            1673..2146
                     /gene="ivy"
                     /locus_tag="J1138_004161"
     CDS             1673..2146
                     /gene="ivy"
                     /locus_tag="J1138_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HBA8455882.1"
                     /translation="MGRISPGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2189..4633)
                     /locus_tag="J1138_004162"
     CDS             complement(2189..4633)
                     /locus_tag="J1138_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBA8455883.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLSFTRLDELAHNALAKVLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4873..5451
                     /gene="lpcA"
                     /locus_tag="J1138_004163"
     CDS             4873..5451
                     /gene="lpcA"
                     /locus_tag="J1138_004163"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBA8455884.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5656..6423
                     /locus_tag="J1138_004164"
     CDS             5656..6423
                     /locus_tag="J1138_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBA8455885.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLVDELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6394..7134)
                     /locus_tag="J1138_004165"
     CDS             complement(6394..7134)
                     /locus_tag="J1138_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBA8455886.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7290..7472)
                     /locus_tag="J1138_004166"
     CDS             complement(7290..7472)
                     /locus_tag="J1138_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HBA8455887.1"
                     /translation="MTRLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTD
                     KLLRFERTGTHAALFG"
     gene            complement(7696..7956)
                     /gene="dinJ"
                     /locus_tag="J1138_004167"
     CDS             complement(7696..7956)
                     /gene="dinJ"
                     /locus_tag="J1138_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HBA8455888.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
BASE COUNT         1990 a         2096 c         1986 g         1995 t
ORIGIN      
        1 gagtttatac gtaataatat tgttattttc ccttcagttt agtgcagcca ttactggcaa
       61 ggaaagtgag gttgtttcac cattattaat ggatgttaat ccttcactaa caatggaaaa
      121 tatttctgaa ttatccacat catcggaacc atcgcaacag ggggtatttc ctgtaatatg
      181 tactcgactt catcctggct ccgtaatgaa acgtcagtta ttaaccggct gggggcctgt
      241 ttttataatt ggtgatgatc ctttttctct gcgctggatg agtgaacatc tggagatcct
      301 taaatcgcta aatgcccttg gtttggttgt taatgttgaa tcagtagaac gaatggaggt
      361 attacagcag cgggctgatg ggttattgtt attgccagtt atctgcgata attttgtgca
      421 agctctgcaa ttaaatgctt atccggtatt aatcacagag atggaaatat cacaatgatt
      481 aataacataa ttactgctaa taatatggtg attttgaaaa tggtaatcct gttcacgaca
      541 ccataatcag agttatgtcc tgtatccgtt cagtaaaatt tcacgaatgc tttattaacc
      601 ggatgcgtga atgtcattct tcagacgaca tagacgtcat tgcgattgat ggaaaagcac
      661 ttccacactc ctgtgataaa agtcgtcgca ggagagcggg ctttcgtagt cttccccgac
      721 tctccccggc cctaaacaca acccccactc accacaacct aaactcatcc gcatcccgcc
      781 atgccggaaa cttttctcta tattcccgca atgccatcat cgacagctcc gcatcaatgc
      841 gcgttgcctg atgcgcgtcg gcagtagcga taatctcgcc ttgcggatta atcacccggc
      901 tgtcaccgcg ataatggcag ccgttgccat cgctgccgac gcgattgcat cccgccacat
      961 acgcttgatt ctcaatcgcg cgggctgtca gcaatgcctg ccagtgcaga gagcgcggag
     1021 caggccagtt ggcgacgtac agggcgaggt cataatcgtt gagattgcgc gaccacaccg
     1081 gaaaacgtaa gtcgtagcac accagcggca aaatacgcca gccgcgccat tccacaatca
     1141 ctcgcgcatt gcccgcttta taatgtagat gctcatctgc catgcggaac agatgacgct
     1201 tatcataaaa atgtaccgtg ccgcccggct caaccagcaa aaagcggtta accgaaccag
     1261 actccgtttg taatgcaaca ctgcctgcaa tcagcgcatt gcactgctgc gctttagcgg
     1321 tcatccagtt cactacgtca tcttgtgcta gcgacgaagc tgccgcttcc atggcaaagc
     1381 cgctggtaaa catctccggt agaacgatca catcgcgccc ggtaatacct tccagttgac
     1441 gatcaaaatg gcgcaggttg gcaggaccat ccatccacac cagtggttgc tgcaaaagcg
     1501 taatcttcaa accaggcacg gtgtacaact cctttatgcg aagggtttta taactctaac
     1561 accttatcag gcagttgcct tagcgcagaa taaattgata acaaatgctg atattggaaa
     1621 tatctgattt gcaaattatc gtgttatcgc caggctttag ggagttaatc acatgggcag
     1681 gataagcccg ggaggaatga tgtttaaggc aataacgaca gtcgccgctc tggtcatcgc
     1741 caccagtgca atggcgcagg atgatttaac cattagcagt cttgcaaagg gcgaaaccac
     1801 caaagcggcg tttaatcaga tggtgcaagg gcataagctg cctgcctggg tgatgaaagg
     1861 cggtacttat actcccgcac aaaccgtgac attgggagat gagacgtatc aggtgatgag
     1921 cgcgtgcaaa ccgcatgact gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc
     1981 taatcagatg acggggctgt tctcgaccat tgatgagaaa acgtcgcaag agaaactcac
     2041 ctggttgaat gtgaacgatg cgctttctat tgatggtaaa acggtgctgt tcgcggcgtt
     2101 gaccggcagc ctggaaaacc atccggatgg ctttaatttt aaataattag ccgataaaaa
     2161 aacggagccg aaaggctccg ttattcaatt acgcggcttc aactttccgc actttctccg
     2221 gcaactttac cggcttcgtc gccagctctt ccggatcaaa gtcatcaacg ttaatactgc
     2281 gcagacggct ttcttcagct ttcaccagaa tagcggcttc atctttatca atcagcacct
     2341 tcgccagcgc gttgtgcgcc agttcatcca gacgggtaaa cgacaggttt ttacccagct
     2401 ctttacagat ccgctgatga atcgggtcgg cggcaatcac atccactagc gcctcttcca
     2461 gtaaaccaac cgggttatgc tcgctcggcg tcaggtactg accgcgacca atgcgggaac
     2521 gggtggcgtt cggcacttgt aaaatcttcg ccaccttatg atccagcttg tcagaaggtg
     2581 ccagataatg acgtccggtc gggaagatca ccacattcag cagcccggca accacgcggt
     2641 tcgggaagtt ttgcagtaaa tcgtccatcg cctgttctgc ctgatacagc gcatcctgta
     2701 cgccccagtg caccagcggc agatcggctt cattacggcc ttcgtcgtca taacgcttca
     2761 gaacggcaga ggcgaggtaa agctggctta aaatatcccc cagacgtgcc gagatacgct
     2821 cgcgacgttt caggctgccg cccagcacag ccatcgagac atcagaaagc agggcgaggt
     2881 tggcgctcag gcggttcagg tgctgatagt agcgtttagt ggcatcgccg gttggcgtgc
     2941 tgctggttaa accgcgcgtc aggcccagcc agaagctgcg aactttgttg ctaccgacgt
     3001 gaccgatatg tttaaacagg agtttatcga acgcgttgac gtcattgttc ttcgccgctt
     3061 ccatctcttc cagcacgtac ggatggcaac gaatcgcccc ctgaccgaaa atcatcatgc
     3121 tacgggtcag aatatttgcc ccttcaacgg tgatggcaat cggtgccccc tggtaagcac
     3181 gggccaggaa gttgctttgc ccgagcataa tgcctttgcc accagtgata tccatcgcat
     3241 caataatcga ctgctgcccg cggtgggtac agtgatactt aacgatagcc gacagcacgg
     3301 cgggtttttc accgagcata atgccgtagg taatcagcga tgccgcagca tccattacat
     3361 aggcattacc ggcaatacgc gccagcggct cttcaatccc ttccatctta ccaatagaaa
     3421 ttttgaactg acggcgaatg tgcgcatacg cacccgttgc cagtgctacc gatttcacgc
     3481 cgccggttga gttggaaggc agggtgatgc cgcggcctac cgagaggcac tccaccagca
     3541 tccgccagcc ttgcccggcc attttcggcc cgccgatgat gtaatcgatc ggcacgaaca
     3601 catctttacc gcgcgtcggt ccgttctgga acggtacgtt cagcgggaag tggcgacgac
     3661 caatttccac gcccggcgtg gtggtcggga tcagcgcaca ggtaatacct aaatcttctg
     3721 caccgccgag taatttctcc gggtcggaga gtttaaacgc cagaccaagc acggtcgcaa
     3781 tcggtgccag cgtaatgtag cgtttgttcc aggtcagacg cattcccagc acctgctggc
     3841 cctgccattc gcccatgcag acaatcccgg tatccggaat cgcgcccgca tcggaacccg
     3901 cttccgggct ggtcagtgca aagcaaggga tctcctgacc acgcgccaga cgcggcagat
     3961 agtgattttt ctgctcgtca gtgccgtaat gttgcagcag ctcgcccggg cctaatgagt
     4021 tcggcacgcc gacggtaatc gccaggatcc cgctcacgcc ggagagtttt tgcagcacgc
     4081 gagactgggc ataagccgag aactccagcc cgccgtactc ttttttgatg atcatcgcga
     4141 agaaacgatg ctctttaagg tacgcccaca actccggcgg caggtccgcc agctcatgag
     4201 taatctggaa atcattcgcc atccggcagg cttcttctac cggaccgtcg agaaacgctt
     4261 gttcttcagc ggtcaggcgc ggctgcggat agttatgcag ctttttccag tccggcttgc
     4321 cctggaacaa gtcgccctcc caccaggtgg tgcccgcatc aatcgcttct ttctcagtgc
     4381 gcgacatcgg cggcatcacc ttacggaaac cgcgaaatac cggtgcggaa atcatcgact
     4441 tacgcatagg cgcaaagtta aatggcacga ggataatggc cagaggcacc agtacccacg
     4501 ccgaccacag accagcaacg ccgaggacgg ctgtccaggc gagcaaaatc agactgctga
     4561 taaataagct cacgcggtga tagaacaatg cgccgagcag gacaaccgta gcgagaatac
     4621 tcaaaatcat cataacgaaa agccccttac ttgtaggagg tctgaccact tgtgatgata
     4681 tggttgtagt ggatgtgaaa acatttagca atgcgtttac aatataatta caacaaagct
     4741 cacattgttg ctgtttttat ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc
     4801 cgtacttctc gcttttggcg gtatccggta cactgcattt tgtctattac atttatgctg
     4861 aaggatatcc tcatgtacca ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg
     4921 ctggctaact ttttaaaaga tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg
     4981 ttagcagaca gttttaaagc cggtggcaag gtgctttcct gtggcaacgg cggctcccat
     5041 tgcgacgcta tgcactttgc cgaagagttg accggtcgct accgtgaaaa ccgtccgggc
     5101 tacccggcga ttgccatttc tgacgttagt catatttcct gcgtcggtaa cgattttggt
     5161 ttcaatgata ttttctcccg ttacgttgaa gcggtaggtc gcgaaggcga tgtactgctg
     5221 gggatctcta cctccggtaa ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag
     5281 aaggggatga aagtgatcac cctgaccggt aaagatggcg gcaaaatggc tggcacggcg
     5341 gatattgaaa ttcgcgttcc gcactttggt tatgccgacc gcattcagga gatccacatt
     5401 aaagtgatcc atatcctgat ccagttaatt gaaaaagaga tggttaagta agtctggcgt
     5461 aggccggata aggcgtttac gccgcatccg gcatttgtgc tctgatgcct gatgcgacgc
     5521 tgacgcgtct tatcatgcct acaatctgca cccgaaccgt aggccgaata atgcgttcac
     5581 gccacatccg acctgaaaat tcttaaatca atcttcgccg ggggccatgc gctcccgctg
     5641 ttgtggaggt tacccatgtg cgaattgctc gggatgagtg ccaacgtccc taccgatatc
     5701 tgctttagtt tcaccggact tgtacagcgt ggtggtggaa ccgggccaca taaagatggc
     5761 tggggcatta ccttttacga aggtaaaggc tgtcgcacat ttaaagatcc gcaacccagc
     5821 tttaattccc ccatcgccaa acttgtccag gactacccga taaaatcctg ttcggtggtg
     5881 gctcatattc gccaggctaa tcggggcgag gtggcgctgg aaaatactca cccgtttacc
     5941 cgcgagttat gggggcgtaa ctggacttat gcccataatg gacaactgac gggctacaaa
     6001 tcactggaaa ccggcaactt ccgcccggtc ggtgaaaccg atagcgaaaa agccttttgc
     6061 tggctcctgc ataaattaac gcagcgttac ccgcgcacac cgggcaacat ggcggcagtg
     6121 tttaaatata tcgcctcact ggtggatgaa ctgcggcaga agggcgtttt caacatgcta
     6181 ctttcggatg ggcgctatgt aatggcgtat tgctcgacta atttacactg gatcacccgc
     6241 cgcgcgccgt ttggcgtggc aacgttgctg gatcaggatg tggaaatcga cttcagctcg
     6301 cagaccacac cgaatgatgt ggtcacggtg attgcgacac agccgctgac gggcaatgaa
     6361 acctggcaaa agattatgcc aggtgaatgg cgcttatttt gcctcgggga gcgtgtagtt
     6421 tgatgccagt tgtggctgca caacttcgtg gcttaacggc ttgctgacca cgtaacggcc
     6481 attgaccaca gaaacggtcg gtggcttacg ggtttgctca aagtagtcgt agcctggctt
     6541 cagttgctcc cagaagtcct taaagttgga atatttatgg cgcttcatat tggcgtcggt
     6601 catacggaac gggtaaatac tcacttgcac gctcggctga ccaaacacca gcgcaccagt
     6661 aacgaactgg aagatctcat caataccctg attggtcatc gcgtagcagc cgatggaaac
     6721 acaatcgccg tgaatcatca ggtatttccc ttcataacca tgcgcacggt cataggcgtt
     6781 tgggaatcca atattaatcg ctttgtagta acggctgtct ggttttaact gattacgctg
     6841 gacgctataa aacccttccg ggcttttgaa atcgccctga cgctgttttg gccctaagcc
     6901 gccggaatat ttacagattt tatagctgtc gagcagttga tattgctcgc ccattttgac
     6961 gtagagatcg agcgtacgtt cttccttgaa gatctggatg tagacagggg atcccatcaa
     7021 ctgctgctta tactctttgc tcactggcgt ggtcgagcta ctgctgccca gcaaaccggc
     7081 aaacgaaacg cacgggatca acaacatcgc aagaattaat gcgattttac gcatactgct
     7141 tattccttga taaaacggtt acacacgcca ggacggcaaa atggatccca aatcggaata
     7201 gtctggattg ggaaggctca cattatcacc aaaagagttt tacgcaagcc tgtcggcgca
     7261 gggtttacaa atttcatcgg cagcgggctt tacccaaaga gcgccgcgtg agttccagtt
     7321 ctctcaaatc gtaaaagttt atcggtaagt ttgtaaatca ggatccagtc cggctcgaca
     7381 tgagcatcgc gataaccttt ccatgaacct tgcagcgggt ggtctttata aacagctgga
     7441 agcggtaaag tattattgat aagacgcgtc ataagatatt tcaatttata ctcgtcatac
     7501 ttcaagttgc atgtgctgcg actgcgttcg ttcaccccag tcacttactt tagtaagctc
     7561 ctggggattc actcgcttgt cgccttcctg caactcgaat tatttagagt atattcatat
     7621 ccttatgacg cttttgtgca agttttacat cctttgaaaa ttgtcccgag tattcaatat
     7681 ccctttgaat catatttaaa ttcctaattt atcaaataaa tcatcggcgt ctttggcctt
     7741 atgaacatca acgccagctt cgctgttttt aattgattga atggttaatt gattaggctc
     7801 gcgtaaatca aacggcaatg ccttttcacg cgcgactttt gtgagggtta tgcgaaccag
     7861 gtcagagatg gtcagcccca tcccggccag tacgtccgcc gcctgattct tcagatcttc
     7921 atcgattcgg gcgcgaacaa acgcgttagc agccattggg gtatctcctg tgctgtatat
     7981 ttattcagct tgaattgtag ctcaaatgag caacagcgac aatccatgag taatttaata
     8041 aacggatgtt tttacataat taactga
//
LOCUS       DADTFG010000184         8049 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.185, whole genome shotgun sequence.
ACCESSION   DADTFG010000184 DADTFG010000000
VERSION     DADTFG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.185"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            36..1202
                     /locus_tag="J1138_004168"
     CDS             36..1202
                     /locus_tag="J1138_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBA8455889.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFNRFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHIAVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1245..2561
                     /locus_tag="J1138_004169"
     CDS             1245..2561
                     /locus_tag="J1138_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8455890.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2665..3315
                     /gene="fabR"
                     /locus_tag="J1138_004170"
     CDS             2665..3315
                     /gene="fabR"
                     /locus_tag="J1138_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBA8455891.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTTIIPGNVKDE"
     gene            3315..3674
                     /locus_tag="J1138_004171"
     CDS             3315..3674
                     /locus_tag="J1138_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBA8455892.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(3714..4814)
                     /gene="trmA"
                     /locus_tag="J1138_004172"
     CDS             complement(3714..4814)
                     /gene="trmA"
                     /locus_tag="J1138_004172"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBA8455893.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4928..5118
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5183..7021
                     /gene="btuB"
                     /locus_tag="J1138_004173"
     CDS             5183..7021
                     /gene="btuB"
                     /locus_tag="J1138_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBA8455894.1"
                     /translation="MIKKASLLMACSVTAFSAWAQDTSPDTLVVTANRFQQPINTVLA
                     PTDIVTREDIERWQSRTVLDVMRRLPGVDVAQYGGMGQSSSLFIRGTESKHVLVLVDG
                     IPVARPGISNSSDIDQIPVSLVQRIEYIRGPRSAVYGSGAIGGVVNIITMTNKERSQI
                     NSSIGSKGYQQYDGTYRQRFGDTVVTAAGAYQTTKGFNVQPHSSYSGDGDRDGYRNKL
                     FWGGIEHKFNDQFDGYFRGYGYASNADYDQGNWGYDGGNDEHQNYTQSWDTGLRFNSG
                     VYSSQLVVNYQRIKDYNYSSLTGRYASGYTLDDMEQRYIQWGNNVIVGHGAISAGADW
                     KQEKLMSFGANGTDNYKRNNTGLYLTGQQQIDNITLEASGREDHDGQLGWHGTWQTAA
                     GWQFVDGYKVTLSYGTGFLAPSLGQQFGAERFGIASNPNLKPEESKQWEAGLEGLTGP
                     VDWRLSTYRYKIQNLIDYNNNTYFNVKSATIKGLEWTGNFTTGPIDHRLTLQYVDPRD
                     DETDQQLYRRAKQQVKYELSGEVYELGWDVTYQYIGERYDYDYDSTRKVKMGGLSVWD
                     IGLSYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            6966..7823
                     /gene="murI"
                     /locus_tag="J1138_004174"
     CDS             6966..7823
                     /gene="murI"
                     /locus_tag="J1138_004174"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBA8455895.1"
                     /translation="MATKLQDGNIPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         1872 a         1877 c         2189 g         2111 t
ORIGIN      
        1 attagcctgt ttataagtga ataaaggaat agccgatgat cgaacactat attcgtgggt
       61 ttgaggaaga acttcgcgag atccgccatc agatccacga aaacccggag ctgggattac
      121 aagaatttaa aaccagtgct ttggttgcgg aaaagctgcg ccagtggggc tatgaggttg
      181 agcaagggct ggcgacaacc ggcgtggtag ccacgttaaa agtgggcgac ggagagaaaa
      241 gtattggcct gcgggcggat atggatgcgt tgccgattta tgaaaatagt ggcaagccct
      301 gggccagtaa acatcctggt ttaatgcacg cctgcgggca tgacgggcat accaccatac
      361 tgctgggggc cgcgcgttat tttgccgaaa cccgccgttt taacggcacg ttacgtctta
      421 tcttccagcc tgcggaagag atgattaatg gcggtgagat catggttaaa gaggggcttt
      481 ttaatcgttt cccctgcgat gtcattttcg gcatgcataa catgcccggt ctgccggtgg
      541 gtaagtttta tttccagccc ggggcactga tggcgtcaat ggatcagttt catattgcgg
      601 ttcgtggttg tggcggacac ggtgcgatcc cgcacaaagc catcgatccg gtgctggttg
      661 ccgcacatat caccaccgca ttacaaagca ttgtgtcacg caatgtcgat ccgctggaag
      721 cggcggtaat taccgtcggc agcattgttg cgggtgaggc ggctaacgtc attcctgaca
      781 gtgctgagat gaaaatcagc gtccgctctc ttagccgcga tacccgacaa cttcttctga
      841 cccgtattcc tgctctggca caagcccagg ctgccagctt tggcgctacg gctgaggtta
      901 cgcatgttaa cggtacaccg gtattagtaa atgacgagga gatggcgcgc ttcgcctggc
      961 aggtggcgtg caaaacattt ggtgaagatc gggctgagtt tggcatcaaa cccctgatgg
     1021 gcagtgaaga tttctctttc atgctggaag cccaacccaa aggcggtttc ctgttattcg
     1081 gtaatggcga tgtcggggaa ggttcctgca tggtgcataa ccccggttac gactttaacg
     1141 atgcaagcct ggtcccggca agcagctact ggggcgcact ggttgaagcc tggctgcaat
     1201 aaataaaaca acacacaaca acactttttg gcaaggaaca tggaatgacg gcgatcgtaa
     1261 atgaaaccgt gagtgtgaag aaaaagccca gcggcaggcg ggtgatcttt gcctcggcgt
     1321 ttggcaatgc gctcgaattt tttgattttg gcgtttacaa cttttttgtg gtctatatca
     1381 gtacgctttt cttcccaccc agtgccgatc ataacgtcgc gctgttactg gcatttgcaa
     1441 cgtttggtgt gagctttttt atgcgtccgc tcggtgggat tatcgttggt gcctgggccg
     1501 atcgcttcgg gcgtaaaccg gcgatggtgt tcactattgc tctgatgagc ctcggtacgc
     1561 tgatgatcgg catcgcaccg acgtatgaaa cggcgggcta ttggggaact gcaacgctgg
     1621 tgctggcgcg tctaattcag ggcgttgcgg caggtggtga agtcggggct tcaatgtcgc
     1681 tactggtgga gtcagctccg gcaaatcgtc gcggctttta cagcagttgg tcactggcga
     1741 cccaagggct ggcaacgacc ttcggtgggg ttgttgcttt aggtctgagc gcatggctac
     1801 cgttcgccac aggttcagaa accgtaatgg cagaatgggg ctggcgtgtg ccgtttttta
     1861 ttggcgtgtt attagcacct atcggttgct ggttgcgtct gagcctggaa aatgacgtac
     1921 ctgagccagt gcgcaataaa aaagctgcta ccagcgaaag cgcgttttct ctgctcctgc
     1981 aacataaggc gactatcgtt aatggcgttt tactcgctat tggtagcacg gtcgcaacct
     2041 atatctccct cttttattac ggcacctggg cggcgaagta tctggcgatg cctcaacatt
     2101 attctcatgc agcgatgtta ctggcggggg taattacgtt tgtgggtgcg ctgttggtgg
     2161 ggatgctatg tgattcggtt gggcgtaaaa agctgatttt aatctcccgt gtgatggtgc
     2221 ttctctgtag ctggccgtca ttctggctgt tggtgaatta cccaagccct ggcatgttgc
     2281 tgacggtggt tttcgtgatg gtcagcttta ccacgcttgg cggtgtgcca gtgatgttgt
     2341 tgatctctga actgttgccg aaacggattc gggcattagg tttcgcgctg gtttatagca
     2401 ttggcgtagc aatctttggc ggttttgcgc aatatttcgc cacgcaatcc atcgtgttgc
     2461 tggatagtct gacggctccg gcgtggtatc tggggggagg cacgttgctg tcgatgctgg
     2521 cgttgttgta cgtaaaagaa ccggccaaag aattgcagta aatatgtttt attgcgttac
     2581 cgttcattca caacactgga gcaatccagt atgttcattc tctggtatag tgccagcagt
     2641 acttttggca aggattcaga catcgtgatg ggcgtaagag cgcaacaaaa agaaaaaacc
     2701 cgccgttcgc tggtggaagc cgcatttagc caattaagtg ctgaacgcag cttcgccagc
     2761 ctgagtttgc gtgaagtggc acgagaagca ggcattgctc ccacctcttt ttatcggcat
     2821 ttccgcgacg tagacgaact gggtctgacc atggttgatg agagcggttt aatgctacgc
     2881 caactcatgc gtcaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat ccgcacctcg
     2941 gtctccacat ttatggagtt catcggtaat aatcctaacg ccttccggtt attattgcgg
     3001 gaacgctccg gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat tcagcacttc
     3061 attgcggaac ttgcggacta tctggaactc gaaaaccata tgccgcgtgc gtttactgaa
     3121 gcgcaagccg aagcaatggt gacgattgtc ttcagtgcgg gggccgaggc gttggatgtc
     3181 ggcgtcgagc aacgccggca attagaagag cgactggtac tgcagctgcg aatgatttcg
     3241 aaaggggctt attactggta tcgccgtgaa caagagaaaa ccacaattat tccgggaaat
     3301 gtgaaggacg agtaatgaaa caagcaaatc aagatagagg tacgctgctg ctagcgttgg
     3361 ttgctggctt atcgatcaat ggtacttttg cagcgctgtt tagctccatt gtaccatttt
     3421 ctgtattccc gattatttcc ctggtgttga cagtttactg cctgcatcaa cgttatctta
     3481 atcgcactat gccggtaggc ttgccgggtc tggcagctgc ttgttttatt ctcggcgtac
     3541 tgctgtacag cacggtagtt cgtgcggaat atccggatat cggctctaac ttcttcccgg
     3601 cagtactgtc cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac cgtaagcagg
     3661 aagttgctga gtaatcggta tcatgccgga tgcaagcgca tccggcatgg gttttacttc
     3721 gcggtcagta atacgccgca ctccatatgg tgcgtgtagg ggaactgatc aaatagagcc
     3781 agacgttcga ccttgtgcgt ctggcttaat gtttccagat tcttgcataa cgtttccgga
     3841 ttgcaggaga tatacagaat acgtggatac gcctgcacca ttttctcggt ttcactgtcc
     3901 agaccgctgc gcggtgggtc gacaaaaatg gtttcgcact gataactctt taagtcgatt
     3961 ccttgcaggc ggttaaactc gcgcacacca ttcatcgcct gagtaaattc ttctgccgcc
     4021 atacgaataa tttgtacgtt atcaatatgg ttagcggcaa tgttgtattg cgcggcggcg
     4081 actgacggct tggcgatttc ggtggctaat acccgatcaa aattgcgtgc cagcgctaat
     4141 gaaaagttac cgttgccgca gtatagctcc agtaaatcgc cttttgagcc tttggttacg
     4201 tccagcgccc attccagcat ctgaatattc atcgccgcgt tcggttgggt aaagctgttt
     4261 tctacctgac ggtagatcat ctctttcccc gcgatcggca gacgttcgtc gatgtaatcc
     4321 tgatccagcg cgattttggt tttcgttgcc cgaccaatca gatgcacatt cagattctgc
     4381 gcgcgcagtg catcgcgcag ggcttccgcc tgctgacgcc actcatcatc cagcttttta
     4441 tggtacagca aggaaacaac cgcttgattg cttagcgtag tgaggtaatc aatctggaac
     4501 aacttgtggc gcagaatggg attattacgc acacccgcaa tcatcgccgt catcaactgg
     4561 ttgataagtt cactggcggc ggggaagcta tccacacgga tacgactttt ggtttgttga
     4621 tcgaaaatga tgtggtacag gtcatcgcca tcgtgccaga tgcggaactc cgcacgcatc
     4681 cgataatgac tgaccggcga gcgaaacact tccggaacca ggtcagaaaa cggtgccatc
     4741 atactttgca aacgtaccac tttttcggct aactgcgctt catactgttc tgttggaagg
     4801 tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt agggattgct
     4861 catcagatgt ccagatcttg atgaattcct atttgtgagc tacgtctgga cagtaacttg
     4921 ttacaacctg tagcatccac ttgccggtcc tgtgagttaa tagggaatcc agtgcgaatc
     4981 tggagctgac gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg acactgccat
     5041 ccggtgggaa gtcatcatct cttagtatct tagatacccc tccaagcccg aagacctgcc
     5101 ggccaacgtc gcatctggtt ctcatcatcg cgtaatattg atgaaacctg cggcatcctt
     5161 cttctattgt ggatgcttta caatgattaa aaaagcttcg ctgctgatgg cgtgttccgt
     5221 cacggcattt tccgcttggg cacaggatac cagcccggat actctcgtcg ttactgctaa
     5281 ccgttttcaa cagcctatca atactgttct ggctccgacg gatattgtca cgcgtgagga
     5341 tatcgaacgc tggcaatcca gaaccgttct ggatgtgatg cgccgcttac ctggtgttga
     5401 tgtcgcacag tatggcggca tgggacagag ttcttctctt tttatccggg gaaccgaatc
     5461 gaaacacgtt ctggtgttag ttgatggaat tccagtagca cgccctggta tatcgaatag
     5521 ctcagacatt gaccagatcc cagtctcgct ggtccagcgt attgaatata ttcgtggccc
     5581 acgctcggct gtttatggct ctggtgcaat tggtggtgtt gtcaacatca tcactatgac
     5641 gaataaagag cgttcgcaaa ttaactccag tatcggttcg aaaggctacc aacagtacga
     5701 tggcacatac cgccagcgtt ttggagatac ggtggtgact gctgcagggg cttatcagac
     5761 gaccaaaggt ttcaatgttc agccacactc aagttatagc ggagatggcg atcgcgatgg
     5821 ttatcgtaac aagctgttct gggggggaat tgagcacaaa tttaacgatc aatttgatgg
     5881 ttacttccgt ggctacggtt atgccagtaa tgccgattac gaccaaggga actggggata
     5941 tgatggcggc aatgatgagc atcaaaatta cacacagtca tgggatactg gattgcgttt
     6001 taattcggga gtctactctt cgcaactggt ggtaaattat cagcgcatta aggactacaa
     6061 ctacagcagt ctgactgggc gttacgcatc aggttataca cttgatgata tggagcaacg
     6121 ttacatccag tggggtaaca atgtcattgt tgggcatggt gctatcagcg caggagcaga
     6181 ctggaaacag gaaaaactga tgtcttttgg cgctaatggc acggataact acaagcgtaa
     6241 taacactggg ctttatctaa ccgggcagca gcaaattgat aacatcacac tagaagcctc
     6301 tggtcgtgag gatcacgatg gccagctagg ttggcatggc acttggcaaa cggcagcagg
     6361 gtggcagttt gttgatggtt acaaagtaac gctttcctat ggaactggtt tccttgctcc
     6421 ttcgcttggt caacaatttg gcgcagagcg gtttggtatt gcttctaacc ctaacttgaa
     6481 accagaagaa tctaagcaat gggaggcagg attagaaggc ttgacaggtc ctgttgactg
     6541 gcgattgtcg acctatcgct acaaaattca gaacctgatc gactacaaca acaataccta
     6601 ttttaacgtt aaatccgcga ccattaaggg cctggaatgg acgggtaatt tcacaactgg
     6661 tcctatcgat catcgtttaa cgttgcagta tgtagacccc cgtgatgatg aaactgatca
     6721 acaactctat cgccgtgcaa aacagcaggt aaaatatgag ttgagcggtg aggtttacga
     6781 gttggggtgg gatgtcacgt atcagtacat tggtgaacga tacgattacg actatgacag
     6841 tacccgtaag gtgaaaatgg ggggactgag cgtgtgggat atcggacttt catatccggt
     6901 cacctctcac ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attacgagac
     6961 agtctatggc taccaaactg caggacggga atataccttg tctggcagct acaccttctg
     7021 aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg
     7081 agatccggca tctcttgccg gatctccatt acatttatgc tttcgataac gtcgctttcc
     7141 cgtatggcga aaaaagcgaa gtgtttattg ttgagcgagt ggtggaaatt gtcaccgcag
     7201 tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacaccgcc agtaccgttt
     7261 cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta
     7321 aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaactg
     7381 ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa
     7441 tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctgcatggc gaagatgttt
     7501 ctctggatgc gctaaaacgt atcctacgcc cgtggttaag aatgaaagag ccgccagata
     7561 cagttgtatt gggttgcacc catttccctc tactacaaga agaactatta caagtgctgc
     7621 cagagggaac ccggctggtg gattctggcg cagcgattgc tcgccgaacg gcctggttgt
     7681 tagagcatga agccccggat gcaaaatctg ccgatgcgaa tattgcattt tgtatggcaa
     7741 tgacgccaga agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg
     7801 aaaaactggc agttttagat tgatttggtt gaatgttgcg cggttagaaa attattttaa
     7861 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
     7921 ggcaaacacg ccgccgggtc agcggggttc tcctgagaac ttcggcagag aaagcaaaaa
     7981 taaatgcttg actctgtggc gggaaagcgt attatgcaca ccccgcgccg ctgagaaaaa
     8041 gcgaagcgg
//
LOCUS       DADTFG010000185         7971 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.186, whole genome shotgun sequence.
ACCESSION   DADTFG010000185 DADTFG010000000
VERSION     DADTFG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.186"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            230..448
                     /locus_tag="J1138_004175"
     CDS             230..448
                     /locus_tag="J1138_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBA8455896.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKSKGGRQRDEAVGVEELCKQHKE"
     gene            complement(664..1593)
                     /gene="fdhE"
                     /locus_tag="J1138_004176"
     CDS             complement(664..1593)
                     /gene="fdhE"
                     /locus_tag="J1138_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBA8455897.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASTQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSIPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1590..2225)
                     /gene="fdoI"
                     /locus_tag="J1138_004177"
     CDS             complement(1590..2225)
                     /gene="fdoI"
                     /locus_tag="J1138_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA8455898.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2222..3124)
                     /gene="fdxH"
                     /locus_tag="J1138_004178"
     CDS             complement(2222..3124)
                     /gene="fdxH"
                     /locus_tag="J1138_004178"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HBA8455899.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3137..6187)
                     /gene="fdnG"
                     /locus_tag="J1138_004179"
     CDS             complement(3137..6187)
                     /gene="fdnG"
                     /locus_tag="J1138_004179"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5600..5602),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBA8455900.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPSSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6381..7214
                     /gene="fdhD"
                     /locus_tag="J1138_004180"
     CDS             6381..7214
                     /gene="fdhD"
                     /locus_tag="J1138_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBA8455901.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7302..7963
                     /locus_tag="J1138_004181"
                     /pseudo
     CDS             7302..7963
                     /locus_tag="J1138_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         1873 a         2044 c         2095 g         1959 t
ORIGIN      
        1 cataattcat ccttattatt tgttgtttct tttctgtaga tgtgcccccg cttaattaaa
       61 agtaataaac catttttgag taacaattga aataactgaa actgttacat tatctttgga
      121 tgccgtctcg caaaaacaaa acaatatgaa aaaatccgct ttcgttaatt aactttccgg
      181 cgtaattttc gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa
      241 tacggttttt cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggatg
      301 gcgtaaggtt tcgcgctctg caattttacg cgaagcggta gagcaatatc tggagcggca
      361 gcaatttccg gtgcgtaagt caaaaggcgg caggcaaagg gacgaggcag ttggtgttga
      421 agaactatgt aagcagcata aggaatgaca aaagtttttt tattcatgaa tataaaaaaa
      481 cagatgccct tattctggta ttaatacaag gctgttttac ttgaacttat aataactgca
      541 actgttacat catatctgga aaacgcctcg cgaaatacga gagattttgc gggaatagca
      601 ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg aaaacgtcag
      661 agattactcc ccttcacccg gaaacaggaa cgggttaatg gaactacggg catagccttc
      721 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
      781 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
      841 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgttcaca
      901 gttgctgcat tttacgcgca ctacgtgcca ttcagtttca cacaggttgc agtgcaggta
      961 acgcagaccc tgagtggtgc caatttgcac catgctggac accggtatag agccacatac
     1021 cgggcaatat tgacgttgtt cgccgtattc agcacgggct ttgccgggga tcagattagc
     1081 catctgcgcc caatagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1141 ggacgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct gagtcgatgc
     1201 cttctccaga ttctcaatca ctgccagcgc ggggccgctc atttcaggtt tcagttcagc
     1261 aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     1321 cgggggcttg ccttgtgtgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     1381 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     1441 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     1501 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     1561 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     1621 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtcac ccatccttcc
     1681 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     1741 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     1801 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag caacaggacc
     1861 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     1921 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     1981 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2041 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2101 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2161 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2221 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     2281 tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca aagccaacag
     2341 ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     2401 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac ataacatgtg
     2461 taccaccgac gcctgctggg tcgtacaaac ccgcattgtc gtaaccacgg gttttcagct
     2521 ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     2581 ccgttgggca ggtcttcacg caggccggtt cttgcccaac caccacgcgg tcaacgcaca
     2641 acgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     2701 ccgcaatgca ataaccgcag ccgatgcact gctcggactg gaagtcgacg ataccgttgg
     2761 catactgaat aattgcccct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     2821 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     2881 atcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac accccaatgt
     2941 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3001 agccgataca ggtggtaacg tcgatgagtt ttgccacttc ttcctggaag tcccgcgcct
     3061 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3121 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3181 tctgcgtgtt cgcatcaccg acgaatggcg tcaacgtatt ggcaataaag ccttttttcg
     3241 caacaccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgtttg
     3301 ctttcagcgt gcgaatacgt ttggtcacta ccgctttggc tttgatatag ccacggttgg
     3361 aggagacttt cacagtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     3421 tttccacaaa ctgctctggt tgcaaaatcg cattcaacag cgcgtgtttg gtccagtagt
     3481 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     3541 atgcttcggc gtcgtcttta aagatacgcg cagccgggtt cgagataacg tttggatgca
     3601 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     3661 ccatcttatc gagggcaaac agacgtccca tgccttcctg ctgcatgata aacggcccta
     3721 cgccgctgct cggaggcgct gcgctgtaat ccggaatatc ccagccggtc cacttagtgc
     3781 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     3841 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     3901 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     3961 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcagt tgggcgaacg
     4021 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccgggtcg gtaatatcgg
     4081 ccagcgcttt gccgttgctc tccattgcca cttcttctga agatggctca tgcggaatgg
     4141 cgtagttcca ggtcatgttc agcacctggt ccgggttcgc gccaccctgt tcggcataca
     4201 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccccg
     4261 gggcgtccgc acctttccag tgccattgca accagcgacc agagttaacg attgaaccgt
     4321 tctcttccgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     4381 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     4441 cgatagtcac gaggaatttc agcttcgaca gacagccgat cactttgttt ttgttcggga
     4501 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     4561 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     4621 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcattgag acgaagaatt
     4681 tcgggtagtt gccccagtag ttgacctggc cttccagcag tggttttggc gtgttggcag
     4741 cgaggtaagt ttgcagatcg gtctgcttct cgcttggcaa cgtcatgtaa cctggcagac
     4801 tctgcgacag tagccccagg tcggtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     4861 taacgccgcc gccagccatc cccatattgc cgagcagcaa ctggatcatc gccatcgtac
     4921 gaatgttttg cgcaccaatg gagtgttgcg tccagccgag cgcgtaaagg aacgaggcag
     4981 ttttatcgtg ggcactggtc tcagcgatgt attcgcagac tttcaggaat gcgtcttttg
     5041 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5101 agttccacac gcaacgtgga tgttgcaggg tagtatcgcg tttggcgaag ccgttttcgt
     5161 ccagttcgta agtccaggtg gacttatcgt acttgcgttt ttccgcgtcg tagccggtga
     5221 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     5281 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     5341 aagcaatgtc agtaccggaa cggatagggg catagtagtc agccaccgcc gccgtacgtg
     5401 taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct tccatcgccc
     5461 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     5521 cattcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     5581 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     5641 cgcgggagaa tttttgcgtt aaatagccgg tttcgttgct cgacgcggaa gcacacagca
     5701 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttcg
     5761 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     5821 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcaggcgg ctttcggagt
     5881 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc tccgcggttg accgggtgat
     5941 ccgggtcacc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6001 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6061 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6121 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6181 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6241 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     6301 tcaatttccc tccatccagg aaggatttgt aacagaatac cataatgttg gtgtgtgtgt
     6361 tcttatctgg ttaatagaaa gtgaaaaaaa cacagcaaaa agaaatcgag aatgtgacaa
     6421 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     6481 tagatgaggt ggcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcacgtgg
     6541 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgaag
     6601 gaattatcga aagcccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggcc
     6661 tggaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggaa cgccgccggg
     6721 cgctggcggg acgtacgggc tgtggcgtat gcggtgtaga acagcttaac gatatcggta
     6781 aaccggtgca gccgctgccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     6841 tacgtcatct caatgatttc cagccagtgg ggcaactgac tggttgtact cacgccgctg
     6901 catggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     6961 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7021 tgctggtttc cagccgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7081 agattttgtt tgcggtgtct gccgcgacca cacttgctgt agaagtggct gagcgctgta
     7141 atctgacgct ggtgggcttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7201 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattacagg acttattcat
     7261 ttcgtgaatt ttgttatttt ttcataaaca aaacgcgtgt agtggcgtta acgcctgcgc
     7321 tgcaacctat tgatggcgtg gcggtgagtt atattgatgc tgccgtagcg ttgggtaata
     7381 ccattaacga aatggataaa tattacaccc aggaaaatta taaagacgat gcgtttgcga
     7441 aagggaaaac gctgcaccag acattcttaa aaatctggaa gcctttgaac ctgtagcaga
     7501 atcttatcat gcggcgattc aggaaattaa tgacaagcgc cagcttacgg aattgaaaaa
     7561 tattgaagaa agagaaggga aaacattcca ctactactct ctggcagtca tgatttcagc
     7621 gaaacaaatt aataacctga tatcgcaaga taagtttgat gcagaagcgg caatgaagaa
     7681 agtgtctgaa ctggaaacgc tggtggcgca ggccaaagaa gcggataaaa gcggcatgaa
     7741 tttctcgttt attaattcgg caggccagta tcaactcgag gctaaaaaat acgttcgccg
     7801 catcagagat aaagtcccgt actctgactg ggataaagag caacttcagg acgcaaactc
     7861 aagctggatg gccgaagact catttccacg agcgttatgc gattacaacg aaatggtcga
     7921 tgaaatattt cagttaattg taatcgcggg ggcgggtttg tgatgagcac g
//
LOCUS       DADTFG010000186         7959 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.187, whole genome shotgun sequence.
ACCESSION   DADTFG010000186 DADTFG010000000
VERSION     DADTFG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.187"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            225..620
                     /gene="caiF"
                     /locus_tag="J1138_004182"
     CDS             225..620
                     /gene="caiF"
                     /locus_tag="J1138_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBA8455902.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(740..1330)
                     /gene="caiE"
                     /locus_tag="J1138_004183"
     CDS             complement(740..1330)
                     /gene="caiE"
                     /locus_tag="J1138_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBA8455903.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWNRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1336..2121)
                     /gene="caiD"
                     /locus_tag="J1138_004184"
     CDS             complement(1336..2121)
                     /gene="caiD"
                     /locus_tag="J1138_004184"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBA8455904.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2230..3783)
                     /gene="caiC"
                     /locus_tag="J1138_004185"
     CDS             complement(2230..3783)
                     /gene="caiC"
                     /locus_tag="J1138_004185"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBA8455905.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDQQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(3856..5073)
                     /gene="caiB"
                     /locus_tag="J1138_004186"
     CDS             complement(3856..5073)
                     /gene="caiB"
                     /locus_tag="J1138_004186"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBA8455906.1"
                     /translation="MDHLPMPKFGPLAGVRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            complement(5202..6344)
                     /gene="caiA"
                     /locus_tag="J1138_004187"
     CDS             complement(5202..6344)
                     /gene="caiA"
                     /locus_tag="J1138_004187"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBA8455907.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(6375..7889)
                     /gene="caiT"
                     /locus_tag="J1138_004188"
     CDS             complement(6375..7889)
                     /gene="caiT"
                     /locus_tag="J1138_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBA8455908.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLSWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
BASE COUNT         1918 a         2136 c         1984 g         1921 t
ORIGIN      
        1 aggcgcattt gaaaaacctc cggctatgcc ggaggatatt tatttgccca ttaagtaaat
       61 cttttgggga atcgatattt ttgatgacat aagcaggatt tagctcacac ttatcgacgg
      121 tgaagttgca tactatcgat atatccacaa ttttaatgtg gccttgttta attgctgcaa
      181 aacgagtcat agccagtctt ttaatttgtg aaactggagt tcgtatgtgt gaaggatgtg
      241 ttgaaaaacc actctatttg ttaatcgccg aatggatgat ggctgaaaac cggtgggtga
      301 tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt aataccctga
      361 cttatattct gtcggaagtc acagaaataa gctgcgaagt taagatgatc cctaataagc
      421 tggaagggcg gggatgccag tgccagcgac tggtaaaagt ggttgatatc gatgagcaaa
      481 tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga aagacgccgc
      541 gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag atgatgttgt
      601 caaagagtat gcgtcgttaa tgtcattccg ttgataccac gcagcctggt tgccggatgc
      661 agttcatggc gcatcttatc cggcctacgg ttgctttcca gccaggtcgg ataaggcgtt
      721 tacgccgcat ccggaaagat caccgtttcg gcgtcacatc cgtcgtcccc tgcaaacggg
      781 ggcgattttc ctccatttgc ctcagtggct gcgtttcatg taacgatgca tgacagcgcc
      841 cgacaagatc ctgatactct ttggtattca accggttcca gtgtaactcg tcgtcactca
      901 cgctgcgtac agcgcgggct ggcgtaccca tcaacaactg gcgtttctcg ccgcgaaagc
      961 ccgctttgac aaagctcata gcggcaacaa tgctctcttc gccaatgacc gcgccatcca
     1021 taatcacgct gttcatcccg accagtgcat cgcgaccaat cacacaacca tgcaggatcg
     1081 ctccgtgccc gatatgaccg ttttccccaa cgatggtgtc agtgtcgcag tagccatgca
     1141 taatgcagcc atcctgaata ttggctcccg cttgcacgat caaccgcccg tagtcaccac
     1201 gcagtgaggc gagtgggccg atgtagacac cggctcccac aatcacatcg ccaatcaata
     1261 ctgcactggg atggacaaac gccgtcgggt gaacaaccgg aattaacccc tcaaaggcgt
     1321 aatagctcac ggttgttaac gtcctttcca cactggatcg cgcttctcgg caaacgccag
     1381 cggcccttca atggcatcct ccgaatgcag aaccgatgga tagtgtttca acacgccgct
     1441 gcgaatatag cgatacgctt cttctaccgg catttcactg gtggtgcggt agatctcttt
     1501 cagcgccgca atcgccagcg gagcgctgtt aaccagctgc tgcgccagtt cgcgggcgtt
     1561 atccatcagt tccgcctggc taaccacgcg gttgactacc ccccaacgca gcgcctcttc
     1621 tgcgcccatt cgtctgccgg tcatcaccat ttcattgacg atggcaggcg gcaggatctt
     1681 cggcagacgc agcacgccgc cgctgtcagg aacgatgccc agtttggctt ccggtagggc
     1741 gaagctggcg ttatcggcac aaacaataaa atctgccgcc agcgccagtt caaagccgcc
     1801 gccaaaggca tagccgttca cagcggcgat gaccggtttg tcgagattga aaatttcggt
     1861 taatcccgca aaacctcccg ggccaaagtc agcatccggt gcttcgcctt ctgctgccgc
     1921 ttttaaatcc cagcccgcgg aaaagaattt ctctccggca ccggtaataa tggcgacacg
     1981 taattgcgga tcgtcacgga aatttagaaa tacttcgccc atttcaaagc tggtttttgc
     2041 atcaatagca ttcgcttttg gacgatcaag ggtaatttcc agaattgatc cattgcgggt
     2101 cagatgtaat gattcactca ttccttttct ccatttttgc tttttcaggg acgacaacat
     2161 ccctgcaaaa aatgcatatt gttttagagc gtgattatta gctgacaggg tagttccctg
     2221 ccgtttcatt tatttcagat tctttctaat tattttcccc gagcaattac gtggcagatc
     2281 ttttctgatc tccagataag agggcacttt aaatttcgcc atattctgtt cacagaagca
     2341 gaagaattcc tcttcgctca atgtttcacc ttcattcagc accacaaatg ctttgatggc
     2401 ttcatcgcga atcgaatctt taatacccac aaccacgata tcctgaattt ttggatgggt
     2461 ggcgatgata ttttcgagct ccacgcagga gacattctcg ccaccgcgtt tgatcatatt
     2521 gcagcggcga tcgacgaaat aaaaaaagcc ctcttcgtcg cggtatccgg tatcgccggt
     2581 atgtagccag ccatcggctt ccagcacttt cgcagtggct ttcgggttga gaaaatactc
     2641 tttgaagatg gttttccctg gtacgccttt aatacagatt tcaccgagct caccagccgg
     2701 gaggggacga ttgtgatcgt cgcggatctc cgcttcgtag caaaagcccg cccgaccaat
     2761 cgacggccag cgccgtttat cgccagggcg atcgccgata atgcccacaa tggtttccgt
     2821 catcccataa gacgtcagca agcgaacgcc gaagcgttca caaaacgcgt ctttttcctg
     2881 ctccgacaag ttgagataaa acatcacttc ccgcaggcgg tgttgctgat cgttcgctga
     2941 aggcggctgc accatcaacg tacggatcat catcggaata cattcggtaa tggtggcgcg
     3001 gtacttctgc acctgtcccc agaaggcacg ggcgctgtat ttctcgacca gcacaaaggt
     3061 ggccccggca gaaaacgccg ccatcgccgc agtacactgg caatcgatat gaaacgcagg
     3121 cattaccgtc aggtagacgt catcgtcacg cagtgcacac tgccaggcgg agtaatatcc
     3181 agcgaagcgc aggttgtaat gagttatcac cacccctttc ggtcgggagg tggtgccgga
     3241 ggtgaagaga atttccgccg tatcgtcagt cgatagcggc ggtgcatagc acaaggtggc
     3301 aggttgttga tttttcagtt gagtaaacga actcacgcca tcatcagcgg gaagtgccat
     3361 atctgtcagg caaatgtgcc gcaactgaga ggcatcttcc tgctgaatct gttgatacat
     3421 aggataaaat tgcgcactgg tcaccagcag gcacgcctgg ctattttgca ggatccacgt
     3481 gctttcttcg cgcaacaggc gggcgttaat cggcaccata atcgcgccaa tttttgccaa
     3541 cccgaaccag caaaagataa attccgggca gttgtcgaga tgtagagcaa ccttgtcgcc
     3601 tttgcgaatc cccagcgtat aaaacaggtt tgccgtgcgg ttaatctcct gatttaactc
     3661 aagataacta taccggttaa cgactccgct gctggattca caaatcagcg ccgttttatg
     3721 accgtaaacg tccgccagat cgtcccacat ttgacgtaga tgttgtccgc caatgatatc
     3781 cattgcacct ctatccattt tgttcgtttg ttattgggcg ggcgctagtc aggcaagttg
     3841 actgacgcca cgcgtttagt cctcaacttt ggccagacct ttgctgacca actcctgaat
     3901 gtcgttttcg ctgtagccga tatttttcaa aatggcagcc gtgtccatgc catgtgaggg
     3961 cattccgcgc cagatttgtc cggggttatt tttgaatttc ggcatgatat tcggcccttt
     4021 gcaggtgcga ccatccatcg tttgccactg agtgattgat tcgcgagcca catactgtgg
     4081 attgctttcc agttccggca cggtcagtac tttggcacag gcgatattca gttcggcaaa
     4141 gcgttcttta acttctgcga tggtatgtgc cgccagccag gcatcgagtt tctcttctac
     4201 cagtgggccg taagggcatt cgatacggtg gataagctga gtgccttccg ggatttccgg
     4261 cgtaccgagc aggtgtgcga ggccaatatc tttaaagcac tctgcaattt gagtaatgcc
     4321 caccagttcc atcacgatgt agccgtcggc gcatttatac agaccgcaac cggcgtagta
     4381 gggatcttta cctttggtca tgcgcgggca catttcgccg ccgttgaagt aatccatcat
     4441 gaagtactgg cccatacgca gcatcacttc atacatggcg atgtcgatgc tttcgccttt
     4501 gccggtttca cgcactttat gcagtgctgc aagcgccgcc gtggtggcgg tcaggccaga
     4561 aaagtaatcg gcggtatacg ggaaggcagg cattggctgg tcaacatcac cgttctgaat
     4621 caggtaacca ctgaaggcct gggcgatagt gttataggcc ggaagattgg tgtactcctc
     4681 ggtgccatac tgaccaaaac cggaaaggtg agcgataacc agttttgggt tgtgctgcca
     4741 caggacttca tcggtaatgc cacgacgggc aaaggccgga cctttactgg cttcgatgaa
     4801 gatatcggtg gtttccatta atttcagaaa cgcttcgcgc ccttcatctt tgaaaatatt
     4861 taacgacagc gcatgcaaat tgcggcggga gagctgcggg tagttcggtt gaacgcgaat
     4921 ggtgtcggcc caggcgacgt tctcgatcca gataacttcc gcaccccatt ctgcgaacat
     4981 ttgcccggca aacggtccgg cgatttcgat accggagaag acaacgcgca ctccggccaa
     5041 cggcccgaat ttcggcatgg gtagatgatc cattattagc tcctgaaaaa tttatgtagc
     5101 gcatgactgc cggatgcggc gtaaacgctt tatccggcct acattcgtgc tcccgtaggt
     5161 ctgataagac gcgtcagcgt cgcatcaggc agcgcacgga cttagcggta ttgcttcagc
     5221 accgcgcgac ccagcgtcag gatctgcatt tcgtcagatc ccccggaaac gcggtctaca
     5281 cgcaggtcac gccagaagcg gctgatgcgg tggttgcccg caatcccgac accgcccagc
     5341 acctgcattg cgctatccac aacttcaaat gccgcattgg cgcagaagta tttgcacatc
     5401 gctgcatcgc cagaggtgat ggtgccgttg tctgctttcc acgctgcttc atacagcatg
     5461 tttttcatgg agtttaattt gatcgccatg tgggcgaatt tttcctgaat caactggaaa
     5521 cgaccaatag cctcgccaaa ctgcacgcgc tggttggcgt agcgcgccgc atcttcaaag
     5581 gcgcacatcg ccgtaccgta gttggtcagg gctaccagga aacgttcatg gtcgaactct
     5641 tctttgacgc ggttaaagcc gttaccttcc cgaccgaaca tgtctttctc gtccagttcc
     5701 acgtcgtcaa aagtgatttc acagcagcta tccatacgca gaccgagctt ttcaagtttg
     5761 gtcactttga tgcccggttt gctcatatca acaaaccatt cggtgtagac aggtttgtcc
     5821 ggagaagccc cgtcgcgcgc catcaccacg atgtacgggg tgtaggcgct actggtaata
     5881 aaacacttac taccattaag ataaatctta ccatttctac gggtataagt cgttttcagg
     5941 ctacccacgt cggagcctgc gcccggttcg gtaatcgctg agttccacat ctgcttaccg
     6001 gtgccgcgga aagccatgat cttgtcgatc tgctcttgcg taccttcacg caggaaggtg
     6061 ttaaacccgc ccggtaactg atacagcacg taagttggcg ctcccagacg acccagttcc
     6121 atccacacgg cggcgagagt aacaaacccc gcgtccagac caccgtgttc ttccgggatc
     6181 agcagactgt cgatacccat atccgccagg gctttgacaa aacgttccgg gtagacgctg
     6241 tcacggtcgc actcggcaaa ataggcctcc cagttttcgc tggccatcag ttcgcggata
     6301 ccggcgacaa acagttcctg ctcatcattt aaattaaaat ccatctttca acctcttgat
     6361 attttggggg ttaattaatc tttccagttc tgtttcgcgt ctttaataaa ggagagcgtc
     6421 accatgatgt tgacgaagaa cagcgggcat cctccggcga taatggcggt ttgaatcggt
     6481 ttcaggccgc cgagcgccag cagaacaatg ccgataatgc caaccagaac tgaccaacca
     6541 atgcgcacta gcagcggggg ttcttcgccg tcacgcactt cgcggcaagt ggacatcgcc
     6601 agggtataag agcaggcgtt aaccagcgta acggtggcaa taaagcagag gatgaagaag
     6661 ccccacatgg tggcggtgct gagtggcaga gcggcccagg tttcgatgat ggcgcgcgcc
     6721 acaccgtact gttcgatcag atttggaatg ttgatgatgt ttttatctat caacagcaga
     6781 gtgttactac cgagtacagt ccacaggatc caggttgacg ctgtcagccc cagcaccatg
     6841 ccgaagcaca gttcacgcac ggtacgacca cgggagatgc gggcgaggaa gatactcatc
     6901 tggatagcgt aaatcaccca ccatgcccag tagaacacgg tccagccctg cgggaagccg
     6961 cctttagcga tgggatcggt atagaacaac atgcgcggca gatacatcag caacatcccc
     7021 accgaatcgg tgaagtagtt catgatgaag ctggcaccgc tgacgatgaa cacccaactc
     7081 agcatcagga agctcaggta actacgcacg tcactggcga tacgtacccc tttttgcaga
     7141 ccgcaagcga cgcaaatggc gttgaggata atccagcagg taatgatgat agcgtccagt
     7201 tgcagggtat gcggaatgcc aaacaaccat tgcatacact cggtcaccag cggcgtggca
     7261 aggcccagac tggtacccat cgcgaagatc aaggcgacga gatagaagtt gtcgacgata
     7321 gtgccgaaca accctttggc gtgtttttca cctaccagcg gcaccagggt ggagctgggg
     7381 cgaatcactt ccattttgcg gacaaagaag aagtaagcga aggcgactga aaggaagctg
     7441 taagtggccc acggcagagg tccccagtgg aacaagctgt aagccagccc caactctttc
     7501 gcgcctgttg agttcggttc taagccaaac ggcggggtgg agatgtagta gtagatctca
     7561 atcgatcccc agaacagtac ggcagcagac gtacaggagg cgaacatcat aaagatccaa
     7621 ctggcggtac taaattctgg tggttcgtta cctaaacgct ttttggcata cgggccaaac
     7681 accagccaga accagccgaa aagcatcacc accatatacc attcaaatgc ccatccccat
     7741 acattggtga cgtaactgaa tacagcatta ataacgacat tcgccgcatc cagatctctg
     7801 actgtaagcc aacaaagtat gccgacgatt attaacggcg gaaagaaaac cttcggttct
     7861 attcccgttt ttctcttttc attcttcatg agttaattcc actgtgaaaa cgaacattta
     7921 ttttgcgttc ccgtttgttt tatttttgtt aacatttaa
//
LOCUS       DADTFG010000187         7942 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.188, whole genome shotgun sequence.
ACCESSION   DADTFG010000187 DADTFG010000000
VERSION     DADTFG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.188"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(7..126)
                     /locus_tag="J1138_004189"
     CDS             complement(7..126)
                     /locus_tag="J1138_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455909.1"
                     /translation="MFTGSPLPLKAEGGVFRAQPESAHSKASAIKRFIKDFLS"
     gene            156..839
                     /locus_tag="J1138_004190"
     CDS             156..839
                     /locus_tag="J1138_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBA8455910.1"
                     /translation="MSEQKLIAESRTFEQQMIERDKRATKAGFVVGGVGLLIAVLALT
                     AVVVMLPLKQTDVELYTVDNHTGRVEHVTRTSKTSLTATEAYQKAMAANYVKVRERYI
                     YPSLQDDYETVQVYNSPQVNDDYLALYAGKDAPDKVYKNGAHAVRVEILSNQITDATA
                     PDRVATIRYRKIIRRLADNSTRNEYWDARFTFHSDPDKEMSDAEREINYFGFTVTSWQ
                     TDREIRGGE"
     gene            836..1744
                     /gene="virB9"
                     /locus_tag="J1138_004191"
     CDS             836..1744
                     /gene="virB9"
                     /locus_tag="J1138_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015874995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HBA8455911.1"
                     /translation="MMKKLTFTALALMMCGAAWGAAIPQASRYDSRVQQVIYNPQNVT
                     VVNTKPGFMTTLVFDNDEAVISAKPGFDEAWEATPDANRVNVRPVALTQGAPGEDGNT
                     TQVVIPPNSRDWHTNMLVVTSKRLYNVELNVIDDKSAQQPAFQVSWRYPGEERAKASR
                     EAMARQREWEQKQQQASIRKALNSAQTPRNWSYTKYPGTGSFNIVPDFAYDDGRFTFV
                     GFSPSKSIPSVTKELNGKEHVVNSSIQKKGDFTVLVIQEVTPRLVLRSGNTVVGLENS
                     GFGRVHATDGSTVSRQVERVEKPESR"
     gene            1788..3029
                     /locus_tag="J1138_004192"
     CDS             1788..3029
                     /locus_tag="J1138_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HBA8455912.1"
                     /translation="MNDENKEPVPDEAQASPAHAEDDIATLEREARAKREAELLSEQD
                     EEKDPVQPAVNKLKKRKRGKATAFLAITAVALIFLAWGGNWVYRNILWQPTEERKQDT
                     APQTNKTDYRQRNDLGMSTDTAEEEPEQQDNDQNNTGGTEQAAPAAPPELNKASFLIR
                     RDGSATTQNQVKTRQQEMTLASATTTGQQDSSAATNPAPSSPGQSPAPVRRIPYDPDL
                     YIPENTSIPCSLDRRFVSDRAGKLRCTVTTDIWSASGNTKLIEKGTTASLLYRAIAEE
                     GMKHGQGRAFIIATKLRTRQPPYLDIPLVDTSAAGELGEAGVDGWIDSHFWERFGGAL
                     MVGMIPDIGAWASNSAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGEI
                     INLITGEDIDFSNIYTLKIKQ"
     gene            3041..3361
                     /locus_tag="J1138_004193"
     CDS             3041..3361
                     /locus_tag="J1138_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455913.1"
                     /translation="MTRANKKRIAILVMYIARIVGQSLRDAPLALLIAGFLFLLFAPP
                     SAWMAINQIWYHLRPLTDREAIMLGKSVSGLLQLAWLVAVSSRFILSVDEVFSRMKEV
                     KDDR"
     gene            3351..4376
                     /gene="virB11"
                     /locus_tag="J1138_004194"
     CDS             3351..4376
                     /gene="virB11"
                     /locus_tag="J1138_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HBA8455914.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELYTKINGMW
                     EQHAVPLSYEDCYNFARCLAKHHGDNIEDIKPGLSATLESGERCQVIVPPACERDTVS
                     VTIRKPSKIQIPHQGYIDAGFYNRVTNDEKTETHDEELIALYNARNIPLFMEKCVEYG
                     KTLAVAGETGSGKTTFMKMLIQYIPVHLRITTIEDNPEIIFFKHRNYVHLFYPSESSD
                     EKGSVVTPASLLRWNYRMNPDRILLTEVRGAEAWDFLKITGSGHEGSMTSIHAGSAKE
                     AIDGFITRCYENPQCAQLPYTFMLRKVLDSLDVIVSIDLDGDVRRMNDIYFRPAHRNQ
                     YFEEMKA"
     gene            4373..4771
                     /locus_tag="J1138_004195"
     CDS             4373..4771
                     /locus_tag="J1138_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015874998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cag pathogenicity island protein Cag12"
                     /protein_id="HBA8455915.1"
                     /translation="MTNKMTVIILAAIITGCSSPPPPVPVAWDKAAEPLNTRLPQWRD
                     NNVTVPSPTVNGKWTLSVSTHSFHDTTWTPAVFYAAAHSTRIVVSAQSGTDFFNARNW
                     LRQNGAKGVIEYQPVFNCLTCRETTIYFSR"
     gene            4805..5107
                     /locus_tag="J1138_004196"
     CDS             4805..5107
                     /locus_tag="J1138_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kikA from plasmid origin"
                     /protein_id="HBA8455916.1"
                     /translation="MKKTVIAVAALCLAAGTPVYSFAADPCEVVLCMYGKATGNSGGR
                     ECRSAEKAFFKIVKKNKHGFLPNHTLKARKAFLGECPDADPEAVNKILSRYGKVRG"
     gene            5150..5455
                     /locus_tag="J1138_004197"
     CDS             5150..5455
                     /locus_tag="J1138_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dpoa decarboxylase"
                     /protein_id="HBA8455917.1"
                     /translation="MPKEDLFIRNIYSRNQDRISVALVYDTLSKEAHRGCGLYYEIYA
                     SRLIGLLRAHLSELNEADANKLMRYAESKGTKIDDTTWSEVLEAEMECRAEIYREQM"
     gene            6220..>7942
                     /locus_tag="J1138_004198"
     CDS             6220..>7942
                     /locus_tag="J1138_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017346477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HBA8455918.1"
                     /translation="MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNL
                     PREERKNAPFWLKSVACVSIFFFIFGTISPLTIQGLSYFFKLLDNNILFRIPLWIMTV
                     TFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNTRTDVRKVKELLPESIEYNPL
                     DYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILLYQAILAGE
                     AVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAG
                     FSLAKKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELE
                     EVALVNSINATNGFSLKEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRI
                     NSTPRTVAIFLDELKYHLSRPALEGLGTARDKGVHIFMAFQAIDDLRDCPSDLNGDAV
                     IGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKMDTDRMIR
                     QAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENPQSEQPQ
                     TEINEQNK"
BASE COUNT         2259 a         1953 c         1947 g         1783 t
ORIGIN      
        1 tctaattcaa ctgaggaaat ctttgatgaa acgcttgatt gcgctggctt tgctgtgcgc
       61 gctttccggc tgcgcccgga acacgcctcc ctctgccttc agcggtaagg gagaacccgt
      121 aaacacccct caaattatgg cggagttaaa cagtcatgag tgaacagaaa ttaatagctg
      181 aatcccgcac ctttgagcag caaatgattg agcgggataa acgcgccacg aaagccggct
      241 ttgttgttgg cggcgtgggt ctgctgattg cggtactggc gcttaccgcc gtggttgtaa
      301 tgttgccgct gaaacagacg gacgttgagc tgtataccgt ggacaaccac acaggacggg
      361 tggaacacgt aacccggaca tcgaaaacca gcctgacagc aacagaagcc tatcaaaagg
      421 cgatggccgc aaactacgtc aaagtgcgtg agcggtatat ctatccctct ctgcaggacg
      481 attacgagac ggtacaggtc tacaactccc ctcaggtaaa cgatgactat ctggcgcttt
      541 acgccggaaa agacgccccg gacaaggttt ataagaacgg ggcgcacgcc gtgagagtgg
      601 agatcctgtc caaccagatc accgatgcga cagcccccga cagggtcgcc accatccgct
      661 acaggaagat tatccgccgt ctggctgata acagcacccg caacgaatac tgggatgccc
      721 ggttcacgtt ccattctgac cctgacaagg aaatgagcga tgcggaacgc gagatcaact
      781 acttcggctt taccgttacc agctggcaga ccgaccgcga aatccgggga ggtgaatgat
      841 gaaaaagtta acgtttaccg cactggcact gatgatgtgc ggcgcggcat ggggtgcggc
      901 cataccacag gcaagccgct atgactcccg cgtgcagcag gttatctata acccgcagaa
      961 cgtcaccgtc gttaacacca aacccggctt tatgacaacg ctggtatttg ataacgatga
     1021 ggcagtcatc agtgccaaac ccggctttga tgaggcgtgg gaagccacgc cggatgcaaa
     1081 ccgggtcaac gttcgtccgg tcgcgctcac gcagggagcg cccggagaag atggcaacac
     1141 aacgcaggtc gtgatccccc ctaacagccg cgactggcat accaacatgc tggttgtcac
     1201 cagtaagcgc ctgtacaacg tcgagctgaa cgtcatcgat gataaatcgg cacagcaacc
     1261 cgctttccag gtgagctggc gctatccggg cgaagaacgg gccaaagcca gccgggaggc
     1321 gatggccaga cagcgcgaat gggagcagaa acagcagcag gccagtatcc ggaaagcact
     1381 gaactcggcg cagacgcccc gcaactggag ttacaccaaa tatccgggca caggcagctt
     1441 caacattgtt cctgattttg cttacgacga tggtcgcttt acgttcgtgg gcttctcccc
     1501 gtcgaagtcc atcccttccg tcaccaaaga gctgaacggc aaagagcatg tggttaacag
     1561 cagtatccag aagaaaggcg acttcaccgt actggtgatt caggaggtga cgccccgcct
     1621 ggtactgcgt tccggtaata ctgttgtggg tctggaaaac agcggatttg gcagggtaca
     1681 cgccaccgat ggttccaccg tatcccgaca ggtggaaaga gtggaaaaac cggaatccag
     1741 gtaacctata aaaatagttt tatattttta taaaaggaaa catgcatatg aacgacgaaa
     1801 acaaagagcc tgtgccggat gaagcgcagg catccccggc acacgctgag gatgatattg
     1861 ccacgcttga gcgggaagcc cgcgctaaaa gggaggccga acttctcagt gagcaggacg
     1921 aagagaaaga cccggtacaa cccgccgtaa acaagctgaa aaagcgaaaa cggggcaaag
     1981 caaccgcctt tcttgccatt actgcagtcg cgcttatctt tctggcatgg gggggtaact
     2041 gggtttatcg caatatcctg tggcagccca cggaagaaag gaaacaggac acagccccgc
     2101 agacgaacaa aaccgattac cggcagcgta acgacctcgg catgtccacc gatacagccg
     2161 aagaagagcc ggaacaacag gataacgacc agaacaacac ggggggaaca gaacaggcgg
     2221 ctccggccgc cccgccggaa ctcaacaaag ccagcttcct tattcgccgt gatggttcgg
     2281 ccaccacaca gaaccaggtc aaaacccggc agcaggaaat gacactggca tcggcgacaa
     2341 ccaccggaca acaggacagc agcgcagcca caaatcccgc cccatcgtca ccgggacaaa
     2401 gccccgcacc agtacggcgc ataccctatg accctgacct gtacatcccg gaaaacacct
     2461 cgatcccctg ttcactggat cgccgctttg tatctgaccg ggcggggaaa cttcgctgca
     2521 cggtcacgac tgacatctgg agcgccagcg gcaacacgaa gctgatagag aaaggcacca
     2581 cggcatcatt actttaccgc gccatagcgg aagaaggtat gaagcacggg caagggcggg
     2641 cattcatcat tgcgacgaag ctacgcaccc gccagccgcc ttatctcgat atcccgctgg
     2701 ttgataccag cgcggctggc gagctgggcg aggctggcgt tgatggctgg atagattcgc
     2761 acttctggga acggttcggc ggcgcgttaa tggtcggcat gatccccgat attggcgcat
     2821 gggcatccaa cagtgcgggt aaaaaagacc gcaatacgga ctatacggaa aacagccgcc
     2881 aggctatggc agatatggcg cgtaccacgc tggagaacag catcaatatt cccccaacgc
     2941 tctataagaa tcagggcgag atcatcaacc tgatcacagg cgaggatatt gatttttcaa
     3001 acatttacac actgaaaatc aaacaataaa ggaatacgtt atgacgagag caaacaagaa
     3061 acgcatagcc attctggtta tgtacattgc acgtattgtc ggccagagcc tgcgtgatgc
     3121 accacttgca ttacttatcg cgggttttct ttttctgctg tttgcaccgc cctcggcgtg
     3181 gatggccatc aatcaaatct ggtatcacct gcgcccttta actgaccgtg aagcgatcat
     3241 gttaggaaaa agtgtgtctg gcttactcca gcttgcctgg ctggtagcag tcagttctcg
     3301 cttcattctt tctgttgatg aagtcttttc ccggatgaaa gaggtaaaag atgaccgcta
     3361 aaaatatctc cctcgaccgc tataaacagc gtttctttgg cgactttctg gagcttcccg
     3421 gcctgacgga aatcgccgtc aaccgtccag gtgagctgta taccaaaatc aacggcatgt
     3481 gggaacagca tgccgttcct ttgagctatg aggattgcta caactttgcc cggtgcctgg
     3541 caaagcacca cggcgacaat atcgaagata ttaagccggg actgtcagcc acgctggaat
     3601 ctggcgaacg gtgccaggtt attgttcctc cggcctgtga gcgcgatacg gtgtctgtca
     3661 ccatccgtaa gccctcgaag atacaaatcc cgcaccaggg atatatcgat gcgggatttt
     3721 ataaccgggt aacaaacgac gagaagacgg agacacacga cgaagaatta attgcattgt
     3781 acaacgccag aaatattcct ctgtttatgg agaaatgcgt ggagtatgga aaaacattag
     3841 ccgtcgcagg tgaaactggt tcagggaaaa caacattcat gaaaatgttg atacagtata
     3901 tccctgtcca tttacgcata acgacgatag aagacaatcc ggagataata ttttttaaac
     3961 acaggaacta cgttcacctg ttttatcctt ctgagtcatc cgatgaaaaa gggtctgtcg
     4021 ttactccggc gagcctttta cgctggaatt accgcatgaa ccctgaccgc attctgttga
     4081 cagaagttcg cggcgcggaa gcctgggatt ttctcaaaat tacaggttcc ggccatgaag
     4141 gaagcatgac ctccattcat gccgggtcag caaaagaagc aatagatgga tttattaccc
     4201 gttgctacga gaacccacag tgcgcccagc tcccctatac atttatgttg cgtaaggttc
     4261 tggacagtct ggacgtgata gtgagcattg atttggacgg agatgtccgc cgtatgaatg
     4321 acatttattt ccgcccggcc catcgcaatc aatattttga ggagatgaaa gcatgacaaa
     4381 taaaatgaca gtcatcatcc tggcggcaat aattaccgga tgttcatcgc cgccgcctcc
     4441 cgttcctgta gcgtgggata aagccgctga gccattaaac acccgtttac cgcaatggcg
     4501 tgacaataac gttacagtcc cgtccccgac agttaacggt aaatggacat tatcagtcag
     4561 cacacatagt ttccacgaca caacatggac gccagcagta ttttacgctg ccgcccactc
     4621 aacacgaatt gtcgtctctg cacaatccgg tactgatttc tttaatgcca gaaactggct
     4681 tcgccagaat ggcgcaaaag gcgtcattga ataccagcct gtttttaatt gcctgacgtg
     4741 cagggaaacc accatttatt tctctcgcta atctgtttaa aacacaatct gataaggatt
     4801 taccatgaaa aaaaccgtta tcgcggtggc cgcactatgt ctggcagcag gaaccccagt
     4861 ttattccttt gccgctgacc cgtgcgaagt tgttctttgt atgtacggaa aagctacggg
     4921 taatagtggc gggcgtgaat gccgttctgc agaaaaggct ttttttaaaa tagtcaaaaa
     4981 gaataaacat ggtttcttac ccaaccatac tttgaaggcg agaaaagcgt tccttggtga
     5041 atgtcccgac gcagatccgg aggccgtaaa taaaatactg tccaggtacg gtaaggtcag
     5101 aggatagtaa taatccgtat aacatcctgt ttactgtaaa aggtgcgaca tgccaaaaga
     5161 agacttattc atccgaaata tatactcccg caatcaggac agaatatcgg tagccctggt
     5221 atatgacacg ttatcaaaag aagcccacag aggttgcggg ctgtattacg aaatatacgc
     5281 aagcagatta atcggccttt tgcgcgctca tctttcagaa cttaatgaag ctgacgcaaa
     5341 caaattaatg aggtatgcag aaagtaaagg cacaaaaatt gatgatacaa cctggagtga
     5401 ggtgctggaa gcggaaatgg aatgcagagc tgaaatatac cgcgaacaga tgtaatcaca
     5461 cagaacaaaa aatgtattgt acaacacaca ggctaacagg cgacggggtt ttaaggcttc
     5521 attatttttg gcttcaattt cgaagaaatt gttaagacga aaaataatga agtggaacgt
     5581 ggacagaaaa ttcaatttcg gagaaattgc taattttcag gctccacgtc cacccgcgaa
     5641 cattcgcccc agcgaattga agcgggccgg ggggagccgc aaaaattttg ccgccccagc
     5701 gagcaaaaag gcacgcccac gtgccggttt ttgtgggggg cttgccccgc acaccgtcgc
     5761 agccaggcta tatttcccgc cccagcgggg aatatgccga ttaggcgcga ccaacccctt
     5821 taaagcagcg ttcccatttt ttcgagcttg cgaagaaaaa ataggctaaa cgcgcgtctt
     5881 aaaggggttg gtcgcgcgta gcgcgcgacg gtgtgccgcc tttgaccctg ggggttttgg
     5941 tccggcgtcc agccgggaca ttacccccat gtgccatgca gtgagggcgt ccagcccgaa
     6001 cgtaatgaca cacgccgttg cccgttattc gggcgtttca gaggacagac gaccactggt
     6061 cggctgtttt ctgaaagcag ggataacggg cttgatcgcc actggcgatc actgtctttt
     6121 tctgttttat ggagtcatcg catggagtca agctgcgact ccatgcgatg actccatgac
     6181 ttgaccccat cactacgatt tttaatggag ttagcaaata tgggatcaca tcttatgcaa
     6241 aggctgaatg cttttgctga cgccttttcc tttttcctgt tatggctgca aaacagccct
     6301 gtcatattat ccctgttcgc aggtcttaca ttaccgttta tagtcaacct tccccgagag
     6361 gaaagaaaga acgctccgtt ctggttaaaa tcggtggcgt gtgtgtctat tttctttttt
     6421 atattcggta ccatatcacc gctaactatt caggggctga gctacttttt taaattactc
     6481 gacaacaaca tactgttcag aattccacta tggatcatga cagtcacatt tacgacagca
     6541 ggattattct ttcatattgc agcccgacga cttctggcgg gagaaattga caacctgaaa
     6601 catcgaatga ttaagaaaac caagctggag agaaatacca gaacggatgt acgtaaagta
     6661 aaagagctgt tgcccgagag catagaatac aacccactcg attacattga tttaaagaaa
     6721 ggtgctttca ttggcttaaa caaaaatgac cagccgcaat acataaccat aaaagaattc
     6781 aagacccaac acgcagcaat tatcggcaca actggttcag ggaaaagtgt cactgcagcc
     6841 attctacttt atcaggcaat tcttgcaggc gaggctgttt ttgtagaaga tccaaaagat
     6901 gatgaatggg caccacatgt actcagggaa gcatgtaaaa aagcgggcaa gaaatttaca
     6961 cttataaatt tgaacaagcc aaattttcag cttgatttac tggctgatat ctctcatgaa
     7021 caactggagg aactatttaa cgctggcttt agtctggcta aaaaagggga agcttcagat
     7081 ttttatcgaa tctctgaccg ccgcgcagcc agaaatacat cagcaattta tgaaaaagga
     7141 atgacacttt atgatttatt taacactgat tttgttcagt cattacgtca agctgctccc
     7201 gcattttttg gtgagctgga agaagttgct ttagttaact caattaatgc cacaaatgga
     7261 ttttcattaa aggaggtttt tgatgaaggt ggttgttgct acattattgg ttcaacacga
     7321 aatcaaaaaa taatatccgc acaaaggatg attctgaccc gcctgataca aatagcagag
     7381 accagggaca gaataaacag caccccgcgt actgttgcta tttttctcga cgaattgaaa
     7441 tatcatctat cccgccccgc ccttgagggg cttggaacgg cacgagataa aggcgtgcat
     7501 atattcatgg cttttcaggc gatagacgat ctgcgtgact gtccgtctga tttaaatggt
     7561 gatgcagtta ttggagccat cattgaaaac gccaaattca aattgattta taaaattcaa
     7621 aatccagaaa cagcagaatg ggcggcaaaa atgacaggtt caatcctggt cgatgatgaa
     7681 atgaggaaag taaggactga tttatcatta acggaaaaaa tggatacaga ccgtatgata
     7741 agacaggcag aaagttacta cgttgacagt aatatgtttt taaatctccc tgaaaaggtc
     7801 ggctttgtat ttacaacaaa agaactggcg aaggcaacaa agatgtcccc tattctggtt
     7861 aaaaagaaac atattgaatt gctgtcattt gaaaatcctc aatctgaaca accacaaacc
     7921 gaaatcaatg aacaaaataa gc
//
LOCUS       DADTFG010000188         7858 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.189, whole genome shotgun sequence.
ACCESSION   DADTFG010000188 DADTFG010000000
VERSION     DADTFG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.189"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            152..880
                     /gene="gadW"
                     /locus_tag="J1138_004199"
     CDS             152..880
                     /gene="gadW"
                     /locus_tag="J1138_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBA8455919.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1243..4356)
                     /locus_tag="J1138_004200"
     CDS             complement(1243..4356)
                     /locus_tag="J1138_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBA8455920.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4381..5538)
                     /gene="mdtE"
                     /locus_tag="J1138_004201"
     CDS             complement(4381..5538)
                     /gene="mdtE"
                     /locus_tag="J1138_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBA8455921.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5598..5927
                     /locus_tag="J1138_004202"
     CDS             5598..5927
                     /locus_tag="J1138_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455922.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASM
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(5877..6404)
                     /gene="gadE"
                     /locus_tag="J1138_004203"
     CDS             complement(5877..6404)
                     /gene="gadE"
                     /locus_tag="J1138_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBA8455923.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7203..7775)
                     /gene="hdeD"
                     /locus_tag="J1138_004204"
     CDS             complement(7203..7775)
                     /gene="hdeD"
                     /locus_tag="J1138_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBA8455924.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT         2080 a         1877 c         1839 g         2062 t
ORIGIN      
        1 tattatcaat taactgttat gcaaaactac tttgtggata aaatttggtc ctaccaaata
       61 tggcagtttt tgcacacaga aacagtctgg cgtcatttca ttagtatact gacattgaaa
      121 taatcgcagt aatgaaatat aagggatagt catggctcat gtctgctcgg tgatcctcgt
      181 tcgtcgttca ttcgatattc atcatgaaca gcaaaaaata tcgttgcata acgagagtat
      241 cctgctgctg gataaaaatt tggcagacga ttttgcgttt tgttcactgg atacgcgacg
      301 gctggatatc gaagagctga cagtttgcca ttacttacaa aatattcgtc agttgccacg
      361 caatttagga ttgcatagca aagaccgttt gttaattaac cagtcacccc ccatacagct
      421 ggtgacggcg atttttgata gtttcaatga cccccgggtc aattcgccga tactgagcaa
      481 aatgctctat ctttcctgtt tatcaatgtt ttctcataag aaagaactga tccccttact
      541 tttcaatagt atcagtactg tttcaggaaa agttgaacgc cttattagct ttgatatcgc
      601 taaacgttgg tatctacgcg atatcgcaga aagaatgtac accagcgaga gtctcatcaa
      661 aaaaaagttg caggatgaaa atacctgttt cagtaaaata ttactcgcct ccaggatgtc
      721 gatggccaga cgattactcg agttacgtca aatacctctg catactattg cggaaaaatg
      781 tggctatagc agtacgtcat actttataaa tacatttaga caatattatg gtgtaacgcc
      841 acatcagttt tcgcaacatt cgccgggtac cttttcctga catatttcgc atttgaatat
      901 tggtcaggat ctcacgtctg cttcatgtga aactctttcc tgatgatttc tgccgggcta
      961 ccggctagtt ctctttcgca gtttgccagg cggtataggt attttcagga ggaaaagact
     1021 cggcatgttt gggattatta agctaacaat tcatacgatt acgggaatgt gggtcagtat
     1081 tgtgcttttc aaattgatga cgaatggctg gagtggtttc tactttcagt gctgtgtact
     1141 aagcctggta ttcctgacgg ttagctggtt gttgagtggt gaatggttag caggaaagag
     1201 taaggctgaa ccctcacgtt caaccttact ctcatttaca cgttacgctt ttttaaagcg
     1261 ggcaaagaga tgttccacta caacgaaaaa gaccggaacg aagtaaattg ccagcactgt
     1321 tgcggcaaac atcccgccca ttacgccggt acctaccgcg ttttgcgcgc cagagccggc
     1381 accatgactg ataaccagcg gcaggacgcc gagaataaag gccagagaag tcatcaggat
     1441 tgggcgtaaa cgcatacgcg ctgcttcgat gattgcctct accggcgttt tcccttcttt
     1501 ctgcatcatc tcaacggcaa attcgacaat caggatggcg ttcttggcgg aaagcccgat
     1561 ggtggtcagc aaaccaacct ggaagtaaac gtcattactt aagccgcgca gatcggtggc
     1621 cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc accgagaacg gaattgacca
     1681 gctttcataa agtgcggcga gggcgaggaa caccacgacc agtgaaatgg catatagcgc
     1741 gggagcctga tttgaggata acgcttcctg atacgacagt ccggtccatg agtagccgac
     1801 gcctgccgga agttttgcga ccaggtctgc cataaatttc atggcgtcac cggtactttt
     1861 cccggcagct gcttcaccta aaatctccat tgacgggatg ccgttgtagc gttccagtcg
     1921 cggtgaacca taggtccatt cggtagacga ataggcagaa agcggtgcca tcgtgccaga
     1981 ggcgttgcgt acataccatt ggttgatgtt atccggcaac atacggaacg gcgtgcctgc
     2041 ctggacatac acttttttca cccgcccctg gttgaggaag tcgttcacgt agctgctacc
     2101 gaaggcggtg gaaattgtct ggttgatatc agacagcgcc acacccatag cctcggcttt
     2161 cgcagcgttg acgttcactt tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc
     2221 gatgacctga ttcggtgatt gcgctgccag tgataacagc tcgtttcgcg cctgggtcag
     2281 cttttcgtgc cccagattgc cgttgtccag cagttccata tcaaaacctg acgcggtacc
     2341 cagttcagcc accgcgggta agttgaacgg gaagacgacg gctttattga tactgcttag
     2401 cgcaatcatt gcccgctgaa taatcgcggt aaccgagttt tcctcgccga cacgttcaga
     2461 ccacggcttg aggctgataa acgccagacc gttgttttgc ccctgaccac tgaagccaaa
     2521 gccaccaacg gtaaacaccg actgaacatt atttttctct tttgtcagat aataatccgt
     2581 cacctgttgt agcactttcg tggtgttaac catggttgca ccggaaggta actgcgcggt
     2641 ggtcataaat accccctgat cctcttctgg taagaaagag gtcggcgtac gcaggaacag
     2701 caccgccatc ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa
     2761 cagcgagcgg gtgctgtcgg tatagtgctg agttgatttt tcaaacagcg tgttgaagcg
     2821 tgcgaacagg gcgttaggtt tgtgaccgcc ctccggcgcg gctttcagaa tggtggcgca
     2881 cagggcaggg gtcaggctca ttgctacaaa tactgaaagc agcatggagg agatcagcgt
     2941 gatggagaac tggcggtaga tctccccggt cgcaccgctc ataaaggcca tcggcataaa
     3001 cactgcggaa agaacaacgg caatcccgac cagcgcacgt tggatctgcc ccatcgattt
     3061 atgcgtcgct tccttcggcg gtagcttatc ttccgcaatg acacgctcga cgttctccac
     3121 caccacgatg gcgtcatcca ccagtaaccc tatcgccagc accatcccga acatagttaa
     3181 cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg
     3241 tacggcaatc gtcgggatga ttgtggcgcg gaaattctgc aaaaacagat acatgaccag
     3301 gaagacgagg atgatagcct caaccagcgt tttgaaaact tcctgaatag aaattttgat
     3361 aaacggtgtg gtgtcgtaag gataaaccgt cttcaggctt gcagggaaat aagccgataa
     3421 acggttcagc tcctctttca ccgctcgcga ggtatccagg gcgtttgctc cggtagccag
     3481 tttgatggcg atcccggcag caggtttgcc gttatagcgc gccacggtgg aataatcttc
     3541 cgccccaagt tcgacgcggg cgacatcacg cagcagcact tgcgaaccat cttgctgaac
     3601 tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca caatgatcga
     3661 ggcgttcagt tgctggtctg ccgcctgtgg catgccaccc agttgaccac cggaaatctg
     3721 gttgttttgc accttaatct gggaaataac atcggaaggt accaggttat atttattgag
     3781 tttttgcggg tccagccaga tacgcatggc gtactcggaa ccaaagagct gtacgctacc
     3841 aacgcccgcg gtacggctta gcgggtcttt gatattagac gctacatagt ccgcgatatc
     3901 gtactggttg aggctgccgt tatcagaaat aaatgccgct accatcagga tattactgct
     3961 cgacttatcg acgctaattc cctgctgctg caccgcttca ggtaatgaag gcatagcgag
     4021 ttgcagttta ttttgcacct gaacctgtgc gatatcaggc gatgtccccg tctcgaaggt
     4081 cagagtgatg gaggcattac ctgccgcatc gctggttgaa gacatgtaca tcaggccatc
     4141 aagcccattc atattttgct caatcacctg agtgaccgag tcttctaccg tttgcgcatc
     4201 ggcacccgga taggttgcgc tgacggtaat ggtcggtggc gcaatctgcg gatactgcgc
     4261 aaccggtaag ttcatgatcg ccagaccgcc tgcaagcatc ataataatgg caagtaccca
     4321 ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa gtccccttaa gcctgcaacg
     4381 ttattgtttc gattcggtgc tggcgttttc ctggctggag gaaattgctc gtgctttgat
     4441 acccggacga atgcgttgca agccggaaac gatcacccga tcgccagcct gcaagccaga
     4501 ggtgacaacc cactggtcgc cgatggcttt gctggcttcg atttcgcgta gctgcacgac
     4561 atcgtcttta tccagaatga gtgccgttgc tttaccctgg gcgttgtggg tgacgccttc
     4621 ctgcggcacc agtaatacat tctggcggct accttcatcc actaatgccg tgacatacat
     4681 gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt
     4741 ggtttcatcc accgtcgggt cggagaattt cagcgtgccg gtctggccgt aacgtttacc
     4801 attttccaga ttgagctgta ctggcgtact gccctgaacc tgtttgattt gcccactggc
     4861 gacctcttct ttcatgcgca agaaatcctg caccgactgc gtgagatcga cataaatcgg
     4921 gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac
     4981 ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag gtgacattcg cgtattgcag
     5041 attgatcgtt gcctgttcaa cagccgcttt ggcgacggtg acattggctt ctgcttcatt
     5101 caactgggtg cgcgcggtgt cataatcctg acggctaaca tagttggtct tcagcaacga
     5161 tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta gagagcgctt tcgccagcga
     5221 gcctttggcg gagttcagct ctgcctgtaa aggcgcagga tcaatctgat acagcgaatc
     5281 gccctggttc actttatcgc cttcgataaa gttgcgttta atgataatac cgcccacctg
     5341 gggacgtatc tcggcaactt cataaggaac ggttctaccg ggcaattcgc tcaacacatt
     5401 gaccgaaccg ggggagagtg tgacgacacc gacctcaggc gtcatggcgg cggcgttttc
     5461 cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag aataacaacg gtattaacag
     5521 ttttcttctt ctgttcattt tagtccctga aaattcttga gaaataacgg ttctttaaat
     5581 tccgcgcgag gagagatatg agggatttac agaccagcgg catagtgggg ttgtcagcca
     5641 gtaagatggg atgcaagcac agtgatcgac atggtgaggt caacgacaaa aaaaatgttg
     5701 ccgttctgcc aacagtttct gccagcatgc gggcaacgaa gggttctact ggtggataca
     5761 cataccaggg gaataaagtc gttttcgtct ctccttcaat aatcaccggg ggtattgaaa
     5821 tcagcatctg catccctcgt catgccagcc atcaatttca gttgcttatg tccttactaa
     5881 aaataagatg tgatacccag ggtgacgatg tcgctcatac gtcttaattt cagcttgtac
     5941 ataatgttga atttctgaat ctttaatgtt tgttgcgaaa tgttgagcgt tgacgtgata
     6001 ttgctttcat ttttgttttt cagaatcagt tgcataattt tgaactgatt atgtgaaaaa
     6061 catagttgtg aattcttatg gggcaagtgt ttaccataag taatctcaag aagatttttg
     6121 atactttctt tacggctgac aaaataaata ggcgctttag cttttagttt actgatgtga
     6181 taaggtgcca aaacaataat tctctcggca tctaatttct ccaggaattt aatcgcttct
     6241 tcatcaagga tatggttgtg ataggtgtca atgatcacct tgaagggttt attgcctact
     6301 tttttgatct ctgacaggga attgattttt atcatttcgt gattatcttt caactgccaa
     6361 aagccctgta aaagaaaaga atctttcgtc atgagaaaaa tcataacttg ctccttagcc
     6421 gttatcgttt cgaacatgtc atccttttca ttatttacat ccttgtccga atcgttgttc
     6481 aatatagtaa acgcctataa gaactttgcc cttcgtgaac taatacgtct attaacacta
     6541 atcaatactt cagatttatc catcacacac tccatatatt ggtcctaagt aagattcctg
     6601 gttgttatca gtttgtaact agtaaacaaa aggttaactt ttgcggatta ctgcccaaga
     6661 ataagttatc aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat
     6721 ccgatttaaa tatcgagtct ccttgtttcg acttaggctg gcaattggat tgccagcttt
     6781 cttttaacgc attgaaatta cctgattaaa taactcattc atgtcttcag gtttttatat
     6841 agcgtctgga gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac
     6901 ccggattact tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc
     6961 ccacagcact tttaaaacat taaacctgac cacggaaaat atcagccatg ataatgtttg
     7021 aaatggctaa tttgccatgg aacgaaaaaa atcagattaa attcagtagg ttgaaataat
     7081 caacagcagg caattgtttc aatgatagtg cgtaattgaa ctgaaacact gtatagctaa
     7141 gagagtatta actggatcat agtcacttaa tgtgactatg atctaggtgg aaatagggat
     7201 aattattgct ttttaacgaa caaactggcg aagctgaaca gactggcggc gctaaatatc
     7261 agttcaattc ccaccagtgt ggaaaccagc gttaccgaca ccatcggcgt agcaccaagg
     7321 aatatccagg caatgacgat atccagcaca ccaataacga gctgtagcca actgcctttc
     7381 attgaacgct gacgatacca actcatcagg cgaataaccc ctgccacaca gaacaaaccg
     7441 gcaataaatg ccgcaatggc aaaaatgccc agctctggtg cgcggatgaa gaaatagccg
     7501 atcaataaat aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg
     7561 cggttgctga atagcccgac aataagcgca atgcccgagc agattaataa tgcacccact
     7621 actgtgctta aaatgtcgcc agagacgaac ggaaaactga tacacagcaa cccgacgata
     7681 aacagcagca cggcaataaa ctggattgct ctgcgatgtt ttttaagcat ctccagatca
     7741 aacttcaaaa ttgttgcctt atctatatat aacatagaac caccctataa aattaagaag
     7801 aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa gaatgcagtc gatttaat
//
LOCUS       DADTFG010000189         7797 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.190, whole genome shotgun sequence.
ACCESSION   DADTFG010000189 DADTFG010000000
VERSION     DADTFG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.190"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            225..3026
                     /gene="sucA"
                     /locus_tag="J1138_004205"
     CDS             225..3026
                     /gene="sucA"
                     /locus_tag="J1138_004205"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBA8455925.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            3041..4258
                     /gene="odhB"
                     /locus_tag="J1138_004206"
     CDS             3041..4258
                     /gene="odhB"
                     /locus_tag="J1138_004206"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBA8455926.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            4352..5518
                     /gene="sucC"
                     /locus_tag="J1138_004207"
     CDS             4352..5518
                     /gene="sucC"
                     /locus_tag="J1138_004207"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBA8455927.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            5518..6387
                     /gene="sucD"
                     /locus_tag="J1138_004208"
     CDS             5518..6387
                     /gene="sucD"
                     /locus_tag="J1138_004208"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBA8455928.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            6627..7742
                     /locus_tag="J1138_004209"
     CDS             6627..7742
                     /locus_tag="J1138_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBA8455929.1"
                     /translation="MGVNSDVYAADVNIDILSATVKDKRIEGVSVTLQRNGAQSVSGT
                     TNASGSVNLGSTFADDQDALLIVKKEGYSNLVVKCSCAGMTYAISPAMTSLDGMRVVL
                     SWGEKPFDLDSHLLFPGGHIYFDSKEGTDANLDVDDTDSYGPETVTISKKHFGESYIY
                     AVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIWTVFKLNPNGEFE
                     DINSVTSANFNDTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQLARKYNREGEAVY
                     KTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWANRKAISLASGANA
                     ATTRANSYYNIAKIYETAGQDADALQHYQLALHDAIL"
BASE COUNT         1957 a         1968 c         2123 g         1749 t
ORIGIN      
        1 cagcgccgga tgcggcgtga atgccttatc cggcctacaa aagaaatgca ggaaatcttt
       61 aaaaactgcc cctgacacta agacagtttt taaaggttcc ttagcggacg cagaaaacac
      121 gacaagtccg caacaagtga accccggcac gcacatcaga tatgaaatgt gcgtggtagt
      181 atccacggcg aagtaagcat aaaaaagatg cttaagggat cacgatgcag aacagcgctt
      241 tgaaagcctg gttggactct tcttacctct ctggcgcaaa ccagagctgg atagaacagc
      301 tctatgaaga cttcttaacc gatcctgact cggttgacgc taactggcgt tcgacgttcc
      361 agcagttacc tggtacggga gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt
      421 tccgccgcct ggcgaaagac gcttcacgtt actcttcaac gatctccgac cctgacacca
      481 atgtgaagca ggttaaagtc ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc
      541 atgcgaatct cgatccgctg ggactgtggc agcaagataa agtggccgat ctggatccgt
      601 ctttccacga tctgaccgaa gcagacttcc aggagacctt caacgtcggt tcatttgcca
      661 gcggcaaaga aaccatgaaa ctcggcgaac tgctggaagc actcaagcaa acctactgcg
      721 gcccgattgg tgccgagtat atgcacatta ccagcactga agaaaaacgc tggatccaac
      781 agcgtattga gtctggtcgc gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg
      841 aactgaccgc cgctgaaggc cttgaacgtt accttggcgc aaaattccct ggcgcaaaac
      901 gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct taaagagatg atccgccacg
      961 ctggcaacag cggcacacgc gaagtggttc tcgggatggc gcaccgtggt cgtctgaacg
     1021 tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt cgacgagttt gccggtaaac
     1081 ataaagaaca cctcggcacg ggcgacgtga aataccacat gggcttctcg tctgacttcc
     1141 agaccgatgg cggcctggtg cacctggcgc tggcgtttaa cccgtctcac cttgagattg
     1201 taagcccggt cgttatcggt tctgttcgtg cccgtctgga cagacttgat gagccgagca
     1261 gcaacaaagt gctgccaatc accattcatg gtgacgccgc agtgaccggg cagggcgtag
     1321 ttcaggaaac cctgaacatg tcgaaagcgc gtggttatga agttggcggt acggtacgta
     1381 tcgttatcaa caaccaggtt ggcttcacca cctctaaccc gctggatgcc cgttcgacgc
     1441 cgtactgtac tgatatcggt aagatggttc aggcaccgat tttccacgtt aatgcggatg
     1501 atccggaagc cgttgccttt gttacccgtc tggcgctcga tttccgtaac acctttaaac
     1561 gtgatgtctt catcgacctg gtatgctacc gccgtcacgg ccacaacgaa gccgacgagc
     1621 cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa acatccgacg ccgcgcaaaa
     1681 tctacgctga caagctggag caggaaaaag tcgcgacgct ggaagatgcc accgagatgg
     1741 ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt tgtagcagag tggcgtccga
     1801 tgaacatgca ctctttcacc tggtcgccgt acctcaacca cgaatgggac gaagagtacc
     1861 cgaacaaagt tgagatgaag cgcctgcagg aactggctaa acgcatcagc acggtgccgg
     1921 aagcagttga aatgcagtct cgcgttgcca agatttatgg cgatcgccag gcgatggcag
     1981 ccggtgagaa actgttcgac tggggcggcg cggaaaacct cgcttacgcc acgttggttg
     2041 acgaaggcat tccggttcgc ctgtcgggtg aagactccgg tcgcggtacc ttcttccacc
     2101 gccacgcggt gatccacaac cagtctaacg gttccactta cacgccgctg caacatatcc
     2161 ataacggcca gggcgcgttc cgcgtctggg actctgtact gtctgaagaa gccgtactgg
     2221 cgtttgaata cggttatgcc accgcagaac cacgcaccct gaccatctgg gaagcacagt
     2281 tcggtgactt cgccaacggt gcacaggtgg ttatcgacca gttcatctcc tctggcgaac
     2341 agaaatgggg ccggatgtgt ggcctggtga tgttgctgcc gcacggttac gaagggcagg
     2401 ggccggagca ctcctccgcg cgtctggaac gttatctgca actttgcgct gagcaaaaca
     2461 tgcaggtttg cgtaccttct accccggcac aggtttacca catgctgcgt cgtcaggcgt
     2521 tgcgcgggat gcgtcgtcca ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc
     2581 tggcggtatc cagcctcgaa gaactggcga acggcacctt cctgccagcc atcggtgaaa
     2641 tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat gtgttctggt aaggtttatt
     2701 acgacctgct ggaacaacgt cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg
     2761 agcaactcta cccgttcccg cataaagcga tgcaggaagt gttgcagcag tttgctcacg
     2821 tcaaggattt tgtctggtgc caggaagagc cgctcaacca gggcgcatgg tactgcagcc
     2881 agcatcattt ccgtgaagtg attccgtttg gggcttctct gcgttatgca ggccgcccag
     2941 cctccgcctc tccggcggta gggtatatgt ccgttcacca gaaacagcaa caagatctgg
     3001 ttaatgacgc gctgaacgtc gaataaataa aggatacaca atgagtagcg tagatattct
     3061 ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc
     3121 cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc gaaactgaca aagtggtact
     3181 ggaagtaccg gcatcagcag acggcattct ggatgcggtt ctggaagatg aaggtacaac
     3241 ggtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc aacagcgcgg gtaaagaaac
     3301 cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa cgccagcagg cgtctctgga
     3361 agagcaaaac aacgatgcgt taagcccggc gatccgtcgc ctgctggctg aacataacct
     3421 cgacgccagc gctattaaag gcacgggtgt gggtggtcgt ctgacccgtg aagatgtgga
     3481 aaaacatctg gcgaaagccc cggcgaaaga gtctgctccg gcagcgcctg ctccggcggc
     3541 gcaaccggcc ctggctgcac gtagcgaaaa acgtgtgccg atgactcgcc tgcgtaagcg
     3601 tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga
     3661 agtcaacatg aagccgatta tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg
     3721 ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa
     3781 acgttacccg gaagtgaatg cgtctatcga cggtgatgat gtggtctacc acaactattt
     3841 cgacgtcagt atggcggttt ctacgccgcg cggtctggtg acgccggttc tgcgtgatgt
     3901 cgataccctc ggcatggcag acatcgagaa gaaaatcaaa gagctggctg ttaaaggccg
     3961 tgacggcaag ctgacggttg aagatctgac cggtggtaac ttcaccatca ccaacggtgg
     4021 tgtgttcggt tccctgatgt caacgcctat catcaaccca ccgcagagcg caattctggg
     4081 tatgcacgct atcaaagatc gtccgatggc agtgaatggt caggttgaga tcctgccgat
     4141 gatgtacctg gcgctgtcct acgatcaccg tttgatcgat ggtcgcgaat ccgtgggctt
     4201 ccttgtaacg atcaaagagt tgctggaaga tccgacgcgt ctgctgctgg acgtgtagta
     4261 gtttaagctt cacctgcact gtagaccgac gtggataggg tgtcggtcta cggtttaaaa
     4321 gataacgatt actgaaggat ggacagaaca catgaactta catgaatatc aggcaaaaca
     4381 actttttgcc cgctatggct taccagcacc ggtgggttat gcctgtacta ctccgcgcga
     4441 agcagaagaa gccgcttcaa aaatcggtgc cggtccgtgg gtagtgaaat gtcaggttca
     4501 cgctggtggc cgcggtaaag cgggcggtgt gaaagttgta aacagcaaag aagacatccg
     4561 tgcttttgca gaaaactggc tgggcaagcg tctggtaacg tatcaaacag atgccaatgg
     4621 ccaaccggtt aaccagattc tggttgaagc agcgaccgat atcgctaaag agctgtatct
     4681 cggtgccgtt gttgaccgta gttcccgtcg cgtggtcttt atggcctcca ccgaaggcgg
     4741 cgtggaaatt gaaaaagtgg cggaagaaac tccgcacctg atccacaaag ttgcgcttga
     4801 tccgctgact ggcccgatgc cgtatcaggg acgcgaactg gcgtttaaac tgggtctgga
     4861 aggtaaactg gttcagcagt tcaccaaaat cttcatgggt ctggcgacca ttttcctgga
     4921 gcgcgacctg gcgctgatcg aaatcaaccc gctggttatt accaaacagg gcgatctgat
     4981 ttgcctcgac ggcaaactgg gcgctgatgg caacgcactg ttccgccagc ctgatctgcg
     5041 tgaaatgcgt gaccagtcgc aggaagatcc gcgtgaagca caggctgcac agtgggaact
     5101 gaactacgtt gcgctggacg gtaacatcgg ttgtatggtt aacggcgcag gtctggcaat
     5161 gggtacgatg gacatcgtta aactgcacgg cggcgaaccg gctaacttcc tggacgttgg
     5221 cggcggcgca accaaagaac gtgtaaccga agcgttcaaa atcatcctct ctgacgacaa
     5281 agtgaaagct gttctggtta acatcttcgg cggtatcgtt cgttgcgacc tgatcgctga
     5341 cggtatcatc ggcgcggtag cagaagtggg tgttaacgta ccggtcgtgg tacgtctgga
     5401 aggtaacaac gccgaactcg gtgcgaagaa actggctgac agcggcctga atattattgc
     5461 agcaaaaggt ctgacggatg cagctcagca ggttgttgcc gcagtggagg ggaaataatg
     5521 tccattttaa ttgataaaaa caccaaggtt atctgccagg gctttaccgg tagccagggg
     5581 actttccact cagaacaggc tattgcatac ggcactaaaa tggttggcgg cgtaacccca
     5641 ggtaaaggcg gcaccaccca cctcggcctg ccggtgttca acaccgtgcg tgaagctgtt
     5701 gctgctactg gcgctaccgc ttctgttatc tacgtaccag caccgttctg caaagactcc
     5761 attctggaag ccatcgatgc aggcatcaaa ctgattatca ccatcactga aggcatcccg
     5821 acgctggata tgctgaccgt gaaagtgaaa ctggatgaag caggcgttcg tatgatcggc
     5881 ccgaactgcc caggcgttat caccccgggg gaatgcaaaa tcggtatcca gccgggtcac
     5941 attcacaaac caggtaaagt gggtatcgtt tcccgttccg gtacgctgac ctatgaagcg
     6001 gttaaacaga ccacggatta cggtttcggt cagtcgacct gtgttggtat cggcggcgac
     6061 ccgatccctg gctctaactt tatcgatatc ctcgaaatgt tcgaaaaaga tccgcagacc
     6121 gaagcgatcg tgatgatcgg tgagatcggc ggtagcgctg aagaagaagc agcggcgtac
     6181 atcaaggaac acgttaccaa gccagttgtg ggttacatcg ctggtgtgac tgcgccgaaa
     6241 ggcaaacgta tgggtcacgc aggtgcgatc attgccggtg gtaaagggac tgcggatgag
     6301 aaattcgctg ctctggaagc cgcaggcgtg aaaaccgttc gcagcctggc ggatatcggt
     6361 gaagcactga aaactgttct gaaataaata tctgtaataa gaaatagccc tcgccgcttc
     6421 cccctacagg aagggcgaag ggctgtcggt ttcgacatgg ttggccatct tatgatggcc
     6481 ttttttattc gggcactaat ctgaaaagtt tgcccactgc ttttctccca tcctctgcgc
     6541 aataatgccc ggcaaaatat cccctctccg ttctgtaaaa gcatttatac aagaagaaaa
     6601 ttgttctgcc ggttgctctg gccctgatgg gtgtaaattc ggacgtttat gcggccgatg
     6661 taaatatcga tattttaagt gcgacagtga aagataaacg cattgaggga gtatcggtga
     6721 cgctgcaacg caatggcgca caatctgttt ctggaaccac aaatgcatcg ggcagcgtta
     6781 atctgggttc tacatttgct gatgatcaag atgctttatt gatcgtcaaa aaagagggct
     6841 attctaatct ggtggtcaaa tgttcgtgtg ctggcatgac ttatgctatc agtccggcaa
     6901 tgactagtct ggatggcatg cgtgtagttt tgagctgggg cgaaaagcct tttgatcttg
     6961 attcacattt gctcttccct ggtggacata tctattttga tagtaaagag gggactgatg
     7021 ccaatctgga tgttgatgat acggatagct atggaccaga aactgtcact atcagcaaaa
     7081 agcattttgg tgaaagttac atctatgccg tgcaggacta tagcaataaa gggctgccca
     7141 acagtaatta tctctctgcc agtaaggcaa aagtgtttgt ttatgtaggg agctccctgg
     7201 tacgtagtta tagtgtccct gctggaaaac gagggaacat ctggaccgtg tttaaactca
     7261 atccaaacgg cgagtttgaa gatatcaact ctgttaccag tgccaatttt aacgacacca
     7321 cattagacgt cagggattta gccactgtga taatgcctgc gacggactct tcagctccgg
     7381 cttcgcctgc gatgcaaaac agtggagata ctcaactggc aagaaaatat aatcgcgaag
     7441 gtgaagctgt ttataaaacc ggtcaacttg agcaagccat tcaactcttc caacaggcaa
     7501 cagaattaga cggcaattac ggacaggcat ttagtaacct ggggctggcg tatcagaaaa
     7561 atggcaacat cgcggaagca atttgggcaa atcgtaaagc gatctcgtta gccagtggag
     7621 caaatgcggc gacaacgcgc gctaactcct attataacat tgccaaaatt tatgaaactg
     7681 ctggacagga cgctgatgca ttgcagcatt atcaactcgc tcttcacgat gccattttat
     7741 aaaaatgaaa gtggaatcat attttttaat tttatatgct tgcttgtttt tctggtc
//
LOCUS       DADTFG010000190         7606 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.191, whole genome shotgun sequence.
ACCESSION   DADTFG010000190 DADTFG010000000
VERSION     DADTFG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.191"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            33..197
                     /locus_tag="J1138_004210"
     CDS             33..197
                     /locus_tag="J1138_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455930.1"
                     /translation="MRKFSEQIFKITGDENVAKNELEPWTPEGTDPNYCWWEVDPVDA
                     ANEAMSYYND"
     gene            233..862
                     /locus_tag="J1138_004211"
     CDS             233..862
                     /locus_tag="J1138_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455931.1"
                     /translation="MAELTKEWLQNMITGIESVIDDKSFVCDEIVFKIGEVKNILTAF
                     KVTLASLEAEPVAEIRAHYPLGINGGKQKFVQAIGDLPDFGGHLYAAPPAPVVPDEGR
                     DQFEALIRFHVGDENHETLLLRANEGMNYRDPNVDFAWIFWKSSREHLLRNNDGSLPE
                     SGHQVRGLTLLVKRLASSLKSVNKSSKLPDKAMDYLKRNGLISVEDVLR"
     gene            complement(1067..1366)
                     /locus_tag="J1138_004212"
     CDS             complement(1067..1366)
                     /locus_tag="J1138_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA8455932.1"
                     /translation="MTFEVIITPEAEQQIINLLRYITEKAGSVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGIYHGGQSYEADFL"
     gene            complement(1372..1629)
                     /locus_tag="J1138_004213"
     CDS             complement(1372..1629)
                     /locus_tag="J1138_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HBA8455933.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRTAIRSGK"
     gene            1765..2043
                     /locus_tag="J1138_004214"
     CDS             1765..2043
                     /locus_tag="J1138_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455934.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFEKALSLDRRQWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2045..3094
                     /locus_tag="J1138_004215"
     CDS             2045..3094
                     /locus_tag="J1138_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBA8455935.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYSTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPESVLRRSLGLPVEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIVLPHNAHQQQNPPQEKPVVSVTVDPESPESFMKRPKRRRWVNEKYTLWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLAFEEKYGSQVDL
                     IFRFLDHAFATGVLG"
     gene            3107..3466
                     /locus_tag="J1138_004216"
     CDS             3107..3466
                     /locus_tag="J1138_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBA8455936.1"
                     /translation="MRTEFVLPWPPTVNTYWHRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLEIKITEVGSA"
     gene            3463..4128
                     /locus_tag="J1138_004217"
     CDS             3463..4128
                     /locus_tag="J1138_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBA8455937.1"
                     /translation="MNNQYLQFVREQLMIATADLSGSTKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEHQIAITRWAWNEFNAQSGARKIAGKTLVRLKTLIWLASQAVKAE
                     LFGGEGYGYQELALLTGVTTKNWSKTFTGHWVTMKHIFHRLDSETLLLVVRTRSTQKA
                     AFS"
     gene            4382..5095
                     /locus_tag="J1138_004218"
     CDS             4382..5095
                     /locus_tag="J1138_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455938.1"
                     /translation="MDDSALLRNSSLFVAYMGCLGWGSAYFYGWGTSFYYGFPWWVVG
                     AGVDDVARSLFYAVTVIVIFLIGWGVGIVFFLGIKQKRNIQNLSFIRLFLAILLLFIP
                     PVLEFSVIHQHVEPDVLIFCVIAVFIITLFVRSGRRLVSVSCFSEMYFIRHHRIEFMM
                     AGFMIYFWAFSLIAGWYKPQFKREYQAIHYENVWYYIIARYDDRLVLSKSYSSGGKKF
                     VICNSGNIDDFEINVVRVR"
     gene            5270..5467
                     /locus_tag="J1138_004219"
     CDS             5270..5467
                     /locus_tag="J1138_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBA8455939.1"
                     /translation="MQKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRHLPQ"
     gene            5618..6676
                     /locus_tag="J1138_004220"
     CDS             5618..6676
                     /locus_tag="J1138_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBA8455940.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     SWDNQWAGDEDYLKWLDQCLAQFWRVLKPTGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSTSQWQLPNERDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVAAYTSLNRQYAELQCEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLRQIICASSRPGDMVADFFMGSGSIIKAAMALGRHAIGVELEA
                     ERFEQTAREVLNLISNGH"
     gene            6718..6793
                     /locus_tag="J1138_004221"
     tRNA            6718..6793
                     /locus_tag="J1138_004221"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6751..6753,aa:Ile,seq:cat)
     gene            6796..6872
                     /locus_tag="J1138_004222"
     tRNA            6796..6872
                     /locus_tag="J1138_004222"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6830..6832,aa:Lys,seq:ttt)
BASE COUNT         1931 a         1644 c         2034 g         1997 t
ORIGIN      
        1 aaaccattaa cgcggaaaaa tataacgagt gggtgagaaa attctctgag cagattttta
       61 aaattactgg cgacgagaat gtggcaaaaa atgaattaga gccgtggaca cctgaaggaa
      121 ccgacccaaa ttattgttgg tgggaggttg atccggttga tgctgcaaat gaagccatga
      181 gttactacaa cgattaatgc cggaagacta cccgaaagag aggtaatgaa aaatggctga
      241 attaaccaaa gaatggctac agaacatgat taccggaatt gagtcggtaa tagacgataa
      301 atcttttgta tgtgatgaaa tagtattcaa aatcggtgag gttaaaaaca tacttaccgc
      361 atttaaagtc acgctggcat cgctggaagc agagccggtg gctgagatta gagcgcacta
      421 cccattaggg attaacggcg gtaaacaaaa gttcgtccag gccattggag atcttcctga
      481 ctttggcgga catctgtacg ccgctccgcc agcgcctgta gtgccggatg aggggcgcga
      541 ccagtttgaa gcacttatca ggtttcatgt cggtgatgaa aaccatgaaa cattattgct
      601 gcgggccaac gaagggatga attaccggga cccaaatgtt gattttgcat ggatattctg
      661 gaagtccagt cgtgagcatc tgcttcggaa taatgacggc agtctgccag aatcgggtca
      721 tcaggtacgc ggattaacac tgctggttaa gcgactggcc agttcattaa aaagcgtcaa
      781 taaatccagc aagctgcctg ataaggcgat ggactacctg aagcgaaacg gactgataag
      841 cgtggaggat gttttgcgat gacctggcct gaagcattca caacggtagg gattgcaatg
      901 gcggtggcgc tggtggtgta ttcgatttgc cgctggggat aaatcaccga aaaaagatcc
      961 cgacgcatac acgagccggg atctttgatt tatatagcct acgaatccgc cagtaagaga
     1021 gggggcggac ggtttatttt aacaccggaa tgatgtgggt aaaagtttat aagaaatcgg
     1081 cttcatagct ttgcccacca tgataaatac ccgcgataaa gactttattg ccatcaacgg
     1141 caaaagcaat aatcgttctg tggcggaagt gagttacccg cattccctgg cgaatatcat
     1201 cgcgtttatt gccccgatgc gggaatgtag aaaacccatc aagataatca agaagcgcat
     1261 tggcataatt gtcagcaatg acgcttcctg ctttctccgt gatatatctg agcaggttta
     1321 ttatctgctg ttcggcctcg ggggtaatga tgacttcaaa tgtcatgcag attacttccc
     1381 ggatcgaatc gcggtgcgaa cctgtgaaat ggagcgtccg ttgtttgggt tttcgcggat
     1441 agagtcaaga gagggggcgg ctgaatgcgt taaccacgct tcgattgctt tatcgcgctc
     1501 attcagtgcg cgaagccctt cacgaatgac ctcactttct gaagcatagg caccggaagc
     1561 cacacgggcg cgcaccatgt cagccatctc gttagttaat gtaatgctga attgttgggt
     1621 tgtacgcatg gtaaacctca cggagtagga tagaacacta ttcgatgata gcacgctgcc
     1681 tgttgacgac aacagaaatc agaggcaata ttgccgcgcg ccagcctgaa caactggcac
     1741 ctgctgcgtc agcagagaaa actgatggcg cacgatacca gattttacaa ttcggataac
     1801 tctgccgccc ctgccagcag gcacgggcgg cgttctcatg cattcaaatc tgactggttc
     1861 cagcatgacc catgcactga agaacaggcc gaatggttga tacagtgcta ccgcagacgc
     1921 gggtatgagt ttgagaaagc cctcagcctt gaccgccgcc aatggataat ctctgtaagg
     1981 ctcccttatt ccgaacgccc accgcgtccg tcccgtacat tccagcaacg catctggagg
     2041 taacgtgcgg gtattacttc gacctgttct ggttcctgaa ctcgggctgg tggtcgttaa
     2101 gccgggccgt gagtccatgc cggtattcca caatacccgg gtactggtgg agccggaacc
     2161 gaaaagcatg cgtaatctgc cgtccggggt cgttcctgcc gttcgccagc cgctggtgga
     2221 agacaaaaca ttgctgccgt ttttcagtaa cgcacgggtg attcgtgctg ctggtggtgc
     2281 tggtgcattg tctgactggc tgttgcgcca tattaaatcc tgccagtggc cacacggcga
     2341 ttatcatcac agcgaaaccg tcattcaccg ttatagtact ggcgcgatgg tgttgtgctg
     2401 gcactgcgac aaccagctgc gcgaccagac ctccgaatca ctcgagcaac ttgctcacca
     2461 aaacttgtca gcatggatga ttgacgtcat tcgtcacgca atgaatggca cacaggagcg
     2521 ggaattatcg ctggctgaat tatcctggtg ggcggtctgc aatcaggtgg ctgatgcgct
     2581 tccggagtct gtattgcgtc gttcgctggg attaccggtg gaaaaaatcc gctccgtata
     2641 cagtgagagc gacatcatac cgggagagca gactgccacc agcattctga agcagcgcac
     2701 taaaaatatt gtgctgccgc ataacgccca ccagcaacag aacccaccac aggaaaagcc
     2761 ggtggtcagc gttaccgttg atccggagtc tccggaatct ttcatgaagc ggcctaaacg
     2821 tcgccgttgg gtaaatgaga aatacacgct ctgggtaaag acacagccgt gtgcatgttg
     2881 tggtaagcca gctgacgatc cgcatcacct gattggtcat ggtcagggag gaatgggaac
     2941 aaaggcccac gatattttca cgctaccgct gtgccgggaa catcacaacg aacttcatgc
     3001 agatccgctg gcgttcgaag aaaagtatgg ttcccaggtt gatttgattt ttcgttttct
     3061 tgatcacgct tttgcaactg gcgtgcttgg gtgaaagagg tgactgatgc gtacagagtt
     3121 tgttttgccc tggccgccga cggtgaacac ttactggcat cgtcgtggca gcacatattt
     3181 tatatctgag gagggaaagc gctatcgccg ggctgtggcg cttattgttc gccagcagcg
     3241 gttgaaacta agcctgtccg gaaggctggc gataaaggtg attgcagagc ctccggataa
     3301 gcgacgtcgg gatctggaca atatcctgaa agcgcccctg gatgcgctga cgcatgcggg
     3361 gctgttaatg gacgatgagc agtttgatga aatcaatatt gtacgtggtc agccagtatc
     3421 tggtggacgc ctggagataa aaattactga ggtggggagt gcatgaataa ccagtattta
     3481 cagtttgttc gtgagcaact catgattgcc actgcggatc tcagtggttc gacaaaaggc
     3541 cagctggaag cctggcagga gaatgccctg ttcgatacag ggcgttacag acgcaaaaaa
     3601 atccggtacc gcgatgaagt aaccggaaaa atgataacgc gggataatcc accgatcccg
     3661 ggaaaacaat cgctggcgaa aggctcatca attgccctgg tcagtccggt tgagttttca
     3721 acatcatcat ggcggcgggc agttctgtct cttgaagaac atcataaagc ctggctgctg
     3781 tggtgttaca gcggtagcat ttgctgggag catcagatcg cgataacgcg gtgggcgtgg
     3841 aatgaattta atgctcaatc cggtgccaga aaaattgcag gaaaaacgct ggtgcgcctg
     3901 aagacgttga tctggttggc gtcgcaggcg gtaaaagctg agctttttgg tggggaaggt
     3961 tatggatatc aggaactggc gttactgacg ggggtaacaa ccaaaaactg gtccaagaca
     4021 tttactggtc actgggttac aatgaaacac atttttcatc ggctggatag tgaaacttta
     4081 ttgttagtgg tgagaacgcg ttcaacacaa aaggcagcgt tttcatagca aagtattgca
     4141 aaagtagatt aaaaggcata tgttttgtgt aaatctgata ttctgccgat tttctacgtg
     4201 atggcaaagc tagtaaaacc cgcgaccgag cgggttttta tctcctaaaa aaacagtatc
     4261 gacattaaac gttatgacga ttgtgccaat actttctcct tctatgacgc cccttgactg
     4321 tatggaatcc aatttgttat gtaatgcgtg ttaatatttt tgagttgtta atggtgttac
     4381 tatggatgac agtgctctgc tcagaaactc ttcacttttt gttgcttata tgggctgtct
     4441 cggatgggga agcgcttatt tctatggatg gggtacttca ttttactatg gctttccatg
     4501 gtgggttgtc ggggctggtg tcgatgatgt agcacgaagt ttgttttatg ctgtgacagt
     4561 tatcgttata ttccttattg gatggggcgt tggcattgtt ttctttttgg gcataaaaca
     4621 aaagcgcaat atacaaaatt tgagttttat cagacttttt cttgcgatat tgctgctttt
     4681 tattccacct gttctggagt tttcggtaat tcatcagcat gttgagccag atgtactgat
     4741 tttttgcgtt attgctgtct ttataatcac gctttttgtc aggtcaggaa gaagacttgt
     4801 ttcagtcagt tgtttttcgg aaatgtattt tattcgccat caccgaattg agttcatgat
     4861 ggctgggttt atgatttatt tctgggcatt ctctcttatt gccggttggt acaaaccaca
     4921 gtttaagagg gaatatcagg cgatccacta tgagaatgtc tggtattaca ttattgcgcg
     4981 ctatgatgat cgtctggtgt tatcgaaatc atacagtagt gggggtaaga aattcgttat
     5041 atgtaatagc ggaaatattg atgattttga aattaatgtt gtcagggtgc gttaaaattt
     5101 cctgagtaac aaagattttt accgcccgcc attgagcggg ttttttatgt cagaaaagcg
     5161 gttcggtaca ttaaatgtgc aggtggttat taataccggt ctttcagctt gctggctttt
     5221 tcgacaagag ctattggtgt gtcacgttaa ccggaaaagg gaaaaagaca tgcagaaaca
     5281 gcaggatatg acagaaaccg ccagagtggt gtttaatgaa ttgagcgtca ccgaaccggc
     5341 gacagtcggg gagattgcgc agaacacata cctttcacgc gaacgctgcc agttaatact
     5401 gacccagctt gttatggcgg gtctggcaga ctatcagttt ggttgttaca gacaccttcc
     5461 acagtgaagg cttttttatt tgtggtaaat gggcggctgg tgggtgttag gggcgctcac
     5521 cagccatctg ctcatgcatt gggatcacaa gcaaacctca ggcccactgc tttgcgcaaa
     5581 agcagaatga gcctatcaga gacaggctta atgatccatg tttaatactg taaaaatatc
     5641 cagttgtgag ttaatcaacg ccgactgcct ggaatttatc cggtcgttac ccgaaaattc
     5701 tgttgatctg atagtcacgg acccgccgta ctttaaagtg aaacctgaga gctgggataa
     5761 ccagtgggcg ggtgatgaag attacctgaa gtggctggac cagtgtctgg cgcagttctg
     5821 gcgggtgctg aaacctaccg gaagtcttta cctgttctgt ggccatcgcc tggcgtctga
     5881 catcgaaatc atgatgcgtg aacgctttaa tgtgctgaac catatcatct gggcgaagcc
     5941 atccggacgc tggaacgggt gcaacaagga aagcctgcgt gcgtatttcc cggccacaga
     6001 gcgcattctg tttgcggaac attatcaggg accgtatcgc ccaaaagatg atggctatga
     6061 ggcgaagggc agggcactga aacagcatgt gatggccccg ctgatatctt actttcgtga
     6121 tgcgcgtgcc gctctgggga taacggcaaa acagattgct gatgccacag gaaagaaaaa
     6181 catggtgtcg cactggttca gtaccagtca gtggcagtta ccgaatgaac gcgattatct
     6241 gaaattacag gcgttgtttg cccgggtggc agaagaaaag catcaacgcg gtgaactgga
     6301 aaaaccccac caccagctgg ttgccgcgta tacctcactg aaccggcagt atgcggaact
     6361 gcagtgtgaa tataagcatc tgcggcggta ttttggcgtg acggcgcagg tgccatatac
     6421 cgatgtgtgg acgcacaagc cggtccagtt ttacccgggt aaacacccct gtgaaaaacc
     6481 ggcggagatg ctgcggcaga taatttgtgc gagcagccgt cctggtgaca tggtggcgga
     6541 tttctttatg gggtcgggct caatcataaa agccgctatg gcgcttgggc gtcatgcaat
     6601 tggtgttgaa ctggaagctg aacgttttga gcaaacggcc agggaagttc tgaatttaat
     6661 cagtaatggt cactgatatt gcagaattaa atccccgtga ttattattct gcattccggc
     6721 cctttagctc agtggtgaga gcgagcggct cataaccgcc aggtcgctgg ttcaaatcca
     6781 gcaagggcca ccatgccgtc actagctcat caggaaagag catcaacctt ttatgttgag
     6841 agtgcggggt tcgagtcccc ggtgacggtc cagtaaaaaa atgatgagct ggctttcagg
     6901 accgttttta attcacagca aaaagagccc gtggcgtgtt tatcgttttt cccgaactgt
     6961 aacagaaaac attgatagtt cctgtgccgt gttttataac gctgaatggg gtggggcatt
     7021 gttggtggga gttctggtac ttcctgacag ggactgacga tgcgccaccc cgacgttgtt
     7081 aaaattacga aaaaatcctg ttgtctgttt gaatgccgcc ttaactggcg gttttttgca
     7141 actttatacc ggactgtata tcaggtaagc attcagttat cgtgccatat aacaaaatcc
     7201 gaaaatgcta tgcctgcata ttgttagttg gcttatgttt ttgtatgttg ctgcaccgca
     7261 ggattctccc ctgcaacgaa ggttgtatga tgaataattt tttatctttc ctttttccag
     7321 tcatcctcag atgactggct cctttttgag cttgtcgata atacggggga tgccacttca
     7381 ggggataaag ttattgcgtt gccggggaag cattgtcagc tttttttaag ataactcctg
     7441 tgagttatgt gcataaatgc tacaaactcc acgttttata tggttgggag ttgccgggcg
     7501 cgcagttagt ttgctaagaa aacgcctgca tggtgaatcc ccctgtgcgg tggggtgata
     7561 ccattaattt cctgtgttgc cgactggtat cacggacggt ctgttc
//
LOCUS       DADTFG010000191         7601 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.192, whole genome shotgun sequence.
ACCESSION   DADTFG010000191 DADTFG010000000
VERSION     DADTFG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.192"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..205
                     /locus_tag="J1138_004223"
     CDS             <1..205
                     /locus_tag="J1138_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA8455941.1"
                     /translation="KKICRAEGATEEDDNKLVREFERLTEHQDGSDLIYYPRDDREDS
                     PEGIVKEIKEWRAANGKPGFKQG"
     gene            267..410
                     /locus_tag="J1138_004224"
     CDS             267..410
                     /locus_tag="J1138_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E7 lysis protein"
                     /protein_id="HBA8455942.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSTAELTGV
                     ETQ"
     gene            907..1899
                     /locus_tag="J1138_004225"
     CDS             907..1899
                     /locus_tag="J1138_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HBA8455943.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     ASARRSIDKNYGMGRNCHLFETLRKWAYRAIRQGWPDYERWLEAVIQRVEMLNSQLPV
                     PLSAAECRAIGKSVAKYTHRKFSPEGFSSVQAARGRKGGKANSSANQSDKGKKSAAVR
                     WTANDDKRRRALDMYILGASTEDIAVAVGVSSRTVRRWMDSSGEWLAAKQIIKY"
     gene            2204..2512
                     /locus_tag="J1138_004226"
     CDS             2204..2512
                     /locus_tag="J1138_004226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455944.1"
                     /translation="MRYLPQSVVAADICDSWARAYSSLPMCDRRLVAFWQLMARKFRD
                     GGRIRSSEYRELKGHLATAVVMADSPASFASPELRKAILNGALEAQKIILIIDPDLRG
                     "
     gene            complement(2549..3061)
                     /locus_tag="J1138_004227"
     CDS             complement(2549..3061)
                     /locus_tag="J1138_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455945.1"
                     /translation="MRFLLILRIITEDTPCTHRPQAIYCTTTTRRKKTPRSTTNMRIS
                     NIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEARLTEQERKLLHVLATAEKNDLQAQE
                     SERKQAIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTGELRYKKDKLLHKLKTL
                     KYDLETSHDT"
     gene            3118..4044
                     /locus_tag="J1138_004228"
     CDS             3118..4044
                     /locus_tag="J1138_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBA8455946.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIGRKGEYAKRKDLEYTEHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDARRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQDRTEPERVKSPSRGFSMGF"
     gene            4047..4580
                     /locus_tag="J1138_004229"
     CDS             4047..4580
                     /locus_tag="J1138_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455947.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTIIDRNYNATAAVYQKVVT
                     LEDKLAEKPVTGKSRKN"
     gene            4801..6546
                     /locus_tag="J1138_004230"
     CDS             4801..6546
                     /locus_tag="J1138_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HBA8455948.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVTAPMAFGFPALATPGAGTLGIAV
                     SGEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVT
                     DAQVSSLPLDQATVSVTKRVTDVVKDERQHIAVVSGVPMSVPVVDAKPTNTPGVFSAS
                     FPGVPSLTVSTVNDAPVSTSLPAGVTEDKDRTAFPAGFTFGGGSHEAIIRFPAESGQK
                     PVYVSVTDVLTPEQVKQRQEEEKLRQQEWDATHPVEVAEREYESAKAELEAEDKNVQS
                     LQATLDGLKNTPEGLALSDAGQHPLTSSESRFVAVPGYSGGGVHFDATATVDSRDRLN
                     SLINLGATAYVNNVLELGDVSAPTEDGVKVGNAIKNATIEVYDKLRQRLLDRQNEINN
                     AQASLNTAVESRNKKDENKRKAENKLNEEKNKPRKGSKDYGHDYHPAPKTEEIKGLGD
                     IRKGIPKTPKQNGGGKRKRWIGDKGRKIYEWDSQHGELEGYRASDGQHLGSFDPKTGD
                     QLKGPDPKRNIKKYL"
     gene            6556..6813
                     /locus_tag="J1138_004231"
     CDS             6556..6813
                     /locus_tag="J1138_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="HBA8455949.1"
                     /translation="MGLKLDLTWFDKKSEDFKGEEYSRDFGDDGSIIERLGMPLKDNI
                     NNGCFDVTNEWVPLLQPYFKHQINLSDSSYFVSFDYRDGDW"
     gene            7108..7359
                     /locus_tag="J1138_004232"
     CDS             7108..7359
                     /locus_tag="J1138_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HBA8455950.1"
                     /translation="MKLSPKAAIEVCNEASEKGLWILGIDGGHWLNLGFRIDSSTSWT
                     YSLPEEYKSKIPENNRLAIENIKDDIVNGYTAFIITLKM"
BASE COUNT         2035 a         1565 c         2042 g         1959 t
ORIGIN      
        1 aaaaaaaata tgtagagctg aaggtgctac tgaagaggat gacaataaat tagtgagaga
       61 gtttgagcga ttaactgagc accaagatgg ttcagatcta atttattatc ctcgcgatga
      121 cagggaagat agtcctgaag ggattgttaa agaaattaaa gaatggcgag ctgctaacgg
      181 taagccagga tttaaacagg gctgaaatat gaatgccggt tgtttaagga tgaatggctg
      241 gcattctttc acaacaagga gtcgttatga aaaagataac agggattatt ttattgcttc
      301 ttgccgccat tattctggct gcttgtcagg caaactatat ccgtgatgtt cagggcggga
      361 ctgtatcacc gtcgtcaact gctgaactga ccggagtgga aacgcagtaa cccgaaatcc
      421 tcatttgaca agaacaaagc gtgtcaggct gatcctgacg cgcttttttt tgaagtttca
      481 caaaaattcc atgcgggaga tgagatctaa aatccctgtg cagaactttc catccagggg
      541 gagaaaacgt gtcattttga cccgtttggt gttcagaaca cacgaaaccg atcgcgcatc
      601 gctttcgtgc atagttatgc aacgcgctaa aaacgattct gacgcatttt tcttgtttgg
      661 cctggtgttt ccttgtcttt ttgtgttttt tgcgtcagga agcgtctgag agcgttttaa
      721 ggggtacgta caacgggagt tatggtaaat ggatcggttt ttcgggaagg atcgacagga
      781 tttgccgttg ggtgtagtgt aagcgactga aaaacaaacg cccctgaaat tcgtgtctat
      841 ccggcaagac tgccacgaaa atgcagaggg cgtgtaggtc gcataacccg gagggaatcg
      901 taacacatga gcgccgcgct tcaatacttc gaagaaaatt tacctcatcg tccctatcac
      961 acggatgatc tcgctttcgg ccttcgcatc tctggcaaag ggcgtgcgct tcttgcgcgg
     1021 tacatccagc agaaccagcc acatgcgcag ttctggctgg tttttgacgt tgaccgcgag
     1081 ggagctgcta ttgactggag cgacctgaac gctcccgcac cgaacatcac cgtaaaaaat
     1141 cctgtgaacg gtcatgccca cctgctctac gcactcaata tcgccgtgag aactgcgcct
     1201 gatgcgtcgg ttaaggcgct gaaatacgcc gccgcgattg aacgtgcgtt gtgtgaaaaa
     1261 ctgggcgcag atgtgaacta cagcggactg atttgcaaaa atccgttcca cctggaatgg
     1321 caggtgatgg agtggcgcga ggaagcctat accctcgatg aactggctga ttatctcgat
     1381 ttgagcgcct cagcgcgtcg tagcatcgat aaaaattacg ggatggggcg aaactgccat
     1441 ctcttcgaaa cgctgcggaa atgggcttac agggctatcc ggcaggggtg gcctgattat
     1501 gaacgctggc tggaagctgt gattcagcgt gtcgaaatgc tcaactcaca gcttccagtt
     1561 ccgctttcag cggctgaatg tcgggctatt ggcaagagcg ttgcgaaata cacacacagg
     1621 aaattctcgc cagaaggctt ttcctccgta caggccgcac gcggtcgcaa gggcggtaag
     1681 gccaattcat cagcaaatca gtcggacaaa gggaaaaaaa gcgccgctgt tcggtggact
     1741 gctaacgatg acaaacgtcg tagggcgctt gatatgtaca tcttgggtgc gtcaaccgaa
     1801 gatattgctg tggcggttgg tgttagcagt cggactgttc gacgttggat ggatagttct
     1861 ggagaatggc tagctgcaaa acaaataatt aaatattaat caacaaaaaa ataacaaccc
     1921 ccccgtgttt ttgttatttt tttgttaaat ttgcggacat gaacaagcct tatcagataa
     1981 cagcgccttt gactgttttt tgagcagtcg ctttcaggcg gttttctccg cttctgtccc
     2041 gcaacggtgc tgccttccac atgattgaga ttgtagcgct cgccacagtc tcatgaccga
     2101 gcgtagctag cgaatgagcg aggaagcgca acggctttag gtggggcata tggcatttac
     2161 gcaccgtggt ttcccctttc ttctccttcc atcaggaggt tatatgcgtt atttacccca
     2221 gagtgttgtc gctgcggata tttgtgattc ttgggctagg gcatattctt ctctgcctat
     2281 gtgtgatcgg cgtcttgttg ctttctggca gttgatggcc cgaaagtttc gtgatggcgg
     2341 acgtatacgt tctagcgaat acagggagtt gaaaggtcat cttgcaactg ctgtggtcat
     2401 ggctgattcc cctgcttcat ttgcctctcc tgaacttcgt aaagccattc tgaatggggc
     2461 actggaagct cagaagatta ttttgattat cgaccctgac ttaagggggt aaggagccgt
     2521 tcacgaagcg gaaaaatcat tcttgggttt acgtgtcatg gcttgtttcc agatcgtatt
     2581 taagcgtttt aagtttatgt aataatttat ctttcttata acgtagctcg cctgtttttg
     2641 tgtcaaccag tcctgcttcg atcagtagcc ccgccgcgag aaagagacgg tgattgcgtt
     2701 cccgtcgttg tttctttccc tcgattcttt tttggatcgc ctgttttcgt tcgctttcct
     2761 gtgcctgtaa gtcgtttttt tccgccgttg ccagtacgtg gagaagtttg cgctcctgtt
     2821 ctgtgagtct ggcctcgtta tcgagcaggt caatgatctg cctctgtcgt gcggtttgtt
     2881 ctccgagctt cctgatgaat ccgatccgtt tcttcagcca ttcgatgttg ctgatgcgca
     2941 tgtttgtggt gctccttggt gtttttttgc ggcgtgttgt ggtcgtgcag tatatcgctt
     3001 gcggtcggtg ggtgcagggg gtatcttccg tgataattcg taaaattaga aggaagcgca
     3061 cttatatgtt tctctccttc gtcgagaaac agtgcgctcc tgcggagggc tgaaaccgtg
     3121 gcaacctatc acctcagcgt taaatttggt ggcaagggac aggctgcaaa ccacgccgat
     3181 tacatcgggc gcaaagggga gtatgcgaaa cggaaagatc tggaatacac ggagcacgga
     3241 aacatgccgg aatgggccag ggataatcct tcccatttct ggcaggcggc tgaccagttt
     3301 gaacgtgcga acggttcgac ttatcgggaa ctggaaatcg cgctgccccg tgaactgaca
     3361 ccggaacagc gccttgaact ggtgcaggat tttgtacgtc aggaagccgg cgaacggcac
     3421 gcctggagtt ttgccattca caatccgaaa gcgagcattg acggcggcga acagcctcat
     3481 gcgcatatca tgatgagcca acgtgtgaat gatggcattg accgtacacc ggaacagtat
     3541 ttccggcgct ataacgccag gtatcccgag cggggaggtg cgaaaaagga ttccggaagc
     3601 ctgactctca ctcagcaaaa agagcagttg cgggaattgc gaaaacgctg ggaggtgaag
     3661 cacaacgaac atatgagaaa gcatggcttt gaaaggggct tcattgactg ccggacgctg
     3721 aaagaacagg gcattgaacg acgtcccgaa gtgcatctgg gttttgaagc tgccggacgg
     3781 cttgatgatg cccggcgtca tgacatcatg caaaaacggt ctgaaccgga ctattcccgc
     3841 catcttgaac aggcgcgaac ggtcgggcat gacatcaggg aatggctgga cactccggtt
     3901 aagcaacatg aactggtcag aaggcttcaa catgatgttg cgcgggagca tggctttgac
     3961 atgagagaca gagtgagcag acaggaccga actgaacctg aacgggttaa atcaccgtca
     4021 cgtggatttt ctatgggatt ctgaacatgg ccagcacaga agaaaaatta ctcacgctcc
     4081 tgttatcggt ggagcatctg caacataccg caatggagca acagaaagct gttgctgatt
     4141 ctctggctgt ggcaagggaa acacttcgcg gatatcggga tatttctgac ggtgtggcaa
     4201 aacaggtcag ggatgaagtc agaaatgagg tgcagaagct ggacgtggca gggctgatag
     4261 gtgagcgcat cagccaggat tttcagcggc tggaagagtc agtcgcaaac atgaaggcaa
     4321 acgcggatgc cctgaacagg gatattgcga caattcacag caatatcgtg caggaataca
     4381 acacccttaa agggtatagc tggaaatggc tgggtggggt gtcagtggct tttctggtta
     4441 tgttcagtgt gctcggatgg ggaatgaaaa caataatcga caggaactac aacgccacag
     4501 cggcggtgta tcagaaagtt gtgacactgg aagataaact ggcggagaaa cctgtcacgg
     4561 gtaagagccg gaaaaactga cgcgcttcgt ttgtccaacg cctcacggac cgcgaaagga
     4621 tttcgcgaac cgttcaccgt ttccatgagt gactcctccg aactgccaaa aaatatccct
     4681 ccctgacttg acagaaaaaa taatggcgag tactttttga tctgtacata aaaccagtgg
     4741 ttttatgtac agtattaact atgtaatcaa ttgttttaac gcttaaaaga gggaattttt
     4801 atgagcggtg gagatggacg cgaccacaac agtggcgcac ataacacagg tggtaacatt
     4861 aatggcggcc ctacggggct tggtggaaat ggtggggctt ctgacggttc aggatggagt
     4921 tcggaaaata acccatgggg tggcggttcc ggtagtggcg ttcactgggg aggtggctcc
     4981 ggcaatggta atggcggggg gaatggtaat tccggtggtg gcagcaattc atccgtaaca
     5041 gcacctatgg catttggttt tcctgctttg gcaactcctg gtgccggaac actgggtatc
     5101 gccgtctctg gtgaggcttt atctgcggca atagcggata tctttgcagc cctgaaaggc
     5161 ccgtttaaat tcagtgcatg gggtattgcg ctttacggca ttctgccatc tgaaatagca
     5221 aaagatgacc cgaatatgat gtcaaagatt gtgacgtcat taccggcaga aacggtgaca
     5281 gatgcgcagg tcagttctct gccactggac caggcaactg tcagcgtgac gaaacgtgtg
     5341 acggatgttg tgaaggacga acgacagcat attgccgttg tttcaggtgt accgatgagt
     5401 gtacctgttg tggatgcaaa accaacaaac actccgggtg tatttagcgc atcatttcct
     5461 ggtgttcctt ccctgactgt cagcactgtc aacgatgcgc ctgtatcaac ctcgcttccc
     5521 gccggagtta cggaggataa agaccggaca gcttttccgg caggctttac cttcggtggt
     5581 ggctctcatg aggcgattat ccgcttcccg gctgagagtg gtcagaaacc ggtgtatgtg
     5641 tcggtaacgg acgttctgac acctgagcag gtaaaacagc gtcaggaaga ggagaaactc
     5701 cgtcagcagg agtgggacgc cactcatccg gttgaagtgg ctgaacgcga gtatgaaagt
     5761 gcgaaagctg aactggaggc agaagacaag aacgttcaga gtcttcaggc aacgcttgat
     5821 ggactgaaaa atactccgga aggtcttgct ctgtctgatg caggacaaca cccgttaaca
     5881 tccagtgaat cccggtttgt ggctgtcccc ggatacagtg gcggtggtgt acattttgat
     5941 gccacagcaa cggttgactc gcgtgacagg ctgaacagtc tgataaatct gggggcaacc
     6001 gcttatgtta ataacgttct tgaacttgga gacgtcagcg ccccaacaga ggatggggta
     6061 aaagtcggta atgcaataaa aaatgcaact attgaggtat atgacaaatt acgtcagcgc
     6121 ctccttgacc gacagaatga aataaataac gcccaggctt ctcttaatac ggctgttgag
     6181 agtcgtaaca agaaagatga gaataaaagg aaagcggaaa ataaattaaa cgaggaaaaa
     6241 aacaagcctc ggaaaggctc taaggattat ggtcatgatt atcatcctgc tccgaaaaca
     6301 gaagaaataa aggggcttgg tgatattaga aagggtatac caaagacgcc aaaacagaat
     6361 ggtggtggaa aacgcaaacg ctggattggt gataaggggc gtaaaattta tgagtgggac
     6421 tcccagcacg gtgaacttga ggggtatcgt gccagtgatg gtcaacatct tggctcattt
     6481 gaccctaaaa caggtgacca gttgaaagga ccagatccga aacgaaatat caaaaagtat
     6541 ctttgaggtg atattatggg acttaaacta gatttgactt ggtttgataa aaaaagtgaa
     6601 gacttcaagg gggaagagta ttcaagagac tttggagatg atggatctat cattgaacgt
     6661 cttggaatgc ctttaaaaga taatattaat aatggttgtt ttgatgtgac gaatgaatgg
     6721 gttcctctgt tgcagccata ctttaaacat caaattaatc tttcggatag ttcgtatttt
     6781 gtttcttttg attatcgtga tggcgattgg taatcaaagt tatcaaggat gagtcataaa
     6841 tgtctgattc aggtgttcag ggatgaatgc ttggatctat gacatacaat ggacagtctt
     6901 cattgcctat taatcgatct ttcagtcgtt cacttttaac ttaccgaacg ttttcgtacg
     6961 gttgaaccaa aaaacactaa gtaatatcct gattggtgga cgattaaata gttctatgga
     7021 gaaatgaaaa tggacaataa attatttgaa catacggtgt tatatgatag tggtgatgct
     7081 ttttttaaac taaaaggaaa tgcttctatg aagttatcac caaaagctgc aatagaagtt
     7141 tgtaatgagg cttcggaaaa aggtttgtgg attttgggta ttgatggtgg gcattggctg
     7201 aatcttggat tcaggataga tagttcaaca tcatggacat atagtctgcc ggaggaatac
     7261 aaatcaaaaa ttcctgaaaa taatagattg gctattgaga atattaaaga tgatattgtg
     7321 aatggataca ctgctttcat tattacgtta aagatgtaaa tagtgttgtg agataacttt
     7381 cgagaaaata ccttttgcaa ggaagaaaga ccaagtaggt ggtagggaac gctttgaatt
     7441 acatcataat aaaccaatca gtcaggatgg tagtgtctat gatatggata atatcagagt
     7501 gaccacatct aagcgacata ttgatattca tcgaggtaag taaaaatgga actgaaacat
     7561 agtattagtg attataccga ggctgaattt ctggagtttg t
//
LOCUS       DADTFG010000192         7553 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.193, whole genome shotgun sequence.
ACCESSION   DADTFG010000192 DADTFG010000000
VERSION     DADTFG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.193"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(294..419)
                     /locus_tag="J1138_004233"
                     /pseudo
     CDS             complement(294..419)
                     /locus_tag="J1138_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            461..691
                     /locus_tag="J1138_004234"
                     /pseudo
     CDS             461..691
                     /locus_tag="J1138_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            743..1678
                     /gene="ttcA"
                     /locus_tag="J1138_004235"
     CDS             743..1678
                     /gene="ttcA"
                     /locus_tag="J1138_004235"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBA8455951.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1807..3180)
                     /gene="dbpA"
                     /locus_tag="J1138_004236"
     CDS             complement(1807..3180)
                     /gene="dbpA"
                     /locus_tag="J1138_004236"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBA8455952.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDVSLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVICFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3658..4641)
                     /gene="zntB"
                     /locus_tag="J1138_004237"
     CDS             complement(3658..4641)
                     /gene="zntB"
                     /locus_tag="J1138_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBA8455953.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4896..6128
                     /gene="dgcM"
                     /locus_tag="J1138_004238"
     CDS             4896..6128
                     /gene="dgcM"
                     /locus_tag="J1138_004238"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBA8455954.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISSLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6149..6712)
                     /gene="smrA"
                     /locus_tag="J1138_004239"
     CDS             complement(6149..6712)
                     /gene="smrA"
                     /locus_tag="J1138_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBA8455955.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6907..7553)
                     /locus_tag="J1138_004240"
                     /pseudo
     CDS             complement(6907..7553)
                     /locus_tag="J1138_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
BASE COUNT         2039 a         1913 c         1724 g         1877 t
ORIGIN      
        1 agaaccattt ttacatatca agatatcatc accacccaca atgaattaag tatttttctt
       61 attctttaga ttagatacgt aatgaatccc taactccatt cgggtgtcat ggttctttca
      121 tttgaaatac attcatatcc aatgtaatat ttccatgcgc aaatataacc acaaaccata
      181 cccatcattt attacagaga attattttat tcaccgaaaa gtttcacaaa atatcccctg
      241 atagaaaact aaaaaacact gcataatatc gctattctgg cacttgcgaa aaattatatt
      301 ctgcacttgt tgtagcgttc tatttctccg cctggcaacg aatgcacaat gtccgccaca
      361 gtttctgttc taatttcttc ctgtgttacc gcatcctgta cgggtagcgc aacaccagca
      421 tgctaagcaa ggctttccca tcagatttca ctacgactat ttgggagcga gatggaatgc
      481 agcagtaaaa cgtgctggta ttcgccgccg taatccgtac catacgcgac atacttttgc
      541 ctgctggctg ttgacggcag gagcgaaccc ggcatttata gccagccaga tggggcatga
      601 aactgcgcag atggtgtatg aaatttacgg tatgtggatt gatgacatga acgacgaaca
      661 ggtagccatg ttgaatgcgc ggttatcgta gttgcaaagt ttgccctcaa tatgccccat
      721 ttagtaccag agaactgaaa taatgcaaga aaatcaacaa attacaaaga aagaacaata
      781 caacctgaac aaattacaaa aacgtctgcg tcgtaacgtg ggcgaagcca ttgctgactt
      841 caatatgatt gaagaaggcg atcgcatcat ggtttgcctc tccgggggta aagacagcta
      901 taccatgctg gagattctgc gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt
      961 ggctgttaac ctcgatcaaa agcaaccggg cttcccggaa cacgttctgc ccgagtatct
     1021 tgaaacgctg ggcgttgagt acaagattgt tgaagagaac acttacggta tcgtgaaaga
     1081 gaagattccg gagggcaaaa ccacttgctc actgtgttct cgtcttcgtc gcggtatcct
     1141 ttatcgcacc gcaacggaac tgggggcgac gaagatcgcg ctgggtcacc atcgtgacga
     1201 tatcctgcaa acgctgttct taaatatgtt ctacggtggc aagatgaaag gtatgcctcc
     1261 gaaactgatg agcgatgatg gcaaacatat cgtgattcgt ccgctggcct actgccgcga
     1321 gaaagatatt cagcgctttg ccgatgcaaa agcgttcccg attattccgt gcaacctgtg
     1381 cggttcacag cctaacctgc aacgtcaggt gattgctgac atgttgcgtg actgggataa
     1441 acgttatccg gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca
     1501 tctgtgcgat accaacctgt tcgatttcaa aggcattacc cacggttctg aagtggttaa
     1561 cgggggtgat ctggcgtttg atcgcgaaga gatcccacta caaccagcgg gctggcagcc
     1621 tgaagaagat gaaaatcagt tggatgagtt acggctgaat gtggttgaag tgaaataacc
     1681 aggatagcgc ccgatgcgca agcgtatcgg gctactctta tggaggccgg ataagacgcg
     1741 gccagcgtca catccggcaa tcccgaagaa gatgtttact cttgcaccct gcaattcaac
     1801 atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtag
     1861 ctgtttccat gctttatgag caacagcctg acgcaccgcg acataaacat gcgccggatg
     1921 cacggcgatt ttgccaatat ctgcgccatc aagcccgatg tctcctgtca gcgcacctaa
     1981 tacatcaccc gggcgcattt tggctttttt cccgccatcg atacacaacg ttgccatttc
     2041 tgcttccagc ggcacaatgg aactattcgc tggcggcgtt tgccagttaa gttttatctg
     2101 caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggggcacaga aactgatcgc
     2161 cagaccgcta tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc
     2221 ccacgccagc tcaaagttca ccaccagctc aagcgatttt atatccagac cacgcgcagc
     2281 aacatcagtc gcgaccagta cacgggcgct accgttagca aaacgtacca gggtctgatc
     2341 gcgatcgcgt tgctccagat cgccgtgtaa cgacaatgca ctttgcccta cttcattcag
     2401 cgcgtcgcag acagactggc aatctttttt ggtattgcaa aacaccacgc aagaggatgg
     2461 ctgatgcaag cttaataacc gttgcaacag aggaattttg cctttgctgg atgtctcata
     2521 aaattgttgt tcaataggtg gcagagcatc tgttgagtca atttcaatcg ctaaaggatc
     2581 gcgttgcact cttccgctga ttgcggcaat ggcttccggc caggttgccg aaaacagaag
     2641 tgtctgccga gatgctggcg caaaacagat gacatcatca atggcgtcgc taaatcccat
     2701 atccagcatg cggtcggcct catccatcac cagcgtattc aacgcatcca gtgatacagt
     2761 gcctttttgc aggtgatcca gcaaacggcc cggcgttgcc acgataatat gcggcgcatg
     2821 ttgcaacgaa tcacgctgca taccgaacgg ttgaccaccg cacaacgtca aaattttggt
     2881 atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg ccagttcacg
     2941 cgtaggacac agcactaaag cctgggtttg aaatagcgac acatcaattt gctgtaacaa
     3001 gccgaggcca aaagccgccg ttttgccgct gccggttttc gcctgcacgc gaacatcttt
     3061 tccggcaagg atcgccggaa gcgcggcggc ctgcaccggc gtcatggtta aataacccaa
     3121 ctcattaagg tttgtgagtt gggcgggagg caaaacattc agggtagaaa aagcggtcac
     3181 aatctattct cgtggtcatc gacgcaaagt cagcaggcgc gtatcctcgc agatctacgc
     3241 tcacgatgcg acaatttaat cggttcttca tcgggtggtg ggtcaggcat gggttgcggg
     3301 cgagggatcg gatcgggtac tggaacagga tcgtcaggaa tcggttcagg gacaggaatt
     3361 tgcaaataaa taagtgtcgt catatttccc tccggtcatt gggtggactc ttaaagggta
     3421 gacgctgata aataacaggc aaaaaaaagc cgactcatca aagtcggcgt cgtacgaatc
     3481 aattgtgcta tgcagtaatt caaaaaagga agtaagacaa tatggagcgc aacgcccatc
     3541 gcttgacgtt gcattcacct gcaagagaga tattgccctg aatgggtaga gagtttattg
     3601 acttcgctca aactttacgg cgtttttgta tacagacagc cggaaaaatt gcttttttta
     3661 caaccattta ctacgatgca accataaagc aacaccacca ataagaacaa ctaacagaat
     3721 acaaaaaatt gaaaatccaa attgccatcc gccgccaggg atcccaccaa ggttgacgcc
     3781 aaataaccct gtcagaaacg tactgggtaa aaagaccatt gccatcaacg acattgtata
     3841 ggtacgacga gctaaatttt cctgcatcac ctgagcgatt tcatccgcca tcacgccagt
     3901 ccgtgctata caggcgtcga tttcgtcaag accgcgccca aggcgatcgg caatatcctg
     3961 catccgacgg cgttggtcat cgctcatcca cggcaaacgt tcactggcaa gacgagcata
     4021 aacatcacgt tgcggtgcca tatagcgacg catcacaatt aattgtttgc gcagcagagc
     4081 cagaaatcca cgcggtggaa tttgctgatc gagaagatta tcttcgaggt cgataatttt
     4141 atcgtgcagc tgctcgataa attcgctgga atgatcggtc aacgcatcgc acacatccac
     4201 cagccacccc ccgcaatcgg tcggaccagt gccctcttca agatcgctca ccacatcgtc
     4261 cagcgccagc actttgcgtt gtcgggtcga aacaattaac cgcccgtcca tatatacacg
     4321 catagcgacc agttgatcgg ggcgttcatc ggtgctgccg tttatacagc gcaatgtaat
     4381 cagcgtgcct tcaccgagac ggctgactcg gggccgtgtg ctctcgcccg ccagcgcatc
     4441 acgtacgtta ttgggaagca gcggtgttgt cgccagccat tgggcgctat catggtgtac
     4501 ataattaagg tggagccagc agggatgcgc ttcatcaatc acatctgtat tttccagcgg
     4561 tttaacgccg cctctaccat ccagcatcca ggcaaatact gcatccggga cattaacgtc
     4621 cgatccctta atcgcttcca cagtgcctcc atcatcaacg cattattttg tagtctagcc
     4681 ttctggccct gttacgcaac atctcatcac cccattaccc tgaaatgatt aataaaattc
     4741 tgtctaaatt gaatacaaaa agcaaaatgc ttttccgtat acaaaccgtg tgaagtgtta
     4801 aatagcgtct atcattatca gaattatctg atcatatgac gtggcttttt tgcgatcgga
     4861 tagcaacaaa aattgataaa aataatggga tctcaatgat tacacacaac ttcaataccc
     4921 tggacttact caccagtcct gtctggatcg tttcgccctt tgaggaacag ttaatttatg
     4981 ccaacagcgc ggcgcgactg ttgatgcaag acctcacgtt tagtcagcta cgaaccggtc
     5041 cctattccgt ctcctcacaa aaagaactgc cgaaatacct ctccgatctg caaaatcaac
     5101 acgatattat cgaaatcctc accgttcagc gtaaagaaga ggaaacagca ttaagctgtc
     5161 ggcttgtttt gcgagagctg acagaaacag aaccggtgat tattttcgaa ggtatcgaag
     5221 caccggcaac gctgggttta aaagccagtc gctcggcaaa ttatcagcgc aaaaaacaag
     5281 gtttttatgc gcgctttttt ctgactaact ctgcaccaat gttgttgatt gacccctccc
     5341 gagatggcca aatcgtcgat gctaacctcg ccgcgctcaa tttctatggt tataaccatg
     5401 aaacgatgtg ccagaaacat acctgggaaa ttaatatgct cgggcgtcgc gtcatgccta
     5461 tcatgcatga aatctcgcat ttacccggtg gtcataaacc tttgaatttt attcataaac
     5521 tggcggatgg ttcgactcgt catgtgcaga cctatgccgg gccgattgaa atttatggcg
     5581 acaagctcat gttatgtatt gtgcatgata ttactgagca aaaacggctg gaggagcagc
     5641 tggaacatgc tgcccaccat gatgcgatga caggattact gaatcggcga cagttttatc
     5701 acattaccga gccaggccaa atgcagcacc tcgccatcgc tcaggattac agcttgttgc
     5761 tcatcgacac cgatcgtttt aaacacatta acgatctcta tgggcattct aaaggtgatg
     5821 aggtgttatg cgcccttgcc cgcactctcg aaagttgcgc tcgcaaaggc gatttggtgt
     5881 ttcgttgggg aggcgaagag tttgtcttat tgctaccaag aaccccactg gataccgcgc
     5941 tttcgctggc tgaaactatc cgcgtaagcg tggcaaaagt gagtatttcg agcttaccac
     6001 gctttactgt cagcattggt gtggcgcatc acgaaggaaa tgaaagcatc gatgaactgt
     6061 ttaaacgcgt tgatgatgct ttgtatcgag cgaaaaatga tggacgcaac cgcgtgctgg
     6121 cggcataagc cacggatgcg tctcgtgatc aacgactgcg cttagcgtgg cgctcccagt
     6181 tttcttgctt cgcctgcgct gttttacgta gtgcgacgta acacgccccg ctgccgccat
     6241 gatgcggtag agcagtgcaa tatgcctgaa catcatcaaa ttcggtcagc cagcgcgcca
     6301 catagctgcg gacaatattg gcatgcgatt tatcatcccg tcctttgcca tgaataatca
     6361 gcacgttacg caaaccatcc gccagggctt gttgaatgaa actgaacatc attttgcggc
     6421 actcttccac cggctggcgc aaaagattca ggctcgcctg ttgcggatat ttaccactgc
     6481 gcagcttatc cagcacccca tgttgcaacc cttcccgccg aaactccagc ggctgactta
     6541 gcgggatgat gtcgagaaat ccggtggtga ggaaattatc aagttgcagc gtgtcgatac
     6601 gctgcggcgc acgttggtta cgcgttggat gccagtggac atctgtagca cgtttcagcg
     6661 gctggacatc ttccatggcg tcaagaaaca gcgatttgtc gtcaaggttc atgtaacctc
     6721 ctatgcacct tcacacgtcg tgattcagac gggttaattg ttcatactta ctgcatcgtt
     6781 cccttcacag attgaaacaa tactctactt catacctgtg agtatctgcg caggcggggc
     6841 attttatact agcctgttag cagtatcgaa cagtttagcc cgagtttaat tcagcacagg
     6901 ctccgatcag gatgatttat tcatagcagg aaccaacaaa aatgcgataa ataacagacc
     6961 actgattagc agaaaggtat ccgaaaatgc catagtttgc gcctccctca gcatcaattg
     7021 actcaataat ttggttgaag ccagtatttc cgatgtctgg tcacttccac tgcgattaaa
     7081 aaacaaagca cttctggaaa tgaagtcatt caccgtatgg ggaacagaaa ccattttttc
     7141 acccattctc gaaaatgaaa attagttcga ttattgagaa tagagctaca cagggcaata
     7201 ccaattgcgc ccccaagatt acgtgttaaa ttaaatacac cagaggctaa cttcagccgt
     7261 tcttttggaa ttccccccag cgttaatgtt acgatggggg ccatcgcaaa ctgctgcgaa
     7321 atccctctaa ttgcctgtgg aaacagtaac tcctgccatc cccattcgtg ggttataggg
     7381 gtaaaaagat acattgaaaa cacaaatccg cccagccccg ccatcaacag ccatcgcaga
     7441 tttatttttt taattaacca gaagtaaaag ggaacagaaa ataactggaa aatacctgtt
     7501 gtgcaaacgg caaaacctat ctcttcggca tttaatccgc gaacctgccc caa
//
LOCUS       DADTFG010000193         7431 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.194, whole genome shotgun sequence.
ACCESSION   DADTFG010000193 DADTFG010000000
VERSION     DADTFG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.194"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            73..1200
                     /locus_tag="J1138_004241"
     CDS             73..1200
                     /locus_tag="J1138_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455956.1"
                     /translation="MLSVGVALTSLFASDELAKNGPYFWGLACGVPAFIWSLVGSVRW
                     LVFITQHIRADAWNQRREEVILQETRRGRRALQILSFSVQTALNGDSVAETTTAFLAR
                     QQVLNTYADLRGEDTVRRSVIPVLTNKPVTGRLSEIISRLFIDIRPQLFLLPPDFHIN
                     VLLEIDAPLSGASVRTIWQDEWQKAGLPEARLFSAPEPGLAAVDDWLDNFIQEKAVLL
                     VISVRLEPKNPERTAESATALLLANRLTQTALTPLALLHRPERITDTEMMASGIAQAL
                     DWMPVQPDAISGMWTAELDREQRAALLSLNQPFAQEALMYELDAFLGRSGPAAPWLSV
                     AAATLAAIQSQHPQLTLSGVQGGHYSWATVVSPFVSPQEAS"
     gene            1257..4586
                     /locus_tag="J1138_004242"
     CDS             1257..4586
                     /locus_tag="J1138_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HBA8455957.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELRRQLAAGDDYRAVTTKKADRRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLAALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELVNGSKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLPERCTPEQLAAMLEAQTLPLTEQGMSQLLADNRHDFLLRLAHTLAER
                     GIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPHAWLPSPVWAGITGDNAR
                     GRAVGFPWLRTALMSAVCVLVIWGAGMTTSFFANRALVQETGIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRY
                     ENIPEGVRQNVLPSLLTFWMANLPEHPQWETSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPD
                     PQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVL
                     TPAADSLNRQWQRAIVRLWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIADN
                     LSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKP
                     ARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRG
                     SWSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPMALLELKNFRLPG
                     QVFLTGKSMKDAEEYGEDADE"
     gene            4579..6189
                     /gene="tssA"
                     /locus_tag="J1138_004243"
     CDS             4579..6189
                     /gene="tssA"
                     /locus_tag="J1138_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBA8455958.1"
                     /translation="MSELLQKLTRSCFADRDALDVARTRAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVEHFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANM
                     IAALNLLTVSFAAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSSVTAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYVQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIATQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMDALLGTLVAINPVRAAVLCDTQHKE"
     gene            6189..>7431
                     /locus_tag="J1138_004244"
     CDS             6189..>7431
                     /locus_tag="J1138_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HBA8455959.1"
                     /translation="MTMGRGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRMTC
                     GKHPGMFIIVGGVPETDIHGRLMAGSLDSQSSCPCKARFIASMMDDVYEVETEKTENK
                     SVMPLNPLNNGTGNSSPPAYTSDYPALRNTHNLPDQQVRDMLANNNHDVMLLTYEEGA
                     EVLQSWGCQDFKRKWVDITQSAPGQLIINYGVNGKDVVTTSIVLARLGDFGIKATAYV
                     NHKGTELIKLTGYPGIRKILNAPVFAAKNPKIVDVGIGKYGLTNSIVKGARLTFYVAA
                     AYRTIDFILNDETSLAEFIGALATDVGKIAIASAVSWGAGKALLATGFVAGPLVGVVV
                     VGIGAALLLNYLDNKFGLTDQVVAYIEAAQQEFVEKAREIEEGIWDLGAMWAKHMLIK
                     GKEVLEHEVKEYLKDKLNNVSK"
BASE COUNT         1651 a         2034 c         2255 g         1491 t
ORIGIN      
        1 aacactattc ctgatgctga accctatccc gtaccgccag cccgcattaa atggctgaca
       61 gccttattac tgatgctgtc tgtcggtgtg gccctgactt cattatttgc cagcgacgaa
      121 ctggcaaaaa acggcccata tttctggggg ctggcctgtg gcgtgcctgc ctttatctgg
      181 tcactggtcg gttcggtgcg ctggctggta tttatcacgc agcatattcg tgccgatgcc
      241 tggaatcaac gtcgcgagga agtcatttta caggaaacac ggcggggacg acgggcgttg
      301 cagatactca gtttttccgt acaaaccgcg ctgaatggtg attccgttgc agaaactaca
      361 acagcatttc tggcgcgtca gcaggtgttg aatacttacg ctgatttgcg cggagaagat
      421 accgtgcgtc gcagtgtcat tcctgtatta accaataaac cggttaccgg gcgtttatcc
      481 gaaattattt cccgcctttt tattgatatc agaccgcaat tgtttctgct gccccctgat
      541 tttcatatca acgtactact ggaaattgat gcgccgcttt caggggcctc cgtgagaact
      601 atctggcagg acgaatggca aaaagccgga ctcccggaag cccgtctgtt ttctgccccg
      661 gagccggggc tggccgccgt ggacgactgg ctggacaact ttattcagga aaaggccgta
      721 ctgctggtga tttccgtgcg tctggaaccg aagaacccgg aacggacggc agaatccgcc
      781 accgcgctgt tgctggcaaa ccgactgacg caaaccgcgc tgacgccgct ggccctgctg
      841 catcgtcctg aacgtatcac agatacagag atgatggcgt caggcattgc acaggcgctg
      901 gactggatgc cggtgcagcc agatgccata tccggtatgt ggactgccga actggacagg
      961 gaacagcgtg ccgcgttgtt aagcctgaac cagccgtttg ctcaggaagc cctgatgtac
     1021 gagctggatg cctttctggg gcgctccgga ccggctgcgc cgtggctgtc cgtcgctgcg
     1081 gcaacgctgg cggcaataca atcacaacac ccccaactga cgctcagcgg cgttcagggc
     1141 gggcattatt catgggcgac cgtggtgtcg ccattcgttt ccccacagga ggcatcatag
     1201 tggttccggg attaaaccgt ctccggcccc gttcggtttc agggcctgcc atcgtcatga
     1261 ccgtggctgt tgtggttgcg ctttgcacgt ttattcaccg taaaccggca ttgctgggcg
     1321 ttgaaccggg tagctcacag caaatgtact ggatggctgg cgtgattgcc gctggcgtgg
     1381 tctttctgat tatcatgggg gtttatcatc tgctgaaccg ccacgctgcc gtacagagct
     1441 ttgaactccg cagacagctt gccgccgggg atgactatcg ggcggtaacg acaaagaaag
     1501 ccgacagacg tcatccggca atggtggaac tcgccgcctt cctgcgcgac cgctacggcc
     1561 cgttctggcg gcgtaaaatc cgcctgctgc tggtgaccgg cgagcctgaa caggcagagg
     1621 ccatcgcgcc ggggctgacc gggcaacact ggctggaggg cgaccacacg gtgctgatat
     1681 atggcggcag gccatcagcg gagcctgatg tcacactgct ggccgcctta aaaaaactgc
     1741 gccgcagccg tccgctggac ggcatcatct ggccactgac agaagaacag agccgtcaga
     1801 cagcgcaact cgacaaaggc tggcgcgagc tggtcaacgg cagtaagcga ctcggttttc
     1861 aggccccact ctatttgtgg caggtctgtg acaacggtga ttatcagacc ggacgccctc
     1921 tgcaaagcgt cggctgcctg ctgccggaac gctgtacccc ggaacaactg gctgcaatgc
     1981 tggaagcaca aaccctgcca ctgacggaac agggcatgtc gcagctgctg gccgataacc
     2041 gccatgattt tctgctgcgt ctggcccata cccttgcaga gcggggtatc gctcactggc
     2101 aaagcgttct gaaaccgctg ctggcaggcg gcgcattttc ctccctgcgt ctgcgtggcc
     2161 tgatgttcag cccgccgctt gccgccgtac cggaggccgc acctcatgcg tggctgccgt
     2221 cgccggtctg ggcaggcatc accggcgata acgcgcgggg ccgcgcggtg ggctttccgt
     2281 ggctgcgtac cgcactgatg tccgctgtct gcgtgctggt gatatggggg gccggaatga
     2341 cgacctcgtt cttcgccaac cgcgcgcttg ttcaggaaac cggtatccag acggcacgtg
     2401 cgcttgatac ccgcctgccg ctggcagaac aactggtggc gctgcatacc ctgcagggcg
     2461 aactggagcg cctgcaatat cgtatccgcg aaggtgcgcc gtggtatcag cgttttggtc
     2521 ttgaacgtaa ccaacaactg ctcgccgccg cctttcccgg ctatgcacag gcggcaaacc
     2581 ggctggtgcg cgacgtggcc gttgaccatc tgcaacagca acttaatgcc tttgtcgcgc
     2641 tgccgcccaa cagtcctcag cgtaccgcca ccggtgaaca acgctataag cagcttaagg
     2701 cattgctgat gacttcccgc ccggaaaagg ccgacgctgc ctttttcagt accacgctga
     2761 tggcagacgg cctgcgctac gagaatatcc cggaaggtgt gcggcagaac gtgttgccgt
     2821 cactgctgac cttctggatg gcgaacctgc cagaacaccc gcagtgggaa acctcaccgc
     2881 caccggaact gaccggcgcg gtgcgtaaaa tcctgctgcg ccagattggt gtgcgtaatg
     2941 ccgaaaacac cctctaccag aacgtgctgc aacaggtgtc ccgcaactac gccgatatga
     3001 cgctggcaga catgaccgga gataccctca ccgaatctct tttcagtacg gaacagacgg
     3061 tgccggggat gttcacccgt caggcgtggg aaggacaggt cagggaagcc atcgagcagg
     3121 tggtgacggc gcggcgcgag gaaatcgact gggtactcag cgaccgccag caggatacct
     3181 ctgcggatat ctcgccggat acgctgcgta accgtctcac ctctcgctac tttaccgact
     3241 tcgccggaag ctggctggcg tttctcaaca gcattcgctg gaaaaaggaa gactcactct
     3301 ccggcattct cgaccagctg acactgatgg ccgatgcccg tcagtcgccg ctgattgcgc
     3361 tgacggacac cctcgcgtgg caggcggcgg caggcaggga aaaccgtggt ctgtcagact
     3421 cgctggcgaa atcggcacag gaactgttta acggcaagga gaaaacgccg cagcaatccc
     3481 gtgaaggtga cgacgtgcct gtcgggccgc tggataaaac cttcgcgccg ctgctgcggt
     3541 tgctgggcga taaggccgga ggcggcgaca gccagctgag tctacagacc tacctcaccc
     3601 gcgtcacccg cgtgcgtctc aaactgcaac aggtgaccaa cgcccccgac ccgcaggaga
     3661 tgacccagca actggcgcag acggttttac agggcaaaac cgttgacctc accgacaccc
     3721 gcgactacgg acggttaatc gccgccagtc tgggcgagga atggagtggc ttcggtcagg
     3781 cgctgttcgt ccgcccggtg gaacagtcgt ggcggcaggt gctgacgcct gcggcggaca
     3841 gcctgaaccg ccagtggcag cgggcgattg tcagactctg ggatcaggac ttcgccggtc
     3901 gctacccgtt caaagcctca cagaacgatg catccctccc cctgctggcg cagtacctgc
     3961 gcgatgacgg gcgcatcaac cagtttattg ccgacaacct ttccggcgta ctgaaacgtg
     4021 aaggccgcta ctgggtggct gacgccatga acacgcaggg gctgacggtt aacccggact
     4081 ttatccgcgc cctgaaccgc ctgcgcgacg tggccgacac cgcctttgcc agcggcgatg
     4141 ccgggataca ttttgaactg cgggcaaaac cggcgcgtga cgtgatgaaa acacatctgg
     4201 tgattgacgg gcaggaactg gaatatttca accagaaaga acgctggcag cgttttaact
     4261 ggccggacga acagtggcaa cccggcgcat cgctgagctg gaccagcaca caggcgatgg
     4321 aacgcatact ggcggattac cggggaagct ggagtcttat tcgcctgctg gaacaggcgc
     4381 aggtgacgcc ggtggacagc agcaccttta aagtggtgtg gaaagcgcag gacggcctgc
     4441 cgctgaatta cctgctgcgg gttgaacagg gtaaagggcc gatggcgctg ctggaactga
     4501 aaaacttccg cctgcctgga caggtattcc tgaccgggaa gagcatgaag gatgcggaag
     4561 aatatgggga agacgccgat gagtgaactg ctgcaaaagc tgacccgtag ctgttttgct
     4621 gaccgggatg cactggatgt ggcgcgaaca cgggcggcgc tgtggcagac atggctgctg
     4681 ccggtgacgg cagacacgcc ggtcggggaa gaccccggct accacgacga tttcctgcgt
     4741 atacgcgacg aaatgaacaa actttccggc gcagataccg acctcatctg ccagcttgcc
     4801 gaatctctgc tgctgacgca ggccaaagac gtgcgcatcg ccacttacta catctgggcg
     4861 cgactgcacc gcgacggcga acgcggactg gctgaggggc tggcgctgct ggccggactg
     4921 gtggaacact tcggtacgca actgctgccc tcgcgcccgg caagccggaa aatggcgctg
     4981 gagtggctgg cgggggaaaa gatgctcgac agccttgccc gctacccgga agtggcaaaa
     5041 gaggattttg cgaacatgat tgccgccctg aatctgctga cggtctcctt cgccgcgtgg
     5101 cccgaagacc agcattcccc ctccctgatg ccgctgatta acgcgctgga atcacgtctg
     5161 gcgcagtcgg gtggaatgaa cgcggtggtg ccgcaaaaca gcagcagcgt tactgcacca
     5221 tcatccccgg tggatgcgcc gcaggtacag accatcacct ccgggcgtga cctgctcgac
     5281 caggcgaaag tgctggcccg ttatctgaat gaacagccac agggctggct ttcggcgcac
     5341 cggctgatga aaacccttcg atgggacacg gtgcatgaac tgccgccgga cgtggacggc
     5401 aaaacccggc tggctccgcc gcgcaccgaa tcacgcaacc agcttaaacg gctgtatgtt
     5461 cagcaaaact ggacggaact gctggagcag gccgacctga tgttcagcac cggcgtcagc
     5521 catttctggc tggacattca gtggtatctg catcaggcgc tgactaaggc gggcgcaccg
     5581 tgggacaggt ggacagccgt tatccgtcag gacctggcgc tgctgctgga gcgcctgccg
     5641 gggctggaaa atctggcatg gaacgacggc acgccgttcg ccgatgaggt gacgcgcaac
     5701 tggatagcgc agcaggtcat gatgcgggaa gacggcgcat ggcttgcggg gaaagccgct
     5761 gtaccgacag acgacgccac aaacgacgta ctggcgctgg agccggaagc actggagatg
     5821 gcagacagtc agggcgtgga agcggcgctg ggctggatac agacccgtcc gggcatcacc
     5881 acggcacgcc agcgcctgtt attgcggctg ctgatggcgc gggtggcgga acagtacggt
     5941 aaaaacgaga tggcgctcct gctgcttgaa gaactggata ttgccacaca gggaataacg
     6001 ctgacgcagt gggagccaga gctgctgttt gaggtaaaag cccgacaact gaaactgctg
     6061 cgtctgcggg cgcaccgcta tgcggataaa gccctgctga accgaaaaat ggatgccctg
     6121 ctgggtacac tggtggcaat caatccggtg cgggcggcag tactgtgtga tacgcagcat
     6181 aaggagtgat gacgatggga agaggttatt ttctggtcag gggcgataag accacctgcg
     6241 gcggcaaaat tattgaaggg gcggacgacc atacgatcat gggaatcccg caggcgcggg
     6301 atatggacag aatgacctgt ggcaagcatc cggggatgtt tattatcgtc ggcggcgtgc
     6361 cggaaacgga tattcacggc aggctgatgg cgggatcgct ggacagccag agcagttgcc
     6421 cgtgtaaggc gcggtttatt gcgtcgatga tggatgatgt gtatgaggtg gaaacagaaa
     6481 aaactgagaa caagtctgtt atgcccttga accctttaaa caatggaaca ggaaattcat
     6541 cacctcctgc ttatacctct gattatcctg cccttcgtaa tacgcacaat ctgccagacc
     6601 agcaggtcag ggatatgcta gcgaataata atcatgatgt tatgttactg acgtatgaag
     6661 agggcgctga agttctgcaa tcgtggggtt gtcaggattt taagcggaaa tgggttgaca
     6721 tcacgcaatc tgcaccaggg cagttaatta ttaattatgg cgtaaatggt aaagatgtag
     6781 tgacaacttc aatcgtgctt gcccgcctgg gggattttgg tatcaaagca actgcctatg
     6841 ttaatcataa aggaaccgaa ctgataaaac ttacaggata tcccggcatt cggaaaatac
     6901 tgaatgcacc ggtgtttgca gctaaaaacc ccaaaatagt agatgttgga attgggaaat
     6961 atggactcac aaactctata gtaaaagggg caagattaac gttttacgtc gcagctgctt
     7021 atagaactat tgatttcatc ctgaatgatg aaacatcgtt ggctgagttt ataggtgcgt
     7081 tggcaacaga tgttggtaaa attgcgatag cttcagcagt tagttggggc gcaggaaagg
     7141 cgttactggc cactgggttt gttgctggac ctttggttgg ggtagtggtt gtggggatcg
     7201 gcgcggccct tctattaaac tatcttgata ataaatttgg tttaactgat caagttgtcg
     7261 cttatattga agcggctcag caagaatttg ttgagaaggc aagagagatt gaagaaggga
     7321 tttgggatct gggagccatg tgggcaaaac acatgcttat taaagggaag gaagtactgg
     7381 aacatgaagt gaaggagtat ttaaaagata aactaaataa tgtgagtaaa t
//
LOCUS       DADTFG010000194         7267 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.195, whole genome shotgun sequence.
ACCESSION   DADTFG010000194 DADTFG010000000
VERSION     DADTFG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.195"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            24..398
                     /locus_tag="J1138_004245"
                     /pseudo
     CDS             24..398
                     /locus_tag="J1138_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552588.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            550..1596
                     /locus_tag="J1138_004246"
     CDS             550..1596
                     /locus_tag="J1138_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBA8455960.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1593..2384)
                     /gene="nei"
                     /locus_tag="J1138_004247"
     CDS             complement(1593..2384)
                     /gene="nei"
                     /locus_tag="J1138_004247"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBA8455961.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2420..3154)
                     /gene="pxpA"
                     /locus_tag="J1138_004248"
     CDS             complement(2420..3154)
                     /gene="pxpA"
                     /locus_tag="J1138_004248"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBA8455962.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQYGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            complement(3144..4076)
                     /gene="pxpC"
                     /locus_tag="J1138_004249"
     CDS             complement(3144..4076)
                     /gene="pxpC"
                     /locus_tag="J1138_004249"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBA8455963.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(4070..4726)
                     /gene="pxpB"
                     /locus_tag="J1138_004250"
     CDS             complement(4070..4726)
                     /gene="pxpB"
                     /locus_tag="J1138_004250"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBA8455964.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4749..5492)
                     /gene="ybgI"
                     /locus_tag="J1138_004251"
     CDS             complement(4749..5492)
                     /gene="ybgI"
                     /locus_tag="J1138_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBA8455965.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5763..7244
                     /gene="dtpD"
                     /locus_tag="J1138_004252"
     CDS             5763..7244
                     /gene="dtpD"
                     /locus_tag="J1138_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBA8455966.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         1603 a         2024 c         1819 g         1821 t
ORIGIN      
        1 aaaatatttc accggaaaca ctggtgattg atttgggtaa atttaactta ctggatattc
       61 gccgccacac aatgtcgaaa acattcagta tcaagacaac aaaatcgcaa aatgaccagt
      121 gtaccgacgg atttaaggtc agctcctcgt tttataccga aaaaacgttg gttgaagaag
      181 ataaagcatt gttgattggt aatggtctga agctacgtat actggatgaa aacgcctcgc
      241 cttatacatt caataaatat gcttaatacg ccgatttcac cagtgatatg ctgatctacg
      301 aaaaaaccta tacggctgaa ttatcgtcca cacctggcac tcccatcgcg gtgggtccct
      361 tcgatacagt ggtacttttt aagattaact ataactgatt taacacgacg cccttttcag
      421 ccggaaaggg caacgtattc agattattgc tgattttccc cttttccttg attaccgtta
      481 ttttttccag acccagcttt ctttatgctg tatcgataca ccaaagataa aataatgagg
      541 aagcgaagaa tgcctgtatt gcagtgggga atgttatgtg ttctgtcact tctcctttct
      601 attggcttcc tcgctttaca tctcccggcg gcactcctgc ttggcccgat gatcgcgggg
      661 atcatcttca gtatgcgcgg agtcactctg caacttcccc gctccgcttt tctcgctgcc
      721 caagccattc ttggctgcat gattgcacaa aacctcactg gttcgattct caccacgctg
      781 gccgtcaact ggccgatcgt gcttgcgatt ttactggtga cgctgctttc cagcgccatc
      841 gtgggttggt tattggtgcg ctatagctca ctgcctggaa ataccggggc ctggggttct
      901 tctcctggcg gcgcggcggc aatggtcgca atggcgcaag attacggcgc agatattcgc
      961 ctggtggcgt ttatgcaata tctgcgagtg ctgttcgtcg caggagccgc tgttctggtc
     1021 acccgcatga tgctgggcga taacgctgaa gcggttaacc agcagatcgt ctggttcccg
     1081 ccagtgagca ttaatctcct gcttacgatt ttgctcgcgg tcgttgccgg tacggcggga
     1141 tgcttgctac gtcttccttc cggcacgatg ctcatcccga tgctggcggg cgcagtgctc
     1201 cagtcaggtc agctcatcac gattgaactc ccggaatggc tgctggcgat ggcatatatg
     1261 gcaattggct ggcggattgg tcttggtttc gataaacaaa tattactgcg ggcattgcgc
     1321 ccgctaccgc aaatcctgct gtcgattttt gctctgctgg ctatttgtgc gggtatggcg
     1381 tgggggctga cccggtttat gcatattgat tttatgaccg cctacctcgc caccagcccc
     1441 ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca atgccgatat ggcgctcatc
     1501 atggcgatgc aaaccctgcg cctgttcagt attttgctga cggggcctgc catcgcacgg
     1561 tttatttcaa cctatgcgcc gaagcgttcg gcctagtgct ggcagccagg gcaccagtaa
     1621 aacgggcgcg atgacagcgt ggttttctca atgatgccgc cacagcgttc gcacggttcg
     1681 ccatcacgat gaaaaacctt aaagcgaaac agcgccccat gatatttgtt ttcatccacc
     1741 tgcccccgcg tagcgtaaga aagtcgcgga atatccagta acgcgtgtgc gagtgcatcc
     1801 agttgcgccg cattgagatc tttcgcttta tgatttccgg tcaacccaac ctgccagagg
     1861 atctccaccc gcaaataatt gccaagtcct gccagaaacg cctgatcgag cagtaatcca
     1921 gcaaactggc ggttacgaaa gcgcggcgac aataatcgtt cctttaccac ctccggcgtc
     1981 agattcggat ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt
     2041 tccggagtca acatctcaat atccgaggcg ctataaagca gaatggtttt gtcagccgtt
     2101 tgcagtttta cccgcagtac tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc
     2161 cagacgccgt aaagctgatt atggctgtag agcgttaagt cgttggaaaa atgagttaac
     2221 aacgccttac cacgcgtttc cacatgggta acgtgttgac cgataagtcg tgattgataa
     2281 gattttaact gcgggaaggc aaaccagaca tcagttagtg gtttgccttt gatcgccgcc
     2341 tccaggttat ccgctgcacg gcggatctcc gggccttcag gcatggtgat atcctttttt
     2401 cattctttat taagcggggt tatgctgcga caacaatccc cttttcagca aatgtagctc
     2461 gtaatcggcg ggcgaacgcc agtgcgtgct cgccatcgcc atgtaggcag accgtttgcg
     2521 ccgtgaccat tgcccattcg ccggtgatac ttttgactct gccgtattgc accatctcca
     2581 acgtttgcgc cagcgcctgt tcttcgtttt caatcaacgc gcctggctgg cttcgcggca
     2641 ccagcgaacc gtctgcctga taaccgcgat cggcaaacac ttcctcgcgc gttaccaggc
     2701 catatcgttc gcctgcacga atcagctcgc ttcctgccag cccgacgaga atcaatgccg
     2761 gatcgcaagc gtataccgct ctggcgatgg cgtctgccag ttgtgcctct ttcgccgcct
     2821 ggttgtataa catgccgtgc ggtttgacat gacacattac gccgccttgc gcacgggtaa
     2881 tcgctgccag cgcgccaatt tgatacagcg tctgggcgaa aacggtttct ggcggcagct
     2941 gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact cgggtgcgcg ccaatcgcga
     3001 caccgttttt tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg cctgcatgaa
     3061 atccacaggc aatattggca gaggaaacca gcgttaatag ttctgcgtcg ctggcgcagc
     3121 cttcgcccag atcggcgttc aggtcaattt tcattgtgca gccgccacgc taattgttcg
     3181 aaataacgtt gttgatcttg ccgtgctttc agtgcctctt ccagtgaaca ctggacaaaa
     3241 tgaatcggct gaccgagcgg aatttgcgcc agatggtaca tatcagcctc aatgatacag
     3301 gcaatacgcg ggtaaccacc ggtggtttgc gcatcgttca tcaacacaat gggctgcccg
     3361 ttatgcggca cctgcaccac acctggtaac aaaccgtgag ataacagttc gcgatcggtg
     3421 gtgcgtttta aaatttgccc ctgtaagcga tagcccatcc ggttactttg cgagctaagc
     3481 tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg atattccggc
     3541 cccggcaagg cgcgaatgcg gttgccccac agcagctgtt taacgccctg cgcttccata
     3601 aaatcacgct tcgctttgcc aatcgggagt cggtcaccat ccctcagtaa acggccttcc
     3661 aacccgccaa tccccacttt gagatcggtg ctgcatgatc ccattaccgg cggaacatca
     3721 ataccacccg cgaccgccag ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa
     3781 cgctggcctg ctctcattgg caatcgccag ccggtccaga cggcgttatc atccagccgc
     3841 gcttcgcaac cggcacccgt cagggcaaac cacccatcag tttcgaactc aaccgttaac
     3901 tgaccgagcg tgatctccag cgcgggggcg ttggcgtcat tacccaccag caggttagca
     3961 atgcgcagcg caggcatatc cagtgcgccg cagtggctga tacccgactg gcgaaaaccg
     4021 tgacgaccgc catcctgcac agtggtatac atgcccgcac gaataatctt cagcatactc
     4081 cctccttctg gggtacaaag cgcacgctgt ctcccggacg taataagatg ggttcgtcac
     4141 gctccggatc aaacaggctg agtgaggtat gaccaatcaa ctgccagcca cccggcgttg
     4201 ccagcggata aacaccggtc tgcggcccgc cgatcccgac agaacctgcc ggaacgtgca
     4261 agcgcggttc agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg agatacggga
     4321 agcccggttg aaaacctaaa aaccagacca cgtattccac ggaggagtgc aattcaacaa
     4381 cctgtttttc gctcaatcca caatgcgccg cgaccaccgc caaatccggt ccggctgcgc
     4441 caccgtaaac caccggaatt tcaataaagc gagactccgg ctccagcgcc tcactctcct
     4501 cccaccagcg ttgcaaacgc tctatggcat ccagcgccag cgactcagga ttacgcaaaa
     4561 tcactgtgat attgttcatg ccaggaatgg cttcaaccac attcggcata tccaccagac
     4621 gctgtgccag tcgccagatc cgtttctggc tagccagcgt caccggcggt tccagttcca
     4681 gcactaccgc cgtttcacct atcagataac aacgcgctcg ttgcactttc gatccctctg
     4741 attattcgtt atgcaggatt aggaatatca ataaaggtca catcaagatc ggtattttca
     4801 ttcagccact cgctcaatgc gcgaatacca ccacgttcag tggcatggtg acctgcagca
     4861 taaaaatgca atccctgctc gcgggctgaa tgaatggtct gttcagaaac ttcgccagta
     4921 ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa aactttgccc gccgcccgtg
     4981 caccaggcga cgcgctggac cacctcaggt ccggtatcgc cacaccataa cggcttacgt
     5041 cccagacgcg cttcaatcca ggaggccagt tccagtcccg gcacaggcat ggtcagttcg
     5101 ccccacggca ccaacggctc aatttcgccc atgaccgtga tccccagtaa cgccgccagc
     5161 tgtgcgttat tgcccagctc aggatgtgcg tcaagcggca aatgccagcc atacaggttg
     5221 atatcattcg ccaacaatgt ttttaagcgg ttacgcttca ttccgcgaat gaccggagat
     5281 tcgcctttcc agaagtagcc atgatgcaca atgaccgcat ccgcgcccag acgcactgcc
     5341 tcatcgagca aagcctggct ggcagtgaca ccggtaacaa ttttttgcac cgtctctttg
     5401 ccttccacct gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg
     5461 ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta
     5521 caccgttaac atagcgttac ctcgcctgtt cgtcgatagc caatcttttg acatattaag
     5581 aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca tcacgcttca gctaatgtat
     5641 atttatgcaa tcagacagca tattgcataa ataatcagat ctgtatcaaa gttttaactt
     5701 taaggaaaca caagcacgcc gcgcaactat tacgtgacgt tgcagtcaat tgtggaatta
     5761 ttatgaataa acacgcatca caaccgcgcg ctatttacta tgtcgttgcg ctgcaaatct
     5821 gggaatattt tagcttttac ggcatgcgtg ccctgctgat cctctatctc accaatcaac
     5881 taaaatatag cgataatcac gcctacgagt tatttagcgc ctactgttcg ctggtatatg
     5941 tcacgccaat cctcggtggc tttttggcgg ataaagttct cggcaatcgc atggcggtga
     6001 tgctgggggc gttgttgatg gcgatcggtc atgtggtgct gggtgccagt gagatccatc
     6061 cgtcattcct ctatctgtca ctggcgatta tcgtctgcgg ctatggtctg tttaaatcca
     6121 acgtcagttg cctgctcggc gagctgtatg agccaaccga tccacgtcgt gatggcggtt
     6181 tctcgctgat gtatgcggcg ggtaacgtgg ggtctattat cgcacctatc gcctgtggtt
     6241 tcgcccagga agagtacagt tgggcgatgg gctttggcct ggcggcggtg ggtatgatcg
     6301 cgggtctggt cattttctta tgtggcaatc gtcatttcac acatacccgc ggcgttaaca
     6361 aaaaggtact gcgtgcgaca aactttctcc tgcccaactg gggatggcta ctggttctgc
     6421 tggtggcaac gcctgcgctg attaccgtgt tgttctggaa agagtggtcg gtatacgcct
     6481 tgattgtcgc gacgatcatt ggccttgggg ttctggcaaa aatttatcgc aaagcagaaa
     6541 accaaaaaca gcgtaaggag ctggggctga ttgttaccct caccttcttc agcatgttgt
     6601 tctgggcatt cgcacaacag gggggtagct cgattagcct ttatatcgac cgcttcgtta
     6661 accgcgatat gtttggttat accgttccga ctgctatgtt ccagtcgatt aatgccttcg
     6721 cagttatgct gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc
     6781 gtaccgtgcg catctgggga aaatttgctc ttggccttgg cctgatgagc gccggattct
     6841 gcattctgac cttaagcgcc cgctggtccg caatgtatgg tcactcttct ctgccactga
     6901 tggtgttagg cctggcggtg atgggctttg cggaactgtt tatcgatccg gttgctatgt
     6961 cgcagattac gcgcattgaa atccccggcg tgaccggcgt attaaccggc atttatatgc
     7021 tgctttccgg cgcgattgcg aactatctgg cgggcgtgat tgccgatcag acatcgcagg
     7081 cttcgtttga tgcttccggg gcgatcaact actccatcaa tgcatatatt gaagtatttg
     7141 atcaaattac ctggggcgca ctggcgtgtg taggactggt actgatgatt tggctgtatc
     7201 aggcgcttaa gttcagaaac cgcgcgctgg cgctggagtc ttaataagcc agcaccatgc
     7261 ctgataa
//
LOCUS       DADTFG010000195         7198 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.196, whole genome shotgun sequence.
ACCESSION   DADTFG010000195 DADTFG010000000
VERSION     DADTFG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.196"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            14..247
                     /locus_tag="J1138_004253"
     CDS             14..247
                     /locus_tag="J1138_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8455967.1"
                     /translation="MREISDNMLDSVKGGMNINGLPGSTNVIDLRGKDMGTYIDANGA
                     CWAPDTPSIIMYPGEDGPSYSISSSTSSANSGS"
     gene            325..624
                     /locus_tag="J1138_004254"
     CDS             325..624
                     /locus_tag="J1138_004254"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA8455968.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELAILQKAATSFAKRLK"
     gene            621..1475
                     /locus_tag="J1138_004255"
     CDS             621..1475
                     /locus_tag="J1138_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA8455969.1"
                     /translation="MKYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQ
                     FRLTCDAAVHKAFFEAKQRYGAPRLADEMPEFNIKTIAASLRRQRLRAKASRKFSPVS
                     YRAHGLPVLENLLEQDFSASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSM
                     SLRMTAQLACDALQMALWRRRRPESVIVHTDRGGQYCSGDYQALLKRHNLRGSMSAKG
                     YCYDNACVESFFHSLKVECIHGERFSSREIMRATVFNYIECDYNRWRRHSACGGLSPE
                     QFENHNLA"
     gene            complement(2352..3386)
                     /locus_tag="J1138_004256"
     CDS             complement(2352..3386)
                     /locus_tag="J1138_004256"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBA8455970.1"
                     /translation="MQSVSKSIYRGKLLGSLPAVGEIHKEIAVSEETVTWISLQREII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVEYAKRLSVLQNKYLSQMYIVMRTYFEKPRTR
                     KGWKGIMHEPDLNGSYNVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAVGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAILAAREQHVVYMTSLTKC
                     ISTLLTDGNPHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVA
                     ERQISVAREVIDNRKKMPGYVAGIMLESFLQGGKQSDSLPREYGQSVTDECLSWQQTE
                     QLLSTLAAQL"
     gene            3667..3909
                     /locus_tag="J1138_004257"
     CDS             3667..3909
                     /locus_tag="J1138_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HBA8455971.1"
                     /translation="MAFARAQRRSHNRLGFAIQLALVRALGGTLRTEKLPPKAVITIV
                     AEQLGIDLAVLKLNVTNGAKVVLPQPEWKKMDETFW"
     gene            3935..5557
                     /locus_tag="J1138_004258"
                     /pseudo
     CDS             3935..5557
                     /locus_tag="J1138_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            5607..5750
                     /locus_tag="J1138_004259"
                     /pseudo
     CDS             5607..5750
                     /locus_tag="J1138_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5902..6115)
                     /locus_tag="J1138_004260"
                     /pseudo
     CDS             complement(5902..6115)
                     /locus_tag="J1138_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6258..6821)
                     /locus_tag="J1138_004261"
     CDS             complement(6258..6821)
                     /locus_tag="J1138_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA8455972.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHKKS
                     YRISRQLGDIWRRITEGEGYFHLCRTKDKYPVRIDHVIHYSDKSAVDGLRYALSYLAK
                     QDQKEHGIILMRSRLPEKSNRGRPRHN"
BASE COUNT         2002 a         1704 c         1622 g         1870 t
ORIGIN      
        1 aggagatttt aaaatgagag aaatatcaga taacatgctt gattctgtga aaggagggat
       61 gaatattaat ggattacccg gttctactaa tgtaatagat ctacgtggaa aagatatggg
      121 aacatatatt gatgccaatg gagcatgctg ggctccggat actccatcca tcatcatgta
      181 tccgggcgaa gatggacctt cttatagtat aagtagttcc acatccagtg caaacagcgg
      241 cagttaaata aataccctcc gtgatcttac ccaccaatag tggacacagg actaagtgag
      301 taaactctca accagaggtg actcatgaca aaaccagtat caatcagcaa gaagccccgt
      361 aaacaacata cgcctgaatt tcgtaacgaa gccctgaaac tcgctgaacg catcggtgtg
      421 gccgccgcag cccgtgaact cagcctgtat gaatctcagc tttatgcctg gcgcagtaaa
      481 cagcagcaac aaatgagttc gtcagagcgc gaaagcgaac tggccgctga aaatgtccgc
      541 cttaaacgac aactggcgga gcaggctgag gaactggcca tcctccaaaa ggccgcgaca
      601 tccttcgcga agcgcctgaa atgaagtatg tcttcatcga aaatcatcgg gcagagttca
      661 gcatcaaagc gatgtgtcgt gtacttcggg ttgcccgcag cggctggtat gtctggctca
      721 ggcgtcgtca ccagatgagc ctgcgccaac agtttcggct cacctgcgat gccgctgtcc
      781 ataaggcatt ctttgaggca aagcagcgat acggtgctcc ccgccttgct gacgaaatgc
      841 cggagttcaa tattaaaacc attgccgcca gcctgcgtcg tcagaggctg cgggcgaaag
      901 ccagccggaa gttcagcccg gtcagctacc gtgcacatgg cctgcccgta ttggagaatc
      961 tgctggagca ggacttcagc gccagcggcc cgaaccagaa gtgggcgggt gacatcacgt
     1021 acttgcgtac cgatgagggc tggttgtatc tcgcagtagt catcgacctg tggtcacgcg
     1081 ccgtcattgg ctggtcgatg tcactgcgaa tgacagcaca actggcctgt gatgcactgc
     1141 aaatggcgtt gtggcggaga agacgcccgg aaagcgtcat tgttcatacg gaccgtggtg
     1201 gtcaatactg ttcaggggat tatcaggcgc tgctgaagcg acacaacctg cgtggcagta
     1261 tgagtgcgaa aggctactgt tatgacaatg cctgtgtgga aagcttcttt cattcgctga
     1321 aggtggaatg catccacggg gaacgcttta gcagccggga aataatgcgg gcaacggtgt
     1381 ttaattatat cgagtgtgat tacaatcgct ggcgtcgtca cagtgcctgt ggcggtctca
     1441 gcccggaaca atttgaaaac cataatctcg cttagggccg tgtccacatt acgtgggtag
     1501 gatcatatta cctgtatgaa actcacatcc cacggtacgg atcacggtca cgcctatcaa
     1561 ctcatgctgg gcagcgatgt taaattgcac accattccga tgagataccc tgactgacct
     1621 tgaacaaaag gtttacgcta ctttgtatta tgttgttcac tggtgaatac tcccttagca
     1681 aggtacccat aactcaggga ggtaaaagta caatcagggc tggcagcact gcatgtgctc
     1741 tccgggggtt taccagaaca ttttctcatt taacgcaaca ttgtgaacag cgaagacgtg
     1801 tctggcaaga acgataccaa tgatagtcat aatgaattcc tcaagaacta ttgatgactt
     1861 tagcatcatc tccacagaca tattataaaa tatgatttag catgcatccg attattgatt
     1921 agcgccctac cgtgttcttt ggaaattaac tttttatcat agtcttaatt actcttttta
     1981 agccaacaga gacaaaatag caccgcaacc atgtcacaga tgacacctac gcaagtacag
     2041 tatcatttgc caagaagggc tagggaatga agcataagag ctaactggtt tgacaatata
     2101 catacacata ggccccacag ataataccct ggaagataca tccattggag atggtatatt
     2161 agaaatactg caactcctaa taaaaatatt aatgtcagag tgataacact tctgccattc
     2221 atgtcatatt ttatttccct tccaacaatg aactgtacag ataaaacatc tgtttctttg
     2281 atgcccagat tgggtgtatt aagataaata atttcatttt tcccttaaaa aatgatactg
     2341 aatagttgaa attaaagttg tgctgccaga gttgataata attgttctgt ctgttgccat
     2401 gaaagacact catcagtaac agactgacca tactctcgcg gaaggctgtc tgattgtttt
     2461 ccaccttgta aaaagctttc cagcataatc ccagctacat atcccggcat ctttttccga
     2521 ttatcaatga cttcacgagc aacagagatt tgccgttcag ccactttacc actattacca
     2581 tggctacagt caattatcag acgatgattg ataccttcat catgcattaa tttcactgcc
     2641 ttcgttatat ccgacaaacc atagttcggc tctctaccac cacgtaatat gagatgtcca
     2701 tgtggattgc catcggttaa tagggtagat atacattttg ttaaagatgt catatataca
     2761 acatgttgtt cacgggctgc aagaatagca tcaatagcta agttaatatt tccatcagtg
     2821 ctgtttttaa atccaacagg acaatgtaaa ccagacgcaa gttgacgatg cgtttgtgat
     2881 tcagtagttc tggcaccaac agccccccaa catattaaat cagcaatata gggagtaagg
     2941 aaaggatcga ggaattctgt agcggttgcg acacgcattg ttgtgattga ggacaaacac
     3001 tggcgagcat atcgtatccc tttctcaaca ttatagcttc catttagatc aggctcgtgc
     3061 attattcctt tccagccttt acgagttcga ggtttctcaa aataagtgcg cattacaatg
     3121 tacatttgcg ataagtactt attctgtaat acggacaatc gtttcgcata ttcaacggca
     3181 gcctgaacgt catggattga gcatggacca acaatcacaa gcaagcgagg atcttttccc
     3241 agcaaaatat tcgctataat ctcccgttgc agagagatcc atgttacggt ttcttctgat
     3301 actgcgattt ctttatgaat ctcgcccaca gcaggtaaac tgccaagcaa cttccctcgg
     3361 tagattgatt tactgactga ctgcattgta tctcctgaaa gttctattct taaccatctg
     3421 ttgcacatac taattgataa tgataatcat ttaatacatg tttgacaata caggatgttc
     3481 cgtaaccatt tctgcgataa ctcacgttaa gccttttcac cttgaaatca cttgttttcc
     3541 gtaaggtcct acagttgaag aaaattgaat ctataaatct gtgatttctt gcgtgacaaa
     3601 ctcgcacgat acgctttttg gattacctga actttaattc cccgatgagc actctcctgc
     3661 gaaaatattg catttgccag agctcagcgg cggtctcata accgtcttgg ttttgccatt
     3721 cagcttgcac ttgtccgtgc tctcggtggc acactgcgta cagaaaagct ccccccgaag
     3781 gctgttatca caatagtggc ggaacaactg ggtattgacc ttgccgtttt aaagcttaat
     3841 gtcacgaacg gtgcaaaagt ggttcttccg caacctgagt ggaagaaaat ggatgaaact
     3901 ttctggtgaa caaagatcta agctgtttta agttttgggc aactcaatgc actctcttaa
     3961 gacacttcta cagttacctt gcggatggcg atgcagtcga caaattatta gctctgacgg
     4021 tataaccctc catctccacg gaaaacgaaa aactgcacaa tgtcctgaat gctttaagcg
     4081 tagcgactct gttcatagtt gtcgtcggcg ccggatacag catctaccct gctaagggca
     4141 tacgctatgg cttgtatttt ccgtccgcca ctggtactgc cgtaacccgg cttgttcacg
     4201 taaaattttt gccgagtcgc ttgctccctt cgccagttca caccagcagt cttcacaggc
     4261 gttacaaaat ttacaacgtc aactgggatt aatagccgga ggtgaggccg ggaaacgggc
     4321 tgcaacggca gtgggtctcc gttgcagtgc tgatactctt cttcgcaggg ttatcaatac
     4381 cccggagacg aaacagtcag gcgcgcctca tgtcggtatt gatgagtggg cgtggcatcg
     4441 gggccaccgt tacagtacgt taatcgtcaa tcttgatact caccgtcccc tctcgtcctg
     4501 cttcccggcc gtgatcagcg tacgctggcg acctggttca gaaaatatcc ggaaatacag
     4561 gttgtctcgc gtgatcgcag tggagtctat gcgacagcag cacgtgaagg tgcacctcag
     4621 gccagacagg tggccgatcg atggcacctg ctaaaaaata ttggcgatgc gcctgaacga
     4681 atgatgtaca gacatatgcc tctgatacgt cttgttgcca gtgagttgtc accaaagaaa
     4741 tcacctgatc cagaaccata tgtgcctgca gcatcgctcc gtcgtccgga acacctaaaa
     4801 cagcaaaccc gcaaaaaacg gcatcagcgt tggacagagg ttatggccct gcatgacaag
     4861 ggatgtagtt tcagggaaat atcccgtatt acaggcctgt cgcgtgtgac agtcagtcgc
     4921 tgagtacgtt caggaacatt ccctgaaatg tcaacccgac ctccaaagcg agggcttttg
     4981 gacccatgga gggagtggtt aaaagagcaa cgagaaagtg ggaattataa cgccagccgg
     5041 ataatggcgg gaaatggtgg tccgggggtt tacaggcagt gaaaccattg tcagggatac
     5101 tgttgccaaa tggcgtaaag gctggatccc accggttact actgccgtca gacttccttc
     5161 agtgtcccgg gtaaggcgct ggttgatgcc ctggagaata atcagggatg aagaaaatta
     5221 tgcttcccga tttattagtc tgatgtgtga aaaagaaccg gggctgaaaa tagcgcagca
     5281 actggcactc gagttctacc gtattctgaa aacccaaaat aaatcacagc taagtagctg
     5341 gttcactcga gtccacgaaa gcggttcagc agaatttcgg cgcgtggcgg cggggatgga
     5401 ggctgatgct gcagctatat gtgaggcaat cagtagccgc tggagtaatg gtgttgtcga
     5461 aggtcatgta aatcgcctga agatgctaaa acgccagatg tatggtagag ccggatttga
     5521 actactcagg cagagggtca tgagtccact ggcatgaaaa tctccaccca agttgcggaa
     5581 gaaccacttt tgcaccgtta gtgacaggaa gaagaaatta cctcttcttc ggttctgaca
     5641 acggtggaga aagtgctgcg atcatctaca gtctgctggt tacctgcaaa ctgaatgggg
     5701 tggagccgga ggactggttg cgcgaagtga tcgtaaagat caataactgg tgctgccctg
     5761 taacttctct gctgcaaaat aatccttccg ctacgaactg ctcggggcgc ttacgtatat
     5821 ccggcctgtt catgaagtta ttgcttctgg ccttgatgag acattcgcgc gctccgacct
     5881 tatcggttac ttggccttac tttattttcc gctagtttta ctttcccatc gaaggcccca
     5941 tacatcacca cgcccacgaa aaaaccgacg aaataacgca actttctcat ccgtggataa
     6001 tacggaaaca cactgcacag gactctgcgg tttacgtcgc cattcaatcc cataagcttc
     6061 aagtgatgtc ttccctcagt gctgctaatt catcactgtc agatttatga accatattcg
     6121 ttacctgtat ccgaaccact gaagcaccac agcagaaatt cgggtcccct acactgatta
     6181 cagcgccccc tctactgcct attaactcaa gcacctccca acaacttacg gaaacttaat
     6241 tctaatacag ctgtaattca gttgtgtcgt ggtcggccgc ggttactttt ttcgggaagt
     6301 ctgctacgca tcagaataat accgtgttct ttctgatcct gtttagccag gtagctaagg
     6361 gcgtaacgta aaccgtctac cgcagactta tcactatagt gaatcacgtg atcgatacgc
     6421 accgggtatt tgtctttagt cctgcacagg tgaaaataac cctccccttc cgtgatccgc
     6481 ctccagatat ctcccagttg cctggatatc cgataagatt tcttgtgacg ttgcccatcc
     6541 aggtagccga taaaatgaat atgaagtccc ttgtttgacg tatattccat tacccagtaa
     6601 tatcccgcca gcatcgtttg ggtttcacta agtaagcggt gcatttcaat acacatactg
     6661 tgcctacagg aatgaccaaa actgggcgtg tctttcctgt aggcaaaatc aattctgaaa
     6721 ggtaacagtt tagaaaaacg ttgaaacaga ccttccatgt gttcattcac gtctttcaga
     6781 atcatgaagt ccatttcgta attgggatta gcattataca ttttatagtc cttactttat
     6841 aaaactgaca ggaaagtgga attacataaa tactgagagt acagaaacgc cgtaccgcgg
     6901 cattaataaa cgaggtaacc agcagcacag acaacctgat aaacctgttt cgtattacct
     6961 cattacagta atagggtaat gattcagaat acagtaagac tctcttaaac tgaggaccag
     7021 aacgaattac aataaaaaac acacctcata taattgggtt taagaaagca ccattcaatt
     7081 acttacagga aataatctga tcttcacaaa agtcagtacc gggtagccac aggcaacatc
     7141 gctattaata ttgccgtacc attaagtaaa aacagagact ctgcagagag agcctaca
//
LOCUS       DADTFG010000196         7186 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.197, whole genome shotgun sequence.
ACCESSION   DADTFG010000196 DADTFG010000000
VERSION     DADTFG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.197"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(2..94)
                     /locus_tag="J1138_004262"
     tRNA            complement(2..94)
                     /locus_tag="J1138_004262"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="J1138_004263"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="J1138_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBA8455973.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="J1138_004264"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="J1138_004264"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBA8455974.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3589..4089)
                     /gene="recX"
                     /locus_tag="J1138_004265"
     CDS             complement(3589..4089)
                     /gene="recX"
                     /locus_tag="J1138_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBA8455975.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4158..5219)
                     /gene="recA"
                     /locus_tag="J1138_004266"
     CDS             complement(4158..5219)
                     /gene="recA"
                     /locus_tag="J1138_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBA8455976.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5299..5796)
                     /gene="pncC"
                     /locus_tag="J1138_004267"
     CDS             complement(5299..5796)
                     /gene="pncC"
                     /locus_tag="J1138_004267"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBA8455977.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(5941..7026)
                     /gene="mltB"
                     /locus_tag="J1138_004268"
     CDS             complement(5941..7026)
                     /gene="mltB"
                     /locus_tag="J1138_004268"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBA8455978.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1675 a         2028 c         1729 g         1754 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atcaacagaa aaacggaggt ctgacagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggaataaagc gaaaagacaa tggagtgaaa
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatctaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtaactctt tttccagctg acgcgtacgt tccagtactg
     1261 aacgcacttt atcagccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggcga tagcgccttc tccggtcacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacgcgctc atcatacttc tcgccaaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca agttacggcg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tctggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcactttgtc gttaaccagt gaacctttct gcgatacatg agtacccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccta ctttcagaga acccgcagca agtttaccga
     1861 tgtggccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tgtcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcacgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccgtaggt gtcgtacagg cggaaagcag
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc agcgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cttgctggcg tttcaggtct tcacccgcag agcccatcac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattacc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggtttcgg cagcggttcc atcgtgccat cggcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccgcccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatccct acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac agagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgctaccagc cgttgcgtgt cgttaaccgc ccggctggta actgaaaagt
     3541 gggaataaga taagttttct tgactgggaa gtaaaatccc gtatgcgttc agtcggcaaa
     3601 atttcgccaa atatcctgga tatcttccat cagatagcca cgatagagca gaaaacgctg
     3661 gatcttaact ttttctgaaa agacagttgg caaaggttcg ccatattttc gcgtcgcctg
     3721 atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc atcgcttttt ctgtcgcttc
     3781 gcgggaaata cctttctgat tcagttcctg acgaatacgc gcaggtccat aacctttgcg
     3841 gctacggctg gcgataaagc gcgcaacaaa tcggctgtca tcaagatagc catgttcatg
     3901 gcaccaggca ataacgcgct cgtaatcttc tgccgtggca tcaatctctt ccgggccatt
     3961 tttgcccata atcggtgccg cgagtttacg tcgcagttct tgctcactgt gatcgcgcac
     4021 cgccagaatg cgtaccgcac gatccaacag gcgagcatat gccgggcgac gggatgttga
     4081 ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag ggccgcagat gcgacccttg
     4141 tgtatcaaac aagacgatta aaaatcttcg ttagtttctg ctacgccttc gctgtcatct
     4201 acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg
     4261 atctctttcg cggtttctgg gttatctttc agccaggcag tcgcattcgc tttaccctga
     4321 ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgataag cttctctttc
     4381 acgcccaggt caaccagctc gccgtagaag ttgatacctt cgccgtagag gatctggaat
     4441 tcagcctgtt taaacggcgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta
     4501 cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag acgaacagag
     4561 gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccaaa catcacacca
     4621 attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc
     4681 gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg
     4741 atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg
     4801 tctactgcgc cagaacgtgc cagggcgtca cagatttcca gtgcctgctc gccggtatcc
     4861 ggctgggagc acagcaggtt gtcgatatcg acgcccagtt tacgtgcgta gattgggtcc
     4921 agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cgcgctgcgc tgcggcgatc
     4981 acctgcaacg tcagcgtggt tttaccggaa gattctggtc cgtagatttc gacgatacgg
     5041 cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg
     5101 gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt
     5161 ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt
     5221 tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga agcaattata
     5281 ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc
     5341 atacgcagta gcctgacgac gcaccgcatc acggtcaccg ctgaagcatt cccgccgggt
     5401 aatgccttca ccacgggcag tggcaaaagc aaaccagacg gtgccgacag gcttctcttc
     5461 actgccgcca tccggcccgg cgataccact aatagacacg gcgtaatcag cacgagccgc
     5521 tttcagtgcg cctatcgcca tttccaccac gacgggttca ctcaccgcgc catgctgcgc
     5581 cagcgtctct tcgcgcacgc cgatcatctg cgctttggct tcgttactgt aggtgacaaa
     5641 tccgcgttca aaccaggcgg aactgccggc aatatcggta atcactttcg ctacccaacc
     5701 gccggtacaa gactcagcag ttgttacggt tgcgccacgg gctttcagcg cctgcccaac
     5761 ctgttcgctt aactgcatca gttcactgtc agtcatcaca acctctgtca gtcaaaaatt
     5821 tatgccggac aagataacac tttcgtcagg aagatggtgt tggcgtagca attttacgag
     5881 gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc ttgcaaatag
     5941 ttactgcacg cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg
     6001 ggtgctgtgg ttataacggg taatggtgta gaagttcggc agaccgtacc agtactggta
     6061 gccggtgcca acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg
     6121 tggcgttaaa cctgccgcgg caagctgcga aatgctgtac ctggttttga agccatttgg
     6181 caagcctggc gcctgaccgt ttgccattac cgcgacctga tcgcctttca cccagccgtg
     6241 cgctttgaaa taattcgcca cgctaccgat cgcatcaacc ggatcccaca ggttgatatg
     6301 cccgtcgccg ctgaaatcta ccgcgtattg tttgtaagac gacggcataa actgaccgta
     6361 gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc
     6421 catcagcagg aaggtttcca gctcgccaga aaaatactcc gcgcggcgtg ggtagttaaa
     6481 tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt
     6541 ttcaacgccg ataatcccga cgataatttc cggcggtaca ccatacacct gccacgcgcg
     6601 attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt tgtccggcgt
     6661 aataaatttt ttgcgataac gcagccatgc gccgttcgga cctgatggcg gtttcaccga
     6721 tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgtt tcgcctggga
     6781 gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa
     6841 ctgctgggca ttcgggttat tggcgaaatc accgcccatc tgcatcacat tgtgctgcgg
     6901 ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg
     6961 cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt
     7021 gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcccgcc
     7081 ccgcacaagg aagcggtagt cactgcccga tacggacttt acataactca actcattctc
     7141 ctcactacct tctttataca aactttcaaa ataaaatatt tatctt
//
LOCUS       DADTFG010000197         7027 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.198, whole genome shotgun sequence.
ACCESSION   DADTFG010000197 DADTFG010000000
VERSION     DADTFG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.198"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            533..1522
                     /locus_tag="J1138_004269"
     CDS             533..1522
                     /locus_tag="J1138_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8455979.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPGKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKASDIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1592..3106
                     /locus_tag="J1138_004270"
     CDS             1592..3106
                     /locus_tag="J1138_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HBA8455980.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3118..4107
                     /locus_tag="J1138_004271"
     CDS             3118..4107
                     /locus_tag="J1138_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HBA8455981.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4274..5074
                     /gene="otsB"
                     /locus_tag="J1138_004272"
     CDS             4274..5074
                     /gene="otsB"
                     /locus_tag="J1138_004272"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBA8455982.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQALWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5049..6473
                     /gene="otsA"
                     /locus_tag="J1138_004273"
     CDS             5049..6473
                     /gene="otsA"
                     /locus_tag="J1138_004273"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBA8455983.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6480..6908)
                     /gene="uspC"
                     /locus_tag="J1138_004274"
     CDS             complement(6480..6908)
                     /gene="uspC"
                     /locus_tag="J1138_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBA8455984.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
BASE COUNT         1774 a         1635 c         1825 g         1793 t
ORIGIN      
        1 aatcgatgaa tttgaattca tgatgaagca atgattttat aaaacgtttt ttcatttttg
       61 cgatatggct ctcattatac gtgttctgtt aattaatgag tacagtgata attaatggcg
      121 aaatgaatga cgcgcatttt ccacatcttt aagttgcggt tattacacca ttgcaaaaac
      181 tcaacgccag gtaatgaggc ctattgactg gttaaaaaga aaacatcccg catgggtacc
      241 aaagacaaca aggacttcca ggctaatctt atggattatt ctgctgtgca ctagataatt
      301 tgtctgacaa attcgctttt ccttatgtct tttcccgcta aatttatgca cgttctcact
      361 gtaattctgc gatgtgatat tgctctccta tggagaatta atttctcgct aaaactatgt
      421 caacacagtc acttatcttt tagttaaaag gtaatgcttt gttttccgat taatttaacg
      481 aatgtcattc gtttttgccc tacacaaaac gacactaaag ctggagagaa ccatgcacaa
      541 atttactaaa gccctggcag ccattggtct ggcagccgtt atgtcacaat ccgctatggc
      601 ggagaacctg aagctcggtt tcctggtgaa gcaaccggaa gagccgtggt tccagaccga
      661 atggaagttt gccgataaag ccgggaaaga tttagggttt gaagttatta agattgccgt
      721 gccggatggc gaaaaaacat tgaacgctat cgacagcctg gccgccagtg gcgcgaaagg
      781 tttcgttatt tgtactccgg acccgaaact cggctctgcc atcgtggcga aagcgcgtgg
      841 ctacgatatg aaagtcattg ccgtggatga ccagtttgtt aacgccaaag gtaagccaat
      901 ggataccgtt ccgctggtaa tgatggctgc gaccaaaatt ggcgaacgtc agggccagga
      961 actatataaa gagatgcaga aacgtggctg ggatgtcaaa gaaagtgcgg tgatggcgat
     1021 tacctccaac gaactggata cggctcgtcg ccgtactacg gggtctatgg atgcgctgaa
     1081 agctgcagga ttcccgggaa aacaaattta tcaggtacct accaaatcta acgacatccc
     1141 gggggcattt gacgctgcca actcaatgct ggttcaacat ccggaagtta aacattggct
     1201 gattgtcggt atgaacgaca gcaccgtgct gggcggcgta cgcgcgacgg aaggtcaggg
     1261 ctttaaagcg agcgatatca tcggtatcgg tattaacggt gtggatgcgg tgagtgaact
     1321 gtctaaggca caggcaaccg gcttctacgg ttccctgctg ccaagcccgg acgtacatgg
     1381 ttataagtcc agcgaaatgc tttacaactg ggttgcaaaa ggtgttgaac cgaccaaatt
     1441 caccgaagtt accgacgtgg tgctgatcac gcgtgacaac tttaaagaag aactggagaa
     1501 aaaaggttta ggcggtaagt aatttgccgg aaaaattccc ctctgcatga tgcagagggg
     1561 gtgtgaacga ccagtgattc acggagacgt tatgcaacag tctaccccgt atctctcatt
     1621 tcgcggcatc ggtaaaacgt ttcccggcgt taaggcgctg acggatatta gttttgactg
     1681 ctatgccggt caggttcatg cgttgatggg tgaaaatggc gcaggaaaat caactctctt
     1741 aaaaatcctc agcggaaact atgcgccaac cacgggttct gtagtgatta atgggcagga
     1801 aatgtccttt tccgacacga ccgcagcact taacgcgggg gtggcgatta tttaccagga
     1861 actgcatctc gtgccggaaa tgaccgtcgc ggaaaacatc tatctcggcc agctgccgca
     1921 taaaggcggc attgtgaatc gttcattgct gaattatgag gcaggtttac aaattaaaca
     1981 tcttggtatg gatattgacc cggacacgcc gctgaaatat ctctccattg gtcagtggca
     2041 gatggttgaa atcgccaaag cgctggcgcg taacgccaaa attatcgcct ttgatgagcc
     2101 aaccagctcc ctctctgccc gcgaaatcga caatcttttc cgcgttattc gtgaactgcg
     2161 aaaagagggg cgggtgatct tatacgtttc tcaccgtatg gaagaaatat ttgccctcag
     2221 cgatgccatc accgtcttta aagatgggcg ttatgtcaaa acctttaccg atatgcagca
     2281 ggttgaccac gacgcactgg tgcaggcgat ggtcgggcga gacattggcg atatctacgg
     2341 ctggcaaccg cgtagttatg gcgaggagcg gctgcgtctt gatgctgtga aagcaccagg
     2401 cgtgcgtacg ccaataagtc tggcggttcg cagtggagaa attgtcggtc tgtttggtct
     2461 ggtcggggca gggcgtagcg aattaatgaa aggcatgttt ggcggtacgc aaatcaccgc
     2521 cggtcaggtt tatatcgacc aacagccgat cgatattcgt aaaccgagcc acgctattac
     2581 cgcaggcatg atgctctgcc cggaagatcg caaagcggaa ggcattattc ctgtgcactc
     2641 cgttcgcgac aatatcaaca tcagtgccag acgcaaacat gtgctcggcg gttgtgtaat
     2701 caacaacggt tgggaagaaa acaatgccga tcaccacatt cgttcgctca acatcaaaac
     2761 gccgggcgct gagcaactga ttatgaatct ctcaggtgga aatcagcaaa aagccattct
     2821 gggccgctgg ttatcggaag agatgaaggt cattttgctg gatgaaccta cgcgcggcat
     2881 tgatgttggt gctaagcacg aaatatataa cgtgatttat gcgctggcgg cgcagggtgt
     2941 ggcggtgctg tttgcctcca gcgacttacc tgaagtcctc ggcgttgccg accggattgt
     3001 ggtgatgcgg gaaggcgaaa tcgccggtga attgttacac gagcaggcag atgagcgtca
     3061 ggcactgagc cttgcgatgc ctaaagtcag ccaggcagtt gcctgagtaa ggagagtatg
     3121 atgtcttctg tttctacatc gggttctggc gcacctaagt cgtcattcag cttcgggcgt
     3181 atctgggatc agtacggcat gctggtggtg tttgcggtgc tttttatcgc ctgtgccatt
     3241 tttgtcccaa attttgccac cttcattaat atgaaagggt tgggcctggc aatttccatg
     3301 tcggggatgg tggcttgtgg catgttgttc tgccttgctt ccggtgactt tgacctttct
     3361 gtcgcctccg taattgcctg tgcgggtgtc accacggcgg tggttattaa tctgactgaa
     3421 agcctgtgga ttggcgtggc agcggggttg ctgctgggca ttctctgtgg tctggtcaat
     3481 ggctttgtta tcgccaaact caaaataaat gccctgatca cgacactggc aacgatgcag
     3541 attgttcgag gtctggcgta catcatttca gacggtaaag cggtcggtat cgaagatgaa
     3601 agcttctttg cccttggtta cgccaactgg ttcggtctgc ctgcgccaat ctggctcacc
     3661 gtcgcgtgtc tgatcatctt tggcttgctg ctgaataaaa ccacctttgg tcgtaatacc
     3721 ctggcgattg gcgggaacga agaggctgcg cgtctggcgg gtgtaccggt tgttcgcacc
     3781 aaaattatta tttttgttct ctctggcctg gtatcagcaa tagccggaat tattctggct
     3841 tcacgtatga ccagtgggca gccaatgacg tcgattggtt atgagctgat tgtgatctcc
     3901 gcctgcgttt taggtggcgt ttctctgaaa ggcggcatcg gaaaaatctc atatgtggtg
     3961 gcgggtatct taattttagg caccgtggaa aacgccatga acctgcttaa tatttctcct
     4021 ttcgcacagt acgtagttcg cggcttaatc ctgctggcag cggtgatttt cgaccgttac
     4081 aagcaaaaag cgaaacgcac tgtctgatgc ttttttctac aacaatttag cgttttttcc
     4141 caccacagcc agccgccaca acggttggct gttcttcgtt gcaaatggcg acccccgtca
     4201 cactgtctat acttacatgt ctgtaaagcg cgttctgcgc aacacaataa gaaaagagaa
     4261 ggaggagaac cgggtgacag aaccgttaac cgaaacccct gaactatccg cgaaatatgc
     4321 ctggtttttt gatctcgatg gaacgctggc ggaaatcaaa ccgcatcccg atcaggtcgt
     4381 cgtgcctgac actattctgc aaggacttca gctactggca accgcaagtg atggtgcatt
     4441 ggcattgatt tcagggcgct caatggtgga gcttgacgca ctggcaaaac cttatcgctt
     4501 cccgttagcg ggcgtgcatg gggcggagcg ccgtgacatc aatggtaaaa cgcatatcgt
     4561 tcatctgccg gatgcgattg cgcgtgatat tagcgtgcaa ctgcatacgg tcatcgccca
     4621 gtatcccggc gcggagctgg aggcgaaagg gatggctttt gcgctgcatt atcgccaggc
     4681 tccgcagcat gaagacgcat taatgacatt agcgcaacgt attactcaga tctggccaca
     4741 aatggcgtta cagcagggaa agtgtgttgt cgagatcaaa ccgaaaggta ccagtaaagg
     4801 tgaggcgatt gctgccttta tgcaggaagc tccctttatc gggcgaacgc ccgtatttct
     4861 gggcgatgat ctgaccgatg aatctggctt cgcagtcgtt aaccggctgg gcggaatgtc
     4921 agtaaaaatt ggcacaggtg caactcaggc attatggcga ctggcgggcg tgccggatgt
     4981 ctggagctgg cttgaaatga taaccaccgc attacaacaa aaaagagaaa ataacaggag
     5041 tgatgactat gagtcgttta gtcgtagtat ctaaccggat tgcaccacca gacgagcacg
     5101 ccgccagtgc cggtggcctt gccgttggca tactgggggc actgaaagcc gcaggtggac
     5161 tgtggtttgg ctggagcggt gaaacaggga atgaggatca gccgctaaaa aaggtgaaaa
     5221 agggtaacat tacgtgggcc tcttttaacc tcagcgaaca ggaccttgac gaatactaca
     5281 acaaattctc caatgccgtt ctctggcccg cttttcatta tcggctcgat ctggtgcaat
     5341 ttcagcgtcc tgcctgggat ggctatctac gcgtaaatgc gttgctggca gataaattac
     5401 tgccgctgtt gcaagacgat gacattatct ggatccacga ttatcacctg ttgccctttg
     5461 cgcatgaatt acgcaaacgg ggcgtgaata atcgcattgg tttctttctg catattcctt
     5521 tcccgacacc ggaaatcttc aacgcgctgc cgacatatga caccttgctt gaacagcttt
     5581 gtgattatga tttgctgggt ttccagacgg agaacgatcg tctggcgttc ctggattgcc
     5641 tttctaacct gacccgcgtc acgacacgta gcgcaaaaag ccatacggcc tgcggcaaag
     5701 cgtttcgaac ggaagtttac ccgatcggca ttgagccgaa agaaatagcg aaacaggctg
     5761 ccggtccact gccgccaaaa ctggcgcaac tcaaagcaga actgaaaaac gtacaaaata
     5821 tcttttctgt cgaacgactg gattattcca aaggtttgcc ggagcgtttt ctcgcctatg
     5881 aagcgttact ggaaaaatat ccgcaacatc acggtaaaat tcgttatacc cagatcgcgc
     5941 caacgtctcg tggtgatgtg caagcctatc aggatattcg tcatcagctc gaaaatgaag
     6001 ccggacgaat taatggtaaa tatgggcaat taggctggac gccgctttat tatttgaatc
     6061 agcattttga ccgtaaatta ctgatgaaaa tattccgcta ctctgacgtg ggcttagtga
     6121 cgccactgcg tgacgggatg aacctggtgg caaaagagta tgttgccgct caggacccag
     6181 ccaacccggg cgttcttgtt ctttcgcaat ttgcgggagc ggcaaacgag ttaacgtcgg
     6241 cgttaattgt taatccctac gatcgtgacg aagttgcagc tgcgctggat cgtgcattga
     6301 ctatgtcgct ggcggaacgt atttcccgtc atgcagaaat gctggacgtt atcgtgaaaa
     6361 acgatattaa ccactggcag gagtgcttca ttagcgacct aaagcagata gttccgcgaa
     6421 gcgcggaaag ccagcagcgc gataaagttg ctacctttcc taagcttgcg taggtgcggt
     6481 taatctcccg taagtggaac taacagcaca tcgacctcac ttgaggtaat cactcttttc
     6541 gccgagcagg atgctcgcga aaagaaactg tgattgtgat taccgcaaat caccaaatcg
     6601 aaatgatgtt tgcgacaaac ttccagaata tgttcgctta actcaccgta ggcaataaac
     6661 gtcttgtcaa cgggataccc cgcatcctga attaacttat caagaaagct ttgcgtttct
     6721 tcctgcatca cattgcgtaa atcttcaagc atcggcgcag ctaattgatt gtacatttcc
     6781 gggtcggaag cgagagtaat taaactgatg tgtcccttta ctggcctggc gatagatacc
     6841 gcttttgcca gcagttgctg actttccggt gtaaccgcaa cggcgacaag aatattgcta
     6901 tagctcataa cctgttcctt attctgtgaa cttcaggtga cattaaagct ttactcctaa
     6961 tggagaaacg acgcaatccc aactcagtta aacaggtaaa tattagtctg gtcattagta
     7021 aatacaa
//
LOCUS       DADTFG010000198         6927 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.199, whole genome shotgun sequence.
ACCESSION   DADTFG010000198 DADTFG010000000
VERSION     DADTFG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.199"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..947)
                     /gene="dsdC"
                     /locus_tag="J1138_004275"
     CDS             complement(<1..947)
                     /gene="dsdC"
                     /locus_tag="J1138_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HBA8455985.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLAHHFLMDEEILPVCSPEYAQRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVSQYECYTL"
     gene            1165..2502
                     /gene="dsdX"
                     /locus_tag="J1138_004276"
     CDS             1165..2502
                     /gene="dsdX"
                     /locus_tag="J1138_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HBA8455986.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMVPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTVLLPIALMLVKTIAELNMARESGLYTLLEFIGNPITATFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVIALAGTFLLSFII"
     gene            2520..3848
                     /gene="dsdA"
                     /locus_tag="J1138_004277"
     CDS             2520..3848
                     /gene="dsdA"
                     /locus_tag="J1138_004277"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBA8455987.1"
                     /translation="MENAKMNSLIAQYPLVEDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAASGGIIESELVAIPAMQKRLEKEYHQPIAGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYGMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(3956..5494)
                     /gene="emrY"
                     /locus_tag="J1138_004278"
     CDS             complement(3956..5494)
                     /gene="emrY"
                     /locus_tag="J1138_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBA8455988.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5494..6657)
                     /gene="emrK"
                     /locus_tag="J1138_004279"
     CDS             complement(5494..6657)
                     /gene="emrK"
                     /locus_tag="J1138_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBA8455989.1"
                     /translation="MEQVNSNKKHSDRRKYFALLAVVLFIAFSGAYAYWSMELKDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1848 a         1583 c         1568 g         1928 t
ORIGIN      
        1 tataaggtat aacattcata ttgacttacc tgttctctta accaagtaat aaatgcctca
       61 atttttggcc actgcctgcc cggtaatgtg gtgatgtaat aatgctgatg gcatttcacc
      121 gtcatatcgc caaacggcgc gacgagctca ccactggcaa gccttttttg taccagacgt
      181 ttccttccca tcgccacccc aatatgattc atcgcggcga taacggctaa atcagagcga
      241 tcaaagccaa ttccagaaga tgttggcaaa ttaaccgcat aatgttgcgc ccaactatgc
      301 cattcatccg taccggagtc gttgctccat gcctgtctgt catggagcaa cgtacagtga
      361 cgcaggttaa ttaccgtgtt ggttaaatca tgtctttgag cgtactccgg gctgcaaact
      421 ggcaagattt cttcatccat cagaaagtga tgagccaatt gcgctgacgg cgcatcatca
      481 aagtagatcg ccaaatcgat tccggcacgt tgcaaattga cattgtcatt accagtgagc
      541 acggtgagcg aaatagacgg atatcggcgt gtaaagtcac ctaatgcggg caccaaccag
      601 cattgggcga tagagggccg ggaatacagc gttaacgttc ccgataactc ctgatttttg
      661 atatccagaa tttcctgatt cagggtatcc agcgacgatt ttagcgccca ataaacacgt
      721 ttcccctcgt gcgttaattc cactttgcga tgggaacgaa caaacaactg aatacctaat
      781 tcttcttcca gctgattgat acggtgactt accgcactgg ggctcagcga caattcctct
      841 gccgcgagag cgaaggactg atgcctggca gccacttcaa aagtatgcag ttttgatagt
      901 tgccagccgt taagcagtcg atttcttatt tcacgaaggg gttccataat cacctcattt
      961 ttctcttaag tgtaaaaaaa tagcggcaaa atttcagctg taaggtgagc taaagtgaac
     1021 catatctcaa ttcaccttca tttttagatg taaatcactc ccttgatgca atttagctca
     1081 tgtgaaaggc aaattttatc gtttgtcagc ctgcgttgtt tttttgtcca atatcatcag
     1141 gttaatcaca ggggaaggtg agatatgcac tctcaaatct gggttgtgag cacgctgctt
     1201 atcagcatcg tgttaattgt actgaccatc gtgaagttca aattccaccc gtttctggcg
     1261 ctgttgctgg ccagcttctt cgtgggaacg atgatgggca tggtgccact ggatatggta
     1321 aatgctattg aaagtggaat tggcggaacg ctggggttcc tcgctgcggt tatcggcctt
     1381 ggcacgatac tgggaaaaat gatggaagta tccggggccg cagaaagaat tggtctgaca
     1441 cttcaacgct gccggtggct ttcagctgat gtcattatgg tgctggttgg cctgatttgc
     1501 ggcatcacgc tgtttgttga agtgggcgtc gtgctattga ttcctctggc tttttcaatt
     1561 gccaaaaaaa ccaatacctc attgttaaag ctggccattc cgctgtgtac cgcattgatg
     1621 gcagtgcact gcgtggttcc cccacatccg gctgctttat atgttgccaa taagctgggc
     1681 gcagatatcg gttcggtgat cgtctacggt ttgctggttg ggctgatggc atcactgatc
     1741 ggtggcccac ttttccttaa atttctgggt caacgactgc cctttaaacc tgtacccaca
     1801 gagtttgccg atctcaaagt tcgcgatgaa aaaacactac cgtcattagg cgcaacgtta
     1861 ttcaccgtac tgctacccat tgcgctgatg ttggttaaaa cgattgccga attgaatatg
     1921 gcacgtgaga gtggtttgta taccttgctt gagtttattg gcaaccctat cactgccacg
     1981 tttatcgccg tgtttgtcgc ctattatgtg ttgggtatac gtcagcatat gagcatgggg
     2041 acgatgctca cacatacgga aaatggtttc ggttctattg ctaatatttt gctgattatc
     2101 ggggccggag gcgcattcaa cgccatttta aaaagcagca gtctcgctga tacgctggca
     2161 gttattctct ccaatatgca tatgcacccg attcttctgg cctggttggt ggcacttatt
     2221 ctgcatgctg cagtgggctc cgctacggtg gcaatgatgg gggcaacggc aattgttgca
     2281 cccatgctgc cgctgtatcc cgacatcagc ccggaaatta ttgcgattgc tatcggttcc
     2341 ggtgcgattg gctgcacgat cgttacggac tcacttttct ggctggtgaa gcaatattgc
     2401 ggcgctacgc tcaatgaaac atttaaatac tatacgacag cgacatttat cgcttcagtc
     2461 atcgctctgg cgggcacatt cctgctgtca tttatcatct aagcgcaaag agacgtacta
     2521 tggaaaacgc taaaatgaat tcgctcatcg cccagtatcc gttggtagag gatctggttg
     2581 ctcttaaaga aaccacctgg tttaatcctg gcacgacctc attggcagaa ggtttacctt
     2641 atgttggcct gaccgaacag gatgttcagg acgcccatgc gcgcctgtct cgttttgcgc
     2701 cgtatctggc aaaagcattt cctgaaacgg ctgcctcggg ggggattatt gaatcagaac
     2761 tggttgctat tcctgctatg caaaaacggc tggaaaagga atatcaccag ccgatcgccg
     2821 ggcaattgct actaaaaaaa gacagccatt tgccgatttc cggctccata aaagcacgcg
     2881 gcgggattta tgaagtcctg gcacatgcag aaaaactggc tctggaagcg gggttgctga
     2941 cgcttgaaga tgactacagc aaactgcttt ccccggagtt taaacagttc tttagccaat
     3001 acagtattgc tgtgggatca accggaaatc tggggttatc aattggtatt atgagcgccc
     3061 gcatcggctt taaagtaacg gtgcatatgt ctgctgatgc ccgcgcctgg aaaaaagcga
     3121 aactgcgcag tcatggcgtt actgtcgtgg aatacgagca agattatggt gttgccgtcg
     3181 aggaaggacg taaagccgcg cagtctgacc cgaactgttt ctttattgat gacgaaaatt
     3241 cccgcacgtt gttccttgga tattccgtcg caggccagcg tcttaaggcg caatttgctc
     3301 agcaaggtcg catcgtcgat gctgataacc ctctgtttgt ctatctgccg tgtggtgttg
     3361 gcggtggtcc tggtggcgtc gcattcggac ttaaactggc gtttggcgat catgttcact
     3421 gcttttttgc cgaaccaaca cactccccgt gtatgttatt aggcgtccat accggattac
     3481 acgatcagat ttctgttcag gatattggca tcgacaacct taccgctgcc gatggccttg
     3541 cagttggtcg cgcatcgggc tttgtcgggc gggcaatgga gcgtctgctg gatggtttct
     3601 atacccttag cgatcaaacc atgtatggca tgcttggctg gctggcgcag gaagaaggca
     3661 ttcgtcttga accttcagca ctggcgggta tggcggggcc tcagcgcgtg tgcgcatcag
     3721 taagttatca acagatgcac ggtttcagcg ccgaacaact gcgtaatgcc actcatctgg
     3781 tgtgggcgac gggaggtgga atggtgccgg aagaagagat gaatcaatat ctggcaaaag
     3841 gccgttaata acgtttcaac gcagcatcac catcctttcc ctgggtgagc gatgctgcgg
     3901 atgacgcaga cttaagattc tgccgtttta cctgctatag tctcctcttt ttccatcacc
     3961 caacgccttt cgctgtaaac ggcggtttcg caaaccaaac caaaaccgta agtaagataa
     4021 aagcaatagc cgccatacgg aaaatctcat ttgcagaaat agaaagtgac tgctgagtta
     4081 tttcattatt aatttcatta agaactcctg aaagcgaacc atagtattta tccataattt
     4141 gtgacgatga attaaacacg gggttaaatt gatcgatggt tgctgtcaac tgactatggt
     4201 gtaacgattc gcggcgtccc cacagtgtca ttgtcaacga cgtaccaact gatcctgaca
     4261 aggtgcgaaa aaaattactc atactcgagg cattggcaaa tttattatct ggcaagcctg
     4321 aaaacgaaat cgttgttaag ggtaaaaaga aacaggcaac ggcgaatccc tgaaaaaact
     4381 gtggcaaaat gatgcctgta aaatcaatcg ttggcataaa tgtcacagaa cgccagtaat
     4441 agcaaaccgc atacatcaaa aaactaaatg tcaccaacaa ccgcatatca attttgttgc
     4501 cataacgtcc tatcaaaggt gatattaata gcggcatgat accgatgggc gcataggcaa
     4561 gtccggccca tatcgcatta taccccatcg tttcctggag taactgcggc ataagaacta
     4621 tcgctccaga gtaaaataaa tacgcgcatg tgatactcac aataccaatg gtgaaattac
     4681 gggacttaaa caaactgagg tcaagaatcg gattctctga ggtcgactcc caaatgacta
     4741 aagaaatcag agaaataact gatactactg ttagtagtat tattgtactc gagttgaacc
     4801 aatccagatc gcgcccttta tcaagcataa tttgcaagcc accaacaccg agcactaaca
     4861 gggtcagtcc tggtagattc attttgaccg gtgaagtctc agtttctcgt cctttaagta
     4921 aggttaagca taatgtcagg acgataatcc ccatagggac attgattaaa aatatccaac
     4981 cccagctaaa gttatcgcaa atatagccgc ccaatatcgg cccacatatc ggagcgataa
     5041 tcacggtcat tgaccataat gccagagcaa atgttctttt ttctggtgga taattcctta
     5101 ataataaact ctgtgacagt ggaattaacg gccccgccat taacccctga acgactctaa
     5161 aaaatatcag cacatcaaga ttggtcgata aactacacat taatgaagac agcgaaaaaa
     5221 aggttactga aagtaaaaat aaccttaatt cgcctattct ttgtgccagc ctgccggtaa
     5281 cagggatcgc aatggcattt gctacgccaa acgaggtgat aacccaggtg ccttcgtctg
     5341 ttgatgctcc cagaaagcca gatattgtcg gtattgcgac gttagaaata gtggaatcca
     5401 acatttgcat aaacgtcgct aatgacaatg caatagtgac gcaccataac gtcccaccgg
     5461 ttaatggtgc aggagttgat ttagtgattg ccattaaagt tgtccattat gcgaaataat
     5521 gttgctaatt tctttttcta tcggactggt atcgataact aaagccttac tggtataagc
     5581 aggcatggac gtcacggttg aagccagatc gggcatctcg gcaatgtctt cattcttcgt
     5641 atcaatagta gcagtcatcg ataaaccaat gcgcaaaggg tgttccatga gttcttttgg
     5701 atccagagaa acttcaaccg gtacacgctg aacgattttg atccagttcc ctgtcgcatt
     5761 ttgtgcaggt aataaggaga acgcattgcc ggttcccata ttgatccctg tcacccgacc
     5821 atgaaacaca acattttcac cataaagatc gctgataata ttgaccgatt gaccaatccg
     5881 tacatccgtg agttgtgttt ctttaaagtt ggcattaacc cacatttgac gcgccggtac
     5941 gacagccatt aacgattgtc cgggactcac tgtttcgccg acctgaacac ttctctgggc
     6001 aatataaccg gtaaccggac tcttaatatc cgtacgttta agcgccaacc aggcttcttt
     6061 ggttgcatct gcagcttcaa tgacttgtgg ctgacggttt aatggtgtgt tcattactaa
     6121 agctttattc gctttataag cctggatagc agcattcaat gccgctttgc tacttattaa
     6181 cgtatctttg gtatgctcca gcgcttcttt tgaaataacc ccttgcttcg ctaaaggcac
     6241 acgacggtta taatcttcta aagattgttg atactgaata cgtgctgaag cgacttcggc
     6301 actgtattgt ttatcctgta agtatagttt attcgtttgc cgaacaatat ttgccagatt
     6361 atttttagct ttattgagtg cgatagtggc atcagtttta tccagtgaaa ctaaaatgtc
     6421 accttgtcga acgtagttcg tatctttatg attaacgaca gtgacactgc ctgagacttg
     6481 tgcagaaatt ggatctgcat ttcccgtgac ataggcgtca tctgtactaa tcatgtcttt
     6541 taattccatt gaccaatagg cataggcacc tgaaaacgca ataaataaaa ctaccgctaa
     6601 caaagcaaaa tactttcttc tgtcagaatg ttttttattt gaattaacct gttccactat
     6661 tatctctcat ttcgcataga tgaaatttat gaattgaatg aaaagagtat tcattcctca
     6721 tatcaaacac agtgcatgtt ttgcacatta tcccgggaac gttggagggc taatttaaat
     6781 gaagtgcaaa aaaaacaaca gaggactaaa ccgcggcttt tgcaatacaa ttcttacgcc
     6841 tgtaggatta gtgagaaaac ttatagtgct catttgaaac tataaatcat cggtatcatc
     6901 cctgatttta ttgttgacat tttattt
//
LOCUS       DADTFG010000199         6793 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.200, whole genome shotgun sequence.
ACCESSION   DADTFG010000199 DADTFG010000000
VERSION     DADTFG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.200"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            62..625
                     /locus_tag="J1138_004280"
     CDS             62..625
                     /locus_tag="J1138_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBA8455990.1"
                     /translation="MLKKTLLSMFATALLSGVAFNALADDANQGSGKITFKGEVIDAP
                     CSIAPGDEDQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSS
                     ASVDATDSNLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNF
                     KARMESLGRTATPGNVQAQANYVLDYK"
     gene            712..3234
                     /locus_tag="J1138_004281"
     CDS             712..3234
                     /locus_tag="J1138_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBA8455991.1"
                     /translation="MNNKNTFSRDKLSHAIKNALSGVVSSLLFVLPVHAVEFNVDMID
                     AEDRENIDISRFEKKGYIPPGRYLARVQINKNMLPQTLILEWVKADNESGSLLCLTKE
                     NLTNFGLNTEFIESLQNIAGSECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDAGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTVQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQQQNSGNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSTSNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYGDNAR
                     MMVDTDGISGIEINSNRTVTNGLGIAVIPSLSNYTTSMLRVNNNDLPEGVDVENSVIS
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSTLHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3250..3990
                     /locus_tag="J1138_004282"
     CDS             3250..3990
                     /locus_tag="J1138_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA8455992.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRDYISTLPPMERIEPDEQIQIRLMALASLNDLPQD
                     RETLFYYNVREIPPRAKEQNVMQIAMQSRLKLFWRPKAIELKEGEMIPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVAPFTSATQHLSSLPSTFQLGFVAD
                     YGGLEMFKVECNSIQSLCQSSPAKKGKI"
     gene            3987..5036
                     /locus_tag="J1138_004283"
     CDS             3987..5036
                     /locus_tag="J1138_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HBA8455993.1"
                     /translation="MITLFRLLAILCLFFNVSAFAVDCYQDGYRGTTLINGDLPAFKI
                     PENAQPGQKIWESGDINITVYCDNAPGWTTDNPSENIYAWIKLPQINSADMLNNPYLT
                     FGVTYNGVDYEGINERIDTHACLDKYEQYYNGYYYDPVCNGSTLQKNVTFNAHFRVYV
                     KFKSRPAGDQTVNFGIVNVLQFDGEGGANMSSNAKNLRYVITGLDNISFLDCSVDVRI
                     FPESQIVNFGQIAANSIATYRPKAAFSVSTIKDVAADCTEQFDVATSFYTTDTLHDDT
                     HLEMGNGLLMRITDQKTQEDIKFNQYKRFTTYIPGQTGAIVTRDYQAELSQKPGETLV
                     YGPFKKDLIVKINYN"
     gene            complement(5091..5744)
                     /gene="ribB"
                     /locus_tag="J1138_004284"
     CDS             complement(5091..5744)
                     /gene="ribB"
                     /locus_tag="J1138_004284"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBA8455994.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5848..5996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6118..6417
                     /locus_tag="J1138_004285"
     CDS             6118..6417
                     /locus_tag="J1138_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HBA8455995.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPVAELQSPPAIPPVDKAE"
     gene            complement(6452..6652)
                     /gene="glgS"
                     /locus_tag="J1138_004286"
     CDS             complement(6452..6652)
                     /gene="glgS"
                     /locus_tag="J1138_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBA8455996.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         1941 a         1631 c         1492 g         1729 t
ORIGIN      
        1 gctgaattaa tattaaattc aaacctgtat tttcctcatc catttataaa ggattatagt
       61 catgttaaaa aaaacattgt tatctatgtt cgcaaccgca ttgttatcag gcgttgcttt
      121 taacgctctt gctgacgatg ctaatcaggg ttcaggtaaa attactttta aaggtgaagt
      181 tatcgatgca ccttgttcta ttgctcctgg tgatgaagat cagacaataa acctcggtga
      241 agttgctgat accgtattaa aaagcggtca gaaatcactg cctgtagatg tcaccattca
      301 tttgcaggat tgtattttat ctgacggcac taacactgtt gataaagtca aaatcacctt
      361 tagttctgcc agtgttgacg ctaccgactc caacctgctt aaaaacactc tggaaggtaa
      421 catcggcggc gcaactgatg taggcgtacg tctggtgaaa tcagacaaca ccaacgtgac
      481 tcttggcact ccaatcacta tcaacttccc gacgactaac tcttaccagg agttgaactt
      541 taaagcccgt atggagtctc tgggacgcac cgcgaccccg ggtaacgtgc aggcacaggc
      601 taattacgta ctcgactaca agtaatcggt agtcaatacc tgcacaacgc caggcggtaa
      661 agccgatgat atttcatcgg caactttggg cctttttaga aatggatttt tatgaacaat
      721 aaaaacacgt tttcccggga taagttatcc cacgcaatta aaaatgccct gtctggcgtt
      781 gtgagttccc tactcttcgt tttgccagtc cacgccgtag aattcaacgt cgatatgatt
      841 gacgcagaag accgtgagaa tatcgacatc tctcgttttg agaaaaaagg ctatatcccc
      901 cctggtagat acctcgctcg tgtgcaaata aataaaaata tgttgccaca aacgttaata
      961 ctggaatggg taaaagccga taatgaaagt ggttcgttac tctgcttaac caaagaaaat
     1021 ttgactaatt tcggtcttaa tacggaattt attgaatcat tgcaaaacat agctggcagc
     1081 gaatgtctcg atttaagcca acgtcaggag ttaacgacac gacttgataa agctacgatg
     1141 atattatcgc taagtgttcc ccaggcatgg ttaaaatacc aggcaacaaa ctggacgcca
     1201 ccagagtttt gggatgccgg tatcgccggg tttatccttg attacaacgt gtacgccagc
     1261 caatatgccc cacatcatgg agacagcacc caaaacgtca gctcctatgg tacgttaggc
     1321 tttaacctcg gcgcatggcg cttacgtagc gattaccaat ataatcagaa ttttgctgat
     1381 ggacgctcgg taaaccgcga cagcgaattt gcgcgaactt atctgtttcg ccctatcccc
     1441 tcctggtcgt caaaattcac tatgggccag tacgacctga gctccaatct ttacgatacc
     1501 ttccacttta ctggcgcatc gctggaaagt gatgaaagca tgctgccgcc agatttacag
     1561 ggttatgcgc cacaaattac cggcatcgcg cagaccaacg cgaaagtaac tgtggcacaa
     1621 aatggtcgtg tactttatca aaccactgtc gcgccaggcc cttttactat ttctgatttg
     1681 gggcaatcgt ttcaggggca gctggatgtc acagtggaag aagaagatgg ccgcaccagc
     1741 accgtccagg ttggctccgc atccattccc tatttaaccc gtaaagggca agtgcgctat
     1801 aaaacgtcac tgggaaaacc gacatccgtc gggcataacg atatcaataa tccctttttc
     1861 tggacggcgg aagcctcctg gggctggctg aacaatgtgt cgttgtatgg tggtggcatg
     1921 ttcaccgctg atgattatca ggctatcact accggtattg gctttaacct taaccaattc
     1981 ggttcgcttt cttttgatgt caccggagca gacgcgtctt tacagcaaca aaatagcggc
     2041 aatctgcgtg gttacagcta tcgcttcaac tatgcaaagc atttcgaatc gacaggcagt
     2101 cagattacct tcgcgggtta tcgcttctca gataaagatt acgtgtcgat gagtgagtac
     2161 ctcagctcgc gtaatggcga tgagtcaatc gataatgaaa aagagagtta tgtcatttcc
     2221 ttgaaccagt actttgaaac gctggaatta aactcttatc tcaacgttac acgcaatact
     2281 tattgggaca gcaccagcaa taccaactac tccgtatctg taagcaaaaa ctttgatatt
     2341 ggcgatttca aaggtatatc tgcatcgctg gcagtaagtc gaatccgctg ggatgacgac
     2401 gaagagaatc aatattactt ctctttctct ctacctttac aacaaaaccg caacatctcc
     2461 tacagtatgc agcgaacggg aagcagtaat acttcgcaga tgatttcctg gtacgattca
     2521 tcagatcgca acaatatctg gaatatttca gcgtcggcaa cggacgacaa tatacgtgat
     2581 ggcgaaccaa cactgcgcgg cagctaccag cactattcgc cgtggggacg tctgaacatt
     2641 aatggcagtg tacagccgaa tcagtacaat tctgttaccg caggctggta cggttcactt
     2701 accgctacac gtcatggtgt cgcccttcac gattatagct atggcgataa cgcccgcatg
     2761 atggtcgata ccgatggcat ctccggcatt gaaatcaact ctaaccgtac cgttaccaac
     2821 gggctgggca tcgccgtgat accttcgtta tcgaactaca ccacctccat gttgcgggtg
     2881 aacaataacg atctgccaga aggtgtcgat gtcgaaaact cggttattag taccacgctc
     2941 acccagggtg ccatcggcta cgcaaaactg aatgccacca ccggttacca aatcgtcggc
     3001 gttattcgtc aggaaaatgg gcgcttccct ccactgggtg tgaatgtcac ggataaagcg
     3061 acaggtaaag atgtgggcct ggtcgcggaa gacggcttcg tttatctcag cggtattcag
     3121 gaaaatagta ctctgcattt aacctggggt gataatacct gtgaagtcac gccgccaaac
     3181 caaagtaaca ttagcgaaag cgcaataatt ttaccttgta aaacagtcaa ataattcagg
     3241 atgaatataa tgaaatcacc attaatcaca acaggaatga tttgtttgct ggggatctgc
     3301 aactttgccc aggcgactgt ctcccccgac agaacacgca tcatttttaa tgcttcgaat
     3361 aaaagcgcaa cggttcgcct gaccaaccaa agtaaaatcg atccctatct ggctcagtcg
     3421 tggattgaag atgccagcgg taaaaaaacg cgtgattaca tctcgacatt gccgccgatg
     3481 gaacgtattg agcctgatga acagatacaa attcgcctga tggcactggc ctcgttaaac
     3541 gacttaccac aggaccgaga aacgttgttc tactataacg tgcgagaaat tccgccgcgc
     3601 gccaaagaac agaatgtaat gcaaatcgcc atgcaaagcc gcctgaagct attctggcgg
     3661 ccaaaagcga ttgagttaaa agagggggag atgattcctc tgcaaaaagt aacgattacc
     3721 cgcaccgccg ccggtctgac cctgaataac ccgacgcctt atcatattac cgtcggttat
     3781 atcggcacta atggcaagac actgatgccc ggtgcggaca gtattatggt ggcaccattt
     3841 acttctgcaa ctcaacacct aagcagcctg ccctctacct tccagttagg ctttgtggcg
     3901 gactacggcg ggctggagat gtttaaagtc gaatgtaatt ccatccaatc cctctgccaa
     3961 agctctcctg ctaagaaggg gaaaatatga ttacgctttt tcgtttactg gcgattcttt
     4021 gcctgttttt taacgtttca gcttttgctg ttgattgcta tcaggatggg tacagaggaa
     4081 caaccctcat aaatggagat ttaccagcgt tcaaaattcc agagaacgcg caacctgggc
     4141 aaaaaatttg ggagagtgga gatattaata tcacggttta ttgtgacaat gcaccaggat
     4201 ggacaacaga caatccatca gaaaatatct atgcctggat caaattaccc caaataaata
     4261 gtgccgacat gttgaataat ccatatttaa cctttggcgt gacttataat ggtgtagatt
     4321 atgaagggat aaatgaaaga attgatactc atgcatgcct ggataaatat gagcaatact
     4381 ataatgggta ttattatgac cctgtatgca atggcagtac ccttcaaaaa aatgtaacat
     4441 ttaatgccca ttttcgcgtc tatgtaaaat tcaaaagccg cccagcagga gatcagacgg
     4501 tgaactttgg catagtcaac gtgctacaat tcgatggcga aggaggggca aacatgtcct
     4561 ctaacgcgaa gaatttacgc tatgtgatta cgggattaga taatatttca ttccttgact
     4621 gtagtgtcga cgtccggatt tttccagaaa gccagatagt taatttcggg caaattgctg
     4681 caaactctat tgctacttat cgaccaaaag ctgcatttag cgtatctact ataaaagatg
     4741 ttgctgccga ttgtaccgag caatttgatg ttgccaccag cttttatacg acagatacgt
     4801 tacatgatga tacgcatctg gaaatgggta atggtttgtt gatgcgtatt actgatcaaa
     4861 aaacacaaga agacattaag tttaatcagt acaaacgctt caccacatat attccaggtc
     4921 agactggagc gatagttacc cgtgattacc aggctgaact ttcacaaaaa ccgggggaga
     4981 ctctcgttta tgggcctttc aaaaaagatc tcatcgtaaa aataaactat aactgaacag
     5041 caaaatgccg ggaacaaccc cggcattttc atatttagta tttcacgtca ttagctggct
     5101 ttacgctcat gtgcctgacg gtatgccacc agatcttcaa tagtcacgag cgccatattg
     5161 tgtttattgg caaactcaat gcactctggt gcacgcgcca tcgtgccatc gtcattagtc
     5221 agctcacaca gcacaccagc tggtttaaag cctgccagcg tcatcagatc gatagtcgct
     5281 tcagtatgac cgccacgcgt cagtacacca cctgcctgcg cgcgaagtgg gaaaacgtgg
     5341 cctgggcgat tcagatctga aggttttgcg ccatcggcaa tcgctgcgcg aacggtcgta
     5401 atacggtcag cggcagaaac accggtagtc acaccttcag ctgcttcaat ggtcacggta
     5461 aaaccggtgc cataggcgct ggtgttattt tctaccatca ttggcagatc gagttgttta
     5521 cggcgatcgt cagtaatgca caggcaaaca ataccactcc cgtggcgaat ggtcagagcc
     5581 atctgctcaa cagtcatggt ttctgccggg aagatcatat cgccttcgtt ttcacggtct
     5641 tcatcatcaa gcaccatcac accgcgtcct tcacgcagcg cagccagtgc attttcaaca
     5701 cgttcgaaag gcgtaccaaa agaggaaagt agcgtctgat tcatggtaaa aaaacctcac
     5761 taaaattatg gttaccagaa tcagggcagt cttaggagtg gcggcatata gccaaaataa
     5821 cgtgagcggg tccatgcccg acagaatcgt tactctctcc catccggact ctaaccgtcg
     5881 gccccggaat tacaccggat ctgctgtcct ttgagttcgc acccaaagcg ctcgcgggct
     5941 ttcaactgag ttgatttacc gccggtgggg aatttcgccc cgccctgaga ataagcggat
     6001 tcactataac gctaatgatt agcgccagca acgcatagct tcacataatt ctggtttatg
     6061 acttaccctt atcgcactac aatggcactc aacacctatc agtacaggga aactacaatg
     6121 attgacccga aaaaaattga gcaaatcgct cgccaggttc acgaatcaat gcctaaagga
     6181 atcagggagt tcggggaaga tgtggagaaa aaaatccgcc aaaccctaca agcgcagctg
     6241 acgcgtctcg atctggtaag ccgcgaagaa ttcgacgttc agacgcaagt cctgttacgg
     6301 actcgtgaaa aactggcgtt actagagcag cgcatcagcg aactggaagc gcgcaataat
     6361 ccggtagcag aacttcaatc tcctccggct atcccgccag ttgataaagc agaataaagc
     6421 accacaaaaa cgggccggac ggcccgtaat atcagtgttc taattcaagt tctctggttt
     6481 gcatcatctg ttggcaatac caggcataac gtgcgcaaaa ccaggttcta tgttcttcat
     6541 ccatgttacc agtaacggcc agaatatcga ccgggcgacc ttctgcgtgc attctggcaa
     6601 aactacgcgc gagaaaatcg aaattattta aagaattaag actatgatcc ataagcacat
     6661 cctccggtta ttgcatacct tttaaggcca tatgcttttt ctgatttcag tattccccaa
     6721 tcagtgtagt gcctttaaac ataacgtgcg taaatatatt gtccccgatc acacttttta
     6781 gtgaaaaata aaa
//
LOCUS       DADTFG010000200         6760 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.201, whole genome shotgun sequence.
ACCESSION   DADTFG010000200 DADTFG010000000
VERSION     DADTFG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.201"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            178..1458
                     /gene="wcaK"
                     /locus_tag="J1138_004287"
     CDS             178..1458
                     /gene="wcaK"
                     /locus_tag="J1138_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBA8455997.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQMGMRMVQSVLERIGEAK"
     gene            1455..2675
                     /gene="wcaL"
                     /locus_tag="J1138_004288"
     CDS             1455..2675
                     /gene="wcaL"
                     /locus_tag="J1138_004288"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBA8455998.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVIAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASVLQAL"
     gene            2686..4098
                     /gene="wcaM"
                     /locus_tag="J1138_004289"
     CDS             2686..4098
                     /gene="wcaM"
                     /locus_tag="J1138_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBA8455999.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARAIPAQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEESGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHLIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKRKTLLSLTNIHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRELICDHS"
     gene            4238..5233
                     /locus_tag="J1138_004290"
     CDS             4238..5233
                     /locus_tag="J1138_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA8456000.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALVGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            5476..6369
                     /gene="galF"
                     /locus_tag="J1138_004291"
     CDS             5476..6369
                     /gene="galF"
                     /locus_tag="J1138_004291"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBA8456001.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         1819 a         1618 c         1699 g         1624 t
ORIGIN      
        1 gcgtcagcgt cgcatcaggc actgaatgcc ggatgcggcg tgaacgcctt atccgtccta
       61 cggttctgtg ctcggatctt gtaggcctga taagacgcgt tagcgtcgca tcaggcaaaa
      121 aacggcaacg aacacaacaa acacagcctc acctggcatc aaatttatga ggaaaaaatg
      181 aaattactta ttctgggcaa ccacacttgc ggcaatcgtg gcgacagcgc catcctgcgt
      241 ggcttacttg atgccatcaa tatcctcaat ccacatgccg aagtggacgt gatgagccgc
      301 tatccggtca gttcttcctg gctgctcaac cgcccggtaa tgggcgatcc gctgttcctg
      361 caaatgaaac aacacaacag cgcggcgggc gttgtcgggc gcgttaaaaa agtcctccgt
      421 cgccgctatc agcaccaggt attgctctcg cgcgtcaccg acactggcaa gctgcgtaat
      481 atcgccatcg cccaaggatt caccgacttc gtgcgcttgc tgtcaggcta cgacgccatt
      541 attcaggtcg gcggatcgtt ttttgtcgat ctctatggcg tgccacagtt tgaacatgcg
      601 ctttgcacat ttatggcgaa aaagccgctg tttatgattg gtcacagcgt cggccccttc
      661 caggatgagc aatttaacca actggcgaac tatgtctttg gtcactgcga cgcgctgatc
      721 ctgcgcgaat cggtcagcct tgatttgatg aaacgcagca atatcaccac tgcaaaagtt
      781 gaacatggcg tcgataccgc gtggctggtc gatcaccaca ttgaagactt caccgccagc
      841 tatgctgttc aacactggct ggatgttgcc gcacaacaga aaacggtggc aattaccctg
      901 cgcgaactgg caccgttcga caaacgtctc ggcaccaccc aacaagcgta tgaaaaagcc
      961 tttgccgggg tggtcaatcg cattctcgat gaagggtatc aggtgattgc gctctccacc
     1021 tgtacgggca tcgacagcta taacaaagac gaccgcatgg tggcgctcaa tctgcgccag
     1081 cacatcagcg atcctgcccg ttaccacgtg gtgatggatg aactcaacga tctggaaatg
     1141 ggcaaaattc tgggggcctg tgaactcacc gtcggtacgc gcctgcactc tgccattatc
     1201 tcgatgaact ttgccactcc ggcgattgcc atcaactacg aacataaatc cgccgggatc
     1261 atgcaacaac tggggctacc agaaatggcg attgatatcc gtcatttatt agacggtagc
     1321 ctgcaagcga tggttgcgga taccttaggc cagcttccgg cgctgaacgc acgacttagc
     1381 gaagccgtca gtcgcgaacg tcagatggga atgcggatgg tgcaatctgt gcttgaacgc
     1441 attggggagg cgaaatgaag gtcggcttct ttttactgaa atttccgctg tcgtcggaaa
     1501 ccttcgttct taaccaaatc actgcgttta ttgatatggg ctttgaagtg gagattgtcg
     1561 cgctgcaaaa aggcgacaca caaaacaccc acgcggcatg gacgaaatac aaccttgctg
     1621 ccagaacccg ctggttacag gacgaaccta cgggcaaagt ggcgaaactg cgccaccgcg
     1681 ccagccagac cttacgcggc attcatcgta aaaatacctg gcaggcgctc aacctcaaac
     1741 gctatggtgc tgagtcgcgg aacctgattt tgtctgccat ttgcggccag gtcgcaacac
     1801 cattttatgc cgatgtcttt atcgctcatt ttggccctgc gggggtaatc gcagcaaaac
     1861 tacgggaact gggtatcatt cgcggcaaaa tcgctactat cttccacggc attgatatct
     1921 ccagtcggga agtgctcaac cactacactc ccgaatatca gcaactgttc cgccgtggcg
     1981 acctgatgct accgatcagc gatctgtggg ccggaaggct gcaaaaaatg ggctgcccga
     2041 gggaaaaaat tgccgtatcg cgcatgggcg tggacatgac gcgctttagc ccgcgtccgg
     2101 tgaaagcgcc tgcaacgccg ctggaaatca tttccgtcgc acgcttaacc gagaaaaaag
     2161 gcctgcatgt ggcgattgaa gcctgccgtc agttgaaaga gcagggcgtg gcatttcgct
     2221 atcgcatcct cggcattggc ccgtgggaac gacgcctgcg caccctcatc gaacaatatc
     2281 aactggaaga tgtggtagag atgccgggct ttaaaccgag ccacgaagtg aaagcgatgc
     2341 tagacgacgc ggatgttttc ctgctgcctt cggtaacggg tgcggacggc gatatggaag
     2401 gcattccggt ggcgctaatg gaagcgatgg cggtcggcat tccggtggtt tctactctgc
     2461 atagcggaat tcctgaactg gtggaggccg ataaatccgg ctggttggtg cctgagaacg
     2521 atgctcgcgc actggcgcaa cgactggcgg cgtttagcca actggacacc gacgaactgg
     2581 ctccggtcgt caaacgtgct cgcgaaaaag tcgaacacga ttttaaccag caggtgatca
     2641 atcgagaact cgccagcgtg ctgcaggctt tatagaggag aacgtatgcc atttaaaaaa
     2701 ctctcccgac gcacgttcct gacggcaagc tcggtgcttg ccttcctcca tacccctttc
     2761 gcccgtgcaa ttcccgctca acaaagcgtt aacattaacg actacaaccc tcacgactgg
     2821 atcgcctcat ttaaacaagc cttcagcgaa gggcaaacgg tcgtcgtgcc tgccggattg
     2881 gtttgtgaca atatcaacac cggcatcttc attcctcctg gcaaaacgtt acacatcctt
     2941 ggaagcctgc gcggcaacgg cagagggcga tttgtcttac aggacggcag ccaggtgaca
     3001 ggggaggaga gcggcagtat gcataacatc accctggatg tgcgcggctc tgactgcacc
     3061 atcaaagggc tggcgatgag cggctttggt ccggtaacgc agatttatat cggcggcaaa
     3121 aacaaacggg tcatgcacaa cctgaccatc gataacctga ccgttagcca cgctaattac
     3181 gccatcttac gccagggatt tcataaccag ataatcggtg ccaacatcac caactgtaag
     3241 ttcagcgact tacagggcga cgctattgaa tggaacgtgg cgattaacga cagtgatatt
     3301 ttgatatccg accatctcat tgagcgcatt aactgtacca acggcaaaat caactggggc
     3361 atcggcatag gccttgcggg aagtacctac gataataatt atccggaaaa ccaggcagtg
     3421 aaaaactttg tcgtggcgaa tatcacggga tcggattgtc ggcaactgat ccatgttgaa
     3481 aatggtaaac attttgttat ccgtaatatc aaagctcgta atatcacgcc ggatttcagt
     3541 aagaaagcag gtattgataa cgccacagtt gctatttatg gttgtgacaa ctttgtgatt
     3601 gataatattg aaatgattaa tagtgccggg atgttaatcg gctatggggt aattaaaggc
     3661 aaatatctct cgataccgca gaatttccga gtgaatgata ttcaactgga taatactcat
     3721 cttgcttata aattgcgcgg cattcaaatc tccgccggga atgctgtctc ctttgtggcg
     3781 ctgactaaca ttgagatgaa gcgtgcgtcg ctggagttac acaacaaacc gcaacatctt
     3841 tttatgcgca atatcaatgt gatgcaggaa tcctcagttg gacccgcatt gagcatgaac
     3901 tttgacatgc gcaaagacgt tcgaggcgtt tttatggcaa agcgtaaaac gctgctttca
     3961 ctcacgaata ttcatgcagt gaatgaaaaa gggcaaagct ccgtcgatat cgacaggatt
     4021 aatcaccata ttgttaatgt ggagaagatt aactttagat tgccggaacg gagggagttg
     4081 atttgcgacc attcataaac ttactggata taatgttcta aaagattata atcctcacac
     4141 taactgttaa agctgggact gggcagcatt gactactcag ggaaggtact catctttatc
     4201 ttgtgtttct tacgtaacaa ccagactaaa gtgatttatg aacgataacg ttttgctcat
     4261 aggagcttcc ggattcgtag gaacccgact acttgaaacg gcaattgctg actttaatat
     4321 caagaacctg gacaaacagc aaagccactt ttatccagaa atcacacaga ttggcgatgt
     4381 tcgcgatcaa caggcactcg accaggcgtt agtcggtttt gacactgttg tgctgctggc
     4441 agcggaacac cgcgatgacg tcagccctac ttctctctat tatgatgtca acgttcaggg
     4501 cactcgcaat gtgctggcgg ccatggaaaa aaatggcgtt aaaaatatta tctttaccag
     4561 ttcagttgct gtttatggtt tgaacaaaca caaccctgac gaaaaccatc cacacgatcc
     4621 tttcaaccac tacggcaaaa gtaagtggca ggcagaggaa gtactgcgtg aatggtataa
     4681 caaagcacca acggaacgtt cattaaccat catccgtcct accgttatct tcggtgaacg
     4741 caaccgcggt aacgtctata acttgctgaa acagatcgct ggcggcaagt ttatgatggt
     4801 gggcgcaggg actaactata agtctatggc ttatgttgga aacattgttg agtttatcaa
     4861 gtacaaactg aagaatgttg ctgcaggtta tgaggtttat aactacgttg ataagccaga
     4921 cctgaacatg aaccagttgg ttgctgaagt tgaacaaagc ctgaacaaaa agatcccttc
     4981 tatgcacttg ccttacccac taggaatgct gggtggatat tgctttgata tcctgagcaa
     5041 aattacgggc aaaaaatacg ctgtcagctc tgtgcgcgtg aaaaaattct gcgcaacaac
     5101 acagtttgac gcaacgaaag tgcattcttc aggttttgtg gcaccgtata cgctgtcgca
     5161 aggtctggat cgaactctgc agtatgaatt cgtccatgcc aaaaaagacg acataacgtt
     5221 tgtttctgag taatgcagac gagtcagaga aaaaagaacc aggttggact acgattattc
     5281 ggaaagaggt actgattaaa ttatcagtat gtggatttcc gagtaatccg agtaacccag
     5341 atgctgatgg tgtgtagtgt tatcgtaagg tttaatcaaa gttatgcgga gctaagcttt
     5401 ggtatatagc cataacgttt ggctaaccta tggcaataat ttacgcacta tcagccactt
     5461 agaggtagga aaagaatgac gaatttaaaa gcagttatac cggtagcagg tcttgggatg
     5521 catatgttgc ctgccactaa ggcgataccc aaagagatgc taccgatcgt cgacaagcca
     5581 atgattcagt acattgttga cgagattgtg gctgcaggga tcaaagaaat cctcctggtg
     5641 actcatgcgt ccaagaacgc ggtcgaaaac cacttcgaca cctcttatga attagaatct
     5701 ctccttgaac tgcgcgtgaa gcgtcaactg ctggcggaag tacagtccat ctgtccgccg
     5761 ggcgtgacca ttatgaacgt gcgtcagggc gaacctttag gtttaggcca ctctattttg
     5821 tgtgcacgac ccgccattgg tgacaatcca tttgtcgtgg tactgccgga cgttgttatc
     5881 gatgatgcca gcgccgaccc gctgcgttac aaccttgccg ctatgattgc gcgcttcaac
     5941 gaaacgggac gcagccaggt gctggcaaaa cgtatgccgg gtgacctctc cgaatactcc
     6001 gtcatccaga ccaaagagcc gctggaccgt gagggtaaag tcagccgcat tgttgaattt
     6061 atcgaaaaac cggatcagcc gcagacgctg gactcagaca tcatggccgt aggtcgctat
     6121 gtgctttctg ccgatatttg gccggaactg gaacgtactc agcctggtgc atggggacgt
     6181 attcagctga ctgatgctat tgccgagctg gcgaaaaaac aatccgttga tgcaatgctg
     6241 atgaccggcg acagttacga ctgcggcaaa aaaatgggct atatgcaggc gtttgtgaag
     6301 tatggcctac gcaacctgaa agaaggggcg aagttccgta aaggtattga gaagctgtta
     6361 agcgaataat gaaaatctga ccggatgtaa cggttgataa gaaaattata acggcagtga
     6421 agattcgcag caaaagtaat ttgttgcgaa tattcctgcc gttgttttat ataaaccatc
     6481 agaataacaa cgagttagca gtagggtttt agtcaaagtt ttccaggatt ttccttgttt
     6541 ccagagcgga ttggtaagac aattagtacc ttattattta tataagaaaa tgaatgggta
     6601 acaccaattg agtcatagac atgtctgcag tacgctggta gctgttaagc caggggcggt
     6661 agcattctta tctatatgga ataaaagatg aaagtattat ttttaattac aagaggagat
     6721 gagttaggtg gcgcacagac tcatgtcaaa gatgttatat
//
LOCUS       DADTFG010000201         6473 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.202, whole genome shotgun sequence.
ACCESSION   DADTFG010000201 DADTFG010000000
VERSION     DADTFG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.202"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..505
                     /locus_tag="J1138_004292"
     CDS             <1..505
                     /locus_tag="J1138_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA8456002.1"
                     /translation="VHPRLTALRVDLRLPDISAETDAAVISRFVNALKARIDAYQKRK
                     RREDKRVHPTTLHYAWAREFGERKGKKHYHLLLLVNRDTWCRAGDYRAPESLAGMIKQ
                     AWCSALGVDAGCHATLATFPEHPALWLTRDDDAGFQQLLERADYLAKESTKAYGTGER
                     NFGCSRG"
     gene            673..1055
                     /locus_tag="J1138_004293"
                     /pseudo
     CDS             673..1055
                     /locus_tag="J1138_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1052..1477
                     /locus_tag="J1138_004294"
                     /pseudo
     CDS             1052..1477
                     /locus_tag="J1138_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1678..1920
                     /locus_tag="J1138_004295"
     CDS             1678..1920
                     /locus_tag="J1138_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA8456003.1"
                     /translation="MVSINHTRESSPYLSVPQDDFCIVNLGGCIVHFPANGRDILDIN
                     DPDFPEQYQRLLARLDYLTKLDTKASGQRNSGYSGF"
     gene            1948..2370
                     /locus_tag="J1138_004296"
                     /pseudo
     CDS             1948..2370
                     /locus_tag="J1138_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(2379..3592)
                     /locus_tag="J1138_004297"
     CDS             complement(join(2379..3298,3298..3592))
                     /locus_tag="J1138_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA8456004.1"
                     /translation="MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPEQQRIRELEKQVRRLEEQNTIFKKG
                     YRALDVRLTERFTIVSRLSDSHSVVSLCSALGIHRSSYRYWRKRRDTVNSARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNACQE
                     HTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTPEESENR
                     YRFYCKTVANIT"
     gene            4096..5292
                     /locus_tag="J1138_004298"
     CDS             4096..5292
                     /locus_tag="J1138_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBA8456005.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRGEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            5363..5596
                     /locus_tag="J1138_004299"
                     /pseudo
     CDS             5363..5596
                     /locus_tag="J1138_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015841669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter solute-binding protein"
     gene            complement(6065..6286)
                     /locus_tag="J1138_004300"
     CDS             complement(6065..6286)
                     /locus_tag="J1138_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456006.1"
                     /translation="MKEGAGIKAGHRNANINADYRLLELTVINQHKPLTIKQILINKI
                     LANTITALTTLQFAFSISYRCQTILFYML"
BASE COUNT         1507 a         1665 c         1652 g         1649 t
ORIGIN      
        1 ggtgcatccc cgcctgacgg cacttcgggt ggatctgcgt ctgccggata tatccgcaga
       61 gactgatgct gctgtgatat cccgcttcgt caatgccctg aaagccagaa tcgacgctta
      121 ccagaagcgt aagcgccgtg aagataaacg cgtacatccc acgaccctgc attacgcctg
      181 ggcccgggaa ttcggggagc gtaaaggtaa aaaacactat catctgctgt tgctggtcaa
      241 ccgggatacc tggtgtcgtg ccggtgatta ccgcgctccg gagtctctgg ccgggatgat
      301 taaacaggca tggtgcagtg ccctgggagt ggatgccgga tgccatgcca cgctggcgac
      361 ctttccggaa catcccgcac tgtggctgac gcgtgatgat gacgccggct ttcagcagct
      421 actggaacgg gctgattatc tggcgaagga gagtaccaaa gcttacggca ccggtgagcg
      481 caactttggc tgcagtcgtg gctaagcccg acagacgact tatctgacct tagtgtgatg
      541 acgtgttcac agcgaccaca ctgattttcc cttctgagac tgaactgaaa accgccattc
      601 acacctgacc acgctgcccg taccgggacg actgttgcct gcgtgtcgtc tggcggctga
      661 ggaatatata ctatgtacgc agaatccttt ctcgcacttg atggtaacgg acgtctgacg
      721 gggcccgtac cgcacagact gctccttatg cccactacac ctgccacttg tgtggcagtg
      781 cactcagata ccacccgcaa tacgacaccg aacggccctg gtttgaacac actgacgacg
      841 ggctgacaga gcacggtcaa cagtgtcctt atgtcagacc ggagcgcagg gagatacaac
      901 tgattaaacg tctgcagcaa ttcgtaccgg atgccttacc cgtagtacgt aaagccagct
      961 ggcactgcag gcaatgtcac cacgattatt atggggagcg gtactgcaca cactgccaga
     1021 ccgggcgctt cagtgaggag gggggagcag aatgaacggt ataaaaacgg ggctggggat
     1081 cacgccgggt gagcacatta tcagtgccga ctctgcgctc agcagaaata tccggcagtg
     1141 tttctgcctt tcctgtcatg gccggttgat tctgcagaca gatgctcagg gggcgtggtt
     1201 tgagcacgat ttacatgccc tgagctagca acagaaggcg gactgtgtgg tgctgaatcc
     1261 ggagaagtcc cacccttaca ttgaggatat ggcgatgttt ttatcgccat taccggtcgt
     1321 gctggagtgg cattgtgtga tgtgtgagca gttttttcat gggaagaagt tctgtgaggc
     1381 ctgtggtacg gggatttatt gcagagcggt atgtacgaag tctgtttaca gctaccagcc
     1441 agacttgttt gaggactgcg gaggtgcgag tacttaatcc gaaatctccg ctctgcgcag
     1501 ggcgttcaga ggtatcgcaa cctgcatttt atccggtgtt tcgcacctgc cccgttcgtg
     1561 acacaaaggc gcgacaccgg atatccgcag tcctgcttac ctgaataaaa cccggggtat
     1621 tgattggtag tgtggtgcgg aatcgtaccg tgatctcgca ttcgccgaat tcgcactgtg
     1681 gtgtcaatca accatacgcg agagtcctct ccgtatttat cagtgcctca ggacgatttc
     1741 tgcatagtta atctaggggg ctgcattgtt catttccctg ctaatggacg tgacatcctg
     1801 gatattaacg atcctgattt ccctgagcag tatcagcgtt tattggcacg tctggattat
     1861 ctgacgaagc tcgacactaa agcatcaggc cagcgtaact ctggctacag cggtttttga
     1921 cctgttgtaa ctgtaaggtg atttattatg tatacagaat caaatgttaa gctttcctgt
     1981 aacgccctct ggcaaaagtg tgtccttgca actctgtatc gggctgcagc tacacatcct
     2041 gaaattaaat tattacaatt taaattaata ccagggcagg gtattaataa ggccattccc
     2101 tctctgatgg ctgattttac tggtgaactg catagactca tttctgaata tttgcagtgc
     2161 aagagacgta aagataaatc tgttgagccc acccctttat attatctctg gagtagggaa
     2221 gctgtggcta acgataccac tccttatctt atggtgctgc tacttaataa cgatgtctgg
     2281 tgtaagagtc gtgaggtggt ggttctcaga aatctgattg agcaggcgtg gggaaaagta
     2341 ttgggtgaag ataactccat tacacatctg tgtagtggtc aagtaatatt ggccacggtt
     2401 ttacagtaaa aacggtatct gttctctgat tcttccggcg tcaacccacc gttgtaatgg
     2461 tgaggcctga cgctattgta ataattcagg atataaccgc taatttgttg ccgggcctcg
     2521 tccttgccta cgtaaccatc cgtcggcacc cattctgttt tcagactgcg gaagaagcgt
     2581 tccataggac tgttatccca gcagtttccc cgtcggctga cactttgctt tatcctgtaa
     2641 cgccagagaa gctgttgata tttcagtcct gtatactgac ttccctggtc gctatggaac
     2701 atgacgtccc gcggctgacc acgcacctca tacgccatcc gcaaggcact gcttatcagt
     2761 gcagtatcgg cattcgctga caggctccag ccgataaccc tgcgggcaaa aagatccatg
     2821 acgaccgcca gatagcacca gcgatttcct gcccagatat acgtaatatc tccgcaccat
     2881 accctgtctg gctccggtac agcgaactga cgctcgagca gattcggcaa acacgtatgc
     2941 tcctgacagg catttttgta ttgatgtttt ccgggctgac aactgctcag gttcaggtat
     3001 ttcatcagcc gtccggcacg ataacggctc atcgggacgc cgttttgggt cagcatttca
     3061 gccagcgtgc gcgcgcctgc agagccccgg ctctggttcc atgcccggcg tatttcgctg
     3121 cacaacctga cgcgagccga attaaccgta tcgcgtcgtt ttcgccagta ccggtaactg
     3181 ctacggtgta ttcccagtgc ggaacagagg ctgacaactg agtggctgtc actcagtctg
     3241 gaaactatcg taaaccgttc agtgagtcgg acatcaagag cgcggtagcc ttttttaata
     3301 tcgtattttg ttcctccagt cggcgaacct gcttttccag ctcgcgaata cgttgctgtt
     3361 caggggtaat gggtgttgca gagggcgcaa taccttggcg ctctcgcctg agctgacgta
     3421 cccagctctc aagcgtggta gaaccgacat tcatcgcctc actggcttgt cgatacgaat
     3481 agcccttatc aacaatcagc tgtgcacatt ccagcctgaa ctcaggggtg aaagtacgtt
     3541 tggttttctt gttcattaag tcacctgttt tgtgttgagg tgagaatatc acctttaatc
     3601 aggtggccaa atttactgtg ccactacatc tggttgtgta tcctgaagtg ttgtcgttgg
     3661 tatttaatgc agctaacaca tacaacgctg agtctggtgt actggaacag acggcccggg
     3721 cttacattga atcgcatggt gccattgaac gtcatcttgg gtgtagtgac ttttaacgtc
     3781 aggtgatgca taagggtctt tcggggagag gccctgttta cacaattaaa taagtcgagt
     3841 cctccaggct gggctgtgtg cacgccccgt tactttattc gtacccctta taatggggtg
     3901 ttagccagcc agacccggca tgattactgc ccccagtcgt ccatgatccg gggggtgatg
     3961 tcaccgggtc tggtggggcg ctggtaaccg ctaatagggg tcaggtcagg cacttttgcc
     4021 gggaccgtct gtaacgtgga tgccggtacc tgctccccgt ggttatctgg ttaacccata
     4081 tacaagggag acagaatgac cgaatccagc gattacgaat ccgtccaggt ctttatcggc
     4141 gttgatgtcg gtaaagatac gcatcacgct gtagccatta atcgttcagg taaacgcctg
     4201 ttcgataaag cattacccaa cgacgaaaac aaactcaggt cgctaatatc tgacctgaaa
     4261 caacatggtc agatactgct ggttgttgat cagccagcta ccatcggtgc gttacctgtc
     4321 gccgttgccc gctcagaagg agtccttgtc ggatacctcc ctggactggc catgcgccgc
     4381 atagccgact tacacgccgg tgaagctaaa actgatgctc gtgacgctgc catcattgcc
     4441 gaagctgccc gtaccctgcc tcacgcgcta cgcacgctga aactggctga cgagcaaatc
     4501 gccgaactct ccatgctctg cggcttcgat gatgatcttg ccgcacagac aacgcaggcc
     4561 agcaaccgta tccgcggcct tctgacccag atacatccgg cactggagcg cgttctcggt
     4621 ccgagacttg atcacccggc ggtactcgat cttctccagc gatatccctc accagaaaaa
     4681 ctcgcttcgc tgggtgagaa gaagctggca gcccagctct gcaaacttgc gcctcgtctg
     4741 ggtaaacgcc ttgcagcaga catagctcag gcactggccg aacaaaccgt cgtcgttccc
     4801 ggcacgaatg ccgctgccgt agtactgcca cgtctggcac tccagctcat cacgctgcgt
     4861 aagcaaagag gcgaggtagc gcttgaggta gaacagcgag ttcttgctca ccctctttac
     4921 ccggtcctga ccagtatgcc cggagtcggt gtcaggaccg cagccagact cctcaccgag
     4981 gtcgcctgcc gcgccttcgc ctctgccgca catctcgctg cttatgctgg ccttgcgccg
     5041 gtaactcggc gatccggctc gtcaatacgc ggtgagcatc cctcgcgacg gggtaataaa
     5101 gctctcaaac gggcgttgtt cctgtcggcc ttcgccgcgc tcagggatcc gctctccagg
     5161 gcttactaca cccgcaaaat gagtcaggga aaacgacaca atcaggcgct tatcgccctg
     5221 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
     5281 caggcgtcat aacatgcttg acaacttaat aggggcacgt taccaatact ggcttaatgg
     5341 ctacatacgg cggtcagttt acgcttacaa aaatgtggcg aaactgatta atcaaattaa
     5401 gagagagcac gtaaaattgt actttatcga aaaccagacc gatccccgtt tggtgaaaca
     5461 aatcgccaat gccagtggtg cacaagcggg aggagagctt tatccggaag cacttaccga
     5521 cagtagcggc ctcgctgcta cctacacggc agcgttcaaa cacaatgtcg ataccctcgc
     5581 tgctggcatg aagtaagaga gtaggcaaaa aagtaagtag atatcaggtt gccccatatc
     5641 atcaggtaac ctgtgacact tctcttgcca gagcgccatg ttccagcgcc tcactcacta
     5701 tctgcttatc atccgacttt ccctcatacc tgtctcgatt ataaagataa aacgatgatg
     5761 cccgggttca ttgtgcgaag gcatggcata tttgttcccg gtatcgtcag acagcatcat
     5821 tcgactctcc atctgctgtc tggccagaca aaagatggcc ttgtttgccg cggtggaaat
     5881 ggagggagga cgagcccaca agcatgaaaa acttaaccca ggctgtggaa gtgcaacttt
     5941 tgctccggtt gctgcagtaa aacacgagta tacgtctgcc gtacacattc ataagcgatt
     6001 ccgatggctc acttacgtgg agaaatggag tgccgtctgt tctttattta ttgtttgcgt
     6061 tatgttataa catataaaag agtattgttt ggcatctgta acttattgag aaggcaaact
     6121 gcagagtggt taatgcagta attgtattag ctaaaatttt gttaattaaa atttgtttga
     6181 ttgttaatgg tttgtgctgg ttgatgactg ttaattccag aaggcggtag tctgcattaa
     6241 tattagcatt acggtgacca gcttttattc cggccccttc tttcatgaaa caacgtattc
     6301 cgaagaataa gtttgagaat ggcagacgaa cttttattat tctgctgaat acagaagata
     6361 tgaattgttc ttgtttatgt cagatattca taacacaggt attatggtta actcataaca
     6421 ttaattcctg tatttttctg ctcaatggca gcgtcgtatg catatgtttt tat
//
LOCUS       DADTFG010000202         6398 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.203, whole genome shotgun sequence.
ACCESSION   DADTFG010000202 DADTFG010000000
VERSION     DADTFG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.203"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..415)
                     /locus_tag="J1138_004301"
     CDS             complement(<1..415)
                     /locus_tag="J1138_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBA8456007.1"
                     /translation="MSNIIYLSIKGKIQGLISEGCGSYASIGNKYQINHVDEIFVLQF
                     DHSLSREYNVVHHPIKFYKPIDKSSPLLNAALSENEELSAVFNFYRVNSGGCIERFYT
                     IELQGAHLSNILTCYPHSITHAGNQPEEVIVLNYKN"
     gene            665..1246
                     /gene="mdaB"
                     /locus_tag="J1138_004302"
     CDS             665..1246
                     /gene="mdaB"
                     /locus_tag="J1138_004302"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBA8456008.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            1277..1591
                     /locus_tag="J1138_004303"
     CDS             1277..1591
                     /locus_tag="J1138_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBA8456009.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1636..2523)
                     /locus_tag="J1138_004304"
     CDS             complement(1636..2523)
                     /locus_tag="J1138_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA8456010.1"
                     /translation="MIQKDKARVDIFGERFRTRASQLTPGLRAVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2520..3467)
                     /locus_tag="J1138_004305"
     CDS             complement(2520..3467)
                     /locus_tag="J1138_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA8456011.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3478..4515)
                     /locus_tag="J1138_004306"
     CDS             complement(3478..4515)
                     /locus_tag="J1138_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA8456012.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4524..5507)
                     /locus_tag="J1138_004307"
     CDS             complement(4524..5507)
                     /locus_tag="J1138_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA8456013.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTSMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5504..6313)
                     /locus_tag="J1138_004308"
     CDS             complement(5504..6313)
                     /locus_tag="J1138_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8456014.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1825 a         1699 c         1444 g         1430 t
ORIGIN      
        1 tatttttata attaagaaca atgacctctt ccggctgatt acccgcatgt gtaattgagt
       61 gtggataaca tgtcagaata ttcgataaat gtgctccttg caattcaatt gtgtaaaacc
      121 tttcaataca accgccagaa ttaactctat aaaaattaaa tacagctgat aactcttcat
      181 tttcagaaag agcggcattt aataaagggg aggatttatc tattggcttg taaaatttta
      241 tggggtgatg cactacatta tactctcggc ttaaagaatg gtcgaactgt aaaacaaaaa
      301 tttcatccac atgatttatt tgatatttat taccaataga tgcatacgaa ccacaaccct
      361 cagagatcaa accttgtatt ttccctttaa ttgataagta tatgatattt gacatatata
      421 ttctccataa tcattttttc cgaaaaactc ctgtatcatt tgcataatac atctctcctg
      481 tgggagctta tgggaagttg atttaagtat tgtcattttc agaaaatatt tatcccgcat
      541 cggtgaatca gagaaagatc agacttttgc taaactcgca aaagactttg cacattttgc
      601 taatttcacc gtaccgctct gtgacgtact atagtcggca aacgtctcac cttgaggtta
      661 aaaaatgagc aatatcctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
      721 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
      781 ccgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg
      841 ggctgatgtg gtgatctggc agatgcctgg ctggtggatg ggcgcgccgt ggacagtgaa
      901 aaaatatatt gatgatgtat tcaccgaagg tcacgggact ctgtatgcca gcgatggtcg
      961 tacccgcaaa gatccatcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata
     1021 tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcagttctt
     1081 ccacggcgtt ggtgttgacg gtgtgtacct gccgtttcat aaagcaaacc aattccttgg
     1141 catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
     1201 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctctggct
     1261 ttagaaggag ttaaccatga ttacagtaat cgcagaaatc cgtactcgtc ctggtcaaca
     1321 tcaccgtcag gcagtgttgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
     1381 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
     1441 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcatc ttgaagcgca
     1501 tctgcaaacc ccgcacatga aagcatatag cgaagccgta aaaggtgacg tgctggagat
     1561 gaatattcgt atcctgcagc cggggctttg acatttctaa aaagaggccg gtgctgctac
     1621 cggtctcagc ccagattagc cattctttct tcccgttttc agtcctcgat ggaaatcgtt
     1681 aattcgctta atggttttaa ccgcacgctg cggcactgcg gaagctaccg accagacaat
     1741 catttccaga cacaacaata ccgtaccatg tagcggcgtt ttcccttttt catccccgcg
     1801 cggaacatga atcacgatac tggcgtcttt gctaaaacgt gaatccagcg cgtttgtcag
     1861 caaaataaca gggatcccta gccgcctcgc ctcacgaagc gttgtcagcc cttcccggtg
     1921 cgctgatttt tgcgccatca tgatcagaac atcgccacgt tgaagcgcga tcagttgctc
     1981 ggcaaggcca atacccgtac ggtttagtgc cgtggcaggc aaacctatgc ggttgaatag
     2041 tcgtgcggta tagtccgcca gaatacccga agcgccaatg ccaaaaattg cgacttgccg
     2101 tgcctgcacc aacaacgcta cagcttgcgc aagcgcgtgg cggttttcag gctctgacag
     2161 gacattacag gtatacaaat gcccttcaag aacaaagtcg attgcggtgt taacatcgct
     2221 cgtcaagtta ctcaccgtcg tagacatctt ttcgctggaa ctaagggctg gaccaagcca
     2281 ctgttccaga gtgcgtttca gatcacgcag cccggcaaac cccagggcct gtatagccct
     2341 gataactgtg gcatcggaag tgttcaacgt ggccgcaatt tccatcgccg tttgctcgag
     2401 taccacttca cgatgctcat taatgtagct ggcgacagct cttaacccag gcgttaactg
     2461 actcgcgcga gtgcgaaaac gctcaccaaa aatatcaacc cgcgctttat ctttctggat
     2521 catttagcct ccggtagagg tcgccctgca atttgtgatt gtatctgcgc agccatcagc
     2581 cccagagatg caaaggagtt agaaattgcc tcttcacgag aaatcagcac ataacgcccg
     2641 gaacgacagg cagtcagaaa ctgacaccat cccggcatga ctttttccat tgctgccagc
     2701 tcatcctgag gtttaccacc ggtgtcaccg cgccaggttg caaaaacaaa atcggcatcc
     2761 agttcaggta aacgctccgc gctcacatcc attcgcccac cttcaggaat gctctcaatc
     2821 agcggcggaa aacgaaaacc cgcatcgcgt aatacccgcc ccagcgaatg atagctatgc
     2881 atcacattaa tttttccctg gtttgcctga atcaccgata ccgttatttt ttggctgtcc
     2941 agcgtcgcct tcagcgcatt gatttgtgcc tgatagcggc gttcaagtat cgccagctgc
     3001 gactgcgttc ctgtaagttc tgccagcttg cgatagattt ctggcgcacc ccctttgaga
     3061 tgatcaatgc tcaccgtggg cgcaattttt tcaagttgtt caatgggcgt attacgggtc
     3121 ggttctgtga tgatcaggtc aggctttgcc gccacaatcg cttcaatatc aatatcagcg
     3181 gtaccaataa aggcgatcga tgaattatca aagtcgacac ctgtcaataa cgcgccagat
     3241 cgaatgaaat ggctaccgtc aggccgagtt cgcccgtggc tggcgacagg aggtacaccg
     3301 agctcaatca gaggaatagt gatatcaaga tcgtgtaaag agacaattct ctttggatga
     3361 gcaggaacag tgactttgcg ccctaaatca tcagtaaata cctgaacagg ttctgttgca
     3421 cgggaaaaaa atgacaacag tatcagcaga gaaaaaaata aacgcatata actcccgtta
     3481 aaaactgttc ctgcgctgcc agagcagtag caaaaaaaat ggtccaccaa tcagtgagat
     3541 aatgattccg gcaggcagtt gcaaaggcaa aaaagccaaa cgaccaatgc tatccgccag
     3601 aataactaac aacgcccccg ataccgcgct ccccagcagt aatgtcgtct gcccgccacg
     3661 tagaataaaa cgtgatatat ggggtgctat caatccgaca aaaccgatgt taccaacaca
     3721 cgaaacgcag acggctgtca ggattatggg tgcggcgaca cgtaacaacg ccagacgaga
     3781 actattcacc cctaacccgg ttgcaacctg atgaccaagc agagcgatat ccgcagtcct
     3841 ggcggtgaac agcaataaaa gagcggctgg caacatccaa caggctgaaa taccgactaa
     3901 catccagtta gctgcatgca ggcttcccga taaccacatc aatgcagttt gtacatctcg
     3961 aacatctgca gtggtcataa atacaccgat accggcggcg aaaaaccagg aaacaccaat
     4021 accaatcaga acaaatcgcg ggcgggaaat atcgcgcgca caaaaaatca cgattaaagc
     4081 caccaacaga ccaccagcaa gaccagccac tggacgccag aacattccca gcattggaaa
     4141 ctggaaaatc agccacagca ccgccacgct acatccttct ttgacgccga ttaaaccagg
     4201 atcggcaagg ccgttacgcg caatggattg catcgcggcc cccgccatac ctaacatggc
     4261 cccgcatagc aatgccatga tgactctcgg caagcgaata tcctgcacaa tataacgtgc
     4321 gtctgtgctt acgttttctg gagcaaacaa cgctcgtcct acttccgatg ccggaacaga
     4381 gaaagaaccc tgcatcagac cgaatatcag tattccaacc gcaagcagga acagcccacc
     4441 aagcaacagc agatttttag ggcgcaccag tgtcgaaaac ttaccaacac gtagtgggcg
     4501 tagcccgacg ttcattatcc gactcatttg aacatcctcg tcgccataat gacaaaaaca
     4561 ggcgcaccaa ctaacgccgt catgacaccg gtcgctaact catggggcgt gaaaagcgtc
     4621 cgggcaataa tatctgccag taataacaaa agcgcgccga cacaagctga aagagggagc
     4681 agaacgcgca gatccgctga taccagacgc cggacaatct gcggtactag caggccgaca
     4741 aaaccaatgg ggccagcaat cgatacggct gcgccacaga gcaacgcgat cgcaagcagg
     4801 gtgaatgttc gcgtgcggag tacagagacg cccagcccct gcgccatgcg atcgcccagt
     4861 gccaacatgt taagggaggg tgcaaggtaa agggctaaca caaatccccc cagagaaaac
     4921 caggcggacg tacccagcgt cgcccaatcc tgccccgcta aatcacctgc tagccatgtc
     4981 cgcatcgcca gcagtgtttg ttcgtcaaga atcaggattg cagccgttat tgaggacacg
     5041 aacgccgata gcgccacgcc gcataaagta actttcattg acgttaaccc tgaacgccct
     5101 gcggaggaaa gcagcaatat caacaggaac aataacgcgc cccccgtaga tgccagcagt
     5161 gggcgtccaa ccggaaaggc cagtccaagc gcactagcgg caacgacagc aagagcggct
     5221 ccggcattta agcctaaaat atgtggctcc cccaatgggt tacgaatgac agcctgcaac
     5281 aaagcaccag caacaccaag agaagcaccg gtaagcaatg ctgccgctaa acgcggcaaa
     5341 cggagtctta caattacgtt gtgatcgaag ttttgcggat caaaatgaag aaatgcctgc
     5401 aacaccgttt gtggtgcaat aaatcgggca ccaatccctg gatataccac gcatcccacc
     5461 agcagtaagg cgaggaaaat tacagtaacc agccctgtgc gcatcatact ttttctacac
     5521 cacgaaacgg catgaagaac ggctttcccg tcagaggatt tattgatgcg tgaacatcaa
     5581 cgtcaaagac cgcttttacc agctcaggcg tgcaatgctc cccctcattc aacacacgca
     5641 ccactttccc ctggcgtaaa aatatcaacg tatcgccata attgacggca aaattcagat
     5701 catgcaacac cacgacgact gtacgcccgt gatgacgcgt taaatcatgt aataactcaa
     5761 gaatttcaac ctgataacgt aaatcaagcc aggttgtggg ttcatccagc aggatataag
     5821 gcgtcttttg cgccaacacc atcgcaatcc agcaacgctg gcgctgccca ccagagagtt
     5881 tttcaacagg taaatgtgca aactcctgcg taccggttaa tttcagggcc tcttccaccg
     5941 cctgctcgtc ggcgtcgctc cattgacgta tgaaattttg ccagggaaaa cgcccacgcg
     6001 acaccagttc ataaacggtt aagccctccg gcaataatgg cgactgcggc aagatcccaa
     6061 gtctacggga taacgctttc gttggttgct cgtgaatcgc tttgccatca agcaatatcg
     6121 aaccgcccat aggttgcaag atacgtgcaa tggtgctgag caacgtcgac tttccacagc
     6181 cgtttgcccc aaccagaacg gtcatttttt ctgtgggaac ggagaaagaa acaccatcaa
     6241 caatgatctt cttgtgatac ccggcagaaa cattgtcgag gatcagcccc tgcccttttg
     6301 cgttatgtgc cacgtgtacg ccaacttaat caaagacaaa caaaaataaa actcattctc
     6361 ctttgagagt ttgacgccac aaaatgtagt agtcaagc
//
LOCUS       DADTFG010000203         6330 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.204, whole genome shotgun sequence.
ACCESSION   DADTFG010000203 DADTFG010000000
VERSION     DADTFG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.204"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..755)
                     /locus_tag="J1138_004309"
     CDS             complement(<1..755)
                     /locus_tag="J1138_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBA8456015.1"
                     /translation="MQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLS
                     RVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFFRALQGVAGAFMIPLVFTTAFIYY
                     QGKELGLAAAVVSALASLSPTLGPTLGGWITDNLDWRWLFYINILPGIYLVLSIPFLV
                     NFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSFI
                     LFAARTLKISNPIMDLHAFKDKNFTLGCFFSFSG"
     gene            complement(731..1645)
                     /locus_tag="J1138_004310"
     CDS             complement(731..1645)
                     /locus_tag="J1138_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA8456016.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIFKLYRHP"
     gene            1742..2557
                     /locus_tag="J1138_004311"
     CDS             1742..2557
                     /locus_tag="J1138_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA8456017.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRIWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            2907..3059
                     /locus_tag="J1138_004312"
                     /pseudo
     CDS             2907..3059
                     /locus_tag="J1138_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3070..3585
                     /gene="ogt"
                     /locus_tag="J1138_004313"
     CDS             3070..3585
                     /gene="ogt"
                     /locus_tag="J1138_004313"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBA8456018.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKESYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTQFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            3780..4532
                     /locus_tag="J1138_004314"
     CDS             3780..4532
                     /locus_tag="J1138_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HBA8456019.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            4684..5634
                     /gene="uspE"
                     /locus_tag="J1138_004315"
     CDS             4684..5634
                     /gene="uspE"
                     /locus_tag="J1138_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBA8456020.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5684..5941)
                     /locus_tag="J1138_004316"
     CDS             complement(5684..5941)
                     /locus_tag="J1138_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBA8456021.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            6185..>6330
                     /locus_tag="J1138_004317"
     CDS             6185..>6330
                     /locus_tag="J1138_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HBA8456022.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFL"
BASE COUNT         1752 a         1490 c         1413 g         1675 t
ORIGIN      
        1 ccagaaaaag aaaagaaaca tcccagtgtg aaatttttat ccttaaaagc atgaagatcc
       61 attatcggat tggatatttt aagtgtacgc gcagcaaata aaataaaact caccaatgcc
      121 agaactgatg tcagcaaaat agtattatca tccagccatc cccagcgcgc tccttcttca
      181 agggtatatt ccagacatcc cagtgtcatg gccaacagga taatactcgg ataatcagca
      241 actttcagta aagagagatc aggtttatca aaattaacca gaaaaggaat actcaaaact
      301 aaatatatgc ccggtaatat attgatataa aatagccatc gccagtcgag gttatcagtg
      361 atccagcccc ccagagttgg ccctagcgtc ggtgacagtg aggccagcgc actgactacc
      421 gcagcagcca accccaactc tttaccctgg taataaataa acgccgtcgt gaaaactaaa
      481 gggatcatga atgcgccagc aactccctgt aaagcacgaa aaaaaatcat gatctgaata
      541 ttccatgcaa gtccacaagc gatactcatt aaagtaaata ttcccgcgga tagagtaaaa
      601 agccaacgag tggaaaatac ccgtgacagc cacccagaga gcggaataac aataatctcg
      661 gcaattaaat agctggtctg aagccaggcc atttcgtctt tacctgcgga cagaccacca
      721 cctatttcat ttagggatga cgatacaatt tgaatatcaa gctgaaactg gctcctgtcg
      781 ctggggaaag gctttcaatg tgaccgtgaa acacttcccc tttgagaata tcagcacgga
      841 tctcagcctt cattcctggc ttcattcccg ctatttggtt ttctttataa tttgcatcaa
      901 cccacaactc actgactggt actagtgaaa ctaacgatgt gccgccttcc acccaactgc
      961 ctgtatgggc gctgcggttt gcgacaatgc catcaatcgg cgcacgaaca agggtatatt
     1021 ccagatccag tagtgcctga gtcaaagcgg cctgaacttc ttcgatactt gcacgtacgt
     1081 tttcttcctg agcagataaa actgccagcc gttgcttctc caccaggaaa tcgttcgcgg
     1141 ctttgcgctc ctttgctgcg acctgctggt agtcaaacct ggcgttatca ataatttgtt
     1201 gcgatattgc cgctgattgt gcgagttttt cataacgttc agtatctcgc aggcgctttt
     1261 gctcctcatg ttttactgcc tgccatgttt cactggcact ttgaattatc gactgctgca
     1321 tcgcaatagt ggcttgaatg ctttcgagat tggctttgct actttttatt tttgcctcaa
     1381 gcctggcgac atttgcacga taatcccgat catcgagacg caggataata tcgccttttt
     1441 ttacactctg attatcgcgc acctctatag cagaaatata accagaaact ttacttgcca
     1501 ccgtggtgat attaccacca atgtaagcat catccgtcgt ctgtataaat cttcctttgc
     1561 ttacccacca gataaaaaag acaacgccag ccaaaagaat accgattgct accaccccaa
     1621 taagctgttt tttagaaatt atcatcactc tttacaccgt atatatccgg aaacttgcat
     1681 acgtcatatt taaaatttaa catggcttat ttgatgattt ctgacaaaat acctactgta
     1741 aatgacaatc acactgcaac ccaaagcatc accagggcat cacattatcg gactggattc
     1801 ggaacatctg aatggaggca cagttccctg gcataaacat ctctatgccc agttgcttta
     1861 cccggctgaa ggagtcgttc gtatatgggc tggagaaagc gtctggctag tgcatgccag
     1921 cagcgcgctt tggctgcccc cacaaatgcc gcataagttt gttgcgacag gcaacgtact
     1981 tttaaaaacc gtactggttt cagaagcgga aagtgaaacg ctgggaaaag tttgcttcat
     2041 gacgggtatt tcacccctgt taagggaatt actaattgca attaaccaac ttccaccgtc
     2101 gcaaagtacc acagataaac aacagttacg tttttcagcc ctcgagacac tgatacttca
     2161 ggaaataaaa atgggggtga aaatgtcact tgaactccct tggccaaatg atgagcggct
     2221 acagcagctt tgtgagaatt tgctaaacaa ccaagggtat ctgccaacac tggataatct
     2281 agcggataaa attaacgtca gtagccgtac actcatgcgc ttatttgtta aagagacggg
     2341 attaacattt cgccactggg ttcagcaaat gcacgttatt tctgcagtga cgcttcttga
     2401 tgatggatat tcgttgacca aaattgccca tcgtctgggc tatgcctcag ctgaatcgtt
     2461 cggcaatatg tttaagcgtc gaacgggcta ctcacccggt aaatttactc gtcgattaac
     2521 gatgcatgat tatgcaatca caagacaaat gatttaactt atattttcgt gaaatctgtc
     2581 actgaagaaa attggcaacg gatgggtaaa accgttataa cactgtcacc ggtcaccagg
     2641 accccaggcc ggatttaaga cgaggatgca ctgctgtgtg tactgtagag tctggcggat
     2701 gtcgacaggc tctatttttt tattcatttt ttagctctgt aaataaccac attctcgccg
     2761 ggattgggga atgagtgacg agatcagtgg tgtctggcgg ctatcacgcc acagaatcaa
     2821 ataacggata gcctacggta aaatttcgct gactggaatt tgatggcttg ccctcatttc
     2881 ttcaggcaga aaaatcaaaa aatcgcttta tgcttaaata cttcgacaaa ccagacgcta
     2941 aacctggtac ttactttttg ttagtattaa tcttttcggt ggtatggctg ctgatgttgc
     3001 tggcgtggta tcttgttggc ttgccgatag gtccgggtat ttacccacgt ttgtcttaag
     3061 agagaacgga tgctgagatt acttgaagaa aaaattgcca cgccactggg tccactgtgg
     3121 gtgatttgcg atgagcaatt tcgcctgcgg gcggttgaat gggaagagta cagcgaacgc
     3181 atggtgcagc tgctggacat ccattatcgc aaagaaagct atgagcgcat ttctgccacc
     3241 aacccaggtg gtttaagcga caagcttcgt gaatattttg ccggtaatct tagcattatt
     3301 gatacgcttc ccactgccac gggggggacg caatttcagc gcgaagtctg gaaaacactg
     3361 cgcactatcc cctgcgggca ggtaatgcat tatggccaac tggctgaaca attaggccgc
     3421 cctggcgcgg cgcgtgccgt tggtgcggca aacggatcga atcccatcag catcgtcgta
     3481 ccttgccatc gggttattgg ccgaaacggc accatgaccg gatatgcagg cggagttcag
     3541 cgaaaagagt ggttattgcg ccacgaaggt tatcttttgc tgtaaacatt aaacaatttg
     3601 tgccagcttg ttcacacttt tatgtaaagt cacccttaac aacttaaggg ttttcaaata
     3661 gatagacata tatttacatc taatatcgga attctctgct gttaaggttt gcttagactt
     3721 acttgctccc taaaaagatg ttaaaattga caaatatcaa ttacggcttg agcagaccta
     3781 tgatcccgga aaagcgaatt atacggcgca ttcagtctgg cggttgtgct atccattgcc
     3841 aggattgcag catcagccag ctttgcatcc cgttcacact caacgaacat gagcttgatc
     3901 agcttgataa tatcattgag cggaagaagc ctattcagaa aggccagacg ctgtttaagg
     3961 ctggagatga acttaaatcg ctttatgcca tccgctccgg tacgattaaa agttatacca
     4021 tcactgagca aggcgacgag caaatcactg gtttccattt agcaggcgat ctggtgggat
     4081 ttgatgccat cggcagcggt catcacccga gtttcgcgca ggcgctggaa acctcgatgg
     4141 tatgtgaaat cccgttcgaa acgctggacg atttgtctgg taaaatgccg aatctgcgtc
     4201 agcagatgat gcgtctgatg agcggtgaaa tcaaaggcga tcaggacatg atcctgctgt
     4261 tgtcgaagaa aaatgccgag gaacgtctgg ctgcattcat ctacaacctg tcccgtcgtt
     4321 ttgcccaacg cggcttctcc cctcgtgaat tccgcctgac gatgactcgt ggtgatatcg
     4381 gtaactatct gggcctgacg gttgaaacca tcagccgtct gctgggtcgc ttccagaaaa
     4441 gcggtatgct ggcagtcaaa ggtaaataca tcactatcga aaataacgat gcgctggccc
     4501 agcttgctgg tcacacgcgt aacgttgcct gattcttctg cataactcac tatccttctg
     4561 tcatatcatt aaatttttct gatttattga tctgacagaa ggttcatcac tgtttcattc
     4621 accagatatg ggttaatctt ttaattacaa actgcgttga cagttgttgt aaggagaccc
     4681 tgtatggcta tgtatcagaa catgctcgtt gttatcgatc ctaaccagga cgaccaacca
     4741 gcattgcggc gagctgttta tttacatcaa cggattggtg gcaaaattaa agcctttttg
     4801 ccgatctatg acttctcata cgaaatgacc accctgctct ctccggacga acgtaccgct
     4861 atgcgtcagg gcgtcatcag ccagcgtaca gcctggatcc acgagcaggc aaaatattat
     4921 ctcaatgctg gcgttcccat tgaaattaaa gtggtctggc ataaccgtcc tttcgaagcc
     4981 atcattcagg aagtgatcag cggcgggcac gatttggtgc taaaaatggc gcaccaacat
     5041 gaccgtctgg aagcggtgat ttttacgcca acggactggc atctgttacg caaatgccca
     5101 agcccggtgt ggatggtgaa agaccagccg tggccggaag ggggtaaggc gctggtggcg
     5161 gtgaatctcg ccagtgaaga gccgtaccat aatgcgctca atgaaaaact ggtcaaagag
     5221 acgatcgaac tggcagaaca agtcaaccat accgaagttc atctggttgg cgcttatccg
     5281 gtaacgccaa ttaatatcgc gattgaactg ccggaatttg acccgagcgt ttataacgat
     5341 gccattcgtg gccaacattt gctggcaatg aaagctctgc ggcagaaatt cggcattaat
     5401 gaaaacatga cgcacgtaga aaaaggtctg cccgaagagg tgattcctga tttggcggag
     5461 cacttacagg cggggattgt ggttctgggc acggtcggac gcaccggtat ttcagcagca
     5521 ttccttggca acacggcgga acaggtgatt gatcatcttc gctgcgacct gctggtcatt
     5581 aaacctgacc agtatcagac acccgttgaa ctggatgacg aagaagacga ttaacgactc
     5641 ttcaataact gacaaggccc gagttatcgg gcctattttt tacttactgc tcttcgccgc
     5701 caaggaaata aattcccaac gggattgcca gcaacactgt aaagaccagg ctataaacaa
     5761 aaatcatcga tgactgaatc acatacattg atgtcgtcca ctcagacagc ggaatattgt
     5821 actgttctat aacgccgccg atggtcgcgc gagtcacaac cgacgccgca ataatgaaaa
     5881 ccgccaacaa accaaagaga aatttcttcc ctttcgctga cttcagtttc gccataatca
     5941 tgatgtaatc cttgtgcaga caaatttctt gttacctaaa cgtaacaata ccatgacatt
     6001 ttaccgttgt ctgctctgaa aatctcacct cttagctgga tttacaatca acgcattgat
     6061 tagttagcta atgttgctac ttccttccct gactattgat gatctgaatg aattgtcgcg
     6121 gtaaaatagt cccgccaggg gtttccggca tgacgacaat tatctggagg agcctgataa
     6181 aacaatgatc gctgaactgt ttacaaataa tgcgcttaat ctggtcatta ttttcggtag
     6241 ctgcgcagca ttgattctga tgagcttttg gtttcgccgt ggaaatcgta aaagaaaagg
     6301 atttttattc catgcggtgc aatttttaat
//
LOCUS       DADTFG010000204         6201 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.205, whole genome shotgun sequence.
ACCESSION   DADTFG010000204 DADTFG010000000
VERSION     DADTFG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.205"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..757)
                     /locus_tag="J1138_004318"
     CDS             complement(<1..757)
                     /locus_tag="J1138_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA8456023.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSYSETVFIATPPLFILKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPTLENSHSVKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNMSYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCK
                     SQGVCEIAWQTLDIYNNVLPILKVKLNPSQLDVT"
     gene            complement(723..1295)
                     /locus_tag="J1138_004319"
     CDS             complement(723..1295)
                     /locus_tag="J1138_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA8456024.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTNSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEDGSVEGLALTIFDKNKGSISNDVKSMGFSLTSSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            complement(1246..1809)
                     /locus_tag="J1138_004320"
     CDS             complement(1246..1809)
                     /locus_tag="J1138_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA8456025.1"
                     /translation="MNLIFSKYSVFFIFFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(1809..4403)
                     /locus_tag="J1138_004321"
     CDS             complement(1809..4403)
                     /locus_tag="J1138_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBA8456026.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALASSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHLKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELDNPV
                     SYATLECR"
     gene            complement(4440..5192)
                     /locus_tag="J1138_004322"
     CDS             complement(4440..5192)
                     /locus_tag="J1138_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA8456027.1"
                     /translation="MKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNMFVPPKSDI
                     TVSSPVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(5293..5997)
                     /locus_tag="J1138_004323"
     CDS             complement(5293..5997)
                     /locus_tag="J1138_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA8456028.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDATQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT         1852 a         1369 c         1136 g         1844 t
ORIGIN      
        1 cagtaacatc caactgtgaa ggatttaatt ttactttcaa aattggcaat acattattat
       61 aaatgtccaa tgtctgccat gctatttcac aaaccccttg cgatttacac catgattgtt
      121 ggcgatggct aaatggtgcg accataccac catctataac tttaccatca acatgtaggt
      181 tatagagtgt cacatatctg gggcctggat tataaacggt aacaatttca ttttttcgtg
      241 cccagcgtaa tttttgatat gacatattcg ggtcttcaga caaatttgat ggccgataga
      301 atattttcat acgattgcgg actgctattt gcaattgatt atcagttttg actgaatgac
      361 tattttccaa cgttggtaat gctgcaacat tcagtataaa taaactttcc cggtcggcag
      421 gtaaactgcc accaacacga ttaattctta tttttgtatt tttgtcaggc ttcaaaataa
      481 acagtggcgg agttgcgata aataccgttt cagaatatga gggcaaagat tcaaccgttc
      541 ccgtcaactg acgagcatca tcaggggaga ctgcaacctt gactaacata tctctgtctg
      601 aagtgttttt gagttcaaca gatattgagg actgattttc cggatacgca aaacgcgttc
      661 caccgatcac taagcctgct tgtgctgtag agaataaaag ggaaaataat actatgacta
      721 ccttaatcat aggtgacaat aaacgatact gacgcatttg catcccctgg ttggacatta
      781 ttttttaatg ctttgtaagc cgcaaaaaaa tgcaatatat tatcaactga tgatgtaagt
      841 gaaaaaccca tgcttttaac atcattacta atactgcctt tgttcttatc aaaaatcgta
      901 agtgcaagcc cctctaccga gccatcttcc agcttatata aactggcatc ttcagaaact
      961 gtattgccgg taaactttat atacgcgttg ccaacatcac taccacagcg ggttaacgtt
     1021 atggaaaaat aaaccttttc agtaacatcc cctgcactcc ttaccgaact actgggtacg
     1081 tctggcatat taactgtaaa attttttgac tcatcagaaa taatgcaggt tttttcggta
     1141 attaaccctt caatacgaac cactccggca tgagtgatat catcagcaaa agaatttgtt
     1201 gatattataa acaaaaataa actaatgaat atcttcatat ctccctcact gataatcgat
     1261 atcaaaatac aacagagcat ttgcttgccc ggcatgtaca ttttcctgag ttgatttata
     1321 gcgaagttta aacgtcaaat tagcatcacc gttttcattt aacactaaat cacgaaaacc
     1381 tgattcacca ttaatggcaa tcgccacatc attgttatcc aatatttcta ttcccagtcc
     1441 agtcgcacta tctgcaccgg cggagagacg cagtaatgcg ctattaatgg catctgcatc
     1501 accagtaaac cgcacattgg cgctcacacc agaactataa acagtacttt ttgcagtcgg
     1561 gcatccttgt acattaatcg taaacgtttg actcggagaa acatcaccta catttaaaaa
     1621 ttcattagca tcataagtgc ccatttttac cgactgatct ttacttccgg tagtaatgtc
     1681 acagcttttt gccaacaaaa tcccagtaaa tgtaacaaca actgaatcat tggcctgccc
     1741 tgagagcgga aaagcaaaaa aagcgaaaaa aataaaaaat acagaatatt tagagaatat
     1801 taaattcatt agcggcactc cagagttgca tagctaacag gattatccag ttctgtttca
     1861 gggagcttgt aattagcctg acatttttgc tgaacaccgt ctccccactg gatattaata
     1921 acaccttcta aaggcaatcc tgagagataa agttgtccat tatcgccgac aatcccggta
     1981 atttgactac tgtttttaat ctgagcgctg gctgtagcac cgaatgggac tgatttatta
     2041 cgataacgaa ctgtgaacaa accacgaata ccgatatgag cagcaaactc tgcacgaact
     2101 aacgcacctt ttcccggaat aacttcagtg gaagtgatat ccagttcagt atgcatattc
     2161 attgacatag gatctaatga aatattatta cgtctatatt ctgttgcata aggaacaact
     2221 gcatatccac gccagtcagt ggatattcct gtcccccgct gaacatccac attattcgct
     2281 ccaggcgctt ttatcaaaac attagtttca cctaatggtt gactcaacgt aagaccattt
     2341 tcatgtacaa caagagcacc actgatgcca tagtttattt gttgattacc ttcattatcg
     2401 taactgtagg cggaattcaa ggaaccatat gccccacgat aattaacacc aacagaacca
     2461 ctactgctat tatcctgatt agcaaaacca tgttgtatgt tataactgag attatgctgc
     2521 tccaacagag aaccagatac ccccgtatat tggtttacct gtccgtggtt atcctgacta
     2581 taaccatacg ttgcagtcat ggcattcgat gatgagcgcc catcattcgt ggaagataac
     2641 caattactta taggtaatga gacatttagc gagacaattt tatccgcatt tgtccctggt
     2701 gatttactgt aattccacgc aacatttaga tagataccct taatactggt gttataacca
     2761 acttggtata aagaatcttt tttatcagta ttccagtatg tttgctgact agccgataaa
     2821 tacatagacc cgtagttacc gaaaggttgc gatacactag cctgaaattt tccacgtttg
     2881 ctataacgca agttataaaa atcattccag ttataaacgt aatctttatc atctaacgtt
     2941 tttggatcaa caacagtccc tgacatttgt tggtatgtcg tatcacttaa agtgtaaaag
     3001 ccctgggttg aataacgata gccaataata tggaatgctg ttcctgtctg aactaacgat
     3061 ttggaataaa gaaaacgata agattgccct tgatgtttgc tcccatccac aagcgaactg
     3121 ttagcctgag taacatcaaa cgatgttgca ccaaatacac ccagattcag gccaaggccg
     3181 agcgccaacg cccggtaatc ctcagagaat tgtgccccac cataagccgt aatatcccag
     3241 ggtaaacccc aaattaattc cccctgaaat acataaggac ttttctggtt atagctattg
     3301 cttcgatatt ggccaaccgt agtagaatat tttaaatgac cttctcgttg caggatgggg
     3361 acagcggcat aaggtacggt atagacagtt tctgaattat cagactcttt gatggtaact
     3421 tccagatctc ccgcagatga tgtcgggtta agatcgctaa tctcaaacgg tcccggcggc
     3481 atataggttt gataaatgac ataaccattc tgtttgattg ttacctgagc gcgacttttc
     3541 gcaattccgc gcaccgtggg agcgaaacca ctttgactat cgggcaacat attttcgtca
     3601 gattccagtt ttatgccacg aatactaaca ctgtcgaaaa catctgatga tgaccatgta
     3661 tctcctagcg ttaattcact tctgaaagga ataataacgc gctgcaacgt actactgata
     3721 tgctccagtt tacctgctga attactccca cgactccagg ttgaataatc gcgaaaacgc
     3781 catgcaccaa tattgatccc agcgcgaaga tttaaataat agttatttga agagtaatca
     3841 ccacgatcgt tattaccaga aaagtcataa ttcagcaaaa aagctgtaat accgttatcc
     3901 cactgttctg gtggaatata tccgcgagcg tcacgtttta ttatcgcctg tggaaaggtc
     3961 agtttaagtc tctgtgcatc cagctccggc atgtaatgca catcgggaaa ccagatacgc
     4021 aaatcaaggc aactattttc agatgaagcc aaagcctgct ctggcagcgc atttttgtta
     4081 attccaaggc ttaataagtc agcaagtgaa aggcagggaa tcagttgatt ctcatcgtct
     4141 gctttaaaag ttacagtacg agagagtaaa ggggaatcat tgacccaaac ttcgacttca
     4201 tagctaccag gaagataagt catccctttc tcgaagcggg ataagtcagc aacagcttca
     4261 gtatcacccg acagaaaagc agggttaaag cgcattcccg cgattgaaca tgcaggcata
     4321 ataaccagaa gaagcggcac aacccgtttc atctgctttt gtgccgccat ccaatattca
     4381 catcttttga gctttaggtt catatccctg acctgtgttg catctgaact attccgttgt
     4441 tactgcatta ttcctttctg ccgctccgtc gtagcgccaa agtcattaat tgtttgataa
     4501 gtaatttcac cagccaacgt cactggcgac gagaccgtaa tatcactttt cggtggtaca
     4561 aacatattgg gcaatttact cccttgtact ttaattcctg tcagtgtcag gtaataaggc
     4621 gaaggattga taacttttag ttgtttgccc tcatacttaa accgcaacat atttcgtgct
     4681 tttcctggtt gcacaggtag attttccggg cgataaaata gcttgatttt gttttgtaat
     4741 gccaactgta aggtattctt ctgtgtattt ttctcatctg ttggcggaat tgctttaaca
     4801 ttcatccaat acaaagtttc acgatcggtc ggcaaattag tcttaccaag aaacatgata
     4861 cgtaatgaag cttctttacg cgttggtaac ataaagaggg gaggagtgat aacaaaatcc
     4921 tggctctttt tttccgtttt actgtcatca acccatgatt gaattaaata actggtgttg
     4981 tcaccgctat tttgcacact gagtgttatt tgcttacttt ctgccggata aatcaatcgc
     5041 gttacgccaa ggccaaaccc gcccgcggac gcattattaa taaataatat cgatagtaaa
     5101 aaaagaatcc ctgagcgtat aatattttgt gatactttta attcatccct gaatccataa
     5161 acccattttt tatttccaat aatacatttc atacagcatc cttgttgatg aaataacacc
     5221 tgttattcat actctattaa ataatttcaa tctcaggtta caactttttg taacctgaaa
     5281 tcgtatttat atttactcgt acagcaccac aaaacttgct gttgacttaa ctacaccagg
     5341 tgcaacagtg ctaccgtttt gagcataacc tgcagtaaag gccgcgctga aatcatgggt
     5401 cgtagcattt cctggaattg tataagcggc ggtggaggcg ctaccctctg ccgcagtagt
     5461 attcaggtta atcagaccat tattcgtcgt acctttggag taaattcgaa tccctacacc
     5521 agtcgccccc gcattgccgc tgttgtcagc atccgttgat ccttctactt cgttagcgag
     5581 cgttttatta gcgctatctg cagtaccggt aaaacggaac tcaattttct gatcctctgt
     5641 attgctacat tcggtcagat taatattgaa atcggtctgg ttgctcgccc cctgtgcttc
     5701 tacgtcgata ccagtgaaaa ggttggaagg cacaaccggc aggacaactt caatctgttt
     5761 tgaatcggtt gcgaccttac aggtagcttt cactacacgg ccagtaaagc ggattttccc
     5821 ccccattgaa tcaacgttgt catccatatc agtgagcctg ggataatcaa cggtgctcca
     5881 gtcagtatcc gccccaccaa attgtgttgc atcgccgggt gctgcggtta ccgcagtaga
     5941 catcagcata cctgatgcca gtaatactgc agataaatta gataaattga atttcattta
     6001 tacctttcct tttgttaaaa aatccaagca acattggtgt ttttacattc tgcgttaata
     6061 agttatatct cacaaaaaaa ataagctgac gttggctaat tccagaaggt tctttagaag
     6121 agtaattgag tgagtaatat tatatatgct gcttagtaat gtacctaaaa taatccaaag
     6181 ccgcaataag tgagtttata c
//
LOCUS       DADTFG010000205         6017 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.206, whole genome shotgun sequence.
ACCESSION   DADTFG010000205 DADTFG010000000
VERSION     DADTFG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.206"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            107..640
                     /locus_tag="J1138_004324"
     CDS             107..640
                     /locus_tag="J1138_004324"
                     /inference="COORDINATES: protein motif:HMM:NF018826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBA8456029.1"
                     /translation="MLNLYITHSDLSPPDCVSTENNRLTSVISVLVSLQDNNIISVTD
                     YLKKHGISHCFYFLSTGNIRFTTHNRSMITVQTKKSPVYINYRANNIWTKLVIKKHLS
                     GNIPFFRSQFTLSRAGIFKYVLDVNMKQCQYYSVQNNILHSQFIIPVSSTIAPDRRPC
                     GCSPHPVYAAKPGVHRF"
     gene            complement(797..982)
                     /locus_tag="J1138_004325"
     CDS             complement(797..982)
                     /locus_tag="J1138_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456030.1"
                     /translation="MRELKMKLCALMLPLVVSACVSTPPVCPPQTKPPAPPAWVMLPA
                     PDWQTPLNGIISPSESG"
     gene            complement(1501..2034)
                     /locus_tag="J1138_004326"
     CDS             complement(1501..2034)
                     /locus_tag="J1138_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA8456031.1"
                     /translation="MSKKLRYGLSAVVLALIATGASAPEILDQFLDEKEGNHTTAYRD
                     GAGTWTICRGATRVDGRPVVPGMKLTKEKCAQVNAIERDRALAWVEKNIRVPLTEPQK
                     VGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(2039..2254)
                     /locus_tag="J1138_004327"
     CDS             complement(2039..2254)
                     /locus_tag="J1138_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA8456032.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(2332..2637)
                     /locus_tag="J1138_004328"
     CDS             complement(2332..2637)
                     /locus_tag="J1138_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HBA8456033.1"
                     /translation="MTEMKKLVTADAVKSALRTEEVRGTLKESIRKTFEAQIDADVEA
                     ILDELTGPAEEASAPQAGDGENTADSPVENSSEQPETQPGGEEQPADVLQPEPATML"
     gene            complement(2661..2837)
                     /locus_tag="J1138_004329"
     CDS             complement(2661..2837)
                     /locus_tag="J1138_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HBA8456034.1"
                     /translation="MSFIQQIQLYFCTAVCALYLVSGGYKIIRNYIRKKIDAMAAEKI
                     SAGQSAEPKSTPLP"
     gene            complement(3000..4943)
                     /locus_tag="J1138_004330"
     CDS             complement(3000..4943)
                     /locus_tag="J1138_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBA8456035.1"
                     /translation="MTFKHYDVVRAASPSELAERLSQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSGDSGTVMSTVSEPEYYFVVVLAGQSNGMSYGEGLPLPDSF
                     DRPDPRIKQLARRSTVTPGGTPCKYNDIIPADHCLHDVQDMSGINHPKADLNKGQYGC
                     VSQGLHIAKKLLPYIPQNAGILLVPCCRGGSAFTSGPDGSFSEASGASADSSRWGVGK
                     PLYQDLVSRTRAALAKNPKNKLLAVVWMQGEADLASGSQQHNGLFTTMVQQFRTDLSP
                     LAAQCVSGNAATVPWICGDTTYYWKNAGTDKYEAVYGGYKGKEAQNIFFVAFLTDENG
                     TNTPTNAPTEDPDIVAIGYYGAASRTQGSFVSTQRDSHFSSWARRGIISDRLASAILL
                     HAGRTTELMGGQTVTSPDEKPSPDAPSKPSTPPADTTTMSTLFAYRASESEGQLAPQG
                     WTAGGGKAQTVDDAGASGGKAMKLTKETGKSSWYLEHDAGNGADLLGKGGLVSCRFKV
                     DGALTANQYALALYWPVSGLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQ
                     NTKLGSFGAFNNEWHTLGFRFAGHNSIEVTPVIDGQDGTPFMLSQSPVGTFRADKLRV
                     TDITNGATYPVLIDSIVVEVNNA"
BASE COUNT         1475 a         1676 c         1332 g         1534 t
ORIGIN      
        1 ataatcaccc tgcctgacta tgcggaaata acaaatagac atttcctttt cagttcatta
       61 ttttctcgcc gctccataat cttcagtcag acatcgtctt ttttacatgc ttaatcttta
      121 catcactcac tctgatttat cgccacccga ttgtgtctcc acagagaaca accggcttac
      181 ttctgttata tccgtacttg tttcattaca ggataacaat attatttccg ttaccgacta
      241 tctaaaaaaa catggcatct cgcattgttt ttacttcctg tccacaggga atatacggtt
      301 tacaacgcat aaccggagta tgataacagt gcagacaaaa aaaagtcctg tttatataaa
      361 ctacagagca aacaatatct ggacaaaact ggttattaaa aaacatttat ccggaaatat
      421 tccatttttc cggagtcaat ttactctttc ccgggctggc atatttaaat atgttctgga
      481 tgtaaatatg aagcaatgcc agtattattc agtgcagaac aatattctgc attcgcaatt
      541 catcattccg gtttcttcca ccatcgcacc ggacaggcga ccatgtgggt gcagtccgca
      601 tcctgtttac gcggcaaaac ccggtgtgca tcgtttctga ttattcccgc acactcacgc
      661 agaggagttt ccccgtcggg ctgcggtctc tgttaatgcg ggaatacggc gacaatacaa
      721 cgcactgaga aaaactattt cacgcactga gtactgatat attcctgtgc cccttccagc
      781 tgcttctgca tcgtcatcag ccgctctctg agggtgaaat aataccgttg agcggcgtct
      841 gccagtctgg ggcgggcagc attacccacg cgggcggtgc cggtggcttt gtctgtggcg
      901 gacacacagg tggcgttgac acgcaggcgc ttacgaccag cggcaacatc agcgcgcaga
      961 gtttcatttt cagctctcgc atcggctaac tcctttgtgt attttgcatc aagttcagca
     1021 acatcacgct gacgctgctg catgtcagta atggtcgcgt tcgcctggct gagttttctc
     1081 ttcgcatcat cccgctgcgc tttataagta atggcgttat cgcggtaatg gtctgttgcc
     1141 agccacagcg caccgcaggc caccagcaat gcaataatga ccacataaag aacgcggttc
     1201 atatcaccac cagcgtatct gaccaataaa ataaccagag gccataatca caaacaccag
     1261 ccagataagg atgaacttcc aggtggataa tttttcagcc atcactcgta tctcccgaat
     1321 cagtttgcta aaatcaaaca cactttctcc tttgactttt ccggagtctg gaaatacaag
     1381 aaccccgctt gcggccaaca aacggggttt tacttttatt cactcacgtt ttgccagttc
     1441 tcagtatttc gtgttatcca ctcacgctgg ccactgccat ttttcagcaa aatattctgc
     1501 ttatctgtcg ataccccagc acgccagcgc actctcctgg tcacgccggg atacctgacc
     1561 gtagcagtta tttgagcgga tacggcagtc tctgccaccg tcctttatcc accagcgaat
     1621 cgcttcgcag gcaccttttc gatcacctgc attaattcgt ttataaaacg tcgacggaaa
     1681 acacttaccg ggaccaatgt tgtacggaca gaatgacgcg atccccactt tctgcggttc
     1741 ggtcagcggt acccggatgt ttttctccac ccatgccagc gccctgtcgc gttcaatggc
     1801 attaacctga gcgcattttt ccttcgtcag cttcatgccg gggactacag gtctgccatc
     1861 cacccgggtg gcaccccggc agatagtcca ggtacctgcg ccatcacggt atgccgtggt
     1921 gtggttacct tccttttcat ccagaaactg gtcaaggatt tcaggcgcag aagcccctgt
     1981 agcaatcagc gccagaacga ccgcagataa accgtagcgg agttttttac tcaccagttc
     2041 attctccccg cgctgccttg cgccggtcct ctctgatttt aaaatacagg ttggtcaggt
     2101 acgtcagcag accaaacagc agactcccca gcacgcctat tgccgcccac tgagacgggg
     2161 ataccctgtc cagcaactgc agaaaccagt atcccgtccc caccgcagac gtggtgtatg
     2221 acacacctgt tgtgattttt tccatctggt acatatcccc gcctcccgtt caccggaagc
     2281 gcacgataac aaaagaccac cgcgatatgc tggtggcctg tgcgtcatgt atcacagcat
     2341 ggttgctggc tccggctgca gaacatcagc cggttgttcc tcacccccgg gttgtgtctc
     2401 cggctgttcg ctgctgtttt ccaccggaga atctgctgta ttctcaccat cccctgcctg
     2461 tggcgcagat gcctcctcag caggtccggt aagctcatcc agaatcgctt ccacatccgc
     2521 atcaatctga gcctcaaacg ttttacggat agattctttc agcgtaccgc gaacttcttc
     2581 agtgcgcagc gcagacttca ctgcatcagc agtgaccagt tttttcattt ctgtcatggt
     2641 tttctcgctg tgagatgtga ttacgggagg ggcgtggact ttggctctgc tgactgaccg
     2701 gcactgattt tttcagccgc catggcatca atctttttac ggatgtagtt gcggatgatt
     2761 ttataaccac cactcaccag atacagtgca cacacagccg tacagaaata cagctgtatt
     2821 tgctgaataa atgacatata cccttacctt taatattgac tacagaaaca gttaccggtt
     2881 aaaaagtaac tgcgtaaaca atggccttct ttaattgagt tgttgtattc cttaatgggt
     2941 ttggaatatc acccgccagt caacaaattg ctctttgact ggcggttttt ttaccctgat
     3001 tacgcgttat tcacttccac cacaatgctg tcaatcagca ccgggtatgt cgcaccatta
     3061 gtgatatcag tcacgcgtaa cttgtctgcc ctaaacgtgc caaccggaga ctgcgacagc
     3121 atgaacggtg tcccgtcctg accatcaatg accggcgtca cctcaatact gttgtgaccg
     3181 gcaaaacgga aacccagcgt atgccactca ttattaaatg cgccgaatga ccccagcttc
     3241 gtgttctgcg ccgtgtttcc tttgtgatac atcacgttaa ggtctgtggc atcgctctgt
     3301 acgtagaacg atgccagcag gttatgaccg gcattacctt ccagagtaac cccttgtggc
     3361 agaccagaaa ccggccagta cagcgccagt gcgtactgat tagccgtaag cgcaccatca
     3421 accttaaacc ggcagctgac aagaccccct ttacccagca ggtccgcacc attaccggca
     3481 tcatgctcaa ggtaccagga ggatttgcct gtttccttgg tcagtttcat ggccttaccc
     3541 ccgctggctc cggcatcatc cacggtctga gccttaccgc ctccggcagt ccagccctgc
     3601 ggtgccagct gcccttcaga ctcagatgcc cggtaagcaa acagcgtact catcgtcgtg
     3661 gtgtcagccg gtggcgtgga cggttttgat ggtgcatcag gtgatggctt ctcatccggt
     3721 gatgttacgg tctgtccgcc catcagttca gtcgtgcgtc ctgcatggag cagaatagct
     3781 gaggccagac ggtcagaaat gatgcccctg cgtgcccatg aactgaaatg gctgtcacgc
     3841 tgtgtcgaga caaaactgcc ctgggtacgg gatgccgcac cgtaataccc gatagccaca
     3901 atatccgggt cttccgtcgg agcattcgtt ggcgtgttag tgccgttttc atccgtcagg
     3961 aacgccacaa agaaaatatt ctgtgcttcc ttgcctttgt agccaccgta taccgcctca
     4021 tatttatcgg tgccagcgtt tttccagtaa tatgtcgtat caccacaaat ccacggcacc
     4081 gtggcagcat ttccactcac acactgcgca gcaagcggag acaggtcagt tctgaactgc
     4141 tgaaccatgg tcgtgaataa accattatgc tgctgacttc ccgatgcaag gtctgcttct
     4201 ccctgcatcc agaccactgc cagaagtttg tttttcgggt tcttcgccag cgccgctctg
     4261 gtacggctta ccaggtcctg atacagaggt ttaccgactc cccagcgtga agaatcagcg
     4321 gaagcaccgc tggcctcact gaatgaaccg tccggcccgc tggtgaatgc tgacccgcca
     4381 cggcaacagg gcaccagaag tatcccggca ttctgcggga tataaggcag cagttttttg
     4441 gcaatatgca gcccctgact cacacaacca tactgcccct tgttcaggtc agctttcggg
     4501 tggtttatcc cgctcatgtc ctgcacatca tgcaggcagt ggtctgccgg aatgatgtca
     4561 ttgtatttac acggcgtacc gcctggcgtg accgtgctgc ggcgtgccag ttgcttaatc
     4621 cgtgggtccg ggcggtcaaa tgaatccggt aacggaagcc cttcaccata ggacatgcca
     4681 tttgactgcc ccgccagaac aacaacaaag taatattccg gctcactgac tgtgctcatt
     4741 accgtaccgc tgtcacccga cggcttcacc acaacaggcg tggtgacatc accctccgca
     4801 gcaatcgcct gcatcagggt ataaggcgta atagcaaccg gactgccaaa cggctgccag
     4861 ccttccttca gtttttgtga cagtcgctcc gcaagctctg acggtgaagc cgccctgacc
     4921 acatcataat gcttaaatgt cattgctcct cccggacggg ataatgtgtt aactaagatg
     4981 agagagcaga tgacagcccc ggccacatga aagttctgag agggaagtgc tgcctgctgc
     5041 tccattacac agattaatta tctgtctggt agtattcagc ggctgattag aatacttcac
     5101 attttgttat tttgtgcagc ccgttaccat tcgtagcggg cttttttagt ccataaaaaa
     5161 gcccctccgg agaggggcat actagctata tatccattta tcattggctg cgtggtgccg
     5221 ggtgcctccc ggtgagctca gcccggtgtc actgagcccg cacattctcg atccaagtta
     5281 tcgtcggaga taatacctga tacaccagac gcccctccgc acagggggat tcaccacgcg
     5341 cagagaagaa tactgaacac tattttctcc aggcaatatg caataacatg aagtgtgaga
     5401 tgacttacaa ttataaaaaa cagcctacag aagactccta aataaagcca tatcaaaatg
     5461 agtaacagga aaagaatcca gggaaaaatt tacgagcagg tattatccag caacagttca
     5521 gacgccataa cttattgccg atagctacca tcaataattt ctttcataaa aaagcccctc
     5581 cgaagagggg ctgacatacg catttgcttt catcattaat atggcgccgg gtgcctccca
     5641 gtgctccata actgattaat atggaacgcg gtttcattca gttgcattca tgaataacac
     5701 tctaatcagt ccgccccgcc gcttaggggg attcgccata taaattgaaa gtactgaaca
     5761 catctgtcac aaacaatatg caattgtatg aattgtgaga ttattatcaa taacacaaaa
     5821 ttatagctct agtacataaa cagcagatta aacaagaaca acaaaagtat tgaattgcac
     5881 ggtgccgggt gcctcccggt gaattctgca atgaccagac agaatccgca acttgctact
     5941 aacgctaatg cgaccaagcc taacagtcat tatgcccctc cgcacagggg gattcaccat
     6001 gcgctaaaaa aatacaa
//
LOCUS       DADTFG010000206         5806 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.207, whole genome shotgun sequence.
ACCESSION   DADTFG010000206 DADTFG010000000
VERSION     DADTFG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.207"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            14..1474
                     /locus_tag="J1138_004331"
     CDS             14..1474
                     /locus_tag="J1138_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA8456036.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLSKIEQLTGVRDAAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPGNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1510..1713)
                     /gene="ydfZ"
                     /locus_tag="J1138_004332"
     CDS             complement(1510..1713)
                     /gene="ydfZ"
                     /locus_tag="J1138_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBA8456037.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1891..2577)
                     /locus_tag="J1138_004333"
     CDS             complement(1891..2577)
                     /locus_tag="J1138_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA8456038.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2666..3412)
                     /gene="ydfG"
                     /locus_tag="J1138_004334"
     CDS             complement(2666..3412)
                     /gene="ydfG"
                     /locus_tag="J1138_004334"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBA8456039.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3549..5594
                     /gene="dcp"
                     /locus_tag="J1138_004335"
     CDS             3549..5594
                     /gene="dcp"
                     /locus_tag="J1138_004335"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBA8456040.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIT
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIASWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFEMRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
BASE COUNT         1451 a         1378 c         1492 g         1485 t
ORIGIN      
        1 ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacgctcc ctgtttatga
       61 tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc accgtgcgca
      121 tcagggtgtg tatgccgata ttcttgctac ggaacatttc agtgactggg gatattatga
      181 ggtcaattta atcggcggcg aacagcaaat tgccgattta caacagcaag ataatcttta
      241 taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg tcgttaaaaa
      301 agccttgcac gtacagattg atggcttaga aaccgtgttg gctgcgatgt gtgaaccgca
      361 aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc cggcgaccgg
      421 acagttaatg ctcgatcacc cgatggtcgt tgccgacgta caaaatcccc accagccgaa
      481 aactgcaaca ggggtgattg tcgaggcgct ggctcgccgt aaagcggcag gacttcccgc
      541 atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc gtgacgttgt
      601 cacttcctac gcgcaagctg ttgatgtaaa actggcacaa tggatcgaag aaaacgtgac
      661 tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata cgctgtcgaa
      721 aatcgaacaa cttaccggtg tgcgcgatgc ggcaggcgtt gcctgtgaac ctttccgcca
      781 gtgggtaata gaagataact ttgttgccgg acgtccggaa tgggaaaaag cgggagctga
      841 actggttagc gatgtgctgc cttatgaaga aatgaaactg cgtatgctta acggcagtca
      901 ttcattcctg gcgtatctgg gctatcttgc cggatatcag cacattaatg actgcatgga
      961 ggatgaacat tatcgtcatg cggcgtatac cttaatgttg caggaacaag cgccgacgct
     1021 gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgaac gctatagcaa
     1081 cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga aattgccaca
     1141 gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg atctgctggc
     1201 gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg gaaatccgat
     1261 agaaatcagt gatccactgt tacctgttat tcagaaggca gtacaaagta gtgctgaagg
     1321 gacagcgcgc gtccagtcac tgctggcaat taaggcgatc tttggtgatg atttaccagg
     1381 caatagtttg tttacgacaa aagtaacgga agcgtacttg tctttattag cgcatggcgc
     1441 gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc atcggcggca
     1501 ttcacacgct tagttcatgc cgagacgaat caggtccaga ggtgccagtt tctcttcaca
     1561 accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac cttcagtatc
     1621 aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc tgccagtggt
     1681 gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc tgatttacag
     1741 tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata ttttttgttt
     1801 ttgatcaagc tcacatcttt tttgatgcat gcatgagcca taaggcgggg cgaatagact
     1861 ctcaccccgg atgggatgag aggaaatgaa ttactcttcg ctaaaccagt cgctgttttc
     1921 ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca ttgcacgttc
     1981 taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct ggcgtaacag
     2041 catttctggt ggagaaacgt ggtcgaaact catatagcgc acgcgatcaa cggtcgcttt
     2101 caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca ggagttgatg
     2161 gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg caatgcgttg
     2221 ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc tcgccgcccg
     2281 acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca cctgggccat
     2341 ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc cgttttccgc
     2401 cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa cagaaacttc
     2461 tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc gacgaagaat
     2521 acgataaatt tgctgattga caggctgtgt gggattaagt tgcgtttcga cggtcattcg
     2581 ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac aagcaataac
     2641 ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg gcatagcttt
     2701 gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc gttgacaccc
     2761 accagacggc ttcgctgaca tcttctggtg tcaatgcaac ggtattttga taggtttttt
     2821 ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca cccaccagac
     2881 ccggttcgat gtcggtgacg cgcaccgccg taccatgcag gtccgtacgc agattcaggc
     2941 taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca tacggccagc
     3001 tacctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc atacccggta
     3061 agacggcgcg cgtcatatat accaggcctt tgttgttggt atcaatcatc gtttcccagt
     3121 cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccagca ttgtttacca
     3181 ggatatcaat attgctccac tcggcaggaa gcgatgccag catctcttca atagcggcgc
     3241 ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct tttaactcct
     3301 gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga ataaaacgac
     3361 gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc atctcactgt
     3421 tcctcaacgc ttttgtgtgt caataacata gcatgtgatg cttaagagcg ggtaatactg
     3481 gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct cgcgttcagg
     3541 agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg tatctggctc
     3601 cccattttga tcaaattgcc aaccatcact atcgcccggc attcgatgag ggaatgctgc
     3661 aaaagcgggc agaaattact gccatcgcgc ttaacccgca aacgcctgat ttcaacaata
     3721 ctattctggc actggaacaa agcggagaat tacttacccg cgttaccagc gtcttttttg
     3781 cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag ttttccgctg
     3841 aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg cgggtagatg
     3901 ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgt ctggtggagg
     3961 tgattcatca acgttttgtc cttgccggag ccaaacttgc gcaagctgat aaagcaaaat
     4021 taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag cgattactgg
     4081 cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca ggaatgagtg
     4141 agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat aacaaatggc
     4201 tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc gatcgtgcga
     4261 cgcgtgaaaa actgtttatt gcgagctgga cgcgagcgga aaaaaatgat gccaatgata
     4321 cccgcgctat cattcaacgt ctggtggaga tccgtgcaca acaggctaca ttacttggtt
     4381 ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca gaagcagcac
     4441 tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat gaattagcct
     4501 ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg tgggactggg
     4561 cattttatgc cgaacaggta cggcgggaga aatttgatct tgatgaggcg cagctcaagc
     4621 catattttga attaaacacg gtgttgaatg aaggtgtatt ctggaccgcg aatcagctct
     4681 tcggtattaa gtttgtcgaa cgttttgata ttcctgtcta ccatcctgac gttcgggtgt
     4741 gggaaatttt tgatcataat ggcgtggggc tggcgttatt ttacggtgat ttcttcgccc
     4801 gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca acgcttaatg
     4861 aaacgcaccc ggtaatttat aacgtctgca attatcagaa acccgctgcc ggtgagcctg
     4921 cgttgttact ctgggatgat gtcataacct tattccatga atttggtcat acgctgcacg
     4981 gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg cgtgattttg
     5041 tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta ttcgctcgct
     5101 acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag aaaatgcgta
     5161 atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc gcacttctcg
     5221 atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat gattttgaaa
     5281 tgcgggcgct ggtggcggaa aatatggatc ttcccgctat accgccacgc tatcgcagca
     5341 gttatttcgc ccatattttt ggtggtggat atgccgcggg ttattacgct tatctgtgga
     5401 cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga ttaacgcgtg
     5461 aaaatgggct gcgttttcgc gaggcgatcc tttccagagg taacagcgaa gatctggaac
     5521 gcctgtatcg acaatggcgc ggtaaggcac ctcagattat gccgatgctg caacatcgtg
     5581 gcttgaacgt ataaacacca ccaatggctg gcctggattt atcgccagac cagcctcgtt
     5641 atagcagtaa ttgcagcagc tgggcgaggg caaacttcac cgctaatgcg aatgccgttt
     5701 cgcagtcgcc agcaaaatta ctttttttgt atactcttcc gttataataa ttttcaaatt
     5761 tataaatctg attgttctga aatatgttta agactcttta atcttc
//
LOCUS       DADTFG010000207         5738 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.208, whole genome shotgun sequence.
ACCESSION   DADTFG010000207 DADTFG010000000
VERSION     DADTFG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.208"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(621..737)
                     /locus_tag="J1138_004336"
                     /pseudo
     CDS             complement(621..737)
                     /locus_tag="J1138_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            858..968
                     /locus_tag="J1138_004337"
                     /pseudo
     CDS             858..968
                     /locus_tag="J1138_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1045..2010)
                     /locus_tag="J1138_004338"
                     /pseudo
     CDS             complement(1045..2010)
                     /locus_tag="J1138_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2077..2827
                     /locus_tag="J1138_004339"
                     /pseudo
     CDS             2077..2827
                     /locus_tag="J1138_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            2910..3299
                     /locus_tag="J1138_004340"
     CDS             2910..3299
                     /locus_tag="J1138_004340"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA8456041.1"
                     /translation="MSDMQKNVTLGRRKGCPNYPPEFKQQLVAASCEPRISISKLALE
                     NGINANLLFKWQQQWREGKLLLLSPESPQLLPVTLDATAEQPESPAESPAPLSISCEV
                     TFRYGTLRLNGNVCEKRQALLIQELKR"
     gene            3296..3633
                     /gene="tnpB"
                     /locus_tag="J1138_004341"
                     /pseudo
     CDS             3296..3633
                     /gene="tnpB"
                     /locus_tag="J1138_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            3704..5213
                     /locus_tag="J1138_004342"
                     /pseudo
     CDS             3704..5213
                     /locus_tag="J1138_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            5264..5449
                     /locus_tag="J1138_004343"
                     /pseudo
     CDS             5264..5449
                     /locus_tag="J1138_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5569..>5738)
                     /locus_tag="J1138_004344"
     CDS             complement(5569..>5738)
                     /locus_tag="J1138_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA8456042.1"
                     /translation="KEVLLMFLYGLSGKDIANNLGVSMKTVSSYKKSLMNRLYVCNDI
                     QLFYKGRLLFR"
BASE COUNT         1483 a         1480 c         1379 g         1396 t
ORIGIN      
        1 tgtgtattta ttgttcatta atatactttc aaatcgtcgt ggaacaatga tccaacccac
       61 gtaatttgga cacaggtcta agcaaggctc tggttttcaa attattccga actgaggtcg
      121 ccacaccaac tgtgccatca tctgtgatgg cattgaccag ccaataacgt cacgcggtca
      181 taggtctatt accaccgcta gtacagccag ctttcatcag tacgcagata cgtgatatta
      241 ccctcccact tcggttctga gccttggtgt aaaaatcatg ctccagcaga ttttctggca
      301 caggcagatc gtgaacactg tagctaactg ggctgaactt tcgggaagct tttgccctca
      361 tccctaacgg cacaagctta ctgctactgt tattactgtt caagtggtaa ctctgagcac
      421 acaattcatt actcaagcgt gaagcaccgt aacactggac caagtaaaaa cctcgcagac
      481 actgatgtca cagtgcttac ggaactgctg gcgtgtgctt atctttgttc gccgctgaca
      541 ccacggatat caggcgctgt gaccatccgg aagcttcgat gctaaactca tccagatatt
      601 tttcaataaa agatagtctg ctttttatcc cattctgcta actggcgttt gagacgtgca
      661 atctcaatag acatctccaa ctcaccttca gaggaaatct gctgattttg ctatttactg
      721 cgccagttgc agagttttca ggacttcact gcgaaattca aatgaatact ggtaagattg
      781 taagtatccc gcataatcgt gccattcaca cttagagatc atccggcata atcagtctgt
      841 caacgaagga gatcgctatg tgtaaagccc gttttactga gcatcagatc atcgctgtga
      901 ttaagtcggt tgaagccgga cgagacgttg aagatgtcgg ccgggaggct ggtatctctg
      961 aggccaatat aatagtcagg ttccgattgt gcaggcgcaa cctgtcacta tttttcagta
     1021 cactgcaact cgtctggctg ggtactaact tccaactgcc tgatactcgg tatcatttct
     1081 cagcatcgac cagattattc tcgcgttctt gttagcgacc gccacggtcg ctttattaaa
     1141 ccagcgccgt tcctttaact agttaaccca ctgatgcaag cagccaattg ttattcatat
     1201 caactctgac gacagcgcgg gcaccatgaa taaaaaatgt acgcaaatgc ttgtcgcctt
     1261 ttttcgtcat attcattagt acctgcttgt tgccactaga gtgctgtcgt ggaaccaggc
     1321 caagccatgc ggcaaagtga cgaccatttt taaattcagt tcctttggca atgctctggc
     1381 aggcttctga ttgcatgaac acagcgtcaa tttctttatc aaaaaagttt atacggcggc
     1441 caagatcgtt aaagagatca taaagctctg caaccgttct gcgcatacgg gcacttaacc
     1501 cgttttctgc atcctcaagg ataagcagaa cggcacggcg aactcttgag actgccgcac
     1561 caattgttat tccccgatca agcagcagct ctcggatttg acagactatc gcagtacgat
     1621 gattgacaat acgctgtctt gcacggtgta gcccctcaat atcctgctgt tcagggcttt
     1681 tcggtggaac aaactgcatc gtaggttgca tcagagccac agctatcgcc tgcgcatcgt
     1741 taccgtcatt ttttgcccac ggacaaaagg ttttacatac tgggggctga tgacttttac
     1801 attatgcccc agtttctcaa actcacgctg ccagtaaaat gcacctgtag atgcttctat
     1861 accggtcaga cacgccggaa tattcgacac cgtctggagt aactcttttc ggccggtacg
     1921 ttttgaataa aacggtttac ctgtctagtt taacccgcag agctgaaaga catttttagc
     1981 cagatcaata cccagaaata cgatattcat agtgattctc ctgctgagca catttcgtac
     2041 agttaaccgc agaaggagga ggtagtccat cccattaact ggaagtctag atacggcggt
     2101 atggcatctt ctgatattaa aaagatcaag gatcttgagg acgataacag gcatcttaag
     2161 cagatgtttg ccgatctcag tcctgagaac cgggcgctga aagatgttat cgaaaaaagc
     2221 tttaaaacca gcctttaagc gtgagccggt cactcatctg ataacgacgt tcggactcag
     2281 tattcatcaa gccagccaga gtctcaatct gagcagaacg gtttaccatt accgcccgga
     2341 tatcacgcgt gatgagcccg ttattgccgc attgcaggcg gaggctaaac gcccacgaca
     2401 tggttttcct aaactttacc tagttctgcg gcggcaggga tatccgtgga atcacaaaag
     2461 gctccaccgt atttagtgtc tgctgaaact gaattttcgc cgtaagggca aacagcggtt
     2521 gccggtacgt aatccatcac cattggccac gctgtcagcg ctgaaccaga gctggtctgt
     2581 tgattttatg catgatgctc tggcctgtgg tcgtcgtttt cgtacgttca atatcgttga
     2641 tgactttaac cgtgaggcgt tgtcgattga aatcgatctg aatctgccag ctcagcgagc
     2701 gattcgtatt ctcgacagga tcgcggcaaa ccgtggcgct atccggtcat gctgcgcatg
     2761 gataatggtc cggaatttat ctcactggcg gcagactgaa tggacagaga ggcacacagt
     2821 aaaactgtaa gcgtacagcc tgaaccgtct ggtcagaatc tgacgaatta gacaaagttg
     2881 tgtccacaaa ataagtagtg ggaaccaaag tgtcagatat gcagaaaaat gtgactctcg
     2941 gcaggcgaaa aggctgccct aattatcctc ccgaatttaa acagcagctc gttgctgcct
     3001 cctgtgaacc aaggatatcc atctcaaaac ttgctcttga aaatggcatt aacgccaatc
     3061 tgttgttcaa atggcaacaa caatggcgcg agggaaagct gctattactt tctccagaga
     3121 gccctcagct acttcctgtg actctcgatg caacagccga acagccagaa tcgcccgctg
     3181 agtccccggc ccccctcagt atcagctgcg aggtaacgtt ccggtacggg acgctccgcc
     3241 tcaatggcaa tgtctgcgaa aagcgccagg ctctgctgat acaggaactg aagcgatgat
     3301 cccgttacct tcctggacca aaatctggct ggttgccggt atcaccgata tgagaaatgg
     3361 cttcaacggc ctggctgcga aggtacagac tacgctgaaa gacgctccca tgccacccat
     3421 gttttcattt tccggggccg cagcggcagt caagttaaac tgctgtggtc caccggtgac
     3481 gggctgtgcc tcctgaccaa acggcttgag cgtgggcgct tcgcctggcc gccaacccgt
     3541 gatggcaaag tgttcctcac gcaggcgcag ctgttggaag gtatcgactg gcgacagcct
     3601 aagcggctgt tgacctccct gaccatgctg taaatctctt tatcctggtt gtcacagaat
     3661 aagcccggta aaatacgggt ttatgacgac atagtctgac gacatcttcc tgctgaaaca
     3721 gcgcctagcc gaacaagaaa cgctgaccca ggccctacag gaaaagctga gcaaccgggt
     3781 gcgcgaaata gaccatctgc aggcgcaact ggataaactc cgccggatga ccttcggcaa
     3841 tcgttccgaa aaagtctccc gccgtatcgc ataaatggaa gccgatctga accagttgca
     3901 gaaagagagc gatactctga ccgttagggt gtatgacccg gcagtacagc gtccgttgcg
     3961 tcagagccgc cctagtaagc cgtttcctga atcactaccc cgcgacgaaa agcgactgtt
     4021 gcctgcgacg ccgtgctgcc cgaactgcgg cggttcacgg aactatctgg gcgaagatac
     4081 cgccgaacag ctggagctga tgcatagcgc ctttcgtgtt atccggacaa tacgggaaaa
     4141 catgcctgta ctcagtgcga tgccatcgtg caggcaccag caccttcgcg gcccatcgag
     4201 cggggtatcg ccggaccggg gctgctggcc ctcgtgctga cctcgaagta tgcagccccc
     4261 ccattgtatc gccagtcaga aatatacgac cggaaaggtg tggagctgag ccgttcactg
     4321 ctgtcgggtt gggtggatgc atgctgccga cagcagtctc cgctggaaga agcgcttcat
     4381 ggctctgtca tgactgacgg caaactccat gccgatgata ccccggtcca gtactgctgc
     4441 cgggtaataa gaagacgaag accaggcggt tgtgggcgca tgttcgtgat gaccgcaatg
     4501 ccggctcagc gttggcacct gcagtgtggt tcgcttacag cccagacaga aaaggcatcc
     4561 atccgcagac tcgtcttgcc tgcatcagcg gtgtgctgca atcggatgcg tactccgggt
     4621 tcaacgagct gtatcgcaat ggtcggataa cggaagctgc ctgctgagct catgtccgcc
     4681 gaaagatcca cgatgtgcac gttcgcatcc cgtcagcact gacggaagaa gtcctggggc
     4741 agatcggtca gttgtacgcc atagaggcgg atataagggg aatgccgaca tagcagcggc
     4801 ttgctgaacg tcagctaaaa acgaaaccgc tgttgaaatc cctggaaagc tggttgcgtg
     4861 aaaagatgaa gaccctgtcg caacacacag atttggcaaa ggcgttcgcg tacgcactta
     4921 accagtggcc ggcactgacg tactatgcgg acgatggcta ggtcgaagca gacaacaaca
     4981 tcgctgaaaa tgccctgcgg gcggtcagcc tgggtcgtaa aaacctcctg ttcttcggct
     5041 ctgaccatgg tggtgagcgg ggagcgctgc tgtacagcct gatcgggacg tgcaaactga
     5101 acgacgtgga tccagaaaac taccttcgcc atgtgcttgg cgtcatagct gactggccgg
     5161 tcaaccgtgt cagcgaactg cttccgtggc gcatagcact gccagctgaa taacacatcc
     5221 ctgtcaatac ggccctcgct gtgcgcttac gtaaaactgg attatccagc caagtaagcc
     5281 gacgtagaat gctttcatcg agcgttttaa caggacatac cgtacagaaa tactcgattt
     5341 ttatctgttc agaacgctga ataaagtgcg ggaaatcacg gagaaatggt tatcagaata
     5401 taactgtgaa cgcccgaatg aatcactgaa caatatggta ccagagtagt accgacagca
     5461 ccattattta gccgagttgt caaaaaatac ctagaactaa aacgggcctc tttataagat
     5521 cattcgacat gcagacccca taaatagtct caagcgttcg gttagcgctt atctaaataa
     5581 caatctacct ttgtaaaata actgtatgtc attacagaca tacagtctat tcattaaaga
     5641 ctttttataa ctcgaaacag ttttcattga aacaccgagg ttattagcaa tatcttttcc
     5701 acttaaacca tataaaaaca tcaataggac ttccttct
//
LOCUS       DADTFG010000208         5713 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.209, whole genome shotgun sequence.
ACCESSION   DADTFG010000208 DADTFG010000000
VERSION     DADTFG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.209"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..683)
                     /locus_tag="J1138_004345"
     CDS             complement(<1..683)
                     /locus_tag="J1138_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBA8456043.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTR"
     gene            1913..3397
                     /locus_tag="J1138_004346"
     CDS             1913..3397
                     /locus_tag="J1138_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBA8456044.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(3719..4321)
                     /locus_tag="J1138_004347"
     CDS             complement(3719..4321)
                     /locus_tag="J1138_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456045.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(4408..4686)
                     /locus_tag="J1138_004348"
     CDS             complement(4408..4686)
                     /locus_tag="J1138_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBA8456046.1"
                     /translation="MPVKVVRCFHDHTGWFNNIRRNILMITPVSLMDDQMVDMAFITQ
                     LTGLTDKWFYKLIKDGDFPAPIKMGRSSRWLKSEVEAWLQARIAQSRP"
     gene            5317..5515
                     /gene="hha"
                     /locus_tag="J1138_004349"
                     /pseudo
     CDS             5317..5515
                     /gene="hha"
                     /locus_tag="J1138_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
BASE COUNT         1467 a         1226 c         1488 g         1532 t
ORIGIN      
        1 cgggtcatca ggggactggc ggcatggtcg ggaagcgctg tcatgagcgc actgaccagc
       61 gtatccagtt cccagccggt gcgggccgat acggccacaa ccggatgtac gggccggaac
      121 agacggaata ccgcctccgt tttttcgcga atattctgtg cctgtgcagg agaaggctga
      181 ataccggcca tatcccattc atggcagggc tccgttttgt cggcctgcgt caccacaaac
      241 agtacccgct gatgtccccg gtgcaggatg tgtcgccaga aatactcatc cacagacagg
      301 gcacggtcat cggctttaat cagccacagt accaggtcca gttcaggcag aatgtcacgg
      361 tacagggcct catactctgc atccctgtcc cggctctcgc ccaccccggg caggtcagtg
      421 ataaccatgc tgtgaccgtg gccactcaga cggaagcgcc gtacttcccg ggtgccggcg
      481 tgaacatcac tgaccggggt gacctccccc tgaaacagtg cattacagag tgaggattta
      541 ccggccccgc ttttacccat aatgccaatc acgggttcgt gacgggtgag tttgcgcaga
      601 tgttccagga tgtgacggga aagcgagtaa ggcagggagg agagcggttt ttcaattgcc
      661 tcaatggcat cagacggatt catacatgta ttcccggtga aaaaagacaa aaccccgcgc
      721 accggaaagg tggcggggga ttcacggaga taatatctgt ctgaaattgt tttgaatcga
      781 tgggattgtt gcgtattaac atgaaggaga tcaggactta tccgttactg ggtgatctcc
      841 ttccatgaaa atacgccgtt attgttaaac agcaggctga tttctccgta aaggggaaaa
      901 tatttatcgg ctgcatgatt atcaggtgtg tctgtgttta ctgctgtgag tatttttcag
      961 tgtattgtgg gggagatgtc agtgggagat attaaattta cgtttataat tatcttactt
     1021 cttctgtgtg ttttttataa gagtgcacac gtaatgatat ctgttgatac catgtggtta
     1081 agtgtttccc ttcctggtca tgcgtaatga ttaaatgctt ttgtgacacc attcttccgg
     1141 tctgaatatt tatcccgttc ttttttattt gtgcagtgat actgtcggaa gagaattcag
     1201 taatgtgagg tgtcctggtc aggataatat ctgctgagat attattagtt gagtatgttt
     1261 ttactgagca aaagctggct tttaattcct gtactgaaac gctgtcgctc gtccggggtt
     1321 aactgaaact ggatgatgat gtaaaatgaa ggctgttttt gtgtttgtcg aagaagtgtt
     1381 attttaatcc attctcatca ttggctggcg attatacaga agtatttgaa gaaaaaatgg
     1441 tgcattttat ctgcgtcagc agggaggaag tattttattg atgtatcagt attaaatggt
     1501 ttttgttatt tataaatatg tcatcctgtc catagaatat tgtactcagc attcagtgca
     1561 ggaactgtag tcttctttat tttattcttc ttcaggaggg ggtgattcct gaaggcgtta
     1621 ttcccggtgt tctgtttatg attcttcccg gcaggaaacc ggtatctgca atacccggga
     1681 tgatgctttt tgttcgtacg tggttgtgta aaatttgaat agtggatgtg ctctgaaggg
     1741 aaggagggga cacacagatt cattttgtct ttcttcagca gcaaaacaca ctgtggttat
     1801 tatttaacca ttaaatcaac aaaaattttt acctcatatt gatttgactg ctatttttgc
     1861 tattaatagc cagaattgca gtgcttttat atctgtgtgt agtgaggtta ctatgtcatt
     1921 cggtcagcct tgtgatgagt ttcccctgag ttcgctgcct ccactcattc gtgatgccgt
     1981 tattgaagct cagcaaatta cgcaggctcc ccttgggctt gtggcagcgt ctgccctggg
     2041 cgctgtttca cttgtctgcc agaatctgat tgatgtctgc agactgaata cactgcgggg
     2101 gccggtctct ctttttttcc tgacgcttgc cgagtccgga gaacgaaaaa ccgcagtgga
     2161 caaattatta atgaagcccc tgtaccagca ggagatgcag ttatattcca gatacaaaag
     2221 tgaactggct gtctggaaaa acaaggaaga gcttttaaag gctcagaaaa aagcattgtt
     2281 gtcaaaactg aataaagaac tccgcaaagg cgcggatgag tcagaaaccc tcagacaact
     2341 ggaagtactt cagaaaaaca gtgcggaaga acctgtgagg tataaattca tttttaatga
     2401 tgccacgact gcggcaatta agaatcagct ttgtggtaaa tggcgttcag tgggtattat
     2461 gtcagatgaa gccggtatta tatttgatgg atataccctg tctgagttgc cttttattaa
     2521 caaaatgtgg gatggctctg tattgtctgt ggacagaaag aatgaacccg aacagatgat
     2581 tgaaaatgcc agaatgacgt tatccctcat ggtgcaaccc ggtctttttg accgatatat
     2641 ggagcgaaaa ggttctgttg ccagggacag tggttttctt gcccgttgtc ttatcagtaa
     2701 acctgcaacc acgcagggga aacgctttat taacggagct gtgattccgg gaggatcatt
     2761 aacggcattt catgaacgat taatggaact ggccagaggc agtattgaaa aatccagtga
     2821 ggatgaacgg tattgtctgc atttttcgcc tgaagcgcaa aaaatattta ttgagcacta
     2881 taatgttctg gagcaggatt tatccccttc cggaccctta agcccgtttc ggggacatgt
     2941 ttcaaaaaaa acagaaaaca ttgcaagaat tgcagcgtta tttcagtatt tcagctatgg
     3001 tgaagggaaa atctctgccg atatcatgac gtctgctgtc gtaataagtt cgtggtacac
     3061 agatgaatat aaaaaattat ttgcgttacc tgatgaatct gaactgcagc agaaggacgc
     3121 cgaggagctg ttcgactggc tcattgagga gtgccgcgga gaatgcccgc cccgtgtcag
     3181 gaaaaattat attctccagt gtggcccggg ccggttcaga aacaggaaaa aactgaacgc
     3241 attgctgaac attcttgaat ctcagttcag gctttctgtc gtgccggaag gaaaaaccat
     3301 gtatgttctg cttccccaga tagccagttt aaagttgtct gatgtctctg gtatttttac
     3361 ctcgggttat cactacaata agctgagagc gaagtaaccg gtgagcagtt tttctccatt
     3421 atgcttcaga cttagatgat tgattattat tgggatttca tcaccgatat tcagatgtat
     3481 gaatatcggt gatgaactaa agctcaggag ctgcgtggtg gtgatgaatc aacaggcata
     3541 taactgatac ccgccggtca gaagttccag cctgcgactt tcctgtctgc gtgcccgcag
     3601 tccggcgttc aggtgcagcg tgtgacagaa cgcacggaga gcgaaacggc aggcagacag
     3661 gccgttcagt cagccctggc accctgcccg gataccagct tccctgaaac aactggcatc
     3721 aggtgcgctt atcgtcaacc gttgtgcggt atgccaggag cacgtactga tccatcagat
     3781 ccgtcagtgc tgcgctctgc ttctgacggt tgatgatcca ttcctccagc tctttttctg
     3841 atttccgttt cagattctcc gtccagtcgt caaggcagtt tccggtcatt aaatcaagcc
     3901 agcaaagcca gaccagtttg aggcgcaact ccgtgccacc aaactgacgg aagaggctga
     3961 tcagtttatt tgccagcgaa cgctgcctca gacgttcaca ttcagacagg agaatgcctt
     4021 ccagcatcgg atgatgaacc agcagatgtt cactggtgca taaattcact atcagatcat
     4081 catccagggg cggcgtggtc cgtttgtccg gaccataaat cagccggtgc agactctggg
     4141 gtacgatggc aatctccggt ttttcaccat tgaatgacac ccacccgggc gtcagattat
     4201 ggaagtagta gatattcgga tgacacacgc cccaggatac ggcgtgccgg gtgaagccgg
     4261 tcagccattt ctggtagctt tcgtggagca gctcgtaacg ggtctgatac tgtggtttca
     4321 ttttcatatt cctcacgggg gataacaggc agcagaacgc cgccggcagg ccggagactg
     4381 cgtctgctgc agatgaacgg ttaagaatta cggacgggac tgtgcaatac gcgcctgcag
     4441 ccaggcttcc acttcgcttt tcagccatcg ggagctgcgg cccattttga tgggcgcagg
     4501 aaagtcacca tccttgatga gtttgtaaaa ccacttatcg gtcaggccgg tcagttgagt
     4561 gataaatgcc atgtcgacca tctggtcatc catcagtgaa actggggtga tcatgagaat
     4621 gttcctccgg atgttgttaa accagccggt gtggtcatga aaacaacgca ccaccttcac
     4681 cggcataaca accggggaga aacgtaaaaa ataatcggga atgtgaccgc caggagataa
     4741 acggggaggt tacggtggct gagccgtacc ggtatgccgg gtggttgtta cgaaacgatg
     4801 cttccgcacg cgcgcgcgat aaagaaggga tgattttcgt gaggaagagg ggaaaactgg
     4861 cgttctgata attgcagagt tatttacggg gcggttacga tggggcagac cgtgtccggt
     4921 gggcaggcga caggtatcac cgatgggata agactgtctg attggtatta taaatatata
     4981 agagagatca aacacatgag ctcaaataca tgagttcata tatgtgaaat aaacacggtg
     5041 agtgtgatca gtgggcatga tgagaacgca gtagcaacag actgcgctat ccctgatagc
     5101 tggcgtataa cctgcgtata taaaacgtac cagcaacgca cgtgaacaga gtgcattaag
     5161 catccgtcgt tgacaccgac ctggtgagtg acagtctgag agaccggtta agtggtggta
     5221 cgggagtaat gagtttgggg agggtaccag aatgtcagtc tggtacggta tgctgacagt
     5281 atcacgttaa ttgcagcata aagtgatgag aggctaatgg aagttaaaac cagagcagtt
     5341 ccgtcgttgt tcatcccggg agacactgga aaaagtgatt tcccacacgc gttataaact
     5401 tacccctgcg gagctggaaa ccttcaactc tgcggtcgat caccgactgg cagaactgac
     5461 aatgaacaag ctttacgatc gtgttcccgc ttcagtctgg aaatatgtca tctgagcgtt
     5521 cggtaatcac aactgcatcg cgccaggtgt tatccgcttt gtgattctta ttggctcttc
     5581 tcaggttatt aaaagctgaa tatgttcctg ttgtcgggaa ccaggataat accgggaacg
     5641 ttaagcggta tagcgacaac aaagaattcg aaatcaaaag ggaatggtgt ccggaggtgt
     5701 gggtgatcat ggg
//
LOCUS       DADTFG010000209         5708 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.210, whole genome shotgun sequence.
ACCESSION   DADTFG010000209 DADTFG010000000
VERSION     DADTFG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.210"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            82..1416
                     /locus_tag="J1138_004350"
     CDS             82..1416
                     /locus_tag="J1138_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA8456047.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVATLFALKIILVD"
     gene            1469..1921
                     /locus_tag="J1138_004351"
     CDS             1469..1921
                     /locus_tag="J1138_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBA8456048.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2132..3322
                     /gene="nepI"
                     /locus_tag="J1138_004352"
     CDS             2132..3322
                     /gene="nepI"
                     /locus_tag="J1138_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBA8456049.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVYGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYVLDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3363..3656)
                     /locus_tag="J1138_004353"
     CDS             complement(3363..3656)
                     /locus_tag="J1138_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456050.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3878..4696
                     /gene="nlpA"
                     /locus_tag="J1138_004354"
     CDS             3878..4696
                     /gene="nlpA"
                     /locus_tag="J1138_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBA8456051.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNTENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4700..5623)
                     /locus_tag="J1138_004355"
     CDS             complement(4700..5623)
                     /locus_tag="J1138_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA8456052.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
BASE COUNT         1347 a         1291 c         1482 g         1588 t
ORIGIN      
        1 aatgtgataa ttatcactga accgttgttg tcatttgttt tacaaaagca agggattaaa
       61 tctcctccaa tggacaaaaa aatgaataat gacaataccg attacgtgag taatgaatca
      121 gggacgcttt cgcgattatt taaactacct cagcatggga ccaccgtccg cacagaattg
      181 attgcgggga tgaccacttt tttaaccatg gtgtacatcg tttttgtgaa cccgcaaatc
      241 ctcggcgcgg cacaaatgga cccgaaagtg gtgtttgtta ccacctgttt gattgccggt
      301 atcggcagta ttgcgatggg ggtatttgct aacttacccg tggcactggc tccggcaatg
      361 ggcctgaacg ccttctttgc cttcgtggtc gtgggggcga tgggtatctc ctggcagacc
      421 gggatgggcg cgatattctg gggcgcagtt gggctatttt tgctcacgct gtttcgtatc
      481 cggtactgga tgatctccaa cattccatta agtttacgta ttggtatcac cagcggaatc
      541 ggattattta tcgccttaat gggattaaaa aatactggcg ttattgtcgc caataaagac
      601 accctggtga tgattggtga tttaagttct cacggcgtgt tgttaggtat tttagggttt
      661 tttattataa ctgtgttgtc atcacgtcat tttcatgccg cggtgcttgt ttctattgtg
      721 gtgacgtctt gctgtggatt atttttcggt gatgttcatt ttagcggcgt ctattccatt
      781 ccgcctgata ttagcggcgt cattggtgaa gtggatttga gcggtgcgtt atcacttgaa
      841 ctcgccggta tcattttctc ctttatgctg atcaacttat ttgattcatc agggacatta
      901 attggtgtga ctgataaagc tggcttaata gatagtaacg gtaaattccc caatatgaat
      961 aaggcgctgt atgttgatag cgtcagttcg gtggcgggtg cgtttatcgg tacctcgtct
     1021 gttaccgcct atattgaaag tacttcaggt gtggctgtcg gcggtcgcac ggggctgact
     1081 gcggttgttg ttggcgttat gttcctgctg gttatgttct tctcaccgct ggtggcgatg
     1141 gttcctcctt acgcaaccgc cggagcgtta atctttgttg gcgtattgat gacatcgagc
     1201 ctggcacgcg ttaactggga tgattttacc gaatcggtgc ctgcgtttat taccacggtg
     1261 atgatgccct ttacgttctc gatcaccgaa gggattgcac taggctttat gtcgtactgc
     1321 atcatgaaag tgtgcaccgg gcgctggcgc gatctgaacc tgtgtgtggt ggtggtcgca
     1381 actctgtttg cactgaagat tattctggtg gattagtaac tggcgagggt ttccagatat
     1441 catgagttct gattacgcag gagaactcat gatctggata atgctcgcca cgctggcggt
     1501 agtgtttgtg gttggttttc gggtgctgac atccggggcc agaaaagcga ttcgccgtct
     1561 cagcgatcgg ctgaacatcg atgtcgtacc cgtggagtcg atggtcgatc aaatgggaaa
     1621 gtcagcaggt gacgaatttt tacgttattt gcatcgcccg gatgagtcgc acctgcaaaa
     1681 cgccgcgcag gtgttgctca tctggcaaat tgtcattgtc gatggtagcg aacagaacct
     1741 gctgcaatgg catcggattt tacaaaaagc ccgccttgcc gcgccgatta ccgatgctca
     1801 ggtcaggctg gcgctgggtt ttctgcgcga aaccgaacct gaaatgcagg atatcaatgc
     1861 ttttcagatg cgctataacg cgttctttca gcctgccgag ggcgttcact ggctgcatta
     1921 aaaccgcttc gtcttatcag cctacagacc gcgcctgacc tggtgggtgg ataagacgtt
     1981 cacaccgcat ccggtaatct ttgccctgtt ccgtttcgat cttaactcca gctcaagcaa
     2041 caccgtaaaa gttgcaaaaa cgttaaatac gtcacacatt tcaatgttaa agtggtcggc
     2101 tttccctctg aaacatgcca cgggtaacac catgagtgaa tttattgctg aaaaccgcgg
     2161 cgcggatgcc atcacccgac cgaactggtc agccgttttc tcggtggcgt tttgcgtcgc
     2221 ctgtctgatt atcgttgagt ttttgcctgt cagtttgttg acgccaatgg cccaggattt
     2281 aggcatttcg gaaggggttg ccgggcaatc ggtgaccgtg accgcctttg tggcgatgtt
     2341 tgccagtttg tttattaccc agactattca ggccactgac cgccgctacg ttgtcattct
     2401 gttcgccgtt ttgctgacgc tttcctgttt gctggtttcc tttgccaact cattcagtct
     2461 gctgttaatc ggtcgcgcct gtctggggct ggcgctgggc gggttctggg cgatgtcggc
     2521 gtcgctgacc atgcgtctgg taccgccgcg tacggtgccg aaggcgctgt cggtgatctt
     2581 cggcgcggtt tctattgcgc tggtgattgc cgcgccgttg ggcagttttt taggcgagct
     2641 tatcggttgg cgcaatgtct ttaatgcggc ggcggcgatg ggcgtgctgt gtattttctg
     2701 gattatcaaa tcactgcctt cgctgccagg tgaaccttcg catcagaaac aaaatacttt
     2761 ccgcttatta caacgtccgg gagtgatggc cgggatgatc gccatcttta tgtcctttgc
     2821 cggtcagttt gcttttttca cttatattcg cccagtgtat atgaatctgg cgggattcgg
     2881 tgtttatggc ttaaccctgg tgctgctgag ttttggtatc gccagctttg tcgggacatc
     2941 gctttcgtcg tttattctca aacgttcggt gaaactggcc ttagcgggcg caccgtttgt
     3001 gctcgccctg agcgcgctgg tactgacgct gtggggaagc gataaaattg ttgctactgg
     3061 tgtggcgatt atctgggggc tgacctttgc tttaattccc gttggttggt caacgtggat
     3121 cactcgctca ttggccgatc aggcagaaaa ggctggatct attcaggtgg ccgttattca
     3181 gcttgctaac acctgcggtg cggcaatcgg cggttatgtg ttggataaca ttggtctgac
     3241 ttcgccgctg atgttgtccg gcacattgat gttgctgact gcattgttgg tgactgcaaa
     3301 ggtgaaaatg aagaaatcct gatatcaggt aaagaagcgt tggtcagtgg ttgttgccgt
     3361 tcttacatat ccgggcattc taccttaccc aacgcttccc aataattgct ctgattatta
     3421 cgttccatcg ccacgatggc ctcgcgtata tgttgtttat tgttttcatc tgacaacatt
     3481 aacttttccc acgcttcatc tgataatgac gcctgtggcg tgcaaatttt acgtaaatct
     3541 tgcaataccg tggttttctc ttttgccgac tgtagagaac tgaatggcga ctcggcctga
     3601 gaaatggctg gaaaaatcag gaatacggta atcatgagta gcatcgttgg cttcatagcg
     3661 gcaaccttct gcaaatgtgc atcgaatgaa gcataagtgt agtttgcttt tgccgctctc
     3721 atcatgctgt acgaaaggtg tttgcgtggt cagtaagaag tgccagactt tatattccac
     3781 ttttattcct ttttattctt atcgatagcg tttcgttttt taaactgcag cgaccttacc
     3841 gctatagtca ggtaatcatt aataaaagga taaaaaaatg aaactgacaa cacatcatct
     3901 acgggcgggg gccgcattat tactggcagg agttctgctg gcaggttgcg accagagcag
     3961 cagcgatgaa aagcatatta aagttggcgt tattaatggc gcagaacaag atgtcgcgga
     4021 agtcgctaaa aaggtagcaa aagagaaata tggcctcgat gttgaactgg taggatttag
     4081 cggttcgttg ctaccgaacg atgcgacaaa tcatggcgag cttgatgcga acgtcttcca
     4141 gcatcgaccc tttcttgaac aagataatca ggcgcatggt tataaactgg tagcggtggg
     4201 aaatacgttt gtcttcccca tggcgggcta ttccaaaaaa atcaaaacag tggcgcaaat
     4261 aaaagaaggt gcgacagtgg cgatccccaa cgacccgact aaccttggtc gcgcgctttt
     4321 actgctgcaa aaagagaagc taattactct gaaagaggga aaagggttat tacctaccgc
     4381 gttggatata accgataatc cacgccattt gcagataatg gagctggaag gggcacagtt
     4441 gccacgtgta ctggacgacc cgaaagtgga tgtagcgatt atcagcacca cttacatcca
     4501 gcaaacagga ctttctccgg ttaacgacag cgtatttatt gaagataaaa attcgccgta
     4561 tgtgaatatt ctggtggcac gggaagataa taagaataca gaaaacgtga aggaatttct
     4621 gcaatcttat caatcacccg aagtcgctaa agcggcagaa accatcttta acggtggcgc
     4681 ggtgcctggc tggtaattct cactcatgcc gcgccggacg atttattttt ctggcgcggc
     4741 ggcgggaatc cattgaaatc aaaatcaccg atgacagaat cagcagcgtt cccagccagt
     4801 ccggcaatgt gaaggtgatt cccagcaaca gcaaagagag cagcgcgcta cttaatggtt
     4861 ctgcacagct taaaatgctg gcttttggac cgccaattaa ttgtgctcct ttcaggtaca
     4921 gactaaatgt cagggacgta ccaatgacca ccaaataaaa aaacgccaga atcaaactgc
     4981 cgttaacgac aaagtttgtc ccttgtctgg cataaaaggg cagcaggatc agaccgccaa
     5041 tcagcatact ccagccgacg accggtaacg tgccatagcg ggcaatcagc gttgagggat
     5101 aggtggtata gaatgcagca gcaaacgccg aggcaatgcc ccagaataac gcggcaggag
     5161 agatcgataa tgacgtcgga ttaccgtgtg tcaccaataa aaaagtcccg atcagcgatg
     5221 tcaaaatagc gcagaaaacc agaatgcccg ggcgggattt acgcaccagt gagaaccagg
     5281 cgacgataat agtcggtgag aggaattgca gcaccgttgc cgtggctgcg ttcgattttt
     5341 cgatggttag caaaaaagtg agctgtacgg ttagcgcgcc aaccacggaa aaaatcagta
     5401 ggctaatggc atctttgtga ttgttaataa tagaaaagat tttatcacca tgaacaaatg
     5461 atagcgtcag cagaatcaaa ccggcgaata tcaaacgcgt catagtcaaa aactgcgacg
     5521 acatctggct ttgctccatg atgtattgcg cgcagacccc tgaacttccc cacaacacgg
     5581 cggcaatcag aacgttcagc atcccctttc tggtggaacc catttttccc tctgcatact
     5641 gttttgtctt ttcatcaata tacgttaagg ataccactac cgatgacggt gagggagtag
     5701 agtaatca
//
LOCUS       DADTFG010000210         5619 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.211, whole genome shotgun sequence.
ACCESSION   DADTFG010000210 DADTFG010000000
VERSION     DADTFG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.211"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            94..435
                     /locus_tag="J1138_004356"
     CDS             94..435
                     /locus_tag="J1138_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456053.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIDPIAFFVC
                     LFGQMRLFRSA"
     gene            481..918
                     /locus_tag="J1138_004357"
     CDS             481..918
                     /locus_tag="J1138_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBA8456054.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(960..1955)
                     /locus_tag="J1138_004358"
     CDS             complement(960..1955)
                     /locus_tag="J1138_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBA8456055.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGVIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAVSLLLSMLVQRIDRNRLVS"
     gene            2130..2429
                     /locus_tag="J1138_004359"
     CDS             2130..2429
                     /locus_tag="J1138_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456056.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2524..3435
                     /gene="glyQ"
                     /locus_tag="J1138_004360"
     CDS             2524..3435
                     /gene="glyQ"
                     /locus_tag="J1138_004360"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBA8456057.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3445..5514
                     /gene="glyS"
                     /locus_tag="J1138_004361"
     CDS             3445..5514
                     /gene="glyS"
                     /locus_tag="J1138_004361"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBA8456058.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         1429 a         1362 c         1440 g         1388 t
ORIGIN      
        1 aatttattct gaaaatcatt tgttaatggc atttttcagt tttgtctttc gttggttact
       61 cgtaatgtat cgctggtaga tatggagatc gttatgaaaa cctcaaagac tgtggcaaaa
      121 ctattatttg ttgtcggggc gctggtttat ctggttgggc tatggatctc atgcccattg
      181 ttaagtggaa aaggttattt tcttggcgtg ttaatgacgg caacttttgg caactatgca
      241 tatcttcgcg cagaaaaact tgggcaactg gatgattttt ttatccacgt ctgtcagttg
      301 gtagcgttaa tcactatcgg tctcttgttt atcggtgttt taaacgcacc tatcaatgct
      361 tatgaaatgg tgatcgaccc catcgccttt tttgtctgct tgtttggtca aatgcgtttg
      421 tttcgctcgg catgagcaac ataaagttct tacacattca ggaatgaaag gaatactgtg
      481 atggacaaca aaatatcaac ctattctccg gcatttagta ttgtttcatg ggtagccctt
      541 attggcggta ttgttaccta tctgttagga ctatggaatg cagagatgca gttaaatgaa
      601 aaagggtatt attttgccgt actggtatta ggtttgtttt ccgcggcgtc ttatcaaaag
      661 accgttcggg acaagtatga aggcatacct accaccccca tttattatat gacttgcctg
      721 acggtcttta ttatctctgt tgcgttgttg ctggtaggtc tatggaatgc aacgttatta
      781 ctcagcgaaa aaggctttta tgggctggct ttcttcttaa gcttgtttgg tgcagtagcg
      841 gtgcagaaga atattcgtga tgccggaata aacccaccaa aagaaacaca ggttacccag
      901 gaagaataca gcgaataatt cacataagcc cggtcagtcc aatgtgaccg ggcttctact
      961 taactcacta atctgtttct gtcgattcgt tgtaccagca tagaaagtaa taaactcact
     1021 gccagggtcg cgcaaaagat ccaaataata tcgagtattg gccagttttt aagctcaatc
     1081 ccccgggtgc gcagtgcatg gataatcaat gcatggaacc cgtatatacc taacgaatgg
     1141 cgggagatta agccaagtcc gcaaatagta cgcgtataca gcgtgttttt aaccagagtc
     1201 aataacgcga ttgcgcagat aaaaaccatc ggcccacagt aaagatacca ggtatcggca
     1261 aaatttccgc gccactgtaa ttcatataat gtcccgcgag agataataaa aacccccgtc
     1321 gcaaacagtg cggcgctcac ccacgacagt gctttatgct gtgtgtccat catccctata
     1381 gcgcggccca acatgccata aaggatgtag taaaaagtat cgccattgat atataagtta
     1441 attggcagcc attcaaaacc gtcgattttc tgcggcacgg tgtttgggtt agcgatgaca
     1501 ccaatcacca ccattagcac cagcaacatt tttccgccga cgttcttcac ctgaatcagc
     1561 ggtgaaacca gataaatcac tgcaatcgcg aagaaaaacc ataaatggta aaacactggc
     1621 ttttgcagca ggtttttcag cgctaactcc acattgatgg aggtaaacag cgcaatgtag
     1681 agcagcgcga ttgcgctata aaaaaacagg cataagccga tacgcaagaa atggcgcgac
     1741 tgggcgctgc gttcgccaaa aaagagatag ccggaaatca tgaaaaatag cggcacgctg
     1801 acacgagagg cagaattcag aacattggcg atatcccagg tgatggggct aacactatga
     1861 gcattggtca cataccaggt agtggtgtga atcatcacca ccattaaaca cgctatccct
     1921 cgcaggttat caatccagta aattttgggc tgcatctgtg tctctgtatc tggttaaaaa
     1981 aagtctgacc gataaatcat tcagttggcg cactggaata atctgagttt tatcactaca
     2041 gcttatagag gcttaaggaa attcgtaaga tatcagccac tataccgata taaataataa
     2101 gactcacctg caaaccagac ggtaatttaa tgatgatgaa cgctttcttt ccagcaatgg
     2161 cgcttattgt gctagtgggt tgttctacac cgccacccgt gcagaaagca caacgggtaa
     2221 aggttgatcc tctgcgttcg ttgaatatgg aagcgttatg caaggatcag gcggcaaaac
     2281 gttataacac cggcgagcaa aaaatcgacg tcaccgcctt cgaacagttc cagggaagct
     2341 acgaaatgcg cggttatacc ttccgtaaag agcagtttgt ctgttctttt gacgcagatg
     2401 gccatttttt gcatctttcc atgcgttaag ccctgctttt tcccgtttcg tactgtatat
     2461 cttccatcca gcgggtatac tgatcccttc ctttaaatcc acacgtatcc agcacgaaat
     2521 aatatgcaaa agtttgatac caggaccttc cagggcttga tcctgacctt acaggattac
     2581 tgggctcgcc agggctgcac cattgttcaa ccattggaca tggaagtcgg cgcgggaacc
     2641 tctcacccaa tgacctgtct gcgcgcgctg gggccagaac cgatggcggc tgcttatgtt
     2701 cagccttctc gtcgcccgac cgatggtcgc tacggcgaaa accccaaccg tttacagcac
     2761 tactatcagt tccaggtggt cattaagcca tcgccggaca atattcagga gctgtacctt
     2821 ggttctctga aagagctggg catggacccg accatccacg acatccgttt cgtggaagat
     2881 aactgggaaa acccgacgct gggtgcctgg gggctgggct gggaagtgtg gctgaacggc
     2941 atggaagtga cgcagttcac ttacttccag caggttggtg gtctggagtg taaaccggtt
     3001 accggcgaga tcacctatgg tctggaacgt ctggcaatgt acattcaggg cgtagacagc
     3061 gtttacgacc tggtctggag cgacggcccg ctgggtaaaa ccacctacgg cgacgtgttc
     3121 catcagaacg aagtggagca gtccacttac aacttcgaat acgcggatgt ggacttcctg
     3181 ttcacctgct ttgagcagta cgagaaagaa gcgcagcaac tgctggcgct ggaaaatccg
     3241 ctgccgctgc cagcctacga gcgtattctg aaagccgccc acagcttcaa cctgctggat
     3301 gcgcgtaaag ccatctccgt caccgagcgt cagcgctata ttctgcgcat tcgcaccctg
     3361 accaaagcag tggcagaagc atactacgct tcccgtgaag ccctcggctt cccgatgtgc
     3421 aacaaagata agtaagaggc ggctatgtct gagaaaactt ttctggtgga aatcggcact
     3481 gaagagctgc caccaaaagc actgcgcagc ctggctgagt cctttgctgc gaactttact
     3541 gcggagctgg ataacgctgg cctcgcacac ggcaccgttc aatggtttgc tgctccgcgt
     3601 cgtctggcgc tgaaagtcgc taacctggcg gaggcgcaac cggatcgcga aatagaaaaa
     3661 cgcggcccgg caattgccca ggcgttcgac gctgaaggca aaccgagcaa agcggcagaa
     3721 ggttgggcgc gtggttgcgg tattaccgtt gaccaggctg agcgtctgac taccgataaa
     3781 ggcgaatggc tgctgtatcg cgcccatgtg aagggtgaaa gcaccgaagc actgctgccg
     3841 aatatggttg cgacttcgct ggcgaaactg ccgatcccga aactgatgcg ttggggcgca
     3901 agcgacgtgc acttcgtgcg tccggttcac accgtgaccc tgctgctggg cgacaaagtt
     3961 attccggcta ccattctggg tattcagtcc gatcgcgtga ttcgcggcca ccgctttatg
     4021 ggcgagccgg aattcactat cgacaatgcc gatcagtatc cggaaattct gcgtgagcgc
     4081 gggaaagtca tcgccgatta cgaagaacgt aaggcgaaga ttaaagccga tgctgaagaa
     4141 gcagcacgta agattggcgg taacgctgat ttaagcgaaa gcctgctgga agaagtggct
     4201 tcgctggtgg agtggccggt cgttctgacc gcgaaattcg aagagaaatt cctcgcggtg
     4261 ccgtctgaag cactggttta caccatgaaa ggtgaccaga agtacttccc ggtgtatgcg
     4321 aacgacggca aactgctgcc gaactttatc ttcgttgcca acatcgaatc gaaagatccg
     4381 cagcagatta tctccggtaa cgagaaagtc gttcgtccac gtctggcgga tgccgagttc
     4441 ttcttcaata ccgaccgtaa aaaacgtctt gaagataacc tgccgcgcct gcaaaccgtg
     4501 ttgttccagc aacagttggg gacgctgcgc gacaaaactg accgcattca ggcgctggct
     4561 ggctggattg ctgaacagat tggcgctgac gttaaccacg ctacccgtgc gggcctgctg
     4621 tctaagtgtg acctgatgac caacatggtc ttcgagttca ccgacaccca gggcgttatg
     4681 gggatgcact acgcgcgtca cgatggcgaa gcggaagacg tagctgtcgc gctgaacgaa
     4741 cagtatcagc cgcgctttgc cggtgatgac ctgccgtcta acccggtagc ctgtgcgctg
     4801 gcgattgctg acaagatgga taccctggcg ggtatcttcg gtatcggcca gcatccgaaa
     4861 ggcgacaaag acccgtttgc gctgcgtcgt gccgcacttg gcgtgctgcg tattatcgtt
     4921 gagaagaacc tcaaccttga tctgcaaacg ctgaccgaag aagcagtgcg tctgtatggc
     4981 gataagctga ctaacgccaa cgtggttgat gatgttatcg actttatgct cggtcgcttc
     5041 cgcgcctggt atcaggacga aggttacacc gttgacacca tccaggcggt actggcgcgt
     5101 cgtccgactc gtccggctga tttcgatgcc cgtatgaaag cggtatcgca cttccgtacc
     5161 ctggaagcag ctgcagcact ggcggcggcg aacaagcgtg tatctaacat cctggcgaaa
     5221 tctgatgaag tgctgagcga tcgcgtgaat gcctctactc tgaaagagcc ggaagaaatt
     5281 aaactggcga tgcaggttgt ggtgctacgt gacaagctgg agccgtactt tgcggaaggt
     5341 cgttaccagg atgcgctggt cgaactggct gagctgcgtg aaccggtcga tgctttcttc
     5401 gataaagtga tggtcatggt tgatgataaa gaattgcgta tcaaccgtct gaccatgctg
     5461 gaaaaactgc gcgaactgtt cctgcgcgtt gcggatattt cgctgttgca gtaataacgc
     5521 cgttattaaa aagcctgcca tctggcaggc tttttttatc gctaaataat acagcaacct
     5581 ttaataatct tctgctgaat aaagattgtc tcatatatt
//
LOCUS       DADTFG010000211         5540 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.212, whole genome shotgun sequence.
ACCESSION   DADTFG010000211 DADTFG010000000
VERSION     DADTFG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.212"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            146..1480
                     /locus_tag="J1138_004362"
     CDS             146..1480
                     /locus_tag="J1138_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8456059.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1496..2266
                     /gene="garL"
                     /locus_tag="J1138_004363"
     CDS             1496..2266
                     /gene="garL"
                     /locus_tag="J1138_004363"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBA8456060.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2296..3186
                     /gene="garR"
                     /locus_tag="J1138_004364"
     CDS             2296..3186
                     /gene="garR"
                     /locus_tag="J1138_004364"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBA8456061.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGM
                     ADHSALACYYEKLAKVEVTR"
     gene            3283..4428
                     /gene="garK"
                     /locus_tag="J1138_004365"
     CDS             3283..4428
                     /gene="garK"
                     /locus_tag="J1138_004365"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBA8456062.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4461..4837
                     /gene="rnpB"
                     /locus_tag="J1138_004366"
     ncRNA           4461..4837
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="J1138_004366"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1342 a         1290 c         1519 g         1389 t
ORIGIN      
        1 taaattagca tttgcacaat gattattatt ctcgcgctgc ttatactaat tcccgagttc
       61 attgtcgtaa cggcataaat gaaactcata aataacacct tccccataat aataaaaatc
      121 agcataagta cccgaggtaa ataaaatgat gctggacacc gttgacgaaa aaaagaaagg
      181 cgtgcatacc cgctatttaa tattactgat tatttttatt gttaccgccg ttaactacgc
      241 cgatcgtgca acactgtcta ttgctggtac cgaagtggca aaagagttgc agttaagtgc
      301 ggtttcgatg ggttacatct tctctgcttt tggctgggcc tacttgctga tgcaaattcc
      361 cggcggctgg ctgcttgata agtttggctc gaagaaagtt tacacttaca gcctcttttt
      421 ctggtcgctg ttcaccttcc tgcaaggctt tgtcgatatg tttccgctgg cctgggctgg
      481 gatctccatg ttctttatgc gatttatgct ggggttctcg gaagcgccat cattcccggc
      541 gaacgcccgt attgtcgccg cctggttccc gacgaaagaa cgtggtactg cctccgccat
      601 ctttaactcg gcgcaatatt tctcgttggc gctcttttcg ccgctgcttg gctggctgac
      661 tttcgcctgg ggctgggagc acgtctttac cgttatgggg gtgattggtt ttgtgctgac
      721 ggcgctgtgg atcaagttga ttcataatcc gacagatcac ccacgtatgt ctgcggaaga
      781 gctgaagttt atctcggaaa atggcgcggt ggtcgatatg gaccacaaaa agccgggcag
      841 tgcggcagca agcggaccca aactgcatta catcaagcaa ttgctctcta accgcatgat
      901 gctgggcgta tttttcggac aatattttat caacaccatc acctggttct tcctcacctg
      961 gttcccaatt tatctggtgc aggaaaaagg catgtcgatt ctgaaagtgg gtctggtcgc
     1021 ctcgattccg gcactgtgtg gctttgcggg cggcgtgctg ggaggtgtct tctcggatta
     1081 tctgatcaaa cgcggtttgt ccctgaccct ggctcgtaag ctaccaattg tgctgggaat
     1141 gttgctggct tccaccatca tcttatgtaa ctacaccaac aacaccacgc tggtggtcat
     1201 gctgatggcg ctggctttct ttggtaaagg atttggtgcg ctgggctggc cagtgatttc
     1261 tgacaccgcg ccgaaagaga ttgttggcct ctgcggcggc gtctttaacg tctttggcaa
     1321 tgttgcctcc attgtcactc cactggtgat tggctacctg gtaagtgaac tgcactcctt
     1381 caatgcagca ctggtttttg tgggatgttc agcgctgatg gcgatggtct gctacctctt
     1441 cgtagtcggc gacattaaac gtatggaatt gcagaaataa gcaaaggtat aagcgatgaa
     1501 taacgatgtt ttcccgaata aattcaaagc cgcactggct gcgaaacagg tacaaattgg
     1561 ttgctggtca gctctctcta acccgattag cactgaagtt cttggtttgg ctgggtttga
     1621 ctggctggtg ctggatggtg aacatgcgcc aaacgatatc tccacgttta ttccgcagtt
     1681 aatggccctg aaaggcagcg ccagcgcgcc agtagtgcga gtgccgacca acgagccggt
     1741 aattattaag cgtcttctgg atatcggttt ctataacttc ctgattcctt ttgtagaaac
     1801 aaaagaggaa gcagagcagg cggtggcatc aactcgttac ccaccggaag gcattcgcgg
     1861 cgtctccgtt tctcaccgcg ccaatatgtt tggcaccgtg gcggattatt tcgctcagtc
     1921 gaacaagaac atcactattc tggtccagat agaaagtcag cagggcgtag ataacgtcga
     1981 tgctattgcc gctaccgaag gtgtagacgg catcttcgtc ggccccagcg atctggctgc
     2041 ggcattaggc catctcggca atgcctcgca tccggatgta caaaaagcaa ttcagcacat
     2101 ttttaaccgt gccagcgcgc acggcaaacc cagcggcatc ctcgcaccag tcgaggctga
     2161 tgcgcgtcgt tatctggaat ggggcgcgac gtttgtggcc gtcggcagcg atctcggcgt
     2221 cttccgctct gccactcaga aactggctga tacctttaaa aaataaccac caccgcaata
     2281 agagagatga ttgatatgac tatgaaagtt ggttttattg gccttgggat tatgggtaaa
     2341 ccaatgagta aaaacctttt gaaagcaggt tactcgctgg tggttgctga ccgtaaccca
     2401 gaagctatcg ctgacgtgat tgctgccggt gcagaaacag cctctacggc taaagcgatc
     2461 gctgaacagt gcgacgtcat catcaccatg ctgccaaact cccctcatgt gaaagaggtg
     2521 gcgctgggtg agaatggcat tattgaaggc gcgaagccag gtacggtatt gatcgatatg
     2581 agttcgatcg caccgctggc aagccgtgaa atcagcgaag cgctgaaagc gaaaggcatt
     2641 gatatgctgg acgccccggt gagcggcggt gaaccgaaag ccatcgacgg tacgctgtca
     2701 gtgatggtgg gcggcgacaa ggctattttc gacaaatact atgatttgat gaaagcgatg
     2761 gcgggttccg tggtgcatac cggggaaatc ggtgcaggta acgtcaccaa actggcaaat
     2821 caggtcattg tggcgctgaa tattgctgcg atgtcagaag cgttaacgct ggcaaccaaa
     2881 gcgggcgtta acccggatct ggtgtatcag gcaattcgcg gtgggctggc gggcagtacc
     2941 gtgctggatg ccaaagcgcc gatggtgatg gaccgcaact tcaaaccggg cttccgtatt
     3001 gatctgcata ttaaggatct ggcgaatgcg ctggatactt ctcacggcgt cggcgcgcaa
     3061 ctgccgctca cagctgcggt tatggagatg atgcaggcac tgcgtgcaga tggtttagga
     3121 atggcggatc atagcgccct ggcgtgctac tacgaaaaac tggcgaaagt cgaagttact
     3181 cgctaatgac gtcgtgcccg gatggcgtat tgcaatccgg gcctggaatc caggccgaat
     3241 aagagaaacg ccctccggcg tcatgtggta acaggcatag gtatgaaaat cgtaatcgcc
     3301 ccagactctt ataaagaaag tttatctgcc agcgaggttg cgcaggcgat agaaaaagga
     3361 tttcgggaaa tttttcctga tgcacagtat gtttctgttc cggttgccga cggtggtgaa
     3421 ggaacggtgg aagcgatgat tgcagccacc cagggggctg aacgtcacgc ctgggttaca
     3481 gggccgctgg gcgagaaagt gaatgccagt tgggggatct ccggcgatgg caaaaccgcg
     3541 tttatcgaaa tggcggcggc cagtgggctg gaactggtac ctgcggaaaa acgcgatcca
     3601 ctcgtgacca cttcacgcgg tactggtgag ttaatcctgc aggcgctgga gagcggtgcg
     3661 acaaacatta ttatcggcat tggcggcagc gccaccaacg acggcggcgc aggcatggta
     3721 caggcgctgg gggcgaaatt atgcgatgcc aacggcaatg aaattggttt tggcggcggt
     3781 agtcttaata ctctgaatga tattgatatt tccggtctcg atccgcgctt aaaagattgt
     3841 gtcatccgcg tcgcttgtga tgtcaccaac ccgttagtcg gcgacagtgg tgcttcgcgc
     3901 atctttggcc cacaaaaggg agccagtgag gcgatgattg tcgagctgga taataacctc
     3961 tctcactatg ccgatgtcat taaaaaagcg ctgcatgttg atgtgaaaga tgtccccggc
     4021 gcaggagctg cgggcggtat gggcgcggcg ctgatggcgt ttcttggtgc agaactgaaa
     4081 agtggtattg aaatcgtcac tacggcgctg aatctggagg aacatattca cgattgtacg
     4141 ctggtgatca ctggtgaagg gcgtattgat agccagagta ttcacggcaa ggtaccgatt
     4201 ggtgtcgcaa acgtggcgaa gaagtaccat aaaccggtga ttggcattgc gggtagcctg
     4261 accaatgatg ttggcgttgt acatcagcat ggcattgatg cggtcttcag cgtactgacc
     4321 agcattggta cgttagacga agcattccgc ggagcttatg acaatatctg ccgtgcttca
     4381 cgtaatatcg ccgcgacact ggcgattgga atgcgcaacg cggggtgaca agggcgcgca
     4441 aaccctctat actgcgcgcc gaagctgacc agacagtcgc cgcttcgtcg tcgtcctctt
     4501 cgggggagac gggcggaggg gaggaaagtc cgggctccat agggcagggt gccaggtaac
     4561 gcctgggggg gaaacccacg accagtgcaa cagagagcaa accgccgatg gcccgcgtaa
     4621 gcgggatcag gtaagggtga aagggtgcgg taagagcgca ccgcgcggct ggtaacagtc
     4681 cgtggcacgg taaactccac ccggagcaag gccaaatagg ggttcataag gtacggcccg
     4741 tactgaaccc gggtaggctg cttgagccag tgagcgattg ctggcctaga tgaatgactg
     4801 tccacgacag aacccggctt atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg
     4861 gcgggttttt gcttttggtg cacagggtaa gatgaatatc tacggtatta ataacagact
     4921 ttgtaagaag ttattaagtc tatattgcat ttaaaaggtc gtaattatac atatatttct
     4981 ctcattaatt gtaagttcgc aggtattgtc cgatcgttga atcatttggg cgttataacc
     5041 taaattttta ccgtccaata atcaatattt ctattgtgat tgttaaccac tgcgtgcatt
     5101 gacgggattg aagggtgaaa aatatgtgca attgagaagc ctttcattga tgccagcctt
     5161 gtcatcatga caaacaataa tagatgctta ttttcctcgc agggttcttt cgcactatca
     5221 aaaaatgctc atttacccgg cattacagtc aacgaagcaa cattctatgg cctacgctga
     5281 tgtaaagcac agcgaaaatc ctccttgttg acttcgtctg accttatcgc ggcgaccagc
     5341 aattattcgt tttgtgatcc tcaacacaaa atgctattcg ttgcctatga ggggagaaga
     5401 atttatggaa aacgattgca ttcacaaaat gacgtcctgt cagaagagaa aaagttgacg
     5461 ttaatcaatt gttcgagatg atcaatacct gtattcgaaa ttttaaaata ttgataaaat
     5521 aaatgtttgt tctccaacaa
//
LOCUS       DADTFG010000212         5515 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.213, whole genome shotgun sequence.
ACCESSION   DADTFG010000212 DADTFG010000000
VERSION     DADTFG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.213"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            224..1438
                     /locus_tag="J1138_004367"
     CDS             224..1438
                     /locus_tag="J1138_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBA8456063.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1505..2788
                     /locus_tag="J1138_004368"
     CDS             1505..2788
                     /locus_tag="J1138_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8456064.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVSGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3040..3957
                     /gene="oxyR"
                     /locus_tag="J1138_004369"
     CDS             3040..3957
                     /gene="oxyR"
                     /locus_tag="J1138_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBA8456065.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3940..5340)
                     /gene="sthA"
                     /locus_tag="J1138_004370"
     CDS             complement(3940..5340)
                     /gene="sthA"
                     /locus_tag="J1138_004370"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBA8456066.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1237 a         1260 c         1505 g         1513 t
ORIGIN      
        1 ttcattttta atgattaatg gagtttgtta tgtgattatt taacgtgttg ttttaacgat
       61 gatggctaat tattcaggtg gtttttgagg gtgttttttc tcctcggtag aaatttttat
      121 ttttaatgtg atcacggttg aataatcact ttttctctct tgtatggtag taggcgtaaa
      181 attaagtttg aagtgtctat ttacaataca ggagattgca ataatgaaaa ttattgctgt
      241 ggatgttttt gttacttgtc ccgggcgtaa tttcgttacg cttaaaataa ccactgaaag
      301 cggactttgt ggattaggag atgccacgtt aaatggtcgc gaattgtcgg tcgcttctta
      361 tctgaaagat catctttgtc ctcaattaat tggccgcgat gccagccgta tcgaagatat
      421 ctggcaattc ttttataaag gagcttactg gcgacgtggt cccgtcacga tgtcggccat
      481 ttctgccatt gatatggcgt tgtgggatat caaagctaaa gcggcaaata tgccattgta
      541 tcaactgctt ggtggcgctt ctcgtgaagg cgtgatggtt tattgccaca ccaccgggcg
      601 gacgattgat gaagtccttg aagactatgc aaagcaccag cagatgggct ttaaggcgat
      661 tcgtgtgcag tgtggtgtac cgggtatgca aaccacctat ggtctggcga agggcaaagg
      721 actggcgtat gaaccagcaa ccaaaggcct gtggccagaa gagcagttgt ggtcttcaga
      781 aaagtatctc gattttacgc cgaagttatt tgaagcggtg cgtaataagt tcggttttaa
      841 cgaacatctg ttgcatgaca tgcaccatcg cctgaccccc atcgaagcgg cgcgctttgg
      901 gaggagcatt gaggattacc gcctgttctg gatggaagat ccgacgcctg ccgagaacca
      961 ggaatgtttt cgtctgattc gccagcatac cgtgacgccg attgcggtcg gtgaagtgtt
     1021 taacagtatc tgggactgca aacagttaat cgaagagcag ctgattgact atattcgcac
     1081 gacgattacc catgcaggcg gcattaccgg gatgcgacgg attgccgatt ttgcatcgct
     1141 ttatcaggta cgtaccggtt cgcatggccc ttcagattta tcgcctgttt gtcatgcggc
     1201 ggcgctgcat tttgacctct gggtgccaaa ctttggcgtt caggagtaca tgggctattc
     1261 cgaacaaatg cttgaagtct tcccgcacag ctggcgcttc gatgaaggtt atatgcaccc
     1321 aggcgatgaa ccggggctgg gtatttcttt cgatgagaaa ctggcggcga aatatccgta
     1381 tgacccggcg tatctccccg tcgcacgact ggaagatggc acattgtgga actggtaata
     1441 gcggttcgct taattatttt ctgaatattc atcaaatcga ttaattccgc tcaaggagct
     1501 aaaaatggac gttgatgtgt caaccagtgt tgcgggtaat aaaccgcaac ggattcgtcg
     1561 tattcagaca gtgaccctgg ttttattatt tatggcgggg atcgttaatt ttctcgaccg
     1621 ttcatcattg agcgtggcag gtgaagcgat tcgtggcgag ttaggattat cggcgacaga
     1681 gtttggtgtt ttgctttctg cattttctct gtcttatggt ttttcacaac ttccttccgg
     1741 tattttgcta gaccgctttg gcccacgaat agtgttaggc gcaggtttaa tattctggtc
     1801 attaatgcag gcattaacag gaatggttaa ttcttttagc cactttattt taatgcgtat
     1861 cggtctgggt attggcgaag cgccatttat gcctgcggga gtaaagtcga tcaccgactg
     1921 gtatgcgcaa aaagaacgcg gcacggcgct ggggattttt aactcatcta ccgttagcgg
     1981 tcaggctatc gcgcctcctg ctcttgtatt gatgcaactg gcatggggct ggcggacgat
     2041 gtttgtcatc atcggcgtag cagggattct ggttgggatc tgttggtacg cgtggtatcg
     2101 caaccgggcg cagtttgtcc tgaccgagga agaacggaca tatctctccg cgccggttaa
     2161 accgcgtcca cagttacaat ttagcgagtg gctggcgctg tttaagcatc ggacaacctg
     2221 ggggatgatt ttaggtttct ctggcgtcaa ttataccggt tggctctaca tcgcgtggtt
     2281 acccggttat ttgcaggccg aacaaggttt cagtctggcg aaaaccggtt gggtggcggc
     2341 aattcctttc ctcgcggcgg cggtcgggat gtgggttaac ggtattgttg tcgatcggct
     2401 ggcgaaaaaa ggctatgacc tggcgaagac gcgtaaaacg gcgattgtct gcggtttgat
     2461 gatgtcggca ttaggcacgt tgctggtcgt gcaatcttcc tcgccagccc aggcggtagc
     2521 gtttatctca atggcgctgt tctgcgtgca tttcgcggga acatctgcat gggggctggt
     2581 gcaggtgatg gtgtcagaaa caaaagtggc ttccatcgcc gggattcaaa actttggcag
     2641 ttttgttttt gcttcctttg ctccgatcgt aaccggttgg gtcgtggata ccacacactc
     2701 gtttaatctg gcgctggtta tcgcggcctg tgtgacgttc accggggcgc tgtgttactt
     2761 ctttattgtc aaagatcgga ttgagtaacc gttactatca ggctctcttg ctgtgggcct
     2821 gtagaataaa aaaaagcgga tcctggagat ccgcaaaagt tcacgttggc tttagttatt
     2881 cgagttgaga aactctcgaa acgggcagtg acttcaaggg ttaaaagagg tgccgctccg
     2941 tttctgtgag caattatcag tcagaatgct tgatagggat aatcgttcgt tgctatgcta
     3001 cctatcgccg cgaactatcg tggcaatgga ggatggataa tgaatattcg tgatcttgag
     3061 tacctggtgg cattggccga acaccgccat tttcggcgtg cggcagattc ctgccacgtt
     3121 agccagccga cgcttagcgg gcaaattcgt aagctggaag atgagttggg cgtgatgttg
     3181 ctggagcgga ccagccgtaa agtgttgttc acccaggcgg gaatgctgct ggtggatcag
     3241 gcgcgtaccg tgctgcgtga ggtgaaagtc cttaaagaga tggcaagcca gcagggtgag
     3301 acgatgtccg gaccgctgca tattggtttg attcccacgg ttggaccgta cctgctaccg
     3361 catattatcc cgatgctgca ccagaccttt ccaaagctgg aaatgtatct gcatgaagca
     3421 cagacccacc agttactggc gcaactggac agcggcaaac tcgattgcgt gatcctcgcg
     3481 ctggtgaaag agagcgaagc attcattgaa gtgccgttgt ttgatgagcc aatgttgctg
     3541 gctatctatg aagatcaccc gtgggcgaac cgcgaatgtg taccgatggc cgatctggca
     3601 ggggaaaaac tgctgatgct ggaagatggt cactgtttgc gcgatcaggc aatgggtttc
     3661 tgctttgaag ccggggcgga tgaagataca cacttccgcg cgaccagcct ggaaactctg
     3721 cgcaacatgg tggcggcagg tagcgggatc actttactgc ctgcgctggc tgtgccgccg
     3781 gaacgcaaac gcgatggggt tgtttatctg ccgtgcatta agccggaacc acgccgcact
     3841 attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc gctatgagca gctggcagag
     3901 gccatccgcg caagaatgga tggccatttc gataaagtgt taaaacaggc ggtttaagcc
     3961 atttagcgca gctacccgat aggcttccgc catcgtcggg tagttaaagg tggtgttgac
     4021 gaagtactca atagtgttgc cgccaccttt ctgttccata atcgcctgac cgatatgaat
     4081 aatttcggca gcgcgctcgc caaagcagtg aatacccaga atctcttttg tttcccgatg
     4141 gaacaaaatt ttcagcgtgc ccacgttcat gccgacgatt tgtgcacgtg ccagatgttt
     4201 aaactgggcg cggcccactt catatggcac tttcatcgcg gtcagctgct gttcggtttt
     4261 gcctacagag ctgatttccg ggatggtgta aatgccggta gggatatctt caatcagatg
     4321 tgcggtggct tcacctttca ccagcgcctg cgcggcaatg cgcccctggt cataggctgc
     4381 cgacgccagg ctcggataac caatcacgtc gcccaccgcg taaacgtgcg gctgtgcggt
     4441 ctgatacatg ctgttgacct tcagctgtcc gcgactgtca gtttccagcc caatgttctg
     4501 taacgccagc gaatcggtat taccggtgcg accgttggca tagagcagac agtcagcttt
     4561 cagtttttta cccgacttca gatgcatgat cacaccgtcg tcacagcctt cgatcttctc
     4621 gtactcttcg ttgtgacgaa tcactacgcc actgttccag aagtgatagg agagagaatc
     4681 tgacatctct tgatcgagaa acgccagcag acgatcgcgg gtgttgatca gatccacttt
     4741 tacatccata ccgcggaaga tcgacgcata ttcacagccg atcactccag caccgtaaat
     4801 aagtacatgg cgcggttcgt ggtgcatgct aagaattgag tcgctgtcat aaatgcgtgg
     4861 atgggtgaaa tcaacatctg ttggatgata tggacgagaa ccgcaggcaa taacaaattt
     4921 ttcagcagtt agtgtttcga cgctgccgtc cgggcaatcc agcgccaacg tatgctcatc
     4981 aacaaagcga gcgttcccct gcaatatttc acagtgatta cgttcgtaaa atccctgacg
     5041 catgcgcgtt tgttgattaa tcacgttatc ggcatggtta aggatatcgg caaaagaaga
     5101 gcggagcagt cgggaatggt cgctgtatag tgggttttga ttgaattcta taatgcggct
     5161 gacggcgtga cggagagctt tcgacgggat ggtgccccag tgggtgcaac cgccgccaac
     5221 attttgataa cgctcgataa ctgcgacgcg cgcaccttgc ttaaccaggc ccatcgcagc
     5281 gccttcgccg ccggggccgg aacctattac tatggcatcg taatcgtagg aatgtggcat
     5341 ggtagggctt acctgttctt atacataaaa gcaacagaat ggtaacattt tatcgcgggt
     5401 aagccaattg atccccgtca tttatctggc tatagcgtga gcggcctttg ctttgtcttc
     5461 ctccttctta ttttatttat tcactattaa tcgctttttc ttatggctga tagtt
//
LOCUS       DADTFG010000213         5488 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.214, whole genome shotgun sequence.
ACCESSION   DADTFG010000213 DADTFG010000000
VERSION     DADTFG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.214"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            246..2042
                     /locus_tag="J1138_004371"
     CDS             246..2042
                     /locus_tag="J1138_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002954510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HBA8456067.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKSVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            2060..2728
                     /locus_tag="J1138_004372"
     CDS             2060..2728
                     /locus_tag="J1138_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HBA8456068.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2743..3414
                     /gene="dsbI"
                     /locus_tag="J1138_004373"
     CDS             2743..3414
                     /gene="dsbI"
                     /locus_tag="J1138_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HBA8456069.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3460..4275)
                     /gene="ygiD"
                     /locus_tag="J1138_004374"
     CDS             complement(3460..4275)
                     /gene="ygiD"
                     /locus_tag="J1138_004374"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBA8456070.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTHYPAPGSPALAQHLV
                     ELLAPVPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4391..5164
                     /gene="zupT"
                     /locus_tag="J1138_004375"
     CDS             4391..5164
                     /gene="zupT"
                     /locus_tag="J1138_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBA8456071.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5226..5396)
                     /locus_tag="J1138_004376"
     CDS             complement(5226..5396)
                     /locus_tag="J1138_004376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456072.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
BASE COUNT         1454 a         1328 c         1303 g         1403 t
ORIGIN      
        1 aaaaacaatg ttgcagggat cacactacac agtattgaac attgtgattg atatattgcc
       61 cccctactat ccattaaaca taaatatttg tttcaccgaa taacaacata tttaaaagaa
      121 acacaaaagc attctcactc tttattgaat taatatttat attcacgtaa cgaatttatt
      181 agcaccctct caacagtcga tattcatcat tattatatat ttgcgttata taaggagata
      241 tatccatgtt tgataaatat agaaaaacac tcgtagccgg aactgtggcg ataaccctgg
      301 gtttgtcagc atcgggggtg atggctgcgg gttttaaacc agcgccgcct gccgggcaac
      361 tgggtgcggt cattgtcgat ccctacggca atgcaccact gaccgctttg gttgacttag
      421 atagccatgt tatttctgac gtcaaagtta ccgtccatgg gaagggcgaa aaaggcgtag
      481 aaatcagcta tcccgtgggt caggaatcac taaaaactta cgatggtgta ccgatttttg
      541 gtctttatca gaaatttgct aacaaagtga ccgttgagtg gaaagaaaac ggcaaggtca
      601 tgaaagatga ttatgtggtg cacacttcgg ccatcgtcaa taattacatg gataaccgct
      661 ctatctccga tttacaacag accaaagtta ttaaagtcgc accgggtttt gaagatcgcc
      721 tctatctggt taatacccac acctttaccg cccaaggttc cgatctccac tggcatggtg
      781 agaaagataa aaatgccggt atccttgatg cgggtccggc aaccggcgca ctcccttttg
      841 atatcgcgcc ctttactttc atcgtcgata ccgaaggtga ataccgctgg tggctggatc
      901 aagacacttt ctacgatggt cgtgaccgcg atatcaacaa gcgtggttat ctgatgggta
      961 tccgcgaaac gccacgcggc acctttaccg ctgtacaagg tcagcactgg tacgagttcg
     1021 acatgatggg gcaggtgctc gaagatcaca aactgccgcg cggatttgct gacgccactc
     1081 atgagtccat tgagacacca aatggcacgg tactgttgcg cgtaggtaag agtaactatc
     1141 gtcgcgatga cggcgtacac gtcaccacca ttcgtgacca tatcctcgaa gttgataaat
     1201 ctggtcgcgt tgtcgatgta tgggatctga ccaagatcct cgatccgaaa cgcgatgcac
     1261 tgctcggcgc gctggatgca ggtgcagttt gcgttaacgt tgaccttgcc catgcaggac
     1321 aacaggcaaa actggaacca gatacaccgt tcggcgacgc gctgggtgta gggccaggcc
     1381 gcaactgggc gcacgtgaac tctatcgcct atgacgcaaa agatgactct atcatcctct
     1441 cctctcgcca ccagggtgtt gtgaagattg gtcgtgataa gcaagtgaaa tggatccttg
     1501 cgccctctaa aggttgggaa aaaccgctgg ccagcaagct gctgaaatcg gtggacgcta
     1561 acggtaaacc tattacctgt aacgaaaacg gtctgtgtga aaactcggac ttcgatttta
     1621 cctacaccca gcataccgcc tggatttcca gcaaaggaac gctcaccatt tttgataatg
     1681 gcgatgggcg tcatctggaa caacctgcct taccaaccat gaaatactcc cgctttgtgg
     1741 aatataagat tgatgagaag aaaggcaccg ttcagcaagt gtgggaatac ggtaaagaac
     1801 gtggctacga tttctatagc ccgattacct ccatcattga atatcaagcc gaccgtaaca
     1861 ccatgtttgg cttcggtggt tctattcatt tgttcgatgt cgggcagcca accgtcggta
     1921 agttgaacga aatcgattac aaaaccaaag aagtgaaagt ggaaatcgac gtgctgtcag
     1981 ataaacccaa tcagactcac taccgtgcgc tgttagtccg tccacaacag atgttcaaat
     2041 aattaagaag ggaataaaaa tgtcgaaatt agggatctca tcactattta aaaccatact
     2101 gcttaccgca gctctggcgg tttcatttac cgcttctgca tttaccgaag gcacggatta
     2161 catggttctg gaaaaaccaa ttccgaatgc cgacaaaacg ctgattaagg tatttagcta
     2221 cgcctgcccg ttctgttaca agtacgacaa agcggttacc ggtccggtat cggaaaaagt
     2281 gaaagatatc gttgctttca cgccgttcca tctggaaaca aaaggtgaat acggcaaaca
     2341 agcaagcgaa gtctttgcgg tcttgatcaa taaagataaa gcagcaggca tttccttatt
     2401 tgatgctaac tctcagttta agaaagccaa gtttgcctac tacgcggctt atcacgacaa
     2461 aaaagaacgc tggtctgacg gtaaagatcc ggctgctttt atcaaaaccg gtctggatgc
     2521 cgcaggtatg agtcaggcgg attttgaagc ggcactgaaa gaaccagcag ttcaagaaac
     2581 actggaaaaa tggaaagcct cttatgatgt cgccaaaatc cagggggttc cagcctatgt
     2641 cgttaatggt aagtacttga tctacaccaa gagcatcaaa tccatcgacg cgatggcaga
     2701 ccttatccgt gagttagcaa gcaaataaca gggaaacagg ctatgggtat taagggaatg
     2761 tggaaagatc ttcgcacatc accagttgac acactggtca gatggcagga gcaacgcttg
     2821 ttgtggttgt taatggccgt tgcgatgggc gcgttaatta tcctggcgca ctctttcttc
     2881 cagatctatc tctacatggc accctgtgag caatgtgtat acattcgcta cgccatgttt
     2941 gtgatggtta ttggaggatt ggttgcagcg atcaacccaa agaacatcat cctaaagcta
     3001 attggctgcg tgatggcatt ttacggcagc attttgggac tgaagttttc gctcaaactg
     3061 aacgatatcc accatgcagt acataatccg gatcccgatt cactgtttgg cgtacagggt
     3121 tgctctaccg atccaacttt tccctttaac ctgccgctgg cacagtgggc accaaattgg
     3181 ttcaagccta ccggggattg tggctatgac gcaccaatcg ttcctgatgg cgtcacactg
     3241 agcagcacgc aacagtggtt tgtggaaatg tatcagcaat ctgaaggctg gtatctgcta
     3301 ccaccatggc attttatgaa tatggctcag gcttgtatgc tggcttttgg tatgtgtctg
     3361 gtgctgctgg tcattatgag tggcgcatgg gcgcttaaaa tcatcagagg ctaactcagc
     3421 aacgcccgaa gccagtcatt ctggcttcgg gttcttttat taacctatct gcaccgacag
     3481 catacttaaa ctgcccattt ctataccatc gaccggaatg gtaattggct cctgcccatc
     3541 ccacgcacct aagacataca acaacggcag atagtgctct ggcgttgggt tcgataacgc
     3601 gccaccttca tggtcgagat aattcaccag aggatgttgt tccaccggcc cttgccacgt
     3661 cagattcgct ttcacatact cattaaacga cgtcgcccat ggatacggtg aactatcacc
     3721 gtgccacttc actgtacgca ggttatgtac cacgttgcca ctggcgacca acattatccc
     3781 ttcatcacgc agcgccgcga gtttgcgccc catttcgaaa tgccaggcgg caggtttgct
     3841 actgtcgata ctcaactgca ccatcgggat atcggcgtca ggatacatct taatcagtac
     3901 gccccacgag ccgtggtcga agccccaggc ttctttatcc agcgtcacag ggaccggcgc
     3961 taacagctca accagatgct gtgccagcgc aggtgaaccc ggagcaggat aatgcgtatc
     4021 gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg ggcggcatct ccatcgcggt
     4081 cactcctgtt ccacgggtaa accaatgagc cgaaaccacc actattgctt gcgggcgtgg
     4141 caatgtcatc cccaacgtct gccagctgcg ggtatacaaa ttatcttcca gcacgttcat
     4201 cggactaccg tgacctaaaa acaatgctgg catacgtgtt gaagacatga tgatatcctt
     4261 aactaaaggt gtcattttga tatcctcaca atacgcttgt tcggcggagt aagaacccgg
     4321 ataacaatga tgatgatcat cagttatttt gacgatctgc ctgaaggtaa agatttataa
     4381 ggagttgtcg atgtcagtac ctctcattct gaccatactg gcgggggcag ccacgtttat
     4441 tggcgcgttt ctcggcgttc tcgggcaaaa accctcgaac cgcttactgg cgttttcgct
     4501 aggatttgcg gcggggatca tgttgctcat ctcattaatg gaaatgcttc ctgccgcatt
     4561 agccgccgaa ggaatgtcgc ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg
     4621 ctattttggc ctggaccgca tgttgccaca tgcgcatccg caggatttaa tgcaaaaatc
     4681 ggtgcagccg ttgccaaaat cgatcaagcg cacagccatt ctgctcactc tcggcatcag
     4741 tctgcataac ttcccggaag ggattgccac ctttgtcacg gcgagcagca acctggagct
     4801 gggatttggc atcgccctgg ccgtagcgtt gcacaatatt cctgaaggtc tggcggttgc
     4861 aggcccggtt tacgctgcaa caggatcgaa acgtaccgcg attctgtggg cgggcatttc
     4921 cgggctggca gaaattcttg gtggtgtgct ggcatggtta atcctcggta gcatgatttc
     4981 tccggtggtc atggcggcaa tcatggcggc ggttgcagga attatggtgg cgctctccgt
     5041 tgacgaatta atgccgctcg ccaaagagat cgaccccaat aataacccca gctatggcgt
     5101 actatgtgga atgtcagtga tgggattcag tttagtgctg ctacaaacgg cgggaattgg
     5161 ttaaagacga taatgacaga tgttgcccat tgctaaaggc aacatctgta tcgttattaa
     5221 aggtattatt gatgcttttc aaacacttct ttatcggagc gtttatctaa tttacttcgg
     5281 gcagctaaca gcgcatctct gtcatggcga aaagctttac agtaacgttt aagatgaaga
     5341 aaatagccac cagccagtag aacaattaat acaatccagt accaggcaat aaacatcctt
     5401 ttcaccttca aaatttaaca ttactaaaca tttagcgtta aaatttcaca tatccatcgt
     5461 tgaatacatt catatactcg cataattc
//
LOCUS       DADTFG010000214         5375 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.215, whole genome shotgun sequence.
ACCESSION   DADTFG010000214 DADTFG010000000
VERSION     DADTFG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.215"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(78..1361)
                     /gene="gltA"
                     /locus_tag="J1138_004377"
     CDS             complement(78..1361)
                     /gene="gltA"
                     /locus_tag="J1138_004377"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBA8456073.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1567..1674)
                     /locus_tag="J1138_004378"
     CDS             complement(1567..1674)
                     /locus_tag="J1138_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456074.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2055..2459
                     /gene="sdhC"
                     /locus_tag="J1138_004379"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2055..2459
                     /gene="sdhC"
                     /locus_tag="J1138_004379"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA8456075.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2453..2800
                     /gene="sdhD"
                     /locus_tag="J1138_004380"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2453..2800
                     /gene="sdhD"
                     /locus_tag="J1138_004380"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBA8456076.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2800..4566
                     /gene="sdhA"
                     /locus_tag="J1138_004381"
     CDS             2800..4566
                     /gene="sdhA"
                     /locus_tag="J1138_004381"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBA8456077.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4582..5298
                     /locus_tag="J1138_004382"
     CDS             4582..5298
                     /locus_tag="J1138_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HBA8456078.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
BASE COUNT         1264 a         1325 c         1442 g         1344 t
ORIGIN      
        1 ttttatgttc agccatataa aaagaacccg ctctattaaa ggcgggtccg gaaagtgaac
       61 ggcttagcaa tcaaccatta acgcttgata tcgcttttaa agtcgcgttt ttcatatcct
      121 gtatacagct gacgcggacg ggcaatcttc ataccgtcac tgtgcatttc gctccagtgg
      181 gcgatccagc caaccgtacg tgccatagcg aagatcacgg tgaacataga agacggaata
      241 cccatcgctt tcaggatgat accagagtag aaatcgacgt tcgggtacag ttttttctcg
      301 ataaagtacg ggtcgttcag cgcgatgttt tccagctcca tagccacttc cagcaggtca
      361 tctttggtgc ccagctcttt cagcacttca tggcaggttt cacgcattac ggtggcgcgc
      421 gggtcgtagt ttttgtacac gcgatgaccg aagcccatca ggcggaaaga atcatttttg
      481 tctttcgcac gacgaacaaa ttccggaatg tgtttaacgg agctgatttc ttccagcatt
      541 ttcagcgccg cttcgttagc accaccgtgc gcaggtcccc acagtgaagc aatacctgct
      601 gcgatacagg caaacgggtt cgcacccgaa gagccagcgg tacgcacggt ggaggtggag
      661 gcgttctgtt catggtcagc gtgcaggatc agaatacggt ccatagcacg ttccagaatc
      721 ggattaactt catacggttc gcacggcgtg gagaacatca tattcaggaa gttaccggcg
      781 taggagagat cgttgcgcgg gtaaacaaat ggctgaccaa tggaatactt gtaacacatc
      841 gcggccatgg tcggcatttt tgacagcagg cggaacgcgg caatttcacg atgacgagga
      901 ttgttaacat ccagcgagtc gtgatagaac gctgccagcg cgccggtaat accacacatg
      961 actgccattg gatgcgagtc gcgacggaaa gcatggaaca gacgggtaat ctgctcgtgg
     1021 atcatagtat ggcgggtcac cgtagtttta aattcgtcat actgttcctg agtcggtttt
     1081 tcaccattca gcaggatgta acaaacttcc aggtagttag aatcggtcgc cagctgatcg
     1141 atcgggaaac cgcggtgcag caaaatacct tcatcaccat caataaaagt aattttagat
     1201 tcgcaggatg cggttgaagt gaagcctggg tcaaaggtga acacaccttt tgaaccgaga
     1261 gtacggatat caataacatc ttgacccagc gtgcctttca gcacatccag ttcaacagct
     1321 gtgtccccgt tgagggtgag ttttgctttt gtatcagcca tttaaggtct ccttagcgcc
     1381 ttattgcgta agactgccgg aacttaaatt tgccttcgca catcaacctg gctttacccg
     1441 tttgttattt ggctcgccgc tctgtgaaag aggggaaaac ctgggtacag agctctgggc
     1501 gcttgcaggt aaaggatcta ttgatgacga ataaatggcg aatcaagtac ttagcaatcc
     1561 gaattattaa acttgtctac cactaataac tgtcccgaat gaattggtca atacttccac
     1621 actgttacat aagttaatct taggtgaaat accgacttca taacttttac gcattatatg
     1681 ctttttctgg taatgtttgt aacaactttg ttgaatgatt gtcaaattag atgattaaaa
     1741 attaaataga tgttgttatc gtgacgcgga tcactgttcg ggataaaacc cgacaaacta
     1801 tatgtaggtt aattgtaatg attttgtgaa cggcctatac tgccgccagg tctccggaac
     1861 accctgcaat cccgagccac ccagcgttgt aacgtgtcgt tttcgcatct ggaagcagtg
     1921 ttttgcatga cgcgcagtta tagaaaggac gctgtctgac ccgcaagcag accggaggaa
     1981 ggaaatcccg acgtctcctg gtaacagaaa gttaacctct gtgcccgtag tccccaggga
     2041 ataataagaa cagcatgtgg gcgttattca tgataagaaa tgtgaaaaaa caaagacctg
     2101 ttaatctgga cctacagacc atccggttcc ccgtcacggc gatagcgtcc attctccatc
     2161 gcgtttccgg tgtgatcacc tttgttgcag tgggcatcct gctgtggctt ctgggtacca
     2221 gcctctcttc ccctgaaggt ttcgagcaag cttccgcgat tatgggcagc ttcttcgtca
     2281 aatttatcat gtggggcatc cttaccgctc tggcatatca cgtcgtcgta ggtattcgcc
     2341 acatgatgat ggattttggc tatctggaag aaacattcga agcgggtaaa cgctccgcca
     2401 aaatctcctt tgttattact gtcgtgcttt cacttctcgc aggagtcctc gtatggtaag
     2461 caacgcctcc gcattaggac gcaatggcgt acatgatttc atcctcgttc gtgctaccgc
     2521 tatcgtcctg acgctctaca tcatttatat ggtcggtttt ttcgctacca gtggcgagct
     2581 gacatatgaa gtctggattg gtttcttcgc ctctgcgttc accaaagtgt tcaccctgct
     2641 ggcgctgttt tctatcttga tccatgcctg gatcggcatg tggcaggtgt tgaccgacta
     2701 cgttaaaccg ctggccttgc gcctgatgct gcaactggtg attgtcgttg cactggtggt
     2761 ttacgtgatt tatggattcg ttgtggtgtg gggtgtgtga tgaaattgcc agtcagagaa
     2821 tttgatgcag ttgtgattgg tgctggcggc gcaggtatgc gcgcggcgct gcaaatttcc
     2881 cagagtggcc agacctgtgc gctgctctct aaagtcttcc cgacccgttc ccataccgtt
     2941 tctgcgcaag gtggtattac cgttgcgctg ggtaataccc atgaagataa ctgggaatgg
     3001 catatgtacg acaccgtaaa agggtcggac tatatcggtg accaggacgc gattgaatat
     3061 atgtgtaaaa ccgggccgga agcgattctg gaactggaac atatgggcct gccgttctcg
     3121 cgtcttgatg atggtcgtat ctatcaacgt ccgtttggcg gtcagtcgaa aaacttcggc
     3181 ggcgagcagg cggcacgtac tgcggcggct gccgaccgta ccggtcacgc actgttgcac
     3241 accctttatc agcagaacct gaaaaaccac accaccattt tctccgagtg gtatgcgctg
     3301 gatctggtga aaaaccagga tggcgcagtg gtcggttgta ccgcactgtg catcgaaact
     3361 ggtgaagtgg tttactttaa agctcgcgcg acagtgctgg cgactggcgg ggcagggcgt
     3421 atttatcagt ccaccactaa cgcccacatt aacactggcg acggtgtcgg catggctatc
     3481 cgtgcaggcg taccggtaca ggatatggaa atgtggcagt tccacccgac cggtattgcc
     3541 ggtgcgggcg tactggtcac cgaaggttgc cgtggtgaag gcggttatct gctgaacaaa
     3601 catggcgaac gctttatgga gcgttatgcg ccgaacgcca aagacctggc gggccgtgac
     3661 gtggtggcgc gttccatcat gatcgaaatc cgtgaaggcc gcggctgtga tggtccgtgg
     3721 gggccacacg caaaactgaa acttgaccat ctggggaaag aagttctcga atcccgtctg
     3781 ccgggtattc tggaactctc ccgtaccttc gctcacgtcg atccggtgaa agagccgatt
     3841 ccggttatcc caacctgtca ctacatgatg ggcggtattc cgaccaaagt gaccggtcag
     3901 gcgctgacgg tgaatgagaa aggcgaagat gtggttgttc cggggctgtt tgccgttggt
     3961 gaaatcgctt gtgtatcggt acacggcgct aaccgtctgg gcggcaactc gctgctggac
     4021 ctggtcgtat ttggtcgtgc ggcaggtctg catctgcaag agtctatcgc cgagcagggc
     4081 gcactgcgcg atgccagcga gtctgatgta gaagcgtctc tggatcgcct gaaccgctgg
     4141 aacaataacc gtaacggtga agatccggtg gcgatccgta aagcactgca agaatgtatg
     4201 cagcataact tctcggtctt ccgtgaaggt gatgcgatgg cgaaagggct tgagcagttg
     4261 aaagttatcc gcgagcgtct gaaaaatgcc cgtctggatg acacttccag cgagttcaac
     4321 acccagcgcg ttgagtgcct ggaactggat aacctgatgg aaacggcgta tgcaacggct
     4381 gtttctgcca acttccgtac cgaaagccgt ggcgcgcata gccgcttcga cttcccggat
     4441 cgcgatgatg aaaactggct gtgccactcc ctgtatctgc cagagtcgga atccatgacg
     4501 cgccgaagcg tcaacatgga accgaaactg cgcccggcat tcccgccgaa gattcgtact
     4561 tactaatgcg gagacaggaa aatgagactc gagttttcaa tttatcgcta taacccggat
     4621 gttgatgatg ctccgcgtat gcaggattac accctggaag cggaagaagg tcgcgacatg
     4681 atgctgctgg atgcgcttat tcagctgaaa gagaaagatc ccagcctgtc gttccgccgc
     4741 tcctgccgtg aaggtgtgtg cggttccgac ggtctgaaca tgaacggtaa gaatggtctg
     4801 gcctgtatta ctccgatttc ggcactcaac cagccgggca agaagattgt gattcgcccg
     4861 ctgccaggtt taccggtgat ccgcgatttg gtggtagaca tgggacaatt ctatgcgcaa
     4921 tatgagaaaa ttaagcctta cctgttgaat aatggacaaa atccgccagc tcgcgagcat
     4981 ttacagatgc cagagcagcg cgaaaaactc gacgggttgt atgaatgtat tctctgcgca
     5041 tgttgttcaa cctcttgtcc gtctttctgg tggaatcccg ataagtttat cggcccggca
     5101 ggcttgttag cggcatatcg tttcctgatc gatagccgtg ataccgagac tgacagccgc
     5161 ctcgacggtt tgagcgatgc attcagtgta ttccgctgtc acagcatcat gaactgcgtc
     5221 agtgtatgtc cgaaggggct gaacccgacg cgcgccatcg gccatatcaa gtcgatgttg
     5281 ttgcaacgta atgcgtaaac ccaggcctga taagacgcgc aagcgtcgca tcaggcaacc
     5341 agtgccggat gcggcgtgaa cgccttatcc ggcct
//
LOCUS       DADTFG010000215         5363 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.216, whole genome shotgun sequence.
ACCESSION   DADTFG010000215 DADTFG010000000
VERSION     DADTFG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.216"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            112..1470
                     /gene="mltD"
                     /locus_tag="J1138_004383"
     CDS             112..1470
                     /gene="mltD"
                     /locus_tag="J1138_004383"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBA8456079.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIVAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1519..2289)
                     /locus_tag="J1138_004384"
     CDS             complement(1519..2289)
                     /locus_tag="J1138_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA8456080.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGKLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHTRNYASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(2367..3167)
                     /locus_tag="J1138_004385"
     CDS             complement(2367..3167)
                     /locus_tag="J1138_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBA8456081.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3408..4322
                     /locus_tag="J1138_004386"
     CDS             3408..4322
                     /locus_tag="J1138_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8456082.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISCYGKPETIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4319..5122)
                     /gene="dkgB"
                     /locus_tag="J1138_004387"
     CDS             complement(4319..5122)
                     /gene="dkgB"
                     /locus_tag="J1138_004387"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBA8456083.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5285..5361)
                     /locus_tag="J1138_004388"
     tRNA            complement(5285..5361)
                     /locus_tag="J1138_004388"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5325..5327),aa:Asp,seq:gtc)
BASE COUNT         1401 a         1292 c         1342 g         1328 t
ORIGIN      
        1 acgttttgca tggttaaggt caaagaaaga taggttctga taaaactttc ttgtcatcgg
       61 ctccgttcgc cgttatgatc ggtcgtcttt taagcaacta ttgacacaca catgaaggca
      121 aaagcgatat tactcgcctc tgtcctgctc gtgggttgcc agagcaccgg caacgttcaa
      181 cagcacgcac agagcctttc tgcagctggt caaggggaag cagcaaagtt tacaagtcag
      241 gcacgatgga tggacgatgg gacgtctatc gcgccagatg gtgacttgtg ggctttcatt
      301 ggcgacgagc taaagatggg aattccggaa aacgaccgga ttcgcgaaca gaaacagaaa
      361 tatttacgca ataagagcta tctccacgat gtaactttac gggcagagcc gtatatgtac
      421 tggatagccg ggcaagttaa aaaacgtaac atgcctatgg aactggtact actacccata
      481 gtggagagcg cttttgatcc tcacgcaacg tctggcgcca atgccgcggg catctggcag
      541 atcattccga gcacggggcg caattatggt ttgaaacaga cccgcaatta tgacgcgcgt
      601 cgcgatgttg ttgcttcaac aactgccgcg ctgaacatga tgcagcgtct gaacaagatg
      661 tttgacggcg actggcttct gaccgtagcg gcttataaca gcggcgaagg tcgggtcatg
      721 aaggcaatta aaacgaacaa agcgcgtggg aaatccacgg acttctggtc gttaccgttg
      781 ccgcaggaaa cgaagcagta cgtgcctaaa atgctggcat tgagtgatat tctcaaaaac
      841 agcaagcgtt atggcgtacg tctgccaacg accgatgaaa gccgtgctct ggcacgtgtg
      901 cacctgagca gcccggttga aatggcgaag gttgcagata tggcggggat ttccgtcagc
      961 aagctgaaga cattcaacgc aggcgtgaaa ggctccacgc tgggcgcaag tggtccgcag
     1021 tacgtgatgg tgccaaagaa gcatgcagat caactgcgtg aatctctggc ttcaggcgaa
     1081 attgttgctg tacagtcgac gctggttgcc gacaatacgc cgcttaacag ccgtgtttac
     1141 accgtacgct ctggcgacac gctttcaagt atcgcttcac gtctcggcgt aagcaccaaa
     1201 gatttgcagc aatggaacaa actgcgcgga tctaagctga agccaggcca aagtttgacg
     1261 attggtgcag gcagtagcgc acagcgactg gcaaacaaca gcgatagcat tacgtatcgt
     1321 gtgcgcaaag gcgattcgct ttcaagcatt gctaaacgcc acggcgtgaa catcaaagat
     1381 gtaatgcgct ggaacagcga cactgcgaat ctgcaaccag gcgataagct gacgttgttt
     1441 gtgaaaaaca acaacatgcc agattcctga caaaccagat aataaaaaag gcaccgattc
     1501 ccccggtgcc ttttttattt atgccgcttt atgtgcttct agcatgatga tatcactggt
     1561 gaaagagcca tcattctgca aggcaaaata cgttttcacc tctgtcgatg cgctctgttg
     1621 gtaaatgcga atagcgtcta ctaacgcttc tggcgtacgc attctcgcta cccatgaaga
     1681 aaattccagc ggtaacttat ctgtaattaa attatcaact atcagattgg cttcactgat
     1741 taacgtcaac cactcaccgc tggcgtagtt ccgtgtgtga gatgtatcgc gtaatgcttc
     1801 taccgtctgt aaccagatgt cgcgcactgg gtggcccgga gacattacgt ccatcacaat
     1861 cagcttaccg ccaggtttca atatccggtt cacttcacgc agtgctgcac caacatcatg
     1921 ccagtgatgg gcagaataac ggctgataac aatatcaaat gcgttatcgg caaatggcag
     1981 gctttcgaca taaccctggc gggtggtgat atttttcagt tgccgggctt cggcagcttg
     2041 tgccacgaca tccagcatat gggcagataa gtcatacgcc accaccgtgc tcacgttttg
     2101 cgcagcgaca aagctggcat gccctgctcc gcagcccata tcaagtacac ttgcatcagg
     2161 ataatcagcc agacgcaccg ccaggcgctg taaatctcgt ccggatgcat gtacggtact
     2221 ggtcagatat tcgctggctt gagagctaaa ctgcttttct acgtggtcat ggtgggattg
     2281 tgttgtcatt gcgctgtcct tttgttgttt gagtataaaa gggcagcgcc aaacgttggc
     2341 ctgccctgtg gcagacctga cataccttat ttatcaggct tgccgggact gaattcaacg
     2401 agtagcggat tgtgatcgga cgcgcgcgta accagtacag aagcttcgct gacgttcaga
     2461 ccacggtaga aaacaaaatc gagcgggcga ccaaacgccc gacggcgctg atcatcggta
     2521 aaacgcacct ggcgtagcga catttcccgc gcaaagcgat ataacgcgtt catccttcta
     2581 cggctccagg cattgaaatc tcccgccata atgaccgggc cactgtggtg agctatctga
     2641 tcgccaatag gaagtaactg cttactatag acatccacgc ccagactgaa gttgacggcg
     2701 tgtatattaa ccaccatcaa caggcgggtg tcaggtaagg gatagaccgt caccagtgcc
     2761 gatttcgcca gacgcaaaat gggttctcgt tcacgtaacg ggcagcaata cactggatgt
     2821 gccgccgaaa gggtcattac gccagaagga tgttgtggca gcacgaaagc gggtacctga
     2881 tcggcggcaa gatagttagc agtcgcaaac tgtactaact ctggcgttgt ctgcgcttcc
     2941 tgcaataaca ccagatgtgc atctttgccg tagttcttta ataccgacaa ccattcagcg
     3001 cgttgctgtt tgtatatgtt ccacaccagg atccgaatac gctcttcggt acttaacggt
     3061 tctccaggtg gtaatgcctg gccgatgctc gcaaaagacc ccggcggtaa gatcctttcc
     3121 gctggttgtc cggcaacata gcgcatggca taggtgtttt ttcgcacttt tgactttgaa
     3181 acctctatca cgtcaaccct tccgcactcg ggagggttct actgttatag ggattttagc
     3241 tcactctttc aacgagcaat tgtatattgt tatgtaagca agtgcttaca ccggtcagga
     3301 ttttgctcat tattgagaac attaaatcgg atggcatatc tcgcatcctg atggtttaat
     3361 gacaaataaa ttcaaaatat ctcttctatt ttgcaacagg agcaaaaatg aaagccacgt
     3421 cggaagaact cgccattttt gtttcagtcg tcgaaagcgg cagctttagc cgggcagcgg
     3481 aacaattagg gcaagcaaac tcagcggtaa gccgggcggt gaaaaagctg gagatgaaac
     3541 ttggcgttag cctgcttaat cggaccacgc ggcaacttag cctgacggaa gaaggcgagc
     3601 gttattttcg tcgcgtacag tcaattttgc aggagatggc agcggcagaa tcagaaatta
     3661 tggagacgcg taatacaccg cgtggactgt tacggatcga tgccgcaact ccagtggtac
     3721 tgcactttct gatgccgttg attaagcctt ttcgtgaacg ctatccggaa gtcactttat
     3781 cgctagtctc ctccgaaacg attattaatt tgatcgaaag aaaagtggat gtcgcgatac
     3841 gcgccggtac gttaacggat tccagcttac gtgccaggcc gttatttaac agttatcgaa
     3901 aaattatcgc ctcccccgat tatatttcct gctacgggaa gccagaaacg atcgacgatt
     3961 taaagcaaca tatttgcctg agattcactg aacccgcttc cctcaatacc tggccgatag
     4021 cctgtagcga tggacaatta catgaggtga agtacggttt gtcatccaat agtggggaaa
     4081 cactgaaaca gctttgcctg agtggcaacg ggattgcgtg tttgtccgac tacatgatcg
     4141 acaaagaaat cgctcgcgga gaattggtgg agttaatggc agataaagtg ttgccagtgg
     4201 aaatgccatt cagtgcagtc tattacagcg accgtgcggt aagtacgcgc atccgggctt
     4261 ttatcgattt ccttagcgag catgtaaaaa cagctcccgg aggagctgtc agagaggctt
     4321 aatcccattc aggagccaga ccttccgggc taaccaggcg gtcgttgcaa tccagtgcgg
     4381 cgatcgcttt tttatcttcg gcatcaagct gtaaattttg tgccttaaga ttactctcca
     4441 ggttttcacg tttagtagaa gaaggaatta ctgagtaacc ttcccccata gcccacgcca
     4501 gaatcacttg agccggagtc gcattgtgtt tagctgcaat acgagcaata accttatctt
     4561 tcagtgcatt gccatacgcc agagtcatat aggaagtaat atggataccg tgctgtttag
     4621 cccaagcgac cactttacgg ttttgcagat aaggagagag ttcaatctgg ttagtagcga
     4681 tgttttcagc gccaacaaca gcaatcgctt tttccatcaa tgggatcgtg aagttggaaa
     4741 taccgatctc acgcgtcagc ccttgttttt tggcttccag cagtgcctgc ataaactctt
     4801 cagcagagac ttcatcgctt ggtgacggcc agtggattag cgtcagatca acataatcgg
     4861 tacgcaattt ttgcaggctc tctttcagac tcgggatcaa tttgtctttg ctgagatttt
     4921 caatccagat tttagtggtg atgtagagtt catgacgtgg cacgccactt tctgcaatcg
     4981 cctgacctac tgcggcttcg ttatcataga tttgtgcggt atcaattgcg cgataatcaa
     5041 gttcaagcgc cgttttcaca gatgaaataa caacgtcgtc tttcagacgg aaagtgccta
     5101 aaccaaatgc agggatagcc ataatatgcc tcttttaatg ctattttcgt taactgaggt
     5161 gattatgggc ttcagactgt tgtggaaaaa tggcgtaaaa cgcagaggat ttttgcgatt
     5221 ttggcaataa tagaaataca aacgaaaaaa aacctcgagc gttaactcga ggcttcttaa
     5281 taagtggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct
     5341 aaccgactga actaccgctc cac
//
LOCUS       DADTFG010000216         5326 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.217, whole genome shotgun sequence.
ACCESSION   DADTFG010000216 DADTFG010000000
VERSION     DADTFG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.217"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            1603..3741
                     /locus_tag="J1138_004389"
                     /pseudo
     CDS             1603..3741
                     /locus_tag="J1138_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            3741..4022
                     /locus_tag="J1138_004390"
     CDS             3741..4022
                     /locus_tag="J1138_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456084.1"
                     /translation="MKDLTLKFADRADFSAFMESIGYYDDESMQDDILIDVIGNVYKE
                     TGELTEDGEPVCVKEDGYFVNVRIINDPQISSLFDEYVVAVEHQLRGWM"
     gene            4032..5072
                     /locus_tag="J1138_004391"
     CDS             4032..5072
                     /locus_tag="J1138_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HBA8456085.1"
                     /translation="MATSTVIPDDIKTLKSDVSKLKNDQGSYATKLYVDSKDEIVGDW
                     SASWYQQVLPTSGAIFGRKLRSTHRTAGVEDAYCELYLKKWIDSPGNAMARLNLNDNG
                     TNICWDFTNLYGGTMIFPGDSGYLKMGNCLMSYSKRGSNALIKFDYTDTLQIKYANHG
                     STMTLNTQGTAYAGVTAQLWGNSSRPVVYEVGVDGGAYMFYAQKNTDNTYMLSVNGAC
                     HATAFNQHSDRDLKDNIQVIDNATDRIRKMNGYTYTLKENGMPYAGVIAQEALEAIPE
                     VVGSAMKYQDGASGSEGEEGERYYTVDYSGVTGLLVQVARESDDRITALEEENAELRQ
                     RLSAIEAALVSK"
     gene            5115..>5326
                     /locus_tag="J1138_004392"
     CDS             5115..>5326
                     /locus_tag="J1138_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456086.1"
                     /translation="MDITPFLHALCAVAAQLLIGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRA"
BASE COUNT         1455 a         1220 c         1507 g         1144 t
ORIGIN      
        1 gcactggtcg gcgtgcaggt ggactcggaa cagttcggca gccagcaggt gagccgtaat
       61 tatcattttc gcgggcgcat cctgcaggtg ccgtcgaact ataacccaca gacgcggcaa
      121 tacagcggta tctgggacgg aacgtttaag ccagcataca gcaacaacat ggcctggtgt
      181 ctgtgggata tgctgaccca cccgcgctac ggcatgggta aacgtcttgg tgcggcggat
      241 gtggacaaat gggcgctgta tgtcatcggc cagaattgcg accagtcggt gccggacggt
      301 tttggtggca cggagccgcg catttcctgt aatgcctggc tgaccacaca gcgtaaggcg
      361 tgggatgttc tcagtgattt ctgctcggcg atgcgctgta tgccggtatg gaacgggcag
      421 acgctgacgt tcgtgcagga ccgaccgtcg gataaggtgt ggacctataa ccgcagtaat
      481 gtggtgatgc cggatgatgg cgcgccgttc cgctacagct tcagcgccct gaaggaccgc
      541 cataatgccg ttgaggtgaa ctggattgac ccggataacg gctgggagac ggcgacagag
      601 cttgtggagg acacgcaggc cattgcccgt tactgtcgta acgtcacgaa gatggatgcc
      661 tttggctgta ccagccgggg gcaggcacac cgcgccgggc tgtggctgat taaaacggag
      721 ctgctggaaa cgcagaccgt ggacttcagc gtgggtgcgg aagggcttcg ccatgtaccg
      781 ggggatgtca ttgaaatctg cgatgatgac tatgcgggga tcagcatcgg cgggcgcgtg
      841 ctggcggtga acagccagac ccggacgctg acgctcgacc gtgaaatcac gctgccatcc
      901 tccggtacca cgctgataag cctggttgac ggaagtggca atccggtcag cgtggaggtc
      961 cagtccgtca ccgacggcgt gaaggtaaaa gtgagccgtg ttcctgacgg cgttgctgaa
     1021 tacagcgtat gggggctgaa gctgccgacg ctgcgccagc gactgttccg ctgcgtgagt
     1081 atccgtgaga acgacgacgg cacgtatgcc atcaccgccg tgcagcatgt gccggaaaaa
     1141 gaggccatcg tggataacgg ggcgcacttt gacggcgacc agagcggcac ggtgaatggt
     1201 gtcacgccgc cagcggtgca gcacctgact gccgaagtca ccgcagacag cggggaatat
     1261 caggtgctgg cgcgctggga cacgccgaag gtggtgaagg gggtgagctt tatgcttcgc
     1321 ctgaccgtgg cagcggatga cggcagtgag cggctggtca gcacggcccg gacgacggaa
     1381 accacatacc gcttcacgca actggcgctg gggcggtaca cgctgacagt ccgggcggta
     1441 aatgcgtggg gactgcaggg cgatccggcg tcggtatcgt tccggattgc cgcaccggca
     1501 gcaccgtcga ggattgagct gacaccgggc tattttcaga taaccgccac gccgcatctt
     1561 gcgatttatg atccgacggt acagtttgag ttctggttct cggaaggttc cagaccaggt
     1621 acgctgaatg attttctcgg tgccatgacg gaagatgatg tcatgccgga ggcattgcgt
     1681 cgttttgagg caatggtgga agaagcggca cgcaacgctg aagccgcctc tcagagcgca
     1741 gcagcggcaa agaaatccga aactgcagcg gcatcatcga agaacgcggc gaaaacctca
     1801 gaaacgaatg cagctaacag cgcacaggcg gcagcgtcct cgcagactgc atcggcaaac
     1861 tccgcgacag cagccaaaaa atcagaaacc aatgcgaaaa acagtgagac agccgcaaag
     1921 acgagcgaaa ccaacgcaaa gtccagccag acggcagcga aaacaagcga aacgaatgcc
     1981 aaagccagtg aaactgcggc gaaaagcagt caggatgcag cagccgaaag cgagagtgct
     2041 gcagctggtt ctgcgacttc agcagctgga tcagcaactg ctgcggctaa cagccagaaa
     2101 gccgcgaaga cgagcgaaac taacgcaaag tccagccaga cggcagcgaa gaccagcgaa
     2161 acgaatgcca aagccagcga aactgcggcg aaaaacagtc aggatgcagc agcccaaagc
     2221 gagagtgccg cagctggttc tgcaagcgcg gcggctgctt ctgccactgc atcagccaac
     2281 agccaaaaag cagcaaaaac cagtgaaacc aatgcaaaga caagcgagac tgcagcggcg
     2341 aactcggcga aagcatcggc agcaagccag acagcagcta aagcaagtga agacgcagcc
     2401 agagagtatg caagtcaggc agcagagccg tataaatatg tcttacagcc actgcctgat
     2461 gtgtggatac cgtttaacga ttcactggat atgattacgg ggttttcgcc atcatataaa
     2521 aagattgtta ttggtgatga tgaaataacg atgcctggcg ataaggttgt aaagtttaaa
     2581 cgtgcatcga aagcaaccta tattaacaaa tctggtgtgc tgacagaggc tgccattgac
     2641 gagccacgat ttgaacgtga tggcctgctt attgaggggc aaagaacaaa ctacatgctc
     2701 aattcggaaa gccctgccag ttgggggcga tcgtcaaata tggatgtgcc cgaaacaggg
     2761 acggataatt ttggttttac ctatggaaag tttgtctgca acgattctct gattgggcaa
     2821 acctcagcca ttaatatggc atcaattgct gcaacaaagt cagttgatgt ctcaggtgat
     2881 aataaacacg tgacaacctc atgtcgtttt aaaacagaac tgcaggtaag gttgcgtatc
     2941 cggtttgata aatatgacgg tagcgcaaca acttttcttg gtgatgcgta tattgataca
     3001 caaacgcttg aaattaatat gacaggcggt gctgcctcaa ggattacagc gagagtcaga
     3061 aaggacgaag ctaccggatg gatttttgca gaggcaacaa ttcaggcaat tgatggggag
     3121 ttaaaaatag gttctcagat acagtattct cctaagcagg acggggcaac cgtatctggt
     3181 gactatattt atctggccac cccacaagta gaaaatgggc cttgtgtatc atcttttatt
     3241 atatcaggaa cgacggcggc gacccgcgca agcgatatag tcacagttcc cattaagaat
     3301 aatctttata atcttccttt tacggttctt tgtgaggtac ataagaactg gtataaaacg
     3361 ccaaatgcag cgccgcgtgt ttttgatacc ggcggtcatc aaaccggagc ggctattatt
     3421 cttggcttcg gtcgttcaac agattacgac ggatttcctt attgcgatat tggaggagct
     3481 aacagacggg taaacgaaaa cgcatcgctt gaaaaaatgg ttatggggat gcgtgtaaag
     3541 tcagatcagt ctacgtgctc agtaagtaac gggcatatat ccagcgaaac aaaaaccaca
     3601 tggtcctgta ttcagaacac cgcaattatc cgtattggag gccagactac agccgggtta
     3661 cgtcatttat ttggtcatgt caggaatttc agaatatggc acaaggcatt gactgatgct
     3721 caggtggggg agtcaatcta atgaaagatt taacactcaa atttgccgac agggccgact
     3781 tttcggcctt tatggagagt attggctatt atgatgacga gtcgatgcag gatgatattc
     3841 ttattgacgt gataggtaac gtgtacaaag aaaccggaga actaactgaa gatggcgaac
     3901 cggtatgtgt taaggaagac ggatattttg taaacgtgcg catcattaat gatccgcaaa
     3961 tatcgtcatt attcgatgaa tacgtggttg ctgttgagca tcaacttcgt ggctggatgt
     4021 gaggaagaaa aatggctaca tcgacagtaa ttcctgatga catcaaaacg ctaaaatccg
     4081 acgttagcaa attaaaaaac gatcaaggaa gctacgcaac aaaattatat gtagacagca
     4141 aagatgaaat cgttggtgac tggtctgctt catggtatca gcaggtattg ccaactagcg
     4201 gagctatatt tgggagaaaa ctccgctcaa ctcacaggac ggcaggtgtt gaggatgcgt
     4261 attgcgaact atacctcaaa aaatggatag acagtccagg taacgcaatg gcgcgcctta
     4321 acctgaacga taacgggaca aacatttgct gggactttac caacctttat ggcggtacga
     4381 tgatttttcc cggtgacagc ggatacctca aaatgggtaa ctgccttatg tcatacagca
     4441 agcgtggaag taacgcgctt attaaatttg attacaccga cacattacag atcaaatatg
     4501 ccaatcatgg gtcaaccatg acattaaaca cacagggaac cgcttatgct ggtgttactg
     4561 ctcaattgtg gggcaactcc agccgtcctg ttgtttatga agtcggtgtt gatggtggcg
     4621 cttatatgtt ctatgcgcag aaaaataccg ataacaccta tatgttaagc gttaatggtg
     4681 catgtcatgc caccgcattt aaccagcatt ccgaccggga tctgaaagac aacattcagg
     4741 tgatcgataa tgcaaccgat cgcatccgta aaatgaacgg ctatacatac acgcttaaag
     4801 aaaacggtat gccctatgct ggtgtcattg cacaggaagc tctggaagca atcccagaag
     4861 ttgtaggttc cgcaatgaaa tatcaggacg gtgcaagcgg atcggaaggt gaagaaggtg
     4921 aacgttatta cacagtagat tattctggtg ttactggctt gcttgttcag gtagccagag
     4981 agtcagacga cagaataaca gcactggaag aagaaaacgc agaattaaga caaagattat
     5041 ctgcaattga ggcggcgctt gtgtctaaat aatattaagg ggccgagcgc cccgttttat
     5101 tgggtaggat gaaaatggat ataacacctt tccttcatgc gctttgtgct gtggctgcgc
     5161 agctactgat tggtcttttt accgggaact gggcttacgg ggcgatagcc ggttgtacgt
     5221 tcttcattgc gcgtgaacac acccaggcag aatatcgctg gattgaaatg ttcgggcatg
     5281 gcaagcgaat gaatatgccg tggtggggcg gttttgatcc gcgcgc
//
LOCUS       DADTFG010000217         5085 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.218, whole genome shotgun sequence.
ACCESSION   DADTFG010000217 DADTFG010000000
VERSION     DADTFG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.218"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..172)
                     /locus_tag="J1138_004393"
     CDS             complement(<1..172)
                     /locus_tag="J1138_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA8456087.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGCILRKL
                     CEWKSVRILEAEC"
     gene            complement(396..3617)
                     /gene="carB"
                     /locus_tag="J1138_004394"
     CDS             complement(396..3617)
                     /gene="carB"
                     /locus_tag="J1138_004394"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBA8456088.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLHAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3635..4783)
                     /locus_tag="J1138_004395"
     CDS             complement(3635..4783)
                     /locus_tag="J1138_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HBA8456089.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         1138 a         1407 c         1297 g         1243 t
ORIGIN      
        1 agcattcagc ttccagaatc cgtacacttt tccactcaca cagctttctc aaaatacagc
       61 ctattgctct acgcttctct ctgtagaaca cctgtcttcg gtattttggc gcaaatacta
      121 tgtgatattt acagttccat cgggtgtgcg ctaagctctt ttcgttcccc attgggaccc
      181 ccttttgatt tcttgtttga cacttgcagt tgccagaccg caaggtgttt taacaaatca
      241 aaaggggttt taataactgg ctcaaagctg aaagctttcc ggaaccccca gcctagctgg
      301 gggttttctg tgcacaaaaa aacggtcctc atcagagaac cgtattagtt attcgatcaa
      361 attagcggat gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc
      421 tgcaccgaaa ttactttttc ggtcgcatcg gcattcagcg ccatcgcggt ggcaaagccg
      481 ccgttcaggg tggtgtcgta atgcacttta tattgcagcg cactgcgacg gatcacgcgg
      541 gagtcttcaa tcgcacgacg acctgaggtg gtgttgatga tgtaggtata ttcgccattc
      601 ttaatacggt cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggattg
      661 atacccgctt cgcccagcac aatcgccgtg ccgtgagtcg catccagctc gaagccctgt
      721 ttcagcagtt ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc
      781 agcgcacgac cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct
      841 tcagcgaagg tgcggcccac gcccattact tccccggtag agcgcatttc tggccctaac
      901 agcgggtcaa cgcccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac
      961 ggcgggataa cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc
     1021 gccgccactt ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc
     1081 gcacgcgggt taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc
     1141 atcaggccgc gcacctgcag ttcgaaggcc agtttctgca cctgctggcg catcacatcc
     1201 tgaatttcct gacttaaggt gtaagctggc agtgaacatg cggagtcacc ggagtgcaca
     1261 cccgcctgct caatatgctc catgatgccg ccaatcagca ccatttcgcc gtcgcagatg
     1321 gcatccacgt caacttctac tgcgtcatca aggaagtggt ccagcaacac tggcgcatcg
     1381 ttagatacgc tgaccgctgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt
     1441 tccatcgccc gaccgccgag aacgtaagac ggacgcacca ccagcgggta gccaatctct
     1501 ttcgctttct caaccgccat ttcgatggtg gtaacggtgg cgttcgccgg ttgtttcagt
     1561 ttcagacgct caaccgcatg ctggaagcgt tcacggtctt ccgcacggtc gatggcatcc
     1621 gggctggtac cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg
     1681 gtctgaccgc cgtactggac gataacgcct ttcggcttct caatacgcac aatttccagc
     1741 acgtcttcca gagttaccgg ctcgaagtag agacggtcag aggtgtcgta gtcggtggag
     1801 acggtttccg ggttacagtt aaccataatg gtttcataac cgtcttcgcg cagcgccagc
     1861 gaggcgtgta cacagcagta gtcgaattcg ataccctgac cgatacggtt cgggccgccg
     1921 ccgagcacca tgattttttc acgatcggtg gacggattcg cttcgcactc ttcttcataa
     1981 gtggagtaca tgtaagcggt gtcggtggcg aactctgccg cacaagtatc cacgcgcttg
     2041 taaaccgggt gcagatcata ctggtcgcgc aacttgcgga tttccgcttc gcgcacgccc
     2101 gccagtttag ccagacgcgc atcggcaaag cctttgcgtt tcaactggcg caggaattca
     2161 gcgtgcaggc cagtgatgcc cacttctgcg actttctctt ccagacgcac cagctcttca
     2221 atctgtacca ggaaccagcg gtcaatgttg gtcaaattaa atacaccgtc tacagacagg
     2281 cccgcgcgga aggcatcagc gatataccag atacgctctg cgccagcatc tttcagttcg
     2341 cggcggattt tagtcagcgc ttccgggtcg tcgagactca ctttcggatc gaagccagtt
     2401 gcgcccactt ccaggccgcg cagcgctttt tgtagggatt cctgctgcgt gcgaccaatc
     2461 gccatcactt cgccaaccga tttcatctga gtggtcagac ggtcgttagc accggcgaat
     2521 ttctcgaagt tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag
     2581 gccggagtac gcccaccggt gatgtcgttc atcagctcgt cgagagtgta acccaccgcc
     2641 agtttcgccg ccactttggc aatcgggaaa ccagtggctt tcgacgccag cgcagaggag
     2701 cgagacacgc gtgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca
     2761 aactggacgt tggaaccacc ggtttcaacg ccgatttcac gcagtaccgc catcgaggcg
     2821 ttacgcatga tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag
     2881 tcaccggtgt ggatgcccat cgcatcaaag ttttcgatag agcagacgat gatgcagttg
     2941 tcgtttttat cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca
     3001 atcagcagct ctttggtcgg ggagagatcc aaaccgcggg cgcaaatttc ttcaaattct
     3061 tcacggttat aagcgatacc gccaccgcta ccgcccatgg taaaggatgg gcgaataatg
     3121 cacgggaagc caacttcagc ggcaaccgcc agcgcttctt ccattgaatg agcgataccg
     3181 gaacgcgcag tttccagacc gattttcttc atcgctacgt cgaaacggcg gcggtcttcg
     3241 gctttatcaa tcgcatcggc agtggcacca atcatggtga cgccgaactc ttccagcacg
     3301 ccctgacgct caagctccag cgcacagttc agcgccgtct gcccccccat ggttggcagc
     3361 accgcgtccg ggcgctcttt ttcaataatc ttgcgtacca cttcccagtg aatcggctcg
     3421 atgtaggttg catcagccat ttccgggtcg gtcatgatag tcgccgggtt ggagttcacc
     3481 agaatgacgc ggtaaccctc ttcacgcagg gctttacacg cttgcgcgcc agagtagtca
     3541 aactcacacg cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata
     3601 tctgtacgtt ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct
     3661 caattaacgc gataaagtgg tcaaacaacg gcgcggcgtc gtgtggacca gggctggctt
     3721 ccgggtgtcc ctggaagctg aacgccggtt tatcggtacg atgaatgccc tgtaacgtac
     3781 cgtcgaacag ggatttatgt gtgacacgca ggtttgcagg taatgttgct tcatccaccg
     3841 caaaaccgtg gttctgggcg gtgatcatca ccacattttt ctcaacatct ttaaccggat
     3901 ggttgccgcc gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca
     3961 gcagctgatg accgaggcag atgccgaata ccggaatatc agtttcgagg aatttctgga
     4021 tagcggtaat ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc
     4081 cgtccggatt cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc
     4141 gacagcctct atccaccagc atacgcagga tgttgcgctt ggcaccgaaa tcgtaggcca
     4201 cgacgtggta cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcagtgtcc
     4261 agctcccttg tgtccagcta tagggttctg cggtggttac ttcttttgcc agatccatgc
     4321 cgttcagacc tgggaacgcg cgggcttttt ctaacgccag cgccgcatcc gggttgtcac
     4381 ccgcaataat gcagccattc tgagcacctt tctcacgcag taaacgcgtc agcttacggg
     4441 tatcgatatc ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg
     4501 tattacggaa gttgctggca atcagcggca ggtcgcgaat caccagacct tgggcatgaa
     4561 cctgagaaga ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa
     4621 gagtaacgat ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg
     4681 aagtattgaa aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa
     4741 actgggttcc gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat
     4801 attcactcac tttatttgca tattaattca caatgatgac gtaaatcaat gcaaatctgc
     4861 ttaccgacga atttctggca aacggcggca ttctggagat atgggagcta aaagtcaact
     4921 taatggcggg attttttata ttattttgtg gctcatgcat gttttacagg cattaagcga
     4981 caaaaatatc atataacatg ttctggaaaa cgcttgcgca gcaagaagat tgtatgaaat
     5041 caaaatgagc agaaaaaagt ggaccaaatg gtcaaaattt acatt
//
LOCUS       DADTFG010000218         5081 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.219, whole genome shotgun sequence.
ACCESSION   DADTFG010000218 DADTFG010000000
VERSION     DADTFG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.219"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(104..1351)
                     /locus_tag="J1138_004396"
     CDS             complement(104..1351)
                     /locus_tag="J1138_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HBA8456090.1"
                     /translation="MTEKTERNKASSEEVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKTLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDDVPADLRERESTV
                     YRLRKTELAQSIAGLKQSKALLDKEIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRIRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVI
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKILPVIPGMIASVDIKT
                     GQKSVFQYLIKPITRMKQALQER"
     gene            complement(1412..3559)
                     /locus_tag="J1138_004397"
     CDS             complement(1412..3559)
                     /locus_tag="J1138_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HBA8456091.1"
                     /translation="MKSIFEGLSQITALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRSPEDIPSLAVPALLLLNAQEAIVVVRIEGAGLERQYHVLQPGGLR
                     KTLSHDALMAVYSGYCWFVQPQAMQDRRSELPEYDMSGAWFWRVLWRFRAYYGQVIVA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPSSSGSYANNLREYESVREFMTSATLLAFVDLP
                     FILLFISVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNGIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNEI
                     VRRPVERESQTHYLTVKDIQGEFRFSAVTFFYKPELPAALKGMSVCIRPGEKVGIIGT
                     IGCGKSTLLKMMVGLYRQNKGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPCGLDMQIGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTENIVLKGLNHWSRGRTMVVVTHRLQVLNIVDRIIV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            complement(3584..4828)
                     /locus_tag="J1138_004398"
     CDS             complement(3584..4828)
                     /locus_tag="J1138_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBA8456092.1"
                     /translation="MKRKLLLLLLFPFSTNSASLYSLIREAVMHDPIVMEARAELISA
                     QSRIEQASSAHWPVVTATGSKLLSQSHRYSYDYDSEDILPGIRGEVNIFASGAIEADV
                     HRSESEAEYYHYKMEETGEETIRSFVSLYLDALREKQSIEVLKQSLSRHNAILNDLNT
                     ISIHDTGRESEFVQAEARRLMVRQQINSRSRVLKTTLGKLSTWTKNPVTESDLENPFS
                     RMTEARLLTDFTQAPQKGNPSWLASRADVESKKAALKAQELARYPRVDLTGSVTRDDR
                     QIGVNLSWDLFNRNASYGVTEKAAQIVAATGRLDSVARMIDETGRLSLITVRQSRVEM
                     ETLRCQEQASARVVDFYRLQFRVARKTLIELLNAENELYSVGLSRVQTEDQMLHGMLD
                     YLYSQGMLLKWSGVNLSDEEEK"
     gene            complement(4887..>5081)
                     /locus_tag="J1138_004399"
     CDS             complement(4887..>5081)
                     /locus_tag="J1138_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016265937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBA8456093.1"
                     /translation="IELSGIRSDKIIMSLREHLVDGFSKREVCERNGVSLSYFSISLK
                     KISHIHNVCALISKYYNSNF"
BASE COUNT         1340 a         1366 c         1009 g         1366 t
ORIGIN      
        1 ttctccttaa atttaatcaa attatttgtt ttcattgcga tatgatacag acttcctgtt
       61 tagtgtctgt attacggagc catgaggcta ataatatgat aaatcagcgt tcctgaagag
      121 cttgtttcat tctggtgatt ggtttaatca ggtactgaaa tacacttttc tgacctgttt
      181 ttatatccac gctggctatc atgccaggaa taactggtaa tatttttccg ttcctgtctg
      241 tcagataatt attactggta gtaacgagta cccgataata tgcttcattt ggatccagtt
      301 ttaaatctcc cggccgtttc tggtcacgta aagtatccgg acttaccagt atcaccactc
      361 cgtcaagacc accataaatg gcatagtcat aagccgtcag ttttacaagt gccggcatcc
      421 ctgttctgac ataggcaaca tcccgtggat ttatataggc ttcaatcagc aactggtctt
      481 ccagcggaat aatttccatt atatcctgtc ctgcagaaac cacgcctccc aaagtattta
      541 cccgaatatt ttttacgatg cctctcaggg gagccctgat acgtgaacgc tcaaccgggt
      601 cggctctgcc agccatattc tcttttgcct gtgcaagctc tgcttctgtc ttcaccagtt
      661 cagctccagc ttccgtcagg tatttattct gtttctcatc catctgtaac tgcagctccg
      721 ctgactgacg ctgcatacgt aacagttcga cttccgacat cgccccttca cgcacaattg
      781 gccgggtcat tgctatttcc ttatccagca gagccttact ctgcttaagg ccggcaatgc
      841 tttgtgccag ttcagtcttt cgtagccgat aaactgtact ttccctctcc ctcaaatctg
      901 caggcacgtc atccggaaat gtcagtgatt cggaataagc ttctgcacgg agtcttgccc
      961 tgactgcttc caggttattc acccgggctt cactttccct cagcatggca gagcttcggg
     1021 tatcatccag tgtcagcagt acctgacctt tctcaacaat atcgccttcc ctgacctcaa
     1081 gtgttttcaa aatacccggg tcaagggtct gtataacctg ctccctgctg ccgggaataa
     1141 tactcccctg accacgggtc acttcatcca gaggactgtt ccatgcccag ataacaaacg
     1201 tcactaccag taccagcaga aaaatcacca tcaggaaaag ggcatgatgc ttttcctgct
     1261 gaagggctgc ctgtacatct gtcaacaggg ccaggtctga ctgcctgacc tcctctgacg
     1321 acgctttatt ccgttccgtt ttttctgtca tacccctgcg gtccacaaac tgttaaacat
     1381 atatgctctt tatttcttat ccggttgacg ctcatgaagt tatcgctctg ctctccccac
     1441 tctgctgttt ccctgacagt tttttcagta tttcatcgcg gggaccatcc atcattaccc
     1501 ggccttcatt catcacgata atacggtcaa caatattcag cacctgcaga cgatgcgtca
     1561 ccaccaccat tgtgcgtcca cggctccagt gattcagccc tttcagaaca atgttctctg
     1621 tctgcagatc aagacccgtc gtgggttcat caagcagaac tacccgggga gaacccagta
     1681 ccattctcgt cagagcgacc atctgacgct gtcctcctga cagcccctga cctccttccc
     1741 ctatctgcat atcaagcccg catgggtgat gacgaataaa acggtcaaac ccgaaacgtc
     1801 cggctgcatt aatcatatcc acatccgaca ggcttcccgg aagacgcgaa agctgcagat
     1861 tttcacgcag agttcccaga aaaaggcggg gattctgctc aagaaggaaa accttatccc
     1921 gaacaaaaca gggatccaac tgctgcatag caaccccgtc aagggtaatt tgtcctttgt
     1981 tttgacggta tagccccacc atcattttca gtagcgtcga tttaccacat ccgatagtgc
     2041 caataatgcc caccttttct ccgggccgga tacagacaga catacctttc aaggctgcag
     2101 gtaattcagg cttataaaaa aacgtgactg cgctgaaacg gaattccccc tggatatcct
     2161 tcactgtcag ataatgtgtc tgactttccc gttccaccgg gcggcgaact atttcattca
     2221 ccccttgcat agccagacgg gcctgctgga aacgggtggc cagtgatgct atctgaccaa
     2281 gcggtgccag cgcgcggccg gaaagaataa cggctgcaat cagtgccccc atggtgatac
     2341 ggttatgtgg gtcatcgctg tgaataaggt acgttcccag aatgaccagc agaacagtat
     2401 tcagttgctg gactgcagtg gaaaagcaga ttatgccatt actggtattt tttacccgaa
     2461 tggacgagcg ggcgacactg gctgtatatc catcccagcg tcgctgaacc cagttaacag
     2521 cattattcag cttcagggtt tctattccat ccagagcctc cacaagtaat ccctggcgct
     2581 gcgatgattc ccgcatggag gcattaatat gacgggaaag cggtcgctgc agtaatattc
     2641 ctgccagaat aaccagcgga accagcgtaa gaggtacaac cgcaagccag cctcccacga
     2701 gcccgataac actgatgaaa agcagaataa aaggcagatc cacaaaagcc agtaacgtag
     2761 cgctggtcat aaactccctg acagactcat attcccgcaa attgtttgcg tatgaacctg
     2821 aggatgacgg cctgtcagac atttttaccg ccatcacccg acgtagcaga gcagaactga
     2881 taatcaggtc tgccttctta cccgcaatat ccgtcagata actccgaatt aactttgcag
     2941 cacactcaaa cagaatggct gcaatcaccc cgatactcag catccataac gtttcccagg
     3001 aacgattagg tataacccgg tcatagacgt tcattacata cagggaactg accagcgcaa
     3061 gaaagttaat gataaaactc gcaacaataa cctgtccgta atatgccctg aatcgccaca
     3121 gtacccgcca gaaccatgcc ccgctcatgt catactctgg cagctccgag cgacgatcct
     3181 gcattgcctg tggctgaaca aaccaacaat aaccagaata aacagccatt aatgcatcgt
     3241 gagaaagtgt cttacggaga cctccgggct gaagcacatg atactgtctt tccagacctg
     3301 ccccctcaat acgcacaacc acaatggctt cctgagcgtt caataaaaga agcgccggaa
     3361 ccgcaagtga cgggatatct tccggagacc gccgggaaag atgattatca aatccttcac
     3421 tacgaagaaa ctcgccaaca gaacgaaaat caatcccgga tacatctgta cggacagtac
     3481 cggcagccag cgtctcagct gatacaggtg tacccagtaa cgccgttatc tgtgataatc
     3541 cttcaaaaat actcttcact taattaccag cccttcagcc ctgttatttt tcttcttcat
     3601 cagaaagatt cactccgctc catttcagga gcattccctg ggaatacaga taatccagca
     3661 taccgtggag catctgatcc tccgtctgaa cccgggagag tccgacactg tacagttcgt
     3721 tttcagcatt cagtaattca atcagtgttt ttcttgccac ccgaaactga agacgataaa
     3781 agtccacaac tctggctgaa gcctgttcct gacatctgag cgtttccatt tccacccgac
     3841 tttgtctgac cgttatcaga gataatcgcc cagtttcatc aatcattctg gcgacagagt
     3901 ccagtcgtcc ggtagctgcc actatttgcg cagctttttc cgtaacacca taactggcat
     3961 tacgattaaa caggtcccag gacagattga cacctatctg cctgtcatcc cgggttacag
     4021 atcccgtcaa atccacccga ggatatctgg caagctcttg cgctttcagt gctgcttttt
     4081 tactctccac atcagcccgg ctggcaagcc acgacgggtt acctttctga ggtgcctgtg
     4141 taaagtcagt taacaatctg gcctctgtca tccgggaaaa aggattttca agatcacttt
     4201 cggttaccgg attttttgtc caggtggaca gttttcccag cgtggtttta agtactctgc
     4261 tccgggaatt tatctgctgc cggaccatta accttctggc ttcagcctga acaaactcag
     4321 actcccgccc ggtatcatga atactgatag tattcagatc attaagaatt gcgttatgcc
     4381 gggagagact ctgtttaagt acttcaatgg attgtttttc cctgagtgca tcaagatata
     4441 atgaaacaaa cgagcgaatt gtctcctctc ctgtttcttc cattttataa tgataatatt
     4501 cagcttctga ttcactccga tgcacatccg cctcaatagc ccccgaagca aatatattca
     4561 cttcaccacg aataccgggt aaaatatctt cagagtcata atcgtaggaa taacggtgac
     4621 tttgtgaaag gagtttactt cctgtagctg tgacaactgg ccagtgtgca gagcttgcct
     4681 gttctatacg ggattgtgca gaaattaact ccgcccgtgc ttccattact atgggatcgt
     4741 gcataacagc ctccctaatt aaagagtaaa gagatgcact gttagtggaa aatggaaata
     4801 acaataacaa taaaagctta cgtttcattt cttatgattt ttattaaaaa cattgataga
     4861 taacgtgaat acctgtgttt tttacactaa aaattactat tatagtattt acttatcaat
     4921 gcacagacat tatgtatatg agatatcttc tttaaactaa tagaaaaata gctcagtgat
     4981 acaccgtttc tttcacatac ctctcgtttt gaaaaaccat ctaccaagtg ctctcttaat
     5041 gacataataa tcttatcact acgaatccca gacaattcta t
//
LOCUS       DADTFG010000219         5027 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.220, whole genome shotgun sequence.
ACCESSION   DADTFG010000219 DADTFG010000000
VERSION     DADTFG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.220"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..538
                     /locus_tag="J1138_004400"
     CDS             <1..538
                     /locus_tag="J1138_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456094.1"
                     /translation="DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITT
                     FFQTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQT
                     PSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISV
                     MFCLVKKMVDEYQQNSGQ"
     gene            complement(781..1476)
                     /locus_tag="J1138_004401"
     CDS             complement(781..1476)
                     /locus_tag="J1138_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBA8456095.1"
                     /translation="MDNRSKFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1469..2896)
                     /locus_tag="J1138_004402"
     CDS             complement(1469..2896)
                     /locus_tag="J1138_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBA8456096.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVVETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2907..3626)
                     /locus_tag="J1138_004403"
     CDS             complement(2907..3626)
                     /locus_tag="J1138_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBA8456097.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYTKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4139..4993)
                     /locus_tag="J1138_004404"
     CDS             complement(4139..4993)
                     /locus_tag="J1138_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA8456098.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTIRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1341 a         1311 c         1066 g         1309 t
ORIGIN      
        1 agataatata aaagtgagtt acagcctaaa aataaatcag acaacaccag gcatacttgt
       61 tagctgtgat agcaataata actttgcctg tcagaccaca atgactgaag atgttattca
      121 gcgaattact acattttttc agaccagccc agatgtcaag aacagagaaa taaagctgga
      181 atggtcagga gataagagag atttaccaac tgctgaagcg gaaatatctc gcgtgcaggc
      241 gtctatcatt aaatggtata cgtcagaata ccataatggc aggcaagttc tcgatgagat
      301 acaaacgcct tcagcaatta acagtgagct ttatacaaaa atgatatacc tgaccaggaa
      361 ctggtcactg tatccgaacg gtgatggctg tgtcactata agctcaccag aaataaaaaa
      421 taaataccct gctgccattt gcctggctct gggatttttt ctaagtattg tgatttctgt
      481 aatgttttgc cttgtcaaaa aaatggtaga tgaataccaa caaaactctg ggcagtaata
      541 aacagcattc tgacagagag tgcctgtata aaactatagt gacagtgacc gggaagggag
      601 cccgtcttca ggaatggatt cctgtctttt taactgtaaa tatcctggca ttattaatgt
      661 caggatattt acagaagagc agcataacca tgacggaacg gggttaagtc aggttgtcct
      721 taacgttatt cacaacctcg tataccgcct tcaggtttat gtccagacta catatctctc
      781 tcagcaatcc tcaataatca gatacaccgc tttcaccgga ccatgaaccc cgacgacttt
      841 gataagctca atgtccgccg ttgaactggg accactgatg atgttaatac atgacggcat
      901 tcgttcaccg gcctgcgctt tctgatgcaa tttttctgcg agttgcgcta cacgcggcag
      961 gattgtgctt ttacgcagga taaaaagaga agattccggg agcaggctca atgaacgccc
     1021 gcgcccggcg gcggaaaaaa gaaccacgcc tcccgattcg gttaaaccat attcagcata
     1081 tacgacgccc actttagcct gctctgcttg tgatatgttc tcaatacctt tcgccggatc
     1141 ccaaacaacg gcattgcatt cctgctgcaa acgttcgcta atccccaatt cctccagcct
     1201 cgtgtcacca ctaatcacga ccgactgatc tcccagctct ttacacagac gtattgcagc
     1261 ttctgccgcc ttcgcctcgc tggtcagttc acagcgcgtc aacataacat cgctggcaaa
     1321 ctggataaac gcgtcacagc gctgctgttg ggtaagttgg gtaagccgct cgttagcata
     1381 gttgttaagc ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgcc ccagtgcctg
     1441 agcaacgtta ttcaaaaatt tgctccgatt atccattctt tttctcctgc gcctgatgtt
     1501 tcttaaacca actacggaaa ctctctccgt cagcttcagg aagatcgcgt gcttccatcc
     1561 agtcgctaat cgcgccaaat ttgagtggtg ttttgccgcc attgataaac cagcttgccg
     1621 catgagcacc agccatcatc ccaactttcc acaatcctgg atgactattg gcataagcga
     1681 acatttttat cgcccgttgc tctgcttttg cggtgatccc tttttcagcc atcacccgac
     1741 gatgacgcaa aatcagtttt gacagcggaa tacgcaccgg acacacgctg tcacaagcgg
     1801 tgcataaaga gcaggcgtag ggtaaatctt taaaatcttt atagccgcca agtaacgggg
     1861 aaatcaccgc accaattggc cctggataaa tagagccata tccatgaccg ccaatatggc
     1921 gatatgccgg acaagtattc atacaagccc cgcaacgaat acagcgcagc acatcccgaa
     1981 attcagaggc cagcacctca gaacgcccgt tatcgacaat aaccagatga aactcttcag
     2041 gaccatcaac gtgccccgct tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac
     2101 gtgcaccaac ggcactgcgc gccagcatgg tgatcaatac atctacctct gcaaacgtgg
     2161 gagcaatacg ctccattccc atcactgcaa tatgcgtttt aggcagcgtg gtacacattc
     2221 gcgcattacc ttcattggtc accaggcata ccgaaccggt ctctaccacc gcgaaattac
     2281 agccggtaat acctatttca gcactgagga aatcttcgcg gattttttgc cgaatgaata
     2341 aggtcatcgc ttcaggcgtt tccggcccct catagcccag acgttcgtgt agcactcgac
     2401 ggatctgatg gcgatcttta tgaattgccg ggaccacaac atgagagggc ggatcttgat
     2461 ccagctggag aatgtattca cccagatcgg tttcaatcac ctgaatcccg gcatcctgca
     2521 acacatgatt gacaccaatc tcttcggtca ccatcgattt agatttcacc accttccggg
     2581 catttttgcg ttgggcaacc tgtaaaatgt agcgggtggc gtcttctttg gtttttgcaa
     2641 aatagacgtg acctccgttt tgcgtcactt tttctgagag ctggtacaga taagcgtcga
     2701 gattactcag aacatgatca cgtatctggg cggcccgatc gcgccactcc tcccagtgcc
     2761 ccaattcatc gaccattttt tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg
     2821 ctttacgcat gatcggatct tcaatttgct gacggatgcg tgtcttaaaa tctgtattac
     2881 tggttttgat cgacatcttt atatcctcag cggctcatca gcacttcagc aatatgcatc
     2941 actttgactt tctgcccttc ccgttgtaat cgtccactga tgttcagcag gcaactcacg
     3001 tcagcaccaa ttaaatactc agggcggact tccatcaggt gcgcaacctt ttctttcacc
     3061 atctcgccgg atatttcggc cattttgacc gagaacgtgc cgccaaatcc gcagcaggta
     3121 tcctgttcag caaaggtcaa cagctccagt ccacgcacat ttttcagcag cgtaagtggt
     3181 tcgtccttca cccccagctt acgggccagg ctacaagatg ggtgatacac cgctctccct
     3241 tgcaaactgg cacctacatc gactacccct aatgtattaa caataaaaga ggtgagatcc
     3301 tgcatacgcg cggcaacctt ttcggcacgt gatgcccatt caggttcatc cgccagatac
     3361 gtcgggtaac tttttacggc ataggtgcaa gagccagccg gtgaaataat gggatcgtcg
     3421 ttatcctcca gtgcggcgat cagatttttc atccctggaa tcgcttcttt ggtataacca
     3481 ctattgatcg caggctgacc gcagcatccc tgtttctccg ggaaatttac gcgacagccg
     3541 agtttttcca gtagcagcac ggagtctcgt gccattcttg atttcagggc gtcaccaata
     3601 caggtgacaa agaaattgac attcacaact aatactccat tacttcatgc ccatttatgc
     3661 ccgatggttc cccccttccg ggggatcaat catcaataaa tatggcgttc ttttttaatt
     3721 ggctagcaac ataccacccc tcatttgtat gacaaatgat aatctttcat ctaaacttca
     3781 aatgcaataa ctaattttat ttctttcggc atcgctcaca ttttatgggg aaatcgacga
     3841 tcatttttgg cataagaaat ctatttaaaa attgctacat cctttatacc tattcagggg
     3901 gtgttgagat atacactgat ggtgtacaaa caaaatcacc gacaataatc acgtatcaag
     3961 taatagtcgg gattttattt aattgaagaa atttagtttg catttttatt taaaaactga
     4021 gctagagtcc atcaaaaaaa tatataaaac ccaaaaccac cgttggaaat agaaatagtt
     4081 gaaaggctca caactcccgg aatgcgatta tctttgccct gatttctggc ggttttattc
     4141 agggtgcaag ctgtctgacc ctttcccgat attctcccgg agtacaacca aactcgcgga
     4201 caaacgcctt gtgaaaagat gattcgctgg catagcctac tgactcagcg atcaccacaa
     4261 cagggagcat ttcccgggaa aacatctggg ccgctatttg tagacgcaac tttgttaata
     4321 cagccagcgg cgtggttccg gaaacatcac ggaaaagctg ggcaaaactt gcccgggaca
     4381 tgtgggcgat gctggccagc gattcgacgg tccaggcgtg tccaggcatt tccatcattt
     4441 gctgtattac cgcaccaaga cgtggatgca gaagcaaact gagaatgttt ttctctgtat
     4501 taacctgtgc aatccactca cgcaccgcga gggtaaagaa tgtagcgcag atctggctac
     4561 acagagcatc cactcccggc attgccgatc tggattcctg ttgtaaaaac gggatcgcct
     4621 ccctcagcca gttatattcc acgctatggt tcactggtgc tacaaacagc gtttccggca
     4681 aagacgttaa aaaataacgc gccgaatgtt gcagtcgaat agtgccacag acaatacagg
     4741 tcgattcatt atcgacatga ctcaggcgat gagctgaatt ttgtggtagc aggaccacat
     4801 ttcccgggcg taatgtaaaa atctctcccg tcggcatttc cagcttcgcc gctccttgcg
     4861 ttaacgcatg ccaacgaata accgacaatt ccccggcacc atgtggaagt tgccagtcac
     4921 ttcctaacac gcaattctta tcgatcgttc cttgtggagc gttaagcatc aacaaacggc
     4981 taagagcatc catatcagac tcctgagtgg ttttttgatt acaaaca
//
LOCUS       DADTFG010000220         4987 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.221, whole genome shotgun sequence.
ACCESSION   DADTFG010000220 DADTFG010000000
VERSION     DADTFG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.221"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..1125)
                     /locus_tag="J1138_004405"
     CDS             complement(<1..1125)
                     /locus_tag="J1138_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA8456099.1"
                     /translation="MFRQEAFDHQKMKWRGRALLLPGIPFWLTAGLCLFFIIAFLTFM
                     IAGTYTRRVNVTGEVSTYPRAANVYSSVQGVVVKQFVTEGQVITAGAPIYQIDVSKST
                     RSGVVSDNQQRDIDGQLARIAQIISRLESSKQATLTMLEKQKAQYTAAFSRSTDILRR
                     AQEGIRIMKENMENYRHYQTKGLINKDQLTNQVALYYQQQNNLLGLSGQNEQNALQIT
                     VLESQIHTQAANFDNQIYQMELQRYELQKERLNIDAGGAIIVRALADGCIDSLSVTVG
                     QMVNVGDSLLQVIPHNIDHYALVLWVPNDAIPYIAAGDKVNVRYDAFPAENFGQFAGT
                     VSVISKAPASPQEMLTYQGAPKAALTAAVPYYKVIVIPEKQ"
     gene            complement(1314..1913)
                     /locus_tag="J1138_004406"
     CDS             complement(1314..1913)
                     /locus_tag="J1138_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012668025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8456100.1"
                     /translation="MNVFCGRWLLALLMGVTGASAADPIMPPGQESSPMPTLPPDTRC
                     SLSVSNPVVDYGMMSRWQMEDMGAGNVSPGTRSLMVSVVCLYSRTMRLRVEGEGSELG
                     GLRYGERGATRLRLLDAQLDGNAVELRTITPAGVITESGGHILALNAGQRLAPVMQGR
                     LAEGKALTARLEIQPVLAEGDAHVGSRQRSETMLTLTLE"
     gene            complement(1923..4358)
                     /locus_tag="J1138_004407"
     CDS             complement(1923..4358)
                     /locus_tag="J1138_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012441389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBA8456101.1"
                     /translation="MLLSIGHCQRQPLRHLLLLLIVILCTAPTWAAEETHSRDNLVFD
                     PDTLSERGMDPTIAALFGQAPRFLPGENTVALFVNGNPRGRVRVRFDERGALCADPAF
                     LQAAGLEPPPGHTDKAACFDLRCAWPQAEIYLEPGEVKVILVVPPEAFRASVPEGGNW
                     QHGGAAGMINYDAQYLNSSGDGGGLNYGQLGTEAGFNAGDWVVRSRQTFTRLNGEGHI
                     NHQAAYAQRSFVGIKKVLQAGQVSLSNSLFGTGQVLGFQLFPESALMSGQSGAGLVEG
                     VAQSQSVVEVRQSGVLVYSTAVPAGPFRLQGFSLLNTRTDLMVTLTDSNGEMRQFIVP
                     AATLLARAPLISPGLSFGAGKLEQQYGKAPLLGTVASGWQLTPFTSLNAGVLGAEPYR
                     AGALNLDTQLFDPTRLSLQSTLAQDTDHSNTGTLLSALLSHNLSGRLSISVNGRQQTA
                     GYRELSDALQRADRDNPNSQSRSRYQWGSGVNWSAEGLGNLSLSWVHSTTFAGNNTDY
                     LRGGWSRQFGRTYLGVSLEHNTGTLSGQADDRLYVSFSMPLGDGSNINSYLNTAKRSA
                     RAGVRYSDRTSQDRGWSIATDRDLRNNRTSVSGNFDFVTPVSQLNASVSHDNDNYTSW
                     STRASGAVVLHENGVTLSPYRVADTFGIARVGKESGVKLDTPAGPVWTDWRGYAVIPT
                     LNGYRRSAIQVDTRSLAKNIDIGNAWQESEVARGAIGRLDFDVLRTRRVLVGAAMADG
                     ERLPHGASVFNAAEELVTVVGDDGTVFVPDATPGMMLKVQSSGKTVCAMKLDLPETAE
                     TTGLYENATATCR"
     gene            complement(4375..4983)
                     /locus_tag="J1138_004408"
     CDS             complement(4375..4983)
                     /locus_tag="J1138_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA8456102.1"
                     /translation="MVEEGDGEGAINLQNTDSFPVLLLTTLQNIEQDTEKLLVVTPPV
                     ARVEPGKTQRVRFMLTAKTPLKTERLKRVVFEGVPPQEKNKNLVRMTVRQNLPVIIRP
                     ANLTRDEAPWKRLIWSLKAGVLNVNNPSPYVVRLGLGVTTLPDKRIWMLPNAYVLPGQ
                     QLMLTHQAKFGDTSVETASKVRISPATTWGFTVDSYEAPLAP"
BASE COUNT          990 a         1492 c         1406 g         1099 t
ORIGIN      
        1 ctgtttttcc ggaatgacaa tcaccttgta gtagggcaca gcggcggtca gcgccgcttt
       61 tggggctccc tgataggtca gcatttcctg cggtgaggcc ggggctttgg agatgacgga
      121 caccgtaccg gcaaactggc cgaaattttc cgccggaaag gcgtcataac gtacattgac
      181 cttgtcaccg gcagcaatgt aggggatggc atcgttaggt acccacagca cgagggcgta
      241 gtgatcgatg ttgtggggga tcacctgcag cagactgtca cccacgttga ccatctgacc
      301 aaccgtgacg ctcagcgaat cgatgcagcc atcggccagt gcacgaacga tgatcgctcc
      361 accggcatcg atgttgagcc gctccttctg cagctcgtag cgctgcagct ccatctggta
      421 gatctggttg tcgaagttgg cggcctgggt atggatctgg ctttccagca cggtgatctg
      481 cagtgcattc tgttcgttct ggccggacag gcccagcagg ttgttctgct gctggtaata
      541 cagcgccacc tggttggtga gttgatcctt gttgatcagt cccttggtct gatagtgccg
      601 gtagttctcc atgttctcct tcatgatgcg tatcccttcc tgagcacgcc gaaggatgtc
      661 ggttgagcgc gagaacgcgg cggtatattg cgccttctgt ttttccagca tagtgagcgt
      721 ggcctgcttg ctgctttcca gccggctgat aatttgcgca atgcgtgcca gttggccatc
      781 aatatcccgc tgctggttat cgctgacgac accgctgcgt gtgctcttgc tgacgtcaat
      841 ctggtaaatg ggcgctccgg cggtgatcac ctgcccttcg gtgacaaatt gtttgacgac
      901 gaccccctgg acggaggaat agacgttggc ggcacgcgga taggtactga cctcaccagt
      961 cacgttgacc cgccgggtat aggtaccggc gatcatgaag gtaagaaacg cgatgataaa
     1021 aaacaggcag agccctgccg tgagccaaaa gggaataccg ggtaagagca atgccctacc
     1081 tcgccatttc attttctggt gatcgaacgc ttcttggcga aacactgatg gttttcctta
     1141 tgatgcatta ttgggtacga ttttttcacg cccctgaggt tcatatgatc actcctgtct
     1201 ttggaaattc ttgctgtgac ctaattaaca ttcatggtta tttgatttgt ggtgcaacgt
     1261 agaccaaagc accaaggaaa gttgattggc tcacttcggc cggcgctgta tgcttactcc
     1321 agtgtcaacg tcagcattgt ttcagagcgc tgccggctgc cgacatgtgc atctccttcc
     1381 gccagtactg gttgtatctc cagccgtgcc gtcagcgcct tgccttcagc cagccttccc
     1441 tgcatcaccg gtgccagccg ctgtcctgcg ttcaaagcca atatgtgccc tccactctcc
     1501 gtaatgacac cggcgggtgt tattgtgcgc agctccaccg cattgccatc caactgcgca
     1561 tccagcaggc gcaggcgggt agcaccacgc tcaccgtaac gtagtccgcc cagttcactg
     1621 ccctcacctt cgacccgcaa tctcattgtc cgggaataga ggcagaccac gctcaccatc
     1681 agtgagcgtg tacccggaga aacattgccg gcccccatat cttccatctg ccagcgcgac
     1741 atcatcccgt aatcgaccac cggattgctg accgatagac tacagcgcgt atccggcggc
     1801 aatgtcggca tcgggcttga ttcctggccc gggggcataa tggggtcggc agcactggcg
     1861 cctgtgacgc ccatcaacag ggccagtaac caccgtccac aaaacacgtt catcgtttct
     1921 ccttaccggc aggtcgccgt ggcattttca tacaggcccg ttgtttccgc cgtctccggc
     1981 aggtcgagtt tcatcgcgca cacagtcttg ccggaactct gcactttaag catcatccct
     2041 ggcgtcgcat ccggcacgaa gaccgtgccg tcatccccta ctaccgtcac caactcctct
     2101 gcggcgttga acacgctggc gccatgcggc agacgttcgc catcagccat tgccgccccg
     2161 accagcacgc gtcgggtgcg aagcacatca aagtccagtc ggccgatggc tccccgagcc
     2221 acctcgctct cctgccaggc gttgccgatg tcgatatttt ttgccaagct gcgggtatcg
     2281 acctgaatgg ccgaacgccg gtagccattc agcgtcggta tcaccgcata tccccgccag
     2341 tcggtccata ccggccccgc gggtgtatcc agcttgactc ccgactcttt ccccactcgg
     2401 gcaatgccaa aggtatccgc tacgcggtag ggggaaaggg tgacgccgtt ttcatgcagc
     2461 acgaccgcgc cactggcgcg cgtcgaccaa ctggtgtaat tgtcgttgtc gtgacttacg
     2521 ctggcgttca gctgactaac tggcgtcacg aagtcaaagt tgccactgac cgatgtgcgg
     2581 ttattgcgca ggtcacggtc ggtggcaata ctccagcccc ggtcctgact ggtacggtca
     2641 ctgtagcgaa cgccggcacg tgcgctgcgt ttggcggtgt tcaggtaact gttgatgtta
     2701 cttccgtcgc ccagcggcat gctgaatgac acatacagac ggtcatccgc ctggccactg
     2761 agggttcccg tgttgtgttc gagactgacg cccagatagg tgcggccaaa ctgccgactc
     2821 cagccgccac gcaggtaatc ggtgttgttg ccggcaaaag tggtgctgtg tacccacgag
     2881 agagacaggt tgcccagccc ctccgccgac cagttgacac cactgcccca ctggtaacgg
     2941 cttcggctct ggctgttagg gttgtctctg tcggcccgtt gcaaggcgtc actcagttca
     3001 cgataccccg ctgtttgctg acgcccgttg acactgatgc tcaaacgtcc actcaagttg
     3061 tggctaagca gggccgacaa caatgtgccg gtattactgt ggtcagtatc ctgcgccagg
     3121 gtgctttgca ggctgagacg ggttgggtca aacaactgcg tatccaggtt cagtgccccg
     3181 gcacgataag gttcagcccc caatacaccg gcattcagac tggtgaacgg tgtgagctgc
     3241 cagccggagg ccactgtacc cagcagcgga gctttgccgt attgctgttc gagcttgcca
     3301 gcgccgaacg acagtcccgg cgagatcagt ggcgcacgtg ccagcaaagt ggcggcaggc
     3361 acaatgaact gtcgcatttc gccgttgctg tcggtcagag tgaccatcag gtcagtacgg
     3421 gtgttcagca gggaaaaacc ctgtaacctg aaagggccag ccggtactgc agtactataa
     3481 accagcacgc ctgactgacg tacctcgacc acggactgcg actgtgcgac cccctcgacc
     3541 agcccggctc cgctctgacc ggacatcaac gccgactcgg gaaacagttg aaaacccaag
     3601 acctgtccgg taccaaacag ggagttggag agactgactt ggcctgcctg caacaccttt
     3661 ttaatgccga cgaaactgcg ctgcgcatag gcggcctggt gattgatgtg gccttcgcca
     3721 ttgaggcggg taaaggtctg gcgactgcga actacccagt caccggcatt gaacccggct
     3781 tcggtaccga gttgaccata gttgagcccc ccaccgtcgc cggatgagtt cagatactgg
     3841 gcatcataat tgatcattcc ggccgcgcca ccgtgctgcc agttgccgcc ttcaggaact
     3901 gacgcacgga acgcttcagg cggaacgacc aggataacct tgacttcacc cggctcgaga
     3961 tatatctcgg cctgtggcca ggcgcaccgc aggtcaaaac aggcggcttt gtcagtatgt
     4021 ccgggggggg gctccagccc agctgcctgc agaaaggcag ggtcggcgca cagcgccccc
     4081 ctctcatcga atcgcacccg tacccgcccg cgaggattgc cgttgacgaa gagcgccacg
     4141 gtattttcac cgggcagaaa gcgcggagcc tggccaaaaa gagccgcaat ggtaggatcc
     4201 atgccgcgct cgctcaacgt atcgggatcg aaaaccaggt tatcgcggct gtgagtctcc
     4261 tctgccgccc aagtaggggc ggtacagagt atgacaatca gcagtaagag caaatgacga
     4321 agcggctggc gctgacaatg acctatcgag agcaacatcc tgtccacctt ctatttacgg
     4381 cgccagcggc gcttcatagc tgtcgacggt aaaaccccag gtggtggccg gggagatgcg
     4441 tactttactg gctgtttcga cgctggtatc accgaatttc gcctgatggg tcaacatcag
     4501 ttgctggccc ggcagcacat aagcgttcgg cagcatccag atcctcttgt caggcagcgt
     4561 ggtcaccccc agccccagac gcacgacata cggcgacggg ttattgacgt tcaagactcc
     4621 ggccttgagc gaccatatca ggcgtttcca aggtgcttca tcgcgggtca ggttggcagg
     4681 gcgaataatg accggtaggt tctgccgcac cgtcatgcgt actaagttct tgtttttctc
     4741 ctgcggcggc acgccttcaa acactactcg cttgaggcgt tctgttttta gcggtgtttt
     4801 ggcagtcagc ataaagcgta cgcgctgggt cttgccgggt tcgacgcggg caaccggcgg
     4861 cgtcacgacc agcagttttt cggtgtcctg ctcgatattc tggagcgtgg tgagcagcag
     4921 cacgggaaag ctgtcggtgt tttgcaggtt gatggcacct tcgccatcgc cttcctcaac
     4981 gataacc
//
LOCUS       DADTFG010000221         4960 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.222, whole genome shotgun sequence.
ACCESSION   DADTFG010000221 DADTFG010000000
VERSION     DADTFG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.222"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(3..461)
                     /locus_tag="J1138_004409"
     CDS             complement(3..461)
                     /locus_tag="J1138_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBA8456103.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLKVQRSRG"
     gene            581..1390
                     /gene="proC"
                     /locus_tag="J1138_004410"
     CDS             581..1390
                     /gene="proC"
                     /locus_tag="J1138_004410"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBA8456104.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHEQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSITPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1407..2522)
                     /gene="adrA"
                     /locus_tag="J1138_004411"
                     /gene_synonym="dgcC"
     CDS             complement(1407..2522)
                     /gene="adrA"
                     /locus_tag="J1138_004411"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBA8456105.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKNINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2624..2944)
                     /gene="psiF"
                     /locus_tag="J1138_004412"
     CDS             complement(2624..2944)
                     /gene="psiF"
                     /locus_tag="J1138_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBA8456106.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKIYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3063..4478)
                     /gene="phoA"
                     /locus_tag="J1138_004413"
     CDS             complement(3063..4478)
                     /gene="phoA"
                     /locus_tag="J1138_004413"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBA8456107.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4579..4839)
                     /gene="iraP"
                     /locus_tag="J1138_004414"
     CDS             complement(4579..4839)
                     /gene="iraP"
                     /locus_tag="J1138_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBA8456108.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT         1159 a         1340 c         1212 g         1249 t
ORIGIN      
        1 atttagccac gactacgttg cactttcagc caccacttct ccagctccgc agcaaaggcc
       61 tggcggtcac gttgtgaaag actatctggt cctccggtct ggatcccact ggcacgtaag
      121 gtatccataa aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt gtaacgttcg
      181 ccgcgcggat taagcgccgc cgcgcctttc tcgatggctt cagcagccaa aggtatatct
      241 gcggtgatca ccaaatcgcc cgcttcacac tgccgaacaa tttcgttatc ggcaacgtcg
      301 aaacctgccg cgacgcgcag cgtacgaata aatcgcgatg gcggcacgcg taaactctgg
      361 tttgctacca gtaccagcgg catctgcata cgttccgccg cgcgatacaa aatctcttta
      421 attacattgg gacacgcgtc ggcatccacc caaattgtca taaagtcatc ctttgttggg
      481 taatcctcta ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg cttctgccag
      541 cgattatcaa aacaatgaat ttcacggcag gagtgaggca atggaaaaga aaatcggttt
      601 tattggctgc ggcaatatgg gaaaagccat tctcggcggt ctgattgcca gcggtcaggt
      661 gcttccaggg caaatctggg tatatacccc ctccccggat aaagtcaccg ccctgcatga
      721 acagttcggc atcaacgccg cagaatcggc gcaagaagta gcgcaaatcg ccgacatcat
      781 ttttgccgcc gtaaaaccag gcatcatgat taaagtgctt agcgaaatca cctccagcct
      841 gaataaagac tctctggtcg tttctattgc tgcaggtatc acgctcgacc agcttgcccg
      901 cgcgctgggc catgaccgga aaattatccg cgccatgccg aacactcccg cgctggttaa
      961 tgccggtatg acctccataa cgccaaacgc gctggtaacc ccagaagata ccgctgatgt
     1021 gctgaatatt ttccgctgct ttggcgaagc ggaagtgatt gctgagccga tgatccaccc
     1081 ggtggtcggt gtgagcggtt cttcgccagc ctacgtcttt atgtttatcg aagcgatggc
     1141 cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg tataaatttg ccgctcaggc
     1201 ggtaatgggc tccgcaaaaa tggtgctgga aacgggagaa catccgggaa cactgaaaga
     1261 tatggtctgc tcaccgggag gcaccaccat tgaggcggta cgcgtactgg aagagaaagg
     1321 cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag aaaaactcag
     1381 caaatcctga tggcttttgc cggacgtcag gccgccactt cggtgcggtt acgtccggct
     1441 ttctttgctt tgtagagcgc caaatctgcc gatttcaacc actcacgata gtgactcatt
     1501 tgtggattca gcggcgcaac ccccacacta atccgtaaag ttacctgtgg cgtattcggc
     1561 aagcgtaacg tgtttagccc ttcatgcacc cgtaacatgg cggtgatggc gctctcagct
     1621 ggcgtaccgg acatgattac tgcaaactca tcgccgccaa accgaccaat cacatcgcta
     1681 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc acccacatca
     1741 tggccccagg tatcgttgat gttcttgaaa tggtcgatat cgataatcag taacgttgca
     1801 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
     1861 cggttataca cgcccgtcat accgtcgcgg gtactcatga cctgcaacct gcgtttatgt
     1921 tccgccagtt tagtcgccgt ctggtagctg acccagccaa acagcagagg ataaatgaca
     1981 ataatgggaa gggagagcca ccattccagc ggtgcgctat tgaacgacac ggtgatgccc
     2041 gtcagctcga gggtgacaag gcaggagacc accatcaaca ccagacccgc gacaaacaga
     2101 cggggaccgc ctgcccccat caaattcaga cacataatca tcaacatcgc ggtggaaggc
     2161 agtgcgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
     2221 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatttgcca ggctaaatgc
     2281 ggccagacga acgcccagcc gaccaacaac agccaccacc agcccggcga cggatgcgaa
     2341 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcgcg gggtagtctg
     2401 acgcgacggg caaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
     2461 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
     2521 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
     2581 gggtgagcca ttttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
     2641 ggcaggcact cataaactta ttacgatcat cccctttcag agattgttgt gttgcttgat
     2701 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagatt
     2761 tgctgttctt caggcaatca ctcatgtaga tcttacgagc atcccctttc aacgcctgcg
     2821 ccgtcgcctg ctgattacaa gaggtcatac gctgttgttg tggggttaag gttctctcgg
     2881 cagcgccgac ggtggttaaa aaaaccagac cgaaaagcaa ggtaaccagt aatgtaattt
     2941 tcatcgcacc atccctcttc atgttttaac catgagcgta tgcgcccgtg atctgtcatt
     3001 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     3061 ttttatttca gccccagggc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     3121 ccaacaacat tggcggcatg cgggccatac gccgcaatac gcaactgact gccggtatgt
     3181 tcttgtgaat cctcttcgga gttcccgtaa ctcatcacca tcactgcgcc atctttggta
     3241 tttagcgcct gggtgaggcc cggagctttg gtatccggcg caactatctg gctggcatgg
     3301 gcgtgatcag cagtgactat gaccaacgtg ttaccatcct ttttagcgaa ttccagcgcc
     3361 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     3421 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaagccttt ctcattttta
     3481 ctcaacaatt caatggcttt gtcggtcatc tgcgccaggg tcggtacact gtcattacgt
     3541 tgcggattag gcgtacaggt gactgcgggc ttatctatat taccgtggta cgttgctttc
     3601 ggtccttgcc agcgcactgg catattgccg tcagcaaaca gtcctaatag gggtttttgc
     3661 tgattcgctt ccgtcaccga attcagtgag gcagcatcgc tcaccaactg ataaccacgc
     3721 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cggcggttgc cgtttcagca
     3781 aaggtttttg cgccgccgcc aagcgtaaca tcggcacggg cgttaagcag ctgttcggta
     3841 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcactc
     3901 ggaccgtagc atttgcgcga ggtcacatgc gccaccagcg cagcgggcgt ggcatcctgc
     3961 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     4021 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacacca
     4081 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccggttttt
     4141 ttattcagcg catagtgagt gtattgcccg gtaagcggta atgcatcgat acctttaaaa
     4201 aagccgcccg caccttcggc ataattgcgt gcggcagtaa tttccgagtc ccccatccca
     4261 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     4321 gcggtctgat cacccgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     4381 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     4441 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     4501 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     4561 gttgaaaaga ttagcttttt actgacgagg atgcctcaat aacttcttta cgtaatcgcg
     4621 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     4681 ctctacctga tcaatcaacc gctgttgatc attttgcgcc atattgcgaa gcattgccgt
     4741 gacgataatc tccaaagctt ctacctgcgc gcacagttct ttcgactctt cttctttttg
     4801 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     4861 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     4921 gacaaaggaa atactttgcg caaatatttt ttgtttgatt
//
LOCUS       DADTFG010000222         4857 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.223, whole genome shotgun sequence.
ACCESSION   DADTFG010000222 DADTFG010000000
VERSION     DADTFG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.223"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            3..359
                     /locus_tag="J1138_004415"
     CDS             3..359
                     /locus_tag="J1138_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBA8456109.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            459..1673
                     /locus_tag="J1138_004416"
     CDS             459..1673
                     /locus_tag="J1138_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8456110.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKTTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            1900..3165
                     /locus_tag="J1138_004417"
     CDS             1900..3165
                     /locus_tag="J1138_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8456111.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            3177..4043
                     /gene="ydiB"
                     /locus_tag="J1138_004418"
     CDS             3177..4043
                     /gene="ydiB"
                     /locus_tag="J1138_004418"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBA8456112.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            4074..4832
                     /gene="aroD"
                     /locus_tag="J1138_004419"
     CDS             4074..4832
                     /gene="aroD"
                     /locus_tag="J1138_004419"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBA8456113.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT         1234 a         1107 c         1130 g         1386 t
ORIGIN      
        1 cgatgaacgc ttatgaactc caggcacttc gccatatttt tgccatgact attgatgaat
       61 gcgcgacctg gattgcccag accggtgaca gtgaaagctg gcgtcagtgg gaaaatggca
      121 aatgcgccat tcctgatcgt gtggtcgagc aactgttggc tatgcgacag caacgaaaaa
      181 aacatcttca tgccatcata gagaaaataa ataaccgcat cggcaacaac accatgcgtt
      241 ttttccccga cttaaccgtg tttcaacaag tctaccctga tggcaatttt attgactgga
      301 agatctatca atcggtagcc gctgaattat atgcacacga tctggagcgg ctttgttaat
      361 ttccccacag aaaggaattg tcgttgttac aacaataatg aacggatact gacacaacat
      421 cgctacactt tttaaagcac ctttgctaag tagaacctat gaaaaatccc tattacccta
      481 ccgcactggg gttgtatttt aattatctgg tgcatggtat gggcgtcatt ttgatgagcc
      541 tgaatatggc ctcgctggag acgctttggc agactaatgc cgcgggcgtc tcgatagtta
      601 tctcatcact gggcattggt cgattaagtg tcttgctttt tgcaggatta ttatccgatc
      661 gctttggtcg ccgccctttt atcatgctcg ggatgtgctg ctatatggcc ttcttttttg
      721 gcatcctgca caccaataac atcattatcg cttatgtttt tggctttctg gcgggaatgg
      781 caaacagttt tctcgatgca ggcacctatc ccagtttgat ggaagctttt ccacgctcac
      841 cgggtacagc caatatttta attaaagcat ttgtttccag cggacaattt ttattaccgc
      901 taatcatcag cctgttagtg tgggctgaac tgtggttcgg ttggtccttt atgattgccg
      961 caggcattat gtttattaac gctctgtttt tataccgttg tacgttccca ccccatccgg
     1021 gtcgtcactt acctgtcata aagacaacca ccagctctac ggaacatcgc tgttcaatta
     1081 tcgatttagc cagttattcc ctatatggct atatctcaat ggcaacgttt tatctggtta
     1141 gccagtggct ggcacaatac ggacagtttg tcgctggaat gtcatacact atgtcgatca
     1201 aactactcag tatttacacc gtaggttcgc tgctttgtgt atttattacc gctccactca
     1261 ttcgtaatac tgttcgccca acaacattac tgatgctgta cacctttatc tcatttatcg
     1321 ccctgctcac cgtctgcctg catcccacat tttatgtggt gataatattt gctttcgtca
     1381 tcggttttac ctccgcaggc ggtgttgtac aaattggcct gacgttaatg gcggaacgtt
     1441 ttccctacgc taaaggaaaa gcaacgggga tctattacag tgcgggcagc attgcaacat
     1501 ttaccattcc gttgattacg gctcatcttt cgcaaagaag tattgccgat attatgtggt
     1561 tcgataccgc catcgctgct atcggcttta tactggcact gtttattggc ttacgcagtc
     1621 gcaaagaaac gcggcatcac acgctaaagg aaaatatcgc tccgggtggg taatgcaata
     1681 ttcttttcag attatgcaag atcttacgga taaataactc tttctgcgct aactaaggaa
     1741 aatcgcgatc aaaaacaaac tatgacatgc aatattcctg gaaacataaa ctttatgcca
     1801 tgtacccagg gaaaatcatc ttcagtatag taattatgta aaccgtcgga gaacaatacg
     1861 tacggtaacg aaattatctt tcagcaagga gctgtgaaaa tgtctcaaaa taaggctttc
     1921 agcacgccat ttatcctggc tgttctttgt atttacttca gctatttcct gcacggtatt
     1981 agtgttatta cgcttgccca aaatatgaca tctctggcag aaaagttttc cactgacaac
     2041 gcgggcattg cctatttaat ttccggtatc ggtttggggc gattgatcag tattttattc
     2101 ttcggtgtga tctccgataa gtttggtcgt cgggcggtga tattaatggc agtaataatg
     2161 tatctgctat tcttctttgg tatccctgct tgcccgaatt taactctcgc ctactgtctg
     2221 gcagtgtgcg taggtatcgc taactcagcg ctggatacgg gtggctaccc cgcgctcatg
     2281 gaatgctttc cgaaagcctc tggttcggcg gtcatactgg ttaaagcgat ggtgtcattc
     2341 gggcaaatgt tctacccaat gctggtgagc tatatgttgc tcaataacat ctggtacggc
     2401 tatgggctga ttattccggg tattctgttt gtactgatca cactgatgct gttgaaaagc
     2461 aaattcccca gccagttggt ggacgccagc gtagctaatg aattaccgca aatgaacagc
     2521 aaaccgttag tctggctgga aggtgtttca tcagtactgt tcggtgtagc cgcattctcg
     2581 accttttatg tgattgtggt gtggatgccc aaatatgcga tggcttttgc tggtatgtca
     2641 gaagctgagg cattaaaaac catctcttat tacagtatgg gttcgctggt ctgtgtcttt
     2701 atttttgccg cactgctgaa aaaaatggtc cggcccatct gggctaatgt atttaactct
     2761 gcactggcaa caataacagc tgccattatc tacctgtacc cttctccatt ggtgtgtaat
     2821 gccggagcct ttgttatcgg tttctcagca gctggcggca ttttacagct cggcgtttcg
     2881 gtcatgtcag agtttttccc aaaaagcaaa gccaaagtca ccagtatata tatgatgatg
     2941 ggtggactgg ctaactttgt tattccgctg attaccggtt atctgtcgaa catcggcctg
     3001 caatatatca ttgttctcga ttttactttc gcgctgctgg ccctgattac cgcaattatt
     3061 gtttttatcc gctattaccg cgttttcatt attcctgaaa atgatgtgcg gtttggcgag
     3121 cataaattta gcacccggtt aaacacgatt aagcatagag gttaaaggag ttaattatgg
     3181 atgttaccgc aaaatacgaa ttgattgggt tgatggccta tcccatccgc cacagtttat
     3241 cgcccgaaat gcagaataaa gccttagaaa aagcgggatt gccatttacc tatatggcct
     3301 ttgaagtgga taacgatagt tttcccggag caattgaagg attaaaagcc ctcaaaatgc
     3361 gcggaactgg tgtatcaatg ccgaacaaac aactggcgtg tgaatatgtt gatgaattaa
     3421 cgccagcagc caaactggtg ggtgccatca acaccatcgt aaatgatgat ggctatctgc
     3481 gtggctataa caccgacggc acgggccata ttcgcgccat taaagagagc ggttttgata
     3541 tcaaaggcaa aacgatggtg ctgttagggg ccggaggtgc ctcaacggca attggcgcgc
     3601 agggggcaat tgaaggttta aaagaaatta aactctttaa ccgtcgggat gagttcttcg
     3661 ataaagccct cgccttcgcg cagcgggtta acgaaaacac cgattgcgtc gtgacagtca
     3721 ccgatctcgc cgatcagcaa gcctttgctg aagccctggc ttccgccgac attttaacca
     3781 atggcaccaa agtgggtatg aaaccccttg agaatgaatc attggttaat gatatcagtc
     3841 tgttacatcc gggacttctg gtcactgaat gcgtgtataa cccgcatatg acgaagttat
     3901 tgcagcaggc gcaacaagcg ggttgcaaaa cgattgatgg atacggcatg ttgttgtggc
     3961 aaggggctga acagttcacg ttatggactg gcaaagattt ccctctggaa tatgttaaac
     4021 aggtcatggg gttcggtgcc tgacaggctg accgcgtgca gaaagggtaa aaaatgaaaa
     4081 ccgtaactgt aaaagatctc gtcattggta cgggcgcacc taaaatcatc gtctcgctga
     4141 tggcgaaaga tatcgccagc gtgaaatccg aagctctagc ctatcgtgaa gcggactttg
     4201 atattctgga atggcgtgtg gaccactatg ccgacctctc caatgtggag tctgtcatgg
     4261 cggcagcaaa aattctccgt gaaaccatgc cagaaaaacc gctgctgttt accttccgca
     4321 gtgccaaaga aggcggcgag caggcgattt ccaccgaggc ttatattgca ctcaatcgtg
     4381 cagccatcga cagcggcctg gttgatatga tcgatctgga gttatttacc ggcgatgatc
     4441 aggttaaaga aaccgtcgcc tacgcccacg cgcatgatgt gaaagttgtc atgtccaacc
     4501 atgacttcca taaaacgccg gaagccgaag aaatcattgc ccgtctgcgc aaaatgcagt
     4561 ccttcgacgc cgatattcct aagattgcgc tgatgccgca aagtaccagc gatgtgctga
     4621 cgttgcttgc cgcgaccctg gagatgcagg agcagtatgc cgatcgtcca atcatcacga
     4681 tgtcgatggc aaaaactggc gtaatttctc gtctggctgg tgaagtattt ggctcggcgg
     4741 caacttttgg tgcggtaaaa aaagcctctg cgccagggca aatctcggta aatgatttgc
     4801 gtacggtatt aactatttta catcaggcat aagcaataat atttcggcgg gaatacc
//
LOCUS       DADTFG010000223         4849 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.224, whole genome shotgun sequence.
ACCESSION   DADTFG010000223 DADTFG010000000
VERSION     DADTFG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.224"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            154..1128
                     /gene="hemB"
                     /locus_tag="J1138_004420"
     CDS             154..1128
                     /gene="hemB"
                     /locus_tag="J1138_004420"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBA8456114.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1168..2019)
                     /gene="tauD"
                     /locus_tag="J1138_004421"
     CDS             complement(1168..2019)
                     /gene="tauD"
                     /locus_tag="J1138_004421"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBA8456115.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHHV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(2016..2843)
                     /gene="tauC"
                     /locus_tag="J1138_004422"
     CDS             complement(2016..2843)
                     /gene="tauC"
                     /locus_tag="J1138_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBA8456116.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2840..3607)
                     /gene="tauB"
                     /locus_tag="J1138_004423"
     CDS             complement(2840..3607)
                     /gene="tauB"
                     /locus_tag="J1138_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBA8456117.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQIQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3620..4582)
                     /gene="tauA"
                     /locus_tag="J1138_004424"
     CDS             complement(3620..4582)
                     /gene="tauA"
                     /locus_tag="J1138_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA8456118.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKLGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKGAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPM
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1135 a         1388 c         1223 g         1103 t
ORIGIN      
        1 cagtgaatat gaaattattg ctatttctat tacaaagatg tcctgacctc tttttcattc
       61 tctttttgtc aacatcgcga caactttcgt aaaacacacc cagcctgctt caggtatact
      121 atgctcctcg attccacaaa catcaggcag accatgacag atttaatcca acgccctcgt
      181 cgcctgcgca aatctcctgc gctgcgcgct atgtttgaag agacaacact tagccttaac
      241 gacctggtgt tgccgatctt tgttgaagaa gaaattgacg actacaaagc cgttgaagcc
      301 atgccaggcg tgatgcgcat tccagagaaa catctggcac gcgaaattga acgcatcgcc
      361 aacgccggta ttcgttccgt gatgactttt ggcatctctc accataccga tgaaaccggc
      421 agcgatgcct ggcgggaaga tggactggtg gcgcgtatgt cgcgcatctg caagcagacc
      481 gtgccagaaa tgatcgttat gtcagacacc tgcttctgtg aatacacatc tcacggtcac
      541 tgcggtgttc tgtgcgagca tggcgtcgac aacgacgcga ctctggaaaa tttaggcaag
      601 caagctgtgg ttgcagctgc tgcaggcgca gacttcatcg ccccttctgc cgcgatggat
      661 ggtcaggtac aggcgattcg ccaggcgctg gacgctgcgg gctttaaaga tacggcgatt
      721 atgtcgtatt cgaccaagtt cgcctcctcc ttttatggcc cgttccgtga agctgccgga
      781 agcgcattaa aaggcgaccg caaaagctat cagatgaacc caatgaaccg tcgtgaggcg
      841 attcgtgagt cgctgctgga tgaagcccag ggcgcagact gcctgatggt taaacctgcc
      901 ggagcatacc tcgacatcgt gcgtgagctg cgtgaacgta ctgaattgcc gattggcgcg
      961 tatcaggtga gcggtgagta cgcgatgatt aagttcgccg cgctggcggg tgctatagat
     1021 gaagagaaag tcgtgctcga aagcttaggt tcgattaagc gtgcgggtgc ggatctgatt
     1081 ttcagctact ttgcgctgga tttggctgag aagaagattc tgcgttaatg atcattatgc
     1141 cgggttatcc aacccggcat ttccccctta ccccgcccga taaaacggaa tatccccaag
     1201 aatcgtcgcc cgatgcataa tccgccgctg tggcaggtaa tccgcattgg catagtgctg
     1261 agtcacgcgg ttatcccaaa tcgcaatatc attcggttgc cagcgccagc gcacctgaaa
     1321 ctccggttta gtgatatggg caaacaaaaa acctaacaag gcttcgctct ctttctcgct
     1381 gacatcaaca attcgtgtgg taaaaccttc gttcacaaac agcgcctgtt taccggtcac
     1441 cggatgcgtt cgcaccaccg gatgtagcaa cggtggattt ttcgcgaccg cctcacgcca
     1501 gcgttgatgc tcagcctcgg ttttgcggta cttgtattcc gggaacgatt tacggaaatc
     1561 atgctccgca cgcaacccgc tcaacagctg gcggaaggga acagagagcg cctcatatgc
     1621 ggcaatacca ctggcccaga gcgtatcgcc gccggtcgag ggtaactctt tcgccgccag
     1681 aatcgcccct gcgggtggcg tttgaataaa tgtcacatcg gtgtgccagt tgtcgttatc
     1741 cggcggatta tcgttatggg tatccaggac gatgatctcg tcaacccctt cagcatgagg
     1801 gtaaacaggg tgaatatgca gctcgccaaa acgctgggcc agcgcgcgtt gctgctgcgg
     1861 cgtaatagct tgatcgcgca gaaacaccac atgatggcgc agcaccgcat ggtaaagctg
     1921 ttcaaactga ttatcgctta acgggcgcgt taagtcggcc cccgtaattt gtgcgccaat
     1981 atacggcccc agcggggtaa tgctcagacg ttcactcatt gtacttctcc atgccagggc
     2041 gtcaggcggc gctgtaacgc gcgcagaccc agttctaaaa gaaaggcgat aatcgcaatc
     2101 accgcgatcc ccgccagcac cacgtcagtc gcgagaaatt cacccgctga ctgaaccata
     2161 aatcctaaac cgcgcgttgc ggcaatcagc tccgccgcca ccagcgtaga ccagcccaca
     2221 cccagaccaa tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa aatgacaaac
     2281 cacagcacct gcgcacggct ggcacccagc gactgggcgg cacgaatgcg aacctgctgc
     2341 gcgcttttca cccccgccag cgccgacatc gccaccggcg caaaaatcgc tagatagatc
     2401 agtaagatct tcgaggtttc gccaatacca aaccagatca ccatcagcgg caaataagcc
     2461 agcggcggca ccggacgata aagctcgatt atcggatcca gaatgccgcg caccgtcggg
     2521 cttagcccca tggcgatccc gaccggaata ccgatcaaca ccgccgcgag tagcgccagc
     2581 acaatgcgcg tcaggctggc tgccagatgc tgccacagcg tggcgtccat aaatccctgc
     2641 ggcccggcaa tggtgagcag ttttgccagt acctgttgcg gtggcggcaa aaacagcggg
     2701 ctaatcagtt gcagcgccgc gactgcccac catacagtga gcaaaaccgc taacgtacca
     2761 atgcttaagg tcacctgacg cgagagcggc cagcgccatt tcagccgatg cgaatgcagt
     2821 ttttcattaa tgagcacact catgagaacg cctcccgttg ctcaaatacg cggcttaaaa
     2881 catattcgcg catggcgatg aattgtggat cggacttgat gctgcggctc gactctcccg
     2941 caacaaagcg gcgagcaaag ttgagcggca gccgctccag cacacggcct gggccgggtg
     3001 aaagcagaac cagttcagtc gccataaaca ccgcttcttc tatatcgtgg gtaatcaaca
     3061 gcacctgctt tcccgtctcc tgccagagtt tcagcagcag ggtttgtatc tggtcgcggg
     3121 tgaaggcgtc cagcgcacca aacggttcgt cgagtaataa cagctgggga tttgccgcca
     3181 acgcgcgggc aatccccacc cgctgacgtt gaccaccgga aagctgccag atgtagcgtt
     3241 tttctgcgcc ttccagcccg acttttttca ccatctggtg tgcgatttcc agtcgctgca
     3301 ttttctctac acctgccagt tgcaggccga acgccacgtt gtcctgtaca ttgcgccacg
     3361 gtaacagccc ttcattctga aaaactacgc cacgctctgc tcccggtccc tgaataccct
     3421 tacccgccag ttgaatgctg ccatgctgat aaggcacaaa accagcaatc aaattcagca
     3481 acgtagtttt gccgcagcca gacggcccca gaactaccag aagctcgccg ctttccagcg
     3541 tcaggttgat atcttccagt gccggtttgc cgccataatc ggcgtaaaga tgagaaattt
     3601 gcagcatctg cgcctccttt tattgcacga agcgcgaggt aacgtactgg ctgtaatcat
     3661 tcgctacagc cgggactttg ccctgctctt tcaaaaactg cgcggtgtcg atgatcgctt
     3721 tgttcaccgg tccggtcagt tctgccgttt gttgcatcgg cgtcagatag gtattcccct
     3781 tcaccagccc cggaacgtca ccttccggca cgccgcttaa ccgcgccagt ttgctgatgt
     3841 tttccggctg tttcagccac gcgtctgggt tagcaatgta cggttgctga gcatcgatgg
     3901 cgcctttagc gaacgctttc acgacctcag gatgtttctc ggcaaaatct ttccgcacga
     3961 cccatacgtc cagtgtcggc gcgccccact gcccgacctg ttcagaatcg gtcagcacct
     4021 tgccatcttt ttccagggcg ttaaccgccg gtgcccagac ataagcacca tcaatatctc
     4081 cccgctgcca tgcggcgata attgcgggcg gctgcaggtt cacaatctca acttgcccaa
     4141 gtttaatacc ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg gagataaacg
     4201 gtacggcgat gcgtttacca atcaagtctt ccggtttact gatagttttc tttaccacca
     4261 gcgcttcgga gttacccagt tttgacgcca gcaagaagac ttcaatcggc acctgttggc
     4321 tggttgcaac cgctaacggg ctggaaccga ggttgccgat ttgcacatcg cccgaagcca
     4381 gcgcccgcac gatgctggct ccactgtcaa acttacgcca atccacggtt gctccgcttt
     4441 ctttagcaaa ggtgttgtcg gcctgagcca ctttcgccgg ttcggcggag gtttgatacg
     4501 ccacggtgac gttcaccgcc tgcgcctgaa aagcgatgaa tgccagtgcg gcaagaagtg
     4561 tgttacgcga tgaaattgcc atgattgtct gctcccccgt cttgttatgg gagcagtatt
     4621 caggaataaa aacattcatt aaaagaatta gtcgttatcg tacagatgat tttattctta
     4681 gcaaaaaagc ggcaaaggaa agattaaata tgcaattacg cccactctga taactcagta
     4741 aattactgca cttcatttta ttaatgcata aacaagcagg ttctaatgtc tgttatttta
     4801 ttaacagcaa cacatataat gaaaccatta tattcacatt gcatatata
//
LOCUS       DADTFG010000224         4786 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.225, whole genome shotgun sequence.
ACCESSION   DADTFG010000224 DADTFG010000000
VERSION     DADTFG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.225"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            300..1010
                     /locus_tag="J1138_004425"
     CDS             300..1010
                     /locus_tag="J1138_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBA8456119.1"
                     /translation="MLSTTAFLAVAMQCAATVHPSTSLDVARVESGYNPYAIAEIVPR
                     PERKPGDKGFITHMPKSKEDAVSIVTQIEAKGRRYSVGLMQITSTNFKRYGVTATDLF
                     SPCINLSVYEKIITDCYRRGGTLERALSCYYSGNFSTGQQQEPAFSKTSYVQRIGYSP
                     ADTRYAVPGTRDDIATPVATLKATPVDAPTRPRVVWPGAIVRGVPAQLRQKKAATVYY
                     PAQVVRGNRDVTMKEEEK"
     gene            1007..1312
                     /locus_tag="J1138_004426"
     CDS             1007..1312
                     /locus_tag="J1138_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HBA8456120.1"
                     /translation="MKLFRKVNELISRASAYVLAPAMSVFAASPAFAEGFKPANTVMQ
                     KVALGLHGCAAITITVAVVWVGYKTLLNGQSLRDCVYIIIGSILIGGGSEFAALLIS"
     gene            1324..4068
                     /locus_tag="J1138_004427"
     CDS             1324..4068
                     /locus_tag="J1138_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBA8456121.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIIAVWTSVALLFLL
                     PVQFLVMKSLTRNEPMRFSLIAVWLRAKGNPVANRLFGATTFMPVEHDAVDIREFLDT
                     MKLNQCATIRKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDETLRFGANQLN
                     GLIRSFEGMPVTFYVHNSREQFTDSLHRISGNAYADTISELYYTSLGKRPFRRNRLFL
                     TVCYTPFVSLEKAERKRMKDGQKLKELDGALLEMLEIKSSLDTALARYGACPLGTFSE
                     GEMVFSSQLAFYEYLLTHQWRKVRITRTPACDVMGAAALFFSAESGQINHASGTQYFR
                     GLEIKEFSEETATGMMDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLLSSDDDA
                     VSQRLDLDVALDLLTSGKIAYGKHHFSIMVYSPSLENLVADTNEISNALNNIGITPVP
                     AEISLSAAYMAQLPGNFTLRPRKGALSSQNFVELAALHNFYPGKRNNAPWGDAMALMR
                     TPSGDGYYLNLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLEIMQQKYGREDS
                     FSPDARTKRLTTVYLDKDRGAEMNIRALGGRYWRVISGEPTGWNPFSLPATKRNINFI
                     KQLMKILCTRNGATITPRDERRLNDAVNAVMSDEPEYRRFGITRLREQLPEPATKEAQ
                     ENGLDIRLSQWAQGGEFGWVFDNESDTFDISHCDNFGIDGTEFLDDASVCAPISFYLL
                     YRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIK
                     DDIAPAVIEQCGTQILAANPSADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQF
                     RRGDTKRFAARVTLDLSGVGRYTKVMSGDAPNLEIFESIYREGMRPHEWLDTYLAKAL
                     "
     gene            4087..>4786
                     /locus_tag="J1138_004428"
     CDS             4087..>4786
                     /locus_tag="J1138_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HBA8456122.1"
                     /translation="MKTHFYTLIFVCSLASPLAQAGGILVFDATARLENVRQWQKEAK
                     QWMETVQHYKDQMQAYKDQLATATGVRDIAGFVDQARSLKADLEKLRKPGQALNDLLL
                     SGGSSGQFDALYEKYKIFDTCNAERSETYASVCRQQVINKAIQLEQTDEIQKQVSQTL
                     GEINSLSSRVALSRDTKESQDLANSIQLKSVMLNTLTSQWEMSVKAAEKREKMLEAER
                     VKQWSQQQLNAPDIN"
BASE COUNT         1209 a         1256 c         1268 g         1053 t
ORIGIN      
        1 aaagggaatg tgtcccgccc accacttata tacggttcaa aaaaatcgga tcaataccta
       61 agatgaaaaa acacacataa aaaagttgac atgatcttca cttattatag taagccacgc
      121 ctgagtattt tttgacgcca agtaactctg tttctgacta atcgccctta tttgactcca
      181 gggaatagaa aaatttgtca cagagttcag gtcagtcgat accacatttt aacatttcat
      241 tattatccac agcattcgcg gtcaacgaac tctacatgct gtccacacca gaggcaatca
      301 tgctttccac cactgctttc ctggcggtgg ctatgcaatg cgccgccacc gttcaccctt
      361 cgacatccct cgatgtggca agggtggagt ccggttataa cccctatgcc atcgctgaaa
      421 tcgtgcccag gcccgaacga aaacccggcg ataaaggctt tattactcac atgccaaaaa
      481 gtaaggagga tgcagtcagc atcgtcacgc agatagaggc gaaaggtcgc cgctactctg
      541 ttggcctgat gcagataacc agcaccaatt ttaagcgtta cggcgtcacc gctactgacc
      601 ttttcagtcc ctgcatcaat ctttccgtct acgaaaaaat catcactgac tgctaccgac
      661 ggggcggcac cctggaaagg gcgttgagct gttactactc cggcaatttc agtaccgggc
      721 aacagcagga acccgctttt tcaaagacga gttatgtaca gcggattggt tactccccgg
      781 cggatacccg ctatgccgtt cccggcaccc gcgatgatat cgccaccccg gtggccaccc
      841 tgaaagctac gccagtggat gcgccgacgc gcccgcgcgt ggtgtggccg ggggcgatcg
      901 ttcgcggcgt tccggcgcaa ctccgccaga aaaaggcggc caccgtctat taccccgcgc
      961 aggttgtgcg cggaaatcgc gatgtaacaa tgaaagagga agaaaaatga aacttttcag
     1021 aaaggtaaac gaattaatca gccgtgcatc tgcttatgtt ctggctccgg ctatgagcgt
     1081 ttttgcggcc agtccggcct tcgctgaagg cttcaagcca gcgaataccg tgatgcaaaa
     1141 agtggcgctc gggcttcatg gttgtgcggc catcaccatt accgtagctg tagtctgggt
     1201 cggttacaaa acactcctga acgggcaaag cctgcgtgac tgcgtttata tcatcattgg
     1261 cagcattctt atcggcggtg gctcggagtt cgcagcactg cttatcagct aagaggcaaa
     1321 tgtatggcaa agctgctcaa ggccatgaaa cgcccggcag cgctgtgggg ggtaccaatg
     1381 gtgccactgc tggctgtcac gggtgtaacc atcatcattg ctgtctggac ttccgtcgct
     1441 ctcctgttct tgcttcctgt gcaatttctg gtgatgaagt ccctcaccag aaatgaacca
     1501 atgcggttca gtctgattgc cgtatggctg agagcgaagg gaaatccggt agcaaaccgt
     1561 ttatttggcg caacaacatt tatgcctgtt gagcatgatg ctgttgatat cagggagttt
     1621 ctcgacacca tgaaactgaa tcagtgtgcc accatcagga aatacatccc gtactcatcg
     1681 cacattcatc agcatgtggt tcgttccccg aagagcgatc tgtactgcac ctgggaattg
     1741 atgggaacgc cgttcgactg tgagtcagac gaaacactcc ggttcggggc gaaccagtta
     1801 aacgggctaa tccgctcatt cgaaggtatg cccgttactt tttatgtgca taacagccgg
     1861 gaacagttca cggattcact ccatcgaatt tccggtaacg catacgctga caccatcagc
     1921 gaactctatt acacctctct ggggaaacga ccgttccggc gcaaccgtct gttcctgacg
     1981 gtctgctata ccccgtttgt cagccttgaa aaagcagaac gcaaacggat gaaagacggc
     2041 cagaaattaa aggagctgga cggtgctttg ctggagatgc tggaaatcaa aagctcgctt
     2101 gatactgcgc tggcgcgcta tggagcctgc cctctcggta cgtttagcga aggggaaatg
     2161 gtgttttcgt cacagctggc attctatgaa tacctgctga cgcaccagtg gcgaaaggtg
     2221 cgcatcaccc gcaccccggc ctgtgacgtc atgggcgccg cagcgttgtt cttttcggca
     2281 gaatccggac agattaacca cgccagcggt acgcagtatt tccgggggct ggagatcaag
     2341 gagttctccg aagaaaccgc caccggaatg atggactccc ttctttatgc cccctgcgat
     2401 tatgtgatta cgcagtctta tacctgcatg tcacgcgagg aagcgaagaa ggccatcaaa
     2461 cgcacccgcc gtttgttact gagttcggat gatgacgcgg tatcccagcg actggatctg
     2521 gatgtggcgc ttgacctgct gacatcagga aaaatcgctt acgggaagca ccatttttcc
     2581 atcatggtgt attccccgtc gctggaaaat ctggttgcgg atacgaacga aatcagtaac
     2641 gcgctgaaca acatcgggat aacgcctgtt ccggctgaga tatcgctttc agcggcgtat
     2701 atggcacaac tgccaggcaa ctttactctg cggcctcgca aaggggcgct gagcagccag
     2761 aactttgtgg agctggccgc gctgcataat ttctaccccg gcaaacgtaa taacgcgccg
     2821 tggggggatg cgatggcgtt aatgcgcacc ccgtcaggcg acggatatta cctcaatctc
     2881 cacaacacac tggcagataa ggacgaattc aacgagaaga acccggccag tacctgcatc
     2941 ctcggtacga acggttcggg taaaaccatg ttgatgacgt ttctggaaat catgcagcag
     3001 aaatacgggc gcgaagacag cttctccccg gatgccagaa ccaaacggct gacgaccgtt
     3061 tacctcgata aagaccgggg cgcggagatg aatatccggg cgctgggtgg ccgttactgg
     3121 cgggttatca gtggtgagcc tacagggtgg aacccgttct cgctgccggc aaccaaacgc
     3181 aatatcaatt tcatcaagca actgatgaaa atcctttgta cccgtaacgg tgccaccatc
     3241 actccccgcg atgaacgccg tctgaacgac gcggttaatg ccgtcatgag cgatgagccg
     3301 gagtatcgtc gttttggtat tacccggctc agggaacagt taccggaacc ggccacaaag
     3361 gaagcccagg aaaatggtct ggatatccgg ttgtcgcagt gggcgcaggg cggtgagttc
     3421 ggctgggtgt ttgataacga atctgacacg ttcgatatca gccactgcga taacttcggc
     3481 atcgacggaa cggagttcct ggacgatgca agcgtatgtg cgccgatctc gttttacctg
     3541 ctgtaccgca ttaccagcct gcttgacggg cgacgtctgg tcattttcat ggatgaattc
     3601 tggaaatggt tacgcgaccc ggtgtttaag gactttgctt ataacaagct gaaaaccatc
     3661 cgtaagctga acgggatgct ggttgtcggc acgcagtccc cggcggagat catcaaagat
     3721 gatatcgccc cggcagtgat tgagcagtgc gggacacaga ttctggcggc gaaccccagc
     3781 gccgaccgcg cacactatgt tgacggtatg aagtttgagc cggaagtgtt cgacgtggtg
     3841 aaagggcttg atccgcaagc ccgtcagtat gttgtggtga aaaatcagtt caggcgcggc
     3901 gataccaaac gttttgccgc ccgtgtcacg ctcgatctgt cgggtgtcgg cagatacacc
     3961 aaagtcatga gcggcgatgc ccccaacctc gaaatattcg aatcgattta ccgggaagga
     4021 atgcggcctc atgaatggct cgatacctac ctggctaaag cgctctgatt ccaaaggaga
     4081 taagggatga aaactcactt ttacacgctg attttcgtat gttcgcttgc aagcccgctg
     4141 gcacaggcgg gaggtatatt agtatttgat gccacggcca gactggaaaa tgtccgccag
     4201 tggcagaaag aagcgaagca atggatggag accgtgcaac actacaaaga ccagatgcaa
     4261 gcttataagg atcaactggc aacggccacg ggcgttcggg atatcgccgg ctttgttgac
     4321 caggccagga gcctgaaagc cgaccttgaa aagttacgca agcccggaca ggcattgaat
     4381 gatttgcttc tctccggcgg ttcctccggg cagttcgacg cgctgtacga gaagtataaa
     4441 atcttcgata cctgcaatgc cgaacggtcg gagacctatg ccagcgtatg caggcagcag
     4501 gtcatcaata aagccatcca gcttgaacag accgacgaaa tccagaaaca ggtaagccag
     4561 accctcgggg aaatcaacag cctctccagc agggtagcgt tatccagaga tacgaaagaa
     4621 tctcaggacc ttgcaaacag tattcagcta aaatccgtca tgctgaacac gcttaccagc
     4681 cagtgggaaa tgagtgtgaa agcagcagaa aagcgcgaga aaatgcttga ggcagagcgc
     4741 gttaaacagt ggagccaaca acaattaaat gcccccgata taaatt
//
LOCUS       DADTFG010000225         4705 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.226, whole genome shotgun sequence.
ACCESSION   DADTFG010000225 DADTFG010000000
VERSION     DADTFG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.226"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(66..827)
                     /gene="kduD"
                     /locus_tag="J1138_004429"
     CDS             complement(66..827)
                     /gene="kduD"
                     /locus_tag="J1138_004429"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBA8456123.1"
                     /translation="MILNAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSETDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(857..1693)
                     /gene="kduI"
                     /locus_tag="J1138_004430"
     CDS             complement(857..1693)
                     /gene="kduI"
                     /locus_tag="J1138_004430"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBA8456124.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1980..3161)
                     /locus_tag="J1138_004431"
     CDS             complement(1980..3161)
                     /locus_tag="J1138_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBA8456125.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEALARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3416..4645
                     /locus_tag="J1138_004432"
     CDS             3416..4645
                     /locus_tag="J1138_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA8456126.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1225 a         1201 c         1042 g         1237 t
ORIGIN      
        1 tatgatgatc tacagatata aaaaaccctg ccatgcggca gggtcataaa agtaagagga
       61 atgaattaac gcgccagcca accgccatcc acagcaatgg tataaccatt tacataatct
      121 gaagcgctgg aggcaagaaa cactaccggc cccatcaggt cactcggcag tccccaacgg
      181 ccagccggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg cagttgttgg
      241 gtattgttgg tcgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc
      301 cattcgttcg ccatcaatcg cgtcacaccc atcacgccgc tttttgatgc ggtataagaa
      361 ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca
      421 ttgccttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata
      481 ttcaggttca tgacatcgtc ccagtctgtt tcgctgaact cgagagcgtc ttcgcggcga
      541 atcaatccgg cgttattcac caggatatca atatgaccaa actccgctac cgcgcgatcc
      601 agcagtccag gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc
      661 ccctgcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg
      721 tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagcccagt atcacaacca
      781 gtgacgaccg caactttacc ttcgagagaa aatgcattta aaatcataac aataccttat
      841 ttgtttatgc ccacaactag cgcaaatctt taacggccac atggtccata tcatcaaaaa
      901 cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat
      961 ggatcgacca gctcggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
     1021 cttgcggttg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
     1081 aaacttccat ccggcgctcg tgggtgtgac acggcatggt gttccacaag ttacctggag
     1141 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
     1201 tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
     1261 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
     1321 tcgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
     1381 acagggcgtc gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac
     1441 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
     1501 taccaacttc cccgccaacg gaaaccgttt tagttaccgg cataatgccg ccaacaataa
     1561 tacggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
     1621 aaaattcatt gcgcagcccc tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
     1681 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacaataata
     1741 aaacactgtt tcgttttctc tatgagggga tgatatgccc atgagatagg gttttcaatt
     1801 aaaaataaaa caatgtttca ttttcaagtt aaggattaaa aaaagtgtag tgtcgatcac
     1861 gaatatgtca tgagaatgga aaaatcaggg agccctgaaa aggtatgtcg actcaccagc
     1921 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
     1981 tactcgtcac gttcaatggt caatgccaca ccctggcccc cgccgataca aagcgttgcc
     2041 agtcctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
     2101 ccggaagcgc ctatcgggtg accgagtgcg atcgcaccac cattgacatt gacccgacgc
     2161 tcatcccatt caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
     2221 tcgataagat cgacatcagc caactgccag ccaacacgct ccaggcaacg acgggtcgca
     2281 tacaccggcg caattcccat caatgccgga tccacaccaa cgctggcaaa tgcgcggatg
     2341 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattactgca
     2401 gctgccccat cgtttatgga tgatgcatta cccgctgtca cagaaccgag actatcaaat
     2461 gaaggattta aacgggctaa ggcttctgcg ctggtgtcag tgcgtggctg ttcatcggta
     2521 tcaacaacca acgtctgacc gttactttgg gttattaccg ggacgatctc atctttaaat
     2581 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
     2641 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
     2701 tcattgaagg catcccacaa cccatcatgc acaagactat caaccaactg gctattgcca
     2761 agctgtgcgc cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
     2821 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
     2881 gctttaagcc cggaaccaca aacgtcatta atagtgattg cagaaacgct attaggaaga
     2941 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
     3001 ccaagaatca cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttg
     3061 acgaccaggc tgccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg
     3121 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
     3181 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaggtgatt
     3241 tttatcacaa aggaaatacg cctgagtagc agtcagagac ataactggca cgtaaggttt
     3301 gcaaccacta acccaccaat agagaggtag atagggcgtt aatctcccat acttaacctg
     3361 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
     3421 taatatttgg tcaaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
     3481 tgcaggcacg ctcttccttc ctattcagtt aggttcggcg ggggctgtgg tcctgtttat
     3541 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
     3601 cctctcatcg aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg
     3661 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
     3721 gatatatgca gtggcaatta ccaactcact tacagaacag ctggcaaagc atatggttat
     3781 tgatcttcgc atccgtatgt tggtgagtct gggggttgta ttaatcctga atctcatttt
     3841 tctgatggga cgccatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
     3901 ctatttctta tttctttcta tttacctggt cggtagttgg caacctgatc tattaacaac
     3961 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
     4021 tttcgctttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
     4081 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
     4141 cataagtgta ttgttctttg tctttagctg cctgctttct attccacctt cgtatattga
     4201 agcggctaaa gaagaagggg ttaccatttt atcggcgctt tctatgctgc cgaacgcccc
     4261 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
     4321 gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cattacagca
     4381 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
     4441 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag
     4501 cggcccgctc attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc
     4561 ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacc ctgatcgtgg gtatcctgtg
     4621 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gcttcaattt
     4681 tgaaccatcc cagttttcat aatgt
//
LOCUS       DADTFG010000226         4684 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.227, whole genome shotgun sequence.
ACCESSION   DADTFG010000226 DADTFG010000000
VERSION     DADTFG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.227"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            99..929
                     /locus_tag="J1138_004433"
     CDS             99..929
                     /locus_tag="J1138_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA8456127.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            961..1896
                     /locus_tag="J1138_004434"
     CDS             961..1896
                     /locus_tag="J1138_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA8456128.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1989..2231
                     /locus_tag="J1138_004435"
     CDS             1989..2231
                     /locus_tag="J1138_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBA8456129.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2221..3672
                     /gene="panF"
                     /locus_tag="J1138_004436"
     CDS             2221..3672
                     /gene="panF"
                     /locus_tag="J1138_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBA8456130.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLAKLNIQYQGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3684..4565
                     /gene="prmA"
                     /locus_tag="J1138_004437"
     CDS             3684..4565
                     /gene="prmA"
                     /locus_tag="J1138_004437"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBA8456131.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFTHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
BASE COUNT         1059 a         1173 c         1238 g         1214 t
ORIGIN      
        1 tttttgaccc atgattttct tttcgaattg tcgatatgat tgaaggataa ctttcaaaga
       61 aaaatacaag gggctatagt gttacacatt aaaggccaat gaagagtgtt aacgtgccga
      121 gaaaatcgtc taacctactg aaacgcagat taaaaattgc agaaattgtt gccgcccagg
      181 gtgaaatcaa agttgatgac ttatctgcgc aacttggtgt ctctggcgtg acgatccgcg
      241 gcgatctcag ttatctggag caacagggat atttaaagcg atcatttggc ggtgctattg
      301 cgacaccact ctcctctgaa ccagaagcga tacaaccgat cgcgccattg tctctggcac
      361 aacaaatgga aatagcacga cattgcgcac gattaattcg cgatcgtgac acgctatttc
      421 ttggacatgg gacaatctgc cgtaagatca ttccattgct cagcgaagtg aaaaaactgc
      481 gtctgatcac caacgatccc gaacatgcgc tgctggcaaa tcagtttatt gatggcgaaa
      541 taatactggc tggcagtgaa atgttgcgcc ccgacaccgc actggccgga aaacagcttg
      601 aacaggcact tcagcatttc accattacac atagcatcct ggaaatcagc catctggatg
      661 ctgatggtac tttaaatatc aacgttccca gactcgccgc tgcctggcag ctctgtttcg
      721 atcatgccca gaataagacg gttatcgttg ccgccgatcc tgcgccatca acggctgcta
      781 tcgggcattt gcatcaggca gaaaatgtga tcgtcagccg cagcattaac gaacaatatc
      841 agcaacaatt gcagtcggct gatttcgtta tcctttatac cagtaatgag tgctttacct
      901 ggtccaatcg cgaaaggctt caggaatgag aggggttttg tgacaacaac tatctgcgct
      961 atgggcgaat tgctggccga gtttttgtcc cgcaacccac atcaaaaatt cactcagcct
     1021 ggggagttta tcgggccatt tcccagcggt gcgccagcaa tttttgctgc tcaggtggca
     1081 aaactgtccc atcgggccat cttctttgga tgtgttggta atgatgattt tgcccgactc
     1141 attatagagc gtctccgtca tgaaggtgtc attaccgatg ggatccatgt tatgaacaat
     1201 gccgtcacag gtacggcgtt cgtgagttat caaaatcccc agcagcggga tttcgtcttt
     1261 aatatcccta acagcgcctg cggtttgttt actgccgagc acattgataa ggatctgctt
     1321 aaacagtgta accatctgca tattgtgggc tcatcgttgt tctcatttcg catgatcgat
     1381 gtcatgcgta aagcaataac gacgatcaaa tcggctggcg gcaccgtttc tttcgatccc
     1441 aatattcgca aagagatgct gagcattcct gaaatggcgc aggctctcga ttatttgatt
     1501 gaatatacgg atatttttat ccccagcgaa agcgaactcc ctttcttcgc gcgtcacaaa
     1561 aatctgtcag aggaacagat tgttagcgat cttctccacg gcggcgtaaa acatgtggcg
     1621 ataaaacgcg cccagcgtgg ggccagctat tacaagctta aaaacggtac attacacgcc
     1681 cagcatgttg caggtcacga tatcgaaatt atcgatccaa cgggtgcagg cgactgcttt
     1741 ggcgcaacgt ttatcactct tttcttatcc ggtttcccgg cacacaaggc gctgcaatat
     1801 gcaaatgcca gcggcgcgct cgccgtaatg cggcaaggtc cgatggaagg gatatcctca
     1861 ctggcagaca ttgaagactt tttgcagcag cactgaacgg caattttctg tcagatcatt
     1921 ttccctgcct gaagacttgc cgtagaattg cccgtttctc gtatcccccc atgaggagga
     1981 ccccttccgt ggacactcgt tttgttcagg cccataaaga ggcgcgctgg gcgctggggc
     2041 tgaccctttt gtatctggcg gtatggttag tagccgctta cttacctggc gttgcccccg
     2101 gttttaccgg ctttccgcgc tggtttgaga tggcctgcat cctgacgccg ctgctgttta
     2161 ttggactgtg ctgggcgatg gtgaaattta tctatcgcga tatcccactg gaggatgacg
     2221 atgcagcttg aagtaattct accgctggtc gcctatctgg tggtggtgtt cggtatctct
     2281 gtttatgcga tgcgtaaacg gagcactggc accttcctta atgagtattt cctcggcagc
     2341 cgctctatgg gcggtattgt gctggcgatg acgctcaccg cgacctatat cagtgccagt
     2401 tcgtttatcg gcgggcctgg agctgcttat aaatacggcc tgggctgggt attgctagcg
     2461 atgattcagc ttcctgcagt ctggctttcg ctcggtattc tcggcaagaa gttcgcaatt
     2521 cttgcgcgcc gctacaatgc ggtgacgctg aacgatatgc tgtttgcccg ctaccagagt
     2581 cgtcttctgg tatggctggc gagtttgagt ttgctggttg cgttcgttgg tgcgatgacc
     2641 gtgcagttta tcggcggtgc gcgcctgctg gaaaccgcgg cgggtattcc ttatgaaacc
     2701 gggctgctga ttttcggtat cagcattgcg ttgtataccg cctttggcgg ctttcgcgcc
     2761 agcgtgctga acgacaccat gcaagggctg gtgatgctga ttggcaccgt tgtgctgctt
     2821 attggcgtgg tacatgccgc aggtggttta agtaatgcgg tagacacgct gcaaaccatc
     2881 gatccgcaac tggttacgcc acaaggcgct gacgatattc tgtcgcctgc ctttatgacg
     2941 tcgttctggg tactggtgtg ctttggcgtt ataggcctgc cgcatactgc ggtgcgctgt
     3001 atctcttata aagacagcaa agccgtacat cgggggatca ttatcggtac gattgtagtc
     3061 gcaatcctga tgttcggtat gcatctggcc ggagcgttag gtcgggcggt gatccccgat
     3121 ctcaccgtac cggatctggt gatcccgacg ttaatggtaa aagtgctgcc accgtttgcc
     3181 gccgggatct tcctggctgc accgatggct gcgatcatgt cgacaattaa cgcccaactg
     3241 ctgcaaagtt ccgctacgat cattaaagat ctctatctga atatccgtcc ggatcaaatg
     3301 caaaacgaga cgcgtctgaa gcggatgtcg gcggtgatca cgttggttct cggtgcgttg
     3361 ctgctgcttg ccgcctggaa gccgccagaa atgatcatct ggctgaattt gctggccttc
     3421 ggtgggctgg aagccgtttt cctgtggccg ctggtgctgg gtctttactg ggaacgtgcc
     3481 aacgccaaag gcgcgctaag tgcgatgatc gttggcggcg tgctgtatgc cgtactcgcg
     3541 aagctgaata ttcagtacca gggcttccac cctatcgtgc cctcgttact actaagtttg
     3601 ctggctttcc tggtcggaaa ccgtttcggt acatccgtcc cgcaagctac cgttttgact
     3661 actgataaat aaagagtttt gccatgcctt ggatccaact gaaactgaac actaccggcg
     3721 cgaacgcgga agatcttagc gatgcgctga tggaagcggg tgccgtttct atcacttttc
     3781 aggataccca cgatacgcca gtatttgagc cgctgccggg cgaaacgcgc ctgtggggcg
     3841 acaccgatgt gattggtctg ttcgacgctg aaaccgatat gaacgacgtg gtggcgattc
     3901 tggaaaacca tccgctgctc ggcgcgggct tcacgcataa aatcgaacaa ctggaagata
     3961 aagactggga gcgcgaatgg atggataatt tccacccgat gcgctttggt gaacgactgt
     4021 ggatctgccc aagctggcgt gatgtgccgg acgaaaacgc cgtcaacgtg atgttagacc
     4081 cagggctggc gtttggtacg ggtacccatc caactacctc tctgtgcctg caatggctcg
     4141 acagcctcga tttaaccggt aaaacagtca tcgactttgg ctgcggttcc ggcattctgg
     4201 ctatcgcggc gctgaaactg ggtgcagcaa aagccattgg tattgatatt gatccgcagg
     4261 cgattcaggc cagccgcgac aacgctgaac gtaatggcgt ttctgatcgt ctggaactct
     4321 acttaccgaa agatcagcca gaagaaatga aagccgacgt ggtggtcgct aacatccttg
     4381 caggtccatt acgtgaactg gcaccgttaa tcagcgtcct gccagtttca ggcggtttgc
     4441 tgggcctttc cggtattctg gcaagccagg cagagagcgt ttgcgaagct tatgccgaca
     4501 gcttcgctct ggacccggtc gtggaaaaag aagagtggtg ccgcattacc ggtcgtaaga
     4561 attaaccttc gcatcgccgt atgacgacgc agagcgaagt gcgagcgatg tcacaaaagg
     4621 cacgttaatt tgccgattat ttacgcaaat ttgcgtgcca aaattttcat tcataaagaa
     4681 aaat
//
LOCUS       DADTFG010000227         4668 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.228, whole genome shotgun sequence.
ACCESSION   DADTFG010000227 DADTFG010000000
VERSION     DADTFG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.228"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..665
                     /gene="hprR"
                     /locus_tag="J1138_004438"
     CDS             <1..665
                     /gene="hprR"
                     /locus_tag="J1138_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBA8456132.1"
                     /translation="LLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDDYAL
                     IILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFS
                     ELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLASRA
                     GEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAV
                     KK"
     gene            665..2023
                     /locus_tag="J1138_004439"
     CDS             665..2023
                     /locus_tag="J1138_004439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HBA8456133.1"
                     /translation="MKRLSITVRLTLLFIFLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRIKIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVVIILCSLLSPWLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDTLALNKEVENLLDYLEYLSDEKEIRF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2131..2982)
                     /gene="hchA"
                     /locus_tag="J1138_004440"
     CDS             complement(2131..2982)
                     /gene="hchA"
                     /locus_tag="J1138_004440"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBA8456134.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRRSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(3578..4636)
                     /locus_tag="J1138_004441"
     CDS             complement(3578..4636)
                     /locus_tag="J1138_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456135.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVELYGKMVGERIL
                     TDRENGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYA
                     GLSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTD
                     FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQS
                     GDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDF
                     GLRPSIGYFYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVL
                     AHGLNTDDQLAVGIVYQF"
BASE COUNT         1311 a          939 c         1084 g         1334 t
ORIGIN      
        1 ttctacttat tgaagataat caaaggaccc aggaatgggt aacgcagggg ctttccgaag
       61 cgggttatgt cattgatgcc gtttctgatg gcagagatgg gctttatctt gcgctgaagg
      121 atgattatgc attgatcatt ctggatatta tgcttccggg tatggatggc tggcagatct
      181 tacaaacgtt aagaacagca aagcaaaccc ctgttatttg ccttactgca agggattctg
      241 tcgatgacag agtcagaggg ctggacagtg gggcaaatga ttatctggta aaaccttttt
      301 cattttctga gttgctggca agggttcggg cacaattaag gcaacatcac gctctgaatt
      361 caacattaga aatcagtggc ttaaaaatgg actctgtcag tcaaagtgtg agcagggaca
      421 atatcagtat tacactgacg cgcaaggagt ttcagttact ttggctactg gcctccagag
      481 ctggcgagat tatacccaga acggttattg cgagtgaaat ttggggaatc aactttgata
      541 gtgataccaa tacggtggat gtcgccattc gcaggctccg cgcaaaagtt gatgatcctt
      601 ttcctgaaaa gctaatcgcc acaatccggg ggatgggcta ttcattcgta gcggtaaaaa
      661 aataatgaaa agattatcta taaccgtccg tttaaccttg ctttttatat ttctgctgtc
      721 tgttgctggc gctggaattg tatggactct ctataatggc ctggcaagtg agttgaaatg
      781 gcgcgatgat acaacactca ttaaccggac agcgcagatc aagcagttgt taattgatgg
      841 ggtaaatcca gatacattac ctgtctattt taaccggatg atggatgtta gtcaggatat
      901 cttgatcatt catggtgatg gcatcaataa aattgttaac cggacaaatg tcagtgatga
      961 catgttaaat aacatacctg ctagtgagac aatcagcgca gcaggcattt acagaagcat
     1021 tattaatgat acagagatag atgctttacg aattaaaatt gatgaagttt cgccatcatt
     1081 aacggttact gtggctaaat tggcttcagc cagacataat atgcttgaac agtataaaat
     1141 taatagcatt ataatttgca ttgtcgtcat tatattgtgc tcattattaa gtccgtggtt
     1201 aatcagaacg gggttgcgag agatcaaaaa gttgagtggc gtaacggaag cgctgaatta
     1261 taacgatagc cgagtgccgg ttgaggttaa cgcattaccg agagaactaa aacctcttgg
     1321 gcaggcgttg aataaaatgc atcatgcttt agtcaaagat tttgagcgcc ttagtcaatt
     1381 tgctgacgat ctcgctcatg aacttagaac gccaattaat gcattactgg gtcagaatca
     1441 ggttacgctc agtcaaacca gaagtatcgc tgaatatcaa aaaacaattg ccggaaacat
     1501 tgaagagctg gaaaatattt cgcggttaac agagaatata ctgtttcttg ccagggcaga
     1561 caaaaacaat gttttggtga aactggatac gcttgctctc aataaggaag ttgaaaatct
     1621 tttggactat cttgaatatc tttcagacga gaaagagatt cgttttaagg tcgagtgcaa
     1681 tcaacaaatc tttgcggata aaattttact gcaacgaatg ttatcgaatc ttattgttaa
     1741 cgccattcga tattcaccag aaaaatcgcg tattcatata accagttttc ttgatgccaa
     1801 cggttctctt aatattgata tcgccagtcc tggaacgaaa attaatgagc ctgaaaaact
     1861 cttccgtaga ttttggcggg gagataattc gcgtcattcc gtaggtcagg gacttggcct
     1921 ttctttagtc aaagcgattg ccgaattaca tggaggaagt gctacgtatc actatctcag
     1981 taagcataac gtgttccgaa ttacgttacc gcaaagaaat taacatcggg cttcagtacg
     2041 ccataagtta accgtgaaaa aacgcagcca cgtcgtgagt attaacgtgg ctgcgattga
     2101 ttatgcgctt acatcacaat gtaacaggga ttaactcgcg taagctgcca gcatttcctg
     2161 cgccgccagt ttacccaacg cattcgctgc gaaaggactg tcgccggtga gaagtttacg
     2221 gtccttatgt actcgcccgg tgatgtcgtc attaatgatg ttcatgccca ttttcttcag
     2281 ctcttcgccg aagtaccagg tgagatgacc cggcatatag ccaatatctg gcgtttgttt
     2341 gtctgcggcg tctgggaatg cgcaaataga ataaccattc agtgggttat cgctgcggcg
     2401 aagtgccaga aaagccgccg gaccgtggca aagggagata acaaaacggt catttttgat
     2461 tgcccactgt aaagcggcag ccacgtcctg gctttctggt aaaccaataa gtgcgccatg
     2521 cccaccagga acaaagattg ctgcatattc gctatcagcg ttgaggctgg cgacaacatc
     2581 cgcgagtttc ttcggattac ggaataacga tttatgctgt tcaaagaatg gcattacttt
     2641 ttcatctttg tgcggcatag cccagtattc aaacttggtc atcagaccgg aaatggtcgc
     2701 cacttcgaat tcgaaacctg cagcatggag atgatacaac ggcagcaacg tttcaatcgg
     2761 atggttaccg gtcgagaaca gttttccgtt atcggtcggc aaataacgtt cgtccgcggc
     2821 gatcaccaga attttatgtt taccgcgata cggttttgga tagtccacgc catcaagatc
     2881 agagacggga ctcgtatatt ggctaagcga atattctgaa gggaagaacg cattatcttc
     2941 agcaatatcg acctgcgggt ttttacttgt ttgaacagtc atagtgtatt ccttattgtt
     3001 gcttagttag ggtagtcact atatttgcga ctgagctggt cgctattttt aaccaggctg
     3061 aaaaattgag tcaatatatc gaaagcggta gttggcgggc ataagtatgt aatgatgatg
     3121 ctgcactggt gttgaatatt attcacgcca aactttcctt attgagtaac gtcgtcgcaa
     3181 gtggcctcaa tgattttaga ccattatcag aggtttgggg gcgatgggga gagatttagt
     3241 gctgtggaat gttgtggtac tgatgaatat ttatatcccg gattggtagg gaaaaagctt
     3301 ttttcatgca ctatttttgc aaatatttct atatgggaag cggttttgtt acagattaaa
     3361 tttataaaga aatgcatttg gggtgttggc tggaagagtt gctggtagag gatattgtgc
     3421 caggaggggc agggtaagtg gctgtatcga gatgctttgg caattgcgtg tgccgcagat
     3481 ggagatgaaa tgccaataaa aaaagcctga gttcgatgag ggacgaactc aggctggtgt
     3541 tatgttttac agtaacacgc ctcggcgcgt tggctgatta gaactggtag acgataccaa
     3601 ctgccagttg gtcgtcggtg ttcagtccat gagcgagaac ataatcactc ttatcaagca
     3661 ggttgatctg ataagcggta tatacgttca tattcttatt gaagtaatac caggtaccga
     3721 tttcaatata gttcagacga tcagaatctt tgtaacctgc tacgtccagt gctttagaat
     3781 agaagtaacc gatggatgga cgcagaccaa agtcgaactg atattgcaga acagcttcga
     3841 agttttgtgt tttgttcaca acatcaccgt cgtcggaatt catgttgtga gattcaccgt
     3901 acatcacagc agcatagaca ttgttagcgt catattttgt tgacaacgcc caaacttcgg
     3961 ctctttcgcc tttaccatca ccagactgcc aatcagtacg gtcagagttt gcataagcac
     4021 cagcaaatga gaaaccgtca atagtgtaag ttgcagataa accatggcca tcgccatttg
     4081 ctttgtagtt gcctgtacct tcatttttac cctgatattg cagagcaaaa tttagacctt
     4141 caaccatgcc aaagaaatct gtgttacgat aggttgcaac accgttggta cggttggtca
     4201 tgaacaggtc agtaccagcc catgagtcac cgccccactc aacaaacata tcagtaaatg
     4261 cttctgcatc atacgcgaca ccgacattac gaccgtagtc gaatgaacca aaatctttgt
     4321 aactcaaacc agcatatgcc aaacgagttt ggtcagggtt gtgtctgttt gatgcttcca
     4381 ggtctagttc aaactgaccg taaccagtca gttcagggtt gatctgagtt tcacctttca
     4441 cgcctacacg ggcataagag gtatcttgag aattatcgcc tttttcacca ttttcacggt
     4501 cggtcagaat gcgctcacca accatcttgc cgtatagctc aactttattg ccatttttat
     4561 tataaatttc agccgcattt gctgcgccag caactaataa cgccgggacc agcattgcca
     4621 gaacttttct tttcattatg tattcccttg tgattataat cttcatga
//
LOCUS       DADTFG010000228         4614 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.229, whole genome shotgun sequence.
ACCESSION   DADTFG010000228 DADTFG010000000
VERSION     DADTFG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.229"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(259..936)
                     /locus_tag="J1138_004442"
     CDS             complement(259..936)
                     /locus_tag="J1138_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016538809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA8456136.1"
                     /translation="MLLLETLVTRNEGFRLAELSEQFSLNKTTLHGLLATLSSLGYVS
                     RRGQHYIAGLRLRDVARPLTETDQQLREHFTPALRRLAALSSETCWLAVPCGTREYLY
                     IDAIEGGSSLRVASPRGTREGLTTSAIGRVFLAFDDDLTRTLRKEGFITDKLNNELAC
                     IAKCGYALDLEEAEEGLNCLALPLRQQGYVVAALGMAGPSHRLHPDYLKRLAVRVMRE
                     MFDIIKL"
     gene            1230..1865
                     /locus_tag="J1138_004443"
     CDS             1230..1865
                     /locus_tag="J1138_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2913 family protein"
                     /protein_id="HBA8456137.1"
                     /translation="MLDKTKSVSTDNTTAALSHLAFCALAALGLARQDGIVGTPYAEN
                     LFLIRWLATAQKQKRFPRSVAVDLTWLLERGRKHGPAGKLRQHLEYLWRSCSGNLAAQ
                     SDLFRLTYATETLKDRDWDNYVMDAREWRSETTLTPSQHNGFYVEKTALNAAFSQDGH
                     HLQPVSFRIVGDVDTFIQVMAEYGLLARLADSKPTYQTVTLEPGEHSNAPT"
     gene            2134..2352
                     /locus_tag="J1138_004444"
     CDS             2134..2352
                     /locus_tag="J1138_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456138.1"
                     /translation="MPKGIRLPTLVGETPQRLHISYVNNWGKFIGRNVPWLGYALSAW
                     TIYAIGRDVKDRFNLIARPADRIQWTYF"
     gene            2352..2678
                     /locus_tag="J1138_004445"
     CDS             2352..2678
                     /locus_tag="J1138_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HBA8456139.1"
                     /translation="MMKTPAQIEQEIIDYYYENFSPRKYVFWGPKEQVTLDTRLREDL
                     KIGWDDGDDAINTYLSIWHVDPAGMEFTHYFAPEFFGSKVPEQPLKPLTIRMLVESAK
                     AGRWLY"
     gene            complement(2675..2920)
                     /locus_tag="J1138_004446"
     CDS             complement(2675..2920)
                     /locus_tag="J1138_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8456140.1"
                     /translation="MTGFELKLWRTGLGWTQERASKEFGISMRTYIRYEHADPPRAIE
                     LAIQALSLAALLPEIDALPAETLRQRVRMLAVDIQSG"
     gene            3125..3379
                     /locus_tag="J1138_004447"
                     /pseudo
     CDS             3125..3379
                     /locus_tag="J1138_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
     gene            3573..4136
                     /locus_tag="J1138_004448"
     CDS             3573..4136
                     /locus_tag="J1138_004448"
                     /inference="COORDINATES: protein motif:HMM:NF025054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA8456141.1"
                     /translation="MNRLVTIRRASAHDAETLGVVGPAAYAAAYHYLWSDCVALAHQL
                     DTFSKSAFLVLLQRDNSRVWVAEISGKIVGFLTMLINSENPVTHDISGAEISRIYLLP
                     GSQKMGIGMQLLNAAQEHASELELTHMWLDVMESATAARNAYYKWGFSDIGSKDFPRQ
                     VKADLSKMIVLSIRLNAKGNRTLSCMR"
     gene            4139..4609
                     /locus_tag="J1138_004449"
     CDS             4139..4609
                     /locus_tag="J1138_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA8456142.1"
                     /translation="MELRRFVDGDEIALLNVFLSSVRHIASHYYTHEQIEAWASPDMD
                     PEQWGNYMRELRPFIVEVGGEIAGYADVQLNGYIDHFFVSGTYPRQGVGTLLMNTIHE
                     KARQLGVCELTSNVSKAAEAFFLRHGFHVVERGFPVRRGVVLQNALMRKKLLKP"
BASE COUNT         1215 a         1163 c         1162 g         1074 t
ORIGIN      
        1 gtgcgtgctt ttttaatcga aacatcaatc gcgccggcaa cagcaccgtc cattcgcagg
       61 aaacttctca gcaggcctgc attgattgtc cacaatactg atacaaacag cctggtgttg
      121 ctcctgagcc cgtgaggtga ttgtacgaat ggcatcacgt gaaattttct caaacatata
      181 aaaacactcc ggtcgatgaa agagtgtttt ttatagcgga cctggcttta ttgcccaagt
      241 aatcccagct atcagccttt aaagtttgat aatgtcgaac atttcccgca tcactctgac
      301 agccagtcgt ttgagatagt caggatgcag gcgatgtgaa ggacccgcca ttcccagcgc
      361 ggcgacaacg taaccctgct gacggagagg gagcgccagg cagttcagtc cttcctcagc
      421 ctcctctaaa tcaagtgcat atccacattt agcgatacag gccagctcgt tattgagttt
      481 gtcagtaata aacccttcct ttctgagggt tctcgtcagg tcatcatcaa aagccagaaa
      541 gacacgcccg attgctgaag tcgtcagtcc ttcccgtgtt cccctgggac tggcgacccg
      601 cagtgaactt ccaccttcaa tcgcgtcaat atagaggtac tcacgtgtac cgcagggcac
      661 cgccagccaa caggtttcgc tactgagcgc agccagcctg cgtagtgcgg gagtaaagtg
      721 ttcgcgaagt tgctggtctg tctccgtgag tggccgggca acatcccgta agcgtagacc
      781 tgcaatataa tgctgccccc ggcgtgaaac gtatccgagc gaagagagcg tggcaagtaa
      841 cccatgtaac gtggttttat tcagtgaaaa ctgttctgac agttcggcca gacggaatcc
      901 ttcgttccgg gtgaccagag tttcaagaag caacatggcc cgctcgacgg actgtatgta
      961 tttacgctga gtcacggcct gatatcctca gaaaaatcat gatgaaaaga gagtaacagg
     1021 tcggaagcct ttcaggcata ggctggttgg ataagagatg gtcggttacg gtgttcggtt
     1081 ttgtagtgac tccggagtag aaccccacat tggtaattat ttggtgtgca atgccttgct
     1141 ggtcactgcc cggattgcca gttacactga atggtgatgg atcgcagagt ccctttaacc
     1201 attcgtcaaa acgggcaggg cagacaacgt tgttagacaa aaccaaatcc gtttctacgg
     1261 ataacaccac cgccgcactc agccacctgg cgttctgcgc cctggcagca ttggggctgg
     1321 cccgccagga cggcatcgtt ggtacaccct acgccgaaaa cctgttcctg atccgctggc
     1381 tggccaccgc ccaaaaacag aaacgatttc ccagaagcgt cgccgtggat ctcacctggc
     1441 tgctcgaacg cggccgcaaa cacggcccgg ccggcaaact gcgccagcat ctggaatacc
     1501 tgtggcgctc ctgcagcggc aatcttgccg cgcagtccga tttgttccgg ctcacttacg
     1561 cgactgaaac cctgaaagat cgggactggg acaactatgt gatggatgcc cgcgagtggc
     1621 ggtcggaaac caccctcaca ccgtcccagc acaacggttt ttacgtcgaa aaaacggcgc
     1681 tcaatgccgc cttctctcag gatggccatc acctgcagcc ggtctctttt cgcattgtcg
     1741 gcgatgtcga tacgttcatt caggtcatgg cagagtatgg actgctggcc cgccttgcag
     1801 acagcaagcc gacatatcaa acggtaacac tggagccagg cgagcacagc aacgcgccaa
     1861 cctgacatcc cctacggtaa aggataacct atggatacgg ttgaggaatt aaacggtacg
     1921 tatttctaca aaggatatac ccagttgacg ccccagcagc tgtggtggct tatcaccgtc
     1981 gatgcgctcc gtgaacatct ggggatcagc attgccgatg ccgtcctgct cgcatcaggg
     2041 caaccctggc ttcccacgcg ccaaaagccg gggaccgcta caccaggcac gtcaatcgcc
     2101 tcgcgactgt cacggaaata cctggacaag cctttgccga aaggtatccg tttaccgaca
     2161 ctggtgggtg aaaccccaca gcgcctgcac atcagctacg tgaataactg gggaaaattt
     2221 atcggccgca atgttccctg gctggggtat gccctgtcgg cctggaccat ttacgcgatc
     2281 ggccgcgatg tgaaagaccg tttcaacctc atcgcccgtc cggcagacag aattcagtgg
     2341 acctattttt gatgatgaag acgcccgcac aaatagagca agagatcatt gattattact
     2401 acgaaaattt cagcccgcgg aagtatgtct tctggggccc gaaagaacag gtaaccctgg
     2461 acacacgctt gcgggaagat ctcaagattg gttgggatga cggtgacgat gcgatcaaca
     2521 cctacctgtc catatggcat gtcgatccgg ctggcatgga attcacccac tattttgccc
     2581 cagagttttt cggttcaaag gtgcccgaac agcccctaaa accgctgacc atcagaatgc
     2641 tggtcgagtc ggccaaagcc ggacgctggt tgtattagcc ggattggatg tcaaccgcca
     2701 gcatcctgac gcgttgtcgt aacgtttctg caggaagagc atcaatctcc ggtaacagcg
     2761 ccgccagaga cagggcctgg atggccaact caattgccct cggcggatca gcatgctcat
     2821 agcggatgta ggtacgcatc gaaatcccaa actcctttga cgcccgttcc tgtgtccatc
     2881 ccagtcccgt tcgccatagt ttcagctcaa atccagtcat cacagcccct aaaacatgtc
     2941 attttgtcat attattgcaa tgcaatttgg caaacaatga cgccagtagc aagtgacgat
     3001 cacgcctgga aacacaacat ttagtacctc attcagctgc tttagacaca atatcggggt
     3061 ctgacgctca gtggaacgaa aactcacgtt aagaggtttg gctgtgagag aggacaaaga
     3121 aactctcgac ctgatggccg cgatgtctca taaggactgg ctgagccgtc gccagcgcca
     3181 aaaacaggga attgaacggg cccatacgca gggtaaatac cggggtaagc aggccgatct
     3241 ggagcgtcac cagaaagtcc tctactatcg gcagactaag aagctgagca ttcaggaaac
     3301 agctgaagct acgggctata gtcgttcaca agtctgccgt atacagtccc tttaccgaca
     3361 ggaagataaa ggattatgaa taaatacaaa ttcaaaccct ggctgagtca ctgggaactc
     3421 attccagatg gcgctccatt tgtagattta catcatggta acaggctatc caatgccgaa
     3481 tgaatcagaa cgattattct ggaaaactct aaaaccgtgg ccaatttttg ttgaccactg
     3541 catacaaatt cccccagatg gggagggata gattgaacag attagtaaca attaggagag
     3601 catctgcaca tgatgctgag acattgggtg tggttggtcc tgccgcttat gctgcggcat
     3661 accattacct gtggagtgac tgcgttgctc tagctcatca actcgatact tttagtaaat
     3721 cagcattttt ggtactgtta caacgagata actcaagggt atgggttgcc gaaatatcgg
     3781 gtaaaattgt tggttttcta acaatgttaa taaattcgga aaatccagtt acacatgata
     3841 ttagcggtgc agaaatttcg aggatttacc tacttccagg tagccaaaag atggggattg
     3901 gcatgcaact gctgaatgca gcacaagaac atgcgagtga acttgagctt acgcatatgt
     3961 ggcttgatgt aatggagtca gcaacagcag caaggaatgc gtattataaa tggggttttt
     4021 cagatatagg gagtaaagat tttcctcgtc aagtcaaagc agatttatcg aaaatgattg
     4081 ttctgagtat aaggctcaat gctaaaggca acagaacatt gtcgtgtatg aggtaattat
     4141 ggaactacga agattcgtag atggtgacga aattgctttg ctcaatgtat ttttatcctc
     4201 tgtacgtcac atcgcgtcgc actactatac acatgagcaa atagaggctt gggcttctcc
     4261 agatatggac ccggaacaat ggggcaacta catgagggaa ttgcggccat ttatcgttga
     4321 ggtgggtggc gaaatagcgg gatacgcaga cgtccagctg aatggataca tcgatcactt
     4381 ctttgtatcg ggaacctatc ccagacaggg agtagggaca ctattgatga ataccattca
     4441 tgaaaaagcc agacaactgg gcgtttgtga acttacgtca aatgtaagta aggcagcaga
     4501 agcattcttt ctacgacacg gttttcacgt agtggagcga ggttttcctg ttcgccgtgg
     4561 cgtagtattg caaaatgcat tgatgcgaaa aaaactcttg aagccataaa aagc
//
LOCUS       DADTFG010000229         4573 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.230, whole genome shotgun sequence.
ACCESSION   DADTFG010000229 DADTFG010000000
VERSION     DADTFG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.230"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..646)
                     /locus_tag="J1138_004450"
     CDS             complement(<1..646)
                     /locus_tag="J1138_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBA8456143.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQIQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPA"
     gene            complement(662..3142)
                     /locus_tag="J1138_004451"
     CDS             complement(662..3142)
                     /locus_tag="J1138_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBA8456144.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVSNI
                     RLEDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDSFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYM
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQVGHQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
     gene            complement(3158..3832)
                     /locus_tag="J1138_004452"
     CDS             complement(3158..3832)
                     /locus_tag="J1138_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA8456145.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKVTFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(3913..4455)
                     /locus_tag="J1138_004453"
     CDS             complement(3913..4455)
                     /locus_tag="J1138_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8456146.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDGAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLIMRVAQD"
BASE COUNT         1294 a         1100 c          843 g         1336 t
ORIGIN      
        1 tggctggtaa ttgtgaaacg ggaatactct ctaacgtaat taataaatcg tccggtttgc
       61 aggttgtggt tatcgggttg tatgacaatg tcagattaaa ggaatagaca cctgcatttt
      121 gtgccaatgc atttctgtag ctatttacac atgcatccca tgacttacca cctaataaac
      181 acagtagaaa ttgtactgcg gcactcccgc gtgtcgatga tgaagcactg gataatgtcg
      241 caactccatt aatcatcact gaaccaccgc cccccgcaga aacattcact tttgcattac
      301 tggccgcagg tgtcaccgtt accgaataac cactggtgaa actggtctga ttatttgaca
      361 acgtaatgtt gtttttattg gtccattcaa aatgtaaatt taatttaatg gtatcgttat
      421 aaagtccaac aataatatcc gacgggctca taggggtgat agtatttgta ctcacgcatg
      481 acgaatcatt aaaattatgc tcatatgtcg ccatttgtat ttgagtaagt gcaaaatccc
      541 cctgaacaac caaattgtgc tgaacagata actcactata acatgccgca tagctcacag
      601 gcataaccat catcattact ataaatagca ttatgcgaat ttccatagaa ccacctgact
      661 attactggca aatataattt ttactttcat caatctcttt accgaaggtg attgtgcatg
      721 aaagcccttg ttgcttatca attgccacat taaccgatgg cggtacttca ttggtgcgaa
      781 taaataactg gcttccctgg ccgacaacac caatattatg accatggata tcattgactt
      841 cataaccaaa cgttaatggt tgcccatccg ctcttaacgc ttttataaac catggcttgc
      901 gctgatcggt atcaaaatta accagtacaa ccgcgccgcg ataaggggcg gcaattttcc
      961 ggttgccacg taattctgct tcgctatcgc tttgtgacac atccaacatc aggtaatttt
     1021 cccgataagg tgtcattccg tcgtatacca ccactccatt acggtttgtt gtgcgatatt
     1081 tttgcccatt gacataagca tctttaattc ctggcgcatt catcacagca aacgtttcag
     1141 aaagacggtt tgccagatta acgccacccg accaagcgac aatgccccct gaaacactgg
     1201 ctccagcctg tcgataagca ctcgactgac tataactgcc attcactgtc gcaaccggcg
     1261 cgttccaggt taaattcgcc ccagctgtcg tttcatttcc ctgatactga taactcaggt
     1321 tgaccccata gttaaactga tcccggcttc caacggttcc tgataatccc gtattatttg
     1381 aggcaacccc ctgatcatca aacgtcgttg agttagacat atatatttgc cgacgaggcg
     1441 tcgtaacgtc atcaccccaa tcaaagggaa tcgatataaa aatattaaaa cgtttctctt
     1501 catgatgatt ctcgtcataa gcatggcttg ccgcgagggt atagcttatc cttcgcaggt
     1561 tgttggaata actcaactga taatccttac tgctgccgct acgcccccag taatctcgcc
     1621 ataacgtact taaggacaca gaaccccagc cttctggcaa tgactgactc atattggcag
     1681 agaagctatt tttacggcca aaatcgttct ggtaataatc ggcaatgtca tagatatcgt
     1741 tttcatcacg gcgataatta tctttattgt ttgcccatac gtgatcatta aatgtccgat
     1801 aatcacgcga cgaataacgc caggccgcca gaccaaaacg tgtcgacgtt tggctcacaa
     1861 atttgttgta ggcaatttga taactttgcc cgtcaaacac atcgccgttg tcttgtttac
     1921 tatgcgactt cgtggcatcg acggaaatgg caccaatgcg tgtattccag ccagtcccca
     1981 aagtgaacgc gtaataatta ttcgcgacca tcgagccacc atacagcgtt aataagttat
     2041 taaaaccata ctgatgaccc acctggacaa aatcactttg tttgctcgcc ccttcaatat
     2101 ggctacgacc cgccgcaaaa tcatatttcg acacgccggg ttgcagcata tttggtaccg
     2161 ctgcataagg caccagatag gtggttaccg aaccgtccgc ctctttcacg ctgacatcaa
     2221 gatccgctcc accaccggcc aactgcaaat ctgtaatcgc aaacgggcca ggaggaacct
     2281 ctttctgata aaccacaaaa ccattctgtt caatagttac cagcgcgtta ctctgagcaa
     2341 tcccctgcac ccgtggcgta aaattttgtt tcgagttagg caacatctgc atatcacgaa
     2401 acaaccgcac accgctgaag cgaacagaat caaaaatatc gcttgatgtg tacatatcac
     2461 ccacacgaag cgtgccgaga agttgggcaa atccacgttc cagatacagg gtattgcttt
     2521 tccacacccc tggattgtta tttgttttac tgaaactggc atcagaatgc agttgccacc
     2581 ccaataaatt taacccgctg ttaaaacgta catatgtact cttgctatta ccggacgctt
     2641 tataatcgct gtaatactga ctcatataat aagaggtata aaacgcatta ataccccgct
     2701 cccagttttc aggtggtaca tagccacttt ccagttcttc cacccaggcc tgcgggacac
     2761 tgaaatcgag acgaaaaact ccgatatccc aggtatagct cccgccctga ataagttgct
     2821 taaatgttaa acattgctta ccgctggcaa agctatcggt attaatgcct aaccgcttga
     2881 tcatttccct tgataaacat gtttcttgcg ggttgtcttt aacaatgatc tcatatttcc
     2941 cacgccattg cttattgaca taaatatcga tgtcatactg ccccggcaag ggctgattat
     3001 cttcaagacg aatatttgaa acctgctggt ctttcattcc acctatcatg aaatgggtat
     3061 caaaggtttc ttcagctgca taagcttcaa tgccgatcaa taacgctacg attgctgatg
     3121 caagtggggt cattctcaac ataggcagct cctgcaatta aattttgtca ctaatatagt
     3181 tgccatggtc atcgataatg gtcagatgcc agttatttgc attattactt ttgacattaa
     3241 cactctgact ttctaagggg gcaatcataa tagtttcata attgactttg acattattag
     3301 ctttgacatc cgaaatcgtc acccaattag ctgagtcatt ttttatcacc aatccattgc
     3361 cactgcgatt taccagtaac tttttaaagg tcactttatt taccggagca atacccgctg
     3421 gccggtaaaa caacttaatt ctgttttgca tcgcaaactt cagtgcattc ttaccttctt
     3481 gttctggact atttggtgga atatccagaa cattcagata aaaaatactc tctttattag
     3541 tggggagttt attcggcata attttaattt ttacttgctg cccggaattt gcccctattt
     3601 ttgccactgg tggcgttaac atgaaaggaa cctgaatttt ttctggtggt aatgacgtat
     3661 cgccatcatc aatccacgcc tgcatcagcg aagaacggtt tccctggttc atcaactgca
     3721 ccatcacttc tttgtgctct gccgggtaaa taatacgcgt cccgtagata acaataccgg
     3781 catgtgcagg aaggaccata gaagaaatga gtaaagctaa taatcctttc atcttaatgc
     3841 ctcttaaatt aaaaggccgc catggagata gcggccatgt taattacctg aagaatcttg
     3901 caattttatt tattaatcct gcgccacacg cataatcagg gaagataaca catcacctga
     3961 attcactgag ttatctgtca gcttcaggta ttttgcttta aacggaatag catattgacc
     4021 ttccgctgtt ttataactct caatgttaaa aggtgcagaa acatccagga catcgctgtt
     4081 atcacctaaa tagagatagt accccacggt atcgttgcta ctggctatcg ccttatcgtt
     4141 tccggttatt ttatcgttgg taatcaacga gagttttaca ccagcaactt ctttcggaca
     4201 gctaattttt aacatgctgc tggttacacc gactgcacca tcataggtcg agtatttagc
     4261 tttaccttca aagaccgtag ttggtacagt tggcatatta atacttactg aatcgccacc
     4321 tgcttcgaat ttacacggag attctgcaat attcccttta atggttaatg ttccggtatc
     4381 aacgtctgcg gcaaataccc cagcgctcat aaaaaaagaa gaaaaaacag cggcagcaat
     4441 aattgaacgt ttcataatat gctccttaat aaaatctatt tttggttgtt aagagtctgt
     4501 atctcttgga aacaaatttc tcacaccaaa aaagttgccg caatgtaaac acattgtttt
     4561 attgatttaa agc
//
LOCUS       DADTFG010000230         4569 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.231, whole genome shotgun sequence.
ACCESSION   DADTFG010000230 DADTFG010000000
VERSION     DADTFG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.231"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(23..406)
                     /gene="quuD"
                     /locus_tag="J1138_004454"
     CDS             complement(23..406)
                     /gene="quuD"
                     /locus_tag="J1138_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HBA8456147.1"
                     /translation="MRDVQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVCGMTFMSLASKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLDMDIVANNSN"
     gene            complement(492..632)
                     /locus_tag="J1138_004455"
     CDS             complement(492..632)
                     /locus_tag="J1138_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBA8456148.1"
                     /translation="MMFEFYMAERLRHRWMRLRLYRFPGSVLADYRILKNYAKTLKGA
                     CV"
     gene            complement(629..991)
                     /gene="rusA"
                     /locus_tag="J1138_004456"
     CDS             complement(629..991)
                     /gene="rusA"
                     /locus_tag="J1138_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBA8456149.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMSVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDVQVVDYRVVKM
                     PVTKGGKLELTITEMGNE"
     gene            complement(988..1278)
                     /locus_tag="J1138_004457"
     CDS             complement(988..1278)
                     /locus_tag="J1138_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBA8456150.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTNFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(1271..1441)
                     /locus_tag="J1138_004458"
     CDS             complement(1271..1441)
                     /locus_tag="J1138_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456151.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1441..1896)
                     /gene="ybcN"
                     /locus_tag="J1138_004459"
     CDS             complement(1441..1896)
                     /gene="ybcN"
                     /locus_tag="J1138_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HBA8456152.1"
                     /translation="MNLSQDGIKLHRGNFTAIGQQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(1893..1994)
                     /locus_tag="J1138_004460"
     CDS             complement(1893..1994)
                     /locus_tag="J1138_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456153.1"
                     /translation="MRTHNPNSLLPSQMQKCTCNSLHLTFDLCGGEA"
     gene            complement(2107..2688)
                     /locus_tag="J1138_004461"
     CDS             complement(2107..2688)
                     /locus_tag="J1138_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015366251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456154.1"
                     /translation="MKIDHDFGYFLDPNKMASIANYEDESAALLKLMLIHEDFLRVVI
                     ENLRPDGSEKYNSIRSYKYFASKLSAAVLLGLPVSLADAMAELNTIRNKYAHKIEYVI
                     TNEEVDKLAESIKNIKLEEINHGGYVHQVVEPILKDGIYAIALMKELPPDLEENRIRI
                     CKLVTSAHCMCLLGAFWLINTLHVKGKLKLSKA"
     gene            complement(2736..2985)
                     /locus_tag="J1138_004462"
                     /pseudo
     CDS             complement(2736..2985)
                     /locus_tag="J1138_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
     gene            complement(3020..3220)
                     /locus_tag="J1138_004463"
     CDS             complement(3020..3220)
                     /locus_tag="J1138_004463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456155.1"
                     /translation="MMYSVTFDETGRVEDIELEREVRAGDRLSLNIDGLDGIYIVMTV
                     SGPIYENVCVPMNIRVQKYWKQ"
     gene            complement(3383..3991)
                     /locus_tag="J1138_004464"
     CDS             complement(3383..3991)
                     /locus_tag="J1138_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456156.1"
                     /translation="MTTQLAFHKTTFTPICHNNRIWLTATEVGLALEYADDKAVQRIY
                     SRHSDEFTDMMTRVVKVTTPRGMQESRVFSLRGAHLIAMFARTPVAKEFRRWVLDILD
                     REIQQSPITKQFTDNELCTLAWLWRAGDMMLTACQNVTPLLQVAEHREAGRFTSIGQE
                     YPQILHKAREILARETAHVKLQPWQDNKWSRVLPHLRQDRLQ"
BASE COUNT         1231 a         1112 c          977 g         1249 t
ORIGIN      
        1 acttttagta aacaattgca tatcaattag aattattagc aacgatatcc atatctaacc
       61 ggatatctaa tgccattaac atcccttcaa ttatgccctc agccttctgt aaccttttcc
      121 cgatataacc atccgagcag caatgcttac ttgccagtga catgaatgtc ataccacata
      181 cataataatc tactaataaa tcgtgtaaat cgctgttgtt ctttttcaga cgggccatgc
      241 acccgcaaat gatcatcgcg tcatcgtcac aacattgcgg gcgagatttt acttttgaag
      301 gaattaatcc cttaaaaccg gcagcaatgg acgaccaggt cacatcttca tgattattag
      361 ctgcccacgc cccccaacgc tcaagaacca tctgaacatc acgcatcaac ttactccaca
      421 aaaatcaggc cagaactcca attaaaagca aaaaacaaca aaacagtatt agtcgattgt
      481 tatctctgac ttcatacaca tgctcctttc agtgttttgg cgtaattctt cagtatccgg
      541 taatcggcca aaacagaacc aggaaaacga tataagcgca ggcgcatcca gcggtggcga
      601 agacgttctg ccatataaaa ctcaaacatc attcattccc catttcggtg atggtcagct
      661 ccagcttccc acctttggta acgggcatct tcacaacacg gtaatcaacg acctgaacat
      721 catccagcca gaaacctgct ttggtgagtg cgtcaaaagc ggctttttgc agattatcca
      781 ggtcacggcg acggcgatcc ggcatgtggc actcaatacg gattttcact gacatagcca
      841 ggccgatatc cagcatggag cctttgatga ttcgggcgac gttatcgcgg tatgcctgcc
      901 cctctgcgct gatgtgcgtg cgcccgcgat tatggcggta atagcgatta ttgctcggag
      961 gccagggtaa tgtgatgctg taagtattca cgccttaatt accccctctt tcagccagat
     1021 aacctgcgtt ctcgccatac cttccagcgc gcattctttt gcatatccag cgtcaacaaa
     1081 attcgtgcga cggtcgatct catcgtgaca ggcagaacat gcaatggtgg caatcaggtc
     1141 tggcggttta ataccggtgc cgcacaatcc agccagccgg atatgtgcca gtacagacgt
     1201 ttcagggttg ccattacata cgccagggat tcttacctgg cattcccgac cacgcgctgc
     1261 ttttctcaaa tcagccatga ttcctccttg ctgccagtcg caaccatttt ttatcaacca
     1321 ggctggcggt atatccgagc agtgttggta tttcggatgg tttcagctca ggcttacgct
     1381 tacgacgatt tggtactctg tagatgtgtc cgttcatgac acgaataagc ggtgtagcca
     1441 ttacgcctcc tgcttgtcgc gcagcagctg gaactcgcag ctctgcggaa tagtcaggtg
     1501 gcagccaata ttcaccgccc aggcttcaac cttacacagg aagacataca tctctccggt
     1561 atcgagatcg gaggtatggc gtaacgactg gatagtagtg atttcgccgg ttacgacatc
     1621 aaccaggtcc ttggtttcat aaccgaggta tgtgtgtttg agagcatctt ttacccatgc
     1681 tgcggtagcg aacgatttcc ccctgctgat gaggtattca ctgatttcgc tgtaccacat
     1741 gtggctgagt gcattctggg aaagactgcg tttctcacgc cacggtttaa gcaccatgcg
     1801 aaagcatttg ccttcctcca gataaggctg gatctgctgg ccgatagcgg tgaagttgcc
     1861 gcgatgtaat ttgatgccgt cttgtgagag gttcacgctt cacctccgca gaggtcaaac
     1921 gttagatgca aagaattgca ggtgcatttc tgcatctgtg aagggagaag agagtttgga
     1981 ttgtgtgtgc gcataaacgt ccccgtttag cgcagaagtc accgaagttg ttcaggctcc
     2041 ggtgatacaa ttatggcgaa ttgattacgg agaatcaatt ttgcgaaaat gacacgattg
     2101 atctactcag gccttggaaa gcttgagctt accctttacg tgcagagtgt taattaacca
     2161 gaaggccccg agcaaacaca tacaatgcgc gctagtgact aacttacaaa tacgaatgcg
     2221 attttcttca agatcaggag gtagctcttt catcaacgct atagcgtata ttccatcctt
     2281 gagaattggc tcaacaactt gatgcacgta gccaccatgg ttaatctctt ctaactttat
     2341 gtttttaatc gattctgcga gtttatcaac ctcttcattt gtaattacat attcaatttt
     2401 atgagcgtac ttgttacgta ttgtgttgag ctcagccata gcgtctgcaa gcgagacagg
     2461 cagccctaac agaactgcgg cagacagctt tgacgcaaaa tacttatagc ttcttatcga
     2521 attgtatttt tcagagccat caggtcgtaa gttttcaata acaacccgaa gaaagtcctc
     2581 atgaattagc atcaatttta atagcgcggc agactcatcc tcatagttag ctatcgaggc
     2641 cattttatta ggatctagaa aatagccaaa gtcatgatcg attttcacgt aattaccctc
     2701 atccgttgtt gggctttgtt caattcgaga gaatttcatt tatcttcctc ctgtttcata
     2761 tatctagggt cagatgtttt aggctgggtg atgctatgct caatggagaa gtcagacgcg
     2821 ctccatgaag taatcacaaa gatgctctgg ttgctggcac atcaccactt cggctatgac
     2881 gagaattttg gagcgttctt tatatgcaac accagacgca gcaaggtcaa agttaacatt
     2941 gtcacgttcc tcttgcggtt ttgctgcaat gttccaatct gacataaaaa tcccctcgat
     3001 catctgaggg gattatacat cattgcttcc aatacttttg aacccggata ttcattggca
     3061 cacaaacatt ttcatagata gggccgctaa cagtcatgac tatataaata ccatcaagac
     3121 catcaatatt aagcgagagt ctatctccgg ctctaacctc acgctctagt tcaatatctt
     3181 ctactcgtcc agtttcatcg aaagttactg aatacatcat ttcaaatttc catctaagcg
     3241 tatgagatgt ctaacgaaaa taccataaag agaatttgat tacattgaac tttttcagtg
     3301 cttacttggc ttatctgaag ccttaatcat tgccgcccag tgcgctatct gattgcatcc
     3361 attcagcgcc taatatgtaa aactattgca atctatcctg acgcaaatgt ggcaacaccc
     3421 tgctccactt attatcctgc caaggctgga gtttcacatg tgccgtttct ctggcaagga
     3481 tttcgcgcgc cttatgtagt atctggggat attcttgccc gatagaagta aagcgaccag
     3541 cttcgcggtg ctccgcaacc tgaagaagtg gagtaacgtt ctggcaggca gttaacatca
     3601 tatcccctgc tcgccataac caagcaagtg tgcaaagttc gttatcagtg aattgttttg
     3661 tgattgggga ttgttgaatt tctcgatcga gaatatccag cacccagcgg cggaattctt
     3721 tggccacagg agtacgagca aacatggcga tcagatgggc tccgcgaagg ctaaatactc
     3781 gagactcctg cattccacga ggggtggtca ctttgaccac ccttgtcatc atatctgtaa
     3841 attcatctga gtgacgagag taaatacgct gaactgcttt atcgtccgca tattccagag
     3901 ctaaaccaac ttcagtggca gtaagccaaa ttctgttatt gtggcaaatc ggggtaaacg
     3961 tcgttttgtg gaatgctaat tgagtagtca tagtatcacc tcatcaggtt aaccatcacc
     4021 accaacgacg ccaatcgact ggtggtgaac tgtgcaaggt tggcgtaacc ggctactcaa
     4081 acccggcgct cccgaagaag ccctcacaca gcccaccata atttgggcgt agccgtgctt
     4141 agcgcataaa aaaaccgctt aacgcggcat gcgttgagta gtattccggg acgccaatcc
     4201 cgtgtgccga ttttgcggca actcgtaaga atatatcccc agataaatca tgtcgtcaac
     4261 cccagtaaat ggacaccacg atgatcatag ccatagtgac tacaacagtt acaattgctt
     4321 caggccaaga cataacgatt tccatatcac cattagccgc cagacgaata gatactctca
     4381 gttctggctg ttgaggtaaa acaccgaata caaaaaacac aggtgcattt ctgcatctgt
     4441 gacaggagga gagagtttag gttgtatacg cgcataaatg ccccgtttag cgcagaagtc
     4501 accagagttg ttcaggcttc gatgacttaa ttatagttgt ttgattcaaa aaatgaatct
     4561 gataattaa
//
LOCUS       DADTFG010000231         4448 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.232, whole genome shotgun sequence.
ACCESSION   DADTFG010000231 DADTFG010000000
VERSION     DADTFG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.232"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            616..1581
                     /gene="lipA"
                     /locus_tag="J1138_004465"
     CDS             616..1581
                     /gene="lipA"
                     /locus_tag="J1138_004465"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBA8456157.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1681..1884)
                     /gene="tatE"
                     /locus_tag="J1138_004466"
     CDS             complement(1681..1884)
                     /gene="tatE"
                     /locus_tag="J1138_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBA8456158.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2013..2522)
                     /locus_tag="J1138_004467"
     CDS             complement(2013..2522)
                     /locus_tag="J1138_004467"
                     /inference="COORDINATES: protein motif:HMM:NF012993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456159.1"
                     /translation="MEYAGGATGRKHRRPLCLHLYDAFAIQESRHVDAGDEIAPLLEV
                     EGMKVGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTT
                     CYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNN
                     RRFAPPQLL"
     gene            complement(2408..2797)
                     /locus_tag="J1138_004468"
     CDS             complement(2408..2797)
                     /locus_tag="J1138_004468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA8456160.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMVQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYACISMMHLPFRNHAMLMLVMK"
     gene            2890..3273
                     /gene="crcB"
                     /locus_tag="J1138_004469"
     CDS             2890..3273
                     /gene="crcB"
                     /locus_tag="J1138_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBA8456161.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3327..3536)
                     /gene="cspE"
                     /locus_tag="J1138_004470"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3327..3536)
                     /gene="cspE"
                     /locus_tag="J1138_004470"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBA8456162.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3711..4271)
                     /locus_tag="J1138_004471"
     CDS             complement(3711..4271)
                     /locus_tag="J1138_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HBA8456163.1"
                     /translation="MNVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1199 a         1121 c          978 g         1150 t
ORIGIN      
        1 ttattgtaat ttttttaaat attatttttt catgaatgaa attggagtaa gtgcgtggat
       61 agtaataatc aaattgaacc ctgtttaagc agaaaatcgt cagaaggtaa accacaaata
      121 tttacaacct tacgaaatat tgatcttacc cttctgacta ttctgaagtt gcatatgtcc
      181 ataaaggaat cgttaatgca gcgaaagtgc ttaatctgcc ccccctcggt cggagccctg
      241 gtccttcctg tcatctatcg ggcgattaaa actcactatc cacagctttt actgcgcaac
      301 ccgcccatcg gcggcgcgga aaaccaactc agtcagtttc aaaccgatct catcaccgat
      361 aacatgtttt gcacaggacc atagatgtta tccgtaatgc attttgaaaa agtattgtta
      421 cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
      481 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
      541 aaatagttga taattacaac aatctattga attgaaacgc tttccttcat aattcgcaac
      601 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
      661 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
      721 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
      781 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
      841 ctggcggaat gctttaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
      901 cgccgttgcc cgttctgtga cgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
      961 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
     1021 gttgaccgtg atgacctacg cgatggcggt gcccagcact ttgcggattg cattactgcc
     1081 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgcggtcgt
     1141 atggatcgtg ctctggatat tctgactgca acgccaccag atgtgttcaa ccataacctg
     1201 gaaaacgtac cgcgtattta ccgtcaggta cggcctggcg cagattacaa ctggtcgctt
     1261 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc caaccaagtc tggtctgatg
     1321 gtgggactgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
     1381 ggtgtgacga tgctaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
     1441 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
     1501 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
     1561 aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
     1621 gctttttgaa gcgggttttt gtatcgaaca agatattgag ggagcgtcct gctcgccacg
     1681 tcactcttta tgagagagct tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc
     1741 agcatcgtca tcattcatcg ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc
     1801 cagcgtacgt aacttcttag tcccaaacag cagaacgacc agcgccgcaa ctaccagcag
     1861 tttggtaata ctaatctcac ccatagatac cttcttgaca tataaccggc aacagaacgc
     1921 gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc gcacacaata gcaatctgta
     1981 acaaggtgaa tcaagcgccg agtaaaaaaa catcataata attgtggcgg cgcaaagcga
     2041 cggttgttta agacgggcag ttgcgcgcgt acctgacgca cacgttcggg cgtcacttcc
     2101 gccataatga gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa gggatctata
     2161 atccggcttt gcccgatatt tttgttcccg cactcccccg ccgccaccat ataacaggtg
     2221 gtatccagcg cacgagcggc aagcaacgtt gaccagtgat gctctttgag cggcccacga
     2281 acccaggcgg caggaagtac caggatttca gctccctgta atgcctgtgc cagcgccagc
     2341 tctggaaagc gtaagtcata acaggtcatc agaccgacct tcatcccttc cacctccagt
     2401 aacggagcta tttcatcacc agcatcaaca tggcgtgatt cctgaatggc aaatgcatca
     2461 tagagatgca ggcataacgg gcgacgatgt ttcctgcctg tagcgccacc agcatattcc
     2521 atgcacgccc cggtgttgaa ggaacatgaa tcgtcagaat tgtcgtcatc atgttacgtt
     2581 tactttctcg ccgtaaacgg ccgaggaatt cgccttccag cagctgtgct gatttaaccg
     2641 aaagatccgc atcatgatca tcacgcgcca gtaatgcttc cggcaggaca aacagcgaaa
     2701 cctcgttttc cgccgcctgc accatcaacg aggcacaaat ctcagcgttc ttttcccaca
     2761 cagatgtaac agcaaactgt cctgcggcaa ccagcattgc cacctcctgt cagagtcgta
     2821 aaattcgcca acggcgatta ttcggcgtta cacttatcac tcatacaaat caaatagcag
     2881 gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac gggaagcgtg
     2941 gcgagatggc tgttaagtat gcgatttaac ccgctgcatc aggcgattcc gttggggacg
     3001 ctggcagcaa acctgattgg ggcattcatc ataggaatgg gattcgcctg gttcagcagg
     3061 atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg tggcggtcta
     3121 acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg ccgctttggc
     3181 tgggcgttac tgaacgtttt cgtcaatctt ttggggtctt ttgccatgac cgcactggct
     3241 ttctggctgt tttcggcctc aaccgcacac taaagaccaa aaaaaacccg ctgattaagc
     3301 gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc agaagggcct
     3361 ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt tttaaaacca
     3421 ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg
     3481 aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt agacatcaaa
     3541 attaccttta catgaaaaat agacacaaat gctgtgtcgg ttaccagtac accaattgtg
     3601 gtacgctttg tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta tttttgatgt
     3661 taattatttt actggttgat tttaaaggat ttactaaaac ttcatttgtc tcaaaactga
     3721 aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat gtaggtcatc
     3781 tgaaacgtcg cggggccata ccccacagag gccaatggca gtagaaccgg aagagggatg
     3841 taattccagt tatcgcgcgc cgttacgcca gcggtaaatc ccaggcctaa atgaaaattt
     3901 tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg ttcccatttg
     3961 ttccacgagt ccttaaatgc catggcatac aggccatgcc agtttccttt ttcatcccaa
     4021 cgcgactgac caaaaccgcc gccccacggt cgctcgttat agcgatcagt tttttctttg
     4081 tcgtaagcaa aacgtgcatg ccaggtgatg gcaggaatat ataaatcata atgttctggc
     4141 tgttgccagg tttgtgcaat attttctcta aacgttgtca tccactcatc agcgttagca
     4201 aaaactttac caacactgat taactgaata aaaacaaaga aaaatatagc gacatattta
     4261 ctcacgttca tttgtgacca ttaaacattt atcaaaaatc tactactaac atagcaaagc
     4321 tacaattaac ataacctcaa tagtcccaac atgaagaata atatgaatag ctgccctatt
     4381 tatttccgct agttcaagat aaatatttcc gcgcatcgat taaagacgac gcatcgtttt
     4441 ctgcgatg
//
LOCUS       DADTFG010000232         4298 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.233, whole genome shotgun sequence.
ACCESSION   DADTFG010000232 DADTFG010000000
VERSION     DADTFG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.233"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            41..835
                     /locus_tag="J1138_004472"
     CDS             41..835
                     /locus_tag="J1138_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8456164.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            989..1750
                     /locus_tag="J1138_004473"
     CDS             989..1750
                     /locus_tag="J1138_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456165.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHWP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISVANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPYSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1792..2874
                     /locus_tag="J1138_004474"
                     /pseudo
     CDS             1792..2874
                     /locus_tag="J1138_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2896..4089
                     /locus_tag="J1138_004475"
     CDS             2896..4089
                     /locus_tag="J1138_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA8456166.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLSVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
BASE COUNT         1173 a          932 c         1088 g         1105 t
ORIGIN      
        1 aaaatgtcag gttttgtagc aatggcgtgg acgagtctga atgaagaagc ctctggttat
       61 tatcagtgcc tgtcagttca ccaggttggc actggagtct ctcatcccaa cggacaggta
      121 tatcgtgcgg gtgtacagca acgtcactac agaggttgaa ttcgttttaa agacctcctg
      181 tggctacctg ttagctgata tcccttcgct tccttcaggg gagttggttc ttctggcttg
      241 tctcgcccgt ttgcggagtg atgccgggca atggcaggtc tgcctgaccg gtgactccga
      301 ctggttcgat aatgctcctg ctgcaagtct gtataacggc ttcccacgca tcggcaccat
      361 gctgacagcg agtcgttatc agaatcaaat tatccgctgg ttactgcgtc ctttgcccgg
      421 cgatatgagc gatttctggc tcctgactgc acgggagttg agcgtgctta agattctgat
      481 gcagggtatg agtttcagtg agattgcccg ttatgaacaa cgcagttcaa agaccctgca
      541 tgcgattgcc acacgggctt taatgaaact tggactggga acactgagcg atttcaggct
      601 gctctatacg ggatgcggtg acagcagagt gaaacggaat atacggctac atgcaaaata
      661 tatcggacaa cattccgctc atcgactcac tcagtatgtg cagaacatgg tatccggaat
      721 tctttatcca gagaaagttg ccgttggtga atcaataaat tctgacgcca tatccggaaa
      781 agtactttcc ggacaaagaa aaaatatgtc taaaaaaaga ccaggattgt tatgacacag
      841 cagcgttgtt actacccccc actgcggcgc atacggactc gctacagtgt aggagccaaa
      901 atatttctac tgctctgggc tgcaggattg ggaggcacgc tgtacagcct gtacagcgtg
      961 ctggcgtact gacatctgtg agggaaggat gcgtttcgtt cttttctgtc cctcaggaat
     1021 tgtaccagcg cagttcgcag ctctatcaac cggttctgtc ggcaatgtta cctgcatcgg
     1081 aacagaagag gagttacgca ataaattgcg gcactggcca cagagtgtcg tgatatcggc
     1141 agggcgacct gccgaatgcg ctgaaatgtg gttccgattt tatcgggatc actcattcgt
     1201 ggtggtattg tgcgtcgcgc cgttttttct gccaccggat gtgagtattt ctggcgttct
     1261 caaaaacctg aggttactga aaccaggaat gtcagtagag cgtgttatca gtgttgcaaa
     1321 tgcctctggt gggttttcag ggctgaaaca tgcagaaatt ttgccagtta tggacagtta
     1381 ctctgtcttt atgaaagagg tgaataaccg cacaaagaca atcgtcatgt cagaacgctt
     1441 cccggaaaag caaaagaagg tgttgtcgtt gcttcttgcc ggacacagct gggaatacag
     1501 tgcgcagttt ctcaaaacgg ggataagaca gatatggctt gccgagcagt cgctaaaaaa
     1561 gcgttgggga ataccgtaca gcatgtcatt gcgggaagcc ctgctgctga gcagcaataa
     1621 atttcacggt gatggaaatg cattggaaaa aacgaatgcc atgacattgc gtgaaaacgg
     1681 aaataccaac aggtattccg tggtggtaaa tgcagggacg caggctttat ctctgcataa
     1741 atataaataa gtggaaaatt gaaattagtt accagaatgg agaatttctt catgaaaaac
     1801 aataaggcac tttaccgcag cgcattagcg acagctattg ttatggctct ttctgcacca
     1861 gcattcgctg ctgataacgc ggtatcaact gattcggtta cgctgaataa agataagacg
     1921 actctggatc aagatgttgt tattaacaat acagcagaca aacagattac agccgtaaca
     1981 attaatgcgg cagatgaaga ccttaatgtt gattttgctg gtcatgatat tactgctgaa
     2041 tcaacggcag acaacaaatt cgctgaaggt gtaaaagtta gcggtgacaa aaatgttgtg
     2101 attaatgcta caggctccac catcacagct caaggtgaag gcacctatgt ccggactgca
     2161 atggtcattg attcaactgg cgatgttgtt gttaatggcg gtaatttcgt tacaaaaaat
     2221 gaaaaaggta gtgcgacagg gatatctctg gaaggggccc ggggaaataa tgtcacgctt
     2281 aatggcacaa ccataaatgc tcaaggtaat aagagttcca gcaacgcctc cacggcaatt
     2341 tttgctcaaa agggtagttt attgcagggt tttgacggtg atgcaaccga caacatcacc
     2401 cttgctgact caaatattat taatggcggg attgaaacaa tagttactgc cgggaataag
     2461 acgggaattc atacagtcaa cctgaatatt aaggatggct cagtaattgg ggcggctaat
     2521 aataaacaaa caatttatgc ctctgcttcg gcacaaggcg caggttcagc aacgcaaaat
     2581 ttaaatttgt ctgttgctga ttcaaccatc tactctgatg tcctggccct ttctgaaagc
     2641 gagaattcag ccagtaccac aacaaatgta aatatgaacg ttgcccgctc ttactgggaa
     2701 ggtaatgctt atacgctcaa tagcggcgat aaagcgggta gtgatctgga tataaatctt
     2761 tccgatagtt cagtctggaa aggcaaagtt tcaggggcag gagatgccag tgtatctctg
     2821 caaaacgggt ctgtctggaa tgttacgggt tcctcaactg ttgatgctct ggcagtaaaa
     2881 gacagtacgg ttaatatcac gaaggctaca gtcaatactg gcacgtttgc ttctcaaaac
     2941 ggcactctga ttgttgatgc ctcttctgaa aacaccctgg atatcagcgg taaagcgagc
     3001 ggtgacttga gtgtttacag tgcgggttca ttggatctta tcaatgaaca aacggcattt
     3061 atttctaccg gcaaagacag cactctaaaa gccacaggca caacggaagg tggtctgtat
     3121 caatatgacc tgacacaggg agctgatggt aacttttatt tcgtaaaaaa cacgcataaa
     3181 gcatccaacg ccagctccgt gattcaggca atggcagctg ctccggctaa cgtcgctaat
     3241 ctgcaggctg acacgctctc cgcccgtcag gatgctgtcc gtctgagcga aaatgacaag
     3301 ggtggcgtat ggattcagta ctttggcggt aaacagaaac ataccaccgc gggaaatgca
     3361 tcctatgacc tggatgtaaa tggtgtaatg ctgggtggtg atacccgctt catgactgaa
     3421 gatggtagct ggctggccgg tgtggcgatg tcttctgcga aaggtgacat gactaccatg
     3481 cagagcaaag gtgacactga aggttacagc ttccacgctt acctgagccg ccagtataac
     3541 aacggtatct tcattgatac tgctgcacag tttggtcact acagcaacac ggcagatgtt
     3601 cgcctgatga atggtggcgg taccatcaaa gctgacttta acaccaatgg ttttggtgcg
     3661 atggttaaag gcggttacac atggaaagac ggtaatggcc tgtttattca gccatatgcc
     3721 aaactgtctg ctctgactct ggaaggtgtg gattatcaac tcaacggcgt ggacgttcat
     3781 tctgacagct ataactctgt gctgggtgag gccggtacgc gcgtgggtta tgacttcgct
     3841 gtgggcaacg cgaccgttaa accttatctg aatctggccg cactgaacga attctctgat
     3901 ggcaacaaag tccgtctggg tgatgagtct gtcaatgcca gcattgacgg tgcagcattc
     3961 cgcgtgggtg caggtgtaca ggctgatatc accaaaaaca tgggagcata tgcaagcctt
     4021 gactacacca aaggtgacga cattgagaac ccgctacagg gtgtagttgg tatcaatgtg
     4081 acctggtaag aataaagcag tcggttcccc tgtcgactgt tataccccct gcaggcagca
     4141 gtatgctgcc tgcaggctat aaatatccag tactcatgat gctggctttt tgctgatatt
     4201 taccctgaac attcctgata cagactatta atacaatctt atctgcgaca gatttcattt
     4261 tctggatata ttatgaatga acaaatcaaa caggatat
//
LOCUS       DADTFG010000233         4291 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.234, whole genome shotgun sequence.
ACCESSION   DADTFG010000233 DADTFG010000000
VERSION     DADTFG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.234"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..284)
                     /locus_tag="J1138_004476"
     CDS             complement(<1..284)
                     /locus_tag="J1138_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA8456167.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITG"
     gene            complement(399..474)
                     /locus_tag="J1138_004477"
     tRNA            complement(399..474)
                     /locus_tag="J1138_004477"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Thr,seq:ggt)
     gene            complement(481..555)
                     /locus_tag="J1138_004478"
     tRNA            complement(481..555)
                     /locus_tag="J1138_004478"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(520..522),aa:Gly,seq:tcc)
     gene            complement(672..756)
                     /locus_tag="J1138_004479"
     tRNA            complement(672..756)
                     /locus_tag="J1138_004479"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(720..722),aa:Tyr,seq:gta)
     gene            complement(765..840)
                     /locus_tag="J1138_004480"
     tRNA            complement(765..840)
                     /locus_tag="J1138_004480"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(805..807),aa:Thr,seq:tgt)
     gene            1202..2152
                     /gene="coaA"
                     /locus_tag="J1138_004481"
                     /gene_synonym="panK"
     CDS             1202..2152
                     /gene="coaA"
                     /locus_tag="J1138_004481"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBA8456168.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2181..3146)
                     /gene="birA"
                     /locus_tag="J1138_004482"
     CDS             complement(2181..3146)
                     /gene="birA"
                     /locus_tag="J1138_004482"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBA8456169.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3143..4171)
                     /gene="murB"
                     /locus_tag="J1138_004483"
     CDS             complement(3143..4171)
                     /gene="murB"
                     /locus_tag="J1138_004483"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBA8456170.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1112 a         1058 c          968 g         1153 t
ORIGIN      
        1 ccggtgatca tgtttttaac atagtcggcg tgccccgggc agtctacgtg tgcgtagtga
       61 cgggtcgggg tgtcatattc aacgtgagaa gtgttgatgg tgataccacg agctttttct
      121 tccggcgcgt tatcgatctg gtcgaatgcg cgagcagcac cgccgtaggt tttagccagt
      181 acggtagtga ttgcagcggt cagcgttgtt ttaccatggt caacgtggcc gatagtaccg
      241 acgttaacgt gcggttttgt acgttcaaac ttttctttag acatcgattg tccctctaag
      301 acacggataa atcggtgata tcaccacatc aaccaggcaa catgcccgac ttgttgaatg
      361 caataaacag aaggaaaaaa cagggaggag aaaagaagtg gtgctgatag gcagattcga
      421 actgccgacc tcacccttac caagggtgcg ctctaccaac tgagctatat cagcacatct
      481 tggagcgggc agcgggaatc gaacccgcat catcagcttg gaaggctgag gtaatagcca
      541 ttatacgatg cccgcatcct ggaactcggc tacctgattt tcattctgca ctgaatatcg
      601 agagaagctc tctttattcg agccggtaag cgaacttatc gtctcgggct acgccatcgc
      661 gtggccgaaa ttggtggtgg gggaaggatt cgaaccttcg aagtctgtga cggcagattt
      721 acagtctgct ccctttggcc gctcgggaac cccaccggac ttgatggtgc cgactaccgg
      781 aatcgaactg gtgacctact gattacaagt cagttgctct acctactgag ctaagtcggc
      841 atcaagtagc gcgcattcta tggagacatg cgagttcatg caactaaaaa attgcataat
      901 ttgttttatt ggtcacattt tatgcgacac gatgaagaaa cagccgaacg gagggcagat
      961 tagcacactt tttcaacatc attgtgctca acaatgtgct cctgctaaac cataattctt
     1021 tttatcagat ggaatatctg tcacattgct tttcaacgat agcttcctgg gagagatttt
     1081 ttcttattat tcctccccat ctggtgttac cctcctgccc attaacccat tcaacagaac
     1141 tgtgacgcgc catggcaaat atcgctttgc cgatagagct atgaccgcca gaaacatgct
     1201 tatgagtata aaagagcaaa cgttaatgac gccttaccta cagtttgacc gcaaccagtg
     1261 ggcagctctg cgtgattccg tacctatgac gttatcggaa gatgagatcg cccgtctcaa
     1321 aggtattaat gaagatctct cgttagaaga agttgccgag atctatttac ctctgtcacg
     1381 tttgctgaac ttctatataa gctcgaatct gcgccgtcag gcagttctgg aacagtttct
     1441 tggtaccaac gggcaacgca ttccttacat tatcagtatt gctggtagtg tcgcggtggg
     1501 gaaaagtaca accgcccgtg tattgcaggc gctattaagc cgttggccgg aacatcgtcg
     1561 tgttgaactg atcactacag atggcttcct tcaccctaat caggttctga aagaacgtgg
     1621 tctgatgaag aagaaaggct tcccggaatc gtatgatatg catcgcctgg tgaagtttgt
     1681 ttccgatctc aaatccggcg tgccaaacgt tacagcccct gtttactcgc atcttattta
     1741 tgatgtgatc ccggatggag ataaaactgt tgttcagcct gatattttaa ttcttgaagg
     1801 gttaaatgtc ttacagagcg ggatggatta tccacacgat ccacatcatg tatttgtttc
     1861 tgattttgtc gatttttcga tatatgttga tgcaccggaa gacttacttc agacgtggta
     1921 tatcaaccgt tttctgaaat tccgcgaagg ggcttttaca gacccggatt cctattttca
     1981 taactacgcg aaattaacta aagaagaagc tattaagact gccatgacat tgtggaaaga
     2041 gatcaactgg ctgaacttaa agcaaaatat tctacctact cgcgagcgtg ccagtttaat
     2101 cctgacgaaa agtgctaatc atgcagtaga agaggtcaga ctacgcaaat aatttaaagg
     2161 ggagttaatg ctcccctttc ttatttttct gcactacgca gggatatttc accgcccatc
     2221 cagggtttta ttattccatc ctgctcaagt aataaagcac cctgtttgtc tattccgcgt
     2281 gaaatgccaa atatttcttt atcaccaatg ataagtttca ctgggcgatt aataaaatta
     2341 tccaactttt cccagcgcga aagataaggt gtcaatcctt cttgctcgaa gagttccagc
     2401 gccgcgcgta attcacgtat cagcatggcc gcaagagtat tacgatcgag attgatcccg
     2461 gcttcctgca gcgtgatcca cccttgatta acgacactct cttcaacacg acgcattgcc
     2521 atgttgatcc ctgcaccaat gactatttgc gccgcatcgc cggttttgcc agtcagctcc
     2581 acaagaatgc ctgccagctt gcgatcctgc agatagaggt cattaggcca tttaacacga
     2641 actttatctg cacccagctt gcgtaatact tccgccatca cgataccgat aaccagactt
     2701 aaaccaatcg ccgccgccgg gccctgttcc agacgccaga acatcgacaa atataagttc
     2761 gcgccaaaag gcgaaaacca tttccgacca cgacgaccac ggccagcatg ctggtattct
     2821 gcaacacagg catcgccaga tttaagctct ccgatgcggt ccaggagata ctgattcgtg
     2881 gagtcaataa ctggcagcac ggctacactg ccgtcatcca gctgactcaa tatcttttcg
     2941 gcattaagta actggattgg ctcgggcaag ctgtatcctt tacccggaac agtaaataca
     3001 tcaacgcccc agtcacgcag tgtctgaatg tgtttattaa tagccgcccg gctcattccc
     3061 agcgtttcac ccaactgctc gccagagtga aattcgccat tcgctaacag ggcgatcaat
     3121 ttcagtggca cggtgttatc cttcatgaaa ttgtctccac tgcgctcact tcacctgatg
     3181 caccaataaa gcggacttca ggctcaagcc agacattaaa tttttcaccc actttttgtc
     3241 ttacatgatg cgccagctgt acaacatctt cgctttttgc atcgtgctca tttatgagaa
     3301 ccagcgcctg ttgacggtgt accgcagccc caccaatttg cgtcccttta agctggcact
     3361 gatcaataag ccaacctgca gccagtttta ctgaaccatc cacctggggg taatgtgggg
     3421 ctgttggaaa ttgtgccaac aacgcgttag cagtttcggc agaaacaaca ggatttttga
     3481 agaaactacc cgcattaccg ttcacttttg gatcagggag tttggtggtg cgcatatgac
     3541 acaccgcatc aaatacttgc tgtggcgtta ccgttgaagg atccaggcga gttaagtcac
     3601 catacgtcag tacaggttgc cactcttttg gcagacgcag acctacggct acaatggcga
     3661 agcgatcctg gtattcatgt ttaaaaatac tgtctcgata gccaaaacgg cactctttgg
     3721 cagttaagcg tacttgcttg cctgtcgcca gttcaacaca atcaacataa gcgcaaactc
     3781 gctgtaattc tacgccataa gcaccaatat tctggatagg tgatgagccg atacaaccag
     3841 gaattaatgc cagattttcc agaccaggca taccttcctg caaagtgtat tttaccaggc
     3901 gatgccagtt ttctccggct cctacatgta aataccacgc atcaggttca tcatgaattt
     3961 cgataccttt gatccggttg atgatcaccg tgccgcgata gtcctccaga aaaagtacat
     4021 tacttccttc acccagaata agaacgggtt gtccttctgc ggttgcatgc tgccaggcat
     4081 tgagtaattg ttgttcgtct tcggcacata caatgtgctg agcattatga tcaatgccaa
     4141 atgtgttcca gggttttaag gagtggttca tagctgcttt cctgatgcaa aaacgaggct
     4201 agtttaccgt atctgtgggg ggatggcttg tagatatgac gacaggaaga gtttatagaa
     4261 acgcaaaaag gcccagtctt ccgactgggc t
//
LOCUS       DADTFG010000234         4194 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.235, whole genome shotgun sequence.
ACCESSION   DADTFG010000234 DADTFG010000000
VERSION     DADTFG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.235"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            127..4068
                     /gene="vat"
                     /locus_tag="J1138_004484"
                     /pseudo
     CDS             127..4068
                     /gene="vat"
                     /locus_tag="J1138_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021534952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vacuolating autotransporter toxin Vat"
BASE COUNT         1155 a          964 c         1071 g         1004 t
ORIGIN      
        1 ctagcccgaa gggtatgtaa agggagtacc cgcagaggaa aaagactttc agtacttacc
       61 tctctggcac tatctgcatt actcccaacc gttgctggtg catcaacggt tggtggcaac
      121 aatccttacc agacataccg cgactttgca gaaaacaaag ggcagtttca ggctggcgca
      181 acaaacattc ctatttttaa taataaaggg gaattagtag gacatcttga taaagcgccc
      241 atggttgatt ttagcagtgt gaatgtaagc tcaaatcccg gcgttgcaac attaattaac
      301 ccgcaatata tagccagtgt aaaacataat aaaggatatc agagcgtcag cttcggtgat
      361 ggtcagaaca gttaccatat tgtggatcgt aatgaacaca gttcatctga tctccacaca
      421 ccaagacttg ataagctcgt aactgaggtt gctccggcta ccgtaaccag ctcatcaaca
      481 gctgatatat tgaacccttc aaaatactcg gcattctaca gggctggttc gggaagtcag
      541 tatattcagg atagtcaggg taagcgacat tgggtaacag gtgggtatgg ttatctgaca
      601 ggaggaatac tcccgacatc attcttttat cacggctcag acggcattca gctgtatatg
      661 gggggcaaca tacatgatca tagcatcctg ccctcttttg gagaggccgg cgacagtggt
      721 tctccattat ttggctggaa tacggccaaa gggcagtggg aactggtcgg tgtttactcg
      781 ggagtaggag gggggaccaa tttgatatat tctcttattc ctcagagttt tctctcacag
      841 atctattcag aggataatga cgctcccgtc ttttttaatg cctcatccgg cgcccccctg
      901 caatggaaat ttgacagcag caccggcact ggctctctga aacagggttc cgatgaatat
      961 gccatgcacg ggcaaaaagg ttctgacctg aacgcaggta aaaatctgac attcctggga
     1021 cataatggtc agattgacct ggaaaactct gtcacgcagg gtgccggttc actgacattt
     1081 actgatgact acactgtcac cacttcaaac ggaagtactt ggaccggggc cggtattatt
     1141 gtggacaagg atgcctccgt aaactggcag gttaatggtg tgaaaggtga caacctgcat
     1201 aaaatcggcg aaggaaccct ggttgtacag ggaaccggtg ttaatgaggg cggcctgaaa
     1261 gtcggggatg ggaccgttgt cctcaatcag caggctgaca gttcaggaca cgttcaggca
     1321 ttcagtagcg tgaatattgc cagcggccgc ccgacagtcg tgctggcaga caaccagcag
     1381 gttaatccgg acaatatatc ctggggctac cgggggggtg ttctggatgt taacgggaat
     1441 gacctgacat ttcataagct gaatgccgcc gattatggcg caactctcgg taacagcagt
     1501 gataaaacgg ctaatatcac tctggattat cagacgcatc cggcagacgt aaaagttaat
     1561 gaatggtcat catcaaacag gggaacagta ggttcattat atatttataa taatccctat
     1621 actcataccg tcgattattt tatcctgaaa acaagtagtt atggctggtt ccctaccggt
     1681 caggtcagta acgagcactg ggaatatgtc ggacatgacc agaacagtgc acaggcgctg
     1741 cttgcaaaca gaattaataa taaagggtat ctgtatcatg gcaagttgct gggaaatatt
     1801 aatttctcaa ataaagcaac cccgggtaca accggcgcat tggttatgga cggctcagcg
     1861 aatatgtccg gtacatttac tcaggaaaac ggtcgtctga ccattcaggg ccacccggtt
     1921 atccatgctt caacgtctca gagtattgca aatacagtct cgtctctggg cgacaattcc
     1981 gttctgacac agcccacctc atttacacag gatgactggg agaacaggac gttcagcttt
     2041 ggttcgctcg tgttaaaaga tacagacttt ggtctgggcc gcaatgccac actgaacaca
     2101 accatccagg cagataactc cagcgtcacg ctgggcgaca gtcgggtatt tatcgacaaa
     2161 aaagatggcc agggaacagc ctttaccctt gaagaaggca catctgttgc aactaaagat
     2221 gcagataaaa gtgtcttcaa cggcaccgtc aacctggata atcagtcagt gctgaatatc
     2281 aatgatatat tcaatggcgg aatacaggcg aacaacagta ccgtgaatat ctcctcagac
     2341 agtgccattc tggggaactc aacgctgacc agtaccgccc tgaatctgaa caagggagca
     2401 aatgctctgg ccagtcagag ttttgtttct gacggtccag tgaatatttc tgatgccacc
     2461 ctgagtctga acagccgtcc tgatgaggta tctcacacac ttttacctgt atacgattat
     2521 gccggttcat ggaacctgaa gggagacgat gcccgcctga acgtggggcc gtacagtatg
     2581 ttgtcaggta atatcaatgt tcaggataaa gggactgtca ccctcggagg ggaaggggaa
     2641 ctgagtcctg acctgactct tcagaatcag atgttgtaca gcctgtttaa cgggtaccgc
     2701 aatacctgga gcgggagcct gaatgcaccg gatgccaccg tcagcatgac agacacccag
     2761 tggtcgatga acggaaactc cacggcagga aatatgaaac ttaaccggac aatagtcggt
     2821 tttaacgggg gaacatcatc gttcacgaca ctgacaacag ataatctgga cgcggttcag
     2881 tcagcatttg tcatgcgtac agaccttaac aaggcagaca aactggtgat aaacaagtcg
     2941 gcaacaggtc atgacaacag catctgggtt aacttcctga aaaaaccctc tgacaaggac
     3001 acgcttgata ttccactggt cagcgcacct gaagcgacag ctgataatct gttcagggca
     3061 tcaacacggg ttgtgggatt cagtgatgtc acccccaccc ttagtgtcag aaaagaggac
     3121 gggaaaaaag agtgggtcct cgatggttac cagattgcac gtaacgacgg ccagggtaag
     3181 gctgccgcca cattcatgca catcagctat aacaacttca tcactgaagt taacaacctg
     3241 aacaaacgca tgggcgattt gagggatatt aacggcgaag ccggtacgtg ggtgcgtctg
     3301 ctgaacggtt ccggctctgc tgatggcggt ttcactgacc actataccct gctgcagatg
     3361 ggggctgacc gtaagcacga actgggaagt atggacctgt ttaccggcgt gatggccacc
     3421 tacactgaca cagatgcgtc agcaggcctg tacagcggta aaacaaaatc atggggtggt
     3481 ggtttctatg ccagtggtct gttccggtcc ggcgcttact ttgatttgat tgccaaatat
     3541 attcacaatg aaaacaaata tgacctgaac tttgccggag ctggtaaaca gaacttccgc
     3601 agccattcac tgtatgcagg tgcagaagtc ggataccgtt atcatctgac agatacgacg
     3661 tttgttgaac ctcaggcgga actggtctgg ggaagactgc agggccaaac atttaactgg
     3721 aacgacagtg gaatggatgt ctcaatgcgt cgtaacagcg ttaatcctct ggtaggcaga
     3781 accggcgttg tttccggtaa aaccttcagt ggtaaggact ggagtctgac agcccgtgcc
     3841 ggcctgcatt atgagttcga tctgacggac agtgctgacg ttcacctgaa ggatgcagcg
     3901 ggagaacatc agattaatgg gagaaaagac ggtcgtatgc tttacggtgt ggggttaaat
     3961 gcccggtttg gcgacaatac gcgtctgggg ctggaagttg aacgctctgc attcggtaaa
     4021 tacaacacag atgatgcgat aaacgctaac attcgttatt cattctgatg ctgtgcagca
     4081 aagggactat tgctttgtcc ctttgctgcg attatctgat tacggtacag gctggaactt
     4141 agattttccg gcgttcacga tacttcatgt aacactcagg ttgagtaatc ttca
//
LOCUS       DADTFG010000235         4156 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.236, whole genome shotgun sequence.
ACCESSION   DADTFG010000235 DADTFG010000000
VERSION     DADTFG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.236"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            110..1768
                     /gene="dpiB"
                     /locus_tag="J1138_004485"
     CDS             110..1768
                     /gene="dpiB"
                     /locus_tag="J1138_004485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBA8456171.1"
                     /translation="MLQLKENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1737..2417
                     /gene="dpiA"
                     /locus_tag="J1138_004486"
                     /gene_synonym="citB"
     CDS             1737..2417
                     /gene="dpiA"
                     /locus_tag="J1138_004486"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBA8456172.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2458..3843)
                     /gene="dcuC"
                     /locus_tag="J1138_004487"
     CDS             complement(2458..3843)
                     /gene="dcuC"
                     /locus_tag="J1138_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBA8456173.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1142 a          959 c         1058 g          997 t
ORIGIN      
        1 tgatttaatg aataaaattt gccacgatca tatttaatat ctatgtattt tgattcaaca
       61 ttttaattac atccgtcaaa gaggctcggg acaacccgca aggaaaacaa tgttgcagct
      121 taaagagaat aaacagtttg catttttcca aagactggca tttccgctgc gtatcttttt
      181 gctgattctg gtgttctcaa tatttgtcat tgcagccctg gcgcaatatt ttacagccag
      241 ttttgaggac tatttaacgc ttcatgtgcg cgacatggca atgaatcagg caaaaattat
      301 tgcttctaat gacagtatca tcagtgcagt gaaaacgcgt gactacaaga ggctggcgac
      361 catcgctgac aaattacaaa gagatactga tttcgattat gtggtgattg gcgatcggca
      421 ctcgattcgt ctttaccatc ctaatccgga gaaaattggt tatcctatgc agttcaccaa
      481 accgggcgcg ctggagaaag gggagagcta cttcatcacc gggaaagggt caattggcat
      541 ggcgatgcgt gccaaaacgc caatctttga tgacgatgga aaagtcatcg gcgtggtgtc
      601 gattggctat ctggtgagta aaatcgatag ctggcgggct gagtttttat taccgatggc
      661 aggcgtgttt gtcgtgctgt tagggatttt gatgttgctg tcgtggttcc tggccgcgca
      721 tatccgtcgg cagatgatgg gcatggagcc aaagcaaatc gctcgcgtag tccgtcagca
      781 agaggcgctg tttagttcgg tttatgaagg gctgattgcg gtggatccgc acgggtacat
      841 taccgccatc aatcgtaacg caagaaagat gctggggctg agttcccccg gacggcaatg
      901 gttgggtaaa cccattgctg aagtggtcag gcccgccgat ttctttaccg aacagattga
      961 tgaaaaacgt caggatgtgg tggcgaactt taacggtctg agcgttattg ccaaccggga
     1021 agctattcgt tctggcgatg atttactggg ggccattatt agctttcgta gtaaagacga
     1081 aatttccacc ctcaatgcgc aactgacgca aattaaacaa tacgtcgaga gccttcgtac
     1141 attgcgacac gagcatctca attggatgtc gacgctcaat ggtctgttgc agatgaaaga
     1201 gtatgatcgc gtgctggcga tggtgcaggg ggagtctcag gcccagcaac agcttattga
     1261 cagcctgcgt gaggcgtttg ccgatcgcca ggtggcgggg ctgctttttg gtaaagtgca
     1321 gcgcgcccgc gaactggggc taaaaatggt catcgtcccc ggtagccagc tttcgcaact
     1381 gccgccagga ctggacagca ccgagtttgc agccattgtt ggcaatttac ttgataacgc
     1441 cttcgaagca agcctgcgta gcgatgaagg aaacaagatc gttgaattat tcctcagcga
     1501 tgaaggcgat gatgtggtga ttgaagtcgc cgatcagggc tgcggcgttc cagagtctct
     1561 acgagacaaa atatttgagc agggtgtcag tacgcgtgct gacgagcccg gtgaacatgg
     1621 cattgggttg tacttgattg ccagctacgt aacgcgctgc ggtggtgtta tcactctcga
     1681 agataatgat ccctgcggta ccttattttc aatctatatt ccgaaagtga aacctaatga
     1741 cagctccatt aaccctattg atcgttgagg acgaaacgcc gctggcagag atgcatgcgg
     1801 aatatattcg tcacattccc ggattcagcc agatattact ggcgggaaac ctggcgcagg
     1861 cccgaatgat gatcgagcgt tttaagccgg ggctaatctt gctcgataac tatcttcctg
     1921 acggtagagg gattaattta ctgcatgaac tggtgcaggc gcattatccc ggcgacgtgg
     1981 tgtttaccac tgctgccagc gatatggaaa cggtgtctga agccgtacgt tgtggtgtat
     2041 ttgattatct cattaagccc attgcctatg aacggttggg gcaaacgcta acccgtttcc
     2101 gccagcgtaa acatatgctg gaaagtattg atagcgccag ccagaagcaa attgatgaga
     2161 tgtttaatgc ctatgctcgc ggtgaaccca aagacgaact gccgaccggc attgacccct
     2221 tgacgctaaa cgcggtgcga aaactgttca aagagcctgg tgtgcaacat acggcagaaa
     2281 cggtggcgca ggcactgacc atcagtcgca ccactgctag gcgttatctt gaatattgcg
     2341 ccagccgcca tctgattatt gctgaaattg ttcacggcaa agttggcaga ccgcaacgca
     2401 tataccacag tgggtgacat gataaagccg gaggaaactt gcctccggca ttactattta
     2461 cttgcctgtg accgctgctg ccgttcctgg caccatcagc tctgtagcaa cgataacaat
     2521 caccaaacca acaagcaccg gcaccgaggt gcgttttacg acttcaaacg gcgagatctt
     2581 cgccatcccg gcaaccgcaa cgactacgcc agaaacgggt gatagggtac gacccaggtt
     2641 tgacgcctgc agcatcggga tagtcaaata cgccgggtta atgccggagg agtgcgccag
     2701 tttcgggatc atctcaacaa acgcataaaa cggcgcattg cctgaaccgg tcgtgactgc
     2761 cgccagcatt gtcaggatca ccaataccag catcaggatg atactcgccg aaccaaacga
     2821 ggtagcgata gaaatcagac tttgaataaa gccgatggtg ctaagcccct gagcgaatac
     2881 cccagcggca accagcagca tcaccacgtt agcaaatgca tctgccatac cgcgataagc
     2941 cacttccaga ccagagaaaa ctttctgggt attaaagctg cggatgaact ccagaatgga
     3001 ggcaattagc atacaaatca ccagaatagt gatgatgtgt aattgcggac cccatttgcc
     3061 gtcaaaaatc agtactccga tgatcggcgt gaacggcaaa atggcataaa acgcaggggc
     3121 agtggtggtg atttcactga catctaacat ttcatgagag atgtgctctt ttttatccag
     3181 ataacgttgc cagaagaagt gggcgatcgc catgccgata attgcagcaa ttgagatagg
     3241 cagcgttgtt ttgaaggcga agtcaatcag cgacatttcg gaagcctgcg ccgccagcac
     3301 cacatcccct gaagtcggtg cgagaataat cgccgccggg gaggcacaaa tggcagcagc
     3361 tgcgccacga ctgataccaa cgtttaccat caccggaaac agggttgcca tcagcaaaac
     3421 acccagaccg gttgcggaag agacggccag tgacatcaga caggcaacaa aataggcggc
     3481 aatcatcaga aggtaagggg agttaatata ctgcaatggt tttgacgcca gcttgaccac
     3541 catatcattc gcgccgatat gggtcatgta agcggcaaag ccacacagca tcataatcat
     3601 catgccgagg tcgccgccgc ggctcattag caatatttta acgtattcaa cgatatccgt
     3661 ggcgctgtag cctgttgaag cctggctgga cggtaacact ttgtgcccca taatggcact
     3721 gataatcagc aataacaggc caccgacaaa taacacgcca gtggcagaat accctttaat
     3781 gatgtagcga gctacaccca caataaccac aaccccaata aggagttcaa tgaatgtcag
     3841 cataattttt cctgtctcca ggccccaaag taaataataa aaaattccta aaagttaagg
     3901 aaaaaatatg cccaataaat tggcggtgaa tgctgattaa aatcaagaaa aactgccatt
     3961 aagacattga agttgctgtt tttatgcgta gataacaaaa cgcatattta gcggttggca
     4021 aatatcacat aataatgtgt gtaaattgat ggtggattga tgcaaatttg ttaattgtaa
     4081 ggtgttagct gcggttgtgt gctcaaaaaa taatctttca tgagcatgag ttgacgatac
     4141 ataatatatt tttcgt
//
LOCUS       DADTFG010000236         4154 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.237, whole genome shotgun sequence.
ACCESSION   DADTFG010000236 DADTFG010000000
VERSION     DADTFG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.237"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..533)
                     /locus_tag="J1138_004488"
     CDS             complement(<1..533)
                     /locus_tag="J1138_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA8456174.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDIPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAH"
     gene            complement(1278..1454)
                     /locus_tag="J1138_004489"
     CDS             complement(1278..1454)
                     /locus_tag="J1138_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBA8456175.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTMNK
                     LYDRAPASVWKYVT"
     gene            2090..2368
                     /locus_tag="J1138_004490"
     CDS             2090..2368
                     /locus_tag="J1138_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBA8456176.1"
                     /translation="MPVKVARCFHDHTGWFNNIRRNILMATPVSLMDDQMVDMAFITQ
                     LNGLTDKWFYRLIRDGAFPAPIKLGRSSRWLKSEVEAWLQARIAQPRP"
     gene            2463..3065
                     /locus_tag="J1138_004491"
     CDS             2463..3065
                     /locus_tag="J1138_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456177.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
BASE COUNT          972 a         1116 c         1001 g         1065 t
ORIGIN      
        1 cagtgagctt tggtatgctc cttcgccaga tagtcagcac gttccagcac ttgctgaaag
       61 ccggtgtcat cattacgcgc cagccacacc gccggccagg ccggaaaatg caccagcgtg
      121 gcatggcacc cgacatccac tcccagggca ctgcaccacg cctgtttaat catcccggcc
      181 agagactccg gagcgcggta atcaccggca cgacaccagg tatcccggtt gaccagcagc
      241 atcaggtgat agtgtttttt acctttgcac tccccaaact cccgggccca gacgtaatgc
      301 agggtggtgg gatgcacgcg tttaccttcc cgatgcttac gtttctggta agcgtcgatt
      361 cgggctttca gggcattgat gaagcgggat atcacagctg cgtccgttgc tgccggtata
      421 tccgggagac gcaggtcaac ccgcaatgcc gtcaggcggg gatgaacatt cagtgcgtgc
      481 cgcaccgtct cacgaatacg ttgctgccag aaggggttgt atttgtaggt catggttaaa
      541 tctccgtatg gttcatacgg aatagccacg tcgtaaaaaa tgcgcagagt ccctgaagtg
      601 acctccggca ggacactgtc tcaggcactc tgtgataacc cgaacggtcg tctccggact
      661 gacggttgaa ttttcagcgt cgttttttaa tgtaaaaaac cggttacggg taaggctgtg
      721 aggagggagt gatggtgata cgcaaaaaga agtgcaggga ctgtggaaac gcgattactc
      781 ataatacagt gtgttgcccg tattgtggtt ctgtcgaccc cttcggctac taccggaata
      841 cagaccgtat cgttaccata ctgctggcgc tgattattgt ggtcctgctg actactgtca
      901 gcgtcagtgt gtgtatactg tgtagctggt gagataacgg ttcatgtatc ccctgagctg
      961 acagtgtacc gtgtatatta agcgtaaccg acaggttctc cttcatacca tattaaattt
     1021 tccaggtatt gccgctgcct tatttcgtcc catacaggaa aactcatctg ggtttctgac
     1081 aaatacgcga aactcacagc ccctcaatat caaccacacg ttgtaagccg gtaatacctg
     1141 aaaatcagcc cattactgcc cacgcctccg gcaccattcc cttttgattt cgaattcttt
     1201 gttgtcgcta taccgcttaa tgttcccggt cctccggtgc agataacacc ggagtcatcg
     1261 tagcgctgct tcaatattca ggtgacatat ttccagacgg aagccggcgc gcgatcgtaa
     1321 agtttgttca ttgtcagttc tgccagccgg ttgtcaacgg cagagttaaa ggcttccagc
     1381 tccgcaaggg taagtttata acgcgtgtgg gaattcactt ttcccgcgtc tcctgacgta
     1441 ttacgggctg ccacttacct ggtgtcccgc aatacagcct tagtgttccg ttatcgggga
     1501 tccggacgca ccgcaacaac cggggaagta tttctggttt agagcagtgt caagagggat
     1561 ttctcagccc ctcactcacc ggctcagtct cagggcagga gaattaatac gctcagttca
     1621 tgtcttatac aggaatgggc acagggggag ctggcgtgtc actgctgatt ttacgcatca
     1681 gcgttcatgt atctgcactc tgatacatga acgattcaca gcgctctgtt accggagaga
     1741 tttactctct tagcttactc tggcaaatcc tttacgttac tctctgatga cttcttatat
     1801 ttataatacc aatcagacag ccttatccca tcggtgatat cagtctcctc ctcgccagga
     1861 acgggctgac tcactgtaac cacctccgaa aacaactctg caattatcag aacgcctgct
     1921 tctctccctg tcctcacgaa aactatcccc tctttatcgc gcgtgcgtgc ggaagcatct
     1981 tttcgcaaca accacccggt ataccggtac ggctcagcca ccgcaacctc cccgtttatc
     2041 tcctggcggt cacattcccg attatttttt acgtttctcc ccggttgtta tgccggtgaa
     2101 ggtggcgcgc tgttttcatg accacaccgg ctggtttaac aacatccgga ggaacattct
     2161 catggctacc ccagtttcac tgatggatga ccagatggtc gacatggcat ttatcactca
     2221 actgaacggc ctgacagata agtggtttta caggctcatc agggatgggg cctttccggc
     2281 tcccatcaag ctgggccgca gctcccgctg gctgaaaagt gaagtggaag cctggctaca
     2341 ggcgcgtatt gcacagcccc gcccgtaatt tctgaccgtt atccgttcac ccgcagcaga
     2401 cgtaatcccc ggcctgccgg cggcattctg ctgcctgtta tatccccgtg aggaatatga
     2461 aaatgaaaca acagtatcag acccgctacg aatggctcca cgaaagctac cagaaatggc
     2521 tgaccggctt cacccggcac gccgtatcct ggggcgtgtg tcatccgaat atctactact
     2581 tccataatct gacgcccggt tgggtgtcat tcaatggtga aaaaccggag attgccatcg
     2641 taccccagag cctgcaccgg ctgatttatg gccctgacaa acgggccacg ccgcccctgg
     2701 atgatgatct gattgtgaat ttatgcacca gtgaacatct gctggttcat catccgatgc
     2761 tggaaggcat tctcctgtct gaatgtgaac gtctgaggca gcgttctctg gcaaataaac
     2821 tgatcagcct cttccgtcag tttggtggca cggagttgcg cctcaaactg gtctggcttt
     2881 gctggcttga tttaatgacc ggaaacagcc ttgaagactg gaaagagaac ctgaaacgga
     2941 aatcagaaaa agagctggag gaatggatca tcaaccgtca gaggcagagc gcagcactga
     3001 cggatctgat ggatcagtac gtgctcctgg cataccgcac aacggttgac gatagccgca
     3061 actgatgccg gttgcttccg gaaggtgtga caaaatacgg aaatgatgat gctcccggcc
     3121 ccgcatgctc acctgcagag cgtgttcgtc gtcatcatac tgagccatga cgggcgtctt
     3181 aagggcatcc tgtcattgct gagcaggcaa caccaccaga ggagccacgg acagggatgt
     3241 tctttacagc ccgatacaca ccaggttctc agcagactga atgtgcaggt gcggttattc
     3301 accagaaatg gcatgctgcg gtacataccc atcctggtga caatatgtac gtggcgacag
     3361 cgatcccggc actgtggaag agactcactc agcctgcagg atattctttc cggcatccgg
     3421 gaatgtccgc ctgaatgcaa acaggatgat atttgaatcc gtaatactgt tctttcatca
     3481 taaataattt atgcagatac agttaaccgt tacagtagcc ggtttgtttt accgggaaat
     3541 aatatccgtc agactgatgc cggaacatat ctgaaacacg ttatgatgtt ttgttatcag
     3601 aaatgttgta tattcttatc cgtggcgaca gtttaattgg atttgcccct atatttacgg
     3661 gacacgcctc ttttcattgt gctccttttc agacttcatt ttccgtaagt gttccccggc
     3721 ataaaggagg tacctttctg gcgtttataa ctctctttat cggtgacgcc ggtaatacag
     3781 ggaataccac accgacaaac gaggtattaa cagacgatat ttcagtgtcc ataatttttc
     3841 tgtccggtta aataacgtca gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc
     3901 ataccgggtt acaacgtaaa aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac
     3961 atgtttacct ttcaataccc cctgtctgca gtctccactc ttccatcctc ttaagctacg
     4021 tcggccacat gagtgcactt ccatgataac aggtattcct gtcagcataa cgaagccggt
     4081 taccttccct tcctcctgca tttcctgtca gaatgactgt acagcgcgca gattgccttt
     4141 ttattgacca cctt
//
LOCUS       DADTFG010000237         4051 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.239, whole genome shotgun sequence.
ACCESSION   DADTFG010000237 DADTFG010000000
VERSION     DADTFG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.239"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..421
                     /locus_tag="J1138_004492"
     CDS             <1..421
                     /locus_tag="J1138_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HBA8456178.1"
                     /translation="DEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYWTEYAIQHNQIK
                     FLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWILDFCCKPGFGR
                     KVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            607..1848
                     /locus_tag="J1138_004493"
     CDS             607..1848
                     /locus_tag="J1138_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA8456179.1"
                     /translation="MKWQGRAILLPGIPLWLIMLGSIVFITAFLMFIIVGTYSRRVNV
                     SGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKSTRSGIVTDNHRR
                     DIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQRAEEGIKIMKNN
                     MENYRNYQTKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQITTLESQIQTQAA
                     DFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVGQMVNTGDSLLQ
                     VIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSATVKTISRTPAST
                     QEMLTYKGAPQNTPGASVPWYKVIAMPEKQIIRYGEKYLPLENGMKAESTLFLEKRRI
                     YQWMLSPFYDMKHSATGPLND"
     gene            1823..3937
                     /gene="mchF"
                     /locus_tag="J1138_004494"
     CDS             1823..3937
                     /gene="mchF"
                     /locus_tag="J1138_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export transporter
                     peptidase/ATP-binding subunit MchF"
                     /protein_id="HBA8456180.1"
                     /translation="MQQDRSMTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLA
                     MICGHFGKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELNELGALKMP
                     CILHWDFSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFQAE
                     TQQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGL
                     LTLISAGLMFFILLKAAVSMLRAWSSLVMSTLINVQWQSGLFNHLLRLPLAFFERRKL
                     GDIQSRFGSLDTLRATFTTSVVGAIMDSIMVVGVFVMMMLYGGYLTWIVLGFTMAYVL
                     IRLVTYGYYRQISEETLVKGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAI
                     NSGIKLIRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSD
                     RVASLTSFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYD
                     SQSAQVFSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQL
                     GINNYHRMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECARASHIHDVIMKMPMG
                     YETLIGELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMN
                     ITRVIIAHRETTLRTVDRIISI"
BASE COUNT         1147 a          755 c         1017 g         1132 t
ORIGIN      
        1 agatgaatgg actgtttatg aacagatcgg ttttgcggtc agttgtatgc tctacaatcg
       61 taattacagt ctgtatccgg tgttaaccat tcaatactgg actgaatatg cgatacagca
      121 taatcagatt aaattcctgt ttgattcacg aggttttcca ctggcgtata ttacttgggc
      181 atatcttgag gctgatacag aagcgcgcct gctcagggat ccagaattca ggttgcatcc
      241 gtctgaatgg aatgaagatg gaaggatctg gattctggat ttctgttgta aaccaggctt
      301 tggtcgaaaa gttattgact atctcataca gcttcagcca tggggagaag gagaagtacg
      361 atggttaagc aggcgaaaga aaattgtgac atacatccct gagcggctgc ataaaacgta
      421 gtacctctga agatacagag ataattgtaa attacggggt aaatgcatcg ctgatactat
      481 tttgacagga ctctgtattt tctggctttg attatagcat ttcggttaac cagtgaaggt
      541 attacaggaa aagtgtgagt aagaggagct attttgtttc ttcaggatgc tttagaaaac
      601 agaaaaatga agtggcaggg acgggcaata ttacttcccg gaataccact atggttaatc
      661 atgctgggaa gcatagtgtt tattacggca tttctgatgt tcattattgt tggtacctat
      721 agccgccgtg ttaatgtcag tggtgaggtc acaacctggc caagagctgt caatatatat
      781 tcaggtgtac agggatttgt tgtcaggcag tttgttcatg aaggacagtt gataaaaaaa
      841 ggggatcctg tttatctgat tgacatcagt aaaagtacac gtagtggtat tgtcactgat
      901 aatcatcgtc gggatataga aaaccagctg gttcgtgtgg acaacattat ttcccgtctg
      961 gaagaaagta aaaaaataac gctagatacc ctggaaaaac aacgcctgca atacacagat
     1021 gcgtttcgtc gctcatcaga tattatacag cgtgcagagg aagggataaa aataatgaaa
     1081 aacaatatgg agaattacag aaactatcag acaaaagggc tgattaataa agatcagtta
     1141 actaaccagg tggcattata ttatcagcaa caaaacaatc ttcttagcct gagcggacag
     1201 aacgaacaga atgccctgca gataaccact ctggagagtc agattcaaac tcaggcagca
     1261 gattttgata accgtatcta ccagatggaa ctgcaacggt acgagttaca gaaagaactg
     1321 gttaacactg atgtggaggg cgaaatcatt atccgggcgt tgactgacgg gaaagttgac
     1381 tccctgagtg tcactgtcgg gcaaatggtc aataccggag acagccttct gcaggttatt
     1441 cctgagaaca ttgaaaatta ttatcttatt ctctgggtcc cgaatgatgc tgttccttat
     1501 atttcggctg gtgacaaagt gaatattcgt tatgaagcct ttccggcaga aaaatttggg
     1561 cagttctctg ctacggttaa aactatatct aggactcctg cgtcaacaca ggaaatgttg
     1621 acctataagg gagcaccaca gaatacgccg ggcgcctctg ttccctggta taaagtcatt
     1681 gcgatgcctg aaaagcagat tatcagatat ggcgaaaaat acctccctct ggaaaatgga
     1741 atgaaagccg aaagtacact atttctggaa aaaaggcgta tttaccagtg gatgctttct
     1801 cctttctatg acatgaaaca cagtgcaaca ggaccgctca atgactaacg ggaatttcag
     1861 acaaattata aatcagcttg atatgcgctg gcgacgtcgt gttccagtta ttcatcagac
     1921 ggagaccgct gaatgtggac tggcctgcct ggcaatgata tgcggtcatt ttggtaagaa
     1981 tattgacctg atatctcttc gccggaagtt taatctctcg gcccgtggag caaaccttgc
     2041 aggaatcaat ggaatagcgg agcagctggg gatggtcacc cgggctcttt cactggagct
     2101 gaatgagctt ggtgccctca aaatgccgtg tattctccac tgggatttca gtcacttcgt
     2161 cgtgctggtc agcgtaaagc gtaaccgtta tgtactgcat gatccggcca gaggcagaag
     2221 atatctcggt cgggaggaaa tgagccggta ttttacgggc attgcactgg aggtctggcc
     2281 cggaagtgaa ttccaggcgg aaacccagca gacccgcata agtctccgtt cactgattaa
     2341 cagtatttac ggtattaaaa gaacactggc gaaaattttc tgtctgtcag ttgtaattga
     2401 agcaatcaat ctggtaatgc cggtggggac tcagctggtt atggatcatg cgattccggc
     2461 gggggacaga gggctgctga cgcttatttc tgctggcctt atgttcttta tattgctcaa
     2521 ggccgcggtg agtatgctgc gtgcatggtc ctcactagtt atgagcacgc tcatcaatgt
     2581 acagtggcag tcgggtctgt ttaaccatct tctcagactg ccgctggcgt tttttgaacg
     2641 ccgtaaatta ggtgatatcc agtcgcgttt tggctccctt gacactttga gggccacctt
     2701 taccaccagt gtggttgggg caatcatgga cagtattatg gttgtgggtg tttttgtgat
     2761 gatgatgtta tatggaggat atcttacctg gatagttctc ggttttacca tggcttacgt
     2821 tcttattcgt ctggtgacat acggctatta ccggcaaata tcggaagaaa ctcttgtcaa
     2881 gggggcccgg gccagctcct attttatgga aagcctgtat ggtattgcca cagtaaaaat
     2941 ccagggtatg gccgggatcc ggggaacaca ctggcttaac ctgaaaatag atgcgatcaa
     3001 ttcaggtatt aagttaatca ggatggattt gctcttcggg ggaataaata cttttgttgc
     3061 cgcctgtgat caggtggcga ttttatggct gggtgcaagc cttgtgatcg ataatcagat
     3121 gacaataggg atgtttgtgg catttggttc ttttcgtggg cagttttcgg atcgggttgc
     3181 ttcgctgacc agttttcttc ttcaactgag aataatgagt ctgcataatg agcgcattgc
     3241 agatattgca ctacatgaaa aggaagaaaa gaaaccggaa attgaaatcg ttgctgacat
     3301 gagccctgtt tcactggaaa ccactgattt aagctaccgg tatgacagcc agtcagcaca
     3361 ggtattcagt ggtctaaatt tgtctgtggc tccgggagaa agtgtggcta taactggtgc
     3421 ctccggtgcc ggaaaaacca cattaatgaa agtattatgt ggactgtttg aaccagatag
     3481 tggaaaagta ttggttaatg gcacggatat acgtcaactt ggaataaata attatcaccg
     3541 tatgatagcc tgtgttatgc aggacgaccg gctattttca ggctcaattc gtgaaaatat
     3601 ctgtgggttt gcagaagaaa cagacgacga atggatgaca gaatgtgcca gagcaagtca
     3661 tattcatgat gtgataatga aaatgccaat ggggtatgaa acgttaatag gtgaactggg
     3721 agagggactt tctggtggtc aaaaacagcg tatatttatt gcacgagcct tataccggaa
     3781 accaggaata ttatttatgg atgaggctac aagttctctt gatacagaaa gcgaacgttt
     3841 cgtgaatgct gctataaaaa agatgaatat cacccgggtg attattgcac acagagaaac
     3901 tacgttgaga actgttgaca ggattatttc tatttaaaat ccactggtgt aactgtgtaa
     3961 ggagttttgt cgatggggga ggttaagaag gatgtaaaaa taacagtaat tgcttttgtt
     4021 atcaattatc tgttctttta tatcccggtg t
//
LOCUS       DADTFG010000238         4047 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.240, whole genome shotgun sequence.
ACCESSION   DADTFG010000238 DADTFG010000000
VERSION     DADTFG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.240"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            107..310
                     /locus_tag="J1138_004495"
     CDS             107..310
                     /locus_tag="J1138_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBA8456181.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            318..1250
                     /gene="aaeA"
                     /locus_tag="J1138_004496"
     CDS             318..1250
                     /gene="aaeA"
                     /locus_tag="J1138_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBA8456182.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1256..3223
                     /gene="aaeB"
                     /locus_tag="J1138_004497"
     CDS             1256..3223
                     /gene="aaeB"
                     /locus_tag="J1138_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBA8456183.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELKIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            3315..3587
                     /locus_tag="J1138_004498"
     CDS             3315..3587
                     /locus_tag="J1138_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456184.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3642..3905)
                     /gene="yhcN"
                     /locus_tag="J1138_004499"
     CDS             complement(3642..3905)
                     /gene="yhcN"
                     /locus_tag="J1138_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBA8456185.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          949 a          970 c         1065 g         1063 t
ORIGIN      
        1 atcaatctac agcaatgttg ctctcgcccg gctttccatg cccttctctg cggcgacaga
       61 tgctgaaaat aataacgcct gctctctcta tacaaccaag gtcaacatga gtctgtttcc
      121 cgttatcgtg gtgtttgggc tgtccttccc accgatattt ttcgaattgc ttttatcact
      181 ggcgattttc tggctggtgc gccgggtact tgtgccaaca ggtatttacg actttgtctg
      241 gcatccggcg ttgttcaaca ccgcgctcta ttgctgcttg ttttatttga tatcgcgact
      301 gttcgtttga ggttgaagtg aaaacactaa taagaaaatt ctcccgtacg gccatcacgg
      361 tcgtattagt cattctggcc ttcatcgcaa tttttaatgc ctgggtctat tacaccgaat
      421 ccccgtggac gcgtgacgcg cgctttagcg ctgacgtcgt tgcgatcgcg ccggacgttt
      481 ctggactcat tacccaggtg aatgttcatg ataaccagct ggtgaaaaaa ggacaggtac
      541 tgttcaccat cgaccagccg cgttatcaaa aggcgcttga ggaagcgcaa gccgatgttg
      601 cttattatca ggtactggcg caggagaaac gccaggaggc cggacgtcgt aaccgtctcg
      661 gtgtgcaggc gatgtctcgc gaagagatcg accaggccaa caacgtacta caaacagttc
      721 tgcatcagtt agcgaaagcg caggcgaccc gcgatctggc aaaactggat cttgaacgta
      781 cagtcatccg cgcgccagca gatggctggg tgaccaacct caacgtctat accggtgagt
      841 ttattactcg aggatcaacg gcggttgcgc tggtgaaaca gaactccttc tatgtactgg
      901 cctatatgga agaaactaag ctggaagggg tgcgccctgg gtatcgtgcg gagatcacgc
      961 cgcttggcag taacaaagtg ctgaaaggga ctgttgatag tgttgccgca ggggtcacca
     1021 atgccagcag cacgcgtgac gacaaaggga tggcgaccat cgactctaac cttgaatggg
     1081 tacgtttggc gcaacgtgtt ccggtacgta ttcgtctcga caaccagcaa gagaacatct
     1141 ggcctgcggg caccactgct acggtggtag tcactggcaa acaagatcgc gacgaaagtc
     1201 aggattcgtt cttccgtaaa atggctcatc gcctgcgtga gtttggttaa tcacgatggg
     1261 tattttctcc attgctaatc aacatattcg ctttgcggta aaactggcga ccgccattgt
     1321 actggcgctg tttgttggct ttcacttcca gctggaaacg ccacgctggg cggtactgac
     1381 agcggcgatt gttgccgccg gtccggcctt tgctgcggga ggtgaaccgt attctggcgc
     1441 tattcgctat cgtggctttt tgcgcatcat cggcacattt attggctgta ttgccggact
     1501 ggtaatcatc attgcgatga tccgagcacc attattgatg attctggtgt gctgtatctg
     1561 ggccggtttt tgtacctgga tatcctcgct ggtacgaata gaaaactcgt atgcgtgggg
     1621 gctggccggt tataccgcgc tgatcattgt gatcaccatt cagccggaac cattgcttac
     1681 gccgcagttt gccgtcgaac gttgtagcga gatcgttatc ggtattgtgt gtgcaattat
     1741 ggcggatttg ctcttttctc cgcgatcgat caaacaagaa gtggatcgag agctggaaag
     1801 tttgctggtc gcgcaatatc aattaatgca actctgtatc aagcatggcg atggtgaagt
     1861 tgtcgataaa gcctggggcg atctggtgcg acgtactacg gcgttacaag gtatgcgcag
     1921 caacctgaat atggaatctt cccgctgggc gcgggccaat cgacgtttaa aagcgatcaa
     1981 tacgctatcg ctgacgctga ttacccaatc ctgcgaaact tatcttattc agaatacgcg
     2041 cccggaattg atcactgata cttttcgcga attttttgac acaccggtag aaactgcgca
     2101 ggacgtccac aagcagctca aacgcctgcg gagagttatc gcctggaccg gggaacggga
     2161 aacgcctgtc accatttata gctgggtcgc ggcggcaacg cgttatcagc ttctcaagcg
     2221 cggcgttatc agtaacacaa aaatcaacgc caccgaagaa gagatcctgc aaggcgaacc
     2281 ggaagtaaaa gtagagtcag ccgaacgtca tcatgcaatg gttaacttct ggcgaaccac
     2341 actttcttgc attctgggca cgcttttctg gctgtggacg ggctggactt ccggcagtgg
     2401 tgcaatggtg atgattgcgg tagtgacgtc actggcaatg cgtttgccga atccacgcat
     2461 ggtggcgatc gactttatct acgggacgct ggcagcgcta ccgttagggc tgctctactt
     2521 tttggtgatt atccctaata cccaacagag catgttgctg ctgtgtatta gcctggcagt
     2581 gctgggattc ttcctcggta tagaagtaca gaaacggaga ttgggctcga tgggggcgtt
     2641 ggccagcacc ataaacatta tcgtgctgga taacccgatg actttccatt tcagtcagtt
     2701 tctcgacagc gcattagggc aaatcgtcgg ctgtgtgctc gcgttcaccg ttattttgct
     2761 ggtgcgggat aaatcgcgcg acaggactgg acgtgtactg cttaatcagt ttgtttctgc
     2821 cgctgtttcc gcgatgacta ccaatgtggc acgtcgtaaa gagaatcacc tcccggcact
     2881 ttatcagcag ctgtttttgc tgatgaataa gttcccaggg gatttgccga aatttcgcct
     2941 ggcgctgacg atgattatcg cgcaccagcg cctgcgtgat gcgccgatcc cggttaacga
     3001 ggatttatcg gcgtttcacc gacaaatgcg ccgcacagca gaccatgtga tatctgcccg
     3061 tagcgatgat aaacgtcgtc ggtactttgg ccagttgctg gaagaactga aaatctacca
     3121 ggaaaagcta cgcatctggc aagcgccacc gcaggtgacg gaaccagtac atcggcttac
     3181 gggtatgctc cataagtatc aacatgcgtt gaccgatagt taagtcaaaa ccgacgcctg
     3241 aagcgtcggt tttttcatgg ctatacttag cgatgaacgg cagaactcgc cgcgaaacgt
     3301 gacggtggca acagatgaat atttatacct ttgattttga tgagattgag agtcaggagg
     3361 atttttatcg tgactttagc caaacctttg gtctggcgaa agataaggta cgcgatctcg
     3421 actcactatg ggatgtgtta atgaacgatg tcctgccgct accacttgag attgaatttg
     3481 ttcatctggg agagaaaacg cgtcgccgtt ttggcgcgtt aatattgctg tttgatgagg
     3541 cagaggaaga gctggaaggg catttgcgtt ttaatgttcg tcattagcgc aaaaaaagcc
     3601 cccgaaccgg gggcaatatc gtcggacaag acgatgaggg tttatttgta cagttcagcc
     3661 gtagcgtgcc aggtgtcacc actacgagct tcagtaatct ggtaggccgt tgcgcctttc
     3721 tcttccgctt ttttgttcag catttcacgc atatccattg gcgaagacgc cacaccactt
     3781 acggatacgg tcccgattgc ttcacgattt tgtgcttgtg cagcatcaat ggagtcggcg
     3841 gcgaatgcac cgaaagagag aacagaaagt acgcttaatg cagcaacagt ggttttgatt
     3901 ttcatgattt ttacctcgac ataatctttt agctgggcct ttgtttcgtg acccatatca
     3961 caaaatcaag tatacactaa ttattgaact aattaatacc acgctaactg tttcagttga
     4021 tactaagtgt taaaaatgtg aatttta
//
LOCUS       DADTFG010000239         3986 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.241, whole genome shotgun sequence.
ACCESSION   DADTFG010000239 DADTFG010000000
VERSION     DADTFG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.241"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            80..829
                     /locus_tag="J1138_004500"
     CDS             80..829
                     /locus_tag="J1138_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456186.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            962..2431
                     /locus_tag="J1138_004501"
     CDS             962..2431
                     /locus_tag="J1138_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA8456187.1"
                     /translation="MKANFTLSDGDKAVTDADGKAKVTLKGTKAGAHTVTASMVGGKS
                     EQLVVNFTADTLTAQVNLNVTEDNFIANNIGMTRLQATVTDGNGNPVEGIKVNFRGTS
                     VTLSSTSVETDDQVFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKVDVNSATIT
                     SQEIPEGQVMVAQDIAVKAHVNDQFGNPVTHQPATFSAAPSSQMIISQNTVSTNTQGV
                     AEVTITPERNGSYTVKASLANGASLEKQLEAIDEKLTLTSSPLIGVNAPKGATLTATL
                     TSANGTPVEGQVINFSVTLEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNG
                     VTIQTQTTVKVTGNPSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVY
                     FALKSGSTTLTSLTAVIDQNGIATTSVKGAITGSVTVSTVTSAGGMQTVDISLVAGPA
                     DASQSILKNNQSSLKGDFTDSAELHLVLHDISGNPIKVS"
     gene            2444..2920
                     /locus_tag="J1138_004502"
     CDS             2444..2920
                     /locus_tag="J1138_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HBA8456188.1"
                     /translation="MQSGTNVPYMKISAIDYSQNINGDYKATVTGGGEGIATLIPVLN
                     GVHQAGLSTTIEFISAETRPMTGTVSVNSANLPTASFPSQGFTGAYYQLNNDNFAPGK
                     TAADYSFSSSASWVGVDATGKVTFKNDGDSNTVIITAPPRSGGAIYQTVPPESRSV"
     gene            complement(3048..>3986)
                     /locus_tag="J1138_004503"
     CDS             complement(3048..>3986)
                     /locus_tag="J1138_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBA8456189.1"
                     /translation="GDAMAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLT
                     LVTMTMQDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQV
                     VGLTMKCYSPLCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEV
                     REEMRSLGSYIALNLDGSSQERTFSLSIAENLIAKIQSETDMPIVIVYGPKGEDKARA
                     LVDCYNNVYRLSLSPSIKRSAAIIKDAYMAITPDTSILHMASAYNTPVVAIYADYKTR
                     WPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1172 a          911 c          895 g         1008 t
ORIGIN      
        1 gattaatcat aaataaatac ttgtgacacc aatctttttc cttaacggaa cgaattgttg
       61 tgtagaagga gataatatta tgtcaaaaaa atatcagcca ttgcttataa ctcattatat
      121 gtcaacatgg gtcactataa cggaagcagt tgagatcact accaaagcca taaaacaaaa
      181 aattactcct agcgatattt atcgtcatgc cttgagtggc aacatcctac tatcggtcta
      241 tttccagtct cctgtgatac ttaagaagat acaaactttt aatggaaaaa taaaattcag
      301 gcagtttgtg ggagacctac ttgataaact atgcatgctt gacagggacg gttttattta
      361 tggtcaaaat ttaagactct gtacagaagc cagatatatt tgtcctgtgc aacaaattat
      421 agataccccc ctgttacgca aactcaacca gtttcgtacg tttgttcgta atgtccgccc
      481 gggtgatgaa ctggatgtcc aggcacaagt tagtgaaaaa aatttaaccc cgccgccggg
      541 taacagcagt ggcaaccttg agcaacagat agccagtact tcacagctaa ttggatctct
      601 gctcgccgaa gatatgaaca gcgagcaagc ggcaaacata gcacgtggat gggcctcttc
      661 tcaggcttca ggggtaatga cagactggtt aagccgcttc ggtaccgcaa gaatcacgct
      721 gggcgtggat gaagatttca gcctgaagaa ctcccgtgac ggcaaccctc gaaaatggcg
      781 attcgatgca acaaacagtg aactatgtgc cgaacgtcac gaatgctgaa atcacgctgg
      841 cagcctcgaa ggatccggtg attgccgaca ataacgatct cacgacacta acagcaccgt
      901 cgctgataca gagggcaatg cgatagccaa cactgaggta acatttactc taccggaaga
      961 tgtgaaggcg aacttcacgc tgagcgatgg cgacaaagcg gttactgatg ccgatggcaa
     1021 agcgaaagtc acgctgaaag gtacaaaagc aggcgctcat accgtgacag catcgatggt
     1081 tggcggtaag agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt
     1141 taatcttaac gttaccgagg acaatttcat cgccaataac attggaatga ccaggctaca
     1201 ggcaacagtg actgatggaa acggcaaccc agtagaaggt ataaaagtta atttccgcgg
     1261 aacctccgtc acgctaagca gcaccagcgt tgaaacggat gatcaggttt tcgctgaaat
     1321 tcttgtgaca agcaccgagg tcggactaaa aacagtttca gcctctctgg cagataaacc
     1381 tactgaagtc atctcgcgat tactgaatgc caaggtagat gttaattctg cgacgattac
     1441 cagtcaggag atacctgaag gtcaggtcat ggtcgcacaa gacatagcag ttaaagctca
     1501 cgttaacgac cagtttggca acccggtgac gcatcaaccc gcgacattca gtgcagcgcc
     1561 atcctcgcaa atgatcatca gccagaatac ggtctctact aatacgcagg gagtagccga
     1621 ggtcaccata acgcctgaaa gaaacggctc gtatactgta aaagcatccc tagcgaatgg
     1681 agcttcactt gagaaacaac tggaggctat tgatgaaaaa ctaacactca cgtccagtcc
     1741 gcttatcggt gtcaatgctc ctaaaggcgc tactctgacg gcaacgctaa cctctgcaaa
     1801 tggcactcca gtggagggtc aggtcatcaa ctttagcgta acgctagaag gtgcgacgtt
     1861 aagtggcgga aaagtgagaa ccaactcttc aggtcaggct ccagtcgttc tgaccagcaa
     1921 taaagtcggt acatatacgg tgactgcatc gttccataac ggcgtcacaa tacagacaca
     1981 gacaaccgtg aaggttactg gcaacccaag caccgcacat gttgctagct ttatcgctga
     2041 tccgtcgact atcgccgcca ccaacagtga tttaagtacc ttaaaggcaa cggttgagga
     2101 tggcagtggt aacctgatcg aaggtctcac tgtgtacttc gccttaaaaa gcggctccac
     2161 cacattaacg tcattaacag cggtgatcga tcaaaacgga atcgcgacaa caagcgtgaa
     2221 aggagcgata acggggagcg tcactgtaag cacagtcaca agcgcaggtg gaatgcaaac
     2281 agtagatata tcgctagtgg caggcccagc agacgcctcg cagtccatcc ttaagaacaa
     2341 ccagtcatca ctgaaagggg actttaccga tagtgctgag ctacatcttg ttctgcacga
     2401 tatatcaggc aatccgatca aagtttctta agggatagaa tttgtgcaat caggtacaaa
     2461 cgtgccctat atgaaaatta gcgcaattga ttacagtcaa aatatcaacg gtgattacaa
     2521 agctactgtt acaggaggcg gagagggtat cgcaacgctg atccccgtat tgaatggtgt
     2581 tcatcaggcg ggtctgagta ccacgataga attcattagc gcggaaacca gaccaatgac
     2641 gggtacagtg tcagtgaata gtgcaaacct accgacagct tcattccctt cgcagggatt
     2701 taccggggcg tattatcagt tgaataatga caactttgca ccaggaaaaa cggcggctga
     2761 ttattcgttt tcaagctcgg cctcctgggt aggcgttgat gctactggta aagtgacatt
     2821 caaaaatgac ggtgatagca atactgtaat aatcacagcg cccccaagat ccggtggtgc
     2881 tatttaccaa acagttcctc ctgaatcgcg ttcggtgtga ctatgatcat tttcggtgag
     2941 tattgctttg atccaaaggc tggttttctg tgatgattga ccagcctcag agtattgatg
     3001 acaccaccgt tcataacaga tcggcattac tatgcataag tactgcctca aattctcgcc
     3061 aaaacacttt ttaatgcctt tgcgaattca tccagactta tattgtcaat tttttgccca
     3121 acgacgactg actccgaaac atcggccatt gcgggccatc gcgttttgta atcagcataa
     3181 attgcaacaa caggggtatt ataggcactt gccatatgta atattgaggt gtcaggagtt
     3241 attgccatat aagcatcttt tatgattgct gctgaacgtt taatcgaggg tgataaggat
     3301 aaacggtaga cattattata acaatccact aatgccctgg ctttatcttc ccctttgggt
     3361 ccataaacga tcactattgg catatctgtt tcactttgaa tttttgctat tagattttct
     3421 gcaatcgata atgaaaatgt acgttcctgc gagctaccgt ctaaattgag cgcaatgtaa
     3481 gatcctaacg atcgcatttc ctcgcgtacc tcatcgagta catcatcgct taatggcaat
     3541 tcatatattg acctgactgc cggaaaaccc gcctcacgca tcataaatgc ccatgtcatg
     3601 ggtacaggag cccggagatt ctgatccatc cgggacgcgt tcttacacaa aggggaatag
     3661 cacttcatgg ttaaaccgac gacctggaaa ttcgtttttg ctttcaactg actgataaat
     3721 atcatcgttt tcaggttctt tttacgcatc gcctctatac aaagatccgg cgtaccgtat
     3781 tgcagtgtta tgttcttcgc taaatctttt atttctttta atgttgcgtg atcctgcata
     3841 gtcattgtga ctaatgttaa tttagtctgt tcaagtttga gcgcattaaa gacttctaaa
     3901 ttaattgtcg atgtaacaat taatagatgc ttaattttat gcaattcaag cgcccgaata
     3961 acaggaaaga tggccatagc atcgcc
//
LOCUS       DADTFG010000240         3977 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.242, whole genome shotgun sequence.
ACCESSION   DADTFG010000240 DADTFG010000000
VERSION     DADTFG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.242"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..346)
                     /locus_tag="J1138_004504"
     CDS             complement(<1..346)
                     /locus_tag="J1138_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA8456190.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQML"
     gene            complement(501..659)
                     /locus_tag="J1138_004505"
     CDS             complement(501..659)
                     /locus_tag="J1138_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBA8456191.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(730..3575)
                     /gene="ag43"
                     /locus_tag="J1138_004506"
                     /pseudo
     CDS             complement(730..3575)
                     /gene="ag43"
                     /locus_tag="J1138_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
BASE COUNT          835 a         1156 c         1041 g          945 t
ORIGIN      
        1 gcagcatctg gtagctggag gtaccgtcat gagagttgag cagaataatt tcagggacat
       61 gttctccgtt tatctctccg gcccgccgca gacgcagcat gtgttttgtg tatccccggc
      121 ggcccgggtc gcgcacacgg gtctggcagg cgaagaatgg ctgaaagcct tcccgctgca
      181 ggctttccag tacggtgatg gtgggaatgt acgcatatcg tttactgcgg gaagtatgcc
      241 ggtcttcacc gaaaatactg ggaacatagt gcatcagttc ttcgtgtgtc agcggacggt
      301 cacggcgtat ctggttcgca taaccaaaac gactggctag tcgcataatt tgctccttat
      361 cggtggttac gatttactgg tgtaataaat gaaaaagcca cgtctcccgg agaagacgcg
      421 gcctgacaga tgaaatgaat gacgtttatt gtctgagaag cccttaactg gcgagctgag
      481 tattaagctg tgttccggca tcaccagcgc aactgacctt cagcattacg gataaccagc
      541 cgggaatatg ttccctggtc atcttcagta aacacattgc ggtaagctgt tttgacggca
      601 acagcctgtt cgcgtgagaa agggccttcg ggcaataaac gtgatgtaca acccggcata
      661 tctgttactc cctgaaagta aaaggccccg gtcataatga ccggggcctg aaggggagtg
      721 acctgattat cagaaagtca cgttcagtgt ggcctgaccg ttataacctt cagcgctgct
      781 gccgctgacg ctgtgggcat aaccggcctg aacgcccagg gtgatatttt cccggacacg
      841 ggcttccagt ccggcctgca ggtccagtga cgtgccattc tgtgagggtg agaacgtcat
      901 gttactcccg gctgcggctg ttcccatgct catgtcaccc cgtgagctga aggtgcggat
      961 aacagaaggc tgtacccacc agttcaccgg cagttcacgc acactgtgtt ttgcactgtc
     1021 gcgcagggtg tcacgggatg aggtgccttt accaaagttc atatcgttgt ggctgcccag
     1081 acggaagccg gcacgcacat gttgtgcact gccatgcccg aacttcacat aaccggcgtt
     1141 atcctggccg tcatccaggg agagtccctg ccaggtgtac tgcagttgtg gctccagcat
     1201 cagattgtca gtgatactga agggcagacc ggtttccagt gagcccagcc agccccagcc
     1261 ccgggcgcgg aagtcgttat tgtccgatga cgctttcatg ctgtggcggg ttccctgtgc
     1321 cacaatgtca gcccacaggc cggaggacgt gtgtaccaga ttcaggtatc cgcccagact
     1381 gccggcatca tcccggaccg tgccggcacg ggagccgtca tcatccttaa catcaacgga
     1441 agaatggcct gcagcaccat acactcctgt cgtcagtgac ataccggcaa cctctgttct
     1501 caacaggtca ccctccagac ggacgaggcc atagctgccg ctgctttccg gcgtggcccc
     1561 acgggcaata ccgccgttgt tatcgtgacc gagatgaccg ccctgaatgc tgagacggat
     1621 gctgttattt tcaccgttta caccggtctg atggctgcgg gagcctgcca gaatccggtc
     1681 atagtccatt gcctgtgtca gcatggaggc atacaggggg acttctgcac gataagcatt
     1741 ttcactgcgc aggtaccagt cttcatcgct gtcacggttc agggtgtagt taaaggcgcc
     1801 ggcctgaagc gggcgactca gggcaaacgc accttcttct gtggtggcac cattctgtgc
     1861 atccacaacc cggataccct gtccggaggt tgccaccccg aggttactgt ttccgacatt
     1921 tgtaaacgca agccaggttt tgccggttgc ctgaccacca ttaatcacca gctggtcaga
     1981 ggcattgctg ccatcaaggc gaacacgcat attgatggtg ccaccctgac cggtgaggtt
     2041 actggtggtc agtttatgga acgttaccgg ggcgttgcct tttgcaacag cacggctcag
     2101 tacggcatcc ggtgttgtct gattgtcaaa gtaaatctcc cctgcgttaa caatatcgcc
     2161 ggtactgacc acatcaccat tcagtaccat gctggcgcct ttactgagct gtgttctgcc
     2221 actcagactt cccccggcag ccagcatcag tgttccacga tgtccgacag tggtgttgct
     2281 ggcctgtccc ccggcattaa ccgtaaagct gccgccattt tccagcagca ggccgctggc
     2341 ctgaccggat atgccatcaa tactgaactt accgctggca ttggtcccct caacagtggc
     2401 accactgtct gcaatcaggg caccaccgga tgtcatggtg acacctgttg ccttaccacc
     2461 ggcagacact gccagggttc cgccgtcatc cacccgtgtt ttctgcgctg aatggccttc
     2521 cagtacatcc aggcgaccgc cggactccag aacaacgccg tcagccttac cgttttccac
     2581 cgcgaaattc cccagacggt tgctgccaag aaccgttgct gcagtactgg tgaccagtgc
     2641 gccgccctgc ttcagggtga cattcgtggc tgtaccaccg gcgttcacct gcagtttgcc
     2701 tttctggtta acggtggtga aatccgccag accaccttcc ttgataatct gccagccgtc
     2761 actgttcaca acagtgccag acgccgtacc gccgttgtgc acatactggt accccccatt
     2821 cagcgtggta tccagtgcgt gaccatgtac agtctggtcg ccgccggcat aaaccacagt
     2881 ggtatttgcc gttccttcag cagccacaat ctgacgacca tttttattga tggtggtgcg
     2941 tacggcattt ccgcgaacaa actgcccggt atcaccattt tctgcatccg gccccccttc
     3001 cgcgccggta ttcacaaccg tgtcggttgc cactgcgccg gatttgacgg cctgccagcc
     3061 cttctcatta atgacagtac ccgttgcaat cccgccttca tgtacccact gctcaccgcc
     3121 gttcagagtg gtgtcactgc ctgcccctga aggctctgtc cgcctccggc actgataacc
     3181 gtgtctgaaa cgcttcctcc ggcattcact ctctgaagac caccaccggt gacagtagtg
     3241 ttgttggcga taccgccatt ttgtatccat tgtccgccgg tattggcctc gttatccggc
     3301 ccatactcca gaccggtact gatggtcatt ccgttggccg taccgaagac aatctggttg
     3361 tcatgatttg tcagtgttcc gccgctcacg gtttctcccg cctgtacaac cgtgtcagca
     3421 gccagtgccg ggactgatgt gacagcagca agagacagcg caaccgccac accggcgcgt
     3481 tttccccgtg agcgggccag ttcggaggcc accaccaggg tgcccgtaat gtgattccat
     3541 accagcctgt agctggtgtt cagatgtcgt ttcatcagct tttccttaca gagggtgaat
     3601 aaaaaagccg gtacccacaa aagtggatac cggcaggtac aaaacagcat ggtcagataa
     3661 agtggttttt cccggacata aatgcttccc ctctctcatc cgccagagaa gtatcagtcc
     3721 gttgtgtgca attccgttct gcatgatgaa gagattcagc tgccttttcg caggccatta
     3781 accactataa acgatcgata gtaacgatcg atacctgttt tatcgatcgt tttattctat
     3841 taacggcgag gcggtcacgc aagagaaaga ggtgggtatg aggggtttgt gtcatgtttt
     3901 cagttggtta acacaatgta atcaggcatt tcccctggca aagcgttcag aagaaaatcc
     3961 agttccatac cgcgcgg
//
LOCUS       DADTFG010000241         3969 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.243, whole genome shotgun sequence.
ACCESSION   DADTFG010000241 DADTFG010000000
VERSION     DADTFG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.243"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            149..3918
                     /locus_tag="J1138_004507"
                     /pseudo
     CDS             149..3918
                     /locus_tag="J1138_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1068 a          883 c         1071 g          947 t
ORIGIN      
        1 aataataaga aaatgctttt tgatgatgga aacatcacaa aacacaacta gttaaattgc
       61 tacattcccc gtttgggtga taatgtctga tttttattag catcttcaac ttattaagtt
      121 tatctgtgaa catcatggtg aggtcgtggt gaacaaaatt tattcgttta agtgttgtcc
      181 tgtcaccgta ggtcttattg ctgtctccga acttgccagt agtgtaacga aaaacgcagg
      241 ccgaaaatta aaaaaaatat cgctgatggt tttatctgtg agctgtctct cttatcctat
      301 gatatccttg gcgggcattg tccgttccga tatagcctat cagatttatc gtgatttcgc
      361 tgaaaacaaa gggctttttg tacctggtgc cacagatatt ccggtatatg ataaggacgg
      421 aaatttagtt aaggttgccg gcggatatgc ttttaaaaca ggaggaacca caggagttcc
      481 tctgatatct gatgcaacaa tagtctctaa tcccgggcaa acctatgatc ctgtaaacgg
      541 acctttacct gactatggtg cccctgggga cagtggttct cctttgtttg cttatgatga
      601 acaacaaaaa aaatgggtta ttgttgctgt attaagagca tatgcaggta ttaatggtgc
      661 tacgaactgg tggaatgtca tatcaacaga ttatctgaac caggttatgc aggacgattt
      721 cgatgccccc gtggactttg tttccggact gggccccctg aactggacat acgacaaaac
      781 atcaggcaca ggtaccctga gtcagggcag taaaaactgg accatgcacg gacagaaaga
      841 caatgacctc aatgccggta aaaatctggt attcagcggg cagaacggtg caattgtcct
      901 gaaagacagt gtgactcagg gggccggtta tctcgaattt aaagacagtt acaccgtatc
      961 tgctgaatcc ggaaaaacat ggacggggcc ggcattatta ctgacaaggg gacgaatgtg
     1021 accaggaagg tcaacggtgt tgccggtgac aacctgcata aactggggga aggaaccctg
     1081 accataaacg gaacaggtgt aaaccaggga ggactgaaaa cgggagacgg taccgttgta
     1141 cttaaccagc aggcagacac tgcaggtaat gttcaggcct tcagttctgt gagcctcgcc
     1201 agcggatgtc cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca
     1261 tgggggtacc ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccggctg
     1321 caggctgctg attacggggc ggtgattaca aatcatgcac agcaaaaatt ccgactttta
     1381 ctggatctta aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatacccccc
     1441 tttggtggta ccggcactct aggaaacctg tacagcatga tactcaacgg ccaggcccgc
     1501 ttctatattc tgaaatctgc cagcaatggt aacaccctgt gggggaacag cctgaatgat
     1561 ccggttcagt gggaatttgt tggtacggac aaaaacaaag ctgttcagac agtaaaagac
     1621 tggatcctgg ccgggcgggc aaaacaaccc gttatctttc atggtcagct gtccgggaat
     1681 atggatgtcg ccattccaca gctgccgggg ggaagaaagg tcatctttga tggtagcgtg
     1741 aacctgccag aaggtaccct gagtcaggac agtggcaccc tgacattcca gggtcatccg
     1801 gttatccatg cctccgtccg tggcagtgcg tcggtcagcc tgaaccagaa agactgggaa
     1861 aaccgccagt tcacaatgaa aacactgtcg ctgaaagatg ctgacttcca tctttcacgt
     1921 aatgccacgc ttaacagtga catccagtcg gataacagcc atatcacact gggaagtgac
     1981 agggtatttg tggataaaaa tgacgggacg ggaaattatg tcagtcctga ggaaggtacc
     2041 tctgtcccgg acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat
     2101 aactcaaccc tggatatcgg cagcctgttc accgggggga ttgacgctta tgacagtgcc
     2161 gtcagcatca cctctccgga cgtcctgctt acagccccgg gggcttttgt cggcagttcg
     2221 ctgacagtac atgatggagg tcaccttaca gcactggacg gtattttcag cgacgggcat
     2281 attcaggtcg gtaagaacgg taaaatcccc ctgagcggta caccggttaa agatacggct
     2341 aatcagtatg cccctgctgt atatctgacg gatggatatg agctgaccgg cgataacgca
     2401 gcactggaaa ttacccgtgg agcacatgtt tccggtgata ttcatgcctc tgcggcatca
     2461 acagttacca tcgggtctga caggtcggca gaactggctt ctgcggaaac gcctgcatcg
     2521 gcgtttgccg gtagtcttct tgggggctat aacgcagcat tcaatggtgc tataaccggc
     2581 ggcagggctg atgtcagtat gcataatgca ctgtggactc tgggagtgga ctcctctatc
     2641 catagtctta ctgtcagaaa caaccgtatc cgctctggag gagaccgggc attccgtacc
     2701 ctgaaggtga ataaactgga tgcaacaggc aatgatttta tcctgcgtac ggacctgaaa
     2761 aatgccgata aaatacaggt gactgaaaaa gccactggtt cagataacag cctgaatgcc
     2821 tgctttatga agaatccgtc tcagggacag gccctgaata ttcccttggt cacggtaccg
     2881 gcgggaactt ctgcagaggt gtttaaggcc ggcacccggg ggacaggttt cagtcgggtg
     2941 acaccaaccc tgcatgttga caccagtggt ggcaatacga agtggatact ggacggtttt
     3001 aaagcggaag ctgataaagc cgctgccgcg aaggctgaca gtttcatgat tgccgggtat
     3061 aaaaacttca tgacggaagt caatgatctg aacaaacgta tgggtgacct gcgtgacaca
     3121 aacggtgatg ccggagcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt
     3181 tacagtgata attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt
     3241 gtggacctgt ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca
     3301 ttcagcggca agacgaaatc ggttgggggc ggtctgtatg gttcagcatt gtttgagtcc
     3361 ggtgcctata tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac
     3421 tttgctagcc tgggaacaaa acactacaac acccattcct ggtatgccgg tgctgaaacg
     3481 ggttaccgct atcacttgac agaggacact ttcattgaac cgcaggctga actggtttac
     3541 ggcgcagtat ccgggaaaac attccgctga aaagacggta atatggacct gagcatgaag
     3601 aacagggact tcagcccact gatgggaaga acaggggttg aactgggcaa gaccttcagt
     3661 ggtaaggact ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat
     3721 aatggtgaga ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac
     3781 agtcggatgc tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt
     3841 ctggagtttg agaaatccgc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat
     3901 ttccggtata tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga
     3961 ctattaaaa
//
LOCUS       DADTFG010000242         3949 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.244, whole genome shotgun sequence.
ACCESSION   DADTFG010000242 DADTFG010000000
VERSION     DADTFG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.244"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            160..853
                     /locus_tag="J1138_004508"
                     /pseudo
     CDS             160..853
                     /locus_tag="J1138_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1077..1373
                     /locus_tag="J1138_004509"
     CDS             1077..1373
                     /locus_tag="J1138_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBA8456192.1"
                     /translation="MPDQSGYCWTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1600..1797
                     /locus_tag="J1138_004510"
     CDS             1600..1797
                     /locus_tag="J1138_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBA8456193.1"
                     /translation="MIGKAIIKAQYNKQVPLTLVAMSVLTAMSIACQNQVDVCSPGNL
                     RGAVNIYTMVLEKGKRPGERY"
     gene            2016..3449
                     /locus_tag="J1138_004511"
     CDS             2016..3449
                     /locus_tag="J1138_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBA8456194.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     TSIACQNQVDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKSPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGGIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTMSAMIQPDVYHKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQELFDKRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLLHHLSSEEGDISPYTAEMGRELAIWYGNEYMRLSNPL
                     TFDNTAQNETMRLIPEELELFNWIKSYCIEKGSPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT         1192 a          746 c          872 g         1139 t
ORIGIN      
        1 attatatcat tttgcttttc tgttaactgg ttttcatttt tgaatcaata ttttctgaac
       61 agggtaatag tacacgtatt aacagattat gtcagaaggc cccggtgatg ccttctgacg
      121 atagattaat gtggtattct attggtatct ggagaccata tggcaacagt tacagtacat
      181 tctccccgct gtaattccta taaagtgtac cattatgatc acagcacctc agatcatgag
      241 cgtttccagt gctaatcctg caaacgtgtg tatttcaact gaattacatc tacgaaaccc
      301 gaaaaccagg cattaaagag caaattgttg aaatggttca taacggagtc ggggttcgcg
      361 atagctccag aacgctgaag gtggatatca acaccgtcat tctcacttta taaaacgctc
      421 accacgcaaa gtaaccacat ctccagacgc cagtgccgat gtggcgctta tctgcgaact
      481 tgatgaacaa tggagttttg tcgaaaacaa agctcgtcag cagtggcact ggtatgcgta
      541 taacaccaaa tctggaggtg tgctggctta cacttttggt cctcgcactg atgaaacatg
      601 tcgtgagttg ccggaactcc tgaagccatt ctccacaggc atgataacca gagacaaccg
      661 gagcagttat accagagaaa tgccacagga taagcatctt gtcggcaaga tatttacccg
      721 gcgcattgag cgcaacaatc tgacacttcg tactcacatc aaacgaccag cccggaaaac
      781 gattttcttt tcacgctccc tggaaattca cgaaaagcca ttggttcatt tatcgaaaac
      841 actgtactac tgactggagt cattaccagg gcctcttttg ctgttttttt accaatgaat
      901 gataatgaga atcgacagcg ataaggagaa agtaaaataa caataatgct gttgttttct
      961 atcgtattga tttttaatta tttattgtct ttttgctcag cccatttttt tacgcagaca
     1021 aatttacccg gggaatacaa actgtaacga cagttcacca cactatgggg gataaaatgc
     1081 cagatcaatc cggatactgc tggactcctt cacgcatctt acggatgcct gaactgagtc
     1141 aattgttagg tatatctcgt tcgacaattt acgaaaaaat gaatccgtta tcgaaatact
     1201 acgatgcaac cttccccagg ccggtacgtc ttggtagtgg ttctgtgggc tggcgttcat
     1261 cagctattga cgaatggctt actttacata ctgtacctgc cagatcagca gtcaaaggtg
     1321 ttaatgacga agtgcgacat acgctttctt cagatatgac catgagcaga taagtttttg
     1381 tcatcacgtg atcacctcag gaggaaaaag aagcataaga acaatttagc cagaagttta
     1441 attgataaac agaatttcca gcattcattg ttgaaagagc agctattgtc gctattgtag
     1501 cagtaattgc tgctataacc gaaacgtatt acgaaaaagg aagcatttat gtcttacatc
     1561 aaaccggata tgccgtattc agtatatgcg tgaccatcaa tgatagggaa ggcaataatt
     1621 aaagctcaat acaataaaca agtacctctg actctggttg caatgtcagt attaacagcg
     1681 atgtcaattg cctgtcagaa tcaggttgac gtatgcagcc ccggaaattt acgtggggcc
     1741 gttaatattt acactatggt tctggagaaa gggaaaagac cgggggaaag gtattagtga
     1801 aataatttct tgaggtatta agaatgagaa atatgaatga tatttcgtgg taaataatac
     1861 agtctgcact gaattaacct tatcggttta ttaaacatca ggtttacaaa tgctctgtat
     1921 ttctcagagc atcaggtctg aatagcagct attttcgcta ttgtagcggg agttgctgct
     1981 attttactaa cccattaatg aaaagagagg cttttatgtg cttacttgca ccagaaaatc
     2041 cttatccgat atatgcacta ccaccgctgg tgagaaatgc aataattgaa actcaaaaaa
     2101 atacacaggc acctttggct atggtggcaa catccgcatt aacagcgacc tcaattgcct
     2161 gtcagaatca ggttgacgtg tgcagacctg gaaatttacg tgggcctgtt aatctttact
     2221 ctctgattct ggctgattcc ggtgaaagga aaacgaccgt ggataaagtg tttatgaaag
     2281 cattttatct cagggatgaa gccctggcgg aagaatacgc gaaactggtt gagaattaca
     2341 gtacagaaaa ggaaatatgg gagcaaaaac aaaaagcgct ggaatcaaaa tttcataaag
     2401 agattcgtgc cggtaaagat tacaaggcaa cagaatcaga gcttgaaacg catctgaata
     2461 agtctcctgt tccgccgcag atacgtcgaa caatttttaa tgagacaacg atagagggaa
     2521 tgttaaaata ttactccgat agcaatcgtt cttttgctct tgtatccagt gaaggggggg
     2581 ggatttttga cagccgggcc atgagtaaac tgggaattat taacactttg tgggatggag
     2641 gttctctttt catcgacagg aaatcatctc ccggaattaa tttgaaggag ccaagactga
     2701 cgatgtcggc gatgattcag cctgatgttt accacaaagg tttttgtacg cgaaaaaaag
     2761 aacttgtgaa aacgtcagga catcatgcaa ggtttttgat gtgtcaacca acatcaacgc
     2821 aggggacaag gataataacc ggagataatt attcatcaca gtatcaggag ttatttgata
     2881 agaggatcaa tgaacttatt gatgaaagtc tggctatgag tggtgaacga cgttgccttc
     2941 acttttctcc tcaggctgcc agaatctgga cggattatta caacgatgtg gaatcgaagc
     3001 tggggggatt ggggccttta agacattgtc gggaatatgc tgccaaaaat gcagagtata
     3061 tggcaagact ggcaggactt cttcaccatt tgagcagtga ggagggggac atttcccctt
     3121 acactgcaga aatggggaga gaattagcaa tatggtacgg taatgagtat atgcggttgt
     3181 ctaatccgtt aacttttgac aacactgctc aaaacgagac tatgcgactc attccagagg
     3241 agcttgaact tttcaactgg ataaaaagct attgcattga gaaggggagc ccctgtatga
     3301 aaaaaaatga cattttacag cgtggtccga accgtttccg gaaaaaggat aaaatcaact
     3361 ggttactgga tttactgtat gaacaaaaca gagttgtacc ggttattgag ggaaaaacgt
     3421 tgtgtgtcgc acctaacttt gacctctgaa tgacttaatc gttgagtaac atataatctc
     3481 ctctgctact tgcggaggag actaatgcct ttaccatgtc agcgttgtat tagaaaactc
     3541 tggtacaggt cagtcagtaa atataactgg tgtaaccact atttactatt ggatagagtg
     3601 ttgctcagga tattgtcggc tttagtctga ggtgaggtgg gaccttcatt gttttcgtgt
     3661 tcaataaata atttaggtta attagtatta ttaatgggag ttatgttatc tgttatgttg
     3721 tctctttcta tcgtaatgtc actcctcttt atgctgagta ttctgtatgt gcgtaatgcg
     3781 tctatcccca tatactatag tgtggtggtg tatggggttt agttatgtgt aaattatgtt
     3841 actacactca tagcgtaata ttgagaaagt taatccactg aatgtatgtc tggtttgtat
     3901 ttaacactag ttacgtacca gaacactatt agagtatatt gtctttagt
//
LOCUS       DADTFG010000243         3851 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.245, whole genome shotgun sequence.
ACCESSION   DADTFG010000243 DADTFG010000000
VERSION     DADTFG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.245"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            66..407
                     /locus_tag="J1138_004512"
     CDS             66..407
                     /locus_tag="J1138_004512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456195.1"
                     /translation="MQHNALVGRLITQPGVVVKDDTRFCRFTVAELRKRSGNQTKIEF
                     TAFDAVASHIADKCLVGDTVSINFTITNAVYESDGVNHHGYRFQATLVEFVAAGKQRR
                     EQESQRIGQGR"
     gene            457..723
                     /locus_tag="J1138_004513"
     CDS             457..723
                     /locus_tag="J1138_004513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456196.1"
                     /translation="MSIYMELRCELRGELPINESKCWSEVDRSLWAMALNTHESTEQT
                     TTELLSKATHAGWQSINGDWICPGCQENLALTEQMQAVAERHDQ"
     gene            768..1610
                     /locus_tag="J1138_004514"
     CDS             768..1610
                     /locus_tag="J1138_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBA8456197.1"
                     /translation="MTAKVITICSQKGGIGKTFATTEIAAELQRRSYRTCAVDGDWMS
                     ALTSRQFPDGLPLEIDANPSGRVLTPGTAHVNRIYGEEPFEPLRLHDGRGFMGATEEL
                     NDINNRNSDCIYDFRTRFAELRDQFDYILIDSSPVWSNLLMANHLVADYLLIPTLLEK
                     TSRLGAEKHLRTLKKIKERYNPDLKLLGIVVTQATVANYKKEVLGGRYASVDTENLKM
                     LLEILEENGFGPEHLLEIIPYVQTKVREAIELNYTMKEYAPDSPLVARYDALVDTIIA
                     RTGG"
     gene            1615..2751
                     /locus_tag="J1138_004515"
     CDS             1615..2751
                     /locus_tag="J1138_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBA8456198.1"
                     /translation="MAKNFKAQMREKREKATSPQTQVETPAVERELALPRNDLNQNDL
                     KVNRAEHEDPAGQIIRVPIDDVYALEQVRPEEDFEDEVIDGMKQTYGDFGILMPPRCF
                     PRDKRGYRIWMGETRWRSAKAKGDLFIDIYVGAPPENEKKRIYGQLIENLHQSGLKPL
                     ATAIQLQALRDVHGETQESMSRILGKPKTFISKHMRLIDAPAPVIALLKEKVTKDVEL
                     VYTLCQINDISPDSVDKLAKLVRDGKLTRQGAIKELNVLKGKPPKNPPPPPPPTNSGT
                     ETQEENRNKDVLTEQPGTSTNQTGNTAGEQQNAGNEQTHKTQPLQGESNVNITRVMVK
                     FEGGEGYLLTDRIPEEYGQVWIALPLGELCIDAAEISILGIKEV"
     gene            2752..3189
                     /locus_tag="J1138_004516"
     CDS             2752..3189
                     /locus_tag="J1138_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456199.1"
                     /translation="MSKALFADLAEQFLSVAVKTWVIRSELPAAAAADVLLTTAIQQG
                     FLEDRGRPLLGNTLNEWGKNKKYPRWAIQSAIALLLADGWKPVTHPEWAAYAAIHVQS
                     KKGGSLTQLLDVLPEGLDIDVAAGWICAATEDAARYHERKKLR"
     gene            3186..3506
                     /locus_tag="J1138_004517"
     CDS             3186..3506
                     /locus_tag="J1138_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456200.1"
                     /translation="MTFSDIEAALSSTGRRAYAGVILAFLLDAHDRGAGLVEKHTVVE
                     NVGITDTNVSAVARRLEEQGIIEILYWDTKSESPTWSSLRNGAWSAPGYRLLPAVLQL
                     YRRC"
BASE COUNT         1008 a          934 c         1061 g          848 t
ORIGIN      
        1 ctgtactgat agtggaaagc cactatcgac tctctggcgc aaagccatac tcaaaggaga
       61 atgtcatgca acacaatgca ctcgttggtc gactgatcac tcagcctggc gttgtagtca
      121 aagacgacac acggttttgc cgtttcaccg tagctgaact gagaaaacgc agtggtaacc
      181 aaactaaaat cgaattcacc gcttttgatg ccgtagcctc gcatattgcc gataagtgcc
      241 tggtcggcga taccgtttcc atcaatttca ccatcaccaa tgctgtctat gagtcagatg
      301 gtgttaatca tcatggctat cggtttcagg cgacgttagt tgaattcgtt gccgccggca
      361 aacagcgccg tgagcaagag agtcaacgta ttggacaagg tcgttaactt cccggtttcg
      421 catgcgaaac tgacctaact acaagaggtc gagtttatgt ctatttatat ggaattacgc
      481 tgcgaactcc gaggggagtt accaattaat gagtcaaaat gttggtcaga agttgaccgc
      541 agcctgtggg caatggcatt aaacacccat gagtccacag aacagaccac aacagaactt
      601 ttaagcaagg ccacccatgc cggttggcaa agtattaatg gtgattggat atgccctggc
      661 tgccaagaaa atctggcatt aacagagcag atgcaggcag tcgccgaaag gcatgaccaa
      721 taacagtctt agtttcgcat gcgaaacttt tgaagaggat ttctgagatg acagcgaaag
      781 taatcacgat ttgttcacaa aaaggtggca tcggtaagac ctttgccaca acggaaatag
      841 cggcagagct gcagcgtcgg agctatcgga catgtgctgt tgatggggat tggatgagcg
      901 cgctgacgtc gcgccaattc cctgatggcc tgccgctgga aatagatgcc aatccttcag
      961 gacgcgttct tacgccgggc accgcacacg tcaatcgcat ctatggggaa gaaccctttg
     1021 agccgctgcg gctgcatgat ggccgtggat ttatgggggc gacggaagag ctgaacgata
     1081 tcaacaaccg caactccgat tgcatctacg atttccgcac ccgtttcgcg gaactgcgcg
     1141 accaatttga ctatatcctg attgactcgt cgccagtctg gtcaaacttg ctgatggcca
     1201 atcacttggt cgccgattat ctgttgatcc caacgctgtt ggaaaaaaca tcccgcctcg
     1261 gcgcagaaaa acacctgcgc acgttgaaga aaatcaagga gcggtataac ccagacctca
     1321 aactgctggg gatcgtggtt actcaggcga cggtggccaa ctataagaaa gaggtgctgg
     1381 gtggtcgtta tgcgagcgtc gataccgaga atctcaagat gttgctggag atcctggaag
     1441 agaatggttt cgggccggag catttgctgg agatcattcc ctatgttcaa accaaggtcc
     1501 gtgaagccat tgagctgaac tacaccatga aagaatacgc gccggacagc ccattggtgg
     1561 cgcgttacga cgcgctggtt gacactatca ttgcacggac agggggctga tacgatggcc
     1621 aaaaatttca aagcccaaat gcgtgaaaag cgcgaaaagg ccacgtcacc gcaaacgcag
     1681 gttgaaactc ccgctgtcga acgcgaactt gcgttaccgc gtaatgacct gaaccagaac
     1741 gacctgaagg tcaaccgggc tgagcacgag gaccctgcag gtcagataat tcgtgttcca
     1801 atcgatgatg tctatgctct tgagcaagtt cgccccgaag aagactttga agatgaagtt
     1861 atcgacggga tgaaacaaac ctacggcgac tttggcattt taatgccgcc tcgttgtttc
     1921 cctcgtgata agcgtgggta tcgaatctgg atgggtgaga cccgttggcg ttctgctaag
     1981 gcaaaagggg atctgttcat cgacatctat gtcggtgcac cgcctgaaaa tgagaagaag
     2041 cgtatctatg ggcagctgat cgaaaacctt caccagtctg gattaaaacc gttagccaca
     2101 gcaatacagc tgcaggcact tcgcgatgtg catggtgaaa ctcaggagtc gatgtcccgt
     2161 attttgggta aacccaaaac gttcatcagt aaacacatgc ggttgattga tgcgccagcg
     2221 ccggtcatcg ctctgctgaa agaaaaggtg accaaggatg tcgaactggt ctatacgctt
     2281 tgccaaatca acgatatcag tcctgatagc gttgataaac tggccaaact ggttcgggat
     2341 ggaaagctga cgcgtcaggg ggcgattaaa gagctgaacg tcctgaaggg taagccgcca
     2401 aagaacccgc cgccaccgcc gccaccaacc aactccggga cagaaactca ggaagagaac
     2461 cgtaacaaag atgtattgac ggaacagccg ggaacttcga cgaatcaaac cggcaacact
     2521 gccggcgaac agcagaatgc cggcaacgaa cagacccata agacgcaacc gctacagggc
     2581 gagtctaacg tcaacatcac tcgtgtcatg gtgaaatttg aggggggtga aggctatttg
     2641 ctgactgacc ggatacccga ggaatatggc caggtttgga tagcgctgcc gttaggtgaa
     2701 ctgtgcatcg atgccgcaga aatatctatc ctggggatca aagaggtata gatgagtaaa
     2761 gcgttgtttg cagacctggc ggagcagttt ttatccgtcg ctgtcaaaac ctgggtgata
     2821 cgttcagaat taccggccgc tgctgcggcc gatgttttgc ttaccactgc aatccagcag
     2881 ggcttcctgg aagaccgtgg acgcccttta ttgggtaata cactcaatga gtgggggaag
     2941 aacaagaaat acccgcgctg ggccatacag agtgctatag ctctcctttt ggctgatggg
     3001 tggaaacccg tcacccatcc tgaatgggct gcctatgcgg ctatacacgt tcagtctaaa
     3061 aaggggggat cgctgaccca actcctcgat gttttgccgg aagggctgga cattgatgtg
     3121 gcggcaggtt ggatctgcgc ggcgacggaa gacgctgcca gatatcatga gaggaaaaag
     3181 ctgcgatgac gttttctgat attgaggccg cactgtccag taccgggcgg cgtgcttacg
     3241 ccggcgtcat tttagctttc ctgcttgatg ctcacgatcg tggtgctggt ctggtcgaga
     3301 agcacacggt ggtggagaat gtcgggatca ccgacaccaa tgtttcggcg gtggcacgcc
     3361 gcctggagga acaaggaata atcgagatcc tctactggga tacaaaatcc gagagtccca
     3421 catggtccag cctacggaac ggtgcctggt cagcccccgg ttatcgtctg ctgccagctg
     3481 tactgcagtt gtatcgtcgt tgctgaatga caaggtttcg catgcgaaac tcacctgagg
     3541 atacagaatc gtaaaggaac cccgaatgaa actggtaacg aagaaatcac ccagaggtcg
     3601 actgttccgt gaattatcgt cggaagaggc gacggcggta ggcaacgttt gtggcagagg
     3661 acgcgtaact tcgctggctg aaaaacagtt ggctgaagcg gtgctgagaa gcatgcgtga
     3721 tcatgacctt tggctgtctt gtgggtgtcg cacgactgca gaagataggc cacttaacac
     3781 cgccagtaat cgcgacggca ctttgtttct ggtgaatttc agcggagagc acagctcgga
     3841 ttgtccgctg t
//
LOCUS       DADTFG010000244         3796 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.246, whole genome shotgun sequence.
ACCESSION   DADTFG010000244 DADTFG010000000
VERSION     DADTFG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.246"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..2420)
                     /locus_tag="J1138_004518"
     CDS             complement(<1..2420)
                     /locus_tag="J1138_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBA8456201.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCIRFLGTFLTDMNHIPSGVRHFIARQLAIHNITVLAEYGQRENTR
                     REHAAMIRQHYDYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWSRLAMIPSAVQRSQLETLLGPADSGRLSLLESLKKGP
                     VTISGPAFNEAIDRWKTLSNFGLHTDNLSAIPAVRLKNLARYAGMTSVFNIARMAPQK
                     RTAILVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSHIPRQKLADIIALVREIARPSDDNFHDEMVEQYGRV
                     RRFLPHLLNTVKFSAAPAGSATLNACDYLSREFSSRRQYFDDAPTEIISRPWKRLVVN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPREKLLQGADWQANRIKVYRS
                     LGHPTNPQDAIKSLGLQLDSRYRQVAARLGENEAVELDISGPKPRLTISPLASLDEPD
                     SLKRLSKQVSDLLPTVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQVWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFMSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGEAVFWRVDK
                     SANYGALD"
     gene            2584..3141
                     /locus_tag="J1138_004519"
     CDS             2584..3141
                     /locus_tag="J1138_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA8456202.1"
                     /translation="MRLFGYARVSTSQQSLDIQIKALKEAGVKANRIFTDKASGNSTD
                     RDGLDLLRMKVEDGDVILVKKLDRLGRDTADMIQLIKEFDSQGVAVRFIDDGICTDGD
                     MGKMVVTILSAVAQAERRRILERTNEGRQEAKLKGIQFGRKRTVDRSVVRALHLKGTG
                     AMEIARQLNIARSTVYKILEDMKTS"
     gene            3486..>3796
                     /locus_tag="J1138_004520"
     CDS             3486..>3796
                     /locus_tag="J1138_004520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456203.1"
                     /translation="MKVIDASLLEHVSGAGNFADNRNGGGRVNNNNNNNNGGNRGAPK
                     TAANDAAVAGLISAGVAAAAGTGPFGIAVAGIGAGVITAMGYPSNSPNNGGRDQGAFG
                     G"
BASE COUNT          945 a          953 c          971 g          927 t
ORIGIN      
        1 tcatccagtg caccgtaatt tgccgattta tccacccgcc agaataccgc ttcaccggca
       61 tcggccaggc gcggggaaaa ctggtatccc agcagccaga aaaggccaaa gacaagatcg
      121 ctggccccgg cggtatcggt cataatttct gtgggattta gcccggtttc ctgttccaga
      181 agtccttcca gtacaaaaat tgagtccctg agagttcccg gtataacaat gccatgaaag
      241 ccggaatact gatcggacat aaaattgtac caggtgatcc ccctgttatt gccaaagtat
      301 ttgcggttcg gcccggcatt gattgttctg accggcgtaa caaagcgcat tccgtctgca
      361 gaggccactt cgccaccacc ccagacctgt gccagtggta gcgttgcctg aaaatcaacc
      421 agcctggcat tcgcactggt gatagtttca gcccgcaggt agtttgcttt tgtccagttc
      481 agccggtgtc gggtcagggc tggaacattt gaccgtatca ggggttccag accgatattg
      541 caggcctcag ccatcagtac ggcgctgatg cttacaggca gatcgtcaac tctggcgctg
      601 gcttcgctgg cgtggaaaaa ctcatcagca aatccggtat gggcattgat ttcgagcaac
      661 agttctgtca aatccaccgt cgggagaaga tcactgacct gtttgctcag tcgtttcaga
      721 ctgtccggct cgtcaagact ggcgagggga gaaattgtca gccttggctt tgggccggaa
      781 atatccagtt caaccgcctc attttcgcca agacgtgcgg ctacctgcct gtaacggctg
      841 tccagttgaa gacccagcga ttttattgcg tcctgtggat tcgtcgggtg acccagggaa
      901 cgataaactt taattcggtt tgcctgccag tctgcgccct gtagtaattt ttcacgggga
      961 tctccccacc gattactgcc ggtgacgtag acatcccgcc gcctcaggct gtcctgcagt
     1021 ttactgagaa agcacagcgt gtatcccctt cgggtgatat gtttttcctt attaaccacc
     1081 agtcgtttcc atggacggct gatgatttcc gttggtgcat catcaaaata ctgccgcctc
     1141 gagctgaact cgcggctgag gtaatcgcag gcattcaggg tggcgctccc tgcgggagct
     1201 gctgaaaatt taacggtatt cagcagatgg ggcagaaaac gacgaacccg tccgtactgc
     1261 tccaccattt catcatgaaa gttatcgtct gaggggcggg caatttcgcg gacaagcgcg
     1321 atgatgtcgg ccagtttttg cctggggatg tggctgaaca cctcagcccg gattgattcg
     1381 tccggcgttt cctctttcag caggtacgaa catgcgctgg caagcgccag tgctgattta
     1441 tccagatcct tcagcgatcg gagccgtttt ttctgcccaa tctttctggc gtcacggatg
     1501 ataacagcca gcatggcatc cagaacgtcc agtgcatcat ccagcgccag cgtctcccat
     1561 gcaaggacaa aggcaaccag tatcgctgtc cttttctgcg gggccatcct ggcgatattg
     1621 aacaccgaag tcattcccgc ataacgagcg agatttttca gacgtacagc ggggatcgca
     1681 ctcaggttat cagtatgaag gccaaaattg ctcagcgttt tccagcgatc aattgcctca
     1741 ttaaacgccg gtccactgat ggttaccgga ccttttttca gtgattccag caaagacagg
     1801 cgaccggagt ctgcgggacc caatagcgtc tccagttgtg agcgctgcac ggcagacggt
     1861 atcattgcca gccggctcca caggcgcagc gttgcctttt cccttacctc tgaaatcagc
     1921 cgggtcaacg tggtcgcgcc ggggagaata attcggtgct gcatcagcca tcctgtcgcc
     1981 aggtcgaaaa gcagccccgg acgttcgttg cttatccagc tccgggtata taaaagacgg
     2041 gtaagacgaa aagtccaggg ccaggcaaat tcgcgataat cataatgctg acgtatcatc
     2101 gcggcatgct cacgacgggt attttctctc tgaccatact ctgccagcac ggtgatattg
     2161 tggatggcga gctgtctggc aataaaatgc cggacgccgg aaggaatatg attcatatcg
     2221 gtgaggaagg tgcccagaaa acggatgcat ccaatttgca gggcaatacc cagacggttg
     2281 tgatcacctc tgctttttcc gataaattcc ttgtctgcct catcaagatg aaaatagcgg
     2341 gccagctgaa gctcatctgg ttcaccggtg aatcggccat agctttcagt ctgctcagtg
     2401 gtcagaaaat cgacgggcat atcggcctcc ctgcctgatg ggcatttgtt aacaatttcg
     2461 caaccgtccg aaatattata aattgtcgga caccataaaa cagccggtct gagctgtcta
     2521 ttaaatcgct tttttgccat aatagacata gcatgtccga cattcgattt aaggtacatt
     2581 tttatgcgac tttttggtta tgcgcgtgtt tcgaccagcc agcaatctct cgacattcag
     2641 attaaagcgc tcaaagaagc tggagtgaag gccaatcgta tctttaccga taaagcatcg
     2701 ggcaattcta ctgatcggga tggcctagac ctgcttcgca tgaaagtgga ggacggtgat
     2761 gtcatcctgg taaaaaaact cgaccgactt ggccgcgaca ccgctgatat gatccaattg
     2821 ataaaagaat tcgactccca gggggtcgca gttcggttta ttgacgatgg tatctgtact
     2881 gacggtgaca tgggaaaaat ggtggtcact atcctctcag ctgtggcaca ggctgaacgc
     2941 agaaggatcc tggagcgcac gaatgagggg aggcaggaag cgaagctcaa aggcatccaa
     3001 tttgggcgta agcgcactgt cgacagaagc gtcgtgcgtg cccttcacct gaagggcacg
     3061 ggcgcaatgg aaattgctcg gcagctcaac attgcccgtt ccactgttta taaaattctg
     3121 gaggatatga agacttcgtg atagctccag atattcctaa ctcaactcct tcaacttaga
     3181 acgcgcgccg aagcaaatga taaatatgag tacaatacct accagttgtg gccatcttgc
     3241 accacaaagg aagtcgtccc ccaaacgctt actgagatcg aggcatgtgt aaaccgtagc
     3301 cccagaaaac caagctttga tccatgatgc tttattcatt gaatatcctt aacttaacgt
     3361 aaacaaaaag catgtatgtt atatctcact aatctaagct tgactcttct aggaaagtga
     3421 gctaatgatg ggttgcttta caaaagatta cacacagtta aacaaatgat caaaaggagt
     3481 tgtatatgaa agttattgat gcttccttgt tagaacatgt atctggcgct ggaaactttg
     3541 ctgataatcg taatggtggt ggcagagtta ataacaacaa caataataat aatggcggca
     3601 atcgaggggc cccaaaaact gcagcaaatg atgcagcagt tgctggttta attagtgcag
     3661 gagtagctgc agctgcaggc accgggccat tcggaatcgc agttgctggc attggcgccg
     3721 gtgtaattac cgcaatgggg tacccgagta acagtccaaa taatggtgga cgtgatcagg
     3781 gggcttttgg cggaaa
//
LOCUS       DADTFG010000245         3778 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.247, whole genome shotgun sequence.
ACCESSION   DADTFG010000245 DADTFG010000000
VERSION     DADTFG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.247"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..948
                     /locus_tag="J1138_004521"
     CDS             <1..948
                     /locus_tag="J1138_004521"
                     /inference="COORDINATES: protein motif:HMM:NF016136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456204.1"
                     /translation="AINIKIDDNLKNTGDAILRGLGLNATQYITLCWQYLAQQRKLPF
                     MTETKILTAADLTMTIATQFGDVLNQLHKIRDMLNSEDTDFAELSAAKLALARQAADI
                     QQNGWRLESLPEDLDASTPARRMLPRINYYLTGCDFALSDLPAELPVPTRVANEFGIS
                     LKAFETEFARLKSVLTNTGLLARPAPAREFVYRGENVTVSVVQPEDYQHGAWLVRMQA
                     KSLDRENALEDAALIFPALEGRVFLPGTVYGKSVRNSLTGKYEIGFCFLSGITEFHMY
                     SSGHEEEENPTSPDQVASRLAVTVDAYIQELLHEMSGHR"
     gene            complement(1764..1976)
                     /locus_tag="J1138_004522"
                     /pseudo
     CDS             complement(1764..1976)
                     /locus_tag="J1138_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2407..3585)
                     /locus_tag="J1138_004523"
     CDS             complement(2407..3585)
                     /locus_tag="J1138_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8456205.1"
                     /translation="MQRIHPGLLALSVTAFAIGMAEFIVVGILPEIATALTIDLPRAG
                     NLVGLYALALAVGTPLLVMAISGFPPKPVLTALILMFLAGSLLSALASSYPLFLTGRL
                     ITAVAHGSFFAIGATVAARIVPAGKAGKAIAVMFAGLTLAMVIGVPLGSFIGQIMGWR
                     VPLIMVSLLALLALLSTMLWLPGIRKSGSGNVREQLQALSHPAILIMMSITVLGFGAS
                     FAAFTFVIPILTTLSGFSSEVASFLLMVFGVATLIGNLAGGHFSSTRGWSVTLGIQIV
                     LLAVTLIALAVFLQWKVAVTGLLFLWGGLAFGLSPAVQQGMLSVAERYTPRAVGFASA
                     LNISAFNLGIFCGENIGSVLVHHNKLGYTPFAGAGICLLALLPLGLLVRYINRETPQK
                     "
     gene            complement(3590..>3778)
                     /locus_tag="J1138_004524"
     CDS             complement(3590..>3778)
                     /locus_tag="J1138_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016538810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBA8456206.1"
                     /translation="PGGKIYSLESTNGGLISFGGGVVLRDDQGAILGAVGVAGATVDS
                     DQQLALSGAAATSTAGRV"
BASE COUNT          956 a         1025 c          950 g          847 t
ORIGIN      
        1 gctatcaata taaagataga tgacaacctt aagaatacgg gagacgcgat cctgcggggg
       61 ctaggtctga atgcaacaca gtatattact ctctgctggc agtaccttgc ccagcaacgc
      121 aaattgcctt tcatgacgga gacgaagatc ttaactgccg ctgatctgac gatgaccatt
      181 gccacccagt ttggggatgt gcttaatcag ctgcataaaa ttcgggacat gctgaactct
      241 gaggacacgg attttgctga actgtctgcc gcaaaactgg ctctcgcccg tcaggcggca
      301 gacatacaac agaacggctg gcgactggag tccttgcctg aagatctcga tgcatccacc
      361 ccggcccgcc ggatgctgcc ccgaattaac tattacctga caggatgcga ctttgccttg
      421 tcggatttac cggcagaact gcctgtaccc actcgggtgg cgaatgagtt tggtatctca
      481 ctgaaggcgt tcgaaactga gtttgccagg ctgaaatccg tactcaccaa taccggtctg
      541 ctggcacgtc ctgcgccggc gcgcgaattc gtttaccggg gagaaaacgt cacagtttcc
      601 gtcgtacaac cggaggatta ccagcatggg gcatggctag tgcggatgca ggcaaaatca
      661 ctggatcggg aaaatgcgct ggaagatgcc gcactgatct tcccagcact ggaaggcaga
      721 gtgttcctgc ccggtaccgt ctacggtaaa tcggtacgta attcgctgac cggaaaatat
      781 gagataggat tctgttttct ctccggcatc acagagtttc atatgtactc cagcggacac
      841 gaggaggaag aaaatccaac ctcgcctgat caggtggcat cccgcctcgc cgtcacggtg
      901 gacgcttata tccaggagtt actgcatgag atgtcaggtc accgctgaca ataaatcccc
      961 agcagcggca acaggatggc agccaggcca tccgcaaact gagagacgag gagccagcct
     1021 tttaccggat gacaccggca acgtttgctg acgcctgtcc ggaccaggtc ccgcaactac
     1081 aaattgatga gttactggga cctttgaagt tacctgatgt tatgtttact ttcctctccg
     1141 ctgtcggcgg gccgcggcgg ggatactctg cgcggcgtcg cccatgcgcc aggaaaccct
     1201 tctttatgca gcctgtggcg caacatttta actctgtcat tactgatgtt atcggtatca
     1261 agtgataccg tgaccagtat tgccaggctg gtgtctgtga gggtaaaacc ggacgggaga
     1321 ccccagtggc tattcctggc cgcttcaatg tggtcaaaca gtttgcgcag gtagtccggt
     1381 acggcttccc ccctgccgtc aacgatggct tcctcgctcc ggtgtaaaaa tgctgtttca
     1441 ggaatttcca gagcggcaac gttcaggatc tgttcaagag aaggccaata tttaccgtca
     1501 tactgacagc gcagggcgtc gagaggctca tgcaaatacg aacgatagtg cccgttctgt
     1561 tctgaggccg cctcaatcag atcgacaatg tgcgtcgtac gctccagcga aaaggcgtta
     1621 cggttaagta tctggtcctg ctccgtcagc agcgcagaca aggcagccgc ctgcgcggcg
     1681 atcttctcac tcagatcagt caacctactg tagtcagcct tcagtcgcgt gttacgctcc
     1741 ttcgtaccat aagcgcccgc aaaaaaaatc tgctccagca gcgtgtggca ctgctgccag
     1801 ctgagccgct gctgcgcaat gttctcccat accggcatca tttccttttc acgggaaatc
     1861 agcctgtcca gcaacggcca gtgtggcttt cgcaaattat gctcatggag atcatcacgg
     1921 tacgccttta gtgtatcaag tacctcaccg gcagtacggg tatacatgta cttcatgtcg
     1981 tttcctcatc atttccgctt ccagcaatta tgtctaggca tcaccagaat gtcggtaaca
     2041 cagaagagga ttatttttag acgctgtgtt actccggttc cgactctgct ttttttcgga
     2101 tccgggcgag agataataat gattgatgca gattttagag taaatatgat ggattgttgc
     2161 ttcaagtgca ctattgatac taaagcagta aaatgcaatt tttatgcctt taatgagcac
     2221 aatcccgaaa taccgggatt gtgcagttgt ctttagtatt gataccggag cctgacggtt
     2281 aaccctgcag gatactgctt acccgcaggt ttttgcttac ggtattcccg gagtttctct
     2341 ttccctgagt tgaaacgcgg ggaattcaga atacaggaca cattattgtt gtttcatcgg
     2401 aaataattat ttctgcggag tctcacggtt gatatatctg accagtaacc cgaggggaag
     2461 cagggcaaga aggcatatcc ccgccccggc aaagggagtg tatccgagct tattatggtg
     2521 cacgagtaca ctgccgatat tttccccgca gaatataccc agattaaaag cagatatatt
     2581 cagggctgag gcaaatccga cggctcgcgg agtgtagcgt tccgccactg acaacatgcc
     2641 ctgctgtact gcaggagata atccaaatgc cagccccccc cacaggaaaa gtaacccagt
     2701 gacagccact ttccactgaa gaaaaacagc caaagctatt aatgttacgg ccagcaatac
     2761 aatctgaata cccagcgtta cagaccagcc ccgggttgat gaaaaatgac cgccagccag
     2821 gttaccaatc agtgtggcca ccccgaaaac catcaacaga aaacttgcga cttcggaaga
     2881 aaacccgctg agtgtagtca ggatggggat cacaaacgta aaggcagcaa aactggcccc
     2941 aaagcccagt actgtaatgc tcatcatgat caggattgcc ggatgtgaca gagcctgcag
     3001 ctgttccctt acattaccgg atccgctctt cctgatgccg ggcagccaga gcattgttga
     3061 cagtaatgca agaagagcca gtaaactcac catgattaat ggcacccgcc agcccattat
     3121 ctgaccaata aagctaccga gcggtacccc aataaccata gccagggtca gtccggcaaa
     3181 cataacggct atggccttac ctgctttccc tgcagggaca attctggccg ctaccgtcgc
     3241 accgatggcg aaaaaactgc catgcgcgac ggcggttatc agtcgcccgg taagaaaaag
     3301 agggtaactg ctcgccagtg ccgaaagcag gcttcctgcc agaaacataa ggatcagggc
     3361 tgtcagcacc ggtttggggg ggaagccaga tatcgccatc accagtaacg gtgtaccgac
     3421 ggccagggcc agcgcataca aacccacaag gttgccggca cggggtagat cgatggtcag
     3481 tgctgtcgcg atttcaggca gaatacctac aacgataaat tctgccatac caatagcaaa
     3541 cgctgtcacg gacaatgcca gcaaaccggg atgaatacgc tgcatagcct tacactctgc
     3601 ctgctgttga cgtggctgcg gctcccgaca gagcgagttg ctggtctgaa tctaccgtag
     3661 ccccggccac gccaacagcg ccaagaatag cgccctgatc atcccgcaga acaacaccgc
     3721 ctccaaaact gatcaagcct ccgttagtcg actccagtga ataaattttc ccgcccgg
//
LOCUS       DADTFG010000246         3750 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.248, whole genome shotgun sequence.
ACCESSION   DADTFG010000246 DADTFG010000000
VERSION     DADTFG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.248"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            197..2347
                     /gene="btsT"
                     /locus_tag="J1138_004525"
     CDS             197..2347
                     /gene="btsT"
                     /locus_tag="J1138_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBA8456207.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVIPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2465..2668
                     /locus_tag="J1138_004526"
     CDS             2465..2668
                     /locus_tag="J1138_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBA8456208.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2679..3635
                     /gene="yjiA"
                     /locus_tag="J1138_004527"
     CDS             2679..3635
                     /gene="yjiA"
                     /locus_tag="J1138_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBA8456209.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEVLCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
BASE COUNT          818 a         1033 c          996 g          903 t
ORIGIN      
        1 aacgacgcct ttgccgctca ccgccattcc tgaccgctta cgtccctaaa ataaccactc
       61 agttatttac cttactttac gcgcgcgtaa ctctggcaac atcactgcag gatagcggtc
      121 aatttacctc ctcaaacgca acgcaaacct agaacggctt cggccaacta ttaatcaata
      181 catgccaggt tttactatgg atactaaaaa gatattcaag cacataccct gggtgattct
      241 cggaatcatc ggtgcattct gcctcgcggt agttgcatta cgtcgggggg agcacgtcag
      301 cgccctgtgg atcgtggtcg cctctgtgtc ggtgtatctg gtggcgtatc gctactacag
      361 tctgtacatc gcccagaagg tgatgaaact cgaccccacg cgcgcgacgc ctgcggttat
      421 taacaacgac ggtctgaact acgttccgac caaccgttac gtgttgtttg gtcaccactt
      481 cgccgctatc gccggtgctg gtccgctggt gggtccggtt ctcgccgcgc agatgggcta
      541 cctgcctggc acgctgtggc tgctggcggg ggtcgtgctg gccggtgcgg ttcaggactt
      601 tatggtgctg tttatctcct ctcgccgtaa tggcgcatct ctcggtgaga tgatcaaaga
      661 agagatggga ccagtaccgg ggactatcgc gctgtttggc tgtttcttaa tcatgatcat
      721 catcctcgcc gttctggcgc tgattgtggt taaagccctg gccgaaagtc cgtggggtgt
      781 cttcaccgtt tgctcaaccg taccgattgc gctgtttatg ggtatctaca tgcgctttat
      841 tcgtccgggg cgtgtgggtg aagtctctgt cattggtatc gtgctgctgg ttgcctctat
      901 ctacttcggt ggcgtgattg ctcacgatcc gtactggggt ccggcactga cctttaaaga
      961 caccaccatt accttcgcgc tgattggcta tgcgtttgtt tccgcactgc tgccagtgtg
     1021 gctgatcctc gcaccgcgcg actatctggc aaccttcctg aaaatcggcg ttatcgtcgg
     1081 cctggcgctg ggtatcgtgg tgttgaaccc ggaactgaaa atgcctgcca tgacccagta
     1141 cattgacggt actggcccac tgtggaaagg cgctctgttc ccgttcctgt tcatcaccat
     1201 cgcctgtggt gcggtatctg gcttccacgc gctgatctct tcgggtacga cgccgaaact
     1261 gctggctaat gaaaccgacg cgcgtttcat cggctacggt gcaatgctga tggagtcctt
     1321 cgtggcgatt atggcgctgg ttgctgcgtc catcatcgaa ccgggtcttt acttcgcgat
     1381 gaacaccccg cctgctggcc tcggcatcac catgcctaac ctgcatgaaa tgggtggcga
     1441 gaacgcgccg atcatcatgg cacagctgaa agacgttacc gcacacgcgg cagcgaccgt
     1501 cagctcctgg ggcttcgtga tttcgccaga gcagatcctg caaaccgcga aagacattgg
     1561 tgagccttct gtcctgaacc gtgcaggtgg tgcgccaacg ctggcggtag gtatcgctca
     1621 cgtgttccac aaagtgctgc cgatggctga catgggcttc tggtatcact tcggtattct
     1681 gttcgaagcc ctgttcatcc tgaccgcgct ggatgcgggt acccgttctg gccgctttat
     1741 gctgcaagac ctgctgggta acttcatccc gttcctgaaa aaaaccgact ctctggttgc
     1801 cggtatcatc ggtactgcgg gctgtgtggg tctgtggggc tacctgctgt atcagggcgt
     1861 ggtcgatccg ctgggcggcg ttaagagcct gtggccgctg ttcggtatct ccaaccagat
     1921 gctggcagcc gtggcgctgg tactgggcac cgttgtactg attaagatga agcgcaccca
     1981 atacatctgg gttaccgtta tcccagcggt atggctgctt atctgcacca cctgggcgct
     2041 gggcctgaaa ctgttcagca ccaacccgca gatggaaggc ttcttctaca tggcaagcca
     2101 gtacaaagag aagattgcta acggtactga cctgacggcg cagcagattg ccaacatgaa
     2161 ccacatcgtt gtgaacaact acaccaacgc aggcctgagt attctgttcc tgattgtggt
     2221 gtacagcatc atcttctacg gtttcaaaac ctggctgaag gtgcgtaaca gcgacaaacg
     2281 tactgacaaa gaaacaccgt acgttccaat cccggaaggc ggcgtgaaga tctcttcgca
     2341 ccactaaccg gttgccttat ccggcttaca aaatgtctat cgtaggccgg ataaggcgtt
     2401 tacgccgcat ccggcaaatc cccagcccgg accttccggg ctttacgcat atctggaagg
     2461 caaaatgttt ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt
     2521 gattggcatt ccagactacg acaactacgt cgagcatatg aagaccaacc atcccgacaa
     2581 gccgtacatg agctatgaag aattcttccg cgaacgccag aacgcgcgct acggcggcga
     2641 tggtaaaggc ggtatgcgct gttgttaatg gagagagcat gaacccgatt gcagttaccc
     2701 tactcaccgg ttttttaggc gcaggaaaaa ccaccctgct gcgccatatt cttaacgaac
     2761 aacacggcta caagattgcc gtgattgaaa acgaattcgg cgaagtctcc gttgatgatc
     2821 aactgattgg cgatcgcgcc acgcagatca aaacgctgac caacggctgc atctgctgtt
     2881 cgcgctccaa cgagctggaa gacgccctac tcgacctgct ggataatctc gacaagggca
     2941 atattcagtt cgaccgtctg gtgattgaat gcaccggcat ggccgatccc ggcccgatta
     3001 ttcagacctt tttctcccat gaagttttat gccagcgtta cctgttggac ggcgtgattg
     3061 cgctggtgga tgcggtacat gccgatgagc agatgaacca gttcaccatc gcccagtccc
     3121 aggttggcta cgccgaccgt attctgctga ctaaaaccga cgtcgcaggc gaagcagaaa
     3181 aactgcgtga acgcctggcg cgcatcaacg cccgtgcacc ggtctacacc gtcacccacg
     3241 gcgacatcga cctgggcctg ctgttcaaca ccaacggttt tatgctggaa gaaaacgtcg
     3301 tcagcaccaa gccacgtttt cactttatcg cggacaaaca aaacgatatt tcgtcgattg
     3361 tggtggaact ggattacccg gtagatatca gcgaagtttc ccgtgtgatg gaaaacctgc
     3421 tgctggaatc ggcggataaa ctgctgcgtt acaaaggaat gctatggatt gacggcgaac
     3481 ctaatcgcct gctgttccag ggcgtccaac gcctctacag cgccgactgg gaccgcccgt
     3541 ggggcgatga aaaaccgcat agtacgatgg tgtttatcgg gattcagttg ccggaagatg
     3601 agattcgggc agcgtttgcg gggttgagga agtaataccc ttaacctact aatacgaagc
     3661 ggacgaggat aaaaccctat cattttccgt tcgcttcact attttatttc gttccaacac
     3721 ccataaaaat taaaaatata attctgccat
//
LOCUS       DADTFG010000247         3597 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.249, whole genome shotgun sequence.
ACCESSION   DADTFG010000247 DADTFG010000000
VERSION     DADTFG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.249"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(373..675)
                     /locus_tag="J1138_004528"
     CDS             complement(373..675)
                     /locus_tag="J1138_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456210.1"
                     /translation="MSVYKNAKSQMTTIRVPHDVMEGMESVKLDGESNAGFIVTAMRG
                     EIARRQTEGSGENPLVSSLDALAKVEQIGIKAAEEIGQLVAVAREELQRRKAKESE"
     gene            884..1249
                     /locus_tag="J1138_004529"
     CDS             884..1249
                     /locus_tag="J1138_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBA8456211.1"
                     /translation="MAGTQHTQTHPKFIYTFLALHHDRMADGATTVHVAADTLVDARK
                     MVKEMGYTAAFWKGREENTLFIQKCENNFIWRFIALSTAQPRVIHIEAASEQEARQQS
                     PTGYVMIFAARIRQEVCHE"
     gene            1242..1466
                     /locus_tag="J1138_004530"
     CDS             1242..1466
                     /locus_tag="J1138_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456212.1"
                     /translation="MNDLYFKVLTHAENALVCGKNMREILSTWLDGTTNAEHDERDAN
                     LAGALITLLDPVIKELDEAIKIHDQSYTGE"
     gene            1470..1757
                     /locus_tag="J1138_004531"
     CDS             1470..1757
                     /locus_tag="J1138_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBA8456213.1"
                     /translation="MKNKFSGFIASGQTHSKISLGDIFKDSYGYRVKIISVDDRRVSY
                     LRDGYDFECVMPRQQFERDFILVKNCKTDNQRRAAGYIRKIRAMLVAGGNK"
     gene            1754..3574
                     /locus_tag="J1138_004532"
     CDS             1754..3574
                     /locus_tag="J1138_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBA8456214.1"
                     /translation="MKRAPNLKYQPRDKMTEVIIFAGSDAWSHAKEWNEWAGKHIAAD
                     DTPPVILGTEQLENLDDMQIIDEGRHYVRVYRAGKITEKSLTKVATLLAIAGVKEARC
                     YRSFVDREPEDWTPRLVGLKAEAEHGESLVIELPVKKAERKNDERASSLALNQMGASQ
                     RGEVLLAHYGGELAINADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAI
                     KSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAE
                     GETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKS
                     VMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITG
                     GDKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERD
                     SMLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYL
                     MASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEY
                     GREYMKRKTKHGLRSNVTLTEESEDWMPSCVSVTNDDSKN"
BASE COUNT          988 a          734 c          928 g          947 t
ORIGIN      
        1 tggctatttt ttttagattc atagtattct aactccctta acttatttat tactttctct
       61 ttatgataag agaggggaat gacttggtaa agtgatttgt cattaataat gataatcctt
      121 gcgataaata tttctgcaag ttatttatct gtttgttgct atcttgttaa gcgttgggtg
      181 tggtttatgt ttggagcgac tttatttaat cgtgatttta tggatctata tttatatcct
      241 gtaagataag cgcatgttag ttcaggagta gtgtcagata aaattattcg gagtagaact
      301 accttttctg tttattgcca gtatttacat agcaatgcgc cgtagttact tacatcacgg
      361 cgctgatagt aattattcag attctttggc tttacgccgc tggagttctt cacgagcgac
      421 ggctacgagt tgcccgattt cctccgctgc cttgatgccg atttgttcga ccttagctaa
      481 ggcatccagt gaagacacga ggggattttc tccgcttcct tctgtctggc ggcgggcgat
      541 ctcaccgcgc atggcggtta ctatgaatcc ggcgttgctt tcgccgtcca gttttacgga
      601 ttccatgccc tccataacat cgtggggaac cctgatcgtt gtcatttgcg attttgcatt
      661 tttgtataca gacatagtta atcaccaatt tgttatgtgt atatcactat acacaaaagt
      721 gagatataaa aacacttgca atgtatatca ccacaatata atgtatatct cacattatgg
      781 tcgctatgta tctcacaatg atcgattcat aaaaacgacg aaacccggca gtgcgcgaac
      841 actaaccggg cttctaacca aaccgttaaa cgaggtaacg attatggctg gaacacagca
      901 tacccaaact caccctaaat ttatatacac ctttctggca ctacaccacg accgcatggc
      961 agatggagca actacggtac atgttgccgc tgacacgctg gttgatgccc gcaagatggt
     1021 taaggagatg ggctatactg cggctttctg gaaagggcgt gaagaaaaca cgctgtttat
     1081 tcagaaatgc gaaaacaatt tcatctggcg ttttatcgcc ctgagcaccg cacaaccacg
     1141 cgtaatacac atcgaggccg ccagcgaaca ggaagcccgc cagcaatccc caactggcta
     1201 cgtgatgata ttcgccgccc gtattcgtca ggaggtgtgc catgaatgat ctttacttta
     1261 aagtgctgac acatgctgaa aacgcgctcg tttgtggcaa aaatatgcga gaaatcttat
     1321 caacctggct tgatgggaca acaaatgcgg aacacgatga acgggatgct aatttagctg
     1381 gagcgttaat tacgttactt gatcctgtca tcaaagagct ggatgaagct ataaaaatac
     1441 acgaccagag ctataccgga gaataaaaaa tgaaaaataa attttctggc tttattgcca
     1501 gcggtcaaac tcattcaaaa atcagccttg gggatatttt caaagacagc tatggctatc
     1561 gggtaaagat tatttcggtt gatgatcgtc gtgtctctta tttgcgtgat ggttatgatt
     1621 ttgaatgtgt tatgccgcgt cagcagttcg aaagagattt cattctggta aaaaattgca
     1681 agacagataa tcagaggcga gccgcaggct atatccgtaa aattcgggca atgttagttg
     1741 ccggaggtaa caaatgaaac gtgctccgaa cttaaaatac caaccgcgcg acaaaatgac
     1801 ggaagtcatc atttttgctg gcagtgatgc ctggagccat gcaaaagaat ggaatgaatg
     1861 ggcaggtaag catattgcag cagatgatac accaccagtc attctgggta cggaacaact
     1921 ggaaaacctg gatgatatgc aaattatcga tgaaggccgt cattatgtgc gtgtttatcg
     1981 tgccggaaag attacagaga aaagtctgac gaaggttgcg acattacttg ctattgcagg
     2041 cgtaaaggaa gcacgttgtt accgtagctt tgttgatcga gagcctgaag actggactcc
     2101 gcgccttgtc ggcctaaaag ctgaagcgga gcatggggaa agtctggtga ttgaactgcc
     2161 agtgaagaag gcagagcgca aaaatgacga gcgtgcttca tctttggcgt tgaatcagat
     2221 gggggccagc cagcgcggtg aagttctcct tgcacattac ggcggcgaac tggcaatcaa
     2281 tgccgactct gacaccgttc atcattacaa cggcgttgta tgggagccgg ttcaggataa
     2341 agagttacag cgtgctatgg cgcagatttt cattgatgcg gagatcagct attcgcagaa
     2401 cgctattaaa tcggcggtag ataccatgaa gttaagtttg cctgtaatgg ggaatacagc
     2461 ccgtaacctg attggattca gtaacggggt atttgatacc agaactggta attttcggga
     2521 gcataacaaa aacgactggt tgttaattgc cagtgaatta cctttcagcc caccagcaga
     2581 gggggaaacg ctggcaacac atgcgccgaa tttctggaag tggttgcgcc gttcggtggc
     2641 tgagaatgac cgcaaggcag atcgcgtact ggcggcatta ttcatggtgc tggcgaaccg
     2701 gtacgactgg cagttattca ttgaggtaac agggccgggg ggaagtggta aaagtgtgat
     2761 ggcggagatt tgcaccatgc tggcgggtaa ggctaataca gtatcagcaa gcatgaaggc
     2821 gctggaagat gcaagggaac gtgcgttagt ggttggcttt tcgctgatta tcatgccgga
     2881 tatgacccgc tacgctggtg atggggcagg gattaaggcc attacaggcg gtgacaaggt
     2941 ggcaattgac ccgaaacaca aagcccccta ttcaacgcgt attccggcag tagtgctggc
     3001 ggttaacaat aacgccatgt cattcagtga ccgcagcggg gggatctcac gtcgtcgggt
     3061 gatattcaat ttttcggaag ttgtaccgga gaacgaacgc gattcgatgc tggcggaaaa
     3121 aatagaaggt gagctggcgg tagtgattcg tcatctgctt acaaggtttg ctgatcagga
     3181 cgaagccaga cgcctgttat atgagcagca gaaatctgaa gaagcactgg cgataaagcg
     3241 agagggggat tcgctggtgg acttctgcgg ttatctcatg gcatcggtaa tgtgtgatgg
     3301 cctgttagtg ggtaatgctg aaattgtgcc attcagccca cgcaggtatc tctatcatgc
     3361 ctatctggct tatatgaggg cacatgggtt tggtaaacct gtaacactga cgcgcttcgg
     3421 taaagatatg ccgggggcaa tggcggaata tggcagggag tatatgaaac ggaaaacgaa
     3481 gcacggtttg cgttcaaacg tgacactgac ggaggaatca gaagactgga tgccatcatg
     3541 tgtatcggtc actaatgacg atagcaaaaa ttaaacttat ggaataactg ttcacca
//
LOCUS       DADTFG010000248         3490 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.250, whole genome shotgun sequence.
ACCESSION   DADTFG010000248 DADTFG010000000
VERSION     DADTFG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.250"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..1718)
                     /locus_tag="J1138_004533"
     CDS             complement(<1..1718)
                     /locus_tag="J1138_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBA8456215.1"
                     /translation="MLMKRHLNTSYRLVWNHITGAFVVASELARARGKRAGVAVALSL
                     AAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDDNTG
                     GQQIARGGTTRNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQAYGTVLNGGEQWI
                     HAGGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPESENVSTGQMVGGIAESTTI
                     NKNGRQVIWSSGIARDTLIYTGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGG
                     WQVVKAGGTAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGTNRLGAFS
                     VVEGKADNVVLENGGRLDVLSGHTATRTLVDDGGTLDVRNGGTATTVSMGNGGVLLAD
                     SGAAVSGTRSDGTAFRIGGGQVDALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSL
                     AGTTTLNNGATFTLAGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTT
                     LTQKAVNLNEGTLTLNDSTVTTDIIAHRGTALKLTGCTVLNGAIDPTNVTLASGATWN
                     IPDNATV"
     gene            complement(2043..2915)
                     /locus_tag="J1138_004534"
     CDS             complement(2043..2915)
                     /locus_tag="J1138_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBA8456216.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIKPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT          780 a          971 c          930 g          809 t
ORIGIN      
        1 accgtggcgt tatcgggaat attccaggtg gcacctgagg cgagggtgac attcgtgggg
       61 tcaatggcac cgttcagcac ggtgcagccg gtcagcttca gggccgtgcc gcgatgagcg
      121 atgatatccg tggtgacggt actgtcgttc agcgtcagcg tgccttcatt caggttgacg
      181 gctttctggg tgagtgtggt gttgctgaca gtgagtgtgc cacttcctga tttttccacc
      241 ctgccgttac cggtgagagc gcctccctgc aggagtgcat caccttcacg gatggtcagg
      301 gtatcgttgt tcaccgtctt cccggcaagg gtaaacgtgg caccgttatt cagcgtggtg
      361 gtgcccgcca gcgagccccc tctggcagtg aacagtccgc catttaccgt ggtatccgtg
      421 gccgtatcac cggcgttcag cgtgaatgaa ctgccttttt ccagcatcag ggcatccacc
      481 tgaccgcccc cgatacggaa tgccgttccg tcgctccggg taccactgac agcggccccg
      541 gaatcggcca gcagtacccc gccatttccc atggatacgg tagtggcggt accaccgttg
      601 cggacatcca gcgttccgcc atcatccaca agggttctgg tggctgtgtg tccgctcagc
      661 acatccagac gtccgccatt ttccagtacg acattatcag ctttaccctc cacaacagag
      721 aatgctccca ggcggtttgt accggtgaca gttgcagcag tgctggtaac cagtgcacct
      781 cccgtgttct gggtgactgc agtggcttcc ccgccggcat gaaccctcag ctcgccgttc
      841 tgatttatgg tggtgttacc ggcagtacca ccggccttaa caacctgcca gcccccctcg
      901 ttaattaccg tgttcagtgc aagtccatac ttgtgcacat actggttccc tccctccagt
      961 cgggtgttat gtgcctctcc gtgtaccgtc tggtcacctc ctgtgtaaat gagagtgtcc
     1021 cgggctatcc ccgaagacca gataacctgc cggccatttt tgtttatggt ggtggattca
     1081 gcaatccccc cgaccatctg accggtggac acattttcag actccggacc accttctgca
     1141 ccggtgttga cgatggttcc ggttgcctgc ccgccatgtt taatggtctg gtagccagac
     1201 tggttaatca ccgttccgga tgcactgccg cctgcatgta tccattgttc accaccgtta
     1261 aggacagtac cgtaagcctg cccgcgcaga ttctgtcctc caccggcact gataaccgta
     1321 tccgtggcac tgccacctgc catgacatcc tgcagaccgt ttgcggtgac cgtggtgttt
     1381 ctggtagtgc caccccgggc tatctgctga ccgccggtgt tgtcatcaga gtccggcccc
     1441 agctcaagcc cggtactgac agtcacgcca tcagctgttc cggatacaaa ctggttgtca
     1501 tggtttacca gtgttccgcc attcactgtt tcacccgggt gcacaacgat gtcagcagcc
     1561 agcaccggga gtgacgtgac tgcggcaaga gacagtgcaa ccgccacacc ggcgcgtttc
     1621 ccccgtgcac gggccagttc ggaggcaacc acgaaagcgc ctgtaatgtg attccatacc
     1681 agcctgtagc tggtattcag atgtcgtttc atcagcattt ccttatcaga gtgagggtga
     1741 ataaaaagcc ggtatccaca aatgtggata ccggcaggtg aagggttaca gtgccgcaga
     1801 tccggctgat ggaagtttct gacgtgtggc tctgttgttc cctgaatctc atgtctgtgt
     1861 gtctgcatac gttgctaacg ggtgttatta ttagcataac gatcgatata aacgatcgat
     1921 taaataggta tcgatcgttt tattctatta cctgattgtt gcacggacaa gaaaggtggc
     1981 aatgaaaacc gcgctttttg tgagatggct cagaggcagg gagggcatca tctacaacag
     2041 gttcagaaga aaatccagtt ccataccgcg cgggcaacag agaccacggt gtcacgaaca
     2101 gcgcgcaggg ccgtacggac aacagaggca acacagacgc tctccgctgt gtcaaatatc
     2161 cggtccaccg caccggtaaa ctgttcacgg gcctgagagc ggacagactc cgtgcgcagc
     2221 ccgtcctgca ggcgggtcat caggggactg gcggcatggt cgggaagcgc tgtcatgagc
     2281 gcactgacca gcgtatccag ctcccagccg gtgcgggccg atacggcaac aaccggatgt
     2341 acgggccgga acagacggaa taccgcctcc gttttttcgc gaatattctg tgcctgtgcg
     2401 ggagaaggct taataccggc catatcccat tcatggcagg gctccgtttt atcggcctgc
     2461 gtcaccacaa acagtacccg ctgatgtccc cggtgcagga tgtgtcgcca gaaatactca
     2521 tccacagaca gggcacggtc atcggcttta atcagccaca gtaccaggtc cagttctggc
     2581 agaatgtcac ggtacagggc ttcatactct gcatccctgt cccggctctc gcccaccccg
     2641 ggcaggtcag tgataaccat gctgtgaccg tggccactca gacggaagcg ccgcacttcc
     2701 cgggtgccag cgtgaacatc actgaccggg gtgacctccc cctgaaacag tgcattacag
     2761 agtgaggatt taccggcccc gcttttcccc ataatgccaa tcacgggttc gtggctggtg
     2821 agtttgcgca gatgttccag gatgtgacgg gaaagtgaat aaggcaggga ggagagcggt
     2881 ttttcaattg cctcaatggc atcagacgga ttcatacatg tattcccggt aaaaaaagac
     2941 aaaaccccgc gcaccggaaa ggtggcgggg gattcacgga gataatatct gtctgaaatt
     3001 atttaggatg tgccagacat acagcaaagg agagaagtat tcggccacgc ctccctgcca
     3061 tgaataaccc ggagtccacc gacttactta cgggctacct gagccacgcg tttgctgtag
     3121 aatagcccct gatgattcat acctgaacca ggaaaacgaa ttaaaaatga caggcattca
     3181 ataacgagca gggctgcaac accagatagg gcaaatggtc aatgatgtca gagggtctgt
     3241 cgacggatgg gattttaaac agtacatttt gggcgcactt ttcaaccgtt ttatcagcga
     3301 aaatttttcc agccatatgg aatattgtcg atcaagatgt tgtaaaaaga tccagaaaaa
     3361 atatttcaaa ggcatgaaag aacttgctga caagaagttt attgcagaga ccatgataca
     3421 gaactattac tttattaacc caggctatat gtttaatggt catcgcttga cctatatgaa
     3481 gacctattac
//
LOCUS       DADTFG010000249         3469 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.251, whole genome shotgun sequence.
ACCESSION   DADTFG010000249 DADTFG010000000
VERSION     DADTFG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.251"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            165..1130
                     /gene="dusB"
                     /locus_tag="J1138_004535"
     CDS             165..1130
                     /gene="dusB"
                     /locus_tag="J1138_004535"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBA8456217.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1156..1452
                     /gene="fis"
                     /locus_tag="J1138_004536"
     CDS             1156..1452
                     /gene="fis"
                     /locus_tag="J1138_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBA8456218.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1538..2422
                     /gene="yhdJ"
                     /locus_tag="J1138_004537"
     CDS             1538..2422
                     /gene="yhdJ"
                     /locus_tag="J1138_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBA8456219.1"
                     /translation="MRTGCEPTRFGNEAKTIILGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            2506..2685
                     /locus_tag="J1138_004538"
     CDS             2506..2685
                     /locus_tag="J1138_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HBA8456220.1"
                     /translation="MIRRYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2688..3350)
                     /gene="acrS"
                     /locus_tag="J1138_004539"
     CDS             complement(2688..3350)
                     /gene="acrS"
                     /locus_tag="J1138_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBA8456221.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDYLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMACYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT          957 a          792 c          813 g          907 t
ORIGIN      
        1 tttctaatat gatgattttt atgagtaatt atcgcgctac gctcatttta aatgcaattc
       61 tttgatccat ctcagaggat tggtcaaagt ttggcctttc atctcgtgca aaaaatgcgt
      121 aatatacgcc gccttgcagt cacagtatgg tcatttctta actcatgcgc atcggacaat
      181 atcagctcag aaatcgcctg atcgcagcgc ccatggctgg cattacagac agaccttttc
      241 ggacgttgtg ctacgagatg ggagccggat tgacagtatc cgagatgatg tcttctaacc
      301 cacaggtttg ggaaagcgac aaatctcgtt tacggatggt gcacattgat gaacccggta
      361 ttcgcaccgt gcaaattgct ggtagcgatc cgaaagaaat ggcagatgca gcacgtatta
      421 acgtggaaag cggtgcccag attattgata tcaatatggg ttgcccggct aaaaaagtga
      481 atcgcaagct cgcaggttca gccctcttgc agtacccgga tgtcgttaaa tcgatcctta
      541 ccgaggtcgt caatgcagtg gacgttcctg ttaccctgaa aattcgcact ggctgggcac
      601 cggaacaccg taactgcgaa gagattgccc aactggctga agactgtggc attcaggctc
      661 tgaccattca tggccgtaca cgcgcctgtt tgttcaatgg agaagctgag tacgacagta
      721 ttcgggcagt taagcagaaa gtttccattc cggttatcgc gaatggtgac attactgacc
      781 cgcttaaagc cagagctgtg ctcgactata caggggcgga tgccctgatg ataggccgcg
      841 cagctcaggg aagaccctgg atctttcggg aaatccagca ttatctggac actggggagt
      901 tgctgccccc gctgcctttg gcagaggtta agcgcttgct ttgcgcgcac gttcgggaac
      961 tgcatgactt ttatggtccg gcaaaagggt accgaattgc acgtaaacac gtttcctggt
     1021 atctccagga acacgctcca aatgaccagt ttcggcgcac attcaacgcc attgaggatg
     1081 ccagcgaaca gctggaggcg ttggaggcat acttcgaaaa ttttgcgtaa acagaaataa
     1141 agagctgaca gaactatgtt cgaacaacgc gtaaattctg acgtactgac cgtttctacc
     1201 gttaactctc aggatcaggt aacccaaaaa cccctgcgtg actcggttaa acaggcactg
     1261 aagaactatt ttgctcaact gaatggtcag gatgtgaatg acctctatga gctggtactg
     1321 gctgaagtag aacagcccct gttggacatg gtgatgcaat acacccgtgg taaccagacc
     1381 cgtgctgctc tgatgatggg catcaaccgt ggtacgctgc gtaaaaaatt gaaaaaatac
     1441 ggcatgaact aattcaggtt agctaaatgc ttgattaaaa aggcgctact cggcatgggg
     1501 aagcgccttt tttataggtg tcacaaaggg agtgaccatg agaacaggat gtgaaccgac
     1561 ccgatttggt aatgaagcaa agaccatcat cctgggtgat gcattaaccg aacttaaaaa
     1621 gctccccgcc gaaagtgtcg atctgatctt tgccgaccca ccgtataaca tcggtaaaaa
     1681 ttttgatggt ctgatcgaag cctggaaaga agatctgttt atcgactggc tgtttgaagt
     1741 gattgcagag tgccatcgcg ttctgaaaaa gcagggcagc atgtacatca tgaatagcac
     1801 ggaaaacatg ccctttatcg atctccagtg ccgcaagctt tttaccatca aaagtcgcat
     1861 cgtctggtca tatgacagtt ctggagtgca ggcgaaaaaa cactacggct ccatgtacga
     1921 acccatcctg atgatggtga aagacgcaaa gaactacacg ttcaacggtg atgctattct
     1981 ggtagaagcc aaaaccggat cgcagcgcgc gttgatcaat tatcgcaaaa atcctccaca
     2041 gccttacaat catcaaaaag taccgggtaa cgtctgggat tttccgcgcg tgcgttattt
     2101 gatggatgaa tatgaaaacc acccgacgca aaaaccggaa gccttgctga aacgcattat
     2161 tctcgcctct tccaacccag gcgatatcgt tctcgacccg tttgccggta gctttactac
     2221 cggtgccgta gccgtcgcca gcgggcgaaa attcattggt attgagatca acagcgagta
     2281 catcaaaatg ggtcttcgac ggctggatgt cgcgtcgcat tactctgcgg aagaactggc
     2341 gaaagtgaaa aaaagaaaga ccggcaacct gtcaaaacga agccggttaa gcgaagttga
     2401 ccccgatctc attacaaagt aaaggaagtg taagcctggt tttcagatta ttcatttcgt
     2461 gtatatttcc tgccagactt ggttaaacat gcacaggctc aggtaatgat tcgcaggtat
     2521 tggtggctcg tcgttttcgc tgtcttcgtt tttctgtttg atactttact gatgcagtgg
     2581 attgaactgc tggcaacaga aacagacaaa tgccgcaata tgaactcagt taatccacta
     2641 aaactggtta actgtgacga actgaatttt caggacagaa tgtgaattta tatgacactt
     2701 aattcattcg ttttatgaat taatttcgtt atgttttcat ctggcatgaa cattcttaat
     2761 acgttatcga ccagtgcggg tgcttgttta taaaggtcat aacacgccat attcattaac
     2821 cagttttgaa caattccgct gaaggcacca tcaataataa tcatcacaac atctaaatcg
     2881 agattatttg ctacacaacc ttgttgctga cacgcctgca atacttcgcg gagagtctgc
     2941 ggattaaagc ccatcttttc gcgtatcact ccctcggcca gcatctcatc attaaattca
     3001 catttgtgat ataagatttt cagcaacgcc tgctggcggg gaattttggc aatatattgc
     3061 aaaccgacaa tcaatttttc acgcaattgc tgaaacgggt catgctctaa cccagcggtc
     3121 aagtaatcct ggattaactc ccgcaatgaa ggctgttgca accacatctc attaaacagt
     3181 tgagtcttgt tttcgaagtg ccagtagata gcgccacgcg taacgttagc agcgtcggca
     3241 atgtcgttga gcgtcgtctt gcttacgcca tgctgcgcaa actgggcgat ggcagtttca
     3301 atcagttctt gccgggtctt cagggcttca gctttggttc tttttgccat gattaattat
     3361 tcaggaaata aatatattca acacaggatg agaaaatagc gaaggttaat ctatcaccta
     3421 atgtgtattt atacgagagg ctaatattgc gttgctataa atcgtcaaa
//
LOCUS       DADTFG010000250         3372 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.252, whole genome shotgun sequence.
ACCESSION   DADTFG010000250 DADTFG010000000
VERSION     DADTFG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.252"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(323..398)
                     /locus_tag="J1138_004540"
     tRNA            complement(323..398)
                     /locus_tag="J1138_004540"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(363..365),aa:Asn,seq:gtt)
     gene            complement(499..2142)
                     /gene="yeeO"
                     /locus_tag="J1138_004541"
     CDS             complement(499..2142)
                     /gene="yeeO"
                     /locus_tag="J1138_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBA8456222.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQEPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2147..2222
                     /locus_tag="J1138_004542"
     tRNA            2147..2222
                     /locus_tag="J1138_004542"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2180..2182,aa:Asn,seq:gtt)
     gene            2384..>3372
                     /locus_tag="J1138_004543"
     CDS             2384..>3372
                     /locus_tag="J1138_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012541215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA8456223.1"
                     /translation="MSLNDAKIRSLKPTDKPFKVSDSHGLYLLVKPGGSRLWYLKYRI
                     NGKESRIALGAYPAVSLSDARQQREGIRKMLALNINPAQQRAAERGSRMQEKMFKSVA
                     LEWHSSKKKWSQNTADRVLARLNRHVFPTIGHLPVTELKSRHFIELLKGIEEKGLLEV
                     ASRSRQHLSNIMRYAVHQGLIEINPAANLDGVTASPARRHYPTLPLERLPELLERIDS
                     YHQGRELTRLAVLLTLHVFIRSSELRYARWTEINFRNRIWTIPATREAIAGVRYSSRG
                     AKMRTPHIVPLSEQVISILKRIKEISGGYELVFPGYHDPYKPMSENTINKALR"
BASE COUNT          971 a          882 c          727 g          792 t
ORIGIN      
        1 gaaataatat ttattaaaat tccaggcctt ctttttctga ttacagaagg caaagtggca
       61 attacgtata gtttcccgat aaagacgtga cagcaacatc ccacataagg catttttctc
      121 tttatctttg tacggtgctt catggaacag agtttttgac cttgcgaatc gtgatgtctg
      181 ttactgaagc gtgagactcg attaaacaca cgaaacacag aaatcaaaaa acccggtcac
      241 ttttttacaa ggtaaccggg taaaaataat ttttattttt taactgtctt gagactcata
      301 aagatgtctc aagactaaaa tttggctcct ctgactggac tcgaaccagt gacatacgga
      361 ttaacagtcc gccgttctac cgactgaact acagaggaat cgtgagaacg aggcgaatat
      421 tagcgatgcc cacccacaat gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa
      481 taatctgcat tttgtcgttt attccgacac aactggcttt ttttcacact tttgcggctc
      541 ggatcgaggg tatttccaca gccaacgtcc agtaaccatt cgccagtaaa acagcacggc
      601 ccgcacagcc cagtcggcaa acattcccat ccagacacca accacacccc aaccaagcat
      661 gattcccagc acataaccga ctacaacccg gcaaccccac atgctcaaca tagaaaccca
      721 catggcgtaa cgggcatcac gagccccctt aaatccagcg ggtagcaccc atgaggcgga
      781 ccaaataggc ataaataaag catttagcca aatcagaatc acaacgacgt gtttaacctg
      841 tggctcctgg gtgtaaaacg atgccataac cccggcaaag ggagccgtta gccaggcgat
      901 ggccgttaat ccaagagtgg acagccagaa cacatgccgc aactgaatct ctgcttgcgc
      961 tatctgccct actcccaacc ttcggcctgt aatgatcgta gaggcagaac cgagcgcact
     1021 tccgggtaag ttgataagag ccgcaattga gaacgcgata aaatttccgg caataacgct
     1081 ggtccccatc ccggcaacga acatttgagt taataaccga ccactggtaa ataacactga
     1141 ttcgacactc gctggaatac caatccccat gacttcccag ataatgctaa aattcagcgg
     1201 tttaaaatag ctctttaacg aaatccttag cgcaggatta aaaccaatcg ccagcaccca
     1261 caaaattgca actgcgccaa tataacgaga aatggttaaa cccaacccag ccccgacaaa
     1321 tcccagtccc ggccaggaga aaaggccgta aatcaatatg ccgctaataa taatattaag
     1381 aatattcagg ctaccgttaa tcagtagcgg tattttcgta ttccctgcac cacgaagtgc
     1441 cccgctacca ataagagtga tggcagctgc tggataactg agtacggtca gctccagata
     1501 agtcaacgcc agtgctttaa cttctgtcgt ggcatcaccc gcgacgaaat caataatttg
     1561 ttcgccaaaa tgatgaatta gcgttgccaa cagtacggca aacaacgtca tgatcaccaa
     1621 tgactgccgc gtcgccaccc tcgctcgtcg tcgatctcgc ttaccgagac taaatgctac
     1681 aacgacagta gtaccaagat cgatagctgc aaaaaaagcc ataatgacca tattgaagct
     1741 gtccgccaac cccacgccgg ccatcgcatc ttttcccagc cagctgacca gaaaagtgct
     1801 caggaccccc atcaacagga cacaggcgtt ctccatgaag ataggaacag caagcggggt
     1861 tatctcgcgc cagaacaaca ctttgtagct cttgcgttta gagtgccagc gagtgccgtg
     1921 aacaacctgg cgtaaagcag aggagatatt caaagccgac cttaattgca gaaagtgaaa
     1981 ccacatttca aatgatgagg gagaatcagc aaagctgcaa agattttcgc caacaaattg
     2041 tctgcaaatg caacaaactg ttgatagaaa cggcaaacag ttgggaaatt taaaaatcgg
     2101 gtttgacaaa atatttttcg ccgttaagat gtgcctcaac agcgattcct ctgtagttca
     2161 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     2221 caatttcctg ctttcatgca tccttgcgaa tccttatgtt atttttattc aacaggttag
     2281 cgtgaaaact cttcccggtg cattttgatt ttaccctctg catcgggaaa aattggtggt
     2341 caaatctggg gtcaggttag ttcgataatg gagtgacccc catatgtccc ttaacgacgc
     2401 aaaaatccgt agtctcaagc ccactgataa accctttaaa gtctccgatt cccacggtct
     2461 gtatctgctg gtcaaaccag gcggttcccg tctctggtat cttaaatacc gtattaatgg
     2521 caaagaatcc cgtattgcac tgggtgccta ccctgccgtc tccctgtctg acgcccgcca
     2581 gcagcgcgaa ggcatccgta agatgctggc gctgaacatc aacccggcgc agcaacgtgc
     2641 cgctgaacgc ggctcgcgca tgcaggagaa aatgtttaaa tccgtggcac tggagtggca
     2701 cagtagcaag aaaaaatggt cgcagaatac cgcagatcgc gtgcttgccc gtctgaacag
     2761 acatgtcttt ccgaccatcg ggcatctacc agttaccgag cttaaatcac gtcatttcat
     2821 tgaattactg aaaggcattg aggaaaaggg cttgcttgaa gttgcgtccc gctcgcggca
     2881 acacctcagc aacattatgc gttatgctgt tcatcagggg ttaatcgaaa tcaacccagc
     2941 tgcaaatctt gacggcgtga cggcttctcc cgccagacga cattatccca ccctgccact
     3001 ggagcgcctt cctgagctgc ttgaacggat tgacagttat caccaaggac gggaactcac
     3061 caggcttgca gtcctgctga cgttgcatgt gttcattcgc tcaagtgaac tgcgctatgc
     3121 tcgttggaca gagattaact tcagaaatcg gatctggacg atacctgcca cacgcgaagc
     3181 gattgcaggc gtacgttatt ccagtcgagg tgccaaaatg cgtacaccgc atattgtgcc
     3241 cctttctgag caggttatct ctattctgaa aaggattaag gaaatatctg gcgggtatga
     3301 actggtgttc cccggctatc atgaccccta caagccgatg agtgaaaata ctatcaataa
     3361 ggcacttcgc ca
//
LOCUS       DADTFG010000251         3358 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.253, whole genome shotgun sequence.
ACCESSION   DADTFG010000251 DADTFG010000000
VERSION     DADTFG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.253"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            362..1678
                     /gene="shiA"
                     /locus_tag="J1138_004544"
     CDS             362..1678
                     /gene="shiA"
                     /locus_tag="J1138_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBA8456224.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1780..3234
                     /gene="amn"
                     /locus_tag="J1138_004545"
     CDS             1780..3234
                     /gene="amn"
                     /locus_tag="J1138_004545"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBA8456225.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          782 a          813 c          807 g          956 t
ORIGIN      
        1 tcgtagtaac taaatgcaat acgtctcaac gatgatacta aaaacataac taacctcgcc
       61 tccctactgg ttataatgca atgcagtcta tcagaatcat cagggtgcaa ttttgtgcat
      121 atgcggactt ttatgtttca tatctcgaac ctgtgggccc tctgcttaat ccttaaacaa
      181 caccagcaac tcctgcgctt tcatcttcca tcgaattttt catgttgccg ctaatcagcc
      241 ataaaatcat ttgcagatgc gctctgtcga ggtagtctca taaggttcgt ttatagatcg
      301 acggcaatgt gagttacctt ttccatacta attataaaaa gacagtacaa acaggatcat
      361 tatggactcc acgctcatct ccactcgtcc cgatgaaggg acgctttcgt taagtcgcgc
      421 ccgacgagct gcgttaggca gtttcgctgg tgccgtcgtc gactggtatg attttttact
      481 ctatggcatt accgccgcac tggtgtttaa tcgcgagttt ttcccgcaag taagcccggc
      541 gatgggaacg ctcgccgcat ttgccacctt tggtgtcggc ttccttttcc gaccgctcgg
      601 cggtgtcatt ttcggtcact ttggcgaccg actgggacgt aagcgcatgt taatgctgac
      661 cgtctggatg atgggcattg cgacagcctt gattggtatt cttccttcat tctcgaccat
      721 tgggtggtgg gcacctattt tgctggtgac actgcgtgcc attcagggat ttgccgtcgg
      781 cggcgaatgg ggaggcgcag cgttgctttc cgttgaaagt gcgccgaaaa ataaaaaagc
      841 cttttacagt agcggtgtac aagttggcta cggagtaggt ttactgcttt caaccggact
      901 ggtttcattg atcagtatga tgacgactga cgaacagttt ttaagctggg gctggcgcat
      961 tcctttcctg tttagcatcg tactggtact gggagcattg tgggtgcgca atggcatgga
     1021 ggagtccgcg gaatttgaac aacagcaaca taatcaagcg gccgcgaaaa aacgcatccc
     1081 ggttatcgaa gctctgttac gacatcccgg tgctttcctg aagattattg cactacgact
     1141 gtgcgagttg ctgacgatgt acattgttac tgcctttgca cttaattatt caacccagaa
     1201 tatggggtta ccgcgcgaac ttttccttaa tattggtttg ctggtaggtg gattaagctg
     1261 cctgacaatt ccctgttttg cctggcttgc cgatcgtttt ggtcggcgca gggtttatat
     1321 cacaggcgcg ttgatcggaa cgttgagcgc atttcctttc tttatggcgc ttgaagcaca
     1381 atctattttc tggatagttt tcttctccat aatgctggca aacattgcgc atgacatggt
     1441 ggtgtgtgtg caacaaccga tgtttaccga aatgtttggt gccagttatc gctacagtgg
     1501 tgctggagtc ggttatcagg ttgccagtgt ggttggcggt ggatttacac cttttattgc
     1561 cgctgcactc atcacttact ttgccgggaa ctggcatagc gtcgccattt atttgctggc
     1621 tggatgcctg atttctgcaa tgaccgcttt attgatgaaa gacaatcaac gctcgtgata
     1681 gcctggcgaa gatcatccga tcttcgcctt atacttttgt ttcacattcc tgtgacatac
     1741 tatcggatgt gcggtaattg tatggaacag gagacacaca tgaataataa gggctccggt
     1801 ctgaccccag ctcaggcact ggataaactc gacgcgctgt atgagcaatc tgtagttgct
     1861 ttacgcaacg ccattggcaa atatattaca agtggcgaat tacctgatga aaacgcccgc
     1921 aaacaaggtc tttttgtcta tccatcactg accgtaacct gggacggtag cacaactaat
     1981 ccccccaaaa cgcgcgcatt tggtcgtttt actcacgcag gcagctacac caccacgatt
     2041 actcgcccta ctctctttcg ttcgtatctc aatgaacaac ttacgttgct gtatcaggat
     2101 tatggtgcgc atatctctgt gcaaccctcg cagcatgaaa tcccttatcc ttatgtcatt
     2161 gatggctctg aattgacact tgatcgctca atgagcgctg gtttaactcg ctacttcccg
     2221 acaacagaac tggcgcaaat cggcgatgaa actgcagacg gcatttatca tccaacggaa
     2281 ttctccccgt tatcgcattt tgatgctcgc cgggttgatt tttccctcgc acggttgcgc
     2341 cattataccg gtacgccagt agaacatttt caaccgttcg tcttgtttac caactacaca
     2401 cgttatgtgg atgaattcgt tcgttgggga tgcagccaga tcctcgatcc tgatagtccc
     2461 tacattgccc tttcttgtgc tggcgggaac tggatcactg ccgaaaccga agcgccagaa
     2521 gaagccattt ccgaccttgc atggaaaaaa caccagatgc cagcatggca tttaattacc
     2581 gctgatggtc agggtattac tctggtgaat attggcgtgg gaccgtccaa tgctaaaacc
     2641 atctgcgatc atctggcagt gctacgcccg gatgtctggt tgatgattgg tcactgtggc
     2701 ggattacgtg aaagccaggc cattggagat tatgtccttg cacacgctta tttacgcgat
     2761 gaccacgttc ttgatgcagt tctcccgccc gatattccta ttccgagcat tgcggaagtg
     2821 caacgtgcgc tttatgacgc taccaagtta gtcagcggca ggcctggtga agaagtcaaa
     2881 cagcggttac gtactggcac ggtggtaacc acagatgaca ggaactggga gttacgttac
     2941 tcagcttctg cacttcgttt taacttaagc cgggccgtag caattgatat ggaaagtgca
     3001 accattgccg cgcaaggata tcgtttccgc gtgccatacg ggacactact gtgtgtttca
     3061 gataaaccgt tacatggtga gattaagctt cccggccagg ctaaccgttt ttatgaaggc
     3121 gctatttccg aacacctgca aattggcatt cgggcgatcg atttgctgcg cgcagaaggc
     3181 gaccgactgc attcacgtaa attacgaacc tttaatgagc cgccgttccg ataataagtt
     3241 atgaaccttc ttatcagaaa tagatgtgaa ggattaagta agacatctgg caaatagcct
     3301 gcaataaacg cgggctgttt gcttccttgg gcagatgcga gttttattat cgtcttaa
//
LOCUS       DADTFG010000252         3349 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.254, whole genome shotgun sequence.
ACCESSION   DADTFG010000252 DADTFG010000000
VERSION     DADTFG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.254"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            193..705
                     /locus_tag="J1138_004546"
     CDS             193..705
                     /locus_tag="J1138_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456226.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTAFYRQVQEHITERNHTASPAPPVVLRWLREQHGGATIRCLLLLSQASICH
                     LRVSATVDEECSQVVDLLQQAWRGINAGGQCRVERCFRVTRPDTSEQYVALKTAVQSL
                     MPLVIATIIR"
     gene            947..1519
                     /locus_tag="J1138_004547"
     CDS             947..1519
                     /locus_tag="J1138_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456227.1"
                     /translation="MDYVTSFELPFRLLLTRTPQLIVTLREAWDISQKNVVFNDKRFG
                     CVYSLKASLSGVPDIFRYHLSHRIRRMVGSENTSSPWQQIAREVKAPRERLKYALKTG
                     LQVIALNGLFWSDSQRLVVDILRLSANRRDTGNRPHPHLPSYITTGADTESMVVRDSV
                     WQPREQRPEVIDRSLAPGYVCSPRTDRLSL"
     gene            complement(1784..2997)
                     /locus_tag="J1138_004548"
     CDS             complement(join(1784..2703,2703..2997))
                     /locus_tag="J1138_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA8456228.1"
                     /translation="MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     ELMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEQNTIFKKG
                     YCTLDVRLAERFTIVARLSGSHTVVSLCSALGIHRSSYRYWQKRRDTVNPVHVRLCSE
                     ILRVWNQSRGSAGARTLADMLTQNGVPMSRYRAGRLMKHLNLSSCQPGKHQYKNARQE
                     HTCLPNLLGRQFAVPEPDQVWCGDITYIWAGNCWCYLAVVMDLFARRVIGWSLSAHAD
                     TALISDALRMAYETRGQPHDVMFHSDQGSQYTGLKYQQILWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPANGYVDKDEARQQINDYILNYYNSVRPHHYNGGLTPEESGNR
                     YHFYCKTVASIT"
     gene            complement(3055..3339)
                     /locus_tag="J1138_004549"
     CDS             complement(3055..3339)
                     /locus_tag="J1138_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial major subunit operon
                     transcriptional regulator"
                     /protein_id="HBA8456229.1"
                     /translation="MNCQKQVSHLLSFCLYREDDDMQNEIMGFLSRHNGGKTAEIAEA
                     LAVTDYQARYYLLLLEKEGMVQRSPLRRGMATYWFLKGEMQAGQSCSSTT"
BASE COUNT          798 a          787 c          832 g          932 t
ORIGIN      
        1 atataagtat tggtggtatt gtcagtaata ttcttttggt taatttagtt attattcctc
       61 actgattaat tgtccttccg ttcttattgt ttgtaacttg tgtttgtatc gtacctttca
      121 ggtgttgatg cgcgctttac tgtgtctgca ccggcttgac aaaattcaac agggtttgaa
      181 aaggaacatt tcgtgcaaat aaccgaagcc ttaatttcag agccgggaga catccggcgt
      241 tttgttcagc aggcggtgga ccactggcca aatctgttgg catttcactt cacgctctat
      301 tcggcagaag gaaacatcaa cgggcaacag attcatgcat tctgtactgc cttttaccga
      361 caggtgcaag aacatattac tgagcgtaat cacactgcca gtccagcacc gccggtggta
      421 ttacgctggt tgcgggaaca acatggagga gcaacaatac gatgcctgtt gctgctcagc
      481 caggcgagca tttgccatct gcgggtcagt gccacagttg atgaagaatg ttcgcaagtg
      541 gtggatttac tgcagcaggc ctggcggggg ataaacgctg gcggacaatg tcgggtggaa
      601 aggtgttttc gggttacccg gcctgataca tcagagcaat acgttgcgtt aaaaacagct
      661 gttcagtctc tgatgccact ggttattgca acaattatcc ggtgatgatc gggagaaata
      721 tcaactcttc cgttaatttt tacagactga taaagttttg tctgctgaat ctttatacgg
      781 aattcttttc agtatgtgta cacgaattga ctctatggcg gataaatact gttttacctt
      841 ggcggaatta atcctcacgt cttcgccagc agtggcagga tgtcctggag gagtgtcggc
      901 agactgaggc ggggcagaaa aacggctgca tattgcactg ctgaatgtgg attacgtcac
      961 cagttttgaa ctgccttttc gtctgctttt aacccggacg ccacagctga ttgttacact
     1021 tcgggaagca tgggatatca gccagaaaaa tgtggtgttc aacgataaac ggtttggctg
     1081 cgtgtacagc ctgaaagcca gtctttctgg tgtgccggat atattccggt atcatctgtc
     1141 tcatcgtatt cgccggatgg ttgggagtga aaatacatca tcgccatggc agcaaattgc
     1201 ccgggaagtg aaagcgcccc gtgaacgact gaagtatgcg ctgaaaacgg gattgcaggt
     1261 gatcgcactg aacggcctgt tttggtcaga cagtcagcgt ctggtggtgg atattctgag
     1321 gctaagcgcc aacagacgtg atacaggaaa tcgtccgcat ccccatttac cgtcttatat
     1381 cactactggc gctgacactg aaagcatggt cgtcagagac tctgtctggc aaccaaggga
     1441 gcagaggccg gaggtaattg acaggagcct tgcgccgggt tacgtatgtt ctccccggac
     1501 agacaggctc agtttgtaga atctacaaat tactactact gatgctgccg gggaaaggcg
     1561 taacgaaaaa acagcctccg ttaccggata gcaaggaggc tgaatggagt ttataggatt
     1621 tgcgttttta taatgtctgg ccatgcggta aaaccggaca ggttttatta tcatgtgagg
     1681 tattctgaca taaaatgctg gatttttatt ttgtgacgaa tgctgcaaaa ttgaatctgc
     1741 actctgatgt aacttttatc tttttcagtg aagtatgtag tggtcaagta atactggcca
     1801 cggttttaca gtaaaaatgg tacctgttcc cggactcttc cggtgtcaac ccaccgttat
     1861 aatggtgagg tctgacactg ttgtagtagt tcaatatgta atcattaatt tgctgccggg
     1921 cctcgtcctt gtcgacgtaa ccattcgctg gcacccattc tgttttcaga ctgcggaaga
     1981 agcgttccat tgggctatta tcccagcagt ttccccgccg actgacactt tgctttattc
     2041 tgtaacgcca gagaatttgt tgatatttca ggcctgtata ctggcttccc tggtcgctat
     2101 ggaacataac atcatgtggt tgaccacgcg tctcataggc catccgcagg gcatcgctta
     2161 tcagtgcagt atcggcatgt gcggacagac tccagccgat aaccctgcgg gcaaaaagat
     2221 ccatgacgac cgccagatag caccagcaat ttccggccca gatataggta atatctccac
     2281 accatacctg gtctggctcg gggacagcga actggcgccc gagcagattc ggcaagcagg
     2341 tatgctcctg acgggcattt ttgtactgat gttttccggg ctgacaactg ctcagattca
     2401 gatgtttcat cagccgcccg gcacggtaac ggctcatcgg gacgccgttt tgggtcagca
     2461 tgtcagccag cgtgcgcgcc cccgcagagc cccggctttg gttccacacc cggagtattt
     2521 cactgcacaa cctgacatgc accggattca ccgtatcgcg tcgtttttgc cagtaccggt
     2581 aactgctgcg gtgtattccc agagcagaac acagactgac aacggtgtgg ctgccactga
     2641 gtctggcaac tatcgtgaac cgttcagcga gtcggacatc aagagtgcag tagccttttt
     2701 taatatcgta ttttgttcct ccaggcggcg aacctgcttt tccagctcgc ggatacgttg
     2761 ctggtctgga gtaatcggtg tggcagaggg ggcaatcccc tggcgctctc gtctgagctg
     2821 acgtacccag ctctcaagag tggtagagcc gacattcatc aattcactgg cttgtcgata
     2881 tgagtagccc ttatcaacaa tcagttgcgc acattccagt ctgaactcgg gggtgaaagt
     2941 acgtttggtt ttcttgttca ttaagtcacc tgttttgtgt tgaggtgaga atatcacctt
     3001 taatcaggtg gccaaactta ctgtgccact acagtatgcc cacagattga gttattaagt
     3061 tgtggaagaa cagctctgtc ccgcctgcat ctctcctttc agaaaccagt atgttgccat
     3121 gcctcgcctt aatggagagc gctgaaccat accttctttt tccagtaata acaggtaata
     3181 gcgtgcctga taatccgtta ccgccagcgc ctccgcaatt tctgcggttt tccctccatt
     3241 atgtctgctc agaaaaccca ttatttcatt ctgcatatca tcatcctctc tatataagca
     3301 aaaggataat aagtggctga cttgtttttg acaattcaaa tgtgattat
//
LOCUS       DADTFG010000253         3345 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.255, whole genome shotgun sequence.
ACCESSION   DADTFG010000253 DADTFG010000000
VERSION     DADTFG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.255"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            208..1728
                     /locus_tag="J1138_004550"
     CDS             208..1728
                     /locus_tag="J1138_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBA8456230.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1753..2091)
                     /gene="maoP"
                     /locus_tag="J1138_004551"
     CDS             complement(1753..2091)
                     /gene="maoP"
                     /locus_tag="J1138_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBA8456231.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2210..3049
                     /gene="hdfR"
                     /locus_tag="J1138_004552"
     CDS             2210..3049
                     /gene="hdfR"
                     /locus_tag="J1138_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBA8456232.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3145..3220)
                     /locus_tag="J1138_004553"
     tRNA            complement(3145..3220)
                     /locus_tag="J1138_004553"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3185..3187),aa:Trp,seq:cca)
     gene            complement(3229..3305)
                     /locus_tag="J1138_004554"
     tRNA            complement(3229..3305)
                     /locus_tag="J1138_004554"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3269..3271),aa:Asp,seq:gtc)
BASE COUNT          901 a          824 c          813 g          807 t
ORIGIN      
        1 agtgaaaaaa attcaatttc agtatgaatg ttgttccctt cctcgtagtt catcctttgc
       61 acgctgcatt tttgcgagca tcattccatc gccagaacaa ctcaacttca cttgcacaaa
      121 taaacaggaa tcagccacta aaattcacga attagcgggc tacagcagcg atatcttatg
      181 cacataatgg tgactcaagg agggcttatg tcactgtcaa ttgttcatac ccgcgcagcc
      241 ctgggagtaa atgcgccccc aatcactgtt gaggtacata tcagtaaagg tctaccaggc
      301 ttaacgatgg tgggcttacc agaaacaacg gtaaaagaag ctcgcgatcg cgtgcgcagc
      361 gccattatca atagcggata tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca
      421 gccgatctgc caaaagaagg gggacgatat gatttgccta tcgctattgc gttactggcg
      481 gcctcagaac agcttacagc caataagtta gatgaatatg aattagtcgg agaactggcg
      541 cttacaggcg ctctgcgtgg cgttcccggc gcaatctcca gtgcaactga agctattaag
      601 tcgggcagaa aaattatcgt cgcgaaagat aacgaagatg aagtggggct aattaacggt
      661 gaaggatgcc tgatagccga tcatctgcag gctgtctgcg cgtttctgga aggtaagcac
      721 gctctcgaac acccgaaacc aactaatgca gtatcccggg cgctacaaca tgatctcagt
      781 gatgttgtcg gtcaggagca aggaaagcga ggactggaaa ttaccgccgc tggcgggcac
      841 aaccttttac tgattggacc gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat
      901 ggccttttgc cagatttaag caatgaagag gcactggaga gcgctgcgat attaagtctg
      961 gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc cgttccgctc acctcatcac
     1021 agtgcgtcgt taactgcgat ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg
     1081 ctggcgcata acggcgtgct ttttcttgat gagctacctg aatttgaacg gcgtacactg
     1141 gatgccttgc gagagccgat tgaatccggg cagatccatc tttcacgcac acgagcaaaa
     1201 ataacctatc cagcccgttt ccagcttgtc gcggcaatga atcccagccc taccggacat
     1261 tatcagggaa accataaccg ctgcacacca gaacagacat tacgttatct caaccggctc
     1321 tcggggccct ttctcgaccg cttcgatctc tcactggaga tcccattacc gccccccggc
     1381 attttgagta aaacggtagt gccgggagaa aacagcacca ccgttaaaca acgtgtaatg
     1441 gccgccagag agcgccaatt taagcggcag aataaactga acgcctggct ggataatccg
     1501 gaaatacgcc aattctgcaa gcttgaaagc gaagatgcgc agtggctgga agaaacgctg
     1561 atccatctgg ggttatcgat tcgtgcctgg cagcggttat tgaaagttgc acgaaccatt
     1621 gctgatattg atcagtctga cattatcaca cgtcagcatt tgcaggaggc agttagctat
     1681 cgtgcgattg accgtttgct catccatctg caaaaactac tgacataaaa aaagggcatt
     1741 tcgccctttt tattaatcgt cagaatcggt gtagtcttca gcaccttcaa cctgcggttt
     1801 accgccggaa agggtgtgaa aacgttttgg acgcttgata cgcgtcatat acttggacca
     1861 cacgcgttct gcttctgtca ctggttcacg ttcgccacgg catactgcta cgaagagttt
     1921 ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac tcattgaagg cataaccatg
     1981 acgctcaagc agttgtgcct ctttgatggt gaagtcacca tgacgagaga atccacgtgg
     2041 ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc
     2101 tcctaattct ttgaccgagc tagttatggc gcggagtatt agttacgctt gacagagtgt
     2161 aaaacaaaac atttaaatca taacgacaaa taattttgcg gagagcactg tggatacgga
     2221 attgttaaaa actttcctgg aagttagccg aacgcgtcac tttggtcgag cggctgaatc
     2281 gctctatctg acccaatcag ccgtgagctt tcgaatcaga caactggaaa atcaactggg
     2341 tgtgaatctt ttcacccgcc acagaaacaa tatccgttta accgctgccg gagaaaagct
     2401 tctgccttat gcagaaacgc tcatgagcac ctggcaggcc gcccgcaagg aggtggcgca
     2461 tacctcacga cataatgagt tttctatcgg tgccagcgcc tcgctgtggg agtgtatgct
     2521 taatcagtgg ctgggacgct tgtatcaaaa tcaggatgtc catacaggct tacagttcga
     2581 agcgcgaatt gcccaacggc agtctctggt aaaacagttg catgaacgcc agcttgatct
     2641 tcttattaca actgaagccc ccaaaatgga cgaattttgt agtcagttgc tggggtattt
     2701 cactttagcg ctttatacca gtgccccttc taaactaaag ggagatctta actatctgcg
     2761 actagagtgg gggccagact ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt
     2821 tcccattctg acaaccagtt ctgctgaact ggcacagcaa cagattgcga tgcttaatgg
     2881 ttgcacctgg ctacccgtca gttgggcacg taaaaaaggc ggcctgcata ccgttgtcga
     2941 tagcacaaca ctttcacggc cgctttatgc catatggcta caaaatagcg ataaaaatgc
     3001 gttgattcgc gatctattga aaattaacgt gctggatgaa gtgtattaat ctgaatggct
     3061 ggcaaggatg ccggtagaag gatttacttc ggagagggtt atttcagata aaaaaaatcc
     3121 ttagctttcg ctaaggatga tttctggcag gggcggagag actcgaactc ccaacacccg
     3181 gttttggaga ccggtgctct accaattgaa ctacgcccct aattagggtg gcggaacgga
     3241 cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc
     3301 gctccaccga attcttttac aaccaccggt ttatgaccgg cttac
//
LOCUS       DADTFG010000254         3342 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.256, whole genome shotgun sequence.
ACCESSION   DADTFG010000254 DADTFG010000000
VERSION     DADTFG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.256"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            19..516
                     /gene="ftnA"
                     /locus_tag="J1138_004555"
     CDS             19..516
                     /gene="ftnA"
                     /locus_tag="J1138_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBA8456233.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(554..793)
                     /locus_tag="J1138_004556"
     CDS             complement(554..793)
                     /locus_tag="J1138_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBA8456234.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            984..2195
                     /gene="tyrP"
                     /locus_tag="J1138_004557"
     CDS             984..2195
                     /gene="tyrP"
                     /locus_tag="J1138_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBA8456235.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2257..2922)
                     /gene="yecA"
                     /locus_tag="J1138_004558"
     CDS             complement(2257..2922)
                     /gene="yecA"
                     /locus_tag="J1138_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBA8456236.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3118..3204)
                     /locus_tag="J1138_004559"
     tRNA            complement(3118..3204)
                     /locus_tag="J1138_004559"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3168..3170),aa:Leu,seq:taa)
     gene            complement(3217..3290)
                     /locus_tag="J1138_004560"
     tRNA            complement(3217..3290)
                     /locus_tag="J1138_004560"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3256..3258),aa:Cys,seq:gca)
BASE COUNT          771 a          847 c          800 g          924 t
ORIGIN      
        1 cctttgtgga gcactatcat gctgaaacca gaaatgattg aaaaacttaa tgagcagatg
       61 aacctggaac tgtactcttc actgctttat cagcaaatga gcgcctggtg cagctatcat
      121 accttcgaag gtgctgccgc gttcctgcgc cgtcacgccc aggaagagat gacgcatatg
      181 cagcgtctgt ttgattacct gactgatacc ggcaacttac cgcgtattaa taccgttgaa
      241 tctccgtttg ctgaatattc ctcacttgat gaattattcc aggaaaccta taaacacgaa
      301 caattaatca cgcagaaaat taacgaactg gctcatgctg caatgaccaa tcaggactac
      361 ccaacattta atttcctgca atggtatgtt tctgagcagc atgaagaaga gaaactgttc
      421 aaatcgatta ttgataaatt aagccttgca ggcaaaagcg gcgaaggtct gtattttatc
      481 gacaaagaac tctctaccct cgacacacaa aactaatgct ctccggcggc aggttttctg
      541 ccgccgtttc aaatcagtga cggcaaatct tactctgatc ggttgaaaaa ccttcttctg
      601 aaggtataaa tttgcctttt gctgccagaa acgccaccaa ctctcccgca gtcatccctt
      661 ctgccgagca ggtgtgaaaa cgtgcctgtt caccaaaacg cgctttaatt gctgcttcca
      721 gactggcatg cgtatattgc tcgcctgatt caatcatcat atttaacact tcgtgaccgt
      781 gaatagagtc catcgttcct cctcaaaaaa agcctagcgt agcgattgcc gcttatgaag
      841 actttgcgcc agcgcagaac tgaacgcttt ttattgtaca tttatattta caacatatgt
      901 aacgtcggtt tgacgaatca gccgttatgc cttaacctgc gccgcagata tcactcataa
      961 agatcgtcag gacagaagaa agcgtgaaaa acagaactct gggaagtgtt tttatcgtgg
     1021 cgggaaccac aattggcgct ggtatgctgg caatgccgct ggctgcggca ggtgttggtt
     1081 ttagcgtcac gttaatcttg ttaattgggc tttgggcatt gatgtgctac acggcgctat
     1141 tactgctgga ggtgtaccag catgtcccgg cagataccgg tctgggcacg ctggcaaaac
     1201 gctatctggg acgctacggt cagtggctga cgggcttcag tatgatgttc ttaatgtatg
     1261 ctctgactgc ggcatacatc agcggtgccg gtgaattgtt ggcctccagc atcagcgact
     1321 ggacaggtat ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg
     1381 gcgtggtttg tgtcggaact tcgctggtcg atttatttaa ccgttttctg ttcagcgcca
     1441 aaattatttt tttggtggtg atgctggtat tgctgctacc gcatattcac aaagtgaatc
     1501 ttttaaccct gccgttgcaa cagggactgg cactgtctgc aatcccggtg atttttacgt
     1561 cgtttggttt tcacggtagc gtgccgagta ttgtcagcta tatggatggc aacattcgta
     1621 agctacgctg ggtgtttata atcggtagtg cgatccccct ggtggcatat attttctggc
     1681 agctggcgac ccttggcagc attgattcaa caacctttat gggattgctg gctaatcatg
     1741 ccggattaaa cgggctgtta caggcgttac gcgaaatggt ggcctctccg catgttgagc
     1801 tggcagtgca tttatttgct gatttagccc tcgccacgtc cttcctcggc gtagcgttag
     1861 gcttatttga ttatctggca gatttgtttc agcgttcaaa taccgttggt ggacgattgc
     1921 aaactggtgc aattaccttt ctgccgccac tggcgtttgc actgttttat ccacgaggat
     1981 ttgtgatggc gctgggttac gccggtgtgg cactggcggt actggcattg attatccctt
     2041 cgctgttgac ctggcaaagc aggaagcaca atcctcaggc gggttaccgg gtgaaaggtg
     2101 gtcgtccggc gctggtggtg gtgtttctct gtggtattgc tgtgattggc gtgcaatttt
     2161 tgattgcggc aggcttgtta ccagaagtgg gatgattaga tagcctcaaa ttccttattg
     2221 ggtgccagaa ttaacgctga cacccaatgt gacctcttaa tgcaggcagc actgcttaaa
     2281 tttcttacca ctaccgcacg gacaaggatc gttacgcccc ggtttctctt ctgctttgat
     2341 cggttgctga acagcttttt cctgcggatg cgccatccag tatgcatgta aatcaagcgc
     2401 cgccagtcga atggcatcta cgctctcttc aaacgcttct ggcgacatct tctctacccg
     2461 ctcgaagttt tcctcagtac cgtgcagcgc aatcgcctcc agcgctggtt ttaacgaatc
     2521 gggcaacgtt gaccagtcag aaagtgccac gccccgcata tagccaaagc accactcctc
     2581 aacaatcgtc agctcgctgc catcgacttc tcgcaagccg aataacggct caaactgctc
     2641 cgggaattcg ttcagacgct ctgcggtatc ggccatatgt tgaaaagcca ggttcataaa
     2701 gcgcgtcatc tctttctctg acgcccagcg cggcacatag tcagccccac cccacacggc
     2761 aaccagccac tgtgccggtt caatctcttg cggagaactc aacaccgccg tcaataaacc
     2821 gtctagctcc gccacatcaa gaatggcgtg gtcagtgttg tatttggtca gaatatcgtc
     2881 cagccattcc aactcacttt cgtttaacgg tcccgttttc atacgctttt ccttgtggat
     2941 ctcaactcgc cagcacctat cttacatgcc ggtccgtatc agagatactt tttgagtggc
     3001 tttgccggtg attaaaaatt aaggagggtg taacgacaag ttgcaggcac aaaaaaacca
     3061 cccgaaggtg gtttcacgac actgcttatt gctttgattt tattcttatc tttcccatgg
     3121 tacccggagc gggacttgaa cccgcacagc gcgaacgccg agggatttta aatcccttgt
     3181 gtctaccgat tccaccatcc gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac
     3241 cggactagac ggatttgcaa tccgctacat aaccgctttg ttaacgcgcc aaattcttca
     3301 ggcctttcag ccagacatcc gctgccgccg atgtctttta aa
//
LOCUS       DADTFG010000255         3292 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.257, whole genome shotgun sequence.
ACCESSION   DADTFG010000255 DADTFG010000000
VERSION     DADTFG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.257"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            46..1596
                     /locus_tag="J1138_004561"
     CDS             46..1596
                     /locus_tag="J1138_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA8456237.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKILA
                     VDKNRLIGGMANIAGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQAVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGMRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1637..3256
                     /locus_tag="J1138_004562"
     CDS             1637..3256
                     /locus_tag="J1138_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA8456238.1"
                     /translation="MKTAQRTLLAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSNVVIDYFKKGHKITPAQVNEMRVEVSK"
BASE COUNT         1031 a          686 c          705 g          870 t
ORIGIN      
        1 tttaaataat tcaacaaatg taacaacccc tatccaggag ggatgatgaa aactgtattt
       61 acaacattat cagttatatt cggcgtgata ttttctcaca gtgttctagc tcaggacgtt
      121 actatttatt atacgaatga cattcacgca catgtcaatc cggcaaaaat cctcgctgtt
      181 gataagaaca gacttatagg tggtatggct aatattgcag gcatcgttaa tgaagcgaag
      241 aaaaaaagca aagatgtgtt tttctttgat gcaggcgact attttaccgg gccgtatatc
      301 agcacactga ccaaaggtga agctattatt gatatcatga acactatgcc ctttgatgcg
      361 gtgtctgttg gtaatcacga gttcgatcac ggtgtgccca atatggtgtc tcagttatca
      421 aaagcaaaat tccccattct gctgggaaat atctactaca ccgatacgaa taaaccagtt
      481 tgggatcatc cgtggaccat tatcgaaaaa gatggtttaa agattggtgt gattggattg
      541 catggcgcat tcgctttcta tgatacagtc gcggcaaaag cacgtgaagg cgtagaagcc
      601 agagacgaaa ttaaatattt aaataaagca cttgcagaat taaaaggtaa agtcgacata
      661 accgttttgc taattcacga aggtgtcccg gcgcgtcaat caagctttgg tagtaaagat
      721 gtggagcggc tactacaggc agatattgag acagctaaaa aagtgaatgg tgttgatgtc
      781 ttaattaccg gtcacgcaca tgtcggaaca ccgcaaccaa taaaagttaa taacacatta
      841 attgtatcta ccgatgcata cggcaccaac atcggaaaac ttgtgcttga ttacaatccg
      901 aaaacgaaga aaattgatag ttataatggt gagttaatca ccatctttgc agatcaattt
      961 aaacctgaca ccatcgttca aaatactatt gataaatgga gtgcaaagct taacaaaata
     1021 acacaggcag ttgtcggcca ttctcccgta gttttaacac gtgagtatgg tagttcctct
     1081 tctaccggca accttattct tgatgcaatg atggaaaaaa cacctgatgc cgttgccgga
     1141 tttcaaaata gcggcgggat gcgagctgat tttcctaaag gcgatatcac actgggagat
     1201 gttattagca cattcccctt taataatgac ctcatcgaga tggatttgac gggccgcgat
     1261 ctcaaatcat tgatgacgca tgcaaccaat ctaactaacg gtgtgttaca ggtttcaaaa
     1321 agcgttgcgg ttgtctatga cagcaaaaaa ccactcaacc aacggttaat ctctttcacc
     1381 attaacggca aacccgtgga agataatcaa acatatcgta ttgccacgca ctccttttgt
     1441 gccagtggtg gtgacggttt tgaagcattt ttgaatggaa aaaatgtgaa gacgataccg
     1501 ggaacaacct cggcggaatc tatcatcgat tatttcaaaa atcataaacc tgtcacccca
     1561 gacttaacta aacgagtcat ggacgtcgcc aaataaaatc tcatttatca gtcaatttat
     1621 ttaacaagga atgcgaatga aaacagcgca gcgtacttta cttgccgcaa cattgctgac
     1681 tattttttcg acctcagtca tggcgcagga tgtcacgatt atttatacca atgatcttca
     1741 cgctcatgtg gattcctata aagtaccgta tgtcgcagac ggaaaacgcg acattggtgg
     1801 ctttgccaat atatccacgt tagtaaaaca agagaaagcc aaaaataaag ccacctttta
     1861 ttttgatgct ggagactatt tcaccgggcc atatatcagt agcctgacaa agggcgaagc
     1921 aataattgat attatgaata ccatgccttt tgacgcagta tcaatcggta atcatgagtt
     1981 tgaccatggc tgggataatg ccctccgaca attaagcaaa gcgaatttcc ctgttttatt
     2041 aggcaatgtt tatcataaag agagtgagaa acccttctgg aacaagccct acaccatcct
     2101 ggaaaaagat ggcatcaaga ttggtattgt tgggctacac ggcgtatttg catttaatga
     2161 cacggtctcg gagctctctc tccagggact cgataatgac aataataacc gatttgataa
     2221 atcttccgcg accctcaaga atcaggggat tgaagcacgt gatgaagtga agtatttgca
     2281 gcattatatc gatgagttac gtgataaggt tgacctcacc gtggcgctgg ttcatgaagg
     2341 cgtaccggcg cgtcagtcca gcattggcaa tacagatgtt agacgtgcgc tggataaaga
     2401 tattcaaacc gcaagcaagg tgaaaggatt ggatattctg attaccgggc atgcccacgt
     2461 cggaacgcca gaaccaatca aagtcggcaa caccttaatc ctttcgactg acagtggcgg
     2521 tatcgatatt ggtaaattaa tccttgatgt caaccctaca gctcgtactc ataaagtgaa
     2581 gagctttgag ctgaaaacgg tttatgccga tgaatggata cctgatccaa ccacacaaaa
     2641 agttatcaat ggctggaata aaaaactggc ggatcttgta cgccaaccag tcggtgaatc
     2701 atcgattgca ttaactcgtg cctacggtga gtcatcgcaa cttggcaacc tgtttaccga
     2761 tgcaatgctt gtcgctgcac cgactgcaca aattgcgttg ataaactccg gcagtttacg
     2821 tgctgatata aatgcaggca cgattacctt tggtgacatt accagtacat tccccttcaa
     2881 gaatgaactt actgaaatgg atctcagcgg taaggatctg cgtaacctac tggaacacgg
     2941 ggcatcgctt accaatggca tcctgcaaat gtcaaaaggc gcagaaatgc gctataccct
     3001 gcagaaacca gtaggtcagc gcattgtatc ttttaaaatt aatggtgaag agatcgttga
     3061 tacgaatatt taccatgttg cgactacgac tttccttgca cttgggggcg atggttttct
     3121 ggcattcaaa gaagggaaaa atgtccaggt ccgcgccgga aataacatgt ccaatgtcgt
     3181 aatcgattat tttaagaaag gccacaagat tactcctgca caggtaaatg aaatgcgggt
     3241 agaagtaagc aaataacaaa aacttataat accattcatt cactggatga ca
//
LOCUS       DADTFG010000256         3254 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.258, whole genome shotgun sequence.
ACCESSION   DADTFG010000256 DADTFG010000000
VERSION     DADTFG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.258"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            25..501
                     /locus_tag="J1138_004563"
     CDS             25..501
                     /locus_tag="J1138_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBA8456239.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            564..785
                     /locus_tag="J1138_004564"
     CDS             564..785
                     /locus_tag="J1138_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBA8456240.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            948..1316
                     /locus_tag="J1138_004565"
     CDS             948..1316
                     /locus_tag="J1138_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBA8456241.1"
                     /translation="MSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            1406..1783
                     /locus_tag="J1138_004566"
     CDS             1406..1783
                     /locus_tag="J1138_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBA8456242.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKQ"
     gene            1780..2268
                     /locus_tag="J1138_004567"
     CDS             1780..2268
                     /locus_tag="J1138_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456243.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDIDGVNLAELINNVAENGYS
                     LRVAEESDQQSTCTPPPFTILAGIRCSTAHITETDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRMVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            2280..2477
                     /locus_tag="J1138_004568"
     CDS             2280..2477
                     /locus_tag="J1138_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBA8456244.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            2574..3143
                     /locus_tag="J1138_004569"
     CDS             2574..3143
                     /locus_tag="J1138_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBA8456245.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     SAHITFKRLELIDRINDIIARHFPSVLSA"
BASE COUNT          821 a          893 c          791 g          749 t
ORIGIN      
        1 atcgactgaa tggaggcagg gacaatgcag cagctttcct ttctgcccgg agaaatgacg
       61 cccggcgagc gcagtctcat tctgcgggct ctgaaaaccc tggaccgcca tcttcatgaa
      121 cccggtgtgg ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg
      181 ggactggagc gtgaagagtt ccgggtgctg tatctgaata accagaatca gctgattgcc
      241 ggtgaaaccc tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt
      301 aaacgcgccc tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca cccgtccggt
      361 gaagtcacac ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc
      421 ctggtggata tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt
      481 gccgaacatg gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct
      541 tctgtcttca gaggtatccc attatgaaaa ttatcactcg tggtgaagcc atgcgtattc
      601 accaacaaca tccgacatcc cgtcttttcc cgttctgtac cggcaaatac cgctggcacg
      661 gcagcactga agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt
      721 ttgctgaacg tcgtaaggac agttttggcc cgtatgtccg gctgatgagc gtcaccctga
      781 actgatgttc cgtcacccgg aaaacgagta cgactggctg agctcagacg ccgctgtgga
      841 tgaggccctg ctcataaacg aatacacctt taccgaaggc ggtctgcgcg ccggctgacc
      901 tgtcatcgtt aaaccattct taaccgattt tctgtgagga ttttatcgtg tcagacgcac
      961 tctctgggac aatgcttccc gacgacaatc acgaccgccc ctggtggggg ctgccctgca
     1021 ccgtgacgcc ctgtttcggg gcacgtctgg tgcaggaggg taaccgcctg cattaccttg
     1081 ccgaccgcgc cggtatcaga ggcctgttca gcgatgcaga tgcgtaccac ccggaccagg
     1141 cctttccgct gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc
     1201 gccatcagca taccgtcacg ctgtatgcaa aaggtctgac ctgcaaagct gacactctcg
     1261 gtagcggtgg ttacgtttat ctggctgttt atccgacgcc cgaaacgaaa aagtaaacca
     1321 ccggaataac cttctgctcc ggcctggtgc tttcaccacg ccacttttcc atttttcatc
     1381 tctgcatatc aggaaaacct tcagtatgaa aacattaccc gatacacacg tacgggaggc
     1441 atcgagctgc ccgtctcccg tcaccatctg gcagactctg ctctcccgac tactgggcca
     1501 gcattacggc ctcacgctga atgacacacc gttcgccgat gaacgtgtga ttgagcagca
     1561 tattgaggcg ggtatttcac tgtgtgacgc ggtgaacttt ctcgttgaaa aatacgcgct
     1621 ggtacgtacc gaccagccgg gattcagcgc ctgtacccac tctcagttaa taaacagcat
     1681 cgatatcctt cgggctcgcc gggcaaccgg cctgatgacc cgcgacaatt acagaacagt
     1741 aaataacatt accctgggta agcatccgga ggcgaaacaa tgaaactggc cctgacgctg
     1801 gaagccgaca gtattaacgt gcaggcgctg aacatggggc gcattgtcgt tgacattgat
     1861 ggtgttaatc tcgctgaact gattaacaat gtcgctgaaa acggttattc actccgcgtg
     1921 gcggaggaat ccgaccaaca gtcaacctgc acaccaccac cgtttacaat ccttgccggc
     1981 atacgctgca gtaccgcaca tatcacggaa acagacaacg cctggctgta ctcgctgtcg
     2041 catcagacca gtgacgttgg tgaatcggaa tggattcatt tcacaggtag cggatatctg
     2101 ttacgtaccg atgcgtggtc atacccggtt ctgcggctta agcgcctggg cctgtcaaaa
     2161 acgttccgtc gtatggttgt cacacttacc cgacgttatg gcgtcagtct cattcatctg
     2221 gatgccagcg ctgaatgcct gccgggttta cccactttca actggtaacc aggaacaaca
     2281 tgaaatcatt aaccacggaa acagcactgg atattctgat tgcgtggctg caggacaata
     2341 tcgactgcga atcgggaatt atctttgaca acaatgagga taaaacggat tcagcagcac
     2401 tgttgccctg tatcgaacag gccagagagg atatccgtac cctgcgccaa ctgcagcttc
     2461 agcaccagaa ccggtgagtc tcactcatca tctcactcac cagacttcat tccactgacg
     2521 ccagcctgaa cacggctggc gttttcattt atctgcaaaa aggaatatcg attatgtctg
     2581 aaatcacagt ctcccgtccg gaagtggtca acgagaatac ggacgttatc tgctccacct
     2641 cagtcaggtc caggtcactg gaatatgata attttccgga aatcagcgaa gcgaacattc
     2701 tgagcacatt tgaacaactg caccagaaca aagatgaagt gtttgaacgg ggagtgatca
     2761 acgtcttcaa agggctgagc tgggattaca aaaccaactc accctgtaaa tttggcagta
     2821 aaattatcgt caacaatctg gtgagatggg accagtgggg atttcatctt atcagtggaa
     2881 tgcaggcaga tcgcctggct gacctggaaa gaatgttgca tctgctcagc ggtaaaccga
     2941 tccccgacaa ccgagggaat atcaccatta atctggatga ccacatacag tccgttcagg
     3001 gtaaaggacg ctatgaagat gagatgttca tcattaaata ctttaagaag ggatctgcac
     3061 acatcacttt caaaaggctg gagctgattg acagaattaa cgatataata gccaggcact
     3121 ttccttctgt gctctcagcc tgaccccgag tttgattccc tttcgatatc aaaagggact
     3181 gcgggtacaa aagagggtac atatttcacc aaaccaaaca aaataaacta atatcaacat
     3241 gatagaagca ttct
//
LOCUS       DADTFG010000257         3212 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.259, whole genome shotgun sequence.
ACCESSION   DADTFG010000257 DADTFG010000000
VERSION     DADTFG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.259"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(49..576)
                     /gene="mobB"
                     /locus_tag="J1138_004570"
     CDS             complement(49..576)
                     /gene="mobB"
                     /locus_tag="J1138_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBA8456246.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(558..1142)
                     /gene="mobA"
                     /locus_tag="J1138_004571"
     CDS             complement(558..1142)
                     /gene="mobA"
                     /locus_tag="J1138_004571"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBA8456247.1"
                     /translation="MNLMTMITGVVLAGGKARRMGGVDKGLLELNGKPLWQYVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1212..1481
                     /locus_tag="J1138_004572"
     CDS             1212..1481
                     /locus_tag="J1138_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBA8456248.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1558..2544
                     /gene="srkA"
                     /locus_tag="J1138_004573"
     CDS             1558..2544
                     /gene="srkA"
                     /locus_tag="J1138_004573"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBA8456249.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPPGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGNKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2561..3187
                     /gene="dsbA"
                     /locus_tag="J1138_004574"
     CDS             2561..3187
                     /gene="dsbA"
                     /locus_tag="J1138_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBA8456250.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          821 a          782 c          776 g          833 t
ORIGIN      
        1 ataaatgccg gatgcgacgc tggcgcgttt gctccagcct actcttgctt atccgtcctg
       61 cttttgcatc cactccacca caaaatcagc cagcccctca acatcattaa tatccagtaa
      121 cgcgacatca agattaagcg gcacatcact ggctacagca ataacatgcc tgtctatcac
      181 taattcttcc ggtcgatgtc cggctccatc gcgaaacagc acaatctttg cgatctcttc
      241 atgcttaaac ccttcgacca gaatcaaatc cagctttgag gtatccattc gacttgcgag
      301 aaaatgtaga tccagctctt cttcgtctgg tgtttccgtc atcaaggccc atcgctgttg
      361 gctggcaacg atggtttgcg ccgcgccagc cttgcgcagc tcataactat ctttgcccgg
      421 tttatcaaca tccatatcat ggtgcgtatg cttaatcagc cccggacgga tccctctggc
      481 gcataatgcc gggatcaatt ttttcagcag cgttgttttt ccggtgccac tccatgcagc
      541 aaaggcgagt aacggtatca tcgtttttcc tgccatcggg caagctcctc tggcgtattc
      601 acgtttataa atgcctcttt acgatcgctg aaatcaaccg catgaccgcc agccagacgc
      661 ataaatgcca taacccggcg ttctcctgct tgcagatatt ccagtaataa aggctcaatt
      721 gcgcggttta ccagagcaat agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca
      781 ggcgcatctt tgcgctgatg agtaagccgg gcggctaaat catggggaat gtaaggcgta
      841 tcgcacgggc aaaacaaaaa ccactcaccc gcttcctgct gcattactga aagcattcct
      901 gccagaggac cagggtaatc cgccagtgaa tcttcaatca ctttcagacc gcttacctga
      961 tagatttcct gatgacgatt agcattaacc acgacgtgag aaagctgcgt cataagcgcg
     1021 tcagcgacat actgccataa tggtttgccg tttaattcaa gcaatccttt atctacgccg
     1081 cccattcgtc tggctttacc gcccgccagc acaacgcctg ttatcatcgt catcagattc
     1141 accgatatcg cctcttttat tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa
     1201 ggagcattac tatgaaatgt aaacgtctga atgaagttat tgaactcctc cagccagcct
     1261 ggcaaaaaga gccagacctt aacctgctgc aatttttgca gaaactggcg aaagagtcag
     1321 gttttgacgg cgaactggcg gatttgacgg atgacattct gatctatcac ctgaaaatgc
     1381 gtgattccgc gaaagatgcg gtgatcccgg gtttgcagaa agattatgaa gaagatttca
     1441 aaacggcact attacgcgca cgtggcgtaa ttaaagagta aaagcttgta agcggcgcaa
     1501 ccaaaatcat cgtgaaatga tatccttcat cattcgtaat gttttccgga tgatgggatg
     1561 aacaacagcg cttttacttt ccagacacta cacccggata ccatcatgga cgctctgttt
     1621 gagcatggga ttcgggtgga ttccggtctt accccgctta acagctatga aaaccgtgtc
     1681 tatcaatttc aggacgaaga tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt
     1741 tggacagccg atcaaatcct cgaagaacat caatttgcgt tgcagctggt taacgatgaa
     1801 gttccggtcg cagcacctgt ggcctttaat ggtcagactt tattgaacca tcagggattt
     1861 tatttcgctg tttttccaag cgtcggtggt cgccagttcg aagctgataa tatcgatcag
     1921 atggaagcgg ttgggcgtta tttagggcgt atgcaccaga cggggcgcaa acagcttttt
     1981 atccatcgcc cgaccatcgg tttgaatgaa tatctcattg agccacgcaa gctgtttgag
     2041 gacgccacac tgatacctcc cgggttgaaa gcggcattcc tgaaagcgac agatgagctg
     2101 attgccgccg ttacagcaca ctggcgggaa gatttcaccg ttctgcggct acatggagac
     2161 tgccacgccg ggaatattct ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca
     2221 cgtaatggtc cagccgttca ggatttgtgg atgttgctca atggcaataa agccgagcag
     2281 cggatgcaac tggaaactat tattgaggct tatgaagaat ttagcgagtt cgacaccgct
     2341 gaaatcggac tgattgaacc tttacgcgcc atgcgtttgg tttattatct tgcctggcta
     2401 atgcggcgtt gggctgatcc cgcgttcccg aaaaatttcc cgtggttaac cggggaagat
     2461 tactggctac gacagacggc gacttttata gaacaggcaa aagttctaca agaaccccct
     2521 ttgcaattaa cacctatgta ttaatcggag agagtagatc atgaaaaaga tttggctggc
     2581 gctggctggt ttagttttag cgtttagcgc atcggcggcg cagtatgaag atggtaaaca
     2641 gtacactacc ctggaaaaac cagttgctgg cgcgccgcaa gtgctggagt ttttctcttt
     2701 cttctgcccg cactgctatc agtttgaaga agttctgcat atttctgata acgtgaagaa
     2761 aaaactgccg gaaggcgtga agatgactaa ataccacgtc aacttcatgg ggggtgacct
     2821 gggcaaagag ctgactcagg catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt
     2881 cacagttccg ctgtttgaag gcgtacaaaa aacccagacc attcgttcag catctgatat
     2941 ccgcgatgta tttatcaacg caggtattaa aggtgaagag tacgacgcgg cgtggaacag
     3001 cttcgtggtg aaatctctgg tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg
     3061 tggtgttccg gcgatgtttg ttaacggtaa atatcagctg aatccgcagg gtatggatac
     3121 cagcaatatg gatgtttttg ttcagcagta tgctgatact gtgaaatatc tgtccgagaa
     3181 aaaataattc aatgtaaatt acataaagcc cg
//
LOCUS       DADTFG010000258         3164 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.260, whole genome shotgun sequence.
ACCESSION   DADTFG010000258 DADTFG010000000
VERSION     DADTFG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.260"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..1170)
                     /locus_tag="J1138_004575"
     CDS             complement(<1..1170)
                     /locus_tag="J1138_004575"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012337.1,HMM:NF014819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HBA8456251.1"
                     /translation="MRNKVVVTGMGIISSVGHTPEECHLTLLKGKGMTRLDSRLQGLK
                     VSWSCPVQNFAPAQHMSKSLIWRTDPFIQYALYAACNAVEDAGLKKGYGGLKTGIVLG
                     NSLAGISSLETLNKRAFQEGTATIGSLYLLSTMGSMATGNIAIELGIHGPTYLTGTAC
                     SSGADAIGTASRLIESGECDIVIAGGSEAPITPLVVSSFTKLGALSKNTDLPHASRPF
                     DKERDGFVISEGAAMMVLENEKTAMERGARIYATLSGYGSANDAHHITSPAPDGSGLS
                     KAIYRALEDASLSSCDIQCINAHGTSTPLNDYLEGNTLSAIFPDHPWVTSTKGVTGHS
                     LAATGAMEAIFSVLNIIHDEITPVAGLKNIDEAIKINIVTEVIKGAGIKNVLSTSL"
     gene            complement(1188..1412)
                     /locus_tag="J1138_004576"
     CDS             complement(1188..1412)
                     /locus_tag="J1138_004576"
                     /inference="COORDINATES: protein motif:HMM:NF012759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA8456252.1"
                     /translation="MDKLKELLTEVFGVPENNIQDNKSLELIGLDSISIVEFQIEIER
                     AFKIDEGKLALVNQDTLNTIKERVKVLQNV"
     gene            complement(1405..2439)
                     /locus_tag="J1138_004577"
     CDS             complement(1405..2439)
                     /locus_tag="J1138_004577"
                     /inference="COORDINATES: protein motif:HMM:NF015700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456253.1"
                     /translation="MKYYSFAKFQTPHVLLPYKEDILSKLTVLFISRRCLININSGGT
                     VKRIKDAKLYTHKFHESEIFIKNTSRISDNHFQANFRLIKNHGFYSDFLACQSKINPF
                     FLLECARQAETVLSHTEFAIAVDSKFLLDRWSITYYPTGFDDTGTLKADVYTKEPTTK
                     KTNKNTFNIVFKLKETVIGVVKIEVRYITNSCYKRIRRIANGNTFPKISTPLLPATVC
                     FTSQENVVLADLSDEGPYVKATIYISNDNKSLNDHEQDHITGMNLTEAAKQICYSYIS
                     LYMKEKNNCYIPIKLEANFYSYVEKHIPAVLVIKKVTLQDNLYLFDVDIIQNEKTLAT
                     VNLKLGGEYG"
     gene            2680..2883
                     /locus_tag="J1138_004578"
     CDS             2680..2883
                     /locus_tag="J1138_004578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456254.1"
                     /translation="MPEGMTLFAISGSTRDVRIQSRLQGEVIESLSKKHDVLDDLEDE
                     KRPVDEKPYQRLLIRPGSLQDYS"
     gene            complement(2885..>3164)
                     /locus_tag="J1138_004579"
     CDS             complement(2885..>3164)
                     /locus_tag="J1138_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBA8456255.1"
                     /translation="GYNTKQDICGHGFRAMACSALMESGLWSQDAVERQMSHQERNTV
                     RLAYIHKAEHMEARMDMMQWWSDYLDMCSEIWVPPYIWSQQNINLAVT"
BASE COUNT          862 a          660 c          628 g         1014 t
ORIGIN      
        1 cagggatgtg ctgagtacat ttttaatgcc tgcaccttta ataacttccg ttacaatatt
       61 tatttttatt gcttcatcaa tatttttcaa gccagcaaca ggagtgattt catcatgtat
      121 aatattcaag actgaaaaaa tagcttccat tgcacctgtt gcagcaagac tatgtccggt
      181 aacacctttt gttgatgtca cccaaggatg atcagggaat attgcagata aagtatttcc
      241 ctcaaggtaa tcattcagtg gtgttgatgt gccgtgagca ttaatacact gaatatcaca
      301 cgatgataac gaagcatctt ccagtgctct ataaattgcc ttagatagcc cagaaccatc
      361 tggtgctggc gaagtaatat gatgcgcgtc gttggctgat ccgtatccgg ataacgttgc
      421 ataaattctg gcaccgcgct ccatcgctgt tttttcgttt tctaatacca tcatcgccgc
      481 accttcggag atgacaaaac catccctttc tttgtcaaac ggacgtgacg catgcggcag
      541 atcagtattt tttgaaagag caccgagttt cgtgaaagac gaaacaacca gtggtgtaat
      601 cggcgcttca ctgccaccgg caataacaat atcgcattct ccgctctcga taagacgact
      661 tgctgttcca attgcatctg ctccggaaga gcatgctgta cccgtaagat aagttggtcc
      721 atgtatacca agttcaatgg ctatatttcc tgttgccatg ctgcccattg tggacaagag
      781 atacagtgac ccaattgttg ctgttccttc ttggaaggct cttttattca gggtttccaa
      841 agagcttatg cctgcaagag aattgcccag tacgatgcct gtcttcagcc ctccataccc
      901 cttttttaat cctgcatctt caacagcgtt acaggctgca tacagggcat attgaataaa
      961 agggtctgtt cgccagatca aagatttact catatgctgt gcaggggcga aattctgaac
     1021 aggacaagac catgagactt tcagaccttg cagacgcgag tcaagtctag tcattccttt
     1081 tcccttcaat agagtcaaat ggcactcttc cggtgtgtgc cctacagagg agataatccc
     1141 catgcctgta accacaacct tatttctcat gttaatggac ctttgattta tacattttgc
     1201 agcactttga ctctttcttt aattgtgttg agggtatcct gattgaccag agcaagtttc
     1261 ccttcatcaa tcttaaaagc tctttctatt tcaatttgga attccacaat actgatggaa
     1321 tcaaggccta taagctcaag ggatttgttg tcttggatat tgttttctgg aacgccaaac
     1381 acctctgtta ataactcttt taatttatcc atattctcct cctagtttta aattaacggt
     1441 tgccagtgtt ttctcattct ggattatatc aacatcaaat aagtaaaggt tgtcctgtaa
     1501 cgttaccttt tttataacta aaactgcagg tatatgcttt tctacataac tgtaaaagtt
     1561 tgcttccagc tttattggaa tatagcagtt gtttttttct ttcatgtata aggagatgta
     1621 gctatagcat atttgtttag cagcttcagt tagattcatt ccagttatat gatcttgctc
     1681 atggtcatta agtgatttat tatcgttact aatataaata gttgctttaa catatggacc
     1741 ttcatcactt agatctgcca gcaccacatt ttcctgcgat gtgaaacaga ccgtagcagg
     1801 taatagtggt gttgaaattt tagggaaggt gttcccatta gcgattcttc gaatacgttt
     1861 atagcaagaa tttgttatgt accttacctc aattttaacc acgccgataa ctgtttcttt
     1921 tagtttaaaa acaatgttaa acgtattttt gtttgttttc tttgttgttg gttcttttgt
     1981 gtagacatca gcttttaatg ttcccgtatc atcaaaccct gttgggtaat atgttatgga
     2041 ccacctgtca agcaaaaact tactgtctac cgctatagca aactccgtat gcgataatac
     2101 tgtttcagcc tgcctcgcgc attccagtaa aaaaaatgga ttaatcttgg actgacaggc
     2161 gagaaaatca gaataaaacc catgattttt aattagcctg aagttagctt gaaaatgatt
     2221 gtcagaaatc cgtgaggtat tttttatgaa tatctcactt tcatgaaatt tgtgtgtata
     2281 taatttagca tcttttattc ttttcacggt accccctgag ttaatattta ttaaacacct
     2341 cctgctgata aataaaactg tcagttttga caggatgtct tctttatatg gcaggaggac
     2401 atgtggcgtt tgaaactttg caaaggaata gtatttcata atgctcagga ttataataaa
     2461 atctggagtg acgggataga aacagagaaa tgtactgcat taatccagtg tgttgtggat
     2521 ttgccaccac tattttttct atactggata tgaataacct gcatcagccc tgactagggc
     2581 aacgacaata tcgaggaagg caacaggcgt gaatggttct tgcatgtaac tacctctggc
     2641 tatcaaattg attgatattg tcagtagtaa gagatagtga tgcctgaagg gatgaccctg
     2701 tttgctattt ctggtagcac aagggacgtg cgtatccaaa gtcggttaca gggtgaagtc
     2761 atcgagagtt tgtcgaagaa acatgatgtc ctggacgact tagaagatga aaaacggcct
     2821 gtggatgaaa agccgtatca gcgtttgctg atacggcctg gttctttaca agattattca
     2881 taagtcaggt tactgcgaga ttaatgtttt gctgactcca gatgtacggg ggcacccaga
     2941 tttcactgca catatccaga taatccgacc accactgcat catgtccatg cgggcttcca
     3001 tatgttcggc cttatgaatg taagccagac gcacagtgtt gcgctcttgg tgactcatct
     3061 gacgctccac tgcatcttgc gaccataaac cggattccat cagcgcgcta cacgccattg
     3121 cccggaaacc atgaccgcag atatcttgtt tcgtgttgta tccc
//
LOCUS       DADTFG010000259         3158 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.261, whole genome shotgun sequence.
ACCESSION   DADTFG010000259 DADTFG010000000
VERSION     DADTFG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.261"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..770)
                     /locus_tag="J1138_004580"
     CDS             complement(<1..770)
                     /locus_tag="J1138_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HBA8456256.1"
                     /translation="MGNYHIGDGECRFRVVCSSPDVCEVGGYKVPFDSYQTLDSERQY
                     SSTVWARGCRALNVGSVIAGTQSNAGKGVISGTSQGTGDCVILTGSPTVTIEGKPVAY
                     HGSVVGINNHNCLGKLYTKIKSPMISVIDRTFNYERTAEVIHDLLLLKDLLSVGNIFD
                     GDISPEVKNDLFQIKDPDQSWGEFFSIKNIRESLRNGIEGDKSQIREWFGENTLTQMG
                     NGAITTLHGVADLALVTFDALLDTATATVACPIGEDGL"
     gene            complement(763..1809)
                     /locus_tag="J1138_004581"
     CDS             complement(763..1809)
                     /locus_tag="J1138_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HBA8456257.1"
                     /translation="MIEMKNNTPFPLLSFEKYGRYGLLFDVIAIKMSLRIKNGFYADL
                     AEFQKELSMSDEYYGESETSSLKSETDLVLCKRNTDIHVTGSAHAPSGDKSQWKACVR
                     VNSFSKELSLSGVRYLQYERNRWQMSLPDKIINVPLRYELAYGGIWQPDGMEKLVFSA
                     NPVGCGYYPDISQLNTSCQYKLPQITSSALSENATIFNGESDFFQGVGPVSRWWKSRL
                     QYAGTYNEVWREKRYPYLPDDFDERFYNSAHPDMIYTGFLSGDENISLEGFFKFEQVV
                     KTKLPGIRPVLILKTKHNTSHMFLPVADTMVIDLSRQEIYLTWRLTIPDFFGMKEGVL
                     SCIIPECIGKKYYG"
     gene            complement(1916..>3158)
                     /gene="tssI"
                     /locus_tag="J1138_004582"
     CDS             complement(1916..>3158)
                     /gene="tssI"
                     /locus_tag="J1138_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBA8456258.1"
                     /translation="LNGQLEQYEIFDYPGRYKDEQHGKDFTLYRMESLRSDAEKATGQ
                     SNSPKLWPGTRFTLTGHPQKMLNREWQVVQSILSGSQPQALHGSQGRGTTLGNQLEVI
                     PADRTWRPRLQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVKFHWDRYHGMTEESSC
                     WVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPVVMGRTYHEDNRSPGDLPGTK
                     TQMTIRSKTYKGSGFNELRFEDATGGEQVYIHAQKNMDTEVLNNRTTDVKADHTETIG
                     NDQKITVGLGQTVNVGSKKEGGHDQKVTVANDQHLTIKNDRHKVVNNNQTSKVTGTDT
                     EEVVKKQSIKIGDNYELKVEHGTNIISGDSIELICGQGESGTCSIKLEKTGKIIIRGT
                     EFLFEATGPVDIKGKDIHLNG"
BASE COUNT          821 a          841 c          554 g          942 t
ORIGIN      
        1 cataacccat cttcgcctat cgggcaagca accgttgccg ttgccgtatc taacagagca
       61 tcgaatgtaa ctaaggctaa atccgccaca ccatgtaaag tggtgattgc tccattcccc
      121 atttgagtaa gtgtgttctc tccaaaccat tctctgatct gagatttatc tccttcaata
      181 ccattcctga gagattctct gatattcttt attgaaaaaa actcccccca tgattggtca
      241 ggatctttta tttgaaataa atcattttta acctcaggtg aaatatctcc gtcaaaaata
      301 ttaccaacag agaggagatc cttcagaagc agtaaatcgt gtattacttc agcagtcctt
      361 tcataattaa aagttctatc aatgacagaa atcattggtg attttatttt ggtatacaat
      421 ttaccaaggc agttgtggtt attaatccca acaactgaac catgataagc aacaggtttc
      481 ccttctattg ttacagtcgg acttcccgtt aaaataacac agtcacccgt tccttgagat
      541 gtaccggata tgacaccttt accggcatta ctttgagttc cagcaatcac tgaaccaaca
      601 tttaaagctc tgcatccacg cgcccatacc gttgaactgt actgtctttc actatctaaa
      661 gtctggtaac tgtcaaaagg aaccttgtaa cctcctactt cacatacatc tggtgaagaa
      721 cacacgactc taaatctaca ttcaccatcc ccgatatgat agttacccat aatatttttt
      781 ccctatacat tcaggaataa tacaactcag cactccttct ttcattccaa aaaaatcagg
      841 tattgttaat ctccaggtaa ggtatatttc ctgtcgactt aaatcgataa ccattgtatc
      901 cgcaacaggt aagaacatat gtgatgtatt atgcttggtt tttaatataa gtacaggtcg
      961 tatacctggc aattttgtct taacaacctg ttcaaattta aaaaaacctt caagagagat
     1021 gttttcatcg ccagagagaa accccgtgta tatcatatcc ggatgagcac tattgtagaa
     1081 ccgctcatca aaatcatccg gcaaataggg atagcgcttt tcacgccata cttcattgta
     1141 ggttcctgca tactggaggc gacttttcca ccatcgagat acagggccaa caccttgaaa
     1201 aaagtcagat tccccattaa aaatggtagc gttttctgac aaagcagagc tggttatttg
     1261 tgggagttta tattgacaac ttgtattaag ttgagaaata tcaggataat atccacaacc
     1321 tacagggttt gctgaaaaaa ccaatttctc cataccatct ggttgccaaa taccaccgta
     1381 agcaagctca tagcgtagag gaacattaat aattttatct ggtaagctca tctgccaacg
     1441 atttcgctca tattgtaagt agcggacacc agataaactc agttctttag aaaaactatt
     1501 aaccctcaca caagccttcc actgagactt atcccctgaa ggggcatgag cactacctgt
     1561 cacgtgaata tctgtatttc ttttacaaag aactaagtct gtttcagatt ttagactact
     1621 tgtttcagac tccccatagt attcatcaga catggacaat tctttttgaa actcagcaag
     1681 atcagcataa aatccatttt taattcgcaa tgacatttta atagcaataa catcaaacaa
     1741 aagaccataa cgaccatatt tttcaaatga caacaaagga aatggcgtgt tatttttcat
     1801 ttctatcatg cttattgatt cctgaataag tcatgctatg attaatttaa acaatcacaa
     1861 atggtgattc acaggcgtat aaagcaaata caatcaccat gttttatatc ctgcactatc
     1921 cgttcagatg aatatctttt cctttaatat ctacaggacc ggtagcctca aatagaaact
     1981 ccgtcccccg tattattatc tttcctgttt tctccagttt tatggaacaa gtcccgctct
     2041 ctccctgccc acaaattagc tcaatactgt ccccagatat gatatttgta ccatgctcaa
     2101 ctttcagttc gtaattatca cctattttaa tactctgttt tttgacgact tcttcggtat
     2161 cagttcccgt caccttgctt gtctggttgt tatttacaac cttgtgccgg tcatttttta
     2221 tcgtgagatg ctgatcgttc gctaccgtca ctttctggtc atgaccacct tcctttttgc
     2281 tccccacatt caccgtctgg cccaacccca ccgttatctt ctggtcgtta ccaatggtct
     2341 ccgtgtggtc agccttcaca tccgtcgtcc gattattcag cacctccgta tccatgttct
     2401 tctgcgcgtg gatatagacc tgttcaccgc ccgtggcgtc ttcaaaccgc agttcattaa
     2461 agccgcttcc tttataggtc ttcgaccgta tcgtcatctg cgtcttcgtc cccggcaggt
     2521 cacccgggga gcggttatct tcatggtacg tccgccccat gaccaccggc tggtccgggt
     2581 ctccgttcag gaagtccact atcacttcct ggcccacgcg cggtatcgcc aggttaccga
     2641 accccggccc cgcccacgcc tgtgacaccc tcacccagca cgaactctcc tccgtcatcc
     2701 cgtgataacg gtcccagtgg aacttcaccc gtacccggcc atgttcatcg cagaagattt
     2761 cctctcccgc aggaccggtg acaatggcgc tctgtggccc gtccaccttc ggtttgcttt
     2821 gcagccgtgg acgccacgtc cggtctgccg gtatcacctc taactgattt cccagcgtgg
     2881 ttcctctccc ctggctgccg tgcagcgcct gcggctggct tcctgacagt atgctctgta
     2941 ccacctgcca ttcacggttc agcatcttct gcggatgccc cgtcagcgta aaccgcgtcc
     3001 ccggccacag cttcggcgaa ttactctgcc ctgtggcctt ttccgcatca ctgcgcaggc
     3061 tctccatccg gtacagcgtg aaatccttgc cgtgctgttc atccttgtac cttcccggat
     3121 aatcaaatat ttcatactgt tcaagctgtc cgttcagg
//
LOCUS       DADTFG010000260         3153 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.262, whole genome shotgun sequence.
ACCESSION   DADTFG010000260 DADTFG010000000
VERSION     DADTFG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.262"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(118..1140)
                     /locus_tag="J1138_004583"
     CDS             complement(118..1140)
                     /locus_tag="J1138_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HBA8456259.1"
                     /translation="MDKRVIFAVAGSGKTTLLIQRLREGRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPDGIRVMTWFEFLHGFCFRPFLQEQLASRGLSFDQPPPRIPRTNARHYQD
                     LAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELLVDEVQDFAGHDFNFLLELCRAEV
                     SVLCCGDFFQHTFDTSHDGNVNATLHDDITRYEDRFRMAGIIVDCETLSRTWRCSVTV
                     CEFITGQLNIRIAAHGTHTTQIETVTDTERTAALHADDTVIKLFYREHHRYGCHSLNW
                     GVSKGLDHFQDVCIVMGASHWKLLIRQELMALPPSSRNRLYIACSRARGNIYFVPESH
                     LRRFRN"
     gene            complement(1125..2687)
                     /locus_tag="J1138_004584"
     CDS             complement(1125..2687)
                     /locus_tag="J1138_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA8456260.1"
                     /translation="MPLITRLVLQNFKKFPELDLRFTHDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRQFQEGERRVDQLPVLTADVFLSNGGDPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHNVLQQDPDNFPYEYYSVRFSTFSGGHFAGFR
                     RYLRHLLLDSARIDNEHAAQEYTRTVYNVNVPVADRYRLENNYRQQKIHFCTLHLSAI
                     NDTLETYRFGVRSGDKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGEL
                     HALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGSARP
                     VLMNELSAETSAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPEDD
                     GVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVVCSRSRVFADR
                     DNTRSTFEICLYQDNAGLCDALFRGTRRTLTVQEYMLANKAEAAFRLLQLHAEELTVP
                     DYIQEALAWIRE"
     gene            complement(2760..2882)
                     /locus_tag="J1138_004585"
     CDS             complement(2760..2882)
                     /locus_tag="J1138_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HBA8456261.1"
                     /translation="MGSNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR"
BASE COUNT          743 a          845 c          814 g          751 t
ORIGIN      
        1 gttttcacga tcagatccgt tttctgctaa agtcggatcc gtattttccg acctgaagtt
       61 tcgtcgttaa ccgtacagct tttttgcctg actgagttct acagagaagc agtagactca
      121 gtttctgaac cttcgcagat ggctttccgg gacaaaatag atgttgcctc gcgcccgtga
      181 gcaggctatg tataaccggt tacgggaaga cggcggaagt gccatcagtt cctgccggat
      241 gaggagcttc cagtgactgg ctcccatcac gatgcagacg tcctgaaaat ggtcgagtcc
      301 tttactgacc ccccagttca gtgagtggca accgtaacgg tgatgttcac gatagaagag
      361 cttgatcacc gtgtcgtccg cgtgcagggc tgctgtacgc tctgtatctg tgactgtttc
      421 aatctgggtt gtatgtgttc catgggctgc aatacggatg ttcagttgtc cggtaatgaa
      481 ttcacacact gtaactgaac agcgccatgt ccggctgagc gtctcacaat ccacgatgat
      541 gcccgccatc ctgaagcggt cctcgtaccg ggttatgtcg tcatgcagcg ttgcattgac
      601 gttaccatcg tggcttgtgt caaaggtatg ctgaaaaaaa tcgccgcaac acagcacaga
      661 gacttccgcg cggcaaagct ccagcagaaa attaaagtca tgccccgcaa agtcctggac
      721 ttcatcgacg agcagttcat cgtagtaacg ggcaagccgg gtacggatat ccggcagcaa
      781 tccccgggct gtcagcaagt gtgcgagcct tcggtgatac agtcgtccgg cgagatcctg
      841 ataatgccgt gcattcgtgc gcggtattct gggaggcggc tggtcgaagc tcaggccacg
      901 cgatgccaac tgctcctgca ggaatggacg aaaacagaaa ccgtgcagaa actcaaacca
      961 ggtcatcaca cggatcccgt ccggtatgta gccaaagcgt ctgataatct gcgcccgcag
     1021 gtgcgcttca ttattcacag tgaaggtcag gatcagcgtc cgccggccct cccttaaccg
     1081 ctgtatcagc agcgtggttt tgcccgatcc ggcaaccgca aatatcactc tcttatccat
     1141 gccagtgcct cctgaatata atcaggtact gtcagctctt cggcgtgcag ctgtaacagc
     1201 cggaatgctg cttctgcctt attggccagc atgtattcct gcaccgtgag tgtccggcgg
     1261 gttccgcgga aaagcgcatc acacagacca gcgttatcct gatacaggca gatttcaaat
     1321 gtggatcggg tgttgtcgcg atcagcaaat acccgggagc gggaacaaac cacatcagca
     1381 tagcgttcat cgcagttttg ctgatagttg ccgtcattat cacgaagggc cgcgacgcga
     1441 ttttctagaa gacgggccag ttcaagataa cgacggaaac ttgttccgcc gatagcgatg
     1501 atgtgaaccc cgtcatcctc cggcgccctg ccatacaggc ggtgataaaa ggcctcgatc
     1561 agaataaact cagcatcccc ctccacgagc agcacacgtc tggccagcgc aaattccagc
     1621 acgttattat ccggtgcttt catgaagaag gcggaagtct cagcggaaag ttcattcatc
     1681 agtaccggcc gggcagaccc gaggaggatt gccttacgca gatcgaggcg ggaggagata
     1741 tggctgctat gtgtggcaat aaacacctgc gtctgccgtt cagtggcaag ctgattaacc
     1801 agcctcttca tactgacatg gctgagatga ttctccggct cctcaagcaa cagcgcgtgg
     1861 agctctccct gctgctgatg ccgctgcagc gcaaactccg ttttgatgaa acactgcctt
     1921 ccttttccac ggtgacgaat ggaaataccg tcctcggtaa tatccagatt cgcctcaaga
     1981 cctgatttat cgccggaacg cacgccaaac cgatacgttt ccagcgtgtc gtttatggca
     2041 gacagatgca gggtgcagaa gtggattttc tgttgccggt aattgttttc aagccggtag
     2101 cgatcggcca caggcacatt gacgttgtaa actgtgcgcg tatattcctg tgcggcgtgc
     2161 tcgttatcga tgcgtgcact gtctaacagc agatggcgca gatagcgtct gaagccagcg
     2221 aaatgcccac cggagaaggt gctgaaccgc actgaatagt attcataggg aaaattatcg
     2281 ggatcctgct gcaggacatt gtgaatatcc tgaccgtatt cctccatcat tggtgcaatg
     2341 cgcattctga gaccatccgc atcgataccc gccagatttt gtctgccgtt caggtcagga
     2401 tctcccccat tactcaggaa aacgtcagct gtcagcacgg gtaactgatc gacgcgacgc
     2461 tcaccttcct gaaactgcct gactgctgac tgggacagga gcgattccac acccaatgcc
     2521 tcgacccggt gccggctgtc actcagcaca aggtcaaggg ccagcagaat agtgcttttt
     2581 ccggactcat tgtccccgac cagaatgtta cggtcatgag taaagcgcag gtccagttcc
     2641 ggaaactttt taaagttttg cagcacaagc cgcgtgatca gaggcatctt ttattccttt
     2701 ataaaccgct gactgaaaga gtgtcggcag cgccagactg cctgttttgt tttacagggt
     2761 tatcttaccc agtcttccag cacaagaccc ggtacccgtt caaattcccg cgtattattc
     2821 gtcaccagca cggcaccggc agcgatggcg tgcccggcaa tagccgtgtc gtttgacccg
     2881 atcggcgttc gcccatgcgc agcgccacct taatttccgt ggtcgcatcc accggtatag
     2941 atgtggcata tgcagatgat gtgtatatgc gtatgccaag agtatggata tgccgaacat
     3001 gaatgcggtt ctctcttttt cagctgagat ccgaaaaagg ttaaacagga ttttagaatg
     3061 acaaagcctg tatgaagggc atcgggtgac attttccaga gaaataattc ccccaacggc
     3121 ttattattca tggaatttct tatggataaa gca
//
LOCUS       DADTFG010000261         3130 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.263, whole genome shotgun sequence.
ACCESSION   DADTFG010000261 DADTFG010000000
VERSION     DADTFG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.263"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            53..946
                     /locus_tag="J1138_004586"
     CDS             53..946
                     /locus_tag="J1138_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8456262.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGMPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(977..1966)
                     /locus_tag="J1138_004587"
     CDS             complement(977..1966)
                     /locus_tag="J1138_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA8456263.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGNDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(1993..2844)
                     /locus_tag="J1138_004588"
     CDS             complement(1993..2844)
                     /locus_tag="J1138_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA8456264.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT          756 a          749 c          798 g          827 t
ORIGIN      
        1 gccccccttc atgagtgtct gtgaagagga tgcttctaca aggaaatcgc caatgctcaa
       61 agaaaacttc aacgaactgc aaatctttct tgtggtggca agagagcgaa gttttaccaa
      121 agcagcgggc aaactcggcg tctctcagtc tgcacttagc catgcaatga aggctctgga
      181 ggaaaggctc aatatccgcc tcctgacacg gactacccgc agtgtggctc ccacagaagc
      241 gggggagaga atcattgcct gccttgaacc gcgtattgac gagcttgaac aggaactgga
      301 atcacttatt caattgaacg gcatgccctc cgggaatatt cggttatctg ccggggaaca
      361 tgccgcgcga agtctggtct ggccgaagct aaaacctttt ctcagagagt atcccgaaat
      421 caatgtcgaa ctggtggtgg ataatggttt tgtcgatatt gttgaaggcc gttttgatgc
      481 cgggatacga ctgggtgaaa atgttgataa agatatggtt gcagtcagga ttggaccaga
      541 tatgcggatg gctgtggtgg gagctccggc gtattttgct gcaaaccctg cgcctgaaac
      601 gcctcatgag ctacaaaatc atcgctgcat caacatgcgt ttgccaactg ccggtgggct
      661 ttaccactgg gagttcgagc gagaagggaa accgcttcgc gttaaggttg atgggcaact
      721 gacgtgtagt ccattgccag aaagaattga tgccgcgtta tcagggtttg ggatcgcttg
      781 tgtccctgaa gacatggttc aggagtatat agagtcaggc aagcttattc aggtgttaca
      841 ggagtggtgt ccaacttttc ctgggtatta tctttactac cccagccgca agcagcatcc
      901 gccagctttt gcgttgttga tcgatgcgct tcgctacacc gaataacggg tcctttatcc
      961 tggacccgtt tgggtattag cggcctacgc gtgcctgatg ttcaggcgag taacgttcac
     1021 cgacgatttt aattgtttcc agcgcctcgc ttatctgctg cgtatctttc tgcgaaagaa
     1081 caatgtcagc agcggccagg ttttcttcca gccggtgcag tttagtggtg ccagggatgg
     1141 gaacaatcca cggcttttgt gctaatagcc atgccagtgc gatttgtgca gacgttaccc
     1201 ctttctccgc agccagttca cctaacaagg tgaccagttt ttcattggct tcaatggctt
     1261 gtgctgcgaa acgcggtacg gtgctgcggt aatcatcttt gccgaaagtg gttcccggct
     1321 taatcgcccc tgtcaggaaa cctttaccta gtgggctgaa ggggacaaaa ccaattccca
     1381 gttcctcaag caacggcaga atttcctgtt caggctcacg ccaccacatg gaatattcgc
     1441 tttgcagggc ggtgacaggt tgtaccgcat gtgcacgacg aatggtttgt gctcctgctt
     1501 cagacaggcc gaaatgtttg accttacctt ctgcgatgag gtctttcacc gttcctgcaa
     1561 catcttcaat aggaacatct ggatcaacac ggtgttgata cagcaggtca ataacatctg
     1621 ttttcagacg acgtaatgat ccttcaacgg cttcacggat atgttccgga cggctgttta
     1681 aaatctgctg cttattatca ttgccgaagg taaaaccgaa tttagtggca attaccacgc
     1741 ggtcacgaaa cggttttaag gcttcgccga ccacttcttc attcagatac ggaccataca
     1801 cttcggcggt atcgaagaag gtgacgccac gttcaaccgc agcacgaata agttcgatag
     1861 cctgacgggt atcggtcgcc gggccgtagc cgtggcttaa gcccatgcaa ccgagcccaa
     1921 gggcggagac ttcgagtccg gatttaccca gataacgttt ttgcattgaa tgaatacctc
     1981 ttttatcgcg gcttaaacat caagtttgcg gccagtcagc cattccacca ttgcgggatc
     2041 gcggtgagag aagaacgcgc tggttgcggt atccagggcg gtaatttgca gcatatcttc
     2101 agggctgagt tcaaaatcga gaatgttgat gttctcttcc atgcgttctt ttcgcactga
     2161 ttttgccagt gaaacaatgc ctcgttggta gatccagcgc agaacaacct ggcccacgct
     2221 tttgccatac ttctcgccaa ttgccgtgag aacaggatgc tggaacagac catttttccc
     2281 ttccgcaaac ggtgcccagg cttctggctg aatgccacga ctttgcatcc agggaacggc
     2341 ctgtagctgc tgattgaaag ggttaacttc aacctggttt accgccggaa cgacgttatt
     2401 aaaagcgata aggtctgcca gtcggtcagg atggaagtta ctgacgccaa tagcgcgaat
     2461 tttaccggcc tgttgcagct cttccatggc acgccaggct ccgtggacat cgccatatgg
     2521 ctggtgaatc aagtacaggt caacgtaatc aagttgcagt cgattcagag agcgttcgaa
     2581 ctgggctttg gccccttcgt aactggtatc ctgcaaccag agtttcgtgg tgacaaagag
     2641 ttcgttacgg gcgataccgc tttgtttcag tgcgttcccg acctggattt cattttgata
     2701 agaggcggcg gtgtcaatca gtcgataacc gctgttaata gcgtcaatga ccgctctttc
     2761 gcattcggcg gcatcggtca tctgaaatac accaaagccc aacaggggca tttcaatacc
     2821 gttgttcagt tttacttttt gcatggtgtt atccttccgt tatcgttcat gagaagcata
     2881 acgtaaaggg aagagctcga ttagacggca ggatttgtca ggggttatga acaaaattca
     2941 taaatctgtt tgagggttga gattagtcgt gcaagttcga tatccccgga atttacagtc
     3001 cgatgaataa aaatgaatgt ccataaaaaa tatattggtt tatcccagcg tatatactta
     3061 cttcgacgta ctcccaatag cagttaatta aaggcgggtc attattattc actctgaata
     3121 atgaattatt
//
LOCUS       DADTFG010000262         3052 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.264, whole genome shotgun sequence.
ACCESSION   DADTFG010000262 DADTFG010000000
VERSION     DADTFG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.264"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            22..1128
                     /locus_tag="J1138_004589"
     CDS             22..1128
                     /locus_tag="J1138_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBA8456265.1"
                     /translation="MLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVG
                     LVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIP
                     ELVPNKDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGV
                     ELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIG
                     YRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELI
                     LNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWVTLAESGRSHEIQ
                     TALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1601..1702
                     /locus_tag="J1138_004590"
     CDS             1601..1702
                     /locus_tag="J1138_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456266.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2067..2330
                     /locus_tag="J1138_004591"
     CDS             2067..2330
                     /locus_tag="J1138_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBA8456267.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            2330..2470
                     /gene="rpmJ"
                     /locus_tag="J1138_004592"
     CDS             2330..2470
                     /gene="rpmJ"
                     /locus_tag="J1138_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBA8456268.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(2540..2730)
                     /locus_tag="J1138_004593"
                     /pseudo
     CDS             complement(2540..2730)
                     /locus_tag="J1138_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          892 a          665 c          644 g          851 t
ORIGIN      
        1 ccatcattgt tttctccttt tatgctgaca gaaaaaataa agctacgtaa tcgtatcgtt
       61 atggctccta tgacaacctg gtcagctaat cctgatggaa ccatctctga gcaagaactc
      121 gaattttata aacgtcgctc acaaaatgtt ggtttagtaa taacaggatg tacctatgtc
      181 acgccaagcg gcattgggtt tactcatgaa tttgccgcgt atgacgaccg tttcattaac
      241 agccttgaaa agttagcagc tgcagccaaa agtggcggtg ctccggctat tttacagatt
      301 ttccacgccg gtaataaagc catcccggag cttgtgccaa ataaagatgt tattagtgcc
      361 agcgcatcaa gcgtaaaatc tggtgacttt atgaagagag tggtacaaag cagggaaatg
      421 actgaaaacg aaattcagga aaccattaga gcattcggag atgtcacaaa aagggcgatt
      481 aaagcaggtt ttgatggcgt tgaacttcat ggtgcgcacg gattccttct gcagaatttc
      541 ttctcccctc tatttaacca gcgtaatgac cgatggggag gagatcttga aggacgtatg
      601 cgcttcccgc tggccgtatt acaggaagta aaaaatgtag tctatgaata tgcgacaaag
      661 ccctttgcta tcggttacag aatatctcct gaagaatctg taacaggggg actgcggata
      721 gaggatactt ataaactcct tgatcggctc atttcatccg gtatttcgta tatacatacc
      781 tcgctggtca gtattaacga tagctaccct gttgagtcgc caaatggccc ccgaactatt
      841 gaattgattt taaatcatat tgcaggcaga gtgccggtaa tcgccgcagg taaaatacgc
      901 acaccgtctc aggctcaaga agcaatttcc gctggcctgc cacttgttgc gatcggtaaa
      961 gggctggtga taaatccaga atgggtgact ttagctgaat caggtagaag tcatgaaatt
     1021 caaacagccc ttaatccaca acgcgttcct gaattaacca ttccggataa actatgggat
     1081 caaattcagg catcgaaagg aactggctgg tttccgttaa tggattaaat atcgattact
     1141 ctcccgagag taaaactatc aaatcaatga gtaaccgata cagcctcgca aattctttca
     1201 gataattaac ccgtcactgc ggcacgttct ctcgcaaggc gtgcctgcag tgtaatccag
     1261 tatgtatccg gcatagttga cggtttaata gactcaattc ctctccctgg ttacctacaa
     1321 cgatatcttg tataacaccc caggagatac gacgttacga tgagatgcga tatgctcctc
     1381 agcgccaatc aacgccagga ctaggccagt cttgagatca gactcattcc cgtaactgat
     1441 ttcaggcgac atatatcaat gacgttgggg caataaaaga tagccgatgt aatacatcta
     1501 ttcaaatttg tgatacatgt caaaaaattg gttgaccaaa ctcgttattt atataagggc
     1561 acttacgaag tgcactcttt tttaaagcga ggaagtacca atgaaagaga ataaagtaca
     1621 gcaaatcagt cataaactga ttaatatcgt tgtttttgtc gcaattgtag aatacgccta
     1681 tttatttctc catttctatt aataacggca ataaactgtt cacttcagtg atatttaaaa
     1741 tatgcatcct ctcccttttt tgtaagtaat tattatatcc gtgggagagg aatacacatt
     1801 gtcaggtaat caatcatgct gcaataaatc atcggccagt aaagtggaga tagcctccat
     1861 tctcgaaaaa tccatactct cagcgaaacc atcatcaatc actcatccag gcgtttatgg
     1921 gagcgtcgcc aatgggtgct aacaatgcca gacttccccg ttgcggaaat tccacatccc
     1981 acaaatagtc acagtgattg ggtgttgaaa tgatccggat gagcatgtat ctttacggtt
     2041 atgttataac ataacaggta aacatgatga aacccaatat ccatcctgag tatcgtactg
     2101 tggtgttcca cgacaccagc gttgatgagt actttaaaat cggctcgact atcaaaacag
     2161 accgtgagat tgagctggat ggcgtaacgt atccgtacgt aacaattgat gtctcttcga
     2221 aatcgcaccc gttctataca gggaagctga gaacagtggc atcagaagga aatgttgcac
     2281 ggttcaccca acgttttggt cgttttgtta gcacgaaaaa gggggcatga tgaaagtcct
     2341 taactctctg cgtaccgcaa aagaacgcca tccagactgt cagattgtga agcgaaaagg
     2401 acggctgtat gtgatttgta aatctaatcc acgttttaag gccgttcagg gtcgtaagaa
     2461 aaaacgttga tttaaaattc gacgaattaa cgatatttat ctgattaata atcagaccgg
     2521 attaatgttg gtgtatttat tacaccagca ttaattttcc tggggatgta tcttcctgtt
     2581 catttaaggt caactgcctg acgtttctct ccgaatattc cattatatta atgttgactt
     2641 gttgaccagt ttcgccctgt atgctggcat caactctctt ttagactgaa cacgccactc
     2701 agtttcctcc ctttgcgacg cagcctgcat ttacactcaa actgttaaga tgataaatgt
     2761 ggtaaatctg ttggtactga cataaaaacg tttacgccac aggaacggcc agatccatcg
     2821 gtaaccccat cgccgacgtt cgagtgccag ttagagtaac gcgcacagat gactgaatgc
     2881 agtgccctgg caaagaggcc atcgttcctg tgacaactgg cagtcttcgt ttaacttcac
     2941 ttaatttggc tcttgggggg cttaccggac agatgacgta cttacacctg tttaattttt
     3001 catcacttat tgggatgaac acccataacc attttgtgcg gcatgttaat cc
//
LOCUS       DADTFG010000263         3002 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.265, whole genome shotgun sequence.
ACCESSION   DADTFG010000263 DADTFG010000000
VERSION     DADTFG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.265"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            370..677
                     /locus_tag="J1138_004594"
                     /pseudo
     CDS             370..677
                     /locus_tag="J1138_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(842..1981)
                     /locus_tag="J1138_004595"
                     /pseudo
     CDS             complement(842..1981)
                     /locus_tag="J1138_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(1959..2315)
                     /locus_tag="J1138_004596"
     CDS             complement(1959..2315)
                     /locus_tag="J1138_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HBA8456269.1"
                     /translation="MITELSEREYKELTDAAEGQTLDAILCYSVPDIHPAEFSFADRR
                     EIFFWALTRLLKEGRIKLAKHGKFLEGSVDEQVERFRQAFPKTEEEMEDGIWFFDESC
                     PGGAVWVLEDGSLEWT"
     gene            complement(2312..2764)
                     /locus_tag="J1138_004597"
     CDS             complement(2312..2764)
                     /locus_tag="J1138_004597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456270.1"
                     /translation="MVVQVLEYRGGWGPDNEEKARHQEVQQQRFDELSKIYDKSHPAG
                     ELTVDGQTIRQSSVSNRYGTTKVFESQTLTDKQIHNYAQQLAGDTPLKEVKSGIYTSK
                     LSDGSVITLRNISTSEGQTGARWTIDIRNNQKLTELGNKYSRVEIKFK"
     gene            complement(2799..3002)
                     /locus_tag="J1138_004598"
                     /pseudo
     CDS             complement(2799..3002)
                     /locus_tag="J1138_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367177.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin"
BASE COUNT          740 a          836 c          606 g          820 t
ORIGIN      
        1 gagaaaaatg gatatctttt gtctcaatca gataacgcat ataattcgca attgttatgc
       61 attgaaaatg ctaaaaaaca gggatactca gacgaatcgg tcttgccact ttttctacat
      121 atttcctata ttcaggaaaa aggctggaaa tggtatcaat gttatgattg tggatatatt
      181 gtaaaagaaa cctctgtttt tttcccgaca taccaagcat gtatcattga tgctgaacaa
      241 gaatgtattg gtatactagt tcaactgtct gaaaaaactt gaagcgcccc ttgaaacgcc
      301 agacagtgga tgtatcttaa aataagagat aggcttggat atatgtctaa cactagttct
      361 aattttgaga taactggaac tttgctgggg caggagcttc gtaaacgtaa aactcctcag
      421 gaaaaaattg ccattattca gcagacaacg gagcctggca tgagggtgtc ccatgtggcg
      481 catttgcatg gtattcagcc cagtctgttg tttaagtgga aaaaacaata cctggaagga
      541 agtctcgctg ctgttactac cggagaggat gttgttcctg cctcagaact ggctgccgct
      601 ataaaaataa aagagttaca gcccttgctg ggtaaaaaaa catggaagtt gagatcctca
      661 aagaagccgc ggagtacacc actctacagt agttgcgcaa agtcggagtg gacgataatg
      721 tgatcgtcta taagggcaac gctatcatag tcttgttctg gcgcgtaaaa aaacggtaag
      781 cgtaaagcca atgccgtctg taactcctga tccttgcaga ctaagttact tttccaccta
      841 gctaggatcc tatagaaccg ccaatatctc cagcagttcc aagaacattg ctcggtttca
      901 tgccttctcg aatgatatcg ttacgattta cttcagcata ttttatccat gttggatcgt
      961 acttaggatc ccagccattt gtcacccatt ttcctacagc attcgtaata ttatctgtcc
     1021 cccaagtaat acctttaccg ataccatacc cggcagcaga accgatacca tttgaaattg
     1081 caccagacag aggatctttg ttgtcaatcc agttacccag cgcaccacct gccgcattcc
     1141 agcccacagt ccccgccagg ccattaccca tgctgaacac attcacccag cctgcaatgg
     1201 ctgcgtttgt cggatctaca gtaccatcag ccagataact gataccagca ttcgcacttg
     1261 ctcccagtcc ccacacagcc tgagcactac cggggagtag tgccactgta cctgttgcca
     1321 gagccagccc tacagcatcc ctctcattca ttgctttctg gcaggcggca ccagaagggt
     1381 tttctgcaca tgttgccatg tcgataccat gctggcgtat ccacgcagcc tgagcatctt
     1441 cactaccgcc aagtaaatta ttctcaacag tcgtcttacc tgactgtgcg ccggctaccg
     1501 ctgaagcccc gagtcgccca ccagaccacc ggccaggcca gcagcaaccg ttgccagtgt
     1561 cgagaccgtc tgcttctctg tctcgctcag ttcactcacc ggcttaccgt acatctgtgt
     1621 ggctatcatt cccaccacct cgccggtggc tgcacctaca gcaccggcaa gagcgttatt
     1681 tccctgtgcc acagccagtg cggcattcac cacggcatga gcagccacat ttgcagcctt
     1741 attcacctca ccggtaaccg ggtctgtggt catggttttc accacttcgg ccagatacgg
     1801 tgctgcacca cctgccaggg ctgcactcag attaccgccg gccagtccct gtaccgctgc
     1861 cgttgtagcc tgtatcgttc cctccggtac caaaaactga ggccgccata tcgttgttat
     1921 acgctcgctg cgccgcatcc tgcgctgtga aggggttatc aggtccattc gagagagcca
     1981 tcttcaagca cccaaacagc cccccccgga caactctcat caaaaaacca gataccgtct
     2041 tccatctctt cttctgtttt cggaaatgcc tgtcgaaaac gctcaacctg ctcgtcaaca
     2101 cttccttcca aaaacttacc gtgcttggcg agtttgatac gcccttcttt cagaagacgg
     2161 gttaatgccc aaaaaaagat ttctcttcta tctgcaaaac taaactctgc agggtgaata
     2221 tcaggaactg aataacacaa aatagcatca agagtctgtc cctctgcagc gtctgtcaac
     2281 tccttgtatt ctctttcact taactcagta atcatttgaa cttaatctcc acacgtgaat
     2341 atttattccc cagttcagta agcttttggt tattacgaat atctatagtc caacgagcgc
     2401 ctgtctgacc ttctgatgta gatatgtttc taagtgttat taccgaacca tctgatagtt
     2461 tagacgtgta gattcccgat tttacctctt tcagcggtgt atcgcctgcc agttgctgtg
     2521 cgtaattgtg gatctgttta tccgtcaaag tctggctttc aaacactttc gtcgtaccat
     2581 aacggttact taccgaactc tgcctgatag tctgcccatc caccgttaac tctccggccg
     2641 ggtgactttt atcatatatt ttactcagtt catcaaatcg ttgttgctga acctcctgat
     2701 gacgggcttt ttcttcatta tccggtcccc atcccccccg gtattccagc acctgcacca
     2761 ccaagctcag ctttatcctt atccgttgcg ctcgctacgt ctttgcccag attcggctgt
     2821 ttgtttccgt ctgtattgtc gccattcagc acatgccccg gtcctcgggt gccatcgcga
     2881 ctcggatcac gtacagcaca ctgttcccgt agccagcgta acaccaccgg tatcccagct
     2941 attatcattc ccggtatcct tactggcatt acctgtagca ccagctattg ctgctgccgt
     3001 at
//
LOCUS       DADTFG010000264         2963 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.266, whole genome shotgun sequence.
ACCESSION   DADTFG010000264 DADTFG010000000
VERSION     DADTFG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.266"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(96..172)
                     /locus_tag="J1138_004599"
     tRNA            complement(96..172)
                     /locus_tag="J1138_004599"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(136..138),aa:Asp,seq:gtc)
     gene            complement(305..1036)
                     /gene="dnaQ"
                     /locus_tag="J1138_004600"
     CDS             complement(305..1036)
                     /gene="dnaQ"
                     /locus_tag="J1138_004600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBA8456271.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNATF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1101..1568
                     /gene="rnhA"
                     /locus_tag="J1138_004601"
     CDS             1101..1568
                     /gene="rnhA"
                     /locus_tag="J1138_004601"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBA8456272.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1565..2287)
                     /locus_tag="J1138_004602"
     CDS             complement(1565..2287)
                     /locus_tag="J1138_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA8456273.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2321..>2963
                     /gene="gloB"
                     /locus_tag="J1138_004603"
     CDS             2321..>2963
                     /gene="gloB"
                     /locus_tag="J1138_004603"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBA8456274.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQIN"
BASE COUNT          800 a          717 c          695 g          751 t
ORIGIN      
        1 caaaatcata gaccaaagaa atagcgggat ttagatgaaa aaaccgcaaa attaaaaatc
       61 ttgcggttct ctgaactcat tttcatgagt gaatttggcg gaacggacgg gactcgaacc
      121 cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct ccgcgttgtg
      181 ttccgttggg aacgaggcga atattacgga ttgcctcacc tttcgtcaac ggtttttctc
      241 atcttttgaa tcgtttgctg caaaaatcgc ccaagtcgct atttttagcg ccttttacag
      301 gtatttatgc ccgccagagg cagcttccgc ccttcttctg caccagatcg agacgggctt
      361 catgtgctgc aagctcttca tctgtcgcaa aaacaacgcg taacttactt gcctgacgca
      421 caatgcgctg aattgttgtt tcaccttgtt gctgttgtgt ctctccttcc atcgcaaaag
      481 ccatcgacgt ttgaccacct gtcatcgcca gataaacttc cgcaaggatc tgggcatcga
      541 gtaatgcccc atgcagcgtt cgtttactgt tatctatttc gtagcgggca cataacgcat
      601 cgaggctgtt gcgtttaccg ggaaacattt tcctcgccac cgcaaggcta tcggtgacct
      661 tacagaaggt attggtcttc ggaatatcgc gcttaagcaa cgaaaactcg tagtccataa
      721 agccgatatc gaacgttgcg ttatggatca ccagctccgc tccgcggata tagtccatga
      781 actcatcggc tacctcggca aacgtgggct tatcgagcaa aaattcatcg gcaataccat
      841 gtacgccaaa ggcttccgga tccaccagcc gatcgggttt gagataaaca tggaagttat
      901 tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc ttgtggcctt
      961 catagtgcgc accaatctgg ttcataccgg tggtttcggt atcgagaacg atctggcgtg
     1021 taattgcagt gctcatagcg gtcatttatg tcagacttgt cgttttacag ttcgattcaa
     1081 ttacaggaag tctaccagag atgcttaaac aggtagaaat tttcaccgat ggttcgtgtc
     1141 tgggtaatcc tggacctggg ggttacggcg ctattttacg ctatcgcgga cacgagaaaa
     1201 cctttagcgc tggctacacc cgcaccacca acaaccgtat ggagttgatg gccgctattg
     1261 tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac agccagtatg
     1321 tccgccaggg tatcacccag tggatccata actggaaaaa acgtggctgg aaaacagcag
     1381 acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgct ctggggcagc
     1441 atcaaatcaa atgggaatgg gttaaaggcc atgccggaca cccggaaaac gaacgctgtg
     1501 atgaactggc tcgtgctgcg gcaatgaatc ccacactgga agatacaggc tatcaagtcg
     1561 aagtttaagc ctgtggttta cgacattgcc gggtggcacc aaccgcctgg cgaattcgcg
     1621 gtttattctt gctctgtttc atcggattta gcgttaaagg aatagtccgt ttccgggcaa
     1681 caataagttg taagcaacca agcgcaggaa tatgcgcatt caatagtttt cctccgtgtt
     1741 tgttccacgg gagaacgtgg aaacggctgg cgtgtagcac ttcaaaattc aacaaagaga
     1801 gccagtccag ctgccgcatc agagtaaaca tccggctgtt atagggcgat gttttgcgca
     1861 ataccggcac aagtttgcgt aatcccatta aactgatggg attgaagcca ctaatcacca
     1921 gccaaccatc atcaatcagt acccgatcgg cttcacgcaa taaacgatgc ggatcagtgc
     1981 accacggcaa tgtatgtgcc agtagacaaa catcaacgga tttatcggca aaaggaagat
     2041 gaagtgggtc cgcctgcacc tggacgggca ttccttgcgc agaaacattc acttgatgag
     2101 aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta agcagatgaa
     2161 aaccatacat tttagtgaac cacgggttga gctgacgctc cagcgccttg cgataaagct
     2221 ctccccaggg caaatcgccc cagcaatcag gagccacgac agtttgaggg actcttgccg
     2281 gtttcatcac aaccttccgt ttcacactga gaggtaatct atgaatctta acagtattcc
     2341 cgcctttgat gacaattaca tctgggtttt gaatgatgaa gcaggtcgct gtctgattgt
     2401 cgatcccggg gacgcagagc cagtattaaa cgccatttcc gccaataact ggcaaccgga
     2461 ggccatattt ctcacccacc atcaccacga tcacgttggc ggcgtaaaag aactggtgga
     2521 aaagtttccg caaattgtgg tgtatggacc acaagagaca caagataagg gaacaacaca
     2581 ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat gaatttagtg taattgccac
     2641 accgggtcac actttaggac atatctgtta cttcagtaaa ccttatctat tttgcggcga
     2701 cacgctgttt tctggtgggt gtggtcggtt gtttgaaggg acaccatcac aaatgtatca
     2761 atcacttaaa aagttaagtg cgttacctga cgatacattg gtatgttgtg ctcatgaata
     2821 taccttatca aatatgaagt ttgctttgag tattcttccg cacgatttgt ccataaatga
     2881 ttattatcgt aaagttaagg agttacgggc aaaaaatcaa ataacactac ccgtaattct
     2941 gaaaaatgag cggcaaatta atg
//
LOCUS       DADTFG010000265         2873 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.267, whole genome shotgun sequence.
ACCESSION   DADTFG010000265 DADTFG010000000
VERSION     DADTFG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.267"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            188..1453
                     /locus_tag="J1138_004604"
     CDS             188..1453
                     /locus_tag="J1138_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA8456275.1"
                     /translation="MALTDAKIRAAKHTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRNITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTSVKRQHRPALDLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWAEIDIDNSMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            1709..2752
                     /locus_tag="J1138_004605"
                     /pseudo
     CDS             1709..2752
                     /locus_tag="J1138_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          798 a          681 c          655 g          739 t
ORIGIN      
        1 aacggacctc cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gcgtcctgcc
       61 gccaaatcaa ttctaccgaa ctcaaccaga ctccccccac atcaacccca ttgtgtgggt
      121 ataattgcgg gtatacccca gttcgacaga atttgtaccc tctttaacgc attcagaagg
      181 ctgaactatg gcactgactg acgcaaaaat ccgggctgca aagcacactg acaaggctta
      241 taaactcact gacggggctg gcatgttcct gctggtacat cctaatggtt cccgttactg
      301 gcgtctccgt tatcgcattc tgggtaagga gaaaactctg gcacttggtg tatacccgga
      361 ggtttctctc tccgaagctc gtacaaaacg ggatgaggcc cgaaaactga tttcggaggg
      421 gattgaccct tgcgaacaga aaagagctaa aaaagtagtc cctgatttac aactctcttt
      481 tgaacatatt gcacgacgct ggcatgccag taataaacaa tgggcacaat cacacagcga
      541 taaggtactc aaaagcctcg aaacacacgt tttccccttt atcggcaacc ggaacatcac
      601 aacactcaat accccggacc tgcttatccc tgttcgtgct gcagaagcta aacaaattta
      661 tgaaatcgcc agtcgtctgc agcaaagaat atctgctgta atgcgttatg ccgtacagtc
      721 tggcatcatc agatataatc ctgctctgga tatggctggc gcattgacct ctgtaaaacg
      781 acagcatcgc cccgctcttg atctttcacg tctgcctgaa cttctgtcgc gtattaacag
      841 ttataaaggt cagcctgtca cccggcttgc tgttatgctg aatttactgg tttttattcg
      901 ctccagtgaa ctcagatatg cccgctgggc tgaaattgat attgacaatt caatgtggac
      961 tattccagcc gaacgcaaac ctctgccagg ggtaaaattc tcacaccggg gctccaagat
     1021 gcgaacacca catcttgtgc cactcagcaa acaggctgta gccatactga cagaacttca
     1081 gacatgggct ggtgaaaatg gtctgatctt tacgggagca catgacccgc gtaaaccaat
     1141 cagtgaaaat accgtaaata aggccctcag agtgatgggg tatgacacaa ccaaggaagt
     1201 ctgtggccat ggattccggg cgatggcgtg cagtgcattg attgaatccg gtttatggtc
     1261 aagagatgcg gttgaacgtc agatgagtca tcaggagcgc aacggagtac gtgccgcgta
     1321 tattcataaa gcagaacatc tggaagaacg tcggcttatg ctgcaatggt gggcagattt
     1381 tctggatgca aacagggagg agtgtatcag tccgtttgaa tatgcaaaag ttaacaatcc
     1441 actgaaaagg tgactgaacg ccatttacac acatgctctt cctcttaagg ggaagagcac
     1501 ccgcctcaat caatttcttt tttatgccgg cggaaccatc agccaggctg aggttctcat
     1561 tcaataactc aacgtcttac cacagaacag aattcactgt attatcatgg gcaacgttaa
     1621 tcccggacaa taaaaggaca ccgatgccag gcatcacaat ttttatagtc tgcagattat
     1681 ggtccatcct tttttacagg ttcagaatat gactggcaaa ttacgctttg cagtgaacga
     1741 taaccagggc tgtttcatat ttccggaaac ctggtttggc tcgttgctgg atgagtttga
     1801 agaactgatt gatgcatacg atgcggatga aatcagcgaa acaagttaca tcaacaaact
     1861 caggcgcctt gcccgacagg agaatgactt tattgatgta cacgctcact tggcatacgt
     1921 atttctggag cagaatgccc cgcgtaaggc gctgaatgca gcccttaaag ggctggcagt
     1981 tggcaatcgt cttatccctg aaggtttcag tggcagaatc atctggatac atcctgacaa
     2041 ccgacctttt cttcgtgccc tctatgcggc aatcctggca aatgcacatt tacagcgtca
     2101 ttaggatgcc atcatgctca ttgaaaaaat actggattac aatcccgaag ataatcatgg
     2161 tgcacgctgg ctactgggac cggaactgtt acgcactggc gctcatgaac aggcacgcca
     2221 tatactgcag gaacatgcag atgaattttc accttactgg tatgaactcg gccttctgca
     2281 ttttcttaat ggggaactgg taaaagctgc aactgcattt cgtcgcggct ttgcggcaaa
     2341 tacttacatc gcagaaatac tgtgcggaaa tctgcatcca tttccacttg ctgtatggca
     2401 taacttttca ggcggtccgg atactgccga agattattat gccacatacc acccgttatg
     2461 ggggcaatat ccggaagcat tgctgtttgt aaactggctc tataatcatt cctctgtgct
     2521 gcatgaacgg gcagaaatca ttaaatgcgc cgaaatgctg atgcaggaag atgatttcga
     2581 aatatgcgaa agtattttaa gacagcagga gaagttgcgt gaaagaattg atgagacgct
     2641 ttctgagaaa attgtacaga aatgcagaaa tatgaatggt gaatatgtct ggccctggat
     2701 attgccgttt tcagcggcag gcatgaaaca tactggcata cagtatcagt agatattgca
     2761 ttagtgtatc ctgcacacaa gtaataattt atccaccaat aataacactg ttaatgtccc
     2821 cttcccctgg ttgtcagcca ggggttatct tctgaacatt tcttttgaaa agg
//
LOCUS       DADTFG010000266         2854 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.268, whole genome shotgun sequence.
ACCESSION   DADTFG010000266 DADTFG010000000
VERSION     DADTFG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.268"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            57..>2854
                     /gene="traG"
                     /locus_tag="J1138_004606"
     CDS             57..>2854
                     /gene="traG"
                     /locus_tag="J1138_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBA8456276.1"
                     /translation="MTEIYVIGGSEWLAQNLNAVAAFMQTDTWDWIRNFAVAISVLVL
                     AARYTARRDLMDILGWVFVLVFSSALLTKAQPVQIIDLSNQRSIQRVANVPLGIVLPA
                     TIITRIGYAMVLSYEMVFSQPDAVTYSKTGMLFGSSLVSRSTDFTAQNPELANLFADY
                     VQNCVVGDMLLNHKYSFEELMHSEDPYALIFRQPSPLRGVFNKDGRFQTCEEAAMPLK
                     NMLGLEAKTGSPTYSYYARRILGGRPDRDLLFGQLLGNSYQYFYQNQKTASDIIKQNI
                     VMNGLRSGIMSYSARSGDTAGMLNIASTSAIEKQRLSQATFGQVMMRSLPMLQVILIG
                     MMMGMFPILVVIGMVNQGKEVAKAYVFGWLWVMSWPLLYAILNSAMAFYGQKAGSPVV
                     LSNLSTVKLNYSDLANTAGYISGMIPFISWYIAKGLGQGLMSVSHSMTGAYQSSAASA
                     AMSVSDANYSFNNMQTDNVQGNTWDTNSSVRAGQMTAQLPTGGTQTRTGDGSSVWDAS
                     GAMSKLPVDIGVTKQIATAQQEMAREAETQAQTSMQGFNSNISSAWTQLSQFTGQRGS
                     SDSMTHGADTAQNSQSSIAAHKMASAVESYAKANNVSKEQATQELASRAVTDTAGFNT
                     RAGVSASGGLKVLGNGVTAEGYMGVSYDKRGSDTDSHSASSGTRGSQDARHDQSAQAA
                     ADFKEGMDYLTSQRTSQSGSHTDNNADSRIDQLSSSLSGAKSSYEQYSSAHTRSQELS
                     QQASRTESLSGDMRENLSQQFAQYVMDKAPNDAGNILTGQSQDAVAARQSLARDFVRE
                     QVAPQVDAQYQSSSGTLGAGMGSVGGGSSGQDIQADYNNHQSAIEKRTAQAGIRNDVG
                     AQVNGMVDNNQTAQQKIQTDIHSQQQNVEGQRNELQQNHSSARTAQNLKYSLEKEKQE
                     GIPGASSQDEMMAQAQAM"
BASE COUNT          685 a          766 c          869 g          534 t
ORIGIN      
        1 ggcacgcctg ctgacgcgtt accagaacaa ctaccagttc ggggaggcgc agtaacatga
       61 cggaaatcta tgtcatcggc ggctcggagt ggctggcgca gaaccttaac gcggtcgcgg
      121 cctttatgca gacggatacc tgggactgga tacggaattt tgcggtagcc atcagcgtgc
      181 tggtgctggc cgcgcggtat accgcccgtc gcgacctgat ggatatcctg ggctgggtgt
      241 ttgtgctggt gttctcctcg gcgttgctga ccaaggcgca gccggtgcaa atcatcgatc
      301 tgtccaatca gcgttcgatt cagcgggtgg ccaatgtgcc gctggggatt gtgctgccgg
      361 cgaccatcat cacccgcatc ggttacgcca tggtgctgag ttacgaaatg gtgttcagcc
      421 agccggatgc ggtgacctac agcaagaccg gcatgttgtt cggctcctca ctggtgtccc
      481 gaagcaccga cttcaccgcc cagaacccgg agctggccaa cctgtttgcc gactatgtac
      541 agaactgtgt ggtgggcgac atgctgctca atcacaagta cagctttgag gagctgatgc
      601 attccgaaga tccgtatgcg ctgatcttcc gccagccgag tccgcttcgc ggtgtgttca
      661 acaaggacgg ccgtttccag acctgtgaag aagccgcgat gccgctgaag aacatgctgg
      721 gcctggaagc gaaaaccggc agtccgacct acagctatta tgcgcggcgc attcttggcg
      781 gtcgtccaga ccgcgatctg ctgttcgggc agctgctggg gaacagctac cagtattttt
      841 accagaacca gaagacggcc agcgatatca tcaagcagaa catcgtcatg aacggcctgc
      901 gcagcggcat catgagctac tcggcgcgca gcggtgacac ggcggggatg ctcaacatcg
      961 ccagcacgtc ggccatcgag aagcagcgcc tgtcccaggc aacgttcggg caggtgatga
     1021 tgcgctcctt accgatgctg caggtcattc tgatcggcat gatgatgggg atgttcccca
     1081 tcctggtcgt gatcggcatg gttaaccagg gcaaggaagt ggccaaagcc tacgtgttcg
     1141 gctggctgtg ggtgatgagc tggccgctgt tgtacgccat tctcaacagc gcgatggcct
     1201 tttacggcca gaaggccggc tcgcccgtgg tgctgagcaa tctgtccacg gtgaagctga
     1261 actactccga tctggccaat accgccggct atatctccgg catgatcccg tttatctcct
     1321 ggtatatcgc caaagggctg gggcaggggc tgatgagcgt cagccacagc atgaccggcg
     1381 cctaccagag ttcggcggcg tcggcggcga tgagcgtgtc ggatgccaac tacagcttca
     1441 acaacatgca aaccgacaac gtgcagggta acacctggga taccaacagc agcgtgcgtg
     1501 ccggccagat gaccgcgcaa ctgccgaccg gtggtacaca gacccgcacg ggcgatggca
     1561 gcagcgtctg ggatgcgagc ggcgcgatgt ccaaactgcc ggtagatatc ggcgtgacga
     1621 agcagattgc gaccgcgcag caggaaatgg cgcgggaagc cgagacccag gcgcagacat
     1681 ccatgcaggg cttcaacagc aatatcagca gcgcctggac gcaactgtcg cagtttaccg
     1741 gccaacgtgg cagcagcgac agcatgaccc acggtgccga cacggcgcaa aacagccagt
     1801 ccagcatcgc ggcgcacaaa atggcgagcg cggtggaaag ttatgccaag gccaacaatg
     1861 tcagcaaaga gcaggcaacg caggagttgg cgtcgagagc cgttactgat accgcgggct
     1921 ttaatacgcg agctggtgtt tccgcaagtg gtggattgaa agtgttgggg aatggtgtaa
     1981 cggcagaagg atatatgggc gtaagttacg acaaaagagg gagcgacaca gattctcatt
     2041 ctgctagtag cggcacgcgt ggcagtcagg atgcgcgtca cgaccagagc gcccaggccg
     2101 ctgctgactt taaggaaggg atggactacc tgaccagcca gcgcaccagt cagtccggca
     2161 gtcatacgga taacaatgcg gactcgcgca ttgatcagct ttcttcgtcc ttgtcaggcg
     2221 ccaagagcag ctacgaacaa tacagtagtg cacatacccg cagccaggag ctgtcacagc
     2281 aggcttctcg taccgagagt ctcagtggcg atatgcgcga gaacctgagc cagcagtttg
     2341 cgcagtacgt gatggacaag gcaccaaacg atgccggcaa cattttgacc ggtcaatcgc
     2401 aggacgccgt tgccgcgcgt cagtcgctgg cgcgtgattt tgtgcgtgaa caggtggcgc
     2461 cgcaggtgga tgcacaatat cagtccagca gcggtacgct tggcgccggc atggggtctg
     2521 ttggtggcgg aagctccggc caggatattc aggctgatta taacaatcac cagagcgcga
     2581 ttgaaaaaag aacagcacag gccggaatac gtaatgatgt aggggctcag gttaatggta
     2641 tggttgataa taaccaaaca gcccaacaaa aaattcagac agatattcat agccaacagc
     2701 aaaatgttga agggcagaga aacgaactgc agcaaaatca ttcatcagca cgaactgcac
     2761 aaaatttaaa atatagtctt gaaaaagaga agcaggaagg tataccaggt gcaagtagtc
     2821 aagatgaaat gatggcacag gcacaagcca tgca
//
LOCUS       DADTFG010000267         2849 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.269, whole genome shotgun sequence.
ACCESSION   DADTFG010000267 DADTFG010000000
VERSION     DADTFG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.269"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            38..862
                     /gene="rhaD"
                     /locus_tag="J1138_004607"
     CDS             38..862
                     /gene="rhaD"
                     /locus_tag="J1138_004607"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBA8456277.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQILVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            872..1186
                     /gene="rhaM"
                     /locus_tag="J1138_004608"
     CDS             872..1186
                     /gene="rhaM"
                     /locus_tag="J1138_004608"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBA8456278.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKAHNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(1227..2600)
                     /locus_tag="J1138_004609"
     CDS             complement(1227..2600)
                     /locus_tag="J1138_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBA8456279.1"
                     /translation="MKKSILSLAIGLLLACSTGMAKTQHLTLEQRLEAAEMRAAKAEG
                     QVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDLKIYGDVE
                     FNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAGFSAQPLG
                     DMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTANDLYDDGSG
                     YIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRDMEQQKNV
                     AYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMSMTWNGLK
                     TDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSGVVCDNDC
                     WIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDSDDRYGAR
                     VRFKYFF"
BASE COUNT          756 a          738 c          627 g          728 t
ORIGIN      
        1 tgcatccgac aacaccgaat taacaggaac acagaacatg caaaacataa cccagtcctg
       61 gtttgtccag ggaatgatca aagccaccac cgatgcctgg ctgaaaggct gggacgagcg
      121 caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcaccat atcacgacaa
      181 ttttcaccaa caaccgcgct atatcccgct cagccagccc atgcctttac tggcaaatac
      241 accgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagcttg atcctgcggc
      301 taacttaggc atcgtaaaag tcgacagcga cggcgcgggc taccacattc tttgggggtt
      361 aaccaacgaa gccgtcccca cttccgaact tccggctcac tttctctccc actgcgagcg
      421 cattaaagcc accaatggta aagatcgggt gatcatgcac tgccacgcca ccaacctgat
      481 cgctctcacc tacgtgctgg aaaacgacac cgcggtcttc actcgccaac tgtgggaagg
      541 cagcaccgag tgtctggtgg tattcccgga tggcgttggc attctgccgt ggatggtacc
      601 cggcacggac gaaatcggcc aggcaactgc acaagagatg caaaaacatt cgctggtgtt
      661 gtggcccttc cacggtgtgt tcggttccgg accgacactg gatgaaacct tcggtttaat
      721 cgacaccgca gaaaaatcag cacaaatatt agtgaaggtt tattcgatgg gcggcatgaa
      781 acagaccatc agccgtgaag agttgatagc gctcggccag cgtttcggcg ttacaccact
      841 cgccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg tcatgcaggt
      901 aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc cggaactgga
      961 agccgtgctg aaatctcacg gtgcgcataa ctacgccatc tatctcgaca aagcgcataa
     1021 tctgctattt gccacggtag agattgaatc tgaagagcgg tggaatgcgg ttgccagtac
     1081 agatgtttgc caacgttggt ggaaatatat gaccgacgtt atgcccgcta atgcggataa
     1141 cagcccagtc agtagcgaac tgcaagaagt gttttacctg ccttaatgag ttgcggtgcc
     1201 ggaaaatatc cagcaccgcc ctttcatcag aagaagtatt taaagcgaac gcgtgcgccg
     1261 taacggtcat cactatcgtc gccgtgtatc ttatcgccgt cgctatccag aatggagtaa
     1321 tacgtgccga ggtaaatatt aaagttctcc atatccatca cattagcgaa ctgataagac
     1381 gcatgaatgg tgtgaatgtt gtatgttcct tcatcatcaa tccagcaatc gttatcacaa
     1441 accacgccac taaattcatc aatcttatta tgtgcgtaga tataacccag ctcgaaacgt
     1501 ttccacagcg cgttaatccc agcggtgaaa tctttttcat tattagcatc taaataggcg
     1561 gtattaagat taaccacgat gccattttcc ggatcggttt tcaggccatt ccaggtcatg
     1621 ctcatgccat aaccggtacg atcggactga tcgacaaaat tacccttgct atcggtataa
     1681 ccataagcat tatttaccac attcgcttcc atcgctgcgg aaacggtgaa ttcttccgtc
     1741 ggcgaccagg caattaccgg acgcagataa gcaacatttt tctgctgttc catatcgcgt
     1801 ccatgataat taccgtcgtt atataaagat gttccgtctt ccagtaacgt gtttaattca
     1861 aaataccagt tatcgagttg tttgctgacg aggaaattac cgccagcgtt agaacgcccg
     1921 cggccctctt tcatcatata gatataaccg ctgccatcgt cataaagatc gttcgcagta
     1981 ttaccggaat gttcaacaaa ggtatcctga ttcagtggga acatatcgta ggcttcaaaa
     2041 cggcctactt tgaccttcca gtcgttttct ttgccaaaga agaaaaccgc atcatcgata
     2101 tttactgagc cgtgcatatc ccccagcggt tgtgcggaga acccagcgaa atagccatta
     2161 tccagttttc gcataccatc aaaacctaac agaatacgac cattgagatt ccactgttca
     2221 ttagtgggat cgctattcac atcagcgttg gtcattgcca gcaggccatg attactttcc
     2281 gcatccatat taaattctac gtcaccgtag atttttaaat cgccataccc tgaaagcagg
     2341 agattaggcg gggtggcgtt tttcttctct gactccgtcg ttgattgacc gtttaccggc
     2401 gtgttgccct gtgcggtctt aatttcgcgg atctcggcgg cttgttgtgt ctgaagctgt
     2461 ttaacctgcc cctctgcttt tgctgcccgc atttctgccg cttccaggcg ttgttccagc
     2521 gttaaatgct gtgtttttgc cataccggta ctacatgcca ataataaacc gatggctaaa
     2581 gataatatcg attttttcat tatttacgtt ccctgaaata aaggagaccc tacgctttcc
     2641 aaaaggaata cgtatataga gaaatataag gtgtgcactt tcgaattttt aatccaactt
     2701 cagcaggccg atggatagta tcccgccgca taatatgagt aaatccgctg atttcctgga
     2761 acgtgaggaa tataacaata gtgccgtttt taactaaata tcgtaaaaaa tattagatat
     2821 gattcacgct gactcataag ccacaaata
//
LOCUS       DADTFG010000268         2826 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.270, whole genome shotgun sequence.
ACCESSION   DADTFG010000268 DADTFG010000000
VERSION     DADTFG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.270"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(37..2721)
                     /gene="chiA"
                     /locus_tag="J1138_004610"
     CDS             complement(37..2721)
                     /gene="chiA"
                     /locus_tag="J1138_004610"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HBA8456280.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVQPAPADKSN
                     DNYAVVAWKGQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRYVRAATA
                     TEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADSSNDYSL
                     QAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTATAAEMSQ
                     YGNPTTCEIDNGGVIIADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPW
                     KKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPL
                     GKTQSYSNEELNNAPQFNPETLYASDTLIRFNGESYISQSKVQKVSPSDSNPWRVFVD
                     WTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANT
                     CLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYD
                     IVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTG
                     LTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHA
                     QLKGLFGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWS
                     IARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          682 a          738 c          657 g          749 t
ORIGIN      
        1 aaaatgcggc gataccggaa ggtatcgcca acacatttat tgcttagtaa acggcgcgaa
       61 gatttcgcta aatgcgtact tcggagcctg ttctttagtc agaccgtgga attccggaga
      121 caggttagtg ccgcccggca ggtcgcgcgc gattgaccag ataccaacca taccgagatt
      181 acgcttctgc gcatcctgca tgaccagacg agcatcagag agatagaaca cctcaccctg
      241 aacgtcgttc acgccaacca tcggcgtggt acccatcatg gcgtcaattt ctgcatcact
      301 cttattgcca aagaggcctt tcagctgcgc atgcaggttg gcaatcgcag acgttgcaca
      361 cttaccgtgg atgttctggc cttcggtgtt tgcagactga caaatagcgt taccgtagtc
      421 cattgtcatc acgttcacac ccgccagctc aacacctttc gctttggcat cgctcaggac
      481 attcatccct tccggcgtca ggcctgtcgg cagaattggc aaggtatacc agatagcaat
      541 atctttgcct tctgacttcc atttatcctg cactttcttc acagcaaggt tacgacgttc
      601 aatagatgcc tgatccgcaa cccaggtgcc ttcgatatcg aagtccagga ctctgaggtt
      661 caggttatca acgatgtcat aataatgctg cattagatcg tcaacgttct tacaggaagc
      721 tgccagcggg gcattattag caccaccgat agacagcatc acatcaccgc cagcctcacg
      781 cagcgctttg attttgctgt actgagcgta attctgcata ccgtaagcgg taccccatgt
      841 cggtagacag gtgttcgcat ctttactcac cacaaacgcc agcgtgaagt ggttgacgtt
      901 atgattctta gccagcgcag ccaaatccgg gatcgtattc agcgtaaagt cgacatacgg
      961 tgcataaacg tgtttcggcc acgctttctt cggcgtacct acgcgctctt tggttccggt
     1021 ccagtcaaca aaaacacgcc acgggttgct gtcagaagga gaaactttct gcactttact
     1081 ctgagaaatg tagctctcac cgttaaagcg aatcagcgta tcactggcat aaagcgtttc
     1141 tggattaaac tgcggcgcat tattcagctc ttcgttgcta tagctctgag tcttacctaa
     1201 cggcttccac gggcgactat tgctaccggt ggcgttttgg ttcgcaacca gagcagggtt
     1261 gtcactttgc gtccagaaca gcgcttcata agcctgccca tcaacaacaa cgcgatcacc
     1321 tttcacgtac acggtggaag cggaccatgc tttcgccggt tcgtatttct tccacgggtt
     1381 atcgccgccg gggacaaagc cccatgcgtc ttgatcgaca gaggttttat aatgtgcatc
     1441 gttataatct acgatgctgt tcgcgctgta cgctttgctg gcctggaaac catccgcaat
     1501 aatgacgccg ccattatcaa tttcacaggt agtcgggtta ccgtactgac tcatttccgc
     1561 agcggtagct gtacgcacga ggcgccatgg gttattggag ttttctacac tggtgccacg
     1621 cgggcaatct tcagaaccaa cccaccaggc attctgataa acattaccat tgaagataac
     1681 atggtaaatt tcgctgcctt cctggccact ccacgcctgc agtgagtaat cgttactgct
     1741 atccgctggt gttgatggct cgttgttatc cggagttgga gtaactggag tttccggtgt
     1801 ttccggagtt ggatcaaccg gagtcacagc accgccatta ttatccggct taacggaaca
     1861 ggagcctggg ttaccatact gagtgatttc tgttgccgtt gccgcgcgaa cataacgcca
     1921 tgggttgctg gcatcgtttt ctttcgcatc gcctgggcaa tttgccgcgc ctacccacca
     1981 agcgttctta taaatgctgc cgttgtagat aacgtaccat gtagaagaac cttcctgacc
     2041 tttccaggct acgacagcat agttgtcgtt tgacttatca gctggggctg gctgaacatc
     2101 aggagccggt ttcacatccg gtgcaggctg tggtgctgaa gtacaagact gtggattact
     2161 ggtttctgag atttccgttg ccgtagcggc acgaacataa cgccatgggt tgctggcatc
     2221 attgcctttc gcatcacccg ggcagttaga agaggctacc caccaggcat ttttatatac
     2281 cgcaccatta aagacgacat accaggtttg accgccctgc tgtttattcc aggcaactac
     2341 agaagaatta gacggaacag tgccctgtgc tggtgtagcc gaaccgccat tagcaggcgt
     2401 attagatgct ggcgtatttg aagttgaaga agatgagctt ccgctctttt cgcaggaaag
     2461 tgtattgcca aactgactaa tttcagcagc tgttgcggta cgctttaaac gccacgggtt
     2521 agttgcatca tttgctttcg cttttcccgg gcaatttgta gaagaaaccc accaggcatt
     2581 ttcataaatt ttgccatcga aaataacctg atatttatta ccaccttgtt ggttattcca
     2641 tgcttccatt gccaatgctg gcagagcgga acacaccagc cccataccaa tcatagattt
     2701 agtaaatata tttaatttca ttataatccc ttgtaacgta aaaactgcaa aacaaaagtc
     2761 ctacgcagga attatttacg ttttacgaga acccttcaat atgaattaaa gtgcggcatg
     2821 aaaaat
//
LOCUS       DADTFG010000269         2812 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.271, whole genome shotgun sequence.
ACCESSION   DADTFG010000269 DADTFG010000000
VERSION     DADTFG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.271"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            74..724
                     /gene="zinT"
                     /locus_tag="J1138_004611"
     CDS             74..724
                     /gene="zinT"
                     /locus_tag="J1138_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBA8456281.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            1572..2792
                     /locus_tag="J1138_004612"
     CDS             1572..2792
                     /locus_tag="J1138_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA8456282.1"
                     /translation="MPKKAKELSGLVVSRLKSEGMYAVGGVDGLYLRIRNQSRAWVLC
                     VAMGTRINNLGRTVPRRLNMGLGPYPEVSLAEARDKARELRKQIRNGINPLQEKHEQK
                     ARQEILARKKKTFAECCEEVLEVKDSEMKNKKHLAQWRSTLETYAYPFIGKKAVSEIT
                     KVDLLAILEPIWLTKNETASRLRGRIETVIDYAKAKEYFEGDNPAAWKGMLKPLLPQP
                     SKVQIPKHHAALPYNQIGSFMKELRERSGVSPRALEFAILTAARSGEIRGAEWSEIDL
                     EGKTWTIPASRMKAAKEHRVPLSDAAVALLKALPRFKGINFVFPATRKGQLSDTALLA
                     VLKRMGYTDLTQHGFRSTFRDWAGETTNYPREVIEHALAHQLADKAEAAYQRGTLWPK
                     RVALMDEWSGYCVS"
BASE COUNT          847 a          578 c          631 g          756 t
ORIGIN      
        1 gagttgttgc attttttcat atgttacaat ataacattac acatcatgta cattaactct
       61 ggaggaaatt gttttggcga ttcgtcttca taaactggct gttgctttag gtgtctttat
      121 tgttagcgct cctgcctttt cgcatggtca tcactcacac ggcaaaccct taacagaggt
      181 cgaacaaaaa gctgctaatg gtgtttttga tgataccaat gtacaaaacc gaacgctcag
      241 tgactgggat ggagtctggc aatccgttta ccctttactg caaagtggca aacttgatcc
      301 cgtctttcag aagaaagcgg atgcagataa aaccaaaaca tttgctgaaa ttaaagatta
      361 ttatcgcaaa ggttatgcga cagatatcga aatgattggc attgaggacg gcattgtcga
      421 attccataga aataatgaaa caacatcctg taaatatgat tacgatggat acaaaatact
      481 cacctataaa tcaggcaaga aaggcgttcg ttacttattc gaatgtaaag atcctgaaag
      541 caaagcccct aaatatatac aatttagcga tcatataatt gcaccacgaa aatcatctca
      601 ttttcacatt tttatgggta atgattccca gcaatcttta ttgaatgaaa tggaaaactg
      661 gccaacgtat tatccctatc agttgagtag cgaagaagtg gtcgaggaga tgatgtctca
      721 ttgatttttt tgcgctgact tcagttattt aacagaacag tgcctggtag taacaacaat
      781 aatttaccaa gacaaataag taagacaaac tcagacacgc tcatacttga gtcgtgtctt
      841 ttttattatg tctgaagaaa aaaactgaga aaaaggcgaa caataaatac acaactcagc
      901 catacagact ggcagcgcaa atgtctccag gagcaagtca tctgtcatgg taaagctaac
      961 cagaaatata ggttaatttt ggtgtttaaa ccttttttac ttgttagtat gatacaaagg
     1021 ctttcaaaaa agctgggaaa ttcaacaact cttggaaatt atggactttg tcccgcggct
     1081 ttaatctgac ggccgcgttc cttttttggc tcaattattg accccttctc tacgcaactt
     1141 cagttcccgc cagcaacttt gcggcagttt cataggatca atgtctaaaa gaataatggt
     1201 gaccgataag aaaacgactg aataactgta gattttcgct cgaaaccttc ctgtcagacc
     1261 catagcgaat caagtgctga atgtcacagt atcgaacaga aaacagtgac gatctaaccc
     1321 ttcaagaata ttctacgatt gttctgttta ggaaaagcaa ggcgggaagt cgggagataa
     1381 gtcattgata aagtggcgga gagagggtct aaatttttag tgttcgacaa tgcccaccta
     1441 agtgctattt ttcctttaaa aacaaaatat tgtcattatt tttttgttcg gtagtgtatt
     1501 gctactgtgc aaacaatcag ctattttatg cagtaccgta tgtatggcgg gttgtatgac
     1561 aggattttgc tatgccaaag aaggccaagg aactctcagg actcgtcgtt tcacgattaa
     1621 aatccgaagg tatgtatgcc gttggtggcg tggatggttt ataccttcgc atccgaaatc
     1681 agtctcgtgc atgggttctt tgcgtggcta tgggaacccg aatcaacaat ctcggtagaa
     1741 cagttccaag acgtttgaat atgggtcttg gcccttaccc ggaagtctcc ttggccgaag
     1801 cgcgtgacaa agcgcgtgag ctacgtaaac aaatccgtaa tggtattaat ccccttcaag
     1861 aaaaacacga gcaaaaagcc cggcaggaaa tactggcccg gaagaaaaag acctttgccg
     1921 aatgttgtga agaagtgctg gaagtcaaag acagtgagat gaagaacaaa aaacatcttg
     1981 cgcaatggcg ttccacactg gaaacctatg cctacccttt cattggcaaa aaggccgtta
     2041 gtgaaatcac caaagtggat cttctggcaa tactggaacc tatctggtta accaagaatg
     2101 aaaccgccag tcgtctacgt ggacgtattg aaacggtgat cgattacgcc aaggccaaag
     2161 aatactttga aggtgataat cctgccgcat ggaaaggtat gttgaaacct ctcctgcctc
     2221 aaccaagcaa agttcagatc cccaagcacc atgcggccct tccctataac cagatcggct
     2281 cctttatgaa agaactacgt gaacgaagcg gcgtttcgcc tcgtgcgctt gagtttgcca
     2341 tactgaccgc agcacgctca ggagaaattc gtggtgccga gtggtcagag atcgatcttg
     2401 aaggtaaaac gtggaccata cctgcaagcc gaatgaaagc agcgaaggag catcgagttc
     2461 cgttgtctga tgctgctgtt gccctgttaa aggctttacc acgttttaag ggtataaatt
     2521 ttgtatttcc cgccactcgg aagggacaac tctcggatac ggctttactg gcagtcctaa
     2581 agcgaatggg atacaccgat ttaacgcagc atgggtttag atcgacattc cgtgattggg
     2641 ctggtgaaac gaccaactac ccgcgtgagg taattgaaca tgcgttagct caccaattgg
     2701 cagataaggc agaggctgcg tatcaacgtg ggacgttatg gcctaaacgg gtggcgttga
     2761 tggatgagtg gtcggggtat tgtgtttctt agacatactt gcggatgaca aa
//
LOCUS       DADTFG010000270         2754 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.272, whole genome shotgun sequence.
ACCESSION   DADTFG010000270 DADTFG010000000
VERSION     DADTFG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.272"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..294
                     /locus_tag="J1138_004613"
     CDS             <1..294
                     /locus_tag="J1138_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966178.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBA8456283.1"
                     /translation="DVPVVSAATTATGGQEAELKQKPAPGDDDAERGELPPGISGDGE
                     VVDIAQYEAWQAQQQAMGQQAMQRREETNINIDRHRRELAEVHHGDSEPGDEF"
     gene            294..>2754
                     /locus_tag="J1138_004614"
     CDS             294..>2754
                     /locus_tag="J1138_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019455149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBA8456284.1"
                     /translation="MMSIGQVGPAGKAGDYYTHQDNYYVLGSMDERWVGQGAEALGLS
                     GKVDVKDFVAVLEGKLPDGSDLTRMVGGENKHRPGYDLTFSAPKSISILAMLGGDKRL
                     IAAHNHAVEVAVREVEKLASTRVMEGGVSETKLTGNLVVALFNHDTSRDLDPQLHTHA
                     VVANVTQHDGKWQTLSSDTVGKTGFIESVYANQVAFGQIYRHIMRQEVEAMGYKTKTV
                     GPHGMWEADNMDSVVDVFSKRSHAIDAAAGPDASLKSRDMATLDTRKAKVASDPAELA
                     VVLMQEMKATGFDIQAYMAEAQSRTMVTPSPTPLEAGGDVLVDQAVGQAISALSDRQV
                     NFTHNQVANKAISYLTGEPGMLDAVRDGIDRAIEREQLIPLDKEKGVFTSDIHLLDEL
                     SVAQLSRTHLDESAVVIGAPKADPRTGSMPTYSDAVQTLAQHAPAMAVLSGIGGASVN
                     RSRVAEVVELAREQGRDATVLTVDRQSQQYLQQSVHLDEDRVVTRAALGSEALFAPNS
                     TLVVDQAEKLTLKETVALLDGAVRNNVQLVLMDTEKRKGTGNALAVMKEAGVPRYQFS
                     GRQAVPAEVVSEPDKAQRFGQLAKGVVQALVEDRPAVAQVNGPADQRMLTAMIRDELK
                     DTGLLGREETHVATLTPVWLDSKNRGAIDTYREGMVMERWDAESRQRERFVIDRVGAQ
                     TRNLVLRNSEGEKHTLAVKDVDKDWSLFRAGTMGVADGERLRVLGLEGRGKDALKAGT
                     ALTVVRAGASELQVRVAGEPDATQKPADRVLAVGSDVFSAVKVAPAYVEGLGASVSST
                     AAVYAAVKKNELNSATLSGIAR"
BASE COUNT          562 a          757 c          977 g          458 t
ORIGIN      
        1 gacgtgcctg tcgtgtcagc tgctaccacc gcgacagggg ggcaggaggc ggaactgaaa
       61 cagaaaccgg cgcccggtga tgatgatgcc gaacgggggg agttgccccc cggtatcagt
      121 ggcgacggcg aagtggtgga cattgcgcag tatgaagcct ggcaggcgca gcagcaggcc
      181 atggggcagc aagccatgca gcgccgggaa gagaccaata tcaatatcga caggcaccgc
      241 cgcgagctgg ctgaagttca tcatggggac agcgaaccgg gagatgaatt ctgatgatga
      301 gcattggcca ggtcggccct gccggtaaag ccggcgacta ctacacccat caggacaact
      361 actacgtgct ggggagtatg gacgaacgct gggtgggcca gggcgcggaa gccctgggcc
      421 tgtctggcaa ggtggacgtt aaagatttcg tagccgtgct ggaaggcaag ttgccggacg
      481 gcagtgacct gacccggatg gtcggaggcg agaacaaaca ccgtcccggc tatgacctga
      541 cgttctcggc gcccaagagc atctccattc tggctatgct gggcggcgat aaacgcctta
      601 tcgccgcgca taaccacgcg gtagaggtcg ctgtgcggga ggtggagaaa ctggcctcga
      661 cgcgggtgat ggagggcggc gtctccgaga ccaaactgac cggtaacctg gtggtggcac
      721 tctttaacca cgatacgtcg cgcgaccttg acccgcagtt gcacacccat gcggtggtag
      781 ccaacgtcac gcagcatgac ggcaagtggc agaccctgtc ctccgatacg gtcggcaaga
      841 ccgggtttat cgagagcgtg tatgccaatc aggtggcctt cggccaaata taccgccata
      901 tcatgcggca ggaagttgaa gcgatggggt acaagaccaa gacggtcggg ccgcacggta
      961 tgtgggaggc cgacaatatg gactcagtgg ttgatgtgtt ttccaagcgc agccacgcca
     1021 tcgacgccgc cgccggccct gatgcctcgc tcaagtcgcg ggatatggcg acgcttgata
     1081 cccgcaaggc caaggtcgcc agcgatccgg ccgaattagc ggttgtcttg atgcaggaaa
     1141 tgaaggccac cggttttgat attcaggctt acatggccga ggcgcagtct cgcacgatgg
     1201 tgacgccgtc cccgacgccg ctggaggcgg ggggcgatgt gctggtcgac caggcggtgg
     1261 gacaagccat ttcggcgctc tctgaccggc aggtcaactt cacgcacaac caggtggcca
     1321 acaaggccat cagctacctc accggtgaac cgggcatgct ggacgcggtg cgtgacggca
     1381 tcgaccgcgc catcgagcgc gagcagctta ttccgctcga caaggagaag ggggtattca
     1441 cctccgatat tcatctgctc gatgagctga gcgtggcgca gctgagccgt acgcaccttg
     1501 acgagagtgc ggtggtgata ggcgcgccaa aggctgatcc gcgcaccggc agcatgccaa
     1561 cctacagcga tgccgtgcag acgcttgcgc agcatgcgcc ggcgatggcg gtgttgtcgg
     1621 gcattggggg cgcatcggtg aaccgttccc gtgtggccga agtggtagaa ttggcgcgtg
     1681 agcagggccg tgatgccacc gtgctgaccg ttgaccggca gtcacagcag tatctgcagc
     1741 aaagcgtcca tctcgatgaa gaccgtgtgg tgacgcgcgc cgcgctcggc agcgaggcgc
     1801 tgtttgcgcc gaacagcacg ctggtcgttg accaggcaga gaaactgacg ctcaaggaaa
     1861 ccgtggcgct gctcgatggc gccgtgcgta acaacgtgca gctggtgctg atggataccg
     1921 aaaagcgcaa ggggacgggc aatgccctcg ccgtcatgaa ggaggccggt gtaccgcgct
     1981 atcagttcag tggccgtcag gcagtgccgg cggaagtggt gagcgagccg gacaaggccc
     2041 agcgcttcgg tcagttggcc aaaggtgtgg tgcaggcatt ggttgaagac cggccggcgg
     2101 ttgcgcaggt aaacggcccg gccgaccagc gaatgttgac ggccatgata cgtgacgagc
     2161 tgaaagatac cgggctgctg ggacgcgaag agacccatgt ggccacgctg acgccggtgt
     2221 ggctggacag caaaaatcgg ggtgccatcg atacctaccg ggaaggcatg gtgatggagc
     2281 gctgggatgc ggagagccgg cagcgcgagc ggttcgttat cgaccgggtc ggtgcgcaaa
     2341 cgcgcaacct ggtgttgcgt aactccgagg gtgaaaagca cacgcttgcc gtcaaagacg
     2401 tggacaagga ctggtcgttg ttccgggccg gtacgatggg tgttgccgac ggcgaacgcc
     2461 tgcgggtgct gggtctggaa ggacggggca aggatgcgct gaaggccggc acggcgctga
     2521 cggttgttcg tgctggcgcc agcgagctgc aggtgcgcgt ggccggcgag ccggatgcga
     2581 cgcagaagcc ggcggaccga gtgcttgcgg tcggcagcga tgtcttcagc gccgttaagg
     2641 tggcgccggc gtatgtggaa ggcctcggcg cctcggtgag cagtacggcg gcggtctatg
     2701 ccgcggtgaa gaaaaacgag cttaacagcg ccacgttgag tggcatcgcc cgta
//
LOCUS       DADTFG010000271         2667 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.273, whole genome shotgun sequence.
ACCESSION   DADTFG010000271 DADTFG010000000
VERSION     DADTFG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.273"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            238..531
                     /locus_tag="J1138_004615"
     CDS             238..531
                     /locus_tag="J1138_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019454357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBA8456285.1"
                     /translation="MAQQNVKGATTKPTAASALKNGGRSDQQVYELFLAVQRACGGRS
                     TGTSEPPSVPADAEYLVIDQGEFDSLAVWLVEGVPVAASDEMNLTHWVISAGQ"
     gene            617..>2667
                     /locus_tag="J1138_004616"
     CDS             617..>2667
                     /locus_tag="J1138_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBA8456286.1"
                     /translation="MSDIDTKAPAKPKSTAKKSPAKAASAKTAAAKFVEAALANAERK
                     NLMLKEITFTAQNARITPRTQEEIEEMATSIEATGLLQNLVVHRMADGRYGAAAGETR
                     RLGMDLLMVQGRSAAGVPVTPEFIVPVLVVDEQDAYAISVAENVRRTNLQPADQLENI
                     RLLATKGTPPEQIGAILGFSTLHVKKCLKLTTVAPALLDLLKTNQINFDQLAALGATD
                     DHLRQIQAWDKGKYDDHFRTPKALRKIVLADEVSAADSQLVNFVGIDAYQAAGGEIRE
                     EMFVDDVILTDPLKLETLAIAKLQAVADDVAKNEGWAWSVGRTREAKRYTDDEKTFEI
                     PWVTTRLTSEQKAEMDQLLAEQELLDDMLDATGLDADDEWEHAPRVEQIEARINEINA
                     AAELNNWPSEVRATAGIIAYLKDGQIQIQRGLMKVEDIKQQEKANREEIKKNTPPSEK
                     GLSQVLVASLSAERTLAVSAALAQNTNVAIALHTFTLARRVFDAGYFYVLHTSVESMR
                     SGCLSQSEEAGSVNGIANLKLNEMHESWLNQFPENWSESFEWLLAWPQGDVLRLLAYC
                     VSHGLDGRDSYLNDKRVGTKLDSVEKALDFQIGEWWKPTSDNYFSRIGKDQIVDALNS
                     AGESGKAKDAEGMKRKDAAELAAAVLSETGWLPSCMAPQPQAESENRGDAAASSANAN
                     DT"
BASE COUNT          676 a          694 c          755 g          542 t
ORIGIN      
        1 tcaaccttcc agggtgcttt agccgcagta agtcttcccg ccgcaactga taacagcaca
       61 acgcctcgct taaacaccaa ggggtgtatg aactccgcca aaaacttttt tctcccttcg
      121 gtcaaaaaaa ctttttgtct ccgccccttg gtgccgctct tcgccgtgct gaatcagttt
      181 attgcgacgg cgaatactca cagcggcatc agttatcacc ccacggagga tttcaacatg
      241 gctcaacaaa acgtcaaagg cgcgacaacg aagcctacag cagcatcagc actgaaaaac
      301 ggcggacgtt ctgaccagca ggtgtatgag ctgtttctcg ctgtgcagcg cgcctgcggt
      361 ggccgttcaa ccggcacgag tgagccgccg tccgtaccgg ctgatgctga gtatctggtc
      421 atcgatcagg gggagtttga ctctctggcc gtttggctgg tcgagggggt tccggtcgcc
      481 gccagcgatg aaatgaacct gacgcactgg gtgatcagcg cagggcagta aaaagcaggg
      541 ttgcccctgc tcctaaccgc cggtctggac gccaatctga ccggtttgac agtaataact
      601 attggagaga aatcacatgt ctgacatcga tactaaagca cctgcaaaac caaaatcaac
      661 cgccaagaaa agcccggcca aggctgcatc agccaaaacc gctgcggcga agtttgttga
      721 agctgcgctg gccaatgccg agcgtaaaaa tcttatgctc aaagagatca cctttacggc
      781 tcagaatgcg cgtattactc cccgcactca ggaagagatc gaggagatgg caaccagcat
      841 cgaggccacc ggcctgttgc agaacctcgt cgtacaccgt atggcggatg gtcgatacgg
      901 tgcggccgcc ggtgagaccc gccgccttgg catggatttg ttgatggtgc aagggcgttc
      961 agcggctggc gtaccggtaa cgccggagtt tatcgttcct gttttagtcg tcgatgaaca
     1021 ggatgcctat gcgatttctg tcgcggagaa cgtgcgccgc accaacctgc aacctgccga
     1081 tcagctcgaa aatatccgct tgttggcgac gaaaggaacg ccacctgagc aaatcggcgc
     1141 catcctcggc ttcagcaccc tgcatgttaa aaaatgcctg aaattgacta ccgtagcccc
     1201 tgcgctgctg gacctgctga aaacgaatca aatcaatttt gaccagctgg cggcgctggg
     1261 tgcgacggat gatcatctgc gccagattca ggcatgggat aaaggtaaat atgacgacca
     1321 cttccgcaca ccaaaggcgt tacgaaaaat agtgctggcc gacgaggttt cggccgcaga
     1381 cagccagttg gttaactttg ttgggattga tgcctatcaa gcagcaggcg gtgagatccg
     1441 cgaggagatg ttcgttgacg atgtcattct aaccgaccca ttgaagctgg aaacgctggc
     1501 aatcgccaag ctgcaagcgg tggcggatga tgtggcgaaa aatgaagggt gggcatggtc
     1561 ggttggccgt actcgtgagg cgaaacgtta taccgacgat gagaagacat tcgagatccc
     1621 ttgggtaacc acaaggctga ccagcgagca gaaggcagaa atggatcaac tgctcgctga
     1681 gcaagaattg ctggatgata tgctcgacgc gactggtctt gatgctgacg atgaatggga
     1741 acatgcgcca cgcgttgagc aaattgaggc acgtatcaac gaaattaacg cagctgcaga
     1801 actcaacaac tggccgtcag aagttcgtgc gacggcaggc ataatcgctt acctgaaaga
     1861 tggccagatc caaatccaac gcggcttgat gaaagtggaa gacatcaagc aacaagaaaa
     1921 agccaaccgc gaagaaatca agaagaacac cccaccgtca gaaaaagggc tttcccaagt
     1981 tttggtggca agtttgtccg ccgaacgcac tttggcagta tctgcggcgc tggcgcagaa
     2041 cactaacgtc gcgattgcac tccatacgtt cacgctggca cggcgggtgt tcgacgcggg
     2101 atatttctat gtgttgcaca cctctgtaga aagcatgcgc agcggctgct tatcccagtc
     2161 agaagaggct ggcagcgtga atggcatcgc taacctgaaa ctgaacgaga tgcatgagag
     2221 ctggttgaac caatttcctg aaaattggag cgaaagcttt gagtggctgc tggcgtggcc
     2281 acagggtgat gttctccggt tgttggcata ctgcgtttcc cacggcctcg atggccgaga
     2341 cagttatctc aatgataagc gggttggcac caagctggat tctgtagaaa aagccctcga
     2401 tttccagatc ggggaatggt ggaaaccgac gagtgacaac tatttttcgc ggattggcaa
     2461 agaccagatc gttgatgctc tcaatagcgc cggtgaaagc gggaaggcca aagatgccga
     2521 gggcatgaag cgtaaagatg ccgctgagct cgctgctgct gtgctcagcg aaaccggctg
     2581 gttgccgagc tgcatggcac cgcagccgca ggccgaatcg gaaaaccggg gggatgccgc
     2641 tgccagttcc gcaaacgcca atgatac
//
LOCUS       DADTFG010000272         2605 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.274, whole genome shotgun sequence.
ACCESSION   DADTFG010000272 DADTFG010000000
VERSION     DADTFG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.274"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(231..2498)
                     /gene="adiA"
                     /locus_tag="J1138_004617"
     CDS             complement(231..2498)
                     /gene="adiA"
                     /locus_tag="J1138_004617"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBA8456287.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESERYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKVGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          616 a          627 c          693 g          669 t
ORIGIN      
        1 acgagtttta ctaatattac aaatttttat agtaatttct catgtacata tttttttaca
       61 ggctaaaaaa aggataaata acaggcaaaa aaaagcgcgg ggtgggaccg cgcaaaaagc
      121 atgacatttt ttgtttgcag caatttttat gaaaacgttg ccatacttaa aaggcctcaa
      181 gccaggcggc gtaatgttat ttaacaatta cgccttcagc ggaatagtgg ttacgctttc
      241 acacacataa cgtggtaaat accgtcaata atttctgtcc cttctgtttc gtgttcaaat
      301 ccagggaaat gatggtccca ggattgcagt gagcgtaaat aacttacttg cggactgttt
      361 ttatcgccga agttttcacc agacagcagc atcgggattc ctggcggata cgggataact
      421 gagtttgccg cgatacgtcc tggcagattt tcaatggata ccagttcgac attgttgtcg
      481 acaatcgcgt tgtacgcttc acgcggggtg atttccgcca ccggtaggcc agaataggct
      541 tcattcaacc gtgcgccagg gttgttttct ttcagccagg caaacatggt gtcacccaga
      601 tcgtgaatcc ccatgttcgc gtaagtgtca ggatattgtt caacaagttc cggcatcacc
      661 tgcgccagcg gtgtgttggc atcatagtgg cgtttgaagg agcaaagggt gttaaccaga
      721 gtcccccatt tcccacgggt cacgcccata gagaacagga acataatctg gaagtcagtg
      781 gtacgggtag gcacaatgcc gtggcgacca agccaggctg tgaccagcgc cgccggaaca
      841 ccggtttctt ccagttcacc atcttcaccc attcccggag caaggatgct gactttaatc
      901 gggtcgagca tactccagtt atccggaata tctttgaagc cgtgccagct ttcgcccgga
      961 tgcatgaccc agcagtcctg aacggtggtc agcagtttgg ttggtgcgtc agcaaagtca
     1021 taggttttgc cggtttgtgg gtcggtgacg acttctttgt tccacggttt gaagaaccag
     1081 ctaccgtcag cggtgaactc tttatacagc cgcgccatcg cctgacggaa atcaaccgct
     1141 tcgtcgatca cttcctgggt cagtgacagg ccgctgttgc cgtccatcat cgacactgcc
     1201 acgtcgttgg atgcgcagat ggcatacagc ggggaggtgg tggcatgcat catgtaggcc
     1261 tggttgaagc gggagaagtt aatcgcccca cgaccttcac gtacatgaat ataagaggcc
     1321 tgggacagcg cattcagcag tttgtgggtg gagtgggtgg cgaaaacggt aggaccgttg
     1381 tgatcgccag gttcgccgcg catggcatag tgatcggcat agatcgggtt gaaacgtgca
     1441 tagccgtacc aggcttcgtc aaagtgcaga cgatcggagg ttttttccag cagatcctgc
     1501 gcttctttag cgttataaca cacgccgtca taggtacagt tggtcaccac gcagtaagac
     1561 ggtttttgcc cgactttgtc tttggtcagc gggctttcac tgattttctt ctgcaaggtt
     1621 tcaggttgca tttcctgcgg atagattggc ccgataatgc cgtagcggtt gcggcttggc
     1681 accatgtaga ccggtttcgc gcctgtcagc atcaagcctt gttcgatgga tttatggcag
     1741 ttacggtcaa cgaccacgac atcgttatcg gtcatgcaag cctgcatgat ggtgcggtta
     1801 gagccggaag taccgacgac taccgaccag gagcgatcgg caccaaatac gcgtgcggca
     1861 tatctttcgc tttcgccaaa tgcgccagta tggtcaagca aagaaccgag ggaagtgcgt
     1921 tcgatgccca tgtcggtgcg gaacagattt tcaccatagt agtcatggta gaaacgtccg
     1981 gcgggtgttt tggtaaaacc aacgccgccc tggtggcctg gcgctgccca ggaatattca
     2041 tggatgtcgc tatatttcat cagcgcgctg aacagtggcg gcaatagctg ctggcggtag
     2101 cgggtcatcg cggcaacggc gcgtccggcg ataaagtcgg cagtatcttc cagaatccag
     2161 gcgaattcat cgacaagctc cagcaggtcg cgatccattg cggcgagggc tttttcccga
     2221 tcgcccaaca ggaagaccgg cacgttttgt tggcgctcat gaagcttacc gatcaattgt
     2281 ctgacgtttt gatgttcgtc cggatgttcc atttgatagc tgaacatcag gcagtcaatg
     2341 gcttcgtttg aagagagaat ggcaaaacca tcatcaaagg aggtggattt aatcacggta
     2401 acattttgct ggcttaaagc atctgccaga cgctcaacgg cgttaccgac ccaggtgtct
     2461 tgatggagaa actcgctttc aacaattaat actttcatca ttgcttaccc ggttatgaag
     2521 gaatcttcgt tgcgggcaag tatcttccgg ccttttcggg ctgtaaagcg cgtgaaaaac
     2581 aaacattttt gaaatattta ttttt
//
LOCUS       DADTFG010000273         2552 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.275, whole genome shotgun sequence.
ACCESSION   DADTFG010000273 DADTFG010000000
VERSION     DADTFG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.275"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(238..465)
                     /locus_tag="J1138_004618"
     CDS             complement(238..465)
                     /locus_tag="J1138_004618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456288.1"
                     /translation="MNKSRFAFTLACFALVLLMVGLTFYPWFLSDNAAFVQTGLVLPV
                     LYLLEFGFIVPLYVAFFSKEHGMGVEKLTGG"
     gene            complement(462..2549)
                     /locus_tag="J1138_004619"
     CDS             complement(462..2549)
                     /locus_tag="J1138_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HBA8456289.1"
                     /translation="MSQLDLRLRQRVPMVHQTESSECGLACLAMICGYYGKNIDLIAL
                     RRQFNLSARGTTLAGVTAIAEQLGLSSRPLSLELDELGALKLPCILHWEFNHFVVLVS
                     INGHRAVLHDPARGRRTVSLTELSRSFTGVALEAWPGSAFTADSVRLRIHLRTLIGSV
                     HGLKGALGKIFCLSLVFETINLVMPIGTQLVMDHAIPAGDRGLLTLICVGLMLFILLR
                     AAIGMVRAWSGLVMSTLINVQWQSGLFTHLLRLPLGYFERRKLGDIQSRFGSLDTLRS
                     TFTTSIVGAIMDSIMVIGVLVMMVLYGGWLTCVVLTFTALYVLMRLLTYGYYRQLSEE
                     ALVRNARAGSYFMETLYGIATVKMQGMAERRIAHWLNLEIDTINTGIRVTKMDMLFGG
                     INTFVAACDQVVILWLGTSLVIDNQMTLGMFVAFGAFRGQFSDRIGSLTEFLLQLRMM
                     SLHNERIADIALHPREDRKPDLPYISGMQPMGLSTHALSYRYDSQSPAVFDALDINVA
                     PGESVAIVGPSGAGKTTLMKVLCGLFPPDSGKVEVNGVDIRQLGINNYHKMIACVMQD
                     DKLFSGSIRENICGFVDEVDDAWMETCARASYLHEVIMRMPMGYETLIGELGEGLSGG
                     QKQRLFIARAIYKKPAILFLDEATSALDKDSEAKVNQAVKALSITRVIIAHRETTIAS
                     ADRVIVLTHKADQ"
BASE COUNT          614 a          740 c          687 g          511 t
ORIGIN      
        1 gaatgttaag tttaatgtcc attccgtttt gaccaccctg cattatcggt actacttgcc
       61 ttggcgtatg tgtttttcac cagtggatgc tggaaaaaga acgagctaaa actcacgaag
      121 gactgataca tgttttgacg tgaaccctgt gataagtaat agctggccgt cctgagatcc
      181 ccaaaatata ggcaccaaga tattgggtca gcagaatgaa cattgtaaat gaaatcatta
      241 accgcccgtt aatttttcca cgcccatacc gtgctctttg ctaaaaaagg ccacatataa
      301 cgggacgatg aacccgaact cgaggagata cagaacgggt aacaccaggc ctgtttgcac
      361 aaacgcggcg ttatcgctca gaaaccaggg gtaaaaggtc aggcccacca tgagaagaac
      421 cagcgcaaaa caggccagcg taaatgcaaa gcgtgatttg ttcattgatc cgccttatgg
      481 gtcagaacga tgacgcgatc ggccgaggca atggtggttt cgcggtgggc aataatcacc
      541 cgtgtaatgc tgagtgcctt aacagcctga ttcaccttcg cttcactgtc cttgtccagc
      601 gcactggtcg cctcgtccag gaacaaaatc gccggtttct tataaatggc ccgggcaata
      661 aacaggcgtt gcttttgccc gccggacaat ccttccccga gttcgccgat cagggtttca
      721 taccccatcg gcatgcgcat gatcacttca tgcagatagc tagctcgggc acaggtttcc
      781 atccaggcgt catctacctc gtccacgaac ccgcaaatat tttcccggat tgagccggag
      841 aacagtttgt cgtcctgcat cacgcaggcg atcatcttgt ggtagttgtt gataccgagc
      901 tgccgaatat cgacgccgtt cacctcaacc ttccctgaat ccgggggaaa cagcccacac
      961 aacaccttca tcagcgtggt cttgccggcc ccggacggac ccacgatggc cacactttcg
     1021 cccggtgcaa cgttgatgtc caaggcatca aagaccgccg gtgactggct gtcgtaccgg
     1081 tagctcaggg catgggtgga caaccccatc ggttgcattc ccgagatata tgggaggtca
     1141 ggcttgcgat cttcccgtgg gtgcagggct atatcggcga tacgttcgtt atgcaggctc
     1201 atcatgcgca gttgcagcaa aaattccgtc agcgacccaa tacggtcaga aaactgcccc
     1261 cgaaaggcac cgaacgcgac gaacataccg agggtcatct gattatcgat caccaggctg
     1321 gtccccagcc acagtatgac gacctggtca caggccgcca cgaaggtgtt gatcccgcca
     1381 aacagcatgt ccattttggt gacccggata ccggtattga tggtgtcgat ttccaggttg
     1441 agccagtggg cgatacggcg ttccgccatg ccctgcatct tgacggtagc gataccgtac
     1501 agcgtctcca taaaatatga accggcgcgg gcatttctca ccaacgcttc ttcggacagc
     1561 tggcgataat agccgtaagt cagcaggcgc atcaacacat agagcgcggt gaaagtgagc
     1621 actacacagg tcagccagcc gccatacagc accatcatca ccagcacgcc aatcaccatg
     1681 atgctatcca tgatggcgcc gacgatactg gtggtgaagg tactgcgcag cgtatccagt
     1741 gagccgaagc gtgactgaat gtcacccagc ttgcgccgtt caaagtaacc gagcggcagg
     1801 cgcagcaggt gggtaaacag accggattgc cactgcacgt tgatgagcgt cgacatgacc
     1861 agtccggacc aggcgcgcac catgccaata gcggcccgca gcaggatgaa cagcatcagc
     1921 ccgacgcaga tgagcgtcag taacccgcga tccccggcag gaatagcgtg atccatcacc
     1981 agctgcgtgc cgatgggcat caccaggttg atggtttcga acaccagaga caggcaaaag
     2041 attttgccga gcgccccctt gagcccatgt acgctgccaa ttagcgtacg caggtgaatg
     2101 cggaggcgaa cgctgtcggc ggtgaaggcg ctgccgggcc aggcctcaag cgctacgccg
     2161 gtgaaactgc gcgacagctc tgtcaggctg acggtccggc gcccacgcgc cggatcatgc
     2221 agcaccgcac ggtgaccgtt gatactgacc agcaccacga aatggttgaa ctcccagtgc
     2281 agaatgcagg gtagtttcag cgcgccgagt tcgtccaact ccagtgacag ggggcgggag
     2341 cttaacccca gctgttcggc gatcgcggtc accccggcca acgttgtccc acgagccgac
     2401 agattgaatt gccgacgcag cgcaatcagg tcgatgtttt tgccgtagta gccgcaaatc
     2461 atcgccagac aggccagccc acattctgac gactcggtct ggtgtaccat cggaacccgc
     2521 tggcgcagcc gcagatccag ctggctgatc cg
//
LOCUS       DADTFG010000274         2531 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.276, whole genome shotgun sequence.
ACCESSION   DADTFG010000274 DADTFG010000000
VERSION     DADTFG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.276"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            79..1272
                     /locus_tag="J1138_004620"
     CDS             79..1272
                     /locus_tag="J1138_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA8456290.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRDAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(1359..2411)
                     /locus_tag="J1138_004621"
     CDS             complement(1359..2411)
                     /locus_tag="J1138_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HBA8456291.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNSLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT          726 a          588 c          622 g          595 t
ORIGIN      
        1 ttttagtaat attgatgctg gtgcgaccac tgaggaatct ttacaattca cgcccgtttt
       61 ttcttagagg agcgcaacgt ggaaaggttt gatgccatta ttataggcgc tggtgcggca
      121 gggatgttct gttctgcgtt ggcaggtcag gcaggacgcc gggttctgct gatcgataat
      181 ggtaaaaaac cagggcgcaa aatcctcatg tctggcggtg ggcgctgcaa ctttaccaac
      241 ctttatgtcg aaccaggcgc ttatctgagc cagaatccgc atttttgtaa gtctgcactc
      301 gcgcgtttta cccagtggga tttcattgat ttggtcaata aacacggcat cgcctggcac
      361 gagaaaacgt taggacagct cttctgcgat gactccgcgc agcagattgt cgacatgctg
      421 gtggatgagt gcgagaaggg caatgtggcc ttcagattgc gtagcgaagt gctgagcgtg
      481 gcgaaggatg atacaggctt cacgcttgaa ctgaacggca tgactgtcgg ttgcgaaaag
      541 ctggtcattg cgaccggtgg actgtcaatg ccggggctgg gcgcatcgcc gtttggttat
      601 aagattgccg aacaattcgg cctcaacgtg ctgccgacac gtgcgggtct ggtgccattc
      661 actctgcata aaccgttgct tgaagagtta caggtgctgg cgggcgtggc ggtgccttcc
      721 gtgattaccg ctgaaaacgg taccgttttc cgtgagaact tactcttcac ccaccgcggc
      781 ttatctggac cggcggtgtt gcagatttcc agctactggc aaccgggtga atttgtcagc
      841 atcaatctgc taccggatgt ggacctcgaa accttcctga atgagcagcg tgatgcacat
      901 ccgaatcaaa gcctgaaaaa cacgctggcg gttcatctgc cgaagcggtt ggttgaacgg
      961 ttacaacaac tcgggcaaat cccggatgtc tcgctaaaac agctcaacgt gcgtgaccaa
     1021 cagacactga ttagcacatt gactgactgg cgcgtacaac ccaacggcac tgaaggctat
     1081 cgcactgccg aagtgacgct cggcggcgtg gacaccaacg aactctcttc acggacgatg
     1141 gaagcgcgca aagtgcctgg gctgtacttc atcggcgaag tgatggacgt caccggctgg
     1201 ctggggggct ataacttcca gtgggcgtgg tcgagtgcat gggcttgtgc gcaggatttg
     1261 gtggcagaat aagaagcaaa atgtgctctc gataccagag ggattaacct gaataaaacc
     1321 agataaggcg tccaacgcct tatctgaagc cttttttatt attttttcgc tgaacccgct
     1381 tctaatatag aaacgatgga aaacagtgta aaacaaactg cacctaagcc aaccagaact
     1441 cgcgaaggaa taaagtaagc catatcggtt tgcgcaattt cagccaggaa cgcagcaagg
     1501 aacagacaaa acagacaggt aaataccgga atcatgggta ttctgttggc taacgaacat
     1561 gtgcgtcgcc ataccagcgc taacaaccac actttggaga aaatactgta gcagatcatc
     1621 cccaggcaaa tgagaataat gccgggggcc aggcgggcgc ttgcatctga actgattaat
     1681 acgtagatac caaaaataat ggtaagtgag ccaagcaaaa taacccaata gcaccacagc
     1741 caatgttctt tcactgagaa cgtattccgc gtttgatgga caatagtggc gacaaggcca
     1801 ataagacaag cgcaaattgc cgttaacccc attagtacgt gacctgcaac ataatgtggt
     1861 gtgatatctg cactacgtaa cagtatgact gaccaaatga aaccaaagag agtaagcaga
     1921 acgggaacgg caattaagct attaccgatt gttgaagaat aggcctgcaa tggcgtaccg
     1981 tcactcttac tgcccgaggc gtttttgggg atcaacagaa aatgtccgga agatgccgct
     2041 acggttgata cacaagcggc aatcatgcca acgccgaaaa taacatgacc ggcgacaaat
     2101 tcatctgcca tcacattatt gcttgccaac agtgaccatc cccaaatcat agttgtagca
     2161 gaaccggcat aaccaataac gggaaataac ctgttgtaga atttattcat gccgtgagtt
     2221 aactgagata taataataaa tgcggtagtg aacaatgcca gacatatcgc agaaagagaa
     2281 atcagaacat gccctgcaac gaaatattca ctttgccctg aaccggttaa tacaaactca
     2341 cctaatgcga tgcatattat acccatcagt aaaggaatta atttaaacaa ccatccgata
     2401 tatatattca ttttaaaccc catatatatt ttctgctaca cttcgaaaaa catatgattc
     2461 gtcttttcag ttagttaaat atgtctataa gatgccatca attaaaaaaa gtaacttcac
     2521 tataatcgga t
//
LOCUS       DADTFG010000275         2459 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.277, whole genome shotgun sequence.
ACCESSION   DADTFG010000275 DADTFG010000000
VERSION     DADTFG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.277"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            325..468
                     /locus_tag="J1138_004622"
     CDS             325..468
                     /locus_tag="J1138_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBA8456292.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            481..690
                     /gene="cspE"
                     /locus_tag="J1138_004623"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             481..690
                     /gene="cspE"
                     /locus_tag="J1138_004623"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBA8456293.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            856..1665
                     /gene="rlmA"
                     /locus_tag="J1138_004624"
     CDS             856..1665
                     /gene="rlmA"
                     /locus_tag="J1138_004624"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBA8456294.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDEKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLIELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1662..2228)
                     /gene="mntP"
                     /locus_tag="J1138_004625"
     CDS             complement(1662..2228)
                     /gene="mntP"
                     /locus_tag="J1138_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBA8456295.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          655 a          580 c          598 g          626 t
ORIGIN      
        1 tgagaaaaag tgatataact ttttattcat tgcatagcaa aaaatgtgat attgcacgca
       61 ctatgtaata acttctccca ctggcctgga acaactgaac ttattgaact atgttagaaa
      121 atacgccagt ttaagtatct gcctgaactg gcaaggttaa gcacaatgat atatcggcgc
      181 gtatttcgtt gcataagtgt gcaaaaaaag tggaagacgt atcgagattt gtgcgtctga
      241 tcgagacatg tttaaaaatg gcttgccata attaacgttg tatgtgataa cagatttcgg
      301 gttaaacgag gtacagttct gtttatgtgt ggcattttca gtaaagaagt cctgagtaaa
      361 cacgttgacg ttgaataccg cttctctgcc gagccttata ttggtgcctc atgcagtaat
      421 gtgtcagttt tatctatgtt atgcctgcgg gcgaagaaaa caatctaagg aatttttcaa
      481 atggcaaaga ttaaaggtca ggttaagtgg ttcaacgagt ctaaaggttt tggcttcatt
      541 actccggctg atggcagcaa agatgtgttc gtacacttct ccgctatcca gggtaatggt
      601 ttcaaaactc tggctgaagg tcagaacgtt gagttcgaaa ttcaggacgg tcagaaaggt
      661 ccggcagctg ttaacgtaac agctatctga tcgaatccac tgatctgaag tgtgaatacg
      721 cttcaatctc gctataaagc ctcgtcgaat gcgaggcttt ttactttgtt ttatcttcgc
      781 tccagtcgtt cggatatttg cccgccgcat gattcgcgtt acacttgcgg cctttagtat
      841 cctgccggag ttgtcatgtc gttttcctgt ccactttgcc atcagcctct ttcgcgtgaa
      901 aaaaacagct atatctgtcc ccagcgacat cagtttgata tggcgaaaga agggtatgtc
      961 aatctgctgc ccgttcagca taaacggtct cgtgatccgg gcgacagcgc ggaaatgatg
     1021 caagcacgcc gcgcattctt agatgccgga cattatcagc cgctgcgtga tgcaattgtc
     1081 ggccaactgc gggaacggct tgatgaaaag gccgcggcgg tgctggatat tggctgtggt
     1141 gaagggtatt acacacacgc atttgctgat gcgttgcccg aaatcaccac gtttggcctg
     1201 gatgtctcga aggtagcgat aaaagcggcg gcgaaacgct atccgcaggt cactttttgt
     1261 gtcgcttcca gccaccgttt gccgttctcc gataccagta tggacgccat aatacgtatt
     1321 tacgcgccgt gtaaagcaga agaattagtg cgagtagtga agcccggcgg ttgggtcatt
     1381 actgccacgc cgggaccgag acacttaatt gagctgaagg ggctgattta caatgaagta
     1441 catcttcatg cacctcatgc agaacaactg aaaggtttta cattacagca gagtgatgag
     1501 ttgtgttatc tgatgcgtct tcgcggtgat gaagccgtcg cattattgca gatgacgccg
     1561 tttgcctggc gtgcgaagcc tgaagtctgg caagcactgg cagcaaaaga agtgttcgac
     1621 tgccagacgg actttaatat tcacctctgg cagcgttctt attaaccgtg gaagtgcgtc
     1681 cagaggatct ggacgccaat accgatcagt accagcccgc cgagaatttc cgcttttttc
     1741 ccaataattg agccgataaa gcgaccaacc atcatcccta atgtcgacat aatcaaggtt
     1801 gcacaaccaa tggccaatgc ggtcgcgata atgttaacct gcaggaaagc aagaccaaca
     1861 cccacagcca tggcatccag gctggtggca atcgcggtgg ttaccagtag ccagaaaccg
     1921 tgtcgacggc gcggttcttc atcttcatca tctgcgccac gaaaaccctc aataatcatt
     1981 cgcccgccga gaaatatcag cagcacaaac gcaatccagt ggttccattc aaggacaaac
     2041 cgactggcta acatgcccat tccccagccg atcagcggcg tcagggtttc gacggcacca
     2101 aaaataaggc cggttcgcaa tgcttcagaa aatttcggtt tatggagggt ggcaccttta
     2161 ccgattgatg cagcaaaggc atccatcgac ataccaaacg caagaagaac agtagcagtg
     2221 atattcatga caacgtcctg accggggtat ccataaaaca catcatcgcc cccagcaaac
     2281 atgcatacat gaacaacaag ccgcgatggt tttcgcgttc atgcaggcat agcgattgat
     2341 gtgcatatgg tctcgcctga ctgaaagtgt tcagtccgta cgtgccacgt tttgcaacga
     2401 gtatgttgac acgtacattt ccggaatctg gaaatcagct actccccaaa tgagggcgc
//
LOCUS       DADTFG010000276         2451 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.278, whole genome shotgun sequence.
ACCESSION   DADTFG010000276 DADTFG010000000
VERSION     DADTFG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.278"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            379..756
                     /locus_tag="J1138_004626"
     CDS             379..756
                     /locus_tag="J1138_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456296.1"
                     /translation="MNTQNVNVKTAAQERSERYGEKPSALRTDGFVRNLNSSNAFDVI
                     RADVVLARMEKQAGHSCGLYYEIFESRLLEMAMNYLCELPLKDRPVFMGAASKRGFML
                     TEAEADRAYDSFISLKDELSADY"
     gene            811..1230
                     /locus_tag="J1138_004627"
     CDS             811..1230
                     /locus_tag="J1138_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TraE"
                     /protein_id="HBA8456297.1"
                     /translation="MKINNINSVVKESPDDWDVKACLFVTQEIGRFSVKFSAPCIGGT
                     VNLNCSHITNACNPFDAVARFVRETSAKGCTVYAVQQIEPVRPVCLSTTGYRFMQKGF
                     IWLCLDTGVIVEVSEDGRMRRGDDGHTLPYYGTLTRI"
     gene            1267..1614
                     /locus_tag="J1138_004628"
     CDS             1267..1614
                     /locus_tag="J1138_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020453970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456298.1"
                     /translation="MYQSEISTENRMAWDASTEAAYWQLREQEARSEVAEISLMEFMD
                     ASAVLYPKDWEGDAHSETFKLAEMYCGDVTYIFAKVGDRYFKFRDVASLSHNAILARI
                     DKEVLSRETKTQK"
     gene            1704..1967
                     /locus_tag="J1138_004629"
     CDS             1704..1967
                     /locus_tag="J1138_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456299.1"
                     /translation="MKSKDPSIMTVTDEYVKRLQAESEHMKRQRRIARGDIAAADVDP
                     DLRSLGRHIAGCVRKGKSVRVPSMRGSEWGHVLRALELTRAMA"
     gene            2186..>2451
                     /locus_tag="J1138_004630"
     CDS             2186..>2451
                     /locus_tag="J1138_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBA8456300.1"
                     /translation="MSTFRETARYHHRYEVFSDFVKLAACALENACLKSPDIEAEYMA
                     TIQRYEKADAQRMAQLLSLLVIGLDQGMGDFLGSLFMELELGSD"
BASE COUNT          563 a          638 c          728 g          522 t
ORIGIN      
        1 ctcgccgaac ccgcgtagcg gtttcgggcc tgccggctaa ggtctgaaag ccaacatcaa
       61 caacaccaag gtcaacagca tgcgcttgct gccggcgatg ccggcgccgc tttaactgtt
      121 cgtcgccagg cgacgaacgc tgaggacgcc ccggcgtttc gccgtgtcgg gtgtgttcct
      181 gctcatctca caggtggtta tctctcatcg gcccttacgc ggccgctttt gtagcaccgt
      241 cgcccggttg cccgcaaggg tgaagtgcac gccgcaagcg gcgcccttgc tgtcacccgt
      301 tcgccgtcgc tacgtcagct tttttgccgc gaaaggttcc gacgagagat aaatcaacca
      361 cccaggaagg aaagcagcat gaacacacaa aacgtcaacg tcaaaaccgc cgctcaggaa
      421 cgctctgaaa gatatgggga aaaacccagt gcgctccgta ctgatggttt tgtccgcaat
      481 ctgaactcct caaatgcctt tgatgtgatc cgcgcagatg tcgtgctggc ccggatggag
      541 aagcaggccg ggcattcatg tggcctgtat tacgaaatct ttgaatcacg gttgttagaa
      601 atggcgatga attatctgtg tgaattgcca ttgaaagacc ggccggtctt tatgggcgcg
      661 gcatcaaagc ggggttttat gctgacggag gccgaggcag accgcgccta cgattcgttc
      721 atttccctga aagatgaact gtcggcagat tactgatttt cgtcgggctg cgttctcgta
      781 gcccggaaca tcacaagagg gaaactgatt atgaaaatta acaacatcaa ttccgtagtc
      841 aaagaatcgc cagatgattg ggatgtaaaa gcctgcctgt tcgttacgca ggagatcggc
      901 cgcttttctg tcaaattctc ggcgccctgc atcgggggta cggtgaacct gaattgcagc
      961 cacatcacca acgcctgcaa tccgtttgat gccgtggcgc gatttgtgcg cgaaaccagc
     1021 gcaaagggtt gcacggtgta cgccgtgcag cagatcgagc ctgtgcgccc tgtttgcctg
     1081 agcacaaccg ggtatcgctt tatgcaaaaa ggcttcatct ggctgtgtct cgataccggc
     1141 gtgatcgtcg aagtgtcaga ggatggccgg atgcggcgcg gggacgatgg ccacacgttg
     1201 ccgtattacg gcacgttaac ccgtatttaa ccggtggcaa gccaccataa cagaggagaa
     1261 aaaacgatgt atcagagcga aatcagcacc gaaaaccgca tggcatggga tgcgagtacc
     1321 gaggccgctt attggcagct gcgagagcaa gaagcgcgct cggaagtggc ggagatcagc
     1381 ctgatggagt ttatggacgc gagcgcggtg ttgtacccga aagactggga gggcgacgca
     1441 catagcgaaa ctttcaagct ggccgaaatg tactgcgggg acgttaccta catttttgca
     1501 aaagtcggcg atcgctattt taagttccgc gatgtggcgt cgttatcaca caacgctatc
     1561 ttggcgcgga tcgataagga ggtgttaagc cgggaaacga agacccagaa atgaaaaaag
     1621 cagcgttccc gctgctctct catgccggtt tacccctgag aaagttagac cggcgttcta
     1681 tcaaactaca tggagtatcg accatgaaat cgaaagaccc aagcatcatg acagtaaccg
     1741 acgagtatgt aaagcgccta caggcagaga gcgagcacat gaagcgccag cggcggatcg
     1801 cgcgcggtga tatagccgcg gccgacgtcg atccggatct gcggtcgctc gggcgtcata
     1861 tcgccggatg tgtccgtaag ggcaaatcgg tgcgggtgcc gtcgatgcgg ggcagtgaat
     1921 gggggcacgt tctgcgtgcg ctggagttaa cgcgggcgat ggcctgagaa cgcgagccgc
     1981 cccgttttcg gggcggctat tcactgaccc ggaggtaatt atagtgtcgc aactctcttt
     2041 tgatgacttg ttttccgtac ccgtcgatgc gttatgtccg gcggagaaga acgccagggc
     2101 tgatgcggta caacaaccaa aacctgccgt actcgtaccg acccggaacc ggataatcac
     2161 cggtgacgat ccgcgtaagg catttctgtc cacgttccgc gaaacggcgc gttatcacca
     2221 tcgctatgaa gtgttcagcg attttgtgaa gttggcggcc tgcgcgttgg agaatgcctg
     2281 tttaaagtcg ccggatattg aggcggaata tatggccaca atacagcgct atgagaaggc
     2341 agacgcgcag cgcatggcac agctgctttc gttgctggtc atcgggctgg atcagggcat
     2401 gggggatttt ctcggctcgc tgtttatgga actcgagctg ggcagcgaca g
//
LOCUS       DADTFG010000277         2414 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.279, whole genome shotgun sequence.
ACCESSION   DADTFG010000277 DADTFG010000000
VERSION     DADTFG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.279"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            28..744
                     /locus_tag="J1138_004631"
     CDS             28..744
                     /locus_tag="J1138_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBA8456301.1"
                     /translation="MLSLLNFLKEETYSDFNTLRRVLGFSKNSHSPLYKVLNRAIKAG
                     FIIKHEMPGVKQKITIWGITMQGLAMVVMPDDAVFPGYFEPGKLKYWTLEHRLLNQRV
                     RIALEEKGGQGWLNGDRGEFMARYPGVRHRPDGIITLDSGAIVAVETERSMKTRARYI
                     NIINSHLAASDAGHWHYAMYVMPDDKTKTSLIRLFDSIKTVMRNNVPVPFDTRNREMF
                     LFRTIDELEQATASGRSKTC"
     gene            complement(2014..>2414)
                     /gene="clbS"
                     /locus_tag="J1138_004632"
     CDS             complement(2014..>2414)
                     /gene="clbS"
                     /locus_tag="J1138_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HBA8456302.1"
                     /translation="EGHAKGTTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEG
                     FKWNELGRLAQKFYRDYEDITEYEVLLARLKENKQQLVALIERFSNDELYGKPWYNKW
                     TRGRMIQFNTASPYKNASGRLNKLQKCLAE"
BASE COUNT          713 a          571 c          513 g          617 t
ORIGIN      
        1 gagagaaaaa aagcctctgc tgacaaaatg ctctcccttc taaattttct gaaagaagaa
       61 acttattctg attttaacac actaagacgt gttctcggct ttagcaaaaa ttctcacagc
      121 ccattatata aggtactaaa tagagcaatt aaagctggct tcatcatcaa acacgaaatg
      181 cctggggtaa aacaaaaaat aacgatttgg gggataacta tgcaggggtt agcaatggtg
      241 gtcatgcctg acgatgctgt tttccctgga tactttgaac ccggcaaact caaatactgg
      301 actctggaac atcgtctact aaatcagagg gtcagaattg cactggaaga gaaaggcggg
      361 caaggctggc ttaatggtga tcggggggag tttatggcgc gttatcctgg tgttcgccat
      421 cgccctgacg ggattatcac gttagatagc ggcgcaatcg tcgccgtcga aacggagcgc
      481 tcaatgaaaa cccgcgcacg ctatatcaac atcattaaca gtcatctggc ggccagcgat
      541 gccggacact ggcactacgc gatgtatgtc atgccggatg acaaaaccaa aacatcgtta
      601 atccggctgt ttgactccat caaaacggta atgaggaaca acgttccggt gccgttcgac
      661 acgaggaacc gcgaaatgtt tcttttccgc accattgatg aactggagca agcaaccgcc
      721 agcggtcgaa gcaaaacctg ttaaagttgg ctgtccagaa actgacggag accatagcca
      781 actaaacaga tctttattac cgtaagttag gaacattatg attagcggct aaatatcaac
      841 gcagaacaag ttgtagaatc tggatgtagt cgtcatctga tctccccccc gctgcactca
      901 ggagtggcat ttgcgacaac aagtgagtct tagaccgaca taaactgaat gaaaattcag
      961 taaaatgatg acctccagta atacaatgat gataatgcga ttatataaag gtcaaaccat
     1021 atggttgtta gcggatttca acaacggtgc tgtactcgct gagcggcaca tgaagtttga
     1081 ataatgtgga aacaacaaat ggctacatta agtcattgga catatacaga gatggttggc
     1141 aaatcgagta cacgatatgt tgcctgaaaa gtcgaccgaa cagaactata aatatcaagt
     1201 cggcataaca catctaaatt tcataaggga agatgtgcca ccggggacgt ttttttcacc
     1261 acggggactt cgggtataac gatgcaatcc gaagaaagaa atccaatgtt ggcgatattc
     1321 atcatcgctt tattcgtcca ctcatgcgaa tggcaataaa gcgactcacc aaaacagttt
     1381 ctttagcttc cctctaccaa aattccctat gatcgcatgt tagaactctg cgtgctgaac
     1441 ttcgggccat atggacggta tcattcatgc acttgctcct ttgaaattgc tggatatcat
     1501 tcgtctttat ctgcaccggc cggcacaaat agctgtaggg gaacccggcg caggcaaaaa
     1561 gagcgacttt aacgcgtttt gcaggactcc ccgttgcctt gcagggagac actgtatcat
     1621 caaacaccat cttaatccca gcacattacc ctccatcctg gctcgtacca aatcagagga
     1681 aaggcctcgc ggcaatagat tttcccagcg aagctcggag tgaaatacag tgcagcaata
     1741 tccgacatca gaatttgatc ctcctgcgtc aggatacgaa aaatcatccc gatttgagca
     1801 cccttgtcga aaaaaaatca ccttattatg aagattggtt cttaatacac atgcgttcgc
     1861 atgtgcttat gttaagtaga cttattgtct tacacatttt atccgtatca ttaaccagaa
     1921 tatgttccgg agcagcgggt gatagtgccg ccagtccaca ctcatcgctg cccggaaaaa
     1981 tcatcagtgg ggaggcaaac ggtaagcacc ccgctattct gcaagacatt tctgcagttt
     2041 atttaacctc cccgaagcat ttttataagg cgaggcggta ttaaattgaa tcatacgacc
     2101 tcgggtccat ttattatacc aaggtttacc gtaaagctcg tcgttactga atcgttcaat
     2161 caaagccacg agttgctgct tattttcctt taaccgtgcc aataaaactt cgtactctgt
     2221 aatatcctca tagtcacggt agaatttctg tgctaaacgc cccaattcat tccatttaaa
     2281 tccttcctca ggaaaaataa tagtttttcc ttttgcctct tggtcatgcc agtgtaacac
     2341 cagctctccc cagccaatca gataggaaac cagattcgct acgctaatcg tagtcccctt
     2401 cgcgtgccct tcca
//
LOCUS       DADTFG010000278         2389 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.280, whole genome shotgun sequence.
ACCESSION   DADTFG010000278 DADTFG010000000
VERSION     DADTFG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.280"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(132..767)
                     /gene="msrQ"
                     /locus_tag="J1138_004633"
     CDS             complement(132..767)
                     /gene="msrQ"
                     /locus_tag="J1138_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBA8456303.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGELGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(768..1772)
                     /gene="msrP"
                     /locus_tag="J1138_004634"
     CDS             complement(768..1772)
                     /gene="msrP"
                     /locus_tag="J1138_004634"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBA8456304.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAASFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1881..2294)
                     /gene="hiuH"
                     /locus_tag="J1138_004635"
     CDS             complement(1881..2294)
                     /gene="hiuH"
                     /locus_tag="J1138_004635"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBA8456305.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
BASE COUNT          636 a          565 c          581 g          607 t
ORIGIN      
        1 taaaataaat tattctggtg atgaaaggtt gatgacaaac gaaacataac ttgatgcgat
       61 ctgtgtgggc cggatagggc gctcgcgccg catccggccg ggataacgcg aagtttgtcg
      121 gcaatatgaa gttatagaga taatttgttg tgggcctgct tgcgtaaccg gttgaaaagg
      181 gaaagcaact tcttataccg taaggctaaa agcagtactg ccagcccagc gtagatgagt
      241 ggctgcggtg aaataatctt cactgaccac agataatgta ttggggccag gatcgcgaca
      301 agatagacga agttatgcaa ctgttgccag tgtttgccta gttttcgctg catcgactgg
      361 gttgaagtga acgctaaagc aagcaaaata acccagctga taattcctaa cgttaaataa
      421 ggccgggtaa ttaactcctt acctaataac gccagattat tcacgcccaa ctccagcaat
      481 gcgtaactgg ttaaatgcag tgtcgcccag gcaaagcacc ataagcctaa caggcggcga
      541 gtgcgtatca ataacggctg ttttgcgtag cgtgccagag gtgtgattaa caaggccgca
      601 agcaaaaatt tcagtgcagt gcgaccggta aaatgctgaa tatctttcac cggatcggca
      661 cccagttcac cgtgattgat cgcccagacc agccagagaa acggcaacaa tccggcaaga
      721 tgcaggcaaa ctttcagcca tatcacctgt tttgctgtca gacgcattta gaaattctcc
      781 cgcaaatcca ggccacgata cagcgatgcc acctggtcgg cgtaaccatt aaacagtagt
      841 gttggctggc gctgaacatc gagaatgccg cctgaaccaa taaatcgttc ggtagcctgt
      901 gaccagcgcg ggtgatcaac atgcggatta acgttggcgt aaaaaccgta ttcgtcaggc
      961 gctgccagat tccaggtggt tggcggacgc tcgcgggtca gcttaatact gacgatcgat
     1021 ttaatccctt taaagccata tttccacggc acaatcagtc gcactggcgc accattttgc
     1081 ggtggtaacg ccttgccata aacacctacg gtcatcagtg tgagcggatg cattgcttcg
     1141 tcgagacgca atccttcgac ataaggatat ttcagcccgc cgccgataaa gcggtcctgc
     1201 tggcctggca tctgttccgg cgcataaatc gtttcgaaag cgacatactt cgcattgctg
     1261 gtgggttcgg caagcgccag caatttgtgt agcggaaaac caatccacgg caccaccatc
     1321 gaccacgctt ccacgcagcg catacgataa atacgctctt ccagcgggaa gcgacgggtt
     1381 aaatcatcat gatcgagggt caatggtttt gccacttcgc cgctgatttt cagtgtccat
     1441 ggatcggttt tcaggctacc agcattagca gcgggatcgg ctttatccag cccgaattca
     1501 tagaagttgt tataaccgga gactttatct gctggcgtca gtggcaggtt attttgccag
     1561 gcggcaggct tgctgaactc cagcggtttt ccagcggggg cgggcggacg atcgttccct
     1621 ttaaaccagc taagcagatc ggcatgcgca gcgtgaggca aagaaaatga agctgcgctg
     1681 atgcccagtg cttttaacac ctgccgacgc ttcataaaga ataccgactc ggccgtaaca
     1741 tctgattctt ttaaaaattg attctttttc ataagccatc acactttttt gtcgttagcc
     1801 aacaagagcc gtttactgtt aatctgtcag ggagtcacct ccctaacagt agcagagatc
     1861 agcttttaca tatttacgca ttaactgcca cgataggttg aatacccata ttggctgagt
     1921 aacaaaggca catgataatg ctcgttcact ttattaatat gaaattcaac ggggatctca
     1981 gggaagaaac tttcaagatt ttgtttcttg aaatagtccc cggttttaaa tacgacacgg
     2041 taatcgccag tagtcgcagt ttgctcgggc cacagcgcct taattcgtcc atccttatct
     2101 gttttggcgg tattaagttg taaccagccg ttatccgctt tcttttcaag agtgactgtc
     2161 acgtcggcag cgggttttcc tgtttgctgg ttcaaaatgt gcacgctaag aatgttttgt
     2221 tgtgcggcat aaaccaaaga aggtaatgaa aatgctgccg ttgctaccga gagtactaaa
     2281 taacgcttta acatgtttat atccttgtca tgtgaatgag ttttgaggat agcgaaaaaa
     2341 cgtccagggt aaccttacag cagcattaca attttgtaat gaaccaggt
//
LOCUS       DADTFG010000279         2380 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.281, whole genome shotgun sequence.
ACCESSION   DADTFG010000279 DADTFG010000000
VERSION     DADTFG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.281"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(77..1489)
                     /locus_tag="J1138_004636"
     CDS             complement(77..1489)
                     /locus_tag="J1138_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HBA8456306.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2046..2255
                     /gene="fumD"
                     /locus_tag="J1138_004637"
     CDS             2046..2255
                     /gene="fumD"
                     /locus_tag="J1138_004637"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBA8456307.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          624 a          515 c          583 g          658 t
ORIGIN      
        1 gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat atacaaatta
       61 attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg ccgctcggta
      121 ccagtgcacc agaaaccata actacgacgt cacctttgtg tgccagaccg ctctgcagag
      181 ccagttcttt acccagacgg tagaaatcat cagtagaagt gatctcttta accagctgcg
      241 gcacaacgcc tttgctcagt accaactgat gagccgtttt ttcgttggtg gtcagtgcca
      301 ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg ccctgggtag
      361 caaccacgat cagcggggca tccagttttt cagcagtttc aacggcacca cggcataccg
      421 cttcggtaat acgcagttta cggttgtcat tgttgaactc gagacggctg ttcatcacgc
      481 ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc ttccagcggg tatttacctt
      541 ttgcggattc accagacagc atcactgcgt cagtaccgtc gaggatggcg tttgcaacgt
      601 caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc atctgggtcg
      661 cagtgataac aactttacgt gcacggatac atttttcgat catcatcttc tgggcgaaga
      721 taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg atgccgtcag
      781 aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt ttggagatga
      841 tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata acgtcagaac
      901 gcttacgaat aaaggaagca gcaacaaagt ctacgccttg ttcgcaacca aagatcaggt
      961 cctgtttgtc tttttcagcc agtgctggca gggcaatgga aacgccaggc aggttcacac
     1021 ctttgttttc gcccaggtcg ccgttgttca gcactttaca gataactttg ttaccttcaa
     1081 tggcggtaac ttccataccg atcagaccgt cgtcaaccag tacggtgttg ccaacagaca
     1141 ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg ataacagatt
     1201 tatcagtagt gaaagtaaag gtctgacccg ctttcagaga aacgtcgtta ccgccttcca
     1261 gtttcatggt gcggatttct ggacctttgg tatcaagcag gatagcggcg gttttaccag
     1321 ttttgctcat cacgttgcgc aaattctgaa tgcgctgacc gtgttctgca tagtcaccat
     1381 gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct aacatctctt
     1441 cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg acagtcttag
     1501 tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg gaaagcgaat
     1561 ccggtattac gaaagtggtt gtgcctcgct ctaaggatag gtgacatcga aaaagcgtgc
     1621 agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag tcgttgagtt
     1681 ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat acgctgaaat
     1741 taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt agacaataaa
     1801 ttgttatcaa agattcagcg ttccagggaa aaggttacgc aaaaacttcg ttgtttgcgc
     1861 aacgtctggt gccggggttg tcagtgtgag gataagtgaa agttcatccg gtgtataaat
     1921 acatcatcgc tcaattgcta cagttaaaat aatttatcac gttatttgtc tgattttgtt
     1981 tgatttttgt ttaattggtt agattgatat atgctgatag ttggcatgat aaataggtat
     2041 aaagaatggg aaaccggaca aaagaggatg aactgtaccg agaaatgtgc agagttgtcg
     2101 gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc aaagcatatt gttatcgcag
     2161 gcgtattacg tacagcatta gccaataaac gcattcagcg cagtgagctg gaaaagcagg
     2221 cgatggaaac agtcattaac gcactggtaa aatgaccatt caggatgagg cggtaagctt
     2281 catcctgata ttgttagttc agttgcggtc cagttaatca ttctaaataa caactgctga
     2341 aattatacga atttcggtgt atattttaat ttatagcgat
//
LOCUS       DADTFG010000280         2371 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.282, whole genome shotgun sequence.
ACCESSION   DADTFG010000280 DADTFG010000000
VERSION     DADTFG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.282"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            41..529
                     /gene="eco"
                     /locus_tag="J1138_004638"
     CDS             41..529
                     /gene="eco"
                     /locus_tag="J1138_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBA8456308.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(679..2325)
                     /gene="mqo"
                     /locus_tag="J1138_004639"
     CDS             complement(679..2325)
                     /gene="mqo"
                     /locus_tag="J1138_004639"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBA8456309.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLDGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          558 a          652 c          594 g          567 t
ORIGIN      
        1 catcggtgaa aacctataaa tgaagaagga aagcaaaaaa atgaagacca ttctacctgc
       61 agtattgttt gccgctttcg ccaccacttc cgcctgggcg gcagaaagcg tccagccact
      121 ggaaaaaatc gcaccttatc cacaagctga aaaaggcatg aagcgtcagg tgattcagtt
      181 aactccgcaa gaagatgaat ctaccctgaa agtagaactg ttaatcggtc agacgctgga
      241 agtcgactgc aatttgcatc gtctcggcgg gaagctggaa agcaaaacgc tggaaggctg
      301 gggctatgat tattatgtct ttgataaagt tagctcgccg gtttccacga tgatggcttg
      361 cccggatggc aagaaagaga agaaatttgt caccgcgtat ctgggcgatg ctggaatgct
      421 gcgttacaac agcaagctgc cgatcgtggt gtatacgcca gacaatgtgg atgtgaagta
      481 ccgtgtctgg aaggcggaag agaaaattga caacgcggtt gtccgctaaa ctgctgtgaa
      541 gtgctgcaac ccgtaggccg aataaggcgg ttacgccgca tccgacatct aacgcccgag
      601 ccggttgcct gatgcgacgc tgaagcgtct tatcagcata cgccacatcc ggcaaaccat
      661 gccggatgtg gcacatcatt acaacgcaat atccgccact tctttttgaa cgggttgcgg
      721 tttcaactgc ggtttcggcg tactatctgc tgcctgcggc ttgtcgtatt tcagccccag
      781 cacttcgctg gtgtactgca actcgcgttc tgttgccgct acatcaccgt tcagcttgcg
      841 tccataagac ggaacgatcg ctttcaacgt agcctgccat tgcgggctgg aaacgcgatc
      901 gccaaatact ttttccagca gattcaacat aatcggcgcg gcggttgacg ctcctggcga
      961 tgcccccaag agcgcggcaa tggtcccttg ctggtcactg acgacttcag tacccagacg
     1021 cagtacgcca cctttatcgg catcacgctt gataatctgc acgcgctgcc ccgcttgcca
     1081 caaacgccag tcctcttttt tcgcctgcgg atagtactct ttcaacgctt caaaacgatc
     1141 ctcttcactc aacatcacct gactcaccag atatttcacc agatcgaaat tatccagccc
     1201 aacgtgcatc atcggcatca cgttagaggt ggtggtggaa ctcatcagat cccataatga
     1261 accgtttttg aggaatttgg ttgagaaggt ggcaaatggc ccaaacagca ctacgcgttt
     1321 accgtcaaga acacgggtat cgatatgcgg aaccgacatc ggtggtgcgc caacggatgc
     1381 tttaccgtaa accttcgcca gatggtgatt aaccacgtcc gggttttccg aaacaaggaa
     1441 ctgtccaccc accgggaaac cggcgtagtc tttcgcctcc ggaatccccg attcctgtaa
     1501 cagtttcagc gccgcaccgc ccgcgccgat aaagacaaat ttggcgcgga tattctgtgc
     1561 agtgccgttt ttcagatcgg caacggtgac ggtccagctg ttatcgtcat tacgctttag
     1621 ggcgcggact tcgctgctga gttgcagcga gaagttagat ttcttctgta aggaggcgat
     1681 caactggcgg gtgatctcgc catagttcac atcagtgcca atttctgtgc gtgttgccgc
     1741 tactttctgt tgcggatcgc gcccttccat cactaacggt gcccactctt tgatctgcgc
     1801 gtgatcttca gagtaacgca taccgcgaaa cagcgagctt tgttgcaacg cggcgtaacg
     1861 ggcgcgcagg aaattaacgt tatcctcgcc ccagacaaag ctcatatgcg gaacggtatt
     1921 gataaatgaa cgcggagtac gcagcacacc gcgctcgacc tggtgcgccc agaactggcg
     1981 cgaaatctga aatgcttcgt taatggcgac tgctttttca atactgatgc tgccgtcggc
     2041 attttgcggg gtgtaattga gttccatcag tgcagaatgc ccggttccgg cgttgttcca
     2101 gccgttcgaa ctctcctgcg cgacaccatc caggcgctcc accatggtca tcgaccattc
     2161 aggctccagc tcgcgtaaat aggtccccaa cgtggcgctc ataatgccgc cgccaatcaa
     2221 cagtacatca gtttcctgct cctcggacgc tttcgctttt gccgccatcg aaacggcatt
     2281 aagccccacg gccatcgaga agagcatggc agtcactttt ttcatcttgt taatgcctta
     2341 cttttagtcg ctttattgca ggtgagattt g
//
LOCUS       DADTFG010000281         2368 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.283, whole genome shotgun sequence.
ACCESSION   DADTFG010000281 DADTFG010000000
VERSION     DADTFG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.283"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(396..581)
                     /locus_tag="J1138_004640"
     CDS             complement(396..581)
                     /locus_tag="J1138_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456310.1"
                     /translation="MTSNQSENEIVNTVKKYASCADDALQGGKENVKDKFRNYPLTSV
                     VVALSIGLTLGFIIGRK"
     gene            complement(1207..1296)
                     /locus_tag="J1138_004641"
     CDS             complement(1207..1296)
                     /locus_tag="J1138_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456311.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(1351..1563)
                     /gene="cspF"
                     /locus_tag="J1138_004642"
     CDS             complement(1351..1563)
                     /gene="cspF"
                     /locus_tag="J1138_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HBA8456312.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            1864..2079
                     /gene="cspB"
                     /locus_tag="J1138_004643"
     CDS             1864..2079
                     /gene="cspB"
                     /locus_tag="J1138_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HBA8456313.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAVNVIITD"
BASE COUNT          737 a          404 c          509 g          718 t
ORIGIN      
        1 atacattata tgtaagtgat tcaaatgaaa aatctcatga caggaataaa aacaatgagg
       61 atagtaaggg taaggataat aaaaaagaca accggaaaga gtaaataatc tttcctgcat
      121 aaaaatacta gggtggaata tgtcagaaca tccagatttt gatgaccgta agcctgatga
      181 tgagcccgtt gagcgtcctg atgaggggca accattgaac cctttttcac cggatgataa
      241 aagagatcct attatttaga tatagcagaa tatagccgtt taaagtaggt tgtgttttgt
      301 tgctatcctt aaaccacctc cacaggaggt ggttttttac taatggaaca ttaattggca
      361 gatgtgattg tgtacgactt atgggaggct taagattatt ttctgccaat aataaaacca
      421 agcgttaacc ctatgcttag agcaaccacc acgctagtca gtggataatt tctaaattta
      481 tctttaacgt tttctttgcc tccctgcaaa gcatcgtcag cacatgatgc atattttttg
      541 acagtattaa ctatttcgtt ttcagattga tttgaagtca tgtttgaagt cctgatgtta
      601 agtggctttt taattatagc agatatgctt tcaatgatag ggcggagagt tgttattgag
      661 gtgatactat gaccactcaa tcaacattac ataaaatgac gtttactcct gtttggcaca
      721 gtagcagaat tttgcttctg ctactgaagt cgctttagca ctagaatatg cagatggata
      781 aagcagttca gcgtatgtac tgcgtcatgc atatcggtct tgcagatatg acgagaaggg
      841 gagacaaggt gtgtgtaatg tagaagtctt taaagtgctg tctgggataa ctctatgctg
      901 aagtgcaatt gataaaagta acacgataaa ggtttttcaa tgaagatggt aacgtgatgc
      961 ttgcattttt caaaaaatat ggataaaatt ttttcaacga tgggctttgt atacccgacg
     1021 ttaagaaaaa gtagaaaacc cgctgatgag cgggttttgt gctttaaatg gggcaatggt
     1081 aatgttgaat ctcatcccgg gactcatgtc tgttaactta ttatttagct ggtgacttgg
     1141 ttatttgcct gatgtttaaa atgttttctt ccagtacaat gtccctaaac acaatgagtc
     1201 tgcttattat attattagca gagctattac ggccaaagta cagcataagc ttttaaagcc
     1261 aatcaaccag tcatcaagac agacggggtt attcataaaa actctccatg tgtgatccga
     1321 tggggcctga aattaaagct ttaatatagc tcatgaaagg taaacattgg cagctgaagg
     1381 gccacgcaga ccatttatcc ggcaaaattc cacgcgtaat ccggtggtaa tttcttctgc
     1441 atcgcggaga ttgagcgctg aaacatgaag ctggacatcg atacgaccat cggatggggt
     1501 gataagaccc ttgccgcttt tgccgtcaaa ggttttgaca attcctgtca ttttacggga
     1561 caaaaaaatt ccttaatact gataacttgg cgcactatac acacgttcct gaagaaagct
     1621 atagtttttt gatggggttg aagatggctg gatgtctaaa ataaacattg cttcatatgt
     1681 tcaactatgc gttaatgatt gcgtcggttt gaagaacaga cgatatacga agtagtttac
     1741 taaagcagtt ctcatttcag gtgttattca cttattcctt ctttgagtct ctccaattaa
     1801 gtacgaagtc gtttctgtta tgcaaaccat ttatgccgaa aggctcaagt taaggaatgt
     1861 agaatgtcaa ataaaatgac tggtttagta aaatggttta acgctgataa aggtttcggc
     1921 tttatttctc ctgttgatgg tagtaaagat gtgtttgtgc atttttctgc gattcagaat
     1981 gataattatc gaaccttatt tgaaggtcaa aaggttacct tctctataga gagtggtgct
     2041 aaaggtcctg cagcagtaaa tgtcatcatt actgattaaa attcatcgct cgtctgtata
     2101 cgataacgaa gaaggctgat gcctgagtag agatacggac agagtagtga atattggatc
     2161 tctttaataa aaagtaagga ggtccaatac atgaaacaat ggccagcata tttggcaaaa
     2221 tcttaatcag gaaaagtatg ctaaccattg tggtgaagtg caggtttgct gcatgaatag
     2281 tttcacagca gaagctaact gctggcatcg caaaacaaag tgcgtaagtg gatgactccc
     2341 acaaaaagca ccacaatctc aaacccgc
//
LOCUS       DADTFG010000282         2356 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.284, whole genome shotgun sequence.
ACCESSION   DADTFG010000282 DADTFG010000000
VERSION     DADTFG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.284"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(932..1732)
                     /locus_tag="J1138_004644"
     CDS             complement(932..1732)
                     /locus_tag="J1138_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBA8456314.1"
                     /translation="MSGLVVPEFYPAMPAQRSAPGVSLPVAIDYPAALALRQMALVHD
                     ELPKYLLAPEISALLHYVPDLHRKLLLATLWNSGARINEALALTRENFSLAPPYPFVQ
                     LATLKQRTEKATRSAGRLPAASTPPRVVPLSDPQYVAQLQMMVATLKIPLERRNARTG
                     RMEKARLWTITDRTVRSWLAEAVDAAARDGVTFSVPVTPHTFRHSYAMHMLYAGIPLK
                     VLQALMGHKSISSTEVYTKVFALDVAARHRVQFQMSEADAVAMLKDRR"
     gene            complement(1729..>2356)
                     /locus_tag="J1138_004645"
     CDS             complement(1729..>2356)
                     /locus_tag="J1138_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020453988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456315.1"
                     /translation="KIVKDSPGLWPQIITAVSTITGAVIAVSLTHRFTREREQAVAER
                     KLAGERYFIATELVFKLEDYAAACAAVAEDDGEPDERGYSRATYRVQPLEFDDVTGDW
                     RALPASVMYRVLEFHVLQPEASGAIAHAFEHDSPPDFAWGFRERRYQYARLGLRALFL
                     AKRLRKITGMPSSRLDKYDWSPQNTMWQRWRKERRFRNKLRLAERSDT"
BASE COUNT          566 a          693 c          618 g          479 t
ORIGIN      
        1 ggggatgctg cggaaaagcc tggcgaaatc atttcgcagg cgtgccgcag ttaacaagcg
       61 cagcgcgtta gtcattaaac cagtaaagga gtaggatact ccaacatagc tttctcgtca
      121 caatagaggt ttcgcatgcg aaactagttt ttccttgcag acaacgcata gctgaccatg
      181 gttagagctc cgccagtgag actgatgacg gcgaatacta tcgttatcag ggaagggcga
      241 agactatcat cgccgtacca atacagacca aaaaacaaac atgccataat cagtagcagt
      301 acagaaaaca ccgctgaatg taatctctct attagtacat cctccttgta aattccacta
      361 ccaaatgcat ttagaaaagt ttcgcatgcg aaacctgatt ttctcgacac tatcactaat
      421 tgcggaaaat tcgatacctc cgacgaaagg cgatagcaac cagctccatt cctctttttc
      481 ccgtggtgcg attagcaatt gaagccgaac gggtgcaaat cgtcccaaac catatgacca
      541 taaagagaaa cactacaatg atagcctcaa tcatctgtaa ccctcagtaa aggttgtctc
      601 tccaagtttc gcatgcgaaa ttcagctact tttatatcag tgcttactta gcgtcgacgg
      661 gagtttatcc caatgataaa cgcgacaagc gcgattccca gtgacatccc caaagcatag
      721 ccggcacgca tattaaagaa tgagtgaaac agtaaaaaga atgtaacgcc tccagcaccg
      781 gcgccaaaaa acaatttaat ataaagatcg gccgttttca tcatgaccac ctacactcct
      841 agtcaagagg atacctatcc tcaataagca ggattggaat taaaaaaatc cctctcccat
      901 tctttgtata ttcacgcaga acagccacta ttcagcgacg atctttaagc atcgccaccg
      961 cgtcggcctc cgacatctgg aattgcaccc ggtgccgcgc tgccacatcc agcgcaaaca
     1021 ccttggtata cacctccgtc gaactgatcg acttgtgccc catcagcgcc tggagcacct
     1081 tgagcggaat gccggcatac agcatgtgca ttgcgtagct gtggcggaaa gtgtgcgggg
     1141 tgacgggcac cgagaacgtc accccgtcgc gcgccgcggc atccaccgcc tccgccagcc
     1201 agctgcgcac cgtccgatcg gtgatggtcc acagccgcgc cttttccatc cggccggtgc
     1261 gcgcgttgcg gcgctcgagc ggaattttca gcgtggccac catcatctgc agctgcgcca
     1321 catactgcgg atcggacagc ggcaccacgc gcggtggtgt gctggccgcc ggcagccgac
     1381 cggcgctacg ggtggccttt tccgtgcgct gcttgagggt ggccaactgt acgaacgggt
     1441 agggcggcgc cagcgagaag ttctcgcgcg tcagcgccag tgcctcgttg atccgggcgc
     1501 cgctgttcca cagggtggcc agcagcagtt tgcggtgcaa gtccggcacg tagtggagca
     1561 gggcgctgat ctccggcgcc aacaggtatt tgggcagttc gtcatggaca agggccatct
     1621 ggcgcagcgc cagggcggcc gggtagtcga tggcgaccgg taacgaaacg cctggcgccg
     1681 atcgttgcgc gggcatcgcc gggtagaatt ccggcaccac taaaccgctc atgtatcact
     1741 cctctcggcc aatcgtagct tgttccgaaa ccgccgctct tttcgccagc gctgccacat
     1801 agtgttctgg ggtgaccagt cgtacttgtc gaggcgggaa gagggcatgc cggtgatctt
     1861 acgcaggcgc ttggccagga acagcgcccg cagaccgaga cgtgcgtact ggtatcgcct
     1921 ttcacgaaat ccccaagcaa agtcgggggg agaatcatgt tcaaaggcat gagcaatcgc
     1981 tccactggct tccggctgaa gaacatggaa ctccagcacc cggtacatga ccgatgccgg
     2041 cagcgcgcgc cagtcgccgg tcacatcatc aaattcgagc ggctgcactc ggtaggtagc
     2101 gcgagaataa ccacgctcat ctggctcacc atcatcctcg gcgaccgctg cacacgccgc
     2161 cgcatagtct tccagcttga agaccagctc cgtggcgatg aaataacgct cccccgcgag
     2221 tttccgctca gcgactgcct gctcccgctc gcgggtaaaa cggtgcgtca gactaacagc
     2281 aatcacagcg ccggtaatgg ttgaaaccgc cgtgatgatt tgcggccaca ggccggggct
     2341 gtctttgacg atcttc
//
LOCUS       DADTFG010000283         2333 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.285, whole genome shotgun sequence.
ACCESSION   DADTFG010000283 DADTFG010000000
VERSION     DADTFG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.285"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..480)
                     /locus_tag="J1138_004646"
     CDS             complement(<1..480)
                     /locus_tag="J1138_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBA8456316.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     "
     gene            complement(532..2214)
                     /locus_tag="J1138_004647"
     CDS             complement(532..2214)
                     /locus_tag="J1138_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBA8456317.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          628 a          473 c          519 g          713 t
ORIGIN      
        1 aatgacggtt aacgtattaa actctatttg atatgatttc agacgctcga tggctgctat
       61 cacttttgca aaagtaccgt tacctgaatt actgcgtctg tagcggtcat gtagctcttg
      121 ggggccatcg atcgagatac caaccagaaa ctcatgttct ttgagaaagg cacaccattc
      181 attattcaat aaaatgccat tcgtttgtaa tgcattaaaa attcgttttt ggcctgcata
      241 gcgttgttga tagtgaataa ctttacggaa aaaatccagg ccagccagag tgggttcacc
      301 gccttgccag gtaaaataga cctgattgcc agacgctgcg atatattgtt tgatgaactc
      361 tttcagagtg ctgtcatcca tccatttttc atgagtaaac tgcgactctt tttcaaggta
      421 aaaacagtaa tcacatttga gattacattg aaagctggag ggcttggctg taacgtgcat
      481 cgctatctcg ctcaataagg cggcggaaaa atccgccgca tgaaggttta gttatttcgc
      541 ttcgcttaat gctttcttga tattgttaaa cttctcctga tttacctcgc taagcggtgg
      601 ctggctgttg tcgataaact ctcttaccac gccttgcatc tctttaacga cctgcggatt
      661 ggcggcggca aggttatctt tttgctgtag atccgtcagt ttgtatagac ctaactgatt
      721 attttctact gtatagacaa gcgaataatc gttatttctc accgtataag agaattggct
      781 taagtcctcg gtgttggggt tatgcgggta atcgtctgac tgatggcgga caaatttgtg
      841 gtaattatcc cagaatggaa tattttcctc atcaaaccag tgagaataag atgttatcca
      901 ggtcagattt ttatgtggct cgccttgttt cttattttgc aaccagggca gcaaggaaac
      961 gccatccagc ttaaggtctt ttggaatgct gatatcggct gcatcaagag ctgttgggta
     1021 gaaatccatt gcggaaatca gcttgtcata attaccgggt tgaagttttc ctttccacca
     1081 cataaacatt ggggtgtgag taccgccagg ataggtctga cttttatagc ctttttgcgc
     1141 cccgttcagc ggcagaggac catcgataac tgcaccatta tcggaggtaa agagaataat
     1201 tgtattgtca tactgtccgt ttttcttcag ttgttcgaga atgcgtttta caccctgatc
     1261 aacagaataa acggaagcgt agtaattatc tgctgtttga ctaccggtat taaattgctt
     1321 ctgatattgc tccggagcag gattatcatt tggcaggtgc ggagcattat aagccaggta
     1381 aagcataaaa ggctggtcaa gtgttttggc acgatcaaca acgccaattg cctcatcggt
     1441 taactgatcg ctgatataac cttttgcggg gacgcgttca cggtttttga acagtgaagg
     1501 ggagttgtaa tatgccgttc ctgcagcatg gaatcccata aagtaatcaa agccacggtt
     1561 ttgcggttgc cattcttccg cagaaaaggt agtgaagttg tcatgatagt cacgcgtttg
     1621 tttatcttcc ggcaccggca cattactgat ttttgataag tgccatttac ctactgctgc
     1681 agtgtaataa ccatgattct ggaataattc aggcaagaaa gtttctgtta gcggaatacc
     1741 atcctgagca tcagtattgg aatagacacc aaagcgggcg ggcgctcgac cggtcattat
     1801 tgcggcgcgg gaggggccgg aaacaccgtg cgccacatag ccgttagtaa aacgtacgcc
     1861 ttcatccatt aatgaaagga gcgtcggcgt tgatttttgc gcagcttcaa tggctttatc
     1921 tatccctatt ttgtaggtat cgacaacttc acgattttcc attgtttttg ggtcaaaaga
     1981 tcccttatca aaaggaagtt gtccataacc aagatcatcc atggtcagta cgataatatt
     2041 tggctttcct ttggtactgt attcagtcgg cgtaaagtct gagaaagcaa cgtttgtttt
     2101 ggttgctttc agctttacat catctgccgc atgagcagca aatgcagcca taccagatgc
     2161 cagtatcaaa gatatcgagg tacttacgac acttttcttt aatgcagact tcataaatgt
     2221 tcctcttctt attgtactta attccaaagt aattttacga agagaaataa gttaatgtaa
     2281 gtgaagttag tcacataaat agatagcaga tttagctaaa aaaagagaaa taa
//
LOCUS       DADTFG010000284         2333 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.286, whole genome shotgun sequence.
ACCESSION   DADTFG010000284 DADTFG010000000
VERSION     DADTFG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.286"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..1083
                     /gene="ag43"
                     /locus_tag="J1138_004648"
     CDS             <1..1083
                     /gene="ag43"
                     /locus_tag="J1138_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBA8456318.1"
                     /translation="RLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDY
                     DRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGD
                     LMRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLW
                     ADIVALGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLS
                     LDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPV
                     NWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRGNITLGV
                     QAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            1204..>2333
                     /locus_tag="J1138_004649"
     CDS             1204..>2333
                     /locus_tag="J1138_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456319.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQYGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYN"
BASE COUNT          525 a          618 c          647 g          543 t
ORIGIN      
        1 aggctgcagg ccggtgcctt taactactcc ctcaaccggg acagtgatga gagctggtat
       61 ctgcgcagtg aaaatgctta tcgtgcagaa gtccccctgt atgcctccat gctgacacag
      121 gcaatggact atgaccggat tgtggcaggc tcccgcagcc atcagaccgg tgtaaatggt
      181 gaaaacaaca gcgtccgtct cagcattcag ggcggtcatc tcggtcacga taacaatggc
      241 ggtattgccc gtggggccac gccggaaagc agcggcagct atggattcgt ccgtctggag
      301 ggtgacctga tgagaacaga ggttgccggt atgtctgtga ccgcgggggt atatggtgct
      361 gctggccatt cttccgttga tgttaaggat gatgacggct cccgtgccgg cacggtccgg
      421 gatgatgccg gcagcctggg cggatacctg aatctgatac acaacgcctc cggactgtgg
      481 gctgacattg tggccctggg aacccgccac agcatgaaag cgtcaacgga caataacgac
      541 ttccgcgccc ggggttgggg ctggctgggt tcactggaaa ccggtctgcc cttcagtatc
      601 actgacaacc tgatgctgga gccacaactg cagtatacct ggcagggact ttccctggat
      661 gacggtaagg acaacgccgg ttatgtgaag ttcgggcatg gcagtgcaca acatgtgcgt
      721 gccggtttcc gtctgggcag ccacaacgat atgacctttg gcgaaggcac ctcatcccgt
      781 gcccccctgc gtgacagtgc aaaacacagt gtgagtgaat taccggtgaa ctggtgggta
      841 cagccttctg ttatccgcac cttcagctcc cggggagata tgcgtgtggg gacttccact
      901 gcaggcagcg ggatgacgtt ctctccctca cagaatggca catcactgga cctgcaggcc
      961 ggactggaag cccgtgtccg gggaaatatc accctgggcg ttcaggccgg ttatgcccac
     1021 agcgtcagcg gcagcagcgc tgaagggtat aacggtcagg ccacactgaa tgtgaccttc
     1081 tgacagaacc atcgcctctc tgtggtcccg gtcatcatga ccgggacccg gaccggcgca
     1141 acggatcttc aacgccacat tcgctggcat taacaataac atgatattca tcacggagtg
     1201 actatgttac agatagtcgg cgcgctgatc ctgctgatcg caggatttgc cattcttcgc
     1261 cttttgttca gagcattaat cagcacggct tctgcgctgg cagggctcat attgctgtgt
     1321 ctgttcggcc cggccttact ggctggctat atcaccgaac gcataacccg gttattccat
     1381 attcgctggc tggcaggcgt atttctgacg attgccggaa tgatcatcag cttcatgtgg
     1441 ggacttgatg gtaaacatat cgcgctggag gctcacacct ttgactctgt gaaatttatt
     1501 ctgaccaccg ctctcgccgg tggtctgctg gctgttcccc tgcagatcaa aaacattcag
     1561 cagtacggga tcacaccaga agatatcagc aaggaaatta acgggtatta ctgctgtttt
     1621 tatactgcct ttttccttat ggcgtgttct gcatgcgcac cattgatcgc gttacagtac
     1681 gatatttcac cgtcactgat gtggtggggc gggttgttgt actggctggc tgcattagtg
     1741 acgctgctat gggcggccag ccagatccag gcgctgaaaa aactgacctg tgccatcagc
     1801 cagacactgg aagaacaacc ggtgctcaac agtaaatcgt ggctgaccag tttgcaaaac
     1861 gattacagcc ttcctgactc actgacggag cgcatctggc tgacgctcat ttcacaacgg
     1921 atttcccggg gagagctgag ggaatttgaa ctggcagacg gaaactggtt actgaacaat
     1981 gcctggtatg aaagaaacat ggcagggttt aacgaacagt tgaaagagaa cctgtcattc
     2041 acacctgatg aactgaaaac gctcttccgg aaccgcctga atttatcacc ggaagcgaat
     2101 gacgattttc tcgatcgttg cctggacggc ggtgactggt atcccttttc agaaggtcgc
     2161 cgttttgtat cattccatca cgtggatgag cttcgtatct gtgcctcctg cgggctgaca
     2221 gaagtacatc atgccccgga aaatcataag ccggatccgg aatggtactg ctcctctctt
     2281 tgtcgcgaaa cagaaacact gtgtcaggaa atttatgaac gcccttacaa cag
//
LOCUS       DADTFG010000285         2330 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.287, whole genome shotgun sequence.
ACCESSION   DADTFG010000285 DADTFG010000000
VERSION     DADTFG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.287"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            203..1198
                     /locus_tag="J1138_004650"
     CDS             203..1198
                     /locus_tag="J1138_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBA8456320.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSYPAIHIAFGSFARRTKIRPLDDIDLMFGLSAQSATYNVLSDRITLTSSGEGS
                     RLHGYRHSGADTICSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGKGHWKFTDPRKDRDRVTTVNVQNDGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRAACSSFVDMELEALFRHLGLYVQFSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFEENKEYEKSINKWRDVFGPFFPVYG"
     gene            1202..2134
                     /locus_tag="J1138_004651"
     CDS             1202..2134
                     /locus_tag="J1138_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456321.1"
                     /translation="MNAIYTNQSTRENLDLLYAQARVYDRVKNWNRLNFIFSIIVPLL
                     LSLVTVYNRSRGFVDSELLSSLLGLYGLLVLTFNIIISGHIAALRRKAASIQEMYDCR
                     VLGIRRNDLKVEEIPRDDIIRAAAYFRDSPEKARKRFGEEGWYVSKVYDAPLAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAILVYGIAMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLGYTLRNIQDEMFAYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQE"
BASE COUNT          604 a          564 c          597 g          565 t
ORIGIN      
        1 gcattgaaaa tatttgcatt acattcaata aaagagcgag gctcttattt ccccatgtaa
       61 attttaaggc cttgtagaaa gcgagacggt agtgaacttg cggaactaag aataaactgt
      121 ctcgaccctt tcagggcact ggagtgtgca gatgtacgtc agagccctgc gccttttgca
      181 ataaataatt aaaggactga acatggcgac cacagtaata gctgccttta acgaatttat
      241 gaaagatacc gtgaatctca aaaaggcaga taccgatgac gcgcgtgcaa gtcgcgactg
      301 gcttatcggt aagatgaatg attttgagaa ggacgataaa tttccggtga gttatccagc
      361 gatccatatt gcctttggat cctttgccag aagaaccaaa atccgtcctc tggacgatat
      421 cgatctgatg ttcggcttat ccgcgcagag tgccacctat aacgttctga gtgaccggat
      481 cactctgacc tcttccggcg aggggtcgcg cctgcacggg tatcggcatt ccggagctga
      541 cactatctgc tccgtcagaa ttctgaatgc ctttaaaaac cgcctgcagg acattgccca
      601 gtacgcccag gctgatatca ggcgtaacca ggaagccgtc acgctgaaac tggtcagcaa
      661 ggactggaat tttgacatcg taccctgctt tattaccagc gaagatgcct ttggacggac
      721 ttactatctg atccccgacg gcaaaggcca ctggaagttt accgaccccc gcaaagacag
      781 ggacagagtc acaactgtca atgtgcagaa tgacggcaat gtgctgaatg tcatcagagc
      841 agtcaagtac tggcagcggc gtccgacgat gccctccatg agttcctacc tgctggagac
      901 gctgattctg gactattacg ccggcagggc agcctgctca tcgtttgtcg atatggagct
      961 ggaagcgctt ttccgccatc tcggtctgta cgtacagttc tccgtcaacg atcccaaagg
     1021 catacaggga gatatcaact ctctttcagc cgaggcgcgt aaggcgattt cggacagatg
     1081 ttatctcgat gcgcagaagg tatcggaggc gagatggttt gaggagaaca aagagtatga
     1141 aaaatccatc aataaatggc gcgatgtgtt cgggccgttc tttcctgttt acgggtgaac
     1201 catgaatgcc atttacacta accagtcgac cagagaaaac ctggatttac tgtatgcgca
     1261 ggcccgtgtt tacgacaggg taaaaaactg gaacaggctg aattttatct tcagcatcat
     1321 cgtgccgctc ctgctttcgc tcgttaccgt atacaaccgg agccggggct ttgtcgattc
     1381 agagctgctg tcttcactgc tggggctgta tggactcctg gtgctgactt ttaacattat
     1441 catcagcgga cacatcgcgg cactgcgcag gaaggcagcc tcaatccagg aaatgtacga
     1501 ctgccgggta ctgggcatcc ggcgcaatga cctgaaggtg gaggagatcc cacgggatga
     1561 catcatccgg gcagctgcct atttcaggga cagtccggaa aaagcacgaa agcgatttgg
     1621 cgaagagggg tggtacgtca gtaaagtgta tgacgcgccg ctggccgtaa tggcgctcct
     1681 gtgccacgga aaaaacctcg gctgggacaa atcgctcagg gaggtgctgc acgtatttta
     1741 tctttcagct tttattgtct cgcctgtcgc gattcttgtt tacggcatcg ccatgaaatc
     1801 cgggctgaat gagatcctct tctatgtggt gttcacactg ccggtcatcc gctatttctt
     1861 gcttcagttt ctggataatc gcagcagtat gaaaagaagc gagaagctga aaaagtacgt
     1921 tgagaaggaa ttatccggca tcagggtgtc cggaagggca gaagaagagc aactgggtta
     1981 tacgctccgg aatatccagg atgagatgtt cgcctaccgg gcctcatgtc cgcccgttcc
     2041 caacggtatt cagctgataa tgaagcccaa aaatgagcga atttatgttg attactttga
     2101 aacgaatctg aaggagctgc accttcagga gtaatccctc cttagctccg gtgtaggatt
     2161 tacgtatgcc ggagcacatc acgataaacc attcaggttg cttggctgcc agcgtaaaca
     2221 catagtcacc ttcacctcag atagttagca tccggcttta ccggttttcg gatcttgagt
     2281 gaaaaagagt gaagaccgcc aaaaatacat ccaaattact accaatcttt
//
LOCUS       DADTFG010000286         2283 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.288, whole genome shotgun sequence.
ACCESSION   DADTFG010000286 DADTFG010000000
VERSION     DADTFG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.288"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..1451)
                     /gene="pilV"
                     /locus_tag="J1138_004652"
     CDS             complement(<1..1451)
                     /gene="pilV"
                     /locus_tag="J1138_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBA8456322.1"
                     /translation="MIKKKGFTLLEVTIVLGIGTLIAFMKFQDMRNDQEAVLADNVGT
                     QIKQLGEAVNRYISIRYDKISTLSSSNNQSSDPGPRTCSAAGCEITYQTLINEGLLPV
                     GYTGTNAQKASYKIVLKRSGTAPDYVINGLITTASPWEEGGRIRYDLLGKAVQAAGVD
                     GGMSRNTKIASGYGGQWSENSNSYSNITGGGLLAYRVGYNSSMYSVYLRRDGTLPMTG
                     DLNLGGKSIKNIKDMTASGTTTTGALKTTGNASVASDLSVGGTSTLNGPVNINNNLKV
                     KSDTYLNTLSTTGLAKFGSRIATNGLNPNDLPAGWAGGVRTYDLYASGTVGVGTGKTV
                     KAYMNNAGNIYASGNLTAGTIKSNGTIESTGRIKAGEYLHLNGQATLNAKCTPNGLVG
                     RDSTGRVLSCVSGKWQTASGDGLKGIFITITDQTSGYKCVIPNSDTGACACPGSMYDS
                     RYGFLSGTLIAEYDERRCSGSKNEHCYSNFRRL"
     gene            complement(1504..2061)
                     /locus_tag="J1138_004653"
     CDS             complement(1504..2061)
                     /locus_tag="J1138_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBA8456323.1"
                     /translation="MVLLNNKRKSKKGFSLLELLLVLGIIAALVVAAFIVYPKVQASQ
                     RAQAESNNIATIQAGVKALYTSASSFTGLTNTVAVQAKIFPDNMLSGSGTAAKPINAF
                     KGNVMLAATATGPSSATGSSFTITYDNVPAAECVKIATAAAGNFYITTVGTKVVKAAG
                     GTLDVAATAAACTNATSNTLVFTSI"
BASE COUNT          613 a          562 c          419 g          689 t
ORIGIN      
        1 tataaccttc taaaattact ataacagtgc tcgttcttac taccactaca acgacgttca
       61 tcatattccg caataagtgt tccagataaa aaaccatacc ttgagtcata catcgacccc
      121 ggacaagcac atgcacctgt atcactattt ggtattacac atttatatcc agatgtttga
      181 tcggttattg ttataaaaat acctttcagg ccatcaccgc ttgctgtctg ccatttacca
      241 ctgacacacg ataacactcg acctgtactg tctcgcccca ccagaccatt aggcgtacat
      301 ttcgcattta atgttgcctg accattcaaa tggagatatt cacctgcttt gattctgcct
      361 gttgattcaa ttgttccatt ggattttatc gttccagcag taagattacc tgacgcataa
      421 atattacccg cattgttcat gtaagcttta actgttttac ctgttcctac accaacagtc
      481 cctgacgcat acaaatcata ggtgcgaaca ccaccagccc acccagcagg cagatcgttt
      541 ggattaagtc cattcgtcgc gatacgacta ccaaacttcg ccagacctgt tgttgatagc
      601 gtgttcagat aggtgtcgga ttttactttc aggttgttgt tgatatttac aggaccatta
      661 agggtagatg ttccacccac actcagatct gatgcaacag acgcattacc cgttgtctta
      721 agtgctccgg ttgttgttgt ccctgatgcc gtcatatctt tgatgttttt gatgctcttc
      781 ccaccaagat tcagatcgcc cgtcatcggc aacgtcccat cacgacgcag ataaacagaa
      841 tacattgaag agttataccc cacgcggtaa gccagtaacc caccccccgt gatattgctg
      901 tagctattag agttctcact ccactgacca ccatagccgg acgctatctt ggtgtttctg
      961 ctcatcccac catcaacacc tgccgcctga accgcttttc ctaacaggtc ataacgaata
     1021 cgaccacctt cctcccacgg gctggcggtt gttataaggc cattaatgac ataatcaggc
     1081 gctgttcctg aacgctttaa cacaatcttg taggatgctt tttgcgcgtt cgttcctgta
     1141 tagcctacag gtaacagacc ttcattaatt aatgtctggt aagtgatttc acaaccagca
     1201 gccgaacagg ttcttggccc cggatcgcta ctttgattgt tggatgatga cagtgtggaa
     1261 atcttgtcat aacgaatact gatatagcgg ttaaccgctt caccaagctg tttaatctgt
     1321 gttccgacat tatcagccaa taccgcttcc tgatcgtttc tcatatcctg aaacttcata
     1381 aaagcgataa gcgttccaat acctaacaca atcgtcactt ccagtaaggt aaatcctttt
     1441 ttctttatca taattatctc cctgttttag caataagaaa gaaaagggcc tgcgccctct
     1501 tcattaaata gacgtgaaaa ctaatgtatt acttgtggca ttcgtacatg ctgctgccgt
     1561 agctgccaca tcgagtgttc ctcctgcggc tttaacaacc ttggttccaa ccgtagtaat
     1621 gtagaaatta cctgccgccg ctgtcgcaat cttcacacat tccgctgctg gcacattatc
     1681 gtatgtgata gtgaaagaag agccagtcgc tgatgacgga cctgtcgctg tagcagccag
     1741 catcacatta cctttaaagg cattgatggg tttagcagcc gttcctgacc cacttaacat
     1801 attgtcaggg aagatttttg cctgaacagc cacagtattt gtcaggccag taaagcttga
     1861 tgccgacgtg tataacgctt taacacctgc ctgtatggtg gcaatgttat tggattctgc
     1921 ctgcgctctt tgtgaagcct gaaccttggg ataaacgata aaagcagcta ccactaatgc
     1981 agcaatgatg cccaaaacaa gtaatagttc gagtagtgag aaacctttct tgctttttct
     2041 tttgttattt aataaaacca ttattatctc cttttttgtt ttaatgataa ctcctgttat
     2101 aacgcttctc tgatatctgg caatagaaag agcaagatat atttaactct gtctgacaga
     2161 cggatgaatt aaaaaaggca aagatttcgc tttgccttcc gggagattta aaaacaatat
     2221 ttgttatcac tactactgtc attactactt cctcatggga tatttatcat atctgataaa
     2281 aaa
//
LOCUS       DADTFG010000287         2280 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.289, whole genome shotgun sequence.
ACCESSION   DADTFG010000287 DADTFG010000000
VERSION     DADTFG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.289"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..656)
                     /locus_tag="J1138_004654"
     CDS             complement(<1..656)
                     /locus_tag="J1138_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019455152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-pilin acetylation protein TraX"
                     /protein_id="HBA8456324.1"
                     /translation="MAMFDPHSGKLQQVREVVRFTPLQQDVLKFLALVLMVFDHANRA
                     LGLHEPVLLLLGRGAFPLFALLWGYNLASRPVRQAQATRLWIWAVVAQPGYWLVMRHD
                     GLAFTDPNILFLFAAVTQALAVASLPMRKGAPNPVLAGGVLLALWWAYGTTDGSYGTA
                     GVLLLLASYGLCVSLPGYVTAVWPVRGLSIVAWPLLVLMLNAELGGTMMLAGLLLPAL
                     L"
     gene            complement(670..1194)
                     /locus_tag="J1138_004655"
     CDS             complement(670..1194)
                     /locus_tag="J1138_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019455151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBA8456325.1"
                     /translation="MFGVAALVLSGWLPASPLLAAQAVQVGFSPEGSAQQLVLNVIGE
                     ARRDIRLMGYAFTSPEVARALIAAHRRGVAVWVVVDAKANQNGAGRAALNLLANAGVN
                     VRTVNRYKILHDKVIVVDGVTTETGSFNFSRAAHRANSENVVVLRDMPEVAKQYLAHW
                     QSRWAQGQDWQSTY"
     gene            complement(1217..1435)
                     /locus_tag="J1138_004656"
     CDS             complement(1217..1435)
                     /locus_tag="J1138_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019455150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456326.1"
                     /translation="MNTTQNVSTTQGVLLTTAILLSGAATLWGYVQWDVLSDLPLYWR
                     NALVPLPLLVFMGMKHLQAQLNRADKHE"
     gene            complement(1514..>2280)
                     /locus_tag="J1138_004657"
     CDS             complement(1514..>2280)
                     /locus_tag="J1138_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019455149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBA8456327.1"
                     /translation="LAHYVSPGKKYPQPHAMLPMYDANGKPAGAWLAPLADTQGKSVG
                     GLPTQGRVFGSDSAQFVALQGSRNNETRLAGTMQDGIRLAARYPDSGVVVRLQGDDVP
                     FNPQAMTGGRVWVNDETLKATARESGASDGVIKLPESPEERLQRELQTQADKLGREKT
                     TPDPQRLAGLNDEQLRALFARGELRAEDVKARLPAAVMREVMGQDAALRVDAAVRQVA
                     REPVSTRSRLQQDEQNLVRDTHPERELNKEKTLGGD"
BASE COUNT          410 a          832 c          644 g          394 t
ORIGIN      
        1 agcagtgccg gcagcagcaa cccggccagc atcatcgtgc cgcccagctc ggcattgagc
       61 atcagcacca gtagcggcca ggccacaatc gacagcccgc gcaccggcca aacggccgtc
      121 acgtaccccg gcagagaaac acacagccca taactggcca acagcaacag aacgccggca
      181 gtaccgtagc tgccgtctgt ggtgccatac gcccaccaca gcgccagcaa aacgccgccg
      241 gccaataccg ggttaggcgc gcccttgcgc atcggcaacg acgccaccgc cagcgcctgc
      301 gtcaccgcgg cgaacaggaa caaaatattc ggatcggtga acgccagccc gtcatgacgc
      361 attaccagcc aatagcccgg ctgcgcaacc acggcccata tccacagccg ggtcgcctgc
      421 gcctgccgga caggccgcga cgccaggttg tacccccaca gcagggcgaa cagcggaaac
      481 gctccccgcc ccagcagcaa cagcaccggc tcatgcagcc ccagcgcccg gttggcgtgg
      541 tcgaacacca tcagcaccag cgccaaaaat ttcagtacgt cctgctgcaa cggggtgaaa
      601 cgcaccacct cgcgcacctg ctgcagtttt ccgctgtgcg gatcaaacat cgccatccga
      661 tttcccttct tagtaagtcg actgccagtc ctggccctgc gcccagcggg attgccagtg
      721 cgccagatac tgtttggcca cctccggcat gtcgcgcagg accaccacat tttccgagtt
      781 ggcgcggtgt gccgctcggc tgaaattgaa gctgcccgtt tcggtcgtca cgccatccac
      841 gacgatcacc ttgtcgtgca aaatcttata gcggttgacc gtcctgacgt tcacgccggc
      901 gttggccagc aggttcagtg ccgcccggcc ggcgccgttc tggttggcct tcgcatcgac
      961 caccacccag acagccaccc cgcgccggtg cgccgcaatc aacgcccgcg ccacctccgg
     1021 cgaggtaaag gcgtatccca tcaggcgaat atcgcgccgc gcttcaccga taacgttcaa
     1081 taccaactgc tgcgccgacc cttccggcga aaaccctacc tgcaccgcct gcgccgccaa
     1141 caggggcgat gccggcagcc agccgctgag caccagagcg gcaacgccaa acacgcgggc
     1201 gggcagtcgc ggtgccttac tcatgcttgt ccgccctgtt caactgcgcc tgcaggtgct
     1261 tcatgcccat gaagaccagc agcggcagcg ggacgagcgc attgcgccaa tacagcggca
     1321 gatcgctcag cacgtcccac tgcacatatc cccagagcgt cgcggctccg gacagcaaga
     1381 tcgcagtggt taacaagacg ccctgcgtcg ttgatacgtt ttgagttgtg ttcatccgtt
     1441 cttcctttag gtaatgccgt gcgggacagg ggctcgtcac ctgtctcgca cggcgttgga
     1501 caagcgcagc gcgtcagtcg ccgcccaggg ttttctcttt gttcagttcc cgttcaggat
     1561 gggtatcgcg caccagattc tgttcgtcct gctgcagccg gctgcgtgtc gataccggct
     1621 cgcgcgctac ctggcggaca gcggcatcga cccgcagtgc tgcatcctga cccatcacct
     1681 cccgcatgac cgccgccggc agacgcgctt tcacgtcctc ggcgcgcagt tcgccacgcg
     1741 cgaacaacgc acgcagctgc tcatcgttaa gcccggccag ccgctgcggg tcaggggtgg
     1801 tcttctcgcg gcccagtttg tcggcctgcg tctgcagttc acgctgcagg cgttcttccg
     1861 gactctccgg caatttaatg acaccgtcgg acgcgccgct ctcccgtgca gtggccttga
     1921 gcgtctcgtc gttcacccac accctgccgc cggtcatcgc ctgggggttg aacggcacgt
     1981 cgtcaccctg caggcgtacc accacgccac tgtcgggata gcgtgccgcc aaccggatac
     2041 cgtcctgcat cgtcccggcc agccgggtct cgttgttgcg gctcccctgc agcgccacga
     2101 actgcgcgct gtcactgccg aacactcgcc cctgcgtcgg aagtcccccg acgcttttcc
     2161 cctgggtgtc cgccagcggt gccagccagg cgccggccgg tttaccattg gcgtcataca
     2221 tcggcagcat ggcgtgcggt tgggggtatt tcttgcccgg cgacacatag tgcgccagcg
//
LOCUS       DADTFG010000288         2253 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.290, whole genome shotgun sequence.
ACCESSION   DADTFG010000288 DADTFG010000000
VERSION     DADTFG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.290"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            59..220
                     /locus_tag="J1138_004658"
     CDS             59..220
                     /locus_tag="J1138_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA8456328.1"
                     /translation="MNLLKFGKTYTEIALILGVSERTVRFHINNVLRKLDVTSVRYAI
                     FKATSEGLI"
     gene            complement(248..1138)
                     /locus_tag="J1138_004659"
     CDS             complement(248..1138)
                     /locus_tag="J1138_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA8456329.1"
                     /translation="MNAMRQRFAVVMSDLIRENDNIALVLADITAERFSDVLSDFPDR
                     VINVGIREQLMIDMTGGLALSGMRPFAHTFASFLIERPFEQIKIALNHQNVGAVLVSA
                     GASYDLSVDGRTHQSPADVALLNTLADWNIIIPGNAEEAEYALRMAASDTGLYYIRLS
                     TETNKQTYWSGENGFTVLKSGHQGTVIAVGPVADRVLASTEDLDVTVLYTPVITPFDT
                     ATLRKTLSVPDIAIVEPYLEGTSAHSISKALSDIPHRLLSIGVKRQELRQFGTPEEHI
                     RAHGLDTPGLKERLSVFYRR"
     gene            complement(1188..1907)
                     /locus_tag="J1138_004660"
     CDS             complement(1188..1907)
                     /locus_tag="J1138_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA8456330.1"
                     /translation="MNLTSTPQSKRELLNRYLSLMTGDEKHAPSSISTLDVIQVLYEK
                     VLNYSPGQIQDEYRDRFFLSKGHGPMAFYAVLALKGYISPEQLETYGEYDSKLGYHPD
                     RTKLDCVEISSGSLGHGLPLAIGSIMALRARGITGPRHWVLMGDAELDEGSNFEAIPF
                     AGRRGISELNVVVIDNDSSTLGWPGGISHRFSIEGWEVSSVNGRDHDELYRAFTKEHP
                     HKPHAIIATVESKSGKPRRLL"
     gene            complement(1894..>2253)
                     /locus_tag="J1138_004661"
     CDS             complement(1894..>2253)
                     /locus_tag="J1138_004661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8456331.1"
                     /translation="INYRNNILFFLVVASLLAMGFSGNHLSITVAGALLIDAFIQLIH
                     VNNQRGLFLSCQGNEARAASCYMMSFVTGGAIGGFISSYLYAIFGWNVVLISCAIITM
                     IPLFIKTKTRENFNEPN"
BASE COUNT          659 a          495 c          461 g          638 t
ORIGIN      
        1 tgaacatcat tacaaacaaa aaataaaata tacttttaca atacgggaaa aggaggttat
       61 gaatttactt aaatttggta aaacttacac agagattgca ctgattcttg gtgtaagtga
      121 aagaactgtc agatttcaca ttaataatgt cctcaggaaa ctggatgtga catctgtccg
      181 ctatgcgatt tttaaagcta catccgaagg tttgatctga caaggttttg ccctgtcaga
      241 tcatagatta gcggcgatag aaaactgaaa gacgttcttt caatccgggt gtatccagac
      301 cgtgtgcccg tatgtgctct tccggggtcc cgaattgcct gagttcttgg cgcttaacac
      361 caatggataa taatctgtga ggtatatcgg ataatgcttt agaaatagaa tgtgcagatg
      421 taccttctag gtatggctca acaattgcga tatcaggtac ggaaagtgtt ttccgtaatg
      481 tggcagtgtc gaaaggcgtg atcactggag tgtacagtac agtgacatca agatcctcag
      541 tggatgctaa taccctgtca gcaacaggcc caacagcaat gacagtaccc tgatgacctg
      601 atttcagcac tgtaaaaccg ttttcacctg accagtatgt ttgcttattt gtttctgttg
      661 acaggcgaat gtaatagagg cctgtatcac ttgcagccat acgaagggcg tattcggctt
      721 cttctgcatt tcccggaata atgatattcc agtccgctaa tgtattaagt aaggctacat
      781 ctgctggtga ctgatgtgtc cgaccatcaa ctgacaaatc ataagaagcc ccagcgctga
      841 ccaggacagc accaacattt tgatggttaa gggcgatttt aatctgctca aaggggcgtt
      901 ctataagaaa actggcaaaa gtgtgtgcaa atggacgcat acccgataac gccaaaccgc
      961 ctgtcatgtc aatcatcagt tgttccctga tcccaacatt gatcactcgg tcaggaaaat
     1021 cgcttaagac atcactaaat ctctcggcgg taatatcggc aagaactaaa gcaatattat
     1081 cattttcccg aatcagatct gacatgacta cagcaaaacg ttgcctcatt gcgtttattt
     1141 cggcaaatcg ttgatcccaa gttaaaactt ttttcatttt tattccccta taaaagcctg
     1201 cgaggtttac ctgatttgga ttctacagtt gcaataatgg cgtggggttt atgtggatgc
     1261 tctttggtaa acgcccggta aagttcgtca tgatccctgc cattaacgct gctgacttcc
     1321 caaccctcta tggaaaaccg gtgtgatata ccaccaggcc agccgagagt agacgagtca
     1381 ttatctatga ccacgacatt tagttctgat atcccacgcc tgccagcaaa gggaattgcc
     1441 tcgaaattcg agccttcatc aagttctgcg tctcccataa gtacccagtg tctggggcct
     1501 gtaattcctc tggctctcag ggccataatc gagccaatag ctaaaggtaa accgtgtcct
     1561 agtgatccgc ttgatatttc aacacaatcc agtttggtac ggtccggatg gtaacccagt
     1621 ttgctatcat attcaccgta tgtttccagt tgttccgggg aaatataccc ttttaatgcc
     1681 aatacggcat aaaatgccat aggaccatgc ccctttgaaa ggaagaaacg atcccgatat
     1741 tcatcctgaa tttgtcccgg tgaataattc agcacttttt catacaacac ttggatcaca
     1801 tcaagagtgg agattgatga aggcgcatgt ttttcatctc ctgtcattaa ggacaagtaa
     1861 cgattcagaa gctctctttt actttgaggt gtactagtta ggttcattaa agttttctcg
     1921 tgtttttgtt ttgataaata acggaatcat ggtgatgatt gcacagctaa tcagcactac
     1981 attccatcca aatattgcat agagatagga tgagataaat ccaccaatcg cacctcctgt
     2041 aacaaaactc atcatatagc aactcgccgc tcgtgcttcg ttaccctgac aggaaagaaa
     2101 cagtccacgc tgattattaa catgtattaa ctgaataaat gcatcaatca gaagtgcacc
     2161 cgcgacggta atagataaat ggttgcctga aaatcccatt gccagcagac ttgccacaac
     2221 taaaaagaaa agaatattat ttctataatt tat
//
LOCUS       DADTFG010000289         2239 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.291, whole genome shotgun sequence.
ACCESSION   DADTFG010000289 DADTFG010000000
VERSION     DADTFG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.291"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..324
                     /locus_tag="J1138_004662"
     CDS             <1..324
                     /locus_tag="J1138_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBA8456332.1"
                     /translation="QETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLL
                     EHPELQLMEANYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTV
                     RNSGK"
     gene            321..782
                     /locus_tag="J1138_004663"
     CDS             321..782
                     /locus_tag="J1138_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HBA8456333.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            782..1459
                     /locus_tag="J1138_004664"
     CDS             782..1459
                     /locus_tag="J1138_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBA8456334.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVTAAIACALAPFTPIVTGALFLYLCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTVTTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(1488..1964)
                     /gene="bfr"
                     /locus_tag="J1138_004665"
     CDS             complement(1488..1964)
                     /gene="bfr"
                     /locus_tag="J1138_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBA8456335.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(2036..2230)
                     /gene="bfd"
                     /locus_tag="J1138_004666"
     CDS             complement(2036..2230)
                     /gene="bfd"
                     /locus_tag="J1138_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBA8456336.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
BASE COUNT          574 a          566 c          488 g          611 t
ORIGIN      
        1 caagaaacct ggcaacgtta tttcccgcag atcaaacaca cccacaactt tcgtttttac
       61 tttaagcagc aactcgctca gcaatatcct gaagctgtac cgttgctcta tcatttacaa
      121 acacttctgc ttgaacaccc tgaactgcag ttaatggagg cgaactatag ccagaggcaa
      181 aagtcgctga ccttaaaaat gagtgctaag agtgaagcca atatcgatcg tttctgtgag
      241 ttaacacagt cctggcttcc gatggaaaaa acagaaaaag atccggtcag tggtgtatgg
      301 acagtaagga actcagggaa atgattaaat catggtgggc agaaaaatcc acatcggaaa
      361 aacagatcgt tgcggcactg gcggttctct cgctcggcgt tttttgttgg cttgcggtga
      421 taaaaccgat agatacctac attgaagagc atcagtccca cgcgcaaaaa attaagaagg
      481 acatcaaatg gatgcaggat caagcgagca cccatggttt actcggtcat ccagcgctta
      541 cgcagcccat caaaaatatc cttctcgaag aagcaaaacg cgagaatttg actatcacgc
      601 tggaaaacgg tccggacaat actctgacaa ttaatcctgt cacagctccc ctcgaaaacg
      661 tctcccgctg gctcactacg gcacagataa catacggtat tgttattgaa gaccttcagt
      721 ttactcttgc cggaaacgaa gagatcaccc tcaagcattt gtccttcagg gagcaacaat
      781 aatgacaatg ctactgccgc tcttcattct ggttggtttt attgcaggtt attttgttaa
      841 tgccatggcc tatcacctct cgcctttgga ggataaaacg gcgttaacgt ttcgccaggt
      901 attggttcat ttctggcaaa aaaaatatgc ctggcatgat acagtgcccc tgatactttg
      961 cgttactgct gctatcgcct gcgccctggc acctttcacg cccatcgtga ctggcgcact
     1021 ctttctctat ttatgtttcg cactcacgct cagtgttatt gattttcgca ctcagctcct
     1081 gcccgacaaa ctcaccttac cgctgctctg gcttggcttg gtatttaatg cgcagtctgg
     1141 attaattgat ttacatgatg cggtttacgg cgcggtcgct ggctatgggg tgctgtggtg
     1201 tgtttactgg ggcgtctggt tagtttgtca caaagaggga ttgggctacg gtgatttcaa
     1261 gctactggcc gctgcaggcg cgtggtgtgg ctggcaaacg ttgccaatga tactgctgat
     1321 tgcctcgctg ggtggcattg gttacgccat cgtttcacaa cttctgcaac gccggactgt
     1381 aaccactatc gcattcggcc cgtggcttgc gctcggcagc atgataaacc tgggatatct
     1441 ggcctggatc tcttattaac cgggagggtt cgccctcccg acacggctca accttcttcg
     1501 cgaatctgtg cttgcagata attttgcagt cccatcttct gaatcagatc aagttccgtt
     1561 tccagccagt cgatatggcc ttcttcatcg cgcaaaattt ctatcatcat atcgcggctg
     1621 acgtaatcat gaacgctatc ggcataacca attgcctcac gcaaattctt cgcgccatcc
     1681 agctcaagtg ccagatcaga acgcagcatt tcctcaacat cttcaccaat gttcagtttg
     1741 cccaggtcct gtaagtttgg aagaccttcc agaaaaagaa tgcgctcaat ataacgatcg
     1801 gcgtgtttca tctcatcaat ggattcatga tactccacat cattgagacg tttgagaccc
     1861 cagtttttaa acatccgggc atgaagaaag tactgattga ttgcgacaag ctcatttccc
     1921 aacagtttgt tgagataatt tataacttta gtatcacctt tcattttttg actccttcgc
     1981 ttccactctt tgaagcgtag aacggcttac gagtaagtca aaaaacacac tccgtttatg
     2041 cggactcctt aaactccggc agttgcatta attcatcctc catcacttca cgcgcggccc
     2101 ggacacattt accgcactga ttgccgaccg gaatgaattt ttttaattgc tggaacgagt
     2161 ggggggaaaa ctggcgcaca gcctgacgaa tttttttatc gctgatacca ttacaaagac
     2221 aaacgtacat agagactcc
//
LOCUS       DADTFG010000290         2231 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.292, whole genome shotgun sequence.
ACCESSION   DADTFG010000290 DADTFG010000000
VERSION     DADTFG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.292"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(875..1045)
                     /locus_tag="J1138_004667"
                     /pseudo
     CDS             complement(875..1045)
                     /locus_tag="J1138_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            973..2202
                     /locus_tag="J1138_004668"
     CDS             973..2202
                     /locus_tag="J1138_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HBA8456337.1"
                     /translation="MAPDRCVSRFPASSLFAQRVLPPYDSRQSSASAGGSFTWGAGRG
                     GYGSASRDNIHSNYDSVNEQSGIFAGRGYFDITVGNHTQLDGAVIASTAPADRNSLDT
                     GTLGWSDIHNQADYKASYTGISLSGGSGMSASQMVASNAIAGAANALTGMSGSSGHAE
                     GTTSSAISGGNLIIRDKESQKQDIAGLSRDPENANGSIAPIFDKEKEQKRLQEAQVIS
                     QISGQMSNIVMTYGETEAMKAAWKEHPGMSDAQLRETPEYREVMKGYGTGSSPQMVVQ
                     AITGVLGGLNAGNPGQVLAGGLNPAVAQLIKQSTGDNREANLMAHAVWGALAAQLGGN
                     NAASGAAGAFSGELAARYIIDNYYGGRTDNLSEQERQQISMLATIASGIAGGLAGNST
                     ASAGTGAQAGTRLKTIC"
BASE COUNT          568 a          546 c          588 g          529 t
ORIGIN      
        1 atatttcacg ctatgaaaga taaaactgat acccaccttc agttttgggt caccatcaaa
       61 aaaaaatgtt cgccagatat tccaccactg gcacagttca accctgcttc aaaaattgac
      121 ttattcattg tgctcatttt cgtagtccgt tcaggttgaa taaaactccc ttagtcaaat
      181 gaggaaagac tgagaggtta ttactttagc ttacgttata gctgttttcc tttgcagtat
      241 ttctttgctt ttctgtatac ctttatacct gttataccag atcaaaaaac aagcaatcca
      301 cataacaaaa cgcgttttgt tactgatgtc acaaattgag catatttttg tatctgatag
      361 tttgttacaa acaccacaac caagatgcct atttcgtttc agcatcaact gttaaattat
      421 ctggttgcca acgagaatac tgagcttttt tgtccctaag actgaccgtc aacgcaacaa
      481 tctttgactg tttgacattg tcatgcccgc aggccagcgg atctaaagac atcagactgc
      541 ttgcatcagc cagttgcgta tctggctatg tcccttaatt ggtaagggtt cgaggaactc
      601 aacattaacg catttttcag cgcatcaccg acagtgtata tgaacgattg cactctgacg
      661 atggttgatt tatttggttg cataggggct agctagtctg gtaccggagg ctggatctga
      721 agtactgacc ctaggaataa cgccgggctg actcatattc tgacccattg tcagcccgcg
      781 tgtttactgc tctagctcat ttcctgttgt caggcattca attcccaact gcaggttatg
      841 gcccggtgag cgaacacctg acggcaggta tcactcaccc ggagaaggta aatccctcaa
      901 ggggcagcaa ctctgccagt cgctcctccg gccactccgg cagacgtgtc aggacgtctg
      961 tcagccaggc atgtggctcc agaccgttgc gtttcgcggt ttccagcaag ctcattattt
     1021 gcgcagcgcg ttctcccgcc atacgacagc cgtcagagca gtgcgtcagc gggcggcagt
     1081 tttacctggg gtgctggcag aggtggttat ggcagcgcca gccgcgataa tatccacagc
     1141 aactatgatt ccgttaatga gcagagcggt attttcgcag gccggggtta ttttgatatc
     1201 actgtgggga atcacactca gctcgatggt gcggtgattg cttcgacagc acctgccgac
     1261 aggaactcgc tggacaccgg tactctgggc tggtcggata tccataacca ggcagactat
     1321 aaagccagct ataccggaat cagtttatcg ggtggctcag ggatgtcagc cagccagatg
     1381 gtggcctcaa atgcaatagc cggtgcggcg aacgcactta ccggtatgtc cggcagcagt
     1441 ggacatgctg aaggtaccac gtcatccgcc atcagcggcg gaaatctcat tatccgtgat
     1501 aaggagagtc agaagcagga tattgccggg ctcagccggg acccggaaaa cgccaacggc
     1561 agcattgcac cgatatttga caaggagaaa gaacagaagc gtctgcagga agcgcaggtt
     1621 atcagccaga ttagcggtca gatgagtaat atcgtaatga cgtatggcga aactgaagcg
     1681 atgaaagcag cctggaaaga gcatcctggc atgagcgatg cacagttgcg ggagactccg
     1741 gaatacagag aggtcatgaa aggctacggt acgggcagtt cgccgcagat ggtggtacag
     1801 gccatcacgg gcgtgctggg cggactgaat gcagggaacc cggggcaggt attggcgggc
     1861 ggtctgaatc cggcagtggc acagcttata aaacagtcca ccggcgataa ccgggaagca
     1921 aacctgatgg ctcacgcagt ctggggtgca cttgccgcac aactgggcgg aaacaatgcg
     1981 gcttccggtg ctgctggggc attcagtggt gaacttgcag cccggtatat cattgataac
     2041 tactacggtg gtcgtaccga taacctgagt gaacaggaac gtcagcaaat cagcatgctg
     2101 gccaccattg cctctggtat tgcgggggga cttgcaggca acagtacggc gtctgccggt
     2161 acgggtgcac aggcaggaac tcggctgaaa acaatatgct gaacgtgata gccactgaaa
     2221 cagcagctaa t
//
LOCUS       DADTFG010000291         2207 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.293, whole genome shotgun sequence.
ACCESSION   DADTFG010000291 DADTFG010000000
VERSION     DADTFG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.293"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(77..1003)
                     /locus_tag="J1138_004669"
     CDS             complement(77..1003)
                     /locus_tag="J1138_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA8456338.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            1160..2083
                     /locus_tag="J1138_004670"
     CDS             1160..2083
                     /locus_tag="J1138_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA8456339.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHLQ"
BASE COUNT          655 a          491 c          452 g          609 t
ORIGIN      
        1 tttcaggaga tttattatga ataaacgtat cctcacgatt gcgccattct tcttaccgat
       61 attctgacac acatcattaa ctttctattt ttactctttt ctccaccata aaatcaataa
      121 aagcacgaac tttagagggc attctcaccc gtgatgaaac atatatataa aatccaggaa
      181 atgaaacggc ccaatcctca agaatcggct gtaacttgcc cgcattgata tgttcagcga
      241 ttgcaaaatc cagatgttga atgatacccg ttccattcag cgccattcgt atcatgcttt
      301 cgtcatcatt agaaatatac ttacctgaca cctctatttc gaactcttca ttacccccca
      361 caacattcga aaactcccat gctgagagtg caccgctact tttatgccgc aaccgaagac
      421 aattatggtt actcaattcc tgaggagttt caggaatacc gtgttctttt aaataatctg
      481 gcgatgccac agtaaccagt ttaataagcg gagaaacagg aacggcagtc atatgctcat
      541 ccagtccttg ctcaagacgt atgcccacat cacagccgtc cgcaataatg ttagataaac
      601 cgtcatccat aatgagttct atctttgttt tggggtaacg agtaagaaac tcgccaatat
      661 gaggttcaac aagcatcctt gctgcaacac gggaggaatt tattctcaac agtcctgtag
      721 gttcaagttg ggtatcaccg acatctttca acgcatcatc aatttgcccc aaagccgaca
      781 ccagcacttc atacagatgc tgtccttcct cagtaagcga catattgcgg gtcgtacggt
      841 atatcagctt ggtgttaaga cgttcctcaa gagattttaa attctgggac aatgcggccc
      901 gcgtaatacc catttcagag gctgctctgg aaaaactgcc ggcacgaaca atcagcgcaa
      961 accaggcaag agaaggcaaa attgaaggct ctattttcat cattgtaaac cttaacttaa
     1021 cactgtgata gatattagac cattgatgat aaccaataca agtcatacta ttacgccgtt
     1081 cgcgatatga acacgaatgc cagttgcgga taatagaaat gatatttaaa tctgtttagg
     1141 caaatccctg gagcttgaaa tgaaaactgt ttcgataaaa cacacttact gggatatagc
     1201 tgctgatatc tatttccctg acgattttaa tagtgaaaaa aagtatcctg ccattatcag
     1261 tgcccatcca attggaagct gcaaagaaca gacatcgggt agtgtgtatg gtgccgccct
     1321 ggcaaaagct ggctttatcg tcattgcttt tgatgccagt tttcagggaa gcagcggtgg
     1381 agaaccgcgt tatctggaag atccaacaat gcgagtaaaa gacttcagca ttgtcgttga
     1441 ttaccttacc accttacctt acgttgatgc cgggcgtatt ggtgtactgg gtatttgtgg
     1501 tggaggtgga tatgccatta atgccgcaat gacagaacgt cggattaaag ccattggtac
     1561 tgtcacgggt gcaaactacg ggcgtcttat gcgtgaggga tttacagcgt ttaatcctat
     1621 cggagcactc gaagcaatgg cgcaacaaag aacagacgaa gctaacggcg caaaacttcg
     1681 cgtagatgat ttgctccctt cttctccgga agcagctctt gaagcgggat taacagaaat
     1741 agaccttttt gaagcaacag aatattatcg ttcgccacga ggctgtgcgc ctaatggggt
     1801 aaatcgttcg ctattttcac atcagactgt tgccgtgggc tgggatgctt ttcatcttgc
     1861 agaagtcctg ttaactcaac caataatggt tgtagtgggc gatagagtgg gcgcttttgg
     1921 tgcctatcgt gacggctgcg aaattattgg tcgtgcagcc tcaaaacata aagaactggt
     1981 cgtggttgaa ggttattcac attacgacct gtatgataag ccagaacctg tgaaacaggc
     2041 gctcgataaa ttaattcctt tttacaaaac acatttgcaa taaccttgca gggagattca
     2101 ttatctccct gacatttatc ctttattgac tacacgacaa taatgaaatt actcaaaaag
     2161 aattctatta aagaaaaaag catcaaaaag tagcaactca acttatt
//
LOCUS       DADTFG010000292         2204 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.294, whole genome shotgun sequence.
ACCESSION   DADTFG010000292 DADTFG010000000
VERSION     DADTFG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.294"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>2204
                     /locus_tag="J1138_004671"
     rRNA            <1..>2204
                     /locus_tag="J1138_004671"
                     /product="23S ribosomal RNA"
BASE COUNT          567 a          481 c          699 g          457 t
ORIGIN      
        1 tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg ggagccgaag
       61 ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac ccggtgatct
      121 agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg actaatgttg
      181 aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg gagatagctg
      241 gttctccccg aaagctattt aggtagcgcc tcgtgaattc atctccgggg gtagagcact
      301 gtttcggcaa gggggtcatc ccgacttacc aacccgatgc aaactgcgaa taccggagaa
      361 tgttatcacg ggagacacac ggcgggtgct aacgtccgtc gtgaagaggg aaacaaccca
      421 gaccgccagc taaggtccca aagtcatggt taagtgggaa acgatgtggg aaggcccaga
      481 cagccaggat gttggcttag aagcagccat catttaaaga aagcgtaata gctcactggt
      541 cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat gcaccgaagc tgcggcagcg
      601 acactatgtg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgg cctgtgaggg
      661 ttgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg ggtgaaaagc
      721 ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg tgagtcgacc
      781 cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc tgtacttggt
      841 gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg tcccggttta
      901 agcgtgtagg ctggttttcc aggcaaatcc ggaaaaccaa ggctgaggcg tgatgacgaa
      961 gcactacggt gctgaagcga caaatgccct gcttccagga aaagcctcta agcatcaggt
     1021 aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc aaggcgcttg
     1081 agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag aaggcacgct
     1141 gatatgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata ccagctggct
     1201 gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta tacggtgtga
     1261 cgcctgcccg gtgccggaag gttaattgat ggggttagcg gcaacgcgaa gctcttgatc
     1321 gaagccccgg taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc
     1381 gggtaagttc cgacctgcac gaatggcgta atgatggcca ggctgtctcc acccgagact
     1441 cagtgaaatt gaactcgctg tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg
     1501 tgaaccttta ctatagcttg acactgaaca ttgagccttg atgtgtagga taggtgggag
     1561 gctttgaagt gtggacgcca gtctgcatgg agccgacctt gaaataccac cctttaatgt
     1621 ttgatgttct aacgtgggcc cctaatcggg gttgcggaca gtgtctggtg ggtagtttga
     1681 ctggggcggt ctcctcctaa agagtaacgg aggagcacga aggttggcta atcctggtcg
     1741 gacatcagga ggttagtgca atggcataag ccagcttgac tgcgagcgtg acggcgcgag
     1801 caggtgcgaa agcaggtcat agtgatccgg tggttctgaa tggaagggcc atcgctcaac
     1861 ggataaaagg tactccgggg ataacaggct gataccgccc aagagttcat atcgacggcg
     1921 gtgtttggca cctcgatgtc ggctcatcac atcctggggc tgaagtaggt cccaagggta
     1981 tggctgttcg ccatttaaag tggtacgcga gctgggttta gaacgtcgtg agacagttcg
     2041 gtccctatct gccgtgggcg ctggagaact gaggggggct gctcctagta cgagaggacc
     2101 ggagtggacg catcactggt gttcgggttg tcatgccaat ggcactgccc ggtagctaaa
     2161 tgcggaagag ataagtgctg aaagcatcta agcacgaaac ttgc
//
LOCUS       DADTFG010000293         2165 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.295, whole genome shotgun sequence.
ACCESSION   DADTFG010000293 DADTFG010000000
VERSION     DADTFG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.295"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            235..>2165
                     /locus_tag="J1138_004672"
     CDS             235..>2165
                     /locus_tag="J1138_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456340.1"
                     /translation="MDVPATIEDKDDSTGLVYTVSLNHVTTTDVTVTVTLGSGAGHSD
                     AADYSDIGGAQHNGKIGLHGDTGKVTYDGATTVTIVIPAGSKSVSFIVDPTLEANQDA
                     FNAEGMEKVVATITGTSDNVTAATDIVDNAGASATGVIYDGNAISLRNLDGDFTLKYS
                     LSSSVAEKGDFGYTIGANSGENDPMVTTDYNDTVYVGYYQSGKETTSYSNVANSQDNG
                     PDGTKTDGNQSITTVDLGAGDDLMVIRGNMLANTRVYTGEGNDTFTMDGMNTALRVMY
                     AGSYIFTESGDDIVTIKRTGVTNAGQIYLGSGSDTFIQGDATDNNDTTLSGLLDLGSG
                     TKDISNMPKEYLSVYQDGSNLSLGNDNNIDTATDVNTVTIYGSVSGEILGGYGSDNIT
                     VTKNLTGNISVGDNADTLTAGWIYGGATVSMGDGNDTVTVTDGAYNTTISLGAGDDVF
                     DSTGATLGSAATTIDGGEGNDTIKIGTISNGNITIDAGAGDDIVVLTKDYDTKPVGNQ
                     GSINGGEGSDTLVLAGNISVNLATGKNEGIAGFEKVDMTVGSDLKAGNTAQLVKLTAS
                     DVLGMNDNSTLYISGGANDKVDLGADGAGSLGTFTATATTVKATALDGIEHTYTLYSS
                     VSGANVYIDNNIIDANGVI"
BASE COUNT          633 a          365 c          544 g          623 t
ORIGIN      
        1 ccgacgctga tcccggacag cactggcgat aaagcgacca ttccggcaga taatgtgaag
       61 gataacagtg aagttaccgg ggttgccaaa gatccgagcg gtaatgaaag tgacccgtca
      121 accgtaacct caaaaactga tgtgttacca acagtgagta ttagcgttga gacaacatca
      181 acagatgtca atggtgatgg ttttactggt atagctagtg tcaatgggac cgtaatggat
      241 gttcccgcaa ctattgaaga taaggatgat tccactggac tggtttatac ggtttctctg
      301 aatcatgtca ctacaacaga tgtaacagtt acagttacat tgggtagcgg agctgggcat
      361 tctgatgctg ctgactattc tgatattggt ggagcacagc ataacgggaa aataggcctt
      421 catggtgata ctggaaaagt aacctatgat ggtgcaacaa ccgtaacaat tgttattcct
      481 gccggttcaa aaagtgtttc atttatagtt gatccaacgc ttgaggctaa ccaagatgcc
      541 tttaatgccg agggtatgga aaaagttgtt gcgaccatta cgggaacatc tgataacgtt
      601 acggcagcta ctgacatcgt tgataatgcg ggagcatccg caacaggtgt tatttatgat
      661 ggtaatgcca tttcattacg taatctggac ggtgatttta cactgaaata ttcactatca
      721 tcttctgtcg ccgaaaaagg ggactttggg tataccattg gcgcgaatag cggtgaaaat
      781 gatccaatgg taacaacaga ttacaatgat acggtttatg ttggttatta ccagagtggt
      841 aaagaaacaa catcgtatag taatgttgct aactcacagg ataatgggcc agacggaaca
      901 aaaaccgatg gcaatcaaag cattacgact gttgatttag gtgctggtga tgatctaatg
      961 gtcattcgtg gtaacatgct ggctaataca agagtataca ctggtgaggg taacgatact
     1021 tttactatgg atggtatgaa tactgcatta cgtgttatgt atgcgggtag ttatatcttt
     1081 actgagtctg gcgatgatat tgttacgatc aaacgtactg gtgtaacaaa tgccggacag
     1141 atttatcttg gttcaggtag tgatacattt atccaggggg atgctactga caataacgat
     1201 acaacattat ctggtttatt ggatcttggt agtggtacaa aagatatatc taatatgcct
     1261 aaggaatatc tttctgttta tcaggatggc agtaatttaa gcttgggtaa cgacaacaat
     1321 attgacacag ccacagatgt taataccgtc actatttatg gctctgtatc tggtgaaatt
     1381 ttaggcggat acggtagtga taatattacg gtaactaaaa atctgaccgg taatatttct
     1441 gttggtgata atgccgatac cttaactgct gggtggattt atggtggtgc tactgtttct
     1501 atgggggacg gcaatgatac tgttaccgtc actgatggtg catataacac gactatttca
     1561 ctgggggcgg gagatgatgt atttgattcc actggtgcaa ctttaggttc agctgcgacg
     1621 accattgatg gtggggaggg gaatgacaca atcaaaatag gtactatttc aaatggtaat
     1681 ataaccattg atgcgggtgc cggtgatgat atcgttgttc taacaaaaga ctatgatacc
     1741 aaacctgttg gtaatcaagg ttccattaac ggtggagaag gtagcgatac tctggttctg
     1801 gcaggtaata tttctgtcaa tctggctaca gggaagaatg aaggtattgc cggcttcgag
     1861 aaagtggata tgactgttgg cagcgatctg aaagctggca atacagctca gttagtcaag
     1921 ttaacggcat cggatgtatt gggtatgaat gacaattcaa cgctttatat ttccggtggt
     1981 gccaatgaca aagttgattt gggtgctgac ggtgcgggaa gtctgggtac atttacggca
     2041 acggcaacaa ctgttaaagc aactgcgtta gatggtattg agcatactta tacgctttac
     2101 agcagtgtaa gtggcgcaaa tgtgtatatt gataataata ttattgatgc gaatggtgta
     2161 attta
//
LOCUS       DADTFG010000294         2130 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.296, whole genome shotgun sequence.
ACCESSION   DADTFG010000294 DADTFG010000000
VERSION     DADTFG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.296"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(76..1578)
                     /locus_tag="J1138_004673"
     CDS             complement(76..1578)
                     /locus_tag="J1138_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBA8456341.1"
                     /translation="MAIFHLDFKIVKRSEGMTSVAKAAYHARTRITDDRIGETFDFSH
                     RTDLHGHIILAPVSAPAHIVESSSALWNEVERVERQNNGQTARYFDVAIPVELNNDDK
                     KKLVAEYCQKNFVDKGMIADIAFHDLDSKNPHAHVMLTLKTIGPDGFGKKDRSWNDKK
                     IMIQWRESWATMSNSYLEAAGSEERIDHRSLRTQCADALAQAEEAFSAEEKAFWLAKA
                     TETNRPAMQRVHRAKWNDTESQEQRAAEQAQRDQQIEEAKKVYTTFRELPLEIVVDVR
                     SFTITHLAEPEEIIIPDYPATVEPQPAMATPTTSRRPAAKSYRNPNKVSKVNVSGKKS
                     PVLVVPEPKASTKLKTPSLQNTRAVNRAPLHIRKQTKPRQNGLFKRFTLLIVDFLKER
                     FVWARRKTDTTDADHDKRIAENYVFDEVLGVNVPRSEFERRAKFNNNQTSQEAGVYGN
                     ESNINKTVRFPNRFEQEQTEVNKHMEFILSIGGKPSMPKLKQTNRKSNIK"
BASE COUNT          564 a          417 c          472 g          677 t
ORIGIN      
        1 tgttttcata tgctatcaca ttttgagcct atgtaacatg catttgatta aaaattaata
       61 gtggggataa cattactatt tgatgttaga ttttctgtta gtttgtttta actttggcat
      121 agaaggtttt ccaccaatag acagtataaa ttccatgtgt ttattgacct ccgtttgttc
      181 ttgctcaaaa cgatttggga aacgaacagt cttgttaata tttgattcgt taccatagac
      241 tcctgcttct tgtgatgttt ggttattgtt aaacttagcg cgtctttcaa actccgagcg
      301 tggaacattg acacccagca cttcatcaaa cacataattt tcagcgatac gcttatcatg
      361 gtcagcatca gtggtatcag ttttccttct cgcccataca aatctctctt tcaggaagtc
      421 tacgatgagt agtgtgaaac gtttaaacaa accattttgt cgtggcttgg tctgctttct
      481 gatatgtagg ggagcacggt tcaccgctct ggtgttttga agagatgggg ttttcaattt
      541 agtggatgct tttggttcag gaacaaccag aacaggggat ttcttccctg aaacatttac
      601 tttactgacc ttattgggat tacggtaaga ttttgctgct ggtcttctgc ttgtggtagg
      661 tgtcgccata gccggttgcg gttctactgt tgctgggtag tcagggataa tgatctcttc
      721 gggttcagca agatgtgtaa tcgtaaagct tctgacatca accacgatct ccagtggtag
      781 ctcacggaat gtcgtataga ccttcttcgc ttcctctatt tgctgatcac gttgagcttg
      841 ttcggcagct cgttgttcct gtgactcggt gtcattccat ttagcacgat gaacacgttg
      901 cattgccggg cggtttgttt ctgttgcttt ggcaagccag aatgctttct cttcagcgct
      961 gaatgcttcc tctgcttgag caagggcatc agcacattgt gtcctgagag aacggtgatc
     1021 gatacgttct tcactgcctg cggcttcaag gtagctgttt gacatggtgg cccatgattc
     1081 acgccattgg atcattatct tcttatcatt ccagcttctg tcttttttac cgaagccgtc
     1141 tggaccaatg gttttcaggg ttaacatgac gtgagcatgg ggattcttac tatccaagtc
     1201 atgaaatgcg atatcagcaa tcattccttt atcaacaaag ttcttctgac agtattcggc
     1261 aaccaatttt ttcttatcat cgttgttgag ttctacaggt atagcaacat caaaataacg
     1321 ggctgtttga ccgttgttct ggcgttccac tctttcaact tcattccaga gtgctgacga
     1381 gctttcaaca atgtgagcag gtgcggaaac aggcgctaat ataatatgac catgaagatc
     1441 tgttctgtgg ctgaaatcaa atgtttctcc aatgcgatca tctgtaatac gtgtacgggc
     1501 atgataggcc gctttcgcaa cggaagtcat gccttcgctt cgtttcacaa ttttaaaatc
     1561 cagatgaaaa atcgccatta aaaatataac ctcaaatata tgtgctgctg ttagtttttc
     1621 gccagaaaaa cttcggggtt gacgttgacc ttggttttgc ttttagcctg cggcaggcaa
     1681 ccgtcggtag accccacaca ctgcgtagca gtgtataggt gggcattgtc tttaaaaaaa
     1741 taagccaatg taaaatgccc ctcttattaa ttaatacgaa atatatgtat ggttgtaaaa
     1801 taatacgccg ggttaagcgg cgcaaactgg attaaaggtg taggtttcac gtccacagga
     1861 caaaaagagt aaagataagg cccgtaaggg caaaagaagt attaattatt cccgccgtaa
     1921 ggcggggctg ctaaatttga tgaaaaaaac gaataaaaca gacagtgtta tgtgttgcac
     1981 aattcacagg ttgagattgg gaaatagagt ttgaaaatga caggtaaaaa taaaagcacg
     2041 aatgcgcaaa aataagtaac gcggagagtg cggggtattt taagttagta attactatca
     2101 tatgcgttgc acatatttat aaagaaatgt
//
LOCUS       DADTFG010000295         2123 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.297, whole genome shotgun sequence.
ACCESSION   DADTFG010000295 DADTFG010000000
VERSION     DADTFG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.297"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..1715)
                     /locus_tag="J1138_004674"
     CDS             complement(<1..1715)
                     /locus_tag="J1138_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBA8456342.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKGAGVAVALSLAA
                     VTSVPALAADSIVQAGETVNGGTLENHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTVNQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATV"
BASE COUNT          416 a          613 c          559 g          535 t
ORIGIN      
        1 accgtggcgt tatcggggat attccaggtg gcaccggagg cgagagtgac attcgtgggg
       61 tcaatggcac cgttcagcac agtgctgccg gtcagcttca gggctgtacc gcgctgagca
      121 atgacatccg tggtgacggt actgtcgttc agcgtcagcg tgccttcatt caggttgacg
      181 gctttctggg tgagtgtggt gttgctgaca gtgagtgtgc cacttcctga tttttccacg
      241 ctgccgttac cggtgagaga gcctccctgc aggagtgcat cgccttcacg gatggtcagg
      301 gtatcgttgt tcaccgtctt cccggaaagg gtaagtatgg cgccgttatt cagcgtggtg
      361 gtgcccgcca gtgtgccgcc cctggcggtg aacagtccgc catttaccgt ggtatccgtg
      421 gccgtatcac cggcgttcag cgtgaatgaa ctgccttttt ccagcatcag ggcatccgcc
      481 tgaccgcctc cgatactgaa tgcctttccg tcgctccggg taccactgac agcggcaccg
      541 gaatcggcca gcagtacacc gccatttccc atggatacgg tggtggcggt accaccgttg
      601 cggacatcca gcgttccgcc atcatccacg cgggtattag tggctgtgtg tccggtcagc
      661 acatccaggc gtccgccatt ttccagtacg acattatcag ctttaccctc cacaaccgag
      721 aatgctccaa ggcggtttat gccggtaacg gttgcagccg tactggtaac cagtgcaccg
      781 cccgtgttct gggtgactgc agtggcttcc ccgccggcat gtaccctcag ttcaccgttc
      841 tgattaacgg tggtgttacc gacagcacca cctgccttaa caacctgcca gcccccctcg
      901 ttaattaccg tgttcagcgc aagtccgtcc ttgtgcacat actggtaacc gccattcagt
      961 gtggtattca gggcccttcc gtgtaccgac tggtcaccac ctgcgtaaat gagagtgtca
     1021 cgggctatcc cggaaaataa gataatctgc cgtccatttt tgttgatggt ggtggattct
     1081 gctgttccct ggaccttctg acccgtgtac gagttgtcag aatccgggcc gccttctgcg
     1141 ccggtgttga tgatggttcc tgttgccagc ccgccacttt taacgctctg gtaaccgtcg
     1201 cggttgataa ttgtaccggt ggcaaccccg ccctcatgaa cccactgctc gcctctgtta
     1261 ttcagcgtgg tgttcaccgc cagcccgttc aggctctgtc ccccgccgtc acgaataacc
     1321 gtatcactgg ctgttccccc ctccagcacg acctgacgac catttgtggt aacagtggtg
     1381 tttccggcta tcccgccatt ctgtatccat tgcccaccgg tgttttcttc actgtccggc
     1441 cccagttcca gcccggtact gatggtcatt ccgttggccg taccgaggac aatctggttg
     1501 tcatgatttt ccagtgttcc gccgttcacg gtttcacctg cctgtacaat actgtcagca
     1561 gccagggccg ggactgacgt gacagcagca agagacagag caaccgccac accggcgcct
     1621 tttccccgtg agcgggccag ttcggaggcc accaccaggg tgcccgtaat gtgattccat
     1681 accagcctgt agctggtgtt cagatgtcgt ttcatcagct tttccttaca gagggtgaat
     1741 aaaaaagccg gtacccacaa atgtggatac cggcaggtac aaaacagcat ggtcagataa
     1801 agtggttttt cccggacata aatgcttccc ctctctcatc cgccagagaa gtatcagtcc
     1861 gttgtgtgca attccgttct gcatgatgaa gagattcagc tgccttttcg caggccatta
     1921 accactataa acgatcgatg gtaacgatcg atacctgttt tatcgatcgt tttattctat
     1981 taataacaag gcagccacgc aagagaaaca ggaggagtat gaggggtttg tgtcatgttt
     2041 tcagttggtt aacgtaatgt aatcaggcat ttccctggca gagtgttcag aagaaaatcc
     2101 agttccatac agcgcgggca aca
//
LOCUS       DADTFG010000296         2119 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.298, whole genome shotgun sequence.
ACCESSION   DADTFG010000296 DADTFG010000000
VERSION     DADTFG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.298"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..218)
                     /locus_tag="J1138_004675"
     CDS             complement(<1..218)
                     /locus_tag="J1138_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA8456343.1"
                     /translation="MFKEKLQDYTEDEFLNFLGGLRSSMKDGKSLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPE"
     gene            complement(221..2002)
                     /gene="hcp"
                     /locus_tag="J1138_004676"
     CDS             complement(221..2002)
                     /gene="hcp"
                     /locus_tag="J1138_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HBA8456344.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECVNVNYDYILCKHLIANTEFDWLAFSAGYNSLFI
                     PPKNPPASNLNPEPLPVVNLPLSPPAVKPVYAKSCLKEKGCTDAGTAEEPAENFGQVA
                     IFALPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVEGPLITWTPENPEHGYVPG
                     HTGNDRPPLEQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGRGVLLSPGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSQDPELIAQFSSGNQTRIKQGLTAKAPIDGWYYGPKEIVKKFQIHHRVAV
                     EYGGSVYDIDNLRIVTPRLHDEIHYRR"
BASE COUNT          552 a          519 c          454 g          594 t
ORIGIN      
        1 ttttcaggct tatcatctcc ttgactttta gggtagaaaa ttagatcgct ccctgacgga
       61 tgctgtgtaa tttcaataaa atgatctact aatgaatccc agtacatttc tagctcctta
      121 ccttttaagg attttccatc tttcatagat gatctcaatc cgcctaaaaa attaagaaat
      181 tcatcctcgg tataatcctg gagcttttct ttaaacatat ttatctcctg tagtgaatct
      241 catcgtgtag tctaggggta acaattcgta aattatcaat atcgtatacg ctgccgccat
      301 attcaactgc aacacgatga tgtatctgga attttttaac tatttcttta gggccataat
      361 accaaccgtc aataggtgct tttgcggtta atccttgttt tatacgtgtt tggttaccac
      421 ttgaaaactg cgctatcaac tccggatcct gactcacctc cagccacagc gcctcgcgaa
      481 actcatcaaa ggttttaaac tcccgtccgc gcaatttatc agcaatatgc gccgggattg
      541 gtgcgccgtt gccgttattg gataccgaca tatccagcca gcgagttcct ggtgaaagca
      601 ggacgccgcg ccctgttaca acaccaggca gctttcgcgg atcctccttc aggtaaacat
      661 agatgggctt aataccggat cccgccggaa aaaccagaat ataatcgttg aattctttcg
      721 gatcaggaac cgggaatggc ggcgtggtat aggtatcggt gccgtccgga atcggcgtca
      781 ccagaatggt gggctgctcc agcggcggcc tgtcgttacc ggtatggccc ggaacatacc
      841 catgttccgg attttccggc gtccaggtaa tcagggggcc ctcgacatcg tgagcggggg
      901 taaattcata acgctgcgtc tgctcgttcc actgcatgtt cgccacgcgg acgttttcat
      961 acggcgtacc ttcacccgta tgaacaccat acacctgagg cttgccgtga atgtcagttc
     1021 cccagaaaaa acgtacccgg gtggtggcgg tactgcctgc tagtgctttc tgccgcagtt
     1081 cttcctcata ccagaactca tcacgtccgg gcacgcgagc actgccctcg cccgcagagg
     1141 gaatatacaa agcagatgcc aacgcaccga tgtaagagcc gcgggttgca ctgagtatgc
     1201 cactgagcga ccattttccc catatttgag ccgccaacac actacccgga aacagtagca
     1261 tggtttgagc ttctgcgggt tgcccaacat cgttagcctc cggatggtgg tatccacagc
     1321 agtcatcaac cactggcaga gcaaaaatcg ctacttgccc gaagttttca gcgggttctt
     1381 ctgccgttcc ggcatctgta catcccttct ccttcagaca ggatttggca tagaccggtt
     1441 taaccgctgg tggagagagt ggaaggttaa caactggtag cggctcaggg ttaagattac
     1501 tggcaggtgg gtttttaggt ggaataaata agctattata gccagcagaa aaggccaacc
     1561 agtcaaattc cgtattggct attagatgtt tgcataatat gtagtcataa ttaacattta
     1621 cacattcggt aggaagatta ttgtcagaaa tgtttacatg aatagcagtt aatgaagcat
     1681 ttcttagctg aatataataa taccgttccc agcgcccgct tttatttata cgatacaaat
     1741 cgatttcaat aaataaccgc tcattctgat tgatggcatt agacagtagc ggtgagcttt
     1801 tatcaatgag tttgcaaaat tgtagcccct gtagattaac gcctttacca gtactggtaa
     1861 tggcgttggt gagtgcaaag gcaaaaattt catcttcatg gccaacctgc cagcgattac
     1921 caaccgacgc atacgtccca cagcctgaag aaatatcgcc ctgcttctca ccgattattc
     1981 tcaggtaaac aatatcgccc actttatttc attcccgttt atccttaact tttttgcatt
     2041 aaatagaaaa cttatttctc gatcaataag aaacgctttt aatgaaaaat tatttcaatt
     2101 acttaacgtt ttcacttag
//
LOCUS       DADTFG010000297         2109 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.299, whole genome shotgun sequence.
ACCESSION   DADTFG010000297 DADTFG010000000
VERSION     DADTFG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.299"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(1..273)
                     /locus_tag="J1138_004677"
     CDS             complement(1..273)
                     /locus_tag="J1138_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HBA8456345.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFTGKKINRIH"
     gene            complement(316..552)
                     /locus_tag="J1138_004678"
     CDS             complement(316..552)
                     /locus_tag="J1138_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HBA8456346.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            866..2077
                     /gene="bluF"
                     /locus_tag="J1138_004679"
     CDS             866..2077
                     /gene="bluF"
                     /locus_tag="J1138_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBA8456347.1"
                     /translation="MLTTLIYRSHIRGDEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDEEIYTAD
                     LKSKALAFAMVHALKLGDKMISINLLPMTLVNKPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
BASE COUNT          605 a          436 c          413 g          655 t
ORIGIN      
        1 ttaatgtatt ctgtttattt tcttaccagt gaacatactt ttattgtcag ctatcgctgt
       61 tcttcggttt ggccctcctg tatcactgtt gctaaaatca cgaattatgc tgtcaattaa
      121 atctcgtcgg aaagtatttt cctcagccga tttcattacg agtaattgct taataagagt
      181 ctgcaacgaa aaatttatct tcatgcgcag tatctggagc accgcctggc cagtaatttc
      241 atattttata ctttcattat cagatgtctt cataacgact tcatagctaa cctgttaaat
      301 aatcaggctg ttgcattatt caaaaagcaa cccaattagt gcattataat gtttctgttc
      361 ctcttcccct gtcgcttgtt ccagccggca aagaagtttg gcacaaagcg actttcgact
      421 cagattacgt ccatctttca aaatctcagt cacaatttcc ccgagagttt cctgctgagt
      481 aggcaagtta gcttttgcaa agtaacgagt gattgctcct gcagaatctg aagtcactga
      541 attttgatgc atgctacgcc tctgttaaaa atgttaatca atgttaagtg aggtaacaat
      601 agcaaacttg tacgaaatat gtgtacagaa tgataaatta atttttcgca attagtgcta
      661 attcatataa aacaatgcat tacgataaaa catattgcat catttaaatg acaacctata
      721 caagtttttt cgccaaaccg tcttcaacct tttccacttt ttatcaatgt aaagaaacgg
      781 ttatgttttg gtattaaaag aaagacacaa gaagatatgt ctgttaccgt cttactctcg
      841 cctcgcccat taccctggat tgttaatgct taccaccctt atttatcgta gccatatacg
      901 tggcgacgaa cctgtcaaaa aaattgaaga aatggtttcg atagcaaatc gcaggaacat
      961 gcggtctgac gtaacaggga tcttactgtt taatggttct cattttttcc agcttctgga
     1021 aggtccggaa gaacaggttc aaatgatata tcgggccata tgccaggatc cacggcacta
     1081 taatattgtt gaactgttgt gcgattacgc gcctgctcgc cgttttggca aagcaggaat
     1141 ggaattattt gatttacgcc tgcacgagcg agatgacgtt ttacaggccg tattcgacaa
     1201 aggcacatca aaatttcagc taacttatga tgacagagcg ctacaatttt ttcgtacttt
     1261 tgtccttgca actgaacaat caacctattt cgagattcct gctgaagact cctggctttt
     1321 tatcgctgac ggatctgata aagaacttga ttcttgtacc ctttcatcaa atataaacga
     1381 ccactttgcc tttcatccta ttgtcgaccc tttatcgcgg cggataatcg cttttgaagc
     1441 cattgtgcaa aaaaatgaag acagcccgtc agctatagcg gttgggcagc gtaaagacga
     1501 ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca tttgcgatgg tacacgcact
     1561 taagctcggt gataaaatga tttcaatcaa tctattacct atgaccctgg ttaacaaacc
     1621 tgacgcagtc tcttttttac ttgatgaaat aaaggccaat gctctggtac ctgaacaaat
     1681 catcgttgaa tttactgaaa gtgaagtcat atctcggttt gatgagtttg ccgaagcggt
     1741 taaatcgcta aaggcggccg gtatcagtgt agcaattgat cattttggcg caggttttgc
     1801 tggtttgtta ctcctgtcac gcttccagcc tgacagaatt aaaatcagtc aggaattgat
     1861 taccaatgtt cataaaagcg gaccacggca ggcaattatt caggcgatca tgaaatgctg
     1921 tacatcactt gaaattcaag tctctgctat gggcgtggca acaccagaag agtggatgtg
     1981 gcttgaatct gcaggaattg agatgtttca gggagatctg ttttccaaag ctaaattgaa
     2041 tggtatccct tcagttgcgt ggccggagaa aaaataattt tcagcacatt atttcgcatg
     2101 atttctgga
//
LOCUS       DADTFG010000298         2095 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.300, whole genome shotgun sequence.
ACCESSION   DADTFG010000298 DADTFG010000000
VERSION     DADTFG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.300"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..239
                     /locus_tag="J1138_004680"
     CDS             <1..239
                     /locus_tag="J1138_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8456348.1"
                     /translation="SGLQRASVNDEHFSLLIEISPIRSEKTIVALREYLVDGYSKQES
                     CNRNNVSNSYFCLCLKKLNYTHGIVSVLKYFYQT"
     gene            1583..1717
                     /locus_tag="J1138_004681"
                     /pseudo
     CDS             1583..1717
                     /locus_tag="J1138_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          619 a          432 c          421 g          623 t
ORIGIN      
        1 attcaggatt acaacgagca agtgtaaatg atgagcactt ttcgcttctg attgaaatat
       61 caccaatcag aagtgaaaaa acaattgtgg ctcttagaga atatttggtt gatggttact
      121 caaaacaaga aagctgtaac agaaataatg tatccaatag ttatttttgt ttatgtctga
      181 aaaaactgaa ttatacccat ggtattgtat ctgtcctgaa atatttctat caaacataac
      241 atacaccata atatcacttt acctttttct aaatacagct gagctttatg gcttggctga
      301 taaagtgata cgacgatgaa aactaaattt gtttaacaat cccgggcgtc ggtcctccga
      361 ttgctgcagt atttctaagc aaagttgatg ccacatagtt ctctggcctg tgataatcat
      421 cgacatgata cggattagct cctccgctag cacagctctg gcggaaaaca gcgccttcct
      481 tctgtgacaa aaaatagcaa ctagtgtctg ataactttca ttatccgtga tgcgaactct
      541 gtaatgcaca gtgtgaaaaa tgcgtatttt catggaagga gagcgctcaa taacggcgtt
      601 tttcaagtta attgtggact tttgtaatat aattatgtcg cttctttttc tggattaagc
      661 tacacatagc tgaatgtgtt cccaaccacg aaactgtttg cttcagctct caggatgatg
      721 cttgagacct tctctataaa ctgtttttct gacttccagt atttgtatat caattccgtg
      781 atcgtgtcca tccggagtaa catacttaat accgctatcc ttgtgttcct ggttgtgaac
      841 ctgcttaact aacctctggt ttcatctata attcgagaac cattctctgg ccaccatgga
      901 taatatttaa tattccagaa aaaatcggaa taaggcttat cattgctaac ctagggacga
      961 gaataagaac ttaatacgcc aagtatgtac attttgccag taacgtacag ctacgcattg
     1021 ctcctccatt atcagaatgc agcacgaaat ttcggaatgg agcatttttc attccatgtg
     1081 gcacgttgaa gcagtcctgc cgtatactac ccagattctt tcgtaaatac atctgcaaca
     1141 ataatttttc aactatagat atcgacaatc atatagggat gaaaatattg tgccactggt
     1201 actgcagtaa tcatgtctgc tcacgcataa tttttgctga tccgctggtt cacatcagcg
     1261 ggggtacaaa atttatagca ttaactggga ttaagagctg aaggtgtggc tggaaaacgg
     1321 gcagcaacgg tagcaggtct tcgttgctgt gcagatactc ttcttcgcag ggttatcaac
     1381 accctggagg cgaaccgtca agttcgtctc atgtcggtat cgattatttg gcgtggcgtt
     1441 gggagcaccg gtacggtatg ttaatagtca atctcgatac tcaacgcttc tagtcctgct
     1501 tccctgtcgc gattaacgta tgctagtggc ttggttcaga aattatcagg aaatacagat
     1561 tgtctcgcgt gatcgctatt gattccgtga tgcgcttaaa aagcaacttg ctcagctagg
     1621 cacataccac aaccaggtct ccgaagaaca cctgccatta ctggcagcag aagaactaac
     1681 ctgcagtgaa aacaggaagt cagagcgtct gctcaaacag gttatccgtc aggaagagga
     1741 aaaaatgttc tgcctattct ctgctaaatt tattccacag gttcctagtg gaacgccgtt
     1801 tacatgaaaa ggttgcttga cgctctgaag cgtaatgtct tgaaaattaa tgttcaacta
     1861 ttatttcatt tatcactgtt aggttgggaa tgaatcaaac tgacagggaa ttatatggaa
     1921 aatcaaccca ataccggctt cggataagta tcggttttta ataccagcgg aaataggtaa
     1981 ggtcaaaaaa cagccttaac gtacaatgat ttttatatcc aaacagcccc tcacccacta
     2041 ccgccttggg cggcacatga gggatggtag ataatatcta taatatagat ataac
//
LOCUS       DADTFG010000299         2074 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.301, whole genome shotgun sequence.
ACCESSION   DADTFG010000299 DADTFG010000000
VERSION     DADTFG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.301"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(32..1315)
                     /locus_tag="J1138_004682"
     CDS             complement(32..1315)
                     /locus_tag="J1138_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA8456349.1"
                     /translation="MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIV
                     GAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGL
                     GAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
                     WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLA
                     TGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKI
                     GRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMA
                     EMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLIS
                     ALKMPEVKDRDLSALEDAAEDQLRVVRAAQPSRSL"
     gene            1919..2032
                     /locus_tag="J1138_004683"
     CDS             1919..2032
                     /locus_tag="J1138_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456350.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
BASE COUNT          623 a          509 c          391 g          551 t
ORIGIN      
        1 cccctctccg gtaagagaag ggattgagga tttaaagact tctggaaggt tgcgcagctc
       61 tgacaacacg cagttgatct tccgcagcgt cttccagcgc acttaaatca cggtctttca
      121 cttctggcat tttcagcgca gagattaaac caatcactga atatgccatg atcataatgg
      181 cgatcggata ccaggattcc gtcatggtgc agaaaatacc cgccaggata ggaccaaaac
      241 cggaagcgat aagaccacca atttctttag aaatagccat ccgggtaaag cggtttttac
      301 agccgaacat ttctgccatg gtaatgtttt ccagagcaaa taatcccagc accgcacagt
      361 tatgaatcac aatcagtgca accataatgg tgctcggggc atagctttta tctacgatga
      421 tagaaagcat tggccacgcc agcacaatcg cagaggtatt cataataata tacgggatcc
      481 ggcgacctat tttatcggat aaccaaccaa ggaacggaat ggtcataaag ccgagaatcg
      541 aactgatcat caatgcatct gttggaattg ctttgttaaa caataacgtc tgcactaaat
      601 agcctgcaag gaaagtctga attaaacctg agttaccagc ctgaccaaaa cgcagccctg
      661 ttgccagcca gaaggatttg ctctggaaca tgctaccagc aggtgcaggt tttgctgtcg
      721 gttggttgct gtcgttaacc ttctcaaaga ccgggctttc tttcagattc atacgcaacc
      781 agatagcaaa gaccatcacg acaacgctcg ccaggaaagg tatacgccat ccccacgcca
      841 gcagttcctc tttactgaga atgaagaaca taaaggccca gattgccgtt gcgctcaagg
      901 ttccgcagtt agttcccata gccacaaatg aggagataat tccgcgctta ccttttggcg
      961 catattccgc cagcatcgta ccggcaccgg aaatttccgc acctgcaccc aacccctgaa
     1021 taatacgcag cgtcaccagc aagatggggg caaaaacacc aatttgtgca taagtcggta
     1081 acacaccaat taaggtggta cagatcccca tcatggtgat ggtaataaag agcacttttt
     1141 tacgccctat tctgtcgccc attttgccga aaataaatgc tccgacaata cgtgccacat
     1201 aacctgcacc ataggttccc attgccagaa ttaacgccat tgccgttgat gattcaggaa
     1261 aaaatatttc atgaaacact aacgctgcgc cgagcgaata taactggaag tccataattc
     1321 acaggtgttt tttcccatcc tgtggtttcc ttggcgtttt ctaggttttt tcagatagtt
     1381 gcatttttta aaaagcatcc taagtttgaa ctcagtgtct atctggggcc tatttctgtc
     1441 ccatatatgc cccaaaaaaa ctccccaaca gataagtagt tttttcatgg atttacgcgt
     1501 aaaatcaata acggctggaa atcattcaat actcgcacta tcgaaaaatt taccagccaa
     1561 ccgcagcacg ttcttgcata aggcgtgtct gcggtttttc aactattcag atacatcact
     1621 cccatcacat tcattcctcc gcatcaaagg catataggct atatcacctt gatatttttc
     1681 ttcttcagat aaaaactgtt atctatgtat acttttaaac ccaatccgtg tagagtctct
     1741 gcataagata gtttgcagtt gccacttcag cttgcgccat aaaccgcctg atttttgccg
     1801 ccatctgtta gcattcctgt atacctgaaa cgacaatgtt tatctacgaa ctttaagaac
     1861 acccaggata aaaattgtca actatatcat atataacaca ttactaattc gaggctatat
     1921 gaacagcata ctgataatca cttcgctcct tatcatattc agcattttta gtcatgctct
     1981 aataaaatta gggattggca tatccaataa cccagacaaa accgatgtat aagtcaacat
     2041 atcctgaatc agacatacaa tatcgcaatg aaaa
//
LOCUS       DADTFG010000300         2063 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.302, whole genome shotgun sequence.
ACCESSION   DADTFG010000300 DADTFG010000000
VERSION     DADTFG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.302"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..422
                     /gene="psiB"
                     /locus_tag="J1138_004684"
     CDS             <1..422
                     /gene="psiB"
                     /locus_tag="J1138_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBA8456351.1"
                     /translation="EKLESMSADELEQYRDRGREYRVMLNYAVLSQLAVPEGWRVVAE
                     EGCEFCGRVPVVCRISPAGDEATALYLCSAGAEVPNWSMTLPFNGGQSLAWLYLDEHY
                     TPATVNRVLHTVAGYYRLGIRRPEKLAVALRMGGHCL"
     gene            419..1144
                     /locus_tag="J1138_004685"
     CDS             419..1144
                     /locus_tag="J1138_004685"
                     /inference="COORDINATES: protein motif:HMM:NF018636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBA8456352.1"
                     /translation="MIPNHLALVPWHPYRQAVWQAIAQVEARREAGRRLPVYPYATAF
                     FRQLTGRPTLSAKDLRMIDVTYRPGDRRRATRKEDYMDALDTLIASRGEHCYSPLPGD
                     TRDTLFPEVNRRRRQRFEHRLTMKHTRQARIDDNIRQHKRRRYQVRKAQAEIELTFIT
                     PGELNRWVRRAKQQGIADCDLFGLVQAWTSRFPCLAELDCYLWSARPFWENCLQVSLI
                     NGDLSDADRADNDARIPNRLMCC"
     gene            1335..1586
                     /locus_tag="J1138_004686"
     CDS             1335..1586
                     /locus_tag="J1138_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBA8456353.1"
                     /translation="MPNIILSDTSASISELKKNPMETVGAGAGFPVAILNRNKPEFYC
                     VPAALYEQMLDALDDQELVRLVNERRSEALVDIDLDQFA"
     gene            1583..1870
                     /locus_tag="J1138_004687"
     CDS             1583..1870
                     /locus_tag="J1138_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA8456354.1"
                     /translation="MTYTVKFRESALKEWGKLDKSLQQQFAKKLKKCCENPHIPSAKL
                     RGMPGCYKIKLRASGFRLVYEVIDDCLIIAVVAVGKRERGDVYQLASERLK"
BASE COUNT          457 a          503 c          639 g          464 t
ORIGIN      
        1 tggaaaaact tgagtcgatg agcgccgacg aactggaaca gtaccgagat cggggaaggg
       61 agtaccgggt aatgctcaac tacgcggtgc tgagtcaact ggcggtgccg gaaggctggc
      121 gcgtggttgc ggaagagggc tgcgagttct gcgggcgcgt accggtggta tgccggataa
      181 gcccggcagg ggatgaggcg acagcgctct acttgtgcag tgcgggcgcg gaggtgccga
      241 actggtcaat gacgctgccg tttaacggcg gtcagtcgct ggcgtggctg tacctggatg
      301 agcattacac cccggccacg gtcaaccggg tgctgcatac cgtggccggc tattaccggc
      361 tggggatccg gcggccggaa aagctggcag tcgcgttgcg gatgggaggg cattgcctgt
      421 gatcccaaat catctcgctc tggtgccgtg gcacccgtat cggcaggccg tctggcaggc
      481 catcgcgcag gtcgaagccc ggcgtgaagc gggcagacgg ttgcccgtct acccatatgc
      541 tacggcgttt ttccgccagt taacaggacg gcccacactt tcagccaagg atcttcgcat
      601 gattgatgta acctaccggc ccggcgaccg ccgccgtgcc acgcgcaaag aagactatat
      661 ggacgcgctc gacaccctta tcgccagcag gggcgagcac tgttattctc cgttgccggg
      721 cgacacccgc gacacgttgt ttccggaagt gaaccgccgc cgccgtcagc ggttcgagca
      781 tcggctgacg atgaaacaca cccggcaggc gcgcatcgat gacaacatcc gacagcataa
      841 acggcggcgt taccaggtgc ggaaagcaca ggcggagata gagcttacgt tcatcactcc
      901 gggggaactt aatcgctggg tacgccgtgc taagcagcag ggaatagcgg attgtgactt
      961 gttcgggttg gtacaggcgt ggacgagtcg tttcccatgc cttgccgaac tagattgcta
     1021 cctctggtca gcgagaccat tctgggaaaa ttgtctacag gtcagtctga tcaacggcga
     1081 tctctccgat gctgacaggg ctgataatga tgcccgaatt cccaatcgtc tgatgtgctg
     1141 ctagcgcagc aatgtttcgg ggagatcggt cagcgcgcct gggagcatgc acgttacaga
     1201 ctggcgttcg agttggcggt ggagtattcg tacaactgat cagtaagacg atattttatc
     1261 aaaaagacct gttaacaggt ctttttggtg gtataatatg acctgttaag tggtcttatt
     1321 gagtcgagga taacatgcca aatatcattt tgagtgacac aagcgcgagc atcagcgagc
     1381 tgaagaaaaa ccctatggaa accgttggcg ccggtgccgg ttttcctgtg gccatactga
     1441 accgcaataa gccagaattt tattgtgtgc cggcggcgct ctacgagcaa atgctcgatg
     1501 ccctcgatga tcaggagctg gtgagattgg tcaatgagcg ccgtagcgaa gcgctggttg
     1561 atatcgatct ggatcagttc gcatgacgta tacggtcaaa tttcgtgaat ccgcgctgaa
     1621 agagtggggc aagctggaca agtcgctaca gcagcagttc gctaaaaagc tgaaaaaatg
     1681 ctgcgagaac ccgcatatac cctcggcaaa actacggggg atgccggggt gttacaaaat
     1741 caagctgcgc gcctcgggtt ttcgtctggt ttatgaggtg atcgacgatt gcctgattat
     1801 cgcggtggtt gccgttggca aacgtgagcg cggtgatgtg taccagttgg ccagcgaacg
     1861 tcttaagtga acagatgtgc ctaagcacaa gagctattca aatgaggtga ggttaataac
     1921 aatgctggta ttgggattag ttggtttctt ggtgtggatg ctgtcaatta tttatattgg
     1981 attttcagca accatacttt ggtcgtggtt tattgtgcct tttggcataa cgcctatcaa
     2041 tttggcgtgg gctgtcggcc tag
//
LOCUS       DADTFG010000301         2058 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.303, whole genome shotgun sequence.
ACCESSION   DADTFG010000301 DADTFG010000000
VERSION     DADTFG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.303"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..1083
                     /gene="ag43"
                     /locus_tag="J1138_004688"
     CDS             <1..1083
                     /gene="ag43"
                     /locus_tag="J1138_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBA8456355.1"
                     /translation="MLQAGAFNYSLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDY
                     DRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGTTPESSGSYGFVRLEGD
                     LLRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLW
                     ADIVAQGTRHSMKASSDNNDFRARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLS
                     LDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPV
                     NWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLSI
                     QAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            1202..>2058
                     /locus_tag="J1138_004689"
     CDS             1202..>2058
                     /locus_tag="J1138_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456356.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALPFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKT"
BASE COUNT          462 a          555 c          576 g          465 t
ORIGIN      
        1 atgctgcagg ccggggcctt taactactcc ctcaaccggg acagtgatga gagctggtat
       61 ctgcgcagtg aagaacgtta tcgtgctgaa gtccccctgt acgcctccat gctgacacag
      121 gcaatggact atgaccggat tctggcaggc tcacgcagcc atcagaccgg tgtaaacggt
      181 gaaaataaca gcgtccgtct cagcattcag ggcggccatc tcggtcacga taacaacggc
      241 ggtattgccc gtgggaccac gccggaaagc agcggcagct atggcttcgt ccgcctggag
      301 ggtgacctgc tgagaacaga ggttgccggt atgtctgtga ccgcgggagt atatggtgct
      361 gcaggccatt cttccgtaga tgttaaggat gatgacggct cccgcgccgg cacggtccgg
      421 gatgatgccg gcagccttgg cggatacctg aatctgacac atacatcctc cggcctgtgg
      481 gctgacattg tggcacaggg aacccgccac agcatgaaag catcatcgga caataacgac
      541 ttccgcgccc ggggccgggg ctggctgggt tcactggaaa ccggtctgcc cttcagtatc
      601 actgacaacc tgatgctgga gccacgactg cagtacacct ggcaggggct ctccctggat
      661 gacggtaagg acaacgccgg ttatgtgaag ttcgggcatg gcagtgcaca acatgtgcgt
      721 gccggtttcc gtctgggcag ccacaacgat atgacctttg gtgaaggcac ctcatcccgt
      781 gccccactgc gtgacagtgc aaaacacagt gtgcgtgaac tgccggtgaa ctggtgggta
      841 cagccttccg ttatccgcac cttcagctcc cggggagata tgcgtgtggg gacttccact
      901 gcaggcagcg ggatgacgtt ctctccctca cagaatggca catcactgga cctgcaggcc
      961 ggactggaag cccgtgtccg ggaaaatatc accctgagca ttcaggccgg ttatgcccac
     1021 agcgtcagcg gcagcagcgc tgaagggtat aacggtcagg ccacgctgaa tgtgaccttc
     1081 tgacagaacc atcgcctctc tgtggtcccg gtcatcatga ccgggccccg gacaggtgca
     1141 aacgcttcag taccacattc actggcattc acaataacat gatattcatc acggagtgac
     1201 tatgttacag atagtcggcg cgctgattct gctgatcgca ggatttgcca ttcttcgcct
     1261 tttgttcaga gcattaacca gcacggcgtc tgcgctggca ggattcgtat tgctgtgtct
     1321 gttcggcccg gctttactgg ctggctatat cactgaacgc ataacccggt tattccatat
     1381 tcgctggctg gcaggcgtat ttctgacgat tgctggaatg atcatcagct tcatgtgggg
     1441 acttgatggt aaacatatcg cactggaggc tcataccttt gactctgtga aatttattct
     1501 gaccaccgct ctcgccgctg gtctgctggc tcttcccgtg cagataagaa caattcagca
     1561 gaacgggctc acaccagaag atatcagcaa ggaaattaac gggtattact gctgttttta
     1621 tactgctttt ttccttatgg cgtgttctgc atacgcacca ttgatcgcat tgccgttcga
     1681 tatttcaccc tcactgatgt ggtggggcgg gttgttgtac tggctggctg cattagtgac
     1741 gctgctatgg gcggccagcc agatccaggc gctgaaaaaa ctgaccagtg ccatccgcca
     1801 gacactggaa gaacaaccgg tgctcaacag taaatcgtgg ctgagcagtt tgcaaaacga
     1861 ttacagcctt cctgaaacgt tgacggagcg catctggctc acgctcattt cacaacggat
     1921 ttcccgggga gaactgaggg aatttgaact ggcagacgga aactggctac tggacaatgc
     1981 ctggtatgaa agaaacatgg cgggtttcaa cgaaaagctg agagagagcc tgtcatttac
     2041 ccctgatgaa ctgaaaac
//
LOCUS       DADTFG010000302         2026 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.304, whole genome shotgun sequence.
ACCESSION   DADTFG010000302 DADTFG010000000
VERSION     DADTFG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.304"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..87)
                     /locus_tag="J1138_004690"
     CDS             complement(<1..87)
                     /locus_tag="J1138_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008039659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBA8456357.1"
                     /translation="MNILVTGGAGYIGSHTSLCLLNKGYNVVI"
     gene            complement(109..777)
                     /locus_tag="J1138_004691"
     CDS             complement(109..777)
                     /locus_tag="J1138_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA8456358.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNIEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVAIVFKDDNAVSFHEDVQEGVAEYITE
                     QNNY"
     gene            complement(774..1550)
                     /locus_tag="J1138_004692"
     CDS             complement(774..1550)
                     /locus_tag="J1138_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005183775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA8456359.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISNRSVGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVAVYILLMLIVWMAGEYFEITNFLQLVLTWSLLIILSCGVGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
BASE COUNT          615 a          466 c          356 g          589 t
ORIGIN      
        1 tattacaaca ttgtaacctt tattcagaag acataaacta gtatgcgagc caatatagcc
       61 tgctccacct gtaactaata tattcatgtt tcaccctcga aaacaacatc agtaattatt
      121 ttgttccgtt atatactcag caacaccctc ctgaacatcc tcatgaaaac taaccgcatt
      181 gtcatcctta aacacaatcg ccacatcaca aaactctttc aatgaattca agctatgtga
      241 aaccattaaa aaactagatt ctttatgcct ttctttaaac aattgagcgc atttttcttt
      301 aaacctggca tcaccgactg cggttacttc atcgacgata taataatcaa acttaaatgc
      361 catgcttaaa ccgaagccta aacgcgatcg cataccggat gagtaagttt taatcggcat
      421 atcaaagtac ttgcccagtt cggcaaattc ttcgacaaac tcaattttct ctttcagttc
      481 ttcttgcttt gcgtataacc gcgcaacaaa ttttacgttc tcgcgtccag ttaaacttcc
      541 ctgaaatcca ccggcgaggc cgactggcca tgatattgtt ttattggtga tgatctttcc
      601 actgtcgggg cggtcaatac caccaatcat tctcagtaac gttgatttac ccgctccgtt
      661 gcgaccaata aaggcaacac tttttcctga agggatttca atatttaaat ccttaaacac
      721 atagtgtcga cccgttggcg tgcgatatga cttcgtcaaa ttttcaatct taatcatgat
      781 gtcagcattg cctcttcacg cgttcggtat aacgccagac cgatgaacag ggtgaccaga
      841 gtgaacattg ccaggtagtt cagactcaca ccttcgctga tatagccagg cataactgcc
      901 tcgcggctta actccacgac atgcactaat gggttccaga gtagatacga ccagtattgc
      961 tttggaattg agtgcagagg gaacatgatg caggagatga aatacagcgg cttaagcagt
     1021 atcggcagga ccttttgcat ttcagggaag gttttaccaa cgaccatgaa tattaagcca
     1081 acgccacatg aaagaatgat taacaaactc caggtaagca caagttgtaa aaagtttgtt
     1141 atttcgaaat attcgcctgc catccagacg ataagcataa gcaatatata aacagcaacg
     1201 taaatcagtg tctcaagcaa tgcacgcgca atgatcgtat cgatgggttt tactggtcga
     1261 taattaaaca gcccctgatt cgcttcaata gcacctacag aacgattgct gatactgcta
     1321 aagataaaaa agggaatcag gccattaagt aaaaacaccg ggaatgagat gtctggcatc
     1381 gtgcggtgca taatgtaacc aaaaatgccc aacagtatca gcaaatgcgc agagggttcg
     1441 agaatcgccc acaaatatcc cagacggaac ttaccaaagc gtgttcgtat ttctcgtaga
     1501 aataatgcct ctacggtgac tttctggact tcaaatccac ttcttgccat ttgatgatgt
     1561 gatcctaatc tcttcaggta cgctaccgcc cttggcttaa cagctaccaa tacactggag
     1621 gtaatgctta tttgtaactg gataaaattt actcagacgg tttgaccttt ttaatagccg
     1681 caaaaaattt cagcagaaat tatttccgca gggataaata gatctatttc cgtctcgttt
     1741 cttctaatcc attcttcagg catgaatgta ttcggtcttc tggtaacttc agaaaactgg
     1801 ccgttttaga atgtagagtt aatttctcag gaaattattt ttgttgttac tgttaactac
     1861 ttgattataa taccccctat ggattttatg cgtaacaata cctgcaatct gtttcaaacc
     1921 agaacgcacg cgattgatta ccaataaatt gttcaccgag aaactatttc cctatttaaa
     1981 attcactcat gtacttctta tttatatcta cagccccctc tttaca
//
LOCUS       DADTFG010000303         2012 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.305, whole genome shotgun sequence.
ACCESSION   DADTFG010000303 DADTFG010000000
VERSION     DADTFG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.305"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(287..496)
                     /locus_tag="J1138_004693"
     CDS             complement(287..496)
                     /locus_tag="J1138_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012541172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBA8456360.1"
                     /translation="MPDDIDRDQAFNEQCLETLIEQNRFRPASTLSLYHCRFCGKDIP
                     AKRRQALPGVTTCINCQSILEKRRS"
     gene            complement(486..1070)
                     /locus_tag="J1138_004694"
     CDS             complement(486..1070)
                     /locus_tag="J1138_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HBA8456361.1"
                     /translation="MYPFLNYIMLTVVLRALREGNINYCEAMGFTHDEMNLIGKLSVP
                     ELISIGLLSTPFVDISVRHDILRQFLILSRNESRRHEQLNRAVRLGGSIALMNRYFGI
                     SSKEVCARRRLLGIAVPNGRTPTPDEETDAEIWNQWKKYQVENIDSPEALDAMMQVTE
                     NLSSCTDSPSLTVVWNCIMHYEREAAVRRIRRAR"
     gene            complement(1259..1444)
                     /locus_tag="J1138_004695"
     CDS             complement(1259..1444)
                     /locus_tag="J1138_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBA8456362.1"
                     /translation="MTSHQLLRLRQVEQKTGLKRSQIYLYMKKGTFPRSIKIGPSSVA
                     WLESEIDEWVNEKLSAR"
     gene            complement(1586..>2012)
                     /locus_tag="J1138_004696"
     CDS             complement(1586..>2012)
                     /locus_tag="J1138_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456363.1"
                     /translation="EKRVENQLGQLPKKAAMNVMAQLAAEKIMKYRQIDYFPLYNLPS
                     DACFVIRYAEVEKLINLYKKDIKSTKSSPRITTPLSRLFWLACKHNDTISPLLKHPYK
                     LLSIFEQWASDDGIKEELNAETLKRALERGSPSSTSLFS"
BASE COUNT          539 a          368 c          436 g          669 t
ORIGIN      
        1 tgtttgtggt tggattatga tgattaagtt ttatattcag gtggttaaag taagagtcga
       61 gagcgcatat tatctttatg cttccggggg cttcttgtat agttcttatt ggcatgtatc
      121 gcaataaata taagttagtg aaatattaag aagattctac cagttctgtg atatttatgc
      181 tcgaagataa tgaagtggct ctaaaataat ttagtgactc aactttaagt tcgtggtttt
      241 taattcgaaa taaaagtgcg gagtaaaact ccgcacgtac cggtgtctaa gatcttcttt
      301 tctcaaggat tgactgacag ttaatgcagg tggttacgcc tggaagtgct tgccgccgtt
      361 ttgctggaat atctttgccg caaaatcgac aatgataaag agaaagtgtt gatgcaggtc
      421 tgaatcggtt ctgctcaatt agcgtctcca gacactgttc attaaacgcc tggtcgcgat
      481 cgatatcatc tggcacgccg tattctcctt actgctgctt ccctttcgta atgcatgatg
      541 cagttccaga caacggtcag agaaggactg tcagtacatg atgaaaggtt ttctgtaacc
      601 tgcatcattg cgtccagagc ttcaggcgaa tcaatatttt cgacttgata ttttttccac
      661 tgattccaga tttcagcatc tgtttcttca tcaggagtgg gcgttctccc gtttggtacg
      721 gcgataccaa gtaaccgtcg acgtgcgcag acttctttag agctaatacc aaaataacgg
      781 ttcatgagtg caattgagcc accgagtctg acggctctat ttagctgttc atgacgtctg
      841 ctttcattgc gggataatat gagaaattgt cgtagaatat catgtcgaac actaatatcg
      901 acaaatggtg tggataaaag gccgatacta ataagttcgg gtactgataa ttttccaatc
      961 aaattcattt cgtcgtgagt gaatcccatt gcttcacagt agtttatgtt cccttcgcgt
     1021 agtgccctga gaactacagt tagcataata taattcagga acgggtacat agagtttgcc
     1081 ccttatttta aatgtcatta agaaagtcaa tctgattttc agtgtgggtt atgctgtgat
     1141 atagcctgga tacatgtctg tccttgttat actggtgtga gatttctttt ttggactgaa
     1201 aagtaggatt cataattgaa agcatatttt ttctcatttt tatctcctca taaagacttc
     1261 agcgagcgga taacttttcg ttgacccatt cgtcaatttc agattcgagc caggctacac
     1321 tagatggtcc tatttttatt gagcgaggaa atgtcccttt tttcatatat agatatatct
     1381 gtgagcgttt caaccctgtt ttttgttcaa cctgcctgag gcgtagtaac tgatgggatg
     1441 tcatatttcc tcctgtggat taaataaccg gcaaaccgga gattacaaaa aaacacagtg
     1501 aagtcccgaa ataaagcagg tgtcccctta ataagagtat gttcccctta ttaaggattt
     1561 tttgtcttaa cacatgagga tggtgttaac tgaataagga tgtagaggat ggtgacccac
     1621 gttccagagc tctttttaga gtttctgcat tcaattcttc tttgatgcca tcatctgatg
     1681 cccactgctc aaaaattgag aggagtttat aagggtgttt aagtaacggg ctgatagtgt
     1741 cattgtgctt gcaggccaac cagaataggc gagataaagg cgtggttatt ctcggggatg
     1801 atttggtgct ttttatatcc tttttataca gattaattag tttttcaact tcggcatagc
     1861 gaattacaaa gcaggcatca gagggtaagt tataaagagg gaaataatca atttgtctgt
     1921 atttcataat tttctccgca gcgagttgag ccataacgtt cattgcggct tttttaggaa
     1981 gttggcctaa ttgattttca actcttttct cc
//
LOCUS       DADTFG010000304         1996 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.306, whole genome shotgun sequence.
ACCESSION   DADTFG010000304 DADTFG010000000
VERSION     DADTFG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.306"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            85..333
                     /locus_tag="J1138_004697"
     CDS             85..333
                     /locus_tag="J1138_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456364.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIAQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            757..1524
                     /locus_tag="J1138_004698"
     CDS             757..1524
                     /locus_tag="J1138_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA8456365.1"
                     /translation="MRRAINNGEIVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISP
                     AAFIPLAEKCGLIVPLTQSLMNQVARQMNAIASKLPEGFHIGINFSASHIILPTFVDE
                     CLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLS
                     YLHDLHIDYIKIDHSFVGRVNADPASTRILDCVLDLARKLSISIVAEDVETKEQFDYL
                     NQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKIVVE"
     gene            1656..1874
                     /locus_tag="J1138_004699"
     CDS             1656..1874
                     /locus_tag="J1138_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456366.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
BASE COUNT          531 a          394 c          434 g          637 t
ORIGIN      
        1 aaaactcatt catcttattt ttgtctgtcg ctttagacta tacacagcat aattttattg
       61 gatcactatt cctgcgaggc tgacatgaat aattcaatcc cagagagatt tatttttcaa
      121 tgtgctttgt ttaaaaatct cgagagggag gtgttcatga cacatggata tgttgatagt
      181 catattattg ctcaggcttt acgcttgcgt ttaaaagatg agaccagtgt tattctttct
      241 gatctctatc tgcaaatctt gcagtacatt gaaatgcata agactacact aacggatatc
      301 attattaatg acagggaatc tgtgctctct taaaacttga gtaaggtttt tgaataaaca
      361 tcagctgtat cactatgctg atgcaaagtg agcatttcag gcgttatgct ttcttaatat
      421 atccgcaata tcagagggca aacaggaatg gagagcgctc gttttccatt cttgacgcct
      481 gatatcccgc ctaatttgtt taattctgcc tacattgtgc taaaaaatta ataggctaga
      541 tgaagttgga gaaaatttta tcttcggcgt ggatatttgt gatgctctct accatgctct
      601 ctgtagaccc cagcgcgagc gaatgaacgt ctctttagct tctacagact tatcgataaa
      661 ggctttggga ttttgatatt tattctgtta atcgcctgcg ccgctgcctt cctgcttgat
      721 aggtatttca ataaaagcgc aacgcctgaa gagatcctgc gacgggctat aaataatggg
      781 gagatcgtcc ctttttacca acctgtggta aatggtcggg aagggacatt gcggggagtt
      841 gaggtgttag cccgctggaa acaacctcac ggtggatata tatcacccgc ggcatttatt
      901 ccacttgctg aaaaatgcgg attaatcgtt ccgcttacgc aaagcctgat gaatcaggtt
      961 gccagacaga tgaacgctat cgcgagtaaa ctgccggaag gttttcatat tgggattaat
     1021 tttagcgcct cgcatattat tttgccgacg tttgtcgacg agtgcttaaa ttaccgtgac
     1081 agttttaccc gccgcgattt aaaccttgtt ctggaagtca ccgagcgtga gccattaaat
     1141 gttgatgaaa gtctggttca gcggttgaac attctgcatg aaaatggttt tgtcatcgcg
     1201 ctggatgatt tcggtactgg ttactcaggg ctttcttatc tgcatgacct gcatattgat
     1261 tatatcaaaa ttgatcacag tttcgttggc cgcgtcaacg cagacccagc atcaacccga
     1321 attctggatt gtgtattgga tctggcgcgt aaactttcga tcagtatcgt cgctgaagat
     1381 gtcgaaacga aagaacaatt tgactatctg aaccaaaata atatcacatt tcagcagggt
     1441 tattatttct ataaacctgt tacatacatc gacctggtca agattatcct ttctaaaccg
     1501 aaggtgaaga ttgtggttga gtgaaaattg atcagcaagg ccatggtgcg gtgtaattat
     1561 agacagctaa ttagctcgtt gcctcttgtt actattgttc attcttttgt ttgctataat
     1621 tgtgtgaaag ttttgactgg attgccatta gtagcatgaa caatagtaat aatctggatt
     1681 atttcactct ctatatcatc ttttccattg catttatgct gatcactctc ctggtcatcc
     1741 ttattgcaaa acccagcacc gggctgggag aagtgcttgt gacgataaat ttgcttaatg
     1801 ccctggtttg gctggcgatc aatctggtta atcgattaag ggaaagactc gtcagccaca
     1861 gggatcagca ctaatctttc agtttctcac tgccagtatg cggctgaatg ggttgctggc
     1921 agtgaacccc tggatcattg aaggaaagga attattgcac aaatagttgt caaccctggt
     1981 gttatcacag ttgttt
//
LOCUS       DADTFG010000305         1983 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.307, whole genome shotgun sequence.
ACCESSION   DADTFG010000305 DADTFG010000000
VERSION     DADTFG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.307"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>1983
                     /locus_tag="J1138_004700"
     CDS             <1..>1983
                     /locus_tag="J1138_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019455149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA8456367.1"
                     /translation="VKKNELNSATLSGIARSGGHITLYSALDARKTQEKVAQHPAYRL
                     AGEQVKQLVGEEGLDAAIAGLKTSLFTPAQQAIHLAIPTLEGDSVAFGQAKLLAGSLG
                     FAAKGVSMEAIQNEIEAQVKRGELLKVDVAPGVGADLLVSRASFEAEKSIIRLIADGK
                     DSLPPLVTTSSKTHNEDGPGQREGKVAPYLTQGQRQAVDLILNTTDRFVAIQGYAGVG
                     KTTQFKAVLDGIAQLPESERPRVVGLAPTHRAVGEMQAAGVDAQTLASFLHDEGLKVA
                     GGEKPDYRNTLFVVDESSMVGNSDMARTYALIAEGNGRAVMSGDEAQLEAIAPGAPFR
                     LAQGRSAIDMAVMKEIVRQTPELKPAVYKMIDNDVPGALDVVRSVAPANVPRQANAWV
                     PAKSVMELAVDIPDIHDAIVSDYLGRTEAARQDTLIITHLNADRHIINASIHGERKAA
                     GELKGESVSLPILETANIRDGELRKLETWARNRDAVVLLDSTYYRMAGIDSQSRTVSL
                     QDSEGNTRQFSPASAVREGVTLYNPRTIEVSVGDRMRFSKSDVERGHVANSTWTVEAI
                     KGDVVTLSDGQQTRTVNPREQQQDRHVDLAYAITAHGAQGASERFAITLEGVEGGREP
                     LVNRASAYVALSRAKEHVQVYTDNEAGWLNAIGKAKA"
BASE COUNT          423 a          560 c          670 g          330 t
ORIGIN      
        1 gtgaagaaaa acgagcttaa cagcgccacg ctgagtggca tcgcgcgcag cggcgggcac
       61 atcaccctct attcagcgct tgatgcgcgc aaaacgcagg agaaagtcgc ccagcatccg
      121 gcgtaccgtc tggcaggcga gcaggtcaaa caactggtcg gtgaggaggg gcttgatgcc
      181 gcgatcgccg ggctgaaaac atcactgttt accccggcgc aacaggccat tcatctggcc
      241 attccgacac tggaagggga tagcgtcgcg tttggccagg ccaaactgct cgccggcagt
      301 ctcggttttg cggccaaagg cgtgtcgatg gaggccatcc agaatgaaat cgaggcgcag
      361 gtcaaacgtg gtgagttgct gaaagtggat gttgcgcccg gcgtcggtgc ggacttgctg
      421 gtctcgcgcg ccagcttcga ggcggagaaa agcattattc gattgattgc ggatggcaag
      481 gacagcctgc cgccgttggt aacaaccagt agcaaaaccc acaacgagga cggccccggt
      541 cagcgggagg gaaaggtggc tccgtacctg acccagggac agcggcaggc tgtcgacctt
      601 attttaaaca caaccgaccg atttgtggca atacagggct atgcgggtgt ggggaaaacc
      661 acccagttca aggcggtact ggacgggata gcgcagttac cagaaagcga gcggccccgc
      721 gtggtcgggc tggcgccgac gcaccgcgcg gtgggtgaaa tgcaggcggc cggcgtcgat
      781 gcgcaaacgc tggcgtcgtt cctgcacgat gaggggctga aggtcgccgg cggcgagaaa
      841 ccggactatc gcaacacgct gtttgtggtg gatgagagtt cgatggtggg caacagcgac
      901 atggcgcgca cctacgcgtt gattgccgag ggcaacggcc gcgcggtgat gagcggcgac
      961 gaagcccagc tggaggcgat tgcgccgggc gcgccgttcc ggctggcgca agggcgcagc
     1021 gccatcgaca tggcggtcat gaaagagatt gtgcgccaga cccccgagct gaagccggcg
     1081 gtgtacaaga tgattgataa cgatgtgccc ggcgcgttgg acgtggtgcg ttccgtggcg
     1141 ccggccaacg tccctcgaca agccaatgcc tgggtgccgg caaagtcggt gatggaactt
     1201 gcagtagata ttccagatat tcatgatgcg attgtcagcg attacctggg gcgtaccgag
     1261 gcagcccggc aggatacgtt gattatcacc cacctcaatg ccgatcggca catcatcaat
     1321 gccagcattc acggcgagcg taaagcggcc ggggagctga agggtgagtc cgtcagtcta
     1381 cctatcctgg aaaccgctaa tattcgcgac ggcgagctgc gcaagctgga gacctgggcg
     1441 cgcaaccggg acgcggtcgt tctgctggat agtacctact accggatggc cggcatcgac
     1501 agtcagtccc gtaccgtcag cctgcaggac agtgagggca atactcggca gttttccccg
     1561 gccagtgcgg tgcgcgaagg cgtcacgttg tacaacccgc gcaccatcga ggtaagcgtg
     1621 ggcgaccgca tgcgcttctc caaatcggac gtcgagcgcg ggcatgtggc caacagcacc
     1681 tggacggttg aagcgataaa aggtgacgtg gtcacgcttt ctgatgggca acaaacccgt
     1741 accgtcaatc cccgcgagca gcaacaagac cggcacgttg atttggccta tgccatcacc
     1801 gcccacggtg cccagggggc cagcgaacgt tttgccatca cgctcgaggg ggtggaaggc
     1861 gggcgtgagc cgctggtcaa ccgggcgtcg gcctatgtgg cgctctcgcg cgccaaggag
     1921 cacgtgcagg tgtacaccga caacgaagcc gggtggctga acgccatcgg caaggcgaag
     1981 gcg
//
LOCUS       DADTFG010000306         1965 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.308, whole genome shotgun sequence.
ACCESSION   DADTFG010000306 DADTFG010000000
VERSION     DADTFG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.308"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            19..630
                     /locus_tag="J1138_004701"
     CDS             19..630
                     /locus_tag="J1138_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HBA8456368.1"
                     /translation="MQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIF
                     MQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYP
                     QYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRITKVNNETVS
                     YFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(596..721)
                     /locus_tag="J1138_004702"
     CDS             complement(596..721)
                     /locus_tag="J1138_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456369.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            735..1898
                     /locus_tag="J1138_004703"
                     /pseudo
     CDS             735..1898
                     /locus_tag="J1138_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
BASE COUNT          578 a          433 c          420 g          534 t
ORIGIN      
        1 tctatcggca gtaaacatat gcaatttatc gaattgctcg aaaccgggac gccgaatatt
       61 gatttcagtg gtcatagtga gaacacattc cgtatcattg atttttctgt gcctcccacg
      121 gcttatggca agtttatgtc aaccattttt atgcaatggg ttaaaaacga tgtgggtgaa
      181 attttcatcc gtcagtttga aagctttgtc agccgttttt tggggaatgg gcataccagt
      241 tgtattttcc aggagtcctg caaggataat ctggttgttg aaagtaatgg agacatttac
      301 gaatgcgacc attttgtcta tccacagtac aaaattggaa acattaataa atcggaactc
      361 aaaacgatga acagtgtaca actgacagcg caaaaaaaac ggatttcagc gaaatgtcag
      421 caatgtgtat ataaacctat ctgcaatggt ggttgcccta agcatcgtat tactaaagta
      481 aacaatgaga ctgtttccta tttttgcgaa ggttataaaa tccttttttc aaccatggta
      541 ccttatatga acgctatggt ggagttagct aagaacagag taccgcttta ccacattatg
      601 gatgttgcaa aacaaatgga gaataattaa taacgctccc ccttagctcc attcttagcc
      661 tccttcttat gaccttaaca aggcacccga cataaattcc tgtcgggtgt ttcgtcttca
      721 tcatcttcct ttgcgaaatt attgccgtat ctaataaagg cgatttaagt ttccctgcgc
      781 agcaaaaatc acttatcgcg ctggctaata aagcagtaag cggaagcggc gttggtgaac
      841 tctcctcttc cagcctgaaa cgctggagtg cggaaaactc ggtaaccatg agcagtaaag
      901 tcagtggcat gaatacgttg ctctccgtta gcgcgcggac taataaccct gaacctggtt
      961 tccagttgat taaccagcga atcacccaca gcacgattaa cgacaatatt tgggcatcgc
     1021 tacaaaatgc tcaaattcag gcgttgaaaa cgctcgacca gcgtccagcg gagaaattcg
     1081 cccagcagat gtatgagacg cgctatgccg atgaccgcac gaaattaccg caagaaaatc
     1141 agattgcaca gtttactgcc gcagatgcgc tggctgccga tcgccagttg ttttcatctc
     1201 cagcggatat cacgtttgtc attgtcggta atgtcgcaga agacaaactc gtggcgttaa
     1261 ttacgcgtta cttaggatca atcaaacact ctgattcgcc attagccgca ggtaaaccat
     1321 taactcgcgc gacggacaac gcatcggtta ctgtaaaaga acaaaatgaa ccagtggcac
     1381 aggtttcaca gtggaagcgt tataattccc ggactcctgt taatctggcg acgcgtatgg
     1441 cgctcgatgc ttttaacgtc gcactggcaa aagatctacg cattaatatt cgtgaacagg
     1501 catctggagc atacagcgtt tcttctcgcc tctcggttga tcctcaggcc aaagatatca
     1561 gtcatttgct ggcttttact tgtcaaccag aacgacatga tgaactgtta acgttagcga
     1621 atgaagtgat ggttaagcgt ctggccaaag ggattagtga gcaagaactg aacgaatacc
     1681 agcaaaacgt tcagcgcagc ctcgatatcc aacagcgtag cgttcaacaa ttagcgaata
     1741 ccattgtaaa tagtcttatt caatatgacg atcctgcagc atggactgag caggagcaat
     1801 tgttgaaaca aatgacggta gagaatgtta acactgctgt taaacaatat ctttctcatc
     1861 cggtgaatac ctataccgga gtattattgc caaaataatc acagccccgt caacacatcg
     1921 ttggcgggga ttttatcaat attcgctatt tttatgtaat aattt
//
LOCUS       DADTFG010000307         1958 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.309, whole genome shotgun sequence.
ACCESSION   DADTFG010000307 DADTFG010000000
VERSION     DADTFG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.309"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            730..909
                     /locus_tag="J1138_004704"
     CDS             730..909
                     /locus_tag="J1138_004704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456370.1"
                     /translation="MRHSTPTAMGIILGLLQGNATLATGKKTVYLSQNPQLMRTTKKW
                     THRDKWMFSNASAFI"
     gene            1022..1726
                     /locus_tag="J1138_004705"
     CDS             1022..1726
                     /locus_tag="J1138_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1120 domain-containing protein"
                     /protein_id="HBA8456371.1"
                     /translation="MMNAVKKTTCALAVLATTSTAVMAESIDVKVIGTITPTACKPTL
                     GGGGTIDYGNIPPASLSNDTFTVLAEKQVDFSITCDAPAKVAILAVGSKADTATNVTK
                     SPAGYDVFQGVLFAPSPGVVGLGVSGGKNIGGYGIRLVPGSYTVDGNQADTIGRKSDG
                     TWVPSANLWAGSLFTPAFKDLQISWASKGQLTPIAFTTAAGKLGVQAYITKTSELDLS
                     KPINLDGLTTLELVYL"
BASE COUNT          493 a          501 c          452 g          512 t
ORIGIN      
        1 tgtgcgcact gccgcctgcg gaaaagtcta tccagcgtgt gggagtgact ttagccctat
       61 cggctgtgat gtttcggagt aactggatct catcagtaat gcctttaagg cagtgtccaa
      121 agtttgacag ctaccggtgt gtgacctcat cgcccaggcc tcaatgccat cagcgcagct
      181 atgtggaccg ctggcactca tcgtaacggc gaagttcgcg gaacacacct ccaaccccga
      241 cgagacgtag aactaagtcg tgccgcgtta taacgcaggg catggcctaa caattaaggg
      301 aagcgcagac agtgatgtat cggaaaaaat tgtcagtcga cgcaagactg gatgatatct
      361 aatacccacg aacgtcaact ttgacgatat cggttttcat gatacggctc cttctgctac
      421 ggcaatatcg atttttatcg ccactttacc aatagctgcc ccacacttgt tcatgcctta
      481 cttaactaca gtagtttcga tatccaactt cccccttgta tcactatcaa gagccttaac
      541 tccttcaaaa tccaaagcag gattaatggt ctcaaaaaca aatcaataaa tgaattcaaa
      601 ccatcaaatt aaagatcaaa tgaatccata ttgactcatt gctgagggtg ttgtaacctc
      661 cacgccgttc cggtgagttg ccaaacatag ggtggtctgt gtggtttatg gaaaagggct
      721 gtttacggta tgcgtcattc cacaccaaca gcaatgggaa tcattttggg cttacttcag
      781 gggaatgcca ccttagctac tggcaaaaag actgtttatc tcagtcaaaa tcctcagctc
      841 atgcgcacca ctaaaaaatg gactcatagg gataaatgga tgttttctaa cgcctctgcg
      901 tttatttaat cacacgagat tgttgaccgc tggtgcaaat gttcctcaac gccaatcagg
      961 cggcttagag tacaaagtac catgcctatt taattactct tccgaatgaa tgagatataa
     1021 catgatgaat gcagtcaaaa agaccacttg cgcactcgca gtacttgcca ccacctccac
     1081 agctgtcatg gccgagagca ttgatgtgaa agtcatcggt actattaccc cgacggcttg
     1141 caaacctact ttgggggggg gaggtaccat cgactatggc aatattccgc ctgcatctct
     1201 tagtaatgat acatttactg ttctggcaga aaagcaggtt gattttagta tcacctgcga
     1261 cgcccctgcc aaggtggcca ttctcgccgt tggctcgaag gccgacaccg caacaaatgt
     1321 aactaaatca cctgctggtt atgatgtatt ccagggagtc ctcttcgctc caagtcccgg
     1381 tgtagtagga ctgggggtgt caggcggtaa aaatatcggt gggtatggca tcagattggt
     1441 accgggatcc tataccgttg acggcaatca ggcagacact attgggcgca aaagtgacgg
     1501 cacttgggtt ccctctgcca atctctgggc tggtagttta tttacccccg ctttcaaaga
     1561 cttgcaaatc tcttgggcct caaaaggtca gcttacccca atagctttca ctaccgccgc
     1621 tgggaaactg ggggttcagg cctacatcac caaaacctcc gagcttgacc tctccaagcc
     1681 catcaatctc gacggcttga ccaccctgga gttggtctac ctgtaatgct tctgccgacc
     1741 gggctcgcgc ccggtctttt ggtaactaat cgctgtttta gtttctttac gctttgacac
     1801 ggataatttc tcgaatgaat ctctctgtct tttgcaccgc tcccgtcgtt gctctgtggc
     1861 tgctttctgc tggagtccag gcctcgggca tgctgcccga gacgtcggtt gttatcgttg
     1921 aggaagaaga tggcgaaggt gccatcaatc tgcaaaac
//
LOCUS       DADTFG010000308         1912 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.310, whole genome shotgun sequence.
ACCESSION   DADTFG010000308 DADTFG010000000
VERSION     DADTFG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.310"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            66..1088
                     /locus_tag="J1138_004706"
     CDS             66..1088
                     /locus_tag="J1138_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HBA8456372.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1088..1867
                     /locus_tag="J1138_004707"
     CDS             1088..1867
                     /locus_tag="J1138_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA8456373.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          499 a          495 c          492 g          426 t
ORIGIN      
        1 gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag
       61 agcttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga
      121 gtagccgggc gattgccaga gaactgggga tctcccgcaa tacggttaaa cgttatttgc
      181 aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg
      241 aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa
      301 cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg
      361 cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg
      421 aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc
      481 acgtgttcgt tgctgttctc ggatacagcc gaatgctgta catcgaattc actgacaata
      541 tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc
      601 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
      661 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      721 cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg atggtgcagt
      781 acacccgtaa cagtttttac atcccactaa tgactcgcct gcgcccgatg gggatcactg
      841 tcgatgttga aacagccaac cgccacggtc tgcgctggct gcacgatgtc gctaaccaac
      901 gaaagcatga aacaatccag gcccgtccct gcgatcgctg gctcgaagag cagcagtcca
      961 tgctggcact gcctccggag aaaaaagagt atgacgtgca tcttgatgaa aatctggtga
     1021 acttcgacaa acaccccctg catcatccac tctccatcta cgactcattc tgcagaggag
     1081 tggcgtgatg atggaactgc aacatcaacg actgatggcg ctcgccgggc agttgcaact
     1141 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
     1201 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
     1261 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
     1321 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
     1381 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
     1441 ggcaatagcg atgggctatg aagcagtccg tgcaggtatc aaagttcgct tcacaacagc
     1501 agcagatctg ttacttcagt tatctacggc acaacgtcag ggccgttata aaacgacgct
     1561 tcagcgtgga gtaatggccc cccgtctgct catcattgat gaaataggct atctgccgtt
     1621 cagtcaggaa gaagcaaagc tgttcttcca ggtcatcgct aaacgttacg aaaagagcgc
     1681 aatgatcctg acatccaatc tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc
     1741 agcactgacc tcagcgatgc tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa
     1801 aggagaaagc tatcgactca gacagaaacg aaaggccggg gttatagcag aagctaatcc
     1861 tgagtaaaac ggtggatcaa tattgggccg ttggtggaga tataagtgga tc
//
LOCUS       DADTFG010000309         1884 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.311, whole genome shotgun sequence.
ACCESSION   DADTFG010000309 DADTFG010000000
VERSION     DADTFG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.311"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            115..1248
                     /gene="ompN"
                     /locus_tag="J1138_004708"
     CDS             115..1248
                     /gene="ompN"
                     /locus_tag="J1138_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBA8456374.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1389..1823
                     /gene="uspF"
                     /locus_tag="J1138_004709"
     CDS             1389..1823
                     /gene="uspF"
                     /locus_tag="J1138_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBA8456375.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          513 a          435 c          430 g          506 t
ORIGIN      
        1 ttacttaagg cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt
       61 tcactcccca aaatggggat gaaaggcaaa taaaataact aaaggattta ttcaatgaaa
      121 agcaaagtac tggcactctt aattcctgcc ctgctcgctg caggtgctgc acatgcagcc
      181 gaagtttata ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat
      241 tatttttctg ataattcagc gaaagatggc gaccagagct atgctcgtct gggttttaaa
      301 ggcgaaaccc aaattaacga tcaactcact ggctacggtc aatgggaata caatattcag
      361 gcaaacaaca ccgaatcttc aacaaaccag tcatggaccc gtctggcatt tgccggactg
      421 aaatttgcag attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc
      481 gaaggctgga ctgatatgct acctgaattt ggcggtgact cttataccaa tgcagacaac
      541 tttatgactg gtcgtgccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg
      601 gtaaatggtc tgaacttcgc ggtgcagtat caaggtaaca acgaaggtgc cagtaatggt
      661 caggaaggca ccaacaacgg acgtgatgtt cgccatgaaa acggtgacgg ctggggtctt
      721 tccacaacat atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac
      781 cgcaccaatg accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact
      841 gctgggctaa aatacgatgc taacaatatt tacctggcaa ccatgtattc agaaacgcgt
      901 aatatgaccc cgtttggcga cagcgattat gctgttgcaa acaaaaccca gaactttgaa
      961 gtcactgcac agtaccagtt tgattttggt ctgcgtccgg cagtctcttt cctgatgtct
     1021 aaaggccgtg acctgcacgc tgccggtggt gcagacaacc cagcaggtgt tgatgataaa
     1081 gatctggtta aatacgccga tgttggcgcg acttactatt tcaacaaaaa tatgtccacc
     1141 tacgttgact ataaaatcaa cctgttggat gaagatgaca gcttctacgc tgccaatggc
     1201 atctctaccg atgatatcgt cgctttaggt ctggtttatc agttctaaat ccccctgccc
     1261 gctgttttgg cgggcttttt ctgcctattc atcctccttt gatctaaatt aaaaatgtga
     1321 actccgtcat tacacaaaaa gtgtcatctg gcgttacact ttatgcggat actaaaacag
     1381 gaggttttat gaacagaacg attcttgtcc ctatcgatat ttccgattca gaattaactc
     1441 aacgcgtgat tagccacgtt gaggcagagg caaagattga tgatgcagag gttcatttcc
     1501 tgacggtaat accttcgctg ccctactatg cctctctggg tttagcgtat tccgcagaat
     1561 taccggcaat ggatgacctg aaagcggaag ccaaatcgca actggaagag atcattaaaa
     1621 aatttaaact gccaaccgac agagtgcatg tccatgttga ggaaggctcg cccaaagacc
     1681 gcattctgga attggcgaag aagatccccg ctcatatgat catcattgct tcccatcgac
     1741 cggatatcac cacttatctg ctcggttcta acgccgcagc tgtagtgcgt cacgcagagt
     1801 gctccgtgct ggttgtgcgc tgacactaac gcccgcacat tgctgcgggc tttttgattc
     1861 atttcgcaaa tgtgctgaca tttt
//
LOCUS       DADTFG010000310         1847 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.312, whole genome shotgun sequence.
ACCESSION   DADTFG010000310 DADTFG010000000
VERSION     DADTFG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.312"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            13..1017
                     /gene="argF"
                     /locus_tag="J1138_004710"
     CDS             13..1017
                     /gene="argF"
                     /locus_tag="J1138_004710"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBA8456376.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVIVATLAK
                     "
     gene            complement(1062..1514)
                     /locus_tag="J1138_004711"
     CDS             complement(1062..1514)
                     /locus_tag="J1138_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA8456377.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELRRAEYHARYLDIQIVLKGQEGMTFSTQPAGVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKA"
BASE COUNT          479 a          445 c          435 g          488 t
ORIGIN      
        1 caggagggat ctatgtccgg gttttatcat aagcatttcc tgaaattact cgatttcacg
       61 ccagcagaac tcaacagctt gttgcagtta gccgcgaagc tgaaagccga taagaaaagc
      121 ggtaaagaag aagccagact cactggcaaa aacatcgcgc tcatcttcga aaaagactcg
      181 acccgtaccc gatgctcttt cgaagttgcc gcatatgacc agggtgctcg cgttacttat
      241 ctcggcccaa gcggcagcca gattggtcac aaagagtcga ttaaagacac tgcccgcgtg
      301 cttggtcgca tgtatgacgg tattcagtat cgcggctatg gtcaggagat tgtcgaaaca
      361 ctggcggaat acgctggcgt gccagtatgg aacggcctga ccaatgagtt ccaccccacg
      421 cagctgctgg cggatctcct caccatgcag gagcatttgc ccggcaaagc attcaacgaa
      481 atgacgctgg tctatgcagg tgacgcgcgt aacaacatgg gcaattcgat gctcgaagct
      541 gcggcgctta ccggtctgga tttgcgtctg gtcgcgccac aggcatgctg gccggaagct
      601 gcgctggtcg ccgaatgcag tgctctggca caaaaacacg ggggtaaaat caccttgacg
      661 gaagatatcg cctctggggt taaaggtgca gactttatct ataccgatgt ctgggtatcg
      721 atgggtgagc ccaaagagaa atgggcggag cgtattgccc tgctgcgtga ctatcaggtg
      781 aatagcaaaa tgatggccct gaccggtaat tcgcaggtta agtttctcca ctgtctgcct
      841 gcatttcatg atgagcaaac cacgctgggt aaaaaaatgg ccgcagaatt cggtctgtat
      901 ggtggaatgg aagtcaccga tgaagtcttt gaatctccgg caagtattgt ctttgaccag
      961 gcagaaaacc ggatgcacac cataaaagct gttatagtcg ctacattagc aaaataacgt
     1021 tttgccgggc ggcggcacag gtcgcccccg gcagtgatca cttacgcttt taatagctta
     1081 actactgctt tccgtacctg agcgggggct cccaccgcac aaagcggttt gtgaacttcg
     1141 ccaggataga aaacgacaaa atccccttca ttaagaatca ctgttttttc atcgatcccc
     1201 tgcccgataa aagcaatatc tttatctgcc agccagtcag tctctggtac gcctgctggt
     1261 tgcgtgctaa atgtcatccc ttcctgccct tttaatacaa tctggatatc gagataacga
     1321 gcatgatact cagcacgacg cagttcacct ggttcagtcg tatcctcaga aatcagataa
     1381 aacaggcggt cgccgtcaat agcgtgctta ccttttgctg tctcatcgct gacatgtgat
     1441 ttgatgtact caattgcttc gcgaagttct tccggcagcc aggattgtaa gtgatggatg
     1501 ttgccaacaa tcataatatc ttcctttatt aaaacaatgt ttcacttctt tccttatacg
     1561 aaaaaaagcc acctctcacc atagaaaaat catcaggttt tgcccttgcg catgtttatt
     1621 gctggagatg ttgtactgat gttaagcagg agtaattttg tatgcagttt tgctgcataa
     1681 atcttataac ttagctatgt attttggtcc taatatcttg ttattgcata tgttaaaaga
     1741 aaactctcag tcagatcaca gttcattttc ttagctttta atgcccgtgc tgtcatagtt
     1801 atcccgtgaa aatagtgctg tgaaaaataa tcaccttatc ctttcgc
//
LOCUS       DADTFG010000311         1845 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.313, whole genome shotgun sequence.
ACCESSION   DADTFG010000311 DADTFG010000000
VERSION     DADTFG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.313"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..266
                     /locus_tag="J1138_004712"
     CDS             <1..266
                     /locus_tag="J1138_004712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA8456378.1"
                     /translation="MFLLYNWHPGNQLETQCQTEWYSALDTFFLRGQQLGIFRVDIAA
                     AAMSELLFSMLIGLQDAERRGRVAKMGLSRLMESVFLHGVVRV"
     gene            530..922
                     /locus_tag="J1138_004713"
                     /pseudo
     CDS             530..922
                     /locus_tag="J1138_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017891083.1"
                     /note="binds processed UmuD protein to form functional DNA
                     pol V (UmuD'2UmuC); involved in translesion
                     polymerization; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V subunit UmuC"
     gene            complement(969..1793)
                     /locus_tag="J1138_004714"
     CDS             complement(969..1793)
                     /locus_tag="J1138_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6) family putative aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBA8456379.1"
                     /translation="MVSYDPGCTASSPRYVTHTSQLLPVKTSTDGIKAMLKITDDLEE
                     QRGNALMAWWGGRGAAPVISHENEAILLERATGPASLSTMSREGQDTEACRILCLTAN
                     QLHIGQKQPIPQLTSLHDWFHALKPVARIYGGILKQCAEISEDLLSSPHDVTTLHGDL
                     HHANVLDFGSSGWLAIDPKGLKGERGFDYANIFTNPDLGNPASGIAVVPEIFKQRLNI
                     VTETAGLDRERLLMWICAWCGLSAAWSLESNDPVTVTTKIAELALSELEVKVNFCD"
BASE COUNT          440 a          454 c          476 g          475 t
ORIGIN      
        1 tgatgttttt gctctacaat tggcatcccg gtaaccaact tgaaactcaa tgtcaaactg
       61 agtggtattc agcgctggac acgttcttcc tgcgggggca acagctgggg atttttcgtg
      121 ttgatattgc agcagctgcg atgagtgaac ttttgttttc catgttgatt gggctccagg
      181 atgcggagcg tcgcgggcgt gtcgcgaaaa tggggctatc caggcttatg gaatcagtat
      241 tcttgcatgg tgttgttaga gtataagtaa gcgtaagtga gcgccgtatt gacggttatc
      301 tattcgtgag gtcaaggttc catagcagaa gttcagcaac tttattggaa ggccactccg
      361 gttacacact cagtatatga cgaaggcaaa atgttgcagg tgtaatattt ggcaaaaaga
      421 ttcaccaccg tacgccagca gcactgacgc tcatcagggc ctgatgaagt gcccgaccgg
      481 gtaaccggcg ggtcttcccc tccccacaca ctcaatgcga atccttttca tgctctcgat
      541 cacgactgca ctgcagcttg ccgacagcaa ccaggcgttg atgcgcagga cgttcggggt
      601 tgtgctcgag cgcaccgtgc gtgaactgaa ctgtgtgccg tgtatttcga tcgatgcgct
      661 gccggcaaag cagcagatca tcgtcagccg gtcatttggc cagcgcgtca cgcagctgca
      721 ggacatgcac caggcggtgt gtcagtacgc cgagcgggcc gccgaaaaac ttcgccgaga
      781 gaagcaatat tgccgacaca tttcggtgtt tgcacgaacc agcccatttg cacctcatga
      841 gccttattac gccaacagcc acaacgttca gcttatgttg gccacgcaag acacccgcga
      901 cattgttgcg gcggctgtga aaggggtcag tttggatatc gaaaattatt gtacgttacg
      961 gctgattttt aatcgcaaaa gttaacctta acttccagtt cagatagtgc cagctctgct
     1021 attttcgttg taacagtgac cgggtcgtta ctttccaaag accaagccgc ggataatcca
     1081 caccatgcgc agatccacat cagcagcctc tctctgtcca gaccggcagt ttcggtcacg
     1141 atattaagtc gttgtttaaa tatttccggg acaacggcta ttccagaagc tggattacca
     1201 aggtccggat ttgtgaaaat attggcatag tcgaaccctc gctcaccttt taagcctttc
     1261 gggtcaattg ccagccaacc agatgaccca aaatcaagga cgttagcgtg atgcaaatcg
     1321 ccatgtagcg tggtcacatc atgcggcgaa gatagcagat cttcagatat ttctgcgcac
     1381 tgtttcagaa tccccccata tatccgggcg actggtttaa gggcgtggaa ccagtcatga
     1441 agagaagtaa gttgtggtat tggctgtttc tgacctatgt gcaactggtt tgctgtaagg
     1501 cacagaatac ggcaagcctc tgtgtcctga ccttctctgg acatcgtgga cagagatgcg
     1561 ggccccgtgg cacgctccag aagaattgcc tcattttcat gggaaattac tggggcagct
     1621 ccacgtcctc cccaccatgc cataagtgca ttacctcttt gttcttccag gtcatcggta
     1681 atttttagca tcgcttttat gccatcggtt gatgttttaa ccgggaggag ttgtgatgta
     1741 tgggttacat atcgtggaga actcgcagtg cagcctggat cataagacac caggcagtgc
     1801 taaatcggat aatcatggcg ggctgatcgt ccagatatga aatat
//
LOCUS       DADTFG010000312         1792 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.314, whole genome shotgun sequence.
ACCESSION   DADTFG010000312 DADTFG010000000
VERSION     DADTFG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.314"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>1792
                     /gene="glyS"
                     /locus_tag="J1138_004715"
     CDS             <1..>1792
                     /gene="glyS"
                     /locus_tag="J1138_004715"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBA8456380.1"
                     /translation="AFDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLMYRAHVK
                     GQSAQQLLAGMVSTALGKLPIPKLMRWGDSDVQFVRPVHTVTMLLGADLIPGTVLGIE
                     SARTVRGHRFMGEAEFTLDNADQYPQILLERGKVVADYEARKALIKRDAELAAQKIGG
                     KADLSDSLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDAAGKL
                     LPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQ
                     QQLGTLRDKTDRIQALAGWVAGQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMG
                     MHYARHDGEAEDVAVALNEQYQPRYAGDALPQSLVACSLAIADKMDTLAGIFGIGQHP
                     KGDKDPFALRRAALGVLRIIVEKNLPLDLQTLTEEAVRLYGSKLTNANVVDDVVEFML
                     GRFRAWYQEEGHAVDTIQAVLARRPTKPADFDARVKAVSHFRTLDEAAALAAANKRVS
                     NILAKSTETLNDSVRASVLKDAAEIQLATHLVVLRDKLQPYFAAGNYQEALVELAALR
                     EPVDAFFDNVMVMADDAEVRVNRLTLLSKLR"
BASE COUNT          357 a          549 c          589 g          297 t
ORIGIN      
        1 ggcgttcgac gccgaaggca aaccgagcaa agcggccgaa ggctgggcgc gcggctgcgg
       61 catcaccgtc gatcaggcgg agcgcctggt gaccgacaag ggcgagtggt tgatgtaccg
      121 cgcccatgtc aaaggccagt cggcgcaaca actgctggcc ggcatggtca gcaccgcgct
      181 cggcaagctg ccgatcccga aactgatgcg ctggggcgat tccgacgtgc agttcgtgcg
      241 tccggtgcac accgtcacga tgctgctggg cgccgatctg atcccgggca ccgtgctggg
      301 catcgaatcc gcgcgcaccg tgcgcggcca ccgcttcatg ggcgaagccg agttcaccct
      361 cgacaatgcc gaccaatacc cgcagatcct gctggagcgc ggcaaagtgg tcgctgacta
      421 cgaagcgcgc aaagccctta tcaaacgcga cgccgaactg gcggcgcaga agatcggcgg
      481 caaggccgat ctgagcgaca gcctgctgga agaagtggcc tcgctggtgg aatggccggt
      541 ggtgctgacc gccaaattcg aagagaaatt cctggcggtg ccggcggaag cgctggtgta
      601 caccatgaag ggcgaccaga agtatttccc ggtgtacgac gccgcaggca aactgctgcc
      661 taactttatc ttcgtggcca acatcgagtc gaaagacccg cagcagatca tctccggcaa
      721 cgagaaggtg gtgcgtccgc gtttggccga cgccgagttc ttcttcaaca ccgaccgcaa
      781 aaaacgtctg gaagacaacc tgccgcgcct ggaaaccgtg ctgttccaac agcagctggg
      841 caccctgcgc gacaaaaccg atcgcatcca ggcgctggcg ggctgggttg ccggccagat
      901 tggcgccgac gtcaaccacg ccacccgcgc tggcctgttg tcgaagtgcg acctgatgac
      961 caacatggtg ttcgaattca ccgacaccca gggtgtgatg ggcatgcact atgcgcgcca
     1021 cgacggtgag gcggaagacg tcgccgttgc gctgaacgag cagtatcagc cgcgctacgc
     1081 cggtgatgcg ctgccgcagt cgctggtggc ctgctcgctg gcgatcgccg acaagatgga
     1141 caccctggcc ggcatcttcg gcatcgggca gcatccgaag ggcgataaag acccgttcgc
     1201 gctgcgccgc gccgcgctgg gcgtgttgcg catcatcgtc gagaaaaacc tgccgctgga
     1261 tctgcagact ctgaccgaag aggccgtgcg cctgtacggc agcaagttga ccaatgccaa
     1321 tgtggtcgac gacgtggtgg aattcatgct gggtcgtttc cgtgcctggt atcaggaaga
     1381 aggccacgcg gtcgatacca ttcaggcggt gctggcgcgc cgtccgacca aaccggccga
     1441 cttcgacgcg cgcgtcaagg cggtatccca tttccgtacg ctggacgagg cggcggcatt
     1501 ggctgcggcc aacaaacgcg tctccaacat tctggccaag tctaccgaaa cgctgaacga
     1561 cagcgtgcgc gcctcggtgc tgaaggatgc ggcggagatc cagctggcga ctcacctggt
     1621 ggtgctgcgc gacaagctgc agccgtattt cgcggcgggc aactaccaag aagcgctggt
     1681 ggaactggcc gcgctgcgtg agccggtgga tgcgttcttc gacaacgtga tggtgatggc
     1741 ggacgatgcg gaagtgcgcg ttaaccgtct gacgctgctg agcaaactgc gt
//
LOCUS       DADTFG010000313         1777 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.315, whole genome shotgun sequence.
ACCESSION   DADTFG010000313 DADTFG010000000
VERSION     DADTFG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.315"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            63..794
                     /locus_tag="J1138_004716"
     CDS             63..794
                     /locus_tag="J1138_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBA8456381.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAALLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            1015..1419
                     /locus_tag="J1138_004717"
     CDS             1015..1419
                     /locus_tag="J1138_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBA8456382.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            1472..1597
                     /locus_tag="J1138_004718"
     CDS             1472..1597
                     /locus_tag="J1138_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456383.1"
                     /translation="MEIKVQRLSLWMINTVFLLLPINNHQTNTIKLIFEMCSLDF"
     gene            complement(1681..>1777)
                     /locus_tag="J1138_004719"
     CDS             complement(1681..>1777)
                     /locus_tag="J1138_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HBA8456384.1"
                     /translation="AKTVTYDFERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT          530 a          379 c          383 g          485 t
ORIGIN      
        1 ttgtacaaag tattattggg tcgtgtagag gcgacggaga tttatgaccg caaggaggaa
       61 ttgtggctta ttacagcatt ggtgatgttg ctgaacgttg cgggattaat cctgtcactc
      121 tccgggcctg gcaacgccgc tacggtttgt taaaaccaca gcgcagtgaa ggcggacacc
      181 gactctttga tgaagaagac atacaacgca tcgaagagat caagcgttgg ataagtaatg
      241 gcgttcctgt aggcaaagtt aaagcattac tggaaactac cagccaggat acgcaagatg
      301 actggagccg cctgcaagaa gagatgatgt caattcttcg tatggctaat ccagccaaac
      361 tacgcgcgcg aattatttca ctgggtcgag agtacccagt agatcaattg attaatcatg
      421 tttaccttcc tgttcgccag cgtctcgtgc ttgatcacaa cacctcccgc attatgagca
      481 gtatgtttga cggcgcatta attgaatacg cagcggcctt acttttcgaa atgcgccgta
      541 agcccggtaa agaggccatt ctgatggcgt ggaatgttga agagagagca cgattgtggc
      601 tggaagcatg gcgtttatca ttgtcaggat ggcacatttc tgttcttgct gatcccattg
      661 aatcgccgcg cccggaactg ttcccaacgc aaacattgat tgtctggaca ggcatggcac
      721 caacgagaag gcaaaacgaa cttttacaac actggggtga gcaaggttat aaagtcatct
      781 ttcatgcccc ctaaattcac ctttatcccg ccccttagac agaatgttat aatctgaacc
      841 cacaccagcg tttgtagcgg agctggtccg ttgcaaaatg cccaccggga gaattcaggt
      901 aatccccctt tcacactttg tacatatcac agccaaagga cagaccagat tgaagtggta
      961 aaaccgatcg tgcacactac gcagtctcac acctaacaag aggttattat cctgatggat
     1021 caggtcgttg tttttcaaaa gatgtttgag caagtgagaa aagagcaaaa cttctcctgg
     1081 ttttattcag aattaaaaca tcaccgtatt gcacattaca tttattatct ggctacggat
     1141 aacatcagaa ttattactca cgatgacacg gttttgttat taagaggaac caggaacctg
     1201 ttaaaagtta gtacgaccaa gaacccagct aaaataaaag aggccgcatt gcttcatatt
     1261 cgcggaaaat ctacatttcg ggaatactgt tcaacactgg caggcgctgg cgttttccgg
     1321 tgggttactg atgttaatca taacaaacgc agttactacg ccattgataa tacgctttta
     1381 tatattgaag atgtagaaaa taataaacca ttaatctagc taaagttggg agcttaaaaa
     1441 atgcttcata attcagtaag gcattagcat aatggaaata aaagtgcaga gactatccct
     1501 atggatgatt aatactgtct ttttattgct ccccataaat aatcaccaga ccaatacgat
     1561 caaattgata tttgaaatgt gttcacttga cttttgatac gttattttat aacggttaac
     1621 atatttataa aaacaacggg cgtgttatac gcccgtttca atatttaaca catgtagtga
     1681 ttacatattc ttgatgatcg catcaccaaa ctctgaacat ttcagcagtt tagcgccttc
     1741 catcagacgt tcgaagtcat aggttacggt cttcgca
//
LOCUS       DADTFG010000314         1765 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.316, whole genome shotgun sequence.
ACCESSION   DADTFG010000314 DADTFG010000000
VERSION     DADTFG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.316"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..289
                     /locus_tag="J1138_004720"
     CDS             <1..289
                     /locus_tag="J1138_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456385.1"
                     /translation="NIIDSRNISAPHELANILTREKILTTREKKGNLISLFDGFTLFH
                     CVGMITFLIHYLRTPEEKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
     gene            complement(477..1679)
                     /locus_tag="J1138_004721"
     CDS             complement(477..1679)
                     /locus_tag="J1138_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8456386.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT          499 a          444 c          340 g          482 t
ORIGIN      
        1 taacattata gacagcagaa acatatcagc cccccatgaa ctggcaaata ttcttacccg
       61 cgaaaaaata cttacaacac gggaaaaaaa aggaaacctg ataagcctgt ttgatggatt
      121 cactttattc cattgtgttg gaatgataac ctttcttatc cattatctca gaacacctga
      181 agaaaaagtt gaaaatatat ttatgttata tggtgcagat aaaaacaata aactacgcag
      241 aagactgatt tatgacgcac taggaataat tcagtctcag caggagtgaa acgttaaaac
      301 aggcaaattg ttttttataa acaggagatt gttgagtaat ctcctgttta taaaaatacg
      361 ctttcctgac gatctgataa ttatcagctc aatacatttg acataaaagc ggttccttaa
      421 ttactactgc cacgatcaca tatcaaatca tgatcgtaca atatacattt tcactattat
      481 ttattcacaa tttgagacat cacataatcc gcccatgcct gcatcatcgg tacacgctgc
      541 tccagtagat cagtacgatg atatgccgcc tcaaccttat ttttcagcgt atgagcgagc
      601 gccctttccg ccagatcccg cgaatacccc tgttcactac accagtccct gaatgttgag
      661 cgaaaaccat gtgctgtggc aactcgcccc ggaatgtcac tgacggcttt ctttttacgc
      721 agaaaacttg tcaacaccat atcggaaagg atctgctgct ttctgggtga agggaacacc
      781 agttcatcat gcaggccacg tatattttcc agaatgtaaa tagcctgccg ggataaagga
      841 acacgatgct gtagcctagc tttcattctt tctgcaggta tagtccatac ccgcttatga
      901 aaatcaattt cagcccagcg cattcccctg gcttcgcccg agcgagttgc tgtaagtatc
      961 accattaata acagtgcgcg ggtaacatta taaggttcat cactatacac actggtcgcc
     1021 acaaaaagcg gtaactgcct ccagggcatt gcgggttggt gttcatcacg tcctcttgtc
     1081 tgctgaggaa gcaaatggtc aaccacatca acaggatttg ccacacaaaa accgtgcgcc
     1141 catccccact gcataacaac atgaatgcgc tgtttaaccc ggcttgccgt ttctgacaag
     1201 gttaaccaga ctggacgcag tgtttctgcc acgtccgcag ccgtaatcga atccagcgtt
     1261 tttgctccca gttgaggaaa cgcgtaattc tcaagcgtcg ataaccactg ccttacatgc
     1321 tttggatttt cccatccagg agacagttct gcatgtacac gcctggctgc atcggcaaat
     1381 gttgggataa cgactttctc agattcagcc tttttaatct ccagaggatc atcacctgca
     1441 gcaagttgct ctcgcattat ccgggcagta cgtgcagctt cagcaatact gacctctggg
     1501 taagttccca atccagcatt acgtcttttt tgtgtcaccg gacttacata acgaaaaacc
     1561 catttccccc gcccctttac tgaagaagga tgaagggtca gtccggtaat tcccccatgg
     1621 ggcaatggtt tgtcatcagg tttgatatgt cttgctttcg tatccgtcaa tactgccata
     1681 cgctaattcc cgtcactctg gtatgccatc cagtatgcca ttagtccctc gcttcactaa
     1741 gaatacatta gataatatcg gacaa
//
LOCUS       DADTFG010000315         1724 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.317, whole genome shotgun sequence.
ACCESSION   DADTFG010000315 DADTFG010000000
VERSION     DADTFG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.317"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..324
                     /locus_tag="J1138_004722"
     CDS             <1..324
                     /locus_tag="J1138_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA8456387.1"
                     /translation="LTTLFAAENHCLSPSEISQKLQFTRTNITRITDFLEKTGYVKRT
                     DSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWGYLTHDEQELFEVINKKLLAHL
                     DDVSS"
     gene            complement(650..1687)
                     /locus_tag="J1138_004723"
     CDS             complement(650..1687)
                     /locus_tag="J1138_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc40 family transposase"
                     /protein_id="HBA8456388.1"
                     /translation="MLSNMKIFITEQQKAELERLHDSSRDGRVRDRIKAILLASEGWS
                     SAMIAQALRLHQTTIDHHISEFLNKGKLKPENGGSDSKLSAEQTAFLISQLSDNLFHH
                     TRDVIAFVTRTWNIIFSIPGMNKWLHRNGVTYKKPSGVPHKFSEEKQRQFIEYYKELK
                     TTVGDEPILFIDGVHPTQATKISYGWIRKGQKKAVKTTGSRTRLNIMGALNLKALTSP
                     LICEYKTINEYNVSRFFNEIRKVYPDYNQKIHVILDEAGYHRSQLVKDWAEVVNIRLH
                     YLPPYSPNLNPIERMWKLMNEHARNNRYFSSTREFREAISVFFNQTLPNIADSLTSRI
                     NDHFQMLTPAS"
BASE COUNT          488 a          344 c          379 g          513 t
ORIGIN      
        1 ctcaccacat tattcgcagc ggagaaccat tgtctgtcac cttcagagat aagccagaaa
       61 cttcagttta ccagaactaa tattacccgc attacagatt ttttagaaaa aaccggatat
      121 gtaaaaagga cggatagcag ggaggatcgc cgtgctaaaa aaattagtct gacatctgaa
      181 ggtatgtttt ttattcagag gctcactctt gcacaaagca tgtatctgaa agaaatctgg
      241 ggttatctga cccatgatga acaggaactg tttgaagtca ttaataaaaa attactggcg
      301 catcttgatg atgtcagctc ataaagtgtg aaatgcctga cttgttgaaa gcagtagtgt
      361 tgcagaaaga cctggtgatg ctgatcttat atacataagc agcgcccgtg tctgaaatgc
      421 aggttagaaa caatgtgaac actatccgtt agaaattgtg acaaaattcc cgatgatcga
      481 caagcgcacc gattttccag tggcaacatc agtacaaaag atgaacggta ctgactttgc
      541 cggaatgagt caaaaagaaa gctgaactgg cactactagc agtgcttcag aaatttattt
      601 gactgaagta caatgaaata aaaaacatat gttatatact caataaacct caagatgcgg
      661 gtgtgagcat ctgaaagtga tcgttaattc gggatgtcag tgagtccgct atattcggca
      721 gtgtctggtt aaaaaagaca gatattgctt ccctgaactc tcgcgtactg ctgaagtagc
      781 gattattccg ggcatgttcg ttcattaatt tccacattct ttctatcgga ttcaggtttg
      841 gactgtaagg aggaaggtaa tgcagcctga tatttaccac ttccgcccag tctttcacta
      901 actgtgaacg gtggtaacct gcttcatcca gaataacgtg gattttttgg ttataatcag
      961 gataaacctt tcgtatctca ttgaaaaaac gggatacatt atattcatta atggttttat
     1021 attcgcagat tagcggtgac gtcagtgctt tcagattcag cgcccccatg atattcaggc
     1081 gtgtgcggct gcctgttgtt tttactgctt ttttctggcc tttgcgtatc cagccatagc
     1141 tgattttagt ggcctgagtc ggatggactc catcaataaa aagaatcggc tcatcaccca
     1201 cagttgtttt cagttcctta tagtattcaa tgaattgcct ttgtttctct tcactgaatt
     1261 tatgggggac gcctgacggt tttttgtagg tgacaccatt acgatgcagc cacttattca
     1321 tccccggaat gctgaaaatg atgttccatg tccgggtgac aaacgcaata acatcgcggg
     1381 tatggtgaaa taaattatcg gataactggc tgattaaaaa ggccgtttgt tcagcactga
     1441 gtttgctgtc tgagccgccg ttttcgggct tcagttttcc tttattcagg aattcgctga
     1501 tatggtgatc aatcgttgtc tgatgcaggc gtaaagcctg agcaatcatg gcagaactcc
     1561 agccttcgga agccagaaga atggctttaa tacgatctct gactcgcccg tcacgactgg
     1621 aatcatgaag gcgttcaagt tcagcttttt gttgctcagt aatgaatatt ttcatgttgg
     1681 acagcatgat cttctgttgc tgaaaaatca agcatcttca atga
//
LOCUS       DADTFG010000316         1717 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.318, whole genome shotgun sequence.
ACCESSION   DADTFG010000316 DADTFG010000000
VERSION     DADTFG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.318"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>1717
                     /locus_tag="J1138_004724"
     CDS             <1..>1717
                     /locus_tag="J1138_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA8456389.1"
                     /translation="PATGSYTVASETAKSRGKKSGRSKLLISALVAGGLLSSFGAYAE
                     VSLDGGTSAETPALDNVWIAIGKGSKASSVSAGGTASVAIGVQAEANVGSTALGFKSL
                     ATGEKSVSIGQLAIASGSRGVAIGSAANASASNTIAMGLLAKAGGDGSKQGAMAMGAY
                     SEANGSYSIAFGDHAKSQGDYGISLGETAGANGLYSLALGKESNAIGDDSLALGRLSL
                     ASAANAIAMGADSSSSNDNAIAIGNKTQALGVNSMALGNASQASGESSIALGSTSEAS
                     EQNAIALGQGSIASKVNSIALGSNSLSSGENAIALGEGSAAGGSNSLAFGSQSRANGN
                     DSVAIGVGAAAATDNSVAIGAGSTTDASNTVSVGNSTTKRKIANMAAGAISSTSTDAI
                     NGSQLYTISDSVAKRLGGGATVGNDGSISAVSYALKSGIYNNVGDALSGIDNNTLQWN
                     KTAGAFSANHGANATNKITNVAKGTVSATSTDVVNGSQLYDLQQDALLWNGTAFSAAH
                     GTEATSKITNVEDGTISDTSKDAVNGSQLKETKDDVATNTANIADNTADIATNTANIA
                     DNTADI"
BASE COUNT          485 a          390 c          479 g          363 t
ORIGIN      
        1 tccggcaaca ggcagttaca ccgttgccag cgaaacggcg aagagccgtg gtaaaaaaag
       61 cgggcgcagt aagctgttaa tttctgcact ggttgcgggt gggttgttgt cgtcatttgg
      121 agcatatgcc gaagtatcac ttgatggtgg tacaagtgct gaaacgcccg cgcttgataa
      181 tgtctggatt gctataggta aaggttcgaa ggcctcatca gtgtcagcag gaggaactgc
      241 gagtgttgcc attggggtac aagctgaagc aaatgtaggg agtacagcat taggttttaa
      301 aagcttagct acgggtgaaa aatcagtatc tataggacaa ttggcgatcg cttcaggaag
      361 tcgaggtgtc gcaataggaa gtgcggcgaa tgcttccgca tcaaatacta ttgctatggg
      421 tctattggct aaagctggcg gtgacggctc caagcaaggt gctatggcaa tgggcgcata
      481 ctcagaggca aatggcagtt actctatcgc ttttggcgat catgcgaaga gtcagggcga
      541 ctacggaata tctcttggag aaactgccgg ggccaatggc ctatatagcc tggcactagg
      601 taaagaaagt aatgcaatcg gtgatgacag cctggcatta ggcagattat cacttgccag
      661 tgcagcgaac gctattgcga tgggggcaga cagtagcagt tcgaacgata acgccatcgc
      721 gattgggaac aaaacgcaag ccctgggagt gaattcgatg gccctgggta atgcaagtca
      781 ggcatctggc gaatccagta ttgcattagg tagcaccagt gaggccagcg aacaaaatgc
      841 gattgcgctg gggcaaggta gcattgcaag caaagtgaac tcaatcgcgt tgggaagtaa
      901 cagtttgtcc tcgggagaga atgccatcgc attgggagag ggtagtgccg ctggtggcag
      961 caacagcctt gctttcggta gccagtccag ggcaaacggc aatgattctg tcgccatcgg
     1021 tgtaggggct gcagcagcga ccgacaattc tgtcgctatc ggcgcaggat cgaccacaga
     1081 tgcaagcaat acagtttcag ttggcaacag cacaacaaaa cgcaaaattg ctaatatggc
     1141 tgctggtgcc ataagcagca ccagtaccga tgccatcaac ggctcacagc tttatacgat
     1201 cagtgattca gtcgccaaac ggctcggggg aggcgctact gtaggcaacg atggcagcat
     1261 aagcgcagta agctacgcgt tgaaaagtgg catctataat aacgtgggtg atgctctgtc
     1321 aggaatcgac aataataccc tacaatggaa taaaaccgcg ggggcattca gtgccaatca
     1381 cggtgcgaat gccaccaaca aaatcactaa tgttgctaaa ggtacggttt ctgcaaccag
     1441 caccgatgta gtaaacggct ctcaattgta cgacctgcag caggatgccc tgttgtggaa
     1501 cggcacagca ttcagtgccg cacacggcac cgaagccacc agcaaaatca ctaatgttga
     1561 agatggtact atttcagaca ccagtaaaga tgcggttaac ggttcccagt tgaaagaaac
     1621 caaagacgat gtggccacca acacagccaa tatcgccgat aacactgctg atattgcaac
     1681 caatactgcc aatatcgccg ataacactgc cgatatc
//
LOCUS       DADTFG010000317         1687 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.319, whole genome shotgun sequence.
ACCESSION   DADTFG010000317 DADTFG010000000
VERSION     DADTFG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.319"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            83..298
                     /gene="essD"
                     /locus_tag="J1138_004725"
     CDS             83..298
                     /gene="essD"
                     /locus_tag="J1138_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBA8456390.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            303..614
                     /locus_tag="J1138_004726"
     CDS             303..614
                     /locus_tag="J1138_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HBA8456391.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            611..1144
                     /locus_tag="J1138_004727"
     CDS             611..1144
                     /locus_tag="J1138_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA8456392.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1141..1638
                     /locus_tag="J1138_004728"
     CDS             1141..1638
                     /locus_tag="J1138_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HBA8456393.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          489 a          346 c          437 g          415 t
ORIGIN      
        1 acaacgtgtg ctgtcaaaac taccccgtag actccgatct tttcaaacat attgcaccat
       61 ccgtgtacat cggggtgagg atatgaaatc aatggataag ttaacaacag gtgttgccta
      121 tggcacatcg gcgggtaatg ctggtttctg ggcattgcag ttactcgata aagtaactcc
      181 gtcacagtgg gctgcaatcg gtgtgctggg tagcctggtt tttggcctgc tgacgtatct
      241 gacaaatctt tatttcaaga ttaaagaaga caggcgtaag gctgcgagag gagagtaatc
      301 caatgactca agactatgaa ctggttgtga aaggagtccg taattttgag aataaagtta
      361 cggtaactgt agccttacag gacaaagaac gctttgacgg tgaaattttt gacctggatg
      421 tcgccatgga ccgtgttgaa ggagctgcgc tggagtttta tgaggcagca gccagaagga
      481 gcgtccggca agtcttcctg gaagtagcag aaaaattgtc agaaaaagtt gagtcttatc
      541 tgcagcatca gtactccttt aagattgaaa atcctgccaa taagcacgag cgtcctcatc
      601 ataaatatct atgaacacaa aaatcagata cggcctgtcg gctgccgttc tggcgctgat
      661 tggtgctggc gcatctgctc ctcagatact tgaccagttt ctggacgaaa aagaaggtaa
      721 ccacacaatg gcataccgcg atggttctgg catatggacc atctgtcggg gtgccacagt
      781 ggtggatgga aaaaccgttt ttcccaatat gaaactgtcg aaggaaaaat gcgaccaggt
      841 caacgccatt gagcgtgata aggcgctggc atgggtggag cgcaatatta aagtaccact
      901 gaccgaacca caaaaagcgg gtatcgcgtc attttgtccc tataacattg gccccggtaa
      961 gtgtttcccg tcgacgtttt ataagcggct gaatgctggt gatcgtaaag gtgcatgcga
     1021 agcgattcgc tggtggatta aggatggcgg acgcgattgc cgcattcgtt caaataactg
     1081 ttacggtcag gttattcgtc gtgaccagga gagcgcatta acctgctggg ggatagaaca
     1141 gtgaatcaga tattcatggt gatttttctc gtgttgtcag gatttatcgt cggaaatgtc
     1201 tggagcgaca gaggatggca aaaaaaatgg gcggaacgtg atgctgccgc attatcacaa
     1261 gaggtaaatg ctcaatttgc tgctcgaata attgaacagg ggcgaactat agcccgtgat
     1321 gaggctgtta aagatgcaca acagaaatct gctgaaattt ctgccagggc tgcttatctg
     1381 tctgatagtg ttaaccagtt gcgtgccgaa gcaaaaaaat atgccatacg ccttgacgca
     1441 gcgaagcata ccgcagatct tgccgctgcc gtcagaggca aaacaaccaa aaccgccgaa
     1501 ggaatgctca ccaacatgct cggagatatt gcagcagaag ctcagcttta tgctgaaatt
     1561 gctgacgaac gctacatcgc aggagtgact tgtcaacaga tctatgaatc tttaagagat
     1621 aaaaagcatc aaatgtaggg taatattaaa tcggaacatt tacatcgcgg aatgtaaaat
     1681 ttaaata
//
LOCUS       DADTFG010000318         1669 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.320, whole genome shotgun sequence.
ACCESSION   DADTFG010000318 DADTFG010000000
VERSION     DADTFG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.320"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            435..1382
                     /locus_tag="J1138_004729"
     CDS             435..1382
                     /locus_tag="J1138_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019455153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein"
                     /protein_id="HBA8456394.1"
                     /translation="MASKVVARMSSHDWRRNDDLIGLRRQGYIPYTQRNNPDYRPKPM
                     RIAARSESREALTVLSMVLGANCDYNPDSDYPFEIMLPFEDVAKAMGVLHVYESGRKA
                     YDVALHALSVLEQLDYLIVSRGQDTDTGQNKPLRIWLTENFFTSRGIRVDEIRQWLNQ
                     YRLWAIKNGLTETLRQKYERHLVRIAHLGIDIERKHSLKNRLKKIRRWVVSPDLQNLK
                     RNAEQVIGNELARRQQNEQRLDTLLDDTAAGIKKLAAARRQKQNGFYQAWVQWTMGSS
                     PLKAMQLENTLKHEQPGLLNSDPEAFYRLLLERAGAIPA"
BASE COUNT          379 a          463 c          493 g          334 t
ORIGIN      
        1 tgctttgtta cttgaccagg tgacgatcgc cacggccgcg gggcgcgttg tccgctttac
       61 cgatatgaag gcttttggta gagtgggaaa aattttgcat tcgatggttg tccacgccag
      121 aaggtggacg ggatggagcg gagctccaga cgcaaaaacc cccggattac gcatgaactt
      181 tggtcggtgc atcgtaaatc cgggggtcac aagcaggcct ggcgcctgta aggaagtata
      241 ctgtatgcgt attctacgtc aatcccccct gccgacccta agtgcagggg aacgtgcata
      301 gtcaacatgc cgcgcaggta aatcctgtaa aatcaaccat ttcacgccgt ggtgccaata
      361 atgcgctgcg gcgcgggcaa caaaaaattc atcgtcgtta caccccaaac ctgtgctgcc
      421 gggtgccgaa aggcatggcg agcaaggtgg tggcgcgcat gagcagccac gactggcggc
      481 gtaatgatga cctgatcggc ctgcgccggc agggttatat cccttatacc caacgcaaca
      541 acccggacta ccgtcccaaa ccgatgcgca ttgctgcgcg ttcggagagc cgtgaagcgc
      601 tgacggtgct ctcgatggtg ctgggcgcca actgcgacta caacccggac agcgactacc
      661 cgttcgagat catgctgccg ttcgaggacg tggccaaagc gatgggggtg ctgcacgtct
      721 acgaaagcgg ccgcaaggcg tatgacgtgg cgttgcatgc gctgtcggtg ctcgagcagc
      781 tggactatct catcgtgtcg cgcgggcagg atactgacac cggtcagaac aagccgctgc
      841 gcatctggct gacggagaac ttcttcacaa gccgtggcat ccgggtggac gaaattcgcc
      901 agtggctgaa tcagtaccgg ctgtgggcga ttaaaaacgg cctgaccgaa acgctcaggc
      961 aaaagtacga gcgccacctg gtgcgcatcg cgcatctggg gatcgacatc gagcgcaagc
     1021 actcgctgaa aaaccggctg aaaaagatcc ggcgctgggt ggtgagtccc gatctgcaaa
     1081 acctcaaacg taacgcggag caggtgattg gcaacgaact ggccaggcgg cagcagaacg
     1141 agcagcggct cgatacgttg ctggatgaca ccgctgcagg catcaaaaag ctggccgccg
     1201 cccggcgcca gaagcagaac ggcttttatc aggcctgggt gcagtggacg atgggcagct
     1261 cgccgctgaa ggcgatgcag ctggagaata cgctcaagca tgaacagccc ggactgctga
     1321 acagcgatcc ggaggcgttc tatcgcttgc tgctggagcg cgccggcgct atccccgcct
     1381 aacttcctcc cacatacaga atttttcccc tgtctgatcc gcgggggttt tgtgctgcct
     1441 gtttcccgcc gctgatgcca ggttggccat tcgaccaggc tgtttcggtt gataccggcg
     1501 tgatttttac ggccgccgac gtcgatccgg gtcattgctg acatcgagcg gcaacgagta
     1561 aacccttaac ttcgtcatta acttcgtaag tatcattaac ttcgaaaagg cactcagtaa
     1621 tatgcttaat ttcgaaggtt gtttctgaac aaactaagcc ctttaaaaa
//
LOCUS       DADTFG010000319         1659 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.321, whole genome shotgun sequence.
ACCESSION   DADTFG010000319 DADTFG010000000
VERSION     DADTFG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.321"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..1534)
                     /locus_tag="J1138_004730"
     CDS             complement(<1..1534)
                     /locus_tag="J1138_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HBA8456395.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKSSLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNKYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEP"
BASE COUNT          482 a          387 c          315 g          475 t
ORIGIN      
        1 tcggttcaag atatccaaat gcatctctgg aaaacttaag cataaaattt ttcatatcag
       61 aaattgcttc ttgtaaaaaa aggggatcat cgccatgatt atccagcagg atggcaattt
      121 tttcgcacat attctctttc ctcagctctt ccctgggttg cagccttttc atggtatgat
      181 gttcaatttc tttcaggagt aatgaggcat ttatctgacg tctggtactg ccttgcgtca
      241 gccagtggat ggcctgggaa aaacactgtt gtgcaacggt gaaataaaca tcattcttga
      301 tgctactatt cagtgcctgc ttttgattat gcctatgatg gattagcgtc acaaccaact
      361 tttgcaatgg tgaaccatcc tgattgacaa tatcttgggc atgtgatttc tcgcagtatt
      421 tattgagtaa tgcaacaaaa ctgttaacca gttctttcaa tatatcaggt atctcttttt
      481 gtgttataaa ttctccagga atacccaaca gttctagata acagtaataa catgcttcat
      541 gaaaagtttt ctgaaatatc tcctcaacct tcaaacgggc tgcaagagtg aagtcagggt
      601 caaacatata ttttccgata tacaagcaga gatcagacag cccttgcata agtaccatcg
      661 gttcttttgc cagttcagct aatcttagtt tatacccaaa atcagctggg gaatctcccg
      721 gaaacagaca actgagtccg ttaaaatcca ttaatctgcc atccattgca atattagaag
      781 gagaagttgt caatctcagg aaacgtgttc gacaaaatgc catttgccag gcctcacgac
      841 gtgccagttc acataacccc tcaatgcaat aaatccgggg atcagttcgt gcttcagcat
      901 caatttcttc cggtggtaca ggtagatatc ctggcagctt gtggatcaca gatctaaccc
      961 gacaggcatc gtgaattaac agactggaat actccggttt tacttggaag taaggtgccc
     1021 gttcaaaatg cgccgggcga acaacaggct cacgtaccaa cagggctctt cgtgatttcc
     1081 taccggagcg ctcaaatgcc ttttcagtat agagatctgt aagtaaaacc gcccgaaccc
     1141 gcacagcact ataaggtaat atttgagcca gtacttctcc ccacaaagcc tcatatattg
     1201 catgaacagc gccgagtgca ccattagaat gacgttcgtc agtaccttcg ccaaccaacg
     1261 gattacttcc tattccttta acctggtagt taccatcaaa cccacaacgt gcaccacctc
     1321 cattacgctg aataccactg ccaccatagc gctctgcatg gaacactttg gcagaagctg
     1381 tactgccagg atcgataata taactgcagg cattttgcag ctcgtctgga tttctgacat
     1441 cctctgcaca ccagattaat gaggacttaa tctgcctggc agtgaaaggc accaggtttt
     1501 cattcagttc agaaagtgaa cactgatgac tcatctggca gtattctccg tttcaatttt
     1561 cataaaagag tgttcataaa agagcaagta agtgtcctga cagcttcttc cttattacag
     1621 taaaactcta ttaattacac ataaattaaa aaattattt
//
LOCUS       DADTFG010000320         1651 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.322, whole genome shotgun sequence.
ACCESSION   DADTFG010000320 DADTFG010000000
VERSION     DADTFG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.322"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            370..1332
                     /locus_tag="J1138_004731"
     CDS             370..1332
                     /locus_tag="J1138_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456396.1"
                     /translation="MEGKLIFCSDAILRFQSDYDETSAVPLLSIQNTIANTDPFFLLR
                     FFHHTVLIEEGTSLASIFLAIEPWKALLAAYLDRDVGAYIDEVRKPSGPTTWDIEWIG
                     IDRRSSVYRAYKRQDMEEGEDFSTYFNRERFPTDEFDIESSCDASGFIKGDKERWSIS
                     GDVHEIKNLPVILYSKQALMTSAKDGLLKKNVSGVKSSKHGCFVYGDTSFSFSEVMEA
                     IFISGLFFYAPIDAASSLDELKVSLAGLEEELAEVPKVDSNGNETDKEPTIVVAEGAF
                     DSVAAHMETEAEEWQSIKNLCQREGELPIRIGGIKMAEPPEFHF"
     gene            1393..>1651
                     /locus_tag="J1138_004732"
     CDS             1393..>1651
                     /locus_tag="J1138_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBA8456397.1"
                     /translation="MPHKKVALQLIEETLKELESPKGSLLSAIQRLQRTSDIINDDDK
                     KIWCAIQLGDTKYTKPITELLKFVIEAENTKNKSFQENLDKR"
BASE COUNT          519 a          286 c          386 g          460 t
ORIGIN      
        1 caaagtagaa gatactgaac atggaataaa actcactcat tgaaaatgac aatgatattt
       61 atgtcaataa ttatagccat tatctgttat cggagtctgt aagtcgttgc gataaagata
      121 ggttgaaaaa tacttatatt cgcttggttt cagaactcac acaaatgagt gaagaaacta
      181 taaaatcaca gatgttataa tctgagttat cagtttcaga tattgtgatg ttatccataa
      241 ggtcaatgct tttgggttga atatatatcc agagctatca acgaacatgg gtcaggttaa
      301 agtcgaatgc ggtaagtggc agtgttctgg taaaatgcgt tgtctgatag cgtcaggagg
      361 cgatgagcga tggaaggtaa attaattttt tgttccgatg caatactgcg gtttcagtcc
      421 gattacgatg aaacctcggc ggttccattg ctatccatcc agaacacgat agccaatacc
      481 gatcccttct ttcttcttcg cttcttccac catactgttc tgattgaaga aggaacatcg
      541 ctggcaagta tctttctggc aatagaaccg tggaaggcac tgttagcagc ttatctggac
      601 agagatgttg gtgcttatat agatgaggta agaaaaccgt ccgggccaac gacatgggat
      661 attgaatgga ttgggattga ccgtcgtagc tcggtttatc gcgcttacaa gcgtcaggat
      721 atggaagaag gggaagattt ttcgacctat tttaatcgtg agcggttccc gactgatgag
      781 tttgacatag aaagtagctg cgatgcttca ggtttcatta aaggtgataa agaacgctgg
      841 agtatcagcg gtgatgttca cgaaattaaa aatcttcctg taatcctcta tagcaaacag
      901 gcgttgatga cgtcggcaaa ggacggcctg ttgaagaaga atgtatcagg ggtgaagagt
      961 tccaagcatg gctgctttgt ttatggcgat acctcgtttt ctttcagtga ggtaatggaa
     1021 gctatcttca tcagtgggct gttcttctat gcgccaatag acgccgccag tagtcttgat
     1081 gaactgaaag tgagtttggc tgggctggaa gaagaactgg ccgaagtacc aaaagtggat
     1141 tcgaatggca atgaaacaga taaagaacct actatagtcg tagccgaagg tgcttttgat
     1201 tcggtcgctg ctcacatgga aacagaagct gaggagtggc aatctattaa gaacctgtgc
     1261 caacgagaag gtgagctgcc catacgtatt ggtggtatta aaatggctga gccaccagag
     1321 tttcattttt agtttaaaag caacaatcta ccacttaatg attacgaagc tataagttga
     1381 aggatgattc acatgccaca caagaaagta gcattacagc tcattgaaga aacacttaaa
     1441 gaactcgagt caccaaaagg ttctttgtta tcggctatac aaagactaca gcggacttcg
     1501 gatatcatca atgatgatga taaaaagata tggtgtgcta ttcaattagg tgatactaaa
     1561 tatacaaagc caataactga attgcttaag tttgttattg aggctgaaaa tacaaaaaat
     1621 aaatctttcc aagaaaattt agacaaacga a
//
LOCUS       DADTFG010000321         1642 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.323, whole genome shotgun sequence.
ACCESSION   DADTFG010000321 DADTFG010000000
VERSION     DADTFG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.323"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..740)
                     /locus_tag="J1138_004733"
     CDS             complement(<1..740)
                     /locus_tag="J1138_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019453416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBA8456398.1"
                     /translation="MSIAFTPWPAEFASRYRERGYWIDKPLTEILDRQANNDASAIID
                     VQGSLTYRELQQRSDRLAAALLRRGVKSGDTALVQLGNVAEFYIVFFALLKIGVAPVN
                     ALFSHQRSELNAYAEQIKPALLIADRRHALFADDQFLNAFRDAHPSLRAVALRSQAEG
                     ELALGAWLEEASDGFVAQPSAADRVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEI
                     CRFDAQTRYLCALPVAHNYPMSSPGVLGV"
     gene            complement(748..>1642)
                     /locus_tag="J1138_004734"
     CDS             complement(748..>1642)
                     /locus_tag="J1138_004734"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HBA8456399.1"
                     /translation="RWFDRAAFMAGVRPSANGPELAGVTELPPQQTFMNMVSDAVAAM
                     KAGELDKVVLSRLLEIETRQPVDRHALMARVIAQNPHGFHFHVPLEQGALLGASPELL
                     LRQDGGRFYSNPLAGSARREADPQRDREVGERLMASAKDRHEHRIVTESMRDVLAGRC
                     RYLNVPHTPELLTTTTLWHLSTPIDGEAASPDENALSLACLLHPTPALCGMPTAEAHA
                     LIGKLEPFDRGLFGGIVGWCDDEGNGEWVVTIRCGTVDGNRVRLFAGAGIVEDSSPES
                     EWHETGTKLSTILRAFGMNQG"
BASE COUNT          288 a          533 c          545 g          276 t
ORIGIN      
        1 acgcccagca cgcccggcga actcatcggg tagttgtgcg ccaccggcag cgcgcacaga
       61 tagcgggtct gcgcgtcgaa acggcagatc tccacgctgc ggcgaatgct gtagtagtaa
      121 tcgttgtggg tgcgcgggat cagcttcggc gtgccggtgc tgccgccgga aagctggaag
      181 aacgccaccc gatcggccgc ggaaggctgc gcgacaaaac cgtcgctggc ttcttccagc
      241 caggcgccca gcgccagctc gccctcggcc tggctgcgca gcgccacggc acgcagcgaa
      301 ggatgcgcat cgcggaaggc gttcaggaat tgatcgtcgg caaacagcgc gtgccgacga
      361 tcggcgatca gcagcgccgg tttgatctgt tcggcatagg cgttcagctc gctgcgctga
      421 tggctgaaca gtgcgttgac cggcgccacg ccaattttca gcagcgcgaa gaacacgata
      481 taaaattccg ccacgttgcc cagctgtacc agcgcggtgt cgccggattt cacgccgcgg
      541 cgcagcaaag cggccgccag gcgatcggag cgctgctgca gctcgcggta ggtcaggctg
      601 ccctggacgt cgataatcgc cgaagcgtcg ttattggcct ggcggtcgag gatctcggtc
      661 agcggcttgt caatccagta gccgcgctcg cgatagcggc tggcgaactc cgccggccag
      721 ggggtaaagg caatgctcat cttgtcgtta tccttgattc ataccaaagg cacgcagaat
      781 ggtgctgagt ttggtacctg tttcatgcca ttcagattcg ggtgaagagt cttccacgat
      841 gccggcgccg gcgaacagcc gcacccggtt gccgtcaacg gtgccgcagc ggatagtcac
      901 aacccactcg ccgttgcctt cgtcgtcgca ccagccgacg atgccgccga acaggccgcg
      961 atcgaacggc tccagcttgc cgatcagggc gtgcgcctcg gccgtcggca tgccgcacag
     1021 cgccggcgtc gggtgcaata ggcaggccag cgacagggcg ttttcgtccg gagacgccgc
     1081 ctcgccgtcg atcggcgtgg agagatgcca cagcgtggtg gtggtcagca gttcaggggt
     1141 gtgcggcacg ttcaggtagc ggcagcggcc ggccagcacg tcacgcatac tttcggtgac
     1201 gatgcggtgc tcgtggcgat ctttcgccga ggccatcagc cgctccccca cttcgcgatc
     1261 gcgctgcgga tcggcctcgc gccgggcgga accggccagc gggttggaat agaaacggcc
     1321 gccgtcctgg cgcagcagca gctctgggct ggcgcccaac agtgcgccct gctccagcgg
     1381 cacatggaag tggaaaccgt gcgggttctg ggcgatcacg cgcgccatca gcgcatggcg
     1441 atccaccggc tggcgcgttt cgatctccag caggcgcgac aagaccactt tatccagctc
     1501 accggccttc atcgcggcaa cggcgtcgct gaccatgttc atgaacgtct gctgcggcgg
     1561 cagctccgtt accccggcca gttcagggcc attcgccgaa ggccgaacgc cggccataaa
     1621 ggcggcgcgg tcgaaccagc gg
//
LOCUS       DADTFG010000322         1590 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.324, whole genome shotgun sequence.
ACCESSION   DADTFG010000322 DADTFG010000000
VERSION     DADTFG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.324"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            34..951
                     /locus_tag="J1138_004735"
     CDS             34..951
                     /locus_tag="J1138_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456400.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKINVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            1036..>1590
                     /locus_tag="J1138_004736"
     CDS             1036..>1590
                     /locus_tag="J1138_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBA8456401.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILYRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTDAV"
BASE COUNT          450 a          362 c          374 g          404 t
ORIGIN      
        1 tatgtttaat ttttaagcaa ccggagaaag aggatggttc agaaaatttt gtcggataag
       61 gttatgaatg aaagaacaaa tgcgtattac tcttattatc ttggagagcg taatatctcc
      121 gtactgcccc ttaatgttta tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac
      181 agagaaaatc tgaacatcac actttcagat tttgaacttg aacaaataat ttctggtatg
      241 cgtctgaagg ccctggcctt cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt
      301 gaacgcgcat gcttattttc ctggtatctg ttgatgcagt ttatccagaa taacagggca
      361 aaaataagcg cagacttgct tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa
      421 gggaatgcct tcccgtcgga ctcatcgaca cagttcagac aaatactcag agttctcgat
      481 attctttctg ataagaattt gcgggatgag tggataatcc agaccaaaga cagatggatg
      541 cgggcattta aatcaaagag tcctttcagt tacctcctcc cggagaacga acatgaatgc
      601 atatggacat ggaattattt gaagggaaaa aatattgcac tggaaaaact ggccagtttt
      661 cctggttctg ctgatattta ccacgcaatt cacctttcat ttgacatatg ggttacctgt
      721 ccactcacct cacctgacga tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg
      781 cagcgcaagt acaaaaaaat gcaggaagat aaaatcaatg tacagttttt tcttgacgca
      841 gaaaccaggg cgcagctcaa agaattatcg agggtcagaa gactcagtac cggcgaaatg
      901 ctgcatgacc tgattgttga agagtataaa aggtaccgac attccagata atttcagaca
      961 tatattatct ccgtgagtcc cccaccacct ttccggtgtg cgggggtttt gtcttttttc
     1021 accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa accgctctcc
     1081 tccctgcctt actcactttc ccgtcacatc ctggaacatc tgcgcaaact caccagtcac
     1141 gaacccgtga ttggcattat gggtaaaagc ggggccggta aatcctcact ctgtaatgca
     1201 ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcacccg ggaagtgcag
     1261 cgcttccgtc tgagtggcca cggtcacagc atggttatca ctgacctgcc cggggtgggc
     1321 gagagccggg acagggatgc agagtatgaa gccctgtacc gtgacattct gcctgaactt
     1381 gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga tgagtatttc
     1441 tggcgacaca tcctgtaccg gggacatcag caggtgctgt ttgtggtgac gcaggccgac
     1501 aaaacggagc cctgccatga atgggatatg gccggcattc agccttctcc cgcacaggca
     1561 cagaacattc gtgagaaaac agatgcggta
//
LOCUS       DADTFG010000323         1585 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.325, whole genome shotgun sequence.
ACCESSION   DADTFG010000323 DADTFG010000000
VERSION     DADTFG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.325"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            34..651
                     /locus_tag="J1138_004737"
     CDS             34..651
                     /locus_tag="J1138_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456402.1"
                     /translation="MIMTSLSPDTVRRIEDAAAALVAAGTPNPTNEQVRTHLGGGSLS
                     HISPVMRAFRARLREQRAAQAPALPAELAQLLSGQLALLWQAAVKQADADTLAAREQA
                     EADIALADQERDAAQATAARLDSELAVLREVVAERDRLLQEVRVLRDEALPLREQVAR
                     LTATGEHLAAQLKDTRAELKGAREETRTLQGELLTLARGHGKVKQ"
     gene            957..1454
                     /locus_tag="J1138_004738"
     CDS             957..1454
                     /locus_tag="J1138_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011154565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456403.1"
                     /translation="MEAEPIESGGPLGTFLQKVRGLEGTITTREVTLDVIPGDGHELY
                     GWLKATADSLLPASGQGWAPGSIPLLVSTEILPQYRTGTVAGASDKATHVTEQTNRQP
                     MTHVGTITECYRPREVRLRATKTMWISECGLRFRKATGAAVGSGVWSANRLDLSSIRE
                     IKSGE"
BASE COUNT          310 a          498 c          522 g          255 t
ORIGIN      
        1 gtacgatgac ggcatccccc gacagaccgg agcctgatca tgacctcact ctccccggac
       61 accgtccgcc gtattgaaga cgccgccgcg gccctcgtcg ccgccggcac cccgaacccg
      121 accaacgagc aggtgcgcac gcatctcggc ggcggctcgc tgtcccacat ttccccggtg
      181 atgcgcgcgt tccgcgcccg cctacgcgaa cagcgcgcgg cgcaagcgcc ggcgctgccg
      241 gctgagctgg cacagctgct gagcggccag ctggccctgc tgtggcaggc ggccgtgaaa
      301 caggccgacg ccgacaccct ggccgcccgt gaacaggccg aggcggatat tgcgctggcc
      361 gaccaggagc gcgatgccgc gcaggcgact gcagcccggc tcgacagcga gctggcggtg
      421 ctgcgcgaag tggtggccga gcgcgatcgc ctgctgcagg aggtgcgcgt gctgcgggat
      481 gaagcgctgc cgctgcgcga gcaggtcgcc cggctgaccg ccaccggcga gcacctggcg
      541 gcgcagctga aggacacccg ggccgagctg aaaggcgccc gcgaggaaac ccgcaccctg
      601 cagggcgagc tgctgacgct ggcacgcggc cacggaaagg tgaagcaatg agagcggaaa
      661 tccaggcccg tgtgaaggct tttcgccagg cgctggcgct cgccgccggc gaatgcgccg
      721 gtgagccgat accgtaccgc tggcgtgcct atctggcaga ctttccgcgc ggctgttgtg
      781 agctggccag ccagacactg gtcgagtatc tgaaagagta tgacgacacc ctgctcccct
      841 atgttatcgc cctgcagtgg actgaaaatg atgacctgca cggtcacgtc tttgccgccc
      901 tggacgggga gtacatcgac ctgacgctgg atcagtttga cgggtatgac gcctggattg
      961 aggccgagcc gatcgagtcc ggcgggccgc tgggcacctt cctgcagaag gttcgcggtc
     1021 tggaaggcac gatcaccacg cgtgaagtga cccttgatgt cattccgggt gacggccatg
     1081 agctttatgg ctggctgaaa gccacggcgg acagtctact gcctgcgtca ggacagggct
     1141 gggcacctgg cagcatacct ctgctggtat ccacggagat cctgcctcag taccgcacag
     1201 gaacggtcgc cggtgcgtca gataaggcca ctcacgtcac tgaacagaca aaccggcaac
     1261 caatgacgca cgtggggacc attacagaat gctaccggcc ccgcgaggtc aggctgcggg
     1321 caacaaaaac catgtggatc agtgaatgcg gcctgcggtt cagaaaggcc accggcgccg
     1381 cagtcggcag tggcgtctgg agtgcaaacc gtcttgattt aagcagcatc cgggaaatta
     1441 agtctggtga ataaaagccg gtgcgcaggc agatgctaag gtttttacgg ccagtgcagc
     1501 cggtaaataa agcagggtaa tcagtacagt aaaagtgccg ttataccgtg tcgcccggac
     1561 atttttatgt aaccgtttgg ccgct
//
LOCUS       DADTFG010000324         1583 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.326, whole genome shotgun sequence.
ACCESSION   DADTFG010000324 DADTFG010000000
VERSION     DADTFG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.326"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>1583
                     /gene="tssI"
                     /locus_tag="J1138_004739"
     CDS             <1..>1583
                     /gene="tssI"
                     /locus_tag="J1138_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBA8456404.1"
                     /translation="LNGQLEQYEIFDYPGRYKDEQHGKDFTLYRIESLRSDAEKATGQ
                     SNSPKLWPGTRFTLTGHPQKMLNREWQVIQSILSGYQPQALHGSQGRGTTLGNQLEVI
                     PADRTWRPRVQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVKFHWDRYHGMTEESSC
                     WVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPLVMGRTYHEDNRSPGDLPGTK
                     TQMTIRSKTYKGSGFNELRFEDATSNEQVYIHAQKNMDTEVLNDRTTDVKHDHTETIG
                     NDQKITVVKGQTVQVGTRKEGGHDQSITVANDRRITVRNDQTLKVTNDRTVSVSHDDG
                     LYVRNDRRVTVKGKQEHKTTGNHVSLVEGKHSLVVKGDLARKVSGALGIKVDGEIVQE
                     SSSRISLKVGGSFVVIHSGDVDIVGPKINLNSGGSPGTPVPTLQPTVLKTPGGEKTGD
                     GSDSGEENEDPGGSGLAGSGGGDRGDDEDEPEKYTLQFHFTDDDGIPYSEIRYIAFFE
                     DGAQIRGETDKDGYTEVFS"
BASE COUNT          414 a          342 c          562 g          265 t
ORIGIN      
        1 cctgaacgga cagcttgaac agtatgagat atttgattat ccgggaaggt acaaggatga
       61 acagcacggc aaggatttca cgctgtaccg gatagagagc ctgcgcagtg atgcggaaaa
      121 ggccacaggg cagagtaatt cgccgaagct gtggccggga acgcggttta cgctgacggg
      181 gcatccgcag aagatgctga accgtgaatg gcaggtgata cagagtattc tgtcagggta
      241 ccagccgcag gcgcttcatg gcagccaggg gagaggaacc acgctgggaa atcagttaga
      301 ggtgataccg gcggaccgga cctggcgtcc acgggtgcag agcaaaccga aggtggacgg
      361 gccacagagc gccattgtca ccggtcccgc aggagaggaa atcttctgcg acgaacatgg
      421 tcgggtacgg gtgaagtttc actgggaccg ttatcacggg atgacggagg agagttcgtg
      481 ctgggtgcgt gtgtcgcagg cgtgggcggg gccgggattt ggtaatctgg cgataccgcg
      541 ggtgggccag gaggtgattg ttgattttct gaacggggac ccggaccagc cgctggtcat
      601 gggccggacg taccatgagg acaaccgttc gccgggggac ctgccgggaa cgaagacgca
      661 gatgaccatc cggtcaaaaa cctataaagg aagcggattt aacgagctgc ggtttgaaga
      721 cgccaccagt aatgagcagg tgtatatcca tgcgcagaag aacatggaca cggaggtgct
      781 caacgaccgg acaacggatg tgaaacatga ccatacggaa accatcggta acgaccagaa
      841 gataacggtg gtgaaggggc agacggttca ggtcgggacc agaaaagaag ggggccacga
      901 ccagagtatc acggtggcga atgaccggcg cataacggta cgcaacgacc agacgctgaa
      961 ggtgacgaac gaccggacgg tgagcgtgag tcatgatgac ggcctgtacg tcagaaatga
     1021 ccgtagggtg acggtgaaag ggaagcagga gcataagacg acggggaacc atgtcagcct
     1081 ggtggaggga aagcacagcc tggtggtgaa gggggacctg gcgaggaagg tgagtggcgc
     1141 gctggggata aaggtggacg gggaaatcgt gcaggagagc agcagccgga taagcctgaa
     1201 agtgggtggt tcgtttgtgg tcatccattc aggggacgtg gacatcgtcg ggccgaaaat
     1261 caatctcaat agcggcggta gtccgggaac gccggtgccg acgctgcaac cgacggtgct
     1321 gaagacaccg gggggtgaga agactggtga tggcagtgac tccggagaag aaaatgaaga
     1381 ccctggaggg agcggcttgg ccggaagtgg tggtggtgat cgaggtgacg atgaggatga
     1441 acctgaaaaa tatactctcc agttccattt tacagacgat gacgggatac cgtattcaga
     1501 aatacgctac attgccttct ttgaggacgg tgcgcagatt cgcggtgaaa cggacaagga
     1561 cgggtataca gaagttttct cta
//
LOCUS       DADTFG010000325         1499 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.327, whole genome shotgun sequence.
ACCESSION   DADTFG010000325 DADTFG010000000
VERSION     DADTFG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.327"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(343..1407)
                     /gene="phoH"
                     /locus_tag="J1138_004740"
     CDS             complement(343..1407)
                     /gene="phoH"
                     /locus_tag="J1138_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBA8456405.1"
                     /translation="MVTSCTGHVLDNQRATTRGDFSSGSHLVTLYFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVRSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
BASE COUNT          386 a          392 c          348 g          373 t
ORIGIN      
        1 atgtattcgg tgttaatcag tcagtcaaat tatggtccat tattgatgga acctcatacg
       61 caaaattggc accagacctc ctgtacaggt tgaatggctg aaaaaagata ggcagatgct
      121 cgctttttgg gcttctgaaa gaaaaactct ccgcactgac ggtattacac tggtctggtg
      181 ctatgacgac aaatgcccgg taagagtatc taaacctgaa ttcatttcag cttatcaaca
      241 cgctcaaagt cgccccctga acgttaacct gaagtgaaaa tctttgctgt taatgaaata
      301 aaacaaagcc cggttcgccc gggctctgca ccgataatac acttaactgt atgcatgtag
      361 cgtacgttgg caaagtgccg aacgtacgca atcctcttta ccgaatcgga cgatcccgac
      421 catttcatcc tcttcgaaac gttccagcgc gtcacttaat ccggagcgca cgccgcgagg
      481 caaatcgcat tgcgtgatat caccgttgac gataaccgtc acgttctccc cgaggcgggt
      541 taaaaacatt ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa tgacaactgc
      601 attttcaaag gtacgtccac gcatataggc gaacggcgca atttccacct tccctatttc
      661 cggtcgcagg cagtactgca taaaggaagc ccctaagcga cggaccagca cgtcgtagac
      721 cgggcgaaaa tagggagcaa acttttctgc gatatcacca ggtaagaaac caagatcttc
      781 atcggcttgc agaactggac gggtaacgat aatcctgtcg acatccttat gtatcagggc
      841 ctctgccgct tttgctgcgc tgatccaggt ttttccgcac ccggcttcgc ccgtggcaaa
      901 tatcagctgc ttactctcaa tagccttcag atagtgcaat tgcgcttcat ttcgcgcgag
      961 gatgggcgag gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc cgctcatctg
     1021 cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag
     1081 cacacgtttt gcctcgcgac gagctttgat cactgctttt tgtcttccca tggagagcac
     1141 cttgagttgt ttgtattcat cacacgcgcc gttggcagcg cgattatgcg cacgaacatc
     1201 agagggttgg cttccttgta agccatagtt tgcttttgga taaaatgccg aaaaacggct
     1261 acgcgcaccg tttacggcgt cggtaacaca tgaaaagaaa ggatgaggtt gaaaatacaa
     1321 agtgacgaga tgactaccgg aggagaaatc tccgcgagtg gtggcgcgct gattatctaa
     1381 aacatgtcca gtacaggacg ttaccatccg cgatctccat agtgactgac tatcactgcc
     1441 gggaacttcc gctgctactt aataagtaca acagatttcg cattcattgc aacaatata
//
LOCUS       DADTFG010000326         1439 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.328, whole genome shotgun sequence.
ACCESSION   DADTFG010000326 DADTFG010000000
VERSION     DADTFG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.328"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            29..334
                     /locus_tag="J1138_004741"
     CDS             29..334
                     /locus_tag="J1138_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456406.1"
                     /translation="MVLKGWALIGDAMNHAMFPGIVLAWIIGMPLGIGAFIVGLSCAI
                     ATGYLKDNSIIKKDTIIGIVFSGIFVLTINRHKSADYRYHYYITVYSAWYRLPFLPH"
     gene            360..566
                     /locus_tag="J1138_004742"
     CDS             360..566
                     /locus_tag="J1138_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456407.1"
                     /translation="MLLIAPGSIAVLITHRFHKALLIAVTISITISGVWLLYFLDRAP
                     APTIVLLFAVAFLFALIIINFRKQ"
BASE COUNT          455 a          295 c          218 g          471 t
ORIGIN      
        1 gttcccctgt gcattattat cagttttcct ggtattaaaa ggctgggcgc taataggaga
       61 tgcaatgaac cacgcaatgt ttcctggcat agttctagcc tggattatag gtatgccgct
      121 gggaattggt gcatttattg taggactgtc ttgtgcaatt gcaactggct atctgaaaga
      181 taatagcatc ataaagaaag ataccattat aggtatcgtt ttttcaggaa tttttgtctt
      241 gactatcaac aggcacaagt cagcggatta cagatatcat tattacatta cggtttactc
      301 tgcatggtat cgcctaccat ttttgccaca ttaaaagcag tcgaaattat tctgtctata
      361 tactattaat agctccaggg tctattgctg tcctcatcac gcatcgcttt cataaggcat
      421 tacttatcgc cgtaacgata tcaataacaa tatcaggtgt atggttatta tattttctcg
      481 atagagcccc agcgccgaca atcgtcctct tatttgctgt agcatttctt ttcgcgctta
      541 taataatcaa ttttcggaaa caataatctc aacagtagaa taaagaggca aatcaatcat
      601 tacatatata agatggtgct aatattgcac cgcctttaaa ttgaacttta tcctttcacc
      661 cttacacaat ttaaggaatg cagatgacta ttttatcagc acccagcctt tttcctggta
      721 atcgaaaact tgaaaatcat taactctaag aaattctgcc aaatcgttga aattacttta
      781 aattagactc gaacaaatca atccctgatg ggacttttac ttatcaattt aatcgtgtag
      841 atatcgacct ccatctgccc gcctcagatt cgtacctggt ggaagtcttt tttcgttacc
      901 tctttccccc tttagttcag tataaaatca tcaaactaat gctgttcaca ctatttacct
      961 ccagcattct atataaataa tagaaataaa aaccccaata catagtgcat tggcatgacc
     1021 aaacatcaat gccattatac tcgcctctgt taaatactcg ctagttaata tgataggcac
     1081 gcatgatata acaatccata tgctaataca taaatacatc agtatttttc tcatagtcag
     1141 tccctgtgat taacaatcaa ggcatagtta agcatatttc acattcaaac gtaaacatta
     1201 ttaataacat tatgtcaatc gaactgacat cacgattcaa gccctcttca gactgaaggt
     1261 tagagcagcg tagaacattt caaaacaata cactaaaaaa atacttgaac aaaaaactta
     1321 attctttttt attgtgaact ttagtaattt tgtcatgcaa ctctttatta catttaggcc
     1381 tatcttattt catggcaacg cagatacagg accatttctt gtgctatgga taagtaata
//
LOCUS       DADTFG010000327         1437 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.329, whole genome shotgun sequence.
ACCESSION   DADTFG010000327 DADTFG010000000
VERSION     DADTFG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.329"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            238..663
                     /locus_tag="J1138_004743"
     CDS             238..663
                     /locus_tag="J1138_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBA8456408.1"
                     /translation="MQHAHSVDLQVCEADRLSFLPYLFDADFLRAEMLVYATADRYLS
                     GYNGGFWHFIRLPNGGGYMAPDIDQVKLSNADNWFEKTISGDAAGIILTSLVINRRCW
                     FHHDRGNAGMVQMYIQREEQLKAYIDTHPERADIWGALD"
     gene            725..901
                     /locus_tag="J1138_004744"
     CDS             725..901
                     /locus_tag="J1138_004744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456409.1"
                     /translation="MKLDDETQKHLRRYQAIINEDRLQYGLSPLTLPQVVAAVFEYLA
                     DQPCIFLRGVFIRQ"
BASE COUNT          305 a          399 c          420 g          313 t
ORIGIN      
        1 cagctgcagc gtagtgacgg cctgtgccgt ccccaaacgc tagtccggct cggttgtgtt
       61 caagcgccgc ggagctcgcc gtcggcaccc gttcgcaccc gttacgggca ttcagccacg
      121 caacgggctt gcgctgcggc tttgacggcg cccttgacca ctgccctcaa ggccggcctt
      181 tcgaatgggt acggcaccgg ccggtgtcgc taaccctgaa actgtggagg tagtcccatg
      241 caacatgctc attccgtcga tctgcaggtg tgtgaagccg atcgtttgtc attcctgccg
      301 tacttgttcg acgcggattt cctgcgcgct gaaatgctgg tttacgccac cgccgatcgc
      361 tacttgtcgg gttacaacgg cgggttctgg catttcatcc gcctgccgaa tggcggcggg
      421 tatatggcac cggatattga tcaggtgaag ttgagcaatg ccgataactg gttcgaaaaa
      481 accatcagtg gcgatgccgc cggcataatt ttgacgtcgc tggtcattaa ccgccgctgc
      541 tggttccatc acgatcgcgg caatgccggc atggtgcaaa tgtatatcca gcgggaagag
      601 cagttgaaag cctacatcga tacgcacccg gagcgggctg atatctgggg cgccctggac
      661 tgactgaagg cggcggggct gtttgccccg ctttgaacaa caacacagaa ccccgaggtt
      721 gcccatgaaa ctggatgatg aaacccaaaa acatctgcgc cgatatcagg cgatcatcaa
      781 tgaagaccgt ctgcaatatg gattatcccc gctgacgttg ccgcaagtgg tcgccgccgt
      841 ttttgaatat ctggcagatc agccgtgcat cttcttgcgg ggagtgttta ttcgtcagta
      901 aagctggatt tgccgcgtag ccttacatct gtggggctac ctggcagcgc ggtaaaaacc
      961 ctgtcacctg cggtgaccgg cagaagagag cggcggcgat gccgccgcct ccagctgccg
     1021 acgaaggctg gatacccatg ctgcagtagt aaggcatcgg acgtggtatg aaaaaatgat
     1081 gaagggtatc cattatgaaa aggaaaactt cttctggttt taaaaggaat aactgcttgt
     1141 ttataagaac agggtttgga aagtagcggt tggccaccct tgtattgcca taccacatca
     1201 cattaccggg atatttgggc aaggttggga gagagaaaaa acacgagggc ttggggcaat
     1261 ggccggtcga atgaccgccc agcagaccta ctcaaccaag acgcccgtat gcgggcgtca
     1321 ggtttcgccg aacccgcaag cggtttcggc ctgtcggtta gggtctcccc gccaacggca
     1381 acacccgcgc ttgctgccgg cgtcgccggc gccgctttca ctgctcgtcg tttggac
//
LOCUS       DADTFG010000328         1430 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.330, whole genome shotgun sequence.
ACCESSION   DADTFG010000328 DADTFG010000000
VERSION     DADTFG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.330"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..516
                     /locus_tag="J1138_004745"
     CDS             <1..516
                     /locus_tag="J1138_004745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456410.1"
                     /translation="KIIKDEAAQLKELMDEQLEIPSFFSCVKPAILMEVWMVLCPLVS
                     FFLADQRDSELLIATGMSAFLGFLISVSIVNGRSLYLSLPVQLRESSQVLELLSKKVK
                     SYAMVFLSLVLILSVLAANSALGSMAYIIPLIFFTVMMVFIFNMDIGRYRLSAFTAAL
                     ELIKSRKQGGE"
     gene            519..728
                     /locus_tag="J1138_004746"
     CDS             519..728
                     /locus_tag="J1138_004746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456411.1"
                     /translation="MAKISAKTHTTTLRLDDGTFGRLQRMVAENPLYSPSHVMRGALL
                     ALSRLTDSEREACILAVAKGGVDEL"
     gene            718..>1430
                     /locus_tag="J1138_004747"
     CDS             718..>1430
                     /locus_tag="J1138_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HBA8456412.1"
                     /translation="MSFNAKDMTQGGQIAFMRLRMFTQIGNLIFYCLFIAFFLVAICT
                     LLWRVSIQNLTNGLIYWWVWMVEGWPSISPSETLYHLDYYGKTLTYTAKQVLLDSYVS
                     YCGQLIWREGIFACIVAAVACVTGFVVTVWILGRQGKQQSEDEITGGRTLTDNPAVVA
                     NMLKRDGMASDIIIDGLPLLKNSEVKNFCAMGSVGTGKSRLYRKLMDYARKRGDMVIV
                     YDKSCDFVKDYYDPQHDKIL"
BASE COUNT          340 a          326 c          380 g          384 t
ORIGIN      
        1 aagataataa aagatgaggc tgcacagttg aaagaactga tggatgaaca acttgagata
       61 ccatcattct tttcttgtgt taaacctgca atactaatgg aagtctggat ggttttatgc
      121 ccactggtta gtttcttttt ggctgatcaa agggattcgg aattactcat tgcaacagga
      181 atgagtgcgt ttcttggttt tttaatatca gtaagcattg tcaatggacg gagcttgtat
      241 ctctcactac cagttcaatt gagagagagt tcgcaggtgt tggagttgtt aagtaaaaaa
      301 gtaaaaagct acgcgatggt atttttatca ctggtgctga tattatcagt tttggcggcg
      361 aatagtgctt taggttctat ggcatatatc ataccgctta ttttcttcac agtaatgatg
      421 gtgttcattt ttaatatgga tattggccgc tatcgtcttt cagcatttac cgccgctctg
      481 gagcttatca aatcccgcaa acagggaggt gagtaacggt ggctaagatc tctgccaaaa
      541 cgcataccac caccctgcga ctcgatgacg gtacgtttgg gcgcctgcag cgtatggtgg
      601 ctgagaaccc cctctacagc ccctcacatg tgatgcgcgg tgccctgctg gctctcagtc
      661 gattaaccga cagtgaacgt gaagcctgca ttctggccgt agcaaaggga ggtgtggatg
      721 agctttaacg ccaaggacat gacgcagggc gggcagatcg cctttatgcg cctgcgcatg
      781 ttcacgcaga tcggcaacct gattttctac tgcctgttta tcgccttctt cctggtggcg
      841 atttgcacgc tgttgtggcg cgtgtccatc cagaacctca cgaacggcct gatttattgg
      901 tgggtctgga tggtggaagg ctggccgagc atatcgccgt cagagaccct ctaccacctg
      961 gattattacg gcaaaacgct gacctacacc gccaaacagg tgctgctcga cagttacgtc
     1021 tcctactgcg gtcagctgat ttggcgtgaa gggatctttg cctgcatcgt ggcggcggtc
     1081 gcgtgtgtga cgggttttgt ggtcaccgtg tggatacttg gccgtcaggg caagcagcag
     1141 agcgaggatg aaataaccgg cgggcgaaca ctcaccgata accctgcggt agtggccaac
     1201 atgctcaaac gtgatggtat ggcgtccgat atcattattg atggcctacc gctgctaaaa
     1261 aacagcgagg tcaaaaactt ctgcgccatg ggcagcgtcg gtacgggtaa gtcacggctg
     1321 tatcgcaagc tgatggacta tgctcgcaaa cgtggtgaca tggttattgt ctacgacaag
     1381 tcttgtgatt ttgtcaaaga ctactacgat ccgcagcacg ataaaatcct
//
LOCUS       DADTFG010000329         1424 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.331, whole genome shotgun sequence.
ACCESSION   DADTFG010000329 DADTFG010000000
VERSION     DADTFG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.331"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..1110
                     /locus_tag="J1138_004748"
     CDS             <1..1110
                     /locus_tag="J1138_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456413.1"
                     /translation="NGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDP
                     AEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLS
                     TGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLAL
                     QKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSG
                     VAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKIL
                     AMIQEQVTWLAGSFLLTGHEIENLNENLAHVIDQNALEIIFAAGIQQRAATNMLIKPL
                     VVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            1186..>1424
                     /locus_tag="J1138_004749"
     CDS             1186..>1424
                     /locus_tag="J1138_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456414.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVT"
BASE COUNT          337 a          366 c          389 g          332 t
ORIGIN      
        1 aacggacagt atcgttacat gggaaataat ggccccatgc aactggaagt cccccgtgat
       61 cagtatgccg gcgctgttga aaccatgagg aataagatcc gcgaaggtaa agtacccggt
      121 gtaaccgatc ccgcagaagc gtcccggctg attcgtcggg gacatctgac ttatactcag
      181 gcccgtaata tcacccggtt cgggaccatc gaatcggtca cttatgatat tgccgagggt
      241 tcggttgtca gtctggcggc cggagggatc agttttgccc tgacggcatc ggtcttctgg
      301 ctcagcaccg gcgatcgcga tgctgccctg cagacagctg ctgtccaggc aggaaaaacc
      361 ttcacccgca cactggctgt ctacgtcaca acccagcaac tccaccggct cagtgttgtt
      421 cagggtatgc tgaagcacat tgatttttcg actgccagcc cgactgtccg actggctctt
      481 cagaagggga ccggtgcagg aaatatcagt gccctgaaca aagtaatgaa gggtacgctg
      541 gtgacatctc tggcactggt agctgtcaca accggccctg acatgatcaa aatgttgcgg
      601 ggacggatct ccggtgcgca gttcatcagg aatcttgccg tggcatcttc cggtgtggca
      661 ggtggagctg tcgggtcagt ggcgggcggg atactgttca gtccactggg accatttggt
      721 gcactgacag ggcgtgtggt tggcggtgtt ctggggggaa tgattgcctc cgctgtatca
      781 ggaaaaattg ccggagcgct ggttgaagaa gatcgcgtca aaattctggc aatgattcag
      841 gagcaggtga catggcttgc cggcagtttc ctgctgaccg gacatgagat tgaaaatctg
      901 aacgagaatc tggcccatgt tatcgatcag aatgctctgg agatcatttt cgccgccggt
      961 atacaacaac gggccgcgac caatatgtta atcaaaccac tggtggtcag tatcatcagg
     1021 caacgccccg tcatggaata tgatgcatcc catctcggca atatggttaa ccgactggaa
     1081 gaagcattac ccccggagtt accggcataa cggctattct gcgcctttta cgctttatca
     1141 cctgatttgt cagtgtcatc gtcatgactc agagaggtaa ataccatgat tcacctgttc
     1201 aaaacctgca tgattaccgc cttcattctg gggttaacgt ggtctgcccc actccgggca
     1261 caggatcaac gttacatcag tatacgcaat acagatacga tatggctccc gggaaatatt
     1321 tgtgcttacc agttccggct ggataatggc ggaaacgatg aaggatttgg ccccctcacc
     1381 atcactctgc aactcaaaga caaatatggt cagacgctgg tgac
//
LOCUS       DADTFG010000330         1373 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.332, whole genome shotgun sequence.
ACCESSION   DADTFG010000330 DADTFG010000000
VERSION     DADTFG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.332"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..349)
                     /locus_tag="J1138_004750"
     CDS             complement(<1..349)
                     /locus_tag="J1138_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456415.1"
                     /translation="MVDIVEADKTDIYFIQESVYGKIGLPAFGNTIGPSAQQVVKKVF
                     AVVKERDKTHAKQRLLLEYNGNKLWMNAIDGSEAILPTEFSKRYELSLFNTTNFGEDP
                     FPDVNLYNNMKSSF"
     gene            complement(578..1342)
                     /locus_tag="J1138_004751"
                     /pseudo
     CDS             complement(578..1342)
                     /locus_tag="J1138_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          396 a          307 c          227 g          443 t
ORIGIN      
        1 aaaaacttga tttcatatta ttatataaat taacatcagg aaaaggatct tctccaaaat
       61 tagtagtatt gaataatgaa agttcgtatc gcttagaaaa ttctgttggt aggatagctt
      121 cactgccatc aatagcattc atccagagtt tgttaccatt atattccaac aataatctct
      181 gttttgcatg tgtcttatct cgttctttaa cgacagcgaa taccttttta acaacttgct
      241 gtgcggatgg tcctattgta ttaccaaaag ctggcaaccc gattttgcca taaacactct
      301 cctgaatgaa ataaatatcc gttttatcgg cctcgacaat atctaccatt tatccctcca
      361 aacaagacct gactccagga caagagcata gtgtgtagta aacacttcat gctcttgccc
      421 tgaccattga aataacaact ttcgtttgtt tactgtacgc tgtctaaata gctgcgtgga
      481 ataaagttgc actttttatc atcccttgca gagcaagaat acgaaagccc gcccccgcaa
      541 ggactgacgc gaggtagtct ctgtccgcag ctgcttttca catcttacat cttgcccctg
      601 ccttgagtat cttatctttc gttaaaatat taatagcgat cttccgtatc cctgaaaata
      661 attctgctgc atttcctctt cttattttgc agtcgtcttt attcattacc acgtccagat
      721 gccagtgcag cttattctcc acgtaccagt gatttcggtt cgctgtggcg aacttctctg
      781 cggttaaatc agcagaaatg ctataatatc tgtccgtcat ttctggctct ttcttttgtt
      841 ctgttattat tgaacgaaag gagactgcca tgcataattt cttcagctct ttccattcaa
      901 acgtgaaatc aataagttca tcagggacat cgcaaataat atgaagacgg attttaatga
      961 cataaatcgc tcccttgcgg cgactcttgt cataagagtg ccgaagcgtt tttccatcaa
     1021 ttgcaatgac atctttatca tctgaggaat ggcagtcacg catccagtta ataaagcact
     1081 catgaaattt tgcaggactg atacaggata caactctggc aatgatatcg tgaacaggaa
     1141 taccattttc aaaatcacca tatcacttca aaaaatcgag atgtgtttcc ccaaatcctc
     1201 tatatcttcc cagcattatg caccagaaat aacggcgcaa atagttaaca gatagaatat
     1261 ctggcaattt atgttccact ttccaggctt gcctgtaatc gggaataata gaaatatgtt
     1321 ccatcaattt tttaagttcc attttgtccc ccttaattag gcaatgagta ttt
//
LOCUS       DADTFG010000331         1371 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.333, whole genome shotgun sequence.
ACCESSION   DADTFG010000331 DADTFG010000000
VERSION     DADTFG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.333"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>1371
                     /locus_tag="J1138_004752"
     rRNA            <1..>1371
                     /locus_tag="J1138_004752"
                     /product="16S ribosomal RNA"
BASE COUNT          343 a          320 c          431 g          277 t
ORIGIN      
        1 aataccgcat aacgtcgcaa gaccaaagag ggggacctta gggcctcttg ccatcggatg
       61 tgcccagatg ggattagcta gtaggtgggg taaaggctca cctaggcgac gatccctagc
      121 tggtctgaga ggatgaccag ccacactgga actgagacac ggtccagact cctacgggag
      181 gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc cgcgtgtatg
      241 aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa agttaatacc
      301 tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca gcagccgcgg
      361 taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac gcaggcggtt
      421 tgttaagtca gatgtgaaat ccccgggctc aacctgggaa ctgcatctga tactggcaag
      481 cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg tagagatctg
      541 gaggaatacc ggtggcgaag gcggccccct ggacgaagac tgacgctcag gtgcgaaagc
      601 gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat gtcgacttgg
      661 aggttgtgcc cttgaggcgt ggcttccgga gctaacgcgt taagtcgacc gcctggggag
      721 tacggccgca aggttaaaac tcaaatgaat tgacgggggc ccgcacaagc ggtggagcat
      781 gtggtttaat tcgatgcaac gcgaagaacc ttacctggtc ttgacatcca cggaagtttt
      841 cagagatgag aatgtgcctt cgggaaccgt gagacaggtg ctgcatggct gtcgtcagct
      901 cgtgttgtga aatgttgggt taagtcccgc aacgagcgca acccttatcc tttgttgcca
      961 gcggtccggc cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg
     1021 acgtcaagtc atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca
     1081 aagagaagcg acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg
     1141 agtctgcaac tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg
     1201 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa
     1261 agaagtaggt agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt
     1321 gaagtcgtaa caaggtaacc gtaggggaac ctgcggttgg atcacctcct t
//
LOCUS       DADTFG010000332         1370 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.334, whole genome shotgun sequence.
ACCESSION   DADTFG010000332 DADTFG010000000
VERSION     DADTFG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.334"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            363..1187
                     /gene="gadX"
                     /locus_tag="J1138_004753"
     CDS             363..1187
                     /gene="gadX"
                     /locus_tag="J1138_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBA8456416.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPSLLFHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          405 a          252 c          275 g          438 t
ORIGIN      
        1 tcacgttgta aaaaccgaat gcccaacctt taaaaacagg tgggtattcg gctacttatt
       61 aattgcaata atatattacc tgtctattca tcgtgttgat aagatatatg ttatatgaat
      121 gttatggagc atagcgatac tgcttaatat tacgatgatg aattatttgc taactattaa
      181 ttcctcttcg ttcttaagtc ccctttttat ataataaatc cagtcatcca gcccgagtat
      241 catcgctcat tatcatcctg ttttcccgct tcgtttaaat ttatttatca atcaatttga
      301 cttaagaggg cggcgtgcta cattaataaa cagtaatatg tttatgtaat attaagtcaa
      361 ctatgcaacc actacatggt aattgtctaa ttgcgtacgc aagacataaa tatattctca
      421 ccatggttaa tggtgaatat cgctatttta atggcggtga tctggttttt gcggatgcaa
      481 gccaaattca agtggataag tgtgttgaga atttcgtatt agtttcaaga gacacgcttt
      541 cattatttct cccgatgctc aaggaggagg cattaaaact tcatgcacat aaaaaagttc
      601 cttcattact ctttcatcac tgtaccagag atattcctgt ttttcaggaa gttgcgcaac
      661 tatcgcagaa taagaatctt cgctatgcag aaatgctacg taaaagggca ttaatctttg
      721 cgttgttatc tgtttttctt gaggatacgc agtttatacc gttacttctg aacgtgttgc
      781 aaccgaatat gcgaacacga gtttgtacgg tcatcaataa taatatcgcc catgagtgga
      841 cattagcccg aattgctagc gaattgttga tgagtccaag cctgttaaag aaaaaattgc
      901 gcgaagaagg tacgtcatat tcacagttgc ttactgagtg taggatgcag cgtgctttac
      961 aacttattgt tatatatggg gtttcaatta agcgagttgc agtatcctgt ggatatcaca
     1021 gcgtgtcgta tttcatttac gtttttcgaa attattatgg gatgacgccc acagagtatc
     1081 aggagcgatc ggcgcaggaa ttaccgaact gtggctcggc ggcaagtatg gctgcgcaag
     1141 ggatttttta cggcactgac cgttctgcgg aaggaataag attatagagt tttactcaga
     1201 cataaaaaaa acccggcata ggggaccggg aagaggatag tctgccgtct ccagactaat
     1261 aaaccgttat aacactccct gttggcacgg gaaactttgt gctctcagta agttaaatat
     1321 aacttttact ggaaataaga tcagccattt ttttataaac ataagctata
//
LOCUS       DADTFG010000333         1303 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.335, whole genome shotgun sequence.
ACCESSION   DADTFG010000333 DADTFG010000000
VERSION     DADTFG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.335"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            93..179
                     /gene="azuC"
                     /locus_tag="J1138_004754"
     CDS             93..179
                     /gene="azuC"
                     /locus_tag="J1138_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBA8456417.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            293..544
                     /locus_tag="J1138_004755"
     CDS             293..544
                     /locus_tag="J1138_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBA8456418.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(623..1126)
                     /locus_tag="J1138_004756"
     CDS             complement(623..1126)
                     /locus_tag="J1138_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBA8456419.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
BASE COUNT          370 a          288 c          297 g          348 t
ORIGIN      
        1 ttacatcaca taaagattga tttttgtgat ggttgtcata ttataaacgt aaagacaata
       61 acacctgtat aacaaatagt cggagtaccg cgatgaaact gcgcaagatc ctgaaaagta
      121 tgttcaataa ctattgcaag acgttcaaag acgtaccgcc aggcaatatg ttccgataac
      181 aaaaaacctg ctccggcagg ttttttgtgt tctgatgacg gtggatgtga ctatcgctga
      241 agatgtgaag ccgctactat aacggctttc aaaatgctat caggagttta cgatgtccca
      301 gccgctgaat gccgatcagg aactggtttc tgatgtggtt gcttgccagt tggttatcaa
      361 acaaattctg gatgttctcg atgtgatcgc gcccgttgaa gttcgcgaaa aaatgtccag
      421 ccagctgaag aatatcgact ttactaacca tcccgccgcc gcagacccgg tcaccatgcg
      481 ggcaatccaa aaagccatcg cgttgattga actcaagttt accccacagg gcgaatccca
      541 ttaaaaaacc gctgcttcaa aaaaatgagg cagcggtttc tcaaaaactt catcggcgca
      601 atgcattagc gccgatgtat gatcaatgca gctgatgcga cacgacattc agaacatgtt
      661 ggtcggtctg cacaggacac ataccagcaa gtttcgcact gcgcacttca tcaagaatgg
      721 tttgcagtaa caggccatca tgctgctgtt ctttttccag atcgcgcagg aaattgacgg
      781 ttgaatcatc attcagttct ttcgcttcat cggctaactg cgccagcgtg cttgaacgtt
      841 gctcgtattc ttccatcgtt ttttggaaca gttcttccag agagttcagt ttttcaccgg
      901 gaacatcaat ggctttaacg atgggggtag cgccgacact cttcataaag ttaaacatgc
      961 gcatcatttg ggtcacatta ctctgtgcct gggcgcgaag gaaagtggcg gtgccgttca
     1021 gactctgttc agaacaccag ttactcaggt gaaggtagag attggatgcg taaaactcgc
     1081 ggttcatttg agagttgagt ttgagaagca ttccagcggt tgccatacct aatatcctta
     1141 tatccagaag tggagaggtg caagataaat caaaacacca taattactgt ttgcagaata
     1201 attcctttct gccggtccga aatgtgatcg tggagtcaat tctgacgatg atctgaaata
     1261 agaataacgt ctgaacgggt ataagaaaat gcttaaatca tca
//
LOCUS       DADTFG010000334         1297 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.336, whole genome shotgun sequence.
ACCESSION   DADTFG010000334 DADTFG010000000
VERSION     DADTFG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.336"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            211..729
                     /locus_tag="J1138_004757"
     CDS             211..729
                     /locus_tag="J1138_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBA8456420.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPSGQRAHKPFIFTVALNKAVPLLYNALASGEMLPKVELHWWRTS
                     VEGKQEHYFTTRLTDATIVDMNLHMPHCQDPAQREFTQLLAVSLAYRKVEWEHIKSGT
                     SGADDWRAPLEA"
     gene            950..>1297
                     /locus_tag="J1138_004758"
     CDS             950..>1297
                     /locus_tag="J1138_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBA8456421.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIWQGVIPQRYVTGVVAGFGMQENNGWQMRYHLCIEPPLWRC
                     GLRRNFRIFQQQDI"
BASE COUNT          313 a          301 c          361 g          322 t
ORIGIN      
        1 agcttatgtg accgtgttct gaaattgact aaaaatatat ttaaacacca taaaagagtt
       61 tttcggtagg tgctaattac gtgtgcctgt aactcgaata aagaaaaggg tgtggttcct
      121 gatgtgagcc gcatcacact ctaacctgtc ggggattaac gacgggttgt tcgtaaaaca
      181 gcagttgata atttcacaag gagttcataa atgccaaccc catgttacat ttcaattaca
      241 ggtcagaccc aggggaacat cactgccggt gctttcaccg cagattctgt cggcaatatc
      301 tacgtgcagg gacacgaaga tgaaatgctg gtgcaggagt ttctgcataa cgttactgtt
      361 ccaaccgatc cgcagtctgg tcagccttcc ggccagcgtg ctcacaaacc gttcattttc
      421 accgtggcgc tgaacaaagc cgttccgctg ctgtacaacg cgctggcctc cggtgaaatg
      481 ctgccgaaag tggagctgca ctggtggcgt acctctgttg aaggcaagca ggagcactac
      541 ttcaccacac gtcttactga cgcgacgatt gtggacatga acctgcatat gccgcactgc
      601 caggaccctg cacagcgtga gtttacgcaa ttgctcgcag tatcactggc ctaccgtaag
      661 gttgagtggg agcacatcaa atccggtact tctggtgccg atgactggcg tgcaccgctg
      721 gaagcataaa tctgagtcaa caacatcctg cctccgtgca ggatgttgtt tttgtacgtg
      781 taaaaaatga gtgtcagtaa ggagttacgc tttcatgagg gagatggtgg atgccggaaa
      841 cccgggcggt agcgtacctg aaaatgtaaa gatgagtgta acgctgcact ggcacccttt
      901 gctgcatggt gggaatcatt ttgtccgtga atgaatcagg gaggtcatta tgtccttaaa
      961 aggtcttcgc tttacgctgg aggttgacgg ccaggagccg gacacctttg cagtggtgaa
     1021 tttcaggtta atccagaacc agtcttatcc gtttgtgatg agtgtggatg tcgccagcga
     1081 ttcttttatg cagacggcgg agatgctgct ggagaagaag gcaacgctga ccatctggca
     1141 gggcgtcatc ccgcagcgtt atgtcacagg cgtggtggca gggtttggta tgcaggagaa
     1201 caacggctgg cagatgcgtt atcacctgtg tattgagccg ccgttatggc ggtgcgggct
     1261 gcggcggaac ttccgtatct tccagcagca ggatatc
//
LOCUS       DADTFG010000335         1266 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.337, whole genome shotgun sequence.
ACCESSION   DADTFG010000335 DADTFG010000000
VERSION     DADTFG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.337"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..177
                     /locus_tag="J1138_004759"
     CDS             <1..177
                     /locus_tag="J1138_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HBA8456422.1"
                     /translation="AGCKEEAKTTKWYRDHPDELKVVYDKCQKTGDASENCKNANEAH
                     WQIQQLNAPEVDFN"
     gene            189..1259
                     /locus_tag="J1138_004760"
     CDS             189..1259
                     /locus_tag="J1138_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBA8456423.1"
                     /translation="MAGIFSSVEKNLIGGITNATSGLMMSYSSMMMGLAATSVTIYIM
                     WRGYQTLAGKLSTPMEDTMWDIMRMAIILSFVANVSGYLDGVIDAINGLKEGFSGGES
                     IWKMLDNLWKSARVLGKKLHDMDNSTYVKDEGWTAQFYVWLGIFVLMTITAFVSMIAE
                     VMILLLSITAPIFIFCLMYGFLRPMFNNWLQNIFAAILTILFSALSLRIVINFLTSRM
                     AEATAQADKVNIVELGAQVCLAGICAAILVYLSAKLAASLAGASATVSMQGLAAMGIG
                     AAAYGSGKLAGGAVKETGRSARDNIQGWKDANAGKPGKPGGELGYNAAKARKYAIEQM
                     IARNEARRLAQSRDSAVKKFNS"
BASE COUNT          361 a          257 c          330 g          318 t
ORIGIN      
        1 gctggatgca aggaggaagc taaaacaaca aagtggtatc gtgaccaccc agatgaatta
       61 aaagttgttt atgataaatg tcagaaaaca ggggatgcat cagagaattg taaaaacgcc
      121 aatgaagctc actggcaaat acagcagcta aacgccccag aggtagattt taattagagg
      181 atttactcat ggctggtatt ttttcttcag tggagaaaaa tttaattggt gggataacca
      241 atgctaccag cggtctaatg atgtcttatt cttctatgat gatgggactg gcagcaacgt
      301 cagtcaccat ctacatcatg tggagaggct atcagacact ggctggtaag ctctccacgc
      361 cgatggaaga cacgatgtgg gacatcatgc ggatggcgat catcctgtcg tttgtggcaa
      421 acgtcagtgg ttatctcgat ggtgttatag acgcaataaa cggattaaag gaggggtttt
      481 ctggcggaga aagcatctgg aaaatgctgg ataatttatg gaaaagtgca agagtattag
      541 ggaaaaaact acatgacatg gataattcca cttatgttaa ggatgaaggc tggacagctc
      601 agttttatgt ctggcttggc atatttgttt taatgaccat tacagccttt gtttcgatga
      661 tcgctgaagt aatgattcta ttactgagca ttacggcccc catcttcata ttttgcctta
      721 tgtatggttt cctgcgtccg atgttcaata actggctgca aaacatattt gcagccatat
      781 taacaatact attttcagct ttatctctac gtattgttat aaatttttta acatcgagaa
      841 tggcagaggc caccgcacaa gcggataaag taaatatcgt agaattagga gcacaagtct
      901 gtcttgcggg tatctgcgcg gcaatactgg tttatctctc cgcaaaactg gccgcatccc
      961 tggccggggc aagtgctacc gtatccatgc aggggctggc tgccatgggc atcggtgctg
     1021 ccgcctatgg ttccgggaaa ctggccgggg gagcagtaaa agaaaccgga cgttccgccc
     1081 gggacaacat ccagggctgg aaggatgcga atgcaggtaa gcccggcaag cccggaggtg
     1141 agttaggcta taacgctgct aaagcgcgta aatacgccat cgagcagatg atagcccgga
     1201 acgaagcgcg gaggctggca cagtcgagag attccgcggt taagaagttc aacagctaag
     1261 ccagac
//
LOCUS       DADTFG010000336         1256 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.338, whole genome shotgun sequence.
ACCESSION   DADTFG010000336 DADTFG010000000
VERSION     DADTFG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.338"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(67..1011)
                     /gene="leuO"
                     /locus_tag="J1138_004761"
     CDS             complement(67..1011)
                     /gene="leuO"
                     /locus_tag="J1138_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBA8456424.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          382 a          285 c          252 g          337 t
ORIGIN      
        1 aattctacat tcaataaaaa atgcagaata aaccagacat tgatgtctga cctattctgc
       61 attcagttag cgtttgcaaa ttgagactaa ttgctcttcc atccactgat ggcctttatc
      121 gcgcccggca gcttcatgcc aggagagata acaggttctg ctgttttgtt ttaacggcag
      181 cggtaatacc tgtaactcta aggattcagc gaactcttca gccaaccaac gcggcgcaat
      241 agcgaccaga tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata
      301 cgcgatactg gcttgcttat ctaccgtgtc ataccaggtt tgactaaatg atgcgaaacg
      361 atcgagcgaa accgcagcgt gttgttcgtt ataaacatca tgcttcagta acgggccttt
      421 aattgttgga tgatttttgc tggctaccaa caccatttca tctttaaata atggcacact
      481 ggtaaattca gggcgatgga agtcttcata actaatcaca aactccgttt cctgataacg
      541 cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc
      601 aatctgctca atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac
      661 acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt
      721 ttgtactagt tgcaatgcct gacgaactga gccaaaaagt tgaaatgcgc gagcagtcgg
      781 ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc
      841 aacagcgtta ctgaccgcag gttgcgacat tcccagagca tgagcggcac gggtgatgtt
      901 ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg
      961 tggcttgctt aactccgccg tctctggatg atctgtttgt acctctggca tacttaactc
     1021 cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc ctcgagaata
     1081 tgatttatca tgcatataaa ataagaaaaa gcaaaatgag taaaattagg aaaacataaa
     1141 gatacaggtt ctgataaaaa cagaatccac tgccgagcaa agagtcattt actacagcat
     1201 aataatccat aatgtttttg ttgcgaaaac aatctaatca taactaccac gaatac
//
LOCUS       DADTFG010000337         1254 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.339, whole genome shotgun sequence.
ACCESSION   DADTFG010000337 DADTFG010000000
VERSION     DADTFG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.339"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(115..1224)
                     /locus_tag="J1138_004762"
     CDS             complement(115..1224)
                     /locus_tag="J1138_004762"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBA8456425.1"
                     /translation="MFSIPPRGAAAVETWMYQVAQRTNFPNRIVCVKNPGYSNYTFVN
                     DNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLYS
                     QIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGID
                     LETYQSTFQPITRSELNISAEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVV
                     IGDYTEMSKSDKGAYQRNVREIANRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQ
                     EPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANP
                     ELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          393 a          252 c          212 g          397 t
ORIGIN      
        1 tccgtggaaa cgtagaacaa attgaaccaa atttattagg cgtttacgcc ggcttattat
       61 tagcgatgaa aaacaagtaa aaaataaaaa ggctgcataa tgcggccttt ttatttattc
      121 aaaccaatta tgaataacct cttcaaatct ttgagttacg ccatcccaac tgtagtgatc
      181 aaacacaaaa tcctgacctt gctttgcaac tgctgttaat tcaggattcg ctaacgtttt
      241 taaaatatcg ctgcttatag agtctgcagt cataggttct tttaaatgga atcccgtagt
      301 attttctttg acaaattccg tcatcccccc acgcgtactc actaaaacag gcttaccagc
      361 ccccatagct tcaattgcca ccatgcaaaa tggttcctgg aactgggaag gaatgacgac
      421 taaatctgcc agagggtaat aacaatgcat cttttcaggc ggtatgcttc ctaacataat
      481 gcaacgatcc ttcaaacggt ttgcaatttc cctgacattg cgttgatatg cacctttgtc
      541 acttttactc atttctgtat aatcaccaat cacaactaat tttaaatttt tgtgagcagc
      601 tgccaatttt tcgaatgatt gcatcaacag tagtatgcct ttgtcaggga cgatacgccc
      661 agcataaaaa ataattttct cttcagcgga gatattaagt tcgcttctag tgatcggctg
      721 aaaagtagat tgataggttt ctaaatcaat tccatttggt acaatctcaa tatctgcatt
      781 tgctaaataa gattggtaat actttttcaa gtataaacta ggcactatca ttttaacatt
      841 ttgctcaagc aattttggtt caaatgcatt atgcatatgt ataaccactc ttgcctgagg
      901 tgctcgctta cgaatttgag aatatagctt catgctatta tgaactacaa taacactttc
      961 tttcgttata ggaaaatcat gtgcaatatt caatattctc tgtgaatatg gaagaggatc
     1021 aagtcttgtc catttttgaa ataaccgctt atatatgcgg ctgaatccaa ccctatgaat
     1081 actacaatta tcattaacaa aggtataatt tgagtatcct ggattcttga cacaaacaat
     1141 acgatttggg aaattggtac gctgagcaac ctggtacatc cacgtttcaa cagcggccgc
     1201 tccgcgagga ggaattgaaa aaataggagt aactgtaaat attattttat caac
//
LOCUS       DADTFG010000338         1246 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.340, whole genome shotgun sequence.
ACCESSION   DADTFG010000338 DADTFG010000000
VERSION     DADTFG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.340"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            278..649
                     /locus_tag="J1138_004763"
     CDS             278..649
                     /locus_tag="J1138_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBA8456426.1"
                     /translation="MNTQNVNTATKESSKRWAGKYPDAESIMIRQLAFMEELHGVYLS
                     PEELAQSLYEIFNTMTDRVVFNGVDNSPSDKPKIAYAVAQHWIKAQRLAGAYFGETGV
                     VAWEHARYRLHLQLAINHSYR"
     gene            708..>1246
                     /locus_tag="J1138_004764"
     CDS             708..>1246
                     /locus_tag="J1138_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA8456427.1"
                     /translation="MRLTSRFGRYNAIRKDRPLTNDELMNVVPSVFSEDKHDSRSDRY
                     TYIPTITLLDKLREEGFQPFYACQTRVRDESKRGHTKHMLRLRRENMIVSGNKEVPEI
                     VLLNSHDGSSSYQMIPGMFRYVCANGMVFGQSFGDVRVPHKGDVVGQVIEGAYEVLGI
                     FDRVDNDLDTMKCISLSER"
BASE COUNT          301 a          330 c          331 g          284 t
ORIGIN      
        1 aacgctaagg tcgccccggc ttgtcgccgt gtcggccgtg ttcctcctcg ctgcgtttcc
       61 ggtgagccat cggacggccg ttccccggcg gagggctgtt cgtagcaccg tcgcgtcacc
      121 ccgcaagggg tgaaatgcac ttcaccgaaa actttttcca ggtaaaaact tttcgcctcg
      181 ccccttgcgt gtctccttac gccgttgctc cgtcgccctt tgccgccagg gaactcaccg
      241 aacgatggaa taaccaccag agaggagcaa gagcaggatg aacacgcaaa acgtcaacac
      301 cgccaccaaa gaatcttcca aacgatgggc gggaaaatac cccgacgcgg aaagcatcat
      361 gatccgtcaa ctggcattta tggaggaatt acacggcgtg tatttgtccc ctgaagaact
      421 ggcgcaatcg ctgtatgaaa tttttaacac gatgacggat cgggtggtgt ttaacggcgt
      481 cgataactcg ccgtccgata aaccgaaaat tgcgtatgcc gtcgcgcaac attggattaa
      541 ggcgcagcgt ttggccggtg cgtattttgg cgagaccggc gtcgttgcct gggagcatgc
      601 gcgttatcgg ctgcaccttc agctggctat caatcactca taccgttaat tcatcgccga
      661 tctagaaacc atttgcccgg cattactcaa agagaaagga gaatatcatg cgtctgacat
      721 cccgttttgg ccgttataat gccattcgta aagaccgccc gttaaccaac gacgaattaa
      781 tgaacgtggt gccgagcgtg ttttcagaag ataaacatga ttcgcgcagt gaccgctata
      841 cctacatccc caccattacg ctgctggata aattacgcga ggaaggtttt cagccgtttt
      901 atgcctgcca gacccgcgtt cgggatgaaa gcaaacgcgg gcataccaaa cacatgctgc
      961 ggctgcggcg ggaaaatatg attgtctccg gcaataagga agtgcccgaa atcgtattat
     1021 tgaacagcca cgacgggagc agcagttacc aaatgatccc cggcatgttc cgctatgttt
     1081 gtgccaacgg aatggtattt ggtcaatcgt tcggtgatgt tcgggtgccg cataaaggcg
     1141 atgtggtcgg gcaggtgatt gagggtgcct atgaagtgct cggcatattt gaccgcgtcg
     1201 ataatgacct cgataccatg aaatgcattt cactgtcaga gcgcga
//
LOCUS       DADTFG010000339         1241 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.341, whole genome shotgun sequence.
ACCESSION   DADTFG010000339 DADTFG010000000
VERSION     DADTFG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.341"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            235..729
                     /locus_tag="J1138_004765"
     CDS             235..729
                     /locus_tag="J1138_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBA8456428.1"
                     /translation="MLFKLCKTLAMACVALIIAQSSALSFTLEVAGKIDNVTDPVKKS
                     YLFTDKQLLAMPVHSITTSTSWTPQRKFEGISVADILERVGAKGETLTFYALNDYYID
                     VPLSDVKKYNMILAYKMDGEMLKLRNFGPLFLVYPRDAAGPELNSPLYNSRFIWQVDR
                     IVIK"
     gene            726..>1241
                     /locus_tag="J1138_004766"
     CDS             726..>1241
                     /locus_tag="J1138_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015379378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HBA8456429.1"
                     /translation="MKLTTFKNGSRLAIPAIFAALFLVASTVTLYYYSATLSKKGLYA
                     IAGTQENYSWSIAKFSIKLAEFDTFVEQQSHAPQVDNDNLRLRFEILYSRFYVLETVS
                     ESTQPLYAEPGYPEVVKQMRLQMDRIDKLLDNPKIDFNQVFAAMRAIKPYAIEMANLT
                     DHAEVKQRTAAY"
BASE COUNT          271 a          373 c          344 g          253 t
ORIGIN      
        1 ctccccctcg ctcgccatgc tgacggaagg cctgactcag ctggcggggc tgttggaaga
       61 tagccggatg gctgacgaat cgggggacga gtgaggcaca tgacgcgatc gggccgacgg
      121 gtctttttgt gctcttcacc attctcttcg cacccgcccg ctttttctgc cattattctg
      181 cctgataaat tagcgggact gatacaaacc tccccgcgcc tctggagaac gccgatgctg
      241 ttcaaactat gcaaaaccct tgcgatggcc tgcgtggccc tgattatcgc ccagagcagc
      301 gcgctcagct ttaccctgga agtggcgggc aagatcgaca acgtgaccga tccggtcaaa
      361 aaaagctatc tgttcaccga taaacagctg ttggcgatgc cggtgcacag catcacgacc
      421 tccacctcct ggacgccgca gaggaagttt gaagggattt cggtggccga catcctggag
      481 cgcgtcggcg ccaagggcga gacgctgacc ttctacgcgc tgaacgatta ttacatcgac
      541 gtgccgttgt ccgacgtcaa gaagtacaac atgatcctgg cctacaagat ggacggggag
      601 atgctgaagc tcagaaactt cggcccgctg ttcctggttt atccgcgtga cgccgccggg
      661 cctgagctga actcgccgtt gtacaactcg cgctttatct ggcaagtcga caggatcgtg
      721 atcaaatgaa gctgacgacc tttaagaacg gcagcaggct ggcgataccg gccatcttcg
      781 ccgcgctgtt tctggtggct tcgaccgtca cgctctatta ctacagcgcc accctgtcga
      841 agaaagggct gtatgccatc gccggcacgc aggagaacta ctcttggtcg atcgccaagt
      901 tctccatcaa gctggcggaa ttcgacacgt tcgtcgagca gcagagccat gcgccgcagg
      961 tggacaacga caacctgcgg ctgcggttcg aaatcctcta ttcgcgcttt tacgtgctgg
     1021 aaaccgtctc cgaatccacg caaccgctgt acgccgagcc gggctacccg gaagtggtca
     1081 agcagatgcg cctgcagatg gatcgcatcg ataaactgct ggataacccg aaaatcgact
     1141 tcaatcaggt tttcgcggcc atgagggcca ttaagcctta cgccatcgaa atggccaacc
     1201 tgaccgacca cgccgaggtg aagcagcgca ccgccgccta t
//
LOCUS       DADTFG010000340         1229 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.342, whole genome shotgun sequence.
ACCESSION   DADTFG010000340 DADTFG010000000
VERSION     DADTFG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.342"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(54..1136)
                     /gene="ompC"
                     /locus_tag="J1138_004767"
     CDS             complement(54..1136)
                     /gene="ompC"
                     /locus_tag="J1138_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBA8456430.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTDGQ
                     VAYGKSKFNASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEA
                     VAQYQFDFGLRPSVAYLQSKGKDLGVHGDRDLVKYVDVGATYYFNKNMSTFVDYKINL
                     IDDSKFTKTAGIDTDDIVAVGLVYQF"
BASE COUNT          345 a          270 c          251 g          363 t
ORIGIN      
        1 ttatatgttg cggaggcaaa gcctcccgca acatatcttt ttcgtaagtc agattagaac
       61 tgataaacca gacctacagc gacgatgtcg tcggtatcaa taccagctgt tttggtaaac
      121 ttactatcgt caattaagtt gattttgtaa tcaacaaaag tggacatgtt tttattaaag
      181 tagtaagtag caccgacatc gacatacttg actaagtctc ggtcaccatg aacaccaagg
      241 tctttacctt ttgactgaag gtaagcaaca gatgggcgca gaccgaagtc aaactgatat
      301 tgtgctactg cttcaaagtt ttgtgctttg tttgcaatat ggttattacc aaaaacggtc
      361 atattctgag tttcagaata tgtggtagcc agatagatat tgttcgcatc atatttcagg
      421 cctgcagccc atacttccgc atttttgccg gaggcattga atttgctctt accataggcg
      481 acctgaccgt cagtgcgatc tgatttagca taggttgcac ccacgccgaa tccttcatac
      541 tcataagtag tggagaaacc gaaaccatca ccattggctt cagttacgtc agtgcggtca
      601 tttttaccct gatactgagc agcaaagttc aggccatcga ccagaccaaa gaagtcgttg
      661 ttacgataag ttgcaacacc agtagtgcga ccagtcatga acacatctgt ttgggtccag
      721 gtatcaccac cgaattctgg cagaacgtca gtccacgcac cgatgtcgta tgctacaccg
      781 tagttacggc cgtaatcgat tgagccgtaa tcaccaaatt tcaggcctgc aaatgcaaga
      841 cgggttttgt ctttggaaga accttgagat tcagcacggt tgcctttgaa ttcatattcc
      901 cactgaccga aaccagtcag ttgatcgttg atttgggttt cacctttgaa gccaagacgg
      961 gcataagtag tatcaccatc atctgcatca ttagaggaga agtagtgctt ggcattaact
     1021 ttcccgtata gatccagctt gttactgtct ttattataaa tttcagctgc ctgagcagac
     1081 atcgccatca gtactgatgc agctacagca gaaattgcca ctgttaattt tttcattgta
     1141 cgcccttttt ttgaactatt ataaaaaaat gatgtcactg cgcgataaat attcatctaa
     1201 tcaatgtgat tatttcaaga tgtaagttt
//
LOCUS       DADTFG010000341         1227 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.343, whole genome shotgun sequence.
ACCESSION   DADTFG010000341 DADTFG010000000
VERSION     DADTFG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.343"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            52..561
                     /locus_tag="J1138_004768"
     CDS             52..561
                     /locus_tag="J1138_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Tir"
                     /protein_id="HBA8456431.1"
                     /translation="MSYRNKTYVAFASEDIKFYRLMEAWKANEKIDFNFFDAHDLFIS
                     RDTSKPETIKRNLRERMKNAKQVVLLGSGNTKRKGSDGVSFLAHEIDLIVEFNLPVVI
                     ANLDGDRTVDKNFIPKPLLDSEHYTVSVSFQPKIIKYALDNYCVNYYSSSNSGSYLYP
                     TSVYTKLGL"
     gene            complement(693..>1227)
                     /locus_tag="J1138_004769"
     CDS             complement(693..>1227)
                     /locus_tag="J1138_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBA8456432.1"
                     /translation="KLFYRNNKSLAEIDWAKLQAIFHQPYEIQNDVMQDNNNTHYDFF
                     ISHAKEDKDTFVRPLVDELNRLGVIIWYDEQTLEVGDSLRRNIDLGLRKANYGIVILS
                     HNFLNKKWTQYELDSLINRAVYDDNKIILPIWHNINAQEVSKYSHYLADKMALQTSLY
                     SVKEIARELAEIAYRRR"
BASE COUNT          394 a          204 c          196 g          433 t
ORIGIN      
        1 aaggtatttt caacatgttg atactaatgt tgactgcttc atggggacta tatgagttat
       61 cgtaataaga catatgtggc ttttgcaagt gaagatataa agttttatcg tcttatggag
      121 gcatggaaag caaatgaaaa aatagacttt aacttttttg atgctcatga tctctttatt
      181 tcaagggata caagtaaacc tgaaaccata aagcgaaacc tccgcgaaag gatgaaaaat
      241 gctaaacaag tagtactgtt gggaagcggt aatactaaga gaaaaggtag cgatggagta
      301 tcgtttcttg cccatgaaat agatcttatc gtagaattta atcttcctgt tgtaatagct
      361 aatttagacg gagatagaac agtcgataag aacttcattc ctaaaccact tttagattca
      421 gaacattata cagtatctgt ctctttccaa cctaaaatta ttaaatatgc attagataat
      481 tactgtgtta actattactc aagttcaaat agtggtagtt atctataccc tacatctgtt
      541 tatactaagc taggtctgta atatttcaac gtatactaaa caatatgtgg aatgagcacg
      601 gaacactcat tccataacaa acatactatt tagatgtgct aatacaatta attttatgca
      661 tcgaagatat taagcactta tgatttattg ctttatcttc tcctgtatgc tatttcagcc
      721 aactctcttg ctatttcctt aacgctatat aaagaagttt gcagtgccat tttatccgcc
      781 aaataatggc tgtatttaga tacctcttga gcattgatat tatgccagat tggcaatata
      841 atcttattat catcatacac tgcacgatta attaaactat ctaattcgta ttgtgtccat
      901 ttcttgttta gaaagttatg agaaagtatg actatgccat aatttgcttt tcttaggcct
      961 aaatcaatat ttctccttaa gctatcgccg acttcaagtg tctgttcatc ataccaaata
     1021 attacaccaa gtctatttaa ctcgtctacc agtggtctga caaaagtatc tttatcctct
     1081 tttgcatggg atatgaaaaa atcatagtgc gtattattgt tatcttgcat gacgtcattc
     1141 tgtatttcat atggttgatg aaaaattgct tgtaattttg cccagtctat ttctgctaaa
     1201 gatttattat tgcgataaaa tagtttt
//
LOCUS       DADTFG010000342         1224 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.344, whole genome shotgun sequence.
ACCESSION   DADTFG010000342 DADTFG010000000
VERSION     DADTFG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.344"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(7..1140)
                     /locus_tag="J1138_004770"
     CDS             complement(7..1140)
                     /locus_tag="J1138_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBA8456433.1"
                     /translation="MHFYVDETGHTGPNLFDPTQPVLSYGVLSSPDDLDKVAEAGLAA
                     LRKKLKVQRLHAAELGIHRLGEVVDTLLILQKKHRMRFDVWQVVKRDHAIISFFDQVF
                     DQGMNPVVPWTAYWTPLRYPLLLNLANLFDDELAEKAWRARLEPHNERSSTLFSEVCG
                     VLLQRVDALGDPRSVEIITDALSWAMVNFDALSYNCKTNKDKLQIMPNMIGFQSVLHG
                     VCSRLGAPNRKANIIVDQQSQFNTTQRELSVFYRQIREQPWELGPGLPVMNMKNMPEK
                     PLVFQSGTQSAGLELVDIYLWIFKRFMEGKELTKPLTRLVYTNLKTARTTSVSLNSIG
                     IRFKESFGNLPEPTAEMMQKGRELIEFDEARRLKHRVPAPSQP"
BASE COUNT          284 a          332 c          334 g          274 t
ORIGIN      
        1 ggacagtcag ggctgggagg gggcagggac gcgatgtttc aggcgccgcg cttcgtcgaa
       61 ctcgatgagc tcgcgccctt tttgcatcat ttctgccgtc ggctccggta ggtttccaaa
      121 agactcctta aaccggatac cgatcgagtt cagcgaaaca ctggtcgttc gtgccgtttt
      181 gaggttggtg taaaccaggc gggtaagcgg tttggttagc tccttccctt ccataaagcg
      241 tttgaagatc cagagataaa tatcaaccag ctccagcccg gcactctggg ttccggactg
      301 gaaaaccagc ggtttctccg gcatgttctt catgttcatc accggcaggc cgggtcccag
      361 ctcccacggc tgctcgcgga tctgacgata aaagacgctc agctcccgct gggtggtgtt
      421 gaactgcgac tgctgatcca caatgatatt ggccttgcga ttgggcgccc ccaggcggga
      481 acaaaccccg tgcaggacgg actggaatcc aatcatgttg ggcatgatct gcagtttatc
      541 tttgttggtt ttgcagttgt agctcagcgc gtcaaagttg accatcgccc aggacagggc
      601 atcggtaatg atttcaaccg atcgggggtc ccccaaagca tcgactcgct gaagcaacac
      661 accacagacc tcgctgaaaa gcgtggatga acgttcatta tggggctcaa ggcgggcgcg
      721 ccacgctttt tcagccagct cgtcatcaaa aagatttgcc agattcagca gcaacgggta
      781 gcgcagcggg gtccagtagg ccgtccaggg caccactggg ttcatgccct gatcgaacac
      841 ctgatcaaag aatgaaatga tggcgtgatc gcgtttaacc acctgccaga catcaaaccg
      901 catccggtgc ttcttctgca ggataagcag cgtatcgacc acctcaccaa gacggtgtat
      961 gcccagttct gcggcatgca ggcgctgtac cttcagtttc ttacgcaaag cagcaagccc
     1021 tgcttcggca accttgtcca ggtcgtccgg cgaagaaagt acgccgtaag aaagcaccgg
     1081 ctgagtaggg tcaaaaaggt tcgggcctgt atgaccggtt tcatctacat aaaagtgcat
     1141 tcaagatccc tttatctggc agtctgaagc gaaacagtat tattcacgcg aatgtaaatc
     1201 catccagtca ttgggtgcaa gaga
//
LOCUS       DADTFG010000343         1218 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.345, whole genome shotgun sequence.
ACCESSION   DADTFG010000343 DADTFG010000000
VERSION     DADTFG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.345"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            602..987
                     /locus_tag="J1138_004771"
                     /pseudo
     CDS             602..987
                     /locus_tag="J1138_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          340 a          283 c          280 g          315 t
ORIGIN      
        1 aaaagattaa aaccgcaatg cattttttac ctgagtttta ttaaattgtc attttgagat
       61 atttatgtcc gtaaattaat ctgtaatatc gtctaagcac tatagtacag ccccaagtat
      121 tcagtgataa caaatcgtca atgctgcagc atgactgcct ttctcccact taagttgcgt
      181 cataacctgc caacgaaagc gtatctccag atgccaccag tcgatactct caagaagaat
      241 caccagttat acaagtgtaa ggtgtcaacg ccacgatttt tgaccgttac gggatgcgaa
      301 tgatttccta gactttcagg agtgaaacat gtttactgaa gaataaaaaa tccgcgccat
      361 tgagctttat ttcaaatatg gtaagaaact ggctcccgtc gtacgcgagc cgggctaccc
      421 ttagaaaaga aatctgcggc attggatccg ctcatgagaa gcaggtggtg gcgcaaaaga
      481 atccattcgt cataagcccc gttactcaga tgtgcaaaaa cagattgccg ttgaacatta
      541 tcttaaccac ggtagttgct tggcgtttac cggcagagct ctggggtatc cctacagtga
      601 tatgctcact cgctgggttg atgagcttta tcccgacaga cgacgtatat ttaccggtaa
      661 gaccaatccg gaatcgacct ttgaaccaga agtcaaacgt caggctgcca tggcgagtat
      721 cagccagtga agtcgccaga tgtattgggg tcagccgtgc ggtattgtat aaatggaaag
      781 ataaaattat cggcaatagt gcttaccaga ctatgcggaa acataacaaa ccttccctgg
      841 agacagaacg cagaacgcga tgcgttgcgg gaggaagtcg cccgactgaa cctggaaata
      901 cgccgccagc agatggagct ggatattctg aaaaaggcga aggaaatcaa aaaaaatcta
      961 tggtcgcccc cgtttttgcc gcactgattt ttaattaata gtgggcttgc ttaaatccat
     1021 ccggcgtctc tgtgggcaag gaggcccacg cctcgatgag ttccgcacct gataatccta
     1081 aaaagaccct cggcttcagt gagtaatgcc agtcagttaa gcaactgact ggctcttttt
     1141 cggggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg cctttccctt
     1201 cttgtcggta atttcact
//
LOCUS       DADTFG010000344         1214 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.346, whole genome shotgun sequence.
ACCESSION   DADTFG010000344 DADTFG010000000
VERSION     DADTFG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.346"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(147..368)
                     /locus_tag="J1138_004772"
     CDS             complement(147..368)
                     /locus_tag="J1138_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456434.1"
                     /translation="MNYQGHEKLRADVAALSNDMWELHLRLRELVSAHFWNSDVLADR
                     LAGHILRDAHDRYLEVCKAVNELDHHFRE"
     gene            complement(383..1027)
                     /locus_tag="J1138_004773"
     CDS             complement(383..1027)
                     /locus_tag="J1138_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBA8456435.1"
                     /translation="MSRYILGDSVQVMSSFPAGSIDFILTDPPYLVGFTDRSGRSIAN
                     DTNADWVLPASREMFRVLKPNSLMVSFYGWNRVDAFVGAWKAAGFRIVGHLVFTKQYA
                     SKSAFVGYQHESAYILAKGRPALPAQPLADVQSWQYTGNRHHPTEKPVSSLQPLIESF
                     TQPGAIVLDPFAGSGSSCVAAALSGRRYIGIELLPDYHATGCRRLAALRRRRAA"
BASE COUNT          243 a          377 c          389 g          205 t
ORIGIN      
        1 agcctgcgcc ctttgcggcg ccggcgacgc cggcaattgc cgcgcggcgg tatcgcggtc
       61 gggcggcgtt cgatacgctg cggcggccga cgacaaccgc ctggcgctcc gggcaggctc
      121 catagcccgc ccggtgttgc tttccgctat tccctgaaat ggtgatccag ttcgttgacc
      181 gctttgcaga cctcaaggta gcgatcgtgc gcatcacgca gaatgtggcc ggccaggcga
      241 tcggccaata catcgctgtt ccagaaatgc gcgctgacca gttcccttaa tcgcaagtgc
      301 agctcccaca tgtcgtttga cagcgccgcg acgtcggcgc gtaatttctc atggccctga
      361 tagttcatcg gtatccctcc ccttatgccg cgcgccggcg gcgcagcgca gccaggcggc
      421 gacagcccgt agcgtggtag tcaggcaaca gctcgatgcc gatatagcgg cgcccggaca
      481 aggcggcagc gacgcagctc gaaccggaac cggcgaacgg atcgagaaca atggcaccag
      541 gctgagtaaa ggactcgatc agcggttgca ggctggaaac gggtttctcg gtcgggtggt
      601 ggcggttgcc ggtatattgc cagctctgca catccgcgag aggttgtgcc ggcagcgccg
      661 gccgaccttt agccaggata taggcgctct cgtgttgata gccaacaaac gcggatttcg
      721 aagcatactg cttggtaaac accaggtggc caacgatacg gaagcctgcg gccttccagg
      781 cgccgacaaa ggcatcgaca cggttccagc catagaagct caccatcagg ctgttgggtt
      841 tgagcacgcg gaacatttcc cggctcgccg gcagcaccca atccgcgttc gtatcgttag
      901 cgatcgagcg cccggagcga tcggtaaaac caaccaagta tgggggatcg gtcaggataa
      961 agtcgatact gccggctggg aaagaagaca tgacttgcac ggagtcaccc aggatatagc
     1021 gggacataag cgttcctcgt ctgacaaggg acggcaggag ggtgccggtg cggcacccgt
     1081 tccctgcggc ccgatgtcag cgcggccagg caccgccgcg ggtcaagcgg acgtggaata
     1141 ccgccgccgc acgcggcaag gatatgccgc gaaagccgct tgaagacggc acgccatcgc
     1201 cccgcccagg ccgg
//
LOCUS       DADTFG010000345         1195 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.347, whole genome shotgun sequence.
ACCESSION   DADTFG010000345 DADTFG010000000
VERSION     DADTFG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.347"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            55..420
                     /gene="traA"
                     /locus_tag="J1138_004774"
     CDS             55..420
                     /gene="traA"
                     /locus_tag="J1138_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBA8456436.1"
                     /translation="MNASLSAQGSSVAQRQGFSLRKLFSRQRVVNFLKVILPFAVLAS
                     FFPEVALAAGGGKDMLQSGDADVKATAGSGSTMVKWIVLVEVLVGAVMYMATKNLKYL
                     SGFIVVSVLIAVGMKVAGY"
     gene            432..737
                     /gene="traL"
                     /locus_tag="J1138_004775"
     CDS             432..737
                     /gene="traL"
                     /locus_tag="J1138_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBA8456437.1"
                     /translation="MSGNDLNKYRFPKTLRTQDRWFGFCLDELIPVAITGGWFIWQQK
                     YITGIILAALVFWAIHKAKKGKGSSWLRDLAYWYLPTSLGKGVYKVVPESCFRKWIK"
     gene            748..>1195
                     /gene="traE"
                     /locus_tag="J1138_004776"
     CDS             748..>1195
                     /gene="traE"
                     /locus_tag="J1138_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBA8456438.1"
                     /translation="MEHSARLNSNRIIAIGFIVMALLIAALTVAVIIMAINNYRLQNE
                     KQVVAVPMLFKAPFGVSQNSADASYLEQMALSFVALRLNVTPETVNASHSTLLMMVKP
                     AAQNALKIVLAEEANRIRASNVNAAFFQTSVKVYPAQGRVDIRGELR"
BASE COUNT          265 a          304 c          318 g          308 t
ORIGIN      
        1 caaggatgtt tctctattgc atccaaatat ttacctcatt caaggataat ttaaatgaat
       61 gcttcattaa gcgctcaggg ctcttctgtt gcccaacgtc agggattttc acttcgtaag
      121 ctgttttctc ggcaacgcgt cgtgaatttc ctcaaggtga tcctgccgtt tgccgtactg
      181 gcttcgttct tcccggaagt ggctctcgcc gccggtggcg gtaaggacat gctgcaatcc
      241 ggcgatgccg atgttaaggc aacggcaggc tccggctcca ccatggtcaa atggatcgtc
      301 ctggtcgaag tgctggttgg tgccgtgatg tatatggcca ccaagaacct taaatacctc
      361 tccggcttca tcgtggtctc tgttctgatc gcggtgggga tgaaagtcgc cgggtattaa
      421 tcgggggcgg tatgtcggga aacgatctga ataaataccg tttcccgaaa acactgagaa
      481 cacaagaccg ctggtttggg ttttgtcttg atgaattaat ccccgtggct attactggcg
      541 gctggtttat ctggcagcag aaatatatca cggggattat tttggcggcg ctggtgttct
      601 gggccattca caaagccaaa aaggggaagg gcagtagctg gctgcgtgac cttgcctatt
      661 ggtacttacc gacctcgctc ggcaaaggcg tctacaaggt ggtccctgaa tcgtgttttc
      721 ggaaatggat aaaataggca ggcgataatg gaacactcag cacgtctcaa ctcaaaccgt
      781 attattgcca tcggctttat tgtcatggcg ctgttgattg ctgcgctgac ggtggccgtc
      841 attatcatgg ctatcaacaa ctatcgcctg cagaacgaaa aacaggtggt ggcagtcccc
      901 atgttgttca aggcgccgtt cggcgtctca caaaacagcg ctgatgcatc gtatctcgag
      961 caaatggcgc tctcctttgt cgcgttacgt ctcaatgtga cccctgagac ggtcaacgcc
     1021 tcacattcca ccctgctcat gatggtcaag ccggcggcgc aaaacgccct caagattgtc
     1081 ctggcagaag aagccaaccg tatccgagcc agcaacgtca atgcggcttt cttccagacc
     1141 agcgtgaaag tttatcccgc ccaggggcgt gttgatattc gcggtgagct gcgta
//
LOCUS       DADTFG010000346         1184 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.348, whole genome shotgun sequence.
ACCESSION   DADTFG010000346 DADTFG010000000
VERSION     DADTFG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.348"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            128..235
                     /locus_tag="J1138_004777"
     CDS             128..235
                     /locus_tag="J1138_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456439.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(288..1046)
                     /locus_tag="J1138_004778"
     CDS             complement(288..1046)
                     /locus_tag="J1138_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBA8456440.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          339 a          287 c          271 g          287 t
ORIGIN      
        1 ataaaatgca gaaacagtga ccaggtgtac aacgccaccc gacgactgta ataacaaaaa
       61 atcccttgat gcctgtccct tttgttacac tccgttatca cgcacaagag atatgcagga
      121 cactggtatg ccgactaaac gctttgataa aaaacactgg aagatggtgg tggtgctact
      181 ggcaatctgt ggcgctatgt tgttgctacg ttgggcagca atgatttggg gctgagcagt
      241 gacaaacgaa aacagccaga ccgcataaca cggtccggca gataatttta catgctgtaa
      301 cccaacgata aggttgtccg acgatcggta tgttccggtg cagactctgg aggttcagag
      361 ttccatgtaa cgttgtaagc caccttcagt ccaaaatgtt cattaatagc aacatttaat
      421 gcgctttcgg agttcagcgt tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat
      481 ttagcgttgt cagtcaactg ccatgcataa gccccggagg catagcccag cggctgggtt
      541 tcactggcat tgtcggtata tttgtcgtaa cgcacaccag ggccgaattc aaagcggaaa
      601 ctgtgtaccg ggccattcaa aaactggcga ccataacccg cggtcaacac atcgcgctct
      661 cgatagccgt tataacggtc tgtcagccag cttgcctgac caaataaata gtcataatca
      721 gttaaattaa aacggctacg cccgcccgcc gcatattttt ctgaagaacg ctcatcatta
      781 gaagaagtat tactggcgtt cccccacagc gaccaggcag tggtttgtcc ataccaggtc
      841 atagtggtgt cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc aagatatcct
      901 gcgttcagat taccttcgaa aggtttttta gcgctggcag gatcgtccat gacagtaaaa
      961 acggaatcat cggcagctgc attcagtgac gcaaacatgc cccccgccaa cataacgatg
     1021 gcaggaactg tcttcaaaag cttcatttat caagagtccg tacaacaaaa aaagagacca
     1081 tcgcggtccc ggaaactttc ttaaggatca aagattagcg tccctggaaa gataacgaat
     1141 tataaaaagg cgcgaataac ttagcaatgt attcttattt catt
//
LOCUS       DADTFG010000347         1167 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.349, whole genome shotgun sequence.
ACCESSION   DADTFG010000347 DADTFG010000000
VERSION     DADTFG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.349"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>1167
                     /locus_tag="J1138_004779"
     CDS             <1..>1167
                     /locus_tag="J1138_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456441.1"
                     /translation="GELTLLSSRNIKPVSGLWRWLFSRCIVTGRPENKRDLDDITFDF
                     FGEAYDALNDKNISIFRTGIDRLTDTYTSIKRSYNYGVDKNYLDEVKESGFSHTFSDS
                     FHYELRKFFRESVKSTEYSGEYFRESMAIPLHVYRKTQSTCFTDFRQFLLSLFRVWHV
                     LNEWKAGLGSPLSASQELTHQALIREYIGLWEGWSMSTITGKPGSEDSTDRLMYHLHN
                     TARLLIPSVVADNASSVRYAHDVLCLWFNQSRFTRYWEEEYRWHSFFLTPDYLSLKET
                     DSQWDMLLRGSLYKKDAALSIIFSNALSDFRLLMAGYLVAHFESQKNIDLADLVNHLI
                     MSELYEDRDTHDTLTPAFRCSVDIIDMILRIEHCNLHTNTSWYSGLSETIEVMNS"
BASE COUNT          319 a          235 c          275 g          338 t
ORIGIN      
        1 tggtgaactg actttattgt catcgagaaa tatcaagcct gtttcaggat tgtggcggtg
       61 gttatttagc cgttgtattg taacagggcg ccctgagaac aaaagggatc tggatgatat
      121 tacttttgat ttctttggtg aggcttacga tgcacttaac gataaaaaca ttagcatctt
      181 cagaacaggg attgacagat taacagatac ctatacctct atcaaaagaa gttacaatta
      241 cggggtcgat aaaaattatc tggatgaagt aaaagaatca ggttttagcc atactttcag
      301 tgattcgttc cattatgaat tgagaaagtt tttccgtgaa tccgtaaaat caacggaata
      361 ttcaggcgaa tatttccgtg aatcgatggc cattccattg catgtgtaca gaaaaacaca
      421 gagcacgtgt tttaccgatt ttagacagtt tttactttcg cttttcaggg tgtggcatgt
      481 gttaaacgag tggaaagcgg ggctggggag ccccctctcg gccagccagg agctgactca
      541 ccaggccctt atccgcgagt atatcggact gtgggaaggc tggagtatgt ccacgatcac
      601 tggcaaaccc gggagtgaag attctactga ccggctgatg tatcatctgc ataataccgc
      661 caggcttctt attccgtcag tggttgccga taatgcctct tccgtgcgat atgctcacga
      721 cgttctctgc ctttggttta atcagagccg gtttacacgg tactgggagg aagagtaccg
      781 ctggcacagt ttcttcctga ctccggatta tctgtccctg aaggagaccg attcgcagtg
      841 ggatatgtta ctcaggggca gcctgtataa aaaagacgcg gcattgtcca ttatattttc
      901 aaatgcctta tctgattttc ggctgttaat ggcagggtat ctcgttgcgc attttgaatc
      961 gcaaaaaaat atagacttag ccgatcttgt gaaccatctc attatgtctg aattatatga
     1021 agatagagat acacatgaca cgctcacgcc tgcattcaga tgttctgtcg atatcattga
     1081 catgatactg agaattgaac actgtaatct gcatacgaat acaagctggt acagtgggtt
     1141 atccgaaaca attgaagtga tgaactc
//
LOCUS       DADTFG010000348         1144 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.350, whole genome shotgun sequence.
ACCESSION   DADTFG010000348 DADTFG010000000
VERSION     DADTFG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.350"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..213)
                     /gene="mchB"
                     /locus_tag="J1138_004780"
     CDS             complement(<1..213)
                     /gene="mchB"
                     /locus_tag="J1138_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HBA8456442.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSA"
     gene            complement(230..439)
                     /gene="mchI"
                     /locus_tag="J1138_004781"
     CDS             complement(230..439)
                     /gene="mchI"
                     /locus_tag="J1138_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HBA8456443.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
BASE COUNT          383 a          244 c          214 g          303 t
ORIGIN      
        1 agcagaagaa ctggcactac cacttcccac ggtcgagcca attgctgttg tcaaaccggc
       61 agatacggct ccaactacaa ccccaagtgg accaccaggt attccggcga gagctccaac
      121 aatagctgca gcacctgcag cattagctga agtcgctggc gcacctcccg ccccggaaat
      181 atatcttaac tgtgattctg ttatttctcg catataaact ccatcatatt taacttccgt
      241 ttttccagcc tgaccagacc agaatccttc gaatcccatg aatcaactca ataaaaccaa
      301 gaaatattat aaagcttaga aaaacgtcaa cataagaatt cccttcgcca acaatccgaa
      361 gggatgaaag tgaaaccaat aagatagtaa taggtaaact gaatatagcc gtcaattggg
      421 acagtttttt ataactcatt aatacctacc agttgcttta ttttcattat gaaaagttac
      481 caaatcttag cacctcagtc aggagagtca ggtcatcaat aaagaatatt ccagtagagt
      541 ggcgctcatg atcaaagata acagcaaagt tgatgacagt gctgaccgac tgtcatttac
      601 agaaaccctg tttgtagcaa attccatctt acctgacctc acccagacaa agacaaaaga
      661 gaatgattgt tatttacttt tcaaaaaaag tgtcatattg tccatataaa gtcaagaggt
      721 attttacgtt atgtcactga tgcaattgtt agtcaggatc tcatggttgt cgctcatgta
      781 taacctgaga cttgaacgca cactaatagc actgacggtc atacatcggg aaatgtgagg
      841 aggcataaga atgaattgtg ataataatca cagaaatgaa gaattcattg ttacctttaa
      901 taaaggcaac aagcaagaca attcaagacg aaaacacgat aattttccta tagaggtaga
      961 atcctccgta gagcaggaga cacactgtat cacaaataat aagtcggctt ccggtatagt
     1021 aacacatgac tatgatgccg attatatttg tggttgtggt gaaattatgt gtcctggttg
     1081 cggtcatgac ctataacaaa catttttgca aactgatacc tgatcaggta caagcctgtt
     1141 gatt
//
LOCUS       DADTFG010000349         1142 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.351, whole genome shotgun sequence.
ACCESSION   DADTFG010000349 DADTFG010000000
VERSION     DADTFG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.351"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..376
                     /locus_tag="J1138_004782"
     CDS             <1..376
                     /locus_tag="J1138_004782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA primase/helicase"
                     /protein_id="HBA8456444.1"
                     /translation="KVDENVPTGKMDVKGTGAITDMADNLFSVWRNIPRETAQQKQDD
                     PSAPELTDKDRAALALPGTLIRLLKQREGEGWVGDIGAFLEPRSHQFLENEKGSPWNY
                     LVAREQAEVDMDWEMQNVTRAC"
     gene            376..687
                     /locus_tag="J1138_004783"
     CDS             376..687
                     /locus_tag="J1138_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456445.1"
                     /translation="MDRLIREMSYLFTKSRFEELQERAEEISIEYCWHIECFGLVADA
                     IEEFVEETPADEWREHEKIIMHYMVMRGLALYGDGEKITKIQWAHPGWMGTAEKGDTI
                     Q"
     gene            684..869
                     /locus_tag="J1138_004784"
     CDS             684..869
                     /locus_tag="J1138_004784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456446.1"
                     /translation="MNLTREDEHTITQYIRAAHGGYTGPVALWMKRLEELHMPFSRLV
                     VMTAMMKAKHESKRAAQ"
     gene            866..>1142
                     /locus_tag="J1138_004785"
     CDS             866..>1142
                     /locus_tag="J1138_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004957921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBA8456447.1"
                     /translation="MKLESALKHFSAQGLTITDAPNGTSADRITGTDVMAALGLAESK
                     ARFGMAAFLGKTGISSEDRERAIAELTQYAMRRAPKHVGKVAGRRMAR"
BASE COUNT          298 a          280 c          349 g          215 t
ORIGIN      
        1 caaggtggat gagaacgtgc ccaccggcaa gatggacgtc aagggcaccg gcgccatcac
       61 cgacatggcc gacaacctgt tctctgtctg gcgcaacatc ccgcgcgaga ctgctcagca
      121 aaagcaggat gatccgagtg cgcccgaact cacggacaaa gacagggcgg cactggcatt
      181 gccaggcacg cttattcgcc tgttgaaaca gcgtgagggg gaagggtggg tcggcgacat
      241 tggcgcgttt ttagagccac ggtctcacca atttttagaa aacgaaaaag gcagtccatg
      301 gaactacctt gtcgcgcgcg agcaagccga agtggacatg gactgggaaa tgcagaacgt
      361 aacgagggct tgctgatgga tcgcctgatt cgcgaaatgt cttatctgtt cactaaatcc
      421 cgatttgaag agctccagga gcgagcggag gaaatctcca ttgaatactg ctggcacatc
      481 gagtgtttcg gtttggtagc ggatgccatt gaggagtttg tcgaagagac gccagcagat
      541 gaatggcgtg aacatgaaaa aattatcatg cattacatgg tcatgcgtgg attagccctg
      601 tacggagacg gtgaaaaaat taccaaaatc caatgggcgc accctggctg gatgggcact
      661 gctgagaaag gagacaccat ccagtgaatc taacccgcga agatgagcac accatcaccc
      721 aatatatccg cgcagcacat ggcggataca cagggccggt ggcgttgtgg atgaagcgtc
      781 tggaggaatt acacatgccg ttcagtcggc tggtggttat gacggcgatg atgaaggcga
      841 aacacgaatc gaagagggct gcacaatgaa acttgagtcg gcattaaagc atttcagcgc
      901 gcaagggctg accattaccg acgcaccgaa tggcacctca gcggatcgca ttactggcac
      961 tgacgtcatg gccgctttgg ggcttgcgga atcaaaagcc cgctttggca tggcggcgtt
     1021 cctgggtaaa acggggatca gcagcgaaga ccgggaaagg gcaattgcag agttgacgca
     1081 gtacgcgatg cgcagggccc ctaagcacgt cgggaaagtc gccggtcgtc gcatggctcg
     1141 ct
//
LOCUS       DADTFG010000350         1136 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.352, whole genome shotgun sequence.
ACCESSION   DADTFG010000350 DADTFG010000000
VERSION     DADTFG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.352"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(83..1117)
                     /locus_tag="J1138_004786"
     CDS             complement(83..1117)
                     /locus_tag="J1138_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBA8456448.1"
                     /translation="MAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYVR
                     LGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRN
                     YGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQ
                     GKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQVIYGNNSLNASGQNAE
                     VWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQFDFGLRPSVAY
                     LQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGVATDD
                     IVAVGLVYQF"
BASE COUNT          311 a          259 c          249 g          317 t
ORIGIN      
        1 gcttctaagg aaagtggctt gagggggcca cttatatgtt ggggaggcaa agcctcccac
       61 aacatatctt tgagtaatca gattagaact ggtaaaccaa tcctacagca acgatatcat
      121 cggtagcaac gccagatgct ttcgtgaaat cgctcttatc aatcaggttg attttgtaat
      181 caacaaaagt ggacatattt ttgttgaagt aataggttgc acctacatca atatattcaa
      241 ccaggtcctg atcaccccat gcacccaagt cttttccttt agattgcagg taagcaacgg
      301 acggacgcag accgaagtcg aactgatatt gtgcaactac ttcgaagttt tgtgctttgt
      361 tggcaatatg gttattacca aaaacagtca tgttctgggt ttcagaatag gtggtagcca
      421 gatagatgtt gttcgcatca tatttcagac cagctgccca tacttcagca ttttgaccag
      481 atgcattcag gctgttgtta ccgtagataa cctgatcatt agtgcggtca gatttagcat
      541 aggttgcacc tacaccgaat ccttcatact cataagtagt ggagaaaccg aaaccatcac
      601 cgttagcttc agttacgtca gtgcggtcat ttttaccctg atactgagca gcaaagttca
      661 gaccatcaac cagaccaaag aagtcgttgt tacgataggt tgcaacacct gtggtgcgac
      721 cagtcatgaa cacatctgtt tgggtccagg tatcgccacc gaattctggc agaacgtcgg
      781 tccatgcacc gatatcgtat gctacaccgt agttacggcc ataatcgatg gagccgtagt
      841 caccgaattt caggccagcg aaggcaagac gggttttatc tttggaagaa ccttgagatt
      901 cagcacggtt gcctttgaat tcatattccc actgaccgaa accagtcagt tgatcgttga
      961 tttgagtttc gcctttgaag cccagacgaa cataagttgt gtcgccatca tctgcatcgt
     1021 tagaagagaa gtagtgctta gcattaactt ttccgtacag atccagcttg ttactgtctt
     1081 tattgtaaat ctctgctgcc tgagcagaca tcgccatcag tactgatgca gctaca
//
LOCUS       DADTFG010000351         1134 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.353, whole genome shotgun sequence.
ACCESSION   DADTFG010000351 DADTFG010000000
VERSION     DADTFG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.353"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            402..857
                     /locus_tag="J1138_004787"
     CDS             402..857
                     /locus_tag="J1138_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBA8456449.1"
                     /translation="MVAEGILLEGKEWLITRCEKIYFDNLEKWFGHYCHIHCQVSLGR
                     LINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKIVGVIELDDPSHERADRIIR
                     DHWLDTIMTLARVPFVRASVTKLEEIPPVWDTRDKIRPLVAATKSEPLH"
BASE COUNT          298 a          236 c          279 g          321 t
ORIGIN      
        1 tgttgttatt ttttgtggaa ggcaagccgc aatggcaaaa taggggtggt tgtaataata
       61 aaaacatgat actaactata tattgatcgt tgaattgttg gcatgtaaca cgctacagcg
      121 agtaacgttg gtaatgaaat ataatcaaca tggataggtg aaaattaatt atgagcttta
      181 tggcttttgt aacttgtttg acaattgcat ttgtcgccgg ttttgttgtt gccagaaaat
      241 tgacactccg aaaagttgcc tcatcggtga tcatctctaa atcgcaacca gtcccttcat
      301 ctgcaactcc ccctgctgct aaggcgtcga caaggagcta tacaccgcag aaaacctatc
      361 gtgattctcg ttggcaagaa cgagatgagt tatttcaggg aatggttgcc gaaggcattt
      421 tattggaagg aaaagagtgg ttaattacgc gttgtgagaa aatctatttc gataatctgg
      481 agaaatggtt tggacattat tgccatatcc attgccaggt ctctctgggg cgcttgatca
      541 actttccaga acaacctgag tttagcgaag aagaaagaaa gcgtttcttt gctatttata
      601 atggcatggc aatggattat gtgctggttt caaagaagaa caataaaata gtcggtgtta
      661 ttgagctgga tgatccgagt catgaaaggg cagatcgcat tattcgtgac cattggttgg
      721 atacgattat gactcttgct cgggtgcctt ttgtacgagc ttctgtcact aaattggagg
      781 aaatccctcc ggtctgggat acccgtgaca aaattcgccc attagtcgcg gcgacaaaga
      841 gtgaaccgtt gcattaacca ggtatccccg gtcggggctt ctcatccccc cggtgtatgc
      901 aatagacgaa aaaaagctcg cactttcgta cgagctcttc tttaaatatg gcggtgaggg
      961 ggggattgac tcgcttcgct cgccctgcgg gcagcccgct cactgcgttc acggtctgtc
     1021 caactggctg tcgccagttg tcgaaccccg gtcggggctt ctcatccccc ggtgtgtgca
     1081 atatacgaaa aaaaagcccg cactttcgta cgagctcttc tttaaatatg gcgg
//
LOCUS       DADTFG010000352         1126 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.354, whole genome shotgun sequence.
ACCESSION   DADTFG010000352 DADTFG010000000
VERSION     DADTFG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.354"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            112..399
                     /locus_tag="J1138_004788"
     CDS             112..399
                     /locus_tag="J1138_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006318559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA8456450.1"
                     /translation="MANVELAPEVRDDIERILAHLAQYGAADSGRRIQEIIQAIAVLE
                     HNPLIGRSLGAGLRELIIGQQARGYLALYKYIPDIDTAFVLAIRSQKEQGY"
     gene            complement(443..>1126)
                     /locus_tag="J1138_004789"
     CDS             complement(443..>1126)
                     /locus_tag="J1138_004789"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="B2 or R2 protein; type 1b enzyme; catalyzes the
                     rate-limiting step in dNTP synthesis; converts nucleotides
                     to deoxynucleotides; forms a homodimer and then a
                     multimeric complex with NrdE; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBA8456451.1"
                     /translation="NISFMEAVHARSYSSIFSTLCQTPDVDDAYRWSEENRALQKKAG
                     IILAHYRSDDPLLKKVASVFLESFLFYSGFYLPMYWSSRAKLTNTADLIRLIIRDEAV
                     HGYYIGYKFQKGLEKVDAARRQQVKNFAFDLMQDLYDNEVRYTEELYDGVGWTEDVKT
                     FLHYNANKALMNLGYEALFPPAMAEVNPAILSALSPNADENHDFFSGSGSSYVIGKAV
                     NTEDEDWDF"
BASE COUNT          237 a          293 c          334 g          262 t
ORIGIN      
        1 aattgcgacg ggagagacgg tctcttggaa agagatgcgc ggttatttgg aaaattacgc
       61 agcgggtgat actgccgctg tcaaacctcc ggttaaaaaa ttggggcgct gatggcaaat
      121 gtcgagttgg cgccagaggt cagggatgat atcgaacgta ttctcgctca tttggctcag
      181 tatggcgctg cggattcagg gcggcgcatt caggaaatca tccaggcaat tgcggtattg
      241 gaacataatc cattgatcgg acggtccctt ggcgcagggc tgcgtgaatt gattattggg
      301 cagcaagctc gcggttattt ggcgctctac aaatacattc ctgatatcga tacggcgttt
      361 gttctggcga tccgcagtca gaaagaacaa gggtattgaa tgcacgatac cggcctgaaa
      421 gaccggcatc gctgtcatgt cctcagaagt cccagtcctc atcctcggtg ttgaccgcct
      481 tgccgatgac gtaggacgat cctgaaccgg agaagaagtc gtggttttcg tcggcgttcg
      541 gcgacagcgc cgacaggatc gccgggttga cctccgccat cgccggcggg aacagcgctt
      601 catatcccag gttcatcagc gccttgttgg cgttatagtg caggaaggtc ttcacgtctt
      661 ccgtccagcc gacgccgtcg tacagttcct cggtgtagcg cacctcgttg tcgtacagat
      721 cctgcatcag atcgaaggcg aaatttttca cctgttggcg ccgtgcggcg tccactttct
      781 ccagcccttt ctggaactta tagccgatat agtagccgtg caccgcctcg tcgcgaatga
      841 tcaggcggat cagatcggcg gtgttggtca gcttggcccg gctggaccag tacatcggca
      901 ggtaaaagcc cgaatagaac aggaacgact cgaggaacac gctggcgact tttttcagca
      961 gcggatcgtc gctgcggtag tgcgccagaa tgatgccggc tttcttctgc agcgcgcggt
     1021 tttcctcgct ccagcgatag gcgtcatcca cgtccggcgt ttggcacagg gtagagaaga
     1081 ttgagctgta agagcgggcg tgcaccgcct ccataaagct gatgtt
//
LOCUS       DADTFG010000353         1092 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.355, whole genome shotgun sequence.
ACCESSION   DADTFG010000353 DADTFG010000000
VERSION     DADTFG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.355"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            131..>1092
                     /locus_tag="J1138_004790"
     CDS             131..>1092
                     /locus_tag="J1138_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HBA8456452.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPTPPKPISFNNDVILDKTEKTLTIRD
                     SVFTYTENADGTISLQDSNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGEL
                     VITGDNTTVNNTGKTIVDGKGATGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSATV
                     DNKGGMTVTDPDSIGIQIDGDKAVVNNDGDSAISNGGTGTQVNG"
BASE COUNT          322 a          249 c          269 g          252 t
ORIGIN      
        1 cagattgtca gagaatatgt ttgattaggt aacaatctac tggcaatatt ggacgtcctc
       61 tatgttttaa ataatcaatt ggtcgggtca gggtatccga ccttctttat attcgccaga
      121 aggatttatt atgcaaagga aaactctatt gtcggcctgt attgcattag ctctgagtgg
      181 tcagggttgg gcggcagata ttacagaaat agaaaccacc acaggtgaaa agaaaaatac
      241 caatgtgact tgtccagcag atcctggaaa actcagtcca gaagagctta aacgcttacc
      301 ctctgaatgc tcctctgtag tcgaacagaa cctgatgcca tggcttgtca caggtgctgc
      361 tactgcgtta atcacaacct tagccatagt ggaactaaac gacgatgatg atcatcatcg
      421 caataattct ccacttccac caactccacc tgatgatgat tcagacgaca ctccagttcc
      481 tccaactcct ggcggagatg agataatacc agacgatggt ccggatgata ccccgacgcc
      541 tcccaaaccg atttcgttta ataatgacgt tatcctcgat aaaacagaaa aaacgttaac
      601 tattcgcgat tcagttttta cttataccga gaatgctgac gggactattt ctctgcaaga
      661 tagcaatggg cgtaaggcga ctattaatct ttggcagatt gatgaaacga ataacacggt
      721 tgcccttgaa gggatgagcg cagatggtgc aacgaagtgg caatataatc acaacggtga
      781 gctcgttatt acgggcgaca ataccacagt aaacaacact ggaaaaacca tcgtcgacgg
      841 caaaggcgcc accggaactg aaatcgcggg taataacgcg gtggtgaacc aggacggcga
      901 gctggatgtg agcggcggcg gtcacggtat tgatatcacc ggcgacagcg cgacggtgga
      961 caacaaaggc ggcatgactg tcaccgatcc ggattcgatt ggtattcaga tcgacggcga
     1021 caaggcggtt gtcaataacg acggtgacag tgccatcagt aacggtggca ccggcacgca
     1081 ggttaacggt ga
//
LOCUS       DADTFG010000354         1075 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.356, whole genome shotgun sequence.
ACCESSION   DADTFG010000354 DADTFG010000000
VERSION     DADTFG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.356"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            241..771
                     /locus_tag="J1138_004791"
     CDS             241..771
                     /locus_tag="J1138_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA8456453.1"
                     /translation="MKKILLVAGAALALAGCGEKGDFEKAINAKIGQDKYCFSLDNNN
                     TSFPIRLAKPRLDSTGTGTNSVILDGFVEQGMMVFEQGYDSNVLGITEEGKKAKVWST
                     TDGACVGRRAVDGIKEWTEPGNGNQKVVRVTYTWKLVDVPGWIDKKAFASVKGMNEPA
                     DGAMNLFKTSNGWKAN"
     gene            782..1057
                     /locus_tag="J1138_004792"
     CDS             782..1057
                     /locus_tag="J1138_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456454.1"
                     /translation="MENNTIKCPFCFKESQRGVHVCTGCLATVLYGTYPGWYAAVVLL
                     LTFGLSTLIGMSTGMAGATISLPIIAVVGFIAGKAIFSDNVVFRRKI"
BASE COUNT          303 a          185 c          283 g          304 t
ORIGIN      
        1 tattgatcgg ttattaattg tttttatcga tcgaatattt atttatgata gctagtggct
       61 atcataagat tgtttttgat cgttttaaac gatatgacga gacgctaaaa gaattgaatc
      121 ctatcaattg gttaagcggt ggttttgcca tgtgatatca gccggatccc gcaacaactc
      181 tagccctgac cagatttggt caaaaattta ggcactttga ttatcaggat ggataagcag
      241 atgaagaaga ttttactcgt tgctggcgca gctctggcac tggcgggatg tggtgagaaa
      301 ggtgattttg agaaggcgat caatgccaag atagggcagg acaaatattg cttctcgctg
      361 gataacaata acaccagctt ccccatccgt cttgccaagc cacgacttga ttcgactggt
      421 acagggacga attcagtgat cctcgatgga tttgtagagc agggaatgat ggtctttgag
      481 cagggctatg attcgaacgt attagggata acagaggaag gcaaaaaagc caaagtctgg
      541 agcacaacag atggtgcctg tgttggtcgt cgtgctgttg acgggattaa ggaatggact
      601 gagccgggaa acggtaatca gaaagtagtt cgcgtgactt atacgtggaa gctggttgac
      661 gttccgggct ggattgacaa aaaagcattc gccagcgtta aaggaatgaa tgaacctgct
      721 gatggtgcca tgaatctctt taagaccagc aatggctgga aagccaatta aggaaaccaa
      781 tatggaaaac aataccatca aatgcccgtt ctgtttcaaa gagagccagc gtggggttca
      841 cgtttgcaca ggttgcttag cgactgtgtt atatggcact tatcccggct ggtatgctgc
      901 tgttgttttg ttgttgactt ttggtttatc gacgttgatc ggtatgtcaa caggtatggc
      961 aggagctact atcagtctac caataattgc ggttgttggg tttatagctg gtaaggcgat
     1021 attctctgat aatgttgtgt ttaggcgaaa gatataaaaa acgaaaagaa ttata
//
LOCUS       DADTFG010000355         1057 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.357, whole genome shotgun sequence.
ACCESSION   DADTFG010000355 DADTFG010000000
VERSION     DADTFG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.357"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            221..694
                     /locus_tag="J1138_004793"
     CDS             221..694
                     /locus_tag="J1138_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004950924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBA8456455.1"
                     /translation="MKQITAHELTAMSEQAASVARLRAHRTLHEELSDPVQRLAIAME
                     PGTYIRPHRHPQTWELLTPLRGRFVVLQFNDDGVVTQRTLLGEETKVLEMPAFTWHAV
                     LSVDAGGVVFEVKQGPYQALTEEDCMQWAPQEGGEGTEAVMRWYATAQVGDRYPH"
     gene            complement(738..>1057)
                     /gene="uvrD"
                     /locus_tag="J1138_004794"
     CDS             complement(738..>1057)
                     /gene="uvrD"
                     /locus_tag="J1138_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462836.1"
                     /note="unwinds DNA duplexes with 3' to 5' polarity with
                     respect to the bound strand and initiates unwinding most
                     effectively when a single-stranded region is present;
                     involved in the post-incision events of nucleotide
                     excision repair and methyl-directed mismatch repair;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBA8456456.1"
                     /translation="ETRRLYGKEVYHRPSRFIGELPEECVEEVRLRASVSRPVNHRRM
                     GTPISENDTGYKLGQRVRHPKFGEGTIVNLEGSGEHSRLQIAFPGEGIKWLVAAYARL
                     ETV"
BASE COUNT          231 a          324 c          310 g          192 t
ORIGIN      
        1 cgtctgtcct caatgataat agtgcgcgca tgttacgccg agaagaaaaa gcctgtcaac
       61 acgttaggcg ggataattgc gcaacaaatc atcacgatga tcaatagcag aaatggggct
      121 taaaccgtac tgaaaaagtc gcggcgatcg cttaccgcca ccaagaatgt tgctaagatg
      181 acatcaccct aatggtacga caagcatgcg gatttaaatg atgaaacaga ttaccgccca
      241 tgaactgacc gcgatgagcg aacaggccgc cagcgtggcc cgcctgcgcg cgcaccgcac
      301 cttacacgaa gagctgagcg acccggtgca acgcctggct atcgccatgg aacccggcac
      361 ctatatccgc ccgcaccgcc acccgcaaac ctgggagctg ctgacgccgc tgcgcggccg
      421 ctttgtggtg ctgcagttca acgatgacgg cgtcgtaaca cagcgcaccc tgcttggcga
      481 agaaaccaaa gtgctggaga tgccggcctt tacctggcat gcggtgctgt cggtcgatgc
      541 cggcggcgta gtgttcgaag tgaagcaggg cccgtatcag gcgctgaccg aagaagactg
      601 catgcagtgg gcgccacagg aaggcggtga aggcaccgaa gcggtgatgc gctggtatgc
      661 caccgcacag gtcggcgacc ggtaccctca ctgatcgtga aaggttgcag gggctggccc
      721 ctgcaacgcc tctctcttta taccgtttcc aaacgcgcgt aggccgccac cagccatttg
      781 atgccctcac ccgggaaggc gatctgcagc ctgctgtgct cgccgctgcc ctccagattg
      841 acgatggtgc cttcgccaaa cttgggatga cgcacgcgct ggcccagctt gtaaccggtg
      901 tcgttctcgc tgatcggcgt gcccatgcgg cggtgattga ccgggcgcga aacgctggcg
      961 cgcaggcgca cttcttccac gcactcctcc ggcaattcgc cgataaagcg cgacggccgg
     1021 tgataaacct ctttgccgta cagacggcgc gtttcag
//
LOCUS       DADTFG010000356         1041 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.358, whole genome shotgun sequence.
ACCESSION   DADTFG010000356 DADTFG010000000
VERSION     DADTFG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.358"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(195..350)
                     /locus_tag="J1138_004795"
     CDS             complement(195..350)
                     /locus_tag="J1138_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456457.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(430..495)
                     /locus_tag="J1138_004796"
     CDS             complement(430..495)
                     /locus_tag="J1138_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456458.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(498..686)
                     /locus_tag="J1138_004797"
     CDS             complement(498..686)
                     /locus_tag="J1138_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA8456459.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(697..909)
                     /gene="cspI"
                     /locus_tag="J1138_004798"
     CDS             complement(697..909)
                     /gene="cspI"
                     /locus_tag="J1138_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HBA8456460.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          348 a          205 c          177 g          311 t
ORIGIN      
        1 gtttttaagt cttcaatatt catcatttcc tcttttagtc tgttatgact ttccagttac
       61 acagtaagtc gattatatgg tgcaaacgtg taaaagataa gatgaaacat cgcaataatc
      121 aacatacgat agtctaaatt ttacacaaac agacaaagag aattttcctg aattatcaat
      181 acaatagcat caaatcaact caagagcctt attgctgctt ccagaatttc ttctgaagta
      241 acatgtcgat ccgcggctac ataaatgact ttatgatctc cggtcagaga tggaaaccct
      301 gcggccatta cagtaaggtg tgttttttcg ccatttggat attcacgcat gatggtgtta
      361 actccagtca tcgctggcac taccactgct ggttcagagt taaaaaaaac tatgattttt
      421 ttcatgatgt taccgtagta tgtgagtatc catcgaatag acaccaagca aaaaagctcc
      481 cgaaggagcc ttcattttca cttttttaaa tccaacgaca gacggctggc atttaagtat
      541 tgtgaaatat tatcaaatgt aatcatcatt gatttacaaa agatacattt tgccccgaaa
      601 ggattcatgt cagaaacatc aaaagatgat gttctatact gggaaccatg acaacacggg
      661 catctaaagt gaatatggtt tgtaatattg tctacctcaa agcgccacta catgaacagc
      721 ggcaggacct ttaggtccgt tctcaatacc aaattcaact tcctgattct cagttaatgt
      781 tttgaaatcg ttgctctgaa ttgctgagaa atggacaaac acatctttgc tgccatcttt
      841 cggcgtgatg aaaccaaaac ctttttcagg gttaaaccat ttcactaaac cagtcatttt
      901 gttagacata attattacct tttgaagaaa ttagcccttg ggcagaatgg tccgaaaaaa
      961 atatcagaga gaaaaaccaa caaggaaatc ccaagaggta caaataataa aattatcaca
     1021 atgactgctt cagataaatt t
//
LOCUS       DADTFG010000357         1018 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.359, whole genome shotgun sequence.
ACCESSION   DADTFG010000357 DADTFG010000000
VERSION     DADTFG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.359"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(194..826)
                     /locus_tag="J1138_004799"
     CDS             complement(194..826)
                     /locus_tag="J1138_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBA8456461.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          265 a          225 c          202 g          326 t
ORIGIN      
        1 ataaaatata acataataaa tgatttgtga taatatggga ttattgcggt ttactttgag
       61 gaagataaaa tgcgagttta tagaaccgcg agcgccaaat aattttatga ccacaacctg
      121 tcgtattgtg gtcataaaaa attgtcgact ggagggagtt aaatccttta cctcaatata
      181 ttgcctgtca ggtttagttc acaaacagtg cttcgctatc aacgatgatt ccatcttccg
      241 cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt ttctgcgcat
      301 cagcaagtgc attacgagtc tgtttaagcg gcatcaaata ttggttttcc attacaccaa
      361 caccggccag gcgaagctct tccattgcac cctttttatc ccctttcgcc attttttcgt
      421 tggcaatttt tatcgcggct tctttttcgg gtgtcgctac ataactttca gaaattccga
      481 cagatgcatt aatgacaata tattggtcat cattgacgtt ggtcttttta cctggcttag
      541 cgaactttgc ccattcggta ctatcatctg acagcaaagc ggaagcttca ttggtcagtt
      601 cttttgcctt ttccggatcg ccatgaaaca gagccaggcg cgctacctgc acgtcacgca
      661 tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg gtttgcttaa
      721 cttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat acagttgaag
      781 atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt acctcattct
      841 ttaacagttt tagaaaacgc cgccatcata ttgggtgaga atgaaatgag aaatcagaga
      901 gcaacggaca ttttgaaagg gttatcttac aactgtagtt attatcgcaa agccgctttt
      961 agtcttacag ttgtaggaat ctgcttcctg tcgcagagaa ctattcttat ataccaca
//
LOCUS       DADTFG010000358         1009 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.360, whole genome shotgun sequence.
ACCESSION   DADTFG010000358 DADTFG010000000
VERSION     DADTFG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.360"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            106..261
                     /locus_tag="J1138_004800"
     CDS             106..261
                     /locus_tag="J1138_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA8456462.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLIAKAPLEGWHYGTKEIVKKFH
                     MYHRVAI"
     gene            346..636
                     /locus_tag="J1138_004801"
     CDS             346..636
                     /locus_tag="J1138_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA8456463.1"
                     /translation="MYNFKDKIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVKEWRRSQGLPLFKDSK"
     gene            803..991
                     /locus_tag="J1138_004802"
     CDS             803..991
                     /locus_tag="J1138_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA8456464.1"
                     /translation="MSQYPELIAQFSTGNQTRIKQGLIAKAPLEGWHYGSKEIVKEFH
                     IYHSVAIECGGEIYDIDN"
BASE COUNT          350 a          160 c          208 g          291 t
ORIGIN      
        1 cataaaataa taatgtgatt gctcttaaaa aacaggtaaa aatactgaag cctcattagg
       61 ttattttatc attttcccgc ttttaccgat accctttggc tggaggtgag tcagtatccg
      121 gagttgatag cgcagttttc aagcggtaat caaacacgta taaaacaagg attaatcgca
      181 aaagcaccac ttgaaggttg gcattatggc actaaagaaa tcgttaaaaa attccacatg
      241 tatcatcgtg ttgcgattta atatagtggc ggaatttacg atattgataa tttaaggagg
      301 gcacacctca tttgcatgat gaaatttatt acaggagata gttaaatgta taattttaaa
      361 gataaaatag aagattacac agagcgggaa ttcattgagt tgcttggcga gtttacaaat
      421 ccaaccgggg ataacgctca attaaaaggg gaagagcttg acaaatattg ggatgatcta
      481 gaagaacatc ttaccagaat tacacaacat ccgttgatga gcgatcttat ctactatcct
      541 gcaaagaagg gagatgatga acctgaaaac atcctgaaaa ttgtcaaaga atggcggcgt
      601 tcacaggggc tacccttgtt taaagactct aaataaagta gataataaaa ctatatatgg
      661 aacatcatcg cgtctggcta atgcgatgat gttaataaca taaaataata atgtgggtgc
      721 tcttaaaaaa caggtaaaat actgaaattt cattaggtta ttttatcaat ttcccgcttt
      781 caccgatacc ctttggctgg aggtgagtca gtatccggag ttgatagcgc agttttcaac
      841 tggtaaccaa acacgtataa aacaaggatt aatcgcaaaa gcgcctcttg aaggttggca
      901 ttatggctct aaggaaattg ttaaagaatt ccatatatat catagtgttg cgattgaatg
      961 tggtggcgaa atttatgata ttgataatta aaggattgtt acgcctcga
//
LOCUS       DADTFG010000359         1003 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.361, whole genome shotgun sequence.
ACCESSION   DADTFG010000359 DADTFG010000000
VERSION     DADTFG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.361"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            263..853
                     /locus_tag="J1138_004803"
     CDS             263..853
                     /locus_tag="J1138_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA8456465.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDELIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT          335 a          204 c          197 g          267 t
ORIGIN      
        1 ttccacgacc aaacaacttg aaatctggtt aaaataacac gcaacactat tcttcttcct
       61 tgagtccgcc cggaactcga aaaacaaacc gagttaaggc catttttcac aaaatcgatt
      121 ttgggtctca ccaaaattac ggggttgcat acgcattcgt ttattttcga acgtgtacat
      181 acaaatatgc acaaaaataa tcaaaattat tttctgagat gcattatgat atgtacacca
      241 atttcgtata gaagatctca ctatgtctcg aatttatgct tactgtcggg tatcaactct
      301 ggaacagaca accaaaaatc aacgtcggga aatcgaaagt gcaggtttta acatcaaatc
      361 tcagcaaata attgaagaac aaattagcgg ctcagcagca accagtgagc gtcctggttt
      421 taaccagttg cttgatcgac tgaaaagagg tgatgaattg attgtcacaa aactggatcg
      481 cctcggttgt aatccaatgg atatcagtaa aacggtggaa caactgacca aagcaggtat
      541 caaagtgcat tgcttagcat tggggggcgt tgacctgacc agtccaccag gaaaaatgat
      601 gatgcaagta atttcagcag tcgctgaatt tgagcgagac cttttacttg agcgtactca
      661 ttcagggata ttaagagcac ggggcgcagg aaagcgattt ggtcgcccac ctgtgttaaa
      721 tgatgaacaa atacagaggg tactagagca aattaagtca ggtataaata taagtgacat
      781 tgcccaagaa ttcaaaacct cgcggcaaac cattttaaga gctaaagcaa aaaatcagac
      841 acctgatata taaaaataat ctcggtgtga gatttcttac gttttccaag ccctccttcc
      901 tagccttaaa tggaaagata cttctaatta tagaatttat atatcttacc ctacggtagt
      961 gctggccatt caatatccgg tactactgat gtatcaacac ggt
//
LOCUS       DADTFG010000360          998 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.362, whole genome shotgun sequence.
ACCESSION   DADTFG010000360 DADTFG010000000
VERSION     DADTFG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.362"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>998
                     /locus_tag="J1138_004804"
     CDS             <1..>998
                     /locus_tag="J1138_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HBA8456466.1"
                     /translation="VGWDLWSECLTLPDFENVADILIPMGSNEDPFWQGSARTIFVEA
                     AYRMRNDEDRSYAKLLNTLLSIDLPRLRAYLEGTPAANLVDGKIEKTAISIRSVLTNY
                     AKALRYMQGVEKLGAPFTIRDWMRGVKDNGKNGWLFLTSDSASHKALKPLISMWLSIA
                     SHSLLSMGENPDRRVWIFADELPTLHKLPDLVSIVPEARKFGGCFVLGFQTYSQLEDI
                     YGVKAAATLFGVLRTRFLFSVPDRVGAEFAAGEVGEKEYRKASEQFSYGADPVRDGVS
                     VGKDLERMQLVSYSDIQTLPDLCCYVTLPGPYPAVRLQMVYKKVRQVAHPYEPRKM"
BASE COUNT          203 a          286 c          299 g          210 t
ORIGIN      
        1 gtcggctggg atttatggtc ggaatgtctg acactgccgg actttgagaa cgtcgccgat
       61 atccttatcc ccatgggcag caacgaagat ccgttctggc aggggtcggc ccgcaccatc
      121 tttgtggaag cggcctaccg catgcgcaat gacgaagacc gcagctacgc caagttgctc
      181 aatacgctgt tgtccatcga tctgccgcgt ctgcgtgctt atctggaggg cacgccggcg
      241 gccaacctgg tggacggcaa aatcgagaag accgccatct ccattcgcag cgtgttgacc
      301 aactatgcca aggcactgcg ttacatgcag ggcgtagaga aactgggggc accgttcacc
      361 atccgtgact ggatgcgcgg cgtgaaggac aacggcaaaa acggctggtt gttcctgacc
      421 tccgattcag ccagccacaa ggcgctcaaa ccgctgatct ccatgtggct ctctatcgcc
      481 tcacacagct tgttatcaat gggcgagaac ccggaccgtc gcgtctggat ttttgccgat
      541 gaattaccca ccctgcacaa gctgccggac ctggtgtcca tcgtgccgga agcgcgcaag
      601 ttcggcggat gctttgtgtt gggattccag acctactctc agcttgaaga tatttatggc
      661 gtgaaggcgg cggccaccct gttcggggtg ctgcgcaccc gtttcctttt cagcgtaccg
      721 gatcgcgtgg gtgccgagtt cgccgccggc gaagtcggtg aaaaagagta tcgcaaggcc
      781 tctgaacagt tttcctacgg ggccgacccc gtcagggacg gggtgtccgt cggtaaggat
      841 ttggagcgca tgcagctggt gagctacagc gacatccaga cgctgcctga tttgtgctgc
      901 tatgtgacgc tgccggggcc atacccggcc gtcaggctgc agatggtata caaaaaagtc
      961 aggcaggttg cgcatcccta cgaacctcgc aagatgga
//
LOCUS       DADTFG010000361          997 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.363, whole genome shotgun sequence.
ACCESSION   DADTFG010000361 DADTFG010000000
VERSION     DADTFG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.363"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>997
                     /locus_tag="J1138_004805"
     CDS             <1..>997
                     /locus_tag="J1138_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA8456467.1"
                     /translation="PNTALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQY
                     SGIWDGTFKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPD
                     GFGGTEPRITCNAWLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYN
                     RSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNV
                     TKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAD
                     ISIGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKV"
BASE COUNT          210 a          262 c          333 g          192 t
ORIGIN      
        1 cccgaacacg gcactggtcg gcgtgcaggt ggactcggaa cagttcggca gtcagcaggt
       61 gagccgtaat tatcatcttc gcgggcgcat tctgcaggtg ccgtcgaact ataacccgca
      121 gacgcggcaa tacagcggta tctgggacgg aacgtttaag ccagcataca gcaacaacat
      181 ggcctggtgt ctgtgggata tgctgaccca tccgcgctac ggcatgggga agcgtctcgg
      241 tgcggcggat gtggacaaat gggcgctgta tgtcatcggc cagaattgcg accagtcggt
      301 gccggatggc tttggtggca cggagccgcg catcacctgt aatgcctggc tgaccacaca
      361 gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg atgcgctgta tgccggtatg
      421 gaacgggcag acgctgacgt tcgtgcagga ccgaccatca gataaggtgt ggacctataa
      481 ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc cgctacagct tcagcgccct
      541 gaaggaccgc cataatgccg ttgaggtgaa ctggattgac ccgaacaacg gctgggagac
      601 ggcgacagag cttgttgaag atacgcaggc cattgcccgt tacggtcgta acgtcacgaa
      661 gatggatgcc tttggctgta ccagccgggg gcaggcgcac cgcgccgggc tgtggctgat
      721 taaaacggaa ctgctggaaa cgcagaccgt ggacttcagc gtgggtgcgg aagggcttcg
      781 ccatgtaccg ggggatgtca ttgaaatctg cgatgatgac tatgcggata tcagcatcgg
      841 cgggcgcgtg ctggcggtga acagccagac ccggacgctg acgctcgacc gtgaaatcac
      901 gctgccatcc tccggtacca cgctgataag cctggttgac ggaagtggca atccggtcag
      961 cgtggaggtc cagtccgtca ccgacggcgt gaaggta
//
LOCUS       DADTFG010000362          981 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.364, whole genome shotgun sequence.
ACCESSION   DADTFG010000362 DADTFG010000000
VERSION     DADTFG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.364"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(125..754)
                     /locus_tag="J1138_004806"
                     /pseudo
     CDS             complement(125..754)
                     /locus_tag="J1138_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
BASE COUNT          285 a          207 c          206 g          283 t
ORIGIN      
        1 catatggatt caagtaggca gtttccgcaa actgaaagcc attaactctg ataggccgct
       61 atgagtgggc catttgggtt ttgcccatga tcacagtcat agttctggaa aatggctgtg
      121 ctctttaaat aaatgtcttt aactcctcag tgtttatatt cgccagcatt gaagccttta
      181 aatgtgggaa tgtacgaggt atctggaaat cagtttaaaa cctttggtat attccagcca
      241 tcttggcaag tccccggttc cgaacaacca agcgttacaa tcgtaatcag tactatacca
      301 gttttcaagc aaatgcagcc tgtacggttg aacaatatct gatgtgagtt tttctctggg
      361 gtcaggttca ccagcaaaaa gctcaagcga aaagtgccct gcaagtcctt cagagactat
      421 gacttctcct aaagttgagc cgtatctcgg cccatctaac agcacgatgc agttcatgtg
      481 caaacattcg agcaatggag tgcgcataat ttttgcaaaa ttatggattt tctggagcta
      541 ctgttctata gacaactcca gcttcagggt aaaaaccatg atgccctttc tccgggagga
      601 caagtttccc tactttaacc accacgtcca gtgaaggaat cggcattaac cttttaacta
      661 gttcgtaggt atcagtcagg ttgatttgaa gccattcata gtgagcagtg agtttctttt
      721 gggtattaag aaaatagagg ggcatttgag acataacggt accactcaaa agagtcagta
      781 aatcgctaca tgttaacaca caacgtaaat gttgtggtgt aagcacagtt gtagaggacc
      841 agactgttac ccatcatacc tgctcttagg gcagagcaaa ctcaaaccat caaaatagct
      901 ctggcttttg tgtcatagct gtctcaggtt aagtctgagc taatatatta attaaaacag
      961 tccattcgcc atgtattatt a
//
LOCUS       DADTFG010000363          980 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.365, whole genome shotgun sequence.
ACCESSION   DADTFG010000363 DADTFG010000000
VERSION     DADTFG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.365"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            86..>980
                     /locus_tag="J1138_004807"
     CDS             86..>980
                     /locus_tag="J1138_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8456468.1"
                     /translation="MATVWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIIIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKTFVVGLITSFIFLCPL"
BASE COUNT          275 a          156 c          202 g          347 t
ORIGIN      
        1 aagacaatat aattattgct ggatattgtt gaggctccct aatatttact ttaagggctg
       61 tattagaata acacaggaaa caaatatggc aacagtatgg tataaacaag ttaatccacc
      121 acaacggaaa gctctttttt ctgcatggct tggatatgta tttgatggct ttgattttat
      181 gatgatattt tacattcttc atattataaa agcagatctt ggcattacgg atattcaggc
      241 cactttaata gggacagtgg ccttcatagc cagacctatt gggggcggtt tttttggtgc
      301 catggctgat aaatatgggc gtaagccaat gatgatgtgg gcaattatca tttactcagt
      361 cggaacaggc cttagcggta ttgctacaaa cttatatatg ctcgcagttt gccgttttat
      421 tgttggctta gggatgtctg gtgaatatgc atgtgcttca acttatgcag tagaaagttg
      481 gcctaaaaat cttcaatcta aagctagtgc ttttttggta agtggttttt ctgttggaaa
      541 tattattgcg gcacaaataa tccctcagtt tgctgaagta tatggatgga gaaactcttt
      601 ttttataggc ctgttaccag ttttactagt tctttggatc agaaaaagtg ctccagaaag
      661 tcaggagtgg attgaagata aatataagga taaatcaaca tttttgtctg tcttcagaaa
      721 accacatctt tcaatctcta tgatcgtttt cctcgtctgt ttttgtctat ttggtgcaaa
      781 ctggccgata aacggactac ttccttccta cctagcagat aatggagtta atacagtggt
      841 tatttcaact ctgatgacaa tagcaggttt aggtacactg acaggtacaa tattttttgg
      901 ttttgttggt gataagattg gtgtaaaaaa aacatttgta gttggtctaa taacttcatt
      961 tattttcctt tgtcctcttt
//
LOCUS       DADTFG010000364          971 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.366, whole genome shotgun sequence.
ACCESSION   DADTFG010000364 DADTFG010000000
VERSION     DADTFG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.366"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            55..783
                     /gene="traK"
                     /locus_tag="J1138_004808"
                     /pseudo
     CDS             55..783
                     /gene="traK"
                     /locus_tag="J1138_004808"
                     /inference="COORDINATES: protein motif:HMM:NF010296.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
     gene            783..>971
                     /locus_tag="J1138_004809"
     CDS             783..>971
                     /locus_tag="J1138_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HBA8456469.1"
                     /translation="MANINVLVKRKQYLWLGAVALAVAAAIGGGYYFSQDAELETQQL
                     AEPAPDLTGVVDNTFDDRV"
BASE COUNT          180 a          271 c          334 g          186 t
ORIGIN      
        1 atatcgatcg cagcgagggt gtgacctggc tggccaagtt cgtcgaggta aacgatgaaa
       61 actaaacgtc gctatgcgct gatggttgtc gctctgatgg cctgcttcgg cgcccaggcc
      121 ggcacggtgg atgcgccggt cactatccca ctgcagaacg gcagtcaggc gcatgtttca
      181 ctcagcaaca gcgaacccaa cctgttcaac gtccccggcg atcgcgtgac gttcgtcagc
      241 ggcgtggatg aaagcctggt gaattatgag ccggcggcca acggggggct ggtgctgagc
      301 acgctcaata aaaagccgtt cagctttgtg atcgaaaccg agcggggcat gaactattcc
      361 attcgcgccg tgccgcgtgc cggtgtcggc cgcacgttct cactggtggg ggagagccag
      421 ggcgtttctg ttgccgccaa aggctgggaa gaggggcagc cctacgagtc catgctggtc
      481 tcgctcaaca agtcattgat ggagggccgg ttgccggccg gctatgggca gatgccggtc
      541 acgcgggaag cgctggccgc gccttatggt ttgcgggcgc aggcgcagca ggtctggaca
      601 gggcatcacc tgaaggtggt tcgctttgag gtcagcaatc ccggcagtgc cagccagtgg
      661 gtcaacgaac acgacttctg gcaacggggt gtgcgggcgg tgatgttcga tacgcctacc
      721 cggcagatta tcggcggcgg tcgcatgagc gtgtggatca ccttgtccga gggaggccgc
      781 tgatggcgaa catcaatgtg ctggtcaagc gcaagcaata tctctggctg ggcgcggtcg
      841 cgctggcggt cgccgcggcc atcggcggcg gttactactt ctcgcaggat gcggagctgg
      901 agacacagca acttgccgaa ccggctcccg acctgaccgg ggtggtcgac aatacctttg
      961 acgatcgcgt g
//
LOCUS       DADTFG010000365          968 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.367, whole genome shotgun sequence.
ACCESSION   DADTFG010000365 DADTFG010000000
VERSION     DADTFG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.367"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..>968)
                     /locus_tag="J1138_004810"
     CDS             complement(<1..>968)
                     /locus_tag="J1138_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colicin V synthesis protein"
                     /protein_id="HBA8456470.1"
                     /translation="FTADSVRLRIHLRTLIGSVHGLKGALGKIFCLSLVFETINLVMP
                     IGTQLVMDHAIPAGDRGLLTLICVGLMLFILLRAAVGMVRAWSGLVMSTLINVQWQSG
                     LFTHLLRLPLGYFERRKLGDIQSRFGSLDTLRSTFTTSIVGAIMDGIMVIGVLVMMVL
                     YGGWLSGVVIAFTALYVLMRLLTYGYYRQLSEEALVRNARAGSYFMETLYGIATVKMQ
                     GMAERRIAHWLNLEIDTINTGIRVTKMDMLFGGINTFIAACDQVVILWLGTSLVIDNQ
                     MTLGMFVAFGAFRGQFSDRIGSLTEFLLQLRMMSLHNERIADIALH"
BASE COUNT          230 a          294 c          278 g          166 t
ORIGIN      
        1 ggtgcaaagc gatatcggca atgcgctcgt tatgcaggct catcatgcgc agctgcagca
       61 aaaattccgt cagcgacccg atgcggtcgg agaactgccc ccgaaaggcg ccgaacgcca
      121 cgaacatgcc gagggtcatc tggttatcga tcaccaggct ggttcccagc cacagtatga
      181 cgacctggtc acaggccgct ataaaggtgt ttatcccgcc aaacagcatg tccattttgg
      241 tgacccggat accggtgttg atggtgtcga tttcaaggtt gagccagtga gcgatacggc
      301 gttccgccat gccctgcatc ttgaccgtgg caataccgta cagcgtctcc ataaaatacg
      361 agccggcgcg ggcatttctc accagcgcct cttcggacag ctggcgataa tagccgtagg
      421 tcagcaggcg catcagcaca tagagcgcgg tgaacgcgat caccaccccg ctcagccagc
      481 cgccatacag caccatcatc accagcacgc cgatcaccat gatgccgtcc atgatggcgc
      541 cgacgatact ggtggtgaag gtgctgcgca gcgtatccag cgagccgaag cgtgactgaa
      601 tgtcacccag cttgcgccgt tcaaagtaac cgagcggcag gcgcaacagg tgggtaaaca
      661 ggccggattg ccattgcacg ttgatgagcg tcgacatgac cagtccggac caggcgcgca
      721 ccataccgac ggcagcccgc aacaggatga acagcatcag cccaacgcag atgagcgtca
      781 gcaacccgcg atcgccggca ggaatggcgt gatccatcac caactgcgtg ccgatgggca
      841 tcaccaggtt gatggtttcg aacaccagag acaggcaaaa gattttaccc agcgccccct
      901 tgaggccatg cacgctgcca atcagcgtgc gcaggtgaat gcggaggcga acgctgtcgg
      961 cggtgaat
//
LOCUS       DADTFG010000366          962 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.368, whole genome shotgun sequence.
ACCESSION   DADTFG010000366 DADTFG010000000
VERSION     DADTFG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.368"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            1..762
                     /gene="ydeO"
                     /locus_tag="J1138_004811"
     CDS             1..762
                     /gene="ydeO"
                     /locus_tag="J1138_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBA8456471.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNKMSALAI"
BASE COUNT          291 a          188 c          187 g          296 t
ORIGIN      
        1 atgtcgctcg tttgttctgt tatatttatt catcatgcct tcaacgctaa cattttagat
       61 aaagattacg ccttctctga cggcgagatc ctgatggtag ataacgctgt tcgtacgcat
      121 tttgaacctt atgagcggca ttttaaagaa atcggattta atgaaaatac cattaaaaaa
      181 tatctacaat gcactaacat ccagacagtg acggtgcctg ttcctgcgaa gtttttacgt
      241 gcttcaaatg taccgactgg attgcttaat gaaatgattg cttatctcaa ctcggaagaa
      301 cgcaatcatc ataatttttc agaacttttg cttttttctt gcctgtctat ttttgccgca
      361 tgcaaaggtt tcattacact attaactaac ggtgtgctat ccgtttccgg gaaagtgaga
      421 aatattgtca acatgaagct ggcgcaccca tggaagctga aagatatttg tgactgcctg
      481 tacatcagtg aaagcctgtt gaagaaaaaa ctaaagcaag agcaaacgac attctcacag
      541 attcttttag atgccagaat gcagcacgca aaaaatttga tacgcgtaga aggttcagtc
      601 aataaaattg ccgaacaatg tggttatgcg agtacatctt attttattta tgcgttccgc
      661 aaacatttcg gcaacagtcc aaagagagtt tctaaggagt accgttgtca aaggcacacg
      721 ggtatgaaca cggataacaa gatgagtgct ttagctattt gattatttgc taacgagtag
      781 tcaaccacac acgctgcgta agaattaaat ggggcagcca ttccctgccc cgcgttgttt
      841 ttaggtgata tatttcttga aataaataag tgacagccat cacatattta tgcatttgca
      901 taacccgttg catgattatt tatgatctca attcggcact ttgtcggtaa aatccaataa
      961 tt
//
LOCUS       DADTFG010000367          958 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.369, whole genome shotgun sequence.
ACCESSION   DADTFG010000367 DADTFG010000000
VERSION     DADTFG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.369"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..477)
                     /locus_tag="J1138_004812"
     CDS             complement(<1..477)
                     /locus_tag="J1138_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA8456472.1"
                     /translation="MRTQGLFFRDQARALSKEHEVDVLYCHRGKRFYSNLYTDDGIKT
                     FYWGYRLRIGSILGMISRIFIYCMMFILYEKKRKVDVIHCHSVAFNQDGSAGIAGVIL
                     GKLFSIPVVITEHATVFSNKNYGRFEKRLMRWALSNADMIICVSEGLRDILEEMTLR"
     gene            complement(516..>958)
                     /locus_tag="J1138_004813"
     CDS             complement(516..>958)
                     /locus_tag="J1138_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBA8456473.1"
                     /translation="EKGVILRQYFYKLTLDSCGHNLRVHYGAFFVYPNVKVGNNCTVE
                     EYSIVSLCDLGDDVIIAARVSIMSGGNHHEIDKLDIKFVDSLLPLKKVKIGSNVWIGT
                     HAIVMNDIPDGMVVGAGSVVTKVFQPDSIIAGIPAKVMRKRGKS"
BASE COUNT          325 a          207 c          120 g          306 t
ORIGIN      
        1 tcttagggtc atttcttcta aaatgtcacg caagccttca ctaacacaaa ttatcatatc
       61 agcattagat aaagcccatc gcattaatct tttctcaaac ctaccataat ttttatttga
      121 aaaaaccgtt gcatgttctg ttataacaac aggaatagaa aataatttac ctaaaatcac
      181 accagcaata ccggcactgc catcttgatt gaaagcaaca ctatgacaat gtataacatc
      241 tacttttctt ttcttttcat agaggataaa catcatgcaa tatatgaaga ttctagatat
      301 cattcctaaa atgcttccta tgcgcagtct atatccccag taaaaagttt taatgccatc
      361 atcggtatat aaatttgaat aaaatctttt tcctctatgg caatataaaa catcaacttc
      421 atgctctttt gaaagtgctc tagcttggtc tctaaaaaaa agtccttgtg ttctcatttt
      481 aggattcgga taccaagatg gaataactgt aattttcacg atttccctct ttttctcata
      541 acctttgcag gaatacccgc aataattgaa tcaggttgaa atacttttgt aacaacactc
      601 ccagcaccaa cgaccatacc atctggaata tcattcatga caatagcatg ggttccaatc
      661 caaacattgg aaccaatttt aacttttttt aaaggaagaa gtgagtcaac aaactttata
      721 tccaacttat ctatttcgtg atgattccca ccgctcataa tcgatactct tgctgcgata
      781 ataacatcat cacctagatc acataagctg acaatagagt attcctctac agtgcaatta
      841 ttaccaacct taacgtttgg ataaacaaaa aaagccccat aatgcactct taaattatgc
      901 ccacaggaat ctaaggtgag tttataaaaa tactgcctta gtataactcc tttttctc
//
LOCUS       DADTFG010000368          952 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.370, whole genome shotgun sequence.
ACCESSION   DADTFG010000368 DADTFG010000000
VERSION     DADTFG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.370"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..195
                     /locus_tag="J1138_004814"
     CDS             <1..195
                     /locus_tag="J1138_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate transporter"
                     /protein_id="HBA8456474.1"
                     /translation="FGDSNATRTTDAFLETECVENVATTEIIKATEESNGHRVSLPLS
                     VFNPQDYHPLLITVSGKNVN"
     gene            274..507
                     /locus_tag="J1138_004815"
     CDS             274..507
                     /locus_tag="J1138_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBA8456475.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            607..>952
                     /locus_tag="J1138_004816"
     CDS             607..>952
                     /locus_tag="J1138_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA8456476.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRNGEINGEHVPEIIL
                     LNSHDGTSSYQML"
BASE COUNT          244 a          260 c          232 g          216 t
ORIGIN      
        1 tttggtgaca gtaatgccac gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa
       61 aatgtcgcca caaccgaaat cattaaagca actgaagaaa gtaacggcca tcgtgtcagt
      121 ctgccgttat cggttttcaa tccccaggac taccatccac tgctgattac cgtttccgga
      181 aaaaacgtta actgatccct gaagtcagtt tcatgcttca gcctctgtca caacgcgtgg
      241 cagaggcttt ttatttatca ggagaaaaca gatatgccgg gttacacaga gtatgtcctg
      301 gctgaaggat ctttctcata cgggcaggcg gttgctgtca taaccgctta ccgcaatgtg
      361 tttatccagg atgacccggg aatgcatttc cgtcgggtta tccgcaacgc ggaaggtcag
      421 cgacgctggc gctgccggaa ttctgaaccc gatgccggaa aggtgcttaa tacgcggctc
      481 gccagtgacg gtcttctcag acaataaacg tcattcattt cacccatcag gccgcgtctt
      541 ctccgggaga cgtggctttt tcatttatta caccagtaaa tcttaaccac cgataaggag
      601 caaattatgc gactagccag tcgttttggt tatgcgaacc agatacgccg tgaccgtccg
      661 ctgacacacg aagaactgat gcactatgtg ccggggattt tcggggaaga taaacacacg
      721 tcccgaagcc agaactatac gtacatcccc accatcaccg tactggaaag cctgcagcgg
      781 gaaggcttcc agccattctt cgcctgccag acccgtgtgc gcgacccggg ccgtcgggga
      841 tacacaaaac acatgctgcg cctgcgccgt aacggagaga taaacggaga acatgtccct
      901 gaaattattc tgctcaactc tcatgacggt acctccagct accagatgct gc
//
LOCUS       DADTFG010000369          947 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.371, whole genome shotgun sequence.
ACCESSION   DADTFG010000369 DADTFG010000000
VERSION     DADTFG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.371"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>947
                     /locus_tag="J1138_004817"
     CDS             <1..>947
                     /locus_tag="J1138_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456477.1"
                     /translation="THQIIKQKYFNDIVPMILALDIRNTYRPDNEMAFYYHIHSFLTQ
                     IPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTK
                     LTATINTCKETCKHLLYLSNEDKEKTISDLDKIQVACYYLTILLAFERRTSLTSALAT
                     LYKMLISEREVSEYECHLLYLTNPIDVMNILNKYIYYFPNENSPFYTLKIDSALSWEA
                     IDAIRDYSISDIYLYPEQKTINCVVEIENIVFGGYIYTLNNGVTLQNIENTLKDSSCH
                     YVLNGYTEFVNCLIQLTSGKTESVHRTINKLNYEKLPFG"
BASE COUNT          351 a          170 c          141 g          285 t
ORIGIN      
        1 acacatcaaa taataaaaca aaaatatttt aatgacattg taccaatgat tctcgcactc
       61 gacatcagga acacatatcg cccggataat gagatggcat tttactatca tattcattct
      121 tttctcactc agataccgga taatgaggat gatatatatc atgctgcaag gacatatctg
      181 cgaaattacg ttaagttatg tttatccgga tacacaccag cgaatgcgca tttcaaagat
      241 atctttgatg gcgtatatga attcattcgt aatattcgca aaaacagtac accaggaaaa
      301 acaaaactta ccgcaactat caacacatgc aaagaaacct gtaaacatct gctttattta
      361 agtaatgaag acaaggaaaa aacaatttct gacttagata aaattcaagt tgcatgttat
      421 tatctcacta tattactggc tttcgaaaga cgaacttcat taacaagcgc cctggcaact
      481 ttatataaaa tgctgataag cgaaagagaa gtttcagaat atgaatgcca tttattatat
      541 ttaaccaacc caatagatgt aatgaatata ctgaacaaat acatatatta ctttcctaat
      601 gagaactcac cattttatac actgaaaatt gacagtgcat tatcgtggga ggccattgac
      661 gcaatacgag actatagtat ttctgatatt tatctttatc ctgaacaaaa aacaataaat
      721 tgtgtcgttg agattgaaaa cattgtcttt ggcggttaca tttatacatt gaacaacggc
      781 gtcacattac aaaacataga aaacacttta aaagattctt catgccatta tgtcttaaat
      841 ggctatacag aatttgttaa ctgtttgata caacttactt caggaaagac tgaaagtgtt
      901 catcgcacca tcaataaact gaactatgag aaattacctt ttggatt
//
LOCUS       DADTFG010000370          943 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.372, whole genome shotgun sequence.
ACCESSION   DADTFG010000370 DADTFG010000000
VERSION     DADTFG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.372"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>943
                     /locus_tag="J1138_004818"
     CDS             <1..>943
                     /locus_tag="J1138_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456478.1"
                     /translation="GRRTAGAVRVDFRSFLPRDDNGARISGPAKPDSHGNDKTSRRRI
                     PALARLLLTLIEAAELNRIDPVYPQPGRTKTQALNAVKLVTEMEHFAQGRPLSEIVFF
                     DPWLKQDRLDARMRELENKGKAWPAGRARTFYQILFSEQVTQDEVVFTSKFGETVFRP
                     EKRVSINGEVDGHREKYWVILMYRRNDAGTVVCRDAYAHALFDYACPVPVDSNLERET
                     INSIMTGGKWLQSSGYELSLNKPLFDITVDLDGEKRFVLPDFLLTVKHPGRGRTSELV
                     IETMGYTDDDYVERKANQHKGMSTLGLLLKDPPHWPEP"
BASE COUNT          211 a          233 c          283 g          216 t
ORIGIN      
        1 gggcgccgaa cagccggcgc cgtgcgtgtt gattttcgct cgttcttgcc gcgagacgat
       61 aatggggctc gtatctctgg gccggcgaag ccagacagtc atggcaacga caaaacaagt
      121 cgtcgccgta taccggcgtt ggcgcgcctg ctgctgacct tgatcgaggc cgctgagctt
      181 aatcgcatcg acccggttta ccctcagcca ggacgaacca aaacgcaggc gctcaatgcg
      241 gtgaagttgg ttaccgagat ggagcatttt gcacaggggc gtcctctgag cgagattgta
      301 ttcttcgacc cctggctgaa acaagatcgg ctcgacgcac gcatgcgcga acttgaaaac
      361 aagggtaaag cctggcctgc agggagagca cgaactttct accagatcct gttctctgag
      421 caggtgacac aggatgaggt cgttttcaca agtaagttcg gtgagacggt atttcgtcct
      481 gaaaagcgag tgagcatcaa cggcgaggtt gatggtcatc gggagaaata ctgggtgatc
      541 ctgatgtacc ggcgcaatga tgctggtacg gtcgtttgcc gcgatgctta tgcgcatgcg
      601 ctctttgatt acgcctgccc ggttccggtc gacagtaacc ttgaacgtga aaccattaac
      661 tccatcatga cgggggggaa gtggctgcag agttcgggct atgagttatc ccttaacaaa
      721 ccgctgttcg atatcactgt ggaccttgat ggcgagaagc ggttcgtctt gcctgacttc
      781 cttttgactg tcaaacaccc tgggcggggg cgaacgtcgg agcttgttat cgagacgatg
      841 gggtatactg atgatgacta tgtcgagcgc aaagcgaatc agcataaagg gatgagtaca
      901 ttgggtttgt tgctgaaaga tccccctcat tggccagagc ctg
//
LOCUS       DADTFG010000371          936 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.373, whole genome shotgun sequence.
ACCESSION   DADTFG010000371 DADTFG010000000
VERSION     DADTFG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.373"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            55..840
                     /locus_tag="J1138_004819"
     CDS             55..840
                     /locus_tag="J1138_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456479.1"
                     /translation="MNLKKVCCICVLFSLLAGCASESPIDEKKKKTQTTQISINKNVP
                     QQLTDKDLYGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVATFSGIPDRKKTVTCNKDKNKDESLDIVDAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWTDYKNQKLTDTMSLRYLVRFTLVDVATGEWATWSPVNYE
                     SKVIFPQIGNKNSDSLDVAEQQISQLKQKTYAAVVKDLVNRYQ"
BASE COUNT          308 a          159 c          205 g          264 t
ORIGIN      
        1 aaaatcgtta gctaaacatt accatgatta tgacaggcga gggagtgtcc gattatgaat
       61 ttaaaaaagg tttgttgtat atgtgtatta ttctcgctgc ttgcaggatg tgcctctgaa
      121 tctcctattg atgaaaaaaa gaaaaaaact caaaccacac aaatttctat taataaaaac
      181 gttccccagc aactaacaga caaagattta tatggtaatg aaaccacact ggctgtatcc
      241 gaggaggata ttcaagctgc gcttgaagga gatgagtttc gtgtgcccct caattctcct
      301 gtaatacttg ttcagtctgg cagtcgcgca ccggaaacca ttatgcagga ggagatgaga
      361 aaatattata cagttgccac attttctggc attcctgaca ggaaaaaaac tgtaacctgt
      421 aataaagaca aaaacaaaga tgaaagcctg gatattgtag atgccgaaaa tatgaactgg
      481 atgcaggcat tgcgttttgt tgcagcaaaa ggccatcaga aagcgattat tgttttccag
      541 gatacgttgc agacaggaaa atatgactct ggattgaaat caatagtttg gacagattat
      601 aaaaatcaaa aattgactga cacgatgtca ctgcgttatt tggttcgatt cacgttggtg
      661 gatgtggcta caggtgagtg ggctacctgg tcgcctgtga attatgaatc gaaagtgatt
      721 tttccgcaga tagggaataa aaattctgac agccttgatg tggcagaaca gcagatttca
      781 caacttaagc agaaaacgta tgcagcagtg gttaaagacc tggtgaatcg atatcagtag
      841 tgaggtttta taccagacgt tttagtggta cttagcaaga aagagcattt ctcttttcta
      901 ttttacacta tggaacaagt ctgctaatta atttta
//
LOCUS       DADTFG010000372          920 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.374, whole genome shotgun sequence.
ACCESSION   DADTFG010000372 DADTFG010000000
VERSION     DADTFG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.374"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(147..710)
                     /locus_tag="J1138_004820"
     CDS             complement(147..710)
                     /locus_tag="J1138_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8456480.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          286 a          171 c          176 g          287 t
ORIGIN      
        1 attaatcatt tcaaatcatg gggttatttt actttagctg gaaatatcaa catgttatgt
       61 gttttattta attgaatggg ctgttttttt atggaagaat tgaggaaaac gtaggaatct
      121 gacattttga atcagaaggt actgttttaa cgaggctttt ttttccacac ccctttaaaa
      181 cgagcagcat tactggctgt gtatctgaca gtatgctgaa tatttcgatg acccagataa
      241 tcctgaagta gtcgggtatc gacaccattg tcagccagag caaaaccaca ggcatgacgt
      301 aacatatgag gatgtgcttt tacagctaat cctgcacgtt ttccagcctc acgaatgata
      361 gaaaaaaaat gttgtctgct gatagggcgg cgtttccggg agagaaaaag ccagtctcct
      421 tcaactccgt tttcaattaa cttcctctgc ttaagccaca acttgatcaa attatattca
      481 tcaggaagaa gaggatgtgt tgtcgagaaa ccatttttaa tacgccgaat attaagttgt
      541 tttccagatg catcaatatc ggataaacgt aaatcaagaa gttcactggc cctaaaaccg
      601 tgaatgaaag ccatcataat cagacagcga tttctttcag gaaaggacat gctctgggct
      661 gcatccatca gcctgtagac ttcatcctgt gtgagatatt ttctcgtcat acgccaccgt
      721 gtacagttgt aaaaaaaaga gaggcacacc cagataagta tgcctgcaat cagaacgtta
      781 tcgatgtaat ctcattatct cattaataag attttttgaa tagtagttct acgcaacaga
      841 ttaaatatga actatttatt gtgcagcttt tctacaagtc atttttaatg cgtgttttta
      901 tctactataa tgtgaaaaaa
//
LOCUS       DADTFG010000373          895 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.375, whole genome shotgun sequence.
ACCESSION   DADTFG010000373 DADTFG010000000
VERSION     DADTFG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.375"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>895
                     /gene="feoB"
                     /locus_tag="J1138_004821"
     CDS             <1..>895
                     /gene="feoB"
                     /locus_tag="J1138_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBA8456481.1"
                     /translation="LMQALGLPGKSFVPLIVGFGCNVPSIMGARTLDAQRERLITIMM
                     APFMSCGARLAIFAVFAAAFFGQDGAGVVFSLYMLGIAVAILTGLVLKYTLMRGEASP
                     FVMELPVYHVPHLKSLLLQTWQRLKGFVLRAGKVIVVASMFIGGLNSFSFSGKTVDNI
                     NDSALASVSKVLTPLLQPMGVHSDNWQATVGLVTGAMAKEVVVGTLNTLYTAEHINKE
                     AFDAANFNLLDELGGALQETWDGLKNTFSLSVLSNPIEASKGDGEMGVGSMGVMSSKF
                     GSGISAYSYLIFVLLYVPCVSV"
BASE COUNT          154 a          285 c          284 g          172 t
ORIGIN      
        1 cctgatgcag gcgctcgggc tgccgggcaa atccttcgtg ccgctgatcg tcggcttcgg
       61 ctgcaacgtg ccgtccatca tgggcgcccg tacgctggac gcccagcgcg aacgcctgat
      121 caccatcatg atggcgccgt tcatgtcctg cggcgcccgt ctggcgatct tcgcggtgtt
      181 cgccgccgcg ttcttcggcc aggacggcgc cggcgtggtg ttctcgctgt atatgctcgg
      241 catcgcggtg gcgatcctga ccggcctggt gctgaaatac accctcatgc gcggcgaagc
      301 ctcgccgttc gtgatggagc tgccggtcta ccacgtgcca cacctgaaaa gcctgttgct
      361 gcaaacctgg cagcggctga aaggcttcgt gttgcgcgcc ggcaaggtga tagtggtcgc
      421 cagcatgttc atcggcggcc tgaacagctt ctcgttcagc ggcaaaacgg tcgacaacat
      481 caacgactcc gcgctggctt cggtctccaa ggtgctgacg ccgctgctgc agcctatggg
      541 cgtccacagc gataactggc aggccaccgt cggcctggtg accggcgcga tggcgaaaga
      601 agtggtggtc ggcacgctga acacgctgta caccgccgag cacatcaata aagaggcgtt
      661 cgacgccgcc aacttcaacc tgctcgacga gctgggcggc gcgctgcagg aaacctggga
      721 cgggctgaaa aacaccttca gcctgagcgt gctctccaac ccgatcgaag ccagcaaggg
      781 cgacggcgag atgggcgtcg gttcgatggg cgtgatgagc agcaaattcg gctccggcat
      841 ctccgcctac agctacctga tcttcgtgtt gctgtacgtg ccttgcgtct cggtg
//
LOCUS       DADTFG010000374          856 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.376, whole genome shotgun sequence.
ACCESSION   DADTFG010000374 DADTFG010000000
VERSION     DADTFG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.376"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>856
                     /locus_tag="J1138_004822"
     CDS             <1..>856
                     /locus_tag="J1138_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456482.1"
                     /translation="NGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVPGVTDP
                     EEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLS
                     TGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRQAL
                     QKGTGAGNISALNKVMKGSLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSC
                     VAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKIL
                     AMIQEQMTW"
BASE COUNT          186 a          216 c          263 g          191 t
ORIGIN      
        1 aacggacagt atcgttacat gggaaatcat ggtcccatgc aactggaagt cccccgtgat
       61 cagtatgccg gcgctgtgga aaccatgaag aataagatcc gcgaaggtaa agtacccggt
      121 gtaaccgatc ccgaagaagc gtcccggctg attcgtcggg gacatctgac ttatacccag
      181 gcccgtaata tcacccggtt cgggaccatc gaatcggtca cttatgatat tgccgagggg
      241 tcggttgtca gtctggcggc cggagggatc agttttgccc tgacggcatc ggtcttctgg
      301 ctcagcaccg gcgatcgcga tgctgccctg cagacagctg ctgtccaggc aggaaaaacc
      361 ttcacccgca cactggctgt ctacgtcaca acccagcaac ttcaccggct cagtgttgtt
      421 cagggtatgc tgaagcatat tgatttttcg acggccagcc cgactgtccg gcaggcgctt
      481 cagaagggga ccggtgcagg aaatatcagt gccctgaaca aagtgatgaa gggttcgctg
      541 gtgacatctc tggcactggt agctgtcaca accggccctg acatgatcaa aatgttgcgg
      601 ggacggatct ccggtgcgca gttcatcagg aatcttgccg tggcatcttc ctgtgtggca
      661 ggtggtgctg tcgggtcagt ggcgggcggg atactgttca gtccactggg accatttggt
      721 gcactgacag ggcgtgtggt tggcggtgtt ctggggggaa tgattgcctc cgctgtatca
      781 ggaaaaattg ccggagcgct ggttgaagaa gatcgcgtca aaattctggc aatgattcag
      841 gagcagatga catggc
//
LOCUS       DADTFG010000375          842 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.377, whole genome shotgun sequence.
ACCESSION   DADTFG010000375 DADTFG010000000
VERSION     DADTFG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.377"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..315)
                     /gene="gyrB"
                     /locus_tag="J1138_004823"
     CDS             complement(<1..315)
                     /gene="gyrB"
                     /locus_tag="J1138_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HBA8456483.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCSDIQVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFD"
     gene            complement(334..>842)
                     /gene="recF"
                     /locus_tag="J1138_004824"
     CDS             complement(334..>842)
                     /gene="recF"
                     /locus_tag="J1138_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004875688.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HBA8456484.1"
                     /translation="EYSDAIAADITATCAQFLPEFALSFSFQRGWDKESDYGELLERQ
                     FERDRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGR
                     RCLYLIDDFASELDTGRRRLLADRLKATQAQVFVSAVSAEQVTDMAGEKGKMFRVEQG
                     KIEVQPQD"
BASE COUNT          167 a          267 c          230 g          178 t
ORIGIN      
        1 gtcgaattta ccgccggcgt gcagcacggt catgatgacc tctgcggctg aaaccccttc
       61 ttccgggtga atgccggtcg gaatgccgcg gccgtcatcc tgcaccgata ccgagttgtc
      121 ggcatggatg gtgacctgaa tgtcactaca gtggcccgcg agcgcttcgt cgatagcgtt
      181 gtccacaacc tcgaatacca tgtggtgcag accggtgccg tcatcggtat cgccgatata
      241 catgcccggg cgcttgcgca ccgcatccag cccttttaat accttgatac ttgaggagtc
      301 ataagaattc gacatcaacg tttctcgctc attttagtcc tgtggttgaa cctctatttt
      361 accctgttcc acgcggaaca tcttgccctt ttcaccggcc atgtcggtca cttgttcagc
      421 gctgaccgcg ctgacaaaaa cctgggcctg ggtggctttc aggcgatcgg ccagcaaccg
      481 gcgacggccg gtgtccagct cggaggcaaa atcatcaatc agatacaggc aacgccgccc
      541 gctctgccgg gtgagaaact caccctgcgc caagcgcaac gcgcacatca gcagcttaag
      601 ctgaccgcgc gataacaaat cctctaccgg cgtgccgtcg gcacgaatgc gaaagtccgc
      661 tttatgcggc cccaccgcgg tataggtcaa cgccctgtcg cgctcaaact ggcgctccag
      721 cagctcgccg tagtcgctct ctttgtccca gccgcgctgg aaggagaagc tcagggcgaa
      781 ttcaggcaga aactgcgcgc aggtcgcggt gatatccgcg gcgatcgcgt cgctgtattc
      841 cg
//
LOCUS       DADTFG010000376          824 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.378, whole genome shotgun sequence.
ACCESSION   DADTFG010000376 DADTFG010000000
VERSION     DADTFG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.378"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..358)
                     /locus_tag="J1138_004825"
     CDS             complement(<1..358)
                     /locus_tag="J1138_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBA8456485.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVSFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKNATLTIWQGVIPLRYVTGVVAGFGMQENNGWQMRYHLRIQPPLWRC
                     GLRQNFRIFQQQDIRTI"
     gene            complement(377..661)
                     /locus_tag="J1138_004826"
     CDS             complement(377..661)
                     /locus_tag="J1138_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005420470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HBA8456486.1"
                     /translation="MPLAAKLTDKGTRHDGYYETVITAGSSTVFIDGLPAARQGDPLT
                     PHAKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNIG"
BASE COUNT          197 a          224 c          205 g          198 t
ORIGIN      
        1 atatcgtccg gatatcctgc tgctggaaga tgcggaagtt ctgccgcagc ccgcaccgcc
       61 ataacggcgg ctggatacgc aggtggtaac gcatctgcca gccgttgttc tcctgcatac
      121 caaaccctgc caccacgcct gtgacataac gcagcgggat gacaccctgc cagatagtca
      181 gcgttgcgtt cttctccagc agcatctccg ccgtctgcat aaaagaatcg ctggcgacat
      241 ccacactcat cacaaacgga taagactggt tctggattaa ccggaagctc accactgcaa
      301 aggtgtccgg ctcctggccg tcaacctcca gcgtaaagcg aagacctttt aaggacataa
      361 atacctctca gacatatcag cctatattta ccgtgccgcc accaataacc acgccgccgc
      421 agtcaatagc atcaccggtt ctggcggcgg gcaggccgtc aataaagacg gttgacgagc
      481 ctctggcgat ttttcgggga tgaggtggat gcttcggttt agcatgcggc gtcagcggat
      541 ctccctggcg ggctgcgggg agaccatcga taaaaacggt ggatgacccg gcggtgatga
      601 cggtctcgta ataaccgtcg tgccgggtgc ctttgtcggt aagtttggct gctaaaggca
      661 taattcctcc tgttcagaat ttctacagca gcaggtttca aaaatccgga ggaatattca
      721 acgaaatatt aatcacttca ccggatattc tgatacgtac cttcactggt ctatttaagt
      781 aatacaaatt gttggcgtgt attaagtttc attttgttct cctt
//
LOCUS       DADTFG010000377          820 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.379, whole genome shotgun sequence.
ACCESSION   DADTFG010000377 DADTFG010000000
VERSION     DADTFG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.379"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            4..>820
                     /locus_tag="J1138_004827"
     CDS             4..>820
                     /locus_tag="J1138_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBA8456487.1"
                     /translation="MALSILLIPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTN
                     DLYPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLT
                     GLNLAFGSFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQ
                     WQNYGAANENEWDGYRFKIKYSIPLTNLFGGRLVYNSFTNFDFGSDLADKSHNNKRTS
                     NAIASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTY"
BASE COUNT          251 a          155 c          173 g          241 t
ORIGIN      
        1 tggatagctt tatccattct attaatacct tgcattggaa acgctcagga aattaaaatt
       61 gatgaaagct ggttacatca aagcttgaat gtcattggtc gcacagactc tcgctttggc
      121 ccaagattga ctaacgacct ctaccctgaa tatactgtag caggaagaaa agactggttt
      181 gatttttatg gttatgttga tctaccgaaa ttctttggcg tcggcagtca ctatgatgtt
      241 gggatctggg atgagggctc accactattt acggaaatag aacctcggtt ttccattgac
      301 aaattgaccg gattaaatct tgcgttcggc tcatttaaag aatggttcat tgcaaacaac
      361 tatgtctatg atatgggtga caaccagtca tcccggcaaa gtacatggta tatggggctt
      421 ggtacagata tcgacacggg tctaccaatt aagctttctg ccaatatata cgccaagtat
      481 cagtggcaaa actatggtgc cgctaatgaa aatgaatggg acggatatcg attcaaaata
      541 aaatatagca tccctcttac aaatttattc ggagggcgat tggtatacaa tagttttact
      601 aactttgatt ttggctccga tcttgcggac aagtcacaca ataataaacg aaccagtaat
      661 gctattgctt caagccatat cctttccctt ctatatgaac actggaaatt tgcatttaca
      721 ctacgttatt ttcacaacgg tggacaatgg aatgcgggag agaaggttaa cttcggagat
      781 ggtccatttg aattaaaaaa tacaggatgg ggaacctata
//
LOCUS       DADTFG010000378          819 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.380, whole genome shotgun sequence.
ACCESSION   DADTFG010000378 DADTFG010000000
VERSION     DADTFG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.380"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            150..>819
                     /locus_tag="J1138_004828"
     CDS             150..>819
                     /locus_tag="J1138_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA8456488.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALE"
BASE COUNT          273 a          129 c          172 g          245 t
ORIGIN      
        1 ttttttataa aattaaatat aaaattatgg atttagaata tttttatttc taatagacga
       61 gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt taccagtaat
      121 gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat attgttgcta
      181 aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt gtagaaatct
      241 atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta aaattcgtta
      301 ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca attgataaag
      361 ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt ggtcctaatg
      421 gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt gctaacgttc
      481 gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtt agctatatgt
      541 ttgacaatac gggtgtgatt gtatttaaag ggacagaccc tgaccatatt tttgaaattt
      601 tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat attgttattt
      661 atactgaacc tactgacctt cataaaggaa tcgcggcgct aaaagcggct ggaatcactg
      721 agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt tccccagaag
      781 atttagaaat ctttgaaggg cttgttgatg cccttgaag
//
LOCUS       DADTFG010000379          803 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.381, whole genome shotgun sequence.
ACCESSION   DADTFG010000379 DADTFG010000000
VERSION     DADTFG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.381"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>803
                     /locus_tag="J1138_004829"
     CDS             <1..>803
                     /locus_tag="J1138_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456489.1"
                     /translation="EGYIISEADYATNVVFFNDVLDKYIDAFNRSKTADIVAAEVDQE
                     RLRNTDTRLTNELPGALSEDVLLKYFTFTQNSECDRNWLVRYLPVGVSKEYVARELNQ
                     NVYGDFPSVSEVKRNILHRLHYVLWQSQAKLTIEVNNLETLLMEVAQRSADQNNYILV
                     IYGSRFSEELRELIYQPERHDAFSIHVDVHARGISSLPFRINNCLISLILNSEKEFSL
                     MVSAESFGELRLFRYPDGTLFNTFYRSSDDPLEGVMKTLWEMEMEITDT"
BASE COUNT          228 a          164 c          183 g          228 t
ORIGIN      
        1 tgaaggctat atcatttcag aagcggatta tgcaacgaat gtggtgttct ttaatgatgt
       61 tctggataaa tacatagatg ccttcaaccg tagtaaaacg gcagatatcg ttgctgctga
      121 agtagaccag gagcgactta gaaatactga tactcgtctt acaaatgaac ttcctggggc
      181 attgtctgaa gatgttctgc ttaaatactt cacgtttact caaaattcag aatgtgatcg
      241 caattggctg gtgagatacc ttcctgttgg tgtttccaaa gagtatgttg ccagagaact
      301 taatcagaat gtctacggag atttcccttc ggtatcagaa gtgaagagaa atattctcca
      361 cagactgcat tatgtactgt ggcagtcaca ggctaaatta acgattgagg tcaataacct
      421 cgaaacgctg ttaatggaag tcgcgcaacg gtctgctgat cagaataact atattctggt
      481 gatctatggt tcccgcttca gcgaagaact gagggaactg atctaccagc cggaaaggca
      541 tgatgcgttt agcatacatg ttgatgtgca cgccagaggt atcagttctc tgcccttcag
      601 gatcaataac tgtcttattt cccttatcct caattccgaa aaggaatttt cgctcatggt
      661 cagtgctgaa tcctttggtg aactcaggct gttcaggtat ccggatggaa cccttttcaa
      721 taccttctac cgcagcagcg acgatccact tgaaggtgta atgaaaacgc tctgggaaat
      781 ggagatggaa atcactgata cac
//
LOCUS       DADTFG010000380          801 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.382, whole genome shotgun sequence.
ACCESSION   DADTFG010000380 DADTFG010000000
VERSION     DADTFG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.382"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..771
                     /gene="mazG"
                     /locus_tag="J1138_004830"
     CDS             <1..771
                     /gene="mazG"
                     /locus_tag="J1138_004830"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBA8456490.1"
                     /translation="RLLNIMKTLRDPQNGCSWDRKQTFATIAPYTLEETYEVLDAIER
                     QDYADLRDELGDLLFQVVFYAQMGQEQGLFDFDEVCTAISDKLERRHPHIFGDAEGGD
                     SEAVAARWEQLKAGERAEKALHSVLDDIPQALPALMKAHKIQKRCASVGFDWNTLGPV
                     LDKVYEEIDEVMHEARQAVVDESKLEEEIGDLLFATVNLSRHLGHKAENALQAANRKF
                     ERRFREVEAIVKQRGLRLEDATLEQMEEAWQQVKKSEI"
BASE COUNT          180 a          223 c          269 g          129 t
ORIGIN      
        1 cgcctgctga acatcatgaa aacgctgcgc gacccgcaga acggttgctc atgggaccgt
       61 aagcaaacct tcgccaccat cgcgccctac acgctggaag agacttacga agtgctggac
      121 gccatcgaac ggcaggacta cgccgatctg cgcgacgaac tgggcgactt gctgtttcag
      181 gtagtgttct acgctcagat ggggcaggaa caggggctgt tcgattttga tgaggtgtgt
      241 actgccatca gcgacaagct ggagcgccgt catccgcaca tcttcggcga cgccgagggc
      301 ggagacagcg aagccgtggc ggcgcgctgg gagcagctca aggccggcga gcgcgccgaa
      361 aaagcgctgc actcggtgct ggacgacatt ccgcaggcgt tgccggcgtt gatgaaggcg
      421 cacaagatcc aaaaacgctg cgcctcggtc ggcttcgact ggaacacgct gggcccggtg
      481 ctggacaaag tgtatgaaga gatcgacgaa gtgatgcacg aagcccgcca ggcggtggtc
      541 gatgagagca agctggaaga ggagatcggc gatctgctgt tcgccacggt gaacctctcg
      601 cgccatttgg gccacaaggc cgagaacgca ttgcaggcgg ccaaccgcaa gttcgaacgc
      661 cgcttccgcg aggtggaggc gatcgtcaag caacgcgggc tacgcttgga agacgccacg
      721 ctggagcaga tggaagaagc ctggcaacag gtgaagaaat ctgaaatctg atgcgcttcc
      781 ggcgttcatg gcgccggggt g
//
LOCUS       DADTFG010000381          796 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.383, whole genome shotgun sequence.
ACCESSION   DADTFG010000381 DADTFG010000000
VERSION     DADTFG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.383"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            95..724
                     /locus_tag="J1138_004831"
     CDS             95..724
                     /locus_tag="J1138_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBA8456491.1"
                     /translation="MKEFSEIAKSAHISNDAIVEYPIHCGPNSQIHGGCNVGQFTFIN
                     ISSVLYPNVKIGRFCSIARNCEIGVARHPVNFLSTHSFQYHFAQFPKHPFYKNGINRT
                     SWRAHPDTIIGSDVWIGAQSIVKAGVNIGHGAIIAANSVVTKNIAPYSIVGGSPAKVI
                     RMRFNAEQISKLLELRWWELSLEEISDLEFGNIDSCIESLLKIRRIEHL"
BASE COUNT          268 a          123 c          151 g          254 t
ORIGIN      
        1 ataaacatcc ctatctcttt tttaaagatg caatcaaaaa tattggggta aaaacaaaga
       61 aaataataat taatgccaaa ggaagtcaga tacaatgaaa gaattttcag aaatagcaaa
      121 aagtgctcat atatcaaatg atgccatcgt tgagtatccg atacattgtg gcccaaactc
      181 tcaaatacat ggaggatgta atgtcggcca attcacattt ataaatatat caagtgtctt
      241 atatccgaat gttaaaatag gtcgtttctg ctctattgca agaaattgtg aaattggtgt
      301 tgcccgtcat ccagtcaatt ttttgtctac acatagtttc cagtatcact ttgcacagtt
      361 tcccaaacat cctttttata aaaatggtat aaacaggaca tcctggagag cacatccaga
      421 tactattatt ggtagtgatg tttggattgg tgctcaatcg attgttaaag ccggtgttaa
      481 tatcggtcat ggtgcaataa ttgcagcaaa ttcggtggtt actaaaaata ttgcaccgta
      541 ttcaatagtc ggaggaagtc ctgcaaaggt aataaggatg cgttttaatg cagagcaaat
      601 ttctaaactc ttagaactac gttggtggga gttatcatta gaagagattt cggatctgga
      661 gtttggaaat attgattctt gtattgaatc attgttaaaa ataaggcgta ttgagcattt
      721 gtaaaaaaca aacttcaatg taactctatg tttttgacgt aagtatagga acacctcttg
      781 tatttcaact tggatg
//
LOCUS       DADTFG010000382          772 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.385, whole genome shotgun sequence.
ACCESSION   DADTFG010000382 DADTFG010000000
VERSION     DADTFG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.385"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..195
                     /locus_tag="J1138_004832"
     CDS             <1..195
                     /locus_tag="J1138_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456492.1"
                     /translation="FGDSNATRTTDAFLETECVENVATTEIIKATEESNGHRVSLPLS
                     VFDPQDYHPLLITVSGKNVN"
     gene            296..427
                     /locus_tag="J1138_004833"
     CDS             296..427
                     /locus_tag="J1138_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBA8456493.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            606..>772
                     /locus_tag="J1138_004834"
     CDS             606..>772
                     /locus_tag="J1138_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA8456494.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLE"
BASE COUNT          204 a          200 c          189 g          179 t
ORIGIN      
        1 tttggtgaca gtaatgccac gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa
       61 aatgtcgcca caaccgaaat cattaaagca actgaagaaa gtaacggcca tcgtgtcagt
      121 ctgccgttat cggttttcga tccccaggac taccatccac tgctgattac cgtttccgga
      181 aaaaatgtta actgatccct gaagtcagtt tcatgcttca gcctctgcca caacgtatgg
      241 cagaggctat ttatcaggag aaaccagata tgccgggtta cacacagagt atgtcctggc
      301 tgaaggatct ttctcatacg ggcaggcggt tgctgtcata accgcttacc gcaatgtgtt
      361 tatccaggat gacccgggaa tgcatttccg tcgggttatc cgcaacgcgg aaggtcagcg
      421 acgctgacgc agccggaact ctgaagctga tgccggaaaa cagcttaatg cgtggctcgc
      481 cagtggaggg cttctcagac aataaacctc attgatttca tccatcaggc cgcgtcttct
      541 ccgggagacg cggctttttt catttattgc accagtaaat cttaaccacc gataaggagc
      601 aaagtatgcg attagccagt cgttttggtt atgctgcaaa ccagatacgc cgtgaccgtc
      661 cgctgacaca tgaagagctg atgcaccatg tgccggggat tttcggggaa gataaacaca
      721 cgtcccgaag ccagaactat acgtacatcc ccaccatcac cgtactggaa ag
//
LOCUS       DADTFG010000383          769 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.386, whole genome shotgun sequence.
ACCESSION   DADTFG010000383 DADTFG010000000
VERSION     DADTFG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.386"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..696
                     /gene="galE"
                     /locus_tag="J1138_004835"
     CDS             <1..696
                     /gene="galE"
                     /locus_tag="J1138_004835"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBA8456495.1"
                     /translation="NCAIKNDVANFIFSSSATVYGESEIMPVTEDCHIGGTLNPYGTS
                     KYISELMIRDIAKKYSDTNFLCLRYFNPTGAHESGIIGENPADIPSNLVPYILQVAMG
                     KLEKLMVFGGDYPTKDGTGVRDYIHVMDLAEGHVAALSYLSSCNNTNYHVFNLGTGKG
                     YSVLELVSTFEKISGVGIPYEIVSRREGDVAESWSSPEKANKYLNWSAKRALETMLED
                     AWRWQLKNPNGYK"
BASE COUNT          267 a          108 c          164 g          230 t
ORIGIN      
        1 aattgcgcta taaaaaacga tgtggctaat tttattttta gctcttctgc aacggtttat
       61 ggcgaaagcg aaataatgcc tgttacagaa gattgccata taggcggaac attaaaccca
      121 tatggcacat cgaagtatat atcagaactg atgattagag atattgcaaa aaaatatagt
      181 gacactaatt ttttatgtct gagatatttt aacccaacag gtgctcacga gtcgggaata
      241 atcggggaaa atcctgcaga tataccaagc aatttagttc catatatatt acaagttgcg
      301 atgggtaagc tagaaaaact tatggtattt gggggggatt atcctacaaa ggatggaacc
      361 ggtgttcgtg attatataca cgtaatggat ttagcagaag ggcatgtggc tgctttatct
      421 tacctttcca gttgcaataa cactaattat catgttttta atttaggtac tggtaaagga
      481 tattctgttt tagaattggt ttctaccttt gaaaaaatat ctggggttgg aattccgtat
      541 gaaattgttt cgagaagaga aggggatgtt gctgaaagtt ggtcatcacc cgaaaaagca
      601 aataagtatc tcaattggag tgctaaaagg gcgttggaaa caatgcttga ggatgcctgg
      661 cgctggcaat tgaagaatcc aaatggttat aaataataac actgtgaaaa aatagctaaa
      721 gcatataatt ataatatgta agctaacaca taatttaaaa aaatgtaaa
//
LOCUS       DADTFG010000384          769 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.387, whole genome shotgun sequence.
ACCESSION   DADTFG010000384 DADTFG010000000
VERSION     DADTFG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.387"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..464
                     /locus_tag="J1138_004836"
     CDS             <1..464
                     /locus_tag="J1138_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HBA8456496.1"
                     /translation="AAQKLRGERQFCRHISVFVATSPFAAGETYYGNQATTRLATPTQ
                     DSRDIIQAARRALERIFIAGHRYHRCGVMLGDFTSERVAQFGLFDDAPPRKNSDRLMQ
                     LLDSLNQDGRAPVWFAGQGIPDRADGWKMKRQRLSPAWTTRLQDVPAVRLK"
BASE COUNT          151 a          240 c          242 g          136 t
ORIGIN      
        1 gcgcggcaca aaagctgcgt ggcgagcggc agttctgccg gcacatctcg gtgttcgtgg
       61 ccaccagtcc gtttgccgcc ggcgagacct attacggcaa ccaggcgacc acccgactag
      121 caacgcccac ccaggacagc cgtgacatca ttcaggccgc ccggcgggcg ctcgaacgga
      181 tcttcatcgc tggccaccgc tatcaccgtt gcggcgtgat gctgggcgat ttcaccagcg
      241 aacgtgtggc gcagttcggc ctgtttgacg acgcgccgcc gcgcaaaaac agcgatcggc
      301 tgatgcagtt gctcgacagc ctcaatcagg acggccgggc gcctgtgtgg tttgccggac
      361 agggtattcc cgatcgggcc gacggctgga agatgaaacg acagcggctg tcgccggcct
      421 ggactacacg gctgcaggat gtcccagctg tgcgtctgaa gtgaatgcgc gtcagcgcat
      481 ggaggcgaca gctgagctgc atgagaacgc catcaggtca gggtcgccgg agacgcggca
      541 aagccgccca ggcctgatag gtatcgccta tcccatccct cgccctgcaa gaccagtccc
      601 gctaaatgcc atgtatatct ttgtgtgcgc cgtcggagtg gtcacggcaa ggcgtagccg
      661 cagtcggggc gtatctccgc gttagcgggc cgtgagggcc gcttacgggc gtgtacgata
      721 gaacttacag cgatgctact caaagcaact caactcctag ttacaacat
//
LOCUS       DADTFG010000385          766 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.388, whole genome shotgun sequence.
ACCESSION   DADTFG010000385 DADTFG010000000
VERSION     DADTFG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.388"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..265
                     /locus_tag="J1138_004837"
     CDS             <1..265
                     /locus_tag="J1138_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBA8456497.1"
                     /translation="RGCLFNSFKFWFNDNICISAIVTSVIFSALHLQYTDFRTFLMLF
                     LVSLVLISAKIKSNGLLMPILLHMSMNAVIAGIQYLAYISYTH"
     gene            complement(440..718)
                     /gene="mcmA"
                     /locus_tag="J1138_004838"
     CDS             complement(440..718)
                     /gene="mcmA"
                     /locus_tag="J1138_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin McmA"
                     /protein_id="HBA8456498.1"
                     /translation="MRKLSENEIKQISGGDGNDGQAELIAIGSLAGTFISPGFGSIVG
                     AYIGDKVHSLATTATVSPSMSPSGIGLSSQFGSGRGTSSASSSAGSGS"
BASE COUNT          232 a          158 c          127 g          249 t
ORIGIN      
        1 tcgagggtgt ctttttaact cttttaaatt ctggtttaat gacaatattt gcatctctgc
       61 tattgtaaca tcagtcatat tttctgcgtt acatctacaa tatactgact ttcgtacatt
      121 tttgatgctc tttctggtat cgttagttct tattagtgca aaaataaagt cgaatggcct
      181 gttaatgccc atattacttc acatgtcgat gaatgcagtc atagcaggaa tacaatatct
      241 ggcttatatc agttatactc attaacagag cattttctgt tacaatctca tatgataaaa
      301 attaaacgga ctggcattgt tactatgcgg tatgcataaa atgataaacg ttcggaggag
      361 cctaacttaa accgttacat aggcaccatt atcatataat gaagcacctg ttacaggtgc
      421 ttcattaaca atataaggtt taacttccac tccccgcaga cgaagaggca cttgatgtac
      481 ctctgccaga tccaaactgg gatgataatc ctatacctga gggagacatg gagggactta
      541 ccgttgcagt cgttgccaat gaatggactt tatcacctat ataagcccct acaatagaac
      601 caaatcccgg actaataaac gtaccagcaa gtgaaccaat agcaattaat tctgcctgcc
      661 cgtcattccc gtcacctcca gatatttgtt ttatttcatt ttcagatagt tttctcattt
      721 caacaccttc gctataagat ttgtgaaaaa gtaaaaattt acgttt
//
LOCUS       DADTFG010000386          762 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.389, whole genome shotgun sequence.
ACCESSION   DADTFG010000386 DADTFG010000000
VERSION     DADTFG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.389"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..>762)
                     /gene="wzy"
                     /locus_tag="J1138_004839"
     CDS             complement(<1..>762)
                     /gene="wzy"
                     /locus_tag="J1138_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O22 family O-antigen polymerase"
                     /protein_id="HBA8456499.1"
                     /translation="VFNGGYEERYFMVLLLMLFVLIVFTVELKDVRLEQCYNGISAAV
                     IVTLLIVLYEIISGNHMSTSRYSDINNEAFLWSVSKPTAFYYNENDMLSFVVCFLPFT
                     FVLKNTMLRVAIFLLAAASAIYIGSKAAIITILIYGLFTKIISKKAILLLVVTGVVLV
                     NLFATSLIENTSSFISGSSERLLGFWDTILSTRGGDESTSERLSIYKANLDWLGNHPL
                     SATFGNGRFGDYESELMKVYGLRMGEFHNLHLEMIT"
BASE COUNT          283 a          151 c           98 g          230 t
ORIGIN      
        1 agttatcatt tcaagatgta aattatgaaa ttcccccatt cttaaaccat aaactttcat
       61 caattcgctt tcataatcac caaaccttcc gttcccaaat gtagctgaca aaggatggtt
      121 accaagccaa tccagattag ctttgtaaat ggaaagacgt tctgaggtac tttcatctcc
      181 accacgagtc gaaagtatcg tatcccaaaa accaagtaat ctttctgaag agccggatat
      241 aaaggaactg gtattttcaa ttagtgatgt tgcaaataaa ttgacaagaa caacaccagt
      301 taccactaaa agtaatatcg cctttttaga gattattttt gtaaataacc cataaattag
      361 aatcgttatt attgcagctt tcgaacctat atatatagca ctagcagcag ccagtaaaaa
      421 tattgcgaca cgcaacatag tgttctttaa tacaaaagta aacggtaaaa agcaaacaac
      481 aaagctcaac atatcatttt cattataata gaaggctgtt ggttttgaaa cactccataa
      541 aaatgcctca ttgtttatat ctgaataacg tgatgtactc atatggttac cactaattat
      601 ttcgtataac acaattagca aggtaacaat tactgcagct gatatcccat tataacactg
      661 ttctaatctt acatctttca attctacagt aaatacaatt agaacaaata gcattaacaa
      721 taaaaccata aaatatcgtt cttcatatcc cccattaaat ac
//
LOCUS       DADTFG010000387          760 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.390, whole genome shotgun sequence.
ACCESSION   DADTFG010000387 DADTFG010000000
VERSION     DADTFG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.390"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            6..635
                     /locus_tag="J1138_004840"
     CDS             6..635
                     /locus_tag="J1138_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8456500.1"
                     /translation="MQNRKFLTHHEINLLLQSVKQKSCSSRDVCMILLAYFHGLRVSE
                     LLSLQLSDLELTTEKIYIQRIKNGFSTVHPLQKEEVIAITNWLNERNSLNVKHFNDNP
                     WLFVSRTGKPLSRQRFYNIVSAAGKNAGLNIKVHPHMLRHACGYSLADNGVDTRLIQD
                     YLGHRNIRHTVIYTASNSMRFEKMWGIGDAKKQHFDPKCKPNLCLEILV"
BASE COUNT          262 a          122 c          134 g          242 t
ORIGIN      
        1 gatttatgca aaacagaaaa tttttaacgc atcatgaaat taatttacta cttcaatctg
       61 taaaacaaaa gagttgctct tcacgcgatg tttgcatgat tttattggct tattttcatg
      121 gtctgcgtgt tagtgagctc ttatctctgc agttgtcgga tttagaacta actacagaaa
      181 aaatatatat tcaacggata aaaaatggat tcagtacagt ccatccactt caaaaggaag
      241 aggtgattgc aataacaaac tggctgaatg aaaggaattc attaaatgtt aaacatttca
      301 atgataaccc gtggttattt gtttcccgaa caggaaaacc attatcgaga caacgatttt
      361 ataacatagt ttctgctgcg ggtaaaaatg caggtttaaa tattaaagtt catccacata
      421 tgttacgcca tgcatgtggt tattcactag cagataatgg tgtggatacc cgtcttattc
      481 aggactatct cgggcatcgt aatatcaggc atacggtaat ttatactgct tctaattcaa
      541 tgagatttga aaaaatgtgg gggataggtg acgcaaaaaa gcaacatttt gacccaaaat
      601 gtaaacccaa tctttgttta gaaattctcg tatagaaaaa catatttttc tataaaaaac
      661 attaaattta ctgatttaga tcaatgtgcg acgtcaatct acacataaag aggtaggttt
      721 gtcttctttt taatctctgg cgtataaagc caaaatgtaa
//
LOCUS       DADTFG010000388          749 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.391, whole genome shotgun sequence.
ACCESSION   DADTFG010000388 DADTFG010000000
VERSION     DADTFG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.391"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            146..742
                     /gene="fimE"
                     /locus_tag="J1138_004841"
     CDS             146..742
                     /gene="fimE"
                     /locus_tag="J1138_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HBA8456501.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          194 a          144 c          185 g          226 t
ORIGIN      
        1 atttttgtat gatatttaat gtaattgact gatagccaca tcacttcggg tgtggttatc
       61 tttttatcta ttgggctaat tttgaccgat tgaggtttcc taaaggtatt cattcaaata
      121 tatctcagtc aggagtacta ctattgtgag taaacgtcgt tatcttaccg gtaaagaagt
      181 tcaggccatg atgcaggcgg tgtgttacgg ggcaacggga gccagagatt attgtcttat
      241 tctgttggca tatcggcatg ggatgcgtat tagtgaactg cttgatctgc attatcagga
      301 tcttgacctt aatgaaggtc gaataaatat tcgccgactg aagaacggat tttctaccgt
      361 tcacccgtta cgttttgatg agcgtgaagc cgtggaacgc tggacccagg aacgtgctaa
      421 ctggaaaggc gctgaccgga ccgacgctat atttatttct cgccgtggga gtcggctttc
      481 tcgccagcag gcctatcgca ttattcgcga tgccggtatt gaagctggaa ccgtaacgca
      541 gactcatcct catatgttaa ggcatgcttg tggttatgaa ctggcggagc gtggtgcaga
      601 tacccgttta attcaggatt atctcgggca tcgaaatatt cgccatactg tgcgttatac
      661 cgccagtaat gctgctcgtt ttgccggatt atgggaaaga aataatctca taaacgaaaa
      721 attaaaaaga gaagaagttt gatttaact
//
LOCUS       DADTFG010000389          740 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.392, whole genome shotgun sequence.
ACCESSION   DADTFG010000389 DADTFG010000000
VERSION     DADTFG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.392"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>740
                     /locus_tag="J1138_004842"
     CDS             <1..>740
                     /locus_tag="J1138_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456502.1"
                     /translation="LIGQDNDFLDHLKIPKQHLLELISRGRIQFALPQNIIRYNLSFL
                     EECLNTNPECIILSRRLASSSIIDIRNKTGFLATTFSFEEKLNVLKTLRALDGKIFNL
                     FIKALSQNWHSMEMNIHERGAMGVSNIGIAPVMSELFNRQGRDLLLELLFSAMPLEWA
                     MALNADFYPYHSNEHSQYNASACCLYGYNGFRINEEAIVKSKIGEIATRILSIDNDMS
                     ALELDDAILKGDIPRIRDFSRSLSNLSS"
BASE COUNT          257 a          130 c          129 g          224 t
ORIGIN      
        1 ctcatcggac aagacaatga cttcttagac catttaaaaa taccgaaaca acatttacta
       61 gagttaatat cgagaggtag aattcaattt gcattaccac aaaacataat aagatataac
      121 ttatcatttc ttgaagaatg cctaaacact aatcctgaat gcattattct gtccagacga
      181 ttagcatcat cctcaattat tgacatcaga aataaaacag gttttcttgc tacaacattt
      241 tctttcgaag aaaaattaaa tgttctcaaa acattgaggg cactggatgg caaaatattc
      301 aacttattta tcaaggccct atctcaaaat tggcactcaa tggagatgaa tatccatgag
      361 cgtggagcca tgggtgtttc aaacattggc atagcccccg tcatgtcaga gttattcaat
      421 aggcaaggac gagaccttct tcttgaacta ttattttctg ctatgccatt ggagtgggcg
      481 atggcattaa atgctgattt ttatccatac cacagcaatg agcattcaca atataatgct
      541 tctgcatgtt gtttatatgg atataatggt tttagaataa atgaagaggc tattgtaaaa
      601 tctaaaatcg gtgaaatagc aacaagaata ctatcaatcg ataatgacat gtcagcctta
      661 gaattagacg acgcaatact taaaggtgat attccgagga taagggattt ttcaagatcg
      721 ttgtctaatt tatcaagtga
//
LOCUS       DADTFG010000390          723 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.393, whole genome shotgun sequence.
ACCESSION   DADTFG010000390 DADTFG010000000
VERSION     DADTFG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.393"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          220 a          123 c          132 g          248 t
ORIGIN      
        1 aaagagttaa ttctgagcat ccatacattt tacctatgtc aggtaaaata aactatgggc
       61 ttgattattc accattattc atgtttctac taagaaaaac aggtgaaaaa tcggatcaag
      121 tttatagtga agcgattcta cgactcgatc tgcctgatcc aaccttcttg ctagttgctc
      181 tacacgaaac agaccaaaga aattttgttc ggtgcggcaa atccagttat tacaatgggc
      241 tgtatgttga tgagaatggt tacttgaaat gttttctcca gatttaaagg catcaggcat
      301 atcaatatta tgtaagtgct gtacttacac tctaaacggg aaattattaa atgaacaagt
      361 tgaaattttt ctgaccttat taacttaaag aatagacgca tcaattgttt tatttcaggg
      421 cgttaaacac attgtattta taattatgca aaatttacca atgttttaac agatccgata
      481 gtagctattg ttgttttcgg agtttggccc tgagctgtcc atgccttttt agaattatca
      541 gtatcaatag atggtaatac ctccaattag ttgaattagg ggtataatag tcgcttttaa
      601 ggttcttcca tggtcacagt taaccttcat tgtccctgtt gtcagcccgt tcaggtttac
      661 cgtccaatag agttatactt agctgaacct agatttttag cttcgggtat gtaaaaatag
      721 gta
//
LOCUS       DADTFG010000391          723 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.394, whole genome shotgun sequence.
ACCESSION   DADTFG010000391 DADTFG010000000
VERSION     DADTFG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.394"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            137..>723
                     /locus_tag="J1138_004843"
     CDS             137..>723
                     /locus_tag="J1138_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA8456503.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADASKAATAGYVKTNSAYTITY"
BASE COUNT          219 a          152 c          145 g          207 t
ORIGIN      
        1 tgaatgaatg taatacttag taaaaaacaa acaaacatca ttaacaaagt aacgttcagt
       61 tacatggcat tgcatcaaaa tctcatcatc atgcaatgct gttatctgct ttatggaata
      121 ttactcaagg atttttatgt ctaaaaaatt aggttttgcg ctgtccggta ttatgttggc
      181 aatggctgct ggtactgcat ttgctgctaa cgatatggac ggcggtcaat taaatattag
      241 cggtctggtc gttgataaca cctgtgaagc tcgtgttgat ggcggtaaca aagacggtct
      301 gatcctgctg caaaccgcta ctgttgctga aattactgat ggtgtactgg ataccactgt
      361 aggcgctaaa gctaaaccat tcagcattac tattgactgc agcaaagcta acccggctcc
      421 gggtactact gcaaaaatga ccttcggttc tgttttcttt ggtaacagca aaggaacact
      481 gaacaacgac atgtctatta acaccccgtc tgacggcgta aatatcgctc tgcacaatat
      541 tgaaggttct actattaaac aggttcaggt caataatccg ggtgatgttt acagcaaaac
      601 tctggattca acgtctaaat ctgcaaccta cgattttaaa gcatcctatg ttcgtgcgga
      661 tgcaagcaaa gcagctactg ccggttatgt aaaaaccaac tcagcatata ccattactta
      721 cca
//
LOCUS       DADTFG010000392          714 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.395, whole genome shotgun sequence.
ACCESSION   DADTFG010000392 DADTFG010000000
VERSION     DADTFG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.395"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>714
                     /locus_tag="J1138_004844"
     CDS             <1..>714
                     /locus_tag="J1138_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBA8456504.1"
                     /translation="DNDGPAYGEFLGLDGAVTNAGWQAMIKSGVRVINNSWGVSIPDF
                     LSDGGRDPNALHFELKDAQEQFDQVKPLLGSLAGAGYQGAIDAARKNILVLFAAGNDG
                     NYNQPDVISGLAYFVPDIAPNWLSVASVAQDAASANSVPYTISSFSSRCGYTASFCVS
                     SPGSKIYSTVANGSDPANLVSDYGNKNGTSMATPHVTGAVAVLLQRFPYMNSAQIADV
                     LKTTATDMGAPGIDALYGWG"
BASE COUNT          136 a          233 c          225 g          120 t
ORIGIN      
        1 cgacaacgac ggcccggcgt acggcgaatt cctcggcctg gacggcgccg tcaccaatgc
       61 cggctggcag gcgatgatca agagcggcgt gcgggtgatc aacaacagct ggggcgtcag
      121 cattcccgat ttcctgtccg acggcggccg cgatcctaac gcgctgcact tcgagctgaa
      181 ggatgcgcag gagcaattcg atcaggtcaa gccgctgctc ggtagcctgg ccggcgcagg
      241 ctatcagggc gccatcgacg cggcgcgcaa aaatattctg gtgctgttcg ccgccggtaa
      301 cgacggcaat tacaaccaac cggatgtgat cagcgggctg gcctacttcg tgccggacat
      361 cgcgccgaac tggctgtcgg tcgccagcgt ggcgcaggat gcggcatcgg ccaacagcgt
      421 gccttacacc atcagcagct tctcgtcccg ctgcggctat accgccagct tctgcgtctc
      481 gtcgccgggc agcaaaatct acagcaccgt ggccaacggt tccgatccgg ccaatctggt
      541 gtcggactac ggcaataaaa acggcacttc gatggcgacg ccgcacgtca ctggcgcagt
      601 ggcagtgttg ctgcagcgct tcccgtacat gaactcggcg cagattgccg acgtgctgaa
      661 aaccaccgcc accgacatgg gcgcgccggg catcgacgcg ctctacggct gggg
//
LOCUS       DADTFG010000393          714 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.397, whole genome shotgun sequence.
ACCESSION   DADTFG010000393 DADTFG010000000
VERSION     DADTFG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.397"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            1..714
                     /locus_tag="J1138_004845"
                     /pseudo
     CDS             1..714
                     /locus_tag="J1138_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          231 a          143 c          119 g          221 t
ORIGIN      
        1 tccttatttc tggaaaccac gcctattaaa gttaaccatt tgcgaagcat actggattat
       61 attgaacagg aacagttaac tgccgaaaaa cgtaaccatt gcatgaaact gtccaaaaaa
      121 aattcataga gaaaaaacca tacagccaac agtaaatctc aatgatagtg catttttttg
      181 caatctcctt ctgacactat tttttgtcgc catctgtcat taaaatacgc ccttgattca
      241 ttgagaaatg gaaaaggcaa agtcaacctg attaaacatt actcctccat tgaatccata
      301 cagcagcatg ttcccttagt ccgggacgcg gaattcagag cattacttcg ccatcctctt
      361 gcaaggagtc gcgttatcgc gagtcaggat tttggctttg ctttagatat tttcttctgt
      421 cgaatgatgg caaacaatgt cagtcatatg tccgcgattt tatatataga caatcatact
      481 ttgtcagtaa gtaaagatgt cgtgttatct tatatatcaa cgttgggtac ttttgaaaaa
      541 aatattcttt tagccaacgt cagttattta cactgttggc cgctaaaaat catgacaaca
      601 tgtcagctgt tcatatagcc attcatcata atcatcataa aactgtagaa acatattatg
      661 ctgctataaa tgtaatcagc caaagcctga gttttagtgc tgatgaacta aaga
//
LOCUS       DADTFG010000394          710 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.398, whole genome shotgun sequence.
ACCESSION   DADTFG010000394 DADTFG010000000
VERSION     DADTFG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.398"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..602)
                     /locus_tag="J1138_004846"
     CDS             complement(<1..602)
                     /locus_tag="J1138_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBA8456505.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDDNVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          189 a          170 c          156 g          195 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata
       61 gggatctcac gcaactccac atcccagtag cgggcgaatt tatgccagca gatttgtacc
      121 ggaccgcaca ccaggttcgg tttgttagtc ggcttacctg cagcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggtgca ggcgcatgcc acagatcggc aaccatgttt
      301 acgcaacgca gatcgattgc tgcagattgc ggatattctt ctttgtcgat ccagtttttg
      361 ttaatggata aatccatcaa cttatggaca ttatcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      481 acatcatcac gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      541 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      601 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      661 agcaagtgca aaggactcgg ttttaaataa caaaatccta atgttattta
//
LOCUS       DADTFG010000395          686 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.399, whole genome shotgun sequence.
ACCESSION   DADTFG010000395 DADTFG010000000
VERSION     DADTFG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.399"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>686
                     /locus_tag="J1138_004847"
     CDS             <1..>686
                     /locus_tag="J1138_004847"
                     /inference="COORDINATES: protein motif:HMM:NF020698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456506.1"
                     /translation="FKVVDKDGNPAPAGTITVSPATEITPPGTYTATLTGEKAGEYTV
                     IPQYNDSAVGSLDEKIELKADETPDAGQGNSTIAAAPKSIAADNTAMSTLTFTAKDKK
                     GNPITDLTAVTFVVKDASGNVPTSGITVSATTNAGNGTYTATLNGTLAGTYTVTPSNN
                     GAAVGSLSDTVTLTAGTTPNASLSTFSADPGAIIPDGQDSATLTLHLQDSYGNAITGL
                     TNVTFAATPA"
BASE COUNT          159 a          218 c          222 g           87 t
ORIGIN      
        1 ccttcaaggt ggtggacaag gatggcaatc cggcgccggc ggggaccatt accgtctcgc
       61 ctgcaacgga aatcaccccg ccgggcacgt acacggcgac gctgaccggt gaaaaggccg
      121 gggagtacac ggtgataccg cagtacaacg acagtgcagt gggcagcctg gacgagaaga
      181 ttgagctgaa ggcggacgag acgccggacg ccggtcaggg taactcgacg atagcggcgg
      241 caccgaagag cattgcggcg gataacaccg cgatgtcgac gctgacgttc acggcgaagg
      301 acaaaaaagg caaccctatc accgacctta ctgcggtaac gttcgtggtc aaggacgcga
      361 gcggcaacgt gccgaccagc ggtatcacgg tgagtgcgac caccaatgcg ggcaacggca
      421 cctacacggc aacgctgaac gggacgctgg cggggaccta caccgtcacg ccgagcaaca
      481 acggtgctgc ggtgggtagc ttgtctgaca cggtgacgct gacggcaggg accaccccga
      541 acgcctccct gtcgacgttc agtgcggacc cgggtgccat catcccggac ggccaggaca
      601 gcgccacgct aaccctgcac ctccaggaca gttacggcaa cgccatcacc ggcctgacca
      661 acgtgacgtt cgcagccacg ccggca
//
LOCUS       DADTFG010000396          683 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.400, whole genome shotgun sequence.
ACCESSION   DADTFG010000396 DADTFG010000000
VERSION     DADTFG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.400"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            193..>683
                     /locus_tag="J1138_004848"
     CDS             193..>683
                     /locus_tag="J1138_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012005178.1"
                     /note="is involved in outer membrane permeability; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HBA8456507.1"
                     /translation="MKKSFPTLLATMIWTALYSQHALADLAEQCMLGVPVYDKPLVSG
                     DPNSQPVTINADDSRADYPKSALFSGNVHIEQGNSTLTAKEVELNQTQNPGQTEPVRT
                     VTATGDVHYSDNQIKLKGPKAWSNLNTKDTDVYEGDYQMVGRQGRGDADKMKMRGANR
                     YTIL"
BASE COUNT          182 a          196 c          175 g          130 t
ORIGIN      
        1 tcctccgttt caggaaaggg cgcaggcgat ttttcctaga atttggatta tcatacctgt
       61 catttatctt tgcgcctaac gacgacgcaa gtaaacggct ggatttagca ctagcgcaac
      121 gcagatgagt aatatagtct ctgaccgttt gccggacatg acgcctctga tgacggaaca
      181 ccaaaaccgc gtatgaaaaa aagtttccca acactgctgg ccacgatgat ttggacggca
      241 ctctacagtc agcatgcgct ggctgatctg gccgagcaat gcatgcttgg cgttcctgtt
      301 tacgacaagc ctttggtcag cggcgatccg aatagccagc cggtgaccat caatgccgat
      361 gactcgcgtg ccgactaccc gaaaagcgcc ctgttcagtg gcaacgtgca tatcgagcag
      421 ggcaacagca ccctgaccgc caaagaagtt gagctgaatc agacgcaaaa ccccggccag
      481 acggaaccgg ttcgtaccgt caccgccacc ggcgacgtgc actacagcga caatcagatc
      541 aaactgaaag gcccaaaggc ctggtctaac ctgaatacca aagataccga cgtctatgaa
      601 ggcgactacc agatggtagg ccgtcagggg cgcggcgacg ccgacaagat gaaaatgcgc
      661 ggcgccaacc gttacaccat cct
//
LOCUS       DADTFG010000397          663 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.402, whole genome shotgun sequence.
ACCESSION   DADTFG010000397 DADTFG010000000
VERSION     DADTFG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.402"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            18..590
                     /locus_tag="J1138_004849"
     CDS             18..590
                     /locus_tag="J1138_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456508.1"
                     /translation="MIMDTNNGDQIAREHFASFLWGFMIPAKIPEENISGKVFVEPVV
                     LEGGAGGNWTVFDEVLDSNVIKQLTLTGCGAACGEMLLRDRYIFVTQNVIGTELTSMT
                     SLANKLNKFDVGWEGNAVSESSLYALSNTGSWGAMMWDSGSKVGHWVLVKGVDDAGNV
                     IIYDPYQGSRYLMTEQEFKEVWNGHSVYKP"
BASE COUNT          208 a           76 c          179 g          200 t
ORIGIN      
        1 gggatgcact taaaaaaatg ataatggata caaataatgg tgatcagatt gctcgggagc
       61 attttgccag ctttttatgg ggctttatga tacccgcaaa aataccagaa gaaaatatct
      121 ctgggaaagt atttgtagag ccagtagttt tagaaggtgg tgctggtggc aattggacag
      181 tatttgatga ggttttagat tcaaatgtaa taaagcaatt gactttaaca ggctgtggag
      241 cggcctgtgg ggaaatgttg cttagggata ggtatatttt tgtcacgcaa aatgtaattg
      301 gaacagaatt aacatctatg acgtcgctgg caaacaagct taataaattt gatgtcggct
      361 gggaagggaa tgcagtgagc gaatctagtt tatatgccct cagtaatact ggttcatggg
      421 gagcaatgat gtgggattct ggaagtaaag tagggcattg ggtacttgtt aagggtgttg
      481 atgatgctgg gaatgtgata atatatgatc catatcaagg gagtcgatat ctgatgactg
      541 aacaggaatt taaggaggta tggaatgggc atagtgttta caaaccataa tattgatttg
      601 ttatcggttg aatttgatga gattacaaaa aattgtaatt acacttttag tgtagatgga
      661 gag
//
LOCUS       DADTFG010000398          661 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.403, whole genome shotgun sequence.
ACCESSION   DADTFG010000398 DADTFG010000000
VERSION     DADTFG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.403"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..595)
                     /locus_tag="J1138_004850"
     CDS             complement(<1..595)
                     /locus_tag="J1138_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456509.1"
                     /translation="MDVLLWKLGKNDSRRRSGVYYLNAYKDAYVQYNRDKIIKHAYAA
                     GIRPELLGGVAWIESGGMPENYKFQIYETKRMIGLLDMPENKTSFGSMGIQIRTAAIT
                     LGLDPSELTTRNQLELATCLMEDDFTFQISATYLRDLVLFDYPSSATLYMTNEQYIMA
                     GIRYKRGVERDLVFFICLINNLPARDTDDYKFISYGMR"
BASE COUNT          209 a          137 c          101 g          214 t
ORIGIN      
        1 acctcatacc ataagagatg aatttataat catctgtatc acgcgcaggc aaattattaa
       61 ttaaacaaat aaaaaaaacc aaatctcgtt caacaccacg cttatatcta ataccagcca
      121 taatatactg ctcattcgtc atatacagtg ttgccgaaga gggataatca aaaaggacaa
      181 gatctcttaa ataagtagca gaaatttgaa aggtaaaatc atcctccatt aggcatgtcg
      241 ctaactctaa ctgatttctg gtagttaatt ctgaagggtc tagacctaga gtaatcgctg
      301 cggttcgtat ttgtataccc attgagccaa atgatgtttt attttcaggc atatctaaca
      361 atccgatcat tctttttgtt tcatatatct gaaacttata attttcaggc atacctcctg
      421 actcaatcca ggccacgccg cccagtaact ctggacgaat tccagcggcg tatgcatgtt
      481 taattatttt gtctctatta tattgtacat atgcatcttt ataagcattt aaataatata
      541 ctcctgaacg gcgacgcgaa tcattttttc ctaacttcca gagtaaaacg tccatttcat
      601 cccatttacg gaacttcgct tgtggaattg tacaatatct attttttata gtgagatcgc
      661 t
//
LOCUS       DADTFG010000399          659 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.404, whole genome shotgun sequence.
ACCESSION   DADTFG010000399 DADTFG010000000
VERSION     DADTFG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.404"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>659
                     /gene="galE"
                     /locus_tag="J1138_004851"
     CDS             <1..>659
                     /gene="galE"
                     /locus_tag="J1138_004851"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBA8456510.1"
                     /translation="IFSSSATVYGAPSRIPLDESCDTGDTTNPYGTSKYFLEQILIDT
                     QRANPALNIMLLRYFNPVGAHSSGFIGEDPNGIPNNLFPYITQVAIGRLPRLSIFGDD
                     YPTEDGTGVRDYIHVMDLASGHVAALNRQHVLTGLRCYNLGTGKGYSVKEIVETFQKI
                     TGKKIPYIVSARRPGDIAECWSSPELAQKELHWEAKRSLEDMVIDAWNWQNKNPNGYL
                     E"
BASE COUNT          200 a          110 c          143 g          206 t
ORIGIN      
        1 ttatctttag ttcatctgca acagtttatg gtgctccttc tagaattccg cttgatgagt
       61 cgtgtgatac tggagatact acaaatccat atggaacttc aaaatatttt ttggagcaga
      121 tattgattga tacacaacgt gctaatcctg cattaaatat tatgctgctg aggtatttta
      181 atcctgtcgg agcccattca tcaggtttca tcggcgagga cccgaatgga attccaaata
      241 atctatttcc atatattacc caagtggcca ttggaagatt accgaggttg tcaatatttg
      301 gagatgatta tccaaccgag gatggtactg gagtaagaga ttatatacat gttatggatt
      361 tagcatctgg tcatgttgct gctctcaata gacagcatgt tttaacggga ttgcggtgtt
      421 acaatcttgg cacagggaaa ggttattcag tcaaagagat tgttgaaact tttcaaaaga
      481 taactggaaa aaaaatacca tatattgttt ctgcacgtcg tcccggtgat atagcagaat
      541 gttggtcctc accagagtta gctcaaaaag aacttcattg ggaagctaag cgtagtcttg
      601 aggatatggt tattgatgct tggaactggc aaaataagaa tccaaatgga tatttagaa
//
LOCUS       DADTFG010000400          643 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.405, whole genome shotgun sequence.
ACCESSION   DADTFG010000400 DADTFG010000000
VERSION     DADTFG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.405"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            59..>643
                     /locus_tag="J1138_004852"
     CDS             59..>643
                     /locus_tag="J1138_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HBA8456511.1"
                     /translation="MVLITTTKNTFIIQAIKSMRWRGFSYVKVIAVDDYSSLIRDFGT
                     SCFRDEQSLLLPVFPDNDSTTCIRSLYFIHQWLKSQPVNIPCLAWGDNGVVSNKGIPF
                     IPWKLTAEQFCFYIDKFINGWRCKKNIRANHKVSFLERQLSPREKSIFIYTKEGKDIA
                     WISNTLGISSKTVWTHRRRAMDKLGVKRLHQFINI"
BASE COUNT          197 a          110 c          141 g          195 t
ORIGIN      
        1 ccccccggtg tgcaaagcaa aaggaaattg ggaaagggga taacaggaaa ttgattgcat
       61 ggttttgatt acgactacaa aaaatacttt tataattcaa gcaattaaga gtatgcgttg
      121 gcggggtttt tcgtacgtga aagtcatcgc tgtggacgac tattcttctc tgattcgtga
      181 ttttggcaca tcttgctttc gggatgagca atcgttgttg ttacctgtat tccctgataa
      241 tgattctact acctgtatac gctcactgta tttcattcac caatggctaa aaagccagcc
      301 tgtcaatatt ccgtgcctgg catgggggga taatggtgta gttagtaata aaggcatacc
      361 tttcataccg tggaaattaa cagcagagca attttgtttc tatatagata aatttatcaa
      421 tggctggagg tgcaaaaaaa atattcgcgc gaatcataaa gtttcttttt tagaacgaca
      481 attatccccg agggagaaaa gtatctttat atataccaag gaaggaaaag atattgcatg
      541 gatctcgaac accttgggta tatccagtaa gactgtgtgg acgcatcgtc gtagagctat
      601 ggataaactc ggggtaaaac gtttacatca atttataaat att
//
LOCUS       DADTFG010000401          642 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.406, whole genome shotgun sequence.
ACCESSION   DADTFG010000401 DADTFG010000000
VERSION     DADTFG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.406"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>642
                     /locus_tag="J1138_004853"
     CDS             <1..>642
                     /locus_tag="J1138_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBA8456512.1"
                     /translation="HELASQIYNQLKFSGTVSNCFDVLKNAVDDKLLDLNPVIAEQLM
                     LAFKAISSDKEEEWSQALTTCRRLLEGLADELYPASKEKFNGRAVGQGQYVNRLWAFM
                     DGAIQSDSNKDLAKAHIDFLGSWLDKVNKLTNKGVHAELDRIEAVKSVFHTYLVVADL
                     LEYMSNTKTSVSKPDINKATLDELEALLNINRTIAKEIVKARVREGKLDLDIL"
BASE COUNT          218 a           96 c          134 g          194 t
ORIGIN      
        1 cacatgaatt agcatcgcaa atatataatc aactaaaatt ttcaggtact gtcagcaact
       61 gttttgatgt attgaaaaat gctgttgatg ataaattatt agatctaaat ccagtgatcg
      121 cagaacaact aatgttggca tttaaagcta tttcttcaga taaagaagaa gaatggtctc
      181 aagctttaac tacatgtcgt aggcttctag aagggttggc tgatgaactt tatccggcat
      241 cgaaggaaaa atttaatggt cgggccgttg ggcaaggtca atacgtaaac cgcctttggg
      301 catttatgga tggggctatt cagtctgata gtaataaaga ccttgccaaa gcacatatag
      361 attttctcgg atcatggtta gataaagtta ataagttaac aaataaaggt gttcatgccg
      421 agttagatcg gattgaagct gtaaagtcag tatttcacac gtatttagta gtggcggatt
      481 tgcttgagta tatgtcaaat accaaaacct ctgtatctaa gcctgatatc aataaagcta
      541 ctttagatga actggaagca ttgttaaata taaatagaac aatcgctaaa gaaattgtta
      601 aagcacgagt tcgtgaaggc aagcttgatc tggatatttt aa
//
LOCUS       DADTFG010000402          634 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.407, whole genome shotgun sequence.
ACCESSION   DADTFG010000402 DADTFG010000000
VERSION     DADTFG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.407"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..68
                     /locus_tag="J1138_004854"
     CDS             <1..68
                     /locus_tag="J1138_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBA8456513.1"
                     /translation="FKKKIKKAIRDVKVKLGLKSK"
     gene            93..>634
                     /locus_tag="J1138_004855"
     CDS             93..>634
                     /locus_tag="J1138_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBA8456514.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNS"
BASE COUNT          222 a           85 c          121 g          206 t
ORIGIN      
        1 tattcaaaaa gaaaattaaa aaagcaatca gggatgtaaa agtcaaacta ggattgaaga
       61 gcaaataata attatgatga agggtaatta aattggattt taaacatctt actcaattta
      121 aagatataat tgaactggac aagcgccccg ttaaacttga tgaacgggaa acgtttaatg
      181 tctcatgggg tattgatgag aattaccaag ttggggctgc gatttcaatt gcttcaattc
      241 ttgaaaataa taagcaaaac aaatttacct ttcacataat cgctgattac ctagacaaag
      301 agtatattga attattatca caattagcaa cgaagtatca aacagtaatt aaattatatc
      361 atattgattc tgagccattg aaggagctac ctcaatcaaa tatctggcca gtatctattt
      421 attatcgttt gctttcattt gattattttt ctgcgcgatt ggattcatta ttatatcttg
      481 atgctgatat cgtctgtaag gggtcattga acgagttaat agcattagag tttaaagatg
      541 aatatggggc cgtggtaatt gatgtagatg ccatgcaaag taaaagtgct gagcgtttgt
      601 gtaatgagga ttttaacggt agctatttta actc
//
LOCUS       DADTFG010000403          633 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.408, whole genome shotgun sequence.
ACCESSION   DADTFG010000403 DADTFG010000000
VERSION     DADTFG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.408"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            209..379
                     /locus_tag="J1138_004856"
                     /pseudo
     CDS             209..379
                     /locus_tag="J1138_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          199 a          150 c          128 g          156 t
ORIGIN      
        1 tattctaaag aacccgccca caaggcgggt ttttgctttt ggatctttat ataaaataaa
       61 cttgggagca taacgccatc aggtcttaaa cactcccgaa tcatcaataa acatcccccg
      121 ttaacaaaac acagttcagt tcacataaca tccctgtaat cccacacata tctgctggcc
      181 gaacgacgcc agtgcgcgta cggaggcaag agcagccctg cacatggcag cgcttgtggt
      241 aacctggttt aatccggtga taaaggcatt ttacatgcat ctgtcagcag ccggaaaaac
      301 caaaaaagtg gcgctggttg actgtatgcg taaattgctg accatcctga acgcaatgct
      361 cagaaagaac gaagagtgaa atgaatcgta gcatcatgtt gctccataat ttttacgttc
      421 aagacagttg attatcggcg catatgactc aaaaagagcc tgatgattgc ctcttttcca
      481 taggctcttt atctgaagtg aaggaaggcc cggaagaaag cactcgctgg catacgtgac
      541 tccagctcaa ccggagataa tcaccactgt tatttttcta tttaataata atctcctgaa
      601 aaacagatat agcaaaaata caatccggcc aga
//
LOCUS       DADTFG010000404          618 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.410, whole genome shotgun sequence.
ACCESSION   DADTFG010000404 DADTFG010000000
VERSION     DADTFG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.410"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          204 a          123 c           97 g          194 t
ORIGIN      
        1 catgattttc cctcttattt ttaaactact ttttgaaaaa atctttatct ctgtgctgac
       61 gcacggctac gccctgacgc tgacattttt attttattca tctcctgtct gttaaataaa
      121 aagaaaagca ctcgatcatt gtttgatctc ttttcctcat gatgctgaat ttagatccta
      181 tgtttaaaca tagtttttcc ctttagacat atagcatttg ctcttaaacg tataggaaaa
      241 ctacatcctt ggtagcagcg cttcgtgatt gttgcgaaca atcactctgc tgaacagggc
      301 ttgcccctgt taatattgga tttatccaaa aaagatcgcg tcgtagacgc atataaagtt
      361 aaagtaaaat taatcaagca gtcccgatag tagcagcggt tcatgattgt agaaaacaat
      421 cacttcgctg taataacata gtaggaaaaa taacacagat aacaccactg gtgccatttt
      481 gataaggcac caatcaacta aagagaagaa ctattttcaa tattacattc tttaaaatgt
      541 catccaaagc ttcgctattt gaataatgaa gaagaaaaat gctctcaagc cagctatagc
      601 ttaaaacaaa gtacgcac
//
LOCUS       DADTFG010000405          613 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.411, whole genome shotgun sequence.
ACCESSION   DADTFG010000405 DADTFG010000000
VERSION     DADTFG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.411"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..279
                     /locus_tag="J1138_004857"
     CDS             <1..279
                     /locus_tag="J1138_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456515.1"
                     /translation="PQYDTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFV
                     PDALPVVRKASWHCRQCHHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            267..>613
                     /locus_tag="J1138_004858"
     CDS             267..>613
                     /locus_tag="J1138_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456516.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPR"
BASE COUNT          164 a          142 c          175 g          132 t
ORIGIN      
        1 ccgcaatacg acactgaact tccctggttt gaacacactg acgacaggct gacagagcac
       61 ggtcaacagt gcccttatgt caggccggag cgcagagaaa tacagttgat taaacgtctg
      121 cagcaattcg taccggatgc cttacccgtg gtgcgtaaag ccagctggca ctgcagacaa
      181 tgtcaccacg attattatgg ggagcagtac tgcacaaact gccagaccgg aggattcagt
      241 attccccgga caactcagga ggaaatatgc gaattctgaa ctgttatatg gcgaatgaca
      301 gcaaaggcca ttttgttacg gcgaaagaag ctgcgaagca caaccgacag gacgttttgt
      361 gctgtgtgtc ctgtggatgc ccgttaacac ttcagcgggg caatgacgga caaccaccgt
      421 ggtttgaaca tgaccagatg actgtcgctg aaaaaatcct gctgcgatgt acctggcttg
      481 acccggcaga gaaagaggcc cgtcgtttgc atctgcaggg catgacggtt ccggattata
      541 cggtgaaggt gagaaagtgg ttttgtgtga tgtgtgacga agattatgag ggggaaaagt
      601 gctgcccacg ctg
//
LOCUS       DADTFG010000406          600 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.413, whole genome shotgun sequence.
ACCESSION   DADTFG010000406 DADTFG010000000
VERSION     DADTFG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.413"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..589
                     /locus_tag="J1138_004859"
     CDS             <1..589
                     /locus_tag="J1138_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
                     /protein_id="HBA8456517.1"
                     /translation="LKSPVRLKKYRSYRGNMGLAAENILQRQFKAEAPCEKWVTDITE
                     FRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVKRMLNKGLESLAEGEKPLLHSDQ
                     GWHYRIKSYQSALADRGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRNVEELE
                     NAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG"
BASE COUNT          183 a          125 c          168 g          124 t
ORIGIN      
        1 cctgaagtct cctgtgcgcc tgaagaaata ccggtcttac cggggaaata tgggactcgc
       61 tgcagaaaat atccttcagc gacagttcaa agcggaagct ccctgtgaga aatgggtgac
      121 agatatcacc gaattcaggg ctggcggaca aaaactgtac ctgtccccga tacttgattt
      181 gtttaacggg gaaatcgtgg cgtgggaaac ggcctgcagg cctacagagg agctggtgaa
      241 gcggatgtta aataagggtc tggaaagcct tgctgaaggg gagaaaccgc tgctccacag
      301 tgatcaagga tggcattacc ggataaaaag ttatcagtcc gccctggcgg acagagggtt
      361 agtgcagagc atgtcgcgca agggcaactg ccttgataat gcggtaatgg agaacttctt
      421 tggtcacctg aaagaagaaa tgtactatcg ccgggactac agaaatgtag aagagctgga
      481 aaatgccgtt aacgaataca taacttactg gaatcaaaaa agaataaaac tcagtcttgg
      541 gggactcagc ccggtagaat accggactga gtatcaaaaa gccggttaac tgaaactgtc
//
LOCUS       DADTFG010000407          584 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.415, whole genome shotgun sequence.
ACCESSION   DADTFG010000407 DADTFG010000000
VERSION     DADTFG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.415"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..547
                     /locus_tag="J1138_004860"
     CDS             <1..547
                     /locus_tag="J1138_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA8456518.1"
                     /translation="EWLLFFEAINGSKNEIRDKAMLQMAYVHGLRVSELIALKISDID
                     FSESAIYIKRLKNGLSTVHPLQKETVLLLKKWLALRDNIVKKPFEDSLFLSCQGNKIS
                     RQYVYKMCKKYSHNMNINIHPHMLRHGCGYALANQGLDTRLIQDYLGHRNIHHTVLYT
                     ASNAARFKRVWEGDVLDIKKI"
BASE COUNT          210 a           87 c          111 g          176 t
ORIGIN      
        1 tgaatggttg ttattttttg aagccatcaa cggttcaaaa aatgaaatta gagataaagc
       61 catgttacaa atggcatatg tacatggtct tagggtaagc gagcttattg cactaaagat
      121 tagcgacatt gacttttcag agtcagcaat atatatcaag cgtttaaaaa atggattatc
      181 cacagtacat cccctgcaaa aagaaactgt tctgttatta aaaaaatggc tagcactacg
      241 tgataacata gtaaaaaaac cttttgaaga ctctttgttt ctttcttgtc aaggaaataa
      301 aatctcgaga caatatgtgt ataagatgtg taagaaatat agtcacaata tgaatattaa
      361 tattcatcct cacatgctta ggcatggttg tgggtatgct ttagctaatc aagggttaga
      421 caccagacta atacaagatt atttagggca cagaaatata catcatacgg tattatatac
      481 agccagtaat gcagcaagat ttaaacgagt atgggagggt gatgtgttag atataaagaa
      541 gatataaccc ctgttctaaa ggcctcaatt cattgaggct ttta
//
LOCUS       DADTFG010000408          582 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.416, whole genome shotgun sequence.
ACCESSION   DADTFG010000408 DADTFG010000000
VERSION     DADTFG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.416"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            131..>582
                     /locus_tag="J1138_004861"
     CDS             131..>582
                     /locus_tag="J1138_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456519.1"
                     /translation="MSESSQSLFMIHNMPSWVTIPEAAEITMKTLKRKIIPGDIYRHA
                     LSGDILLSIYFQSPVIIKKIQTFNGKVKFRQFDGGLIDKLCQLDKNGFIYEHNLTLCT
                     EGKYIHPTPRIIDTTLMGYEYVLIQRILAHEFKFPLPVTGAKELNYGIT"
BASE COUNT          193 a          115 c          101 g          173 t
ORIGIN      
        1 gcaaatgaat tcgtagcgat agacatcatt actcggccag caacttacct gataataaag
       61 atatcaacac tgctaacata taaaatagtt cctttccgga acaggtagcg gtgttaaagg
      121 agctaacatt atgtcagagt catctcaatc attatttatg attcataata tgccttcctg
      181 ggtcactata cctgaagccg ccgaaataac aatgaaaact ttaaagagaa aaatcattcc
      241 tggtgacatt tatcgccacg cattgagcgg agacattctt ctttcaattt attttcagtc
      301 cccagttatc attaagaaga tacagacatt taatggaaaa gttaaattca gacagtttga
      361 cggtggattg attgataaac tgtgccagct tgacaagaat gggtttattt atgagcataa
      421 cttaactctt tgcaccgaag gtaaatatat ccacccaacc ccgcgaatta tagatacaac
      481 ccttatgggt tacgaatatg ttctcatcca gagaattctt gctcatgagt ttaaatttcc
      541 tttgcctgtt acaggtgcaa aagaactcaa ctatggaatt ac
//
LOCUS       DADTFG010000409          582 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.417, whole genome shotgun sequence.
ACCESSION   DADTFG010000409 DADTFG010000000
VERSION     DADTFG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.417"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            322..552
                     /locus_tag="J1138_004862"
                     /pseudo
     CDS             322..552
                     /locus_tag="J1138_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          145 a          130 c          147 g          160 t
ORIGIN      
        1 ggattgttgg acatagaatg gagcagaagc cgggaatgaa gatattgccc ggttatccca
       61 ctgatgcatg agcatatcaa tatgctcagg cattatacgt tcactctccc ggaggatatt
      121 ttaaagggat aatcgagagc attaaatttc aatacaaaca acgaattatc ttcttagcgt
      181 acgttttcgt tccattggcc ctcaaacccc ctgacctgat aagtattgag tgtcacgcca
      241 tttcctgttg gatcatgtac atggctctca gattacaaaa ccggccagaa caatcagcac
      301 caggcggact gacttattgt cgtctcatat cgtgagctgc aggagatgct ggctgaacgt
      361 ggagtgaatg tcgatcactc cacggtttat cgttgggttc agctttatgc gtctgagatg
      421 gataaacggc tgcgttggta ctggcgtaac ccttccgatc tttgcctgtg gcacatggat
      481 gaaacctacg tgaaggtcaa tggccgctgg gcgtatctgt accgagccgt cgacaacagg
      541 ggtcacactg tcattggggc tagattttgg cttttcatgt ga
//
LOCUS       DADTFG010000410          575 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.418, whole genome shotgun sequence.
ACCESSION   DADTFG010000410 DADTFG010000000
VERSION     DADTFG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.418"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>575
                     /locus_tag="J1138_004863"
     CDS             <1..>575
                     /locus_tag="J1138_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456520.1"
                     /translation="KMIFTGKVSGEKTVLTAGGRHTVKAQPGEQYGLIDEVTGLVPDG
                     VEADRSGDDLILRKKEEDTEVRIEGFWEECQPGETQCTAVFNIVGEDGQVTEAVLTQD
                     GPVLDDITAGQSGTLSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGSKHRHENDDSDT
                     TAPSSPALKAEDDGSVSVVLPGDANKGDTVE"
BASE COUNT          144 a          105 c          185 g          141 t
ORIGIN      
        1 tgaaaatgat ttttaccggt aaggtcagcg gagaaaaaac cgtgctgact gccggaggtc
       61 gtcatacagt taaagcacag cccggggaac agtatggact tatcgatgag gtgaccggcc
      121 tggtgcctga tggtgttgag gcggatcgtt ccggtgatga tttaattctg cgtaaaaagg
      181 aagaagatac tgaggtccgg atagagggat tctgggaaga atgccagccc ggggaaacac
      241 agtgtacggc ggtatttaat attgttgggg aagatggaca ggtcaccgaa gctgtactga
      301 ctcaggatgg tccggtgctt gatgatatta ctgccggaca atcgggaacc ttgtcagatg
      361 atgatcgtgg cggttttata tggttgggcg gtctggcatt tggtggggga ctggctgcta
      421 tggctttagc tgcaggtggc ggtggaagca aacatagaca tgaaaatgat gactctgata
      481 ctacagcccc atcgtcacca gcactgaagg cagaggatga cggttctgtt tctgttgtct
      541 tgccgggtga tgccaataaa ggtgataccg ttgaa
//
LOCUS       DADTFG010000411          563 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.420, whole genome shotgun sequence.
ACCESSION   DADTFG010000411 DADTFG010000000
VERSION     DADTFG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.420"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            150..>563
                     /locus_tag="J1138_004864"
     CDS             150..>563
                     /locus_tag="J1138_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4755 domain-containing protein"
                     /protein_id="HBA8456521.1"
                     /translation="MEAVGIVFLVVLFIIIMTAVDIQKKKHYNSFTEVLDGDILSYEC
                     QQTGIVIDTQKHTVRIFNKDKDSTYTFDQIREINYTLSEGGKFYGNGTLRGMNNAAIA
                     NWREQLSANKRSGLNILTDDIKNPMWKVNVPLKNLS"
BASE COUNT          186 a           98 c          117 g          162 t
ORIGIN      
        1 tcttttatat cgatcatgat ttcaagttct gatagccttg caacgccgag accgacgcca
       61 tgagcttggt gctgtaggac gtataaaacg tagcaaagct ctgtgagcag gttcagtctc
      121 tttaactcat ttacatggag gaagtaagat tggaagcagt aggtatcgta tttttagtgg
      181 tgctcttcat catcatcatg acagcagtag atatacaaaa gaaaaagcac tacaacagct
      241 ttacggaagt tctggacggc gacattcttt cgtatgaatg ccaacagaca ggtattgtta
      301 ttgatactca aaaacatact gtccgtattt tcaataaaga taaagacagc acatatactt
      361 ttgatcaaat tcgagaaata aattatacgc tttcagaggg gggtaagttt tatggcaatg
      421 gaacattacg aggtatgaat aatgcggcta ttgctaactg gagagaacaa ctttcagcga
      481 ataaacggtc tggcctaaat attttaaccg atgatattaa aaatcccatg tggaaagtta
      541 atgttcccct gaaaaattta agc
//
LOCUS       DADTFG010000412          559 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.421, whole genome shotgun sequence.
ACCESSION   DADTFG010000412 DADTFG010000000
VERSION     DADTFG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.421"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..405
                     /locus_tag="J1138_004865"
     CDS             <1..405
                     /locus_tag="J1138_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M35"
                     /protein_id="HBA8456522.1"
                     /translation="DIFGRNEIEANKWLLRGVEAMILVNQRVKSNCFKYQGDECIFAK
                     VQGGISSENPVIVGPKFFHAAVKGYDSQVSTLCHEFSHLSEVLYTDDLSPPGKNAETT
                     SAEEYREHAENLVKAKSRDVMINGYNIERYFE"
BASE COUNT          189 a           78 c          121 g          171 t
ORIGIN      
        1 gatatttttg ggagaaatga gattgaggct aataaatggc tgctgagagg cgttgaggca
       61 atgatattag tcaatcagag agtgaaaagc aattgtttta aatatcaagg tgatgaatgt
      121 atttttgcca aagtgcaggg agggattagt agtgagaatc ctgttattgt aggccctaaa
      181 ttttttcatg ccgcggttaa aggatacgat tcccaggtat ccactttatg tcatgagttt
      241 tcacatttaa gcgaagttct gtatactgac gatttaagcc ctccgggtaa aaatgcggaa
      301 acaacatctg ctgaagaata cagagagcat gcggaaaatc ttgtaaaagc taaaagtcga
      361 gatgtaatga taaatggata taacattgaa cgttacttcg aataagatta ttcattgcct
      421 ttatatcaca acctttacgc taatgttttt cagcacaaat ttgaaaggtg aaaattattt
      481 aatacccaaa aaaaatgctt atggatcgtt agtattcgca gaaaagaagc atgaacatcc
      541 gggtgtttat gatttattt
//
LOCUS       DADTFG010000413          557 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.422, whole genome shotgun sequence.
ACCESSION   DADTFG010000413 DADTFG010000000
VERSION     DADTFG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.422"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..>557)
                     /locus_tag="J1138_004866"
     CDS             complement(<1..>557)
                     /locus_tag="J1138_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HBA8456523.1"
                     /translation="LLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYFNAGF
                     LLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISGKYNTRYS
                     INYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKP
                     VNSNQYRYCAKHKFKQKHYMAGIFN"
BASE COUNT          173 a          111 c           87 g          186 t
ORIGIN      
        1 attaaaaatg cctgccatat aatgcttttg tttaaattta tgttttgcac aataccgata
       61 ctgattgcta tttacaggtt taagtaaatc ttctttactc cacggagaag ctgctttggc
      121 gatcaaaaaa ctacgtgaca ccggataatc agcccactca tgccagggtt ttgtaggtcc
      181 cacatagtgt ataaacacgg tgtcatcatt gactggatta tcaactttgt cttttaattc
      241 atagttaata ctatatcggg tattatattt ccccgaaata aacttcactt taccattcaa
      301 taatacattc agtacatctt gatcaaggtg ggtgatttta cttacccaat ctgggtcacg
      361 caacatttca atagcttttg acgaaatgtt atttagattc cactcatcaa tatttatcag
      421 taaaaaacca gcattgaaat atccagaagc taattgtggt gtagttaata ccgaggctcg
      481 attctgccac cattcaacat ctctttcagc cacaactgcg gcaatttcat tagtagaaaa
      541 ttgataatct aagagtt
//
LOCUS       DADTFG010000414          554 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.424, whole genome shotgun sequence.
ACCESSION   DADTFG010000414 DADTFG010000000
VERSION     DADTFG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.424"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..>554)
                     /locus_tag="J1138_004867"
     CDS             complement(<1..>554)
                     /locus_tag="J1138_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBA8456524.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICT
                     GRPDEGIPAVCFKLKDGEDPGYTL"
BASE COUNT          127 a          158 c          159 g          110 t
ORIGIN      
        1 tacagggtgt atcccggatc ttcaccatct ttcagtttga agcaaaccgc cgggatgcct
       61 tcgtccgggc gaccggtaca gatgaactca tacggcccca gtttggcgat ttcatccgcc
      121 agataagcag caacctggta ggaagcgttc tgtactttgg tatagccttc acgaccgagg
      181 cgcaggaatt cataatactg tgcaatcacc tgacccgccg ggcgggagaa gttgatggcg
      241 aaagtaccaa tctgaccgcc gaggtagtca acgttgaaca ccagttcctg cggcagcgct
      301 tcttcatcac gccagataac ccagccgcag cccagcggag ccagaccgaa tttatggcct
      361 gaagcactga tcgatttcac acgcggcagg cggaagtccc agacgatatc cggggcgacg
      421 aacggtgcca ggaagccacc gctggcggcg tcgatgtgca tgtcgatgtc gataccggtg
      481 tcggcctgga atttatccag cgcatcgtgc agcggctgcg ggaactcata gttaccggtg
      541 taggtcacgc cgaa
//
LOCUS       DADTFG010000415          544 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.425, whole genome shotgun sequence.
ACCESSION   DADTFG010000415 DADTFG010000000
VERSION     DADTFG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.425"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            11..535
                     /locus_tag="J1138_004868"
     CDS             11..535
                     /locus_tag="J1138_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456525.1"
                     /translation="MIPVKVSSKKIRELAQKAKEILAAIDSGASEEDAFLKDMIDDWN
                     SQVVCPCEFSDFRDYSSWTNANEFTRIAFNLEKFYEDFTWEELVQTISCVCDQKSNES
                     EKIFALLLLEKNFHGNPSDLIFWPDEWFQDPDMLDVELSVEEIACYLMVRSGRQLVDA
                     PEIDLKYQIPQSDE"
BASE COUNT          183 a           83 c          119 g          159 t
ORIGIN      
        1 ccctgaacgt atgatccccg taaaagtcag cagcaaaaaa ataagagaat tagcacagaa
       61 agctaaagag atacttgcag ctattgatag tggagcaagt gaagaagatg cttttttaaa
      121 ggacatgatc gatgactgga acagtcaggt agtatgtcca tgtgagtttt cagattttag
      181 agattattcc tcttggacaa atgcaaatga atttacacga atagctttta atttggaaaa
      241 gttctacgag gattttactt gggaggaact ggttcaaacg ataagctgtg tatgtgatca
      301 aaagtcaaac gaatcggaaa aaatttttgc tcttttgttg cttgaaaaga attttcatgg
      361 aaatccatcc gatcttatat tctggcccga tgaatggttt caggatccag atatgttaga
      421 tgttgaactg agtgttgaag agattgcatg ctatttgatg gttcgatccg gtaggcaact
      481 agttgatgca cctgaaatcg acctgaaata tcaaataccc cagtctgacg aataaaaaat
      541 taat
//
LOCUS       DADTFG010000416          538 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.426, whole genome shotgun sequence.
ACCESSION   DADTFG010000416 DADTFG010000000
VERSION     DADTFG010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.426"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            163..>538
                     /locus_tag="J1138_004869"
     CDS             163..>538
                     /locus_tag="J1138_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBA8456526.1"
                     /translation="MIENLRNIAIIAHVDHGKTTLVDKLLQQSGTFGERAEATERVMD
                     SNDLEKERGITILAKNTAINWNGYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAM
                     DGPMPQTRFVTKKAFANGLKPIV"
BASE COUNT          135 a          129 c          148 g          126 t
ORIGIN      
        1 tgaaatggtg caaaagtgca acgattgcgg ctttttgcac cgtgaaaggg atcacaaact
       61 gaccggtacg cggttgttta taggagatgg ttgtgatctt gtttagtccc tcgcttaata
      121 cgtgtacaat agcgcgctat ttctaatgcc cgaggcaaag ctgtgatcga aaatttgcgt
      181 aacatcgcca ttattgccca cgttgaccat ggtaaaacca ccctggttga caagctgctg
      241 caacaatccg gtactttcgg agagcgtgcg gaagccaccg aacgcgtaat ggactccaac
      301 gatttggaga aagagcgtgg gattaccatc ctcgcaaaaa acaccgccat taattggaat
      361 ggttaccgca tcaacatcgt ggatacccca ggacacgccg acttcggcgg tgaggttgag
      421 cgcgtgatgt cgatggttga ctcggtgctg ctggttgtgg atgcaatgga tggccctatg
      481 ccgcaaaccc gcttcgtgac caaaaaggcg ttcgccaacg gtctgaagcc gatcgtgg
//
LOCUS       DADTFG010000417          515 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.427, whole genome shotgun sequence.
ACCESSION   DADTFG010000417 DADTFG010000000
VERSION     DADTFG010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.427"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..470)
                     /locus_tag="J1138_004870"
     CDS             complement(<1..470)
                     /locus_tag="J1138_004870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA8456527.1"
                     /translation="MSASAVLIGTGTSAIPLITAGLSKLQGGTRYIGRIMAGVLTGIL
                     FSRFLSSMLSEIWGWKSVYALSALVMFMSGIILFKDYPAPDNKEVKTHASYFHILFLN
                     IRGLTQDSVIRHYCFNAFAIMFLFSAFWSNVSMYLTTAFHFSQTQVGLFSLTGVA"
BASE COUNT          173 a          108 c          108 g          126 t
ORIGIN      
        1 gcaacaccgg tcagggagaa taatcctact tgcgtctgac tgaaatggaa agccgttgtc
       61 agatacattg aaacattact ccagaaagct gagaacagaa acataatggc aaatgcgtta
      121 aaacagtaat gccttataac agagtcctgt gtgagtcccc tgatattcag aaatagaata
      181 tggaaataag atgcatgtgt tttcacttcc ttattgtcgg gggcgggata atctttgaaa
      241 agtatgatgc cggacatgaa catcacgaga gcagacagag cgtatactga cttccagccc
      301 cagatttcac ttaacatact cgacagaaaa cgagaaaaaa gtattccggt caaaactcca
      361 gccataattc tgcctatata ccgggttcct ccctgcagct tgctcaagcc agctgtaatc
      421 agaggtatag cggatgttcc tgttccgata agaactgcag acgcagacaa taagtaataa
      481 ctcttcgtta tgaaaaaaat cagtaatgac attgt
//
LOCUS       DADTFG010000418          514 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.428, whole genome shotgun sequence.
ACCESSION   DADTFG010000418 DADTFG010000000
VERSION     DADTFG010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.428"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            29..>514
                     /locus_tag="J1138_004871"
     CDS             29..>514
                     /locus_tag="J1138_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HBA8456528.1"
                     /translation="MPADNVKDNSEVTAIAKDPSGNESDPSTVTSKTDGVADAPVLSI
                     PEVADGYANADELKDGLQAEVTLPAGTAEGAVITLTVTRPDKTTENVTHTVTKDEVAA
                     GKVSMDIPKDAVIDGQNSVSVSLTQGSNPAKPGNVVDFTADTQIPGDTDGDGATDATP
                     VV"
BASE COUNT          138 a          119 c          158 g           99 t
ORIGIN      
        1 cccggacagc aatggcgata aagcgaccat tccggcagat aatgtgaagg ataacagtga
       61 agtcaccgcc attgccaaag atccgagcgg taatgaaagc gatccttcaa ccgtgacgtc
      121 aaaaactgat ggtgtggcag atgccccggt cctgagcatt cctgaagtgg ctgacggtta
      181 tgccaatgcc gatgagctga aggatggttt gcaggcagag gtcacgttgc cggcaggcac
      241 ggcagaaggt gcggttatta cactgactgt gacccgtccg gataaaacca cggagaacgt
      301 gacgcatacc gtgactaaag atgaagtggc tgccggtaaa gtatccatgg atattccgaa
      361 ggatgcggtg attgacgggc agaacagcgt cagtgtcagt ctgactcagg gcagtaaccc
      421 ggcgaagccc gggaatgtgg ttgattttac tgccgatacc cagatcccgg gggataccga
      481 tggtgatggt gcaacagatg caacaccggt agta
//
LOCUS       DADTFG010000419          511 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.430, whole genome shotgun sequence.
ACCESSION   DADTFG010000419 DADTFG010000000
VERSION     DADTFG010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.430"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>511
                     /locus_tag="J1138_004872"
     CDS             <1..>511
                     /locus_tag="J1138_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HBA8456529.1"
                     /translation="TAAFTRSTDILRRAQEGIRIMKENMENYRHYQTRGLINKDQLTN
                     QVALYYQQQNNLLGLSGQNEQNALQITALESQIHIQAAEFDNQIYQMELQRYELQKER
                     LNIDAGGAIIVRALADGRIDSLSVTVGQMVNVGDSLLQVIPHDIDHYALVLWVPNDAI
                     PYIATGDKVN"
BASE COUNT          127 a          155 c          141 g           88 t
ORIGIN      
        1 accgctgcgt tcacccgctc cactgacatt ctccggcgtg ctcaggaagg gatacgcatc
       61 atgaaggaaa acatggagaa ctaccggcac tatcaaacca ggggactgat caacaaggat
      121 cagctgacca accaggtggc actgtattac cagcagcaga acaacctgct gggcctgtcc
      181 ggccagaatg aacagaatgc gctgcagatc accgcgctgg aaagccagat ccatatccag
      241 gccgccgaat ttgacaacca gatttaccag atggagctgc agcgctacga gctgcagaag
      301 gagcggctca acatcgatgc cggtggcgcg atcatcgttc gcgcgctggc agacggccgt
      361 atcgactcgt tgagcgtcac ggttggccag atggtcaacg tcggcgacag tctgctgcag
      421 gtgatccccc acgatatcga tcactacgcc ctggtgctat gggtgcccaa cgacgccatc
      481 ccctacatcg ccaccggtga caaggtcaat g
//
LOCUS       DADTFG010000420          507 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.431, whole genome shotgun sequence.
ACCESSION   DADTFG010000420 DADTFG010000000
VERSION     DADTFG010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.431"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>507
                     /gene="rfaY"
                     /locus_tag="J1138_004873"
     CDS             <1..>507
                     /gene="rfaY"
                     /locus_tag="J1138_004873"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBA8456530.1"
                     /translation="TCRVKTLKVFRSIADTKVILIDTARGPLVLKVYAPKHKMIERFL
                     KSCVKKDYYENLIYQTDRVRGEGIQSINDYYLLAERKTLNFAHYYIMLIEYIEGVGLN
                     EYLEISEDLKNQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKA
                     KDRIDLER"
BASE COUNT          176 a           74 c           94 g          163 t
ORIGIN      
        1 taacttgcag ggttaaaaca ttaaaagttt ttcgttctat tgctgatact aaagttattc
       61 ttattgacac tgctcgtgga cctcttgtac ttaaagttta tgcgccaaag cataaaatga
      121 tcgaacgttt cttaaaatct tgcgtaaaaa aagattatta tgaaaattta atttatcaaa
      181 ccgatcgggt acgtggtgaa ggcatccaat caattaatga ctattatttg cttgctgaac
      241 gtaaaacatt aaatttcgcg cattattata ttatgctcat tgagtatatt gaaggtgtcg
      301 ggttaaatga gtatttggaa atttctgaag atctgaagaa tcaattatca gaatcgataa
      361 aagagttgca tcagcatgga atggtgtctg gcgatcctca taaaggaaat tttatagtat
      421 ccgaaaaagg tttaagacta attgaccttt caggtaaaaa aacaaccgca gttttaaaag
      481 caaaagatcg cattgatctt gaacggc
//
LOCUS       DADTFG010000421          497 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.432, whole genome shotgun sequence.
ACCESSION   DADTFG010000421 DADTFG010000000
VERSION     DADTFG010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.432"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          148 a           92 c          116 g          141 t
ORIGIN      
        1 ctgtatccct ggtcaggttg acggggcact agtggtttca gcggttaatc gtcgaacagg
       61 cgcgagacaa tattcgcatt caacaaaata acaaacgcaa cactgatcgt tttgttgaga
      121 ggataactct ttttcagggt tatgggtgaa aggcctgaaa atttgcgatt atctttaaga
      181 gagtcacaaa tacactccaa aaccaaaacc cgctctctca ttctttagac tgaacttagc
      241 gtagctcacc atgtcaaaat gtttgaaact gctctggtgt gggaagtgtt cgaaaactcg
      301 tagattgtta tttcaatcag tgcgcaaaag agtgtcttac tgaaaaatga atatagatat
      361 tcatctggcg attcgagacg aaaagatttc tttcatccgc agttatgcgc atggttaaga
      421 ggtgacgatt gaagaagtcc tgatcttact gaagttacgc ggatagtggg taatgccgcg
      481 agattgatgg aacttta
//
LOCUS       DADTFG010000422          496 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.433, whole genome shotgun sequence.
ACCESSION   DADTFG010000422 DADTFG010000000
VERSION     DADTFG010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.433"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            95..340
                     /locus_tag="J1138_004874"
     CDS             95..340
                     /locus_tag="J1138_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBA8456531.1"
                     /translation="MQHVPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     EISLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
BASE COUNT          111 a          148 c          122 g          115 t
ORIGIN      
        1 tccacacccg tgccattcac agcatcgaca ccgatattaa gctcaaccgc gcattgtggg
       61 taatggcaga aacgctgctg gagagccagc gctgatgcag cacgtccctg aaggcgtgat
      121 tcaggcacca gaatcacccg acgccccgga ggcgctgccg gggtgtcgtt ccatttctga
      181 tgatgacctg tgcgcctggt gtacccgtct gctttaccgt cccggagaaa tcagtctgtg
      241 cagactgagt acaggcaatg gcatctggcc atccgtctgt gaccggaacg gttacgcgta
      301 cggctgtccg gaatttcagc cgaatatcat tcatccctga tattaccggg tctcacggtc
      361 agtgatgaac gtgatgttgc cagctcccga cctttactcc ctctcattta ttcacattac
      421 aaggatttca tacatgaaaa cattgtctca gaacaccacc tcttcggcct gtgcaccgga
      481 gactggcctg caacaa
//
LOCUS       DADTFG010000423          495 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.434, whole genome shotgun sequence.
ACCESSION   DADTFG010000423 DADTFG010000000
VERSION     DADTFG010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.434"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            54..>495
                     /gene="envZ"
                     /locus_tag="J1138_004875"
                     /gene_synonym="ompB"
     CDS             54..>495
                     /gene="envZ"
                     /locus_tag="J1138_004875"
                     /gene_synonym="ompB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="membrane-localized osmosensor; histidine kinase; in
                     high osmolarity EnvZ autophosphorylates itself and
                     transfers phosphoryl group to OmpR; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBA8456532.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDRLQLEDGTLLEVPPAFRREIYRELGISLYTNSAAEESGLRWA
                     QHYQFLSQQMAQQLGGPTDVRVEVNKNSPVVWLKTWLQPDIWVRV"
BASE COUNT           84 a          146 c          161 g          104 t
ORIGIN      
        1 atccagaccg tgtggggtct cggctacgtc ttcgtcccgg atggcagtaa ggcatgaggc
       61 gattgcgctt ttcaccgcgt agctcgtttg cccgaaccct gttgttgatc gtcaccttgc
      121 tgtttgtcag cctggtgacg acctatctgg tggtgctgaa cttcgccatc ctgcccagcc
      181 tgcagcagtt caacaaggta ttggcctacg aagtgcgtat gctgatgacc gatcggctgc
      241 agctggagga cggcacgctg ctggaggtgc cgccggcgtt ccgccgtgag atctaccgcg
      301 aactgggcat ctctctctac accaactcgg cggcggagga gagcggcctg cgctgggcgc
      361 aacactacca gttcctcagc cagcagatgg cgcagcagct cggcgggccg accgatgtgc
      421 gggtggaggt gaacaagaac tccccggtgg tgtggctgaa aacctggctg cagcccgata
      481 tctgggtgcg cgtgc
//
LOCUS       DADTFG010000424          495 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.435, whole genome shotgun sequence.
ACCESSION   DADTFG010000424 DADTFG010000000
VERSION     DADTFG010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.435"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          154 a           94 c           74 g          173 t
ORIGIN      
        1 ttaatgttta tattttcttg atttgttctt atttgaaata tattttcact ccctccttca
       61 taatgaaatc gattcatgag ttcctgtaaa gggttccacc tcccacgata agtctcacat
      121 aactgccacg acttatagat atacttccta acaatgccag aagtgttttt acactgctta
      181 tagtgtcgtt catccacaat agcaaaaaaa agatcataaa tatgctgctc taagaaccaa
      241 ttactattta ccaatgtgaa ctctacagtt cctatatcat attgatgctt cgcagggaga
      301 aaagcataat agtcattcaa atttactgtg tatgagagtt tttctccggg tgatatttct
      361 atccagtcag attttttcat ccacaaatca aatcggcatg taatttttct tatataatct
      421 aaccgtatgt tatttgttgc gattaaaaaa gaaccactgc acaaaggaga gtaaggtgat
      481 tcatggatgc ttgct
//
LOCUS       DADTFG010000425          493 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.436, whole genome shotgun sequence.
ACCESSION   DADTFG010000425 DADTFG010000000
VERSION     DADTFG010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.436"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(148..>493)
                     /locus_tag="J1138_004876"
     CDS             complement(148..>493)
                     /locus_tag="J1138_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456533.1"
                     /translation="KDLVATSNSQIRATKESLNIPRAVGGLVILNESIMPYESEYVMA
                     ELNFLVENPHYEHVDFVLYISELRRSTHNMIDMSAMIKSSSPRYEFVNWYIKNVFSFD
                     FASFFNHPIQFL"
BASE COUNT          185 a           83 c           74 g          151 t
ORIGIN      
        1 ttgaaggaca aaaaaactaa aactaaagag aacaaaatta attgccaagg caaaacacag
       61 tagtttcaag aaagagattt tctcaagcaa ctcctatctg aatgataact ccataaagcc
      121 aatgcatttt aacaaactcc caccctacta taaaaattgt atagggtgat taaaaaatga
      181 agcgaaatcg aatgagaaaa cgttttttat ataccagttt acaaactcat atcttggaga
      241 gctgcttttt atcattgctg acatatcaat catattatgt gttgatctac gtagttctga
      301 tatatagaga acaaaatcaa catgttcata atgtggattt tcaacaagaa aattaagttc
      361 tgccattaca tattcagatt cataaggcat tatcgattca ttaagaataa caagtccacc
      421 gactgctcta ggaatattta aagattcctt tgttgctcta atctggctat tggaggttgc
      481 tacaagatct ttt
//
LOCUS       DADTFG010000426          490 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.437, whole genome shotgun sequence.
ACCESSION   DADTFG010000426 DADTFG010000000
VERSION     DADTFG010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.437"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..180)
                     /locus_tag="J1138_004877"
     rRNA            complement(<1..180)
                     /locus_tag="J1138_004877"
                     /product="23S ribosomal RNA"
     gene            complement(378..453)
                     /locus_tag="J1138_004878"
     tRNA            complement(378..453)
                     /locus_tag="J1138_004878"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(418..420),aa:Ala,seq:tgc)
BASE COUNT          124 a          135 c           97 g          134 t
ORIGIN      
        1 ccactatgta ttcatgacat gatagtgtgt cgaaacacac tgggtttccc cattcgggta
       61 tcgccggtta taacggttca tatcacctta ccgacgcttt tcgcagatta gcacgccctt
      121 catcgcctct gactgcctag gcatccaccg tgtacgctta gtcacttaac ctcacaaccc
      181 gaagatgttt ccatcgttcg cgctgcaaac atttgagaga ctctatgaca ggttactctt
      241 tatcccaata catctacgga gggataaatt tcagccgtca tgtttcaatt ttcagcttgt
      301 tccagattgt taaagagcaa aatacttcgc agcatactgt cgccaatata ctctgaagta
      361 ttcaataccg gactatatgg tggagctaag cgggatcgaa ccgctgacct cctgcgtgca
      421 aggcaggcgc tctcccagct gagctatagc cccatacagt cacgtgcagt accttttcca
      481 cttacaagaa
//
LOCUS       DADTFG010000427          488 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.438, whole genome shotgun sequence.
ACCESSION   DADTFG010000427 DADTFG010000000
VERSION     DADTFG010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.438"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          162 a           70 c          101 g          155 t
ORIGIN      
        1 ttgagaagca atgaagtttc gttattaatc gatggggatg aacttgactc tctttatagc
       61 aagtaagcaa aaatacataa tggtaagcca tcgattgata cctggcggta gtttaggcgt
      121 taattattgg gcggataaag gatgtagttt gtcacatgcc agagaagtta cacggcgaat
      181 aaacccagta gcagatggct tcgatttagt acgttggcca gcatttgagc attcttgata
      241 tgtattgaat tatgaaataa ccccccccac ttaattacat aaggattgtt taaaacaatt
      301 gggtttagaa aaacaataat cacttcggta ggtttgatat ttatttcatt ctcttttgtg
      361 gcaaagtgct ctcaactcaa aaatttgaat aattactcag tgatgctttg tggaaaagtg
      421 tcaaataata tcctggatga tattggtggt tataaagaaa gaaatatatt aatgctgcaa
      481 gctataaa
//
LOCUS       DADTFG010000428          481 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.440, whole genome shotgun sequence.
ACCESSION   DADTFG010000428 DADTFG010000000
VERSION     DADTFG010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.440"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>481
                     /locus_tag="J1138_004879"
     CDS             <1..>481
                     /locus_tag="J1138_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456534.1"
                     /translation="HGFNYSSHSYNPNNWDLQITKDERESVQEYIINVLSSNDTLEKN
                     GTRIGAHNFKTKYNILNKKILFVPLQRPNDSVIRHFSDEIRSVQDFLNNVVTLAKSIK
                     DKGWVVIVKQHPLEESTEIGEKENLIVLKPTDHFYDAINSSDAVMLINSGVGLYSLMA
                     "
BASE COUNT          170 a           75 c           86 g          150 t
ORIGIN      
        1 catggtttca attactctag tcattcatat aaccctaata attgggatct gcaaattaca
       61 aaagatgaac gggaaagcgt tcaggaatat atcatcaacg ttctttcatc aaacgatacg
      121 ttagagaaaa atggtaccag aataggtgct cataatttca aaacaaaata taacatactc
      181 aataaaaaaa ttctttttgt tcctttgcaa agaccaaatg attctgtcat acgtcatttt
      241 tcggatgaaa ttagaagtgt gcaggatttt ttaaataatg tagttactct tgctaagagc
      301 ataaaggata aaggttgggt tgttattgta aaacaacatc ctttggaaga gtctaccgaa
      361 ataggcgaaa aagagaacct aattgtgctg aagccaaccg atcatttcta tgatgcaatt
      421 aattcttcag atgcagtaat gcttataaat tcaggagttg gtctttattc tctgatggca
      481 g
//
LOCUS       DADTFG010000429          478 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.441, whole genome shotgun sequence.
ACCESSION   DADTFG010000429 DADTFG010000000
VERSION     DADTFG010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.441"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(99..296)
                     /locus_tag="J1138_004880"
     CDS             complement(99..296)
                     /locus_tag="J1138_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456535.1"
                     /translation="MVIRKTVCRDCDNAIPHNTECCPGYVSADPFDYYRNTDRLLCLL
                     TLLPVLILAVVSGVSVFVLLQ"
BASE COUNT          120 a          127 c          117 g          114 t
ORIGIN      
        1 cggggagctg gtgcgttact gtcactgtct ggcacagcaa acgcagaggt ggcggggcgc
       61 tctgctccgc tgactccggc taatctctcc ccttttacct actgcaacag tacaaataca
      121 ctgacgcctg aaacgacagc cagaatcagc acaggcagta acgtcagtag acaaagcaga
      181 cgatcggtat ttcggtaata atcgaaggga tctgctgaca cgtagcccgg gcaacattcc
      241 gtattatgag ggatggcgtt atcacaatcc ctgcacaccg ttttgcgtat caccatcacg
      301 tcctcctcac aatcttatcc gtaaccggct ttttgcatta aaaaaacaac gctgaaaatt
      361 cagccgtcag tccggagacg acagtcgggt tatctcagag tgcctgagat attgcctggt
      421 tggagatcac ttcagggact ctgcgcattt tttacggcgt ggttaatccg tatgaacg
//
LOCUS       DADTFG010000430          477 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.442, whole genome shotgun sequence.
ACCESSION   DADTFG010000430 DADTFG010000000
VERSION     DADTFG010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.442"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>477
                     /locus_tag="J1138_004881"
     CDS             <1..>477
                     /locus_tag="J1138_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HBA8456536.1"
                     /translation="APGSAASLPPLVNGVTPEGEPMPTQFPPAPGGAVPPPTAFYPGK
                     GVTPPPSVDYKPIPVPGRLYRKSLVPAETGPRKKKYPYIPTGSFAKSILIEGADANAS
                     VTGNTATVPMQVRLTGRIEMPNSKTYDATGCFISMEAYGDVSSERAIVRTRKISCIR"
BASE COUNT           91 a          169 c          142 g           75 t
ORIGIN      
        1 gcccccggtt ccgccgcctc cttaccgccg ctggttaacg gcgtgacgcc cgagggcgag
       61 ccgatgccga cacagttccc gccggcaccg ggcggcgcgg ttccgccgcc gacggcgttc
      121 tatccgggca aaggcgtcac accgccaccg agcgttgact acaagcctat cccggtgccc
      181 ggccgtctct atcgcaagtc actggtgccg gcggagaccg gccccaggaa aaagaagtac
      241 ccctatatcc caaccggcag tttcgccaaa tccatcctga ttgagggggc ggatgccaac
      301 gcctcggtga ccggtaacac ggccacggtg ccgatgcagg tgcgcttgac cggccgcatc
      361 gagatgccga acagcaagac ctacgatgcg accggctgtt ttatcagcat ggaggcctac
      421 ggcgacgtct ccagcgaacg tgccatcgtg cgtacccgca aaatcagctg cattcgc
//
LOCUS       DADTFG010000431          474 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.443, whole genome shotgun sequence.
ACCESSION   DADTFG010000431 DADTFG010000000
VERSION     DADTFG010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.443"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            42..434
                     /gene="ydeI"
                     /locus_tag="J1138_004882"
     CDS             42..434
                     /gene="ydeI"
                     /locus_tag="J1138_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBA8456537.1"
                     /translation="MKFQAIVLASFLVMPYALADDPAGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTQLQK"
BASE COUNT          137 a          102 c          117 g          118 t
ORIGIN      
        1 cagacttaat aagtcaaacc aaacgcaatt aaggagtcag tatgaaattt caggccattg
       61 tattagcaag ttttcttgtc atgccttatg cgttggcaga tgaccccgcc ggtttaaaac
      121 aggatgcagc gccaccgccg cctcatgcaa tagaagatgg ctatcgcggt accgatgatg
      181 caaaaaaaat gaccgttgat ttcgcaaaaa ccatgcacga tggcgcttcg gtttcactac
      241 gcggtaattt gatttctcac aaaggagagg accgttacgt ttttcgcgat aagagcggtg
      301 aaattaatgt tgttattcct gcggccgttt ttgatggacg agaagttcag ccggaccaga
      361 tgatcaacat tagcggaagt ctggataaga aaagcgcgcc ggccgtcgtt cgggtcactc
      421 agttgcagaa ataaaaataa attagcctga tggcctgaac tactgggcca ttag
//
LOCUS       DADTFG010000432          468 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.444, whole genome shotgun sequence.
ACCESSION   DADTFG010000432 DADTFG010000000
VERSION     DADTFG010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.444"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>468
                     /locus_tag="J1138_004883"
     CDS             <1..>468
                     /locus_tag="J1138_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA8456538.1"
                     /translation="VHPRLTALRVDLRLPDVPAATDAAVISRFINALKARIDAYQKRK
                     HREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVNRDTWCRAGDYRAPGSLAGMIKQ
                     AWCSALGVDVGCHATLVHFPAWPAVWLERDDDAGLRDMLKRADYLAKEYTKVNG"
BASE COUNT           95 a          127 c          147 g           99 t
ORIGIN      
        1 tgttcatccc cgtctgacgg cattgcgggt tgatctgcgt ctcccggatg taccggcagc
       61 aacggacgcg gctgtgatat cccgcttcat caatgccctg aaagcccgaa tcgacgctta
      121 ccagaaacgt aagcatcggg aaggtaaacg cgtgcattcc tcgaccctgc attacgcctg
      181 ggcccgggag ttcggggagc ttaaaggcaa aaaacactat cacctgctgc tgctggtcaa
      241 ccgggatacc tggtgtcgtg ccggcgatta ccgcgctccg gggtcactgg ccggaatgat
      301 taaacaggcg tggtgcagtg ccctgggagt ggatgtcggg tgccatgcca cgctggtgca
      361 ttttccggcc tggccggcgg tgtggctgga acgcgatgat gatgctggtt tacgggatat
      421 gctgaagcgt gctgactatc tcgcgaaaga gtacaccaaa gtcaacgg
//
LOCUS       DADTFG010000433          466 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.445, whole genome shotgun sequence.
ACCESSION   DADTFG010000433 DADTFG010000000
VERSION     DADTFG010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.445"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..329
                     /locus_tag="J1138_004884"
     CDS             <1..329
                     /locus_tag="J1138_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA8456539.1"
                     /translation="NVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLS
                     LDDGGTNDLENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKN
                     IMEPTK"
BASE COUNT          187 a           81 c           70 g          128 t
ORIGIN      
        1 aaaatgtaag aaaaaatttt cttatcaaac ttgcaaaaac ctccgaagca tctggaagat
       61 tcaacgccga agacctttta agaatgagaa agggcaatgt tcctctaaat tataatgtac
      121 accataaact atctctagat gatggtggta ctaatgattt ggaaaattta gtattaatcg
      181 aaaacgaacc atatcataag gtttttacta acatgcaatc acgaatagct aagggaatat
      241 tagtgggtga aagcaaaatc actccctggg ccattccatc tggctcaatt tatcctccca
      301 tgaaaaatat tatggaaccc acaaaatgat gactaaaacc caaataaata aattaaaaaa
      361 aatgatgaat gatttagact atccatttga agcaccgctc aaggaatcat ttattgaaag
      421 tataatccaa ataaaattta attctaactc aactaattgc ctggag
//
LOCUS       DADTFG010000434          455 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.447, whole genome shotgun sequence.
ACCESSION   DADTFG010000434 DADTFG010000000
VERSION     DADTFG010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.447"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(206..>455)
                     /locus_tag="J1138_004885"
     CDS             complement(206..>455)
                     /locus_tag="J1138_004885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456540.1"
                     /translation="VKEKFNLNFDEVVFIDDINRVAEVCKALGTGFIGVPASMAHNYQ
                     REEMVRTGVKYMVNRFTDITDKLIHEVDEHLASSDLWK"
BASE COUNT          153 a           93 c           74 g          135 t
ORIGIN      
        1 atgataaaaa ataatgataa cgcattacca gtaagagcga atgaatacag catggaggct
       61 ctctcataag aaaccatcaa ctcactactg atcacaggag ccaaaggttg aacataatac
      121 atatttgctg ctgtaaaacc acatattatc ggtataagat aaatcagatg gttattgttg
      181 aacttttcca tgctgtacct tactgttatt tccagaggtc tgacgaagcc agatgttcgt
      241 caacttcatg aatgagttta tctgtaatat cagtaaaacg atttaccata tactttacac
      301 ctgttctgac catttcttct cgctgatagt tatgagccat ggatgcagga accccgataa
      361 acccagtacc taacgctttg caaacttcag caactcgatt gatgtcatca ataaaaacaa
      421 cctcatcaaa attaaggttg aatttttctt tgact
//
LOCUS       DADTFG010000435          455 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.448, whole genome shotgun sequence.
ACCESSION   DADTFG010000435 DADTFG010000000
VERSION     DADTFG010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.448"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>455
                     /locus_tag="J1138_004886"
     CDS             <1..>455
                     /locus_tag="J1138_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456541.1"
                     /translation="LSAIHHQIFENQLDTETITVSYEFILCQHLITNTEFSYLALPEK
                     YNRLFLPNSKNKTNNGLKTLNSEAVGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYD
                     QILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDSID"
BASE COUNT          169 a           84 c           82 g          120 t
ORIGIN      
        1 ttatcggcta ttcatcacca gatctttgaa aaccaactgg atacagaaac aataactgtt
       61 agttatgaat ttatcctctg ccaacatctt atcactaaca ccgagttcag ctatttggca
      121 ctccctgaaa agtataaccg cttgttttta ccaaattcaa aaaacaaaac aaataatggt
      181 ttaaaaacgt taaacagcga agctgttggc aggctacttg ctgctggtgg cgtatacaat
      241 gggaacattg aaggattcag agatactgcg gaaaaactgg gtggagatgc aatcaaaggc
      301 tatgatcaaa tactaaatga aaaaacagcg ggcatagcga tagcaacagc atctattctt
      361 ttaacaaagc gttctaatgt tgatacatat acagaaataa atagttactt aggcaaactt
      421 agaggccaac aaaaacttct tgatagtata gacat
//
LOCUS       DADTFG010000436          454 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.449, whole genome shotgun sequence.
ACCESSION   DADTFG010000436 DADTFG010000000
VERSION     DADTFG010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.449"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(23..346)
                     /locus_tag="J1138_004887"
     CDS             complement(23..346)
                     /locus_tag="J1138_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456542.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          132 a          110 c          119 g           93 t
ORIGIN      
        1 tccccctcgc tgattaaaac gtttagagaa agacagggaa ggcggaatga tggcactgat
       61 atgacaaagt gaattccgta ttcaaacata aggaacccgc aaagaggcta cagttgccta
      121 ctggctgacc aaaggggata gcgtgggtat agcctgcctg ctggcatgcg cgatcggcga
      181 tcccgcgact gacataacga tcggtacggg catgctgcaa aaaagcctga tcataaacca
      241 accgaacaat acctgttgcc gcgtcaactt cgctcacatg ggcctggcga gtaaccgtac
      301 atcctgtcag cgttattaag gaaagagtca gaatcagaag tctcatggtc cagccagaag
      361 agtaaaagaa agcagtattt tacgcgttac agcgctgcga caatggccgg aaaagacgcc
      421 tggcaggcgc tatttcttgc catctgtatg tgtc
//
LOCUS       DADTFG010000437          453 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.450, whole genome shotgun sequence.
ACCESSION   DADTFG010000437 DADTFG010000000
VERSION     DADTFG010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.450"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          156 a           82 c           67 g          148 t
ORIGIN      
        1 ttgaaagaag aagtaaaaag tttaatcact cctttttctc atacgcagtc acaggagcca
       61 taatggtttt ttgctctcaa ccattaataa aatacgcaaa cccaatgctt attttattga
      121 gtggtctgtt actgtctatt atcattatcc ttctcagaat gatttatatt tcacaagcga
      181 atgcaccatg gacaaccaaa aaacgttcac atgtactagt tcattttctt tctgcatgtt
      241 tcatagcgtc aacattgacg ttgttatatc aggcttacga taataacatc acacacaaat
      301 tgtactgtaa aaatatacaa caacttattg aaaaaaggat agaaacagaa aaaaatatca
      361 gcatattcag tgggatgcaa tgcaccccgg catatgatta ctctttattg ggatttaatc
      421 tcttataaag aatgttatta ctgatttgag tgc
//
LOCUS       DADTFG010000438          452 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.451, whole genome shotgun sequence.
ACCESSION   DADTFG010000438 DADTFG010000000
VERSION     DADTFG010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.451"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(40..115)
                     /locus_tag="J1138_004888"
     tRNA            complement(40..115)
                     /locus_tag="J1138_004888"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Thr,seq:ggt)
     gene            complement(130..245)
                     /gene="rrf"
                     /locus_tag="J1138_004889"
     rRNA            complement(130..245)
                     /gene="rrf"
                     /locus_tag="J1138_004889"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(330..>452)
                     /locus_tag="J1138_004890"
     rRNA            complement(330..>452)
                     /locus_tag="J1138_004890"
                     /product="23S ribosomal RNA"
BASE COUNT          103 a          136 c           97 g          116 t
ORIGIN      
        1 caaattcttt tctaatctgc cgaactttaa cctaaaaagt ggtgctgata cccagagtcg
       61 aactggggac ctcaccctta ccaagggtgc gctctaccaa ctgagccata tcagcacgct
      121 taatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
      181 ctacagcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctaaggccg
      241 ccaggcaaat tctgtttcat taacccgctt gtcgccggtt aatctaatct gtatcaggct
      301 gaaaatcttt ctctcatccg ccaaaacatc ttcggcgttg taaggttaag cctcacggtt
      361 cattagtacc ggttagctca acgcatcgct gcgcttacac acccggccta tcaacgtcgt
      421 cgtcttcaac gttccttcag gaccctcaag ga
//
LOCUS       DADTFG010000439          451 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.452, whole genome shotgun sequence.
ACCESSION   DADTFG010000439 DADTFG010000000
VERSION     DADTFG010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.452"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            116..409
                     /locus_tag="J1138_004891"
     CDS             116..409
                     /locus_tag="J1138_004891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456543.1"
                     /translation="MGFEELIDTLIADGHKTISLLSKMCLTDDGETFKVYLVLDQNGD
                     LVAKKICVPGGTGWSMTNWPVAQSGTHPDVEMQNVDLSDKATAKGYFSLFDCY"
BASE COUNT          135 a           87 c          111 g          118 t
ORIGIN      
        1 gaaatgataa gaatagaaat attcaggcga gtgactgact cgcggttgtg tgcaggcaga
       61 tctgctgcac gccataatta ccatttgtga ctattacact ggaaaggaat gactgatggg
      121 atttgaagaa ctgattgata cccttattgc tgacggccac aaaacaatat cgcttctttc
      181 aaaaatgtgt cttaccgacg atggggaaac ctttaaggtg tatctcgttc ttgatcaaaa
      241 cggagatctt gttgccaaga aaatatgtgt accgggaggt actggctggt caatgactaa
      301 ctggccagtg gctcagtcag ggactcatcc tgatgtggag atgcaaaacg ttgacctatc
      361 tgacaaagcg acagcaaaag ggtatttctc actgtttgat tgctactgac caatttcagg
      421 caggaaaaaa atgccggacg ggatttctca a
//
LOCUS       DADTFG010000440          434 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.453, whole genome shotgun sequence.
ACCESSION   DADTFG010000440 DADTFG010000000
VERSION     DADTFG010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.453"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(137..328)
                     /locus_tag="J1138_004892"
     CDS             complement(137..328)
                     /locus_tag="J1138_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBA8456544.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          133 a           84 c          113 g          104 t
ORIGIN      
        1 aacaggatga acttcagtca gaacaaagac gtaaaattta agatgaaaaa atatctgccg
       61 ctttgataat cccaaagagc aaaagcatcc gacagcgagg tgccgttatg ttctgtgaga
      121 gtggagaagg taaccgctaa cgttgtaccg gtgattcgac ggagagtgtg gattgctgag
      181 cttctttttt gctctgatgg tgctgccagg caccgacgga cgagtagata aaacggccaa
      241 agaagaagat aaagctgatg agcagtacga tacgggtcat gcgactgtta aatcggtgtc
      301 gttttcgcat actggttgcc tgactcacaa aaggttcctt gaagtatgtc ccacgccgtg
      361 gacggtactt acattaaggc acaacaggac aaaatggtca attcttcgtt atgtaaaaaa
      421 gcgtcagtgg atac
//
LOCUS       DADTFG010000441          433 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.454, whole genome shotgun sequence.
ACCESSION   DADTFG010000441 DADTFG010000000
VERSION     DADTFG010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.454"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            33..>433
                     /locus_tag="J1138_004893"
     CDS             33..>433
                     /locus_tag="J1138_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456545.1"
                     /translation="MKIYWTRKSIPELSTLPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILFRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILER
                     GKETETISERLIREADTREYELWRPVRRFFS"
BASE COUNT          131 a           81 c           81 g          140 t
ORIGIN      
        1 taaagtagaa cactttttat atgagttgct atatgaaaat atactggaca agaaaaagca
       61 ttcctgaact ttcaacactt ccaccttcat taagaaagaa aaattttact gacgcatata
      121 atgctgcatc ttcccatatc gaatactgga taggtgctgg cgtctcattt atctcgatga
      181 tgattttatt cagagtatat gactttcttt tacccaccca agatactttc ccgggtgata
      241 tcatcagaag tttatgtgtt gtgtgtccat ccatattaat ctggtttcag ttttcggtat
      301 atgtcatgag gaaatattac agacatattc tggaacgagg taaggaaacc gaaaccattt
      361 cagaaaggtt gatacgtgag gctgatacac gagaatatga actatggcgt ccggtccgtc
      421 gctttttctc cat
//
LOCUS       DADTFG010000442          421 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.455, whole genome shotgun sequence.
ACCESSION   DADTFG010000442 DADTFG010000000
VERSION     DADTFG010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.455"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..357
                     /locus_tag="J1138_004894"
     CDS             <1..357
                     /locus_tag="J1138_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4756 family protein"
                     /protein_id="HBA8456546.1"
                     /translation="IPLVKAQKFKSAHTEVRRLEKKRESLIEYFIDELNPISSSKANT
                     SARSTGNLDLFNERVLYRKAISEKSDEEIVALVIKQRTEAAVEFQRSIEQSLEQLSRI
                     SSEFEPSNQKRRKMSL"
BASE COUNT          145 a           80 c           80 g          116 t
ORIGIN      
        1 attccattgg ttaaggcgca aaaatttaaa tctgcccata ccgaagttcg caggttggaa
       61 aaaaagagag aatctcttat tgaatacttt attgacgagt taaatcccat cagcagttca
      121 aaagctaaca catcagcaag atcaactggt aatctcgatt tatttaacga acgcgtgtta
      181 taccgtaagg ccatttcaga aaagagcgat gaagaaatag tagccttggt cataaaacaa
      241 cgtacagaag ccgctgttga gttccaacgt tcaattgaac agagtcttga gcaactatct
      301 cgtatctcgt cagagttcga accatcaaac caaaagcgca ggaaaatgtc tctttaaaac
      361 gactctatgc tattcgtgtg aaatgtgatg tattagattg cttatgcaat aatgtttact
      421 a
//
LOCUS       DADTFG010000443          414 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.456, whole genome shotgun sequence.
ACCESSION   DADTFG010000443 DADTFG010000000
VERSION     DADTFG010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.456"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>414
                     /locus_tag="J1138_004895"
     CDS             <1..>414
                     /locus_tag="J1138_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456547.1"
                     /translation="YLSEDYTEEYKKKESWDDAHNDAYQNYQLKTPSIQMYAPVTSMI
                     LTRRRYFMECGGNNNEFHGHGYEDFEALNRLANRANKFSRSRDYYNHDFKYDSPHFCG
                     YRTFFSLFGRQLMNERVFFVHFWHPHNIAPSYAKRN"
BASE COUNT          139 a           58 c           77 g          140 t
ORIGIN      
        1 tatttaagtg aggattatac agaggaatac aaaaaaaaag aatcttggga tgatgcacat
       61 aatgatgctt atcaaaatta tcaattaaaa acaccaagta tccagatgta tgctcctgtt
      121 acgtcaatga tcttaactag gcggcgatac tttatggaat gtggaggtaa caataatgaa
      181 tttcatggac atggatatga agattttgag gcattgaaca gattagccaa tagagctaat
      241 aagttttctc gttctcgaga ttattataat catgatttta aatatgattc acctcatttc
      301 tgtgggtata gaacattttt tagtttattt ggtcgccaat taatgaatga gagagtgttt
      361 tttgtccatt tttggcatcc tcataatatt gcgccatctt atgctaaacg aaac
//
LOCUS       DADTFG010000444          399 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.458, whole genome shotgun sequence.
ACCESSION   DADTFG010000444 DADTFG010000000
VERSION     DADTFG010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.458"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          132 a           72 c           76 g          119 t
ORIGIN      
        1 tattaatatt tcccgatctg tattcaatga ttcgctcaaa gattgaaatt caagagaaat
       61 tgttatagca gtgatacgaa acaacaaaac ggaatgattg ccagccgtta gaaaacaccg
      121 gacactctcg tgtaaaaagc taccctgtct atggatatag cgatatcaac tcatcaagcg
      181 tggactgaat gatattcagc tccatatggg cggcaacctg gaggataaac atgatttcgc
      241 cgttggaatc aaggttgttc tgcaatatct accagactgc ttcaatttat ctgttttggc
      301 agtgccataa tcttaattta actatttgaa gatgatgata ttgtaagatg ccagaaattt
      361 agtaatctgg agaaccatgt ttacgatcac taaatatta
//
LOCUS       DADTFG010000445          397 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.459, whole genome shotgun sequence.
ACCESSION   DADTFG010000445 DADTFG010000000
VERSION     DADTFG010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.459"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            31..>397
                     /locus_tag="J1138_004896"
     CDS             31..>397
                     /locus_tag="J1138_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA8456548.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNTVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDD"
BASE COUNT          106 a           98 c           82 g          111 t
ORIGIN      
        1 gacataatct gacatctgaa ggtgcagttt atgaaacatt catttgaagt aaaacttgcc
       61 gctgtaaatc attacctggc tggtcatgca ggcatcattt ctacggcaaa actctttcaa
      121 ctttctcaca ccagcctctc acactggatt aatctttttc ttcttcacgg tcctcgggca
      181 ctggattgca gacacaagcg tagctattct cctgaagata aactttgcgt ggttctttat
      241 gctctcgggc attctgagtc tctaccccgg gtagctgccc ggtttaacat tcccagccac
      301 aacacggtta aaaactggat aaaaggctac cgcaaatctg gtaatgaggc ctttatcagg
      361 cgcaggaagg agaaaagcat gacccgttct gatgata
//
LOCUS       DADTFG010000446          389 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.460, whole genome shotgun sequence.
ACCESSION   DADTFG010000446 DADTFG010000000
VERSION     DADTFG010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.460"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>389
                     /locus_tag="J1138_004897"
     CDS             <1..>389
                     /locus_tag="J1138_004897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456549.1"
                     /translation="KKIKLFAIDSDGVILNDTYSPAIKIFVESFGVSYSPLIERSVWG
                     SPQVTAGHNLALACKLPYSGEKVIKDFFKTREEYLKKHPVEVLPDIESTLALLSSTGA
                     RITCYGGRNKEYSFDRFLKPFEKYFDK"
BASE COUNT          123 a           70 c           81 g          115 t
ORIGIN      
        1 tgaaaaaaat taaattattt gccattgaca gtgatggcgt tattctgaat gacacgtaca
       61 gcccggcgat caaaattttc gtggaatctt ttggcgttag ctattcacca ttaattgagc
      121 gcagtgtttg gggatcacca caagtgacag cagggcataa cctcgctctt gcctgtaaat
      181 taccttattc gggtgaaaaa gtcattaaag acttttttaa aaccagagag gaatacctga
      241 aaaaacatcc tgttgaagtt cttcctgaca ttgaatcaac gctggctttg ctaagtagta
      301 ctggagcacg tattacctgc tacggcggaa gaaataaaga gtacagcttc gataggtttt
      361 taaaaccttt tgaaaaatat tttgataag
//
LOCUS       DADTFG010000447          380 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.461, whole genome shotgun sequence.
ACCESSION   DADTFG010000447 DADTFG010000000
VERSION     DADTFG010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.461"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..308
                     /locus_tag="J1138_004898"
     CDS             <1..308
                     /locus_tag="J1138_004898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456550.1"
                     /translation="LTDENHQTVKVKIVKGKTTQQEILAAFGEPQTRATNDGQEMWNY
                     SSMTGESQLSNYIPGLALLTNSSTAHIKSLDIWFKGDVVERYDFRQTASKTSRGLMD"
BASE COUNT          124 a           75 c           81 g          100 t
ORIGIN      
        1 ccctaactga tgaaaatcat cagacggtta aagttaaaat cgtcaaggga aaaacaaccc
       61 aacaagaaat tcttgctgcc tttggagagc ctcagacacg cgctacgaat gatggacagg
      121 agatgtggaa ctactcaagt atgacgggag aaagccaact atctaactac ataccgggcc
      181 ttgctctatt gaccaatagc agcacagcac acattaaatc actggatatc tggtttaagg
      241 gtgatgttgt ggaacgttat gattttcgtc agactgcaag taagacctca cgtggtttga
      301 tggattgata atacaacata tcatcgtaaa atcgggagtt gctacgtatg aactcccgat
      361 tttatttcat gatattatat
//
LOCUS       DADTFG010000448          361 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.462, whole genome shotgun sequence.
ACCESSION   DADTFG010000448 DADTFG010000000
VERSION     DADTFG010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.462"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..232
                     /locus_tag="J1138_004899"
     CDS             <1..232
                     /locus_tag="J1138_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA8456551.1"
                     /translation="PVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGK
                     RIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT           96 a          100 c           83 g           82 t
ORIGIN      
        1 gcccgtcacc accgccgaca ttctgacgcc acgacgccgg gaggattacg gtaaggacct
       61 gtggagtgct tatcagacca ttcaggagaa tatgctgaaa ggcggaattt caggtcgcag
      121 cgctaaagga aaacgtatcc atacccgtgc cattcacagc attgacactg acattaagct
      181 caaccgcgca ttgtgggtga tggcggaaac cctgctggag agcctgcgct gattcagcca
      241 ttaccctgaa aaccacgcat ttatttgtcc tttaaggagt catttcatga aaaccatttc
      301 acataattcc accacatctt ctgtttccgt aaccgctgca tcagggaatg accagcctca
      361 g
//
LOCUS       DADTFG010000449          343 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.463, whole genome shotgun sequence.
ACCESSION   DADTFG010000449 DADTFG010000000
VERSION     DADTFG010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.463"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(50..>343)
                     /locus_tag="J1138_004900"
     rRNA            complement(50..>343)
                     /locus_tag="J1138_004900"
                     /product="23S ribosomal RNA"
BASE COUNT           85 a          100 c           69 g           89 t
ORIGIN      
        1 catgaagcca tcagaaccaa tcttggaacg tcgctgaaaa tctctctaaa aacaccttcg
       61 gtgttgtaag gttaagcctc acggatcatt agtactggtt agctcaatgc atcgctgcac
      121 ttacacaccc agcctatcaa cgtcttagtc ttaaacgttc cttcaggggc cttaaaggcc
      181 cagggaagac tcatcttgag gcaagtttcg cgcttagatg ctttcagcgc ttatcttttc
      241 cgcacttagc taccgggcaa tgccattggc atgacaaccc gaacaccagt ggtgcgttca
      301 ctccggtcct ctcgtactag gagcaacccc tctcaatctt cca
//
LOCUS       DADTFG010000450          340 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.465, whole genome shotgun sequence.
ACCESSION   DADTFG010000450 DADTFG010000000
VERSION     DADTFG010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.465"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..210
                     /locus_tag="J1138_004901"
     CDS             <1..210
                     /locus_tag="J1138_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBA8456552.1"
                     /translation="SRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFRWVTNIQEHQ
                     RYYYAFDNSLLFTESIQKTTQILPR"
BASE COUNT          129 a           74 c           50 g           87 t
ORIGIN      
        1 tccagaaaca aacatcttat agagacctcc tataataagt tgaaatcaaa agaaatcaca
       61 tttcaacaat acagggaaaa tcttgctaaa gcaggagttt tccgatgggt tacaaatatc
      121 caggaacacc aaagatatta ctatgccttt gataactcat tactatttac tgaaagcatc
      181 cagaaaacta cacagatctt accacgctaa accataacgt ccggcttctc tcactcctga
      241 gccggactgc attggtttaa taaaaaccat caacaattgt gatttagata ttcggaacca
      301 ttcaaatata acaaaacccc gtaaaaacgg ggtttatgga
//
LOCUS       DADTFG010000451          331 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.468, whole genome shotgun sequence.
ACCESSION   DADTFG010000451 DADTFG010000000
VERSION     DADTFG010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.468"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..227)
                     /locus_tag="J1138_004902"
     CDS             complement(<1..227)
                     /locus_tag="J1138_004902"
                     /inference="COORDINATES: protein motif:HMM:NF015437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456553.1"
                     /translation="MIKKIIEEYLKQIHADKYSVITINHLDEVSIISNLPYHWAQDFI
                     KRKLHLRSHIVLKAKNKITPFKWSSVNTANE"
BASE COUNT           96 a           62 c           51 g          122 t
ORIGIN      
        1 tcttcgttag ctgtatttac cgaggaccac ttgaaaggtg ttattttatt tttcgccttt
       61 aatacgatat ggcttcgtaa atgcaatttc ctttttataa aatcctgcgc ccagtgatat
      121 ggcagatttg agataatcga tacctcatca agatgattga tcgtaattac tgaatattta
      181 tctgcgtgta tttgcttaag atattcctct attatctttt taatcactac gacaccttgc
      241 atgttgaatg ataaaaaatt taatgttaag gaagctctgc ttcacgacta tatagatgca
      301 tcttatcaca cccagacatt gacatccatt a
//
LOCUS       DADTFG010000452          329 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.469, whole genome shotgun sequence.
ACCESSION   DADTFG010000452 DADTFG010000000
VERSION     DADTFG010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.469"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..>329)
                     /locus_tag="J1138_004903"
     CDS             complement(<1..>329)
                     /locus_tag="J1138_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA8456554.1"
                     /translation="SQTVPVSIIVIDDNSEKWCSAEKIVQEQNKFSDITIITHKNNQG
                     GGAARNSGIIKAKTQYIAFLDCDDYWDEDKIEKQEVLFENIKNEKAIIFSSIKVVNEN
                     LDVIKLY"
BASE COUNT           92 a           63 c           39 g          135 t
ORIGIN      
        1 atacaattta atcacatcta aattttcatt aactactttt attgatgaaa aaattattgc
       61 tttttcattt tttatgtttt cgaatagtac ttcttgcttt tcaattttat cttcatccca
      121 gtaatcatca caatccaaaa aagcgatata ttgtgttttt gctttgataa tgccactatt
      181 cctagcagcg cccccccctt gattgttttt atgcgtgatt atcgtaatat cggagaattt
      241 attttgttct tgaactattt tctccgccga acaccatttt tctgaattat catcgatgac
      301 aataatacta acaggtacag tttggctac
//
LOCUS       DADTFG010000453          317 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.471, whole genome shotgun sequence.
ACCESSION   DADTFG010000453 DADTFG010000000
VERSION     DADTFG010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.471"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>317
                     /locus_tag="J1138_004904"
     rRNA            <1..>317
                     /locus_tag="J1138_004904"
                     /product="23S ribosomal RNA"
BASE COUNT           90 a           67 c           99 g           61 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg tgaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgtttcg acacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg aggagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgc
//
LOCUS       DADTFG010000454          312 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.472, whole genome shotgun sequence.
ACCESSION   DADTFG010000454 DADTFG010000000
VERSION     DADTFG010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.472"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            65..140
                     /locus_tag="J1138_004905"
     tRNA            65..140
                     /locus_tag="J1138_004905"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Glu,seq:ttc)
BASE COUNT           87 a           72 c           74 g           79 t
ORIGIN      
        1 agtgctcaca cagattgtct gatagaaagt gaaaagcaag gcgtcttgcg aagcagactg
       61 acacgtcccc ttcgtctaga ggcccaggac accgcccttt cacggcggta acaggggttc
      121 gaatccccta ggggacgcca cttgctggtt tgtgagtgaa agtcacctgc cttaatatct
      181 caaaactcat cttcgggtga tgtttgagat atttgctctt taaaaatctg gatcaagctg
      241 aaaattgaaa cactgaacaa cgagagttgt tcgtgagtct ctcaaatttt cgcaacacga
      301 tgatgaatcg ca
//
LOCUS       DADTFG010000455          306 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.473, whole genome shotgun sequence.
ACCESSION   DADTFG010000455 DADTFG010000000
VERSION     DADTFG010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.473"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            1..306
                     /locus_tag="J1138_004906"
                     /pseudo
     CDS             1..306
                     /locus_tag="J1138_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           89 a           72 c           84 g           61 t
ORIGIN      
        1 tgagctgcgt tatcttcgcg cagaaaatgc atatctgaaa gccatgcagg agcatcttct
       61 ggaaaaaaag cgccaggagc tggagaaaaa acgaaagtca tccagagcct gaggtacgga
      121 cactgccagt ctgacctgtt aaaggccgca gggctggcca gaagcacgct gtactaccag
      181 ctgtcactgc agaaggctaa ggacaagtat gctgatgtga aacagctcat agcctccatc
      241 ttccatgaac accggggatg ttatgggtac cggcggatcc attgtgagct tcagaaaaga
      301 ggtctt
//
LOCUS       DADTFG010000456          304 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.474, whole genome shotgun sequence.
ACCESSION   DADTFG010000456 DADTFG010000000
VERSION     DADTFG010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.474"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            47..>304
                     /gene="hcp"
                     /locus_tag="J1138_004907"
     CDS             47..>304
                     /gene="hcp"
                     /locus_tag="J1138_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HBA8456555.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTTESIGNRWQSGHEDEIFTFSL
                     LNSITNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFME"
BASE COUNT          105 a           46 c           59 g           94 t
ORIGIN      
        1 atattgccat tgataaaata gcatgccata aaaggacttt tcagggatga gtaatattgt
       61 ttatctgaca gtaacgggag aacaacaagg aagcatctcc gcaggttgtg ggactactga
      121 atctataggt aatcgctggc agagcgggca tgaggatgaa atatttacat tctcactctt
      181 aaatagtatt actaatacgg ggcttggttc acagttccat ggtataacat tttgtaaatt
      241 aattgataaa agcactccat tatttattaa ttccattaac aataatgaac aattatttat
      301 ggaa
//
LOCUS       DADTFG010000457          302 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.475, whole genome shotgun sequence.
ACCESSION   DADTFG010000457 DADTFG010000000
VERSION     DADTFG010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.475"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            48..124
                     /locus_tag="J1138_004908"
     tRNA            48..124
                     /locus_tag="J1138_004908"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
     gene            167..242
                     /locus_tag="J1138_004909"
     tRNA            167..242
                     /locus_tag="J1138_004909"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Ala,seq:tgc)
BASE COUNT           81 a           68 c           78 g           75 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg caccgcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc atagctccac
      241 catctctgta gtgattaaat aaaaaatact tcagagtgta cctgcaaagg ttcactgcga
      301 ag
//
LOCUS       DADTFG010000458          293 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.478, whole genome shotgun sequence.
ACCESSION   DADTFG010000458 DADTFG010000000
VERSION     DADTFG010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.478"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          114 a           71 c           28 g           80 t
ORIGIN      
        1 tcctatatac aaggcacatc tccagaatat aaaagcacag acaaacaacc taaaaaaaca
       61 accccataca tataaattat tttataggta aaatagatta tatattctca tagctaccat
      121 aaactatact agcctgaact atatttattc tgctgcaatc aatgcataca taacacaaat
      181 atcactcagg tacactactc aaaccacgct gggatttttc ctcaagttat aatcatcacc
      241 ccgaaaatca ttcggcattt actcattaaa tagtcacccc ataggcctgt aca
//
LOCUS       DADTFG010000459          289 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.479, whole genome shotgun sequence.
ACCESSION   DADTFG010000459 DADTFG010000000
VERSION     DADTFG010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.479"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>289
                     /locus_tag="J1138_004910"
     CDS             <1..>289
                     /locus_tag="J1138_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456556.1"
                     /translation="ESLALAHPEIDIKGLWHCDSKYYVLIEKDGILTEDDLKEFEKIQ
                     EEHRIIGSPKLLLTQTLPNNAIKIAGRENYEVALSFGAPYTDSELENILYQ"
BASE COUNT          109 a           46 c           52 g           82 t
ORIGIN      
        1 ttgaatcgct tgcattagct catcccgaaa tagatataaa gggattatgg cattgtgatt
       61 caaaatatta tgtgttgatt gaaaaggatg gaatactcac tgaggatgac ttaaaggaat
      121 ttgaaaagat acaagaagag catcgtatta ttggtagccc taaattatta ctaacacaaa
      181 cactccctaa taatgcaata aaaatcgcag gaagagaaaa ctacgaagtt gctctttctt
      241 ttggcgcacc atacacagat tctgagttag aaaacatact ttatcaata
//
LOCUS       DADTFG010000460          287 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.480, whole genome shotgun sequence.
ACCESSION   DADTFG010000460 DADTFG010000000
VERSION     DADTFG010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.480"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..>287)
                     /locus_tag="J1138_004911"
     CDS             complement(<1..>287)
                     /locus_tag="J1138_004911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456557.1"
                     /translation="NFVNSTDNDAYTANVTPDHIIGVSPDKYVDVVTIRIGQRFVCKP
                     MTGKDSKVSSLCHELTHLVRYGPNGMYGGMQSEDMPVDKELQNAKEYDIFA"
BASE COUNT           83 a           61 c           51 g           92 t
ORIGIN      
        1 cagcaaagat gtcatactct ttcgcattct ggagctcttt atcaacaggc atatcctctg
       61 attgcatacc gccatacata ccgttggggc catatctgac taaatgtgtt aattcatggc
      121 aaagtgatga aactttagaa tcctttcctg tcattggttt acatacaaac cgctggccta
      181 ttcttattgt cactacatct acatatttgt ctggagaaac acctataata tgatcagggg
      241 tgacgttagc agtataagca tcattgtccg tagaatttac aaaattg
//
LOCUS       DADTFG010000461          287 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.481, whole genome shotgun sequence.
ACCESSION   DADTFG010000461 DADTFG010000000
VERSION     DADTFG010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.481"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..244
                     /locus_tag="J1138_004912"
     CDS             <1..244
                     /locus_tag="J1138_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HBA8456558.1"
                     /translation="SVSGFMGYLKGKSSLMPYEQFGDLKFKYRNREFWCRGYYVDTVG
                     KNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT           84 a           49 c           88 g           66 t
ORIGIN      
        1 gagcgtatca ggctttatgg gatatctgaa agggaaaagc agtctgatgc cttacgagca
       61 gtttggtgat ttgaaattca aatacaggaa cagggagttc tggtgcagag ggtattacgt
      121 cgatacggtg ggtaagaaca cggcgaagat acaggattac ataaagcacc agcttgaaga
      181 ggataaaatg ggagagcagt tatcgattcc ctatccgggc agcccgttta cgggccgtaa
      241 gtaacgaagt tggatgcaaa tgtcagatcg tgtgcgcctg ttagggc
//
LOCUS       DADTFG010000462          286 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.482, whole genome shotgun sequence.
ACCESSION   DADTFG010000462 DADTFG010000000
VERSION     DADTFG010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.482"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          104 a           45 c           62 g           75 t
ORIGIN      
        1 aaaataagat acaaactgaa gtaaagaaat caagaagagg tctcccacgt catactaaaa
       61 acccatttct taatgataca agtctacata caaaaactgg cgtcagacgt attactacag
      121 gaaaagatcg attagcatta gttaatgaga gtacgggaga acaagttggt aatggtggtt
      181 tctttcagtc tatggaagta gacaagacac agttcgtgaa gctttatgtg gatggcgtat
      241 cagctattga gggtttatcc tcatccggta agaaagtatt caagat
//
LOCUS       DADTFG010000463          286 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.483, whole genome shotgun sequence.
ACCESSION   DADTFG010000463 DADTFG010000000
VERSION     DADTFG010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.483"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          102 a           37 c           54 g           93 t
ORIGIN      
        1 tgattatgaa tcagtacgga ttattgagtt gtcgcaaata atcacggagc ttattgaagt
       61 tgtttataac agaaaggtgg acagaagttc atttaactat tccagattta tgatgcacct
      121 taaatatttt tcaagtcgcg tgttatgcaa tgaaaaaata aaacagaaag atattggtga
      181 tatctatgaa cagtttcttg aaaaggacat cctgttacag cgcgcaattc atgaaattga
      241 acggtatctg tatgccactt ttaaatatga attaatttta gaagaa
//
LOCUS       DADTFG010000464          281 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.484, whole genome shotgun sequence.
ACCESSION   DADTFG010000464 DADTFG010000000
VERSION     DADTFG010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.484"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>281
                     /locus_tag="J1138_004913"
     CDS             <1..>281
                     /locus_tag="J1138_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA8456559.1"
                     /translation="INKLIKDFGYDIELSIVGAGPQYDSLSILIRQYDLQDSIFLLGE
                     LDKKQISELMLSSDLFILLSRVETFGVVIIEGLATGLYCVATKCGGPEY"
BASE COUNT           86 a           36 c           63 g           96 t
ORIGIN      
        1 gataaataaa ttgattaaag attttggtta tgatatagag ttgagtattg ttggtgcggg
       61 tccacaatat gattcgctat caattctcat tcgacaatat gacttacaag attcaatatt
      121 tctactgggg gaactcgata aaaagcaaat atctgaattg atgctctcaa gcgatttgtt
      181 tattttgcta agtcgagtag aaacttttgg ggttgttatt attgaggggt tagcaaccgg
      241 attatattgc gttgcaacta aatgtggcgg gccagaatat a
//
LOCUS       DADTFG010000465          279 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.485, whole genome shotgun sequence.
ACCESSION   DADTFG010000465 DADTFG010000000
VERSION     DADTFG010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.485"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT          114 a           51 c           34 g           80 t
ORIGIN      
        1 tttcaatctg ttaaaaaatt tttcgctaca ctggcaagca aaaatgaaac aggaataatc
       61 gaaatgggat gttgcgcaca gttaaaataa ctcaccgtaa ataatcatct gctataaata
      121 atcgctttaa tgcaataccc gataagccat ttttaaacaa acacttaccg cacaacaaac
      181 taattaacta caaagaaagg caacaatcaa cctaatgtta ataaaatgtt ttgcatatat
      241 taaccacttg ttcaatatgg gaaatcagta cattatttt
//
LOCUS       DADTFG010000466          272 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.486, whole genome shotgun sequence.
ACCESSION   DADTFG010000466 DADTFG010000000
VERSION     DADTFG010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.486"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT           96 a           62 c           33 g           81 t
ORIGIN      
        1 aaaacatcac caagtgttaa aactaacaca acaattaacc aaaataatat ggctgtttat
       61 ttttcatccg taaacaaaaa ggtacaaata ttttcaccac aaaaaaacca ccagcatggc
      121 attgaattac cattcacaat acacacctcc tttcgcttat ttatgctcat tggttgacta
      181 ataatgatct ataccccctg ggcaactatc tctctggcgg ggtattgatg aatatccatt
      241 tagtggcata atttaaaccc cagctcatta tt
//
LOCUS       DADTFG010000467          248 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.491, whole genome shotgun sequence.
ACCESSION   DADTFG010000467 DADTFG010000000
VERSION     DADTFG010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.491"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..>248)
                     /locus_tag="J1138_004914"
     rRNA            complement(<1..>248)
                     /locus_tag="J1138_004914"
                     /product="23S ribosomal RNA"
BASE COUNT           56 a           80 c           54 g           58 t
ORIGIN      
        1 gacctacttt cgtacctgct cgagccgtca ctctcgcagt caagctagct tatgcctttg
       61 cactaacctc acgatgtccg accgtgatta gctaaccttc gtgctcctcc gttactcttt
      121 gggaggagac cgccccagtc aaactaccca ccagacactg tcctcacccc ggattacggg
      181 gccgagttag aacatcaaac attaaagggt ggtatttcaa ggatggctcc acgcagactg
      241 gcgtccac
//
LOCUS       DADTFG010000468          248 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.492, whole genome shotgun sequence.
ACCESSION   DADTFG010000468 DADTFG010000000
VERSION     DADTFG010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.492"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT           94 a           41 c           40 g           73 t
ORIGIN      
        1 ttattaaact ttgaactatc tgaaaaaagg cactctgatg cattagcgtt aatacctaat
       61 aaagcattac ctaaaagtgt cccaacaaga gtaagaaacc tgcatgaaga agatgaggat
      121 ttctggcttg ataacagaag tttaattctc acttcatatg acttcgaaaa aagtagtgcc
      181 cttccaacaa gttttaacaa aaaaaatagt tgttgtttcg ttgatgcgca aatttttaaa
      241 ccagataa
//
LOCUS       DADTFG010000469          243 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.493, whole genome shotgun sequence.
ACCESSION   DADTFG010000469 DADTFG010000000
VERSION     DADTFG010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.493"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..>243)
                     /locus_tag="J1138_004915"
     CDS             complement(<1..>243)
                     /locus_tag="J1138_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBA8456560.1"
                     /translation="IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPD
                     DDDDETWVLFNAMNGNRAEMSPEAAGIAACLMTYSH"
BASE COUNT           53 a           72 c           64 g           54 t
ORIGIN      
        1 tgatgactgt acgtcatcag acaggcagca atgccagcgg cttccgggct catttcagcg
       61 cggttgccgt tcatggcatt gaacagtacc catgtttcgt catcatcgtc atccggttcg
      121 ggggccataa atgccccgcc gttgttcaga gtgtacagat tccagatacc accgcagtag
      181 tcttcgcaca gacggtccat ccagccgaaa acacggggct ccagggtcac ccactgtgga
      241 atg
//
LOCUS       DADTFG010000470          239 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.494, whole genome shotgun sequence.
ACCESSION   DADTFG010000470 DADTFG010000000
VERSION     DADTFG010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.494"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
BASE COUNT           88 a           43 c           33 g           75 t
ORIGIN      
        1 accaaaaaaa caaaaatcat aaaattgaaa gcaatcaatt ttaatctacc tgcagtatct
       61 ataatgttgt tattcattta gacccttttg aaagccagct caaatgcagt cattaaaact
      121 cggagagttg tttttactcc gcactttcta gacaagttta gtaagaaaga aaacagctta
      181 cgttgtttca aactgaacca cataggcgtt tttactagaa aatagcaact agttttttc
//
LOCUS       DADTFG010000471          233 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.496, whole genome shotgun sequence.
ACCESSION   DADTFG010000471 DADTFG010000000
VERSION     DADTFG010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.496"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>233
                     /locus_tag="J1138_004916"
     CDS             <1..>233
                     /locus_tag="J1138_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA8456561.1"
                     /translation="NGLILMKLPETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKN
                     ARILGDNNARNGADRLVSGTEIQTKYCSTAARSV"
BASE COUNT           72 a           49 c           65 g           47 t
ORIGIN      
        1 aatggtctga ttctcatgaa actgccggaa acctggagta caaatgagaa aatgtttgct
       61 tccggagggc agggacatgg gtttgccgct gaacggggaa accatattgt cgacagagtc
      121 cgtctgaaaa acgcacggat cctcggtgat aataatgcca gaaatggagc agacagactg
      181 gtcagcggaa cagaaatcca gacgaaatat tgttcaactg cagcccgtag cgt
//
LOCUS       DADTFG010000472          229 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.denovo.497, whole genome shotgun sequence.
ACCESSION   DADTFG010000472 DADTFG010000000
VERSION     DADTFG010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.denovo.497"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            <1..>229
                     /locus_tag="J1138_004917"
     CDS             <1..>229
                     /locus_tag="J1138_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA8456562.1"
                     /translation="SHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQN
                     NADRLVIDGGRATGKTILNLVNAGNSASGLAT"
BASE COUNT           65 a           68 c           63 g           33 t
ORIGIN      
        1 agccatgccg gacagattca tttcacctcc acccgcacag ggaagttcgt accggcaacc
       61 ctgaaagtga aaaacctgaa cggacagaat ggcaccatca gcctgcgtgt acgcccagat
      121 atggcacaga acaatgctga cagactggtc attgacggcg gcagggcaac cggaaaaacc
      181 atcctgaacc tggtgaacgc cggcaacagt gcgtcggggc tggcgacca
//
LOCUS       DADTFG010000473        17389 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.guided.105, whole genome shotgun sequence.
ACCESSION   DADTFG010000473 DADTFG010000000
VERSION     DADTFG010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.guided.105"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="J1138_004918"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="J1138_004918"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBA8456563.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="J1138_004919"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="J1138_004919"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBA8456564.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="J1138_004920"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="J1138_004920"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBA8456565.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="J1138_004921"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="J1138_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBA8456566.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="J1138_004922"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="J1138_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBA8456567.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNTMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="J1138_004923"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="J1138_004923"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBA8456568.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="J1138_004924"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="J1138_004924"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBA8456569.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /locus_tag="J1138_004925"
     CDS             11328..12062
                     /locus_tag="J1138_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HBA8456570.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNARTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="J1138_004926"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="J1138_004926"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBA8456571.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="J1138_004927"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="J1138_004927"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBA8456572.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12901..14034
                     /gene="blaEC"
                     /locus_tag="J1138_004928"
     CDS             12901..14034
                     /gene="blaEC"
                     /locus_tag="J1138_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBA8456573.1"
                     /translation="MLKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPFDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            14123..14656
                     /locus_tag="J1138_004929"
     CDS             14123..14656
                     /locus_tag="J1138_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HBA8456574.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /locus_tag="J1138_004930"
     CDS             complement(14653..14970)
                     /locus_tag="J1138_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HBA8456575.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15146..15292)
                     /gene="ecnB"
                     /locus_tag="J1138_004931"
     CDS             complement(15146..15292)
                     /gene="ecnB"
                     /locus_tag="J1138_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBA8456576.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15403..15528)
                     /gene="ecnA"
                     /locus_tag="J1138_004932"
     CDS             complement(15403..15528)
                     /gene="ecnA"
                     /locus_tag="J1138_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBA8456577.1"
                     /translation="MMKRLIVLVLLASTLFTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15580..16146)
                     /gene="efp"
                     /locus_tag="J1138_004933"
     CDS             complement(15580..16146)
                     /gene="efp"
                     /locus_tag="J1138_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBA8456578.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16188..17216
                     /gene="epmB"
                     /locus_tag="J1138_004934"
     CDS             16188..17216
                     /gene="epmB"
                     /locus_tag="J1138_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBA8456579.1"
                     /translation="MAHIVTLNPPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4327 a         4556 c         4479 g         4027 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccaactcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaagccgtca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctaacatc gagatagcgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg gtcctgaccg atgctgttac
      481 cacaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacacgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccagc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttttacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggatga cgaaggcatg gagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgcac gactgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc cccaggtgtg cgtgagtttg gtctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaaggaaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggatctc ttccgtaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgta acggtattct gcgtgaaatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcat
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aacttggctc taccgttatc aacctgtttg
     2821 caccaggtaa agtgaacctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacca gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgac aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattatccg aaactttccg ccactctgcg cgcgcagtta aacaacatgc
     3301 gtgacgagcc acgcagcgtg tcgccgggaa tgtctaacga tgcgctgaat caggaaattc
     3361 tccagatcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttgaatga aatcgagcgc cgcctgggga cgcttaccgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgtctgaa agcgctcgtt gatgaactgg
     3601 agctggcgca gctatctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 cggaaaaaga gagccagcag ctggatgcat atttgcaagc actacgcaac cagttgaata
     3721 gtcaacgcca actggaagcg gaacgggcgc tggaaagtac cgagcaactg gcggaaagta
     3781 gcgccgattt gccaaaagat atcgtcgccc agtttaaaat taaccgcgaa ctatcggcgg
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcatctaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttacg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccac
     4141 tgaccgctga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggagct gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagtaagga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acactgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgctcgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgagtttc ccgtgggatg cgttactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccgctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggtttttgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca gcgcgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcggt actgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattctgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgtg
     6001 tggtgttgac aattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcgccggaa gtcttcctgg
     6121 tggatctgca acaggggatt caaattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc aactaattct ggcgggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctgaat ggtaaacaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 tggccccgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttgtt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccagct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc actgttgcaa tcattgccga catatgcgtt
     6721 ttgaaaatca caaacggtct gtcgagatcc tgacgtgctt taaaaaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagaa tgaagatagt aaccaacccg
     6901 cactgcatcc acatggcaat agacggcatc cccatcgtat tcaggcgcgt catcggttcc
     6961 ggccacaatg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagtgta
     7021 aagaacgcac cggtataagc gaggaacatc gacagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt cacccctagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatgg caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat cccttttgaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatatgcca gtgctgggtc atgtcgctac cgtagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtactcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagataccg
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacagaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgagtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac
     8041 gcgcccactg aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtgatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttgcc aatatttggc tctacgccaa aggtaaacag caattgtaga agcgtgtcgc
     8641 ggtcttcttc agtatcggca acgttgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagt gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccgga cagtccagca cctgttgcaa gagatcgtcc acctcgttca
     8821 tcaatcggta catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaagc tgcggcacag ctggaatacc ggcccacaac
     8941 cggcaaccag caggcgcttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaataa aacaacggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctaccggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctacca ggttccggta tcctgatgac
    10261 cgaaggctgc cgcggtgaag gtggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgcca cgtggcgatg tggtttacct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcgaaccgtc tgggctccaa ctcgctggcg gaactggtgg tcttcggtcg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggctaaga tccgcgacga aatgggcatg gccatggaag aaggctgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ttccagcgtg ttcaacaccg acctgctcta
    11041 caccattgag ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cgcaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgtgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggct
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaacg
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttatcg tgatcattaa cctgatcact
    12301 ctggcggcag ccctgctgca caccaaaacc tggtttgagc tggcaccaaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgccatca ttgcgccggt gatgatcctg ctggttggta ttctgctgcc
    12601 gctagggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aattcacgta cctgcgggta aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg
    12841 catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagacctt
    12901 atgctcaaaa cgacgctctg caccttatta attaccgcct cttgctccac atttgccgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccacaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc
    13261 acactattac atctcgcaac ctacaccgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg aaaactcgtg tcttccagcc actcaaactc
    13501 aatcatacgt ggattaatgt accgtccgca gaagaaaaga actacgcctg gggatatcgc
    13561 gaaggtaagg cagtacatgt ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag
    13621 tcgaccattg aagatatggc ccgctgggtg caaagcaatt taaaaccctt tgatatcaat
    13681 gagaaaatac ttcaacaagg gatacagctg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gtctgggctg ggaaatgctg gactggccgg taaatcctga catcatcatt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaaccgat tacgccccca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagttac cgccgcctgg cagattctca acactctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctcc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttcccctac gccgccgcgt ggcgtgaccg tagtgaataa
    14221 tttcgacgct aaacgttatc tcggtacctg gtatgagatt gcccgttttg atcaccgctt
    14281 tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag agaaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggccc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca
    14641 gcagcccggt agttaatgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt
    14821 catcgcccag gcaagtagcg ccagactgac aatcatcgcc gtcacggtaa taacacttgg
    14881 cgtcaaacga ctaaagccgt gagtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggaaaattgg ggccgtcccc
    15001 ggtgaaaaga cgcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattaccta tgtaactatc gcagaaaaaa caaacggcac
    15121 gacacagctg tcgtaccgtt attgcttatt gctgcgcttt cgttgctgcg ccagaaatcg
    15181 cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata
    15241 ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt
    15301 ccttttaggt aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca
    15361 aaaatgatgg aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcggga
    15421 gatggagttg ccgaggtgtt tgatatcttc gccgaaaccg cgagcggtgt tacagcccgt
    15481 aaacagcgtg ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa
    15541 taagtgatgg tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta
    15601 ttcaccagag cgggtatcca ctttgatgac ttcgccgatt tgtacaaaca gcggaacttt
    15661 aaccacagcg ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt
    15721 caggcccgga tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat
    15781 cggctgacca ttccacagag ttacgataca ctctgcctga tccagcaacc atttagcgtt
    15841 gtcgccaatt gcttttgcgt cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg
    15901 ccagaactca ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc
    15961 agcggaatca gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag
    16021 tttaacgcga gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc
    16081 gtaaggttcg ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt
    16141 tgccataagg ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg
    16201 taaccctaaa tcccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg
    16261 atcctgatga acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac
    16321 gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga
    16381 aaggcaatcc ggacgatcct cttttgcgtc aggtacttac ctcgcaagat gagtttgtcg
    16441 tcgcgcccgg attctccacc gacccgctgg aagaacagca tagcgtagtg cctggtttgt
    16501 tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc
    16561 gctattgctt ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag cgtaactggc
    16621 agactgcact tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg
    16681 gcgatccgct gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca
    16741 tcccgcatat caaacgtctg cggattcaca gccgtctgcc gattgtgatc ccggcacgca
    16801 tcacagatgc gctggttgaa cgcttttctc attctacgct gcaaatcttg ctggtgaatc
    16861 acatcaacca tgccaatgag gtagatgaaa cattccgtca ggcgatggct aagttgcgcc
    16921 gggtgggcgt tactttgctg aaccagagcg ttctgttacg tggtgtgaac gataacgcgc
    16981 aaacgctggc aaacctgagt aatgcgttgt tcgatgcggg cgtgatgccg tattacctgc
    17041 atgtgctcga taaagtacag ggcgcggcgc attttatggt gagtgatgac gaagcacggc
    17101 agattatgcg tgagttgctg acattggtgt cgggatatct ggtgcctaaa ctggcgcgag
    17161 aaattggcgg cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga
    17221 ttaaacaggg aagtttaatc tttgcccgtt gcataacctt taattattca tatacattgc
    17281 tgttgttact ctttatataa ccaacaccaa taattaacct ttcttttctg tttatataga
    17341 gtgagcacgg ctatttatgt tattttaaca actattacta ccaagccca
//
LOCUS       DADTFG010000474         4142 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2187
            SAMN08039556-rid11721263.guided.238, whole genome shotgun sequence.
ACCESSION   DADTFG010000474 DADTFG010000000
VERSION     DADTFG010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039556
            Sequence Read Archive: SRR6362291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000979920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000979920.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 00:40:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 69 of 144
            Pseudo Genes (incomplete)         :: 65 of 144
            Pseudo Genes (internal stop)      :: 41 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039556-rid11721263.guided.238"
                     /strain="LGE2187"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.9028 N 95.9074 W"
                     /collection_date="2015-08-17"
     gene            complement(<1..440)
                     /locus_tag="J1138_004935"
     CDS             complement(<1..440)
                     /locus_tag="J1138_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBA8456580.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEA"
     gene            701..1159
                     /gene="gpt"
                     /locus_tag="J1138_004936"
     CDS             701..1159
                     /gene="gpt"
                     /locus_tag="J1138_004936"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBA8456581.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            1251..2501
                     /gene="frsA"
                     /locus_tag="J1138_004937"
     CDS             1251..2501
                     /gene="frsA"
                     /locus_tag="J1138_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBA8456582.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPGPFPTVLMCGGLDAMQTDYYSLYER
                     YFAPRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRF
                     GANVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASD
                     EALRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIP
                     FNPVYRNFDKGLQEITDWIEKRLC"
     gene            2559..2960
                     /locus_tag="J1138_004938"
     CDS             2559..2960
                     /locus_tag="J1138_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBA8456583.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAKVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(2999..4054)
                     /gene="phoE"
                     /locus_tag="J1138_004939"
     CDS             complement(2999..4054)
                     /gene="phoE"
                     /locus_tag="J1138_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBA8456584.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMMPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1023 a         1010 c         1025 g         1084 t
ORIGIN      
        1 gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt ttcgtcagcc
       61 agaaccgcca gtgcagaggc cataccaatg ccgttatccg cacccagcgt ggtgccgcgt
      121 gctttaaccc attcgccatc aatataaggc tggataggat ctttagtgaa gtcatgcacg
      181 gtgtcgttgt ttttttgcgg caccatatcg aggtgggcct gcaagacgac cggtttacga
      241 ttttccatac ctgcggtagc aggtttacga atcaggatat tacctacctg atcgcgttcg
      301 acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg ctcttcatga
      361 taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg ctgtggagat
      421 aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa cagtattgca
      481 ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga tatgtttgag
      541 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
      601 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
      661 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
      721 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
      781 acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccgggtg cgttactggc
      841 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
      901 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
      961 tgatgacctg gtagataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     1021 gcactttgtc accatcttcg caaaaccggc cggtcgtccg ctggttgatg attatgttgt
     1081 tgatattccg caaaatacct ggatcgaaca gccgtgggat atgggcgtcg tattcgtccc
     1141 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     1201 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     1261 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     1321 tccgccgctt tagtcacggc gcacaactgc ctgtgcagtc ggcccttgat ggtaaaacca
     1381 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     1441 gtgaaatcct cgatgtccag gcgcgtattg tgatgagcga tgccgaacgt accgacgatg
     1501 atttatacga tacggtgatt ggctaccgcg gcggcaactg gatttatgag tgggctaccc
     1561 aggcgatggt gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc agcggtcgac
     1621 actggctgca tgcggctacg ttgtacaaca ttgctgccta tcctcatctg aaaggagatg
     1681 acctggccga gcaagcgcag gctttgtcaa accgcgccta tgaagaggcc gctcagcgtc
     1741 tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc atcaccggct
     1801 ttttgcatat gccgaaaggc gatggccctg gcccgttccc gacagtatta atgtgtggtg
     1861 gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg
     1921 gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca
     1981 cccaggactc cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg
     2041 tagatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc
     2101 tggcatacct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata
     2161 ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg
     2221 cgagccgttt ggggatgcat gatgcttccg acgaagcgtt gcgcgtggag ctgaatcgct
     2281 attcattaaa agtgcaagga ttgctgggac gtcgctgccc aacgccaatg ttatcaggct
     2341 actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg
     2401 ctgacggtaa attgttagag atcccattta acccggtgta tcggaatttt gacaaaggcc
     2461 ttcaggaaat cacagactgg atcgaaaaac gtttgtgtta aaaatttgct aaattttacc
     2521 aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg agtggacacc
     2581 cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt
     2641 gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac
     2701 cggaagtgcg tgagttctgg ggctggtgga tggaactgga agcgcaggaa tcccgtttta
     2761 cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggacgagt gttcagataa
     2821 aagatgccaa agtggttgaa cgactggagc acaccctgcg tgaatttcac gagaagctgc
     2881 gtgagctgct ggcgacgctg aatctgaagc tggaaccggc agatgatttt cgtgatgaac
     2941 cggtgaagtt aacggcgtga atgaaatgtg ccggatgcat cacatccggc aatatccatt
     3001 aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc aatttgttat
     3061 cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg ttgaaataat
     3121 acgtagcacc aacatcgaca taattgacca gatcttcatc accgatgccc tcaatatctt
     3181 tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag
     3241 cgaccgcttc aaagttctgc gtcttattgg caaagccgcc agagattggc atcattttac
     3301 gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc agacctgttg
     3361 cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg ttggtacgat
     3421 cagagttggt ataggctcca ctgatggcga aatcgctgcc accaaagtca tatgtcaatg
     3481 acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat
     3541 attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg
     3601 ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggag tcgccaccaa
     3661 attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacgaccat
     3721 aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc gttttttgct
     3781 gtgcagtatc actctccgct ttattaccgg caaactcagc ttcccaacga ccataaccag
     3841 tcagttgatc gttaatttgt gtttcgcctt taaaaccaaa acggatataa ctctggtcgc
     3901 catctttact gtcattatca ctcatataat gcatggcttt aactttgcca tagacatcca
     3961 gtttattacc gtctttgtta tatatttctg cggcctgtac ggatacagat gccacaatgc
     4021 ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt aattaacgcg
     4081 cgaatattca gcgggagagt cccgttgaaa acaggaaagt tttttacctg agattgttaa
     4141 ag
//
